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Sample records for papaya chilena vasconcellea

  1. Papaya Lethal Yellowing Virus (PLYV) Infects Vasconcellea cauliflora

    Amaral, P.P.R.; Resende, de R.O.; Souza, M.T.

    2006-01-01

    Papaya lethal yellowing virus (PLYV) é um dos três vírus descritos infectando mamoeiros (Carica papaya L.) no Brasil. Vasconcellea cauliflora (Jacq.) A. DC., antes denominada de Carica cauliflora (Jacq.), é uma reconhecida fonte de resistência natural ao Papaya ringspot virus (PRSV), causador da "Ma

  2. Deshidratación osmótica de la papaya chilena (Vasconcellea pubescens e influencia de la temperatura y concentración de la solución sobre la cinética de transferencia de materia Desidratação osmótica do mamão chileno (Vasconcellea pubescens e influência da temperatura e concentração da solução sobre a cinética de transferência de matéria

    Antonio Vega-Gálvez

    2007-09-01

    Full Text Available El objetivo de este trabajo fue estudiar la cinética de deshidratación osmótica de la papaya chilena (Vasconcellea pubescens, utilizando dos variables experimentales: la temperatura (30, 40, 50 °C y concentración (40, 50 y 60% de la solución osmótica. Se determinó la variación de masa total, masa de agua y masa de sólidos solubles y se modeló con las ecuaciones difusionales, además en esta investigación se consideró y utilizó una modificación de la ecuación propuesta por Biswal-Bozorgmehr. Estos modelos fueron evaluados por medio del coeficiente de regresión lineal, suma de errores cuadrados, raíz media de los errores cuadrados y Chi-cuadrado. Al comparar los valores experimentales con los calculados, se demostró que el modelo Biswal-Bozorgmehr modificado obtuvo mejor ajuste sobre la variación de masa de agua y sólidos solubles. Mediante la ecuación de Arrhenius se analizó el efecto de la temperatura sobre los parámetros cinéticos (Kw y Kss y difusividad efectiva de agua (Dwe y sólidos solubles (Dsse. De acuerdo al diseño factorial, se observó que la temperatura no influyó sobre la difusividad efectiva de agua y de sólidos, pero sí la concentración de la solución osmótica. La mejor condición de salida de agua y ganancia de sólidos solubles durante la deshidratación osmótica de la papaya chilena fue a 30 °C con una concentración del 60%.O objetivo deste trabalho foi estudar a cinética de desidratação osmótica do mamão chileno (Vasconcellea pubescens utilizando duas variáveis experimentais: temperatura (30, 40 e 50 °C e concentração (40, 50 e 60% da solução osmótica. Para este estudo, determinou-se a variação de massa total, de massa de água, de massa de sólidos solúveis, modeladas com as equações difusionais. Nesta investigação, também se considera e se usa uma modificação da equação proposta por Biswal-Bozorgmehr. Estes modelos foram avaliados através do coeficiente de regress

  3. Extraction Techniques for Bioactive Compounds and Antioxidant Capacity Determination of Chilean Papaya (Vasconcellea pubescens) Fruit

    Elsa Uribe; Alvaro Delgadillo; Claudia Giovagnoli-Vicuña; Issis Quispe-Fuentes; Liliana Zura-Bravo

    2015-01-01

    The aim of this work was to assess and compare different extraction methods by using high hydrostatic pressure (HHPE), ultrasound (UE), agitation (AE), and their combinations for the extraction of bioactive compounds of Chilean papaya. Extract antioxidant capacity was evaluated by three methods (i.e., DPPH, FRAP, and Voltammetry) and phenolic compounds and vitamin C were determined by HPLC. Papaya sample extraction was performed by HHPE at 500 MPa for 10 min and UE and AE for 30 min, respecti...

  4. Papaya

    Papaya is a plant. The leaves are used to make medicine. Papaya is used for preventing and treating gastrointestinal tract ... of the lymphatic system caused by parasitic worms. Papaya contains a chemical called papain, which is commonly ...

  5. Intestinal obstruction due to Vasconcellea seeds: Report of three cases and literature review

    Montoya-González, Juliana; Vasco, Alejandra; Hoyos-Figueroa, Francisco; Osorio-Cadavid, Natalia; Marín-Agudelo, Alejandro; Jaramillo-Bustamante, Juan Camilo

    2015-01-01

    Vasconcellea spp., is a species of Andean papaya commonly cultivated in rural communities close to Medellín, Colombia. Due to the pleasant and sweet flavor of its fruits, children frequently ingest its seeds accidentally. After ingestion, the seeds are engaged in the colonic lumen and block the exit of stools, causing pain and bloating, and promoting bacterial translocation. Diagnosis is based on clinical history and rectal examination. Treatment depends on the degree of local and systemic in...

  6. Palynology of Carica and Vasconcellea (Caricaceae Palinología de Carica y Vasconcellea (Caricaceae

    Lagos Túlio César

    2006-09-01

    Full Text Available

    Palynology of Carica and Vasconcellea (Caricaceae. The pollen of C. papaya and agreements of Vasconcellea cauliflora, V. cundinamarcensis, V. crassipetala, V. goudotiana, V. x heilbornii var. chrysopetala, V. longiflora and V. sphaerocarpa collected in the Colombian Coffee Growing Zone, using the technique of acetolisis for optic microscopy and the fixation procedure with glutaraldehide, dehydration and ionization with gold-palade, for scanning electronic microscopy was described. The pollen grains were characterized using descriptors, which include the characters of taxonomic value for pollen identification, genetically determined. The most important are the number, position and character of the aperture (NPC and exine ornamentation and stratification. The pollen is of medium size for both genera, tricolporate, zonoaperturate, prolate-spheroid to subprolate, isopolar radial symmetry, tectate, dug, foveolate, with columelas. These characters have demonstrated a great contribution to the taxonomy of Caricaceae because the cluster analysis allowed distinguish very well the two genera.

    Se describe el polen de C. papaya y accesiones de Vasconcellea cauliflora, V. cundinamarcensis, V. crassipetala, V. goudotiana, V. x heilbornii var. chrysopetala, V. longiflora y V. sphaerocarpa recolectadas en la Zona Cafetera de Colombia, empleando la técnica de acetólisis para microscopía óptica y el procedimiento de fijación con glutaraldehído, deshidratación e ionización con oro paladio, para microscopía electrónica de barrido (MEB. Se caracterizaron los granos de polen por medio de una lista de descriptores que incluyeron los caracteres de valor taxonómico para identi

  7. Citogenética de especies de Vasconcellea (Caricaceae

    Creucí Maria Caetano

    2008-12-01

    Full Text Available Conocer aspectos básicos de la citogenética de las papayas de montaña o papayuelas, especies del género Vasconcellea (Caricaceae originarias de Ecuador y Colombia, fue el objetivo del trabajo. Botones florales se fijaron en etanol-acético 3:1 por 24h, luego se transfirieron a nueva solución y se almacenaron en frío. Los microsporocitos y los granos de polen se tiñieron con acetocarmín (1%. Se evaluaron las fases de meiosis y la viabilidad de polen para describir el comportamiento cromosómico. Se encontró que: 1 todas las seis especies estudiadas fueron diploides (2n=2x=18; 2 se describió el número cromosómico para V. sphaerocarpa, V. longiflora y V. palandensis (2n=18; 3 el grado de asimetría de los complementos cromosómicos indicó un proceso de evolución; 4 la presencia de numerosos NOR en V. sphaerocarpa está relacionada con los micronucleolos y corroboró el posible origen híbrido; y 5 V. cauliflora (42.98% y V. cundinamarcensis (47.93% presentaron la más baja viabilidad polínica.

  8. Intestinal obstruction due to Vasconcellea seeds: Report of three cases and literature review

    Montoya-González, Juliana

    2015-07-01

    Full Text Available Vasconcellea spp., is a species of Andean papaya commonly cultivated in rural communities close to Medellín, Colombia. Due to the pleasant and sweet flavor of its fruits, children frequently ingest its seeds accidentally. After ingestion, the seeds are engaged in the colonic lumen and block the exit of stools, causing pain and bloating, and promoting bacterial translocation. Diagnosis is based on clinical history and rectal examination. Treatment depends on the degree of local and systemic involvement and includes disimpaction of rectal contents under general anesthesia and colonic washes with 0.9% saline solution. In severe cases derivative colostomy has been required. In the literature there are no reports of intestinal obstruction due to Vasconcellea seeds, possibly because it has been mistaken for seeds of the genus Carica. In this article, three cases treated at pediatric services in Medellín, Colombia, in 2012 and 2013 are described. We note that this is a rarely suspected disease, leading to late diagnosis and potential catastrophic consequences. It is important to educate people to prevent the ingestion of the seeds.

  9. Citogenética de especies de Vasconcellea (Caricaceae

    Caetano Creucí María

    2008-12-01

    Full Text Available Conocer aspectos básicos de la citogenética de las papayas de montaña o papayuelas, especies del género Vasconcellea (Caricaceae originarias de Ecuador y Colombia, fue el objetivo del trabajo. Botones florales se fijaron en etanol-acético 3:1 por 24h, luego se transfirieron a nueva solución y se almacenaron en frío. Los microsporocitos y los granos de polen se tiñieron con acetocarmín (1%. Se evaluaron las fases de meiosis y la viabilidad de polen para describir el comportamiento cromosómico. Se encontró que: 1 todas las seis especies estudiadas fueron diploides (2n=2x=18; 2 se describió el número cromosómico para V. sphaerocarpa, V. longiflora y V. palandensis (2n=18; 3 el grado de asimetría de los complementos cromosómicos indicó un proceso de evolución; 4 la presencia de numerosos NOR en V. sphaerocarpa está relacionada con los micronucleolos y corroboró el posible origen híbrido; y 5 V. cauliflora (42.98% y V. cundinamarcensis (47.93% presentaron la más baja viabilidad polínica.

  10. Mejoramiento de la germinación, control de la hiperhidricidad y formación de brotes en Vasconcellea stipulata Badillo

    Rosa Armijos González

    2015-11-01

    Full Text Available Vasconcellea stipulata has great commercial importance because of its enzymatic activity and as a source for genetic improvement of papaya since it is resistant to the papaya ringspot virus. However, due to its low regeneration by seeds and limited knowledge of its genetic and pharmaceutical properties, this species is not widely cultivated. For propagation, in vitro culture of seeds has been used to address this problem, but hyperhydricity, a physiological disorder, mainly expressed in the developing embryonic axis and specifically associated with this species, is a significant constraint. In order to obtain elite material for culture of V. stipulata, the aim of this work was to increase germination, to control hyperhydricity in embryos and to evaluate the potential to induce morphogenic responses, i.e., shoot formation. Our results showed that it is possible to increase germination up to 53% under in vitro conditions within a short period in the presence of hydrogen peroxide. In addition, hyperhydricity was significantly reduced (50% in vitro when gibberellic acid concentrations were included on a 1/2 Nitsch and Nitsch nutrient medium, resulting in approximately 80% recovery of viable seedlings. Finally, other plant growth regulators were evaluated and found to trigger shoot formation in axillary buds as well as induce the formation of callus in leaf sections derived of seedlings.

  11. A dated phylogeny of the papaya family (Caricaceae) reveals the crop's closest relatives and the family's biogeographic history.

    Carvalho, Fernanda Antunes; Renner, Susanne S

    2012-10-01

    Papaya (Carica papaya) is a crop of great economic importance, and the species was among the first plants to have its genome sequenced. However, there has never been a complete species-level phylogeny for the Caricaceae, and the crop's closest relatives are therefore unknown. We investigated the evolution of the Caricaceae based on sequences from all species and genera, the monospecific Carica, African Cylicomorpha with two species, South American Jacaratia and Vasconcellea with together c. 28 species, and Mexican/Guatemalan Jarilla and Horovitzia with four species. Most Caricaceae are trees or shrubs; the species of Jarilla, however, are herbaceous. We generated a matrix of 4711 nuclear and plastid DNA characters and used maximum likelihood (ML) and Bayesian analysis to infer species relationships, rooting trees on the Moringaceae. Divergence times were estimated under relaxed and strict molecular clocks, using different subsets of the data. Ancestral area reconstruction relied on a ML approach. The deepest split in the Caricaceae occurred during the Late Eocene, when the ancestor of the Neotropical clade arrived from Africa. In South America, major diversification events coincide with the Miocene northern Andean uplift and the initial phase of the tectonic collision between South America and Panama resulting in the Panamanian land bridge. Carica papaya is sister to Jarilla/Horovitzia, and all three diverged from South American Caricaceae in the Oligocene, 27 (22-33) Ma ago, coincident with the early stages of the formation of the Panamanian Isthmus. The discovery that C. papaya is closest to a clade of herbaceous or thin-stemmed species has implications for plant breeders who have so far tried to cross papaya only with woody highland papayas (Vasconcellea). PMID:22659516

  12. Sex Determination in Papaya

    Sex determination is an intriguing system in trioecious papaya. Over the past seven decades various hypotheses, based on the knowledge and information available at the time, have been proposed to explain the genetics of the papaya's sex determination. These include a single gene with three alleles...

  13. BASKETFUL BENEFITS OF PAPAYA

    Parle Milind

    2011-07-01

    Full Text Available Papaya (Carica papaya linn is well known for its exceptional nutritional and medicinal properties throughout the world. From the times immemorial, the whole Papaya plant including its leaves, seeds, ripe and unripe fruits and their juice is used as a traditional medicine. The fruit has a large oval shape, yellowish-green skin and yellow flesh. Nowadays, Papaya is considered as a Nutraceutical fruit due to its multi-faceted medicinal properties. The prominent medicinal properties of Papaya include Anti-fertility, Uterotonic, Diuretic, Anti-hypertensive, Hypolipidemic, Anti-helmintic, Wound-healing, Anti-fungal, Anti-bacterial, Anti-tumor and Free radical scavenging activities. Phytochemically, the whole plant contains enzymes (Papain, carotenoids, alkaloids, monoterpenoids, flavonoids, minerals and vitamins. In the present review article, a humble attempt is made to compile all the strange facts available about this tasty fruit. This tasty fruit of Papaya is popular among family members of all ages for the delicious dishes derived from it.

  14. Post-irradiation identification of papaya ( Carica papaya L.) fruit

    Chatterjee, Suchandra; Variyar, Prasad S.; Sharma, Arun

    2012-03-01

    Impact of radiation processing on the volatile essential oil profile of papaya ( Carica papaya) was investigated. Gamma-radiation processing resulted in the appearance of a new peak in the GLC profile that was identified as phenol. The observed dose dependent increase in phenol content suggested possible use of this compound as a marker for radiation processed papaya.

  15. Papaya: environment and crop physiology

    Papaya (Carica papaya L.) is a principal horticultural crop of tropical and subtropical regions. Knowledge of how papaya responds to environmental factors provides a scientific basis for the development of management strategies to optimize fruit yield and quality. A better understanding of genotyp...

  16. Papaya ringspot virus (Potyviridae)

    Papaya ringspot virus, a member of the family Potyviridae, is single stranded RNA plant virus with a monocistronic genome of about 10,326 nucleotides that is expressed via a large polyprotein subsequently cleaved into functional proteins. It causes severe damage on cucurbit crops such as squash and...

  17. Características Físico-Químicas del Látex de Papayuelo (Vasconcellea cundinamarcensis Badillo, Caricaceae Physico-Chemical Characteristics of Papayuelo Latex (Vasconcellea Cundinamarcensis Badillo, Caricaceae

    Leslie V Vidal

    2009-01-01

    Full Text Available Se determinó el rendimiento y la actividad enzimática de la papaína presente en el látex de papayuelo (Vasconcellea cundinamarcensis, cultivado en Cobquecura, VIII región del Bío-Bío, Chile, durante un año. Entre enero y diciembre, las plantas, frutos y látex, obtenidos de frutos inmaduros, fueron caracterizados con el propósito de conocer el comportamiento del cultivo a través de las estaciones del año, y comprender las posibles relaciones entre los parámetros estudiados. El diseño experimental fue completamente aleatorio y los datos se procesaron mediante un ANDEVA y prueba de rangos múltiples de Duncan. Se encontró diferencia estadística significativa entre estaciones en cuanto al desarrollo de la planta y sus características. Los mayores rendimientos de látex se obtuvieron en la estación de primavera, mientras que la actividad enzimática se mantuvo constante durante el año de estudio.A study to determine the yield and enzymatic activity of papain present in the latex obtained from papayuelo fruit (Vasconcellea cundinamarcensis cultivated in Cobquecura, VIII Bío-Bío Region in Chile, during one year was done. Between January and December the papayuelo plants, fruits and latex obtained from unripe fruits were characterized. The objective of the study was to know the species behavior through the seasons and the relation between the parameters studied. The experimental design was completely random, and the data were processed through ANOVA and Duncan’s multiple range test. A significant statistical difference in plant growth and plan characteristics between seasons was found. The higher latex yields were obtained in spring, whereas the enzymatic activity was constant through the year.

  18. Post-irradiation identification of papaya (Carica papaya L.) fruit

    Impact of radiation processing on the volatile essential oil profile of papaya (Carica papaya) was investigated. Gamma-radiation processing resulted in the appearance of a new peak in the GLC profile that was identified as phenol. The observed dose dependent increase in phenol content suggested possible use of this compound as a marker for radiation processed papaya. - Highlights: ► Effect of γ-irradiation on the essential oil profile of papaya is demonstrated. ► γ-Irradiation resulted in a dose dependent increase in a new peak, phenol. ► Phenol formed in the volatile oil is proposed as a new marker of irradiated food. ► Content of phenol remained unchanged during the entire storage period.

  19. TRANSGENIC PAPAYA: A CASE FOR MANAGING RISKS OF PAPAYA RINGSPOT VIRUS IN HAWAII

    In May 1992, papaya ringspot virus (PRSV was detected in the Puna district of Hawaii Island, the main papaya growing region of the state of Hawaii. By 1994 Hawaii's papaya industry was facing devastating damage from PRSV. Efforts to develop resistant transgenic papaya were started in the mid 1980s...

  20. Las franjas electorales en la experiencia chilena

    Juan Ignacio García Rodríguez

    2013-07-01

    Full Text Available Expone la experiencia chilena sobre el establecimiento y regulación de las franjas electorales, para lo cual analiza la constitucionalidad de la franja, los principios involucrados, las disposiciones regulatorias, el papel del Consejo Nacional de Televisión, la jurisprudencia que sobre el tema ha sido emitida por el Tribunal Calificador de Elecciones y los retos que el sistema de franjas electorales enfrenta a futuro como lo es su aplicación a las elecciones primarias de los partidos políticos.

  1. La guerra en la dramaturgia chilena

    Faúndez Carreño, Tania

    2014-01-01

    La guerra se ha constituido un tema constante en la dramaturgia chilena, la cual a su vez se ha vinculado estrechamente con la identidad nacional. La pulsión de la guerra, con sus mecanismos discursivos institucionales (histórico, político, cultural, militar, nacional), sus ondulaciones y su dificultad misma de la representación sobre la escena han resonado en la dramaturgia patria desde la consolidación de la República en el siglo XIX (1817) hasta la apoteósica celebración del Bicentenario n...

  2. Improving Tenderness of Spent Layer Hens Meat Using Papaya Leaves (Carica papaya)

    H. O. Abdalla, N. N. A. Ali, F. S. Siddig and S. A. M. Ali*

    2013-01-01

    Two experiments were performed to study the use of papaya leaves as a meat tenderizer. The first experiment was to evaluate the effect of papaya dry leaves added to hen’s diet before slaughter. Spent hens (n=48) were used, half of them were fed a concentrate ration containing10% dried papaya leaves powder (DPLP) while others received layer ration (Control), for 10 days. The second experiment involved a comparison between papaya leaves juice (PLJ), fresh papaya leaves (FPL) and vinegar soluti...

  3. Complete Genome Sequence of Papaya Ringspot Virus Isolated from Genetically Modified Papaya in Hainan Island, China.

    Zhao, Guangyuan; Yan, Pu; Shen, Wentao; Tuo, Decai; Li, Xiaoying; Zhou, Peng

    2015-01-01

    The complete genome sequence (10,326 nucleotides) of a papaya ringspot virus isolate infecting genetically modified papaya in Hainan Island of China was determined through reverse transcription (RT)-PCR. The virus shares 92% nucleotide sequence identity with the isolate that is unable to infect PRSV-resistant transgenic papaya. PMID:26358610

  4. Allergenicity assessment of the Papaya ringspot virus coat protein expressed in transgenic Rainbow papaya

    The virus-resistant, transgenic commercial papaya cultivars Rainbow and SunUp (Carica papaya L.) have been consumed locally in Hawaii and elsewhere in the mainland US and Canada since their release to planters in Hawaii in 1998. These cultivars are derived from transgenic papaya line 55-1 and carry ...

  5. Managing papaya ringspot virus: Impact of grass barriers on alate aphid immigration into papaya orchards

    Papaya ringspot virus, transmitted by alate aphids, is the most limiting factor of papaya production in the Caribbean region. Although there are transgenic papaya varieties that provide protection from this virus, these varieties are effective only in certain regions against certain strains of the v...

  6. Complete Genome Sequence of Papaya Ringspot Virus Isolated from Genetically Modified Papaya in Hainan Island, China

    Zhao, Guangyuan; Yan, Pu; Shen, Wentao; Tuo, Decai; Li, Xiaoying; Zhou, Peng

    2015-01-01

    The complete genome sequence (10,326 nucleotides) of a papaya ringspot virus isolate infecting genetically modified papaya in Hainan Island of China was determined through reverse transcription (RT)-PCR. The virus shares 92% nucleotide sequence identity with the isolate that is unable to infect PRSV-resistant transgenic papaya.

  7. Physical-chemical analyses of irradiated papayas (Carica papaya L.)

    Camargo, R.J. [Radiation Technology Center, IPEN-CNEN/SP, Av. Lineu Prestes, 2242, 05508-900, Sao Paulo, SP (Brazil); Tadini, C.C. [Chemical Engineering Department, Escola Politecnica, Sao Paulo University, P.O. Box 61548, 05424-970, SP (Brazil); Sabato, S.F. [Radiation Technology Center, IPEN-CNEN/SP, Av. Lineu Prestes, 2242, 05508-900, Sao Paulo, SP (Brazil)], E-mail: sfsabato@ipen.br

    2007-11-15

    Papaya is cultivated in Espirito Santo State/Brazil and as it stands up to irradiation, it is important to validate this technology, since it is already being applied in some countries. Penetration energy, ratio (relation between soluble solids and titrable acidity) and skin color were evaluated to verify the influence of four different doses of irradiation (0.0, 0.5, 0.75 and 1.00 kGy) on papayas, during 21 days. As a result for the skin color and the penetration energy, it was found that in the first days after irradiation, these variables increased with increase in radiation dose; however, after a time lapse, the tendency inverted and the irradiated fruits had a slower ripening process. For the ratio, a very important variable that it is responsible for the fruit taste, no difference was found between irradiated and the control fruit. Color and texture measurements are dependent on the storage temperature.

  8. Physical-chemical analyses of irradiated papayas ( Carica papaya L.)

    Camargo, R. J.; Tadini, C. C.; Sabato, S. F.

    2007-11-01

    Papaya is cultivated in Espírito Santo State/Brazil and as it stands up to irradiation, it is important to validate this technology, since it is already being applied in some countries. Penetration energy, ratio (relation between soluble solids and titrable acidity) and skin color were evaluated to verify the influence of four different doses of irradiation (0.0, 0.5, 0.75 and 1.00 kGy) on papayas, during 21 days. As a result for the skin color and the penetration energy, it was found that in the first days after irradiation, these variables increased with increase in radiation dose; however, after a time lapse, the tendency inverted and the irradiated fruits had a slower ripening process. For the ratio, a very important variable that it is responsible for the fruit taste, no difference was found between irradiated and the control fruit. Color and texture measurements are dependent on the storage temperature.

  9. Physical-chemical analyses of irradiated papayas (Carica papaya L.)

    Papaya is cultivated in Espirito Santo State/Brazil and as it stands up to irradiation, it is important to validate this technology, since it is already being applied in some countries. Penetration energy, ratio (relation between soluble solids and titrable acidity) and skin color were evaluated to verify the influence of four different doses of irradiation (0.0, 0.5, 0.75 and 1.00 kGy) on papayas, during 21 days. As a result for the skin color and the penetration energy, it was found that in the first days after irradiation, these variables increased with increase in radiation dose; however, after a time lapse, the tendency inverted and the irradiated fruits had a slower ripening process. For the ratio, a very important variable that it is responsible for the fruit taste, no difference was found between irradiated and the control fruit. Color and texture measurements are dependent on the storage temperature

  10. La izquierda chilena contemparánea

    Manuel Antonio Garretón

    2006-01-01

    Full Text Available En este artículo, el autor examina las particularidades de la izquierda chilena para entender su papel en los gobiernos democráticos desde los años 70. El autor parte de la premisa de la existencia, durante los años 90, de dos izquierdas: una, el socialismo dentro de la coalición; la otra, el comunismo fuera de ella. En el trabajo se sostiene que la izquierda socialista ha sido el principal motor de la coalición y su papel específico se ha concentrado en los temas socio-económicos ligados a la igualdad, a la justicia, a los derechos humanos y a las propuestas culturales de tipo progresista. Asimismo, se analizan las particularidades esenciales de los gobiernos democráticos chilenos y se examinan los temas y las tareas pendientes. El artículo concluye con un balance del papel de la izquierda y de los retos que aún tiene por delante.

  11. Costo de flete de las exportaciones chilenas: 2000-2008

    Gonzalo Becerra M.; Claudio Vicuña U.

    2008-01-01

    El costo de flete que enfrentan las exportaciones chilenas muestra una trayectoria creciente en el lapso 2000-2008, caracterizada por oscilaciones. Las cifras del primer semestre de 2008, medidas en términos reales, se acercan a los US$75/tonelada, mientras que en 2000 se ubicaban en torno a los US$50/tonelada. A partir de la influencia que ejerce el cobre, el sector más relevante de las exportaciones chilenas lo constituye el mineral, con un costo de flete que se ha más que duplicado en el p...

  12. Somatic embryogenesis of Carica Papaya

    This paper describes the somatic embryogenesis of Carica papaya. Culture medium used was1/2 strength MS basal medium supplemented with 6% sucrose, 0.27 % agar, glutamine and various concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D). After 8 weeks in culture, the best concentration of 2,4-D to induce somatic embryo is at 45.2 μM. (Author)

  13. Gene Technology for Papaya Ringspot Virus Disease Management

    Md. Abul Kalam Azad; Latifah Amin; Nik Marzuki Sidik

    2014-01-01

    Papaya (Carica papaya) is severely damaged by the papaya ringspot virus (PRSV). This review focuses on the development of PRSV resistant transgenic papaya through gene technology. The genetic diversity of PRSV depends upon geographical distribution and the influence of PRSV disease management on a sequence of PRSV isolates. The concept of pathogen-derived resistance has been employed for the development of transgenic papaya, using a coat protein-mediated, RNA-silencing mechanism and replicase...

  14. Ocorrência de Ovulariopsis papayae Bijl em folhas de mamoeiro Occurrence of Ovulariopsis papayae Bijl in papaya leaves

    Ivan José Antunes Ribeiro

    1988-01-01

    Full Text Available É citada pela primeira vez, em nossas condições, a ocorrência de oídio causado por Ovulariopsis papayae Bijl em folhas de mamoeiro (Cacica papaya L.. São descritas a sintomatologia, as características morfológicas do patógeno e o teste de patogenicidade.The occurrence causing powdery mildew in papaya leaves was observed for the first time in the State of São Paulo, Brazil. The symptomatology and the morphological characteristics of the pathogen were described and the pathogenicity was comproved.

  15. The draft genome of the transgenic tropical fruit tree papaya ( Carica papaya Linnaeus)

    Ming, Ray; Hou, Shaobin; Feng, Yun; Yu, Qinguyi; Thimmapuram, Jyothi

    2008-01-01

    Papaya, a fruit crop cultivated in tropical and subtropical regions, is known for its nutritional benefits and medicinal applications. Here we report a 3 draft genome sequence of 'SunUp' papaya, the first commercial virus-resistant transgenic fruit tree1 to be sequenced. The papaya genome is three times the size of the Arabidopsis genome, but contains fewer genes, including significantly fewer disease-resistance gene analogues. Comparison of the five sequenced genomes suggests a minimal angio...

  16. The draft genome of the transgenic tropical fruit tree papaya (Carica papaya Linnaeus)

    Ming, Ray; Hou, Shaobin; Feng, Yun; Yu, Qingyi; Dionne-Laporte, Alexandre; Saw, Jimmy H.; Senin, Pavel; Wang, Wei; Ly, Benjamin V; Lewis, Kanako L. T.; Salzberg, Steven L.; Feng, Lu; Jones, Meghan R; Skelton, Rachel L; Murray, Jan E

    2008-01-01

    Papaya, a fruit crop cultivated in tropical and subtropical regions, is known for its nutritional benefits and medicinal applications. Here we report a 3× draft genome sequence of ‘SunUp’ papaya, the first commercial virus-resistant transgenic fruit tree1 to be sequenced. The papaya genome is three times the size of the Arabidopsis genome, but contains fewer genes, including significantly fewer disease-resistance gene analogues. Comparison of the five sequenced genomes suggests a minimal angi...

  17. Effect of Biopreservatives on Storage Life of Papaya (Carica papaya L.)

    Fatema H. Brishti; Jawadul Misir; Ayesha Sarker

    2013-01-01

    In this experiment the effect on post-harvest preservation of papaya (Carica papaya L.) fruit coated with either Aloe gel (AG; 100%) or papaya leaf extract with Aloe gel (PLEAG; 1:1) was studied. To evaluate the role of coating on ripening behavior and quality of papaya the uncoated and coated fruits were stored and ripened at room temperature (25 °C-29 °C) and 82-84% relative humidity. Physico-chemical properties were analyzed at 4 day intervals during the storage period. The incidence of ...

  18. Improving Tenderness of Spent Layer Hens Meat Using Papaya Leaves (Carica papaya

    H. O. Abdalla, N. N. A. Ali, F. S. Siddig and S. A. M. Ali*

    2013-01-01

    Full Text Available Two experiments were performed to study the use of papaya leaves as a meat tenderizer. The first experiment was to evaluate the effect of papaya dry leaves added to hen’s diet before slaughter. Spent hens (n=48 were used, half of them were fed a concentrate ration containing10% dried papaya leaves powder (DPLP while others received layer ration (Control, for 10 days. The second experiment involved a comparison between papaya leaves juice (PLJ, fresh papaya leaves (FPL and vinegar solution (VS as marinades applied to meat for one or two hours before cooking. Spent layer hens (n=42 were used for tenderness evaluation method. After slaughtering and preparing the chickens two methods of cooking were used (oven and moist cooking. The cooked parts (breast, thigh and drumstick were subjected to a panel test evaluation according to a designed questionnaire. Addition of dried papaya leaves powder to spent layer hens ration significantly (P≤0.05 increased the level of meat tenderness. Moist cooking had significantly (P≤0.05 improved meat tenderization compared to oven cooking. Meat treated with fresh papaya leaves had significantly (P≤0.05 improved tenderness. It was concluded that wrapping the tough meat of spent layer hens with fresh papaya leaves for one hour and moist cooking improve tenderness of meat.

  19. Molecular detection of Papaya meleira virus in the latex of Carica papaya by RT-PCR

    Araujo, de M.M.M.; Tavares, E.T.; Silva, da F.R.; Marinho, V.L.D.; Souza, M.T.

    2007-01-01

    A RT-PCR assay was developed for early and accurate detection of Papaya meleira virus (PMeV) in the latex from infected papayas. The meleira disease is characterized by an excessive exudation of more fluidic latex from fruits, leaves and stems. This latex oxidises and gives the fruit a ¿sticky¿ text

  20. The draft genome of the transgenic tropical fruit tree papaya (Carica papaya Linnaeus).

    We report a draft genome sequence of ‘SunUp’ papaya, the first commercial virus-resistant transgenic fruit tree crop. The papaya genome has more than two times the DNA but about 28% fewer genes than the genome of the related botanical model Arabidopsis. A lack of genome duplication, atypical of angi...

  1. Validation and use of DNA markers for sex determination in papaya (Carica papaya)

    Profitable papaya production requires female and hermaphrodite plants in higher number than male plants. This is only possible if sex of plants is determined at an early growth stage. The present study was conducted to validate sex-linked DNA markers using plants from two Pakistani papaya varieties and subsequently utilize them for determination of sex in juvenile papaya plants. One hundred and five plants (including 49 male and 56 female) of two Pakistani Papaya varieties at flowering stage were screened with six DNA markers viz., W-11, T12, SDP, Napf-76Napf-76, PKBT4 and PKBT5. All male plants exhibited amplification of sex-linked alleles with markers T12 and W11, whereas, 96% and 95% of female plants showed the absence of sex-linked allele with these markers, respectively. Markers SDP, PKBT5 and Napf-76 showed the presence of sex-linked alleles in 98%, 96% and 93% of male plants, respectively, whereas the same markers showed the absence of sex-linked alleles in 100%, 96% and 94% of female plants. One marker, PKBT4 could not produce expected PCR amplification reported previously. The five DNA markers were further used to screen 171 papaya seedlings. These markers clearly differentiated male and female sex types in the studied papaya plants. Results of our study are likely to facilitate Pakistani papaya breeders and growers to incorporate DNA based screening at juvenile stage to determine sex at early stage and to ensure profitable papaya production. (author)

  2. Papaya (Carica papaya) lysozyme is a member of the family 19 (Basic, class II) chitinases

    Subroto, T; Sufiati, S; Beintema, JJ

    1999-01-01

    The most comprehensive studies on a plant lysozyme (EC 3.2.1.17) are those on the enzyme from papaya (Carica papaya) latex, published in 1967 and 1969. However, the N-terminal amino acid sequence of five amino acid sequence of this enzyme, determined by manual Edman degradation, did not allow assign

  3. Erwinia mallotivora sp., a New Pathogen of Papaya (Carica papaya in Peninsular Malaysia

    Noriha Mat Amin

    2010-12-01

    Full Text Available Erwinia mallotivora was isolated from papaya infected with dieback disease showing the typical symptoms of greasy, water-soaked lesions and spots on leaves. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain belonged to the genus Erwinia and was united in a monophyletic group with E. mallotivora DSM 4565 (AJ233414. Earlier studies had indicated that the causal agent for this disease was E. papayae. However, our current studies, through Koch’s postulate, have confirmed that papaya dieback disease is caused by E. mallotivora. To our knowledge, this is the first new discovery of E. mallotivora as a causal agent of papaya dieback disease in Peninsular Malaysia. Previous reports have suggested that E. mallotivora causes leaf spot in Mallotus japonicus. However, this research confirms it also to be pathogenic to Carica papaya.

  4. Erwinia mallotivora sp., a new pathogen of papaya (Carica papaya) in Peninsular Malaysia.

    Amin, Noriha Mat; Bunawan, Hamidun; Redzuan, Rohaiza Ahmad; Jaganath, Indu Bala S

    2010-01-01

    Erwinia mallotivora was isolated from papaya infected with dieback disease showing the typical symptoms of greasy, water-soaked lesions and spots on leaves. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain belonged to the genus Erwinia and was united in a monophyletic group with E. mallotivora DSM 4565 (AJ233414). Earlier studies had indicated that the causal agent for this disease was E. papayae. However, our current studies, through Koch's postulate, have confirmed that papaya dieback disease is caused by E. mallotivora. To our knowledge, this is the first new discovery of E. mallotivora as a causal agent of papaya dieback disease in Peninsular Malaysia. Previous reports have suggested that E. mallotivora causes leaf spot in Mallotus japonicus. However, this research confirms it also to be pathogenic to Carica papaya. PMID:21339975

  5. CARICA PAPAYA: A COMPLETE PACKAGE OF NUTRITIONALAND MEDICINAL BENEFITS

    Karabhari Rekha Bhaskar

    2014-01-01

    Papaya (Carica papaya Linn.) is well known for its exceptional nutritional and medicinal properties throughout the world. From the times immemorial, the whole Carica papaya plant including its leaves, seeds, ripe and unripe fruits and their juice is used as traditional medicine. The fruit has a large oval shape, yellowish -green skin and yellow flesh. Now a day, Carica papaya is considered as nutraceutical fruit due to its multi faceted medicinal and nutritional properties. Th...

  6. Diversity of Papaya ringspot virus isolates in Puerto Rico

    Papaya ringspot virus (PRSV) devastates papaya production worldwide. In Puerto Rico, papaya fields can be completely infected with PRSV within a year of planting. Information about the diversity of the Puerto Rican PRSV population is relevant in order to establish a control strategy in the island. T...

  7. Transgenic Papaya: Can We Proceed Beyond the Hawaiian Experience?

    The story of the development, deregulation, and commercialization of the papaya ringspot virus (PRSV) resistant transgenic SunUp and Rainbow papaya for Hawaii is quite well known at least among plant virologist and knowledgeable people in the field of papaya. Thus, the story will be only briefly r...

  8. Correlation between ethylene emission and skin colour changes during papaya (Carica papaya L.) fruit ripening

    da Silva, M. G.; Oliveira, J. G.; Vitoria, A. P.; Corrêa, S. F.; Pereira, M. G.; Campostrini, E.; Santos, E. O.; Cavalli, A.; Vargas, H.

    2005-06-01

    The skin colour changes and ethylene emission rates were monitored during papaya (C. papaya L.) fruit ripening. Two groups of papaya (‘Formosa’ and ‘Solo’) were applied in this study. The total colour difference was used as measured parameter and the corresponding half time of its saturation was used as correlation parameter. A high correlation factor between the saturation half time and corresponding climacteric peak time was found. It was concluded that high ethylene emission rate in ‘Solo’ fruit promotes a quick change of the total colour difference.

  9. Effect of gamma irradiation in papaya (Carica papaya L.) harvested in three degrees of maturation

    The papaya is a fragile tropical fruit with thin skin, susceptible to post harvest diseases and mechanical injuries. Furthermore, it is sensible to low temperatures and at normal conditions it ripens rapidly, increasing the difficulties with storage. The objective of this work was to evaluate the influence of gamma irradiation in papayas harvested in three degrees of maturation, in order to increase shelf life. To accomplish that, papayas were harvested in perfect quality conditions, washed, submitted to carnauba wax and selected by skin coloration into three distinct degrees of maturation: Mat 0, or beginning of yellow coloration; Mat 1, yellow stripes more developed, and Mat 2, one third yellow. Half of them were submitted to irradiation with 0, 75 kGy, while the others became control treatment. They were analyzed in four different periods of conservation, which were 1 day after irradiation (DAI) refrigerated (11 +- 1 deg C), 14 DAI refrigerated, 14 DAI refrigerated + 3 at room temperature (RT = 24 deg +- 2 deg C) and 14 DAI refrigerated + 6 at RT. The effect of irradiation was not influenced by the maturation degree at harvest. Irradiation promoted firmness maintenance of papaya and, therefore, delayed ripening; modified the green color of papaya to a lighter tone, which determined more homogeneity in the skin yellow color development (greater values of L* and b*), and turned the papaya flesh color lighter (rower values of b*). There was no effect of irradiation in papaya weight loss, in the occurrence of diseases, in the development of surface yellow color, in the parameter a* of papaya skin color, in the parameters L*, a* and chroma of flesh color, p H and total soluble solids content. Visual and organoleptic sensorial tests were accomplished with papayas from a new delivery in the conservation period of 14 DAI refrigerated + 3 at RT. In the visual test was evaluated the appearance of papaya in the following treatments: Mat O control, Mat O irradiated, Mat 1

  10. Papaya Development Model As A Competitive Local Superior Commodity

    Reny Sukmawani

    2014-12-01

    Full Text Available The aim of this research is to study the comparative advantage and papaya competitive and to design its development model by using the approach of local base agriculture development. This research uses survey method. The resulting research shows that papaya is a base commodity that has comparative advantage and competitive. The development papaya in the district of Sukabumi is quite good bases on eight superior creations. But in order to be the main sector in economic development and has a competition, the development of papaya must concern to its influence factors. In supporting papaya development as a competitive local superior commodity, it needs to be done some efforts are as follows: (1 increase a skillful worker; (2 improve business management; (3 increase papaya productivity by using technology and study papaya planted technology in specific local superior commodity; (4 develop the involvement of the business relation; (5 provide market information and information technology network; and (6 improve infrastructures.

  11. Free radical scavenging activity of papaya juice

    Papaya juice is an efficient scavenger of highly reactive hydroxyl radicals (OH radical) formed during 60Co irradiation of water. The OH anion radicals were detected by the electron spin resonance (ESR) technique of spin trapping using DMPO (5,5-dimethyl-1-pyrroline-N-oxide) or by a colorimetric assay in which salicylate is converted into polyhydroxybenzoic acids. Papaya juice is also able to quench the ESR signal of a stable free radical (TEMPOL) and the ESR signal of the DMPO-OH adduct. The active substance(s) in papaya juice are heat-stable, dialyzable, and soluble in water but not in lipid solvents. The active agents do not appear to be ascorbate, tocopherol, or carotenoids. (author)

  12. Uptake of 32P labelled superphosphate by endomycorrhizal papaya (Carica papaya cv. Coorg honey dew)

    In papaya (Carica papaya cv. Coorg honey dew), there was an increase in 32P uptake and total phosphorus in plants inoculated with mycorrhizal fungi Glomus mossae and G. fasciculatum. Phosphorus derived from fertilizer (Pdff) was lower in mycorrhizal plants while soil derived P, utilisation of P and A values were higher showing thereby that mycorrhizal plants had utilised forms of phosphorus not available to non-mycorrhizal plants. (author). 13 refs., 1 tab

  13. Gene Technology for Papaya Ringspot Virus Disease Management

    Md. Abul Kalam Azad

    2014-01-01

    Full Text Available Papaya (Carica papaya is severely damaged by the papaya ringspot virus (PRSV. This review focuses on the development of PRSV resistant transgenic papaya through gene technology. The genetic diversity of PRSV depends upon geographical distribution and the influence of PRSV disease management on a sequence of PRSV isolates. The concept of pathogen-derived resistance has been employed for the development of transgenic papaya, using a coat protein-mediated, RNA-silencing mechanism and replicase gene-mediated transformation for effective PRSV disease management. The development of PRSV-resistant papaya via post-transcriptional gene silencing is a promising technology for PRSV disease management. PRSV-resistant transgenic papaya is environmentally safe and has no harmful effects on human health. Recent studies have revealed that the success of adoption of transgenic papaya depends upon the application, it being a commercially viable product, bio-safety regulatory issues, trade regulations, and the wider social acceptance of the technology. This review discusses the genome and the genetic diversity of PRSV, host range determinants, molecular diagnosis, disease management strategies, the development of transgenic papaya, environmental issues, issues in the adoption of transgenic papaya, and future directions for research.

  14. Gene technology for papaya ringspot virus disease management.

    Azad, Md Abul Kalam; Amin, Latifah; Sidik, Nik Marzuki

    2014-01-01

    Papaya (Carica papaya) is severely damaged by the papaya ringspot virus (PRSV). This review focuses on the development of PRSV resistant transgenic papaya through gene technology. The genetic diversity of PRSV depends upon geographical distribution and the influence of PRSV disease management on a sequence of PRSV isolates. The concept of pathogen-derived resistance has been employed for the development of transgenic papaya, using a coat protein-mediated, RNA-silencing mechanism and replicase gene-mediated transformation for effective PRSV disease management. The development of PRSV-resistant papaya via post-transcriptional gene silencing is a promising technology for PRSV disease management. PRSV-resistant transgenic papaya is environmentally safe and has no harmful effects on human health. Recent studies have revealed that the success of adoption of transgenic papaya depends upon the application, it being a commercially viable product, bio-safety regulatory issues, trade regulations, and the wider social acceptance of the technology. This review discusses the genome and the genetic diversity of PRSV, host range determinants, molecular diagnosis, disease management strategies, the development of transgenic papaya, environmental issues, issues in the adoption of transgenic papaya, and future directions for research. PMID:24757435

  15. Update on the development of virus-resistant papaya: Virus-resistant transgenic papaya for people in rural communities of Thailand

    Papaya (Carica papaya L.) is one of the most important and preferred crops in rural communities in Thailand. Papaya ringspot virus (PRSV) is a serious disease of papaya throughout Thailand. Efforts to control the virus by various methods either have not been successful or have not resulted in sustai...

  16. PROCESOS DE CANONIZACIÓN DE LA LITERATURA CHILENA

    Iván Carrasco M

    2008-11-01

    Full Text Available Este trabajo estudia los procesos de canonización en la literatura chilena y sus relaciones con las identidades socioculturales, desde la generación romántica de 1852 (ó 1842 hasta la última década del siglo XX, delimitando sus autores, textos y metatextos canónicos. La hipótesis que organiza el trabajo es que la literatura chilena se inició en torno a un canon único, que ha sido reemplazado a través del tiempo por cánones plurales, paralelos y contrapuestos.This paper studies the literary canonization processes in Chilean literature andtheir relations with social and cultural identities. Its purpose is to establish the boundaries of the texts and metatexts written by Chilean canonical authors, starting with the romantic generation of 1852 (or 1842 and ending up with the works published in the last decade of 'the XX century. The hypothesis of this essay is that Chilean literature can be said to have its origins focused on a single canon, which is later replaced by a series of plural, parallel and even opposite canons.

  17. The evolution of sex chromosomes in papaya

    Yu, Qingyi; Moore, Paul H.; Alam, Maqsudul; Jiang, Jiming; Paterson, Andrew H.; Vyskot, Boris; Ming, Ray

    San Diego, 2006. W340-W340. [Plant & Animal Genomes XIV Conference. 14.01.2006-18.01.2006, San Diego] R&D Projects: GA ČR(CZ) GA521/06/0056; GA MŠk(CZ) LC06004 Institutional research plan: CEZ:AV0Z50040507 Keywords : evolution * sex chromosomes * papaya Subject RIV: BO - Biophysics

  18. Transgenic Papaya: Development, Release, Impact, and Challenges

    Although the technology for developing virus-resistant transgenic plants through the use of the coat protein of a virus was unveiled twenty years ago, it is surprising to note that only a three virus-resistant plants (squash, potato, and papaya) have been commercialized in the U.S. The transgenic p...

  19. CARICA PAPAYA: A COMPLETE PACKAGE OF NUTRITIONALAND MEDICINAL BENEFITS

    Karabhari Rekha Bhaskar

    2014-07-01

    Full Text Available Papaya (Carica papaya Linn. is well known for its exceptional nutritional and medicinal properties throughout the world. From the times immemorial, the whole Carica papaya plant including its leaves, seeds, ripe and unripe fruits and their juice is used as traditional medicine. The fruit has a large oval shape, yellowish -green skin and yellow flesh. Now a day, Carica papaya is considered as nutraceutical fruit due to its multi faceted medicinal and nutritional properties. The prominent medicinal properties of Carica papaya include anti-fertility, uterotonic, and diuretic, anti-hypertensive, wound healing, anti bacterial activities. Nutritionally the whole plant contains enzymes, vitamin A, vitamin C, B-complex vitamins and potassium. The present article reviews the nutritional and medicinal uses of Carica papaya.

  20. Optimization of process parameters for osmotic dehydration of papaya cubes

    S.K. Jain; R. C. Verma; Murdia, L. K.; Jain, H. K.; Sharma, G. P.

    2010-01-01

    Process temperature (30, 40 and 50 °C), syrup concentration (50, 60 and 70o Brix) and process time (4, 5 and 6 h) for osmotic dehydration of papaya (Carica papaya) cubes were optimized for the maximum water loss and optimum sugar gain by using response surface methodology. The peeled and pre-processed papaya cubes of 1 cm size were immersed in sugar syrup at constant temperature water bath having syrup to papaya cubes ratio of 4:1 (w/w). The cubes were removed from bath at pre-decided time, r...

  1. Physical Characteristics of the Leaves and Latex of Papaya Plants Infected with the Papaya meleira Virus

    Anuar Magaña-Álvarez

    2016-04-01

    Full Text Available Sticky disease, which is caused by Papaya meleira virus (PMeV, is a significant papaya disease in Brazil and Mexico, where it has caused severe economic losses, and it seems to have spread to Central and South America. Studies assessing the pathogen-host interaction at the nano-histological level are needed to better understand the mechanisms that underlie natural resistance. In this study, the topography and mechanical properties of the leaf midribs and latex of healthy and PMeV-infected papaya plants were observed by atomic force microscopy and scanning electron microscopy. Healthy plants displayed a smooth surface with practically no roughness of the leaf midribs and the latex and a higher adhesion force than infected plants. PMeV promotes changes in the leaf midribs and latex, making them more fragile and susceptible to breakage. These changes, which are associated with increased water uptake and internal pressure in laticifers, causes cell disruption that leads to spontaneous exudation of the latex and facilitates the spread of PMeV to other laticifers. These results provide new insights into the papaya-PMeV interaction that could be helpful for controlling papaya sticky disease.

  2. Physical Characteristics of the Leaves and Latex of Papaya Plants Infected with the Papaya meleira Virus

    Magaña-Álvarez, Anuar; Vencioneck Dutra, Jean Carlos; Carneiro, Tarcio; Pérez-Brito, Daisy; Tapia-Tussell, Raúl; Ventura, Jose Aires; Higuera-Ciapara, Inocencio; Fernandes, Patricia Machado Bueno; Fernandes, Antonio Alberto Ribeiro

    2016-01-01

    Sticky disease, which is caused by Papaya meleira virus (PMeV), is a significant papaya disease in Brazil and Mexico, where it has caused severe economic losses, and it seems to have spread to Central and South America. Studies assessing the pathogen-host interaction at the nano-histological level are needed to better understand the mechanisms that underlie natural resistance. In this study, the topography and mechanical properties of the leaf midribs and latex of healthy and PMeV-infected papaya plants were observed by atomic force microscopy and scanning electron microscopy. Healthy plants displayed a smooth surface with practically no roughness of the leaf midribs and the latex and a higher adhesion force than infected plants. PMeV promotes changes in the leaf midribs and latex, making them more fragile and susceptible to breakage. These changes, which are associated with increased water uptake and internal pressure in laticifers, causes cell disruption that leads to spontaneous exudation of the latex and facilitates the spread of PMeV to other laticifers. These results provide new insights into the papaya-PMeV interaction that could be helpful for controlling papaya sticky disease. PMID:27092495

  3. Physical Characteristics of the Leaves and Latex of Papaya Plants Infected with the Papaya meleira Virus.

    Magaña-Álvarez, Anuar; Vencioneck Dutra, Jean Carlos; Carneiro, Tarcio; Pérez-Brito, Daisy; Tapia-Tussell, Raúl; Ventura, Jose Aires; Higuera-Ciapara, Inocencio; Fernandes, Patricia Machado Bueno; Fernandes, Antonio Alberto Ribeiro

    2016-01-01

    Sticky disease, which is caused by Papaya meleira virus (PMeV), is a significant papaya disease in Brazil and Mexico, where it has caused severe economic losses, and it seems to have spread to Central and South America. Studies assessing the pathogen-host interaction at the nano-histological level are needed to better understand the mechanisms that underlie natural resistance. In this study, the topography and mechanical properties of the leaf midribs and latex of healthy and PMeV-infected papaya plants were observed by atomic force microscopy and scanning electron microscopy. Healthy plants displayed a smooth surface with practically no roughness of the leaf midribs and the latex and a higher adhesion force than infected plants. PMeV promotes changes in the leaf midribs and latex, making them more fragile and susceptible to breakage. These changes, which are associated with increased water uptake and internal pressure in laticifers, causes cell disruption that leads to spontaneous exudation of the latex and facilitates the spread of PMeV to other laticifers. These results provide new insights into the papaya-PMeV interaction that could be helpful for controlling papaya sticky disease. PMID:27092495

  4. Proteomic analysis of papaya (Carica papaya L.) displaying typical sticky disease symptoms.

    Rodrigues, Silas P; Ventura, José A; Aguilar, Clemente; Nakayasu, Ernesto S; Almeida, Igor C; Fernandes, Patricia M B; Zingali, Russolina B

    2011-07-01

    Papaya (Carica papaya L.) hosts the only described laticifer-infecting virus (Papaya meleira virus, PMeV), which is the causal agent of papaya sticky disease. To understand the systemic effects of PMeV in papaya, we conducted a comprehensive proteomic analysis of leaf samples from healthy and diseased plants grown under field conditions. First, a reference 2-DE map was established for proteins from healthy samples. A total of 486 reproducible spots were identified, and MALDI-TOF-MS/MS data identified 275 proteins accounting for 159 distinct proteins from 231 spots that were annotated. Second, the differential expression of proteins from healthy and diseased leaves was determined through parallel experiments, using 2-DE and DIGE followed by MALDI-TOF-MS/MS and LC-IonTrap-MS/MS, respectively. Conventional 2-DE analysis revealed 75 differentially expressed proteins. Of those, 48 proteins were identified, with 26 being upregulated (U) and 22 downregulated (D). In general, metabolism-related proteins were downregulated, and stress-responsive proteins were upregulated. This expression pattern was corroborated by the results of the DIGE analysis, which identified 79 differentially expressed proteins, with 23 identified (17 U and 6 D). Calreticulin and the proteasome subunits 20S and RPT5a were shown to be upregulated during infection by both 2-DE and DIGE analyses. These data may help shed light on plant responses against stresses and viral infections. PMID:21630455

  5. COMPARATIVE STUDIES ON ANTHELMINTIC POTENTIAL OF CUCURBITA MAXIMA (PUMPKIN SEEDS AND CARICA PAPAYA (PAPAYA SEEDS

    Sengupta Rupa

    2013-08-01

    Full Text Available The crude extract of Carica papaya (papaya seeds (CP and Cucurbita maxima (Pumpkin seeds (CM were assayed against adult earthworms (Pheretima posthuma for the evaluation of anthelmintic activity. Various concentrations of both extracts were tested and results were expressed in terms of time for paralysis (P and time for death (D of worms. Albendazole was used as a reference standard. The result showed that in both of the extracts (i.e. CP and CM dose of 60 mg / ml possesses more wormicidal activity. The time of paralysis was 1.88 ± 0.52 minute and 1.93 ± 0.57 minute whereas the time of death was 3.45 ± 0.17 minute and 4.90 ± 0.18 minute in the case of Carica papaya and Cucurbita maxima respectively. In conclusion, the use of seeds of Carica papaya (CP and Cucurbita maxima (CM for anthelmintic activity have been confirmed and further studies are suggested to isolate the active principles responsible for the activity. Both the extracts showed significant anthelmintic activity, but the comparative study showed that out of these two, Carica papaya proves to be a better anthelmintic remedy.

  6. Digital transcriptome analysis of putative sex-determination genes in papaya (Carica papaya.

    Naoya Urasaki

    Full Text Available Papaya (Carica papaya is a trioecious plant species that has male, female and hermaphrodite flowers on different plants. The primitive sex chromosomes genetically determine the sex of the papaya. Although draft sequences of the papaya genome are already available, the genes for sex determination have not been identified, likely due to the complicated structure of its sex-chromosome sequences. To identify the candidate genes for sex determination, we conducted a transcriptome analysis of flower samples from male, female and hermaphrodite plants using high-throughput SuperSAGE for digital gene expression analysis. Among the short sequence tags obtained from the transcripts, 312 unique tags were specifically mapped to the primitive sex chromosome (X or Y(h sequences. An annotation analysis revealed that retroelements are the most abundant sequences observed in the genes corresponding to these tags. The majority of tags on the sex chromosomes were located on the X chromosome, and only 30 tags were commonly mapped to both the X and Y(h chromosome, implying a loss of many genes on the Y(h chromosome. Nevertheless, candidate Y(h chromosome-specific female determination genes, including a MADS-box gene, were identified. Information on these sex chromosome-specific expressed genes will help elucidating sex determination in the papaya.

  7. Influence of ripening stages on antioxidant properties of papaya fruit (Carica papaya L.)

    Addai, Zuhair Radhi; Abdullah, Aminah; Mutalib, Sahilah Abd.

    2013-11-01

    Papaya (Carica papaya L. cv Eksotika) is one of the most commonly consumed tropical fruits by humans, especially Malaysians. The objective of this study was to determine the phenolic compounds and antioxidants activity in different ripening stages of papaya fruit. The fruits were harvested at five different, stages RS1, RS2, RS3, RS4, and RS5 corresponding to 12, 14, 16, 18, and 20 weeks after anthesis, respectively. Papayas fruit at five different stage of ripening were obtained from farms at Pusat Flora Cheras, JabatanPertanian and Hulu Langat Semenyih, Selangor, Malaysia. The antioxidants activity were analyzed using the total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant Power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). The analyses were conducted in triplicate and the data were subjected to statistical analysis using SPSS. The results showed significant differences (P< 0.05) were found at different stages of ripening. The total phenol content TPC, TFC, FRAP and DPPH values increased significantly (P<0.05) with the ripening process. The results showed the important role of the ripening stage in increasing the antioxidant content of papaya fruits.

  8. Fusarium species associated with fruit rot of banana (Musa spp.), papaya (Carica papaya) and guava (Psidium guajava)

    Zakaria, L.; Mazzura, W. C.; Kong, W. H.; S. Baharuddin

    2012-01-01

    A total of 60 isolates of Fusarium were isolated from fruit rot of banana (Musa spp.), papaya (Carica papaya) and guava(Psidium guajava). The most common species recovered from the fruit rot of the three fruit crops were F. semitectum(40 %), F. solani (38.3 %), F. verticillioides (11.7 %) and F. oxysporum (10 %). Fusarium semitectum was isolated from fruit rot of banana, papaya and guava; F. oxysporum from banana and papaya; F. solani from banana and guava and F.verticillioides from banana. F...

  9. Extraction and characteristics of seed oil from Papaya (Carica papaya in Congo-Brazzaville

    G. Bouanga-Kalou

    2011-03-01

    Full Text Available Papaya seeds were collected and dried. This study was carried out on papaya seed to clarify their proximate composition and the characteristics of the extracted oil including unsaponifiable matter and fatty acid composition. The seed is a rich source of protein (26.78% and crude fiber (21.4%. Palmitic acid was the main saturated fatty acid (15.22%, while linoleic acid was the major unsaturated fatty acid (76.38% in all lipid classes. The physical properties of the oil extracts showed the state to be liquid at room temperature. Carica papaya seeds have ash content of 3.2% (with the presence of following minerals: K, Na, Ca, P and Mg. However, Ca and P occur in appreciable quantities (1821±2.12 mg/100 g dry matter and 1156±1.8 mg/100 g dry mater, respectively.

  10. Whole-photosynthesis and transpiration in field-grown papaya plants

    Papaya (Carica papaya L.) is a principal horticultural crop of tropical and subtropical regions. Knowledge of papaya response to environmental factors provides a scientific basis to develop management strategies to optimize fruit yield and quality. In papaya, the photosynthetic capacity also influ...

  11. Apertura y Eficiencia Productiva: La Industria Chilena, 1975-1988 Apertura y Eficiencia Productiva: La Industria Chilena, 1975-1988

    Enrique Román

    1993-03-01

    Full Text Available Apertura y Eficiencia Productiva: La Industria Chilena, 1975-1988 The results of Chile's profound economic liberalizations remain the subject of ongoing debate. Using the Total Factor Productivity approach (TFP; we review the industrial sector performance during the 1975-1988 period. Our analysis shows that greater competitiveness dictated by economic liberalization was not accomplished through technical change but, rather, through intensive factor use (1976-81, or extensive factor use (1984-88. After exhaustion of the "easy phase", new levels of productive efficiency necessitated technical innovation, that is, investment in equipment, training. and reorganization of productive processes. However, after the 1982-83 crisis, firms did not follow this path: previous financial stress and low wage costs determined a growth path based on extensive use of labor, thereby delaying technological modernization of the industrial sector.

  12. Extraction and characteristics of seed oil from Papaya (Carica papaya) in Congo-Brazzaville

    G. Bouanga-Kalou; A. Kimbonguila; J.M. Nzikou; F.B. Ganongo-Po; F.E. Moutoula; E. Panyoo-Akdowa; Th. Silou and S. Desobry

    2011-01-01

    Papaya seeds were collected and dried. This study was carried out on papaya seed to clarify their proximate composition and the characteristics of the extracted oil including unsaponifiable matter and fatty acid composition. The seed is a rich source of protein (26.78%) and crude fiber (21.4%). Palmitic acid was the main saturated fatty acid (15.22%), while linoleic acid was the major unsaturated fatty acid (76.38%) in all lipid classes. The physical properties of the oil extracts showed the ...

  13. Influence of gamma radiation on carbohydrates metabolism of ripening papaya (Carica papaya L. cv. Solo)

    Food irradiation is one of the most promising treatments that can be utilized for fruits disinfestation and extension of shelf life. The authors studied the influence of 0,5 kGy of Gamma irradiation on the soluble carbohydrates composition of papaya (Carica papaya L. cv. Solo) fruit, and on sucrose metabolizing enzymes: sucrose synthase (SS), sucrose-phosphate synthase, acid and neutral invertases activities, during ripening. The results demonstrated that ethylene production, total soluble sugars, sucrose content, and sucrose-phosphate synthase and invertases activities were affected by irradiation, but not respiration, glucose and fructose content, and SS activity. (author)

  14. Dwarf mutant of papaya (Carica papaya L.) induced by gamma rays

    Treating the seeds of papaya with 15 Krad of gamma rays, three plants were isolated from the M3 population. Repeated full sibmating among the progenies of these dwarf mutants helped in establishing a homozygous population of dwarf papaya in M6 generation. Though the per plant yield in mutant is less, nevertheless its importance is envisaged in the fact that it is likely to incur less damage by wind and storm, can be better managed and more number of plants can be accomodated per unit area, thus giving increased yield per hectare. (author)

  15. Carica papaya microRNAs are responsive to Papaya meleira virus infection.

    Paolla M V Abreu

    Full Text Available MicroRNAs are implicated in the response to biotic stresses. Papaya meleira virus (PMeV is the causal agent of sticky disease, a commercially important pathology in papaya for which there are currently no resistant varieties. PMeV has a number of unusual features, such as residence in the laticifers of infected plants, and the response of the papaya to PMeV infection is not well understood. The protein levels of 20S proteasome subunits increase during PMeV infection, suggesting that proteolysis could be an important aspect of the plant defense response mechanism. To date, 10,598 plant microRNAs have been identified in the Plant miRNAs Database, but only two, miR162 and miR403, are from papaya. In this study, known plant microRNA sequences were used to search for potential microRNAs in the papaya genome. A total of 462 microRNAs, representing 72 microRNA families, were identified. The expression of 11 microRNAs, whose targets are involved in 20S and 26S proteasomal degradation and in other stress response pathways, was compared by real-time PCR in healthy and infected papaya leaf tissue. We found that the expression of miRNAs involved in proteasomal degradation increased in response to very low levels of PMeV titre and decreased as the viral titre increased. In contrast, miRNAs implicated in the plant response to biotic stress decreased their expression at very low level of PMeV and increased at high PMeV levels. Corroborating with this results, analysed target genes for this miRNAs had their expression modulated in a dependent manner. This study represents a comprehensive identification of conserved miRNAs inpapaya. The data presented here might help to complement the available molecular and genomic tools for the study of papaya. The differential expression of some miRNAs and identifying their target genes will be helpful for understanding the regulation and interaction of PMeV and papaya.

  16. Carica papaya microRNAs are responsive to Papaya meleira virus infection.

    Abreu, Paolla M V; Gaspar, Clicia G; Buss, David S; Ventura, José A; Ferreira, Paulo C G; Fernandes, Patricia M B

    2014-01-01

    MicroRNAs are implicated in the response to biotic stresses. Papaya meleira virus (PMeV) is the causal agent of sticky disease, a commercially important pathology in papaya for which there are currently no resistant varieties. PMeV has a number of unusual features, such as residence in the laticifers of infected plants, and the response of the papaya to PMeV infection is not well understood. The protein levels of 20S proteasome subunits increase during PMeV infection, suggesting that proteolysis could be an important aspect of the plant defense response mechanism. To date, 10,598 plant microRNAs have been identified in the Plant miRNAs Database, but only two, miR162 and miR403, are from papaya. In this study, known plant microRNA sequences were used to search for potential microRNAs in the papaya genome. A total of 462 microRNAs, representing 72 microRNA families, were identified. The expression of 11 microRNAs, whose targets are involved in 20S and 26S proteasomal degradation and in other stress response pathways, was compared by real-time PCR in healthy and infected papaya leaf tissue. We found that the expression of miRNAs involved in proteasomal degradation increased in response to very low levels of PMeV titre and decreased as the viral titre increased. In contrast, miRNAs implicated in the plant response to biotic stress decreased their expression at very low level of PMeV and increased at high PMeV levels. Corroborating with this results, analysed target genes for this miRNAs had their expression modulated in a dependent manner. This study represents a comprehensive identification of conserved miRNAs inpapaya. The data presented here might help to complement the available molecular and genomic tools for the study of papaya. The differential expression of some miRNAs and identifying their target genes will be helpful for understanding the regulation and interaction of PMeV and papaya. PMID:25072834

  17. Physical Characteristics of the Leaves and Latex of Papaya Plants Infected with the Papaya meleira Virus

    Anuar Magaña-Álvarez; Jean Carlos Vencioneck Dutra; Tarcio Carneiro; Daisy Pérez-Brito; Raúl Tapia-Tussell; Jose Aires Ventura; Inocencio Higuera-Ciapara; Patricia Machado Bueno Fernandes; Antonio Alberto Ribeiro Fernandes

    2016-01-01

    Sticky disease, which is caused by Papaya meleira virus (PMeV), is a significant papaya disease in Brazil and Mexico, where it has caused severe economic losses, and it seems to have spread to Central and South America. Studies assessing the pathogen-host interaction at the nano-histological level are needed to better understand the mechanisms that underlie natural resistance. In this study, the topography and mechanical properties of the leaf midribs and latex of healthy and PMeV-infected pa...

  18. Erwinia mallotivora sp., a New Pathogen of Papaya (Carica papaya) in Peninsular Malaysia

    Noriha Mat Amin; Hamidun Bunawan; Rohaiza Ahmad Redzuan; Indu Bala S. Jaganath

    2010-01-01

    Erwinia mallotivora was isolated from papaya infected with dieback disease showing the typical symptoms of greasy, water-soaked lesions and spots on leaves. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain belonged to the genus Erwinia and was united in a monophyletic group with E. mallotivora DSM 4565 (AJ233414). Earlier studies had indicated that the causal agent for this disease was E. papayae. However, our current studies, through Koch’s postulate, have confirmed th...

  19. Penggunaan Sari Buah Pepaya ( Carica papaya L.) Dalam Sediaan Krim Pelembab

    Ferida, Teti

    2011-01-01

    Papaya (Carica Papaya L.) is one of sample natural material can be as moisturizing skin. Papaya contain some kind of enzyme, vitamin, and mineral. Papaya was rich of vitamin A, B1, and C. which very important to ward free radical. Beside vitamin, papaya also has mineral as calcium. Phosphor, potassium, iron, carbohidrat, protein and lipid. Containing of sugar in ripe papaya among sucrose, glucose and fructose. Based on the contains can be tried to do a research by papaya extract to made be...

  20. Gamma radiation in papaya harvested at three stages of maturation

    Papaya is a fragile, perishable fruit, highly accepted worldwide. To keep the quality of papaya from harvest to the consumers, conservation techniques are often used; among them is the application of gamma irradiation. The objective of this work was to evaluate gamma irradiation in papayas harvested at three degrees of maturation, in order to increase shelf life. Papayas were harvested in perfect quality conditions and selected by skin coloration into three distinct degrees of maturation: maturation 0, or beginning of yellow coloration; maturation 1, yellow stripes more developed, and maturation 2, one third yellow. Half of them were irradiated with 0.75 kGy, while the other half became control treatment. They were analyzed in four periods of conservation, which were 1 DAI (days after irradiation refrigerated at 11 ± 1 deg C), 14 DAI, 14 DAI + 3 DRT (room temperature at 24 deg C ± 2 deg C) and 14 DAI + 6 DRT. The papaya maturation degree at harvest did not influence the radiation effect. Irradiation maintained firmness of papaya and, therefore, delayed ripening; modified the green color of papaya to a lighter, more intense tone, which determined more homogeneity in the development of the skin's yellow color (greater values of L* and chroma). There was no effect of irradiation in papaya weight loss, occurrence of diseases, chroma of flesh color, p H and total soluble solids contents. (author)

  1. Papaya Ringspot Virus: Characteristics, Pathogenicity, Sequence Variability and Control

    Taxonomy: Papaya ringspot virus (PRSV) is an aphid-transmitted plant virus belonging to the genus Potyvirus of the family Potyviridae with a positive sense RNA genome. PRSV isolates belong to either one of two major strains, P-type or W-type. The P-type infects both papaya and cucurbits whereas th...

  2. Gamma radiation in papaya harvested at three stages of maturation

    Pimentel, Rodrigo Meirelles de Azevedo; Walder, Julio Marcos Melges [Centro de Energia Nuclear na Agricultura, Piracicaba, SP (Brazil). Lab. de Irradiacao de Alimentos]. E-mail: jmwalder@cena.usp.br

    2004-04-01

    Papaya is a fragile, perishable fruit, highly accepted worldwide. To keep the quality of papaya from harvest to the consumers, conservation techniques are often used; among them is the application of gamma irradiation. The objective of this work was to evaluate gamma irradiation in papayas harvested at three degrees of maturation, in order to increase shelf life. Papayas were harvested in perfect quality conditions and selected by skin coloration into three distinct degrees of maturation: maturation 0, or beginning of yellow coloration; maturation 1, yellow stripes more developed, and maturation 2, one third yellow. Half of them were irradiated with 0.75 kGy, while the other half became control treatment. They were analyzed in four periods of conservation, which were 1 DAI (days after irradiation refrigerated at 11 {+-} 1 deg C), 14 DAI, 14 DAI + 3 DRT (room temperature at 24 deg C {+-} 2 deg C) and 14 DAI + 6 DRT. The papaya maturation degree at harvest did not influence the radiation effect. Irradiation maintained firmness of papaya and, therefore, delayed ripening; modified the green color of papaya to a lighter, more intense tone, which determined more homogeneity in the development of the skin's yellow color (greater values of L{sup *} and chroma). There was no effect of irradiation in papaya weight loss, occurrence of diseases, chroma of flesh color, p H and total soluble solids contents. (author)

  3. 75 FR 22207 - Importation of Papayas From Colombia and Ecuador

    2010-04-28

    ... published in the Federal Register (74 FR 18161-18166, Docket No. APHIS-2008-0050) a proposal\\1\\ to amend the... Animal and Plant Health Inspection Service 7 CFR Part 319 RIN 0579-AC95 Importation of Papayas From... importation of commercial shipments of fresh papayas from Colombia and Ecuador into the continental...

  4. Effects of heat, irradiation and their combination on the keeping quality of papaya (carica papaya linn.)

    An experimental work has been done to determine the effect of gamma irradiation either alone or in combination with hot water dipping on the shelf life of fresh papaya. Mature green papayas of 'semangka paris' variety were treated under 3 different conditions, i.e. dipped in hot water at 50 Celsius degrees for 10 minutes; irradiated in air with a dose of 0.5 kGy; treated with combination of hot water dipping at 50 Celsius degrees for 10 minutes and irradiation with a dose of 0.5 kGy, and stored at ambient conditions. Quality evaluation was done subjectively during storage on the colour, texture, and taste. Besides, weight loss was also observed. Chemical characteristics, namely the contents of vitamin C, β-carotene, total soluble pectin, and reducing sugar were also determined as objective parameters. The results revealed that ripening of mature green papaya could be delayed by irradiation with the dose of 0.5 kGy. Best result was obtained from the combination treatment. The treated papayas were still acceptable up to 8 days of storage, whereas the untreated ones were acceptable only up to 5 days of storage. (author)

  5. Effect of Biopreservatives on Storage Life of Papaya (Carica papaya L.

    Fatema H. Brishti

    2013-04-01

    Full Text Available Normal 0 false false false EN-US X-NONE X-NONE In this experiment the effect on post-harvest preservation of papaya (Carica papaya L. fruit coated with either Aloe gel (AG; 100% or papaya leaf extract with Aloe gel (PLEAG; 1:1 was studied. To evaluate the role of coating on ripening behavior and quality of papaya the uncoated and coated fruits were stored and ripened at room temperature (25 °C-29 °C and 82-84% relative humidity. Physico-chemical properties were analyzed at 4 day intervals during the storage period. The incidence of disease attack was also visually observed. The overall results showed the superiority of AG and PLEAG coating in lengthening the shelf-life of papaya fruit compared to controls which showed significant decay from 6th day onward and complete decay within 12 days of storage. The AG and PLEAG coated fruits maintained their shelf life for 12 days and decayed at 16th day. The coated fruits also maintained their color, flavor and firmness up to 12 days of storage. An increase in ascorbic acid content (120.2 mg/100 g was also found in coated fruits in contrast to the control (59 mg/100 g. Only 27% disease incidence was observed in AG and 13% in PLEAG coated fruits as compared to control (100% during the storage period. The results of this study show that both AG and PLEAG coatings have excellent potential to be used on fresh produce to maintain quality and extend shelf-life.

  6. COMUNEROS ANDINOS EN LA ADMINISTRACIÓN CHILENA (ARICA, 1880-1929

    ALBERTO DÍAZ ARAYA

    2011-05-01

    Full Text Available Resumen Se evalúa el impacto generado en los sectores rurales andinos de Arica por la implementación de la estructura y burocracia administrativa chilena post Guerra del Pacífico. A través de registros documentales se hace explícita la participación de comuneros indígenas en la trama estatal chilena en un período de conflicto político y de identidades nacionales. Palabras claves: Comunidad andina – administración chilena – Arica y Tacna. Abstract Assesses the impact generated in the rural Andes Arica for implementing the administrative structure and bureaucracy in Chile after the Pacific War. Through documentary record makes explicit the participation of indigenous community members in the plot Chilean state over a period of political and national identities. Keywords : Andean Community - Chilean government - Arica and Tacna.

  7. Promover la salud mental con mujeres chilenas en desventaja social

    M Soledad Rivera

    2004-01-01

    Full Text Available Se descrive una parte de la experiencia del proyecto Chileno/Canadiense: “Promover la salud mental de las mujeres chilenas en desventaja social: Los Secretos de Maruja”. Se describen los objetivos, las etapas del proceso, de intervención y los resultados de las evaluaciones preliminares. Con la participación de las mujeres usuárias de los centros de salud, profesionales y autoridades de la comuna de La Pintana, se construyó, aplicó y evaluó un proyecto de intervención para apoyar a las mujeres en la toma de decisiones que favorezcan su salud mental personal, familiar y social. La intervención contempló el diseño, producción, distribución y evaluación de 12 folletos educativos tipo calendarios, cada uno respecto a tres temas de salud mental identificados por las mismas mujeres: Identidad y autoestima, Vínculo y Comunicación y Autocuidado. Además se involucró a las enfermeras que trabajan en los Centros de Salud, quienes incorporaron esta estrategia a la atención de salud habitual y evaluaron su impacto en la identidad, autonomía profesional y en la mejoría de la calidad del cuidado

  8. Development of Transgenic Papaya through Agrobacterium-Mediated Transformation

    Md. Abul Kalam Azad

    2013-01-01

    Full Text Available Transgenic papaya plants were regenerated from hypocotyls and immature zygotic embryo after cocultivation with Agrobacterium tumefaciens LBA-4404 carrying a binary plasmid vector system containing neomycin phosphotransferase (nptII gene as the selectable marker and β-glucuronidase (GUS as the reporter gene. The explants were co-cultivated with Agrobacterium tumefaciens on regeneration medium containing 500 mg/L carbenicillin + 200 mg/L cefotaxime for one week. The cocultivated explants were transferred into the final selection medium containing 500 mg/L carbenicillin + 200 mg/L cefotaxime + 50 mg/L kanamycin for callus induction as well as plant regeneration. The callus derived from the hypocotyls of Carica papaya cv. Shahi showed the highest positive GUS activities compared to Carica papaya cv. Ranchi. The transformed callus grew vigorously and formed embryos followed by transgenic plantlets successfully. The result of this study showed that the hypocotyls of C. papaya cv. Shahi and C. papaya cv. Ranchi are better explants for genetic transformation compared to immature embryos. The transformed C. papaya cv. Shahi also showed the maximum number of plant regeneration compared to that of C. papaya cv. Ranchi.

  9. Whole genome sequence analysis of unidentified genetically modified papaya for development of a specific detection method.

    Nakamura, Kosuke; Kondo, Kazunari; Akiyama, Hiroshi; Ishigaki, Takumi; Noguchi, Akio; Katsumata, Hiroshi; Takasaki, Kazuto; Futo, Satoshi; Sakata, Kozue; Fukuda, Nozomi; Mano, Junichi; Kitta, Kazumi; Tanaka, Hidenori; Akashi, Ryo; Nishimaki-Mogami, Tomoko

    2016-08-15

    Identification of transgenic sequences in an unknown genetically modified (GM) papaya (Carica papaya L.) by whole genome sequence analysis was demonstrated. Whole genome sequence data were generated for a GM-positive fresh papaya fruit commodity detected in monitoring using real-time polymerase chain reaction (PCR). The sequences obtained were mapped against an open database for papaya genome sequence. Transgenic construct- and event-specific sequences were identified as a GM papaya developed to resist infection from a Papaya ringspot virus. Based on the transgenic sequences, a specific real-time PCR detection method for GM papaya applicable to various food commodities was developed. Whole genome sequence analysis enabled identifying unknown transgenic construct- and event-specific sequences in GM papaya and development of a reliable method for detecting them in papaya food commodities. PMID:27006240

  10. Radiation-induced mutation breeding of papaya

    Irradiation-induced mutation breeding of papaya commenced at the Malaysian Agricultural Research and Development Institute (MARDI) in August, 2000. This research was initiated under a Coordinated Research Project (CRP - D23023) with assistance from the International Atomic Energy Agency (IAEA). In the preliminary dosimetry study, seeds from two local papaya varieties, Sekaki and Eksotika were irradiated, either as dry seeds or as pre-soaked seeds (soaked overnight in water and surface-dried) with radiation doses ranging from 0 to 300 Gy. 100 Gy dose was lethal for all wet presoaked seeds while dry seed did not show loss of viability, even at 300 Gy. From the growth data it was estimated that dose of 525 Gy reduced shoot elongation by 50%, and this dose was recommended for mass irradiation of dry seeds. For wet, pre-soaked seeds results indicated that 42.5 Gy was the optimal dose for mass irradiation. At this dose, both seeds germination and seedlings growth were reduced by 50%. In a massive irradiation experiment 2,000 Eksotika seeds were irradiated at 42.5 Gy (pre-soaked) and another 2,000 at 525 Gy (dry). In the M2 population, numerous physiological defects were observed, including stem splitting, leaf variegation and puckering, and crinkled dwarfs. In the M3 population, a wide variability was recorded for a number of traits. M3 seedlings derived from presoaked seeds irradiated a low 42.5 Gy dose presented a high number of plants that were shorter and more vigorous in leaf development compared to those irradiated at 525 Gy and to non-irradiated control seedlings. The distribution patterns of M3 progenies for nine quantitative field characters showed great variation, often exceeding the limits of the control population. There appears to be good prospects in improving Eksotika papaya especially in the development of dwarf trees with lower fruit bearing stature, higher total soluble solids in fruits and larger fruit size. Several M2 and M3 putative mutants also

  11. Pengujian Ekstrak n-Heksana dan Etanol terhadap Aktivitas Antibakteri Biji Pepaya (Carica papaya L.) dari Dua Varietas

    Ginting, Ovalina Sylvia

    2015-01-01

    Papaya seed can be beneficial as drug of digestion trouble, diarrhoea and skin disease.The aim research is understanding antibacterial activity from n-hexane extract and ethanol extract from seed of two varieties of papaya (Carica papaya L.) to Escherichia coli (ATCC 10536) and Staphylococcus aureus (ATCC 29737). Papaya seed used in this research came from seeds of bird papaya and chocolate papaya. Extraction was done of two type solvents which were polar (ethanol) and non polar (n-hexane)...

  12. New approach for papaya latex storage without virus degradation

    Silas P. Rodrigues; Andrade, Josemar S.; José A. Ventura; Fernandes, Patricia M. B.

    2009-01-01

    Papaya meleira virus (PMeV) is the causal agent of papaya (Carica papaya L.) sticky disease, which has been detected through analysis of its double-stranded RNA (dsRNA) genome from plant latex. In this work we demonstrate that PMeV dsRNA is protected during 25 days when latex is diluted in citrate buffer pH 5.0 (1:1 v/v) and maintained at -20ºC. At the same temperature, some protection was observed for pure latex or latex diluted in ultra-pure water. Conversely, the dsRNA was almost completel...

  13. Compositional changes during papaya fruit ripening

    The objective of this study was to investigate compositional changes during ripening of Baladi, Ekostika I and Ekostika II papaya fruit cultivars at 20±1°C and 85% -90% relative humidity. The fruits of the three cultivars exhibited a typical climacteric pattern of respiration with peak of respiration of 82,92 and 98 mg CO2/ kg-hr, reached after 10 days in the three cultivars, respectively. Weight loss, total soluble solids (TSS), total sugars and ascorbic acid content progressively increased during ripening of the three papaya cultivars. More increase in TSS and total sugars was observed after the climacteric peak of respiration. Fruit tissue firmness and total phenolic compounds decreased continuously during ripening in the three cultivars. Reducing sugars, total protein and titratable acidity steadily increased to reach a peak, which coincided with climacteric peak of respiration, and subsequently decreased afterwards. The local Baladi cultivar had a lower respiration rate, more firm and less weight loss during ripening, which may indicate a longer shelf life than the other two introduced cultivars. On the other hand, the introduced cultivars were higher in TSS, total and reducing sugars and ascorbic acid content and lower in titratable acidity and phenolic compounds, which may reflect a better eating quality.(Author)

  14. Radiation disinfestation as a quarantine treatment for solo papaya

    Solo papaya is subject to infestation by the Oriental fruit fly, Bactrocera dorsalis (Hendel). Since ethylene dibromide has been banned for use as a fumigant for fruits, gamma radiation was investigated as an alternative quarantine disinfestation treatment for solo papaya. Results of small-scale laboratory tests showed that 150 Gy completely prevented the emergence of B. dorsalis. For egg-infested solo papaya, pupal recovered decreased with increasing dose while insect survival was not associated with fruit maturity. For larvae-infested ones, insect survival, in general, was highest in the full-ripe stage and lowest in the one-fourth-ripe stage of the fruit. Confirmatory tests of the proposed treatment using more than 100,000 test insects revealed that 150 Gy radiation dose is sufficient to provide Probit 9 quarantine security for solo papaya. (author). 10 refs.; 1 fig.; 4 tabs

  15. Effects of chemical treatments on fresh-cut papaya.

    Albertini, Silvana; Lai Reyes, Andrés Enrique; Trigo, Juliana Moreno; Sarriés, Gabriel Adrián; Spoto, Marta Helena Fillet

    2016-01-01

    Four treatments (control, 0.1% cinnamaldehyde, 0.75% calcium chloride and combination of 0.1% cinnamaldehyde and 0.75% calcium chloride) were used to evaluate chemical effects on shelf life, quality and sensory acceptability of fresh-cut papaya (Carica papaya L.). Papaya slices were packed and covered with polypropylene film, stored at 5 °C; and evaluated after 1, 3, 6, 9, 12, and 15 days for microbiological and physicochemical changes. A sensory evaluation was performed at 1, 3, 6, 9, and 12 days. There was no occurrence of Salmonella, Escherichia coli or psychotropic bacteria. The cinnamaldehyde alone and a combination of cinnamaldehyde and calcium chloride treatments yielded better control of the total coliforms. The combination treatment decreased the CO2 concentration and increased the maintenance of papaya firmness. All the treatments had acceptability. The combination treatment was the most effective treatment for flavor, taste, and preservation until day 12. PMID:26213093

  16. Evaluation of fruit leather made from two cultivars of papaya

    ZUHAIR RADHI ADDAI

    2016-03-01

    Full Text Available Two papaya cultivars were used to manufacture fruit leather. The objective of this study was to formulate papaya leather from locally grown papaya using natural ingredients like pectin, honey and citric acid. The fresh fruits were pureed and mix with natural ingredients, and dried in an oven at 60°C for 12 hours. The physicochemical properties and antioxidant activity were determined.The results showed that fruit leather made from Hongkong cultivar is significantly (P<0.05 higher in sensory parameters as well as physicochemical properties and antioxidant activity. The phenolics content and antioxidant activity increased by process of drying the fruit leather compared to fresh fruits in both papaya cultivars. Therefore, the consumer requirements for healthy and safe food products were respected.

  17. Post-transcriptional gene silencing is involved in resistance of transgenic papayas to Papaya Ringspot Virus

    Ruanjan, P.; Kertbundit, Sunee; Juříček, Miloslav

    2007-01-01

    Roč. 51, č. 3 (2007), s. 517-520. ISSN 0006-3134 Grant ostatní: BIOTEC, NASDA(TH) BT-B-06-PG-14-4503 Institutional research plan: CEZ:AV0Z50380511 Source of funding: V - iné verejné zdroje Keywords : Carica papaya * reverse transcription PCR * COAT PROTEIN GENE Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.259, year: 2007

  18. KARAKTERISASI PAPAIN DARI DAUN PEPAYA (Carica Papaya L. CHARACTERIZATION OF PAPAIN FROM Carica Papaya L. LEAVES

    Zusfahair; Dian Riana Ningsih; Febrina Nur Habibah

    2014-01-01

    Enzim yang menempati urutan pertama dalam pemanfaatannya di bidang industri adalah protease. Protease dapat digunakan sebagai katalis untuk reaksi yang menggunakan pelarut organik. Penelitian ini bertujuan untuk mengetahui karakteristik ekstrak kasar papain dari daun pepaya (Carica papaya L.) yang meliputi suhu dan pH optimum, pengaruh EDTA dan ion-ion logam, serta kestabilannya dalam pelarut organik seperti metanol, aseton, dan toluena, serta potensinya sebagai katalis dalam pelarut organik....

  19. Optical and ultrastructural study of the pollen grain development in hermaphrodite papaya tree (Carica papaya L.)

    Lídia Márcia Silva Santos; Telma Nair Santana Pereira; Margarete Magalhães de Souza; Pedro Correa Damasceno Junior; Fabiane Rabelo da Costa; Beatriz Ferreira Ribeiro; Noil Gomes de Freitas; Messias Gonzaga Pereira

    2008-01-01

    The objective of this study was to describe the pollen grain development in hermaphrodite papaya tree. The flower buds were collected at different stages of the development and the anthers were treated chemically for observation under optical and electronic transmission microscopes. The pollen grain development followed the normal pattern described for the Angiosperms. The pollen grain development was described from meiocyte to the mature pollen grain. In the microsporogenesis, the microspore...

  20. ESR detection procedure of irradiated papaya containing high water content

    Kikuchi, Masahiro; Shimoyama, Yuhei; Ukai, Mitsuko; Kobayashi, Yasuhiko

    2011-05-01

    ESR signals were recorded from irradiated papaya at liquid nitrogen temperature (77 K), and freeze-dried irradiated papaya at room temperature (295 K). Two side peaks from the flesh at the liquid nitrogen temperature indicated a linear dose response for 3-14 days after the γ-irradiation. The line shapes recorded from the freeze-dried specimens were sharper than those at liquid nitrogen temperature.

  1. ESR detection procedure of irradiated papaya containing high water content

    ESR signals were recorded from irradiated papaya at liquid nitrogen temperature (77 K), and freeze-dried irradiated papaya at room temperature (295 K). Two side peaks from the flesh at the liquid nitrogen temperature indicated a linear dose response for 3-14 days after the γ-irradiation. The line shapes recorded from the freeze-dried specimens were sharper than those at liquid nitrogen temperature.

  2. ESR detection procedure of irradiated papaya containing high water content

    Kikuchi, Masahiro, E-mail: kikuchi.masahiro@jaea.go.j [Quantum Beam Science Directorate, Japan Atomic Energy Agency, 1233 Watanuki-machi, Takasaki, Gunma 370-1292 (Japan); Shimoyama, Yuhei [Quantum Beam Science Directorate, Japan Atomic Energy Agency, 1233 Watanuki-machi, Takasaki, Gunma 370-1292 (Japan); Ukai, Mitsuko [Hokkaido University of Education, 1-2 Hachiman-cho, Hakodate, Hokkaido 040-8567 (Japan); Kobayashi, Yasuhiko [Quantum Beam Science Directorate, Japan Atomic Energy Agency, 1233 Watanuki-machi, Takasaki, Gunma 370-1292 (Japan)

    2011-05-15

    ESR signals were recorded from irradiated papaya at liquid nitrogen temperature (77 K), and freeze-dried irradiated papaya at room temperature (295 K). Two side peaks from the flesh at the liquid nitrogen temperature indicated a linear dose response for 3-14 days after the {gamma}-irradiation. The line shapes recorded from the freeze-dried specimens were sharper than those at liquid nitrogen temperature.

  3. Complete genome sequence of an isolate of papaya leaf distortion mosaic virus from commercialized PRSV-resistant transgenic papaya in China.

    Tuo, D; Shen, W; Yan, P; Li, Ch; Gao, L; Li, X; Li, H; Zhou, P

    2013-01-01

    Papaya leaf distortion mosaic virus is highly destructive to commercial papaya production. Here, the complete genome sequence was determined for an isolate of papaya leaf distortion mosaic virus, designated PLDMV-DF, infecting the commercialized papaya ringspot virus (PRSV)-resistant transgenic papaya from China. Excluding the 3'-poly (A) tail, the sequence shares high sequence identity to several PLDMV isolates from Taiwan and Japan and is phylogenetically most closely related to the isolate from Japan. Infection of PLDMV-DF in transgenic PRSV-resistant papaya may indicate emergence of this disease in genetically engineered plants. The reported sequence for this isolate may help generate bi-transgenic papaya resistant to PRSV and PLDMV. PMID:24294960

  4. The Extraction and Properties of Carica papaya Seed Oil

    Y.M. Li

    2015-04-01

    Full Text Available The main objective of the present study was to evaluate the suitability of Ultrasound-Microwave synergistic Extraction (UMAE for the recovery of papaya seed oil as compared to Ultrasound-Assisted Extraction (UAE. The efficiency of these two methods was assessed by comparing the physicochemical properties and oxidative stability of papaya seed oil. The analytic tests were color, unsaponifiable matters, iodine value, acid value and peroxide value. The fatty acid components were analyzed by GC. Results indicated that the Ultrasound-Microwave synergistic Extraction (UMAE considerably impact on the physicochemical properties of the extracted papaya seed oil, UMAE provided papaya seed oil with obviously lighter color, lower unsaponifiable matters (2.53% and higher oxidative stability (PV, 0.98 m mol/kg than that of UAE. Analysis of fatty acid composition revealed that 13 kinds of components are identified and the total amount of fatty acids accounted for 93.13% of papaya seed oil, the predominant fatty acids in papaya seed oil were oleic (18:1, 72.60%, palmitic (16:0, 18.00%, linoleic acid (18:2, 5.80% and stearic (18:0, 3.60%.

  5. Antioxidant capacity of juice from different papaya (Carica papaya L.) cultivars grown under greenhouse conditions in Turkey

    Aysun ÖZKAN; Hamide GÜBBÜK; GÜNEŞ, Esma; ERDOĞAN, Ayşe

    2011-01-01

    The fruits of Carica papaya L. (Caricaceae) are valuable as food and are also used in traditional medicine. The present study was designed to assess the antioxidant potential of the juices of 3 papaya cultivars (PCJ): Sunrise Solo, Red Lady, and Tainung. The antioxidant capacity of PCJ obtained from fully ripened fruit was determined by the following methods: scavenging of the free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH), reducing power assay, scavenging of superoxide radicals, 2-deoxyri...

  6. Efficacy of four plant extracts on nematodes associated with papaya in Sindh, Pakistan

    This investigation examines the effect of ethanol extracts of four plant species--Azadirachta indica (neem), Withania somnifera (ashwagandha), Tagetes erecta (marigold) and Eucalyptus citriodora (eucalyptus)--against nematodes associated with papaya (Carica papaya), and it assesses their influence o...

  7. Effects of Different Concentrations and Applications of Calcium on Storage Life and Physicochemical Characteristics of Papaya (Carica Papaya L.

    T. M. M. Mahmud

    2008-01-01

    Full Text Available Papaya (Carica Papaya L. fruits index 2 were treated with 1.5, 2.5 and 3.5% solutions of calcium chloride by dipping and vacuum infiltration (-33 Kpa or untreated (0% as control. Effects of these treatments were evaluated on storage life and postharvest quality characteristics of papaya. After 21 days of storage at 13±1°C, the fruits were removed from storage for physicochemical analysis. Following additional five days holding in the storage condition for fruits used for evaluation of the rate of disease incidence and storage life. Postharvest dip treatments at different concentrations of calcium prolonged storage life, slowed down the ripening processes and maintained the quality of papaya. Whereas, it was effectively greater with calcium infiltration treatments than that for dip treatments. Calcium infiltration extended the storage life and retained the quality as calcium concentrations increased up to 2.5% and then declined. The desired effect was obtained at 2.5% infiltration compared with other treatments. The least disease incidence was found in those fruits infiltrated with 2.5% calcium. Hence, it can be concluded that postharvest infiltration of calcium at 2.5% has the potential to control disease incidence, prolong the storage life and preserve valuable attributes of postharvest papaya, presumably because of its effects on inhibition of ripening and senescence process and loss of the fruit firmness of papaya.

  8. Penentuan Dosis Insektisida Nabati Ekstrak Air Daun Pepaya (Carica papaya L.) Terhadap Larva Buah Jeruk

    Ratna

    2016-01-01

    Background: The use ofsynthetic insecticides on an ongoing basis can have a negative impact on human health and the environment. Required control alternative. Papaya leaves(Carica papaya L.)can be developedas aninsecticide active ingredient vegetable contains the enzyme papain, alkaloids, flavonoids, polyphenols, quinones, and terpenoids. Purpose: determine the effectof water extract papaya leaves mortality on larvae citrus fruit and Effective doses of water extract papaya leaves right as ...

  9. Sequences of the coat protein gene from brazilian isolates of Papaya ringspot virus

    LIMA ROBERTO C. A.; SOUZA JR. MANOEL T.; PIO-RIBEIRO GILVAN; LIMA J. ALBERSIO A.

    2002-01-01

    Papaya ringspot virus (PRSV) is the causal agent of the main papaya (Carica papaya) disease in the world. Brazil is currently the world's main papaya grower, responsible for about 40% of the worldwide production. Resistance to PRSV on transgenic plants expressing the PRSV coat protein (cp) gene was shown to be dependent on the sequence homology between the cp transgene expressed in the plant genome and the cp gene from the incoming virus, in an isolate-specific fashion. Therefore, knowledge o...

  10. 7 CFR 319.56-25 - Papayas from Central America and Brazil.

    2010-01-01

    ... 7 Agriculture 5 2010-01-01 2010-01-01 false Papayas from Central America and Brazil. 319.56-25... § 319.56-25 Papayas from Central America and Brazil. The Solo type of papaya may be imported into the... shipment to the United States in one of the following locations: (1) Brazil: State of Espirito Santo;...

  11. The dsRNA Virus Papaya Meleira Virus and an ssRNA Virus Are Associated with Papaya Sticky Disease.

    Tathiana Ferreira Sá Antunes

    Full Text Available Papaya sticky disease, or "meleira", is one of the major diseases of papaya in Brazil and Mexico, capable of causing complete crop loss. The causal agent of sticky disease was identified as an isometric virus with a double stranded RNA (dsRNA genome, named papaya meleira virus (PMeV. In the present study, PMeV dsRNA and a second RNA band of approximately 4.5 kb, both isolated from latex of papaya plants with severe symptoms of sticky disease, were deep-sequenced. The nearly complete sequence obtained for PMeV dsRNA is 8,814 nucleotides long and contains two putative ORFs; the predicted ORF1 and ORF2 display similarity to capsid proteins and RdRp's, respectively, from mycoviruses tentatively classified in the family Totiviridae. The sequence obtained for the second RNA is 4,515 nucleotides long and contains two putative ORFs. The predicted ORFs 1 and 2 display 48% and 73% sequence identity, respectively, with the corresponding proteins of papaya virus Q, an umbravirus recently described infecting papaya in Ecuador. Viral purification in a sucrose gradient allowed separation of particles containing each RNA. Mass spectrometry analysis indicated that both PMeV and the second RNA virus (named papaya meleira virus 2, PMeV2 were encapsidated in particles formed by the protein encoded by PMeV ORF1. The presence of both PMeV and PMeV2 was confirmed in field plants showing typical symptoms of sticky disease. Interestingly, PMeV was detected alone in asymptomatic plants. Together, our results indicate that sticky disease is associated with double infection by PMeV and PMeV2.

  12. The dsRNA Virus Papaya Meleira Virus and an ssRNA Virus Are Associated with Papaya Sticky Disease.

    Sá Antunes, Tathiana Ferreira; Amaral, Raquel J Vionette; Ventura, José Aires; Godinho, Marcio Tadeu; Amaral, Josiane G; Souza, Flávia O; Zerbini, Poliane Alfenas; Zerbini, Francisco Murilo; Fernandes, Patricia Machado Bueno

    2016-01-01

    Papaya sticky disease, or "meleira", is one of the major diseases of papaya in Brazil and Mexico, capable of causing complete crop loss. The causal agent of sticky disease was identified as an isometric virus with a double stranded RNA (dsRNA) genome, named papaya meleira virus (PMeV). In the present study, PMeV dsRNA and a second RNA band of approximately 4.5 kb, both isolated from latex of papaya plants with severe symptoms of sticky disease, were deep-sequenced. The nearly complete sequence obtained for PMeV dsRNA is 8,814 nucleotides long and contains two putative ORFs; the predicted ORF1 and ORF2 display similarity to capsid proteins and RdRp's, respectively, from mycoviruses tentatively classified in the family Totiviridae. The sequence obtained for the second RNA is 4,515 nucleotides long and contains two putative ORFs. The predicted ORFs 1 and 2 display 48% and 73% sequence identity, respectively, with the corresponding proteins of papaya virus Q, an umbravirus recently described infecting papaya in Ecuador. Viral purification in a sucrose gradient allowed separation of particles containing each RNA. Mass spectrometry analysis indicated that both PMeV and the second RNA virus (named papaya meleira virus 2, PMeV2) were encapsidated in particles formed by the protein encoded by PMeV ORF1. The presence of both PMeV and PMeV2 was confirmed in field plants showing typical symptoms of sticky disease. Interestingly, PMeV was detected alone in asymptomatic plants. Together, our results indicate that sticky disease is associated with double infection by PMeV and PMeV2. PMID:27166626

  13. Effect of gamma-irradiation on ripening papaya pectin

    Papaya (Carica papaya, L., var. Sunset) at three initial ripeness stages were irradiated with 0.25, 0.50, 0.75, 1.0, or 1.5 kGy gamma-irradiation and pectin changes during ripening determined. A significant linear relationship was found between irradiation dose and firmness immediately after irradiation. Irradiation had no effect on fruit skin or flesh color of papaya fruit irradiated at the 5 to 30% yellow stage and allowed to ripen. Papaya irradiated when 5 to 30% yellow showed no significant changes in pectin methylesterase activity when ripe. Immediately after irradiation, the pectin in 10 to 30% yellow papaya showed depolymerization and demethoxylation, though no effect on pectin methylesterase activity was detected. There was an increase in water soluble pectin (WSP), while chelator soluble (CSP) and alkali soluble pectin (ASP) decreased, with a significant decline in the methanol content of the ASP fraction. After the 25 to 30% yellow ripeness stage, fruit irradiated at 0.50 to 1.0 kGy had less pectic depolymerization, and had a firmer texture than nonirradiated when ripe. A lower level of WSP and higher levels of CSP and ASP were found in ripe fruit that had been irradiated at 0.5 to 1.0 kGy when 25 to 30% yellow skin with a significant quadratic relationship between irradiation dose and the three pectin fractions. The firmness of these irradiated fruit were retained for two days longer than the nonirradiated control. (author)

  14. Effect of ozone on anthracnose physicochemical responses and gene expression in papaya (carica papaya l.)

    Ong, Mei Kying

    2014-01-01

    A study was conducted to investigate the effects of varying levels of ozone (0, 1.5, 2.5, 3.5 or 5.0 ppm) for 96 h on 1. the in vitro and in vivo growth of Colletotrichum gloeosporioides, the causal organism of anthracnose; 2. the reactive oxygen species generation and spore mitochondria of C. gloeosporioides using transmission electron microscope, fluorescence microscope and laser scanning confocal microscope; 3. the production of defence-related enzymes in papaya; 4. microbiological analysi...

  15. Subchronic Immunotoxicity Assessment of Genetically Modified Virus-Resistant Papaya in Rats.

    Lin, Hsin-Tang; Lee, Wei-Cheng; Tsai, Yi-Ting; Wu, Jhaol-Huei; Yen, Gow-Chin; Yeh, Shyi-Dong; Cheng, Ying-Huey; Chang, Shih-Chieh; Liao, Jiunn-Wang

    2016-07-27

    Papaya is an important fruit that provides a variety of vitamins with nutritional value and also holds some pharmacological properties, including immunomodulation. Genetically modified (GM) papaya plants resistant to Papaya ringspot virus (PRSV) infection have been generated by cloning the coat protein gene of the PRSV which can be used as a valuable strategy to fight PRSV infection and to increase papaya production. In order to assess the safety of GM papaya as a food, this subchronic study was conducted to assess the immunomodulatory responses of the GM papaya line 823-2210, when compared with its parent plant of non-GM papaya, Tainung-2 (TN-2), in Sprague-Dawley (SD) rats. Both non-GM and GM 823-2210 papaya fruits at low (1 g/kg bw) and high (2 g/kg bw) dosages were administered via daily oral gavage to male and female rats consecutively for 90 days. Immunophenotyping, mitogen-induced splenic cell proliferation, antigen-specific antibody response, and histopathology of the spleen and thymus were evaluated at the end of the experiment. Results of immunotoxicity assays revealed no consistent difference between rats fed for 90 days with GM 823-2210 papaya fruits, as opposed to those fed non-GM TN-2 papaya fruits, suggesting that with regard to immunomodulatory responses, GM 823-2210 papaya fruits maintain substantial equivalence to fruits of their non-GM TN-2 parent. PMID:27396727

  16. Physico-chemical characteristics of papaya (Carica papaya L.) seed oil of the Hong Kong/Sekaki variety.

    Yanty, Noorzianna Abdul Manaf; Marikkar, Jalaldeen Mohammed Nazrim; Nusantoro, Bangun Prajanto; Long, Kamariah; Ghazali, Hasanah Mohd

    2014-01-01

    A study was carried out to determine the physicochemical characteristics of the oil derived from papaya seeds of the Hong Kong/Sekaki variety. Proximate analysis showed that seeds of the Hong Kong/Sekaki variety contained considerable amount of oil (27.0%). The iodine value, saponification value, unsaponifiable matter and free fatty acid contents of freshly extracted papaya seed oil were 76.9 g I2/100g oil, 193.5 mg KOH/g oil, 1.52% and 0.91%, respectively. The oil had a Lovibond color index of 15.2Y + 5.2B. Papaya seed oil contained ten detectable fatty acids, of which 78.33% were unsaturated. Oleic (73.5%) acid was the dominant fatty acids followed by palmitic acid (15.8%). Based on the high performance liquid chromatography (HPLC) analysis, seven species of triacylglycerols (TAGs) were detected. The predominant TAGs of papaya seed oil were OOO (40.4%), POO (29.1%) and SOO (9.9%) where O, P, and S denote oleic, palmitic and stearic acids, respectively. Thermal analysis by differential scanning calorimetry (DSC) showed that papaya seed oil had its major melting and crystallization transitions at 12.4°C and -48.2°C, respectively. Analysis of the sample by Z-nose (electronic nose) instrument showed that the sample had a high level of volatile compounds. PMID:25174674

  17. Simultaneous detection of papaya ringspot virus, papaya leaf distortion mosaic virus, and papaya mosaic virus by multiplex real-time reverse transcription PCR.

    Huo, P; Shen, W T; Yan, P; Tuo, D C; Li, X Y; Zhou, P

    2015-12-01

    Both the single infection of papaya ringspot virus (PRSV), papaya leaf distortion mosaic virus (PLDMV) or papaya mosaic virus (PapMV) and double infection of PRSV and PLDMV or PapMV which cause indistinguishable symptoms, threaten the papaya industry in Hainan Island, China. In this study, a multiplex real-time reverse transcription PCR (RT-PCR) was developed to detect simultaneously the three viruses based on their distinctive melting temperatures (Tms): 81.0±0.8°C for PRSV, 84.7±0.6°C for PLDMV, and 88.7±0.4°C for PapMV. The multiplex real-time RT-PCR method was specific and sensitive in detecting the three viruses, with a detection limit of 1.0×10(1), 1.0×10(2), and 1.0×10(2) copies for PRSV, PLDMV, and PapMV, respectively. Indeed, the reaction was 100 times more sensitive than the multiplex RT-PCR for PRSV, and 10 times more sensitive than multiplex RT-PCR for PLDMV. Field application of the multiplex real-time RT-PCR demonstrated that some non-symptomatic samples were positive for PLDMV by multiplex real-time RT-PCR but negative by multiplex RT-PCR, whereas some samples were positive for both PRSV and PLDMV by multiplex real-time RT-PCR assay but only positive for PLDMV by multiplex RT-PCR. Therefore, this multiplex real-time RT-PCR assay provides a more rapid, sensitive and reliable method for simultaneous detection of PRSV, PLDMV, PapMV and their mixed infections in papaya. PMID:26666186

  18. Growth of Listeria monocytogenes in melon, watermelon and papaya pulps.

    Penteado, Ana L; Leitão, Mauro F F

    2004-04-01

    Growth of Listeria monocytogenes in low-acid fruits (melon, watermelon and papaya) at different times of incubation and at temperatures of 10, 20 and 30 degrees C was studied. Fruit pulp portions with an average pH of 5.87, 5.50 and 4.87 for melon, watermelon and papaya, respectively, were obtained aseptically, homogenized, weighed and inoculated with suspensions (approximately 10(2) CFU/g) of L. monocytogenes. Generation times of 7.12, 13.03 and 15.05 h at 10 degrees C, 1.74, 2.17 and 6.42 h at 20 degrees C and 0.84, 1.00 and 1.16 h at 30 degrees C were obtained, respectively, for melon, watermelon and papaya. The results showed that L. monocytogenes grew in low-acid fruits at all tested temperatures, although growth was diminished, but not inhibited at 10 degrees C. PMID:15033271

  19. La Economia Chilena Frente a la Crisis Financiera: Respuestas Contra-Ciclicas y Desafios Pendientes

    Ricardo Ffrench-Davis; Rodrigo Heresi

    2014-01-01

    Ante el contagio de la crisis financiera internacional, la economia chilena experimento una intensa reduccion del precio del cobre y de los influjos de capitales. Consecuentemente, la demanda agregada y el empleo de trabajo y capital productivo se contrajeron hacia fines de 2008. En ese escenario, a diferencia de la reaccion frente al contagio de la crisis asiatica, la autoridad economica adopto un amplio conjunto de politicas publicas contra-ciclicas, caracterizadas principalmente por un aum...

  20. Paisajes del agua y trayectorias del arraigo en la Patagonia chilena

    Muñoz Rebolledo, Mª Dolores

    2011-01-01

    El artículo analiza las dificultades de asentarase en los paisajes de la vastedad en la Patagonia chilena, en la zona del río Baker y lago General Carrera. Toma como referencia la definicíión de paisaje contenida en el marco teórico y otros antecedentes expuestos en el capítulo 2 de la tesis doctoral

  1. Field released transgenic papaya effect on soil microbial communities and enzyme activities

    WEI Xiang-dong; ZOU Hui-ling; CHU Lee-min; LIAO Bin; YE Chang-min; LAN Chong-yu

    2006-01-01

    Soil properties, microbial communities and enzyme activities were studied in soil amended with replicase (RP)-transgenic or non-transgenic papaya under field conditions. Compared with non-transgenic papaya, significant differences (P<0.05) were observed in total nitrogen in soils grown with transgenic papaya. There were also significant differences (P<0.05) in the total number of colony forming units (CFUs) of bacteria, actinomycetes and fungi between soils amended with RP-transgenic plants and non-transgenic plants. Compared with non-transgenic papaya, the total CFUs of bacteria, actinomycetes and fungi in soil with transgenic papaya increased by 0.43-1.1, 0.21-0.80 and 0.46-0.73 times respectively. Significantly higher (P<0.05) CFUs of bacteria, actinomycetes and fungi resistant to kanamycin (Km) were obtained in soils with RP-transgenic papaya than those with non-transgenic papaya in all concentrations of Km. Higher resistance quotients for Kmr (kanam ycin resistant) bacteria, actinomycetes and fungi were found in soil planted with RP-transgenic papaya, and the resistance quotients for KTr bacteria, actinomycetes and fungi in soils with transgenic papaya increased 1.6-4.46, 0.63-2.5 and 0.75-2.30 times. RP-transgenic papaya and non-transgenic papaya produced significantly different enzyme activities in arylsulfatase (5.4-5.9x), polyphenol oxidase (0.7-1.4x), invertase (0.5-0.79x), cellulase (0.23-0.35x) and phosphodiesterase (0.16-0.2x). The former three soil enzymes appeared to be more sensitive to the transgenic papaya than the others, and could be useful parameters in assessing the effects of transgenic papaya.Transgenic papaya could alter soil chemical properties, enzyme activities and microbial communities.

  2. INFLUENCE OF THICKNESS ON THE DRYING OF PAPAYA PUREE (Carica papaya L. THROUGH REFRACTANCE WINDOWTM TECHNOLOGY

    MARÍA U. OCORÓ-ZAMORA

    2013-01-01

    Full Text Available El objetivo de este trabajo fue evaluar la influencia del espesor de las muestras (2, 3 y 4 mm sobre las cinéticas de secado, actividad de agua (aw y color (∆E de rodajas de puré de papaya procesadas por tecnología de ventana de refractanciaTM (RWTM. Adicionalmente se evaluó la difusividad del agua (Deff teniendo en cuenta el encogimiento. Los datos de humedad se ajustaron mediante los modelos de Newton y Midilli. Los resultados mostraron que a menor espesor el secado fue más rápido, los valores de aw fueron menores y los ∆E superiores. Las muestras alcanzaron 0.0652, 0.1132 y 0.2624 g agua/ g sólido seco en 60 min para rodajas de 2, 3 y 4 mm, respectivamente. El Modelo de Midilli fue el más apropiado para predecir las curvas de secado de papaya por RWTM. Deff disminuyó a menor espesor y su orden de magnitud fue de 10-10 m2/s.

  3. Sequence and genome organization of papaya meleira virus infecting papaya in Brazil.

    Abreu, Emanuel F M; Daltro, Cleidiane B; Nogueira, Elsa O P L; Andrade, Eduardo C; Aragão, Francisco J L

    2015-12-01

    Papaya sticky disease ('meleira') was first observed in Brazil at the beginning of the 1980s. The disease is characterized by intense latex exudation from the fruit surface that becomes dark as it oxidizes, which makes it difficult to sell. The causal agent, which has been called papaya meleira virus (PMeV), has been identified as an isometric virus particle, approximately 50 nm in diameter, with a double-stranded RNA genome. Here, we report the first complete sequence and organization of the 8.7-kb viral dsRNA genome. Two ORFs coding for a putative coat protein and RNA-dependent RNA polymerase (RdRp) were predicted. In silico analysis revealed that the translated ORF2 contains the conserved domains characteristic of an RdRp protein (pfam02123:RdRP 4), which is a family that includes RdRps from members of the genera Luteovirus, Totivirus and Rotavirus. Evolutionary analysis with amino acid sequences with the RdRps from members of the family Totiviridae and some dsRNA viruses showed that PMeV RdRp did not root itself in any genus. PMID:26370790

  4. NUEVO ACTIVADOR FISIOLOGICO POTENCIALIZADOR DE LA FRUCTIFICACIÓN EN PAPAYA ( Carica papaya L.

    RICARDO HERNÁNDEZ PÉREZ

    2015-12-01

    Full Text Available RESUMEN Nuevos productos son aplicados actualmente con éxito en agricultura sustentable con el fin de activar rutas metabólicas específicas en plantas, los que son conocidos comercialmente como activadores fisiológicos, desestresantes o potencializadores del rendimiento. Sin embargo, pocas investigaciones han logrado introducir en la práctica tales alternativas. En este estudio se ofrece información sobre los resultados de un nuevo formulado denominado A-CETAS/07, obtenido a partir de residuos de la industria azucarera y complementado con moléculas antioxidantes. Este se comparó con un activador comercial (testigo y se aplicó foliarmente para estimular el potencial de fructificación en papaya ( L Carica papaya., cv. Maradol roja. Los resultados evidenciaron una revigorización de las plantas, con aumento progresivo del rendimiento a medida que se incrementó la dosis de A-CETAS/07, por encima de la media histórica regional entre 64 - 74 t ha-1 a los 9 meses después del trasplante, lo cual representa una alternativa económica y con impacto favorable en el medio ambiente.

  5. Imobilisasi Enzim Papain Dari Getah Papaya Dengan Alginant

    Firman Sebayang

    2009-01-01

    Telah dilakukan isolasi enzim papain dari getah papaya (Carica papaya). Isolasi papain dalam penelitian ini menggunakan aseton 85% sebagai pengendap enzim. Setelah melalui tahap penyaringan dan pengeringan beku diperoleh papain 1,25 gram untuk setiap 50 ml getah. Imobilisasi isolat enzim papain dengan metoda penjebakan(entrapment) menggunakan alginat sebagai matrik polimer. pH optimum enzim imobil diperoleh 7,0 dan temperatur optimum 55oC dengan aktivitas enzim 55,60 unit/ml, sedangkan pH opt...

  6. Consumer in-store response to irradiated papayas

    In this study, purchase behavior of California consumers in response to irradiated papayas is described. The papayas were shipped from Hawaii and irradiated in California under a permit by the Animal and Plant Health Inspection Service and approved by the California Department of Food and Agriculture. Results show that the superior appearance of the irradiated fruit appealed to consumers and that two-thirds or more of the people queried indicated that they would buy irradiated produce. It is noted that this marketing took place in a supportive environment with no protestors present. Informational material was available

  7. Effect of maleic hydrazide and waxing on quality and shelf life of papaya (carica papaya L.) fruits

    The effect of post harvest treatment of maleic hydrazide (MH) with and with out waxing on the quality and shelf-life of Baladi and Ekostika I papaya fruits at 18 ±1°C and 85%-90% relative humidity was evaluated. Maleic hydrazide at 250 and 500 ppm significantly delayed fruit ripening by two and three days in both papaya cultivars, respectively, compared with untreated fruits. The higher the concentration, the more was the delay in fruit ripening. The results also showed that waxing addition to MH resulted in a delay of two more days in fruit ripening that treatment with MH alone. The effect of MH and waxing treatments in delaying papaya fruits ripening was manifested in retarded respiratory climacteric, reduced weight loss and delayed fruit softening and increase in total soluble solids and ascorbic acid content.(Author)

  8. Study of the extraction process of papain from latex of papaya (carica papaya l.) fruits cv. maradol

    Andrade Mahecha, Margarita M; Morales Rodríguez, Olga; Martínez Correa, Hugo A.

    2012-01-01

    In this work, we studied the extraction process of papain, present in the latex of papaya fruit (Carica papaya L.) cv. Maradol.  The variables studied in the extraction of papain were: latex:alcohol ratio (1:2.1 and 1:3) and drying method (vacuum and refractance window).  Papain enzyme responses were obtained in terms of enzymatic activity and yield of the extraction process. The best result in terms of enzyme activity and yield was obtained by vacuum drying and a latex:alcohol ratio of 1:3. ...

  9. Papaya fruit quality management during the postharvest supply chain

    Papayas are popular in tropical and subtropical regions and are being exported in large volumes to Europe, the U.S. and Japan. The fruit has excellent taste, exotic flavor and nutritional properties, being rich in vitamins A, C, and antioxidants. However, due to its highly perishable nature it has n...

  10. An ultrasonic system for determining papaya physiological properties

    Ibrahim, Sallehuddin; Ramli, Azlin; Yunus, Mohd Amri Md

    2015-05-01

    There is an increasing need for high quality fruit. As such it is important to have a fast, accurate and reliable method for measuring and monitoring the quality of fruit from the field to the consumer. This paper presents an investigation on the use of a non-destructive ultrasonic system which can be used to measure the quality of papaya.

  11. Estimation of papaya leaf area using the central vein length

    Campostrini Eliemar

    2001-01-01

    Full Text Available Four genotypes of papaya (Carica papaya L. two from the 'Solo' group (Sunrise Solo and Improved Sunrise Solo line 72/12 and two from the 'Formosa' group (Tainung 02 and Known-You 01, grown in Macaé, RJ, Brazil (lat. 22(0 24' S, long. 41(0 42' W, were used in this study. Twenty-five mature leaves from each genotype were sampled four and five months after seedling transplant to the field to determine the length of the leaf central vein (LLCV and the leaf area (LA. According to covariance analyses there were no significant differences in the slope and intercept of the mathematical models calculated for each genotype. Thus, a single mathematical model (Log LA = 0.315 + 1.85 Log LLCV, R²=0.898 was adjusted to estimate the LA using the length of LLCV for the four genotypes. An unique model can be applied to estimate the LA for the four papaya genotypes using LLCV in the range from 0.25 to 0.60 m, and for papaya trees 150 to 180 days after transplanting.

  12. 78 FR 48628 - Importation of Papayas From Peru

    2013-08-09

    .... APHIS-2012-0014, Regulatory Analysis and Development, PPD, APHIS, Station 3A-03.8, 4700 River Road Unit..., PPQ, APHIS, 4700 River Road Unit 133, Riverdale, MD 20737-1231; (301) 851- 2036. SUPPLEMENTARY... the risk associated with the importation of papayas from Central America, Brazil, Colombia,...

  13. Ekstraksi Minyak dari Biji Pepaya (Carica Papaya) dengan Metode Soxhlet Extraction dengan Mengunakan N-Butil Asetat

    Purwanto, Sigit

    2016-01-01

    Papaya seed it is a waste product from home industry. Commonly, papaya seed used to be a new plant to grow. Papaya seed have a potential as a edible oil. Soxhlet is used to obtain the oil from papaya seed by using several solvent such as: petroleum ether, methanol and n-hexane. In this research, papaya seed as raw material by using a solvent n-butyl acetate. The variables studied were the time of extraction and the ratio papaya seed to solvent . The results showed that the highest fatty acid ...

  14. Callus induction in papaya (Carica papaya L. and synseed production for low temperature storage and cryopreservation

    Teixeira da Silva Jaime A.

    2014-12-01

    Full Text Available The mid- to long-term preservation of papaya (Carica papaya L. would allow for the safeguarding of important germplasm. In this study, soft friable callus (SFC and hard callus (HC were induced from the first two true leaves of 10-day-old seedlings containing a midrib derived from the germinated seed of two cultivars (‘Rainbow’ and ‘Sunrise Solo’. Following germination on a Murashige and Skoog (MS medium that contained 3% sucrose and was free of plant growth regulators (PGRs, sections of the first true leaves from 10-day-old seedlings were exposed to seven published callus or somatic embryogenesis protocols for zygotic embryos, leaves or hypocotyls. Optimal SFC and HC induction was carried out on a half-strength MS medium following the Fitch (1993 or the Ascêncio-Cabral et al. (2008 protocol, respectively. SFC formed shoots that could then convert to plants when transferred to a full-strength MS medium devoid of PGRs. Plantlets 10-cm tall were acclimatised in two steps: first by in vitro acclimatisation in aerated vessels, the Vitron, under CO2-enriched (3000 ppm CO2, then by the transfer of individually rooted plantlets in Rockwool® blocks to a substrate of soil: pine bark : perlite (1:1:1, v/v/v. SFC and HC were then encapsulated in alginate beads, which were exposed to low temperature storage (LTS at 10°C and 15°C, and also cryopreserved for 30 days. All encapsulated alginate beads that contained SFC, HC or leaf tissue that had been stored under LTS or cryopreserved were able to regenerate callus when placed on an optimal callus induction medium. Plants derived from the control, LTS and cryopreservation protocols, either from SFC or HC, were successfully acclimatised.

  15. DESHIDRATACIÓN OSMÓTICA DE FRUTOS DE PAPAYA HAWAIANA (Carica papaya L. EN CUATRO AGENTES EDULCORANTES OSMOTIC DEHYDRATION OF HAWAIIAN PAPAYA FRUITS (Carica papaya L. USING FOUR SWEETENER AGENTS

    Margarita Maria Ríos Pérez

    2005-12-01

    Full Text Available Trozos de papaya hawaiiana (Carica papaya L. fueron sometidos a un proceso de osmo-deshidratación usando cuatro agentes edulcorantes: miel de abejas, miel de caña, crema de miel de abejas y sacarosa en medio acuoso a 79 grados Brix, temperatura de 20 ºC y 23 horas de inmersión. Los resultados estadísticos mostraron que el agente de mayor capacidad deshidratante fue la miel de abejas y el menor la sacarosa. Además, los análisis cinéticos indicaron que la máxima transferencia de masa ocurre en las primeras cuatro horas del proceso y la máxima pérdida de masa del producto que puede ser alcanzada fue de 32 % con un contenido de humedad final en los frutos de papaya osmodeshidratada de 41,3 % b.h.Pieces of Hawaiian papaya (Carica papaya L. were subjected to osmotic dehydration using four sweetener agents: honey, molasses, honey cream and sucrose in aqueous solution to 79 degrees Brix, 20 ºC temperature and 23 hours of immersion. The statistical results showed that honey was the sweetener agent with highest osmotic capacity while sucrose had the lowest. The kinetic analysis also showed that the maximum mass transfer occurs during the first four hours of the process and the maximum mass loss of the product that can be attained was 32 % with a final moisture content of 41,3 % w.b.

  16. Sensitivity of papaya seeds to gamma-irradiation

    The major problems faced by the papaya industry in Malaysia are the papaya ringspot virus disease and the short shelf-life of the fruit. Efforts have been made to address these problems and they include conventional breeding and selection and biotechnological tools for development of transgenic papayas with PRSV resistance and delayed fruit ripening characteristics. Irradiation-induced changes in papaya for selection of improved varieties could offer another approach to resolve these problems. As the first step, the radio-sensitivity of papaya seed must be established before mass irradiation of the materials to generate the desired variability for evaluation and selection later. For the first approximation dosage, the seeds of two local. varieties, Sekaki and Eksotika were irradiated as either dry seeds or as pre-germinated seeds (soaked overnight in water and surface-dried). The dosage ranged from 0 to 300 Gy. Wet pre-germinated seeds were all killed at 100 Gy while dry seeds did not show loss of viability even at 300 Gy. The dosage for dry seeds was raised to 1000 Gy in the following experiment and the LD 50 (or half-kill) was estimated to be 650 Gy. However, at this dosage, seedling growth was markedly reduced and this dosage may not be suitable for mass irradiation. From the growth data, it was estimated that 525 Gy was the dosage that reduced 50% of growth in height and this dosage was recommended for mass irradiation of dry seeds. For wet, pre-germinated seeds, results from the third experiment indicated that 42.5 Gy was the optimal dosage for mass irradiation. At this dosage, both seed germination and seedling growth was reduced by 50%. (Author)

  17. Antagonism or synergism between papaya ringspot virus and papaya mosaic virus in Carica papaya is determined by their order of infection.

    Chávez-Calvillo, Gabriela; Contreras-Paredes, Carlos A; Mora-Macias, Javier; Noa-Carrazana, Juan C; Serrano-Rubio, Angélica A; Dinkova, Tzvetanka D; Carrillo-Tripp, Mauricio; Silva-Rosales, Laura

    2016-02-01

    Antagonism between unrelated plant viruses has not been thoroughly described. Our studies show that two unrelated viruses, papaya ringspot virus (PRSV) and papaya mosaic virus (PapMV) produce different symptomatic outcomes during mixed infection depending on the inoculation order. Synergism occurs in plants infected first with PRSV or in plants infected simultaneously with PRSV and PapMV, and antagonism occurs in plants infected first with PapMV and later inoculated with PRSV. During antagonism, elevated pathogenesis-related (PR-1) gene expression and increased reactive oxygen species production indicated the establishment of a host defense resulting in the reduction in PRSV titers. Polyribosomal fractioning showed that PRSV affects translation of cellular eEF1α, PR-1, β-tubulin, and PapMV RNAs in planta, suggesting that its infection could be related to an imbalance in the translation machinery. Our data suggest that primary PapMV infection activates a defense response against PRSV and establishes a protective relationship with the papaya host. PMID:26765969

  18. Portable chlorophyll meter (PCM-502) values are related to total chlorophyll concentration and photosynthetic capacity in papaya (Carica papaya L.)

    This study was carried out to verify the practical use of the portable chlorophyll meter-PCM502 (PCM) in two papaya cultivars with contrasting green coloring of the leaf blade (‘Golden’: yellowish-green; ‘Solo’: dark green). The relationship was studied between the photosynthetic process and leaf n...

  19. Purification and characterization of a papaya (Carica papaya L.) pectin methylesterase isolated from a commercial papain preparation

    We purified a single stable pectin methylesterase (CpL-PME; EC 3.1.1.11) from a commercial papain preparation, which is isolated from Carica papaya (L.) fruit latex. This CpL-PME was separated from the abundant cysteine endopeptidases activities using sequential hydrophobic interaction and cation-ex...

  20. Chemical treatment of papaya seeds aiming at long-term storage and control of damping off

    Silvia de Carvalho Campos Botelho; Silvaldo Felipe da Silveira; Roberto Ferreira da Silva; Alexandre Pio Viana

    2014-01-01

    Damping off is a nursery disease of great economic importance in papaya and seed treatment may be an effective measure to control. The aim of this work was to evaluate the quality of papaya seeds treated with fungicides and stored under two environmental and packaging conditions. Additionally, the efficiency of fungicide treatments in the control of damping-off caused by Rhizoctonia solani was evaluated. Papaya seeds were treated with the fungicides Captan, Tolylfluanid and the mixture Tolylf...

  1. In vitro erythrocyte membrane stabilization properties of Carica papaya L. leaf extracts

    Priyanga Ranasinghe; Pathmasiri Ranasinghe; Abeysekera, W. P. Kaushalya M.; G A Sirimal Premakumara; Perera, Yashasvi S; Padmalal Gurugama; Gunatilake, Saman B.

    2012-01-01

    Background : Carica papaya L. fruit juice and leaf extracts are known to have many beneficial medical properties. Recent reports have claimed possible beneficial effects of C. papaya L. leaf juice in treating patients with dengue viral infections. This study aims to evaluate the membrane stabilization potential of C. papaya L. leaf extracts using an in vitro hemolytic assay. Materials and Methods: The study was conducted in between June and August 2010. Two milliliters of blood from healthy v...

  2. Metáforas en Lengua de Señas Chilena Metaphors in Chilean Sign Language

    Carolina Becerra

    2008-01-01

    Este estudio describe las características del lenguaje metafórico de personas sordas chilenas y su impacto en la comprensión lingüística. La relevancia de esta pregunta radica en la escasez de investigaciones realizadas, particularmente a nivel nacional. Se desarrolló un estudio cualitativo en base a análisis de videos de sujetos sordos en habla espontánea. Se confeccionó una lista de metáforas conceptuales y no conceptuales en Lengua de Señas Chilena. Posteriormente se evaluó su comprensión ...

  3. Reverse transcription loop-mediated isothermal amplification assay for rapid detection of Papaya ringspot virus.

    Shen, Wentao; Tuo, Decai; Yan, Pu; Yang, Yong; Li, Xiaoying; Zhou, Peng

    2014-08-01

    Papaya ringspot virus (PRSV) and Papaya leaf distortion mosaic virus (PLDMV), which causes disease symptoms similar to PRSV, threaten commercial production of both non-transgenic-papaya and PRSV-resistant transgenic papaya in China. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay to detect PLDMV was developed previously. In this study, the development of another RT-LAMP assay to distinguish among transgenic, PRSV-infected and PLDMV-infected papaya by detection of PRSV is reported. A set of four RT-LAMP primers was designed based on the highly conserved region of the P3 gene of PRSV. The RT-LAMP method was specific and sensitive in detecting PRSV, with a detection limit of 1.15×10(-6)μg of total RNA per reaction. Indeed, the reaction was 10 times more sensitive than one-step RT-PCR. Field application of the RT-LAMP assay demonstrated that samples positive for PRSV were detected only in non-transgenic papaya, whereas samples positive for PLDMV were detected only in commercialized PRSV-resistant transgenic papaya. This suggests that PRSV remains the major limiting factor for non-transgenic-papaya production, and the emergence of PLDMV threatens the commercial transgenic cultivar in China. However, this study, combined with the earlier development of an RT-LAMP assay for PLDMV, will provide a rapid, sensitive and cost-effective diagnostic power to distinguish virus infections in papaya. PMID:24769198

  4. Poetas mapuches en la literatura chilena Mapuche poets in Chilean literature

    Iván Carrasco M

    2000-01-01

    Full Text Available En este trabajo se estudia el modo en que las tradiciones textuales etnoliterarias de los mapuches y literarias de los españoles, se han imbricado en la literatura chilena a través del tiempo, y los aportes que ha dejado la incorporación de poetas de cultura mapuche en la poesía chilena contemporánea. En particular, se establece que autores como Sebastián Queupul, Pedro Alonzo, Elicura Chihuailaf y Leonel Lienlaf han participado en la conformación de la poesía etnocultural, dentro de la cual han propuesto una visión intercultural que sobrepasa los límites de la etnoliteratura mapuche y la literatura chilena tradicional, y un conjunto de estrategias textuales compartidas con otros poetas (enunciación sincrética, intertextualidad transliteraria y codificación plural, en la cual han creado una variedad propia, el doble registro.This paper studies the way in which mapuche textual ethnoliterary traditions and Spanish literary traditions have mixed in the Chilean literature through time, and the contribution of Mapuche poets to contemporary Chilean poetry, particularly, authors such as Sebastián Queupul, Pedro Alonzo, Elicura Chihuailaf and Leonel Lienlaf, where they have created an intercultural view that goes beyond the limits of the mapuche ethnoliterature and Chilean traditional literature, together with a bunch of text strategies shared with others poets (syncretic enunciation, transliterary intertext and plural codification, where they have created their own variety, the double register.

  5. Sequences of the coat protein gene from brazilian isolates of Papaya ringspot virus Seqüência do gene da proteína capsidial de isolados brasileiros de Papaya ringspot virus

    ROBERTO C.A. LIMA; Manoel T. Souza Jr.; GILVAN PIO-RIBEIRO; J. Albersio A. Lima

    2002-01-01

    Papaya ringspot virus (PRSV) is the causal agent of the main papaya (Carica papaya) disease in the world. Brazil is currently the world's main papaya grower, responsible for about 40% of the worldwide production. Resistance to PRSV on transgenic plants expressing the PRSV coat protein (cp) gene was shown to be dependent on the sequence homology between the cp transgene expressed in the plant genome and the cp gene from the incoming virus, in an isolate-specific fashion. Therefore, knowledge o...

  6. Cyanogenesis in glucosinolate-producing plants: Carica papaya and Carica quercifolia

    Olafsdottir, E.S.; Jørgensen, Lise Bolt; Jaroszewski, Jerzy W.

    Carica papaya, Carica quercifolia, Carica hastata, Caricaceae, Passifloraceae, Biosynthesis, Glucosinolates, Cyanohydrin glycosides, Cyanogenic glycosides, Prunasin, Tetraphyllin B, Cyclopentenylglycine......Carica papaya, Carica quercifolia, Carica hastata, Caricaceae, Passifloraceae, Biosynthesis, Glucosinolates, Cyanohydrin glycosides, Cyanogenic glycosides, Prunasin, Tetraphyllin B, Cyclopentenylglycine...

  7. Implementación del enfoque de derechos humanos: la reforma sanitaria chilena

    Nuria Cunill-Grau; Margarita Fernández

    2014-01-01

    EI propósito de este trabajo es analizar la incidencia de la arquitectura institucional publica en la implementación de las políticas públicas con enfoque de derechos humanos. Se adopta como caso de estudio la reforma sanitaria chilena a partir del año 2005, que reconoce garantías exigibles de acceso, oportunidad, calidad y protección financiera para un conjunto de problemas de salud a la ciudadanía, independientemente de su adscripción al sistema público 0 privado. La metodología integró un ...

  8. La Predicción de la Insolvencia de Empresas Chilenas

    Felipe Zurita

    2008-01-01

    Este trabajo compara modelos de inestabilidad financiera de naturaleza estadística y basados en la teoría de opciones, para el conjunto de sociedades anónimas abiertas chilenas. Los modelos estadísticos tienen un ajuste adecuado, aunque la peculiar historia de las quiebras en el período considerado, a saber, su aglomeración al inicio, pone en duda su utilidad como herramienta predictiva. En el segundo caso, en cambio, el promedio de probabilidades de quiebra muestra una alta correlación con i...

  9. EFEITOS DO CAPITAL INTELECTUAL SOBRE O DESEMPENHO FINANCEIRO EM EMPRESAS BRASILEIRAS E CHILENAS

    Salete Turra; Danielle Paná Vergini; Fellipe André Jacomossi; Nelson Hein

    2015-01-01

    O objetivo da pesquisa foi verificar a influência do Capital Intelectual medido pelo Valor Agregado do Capital Intelectual (VACI) sobre o desempenho financeiro em empresas brasileiras e chilenas no ano de 2013. Realizou-se uma pesquisa descritiva, documental, com abordagem quantitativa por meio da técnica estatística correlação canônica. Os dados foram coletados na base de dados da Tomnson®. Os resultados do estudo demonstraram correlação canônica existente entre as variáveis que compõem o VA...

  10. Referentes internacionales para el giro reformista de la izquierda chilena (1975-1990

    Marcelo Mella Polanco

    2011-01-01

    Full Text Available El presente artículo analiza el giro reformista de la izquierda chilena durante el régimen autoritario de Pinochet. Para lograrlo, se expone cómo la penetración de ideas provenientes del marxismo analítico homogeneizó un importante sector de intelectuales y expertos opositores de Pinochet, constituyendo, de esta forma, un nuevo mainstream político-académico capaz de renovar los marcos epistémicos de las ciencias sociales y diseñar la transición a la democracia.

  11. Verbo de concordancia en la lengua de señas chilena

    Dora Adamo Quintela; Irene Cabrera Ramírez; Pamela Lattapiat Navarro; Ximena Acuña Robertson

    1999-01-01

    El presente trabajo trata sobre una clase verbo de la lengua de señas chilena, el verbo de concordancia. A través de su descripción se da cuenta de los diversos mecanismos utilizados por el sistema de la lengua de señas para establecer la concordancia entre el sujeto y el objeto, que funcionan en la oración mediante el proceso de inflexión gramatical. Una característica relevante de la LSCh consiste en la utilización del espacio para producir modificaciones gramaticales regulares, entendidas ...

  12. Effect of gamma irradiation in papaya (Carica papaya L.) harvested in three degrees of maturation.; Efeito da irradiacao gama em mamao papaia (Carica papaya L.) colhido em tres pontos de maturacao

    Pimentel, Rodrigo Meirelles de Azevedo

    2001-07-01

    The papaya is a fragile tropical fruit with thin skin, susceptible to post harvest diseases and mechanical injuries. Furthermore, it is sensible to low temperatures and at normal conditions it ripens rapidly, increasing the difficulties with storage. The objective of this work was to evaluate the influence of gamma irradiation in papayas harvested in three degrees of maturation, in order to increase shelf life. To accomplish that, papayas were harvested in perfect quality conditions, washed, submitted to carnauba wax and selected by skin coloration into three distinct degrees of maturation: Mat 0, or beginning of yellow coloration; Mat 1, yellow stripes more developed, and Mat 2, one third yellow. Half of them were submitted to irradiation with 0, 75 kGy, while the others became control treatment. They were analyzed in four different periods of conservation, which were 1 day after irradiation (DAI) refrigerated (11 +- 1 deg C), 14 DAI refrigerated, 14 DAI refrigerated + 3 at room temperature (RT = 24 deg +- 2 deg C) and 14 DAI refrigerated + 6 at RT. The effect of irradiation was not influenced by the maturation degree at harvest. Irradiation promoted firmness maintenance of papaya and, therefore, delayed ripening; modified the green color of papaya to a lighter tone, which determined more homogeneity in the skin yellow color development (greater values of L* and b*), and turned the papaya flesh color lighter (rower values of b*). There was no effect of irradiation in papaya weight loss, in the occurrence of diseases, in the development of surface yellow color, in the parameter a* of papaya skin color, in the parameters L*, a* and chroma of flesh color, p H and total soluble solids content. Visual and organoleptic sensorial tests were accomplished with papayas from a new delivery in the conservation period of 14 DAI refrigerated + 3 at RT. In the visual test was evaluated the appearance of papaya in the following treatments: Mat O control, Mat O irradiated, Mat 1

  13. Transcriptome Profiling Revealed Stress-Induced and Disease Resistance Genes Up-Regulated in PRSV Resistant Transgenic Papaya

    Fang, Jingping; Lin, Aiting; Qiu, Weijing; Cai, Hanyang; Umar, Muhammad; Chen, Rukai; Ming, Ray

    2016-01-01

    Papaya is a productive and nutritious tropical fruit. Papaya Ringspot Virus (PRSV) is the most devastating pathogen threatening papaya production worldwide. Development of transgenic resistant varieties is the most effective strategy to control this disease. However, little is known about the genome-wide functional changes induced by particle bombardment transformation. We conducted transcriptome sequencing of PRSV resistant transgenic papaya SunUp and its PRSV susceptible progenitor Sunset t...

  14. 77 FR 31294 - Notice of Request for Extension of Approval of an Information Collection; Importation of Papaya...

    2012-05-25

    ... Collection; Importation of Papaya From Colombia and Ecuador AGENCY: Animal and Plant Health Inspection... with regulations for the importation of commercial shipments of fresh papaya from Colombia and Ecuador... importation of commercial shipments of fresh papaya from Colombia and Ecuador, contact Ms. Dorothy...

  15. EVALUACIÓN DE MARCADORES GENÉTICOS PARA DISCRIMINACIÓN ENTRE HEMBRAS Y HERMAFRODITAS DE PAPAYA (Carica papaya L.) VARIEDAD ‘MARADOL’

    Violeta Aspeitia-Echegaray; Ma. Alejandra Torres-Tapia; Dulce V. Mendoza-Rodríguez; M. Humberto Reyes-Valdés

    2014-01-01

    La papaya ( Carica papaya L.) presenta tres tipos sexuales: macho, hembra y hermafrodita, de los cuales solo el último posee valor comercial y calidad de exportación. Con base en su morfología, dichos tipos únicamente pueden ser identificados a partir de la floración. La segregación del sexo en esta especie se explica con un modelo de un locus multialélico, aunque tiene una base molecular más compleja ya que puede intervenir más de un gen. En la papaya ‘Maradol’ se presentan casi exclusivamen...

  16. Effects of Different Concentrations and Applications of Calcium on Storage Life and Physicochemical Characteristics of Papaya (Carica Papaya L.)

    T. M. M. Mahmud; A. Al Eryani-Raqeeb; Syed Omar, S. R.; A. R. Mohamed Zaki; Al E. Abdul-Rahman

    2008-01-01

    Papaya (Carica Papaya L.) fruits index 2 were treated with 1.5, 2.5 and 3.5% solutions of calcium chloride by dipping and vacuum infiltration (-33 Kpa) or untreated (0%) as control. Effects of these treatments were evaluated on storage life and postharvest quality characteristics of papaya. After 21 days of storage at 13±1°C, the fruits were removed from storage for physicochemical analysis. Following additional five days holding in the storage condition for fruits used for evaluation o...

  17. Purification of papain from fresh latex of Carica papaya

    Rubens Monti; Carmelita A. Basilio; Henrique C. Trevisan; Jonas Contiero

    2000-01-01

    In the present study we wish to report a method of crystallizing papain from fresh papaya latex which gave higher yields than previously reported. This method does not involve the use of sulphydryl reagents. The papain thus obtained is practically pure and shows a single band when submitted to electrophoresis on polyacrylamide gel, and is identical to the papain obtained by other methods. In routine enzymatic assays, specific activity was measured using Z-gly-pNP and BAEE as substrates. Papai...

  18. Development of transgenic papayas expressing the coat protein gene from a Brazilian isolate of Papaya ringspot virus (PRSV) = Desenvolvimento de mamoeiros transgênicos resistentes a vírus expressando o gene da capa protéica de um isolado brasileiro de Papaya ringspot virus

    Souza, M.T.; Níckel, O.; Gonsalves, D.

    2005-01-01

    Translatable and nontranslatable versions of the coat protein (cp) gene of a Papaya ringspot virus (PRSV) isolate collected in the state of Bahia, Brazil, were engineered for expression in Sunrise and Sunset Solo varieties of papaya (Carica papaya). The biolistic system was used to transform seconda

  19. Ripening behavior of papaya (Carica papaya L.) exposed to gamma irradiation

    Papaya fruit treated with 250 Gy of γ-irradiation frequently softened more uniformly than non-irradiated fruit. Fruit with less than 25% of their surface colored yellow placed immediately into storage at 10 °C after irradiation developed skin scald. This was prevented by delaying storage by 12 h. Fruit that were irradiated when 30% of the skin was yellowed softened at a slower rate than non-irradiated fruit. There was no difference in softening rate between irradiated and non-irradiated fruit at the mature green stage. Fruit stored for 14 days at 10 °C before returning to 25 °C had a slightly slower rate of softening than fruit allowed to ripen at 25 °C without storage. Premature flesh softening occurred occasionally in fruit that had between 8 and 18% of the skin yellow and 70–90% flesh coloring when irradiated. Premature softening occurred in the tests run on fruit that were harvested during the warmer months; fruit harvested during the cooler months did not show the condition. The conditions of growth that predispose fruit to more rapid softening following γ-irradiation were not determined

  20. A rapid detection for irradiated fresh papayas using ESR spectroscopy

    The detections of radicals induced in irradiated both fresh and dried papayas were carried out at liquid nitrogen and room temperature using electron spin resonance (ESR) spectroscopy. Fresh papayas irradiated by the γ-rays were separated into flesh and skin that subjected to the ESR detection. The ESR spectra of the specimen at liquid-nitrogen temperature were observed clearly at a week after the γ-irradiation. Those signals were consisted from main peak at g=2.000 and side peaks at g=2.018 and 1.982. They showed a linear response against the dose. Furthermore, the side peaks from freeze-dried papaya flesh stored at 4degC were observed clearly at two weeks after the γ-irradiation. Those signals also showed the linear dose-response. The detection scheme for irradiated fresh fruits can be done by two stages: 1) A screening test of ESR signals at liquid nitrogen temperature using fresh samples. 2) A room temperature ESR measurement using freeze-dry samples. (author)

  1. The potential of papaya leaf extract in controlling Ganoderma boninense

    Tay, Z. H.; Chong, K. P.

    2016-06-01

    Basal Stem Rot (BSR) disease causes significant losses to the oil palm industry. Numerous controls have been applied in managing the disease but no conclusive result was reported. This study investigated the antifungal potential of papaya leaf extracts against Ganoderma boninense, the causal pathogen of BSR. Among the five different solvents tested in extraction of compounds from papaya leaf, methanol and acetone gave the highest yield. In vitro antifungal activity of the methanol and acetone extracts were evaluated against G. boninense using agar dilution at four concentrations: 5 mg mL-1, 15 mg mL-1, 30 mg mL-1and 45 mg mL-1. The results indicated a positive correlation between the concentration of leaf extracts and the inhibition of G. boninense. ED50 of methanol and acetone crude extracts were determined to be 32.016 mg mL-1and 65.268 mg mL-1, respectively. The extracts were later semi-purified using solid phase extraction (SPE) and the nine bioactive compounds were identified: decanoic acid, 2-methyl-, Z,Z-10-12-Hexadecadien-1-ol acetate, dinonanoin monocaprylin, 2-chloroethyl oleate, phenol,4-(1-phenylethyl)-, phenol,2,4-bis(1-phenylethyl)-, phenol-2-(1-phenylethyl)-, ethyl iso-allocholate and 1- monolinoleoylglycerol trimethylsilyl ether. The findings suggest that papaya leaf extracts have the ability to inhibit the growth of G. boninense, where a higher concentration of the extract exhibits better inhibition effects.

  2. Efecto de la madurez, geometría y presión sobre la cinética de transferencia de masa en la deshidratación osmótica de papaya (Carica papaya L., var. Maradol)

    Lina Marcela Chavarro-Castrillón; Claudia Isabel Ochoa-Martínez; Alfredo Ayala-Aponte

    2006-01-01

    Las papayas obtenidas en el raleo (papayas menos desarrolladas entresacadas de los árboles para beneficiar el crecimiento de las otras) generalmente se descartan. Alternativamente, estas papayas pueden secarse por un proceso de deshidratación osmótica y secado térmico convencional para usarlas como snacks o como ingrediente para otros productos. Se comparó la cinética de transferencia de masa en la deshidratación osmótica de papayas de raleo frente a papayas desarrolladas con diferente grado ...

  3. Bio-safety of transgenic papaya resistant against Papaya ring spot virus%抗病毒转基因番木瓜及其安全性问题

    李世访

    2011-01-01

    本文对番木瓜生产上存在的病害问题、转基因番木瓜的研发、转基因番木瓜国际竞争的动态与趋势、转基因番木瓜的安全性、转基因木瓜的标识等几个方面进行综述.%This paper was focused on the following issues: 1) Diseases of papaya; 2) Research and development of transgenic papaya; 3) Trends of international competition on transgenic papaya; 4) Safety of transgenic papaya; 5) Identification of transgenic papaya.

  4. Effect of heat treatment on ethylene and CO2 emissions rates during papaya (Carica papaya L.) fruit ripening

    da Silva, M. G.; Santos, E. O.; Sthel, M. S.; Cardoso, S. L.; Cavalli, A.; Monteiro, A. R.; de Oliveira, J. G.; Pereira, M. G.; Vargas, H.

    2003-01-01

    Ripening studies of nontreated and treated papaya (papaya L) are accomplished by monitoring the ethylene and CO2 emission rates of that climacteric fruit, to evaluate its shelf life. The treatments simulate the commercial Phitosanitarian process used to avoid the fly infestation. Ethylene emission was measured using a commercial CO2 laser driven photoacoustic setup and CO2, using a commercial gas analysis also based on the photothermal effect. The results show a marked change in ethylene and CO2 emission rate pattern for treated fruits when compared to the ones obtained for nontreated fruits and a displacement of the climacteric pick shown that the treatment causes a decrease of shelf life of fruit.

  5. Papaya is not a host for Tomato Yellow Leaf Curl Virus

    The economic value of tomato production is threatened by tomato yellow leaf-curl virus TYLCV and its vector, the silverleaf whitefly Bemisia tabaci biotype B (Gennadius) (Hemiptera: Aleyrodidae). Use of papaya Carica papaya L. as a banker plant for a whitefly parasitoid shows promise as a whitefly m...

  6. Effect of papaya trunk angle on infestation by white peach scale, Pseudaulacaspis pentagona (Hemiptera: Diaspididae)

    Two papaya (Carica papaya L.) seedlings growing in one planting hole often results in angular or non-vertical growth of the trees. Data on trunk angularity, or leaning, (deviation from the vertical line of reference) and white peach scale, Pseudaulacaspis pentagona Targioni-Tozzetti (Hemiptera: Dias...

  7. Evaluating Hawaii-Grown Papaya for Resistance to Internal Yellowing Disease Caused by Enterobacter cloacae

    Papaya (Carica papaya L.) cultivars and breeding lines were evaluated for resistance to Enterobacter cloacae (Jordan) Hormaeche & Edwards, the bacterial causal agent of internal yellowing disease (IY), using a range of concentrations of the bacterium. Linear regression analysis was performed and IY ...

  8. The Papaya Y Chromosome Evolved Recently and Shows Gene Paucity and DNA Sequence Expansion

    Sex chromosomes in flowering plants, in contrast to those in animals, evolved relatively recently and only a few are heteromorphic. At cytological level, the sex chromosomes of papaya appear homomorphic, nevertheless, we are finding the papaya Y chromosome shows features of incipient sex chromosome ...

  9. Cloning Of Organ-Specific Genes from Papaya Using cDNA-AFLP

    Papaya is a fast growing and productive fruit crop in tropical and subtropical regions worldwide. Papaya industry has benefited from application of molecular biology and biotechnology for crop improvement, particularly for viral disease resistance using transgenic varieties. The goal of this researc...

  10. Atypical internal yellowing of papaya fruit in Hawaii caused by Enterobacter sakazakii

    Internal yellowing (IY), characterized by yellow discolored tissue around the papaya (Carica papaya) seed cavity, diffuse margins and the presence of a distinctly rotten odor, was first reported in 1987. These symptoms were associated with the causal agent Enterobacter cloacae. Here we report the fo...

  11. DEVELOPMENT, REPRODUCTION, AND SURVIVAL OF PAPAYA MEALYBUG (HOMOPTERA: PSEUDOCOCCIDAE) ON DIFFERENT HOST PLANT SPECIES

    Papaya mealybug (Paracoccus marginatus @illiams and Granara de Willink (Homoptera: Pseudococcidae)) is a polyghagus insect and a pest of various tropical crops and ornamentals. It was introduced into the United States in 1998 in Florida. Papaya mealybug potentially poses a threat to numerous agricul...

  12. Hypoglycemic effect of Carica papaya leaves in streptozotocin-induced diabetic rats

    Juárez-Rojop Isela Esther

    2012-11-01

    Full Text Available Abstract Background Traditional plant treatment for diabetes has shown a surging interest in the last few decades. Therefore, the purpose of this study was to assess the hypoglycemic effect of the aqueous extract of C. papaya leaves in diabetic rats. Several studies have reported that some parts of the C. papaya plant exert hypoglycemic effects in both animals and humans. Methods Diabetes was induced in rats by intraperitoneal administration of 60 mg/kg of streptozotocin (STZ. The aqueous extract of C. papaya was administered in three different doses (0.75, 1.5 and 3 g/100 mL as drinking water to both diabetic and non-diabetic animals during 4 weeks. Results The aqueous extract of Carica papaya (0.75 g and 1.5 g/100 mL significantly decreased blood glucose levels (pC. papaya could help islet regeneration manifested as preservation of cell size. In the liver of diabetic treated rats, C. papaya prevented hepatocyte disruption, as well as accumulation of glycogen and lipids. Finally, an antioxidant effect of C. papaya extract was also detected in diabetic rats. Conclusions This study showed that the aqueous extract of C. papaya exerted a hypoglycemic and antioxidant effect; it also improved the lipid profile in diabetic rats. In addition, the leaf extract positively affected integrity and function of both liver and pancreas.

  13. Selection and testing of epiphytic yeasts to control anthacnose in post-harvest of papaya fruit

    Capdeville, G.; Souza, M.T.; Santos, J.R.P.; Miranda, S.P.; Caetano, A.R.; Torres, F.A.G.

    2007-01-01

    Anthracnose, caused by Colletotrichum gloeosporioides, is a major post-harvest disease in papaya fruit. The major objectives of the present work were to isolate, select and test the in vitro and in vivo ability of epiphytic microorganisms, isolated from papaya fruit and leaf surfaces, in controlling

  14. A Current Overview of the Papaya meleira virus, an Unusual Plant Virus

    Paolla M. V. Abreu

    2015-04-01

    Full Text Available Papaya meleira virus (PMeV is the causal agent of papaya sticky disease, which is characterized by a spontaneous exudation of fluid and aqueous latex from the papaya fruit and leaves. The latex oxidizes after atmospheric exposure, resulting in a sticky feature on the fruit from which the name of the disease originates. PMeV is an isometric virus particle with a double-stranded RNA (dsRNA genome of approximately 12 Kb. Unusual for a plant virus, PMeV particles are localized on and linked to the polymers present in the latex. The ability of the PMeV to inhabit such a hostile environment demonstrates an intriguing interaction of the virus with the papaya. A hypersensitivity response is triggered against PMeV infection, and there is a reduction in the proteolytic activity of papaya latex during sticky disease. In papaya leaf tissues, stress responsive proteins, mostly calreticulin and proteasome-related proteins, are up regulated and proteins related to metabolism are down-regulated. Additionally, PMeV modifies the transcription of several miRNAs involved in the modulation of genes related to the ubiquitin-proteasome system. Until now, no PMeV resistant papaya genotype has been identified and roguing is the only viral control strategy available. However, a single inoculation of papaya plants with PMeV dsRNA delayed the progress of viral infection.

  15. A current overview of the Papaya meleira virus, an unusual plant virus.

    Abreu, Paolla M V; Antunes, Tathiana F S; Magaña-Álvarez, Anuar; Pérez-Brito, Daisy; Tapia-Tussell, Raúl; Ventura, José A; Fernandes, Antonio A R; Fernandes, Patricia M B

    2015-04-01

    Papaya meleira virus (PMeV) is the causal agent of papaya sticky disease, which is characterized by a spontaneous exudation of fluid and aqueous latex from the papaya fruit and leaves. The latex oxidizes after atmospheric exposure, resulting in a sticky feature on the fruit from which the name of the disease originates. PMeV is an isometric virus particle with a double-stranded RNA (dsRNA) genome of approximately 12 Kb. Unusual for a plant virus, PMeV particles are localized on and linked to the polymers present in the latex. The ability of the PMeV to inhabit such a hostile environment demonstrates an intriguing interaction of the virus with the papaya. A hypersensitivity response is triggered against PMeV infection, and there is a reduction in the proteolytic activity of papaya latex during sticky disease. In papaya leaf tissues, stress responsive proteins, mostly calreticulin and proteasome-related proteins, are up regulated and proteins related to metabolism are down-regulated. Additionally, PMeV modifies the transcription of several miRNAs involved in the modulation of genes related to the ubiquitin-proteasome system. Until now, no PMeV resistant papaya genotype has been identified and roguing is the only viral control strategy available. However, a single inoculation of papaya plants with PMeV dsRNA delayed the progress of viral infection. PMID:25856636

  16. Draft Genome Sequence of Erwinia mallotivora BT-MARDI, Causative Agent of Papaya Dieback Disease.

    Redzuan, R Ahmad; Abu Bakar, N; Rozano, L; Badrun, R; Mat Amin, N; Mohd Raih, M F

    2014-01-01

    Erwinia mallotivora was isolated from papaya trees infected with dieback disease, which were planted at the Malaysian Agricultural Research and Development Institute (MARDI), Malaysia. Here, we report a draft genome sequence of E. mallotivora BT-MARDI, which offers an important source of information for understanding pathogen and host interaction during papaya dieback development. PMID:24812220

  17. Draft Genome Sequence of Erwinia mallotivora BT-MARDI, Causative Agent of Papaya Dieback Disease

    Redzuan, R. Ahmad; Abu Bakar, N; Rozano, L.; Badrun, R.; Mat Amin, N.; Mohd Raih, M. F.

    2014-01-01

    Erwinia mallotivora was isolated from papaya trees infected with dieback disease, which were planted at the Malaysian Agricultural Research and Development Institute (MARDI), Malaysia. Here, we report a draft genome sequence of E. mallotivora BT-MARDI, which offers an important source of information for understanding pathogen and host interaction during papaya dieback development.

  18. An umbra-like virus of papaya discovered in Ecuador: detection, occurrence and phylogenetic relatedness

    Double-stranded RNA (dsRNA) extractions from papaya leaves infected with Papaya ringspot virus (PRSV) revealed the presence of an unusual 4kb band, in addition to the presumed PRSV-associated 10kb band. Partial sequence of RT-PCR products from the 4kb dsRNA revealed homology to genomes of several me...

  19. Molecular characterization and pathogenicity of Erwinia spp. associated with pineapple [Ananas comosus (L. Merr.] and papaya (Carica papaya L.

    Ramachandran Kogeethavani

    2015-12-01

    Full Text Available The Erwinia species are well-known pathogens of economic importance in Malaysia causing serious damage to high-value fruit crops that include pineapple [Ananas comosus (L. Merr.] and papaya (Carica papaya L..The 16S rRNA sequence using eubacteria fD1 and rP2 primers, identified two bacteria species; Dickeya zeae from pineapple heart rot, and Erwinia mallotivora from papaya dieback. Phylogenetic analysis based on the neighbor-joining method indicated that all the bacterial isolates clustered in their own taxa and formed monophyletic clades. From the pathogenicity test, all isolates of D. zeae and E. mallotivora showed pathogenic reactions on their respective host plants. Genetic variability of these isolates was assessed using repetitive sequence-based PCR (rep-PCR fingerprinting. The results indicated interspecies, and intraspecies variation in both species’ isolates. There were more polymorphic bands shown by rep-PCR fingerprints than enterobacterial repetitive intergenic consensus (ERIC and BOX- PCRs, however both species’ isolates produced distinguishable banding patterns. Unweighted pair-group method with arithmetic averages (UPGMA cluster analysis indicated that all Dickeya and Erwinia isolates from the same species were grouped in the same main cluster. Similarity among the isolates ranged from 77 to 99%. Sequencing of 16S rRNA using eubacteria fD1 and rP2 primers, and rep-PCR fingerprinting revealed diversity among Dickeya and Erwinia isolates. But this method appears to be reliable for discriminating isolates from pineapple heart rot and papaya dieback.

  20. Label-free quantitative proteomics reveals differentially regulated proteins in the latex of sticky diseased Carica papaya L. plants

    Silas P. Rodrigues; José A. Ventura; Aguilar, Clemente; Nakayasu, Ernesto S; Choi, Hyungwon; Sobreira, Tiago J. P.; Nohara, Lilian L.; Wermelinger, Luciana S.; Almeida, Igor C.; Zingali, Russolina B.; Fernandes, Patricia M. B.

    2012-01-01

    Papaya meleira virus (PMeV) is so far the only described laticifer-infecting virus, the causal agent of papaya (Carica papaya L.) sticky disease. The effects of PMeV on the laticifers’ regulatory network were addressed here through the proteomic analysis of papaya latex. Using both 1-DE- and 1D-LC-ESI-MS/MS, 160 unique papaya latex proteins were identified, representing 122 new proteins in the latex of this plant. Quantitative analysis by normalized spectral counting revealed 10 down-regulate...

  1. Histopathological changes in Wistar albino rats exposed to aqueous extract of unripe Carica papaya

    Taofeeq Oduola

    2010-05-01

    Full Text Available Background: Exposure of animals to xenobiotics may or may not trigger adverse response at cellular levels. Aqueous extract of unripe Carica papaya is consumed by sickle cell patients as antisickling agent in Western Nigeria. Aim: This study was undertaken to investigate the effects of Carica papaya on certain organs in Wister albino rats exposed to aqueous extract of unripe Carica papaya. Materials and Methods: Different doses of aqueous extract of unripe Carica papaya were administered orally daily for 42 days to six groups of rats. At the end of exposure, the animals were sacrificed and tissue sections were prepared from livers, kidneys, hearts and small intestines using standard techniques. Results: Histopathological results showed that no pathological changes were observed in tissue sections of experimental animals when compared with tissue sections of the same organs in control animals. Conclusion: No pathological changes were elicited in the organs of rats exposed to aqueous extract of unripe Carica papaya.

  2. Histopathological changes in Wistar albino rats exposed to aqueous extract of unripe Carica papaya

    Taofeeq Oduola

    2010-01-01

    Full Text Available Background : Exposure of animals to xenobiotics may or may not trigger adverse response at cellular levels. Aqueous extract of unripe Carica papaya is consumed by sickle cell patients as antisickling agent in Western Nigeria. Aim : This study was undertaken to investigate the effects of Carica papaya on certain organs in Wister albino rats exposed to aqueous extract of unripe Carica papaya. Materials and Methods : Different doses of aqueous extract of unripe Carica papaya were administered orally daily for 42 days to six groups of rats. At the end of exposure, the animals were sacrificed and tissue sections were prepared from livers, kidneys, hearts and small intestines using standard techniques. Results : Histopathological results showed that no pathological changes were observed in tissue sections of experimental animals when compared with tissue sections of the same organs in control animals. Conclusion : No pathological changes were elicited in the organs of rats exposed to aqueous extract of unripe Carica papaya.

  3. Geographical and Genetic Divergence Among Papaya ringspot virus Populations Within Hainan Province, China.

    Zhao, Hui; Jia, Rui Zong; Zhang, Yu-Liang; Zhu, Yun Judy; Zeng, Hui-Cai; Kong, Hua; McCafferty, Heather; Guo, An-Ping; Peng, Ming

    2016-08-01

    Papaya ringspot virus (PRSV) severely affects the global papaya industry. Transgenic papaya has been proven to have effective resistance to PRSV isolates from Hawaii, Thailand, Taiwan, and other countries. However, those transgenic cultivars failed to show resistance to Hainan Island isolates. Some 76 PRSV samples, representative of all traditional papaya planting areas across five cities (Wen Chang, n = 13; Cheng Mai, n = 14; Chang Jiang, n = 11; Le Dong, n = 25; and San Ya, n = 13) within Hainan Province, were investigated. Results revealed three genetic diversity groups (Hainan I, II, and III) that correlated with geographical distribution. Frequent mutations among PRSV isolates from Hainan were also observed. The high genetic divergence in PRSV isolates from Hainan is likely to be the cause of the failure of genetically modified papaya that targets sequence-specific virus. PMID:27070425

  4. Hurdle technology to preserve the 'Golden' papaya post harvest quality

    With the purpose of evaluating a combination of post harvest techniques on papaya Golden storage, the following treatments were investigated: carton boxes packaging (CP); CP + plastic bag of PEBD - low density polyethylene film, with 0.05mm of thickness (PE); CP + PEBD with 0.025mm of thickness impregnated with mineral ethylene scavenger (PEAbs); gamma-irradiation (0.4 kGy and 0.7 kGy); and refrigerated storage at 10 deg C e 90% of relative humidity for 2, 5, 10, 15, 20, 25, 30 and 35 days plus five days under environmental conditions to allow ripening and to simulate the product marketing. To evaluate the effects on fruit quality the following measurements were taken: - first experiment, disease incidence, skin color and loss of turgidity (as visual variables); skin and flesh color, weight loss, flesh firmness, total soluble solids, total titratable acidity, pH, lycopene and beta-carotene composition; - second experiment, sensorial analysis on samples submitted to the treatments with better results from the first experiment. Post harvest diseases were the main factor responsible for discarding fruits. Percentage of weight loss was higher on papayas stored without plastic packaging. Time affected the flesh firmness (F = 95%). However, there wasn't influence of the treatments on that parameter. A significant effect on skin color was observed with interaction between irradiation (0.4 and 0.7 kGy) and storage period. Once reached the edible stage, irradiated papayas presented more uniformity on skin color. For beta-carotene and Lycopene, the mean values were 1.27 to 1.79 and 19.16 to 23.90 mug/ml of flesh, respectively. But those substances weren't affected by the combined methods or the time. The same behavior were observed for total soluble solids, total titratable acidity and pH (mean values of 11.53 to 12.20 deg Brix, 0.117 to 0.136 g of citric acid/100g of flesh and 4.91 to 5.04, respectively). On sensory evaluation, the judges didn't get to detect significant

  5. Yellow Krang – A New Cultivar Of Papaya For Green Consumption With Tolerance To Papaya Ringspot Virus

    Janthasri Rapatsa

    2015-12-01

    Full Text Available The development of a papaya cultivar for unripe or green consumption that has resistance to papaya ringspot virus (PRSV began in 2004 by crossing red-fleshed ‘Red Krang’ cultivar with yellow-fleshed Sai Nampeung cultivar. Numerous diverse hybrids that were obtained were selected in a recurrent selection until 2010. The ’Yellow Krang’ line was selected because of the early and high yielding, elongated fruit shape, is yellow and crispy flesh when unripe and suitable for consumption as green papaya, and tolerance to PRSV. From 2010 to 2015 the ‘Yellow Krang’ line was refined through pure line selection. It was evaluated in four growing areas in Thailand for 3 years. The field trial showed that ‘Yellow Krang’ can be classified as dwarf, reaching a height of 110-150 cm, early bearing, with mean 105 days to first flower anthesis; an average of 100 flowers and 206 fruits per plant, mean fruit weight 1.2 kg, mean fruit thickness 2.4 cm, mean fruit firmness 7.15 Newtons; high yielding, with mean fresh weight yield of 134.606 kg/hectare; and 24.86% incidence of PRSV.

  6. Heat shock and gamma radiation in the quarantine process of Papaya CV. Solo(Carica papaya L.)

    Full text: To export, it is not only necessary to achieve quality standards, but also, quarantine standards. The main function of irradiation is quarantine control of fruit flies, therefore it is necessary to combine with treatments which focus at keeping fruit quality. The objective of this work was the evaluation of the quality of papaya submitted to heat treatment followed by irradiation at quarantine doses. Papayas were selected green and separated in treatments: a) control; irradiation at doses; b) 250, c) 500 Gy; d) hot water at 60 deg C for 30 seconds; and combined to doses e) 250, f) 500 Gy. The papayas were stored at 21 (± 1 deg C) with relative humidity of 85 - 90%, and then after 8 days, they were evaluated by their soluble solid content, pH, titratable acidity, firmness, decay index and internal color. The analysis of mass loss and external color were executed at 0, 2, 4 and 7 days after irradiation. It was observed that in fruits submitted to hot water (d, e, f) there were less decay and in combined treatments (e, f) it was verified higher firmness. In the second day, irradiated treatments (b, c, e, f) were more yellow than the others. As to the other parameters evaluated, there were no differences between treatments (author)

  7. Heat shock and gamma radiation in the quarantine process of Papaya CV. Solo(Carica papaya L.)

    Pimentel, Rodrigo M.A.; Walder, Julio M.M. [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil)]. E-mails: rmapiment@cena.usp.br; jmwalder@cena.usp.br; Marcondes, Yvens E.M. [Universidade Metodista de Piracicaba (UNIMEP), SP (Brazil)]. E-mail: yvensmar@unimep.br

    2007-07-01

    Full text: To export, it is not only necessary to achieve quality standards, but also, quarantine standards. The main function of irradiation is quarantine control of fruit flies, therefore it is necessary to combine with treatments which focus at keeping fruit quality. The objective of this work was the evaluation of the quality of papaya submitted to heat treatment followed by irradiation at quarantine doses. Papayas were selected green and separated in treatments: a) control; irradiation at doses; b) 250, c) 500 Gy; d) hot water at 60 deg C for 30 seconds; and combined to doses e) 250, f) 500 Gy. The papayas were stored at 21 ({+-} 1 deg C) with relative humidity of 85 - 90%, and then after 8 days, they were evaluated by their soluble solid content, pH, titratable acidity, firmness, decay index and internal color. The analysis of mass loss and external color were executed at 0, 2, 4 and 7 days after irradiation. It was observed that in fruits submitted to hot water (d, e, f) there were less decay and in combined treatments (e, f) it was verified higher firmness. In the second day, irradiated treatments (b, c, e, f) were more yellow than the others. As to the other parameters evaluated, there were no differences between treatments (author)

  8. Evaluation of Antimicrobial Properties of Edible Surface Coating Based on Carrageenan Conjugated with Silver Nanoparticles on Sekaki Papaya (Carica Papaya cv. Sekaki): A New Antimicrobial Edible Coating

    Antibacterial properties of edible surface coating based on carrageenan incorporated with silver nanoparticles (SNPs), was investigated against Escherichia coli and Staphylococcus aureus to obtain optimum concentration of SNPs. Results obtained indicate that SNPs with concentration of 40 ml L-1 effectively inhibited the growth of both Gram negative and Gram positive bacteria. Sekaki papaya fruits were then coated with prepared edible coating formulation comprising of carrageenan (0.8 % w/v) and glycerol (1.0 % w/v) with and without SNPs (40 ppm) and stored at ambient conditions (26±2 degree Celsius and 60±10 % RH). Microbial analysis of coated and uncoated papaya samples during storage indicated that the edible coating comprising of carrageenan, glycerol and SNPs, strongly inhibited the growth of fungus that caused post harvest diseases of papaya as compared to uncoated and coated papaya fruits with edible coating without SNPs. (author)

  9. Potential Test of Papaya Leaf and Seed Extract (Carica Papaya) as Larvicides against Anopheles Mosquito Larvae Mortality. SP IN Jayapura, Papua Indonesia

    Arsunan

    2015-01-01

    Anopheles mosquitoes, sp is the main vector of malaria disease that is widespread in many parts of the world including in Papua Province. There are four speciesof Anopheles mosquitoes, sp, in Papua namely: An.farauti, An.koliensis, An. subpictus, and An.punctulatus. Larviciding synthetic cause resistance. This study aims to analyze the potential of papaya leaf and seeds extracts (Carica papaya) as larvicides against the mosquitoes Anopheles sp. The experiment was conducted at the Laboratory o...

  10. An analysis on DNA fingerprints of thirty papaya cultivars (Carica papaya L.), grown in Thailand with the use of amplified fragment length polymorphisms technique.

    Ratchadaporn, Janthasri; Sureeporn, Katengam; Khumcha, U

    2007-09-15

    The experiment was carried out at the Department of Horticulture, Ubon Ratchathani University, Ubon Ratchathani province, Northeast Thailand during June 2002 to May 2003 aims to identify DNA fingerprints of thirty papaya cultivars with the use of Amplified Fragment Length Polymorphisms (AFLP) technique. Papaya cultivars were collected from six different research centers in Thailand. Papaya plants of each cultivar were grown under field conditions up to four months then leaf numbers 2 and 3 of each cultivar (counted from top) were chosen for DNA extraction and the samples were used for AFLP analysis. Out of 64 random primers being used, 55 pairs gave an increase in DNA bands but only 12 pairs of random primers were randomly chosen for the final analysis of the experiment. The results showed that AFLP markers gave Polymorphic Information Contents (PIC) of three ranges i.e., AFLP markers of 235 lied on a PIC range of 0.003-0.05, 47 for a PIC range of 0.15-0.20 and 12 for a PIC range of 0.35-0.40. The results on dendrogram cluster analysis revealed that the thirty papaya cultivars were classified into six groups i.e., (1) Kaeg Dum and Malador (2) Kaeg Nuan (3) Pakchong and Solo (4) Taiwan (5) Co Coa Hai Nan and (6) Sitong. Nevertheless, in spite of the six papaya groups all papaya cultivars were genetically related to each other where diversity among the cultivars was not significantly found. PMID:19090101

  11. An Analysis on DNA Fingerprints of Thirty Papaya Cultivars (Carica papaya L., Grown in Thailand with the Use of Amplified Fragment Length Polymorphisms Technique

    Janthasri Ratchadaporn

    2007-01-01

    Full Text Available The experiment was carried out at the Department of Horticulture, Ubon Ratchathani University, Ubon Ratchathani province, Northeast Thailand during June 2002 to May 2003 aims to identify DNA fingerprints of thirty papaya cultivars with the use of Amplified Fragment Length Polymorphisms (AFLP technique. Papaya cultivars were collected from six different research centers in Thailand. Papaya plants of each cultivar were grown under field conditions up to four months then leaf numbers 2 and 3 of each cultivar (counted from top were chosen for DNA extraction and the samples were used for AFLP analysis. Out of 64 random primers being used, 55 pairs gave an increase in DNA bands but only 12 pairs of random primers were randomly chosen for the final analysis of the experiment. The results showed that AFLP markers gave Polymorphic Information Contents (PIC of three ranges i.e., AFLP markers of 235 lied on a PIC range of 0.003-0.05, 47 for a PIC range of 0.15-0.20 and 12 for a PIC range of 0.35-0.40. The results on dendrogram cluster analysis revealed that the thirty papaya cultivars were classified into six groups i.e., (1 Kaeg Dum and Malador (2 Kaeg Nuan (3 Pakchong and Solo (4 Taiwan (5 Co Coa Hai Nan and (6 Sitong. Nevertheless, in spite of the six papaya groups all papaya cultivars were genetically related to each other where diversity among the cultivars was not significantly found.

  12. Creencias y prácticas de literacidad en familias chilenas con distintos niveles educativos

    Lissi María Rosa

    2007-01-01

    Full Text Available El estudio describe las creencias y prácticas de literacidad de familias con distinta educación en Chile. Participaron 188 niños y niñas de kindergarten y sus familias. Los padres contestaronun cuestionario acerca de su nivel educacional, ciertas prácticas de literacidcd familiar, y sus creencias acerca del desarrollo de ésta. Los resultados muestran diferencias y similitudes con resultados de países desarrollados. Las principales diferencias se dan en la frecuencia de ciertas prácticas, las que ocurren con menor frecuencia en las familias chilenas, independientemente del nivel educacional. Las principales similitudes con los hallazgos de países desarrollados se dan en las asociaciones entre el nivel educacional de la familia y las variables de literacidad familiar

  13. Interacciones tempranas y género infantil en familias monoparentales chilenas

    Marcia Olhaberry Huber

    2012-01-01

    Se realizó un estudio transversal de las interacciones madre-hijo(a) en familias monoparentales chilenas de bajo ingresos, considerando las diferencias según el género infantil. Participan 80 díadas, con 39 niñas y 41 niños entre los 4 y 15 meses y se evalúa la calidad de las interacciones con el instrumento CARE-Index. Se observan altos puntajes en depresión y estrés materno, así como un promedio en la calidad de la interacción que indica necesidad de intervención, mostrando lo anterior vuln...

  14. Curar, prevenir y asistir: Medicina y salud en la historia chilena

    Soledad Zarate C., María; del Campo P., Andrea

    2014-01-01

    Este estudio identifica y describe parte de la bibliografía disponible sobre historia de la medicina y la salud pública chilena, relativas al periodo colonial y republicano. La literatura revisada reúne títulos producidos por médicos e historiadores profesionales, comentándose los aportes temáticos y metodológicos de ambas producciones. Asimismo, el artículo consigna límites analíticos y ciertas debilidades de esta historiografía y sugiere nuevas interrogantes para futuros estudios.  This ...

  15. Implementación del enfoque de derechos humanos: la reforma sanitaria chilena

    Nuria Cunill-Grau

    2014-01-01

    Full Text Available EI propósito de este trabajo es analizar la incidencia de la arquitectura institucional publica en la implementación de las políticas públicas con enfoque de derechos humanos. Se adopta como caso de estudio la reforma sanitaria chilena a partir del año 2005, que reconoce garantías exigibles de acceso, oportunidad, calidad y protección financiera para un conjunto de problemas de salud a la ciudadanía, independientemente de su adscripción al sistema público 0 privado. La metodología integró un análisis cualitativo de normas legales, instrumentos de gestión y entrevistas en profundidad principalmente a directivos de salud. Los resultados muestran que, no obstante los evidentes logros de la reforma chilena en pos de la equidad en salud, resultan limitados sus esfuerzos de dotar de gobernanza sistémica, accountability y espacio público a su arquitectura institucional, comprometiendo con ello su intención de instaurar un enfoque de derechos humanos. Se concluye que el patrón hegemónico de diseño institucional, organización y gestión de los servicios sociales requiere ser enfrentado para construirviabilidad a la implementación de este tipo de política y se sugiere la importancia de adoptar estrategias diferenciadas de accountabitily que consideren las asimetrías sociales en la apropiación y exigibilidad de los derechos.

  16. Starch edible coating of papaya: effect on sensory characteristics

    Ariane Castricini

    2012-03-01

    Full Text Available The coating of papayas with Cassava Starch (CS and carboxymethyl starch (CMS is an alternative to extend the shelf life of these fruits. This study evaluated the effect of the three different levels of CS and CMS (1, 3, and 5% on sensory characteristics of papayas during storage. Nine selected and trained assessors evaluated 13 sensory attributes using the Multiple Comparison Test. The appearance and flavor attributes of the papayas treated with CS and CMS were compared to the control or reference sample (R - fruit without coating using a nine-point scale, which varied from 1: less intense than R; 5: equal to R; 9: more intense than R. The samples were coded with three digit numbers and evaluated with repetition by a panel of assessors. In general, appearance was more affected by the coatings than flavor. Fruits coated with 3 and 5% of both coatings kept the green color longer than the other coating’s concentrations, and at 5% the color of the fruits was less uniform on the last evaluation day. The 3 and 5% CS coating gave greater brightness to the fruits. 5% CMS favored the presence of fungi and damaged the fruit surface at the 14th day of storage. The CS coating at 5% presented peeled surface during all experimental time. Changes in fruits’ flavor were perceived at the 12th and 14th days of storage. A less characteristic flavor and a bitter taste were noticed in the fruits coated with CS and CMS at 5% at the 12th day of storage.

  17. Relaxation phenomena of radicals induced in irradiated fresh papayas

    Electron spin resonance spectrometry of the γ-irradiated fresh papayas followed by freeze-drying and powderization was performed. We found a strong single peak in the flesh was observed at g=2.004 and attributed to organic free radicals. Using the method of Lund et al., relaxation times of the peak from 0 to 14 days-stored samples after γ-irradiation were calculated. T2 showed a dose response, while T1 kept almost constant by the increment of doses. The γ-radiation-induced radicals showing progressive saturation behaviors can be caused through a different pathway from indirect effects by the low LET radiations. (author)

  18. Papaya Extract to Treat Dengue: A Novel Therapeutic Option?

    N Sarala; Paknikar, SS

    2014-01-01

    Dengue is a viral disease that today affects a vast number of people in over 125 countries and is responsible for a sizable number of deaths. In the absence of an effective antiviral drug to treat the disease, various treatments are being investigated. Studies have indicated that the juice of the leaves of the Carica papaya plant from the family Caricaceae could help to increase the platelet levels in these patients. This review describes some of the published studies on this topic. The searc...

  19. A Current Overview of the Papaya meleira virus, an Unusual Plant Virus

    Paolla M. V. Abreu; Antunes, Tathiana F. S.; Anuar Magaña-Álvarez; Daisy Pérez-Brito; Raúl Tapia-Tussell; José A. Ventura; Antonio A. R. Fernandes; Fernandes, Patricia M. B.

    2015-01-01

    Papaya meleira virus (PMeV) is the causal agent of papaya sticky disease, which is characterized by a spontaneous exudation of fluid and aqueous latex from the papaya fruit and leaves. The latex oxidizes after atmospheric exposure, resulting in a sticky feature on the fruit from which the name of the disease originates. PMeV is an isometric virus particle with a double-stranded RNA (dsRNA) genome of approximately 12 Kb. Unusual for a plant virus, PMeV particles are localized on and linked to ...

  20. Papaya pulp gelling: is it premature ripening or problems of water accumulation in the apoplast?

    Jurandi Gonçalves de Oliveira

    2010-12-01

    Full Text Available Gelled aspect in papaya fruit is typically confused with premature ripening. This research reports the characterization of this physiological disorder in the pulp of papaya fruit by measuring electrolyte leakage, Pi content, lipid peroxidation, pulp firmness, mineral contents (Ca, Mg and K - in pulp and seed tissues, and histological analysis of pulp tissue. The results showed that the gelled aspect of the papaya fruit pulp is not associated with tissue premature ripening. Data indicate a reduction of the vacuole water intake as the principal cause of the loss of cellular turgor; while the waterlogged aspect of the tissue may be due to water accumulation in the apoplast.

  1. Sales de calcio mejoran vida de anaquel y aceptabilidad general de papaya (Carica papaya L. var. Maradol fresca cortada

    Nayely Leyva López

    2011-06-01

    Full Text Available Las tendencias actuales de los consumidores por alimentos sanos y de conveniencia promueven un mayor consumo de frutas y hortalizas, donde destacan productos frescos cortados. Sin embargo, por su naturaleza estos productos pueden ser muy susceptibles a diferentes alteraciones que afectan su calidad. El uso de aditivos como sales de calcio representa una alternativa tecnológica para mantener, o incluso mejorar, la calidad durante su vida de anaquel. Por lo anterior el objetivo de esta investigación fue evaluar el efecto de sales de calcio en papaya fresca cortada sobre la calidad física, química, microbiológica, nutracéutica y sensorial. Cubos de papaya, variedad Maradol (2 cm de arista fueron tratados con lactato y cloruro de calcio al 1,0 y 3,0 %, bajo condiciones de inmersión por 2 min a 40 °C. Las muestras tratadas fueron colocadas en vasos de polietileno tereftalato (PET de 500 mL tapa, y almacenadas por 8 días a 5 °C. En comparación con los frutos testigo, los tratamientos a base de calcio demostraron mejorar la firmeza (~ 8 N, principalmente los frutos tratados con cloruro de calcio al 3,0 %. Por otra parte, la calidad nutracéutica (fenólicos totales no se vio afectada por el uso de sales de calcio, mientras que las evaluaciones microbiológicas demuestran condiciones de inocuidad conforme a normatividad. Se sugiere el uso de cloruro de calcio para fines de comercializar papaya fresca cortada.

  2. Sales de calcio mejoran vida de anaquel y aceptabilidad general de papaya (Carica papaya L. var. Maradol fresca cortada

    Nayely Leyva López

    2011-01-01

    Full Text Available Las tendencias actuales de los consumidores por alimentos sanos y de conveniencia promueven un mayor consumo de frutas y hortalizas, donde destacan productos frescos cortados. Sin embargo, por su naturaleza estos productos pueden ser muy susceptibles a diferentes alteraciones que afectan su calidad. El uso de aditivos como sales de calcio representa una alternativa tecnológica para mantener, o incluso mejorar, la calidad durante su vida de anaquel. Por lo anterior el objetivo de esta investigación fue evaluar el efecto de sales de calcio en papaya fresca cortada sobre la calidad física, química, microbiológica, nutracéutica y sensorial. Cubos de papaya, variedad Maradol (2 cm de arista fueron tratados con lactato y cloruro de calcio al 1,0 y 3,0 %, bajo condiciones de inmersión por 2 min a 40 °C. Las muestras tratadas fueron colocadas en vasos de polietileno tereftalato (PET de 500 mL con tapa, y almacenadas por 8 días a 5 °C. En comparación con los frutos testigo, los tratamientos a base de calcio demostraron mejorar la firmeza (~ 8 N, principalmente los frutos tratados con cloruro de calcio al 3,0 %. Por otra parte, la calidad nutracéutica (fenólicos totales no se vio afectada por el uso de sales de calcio, mientras que las evaluaciones microbiológicas demuestran condiciones de inocuidad conforme a normatividad. Se sugiere el uso de cloruro de calcio para fines de comercializar papaya fresca cortada.

  3. Estética, política y vida cotidiana. El caso de la pintura callejera chilena

    Patricio Rodriguez-Plaza

    2005-06-01

    Full Text Available Este artículo trata de compartir una reflexión académica con respecto al fenómeno de la pintura callejera chilena, entendiéndola como un hecho estético total, que lo es menos por sus producciones y resultados que por los usos y percepciones que lo entretejen, conforman y desparraman por la ciudad.

  4. Estética, política y vida cotidiana. El caso de la pintura callejera chilena

    Patricio Rodriguez-Plaza

    2005-01-01

    Este artículo trata de compartir una reflexión académica con respecto al fenómeno de la pintura callejera chilena, entendiéndola como un hecho estético total, que lo es menos por sus producciones y resultados que por los usos y percepciones que lo entretejen, conforman y desparraman por la ciudad.

  5. Methanolysis of Carica papaya Seed Oil for Production of Biodiesel

    Foluso O. Agunbiade

    2014-01-01

    Full Text Available The future of fossil fuel sources of energy has necessitated the need to search for renewable alternatives. Thus, Carica papaya seed oil (CPSO was employed as feedstock for the production of biodiesel by methanolysis. The seed was obtained locally, dried, and extracted with n-hexane. The CPSO was analyzed for specific gravity, viscosity, iodine value, and saponification value, among others using standard methods. The oil was transesterified by two-stage catalysis with oil to methanol mole ratio of 1 : 9. The biodiesel produced was subjected to standard fuel tests. The seed has an oil yield of 31.2% which is commercially viable. The kinematic viscosity of the oil at 313 K was 27.4 mm2s−1 while that of Carica papaya oil methylester (CPOME was reduced to 3.57 mm2s−1 and the specific gravity was 0.84 comparable with other seed-oil biodiesels and number 2 diesel. Other oil properties were compared favourably with seed oils already documented for biodiesel synthesis. CPOME’s cloud and pour points were 275 K and 274 K, respectively, and relatively higher than other biodiesels and number 2 diesel. CPOME exhibits moderate corrosion of copper strip. The methanolysis improved the fuel properties of the CPOME similar to other biodiesels. CPSO therefore exhibits a potential for biodiesel production.

  6. The treatment of paediatric burns using topical papaya.

    Starley, I F; Mohammed, P; Schneider, G; Bickler, S W

    1999-11-01

    Due to the limited resources for the management of burns in most regions of Africa there is a significant role for many aspects of traditional African medicine. The active component of many traditional preparations is often of plant origin and more than 25 plants have been described as useful in relations to burns and wound healing. Carica papaya is currently used in The Gambia at the Royal Victoria Hospital, Banjul in the Paediatric Unit as the major component of burns dressings, where it is well tolerated by the children. Cheap and widely available, the pulp of the papaya fruit is mashed and applied daily to full thickness and infected burns. It appears to be effective in desloughing necrotic tissue, preventing burn wound infection, and providing a granulating wound suitable for the application of a split thickness skin graft. Possible mechanisms of action include the activity of proteolytic enzymes chymopapain and papain, as well as an antimicrobial activity, although further studies are required. PMID:10563690

  7. Dengue fever treatment with Carica papaya leaves extracts

    Nisar Ahmad; Hina Fazal; Muhammad Ayaz; Bilal Haider Abbasi; Ijaz Mohammad; Lubna Fazal

    2011-01-01

    The main objective of the current study is to investigate the potential of Carica papaya leaves extracts against Dengue fever in 45 year old patient bitten by carrier mosquitoes. For the treatment of Dengue fever the extract was prepared in water. 25 mL of aqueous extract of C. papaya leaves was administered to patient infected with Dengue fever twice daily i.e. morning and evening for five consecutive days. Before the extract administration the blood samples from patient were analyzed. Platelets count (PLT), White Blood Cells (WBC) and Neutrophils (NEUT) decreased from 176×103/μL, 8.10×10 3/μL, 84.0% to 55×10 3/μL, 3.7×10 3/μL and 46.0%. Subsequently, the blood samples were rechecked after the administration of leaves extract. It was observed that the PLT count increased from 55×103/μL to 168×10 3/μL, WBC from 3.7×10 3/μL to 7.7×10 3/μL and NEUT from 46.0% to 78.3%. From the patient feelings and blood reports it showed that Caricapapaya leaves aqueous extract exhibited potential activity against Dengue fever. Furthermore, the different parts of this valuable specie can be further used as a strong natural candidate against viral diseases.

  8. Immobilization of mycoplana sp. MVMB2 isolated from petroleum contaminated soil onto papaya stem (carica papaya l.) and its application on degradation of phenanthrene

    Brinda Lakshmi, Mahalingam; Muthukumar, Karuppan; Velan, Manickam [Environmental Biotechnology Laboratory, Department of Chemical Engineering, A.C. College of Technology, Anna University, Chennai (India)

    2012-08-15

    This study presents the degradation of phenanthrene using immobilized Mycoplana sp. MVMB2 isolated from contaminated soil. Papaya stem pretreated by two stage processes, treating with acid or alkali and drying, was used for the immobilization of Mycoplana sp. Alkali pretreated papaya stem was found to be most effective in cell uptake compared to acid treated one. The maximum immobilization capacity at various physiochemical conditions for the alkali pretreated papaya stem was found to be at 320 min time, pH 6.5, 30 C temperature, and 18.6 x 10{sup 6} cells/mL initial concentrations. The adsorption mechanism of Mycoplana sp. MVMB2 on pretreated papaya stem was assessed using various kinetic and isotherm models. The immobilization of Mycoplana sp. MVMB2 on to pretreated papaya stem was corroborated by scanning electron microscopy and Fourier transformed IR spectroscopy analysis. The performance of immobilized cells in batch reactor showed more than 95% phenanthrene degradation within 72 h, whereas, free cells were found to require 120 h. The immobilized cells also showed better degradation performance in the packed column study. (Copyright copyright 2012 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  9. Immobilization of mycoplana sp. MVMB2 isolated from petroleum contaminated soil onto papaya stem (carica papaya l.) and its application on degradation of phenanthrene

    This study presents the degradation of phenanthrene using immobilized Mycoplana sp. MVMB2 isolated from contaminated soil. Papaya stem pretreated by two stage processes, treating with acid or alkali and drying, was used for the immobilization of Mycoplana sp. Alkali pretreated papaya stem was found to be most effective in cell uptake compared to acid treated one. The maximum immobilization capacity at various physiochemical conditions for the alkali pretreated papaya stem was found to be at 320 min time, pH 6.5, 30 C temperature, and 18.6 x 106 cells/mL initial concentrations. The adsorption mechanism of Mycoplana sp. MVMB2 on pretreated papaya stem was assessed using various kinetic and isotherm models. The immobilization of Mycoplana sp. MVMB2 on to pretreated papaya stem was corroborated by scanning electron microscopy and Fourier transformed IR spectroscopy analysis. The performance of immobilized cells in batch reactor showed more than 95% phenanthrene degradation within 72 h, whereas, free cells were found to require 120 h. The immobilized cells also showed better degradation performance in the packed column study. (Copyright copyright 2012 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  10. THE ROLE OF PAPAYA DRESSINGS IN THE MAN AGEMENT OF DIABETIC FOOT ULCERS: A PROSPECTIVE STUDY

    Bapurapu; Madipeddi; Boda; Dodda Ramesh; Sridhar Reddy; Karthik; Shankaraiah

    2015-01-01

    OBJECTIVES : To determine the role of grated papaya fruit dressings in the management of diabetic foot ulcers. STUDY DESIGN: In our study, all the patients with diabetic foot ulcers (n=94) were included. The initial management was control of hyperglycemia, surgical debridement, ant ibiotics and then wound care with the help of grated papaya dressings. In this study, dressings were changed every 24 hours. The wound was considered healthy, when it wa...

  11. Quality Characteristics, Nutraceutical Profile, and Storage Stability of Aloe Gel-Papaya Functional Beverage Blend

    Pushkala Ramachandran; Srividya Nagarajan

    2014-01-01

    Aloe vera gel, well known for its nutraceutical potential, is being explored as a functional ingredient in a wide array of health foods and drinks. Processing of exotic fruits and herbal botanicals into functional beverage is an emerging sector in food industry. The present study was undertaken to develop a spiced functional RTS beverage blend using Aloe gel (AG) and papaya. Aloe gel (30%), papaya pulp (15%), spice extract (5%), and citric acid (0.1%) were mixed in given proportion to prepare...

  12. In vitro erythrocyte membrane stabilization properties of Carica papaya L. leaf extracts

    Priyanga Ranasinghe

    2012-01-01

    Full Text Available Background : Carica papaya L. fruit juice and leaf extracts are known to have many beneficial medical properties. Recent reports have claimed possible beneficial effects of C. papaya L. leaf juice in treating patients with dengue viral infections. This study aims to evaluate the membrane stabilization potential of C. papaya L. leaf extracts using an in vitro hemolytic assay. Materials and Methods: The study was conducted in between June and August 2010. Two milliliters of blood from healthy volunteers and patients with serologically confirmed current dengue infection were freshly collected and used in the assays. Fresh papaya leaves at three different maturity stages (immature, partly matured, and matured were cleaned with distilled water, crushed, and the juice was extracted with 10 ml of cold distilled water. Freshly prepared cold water extracts of papaya leaves (1 ml containing 30 μl of papaya leaf extracts, 20 μl from 40% erythrocytes suspension, and 950 μl of phosphate buffered saline were used in the heat-induced and hypotonic-induced hemolytic assays. In dose response experiments, six different concentrations (9.375, 18.75, 37.5, 75, 150, and 300 μg/ml of freeze dried extracts of the partly matured leaves were used. Membrane stabilization properties were investigated with heat-induced and hypotonicity-induced hemolysis assays. Results: Extracts of papaya leaves of all three maturity levels showed a significant reduction in heat-induced hemolysis compared to controls (P 0.05 different from one another. Heat-induced hemolysis inhibition activity did not demonstrate a linear dose response relationship. At 37.5 μg/ml concentration of the extract, a marked inhibition of hypotonicity-induced hemolysis was observed. Conclusion: C. papaya L. leaf extracts showed a significant inhibition of hemolysis in vitro and could have a potential therapeutic effect on disease processes causing destabilization of biological membranes.

  13. Oxidative Stress in Patients with Alzheimer’s Disease: Effect of Extracts of Fermented Papaya Powder

    Mario Barbagallo; Francesco Marotta; Dominguez, Ligia J

    2015-01-01

    Brain tissue is particularly susceptible to oxidative stress (OS). Increased production of reactive oxygen species (ROS), reduced antioxidant systems, and decreased efficiency in repairing mechanisms have been linked to Alzheimer's disease (AD). Postmortem studies in AD patients' brains have shown oxidative damage markers (i.e., lipid peroxidation, protein oxidative damage, and glycoxidation). Fermented papaya (FPP, a product of Carica papaya Linn fermentation with yeast) is a nutraceutical s...

  14. Biodiesel Production by Enzymatic Transesterification of Papaya Seed Oil and Rambutan Seed Oil

    Wong, C. S.; R. Othman

    2014-01-01

    Biodiesel production from vegetable oil has gained attention as an alternative fuel to minimize the usage of fossil fuels and reduce greenhouse gases pollution. In Malaysia, oils from local fruit seeds of papaya and rambutan are potential feedstock for biodiesel production due to their high lipid contents and easily available. In the present study, papaya and rambutan seed oils were extracted via soxhlet apparatus using n-hexane and the oil yields were in between 34–40%. The extracted oils we...

  15. Antifungal Activity in Ethanolic Extracts of Carica papaya L. cv. Maradol Leaves and Seeds

    Chávez-Quintal, Pedro; González-Flores, Tania; Rodríguez-Buenfil, Ingrid; Gallegos-Tintoré, Santiago

    2011-01-01

    Bioactive compounds from vegetal sources are a potential source of natural antifungic. An ethanol extraction was used to obtain bioactive compounds from Carica papaya L. cv. Maradol leaves and seeds of discarded ripe and unripe fruit. Both, extraction time and the papaya tissue flour:organic solvent ratio significantly affected yield, with the longest time and highest flour:solvent ratio producing the highest yield. The effect of time on extraction efficiency was confirmed by qualitative iden...

  16. Pengaruh Pemeraman, Pengeringan, dan Keadaan Kulit Biji Terhadap Perkecambahan Benih Pepaya (Carica papaya L.) Varietas Red Lady

    Sebayang, Amelia

    2014-01-01

    Ripening of papaya leads to increase the rate of maturity and seed viability. Papaya seeds require light for germination. The light requirement could be supplied with drying before the seeds were planted. There is a phenolic compound in sarcotesta surrounding the papaya seed which act as a germination inhibitor. This experiment was conducted in the Seed Technology Laboratory, Agriculture Faculty, University of Sumatera Utara during Juli-September 2013, using a factorial randomized block desi...

  17. Variation in the Coat Protein Gene of Papaya ringspot Virus Isolates from Multiple Locations of China

    2007-01-01

    The potyvirus Papaya ringspot virus (PRSV) is an important pathogen of papaya that causes severe losses in economic crops for papaya production globally. The coat protein (CP) genes of five PRSV isolates originating from different locations in China were cloned and sequenced. The CP-coding region varied in size from 864-873 nucleotides, encoding proteins of 288-291 amino acids. The five Chinese isolates of PRSV have been characterized as papaya-infecting (PRSV-P). The CP sequences of the Chinese isolates were compared with those of previously published PRSV isolates originating from different countries at amino acid levels. A number of KE repeat boxes in the N terminus of the PRSV-CP were found in all Chinese isolates. The phylogenetic branching pattern revealed that there was certain extended grouping between geographic locations, and the Asian type probably represents the oldest population of PRSV. The information of CP genes will be useful in designing and developing durable virus resistant-PRSV transgenic papaya in China. Meanwhile broad-spectrum-virus resistant, strongly resistant-PRSV and good safe papaya lines are required.

  18. Oral dosing with papaya latex is an effective anthelmintic treatment for sheep infected with Haemonchus contortus

    Donnan Alison A

    2011-03-01

    Full Text Available Abstract Background The cysteine proteinases in papaya latex have been shown to have potent anthelmintic properties in monogastric hosts such as rodents, pigs and humans, but this has not been demonstrated in ruminants. Methods In two experiments, sheep were infected concurrently with 5,000 infective larvae of Haemonchus contortus and 10,000 infective larvae of Trichostrongylus colubriformis and were then treated with the supernatant from a suspension of papaya latex from day 28 to day 32 post-infection. Faecal egg counts were monitored from a week before treatment until the end of the experiment and worm burdens were assessed on day 35 post-infection. Results We found that the soluble fraction of papaya latex had a potent in vivo effect on the abomasal nematode H. contortus, but not on the small intestinal nematode T. colubriformis. This effect was dose-dependent and at tolerated levels of gavage with papaya latex (117 μmol of active papaya latex supernatant for 4 days, the H. contortus worm burdens were reduced by 98%. Repeated treatment, daily for 4 days, was more effective than a single dose, but efficacy was not enhanced by concurrent treatment with the antacid cimetidine. Conclusions Our results provide support for the idea that cysteine proteinases derived from papaya latex may be developed into novel anthelmintics for the treatment of lumenal stages of gastro-intestinal nematode infections in sheep, particularly those parasitizing the abomasum.

  19. Gamma radiation use as a quarantine treatment for Ceratitis capitata (Wied., 1824) and Anastrepha fraterculus (Wied., 1830) in papaya fruits (Carica papaya, Linnaeus), cultivar sunrise solo

    The gamma irradiation as treatment for control of the immature stages of fruit flies, C. capitata and A. fraterculus in papaya fruits, cultivar Sunrise Solo is studied. The gamma rays attenuation was observed by one and two papaya fruits placed between source and infested fruit, and this changed the radiation doses required to obtain mortality in eggs and larvae of the Medfly. The efficacy of irradiation was higher when the infested papayas were packed in paper box. The radiation dose determined using the Probit 9 concept, for immature stages mortality of C. capitata was 107,74 Gy and for A. fraterculus was 50,82 Gy. The sterilizing doses for adults of C. capitata and D. fraterculus, irradiated as pupae, was 60 Gy and 40 Gy, respectively. (author)

  20. Evaluación de híbridos de papaya (carica papaya l. en pococí, limón, Costa Rica

    Eric Mora

    2004-01-01

    Full Text Available Evaluación de híbridos de papaya (Carica papaya L. en Pococí, Limón, Costa Rica. Se estableció un experimento con nueve híbridos de papaya con el objetivo de determinar sus potenciales agronómicos y comerciales en una región de alta pluviosidad (cantón de Pococí, provincia de Limón. Las características evaluadas fueron tamaño de fruta, productividad total, brix de la pulpa y susceptibilidad a la antracnosis de la fruta causado por Colletotrichum gloesporioides. Se determinó que solo uno de los materiales mostró características similares al testigo comercial como fruta para consumo en fresco. Otro de los híbridos tuvo potencial para uso con fines agroindustriales.

  1. L-ascorbic acid, β-carotene and lycopene content in papaya fruits (Carica papaya) with or without physiological skin freckles Conteúdo de ácido l-ascórbico, β-caroteno e licopeno em frutos de mamão (Carica papaya) com e sem mancha fisiológica

    Leandro Marelli de Souza; Karla Silva Ferreira; José Benício Paes Chaves; Sílvio Lopes Teixeira

    2008-01-01

    The Skin Freckles is a papaya skin disorder that depreciates de fruit appearance and hampers its commercialization, although not lowering its nutritive value. Being the papaya a good source of ascorbic acid, β-carotene and licopene this research aimed at determining L-ascorbic acid, β-carotene and licopene content in papaya fruits, from 'Formosa' and 'Solo' group varieties, with and without apparent physiological skin disease (skin freckles). Fruits were harvested in the Southeast R...

  2. Indução de brotos laterais de mamoeiro (Carica papaya L. Induction of lateral shoots of papaya tree (Carica papaya L.

    José Segundo Giampan

    2005-04-01

    Full Text Available O presente trabalho teve por objetivo estudar o efeito da benzilaminopurina e do ácido giberélico, bem como a forma de aplicação destes, na produção de brotos laterais de mamoeiro. Foram utilizados mamoeiros 'Sunrise Solo' e os reguladores vegetais benzilaminopurina (BAP e ácido giberélico (GA, na concentração de 500 mg.L-1. As formas de aplicação de BAP + GA estudadas foram: pulverização; pasta de lanolina; injeção; completo, que consistiu na utilização de todas as formas de aplicação, e o controle, constituído pela ausência de aplicação de reguladores. Foram realizadas três aplicações semanais dos reguladores em cada forma de aplicação, sendo eliminado o meristema apical de todas as plantas após a última aplicação. O comprimento e o diâmetro dos brotos laterais foram avaliados aos 30; 55 e 86 dias após a retirada da gema apical. O uso de reguladores favoreceu a produção de brotos laterais. A pulverização apresentou os melhores resultados tanto em relação ao número de brotos obtidos quanto à conformação das estacas, seguida pela injeção e pasta de lanolina.The aim of the present work was to evaluate the effect of 6-benzylaminopurine (BAP and gibberellic acid (GA, as well as the form of application of these growth regulators in the plant, for the production of lateral shoots on papaya trees. Twelve months old 'Sunrise Solo' papaya trees were treated with BAP and GA, in the concentration of 500 mg.L-1. BAP + GA were applied to the trees by means of spraying, lanolin paste, injection, and a combination of all three procedures (complete. Untreated plants were used as control. After three successive applications of the plant growth regulators in each form of application, at one week intervals, all plants were decapitated. The length and diameter of the lateral shoots were measured at 30, 55 and 86 days after decapitation. The use of plant growth regulators favored the production of lateral shoots. The

  3. Heat treatment and gamma radiation on the control of Colletotrichum gloeosporioides (Penz.) Penz. et Sacc., causal agent of the papaya fruits (Carica papaya L.) anthracnose

    For controlling papaya anthracnose on stored fruits the effect of heat treatment and gamma radiation on Colletotrichum gloeosporioides was studied in vitro and in vivo. Some physiological studies with four isolades of C. gloeosporioides from papaya fruits concerning the best conditions for mycelial growth and sporulation were also performed. The results showed that the best conditions for isolates development in BDA were: temperature between 25 and 270 C for mycelium radial growth; between 27 and 300 C under 12 hours alternating periods of fluorescent). (author). 131 refs., 9 figs., 33 tabs

  4. Morfología de la flor y de la semilla de papaya (carica papaya l.): variedad maradol e híbrido tainung-1

    Gil, Arlette Ivonne; Miranda, Diego

    2010-01-01

    Con el objetivo de analizar la morfología de la flor y de la semilla de papaya (Carica papaya L.) de la variedad ‘Maradol’ y el híbrido ‘Tainung-1’, se recolectó el material vegetal en dos plantaciones y se llevaron al laboratorio de Fisiología de Cultivos, Facultad de Agronomía, Universidad Nacional de Colombia, Bogotá, con el fin de realizar las descripciones correspondientes de los tres tipos de flores (femeninas, hermafroditas y estaminadas) y de la semilla, sus características externas (...

  5. Control de dos especies de Colletotrichum causantes de antracnosis en frutos de papaya Maradol Control of two species of Colletotrichum causing anthracnose in Maradol papaya fruits

    Felipe Santamaría Basulto; Raúl Díaz Plaza; Omar Gutiérrez Alonso; Jorge Santamaría Fernández; Alfonso Larqué Saavedra

    2011-01-01

    La antracnosis es una limitante de la vida útil de los frutos de papaya, es causada principalmente por Colletotrichum gloeosporioides, aunque es posible encontrar otras especies de Colletotrichum causando enfermedades en un mismo cultivo. Para el control de la antracnosis en Yucatán se recomiendan varios fungicidas, pero no todos están permitidos por la agencia de protección al ambiente (EPA) de los Estados Unidos de América, principal país al cual se exporta la papaya yucateca. El presente e...

  6. Empleo del método de secado convectivo combinado para la deshidratación de papaya (Carica papaya L.), variedad Maradol roja

    Sahylin Muñiz Becerá; Antihus Hernández Gómez; Annia García Pereira; Lilia Méndez Lagunas

    2013-01-01

    La presente investigación tiene como objetivo evaluar el método de secado convectivo de papaya (Carica papaya L.) variedad Maradol roja, combinado con la aplicación de pretratamientos de osmosis (DOSC) y escaldado simple (ESSC), mediante el efecto de los factores tecnológi- cos del secador: temperatura (40 y 60oC) y velocidad del flujo de aire (2,5 y 1,5 m/s) sobre el comportamiento de las propiedades de calidad de la fruta deshidratada y la cinética del proceso. El pretratamiento de escaldad...

  7. Pengaruh Konsentrasi Natrium Klorida (NaCl) dan Lama Perendaman Buffer Fosfat Terhadap Perolehan Crude Papain Dari Daun Papain (Carica Papaya, L.)

    Hasibuan, Pinta Rizki Mala

    2016-01-01

    Papaya (Carica papaya L.) is one of the fruits of commodities internationally, either in the form of fresh fruit or as processed products. The leaves are green still not fully utilized. In papaya enzyme papain which allegedly contained can be used as a meat tenderizer. Papain is a protease enzyme contained in papaya latex, whether in fruit, stems and leaves, as an enzyme capable of solving the protein molecules, current papain into products that are beneficial to human life, either at home or...

  8. Identification of gamma-irradiated papaya, melon and watermelon

    Marín-Huachaca, Nélida S.; Mancini-Filho, Jorge; Delincée, Henry; Villavicencio, Anna Lúcia C. H.

    2004-09-01

    Ionizing radiation can be used to control spoilage microorganisms and to increase the shelf life of fresh fruits and vegetables in replacement for the treatment with chemical fumigants. In order to enforce labelling regulations, methods for detecting the irradiation treatment directly in the produce are required. Recently, a number of detection methods for irradiated food have been adopted by the Codex Comission. A rapid screening method for qualitative detection of irradiation is the DNA Comet Assay. The applicability of the DNA Comet Assay for distinguishing irradiated papaya, melon, and watermelon was evaluated. The samples were treated in a 60Co facility at dose levels of 0.0, 0.5, 0.75, and 1.0kGy. The irradiated samples showed typical DNA fragmentation whereas cells from non-irradiated ones appeared intact. In addition to the DNA Comet Assay also the half-embryo test was applied in melon and watermelon to detect the irradiation treatment.

  9. Identification of gamma-irradiated papaya, melon and watermelon

    Marin-Huachaca, N.S.; Mancini-Filho, Jorge E-mail: jmancini@usp.br; Delincee, Henry E-mail: henry.delincee@bfe.uni-karlsruhe.de; Villavicencio, A.L.C.H. E-mail: villavic@net.ipen.br

    2004-10-01

    Ionizing radiation can be used to control spoilage microorganisms and to increase the shelf life of fresh fruits and vegetables in replacement for the treatment with chemical fumigants. In order to enforce labelling regulations, methods for detecting the irradiation treatment directly in the produce are required. Recently, a number of detection methods for irradiated food have been adopted by the Codex Comission. A rapid screening method for qualitative detection of irradiation is the DNA Comet Assay. The applicability of the DNA Comet Assay for distinguishing irradiated papaya, melon, and watermelon was evaluated. The samples were treated in a {sup 60}Co facility at dose levels of 0.0, 0.5, 0.75, and 1.0 kGy. The irradiated samples showed typical DNA fragmentation whereas cells from non-irradiated ones appeared intact. In addition to the DNA Comet Assay also the half-embryo test was applied in melon and watermelon to detect the irradiation treatment.

  10. Identification of gamma-irradiated papaya, melon and watermelon

    Ionizing radiation can be used to control spoilage microorganisms and to increase the shelf life of fresh fruits and vegetables in replacement for the treatment with chemical fumigants. In order to enforce labelling regulations, methods for detecting the irradiation treatment directly in the produce are required. Recently, a number of detection methods for irradiated food have been adopted by the Codex Comission. A rapid screening method for qualitative detection of irradiation is the DNA Comet Assay. The applicability of the DNA Comet Assay for distinguishing irradiated papaya, melon, and watermelon was evaluated. The samples were treated in a 60Co facility at dose levels of 0.0, 0.5, 0.75, and 1.0 kGy. The irradiated samples showed typical DNA fragmentation whereas cells from non-irradiated ones appeared intact. In addition to the DNA Comet Assay also the half-embryo test was applied in melon and watermelon to detect the irradiation treatment

  11. Verbo de concordancia en la lengua de señas chilena

    Dora Adamo Quintela

    1999-09-01

    Full Text Available El presente trabajo trata sobre una clase verbo de la lengua de señas chilena, el verbo de concordancia. A través de su descripción se da cuenta de los diversos mecanismos utilizados por el sistema de la lengua de señas para establecer la concordancia entre el sujeto y el objeto, que funcionan en la oración mediante el proceso de inflexión gramatical. Una característica relevante de la LSCh consiste en la utilización del espacio para producir modificaciones gramaticales regulares, entendidas como procesos de inflexión, a través de la co-ocurrencia de parámetros gestual-visuales, que permiten una comunicación eficiente entre sus usuarios. (This article focuses on a particular type of verb used in Chilean Sign Language: the verb of agreement. Through its description, different mechanisms used to show subject-object accord operating through inflection are presented. A relevant feature of Chilean Sign Language (LSCh, is to use space to mark regular grammatical modifications, understood as processes of inflection through the co-occurrence of visual-kinetic parameters that allow its user an efficient communication.

  12. LOS DERECHOS SOCIALES DE PRESTACIÓN EN LA JURISPRUDENCIA CHILENA

    José Ignacio Martínez Estay

    2010-01-01

    Full Text Available Parte importante de los derechos sociales consisten en prestaciones. Desde el punto de vista jurídico ello parece ser una limitante para su garantía y satisfacción, pues dependen de los recursos económicos de que disponga el Estado. Por eso, lo usual es que si se constitucionalizan no llevan aparejada una garantía jurisdiccional. Así ocurre en la Constitución chilena. Dicha limitación se ve ratificada por la jurisprudencia de la Corte Suprema, que reiteradamente ha negado lugar a recursos de protección en que se intentaba la tutela de derechos sociales consistentes en prestaciones, vinculándolos a algún derecho protegido jurisdiccionalmente. Pero la reciente jurisprudencia del Tribunal Constitucional respecto del derecho a la protección de la salud y del derecho a la seguridad social contradice lo anterior, atribuyéndole a los derechos sociales consistentes en prestaciones el mismo valor que el de los demás derechos y libertades.

  13. Perfil bibliométrico de la odontología chilena, 2001-2010 Bibliometric profile of chilean dentistry, 2001-2010

    R Cartes-Velásquez; P Aravena Torres

    2012-01-01

    Introducción: Caracterizar la producción científica personal, institucional o nacional es una necesidad creciente para comprender y mejorar la generación de conocimiento. El objetivo de este trabajo es definir el perfil bibliométrico de la odontología chilena en la última década. Metodología: En la plataforma Web of Knowledge, mediante un algoritmo se realizó la búsqueda de las publicaciones odontológicas chilenas ISI. Los datos: número de publicaciones; factor de impacto (FI); Top-10 de: aut...

  14. Study about papaya (Carica papaya L.) conservation submitted to a combined treatment of irradiation and an edible film of quitosan

    In this research, on a first stage the effect of two treatments were studied: thermal, and a chitosan edible coating, combined with four different irradiation doses: 0,00 kGy, 0,50 kGy, 0,75 kGy and 1,00 kGy for effects in the ripening of papaya (Carica Papaya) cv. Golden. To evaluate the influence of these two factors in the physical-chemical characteristics of the fruit during ripening, analysis on color, both skin and pulp, firmness, soluble solids, pH and titrable acidity were performed. The incidence of fungi in fruit skin was also analyzed. The fruits were first maintained at 10 deg C chamber for fifteen days to simulate maritime transportation to the importer country and then transferred to a 25 deg C chamber aiming simulation of the commercialization conditions. From this first step, the conclusion was that chitosan in the concentration of 2% was not able to contain the fungi development and that it also influenced in a significant form the variable pH. A more expressive result was obtained for irradiation doses of 0,75 kGy and 1,00 kGy, as it was observed in the beginning the acceleration in ripening through the variables skin color and penetration energy of the fruit pulp. When storage temperature was changed to 25 deg C this situation inverted for these same variables and a two day delay was observed on the development of yellow color on the fruit skin and loss of pulp firmness. The variables titrable acidity, soluble solids and pulp color were not influenced by treatment or by irradiation. On a second stage, the fruits, in the same condition of the previous stage, were analyzed in relation of their mass and ripening stage using a ripening scale provided by the producer of the fruits for this experiment, as to evaluate the gradual development of fruit ripening according to the various treatments (chitosan 3%, thermal or control) and irradiation dose (0,00 kGy and 0,75 kGy). Confirming the results obtained in the first stage, the irradiation was able to

  15. JOAQUÍN EDWARDS BELLO: CRÓNICA Y CRÍTICA DE LA VIDA COTIDIANA CHILENA

    Leónidas Morales T

    2009-04-01

    Full Text Available Si bien el objeto central del artículo es la crítica a la vida cotidiana chilena en Joaquín Edwards Bello, en su primera parte se detiene en algunos aspectos importantes del género en que la crítica se da, la crónica urbana, asociados a su origen periodístico, a su estructura, a su lector y al estado de la modernidad chilena que su irrupción a comienzos del XX implica. En la segunda parte, y sobre la base de un breve corpus cronístico, el artículo intenta definir lo esencial de la crítica de Edwards como una crítica a un modo específico (no interrumpido desde la Colonia de ejercerse el poder en la sociedad chilena, uno de extrema polarización, y a los discursos ideológicos que le prestan la cobertura de su "naturalización".This article focuses on the criticism of Chilean everyday life in the writings of Joaquín Edwards Bello. The first part concentrates on important aspects of urban chronicle as a genre associated to its journalistic origin, structure, readership, and the conditions of Chilean modernity at the beginning of the 20th century. Supported by a brief corpus of chronicles, the second part of the article attempts to define one essential feature present in Edwards's criticism: namely a criticism of both a specific and uninterrupted mode in which power has been imposed on the Chilean society effecting extreme forms of polarization and the ideological discourses that "naturalize" its presence.

  16. Relationships between SAP-flow measurements, whole-canopy transpiration and reference evapotranspiration in field-grown papaya (Carica papaya L.)

    Whole-canopy gas exchange measurement in papaya can provide a scientific basis to optimize irrigation, and fruit yield and quality. The objectives of this study were to: 1) verify the relationship between xylem sap flow measured by the heat coefficient method and whole canopy transpiration in ‘Gra...

  17. Low-dose gamma irradiation following hot water immersion of papaya (Carica papaya linn.) fruits provides additional control of postharvest fungal infection to extend shelf life

    Low-dose gamma irradiation (0.08 kGy over 10 min), a level significantly below that required to satisfy the majority of international quarantine regulations, has been employed to provide a significant reduction in visible fungal infection on papaya fruit surfaces. This is appropriate for local and national markets in producer countries where levels of commercial acceptability can be retained despite surface lesions due to fungal infection. Irradiation alone and in combination with hot-water immersion (50 °C for 10 min) has been applied to papaya (Carica papaya L.) fruits at both the mature green and 1/3 yellow stages of maturity. The incidence and severity of surface fungal infections, including anthracnose, were significantly reduced by the combined treatment compared to irradiation or hot water treatment alone, extending storage at 11 °C by 13 days and retaining commercial acceptability. The combined treatment had no significant, negative impact on ripening, with quality characteristics such as surface and internal colour change, firmness, soluble solids, acidity and vitamin C maintained at acceptable levels. - Highlights: • Storage of papaya extended to 28 days whilst retaining commercial quality. • Additive effect of low gamma irradiation (0.08 kGy over 10 min) and hot-water treatment. • Significant reduction in surface fungal lesions. • No significant impact on colour change or flesh quality during storage

  18. Molecular diagnosis of Papaya meleira virus (PMeV) from leaf samples of Carica papaya L. using conventional and real-time RT-PCR.

    Abreu, Paolla M V; Piccin, João G; Rodrigues, Silas P; Buss, David S; Ventura, José A; Fernandes, Patricia M B

    2012-03-01

    Papaya meleira virus (PMeV) is the causal agent of papaya sticky disease. This study describes two methods for molecular diagnosis of PMeV using conventional and real-time PCR. These methods were shown to be more efficient than current methods of viral detection using extraction of PMeV dsRNA and observation of symptoms in the field. The methods described here were used to evaluate the effect of inoculation of papaya plants with purified PMeV dsRNA on the progress of PMeV infection. A single inoculation with PMeV dsRNA was observed to delay the progress of the virus infection by several weeks. The possibility of vertical transmission of PMeV was also investigated. No evidence was found for PMeV transmission through seeds collected from diseased fruit. The implications of these results for the epidemiology of PMeV and the management of papaya sticky disease are discussed. PMID:22193169

  19. Production of Internal Yellowing Symptoms on Resistant and Susceptible Papaya Cultivars by Enterobacter cloacae at Varying Inoculum Concentrations

    Internal yellowing (IY) is a bacterial disease of ripening papaya flesh caused by Enterobacter cloacae and characterized by yellow softening tissue. IY restricts food safety of value-added products like fresh or frozen papaya cubes. The incidence of E. cloacae presumably differs in resistant (R) a...

  20. 40 CFR 174.515 - Coat Protein of Papaya Ringspot Virus; exemption from the requirement of a tolerance.

    2010-07-01

    ...; exemption from the requirement of a tolerance. Residues of Coat Protein of Papaya Ringspot Virus are exempt... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Coat Protein of Papaya Ringspot Virus; exemption from the requirement of a tolerance. 174.515 Section 174.515 Protection of...

  1. 76 FR 49725 - Notice of Decision To Authorize the Importation of Fresh Papaya Fruit From Malaysia into the...

    2011-08-11

    ... United States of fresh papaya fruit (Carica papaya) from Malaysia. The pest risk analysis consisted of a... the findings of a pest risk analysis, which we made available to the public for review and comment... Federal Register announcing the availability of a pest risk analysis that evaluates the risks...

  2. DE ARMARIOS Y BIBLIOTECAS: MASCULINIDAD Y TRADICIÓN LITERARIA CHILENA EN LA NARRATIVA DE ALBERTO FUGUET

    Cristian Opazo

    2009-01-01

    La tesis de este ensayo postula que la narrativa de Alberto Fuguet puede ser leída como un proyecto escriturario que propone una (nueva) estrategia retórica por la cual representar los desvíos del género sexual. Según este modus operandi, en las narraciones de Fuguet, tanto el acto de reprimir como el de explicitar los desvíos de la masculinidad, se ejecutan a través de un juego de citas a la tradición literaria chilena. En los textos de José Donoso, Jorge Edwards y Alfredo Gómez Morel, entre...

  3. Interculturalidad y ciencias de la educación en lengua de señas chilena

    Carolina-Alejandra-de-Lourdes Becerra-Sepúlveda

    2013-01-01

    Los paradigmas de intervención orales para la cultura sorda afectan la percepción sobre la lengua de señas Chilena . Esto provoca debates emanados de una subvaloración, dadas sus particularidades visuales: iconicidad e isomorfismo, subvaloración nacida de una incomprensión en la lectura de investigaciones internacionales (por escasez de estudios nacionales), especialmente acerca del lenguaje corporeizado. Esto se contradice con nuevas investigaciones en psicolingüística cognitiva acer-ca de e...

  4. Incorporación del paisaje en la gestión del territorio de la Patagonia Chilena

    Maria Dolores Muñoz; Gerardo Azócar

    2014-01-01

    Este artículo presenta una metodología para el análisis y gestión del paisaje en la Patagonia chilena, un territorio que se destaca por la belleza y diversidad de los paisajes del agua generados por la presencia de grandes lagos, ríos, fiordos, humedales y campos de hielo. El primer caso de estudio es una investigación para evaluar las potencialidades turísticas y territoriales de los paisajes del agua en la cuenca del río Baker. Se utiliza una metodología interdisciplinaria y técnicas de ...

  5. La construcción discursiva de los imaginarios sociales: el caso de la medicina popular chilena

    Gerardo Álvarez; Andrea Álvarez; Marisol Facuse

    2002-01-01

    En este artículo se da cuenta del estado de avance de una investigación interdisciplinaria (lingüística, psicología, sociología) sobre las representaciones de cuerpo (salud/enfermedad) en la medicina popular chilena (Proyecto FONDECYT 1000376). No se trata de una investigación sobre la medicina como tal. El objetivo es rastrear el imaginario sobre el cuerpo que subyace a las prácticas curativas y que se revela a través del discurso de las sanadoras populares (mapuches y no mapuches), y luego ...

  6. KURSUS SINGKAT DAN PELATIHAN PEMANFAATAN PEPAYA (CARISA PAPAYA L. MENJADI MANISAN KERING BUAH PEPAYA DI DESA TIMUHUN, KECAMATAN BANJARANGKAN, KABUPATEN KLUNGKUNG

    I Made Nada

    2012-09-01

    Full Text Available ABSTRACT In Desa Timuhun, eventhough papaya is not primary fruit product, but usually papaya is planted among the other plants and easily to grow. Papaya has good potency to be processed to become food product. Matured papaya fruit is usually fresh served for desk fruit. On the other hand, for papaya which is not mature yet, usually used for vegetable. Papaya has high water content, so it makes papaya fruit easy to experience damage. With papaya processing, papaya which is originally assumed improper to be sold or to be consumed (mechanical damage, diseased, acid, physical damage and will only to be thrown, finally can be used. The making of papaya dry candy is one of effort to preserve and to vary form of papaya serving. It also improves the economic value of papaya. The making of papaya can be conducted in big or small scale. The activity objectives are to introduce and give additional knowledge to society about papaya processing technology, to increase economic added value of papaya and the using of papaya which is improper to be sold or to be consumed becoming valuable food product .Realization of the objectives above are by performing an information sharing and discussion with the society about process of the making dry candy from papaya, training of papaya dry candy making, introduce simple technology of dry candy papaya making. This activity was performed at the head of Desa Timuhun office, on Friday, September 14, 2007 and was attended by 26 persons, including association of woman farmer and the staff in that office. The result of this activity shows that there was positive response of people who attend this activity, because by processing papaya fruit into dry candy, have a lot of advantage compare to sale it freshly. Beside that, this dry candy is healthy snack, without synthetic preservation, enriched with vitamins which is useful for health, also this dry candy is still good up to 1 month and of course it could to improve economic

  7. Carbon disulfide formation in papaya under conditions of dithiocarbamate residue analysis.

    Abakerli, R B; Sparrapan, R; Sawaya, A C H F; Eberlin, M N; Jara, J L P; Rodrigues, N R; Fay, E F; Luiz, A J B; Galvão, T D L; Martins, D dos S; Yamanishi, O K; Toledo, H H B

    2015-12-01

    Golden, Sunrise Solo and Tainung cultivars of papaya were found to release CS2 when submitted to experimental conditions of dithiocarbamate residue analysis. Three common analytical methods were used to quantitate CS2; one spectrophotometric method and two chromatographic methods. All three methods gave positive CS2 results for all three papaya varieties. Other endogenous compounds present in isooctane extracts of papaya fractions detected via gas chromatography (GC/ITD) using electron ionization (EI) were: carbonyl sulfide, dimethyl sulfide, carbon disulfide, 2-methylthiophene, 3-methylthiophene, 2-ethylthiophene, 3-ethylthiophene, benzylisothiocyanate, benzylthiocyanate and benzonitrile. Control samples were obtained from papaya plantations cultivated in experimental areas, in which no treatment with fungicides of the dithiocarbamate group was applied. Endogenous CS2 levels were compared with true dithiocarbamate residues measured in papaya samples from the field trials following applications of the mancozeb fungicide. Three days after application, true dithiocarbamate residues, measured by the procedure with isooctane partitioning and GC-ITD, were at the average level of 2 mg kg(-1). PMID:26041166

  8. Eradication of Bactrocera papayae (Diptera: Tephritidae) by male annihilation and protein baiting in Queensland, Australia

    An established population of Bactrocera papayae Drew and Hancock (Asian papaya fruit fly) was detected on the Australian mainland near Cairns, north Queensland, on 16 October 1995 (Fay et al. 1997). Coincidentally, the first flies were bred from green papaya, a host not normally utilised in the unripe state by indigenous fruit fly species. B. papayae is a member of the Oriental fruit fly (Bactrocera dorsalis) complex (Drew and Hancock 1994) and has more than 190 known hosts in Southeast Asia, where it is a major pest species. Consequently, the threat to Australian horticulture posed by the incursion was recognised immediately. By 26 October 1995, breeding populations were confirmed around Cairns, Mossman and Mareeba and a Pest Quarantine Area (PQA) was legislated. By 1 November 1995, following detections south of Innisfail, the PQA boundaries were set at 144 deg. 15'E, 19 deg. 00'S, covering nearly 78,000 km2. B. papayae was ultimately trapped over some 20,000 km2. A national eradication campaign began in November 1995, undertaken by the Queensland Department of Primary Industries

  9. Control Strategies of Papaya Mealybug, Paracoccus marginatus Williams and Willink in the Laboratory Condition

    M.J.H. Biswas

    2015-12-01

    Full Text Available The laboratory experiment was conducted at the laboratory of the Department of Entomology, Bangladesh Agricultural University to determine the effectiveness of polythene band, predatory ladybird beetles (available species found in Bangladesh viz. Coccinella transversalis (F., Micraspis discolor (F., Menochilus sexmaculatus (F., Cyclonida sanguinea, Adalia bipunctata, Coccinella novemnotata and Illeis koebelei, four chemical insecticides and three botanical oils in controlling papaya mealybug, Paracoccus marginatus Williams and Granara de Willink. The chemical insecticides were Sevin 85 SP, Dimethoate 40 EC, Fipronil 50 EC, Deltamethrin 2.5 EC, and the botanical oils were Neem oil, Mahogany oil and Karanja oil. In the laboratory experiment, polythene banding failed to prevent the plant from the infestation of papaya mealybug. Seven commonly available species of ladybird beetles in Bangladesh did not provide any control to papaya mealybug. Among the chemical insecticides, Sevin 85 SP and Dimethoate 40 EC showed significant mortality of papaya mealybug. Deltamethrin 2.5 EC and Neem oil (2% provided moderate control of the pest. The remaining insecticides and botanical oils were less effective to control papaya mealybug.

  10. Anglicismos y aculturación en la sociedad chilena (English loanwords: acculturation in chilean society

    Constanza Gerding Salas

    2012-06-01

    Full Text Available La implementación del modelo económico neoliberal estadounidense enChile hace cuatro décadas y la participación del país en la globalización han provocado cambios fundamentales en la sociedad. De hecho, los efectos de la adopción de dicho paradigma se reflejan en diversos ámbitos, incluido el del uso de la lengua. Por esta razón, resulta de interés para lingüistas y traductores indagar acerca de la influencia del idioma inglés y de la cultura estadounidense en la sociedad chilena. De allí que los objetivos de este estudio fueron constatar la presencia de préstamos del inglés en la prensa escrita, para describirlos, determinar su frecuencia de uso e inferir las causas de su adopción. Para lograr estos objetivos se clasificaron de forma manual y semiautomática préstamos del inglés extraídos de periódicos entre enero de 2003 y diciembre de 2009. Se analizó las unidades recopiladas según sus características formales, frecuencia de aparición y áreas del saber a las que pertenecían. Se determinó que los préstamos del inglés se adoptan más que adaptan y que tienen una fuerte presencia en la redacción periodística actual, específicamente en economía, computación, deportes y cultura. Además, se pudo establecer que su uso estaría determinado por cuatro factores: vacío denominativo, prestigio social, economía lingüística y preferencia de los usuarios. Finalmente, se concluyó que la lengua inglesa y la cultura estadounidense tienen una presencia significativa en la prensa chilena. (The implementation of the US-inspired neoliberal economic model in Chile four decades ago and the country’s participation in the globalization process have led to fundamental societal changes. In fact, the effect of the adoption of this paradigm is reflected in several areas, including that of the use of the language. For this reason, it is interesting for linguists and translators to find out the influence of the English language and