WorldWideScience

Sample records for papaya chilena vasconcellea

  1. Deshidratación osmótica de la papaya chilena (Vasconcellea pubescens) e influencia de la temperatura y concentración de la solución sobre la cinética de transferencia de materia Desidratação osmótica do mamão chileno (Vasconcellea pubescens) e influência da temperatura e concentração da solução sobre a cinética de transferência de matéria

    OpenAIRE

    Antonio Vega-Gálvez; Marlene Palacios; Francisca Boglio; Catarina Pássaro; Catalina Jeréz; Roberto Lemus-Mondaca

    2007-01-01

    El objetivo de este trabajo fue estudiar la cinética de deshidratación osmótica de la papaya chilena (Vasconcellea pubescens), utilizando dos variables experimentales: la temperatura (30, 40, 50 °C) y concentración (40, 50 y 60%) de la solución osmótica. Se determinó la variación de masa total, masa de agua y masa de sólidos solubles y se modeló con las ecuaciones difusionales, además en esta investigación se consideró y utilizó una modificación de la ecuación propuesta por Biswal-Bozorgmehr....

  2. Deshidratación osmótica de la papaya chilena (Vasconcellea pubescens e influencia de la temperatura y concentración de la solución sobre la cinética de transferencia de materia Desidratação osmótica do mamão chileno (Vasconcellea pubescens e influência da temperatura e concentração da solução sobre a cinética de transferência de matéria

    Directory of Open Access Journals (Sweden)

    Antonio Vega-Gálvez

    2007-09-01

    Full Text Available El objetivo de este trabajo fue estudiar la cinética de deshidratación osmótica de la papaya chilena (Vasconcellea pubescens, utilizando dos variables experimentales: la temperatura (30, 40, 50 °C y concentración (40, 50 y 60% de la solución osmótica. Se determinó la variación de masa total, masa de agua y masa de sólidos solubles y se modeló con las ecuaciones difusionales, además en esta investigación se consideró y utilizó una modificación de la ecuación propuesta por Biswal-Bozorgmehr. Estos modelos fueron evaluados por medio del coeficiente de regresión lineal, suma de errores cuadrados, raíz media de los errores cuadrados y Chi-cuadrado. Al comparar los valores experimentales con los calculados, se demostró que el modelo Biswal-Bozorgmehr modificado obtuvo mejor ajuste sobre la variación de masa de agua y sólidos solubles. Mediante la ecuación de Arrhenius se analizó el efecto de la temperatura sobre los parámetros cinéticos (Kw y Kss y difusividad efectiva de agua (Dwe y sólidos solubles (Dsse. De acuerdo al diseño factorial, se observó que la temperatura no influyó sobre la difusividad efectiva de agua y de sólidos, pero sí la concentración de la solución osmótica. La mejor condición de salida de agua y ganancia de sólidos solubles durante la deshidratación osmótica de la papaya chilena fue a 30 °C con una concentración del 60%.O objetivo deste trabalho foi estudar a cinética de desidratação osmótica do mamão chileno (Vasconcellea pubescens utilizando duas variáveis experimentais: temperatura (30, 40 e 50 °C e concentração (40, 50 e 60% da solução osmótica. Para este estudo, determinou-se a variação de massa total, de massa de água, de massa de sólidos solúveis, modeladas com as equações difusionais. Nesta investigação, também se considera e se usa uma modificação da equação proposta por Biswal-Bozorgmehr. Estes modelos foram avaliados através do coeficiente de regressão linear, da somatória de erros quadrados, da raiz média de erros quadrados e do Qui-quadrado. Comparando os valores experimentais com os valores calculados, demonstrou-se que o modelo Biswal-Bozorgmehr modificado apresenta um melhor ajuste sobre a variação da massa de água e de sólidos solúveis. Com a equação de Arrhenius, analisou-se o efeito da temperatura sobre os parâmetros cinéticos (Kw e Kss e a difusividade efetiva de água (Dwe e de sólidos solúveis (Dsse. De acordo com o desenho fatorial, observou-se que a temperatura não teve influência sobre a difusividade efetiva da água e dos sólidos solúveis, mas sim sobre a concentração da solução osmótica. A melhor condição de saída de água e ganho de sólidos durante a desidratação osmótica do mamão chileno foi a 30 °C com uma concentração de 60%.

  3. Papaya

    Science.gov (United States)

    Papaya is a plant. The leaves are used to make medicine. Papaya is used for preventing and treating gastrointestinal tract ... of the lymphatic system caused by parasitic worms. Papaya contains a chemical called papain, which is commonly ...

  4. Palynology of Carica and Vasconcellea (Caricaceae Palinología de Carica y Vasconcellea (Caricaceae

    Directory of Open Access Journals (Sweden)

    Lagos Túlio César

    2006-09-01

    Full Text Available

    Palynology of Carica and Vasconcellea (Caricaceae. The pollen of C. papaya and agreements of Vasconcellea cauliflora, V. cundinamarcensis, V. crassipetala, V. goudotiana, V. x heilbornii var. chrysopetala, V. longiflora and V. sphaerocarpa collected in the Colombian Coffee Growing Zone, using the technique of acetolisis for optic microscopy and the fixation procedure with glutaraldehide, dehydration and ionization with gold-palade, for scanning electronic microscopy was described. The pollen grains were characterized using descriptors, which include the characters of taxonomic value for pollen identification, genetically determined. The most important are the number, position and character of the aperture (NPC and exine ornamentation and stratification. The pollen is of medium size for both genera, tricolporate, zonoaperturate, prolate-spheroid to subprolate, isopolar radial symmetry, tectate, dug, foveolate, with columelas. These characters have demonstrated a great contribution to the taxonomy of Caricaceae because the cluster analysis allowed distinguish very well the two genera.

    Se describe el polen de C. papaya y accesiones de Vasconcellea cauliflora, V. cundinamarcensis, V. crassipetala, V. goudotiana, V. x heilbornii var. chrysopetala, V. longiflora y V. sphaerocarpa recolectadas en la Zona Cafetera de Colombia, empleando la técnica de acetólisis para microscopía óptica y el procedimiento de fijación con glutaraldehído, deshidratación e ionización con oro paladio, para microscopía electrónica de barrido (MEB. Se caracterizaron los granos de polen por medio de una lista de descriptores que incluyeron los caracteres de valor taxonómico para identificación del polen, genéticamente determinados, siendo los más importantes el número, posición y carácter de la abertura (NPC y la ornamentación y estratificación de la exina. Para los dos géneros el polen es mediano, tricolporado, zonoaperturado, prolato–esferoidal a subprolato, de simetría radial isopolar, tectado, cavado, foveolado, con columelas. Estos caracteres han demostrado gran aporte a la taxonomía de Caricaceae, ya que el análisis de agrupamiento permitió diferenciar bien a los dos géneros.

  5. Intestinal obstruction due to Vasconcellea seeds: Report of three cases and literature review

    Directory of Open Access Journals (Sweden)

    Montoya-González, Juliana

    2015-07-01

    Full Text Available Vasconcellea spp., is a species of Andean papaya commonly cultivated in rural communities close to Medellín, Colombia. Due to the pleasant and sweet flavor of its fruits, children frequently ingest its seeds accidentally. After ingestion, the seeds are engaged in the colonic lumen and block the exit of stools, causing pain and bloating, and promoting bacterial translocation. Diagnosis is based on clinical history and rectal examination. Treatment depends on the degree of local and systemic involvement and includes disimpaction of rectal contents under general anesthesia and colonic washes with 0.9% saline solution. In severe cases derivative colostomy has been required. In the literature there are no reports of intestinal obstruction due to Vasconcellea seeds, possibly because it has been mistaken for seeds of the genus Carica. In this article, three cases treated at pediatric services in Medellín, Colombia, in 2012 and 2013 are described. We note that this is a rarely suspected disease, leading to late diagnosis and potential catastrophic consequences. It is important to educate people to prevent the ingestion of the seeds.

  6. Citogenética de especies de Vasconcellea (Caricaceae

    Directory of Open Access Journals (Sweden)

    Caetano Creucí María

    2008-12-01

    Full Text Available Conocer aspectos básicos de la citogenética de las papayas de montaña o papayuelas, especies del género Vasconcellea (Caricaceae originarias de Ecuador y Colombia, fue el objetivo del trabajo. Botones florales se fijaron en etanol-acético 3:1 por 24h, luego se transfirieron a nueva solución y se almacenaron en frío. Los microsporocitos y los granos de polen se tiñieron con acetocarmín (1%. Se evaluaron las fases de meiosis y la viabilidad de polen para describir el comportamiento cromosómico. Se encontró que: 1 todas las seis especies estudiadas fueron diploides (2n=2x=18; 2 se describió el número cromosómico para V. sphaerocarpa, V. longiflora y V. palandensis (2n=18; 3 el grado de asimetría de los complementos cromosómicos indicó un proceso de evolución; 4 la presencia de numerosos NOR en V. sphaerocarpa está relacionada con los micronucleolos y corroboró el posible origen híbrido; y 5 V. cauliflora (42.98% y V. cundinamarcensis (47.93% presentaron la más baja viabilidad polínica.

  7. BASKETFUL BENEFITS OF PAPAYA

    OpenAIRE

    Parle Milind; Gurditta

    2011-01-01

    Papaya (Carica papaya linn) is well known for its exceptional nutritional and medicinal properties throughout the world. From the times immemorial, the whole Papaya plant including its leaves, seeds, ripe and unripe fruits and their juice is used as a traditional medicine. The fruit has a large oval shape, yellowish-green skin and yellow flesh. Nowadays, Papaya is considered as a Nutraceutical fruit due to its multi-faceted medicinal properties. The prominent medicinal properties of Papaya in...

  8. BASKETFUL BENEFITS OF PAPAYA

    Directory of Open Access Journals (Sweden)

    Parle Milind

    2011-07-01

    Full Text Available Papaya (Carica papaya linn is well known for its exceptional nutritional and medicinal properties throughout the world. From the times immemorial, the whole Papaya plant including its leaves, seeds, ripe and unripe fruits and their juice is used as a traditional medicine. The fruit has a large oval shape, yellowish-green skin and yellow flesh. Nowadays, Papaya is considered as a Nutraceutical fruit due to its multi-faceted medicinal properties. The prominent medicinal properties of Papaya include Anti-fertility, Uterotonic, Diuretic, Anti-hypertensive, Hypolipidemic, Anti-helmintic, Wound-healing, Anti-fungal, Anti-bacterial, Anti-tumor and Free radical scavenging activities. Phytochemically, the whole plant contains enzymes (Papain, carotenoids, alkaloids, monoterpenoids, flavonoids, minerals and vitamins. In the present review article, a humble attempt is made to compile all the strange facts available about this tasty fruit. This tasty fruit of Papaya is popular among family members of all ages for the delicious dishes derived from it.

  9. Post-irradiation identification of papaya ( Carica papaya L.) fruit

    Science.gov (United States)

    Chatterjee, Suchandra; Variyar, Prasad S.; Sharma, Arun

    2012-03-01

    Impact of radiation processing on the volatile essential oil profile of papaya ( Carica papaya) was investigated. Gamma-radiation processing resulted in the appearance of a new peak in the GLC profile that was identified as phenol. The observed dose dependent increase in phenol content suggested possible use of this compound as a marker for radiation processed papaya.

  10. Papaya: environment and crop physiology

    Science.gov (United States)

    Papaya (Carica papaya L.) is a principal horticultural crop of tropical and subtropical regions. Knowledge of how papaya responds to environmental factors provides a scientific basis for the development of management strategies to optimize fruit yield and quality. A better understanding of genotyp...

  11. en la adolescencia chilena

    Directory of Open Access Journals (Sweden)

    Luis Cárcamo Ulloa

    2008-01-01

    Full Text Available En medio de los cambios que generado la llamada Era de la Información, adquiere importancia el fenómeno de la Comunicación Mediada por Computador (CMC. Esto se relaciona con el creciente acceso de la sociedad chilena hacia la internet, que lo posiciona como líder en Latinoamérica en el tema. Dada la cercanía que los jóvenes tienen con las nuevas tecnologías, este grupo etáreo es el que se apropia con mayor intensidad de esta nueva herramienta comunicativa. La mensajería instantánea pasa a ser una importante herramienta para los procesos de socialización de los jóvenes, ocupando el lugar que en décadas pasadas tuvo el teléfono. A diferencia de otras tecnologías, la mensajería se convierte en uno de los pocos elementos comunes que cruza los segmentos sociales y las variadas tribus urbanas en las cuales los jóvenes chilenos viven sus procesos de construcción de la identidad.

  12. In vitro morphogenic responses of Vasconcellea chilensis Planch. ex A. DC (Caricaceae

    Directory of Open Access Journals (Sweden)

    Jordan Z. Miguel

    2011-12-01

    Full Text Available

    Multiple shoot formation was induced in vitro on nodal sections excised from adult “palo gordo” plants of Vasconcellea chilensis ex Carica chilensis, in the presence of relatively high levels of thidiazuron (TDZ, indole-3- acetic acid (IAA and organic addenda included in the WPM formulation including casein hydrolysate, adenine sulfate and cystein. Multiple shoots appeared simultaneously in strands or clusters on the surface of the explants; usually two or more clusters with new shoots were initiated, all showing synchronous growth. In subculture, in the presence of indole-3-butyric acid (IBA as single hormone, root formation did not take place; the shoots turned green instead, evidenced elongation and development of new shoots at the base of the explants. Other explants, such as petioles and leaf sections exhibited callus initiation upon the vascular system after 4-5 weeks; however, this tissue turned brown later on and died.

  13. Papaya ringspot virus (Potyviridae)

    Science.gov (United States)

    Papaya ringspot virus, a member of the family Potyviridae, is single stranded RNA plant virus with a monocistronic genome of about 10,326 nucleotides that is expressed via a large polyprotein subsequently cleaved into functional proteins. It causes severe damage on cucurbit crops such as squash and...

  14. Biodistribution, pharmacokinetics and toxicity of a Vasconcellea cundinamarcensis proteinase fraction with pharmacological activity

    Directory of Open Access Journals (Sweden)

    Fernanda O. Lemos

    2016-02-01

    Full Text Available Abstract Prior studies demonstrate that a proteinase fraction from Vasconcellea cundinamarcensis V.M. Badillo, Caricaceae, exhibits wound healing activity in gastric and cutaneous models and antitumoral/antimetastatic effects. Here, we present the toxicity, pharmacokinetics and biodistribution data for this proteinase fraction following a single dose into Swiss mice by i.v., s.c. or p.o. routes. The i.v. and s.c. toxicity assays demonstrate that proteinase fraction at ≤20 mg/kg is non-lethal after single injection, while parental administration (p.o. of ≤300 mg/kg does not cause death. Based on p.o. acute toxicity dose using Organisation for Economic Cooperation and Development protocols, proteinase fraction ranks as Class IV “harmful” substance. Proteinase fraction shows high uptake determined as Kp (distribution tissue/blood in organs linked to metabolism and excretion. Also, high bioavailability (≈100% was observed by s.c. administration. The blood contents following i.v. dose fits into a pharmacokinetic bi-compartmental model, consisting of high removal constants – kel 0.22 h−1 and kd 2.32 h−1and a half-life – t½ = 3.13 h. The Ames test of proteinase fraction (0.01–1% demonstrates absence of mutagenic activity. Likewise, genotoxic evaluation of proteinase fraction (5 or 10 mg/kg, i.p. shows no influence in micronuclei frequency. In conclusion, the acute doses for proteinase fraction lack mutagenic and genotoxic activity, clearing the way for clinical assays.

  15. Post-irradiation identification of papaya (Carica papaya L.) fruit

    International Nuclear Information System (INIS)

    Impact of radiation processing on the volatile essential oil profile of papaya (Carica papaya) was investigated. Gamma-radiation processing resulted in the appearance of a new peak in the GLC profile that was identified as phenol. The observed dose dependent increase in phenol content suggested possible use of this compound as a marker for radiation processed papaya. - Highlights: ► Effect of γ-irradiation on the essential oil profile of papaya is demonstrated. ► γ-Irradiation resulted in a dose dependent increase in a new peak, phenol. ► Phenol formed in the volatile oil is proposed as a new marker of irradiated food. ► Content of phenol remained unchanged during the entire storage period.

  16. TRANSGENIC PAPAYA: A CASE FOR MANAGING RISKS OF PAPAYA RINGSPOT VIRUS IN HAWAII

    Science.gov (United States)

    In May 1992, papaya ringspot virus (PRSV was detected in the Puna district of Hawaii Island, the main papaya growing region of the state of Hawaii. By 1994 Hawaii's papaya industry was facing devastating damage from PRSV. Efforts to develop resistant transgenic papaya were started in the mid 1980s...

  17. frutos de papaya

    Directory of Open Access Journals (Sweden)

    J. Adriana Sañudo Barajas

    2008-01-01

    Full Text Available En este estudio se determinaron los principales cambios en la composición de la pared celular y la degradación de pectinas solubles en agua relacionados con el ablandamiento postcosecha de frutos de papaya (Carica papaya L. cv. ‘Maradol’. Se estudiaron frutos en estado de madurez 1/2 (50-75 % de coloración amarillo-naranja en la cáscara, los cuales se retrasaron en la maduración con 300 nL L-1 de 1-metilciclopropeno (1-MCP o se adelantaron con 2.5 g L-1 de ácido 2-cloroetil fosfónico (etefón. Posterior a los tratamientos, los frutos se almacenaron en condiciones de simulación de mercadeo (20 ± 2 °C y HR 85 % durante 6 d. Se evaluaron los cambios en firmeza, composición de la pared celular y despolimerización de las pectinas solubles en agua. La aplicación de 1-MCP inhibió totalmente el ablandamiento de los frutos. Los frutos testigo perdieron 69 % de la firmeza a los 3 d de almacenamiento y los frutos con etefón perdieron 84 % en el mismo periodo. El ablandamiento en los frutos testigo y etefón se acompañó de la solubilización de ácidos urónicos y azúcares totales de las fracciones CDTA, Na2CO3, KOH 4% y KOH 24 %. La despolimerización extensiva de pectinas solubles en agua y la acumulación de oligómeros derivados de pectinas sólo fue detectada en los tratamientos testigo y etefón, lo que muestra una relación con el ablandamiento de los frutos y sugire la posible acción de la poligalacturonasa.

  18. chilena

    Directory of Open Access Journals (Sweden)

    David E. Hojman

    2005-01-01

    Full Text Available Chile es el único país latinoamericano donde las políticas económicas neoliberales han tenido éxito. Esto podría deberse tanto a la cultura nacional como a la cultura organizacional del país, que están estrechamente relacionadas. Ambas están llenas de contradicciones. El trabajo identifica algunas características más estables en el largo plazo de ambas culturas, así como también dramáticos cambios culturales ocurridos desde mediados de los años ochenta. Éstos últimos coexistieron con rápido crecimiento de la economía. Debido a ambigüedades, inconsistencias y contradicciones en la gestión organizacional, particularmente en lo relativo a recursos humanos, muchos empleados pueden haber sido forzados a adoptar patrones culturales y de comportamiento frecuentemente asociados a la caricatura Condorito

  19. Casa Chilena 1: Rancagua, Chile

    Directory of Open Access Journals (Sweden)

    Smiljan Radic

    2010-08-01

    Full Text Available Los nogales crecidos a los cuales se les ha dejado en paz, la negación del exterior gracias a una tapia perimetral pintada a la cal, un patio interior con posibilidades de ser cubierto por una sombra simple de membrana agrícola anclada a sus muros, un estar familiar austero con síntomas de exterior desplegado a todo lo largo del patio, un cielo de madera en bruto pintada y los dormitorios de colores fuertes, recuerdan de una u otra manera, las casas de inquilinos del campo chileno. En la Casa Chilena 2, se acentúa la negación del exterior y el patio interior se parte en dos generando exteriores matizados.The grown walnut trees left in peace, the negation of the exterior thanks to a whitewashed perimeter wall, an interior patio with the possibility of being covered by the simple shade of an agricultural screen anchored to its walls, an austere, family living room with exterior characteristics unfolding along the patio, a rough, painted wood ceiling and bedrooms in strong colors, remind one of the Inquilino houses of the Chilean countryside. In the Chilean House 2, the negation of the exterior is accentuated and the interior patio divides in two to generate blended exteriors.

  20. Complete Genome Sequence of Papaya Ringspot Virus Isolated from Genetically Modified Papaya in Hainan Island, China.

    Science.gov (United States)

    Zhao, Guangyuan; Yan, Pu; Shen, Wentao; Tuo, Decai; Li, Xiaoying; Zhou, Peng

    2015-01-01

    The complete genome sequence (10,326 nucleotides) of a papaya ringspot virus isolate infecting genetically modified papaya in Hainan Island of China was determined through reverse transcription (RT)-PCR. The virus shares 92% nucleotide sequence identity with the isolate that is unable to infect PRSV-resistant transgenic papaya. PMID:26358610

  1. Allergenicity assessment of the Papaya ringspot virus coat protein expressed in transgenic Rainbow papaya

    Science.gov (United States)

    The virus-resistant, transgenic commercial papaya cultivars Rainbow and SunUp (Carica papaya L.) have been consumed locally in Hawaii and elsewhere in the mainland US and Canada since their release to planters in Hawaii in 1998. These cultivars are derived from transgenic papaya line 55-1 and carry ...

  2. Managing papaya ringspot virus: Impact of grass barriers on alate aphid immigration into papaya orchards

    Science.gov (United States)

    Papaya ringspot virus, transmitted by alate aphids, is the most limiting factor of papaya production in the Caribbean region. Although there are transgenic papaya varieties that provide protection from this virus, these varieties are effective only in certain regions against certain strains of the v...

  3. Complete Genome Sequence of Papaya Ringspot Virus Isolated from Genetically Modified Papaya in Hainan Island, China

    OpenAIRE

    Zhao, Guangyuan; Yan, Pu; Shen, Wentao; Tuo, Decai; Li, Xiaoying; ZHOU, Peng

    2015-01-01

    The complete genome sequence (10,326 nucleotides) of a papaya ringspot virus isolate infecting genetically modified papaya in Hainan Island of China was determined through reverse transcription (RT)-PCR. The virus shares 92% nucleotide sequence identity with the isolate that is unable to infect PRSV-resistant transgenic papaya.

  4. Physical-chemical analyses of irradiated papayas (Carica papaya L.)

    Energy Technology Data Exchange (ETDEWEB)

    Camargo, R.J. [Radiation Technology Center, IPEN-CNEN/SP, Av. Lineu Prestes, 2242, 05508-900, Sao Paulo, SP (Brazil); Tadini, C.C. [Chemical Engineering Department, Escola Politecnica, Sao Paulo University, P.O. Box 61548, 05424-970, SP (Brazil); Sabato, S.F. [Radiation Technology Center, IPEN-CNEN/SP, Av. Lineu Prestes, 2242, 05508-900, Sao Paulo, SP (Brazil)], E-mail: sfsabato@ipen.br

    2007-11-15

    Papaya is cultivated in Espirito Santo State/Brazil and as it stands up to irradiation, it is important to validate this technology, since it is already being applied in some countries. Penetration energy, ratio (relation between soluble solids and titrable acidity) and skin color were evaluated to verify the influence of four different doses of irradiation (0.0, 0.5, 0.75 and 1.00 kGy) on papayas, during 21 days. As a result for the skin color and the penetration energy, it was found that in the first days after irradiation, these variables increased with increase in radiation dose; however, after a time lapse, the tendency inverted and the irradiated fruits had a slower ripening process. For the ratio, a very important variable that it is responsible for the fruit taste, no difference was found between irradiated and the control fruit. Color and texture measurements are dependent on the storage temperature.

  5. Physical-chemical analyses of irradiated papayas ( Carica papaya L.)

    Science.gov (United States)

    Camargo, R. J.; Tadini, C. C.; Sabato, S. F.

    2007-11-01

    Papaya is cultivated in Espírito Santo State/Brazil and as it stands up to irradiation, it is important to validate this technology, since it is already being applied in some countries. Penetration energy, ratio (relation between soluble solids and titrable acidity) and skin color were evaluated to verify the influence of four different doses of irradiation (0.0, 0.5, 0.75 and 1.00 kGy) on papayas, during 21 days. As a result for the skin color and the penetration energy, it was found that in the first days after irradiation, these variables increased with increase in radiation dose; however, after a time lapse, the tendency inverted and the irradiated fruits had a slower ripening process. For the ratio, a very important variable that it is responsible for the fruit taste, no difference was found between irradiated and the control fruit. Color and texture measurements are dependent on the storage temperature.

  6. Physical-chemical analyses of irradiated papayas (Carica papaya L.)

    International Nuclear Information System (INIS)

    Papaya is cultivated in Espirito Santo State/Brazil and as it stands up to irradiation, it is important to validate this technology, since it is already being applied in some countries. Penetration energy, ratio (relation between soluble solids and titrable acidity) and skin color were evaluated to verify the influence of four different doses of irradiation (0.0, 0.5, 0.75 and 1.00 kGy) on papayas, during 21 days. As a result for the skin color and the penetration energy, it was found that in the first days after irradiation, these variables increased with increase in radiation dose; however, after a time lapse, the tendency inverted and the irradiated fruits had a slower ripening process. For the ratio, a very important variable that it is responsible for the fruit taste, no difference was found between irradiated and the control fruit. Color and texture measurements are dependent on the storage temperature

  7. Somatic embryogenesis of Carica Papaya

    International Nuclear Information System (INIS)

    This paper describes the somatic embryogenesis of Carica papaya. Culture medium used was1/2 strength MS basal medium supplemented with 6% sucrose, 0.27 % agar, glutamine and various concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D). After 8 weeks in culture, the best concentration of 2,4-D to induce somatic embryo is at 45.2 μM. (Author)

  8. Gene Technology for Papaya Ringspot Virus Disease Management

    OpenAIRE

    Md. Abul Kalam Azad; Latifah Amin; Nik Marzuki Sidik

    2014-01-01

    Papaya (Carica papaya) is severely damaged by the papaya ringspot virus (PRSV). This review focuses on the development of PRSV resistant transgenic papaya through gene technology. The genetic diversity of PRSV depends upon geographical distribution and the influence of PRSV disease management on a sequence of PRSV isolates. The concept of pathogen-derived resistance has been employed for the development of transgenic papaya, using a coat protein-mediated, RNA-silencing mechanism and replicase...

  9. Literatura chilena de exilio, un vaco epistemolgico

    Directory of Open Access Journals (Sweden)

    Sol Marina Garay C

    2013-06-01

    Full Text Available Este artculo se presenta como una reflexin acerca del desinters actual que supone, dentro del panorama literario en Chile, el estudio de la literatura creada por autores y autoras chilenas durante el exilio chileno ocurrido entre 1973 y 1989. Una reflexin que espero sirva como referente para aquellos investigadores que se interesen por estudiar y dar a conocer los diversos corpus literarios vinculados al exilio chileno vivido en diferentes pases del mundo. Una produccin literaria an por desvelar y que agrupa un conjunto de corpus literarios que nos hablan del pasado reciente de Chile y sus habitantes, una literatura chilena de exilio en la cual las experiencias de dolor y desarraigo dan paso a la apertura al otro y al encuentro con diversas memorias culturales.

  10. Ocorrência de Ovulariopsis papayae Bijl em folhas de mamoeiro Occurrence of Ovulariopsis papayae Bijl in papaya leaves

    Directory of Open Access Journals (Sweden)

    Ivan José Antunes Ribeiro

    1988-01-01

    Full Text Available É citada pela primeira vez, em nossas condições, a ocorrência de oídio causado por Ovulariopsis papayae Bijl em folhas de mamoeiro (Cacica papaya L.. São descritas a sintomatologia, as características morfológicas do patógeno e o teste de patogenicidade.The occurrence causing powdery mildew in papaya leaves was observed for the first time in the State of São Paulo, Brazil. The symptomatology and the morphological characteristics of the pathogen were described and the pathogenicity was comproved.

  11. Genetic diversity studies of Papaya meleira virus

    Scientific Electronic Library Online (English)

    Cleidiane B., Daltro; Emanuel Felipe Medeiros, Abreu; Francisco Jose Lima, Arago; Eduardo C., Andrade.

    2014-02-01

    Full Text Available Papaya (Carica papaya) is a fruit crop of great economic and social importance for Brazil and other papaya-producing countries. Brazil is the second largest producer in the world. The papaya sticky disease, caused by Papaya meleira virus (PMeV), has caused great losses in the major Brazilian papaya- [...] producing states. In order to estimate the genetic diversity of PMeV, latex samples were collected from papaya plants in the states of Bahia, Esprito Santo, Pernambuco, Cear and Rio Grande do Norte, and total RNA was extracted. Specific primer for the replicase region allowed the amplification, by RT-PCR, of a fragment of approximately 560 bp from 31 isolates. The sequence analysis indicated a level of conservation greater than 88% among isolates. Furthermore, comparative analyzes indicated that PMeV has similarity with mycoviruses of the family Totiviridae. This phylogenetic relationship was reinforced by the presence of conserved motifs within in the RdRp regions from mycoviruses.

  12. HIGH SPEED CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY (HSCCC) ISOLATION AND IDENTIFICATION BY LC-MSn ANALYSIS OF THE POLAR PHENOLICS FROM VASCONCELLEA QUERCIFOLIA

    Scientific Electronic Library Online (English)

    CRISTINA, QUISPE; EZEQUIEL, VIVEROS-VALDEZ; JOS A, YARLEQUE; MARCO R, ARONES; JUAN C, PANIAGUA; GUILLERMO, SCHMEDA-HIRSCHMANN.

    2013-09-01

    Full Text Available High speed centrifugal countercurrent chromatography (HSCCC) was successfully applied to preparative isolation of the main polar phenolics from the Peruvian Caricaceae Vasconcellea quercifolia A. St.-Hil. High-performance liquid chromatography with diode array (HPLC-DAD) and electrospray ionisation [...] mass spectrometric detection (ESI-MS) was used to carry out a comprehensive characterisation of phenolic compounds from fruits and leaves of this plant. The main phenolics were the quercetin diglycoside rutin and the triglycoside manghaslin. Nine minor flavonoids were tentatively identified as kaempferol, quercetin, isorhamnetin and methoxymyricetin glycosides. HSCCC combined with other chromatographic methods allowed the isolation of the main phenolics from the plant. HPLC-DAD-MS analysis shows that the main phenolic compounds in V. quercifolia are similar to those described for V. pubescens but differs in the identity of the minor constituents.

  13. EFFECT OF UNRIPE CARICA PAPAYA ON UTERUS

    Directory of Open Access Journals (Sweden)

    Meera Sumanth

    2013-06-01

    Full Text Available The present study was under taken to establish the uterine stimulant activity of aqueous extract of unripe Carica papaya. The effect of the aqueous extract of unripe Carica papaya was studied on isolated rat uterus and embryo. Uterine stimulant activity was compared with clinically available drug oxytocin and the effect on embryo was compared with misoprostol. In the in vitro model, Carica papaya showed contractile effect on the isolated uterus in diestrus stage and when administered orally in dose of 200 and 400 mg/kg to pregnant rats, resulted in gradual decrease in body weight indicating embryonic resorption. The present study confirms the uterine stimulant activity of aqueous extract of unripe Carica papaya fruit.

  14. Improving Tenderness of Spent Layer Hens Meat Using Papaya Leaves (Carica papaya

    Directory of Open Access Journals (Sweden)

    H. O. Abdalla, N. N. A. Ali, F. S. Siddig and S. A. M. Ali*

    2013-01-01

    Full Text Available Two experiments were performed to study the use of papaya leaves as a meat tenderizer. The first experiment was to evaluate the effect of papaya dry leaves added to hen’s diet before slaughter. Spent hens (n=48 were used, half of them were fed a concentrate ration containing10% dried papaya leaves powder (DPLP while others received layer ration (Control, for 10 days. The second experiment involved a comparison between papaya leaves juice (PLJ, fresh papaya leaves (FPL and vinegar solution (VS as marinades applied to meat for one or two hours before cooking. Spent layer hens (n=42 were used for tenderness evaluation method. After slaughtering and preparing the chickens two methods of cooking were used (oven and moist cooking. The cooked parts (breast, thigh and drumstick were subjected to a panel test evaluation according to a designed questionnaire. Addition of dried papaya leaves powder to spent layer hens ration significantly (P≤0.05 increased the level of meat tenderness. Moist cooking had significantly (P≤0.05 improved meat tenderization compared to oven cooking. Meat treated with fresh papaya leaves had significantly (P≤0.05 improved tenderness. It was concluded that wrapping the tough meat of spent layer hens with fresh papaya leaves for one hour and moist cooking improve tenderness of meat.

  15. Validation and use of DNA markers for sex determination in papaya (Carica papaya)

    International Nuclear Information System (INIS)

    Profitable papaya production requires female and hermaphrodite plants in higher number than male plants. This is only possible if sex of plants is determined at an early growth stage. The present study was conducted to validate sex-linked DNA markers using plants from two Pakistani papaya varieties and subsequently utilize them for determination of sex in juvenile papaya plants. One hundred and five plants (including 49 male and 56 female) of two Pakistani Papaya varieties at flowering stage were screened with six DNA markers viz., W-11, T12, SDP, Napf-76Napf-76, PKBT4 and PKBT5. All male plants exhibited amplification of sex-linked alleles with markers T12 and W11, whereas, 96% and 95% of female plants showed the absence of sex-linked allele with these markers, respectively. Markers SDP, PKBT5 and Napf-76 showed the presence of sex-linked alleles in 98%, 96% and 93% of male plants, respectively, whereas the same markers showed the absence of sex-linked alleles in 100%, 96% and 94% of female plants. One marker, PKBT4 could not produce expected PCR amplification reported previously. The five DNA markers were further used to screen 171 papaya seedlings. These markers clearly differentiated male and female sex types in the studied papaya plants. Results of our study are likely to facilitate Pakistani papaya breeders and growers to incorporate DNA based screening at juvenile stage to determine sex at early stage and to ensure profitable papaya production. (author)

  16. Las franjas electorales en la experiencia chilena

    Directory of Open Access Journals (Sweden)

    Juan Ignacio García Rodríguez

    2013-07-01

    Full Text Available Expone la experiencia chilena sobre el establecimiento y regulación de las franjas electorales, para lo cual analiza la constitucionalidad de la franja, los principios involucrados, las disposiciones regulatorias, el papel del Consejo Nacional de Televisión, la jurisprudencia que sobre el tema ha sido emitida por el Tribunal Calificador de Elecciones y los retos que el sistema de franjas electorales enfrenta a futuro como lo es su aplicación a las elecciones primarias de los partidos políticos.

  17. Crecimiento, desarrollo y potencial productivo de la papaya (Carica papaya L.) variedad hawaina en el Caribe colombiano / Growth, development and the productive potential of the "Hawaiian" papaya variety (Carica papaya L.)

    OpenAIRE

    Martnez Bustamante, Enrique Guillermo

    1998-01-01

    En este trabajo se definen y cuantifican las diversas etapas de crecimiento y las variaciones entre los estados de desarrollo del rbol de papaya (Carica papaya L.) variedad Hawaiana y se conocen las condiciones para obtener crecimiento, desarrollo y rendimiento adecuado y su relacin con la produccin. / Abstract: Different growth and development variations among phases of the papaya tree (Carica papaya L.) are defined and quantified in this study, besides, both, development and yield are ...

  18. Transgenic Papaya: Can We Proceed Beyond the Hawaiian Experience?

    Science.gov (United States)

    The story of the development, deregulation, and commercialization of the papaya ringspot virus (PRSV) resistant transgenic SunUp and Rainbow papaya for Hawaii is quite well known at least among plant virologist and knowledgeable people in the field of papaya. Thus, the story will be only briefly r...

  19. 78 FR 48628 - Importation of Papayas From Peru

    Science.gov (United States)

    2013-08-09

    ... of Papayas From Peru AGENCY: Animal and Plant Health Inspection Service, USDA. ACTION: Proposed rule... of fresh papayas from Peru into the continental United States. The conditions for the importation of papayas from Peru would include requirements for approved production locations; field sanitation;...

  20. Correlation between ethylene emission and skin colour changes during papaya (Carica papaya L.) fruit ripening

    Science.gov (United States)

    da Silva, M. G.; Oliveira, J. G.; Vitoria, A. P.; Corrêa, S. F.; Pereira, M. G.; Campostrini, E.; Santos, E. O.; Cavalli, A.; Vargas, H.

    2005-06-01

    The skin colour changes and ethylene emission rates were monitored during papaya (C. papaya L.) fruit ripening. Two groups of papaya (‘Formosa’ and ‘Solo’) were applied in this study. The total colour difference was used as measured parameter and the corresponding half time of its saturation was used as correlation parameter. A high correlation factor between the saturation half time and corresponding climacteric peak time was found. It was concluded that high ethylene emission rate in ‘Solo’ fruit promotes a quick change of the total colour difference.

  1. Effect of gamma irradiation in papaya (Carica papaya L.) harvested in three degrees of maturation

    International Nuclear Information System (INIS)

    The papaya is a fragile tropical fruit with thin skin, susceptible to post harvest diseases and mechanical injuries. Furthermore, it is sensible to low temperatures and at normal conditions it ripens rapidly, increasing the difficulties with storage. The objective of this work was to evaluate the influence of gamma irradiation in papayas harvested in three degrees of maturation, in order to increase shelf life. To accomplish that, papayas were harvested in perfect quality conditions, washed, submitted to carnauba wax and selected by skin coloration into three distinct degrees of maturation: Mat 0, or beginning of yellow coloration; Mat 1, yellow stripes more developed, and Mat 2, one third yellow. Half of them were submitted to irradiation with 0, 75 kGy, while the others became control treatment. They were analyzed in four different periods of conservation, which were 1 day after irradiation (DAI) refrigerated (11 +- 1 deg C), 14 DAI refrigerated, 14 DAI refrigerated + 3 at room temperature (RT = 24 deg +- 2 deg C) and 14 DAI refrigerated + 6 at RT. The effect of irradiation was not influenced by the maturation degree at harvest. Irradiation promoted firmness maintenance of papaya and, therefore, delayed ripening; modified the green color of papaya to a lighter tone, which determined more homogeneity in the skin yellow color development (greater values of L* and b*), and turned the papaya flesh color lighter (rower values of b*). There was no effect of irradiation in papaya weight loss, in the occurrence of diseases, in the development of surface yellow color, in the parameter a* of papaya skin color, in the parameters L*, a* and chroma of flesh color, p H and total soluble solids content. Visual and organoleptic sensorial tests were accomplished with papayas from a new delivery in the conservation period of 14 DAI refrigerated + 3 at RT. In the visual test was evaluated the appearance of papaya in the following treatments: Mat O control, Mat O irradiated, Mat 1 control, Mat 1 irradiated, Mat 2 control e Mat 2 irradiated, in which the first one received lower scores due to the occurrence of ratting on one of the papayas. In the organoleptic test, the parameters firmness, sweetness and acceptance were evaluated for the following treatments: control, Mat O irradiated, Mat 1 irradiated e Mat 2 irradiated. There was no difference between treatments for the sweetness parameter. The irradiated treatments received better scores for firmness as well as papayas irradiated at Mat 1 and 2 for acceptance (author)

  2. Papaya Development Model As A Competitive Local Superior Commodity

    Directory of Open Access Journals (Sweden)

    Reny Sukmawani

    2014-12-01

    Full Text Available The aim of this research is to study the comparative advantage and papaya competitive and to design its development model by using the approach of local base agriculture development. This research uses survey method. The resulting research shows that papaya is a base commodity that has comparative advantage and competitive. The development papaya in the district of Sukabumi is quite good bases on eight superior creations. But in order to be the main sector in economic development and has a competition, the development of papaya must concern to its influence factors. In supporting papaya development as a competitive local superior commodity, it needs to be done some efforts are as follows: (1 increase a skillful worker; (2 improve business management; (3 increase papaya productivity by using technology and study papaya planted technology in specific local superior commodity; (4 develop the involvement of the business relation; (5 provide market information and information technology network; and (6 improve infrastructures.

  3. Free radical scavenging activity of papaya juice

    International Nuclear Information System (INIS)

    Papaya juice is an efficient scavenger of highly reactive hydroxyl radicals (OH radical) formed during 60Co irradiation of water. The OH anion radicals were detected by the electron spin resonance (ESR) technique of spin trapping using DMPO (5,5-dimethyl-1-pyrroline-N-oxide) or by a colorimetric assay in which salicylate is converted into polyhydroxybenzoic acids. Papaya juice is also able to quench the ESR signal of a stable free radical (TEMPOL) and the ESR signal of the DMPO-OH adduct. The active substance(s) in papaya juice are heat-stable, dialyzable, and soluble in water but not in lipid solvents. The active agents do not appear to be ascorbate, tocopherol, or carotenoids. (author)

  4. Quantification of the antiplasmodial alkaloid carpaine in papaya (Carica papaya) leaves.

    Science.gov (United States)

    Julianti, Tasqiah; Oufir, Mouhssin; Hamburger, Matthias

    2014-08-01

    Daily consumption of papaya (Carica papaya) leaves as greens and an herbal infusion is common in some parts of Indonesia as a means for preventing malaria. Antiplasmodial activity of the leaf extracts and of the main alkaloid carpaine were recently confirmed. A quantitative assay for determination of carpaine in papaya leaves was developed and validated. The assay involved pressurized liquid extraction and quantification with the aid of ultrahigh-performance liquid chromatography-tandem mass spectroscopy. Extraction conditions were optimized with respect to solvent, temperature, and number of extraction cycles. The ultrahigh-performance liquid chromatography-tandem mass spectroscopy assay was validated over a range of 20-5000 ng/mL (R(2) of 0.9908). A total of 29 papaya leaf samples were analyzed, and carpaine concentration in dry leaves was found to range from 0.02 to 0.31%. No obvious dependence on geographic origin and leaf maturity was observed. PMID:25153096

  5. Influence of ripening stages on antioxidant properties of papaya fruit (Carica papaya L.)

    Science.gov (United States)

    Addai, Zuhair Radhi; Abdullah, Aminah; Mutalib, Sahilah Abd.

    2013-11-01

    Papaya (Carica papaya L. cv Eksotika) is one of the most commonly consumed tropical fruits by humans, especially Malaysians. The objective of this study was to determine the phenolic compounds and antioxidants activity in different ripening stages of papaya fruit. The fruits were harvested at five different, stages RS1, RS2, RS3, RS4, and RS5 corresponding to 12, 14, 16, 18, and 20 weeks after anthesis, respectively. Papayas fruit at five different stage of ripening were obtained from farms at Pusat Flora Cheras, JabatanPertanian and Hulu Langat Semenyih, Selangor, Malaysia. The antioxidants activity were analyzed using the total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant Power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). The analyses were conducted in triplicate and the data were subjected to statistical analysis using SPSS. The results showed significant differences (Ppapaya fruits.

  6. Uptake of 32P labelled superphosphate by endomycorrhizal papaya (Carica papaya cv. Coorg honey dew)

    International Nuclear Information System (INIS)

    In papaya (Carica papaya cv. Coorg honey dew), there was an increase in 32P uptake and total phosphorus in plants inoculated with mycorrhizal fungi Glomus mossae and G. fasciculatum. Phosphorus derived from fertilizer (Pdff) was lower in mycorrhizal plants while soil derived P, utilisation of P and A values were higher showing thereby that mycorrhizal plants had utilised forms of phosphorus not available to non-mycorrhizal plants. (author). 13 refs., 1 tab

  7. Update on the development of virus-resistant papaya: Virus-resistant transgenic papaya for people in rural communities of Thailand

    Science.gov (United States)

    Papaya (Carica papaya L.) is one of the most important and preferred crops in rural communities in Thailand. Papaya ringspot virus (PRSV) is a serious disease of papaya throughout Thailand. Efforts to control the virus by various methods either have not been successful or have not resulted in sustai...

  8. The evolution of sex chromosomes in papaya

    Czech Academy of Sciences Publication Activity Database

    Yu, Qingyi; Moore, Paul H.; Alam, Maqsudul; Jiang, Jiming; Paterson, Andrew H.; Vyskot, Boris; Ming, Ray

    San Diego, 2006. W340-W340. [Plant & Animal Genomes XIV Conference. 14.01.2006-18.01.2006, San Diego] R&D Projects: GA ČR(CZ) GA521/06/0056; GA MŠk(CZ) LC06004 Institutional research plan: CEZ:AV0Z50040507 Keywords : evolution * sex chromosomes * papaya Subject RIV: BO - Biophysics

  9. Transgenic Papaya: Development, Release, Impact, and Challenges

    Science.gov (United States)

    Although the technology for developing virus-resistant transgenic plants through the use of the coat protein of a virus was unveiled twenty years ago, it is surprising to note that only a three virus-resistant plants (squash, potato, and papaya) have been commercialized in the U.S. The transgenic p...

  10. CARICA PAPAYA: A COMPLETE PACKAGE OF NUTRITIONALAND MEDICINAL BENEFITS

    Directory of Open Access Journals (Sweden)

    Karabhari Rekha Bhaskar

    2014-07-01

    Full Text Available Papaya (Carica papaya Linn. is well known for its exceptional nutritional and medicinal properties throughout the world. From the times immemorial, the whole Carica papaya plant including its leaves, seeds, ripe and unripe fruits and their juice is used as traditional medicine. The fruit has a large oval shape, yellowish -green skin and yellow flesh. Now a day, Carica papaya is considered as nutraceutical fruit due to its multi faceted medicinal and nutritional properties. The prominent medicinal properties of Carica papaya include anti-fertility, uterotonic, and diuretic, anti-hypertensive, wound healing, anti bacterial activities. Nutritionally the whole plant contains enzymes, vitamin A, vitamin C, B-complex vitamins and potassium. The present article reviews the nutritional and medicinal uses of Carica papaya.

  11. The draft genome of the transgenic tropical fruit tree papaya (Carica papaya Linnaeus)

    Science.gov (United States)

    Ming, Ray; Hou, Shaobin; Feng, Yun; Yu, Qingyi; Dionne-Laporte, Alexandre; Saw, Jimmy H.; Senin, Pavel; Wang, Wei; Ly, Benjamin V.; Lewis, Kanako L. T.; Salzberg, Steven L.; Feng, Lu; Jones, Meghan R.; Skelton, Rachel L.; Murray, Jan E.; Chen, Cuixia; Qian, Wubin; Shen, Junguo; Du, Peng; Eustice, Moriah; Tong, Eric; Tang, Haibao; Lyons, Eric; Paull, Robert E.; Michael, Todd P.; Wall, Kerr; Rice, Danny W.; Albert, Henrik; Wang, Ming-Li; Zhu, Yun J.; Schatz, Michael; Nagarajan, Niranjan; Acob, Ricelle A.; Guan, Peizhu; Blas, Andrea; Wai, Ching Man; Ackerman, Christine M.; Ren, Yan; Liu, Chao; Wang, Jianmei; Wang, Jianping; Na, Jong-Kuk; Shakirov, Eugene V.; Haas, Brian; Thimmapuram, Jyothi; Nelson, David; Wang, Xiyin; Bowers, John E.; Gschwend, Andrea R.; Delcher, Arthur L.; Singh, Ratnesh; Suzuki, Jon Y.; Tripathi, Savarni; Neupane, Kabi; Wei, Hairong; Irikura, Beth; Paidi, Maya; Jiang, Ning; Zhang, Wenli; Presting, Gernot; Windsor, Aaron; Navajas-Prez, Rafael; Torres, Manuel J.; Feltus, F. Alex; Porter, Brad; Li, Yingjun; Burroughs, A. Max; Luo, Ming-Cheng; Liu, Lei; Christopher, David A.; Mount, Stephen M.; Moore, Paul H.; Sugimura, Tak; Jiang, Jiming; Schuler, Mary A.; Friedman, Vikki; Mitchell-Olds, Thomas; Shippen, Dorothy E.; dePamphilis, Claude W.; Palmer, Jeffrey D.; Freeling, Michael; Paterson, Andrew H.; Gonsalves, Dennis; Wang, Lei; Alam, Maqsudul

    2010-01-01

    Papaya, a fruit crop cultivated in tropical and subtropical regions, is known for its nutritional benefits and medicinal applications. Here we report a 3 draft genome sequence of SunUp papaya, the first commercial virus-resistant transgenic fruit tree1 to be sequenced. The papaya genome is three times the size of the Arabidopsis genome, but contains fewer genes, including significantly fewer disease-resistance gene analogues. Comparison of the five sequenced genomes suggests a minimal angiosperm gene set of 13,311. A lack of recent genome duplication, atypical of other angiosperm genomes sequenced so far25, may account for the smaller papaya gene number in most functional groups. Nonetheless, striking amplifications in gene number within particular functional groups suggest roles in the evolution of tree-like habit, deposition and remobilization of starch reserves, attraction of seed dispersal agents, and adaptation to tropical daylengths. Transgenesis at three locations is closely associated with chloroplast insertions into the nuclear genome, and with topoisomerase I recognition sites. Papaya offers numerous advantages as a system for fruit-tree functional genomics, and this draft genome sequence provides the foundation for revealing the basis of Carica's distinguishing morpho-physiological, medicinal and nutritional properties. PMID:18432245

  12. Physical Characteristics of the Leaves and Latex of Papaya Plants Infected with the Papaya meleira Virus

    Science.gov (United States)

    Magaña-Álvarez, Anuar; Vencioneck Dutra, Jean Carlos; Carneiro, Tarcio; Pérez-Brito, Daisy; Tapia-Tussell, Raúl; Ventura, Jose Aires; Higuera-Ciapara, Inocencio; Fernandes, Patricia Machado Bueno; Fernandes, Antonio Alberto Ribeiro

    2016-01-01

    Sticky disease, which is caused by Papaya meleira virus (PMeV), is a significant papaya disease in Brazil and Mexico, where it has caused severe economic losses, and it seems to have spread to Central and South America. Studies assessing the pathogen-host interaction at the nano-histological level are needed to better understand the mechanisms that underlie natural resistance. In this study, the topography and mechanical properties of the leaf midribs and latex of healthy and PMeV-infected papaya plants were observed by atomic force microscopy and scanning electron microscopy. Healthy plants displayed a smooth surface with practically no roughness of the leaf midribs and the latex and a higher adhesion force than infected plants. PMeV promotes changes in the leaf midribs and latex, making them more fragile and susceptible to breakage. These changes, which are associated with increased water uptake and internal pressure in laticifers, causes cell disruption that leads to spontaneous exudation of the latex and facilitates the spread of PMeV to other laticifers. These results provide new insights into the papaya-PMeV interaction that could be helpful for controlling papaya sticky disease. PMID:27092495

  13. COMPARATIVE STUDIES ON ANTHELMINTIC POTENTIAL OF CUCURBITA MAXIMA (PUMPKIN SEEDS AND CARICA PAPAYA (PAPAYA SEEDS

    Directory of Open Access Journals (Sweden)

    Sengupta Rupa

    2013-08-01

    Full Text Available The crude extract of Carica papaya (papaya seeds (CP and Cucurbita maxima (Pumpkin seeds (CM were assayed against adult earthworms (Pheretima posthuma for the evaluation of anthelmintic activity. Various concentrations of both extracts were tested and results were expressed in terms of time for paralysis (P and time for death (D of worms. Albendazole was used as a reference standard. The result showed that in both of the extracts (i.e. CP and CM dose of 60 mg / ml possesses more wormicidal activity. The time of paralysis was 1.88 ± 0.52 minute and 1.93 ± 0.57 minute whereas the time of death was 3.45 ± 0.17 minute and 4.90 ± 0.18 minute in the case of Carica papaya and Cucurbita maxima respectively. In conclusion, the use of seeds of Carica papaya (CP and Cucurbita maxima (CM for anthelmintic activity have been confirmed and further studies are suggested to isolate the active principles responsible for the activity. Both the extracts showed significant anthelmintic activity, but the comparative study showed that out of these two, Carica papaya proves to be a better anthelmintic remedy.

  14. Physical Characteristics of the Leaves and Latex of Papaya Plants Infected with the Papaya meleira Virus.

    Science.gov (United States)

    Magaña-Álvarez, Anuar; Vencioneck Dutra, Jean Carlos; Carneiro, Tarcio; Pérez-Brito, Daisy; Tapia-Tussell, Raúl; Ventura, Jose Aires; Higuera-Ciapara, Inocencio; Fernandes, Patricia Machado Bueno; Fernandes, Antonio Alberto Ribeiro

    2016-01-01

    Sticky disease, which is caused by Papaya meleira virus (PMeV), is a significant papaya disease in Brazil and Mexico, where it has caused severe economic losses, and it seems to have spread to Central and South America. Studies assessing the pathogen-host interaction at the nano-histological level are needed to better understand the mechanisms that underlie natural resistance. In this study, the topography and mechanical properties of the leaf midribs and latex of healthy and PMeV-infected papaya plants were observed by atomic force microscopy and scanning electron microscopy. Healthy plants displayed a smooth surface with practically no roughness of the leaf midribs and the latex and a higher adhesion force than infected plants. PMeV promotes changes in the leaf midribs and latex, making them more fragile and susceptible to breakage. These changes, which are associated with increased water uptake and internal pressure in laticifers, causes cell disruption that leads to spontaneous exudation of the latex and facilitates the spread of PMeV to other laticifers. These results provide new insights into the papaya-PMeV interaction that could be helpful for controlling papaya sticky disease. PMID:27092495

  15. The draft genome of the transgenic tropical fruit tree papaya (Carica papaya Linnaeus).

    Science.gov (United States)

    Ming, Ray; Hou, Shaobin; Feng, Yun; Yu, Qingyi; Dionne-Laporte, Alexandre; Saw, Jimmy H; Senin, Pavel; Wang, Wei; Ly, Benjamin V; Lewis, Kanako L T; Salzberg, Steven L; Feng, Lu; Jones, Meghan R; Skelton, Rachel L; Murray, Jan E; Chen, Cuixia; Qian, Wubin; Shen, Junguo; Du, Peng; Eustice, Moriah; Tong, Eric; Tang, Haibao; Lyons, Eric; Paull, Robert E; Michael, Todd P; Wall, Kerr; Rice, Danny W; Albert, Henrik; Wang, Ming-Li; Zhu, Yun J; Schatz, Michael; Nagarajan, Niranjan; Acob, Ricelle A; Guan, Peizhu; Blas, Andrea; Wai, Ching Man; Ackerman, Christine M; Ren, Yan; Liu, Chao; Wang, Jianmei; Wang, Jianping; Na, Jong-Kuk; Shakirov, Eugene V; Haas, Brian; Thimmapuram, Jyothi; Nelson, David; Wang, Xiyin; Bowers, John E; Gschwend, Andrea R; Delcher, Arthur L; Singh, Ratnesh; Suzuki, Jon Y; Tripathi, Savarni; Neupane, Kabi; Wei, Hairong; Irikura, Beth; Paidi, Maya; Jiang, Ning; Zhang, Wenli; Presting, Gernot; Windsor, Aaron; Navajas-Pérez, Rafael; Torres, Manuel J; Feltus, F Alex; Porter, Brad; Li, Yingjun; Burroughs, A Max; Luo, Ming-Cheng; Liu, Lei; Christopher, David A; Mount, Stephen M; Moore, Paul H; Sugimura, Tak; Jiang, Jiming; Schuler, Mary A; Friedman, Vikki; Mitchell-Olds, Thomas; Shippen, Dorothy E; dePamphilis, Claude W; Palmer, Jeffrey D; Freeling, Michael; Paterson, Andrew H; Gonsalves, Dennis; Wang, Lei; Alam, Maqsudul

    2008-04-24

    Papaya, a fruit crop cultivated in tropical and subtropical regions, is known for its nutritional benefits and medicinal applications. Here we report a 3x draft genome sequence of 'SunUp' papaya, the first commercial virus-resistant transgenic fruit tree to be sequenced. The papaya genome is three times the size of the Arabidopsis genome, but contains fewer genes, including significantly fewer disease-resistance gene analogues. Comparison of the five sequenced genomes suggests a minimal angiosperm gene set of 13,311. A lack of recent genome duplication, atypical of other angiosperm genomes sequenced so far, may account for the smaller papaya gene number in most functional groups. Nonetheless, striking amplifications in gene number within particular functional groups suggest roles in the evolution of tree-like habit, deposition and remobilization of starch reserves, attraction of seed dispersal agents, and adaptation to tropical daylengths. Transgenesis at three locations is closely associated with chloroplast insertions into the nuclear genome, and with topoisomerase I recognition sites. Papaya offers numerous advantages as a system for fruit-tree functional genomics, and this draft genome sequence provides the foundation for revealing the basis of Carica's distinguishing morpho-physiological, medicinal and nutritional properties. PMID:18432245

  16. La izquierda chilena contemparánea

    Directory of Open Access Journals (Sweden)

    Manuel Antonio Garretón

    2006-01-01

    Full Text Available En este artículo, el autor examina las particularidades de la izquierda chilena para entender su papel en los gobiernos democráticos desde los años 70. El autor parte de la premisa de la existencia, durante los años 90, de dos izquierdas: una, el socialismo dentro de la coalición; la otra, el comunismo fuera de ella. En el trabajo se sostiene que la izquierda socialista ha sido el principal motor de la coalición y su papel específico se ha concentrado en los temas socio-económicos ligados a la igualdad, a la justicia, a los derechos humanos y a las propuestas culturales de tipo progresista. Asimismo, se analizan las particularidades esenciales de los gobiernos democráticos chilenos y se examinan los temas y las tareas pendientes. El artículo concluye con un balance del papel de la izquierda y de los retos que aún tiene por delante.

  17. Fusarium species associated with fruit rot of banana (Musa spp.), papaya (Carica papaya) and guava (Psidium guajava)

    OpenAIRE

    L. Zakaria; Mazzura, W. C.; Kong, W. H.; S. Baharuddin

    2012-01-01

    A total of 60 isolates of Fusarium were isolated from fruit rot of banana (Musa spp.), papaya (Carica papaya) and guava(Psidium guajava). The most common species recovered from the fruit rot of the three fruit crops were F. semitectum(40 %), F. solani (38.3 %), F. verticillioides (11.7 %) and F. oxysporum (10 %). Fusarium semitectum was isolated from fruit rot of banana, papaya and guava; F. oxysporum from banana and papaya; F. solani from banana and guava and F.verticillioides from banana. F...

  18. Extraction and characteristics of seed oil from Papaya (Carica papaya in Congo-Brazzaville

    Directory of Open Access Journals (Sweden)

    G. Bouanga-Kalou

    2011-03-01

    Full Text Available Papaya seeds were collected and dried. This study was carried out on papaya seed to clarify their proximate composition and the characteristics of the extracted oil including unsaponifiable matter and fatty acid composition. The seed is a rich source of protein (26.78% and crude fiber (21.4%. Palmitic acid was the main saturated fatty acid (15.22%, while linoleic acid was the major unsaturated fatty acid (76.38% in all lipid classes. The physical properties of the oil extracts showed the state to be liquid at room temperature. Carica papaya seeds have ash content of 3.2% (with the presence of following minerals: K, Na, Ca, P and Mg. However, Ca and P occur in appreciable quantities (18212.12 mg/100 g dry matter and 11561.8 mg/100 g dry mater, respectively.

  19. Antioxidant Activity and Physicochemical Properties of Mature Papaya Fruit (Carica papaya L. cv. Eksotika

    Directory of Open Access Journals (Sweden)

    Zuhair Radhi Addai

    2013-07-01

    Full Text Available Papaya (Carica papaya L. cv. Eksotika is one of the most commonly consumed tropical fruits by humans, especially Malaysians. The objective of this study was to evaluate the effect of the maturity stage (12, 14, 16, 18 and 20 weeks after anthesis of papaya fruit on its physicochemical properties, antioxidant capacity and sensory characteristics. Papaya fruits were selected and classified based on their visual maturity, i.e., stages 1 to 5. The activities of several antioxidants were tested, including the Total Phenolic Content (TPC, Total Flavonoid Content (TFC, Ferric Reducing Antioxidant Power (FRAP, 2,2-diphenyl-1-picrylhydrazyl (DPPH and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS. The physicochemical changes were measured in terms of the pH, Titratable Acidity (TA, moisture, Total Soluble Solids (TSS and pulp color of the papaya fruits at the five ripening stages. Significant differences (p<0.05 were found in different degrees of ripening. The pH of the fruit decreased significantly (p<0.05, whereas the TA, moisture and TSS all increased significantly (p<0.05 with maturity. The redness (a* and yellowness (b* values of the fruit color both increased significantly, whereas the lightness (L* of the color fluctuated. The TPC, TFC, FRAP, DPPH and ABTS values also increased significantly (p<0.05 with ripening. Sensory evaluation based on the color, sweetness, sourness, flavor and overall acceptance for the last three maturity stages was also performed. Stage 5 had a better score than stages 3 or 4. The results showed the important role of the ripening stage in increasing the antioxidant content of papaya fruits.

  20. Virus Resistant Transgenic Papaya: Commercial Development and Regulatory and Environmental Issues

    Science.gov (United States)

    In Hawaii, transgenic papaya resistant to Papaya ringspot virus (PRSV) was developed starting in the 1980s and released commercially in 1998 to combat the wide spread destruction of Hawaii’s papaya industry. This review describes the proactive development of the transgenic papaya and its impact on ...

  1. Whole-photosynthesis and transpiration in field-grown papaya plants

    Science.gov (United States)

    Papaya (Carica papaya L.) is a principal horticultural crop of tropical and subtropical regions. Knowledge of papaya response to environmental factors provides a scientific basis to develop management strategies to optimize fruit yield and quality. In papaya, the photosynthetic capacity also influ...

  2. 7 CFR 319.56-25 - Papayas from Central America and Brazil.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 5 2010-01-01 2010-01-01 false Papayas from Central America and Brazil. 319.56-25... § 319.56-25 Papayas from Central America and Brazil. The Solo type of papaya may be imported into the... section and all other applicable provisions of this subpart. (a) The papayas were grown and packed...

  3. Carica papaya MicroRNAs Are Responsive to Papaya meleira virus Infection

    Science.gov (United States)

    Abreu, Paolla M. V.; Gaspar, Clicia G.; Buss, David S.; Ventura, José A.; Ferreira, Paulo C. G.; Fernandes, Patricia M. B.

    2014-01-01

    MicroRNAs are implicated in the response to biotic stresses. Papaya meleira virus (PMeV) is the causal agent of sticky disease, a commercially important pathology in papaya for which there are currently no resistant varieties. PMeV has a number of unusual features, such as residence in the laticifers of infected plants, and the response of the papaya to PMeV infection is not well understood. The protein levels of 20S proteasome subunits increase during PMeV infection, suggesting that proteolysis could be an important aspect of the plant defense response mechanism. To date, 10,598 plant microRNAs have been identified in the Plant miRNAs Database, but only two, miR162 and miR403, are from papaya. In this study, known plant microRNA sequences were used to search for potential microRNAs in the papaya genome. A total of 462 microRNAs, representing 72 microRNA families, were identified. The expression of 11 microRNAs, whose targets are involved in 20S and 26S proteasomal degradation and in other stress response pathways, was compared by real-time PCR in healthy and infected papaya leaf tissue. We found that the expression of miRNAs involved in proteasomal degradation increased in response to very low levels of PMeV titre and decreased as the viral titre increased. In contrast, miRNAs implicated in the plant response to biotic stress decreased their expression at very low level of PMeV and increased at high PMeV levels. Corroborating with this results, analysed target genes for this miRNAs had their expression modulated in a dependent manner. This study represents a comprehensive identification of conserved miRNAs inpapaya. The data presented here might help to complement the available molecular and genomic tools for the study of papaya. The differential expression of some miRNAs and identifying their target genes will be helpful for understanding the regulation and interaction of PMeV and papaya. PMID:25072834

  4. Extraction and characteristics of seed oil from Papaya (Carica papaya) in Congo-Brazzaville

    OpenAIRE

    G. Bouanga-Kalou; A. Kimbonguila; J.M. Nzikou; F.B. Ganongo-Po; F.E. Moutoula; E. Panyoo-Akdowa; Th. Silou and S. Desobry

    2011-01-01

    Papaya seeds were collected and dried. This study was carried out on papaya seed to clarify their proximate composition and the characteristics of the extracted oil including unsaponifiable matter and fatty acid composition. The seed is a rich source of protein (26.78%) and crude fiber (21.4%). Palmitic acid was the main saturated fatty acid (15.22%), while linoleic acid was the major unsaturated fatty acid (76.38%) in all lipid classes. The physical properties of the oil extracts showed the ...

  5. Influence of gamma radiation on carbohydrates metabolism of ripening papaya (Carica papaya L. cv. Solo)

    International Nuclear Information System (INIS)

    Food irradiation is one of the most promising treatments that can be utilized for fruits disinfestation and extension of shelf life. The authors studied the influence of 0,5 kGy of Gamma irradiation on the soluble carbohydrates composition of papaya (Carica papaya L. cv. Solo) fruit, and on sucrose metabolizing enzymes: sucrose synthase (SS), sucrose-phosphate synthase, acid and neutral invertases activities, during ripening. The results demonstrated that ethylene production, total soluble sugars, sucrose content, and sucrose-phosphate synthase and invertases activities were affected by irradiation, but not respiration, glucose and fructose content, and SS activity. (author)

  6. Dwarf mutant of papaya (Carica papaya L.) induced by gamma rays

    International Nuclear Information System (INIS)

    Treating the seeds of papaya with 15 Krad of gamma rays, three plants were isolated from the M3 population. Repeated full sibmating among the progenies of these dwarf mutants helped in establishing a homozygous population of dwarf papaya in M6 generation. Though the per plant yield in mutant is less, nevertheless its importance is envisaged in the fact that it is likely to incur less damage by wind and storm, can be better managed and more number of plants can be accomodated per unit area, thus giving increased yield per hectare. (author)

  7. GENETIC DIVERSITY OF CARICA PAPAYA AS REVEALED BY AFLP MARKERS

    Science.gov (United States)

    Genetic relationships among Carica papaya cultivars, breeding lines, unimproved germplasm, and related species were established using amplified fragment length polymorphism (AFLP) markers. Seventy-one papaya accessions and related species were analyzed with nine EcoRI-MseI primer combinations. A t...

  8. Gamma radiation in papaya harvested at three stages of maturation

    International Nuclear Information System (INIS)

    Papaya is a fragile, perishable fruit, highly accepted worldwide. To keep the quality of papaya from harvest to the consumers, conservation techniques are often used; among them is the application of gamma irradiation. The objective of this work was to evaluate gamma irradiation in papayas harvested at three degrees of maturation, in order to increase shelf life. Papayas were harvested in perfect quality conditions and selected by skin coloration into three distinct degrees of maturation: maturation 0, or beginning of yellow coloration; maturation 1, yellow stripes more developed, and maturation 2, one third yellow. Half of them were irradiated with 0.75 kGy, while the other half became control treatment. They were analyzed in four periods of conservation, which were 1 DAI (days after irradiation refrigerated at 11 ± 1 deg C), 14 DAI, 14 DAI + 3 DRT (room temperature at 24 deg C ± 2 deg C) and 14 DAI + 6 DRT. The papaya maturation degree at harvest did not influence the radiation effect. Irradiation maintained firmness of papaya and, therefore, delayed ripening; modified the green color of papaya to a lighter, more intense tone, which determined more homogeneity in the development of the skin's yellow color (greater values of L* and chroma). There was no effect of irradiation in papaya weight loss, occurrence of diseases, chroma of flesh color, p H and total soluble solids contents. (author)

  9. Papaya Ringspot Virus: Characteristics, Pathogenicity, Sequence Variability and Control

    Science.gov (United States)

    Taxonomy: Papaya ringspot virus (PRSV) is an aphid-transmitted plant virus belonging to the genus Potyvirus of the family Potyviridae with a positive sense RNA genome. PRSV isolates belong to either one of two major strains, P-type or W-type. The P-type infects both papaya and cucurbits whereas th...

  10. Gamma radiation in papaya harvested at three stages of maturation

    Energy Technology Data Exchange (ETDEWEB)

    Pimentel, Rodrigo Meirelles de Azevedo; Walder, Julio Marcos Melges [Centro de Energia Nuclear na Agricultura, Piracicaba, SP (Brazil). Lab. de Irradiacao de Alimentos]. E-mail: jmwalder@cena.usp.br

    2004-04-01

    Papaya is a fragile, perishable fruit, highly accepted worldwide. To keep the quality of papaya from harvest to the consumers, conservation techniques are often used; among them is the application of gamma irradiation. The objective of this work was to evaluate gamma irradiation in papayas harvested at three degrees of maturation, in order to increase shelf life. Papayas were harvested in perfect quality conditions and selected by skin coloration into three distinct degrees of maturation: maturation 0, or beginning of yellow coloration; maturation 1, yellow stripes more developed, and maturation 2, one third yellow. Half of them were irradiated with 0.75 kGy, while the other half became control treatment. They were analyzed in four periods of conservation, which were 1 DAI (days after irradiation refrigerated at 11 {+-} 1 deg C), 14 DAI, 14 DAI + 3 DRT (room temperature at 24 deg C {+-} 2 deg C) and 14 DAI + 6 DRT. The papaya maturation degree at harvest did not influence the radiation effect. Irradiation maintained firmness of papaya and, therefore, delayed ripening; modified the green color of papaya to a lighter, more intense tone, which determined more homogeneity in the development of the skin's yellow color (greater values of L{sup *} and chroma). There was no effect of irradiation in papaya weight loss, occurrence of diseases, chroma of flesh color, p H and total soluble solids contents. (author)

  11. 75 FR 22207 - Importation of Papayas From Colombia and Ecuador

    Science.gov (United States)

    2010-04-28

    ... published in the Federal Register (74 FR 18161-18166, Docket No. APHIS-2008-0050) a proposal\\1\\ to amend the... Animal and Plant Health Inspection Service 7 CFR Part 319 RIN 0579-AC95 Importation of Papayas From... importation of commercial shipments of fresh papayas from Colombia and Ecuador into the continental...

  12. 76 FR 13972 - Notice of Availability of a Pest Risk Analysis for the Importation of Fresh Papaya Fruit From...

    Science.gov (United States)

    2011-03-15

    ... Importation of Fresh Papaya Fruit From Malaysia Into the Continental United States AGENCY: Animal and Plant... of fresh papaya (Carica papaya) fruit from Malaysia into the continental United States. Based on our... edible fresh fruit of papaya (Carica papaya) into the continental United States. Currently, fresh...

  13. EVALUACIN DE LA CALIDAD Y EL RENDIMIENTO EN PAPAYA SILVESTRE (Carica papaya L. DE CUBA

    Directory of Open Access Journals (Sweden)

    Jess Rodrguez Cabello

    2014-01-01

    Full Text Available La papaya (Carica papaya L. de la familia Caricaceae, es nativa del trpico americano y la especie ms importante del gnero Carica por su alto valor nutritivo e industrial. En sus reas de origen existen parientes silvestres que proporcionan alimento a las comunidades que las mantienen y de ser aprovechadas, podran constituir una importante fuente de ingresos debido a que poseen genes tiles para elevar el rendimiento y la calidad nutricional de sus frutos. Existe referencia de las poblaciones de papaya silvestres en nuestro pas. Sin embargo, an no se han caracterizado o evaluado con profundidad, de modo que se desconoce el rendimiento y la calidad de sus frutos. El presente estudio se llev a cabo con el propsito de evaluar genotipos de papaya silvestre, prospectados en las alturas del nacimiento de las Cuencas Almendares-Vento y Noreste de la Habana, a travs de indicadores fsicos, qumicos y el rendimiento, que permitan valorar su aceptacin en el mercado. Los resultados mostraron alto rendimiento y frutos con caractersticas que les permiten ser de gran aceptacin en el mercado para consumo en fresco por su masa, forma, dimensiones, slidos solubles totales y color de la cscara y la pulpa. Adems, las caractersticas apreciadas favorecen su empleo para futuros programas de mejoramiento gentico en Cuba, con el propsito de obtener cultivares con alto nmero de frutos de mediano a pequeo tamao por planta.|

  14. Effects of heat, irradiation and their combination on the keeping quality of papaya (carica papaya linn.)

    International Nuclear Information System (INIS)

    An experimental work has been done to determine the effect of gamma irradiation either alone or in combination with hot water dipping on the shelf life of fresh papaya. Mature green papayas of 'semangka paris' variety were treated under 3 different conditions, i.e. dipped in hot water at 50 Celsius degrees for 10 minutes; irradiated in air with a dose of 0.5 kGy; treated with combination of hot water dipping at 50 Celsius degrees for 10 minutes and irradiation with a dose of 0.5 kGy, and stored at ambient conditions. Quality evaluation was done subjectively during storage on the colour, texture, and taste. Besides, weight loss was also observed. Chemical characteristics, namely the contents of vitamin C, β-carotene, total soluble pectin, and reducing sugar were also determined as objective parameters. The results revealed that ripening of mature green papaya could be delayed by irradiation with the dose of 0.5 kGy. Best result was obtained from the combination treatment. The treated papayas were still acceptable up to 8 days of storage, whereas the untreated ones were acceptable only up to 5 days of storage. (author)

  15. Effect of Biopreservatives on Storage Life of Papaya (Carica papaya L.

    Directory of Open Access Journals (Sweden)

    Fatema H. Brishti

    2013-04-01

    Full Text Available Normal 0 false false false EN-US X-NONE X-NONE In this experiment the effect on post-harvest preservation of papaya (Carica papaya L. fruit coated with either Aloe gel (AG; 100% or papaya leaf extract with Aloe gel (PLEAG; 1:1 was studied. To evaluate the role of coating on ripening behavior and quality of papaya the uncoated and coated fruits were stored and ripened at room temperature (25 °C-29 °C and 82-84% relative humidity. Physico-chemical properties were analyzed at 4 day intervals during the storage period. The incidence of disease attack was also visually observed. The overall results showed the superiority of AG and PLEAG coating in lengthening the shelf-life of papaya fruit compared to controls which showed significant decay from 6th day onward and complete decay within 12 days of storage. The AG and PLEAG coated fruits maintained their shelf life for 12 days and decayed at 16th day. The coated fruits also maintained their color, flavor and firmness up to 12 days of storage. An increase in ascorbic acid content (120.2 mg/100 g was also found in coated fruits in contrast to the control (59 mg/100 g. Only 27% disease incidence was observed in AG and 13% in PLEAG coated fruits as compared to control (100% during the storage period. The results of this study show that both AG and PLEAG coatings have excellent potential to be used on fresh produce to maintain quality and extend shelf-life.

  16. Origin and domestication of papaya Yh chromosome

    OpenAIRE

    VanBuren, Robert; Zeng, Fanchang; Chen, Cuixia; Zhang, Jisen; Wai, Ching Man; Han, Jennifer; Aryal, Rishi; Gschwend, Andrea R; Wang, Jianping; Na, Jong-Kuk; Huang, Lixian; Zhang, Lingmao; Miao, Wenjing; Gou, Jiqing; Arro, Jie

    2015-01-01

    Sex in papaya is controlled by a pair of nascent sex chromosomes. Females are XX, and two slightly different Y chromosomes distinguish males (XY) and hermaphrodites (XYh). The hermaphrodite-specific region of the Yh chromosome (HSY) and its X chromosome counterpart were sequenced and analyzed previously. We now report the sequence of the entire male-specific region of the Y (MSY). We used a BAC-by-BAC approach to sequence the MSY and resequence the Y regions of 24 wild males and the Yh region...

  17. Radiation-induced mutation breeding of papaya

    International Nuclear Information System (INIS)

    Irradiation-induced mutation breeding of papaya commenced at the Malaysian Agricultural Research and Development Institute (MARDI) in August, 2000. This research was initiated under a Coordinated Research Project (CRP - D23023) with assistance from the International Atomic Energy Agency (IAEA). In the preliminary dosimetry study, seeds from two local papaya varieties, Sekaki and Eksotika were irradiated, either as dry seeds or as pre-soaked seeds (soaked overnight in water and surface-dried) with radiation doses ranging from 0 to 300 Gy. 100 Gy dose was lethal for all wet presoaked seeds while dry seed did not show loss of viability, even at 300 Gy. From the growth data it was estimated that dose of 525 Gy reduced shoot elongation by 50%, and this dose was recommended for mass irradiation of dry seeds. For wet, pre-soaked seeds results indicated that 42.5 Gy was the optimal dose for mass irradiation. At this dose, both seeds germination and seedlings growth were reduced by 50%. In a massive irradiation experiment 2,000 Eksotika seeds were irradiated at 42.5 Gy (pre-soaked) and another 2,000 at 525 Gy (dry). In the M2 population, numerous physiological defects were observed, including stem splitting, leaf variegation and puckering, and crinkled dwarfs. In the M3 population, a wide variability was recorded for a number of traits. M3 seedlings derived from presoaked seeds irradiated a low 42.5 Gy dose presented a high number of plants that were shorter and more vigorous in leaf development compared to those irradiated at 525 Gy and to non-irradiated control seedlings. The distribution patterns of M3 progenies for nine quantitative field characters showed great variation, often exceeding the limits of the control population. There appears to be good prospects in improving Eksotika papaya especially in the development of dwarf trees with lower fruit bearing stature, higher total soluble solids in fruits and larger fruit size. Several M2 and M3 putative mutants also showed very good resistance to malformed top disease. However, no resistance to papaya ringspot virus disease was found in the 1920 M2 seedlings that were inoculated. (author)

  18. Whole genome sequence analysis of unidentified genetically modified papaya for development of a specific detection method.

    Science.gov (United States)

    Nakamura, Kosuke; Kondo, Kazunari; Akiyama, Hiroshi; Ishigaki, Takumi; Noguchi, Akio; Katsumata, Hiroshi; Takasaki, Kazuto; Futo, Satoshi; Sakata, Kozue; Fukuda, Nozomi; Mano, Junichi; Kitta, Kazumi; Tanaka, Hidenori; Akashi, Ryo; Nishimaki-Mogami, Tomoko

    2016-08-15

    Identification of transgenic sequences in an unknown genetically modified (GM) papaya (Carica papaya L.) by whole genome sequence analysis was demonstrated. Whole genome sequence data were generated for a GM-positive fresh papaya fruit commodity detected in monitoring using real-time polymerase chain reaction (PCR). The sequences obtained were mapped against an open database for papaya genome sequence. Transgenic construct- and event-specific sequences were identified as a GM papaya developed to resist infection from a Papaya ringspot virus. Based on the transgenic sequences, a specific real-time PCR detection method for GM papaya applicable to various food commodities was developed. Whole genome sequence analysis enabled identifying unknown transgenic construct- and event-specific sequences in GM papaya and development of a reliable method for detecting them in papaya food commodities. PMID:27006240

  19. Antioxidant Activity of Papaya Seed Extracts

    Directory of Open Access Journals (Sweden)

    Haofu Dai

    2011-07-01

    Full Text Available The antioxidant activities of the ethanol, petroleum ether, ethyl acetate, n-butanol and water extract fractions from the seeds of papaya were evaluated in this study. The ethyl acetate fraction showed the strongest DPPH and hydroxyl free radical-scavenging activities, and its activities were stronger than those of ascorbic acid and sodium benzoate, respectively. The n-butanol fraction demonstrated the greatest ABTS+ radicals scavenging activity. The ethyl acetate fraction and the n-butanol fraction not only showed higher antioxidant activities than the petroleum ether fraction, water fraction and ethanol fraction, but also showed higher superoxide anion and hydrogen peroxide radicals scavenging activities than those of the other extract fractions. The high amount of total phenolics and total flavonoids in the ethyl acetate and n-butanol fractions contributed to their antioxidant activities. The ethyl acetate fraction was subjected to column chromatography, to yield two phenolic compounds, p-hydroxybenzoic acid (1 and vanillic acid (2, which possessed significant antioxidant activities. Therefore, the seeds of papaya and these compounds might be used as natural antioxidants.

  20. Antioxidant activity of papaya seed extracts.

    Science.gov (United States)

    Zhou, Kaibing; Wang, Hui; Mei, Wenli; Li, Xiaona; Luo, Ying; Dai, Haofu

    2011-01-01

    The antioxidant activities of the ethanol, petroleum ether, ethyl acetate, n-butanol and water extract fractions from the seeds of papaya were evaluated in this study. The ethyl acetate fraction showed the strongest DPPH and hydroxyl free radical-scavenging activities, and its activities were stronger than those of ascorbic acid and sodium benzoate, respectively. The n-butanol fraction demonstrated the greatest ABTS? radicals scavenging activity. The ethyl acetate fraction and the n-butanol fraction not only showed higher antioxidant activities than the petroleum ether fraction, water fraction and ethanol fraction, but also showed higher superoxide anion and hydrogen peroxide radicals scavenging activities than those of the other extract fractions. The high amount of total phenolics and total flavonoids in the ethyl acetate and n-butanol fractions contributed to their antioxidant activities. The ethyl acetate fraction was subjected to column chromatography, to yield two phenolic compounds, p-hydroxybenzoic acid and vanillic acid, which possessed significant antioxidant activities. Therefore, the seeds of papaya and these compounds might be used as natural antioxidants. PMID:21788927

  1. Compositional changes during papaya fruit ripening

    International Nuclear Information System (INIS)

    The objective of this study was to investigate compositional changes during ripening of Baladi, Ekostika I and Ekostika II papaya fruit cultivars at 20±1°C and 85% -90% relative humidity. The fruits of the three cultivars exhibited a typical climacteric pattern of respiration with peak of respiration of 82,92 and 98 mg CO2/ kg-hr, reached after 10 days in the three cultivars, respectively. Weight loss, total soluble solids (TSS), total sugars and ascorbic acid content progressively increased during ripening of the three papaya cultivars. More increase in TSS and total sugars was observed after the climacteric peak of respiration. Fruit tissue firmness and total phenolic compounds decreased continuously during ripening in the three cultivars. Reducing sugars, total protein and titratable acidity steadily increased to reach a peak, which coincided with climacteric peak of respiration, and subsequently decreased afterwards. The local Baladi cultivar had a lower respiration rate, more firm and less weight loss during ripening, which may indicate a longer shelf life than the other two introduced cultivars. On the other hand, the introduced cultivars were higher in TSS, total and reducing sugars and ascorbic acid content and lower in titratable acidity and phenolic compounds, which may reflect a better eating quality.(Author)

  2. Nematodos agalladores asociados al cultivo de papaya (carica papaya l.) en el departamento de córdoba, colombia

    OpenAIRE

    Jaraba, Juan de Dios; Lozano, Zaida; Espinosa, Manuel

    2010-01-01

    El objetivo de este trabajo fue identificar los nematodos agalladores asociados al cultivo de papaya (Carica papaya L.) en los municipios de Tierralta, Valencia, Lorica, Montería y Montelíbano que constituyen el área productora de papaya en Córdoba. Se evaluaron variables morfológicas y morfométricas en hembras, machos y juveniles de segundo estadío (J2). En las muestras de suelo se determinó pH, contenido de materia orgánica (MO), conductividad eléctrica (CE) y textura, a fin de corre...

  3. Radiation disinfestation as a quarantine treatment for solo papaya

    International Nuclear Information System (INIS)

    Solo papaya is subject to infestation by the Oriental fruit fly, Bactrocera dorsalis (Hendel). Since ethylene dibromide has been banned for use as a fumigant for fruits, gamma radiation was investigated as an alternative quarantine disinfestation treatment for solo papaya. Results of small-scale laboratory tests showed that 150 Gy completely prevented the emergence of B. dorsalis. For egg-infested solo papaya, pupal recovered decreased with increasing dose while insect survival was not associated with fruit maturity. For larvae-infested ones, insect survival, in general, was highest in the full-ripe stage and lowest in the one-fourth-ripe stage of the fruit. Confirmatory tests of the proposed treatment using more than 100,000 test insects revealed that 150 Gy radiation dose is sufficient to provide Probit 9 quarantine security for solo papaya. (author). 10 refs.; 1 fig.; 4 tabs

  4. Evaluation of fruit leather made from two cultivars of papaya

    Directory of Open Access Journals (Sweden)

    ZUHAIR RADHI ADDAI

    2016-03-01

    Full Text Available Two papaya cultivars were used to manufacture fruit leather. The objective of this study was to formulate papaya leather from locally grown papaya using natural ingredients like pectin, honey and citric acid. The fresh fruits were pureed and mix with natural ingredients, and dried in an oven at 60°C for 12 hours. The physicochemical properties and antioxidant activity were determined.The results showed that fruit leather made from Hongkong cultivar is significantly (P<0.05 higher in sensory parameters as well as physicochemical properties and antioxidant activity. The phenolics content and antioxidant activity increased by process of drying the fruit leather compared to fresh fruits in both papaya cultivars. Therefore, the consumer requirements for healthy and safe food products were respected.

  5. Effects of chemical treatments on fresh-cut papaya.

    Science.gov (United States)

    Albertini, Silvana; Lai Reyes, Andrés Enrique; Trigo, Juliana Moreno; Sarriés, Gabriel Adrián; Spoto, Marta Helena Fillet

    2016-01-01

    Four treatments (control, 0.1% cinnamaldehyde, 0.75% calcium chloride and combination of 0.1% cinnamaldehyde and 0.75% calcium chloride) were used to evaluate chemical effects on shelf life, quality and sensory acceptability of fresh-cut papaya (Carica papaya L.). Papaya slices were packed and covered with polypropylene film, stored at 5 °C; and evaluated after 1, 3, 6, 9, 12, and 15 days for microbiological and physicochemical changes. A sensory evaluation was performed at 1, 3, 6, 9, and 12 days. There was no occurrence of Salmonella, Escherichia coli or psychotropic bacteria. The cinnamaldehyde alone and a combination of cinnamaldehyde and calcium chloride treatments yielded better control of the total coliforms. The combination treatment decreased the CO2 concentration and increased the maintenance of papaya firmness. All the treatments had acceptability. The combination treatment was the most effective treatment for flavor, taste, and preservation until day 12. PMID:26213093

  6. Optical and ultrastructural study of the pollen grain development in hermaphrodite papaya tree (Carica papaya L.)

    OpenAIRE

    Lídia Márcia Silva Santos; Telma Nair Santana Pereira; Margarete de Magalhães Souza; Pedro Correa Damasceno Junior; Fabiane Rabelo da Costa; Beatriz Ferreira Ribeiro; Noil Gomes de Freitas; Messias Gonzaga Pereira

    2008-01-01

    The objective of this study was to describe the pollen grain development in hermaphrodite papaya tree. The flower buds were collected at different stages of the development and the anthers were treated chemically for observation under optical and electronic transmission microscopes. The pollen grain development followed the normal pattern described for the Angiosperms. The pollen grain development was described from meiocyte to the mature pollen grain. In the microsporogenesis, the microspore...

  7. Post-transcriptional gene silencing is involved in resistance of transgenic papayas to Papaya Ringspot Virus

    Czech Academy of Sciences Publication Activity Database

    Ruanjan, P.; Kertbundit, Sunee; Juříček, Miloslav

    2007-01-01

    Roč. 51, č. 3 (2007), s. 517-520. ISSN 0006-3134 Grant ostatní: BIOTEC, NASDA(TH) BT-B-06-PG-14-4503 Institutional research plan: CEZ:AV0Z50380511 Source of funding: V - iné verejné zdroje Keywords : Carica papaya * reverse transcription PCR * COAT PROTEIN GENE Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.259, year: 2007

  8. Influence of thickness on the drying of papaya puree (carica papaya l.) through refractance windowtm technology

    OpenAIRE

    MARA U. OCOR-ZAMORA; Alfredo A Ayala-Aponte

    2014-01-01

    The aim of this study was to evaluate the influence of sample thickness (2, 3 and 4 mm) on the drying kinetics, water activity (aw) and color (DE) of papaya puree slices processed through Refractance WindowTM technology (RWTM). Additionally, the water diffusion coefficient (Deff) was evaluated taking into account the shrinkage effect. The experimental values of moisture were fitted using Newton's and Midillis models. The results showed that the lower the thickness, the faster the drying, the...

  9. ESR detection procedure of irradiated papaya containing high water content

    Science.gov (United States)

    Kikuchi, Masahiro; Shimoyama, Yuhei; Ukai, Mitsuko; Kobayashi, Yasuhiko

    2011-05-01

    ESR signals were recorded from irradiated papaya at liquid nitrogen temperature (77 K), and freeze-dried irradiated papaya at room temperature (295 K). Two side peaks from the flesh at the liquid nitrogen temperature indicated a linear dose response for 3-14 days after the γ-irradiation. The line shapes recorded from the freeze-dried specimens were sharper than those at liquid nitrogen temperature.

  10. ESR detection procedure of irradiated papaya containing high water content

    International Nuclear Information System (INIS)

    ESR signals were recorded from irradiated papaya at liquid nitrogen temperature (77 K), and freeze-dried irradiated papaya at room temperature (295 K). Two side peaks from the flesh at the liquid nitrogen temperature indicated a linear dose response for 3-14 days after the γ-irradiation. The line shapes recorded from the freeze-dried specimens were sharper than those at liquid nitrogen temperature.

  11. ESR detection procedure of irradiated papaya containing high water content

    Energy Technology Data Exchange (ETDEWEB)

    Kikuchi, Masahiro, E-mail: kikuchi.masahiro@jaea.go.j [Quantum Beam Science Directorate, Japan Atomic Energy Agency, 1233 Watanuki-machi, Takasaki, Gunma 370-1292 (Japan); Shimoyama, Yuhei [Quantum Beam Science Directorate, Japan Atomic Energy Agency, 1233 Watanuki-machi, Takasaki, Gunma 370-1292 (Japan); Ukai, Mitsuko [Hokkaido University of Education, 1-2 Hachiman-cho, Hakodate, Hokkaido 040-8567 (Japan); Kobayashi, Yasuhiko [Quantum Beam Science Directorate, Japan Atomic Energy Agency, 1233 Watanuki-machi, Takasaki, Gunma 370-1292 (Japan)

    2011-05-15

    ESR signals were recorded from irradiated papaya at liquid nitrogen temperature (77 K), and freeze-dried irradiated papaya at room temperature (295 K). Two side peaks from the flesh at the liquid nitrogen temperature indicated a linear dose response for 3-14 days after the {gamma}-irradiation. The line shapes recorded from the freeze-dried specimens were sharper than those at liquid nitrogen temperature.

  12. Yellow Krang A New Cultivar Of Papaya For Green Consumption With Tolerance To Papaya Ringspot Virus

    Directory of Open Access Journals (Sweden)

    Janthasri Rapatsa

    2015-12-01

    Full Text Available The development of a papaya cultivar for unripe or green consumption that has resistance to papaya ringspot virus (PRSV began in 2004 by crossing red-fleshed Red Krang cultivar with yellow-fleshed Sai Nampeung cultivar. Numerous diverse hybrids that were obtained were selected in a recurrent selection until 2010. The Yellow Krang line was selected because of the early and high yielding, elongated fruit shape, is yellow and crispy flesh when unripe and suitable for consumption as green papaya, and tolerance to PRSV. From 2010 to 2015 the Yellow Krang line was refined through pure line selection. It was evaluated in four growing areas in Thailand for 3 years. The field trial showed that Yellow Krang can be classified as dwarf, reaching a height of 110-150 cm, early bearing, with mean 105 days to first flower anthesis; an average of 100 flowers and 206 fruits per plant, mean fruit weight 1.2 kg, mean fruit thickness 2.4 cm, mean fruit firmness 7.15 Newtons; high yielding, with mean fresh weight yield of 134.606 kg/hectare; and 24.86% incidence of PRSV.

  13. The Extraction and Properties of Carica papaya Seed Oil

    Directory of Open Access Journals (Sweden)

    Y.M. Li

    2015-04-01

    Full Text Available The main objective of the present study was to evaluate the suitability of Ultrasound-Microwave synergistic Extraction (UMAE for the recovery of papaya seed oil as compared to Ultrasound-Assisted Extraction (UAE. The efficiency of these two methods was assessed by comparing the physicochemical properties and oxidative stability of papaya seed oil. The analytic tests were color, unsaponifiable matters, iodine value, acid value and peroxide value. The fatty acid components were analyzed by GC. Results indicated that the Ultrasound-Microwave synergistic Extraction (UMAE considerably impact on the physicochemical properties of the extracted papaya seed oil, UMAE provided papaya seed oil with obviously lighter color, lower unsaponifiable matters (2.53% and higher oxidative stability (PV, 0.98 m mol/kg than that of UAE. Analysis of fatty acid composition revealed that 13 kinds of components are identified and the total amount of fatty acids accounted for 93.13% of papaya seed oil, the predominant fatty acids in papaya seed oil were oleic (18:1, 72.60%, palmitic (16:0, 18.00%, linoleic acid (18:2, 5.80% and stearic (18:0, 3.60%.

  14. Efficacy of four plant extracts on nematodes associated with papaya in Sindh, Pakistan

    Science.gov (United States)

    This investigation examines the effect of ethanol extracts of four plant species--Azadirachta indica (neem), Withania somnifera (ashwagandha), Tagetes erecta (marigold) and Eucalyptus citriodora (eucalyptus)--against nematodes associated with papaya (Carica papaya), and it assesses their influence o...

  15. Screening of papaya accessions resistant to Papaya lethal yellowing virus and capacity of Tetranychus urticae to transmit the virus

    Directory of Open Access Journals (Sweden)

    Marcos Fernando Basso

    2015-02-01

    Full Text Available The objective of this work was to produce a polyclonal antiserum against the coat protein (CP of Papaya lethal yellowing virus (PLYV and to determine its specificity and sensibility in the diagnosis of the virus, as well as to evaluate the genetic resistance to PLYV in papaya (Carica papaya accessions and to investigate the capacity of the two-spotted spider mite Tetranychus urticae to acquire and transmit PLYV to the plants. Sixty-five papaya accessions were evaluated. For each accession, ten plants were mechanically inoculated using PLYV-infected plant extracts, and three plants were mock inoculated with phosphate buffer alone and used as negative controls. Ninety days after inoculation, newly-emerging systemic leaves were collected from the inoculated plants, and viral infection was diagnosed by indirect Elisa, using polyclonal antiserum sensible to the in vitro-expressed PLYV CP. Viral transmission by T. urticae was evaluated in greenhouse. The experiments were repeated twice. Polyclonal antiserum recognized the recombinant PLYV CP specifically and discriminated PLYV infection from infections caused by other plant viruses. Out of the 65 papaya accessions evaluated, 15 were considered resistant, 18 moderately resistant, and 32 susceptible. The two-spotted spider mite T. urticae was capable of acquiring PLYV, but not of transmitting it to papaya.

  16. THERAPEUTIC POTENTIAL OF CARICA PAPAYA L. LEAF EXTRACT IN TREATMENT OF DENGUE PATIENTS

    OpenAIRE

    CVM Naresh Kumar; V Taranath; A Venkatamuni; Vishnu Vardhan, R.; Y Siva Prasad; U Ravi; DVR Sai Gopal

    2015-01-01

    Carica papaya leaf extracts are known to have many beneficial medicinal properties. Recent studies have claimed possible beneficial effects of Carica papaya leaf extracts in treating patients with dengue infection. The present study aims to evaluate the therapeutic potential of Carica papaya leaf extracts in treating dengue patients. Carica papaya leaf extracts were given to dengue infected patients and their blood samples were daily monitored. Polyserositis condition of the patients was anal...

  17. Origin and domestication of papaya Yh chromosome.

    Science.gov (United States)

    VanBuren, Robert; Zeng, Fanchang; Chen, Cuixia; Zhang, Jisen; Wai, Ching Man; Han, Jennifer; Aryal, Rishi; Gschwend, Andrea R; Wang, Jianping; Na, Jong-Kuk; Huang, Lixian; Zhang, Lingmao; Miao, Wenjing; Gou, Jiqing; Arro, Jie; Guyot, Romain; Moore, Richard C; Wang, Ming-Li; Zee, Francis; Charlesworth, Deborah; Moore, Paul H; Yu, Qingyi; Ming, Ray

    2015-04-01

    Sex in papaya is controlled by a pair of nascent sex chromosomes. Females are XX, and two slightly different Y chromosomes distinguish males (XY) and hermaphrodites (XY(h)). The hermaphrodite-specific region of the Y(h) chromosome (HSY) and its X chromosome counterpart were sequenced and analyzed previously. We now report the sequence of the entire male-specific region of the Y (MSY). We used a BAC-by-BAC approach to sequence the MSY and resequence the Y regions of 24 wild males and the Y(h) regions of 12 cultivated hermaphrodites. The MSY and HSY regions have highly similar gene content and structure, and only 0.4% sequence divergence. The MSY sequences from wild males include three distinct haplotypes, associated with the populations' geographic locations, but gene flow is detected for other genomic regions. The Y(h) sequence is highly similar to one Y haplotype (MSY3) found only in wild dioecious populations from the north Pacific region of Costa Rica. The low MSY3-Y(h) divergence supports the hypothesis that hermaphrodite papaya is a product of human domestication. We estimate that Y(h) arose only ∼ 4000 yr ago, well after crop plant domestication in Mesoamerica >6200 yr ago but coinciding with the rise of the Maya civilization. The Y(h) chromosome has lower nucleotide diversity than the Y, or the genome regions that are not fully sex-linked, consistent with a domestication bottleneck. The identification of the ancestral MSY3 haplotype will expedite investigation of the mutation leading to the domestication of the hermaphrodite Y(h) chromosome. In turn, this mutation should identify the gene that was affected by the carpel-suppressing mutation that was involved in the evolution of males. PMID:25762551

  18. Protection and conservation of Caricaceae germplasm with PRSV resistant transgenic papaya

    Science.gov (United States)

    Papaya ringspot virus (PRSV) is a devastating disease that has a detrimental impact on both commercial papaya production and Caricaceae germplasm conservation. The PRSV coat protein transgenic line 55-1 and derived progeny are resistant to PRSV and have saved the papaya industry in Hawaii. Here we ...

  19. The dsRNA Virus Papaya Meleira Virus and an ssRNA Virus Are Associated with Papaya Sticky Disease.

    Science.gov (United States)

    Sá Antunes, Tathiana Ferreira; Amaral, Raquel J Vionette; Ventura, José Aires; Godinho, Marcio Tadeu; Amaral, Josiane G; Souza, Flávia O; Zerbini, Poliane Alfenas; Zerbini, Francisco Murilo; Fernandes, Patricia Machado Bueno

    2016-01-01

    Papaya sticky disease, or "meleira", is one of the major diseases of papaya in Brazil and Mexico, capable of causing complete crop loss. The causal agent of sticky disease was identified as an isometric virus with a double stranded RNA (dsRNA) genome, named papaya meleira virus (PMeV). In the present study, PMeV dsRNA and a second RNA band of approximately 4.5 kb, both isolated from latex of papaya plants with severe symptoms of sticky disease, were deep-sequenced. The nearly complete sequence obtained for PMeV dsRNA is 8,814 nucleotides long and contains two putative ORFs; the predicted ORF1 and ORF2 display similarity to capsid proteins and RdRp's, respectively, from mycoviruses tentatively classified in the family Totiviridae. The sequence obtained for the second RNA is 4,515 nucleotides long and contains two putative ORFs. The predicted ORFs 1 and 2 display 48% and 73% sequence identity, respectively, with the corresponding proteins of papaya virus Q, an umbravirus recently described infecting papaya in Ecuador. Viral purification in a sucrose gradient allowed separation of particles containing each RNA. Mass spectrometry analysis indicated that both PMeV and the second RNA virus (named papaya meleira virus 2, PMeV2) were encapsidated in particles formed by the protein encoded by PMeV ORF1. The presence of both PMeV and PMeV2 was confirmed in field plants showing typical symptoms of sticky disease. Interestingly, PMeV was detected alone in asymptomatic plants. Together, our results indicate that sticky disease is associated with double infection by PMeV and PMeV2. PMID:27166626

  20. Evaluacin de marcadores genticos para discriminacin entre hembras y hermafroditas de papaya (Carica papaya L.) variedad 'Maradol' / Evaluation of genetic markers for discrimination between females and hermaphrodites of papaya (Carica papaya L.) CV. 'Maradol'

    Scientific Electronic Library Online (English)

    Violeta, Aspeitia-Echegaray; Ma. Alejandra, Torres-Tapia; Dulce V., Mendoza-Rodrguez; M. Humberto, Reyes-Valds.

    2014-09-01

    Full Text Available La papaya (Carica papaya L.) presenta tres tipos sexuales: macho, hembra y hermafrodita, de los cuales solo el ltimo posee valor comercial y calidad de exportacin. Con base en su morfologa, dichos tipos nicamente pueden ser identificados a partir de la floracin. La segregacin del sexo en esta [...] especie se explica con un modelo de un locus multiallico, aunque tiene una base molecular ms compleja ya que puede intervenir ms de un gen. En la papaya 'Maradol' se presentan casi exclusivamente los tipos femenino y hermafrodita, por lo cual en esta variedad se busca solamente la distincin entre ambas formas sexuales. En este trabajo se probaron tres juegos de iniciadores para marcadores SCAR, previamente desarrollados para determinacin del sexo en variedades hawaianas. Los tres marcadores se identifican como T1, T12 y W11. A partir de tejido de 17 plantas identificadas como hembras y 23 hermafroditas de papaya 'Maradol' se encontr que los marcadores T12 y W11 fueron especficos en 100 % para plantas hermafroditas, mientras que no se observ amplificacin para las plantas hembra. el SCAR T1 amplific ADN solamente en algunas plantas hermafroditas. Se concluye que los SCAR T12 y W11 pueden ser utilizados como parte de una tcnica para identificacin temprana del sexo de las plantas de papaya 'Maradol' con fines de plantacin comercial, previa validacin con otras poblaciones de la misma variedad. Abstract in english Papaya (Carica papaya L.) shows three sexual types: male, female and hermaphrodite, from which only the last one has commercial value and export quality. Morphologically, such types are only distinguishable starting at flowering stage. Sex segregation in this species is explained by a multiallelic l [...] ocus, even though it is more complex at the molecular level. In 'Maradol' papaya, the female and hermaphrodite types are almost the only present sexual forms, so that the distinction between these two forms is sought. In this work, three sets of SCAR primers, previously developed for Hawaiian varieties, were tested. The three markers are identified as T1, T12 and W11. From tissue of 17 female and 23 hermaphrodite plants, it was found that the markers T12 and W11 showed 100% specificity for hermaphrodite individuals, with null amplification for female plants. On the other hand, the T1 SCAR primers amplified DNA only in some hermaphrodite plants. It is concluded that T12 and W11 SCAR markers can be used as a part of a technique to identify plant sex in early stages of 'Maradol' papaya for commercial cultivation purpose, after validation with other populations of the same variety.

  1. Effect of ozone on anthracnose physicochemical responses and gene expression in papaya (carica papaya l.)

    OpenAIRE

    Ong, Mei Kying

    2014-01-01

    A study was conducted to investigate the effects of varying levels of ozone (0, 1.5, 2.5, 3.5 or 5.0 ppm) for 96 h on 1. the in vitro and in vivo growth of Colletotrichum gloeosporioides, the causal organism of anthracnose; 2. the reactive oxygen species generation and spore mitochondria of C. gloeosporioides using transmission electron microscope, fluorescence microscope and laser scanning confocal microscope; 3. the production of defence-related enzymes in papaya; 4. microbiological analysi...

  2. OBTAINING ACTIVATED CARBON FROM PAPAYA SEEDS FOR ENERGY STORAGE DEVICES

    Directory of Open Access Journals (Sweden)

    Ariharaputhiran Anitha

    2015-01-01

    Full Text Available Preparation of carbon from papaya (Carica papaya L. seeds activated with ZnCl2 @ 300°C is presented. Characterization of the powder is carried out by elemental analysis, N2 sorption studies, powder x-ray diffractometry, fourier transform infrared spectroscopy, scanning electron microscopy, cyclic voltammetry and galvanostatic charge-discharge studies. Specific capacitance from cyclic voltammetric studies on the carbon is 472 F g-1 in 1M H2SO4 electrolyte when run between -0.1 and 1.0V Vs SCE at 10 mV s-1 scan rate. The proposed carbon sample posses an N2 adsorption isotherm characteristic of mesopore structures. The presence of various surface functional groups like carbonyl and hydroxyl groups coupled with desired physical features of the carbon are presumed to be responsible for fast faradic reactions to offer pseudocapacitive characteristics to the papaya derived carbon. Capacity as well as the cycling stability of the electrode prepared with papaya seed based activated carbon in acid electrolyte seems to be impressive as observed from charge-discharge studies. Thus papaya seed biomass represents a promising new green source of carbon for electrochemical capacitor electrodes and gives a new and valuable dimension to domestic wastes.

  3. Effect of gamma-irradiation on ripening papaya pectin

    International Nuclear Information System (INIS)

    Papaya (Carica papaya, L., var. Sunset) at three initial ripeness stages were irradiated with 0.25, 0.50, 0.75, 1.0, or 1.5 kGy gamma-irradiation and pectin changes during ripening determined. A significant linear relationship was found between irradiation dose and firmness immediately after irradiation. Irradiation had no effect on fruit skin or flesh color of papaya fruit irradiated at the 5 to 30% yellow stage and allowed to ripen. Papaya irradiated when 5 to 30% yellow showed no significant changes in pectin methylesterase activity when ripe. Immediately after irradiation, the pectin in 10 to 30% yellow papaya showed depolymerization and demethoxylation, though no effect on pectin methylesterase activity was detected. There was an increase in water soluble pectin (WSP), while chelator soluble (CSP) and alkali soluble pectin (ASP) decreased, with a significant decline in the methanol content of the ASP fraction. After the 25 to 30% yellow ripeness stage, fruit irradiated at 0.50 to 1.0 kGy had less pectic depolymerization, and had a firmer texture than nonirradiated when ripe. A lower level of WSP and higher levels of CSP and ASP were found in ripe fruit that had been irradiated at 0.5 to 1.0 kGy when 25 to 30% yellow skin with a significant quadratic relationship between irradiation dose and the three pectin fractions. The firmness of these irradiated fruit were retained for two days longer than the nonirradiated control. (author)

  4. INFLUENCIA DE LA OSMOCONGELACIN SOBRE ALGUNAS PROPIEDADES FSICAS DE PAPAYA (Carica papaya L.) / INFLUENCE OF OSMODEHYDROFREEZING ON SOME PHYSICAL PROPERTIES OF PAPAYA (Carica papaya L.)

    Scientific Electronic Library Online (English)

    Alfredo, Ayala Aponte; Martha, Snchez; Hanny, Rodrguez.

    2014-12-01

    Full Text Available La osmo-congelacin (OC) es el proceso combinado de aplicar la deshidratacin osmtica (DO) seguido de la congelacin en un alimento, para mejorar la calidad del producto congelado-descongelado. El objetivo de este trabajo fue evaluar la influencia de la aplicacin de pretratamientos osmticos sobre [...] la prdida de fase lquida (PFL), volumen (V) y color, en trminos de claridad (L*), tono (h) y cambio total de color (?E), en muestras de papaya, almacenadas en congelacin. Las muestras fueron deshidratadas osmticamente hasta 30 y 90 min, alcanzando, respectivamente, contenidos de humedad, de 81,400,69% y 76,240,41%(b.h); para ello, se emple una solucin osmtica (SO) de sacarosa, con 65Brix, a 26,0+0,2C. Las muestras deshidratadas osmticamente fueron congeladas a -40C y, posteriormente, se almacenaron a -18C, durante 10, 20, 30 y 40 das. Como tratamiento control, se utilizaron muestras no tratadas osmticamente (MNT), durante el almacenamiento en congelacin. Los resultados mostraron que, en todos los tratamientos, el tiempo de almacenamiento en congelacin influy significativamente (p Abstract in english The osmo-dehydro-freezing (OC) is the combined process of applying osmotic dehydration (OD) followed by freezing of a food product to improve the quality of frozen-thawed products. The aim of this study was to evaluate the influence of the application of osmotic pretreatments on the loss of liquid p [...] hase (PFL), volume (V) and color in terms of lightness (L*), hue (h), and color change (?E) of papaya frozen stores samples. The osmotically dehydrated samples were treated during 30 and 90 min, reaching moisture content of 81,400,69 and 76,240,41% (wb) respectively. For this, an osmotic solution of sucrose with 65Brix at 26.0C+0.2 was used. The treated samples were frozen at -40C and subsequently stored at -18C for 10, 20, 30 and 40 days. As control osmotically untreated samples (MNT) during frozen storage were used. The results showed that in all treatments the frozen storage time significantly influenced (p

  5. Physico-chemical characteristics of papaya (Carica papaya L.) seed oil of the Hong Kong/Sekaki variety.

    Science.gov (United States)

    Yanty, Noorzianna Abdul Manaf; Marikkar, Jalaldeen Mohammed Nazrim; Nusantoro, Bangun Prajanto; Long, Kamariah; Ghazali, Hasanah Mohd

    2014-01-01

    A study was carried out to determine the physicochemical characteristics of the oil derived from papaya seeds of the Hong Kong/Sekaki variety. Proximate analysis showed that seeds of the Hong Kong/Sekaki variety contained considerable amount of oil (27.0%). The iodine value, saponification value, unsaponifiable matter and free fatty acid contents of freshly extracted papaya seed oil were 76.9 g I2/100g oil, 193.5 mg KOH/g oil, 1.52% and 0.91%, respectively. The oil had a Lovibond color index of 15.2Y + 5.2B. Papaya seed oil contained ten detectable fatty acids, of which 78.33% were unsaturated. Oleic (73.5%) acid was the dominant fatty acids followed by palmitic acid (15.8%). Based on the high performance liquid chromatography (HPLC) analysis, seven species of triacylglycerols (TAGs) were detected. The predominant TAGs of papaya seed oil were OOO (40.4%), POO (29.1%) and SOO (9.9%) where O, P, and S denote oleic, palmitic and stearic acids, respectively. Thermal analysis by differential scanning calorimetry (DSC) showed that papaya seed oil had its major melting and crystallization transitions at 12.4°C and -48.2°C, respectively. Analysis of the sample by Z-nose (electronic nose) instrument showed that the sample had a high level of volatile compounds. PMID:25174674

  6. Simultaneous detection of papaya ringspot virus, papaya leaf distortion mosaic virus, and papaya mosaic virus by multiplex real-time reverse transcription PCR.

    Science.gov (United States)

    Huo, P; Shen, W T; Yan, P; Tuo, D C; Li, X Y; Zhou, P

    2015-12-01

    Both the single infection of papaya ringspot virus (PRSV), papaya leaf distortion mosaic virus (PLDMV) or papaya mosaic virus (PapMV) and double infection of PRSV and PLDMV or PapMV which cause indistinguishable symptoms, threaten the papaya industry in Hainan Island, China. In this study, a multiplex real-time reverse transcription PCR (RT-PCR) was developed to detect simultaneously the three viruses based on their distinctive melting temperatures (Tms): 81.0±0.8°C for PRSV, 84.7±0.6°C for PLDMV, and 88.7±0.4°C for PapMV. The multiplex real-time RT-PCR method was specific and sensitive in detecting the three viruses, with a detection limit of 1.0×10(1), 1.0×10(2), and 1.0×10(2) copies for PRSV, PLDMV, and PapMV, respectively. Indeed, the reaction was 100 times more sensitive than the multiplex RT-PCR for PRSV, and 10 times more sensitive than multiplex RT-PCR for PLDMV. Field application of the multiplex real-time RT-PCR demonstrated that some non-symptomatic samples were positive for PLDMV by multiplex real-time RT-PCR but negative by multiplex RT-PCR, whereas some samples were positive for both PRSV and PLDMV by multiplex real-time RT-PCR assay but only positive for PLDMV by multiplex RT-PCR. Therefore, this multiplex real-time RT-PCR assay provides a more rapid, sensitive and reliable method for simultaneous detection of PRSV, PLDMV, PapMV and their mixed infections in papaya. PMID:26666186

  7. An Analysis on DNA Fingerprints of Thirty Papaya Cultivars (Carica papaya L.), Grown in Thailand with the Use of Amplified Fragment Length Polymorphisms Technique

    OpenAIRE

    Janthasri Ratchadaporn; Katengam Sureeporn; U. Khumcha

    2007-01-01

    The experiment was carried out at the Department of Horticulture, Ubon Ratchathani University, Ubon Ratchathani province, Northeast Thailand during June 2002 to May 2003 aims to identify DNA fingerprints of thirty papaya cultivars with the use of Amplified Fragment Length Polymorphisms (AFLP) technique. Papaya cultivars were collected from six different research centers in Thailand. Papaya plants of each cultivar were grown under field conditions up to four months then leaf numbers 2 and 3 of...

  8. PROCESOS DE CANONIZACIÓN DE LA LITERATURA CHILENA

    Directory of Open Access Journals (Sweden)

    Iván Carrasco M

    2008-11-01

    Full Text Available Este trabajo estudia los procesos de canonización en la literatura chilena y sus relaciones con las identidades socioculturales, desde la generación romántica de 1852 (ó 1842 hasta la última década del siglo XX, delimitando sus autores, textos y metatextos canónicos. La hipótesis que organiza el trabajo es que la literatura chilena se inició en torno a un canon único, que ha sido reemplazado a través del tiempo por cánones plurales, paralelos y contrapuestos.This paper studies the literary canonization processes in Chilean literature andtheir relations with social and cultural identities. Its purpose is to establish the boundaries of the texts and metatexts written by Chilean canonical authors, starting with the romantic generation of 1852 (or 1842 and ending up with the works published in the last decade of 'the XX century. The hypothesis of this essay is that Chilean literature can be said to have its origins focused on a single canon, which is later replaced by a series of plural, parallel and even opposite canons.

  9. Sequence and genome organization of papaya meleira virus infecting papaya in Brazil.

    Science.gov (United States)

    Abreu, Emanuel F M; Daltro, Cleidiane B; Nogueira, Elsa O P L; Andrade, Eduardo C; Aragão, Francisco J L

    2015-12-01

    Papaya sticky disease ('meleira') was first observed in Brazil at the beginning of the 1980s. The disease is characterized by intense latex exudation from the fruit surface that becomes dark as it oxidizes, which makes it difficult to sell. The causal agent, which has been called papaya meleira virus (PMeV), has been identified as an isometric virus particle, approximately 50 nm in diameter, with a double-stranded RNA genome. Here, we report the first complete sequence and organization of the 8.7-kb viral dsRNA genome. Two ORFs coding for a putative coat protein and RNA-dependent RNA polymerase (RdRp) were predicted. In silico analysis revealed that the translated ORF2 contains the conserved domains characteristic of an RdRp protein (pfam02123:RdRP 4), which is a family that includes RdRps from members of the genera Luteovirus, Totivirus and Rotavirus. Evolutionary analysis with amino acid sequences with the RdRps from members of the family Totiviridae and some dsRNA viruses showed that PMeV RdRp did not root itself in any genus. PMID:26370790

  10. INFLUENCE OF THICKNESS ON THE DRYING OF PAPAYA PUREE (Carica papaya L. THROUGH REFRACTANCE WINDOWTM TECHNOLOGY

    Directory of Open Access Journals (Sweden)

    MARA U. OCOR-ZAMORA

    2013-01-01

    Full Text Available El objetivo de este trabajo fue evaluar la influencia del espesor de las muestras (2, 3 y 4 mm sobre las cinticas de secado, actividad de agua (aw y color (?E de rodajas de pur de papaya procesadas por tecnologa de ventana de refractanciaTM (RWTM. Adicionalmente se evalu la difusividad del agua (Deff teniendo en cuenta el encogimiento. Los datos de humedad se ajustaron mediante los modelos de Newton y Midilli. Los resultados mostraron que a menor espesor el secado fue ms rpido, los valores de aw fueron menores y los ?E superiores. Las muestras alcanzaron 0.0652, 0.1132 y 0.2624 g agua/ g slido seco en 60 min para rodajas de 2, 3 y 4 mm, respectivamente. El Modelo de Midilli fue el ms apropiado para predecir las curvas de secado de papaya por RWTM. Deff disminuy a menor espesor y su orden de magnitud fue de 10-10 m2/s.

  11. Empleo de un Recubrimiento Formulado con Propleos para el Manejo Poscosecha de Frutos de Papaya (Carica papaya L. cv. Hawaiana) / Use of a Coating Formulated with Propolis for Postharvest Handling of Papaya (Carica papaya L. cv. Hawaiian) Fruits

    Scientific Electronic Library Online (English)

    Elizabeth, Barrera Bello; Marcela, Gil Loaiza; Carlos Mario, Garca Pajn; Diego Luis, Durango Restrepo; Jess Humberto, Gil Gonzlez.

    2012-06-01

    Full Text Available Resumen. El fruto de papaya es reconocido por las propiedades nutricionales y sensoriales; no obstante, su tiempo de vida til en poscosecha es muy corto. En los ltimos aos, para incrementar la vida de almacenamiento del fruto se ha implementado el uso de recubrimientos como vehculos de agentes a [...] ntimicrobianos y antioxidantes, entre otros. En este trabajo se compar el efecto de dos recubrimientos; cera comercial (control) y cera comercial conteniendo un extracto etanlico de propleos (5% p/v), sobre la vida en poscosecha de frutos de papaya (Carica papaya L. cv. Hawaiana) almacenados a temperatura ambiente (28 2 C) y humedad relativa entre 65 y 70%. El efecto de los recubrimientos se determin mediante el ndice de deterioro de los frutos y el recuento microbiano (mesfilos aerobios, mohos y levaduras). Adicionalmente, se evaluaron durante 12 das las propiedades fisicoqumicas de los frutos (cambio de color, textura, pH, acidez total titulable, prdida de peso y slidos solubles). Los resultados mostraron que las papayas tratadas con el recubrimiento formulado con el extracto de propleos, present un menor deterioro en cuanto a su apariencia y mayor inhibicin del crecimiento de microorganismos durante los primeros 6 das de evaluacin en comparacin con los frutos control; adems, no se observaron diferencias, producto de los recubrimientos, en relacin a las caractersticas fisicoqumicas de los frutos. Abstract in english Abstract. Papaya fruit is known for the nutritional and sensory properties; however, its postharvest shelf life is very short. In recent years, to extend the storage life of this fruit, the use of coatings as a carrier of antimicrobial agents and antioxidants, has been implemented. In this work, the [...] effect of two coatings, commercial wax (control) and commercial wax containing an ethanolic extract of propolis (5% w/v) on the postharvest of papaya (Carica papaya L. cv. Hawaiian) was compared. The fruits were stored at room temperature (28 2 C) and a relative humidity between 65 and 70%. The effect of coatings was determined by the deterioration index of the fruit, and the microbial counts (aerobic mesophiles, molds and yeasts). Additionally, during 12 days were evaluated physicochemical properties of the fruits (color, texture, pH, titratable acidity, weight loss and soluble solids). The results showed that the papaya fruits treated with the coating containing the propolis extract presented less deterioration in their appearance and greater inhibition growth of microorganisms during the first 6 days compared with control fruits; moreover, no differences among the coatings in relation to the physicochemical characteristics of the fruit were observed.

  12. Dengue fever treatment with Carica papaya leaves extracts

    OpenAIRE

    Ahmad, Nisar; Fazal, Hina; Ayaz, Muhammad; Abbasi, Bilal Haider; Mohammad, Ijaz; Fazal, Lubna

    2011-01-01

    The main objective of the current study is to investigate the potential of Carica papaya leaves extracts against Dengue fever in 45 year old patient bitten by carrier mosquitoes. For the treatment of Dengue fever the extract was prepared in water. 25 mL of aqueous extract of C. papaya leaves was administered to patient infected with Dengue fever twice daily i.e. morning and evening for five consecutive days. Before the extract administration the blood samples from patient were analyzed. Plate...

  13. Consumer in-store response to irradiated papayas

    International Nuclear Information System (INIS)

    In this study, purchase behavior of California consumers in response to irradiated papayas is described. The papayas were shipped from Hawaii and irradiated in California under a permit by the Animal and Plant Health Inspection Service and approved by the California Department of Food and Agriculture. Results show that the superior appearance of the irradiated fruit appealed to consumers and that two-thirds or more of the people queried indicated that they would buy irradiated produce. It is noted that this marketing took place in a supportive environment with no protestors present. Informational material was available

  14. A coating of chitosan and propolis extract for the postharvest treatment of papaya (Carica papaya L. cv. Hawaiiana

    Directory of Open Access Journals (Sweden)

    Elizabeth Barrera

    2015-06-01

    Full Text Available Propolis is a natural antimicrobial that can be used asa bioadditive in coatings to control fruit quality losses. The effectof two coatings was evaluated, a control (chitosan, 1% and atreatment (chitosan, 1%; containing propolisethanolic extract, 5%,on the microbiological and physicochemical properties of papayafruits. The chemical profile of the propolis revealed the presenceof fatty acids and their esters, carbohydrates, diterpenic acids,and pentacyclic triterpenes. The fruits covered with the treatmentdemonstrated a reduced deterioration index and infection diameterof the fungus Colletotrichum gloeosporioides, as compared tothe control papayas, postponing the appearance of damage bytwo days. Additionally, the treatment did not significantly affectthe physicochemical properties of the papaya, as compared tothe control. In conclusion, the coating formulated with propolisexhibited an in situ fungicidal and bactericidal effect withoutaltering the physiological changes of the papaya fruit duringstorage.

  15. Effect of maleic hydrazide and waxing on quality and shelf life of papaya (carica papaya L.) fruits

    International Nuclear Information System (INIS)

    The effect of post harvest treatment of maleic hydrazide (MH) with and with out waxing on the quality and shelf-life of Baladi and Ekostika I papaya fruits at 18 ±1°C and 85%-90% relative humidity was evaluated. Maleic hydrazide at 250 and 500 ppm significantly delayed fruit ripening by two and three days in both papaya cultivars, respectively, compared with untreated fruits. The higher the concentration, the more was the delay in fruit ripening. The results also showed that waxing addition to MH resulted in a delay of two more days in fruit ripening that treatment with MH alone. The effect of MH and waxing treatments in delaying papaya fruits ripening was manifested in retarded respiratory climacteric, reduced weight loss and delayed fruit softening and increase in total soluble solids and ascorbic acid content.(Author)

  16. Study of the extraction process of papain from latex of papaya (carica papaya l.) fruits cv. maradol

    OpenAIRE

    Andrade Mahecha, Margarita M; Morales Rodríguez, Olga; Martínez Correa, Hugo A.

    2012-01-01

    In this work, we studied the extraction process of papain, present in the latex of papaya fruit (Carica papaya L.) cv. Maradol.  The variables studied in the extraction of papain were: latex:alcohol ratio (1:2.1 and 1:3) and drying method (vacuum and refractance window).  Papain enzyme responses were obtained in terms of enzymatic activity and yield of the extraction process. The best result in terms of enzyme activity and yield was obtained by vacuum drying and a latex:alcohol ratio of 1:3. ...

  17. Marcadores rapd para la identificación del sexo en papaya (carica papaya l.) en colombia

    OpenAIRE

    Chaves-Bedoya, Giovanni; Pulido, Mauricio; Sánchez-Betancour, Erika; Núñez, Víctor

    2009-01-01

    La determinación del sexo en plantas de papaya es considerado un sistema intrigante, debido a que esta especie presenta tres sexos (macho, hembra y hermafrodita) determinados por un locus multialélico. Además, esta especie no presenta cromosomas sexuales morfológicamente diferenciables. Los marcadores moleculares pueden asociarse a características de interés, como en el presente caso, al sexo en plántulas de papaya. Con el objetivo de identificar marcadores moleculares que permitan una rápida...

  18. Papaya fruit quality management during the postharvest supply chain

    Science.gov (United States)

    Papayas are popular in tropical and subtropical regions and are being exported in large volumes to Europe, the U.S. and Japan. The fruit has excellent taste, exotic flavor and nutritional properties, being rich in vitamins A, C, and antioxidants. However, due to its highly perishable nature it has n...

  19. An ultrasonic system for determining papaya physiological properties

    Science.gov (United States)

    Ibrahim, Sallehuddin; Ramli, Azlin; Yunus, Mohd Amri Md

    2015-05-01

    There is an increasing need for high quality fruit. As such it is important to have a fast, accurate and reliable method for measuring and monitoring the quality of fruit from the field to the consumer. This paper presents an investigation on the use of a non-destructive ultrasonic system which can be used to measure the quality of papaya.

  20. Estimation of papaya leaf area using the central vein length

    Directory of Open Access Journals (Sweden)

    Campostrini Eliemar

    2001-01-01

    Full Text Available Four genotypes of papaya (Carica papaya L. two from the 'Solo' group (Sunrise Solo and Improved Sunrise Solo line 72/12 and two from the 'Formosa' group (Tainung 02 and Known-You 01, grown in Macaé, RJ, Brazil (lat. 22(0 24' S, long. 41(0 42' W, were used in this study. Twenty-five mature leaves from each genotype were sampled four and five months after seedling transplant to the field to determine the length of the leaf central vein (LLCV and the leaf area (LA. According to covariance analyses there were no significant differences in the slope and intercept of the mathematical models calculated for each genotype. Thus, a single mathematical model (Log LA = 0.315 + 1.85 Log LLCV, R²=0.898 was adjusted to estimate the LA using the length of LLCV for the four genotypes. An unique model can be applied to estimate the LA for the four papaya genotypes using LLCV in the range from 0.25 to 0.60 m, and for papaya trees 150 to 180 days after transplanting.

  1. Poltica educativa chilena 1965-2009. Qu oculta esa trama?

    Directory of Open Access Journals (Sweden)

    Mara Anglica Oliva

    2010-01-01

    Full Text Available Se aborda la poltica educativa chilena del perodo 1965-2009, inquirindose, Qu oculta la trama? La conjetura es, la poltica educativa, a excepcin del gobierno de Allende, muestra la institucin del orden neoliberal. Se revisan algunas antinomias, mecanismos de ese orden, que se introduce en1965 con la pedagoga por objetivos y la teora del capital humano. Esta pedagoga, es profundizada en dictadura, junto a la idea de subsidiariedad, que impulsa el cambio del rol del Estado, regulado en la Constitucin de 1980, an vigente, y presente en la Ley General de Educacin. La municipalizacin, la transformacin en el financiamiento y la prdida del estatus de funcionario pblico del profesorado, son un legado de la dictadura. Ello, desperfila el afn democrtico de la actual poltica educativa, transformndola en cmplice de un sistema que genera desigualdad y fragmentacin social.

  2. Nueva metodologa para un ranking de Universidades Chilenas

    Directory of Open Access Journals (Sweden)

    David Rappoport

    2006-12-01

    Full Text Available Este artculo cuestiona la metodologa tradicional de elaboracin de rankings de universidades y sugiere la elaboracin de tablas de posiciones de universidades chilenas construidas sobre la base del ingreso de sus titulados, verificndose su validez estadstica.As, el concepto de ranking se acota al de categora: universidades de primera, segunda y tercera clase. Los resultados arrojan las siguientes conclusiones: primero, existen importantes diferencias en el ingreso entre los titulados de las distintas universidades; segundo, el ranking y la magnitud de los diferenciales medios de ingresos dependen de la carrera considerada, reafirmando la necesidad de elaborar rankings separadamente por carrera, y, tercero, se observa que existe un premio por reputacin entre tradicionalesvs. derivadas, y entre privadas antiguas vs. privadas nuevas (para aquellas ubicadas en la Regin Metropolitana y V Regin.

  3. Callus induction in papaya (Carica papaya L. and synseed production for low temperature storage and cryopreservation

    Directory of Open Access Journals (Sweden)

    Teixeira da Silva Jaime A.

    2014-12-01

    Full Text Available The mid- to long-term preservation of papaya (Carica papaya L. would allow for the safeguarding of important germplasm. In this study, soft friable callus (SFC and hard callus (HC were induced from the first two true leaves of 10-day-old seedlings containing a midrib derived from the germinated seed of two cultivars (‘Rainbow’ and ‘Sunrise Solo’. Following germination on a Murashige and Skoog (MS medium that contained 3% sucrose and was free of plant growth regulators (PGRs, sections of the first true leaves from 10-day-old seedlings were exposed to seven published callus or somatic embryogenesis protocols for zygotic embryos, leaves or hypocotyls. Optimal SFC and HC induction was carried out on a half-strength MS medium following the Fitch (1993 or the Ascêncio-Cabral et al. (2008 protocol, respectively. SFC formed shoots that could then convert to plants when transferred to a full-strength MS medium devoid of PGRs. Plantlets 10-cm tall were acclimatised in two steps: first by in vitro acclimatisation in aerated vessels, the Vitron, under CO2-enriched (3000 ppm CO2, then by the transfer of individually rooted plantlets in Rockwool® blocks to a substrate of soil: pine bark : perlite (1:1:1, v/v/v. SFC and HC were then encapsulated in alginate beads, which were exposed to low temperature storage (LTS at 10°C and 15°C, and also cryopreserved for 30 days. All encapsulated alginate beads that contained SFC, HC or leaf tissue that had been stored under LTS or cryopreserved were able to regenerate callus when placed on an optimal callus induction medium. Plants derived from the control, LTS and cryopreservation protocols, either from SFC or HC, were successfully acclimatised.

  4. DESHIDRATACIÓN OSMÓTICA DE FRUTOS DE PAPAYA HAWAIANA (Carica papaya L. EN CUATRO AGENTES EDULCORANTES OSMOTIC DEHYDRATION OF HAWAIIAN PAPAYA FRUITS (Carica papaya L. USING FOUR SWEETENER AGENTS

    Directory of Open Access Journals (Sweden)

    Margarita Maria Ríos Pérez

    2005-12-01

    Full Text Available Trozos de papaya hawaiiana (Carica papaya L. fueron sometidos a un proceso de osmo-deshidratación usando cuatro agentes edulcorantes: miel de abejas, miel de caña, crema de miel de abejas y sacarosa en medio acuoso a 79 grados Brix, temperatura de 20 ºC y 23 horas de inmersión. Los resultados estadísticos mostraron que el agente de mayor capacidad deshidratante fue la miel de abejas y el menor la sacarosa. Además, los análisis cinéticos indicaron que la máxima transferencia de masa ocurre en las primeras cuatro horas del proceso y la máxima pérdida de masa del producto que puede ser alcanzada fue de 32 % con un contenido de humedad final en los frutos de papaya osmodeshidratada de 41,3 % b.h.Pieces of Hawaiian papaya (Carica papaya L. were subjected to osmotic dehydration using four sweetener agents: honey, molasses, honey cream and sucrose in aqueous solution to 79 degrees Brix, 20 ºC temperature and 23 hours of immersion. The statistical results showed that honey was the sweetener agent with highest osmotic capacity while sucrose had the lowest. The kinetic analysis also showed that the maximum mass transfer occurs during the first four hours of the process and the maximum mass loss of the product that can be attained was 32 % with a final moisture content of 41,3 % w.b.

  5. ALTERNATIVA COMERCIAL PARA EXTENDER VIDA DE ANAQUEL DE PAPAYA 'MARADOL'

    Directory of Open Access Journals (Sweden)

    J. A. Osuna-García

    2009-01-01

    Full Text Available El objetivo de este trabajo fue evaluar el efecto del 1-Metilciclopropeno (1-MCP y etileno (solos o en combinación para manipular el proceso de maduración y extender vida de anaquel de papaya 'Maradol'. Durante el 2007 se realizaron tres ensayos: 1. Efecto del 1-MCP en papaya almacenada en condiciones de mercadeo y refrigeración más mercadeo; 2. Papaya tratada con 1-MCP y etileno y mezcla 1-MCP + etileno; 3. Evaluación comercial del 1-MCP y etileno en papaya. Se analizaron pérdida de peso, color externo, firmeza, color de pulpa y sólidos solubles totales. Se encontró que el 1-MCP a 100 nl·litro-1 retrasó el desarrollo de color externo y pulpa e inhibió el ablandamiento de los frutos. También se observó que el etileno a dosis de 100 ¿l·litro-1 aplicado después del 1-MCP no revirtió el efecto de éste, sin embargo, el 1-MCP aplicado después del etileno redujo la velocidad de ablandamiento y el desarrollo del color en cáscara y pulpa inducido por el etileno. Se concluye que el 1-MCP a 100 nl·litro-1 por 12 horas aplicado después del etileno puede ser una técnica viable para manipular el proceso de maduración y alargar vida de anaquel de papaya 'Maradol'.

  6. Sensitivity of papaya seeds to gamma-irradiation

    International Nuclear Information System (INIS)

    The major problems faced by the papaya industry in Malaysia are the papaya ringspot virus disease and the short shelf-life of the fruit. Efforts have been made to address these problems and they include conventional breeding and selection and biotechnological tools for development of transgenic papayas with PRSV resistance and delayed fruit ripening characteristics. Irradiation-induced changes in papaya for selection of improved varieties could offer another approach to resolve these problems. As the first step, the radio-sensitivity of papaya seed must be established before mass irradiation of the materials to generate the desired variability for evaluation and selection later. For the first approximation dosage, the seeds of two local. varieties, Sekaki and Eksotika were irradiated as either dry seeds or as pre-germinated seeds (soaked overnight in water and surface-dried). The dosage ranged from 0 to 300 Gy. Wet pre-germinated seeds were all killed at 100 Gy while dry seeds did not show loss of viability even at 300 Gy. The dosage for dry seeds was raised to 1000 Gy in the following experiment and the LD 50 (or half-kill) was estimated to be 650 Gy. However, at this dosage, seedling growth was markedly reduced and this dosage may not be suitable for mass irradiation. From the growth data, it was estimated that 525 Gy was the dosage that reduced 50% of growth in height and this dosage was recommended for mass irradiation of dry seeds. For wet, pre-germinated seeds, results from the third experiment indicated that 42.5 Gy was the optimal dosage for mass irradiation. At this dosage, both seed germination and seedling growth was reduced by 50%. (Author)

  7. Antagonism or synergism between papaya ringspot virus and papaya mosaic virus in Carica papaya is determined by their order of infection.

    Science.gov (United States)

    Chávez-Calvillo, Gabriela; Contreras-Paredes, Carlos A; Mora-Macias, Javier; Noa-Carrazana, Juan C; Serrano-Rubio, Angélica A; Dinkova, Tzvetanka D; Carrillo-Tripp, Mauricio; Silva-Rosales, Laura

    2016-02-01

    Antagonism between unrelated plant viruses has not been thoroughly described. Our studies show that two unrelated viruses, papaya ringspot virus (PRSV) and papaya mosaic virus (PapMV) produce different symptomatic outcomes during mixed infection depending on the inoculation order. Synergism occurs in plants infected first with PRSV or in plants infected simultaneously with PRSV and PapMV, and antagonism occurs in plants infected first with PapMV and later inoculated with PRSV. During antagonism, elevated pathogenesis-related (PR-1) gene expression and increased reactive oxygen species production indicated the establishment of a host defense resulting in the reduction in PRSV titers. Polyribosomal fractioning showed that PRSV affects translation of cellular eEF1α, PR-1, β-tubulin, and PapMV RNAs in planta, suggesting that its infection could be related to an imbalance in the translation machinery. Our data suggest that primary PapMV infection activates a defense response against PRSV and establishes a protective relationship with the papaya host. PMID:26765969

  8. In vitro erythrocyte membrane stabilization properties of Carica papaya L. leaf extracts

    OpenAIRE

    Ranasinghe, Priyanga; Ranasinghe, Pathmasiri; W P Kaushalya M. Abeysekera; Premakumara, G. A. Sirimal; Perera, Yashasvi S; Gurugama, Padmalal; Gunatilake, Saman B

    2012-01-01

    Background: Carica papaya L. fruit juice and leaf extracts are known to have many beneficial medical properties. Recent reports have claimed possible beneficial effects of C. papaya L. leaf juice in treating patients with dengue viral infections. This study aims to evaluate the membrane stabilization potential of C. papaya L. leaf extracts using an in vitro hemolytic assay. Materials and Methods: The study was conducted in between June and August 2010. Two milliliters of blood from healthy vo...

  9. In vitro erythrocyte membrane stabilization properties of Carica papaya L. leaf extracts

    OpenAIRE

    Priyanga Ranasinghe; Pathmasiri Ranasinghe; W P Kaushalya M. Abeysekera; G A Sirimal Premakumara; Perera, Yashasvi S; Padmalal Gurugama; Gunatilake, Saman B

    2012-01-01

    Background : Carica papaya L. fruit juice and leaf extracts are known to have many beneficial medical properties. Recent reports have claimed possible beneficial effects of C. papaya L. leaf juice in treating patients with dengue viral infections. This study aims to evaluate the membrane stabilization potential of C. papaya L. leaf extracts using an in vitro hemolytic assay. Materials and Methods: The study was conducted in between June and August 2010. Two milliliters of blood from healthy v...

  10. Evaluation of platelet augmentation activity of Carica papaya leaf aqueous extract in rats

    OpenAIRE

    Swati Patil; Supritha Shetty; Rama Bhide; Shridhar Narayanan

    2013-01-01

    Carica papaya leaves have been used traditionally to treat indigestion, as a vermifuge. Carica papayaleaves have also been shown to possess anti-tumor and immunomodulatory effects. The current study aims at determining the effect of Carica papaya leaves aqueous extract in increasing the platelet count in thrombocytopenic rat model. Aqueous extract of Carica papaya leaves at concentration of 400mg/kg and 800mg/kg were given to cyclophosphamide induced thrombocytopenic rats for a period of fift...

  11. THERAPEUTIC POTENTIAL OF CARICA PAPAYA L. LEAF EXTRACT IN TREATMENT OF DENGUE PATIENTS

    Directory of Open Access Journals (Sweden)

    CVM Naresh Kumar

    2015-07-01

    Full Text Available Carica papaya leaf extracts are known to have many beneficial medicinal properties. Recent studies have claimed possible beneficial effects of Carica papaya leaf extracts in treating patients with dengue infection. The present study aims to evaluate the therapeutic potential of Carica papaya leaf extracts in treating dengue patients. Carica papaya leaf extracts were given to dengue infected patients and their blood samples were daily monitored. Polyserositis condition of the patients was analysed by ultrasonography before and after the completion of Carica papaya leaf extract treatment. Repeated Measures ANOVA and descriptive measures such as mean, standard error values were calculated. Before the administration of Carica papaya leaf extracts the patients had a low WBC and platelet counts. After the administration of Carica papaya leaf extracts there was a good amount of increase in the counts of WBC and platelets (p<0.05 in all the patients and the patients started recovering from dengue infection. Ultrasonographic findings revealed clearance in pleural ascites, pericardiac effusion and peritoneal ascites. Blood analysis reports, ultrasonographic findings, statistical results and patients recovery from dengue infection clearly shows the therapeutic role of Carica papaya leaf extracts in treating dengue infected patients. Further studies are necessary for identification of the compounds present in Carica papaya leaf extract and exploring their therapeutic role in curing dengue infection.

  12. Effect of gamma irradiation in papaya (Carica papaya L.) harvested in three degrees of maturation.; Efeito da irradiacao gama em mamao papaia (Carica papaya L.) colhido em tres pontos de maturacao

    Energy Technology Data Exchange (ETDEWEB)

    Pimentel, Rodrigo Meirelles de Azevedo

    2001-07-01

    The papaya is a fragile tropical fruit with thin skin, susceptible to post harvest diseases and mechanical injuries. Furthermore, it is sensible to low temperatures and at normal conditions it ripens rapidly, increasing the difficulties with storage. The objective of this work was to evaluate the influence of gamma irradiation in papayas harvested in three degrees of maturation, in order to increase shelf life. To accomplish that, papayas were harvested in perfect quality conditions, washed, submitted to carnauba wax and selected by skin coloration into three distinct degrees of maturation: Mat 0, or beginning of yellow coloration; Mat 1, yellow stripes more developed, and Mat 2, one third yellow. Half of them were submitted to irradiation with 0, 75 kGy, while the others became control treatment. They were analyzed in four different periods of conservation, which were 1 day after irradiation (DAI) refrigerated (11 +- 1 deg C), 14 DAI refrigerated, 14 DAI refrigerated + 3 at room temperature (RT = 24 deg +- 2 deg C) and 14 DAI refrigerated + 6 at RT. The effect of irradiation was not influenced by the maturation degree at harvest. Irradiation promoted firmness maintenance of papaya and, therefore, delayed ripening; modified the green color of papaya to a lighter tone, which determined more homogeneity in the skin yellow color development (greater values of L* and b*), and turned the papaya flesh color lighter (rower values of b*). There was no effect of irradiation in papaya weight loss, in the occurrence of diseases, in the development of surface yellow color, in the parameter a* of papaya skin color, in the parameters L*, a* and chroma of flesh color, p H and total soluble solids content. Visual and organoleptic sensorial tests were accomplished with papayas from a new delivery in the conservation period of 14 DAI refrigerated + 3 at RT. In the visual test was evaluated the appearance of papaya in the following treatments: Mat O control, Mat O irradiated, Mat 1 control, Mat 1 irradiated, Mat 2 control e Mat 2 irradiated, in which the first one received lower scores due to the occurrence of ratting on one of the papayas. In the organoleptic test, the parameters firmness, sweetness and acceptance were evaluated for the following treatments: control, Mat O irradiated, Mat 1 irradiated e Mat 2 irradiated. There was no difference between treatments for the sweetness parameter. The irradiated treatments received better scores for firmness as well as papayas irradiated at Mat 1 and 2 for acceptance (author)

  13. Cyanogenesis in glucosinolate-producing plants: Carica papaya and Carica quercifolia

    DEFF Research Database (Denmark)

    Olafsdottir, E.S.; Jørgensen, Lise Bolt; Jaroszewski, Jerzy W.

    Carica papaya, Carica quercifolia, Carica hastata, Caricaceae, Passifloraceae, Biosynthesis, Glucosinolates, Cyanohydrin glycosides, Cyanogenic glycosides, Prunasin, Tetraphyllin B, Cyclopentenylglycine......Carica papaya, Carica quercifolia, Carica hastata, Caricaceae, Passifloraceae, Biosynthesis, Glucosinolates, Cyanohydrin glycosides, Cyanogenic glycosides, Prunasin, Tetraphyllin B, Cyclopentenylglycine...

  14. Situacin actual y perspectivas tecnolgicas para la papaya (Carica papaya L.) en el distrito de Veracruz, Veracruz / Current situation and technological prospects for papaya (Carica papaya L.) in the district of Veracruz, Veracruz

    Scientific Electronic Library Online (English)

    Rebeca, Granados Ramrez; Rafael, Salceda Lopez; Mara del Pilar, Longar Blanco.

    2015-06-01

    Full Text Available La papaya es la tercera fruta tropical ms consumida en el mundo y por su dinamismo comercial es considerada una de las ms importantes desde el punto de vista econmico. Mxico ocupa el sexto lugar como productor mundial con 712 917 t anuales, de las cuales una quinta parte se destina al mercado ex [...] terior. El objetivo de este trabajo fue analizar las innovaciones tecnolgicas utilizadas durante el proceso de produccin de la Carica papaya L. en el DDR 007 Veracruz, e identificar las etapas vulnerables y sugerir estrategias. La metodologa incluy la evaluacin del coeficiente de localizacin, anlisis del proceso de produccin con nfasis en la utilizacin de tecnologas de vanguardia, mediante la obtencin de informacin en campo en las comunidades productoras. Se integr la matriz FODA donde se identificaron las fortalezas y factores crticos, oportunidades y amenazas de la actividad frutcola. Se destacaron las condiciones edafo-climticas para la produccin, elevada rentabilidad y rpido retorno de la inversin (fortalezas). De lo anterior, se puede inferir que asignar mayores superficies de tierra al cultivo de papaya en la zona, con certeza ser una opcin viable para la adquisicin de mayores ingresos econmicos para los productores locales; adems de un creciente consumo y las grandes posibilidades de exportacin (oportunidades). Se subraya la necesidad de adicionar biotecnologa para retrasar el proceso de maduracin, adems obtener versatilidad del fruto para su consumo. Abstract in english Papaya is the third most consumed tropical fruit in the world and its commercial dynamism is considered one of the most important from the economic point of view. Mexico ranks sixth as a world producer with 712 917 t, of which one-fifth goes to foreign markets annually. The aim of this study was to [...] analyse the technological innovations used during production of Carica papaya L. in the DDR 007 Veracruz, and identify vulnerable stages and suggest strategies. The methodology included assessment of the location coefficient, analysis of the production process with emphasis on the use of advanced technologies, by obtaining information field producing communities. A SWOT matrix was integrated where the strengths and critical factors, opportunities and threats identified fruit activity. The leaf-climatic conditions were highlighted for the production of high profitability and rapid return on investment (strengths). From the foregoing is inferred that allocating more land areas with papaya crop in the area, for sure will be an option to acquire higher incomes for local producers; besides increasing consumption and large export possibilities (opportunities). The need to add biotechnology to delay the ripening process is emphasized and also getting versatile fruits for consumption.

  15. La familia Hemiaulaceae (Bacillariophyceae) de las aguas marinas chilenas The family Hemiaulaceae (Bacillariophyceae) from marine Chilean waters

    OpenAIRE

    PATRICIO RIVERA; SERGIO AVARIA; FABIOLA CRUCES

    2003-01-01

    La familia Hemiaulaceae comprende cuatro gneros, sealados previamente para las aguas chilenas con las siguientes especies: Cerataulina pelagica (Cleve) Hendey, Hemiaulus sinensis Greville, H. membranaceus Cleve, Climacodium biconcavum Cleve, Eucampia antarctica (Castracane) Mangin, E. cornuta (Cleve) Grunow y E. zodiacus Ehrenberg. Sin embargo, el anlisis con microscopa fotnica y electrnica de muestras recolectadas en aguas marinas chilenas que contenan representantes de esta familia (...

  16. 77 FR 31294 - Notice of Request for Extension of Approval of an Information Collection; Importation of Papaya...

    Science.gov (United States)

    2012-05-25

    ... Collection; Importation of Papaya From Colombia and Ecuador AGENCY: Animal and Plant Health Inspection... with regulations for the importation of commercial shipments of fresh papaya from Colombia and Ecuador... importation of commercial shipments of fresh papaya from Colombia and Ecuador, contact Ms. Dorothy...

  17. 40 CFR 174.515 - Coat Protein of Papaya Ringspot Virus; exemption from the requirement of a tolerance.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Coat Protein of Papaya Ringspot Virus...-INCORPORATED PROTECTANTS Tolerances and Tolerance Exemptions § 174.515 Coat Protein of Papaya Ringspot Virus; exemption from the requirement of a tolerance. Residues of Coat Protein of Papaya Ringspot Virus are...

  18. EVALUACIÓN DE MARCADORES GENÉTICOS PARA DISCRIMINACIÓN ENTRE HEMBRAS Y HERMAFRODITAS DE PAPAYA (Carica papaya L.) VARIEDAD ‘MARADOL’

    OpenAIRE

    Violeta Aspeitia-Echegaray; Ma. Alejandra Torres-Tapia; Dulce V. Mendoza-Rodríguez; M. Humberto Reyes-Valdés

    2014-01-01

    La papaya ( Carica papaya L.) presenta tres tipos sexuales: macho, hembra y hermafrodita, de los cuales solo el último posee valor comercial y calidad de exportación. Con base en su morfología, dichos tipos únicamente pueden ser identificados a partir de la floración. La segregación del sexo en esta especie se explica con un modelo de un locus multialélico, aunque tiene una base molecular más compleja ya que puede intervenir más de un gen. En la papaya ‘Maradol’ se presentan casi exclusivamen...

  19. Caractersticas fenotpicas y agronmicas de seis genotipos de papaya (Carica papaya L. de Tuxpan, Guerrero, Mxico

    Directory of Open Access Journals (Sweden)

    Jos ngel Alcntara Jimnez

    2010-07-01

    Full Text Available En Mxico la produccin de papayo (Carica papaya L. es afectada por factores genticos, ambientales y biticos. Destacan los daos provocados por el virus de la mancha anular, el cual reduce considerablemente la cantidad y calidad de la fruta; por eso es muy importante, conocer la productividad de los genotipos en un ambiente determinado. La presente investigacin se realiz con el objetivo de evaluar el desarrollo y las caractersticas agronmicas de seis genotipos de papayo. El estudio se llev a cabo en la localidad de Tuxpan, Guerrero, donde se cultivaron los genotipos: R4M3, Maradol, Criolla, Red Lady, Zapote y R5M2. Como diseo experimental se utiliz el de bloques completos al azar, con cuatro repeticiones. Las variables evaluadas fueron: altura al primer fruto, dimetro del cuello, altura de la planta, nmero de das a la cosecha, rendimiento de frutos (t/ha, nmero, dimetro y longitud de frutos, concentracin de azcares (grados Brix, textura, color de la pulpa y epicarpio de frutos maduros. Los datos de las variables se sometieron a anlisis de varianza y prueba de comparacin de medias de Tukey. En relacin a la adaptacin de los genotipos: todos los genotipos se desarrollaron y produjeron frutos en las condiciones de trpico seco. La variedad Maradol fue la ms sobresaliente, presentando un porte menor, mayor precocidad y productividad, textura dura de pulpa, alta concentracin de azcares, buen tamao y color.

  20. Ripening behavior of papaya (Carica papaya L.) exposed to gamma irradiation

    International Nuclear Information System (INIS)

    Papaya fruit treated with 250 Gy of γ-irradiation frequently softened more uniformly than non-irradiated fruit. Fruit with less than 25% of their surface colored yellow placed immediately into storage at 10 °C after irradiation developed skin scald. This was prevented by delaying storage by 12 h. Fruit that were irradiated when 30% of the skin was yellowed softened at a slower rate than non-irradiated fruit. There was no difference in softening rate between irradiated and non-irradiated fruit at the mature green stage. Fruit stored for 14 days at 10 °C before returning to 25 °C had a slightly slower rate of softening than fruit allowed to ripen at 25 °C without storage. Premature flesh softening occurred occasionally in fruit that had between 8 and 18% of the skin yellow and 70–90% flesh coloring when irradiated. Premature softening occurred in the tests run on fruit that were harvested during the warmer months; fruit harvested during the cooler months did not show the condition. The conditions of growth that predispose fruit to more rapid softening following γ-irradiation were not determined

  1. COMUNEROS ANDINOS EN LA ADMINISTRACIÓN CHILENA (ARICA, 1880-1929

    Directory of Open Access Journals (Sweden)

    ALBERTO DÍAZ ARAYA

    2011-05-01

    Full Text Available Resumen Se evalúa el impacto generado en los sectores rurales andinos de Arica por la implementación de la estructura y burocracia administrativa chilena post Guerra del Pacífico. A través de registros documentales se hace explícita la participación de comuneros indígenas en la trama estatal chilena en un período de conflicto político y de identidades nacionales. Palabras claves: Comunidad andina – administración chilena – Arica y Tacna. Abstract Assesses the impact generated in the rural Andes Arica for implementing the administrative structure and bureaucracy in Chile after the Pacific War. Through documentary record makes explicit the participation of indigenous community members in the plot Chilean state over a period of political and national identities. Keywords : Andean Community - Chilean government - Arica and Tacna.

  2. Dengue fever treatment with Carica papaya leaves extracts.

    Science.gov (United States)

    Ahmad, Nisar; Fazal, Hina; Ayaz, Muhammad; Abbasi, Bilal Haider; Mohammad, Ijaz; Fazal, Lubna

    2011-08-01

    The main objective of the current study is to investigate the potential of Carica papaya leaves extracts against Dengue fever in 45 year old patient bitten by carrier mosquitoes. For the treatment of Dengue fever the extract was prepared in water. 25 mL of aqueous extract of C. papaya leaves was administered to patient infected with Dengue fever twice daily i.e. morning and evening for five consecutive days. Before the extract administration the blood samples from patient were analyzed. Platelets count (PLT), White Blood Cells (WBC) and Neutrophils (NEUT) decreased from 176×10(3)/µL, 8.10×10(3)/µL, 84.0% to 55×10(3)/µL, 3.7×10(3)/µL and 46.0%. Subsequently, the blood samples were rechecked after the administration of leaves extract. It was observed that the PLT count increased from 55×10(3)/µL to 168×10(3)/µL, WBC from 3.7×10(3)/µL to 7.7×10(3)/µL and NEUT from 46.0% to 78.3%. From the patient feelings and blood reports it showed that Carica papaya leaves aqueous extract exhibited potential activity against Dengue fever. Furthermore, the different parts of this valuable specie can be further used as a strong natural candidate against viral diseases. PMID:23569787

  3. Acarofauna associated to papaya orchards in Veracruz, Mexico

    Directory of Open Access Journals (Sweden)

    Marycruz Abato-Zárate

    2014-01-01

    Full Text Available Mexican agriculturists have recently noticed strong increases of mite infestations in papaya (Carica papaya L. 1753 orchards. A list of mite species associated with papaya leaves was constructed to determine the species responsible for high infestations and to identify predaceous mites as potential biological control agents. Mites were collected from three foliage strata (high, middle and low, in seven municipalities of central Veracruz State. Leaves were processed by washing and sieving. Identified species included four tetranychids: Eotetranychus lewisi (McGregor 1943, Eutetranychus banksi (McGregor 1914, Tetranychus merganser Boudreaux 1954, and Tetranychus urticae Koch 1836; as well as three phytoseiids: Euseius hibisci (Chant 1959, Galendromus helveolus (Chant 1959 and Phytoseiulus macropilis (Banks 1905, with the first two species being the most abundant. The vagrant eriophyid Calacarus citrifolii Keifer 1955 was collected in three municipalities, in the low stratum. Neither the broad mite, Polyphagotarsonemus latus (Banks 1904, nor the carmine spider mite, Tetranychus cinnabarinus (Boisduval 1867, were collected, although these species were previously recorded from this area. None of the Phytoseiid species found can be considered a recently established species; their potential as biological control agents is discussed.

  4. A rapid detection for irradiated fresh papayas using ESR spectroscopy

    International Nuclear Information System (INIS)

    The detections of radicals induced in irradiated both fresh and dried papayas were carried out at liquid nitrogen and room temperature using electron spin resonance (ESR) spectroscopy. Fresh papayas irradiated by the γ-rays were separated into flesh and skin that subjected to the ESR detection. The ESR spectra of the specimen at liquid-nitrogen temperature were observed clearly at a week after the γ-irradiation. Those signals were consisted from main peak at g=2.000 and side peaks at g=2.018 and 1.982. They showed a linear response against the dose. Furthermore, the side peaks from freeze-dried papaya flesh stored at 4degC were observed clearly at two weeks after the γ-irradiation. Those signals also showed the linear dose-response. The detection scheme for irradiated fresh fruits can be done by two stages: 1) A screening test of ESR signals at liquid nitrogen temperature using fresh samples. 2) A room temperature ESR measurement using freeze-dry samples. (author)

  5. Interacciones tempranas y gnero infantil en familias monoparentales chilenas

    Directory of Open Access Journals (Sweden)

    Marcia Olhaberry Huber

    2012-01-01

    Full Text Available Se realiz un estudio transversal de las interacciones madre-hijo(a en familias monoparentales chilenas de bajo ingresos, considerando las diferencias segn el gnero infantil. Participan 80 dadas, con 39 nias y 41 nios entre los 4 y 15 meses y se evala la calidad de las interacciones con el instrumento CARE-Index. Se observan altos puntajes en depresin y estrs materno, as como un promedio en la calidad de la interaccin que indica necesidad de intervencin, mostrando lo anterior vulnerabilidad en las dadas del estudio. Las dadas con infantes de gnero masculino muestran interacciones significativamente ms difciles con sus madres que las de gnero femenino, quienes a su vez muestran interacciones significativamente ms pasivas que los infantes de gnero masculino. Los tamaos de efecto observados en las diferencias son medianos en ambos casos. Se observa tambin complementariedad en las conductas de madres e hijos(as, obtenindose correlaciones positivas y significativas entre sensibilidad materna y cooperatividad infantil, control materno y las conductas difciles de los infantes, as como entre la conducta no responsiva de las madres y la actitud pasiva en los nios(as. Se discuten los hallazgos a partir de las teoras existentes en relacin a gnero infantil e interacciones tempranas, en el contexto de familias monoparentales a cargo de la madre.

  6. Promover la salud mental con mujeres chilenas en desventaja social

    Directory of Open Access Journals (Sweden)

    M Soledad Rivera

    2004-01-01

    Full Text Available Se descrive una parte de la experiencia del proyecto Chileno/Canadiense: “Promover la salud mental de las mujeres chilenas en desventaja social: Los Secretos de Maruja”. Se describen los objetivos, las etapas del proceso, de intervención y los resultados de las evaluaciones preliminares. Con la participación de las mujeres usuárias de los centros de salud, profesionales y autoridades de la comuna de La Pintana, se construyó, aplicó y evaluó un proyecto de intervención para apoyar a las mujeres en la toma de decisiones que favorezcan su salud mental personal, familiar y social. La intervención contempló el diseño, producción, distribución y evaluación de 12 folletos educativos tipo calendarios, cada uno respecto a tres temas de salud mental identificados por las mismas mujeres: Identidad y autoestima, Vínculo y Comunicación y Autocuidado. Además se involucró a las enfermeras que trabajan en los Centros de Salud, quienes incorporaron esta estrategia a la atención de salud habitual y evaluaron su impacto en la identidad, autonomía profesional y en la mejoría de la calidad del cuidado

  7. Effect of heat treatment on ethylene and CO2 emissions rates during papaya (Carica papaya L.) fruit ripening

    Science.gov (United States)

    da Silva, M. G.; Santos, E. O.; Sthel, M. S.; Cardoso, S. L.; Cavalli, A.; Monteiro, A. R.; de Oliveira, J. G.; Pereira, M. G.; Vargas, H.

    2003-01-01

    Ripening studies of nontreated and treated papaya (papaya L) are accomplished by monitoring the ethylene and CO2 emission rates of that climacteric fruit, to evaluate its shelf life. The treatments simulate the commercial Phitosanitarian process used to avoid the fly infestation. Ethylene emission was measured using a commercial CO2 laser driven photoacoustic setup and CO2, using a commercial gas analysis also based on the photothermal effect. The results show a marked change in ethylene and CO2 emission rate pattern for treated fruits when compared to the ones obtained for nontreated fruits and a displacement of the climacteric pick shown that the treatment causes a decrease of shelf life of fruit.

  8. Efecto de la madurez, geometría y presión sobre la cinética de transferencia de masa en la deshidratación osmótica de papaya (Carica papaya L., var. Maradol)

    OpenAIRE

    Lina Marcela Chavarro-Castrillón; Claudia Isabel Ochoa-Martínez; Alfredo Ayala-Aponte

    2006-01-01

    Las papayas obtenidas en el raleo (papayas menos desarrolladas entresacadas de los árboles para beneficiar el crecimiento de las otras) generalmente se descartan. Alternativamente, estas papayas pueden secarse por un proceso de deshidratación osmótica y secado térmico convencional para usarlas como snacks o como ingrediente para otros productos. Se comparó la cinética de transferencia de masa en la deshidratación osmótica de papayas de raleo frente a papayas desarrolladas con diferente grado ...

  9. Papaya is not a host for Tomato Yellow Leaf Curl Virus

    Science.gov (United States)

    The economic value of tomato production is threatened by tomato yellow leaf-curl virus TYLCV and its vector, the silverleaf whitefly Bemisia tabaci biotype B (Gennadius) (Hemiptera: Aleyrodidae). Use of papaya Carica papaya L. as a banker plant for a whitefly parasitoid shows promise as a whitefly m...

  10. A Current Overview of the Papaya meleira virus, an Unusual Plant Virus

    Directory of Open Access Journals (Sweden)

    Paolla M. V. Abreu

    2015-04-01

    Full Text Available Papaya meleira virus (PMeV is the causal agent of papaya sticky disease, which is characterized by a spontaneous exudation of fluid and aqueous latex from the papaya fruit and leaves. The latex oxidizes after atmospheric exposure, resulting in a sticky feature on the fruit from which the name of the disease originates. PMeV is an isometric virus particle with a double-stranded RNA (dsRNA genome of approximately 12 Kb. Unusual for a plant virus, PMeV particles are localized on and linked to the polymers present in the latex. The ability of the PMeV to inhabit such a hostile environment demonstrates an intriguing interaction of the virus with the papaya. A hypersensitivity response is triggered against PMeV infection, and there is a reduction in the proteolytic activity of papaya latex during sticky disease. In papaya leaf tissues, stress responsive proteins, mostly calreticulin and proteasome-related proteins, are up regulated and proteins related to metabolism are down-regulated. Additionally, PMeV modifies the transcription of several miRNAs involved in the modulation of genes related to the ubiquitin-proteasome system. Until now, no PMeV resistant papaya genotype has been identified and roguing is the only viral control strategy available. However, a single inoculation of papaya plants with PMeV dsRNA delayed the progress of viral infection.

  11. Effect of papaya trunk angle on infestation by white peach scale, Pseudaulacaspis pentagona (Hemiptera: Diaspididae)

    Science.gov (United States)

    Two papaya (Carica papaya L.) seedlings growing in one planting hole often results in angular or non-vertical growth of the trees. Data on trunk angularity, or leaning, (deviation from the vertical line of reference) and white peach scale, Pseudaulacaspis pentagona Targioni-Tozzetti (Hemiptera: Dias...

  12. Evaluating Hawaii-Grown Papaya for Resistance to Internal Yellowing Disease Caused by Enterobacter cloacae

    Science.gov (United States)

    Papaya (Carica papaya L.) cultivars and breeding lines were evaluated for resistance to Enterobacter cloacae (Jordan) Hormaeche & Edwards, the bacterial causal agent of internal yellowing disease (IY), using a range of concentrations of the bacterium. Linear regression analysis was performed and IY ...

  13. The Papaya Y Chromosome Evolved Recently and Shows Gene Paucity and DNA Sequence Expansion

    Science.gov (United States)

    Sex chromosomes in flowering plants, in contrast to those in animals, evolved relatively recently and only a few are heteromorphic. At cytological level, the sex chromosomes of papaya appear homomorphic, nevertheless, we are finding the papaya Y chromosome shows features of incipient sex chromosome ...

  14. Hypoglycemic effect of Carica papaya leaves in streptozotocin-induced diabetic rats

    Directory of Open Access Journals (Sweden)

    Juárez-Rojop Isela Esther

    2012-11-01

    Full Text Available Abstract Background Traditional plant treatment for diabetes has shown a surging interest in the last few decades. Therefore, the purpose of this study was to assess the hypoglycemic effect of the aqueous extract of C. papaya leaves in diabetic rats. Several studies have reported that some parts of the C. papaya plant exert hypoglycemic effects in both animals and humans. Methods Diabetes was induced in rats by intraperitoneal administration of 60 mg/kg of streptozotocin (STZ. The aqueous extract of C. papaya was administered in three different doses (0.75, 1.5 and 3 g/100 mL as drinking water to both diabetic and non-diabetic animals during 4 weeks. Results The aqueous extract of Carica papaya (0.75 g and 1.5 g/100 mL significantly decreased blood glucose levels (pC. papaya could help islet regeneration manifested as preservation of cell size. In the liver of diabetic treated rats, C. papaya prevented hepatocyte disruption, as well as accumulation of glycogen and lipids. Finally, an antioxidant effect of C. papaya extract was also detected in diabetic rats. Conclusions This study showed that the aqueous extract of C. papaya exerted a hypoglycemic and antioxidant effect; it also improved the lipid profile in diabetic rats. In addition, the leaf extract positively affected integrity and function of both liver and pancreas.

  15. Cloning Of Organ-Specific Genes from Papaya Using cDNA-AFLP

    Science.gov (United States)

    Papaya is a fast growing and productive fruit crop in tropical and subtropical regions worldwide. Papaya industry has benefited from application of molecular biology and biotechnology for crop improvement, particularly for viral disease resistance using transgenic varieties. The goal of this researc...

  16. Atypical internal yellowing of papaya fruit in Hawaii caused by Enterobacter sakazakii

    Science.gov (United States)

    Internal yellowing (IY), characterized by yellow discolored tissue around the papaya (Carica papaya) seed cavity, diffuse margins and the presence of a distinctly rotten odor, was first reported in 1987. These symptoms were associated with the causal agent Enterobacter cloacae. Here we report the fo...

  17. An umbra-like virus of papaya discovered in Ecuador: detection, occurrence and phylogenetic relatedness

    Science.gov (United States)

    Double-stranded RNA (dsRNA) extractions from papaya leaves infected with Papaya ringspot virus (PRSV) revealed the presence of an unusual 4kb band, in addition to the presumed PRSV-associated 10kb band. Partial sequence of RT-PCR products from the 4kb dsRNA revealed homology to genomes of several me...

  18. Molecular characterization and pathogenicity of Erwinia spp. associated with pineapple [Ananas comosus (L. Merr.] and papaya (Carica papaya L.

    Directory of Open Access Journals (Sweden)

    Ramachandran Kogeethavani

    2015-12-01

    Full Text Available The Erwinia species are well-known pathogens of economic importance in Malaysia causing serious damage to high-value fruit crops that include pineapple [Ananas comosus (L. Merr.] and papaya (Carica papaya L..The 16S rRNA sequence using eubacteria fD1 and rP2 primers, identified two bacteria species; Dickeya zeae from pineapple heart rot, and Erwinia mallotivora from papaya dieback. Phylogenetic analysis based on the neighbor-joining method indicated that all the bacterial isolates clustered in their own taxa and formed monophyletic clades. From the pathogenicity test, all isolates of D. zeae and E. mallotivora showed pathogenic reactions on their respective host plants. Genetic variability of these isolates was assessed using repetitive sequence-based PCR (rep-PCR fingerprinting. The results indicated interspecies, and intraspecies variation in both species’ isolates. There were more polymorphic bands shown by rep-PCR fingerprints than enterobacterial repetitive intergenic consensus (ERIC and BOX- PCRs, however both species’ isolates produced distinguishable banding patterns. Unweighted pair-group method with arithmetic averages (UPGMA cluster analysis indicated that all Dickeya and Erwinia isolates from the same species were grouped in the same main cluster. Similarity among the isolates ranged from 77 to 99%. Sequencing of 16S rRNA using eubacteria fD1 and rP2 primers, and rep-PCR fingerprinting revealed diversity among Dickeya and Erwinia isolates. But this method appears to be reliable for discriminating isolates from pineapple heart rot and papaya dieback.

  19. EFEITOS DO CAPITAL INTELECTUAL SOBRE O DESEMPENHO FINANCEIRO EM EMPRESAS BRASILEIRAS E CHILENAS

    Directory of Open Access Journals (Sweden)

    Salete Turra

    2015-06-01

    Full Text Available O objetivo da pesquisa foi verificar a influncia do Capital Intelectual medido pelo Valor Agregado do Capital Intelectual (VACI sobre o desempenho financeiro em empresas brasileiras e chilenas no ano de 2013. Realizou-se uma pesquisa descritiva, documental, com abordagem quantitativa por meio da tcnica estatstica correlao cannica. Os dados foram coletados na base de dados da Tomnson. Os resultados do estudo demonstraram correlao cannica existente entre as variveis que compem o VACI com as variveis de desempenho financeiro tanto no Brasil como no Chile. Desse modo, concluiu-se que o capital intelectual influencia significativamente o desempenho financeiro das empresas brasileiras e chilenas.

  20. Confiabilidad y validez de la versión chilena de

    OpenAIRE

    León Mayer, Elizabeth; Asún Salazar, Domingo; Folino, Jorge Óscar

    2010-01-01

    Antecedentes. En Chile, al igual que en otros países latinoamericanos, diversas modificaciones judiciales y el interés de los profesionales en sistematizar sus métodos de evaluación en psicopatía promovieron la traducción, adaptación y revisión de las propiedades psicométricas de la Hare Psychopathy Checklist Revised (PCL-R). Objetivo. Evaluar aspectos de la confiabilidad y validez de la versión chilena del PCL-R en población forense masculina chilena. Material y métodos. Se realizó tr...

  1. Hurdle technology to preserve the 'Golden' papaya post harvest quality

    International Nuclear Information System (INIS)

    With the purpose of evaluating a combination of post harvest techniques on papaya Golden storage, the following treatments were investigated: carton boxes packaging (CP); CP + plastic bag of PEBD - low density polyethylene film, with 0.05mm of thickness (PE); CP + PEBD with 0.025mm of thickness impregnated with mineral ethylene scavenger (PEAbs); gamma-irradiation (0.4 kGy and 0.7 kGy); and refrigerated storage at 10 deg C e 90% of relative humidity for 2, 5, 10, 15, 20, 25, 30 and 35 days plus five days under environmental conditions to allow ripening and to simulate the product marketing. To evaluate the effects on fruit quality the following measurements were taken: - first experiment, disease incidence, skin color and loss of turgidity (as visual variables); skin and flesh color, weight loss, flesh firmness, total soluble solids, total titratable acidity, pH, lycopene and beta-carotene composition; - second experiment, sensorial analysis on samples submitted to the treatments with better results from the first experiment. Post harvest diseases were the main factor responsible for discarding fruits. Percentage of weight loss was higher on papayas stored without plastic packaging. Time affected the flesh firmness (F = 95%). However, there wasn't influence of the treatments on that parameter. A significant effect on skin color was observed with interaction between irradiation (0.4 and 0.7 kGy) and storage period. Once reached the edible stage, irradiated papayas presented more uniformity on skin color. For beta-carotene and Lycopene, the mean values were 1.27 to 1.79 and 19.16 to 23.90 mug/ml of flesh, respectively. But those substances weren't affected by the combined methods or the time. The same behavior were observed for total soluble solids, total titratable acidity and pH (mean values of 11.53 to 12.20 deg Brix, 0.117 to 0.136 g of citric acid/100g of flesh and 4.91 to 5.04, respectively). On sensory evaluation, the judges didn't get to detect significant differences among papayas submitted to different treatments. A synergism was verified on the techniques combination, with the best results obtained from the association of CP + PEabs + gamma-irradiation at 0.4 kGy, which reached a total storage period of 35 days. Thus, that is the post harvest hurdle technology recommend for exporting 'Golden' papayas to markets with quarantine restrictions to fruit-flies (author)

  2. Heat shock and gamma radiation in the quarantine process of Papaya CV. Solo(Carica papaya L.)

    International Nuclear Information System (INIS)

    Full text: To export, it is not only necessary to achieve quality standards, but also, quarantine standards. The main function of irradiation is quarantine control of fruit flies, therefore it is necessary to combine with treatments which focus at keeping fruit quality. The objective of this work was the evaluation of the quality of papaya submitted to heat treatment followed by irradiation at quarantine doses. Papayas were selected green and separated in treatments: a) control; irradiation at doses; b) 250, c) 500 Gy; d) hot water at 60 deg C for 30 seconds; and combined to doses e) 250, f) 500 Gy. The papayas were stored at 21 (± 1 deg C) with relative humidity of 85 - 90%, and then after 8 days, they were evaluated by their soluble solid content, pH, titratable acidity, firmness, decay index and internal color. The analysis of mass loss and external color were executed at 0, 2, 4 and 7 days after irradiation. It was observed that in fruits submitted to hot water (d, e, f) there were less decay and in combined treatments (e, f) it was verified higher firmness. In the second day, irradiated treatments (b, c, e, f) were more yellow than the others. As to the other parameters evaluated, there were no differences between treatments (author)

  3. Heat shock and gamma radiation in the quarantine process of Papaya CV. Solo(Carica papaya L.)

    Energy Technology Data Exchange (ETDEWEB)

    Pimentel, Rodrigo M.A.; Walder, Julio M.M. [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil)]. E-mails: rmapiment@cena.usp.br; jmwalder@cena.usp.br; Marcondes, Yvens E.M. [Universidade Metodista de Piracicaba (UNIMEP), SP (Brazil)]. E-mail: yvensmar@unimep.br

    2007-07-01

    Full text: To export, it is not only necessary to achieve quality standards, but also, quarantine standards. The main function of irradiation is quarantine control of fruit flies, therefore it is necessary to combine with treatments which focus at keeping fruit quality. The objective of this work was the evaluation of the quality of papaya submitted to heat treatment followed by irradiation at quarantine doses. Papayas were selected green and separated in treatments: a) control; irradiation at doses; b) 250, c) 500 Gy; d) hot water at 60 deg C for 30 seconds; and combined to doses e) 250, f) 500 Gy. The papayas were stored at 21 ({+-} 1 deg C) with relative humidity of 85 - 90%, and then after 8 days, they were evaluated by their soluble solid content, pH, titratable acidity, firmness, decay index and internal color. The analysis of mass loss and external color were executed at 0, 2, 4 and 7 days after irradiation. It was observed that in fruits submitted to hot water (d, e, f) there were less decay and in combined treatments (e, f) it was verified higher firmness. In the second day, irradiated treatments (b, c, e, f) were more yellow than the others. As to the other parameters evaluated, there were no differences between treatments (author)

  4. Evaluation of platelet augmentation activity of Carica papaya leaf aqueous extract in rats

    Directory of Open Access Journals (Sweden)

    Swati Patil

    2013-01-01

    Full Text Available Carica papaya leaves have been used traditionally to treat indigestion, as a vermifuge. Carica papayaleaves have also been shown to possess anti-tumor and immunomodulatory effects. The current study aims at determining the effect of Carica papaya leaves aqueous extract in increasing the platelet count in thrombocytopenic rat model. Aqueous extract of Carica papaya leaves at concentration of 400mg/kg and 800mg/kg were given to cyclophosphamide induced thrombocytopenic rats for a period of fifteen days. Blood was withdrawn at various time intervals to determine the platelet count. Also, the clotting time was determined on the 15th day of the study by capillary method. Carica papaya leaf extract was found to increase the platelet count and also to decrease the clotting time in rats. The study aims at determining the possible effects of papaya leaves in thrombocytopenia occurring in dengue fever.

  5. Histopathological changes in Wistar albino rats exposed to aqueous extract of unripe Carica papaya

    Directory of Open Access Journals (Sweden)

    Taofeeq Oduola

    2010-05-01

    Full Text Available Background: Exposure of animals to xenobiotics may or may not trigger adverse response at cellular levels. Aqueous extract of unripe Carica papaya is consumed by sickle cell patients as antisickling agent in Western Nigeria. Aim: This study was undertaken to investigate the effects of Carica papaya on certain organs in Wister albino rats exposed to aqueous extract of unripe Carica papaya. Materials and Methods: Different doses of aqueous extract of unripe Carica papaya were administered orally daily for 42 days to six groups of rats. At the end of exposure, the animals were sacrificed and tissue sections were prepared from livers, kidneys, hearts and small intestines using standard techniques. Results: Histopathological results showed that no pathological changes were observed in tissue sections of experimental animals when compared with tissue sections of the same organs in control animals. Conclusion: No pathological changes were elicited in the organs of rats exposed to aqueous extract of unripe Carica papaya.

  6. Evaluation of Antimicrobial Properties of Edible Surface Coating Based on Carrageenan Conjugated with Silver Nanoparticles on Sekaki Papaya (Carica Papaya cv. Sekaki): A New Antimicrobial Edible Coating

    International Nuclear Information System (INIS)

    Antibacterial properties of edible surface coating based on carrageenan incorporated with silver nanoparticles (SNPs), was investigated against Escherichia coli and Staphylococcus aureus to obtain optimum concentration of SNPs. Results obtained indicate that SNPs with concentration of 40 ml L-1 effectively inhibited the growth of both Gram negative and Gram positive bacteria. Sekaki papaya fruits were then coated with prepared edible coating formulation comprising of carrageenan (0.8 % w/v) and glycerol (1.0 % w/v) with and without SNPs (40 ppm) and stored at ambient conditions (26±2 degree Celsius and 60±10 % RH). Microbial analysis of coated and uncoated papaya samples during storage indicated that the edible coating comprising of carrageenan, glycerol and SNPs, strongly inhibited the growth of fungus that caused post harvest diseases of papaya as compared to uncoated and coated papaya fruits with edible coating without SNPs. (author)

  7. An analysis on DNA fingerprints of thirty papaya cultivars (Carica papaya L.), grown in Thailand with the use of amplified fragment length polymorphisms technique.

    Science.gov (United States)

    Ratchadaporn, Janthasri; Sureeporn, Katengam; Khumcha, U

    2007-09-15

    The experiment was carried out at the Department of Horticulture, Ubon Ratchathani University, Ubon Ratchathani province, Northeast Thailand during June 2002 to May 2003 aims to identify DNA fingerprints of thirty papaya cultivars with the use of Amplified Fragment Length Polymorphisms (AFLP) technique. Papaya cultivars were collected from six different research centers in Thailand. Papaya plants of each cultivar were grown under field conditions up to four months then leaf numbers 2 and 3 of each cultivar (counted from top) were chosen for DNA extraction and the samples were used for AFLP analysis. Out of 64 random primers being used, 55 pairs gave an increase in DNA bands but only 12 pairs of random primers were randomly chosen for the final analysis of the experiment. The results showed that AFLP markers gave Polymorphic Information Contents (PIC) of three ranges i.e., AFLP markers of 235 lied on a PIC range of 0.003-0.05, 47 for a PIC range of 0.15-0.20 and 12 for a PIC range of 0.35-0.40. The results on dendrogram cluster analysis revealed that the thirty papaya cultivars were classified into six groups i.e., (1) Kaeg Dum and Malador (2) Kaeg Nuan (3) Pakchong and Solo (4) Taiwan (5) Co Coa Hai Nan and (6) Sitong. Nevertheless, in spite of the six papaya groups all papaya cultivars were genetically related to each other where diversity among the cultivars was not significantly found. PMID:19090101

  8. An Analysis on DNA Fingerprints of Thirty Papaya Cultivars (Carica papaya L., Grown in Thailand with the Use of Amplified Fragment Length Polymorphisms Technique

    Directory of Open Access Journals (Sweden)

    Janthasri Ratchadaporn

    2007-01-01

    Full Text Available The experiment was carried out at the Department of Horticulture, Ubon Ratchathani University, Ubon Ratchathani province, Northeast Thailand during June 2002 to May 2003 aims to identify DNA fingerprints of thirty papaya cultivars with the use of Amplified Fragment Length Polymorphisms (AFLP technique. Papaya cultivars were collected from six different research centers in Thailand. Papaya plants of each cultivar were grown under field conditions up to four months then leaf numbers 2 and 3 of each cultivar (counted from top were chosen for DNA extraction and the samples were used for AFLP analysis. Out of 64 random primers being used, 55 pairs gave an increase in DNA bands but only 12 pairs of random primers were randomly chosen for the final analysis of the experiment. The results showed that AFLP markers gave Polymorphic Information Contents (PIC of three ranges i.e., AFLP markers of 235 lied on a PIC range of 0.003-0.05, 47 for a PIC range of 0.15-0.20 and 12 for a PIC range of 0.35-0.40. The results on dendrogram cluster analysis revealed that the thirty papaya cultivars were classified into six groups i.e., (1 Kaeg Dum and Malador (2 Kaeg Nuan (3 Pakchong and Solo (4 Taiwan (5 Co Coa Hai Nan and (6 Sitong. Nevertheless, in spite of the six papaya groups all papaya cultivars were genetically related to each other where diversity among the cultivars was not significantly found.

  9. Para una entrada en la imaginacin potica alimentaria chilena

    Scientific Electronic Library Online (English)

    Magda, Seplveda.

    2014-04-01

    Full Text Available Las comidas y las bebidas recreadas en la poesa chilena ofrecen un espacio alternativo para reflexionar sobre la etnia, la clase social, el gnero y la violencia poltica. Cada uno de estos nudos define una ruta alimenticia donde las historias diferenciales se toman la palabra. Este artculo propon [...] e una cartografa de las imgenes alimentarias poticas, caracterizando cuatro grandes rutas: aquellas donde la escritura privilegia la construccin de comunidades indgenas alrededor de la comida y aquellas donde los proyectos estticos elaboran un patrimonio provinciano y/o rural alrededor de las comidas como defensa frente a las legitimidades centristas. Ms aquellos itinerarios cuyas simbolizaciones erigen o deconstruyen los deseos canbales entre diversas subjetividades y; aquellas donde los linajes lingsticos hacen del hambre un motivo de descontento poltico, ya sea imaginando la abundancia a travs del tpico de la tierra de Jauja o elaborando el hambre de los dioses que piden sacrificios humanos. Abstract in english Food and drink recreated in the Chile an poetry offer an alternative space to reflect upon ethnic matters, social class, gender and political violence. Every topoi defines a food route where stories have their say. In this way, we find four main routes: those in which writing priviledges the constru [...] ction of native communities around food preparation; those in which aesthetical projects elaborate their rural patrimony around food to defend themselves from centralized domination; those whose symbols construct and deconstruct cannibalistic desires among different intersubjectivities and those whose linguistic ancestry make hunger a motif of political discontent either by imagining abundance in the land of milk and honey or by elaborating the hunger of gods who demand human sacrifices.

  10. Estudo e modelagem da cintica de desidratao osmtica do mamo formosa (Carica papaya L.) / Kinetics and modeling on osmotic dehydration of papaya (Carica papaya L.)

    Scientific Electronic Library Online (English)

    noar Abbas, El-Aquar; Fernanda E. Xidieh, Murr.

    2003-04-01

    Full Text Available O presente trabalho teve como objetivo o estudo da cintica e modelagem do processo de desidratao osmtica de cubos de mamo Formosa (Carica papaya L.), assim como da qualidade do produto final. O tratamento osmtico foi conduzido a 30C, com agitao de 110rpm, utilizando-se dois tipos de solue [...] s de sacarose 70Brix: a primeira contendo lactato de sdio 2,4% p/p e cido lctico 0,1M e a segunda com lactato de sdio 2,4% p/p e cido ctrico 0,1M. O estudo da cintica de desidratao osmtica mostrou que a soluo contendo cido ctrico apresentou valor de perda de gua (WL), ao final de 48 horas de processo, ligeiramente superior ao encontrado para a soluo contendo cido lctico. Comportamento contrrio ocorreu para o ganho de slidos (SG). O ajuste dos dados experimentais foi realizado atravs do modelo difusional para geometria cbica, sem considerar encolhimento durante o processo e um modelo emprico de dois parmetros. Os coeficientes de difusividade efetiva de gua (Def) variaram de 1,27 a 5,03 x 10-10 m/s. A qualidade da fruta aps processamento foi avaliada atravs de anlises de vitamina C, carotenides totais, acidez e pH. Abstract in english The present work had as objective the study of kinetics and mathematical modeling of osmotic dehydration process of papaya cubes (Carica papaya L.), as well as the quality of the final product. The osmotic treatment was carried out for 30C, with agitation 110rpm, with two types of 70Brix sucrose s [...] olutions: solution containing sodium lactate 2,4% w/w and lactic acid 0,1M and another one with sodium lactate 2,4% w/w and citric acid 0,1M. The study of kinetics of osmotic dehydration showed the solution containing citric acid had a water loss (WL) value, at the end of 48 hours of process, lightly superior to the found for the solution containing lactic acid. Opposite behavior happened for the solid gain (SG). Modeling of experimental data was accomplished through the difusional model for cubic geometry, considering no shrinkage during the process and an empirical two parameters one. The coefficients of effective difusivity of water (Def) varied from 1,27 to 5,03 x 10-10 m/s. Quality of fruit after processing was evaluated through vitamin C, total carotenoid, acidity and pH analyses.

  11. Starch edible coating of papaya: effect on sensory characteristics

    Directory of Open Access Journals (Sweden)

    Ariane Castricini

    2012-03-01

    Full Text Available The coating of papayas with Cassava Starch (CS and carboxymethyl starch (CMS is an alternative to extend the shelf life of these fruits. This study evaluated the effect of the three different levels of CS and CMS (1, 3, and 5% on sensory characteristics of papayas during storage. Nine selected and trained assessors evaluated 13 sensory attributes using the Multiple Comparison Test. The appearance and flavor attributes of the papayas treated with CS and CMS were compared to the control or reference sample (R - fruit without coating using a nine-point scale, which varied from 1: less intense than R; 5: equal to R; 9: more intense than R. The samples were coded with three digit numbers and evaluated with repetition by a panel of assessors. In general, appearance was more affected by the coatings than flavor. Fruits coated with 3 and 5% of both coatings kept the green color longer than the other coating’s concentrations, and at 5% the color of the fruits was less uniform on the last evaluation day. The 3 and 5% CS coating gave greater brightness to the fruits. 5% CMS favored the presence of fungi and damaged the fruit surface at the 14th day of storage. The CS coating at 5% presented peeled surface during all experimental time. Changes in fruits’ flavor were perceived at the 12th and 14th days of storage. A less characteristic flavor and a bitter taste were noticed in the fruits coated with CS and CMS at 5% at the 12th day of storage.

  12. Papaya Extract to Treat Dengue: A Novel Therapeutic Option?

    OpenAIRE

    N. Sarala; Paknikar, SS

    2014-01-01

    Dengue is a viral disease that today affects a vast number of people in over 125 countries and is responsible for a sizable number of deaths. In the absence of an effective antiviral drug to treat the disease, various treatments are being investigated. Studies have indicated that the juice of the leaves of the Carica papaya plant from the family Caricaceae could help to increase the platelet levels in these patients. This review describes some of the published studies on this topic. The searc...

  13. Bioactivity of Carica papaya (Caricaceae) against Spodoptera frugiperda (Lepidoptera: Noctuidae)

    OpenAIRE

    Norma Cecilia Cárdenas-Ortega; Miguel Angel Ramos-López; Miguel Angel Zavala-Sánchez; Marco Martín González-Chávez; Salud Pérez-Gutiérrez

    2011-01-01

    The composition of a chloroform seed extract of C. papaya was determined by GC-MS. Nineteen compounds were identified, with oleic (45.97%), palmitic (24.1%) and stearic (8.52%) acids being the main components. The insecticidal and insectistatic activities of the extract and the three main constituents were tested. Larval duration increased by 3.4 d and 2.5 d when the extract was used at 16,000 and 9,600 ppm, respectively, whereas the pupal period increased by 2.2 d and 1.1 d at the same conce...

  14. Relaxation phenomena of radicals induced in irradiated fresh papayas

    International Nuclear Information System (INIS)

    Electron spin resonance spectrometry of the γ-irradiated fresh papayas followed by freeze-drying and powderization was performed. We found a strong single peak in the flesh was observed at g=2.004 and attributed to organic free radicals. Using the method of Lund et al., relaxation times of the peak from 0 to 14 days-stored samples after γ-irradiation were calculated. T2 showed a dose response, while T1 kept almost constant by the increment of doses. The γ-radiation-induced radicals showing progressive saturation behaviors can be caused through a different pathway from indirect effects by the low LET radiations. (author)

  15. Effects of Edible Micronized Chitosan Coating on Quality and Shelf Life of Sliced Papaya

    OpenAIRE

    Min-Sheng Su; Hung-Ren Lin; Po-Jung Chien

    2013-01-01

    Papaya pulp is very perishable and has a short shelf life. Manually sliced papayas were treated with 0%, 0.25%, 0.5%, and 1% chitosan (non-micronized and micronized) aqueous solutions; placed into plastic trays, and over-wrapped with PVDC film and then stored at 4°C. Color, soluble solid content, water loss, and total plate count of samples were evaluated. Chitosan coating had the ability to maintain the lightness of the sliced papayas. The a* values of the micronized chitosan-coated sliced ...

  16. A Current Overview of the Papaya meleira virus, an Unusual Plant Virus

    OpenAIRE

    Abreu, Paolla M. V.; Tathiana F. S. Antunes; Anuar Magaña-Álvarez; Daisy Pérez-Brito; Raúl Tapia-Tussell; JOSÉ A. VENTURA; Fernandes, Antonio A. R.; Fernandes, Patricia M.B.

    2015-01-01

    Papaya meleira virus (PMeV) is the causal agent of papaya sticky disease, which is characterized by a spontaneous exudation of fluid and aqueous latex from the papaya fruit and leaves. The latex oxidizes after atmospheric exposure, resulting in a sticky feature on the fruit from which the name of the disease originates. PMeV is an isometric virus particle with a double-stranded RNA (dsRNA) genome of approximately 12 Kb. Unusual for a plant virus, PMeV particles are localized on and linked to ...

  17. Papaya pulp gelling: is it premature ripening or problems of water accumulation in the apoplast?

    Directory of Open Access Journals (Sweden)

    Jurandi Gonçalves de Oliveira

    2010-12-01

    Full Text Available Gelled aspect in papaya fruit is typically confused with premature ripening. This research reports the characterization of this physiological disorder in the pulp of papaya fruit by measuring electrolyte leakage, Pi content, lipid peroxidation, pulp firmness, mineral contents (Ca, Mg and K - in pulp and seed tissues, and histological analysis of pulp tissue. The results showed that the gelled aspect of the papaya fruit pulp is not associated with tissue premature ripening. Data indicate a reduction of the vacuole water intake as the principal cause of the loss of cellular turgor; while the waterlogged aspect of the tissue may be due to water accumulation in the apoplast.

  18. SIGNIFICADO DE LA HISTERECTOMA PARA UN GRUPO DE MUJERES CHILENAS

    Directory of Open Access Journals (Sweden)

    Mara Teresa Urrutia S

    2009-01-01

    Full Text Available Antecedentes: La histerectoma por patologa benigna del tero, es una de las cirugas ms frecuente despus de la operacin cesrea. Objetivo: Evaluar el significado que tiene la histerectoma para mujeres chilenas, beneficiaras de un hospital pblico de la Regin Metropolitana de Santiago, Chile. Mtodo: Estudio transversal descriptivo, de tipo cualitativo, sobre el significado que 120 mujeres histerectomizadas asigna a esa ciruga. Se describen 3 temas que reflejan el significado de la extraccin del tero: prdida, tristeza-dolor, y mejora de una enfermedad. Resultados: El promedio de edad fue 56 11,9 aos, y de escolaridad de 7,8 4,3 aos. El 63% de las mujeres fue histerectomizada por patologa ginecolgica benigna. El significado que la mujer atribuye a la extraccin del tero se encuentra relacionado a una experiencia de prdida, que si bien conlleva un alivio de la enfermedad, tambin implica dolor, tristeza y soledad. Conclusin: Sensibilizar a los profesionales de la salud, que trabajan con mujeres histerectomizadas, a comprender y respetar el significado que para ellas tiene la histerectoma y propiciar el ambiente adecuado para contenerlas y educarlas pre y pos ciruga.Background: Elective hysterectomy, after caesarian section, is one of the most frequent surgeries. Objective: To evaluate the significance that the hysterectomy has for Chilean women from a public hospital of the Metropolitan Region of Santiago, Chile. Method: Qualitative and cross-sectional design study of the significance of hysterectomy for 120 women. Three themes were derived from interviews: loss, sadness - sorrow, recovery from illness. Results: The mean of age was 56 11.9 years; the educational level was 7.8 4.3 years. A total of 63% of women had planned elective hysterectomy preformed. The significance that the women attribute to the hysterectomy is related to the experience of loss, and although it includes recovery from illness, it also signifies sorrow, sadness, and loneliness. Conclusion: The health care providers, who work with these women, need to be respectful and understanding of the significance that the women give to this surgery, and to give them the best setting for supporting and educating them before and after the surgery.

  19. The treatment of paediatric burns using topical papaya.

    Science.gov (United States)

    Starley, I F; Mohammed, P; Schneider, G; Bickler, S W

    1999-11-01

    Due to the limited resources for the management of burns in most regions of Africa there is a significant role for many aspects of traditional African medicine. The active component of many traditional preparations is often of plant origin and more than 25 plants have been described as useful in relations to burns and wound healing. Carica papaya is currently used in The Gambia at the Royal Victoria Hospital, Banjul in the Paediatric Unit as the major component of burns dressings, where it is well tolerated by the children. Cheap and widely available, the pulp of the papaya fruit is mashed and applied daily to full thickness and infected burns. It appears to be effective in desloughing necrotic tissue, preventing burn wound infection, and providing a granulating wound suitable for the application of a split thickness skin graft. Possible mechanisms of action include the activity of proteolytic enzymes chymopapain and papain, as well as an antimicrobial activity, although further studies are required. PMID:10563690

  20. Papaya latex enzymes capable of detoxification of gliadin.

    Science.gov (United States)

    Cornell, H J; Doherty, W; Stelmasiak, T

    2010-01-01

    Assay of fractions obtained from ion exchange chromatography of papaya latex on CM Sephadex-C50, size exclusion chromatography on Sephacryl S-300 and size exclusion HPLC have provided an insight into the relative contributions of the gluten-detoxifying enzymes present. This outcome has been achieved by the use of the above chromatographic techniques, coupled with assays of lysosomal activity, protease activity using benzylarginine ethyl ester (BAEE) as substrate, prolyl endopeptidase (PEP) using glycylprolylnitroanilide and a prolidase assay using acetylprolylglycine. These procedures have shown that the activity in papaya latex is due largely to caricain and to a lesser extent, chymopapain and glutamine cyclotransferase. The presence of caricain and these other enzymes was confirmed by mass spectrometry of trypsin digests of the most active fraction obtained by CM Sephadex-C50 chromatography and size exclusion HPLC. Fractions rich in caricain would be suitable for enzyme therapy in gluten intolerance and appear to have synergistic action with porcine intestinal extracts. PMID:19156482

  1. Development of virus resistant transgenic papayas expressing the coat protein gene from a Brazilian isolate of Papaya ringspot virus / Desenvolvimento de mamoeiros transgnicos resistentes a vrus expressando o gene da capa protica de um isolado brasileiro de Papaya ringspot virus

    Scientific Electronic Library Online (English)

    Manoel T., Souza Jnior; Osmar, Nickel; Dennis, Gonsalves.

    2005-08-01

    Full Text Available Verses traduzveis e no traduzveis do gene da capa protica (cp) de um isolado de Papaya ringspot virus (PRSV) coletado no Estado da Bahia, Brasil, foram produzidas para expresso nas variedades Sunrise e Sunset Solo de mamoeiro (Carica papaya). O sistema de biobalstica foi utilizado para transf [...] ormar embries somticos secundrios derivados de embries zigticos imaturos. Cinqenta e quatro linhas transgnicas, sendo 26 contendo verses traduzveis e 28 contendo verses no traduzveis do gene cp foram regeneradas, o que resultou em 2,7% de eficincia de transformao, quando considerado o nmero de linhas transgnicas obtidas por embrio zigtico imaturo excisado. Desafios de plantas R0 com PRSV BR, PRSV HA ou PRSV TH, respectivamente isolado brasileiro, havaiano e tailands, revelaram linhas com resistncia a um, dois e trs isolados de PRSV. Aps anlises moleculares e avaliao agronmica preliminar, 13 populaes R1 e R2 de mamoeiros transgnicos foram incorporadas ao programa de melhoramento gentico da Embrapa Mandioca e Fruticultura, em Cruz das Almas, Bahia, Brasil. Abstract in english Translatable and nontranslatable versions of the coat protein (cp) gene of a Papaya ringspot virus (PRSV) isolate collected in the state of Bahia, Brazil, were engineered for expression in Sunrise and Sunset Solo varieties of papaya (Carica papaya). The biolistic system was used to transform seconda [...] ry somatic embryo cultures derived from immature zygotic embryos. Fifty-four transgenic lines, 26 translatable and 28 nontranslatable gene versions, were regenerated, with a transformation efficiency of 2.7%. Inoculation of cloned R0 plants with PRSV BR, PRSV HA or PRSV TH, Brazilian, Hawaiian and Thai isolates, respectively, revealed lines with mono-, double-, and triple-resistance. After molecular analysis and a preliminary agronomic evaluation, 13 R1 and R2 populations were incorporated into the papaya-breeding program at Embrapa Cassava and Tropical Fruits, in Cruz das Almas, Bahia, Brazil.

  2. Development of virus resistant transgenic papayas expressing the coat protein gene from a Brazilian isolate of Papaya ringspot virus Desenvolvimento de mamoeiros transgnicos resistentes a vrus expressando o gene da capa protica de um isolado brasileiro de Papaya ringspot virus

    Directory of Open Access Journals (Sweden)

    Manoel T. Souza Jnior

    2005-08-01

    Full Text Available Translatable and nontranslatable versions of the coat protein (cp gene of a Papaya ringspot virus (PRSV isolate collected in the state of Bahia, Brazil, were engineered for expression in Sunrise and Sunset Solo varieties of papaya (Carica papaya. The biolistic system was used to transform secondary somatic embryo cultures derived from immature zygotic embryos. Fifty-four transgenic lines, 26 translatable and 28 nontranslatable gene versions, were regenerated, with a transformation efficiency of 2.7%. Inoculation of cloned R0 plants with PRSV BR, PRSV HA or PRSV TH, Brazilian, Hawaiian and Thai isolates, respectively, revealed lines with mono-, double-, and triple-resistance. After molecular analysis and a preliminary agronomic evaluation, 13 R1 and R2 populations were incorporated into the papaya-breeding program at Embrapa Cassava and Tropical Fruits, in Cruz das Almas, Bahia, Brazil.Verses traduzveis e no traduzveis do gene da capa protica (cp de um isolado de Papaya ringspot virus (PRSV coletado no Estado da Bahia, Brasil, foram produzidas para expresso nas variedades Sunrise e Sunset Solo de mamoeiro (Carica papaya. O sistema de biobalstica foi utilizado para transformar embries somticos secundrios derivados de embries zigticos imaturos. Cinqenta e quatro linhas transgnicas, sendo 26 contendo verses traduzveis e 28 contendo verses no traduzveis do gene cp foram regeneradas, o que resultou em 2,7% de eficincia de transformao, quando considerado o nmero de linhas transgnicas obtidas por embrio zigtico imaturo excisado. Desafios de plantas R0 com PRSV BR, PRSV HA ou PRSV TH, respectivamente isolado brasileiro, havaiano e tailands, revelaram linhas com resistncia a um, dois e trs isolados de PRSV. Aps anlises moleculares e avaliao agronmica preliminar, 13 populaes R1 e R2 de mamoeiros transgnicos foram incorporadas ao programa de melhoramento gentico da Embrapa Mandioca e Fruticultura, em Cruz das Almas, Bahia, Brasil.

  3. Efficacy of Essential Oils on the Conidial Germination, Growth of Colletotrichum gloeosporioides (Penz. Penz. and Sacc and Control of Postharvest Diseases in Papaya (Carica papaya L.

    Directory of Open Access Journals (Sweden)

    Laura Leticia Barrera-Necha

    2008-01-01

    Full Text Available The efficacy of nine essential oils were investigated in vitro on conidial germination and mycelial growth inhibition of Colletotrichum gloeosporioides isolate from papaya (Carica papaya L.. In general, better antifungal effect was observed with Cinnamomum zeylanicum and Syzygium aromaticum oils which had strong inhibition of conidial germination of C. gloeosporioides at 50, 100, 150, 200 and 250 μg mL-1 and a dose dependent inhibition mycelial growth was caused by these oils. Teloxys ambrosioides, Mentha piperita and Ruta chalepensis oils exhibited a moderate action at 150, 200 and 250 μg mL-1 on conidial germination and mycelial growth inhibition. Allium sativum, Citrus aurantifolia and Eucalyptus globulus oils had no antifungal activity at different concentration. Taking into account the in vitro results, C. zeylanicum and S. aromaticum oils were evaluated on papaya fruit during storage at ambient temperature and 14°C. The lowest infection percentage were for papaya fruits treated with S. aromaticum at 50 μg mL-1 at both temperature tested, nevertheless did not overcome the activity of synthetic fungicide. After storage at both temperature, values of Soluble Solids Content (SSC was not significantly different. S. aromaticum oils may be a possibility to control C. gloeosporioides of papaya fruit.

  4. Immobilization of mycoplana sp. MVMB2 isolated from petroleum contaminated soil onto papaya stem (carica papaya l.) and its application on degradation of phenanthrene

    Energy Technology Data Exchange (ETDEWEB)

    Brinda Lakshmi, Mahalingam; Muthukumar, Karuppan; Velan, Manickam [Environmental Biotechnology Laboratory, Department of Chemical Engineering, A.C. College of Technology, Anna University, Chennai (India)

    2012-08-15

    This study presents the degradation of phenanthrene using immobilized Mycoplana sp. MVMB2 isolated from contaminated soil. Papaya stem pretreated by two stage processes, treating with acid or alkali and drying, was used for the immobilization of Mycoplana sp. Alkali pretreated papaya stem was found to be most effective in cell uptake compared to acid treated one. The maximum immobilization capacity at various physiochemical conditions for the alkali pretreated papaya stem was found to be at 320 min time, pH 6.5, 30 C temperature, and 18.6 x 10{sup 6} cells/mL initial concentrations. The adsorption mechanism of Mycoplana sp. MVMB2 on pretreated papaya stem was assessed using various kinetic and isotherm models. The immobilization of Mycoplana sp. MVMB2 on to pretreated papaya stem was corroborated by scanning electron microscopy and Fourier transformed IR spectroscopy analysis. The performance of immobilized cells in batch reactor showed more than 95% phenanthrene degradation within 72 h, whereas, free cells were found to require 120 h. The immobilized cells also showed better degradation performance in the packed column study. (Copyright copyright 2012 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  5. Immobilization of mycoplana sp. MVMB2 isolated from petroleum contaminated soil onto papaya stem (carica papaya l.) and its application on degradation of phenanthrene

    International Nuclear Information System (INIS)

    This study presents the degradation of phenanthrene using immobilized Mycoplana sp. MVMB2 isolated from contaminated soil. Papaya stem pretreated by two stage processes, treating with acid or alkali and drying, was used for the immobilization of Mycoplana sp. Alkali pretreated papaya stem was found to be most effective in cell uptake compared to acid treated one. The maximum immobilization capacity at various physiochemical conditions for the alkali pretreated papaya stem was found to be at 320 min time, pH 6.5, 30 C temperature, and 18.6 x 106 cells/mL initial concentrations. The adsorption mechanism of Mycoplana sp. MVMB2 on pretreated papaya stem was assessed using various kinetic and isotherm models. The immobilization of Mycoplana sp. MVMB2 on to pretreated papaya stem was corroborated by scanning electron microscopy and Fourier transformed IR spectroscopy analysis. The performance of immobilized cells in batch reactor showed more than 95% phenanthrene degradation within 72 h, whereas, free cells were found to require 120 h. The immobilized cells also showed better degradation performance in the packed column study. (Copyright copyright 2012 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  6. Screening of papaya accessions resistant to Papaya lethal yellowing virus and capacity of Tetranychus urticae to transmit the virus / Identificao de acessos de mamoeiro resistentes ao Papaya lethal yellowing virus e capacidade de Tetranychus urticae em transmitir o vrus

    Scientific Electronic Library Online (English)

    Marcos Fernando, Basso; lvaro Jlio, Pereira; Hermano Monteiro de Barros, Pereira; Humberto Josu de Oliveira, Ramos; Jorge Luiz Loyola, Dantas; Elizabeth Pacheco Batista, Fontes; Eduardo Chumbinho de, Andrade; Francisco Murilo, Zerbini.

    2015-02-01

    Full Text Available O objetivo deste trabalho foi produzir um antissoro policlonal contra a protena capsidial (PC) do Papaya lethal yellowing virus (PLYV) e determinar sua especificidade e sensibilidade na diagnose do vrus, bem como avaliar a resistncia gentica de acessos de mamoeiro (Carica papaya) ao PLYV e inves [...] tigar a capacidade do caro rajado Tetranychus urticae em adquirir e transmitir o vrus s plantas. Foram avaliados 65 acessos de mamoeiro. Para cada acesso, dez plantas foram submetidas inoculao mecnica com extratos de plantas infectadas com PLYV, e trs plantas receberam inoculao apenas com tampo de fosfato e foram usadas como controle negativo. Noventa dias aps a inoculao, novas folhas sistmicas emergentes foram coletadas das plantas inoculadas, e a infeco viral foi diagnosticada por Elisa indireto, com uso de antissoro policlonal sensvel PC do PLYV expressa in vitro. A transmisso viral por T. urticae foi avaliada em casa de vegetao. Os experimentos foram repetidos duas vezes. O antissoro policlonal reconheceu a PC do PLYV especificamente e discriminou a infeco pelo PLYV de infeces causadas por outros vrus. Dos 65 acessos de mamoeiros avaliados, 15 foram considerados resistentes, 18 moderadamente resistentes e 32 suscetveis. O caro rajado T. urticae foi capaz de adquirir o PLYV, mas no de transmiti-lo para o mamoeiro. Abstract in english The objective of this work was to produce a polyclonal antiserum against the coat protein (CP) of Papaya lethal yellowing virus (PLYV) and to determine its specificity and sensibility in the diagnosis of the virus, as well as to evaluate the genetic resistance to PLYV in papaya (Carica papaya) acces [...] sions and to investigate the capacity of the two-spotted spider mite Tetranychus urticae to acquire and transmit PLYV to the plants. Sixty-five papaya accessions were evaluated. For each accession, ten plants were mechanically inoculated using PLYV-infected plant extracts, and three plants were mock inoculated with phosphate buffer alone and used as negative controls. Ninety days after inoculation, newly-emerging systemic leaves were collected from the inoculated plants, and viral infection was diagnosed by indirect Elisa, using polyclonal antiserum sensible to the in vitro-expressed PLYV CP. Viral transmission by T. urticae was evaluated in greenhouse. The experiments were repeated twice. Polyclonal antiserum recognized the recombinant PLYV CP specifically and discriminated PLYV infection from infections caused by other plant viruses. Out of the 65 papaya accessions evaluated, 15 were considered resistant, 18 moderately resistant, and 32 susceptible. The two-spotted spider mite T. urticae was capable of acquiring PLYV, but not of transmitting it to papaya.

  7. In vitro erythrocyte membrane stabilization properties of Carica papaya L. leaf extracts

    Directory of Open Access Journals (Sweden)

    Priyanga Ranasinghe

    2012-01-01

    Full Text Available Background : Carica papaya L. fruit juice and leaf extracts are known to have many beneficial medical properties. Recent reports have claimed possible beneficial effects of C. papaya L. leaf juice in treating patients with dengue viral infections. This study aims to evaluate the membrane stabilization potential of C. papaya L. leaf extracts using an in vitro hemolytic assay. Materials and Methods: The study was conducted in between June and August 2010. Two milliliters of blood from healthy volunteers and patients with serologically confirmed current dengue infection were freshly collected and used in the assays. Fresh papaya leaves at three different maturity stages (immature, partly matured, and matured were cleaned with distilled water, crushed, and the juice was extracted with 10 ml of cold distilled water. Freshly prepared cold water extracts of papaya leaves (1 ml containing 30 μl of papaya leaf extracts, 20 μl from 40% erythrocytes suspension, and 950 μl of phosphate buffered saline were used in the heat-induced and hypotonic-induced hemolytic assays. In dose response experiments, six different concentrations (9.375, 18.75, 37.5, 75, 150, and 300 μg/ml of freeze dried extracts of the partly matured leaves were used. Membrane stabilization properties were investigated with heat-induced and hypotonicity-induced hemolysis assays. Results: Extracts of papaya leaves of all three maturity levels showed a significant reduction in heat-induced hemolysis compared to controls (P 0.05 different from one another. Heat-induced hemolysis inhibition activity did not demonstrate a linear dose response relationship. At 37.5 μg/ml concentration of the extract, a marked inhibition of hypotonicity-induced hemolysis was observed. Conclusion: C. papaya L. leaf extracts showed a significant inhibition of hemolysis in vitro and could have a potential therapeutic effect on disease processes causing destabilization of biological membranes.

  8. Carica papaya induces in vitro thrombopoietic cytokines secretion by mesenchymal stem cells and haematopoietic cells

    OpenAIRE

    Aziz, Jazli; Abu Kassim, Noor Lide; Abu Kasim, Noor Hayaty; Haque, Nazmul; Rahman, Mohammad Tariqur

    2015-01-01

    Background Use of Carica papaya leaf extracts, reported to improve thrombocyte counts in dengue patients, demands further analysis on the underlying mechanism of its thrombopoietic cytokines induction Methods In vitro cultures of peripheral blood leukocytes (PBL) and stem cells from human exfoliated deciduous teeth (SHED) were treated with unripe papaya pulp juice (UPJ) to evaluate its potential to induce thrombopoietic cytokines (IL-6 and SCF) Results In vitro scratch gap closure was signifi...

  9. Antifungal Activity in Ethanolic Extracts of Carica papaya L. cv. Maradol Leaves and Seeds

    OpenAIRE

    Chávez-Quintal, Pedro; González-Flores, Tania; Rodríguez-Buenfil, Ingrid; Gallegos-Tintoré, Santiago

    2011-01-01

    Bioactive compounds from vegetal sources are a potential source of natural antifungic. An ethanol extraction was used to obtain bioactive compounds from Carica papaya L. cv. Maradol leaves and seeds of discarded ripe and unripe fruit. Both, extraction time and the papaya tissue flour:organic solvent ratio significantly affected yield, with the longest time and highest flour:solvent ratio producing the highest yield. The effect of time on extraction efficiency was confirmed by qualitative iden...

  10. Hypoglycemic effect of Carica papaya leaves in streptozotocin-induced diabetic rats

    OpenAIRE

    Juárez-Rojop Isela Esther; Díaz-Zagoya Juan C; Ble-Castillo Jorge L; Miranda-Osorio Pedro H; Castell-Rodríguez Andrés E; Tovilla-Zárate Carlos A; Rodríguez-Hernández Arturo; Aguilar-Mariscal Hidemi; Ramón-Frías Teresa; Bermúdez-Ocaña Deysi Y

    2012-01-01

    Abstract Background Traditional plant treatment for diabetes has shown a surging interest in the last few decades. Therefore, the purpose of this study was to assess the hypoglycemic effect of the aqueous extract of C. papaya leaves in diabetic rats. Several studies have reported that some parts of the C. papaya plant exert hypoglycemic effects in both animals and humans. Methods Diabetes was induced in rats by intraperitoneal administration of 60 mg/kg of streptozotocin (STZ). The aqueous ex...

  11. Biodiesel Production by Enzymatic Transesterification of Papaya Seed Oil and Rambutan Seed Oil

    OpenAIRE

    Wong, C S; R. Othman

    2014-01-01

    Biodiesel production from vegetable oil has gained attention as an alternative fuel to minimize the usage of fossil fuels and reduce greenhouse gases pollution. In Malaysia, oils from local fruit seeds of papaya and rambutan are potential feedstock for biodiesel production due to their high lipid contents and easily available. In the present study, papaya and rambutan seed oils were extracted via soxhlet apparatus using n-hexane and the oil yields were in between 34–40%. The extracted oils we...

  12. Compositional difference in antioxidant and antibacterial activity of all parts of the Carica papaya using different solvents

    OpenAIRE

    Asghar, Nazia; Naqvi, Syed Ali Raza; Hussain, Zaib; Rasool, Nasir; Khan, Zulfiqar Ali; Shahzad, Sohail Anjum; Sherazi, Tauqir A.; Janjua, Muhammad Ramzan Saeed Ashraf; Nagra, Saeed Ahmad; Zia-Ul-Haq, Muhammad; Jaafar, Hawa ZE

    2016-01-01

    Background Carica papaya is a well known medicinal plant used in the West and Asian countries to cope several diseases. Patients were advised to eat papaya fruit frequently during dengue fever epidemic in Pakistan by physicians. This study was conducted to establish Polyphenols, flavonoids and antioxidant potential profile of extracts of all major parts of the C. papaya with seven major solvents i.e. water, ethanol, methanol, n-butanol, dichloromethane, ethyl acetate, and n-hexane. Results TP...

  13. Papain protects papaya trees from herbivorous insects: role of cysteine proteases in latex.

    Science.gov (United States)

    Konno, Kotaro; Hirayama, Chikara; Nakamura, Masatoshi; Tateishi, Ken; Tamura, Yasumori; Hattori, Makoto; Kohno, Katsuyuki

    2004-02-01

    Many plants contain latex that exudes when leaves are damaged, and a number of proteins and enzymes have been found in it. The roles of those latex proteins and enzymes are as yet poorly understood. We found that papain, a cysteine protease in latex of the Papaya tree (Carica papaya, Caricaceae), is a crucial factor in the defense of the papaya tree against lepidopteran larvae such as oligophagous Samia ricini (Saturniidae) and two notorious polyphagous pests, Mamestra brassicae (Noctuidae) and Spodoptera litura (Noctuidae). Leaves of a number of laticiferous plants, including papaya and a wild fig, Ficus virgata (Moraceae), showed strong toxicity and growth inhibition against lepidopteran larvae, though no apparent toxic factors from these species have been reported. When the latex was washed off, the leaves of these lactiferous plants lost toxicity. Latexes of both papaya and the wild fig were rich in cysteine-protease activity. E-64, a cysteine protease-specific inhibitor, completely deprived the leaves of toxicity when painted on the surface of papaya and fig leaves. Cysteine proteases, such as papain, ficin, and bromelain, all showed toxicity. The results suggest that plant latex and the proteins in it, cysteine proteases in particular, provide plants with a general defense mechanism against herbivorous insects. PMID:14731257

  14. Oral dosing with papaya latex is an effective anthelmintic treatment for sheep infected with Haemonchus contortus

    Directory of Open Access Journals (Sweden)

    Donnan Alison A

    2011-03-01

    Full Text Available Abstract Background The cysteine proteinases in papaya latex have been shown to have potent anthelmintic properties in monogastric hosts such as rodents, pigs and humans, but this has not been demonstrated in ruminants. Methods In two experiments, sheep were infected concurrently with 5,000 infective larvae of Haemonchus contortus and 10,000 infective larvae of Trichostrongylus colubriformis and were then treated with the supernatant from a suspension of papaya latex from day 28 to day 32 post-infection. Faecal egg counts were monitored from a week before treatment until the end of the experiment and worm burdens were assessed on day 35 post-infection. Results We found that the soluble fraction of papaya latex had a potent in vivo effect on the abomasal nematode H. contortus, but not on the small intestinal nematode T. colubriformis. This effect was dose-dependent and at tolerated levels of gavage with papaya latex (117 μmol of active papaya latex supernatant for 4 days, the H. contortus worm burdens were reduced by 98%. Repeated treatment, daily for 4 days, was more effective than a single dose, but efficacy was not enhanced by concurrent treatment with the antacid cimetidine. Conclusions Our results provide support for the idea that cysteine proteinases derived from papaya latex may be developed into novel anthelmintics for the treatment of lumenal stages of gastro-intestinal nematode infections in sheep, particularly those parasitizing the abomasum.

  15. Gamma radiation use as a quarantine treatment for Ceratitis capitata (Wied., 1824) and Anastrepha fraterculus (Wied., 1830) in papaya fruits (Carica papaya, Linnaeus), cultivar sunrise solo

    International Nuclear Information System (INIS)

    The gamma irradiation as treatment for control of the immature stages of fruit flies, C. capitata and A. fraterculus in papaya fruits, cultivar Sunrise Solo is studied. The gamma rays attenuation was observed by one and two papaya fruits placed between source and infested fruit, and this changed the radiation doses required to obtain mortality in eggs and larvae of the Medfly. The efficacy of irradiation was higher when the infested papayas were packed in paper box. The radiation dose determined using the Probit 9 concept, for immature stages mortality of C. capitata was 107,74 Gy and for A. fraterculus was 50,82 Gy. The sterilizing doses for adults of C. capitata and D. fraterculus, irradiated as pupae, was 60 Gy and 40 Gy, respectively. (author)

  16. Preferential reproduction mode of hermaphrodite papaya plant (Carica papaya L; Caricaceae Modo de reprodução preferencial de plantas hermafroditas de mamoeiro (Carica papaya L; Caricaceae

    Directory of Open Access Journals (Sweden)

    Pedro Corrêa Damasceno junior

    2009-03-01

    Full Text Available This research was done to study the reproductive system of papaya hermaphrodite plant based on the histochemical nature of pollen grain, stigma receptivity, in vivo pollen grain germination and pollen:ovule ratio. In the histochemical analysis, pollen grains were stained by using Sudan IV and I2KI solution ; the stigma receptivity was assessed by alpha-naphthtyl acetate solution in closed and opened flowers. Pollen germination and pollen tube growing were examined in flower buds near anthesis with 0.1% aniline blue. To estimate the pollen:ovule ratio , anthers from each flower bud were dissected and all pollen grains were counted; ovules were dissected from ovaries and were counted under stereomicroscope. The results indicated that papaya pollen grains are of lipidic nature; the stigmas were receptive before the opening and until 48 hours after opening; the pollen grains germinated and emitted polinic tube before flower opening and the pollen:ovule ratio indicated the predominance of autogamous reproductive system. These results indicate that hermaphrodite papaya trees is preferentially of optional autogamous with cleistogamy.Esta pesquisa teve o objetivo de estudar o sistema reprodutivo preferencial de plantas hermafroditas de mamoeiro, com base na natureza histoquímica dos grãos de pólen, receptividade do estigma, teste de germinação in vivo do grão de pólen e razão pólen : óvulo. Na análise histoquímica dos grãos de pólen, foram utilizados os corantes Sudan IV e solução de I2KI; a receptividade do estigma foi avaliada com a solução de acetato de alfa-naftil em flores abertas e fechadas. A germinação e o crescimento do tubo polínico in vivo foram avaliados em flores fechadas, utilizando solução de azul de anilina a 0,1%. Para estimar a razão pólen:óvulo, anteras de cada flor foram dissecadas, e os grãos de pólen foram contados; ovários foram dissecados, e os óvulos foram contados sob estereomicroscópio. Os resultados indicaram que os grãos de pólen do mamoeiro são de natureza lipídica; os estigmas estavam receptivos antes da abertura e até 48 h após a abertura; os grãos de pólen germinaram e emitiram tubo polínico antes da abertura floral, e a relação pólen:óvulo, indicou predominância do sistema reprodutivo autógamo. Esses resultados indicaram que o modo de reprodução preferencial do mamoeiro hermafrodita é autógamo facultativo com cleistogamia.

  17. Las relaciones civiles-militares en el marco de la participacin chilena en operaciones de paz

    Directory of Open Access Journals (Sweden)

    Jorge Riquelme Rivera

    2012-01-01

    Full Text Available El trabajo analiza las relaciones entre civiles y militares en el contexto de la participacin chilena en operaciones de paz, concentrndose especialmente en la Misin de Estabilizacin de Naciones Unidas en Hait (MINUSTAH, establecida el ao 2004. En tal sentido -y considerando la creciente convergencia entre la poltica exterior de Chile y su poltica de defensa-, se sostiene que la participacin chilena en operaciones de paz ha contribuido a la integracin entre civiles y militares, por cuanto ha favorecido una estrategia de trabajo conjunta e integradora entre el Ministerio de Relaciones Exteriores, el Ministerio de Defensa y las fuerzas armadas; as como un estrecho y coordinado trabajo entre los variados actores civiles y militares que laboran en el territorio haitiano.

  18. DISCURSO ACADMICO DE LANZAMIENTO DE LA REVISTA CHILENA DE DERECHO Y CIENCIA POLTICA

    Directory of Open Access Journals (Sweden)

    Patricio - Ignacio Carvajal

    2012-07-01

    Full Text Available Me complace enormemente la invitacin que se me ha cursado para dirigir algunaspalabras en el lanzamiento de esta Revista Chilena de Derecho y Ciencia Poltica.Me honra el hecho de que me hayan tenido Uds. en consideracin para este actoacadmico y, por supuesto, tambin que me hayan incorporado al elenco de especialistasde su Comit Cientfi co Externo. Por ello, comienzo por expresar mi sinceroagradecimiento.

  19. La Economia Chilena Frente a la Crisis Financiera: Respuestas Contra-Ciclicas y Desafios Pendientes

    OpenAIRE

    Ricardo Ffrench-Davis; Rodrigo Heresi

    2014-01-01

    Ante el contagio de la crisis financiera internacional, la economia chilena experimento una intensa reduccion del precio del cobre y de los influjos de capitales. Consecuentemente, la demanda agregada y el empleo de trabajo y capital productivo se contrajeron hacia fines de 2008. En ese escenario, a diferencia de la reaccion frente al contagio de la crisis asiatica, la autoridad economica adopto un amplio conjunto de politicas publicas contra-ciclicas, caracterizadas principalmente por un aum...

  20. poca de colheita dos frutos e ocorrncia de dormncia em sementes de mamo (Carica papaya L. Fruit harvest season and dormancy occurrency in papaya seeds (Carica papaya L.

    Directory of Open Access Journals (Sweden)

    Da Tokuhisa

    2008-01-01

    Full Text Available O trabalho teve como objetivo relacionar a poca de colheita dos frutos com a ocorrncia de dormncia nas sementes de mamo. Foram utilizados frutos de mamo do grupo Formosa, hbrido Tainung 01, colhidos no estdio 1 de maturao (at 15% da superfcie externa com colorao amarela em cinco diferentes pocas do ano: Julho e Novembro/2004 e Fevereiro, Maio e Setembro/2005. Os frutos permaneceram armazenados em ambiente de laboratrio at atingirem o estdio 5 de maturao (mais de 75% da superfcie externa com colorao amarela. Em cada poca, foram obtidas sementes com e sem sarcotesta, que foram submetidas ao teste de germinao, conduzido em germinador sob temperatura alternada de 20-30C (16h/8h, respectivamente, avaliando-se a porcentagem de plntulas normais aos 15 e 30 dias aps a semeadura. Verificou-se que a presena da sarcotesta na semente diminui a velocidade e a porcentagem de germinao. Houve efeito da poca de colheita do fruto na germinao das sementes, sendo que as sementes extradas de frutos colhidos em Julho/2004 e Maio/2005 apresentaram dormncia ps-colheita, o que no ocorreu com as sementes obtidas de frutos que amadureceram em pocas de temperaturas mais altas.This study was carried out in order to relate the fruit harvest season and dormancy occurrence in papaya seeds. Papaya fruits of the Formosa group, Tainung 01 hybrid, were harvested at maturation stage 1 (15% external color yellow, at five different times: July and November/2004 and February, May and September/2005. The fruits were stored at room temperature until they reached maturation stage 5 (more than 75% external color yellow. Seeds with and without sarcotesta were obtained at each harvest time. Standard germination of these seeds was performed in a germinator at 20/30 C (8/16 hours, respectively and normal seedlings percentage was evaluated at 15 and 30 days. Seeds with sarcotesta had lower germination speed and percentage. There was an effect of fruit harvest time on seed germination. Seeds extracted from fruits harvested in July/2004 and May/2005 had post-harvest dormancy, which did not occur in seeds from fruits harvested in the high temperature periods.

  1. Morfología de la flor y de la semilla de papaya (carica papaya l.): variedad maradol e híbrido tainung-1

    OpenAIRE

    Gil, Arlette Ivonne; Miranda, Diego

    2010-01-01

    Con el objetivo de analizar la morfología de la flor y de la semilla de papaya (Carica papaya L.) de la variedad ‘Maradol’ y el híbrido ‘Tainung-1’, se recolectó el material vegetal en dos plantaciones y se llevaron al laboratorio de Fisiología de Cultivos, Facultad de Agronomía, Universidad Nacional de Colombia, Bogotá, con el fin de realizar las descripciones correspondientes de los tres tipos de flores (femeninas, hermafroditas y estaminadas) y de la semilla, sus características externas (...

  2. Heat treatment and gamma radiation on the control of Colletotrichum gloeosporioides (Penz.) Penz. et Sacc., causal agent of the papaya fruits (Carica papaya L.) anthracnose

    International Nuclear Information System (INIS)

    For controlling papaya anthracnose on stored fruits the effect of heat treatment and gamma radiation on Colletotrichum gloeosporioides was studied in vitro and in vivo. Some physiological studies with four isolades of C. gloeosporioides from papaya fruits concerning the best conditions for mycelial growth and sporulation were also performed. The results showed that the best conditions for isolates development in BDA were: temperature between 25 and 270 C for mycelium radial growth; between 27 and 300 C under 12 hours alternating periods of fluorescent). (author). 131 refs., 9 figs., 33 tabs

  3. ESTIMACIÓN DEL ÁREA FOLIAR DE PAPAYA (Carica papaya L. BASADA EN MUESTREO NO DESTRUCTIVO ESTIMATION OF PAPAYA (Carica papaya L. LEAF AREA BASED ON NON-DESTRUCTIVE SAMPLING

    Directory of Open Access Journals (Sweden)

    Carlos Cardona Ayala

    Full Text Available El cultivo de papaya (Carica papaya L. es muy importante en la fruticultura del departamento de Córdoba, por la buena calidad de la fruta producida. La investigación, se realizó en Montería, a 13msnm, temperatura media de 27.4°C, precipitación anual de 1346,1mm, humedad relativa de 84% y brillo solar anual de 2180,2 horas, con el fin de obtener un modelo que permita estimar el área foliar de la planta, a partir de muestreo no destructivo. Mediante muestreo aleatorio simple, se seleccionaron hojas sanas de plantas adultas, de diferentes tamaños y distintas etapas de desarrollo, de los doseles superior, medio e inferior, de la variedad Maradol roja. Se tomaron medidas longitudinales: largo y ancho máximo de la hoja, ancho de la hoja a un medio, un tercio, un cuarto, un quinto y dos tercios de su largo, ancho máximo de lóbulos y longitud de nervadura de los mismos, con la finalidad de determinar las medidas altamente relacionadas con el área fotosintética de la hoja. El área foliar (Y, se midió por el método de relación peso: área, sobre una muestra de 30 hojas por dosel. Se construyeron modelos de regresión simple y múltiple, para luego seleccionar el de mejor ajuste. Se seleccionó un modelo de regresión simple, cuya variable explicativa corresponde a la longitud de la nervadura central del lóbulo izquierdo, contiguo al adyacente lóbulo medio (X. El modelo ajustado resultó ser: o = -303,0742 + 31,2028X, R² = 0,9335.The papaya (Carica papaya L is a very important crop in the Cordoba state, especially for the good fruit quality. This research was carried out at Montería at 13masl, 27.4°C, annual rainfall of 1346.1mm, 84% relative humidity and 2180.2 hours annual sunlight, in order to obtain a model to estimate the plants leaf area, based on non-destructive sampling. A probability sampling procedure was used to select different size leaves and developmental stages from the upper, middle and lower canopy of adult plants of the red Maradol variety. Maximum leaf length and width, width of the middle third, fourth, fifth and two-thirds of leaf length, lobe width and midrib length of every lobe were registered with the purpose of identifying measures highly related to photosynthetic leaf area. The leaf area (Y was measured based on the weight:area relation, from 30 leaves per canopy sample. Simple linear regression models and multiple linear regression were adjusted. A simple linear regression model was selected which independent variable representing the length of the midrib, next to the left next lobe and adjacent to the middle lobe (X. The adjusted model was: or = -303,0742 + 31,2028X, R² = 0,9335.

  4. Identification of gamma-irradiated papaya, melon and watermelon

    International Nuclear Information System (INIS)

    Ionizing radiation can be used to control spoilage microorganisms and to increase the shelf life of fresh fruits and vegetables in replacement for the treatment with chemical fumigants. In order to enforce labelling regulations, methods for detecting the irradiation treatment directly in the produce are required. Recently, a number of detection methods for irradiated food have been adopted by the Codex Comission. A rapid screening method for qualitative detection of irradiation is the DNA Comet Assay. The applicability of the DNA Comet Assay for distinguishing irradiated papaya, melon, and watermelon was evaluated. The samples were treated in a 60Co facility at dose levels of 0.0, 0.5, 0.75, and 1.0 kGy. The irradiated samples showed typical DNA fragmentation whereas cells from non-irradiated ones appeared intact. In addition to the DNA Comet Assay also the half-embryo test was applied in melon and watermelon to detect the irradiation treatment

  5. Identification of gamma-irradiated papaya, melon and watermelon

    Science.gov (United States)

    Marín-Huachaca, Nélida S.; Mancini-Filho, Jorge; Delincée, Henry; Villavicencio, Anna Lúcia C. H.

    2004-09-01

    Ionizing radiation can be used to control spoilage microorganisms and to increase the shelf life of fresh fruits and vegetables in replacement for the treatment with chemical fumigants. In order to enforce labelling regulations, methods for detecting the irradiation treatment directly in the produce are required. Recently, a number of detection methods for irradiated food have been adopted by the Codex Comission. A rapid screening method for qualitative detection of irradiation is the DNA Comet Assay. The applicability of the DNA Comet Assay for distinguishing irradiated papaya, melon, and watermelon was evaluated. The samples were treated in a 60Co facility at dose levels of 0.0, 0.5, 0.75, and 1.0kGy. The irradiated samples showed typical DNA fragmentation whereas cells from non-irradiated ones appeared intact. In addition to the DNA Comet Assay also the half-embryo test was applied in melon and watermelon to detect the irradiation treatment.

  6. Identification of gamma-irradiated papaya, melon and watermelon

    Energy Technology Data Exchange (ETDEWEB)

    Marin-Huachaca, N.S.; Mancini-Filho, Jorge E-mail: jmancini@usp.br; Delincee, Henry E-mail: henry.delincee@bfe.uni-karlsruhe.de; Villavicencio, A.L.C.H. E-mail: villavic@net.ipen.br

    2004-10-01

    Ionizing radiation can be used to control spoilage microorganisms and to increase the shelf life of fresh fruits and vegetables in replacement for the treatment with chemical fumigants. In order to enforce labelling regulations, methods for detecting the irradiation treatment directly in the produce are required. Recently, a number of detection methods for irradiated food have been adopted by the Codex Comission. A rapid screening method for qualitative detection of irradiation is the DNA Comet Assay. The applicability of the DNA Comet Assay for distinguishing irradiated papaya, melon, and watermelon was evaluated. The samples were treated in a {sup 60}Co facility at dose levels of 0.0, 0.5, 0.75, and 1.0 kGy. The irradiated samples showed typical DNA fragmentation whereas cells from non-irradiated ones appeared intact. In addition to the DNA Comet Assay also the half-embryo test was applied in melon and watermelon to detect the irradiation treatment.

  7. Pengaruh Konsentrasi Natrium Klorida (NaCl) dan Lama Perendaman Buffer Fosfat Terhadap Perolehan Crude Papain Dari Daun Papain (Carica Papaya, L.)

    OpenAIRE

    Hasibuan, Pinta Rizki Mala

    2016-01-01

    Papaya (Carica papaya L.) is one of the fruits of commodities internationally, either in the form of fresh fruit or as processed products. The leaves are green still not fully utilized. In papaya enzyme papain which allegedly contained can be used as a meat tenderizer. Papain is a protease enzyme contained in papaya latex, whether in fruit, stems and leaves, as an enzyme capable of solving the protein molecules, current papain into products that are beneficial to human life, either at home or...

  8. Antiulcerogenic activity of Carica papaya seed in rats.

    Science.gov (United States)

    Pinto, Lorraine Aparecida; Cordeiro, Kátia Wolff; Carrasco, Viviane; Carollo, Carlos Alexandre; Cardoso, Cláudia Andréa Lima; Argadoña, Eliana Janet Sanjinez; Freitas, Karine de Cássia

    2015-03-01

    The purpose of the present study was to evaluate the gastroprotective and healing effects of the methanolic extract of the seed of the papaya Carica papaya L. (MECP) in rats. Models of acute gastric ulcer induction by ethanol and indomethacin and of chronic ulcer by acetic acid were used. The gastric juice and mucus parameters were evaluated using the pylorus ligation model, and the involvement of sulfhydryl compounds (GSH) and nitric oxide in the gastroprotective effect was analyzed using the ethanol model. The toxicity was assessed through toxicity tests. No signs of toxicity were observed when the rats received a single dose of 2000 mg/kg of extract. The MECP in doses of 125, 250, and 500 mg/kg significantly reduced the gastric lesion with 56, 76, and 82 % inhibition, respectively, and a dose of 30 mg/kg lansoprazole showed 79 % inhibition in the ethanol model. MECP (125, 250, 500 mg/kg) and cimetidine (200 mg/kg) reduced the gastric lesion in the indomethacin model, with 62, 67, 81, and 85 % inhibition, respectively. The MECP (500 mg/kg) and cimetidine (200 mg/kg) treatments showed a reduction in ulcerative symptoms induced by acetic acid by 84 and 73 %, respectively. The antiulcerogenic activity seems to involve GSH because the inhibition dropped from 72 to 13 % in the presence of a GSH inhibitor. Moreover, the MECP showed systemic action, increasing the mucus production and decreasing gastric acidity. Treatments with MECP induce gastroprotection without signs of toxicity. This effect seems to involve sulfhydryl compounds, increased mucus, and reduced gastric acidity. PMID:25418890

  9. Study about papaya (Carica papaya L.) conservation submitted to a combined treatment of irradiation and an edible film of quitosan

    International Nuclear Information System (INIS)

    In this research, on a first stage the effect of two treatments were studied: thermal, and a chitosan edible coating, combined with four different irradiation doses: 0,00 kGy, 0,50 kGy, 0,75 kGy and 1,00 kGy for effects in the ripening of papaya (Carica Papaya) cv. Golden. To evaluate the influence of these two factors in the physical-chemical characteristics of the fruit during ripening, analysis on color, both skin and pulp, firmness, soluble solids, pH and titrable acidity were performed. The incidence of fungi in fruit skin was also analyzed. The fruits were first maintained at 10 deg C chamber for fifteen days to simulate maritime transportation to the importer country and then transferred to a 25 deg C chamber aiming simulation of the commercialization conditions. From this first step, the conclusion was that chitosan in the concentration of 2% was not able to contain the fungi development and that it also influenced in a significant form the variable pH. A more expressive result was obtained for irradiation doses of 0,75 kGy and 1,00 kGy, as it was observed in the beginning the acceleration in ripening through the variables skin color and penetration energy of the fruit pulp. When storage temperature was changed to 25 deg C this situation inverted for these same variables and a two day delay was observed on the development of yellow color on the fruit skin and loss of pulp firmness. The variables titrable acidity, soluble solids and pulp color were not influenced by treatment or by irradiation. On a second stage, the fruits, in the same condition of the previous stage, were analyzed in relation of their mass and ripening stage using a ripening scale provided by the producer of the fruits for this experiment, as to evaluate the gradual development of fruit ripening according to the various treatments (chitosan 3%, thermal or control) and irradiation dose (0,00 kGy and 0,75 kGy). Confirming the results obtained in the first stage, the irradiation was able to delay ripening in one day and a half approximately. The chitosan in these new conditions of test propitiated fungi development and the fruits coated with chitosan did not ripe in the way it was seen to come. A last stage was done to verify consumer behavior in respect to an irradiated papaya. By three different sensorial tests it was verified if provers were able to perceive significant difference between irradiated and non irradiated samples, what was the acceptance of a irradiated sample and the intention of purchase of a irradiated and a non irradiated papaya. Conclusion from these tests was that the difference established in the triangular test was not enough to establish significant difference between the acceptances of the two samples in the four parameters tested: appearance, texture, aroma and flavor. For the purchase intention it was verified that the irradiation is not a barrier for the consumer. (author)

  10. Use of microsatellite markers in molecular analysis of segregating populations of papaya (Carica papaya L.) derived from backcrossing.

    Science.gov (United States)

    Pinto, F O; Pereira, M G; Luz, L N; Cardozo, D L; Ramos, H C C; Macedo, C M P

    2013-01-01

    Brazil is the world leader in papaya production. However, only a small number of cultivars are registered for commercial planting, mainly owing to delays in obtaining cultivars and the high costs of the field phase of breeding programs. These costs can be reduced when molecular tools are combined with conventional breeding methods. In the present study, we conducted a molecular analysis of a self-fertilized population of a first backcrossing generation of BC1S1 papaya plants via microsatellite markers both to monitor the level of homozygosity and the gene/allele transfer that confers the Golden trait (fruit color) and to assess the parental genomic proportion in the genotypes studied. Based on the analysis of 20 polymorphic microsatellite loci, 19 genotypes with the Golden trait belonging to BC1S1 were evaluated in addition to the parental genotypes. Genetic distance was estimated through weighted index. The genotypes were then grouped using the hierarchical nearest neighbor method, and the analysis of principal coordinates was used to measure the proportion of parental genomes in the segregating genotypes. The mean value of the inbreeding coefficient was 0.36. The analysis of the principal coordinates revealed that on average, 64% of the recurrent parent genome was present in the population. Together, the analyses allowed the selection of 3 individuals for the next backcross cycle (33BC1S1-18, 34BC1S1-16, and 37BC1S1-10). These individuals had a higher proportion of the recurrent parent and were grouped close to the recurrent parent in the cluster analysis. PMID:23884768

  11. Molecular diagnosis of Papaya meleira virus (PMeV) from leaf samples of Carica papaya L. using conventional and real-time RT-PCR.

    Science.gov (United States)

    Abreu, Paolla M V; Piccin, Joo G; Rodrigues, Silas P; Buss, David S; Ventura, Jos A; Fernandes, Patricia M B

    2012-03-01

    Papaya meleira virus (PMeV) is the causal agent of papaya sticky disease. This study describes two methods for molecular diagnosis of PMeV using conventional and real-time PCR. These methods were shown to be more efficient than current methods of viral detection using extraction of PMeV dsRNA and observation of symptoms in the field. The methods described here were used to evaluate the effect of inoculation of papaya plants with purified PMeV dsRNA on the progress of PMeV infection. A single inoculation with PMeV dsRNA was observed to delay the progress of the virus infection by several weeks. The possibility of vertical transmission of PMeV was also investigated. No evidence was found for PMeV transmission through seeds collected from diseased fruit. The implications of these results for the epidemiology of PMeV and the management of papaya sticky disease are discussed. PMID:22193169

  12. Recombinant pro-regions from papain and papaya proteinase IV-are selective high affinity inhibitors of the mature papaya enzymes.

    Science.gov (United States)

    Taylor, M A; Baker, K C; Briggs, G S; Connerton, I F; Cummings, N J; Pratt, K A; Revell, D F; Freedman, R B; Goodenough, P W

    1995-01-01

    Proteolytic enzymes require the presence of their pro-regions for correct folding. Of the four proteolytic enzymes from Carica papaya, papain and papaya proteinase IV (PPIV) have 68% sequence identity. We find that their pro-regions are even more similar, exhibiting 73.6% identity. cDNAs encoding the pro-regions of these two proteinases have been expressed in Escherichia coli independently from their mature enzymes. The recombinant pro-regions of papain and PPIV have been shown to be high affinity inhibitors of all four of the mature native papaya cysteine proteinases. Their inhibition constants are in the range 10(-6) - 10(-9) M. PPIV was inhibited two to three orders of magnitude less effectively than papain, chymopapain and caricain. The pro-region of PPIV, however, inhibited its own mature enzyme more effectively than did the pro-region of papain. Alignment of the sequences of the four papaya enzymes shows that there is a highly variable section towards the C-terminal of the pro-region. This region may therefore confer selectivity to the pro-regions for the individual proteolytic enzymes. PMID:7770454

  13. Low-dose gamma irradiation following hot water immersion of papaya (Carica papaya linn.) fruits provides additional control of postharvest fungal infection to extend shelf life

    International Nuclear Information System (INIS)

    Low-dose gamma irradiation (0.08 kGy over 10 min), a level significantly below that required to satisfy the majority of international quarantine regulations, has been employed to provide a significant reduction in visible fungal infection on papaya fruit surfaces. This is appropriate for local and national markets in producer countries where levels of commercial acceptability can be retained despite surface lesions due to fungal infection. Irradiation alone and in combination with hot-water immersion (50 °C for 10 min) has been applied to papaya (Carica papaya L.) fruits at both the mature green and 1/3 yellow stages of maturity. The incidence and severity of surface fungal infections, including anthracnose, were significantly reduced by the combined treatment compared to irradiation or hot water treatment alone, extending storage at 11 °C by 13 days and retaining commercial acceptability. The combined treatment had no significant, negative impact on ripening, with quality characteristics such as surface and internal colour change, firmness, soluble solids, acidity and vitamin C maintained at acceptable levels. - Highlights: • Storage of papaya extended to 28 days whilst retaining commercial quality. • Additive effect of low gamma irradiation (0.08 kGy over 10 min) and hot-water treatment. • Significant reduction in surface fungal lesions. • No significant impact on colour change or flesh quality during storage

  14. Relationships between SAP-flow measurements, whole-canopy transpiration and reference evapotranspiration in field-grown papaya (Carica papaya L.)

    Science.gov (United States)

    Whole-canopy gas exchange measurement in papaya can provide a scientific basis to optimize irrigation, and fruit yield and quality. The objectives of this study were to: 1) verify the relationship between xylem sap flow measured by the heat coefficient method and whole canopy transpiration in ‘Gra...

  15. Production of Internal Yellowing Symptoms on Resistant and Susceptible Papaya Cultivars by Enterobacter cloacae at Varying Inoculum Concentrations

    Science.gov (United States)

    Internal yellowing (IY) is a bacterial disease of ripening papaya flesh caused by Enterobacter cloacae and characterized by yellow softening tissue. IY restricts food safety of value-added products like fresh or frozen papaya cubes. The incidence of E. cloacae presumably differs in resistant (R) a...

  16. 76 FR 49725 - Notice of Decision To Authorize the Importation of Fresh Papaya Fruit From Malaysia into the...

    Science.gov (United States)

    2011-08-11

    ..., 2011 (76 FR 13972, Docket No. APHIS-2011- 0013), in which we announced the availability, for review and... Importation of Fresh Papaya Fruit From Malaysia into the Continental United States AGENCY: Animal and Plant... authorize the importation into the continental United States of fresh papaya fruit from Malaysia. Based...

  17. Phytochemical screening and hypoglycemic activity of Carica papaya leaf in streptozotocin-induced diabetic rats

    Directory of Open Access Journals (Sweden)

    Isela E. Jurez-Rojop

    2014-06-01

    Full Text Available The extraction of plant constituents is essential to isolate biologically active compounds, aimed to understand their role on the treatment of diabetes. This study was designed to explore the preliminary phytochemical and physicochemical analysis of Carica papaya L., Caricaceae, leaf, and further evaluation of its hypoglycemic effect on diabetic rats. C. papaya leaves were extracted using chloroform, n-hexane or ethanol. For each extract a phytochemical screening was performed. The tests were conducted in triplicate and the qualitative and quantitative determination of the various metabolites was done using analytical standards proposed by Mexican Herbal Pharmacopoeia. The chloroform extract, containing steroids and quinones as major components, was chosen to study C. papaya biological effects. The chloroform extract was evaporated to dryness, and doses 0, 31, 62, 125 mg/kg were orally administered in 300 l polyethylene glycol to diabetic rats; and 0 and 62 mg/kg to non-diabetic rats. After a 20-day treatment with the chloroform extract, the animals were sacrificed and blood was obtained for biochemical studies. The main effect observed was a decrease in serum glucose, triglycerides and transaminases in diabetic rats after the administration of C. papaya chloroform extract. These results confirm the potential beneficial action of C. papaya to treat the symptoms of diabetic patients.

  18. Effectiveness of dried Carica papaya seeds against human intestinal parasitosis: a pilot study.

    Science.gov (United States)

    Okeniyi, John A O; Ogunlesi, Tinuade A; Oyelami, Oyeku A; Adeyemi, Lateef A

    2007-03-01

    The tropical fruit Carica papaya and its seeds have proven antihelminthic and anti-amoebic activities. To determine the effectiveness of air-dried C. papaya seeds on human intestinal parasitosis, 60 asymptomatic Nigerian children with stool microscopic evidence of intestinal parasites received immediate doses (20 mL) of either an elixir composed with air-dried C. papaya seeds and honey (CPH) or honey alone (placebo) in two randomized treatment groups. Repeat stool microscopic examinations were conducted 7 days postintervention for intestinal parasites. Significantly more subjects given CPH elixir than those given honey had their stools cleared of parasites [23 of 30 (76.7%) vs. five of 30 (16.7%); z = 4.40, P = .0000109]. There were no harmful effects. The stool clearance rate for the various types of parasites encountered was between 71.4% and 100% following CPH elixir treatment compared with 0-15.4% with honey. Thus, air-dried C. papaya seeds are efficacious in treating human intestinal parasites and without significant side effects. Their consumption offers a cheap, natural, harmless, readily available monotherapy and preventive strategy against intestinal parasitosis, especially in tropical communities. Further and large-scale intervention studies to compare C. papaya with standard antiparasitic preparation are desirous. PMID:17472487

  19. Eradication of Bactrocera papayae (Diptera: Tephritidae) by male annihilation and protein baiting in Queensland, Australia

    International Nuclear Information System (INIS)

    An established population of Bactrocera papayae Drew and Hancock (Asian papaya fruit fly) was detected on the Australian mainland near Cairns, north Queensland, on 16 October 1995 (Fay et al. 1997). Coincidentally, the first flies were bred from green papaya, a host not normally utilised in the unripe state by indigenous fruit fly species. B. papayae is a member of the Oriental fruit fly (Bactrocera dorsalis) complex (Drew and Hancock 1994) and has more than 190 known hosts in Southeast Asia, where it is a major pest species. Consequently, the threat to Australian horticulture posed by the incursion was recognised immediately. By 26 October 1995, breeding populations were confirmed around Cairns, Mossman and Mareeba and a Pest Quarantine Area (PQA) was legislated. By 1 November 1995, following detections south of Innisfail, the PQA boundaries were set at 144 deg. 15'E, 19 deg. 00'S, covering nearly 78,000 km2. B. papayae was ultimately trapped over some 20,000 km2. A national eradication campaign began in November 1995, undertaken by the Queensland Department of Primary Industries

  20. Planting spacing and NK fertilizing on physiological indexes and fruit production of papaya under semiarid climate

    Directory of Open Access Journals (Sweden)

    Eduardo Monteiro Santos

    2015-01-01

    Full Text Available ABSTRACT The nutritional requirements of papaya (Carica papaya L. increase continuously throughout the crop cycle, especially for potassium and nitrogen, which are the most required nutrients and act on plant vital functions such as photosynthetic activity, respiration, transpiration and stomatal regulation. An experiment was conducted from November 2010 to December 2012 to evaluate physiological indexes and fruit production of papaya cv. Caliman-01 as a function of planting spacing and NK fertilizing. The experimental design consisted of randomized blocks, with treatments distributed in a factorial arrangement (2 4 4, using 2 planting spacing [simple rows (3.8 2.0 m and double rows (3.8 2.0 1.8 m], 4 nitrogen doses (320, 400, 480 and 560 g of N per plant-1 and 4 potassium doses (380, 475, 570 and 665 g of K2O per plant-1 with 4 replications of 3 plants each. The following variables were evaluated: leaf area index (LAI, leaf chlorophyll index (a, b and total index, intercepted photosynthetically active radiation (Int.PAR, in mol?m-2?s-1, efficiency use of photosynthetically active radiation (Ef.PAR and fruit yield. The fruit production and physiological characteristics of papaya cv. Caliman-01 depend on planting spacing. Under the soil, climate and plant conditions of this study, 665 g of K2O and 320 g of N per plant under double spacing could be recommended for the production of papaya cv. Caliman-01.

  1. Phytochemical screening and hypoglycemic activity of Carica papaya leaf in streptozotocin-induced diabetic rats

    Scientific Electronic Library Online (English)

    Isela E., Jurez-Rojop; Carlos, A.Tovilla-Zrate; Dora E., Aguilar-Domnguez; Luis F. Roa-de la, Fuente; Carlos E., Lobato-Garca; Jorge L., Bl-Castillo; Leonor, Lpez-Meraz; Juan C., Daz-Zagoya; Deysi Y., Bermdez-Ocaa.

    2014-06-01

    Full Text Available The extraction of plant constituents is essential to isolate biologically active compounds, aimed to understand their role on the treatment of diabetes. This study was designed to explore the preliminary phytochemical and physicochemical analysis of Carica papaya L., Caricaceae, leaf, and further ev [...] aluation of its hypoglycemic effect on diabetic rats. C. papaya leaves were extracted using chloroform, n-hexane or ethanol. For each extract a phytochemical screening was performed. The tests were conducted in triplicate and the qualitative and quantitative determination of the various metabolites was done using analytical standards proposed by Mexican Herbal Pharmacopoeia. The chloroform extract, containing steroids and quinones as major components, was chosen to study C. papaya biological effects. The chloroform extract was evaporated to dryness, and doses 0, 31, 62, 125 mg/kg were orally administered in 300 l polyethylene glycol to diabetic rats; and 0 and 62 mg/kg to non-diabetic rats. After a 20-day treatment with the chloroform extract, the animals were sacrificed and blood was obtained for biochemical studies. The main effect observed was a decrease in serum glucose, triglycerides and transaminases in diabetic rats after the administration of C. papaya chloroform extract. These results confirm the potential beneficial action of C. papaya to treat the symptoms of diabetic patients.

  2. Carbon disulfide formation in papaya under conditions of dithiocarbamate residue analysis.

    Science.gov (United States)

    Abakerli, R B; Sparrapan, R; Sawaya, A C H F; Eberlin, M N; Jara, J L P; Rodrigues, N R; Fay, E F; Luiz, A J B; Galvão, T D L; Martins, D dos S; Yamanishi, O K; Toledo, H H B

    2015-12-01

    Golden, Sunrise Solo and Tainung cultivars of papaya were found to release CS2 when submitted to experimental conditions of dithiocarbamate residue analysis. Three common analytical methods were used to quantitate CS2; one spectrophotometric method and two chromatographic methods. All three methods gave positive CS2 results for all three papaya varieties. Other endogenous compounds present in isooctane extracts of papaya fractions detected via gas chromatography (GC/ITD) using electron ionization (EI) were: carbonyl sulfide, dimethyl sulfide, carbon disulfide, 2-methylthiophene, 3-methylthiophene, 2-ethylthiophene, 3-ethylthiophene, benzylisothiocyanate, benzylthiocyanate and benzonitrile. Control samples were obtained from papaya plantations cultivated in experimental areas, in which no treatment with fungicides of the dithiocarbamate group was applied. Endogenous CS2 levels were compared with true dithiocarbamate residues measured in papaya samples from the field trials following applications of the mancozeb fungicide. Three days after application, true dithiocarbamate residues, measured by the procedure with isooctane partitioning and GC-ITD, were at the average level of 2 mg kg(-1). PMID:26041166

  3. KURSUS SINGKAT DAN PELATIHAN PEMANFAATAN PEPAYA (CARISA PAPAYA L. MENJADI MANISAN KERING BUAH PEPAYA DI DESA TIMUHUN, KECAMATAN BANJARANGKAN, KABUPATEN KLUNGKUNG

    Directory of Open Access Journals (Sweden)

    I Made Nada

    2012-09-01

    Full Text Available ABSTRACT In Desa Timuhun, eventhough papaya is not primary fruit product, but usually papaya is planted among the other plants and easily to grow. Papaya has good potency to be processed to become food product. Matured papaya fruit is usually fresh served for desk fruit. On the other hand, for papaya which is not mature yet, usually used for vegetable. Papaya has high water content, so it makes papaya fruit easy to experience damage. With papaya processing, papaya which is originally assumed improper to be sold or to be consumed (mechanical damage, diseased, acid, physical damage and will only to be thrown, finally can be used. The making of papaya dry candy is one of effort to preserve and to vary form of papaya serving. It also improves the economic value of papaya. The making of papaya can be conducted in big or small scale. The activity objectives are to introduce and give additional knowledge to society about papaya processing technology, to increase economic added value of papaya and the using of papaya which is improper to be sold or to be consumed becoming valuable food product .Realization of the objectives above are by performing an information sharing and discussion with the society about process of the making dry candy from papaya, training of papaya dry candy making, introduce simple technology of dry candy papaya making. This activity was performed at the head of Desa Timuhun office, on Friday, September 14, 2007 and was attended by 26 persons, including association of woman farmer and the staff in that office. The result of this activity shows that there was positive response of people who attend this activity, because by processing papaya fruit into dry candy, have a lot of advantage compare to sale it freshly. Beside that, this dry candy is healthy snack, without synthetic preservation, enriched with vitamins which is useful for health, also this dry candy is still good up to 1 month and of course it could to improve economic value compared to be sold freshly

  4. Metáforas en Lengua de Señas Chilena Metaphors in Chilean Sign Language

    OpenAIRE

    Carolina Becerra

    2008-01-01

    Este estudio describe las características del lenguaje metafórico de personas sordas chilenas y su impacto en la comprensión lingüística. La relevancia de esta pregunta radica en la escasez de investigaciones realizadas, particularmente a nivel nacional. Se desarrolló un estudio cualitativo en base a análisis de videos de sujetos sordos en habla espontánea. Se confeccionó una lista de metáforas conceptuales y no conceptuales en Lengua de Señas Chilena. Posteriormente se evaluó su comprensión ...

  5. The effect of gamma radiation on the chemical content, texture and shelf life of papaya

    International Nuclear Information System (INIS)

    Commercially matured papaya, Carica papaya var. Solo were gamma irradiated at doses of 0 kGy, 0.75 kGy, 1.0 KGy and 1.5 kGy. The production rate of ethylene, development of ripening colour, pectin substances and ascorbic acid content as well as the weight losses were studied during storage at room temperature. Ethylene production, development of ripening colour and softening were found to be retarded by irradiation. Irradiation also slowed down the reduction rate of alcohol insoluble substances and hydrochloric acid soluble pectin and the increment of water soluble pectin and hexamataphosphate soluble pectin. Doses of 1.0 kGy and 1.5 kGy effectively suppressed the synthesis of vitamin C although no effect on the existing ascorbic acid was detected. The weight losses of papaya were not affected by irradiation treatment

  6. Phenol induced by irradiation does not impair sensory quality of fenugreek and papaya

    International Nuclear Information System (INIS)

    The effect of radiation processing on the sensory quality of fenugreek and papaya exposed to doses in the range of 2.5–10 kGy and 100 Gy–2.5 kGy respectively was investigated. Despite an increase in the content of phenol in the volatile oil of these food products overall sensory quality of the irradiated and control samples was not significantly affected by radiation processing. - Highlights: • Sensory quality of fenugreek and papaya is not significantly affected by radiation processing. • Irradiation did not adversely affect the overall acceptability of the processed samples. • Radiation processing was thus successfully applied to the preservation of fenugreek and papaya

  7. Combined treatment of UV and gamma radiation of papaya for decay control

    International Nuclear Information System (INIS)

    An exploratory study was made to determine if combining u.v. and gamma radiation treatment at selective doses would decrease the fungal decay incidence in papaya (Carica papaya L. var. Solo), and thereby increase the marketable life of the fruit. Also studied was the effect of the combined treatment on the spores of Aschochyta spp., Colletotrichium spp. and Phytophthora spp., which are commonly found on papaya grown in Hawaii. This was to test the postulate that the combined treatment would prevent photoreactivation of the disrupted nuclei in microorganisms and lead to eventual death. Experimental results did not indicate a conclusive trend to effectiveness but suggested a number of problems to be resolved before the treatment can become useful in fungal decay control of fruits. (author)

  8. Combined Treatment of UV and Gamma Radiation of Papaya for Decay Control

    International Nuclear Information System (INIS)

    An exploratory study was made to determine if combining u.v. and gamma radiation treatment at selective doses would decrease the fungal decay incidence in papaya (Carica papaya L. var. Solo), and thereby increase the marketable life of the fruit. Also studied was the effect of the combined treatment on the spores of Aschochyta spp., Colletotrichium spp. and Phytophthora spp., which are commonly found on papaya grown in Hawaii. This was to test the postulate that the combined treatment would prevent photoreactivation of the disrupted nuclei in microorganisms and lead to eventual death. Experimental results did not indicate a conclusive trend to effectiveness but suggested a number of problems to be resolved before the treatment can become useful in fungal decay control of fruits. (author)

  9. Dynamic transposable element accumulation in the nascent sex chromosomes of papaya.

    Science.gov (United States)

    Vanburen, Robert; Ming, Ray

    2013-01-01

    From their inception, Y chromosomes in plants and animals are subjected to the powerful effects of Mller's ratchet, a process spurred by suppression of recombination that results in a rapid accumulation of mutations and repetitive elements. These mutations eventually lead to gene loss and degeneration of the Y chromosome. Y chromosomes in mammals are ancient, whereas most sex chromosomes in plants and many in insects and fish evolved recently. Sex type in papaya is controlled by a pair of nascent sex chromosomes that evolved around 7 million years ago. The papaya X and Y(h) were recently sequenced, providing valuable insight into the early stages of sex chromosome evolution. Here we discuss the fruits of this work with a focus on the repeat accumulation, gene trafficking and promiscuous DNA sequences found in the slowly degenerating Y(h) chromosome of papaya. PMID:23734293

  10. Poetas mapuches en la literatura chilena Mapuche poets in Chilean literature

    Directory of Open Access Journals (Sweden)

    Iván Carrasco M

    2000-01-01

    Full Text Available En este trabajo se estudia el modo en que las tradiciones textuales etnoliterarias de los mapuches y literarias de los españoles, se han imbricado en la literatura chilena a través del tiempo, y los aportes que ha dejado la incorporación de poetas de cultura mapuche en la poesía chilena contemporánea. En particular, se establece que autores como Sebastián Queupul, Pedro Alonzo, Elicura Chihuailaf y Leonel Lienlaf han participado en la conformación de la poesía etnocultural, dentro de la cual han propuesto una visión intercultural que sobrepasa los límites de la etnoliteratura mapuche y la literatura chilena tradicional, y un conjunto de estrategias textuales compartidas con otros poetas (enunciación sincrética, intertextualidad transliteraria y codificación plural, en la cual han creado una variedad propia, el doble registro.This paper studies the way in which mapuche textual ethnoliterary traditions and Spanish literary traditions have mixed in the Chilean literature through time, and the contribution of Mapuche poets to contemporary Chilean poetry, particularly, authors such as Sebastián Queupul, Pedro Alonzo, Elicura Chihuailaf and Leonel Lienlaf, where they have created an intercultural view that goes beyond the limits of the mapuche ethnoliterature and Chilean traditional literature, together with a bunch of text strategies shared with others poets (syncretic enunciation, transliterary intertext and plural codification, where they have created their own variety, the double register.

  11. Canto para una semilla: Luis Advis, Violeta Parra y la modernizacin de la msica popular chilena

    Directory of Open Access Journals (Sweden)

    avier Osorio Fernndez

    2006-06-01

    Full Text Available El objetivo del presente artculo es aportar algunas reflexiones sobre la obra Canto para una semilla del compositor chileno Luis Advis (1972, fundamentalmente a partir de sus dimensiones culturales e histricas. El significado de esta obra es observado en relacin con los debates y cuestionamientos a los que se ve enfrentado el compositor en la sociedad chilena durante las dcadas de 1960 y 1970, los cuales se constituyen como expresin de las transformaciones provocadas por la modernizacin en nuestro pas. Esta experiencia concreta de la modernidad se ve reflejada en el contenido potico de las dcimas de Violeta Parra, que constituyen el soporte dramtico de la obra de Luis Advis, as como tambin en las vinculaciones entre msica culta y msica popular, que darn origen posteriormente a la Nueva Cancin Chilena y, con ello, redefinirn tambin el lugar que la msica popular posee en nuestra sociedadThis article is a study of the piece Canto para una semilla (Song for a Seed by the Chilean composer Luis Advis (1972, primarily in its cultural and historical dimensions. The meaning of the work is sought in its interrelations with the problems and debates within the Chilean society of the 1960s and 1970s, which were related to the transformations brought about by modernization in Chile. This is mirrored in the poetic content of the dcimas written by Violeta Parra, which constitute the dramatic support for Luis Advis's work. This is further related to the connections between art music and popular music which will later originate the Nueva Cancin Chilena (New Chilean Song this redefining the place of popular music in our society

  12. Dinmica del crecimiento de papaya por efecto de la inoculacin micorrzica y fertilizacin con fsforo / Growth dynamics of papaya due to mycorrhizal inoculation and phosphorous fertilization

    Scientific Electronic Library Online (English)

    Evangelina Esmeralda, Quiones-Aguilar; Luis, Lpez-Prez; Gabriel, Rincn-Enrquez.

    2014-08-01

    Full Text Available En Mxico, la papaya es un fruto importante de exportacin; sin embargo, este mercado exige calidad bio, constituyendo un reto en la produccin con el uso de tecnologas bio-ecolgicas y sostenibles. Los hongos micorrzicos arbusculares (HMA) pueden contribuir significativamente en la nutricin vege [...] tal, en especial en la obtencin de fsforo (P). El empleo de los HMA en papaya es alentador, pero falta definir aspectos sobre el manejo agronmico de productos a base de HMA. El objetivo de este estudio fue evaluar el efecto del HMA Glomus sp. Zac-2 y la fertilizacin con P, sobre el crecimiento de papaya var. Cera, durante la etapa de vivero. Para ello, se realiz un experimento trifactorial 2 x 3 x 4 (inoculacin HMA, fuente y dosis de P) en un diseo completamente al azar. Las variables evaluadas fueron altura de planta (AP) y dimetro de tallo (DT), cada 15 das; biomasa seca (BS) y densidad de esporas (DE), al final del experimento (90 das). A partir de la BS de plantas con y sin micorrizar, se calcul el ndice relativo de dependencia micorrzica (IRDM). Las variables AP, DT y BS fueron diferentes (P ? 0.05) entre plantas con y sin HMA, independientemente de la dosis y fuente de P. A los 75 das, las plantas con HMA incrementaron su crecimiento ms de 500 % con respecto a las no inoculadas; el IRDM fue 99 %. Estos resultados sugieren que la inoculacin de HMA en papaya durante la fase de vivero puede beneficiar su crecimiento posterior en campo. Abstract in english Papaya is a major fruit export for Mexico; however, the international market is increasingly demanding bio quality, representing a significant production challenge due to the need for bio-ecological and sustainable technologies. Arbuscular mycorrhizal fungi (AMF) can contribute significantly to plan [...] t nutrition, particularly in phosphorous (P) uptake. The use of AMF in papaya is yielding encouraging results, but there is a need to define certain aspects regarding agronomic management of AMF-based products. The aim of this study was to evaluate the effect of AMF Glomus sp. Zac-2 and P fertilization on the growth of Cera var. papaya during the nursery stage. For this, a 2 x 3 x 4 tri-factorial experiment (AMF inoculation, P source and P dose) was conducted in a completely randomized design. The variables plant height (PH) and stem diameter (SD) were evaluated every 15 days; dry biomass (DB) and spore density (SD) were assessed at the end of the experiment (90 days). From the DB of plants with and without mycorrhizal inoculation, the relative mycorrhizal dependency index (RMDI) was calculated. Data analysis showed significant differences (P ? 0.05) between papaya plants with and without AMF for PH, SD and DB, regardless of the P dose and source. At 75 days, growth increases of more than 500 % were recorded in plants with AMF compared to the non-inoculated plants; the RMDI was 99 %. These results suggest that AMF inoculation in papaya during the nursery phase can benefit further growth in the field.

  13. Stability and efficiency of dye-sensitized solar cells based on papaya-leaf dye.

    Science.gov (United States)

    Suyitno, Suyitno; Saputra, Trisma Jaya; Supriyanto, Agus; Arifin, Zainal

    2015-09-01

    The present article reports on the enhancement of the performance and stability of natural dye-based dye-sensitized solar cells (DSSCs). Natural dyes extracted from papaya leaves (PL) were investigated as sensitizers in TiO2-based DSSCs and evaluated in comparison with N719 dye. The acidity of the papaya-leaf extract dyes was tuned by adding benzoic acid. The TiO2 film-coated fluorine-doped tin oxide glass substrates were prepared using the doctor-blade method, followed by sintering at 450 C. The counter electrode was coated by chemically deposited catalytic platinum. The working electrodes were immersed in N719 dye and papaya dye solutions with concentrations of 8 g/100 mL. The absorbance spectra of the dyes were obtained by ultra-violet-visible spectroscopy. The energy levels of the dyes were measured by the method of cyclic voltammetry. In addition, Fourier transform infrared spectroscopy was used to determine the characteristic functionalities of the dye molecules. The DSSC based on the N719 dye displayed a highest efficiency of 0.87% whereas those based on papaya-leaf dye achieved 0.28% at pH 3.5. The observed improved efficiency of the latter was attributed to the increased current density value. Furthermore, the DSSCs based on papaya-leaf dye with pH 3.5-4 exhibited better stability than those based on N719 dye. However, further studies are required to improve the current density and stability of natural dye-based DSSCs, including the investigation of alternative dye extraction routes, such as isolating the pure chlorophyll from papaya leaves and stabilizing it. PMID:25875031

  14. Habitat fragmentation threatens wild populations of Carica papaya (Caricaceae) in a lowland rainforest.

    Science.gov (United States)

    Chávez-Pesqueira, Mariana; Suárez-Montes, Pilar; Castillo, Guillermo; Núñez-Farfán, Juan

    2014-07-11

    • Premise of the study: Wild populations of domesticated species constitute a genetic reservoir and are fundamental to the evolutionary potential of species. Wild papaya (Carica papaya) is a rare, short-lived, gap-colonizing, dioecious tree that persists in the forest by continuous dispersal. Theoretically, these life-history characteristics render wild papaya highly susceptible to habitat fragmentation, with anticipated negative effects on its gene pool. Further, species dioecy may cause founder effects to generate local biases in sex ratio, decreasing effective population size.• Methods: We contrasted the genetic diversity and structure of C. papaya between wild populations from rainforest fragments and continuous forest at Los Tuxtlas, Mexico. We evaluated recent migration rates among populations as well as landscape resistance to gene flow. Finally, we calculated the sex ratio of the populations in both habitats.• Key results: Populations of wild papaya in rainforest fragments showed lower genetic diversity and higher population differentiation than populations in continuous rainforest. Estimates of recent migration rates showed a higher percentage of migrants moving from the continuous forest to the forest fragments than in the opposite direction. Agricultural land and cattle pasture were found to be the most resistant matrices to gene flow. Finally, biased sex ratios were seen to affect the effective population size in both habitats.• Conclusions: The mating system, rarity, and short life cycle of C. papaya are exacerbating the effects of rainforest fragmentation on its genetic diversity, threatening the persistence of its natural populations in the proposed place of origin as well as its genetic reservoir. PMID:25016010

  15. Stability and efficiency of dye-sensitized solar cells based on papaya-leaf dye

    Science.gov (United States)

    Suyitno, Suyitno; Saputra, Trisma Jaya; Supriyanto, Agus; Arifin, Zainal

    2015-09-01

    The present article reports on the enhancement of the performance and stability of natural dye-based dye-sensitized solar cells (DSSCs). Natural dyes extracted from papaya leaves (PL) were investigated as sensitizers in TiO2-based DSSCs and evaluated in comparison with N719 dye. The acidity of the papaya-leaf extract dyes was tuned by adding benzoic acid. The TiO2 film-coated fluorine-doped tin oxide glass substrates were prepared using the doctor-blade method, followed by sintering at 450 °C. The counter electrode was coated by chemically deposited catalytic platinum. The working electrodes were immersed in N719 dye and papaya dye solutions with concentrations of 8 g/100 mL. The absorbance spectra of the dyes were obtained by ultra-violet-visible spectroscopy. The energy levels of the dyes were measured by the method of cyclic voltammetry. In addition, Fourier transform infrared spectroscopy was used to determine the characteristic functionalities of the dye molecules. The DSSC based on the N719 dye displayed a highest efficiency of 0.87% whereas those based on papaya-leaf dye achieved 0.28% at pH 3.5. The observed improved efficiency of the latter was attributed to the increased current density value. Furthermore, the DSSCs based on papaya-leaf dye with pH 3.5-4 exhibited better stability than those based on N719 dye. However, further studies are required to improve the current density and stability of natural dye-based DSSCs, including the investigation of alternative dye extraction routes, such as isolating the pure chlorophyll from papaya leaves and stabilizing it.

  16. EFEITOS DO CAPITAL INTELECTUAL SOBRE O DESEMPENHO FINANCEIRO EM EMPRESAS BRASILEIRAS E CHILENAS

    OpenAIRE

    Salete Turra; Danielle Paná Vergini; Fellipe André Jacomossi; Nelson Hein

    2015-01-01

    O objetivo da pesquisa foi verificar a influência do Capital Intelectual medido pelo Valor Agregado do Capital Intelectual (VACI) sobre o desempenho financeiro em empresas brasileiras e chilenas no ano de 2013. Realizou-se uma pesquisa descritiva, documental, com abordagem quantitativa por meio da técnica estatística correlação canônica. Os dados foram coletados na base de dados da Tomnson®. Os resultados do estudo demonstraram correlação canônica existente entre as variáveis que compõem o VA...

  17. La iglesia chilena a fines del siglo XIX: un paradigma pastoral

    Directory of Open Access Journals (Sweden)

    Camus Ibacache, Misael

    2002-06-01

    Full Text Available Study of the training and consolidation of the eclesiastic structure Chilean during the XlXth century and the two first decades of the XXth century through the documents of the visits ad liminina, the pastoral visits and the resolutions of the Episcopal Meetings.

    Estudio de la formación y consolidación de la estructura eclesiástica chilena durante el siglo XIX y las dos primeras décadas del XX a través de los documentos de las visitas ad liminina, las visitas pastorales y las Actas de las Sesiones Episcopales.

  18. Referentes internacionales para el giro reformista de la izquierda chilena (1975-1990

    Directory of Open Access Journals (Sweden)

    Marcelo Mella Polanco

    2011-01-01

    Full Text Available El presente artículo analiza el giro reformista de la izquierda chilena durante el régimen autoritario de Pinochet. Para lograrlo, se expone cómo la penetración de ideas provenientes del marxismo analítico homogeneizó un importante sector de intelectuales y expertos opositores de Pinochet, constituyendo, de esta forma, un nuevo mainstream político-académico capaz de renovar los marcos epistémicos de las ciencias sociales y diseñar la transición a la democracia.

  19. Verbo de concordancia en la lengua de señas chilena

    OpenAIRE

    Dora Adamo Quintela; Irene Cabrera Ramírez; Pamela Lattapiat Navarro; Ximena Acuña Robertson

    1999-01-01

    El presente trabajo trata sobre una clase verbo de la lengua de señas chilena, el verbo de concordancia. A través de su descripción se da cuenta de los diversos mecanismos utilizados por el sistema de la lengua de señas para establecer la concordancia entre el sujeto y el objeto, que funcionan en la oración mediante el proceso de inflexión gramatical. Una característica relevante de la LSCh consiste en la utilización del espacio para producir modificaciones gramaticales regulares, entendidas ...

  20. Papaya extract to treat dengue: a novel therapeutic option?

    Science.gov (United States)

    Sarala, N; Paknikar, Ss

    2014-05-01

    Dengue is a viral disease that today affects a vast number of people in over 125 countries and is responsible for a sizable number of deaths. In the absence of an effective antiviral drug to treat the disease, various treatments are being investigated. Studies have indicated that the juice of the leaves of the Carica papaya plant from the family Caricaceae could help to increase the platelet levels in these patients. This review describes some of the published studies on this topic. The search was done independently by the two authors using PubMed, Google and the library database and included relevant articles of the last 10 years. A total of 7 studies were included in this review, which were one animal study, one case report, three case series and two randomized controlled trials. Although many of the studies and case reports published in literature lack adequate information, some of the studies do raise the possibility that this treatment could be an important option in the future. Further large-scale studies could establish the usefulness or ineffectiveness of this natural product in the treatment of dengue. PMID:24971201

  1. Potentiating Cancer Immunotherapy Using Papaya Mosaic Virus-Derived Nanoparticles.

    Science.gov (United States)

    Lebel, Marie-Ève; Chartrand, Karine; Tarrab, Esther; Savard, Pierre; Leclerc, Denis; Lamarre, Alain

    2016-03-01

    The recent development of novel immunotherapies is revolutionizing cancer treatment. These include, for example, immune checkpoint blockade, immunomodulation, or therapeutic vaccination. Although effective on their own, combining multiple approaches will most likely be required in order to achieve the maximal therapeutic benefit. In this regard, the papaya mosaic virus nanoparticle (PapMV) has shown tremendous potential as (i) an immunostimulatory molecule, (ii) an adjuvant, and (iii) a vaccine platform through its intrinsic capacity to activate the innate immune response in an IFN-α-dependent manner. Here, we demonstrate that intratumor administration of PapMV significantly slows down melanoma progression and prolongs survival. This correlates with enhanced chemokine and pro-inflammatory-cytokine production in the tumor and increased immune-cell infiltration. Proportions of total and tumor-specific CD8(+) T cells dramatically increase following PapMV treatment whereas those of myeloid-derived suppressor cells (MDSC) concomitantly decrease. Moreover, systemic PapMV administration prevents metastatic tumor-implantation in the lungs. Importantly, PapMV also synergistically improves the therapeutic benefit of dendritic cell (DC)-based vaccination and PD-1 blockade by potentiating antitumor immune responses. This study illustrates the immunostimulatory potential of a plant virus-derived nanoparticle for cancer therapy either alone or in conjunction with other promising immunotherapies in clinical development. PMID:26891174

  2. Color index and correlation with physical and chemical parameters of guava, mango and papaya

    OpenAIRE

    Jedman Dantas Motta; Alexandre Jos de Melo Queiroz; Rossana Maria Feitosa de Figueirdo

    2015-01-01

    It was used a low cost colorimeter determine the color in agricultural products. Color analysis on peel and pulps of guava, papaya and mango were made. The values found in the L*a*b* color spaces were used to calculate color indexes that were related to the soluble solids content, pH and the fruit consistence during the maturation. The results obtained permit to conclude that it is possible to estimate the ripening of papaya over the color peel, having found an accurate relation between peel ...

  3. Molecular characterization and infectivity of Papaya leaf curl China virus infecting tomato in China*

    OpenAIRE

    ZHANG Hui; Ma, Xin-ying; Qian, Ya-juan; Zhou, Xue-Ping

    2010-01-01

    Papaya leaf curl China virus (PaLCuCNV) was previously reported as a distinct begomovirus infecting papaya in southern China. Based on molecular diagnostic survey, 13 PaLCuCNV isolates were obtained from tomato plants showing leaf curl symptoms in Henan and Guangxi Provinces of China. Complete nucleotide sequences of 5 representative isolates (AJ558116, AJ558117, AJ704604, FN256260, and FN297834) were determined to be 2738–2751 nucleotides, which share 91.7%–97.9% sequence identities with PaL...

  4. CHARACTERIZATION OF PAPAYA FRUIT MEDIATED SILVER NANOPARTICLES AND EVALUATION OF ITS ANTIMICROBIAL AND WOUND HEALING ACTIVITY

    Directory of Open Access Journals (Sweden)

    EnamalaNarmadha

    2013-07-01

    Full Text Available The field of Nanotechnology is the most active area of research in modern material science. Though there are many chemical as well as physical methods, green synthesis of nanomaterial is the most emerging method of synthesis. We report the synthesis of antibacterial silver nanoparticles using Papaya. The synthesized AgNPs have been characterized by UV-Vis spectroscopy and SEM microscopy. The silver nanoparticles stabilized by Papaya fruit extract were found to have enhanced antimicrobial activity against well-known pathogenic strains Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus, Proteus vulgaris and E. coli.

  5. Chemical characterization of the flour of peel and seed from two papaya cultivars

    OpenAIRE

    Cludia Mendes dos Santos; Celeste Maria Patto Abreu; Juliana Mesquita Freire; Estela de Rezende Queiroz; Marcelle Mendes Mendona

    2014-01-01

    Papaya is among the currently most important tropical fruits grown in Brazil and in the world. The fruit is mainly consumed fresh although it offers many industrial products. The processing of this fruit, as well as its fresh consumption, results in large amounts of waste, such as peels and seeds. Papaya consumption is one of the causes of significant loss of food value; therefore, new aspects on the use of its waste as by-products, or in the production of food additives, or even the incorpor...

  6. Genetic Diversity Based on Coat Protein of Papaya ringspot virus (Pathotype P) Isolates from Bangladesh

    OpenAIRE

    M. S. Akhter; Basavaraj, Y. B.; A. M. Akanda; Mandal, B; Jain, R. K.

    2013-01-01

    The coat protein (CP) sequences of twelve Papaya ringspot virus (PRSV) (pathotype-P) isolates from six major papaya growing areas were determined and compared with those of published PRSV. The CP coding region varied in size from 846852 nucleotides, encoding a protein of 282284 amino acids. Comparative CP sequence analysis revealed that the PRSV-P isolates originating from Bangladesh were divergent up to 14% at amino acids level. Further, the isolates from Bangladesh shared 8695% amino a...

  7. Effect of ionizing radiation on the activity of pectinesterase in papaya (cultivar solo)

    International Nuclear Information System (INIS)

    Papaya fruits (Carica papaya L.) were exposed to ionizing radiation gamma type ( sup(60)Co), using a dose of 0.7kGy, and then stored in a cold room at 10C with a relative humidity of 85% for a period of 25 days. The pectinesterase activity of the irradiated fruits was found to be similar to that of the non-irradiated fruits during the storage period. The radiation dose maintained the texture and enzyme activity of the irradiated fruits the same as that of the non-irradiated fruits. (author)

  8. Effect of 60Co γ irradiation and GA3 treatment on mutation of Carica papaya L

    International Nuclear Information System (INIS)

    The seeds of Carica papaya L. were irradiated by 60Co γ-rays of 10-40 Gy and treated by GA3 of 10-50 mg/L after irradiation. The results showed that small nuclear cell percentage, chromosome variation percentage and leaf variability of papaya seedling increased with increase of irradiation dose, the pollen fertility and fruit quality decreased. GA3 of 10-50 mg/L treatment after irradiation could alleviate the irradiation harm, and the effect of lower concentration of GA3 was better than higher concentration with lower dose irradiation, however, the effect of higher concentration of GA3 was better with higher dose irradiation

  9. Low-dose gamma irradiation following hot Water immersion of Papaya (Carica Papaya linn.) fruits provides additional control of postharvest fungal infection to extend shelf life

    DEFF Research Database (Denmark)

    Rashid, M.H.A.; Grout, Brian William Wilson; Continella, A.; Mahmud, T.M.M.

    2015-01-01

    Low-dose gamma irradiation (0.08 kGy over 10 min), a level significantly below that required to satisfy the majority of international quarantine regulations, has been employed to provide a significant reduction in visible fungal infection on papaya fruit surfaces. This is appropriate for local and...... national markets in producer countries where levels of commercial acceptability can be retained despite surface lesions due to fungal infection. Irradiation alone and in combination with hot-water immersion (50 °C for 10 min) has been applied to papaya (Carica papaya L.) fruits at both the mature green and...

  10. New records of Discomycetes (Pezizales for the Chilean mycobiota Nuevos registros de Discomycetes (Pezizales para la micobiota chilena

    Directory of Open Access Journals (Sweden)

    PABLO SANDOVAL

    2012-01-01

    Full Text Available Se adicionan a la micobiota chilena tres especies de Pezizales, Byssonectria terrestris (Alb. & Schwein. Pfister, Pseudoplectania nigrella (Pers. Fuckel y Pseudoplectania sphagnophila (Pers. Kreisel, Se describen e ilustran los caracteres diagnsticos y se realizan comentarios acerca de las especies.

  11. Patrones de crecimiento urbano en la Patagonia chilena: el caso de la ciudad de Coyhaique

    Directory of Open Access Journals (Sweden)

    Gerardo Azócar García

    2010-01-01

    Full Text Available Las políticas de Estado junto con la importante oferta/demanda de productos inmobiliarios para población de mayor ingreso han orientado el desarrollo reciente de varias ciudades chilenas, originando nuevas formas urbanas. Si bien estos procesos han sido descritos y analizados para varias ciudades y áreas metropolitanas de Chile central, no existen suficientes antecedentes empíricos que den cuenta de la realidad del desarrollo urbano de las ciudades más australes del país, como el caso de Coyhaique en la Patagonia chilena. Este trabajo pretende identificar y analizar los patrones de urbanización de esta ciudad, en un contexto territorial caracterizado, entre otros aspectos, por la fragmentación geográfica, el aislamiento, dispersión de los centros poblados y concentración de la población en la capital regional. Se analiza la evolución y factores explicativos que dan cuenta de su rápido proceso de urbanización y, sobre la base de documentos técnicos, históricos y geográficos, se presentan estimaciones futuras del crecimiento de la ciudad.

  12. Estudios en papaya mínimamente procesada por deshidratación osmótica

    OpenAIRE

    CEBALLOS CHAN, GERMÁN ENRIQUE

    2008-01-01

    Se ha estudiado el efecto de tratamientos osmóticos con disoluciones de sacarosa de diferente concentración (45 y 65 ºBrix), con y sin aplicación de pulso de vacío y con y sin adición de lactato de calcio (1%), en diferentes propiedades relacionadas con la calidad de papaya, en aras a evaluar la factibilidad de utilizar estos tratamientos en la obtención de papaya mínimamente procesada. Se ha caracterizado la cinética del proceso en láminas de papaya y se ha analizado el efecto de los tratami...

  13. Caracterización de accesiones de papaya (Carica papaya L. a través de marcadores AFLP en Cuba

    Directory of Open Access Journals (Sweden)

    Maruchi Alonso Esquivel

    2010-01-01

    Full Text Available Los marcadores moleculares son herramientas valiosas en los estudios genéticos en plantas, y están siendo empleados exitosamente en programas de mejoramiento principalmente en la elección de progenitores y en la selección. El polimorfismo observado mediante la técnica molecular AFLP (Amplified Fragment Length Polymorphism ha sido de utilidad para estudios de diversidad genética en frutales. En el presente trabajo se realizó la caracterización molecular de 12 accesiones de papaya (Carica papaya L. del banco de germoplasma del Instituto de Investigaciones en Fruticultura Tropical (IIFT, empleando la técnica AFLP. Se evaluaron seis combinaciones de iniciadores para la amplificación selectiva, las cuales amplificaron un total de 431 bandas con 73,3% de polimorfismo. El número total de patrones de bandas identificados fue igual en todas las combinaciones utilizadas, con un porcentaje de identificación alto, lo que sugiere que dichas combinaciones pudieran ser empleadas para estudios de variabilidad genética en papaya. En general, los resultados presentados demuestran que existe diversidad genética entre las accesiones evaluadas, lo cual constituye un reflejo del origen que presentan los genotipos analizados a partir de la introducción de materiales foráneos y la polinización abierta de un grupo de materiales selectos. Por tanto, se recomienda retomar la prospección y selección de accesiones locales, así como la introducción de nuevos genotipos foráneos, como dos vías fundamentales para aumentar la diversidad genética presente en el banco de germoplasma de papaya de Cuba. Palabras clave: marcadores moleculares, polimorfismo, diversidad genética.

  14. Low-dose gamma irradiation following hot water immersion of papaya (Carica papaya linn.) fruits provides additional control of postharvest fungal infection to extend shelf life

    Science.gov (United States)

    Rashid, M. H. A.; Grout, B. W. W.; Continella, A.; Mahmud, T. M. M.

    2015-05-01

    Low-dose gamma irradiation (0.08 kGy over 10 min), a level significantly below that required to satisfy the majority of international quarantine regulations, has been employed to provide a significant reduction in visible fungal infection on papaya fruit surfaces. This is appropriate for local and national markets in producer countries where levels of commercial acceptability can be retained despite surface lesions due to fungal infection. Irradiation alone and in combination with hot-water immersion (50 °C for 10 min) has been applied to papaya (Carica papaya L.) fruits at both the mature green and 1/3 yellow stages of maturity. The incidence and severity of surface fungal infections, including anthracnose, were significantly reduced by the combined treatment compared to irradiation or hot water treatment alone, extending storage at 11 °C by 13 days and retaining commercial acceptability. The combined treatment had no significant, negative impact on ripening, with quality characteristics such as surface and internal colour change, firmness, soluble solids, acidity and vitamin C maintained at acceptable levels.

  15. Potencial antifúngico de extractos de cuatro especies vegetales sobre el crecimiento de Colletotrichum gloeosporioides en papaya (Carica papaya en poscosecha

    Directory of Open Access Journals (Sweden)

    Nadia Landero Valenzuela

    2013-01-01

    Full Text Available El manejo de antracnosis por Colletotrichum gloeosporioides es el problema más importante en poscosecha de frutos tropicales. La actividad antifúngica de diferentes extractos vegetales fue evaluada in vitro e in vivo para controlar la antracnosis poscosecha en papaya. Extractos de ajo (Allium sativum (10 y 15 % y canela (Cinnamomum zeylanicum (0,0050; 0,0100; 0,0150 % mostraron efecto fungicida en contra de C. gloeosporioides para supresión de crecimiento micelial (100 %, inhibición de germinación (100 % y esporulación del hongo (100 %, considerándose estos extractos como los más promisorios para inhibir el desarrollo del hongo in vitro. Estudios in vivo también revelaron que los extracto de ajo (11,74 % y canela a dosis de 0,0054 % aplicada antes y al mismo tiempo de la inoculación con C. gloeosporioides, fueron las dosis óptimas para el control (severidad de la antracnosis en frutos de papaya artificialmente inoculados, mientras que a mayores dosis la severidad aumentó. La variable fitotoxicidad arrojó resultados consistentes con lo anterior, comprobándose que al aumentar las dosis de extracto de canela se presentó cambio de color en los frutos. Los resultados sugieren la posibilidad de usar estos extractos a dosis adecuadas como biofungicidas para controlar antracnosis en papaya en poscosecha.

  16. Estudio de la conservación de la papaya (Carica papaya L. asociado a la aplicación de películas comestibles

    Directory of Open Access Journals (Sweden)

    Alessandra Almeida Castro

    2011-06-01

    Full Text Available Brasil es un importante productor de papaya, pero sólo una pequeña parte se exporta. Esta fruta es altamente perecedera, por lo que el control de la maduración es esencial para aumentar la vida útil, tanto para el mercado interno como para la exportación. Una alternativa que ha cobrado impulso en el mercado es el uso de películas comestibles biodegradables, entre ellas la película producida a partir de almidón de yuca. En este estudio se evaluó el efecto de una película de almidón de yuca (2 % sobre la papaya almacenadas a temperatura ambiental (25 ± 2 ºC y a 8 °C, y 82 % de humedad relativa durante 6 días de almacenamiento. Se realizaron análisis de pérdida de peso, color, pH, acidez, carotenoides totales, vitamina C y sólidos solubles totales, cada tres días de almacenamiento. La película comestible de almidón de yuca resultó ser una buena alternativa para la preservación de la papaya durante seis días de almacenamiento, en relación a los parámetros pH, grados Brix, acidez y carotenoides.

  17. Estudio de la conservación de la papaya (Carica papaya L. asociado a la aplicación de películas comestibles

    Directory of Open Access Journals (Sweden)

    Alessandra Almeida Castro

    2011-01-01

    Full Text Available Brasil es un importante productor de papaya, pero sólo una pequeña parte se exporta. Esta fruta es altamente perecedera, por lo que el control de la maduración es esencial para aumentar la vida útil, tanto para el mercado interno como para la exportación. Una alternativa que ha cobrado impulso en el mercado es el uso de películas comestibles biodegradables, entre ellas la película producida a partir de almidón de yuca. En este estudio se evaluó el efecto de una película de almidón de yuca (2 % sobre la papaya almacenadas a temperatura ambiental (25 ± 2 ºC y a 8 °C, y 82 % de humedad relativa durante 6 días de almacenamiento. Se realizaron análisis de pérdida de peso, color, pH, acidez, carotenoides totales, vitamina C y sólidos solubles totales, cada tres días de almacenamiento. La película comestible de almidón de yuca resultó ser una buena alternativa para la preservación de la papaya durante seis días de almacenamiento, en relación a los parámetros pH, grados Brix, acidez y carotenoides.

  18. Preservation of papaya by gamma radiation of cobalt 60

    International Nuclear Information System (INIS)

    The preservation of papaya cultivar Solo was submit to the irradiation at a dose of 80 krad and storage in room temperature to 250 C and with refrigeration to 100 C and the mean relative humidity of the air was 85% and 92% respectively, was studied. First the fruits was submit to the fungi control, with hot water, 470 C to 20 min. After the packaging in carton box the fruits was immediately irradiated by EMBRARAD S.A. and after that storage. Fruits were storage at room temperature was ripened after 9 days. Any difference was observed inside and outside colour of the irradiated and the control fruits. The only difference was the texture. In the radiated ripped fruit was 1,95 kg/cm2 and by the control samples 0,80 kg/cm2. Respiration of irradiated fruit was more active in comparison with the control samples, but don't enter into senescence. Storage at 100 C was the best results being obtained for 30 days such the control sample with the irradiated. But the irradiated fruit was more yellow and uniform in relation to the control samples. Also the texture was more consistent in mean of 2,12 kg/cm2 and 1,36 kg/cm2, respectively. In that temperature the respiration of irradiated fruits was minor in comparison with the control samples. During the storage don't have difference in the weight losses, between the irradiated and the control samples fruits. (author)

  19. Purification of papain from fresh latex of Carica papaya

    Directory of Open Access Journals (Sweden)

    Rubens Monti

    2000-01-01

    Full Text Available In the present study we wish to report a method of crystallizing papain from fresh papaya latex which gave higher yields than previously reported. This method does not involve the use of sulphydryl reagents. The papain thus obtained is practically pure and shows a single band when submitted to electrophoresis on polyacrylamide gel, and is identical to the papain obtained by other methods. In routine enzymatic assays, specific activity was measured using Z-gly-pNP and BAEE as substrates. Papain crystallized by this method, without the use of high concentrations of salts or thiol-containing substances such as cysteine and dithiothreitol, is obtained in the form of a complex with natural inhibitors existent in latex which can be removed by dialysis.No presente trabalho apresenta-se um método de cristalização da papaína oriunda do látex fresco de mamão, o qual apresenta uma alta produtividade em relação aos métodos previamente descritos. A metodologia aqui descrita não envolve o uso de reagentes sulfidrílicos, a papaína foi obtida de forma praticamente pura, apresentando uma simples banda quando submetida a eletroforese, e com propriedades idênticas àquelas obtidas por outros métodos. A atividade específica foi determinada utilizando Z-gly-pNP e BAEE como substrato. A papaína obtida por essa metodologia, sem uso de substâncias tais como cisteína e ditiotreitol, apresenta-se na forma de um complexo com inibidores naturais, os quais podem ser removidos por diálise.

  20. Thrombocyte counts in mice after the administration of papaya leaf suspension.

    Science.gov (United States)

    Sathasivam, Kathiresan; Ramanathan, Surash; Mansor, Sharif M; Haris, Mas Rosemal M H; Wernsdorfer, Walther H

    2009-10-01

    Following up a popular use of crude leaf preparations from Carica papaya for the treatment of dengue infections, a suspension of powdered Carica papaya leaves in palm oil has been investigated for its effect on thrombocyte counts in mice, administering by gavage 15 mg of powdered leaves per kg body weight to 5 mice. Equal numbers of animals received corresponding volumes of either palm oil alone or physiological saline solution. Thrombocyte counts before and at 1, 2, 4, 8, 10, 12, 24, 48 and 72 hours after dosing revealed significantly higher mean counts at 1, 2, 4, 8, 10 and 12 after dosing with the C. papaya leaf formulation as compared to the mean count at hour 0. There was only a non-significant rise of thrombocyte counts in the group having received saline solution, possibly the expression of a normal circadian rhythm in mice. The group having received palm oil only showed a protracted increase of platelet counts that was significant at hours 8 and 48 and obviously the result of a hitherto unknown stimulation of thrombocyte release. The results call for a dose-response investigation and for extending the studies to the isolation and identification of the C. papaya substances responsible for the release and/or production of thrombocytes. PMID:19915811

  1. Chemical Characterization and in Vitro Cytotoxicity on Squamous Cell Carcinoma Cells of Carica Papaya Leaf Extracts

    Science.gov (United States)

    Nguyen, Thao T.; Parat, Marie-Odile; Hodson, Mark P.; Pan, Jenny; Shaw, Paul N.; Hewavitharana, Amitha K.

    2015-01-01

    In traditional medicine, Carica papaya leaf has been used for a wide range of therapeutic applications including skin diseases and cancer. In this study, we investigated the in vitro cytotoxicity of aqueous and ethanolic extracts of Carica papaya leaves on the human oral squamous cell carcinoma SCC25 cell line in parallel with non-cancerous human keratinocyte HaCaT cells. Two out of four extracts showed a significantly selective effect towards the cancer cells and were found to contain high levels of phenolic and flavonoid compounds. The chromatographic and mass spectrometric profiles of the extracts obtained with Ultra High Performance Liquid Chromatography-Quadrupole Time of Flight-Mass Spectrometry were used to tentatively identify the bioactive compounds using comparative analysis. The principal compounds identified were flavonoids or flavonoid glycosides, particularly compounds from the kaempferol and quercetin families, of which several have previously been reported to possess anticancer activities. These results confirm that papaya leaf is a potential source of anticancer compounds and warrant further scientific investigation to validate the traditional use of papaya leaf to treat cancer. PMID:26712788

  2. Changes in protein profile during coagulation of latex from Carica papaya

    OpenAIRE

    L.G. Silva; O. Garcia; M.T.P. Lopes; C. E. Salas

    1997-01-01

    We describe the changes in peptide composition by SDS-PAGE analysis of latex from Carica papaya collected at various times after incision of the unripe fruit. The data show that during latex coagulation several peptides are processed in an orderly fashion.

  3. Anthelmintic activity of papaya seeds on Hymenolepis diminuta infections in rats.

    Science.gov (United States)

    Sapaat, A; Satrija, F; Mahsol, H H; Ahmad, A H

    2012-12-01

    The purpose of this study is to see the anthelmintic activity potential of papaya seeds against Hymenolepis diminuta in rats. The objectives of this study were: (1) to determine the effectiveness of papaya seeds on helminths especially H. diminuta in rats and (2) to determine the effective dose level on helminths in rats. Thirty six male rats from strain Sprague-Dawley were chosen as samples in this experiment. Two types of dose level were used for papaya seeds treatments such as 0.6 g kg-1 and 1.2 g kg-1. The geometric mean (GEM) was used to calculate mean for eggs per gram (EPG) before and after the treatment to be included in the reduction percentage calculation. After 21 days post treatment, necropsies were done to get the worm count and the GEM was used to calculate the efficacy percentage for the treatment. Results from this study showed that the reduction percentages in EPG for papaya seeds treatment for both doses level were very high which is 96.8% for 0.6g kg-1 dose level and 96.2% for 1.2 g kg-1 dose level. Whereas the efficacy percentage based on the worm counts for both doses level were also very high that was 90.77% for 0.6 g kg-1 dose level and 93.85% for 1.2 g kg-1. PMID:23202594

  4. Autosomal Degeneration as the Process for the Evolution of a Primitive Y Chromosome in Papaya

    Science.gov (United States)

    Plant sex chromosomes, in contrast to those in animals, evolved recently and only a few are heteromorphic. Genomic analyses of the homomorphic sex chromosomes of papaya show features of the early stages of sex chromosome evolution. Sequence analysis of 20% of the male specific region (MSY) shows tha...

  5. Chemical characterization of the flour of peel and seed from two papaya cultivars

    Directory of Open Access Journals (Sweden)

    Cláudia Mendes dos Santos

    2014-06-01

    Full Text Available Papaya is among the currently most important tropical fruits grown in Brazil and in the world. The fruit is mainly consumed fresh although it offers many industrial products. The processing of this fruit, as well as its fresh consumption, results in large amounts of waste, such as peels and seeds. Papaya consumption is one of the causes of significant loss of food value; therefore, new aspects on the use of its waste as by-products, or in the production of food additives, or even the incorporation of its flour in food have aroused great interest because these are products of high nutritional value, and their use may be economically viable. The objective of this study was to produce and characterize peel and seed flours from two papaya cultivars (Havai and Calimosa for their chemical constituents focusing on possible use in foods. The proximate and mineral composition, titratable acidity, soluble solids, pH, contents of vitamin C, and phenolic compounds were determined. According to the results obtained, the papaya peel and seed flours had high contents of protein and fiber and therefore can be used as alternative sources of nutrients and can also be added in foods avoiding waste and adding value to the fruit.

  6. Chromosomal location and gene paucity of the male specific region on papaya Y chromosome

    Czech Academy of Sciences Publication Activity Database

    Yu, Q.; Hou, S.; Hobza, Roman; Feltus, F.A.; Wang, X.; Jin, W.; Skelton, R.L.; Blas, A.; Lemke, C.; Saw, J.H.; Moore, P.H.; Alam, M.; Jiang, J.; Paterson, A.H.; Vyskot, Boris; Ming, R.

    2007-01-01

    Roč. 278, č. 2 (2007), s. 177-185. ISSN 1617-4615 R&D Projects: GA ČR(CZ) GA521/06/0056 Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702 Keywords : Carica papaya * repetitive sequences * sex chromosome Subject RIV: BO - Biophysics Impact factor: 2.978, year: 2007

  7. Application of Transgenic Technologies to Papaya: Developments and Biosafety Assessments in Thailand

    Czech Academy of Sciences Publication Activity Database

    Kertbundit, Sunee; Juříček, Miloslav

    2010-01-01

    Roč. 4, č. 1 (2010), s. 52-57. ISSN 1749-0413 Institutional research plan: CEZ:AV0Z50380511 Keywords : coat protein-mediated resistance * GMO * Papaya ringspot virus Subject RIV: EF - Botanics http://home.ueb.cas.cz/publikace/2010_Kertbundit_TransgenicPlantJournal_52.pdf

  8. Crystallization and preliminary X-ray diffraction studies of the glutaminyl cyclase from Carica papaya latex

    International Nuclear Information System (INIS)

    The glutaminyl cyclase isolated from C. papaya latex has been crystallized using the hanging-drop method. Diffraction data have been collected at ESRF beamline BM14 and processed to 1.7 Å resolution. In living systems, the intramolecular cyclization of N-terminal glutamine residues is accomplished by glutaminyl cyclase enzymes (EC 2.3.2.5). While in mammals these enzymes are involved in the synthesis of hormonal and neurotransmitter peptides, the physiological role played by the corresponding plant enzymes still remains to be unravelled. Papaya glutaminyl cyclase (PQC), a 33 kDa enzyme found in the latex of the tropical tree Carica papaya, displays an exceptional resistance to chemical and thermal denaturation as well as to proteolysis. In order to elucidate its enzymatic mechanism and to gain insights into the structural determinants underlying its remarkable stability, PQC was isolated from papaya latex, purified and crystallized by the hanging-drop vapour-diffusion method. The crystals belong to the orthorhombic space group P212121, with unit-cell parameters a = 62.82, b = 81.23, c = 108.17 Å and two molecules per asymmetric unit. Diffraction data have been collected at ESRF beamline BM14 and processed to a resolution of 1.7 Å

  9. Molecular and Clinical Effects of Green Tea and Fermented Papaya Preparation on Diabetes and Cardiovascular Diseases

    Science.gov (United States)

    2014-11-14

    Assess the Effect of Green Tea on Diabetes; Assess the Effect of Fermented Papaya Pretration on Diabetes; Effects of Green Tea and FPP on C-reactive Proteins; Effects of Green Tea and FPP of Lipid Profiles in Diabetes; Effect of Green Tea and FPP on Atheroma Formation

  10. Crystallization and preliminary X-ray diffraction studies of the glutaminyl cyclase from Carica papaya latex

    Energy Technology Data Exchange (ETDEWEB)

    Azarkan, Mohamed [Laboratoire de Chimie Générale I, Faculté de Médecine-ULB CP609, 808 Route de Lennik, B-1070 Brussels (Belgium); Clantin, Bernard; Bompard, Coralie [CNRS-UMR 8525, Institut de Biologie de Lille, BP 477, 1 Rue du Professeur Calmette, F-59021 Lille (France); Belrhali, Hassan [EMBL Grenoble Outstation, 6 Rue Jules Horowitz, BP 181, F-38042 Grenoble CEDEX 9 (France); Baeyens-Volant, Danielle [Laboratoire de Chimie Générale I, Faculté de Médecine-ULB CP609, 808 Route de Lennik, B-1070 Brussels (Belgium); Looze, Yvan [Laboratoire de Chimie Générale, Institut de Pharmacie-ULB CP206/04, Boulevard du Triomphe, B-1050 Brussels (Belgium); Villeret, Vincent, E-mail: vincent.villeret@ibl.fr [CNRS-UMR 8525, Institut de Biologie de Lille, BP 477, 1 Rue du Professeur Calmette, F-59021 Lille (France); Wintjens, René, E-mail: vincent.villeret@ibl.fr [Laboratoire de Chimie Générale, Institut de Pharmacie-ULB CP206/04, Boulevard du Triomphe, B-1050 Brussels (Belgium); Laboratoire de Chimie Générale I, Faculté de Médecine-ULB CP609, 808 Route de Lennik, B-1070 Brussels (Belgium)

    2005-01-01

    The glutaminyl cyclase isolated from C. papaya latex has been crystallized using the hanging-drop method. Diffraction data have been collected at ESRF beamline BM14 and processed to 1.7 Å resolution. In living systems, the intramolecular cyclization of N-terminal glutamine residues is accomplished by glutaminyl cyclase enzymes (EC 2.3.2.5). While in mammals these enzymes are involved in the synthesis of hormonal and neurotransmitter peptides, the physiological role played by the corresponding plant enzymes still remains to be unravelled. Papaya glutaminyl cyclase (PQC), a 33 kDa enzyme found in the latex of the tropical tree Carica papaya, displays an exceptional resistance to chemical and thermal denaturation as well as to proteolysis. In order to elucidate its enzymatic mechanism and to gain insights into the structural determinants underlying its remarkable stability, PQC was isolated from papaya latex, purified and crystallized by the hanging-drop vapour-diffusion method. The crystals belong to the orthorhombic space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 62.82, b = 81.23, c = 108.17 Å and two molecules per asymmetric unit. Diffraction data have been collected at ESRF beamline BM14 and processed to a resolution of 1.7 Å.

  11. Chemical Characterization and in Vitro Cytotoxicity on Squamous Cell Carcinoma Cells of Carica Papaya Leaf Extracts

    Directory of Open Access Journals (Sweden)

    Thao T. Nguyen

    2015-12-01

    Full Text Available In traditional medicine, Carica papaya leaf has been used for a wide range of therapeutic applications including skin diseases and cancer. In this study, we investigated the in vitro cytotoxicity of aqueous and ethanolic extracts of Carica papaya leaves on the human oral squamous cell carcinoma SCC25 cell line in parallel with non-cancerous human keratinocyte HaCaT cells. Two out of four extracts showed a significantly selective effect towards the cancer cells and were found to contain high levels of phenolic and flavonoid compounds. The chromatographic and mass spectrometric profiles of the extracts obtained with Ultra High Performance Liquid Chromatography-Quadrupole Time of Flight-Mass Spectrometry were used to tentatively identify the bioactive compounds using comparative analysis. The principal compounds identified were flavonoids or flavonoid glycosides, particularly compounds from the kaempferol and quercetin families, of which several have previously been reported to possess anticancer activities. These results confirm that papaya leaf is a potential source of anticancer compounds and warrant further scientific investigation to validate the traditional use of papaya leaf to treat cancer.

  12. Chemical Characterization and in Vitro Cytotoxicity on Squamous Cell Carcinoma Cells of Carica papaya Leaf Extracts.

    Science.gov (United States)

    Nguyen, Thao T; Parat, Marie-Odile; Hodson, Mark P; Pan, Jenny; Shaw, Paul N; Hewavitharana, Amitha K

    2016-01-01

    In traditional medicine, Carica papaya leaf has been used for a wide range of therapeutic applications including skin diseases and cancer. In this study, we investigated the in vitro cytotoxicity of aqueous and ethanolic extracts of Carica papaya leaves on the human oral squamous cell carcinoma SCC25 cell line in parallel with non-cancerous human keratinocyte HaCaT cells. Two out of four extracts showed a significantly selective effect towards the cancer cells and were found to contain high levels of phenolic and flavonoid compounds. The chromatographic and mass spectrometric profiles of the extracts obtained with Ultra High Performance Liquid Chromatography-Quadrupole Time of Flight-Mass Spectrometry were used to tentatively identify the bioactive compounds using comparative analysis. The principal compounds identified were flavonoids or flavonoid glycosides, particularly compounds from the kaempferol and quercetin families, of which several have previously been reported to possess anticancer activities. These results confirm that papaya leaf is a potential source of anticancer compounds and warrant further scientific investigation to validate the traditional use of papaya leaf to treat cancer. PMID:26712788

  13. Biodiesel Production by Enzymatic Transesterification of Papaya Seed Oil and Rambutan Seed Oil

    Directory of Open Access Journals (Sweden)

    C. S. Wong

    2014-12-01

    Full Text Available Biodiesel production from vegetable oil has gained attention as an alternative fuel to minimize the usage of fossil fuels and reduce greenhouse gases pollution. In Malaysia, oils from local fruit seeds of papaya and rambutan are potential feedstock for biodiesel production due to their high lipid contents and easily available. In the present study, papaya and rambutan seed oils were extracted via soxhlet apparatus using n-hexane and the oil yields were in between 34–40%. The extracted oils were subjected to enzymatic transesterification by the immobilized Candida rugosa lipase as a catalyst under room temperature with varies molar ratios of methanol to oil. The highest biodiesel yield for papaya seed oil and rambutan seed oil was found to be 96% and 89% at methanol-to-oil ratios of 6:1 and 8:1, respectively. Results also showed a higher biodiesel yield using lipase immobilized on the magnetic particles as the heterogeneous catalyst compared to the yield obtained using free enzyme as the homogeneous catalyst. The properties of biodiesel such as density, acid value, iodine value and cetane number were analyzed and found to meet the European Standard of Biodiesel. The study shows that papaya and rambutan seed oils have the potential to be used as alternative feedstock for biodiesel production than the full dependence on palm oil in Malaysia.

  14. Induction of Resistance to Papaya Black Spot Elicited by Acibenzolar-S-Methyl

    Directory of Open Access Journals (Sweden)

    A.A.R. Oliveira

    2014-01-01

    Full Text Available The objective of this study was to evaluate the effect of acibenzolar-S-methyl tested at 5 concentrations (0, 1, 5, 25 and 100 ?M a.i. for its ability to protect papaya (Carica papaya cv. Rainbow from black spot (Asperisporium caricae following inoculation with the fungus. Effects of resistance induction treatment against black spot disease were evaluated by measuring the plant height and stem diameter. Disease symptoms were scored weekly by visually estimating disease severity of plants on the basis of a 5-class visual scale of 0 (no symptoms to 4 (extensive lesions on leaves. Accumulation of defence-related proteins in papaya leaves were also analysed and compared. Results revealed that the level of protection against A. caricae was dose-dependent. Maximum reduction of the disease in leaves was obtained with 25-100 ?M acibenzolar-S-methyl, with a time interval of 3 days between application of the activator and inoculation with the pathogen. The systemic resistance elicitation was characterized by an increase in 2 pathogenesis-related proteins, chitinase and ?-1, 3-glucanase. These results indicate that acibenzolar-S-methyl induces partial resistance in papaya against black spot disease which may provide the grower a new option for integrated management of the disease.

  15. Injúria pelo frio em frutos de mamoeiro (Carica papaya L. cv 'Golden' Chilling injury in papaya fruits (Carica papaya L. cv 'Golden'

    Directory of Open Access Journals (Sweden)

    Robson Ferreira de Almeida

    2005-04-01

    Full Text Available Os sintomas da injúria pelo frio em frutos de mamoeiro cv 'Golden' foram investigados neste trabalho.Os frutos apresentando de 10% a 15% de coloração amarela na casca, foram classificados na linha de operação de embalagem da Caliman Agrícola S.A (Linhares-ES e após receberam tratamentos térmicos e químicos. Os frutos foram embalados com filmes plásticos e estocados a 6ºC e 13ºC (85-95% UR em uma incubadora (BOD por 30 dias. Em intervalos de tempo definidos, seis frutos foram analisados quanto à firmeza, mudança de cor e aparência. A firmeza foi analisada tanto na região mais externa quanto na região mais interna do mesocarpo. De acordo com os resultados encontrados, os frutos estocados a 6ºC apresentaram os sintomas de escurecimento da casca entre o sexto e o décimo segundo dia de estocagem e também não perderam a cor verde após trinta dias de estocagem, conforme identificado pelos parâmetros de Hunter L (luminosidade, Hunter a (degradação da clorofila e Hunter b (amarelecimento. A firmeza foi drasticamente reduzida nos seis primeiros dias de estocagem nas duas temperaturas. Contudo, ela permaneceu mais elevada na parte externa do mesocarpo durante todo o período de estocagem à 13ºC. Os frutos estocados à 6ºC mostraram um aumento na firmeza, nas duas partes do mesocarpo, entre o sexto e o décimo oitavo dia de armazenamento devido à incidência da injúria pelo frio.The symptoms of chilling injury in papaya fruit cv -Golden' were investigated in this work. Fruits presenting 10% to 15% of fruit skin yellowing were sorted in the packaging line of the Caliman Agrícola S.A (Linhares-ES, after received chemical and heat treatments. The fruits were wrapped in a plastic film and stored at temperatures of 6ºC and 13ºC (85-95% RH in an incubator (BOD for 30 days. At defined time intervals, six fruits were evaluated regarding to firmness, appearance and color change. The fruit firmness was evaluated as in the outer as in the inner mesocarp halves. According to the results found, the fruits stored at 6ºC presented skin scald symptoms between six and twelve days of storage and failure to ripen after thirty days, as identified by the Hunter L (luminosity, Hunter a (loss of chlorophyll and Hunter b (yellowing. The firmness showed a high decrease in the six days of storage in both storage temperatures. However, it stayed higher in the outer mesocarp during all the storage period at 13ºC. Fruits stored at 6ºC showed an increment in firmness in both mesocarp halves between six and eighteen days of storage due to the chilling injury incidence.

  16. Efficacy of Essential Oils on the Conidial Germination, Growth of Colletotrichum gloeosporioides (Penz.) Penz. and Sacc and Control of Postharvest Diseases in Papaya (Carica papaya L.)

    OpenAIRE

    Laura Leticia Barrera-Necha; Silvia Bautista-Banos; Hilda Elizabeth Flores-Moctezuma; Abel Rojas Estudillo

    2008-01-01

    The efficacy of nine essential oils were investigated in vitro on conidial germination and mycelial growth inhibition of Colletotrichum gloeosporioides isolate from papaya (Carica papaya L.). In general, better antifungal effect was observed with Cinnamomum zeylanicum and Syzygium aromaticum oils which had strong inhibition of conidial germination of C. gloeosporioides at 50, 100, 150, 200 and 250 μg mL-1 and a dose dependent inhibition mycelial growth was caused by these oils. Teloxys...

  17. Green synthesis and antibacterial activity screening of silver nanoparticles reduced by papaya (Carica papaya L.) leaves extract

    International Nuclear Information System (INIS)

    The field of nano technology is the most active area of research in modern material sciences. Though there are many chemical, as well as physical methods, green synthesis is the most emerging method of synthesis. This study aimed to describe a cost effective and environment friendly technique for green synthesis of silver nanoparticles. The synthesis of silver nanoparticles was prepared by adding Carica papaya L. leaves extract to 1mM silver nitrate solution. The color change in reaction mixture (pale yellow to dark brown color was observed during the incubation period , due to excitation of surface plasmon vibrations in silver nanoparticles. Nanoparticles were characterized using UV-Visible absorption spectroscopy, X-Ray Diffraction (XRD) pattern, Scanning Electron Microscopy (SEM) and Energy-Dispersive Spectroscopy (EDX) analysis. Absorption spectra of silver nanoparticles formed in the reaction media has absorbance peak at 280 nm, broadening of peak indicates that the particles are poly dispersed. SEM analysis described the morphology and the size of the particles. XRD confirmed the crystalline structure of the nanoparticles. The presence of the elemental silver was observed in the graph obtained from EDX analysis, which also supports the XRD results. The biomass of plants produces their nano materials by a process called bio mineralization. The tests cultures included in the study were Staphylococcus aureus, Escherichia coli and Salmonella. Results showed that the maximum inhibitory effect using 1mM silver nitrates against the microbes were obtained. The approach of plant-mediated synthesis appears to be cost efficient, eco-friendly and easy alternative to conventional methods of silver nanoparticles synthesis (author)

  18. Evaluation of the Combination of Vitamin D3 and Papaya Leaf on Muscle Antioxidant Activity of Spent Chicken

    Directory of Open Access Journals (Sweden)

    A.Q. Sazili

    2011-01-01

    Full Text Available Eighty spent chickens were employed in this study to assay the effect of combination of vitamin D3 and papaya leaf on antioxidant activity of meat in spent layer hens. Diets were a corn-soybean meal based diet for finisher layer with and without vitamin D3 which was supplemented with different levels of 0, 0.5, 1 and 2% for papaya leaf meal. Experiment lasted for 21 days. At day 0, 7, 14 and 21, the birds were scarified and breast muscle was obtained to determine antioxidant activity. Antioxidant activity was measured using 1, 1-Diphenyl-2-Picrylhydrazyl (DPPH. Result obtained from this study demonstrated that antioxidant activity of meat showed remarkable improvement between dietary treatments fed mix of vitamin D3 and papaya leaf and control group. In conclusion, vitamin D3 and papaya leaf when combined indicated an improvement in antioxidant activity of the spent meat.

  19. The use of sodium alginate-based coating and cellulose acetate in papaya post-harvest preservation

    Directory of Open Access Journals (Sweden)

    Denise Andrade Silva

    2014-02-01

    Full Text Available This study aimed to evaluate the ripening of papaya fruit (Carica papaya L. at room temperature (±25°C and10°C with 80% relative humidity, coated with edible film based on sodium alginate (1% and cellulose acetate film (3% by dipping the fruit in the suspensions for 1 min. On the application of the treatment and every three days during 12 days of storage, fruit were evaluated for weight loss, firmness, total carotenoid content, lycopene content and vitamin C content of the pulp. The cellulose acetate film extended the shelf-life of papayas, without affecting their quality. This treatment delayed fruit ripening, whose changes in all the parameters analyzed were significantly slower than fruit treated with sodium alginate-based coating. The coating with cellulose acetate at 3% was more effective in the preservation of papaya stored for 12 days under both temperatures.

  20. Ultrasound-Assisted Extraction (UAE) and Solvent Extraction of Papaya Seed Oil: Yield, Fatty Acid Composition and Triacylglycerol Profile

    OpenAIRE

    Hasanah Mohd Ghazali; Chin Ping Tan; Hamed Mirhosseini; Shadi Samaram

    2013-01-01

    The main objective of the current work was to evaluate the suitability of ultrasound-assisted extraction (UAE) for the recovery of oil from papaya seed as compared to conventional extraction techniques (i.e., Soxhlet extraction (SXE) and solvent extraction (SE)). In the present study, the recovery yield, fatty acid composition and triacylglycerol profile of papaya seed oil obtained from different extraction methods and conditions were compared. Results indicated that both solvent extraction (...

  1. Protective role of Carica papaya (Linn.) in electron beam radiation induced hematological and cytogenetic damages in Swiss albino mice

    International Nuclear Information System (INIS)

    Carica papaya (Linn.) is known to possess various biomedical applications. It has remarkable antioxidant properties. The main objective of the study was to evaluate the leaf extracts of Carica papaya (Linn.) on hematologic and cytogenetic changes occurring due to irradiation of mice to sub-lethal doses of Electron Beam Radiation (EBR). Analysis of hematological changes occurring due to irradiation of mice to sub-lethal doses of EBR, and the effects of Carica papaya (Linn.) extract on the same. The Assessment of hematopoietic stress by spleen colony forming unit and spleen body weight index. The analysis of cell proliferation and immunomodulation with response to the effects of Carica papaya (Linn.) extract by estimation of IL-6. The estimation of serum total antioxidants, lipid peroxidation and analyzing the activities of enzymes like SOD, ALP, and AST. Male Swiss albino mice were fed orally with papaya aqueous leaf extract for 15 days. They were irradiated with a whole body dose of 6 Gy Electron Beam radiation. The mice were dissected for liver, kidney, bone marrow, spleen and brain. The hematological studies were done using blood cell count in an automated cell counter. The biochemical estimations like urea, creatinine, SGOT, SGPT, Total Protein, Albumin, Bilirubin were done using the serum and homogenates. The total antioxidant capacity, the antioxidant enzymes were estimated. The Interleukin-6 levels were estimated in serum to assess immune modulation. The results show a decrease in the hematological parameters in radiated animals. The papaya treated groups have shown modulation in the hematological parameters. The extract has also reduced the suppression of the bone marrow induced by radiation. The radiation induced liver damage is also reduced in papaya treated groups. The aqueous extract of Carica papaya (Linn.) has shown protective effects in electron beam radiation induced tissue damages in Swiss Albino mice (author)

  2. Current status of tropical fruit breeding and genetics for three tropical fruit species cultivated in Japan: pineapple, mango, and papaya

    OpenAIRE

    Ogata, Tatsushi; Yamanaka, Shinsuke; Shoda, Moriyuki; Urasaki, Naoya; Yamamoto, Toshiya

    2016-01-01

    Tropical fruit crops are predominantly produced in tropical and subtropical developing countries, but some are now grown in southern Japan. Pineapple (Ananas comosus), mango (Mangifera indica) and papaya (Carica papaya) are major tropical fruits cultivated in Japan. Modern, well-organized breeding systems have not yet been developed for most tropical fruit species. Most parts of Japan are in the temperate climate zone, but some southern areas such as the Ryukyu Islands, which stretch from Kyu...

  3. Ultrasound-Assisted Extraction (UAE and Solvent Extraction of Papaya Seed Oil: Yield, Fatty Acid Composition and Triacylglycerol Profile

    Directory of Open Access Journals (Sweden)

    Hasanah Mohd Ghazali

    2013-10-01

    Full Text Available The main objective of the current work was to evaluate the suitability of ultrasound-assisted extraction (UAE for the recovery of oil from papaya seed as compared to conventional extraction techniques (i.e., Soxhlet extraction (SXE and solvent extraction (SE. In the present study, the recovery yield, fatty acid composition and triacylglycerol profile of papaya seed oil obtained from different extraction methods and conditions were compared. Results indicated that both solvent extraction (SE, 12 h/25 °C and ultrasound-assisted extraction (UAE methods recovered relatively high yields (79.1% and 76.1% of total oil content, respectively. Analysis of fatty acid composition revealed that the predominant fatty acids in papaya seed oil were oleic (18:1, 70.5%–74.7%, palmitic (16:0, 14.9%–17.9%, stearic (18:0, 4.50%–5.25%, and linoleic acid (18:2, 3.63%–4.6%. Moreover, the most abundant triacylglycerols of papaya seed oil were triolein (OOO, palmitoyl diolein (POO and stearoyl oleoyl linolein (SOL. In this study, ultrasound-assisted extraction (UAE significantly (p < 0.05 influenced the triacylglycerol profile of papaya seed oil, but no significant differences were observed in the fatty acid composition of papaya seed oil extracted by different extraction methods (SXE, SE and UAE and conditions.

  4. Ultrasound-assisted extraction (UAE) and solvent extraction of papaya seed oil: yield, fatty acid composition and triacylglycerol profile.

    Science.gov (United States)

    Samaram, Shadi; Mirhosseini, Hamed; Tan, Chin Ping; Ghazali, Hasanah Mohd

    2013-01-01

    The main objective of the current work was to evaluate the suitability of ultrasound-assisted extraction (UAE) for the recovery of oil from papaya seed as compared to conventional extraction techniques (i.e., Soxhlet extraction (SXE) and solvent extraction (SE)). In the present study, the recovery yield, fatty acid composition and triacylglycerol profile of papaya seed oil obtained from different extraction methods and conditions were compared. Results indicated that both solvent extraction (SE, 12 h/25 °C) and ultrasound-assisted extraction (UAE) methods recovered relatively high yields (79.1% and 76.1% of total oil content, respectively). Analysis of fatty acid composition revealed that the predominant fatty acids in papaya seed oil were oleic (18:1, 70.5%-74.7%), palmitic (16:0, 14.9%-17.9%), stearic (18:0, 4.50%-5.25%), and linoleic acid (18:2, 3.63%-4.6%). Moreover, the most abundant triacylglycerols of papaya seed oil were triolein (OOO), palmitoyl diolein (POO) and stearoyl oleoyl linolein (SOL). In this study, ultrasound-assisted extraction (UAE) significantly (p < 0.05) influenced the triacylglycerol profile of papaya seed oil, but no significant differences were observed in the fatty acid composition of papaya seed oil extracted by different extraction methods (SXE, SE and UAE) and conditions. PMID:24152670

  5. Comparisons of Production Costs and Profit of Three Different Technology Levels of Papaya Production in Tabasco, Mexico

    Directory of Open Access Journals (Sweden)

    E. Guzmn-Ramn

    2008-04-01

    Full Text Available The survey was carried out from September 2006 to January 2007 in three papaya production sites located in main papaya production zones in Tabasco; SE Mexico. There are differences in size of the cultivated area, in the yield of the papaya as well as in production costs and profit, according to the different technology levels in the farming systems: low, medium and high technology cultivation level. The financial evaluations were carried out in three sites with different productive technologies. The comparison of the agronomic and economic traits results for low technology level in: V AN of 2359.00 USD, BCR in 1.9 and an equilibrium point of 3750.00 USD, TIR of 0.25. In order to avoid loses, a quantity of 10714 kg papaya should be sold. In medium technology VAN is 1605.10 USD, BCR is 1.7, TIR 0.20 and the equilibrium point is 12800.00 USD. 36571 kg of papaya should be yearly sold. In high technology level VAN is 11749.40, BCR is 2.73, TIR 0.43 and the equilibrium point is 12187.50 USD, 34821 kg papaya should be sold yearly. The indicators showed that all three levels are profitable and economically viable.

  6. Population dynamics and within-plant distribution of the mite Calacarus flagelliseta (Acari: Eriophyidae) on papaya in Hawaii.

    Science.gov (United States)

    Fournier, Valerie; Rosenheim, Jay A; Brodeur, Jacques; Johnson, Marshall W

    2004-10-01

    An important element in developing a management strategy for a new pest is the study of its seasonal dynamics and within-plant distribution. Here, we studied the mite Calacarus flagelliseta Fletchmann, De Moraes & Barbosa on papaya, Papaya carica L. (Caricaceae), in Hawaii to quantify 1) patterns of seasonal abundance, 2) its distribution across different vertical strata of the papaya canopy, and 3) shifts in its use of the upper versus the lower surfaces of papaya leaves. Nondestructive sampling conducted in two papaya plantings revealed that 1) populations of C. flagelliseta peak during the summer; 2) mites are most abundant in the middle and lower strata of the plant canopy, and least abundant on the youngest leaves found in the upper canopy; and 3) mites are found more predominantly on the upper leaf surfaces when overall population density peaks, suggesting that individuals move from the lower to the upper leaf surfaces when food resources on the lower leaf surface have been exploited by conspecifics. These results have significant implications for the development of sampling plans for C. flagelliseta in papaya. PMID:15568344

  7. La historia de la Iglesia en la historiografa chilena 1965-2015

    Directory of Open Access Journals (Sweden)

    Douglas Barry W.

    2015-01-01

    Full Text Available La historia de la Iglesia en Chile ha sido tra - tada desde mltiples perspectivas, tanto por historiado - res eclesisticos como civiles. Igualmente variadas son las temticas abordadas por dichos autores, que refle - jan la transversalidad de la incidencia e influencia de la Iglesia en distintos mbitos de la vida nacional tanto en el periodo hispano, en el proceso de Independencia y en su vida republicana. En el presente artculo se exponen las principales aportaciones que en los ltimos cincuen - ta aos ha brindado la historiografa chilena a la historia de la Iglesia, de acuerdo a una seleccin de temas que vienen a completar los presentados en la primera parte de este trabajo, tales como la recopilacin y transcrip - cin de archivos eclesisticos, la historia de las iglesias particulares o locales, los aspectos jurdico eclesisticos y las manifestaciones culturales y artsticas.

  8. La ambivalente representación del roto en la poesía popular chilena

    Directory of Open Access Journals (Sweden)

    Pamela Tala Ruiz

    2011-11-01

    Full Text Available Este artículo examina la representación ambivalente de la figura del roto en la poesía popular chilena impresa en hojas sueltas entre fines del siglo XIX y principios del XX. Se estudia la manera en que se construye desde la cultura popular la celebración del roto en tanto representante de la nación (el soldado anónimo que lucha contra la amenaza extranjera, por un lado, y un rechazo en su mismo grupo social (en tanto se le asocian significados como vagancia, robo, inestabilidad, por otro. Se comprueba que la representación del roto como sujeto de la enunciación de estos poemas es un ejemplo más de su compleja identidad.

  9. Creencias y prácticas de literacidad en familias chilenas con distintos niveles educativos

    Directory of Open Access Journals (Sweden)

    Lissi María Rosa

    2007-01-01

    Full Text Available El estudio describe las creencias y prácticas de literacidad de familias con distinta educación en Chile. Participaron 188 niños y niñas de kindergarten y sus familias. Los padres contestaronun cuestionario acerca de su nivel educacional, ciertas prácticas de literacidcd familiar, y sus creencias acerca del desarrollo de ésta. Los resultados muestran diferencias y similitudes con resultados de países desarrollados. Las principales diferencias se dan en la frecuencia de ciertas prácticas, las que ocurren con menor frecuencia en las familias chilenas, independientemente del nivel educacional. Las principales similitudes con los hallazgos de países desarrollados se dan en las asociaciones entre el nivel educacional de la familia y las variables de literacidad familiar

  10. Tendencias en la historiografía eclesiástica Chilena

    Directory of Open Access Journals (Sweden)

    Camus Ibacache, Misael

    2001-12-01

    Full Text Available The article studies the lines of Chilean ecclesiastical history in XIX and XX. During both centuries the studies are centered in: Independence, Church-State relations, missions, editions of sinodal and pastoral texts. The A. values the influence of the liberal historiography in ecclesiastical history and emphasizes the existence of a new current that denominates pastoral historiography and describes the new sources that are used.

    Estudia las corrientes de la historia eclesiástica chilena en los siglos XIX y XX. Durante ambos siglos los estudios se centran en la Independencia, relaciones Iglesia-Estado, misiones, ediciones de textos conciliares y ediciones de textos pastorales. El A. valora la influencia de la historiografía liberal en la historia eclesiástica y destaca la existencia de una nueva corriente que denomina historiografía pastoral y describe sus fuentes.

  11. Turismo sostenible y proyectos hidroeléctricos: contradicciones en la patagonia chilena

    Directory of Open Access Journals (Sweden)

    Gabriel Inostroza

    2014-01-01

    Full Text Available El artículo analiza las perspectivas del turismo sostenible en la Patagonia chilena en relación a la probable implantación de centrales hidroeléctricas en su territorio. Se estudia el impacto de un gran proyecto hidroeléctrico en la región de Aysén (Chile y su repercusión en el turismo. Se concluye que la construcción y operación de grandes embalses y presas con sus correspondientes líneas de trasmisión eléctrica atentarían contra la principal ventaja competitiva del sector turístico de la Patagonia: una naturaleza que aún se conserva y que se ha proyectado en el imaginario social.

  12. Evolucin de la industria chilena de fondos mutuos. Anlisis de su performance (2000-2005

    Directory of Open Access Journals (Sweden)

    Benito Umaa Hermosilla

    2013-05-01

    Full Text Available En el presente estudio, los autores desarrollan un anlisis riguroso de la evolucin de la Industria Chilena de Fondos Mutuos. Se aplican para ello las medidas tradicionales de performance, correspondientes a los autores Sharpe, Treynor y Jensen, poniendo para ello de manifiesto, que su funcionamiento correcto depende de una serie de circunstancias, que no siempre se cumplen en los mercados financieros. En estos casos se aplican medidas alternativas de medicin de la performance, las cuales eliminan las incoherencias detectadas. Estas medidas fueron desarrolladas por los profesores Ferruz y Sarto (1997. El presente estudio comprende el periodo 2000- 2005. Se analizan rentabilidades mensuales correspondientes a 196 fondos mutuos de renta variable. Los ndices alternativos no hicieron ms que ratificar el resultado de los ndices originales. Se concluye adems que las gestoras con mayor patrimonio administrado no poseen los mejores ndices de performance, similar situacin ocurre con aquellas gestoras pertenecientes a la industria bancaria.

  13. Interacciones tempranas y género infantil en familias monoparentales chilenas

    OpenAIRE

    Marcia Olhaberry Huber

    2012-01-01

    Se realizó un estudio transversal de las interacciones madre-hijo(a) en familias monoparentales chilenas de bajo ingresos, considerando las diferencias según el género infantil. Participan 80 díadas, con 39 niñas y 41 niños entre los 4 y 15 meses y se evalúa la calidad de las interacciones con el instrumento CARE-Index. Se observan altos puntajes en depresión y estrés materno, así como un promedio en la calidad de la interacción que indica necesidad de intervención, mostrando lo anterior vuln...

  14. Literatura chilena: canonización e identidades Chilean literature: canonization and identities

    Directory of Open Access Journals (Sweden)

    Iván Carrasco M.

    2005-09-01

    Full Text Available Este trabajo expone los principales problemas y fundamentos teóricos de la literatura chilena concebida como resultado de procesos de canonización relacionados con procesos de construcción de identidades y propone la existencia de dos clases de espacios de escritura: la literatura canonizada y la literatura marginal, representada por tres manifestaciones de carácter intercultural: la poesía etnocultural, la literatura del exilio y la escritura de inmigrantesThis paper exposes the main problems and theoretical foundations of the Chilean Literature conceived as result of canonization processes and proposes the existence of two writing spaces: the canonized literature and the marginal literature, represented by three intercultural manifestations: the ethnocultural poetry, the literature of exile and the writing of the immigrants

  15. Toponimia chilena: "la lejana posesión" Chilean toponymy: the far-away possession

    Directory of Open Access Journals (Sweden)

    Guillermo Latorre

    2001-01-01

    Full Text Available La toponimia de la Isla de Pascua está casi totalmente dominada por vocablos pascuenses. Este predominio ha aumentado a pesar de las expediciones holandesas, españolas e inglesas durante el siglo XVIII, las misiones religiosas francesas, las asonadas esclavistas desde Perú y la toma de posesión y administración chilenas durante el siglo siguiente. Estas visitas, muy especialmente la de la expedición española de 1770, deberían haber extinguido los topónimos rapa nui, como ha sucedido con otras lenguas autóctonas en Chile. Muy por el contrario, muchos nombres pascuenses ya se habían establecido a mediados del siglo XIX y con la administración chilena se ha producido un aumento en la toponimia vernácula, hasta el punto de que ella es dominante en la actualidad. La supervivencia del rapa nui como lengua viva es solamente uno de los factores que explican este predominio.The toponymy of Easter Island is almost completely dominated by names in Rapa Nui, the native language. This dominance has increased in spite of the Dutch, Spanish and English expeditions during the eighteenth century as well as the French religious missions, the slave raids from Peru , and the Chilean administration of the island during the following century. All of these, particularly the Spanish expedition of 1770, should have resulted in the extinction of the Rapa Nui toponymy, as has happened with other native languages in Chile. Quite the contrary, many native place names were already established by the middle of the nineteenth century; under Chilean administration there has been an increase in the vernacular place names, to such an extent that the latter are dominant at present. The survival of Rapa Nui as a living language is just one of the factors accounting for this dominance.

  16. Implementación del enfoque de derechos humanos: la reforma sanitaria chilena

    Directory of Open Access Journals (Sweden)

    Nuria Cunill-Grau

    2014-01-01

    Full Text Available EI propósito de este trabajo es analizar la incidencia de la arquitectura institucional publica en la implementación de las políticas públicas con enfoque de derechos humanos. Se adopta como caso de estudio la reforma sanitaria chilena a partir del año 2005, que reconoce garantías exigibles de acceso, oportunidad, calidad y protección financiera para un conjunto de problemas de salud a la ciudadanía, independientemente de su adscripción al sistema público 0 privado. La metodología integró un análisis cualitativo de normas legales, instrumentos de gestión y entrevistas en profundidad principalmente a directivos de salud. Los resultados muestran que, no obstante los evidentes logros de la reforma chilena en pos de la equidad en salud, resultan limitados sus esfuerzos de dotar de gobernanza sistémica, accountability y espacio público a su arquitectura institucional, comprometiendo con ello su intención de instaurar un enfoque de derechos humanos. Se concluye que el patrón hegemónico de diseño institucional, organización y gestión de los servicios sociales requiere ser enfrentado para construirviabilidad a la implementación de este tipo de política y se sugiere la importancia de adoptar estrategias diferenciadas de accountabitily que consideren las asimetrías sociales en la apropiación y exigibilidad de los derechos.

  17. Anatomia foliar de plantas transgênicas e não transgênicas de Carica papaya L. (Caricaceae Leaf anatomy of genetically modified and wild-type Carica papaya L. (Caricaceae

    Directory of Open Access Journals (Sweden)

    Marcos Vinicius Leal-Costa

    2010-06-01

    Full Text Available O mamoeiro, Carica papaya L. (Caricaceae é uma espécie americana, cujos frutos são largamente consumidos em todo mundo. Devido às perdas de produção provocadas por viroses e a dificuldade em controlá-las por métodos convencionais, a espécie tem sido alvo de pesquisas de melhoramento genético envolvendo transgenia para resistência a vírus. O presente trabalho descreve a anatomia foliar de plantas de mamoeiro convencional e transgênico resistente ao vírus da mancha anelar-Papaya ringspot virus (PRSV com inserção da capa protéica viral. As duas cultivares apresentam pecíolo com endoderme e fibras pericíclicas. As folhas são hipoestomáticas e dorsiventrais, com laticíferos acompanhando os feixes vasculares e grande concentração de idioblastos com drusas de oxalato de cálcio. A epiderme é glabra, possuindo estômatos anomocíticos e anisocíticos, com células de paredes anticlinais retas na face adaxial e levemente sinuosas na face abaxial. O presente trabalho concluiu que o processo de transformação genética não alterou as características anatômicas das folhas de C. papaya, servindo de subsídio para avaliação da conformidade anatômica da cultivar transgênica.Papaya, Carica papaya L. (Caricaceae, is an American species, consumed worldwide. A major limitation to papaya production is attack by viruses, like the papaya ringspot virus (PRSV. Papaya has been genetically modified to increase its resistance to PRSV. The aim of this research was to compare the leaf anatomy of wild-type and genetically modified (GM C. papaya plants to evaluate the influence of genetic modification on leaf anatomy. Wild-type and GM plants showed petiole with endodermis and pericycle fibers. The leaves are hypostomatic and dorsiventral, with laticifers along vascular system and abundant druses of calcium oxalate. The epidermis was glabrous and presented anomocytic and anisocytic stomata, straight anticlinal walls on the adaxial face and sinuous on the abaxial face. Anatomical differences between wild-type and GM C. papaya leaves were not observed. These data contribute to risk assessments regarding the anatomical conformity of GM plants.

  18. Sex and aggregation pheromone transport after methyl eugenol consumption in male Bactrocera papayae

    International Nuclear Information System (INIS)

    Amongst at least 52 sibling species complexes in the Oriental fruit fly, Bactrocera dorsalis Hendel (Diptera: Tephritidae), B. papayae (formerly Mal B) Drew and Hancock (Drew and Hancock 1994) is beginning to emerge as an economically important insect pest which poses a severe threat to the fruit cultivation in both subtropical and tropical countries. In Malaysia, B. papayae is one of the most damaging pests which infests many commercially grown fruits (Tan and Lee 1982). Like the Oriental fruit fly and its sibling species complex, B. carambolae Drew and Hancock, B. papayae is also strongly attracted to, and compulsively feeds on, methyl eugenol (ME) (Tan 1993). Chemical analyses revealed that in B. papayae males, ME is converted to phenylpropanoids which are then selectively accumulated in the rectal gland. Of the three major volatile substances, 2-allyl-4,5-dimethoyphenol (allyl-DMP) was detected in higher quantities relative to the trans-coniferyl alcohol (4-(3-hydroxy-E-propenyl)-2-methoxyphenol) (CF) and cis-3,4-dimethoxycinnamyl alcohol (cis-DMC) (Nishida et al. 1988a, 1988b). Behavioural studies have also shown that allyl-DMP and CF function as male sex and aggregation pheromone in B. papayae (Tan and Nishida 1996, Hee and Tan 1998). Allyl-DMP was found to be the most attractive compound and cis-DMC the least attractive to the males (Tan 1996). Consumption of ME enhances the mating competitiveness of males. This is demonstrated by the strong attraction of females to conspecific ME-fed males in wind tunnel experiments (Hee and Tan 1998). In male-male mating competition for virgin females, males that fed on ME performed significantly better (Shelly and Dewire 1994, Tan and Nishida 1996). Thus it appears that ME-fed males produced signals that were more attractive. However, the characterisation and understanding of the functions of these phenylpropanoids have not been accompanied by studies of their physiological mode of transport in male flies. The current paper describes the sex and aggregation pheromone transport after methyl eugenol consumption in male B. papayae. Here, we investigate the presence of the pheromones and their precursor ME in the male digestive and circulatory system

  19. Radiation induced mutation to develop dwarf and precocious lines of papaya

    International Nuclear Information System (INIS)

    Papaya (Carica papaya L.) is one of the most important fruit cultivated throughout the tropical and subtropical regions of the country. Almost all the varieties of papaya developed either through selection or hybridization. As regards mutation breeding, very little work has been done in India. Only one variety PusaNanha has been developed through mutation breeding by treating the seed of papaya strain Pusa 1-15 with 15Kr gamma rays. Mutation breeding is the most appropriate for improving one or two easily identifiable traits in an otherwise well accepted breeding lines or commercial variety. Dwarfness and earliness in flowering are important traits in high density planting as breeding objective for improving papaya varieties for high yield with medium size fruits and good fruit quality. With these objectives, seeds of the papaya P 7-2 were treated with gamma rays 0.1, 0.15, 0.2, 0.25 and 0.3 kGy. The control recorded maximum germination (68%). Among the other treatments maximum germination (64.25%) was recorded at 0.1 kGy. The germination percentage was observed to be least (45%) at 0.3 kGy. Minimum length of seedlings (8.5 cm) and diameter (3.92 mm) was recorded in treatment 0.1 kGy while maximum length (19.2 cm) and diameter (6.26 mm) was recorded in treatment 0.3 kGy after 30 days of sowing. Minimum height of the plant (79.24 cm) was recorded in treatment 0.1 kGy while maximum (112.20) in control. Minimum plant girth (33.40 cm) was measured in 0.3 kGy while maximum (44.34 cm) in 0.15 kGy treatment. Minimum height at first flower initiation (55.28 cm), days to flower initiation (78.28) and length of petiole (60.45 cm) was noted in treatment 0.1 kGy while maximum height at first flower initiation (78.2 cm), days to flower initiation (87.46) and length of petiole (68.24 cm) was found in control. Among treatments, maximum number (18) of fruit was counted in 0.3 kGy treatment while maximum weight of fruit (750 g) was recorded in control. Maximum TSS (10.6 °Brix) in fruit was found in treatment 0.1 kGy. Dwarf and precocious lines were selected and selfed for raising M2 population. (author)

  20. Control de dos especies de Colletotrichum causantes de antracnosis en frutos de papaya Maradol Control of two species of Colletotrichum causing anthracnose in Maradol papaya fruits

    Directory of Open Access Journals (Sweden)

    Felipe Santamaría Basulto

    2011-10-01

    Full Text Available La antracnosis es una limitante de la vida útil de los frutos de papaya, es causada principalmente por Colletotrichum gloeosporioides, aunque es posible encontrar otras especies de Colletotrichum causando enfermedades en un mismo cultivo. Para el control de la antracnosis en Yucatán se recomiendan varios fungicidas, pero no todos están permitidos por la agencia de protección al ambiente (EPA de los Estados Unidos de América, principal país al cual se exporta la papaya yucateca. El presente estudio tuvo como objetivos identificar el agente causal de la antracnosis de papaya en Yucatán, evaluar el efecto de fungicidas que cuentan con registro de EPA sobre las especies que causan antracnosis y explorar el efecto de inductores a resistencia en el control de esta enfermedad. Durante marzo y mayo de 2006 se obtuvieron nueve aislamientos de Colletotrichum en frutos de tres regiones productoras de Yucatán, los cuales se identificaron mediante claves taxonómicas. La evaluación de los productos se hizo mediante bioensayos de sensibilidad in vitro y la efectividad en frutos inoculados, posteriormente en agosto de 2007, noviembre de 2007 y febrero de 2008, se evaluó el efecto de la aplicación poscosecha de los fungicidas e inductores de resistencia sobre la antracnosis en frutos infectados naturalmente desde campo. En las tres localidades se encontraron dos especies que causan antracnosis identificadas como C. gloeosporioides y C. dematium. En condiciones in vitro el desarrollo de C. gloeosporioides fue inhibido por prochloraz, ferbam, azoxystrobin, tryfloxystrobin y clorotalonil; el desarrollo de C. dematium fue inhibido sólo por prochloraz, ferbam y clorotalonil. En frutos inoculados prochloraz tuvo 100% de efectividad para las dos especies, mientras que azoxystrobin tuvo 87.5% de efectividad para C. gloeosporioides y 3.3% para C. dematium.Anthracnose is a limitation of papaya fruits' life, mainly caused by Colletotrichum gloeosporioides, although it is possible to find other Colletotrichum species causing diseases in the same crop. In order to control anthracnose in Yucatán, several fungicides are recommended, but not all of them are allowed by the Environmental Protection Agency (EPA of the United States, the main country to which Yucatan's papaya is exported. This study aimed to identify the causal agent of anthracnose of papaya in Yucatan, to evaluate the effect of fungicides that have EPA registration on species that cause anthracnose and to explore the effect of resistance inducers in controlling this disease. During March and May 2006, nine isolates of Colletotrichum were obtained from fruits of three producing regions of Yucatán, which were identifed using taxonomic keys. The product evaluation was done by sensitivity in vitro bioassays and the effectiveness in inoculated fruits, ensuing in August 2007, November 2007 and February 2008, the effect of postharvest application of fungicides and resistance inducers on anthracnose on naturally infected fruits from the field were evaluated. In the three sites, two species that cause anthracnose were found and identified as C. gloeosporioides and C. dematium. Under in vitro conditions, C. gloeosporioides development was inhibited by prochloraz, ferbam, azoxystrobin, tryfloxystrobin and chlorothalonil; C. dematium development was inhibited only by prochloraz, ferbam and chlorothalonil. In inoculated fruits, prochloraz was 100% effective for both species, while azoxystrobin was 87.5% effective in C. gloeosporioides and 3.3% in C. dematium.

  1. Validacin Chilena del Cuestionario de Evaluacin del Apego Romntico Experiences in Close Relationships (ECR: Anlisis de la Validez de Criterio

    Directory of Open Access Journals (Sweden)

    Rosario Spencer

    2013-01-01

    Full Text Available El objetivo de esta investigacin fue analizar la validez de criterio del cuestionario de apego romntico Ex - periences in Close Relationships (ECR, Brennan, Clark y Shaver, 1998 para la poblacin chilena de adultos. Este cuestionario evala las dos dimensiones del apego romntico adulto: la evitacin (de la intimidad y la ansiedad (acerca de las relaciones. Los participantes fueron 549 adultos, quienes completaron el cuestionario ECR y el cuestionario de apego adulto CaMir. Se evalu las propiedades psicomtricas del cuestionario, mostrando que es un instrumento con una adecuada validez de criterio y con una estructura factorial que representa las dos dimensiones tericas propuestas por los autores originales. Se concluye que la versin chilena del ECR es vlida para examinar el apego romntico en la adultez.

  2. INVENTARIO DE GASES CON EFECTO INVERNADERO EMITIDOS POR LA ACTIVIDAD AGROPECUARIA CHILENA Inventory of greenhouse gas emissions by Chilean agriculture

    OpenAIRE

    Rafael Novoa S. A.; Sergio González M.; Rosemary Novoa J.; Rosa Rojas

    2000-01-01

    Se realizó una estimación de los gases con efecto invernadero emitidos por la agricultura chilena. Los resultados indican que el año 1994, la agricultura chilena emitió 321,92 Gg de metano, 21,80 Gg de N2O; 2,96 Gg de NOx y 51,97 Gg de CO. Además, se estimó que las emisiones de COVNM llegan a 2,59 Gg año-1. Estas cifras expresadas como porcentaje de las emisiones del sector no energía chileno llegan a un 74,3 % para el metano, un 5,1 % para el CO; un 93,8 % para el N2O; un 9,8 % para los NOx ...

  3. Subversión de la figura del mártir en la narrativa chilena contemporánea

    Directory of Open Access Journals (Sweden)

    Claire Mercier

    2013-06-01

    Full Text Available El estudio se acerca a la subversión de la figura tradicional del mártir en la narrativa chilena contemporánea, a partir del análisis de dos novelas: El Desierto de Carlos Franz (2005 y La vida doble de Arturo Fontaine (2010. Se inscriben su trama durante el periodo de la Dictadura Chilena y a través del camino identitario de dos mujeres. Así, se tratará de seguir una huella ambigua y torturada respecto a una inversión de la figura del mártir "convencional", a fin de esbozar los contornos de su recreación por la literatura (y para qué.

  4. Estética, política y vida cotidiana. El caso de la pintura callejera chilena

    OpenAIRE

    Patricio Rodriguez-Plaza

    2005-01-01

    Este artículo trata de compartir una reflexión académica con respecto al fenómeno de la pintura callejera chilena, entendiéndola como un hecho estético total, que lo es menos por sus producciones y resultados que por los usos y percepciones que lo entretejen, conforman y desparraman por la ciudad.

  5. Estética, política y vida cotidiana. El caso de la pintura callejera chilena

    Directory of Open Access Journals (Sweden)

    Patricio Rodriguez-Plaza

    2005-06-01

    Full Text Available Este artículo trata de compartir una reflexión académica con respecto al fenómeno de la pintura callejera chilena, entendiéndola como un hecho estético total, que lo es menos por sus producciones y resultados que por los usos y percepciones que lo entretejen, conforman y desparraman por la ciudad.

  6. Nematodos agalladores asociados al cultivo de papaya (Carica papaya L. en el departamento de Córdoba, Colombia

    Directory of Open Access Journals (Sweden)

    Jaraba Juan de Dios

    2007-06-01

    Full Text Available

    El objetivo de este trabajo fue identificar los nematodos agalladores asociados al cultivo de papaya (Carica papaya L. en los municipios de Tierralta, Valencia, Lorica, Montería y Montelíbano que constituyen el área productora de papaya en Córdoba. Se evaluaron variables morfológicas y morfométricas en hembras, machos y juveniles de segundo estadío (J2. En las muestras de suelo se determinó pH, contenido de materia orgánica (MO, conductividad eléctrica (CE y textura, a fin de correlacionarlas con la presencia de especies nematodas. Se encontraron Meloidogyne javanica,M. incognita y M. arenaria, siendo M. incognita la especie más frecuente. Las tres especies se encontraron mezcladas en 37,5% de los individuos;M. incognita y M. arenaria en 50%, mientras que M. incognita y M. javanica no se encontraron mezcladas. Las especies se encontraron en pH de 4,9 a 6,4; CE de 0,1 a 0,45 dS· m-1; MO de 1,2 % a 2,5 % y la textura fue arenosa, franca y franco arenosa. El pH, el contenido de arena y la CE correlacionan de manera positiva con la presencia de los nematodos, mientras la MO mostró correlación negativa. Se reporta por primera vez la ocurrencia de M. javanica, M. incognita, M. arenaria, así como la mezcla de M. incognita y M. arenaria, en papayas de Colombia.

  7. Caracterización de accesiones de papaya (Carica papaya L.) a través de marcadores AFLP en Cuba

    OpenAIRE

    Maruchi Alonso Esquivel; Martín Bautista Alor; Matilde Ortiz García; Adriana Quiroz Moreno; Wolfgang Rohde; Lorenzo Felipe Sánchez Teyer

    2010-01-01

    Los marcadores moleculares son herramientas valiosas en los estudios genéticos en plantas, y están siendo empleados exitosamente en programas de mejoramiento principalmente en la elección de progenitores y en la selección. El polimorfismo observado mediante la técnica molecular AFLP (Amplified Fragment Length Polymorphism) ha sido de utilidad para estudios de diversidad genética en frutales. En el presente trabajo se realizó la caracterización molecular de 12 accesiones de papaya (Carica papa...

  8. Empleo de un recubrimiento formulado con propóleos para el manejo poscosecha de frutos de papaya (carica papaya l. cv. hawaiana)

    OpenAIRE

    Barrera Bello, Elizabeth; Gil Loaiza, Marcela; García Pajón, Carlos Mario; Durango Restrepo, Diego Luis

    2012-01-01

    Resumen. El fruto de papaya es reconocido por las propiedades nutricionales y sensoriales; no obstante, su tiempo de vida útil en poscosecha es muy corto. En los últimos años, para incrementar la vida de almacenamiento del fruto se ha implementado el uso de recubrimientos como vehículos de agentes antimicrobianos y antioxidantes, entre otros. En este trabajo se comparó el efecto de dos recubrimientos; cera comercial (control) y cera comercial conteniendo un extracto etanólico de propóleos (5%...

  9. Studies on physiologically active root distribution and appropriate method of fertilizer application using isotopic techniques in two varieties of papaya (Carica papaya) during pre-bearing stage

    International Nuclear Information System (INIS)

    In Coorg Honey Dew and Surya varieties of papaya aged 10 weeks, the abundance of active roots at 10 cm depth decreased during 20 to 100 days after injection of carrier-free 32P from 77% to 62% in the former and from 89% to 47% in the latter. Bulk of the active roots (85-65%) resided between 10 to 50 cm radial distance. While the phosphorus derived from fertilizer (Pdff) 32P-labelled superphosphate varied from 0.72 to 10.27%, placement between 10 and 40 cm radial distance was appropriate. (author)

  10. Characterization of the exogenous insert and development of event-specific PCR detection methods for genetically modified Huanong No. 1 papaya.

    Science.gov (United States)

    Guo, Jinchao; Yang, Litao; Liu, Xin; Guan, Xiaoyan; Jiang, Lingxi; Zhang, Dabing

    2009-08-26

    Genetically modified (GM) papaya (Carica papaya L.), Huanong No. 1, was approved for commercialization in Guangdong province, China in 2006, and the development of the Huanong No. 1 papaya detection method is necessary for implementing genetically modified organism (GMO) labeling regulations. In this study, we reported the characterization of the exogenous integration of GM Huanong No. 1 papaya by means of conventional polymerase chain reaction (PCR) and thermal asymmetric interlaced (TAIL)-PCR strategies. The results suggested that one intact copy of the initial construction was integrated in the papaya genome and which probably resulted in one deletion (38 bp in size) of the host genomic DNA. Also, one unintended insertion of a 92 bp truncated NptII fragment was observed at the 5' end of the exogenous insert. Furthermore, we revealed its 5' and 3' flanking sequences between the insert DNA and the papaya genomic DNA, and developed the event-specific qualitative and quantitative PCR assays for GM Huanong No. 1 papaya based on the 5' integration flanking sequence. The relative limit of detection (LOD) of the qualitative PCR assay was about 0.01% in 100 ng of total papaya genomic DNA, corresponding to about 25 copies of papaya haploid genome. In the quantitative PCR, the limits of detection and quantification (LOD and LOQ) were as low as 12.5 and 25 copies of papaya haploid genome, respectively. In practical sample quantification, the quantified biases between the test and true values of three samples ranged from 0.44% to 4.41%. Collectively, we proposed that all of these results are useful for the identification and quantification of Huanong No. 1 papaya and its derivates. PMID:19645503

  11. Fitotoxicidade de fungicidas, acaricidas e inseticidas sobre o mamoeiro (Carica papaya L.) cultivar sunrise solo improved line 72/12 / Toxic effects of pesticides products on papaya plant (Carica papaya L.) cv. sunrise solo improved line 72/12

    Scientific Electronic Library Online (English)

    Alclio, Vieira; Carlos, Ruggiero; Srgio Lucio David, Marin.

    2003-04-01

    Full Text Available Estudaram-se os efeitos fitotxicos de fungicidas, acaricidas e inseticidas e algumas associaes entre eles, em plantas de mamoeiros (Carica papaya L.) cv. Sunrise Solo Improved Line 72/12, em condies de vero, no municpio de So Mateus - ES, localizado na regio produtora do Estado. O experimen [...] to foi arranjado em delineamento de blocos casualizados, com 4 repeties e 03 plantas teis por parcela experimental. Foram utilizados os seguintes produtos, com as respectivas doses, para cada 100 l de gua: abamectin (Vertimec 18 CE - 50 ml); dicofol + tetradiphon (Carbax - 200 ml), fenbutatin oxide (Torque 500 SC - 60 ml); mancozeb (Dithane PM - 200g); oxicloreto de cobre (Reconil - 400g) e thiabendazole (Tecto 450 - 100ml). Analisou-se a fitotoxicidade dos produtos em relao altura da planta, n de folhas, nmero de flores e frutos ; dimetro do caule e queimaduras ou injrias foliares. As medies e contagens foram feitas um dia antes das pulverizaes, 15 e 30 dias aps. Constatou-se que o Vertimec 18 CE, associado ao Reconil ou ao Tecto 450, ocasionou leves injrias foliares, detectadas aos 15 dias aps as pulverizaes, que se tornaram praticamente imperceptveis, aos 30 dias aps as pulverizaes; e que Dithane PM, Reconil, Tecto 450, Carbax, Torque 500 SC, Dithane PM + Carbax, Dithane PM + Torque 500 SC, Dithane PM + Vertimec 18 CE, Reconil + Carbax, Reconil + Torque 500 SC, Tecto 450 + Carbax e, Tecto 450 + + Torque 500 SC no interferiram nos parmetros de desenvolvimento e de produo estudados, bem como no causaram injrias ou queimaduras nas folhas dos mamoeiros. Abstract in english In this work was studied the phytotoxic effect of inseticides, acaricides and fungicides alone and in combination, on papaya plants (Carica papaya L.); cv. Sunrise Solo Improved Line 72/12, under field conditions. The experiment were conducted in a farm, located at So Mateus county, the most import [...] ant papaya region in the State of Esprito Santo.The experiment was arranged in a randomized block design with four replications, with three plants by plot. The products with their respective dose in a volume of 100 l H2O were: abamectin (Vertimec 18 CE - 50 ml); dicofol + tetradifon (Carbax - 200 ml); fenbutatin oxide (Torque 500 SC - 60 ml); mancozeb (Dithane PM - 200g).; cuprix oxychloride (Reconil 400g) and thiabendazole (Tecto 450 - 100ml). Plants were evaluated for plant high, number of leaves, flowers and fruits, and extension of leaf injury, one day before, 15 and 30 days after spray. Vertimec 18 CE in combination with Reconil or Tecto 450, caused light leaf injuries, observed only15 days after spraying. These plants recovered from leaf injury and 30 days later, no leaf lesion was observed. Dithane PM, Reconil, Tecto 450, Carbax, Torque 500 SC, Dithane PM + Carbax , Dithane PM + Torque 500 SC, Dithane PM + Vertimec 18 CE, Reconil + Carbax, Reconil + Torque 500 SC, Tecto 450 + Carbax and Tecto 450 + Torque 500 SC, did not affected plant growth and yield studied. No leaf burns injury were caused in papaya plants.

  12. Uso de esterco associado à adubação mineral na produção de mudas de mamoeiro (Carica papaya L. The use of manure associated with mineral fertilization in papaya (Carica papaya L. seedling production

    Directory of Open Access Journals (Sweden)

    Regina Célia Faria Simão Canesin

    2006-12-01

    Full Text Available Na composição do substrato para produção de mudas de mamoeiro, recomenda-se o uso de adubação orgânica, que traz como vantagens a melhoria das características físicas, químicas e biológicas do solo, criando um ambiente favorável ao desenvolvimento inicial das mudas, bem como boa resposta do mamoeiro. Os materiais orgânicos desempenham ainda um importante papel na nutrição das plantas como fornecedores de nutrientes. Contudo, observa-se que existe uma grande variação nas recomendações e quase sempre a adubação orgânica está associada à adubação mineral. Neste contexto, o objetivo deste trabalho foi o de verificar os efeitos do uso de esterco de curral associado ou não à adubação mineral no substrato sobre a formação de mudas de mamoeiro. O Experimento foi conduzido na FEP/UNESP/Câmpus de Ilha Solteira-SP. O delineamento utilizado foi em blocos, em esquema de parcelas subdivididas, sendo parcelas as cultivares e subparcelas os substratos. Cada parcela com 5 repetições e 10 plantas úteis por subparcela. Para a comparação das médias, utilizou-se o teste de Tukey. Com base nos resultados obtidos, pode-se concluir que: a Na formação das mudas de mamoeiro, o esterco de curral pode ser utilizado sem a necessidade de adubação mineral com o superfosfato simples e o cloreto de potássio; b O esterco de curral foi capaz de fornecer às mudas de mamoeiro os nutrientes N, P, K, Ca, Mg e Cu, necessários para seu desenvolvimento até o transplantio para o campo.The use of organic fertilization is recommended to be added to the substrate for papaya seedling production, as it enhances the physical, chemical and biological characteristics of the soil, creating a favorable environment for the initial development of the plants, which results in better plant growth. The organic matter has also the important role of providing nutrients to the plants. However, there is great variation in the recommendations, and in most cases organic fertilization is associated with mineral supplementation. The objective of this research was to verify the effects of the use of corral manure, associated or not with mineral fertilization, added to the papaya seedling substrate. The experiment was deployed at the Teaching and Research Farm of the College of Engineering of Ilha Solteira, UNESP, in Ilha Solteira, state of São Paulo, Brazil. The design was set in blocks, in split plots, where papaya cultivars were the whole unit and substrates the subunit treatments. Each unit with 5 replications and the subunit composed of 10 plants each. Means were separated by Tukey test. Results indicated that: a papaya seedling production the corral manure can be used without supplementation of simple superphosphate and potassium chloride, b corral manure alone supplied nutrients N, P, K, Ca, Mg and Cu to the papaya seedlings until transplanted to the field.

  13. Quality of Guava and Papaya Fruit Pulp as Influenced by Blending Ratio and Storage Period

    Directory of Open Access Journals (Sweden)

    Prabhat K. Nema

    2011-01-01

    Full Text Available Guava and papaya are the most widely grown commercial fruits of central India. Both the fruits are nutritive and may be used for processing. The analysis of organoleptic characters (i.e., colour, flavour, texture, taste and overall acceptability and qualitative characters (i.e., TSS, pH, acidity, ascorbic acid content of guava and papaya fruits was conducted on fresh fruit, prepared pulp and mixed pulp. During the storage of fruit pulp at low temperature (61C, the decrease in overall acceptability of both the pulp was observed with increase in storage period. However, blending of both the pulp in different ratios influenced the organoleptic characters as well as the qualitative characters of the blended pulp.

  14. Empleo de un Recubrimiento Formulado con Propóleos para el Manejo Poscosecha de Frutos de Papaya (Carica papaya L. cv. Hawaiana Use of a Coating Formulated with Propolis for Postharvest Handling of Papaya (Carica papaya L. cv. Hawaiian Fruits

    Directory of Open Access Journals (Sweden)

    Elizabeth Barrera Bello

    2012-06-01

    Full Text Available Resumen. El fruto de papaya es reconocido por las propiedades nutricionales y sensoriales; no obstante, su tiempo de vida útil en poscosecha es muy corto. En los últimos años, para incrementar la vida de almacenamiento del fruto se ha implementado el uso de recubrimientos como vehículos de agentes antimicrobianos y antioxidantes, entre otros. En este trabajo se comparó el efecto de dos recubrimientos; cera comercial (control y cera comercial conteniendo un extracto etanólico de propóleos (5% p/v, sobre la vida en poscosecha de frutos de papaya (Carica papaya L. cv. Hawaiana almacenados a temperatura ambiente (28 ± 2 ºC y humedad relativa entre 65 y 70%. El efecto de los recubrimientos se determinó mediante el índice de deterioro de los frutos y el recuento microbiano (mesófilos aerobios, mohos y levaduras. Adicionalmente, se evaluaron durante 12 días las propiedades fisicoquímicas de los frutos (cambio de color, textura, pH, acidez total titulable, pérdida de peso y sólidos solubles. Los resultados mostraron que las papayas tratadas con el recubrimiento formulado con el extracto de propóleos, presentó un menor deterioro en cuanto a su apariencia y mayor inhibición del crecimiento de microorganismos durante los primeros 6 días de evaluación en comparación con los frutos control; además, no se observaron diferencias, producto de los recubrimientos, en relación a las características fisicoquímicas de los frutos.Abstract. Papaya fruit is known for the nutritional and sensory properties; however, its postharvest shelf life is very short. In recent years, to extend the storage life of this fruit, the use of coatings as a carrier of antimicrobial agents and antioxidants, has been implemented. In this work, the effect of two coatings, commercial wax (control and commercial wax containing an ethanolic extract of propolis (5% w/v on the postharvest of papaya (Carica papaya L. cv. Hawaiian was compared. The fruits were stored at room temperature (28 ± 2 ºC and a relative humidity between 65 and 70%. The effect of coatings was determined by the deterioration index of the fruit, and the microbial counts (aerobic mesophiles, molds and yeasts. Additionally, during 12 days were evaluated physicochemical properties of the fruits (color, texture, pH, titratable acidity, weight loss and soluble solids. The results showed that the papaya fruits treated with the coating containing the propolis extract presented less deterioration in their appearance and greater inhibition growth of microorganisms during the first 6 days compared with control fruits; moreover, no differences among the coatings in relation to the physicochemical characteristics of the fruit were observed.

  15. Studies on Effects of Arbuscular Mycorrhizal (Am. Fungi on Mineral Nutrition of Carica papaya L.

    Directory of Open Access Journals (Sweden)

    Sharda Waman KHADE

    2009-06-01

    Full Text Available Experiment was conducted to study the effects of arbuscular mycorrhizal fungi on mineral nutrition of Carica papaya var. Surya. The experiment comprised of un-inoculated seedlings, seedlings inoculated with Glomus intraradices Schenck & Smith, seedlings inoculated with Glomus mosseae [(Nicol. & Gerd. Gerd. & Trappe] and seedlings inoculated with mixed inoculum [Glomus intraradices (Schenck & Smith + Glomus mosseae (Nicol. & Gerd. Gerd. & Trappe]. Studies revealed that total potassium and total phosphorus content of mycorrhizal leaf petiole was higher in inoculated plants as compared to controls and varied significantly within the treatments. Glomus mosseae was the most effective species of AM fungi, in influencing mineral nutrition of papaya followed by mixed inoculum (GI +GM and Glomus intraradices respectively.

  16. Structural characterization of the papaya cysteine proteinases at low pH.

    Science.gov (United States)

    Huet, Jolle; Looze, Yvan; Bartik, Kristin; Raussens, Vincent; Wintjens, Ren; Boussard, Paule

    2006-03-10

    Current control of gastrointestinal nematodes relies primarily on the use of synthetic drugs and encounters serious problems of resistance. Oral administration of plant cysteine proteinases, known to be capable of damaging nematode cuticles, has recently been recommended to overcome these problems. This prompted us to examine if plant cysteine proteinases like the four papaya proteinases papain, caricain, chymopapain, and glycine endopeptidase that have been investigated here can survive acidic pH conditions and pepsin degradation. The four papaya proteinases have been found to undergo, at low pH, a conformational transition that instantaneously converts their native forms into molten globules that are quite unstable and rapidly degraded by pepsin. As shown by activity measurements, the denatured state of these proteinases which finally results from acid treatment is completely irreversible. It is concluded that cysteine proteinases from plant origin may require to be protected against both acid denaturation and proteolysis to be effective in the gut after oral administration. PMID:16434027

  17. Effects of mechanical wounding on Carica papaya cysteine endopeptidases accumulation and activity.

    Science.gov (United States)

    Azarkan, Mohamed; Dibiani, Rachid; Baulard, Cline; Baeyens-Volant, Danielle

    2006-05-30

    The mechanical wounding impact on the Carica papaya latex protein pattern was investigated by analyzing three latexes. A first one commercially available, a second harvested from unripe but fully grown fruits, both obtained from regularly tapped fruits. A third one was collected from similar fruits but wounded for the first time. The results demonstrated both quantitative and qualitative changes in the protein content and in the enzymatic activity. Repeated wounding results in either, accumulation or activation (or both of them) of papain, chymopapain and caricain. Furthermore, new cysteine protease activity was found to transiently accumulate in the latex collected from newly wounded fruits. The possible implication of this enzymatic material in the papaya cysteine endopeptidases pro-forms activation is discussed. PMID:16580724

  18. Effects of irradiation on the organoleptic quality and ripening process of papaya

    International Nuclear Information System (INIS)

    Papaya variety Eksotika harvested at stage 2 of maturity (green skin with a tinge of yellow) were cleaned, air dried and exposed to irradiation at lower doses i.e. 250, 500, 750, 1000 Gray and then were kept at 20 deg. C for up to 9 days or until ripe. Sensory evaluation was carried out the storage period in order to determine effects of radiation on organoleptic quality and ripening process. About 15-24 experienced panelists were involved in the sensory evaluation. It was found that effect on the organoleptic quality of fruit irradiated with dose at 1000 Gray was unacceptable. Nevertheless, the quality was very much less affected by irradiation at 750 Gray or lower. In general the irradiated papaya ripened earlier than the control where the skin colour change by turning from green to 100% yellow but gives harder texture. Freckles on skin surface during ripening were reduced by irradiation

  19. The effect of gamma radiation on the peroxides activity, colour and ascorbic acid content of papaya

    International Nuclear Information System (INIS)

    The activity of peroxidase enzyme increases during ripening with external colour used as a maturity index. An experiment indicated that 200 Krad dose level was most effective in inhibiting any increase in peroxidase activity when papaya was stored at room temperature. The doses of 25, 50 and 100 Krad level at least double the shelf life of fruit with 100 Krad was the most effective when kept in the cold. The development of yellow colour and synthesis of vitamin C is not effected. So, this preliminary study indicates that gamma radiation prolongs the shelf life of papaya when stored at cold or room temperature due to the inhibiting of the ripening process. It is also suggested that treatment of fungicides combined with gamma radiation might have an additive effect on shelf life

  20. Enzymatic wound debridement; role of papaya in the management of post cesarean gaped wounds

    International Nuclear Information System (INIS)

    Background: Enzymatic wound debridement is an emerging concept in facilitating the wound healing process. Papaya has de-sloughing, antibacterial and wound healing properties. It has been used in African countries since centuries for different medicinal pur-poses. Apart from anecdotal reports and few studies on chronic ulcers and burns, no planned studies are available to support its action in postoperative wound infection.Objectives: To compare efficacy and safety of papaya dressing with conventional wound dressing with povidone iodine in post cesarean section gaped wounds. Setting: Gynecology Unit 3, Sir Ganga Ram Hospital Lahore - Pakistan, over a period of six months(June 2012 to Nov 2012). Study Design: Randomized, quasi experimental stu-dy. Materials and Methods: The study sample included 60 patients with post cesarean section gaped wounds. The sample was divided into two groups; thirty patients as Group A or the study group received Papaya dressing and rest of thirty patients as Group B or the control group received Povidone iodine dressing. Wounds were thoroughly washed with saline and then mashed unripe papaya was spread over the whole area of wound in the study group and povidone iodine in the control group. Wounds were covered with sterile bandage for at least 48 hours in study group and 24 hours in the control group. The process was repeated till a clean base of wound with healthy granulation tis-sue was achieved suitable for secondary suture. The efficacy parameters studied were the duration of time needed to develop healthy granulation tissue and total duration of hospital stay which were compared bet-ween the two groups. Safety factors studied were the adverse effects of medications used in the study. Results: Out of 1200 cesarean sections done during study period, sixty (5%) were gaped in the post-operative period. Out of 60, 55 (90%) were emergency and only 5 (10%) were elective cesarean sections. All the sixty patients with postoperative gaped wounds were included in the study, of which 30 patients received Papaya and 30 received Povidone iodine dressing. Time required to induce healthy granulation tissue was significantly shorter in the papaya group as compared to povidone iodine group (3.4 +- 0.4 days versus 7.2 +- 0.2 days).Similarly the duration of hospital stay was also short in the study as compared to control group i.e. 11.6 +- 1.2 days and 16.8 +- 1.4 days respectively. The side effects of medications used in study (papaya and povidone iodine) like local irritation and hyper-sensitivity reactions were minor and not significally different in both groups. Conclusion: Papaya dressing is effective, safe, widely available and cost effective for wound healing in post-operative infected gaped wounds. (author)

  1. Effect of the potassium permanganate during papaya fruit ripening: Ethylene production

    Science.gov (United States)

    Corrêa, S. F.; Filho, M. B.; da Silva, M. G.; Oliveira, J. G.; Aroucha, E. M. M.; Silva, R. F.; Pereira, M. G.; Vargas, H.

    2005-06-01

    The effect of potassium permanganate (KMnO4) on the ripening process of papaya fruits by monitoring the ethylene emission rates is reported. The ethylene emission was monitored by a photoacoustic spectrometer. Two experimental conditions were applied, being one of them just putting the fruit alone inside the sampling chamber and the second, modifying the atmosphere by the presence of KMnO4. The use of the ethylene absorber reduces the autocatalytic process of ethylene during papaya fruit ripening. For 20 g of KMnO4 the maximal intensity of the ethylene emission decreases by a factor two. Using the same amount of KMnO4, a reduction of about 2.2% in the concentration of ethylene for a mixture of 1ppmv of ethylene in synthetic air was observed.

  2. GROWTH OF MACROBRACHIUM ROSENBERGII FED WITH MANGO SEED KERNEL, BANANA PEEL AND PAPAYA PEEL INCORPORATED FEEDS

    OpenAIRE

    P. Aarumugam; P. Saravana Bhavan; T. Muralisankar; Manickam, N; V. Srinevasan; Radhakrishnan, S.

    2013-01-01

    The growth promoting potential of fruits wastes, mango seed kernel, banana peel and papaya peel on the freshwater prawn, Macrobrachium rosenbergii post larvae (PL) was evaluated. Basal diet equated to 35% protein was prepared by using soybean meal, groundnut oilcake, horse gram and wheat flour. Each fruit waste powder was separately incorporated with basal diet at a proportion of 10%. Sunflower oil was used as lipid source. Egg albumin and tapioca flour were used as binding agents. Vitamin B-...

  3. Control of papaya fruits anthracnose by essential oil of Ricinus communis

    OpenAIRE

    César Luis Siqueira Júnior; Maria das Graças Machado Freire; Antônio Sérgio Nascimento Moreira; Maria Ligia Rodrigues Macedo

    2012-01-01

    The aim of this work was to investigate the potential of castor oil for the control of papaya diseases caused by the fungus Colletotrichum gloeosporioides and the bacterium Pseudomonas caricapapayae. The treatment with 1% castor oil did not significantly affect the fungal growth. The effectiveness of castor oil for the control of anthracnose was shown when 5% and 10% (v/v) were used in the assays resulting in reduced mycelial growth. Fungal sporulation was strongly inhibited at 10% (v/v) conc...

  4. Efecto del cloruro de sodio sobre el crecimiento y estado nutricional de plantas de papaya

    Directory of Open Access Journals (Sweden)

    Jorge Parés

    2013-08-01

    Full Text Available La limitada disponibilidad de agua de calidad para la agricultura de las zonas áridas y semiáridas ha propiciado el uso de agua de moderada a alta salinidad. La papaya (Carica papaya L., aunque se reporta con diferentes sensibilidades al estrés salino, es una especie de gran potencial frutícola para ser cultivada económicamente en las regiones semiáridas del estado Lara, Venezuela. El objetivo de este ensayo fue evaluar el efecto de la salinidad ocasionada por el NaCl sobre el crecimiento vegetativo y el estado nutricional de la papaya ‘Maradol’ desarrollada bajo una estructura de techo transparente. Como fuente salina se utilizó cloruro de sodio grado analítico, el cual se adicionó a través del agua de riego en cuatro tratamientos (0,001; 2,0; 4,0 y 8,0 dS·m-1 bajo un diseño completamente aleatorizado con cuatro repeticiones, hasta finalizar la fase juvenil del desarrollo. Se evaluó la longitud del tallo, biomasa seca aérea y radical, área foliar, área de sección del tallo, contenido relativo de clorofila (SPAD y el contenido de N, P, K, Ca, Mg, Mn, Zn, Cu, Fe y Na a nivel foliar. Los tratamientos afectaron el crecimiento vegetativo de las plantas durante toda la fase juvenil del desarrollo. Se observó, además, reducción del contenido de clorofila con el estrés por NaCl. Los tenores N y Na incrementaron, mientras que, los de K, Zn, Cu y Fe disminuyeron. Los niveles de P, Ca, Mg y Mn, no se afectaron. En base a los resultados obtenidos la papaya ‘Maradol’ puede ser considerada como un cultivar de moderada tolerancia al NaCl.

  5. Studies on moisture sorption isotherms for osmotically dehydrated papaya cubes and verification of selected models

    OpenAIRE

    S. K. Jain (U.S.A.); R. C. VERMA; Sharma, G P; Jain, H.K.

    2010-01-01

    The water desorption properties of osmotically dehydrated papaya cubes at various temperatures were studied by fitting experimental isotherms in Henderson, Oswin, Chen and Clayton and Kuhn equations having 2 parameters and Henderson and Oswin equations were modified to describe the temperature dependence of isotherm data. Oswin equation was useful to predict the equilibrium moisture content values for use in determining the effective moisture diffusion coefficient during subsequent air drying...

  6. Studies on moisture sorption isotherms for osmotically dehydrated papaya cubes and verification of selected models.

    Science.gov (United States)

    Jain, S K; Verma, R C; Sharma, G P; Jain, H K

    2010-06-01

    The water desorption properties of osmotically dehydrated papaya cubes at various temperatures were studied by fitting experimental isotherms in Henderson, Oswin, Chen and Clayton and Kuhn equations having 2 parameters and Henderson and Oswin equations were modified to describe the temperature dependence of isotherm data. Oswin equation was useful to predict the equilibrium moisture content values for use in determining the effective moisture diffusion coefficient during subsequent air drying process. PMID:23572650

  7. Efek Ekstrak Air Buah Pepaya (Carica papaya L. Muda terhadap Gambaran Histologi Kelenjar Mamma Mencit Laktasi

    Directory of Open Access Journals (Sweden)

    Yuktiana Kharisma

    2011-12-01

    Full Text Available Breast milk is the best food for infants. Household Health Survey in 2005 showed exclusive breastfeeding were 412% in urban and 45% in rural areas. Objective of the study was to examine the effect of unripe papaya aqueous extract (Carica papaya L. on lactating mammary glands histological appearance. The experiment was held on JulySeptember 2009 at Padjadjaran University Clinical Pharmacology Laboratory and Health Research Unit of Dr. Hasan Sadikin Hospital in JulySeptember 2009. A laboratorium experimental study conducted to 21 lactating Swiss Webster mice with 10 babies each. They were divided randomly into 3 groups (n=7. Group I was negative control. Group II: luteotropin 6 mg/30 g BW/day (per oral and group III: unripe papaya aqueous extract 20 mg/30 g BW/day (per oral. Increased milk production was measured by average lactating mammary glands alveolar amounts and diameter count. Experiment started at 4th16th lactation day. The result was analyzed using analysis of variance (ANOVA followed by Tukey test. The average of alveolar amounts of group I, II and III, were 310.5730.16, 464.4225.83, and 465.1472.41, respectively. The average lactating mamary glands alveolar diameter of group I: 296.5021.27 ?, group II: 394.5753.97 ? and group III: 384.2940.40 ?. Research showed that unripe papaya aqueous extract has better than negative control and equivalent effect with luteotropin on lactating mammary glands histological appearance

  8. Semen quality in papaya workers with long term exposure to ethylene dibromide.

    OpenAIRE

    Ratcliffe, J. M.; Schrader, S M; Steenland, K; Clapp, D E; Turner, T.; Hornung, R W

    1987-01-01

    To examine whether long term occupational exposure to ethylene dibromide (EDB) affects semen quality a cross sectional study of semen quality was conducted among 46 men employed in the papaya fumigation industry in Hawaii, with an average duration of exposure of five years and a geometric mean breathing zone exposure to airborne EDB of 88 ppb (eight hour time weighted average) and peak exposures of up to 262 ppb. The comparison group consisted of 43 unexposed men from a nearby sugar refinery....

  9. Sensory acceptance of mixed nectar of papaya, passion fruit and acerola

    Directory of Open Access Journals (Sweden)

    Matsuura Fernando César Akira Urbano

    2004-01-01

    Full Text Available Nectars are beverages formulated with the juice or pulp of one or more fruits, plus water and sugar in concentrations resulting in a "ready-to-drink" product. Recently, the market for such products has greatly expanded. Fruit mixtures present a series of advantages, such as the combination of different aromas and flavors and the sum of their nutritional components. The objective of this work was to develop a nectar based on papaya pulp and passion fruit juice, enriched with the vitamin C present in acerola pulp, optimizing the formulation using sensory consumer tests and a response surface statistical methodology. Eleven formulations were prepared using different concentrations of papaya pulp and passion fruit juice and sucrose, and maintaining the concentration of acerola pulp constant. The sensory tests were carried out with 22 non-trained panelists using a structured 9-point hedonic scale to evaluate overall acceptance. The acceptance means were submitted to regression analysis, by first calculating a polynomial quadratic equation. A predictive model was adjusted considering only those parameters where P < 0.05, and a response surface was generated. The overall acceptance of nectars of different formulations varied from 5 ("neither liked nor disliked" to more than 7 ("liked moderately", showing that some products can be considered adequate to consumers, like the nectar produced with 37.5% papaya pulp, 7.5% passion fruit juice, and 5.0% acerola pulp, added of 15% sucrose. A quadratic predictive overall acceptance model, with a regression coefficient of 0.97 was obtained. The sensory acceptance of nectars was positively affected by increases in the concentrations of papaya pulp and of sucrose. Thus, some products presented good sensory acceptance suggesting commercial potential.

  10. Phenol induced by irradiation does not impair sensory quality of fenugreek and papaya

    Science.gov (United States)

    Chatterjee, Suchandra; Variyar, Prasad S.; Sharma, Arun

    2013-11-01

    The effect of radiation processing on the sensory quality of fenugreek and papaya exposed to doses in the range of 2.5-10 kGy and 100 Gy-2.5 kGy respectively was investigated. Despite an increase in the content of phenol in the volatile oil of these food products overall sensory quality of the irradiated and control samples was not significantly affected by radiation processing.

  11. Reasoned opinion on the modification of MRLs for spirodiclofen in strawberries bananas, avocado, mango and papaya

    Directory of Open Access Journals (Sweden)

    European Food Safety Authority

    2012-07-01

    Full Text Available

    In accordance with Article 6 of Regulation (EC No 396/2005, the Netherlands, herewith referred as the evaluating Member State (EMS, received an application from Bayer S.A.S-Bayer CropScience to modify the existing MRLs for spirodiclofen in strawberries and bananas and set import tolerances for papaya, avocado and mango. The Netherlands proposed to decrease the existing MRL for strawberries from 2 mg/kg to 0.02 mg/kg and to increase the MRL for banana from the limit of quantification 0.02* mg/kg to 0.3mg/kg. In order to accommodate the import of produce, the Netherlands proposed to set the MRL for papaya, mangos and avocados at 1.0 mg/kg. The Netherlands drafted an evaluation report according to Article 8 of Regulation (EC No 396/2005, which was submitted to the European Commission and forwarded to EFSA. According to EFSA the data are sufficient to derive MRL proposals of 0.3 mg/kg for the proposed use in banana and 1.5 mg/kg to accommodate the reported use in the USA on avocado, noting that the MRL in the country of origin is set at the level of 1 mg/kg. EFSA has some reservations regarding the proposal that the residue trial results for avocado might be extrapolated to propose MRLs on papaya and mango. The intended use on strawberries is not adequately supported by residue data. Based on the risk assessment results, EFSA concludes that the proposed use of spirodiclofen on strawberry, banana, avocado, mango and papaya will not result in consumer exposure exceeding the toxicological reference value and therefore is unlikely to pose a consumer health risk.

  12. Prospects and problems of irradiating papaya. A case study in Hawaii

    International Nuclear Information System (INIS)

    The United States Environmental Protection Agency's (EPA's) ban of ethylene dibromide (EDB) as a fruit fumigant has forced Hawaii's papaya industry to search for alternative treatments. Among various alternatives considered, irradiation process is the most efficacious because fruits can be sorted, packaged, chilled and conveyed to an irradiator for low-dose treatments (0.26 kGy for disinfestation) before shipment to export markets. The papaya industry in Hawaii, however, has not assigned a high priority to the irradiation process. Instead, the industry opted for the double-dip hot water treatment which was rushed to become an USDA-approved procedure shortly before 1 September 1984. Three major concerns expressed by the papaya industry about the irradiation process as a replacement for chemical fumigation are: (1) Capital investment; (2) Logistics of irradiation processing and fruit transport; and (3) Consumer acceptance. The outlook for radiation disinfestation of Hawaii-grown papaya is quite good in spite of these concerns expressed by the industry. Some packers are beginning to feel that there are more advantages and benefits in adopting this process than the disadvantage of negative publicity about nuclear technology. With the availability of irradiation, the fruit and vegetable industry in Hawaii could be expanded by increased production and sales of new or existing crops. A worthwhile task ahead would be for the food industry, government agencies and researchers to join forces in conducting an effective consumer education programme by assuring the public that irradiated foods are safe. Concurrently, certain segments of the food industry should consider and prepare for the processing and marketing of irradiated foods. (author)

  13. Efficiency of a yeast-based formulation for the biocontrol of postharvest anthracnose of papayas

    OpenAIRE

    Jaqueline Rabelo de Lima; Francisco Marto Pinto Viana; Francisco Aldiel Lima; Vanessa Pieniz; Luciana Rocha Barros Gonçalves

    2014-01-01

    To identify formulations of biological agents that enable survival, stability and a good surface distribution of the antagonistic agent, studies that test different application vehicles are necessary. The efficiency of two killer yeasts, Wickerhamomyces anomalus (strain 422) and Meyerozyma guilliermondii (strain 443), associated with five different application vehicles, was assessed for the protection of postharvest papayas. In this study, after 90 days of incubation at 4ºC, W. anomalus (stra...

  14. Dynamic transposable element accumulation in the nascent sex chromosomes of papaya

    OpenAIRE

    VanBuren, Robert; Ming, Ray

    2013-01-01

    From their inception, Y chromosomes in plants and animals are subjected to the powerful effects of Mllers ratchet, a process spurred by suppression of recombination that results in a rapid accumulation of mutations and repetitive elements. These mutations eventually lead to gene loss and degeneration of the Y chromosome. Y chromosomes in mammals are ancient, whereas most sex chromosomes in plants and many in insects and fish evolved recently. Sex type in papaya is controlled by a pair of na...

  15. Color index and correlation with physical and chemical parameters of guava, mango and papaya

    Directory of Open Access Journals (Sweden)

    Jedman Dantas Motta

    2015-03-01

    Full Text Available It was used a low cost colorimeter determine the color in agricultural products. Color analysis on peel and pulps of guava, papaya and mango were made. The values found in the L*a*b* color spaces were used to calculate color indexes that were related to the soluble solids content, pH and the fruit consistence during the maturation. The results obtained permit to conclude that it is possible to estimate the ripening of papaya over the color peel, having found an accurate relation between peel color index and soluble solids content expressed in terms of the third degree equation. Nevertheless for the guava and mango there is not a relation between the peel color indexes and soluble solids content. There is not a relation between color indexes of the guava, papaya and mango pulps with soluble solids content. For all fruits studied there was not relation between color indexes of the peel or pulp with pH.

  16. Antifungal Activity in Ethanolic Extracts of Carica papaya L. cv. Maradol Leaves and Seeds.

    Science.gov (United States)

    Chvez-Quintal, Pedro; Gonzlez-Flores, Tania; Rodrguez-Buenfil, Ingrid; Gallegos-Tintor, Santiago

    2011-01-01

    Bioactive compounds from vegetal sources are a potential source of natural antifungic. An ethanol extraction was used to obtain bioactive compounds from Carica papaya L. cv. Maradol leaves and seeds of discarded ripe and unripe fruit. Both, extraction time and the papaya tissue flour:organic solvent ratio significantly affected yield, with the longest time and highest flour:solvent ratio producing the highest yield. The effect of time on extraction efficiency was confirmed by qualitative identification of the compounds present in the lowest and highest yield extracts. Analysis of the leaf extract with phytochemical tests showed the presence of alkaloids, flavonoids and terpenes. Antifungal effectiveness was determined by challenging the extracts (LE, SRE, SUE) from the best extraction treatment against three phytopathogenic fungi: Rhizopus stolonifer, Fusarium spp. and Colletotrichum gloeosporioides. The leaf extract exhibited the broadest action spectrum. The MIC(50) for the leaf extract was 0.625mgml(-1) for Fusarium spp. and >10mgml(-1) for C. gloeosporioides, both equal to approximately 20% mycelial growth inhibition. Ethanolic extracts from Carica papaya L. cv. Maradol leaves are a potential source of secondary metabolites with antifungal properties. PMID:22282629

  17. LEAF GAS EXCHANGE CHARACTERISTICS OF FOUR PAPAYA GENOTYPES DURING DIFFERENT STAGES OF DEVELOPMENT

    Directory of Open Access Journals (Sweden)

    CAMPOSTRINI ELIEMAR

    2001-01-01

    Full Text Available In this research, was used four papaya (Carica papaya L. genotypes: three from the 'Solo ( Sunrise Solo TJ, Sunrise Solo 72/12 and Baixinho de Santa Amália group and one from the 'Formosa' group (Know-You 01. They were grown in plastic pots containing a sandy-clay-loam soil subjected to pH correction and fertilization, under greenhouse conditions. Throughout the experimental period plants were subjected to periodic irrigation to maintain the soil humitidy around field capacity. The experiment was conducted 73 days after sowing. In all genotypes, leaf gas exchange characteristics were determined. The net photosynthetic rate (A, mumol m-2 s-1 , stomatal conductance (g s mol m-2 s-1, leaf temperature (T I, 0C and intercellular carbon dioxide concentration (ci, muL L-1 on the 4th, 5th, 6th, 7th, 8th and 9th leaves from the plant apex were determined. No significant differences were observed for A, g s, c i, or Tl either among the leaves sampled from any of the genotypes. A was positively correlated with g s and in the other hand T I and g s were negatively correlated. The results suggest that, for 73 DAP, all the sampled papaya leaves functioned as sources of organs.

  18. Sequencing papaya X and Yh chromosomes reveals molecular basis of incipient sex chromosome evolution.

    Science.gov (United States)

    Wang, Jianping; Na, Jong-Kuk; Yu, Qingyi; Gschwend, Andrea R; Han, Jennifer; Zeng, Fanchang; Aryal, Rishi; VanBuren, Robert; Murray, Jan E; Zhang, Wenli; Navajas-Pérez, Rafael; Feltus, F Alex; Lemke, Cornelia; Tong, Eric J; Chen, Cuixia; Wai, Ching Man; Singh, Ratnesh; Wang, Ming-Li; Min, Xiang Jia; Alam, Maqsudul; Charlesworth, Deborah; Moore, Paul H; Jiang, Jiming; Paterson, Andrew H; Ming, Ray

    2012-08-21

    Sex determination in papaya is controlled by a recently evolved XY chromosome pair, with two slightly different Y chromosomes controlling the development of males (Y) and hermaphrodites (Y(h)). To study the events of early sex chromosome evolution, we sequenced the hermaphrodite-specific region of the Y(h) chromosome (HSY) and its X counterpart, yielding an 8.1-megabase (Mb) HSY pseudomolecule, and a 3.5-Mb sequence for the corresponding X region. The HSY is larger than the X region, mostly due to retrotransposon insertions. The papaya HSY differs from the X region by two large-scale inversions, the first of which likely caused the recombination suppression between the X and Y(h) chromosomes, followed by numerous additional chromosomal rearrangements. Altogether, including the X and/or HSY regions, 124 transcription units were annotated, including 50 functional pairs present in both the X and HSY. Ten HSY genes had functional homologs elsewhere in the papaya autosomal regions, suggesting movement of genes onto the HSY, whereas the X region had none. Sequence divergence between 70 transcripts shared by the X and HSY revealed two evolutionary strata in the X chromosome, corresponding to the two inversions on the HSY, the older of which evolved about 7.0 million years ago. Gene content differences between the HSY and X are greatest in the older stratum, whereas the gene content and order of the collinear regions are identical. Our findings support theoretical models of early sex chromosome evolution. PMID:22869747

  19. Efficiency of a yeast-based formulation for the biocontrol of postharvest anthracnose of papayas

    Directory of Open Access Journals (Sweden)

    Jaqueline Rabelo de Lima

    2014-09-01

    Full Text Available To identify formulations of biological agents that enable survival, stability and a good surface distribution of the antagonistic agent, studies that test different application vehicles are necessary. The efficiency of two killer yeasts, Wickerhamomyces anomalus (strain 422 and Meyerozyma guilliermondii (strain 443, associated with five different application vehicles, was assessed for the protection of postharvest papayas. In this study, after 90 days of incubation at 4ºC, W. anomalus (strain 422 and M. guilliermondii (strain 443 were viable with all application vehicles tested. Fruits treated with different formulations (yeasts + application vehicles had a decreased severity of disease (by at least 30% compared with untreated fruits. The treatment with W. anomalus (strain 422 + 2% starch lowered disease occurrence by 48.3%. The most efficient treatments using M. guilliermondii (strain 443 were those with 2% gelatin or 2% liquid carnauba wax, both of which reduced anthracnose by 50% in postharvest papayas. Electron micrographs of the surface tissues of the treated fruits showed that all application vehicles provided excellent adhesion of the yeast to the surface. Formulations based on starch (2%, gelatin (2% and carnauba wax (2% were the most efficient at controlling fungal diseases in postharvest papayas.

  20. Gamma irradiation as a quarantine treatment for carambola, papaya and mango

    International Nuclear Information System (INIS)

    Preliminary experiments carried out on the effects of irradiation on carambola (Averrhoa carambola L.), papaya (Carica papaya L.) and mango (Mangifera indica L.) with regard to fruit fly treatment, fruit injury and the physicochemical and organoleptic properties showed that irradiation can be successfully developed and should be investigated further as a quarantine treatment for these fruits. Emergence of normal adult fruit flies of the Dacus dorsalis complex did not occur when infested carambolas were treated at doses as low as 100 Gy. Carambola showed external symptoms of injury at irradiation doses in excess of 200 Gy. There appeared to be some reduction in sugar content at doses exceeding 100 Gy. Papaya, cv. ''Eksotika'', tolerated irradiation up to 300 Gy. Irradiation at this dose did not alter the ripening behaviour, nor did it cause any injury or alter the organoleptic properties of the fruit. An additional benefit was that doses above 250 Gy significantly reduced freckling of the fruit and enhanced its cosmetic value. ''Eksotika'' is an ideal candidate for quarantine treatment using gamma irradiation. Mango, cv. ''Harumanis'', tolerated irradiation fairly well. Exposure of fruit to doses of up to 750 Gy did not produce significant injury. (author). 13 refs, 2 figs, 17 tabs

  1. Consideraciones sobre la utilización de diferentes densidades en el cultivo de papaya (Carica papaya, L. "Baixinho de Santa Amalia" en islas canarias

    Directory of Open Access Journals (Sweden)

    Pastor Mª Cristina Rodriguez

    2002-01-01

    Full Text Available El cultivo de papaya en Islas Canarias se ha extendido en los últimos años bajo condiciones de invernadero. La utilización de cultivares de papaya tipo "Baixinho de Santa Amalia" (mutante natural del cultivar "Sunrise" de porte bajo, emisión de la flor a corta altura y precocidad en la floración, resultan de gran interés sobre todo en esta clase de medios. Estas características fenológicas hacen posible el manejo del cultivo a mayores densidades que las empleadas con otros cultivares. Por lo tanto, se ha planteado este trabajo cuyo objetivo principal es determinar cual es el marco de plantación óptimo, que permita obtener mayores rendimientos sin depreciar la calidad del fruto. Para ello, se ha evaluado la producción tanto de las plantas hermafroditas como de las plantas femeninas durante dos ciclos de cultivo, así como las características organolépticas, grado de carpeloidía y deformación de los frutos. Los resultados indican que la densidad mayor, proporciona mejor comportamiento de las plantas así como mayor producción de frutos y menor porcentaje de fruta desechable.

  2. Gastroprotective and Anti-ulcer activity of Aloe vera juice, Papaya fruit juice and Aloe vera and Papaya fruit combined juice in Ethanol induced Ulcerated Rats

    Directory of Open Access Journals (Sweden)

    S. Gopinathan

    2013-12-01

    Full Text Available Peptic ulcer is the most prevalent gastrointestinal disease. Even though a wide range of drugs are available for the treatment of peptic ulcer, but many of these do not fulfill all the requirements and have side effects. These factors have attracted researchers to investigate the natural products which have more efficacy, less side effects and less expensive for the treatment of peptic ulcer disease. In the present study the anti ulcer activity of (1 Aloe vera juice, (2 papaya fruit juice (3 Aloe vera and papaya fruit combined juice were investigated in the ethanol induced ulcerated rats. The administration of plant juices decreased the offensive factors like ulcer index and acid secretion and also reduced the amount of protein and carbohydrates in the stomach fluid. Further, plant juices increased the defensive factors like activity of oxidative enzymes such as superoxide dismutase and reduced glutathione. Activities of alkaline phosphatase and lipid peroxide were higher in the diseased condition and same were reduced after the treatment with plant juices. Content of haemoglobin and RBC and WBC counts were brought back to normalcy after the treatment with plant juices. The efficacy of plant juices was comparable with the reference drug- Ranitidine. The results of the present study reveal that the plant juices are having efficiency in the gastroprotective activity. It is recommended that the above said plant juices can be further studied for their anti ulcer efficacy in human subjects.

  3. Morfología de la flor y de la semilla de papaya (Carica papaya L.: variedad Maradol e híbrido Tainung-1

    Directory of Open Access Journals (Sweden)

    Gil Arlette Ivonne

    2005-12-01

    Full Text Available

    Con el objetivo de analizar la morfología de la flor y de la semilla de papaya (Carica papaya L. de la variedad ‘Maradol’ y el híbrido ‘Tainung-1’, se recolectó el material vegetal en dos plantaciones y se llevaron al laboratorio de Fisiología de Cultivos, Facultad de Agronomía, Universidad Nacional de Colombia, Bogotá, con el fin de realizar las descripciones correspondientes de los tres tipos de flores (femeninas, hermafroditas y estaminadas y de la semilla, sus características externas (forma, tamaño, hilo, micrópilo, funículo, rafe y cubierta seminal e internas (cubierta seminal, endospermo y embrión. Se concluyó principalmente que a nivel morfológico las flores y semillas de los dos materiales evaluados mostraron características similares, por tanto, las descripciones dadas en este trabajo son generales para ambos.

  4. La solidez de la economa chilena a la luz de las reformas estructurales: 1970-1997

    Directory of Open Access Journals (Sweden)

    ngeles SNCHEZ DEZ

    2009-11-01

    Full Text Available RESUMEN: En el presente artculo se recogen las principales reformas estructurales que han tenido lugar en Chile desde 1970 y que constituyen la base de la solidez actual de dicha economa latinoamericana. Se propone una divisin en perodos en la que se distingue la poca del gobierno de la Unidad Popular, el gobierno de la Junta Militar y la transicin a la democracia para evaluar los resultados econmicos que se registraron a la luz de las distintas estrategias de desarrollo. Las principales reformas que se dieron en el periodo 1970-1973 bajo la "va chilena hacia el socialismo" fueron la creacin del rea de Propiedad social, la profundizacin de la Reforma Agraria y la nacionalizacin de la Gran Minera Durante el monetarismo ortodoxo (1974-1982 destacan como principales reformas la liberalizacin y desregulacin de los mercados, la l a ronda de privatizaciones, la apertura comercial, la liberalizacin financiera y las "siete modernizaciones", todas ellas respaldadas por la ortodoxia de la denominada Escuela de Chicago. Tras la profunda crisis de 1982 que afect a todo el continente latinoamericano fue necesario replantear el modelo y se introdujeron distintas medidas de carcter neoliberal que consiguieron que fuera posible aprovechar los beneficios de las reformas realizadas con anterioridad. La transicin a la democracia constituye un reto para la sociedad chilena, en tanto que contando con las bases de una economa slida deben incorporarse la visin social y poltica para que el crecimiento se convierta en desarrollo. En este sentido toma importancia la ltima estrategia de la CEPAL de "Transformacin productiva con equidad".ABSTRACT: In this paper, the main structural reforms are searched, which happened between 1970 and 1995. These are the base of the actual economic framework. The different considered periods are: the "Unidad Popular" government, the military government and the democratic transition. Finally, we must evaluate the economic results that are dependent of the implementation of the reforms and of the different development strategies. The main reforms that occurred in 1970-1973 with the "va chilena hacia el socialismo" were: The creation of "area de propiedad social" social property area-, the agrarian reform and the nationalisation of the most important mining companies. In the orthodox monetarism (1974-1982, the liheralisation and deregulation of the markets, the 1" Stage/Round of privatisation, the trade and the financial liberation and the seven modernizations were the most outstanding reforms, which were supported by the Chicago School. The heavy crisis which affected Latin- American countries and also Chile, caused the reforms of monetarism made possible the improvement of the effect of the reforms of the Chicago Boys. Finally the transition to democracy was am important challenge to Chilean society because Chile had the bases of a solid economy, but it was necessary to attend social and political issues to change growth into development. For this reason the last strategy of the ECLAC "Transformacin productiva con equidad" is so important.

  5. A physical map of the papaya genome with integrated genetic map and genome sequence

    Directory of Open Access Journals (Sweden)

    Luo Ming-Cheng

    2009-08-01

    Full Text Available Abstract Background Papaya is a major fruit crop in tropical and subtropical regions worldwide and has primitive sex chromosomes controlling sex determination in this trioecious species. The papaya genome was recently sequenced because of its agricultural importance, unique biological features, and successful application of transgenic papaya for resistance to papaya ringspot virus. As a part of the genome sequencing project, we constructed a BAC-based physical map using a high information-content fingerprinting approach to assist whole genome shotgun sequence assembly. Results The physical map consists of 963 contigs, representing 9.4 genome equivalents, and was integrated with the genetic map and genome sequence using BAC end sequences and a sequence-tagged high-density genetic map. The estimated genome coverage of the physical map is about 95.8%, while 72.4% of the genome was aligned to the genetic map. A total of 1,181 high quality overgo (overlapping oligonucleotide probes representing conserved sequences in Arabidopsis and genetically mapped loci in Brassica were anchored on the physical map, which provides a foundation for comparative genomics in the Brassicales. The integrated genetic and physical map aligned with the genome sequence revealed recombination hotspots as well as regions suppressed for recombination across the genome, particularly on the recently evolved sex chromosomes. Suppression of recombination spread to the adjacent region of the male specific region of the Y chromosome (MSY, and recombination rates were recovered gradually and then exceeded the genome average. Recombination hotspots were observed at about 10 Mb away on both sides of the MSY, showing 7-fold increase compared with the genome wide average, demonstrating the dynamics of recombination of the sex chromosomes. Conclusion A BAC-based physical map of papaya was constructed and integrated with the genetic map and genome sequence. The integrated map facilitated the draft genome assembly, and is a valuable resource for comparative genomics and map-based cloning of agronomically and economically important genes and for sex chromosome research.

  6. Repeated Dose 28-Days Oral Toxicity Study of Carica papaya L. Leaf Extract in Sprague Dawley Rats

    Directory of Open Access Journals (Sweden)

    Hussin Muhammad

    2012-04-01

    Full Text Available Carica papaya L. leaves have been used in ethnomedicine for the treatment of fevers and cancers. Despite its benefits, very few studies on their potential toxicity have been described. The aim of the present study was to characterize the chemical composition of the leaf extract from ‘Sekaki’ C. papaya cultivar by UPLC-TripleTOF-ESI-MS and to investigate the sub-acute oral toxicity in Sprague Dawley rats at doses of 0.01, 0.14 and 2 g/kg by examining the general behavior, clinical signs, hematological parameters, serum biochemistry and histopathology changes. A total of twelve compounds consisting of one piperidine alkaloid, two organic acids, six malic acid derivatives, and four flavonol glycosides were characterized or tentatively identified in the C. papaya leaf extract. In the sub-acute study, the C. papaya extract did not cause mortality nor were treatment-related changes in body weight, food intake, water level, and hematological parameters observed between treatment and control groups. Some biochemical parameters such as the total protein, HDL-cholesterol, AST, ALT and ALP were elevated in a non-dose dependent manner. Histopathological examination of all organs including liver did not reveal morphological alteration. Other parameters showed non-significant differences between treatment and control groups. The present results suggest that C. papaya leaf extract at a dose up to fourteen times the levels employed in practical use in traditional medicine in Malaysia could be considered safe as a medicinal agent.

  7. Study about papaya (Carica papaya L.) conservation submitted to a combined treatment of irradiation and an edible film of quitosan; Estudo do tratamento combinado de radiacao ionizante e cobertura de quitosana em mamao papaia (Carica papaya L.)

    Energy Technology Data Exchange (ETDEWEB)

    Camargo, Rita Junqueira de

    2004-07-01

    In this research, on a first stage the effect of two treatments were studied: thermal, and a chitosan edible coating, combined with four different irradiation doses: 0,00 kGy, 0,50 kGy, 0,75 kGy and 1,00 kGy for effects in the ripening of papaya (Carica Papaya) cv. Golden. To evaluate the influence of these two factors in the physical-chemical characteristics of the fruit during ripening, analysis on color, both skin and pulp, firmness, soluble solids, pH and titrable acidity were performed. The incidence of fungi in fruit skin was also analyzed. The fruits were first maintained at 10 deg C chamber for fifteen days to simulate maritime transportation to the importer country and then transferred to a 25 deg C chamber aiming simulation of the commercialization conditions. From this first step, the conclusion was that chitosan in the concentration of 2% was not able to contain the fungi development and that it also influenced in a significant form the variable pH. A more expressive result was obtained for irradiation doses of 0,75 kGy and 1,00 kGy, as it was observed in the beginning the acceleration in ripening through the variables skin color and penetration energy of the fruit pulp. When storage temperature was changed to 25 deg C this situation inverted for these same variables and a two day delay was observed on the development of yellow color on the fruit skin and loss of pulp firmness. The variables titrable acidity, soluble solids and pulp color were not influenced by treatment or by irradiation. On a second stage, the fruits, in the same condition of the previous stage, were analyzed in relation of their mass and ripening stage using a ripening scale provided by the producer of the fruits for this experiment, as to evaluate the gradual development of fruit ripening according to the various treatments (chitosan 3%, thermal or control) and irradiation dose (0,00 kGy and 0,75 kGy). Confirming the results obtained in the first stage, the irradiation was able to delay ripening in one day and a half approximately. The chitosan in these new conditions of test propitiated fungi development and the fruits coated with chitosan did not ripe in the way it was seen to come. A last stage was done to verify consumer behavior in respect to an irradiated papaya. By three different sensorial tests it was verified if provers were able to perceive significant difference between irradiated and non irradiated samples, what was the acceptance of a irradiated sample and the intention of purchase of a irradiated and a non irradiated papaya. Conclusion from these tests was that the difference established in the triangular test was not enough to establish significant difference between the acceptances of the two samples in the four parameters tested: appearance, texture, aroma and flavor. For the purchase intention it was verified that the irradiation is not a barrier for the consumer. (author)

  8. Control of egg hatch ability and adult emergence of three fruit fly species in papayas by gamma irradiation

    International Nuclear Information System (INIS)

    The effects of gamma radiation on the oriental fruit fly, Bactrocera Dorsalis (Hendel), melon fly, Bactrocera Cucurbitae (Coquilett), and Mediterranean fruit fly, Ceratitis Capitata (Weidemann) were studied. Melon fly was determined to be the most susceptible of the three species. A dosage of 550 Gy rendered the eggs 100% sterile when irradiated in papayas at 4-6 hours before hatching. Oriental and mediterranean fruit flies were found to be more resistant, requiring doses of 750 and 850 Gy, respectively. A dose of only 100 Gy was needed to inhibit adult eclosion when the three species were treated at third instar larvae. Warm water treatment at 490C for 20 minutes was found sufficient in preventing the hatching of any egg in the infested papaya fruits. However, since eggs may hatch before the warm-water treatment can be applied, a combination of irradiation treatment using 100 Gy is recommended for disinfestation of papaya fruits. (author). 17 refs.; 3 tabs

  9. La Asociacin Chilena de Editores de Revistas Biomdicas / The Chilean Association of Biomedical Journal Editors

    Scientific Electronic Library Online (English)

    Humberto, Reyes B.

    2001-01-01

    Full Text Available [...] Abstract in english On September 29th, 2000, The Chilean Association of Biomedical Journal Editors was founded, sponsored by the "Comisin Nacional de Investigacin Cientfica y Tecnolgica (CONICYT)" (the Governmental Agency promoting and funding scientific research and technological development in Chile) and the "Soc [...] iedad Mdica de Santiago" (Chilean Society of Internal Medicine). The Association adopted the goals of the World Association of Medical Editors (WAME) and therefore it will foster "cooperation and communication among Editors of Chilean biomedical journals; to improve editorial standards, to promote professionalism in medical editing through education, self-criticism and self-regulation; and to encourage research on the principles and practice of medical editing". Twenty nine journals covering a closely similar number of different biomedical sciences, medical specialties, veterinary, dentistry and nursing, became Founding Members of the Association. A Governing Board was elected: President: Humberto Reyes, M.D. (Editor, Revista Mdica de Chile); Vice-President: Mariano del Sol, M.D. (Editor, Revista Chilena de Anatoma); Secretary: Anna Mara Prat (CONICYT); Councilors: Manuel Krauskopff, Ph.D. (Editor, Biological Research) and Maritza Rahal, M.D. (Editor, Revista de Otorrinolaringologa y Ciruga de Cabeza y Cuello). The Association will organize a Symposium on Biomedical Journal Editing and will spread information stimulating Chilean biomedical journals to become indexed in international databases and in SciELO-Chile, the main Chilean scientific website (www.scielo.cl) (Rev Md Chile 2001;129: 95-98)

  10. LOS DERECHOS SOCIALES DE PRESTACIÓN EN LA JURISPRUDENCIA CHILENA

    Directory of Open Access Journals (Sweden)

    José Ignacio Martínez Estay

    2010-01-01

    Full Text Available Parte importante de los derechos sociales consisten en prestaciones. Desde el punto de vista jurídico ello parece ser una limitante para su garantía y satisfacción, pues dependen de los recursos económicos de que disponga el Estado. Por eso, lo usual es que si se constitucionalizan no llevan aparejada una garantía jurisdiccional. Así ocurre en la Constitución chilena. Dicha limitación se ve ratificada por la jurisprudencia de la Corte Suprema, que reiteradamente ha negado lugar a recursos de protección en que se intentaba la tutela de derechos sociales consistentes en prestaciones, vinculándolos a algún derecho protegido jurisdiccionalmente. Pero la reciente jurisprudencia del Tribunal Constitucional respecto del derecho a la protección de la salud y del derecho a la seguridad social contradice lo anterior, atribuyéndole a los derechos sociales consistentes en prestaciones el mismo valor que el de los demás derechos y libertades.

  11. La Asociacin Chilena de Editores de Revistas Biomdicas The Chilean Association of Biomedical Journal Editors

    Directory of Open Access Journals (Sweden)

    Humberto Reyes B

    2001-01-01

    Full Text Available On September 29th, 2000, The Chilean Association of Biomedical Journal Editors was founded, sponsored by the "Comisin Nacional de Investigacin Cientfica y Tecnolgica (CONICYT" (the Governmental Agency promoting and funding scientific research and technological development in Chile and the "Sociedad Mdica de Santiago" (Chilean Society of Internal Medicine. The Association adopted the goals of the World Association of Medical Editors (WAME and therefore it will foster "cooperation and communication among Editors of Chilean biomedical journals; to improve editorial standards, to promote professionalism in medical editing through education, self-criticism and self-regulation; and to encourage research on the principles and practice of medical editing". Twenty nine journals covering a closely similar number of different biomedical sciences, medical specialties, veterinary, dentistry and nursing, became Founding Members of the Association. A Governing Board was elected: President: Humberto Reyes, M.D. (Editor, Revista Mdica de Chile; Vice-President: Mariano del Sol, M.D. (Editor, Revista Chilena de Anatoma; Secretary: Anna Mara Prat (CONICYT; Councilors: Manuel Krauskopff, Ph.D. (Editor, Biological Research and Maritza Rahal, M.D. (Editor, Revista de Otorrinolaringologa y Ciruga de Cabeza y Cuello. The Association will organize a Symposium on Biomedical Journal Editing and will spread information stimulating Chilean biomedical journals to become indexed in international databases and in SciELO-Chile, the main Chilean scientific website (www.scielo.cl (Rev Md Chile 2001;129: 95-98

  12. Equilibrio trabajo-familia: corresponsabilidad familiar y autoeficacia parental en trabajadores de una empresa chilena

    Directory of Open Access Journals (Sweden)

    Alejandra Ortega Farías

    2013-01-01

    Full Text Available En el artículo se analizan las diferencias entre equilibrio trabajo-familia, corresponsabilidad y autoeficacia parental en trabajadores de una empresa chilena. A 224 trabajadores les fueron aplicados tres instrumentos: el cuestionario de interacción trabajofamilia, el cuestionario de participación en el trabajo familiar y la escala de autoeficacia parental. Se observa que no existe diferencia en el nivel de equilibrio trabajo-familia entre hombres y mujeres (t [222] = -0,83, p > 0,05. En corresponsabilidad se evidencian diferencias estadísticamente significativas (t [220,28] = -7,27, p < 0,05, indicando que las mujeres realizan más tareas en el hogar, a pesar de su jornada laboral. En relación con la autoeficacia parental existen diferencias entre hombres y mujeres (t [215] = -8,76, p <0,05, indicando que las mujeres se perciben a sí mismas como mejores madres.

  13. Regiones fronterizas y flujos culturales: La peruanidad en una región chilena

    Directory of Open Access Journals (Sweden)

    Juan Podestá Arzubiaga

    2011-01-01

    Full Text Available El artículo discute las dinámicas culturales que articulan la zona fronteriza del norte de Chile (Arica y sur peruano (Tacna. El argumento central es que se observa un creciente proceso de influencia de la cultura peruana en la ciudad de Arica, fenómeno que denominamos "la peruanidad de una región chilena". Los factores que influyen tienen que ver con el impacto regional de la globalización; la prolongada crisis económica de Arica; el acelerado crecimiento económico del sur de Perú; el agotamiento del clásico enfoque de la geo-política, y la influencia de los nuevos programas educacionales.This article discusses the cultural dynamics that shape the border area in Northern Chile (Arica and Southern Peru (Tacna. The main argument is the emergence of a growing influence of Peruvian culture in the city of Arica, which we label as ¨Peruvinization of a Chilean Region¨. The factors that influence this process have to do with the regional impact of globalization, the prolonged economic crisis in Arica, the accelerated economic growth of Southern Peru, the outdating of the classical geopolitical approach, and the influence of new educational programs.

  14. Verbo de concordancia en la lengua de señas chilena

    Directory of Open Access Journals (Sweden)

    Dora Adamo Quintela

    1999-09-01

    Full Text Available El presente trabajo trata sobre una clase verbo de la lengua de señas chilena, el verbo de concordancia. A través de su descripción se da cuenta de los diversos mecanismos utilizados por el sistema de la lengua de señas para establecer la concordancia entre el sujeto y el objeto, que funcionan en la oración mediante el proceso de inflexión gramatical. Una característica relevante de la LSCh consiste en la utilización del espacio para producir modificaciones gramaticales regulares, entendidas como procesos de inflexión, a través de la co-ocurrencia de parámetros gestual-visuales, que permiten una comunicación eficiente entre sus usuarios. (This article focuses on a particular type of verb used in Chilean Sign Language: the verb of agreement. Through its description, different mechanisms used to show subject-object accord operating through inflection are presented. A relevant feature of Chilean Sign Language (LSCh, is to use space to mark regular grammatical modifications, understood as processes of inflection through the co-occurrence of visual-kinetic parameters that allow its user an efficient communication.

  15. Prevalencia de vaginosis bacteriana en trabajadoras sexuales chilenas / Prevalence of bacterial vaginosis in Chilean sex workers

    Scientific Electronic Library Online (English)

    Gloria, Venegas; Gioconda, Boggiano; Erica, Castro.

    2011-07-01

    Full Text Available OBJETIVO: Determinar la prevalencia de vaginosis bacteriana (VB) en trabajadoras sexuales chilenas y relacionar los hallazgos con variables sociodemogrficas, sexuales y clnicas. MTODO: Se estudi una muestra de 379 trabajadoras sexuales que asistan para control a Unidades de Atencin y Control d [...] e Salud Sexual de Chile. A todas se las entrevist para obtener antecedentes sociodemogrficos y sexuales, se les realiz evaluacin clnica que incluy caractersticas del flujo vaginal, pH y prueba de aminas, y se les tom una muestra vaginal para tincin de Gram. Para el diagnstico de VB se emple el criterio de Nugent. Los datos fueron analizados con EPI-INFO 3.4.1 y BioStat, utilizndose un grado de significacin de P Abstract in english OBJECTIVE: To determine the prevalence of bacterial vaginosis (BV) in Chilean sex workers and relate the findings to sociodemographic, sexual, and clinical variables. METHODS: A sample of 379 sex workers seen in Chilean Sexual Health Monitoring and Care Units for check-ups was studied. All of them w [...] ere interviewed to obtain their sociodemographic and sexual history. A clinical examination was performed that included the characteristics of vaginal discharge, pH, and amine test. A vaginal sample was taken for Gram stain. The Nugent criteria were used for the diagnosis of BV. The data was analyzed with EPI-INFO 3.4.1 and BioStat, using a degree of significance of P

  16. Inhibition of Platelet Aggregation by the Leaf Extract of Carica papaya During Dengue Infection: An In Vitro Study.

    Science.gov (United States)

    Chinnappan, Shobia; Shettikothanuru Ramachandrappa, Vijayakumar; Tamilarasu, Kadhiravan; Krishnan, Uma Maheswari; Balakrishna Pillai, Agiesh Kumar; Rajendiran, Soundravally

    2016-04-01

    Dengue cases were reported to undergo platelet activation and thrombocytopenia by a poorly understood mechanism. Recent studies suggested that Carica papaya leaf extract could recover the platelet count in dengue cases. However, no studies have attempted to unravel the mechanism of the plant extract in platelet recovery. Since there are no available drugs to treat dengue and considering the significance of C. papaya in dengue treatment, the current study aimed to evaluate two research questions: First one is to study if the C. papaya leaf extract exerts its action directly on platelets and second one is to understand if the extract can specifically inhibit the platelet aggregation during dengue viral infection. Sixty subjects with dengue positive and 60 healthy subjects were recruited in the study. Platelet-rich plasma (PRP) and platelet-poor plasma were prepared from both the dengue-infected and healthy control blood samples. Effect of the leaf extract obtained from C. papaya leaves was assessed on plasma obtained as well as platelets collected from both healthy and dengue-infected individuals. Platelet aggregation was significantly reduced when leaf extract preincubated with dengue plasma was added into control PRP, whereas no change in aggregation when leaf extract incubated-control plasma was added into control PRP. Upon direct addition of C. papaya leaf extract, both dengue PRP and control PRP showed a significant reduction in platelet aggregation. Within the dengue group, PRP from severe and nonsevere cases showed a significant decrease in aggregation without any difference between them. From the study, it is evident that C. papaya leaf extract can directly act on platelet. The present study, the first of its kind, found that the leaf extract possesses a dengue-specific neutralizing effect on dengue viral-infected plasma that may exert a protective role on platelets. PMID:26910599

  17. Repeated Dose 28-Days Oral Toxicity Study of Carica papaya L. Leaf Extract in Sprague Dawley Rats

    OpenAIRE

    Hussin Muhammad; Ibrahim Jantan; Noordini Abdullah; Raja Hazlini Raja Semail; Badrul Amini Rashid; Siti Zaleha Halim; Noor Rain Abdullah; Adlin Afzan; Zakiah Ismail

    2012-01-01

    Carica papaya L. leaves have been used in ethnomedicine for the treatment of fevers and cancers. Despite its benefits, very few studies on their potential toxicity have been described. The aim of the present study was to characterize the chemical composition of the leaf extract from Sekaki C. papaya cultivar by UPLC-TripleTOF-ESI-MS and to investigate the sub-acute oral toxicity in Sprague Dawley rats at doses of 0.01, 0.14 and 2 g/kg by examining the general behavior, clinical signs, hemat...

  18. Effect of irradiation on the biochemical and organoleptic changes during the ripening of papaya and mango fruits

    International Nuclear Information System (INIS)

    Papaya and mango rot caused by fungi is a major problem during storage and marketing. Gamma irradiation treatment was used to determine its effect on the quality of papayas and mangoes irradiated at 0,5 to 0,95 kGy. The level of respiration, soluble solids, texture, vitamin C and the sensorial evaluation were effectuated. The results indicate that irradiation treatment reduces significantly (p≤0,001) weakens the texture of mangoes. The content of soluble solids and vitamin C are not significantly affected by the irradiation. The sensory evaluation indicates that up to 0,95 kGy the sensorial quality is not changed. (author)

  19. AKTIVITAS ANTIBAKTERI GETAH PEPAYA KERING TERHADAP Staphylococcus aureus PADA DANGKE [Antibacterial Activity of Dried Papaya Latex toward Staphylococcus aureus in Dangke

    Directory of Open Access Journals (Sweden)

    Rifah Hestyani Arum

    2014-06-01

    Full Text Available Dangke is a traditional milk curd product, made by coagulation of milk using fresh papaya latex. This product is usually kept at room temperature (27-30C until consumption. Dried papaya latex was used in this study to produce dangke, and its effect to S. aureus was determined by direct contact in TSB and dangke. Fresh papaya latex was dried using vacuum oven at 50-55C for 22 hours. Dried papaya latex at a concentration of 2.7x10-3 g/100 mL could reduce S. aureus approximately 1 log CFU/mL in TSB after 24 hours. Dried papaya latex and papain could maintain the S. aureus number in dangke within 24 hours storage at room temperature. The antibacterial activity of non-proteolytic compound of papaya latex, i.e ethanolic extract of papaya latex was determined by macrodilution method, resulted an the MIC90 of 8 mg/mL. The cell membrane leakage after exposure was detected by measuring the optical density of bacterial supernatant at 260 nm. The result showed that exposure to increasing antibacterial concentration resulted in increasing of optical density of S. aureus supernatant, indicating that the antibacterial caused the S. aureus membrane leakage. Fluorescence microscopy imaging showed that S. aureus exposure to antibacterial caused membrane leakage thus gave Propidium Iodide (PI chance to penetrate into the cell, as indicated by changing of fluorescence color from green to red.

  20. Fractionation and purification of the enzymes stored in the latex of Carica papaya.

    Science.gov (United States)

    Azarkan, Mohamed; El Moussaoui, Anouar; van Wuytswinkel, Delphine; Dehon, Graldine; Looze, Yvan

    2003-06-25

    The latex of the tropical species Carica papaya is well known for being a rich source of the four cysteine endopeptidases papain, chymopapain, glycyl endopeptidase and caricain. Altogether, these enzymes are present in the laticifers at a concentration higher than 1 mM. The proteinases are synthesized as inactive precursors that convert into mature enzymes within 2 min after wounding the plant when the latex is abruptly expelled. Papaya latex also contains other enzymes as minor constituents. Several of these enzymes namely a class-II and a class-III chitinase, an inhibitor of serine proteinases and a glutaminyl cyclotransferase have already been purified up to apparent homogeneity and characterized. The presence of a beta-1,3-glucanase and of a cystatin is also suspected but they have not yet been isolated. Purification of these papaya enzymes calls on the use of ion-exchange supports (such as SP-Sepharose Fast Flow) and hydrophobic supports [such as Fractogel TSK Butyl 650(M), Fractogel EMD Propyl 650(S) or Thiophilic gels]. The use of covalent or affinity gels is recommended to provide preparations of cysteine endopeptidases with a high free thiol content (ideally 1 mol of essential free thiol function per mol of enzyme). The selective grafting of activated methoxypoly(ethylene glycol) chains (with M(r) of 5000) on the free thiol functions of the proteinases provides an interesting alternative to the use of covalent and affinity chromatographies especially in the case of enzymes such as chymopapain that contains, in its native state, two thiol functions. PMID:12767335

  1. Informativeness of minisatellite and microsatellite markers for genetic analysis in papaya.

    Science.gov (United States)

    Oliveira, G A F; Dantas, J L L; Oliveira, E J

    2015-10-01

    The objective of this study was to evaluate information on minisatellite and microsatellite markers in papaya (Carica papaya L.). Forty minisatellites and 91 microsatellites were used for genotyping 24 papaya accessions. Estimates of genetic diversity, genetic linkage and analyses of population structure were compared. A lower average number of alleles per locus was observed in minisatellites (3.10) compared with microsatellites (3.57), although the minisatellites showed rarer alleles (18.54 %) compared with microsatellite (13.85 %). Greater expected (He = 0.52) and observed (Ho = 0.16) heterozygosity was observed in the microsatellites compared with minisatellites (He = 0.42 and Ho = 0.11), possibly due to the high number of hermaphroditic accessions, resulting in high rates of self-fertilization. The polymorphic information content and Shannon-Wiener diversity were also higher for microsatellites (from 0.47 to 1.10, respectively) compared with minisatellite (0.38 and 0.85, respectively). The probability of paternity exclusion was high for both markers (>0.999), and the combined probability of identity was from 1.65(-13) to 4.33(-38) for mini- and micro-satellites, respectively, which indicates that both types of markers are ideal for genetic analysis. The Bayesian analysis indicated the formation of two groups (K = 2) for both markers, although the minisatellites indicated a substructure (K = 4). A greater number of accessions with a low probability of assignment to specific groups were observed for microsatellites. Collectively, the results indicated higher informativeness of microsatellites. However, the lower informative power of minisatellites may be offset by the use of larger number of loci. Furthermore, minisatellites are subject to less error in genotyping because there is greater power to detect genotyping systems when larger motifs are used. PMID:26280323

  2. Operacin y movilizacin. Formas de accin colectiva pre elticas en la Falange Nacional chilena (1935-1957

    Directory of Open Access Journals (Sweden)

    Jorge Vergara Vidal

    2012-01-01

    Full Text Available El presente texto desarrolla el concepto de operacin como una forma especfica de accin colectiva, se la considera como una movilizacin organizada de recursos acotados a objetivos y tiempos determinados. En el anlisis de la Falange Nacional chilena es posible distinguir, entre los periodos temprano y tardo de su vida como organizacin, la manera como van funcionando y produciendo efectos, por lo menos dos tipos de operaciones, caracterizadas una como doctrinaria y la otra como pragmtica. Los principales efectos de la performance de estos tipos se ubican en la concepcin e implementacin del modelo orgnico del partido y la figura vincular del agente poltico o adscriptor asociado a dicho modelo.

  3. La familia Hemiaulaceae (Bacillariophyceae de las aguas marinas chilenas The family Hemiaulaceae (Bacillariophyceae from marine Chilean waters

    Directory of Open Access Journals (Sweden)

    PATRICIO RIVERA

    2003-12-01

    Full Text Available La familia Hemiaulaceae comprende cuatro gneros, sealados previamente para las aguas chilenas con las siguientes especies: Cerataulina pelagica (Cleve Hendey, Hemiaulus sinensis Greville, H. membranaceus Cleve, Climacodium biconcavum Cleve, Eucampia antarctica (Castracane Mangin, E. cornuta (Cleve Grunow y E. zodiacus Ehrenberg. Sin embargo, el anlisis con microscopa fotnica y electrnica de muestras recolectadas en aguas marinas chilenas que contenan representantes de esta familia (incluyendo la mayora de aquellas estudiadas anteriormente por otros investigadores nacionales revel que (1 el gnero Cerataulina est representado por C. pelagica, distribuida a lo largo de la costa chilena entre Arica por el norte y el Estrecho de Magallanes por el sur, (2 el gnero Eucampia est representado por cuatro taxa: E. zodiacus f. cylindrocornis Syvertsen (sealada en trabajos anteriores como E. zodiacus f. zodiacus, E. zodiacus f. recta Rivera. Avaria & Cruces f. nov. (descrita aqu, E. cornuta y E. antarctica. Los primeros tres taxa se distribuyen en la zona central y norte de Chile, mientras que E. antarctica es propia de las aguas antrticas desde el Estrecho de Magallanes al sur, (3 las citas anteriores de Hemiaulus sinensis, H. membranaceus y Climacodium biconcavum para las aguas chilenas corresponden a determinaciones errneas de Eucampia zodiacus f. recta, f. nov. Se entregan descripciones de los taxa encontrados y fotografas obtenidas con los microscopios fotnico y electrnicos que ilustran sus principales caractersticas morfolgicasThe Family Hemiaulaceae comprises four genera, all of which have been previously reported for coastal waters off Chile, and represented by the following taxa: Cerataulina pelagica (Cleve Hendey, Hemiaulus sinensis Greville, H. membranaceus Cleve, Climacodium biconcavum Cleve, Eucampia antarctica (Castracane Mangin, E. cornuta (Cleve Grunow and E. zodiacus Ehrenberg. However, examination with light and electron microscopy of marine samples from along the Chilean coast and the Antarctic Peninsula (including those used in earlier publications revealed that (1 the genus Cerataulina is represented by C. pelagica, and is distributed along the Chilean coast between Arica in the north and the Magellan Strait in the south, (2 the genus Eucampia comprises four taxa: E. zodiacus f. cylindrocornis Syvertsen (previously reported for Chile as E. zodiacus f. zodiacus, E. zodiacus f. recta Rivera, Avaria & Cruces (described here, E. cornuta and E. antarctica. The former three taxa occur in the central and northern coast of Chile, while E. antarctica lives in Antarctic waters south of the Magellan Strait, (3 earlier records of Hemialus membranaceus, H. sinensis and Climacodium biconcavum in Chilean waters are misidentifications of Eucampia zodiacus f. recta Rivera & Avaria f. nov. A short description is included for each taxon, and photographs provide information about the main morphological characteristics

  4. Perfil Antropométrico de Jugadoras Chilenas de Fútbol Femenino Anthropometric Profile of Female Football - Soccer Chilean Players

    OpenAIRE

    Atilio Aldo Almagiá Flores; Fernando Rodríguez Rodríguez; Fernando Omar Barrraza Gómez; Pablo José Lizana Arce; Carlos Alberto Jorquera Aguilera

    2008-01-01

    Se evaluó un total de 43 jugadoras de fútbol, 26 seleccionadas chilenas sub 20 y 17 jugadoras del plantel campeón de fútbol universitario 2007 de la Pontificia Universidad Católica de Valparaíso (PUCV), todas ellas bajo su consentimiento informado. Se evaluó utilizando el protocolo de mareaje y medición de la International Society for the Avancement in Kineanthropometric (IS AK), en condiciones normales de temperatura, a primera hora de la mañana y después del vaciado urinario, por evaluadore...

  5. Estudiantes con discapacidad en una universidad chilena: desafíos de la inclusión

    OpenAIRE

    Gabriela Paulina VILLAFAÑE HORMAZÁBAL; Angélica Aurora CORRALES HUENUL; Valentina Javiera SOTO HERNÁNDEZ

    2016-01-01

    El propósito de este artículo es dar cuenta de una investigación acerca de las condiciones de inclusión para los estudiantes con discapacidad en una universidad chilena. La investigación tiene un diseño cuantitativo, descriptivo y transversal. Para la recolección de datos se elaboró una encuesta que fue aplicada a 38 estudiantes con discapacidad. Los principales resultados revelan un alto nivel de retención de los estudiantes, quienes presentan una percepción positiva de su inclusión en la vi...

  6. Interculturalidad y ciencias de la educación en lengua de señas chilena

    OpenAIRE

    Carolina-Alejandra-de-Lourdes Becerra-Sepúlveda

    2013-01-01

    Los paradigmas de intervención orales para la cultura sorda afectan la percepción sobre la lengua de señas Chilena . Esto provoca debates emanados de una subvaloración, dadas sus particularidades visuales: iconicidad e isomorfismo, subvaloración nacida de una incomprensión en la lectura de investigaciones internacionales (por escasez de estudios nacionales), especialmente acerca del lenguaje corporeizado. Esto se contradice con nuevas investigaciones en psicolingüística cognitiva acer-ca de e...

  7. La familia Hemiaulaceae (Bacillariophyceae) de las aguas marinas chilenas / The family Hemiaulaceae (Bacillariophyceae) from marine Chilean waters

    Scientific Electronic Library Online (English)

    PATRICIO, RIVERA; SERGIO, AVARIA; FABIOLA, CRUCES.

    2003-12-01

    Full Text Available La familia Hemiaulaceae comprende cuatro gneros, sealados previamente para las aguas chilenas con las siguientes especies: Cerataulina pelagica (Cleve) Hendey, Hemiaulus sinensis Greville, H. membranaceus Cleve, Climacodium biconcavum Cleve, Eucampia antarctica (Castracane) Mangin, E. cornuta (Cle [...] ve) Grunow y E. zodiacus Ehrenberg. Sin embargo, el anlisis con microscopa fotnica y electrnica de muestras recolectadas en aguas marinas chilenas que contenan representantes de esta familia (incluyendo la mayora de aquellas estudiadas anteriormente por otros investigadores nacionales) revel que (1) el gnero Cerataulina est representado por C. pelagica, distribuida a lo largo de la costa chilena entre Arica por el norte y el Estrecho de Magallanes por el sur, (2) el gnero Eucampia est representado por cuatro taxa: E. zodiacus f. cylindrocornis Syvertsen (sealada en trabajos anteriores como E. zodiacus f. zodiacus), E. zodiacus f. recta Rivera. Avaria & Cruces f. nov. (descrita aqu), E. cornuta y E. antarctica. Los primeros tres taxa se distribuyen en la zona central y norte de Chile, mientras que E. antarctica es propia de las aguas antrticas desde el Estrecho de Magallanes al sur, (3) las citas anteriores de Hemiaulus sinensis, H. membranaceus y Climacodium biconcavum para las aguas chilenas corresponden a determinaciones errneas de Eucampia zodiacus f. recta, f. nov. Se entregan descripciones de los taxa encontrados y fotografas obtenidas con los microscopios fotnico y electrnicos que ilustran sus principales caractersticas morfolgicas Abstract in english The Family Hemiaulaceae comprises four genera, all of which have been previously reported for coastal waters off Chile, and represented by the following taxa: Cerataulina pelagica (Cleve) Hendey, Hemiaulus sinensis Greville, H. membranaceus Cleve, Climacodium biconcavum Cleve, Eucampia antarctica (C [...] astracane) Mangin, E. cornuta (Cleve) Grunow and E. zodiacus Ehrenberg. However, examination with light and electron microscopy of marine samples from along the Chilean coast and the Antarctic Peninsula (including those used in earlier publications) revealed that (1) the genus Cerataulina is represented by C. pelagica, and is distributed along the Chilean coast between Arica in the north and the Magellan Strait in the south, (2) the genus Eucampia comprises four taxa: E. zodiacus f. cylindrocornis Syvertsen (previously reported for Chile as E. zodiacus f. zodiacus), E. zodiacus f. recta Rivera, Avaria & Cruces (described here), E. cornuta and E. antarctica. The former three taxa occur in the central and northern coast of Chile, while E. antarctica lives in Antarctic waters south of the Magellan Strait, (3) earlier records of Hemialus membranaceus, H. sinensis and Climacodium biconcavum in Chilean waters are misidentifications of Eucampia zodiacus f. recta Rivera & Avaria f. nov. A short description is included for each taxon, and photographs provide information about the main morphological characteristics

  8. EFECTO DEL ETILENO SOBRE EL ACC Y ACC OXIDASA EN LA MADURACION DE PAPAYA 'MARADOL'

    Directory of Open Access Journals (Sweden)

    Javier De la Cruz

    2010-01-01

    Full Text Available Frutos de papaya 'Maradol' (Carica papaya L. fueron tratados con etileno en aire sintético (0, 100, 300 y 500 uL L-1 en contenedores de fibra de vidrio a diferentes tiempos de exposición (8, 16 y 24 h. Después del tratamiento, los frutos fueron transferidos para su maduración a temperatura ambiente (25 °C. Los análisis efectuados en cada tratamiento fueron: firmeza, azúcares reducto-res, sólidos solubles, acidez titulable, color de cáscara y pulpa (Hue, velocidad de producción de etileno (VPE, contenido de ácido 1-aminociclopropano-1-carboxílico (ACC y actividad de la enzima formadora de etileno (ACC oxidasa. Los resultados indicaron que el primer cambio notable fue el color en la cáscara (amarillo y en la pulpa (naranja, típicos en un fruto listo para el consumo. Los frutos expuestos a 500 uL L-1 de etileno por 16 y 24 h mostraron un pico climatérico un día después del tratamiento, mientras que los frutos expuestos por solamente 8 h mostraron su pico climatérico 2 d después de la exposición al etileno. Las papayas expuestas a 500 uL L-1 de etileno por 16 y 24 h produjeron bajos valores de Hue en menos tiempo que el testigo, y su contenido de ACC aumentó a 2.7 nmol g-1 h-1, la actividad de la ACC oxidasa a 3 nL g-1 h-1, y la velocidad de producción de etileno (VPE a 24 nL g-1 h-1. Estas condiciones permitieron que se alcanzaran valores de firmeza de 5.2 kgf cm-2 y 12 % de sólidos solubles a los 6 d a 25 °C. El contenido de azúcares reductores, valores de pH y la acidez no mostraron diferen-cias significativas (P > 0.05 entre tratamientos. Los frutos testigo alcanzaron valores similares después de 8 d de maduración directa. Los resultados sugieren que es posible acelerar la maduración de papaya 'Maradol' de 8 a 3 d por exposición a atmósferas con etileno.

  9. INTERACCIÓN DE HONGOS MICORRÍZICOS ARBUSCULARES Y FERTILIZACIÓN FOSFATADA EN PAPAYA

    Directory of Open Access Journals (Sweden)

    Evangelina Esmeralda Qui\\u00F1ones-Aguilar

    2012-01-01

    Full Text Available Los estudios realizados sobre la simbiosis que forman los hongos micorrízicos arbusculares (HMA con diversas plantas, han revelado la importancia de estos endófitos en distintos aspectos relacionados con su nutrición y protección. Así, para la producción de frutales cultivados en vivero, el uso de HMA es una alternativa para obtener plantas más sanas y vigorosas en menor tiempo para su establecimiento en campo. En los suelos, el fósforo (P no se encuentra fácilmente disponible para las plantas, por lo que éstas han desarrollado algunas estrategias para absorberlo, como: cambios morfológicos, bioquímicos y moleculares en la raíz, además de establecer asociaciones con HMA, los cuales por medio de sus hifas promueven la absorción y transporte del P. En la presente investigación se evaluó la respuesta de papaya (Carica papaya L. a la inoculación con la cepa de HMA Glomus sp. Zac-2 y con fertilización fosfatada. Se estableció un experimento trifactorial mixto (2 × 3 × 4. Los factores estudiados fueron: inoculación micorrízica, fuente y dosis de P. Se evaluaron las variables: altura de planta (AP, diámetro de tallo (DT, área foliar (AF, volumen radical (VR, peso seco radical (PSR, peso seco de follaje (PSF y porcentaje de colonización micorrízica (PCM. Los resultados obtenidos mostraron diferencias significativas (Tukey, P £ 0.05; las plantas inoculadas mostraron incrementos en crecimiento con respecto a las plantas sin inocular de 486.51% en AP; 594.31% en DT; 1084.61% en AF; 6962.35% en VR; 13591.43% en PSR y 4992.03% en PSF. La respuesta de las plantas a la fertilización fue variable y ésta dependió de la dosis y fuente de P y de la interacción de ambos factores con los HMA. Los resultados mostraron que las plantas de papaya responden positivamente a estos microorganismos, lo que les permite aprovechar mejor el P disponible del suelo y con ello favorecer su adaptación en campo. Esto sugiere que los HMA son benéficos, en condiciones de sustrato libre de otros microorganismos, durante la etapa de producción de plántulas de papaya en vivero.

  10. Papaya ringspot virus coat protein gene for antigen presentation Escherichia coli

    Czech Academy of Sciences Publication Activity Database

    Chatchen, S.; Juříček, Miloslav; Rueda, P.; Kertbundit, Sunee

    2006-01-01

    Roč. 39, č. 1 (2006), s. 16-21. ISSN 1225-8687 Grant ostatní: Thai Research Fund(TH) BT-B-06-PG-14-4503 Institutional research plan: CEZ:AV0Z50380511 Source of funding: V - iné verejné zdroje Keywords : antigen presentation * canine parvo virus * epitope * papaya ringspot virus Subject RIV: EF - Botanics Impact factor: 1.465, year: 2006 http://www.jbmb.or.kr/view_article.php3?cont=jbmb&kid=174&mid=3&pid=3

  11. Anglicismos y aculturación en la sociedad chilena (English loanwords: acculturation in chilean society

    Directory of Open Access Journals (Sweden)

    Constanza Gerding Salas

    2012-06-01

    Full Text Available La implementación del modelo económico neoliberal estadounidense enChile hace cuatro décadas y la participación del país en la globalización han provocado cambios fundamentales en la sociedad. De hecho, los efectos de la adopción de dicho paradigma se reflejan en diversos ámbitos, incluido el del uso de la lengua. Por esta razón, resulta de interés para lingüistas y traductores indagar acerca de la influencia del idioma inglés y de la cultura estadounidense en la sociedad chilena. De allí que los objetivos de este estudio fueron constatar la presencia de préstamos del inglés en la prensa escrita, para describirlos, determinar su frecuencia de uso e inferir las causas de su adopción. Para lograr estos objetivos se clasificaron de forma manual y semiautomática préstamos del inglés extraídos de periódicos entre enero de 2003 y diciembre de 2009. Se analizó las unidades recopiladas según sus características formales, frecuencia de aparición y áreas del saber a las que pertenecían. Se determinó que los préstamos del inglés se adoptan más que adaptan y que tienen una fuerte presencia en la redacción periodística actual, específicamente en economía, computación, deportes y cultura. Además, se pudo establecer que su uso estaría determinado por cuatro factores: vacío denominativo, prestigio social, economía lingüística y preferencia de los usuarios. Finalmente, se concluyó que la lengua inglesa y la cultura estadounidense tienen una presencia significativa en la prensa chilena. (The implementation of the US-inspired neoliberal economic model in Chile four decades ago and the country’s participation in the globalization process have led to fundamental societal changes. In fact, the effect of the adoption of this paradigm is reflected in several areas, including that of the use of the language. For this reason, it is interesting for linguists and translators to find out the influence of the English language and American culture on Chilean society. Hence, the objectives of this study were to determine the presence of English loanwords in the press in order to describe them, determine their frequency of use and infer the causes of their adoption. To achieve these objectives, English borrowings collected from newspapers between January 2003 and December 2009 were classified both manually and semi-automatically. Then these Anglicisms were analyzed from the perspective of their formal characteristics, their frequency of use and the areas of knowledge to which they belonged. English loanwords were found to be adopted rather than adapted; it was also established that they have a strong presence in today’s journalistic writing, specifically in economics, computer science, sports and culture. Furthermore, their use is thought to be determined by four main factors: the lack of a counterpart in Spanish, social prestige, linguistic economy and user preference. Finally, it was concluded that the English language and American culture have a significant presence in the Chilean press.

  12. Pengaruh Penambahan Ammonium Sulfat (NH4)2SO4 dan Waktu Perendaman Buffer Fosfat terhadap Perolehan Crude Papain Dari Daun Papain (Carica Papaya, L.)

    OpenAIRE

    Alviyulita, Mitha

    2016-01-01

    Papaya leaves is a plant that rich in benefits. Papaya leaves contains papain enzyme which is a protease enzyme that very helpful for the industry. This study aims to determine the effect of the saturation level of ammonium sulfate (NH4)2SO4 and immersion time with phosphate buffer to get yield of protease from crude papaya. In this study varied immersion time with phosphate buffer is 0, 12, 24, and 36 hours and the saturation level of ammonium sulfate (NH4)2SO4 is 40, 50, 60, 70, 80, and 90 ...

  13. Revisiting the enzymes stored in the laticifers of Carica papaya in the context of their possible participation in the plant defence mechanism.

    Science.gov (United States)

    El Moussaoui, A; Nijs, M; Paul, C; Wintjens, R; Vincentelli, J; Azarkan, M; Looze, Y

    2001-04-01

    In the tropical species Carica papaya, the articulated and anastomosing laticifers form a dense network of vessels displayed in all aerial parts of the plant. Damaging the papaya tree inevitably severs its laticifers, eliciting an abrupt release of latex. Besides the well-known cysteine proteinases, papain, chymopapain, caricain and glycyl endopeptidase, papaya latex is also a rich source of other enzymes. Together, these enzymes could provide an important contribution to plant defence mechanisms by sanitising and sealing the wounded areas on the tree. PMID:11361091

  14. Descripcin multidimensional de la poblacin carcelaria chilena / A multi-dimensional description of Chilean inmates

    Scientific Electronic Library Online (English)

    Elizabeth, Len-Mayer; Mara Soledad, Corts; Jorge, Folino.

    2014-01-01

    Full Text Available A pesar que el conocimiento de las caractersticas de los penados es un insumo relevante para planificar servicios de salud en el mbito carcelario, no se contaba en Chile con informacin representativa. En este estudio de corte transversal se describe poblacin penada chilena en sus dimensiones soc [...] iodemogrficas, criminolgicas y clnicas. Se evaluaron 209 sujetos alojados en el Centro de Cumplimiento Penal de la V Regin, Chile, utilizando datos de mltiples fuentes, entrevistas video-registradas y los instrumentos Hare Psychopaty Checklist - Revised y la Gua de Evaluacin de Riesgo de Reincidencia Violenta: HCR-20. La prevalencia de trastorno de personalidad antisocial fue del 67% y la de psicopata, del 13%. Entre los factores histricos de riesgo de violencia, sobresalieron la historia de violencia previa y el abuso de sustancias. Entre los factores clnicos de riesgo de violencia, se destac la impulsividad. Abstract in english The knowledge of the characteristics of the Chilean convict population is of main importance in order to devise Health services in this area, nevertheless there was no representative information on the subject in Chile. This transversal design study describes convicted population in its socio-demogr [...] aphic, criminological, and clinical dimensions. We evaluated two hundred and nine subjects who were inmates in a jail of the 5th Region of Chile, using multiple data sources, video-recoded interviews and the instruments Hare Psychopaty Checklist - Revised and the HCR-20. Findings show that the prevalence of anti-social personality disorders was 67% and that the psychopathic disorder prevalence was 13%.Tthe existence of criminal and drug abuse records were among historical violence risk factors. Impulsiveness was among the clinical risk factors leading to violence

  15. INCORPORACIÓN DEL PAISAJE EN LA GESTIÓN DEL TERRITORIO DE LA PATAGONIA CHILENA

    Directory of Open Access Journals (Sweden)

    MARÍA DOLORES MUÑOZ

    2014-01-01

    Full Text Available Este artículo presenta una metodología para el análisis y gestión del paisaje en la Patagonia chilena, un territorio que se destaca por la belleza y diversidad de los paisajes del agua generados por la presencia de grandes lagos, ríos, fiordos, humedales y campos de hielo. El primer caso de estudio es una investigación para evaluar las potencialidades turísticas y territoriales de los paisajes del agua en la cuenca del río Baker. Se utiliza una metodología interdisciplinaria y técnicas de medición, cualitativa y cuantitativa, que permitieran analizar los valores espaciales, ambientales, territoriales y culturales asociados al paisaje. Un aspecto esencial fue incorporar la valoración social del paisaje. El segundo caso, ubicado en el estrecho de Magallanes, corresponde a una metodología de análisis territorial como base para diseñar un Plan de Manejo Turístico en el Área Marina Protegida Francisco Coloane (que incluye al Parque Marino Francisco Coloane. Comprende el análisis de las condiciones ambientales, las características del paisaje y potencialidades del turismo de intereses especiales asociado al paisaje para respaldar la propuesta de gestión. En ambos casos, la metodología considera al paisaje como principal recurso territorial y plantea una forma de gestión respaldada por principios de sustentabilidad ambiental y social.

  16. Boletín de la Academia Chilena de la Historia

    Directory of Open Access Journals (Sweden)

    JUAN GUILLERMO MUÑOZ CORREA

    2011-10-01

    Full Text Available Juan José Fernández Valdés, “El límite marítimo chileno-peruano” Luego de entregar los antecedentes desde el Tratado de Ancón, la doctrina de las 200 millas marítimas de 1947, primero por Chile y poco más de un mes después por Perú, que señaló expresamente que serían medidas “siguiendo la línea de los paralelos geográficos”, continúa analizando todas las acciones en torno al tema, no solo de estos dos países, sino los posteriores de Ecuador, Colombia, tanto decretos supremos, convenios, declaraciones, como sus ratificaciones por los respectivos estados. También repasa los puntos internacionales, como la Convención de las Naciones Unidas sobre el Derecho del Mar, signada en 1982, cuyo artículo 12 alude al método de la línea media o equidistancia para la delimitación marítima entre dos estados, exceptuando los casos en que por derechos históricos hubiera otra situación, a pesar de lo cual la posición peruana es la de adaptarse a dicho punto 12, de cuyas manifestaciones en diferentes años da cuenta este artículo, como de las consiguientes reacciones por parte de la cancillería chilena.

  17. Conocimientos y autoeficacia asociados a la prevencin del VIH y SIDA en mujeres chilenas / Knowledge and self efficacy associated to HIV and AIDS prevention in Chilean women / Conhecimentos e autoeficacia scios preveno do HIV e AIDS em mulheres chilenas

    Scientific Electronic Library Online (English)

    Natalia, Villegas Rodrguez; Lilian Marcela, Ferrer Lagunas; Rosina Cianelli, Acosta; Sarah, Miner; Loreto, Lara Campos; Nilda, Peragallo.

    2011-07-01

    Full Text Available Objetivo. Avaliar a relao existente entre conhecimentos e autoeficacia scios ao HIV/AIDS em mulheres chilenas, em mulheres chilenas em desvantagem social. Metodologia. Estudo correlacional, que utiliza a medio basal do estudo "Testando uma interveno em HIV e AIDS em mulheres chilenas", realiz [...] ada entre 2006 a 2008, que tem uma mostra de 496 mulheres entre 18 e 49 anos residentes em dois comunas de Santiago de Chile. As participantes responderam um questionrio estruturado aplicado por entrevistadoras treinadas. Este questionrio incluiu perguntas sobre dados sociodemogrficos, escala de conhecimentos de condutas de risco e autoeficacia, entre outros. Resultados. Idade mdia de 32.3+9.1 anos, 72.2% vive com seu companheiro e 42.7% possuem educao mdia completa. A pontuao mdia dos conhecimentos da infeco pelo HIV foi de 8.9+2.5, enquanto para as trs escalas empregadas para medir autoeficacia foram: "Normas dos pares" =9.8+3.6, "Inteno de reduzir condutas de risco" =12.2+3.6 e "Self Efficacy Form"=20.2+4.7. Os conhecimentos tiveram uma correlao positiva dbil com a "inteno de reduzir condutas de risco" (r=0.19; p Abstract in spanish Objetivo. Evaluar la relacin existente entre conocimientos y autoeficacia asociados al VIH/SIDA en mujeres chilenas en desventaja social. Metodologa. Estudio correlacional, que utiliza la medicin basal del estudio "Testeando una intervencin en VIH y SIDA en mujeres chilenas", realizada entre 200 [...] 6 y 2008, que tiene una muestra de 496 mujeres entre 18 y 49 aos residentes en dos comunas de Santiago de Chile. Las participantes respondieron un cuestionario estructurado aplicado por entrevistadoras entrenadas. Este cuestionario incluy preguntas sobre datos sociodemogrficos, escala de conocimientos de conductas de riesgo y autoeficacia, entre otros. Resultados. Edad promedio de 32.3+9.1 aos, 72.2% vive con su pareja y 42.7% poseen educacin media completa. La puntuacin media de los conocimientos de la infeccin por el VIH fue de 8.9+2.5, mientras que para las tres escalas empleadas para medir autoeficacia fueron: "Normas de los pares" =9.8+3.6, "Intencin de reducir conductas de riesgo" =12.2+3.6 y "Self Efficacy Form"=20.2+4.7. Los conocimientos tuvieron una correlacin positiva dbil con la "intencin de reducir conductas de riesgo" (r=0.19; p Abstract in english Objective. To assess the relationship between knowledge and self efficacy associated to HIV/AIDS in women from Chile with social disadvantages. Methodology. Correlation study that uses the baseline assessment of the "Testing an HIV and AIDS intervention in Chilean women" study, carried out between 2 [...] 006 and 2008, with a sample of 496 women between 18 and 49 years old, in 2 neighborhoods of Santiago de Chile. Participants answered a structured survey conducted by trained interviewers. The survey included questions about sociodemographic information, risk behaviors and a self-efficacy knowledge scale among others. Results. The average age was 32.3+9.1 years, 72.2% live with their partner and 42.7% have completed high school education. The mean score of HIV infection knowledge was 8.9+2.5, while the mean score for the three scales used to measure self-efficacy were: "Peer rules" =9.8+3.6, "Risk reduction intentions =12.2+3.6 and "Self Efficacy Form"=20.2+4.7. HIV knowledge had a weak positive correlation with the "Risk reduction intentions" scale (r=0.19; p

  18. VARYING GENETIC DIVERSITY OF PAPAYA RINGSPOT VIRUS ISOLATES FROM TWO TIME-SEPARATED OUTBREAKS IN JAMAICA AND VENEZUELA

    Science.gov (United States)

    The coat protein (CP) genes of eleven Jamaican Papaya ringspot virus type-p (PRSV) isolates that were collected in 1999 were cloned and sequenced. The nucleotide and amino acid sequences of these isolates were compared to each other, with a sequence of another Jamaican isolate collected after the f...

  19. Effects of chitosan-based coatings containing peppermint essential oil on the quality of post-harvest papaya fruit

    Science.gov (United States)

    Edible coatings comprised of antimicrobial polymers based on chitosan are promising technologies to preserve post-harvest fruit quality. In this study, we investigated the potential utility of a coating made from chitosan modified by N-acylation with fatty acid to preserve post-harvest papaya qualit...

  20. Managing Oriental Fruit Fly, Bactrocera dorsalis (Diptera: Tephritidae), Using Spinosad-Based Protein Bait Sprays in Papaya Orchards in Hawaii

    Science.gov (United States)

    The efficacy of GF-120 Fruit Fly Bait was evaluated as a control of female oriental fruit fly, Bactrocera dorsalis (Hendel) in papaya orchards in Hawaii. Two important components of this study were field sanitation and mass trapping using the male-specific lure methyl eugenol. Three different spray ...

  1. Evaluación de la Felicidad: Análisis Psicométrico de la Escala de Felicidad Subjetiva en Población Chilena

    Directory of Open Access Journals (Sweden)

    Pablo Vera-Villarroel

    2011-01-01

    adecuadas y altamente signifi cativas en la línea de lo esperado teóricamente. La evidencia indica adecuación de la escala para su utilización en la evaluación de este constructo en población chilena.

  2. Estrategias de marketing utilizadas por las empresas chilenas para incrementar el valor de los clientes

    Directory of Open Access Journals (Sweden)

    Pablo C. Farías Nazel

    2014-06-01

    Full Text Available El propósito de este trabajo es presentar una investigación exploratoria que busca contribuir al conocimiento acerca de la manera en que las empresas están usando estrategias demarketing para incrementar el valor de los clientes. El foco de esta investigación son las empresas chilenas. Los resultados muestran que las estrategias de marketing empleadas por las empresas chilenas presentan una significativa orientación al cliente, sin embargo, se destacan por seguir estrategias pasivas de marketing en cada uno de los tres procesos analizados para aumentar el valor de los clientes. El hecho de que una importante proporción de las empresas analizadas desarrollen estrategias pasivas, da la oportunidad para estas empresas y potenciales competidores de analizar la factibilidad de realizar estrategias más activas y científicas para abordar a sus clientes

  3. Segregacin residencial en las principales ciudades chilenas: Tendencias de las tres ltimas dcadas y posibles cursos de accin

    Directory of Open Access Journals (Sweden)

    Francisco Sabatini

    2001-12-01

    Full Text Available El patrn de segregacin residencial de las ciudades chilenas se est transformando en dos sentidos principales: est cambiando su escala geogrfica y est aumentando su malignidad. Estos cambios parecen estar afectando a la mayora de las ciudades latinoamericanas. Estn vinculados con las polticas de liberalizacin de los mercados de suelo y con los procesos de globalizacin econmica y cultural de las ltimas dcadas. En el artculo se entrega informacin emprica sobre los efectos de estos cambios en tres ciudades chilenas (Santiago, Valparaso, Concepcin; y se discute tericamente la relacin entre desigualdad social y segregacin espacial, rechazando el enfoque usual que ve a la segunda como un mero reflejo en el espacio de la primeraThe pattern of residential segregation is undergoing two crucial changes in Chilean cities: its geographical scale is shifting, and segregations malignancy is increasing. Seemingly, these changes are affecting most of Latin American cities. They are linked to the land markets liberalization policies and to the processes of economic and cultural globalization of the past decades. This paper presents empirical data showing the effects of these changes for three Chilean cities (Santiago, Valparaso, Concepcin; and theoretically discusses the relationship between social inequality and spatial segregation, rejecting the usual approach that considers the latter as a mere spatial reflection of the first

  4. Propiedades Psicométricas del Inventario de Orientación Temporal de Zimbardo en una muestra Chilena

    Directory of Open Access Journals (Sweden)

    Cristián Oyanadel

    2014-01-01

    Full Text Available La percepción del tiempo es una capacidad esencial para la adaptación del ser humano al ambiente. Un as - pecto de su estudio, la orientación temporal, caracteriza la actitud de las personas hacia el presente, pasado y futuro. e l Z tp I ha sido adaptado en múltiples países, y se han descrito aplicaciones en psicología clínica y de la salud. Este estudio busca adaptar y describir las propiedades del instrumento en una muestra chilena. Se reclutaron 604 participantes, entre 18 y 70 años. Las propiedades de los ítems y el análisis factorial explora - torio, señalan que es posible mantener la estructura original del instrumento con cinco factores. Reubicando 6 ítems el Alpha de Cronbach va desde .59 para Pasado Positivo a .80 para Pasado Negativo y Futuro. Son discutidas diferencias de género y edad. Siendo necesario otros estudios de validación, se establece como test útil para investigar la orientación temporal en población chilena.

  5. Beverages of lemon juice and exotic noni and papaya with potential for anticholinergic effects.

    Science.gov (United States)

    Gironés-Vilaplana, Amadeo; Valentão, Patrícia; Andrade, Paula B; Ferreres, Federico; Moreno, Diego A; García-Viguera, Cristina

    2015-03-01

    Lemon (Citrus limon (L.) Burm. f.) juice beverages enriched either with noni (Morinda citrifolia L.) (LN) or papaya (Carica papaya L.) (LP), were characterized by HPLC-DAD-ESI/MS(n), the antioxidant capacity was evaluated by (DPPH·), superoxide (O2(·-)), hydroxyl radicals (·OH) and hypochlorous acid (HOCl) assays, and their potential as acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitors was also assessed. The fruits are rich in a wide range of bioactive phenolics. Regarding DPPH·, ·OH and HOCl assays, the LP displayed strong activity, and LN was the most active against O2(·-). Concerning cholinesterases, LP was the most active, mainly due to lemon juice contribution. The effect on the cholinesterases was not as strong as in previous reports on purified extracts, but the bioactive-rich beverages offer the possibility of dietary coadjutants for daily consumption of health-promoting substances by adults with aging-related cognitive or physical disorders. PMID:25306312

  6. Molecular characterization and infectivity of Papaya leaf curl China virus infecting tomato in China.

    Science.gov (United States)

    Zhang, Hui; Ma, Xin-ying; Qian, Ya-juan; Zhou, Xue-ping

    2010-02-01

    Papaya leaf curl China virus (PaLCuCNV) was previously reported as a distinct begomovirus infecting papaya in southern China. Based on molecular diagnostic survey, 13 PaLCuCNV isolates were obtained from tomato plants showing leaf curl symptoms in Henan and Guangxi Provinces of China. Complete nucleotide sequences of 5 representative isolates (AJ558116, AJ558117, AJ704604, FN256260, and FN297834) were determined to be 2738-2751 nucleotides, which share 91.7%-97.9% sequence identities with PaLCuCNV isolate G2 (AJ558123). DNA-beta was not found to be associated with PaLCuCNV isolates. To investigate the infectivity of PaLCuCNV, an infectious clone of PaLCuCNV-[CN:HeNZM1] was constructed and agro-inoculated into Nicotiana benthamiana, N. tabacum Samsun, N. glutinosa, Solanum lycopersicum and Petunia hybrida plants, which induced severe leaf curling and crinkling symptoms in these plants. Southern blot analysis and polymerase chain reaction (PCR) indicated a systemic infection of test plants by the agro-infectious clone. PMID:20104645

  7. Nutritional status and fruit production of Carica papaya as a function of coated and conventional urea

    Directory of Open Access Journals (Sweden)

    Gabriel B. da Silva Júnior

    2016-04-01

    Full Text Available ABSTRACT As a strategy to minimize N losses in the soil, mineral N sources, such as polymer-coated urea, have been studied as possibility to increase the synchronization of N release by the fertilizer and its absorption by plants. Thus, this study aimed to evaluate the contents of macronutrients and the production of Formosa papaya as a function of sources and doses of N fertilizer applied as top-dressing in the region of Bom Jesus-PI, Brazil. The treatments were arranged in a 2 x 4 factorial scheme corresponding to N sources (coated urea and conventional urea and N doses (350, 440, 530 and 620 g plant-1 of N, with four replicates and four plants per plot. The contents of macronutrients in the leaf dry matter and fruit production were evaluated. The sources and doses of top-dressing N fertilization incremented the leaf contents of macronutrients and the production of Formosa papaya hybrid Caliman 01. Under the experimental conditions and based on the macronutrient contents considered as adequate for crop nutrition, associated with maximum fruit production (8.08 kg plant-1, the supply of 525 g of N plant-1 is recommended in the form of coated urea.

  8. EFFECT OF PAPAYA LEAF JUICE ON PLATELET AND WBC COUNT IN DENGUE FEVER: A CASE REPORT

    Directory of Open Access Journals (Sweden)

    Lakshmiprasad L Jadhav

    2013-08-01

    Full Text Available Dengue fever caused by dengue viruses (dengue 1–4 having Aedes aegypti mosquito as their principal vector, causes symptoms such as sudden onset of fever, headache, retro-orbital pain  and back pain along with severe myalgia due to which dengue fever is also known as “break-bone fever.” Laboratory findings include leukopenia, thrombocytopenia and in many cases, serum aminotransferase elevations. dengue hemorrhagic fever (DHF or dengue shock syndrome (DSS may occur as a complication of dengue fever.A pilot study and a randomized controlled trial showed that administration of papaya leaf juice was beneficial in dengue patients in elevating the total white cell counts and platelet counts. Based on this report, a dengue patient with thrombocytopenia and leukopenia was treated in a tertiary Ayurveda hospital. The patient was administered papaya leaf juice in the dose of 25 ml twice daily along with conventional line of management for a period of eight days. There was remarkable improvement in the subjective symptoms and the white blood cell count and platelet count were restored to normalcy.

  9. Kinetic constants for the hydrolysis of aggrecan by the papaya proteinases and their relevance for chemonucleolysis.

    Science.gov (United States)

    Dekeyser, P M; Buttle, D J; Devreese, B; Van Beeumen, J; Demeester, J; Lauwers, A

    1995-07-10

    The four known proteinases from papaya latex, namely papain (EC 3.4.22.2), chymopapain (EC 3.4.22.6), caricain (EC 3.4.22.30), and glycyl endopeptidase (EC 3.4.22.25), were purified to homogeneity and fully characterized by single radial immunodiffusion and active-site titration. A modified HPLC gel permeation assay was used to determine the kinetic constants for aggrecan hydrolysis by the papaya proteinases. The disappearance of intact aggrecan monomer was first-order, indicating that for the four enzymes studied the Km was much larger than 0.5 microM and that kcat/Km = 1.2 +/- 0.1 x 10(6) M-1 s-1 for chymopapain, 1.20 +/- 0.08 x 10(6) M-1 s-1 for caricain, 0.90 +/- 0.02 x 10(6) M-1 s-1 for papain, and 0.120 +/- 0.005 x 10(6) M-1 s-1 for glycyl endopeptidase. Chymodiactin, the chymopapain preparation used for chemonucleolysis, consists of a mixture of chymopapain (70%), caricain (20%), and glycyl endopeptidase (4%). The rate constant for the aggrecan hydrolysis by such a mixture was not significantly different from the rate constant for pure chymopapain. As a result of these observations, we predict that pure chymopapain could replace partially purified chymopapain preparations for chemonucleolysis. PMID:7625846

  10. Germination and biochemical changes in ‘Formosa’ papaya seeds treated with plant hormones

    Directory of Open Access Journals (Sweden)

    Rafael Fonsêca Zanotti

    2014-04-01

    Full Text Available This study aimed to investigate the effects of growth regulators on germination rates and biochemical compound concentrations in Carica papaya L. seeds (‘Formosa’ group. The seeds were harvested from fruits at maturation stages 3 and 5 (50 and 75% yellow fruit skin, respectively. The effects of 2-chloroethylphosphonic acid (CEPA, KNO3 and gibberellic acid (GA3on seed germination, germination index speed, soluble sugars, starch, lipids, soluble proteins and total proteins of the papaya seeds were evaluated. The seeds from stage 5 showed a higher rate of germination 30 days after sowing than did the seeds from stage 3. Treatment with CEPA decreased seed germination, apparently due to decreased starch mobilization; the opposite response was observed following KNO3 treatment. GA3, alone or in combination with KNO3, stimulated an increase in lipid mobilization. In general, with the exception of CEPA, all growth regulators tested were effective in overcoming seed dormancy, and KNO3 was the most effective. The seeds from stage 3 fruits treated with KNO3 or KNO3 + GA3 had higher rates of germination at 14 days.

  11. Prediction of genetic gain from selection indices for disease resistance in papaya hybrids

    Directory of Open Access Journals (Sweden)

    Marcelo Vivas

    2012-12-01

    Full Text Available In order to select superior hybrids for the concentration of favorable alleles for resistance to papaya black spot, powdery mildew and phoma spot, 67 hybrids were evaluated in two seasons, in 2007, in a randomized block design with two replications. Genetic gains were estimated from the selection indices of Smith & Hazel, Pesek & Baker, Williams, Mulamba & Mock, with selection intensity of 22.39%, corresponding to 15 hybrids. The index of Mulamba & Mock showed gains more suitable for the five traits assessed when it was used the criterion of economic weight tentatively assigned. Together, severity of black spot on leaves and on fruits, characteristics considered most relevant to the selection of resistant materials, expressed percentage gain of -44.15%. In addition, there were gains for other characteristics, with negative predicted selective percentage gain. The results showed that the index of Mulamba & Mock is the most efficient procedure for simultaneous selection of papaya hybrid resistant to black spot, powdery mildew and phoma spot.

  12. Effect of Prior Heat Stress on the Early Growth of Carica papaya

    Directory of Open Access Journals (Sweden)

    Gideon Olarewaju OKUNLOLA

    2013-12-01

    Full Text Available The experiment was carried out to determine the effects of heat stress on some growth parameters like shoot height, leaf area, fresh weight, dry weight as well as the accumulation of chlorophylls in Carica papaya. Seedlings of C. papaya were exposed to prior heat stress at 40 °C. A group of plants was placed in a Gallenkamp oven for four hours; another group of plants was placed in the oven for eight hours while the third group of plants was placed in a dark cupboard for the period of eight hours. Sampling was carried out at weekly intervals starting from seven days after treatment. Plants were randomly picked from each of the three treatments. Three replicates were used for each parameter. The results obtained from the study showed that there was an increment in the shoot height, leaf area, fresh weight and dry weight from the beginning to the end of the experimental period. However, the accumulation of chlorophylls did not follow a particular pattern. The analysis of variance carried out on the data obtained showed that heat stress had a significant effect on the petiole length, shoot height, leaf length, leaf width, leaf area, fresh weight and dry weight. Heat stress, however, did not produce a significant effect on the accumulation of chlorophylls a and b and total chlorophyll.

  13. Compositional and functional dynamics of dried papaya as affected by storage time and packaging material.

    Science.gov (United States)

    Udomkun, Patchimaporn; Nagle, Marcus; Argyropoulos, Dimitrios; Mahayothee, Busarakorn; Latif, Sajid; Müller, Joachim

    2016-04-01

    Papaya has been identified as a valuable source of nutrients and antioxidants, which are beneficial for human health. To preserve the nutritional properties after drying, appropriate storage specifications should be considered. This study aimed to investigate the quality and stability of air-dried papaya in terms of quality dynamics and behavior of bio-active compounds during storage for up to 9 months in two packaging materials: aluminum laminated polyethylene and polyamide/polyethylene. Samples with moisture content (MC) of 0.1328 g g(-1) and water activity (aw) of 0.5 were stored at 30 °C and relative humidity (RH) of 40-50%. The MC, aw, degree of browning (DB) and 5-hydroxymethylfurfural (HMF) content were found to notably increase as storage progressed. On the contrary, there was a significant decrease in antioxidant capacity (DPPH, FRAP and ABTS), total phenolic (TP) and ascorbic acid (AA) contents. Packaging in aluminum laminated polyethylene under ambient conditions was found to better preserve bio-active compounds and retard increases in MC, aw and DB, when compared to polyamide/polyethylene. PMID:26593545

  14. Control of papaya fruits anthracnose by essential oil of Ricinus communis

    Directory of Open Access Journals (Sweden)

    César Luis Siqueira Júnior

    2012-02-01

    Full Text Available The aim of this work was to investigate the potential of castor oil for the control of papaya diseases caused by the fungus Colletotrichum gloeosporioides and the bacterium Pseudomonas caricapapayae. The treatment with 1% castor oil did not significantly affect the fungal growth. The effectiveness of castor oil for the control of anthracnose was shown when 5% and 10% (v/v were used in the assays resulting in reduced mycelial growth. Fungal sporulation was strongly inhibited at 10% (v/v concentration of essential oil. The studies with the fresh fruits treated with 5% (v/v castor oil in aqueous emulsions resulted in effective reduction of pathogen spread in these fruits. No lesion was found in the fruits treated with oil, when compared to the control fruits. Castor oil showed no effect against the P. caricapapayae when tested in vitro. These results suggested the potential use of the castor bean essential oil and its fatty acids constituents for the control of anthracnose in papaya fruits.

  15. Conocimientos y autoeficacia asociados a la prevención del VIH y SIDA en mujeres chilenas Conhecimentos e autoeficacia sócios à prevenção do HIV e AIDS em mulheres chilenas Knowledge and self efficacy associated to HIV and AIDS prevention in Chilean women

    OpenAIRE

    Natalia Villegas Rodríguez; Lilian Marcela Ferrer Lagunas; Rosina Cianelli Acosta; Sarah Miner; Loreto Lara Campos; Nilda Peragallo

    2011-01-01

    Objetivo. Evaluar la relación existente entre conocimientos y autoeficacia asociados al VIH/SIDA en mujeres chilenas en desventaja social. Metodología. Estudio correlacional, que utiliza la medición basal del estudio "Testeando una intervención en VIH y SIDA en mujeres chilenas", realizada entre 2006 y 2008, que tiene una muestra de 496 mujeres entre 18 y 49 años residentes en dos comunas de Santiago de Chile. Las participantes respondieron un cuestionario estructurado aplicado por entrevista...

  16. Better Rooting Procedure to Enhance Survival Rate of Field Grown Malaysian Eksotika Papaya Transformed with 1-Aminocyclopropane-1-Carboxylic Acid Oxidase Gene

    OpenAIRE

    Rogayah Sekeli; Janna Ong Abdullah; Parameswari Namasivayam; Pauziah Muda; Umi Kalsom Abu Bakar

    2012-01-01

    A high survival rate for transformed papaya plants when transferred to the field is useful in the quest for improving the commercial quality traits. We report in this paper an improved rooting method for the production of transformed Malaysian Eksotika papaya with high survival rate when transferred to the field. Shoots were regenerated from embryogenic calli transformed with antisense and RNAi constructs of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) genes using the Agrobacterium tum...

  17. Insecticidal activity of seed extracts of Carica papaya (L.) against the fall armyworm Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae).

    OpenAIRE

    Fiqueroa Brito, R.; Huerta de la Peña, A.; Pérez-Moreno, I.; Marco, V.; Lopez Olguín, J. F.

    2011-01-01

    The present study shows that natural products from Carica papaya can be considered as a valid alternative to control pests in agriculture. The insecticide properties of the seed extracts of four cultivars of C. papaya (Maradol, Mammee, Yellow and Hawaiian) were added to an artificial insect diet. Bioassays were conducted with hexanic, acetonic and methanolic extracts at concentrations of 10, 100 and 1000ppm. All tests were performed with the first larval stage of Spodoptera frugiperda. The re...

  18. Diferencias entre la composición sectorial y ocupacional de las principales ciudades chilenas

    Directory of Open Access Journals (Sweden)

    Marcelo Lufín Varas

    2010-08-01

    Full Text Available La planifcación regional y urbana ha tendido a fjarse exclusivamente en la composición sectorial de las economías locales, asumiendo en forma implícita que la estructura de ocupaciones por actividad es similar a lo largo de un país. Si bien este supuesto parece ser cierto para el conjunto del tejido productivo, se encuentran diferencias signifcativas cuando se analizan ocupaciones específcas. Entre ellas destacan aquellas intensivas en conocimiento, las cuales tienden a estar sobrerrepresen-tadas en las principales ciudades. Se propone una metodología para el análisis de la relación existente entre la estructura de ocupaciones y la estructura sectorial y se aplica al estudio de las principales ciudades chilenas en 2002 a partir de la información del Censo de población, con el objetivo de estimar en qué medida estas estructuras divergen y si existen concentraciones relativas de ocupaciones, observándose que aquellas relacionadas con el conocimiento y la toma de decisiones empresariales tienen una presencia mayor que la esperada en el área metropolitana de Santiago.Regional and urban planning have tended to be exclusively focused on the industrial mix of local economies, implicitly assuming that the occupational mix of economic activities is similar throughout a country. Tis assumption seems to be right fom the perspective of the productive system as a whole, however signifcant diferences arise when analyzing certain occupations. Among them, knowledge-intensive occupations stand out and tend to be overrepresented in the main urban areas. A methodolog y is proposed to analyze the relationship between the occupational and industrial structures. Tis methodology is applied to the case of the principal Chilean cities in 2002 using data fom the population Census in order to estimate the extent to which these structures diverge and whether or not there are relative concentrations of occupations. Results show that the presence of managerial and knowledge-intensive occupations in the metropolitan area of Santiago is higher than expected.

  19. ANÁLISIS DE CONSULTAS A UN BUSCADOR DE LA WEB CHILENA

    Directory of Open Access Journals (Sweden)

    Ricardo Baeza-Yates

    2005-04-01

    Full Text Available Los buscadores de páginas Web son herramientas indispensables de orientación para sus usuarios, lo cual refleja la gran cantidad de personas que los utilizan diariamente. Las bitácoras (logs que registran el uso de un buscador son una valiosa fuente de información: nos hablan de las expectativas, preferencias y necesidades de las personas, es decir, qué esperan ellas encontrar en la Web. El resultado del análisis de logs de uso tiene un importante valor comercial, en particular para el comercio electrónico y la publicidad en Internet. El objetivo de este estudio es encontrar relaciones entre las consultas de los usuarios de la Web Chilena (.cl y las diversas áreas de clasificación de sus diferentes sitios. También se esperan encontrar relaciones entre los tipos de búsqueda realizados y los sitios visitados, para así conocer la necesidad que los lleva a formular sus consultas. La información obtenida está basada en los logs del buscador chileno TodoCL [1] entre febrero y marzo de 2004.Web page search engines are essential tools on users’ orientation, which is reflected by the big amount of people who are using them every day. The logs, reflecting the Web search engine uses, are a valuable source of information: they give us information about the users’ expectations, preferences and needs, which is everything that they expect to find on the Web. The results from the logs usage analysis have an important commercial value, in particular, for e-commerce and Internet publicity. The purpose of this study is to find relations between Chilean Web (.cl users’ queries and the different classification areas for its web sites. Also we expect to find relations between the search types made and the web sites visited, trying to get the need behind the query . The data used for this study was gotten from the Chilean search engine TodoCL, between February and March 2004.

  20. ‘Buscamos una voz que nos reciba’. Narrativa chilena reciente: lecturas cómplices

    Directory of Open Access Journals (Sweden)

    Daza D., Paulina

    2014-10-01

    Full Text Available This research explores certain suggested ways of reading recent Chilean narratives, focusing particularly on a corpus of works by four authors: Alvaro Bisama, Alejandra Costamagna, María José Viera-Gallo and Alejandro Zambra. It is proposed that from reception it is possible to specify certain ways of reading that manifest themselves in the degree of complicity achieved between the reader and the works, where this is defined by the reader’s cooperation or participation in the act of reading. The reader is drawn into the work by following the signs given in it, shifting from a critical to emotional reading by updating the stories through a close link between writer, the work and the reader. Thus the works are always read in a complicit way, but from different viewpoints and on different levels. Taking these nuances into account we aim to revise three ways of complicit reading: a through appropriation of the past, b politically and c dynamically.Esta investigación centra su estudio en algunas propuestas de lectura para la narrativa chilena reciente, especialmente un corpus compuesto por un conjunto de obras de cuatro autores: Álvaro Bisama, Alejandra Costamagna, María José Viera-Gallo y Alejandro Zambra. Se propone que desde la recepción es posible precisar algunas formas de lectura que se concretan pensando en el grado de complicidad que logra el lector con estas obras, determinada por su relación de colaboración o participación en el acto de leer. El lector se acercará más o menos a la obra siguiendo las señas propuestas en ella, así las formas de leer irán desde lo crítico hasta lo afectivo, actualizando los relatos a partir de un estrecho vínculo entre escritor, obra y lector. De esta forma la lectura siempre será cómplice, pero desde diferentes aspectos y en distintos grados. Teniendo en cuenta estos matices revisaremos tres formas de lectura cómplice: a de apropiación del pasado, b política y c dinámica.

  1. Prevalencia de vaginosis bacteriana en trabajadoras sexuales chilenas Prevalence of bacterial vaginosis in Chilean sex workers

    Directory of Open Access Journals (Sweden)

    Gloria Venegas

    2011-07-01

    Full Text Available OBJETIVO: Determinar la prevalencia de vaginosis bacteriana (VB en trabajadoras sexuales chilenas y relacionar los hallazgos con variables sociodemográficas, sexuales y clínicas. MÉTODO: Se estudió una muestra de 379 trabajadoras sexuales que asistían para control a Unidades de Atención y Control de Salud Sexual de Chile. A todas se las entrevistó para obtener antecedentes sociodemográficos y sexuales, se les realizó evaluación clínica que incluyó características del flujo vaginal, pH y prueba de aminas, y se les tomó una muestra vaginal para tinción de Gram. Para el diagnóstico de VB se empleó el criterio de Nugent. Los datos fueron analizados con EPI-INFO 3.4.1 y BioStat, utilizándose un grado de significación de P OBJECTIVE: To determine the prevalence of bacterial vaginosis (BV in Chilean sex workers and relate the findings to sociodemographic, sexual, and clinical variables. METHODS: A sample of 379 sex workers seen in Chilean Sexual Health Monitoring and Care Units for check-ups was studied. All of them were interviewed to obtain their sociodemographic and sexual history. A clinical examination was performed that included the characteristics of vaginal discharge, pH, and amine test. A vaginal sample was taken for Gram stain. The Nugent criteria were used for the diagnosis of BV. The data was analyzed with EPI-INFO 3.4.1 and BioStat, using a degree of significance of P < 0.005. RESULTS: BV prevalence was 69.1%. The syndrome was not associated with the sociodemographic variables, age or education. BV was less common in women (married or unmarried who lived with their partners than in single women (P < 0.001. There were no sexual variables associated with BV, whereas use of intrauterine devices was related (P < 0.0001. The presence of vaginal discharge, abnormal vaginal pH, and positive amine test were associated with infection (P < 0.0001. CONCLUSIONS: Like other studies conducted in different countries, this study observed that Chilean sex workers have a high prevalence of BV, with greater risk for women who use IUDs for birth control or women (married or unmarried who do not live with their partners.

  2. The effect of gamma irradiation on the ripening degree of papaya by comparing the changes of colour index, texture firmness and pH value

    International Nuclear Information System (INIS)

    This study is made to see the effect of gamma radiation on papaya (carica papaya) from eksotika cultivar through colour index comparison, firmness and pH value. Dose used on fruit is 0.0 kGy, 0.5 kGy, 1.0 kGy. 1.5 kGy and 2.0 kGy. All fruit was wrapped by using plastic after fruit being radiated. Sample was stored at temperature room for 14 days and will be analysed in day 1, 3, 7, 10 and 14. It was found the dose 1.0 kGy show slowest papaya experience ripening process compared to the other dose. Through radiation found that radiation can extend further papaya life span. After day 10, found that for dose 0.0, 0.5, 1.5 and 2.0 kGy suffer rot. For dose 0.0, 0.5, 1.5 and 2.0 kGy, discovered it is not suitable with the papaya in this study. Even more cleary, radiation is good for delayed the ripening rate but it is must be suitable with the papaya. (author)

  3. Manejo de plantas daninhas na cultura do mamoeiro Weed control in papaya

    Directory of Open Access Journals (Sweden)

    C.P. Ronchi

    2008-01-01

    Full Text Available Apesar de o controle de plantas daninhas na cultura do mamoeiro representar um dos componentes de grande importância do custo de produção, no mundo e no Brasil, a disponibilidade de informações sobre esse tema é escassa, o que reflete a pouca importância que tem sido dada ao assunto nas últimas décadas. Neste texto são discutidos alguns avanços no manejo integrado de plantas daninhas que têm sido alcançados em vários países, particularmente no tocante à manutenção da vegetação natural ou à introdução de leguminosas na entrelinha de cultivo. Não obstante, várias espécies de plantas daninhas foram identificadas como hospedeiras de vetores de importantes viroses à cultura, com destaque para Commelina benghalensis e Solanum americanum. Isso se reveste de grande importância para o manejo (seletivo das plantas daninhas, sobretudo na produção integrada de mamão. Também são discutidos, em especial para a região norte do Estado do Espírito Santo, a seletividade de herbicidas, os períodos de controle e as principais características e aspectos práticos sobre o manejo de plantas daninhas na cultura do mamoeiro.Despite the fact that weed control entails high costs to crop production, the availability of national and international papers regarding papaya weed control is scarce, showing that little importance has been given to this subject in the last decades. Some integrated weed management advances attamed in several countries are discussed, particularly in the use of both natural groundcover or leguminous plants in the crop interrows. However, several weed species have been identified as host plants of major virus vectors to the crop, such as C. benghalensis and S. americanum. Such information is of major importance for selective weed control, particularly in the integrated system of papaya production. The present review also examined herbicide selectivity, weedy periods as well as the main characteristics and practical aspects of weed control in papaya, focusing on the production areas of the northern Espírito Santo State.

  4. Effect of irradiation on color of minimally processed melon and papaya

    International Nuclear Information System (INIS)

    The access to nutritious food is an essential dimension of food meal. High potential for fresh-cut industry exists and ready-to-eat fruit market has grown rapidly in recent years due to the health benefits associated. Although there is many concerns to food safety other parameters like texture, taste, color and sensory acceptance are fundamental principles of acceptance to any food. Actually the use of instrumental measurements has proven to be a major predictor of sensory responses. According to many authors, the addition of different techniques should always be considered to provide additional information of the sensory aspects. Therefore the objective of this study was to evaluate the influence of irradiation on color of minimally processed melon and papaya. The fruits were purchased in a market of Sao Paulo, at the same point of ripeness and sent to the IPEN/CNEN-SP. The fruits were sanitized and manually cut into cubes of approximately 2 x 2 cm with the aid of stainless steel knives and packed in polyethylene bags. Melons and papaya were irradiated in a Multipurpose Gamma Source (IPEN - Sao Paulo - Brazil) and were divided in six groups for color analysis: Control; 0.5 kGy; 1.0 kGy, 1.5kGy, 2.0 kGy and 3.0kGy. After the treatment, the MP fruits were kept in a refrigerator at 4 deg C ± 1 deg C until the end of the analysis. The color of the samples was determined using a Minolta colorimeter CR-400 Chromameter. The parameters L*, a* and b* were evaluated. The results were statistically analyzed by analysis of variance One-Dimensional Analysis of Variance (One-Way-ANOVA) followed by Tukey test. All statistical analysis was performed using the program Graph Pad Prism 5 and adopting a significance level of 5 % (p* parameter of any dose despite the tendency to darkening observed for the group of 3.0 kGy. This fact also occurred for the chromatographic coordinates a* and b* which remained in the same tonality for all treatments (p<0.05). Current results indicate that gamma radiation can be used for keeping the color. Doses up to 3kGy were efficient for that. Future studies should consider pretreatments to minimize the darkening effects on fruits. Therefore, the results from this study suggest the use of gamma radiation as a quality alternative for keep the color of minimally processed melon and papaya. (author)

  5. Involvement of AOX and UCP pathways in the post-harvest ripening of papaya fruits.

    Science.gov (United States)

    Oliveira, M G; Mazorra, L M; Souza, A F; Silva, G M C; Correa, S F; Santos, W C; Saraiva, K D C; Teixeira, A J; Melo, D F; Silva, M G; Silva, M A P; Arrabaça, J D C; Costa, J H; Oliveira, J G

    2015-09-15

    Enhanced respiration during ripening in climacteric fruits is sometimes associated with an uncoupling between the ATP synthesis and the mitochondrial electron transport chain. While the participation of two energy-dissipating systems, one of which is mediated by the alternative oxidase (AOX) and the other mediated by the uncoupling protein (UCP), has been linked to fruit ripening, the relation between the activation of both mitochondrial uncoupling systems with the transient increase of ethylene synthesis (ethylene peak) remains unclear. To elucidate this question, ethylene emission and the two uncoupling (AOX and UCP) pathways were monitored in harvested papaya fruit during the ripening, from green to fully yellow skin. The results confirmed the typical climacteric behavior for papaya fruit: an initial increase in endogenous ethylene emission which reaches a maximum (peak) in the intermediate ripening stage, before finally declining to a basal level in ripe fruit. Respiration of intact fruit also increased and achieved higher levels at the end of ripening. On the other hand, in purified mitochondria extracted from fruit pulp the total respiration and respiratory control decrease while an increase in the participation of AOX and UCP pathways was markedly evident during papaya ripening. There was an increase in the AOX capacity during the transition from green fruit to the intermediate stage that accompanied the transient ethylene peak, while the O2 consumption triggered by UCP activation increased by 80% from the beginning to end stage of fruit ripening. Expression analyses of AOX (AOX1 and 2) and UCP (UCP1-5) genes revealed that the increases in the AOX and UCP capacities were linked to a higher expression of AOX1 and UCP (mainly UCP1) genes, respectively. In silico promoter analyses of both genes showed the presence of ethylene-responsive cis-elements in UCP1 and UCP2 genes. Overall, the data suggest a differential activation of AOX and UCP pathways in regulation related to the ethylene peak and induction of specific genes such as AOX1 and UCP1. PMID:26513459

  6. Cytogenetics of Chilean angiosperms: Advances and prospects Citogenética de angiospermas chilenas: Avances y proyecciones

    Directory of Open Access Journals (Sweden)

    PEDRO JARA-SEGUEL

    2012-03-01

    Full Text Available Cytogenetic data on Chilean angiosperms have been reported since at least eight decades ago; however, much of this information is disperse in diverse sources and is not readily available as a comprehensive document that allows having a general vision on advances and gaps in this matter. The goal of this paper is to summarize the advances and prospets on cytogenetic studies of the Chilean angiosperms based on compiled publications from 1929 to 2010. We found 78 publications supplied by four groups of Chilean researchers and some foreign specialists. Cytogenetic data have been reported for 139 Chilean angiosperm species (2.8 % of the total, which belong to 58 genera and 34 families. During 2001-2010 there was an increase in the number of publications, being available 40 reports including 95 additional species. Based on these data, we hope that such a trend can be maintained in the next decade if the current research groups and young specialists continue to be interested in the study of native plants.Los datos citogenéticos sobre angiospermas chilenas han sido reportados desde al menos ocho décadas atrás; sin embargo, mucha de esta información está dispersa en diversas fuentes y no está disponible como un documento completo que permita tener una visión general sobre los avances y vacíos en esta materia. El objetivo de este trabajo es resumir los avances y proyecciones sobre los estudios citogenéticos disponibles para angiospermas chilenas, basado en publicaciones recopiladas desde 1929 hasta el 2010. Nosotros encontramos 78 publicaciones aportadas por cuatro grupos de investigadores chilenos y por algunos especialistas extranjeros. Datos citogenéticos han sido reportados para 139 especies de angiospermas chilenas (2.8 % del total, las cuales pertenecen a 58 géneros y 34 familias. Durante los años 2001-2010, existió un incremento en el número de publicaciones estando disponibles 40 reportes que incluyen 95 especies adicionales. Basados en estos datos, esperamos que esta tendencia pueda ser mantenida en la siguiente década si los actuales grupos de investigación y especialistas jóvenes siguen interesados en estudiar plantas nativas.

  7. RNA Interference of 1-Aminocyclopropane-1-carboxylic Acid Oxidase (ACO1 and ACO2 Genes Expression Prolongs the Shelf Life of Eksotika (Carica papaya L. Papaya Fruit

    Directory of Open Access Journals (Sweden)

    Rogayah Sekeli

    2014-06-01

    Full Text Available The purpose of this study was to evaluate the effectiveness of using RNA interference in down regulating the expression of 1-aminocyclopropane-1-carboxylic acid oxidase gene in Eksotika papaya. One-month old embryogenic calli were separately transformed with Agrobacterium strain LBA 4404 harbouring the three different RNAi pOpOff2 constructs bearing the 1-aminocyclopropane-1-carboxylic acid oxidase gene. A total of 176 putative transformed lines were produced from 15,000 calli transformed, selected, then regenerated on medium supplemented with kanamycin. Integration and expression of the targeted gene in putatively transformed lines were verified by PCR and real-time RT-PCR. Confined field evaluation of a total of 31 putative transgenic lines planted showed a knockdown expression of the targeted ACO1 and ACO2 genes in 13 lines, which required more than 8 days to achieve the full yellow colour (Index 6. Fruits harvested from lines pRNAiACO2 L2-9 and pRNAiACO1 L2 exhibited about 20 and 14 days extended post-harvest shelf life to reach Index 6, respectively. The total soluble solids contents of the fruits ranged from 11 to 14° Brix, a range similar to fruits from non-transformed, wild type seed-derived plants.

  8. Efecto del calcio en aplicaciones precosecha y poscosecha sobre la severidad de antracnosis (Colletotrichum gloeosporioides y la calidad de frutos de papaya (Carica papaya

    Directory of Open Access Journals (Sweden)

    Daniel Sabor\\u00EDo

    2000-01-01

    Full Text Available Se evalu6 el efecto de aplicaciones pre- cosecha y poscosecha de calcio en papaya va- riedad "criolla" sabre la severidad de antracno- sis (CoUetotrichum gloeosporioides y varia- hIes de calidad del fruto. Los tratamientos pre- cosecha fueron 4: aspersi6n de CaCl2 al 1% Y 4% (2 aplicaciones: <40 dfas posantesis (dpa y entre 100-140 dpa con el penetrante alquilaril- polimero (NP- 7 Bayer (0.4 mIlL, CaCO3 al suelo (1 ton/ha, 70 dpa y testigo (0% Ca. El diseno experimental fue un BCA (4 repeticio- nes de 20 frutos. Los tratamientos poscosecha fueron 3: inmersiones par 5 min con 0%, 1 % Y 4% de CaCI2, con el mismo penetrante. El dise- no experimental fue un BCA (3 repeticiones de 15 frutas. Se evalu6 severidad, % calcio en cascara, brix, pH, % acidez, firmeza (cascara y pulpa y % de madurez. En la aplicaci6n de cal- cia precosecha la severidad fue: 1 % CaC12 con 6%, testigo 7%, CaCO3 9% y 4% CaC12 con 11 %, no se encontr6 que el Ca tuviera un efec-

  9. PENGGUNAAN GETAH PEPAYA DALAM SINTESIS ESTER XILITOL ASAM LEMAK (EXAL [The Use of Papaya Exudates for Fatty Acid Xilitol Synthesis

    Directory of Open Access Journals (Sweden)

    Suhardi

    2003-12-01

    Full Text Available Fatty acid xylitol-ester (FAXILE are xylitol esters of fatty acids with one to five DE (degree of esterification value. FAXILE with DE value of 3 or higher can be used as low calorie fat replacer since they have low digestive property; while the xylitol ester with DE of less than 3 can be used as emulsifier. The FAXILE synthesis experiments were carried out by esterification of xylitol with palm oil¡¦s fatty acid (POFA using papaya exudates as a lipase source. The objective of this experiment was to evaluate the potential of papaya exuadates for the synthesis of FAXILE. In this first experiment, test were carried out to determine hydrolytic activity of lipases obtained from different part of papaya plant. The test, were performed with the presence of CaO and under different pH value. The papaya exudates were used for FAXILE synthesis under an optimum condition obtained from the first experiment. Samples were drawn during incubation at 40„aC for 1,2,3, and 4 days and hydroxyl number was analyzed to confirm the ester formation. The results showed that the most active exudate was from papaya leaves, followed by exudates from fruit with hydrolytic activity of 653 and 296 ƒÝmol/g. minutes, respectively. The hydrolytic activity of the fruit exudate was optimum at pH 6.0, at 45-50„aC, with the addition of CaO 4% dry exudates. The FAXILE synthesis with acid of papaya exudates was optimum at pH 6.0, at 45„aC with molar ratio of xylitol: fatty acids was 1 to 6,3 days incubation. At this condition the conversion rate xylitol to FAXILE was 79%.

  10. Effects of papaya leaves on thrombocyte counts in dengue--a case report.

    Science.gov (United States)

    Siddique, Osama; Sundus, Ayesha; Ibrahim, Mohammad Faisal

    2014-03-01

    Dengue fever is on the rise in developing nations like India, Pakistan, Sri Lanka and Bangladesh. There is no antiviral chemotherapy or vaccine for dengue virus and management of the disease is done on supportive measures. The decline in the thrombocyte count leads to dengue haemorrhagic fever accounting for complications and mortality. Oral administration of Carica papaya leaves extract is said to have a positive impact on thrombocyte count. A 23-year-old man was administered a calculated dose for five days. Blood samples were tested for complete blood count before and after the administration of the juice. Thrombocyte count had increased from 28000/micro liter to 138000/micro liter at the end of five days. We present our experience here. PMID:24864622

  11. EVALUACIÓN DE DOS HÍBRIDOS DE PAPAYA INTRODUCIDOS EN CUBA

    Directory of Open Access Journals (Sweden)

    Maruchi Alonso

    2009-01-01

    Full Text Available En el presente trabajo se realizó la caracterización morfológica de 2 híbridos de papaya "Tainung-01" y "Scarlett Princess" introducidos en el banco de germoplasma del Instituto de Investigaciones en Fruticultura Tropical, La Habana, Cuba. Y fueron comparados con el cultivar cubano Maradol. Los resultados corroboraron que los híbridos presentan características fenotípicas del Grupo Formosa, frutos de forma elongata en las plantas hermafroditas. El peso medio de los frutos es de 1,6 kg (''Tainung-01'' y 1,7 kg (''Scarlett Princess'', de pulpa color naranja-rojiza y roja, respectivamente, así como, con una productividad entre 98,5 y 63,8 kg.planta-1, características que evidencian la posibilidad de utilizarlos en programas de mejoramiento genético del cultivo y directamente por los productores.

  12. Process variables influence on microwave assisted extraction of pectin from waste Carcia papaya L. peel.

    Science.gov (United States)

    Maran, J Prakash; Prakash, K Arun

    2015-02-01

    The objectives of the present study were to evaluate and optimize the influence of process variables such as microwave power, pH, time and solid-liquid ratio on the extraction of pectin from waste Carcia papaya L peel. The experiments were carried out based on a four factors three level Box-Behnken response surface design. A quadratic model was developed from the experimental data in order to predict the pectin yield. The optimal condition was found to be: microwave power of 512 w, pH of 1.8, time of 140 s and solid-liquid ratio of 1:15 g/ml with maximum pectin yield (25.41%). PMID:25445679

  13. Radiation preservation of foods of plant origin. III. Tropical fruits: bananas, mangoes, and papayas

    International Nuclear Information System (INIS)

    The current status of research on the use of ionizing radiation for shelf life improvement and disinfestation of fresh tropical fruits like bananas, mangoes, and papayas are reviewed. The aspects covered are influence of maturity and physiological state of the fruits on delayed ripening and tolerance to radiation; varietal responses; changes in chemical constituents, volatiles, respiration, and ethylene evolution; biochemical mechanisms of delayed ripening and browning of irradiated fruits; and organoleptic quality. The efficacy of the combination of hot water dip and radiation treatments for control of postharvest fungal diseases are considered. The immediate potential of radiation as a quarantine treatment, in place of the currently used chemical fumigants, for disinfestation of fruit flies and mango seed weevil are discussed. Future prospects for irradiation of tropical fruits are discussed in the light of experience gained from studies conducted in different countries.146 references

  14. Effect of irradiation on color of minimally processed melon and papaya

    Energy Technology Data Exchange (ETDEWEB)

    Fabbri, Adriana D.T.; Sagretti, Juliana M.A.; Hirashima, Fabiana K.; Rogovschi, Vladimir D.; Nunes, Thaise C.F.; Sabato, Susy F., E-mail: adriana.fabbri@yahoo.com.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil). Lab. de Irradiacao de Alimentos

    2013-07-01

    The access to nutritious food is an essential dimension of food meal. High potential for fresh-cut industry exists and ready-to-eat fruit market has grown rapidly in recent years due to the health benefits associated. Although there is many concerns to food safety other parameters like texture, taste, color and sensory acceptance are fundamental principles of acceptance to any food. Actually the use of instrumental measurements has proven to be a major predictor of sensory responses. According to many authors, the addition of different techniques should always be considered to provide additional information of the sensory aspects. Therefore the objective of this study was to evaluate the influence of irradiation on color of minimally processed melon and papaya. The fruits were purchased in a market of Sao Paulo, at the same point of ripeness and sent to the IPEN/CNEN-SP. The fruits were sanitized and manually cut into cubes of approximately 2 x 2 cm with the aid of stainless steel knives and packed in polyethylene bags. Melons and papaya were irradiated in a Multipurpose Gamma Source (IPEN - Sao Paulo - Brazil) and were divided in six groups for color analysis: Control; 0.5 kGy; 1.0 kGy, 1.5kGy, 2.0 kGy and 3.0kGy. After the treatment, the MP fruits were kept in a refrigerator at 4 deg C ± 1 deg C until the end of the analysis. The color of the samples was determined using a Minolta colorimeter CR-400 Chromameter. The parameters L{sup *}, a{sup *} and b{sup *} were evaluated. The results were statistically analyzed by analysis of variance One-Dimensional Analysis of Variance (One-Way-ANOVA) followed by Tukey test. All statistical analysis was performed using the program Graph Pad Prism 5 and adopting a significance level of 5 % (p<0.05), expressed as the mean results ± standard deviation. Samples of papaya and melons showed no statistical difference for the L{sup *} parameter of any dose despite the tendency to darkening observed for the group of 3.0 kGy. This fact also occurred for the chromatographic coordinates a{sup *} and b{sup *} which remained in the same tonality for all treatments (p<0.05). Current results indicate that gamma radiation can be used for keeping the color. Doses up to 3kGy were efficient for that. Future studies should consider pretreatments to minimize the darkening effects on fruits. Therefore, the results from this study suggest the use of gamma radiation as a quality alternative for keep the color of minimally processed melon and papaya. (author)

  15. Evaluación de la uniformidad del riego y efecto del fertirriego nitrogenado en un huerto de lechosa (Carica papaya L. Evaluation of irrigation uniformity and effect of nitrogen fertirrigation in a papaya orchard (Carica papaya L.

    Directory of Open Access Journals (Sweden)

    Carmen Basso

    2008-08-01

    Full Text Available En un huerto de lechosa cv. Cartagena Roja se estudió el desempeño del fertirriego nitrogenado y riego sobre el rendimiento del cultivo. Se evaluaron cuatro tratamientos de fertilización nitrogenada y tres de riego, utilizando cintas de goteo, y se registró la humedad del suelo usando un TDR y mediante gravimetría. La cosecha se inició al final del período lluvioso y se prolongó durante veinte y un semanas del período seco. Se encontró que la descarga de los emisores resultó altamente uniforme en sus distintas modalidades de operación. Los tratamientos de fertilización nitrogenada no se vieron reflejados en el rendimiento de las plantas pero sí en el adelanto de la cosecha en los tratamientos con mayor dosis de fertilizante. Los tratamientos de riego tampoco afectaron los rendimientos, lo cual se atribuyó al efecto de la humedad almacenada en el perfil durante el período húmedo.Nitrogenous fertilization and watering performance on yield of a papaya orchard cv. Red Cartagena was studied. Four treatments of nitrogenous fertilization and three of watering were evaluated using drip lines. The soil moisture was registered by TDR and gravimetry. The harvest began at the end of the rainy period and it prolonged for twenty one weeks in the dry period. It was found that the emitter discharge was highly uniform in its different operation modalities. The nitrogenous fertilization did not affect the plant yield but permitted earlier maturity of fruits. Similarly, the watering treatments did not affect the yield which was attributed to the effect of the water stored in the soil profile during the rainy period.

  16. Nitrogen fertilization sources and insecticidal activity of aqueous seeds extract of Carica papaya against Spodoptera frugiperda in maize / Fuentes nitrogenadas de fertilizacin y actividad insecticida del extracto acuoso de semilla de Carica papaya contra Spodoptera frugiperda en maz

    Scientific Electronic Library Online (English)

    Rodolfo, Figueroa-Brito; Patricia, Villa-Ayala; Jess F, Lpez-Olgun; Arturo, Huerta-de la Pea; Juan R, Pacheco-Aguilar; Miguel A, Ramos-Lpez.

    2013-12-01

    Full Text Available R. Figueroa-Brito, P. Villa-Ayala, J.F. Lpez-Olgun, A. Huerta-de la Pea, J.R. Pacheco-Aguilar y M.A. Ramos-Lpez. 2013. Fuentes nitrogenadas de fertilizacin y actividad insecticida del extracto acuoso de semilla de Carica papaya contra Spodoptera frugiperda en maz. Cien. Inv. Agr. 40(3): 567-57 [...] 7. Se estim el dao causado por el gusano cogollero del maz Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) durante la etapa vegetativa de maz cultivado con fertilizantes nitrogenados qumicos, vermicomposta, y extracto acuoso de semillas de Carica papaya L. (Caricaceae). Cada brote se infest con una larva de primer instar de S. frugiperda. Las variables evaluadas incluyen el porcentaje de germinacin. Las variables respuesta fueron el porcentaje de emergencia, longitud de la segunda y quinta hoja, dimetro de tallo y altura de la planta; as como la estimacin de dao causado por larvas de S. frugiperda al maz. Los resultados indicaron que la vermicomposta ayud a la emergencia de la semilla, en una relacin 3:1 de suelo con vermicomposta alcanz 100% de emergencia, mientras que solo con suelo, la emergencia fue de 80%; el sulfato de amonio increment el tamao de la segunda hoja y quinta hoja 89,6 y 160,4% respectivamente, aument el dimetro de tallo y la altura de la planta 290,2 y 13,3%, respecto al tratamiento donde solo se aplic agua. Las fuentes de nitrgeno estimularon que S. frugiperda ocasionara ms dao a la planta, el tratamiento con urea present 70% de dao, con fosfonitrato 62,3%, con sulfato de amonio fue de 51,8% sin extracto acuoso de semillas de C. papaya. El tratamiento con sulfato de amonio + extracto acuoso de semillas de C. papaya registr el menor dao del insecto al maz con 29,6%. Abstract in english R. Figueroa-Brito, P. Villa-Ayala, J.F. Lpez-Olgun, A. Huerta-de la Pena, J.R. Pacheco-Aguilar, and M.A. Ramos-Lpez. 2013. Nitrogen fertilization sources and insecticidal activity of aqueous seeds extract of Carica papaya against Spodoptera frugiperda in maize. Cien. Inv. Agr. 40(3): 567-577. The [...] damage caused by the fall armyworm Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) to vegetative-stage maize cultivated with chemical nitrogen fertilizers, vermicompost, and Carica papaya L. (Caricaceae) seed extract was estimated. Each shoot was infested with one first instar larva of S. frugiperda. The variables measured included the percentage of germination, length of the second and fifth leaves, stem diameter and plant height, and the estimation of damage caused by S. frugiperda larvae on maize. The results indicated that vermicompost helped seed germination on a relation 3:1 from black soil and vermicompost, the emergence was 100%, while the emergence with black soil was 80%. The ammonium sulfate increased the length of the second and fifth leaves 89.6% and 160.4% respectively, augmented the stem diameter and the plant height 290.2% and 13.3% respectively, respect to water treatment. The exogenous nitrogen sources stimulate S. frugiperda to cause more damage to the plant, the treatment urea showed 70% of damage, with phosphonitrate 62.3% and with ammonium sulfate 51.8%, when were evaluated without aqueous seed extract of C. papaya. Ammonium sulfate + aqueous extract of C. papaya seeds showed the lowest insect damage to maize with 29.6%.

  17. La experiencia chilena de retencin de estudiantes en la universidad / The Chilean Experience on University Students's Retention

    Scientific Electronic Library Online (English)

    Sebastin, Donoso D.; Gonzalo, Donoso T.; Claudio, Frites C..

    2013-06-01

    Full Text Available Se enfoca la temtica de la retencin de estudiantes en la educacin superior chilena, esencialmente referida al nivel universitario, describiendo la arquitectura del sistema y los procesos de seleccin implementados, para, en funcin de estos antecedentes, dimensionar el problema y revisar las medi [...] das ms recurrentes de mitigacin de sus efectos negativos. Se concluye con orientaciones de polticas para el sistema. Abstract in english This article focuses on the issue of student retention in higher education in Chile, particularly at the university level. It describes the system architec-ture and the selection processess implemented. Based on this background information, it measures the problem and reviews the extent of recurrent [...] negative mitigation. The article concludes with policy guidelines for the system.

  18. EXTRABLES DE CORTEZAS CHILENAS: EFECTO SOBRE LA LUMINISCENCIA DE BACTERIAS / EFFECT OF CHILEAN BARK EXTRACTS ON BACTERIAL LUMINESCENCE

    Scientific Electronic Library Online (English)

    Hernn, Poblete; Edmone, Roffael; Helmut, Miertzsch.

    Full Text Available Se determin la disminucin de la bioluminiscencia de bacterias como indicador de la actividad biolgica de extractos de corteza. Se analizaron cuatro cortezas nativas chilenas. La reduccin de la bioluminiscencia fue diferente dependiendo de la especie de corteza. El aumento del tiempo de un tratam [...] iento trmico de la corteza (24, 48, 72 y 144 horas con 103C) produjo una reduccin de la actividad biolgica de los extractos. El estudio indica que los terpenos podran ser responsables del efecto sealado Abstract in english Bioluminescence decrease of bacteria as a biological activity indicator for bark water extracts was determinate. Four Chilean barks were tested. A different reduction on bioluminescence depending on bark species was observed. Increasing time of thermal treatment of bark (24, 48, 72 and 144 hours wit [...] h 103C) produced a reduction of the biological activity of extracts. The study indicates that the terpenes could be responsible for the described effect

  19. Origen, consolidación y vigencia de la Nueva Dogmática Chilena (ca. 1955≈1970

    Directory of Open Access Journals (Sweden)

    MATUS, Jean Pierre

    2011-01-01

    Full Text Available El artículo describe la evolución de tres generaciones de profesores de Derecho penal chilenos, desde 1925 hasta 1970. Se indican las fuentes e influencias que reciben cada una de ellas y cómo influyen en las sucesivas generaciones, respectivamente. Se establecen las condiciones que incidieron en la eclosión de una gran cantidad de nuevos especialistas en Derecho penal en la generación de 1955-1970, denominada Nueva Dogmática Chilena, y su participación en la disputa entre el “sistema neoclásico” y la "teoría de la acción final". Se concluye con el análisis de la vigencia de sus aportes y de los efectos del Golpe de Estado de 1973 en su desarrollo posterior.

  20. Interculturalidad y ciencias de la educación en lengua de señas chilena

    Directory of Open Access Journals (Sweden)

    Carolina-Alejandra-de-Lourdes Becerra-Sepúlveda

    2013-08-01

    Full Text Available Los paradigmas de intervención orales para la cultura sorda afectan la percepción sobre la lengua de señas Chilena . Esto provoca debates emanados de una subvaloración, dadas sus particularidades visuales: iconicidad e isomorfismo, subvaloración nacida de una incomprensión en la lectura de investigaciones internacionales (por escasez de estudios nacionales, especialmente acerca del lenguaje corporeizado. Esto se contradice con nuevas investigaciones en psicolingüística cognitiva acer-ca de embodied cognition . El abordaje de la LSCh a partir de nuevas teorías atraviesa la interculturalidad y educación a fin de proponerse la metáfora de “la cultura en el cuerpo” como modalidad de estudio contemporáneo.

  1. GROWTH OF MACROBRACHIUM ROSENBERGII FED WITH MANGO SEED KERNEL, BANANA PEEL AND PAPAYA PEEL INCORPORATED FEEDS

    Directory of Open Access Journals (Sweden)

    P. Aarumugam

    2013-06-01

    Full Text Available The growth promoting potential of fruits wastes, mango seed kernel, banana peel and papaya peel on the freshwater prawn, Macrobrachium rosenbergii post larvae (PL was evaluated. Basal diet equated to 35% protein was prepared by using soybean meal, groundnut oilcake, horse gram and wheat flour. Each fruit waste powder was separately incorporated with basal diet at a proportion of 10%. Sunflower oil was used as lipid source. Egg albumin and tapioca flour were used as binding agents. Vitamin B-complex with Vitamin-C was also mixed. Feed without any fruit waste was served as control. M. rosenbergii PL (length: 1.2-1.4 cm; weight: 0.09- 0.13 g was fed with these feeds for a period of 90 days. Significant improvements in the nutritional indices (survival rate, weight gain, biomass index, specific growth rate and condition factor, concentrations of biochemical constituents (total protein, carbohydrate and lipid, levels of non-enzymatic antioxidants (vitamin-C and E, content of minerals (Na+ and K+, activities of digestive enzymes (protease, amylase and lipase, and profiles of essential amino acids and fatty acids were recorded in fruits wastes incorporated feeds fed PL when compared with control (P< 0.003 0.878. The overall results indicated the fact that mango seed kernel incorporated feed was produced the best performance, followed by better performance of banana peel and good performance of papaya peel. These fruits wastes incorporated feeds enhance digestive enzymes activities and act as appetizer, which in turn enhances food utilization and ultimately yielded better survival and growth of M. rosenbergii PL. Therefore, these fruits wastes have considerable potentials in sustainable development of Macrobrachium culture.

  2. Improved Immunoassay Sensitivity in Serum as a Result of Polymer-Entrapped Quantum Dots: 'Papaya Particles'.

    Science.gov (United States)

    Ranzoni, Andrea; den Hamer, Anniek; Karoli, Tomislav; Buechler, Joseph; Cooper, Matthew A

    2015-06-16

    Fluorescent labels are widely employed in biomarker quantification and diagnostics, however they possess narrow Stokes shifts and can photobleach, limiting multiplexed detection applications and compromising sensitivity. In contrast, quantum dots do not photobleach and have much wider Stokes shifts, but a paucity of robust surface attachment chemistries for bioconjugation has limited their uptake in biomedical diagnostics. We report a novel class of biofunctional fluorescent labels based on trapping of ∼10(4) quantum dots within a core nanoparticle. The doped particles act as scaffolds for generation of a multilayered shell consisting of a functionalized hydrophilic polymer with covalently attached receptors for analyte capture. These constructs, which conceptually resemble a papaya fruit, are chemically stable, remain monodispersed for >6 months in buffer, and show utility in immunoassay applications. Using monoclonal antibody fragments against nonstructural protein dengue NS1, an early biomarker for dengue fever, antibody immobilization capacity was 75-fold higher compared with traditional carbodiimide protein coupling. In the model dengue immunoassay, we observed a 15-fold lower limit of detection and 4-fold higher fluorescence intensity with the "papaya particles" compared to current "best-in-class" commercial reagents. Direct deployment in human serum allowed sensitive detection of different NS1 serotypes with lower limits of detection within the clinically relevant range (1-10 ng/mL), and sufficient specificity for identification of the dengue serotype was achieved for concentrations >10 ng/mL (DV1-3) and >50 ng/mL (DV4). The combination of chemical and physical stability and high binding capacity combined with the intrinsic advantages of quantum dots may enable more simple, robust diagnostic assays in the future. PMID:25971296

  3. Protective Effect of Carica papaya Linn Against gamma-Radiation-Induced Tissue Damage in Rats

    International Nuclear Information System (INIS)

    The present study was designed to determine the possible protective effects of the Carica papaya fruit aqueous extract (CP) against ?-radiation induced oxidative stress, biochemical and hematological alterations in male albino rats. Papaya (250 mg/Kg BW /day) was given to male albino rats, via gavages for 6 days prior exposure to the 1st radiation fraction and the treatment was continued for 14 days after the 1st irradiation fraction till the end of the experiment (4 Gy / week up to 8 Gy total doses). The samples were taken from the blood and some organs, liver and kidney for the biochemical analysis. In the irradiated group, there were a significant decrease in RBCs, WBCs count and Hb content. Dramatic increments in the serum indices of liver (aspartate transaminase, alanine transaminase, alkaline phosphatase and bilirubin) and kidney (urea, uric acid and creatinine) functions were also recorded depicting a liver and kidney impairment state. Also, a significant increase in thiobarbituric acid reactive substances (TBARS) content and Xanthine oxidase (XO) activity in parallel to a significant decrease in the activity of xanthine dehydrogenase accompanied by a significant decrease in reduced glutathione content (GSH), superoxide dismutase (SOD), catalase (CAT) activities were recorded in both liver and kidney tissues compared to control group. Treatment with CP (250 mg/kg) was found to offer significant protection against gamma-radiation induced toxicity in the tissues, which was evident by the improved status of most of the parameters investigated. These results suggest that CP could increase the antioxidant defense systems in the liver and kidney of irradiated animals, and may protect from adverse effects of whole body radiation

  4. Origin and evolution of female plant from an identical male plant, in carica papaya

    International Nuclear Information System (INIS)

    A field study was carried during January 2011 to March, 2013, to confirm the origin and evolution of female plant from an identical male plant in, a dioecious plant, the Carica papaya L. The plants were grown from the seeds of a normal female plant fruit. The grown, plants were identified as XX, XY and XYh (in March - April, 2012) on the basis of male and female flower bearing. The identical male plants, which usually bear only male (unisexual) flowers having calyx, corrolla and androecium, were observed also to bear bisexual flower, having calyx, corrolla, and gynoecium (ovary fused with androecium ). The fruits were set having the bisexual flowers in the identical male (hermaphrodite) plant. These fruits were kept under observation from setting to ripening stage. The ripened fruits were harvested from the identical male plants and 90-95% fruits from these plants were found with the seeds. Plants grown from these male fruit seeds produced all three type of plants i.e., male, female and hermaphrodite. This study indicated that an identical male (XYh) plant produced the female (XX) plant naturally, because of the XXY= XYh condition, which can contribute basic genetic material to male and female plants i.e an identical male (XYh = XXY= 2N +1 = 18+1= 19) produced all three type of plants, the pure male, the hermaphrodite and the female plant, originated from a single source of an identical male, as shown here. XYh = XXY g XY + XX + XXY. The propagation of all three sexes of Carica papaya from a single source of an identical male plant seeds is the first report in the world. (author)

  5. Lactogenic Activity of an Enzymatic Hydrolysate from Octopus vulgaris and Carica papaya in SD Rats.

    Science.gov (United States)

    Cai, Bingna; Chen, Hua; Sun, Han; Sun, Huili; Wan, Peng; Chen, Deke; Pan, Jianyu

    2015-11-01

    The traditional Chinese medicine theory believes that octopus papaya soup can stimulate milk production in lactating women. The objective of this study was to determine whether dietary supplementation with an enzymatic hydrolysate of Octopus vulgaris and Carica papaya (EHOC) could increase milk production and nutritional indexes in Sprague Dawley (SD) rats. Female SD rats (n = 24) were fed a control diet (n = 8), EHOC-supplemented diet, or a positive control diet (Shengruzhi) from day 10 of pregnancy to day 10 of lactation. Maternal serum, mammary gland (day 10 of lactation), milk, and pup weight (daily) were collected for analysis. Results showed that the EHOC diet obviously elevated daily milk yield and pup weight compared to the control group (P < .05). The EHOC diet was found to increase the concentration of prolactin (PRL), progesterone (P), estradiol (E2), and growth hormone (GH) significantly in the circulation and mammary gland. Mammary glands of EHOC-treated dams showed clear lobuloalveolar development and proliferation of myoepithelial cells, but no striking variations were observed among the groups. Furthermore, the nutrition content and immune globulin concentration in the milk of EHOC-supplemented dams were higher than those of the control group, especially the cholesterol, glucose, and IgG were higher by 44.98% (P < .001), 42.76% (P < .01), and 42.23% (P < .01), respectively. In conclusion, this article demonstrates that EHOC administration has beneficial effects on milk production in the dams and on performance of the dam and pup. These results indicate that EHOC could be explored as a potentially lactogenic nutriment for lactating women. PMID:26270883

  6. DEL MALTRATO AL UXORICIDIO. LA VIOLENCIA «PUERTAS ADENTRO» EN LA ALDEA CHILENA TRADICIONAL (Siglo XIX

    Directory of Open Access Journals (Sweden)

    René Salinas Meza

    2011-10-01

    Full Text Available Este artículo pone de manifiesto el contexto en el cual las mujeres chilenas de las aldeas del siglo XIX son víctimas de diversos tipos de agresiones físicas, directas e indirectas, y psicológicas. Las conductas violentas de los agresores son explicadas en el marco de una comunidad que las reconoce como un componente habitual de la vida cotidiana. Así, se delimitan espacios específicos en los que se desenvuelven las relaciones de la gente, tales como el mundo urbano de la aldea y el mundo doméstico de la casa. En ambos, las personas construyen redes colectivas de solidaridad y fraternidad; pero, al mismo tiempo, desarrollan relaciones de competencia que son generadoras de roces y agresiones que ilustran la sociabilidad y el parentesco. Por otra parte, las mujeres agredidas confundieron a menudo la agresión con la aceptación de la autoridad masculina que permitía suponer la existencia de un matrimonio socialmente aceptado y cotidianamente bien llevado. La actitud de rebeldía femenina chocaba con el rechazo social y el ejercicio de un poder marital que se expresaba en conductas agresivas. Así, las relaciones en la pareja no fueron entre iguales, sino jerárquicas, imponiendo a las mujeres un rol subordinado y dependiente frente a los hombres, los que, a su vez, ostentaban la autoridad suficiente para controlarlas y castigarlas. Con la ayuda documental de un abundante número de expedientes judiciales se intenta comprender y explicar las conductas que asumen hombres y mujeres en la relación familiar desarrollada en las aldeas chilenas del siglo XIX.

  7. EL ASESINATO DE HCTOR BARRETO Y LA CULTURA POLTICA DE LA IZQUIERDA CHILENA EN LA DCADA DE 1930

    Scientific Electronic Library Online (English)

    Fabio, Moraga Valle.

    Full Text Available El artculo investiga la formacin de la cultura poltica de la izquierda chilena en su etapa de formacin, durante la dcada de 1930, a travs de la lucha contra el fascismo y el nacismo local, ejemplificado en la muerte del joven cuentista Hctor Barreto. Promisorio escritor, heredero de la cultur [...] a literaria e intelectual de la izquierda chilena de la dcada anterior. Haba entrado a las filas del Partido Socialista en medio de la lucha contra el fascismo desarrollada entre las Milicias Socialistas y las Tropas Nacis de Asalto de Movimiento Nacional Socialista comandado por Jorge Gonzlez von Mares. Su asesinato adquiri en la poca, caractersticas mticas y produjo un giro en un sector importante de la intelectualidad local, la surrealista generacin de 1938, que a partir de entonces se volvi militantemente antifascista y pro socialista. A su vez le dio un hroe al recin formado Partido Socialista, que dos aos despus form parte del gobierno del Frente Popular presidido por Pedro Aguirre Cerda. Abstract in english This article focuses on the political culture of the Chilean left in its first years, the 1930s, during the struggle against local Fascism and Nazism. This battle became legendary following the death of the young writer Hctor Barreto. A promising writer steeped in the literary and intellectual cult [...] ure of the Chilean left of the previous decade, he had entered the ranks of the Socialist Party in the midst of the Socialist Militias struggle against the fascism imposed by the Nazi Shock Troops of the National Socialist Movement commanded by Jorge Gonzalez von Mares. Barretos assassination took on mythical proportions and produced a shift among an important sector of the local intellectuals, the 1938 Surrealist generation, which, from that moment on, became militantly anti-fascist and pro-socialist. At the same time, it created a hero for the recently formed Socialist Party of Chile, which played a role in Pedro Aguirre Cerdas Popular Front government that year.

  8. Conocimientos y autoeficacia asociados a la prevención del VIH y SIDA en mujeres chilenas

    Science.gov (United States)

    Villegas Rodríguez, Natalia; Ferrer Lagunas, Lilian Marcela; Cianelli Acosta, Rosina; Miner, Sarah; Lara Campos, Loreto; Peragallo, Nilda

    2014-01-01

    Resumen Objetivo Evaluar la relación existente entre conocimientos y autoeficacia asociados al VIH/SIDA en mujeres chilenas en desventaja social. Metodología Estudio correlacional, que utiliza la medición basal del estudio “Testeando una intervención en VIH y SIDA en mujeres chilenas”, realizada entre 2006 y 2008, que tiene una muestra de 496 mujeres entre 18 y 49 años residentes en dos comunas de Santiago de Chile. Las participantes respondieron un cuestionario estructurado aplicado por entrevistadoras entrenadas. Este cuestionario incluyó preguntas sobre datos sociodemográficos, escala de conocimientos de conductas de riesgo y autoeficacia, entre otros. Resultados Edad promedio de 32.3±9.1 años, 72.2% vive con su pareja y 42.7% poseen educación media completa. La puntuación media de los conocimientos de la infección por el VIH fue de 8.9±2.5, mientras que para las tres escalas empleadas para medir autoeficacia fueron: “Normas de los pares” =9.8±3.6, “Intención de reducir conductas de riesgo” =12.2±3.6 y “Self Efficacy Form”=20.2±4.7. Los conocimientos tuvieron una correlación positiva débil con la “intención de reducir conductas de riesgo” (r=0.19; pautoeficacia en mujeres chilenas en desventaja social. PMID:25284914

  9. Metáforas en Lengua de Señas Chilena Metaphors in Chilean Sign Language

    Directory of Open Access Journals (Sweden)

    Carolina Becerra

    2008-05-01

    Full Text Available Este estudio describe las características del lenguaje metafórico de personas sordas chilenas y su impacto en la comprensión lingüística. La relevancia de esta pregunta radica en la escasez de investigaciones realizadas, particularmente a nivel nacional. Se desarrolló un estudio cualitativo en base a análisis de videos de sujetos sordos en habla espontánea. Se confeccionó una lista de metáforas conceptuales y no conceptuales en Lengua de Señas Chilena. Posteriormente se evaluó su comprensión en un grupo de sujetos sordos, educados con modalidad comunicativa de lengua de señas. Los resultados obtenidos permiten observar la existencia de metáforas propias de la cultura sorda. Ellas serían coherentes con las particulares experiencias de los sujetos sordos y no necesariamente concuerdan con el lenguaje oral.The present study examined the characteristics of Chilean deaf people's metaphoric language and its relevance in linguistic comprehension. This key question is based in the scarcity of studies conducted in Chile. A qualitative study was developed, on the basis of analysis of videos of Chilean deaf people spontaneous sign language. A list of conceptual and no conceptual metaphors in Chilean sign language was developed. The comprehension of these metaphors was evaluated in a group of deaf subjets, educated using sign language communication. The results identify the existence of metaphors of the deaf culture. These methaphors would be coherent with the particular experiences of deaf subjets and do not necessarily agree with spoken language.

  10. Effect of irradiation on the biochemical and organoleptic changes during the ripening of papaya and mango fruits

    Science.gov (United States)

    Lacroix, Monique; Bernard, Linda; Jobin, Michele; Milot, Sylvain; Gagnon, Marcel

    Papaya and mango rot caused by fungi is a major problem during storage and marketing. Gamma irradiation treatment was used to determine its effect on the quality of papayas and mangoes irradiated at 0,5 to 0,95 kGy. The level of respiration, soluble solids, texture, vitamin C and the sensorial evaluation were effectuated. The results indicate that irradiation treatment reduces significantly (p ⪕ 0,001) the level of respiration and significantly (p ⪕ 0,001) weakens the texture of mangoes. The content of soluble solids and vitamin C are not significantly affected by the irradiation. The sensory evaluation indicates that up to 0,95 kGy the sensorial quality is not changed.

  11. PREVALENCIA DE PROCEDIMIENTOS INVASIVOS EN UNA POBLACIN CHILENA USUARIA DE MTODOS DE CRIBADO Y DIAGNSTICO PRENATAL

    Directory of Open Access Journals (Sweden)

    Mauro Parra C

    2007-01-01

    Full Text Available Objetivo: Evaluar la prevalence del uso de procedimientos invasivos prenatales en una poblacin chilena con cribado y diagnstico de alteraciones morfolgicas y/o cromosmicas. Mtodo: Estudio descriptivo en 17.906 embarazadas entre junio de 2000 y octubre de 2006, a las cuales se les realiz cribado ecogrtico de aneuploidia y malformaciones congnitas durante el primer y segundo trimestre. Las pacientes fueron informadas y aconsejadas acerca de las implicancias de dichos procedimientos. Los procedimientos efectuados fueron la biopsia de vellosidades coriales (BVC, amniocentesis (AMCT y cordocentesis. Se calcul la prevalencia y las indicaciones de dichos procedimientos. Resultados:Se realizaron 81 procedimientos invasivos (0,45%. Las BVC representaron un 43,3%, las AMCT el 37,0% y las cordocentesis un 19,7%. Las principales motivaciones para realizarse un estudio invasivo fueron malformaciones congnitas (48,1 % y aumento del riesgo de aneuploidia (40,7%. Los cariotipos alterados fueron 43,2% del total de los procedimientos, encontrndose 29% de trisomas 21, 46% de trisomas 18 y 13 y 20% de sndrome de Turner. Conclusin: La prevalencia de procedimientos invasivos fue 10 veces inferior a la descrita en pases donde el aborto es legal, y aproximadamente la mitad de ellos resultaron alterados. En nuestra poblacin utilizando una asesora adecuada, la principal motivacin para realizarse un procedimiento invasivo fue el mejor manejo obsttrico del embarazo y, secundariamente, la posibilidad de contar con una mejor informacin sobre la salud del hijo no nacidoObjective: The purpose of this study was to evaluate the prevalence and indications of invasive procedures in a population without termination of pregnancy. Method: This descriptive study was performed in pregnant women who had either assessment of risk for chromosomal abnormalities during the first and second trimester of pregnancy or detection of soft markers or structural defects at any gestational age from June 2000 to October 2006. The patients were counseled with regards their estimated risk, and were informed about invasive diagnostic tests. The invasive testing offered were chorion villous sampling (CVS, amniocentesis (AMCT and fetal blood sampling (FBS. The prevalence of invasive testing was calculated and its causes analyzed according to the type of screening test. Results: Ultrasound was carried out in 17,906 pregnant women, being 70% performed either during the first or second trimester of pregnancy. Eighty one invasive procedures were performed (0.45%. CVS accounted for 43.3%, AMCT for 37.0% and FBS for 19.7%. The main motivation for opting for invasive testing was fetal abnormalities (48.1 % and increased risk of chromosomal anormalities (40.7%. Abnormal fetal kaiyotypes were 43.2%, being 29% Down's syndrome, 46% Edwards and Pateau's syndromes, and 20% Turner syndrome. Conclusion:Jhe low frequency of invasive procedures observed in this population might be an expression of our social, cultural and legal characteristics, but it is also a demonstration of pragmatic and ethically rational decisions about invasive testing for chromosomal abnormalities

  12. Acute and Chronic Hepatotoxicity Study of Orally Administered Ethanol Extract of Carica papaya Seeds in Adult Wistar Rats

    Directory of Open Access Journals (Sweden)

    Wilson O. Hamman

    Full Text Available Carica papaya seed extracts have shown great promise in the quest for the development of natural plant based male contraceptive. This study investigates the acute and chronic hepatotoxicity of ethanol extract of C. papaya seeds in adult Wistar rats. The OECD up-and-down method was used in the acute oral toxicity test using 5 nulliparous, nonpregnant female wistar rats weighing between 180 and 200 g and observed for 14 days. The chronic hepatotoxicity study involved the use of 15 male wistar rats which were divided into 3 groups of 5 each. Group I which served as control were orally administered distilled water while groups II and III received 100 and 250 mg/kg/day of ethanol extract of C. papaya seeds respectively for a period of 90 days. Histological investigation of the liver and serum levels of AST, ALT and ALP were carried out at the end of the administration. Result obtained for the acute oral toxicity showed no mortality or morbidity. There was no significant loss of fur and skin lesions, nose and eyes appeared clear and normal. There was no diarrhea, convulsion, salivation, tremors, lethargy, sleep or coma and animals did not show any sign of aggression or unusual behavior during handling. For the chronic hepatotoxicity study, the histology of the liver, hematological indices and serum levels of AST, ALT and ALP showed no significant difference between the control and experimental groups. The study thus concludes that ethanol extract of C. papaya seeds is non-toxic and safe.

  13. Preliminary In Vitro Antisickilng Properties of Crude Juice Extracts of Persia Americana, Citrus Sinensis, Carica Papaya and Ciklavit®

    OpenAIRE

    Iweala, EE J; Uhegbu, FO; Ogu, GN

    2009-01-01

    The antisickling properties of crude juice extracts of the edible portions of three commonly consumed tropical fruits namely Persia americana, Citrus sinensis, and Carica papaya were investigated in vitro alongside a new drug preparation called Ciklavit® that has antisickling activity. Four different solvent extracts of the crude juice of each fruit including aqueous, acidic, alkaline and alcoholic extracts were prepared and their antisickling effects on sickle cell trait (HbAS) and sickle ce...

  14. Staphylococcal enterotoxin A gene-carrying Staphylococcus aureus isolated from foods and its control by crude alkaloid from papaya leaves.

    Science.gov (United States)

    Handayani, Lita; Faridah, Didah Nur; Kusumaningrum, Harsi D

    2014-11-01

    Staphylococcus aureus is a known pathogen causing intoxication by producing enterotoxins in food. Staphylococcal enterotoxin A is one of the enterotoxins commonly implicated in staphylococcal food poisoning. The ability of crude alkaloid extract from papaya leaves to inhibit the growth of S. aureus and staphylococcal enterotoxin A synthesis was investigated. Staphylococcal enterotoxin A gene-carrying S. aureus was isolated from raw milk and ready-to-eat foods. Crude alkaloid was extracted from ground, dried papaya leaves using ultrasonic-assisted extraction, and a MIC of the alkaloid was determined by the broth macrodilution method. Furthermore, S. aureus isolate was exposed to the crude alkaloid extract at one- and twofold MIC, and the expression of sea was subsequently analyzed using a quantitative reverse transcription real-time PCR. Ten isolates of S. aureus were obtained, and nine of those isolates were sea carriers. The yield of crude alkaloid extract was 0.48 to 1.82% per dry weight of papaya leaves. A MIC of crude alkaloid to S. aureus was 0.25 mg/ml. After exposure to the alkaloid at 0.25 and 0.5 mg/ml for 2 h, a significant increase in cycle threshold values of sea was observed. The sea was expressed 29 and 41 times less when S. aureus was exposed to crude alkaloid at one- and twofold MIC, respectively. This study revealed that crude alkaloid of papaya leaves could control staphylococcal enterotoxin A gene-carrying S. aureus by suppressing the expression of sea, in addition to the ability to inhibit the growth of S. aureus. The expression of sea was successfully quantified. PMID:25364936

  15. Antihyperglycemic and hypolipidemic activities of aqueous extract of Carica papaya Linn. leaves in alloxan-induced diabetic rats

    OpenAIRE

    Yasmeen Maniyar; Prabhu Bhixavatimath

    2012-01-01

    Background: India is considered as the diabetic capital of the world. The study of plants having antihyperglycemic and hypolipidemic activities may give a new approach in the treatment of diabetes mellitus. Objective: The study was intended to evaluate the antihyperglycemic and hypolipidemic activity of aqueous extract of leaves of Carica papaya Linn. (AECPL) in alloxan-induced diabetic albino rats. Materials and Methods: Diabetes was induced in albino rats by administration of alloxan monohy...

  16. Glomus intraradices Attenuates the Negative Effect of Low Pi Supply on Photosynthesis and Growth of Papaya Maradol Plants

    OpenAIRE

    Nava-Gutiérrez Yolanda; Ronald Ferrera-Cerrato; Jorge M. Santamaría

    2012-01-01

    Low inorganic phosphorus (Pi) supply limits the photosynthetic process and hence plants growth and development. Contradictory reports exist in the literature on whether mycorrhyzal association can attenuate the negative effects of low Pi supply on photosynthesis and growth. In the present paper, the effect that low Pi supply may have on photosynthesis and growth of papaya Maradol plants was evaluated in intact plants and in those inoculated with two different strains of the arbuscular mycorrh...

  17. La expansi?n internacional de la industria vitivin?cola chilena. Estudio emp?rico de los factores y nivel exportador

    OpenAIRE

    Liberman, Sammy; Baena Graciá, Verónica; Moreno, Fátima

    2010-01-01

    [ES] Este trabajo propone una serie de factores como determinantes del nivel de expansión internacional de la industria vitivinícola chilena: i) tamaño de la empresa; ii) gestión de la calidad; iii) tecnologías de la información y la comunicación; iv) planificación de la gestión internacional, y finalmente, v) adaptación de la estrategia de marketing internacional. El contraste empírico sobre la base de datos de exportación de la industria vitivinícola chilena entre los años 2004 y 2007, perm...

  18. The component of Carica papaya seed toxic to A. aegypti and the identification of tegupain, the enzyme that generates it.

    Science.gov (United States)

    Nunes, Natalia N dos S; Santana, Lucimeire A; Sampaio, Misako U; Lemos, Francisco J A; Oliva, Maria Luiza

    2013-07-01

    As Aedes aegypti transmits the etiologic agents of both yellow and dengue fever; vector control is considered essential to minimise their incidence. The aim of this work was to identify the component of Carica papaya seed toxic to A. aegypti, and the identification of tegupain, the enzyme that generates it. Aqueous extracts (1%, w/v) of the seed tegument and cotyledon of C. papaya are not larvicidal isolately. However, a mixture of 17μgmL(-1) tegument extract and 27μgmL(-1) cotyledon extract caused 100% larval mortality in a bioassay. The mixture was no longer larvicidal after the tegument extract was pre-treated at 100°C for 10min. The enzyme tegupain efficiently hydrolysed the substrate Z-Phe-Arg-pNan (Km 58.8μM, Kcat 28020s(-1), Kcat/Km 5×10(8)M(-1) s(-1)), and its activity increased with 2mM dithiothreitol (DTT), at 37°C, pH 5.0. The chelating agent EDTA did not modify the enzyme activity. Inhibition of tegupain by cystatin (Kiapp 2.43nM), E64 (3.64nM, 83% inhibition), and the propeptide N-terminal sequence indicate that the toxic activity is due to a novel cysteine proteinase-like enzyme, rendered active upon the hydrolysis of a cotyledon component of C. papaya seeds. PMID:23402920

  19. Effect of Fitomas-E on seedling production of papaya var. Maradol Roja in Cienfuegos,Cuba

    Directory of Open Access Journals (Sweden)

    Fernando G. Serbelló Guzmán

    2016-02-01

    Full Text Available An experiment in nursery in bag conditions was carried out at the Cienfuegos Horticultural Company, in order to evaluate the effect of different alternatives to stimulate the growth and the development of papaya seedlings (Carica papaya L. variety Maradol Red, in the period from June to September 2010. The treatments were: applying Fitomas-E, Trichoderma, EcoMic, the combination of Trichoderma + EcoMic and a control without application. We evaluated the height and diameter at four different positions, the number of leaves, green and dry mass of roots as well as for the external part of the plant at the transplantation time. The obtained data were subjected to the given statistical analyzes. Results indicate that the height and diameter of the papaya plants are increased with the application of Fitomas E and EcoMic, while a higher number of leaves is reached with the application of Fitomas E, and the combination of EcoMic with Trichoderma.

  20. Crystallization and preliminary X-ray analysis of a protease inhibitor from the latex of Carica papaya

    International Nuclear Information System (INIS)

    The Kunitz-type trypsin/chymotrypsin inhibitor isolated from C. papaya latex has been crystallized using the hanging-drop vapour-diffusion method. Two different crystal forms are observed, diffracting to 2.6 and 1.7 Å. A Kunitz-type protease inhibitor purified from the latex of green papaya (Carica papaya) fruits was crystallized in the presence and absence of divalent metal ions. Crystal form I, which is devoid of divalent cations, diffracts to a resolution of 2.6 Å and belongs to space group P31 or P32. This crystal form is a merohedral twin with two molecules in the asymmetric unit and unit-cell parameters a = b = 74.70, c = 78.97 Å. Crystal form II, which was grown in the presence of Co2+, diffracts to a resolution of 1.7 Å and belongs to space group P212121, with unit-cell parameters a = 44.26, b = 81.99, c = 140.89 Å

  1. Current status of tropical fruit breeding and genetics for three tropical fruit species cultivated in Japan: pineapple, mango, and papaya.

    Science.gov (United States)

    Ogata, Tatsushi; Yamanaka, Shinsuke; Shoda, Moriyuki; Urasaki, Naoya; Yamamoto, Toshiya

    2016-01-01

    Tropical fruit crops are predominantly produced in tropical and subtropical developing countries, but some are now grown in southern Japan. Pineapple (Ananas comosus), mango (Mangifera indica) and papaya (Carica papaya) are major tropical fruits cultivated in Japan. Modern, well-organized breeding systems have not yet been developed for most tropical fruit species. Most parts of Japan are in the temperate climate zone, but some southern areas such as the Ryukyu Islands, which stretch from Kyushu to Taiwan, are at the northern limits for tropical fruit production without artificial heating. In this review, we describe the current status of tropical fruit breeding, genetics, genomics, and biotechnology of three main tropical fruits (pineapple, mango, and papaya) that are cultivated and consumed in Japan. More than ten new elite cultivars of pineapple have been released with improved fruit quality and suitability for consumption as fresh fruit. New challenges and perspectives for obtaining high fruit quality are discussed in the context of breeding programs for pineapple. PMID:27069392

  2. Crystallization and preliminary X-ray analysis of a protease inhibitor from the latex of Carica papaya

    Energy Technology Data Exchange (ETDEWEB)

    Azarkan, Mohamed [Université Libre de Bruxelles, Faculty of Medicine, Protein Chemistry Unit, Campus Erasme (CP 609), 808 Route de Lennik, B-1070 Brussels (Belgium); Garcia-Pino, Abel [Department of Molecular and Cellular Interactions, Vlaams Interuniversitair Instituut voor Biotechnologie and Laboratorium voor Ultrastructuur, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussel (Belgium); Dibiani, Rachid [Université Libre de Bruxelles, Faculty of Medicine, Protein Chemistry Unit, Campus Erasme (CP 609), 808 Route de Lennik, B-1070 Brussels (Belgium); Wyns, Lode; Loris, Remy, E-mail: reloris@vub.ac.be [Department of Molecular and Cellular Interactions, Vlaams Interuniversitair Instituut voor Biotechnologie and Laboratorium voor Ultrastructuur, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussel (Belgium); Baeyens-Volant, Danielle [Université Libre de Bruxelles, Faculty of Medicine, Protein Chemistry Unit, Campus Erasme (CP 609), 808 Route de Lennik, B-1070 Brussels (Belgium)

    2006-12-01

    The Kunitz-type trypsin/chymotrypsin inhibitor isolated from C. papaya latex has been crystallized using the hanging-drop vapour-diffusion method. Two different crystal forms are observed, diffracting to 2.6 and 1.7 Å. A Kunitz-type protease inhibitor purified from the latex of green papaya (Carica papaya) fruits was crystallized in the presence and absence of divalent metal ions. Crystal form I, which is devoid of divalent cations, diffracts to a resolution of 2.6 Å and belongs to space group P3{sub 1} or P3{sub 2}. This crystal form is a merohedral twin with two molecules in the asymmetric unit and unit-cell parameters a = b = 74.70, c = 78.97 Å. Crystal form II, which was grown in the presence of Co{sup 2+}, diffracts to a resolution of 1.7 Å and belongs to space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 44.26, b = 81.99, c = 140.89 Å.

  3. Efecto de la madurez, geometría y presión sobre la cinética de transferencia de masa en la deshidratación osmótica de papaya (Carica papaya L., var. Maradol

    Directory of Open Access Journals (Sweden)

    Lina Marcela Chavarro-Castrillón

    2006-09-01

    Full Text Available Las papayas obtenidas en el raleo (papayas menos desarrolladas entresacadas de los árboles para beneficiar el crecimiento de las otras generalmente se descartan. Alternativamente, estas papayas pueden secarse por un proceso de deshidratación osmótica y secado térmico convencional para usarlas como snacks o como ingrediente para otros productos. Se comparó la cinética de transferencia de masa en la deshidratación osmótica de papayas de raleo frente a papayas desarrolladas con diferente grado de madurez, considerando el efecto de la geometría de la muestra y de la presión del proceso. La deshidratación osmótica se efectuó en una solución agitada de sacarosa a 50 °Brix, a 25 °C. Se consideraron tres niveles de madurez: raleo, verde y pintona, tres geometrías: lámina, cilindro y anillo y dos niveles de presión: atmosférica y vacío. Se estudiaron como variables cinéticas la variación de peso (WR, pérdida de agua (WL y ganancia de sólidos (SG entre 10 y 180 min. La madurez tuvo efecto significativo sobre las tres respuestas de la cinética a 30 min y sobre SG a 180 min; la geometría tuvo efecto sobre WR y SG a 30 min y sobre los tres parámetros cinéticos a 180 min y la presión solamente tuvo efecto sobre WL y SG a 30 min. La mayor pérdida de agua (65% se obtuvo a 30 min para la combinación raleo/cilindro/vacío; mientras que la mayor ganancia de sólidos fue 31% para el tratamiento verde/lámina/vacío a 180 min.

  4. Artificial neural network analysis of genetic diversity in Carica papaya L. / Rede neural artificial na anlise da divergncia gentica em Carica papaya L.

    Scientific Electronic Library Online (English)

    Cibelle Degel, Barbosa; Alexandre Pio, Viana; Silvana Silva Red, Quintal; Messias Gonzaga, Pereira.

    2011-09-01

    Full Text Available O estudo da diversidade gentica de fundamental importncia na seleo preliminar de acessos com caractersticas superiores e a utilizao desses materiais com sucesso em programas de melhoramento gentico. O objetivo deste trabalho foi avaliar, como estratgia de anlise da diversidade gentica, [...] a tcnica de bioinformtica denominada rede neural artificial. Foi considerada a mdia de trs pocas de plantio, oito caracteres quantitativos e trinta e sete acessos de mamoeiro, utilizando-se o delineamento em blocos casualizados com duas repeties. Com base na anlise discriminante de Anderson, 91,90 % dos acessos foram classificados corretamente nos grupos previamente definidos pela rede neural artificial. Concluiu-se que a tcnica de rede neural artificial se demonstrou vivel na classificao dos acessos. Observou-se a presena significativa de diversidade gentica entre os acessos avaliados. Abstract in english The study of genetic diversity is fundamental in the preliminary selection of accessions with superior characteristics and for a successful use of these genotypes in breeding programs. The purpose of this study was to evaluate, as a strategy for genetic diversity analysis, the bioinformatics approac [...] h called artificial neural network. Based on the average of three growing seasons, eight quantitative traits and thirty-seven papaya accessions were evaluated in a randomized complete block design, with two replications. By Anderson's discriminant analysis, 91.90 % of the accessions were correctly classified in the groups previously defined by artificial neural network. It was concluded that the technique of artificial neural network is feasible to classify the accessions. The presence of significant genetic diversity among accessions was observed.

  5. Características fenotípicas y agronómicas de seis genotipos de papaya (Carica papaya L. de Tuxpan, Guerrero, México

    Directory of Open Access Journals (Sweden)

    José Ángel Alcántara Jiménez

    2010-06-01

    Full Text Available En México la producción de papayo (Carica papaya L. es afectada por factores genéticos, ambientales y bióticos. Destacan los daños provocados por el virus de la mancha anular, el cual reduce considerablemente la cantidad y calidad de la fruta; por eso es muy importante, conocer la productividad de los genotipos en un ambiente determinado. La presente investigación se realizó con el objetivo de evaluar el desarrollo y las características agronómicas de seis genotipos de papayo. El estudio se llevó a cabo en la localidad de Tuxpan, Guerrero, donde se cultivaron los genotipos: R4M3, Maradol, Criolla, Red Lady, Zapote y R5M2. Como diseño experimental se utilizó el de bloques completos al azar, con cuatro repeticiones. Las variables evaluadas fueron: altura al primer fruto, diámetro del cuello, altura de la planta, número de días a la cosecha, rendimiento de frutos (t/ha, número, diámetro y longitud de frutos, concentración de azúcares (grados Brix, textura, color de la pulpa y epicarpio de frutos maduros. Los datos de las variables se sometieron a análisis de varianza y prueba de comparación de medias de Tukey. En relación a la adaptación de los genotipos: todos los genotipos se desarrollaron y produjeron frutos en las condiciones de trópico seco. La variedad Maradol fue la más sobresaliente, presentando un porte menor, mayor precocidad y productividad, textura dura de pulpa, alta concentración de azúcares, buen tamaño y color.

  6. Actividad antifngica del aceite esencial de naranja (Citrus sinensis L.) sobre hongos postcosecha en frutos de lechosa (Carica papaya L.) / Anti-fungal activity of essential orange oil (Citrus sinensis L.) over post-harvest fungi in papaya fruits (Carica papaya L.)

    Scientific Electronic Library Online (English)

    Clemencia, Gudez; Luis, Caizalez; Laura, Avendao; Jos, Scorza; Carmen, Castillo; Rafael, Olivar; Yolanda, Mndez; Libert, Snchez.

    2014-12-01

    Full Text Available Los hongos postcosecha causan pudriciones que afectan la calidad de los frutos de lechosa (Carica papaya L.). Actualmente los aceites esenciales son considerados una alternativa a los fungicidas qumicos para controlar estos hongos. En este estudio se evalu la actividad antifngica del aceite esenc [...] ial de naranja (Citrus sinensis L.) sobre los hongos Colletotrichum gloeosporioides, Penicillium indicum, Fusarium solani, Rhizopus stolonifer y Aspergillus flavus, en medio de cultivo Papa Dextrosa Agar (PDA) en un diseo completamente al azar. Los resultados mostraron que existe un efecto inhibidor del aceite esencial de naranja (AEN) en el crecimiento micelial de los hongos in vitro, superior al 80% a concentracin de AE de 1%, y 100% de inhibicin a concentraciones de 2,5% y 5% de AE (p Abstract in english Post-harvest fungi produce rotting that affects the quality of papaya fruits (Carica papaya L.) At present, essential oils are considered an alternative to chemical anti-fungal substances for controlling these fungi. In this study we evaluated the antifungal activity of essential orange oil (Citrus [...] sinensis L.) over Colletotrichum gloeosporioides, Penicillum indicum, Fusarium solani, Rhizopus stolonifer and Aspergillus flavus, in Potato Dextrose Agar (PDA) culture medium in a completely random design. The results showed that there is an inhibitory effect of essential orange oil (EOO) over the in vitro mycelium growth of fungi, higher than 80% at a 1% EOO concentration and 100% inhibition at 2.5% and 5% EOO concentrations (p

  7. Uso de extratos vegetais no controle in vitro do Colletotrichum gloeosporioides penz. Coletado em frutos de mamoeiro (Carica papaya L.) / Use of plant extracts on control in vitro of Colletotrichum gloeosporioides penz. Collected in papaya fruits (Carica papaya L.)

    Scientific Electronic Library Online (English)

    Elzivan Felix, Ferreira; Abel Rebouas, So Jos; Marins Pereira, Bomfim; John Silva, Porto; Jailson Silva de, Jesus.

    2014-06-01

    Full Text Available Diversas alternativas vm sendo estudadas e utilizadas no controle de doenas de plantas, no intuito de suprir as necessidades dos produtores e consumidores no desejo de reduzir o uso de defensivos agrcolas. Assim, o objetivo deste trabalho foi avaliar a atividade de extratos vegetais de Azadiracht [...] a indica A. Juss., Anonna muricata L. e de Lippia alba (Mill) N. E. Brown. no controle de Colletotrichum gloeosporioides Penz. in vitro. O experimento foi conduzido na Universidade Estadual do Sudoeste da Bahia, Cmpus de Vitria da Conquista, Bahia, Brasil. Extratos aquosos de folhas de graviola e erva-cidreira, e extratos aquosos de sementes de nim e graviola foram usados visando inibio do crescimento micelial do patgeno causador da antracnose em frutos de mamo. A atividade antifngica dos extratos foi mensurada mediante a medio do crescimento micelial das culturas. O delineamento adotado foi o DIC em esquema fatorial (3x3x3), com quatro repeties, nos dois experimentos. Os resultados obtidos indicam maior inibio ao crescimento micelial do patgeno com o uso dos extratos de folhas de erva-cidreira e de sementes de graviola. Abstract in english Several alternatives have been studied and used in the control of plant diseases, in order to achieve the needs of producers and consumers desiring to reduce the use of pesticides. Thus, the objective of this study was evaluating the activity of plant extracts Azadirachta indica A Juss., Annona muri [...] cata L. and Lippia alba (Mill) N. E. Brown. in controlling of Colletotrichum gloeosporioides Penz. in vitro. The experiment was conducted in State University of Southwest Bahia, campus of Vitoria da Conquista city, State of Bahia, Brazil. Aqueous extracts of leaves of soursop leaves and lemongrass, and aqueous extracts of neem seeds and soursop were used to inhibit mycelial growth of pathogen of anthracnose from papaya fruits. The antifungal activity of the extracts was determined by measuring the mycelial growth of cutures. The experimental design was CRD in factorial (3x3x3) with four repetitions in both experiments. The results suggested inhibitory action of the leaf extract of lemongrass and the seed extract of soursop.

  8. Metabolomic profiling of the phytomedicinal constituents of Carica papaya L. leaves and seeds by 1H NMR spectroscopy and multivariate statistical analysis.

    Science.gov (United States)

    Gogna, Navdeep; Hamid, Neda; Dorai, Kavita

    2015-11-10

    Extracts from the Carica papaya L. plant are widely reported to contain metabolites with antibacterial, antioxidant and anticancer activity. This study aims to analyze the metabolic profiles of papaya leaves and seeds in order to gain insights into their phytomedicinal constituents. We performed metabolite fingerprinting using 1D and 2D 1H NMR experiments and used multivariate statistical analysis to identify those plant parts that contain the most concentrations of metabolites of phytomedicinal value. Secondary metabolites such as phenyl propanoids, including flavonoids, were found in greater concentrations in the leaves as compared to the seeds. UPLC-ESI-MS verified the presence of significant metabolites in the papaya extracts suggested by the NMR analysis. Interestingly, the concentration of eleven secondary metabolites namely caffeic, cinnamic, chlorogenic, quinic, coumaric, vanillic, and protocatechuic acids, naringenin, hesperidin, rutin, and kaempferol, were higher in young as compared to old papaya leaves. The results of the NMR analysis were corroborated by estimating the total phenolic and flavonoid content of the extracts. Estimation of antioxidant activity in leaves and seed extracts by DPPH and ABTS in-vitro assays and antioxidant capacity in C2C12 cell line also showed that papaya extracts exhibit high antioxidant activity. PMID:26163870

  9. Sorologia e sobrevivncia do vrus do amarelo letal do mamoeiro / Serology and surviving characteristics of Papaya lethal yellowing virus

    Scientific Electronic Library Online (English)

    Ana Lcia Lopes do, Nascimento; Jos Albersio de Araujo, Lima; Aline Kelly Queiroz do, Nascimento; Maria de Ftima Barros, Gonalves.

    2010-09-01

    Full Text Available O vrus do amarelo letal do mamoeiro (Papaya lethal yellowing virus, PLYV) responsvel por uma das principais doenas do mamoeiro (Carica papaya) no Nordeste brasileiro. O PLYV pode ser transmitido atravs do solo, gua, instrumento de corte e mos contaminadas. A presente pesquisa teve como objet [...] ivo estudar as caractersticas biolgicas, sorolgicas e fsicas de um isolado do vrus e avaliar sua sobrevivncia em tecido seco infetado. O PLYV foi detectado por "Enzyme linked immunosorbent assay" (ELISA) indireto e isolado em mudas de mamoeiro, atravs da inoculao mecnica. A sobrevivncia do PLYV em folhas e razes secas de mamoeiro infetado foi avaliada por sorologia e inoculao em plantas sadias. A presena do vrus foi detectada em folhas e razes de mamoeiro secas, indicando que o vrus pode permanecer ativo em restos de cultura por at 120 dias. A purificao do PLYV permitiu a obteno de 309.5 mg de vrus/kg de folha e o anti-soro obtido mostrou-se altamente especfico, com ttulos de 1:128 em dupla difuso em Agar e 1:1.024.000 em Elisa indireto. Estudos das propriedades fsicas do PLYV em mamoeiro revelaram um ponto de inativao trmica (PIT) em torno de 80 C, um ponto mximo de diluio (PMD) de ac. 10-6 e uma longevidade in vitro (LIV) acima de 50 dias. Abstract in english The Papaya lethal yellowing virus (PLYV) is responsible for one of the most important disease of papaya (Carica papaya) in the Northeast of Brazil. The PLYV can be transmitted through the soil, irrigation water, agriculture tools and contaminated hands. The present research had the objective to char [...] acterize biologically, serologically and physically a PLYV isolate and evaluates how long it survives in infected dried tissues. The PLYV was identified by indirect enzyme linked immunoabsorbent assay (ELISA), isolated in young papaya plants by mechanical inoculations and maintained at green house conditions for virus purification. Approximately 309.5 mg of virus was purified per kg of infected papaya leaves and a polyclonal antiserum was obtained from an immunized rabbit. The antiserum obtained was shown to be highly specific to PLYV with a titer of 1:128 in double immune-diffusion and a titer of 1:1.024.000 in indirect ELISA. The virus was detected in dried roots and leaves maintained at room temperature up to 120 days, confirming its high stability within nonliving plant tissues, which could explain its dissemination by contaminated hands, tools, water and soil. The physical properties determined for the virus revealed a thermal inactivation point of 80 C, longevity in vitro over 50 days and dilution end point ac. of 10-6.

  10. Tratamentos para superao da dormncia em sementes de mamo Dormancy overcoming in papaya seeds

    Directory of Open Access Journals (Sweden)

    Da Tokuhisa

    2007-04-01

    Full Text Available O trabalho teve como objetivo definir tratamentos adequados para a superao da dormncia em sementes de mamo. Foram utilizadas sementes com e sem sarcotesta, extradas de frutos do grupo Formosa, hbrido Tainung 01. Nas sementes sem sarcotesta, foram aplicados os seguintes tratamentos: lavagem em gua corrente por 2 e 4 h, pr-secagem a 40C/96h, pr-esfriamento a 10C/14 dias, envelhecimento acelerado a 41C por 36, 48 e 72h, imerso em NaOCl 0,5% por 1, 2, 3, 4 e 5h, imerso em KNO3 1M por 30, 60, 90 e 120 min., imerso em GA3 400, 600 e 800 ppm por 24 h, umedecimento do substrato com GA3 400, 600 e 800 ppm, armazenamento por 3, 6 e 9 meses e choque trmico a 15-35C. Aps cada tratamento, avaliou-se a germinao aos 15 e 30 dias aps a semeadura. A presena da sarcotesta na semente diminui a velocidade e a porcentagem de germinao. Os tratamentos mais eficientes para a superao da dormncia em sementes de mamo so o umedecimento do substrato com GA3 600 ppm ou a imerso das sementes em soluo de GA3 600 ppm por 24 horas e a imerso das sementes em KNO3 1M por 30, 60 e 90 minutos. A dormncia foi superada aps o armazenamento das sementes por 3 a 6 meses.The objective of this study was to define appropriate treatments to overcome dormancy in papaya seeds. Seeds with and without sarcotesta extracted from papaya fruits of the Formosa group, hybrid Tainung 01, were used. The following treatments to overcome dormancy were applied on seeds without sarcotesta: leaching for 2 and 4h, drying at 40C/96h, pre-chilling at 10C/14 days, accerelated aging at 41C for 36, 48 e 72h, soaking in NaOCl 0,5% for 1, 2, 3, 4 and 5h, soaking in KNO3 1M for 30, 60, 90 and 120 min., soaking in GA3 400, 600 and 800 ppm for 24h, substratum moistened with GA3 400, 600 and 800 ppm, storage for 3, 6 and 9 months and heat-shock at 15-35 C. After each treatment, germination at 15 and 30 days was evaluated. Seeds with sarcotesta had lower germination speed and percentage. The best treatments to overcome dormancy in papaya seeds were the use of substratum moistened with GA3 600 ppm or the immersion of the seeds GA3 600 ppm for 24 hours and immersion of the seeds in KNO3 1M for 30, 60 and 90 minutes. Seed dormancy was broken after storage for 3 and 6 months.

  11. Expresso transiente do gene gus, sob regulao de quatro promotores, em diferentes tecidos de mamoeiro (Carica papaya L.) e videira (Vitis sp.) / Transient expression of the gus gene, under the regulation of four promoters, on different tissues of papaya (Carica papaya l.) and grape (Vitis sp.)

    Scientific Electronic Library Online (English)

    Adelar Almeida, Pinto; Marly Catarina Felipe, Coelho; Manoel Teixeira, Souza Jr; Miguel Pedro, Guerra.

    2002-12-01

    Full Text Available O mamoeiro (Carica papaya L.) e a videira (Vitis vinifera L.) destacam-se entre as fruteiras produzidas no Brasil por serem plantadas em quase todo o territrio nacional e apresentarem importncia econmica e social. A tecnologia de produo de organismos geneticamente modificados, tambm conhecidos [...] como "transgnicos", tem grande potencial de uso no desenvolvimento de fruteiras melhoradas. Porm, questes de propriedade intelectual limitam o uso da engenharia gentica por pases em desenvolvimento, que normalmente no detm direitos sobre processos ou produtos necessrios ao uso desta. Neste contexto, o presente estudo buscou avaliar promotores de expresso gnica alternativos ao CaMV 35S, que o mais utilizado no desenvolvimento de transgnicos, mas patenteado. Para tanto, construes gnicas com o gene gus sob a regulao de diferentes promotores foram testadas para expresso transiente em diversos tecidos de mamoeiro e videira. Expresso transiente foi avaliada em embries somticos, folhas, caules, razes e frutos. O promotor do gene UBQ3, que constitutivo e se encontra em domnio publico, mostrou ser uma alternativa promissora para futuros trabalhos de transformao gentica de mamoeiro, mas no de videira. Abstract in english Papaya (Carica papaya L.) and grapes (Vitis vinifera L.) are among the most important fruit crops produced in Brazil, and are cultivated in several regions around the country being of economical and social importance. Genetic engineering has great potential on the development of genetically improved [...] fruit crops. However, intellectual property issues constantly limit the commercial use of this technology in developing countries. Thus, the present study aimed to evaluate gene promoters as alternatives to the 35S CaMV, which is the most used so far in the development of transgenic plants, but it is patented. In order to do that, the expression of the gus gene under the regulation of distinct promoters was evaluated on different papaya and grape tissues. Transient expression was evaluated on somatic embryos, leaves, stems, roots and fruits. The UBQ3 promoter, which is constitutive and is in the public domain, showed to be a real promising alternative for future work on genetic transformation of papaya but not grapes.

  12. Construction of physical maps for the sex-specific regions of papaya sex chromosomes

    Directory of Open Access Journals (Sweden)

    Na Jong-Kuk

    2012-05-01

    Full Text Available Abstract Background Papaya is a major fruit crop in tropical and subtropical regions worldwide. It is trioecious with three sex forms: male, female, and hermaphrodite. Sex determination is controlled by a pair of nascent sex chromosomes with two slightly different Y chromosomes, Y for male and Yh for hermaphrodite. The sex chromosome genotypes are XY (male, XYh (hermaphrodite, and XX (female. The papaya hermaphrodite-specific Yh chromosome region (HSY is pericentromeric and heterochromatic. Physical mapping of HSY and its X counterpart is essential for sequencing these regions and uncovering the early events of sex chromosome evolution and to identify the sex determination genes for crop improvement. Results A reiterate chromosome walking strategy was applied to construct the two physical maps with three bacterial artificial chromosome (BAC libraries. The HSY physical map consists of 68 overlapped BACs on the minimum tiling path, and covers all four HSY-specific Knobs. One gap remained in the region of Knob 1, the only knob structure shared between HSY and X, due to the lack of HSY-specific sequences. This gap was filled on the physical map of the HSY corresponding region in the X chromosome. The X physical map consists of 44 BACs on the minimum tiling path with one gap remaining in the middle, due to the nature of highly repetitive sequences. This gap was filled on the HSY physical map. The borders of the non-recombining HSY were defined genetically by fine mapping using 1460?F2 individuals. The genetically defined HSY spanned approximately 8.5?Mb, whereas its X counterpart extended about 5.4?Mb including a 900 Kb region containing the Knob 1 shared by the HSY and X. The 8.5?Mb HSY corresponds to 4.5?Mb of its X counterpart, showing 4?Mb (89% DNA sequence expansion. Conclusion The 89% increase of DNA sequence in HSY indicates rapid expansion of the Yh chromosome after genetic recombination was suppressed 23 million years ago. The genetically defined borders coincide with the common BACs on the minimum tiling paths of HSY and X. The minimum tiling paths of HSY and its X counterpart are being used for sequencing these X and Yh-specific regions.

  13. Rol del ingeniero civil en la sociedad chilena contempornea / Role of the Civil Engineer in the Contemporary Chilean Society

    Scientific Electronic Library Online (English)

    E, Forcael; S, Vargas; A, Opazo; L, Medina.

    2013-11-01

    Full Text Available A pesar de toda la informacin existente, acerca de los factores que pudieran definir en s un rol para el ingeniero civil de la sociedad chilena contempornea, existe limitada evidencia de un estudio que integre todos estos factores, a travs de un modelo que permita definir este rol, por medio de [...] un anlisis cualitativo y cuantitativo. Este trabajo presenta los resultados obtenidos del anlisis del rol del ingeniero civil en la sociedad chilena contempornea, particularmente desde la creacin en Chile, el ao 1939, de la Corporacin de Fomento de la Produccin (CORFO), hasta nuestros das. El estudio se centra en la obtencin de informacin a travs de una exhaustiva revisin bibliogrfica, junto con la aplicacin de un instrumento de medicin (entrevista), a algunos de los ingenieros civiles ms prestigiosos del pas, tanto del mbito acadmico, como del profesional; informacin que fue posteriormente procesada y analizada, utilizando ecuaciones estructurales. Esto permiti establecer algunos de los parmetros que mejor definen el rol del ingeniero civil contemporneo en Chile, a saber: Formacin Acadmica, Desempeo Profesional, Liderazgo, Innovacin y Vinculacin con el Medio. El anlisis cuantitativo de dichos parmetros se realiz utilizando ecuaciones estructurales, particularmente a travs del uso de Partial Least Square (PLS). Con el fin de complementar y robustecer el anlisis cuantitativo, se realiz adems un anlisis cualitativo, por medio de la tcnica de patrones de ajuste (pattern-matching). As, fue posible construir y validar un modelo que permitiera caracterizar el rol del ingeniero civil contemporneo, en funcin de los parmetros antes mencionados, concluyendo que el papel de este profesional en la sociedad chilena contempornea, se fundamenta en la formacin acadmica que recibe, la creatividad e ingenio que posee para aportar con soluciones innovadoras, el logro de un desempeo profesional de excelencia, y la consideracin en su quehacer del bienestar del medio con el cual se vincula. Abstract in english Despite all existing information about the factors that could define a role for the Civil Engineer of the contemporary Chilean society, there is limited evidence of a study that integrates all these factors, through a model that allows defining this role by using a qualitative and quantitative analy [...] sis. This paper presents the results obtained from the analysis of the role of the Civil Engineer in the contemporary Chilean society, particularly since the creation of the Chilean Economic Development Agency (CORFO) in 1939, until today. The study focuses on obtaining information through a comprehensive literature review, along with the application of an assessment instrument (interview), to some of the most prestigious Civil Engineers in Chile, both professors and/or professionals; information that was then processed and analyzed by using Structural Equation Modeling (SEM). This allowed establishing some of the parameters that best define the role of the contemporary Civil Engineer in Chile, namely: Academic Background, Professional Performance, Leadership, Innovation and, Connection with Society . Quantitative analysis of these parameters was performed by means of Structural Equation Modeling, particularly through the use of Partial Least Square (PLS). In order to complement and strengthen the quantitative analysis, a qualitative analysis was also performed, through the technique of pattern-matching. Thus, it was possible to build and validate a model that would characterize the functions of the contemporary Civil Engineer, based on the aforementioned parameters, concluding that the role of this professional, in the contemporary Chilean society, is based on her/his academic background, the creativity and ingenuity that has to provide innovative solutions, through an excellent professional performance, and taking into account the welfare of the environment where she/he works.

  14. La solidez de la economía chilena a la luz de las reformas estructurales: 1970-1997

    Directory of Open Access Journals (Sweden)

    Ángeles SÁNCHEZ DÍEZ

    2009-11-01

    Full Text Available RESUMEN: En el presente artículo se recogen las principales reformas estructurales que han tenido lugar en Chile desde 1970 y que constituyen la base de la solidez actual de dicha economía latinoamericana. Se propone una división en períodos en la que se distingue la época del gobierno de la Unidad Popular, el gobierno de la Junta Militar y la transición a la democracia para evaluar los resultados económicos que se registraron a la luz de las distintas estrategias de desarrollo. Las principales reformas que se dieron en el periodo 1970-1973 bajo la "vía chilena hacia el socialismo" fueron la creación del Área de Propiedad social, la profundización de la Reforma Agraria y la nacionalización de la Gran Minería Durante el monetarismo ortodoxo (1974-1982 destacan como principales reformas la liberalización y desregulación de los mercados, la l a ronda de privatizaciones, la apertura comercial, la liberalización financiera y las "siete modernizaciones", todas ellas respaldadas por la ortodoxia de la denominada Escuela de Chicago. Tras la profunda crisis de 1982 que afectó a todo el continente latinoamericano fue necesario replantear el modelo y se introdujeron distintas medidas de carácter neoliberal que consiguieron que fuera posible aprovechar los beneficios de las reformas realizadas con anterioridad. La transición a la democracia constituye un reto para la sociedad chilena, en tanto que contando con las bases de una economía sólida deben incorporarse la visión social y política para que el crecimiento se convierta en desarrollo. En este sentido toma importancia la última estrategia de la CEPAL de "Transformación productiva con equidad".ABSTRACT: In this paper, the main structural reforms are searched, which happened between 1970 and 1995. These are the base of the actual economic framework. The different considered periods are: the "Unidad Popular" government, the military government and the democratic transition. Finally, we must evaluate the economic results that are dependent of the implementation of the reforms and of the different development strategies. The main reforms that occurred in 1970-1973 with the "vía chilena hacia el socialismo" were: The creation of "area de propiedad social" —social property area-, the agrarian reform and the nationalisation of the most important mining companies. In the orthodox monetarism (1974-1982, the liheralisation and deregulation of the markets, the 1" Stage/Round of privatisation, the trade and the financial liberation and the seven modernizations were the most outstanding reforms, which were supported by the Chicago School. The heavy crisis which affected Latin- American countries and also Chile, caused the reforms of monetarism made possible the improvement of the effect of the reforms of the Chicago Boys. Finally the transition to democracy was am important challenge to Chilean society because Chile had the bases of a solid economy, but it was necessary to attend social and political issues to change growth into development. For this reason the last strategy of the ECLAC "Transformación productiva con equidad" is so important.

  15. Realidad de la Revista Chilena de Radiologa 2010-2014 / Chilean Journal of Radiology Facts 2010-2014

    Scientific Electronic Library Online (English)

    Marcelo, Glvez M; Patricio, Gana G; Paulina, Gana P; Sonia, Rivas H.

    Full Text Available El presente artculo tiene como finalidad la revisin editorial de la Revista Chilena de Radiologa en el perodo 2010 al 2014. Durante el perodo se publicaron 162 artculos, de los cuales 138 fueron artculos originales, artculos de revisin o presentacin de casos clnicos. Los artculos origina [...] les correspondieron a 30% de las publicaciones, los no originales un 70%. De estos ltimos, 30% fueron casos clnicos y 25%, artculos de revisin. Se rechaz el 25% de los artculos recibidos. El rea de la Radiologa con mayor representacin fue la patologa gastrointenstinal existiendo un dficit relativo de trabajos en reas relativas a tcnicas radiolgicas y radiologa intervencional. Los artculos nacionales representaron 77% y los internacionales el 23%. Dentro de los artculos nacionales, el 92% de los artculos fueron enviados de centros en Santiago y el 8% de regiones. Las citas concedidas en el perodo fueron 1875 y correspondieron principalmente a revistas norteamericanas. Las citas recibidas en el perodo fueron 138, publicadas en 60 revistas, provenientes mayoritariamente de Chile (43%) y Cuba (24%). La revista se encuentra indizada en Scielo desde el ao 2002, en la Revista Virtual del Colegio Interamericano de Radiologa CIR y en Literatura Latinoamericana y del Caribe en Ciencias de la Salud (LILACS). El factor de Impacto calculado por SciELO en el ao 2014 fue de 0,083, con 4 citas recibidas el ao 2014 de 48 artculos publicados en los aos 2012 y 2013. El artculo ms visitado en el perodo tiene ms de 220.000 visitas. La Revista Chilena de Radiologa es la sexta revista ms descargada en el periodo, con ms de 4 millones de peticiones de artculos. A travs del portal ScieELO, la revista se ha convertido en una importante ventana al trabajo cientfico de la radiologa chilena apoyado por su sociedad cientfica. Abstract in english The purpose of this article is an editorial review of the Chilean Journal of Radiology in the period 2010 to 2014, for which the publication rates, rejections, source/origin of articles and types of work and research were analyzed. During this period 162 papers were published, of which 138 correspon [...] ded to original articles (30%), revision (25%) or clinical cases (30%), while the rejection rate was 25%. Its necessary to consider that the rejection or acceptance does not always correspond to the value of the information in the manuscript, as there are many and various factors that determine the decision to accept or reject a work. It was also observed that most of the topics refer to gastrointestinal pathology, showing that there is a deficit of studies about radiological techniques and interventional radiology. As to the sources, it was found that 23% come from abroad and 77% are national articles. Within the latter, 92% are from Santiago and only 8% are from regions. There were 1,875 citations given during the period, which corresponded mainly to North American magazines. The citations received in the period were 138, published in 60 journals, mainly from Chile (43%) and Cuba (24%). The impact factor calculated by Scientific Electronic Library On Line (SciELO) in 2014 was 0,083, with four citations received during the last year from 48 articles published between 2012 and 2013. It was also found that during the period under review the most widely read magazine article, which is index-linked in SciELO since 2002, has over 220,000 viewings. At present, the Chilean Journal of Radiology is the sixth most downloaded scientific publication, exceeding 4 million requests for articles, through the SciELO electronic library, which thus makes it the best presentation card for the Chilean Radiology Society. It must be noted that this publication is also index-linked in the Revista Virtual del Colegio Interamericano de Radiologa CIR and in Literatura Latinoamericana y del Caribe en Ciencias de la Salud (LILACS).

  16. Effects of ozone exposure on 'Golden' papaya fruit by photoacoustic phase-resolved method: Physiological changes associated with carbon dioxide and ethylene emission rates during ripening

    International Nuclear Information System (INIS)

    This work addresses the effects of ozone activity on the physiology of 'Golden' papaya fruit. Depth profile analysis of double-layer biological samples was accomplished using the phase-resolved photoacoustic spectroscopy. The feasibility of the method was demonstrated by singling out the spectra of the cuticle and the pigment layers of papaya fruit. The same approach was used to monitor changes occurring on the fruit during ripening when exposed to ozone. In addition, one has performed real time studies of fluorescence parameters and the emission rates of carbon dioxide and ethylene. Finally, the amount of pigments and the changes in waxy cuticle have been monitored. Results indicate that a fruit deliberately subjected to ozone at a level of 6 ppmv underwent ripening sooner (at least 24-48 h) than a fruit stored at ambient conditions. Moreover, ozone caused a reduction in the maximum quantum yield of photosynthetic apparatus located within the skin of papaya fruit.

  17. Effects of ozone exposure on `Golden' papaya fruit by photoacoustic phase-resolved method: Physiological changes associated with carbon dioxide and ethylene emission rates during ripening

    Science.gov (United States)

    Corrêa, Savio Figueira; Mota, Leonardo; Paiva, Luisa Brito; Couto, Flávio Mota do; Silva, Marcelo Gomes da; Oliveira, Jurandi Gonçalves de; Sthel, Marcelo Silva; Vargas, Helion; Miklós, András

    2011-06-01

    This work addresses the effects of ozone activity on the physiology of `Golden' papaya fruit. Depth profile analysis of double-layer biological samples was accomplished using the phase-resolved photoacoustic spectroscopy. The feasibility of the method was demonstrated by singling out the spectra of the cuticle and the pigment layers of papaya fruit. The same approach was used to monitor changes occurring on the fruit during ripening when exposed to ozone. In addition, one has performed real time studies of fluorescence parameters and the emission rates of carbon dioxide and ethylene. Finally, the amount of pigments and the changes in waxy cuticle have been monitored. Results indicate that a fruit deliberately subjected to ozone at a level of 6 ppmv underwent ripening sooner (at least 24-48 h) than a fruit stored at ambient conditions. Moreover, ozone caused a reduction in the maximum quantum yield of photosynthetic apparatus located within the skin of papaya fruit.

  18. Formulated diets for giant Chilean frog Calyptocephalella gayi tadpoles / Dietas formuladas para renacuajos de la rana gigante chilena Calyptocephalella gayi

    Scientific Electronic Library Online (English)

    Pedro H, Toledo; Ricardo, Suazo; Maria T, Viana.

    2014-04-01

    Full Text Available Este es el primer estudio de alimentacin con dietas formuladas para evaluar la factibilidad y su efecto en el crecimiento, metamorfosis y supervivencia de renacuajos de la rana grande chilena Calyptocephalella gayi (anteriormente Caudiverbera caudiverbera) y valorar el potencial de esta especie en [...] peligro, para desarrollar la acuicultura y la repoblacin. Se realizaron tres tratamientos con dietas que contenan similares niveles de protena y energa, utilizando harina de pescado como principal fuente proteica y con sustituciones parciales con harina de vscera de ostin y Spirulina. El experimento dur 75 das, en el transcurso de los cuales los renacuajos no mostraron diferencias significativas entre los tratamientos en trminos de crecimiento y supervivencia. Este es el primer estudio que reporta efectos positivos de dietas formuladas sobre el crecimiento y supervivencia de renacuajos de la rana gigante chilena en cautiverio. Por lo tanto, se concluye que es factible cultivar, utilizando alimento formulado, renacuajos de Calyptocephalella gayi. Sin embargo, se requiere realizar ms esfuerzos y profundizar en el conocimiento de los requerimientos nutricionales para generar una dieta apropiada para esta especie en peligro. Abstract in english In this study, we conducted an experiment to evaluate the overall effect of novel diets on the growth, metamorphosis and survival of tadpoles of the endangered giant Chilean frog Calyptocephalella gayi (previously known as Caudiverbera caudiverbera) to evaluate the potential of this species for cult [...] ure and repopulation purposes. Using fishmeal as the main protein source with a substitution of either scallop viscera or Spirulina meal, three treatments were formulated to contain similar amounts of protein and energy. The experiment was run for 75 days, after which the tadpoles showed no significant differences among treatments in terms of their growth or survival. This is the first study to report positive effects of different diets on the growth and survival of tadpoles of the giant Chilean frog. Therefore, it might be possible to extend the farming of Calyptocephalella gayi for human consumption and repopulation. Nevertheless, more efforts are needed to understand their nutritional requirements to formulate appropriate diets for this endangered frog.

  19. INVENTARIO DE GASES CON EFECTO INVERNADERO EMITIDOS POR LA ACTIVIDAD AGROPECUARIA CHILENA Inventory of greenhouse gas emissions by Chilean agriculture

    Directory of Open Access Journals (Sweden)

    Rafael Novoa S.A.

    2000-04-01

    Full Text Available Se realizó una estimación de los gases con efecto invernadero emitidos por la agricultura chilena. Los resultados indican que el año 1994, la agricultura chilena emitió 321,92 Gg de metano, 21,80 Gg de N2O; 2,96 Gg de NOx y 51,97 Gg de CO. Además, se estimó que las emisiones de COVNM llegan a 2,59 Gg año-1. Estas cifras expresadas como porcentaje de las emisiones del sector no energía chileno llegan a un 74,3 % para el metano, un 5,1 % para el CO; un 93,8 % para el N2O; un 9,8 % para los NOx y un 4,9 % para los COVNM. Al sumar el potencial de calentamiento de las emisiones de metano y óxidos de N resulta que la agricultura estaría emitiendo un total equivalente a 10.504 Gg de CO2 año-1. La silvicultura, el cambio de uso del suelo y la gestión de residuos del país, generan una captación neta de CO2 de 29.709 Gg año-1, por ello se reduce este excedente en un 32 % el que quedaría en 19.205 Gg.The greenhouse gas emissions from Chilean agriculture were estimated. The results showed that during 1994, Chilean agriculture emitted 321.92 Gg of methane; 21.80 Gg of N2O; 2.96 Gg of NOx and 51.97 Gg of CO. Also, agriculture generated 2.59 Gg year-1 of non-methane volatile compound emissions (NMVOC. These figures as a percentage of the non-energy sector emissions are as follows: 74.3% for methane; 5.1 % for CO; 93.8 % for N2O; 9.8 % for NOx and 4.9 % for NMVOC. Taking into account the potential warming effects of methane and nitrous oxide as CO2 equivalent amounts, agriculture is responsible for 10,504 Gg CO2 year-1. Since forestry, land-use changes and handling of residues in Chile represent a net capture of 29,709 CO2 Gg year-1, agriculture reduces this surplus to 35.4 %. So, the total surplus is about 19.205 Gg year-1.

  20. Posfolklore: Raíces y globalización en la música popular chilena

    Directory of Open Access Journals (Sweden)

    González, Juan Pablo

    2011-10-01

    Full Text Available This article discusses the development of the concept of folk roots, introduced by Chilean singer-songwriters in the mid-seventies, as a strategy to legitimize their divergent practice from the norm of folklore in times of military rule. The institutionalization of this concept with the return of democracy in the early nineties and its continued tightening from a third generation of Chilean singersongwriters and bands derivatives from theater companies, allow us to discuss the problem of folk roots in times of globalization. We propose the concepts of posfolklore and hydroponic roots, attached to the trend of Chilean listeners and musicians to incorporate world music to their artistic practices and consumption. These concepts would facilitate the discussion of the mixing of influences in Chilean music and the consequent proliferation of intermediate rather than hegemonic identities in contemporary Chilean culture.

    Este artículo aborda el desarrollo del concepto de raíz folklórica, introducido por cantautores chilenos a mediados de los años setenta, como estrategia para legitimar corrientes divergentes del folklore de la norma imperante en tiempos del régimen militar. La institucionalización de este concepto con el retorno a la democracia y su continuo tensionamiento desde una tercera generación de cantautores nacionales y desde los nuevos grupos musicales derivados de compañías teatrales, permite reflexionar sobre el problema de las raíces en tiempos de globalización. Se proponen los conceptos de posfolklore y de raíces hidropónicas, adscritos a la permanente tendencia del músico y del auditor chileno a incorporar músicas del mundo a su práctica y consumo musical. Estos conceptos permiten discutir las mezclas en la música chilena y la consiguiente multiplicación de identidades intermedias más que hegemónicas en la cultura chilena contemporánea.

  1. Cantidad, viabilidad y germinabilidad de los granos de polen de Carica papaya L. Quantity, viability and germinability of pollen grains in Carica papaya L.

    Directory of Open Access Journals (Sweden)

    J Parés-Martínez

    2006-06-01

    Full Text Available A fin de contribuir al conocimiento de la biología floral de la lechosa cv. Cartagena Amarilla, se determinó la cantidad, viabilidad y germinabilidad de los granos de polen en flores masculinas y hermafroditas, según la clasificación de Storey. Tanto la cantidad como la viabilidad fueron determinadas mediante la metodología del hematocitómetro, utilizando como colorante azul de anilina en lactofenol al 1%. Para la germinabilidad se usó una solución de sacarosa al 5%. Los resultados indicaron marcadas diferencias entre los tipos florales. Así, los tipos florales IV, IV+ y V produjeron la mayor cantidad de granos de polen por flor, comparados con los tipos II y III. Los porcentajes de viabilidad fueron 6, 22, 69, 73 y 91% para los tipos florales II, III, IV, IV+ y V, respectivamente. Mientras que, los porcentajes de germinabilidad fueron de 11, 19, 71, 77 y 92% para los tipos II, III, IV, IV+ y V, respectivamente, Estos resultados permiten concluir que a medida en que los tipos florales tienden hacia la masculinidad incrementa la cantidad, viabilidad y germinabilidad de sus granos de polen.With the aim of contributing to the knowledge of the floral biology of papaya cv. Cartagena Amarilla, quantity, viability and germinability were determined in grains of pollen in masculine and hermaphrodite flowers, according to the classification of Storey. Quantity as well as viability was determined using a hemacytometer, using aniline blue in lactophenol at 1%. For the germinability a sucrose solution was used at 5%. The results indicated market differences among floral types. Thus, floral types IV, IV+ and V produced the highest quantity of pollen grains per flower, compare to types II and III. Viability percentages were 6, 22, 69, 73 and 91% for floral types II, III, IV, IV+ and V respectively. While, germinability percentages were 11, 19, 71, 77 and 92% for types II, III, IV, IV+ and V, respectively. These results allow to conclude that at the time that floral types tend to masculinity increase the quantity, viability and germinability of its grains of pollen.

  2. Novel concepts on functional foods and nutrigenomics in healthy aging and chronic diseases: a review of fermented papaya preparation research progress

    Directory of Open Access Journals (Sweden)

    Francesco Marotta

    2012-05-01

    Full Text Available AbstractFunctional foods are an emerging research field corresponding with genomical, epidemiological and clinical studies integrated with the food industry in accordance with the consumer demands. Consequently, the features of the functional foods are being discussed by various researchers and related institutions, and a common view has been pointed out about the availability and the nature of the components of functional foods. Recently, the outcomes of functional foods are being assessed by the help of all the available scientific tools. Genomic medicine is one of the most promising areas of research to reveal the benefits of functional foods and the bioactive ingredients. Nutrigenomics aims at studying the genetic and epigenetic interactions with a nutrient or the functional component in order to lead to a phenotype change and therefore to the cell metabolism, differentiation or even apoptosis. Papaya and its fermentation product arespecific products derived from the technologically advanced and controlled environmentalfriendly bio-fermentation process. Functional Foods in Health and Disease 2012, 2(5:120-136 It has been well known for a long time that the natural anti-oxidant properties of papaya, mainly depending on vitamins A and C in addition to certain amino acids, were consistent both in the fruit and derived from the papain enzyme which is no longer present in the fermented product. In this article, functional foods in genomic medicine are discussed in review of the fermented papaya preparation research progress. Clinical evidences about fermented papaya as a functional food are reported as supported by various research protocols and experimental models. The benefits of fermented papaya preparation are also discussed in nutrigenomic basis and it is reported to have an important antioxidant and transcriptomic potential which deserves further investigation. As a conclusion, fermented papaya preparation represents a Functional Food highly compliant with the novel features of the new nutrigenomic-driven action plan strategy aimed to reduce the incidences of diseases and successful integration within specific pharmacological treatments.

  3. Anlise de sistemas gene marcador/ agente seletivo alternativos para seleo positiva de embries somticos transgnicos de mamoeiro / Analysis of marker gene/ selective agent systems alternatives to positive selection of transgenic papaya (Carica papaya L.) Somatic embryos

    Scientific Electronic Library Online (English)

    M. T., SOUZA JNIOR; M. F., VENTUROLI; M. C. F., COELHO; E. L., RECH FILHO.

    Full Text Available Questes relacionadas biossegurana de organismos geneticamente modificados e percepo pblica destes tm levado as instituies envolvidas no desenvolvimento comercial de plantas transgnicas a abandonarem o uso de genes marcadores que conferem resistncia a antibiticos. O desenvolvimento de [...] mamoeiros (Carica papaya L.) transgnicos tem sido baseado, at o momento, no uso de um nico gene marcador, o gene npt II, que confere resistncia a antibiticos como canamicina e neomicina. O presente estudo objetivou avaliar os sistemas alternativos manA/ manose e bar/ PPT como sistemas gene marcador/ agente seletivo para a variedade Sunrise de mamoeiro. O desenvolvimento de embries somticos secundrios a partir de embries primrios foi avaliado em meio de induo de embriognese suplementado com manose e/ou sacarose. Concentraes variando entre 0,1 e 120 g/l de manose foram testadas como nica fonte de carbono, ou em combinao com sacarose. O desenvolvimento de embries somticos secundrios a partir dos primrios em meio suplementado com at 120 g.L-1 de manose, como nica fonte de carbono, demonstrou que este sistema no passvel de uso no desenvolvimento de mamoeiros transgnicos. Quanto ao sistema bar/ PPT, avaliou-se o desenvolvimento de embries somticos primrios e secundrios em meio suplementado com concentraes de PPT variando de zero a 150 miM. No foi observado desenvolvimento de embries em meio suplementado com concentrao igual ou superior a 125 miM, o que sugere o uso desta concentrao para a seleo de embries transgnicos de mamoeiro. Abstract in english Questions relative to biosafety and public perception of genetically modified organisms have taken those involved on the development of transgenic plants to a phase out on the use of antibiotic resistance marker genes. The development of transgenic papayas (Carica papaya L.) has been based so far on [...] the use of one only marker gene, the npt II gene, which confers resistance to antibiotics such as kanamycin and neomycin. The present study aimed to evaluate the systems manA/ mannose and bar/ PPT as alternatives marker gene/ selective agent systems to be used on the development of transgenic Sunrise papayas. Therefore, the development of secondary somatic embryos from primary ones was evaluated on embryogenesis induction medium supplemented with mannose and/or sucrose. Mannose concentration ranging from 0.1 to 120 g.L-1 were tested as sole carbon source, or in conjunction with sucrose. The development of secondary somatic embryos on medium supplement with up to 120 g/l, as sole carbon source, demonstrated that this system is not applicable for the development of transgenic papayas. When evaluating the bar/ PPT system, the development of primary and secondary somatic embryos was checked for on medium supplement with zero to 150 muM of PPT. No somatic embryos developed on medium supplemented with PPT at 125 muM or more, what suggests the use of this concentration for the in vitro selection of transgenic papaya embryos.

  4. MONITORING OF ORGANOPHOSPHOROUS PESTICIDE RESDUES IN SAMPLES OF BANANA, PAPAYA, AND BELL PEPPER

    Scientific Electronic Library Online (English)

    Mariana F., Lemos; Mayara F., Lemos; Henrique P., Pacheco; Rodrigo, Scherer.

    2015-02-01

    Full Text Available The objective of this study was to monitor 11 organophosphorus pesticides in samples of papaya, bell pepper, and banana, commercialized in the metropolitan area of Vitria (ES, Brazil). The pesticides were determined by an optimized and validated method using high performance liquid chromatography w [...] ith tandem mass spectrometry (HPLC-MS/MS). All three samples exhibited a matrix effect for most of the pesticides, mainly with signal suppression, and therefore the calibration curves were produced in matrices. Linearity revealed coefficients of determination (r2) greater than 0.9895 for all pesticides and recovery results ranged from between 76% and 118% with standard deviation no greater than 16%. Precision showed relative standard deviation values lower than 19% and HorRat values lower than 0.7, considering all pesticides. Limits of quantification were less than 0.01 mg/kg for all pesticides. Regarding analysis of the samples (50 of each), none of the pesticides exceeded the maximum residue limit determined by Brazilian legislation.

  5. Herbivorous mites as ecological engineers: indirect effects on arthropods inhabiting papaya foliage.

    Science.gov (United States)

    Fournier, Valrie; Rosenheim, Jay A; Brodeur, Jacques; Laney, Lee O; Johnson, Marshall W

    2003-05-01

    We examined the potential of a leaf roller to indirectly influence a community of arthropods. Two mite species are the key herbivores on papaya leaves in Hawaii: a spider mite, Tetranychus cinnabarinus Boisduval, and an eriophyid mite, Calacarus flagelliseta, which induces upward curling of the leaf margin at the end of the summer when populations reach high densities. A survey and three manipulative field experiments demonstrated that (1) leaf rolls induce a consistent shift in the spatial distribution of spider mites and their predators, the coccinellid Stethorus siphonulus Kapur, the predatory mites Phytoseiulus spp., and the tangle-web building spider Nesticodes rufipes Lucas; (2) the overall abundance of spiders increases on leaves with rolls; (3) the specialist predators Stethorus and Phytoseiulus inhabit the rolls in response to their spider mite prey; and (4) the spider inhabits the rolls in response to the architecture of the roll itself. This study shows the importance of indirect effects in structuring a terrestrial community of herbivores. PMID:12721835

  6. Radiations and biodegradation techniques for detoxifying Carica papaya seed oil for effective dietary and industrial use.

    Science.gov (United States)

    Afolabi, Israel Sunmola; Bisi-Adeniyi, Tolulope Dorcas; Adedoyin, Toluwalase Ronke; Rotimi, Solomon Oladapo

    2015-10-01

    Benzyl isothiocyanate (BITC) is toxic in high concentration. The capacity of Aspergillus niger, microwave and ultraviolet radiations to reduce the BITC levels in Carica papaya Linn seed oil were assessed in vitro. BITC at different concentrations were periodically exposed to microwave and ultraviolet radiations for 30 min and 10 h, respectively; and to identify Aspergillus niger for 4 days. Microwave radiation significantly reduced (p < 0.05) BITC levels (0.0272, 0.0544, and 0.0816 μmol) to 12.19, 8.99 and 27.5 % respectively within 15 min. Ultraviolet radiation significantly reduced (p < 0.05) BITC levels at all the concentrations. A. niger significantly increased (p < 0.05) BITC degradation on days 2 and 4 at 0.816, 1.36 and 2.72 nmol. Glutathione activity was significantly increased (p < 0.05) while glutathione S-transferase activity significantly reduced (p < 0.05) at all concentrations on days 3 and 4 respectively. The three techniques are possible models for improving the dietary consumption of the oil. PMID:26396392

  7. Immobilization of papaya laccase in chitosan led to improved multipronged stability and dye discoloration.

    Science.gov (United States)

    Jaiswal, Nivedita; Pandey, Veda P; Dwivedi, Upendra N

    2016-05-01

    A purified papaya laccase was immobilized in chitosan beads using entrapment approach and its physico-chemical properties were investigated and compared with that of free enzyme. Increase in properties of the laccase such as optimum temperature (by 10°C), thermostability (by 3-folds) and optimum pH (from 8.0 to 10.0) was observed after immobilization. Immobilization led to increased tolerance of enzyme to a number of metal ions (including heavy metals) and organic solvents namely, ethanol, isopropanol, methanol, benzene and DMF. The catalytic efficiency (Kcat/Km) of the immobilized enzyme was found to increase more than ten folds, in comparison to that of the free enzyme, with hydroquinone as substrate. Immobilization of laccase also led to improvement in dye decolorization such that the synthetic dye indigo carmine (50μg/ml) was completely decolorized within 8h of incubation as compared to that of the free laccase which decolorized the same dye to only 56% under similar conditions. Thus, immobilization of laccase into chitosan beads led to tremendous improvement in various useful attributes of this enzyme thereby making it more versatile for its industrial exploitation. PMID:26812115

  8. Characterization and occurence of eairly softening disorder in 'golden' papaya fruits

    Directory of Open Access Journals (Sweden)

    Angelo Pedro Jacomino

    2010-12-01

    Full Text Available The occurrence of green skin and soft pulp in 'Golden' papaya fruit during certain seasons has been reported by farmers in the northern of the state of Espirito Santo, Brazil. The objective of this study was to characterize and determine the occurrence of this disorder, which was referred as "early softening disorder". Fruits were harvested weekly for 11 months (from September to July. The fruits were stored at 10°C, and then fruit flesh firmness and skin color were analyzed. The results of the firmness test were submitted to regression analysis assuming a linear trendline. The slope of the curve was called the 'softening index' (SI. Fruits with early softening are characterized by a loss of firmness in less than 10 days, even when stored under refrigeration. Although softened, the skin of the fruit remains partially green. Fruits with the disorder occurred more frequently from mid-summer to mid-autumn (February to May. It is not possible to distinguish early softening disorder fruits from those without the disorder by skin color and flesh firmness analysis at the time of the harvest.

  9. Protective Action of Carica papaya on β-Cells in Streptozotocin-Induced Diabetic Rats.

    Science.gov (United States)

    Miranda-Osorio, Pedro H; Castell-Rodríguez, Andrés E; Vargas-Mancilla, Juan; Tovilla-Zárate, Carlos A; Ble-Castillo, Jorge L; Aguilar-Domínguez, Dora E; Juárez-Rojop, Isela E; Díaz-Zagoya, Juan C

    2016-01-01

    The aim of the present study was to investigate the effect of C. papaya L. leaf extract (CPLE) on pancreatic islets in streptozotocin (STZ)-induced diabetic rats, as well as on cultured normal pancreatic cells with STZ in the medium. CPLE (3-125 mg/Kg) was administered orally for 20 days, while a group of diabetic rats received 5 IU/Kg/day of insulin. At the end of the treatment the rats were sacrificed. Blood was obtained to assess glucose and insulin levels. The pancreas was dissected to evaluate β cells by immunohistochemistry. In addition, normal pancreatic cells were cultured in a medium that included CPLE (3-12 mg). One half of the cultured cells received simultaneously CPLE and STZ (6 mg), while the other half received CPLE and five days later the STZ. After three days of incubation, insulin was assayed in the incubation medium. The CPLE administered to diabetic rats improved the fasting glycemia and preserved the number and structure of pancreatic islets. However, when CPLE was added to pancreatic cells in culture along with STZ, the insulin concentration was higher in comparison with the cells that only received STZ. In conclusion, the CPLE preserves the integrity of pancreatic islets, improves the basal insulin secretion and protects cultured cells from the adverse effects of STZ. PMID:27128930

  10. Imobilisasi Crude Enzim Papain Yang Diisolasi Dari Getah Buah Pepaya (Carica papaya L) Dengan Menggunakan Kappa Karagenan Dan Kitosan Serta Pengujian Aktivitas Dan Stabilitasnya

    OpenAIRE

    Wibisono, Eko

    2011-01-01

    Crude papain enzyme has been isolated from papaya fruit latex (Carica papaya L) with Balls and Lineweaver method, where the crude papain enzyme was immobilized by entrapping the lattice type by using the kappa carrageenan and chitosan, and then tested its activity with the Murachi method. The activity of free crude papain enzyme 82.493 μg/ml at a temperature of 55oC and pH 7, the immobilized crude enzyme papain with kappa carrageenan 78.706 μg/ml at a temperature of 60oC and pH 6.5, and the i...

  11. Efecto de la biofertilización y los biorreguladores en la germinación y el crecimiento de Carica papaya L.

    OpenAIRE

    Maricela Constantino; Regino Gómez-Álvarez; David Álvarez-Solís; Juan Manuel Pat-Fernández; Elda Guadalupe Espín

    2011-01-01

    Título en inglés: Effect of biofertilization and bioregulators on germination and growth of Carica papaya L. Resumen Con el objetivo de incrementar y acelerar el proceso de germinación de las semillas y obtener una alta producción y homogeneidad de plántulas de Carica papaya variedad Maradol en vivero, se evaluó el efecto de tres biofertilizantes aplicados solos o en combinación (Azotobacter chroococcum, Azospirillum brasilense y Glomus intraradices), y un biorregulador del crecimient...

  12. Efecto de la biofertilización y los biorreguladores en la germinación y el crecimiento de carica papaya l.

    OpenAIRE

    Constantino, Maricela; Gómez-Álvarez, Regino; Álvarez-Solís, David; Pat-Fernández, Juan Manuel; Espín, Elda Guadalupe

    2011-01-01

    Título en inglés: Effect of biofertilization and bioregulators on germination and growth of Carica papaya L. Resumen Con el objetivo de incrementar y acelerar el proceso de germinación de las semillas y obtener una alta producción y homogeneidad de plántulas de Carica papaya variedad Maradol en vivero, se evaluó el efecto de tres biofertilizantes aplicados solos o en combinación (Azotobacter chroococcum, Azospirillum brasilense y Glomus intraradices), y un biorregulador del crecimient...

  13. Photoacoustic study of the influence of the cooling temperature on the CO2 emission rate by Carica papaya L. in modified atmosphere

    Science.gov (United States)

    Schramm, D. U.; Sthel, M. S.; da Silva, M. G.; Carneiro, L. O.; Silva, H. R. F.; Martins, M. L. L.; Resende, E. D.; Vitorazi, L.; Vargas, H.

    2005-06-01

    The monitoring of trace gas emitted by papaya fruits and assessments of its mass loss can contribute to improve the conditions for their storage and transport. The C02 emission rate by the papaya fruits, monitored by a commercial infrared-based gas analyzer, was influenced by the temperature and storage time. The fruits stored at temperature of 13 °C accumulated more CO2 inside the PEBD bags than those fruits stored at 6 °C. The loss of mass of the fruits progressively increased with storage time for both temperatures until the saturation of the moisture inside the PEBD bag, been more pronounced at 13 ºC.

  14. EXTRAÍBLES DE CORTEZAS CHILENAS: EFECTO SOBRE LA LUMINISCENCIA DE BACTERIAS EFFECT OF CHILEAN BARK EXTRACTS ON BACTERIAL LUMINESCENCE

    Directory of Open Access Journals (Sweden)

    Hernán Poblete

    2007-01-01

    Full Text Available Se determinó la disminución de la bioluminiscencia de bacterias como indicador de la actividad biológica de extractos de corteza. Se analizaron cuatro cortezas nativas chilenas. La reducción de la bioluminiscencia fue diferente dependiendo de la especie de corteza. El aumento del tiempo de un tratamiento térmico de la corteza (24, 48, 72 y 144 horas con 103ºC produjo una reducción de la actividad biológica de los extractos. El estudio indica que los terpenos podrían ser responsables del efecto señaladoBioluminescence decrease of bacteria as a biological activity indicator for bark water extracts was determinate. Four Chilean barks were tested. A different reduction on bioluminescence depending on bark species was observed. Increasing time of thermal treatment of bark (24, 48, 72 and 144 hours with 103ºC produced a reduction of the biological activity of extracts. The study indicates that the terpenes could be responsible for the described effect

  15. Estudiantes con discapacidad en una universidad chilena: desafíos de la inclusión

    Directory of Open Access Journals (Sweden)

    Gabriela Paulina VILLAFAÑE HORMAZÁBAL

    2016-01-01

    Full Text Available El propósito de este artículo es dar cuenta de una investigación acerca de las condiciones de inclusión para los estudiantes con discapacidad en una universidad chilena. La investigación tiene un diseño cuantitativo, descriptivo y transversal. Para la recolección de datos se elaboró una encuesta que fue aplicada a 38 estudiantes con discapacidad. Los principales resultados revelan un alto nivel de retención de los estudiantes, quienes presentan una percepción positiva de su inclusión en la vida universitaria, así como una alta satisfacción con la mayoría de los servicios. Se destaca que siete de cada diez encuestados reconocen haber recibido algún tipo de atención educativa para realizar sus estudios. Sin embargo, existe una falta de articulación entre las iniciativas de la Universidad para favorecer el acceso y permanencia de personas con discapacidad; a esto se agrega la carencia de protocolos y de instancias de capacitación para profesores y administrativos. Se propone que la institución establezca un sistema de gestión que defina objetivos, estrategias y acciones para contribuir a la inclusión de personas con discapacidad.

  16. CUL ES EL CRECIMIENTO DE LARGO PLAZO DE LA ECONOMA CHILENA? UNA RESPUESTA FORMAL PARA UNA ANTIGUA PREGUNTA

    Scientific Electronic Library Online (English)

    Byron, Idrovo.

    2010-12-30

    Full Text Available El objetivo del presente estudio es estimar la tasa de crecimiento de la economa en un contexto de plena utilizacin de los recursos productivos. Se emplean para el anlisis modelos univariados de series de tiempo, incluyendo una tendencia determinstica (con quiebres de medias), y un modelo de est [...] ado-espacio que considera una tendencia estocstica y un componente cclico, estimado por Filtro de Kalman. El componente cclico es calculado con y sin cambios de rgimen (Markov-switching models), de acuerdo con la idea de Friedman (1993) -plucking model. Los resultados indican que la tasa de crecimiento potencial de la economa chilena sera superior al 4% anual, acompaada de ciclos econmicos asimtricos. Abstract in english The objective of the present study is to estimate the economy growth rate in the context of full use of the productive resources. Time series univariete models, including a deterministic tendency (with mean breaks), and a space-state model that considers a stochastic tendency and a cyclic component, [...] estimated by Kalmans filter were used. The cyclic component is calculated with and without regime chages (Markov-switching models), according to Friedmans idea -plucking model. The results indicate that the Chilean economy potential growth rate would be greater than 4% annually, accompanied by economic asymmetric cycles.

  17. Integración metodológica para el estudio del texto de las sentencias penales chilenas

    Directory of Open Access Journals (Sweden)

    Claudio Antonio Agüero San Juan

    2010-01-01

    Full Text Available Proponemos una metodología para describir la estructura y el funcionamiento del texto "sentencia penal chilena" y evaluar la calidad de su argumentación. La importancia de estos procesos radica en que la sentencia es un caso paradigmático del modo en que las sociedades construyen lo bueno y lo malo. En virtud de la especificidad del género y del registro lingüístico del texto en estudio, integramos elementos de pragma-dialéctica, de lingüística sistémico funcional y de Modified Wigmorean Analysis para suplir las desventajas individuales de cada metodología y optimizar sus ventajas. Se revisa someramente el aparato crítico que sustenta cada una de las estrategias de análisis, luego, a través del estudio de un fragmento de una sentencia real, demostramos la factibilidad de la integración y concluimos revisando la posibilidad de usar esta propuesta en comparaciones intertextuales e interdiscursivas.

  18. Bionomics of the apefly, Spalgis epius (Lepidoptera: Lycaenidae, predatory on the papaya mealybug, Paracoccus marginatus (Hemiptera: Pseudococcidae, in Thailand

    Directory of Open Access Journals (Sweden)

    Intawat Burikam

    2012-02-01

    Full Text Available We investigated the life history, growth ratio and feeding potential of the hemipterophagous butterfly or the apefly,Spalgis epius (Westwood (Lepidoptera: Lycaenidae: Miletinae, a promising augmentative biological control agent of thepapaya mealybug, Paracoccus marginatus Williams & Granara de Willink (Hemiptera: Pseudococcidae, which is an invasivealien species detected in 2008 in Thailand. The investigation was carried out under laboratory conditions at 25-30C and 40-60% RH using papaya mealybug reared on Thai pumpkin as its prey. The mean duration of the egg, larval and pupal stageswere 3.540.50, 12.012.40, and 10.320.88 days, respectively, and the mean duration from egg to adult emergence was 25.873.78 days. The geometric growth ratio of successive larval instars using the head capsule width as a parameter was 1.65 andconformed to Dyars Law. As for the predatory potential of S. epius, the total number of papaya mealybug consumed duringthe larval stage were 4,115.75553.28 eggs, 281.2545.08 nymphs and 77.5016.52 female adults. The instar-specific preyconsumption from the first to fifth larval instars in the order of eggs, nymphs and female adults were 170.0026.32, 4.251.71and 2.000.82; 654.0067.97, 35.3512.48 and 10.254.29; 1,376.75130.95, 93.2511.70 and 23.503.94; 1,426.50252.93,110.7515.60 and 31.005.25; and 31.005.25, 37.753.59 and 10.752.22, respectively. These findings warrant furtherinvestigation into the amenability and suitability of the entomophagous apefly, S. epius, for as an integrated pest managementstrategy for the augmentative biological control of the papaya mealybug in Thailand.

  19. Chronic Hepatotoxicity and Nephrotoxicity Study of Orally Administered Aqueous and Ethanolic Extracts of Carica papaya Seeds in Adult Wistar Rats

    Directory of Open Access Journals (Sweden)

    Umana Uduak

    2013-08-01

    Full Text Available About 80% of the population in many third world countries still use traditional medicine (medicinal plants as the sole means of therapy against diseases. Carica papaya s a medicinal plant which has been proven to contain substances that can be used for such purposes. Some of its uses include anti-ulcerogenic, anti-fungal, anti-microbial, anti-tumour and it is employed in wound-healing activity and antifertility activity to mention a few. This study was designed to evaluate the chronic hepatotoxic and nephrotoxic effects of orally administered aqueous and ethanolic extracts of Carica papaya seed in adult Wistar rat. The up-and-down method as outlined in the OECD guidelines was used to determine the LD50. Twenty five adult Wistar rats weighing between 160-220 gm were used for the study. They were randomised and five assigned to each of the treatment groups. Group one was the control, group two and three received 100 mg/kg and 1000 mg/kg body weight of aqueous extract respectively while groups four and five received 100 mg/kg and 1000 mg/kg body weight of ethanolic extract respectively. They were all dosed orally for 60 days. The results of the haematological indices, renal function test and liver function test for both extracts were normal and so also were the organ body weight ratios. The histological studies of the liver, kidneys and spleen did not reveal any pathologic changes when compared to the control group. In Conclusion it can be said that aqueous and ethanolic extracts of Carica papaya seed extract is not hepatotoxic and nephrotoxic to adult Wistar rats.

  20. Biological control of toxigenic citrus and papaya-rotting fungi by Streptomyces violascens MT7 and its extracellular metabolites.

    Science.gov (United States)

    Choudhary, Bharti; Nagpure, Anand; Gupta, Rajinder K

    2015-12-01

    An Indian indigenous, Loktak Lake soil isolate Streptomyces violascens MT7 was assessed for its biocontrol potential both in vitro and in vivo against toxigenic fruit-rotting fungi. Strain MT7 exhibited broad-spectrum antifungal activity against various pathogenic postharvest fungi of citrus and papaya. In shake-flask fermentation, antagonist S. violascens MT7 highly produced extracellular antifungal metabolites in early stationary growth phase in glucose-yeast extract-malt extract (M93) broth. Both extracellular culture fluid (ECF) and its n-butanol extract showed significant broad-spectrum fungal mycelial inhibition of several tested fruit-rotting fungi. Antifungal metabolite was found to be heat stable, nonpeptidic, and polyene type antibiotic. The lowest minimum inhibitory concentration (MIC) of n-butanol extract against Colletotrichum gloeosporioides MTCC 9664 and Aspergillus niger MTCC 281 was 0.0312 and 0.0625 mg/ml, respectively. Purification of n-butanol extract through silica gel chromatography resulted in partial purification of bioactive metabolite and the TLC autobiography revealed the presence of single antifungal metabolite with Rf value of 0.755. In vivo bioassays demonstrated the biocontrol potential of tested biocontrol agents on fruit-rotting fungi. Use of cell suspension of S. violascens MT7, extracellular metabolite(s), and n-butanol extract significantly (p papaya fruits. Therefore, these results strongly suggest a high potential for application of S. violascens MT7 and its extracellular metabolites as an effective eco-friendly alternative to synthetic fungicides for controlling toxigenic citrus and papaya-rotting fungi. PMID:26214840

  1. Flavonoid from Carica papaya inhibits NS2B-NS3 protease and prevents Dengue 2 viral assembly

    OpenAIRE

    Senthilvel, Padmanaban; Lavanya, Pandian; Kumar, Kalavathi Murugan; Swetha, Rayapadi; Anitha, Parimelzaghan; Bag, Susmita; Sarveswari, Sundaramoorthy; Vijayakumar, Vijayaparthasarathi; Ramaiah, Sudha; Anbarasu, Anand

    2013-01-01

    Dengue virus belongs to the virus family Flaviviridae. Dengue hemorrhagic disease caused by dengue virus is a public health problem worldwide. The viral non structural 2B and 3 (NS2B-NS3) protease complex is crucial for virus replication and hence, it is considered to be a good anti-viral target. Leaf extracts from Carica papaya is generally prescribed for patients with dengue fever, but there are no scientific evidences for its anti-dengue activity; hence we intended to investigate the anti-...

  2. Acarofauna associated to papaya orchards in Veracruz, Mexico / Acarofauna asociada a huertas de papayo en Veracruz, Mxico

    Scientific Electronic Library Online (English)

    Marycruz, Abato-Zrate; Juan A., Villanueva-Jimnez; Gabriel, Otero-Colina; Catarino, vila-Resndiz; Elas, Hernndez-Castro; Noel, Reyes-Prez.

    Full Text Available Productores agrcolas en Mxico recientemente notaron un fuerte incremento en las infestaciones de caros en las huertas de papayo (Carica papaya L. 1753). Se elabor una lista de las especies de caros asociados con hojas de papayo para determinar las especies responsables de las altas infestacione [...] s y para identificar a los caros depredadores. Los caros fueron colectados de tres estratos (alto, medio y bajo) en siete muncipios del centro del estado de Veracruz. Las hojas fueron procesadas por lavado y tamizado. Las especies identificadas incluyeron cuatro tetranquidos: Eotetranychus lewisi (McGregor 1943), Eutetranychus banksi (McGregor 1914), Tetranychus merganser Boudreaux 1954 y Tetranychus urticae Koch 1836; tres fitoseidos: Euseius hibisci (Chant 1959), Galendromus helveolus (Chant 1959) y Phytoseiulus macropilis (Banks 1904), donde las dos primeras especies fueron las ms abundantes. El caro erifido errante Calacarus citrifolii Keifer 1955 fue colectado en tres municipios, en el estrato bajo. El caro blanco, Polyphagotarsonemus latus (Banks 1904), y el caro carmn, Tetranychus cinnabarinus (Boisduval 1867), no fueron colectados, aunque estas dos especies fueron registradas previamente en esta rea. Ninguno de los fitoseidos encontrados puede ser considerado de reciente establecimiento en el rea; se discute su potencial como agentes de control biolgico. Abstract in english Mexican agriculturists have recently noticed strong increases of mite infestations in papaya (Carica papaya L. 1753) orchards. A list of mite species associated with papaya leaves was constructed to determine the species responsible for high infestations and to identify predaceous mites as potential [...] biological control agents. Mites were collected from three foliage strata (high, middle and low), in seven municipalities of central Veracruz State. Leaves were processed by washing and sieving. Identified species included four tetranychids: Eotetranychus lewisi (McGregor 1943), Eutetranychus banksi (McGregor 1914), Tetranychus merganser Boudreaux 1954, and Tetranychus urticae Koch 1836; as well as three phytoseiids: Euseius hibisci (Chant 1959), Galendromus helveolus (Chant 1959) and Phytoseiulus macropilis (Banks 1905), with the first two species being the most abundant. The vagrant eriophyid Calacarus citrifolii Keifer 1955 was collected in three municipalities, in the low stratum. Neither the broad mite, Polyphagotarsonemus latus (Banks 1904), nor the carmine spider mite, Tetranychus cinnabarinus (Boisduval 1867), were collected, although these species were previously recorded from this area. None of the Phytoseiid species found can be considered a recently established species; their potential as biological control agents is discussed.

  3. Traditional Aboriginal Preparation Alters the Chemical Profile of Carica papaya Leaves and Impacts on Cytotoxicity towards Human Squamous Cell Carcinoma

    Science.gov (United States)

    Nguyen, Thao T.; Parat, Marie-Odile; Shaw, Paul N.; Hewavitharana, Amitha K.; Hodson, Mark P.

    2016-01-01

    Carica papaya leaf decoction, an Australian Aboriginal remedy, has been used widely for its healing capabilities against cancer, with numerous anecdotal reports. In this study we investigated its in vitro cytotoxicity on human squamous cell carcinoma cells followed by metabolomic profiling of Carica papaya leaf decoction and leaf juice/brewed leaf juice to determine the effects imparted by the long heating process typical of the Aboriginal remedy preparation. MTT assay results showed that in comparison with the decoction, the leaf juice not only exhibited a stronger cytotoxic effect on SCC25 cancer cells, but also produced a significant cancer-selective effect as shown by tests on non-cancerous human keratinocyte HaCaT cells. Furthermore, evidence from testing brewed leaf juice on these two cell lines suggested that the brewing process markedly reduced the selective effect of Carica papaya leaf on SCC25 cancer cells. To tentatively identify the compounds that contribute to the distinct selective anticancer activity of leaf juice, an untargeted metabolomic approach employing Ultra High Performance Liquid Chromatography-Quadrupole Time of Flight-Mass Spectrometry followed by multivariate data analysis was applied. Some 90 and 104 peaks in positive and negative mode respectively were selected as discriminatory features from the chemical profile of leaf juice and >1500 putative compound IDs were obtained via database searching. Direct comparison of chromatographic and tandem mass spectral data to available reference compounds confirmed one feature as a match with its proposed authentic standard, namely pheophorbide A. However, despite pheophorbide A exhibiting cytotoxic activity on SCC25 cancer cells, it did not prove to be the compound contributing principally to the selective activity of leaf juice. With promising results suggesting stronger and more selective anticancer effects when compared to the Aboriginal remedy, Carica papaya leaf juice warrants further study to explore its activity on other cancer cell lines, as well as investigation to confirm the identity of compounds contributing to its selective effect, particularly those compounds altered by the long heating process applied during the traditional Aboriginal remedy preparation. PMID:26829042

  4. Traditional Aboriginal Preparation Alters the Chemical Profile of Carica papaya Leaves and Impacts on Cytotoxicity towards Human Squamous Cell Carcinoma.

    Science.gov (United States)

    Nguyen, Thao T; Parat, Marie-Odile; Shaw, Paul N; Hewavitharana, Amitha K; Hodson, Mark P

    2016-01-01

    Carica papaya leaf decoction, an Australian Aboriginal remedy, has been used widely for its healing capabilities against cancer, with numerous anecdotal reports. In this study we investigated its in vitro cytotoxicity on human squamous cell carcinoma cells followed by metabolomic profiling of Carica papaya leaf decoction and leaf juice/brewed leaf juice to determine the effects imparted by the long heating process typical of the Aboriginal remedy preparation. MTT assay results showed that in comparison with the decoction, the leaf juice not only exhibited a stronger cytotoxic effect on SCC25 cancer cells, but also produced a significant cancer-selective effect as shown by tests on non-cancerous human keratinocyte HaCaT cells. Furthermore, evidence from testing brewed leaf juice on these two cell lines suggested that the brewing process markedly reduced the selective effect of Carica papaya leaf on SCC25 cancer cells. To tentatively identify the compounds that contribute to the distinct selective anticancer activity of leaf juice, an untargeted metabolomic approach employing Ultra High Performance Liquid Chromatography-Quadrupole Time of Flight-Mass Spectrometry followed by multivariate data analysis was applied. Some 90 and 104 peaks in positive and negative mode respectively were selected as discriminatory features from the chemical profile of leaf juice and >1500 putative compound IDs were obtained via database searching. Direct comparison of chromatographic and tandem mass spectral data to available reference compounds confirmed one feature as a match with its proposed authentic standard, namely pheophorbide A. However, despite pheophorbide A exhibiting cytotoxic activity on SCC25 cancer cells, it did not prove to be the compound contributing principally to the selective activity of leaf juice. With promising results suggesting stronger and more selective anticancer effects when compared to the Aboriginal remedy, Carica papaya leaf juice warrants further study to explore its activity on other cancer cell lines, as well as investigation to confirm the identity of compounds contributing to its selective effect, particularly those compounds altered by the long heating process applied during the traditional Aboriginal remedy preparation. PMID:26829042

  5. The effect of antifungal hot-water treatments on papaya postharvest quality and activity of pectinmethylesterase and polygalacturonase

    OpenAIRE

    Chvez-Snchez, Ignacio; Carrillo-Lpez, Armando; Vega-Garca, Misael; Yahia, Elhadi M.

    2011-01-01

    The effect of antifungal hot-water treatments (AHWT) at 55C for 0, 3, 6 and 9min on quality attributes and cell-wall enzymatic activity during storage at 25C was investigated in papaya fruit. The total soluble solids (TSS), pH, titratable acidity (TA), firmness and fresh weight loss were not affected, whereas color on skin was negatively affected by the treatments of 6- and 9-min. However, the skin color was not different between the 3-min treated fruit and the untreated fruit during the...

  6. Estudo do processo de extração de papaína a partir do látex do fruto de mamão (Carica papaya L. cv. Maradol Study of the extraction process of papain from the latex of the fruit of papaya (Carica papaya L. cv. Maradol

    Directory of Open Access Journals (Sweden)

    Margarita M Andrade-Mahecha

    2011-07-01

    Full Text Available Neste trabalho foi estudado o processo de extração da papaína presente no látex de frutos de mamão (Carica papaya L. cultivar Maradol. As variáveis estudadas na extração da papaína foram proporção de látex:álcool (1:2.1 e 1:3 e tipo de secagem (à vácuo e por refractance window. As respostas obtidas foram atividade enzimática da enzima e rendimento do processo de extração. O melhor resultado em termos de atividade enzimática e rendimento foi obtido nas condições de secagem à vácuo e proporção látex:álcool de 1:3. A enzima obtida foi caracterizada por testes físico-químicos, microbiológicos e de atividade enzimática e comparada com uma amostra comercial usada como padrão.In this work, we studied the Papain extraction process, from the latex of papaya fruit (Carica papaya L. cv. Maradol. The variables studied in the extraction of papain were: the proportion of latex:alcohol (1:2.1 and 1:3 and type of drying( vacuum and refractance window and the responses obtained in terms of enzymatic activity of the enzyme papain and yield of the extraction process. The best result in terms of enzyme activity and yield was the procedure used to vacuum drying and the proportion latex:alcohol 1:3. The enzyme obtained was characterized by physico-chemical, microbiological and enzymatic activity when compared with a commercial sample used as standard.

  7. A microsatellite library for Carica papaya L. cv. Sunrise solo Marcadores microssatélites para Carica papaya L. cv. Sunrise solo

    Directory of Open Access Journals (Sweden)

    Silvia Correa Santos

    2003-08-01

    Full Text Available In experimental areas of the Education and Researches Ilha Solteira and Jaboticabal UNESP/Campus farms were selected and tagged 20 hermaphrodite plants and 20 feminine of cultivar Sunrise Solo, Improved Sunrise Solo cv.72/12 and Baixinho of Santa Amália.The seeds origined of the selected fruits were cropped to be analysed the self-pollination efficiency and frequency of the sex in the progenies. After that, samples of the young leaf of the matrix plants were colected for the extration of the DNA. It was built five library enriched of microsatellite sequencies, using probes (TCA10, (TC13, (GATA4, (CAC10 e (TGAG8.It was possible the development of the primers only in the library that has utilized the probe (TCA10. This probe allowed the design of 32 primer pairs. From these, 31 presented pattern of unique band in agarose Metaphor and in acrilamide. For primer S36 were observed 2 bands, but with no polymorphism to differentiation in the sexual form at papaya tree culture. However, these primers can be tested, in the futures, in the investigation of the others features in segregated populations of this specie and the related species, germoplasm analysis, cultivars identification, parent evolution and molecular markers for the assisted plant breeding programs.Em áreas experimentais das Fazendas de Ensino e Pesquisa da UNESP/Campus de Ilha Solteira e Jaboticabal foram selecionadas e marcadas 20 plantas hermafroditas e 20 femininas dos cultivares Sunrise Solo, Improved Sunrise Solo cv.72/12 e Baixinho de Santa Amália. As sementes provenientes dos frutos selecionados foram plantadas para analisar-se a eficiência da autofecundação e a freqüência dos sexos nas progênies. Posteriormente, amostras de tecido foliar jovem das plantas matrizes foram coletadas para a extração de DNA. Foram construídas cinco bibliotecas enriquecidas de seqüências microsatélites, utilizando-se as sondas (TCA10, (TC13, (GATA4, (CAC10 e (TGAG8. Foi possível o desenvolvimento de primers somente com a biblioteca que utilizou a sonda (TCA10 . Esta permitiu o desenho de 32 pares de primers. Destes, 31 apresentaram padrão de banda única em agarose Metaphor e em acrilamida. Para o primer S36 foram observadas 2 bandas, mas sem polimorfismo para diferenciação da forma sexual na cultura do mamoeiro. No entanto, estes primers poderão ser testados na investigação de outras características em populações segregantes desta espécie e de espécies afins, análises de germoplasma, identificação de cultivares, evolução parental e marcas em melhoramento assistido.

  8. Expressão transiente do gene gus, sob regulação de quatro promotores, em diferentes tecidos de mamoeiro (Carica papaya L.) e videira (Vitis sp.) Transient expression of the gus gene, under the regulation of four promoters, on different tissues of papaya (Carica papaya l.) and grape (Vitis sp.)

    OpenAIRE

    Adelar Almeida Pinto; Marly Catarina Felipe Coelho; Manoel Teixeira Souza Jr; Miguel Pedro Guerra

    2002-01-01

    O mamoeiro (Carica papaya L.) e a videira (Vitis vinifera L.) destacam-se entre as fruteiras produzidas no Brasil por serem plantadas em quase todo o território nacional e apresentarem importância econômica e social. A tecnologia de produção de organismos geneticamente modificados, também conhecidos como "transgênicos", tem grande potencial de uso no desenvolvimento de fruteiras melhoradas. Porém, questões de propriedade intelectual limitam o uso da engenharia genética por países em desenvolv...

  9. A 1H NMR Investigation of the Interaction between Phenolic Acids Found in Mango (Manguifera indica cv Ataulfo) and Papaya (Carica papaya cv Maradol) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) Free Radicals

    Science.gov (United States)

    López-Martínez, Luis M.; Santacruz-Ortega, Hisila; Navarro, Rosa-Elena; Sotelo-Mundo, Rogerio R.; González-Aguilar, Gustavo A.

    2015-01-01

    The benefits of phenolic acids on human health are very often ascribed to their potential to counteract free radicals to provide antioxidant protection. This potential has been attributed to their acidic chemical structure, which possesses hydroxyl groups in different positions. Phenolic acids can interact between themselves and exhibit an additive, antagonistic or synergistic effect. In this paper, we used 1H NMR to analyze the interactions and mechanisms that are present in major phenolic acids found in mango (gallic, protocatechuic, chlorogenic and vanillic acids) and papaya (caffeic, ferulic and p-coumaric acids), and the DPPH radical was used to evaluate the effect of the antioxidant mixtures. The interactions were found to occur via hydrogen bonds between the -OH and -COOH groups. Moreover, the phenolic acids exhibit two types of mechanisms for the neutralization of the DPPH radical. According to the results, these two mechanisms are Hydrogen Atom Transfer (HAT) and Single Electron Transfer (SET). The ability of the phenolic acid to neutralize the DPPH radical decreases in the following order in mango: gallic > chlorogenic > protocatechuic > vanillic. Moreover, within the acids found in papaya, the order was as follows: caffeic > p-coumaric > ferulic. PMID:26559189

  10. Efectos de la fertilización con nitrógeno en la producción de papaya (Carica papaya L. y en la incidencia de virosis.

    Directory of Open Access Journals (Sweden)

    Vallejo G. Gladys

    1999-06-01

    Full Text Available En un suelo Vertic Aplustoll arcilloso sobre franco esmetítico, isohipertérmico, del Centro de Producción Agropecuaria ACotové@, ubicado en el municipio de Santafé de Antioquia, en la zona de vida bosque seco Tropical (bs-T, se realizó un trabajo para determinar la respuesta de la variedad de papaya AU.N. Cotové@ (Carica papaya L. a la fertilización con N en dosis de 0, 183 y 366 kg/ha, equivalentes a dosis individuales de 5, 5, 10, 15, 25, 25, 25 y 10,10, 20, 30, 50, 50, 50 g de N/planta, respectivamente, fertilizando cada mes durante los primeros 4 meses después del transplante y cada 2 meses posteriormente, durante un período de 9 meses. Se suministraron además dosis constantes de P, K, S, Fe, Mn, Zn, y B. Se utilizó un diseño de bloques al azar con cuatro repeticiones y 10 plantas por unidad experimental, con distancias entre plantas de 2 m y entre surcos de 3m.

  11. Revista Chilena de Cirugía: índice de publicación según procedencia de los artículos Summ affiliation of authors publishing in Revista Chilena de Cirugía

    Directory of Open Access Journals (Sweden)

    MANUEL VIAL G

    2008-02-01

    Full Text Available Introducción: Una de las variables que se analiza en los estudios bibliométricos es la procedencia de las publicaciones. Este tipo de análisis ha permitido cuantificar el impacto que tienen las distintas universidades o centros hospitalarios en el total de publicaciones de una revista en particular. El objetivo de este estudio es determinar una eventual asociación entre el índice de publicación y la procedencia de los artículos publicados en la Revista Chilena de Cirugía (Rev Chil Cir. Material y método: Estudio biblio-métrico. Se analizaron todos los artículos publicados en la Rev Chil Cir entre los años 2002 y 2006. Los artículos se categorizaron de acuerdo al año de publicación, el tipo de artículo, región del país que originó el artículo y la filiación universitaria manifestada en cada artículo. Se calculó la tasa de publicación, el promedio anual y general de publicación, y la tasa de publicación anual promedio (TPAP. Posteriormente, se aplicó estadística analítica para determinar diferencias entre grupos, utilizando T de Student, ANOVA y prueba de comparaciones múltiples de Duncan. Resultados: En el periodo estudiado, se publicaron 545 artículos científicos, con un promedio de 18,2 artículos por número y 109 artículos por volumen. El 67,9 % fue generado por la Región Metropolitana (RM, el 10,8 % por la VIII región y el 7,8 % por la IX región. Solo en un 64,8 % de las publicaciones queda de manifiesto alguna filiación universitaria. Las mayores TPAP correspondieron a los Capítulos IX y VIII Región con 2,3 + 0,8 y 1,8 + 0,2 publicaciones/año x 10 miembros (p Background: The analysis of author affiliation of published manuscripts allows an assessment of the impact that different universities or hospitals have on a determinate journal. Aim: To analyze author affiliation of manuscripts published in Revista Chilena de Cirugía. Material and methods: All manuscripts published in the journal between 2002 and 2006, were analyzed. The affiliation of authors, the year of publication and the region of Chile in which the manuscript was generated, were recorded. The publication rate, annual average were calculated. The association of authors to specific regional subsidiaries of the Chilean Society of Surgeons was also recorded and the average annual publication rates per regional subsidiary, were calculated. Resu/fs; During the study period 545 manuscripts were published, with a mean of 18 manuscripts per issue and 109 per volume. Sixty eight percent of manuscripts came from the metropolitan region, 11 % from the eight region and 8 % from the ninth region. In only 65 % of manuscripts, authors had an university affiliation. The higher average annual publication rates were observed in the subsidiaries of the eighth and ninth region, with 2.3 + 0.8 and 1.8 + 0.2 publications per year per 10 members, respectively. Conclusions: An important proportion of manuscripts have authors without university affiliation. The higher average annual publication rates come from regional subsidiaries of the Chilean Society of Surgeons

  12. Registro y transcripción testimonial en la poesía chilena actual: Lihn, Zurita Testimonial register and transcription in present day Chilean poetry: Lihn, Zurita

    Directory of Open Access Journals (Sweden)

    Oscar Galindo V

    2003-01-01

    Full Text Available El artículo analiza, en el contexto de los procesos de mutación disciplinaria que caracterizan la poesía chilena e hispanoamericana actual, la incorporación del procedimiento de registro y transcripción testimonial como estrategia de producción poética en la escritura de Enrique Lihn y Raúl Zurita.The article analizes, in the context of the discipline mutations that characterize Chilean and Hispanoamerican poetry today, the incorporation of the testimonial register and transcription as poetic production in the work of Enrique Lihn and Raúl Zurita.

  13. Abordajes Frente al Terremoto y Tsunami del 27 de Febrero del 2010: Experiencia de la Sociedad Chilena de Psicología Clínica (SCPC)

    OpenAIRE

    Jade Ortiz Barrera; Caterina Manzo García

    2010-01-01

    En el presente artículo se describe de forma sintética las actividades realizadas por la Sociedad Chilena de Psicología Clínica posteriormente al terremoto y tsunami del 27 de Febrero del 2010, en la zona sur y centro de Chile. Dentro de éstas se presenta la coordinación de capacitaciones a psicólogos y equipos de salud que trabajaron en terreno, preparación de material escrito que fue entregado a la población en la ciudad de Santiago y el trabajo conjunto con la Policía de Investigaciones en...

  14. Regional journals in medicine and public health: A look to the future upon the indexing of the Revista Chilena de Infectologa

    Directory of Open Access Journals (Sweden)

    STEN H. VERMUND

    2005-03-01

    Full Text Available Revista Chilena de Infectologa has recently been accepted for index listing in the Index Medicus via the MEDLINE data base. This breakthrough prompts reflection on the nature of regionally focused subspecialty journals and what such a listing means for better dissemination of scientific and medical discoveries reported in such journals. There is a conscientious global effort at present to better represent regional journals in indexing systems and to make access easier for persons in less wealthy nations to medical and public health information via the internet. These trends are reviewed and discussed in the context of Chile's national medical and public health literature

  15. Interdisciplinariedad, interculturalidad y canon en la poesa chilena e hispanoamericana actual / Interdisciplinarity, interculturality and canon of the present Chilean and Latin American poetry

    Scientific Electronic Library Online (English)

    Ivn, Carrasco M.

    Full Text Available En este trabajo se postula la existencia de zonas de indeterminacin genrica y textual en el discurso contemporneo, que ponen en crisis la estabilidad del canon literario; esta indefinicin es provocada, en el caso especfico de la poesa chilena e hispanoamericana de los ltimos aos, por estrate [...] gias de mutacin interdisciplinaria y de hibridismo cultural. Abstract in english This work postulates the existence of undefined textual and generic areas in contemporary discourse that put the literary canon in crisis; the indeterminacy has been brought about by interdisciplinary mutation and cultural hybridation in Chilean and Hispanic American poetry in the last few years. [...

  16. La oposición a la dictadura de Miguel Primo de Rivera a través de la prensa mercurial chilena (1923-1930)

    OpenAIRE

    Mario Valdés Urrutia

    2011-01-01

    Este artículo examina algunos aspectos del accionar opositor al gobierno de Miguel Primo de Rivera desde una mirada sudamericana: el diario El Mercurio, influyente periódico en la sociedad chilena. Las reacciones frente al alzamiento primorriverista de 1923; la crítica política junto a algunos intentos de defenestrar la Dictadura;  y el  rechazo al proyecto de institucionalización autoritaria y antiliberal, son los temas abordados.Palabras claves: Primo de Rivera – Dictadura – Oposición – Vis...

  17. La metáfora en la Lengua de Señas Chilena. Una aproximación desde la psicolingüística cognitiva

    OpenAIRE

    Acuña Robertson, Ximena

    2016-01-01

    Este estudio se propone conocer la manera como se construyen y se interpretan los significados en la Lengua de Señas Chilena. Mediante el análisis de expresiones lingüísticas, se establece un conjunto de redes conceptuales y metáforas cognitivas, a través de lo cual se describe la conceptualización de dominios cognitivos relativos a la comunicación, las emociones, el tiempo y la actividad intelectual. Se enfoca sobre operaciones que subyacen, sus dominios y en qué forma esto se refleja en el ...

  18. EXPORTACIÓN DE MADERA ASERRADA DE CONÍFERA CHILENA: UN ANÁLISIS DE SU COMPETITIVIDAD CHILEAN EXPORT OF SOFTWOOD SAWNWOOD: AN ANALYSIS OF ITS COMPETITIVENESS

    OpenAIRE

    Mauricio Ponce D.; Manuel Contreras G; Marcia Vásquez S

    2007-01-01

    Se analiza la competitividad en los principales mercados de las exportaciones chilenas de madera aserrada de pino radiata en el período 1994-2004 (Contreras, 2006). Se aplica la metodología "Competitive Análisis of Nation" (CAN), creada por la Comisión Económica para América Latina y el Caribe (CEPAL), que permite obtener una visión dinámica de las situaciones de competitividad del producto en los mercados estudiados. Los resultados se compararon con un estudio similar del periodo 1987 a 1997...

  19. EMBARAZO ECTÓPICO: FACTORES DE RIESGO Y CARACTERÍSTICAS CLÍNICAS DE LA ENFERMEDAD EN UN GRUPO DE MUJERES CHILENAS

    OpenAIRE

    María Teresa Urrutia S; Lauren Poupin B; Paula Alarcón A; Magdalena Rodríguez C; Loreto Stuven R

    2007-01-01

    Antecedentes: El embarazo ectópico (EE) es una de las principales causa de muerte materna durante el primer trimestre del embarazo. En las últimas décadas se registra un aumento de su incidencia. Objetivo: Establecer los factores de riesgo asociados a esta patología, en una población de mujeres chilenas. Método: Se estudió a 50 mujeres con EE y se compararon con 100 puérperas en el mismo período. Resultados: Los factores de riesgo significativos para EE fueron: antecedentes de cirugía ginecol...

  20. AVALIAO AGRONMICA DE LINHAGENS E HBRIDOS DE MAMOEIRO / AGRONOMIC EVALUATION OF PAPAYA LINES AND HYBRIDS

    Scientific Electronic Library Online (English)

    JORGE LUIZ LOYOLA, DANTAS; RANGEL SALES, LUCENA; SANDIELLE ARAJO, VILAS BOAS.

    2015-03-01

    Full Text Available RESUMO Objetivou-se avaliar o potencial agronmico de hbridos e linhagens de mamoeiros sintetizados pela Embrapa Mandioca e Fruticultura, visando a identificar e a selecionar gentipos com boas caractersticas agronmicas, passveis de explorao no programa de melhoramento gentico e uso direto n [...] o sistema de produo de mamo. O trabalho foi conduzido em rea do Setor de Campos Experimentais da Embrapa Mandioca e Fruticultura, em Cruz das Almas - BA. Foram avaliados 22 gentipos (oito linhagens, doze hbridos e duas testemunhas: Sunrise Solo e Tainung n 1) em delineamento de blocos casualizados, com quatro repeties, contendo seis plantaspor parcela, totalizando 528 plantas no ensaio. Para a maioria dos caracteres, observaram-se diferenas significativas a 1% e 5% de probabilidade, exceto para dimetro do caule (DC). A linhagem L60 e o hbrido H10.60 destacaram-se com as menores mdias para a altura da planta (AP) e a altura de insero dos primeiros frutos (AIPF), e no diferiram estatisticamente entre si. As linhagens L10 e L72, e os hbridos H10.26, H10.60 e H10.72 apresentaram maiores mdias de nmeros de frutos comerciais (NFC) que a testemunha Sunrise Solo. Para produtividade (PROD), observou-se ampla variabilidade entre os tratamentos, sendo que 75% dos hbridos e linhagens superaram as testemunhas. Mais de 50% dos gentipos apresentaram mdias de slidos solveis (SS) superiores a 12Brix. Com o objetivo de reunir caractersticas favorveis para o desenvolvimento de novas variedades, as linhagens e os hbridos que apresentaram comportamentos satisfatrios para os principais caracteres foram: L60 e H10.60, para AIPF e AP; L10, L72, H10.26, H10.60 e H10.72, para NFC; L10, H10.72, H26.72, H33.56 e H36.45 para PROD; e L26, L36, H10.26, H10.60 e H60.72, para SS. Abstract in english ABSTRACT This study aimed to evaluate the agronomic potential of papaya hybrids synthesized by Embrapa Cassava & Fruits, to identify and select genotypes with good agronomic characteristics, exploitable in the breeding program and direct use in the papaya production system. This study was carried o [...] ut in the Experimental Field Sector of Embrapa Cassava & Fruits, Cruz das Almas - BA. It was evaluated 22 genotypes (eight lines, twelve hybrids and two witnesses: Sunrise Solo and Tainung no 1) in a randomized block design with four replications of six plants perplot, totalizing 528 plants. For most characters significant differences were observed at 1% and 5% probability, except for stem diameter (SD). The line L60 and H10.60 stood out with the lowest averages for plant height (PH) and insertion height of the first fruits (IHFF), and were not statistically different. Lines L10 and L72, and the hybrids H10.26, H10.60 and H10.72 showed higher averages of numbers of commercial fruits (NCF) than the witness Sunrise Solo. For yield, it was observed wide variability among treatments, with 75% of lines and hybrids overcame the witnesses. Over 50% of the genotypes showed soluble solids (SS) averages above 12 Brix. Aiming to associate favorable characteristics for development of new varieties, lines and hybrids that showed satisfactory behavior to the main characters were: L60 and H10.60 to IHFF and PH; L10, L72, H10.26, H10.60 and H10.72, for NCF; L10, H10.72, H26.72, H33.56 and H36.45, for productivity (PROD), and L26, L36, H10.26, H10.60 and H60.72, for SS.