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Sample records for papaya chilena vasconcellea

  1. Modelado de la Cinética de Secado de la Papaya Chilena (Vasconcellea pubescens) / Modeling of Drying Kinetic of Chilean Papaya (Vasconcellea pubescens)

    Scientific Electronic Library Online (English)

    Antonio A, Vega; Roberto A, Lemus.

    Full Text Available Se ha estudiado y modelado la cinética de secado por aire caliente de papaya chilena (Vasconcellea pubescens) a diferentes temperaturas (40, 50, 60, 70 y 80º C) con velocidad de aire de 2.0 ± 0.2 m·s-1. Durante las experiencias se observaron los periodos de inducción, velocidad constante y decrecien [...] te. Los modelos matemáticos aplicados fueron el modelo de Newton, Henderson-Pabis y Page. Además se evaluó la calidad de ajuste de estos modelos por medio del coeficiente de regresión lineal, suma de errores cuadrados, raíz media de los errores cuadrados y Chi-cuadrado. Los parámetros cinéticos de cada modelo presentaron la dependencia usual con la temperatura, y fueron evaluadas con la ecuación de Arrhenius. Al comparar los valores experimentales con los calculados, se demostró que el modelo de Page obtuvo la mejor calidad de ajuste en cada curva de secado, representando una excelente herramienta para estimar el tiempo de secado de este producto. Abstract in english The kinetics of hot air drying of chilean papaya (Vasconcellea pubescens) was studied and modeled at different temperatures (40, 50, 60, 70 & 80º C) and air velocity of 2.0 ± 0.2 m·s-1. The induction, constant, and fallingrate periods were observed during the experimentation. The mathematical models [...] of Newton, Henderson-Pabis and Page were applied. Also evaluated were the quality of fit to the models using the coefficient of linear regression, the sum square error, the root mean square error and the Chisquare test. The kinetic parameters of each model showed the usual dependence on temperature, and were evaluated using the Arrhenius equation. In comparing experimental data with calculated values, it was demonstrated that the Page model attained the best fit for every drying curve, representing an excellent tool for the estimation of the drying time of this product.

  2. Obtención, regeneración y evaluación de híbridos intergenéricos entre Carica papaya Y Vasconcellea cauliflora

    Scientific Electronic Library Online (English)

    Ariadne, Vegas; Gustavo, Trujillo; Yanet, Sandrea.

    2003-12-01

    Full Text Available O resgate de híbridos provenientes de cruzamentos intergenéricos entre mamão (Carica papaya L.) e outras espécies de vasconcelleas (antes conhecidas como caricas) possibilita a tranferência de genes das espécies selvagens para a cultivada. O objectivo do presente trabalho foi a obtenção e avaliação [...] de plantas híbridas mediante o resgate e regeneração de embriões ou óvulos resultantes do cruzamento entre C. papaya e Vasconcellea cauliflora. Dos cruzamentos efectuados, 0 a 76% formaram frutos, de acordo com o tipo de papaya usado. As sementes presentes nos frutos foram vás, inmaduras o possuiam embriões zigóticos. Na maioria dessas sementes ocorreu poliembrionia zigótica in vivo, embora também foram formado alguns híbridos individuais. Conseguiu-se o desenvolvimento, germinação, multiplicação de embriões híbridos e regeneração de plantas in vitro. As plantas establecidas em campo produziram flores andróicas y andromonóicas. Abstract in spanish El rescate de híbridos de cruces intergenéricos entre la lechosa (Carica papaya L.) y otras especies de vasconcelleas (antes conocidas como caricas) posibilita la transferencia de genes desde las especies silvestres hacia la cultivada. El objetivo del presente trabajo fue la obtención y evaluación d [...] e plantas híbridas mediante el rescate y regeneración de embriones u óvulos después del cruce entre C. papaya y Vasconcellea cauliflora. Se obtuvo un porcentaje de fructificación entre 0 y 76%, de acuerdo al tipo de lechosa usada en el cruce. En los frutos, las semillas fueron vanas, inmaduras o contenían embriones cigóticos. En la mayoría de ellas ocurrió la poliembrionía cigótica in vivo, aún cuando también se produjeron híbridos individuales. Se logró el desarrollo, germinación y multiplicación de embriones híbridos, y la regeneración de plantas in vitro. Las plantas sembradas en campo produjeron flores andróicas y andromonóicas. Abstract in english The rescue of hybrids from intergeneric crosses between papaya (Carica papaya L.) and other vasconcelleas species (known before as caricas) can make possible gene transfer from a wild species to a cultivated one. The object of this research was to obtain and evaluate hybrid plants after rescue and r [...] egeneration of embryos or ovules, from the crosses made between C. papaya and Vasconcellea cauliflora. After cross pollination, 0 to 76% fructification was attained, according to the papaya type used. In the fruits, seeds were vain, immature or contained zygotic embryos. In most of them occurred in vivo zygotic polyembryony, although some individual hybrids were also formed. Development, germination, multiplication of embryo hybrids and plant regeneration in vitro were achieved. In the field, plants produced androic and andromonoic flowers.

  3. Susceptibilidad de Vasconcellea cauliflora al virus de la mancha anillada de la lechosa / Susceptibility of Vasconcellea cauliflora to Papaya ringspot virus

    Scientific Electronic Library Online (English)

    A, Gonzalez; G, Trujillo.

    2005-07-01

    Full Text Available Esta investigación demostró la susceptibilidad de Vasconcellea cauliflora, especie que ha sido considerada como resistente al virus de la mancha anillada de la lechosa (PRSV-P). La transmisión de la enfermedad se logró mediante la inoculación mecánica de una cepa severa del PRSV-P en plantas de 60 d [...] ías de edad. Estas evidenciaron síntomas de necrosis del cogollo en 8,3% bajo condiciones de invernadero a 25,15ºC y 78,68% HR promedio. Bajo condiciones de campo en la Facultad de Agronomía (UCV), Maracay (440 msnm), se contabilizó el 8,6% de plantas inoculadas con síntomas típicos de la enfermedad a los siete días del trasplante. Posteriormente se realizó un ensayo bajo condiciones controladas en cámara de crecimiento. Las plantas inoculadas fueron expuestas a tres tratamientos de temperatura e intensidad lumínica: T1= 29ºC y 6300 lux; T2= 27ºC y 5500 lux y T3= 25ºC y 4200 lux; con fotoperiodo de 16 horas de luz y 75,5% HR promedio. Los resultados demostraron la transmisión del PRSV-P en 82, 86 y 47% respectivamente; la sintomatología viral se corroboró mediante el uso de ELISA. Estos resultados cuestionan el uso de esta especie como fuente de genes de resistencia al PRSV-P en los programas de mejoramiento de lechosa. Abstract in english This work demonstrated the susceptibility of Vasconcellea cauliflora, a species considered as virus resistant to Papaya ringspot virus (PRSV-P). Disease transmission by mechanical inoculation of PRSV-P severe strain on 60 days old plants was corroborated. Under greenhouse conditions with average val [...] ues of 25.15ºC and 78.68% RH, 8.3% of plants inoculated with the severe strain showed apical necrosis. At the Facultad de Agronomía (UCV), Maracay (440 msnm) under field conditions, 8.6% of inoculated plants showed typical symptoms of the disease seven days after beings transplanted. Subsequently, an inoculation test was done in three growth chamber conditions: T1=29ºC and 6300 lux; T2= 27ºC and 5500 lux and T3= 25ºC and 4200 lux; the daily time was fixed at 16 h, and the relative humidity was about 75.5% on average. Systemic symptoms were observed in 82, 86 and 47% of plants in treatments T1, T2 y T3 respectively. These results were consistent with the ELISA test. The susceptibility of V. cauliflora to the local severe strain of PRSV-P was influenced by temperature and light intensity conditions. This questions the role of this species in search for PRSV-P resistance genes in papaya breeding programs.

  4. Susceptibilidad de Vasconcellea cauliflora al virus de la mancha anillada de la lechosa Susceptibility of Vasconcellea cauliflora to Papaya ringspot virus

    Directory of Open Access Journals (Sweden)

    A Gonzalez

    2005-07-01

    Full Text Available Esta investigación demostró la susceptibilidad de Vasconcellea cauliflora, especie que ha sido considerada como resistente al virus de la mancha anillada de la lechosa (PRSV-P. La transmisión de la enfermedad se logró mediante la inoculación mecánica de una cepa severa del PRSV-P en plantas de 60 días de edad. Estas evidenciaron síntomas de necrosis del cogollo en 8,3% bajo condiciones de invernadero a 25,15ºC y 78,68% HR promedio. Bajo condiciones de campo en la Facultad de Agronomía (UCV, Maracay (440 msnm, se contabilizó el 8,6% de plantas inoculadas con síntomas típicos de la enfermedad a los siete días del trasplante. Posteriormente se realizó un ensayo bajo condiciones controladas en cámara de crecimiento. Las plantas inoculadas fueron expuestas a tres tratamientos de temperatura e intensidad lumínica: T1= 29ºC y 6300 lux; T2= 27ºC y 5500 lux y T3= 25ºC y 4200 lux; con fotoperiodo de 16 horas de luz y 75,5% HR promedio. Los resultados demostraron la transmisión del PRSV-P en 82, 86 y 47% respectivamente; la sintomatología viral se corroboró mediante el uso de ELISA. Estos resultados cuestionan el uso de esta especie como fuente de genes de resistencia al PRSV-P en los programas de mejoramiento de lechosa.This work demonstrated the susceptibility of Vasconcellea cauliflora, a species considered as virus resistant to Papaya ringspot virus (PRSV-P. Disease transmission by mechanical inoculation of PRSV-P severe strain on 60 days old plants was corroborated. Under greenhouse conditions with average values of 25.15ºC and 78.68% RH, 8.3% of plants inoculated with the severe strain showed apical necrosis. At the Facultad de Agronomía (UCV, Maracay (440 msnm under field conditions, 8.6% of inoculated plants showed typical symptoms of the disease seven days after beings transplanted. Subsequently, an inoculation test was done in three growth chamber conditions: T1=29ºC and 6300 lux; T2= 27ºC and 5500 lux and T3= 25ºC and 4200 lux; the daily time was fixed at 16 h, and the relative humidity was about 75.5% on average. Systemic symptoms were observed in 82, 86 and 47% of plants in treatments T1, T2 y T3 respectively. These results were consistent with the ELISA test. The susceptibility of V. cauliflora to the local severe strain of PRSV-P was influenced by temperature and light intensity conditions. This questions the role of this species in search for PRSV-P resistance genes in papaya breeding programs.

  5. COMPORTAMIENTO DE MATERIALES DE LOS GÉNEROS Carica Y Vasconcellea FRENTE A Erwinia papayae, Meloidogyne incognita Y Rotylenchulus reniformis / REACTION OF THE GENERA Carica AND Vasconcellea MATERIALS TO Erwinia papayae, Meloidogyne incognita AND Rotylenchulus reniformis

    Scientific Electronic Library Online (English)

    Anna, Maselli; Ligia Carolina, Rosales; Yolanda, Guevara; Zoraida, Suárez H.

    2010-12-01

    Full Text Available La bacteriosis del cancro y el ataque de los nematodos constituyen graves obstáculos en la extensión del cultivo de la papaya (Carica papaya L.). El objetivo principal de este trabajo fue la selección de genotipos tanto comerciales como silvestres resistentes a los patógenos mencionados. Para la eva [...] luación del comportamiento ante la bacteria del cancro causada por Erwinia papayae, se seleccionaron once accesiones de C. papaya, Vasconcellea goudotiana y V. cauliflora y se inocularon 10 plantas de cada genotipo con una suspensión bacteriana de concentración de 10(8) UFC.mL-1. La inoculación se realizó produciendo heridas en los tallos de las plantas sanas. Los testigos se trataron de igual forma con agua destilada estéril. Para la evaluación de la resistencia a nematodos se realizaron dos experimentos; en el primero se utilizaron materiales de C. papaya y V. goudotiana, los cuales se inocularon con una población mixta de Meloidogyne incognita raza 1 y Rotylenchulus reniformis con 2000 huevos+juveniles.1000 cm-3 en suelo-arena estéril. Para el segundo experimento se usaron, además de los materiales mencionados, V. cauliflora, V. cundinamarcensis, V. microcarpa var. microcarpa, V. microcarpa var. pilifera, inoculados sólo con M. incognita raza 1 con 2500 huevos+juveniles.1000 cm-3. A las 12 semanas se calculó la población final y se midieron las siguientes variables: peso aéreo y radical fresco y seco. Los resultados del comportamiento de los genotipos frente a la bacteria determinó que V. goudotiana y V. cauliflora no se enfermaron, no así el género Carica, donde todas las plantas resultaron susceptibles, coincidiendo con la evaluación de nematodos, donde todas las accesiones de C. papaya y V. goudotiana fueron susceptibles y no tolerante al ataque de la población mixta de M. incognita raza 1 y R. reniformis, por afectarse las variables agronómicas evaluadas; mientras que la mayoría de los materiales de V. cundinamarcencis y V. microcarpa resultaron resistentes al ataque de M. incognita raza 1. Estos resultados permiten la obtención de genes de resistencias en los materiales silvestres, los cuales podrían ser incorporados a los genotipos mejorados o comerciales, y permitiría un mejor manejo de los patógenos mencionados, que resultan limitantes severos en el cultivo de papaya en Venezuela. Abstract in english The bacterial canker and the attack of nematodes are serious obstacles to the extension of Carica papaya L. crop. The main objective of this work was the selection of both commercial and wild genotypes resistant to the above mentioned pathogens. To assess the response to the bacterial canker caused [...] by Erwinia papayae, eleven accesions of C. papaya, Vasconcellea goudotiana y V. cauliflora were selected and ten plants of each genotype were inoculated with a bacterial suspension with a concentration of 10(8) CFU.mL-1 . The plants were inoculated by wounds on the stems of the healthy plants. The controls were similarly treated but with sterile distilled water. Two trials were carried out to evaluate the resistance to nematodes. In the first trial, materials of C. papaya and V. goudotiana were inoculated with a mixed population of Meloidogyne incognita race 1 and Rotylenchulus reniformis, with 2000 eggs+juveniles. 1000 cm-3 in sterile soil-sand. In the second trial, V. cauliflora, V. cundinamarcensis, V. microcarpa var. microcarpa and V. microcarpa var. pilifera, in addition to the genotypes previously used, were inoculated only with M. incognita race 1, with 2500 eggs+juvenils.1000 cm-3. After 12 weeks, the final population was calculated and the fresh and dried weights of the aerial parts and roots were determined. The results of the reaction of the genotypes towards the bacterium determined that V. goudotiana and V. cauliflora did not get the disease, what differed from the Carica genus, where all the plants resulted susceptible, a result that was similar to the evaluation to nematodes, where all the genotypes of C. papaya and V. goudotiana were susceptible and

  6. Estudios de germinación y remoción de latencia en semillas de papayuelas Vasconcellea cundinamarcensis y Vasconcellea goudotiana / Highland papayas Vasconcellea cundinamarcensis and Vasconcellea goudotiana seed germination and dormancy release studies

    Scientific Electronic Library Online (English)

    Sandra Patricia, Benítez; Lobo, Mario; Oscar Arturo, Delgado; Clara Inés, Medina.

    2013-12-01

    Full Text Available Se estudió la germinación y latencia de las semillas de Vasconcellea cundinamarcensis y V. goudotiana, para desarrollar procedimientos promotores de emergencia de los embriones. La categorización de las semillas viables no germinables se realizó con el empleo del tetrazolio. La fermentación prolonga [...] da de las semillas, para remover la sarcotesta, afectó la viabilidad de éstas, en especial las de V. cundinamarcensis. Aplicaciones de KNO3 y AG3 promovieron una germinación significativamente mayor, en comparación con el testigo, en semillas fermentadas previamente durante 15 días de V. goudotiana, lo cual no ocurrió en las de V. cundinamarcensis. La aplicación de Saccharomyces cerevisiae durante 36 horas, para fermentación y liberación de la sarcotesta, indujo germinación en las dos especies, con una respuesta significativamente superior en V. goudotiana. La adición de AG3 y KNO3, luego del tratamiento anterior, promovió mayor emergencia en ambos taxa, en especial en V. goudotiana. La fermentación de las semillas con Saccharomyces cerevisiae, en combinación con AG3 y KNO3 produjo germinación en las dos especies, con énfasis en V. goudotiana al aplicar 1000 ppm de AG3 + KNO3 al 2,5% y en V. cundinamarcensis, al utilizar 600 ppm de AG3 + AG3 al 2,5%, sin diferencia significativa con la aplicación de 1000 ppm de AG3 + KNO3 al 1,5%; se logró mayor germinación en V. goudotiana en el tratamiento precitado, con el cual se obtuvo con confiabilidad estadística la máxima brotación de plántulas, en relación con el resto de tratamientos evaluados con la propia especie y en V. cundinamarcensis. Abstract in english Seed germination and dormancy of Vasconcellea cundinamarcensis and V. goudotiana were studied in order to develop procedures for seed embryo emergency. Characterization of viable dormant seeds was done with the tetrazolium test. Long periods of seed fermentation, for sarcotestal removal, affected th [...] e seed viability, mainly in V. cundinamarcensis. AG3 and KNO3 application promoted a significantly higher germination, in V. goudotiana, but not in V. cundinamarcensis, in relation to control seeds, fermented during 15 days. AG3 and KNO3 application promoted a significantly higher germination in V. goudotiana, but not in V. cundinamarcensis, in relation to control 15 days fermented seeds. Seed fermentation with Saccharomyces cerevisiae during 36 hours for sarcotest removal, promoted a significant higher germination than that of the control, fermented without enzyme application, mainly in V. goudotiana. Seed fermentation with S. cerevisiae during 36 hours application, for sarcotest removal, promoted a significant higher germination than that of the control, fermented without enzyme application, mainly in V. goudotiana. Seed germination, after S. cerevisiae fermentation and application of AG3 and KNO3, occurred in both species, being obtained the best germination in V. goudotiana, with application of 1000 ppm of GA3 + 2.5% KNO3, with statistical differences, related to the other germination applied treatments. The best germination results, without significant differences, in V. cundinamarcensis were obtained with the application of 600 ppm of GA3 + 2.5% KNO3 or 1000 ppm of GA3 + 1.5% KNO3, after the fermentation procedure with S. cerevisiae.

  7. Palynology of Carica and Vasconcellea (Caricaceae Palinología de Carica y Vasconcellea (Caricaceae

    Directory of Open Access Journals (Sweden)

    Lagos Túlio César

    2006-09-01

    Full Text Available

    Palynology of Carica and Vasconcellea (Caricaceae. The pollen of C. papaya and agreements of Vasconcellea cauliflora, V. cundinamarcensis, V. crassipetala, V. goudotiana, V. x heilbornii var. chrysopetala, V. longiflora and V. sphaerocarpa collected in the Colombian Coffee Growing Zone, using the technique of acetolisis for optic microscopy and the fixation procedure with glutaraldehide, dehydration and ionization with gold-palade, for scanning electronic microscopy was described. The pollen grains were characterized using descriptors, which include the characters of taxonomic value for pollen identification, genetically determined. The most important are the number, position and character of the aperture (NPC and exine ornamentation and stratification. The pollen is of medium size for both genera, tricolporate, zonoaperturate, prolate-spheroid to subprolate, isopolar radial symmetry, tectate, dug, foveolate, with columelas. These characters have demonstrated a great contribution to the taxonomy of Caricaceae because the cluster analysis allowed distinguish very well the two genera.

    Se describe el polen de C. papaya y accesiones de Vasconcellea cauliflora, V. cundinamarcensis, V. crassipetala, V. goudotiana, V. x heilbornii var. chrysopetala, V. longiflora y V. sphaerocarpa recolectadas en la Zona Cafetera de Colombia, empleando la técnica de acetólisis para microscopía óptica y el procedimiento de fijación con glutaraldehído, deshidratación e ionización con oro paladio, para microscopía electrónica de barrido (MEB. Se caracterizaron los granos de polen por medio de una lista de descriptores que incluyeron los caracteres de valor taxonómico para identificación del polen, genéticamente determinados, siendo los más importantes el número, posición y carácter de la abertura (NPC y la ornamentación y estratificación de la exina. Para los dos géneros el polen es mediano, tricolporado, zonoaperturado, prolato–esferoidal a subprolato, de simetría radial isopolar, tectado, cavado, foveolado, con columelas. Estos caracteres han demostrado gran aporte a la taxonomía de Caricaceae, ya que el análisis de agrupamiento permitió diferenciar bien a los dos géneros.

  8. Citogenética de especies de Vasconcellea (Caricaceae

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    Caetano Creucí María

    2008-12-01

    Full Text Available Conocer aspectos básicos de la citogenética de las papayas de montaña o papayuelas, especies del género Vasconcellea (Caricaceae originarias de Ecuador y Colombia, fue el objetivo del trabajo. Botones florales se fijaron en etanol-acético 3:1 por 24h, luego se transfirieron a nueva solución y se almacenaron en frío. Los microsporocitos y los granos de polen se tiñieron con acetocarmín (1%. Se evaluaron las fases de meiosis y la viabilidad de polen para describir el comportamiento cromosómico. Se encontró que: 1 todas las seis especies estudiadas fueron diploides (2n=2x=18; 2 se describió el número cromosómico para V. sphaerocarpa, V. longiflora y V. palandensis (2n=18; 3 el grado de asimetría de los complementos cromosómicos indicó un proceso de evolución; 4 la presencia de numerosos NOR en V. sphaerocarpa está relacionada con los micronucleolos y corroboró el posible origen híbrido; y 5 V. cauliflora (42.98% y V. cundinamarcensis (47.93% presentaron la más baja viabilidad polínica.

  9. Intestinal obstruction due to Vasconcellea seeds: Report of three cases and literature review

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    Montoya-González, Juliana

    2015-07-01

    Full Text Available Vasconcellea spp., is a species of Andean papaya commonly cultivated in rural communities close to Medellín, Colombia. Due to the pleasant and sweet flavor of its fruits, children frequently ingest its seeds accidentally. After ingestion, the seeds are engaged in the colonic lumen and block the exit of stools, causing pain and bloating, and promoting bacterial translocation. Diagnosis is based on clinical history and rectal examination. Treatment depends on the degree of local and systemic involvement and includes disimpaction of rectal contents under general anesthesia and colonic washes with 0.9% saline solution. In severe cases derivative colostomy has been required. In the literature there are no reports of intestinal obstruction due to Vasconcellea seeds, possibly because it has been mistaken for seeds of the genus Carica. In this article, three cases treated at pediatric services in Medellín, Colombia, in 2012 and 2013 are described. We note that this is a rarely suspected disease, leading to late diagnosis and potential catastrophic consequences. It is important to educate people to prevent the ingestion of the seeds.

  10. Mejoramiento de la germinación, control de la hiperhidricidad y formación de brotes en Vasconcellea stipulata Badillo

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    Rosa Armijos González

    2015-11-01

    Full Text Available Vasconcellea stipulata has great commercial importance because of its enzymatic activity and as a source for genetic improvement of papaya since it is resistant to the papaya ringspot virus. However, due to its low regeneration by seeds and limited knowledge of its genetic and pharmaceutical properties, this species is not widely cultivated. For propagation, in vitro culture of seeds has been used to address this problem, but hyperhydricity, a physiological disorder, mainly expressed in the developing embryonic axis and specifically associated with this species, is a significant constraint. In order to obtain elite material for culture of V. stipulata, the aim of this work was to increase germination, to control hyperhydricity in embryos and to evaluate the potential to induce morphogenic responses, i.e., shoot formation. Our results showed that it is possible to increase germination up to 53% under in vitro conditions within a short period in the presence of hydrogen peroxide. In addition, hyperhydricity was significantly reduced (50% in vitro when gibberellic acid concentrations were included on a 1/2 Nitsch and Nitsch nutrient medium, resulting in approximately 80% recovery of viable seedlings. Finally, other plant growth regulators were evaluated and found to trigger shoot formation in axillary buds as well as induce the formation of callus in leaf sections derived of seedlings.

  11. NOTE - ISSR markers for genetic relationships in Caricaceae and sex differentiation in papaya

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    Telma Nair Santana Pereira

    2011-01-01

    Full Text Available ISSR markers are polymorphic and their results easily reproducible. They are therefore intensely used in phylogeneticstudies and sex differentiation of some economically interesting plant species. The objectives of this study were to analyze the geneticdiversity in Caricaceae using ISSR markers, to identify a specific ISSR band that could distinguish female from hermaphroditepapaya genotypes and to verify whether this marker could be used for early sex differentiation. The ISSR-PCR was performed withnine primers and they could distinguish all species. It was observed that Jacaratia spinosa was closer to Vasconcellea than toCarica. The species C. papaya was only distantly related to both genera. A 500 bp ISSR marker was found in 25 % of the papayagenotypes studied. Specifically in these cases this marker could be used for early sex differentiation in papaya.

  12. BASKETFUL BENEFITS OF PAPAYA

    OpenAIRE

    Parle Milind; Gurditta

    2011-01-01

    Papaya (Carica papaya linn) is well known for its exceptional nutritional and medicinal properties throughout the world. From the times immemorial, the whole Papaya plant including its leaves, seeds, ripe and unripe fruits and their juice is used as a traditional medicine. The fruit has a large oval shape, yellowish-green skin and yellow flesh. Nowadays, Papaya is considered as a Nutraceutical fruit due to its multi-faceted medicinal properties. The prominent medicinal properties of Papaya in...

  13. ISSR markers for genetic relationships in Caricaceae and sex differentiation in papaya / Marcadores ISSR nas relações genéticas em Cariaceae e na identificação sexual do mamoeiro

    Scientific Electronic Library Online (English)

    Fabiane Rabelo da, Costa; Telma Nair Santana, Pereira; Ana Paula Candido, Gabriel; Messias Gonzaga, Pereira.

    2011-12-01

    Full Text Available Os marcadores ISSR apresentam amplo polimorfismo e alta reprodutibilidade de resultados, o que tem intensificado seu uso em estudos filogenéticos e na diferenciação sexual de algumas espécies de interesse econômico. Os objetivos deste trabalho foram analisar a divergência genética em Caricaceae util [...] izando-se marcadores ISSR, identificar uma marca capaz de diferenciar plantas hermafroditas e femininas em mamoeiro e verificar se este marcador pode ser utilizado na sexagem precoce de diferentes genótipos da espécie. O estudo foi conduzido com nove primers, os quais foram capazes de distinguir todas as espécies. Observou-se que Jacaratia spinosa ficou mais próxima de Vasconcellea do que de Carica. A espécie C. papaya mostrou-se geneticamente distante de ambos os gêneros. Verificou-se ainda a presença de um fragmento ISSR de 500 pb em 25 % dos genótipos de mamoeiro estudados, podendo ser usado para auxiliar a sexagem precoce do mamoeiro especificamente nesses casos. Abstract in english ISSR markers are polymorphic and their results easily reproducible. They are therefore intensely used in phylogenetic studies and sex differentiation of some economically interesting plant species. The objectives of this study were to analyze the genetic diversity in Caricaceae using ISSR markers, t [...] o identify a specific ISSR band that could distinguish female from hermaphrodite papaya genotypes and to verify whether this marker could be used for early sex differentiation. The ISSR-PCR was performed with nine primers and they could distinguish all species. It was observed that Jacaratia spinosa was closer to Vasconcellea than to Carica. The species C. papaya was only distantly related to both genera. A 500 bp ISSR marker was found in 25 % of the papaya genotypes studied. Specifically in these cases this marker could be used for early sex differentiation in papaya.

  14. Sex Determination in Papaya

    Science.gov (United States)

    Sex determination is an intriguing system in trioecious papaya. Over the past seven decades various hypotheses, based on the knowledge and information available at the time, have been proposed to explain the genetics of the papaya's sex determination. These include a single gene with three alleles...

  15. Características Físico-Químicas del Látex de Papayuelo (Vasconcellea cundinamarcensis Badillo, Caricaceae Physico-Chemical Characteristics of Papayuelo Latex (Vasconcellea Cundinamarcensis Badillo, Caricaceae

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    Leslie V Vidal

    2009-01-01

    Full Text Available Se determinó el rendimiento y la actividad enzimática de la papaína presente en el látex de papayuelo (Vasconcellea cundinamarcensis, cultivado en Cobquecura, VIII región del Bío-Bío, Chile, durante un año. Entre enero y diciembre, las plantas, frutos y látex, obtenidos de frutos inmaduros, fueron caracterizados con el propósito de conocer el comportamiento del cultivo a través de las estaciones del año, y comprender las posibles relaciones entre los parámetros estudiados. El diseño experimental fue completamente aleatorio y los datos se procesaron mediante un ANDEVA y prueba de rangos múltiples de Duncan. Se encontró diferencia estadística significativa entre estaciones en cuanto al desarrollo de la planta y sus características. Los mayores rendimientos de látex se obtuvieron en la estación de primavera, mientras que la actividad enzimática se mantuvo constante durante el año de estudio.A study to determine the yield and enzymatic activity of papain present in the latex obtained from papayuelo fruit (Vasconcellea cundinamarcensis cultivated in Cobquecura, VIII Bío-Bío Region in Chile, during one year was done. Between January and December the papayuelo plants, fruits and latex obtained from unripe fruits were characterized. The objective of the study was to know the species behavior through the seasons and the relation between the parameters studied. The experimental design was completely random, and the data were processed through ANOVA and Duncan’s multiple range test. A significant statistical difference in plant growth and plan characteristics between seasons was found. The higher latex yields were obtained in spring, whereas the enzymatic activity was constant through the year.

  16. Características Físico-Químicas del Látex de Papayuelo (Vasconcellea cundinamarcensis Badillo, Caricaceae) / Physico-Chemical Characteristics of Papayuelo Latex (Vasconcellea Cundinamarcensis Badillo, Caricaceae)

    Scientific Electronic Library Online (English)

    Leslie V, Vidal; Víctor L, Finot; Karina del C, Mora; Fernando A, Venegas.

    Full Text Available Se determinó el rendimiento y la actividad enzimática de la papaína presente en el látex de papayuelo (Vasconcellea cundinamarcensis), cultivado en Cobquecura, VIII región del Bío-Bío, Chile, durante un año. Entre enero y diciembre, las plantas, frutos y látex, obtenidos de frutos inmaduros, fueron [...] caracterizados con el propósito de conocer el comportamiento del cultivo a través de las estaciones del año, y comprender las posibles relaciones entre los parámetros estudiados. El diseño experimental fue completamente aleatorio y los datos se procesaron mediante un ANDEVA y prueba de rangos múltiples de Duncan. Se encontró diferencia estadística significativa entre estaciones en cuanto al desarrollo de la planta y sus características. Los mayores rendimientos de látex se obtuvieron en la estación de primavera, mientras que la actividad enzimática se mantuvo constante durante el año de estudio. Abstract in english A study to determine the yield and enzymatic activity of papain present in the latex obtained from papayuelo fruit (Vasconcellea cundinamarcensis ) cultivated in Cobquecura, VIII Bío-Bío Region in Chile, during one year was done. Between January and December the papayuelo plants, fruits and latex ob [...] tained from unripe fruits were characterized. The objective of the study was to know the species behavior through the seasons and the relation between the parameters studied. The experimental design was completely random, and the data were processed through ANOVA and Duncan’s multiple range test. A significant statistical difference in plant growth and plan characteristics between seasons was found. The higher latex yields were obtained in spring, whereas the enzymatic activity was constant through the year.

  17. BASKETFUL BENEFITS OF PAPAYA

    Directory of Open Access Journals (Sweden)

    Parle Milind

    2011-07-01

    Full Text Available Papaya (Carica papaya linn is well known for its exceptional nutritional and medicinal properties throughout the world. From the times immemorial, the whole Papaya plant including its leaves, seeds, ripe and unripe fruits and their juice is used as a traditional medicine. The fruit has a large oval shape, yellowish-green skin and yellow flesh. Nowadays, Papaya is considered as a Nutraceutical fruit due to its multi-faceted medicinal properties. The prominent medicinal properties of Papaya include Anti-fertility, Uterotonic, Diuretic, Anti-hypertensive, Hypolipidemic, Anti-helmintic, Wound-healing, Anti-fungal, Anti-bacterial, Anti-tumor and Free radical scavenging activities. Phytochemically, the whole plant contains enzymes (Papain, carotenoids, alkaloids, monoterpenoids, flavonoids, minerals and vitamins. In the present review article, a humble attempt is made to compile all the strange facts available about this tasty fruit. This tasty fruit of Papaya is popular among family members of all ages for the delicious dishes derived from it.

  18. Papaya: environment and crop physiology

    Science.gov (United States)

    Papaya (Carica papaya L.) is a principal horticultural crop of tropical and subtropical regions. Knowledge of how papaya responds to environmental factors provides a scientific basis for the development of management strategies to optimize fruit yield and quality. A better understanding of genotyp...

  19. In vitro morphogenic responses of Vasconcellea chilensis Planch. ex A. DC (Caricaceae

    Directory of Open Access Journals (Sweden)

    Jordan Z. Miguel

    2011-12-01

    Full Text Available

    Multiple shoot formation was induced in vitro on nodal sections excised from adult “palo gordo” plants of Vasconcellea chilensis ex Carica chilensis, in the presence of relatively high levels of thidiazuron (TDZ, indole-3- acetic acid (IAA and organic addenda included in the WPM formulation including casein hydrolysate, adenine sulfate and cystein. Multiple shoots appeared simultaneously in strands or clusters on the surface of the explants; usually two or more clusters with new shoots were initiated, all showing synchronous growth. In subculture, in the presence of indole-3-butyric acid (IBA as single hormone, root formation did not take place; the shoots turned green instead, evidenced elongation and development of new shoots at the base of the explants. Other explants, such as petioles and leaf sections exhibited callus initiation upon the vascular system after 4-5 weeks; however, this tissue turned brown later on and died.

  20. en la adolescencia chilena

    Directory of Open Access Journals (Sweden)

    Luis Cárcamo Ulloa

    2008-01-01

    Full Text Available En medio de los cambios que generado la llamada Era de la Información, adquiere importancia el fenómeno de la Comunicación Mediada por Computador (CMC. Esto se relaciona con el creciente acceso de la sociedad chilena hacia la internet, que lo posiciona como líder en Latinoamérica en el tema. Dada la cercanía que los jóvenes tienen con las nuevas tecnologías, este grupo etáreo es el que se apropia con mayor intensidad de esta nueva herramienta comunicativa. La mensajería instantánea pasa a ser una importante herramienta para los procesos de socialización de los jóvenes, ocupando el lugar que en décadas pasadas tuvo el teléfono. A diferencia de otras tecnologías, la mensajería se convierte en uno de los pocos elementos comunes que cruza los segmentos sociales y las variadas tribus urbanas en las cuales los jóvenes chilenos viven sus procesos de construcción de la identidad.

  1. IN VITRO RHIZOGENESIS IN PAPAYA (CARICA PAPAYA L.

    Directory of Open Access Journals (Sweden)

    Jaime A. TEIXEIRA DA SILVA

    2013-12-01

    Full Text Available The seeds of two papaya (Carica papaya L. cultivars ('Rainbow' and 'Sunrise Solo' were germinated on Murashige and Skoog (MS medium with 3% sucrose, and free of plant growth regulators. Papaya contains some important secondary metabolites such as papain, and there would be interest in the in vitro mass production of papaya tissue of uniform origin. The most obvious form would be through the induction of somatic embryos, but rhizogenesis, an unexplored method, could provide as-yet unknown advantages. In this study, with the objective of artificaially inducing rhizogenesis in vitro, young leaves of both cultivars were placed on MS basal medium exposed to 5 concentrations (0, 1, 2, 4 or 8 mg/l of auxins (2,4,5-trichlorophenoxyacetic acid, 2,4,5-T; indole-3-acetic acid, IAA; indole-3-butyric acid, IBA; ?-naphthaleneacetic acid, NAA; ?-naphthoxyacetic acid, BNOA or phloroglucinol. All auxins could induce adventitious roots. Most roots (23/explant formed with 2 mg/l NAA. The ability to induce only roots without any other intermediary organs such as callus or shoots provides an exclusive system for possible root-specific secondary metabolite production without the need for transgenic technologies such as Agrobacterium rhizogenes, or could provide a model protocol for more in-depth developmental studies on root development in papaya, an unexplored topic for this tropical plant.

  2. Digital Transcriptome Analysis of Putative Sex-Determination Genes in Papaya (Carica papaya)

    OpenAIRE

    Urasaki, Naoya; Tarora, Kazuhiko; Shudo, Ayano; Ueno, Hiroki; Tamaki, Moritoshi; Miyagi, Norimichi; Adaniya, Shinichi; MATSUMURA, Hideo

    2012-01-01

    Papaya (Carica papaya) is a trioecious plant species that has male, female and hermaphrodite flowers on different plants. The primitive sex chromosomes genetically determine the sex of the papaya. Although draft sequences of the papaya genome are already available, the genes for sex determination have not been identified, likely due to the complicated structure of its sex-chromosome sequences. To identify the candidate genes for sex determination, we conducted a transcriptome analysis of flow...

  3. Ocorrência de Ovulariopsis papayae Bijl em folhas de mamoeiro Occurrence of Ovulariopsis papayae Bijl in papaya leaves

    Directory of Open Access Journals (Sweden)

    Ivan José Antunes Ribeiro

    1988-01-01

    Full Text Available É citada pela primeira vez, em nossas condições, a ocorrência de oídio causado por Ovulariopsis papayae Bijl em folhas de mamoeiro (Cacica papaya L.. São descritas a sintomatologia, as características morfológicas do patógeno e o teste de patogenicidade.The occurrence causing powdery mildew in papaya leaves was observed for the first time in the State of São Paulo, Brazil. The symptomatology and the morphological characteristics of the pathogen were described and the pathogenicity was comproved.

  4. Managing papaya ringspot virus: Impact of grass barriers on alate aphid immigration into papaya orchards

    Science.gov (United States)

    Papaya ringspot virus, transmitted by alate aphids, is the most limiting factor of papaya production in the Caribbean region. Although there are transgenic papaya varieties that provide protection from this virus, these varieties are effective only in certain regions against certain strains of the v...

  5. Physical-chemical analyses of irradiated papayas (Carica papaya L.)

    International Nuclear Information System (INIS)

    Papaya is cultivated in Espirito Santo State/Brazil and as it stands up to irradiation, it is important to validate this technology, since it is already being applied in some countries. Penetration energy, ratio (relation between soluble solids and titrable acidity) and skin color were evaluated to verify the influence of four different doses of irradiation (0.0, 0.5, 0.75 and 1.00 kGy) on papayas, during 21 days. As a result for the skin color and the penetration energy, it was found that in the first days after irradiation, these variables increased with increase in radiation dose; however, after a time lapse, the tendency inverted and the irradiated fruits had a slower ripening process. For the ratio, a very important variable that it is responsible for the fruit taste, no difference was found between irradiated and the control fruit. Color and texture measurements are dependent on the storage temperature

  6. Somatic embryogenesis of Carica Papaya

    International Nuclear Information System (INIS)

    This paper describes the somatic embryogenesis of Carica papaya. Culture medium used was1/2 strength MS basal medium supplemented with 6% sucrose, 0.27 % agar, glutamine and various concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D). After 8 weeks in culture, the best concentration of 2,4-D to induce somatic embryo is at 45.2 ?M. (Author)

  7. Gene Technology for Papaya Ringspot Virus Disease Management

    OpenAIRE

    Md. Abul Kalam Azad; Latifah Amin; Nik Marzuki Sidik

    2014-01-01

    Papaya (Carica papaya) is severely damaged by the papaya ringspot virus (PRSV). This review focuses on the development of PRSV resistant transgenic papaya through gene technology. The genetic diversity of PRSV depends upon geographical distribution and the influence of PRSV disease management on a sequence of PRSV isolates. The concept of pathogen-derived resistance has been employed for the development of transgenic papaya, using a coat protein-mediated, RNA-silencing mechanism and replicase...

  8. Genetic diversity studies of Papaya meleira virus

    Scientific Electronic Library Online (English)

    Cleidiane B., Daltro; Emanuel Felipe Medeiros, Abreu; Francisco Jose Lima, Aragão; Eduardo C., Andrade.

    2014-02-01

    Full Text Available Papaya (Carica papaya) is a fruit crop of great economic and social importance for Brazil and other papaya-producing countries. Brazil is the second largest producer in the world. The papaya sticky disease, caused by Papaya meleira virus (PMeV), has caused great losses in the major Brazilian papaya- [...] producing states. In order to estimate the genetic diversity of PMeV, latex samples were collected from papaya plants in the states of Bahia, Espírito Santo, Pernambuco, Ceará and Rio Grande do Norte, and total RNA was extracted. Specific primer for the replicase region allowed the amplification, by RT-PCR, of a fragment of approximately 560 bp from 31 isolates. The sequence analysis indicated a level of conservation greater than 88% among isolates. Furthermore, comparative analyzes indicated that PMeV has similarity with mycoviruses of the family Totiviridae. This phylogenetic relationship was reinforced by the presence of conserved motifs within in the RdRp regions from mycoviruses.

  9. chilena

    Directory of Open Access Journals (Sweden)

    David E. Hojman

    2005-01-01

    Full Text Available Chile es el único país latinoamericano donde las políticas económicas neoliberales han tenido éxito. Esto podría deberse tanto a la cultura nacional como a la cultura organizacional del país, que están estrechamente relacionadas. Ambas están llenas de contradicciones. El trabajo identifica algunas características más estables en el largo plazo de ambas culturas, así como también dramáticos cambios culturales ocurridos desde mediados de los años ochenta. Éstos últimos coexistieron con rápido crecimiento de la economía. Debido a ambigüedades, inconsistencias y contradicciones en la gestión organizacional, particularmente en lo relativo a recursos humanos, muchos empleados pueden haber sido forzados a adoptar patrones culturales y de comportamiento frecuentemente asociados a la caricatura Condorito

  10. EFFECT OF UNRIPE CARICA PAPAYA ON UTERUS

    Directory of Open Access Journals (Sweden)

    Meera Sumanth

    2013-06-01

    Full Text Available The present study was under taken to establish the uterine stimulant activity of aqueous extract of unripe Carica papaya. The effect of the aqueous extract of unripe Carica papaya was studied on isolated rat uterus and embryo. Uterine stimulant activity was compared with clinically available drug oxytocin and the effect on embryo was compared with misoprostol. In the in vitro model, Carica papaya showed contractile effect on the isolated uterus in diestrus stage and when administered orally in dose of 200 and 400 mg/kg to pregnant rats, resulted in gradual decrease in body weight indicating embryonic resorption. The present study confirms the uterine stimulant activity of aqueous extract of unripe Carica papaya fruit.

  11. Improving Tenderness of Spent Layer Hens Meat Using Papaya Leaves (Carica papaya

    Directory of Open Access Journals (Sweden)

    H. O. Abdalla, N. N. A. Ali, F. S. Siddig and S. A. M. Ali*

    2013-01-01

    Full Text Available Two experiments were performed to study the use of papaya leaves as a meat tenderizer. The first experiment was to evaluate the effect of papaya dry leaves added to hen’s diet before slaughter. Spent hens (n=48 were used, half of them were fed a concentrate ration containing10% dried papaya leaves powder (DPLP while others received layer ration (Control, for 10 days. The second experiment involved a comparison between papaya leaves juice (PLJ, fresh papaya leaves (FPL and vinegar solution (VS as marinades applied to meat for one or two hours before cooking. Spent layer hens (n=42 were used for tenderness evaluation method. After slaughtering and preparing the chickens two methods of cooking were used (oven and moist cooking. The cooked parts (breast, thigh and drumstick were subjected to a panel test evaluation according to a designed questionnaire. Addition of dried papaya leaves powder to spent layer hens ration significantly (P?0.05 increased the level of meat tenderness. Moist cooking had significantly (P?0.05 improved meat tenderization compared to oven cooking. Meat treated with fresh papaya leaves had significantly (P?0.05 improved tenderness. It was concluded that wrapping the tough meat of spent layer hens with fresh papaya leaves for one hour and moist cooking improve tenderness of meat.

  12. Validation and use of DNA markers for sex determination in papaya (Carica papaya)

    International Nuclear Information System (INIS)

    Profitable papaya production requires female and hermaphrodite plants in higher number than male plants. This is only possible if sex of plants is determined at an early growth stage. The present study was conducted to validate sex-linked DNA markers using plants from two Pakistani papaya varieties and subsequently utilize them for determination of sex in juvenile papaya plants. One hundred and five plants (including 49 male and 56 female) of two Pakistani Papaya varieties at flowering stage were screened with six DNA markers viz., W-11, T12, SDP, Napf-76Napf-76, PKBT4 and PKBT5. All male plants exhibited amplification of sex-linked alleles with markers T12 and W11, whereas, 96% and 95% of female plants showed the absence of sex-linked allele with these markers, respectively. Markers SDP, PKBT5 and Napf-76 showed the presence of sex-linked alleles in 98%, 96% and 93% of male plants, respectively, whereas the same markers showed the absence of sex-linked alleles in 100%, 96% and 94% of female plants. One marker, PKBT4 could not produce expected PCR amplification reported previously. The five DNA markers were further used to screen 171 papaya seedlings. These markers clearly differentiated male and female sex types in the studied papaya plants. Results of our study are likely to facilitate Pakistani papaya breeders and growers to incorporate DNA based screening at juvenile stage to determine sex at early stage and to ensure profitable papaya production. (author)

  13. Casa Chilena 1: Rancagua, Chile

    Directory of Open Access Journals (Sweden)

    Smiljan Radic

    2010-08-01

    Full Text Available Los nogales crecidos a los cuales se les ha dejado en paz, la negación del exterior gracias a una tapia perimetral pintada a la cal, un patio interior con posibilidades de ser cubierto por una sombra simple de membrana agrícola anclada a sus muros, un estar familiar austero con síntomas de exterior desplegado a todo lo largo del patio, un cielo de madera en bruto pintada y los dormitorios de colores fuertes, recuerdan de una u otra manera, las casas de inquilinos del campo chileno. En la Casa Chilena 2, se acentúa la negación del exterior y el patio interior se parte en dos generando exteriores matizados.The grown walnut trees left in peace, the negation of the exterior thanks to a whitewashed perimeter wall, an interior patio with the possibility of being covered by the simple shade of an agricultural screen anchored to its walls, an austere, family living room with exterior characteristics unfolding along the patio, a rough, painted wood ceiling and bedrooms in strong colors, remind one of the Inquilino houses of the Chilean countryside. In the Chilean House 2, the negation of the exterior is accentuated and the interior patio divides in two to generate blended exteriors.

  14. Crecimiento, desarrollo y potencial productivo de la papaya (Carica papaya L.) variedad hawaina en el Caribe colombiano / Growth, development and the productive potential of the "Hawaiian" papaya variety (Carica papaya L.)

    OpenAIRE

    Martínez Bustamante, Enrique Guillermo

    1998-01-01

    En este trabajo se definen y cuantifican las diversas etapas de crecimiento y las variaciones entre los estados de desarrollo del árbol de papaya (Carica papaya L.) variedad “Hawaiana” y se conocen las condiciones para obtener crecimiento, desarrollo y rendimiento adecuado y su relación con la producción. / Abstract: Different growth and development variations among phases of the papaya tree (Carica papaya L.) are defined and quantified in this study, besides, both, development and yield are ...

  15. 78 FR 48628 - Importation of Papayas From Peru

    Science.gov (United States)

    2013-08-09

    ...0579-AD68 Importation of Papayas From Peru AGENCY: Animal and Plant Health Inspection...commercial consignments of fresh papayas from Peru into the continental United States. The...conditions for the importation of papayas from Peru would include requirements for...

  16. 78 FR 48628 - Importation of Papayas From Peru

    Science.gov (United States)

    2013-08-09

    ... of Papayas From Peru AGENCY: Animal and Plant Health Inspection Service, USDA. ACTION: Proposed rule... of fresh papayas from Peru into the continental United States. The conditions for the importation of papayas from Peru would include requirements for approved production locations; field sanitation;...

  17. HIGH SPEED CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY (HSCCC) ISOLATION AND IDENTIFICATION BY LC-MSn ANALYSIS OF THE POLAR PHENOLICS FROM VASCONCELLEA QUERCIFOLIA

    Scientific Electronic Library Online (English)

    CRISTINA, QUISPE; EZEQUIEL, VIVEROS-VALDEZ; JOSÉ A, YARLEQUE; MARCO R, ARONES; JUAN C, PANIAGUA; GUILLERMO, SCHMEDA-HIRSCHMANN.

    2013-09-01

    Full Text Available High speed centrifugal countercurrent chromatography (HSCCC) was successfully applied to preparative isolation of the main polar phenolics from the Peruvian Caricaceae Vasconcellea quercifolia A. St.-Hil. High-performance liquid chromatography with diode array (HPLC-DAD) and electrospray ionisation [...] mass spectrometric detection (ESI-MS) was used to carry out a comprehensive characterisation of phenolic compounds from fruits and leaves of this plant. The main phenolics were the quercetin diglycoside rutin and the triglycoside manghaslin. Nine minor flavonoids were tentatively identified as kaempferol, quercetin, isorhamnetin and methoxymyricetin glycosides. HSCCC combined with other chromatographic methods allowed the isolation of the main phenolics from the plant. HPLC-DAD-MS analysis shows that the main phenolic compounds in V. quercifolia are similar to those described for V. pubescens but differs in the identity of the minor constituents.

  18. Effect of gamma irradiation in papaya (Carica papaya L.) harvested in three degrees of maturation

    International Nuclear Information System (INIS)

    The papaya is a fragile tropical fruit with thin skin, susceptible to post harvest diseases and mechanical injuries. Furthermore, it is sensible to low temperatures and at normal conditions it ripens rapidly, increasing the difficulties with storage. The objective of this work was to evaluate the influence of gamma irradiation in papayas harvested in three degrees of maturation, in order to increase shelf life. To accomplish that, papayas were harvested in perfect quality conditions, washed, submitted to carnauba wax and selected by skin coloration into three distinct degrees of maturation: Mat 0, or beginning of yellow coloration; Mat 1, yellow stripes more developed, and Mat 2, one third yellow. Half of them were submitted to irradiation with 0, 75 kGy, while the others became control treatment. They were analyzed in four different periods of conservation, which were 1 day after irradiation (DAI) refrigerated (11 +- 1 deg C), 14 DAI refrigerated, 14 DAI refrigerated + 3 at room temperature (RT = 24 deg +- 2 deg C) and 14 DAI refrigerated + 6 at RT. The effect of irradiation was not influenced by the maturation degree at harvest. Irradiation promoted firmness maintenance of papaya and, therefore, delayed ripening; modified the green color of papaya to a lighter tone, which determined more homogeneity in the skin yellow color development (greater values of L* and b*), and turned the papaya flesh color lighter (rower values of b*). There was no effect of irradiation in papaya weight loss, in the occurrence of diseases, in the development of surface yellow color, in the parameter a* of papaya skin color, in the parameters L*, a* and chroma of flesh color, p H and total soluble solids content. Visual and organoleptic sensorial tests were accomplished with papayas from a new delivery in the conservation period of 14 DAI refrigerated + 3 at RT. In the visual test was evaluated the appearance of papaya in the following treatments: Mat O control, Mat O irradiated, Mat 1 control, Mat 1 irradiated, Mat 2 control e Mat 2 irradiated, in which the first one received lower scores due to the occurrence of ratting on one of the papayas. In the organoleptic test, the parameters firmness, sweetness and acceptance were evaluated for the following treatments: control, Mat O irradiated, Mat 1 irradiated e Mat 2 irradiated. There was no difference between treatments for the sweetness parameter. The irradiated treatments received better scores for firmness as well as papayas irradiated at Mat 1 and 2 for acceptance (author)

  19. Caracterización de accesiones de papaya (Carica papaya L.) a través de marcadores AFLP en Cuba / Characterising Cuban papaya accessions (Carica papaya L.) by AFLP markers

    Scientific Electronic Library Online (English)

    Maruchi Alonso, Esquivel; Martín, Bautista Alor; Matilde, Ortiz García; Adriana, Quiroz Moreno; Wolfgang, Rohde; Lorenzo Felipe, Sánchez Teyer.

    2009-06-01

    Full Text Available Los marcadores moleculares son herramientas valiosas en los estudios genéticos en plantas, y están siendo empleados exitosamente en programas de mejoramiento principalmente en la elección de progenitores y en la selección. El polimorfismo observado mediante la técnica molecular AFLP (Amplified Fragm [...] ent Length Polymorphism) ha sido de utilidad para estudios de diversidad genética en frutales. En el presente trabajo se realizó la caracterización molecular de 12 accesiones de papaya (Carica papaya L.) del banco de germoplasma del Instituto de Investigaciones en Fruticultura Tropical (IIFT), empleando la técnica AFLP. Se evaluaron seis combinaciones de iniciadores para la amplificación selectiva, las cuales amplificaron un total de 431 bandas con 73,3% de polimorfismo. El número total de patrones de bandas identificados fue igual en todas las combinaciones utilizadas, con un porcentaje de identificación alto, lo que sugiere que dichas combinaciones pudieran ser empleadas para estudios de variabilidad genética en papaya. En general, los resultados presentados demuestran que existe diversidad genética entre las accesiones evaluadas, lo cual constituye un reflejo del origen que presentan los genotipos analizados a partir de la introducción de materiales foráneos y la polinización abierta de un grupo de materiales selectos. Por tanto, se recomienda retomar la prospección y selección de accesiones locales, así como la introducción de nuevos genotipos foráneos, como dos vías fundamentales para aumentar la diversidad genética presente en el banco de germoplasma de papaya de Cuba. Abstract in english Molecular markers are valuable tools for genetic studies in plants and they are often used successfully in genetic breeding, mainly for choosing progenitors and selection. Polymorphism observed by amplified fragment length polymorphism (AFLP) has been useful for genetic diversity studies in fruit tr [...] ees. Twelve papaya accessions from the Tropical Fruit Crop Research Institute germplasm bank were molecularly characterised by AFLP. 431 bands having 73.3% polymorphism were obtained using 6 primer combinations. The total number of band patterns identified was the same in all combinations assayed with a high percentage of identification, suggesting that such primer combinations could be used for genetic variability studies in papaya. The results demonstrated genetic diversity among the papaya accessions evaluated, indicating the origin of the analysed genotypes from exogenous material and open pollination of a selected group of material. It is thus recommended that local accessions and their selection be monitored as well as the introduction of new foreign genotypes as two ways of increasing the genetic diversity of the Cuban papaya germplasm bank.

  20. Quantification of the antiplasmodial alkaloid carpaine in papaya (Carica papaya) leaves.

    Science.gov (United States)

    Julianti, Tasqiah; Oufir, Mouhssin; Hamburger, Matthias

    2014-08-01

    Daily consumption of papaya (Carica papaya) leaves as greens and an herbal infusion is common in some parts of Indonesia as a means for preventing malaria. Antiplasmodial activity of the leaf extracts and of the main alkaloid carpaine were recently confirmed. A quantitative assay for determination of carpaine in papaya leaves was developed and validated. The assay involved pressurized liquid extraction and quantification with the aid of ultrahigh-performance liquid chromatography-tandem mass spectroscopy. Extraction conditions were optimized with respect to solvent, temperature, and number of extraction cycles. The ultrahigh-performance liquid chromatography-tandem mass spectroscopy assay was validated over a range of 20-5000 ng/mL (R(2) of 0.9908). A total of 29 papaya leaf samples were analyzed, and carpaine concentration in dry leaves was found to range from 0.02 to 0.31%. No obvious dependence on geographic origin and leaf maturity was observed. PMID:25153096

  1. Uptake of 32P labelled superphosphate by endomycorrhizal papaya (Carica papaya cv. Coorg honey dew)

    International Nuclear Information System (INIS)

    In papaya (Carica papaya cv. Coorg honey dew), there was an increase in 32P uptake and total phosphorus in plants inoculated with mycorrhizal fungi Glomus mossae and G. fasciculatum. Phosphorus derived from fertilizer (Pdff) was lower in mycorrhizal plants while soil derived P, utilisation of P and A values were higher showing thereby that mycorrhizal plants had utilised forms of phosphorus not available to non-mycorrhizal plants. (author). 13 refs., 1 tab

  2. Incidencia y severidad de la antracnosis en líneas e híbridos de papaya (Carica papaya)

    Scientific Electronic Library Online (English)

    Antonio, Bogantes-Arias; Eric, Mora-Newcomer.

    2013-12-01

    Full Text Available Incidencia y severidad de la antracnosis en líneas e híbridos de papaya (Carica papaya). El objetivo de este estudio fue determinar la incidencia y severidad de la antracnosis (Colletotrichum gloeosporioides), en frutas de papaya. El experimento se realizó en Guápiles, Limón, Costa Rica, entre los a [...] ños 2010 y 2011. Se sembraron cinco líneas y cuatro híbridos bajo un diseño de bloques completos al azar con tres repeticiones. Se evaluó la incidencia y severidad de la antracnosis, así como la firmeza y los sólidos solubles (grados Brix) de la fruta. Los híbridos presentaron características intermedias a los padres para las características evaluadas. En la antracnosis de fruta, la resistencia tiende a ser dominante para el material evaluado. Abstract in english Incidence and severity of anthracnose in breeding lines and crosses of papaya (Carica papaya). The objective of this study was to determine the incidence and severity of papaya fruit anthracnose (Colletotrichum gloeosporioides). The experiment was carried out in Guápiles, Limón, Costa Rica, between [...] the years 2010 and 2011. Five lines and four crosses were evaluated in a randomized complete block design with three replications. Evaluated traits were incidence and severity of anthracnose, fruit firmness and total soluble solids content of the pulp. Results suggest that the crosses have intermediate characteristics to those of their parent lines for these traits. In the specific case of anthracnose, evidence also suggests that the resistance to this disease tends to be inherited in a dominant manner.

  3. Literatura chilena de exilio, un vacío epistemológico

    Directory of Open Access Journals (Sweden)

    Sol Marina Garay C

    2013-06-01

    Full Text Available Este artículo se presenta como una reflexión acerca del desinterés actual que supone, dentro del panorama literario en Chile, el estudio de la literatura creada por autores y autoras chilenas durante el exilio chileno ocurrido entre 1973 y 1989. Una reflexión que espero sirva como referente para aquellos investigadores que se interesen por estudiar y dar a conocer los diversos corpus literarios vinculados al exilio chileno vivido en diferentes países del mundo. Una producción literaria aún por desvelar y que agrupa un conjunto de corpus literarios que nos hablan del pasado reciente de Chile y sus habitantes, una literatura chilena de exilio en la cual las experiencias de dolor y desarraigo dan paso a la apertura al otro y al encuentro con diversas memorias culturales.

  4. Nematodos fitoparásitos asociados al cultivo de papaya (Carica papaya L.) en Colima, México / Phytoparasitic nematodes associated with the cultivation of papaya (Carica papaya L.) in Colima, Mexico

    Scientific Electronic Library Online (English)

    José Ángel, Martínez Gallardo; Tomás, Díaz Valdés; Raúl, Allende Molar; José Alfonso, Ortiz Meza; Raymundo Saúl, García Estrada; José Armando, Carrillo Fasio.

    2014-03-01

    Full Text Available Este estudio tuvo como objetivo detectar e identificar los géneros de nematodos fitoparásitos asociados al cultivo de papaya (Caricapapaya L.) en los municipios de Tecomán y Colima, Colima. Se seleccionaron diez huertos, de siete ranchos en los que se colectaron muestras de raíces y suelo rizosféric [...] o; en cada muestra se cuantificó e identificaron las poblaciones de los fitonematodos. Para la identificación específica de Meloidogyne, se analizaron cortes perineales de las hembras y se confirmó por reacción en cadena de la polimerasa (PCR). Los géneros de fitonematodos detectados e identificados fueron: Aphelenchus, Ditylenchus, Helicotylenchus, Paratylenchus, Pratylenchus, Rotylenchulus, Rotylenchus, Trophurus, Tylenchorrhynchus, Tylenchus, y Meloidogyne, con la especie M. incognita. Los resultados permiten el reporte por primera vez de M. incognita, asociado al cultivo de papaya en Colima, México. Abstract in english This study aimed to detect and identify phytoparasitic nematodes associated with the cultivation of papaya (Caricapapaya L.) in the municipalities of Tecomán and Colima, Colima. We selected ten orchards, seven ranches in which samples were collected roots and rhizosphere soil, in each sample was qua [...] ntified and identified populations of phytoparasitic nematodes. For specific identification of Meloidogyne, perineal cuts were analyzed and confirmed females by chain reaction (PCR). The genera of phytoparasitic nematodes were detected and identified: Aphelenchus, Ditylenchus, Helicotylenchus, Paratylenchus, Pratylenchus, Rotylenchulus, Rotylenchus, Trophurus, Tylenchorrhynchus, Tylenchus, and Meloidogyne, with the species M. incognita. The results allow the first report of M incognita associated with papaya in Colima, Mexico.

  5. Transgenic Papaya: Development, Release, Impact, and Challenges

    Science.gov (United States)

    Although the technology for developing virus-resistant transgenic plants through the use of the coat protein of a virus was unveiled twenty years ago, it is surprising to note that only a three virus-resistant plants (squash, potato, and papaya) have been commercialized in the U.S. The transgenic p...

  6. Papaya seed represents a rich source of biologically active isothiocyanate.

    Science.gov (United States)

    Nakamura, Yoshimasa; Yoshimoto, Motoko; Murata, Yoshiyuki; Shimoishi, Yasuaki; Asai, Yumi; Park, Eun Young; Sato, Kenji; Nakamura, Yasushi

    2007-05-30

    In the present study, papaya (Carica papaya) seed and edible pulp were carefully separated and then the contents of benzyl isothiocyanate and the corresponding glucosinolate (benzyl glucosinolate, glucotropaeolin) quantified in each part. The papaya seed with myrosinase inactivation contained >1 mmol of benzyl glucosinolate in 100 g of fresh seed. This content is equivalent to that of Karami daikon (the hottest Japanese white radish) or that of cress. The papaya seed extract also showed a very high activity of myrosinase and, without myrosinase inactivation, produced 460 micromol of benzyl isothiocyanate in 100 g of seed. In contrast, papaya pulp contained an undetectable amount of benzyl glucosinolate and showed no significant myrosinase activity. The n-hexane extract of the papaya seed homogenate was highly effective in inhibiting superoxide generation and apoptosis induction in HL-60 cells, the activities of which are comparable to those of authentic benzyl isothiocyanate. PMID:17469845

  7. CARICA PAPAYA: A COMPLETE PACKAGE OF NUTRITIONALAND MEDICINAL BENEFITS

    Directory of Open Access Journals (Sweden)

    Karabhari Rekha Bhaskar

    2014-07-01

    Full Text Available Papaya (Carica papaya Linn. is well known for its exceptional nutritional and medicinal properties throughout the world. From the times immemorial, the whole Carica papaya plant including its leaves, seeds, ripe and unripe fruits and their juice is used as traditional medicine. The fruit has a large oval shape, yellowish -green skin and yellow flesh. Now a day, Carica papaya is considered as nutraceutical fruit due to its multi faceted medicinal and nutritional properties. The prominent medicinal properties of Carica papaya include anti-fertility, uterotonic, and diuretic, anti-hypertensive, wound healing, anti bacterial activities. Nutritionally the whole plant contains enzymes, vitamin A, vitamin C, B-complex vitamins and potassium. The present article reviews the nutritional and medicinal uses of Carica papaya.

  8. The draft genome of the transgenic tropical fruit tree papaya (Carica papaya Linnaeus)

    Science.gov (United States)

    Ming, Ray; Hou, Shaobin; Feng, Yun; Yu, Qingyi; Dionne-Laporte, Alexandre; Saw, Jimmy H.; Senin, Pavel; Wang, Wei; Ly, Benjamin V.; Lewis, Kanako L. T.; Salzberg, Steven L.; Feng, Lu; Jones, Meghan R.; Skelton, Rachel L.; Murray, Jan E.; Chen, Cuixia; Qian, Wubin; Shen, Junguo; Du, Peng; Eustice, Moriah; Tong, Eric; Tang, Haibao; Lyons, Eric; Paull, Robert E.; Michael, Todd P.; Wall, Kerr; Rice, Danny W.; Albert, Henrik; Wang, Ming-Li; Zhu, Yun J.; Schatz, Michael; Nagarajan, Niranjan; Acob, Ricelle A.; Guan, Peizhu; Blas, Andrea; Wai, Ching Man; Ackerman, Christine M.; Ren, Yan; Liu, Chao; Wang, Jianmei; Wang, Jianping; Na, Jong-Kuk; Shakirov, Eugene V.; Haas, Brian; Thimmapuram, Jyothi; Nelson, David; Wang, Xiyin; Bowers, John E.; Gschwend, Andrea R.; Delcher, Arthur L.; Singh, Ratnesh; Suzuki, Jon Y.; Tripathi, Savarni; Neupane, Kabi; Wei, Hairong; Irikura, Beth; Paidi, Maya; Jiang, Ning; Zhang, Wenli; Presting, Gernot; Windsor, Aaron; Navajas-Pérez, Rafael; Torres, Manuel J.; Feltus, F. Alex; Porter, Brad; Li, Yingjun; Burroughs, A. Max; Luo, Ming-Cheng; Liu, Lei; Christopher, David A.; Mount, Stephen M.; Moore, Paul H.; Sugimura, Tak; Jiang, Jiming; Schuler, Mary A.; Friedman, Vikki; Mitchell-Olds, Thomas; Shippen, Dorothy E.; dePamphilis, Claude W.; Palmer, Jeffrey D.; Freeling, Michael; Paterson, Andrew H.; Gonsalves, Dennis; Wang, Lei; Alam, Maqsudul

    2010-01-01

    Papaya, a fruit crop cultivated in tropical and subtropical regions, is known for its nutritional benefits and medicinal applications. Here we report a 3× draft genome sequence of ‘SunUp’ papaya, the first commercial virus-resistant transgenic fruit tree1 to be sequenced. The papaya genome is three times the size of the Arabidopsis genome, but contains fewer genes, including significantly fewer disease-resistance gene analogues. Comparison of the five sequenced genomes suggests a minimal angiosperm gene set of 13,311. A lack of recent genome duplication, atypical of other angiosperm genomes sequenced so far2–5, may account for the smaller papaya gene number in most functional groups. Nonetheless, striking amplifications in gene number within particular functional groups suggest roles in the evolution of tree-like habit, deposition and remobilization of starch reserves, attraction of seed dispersal agents, and adaptation to tropical daylengths. Transgenesis at three locations is closely associated with chloroplast insertions into the nuclear genome, and with topoisomerase I recognition sites. Papaya offers numerous advantages as a system for fruit-tree functional genomics, and this draft genome sequence provides the foundation for revealing the basis of Carica's distinguishing morpho-physiological, medicinal and nutritional properties. PMID:18432245

  9. COMPARATIVE STUDIES ON ANTHELMINTIC POTENTIAL OF CUCURBITA MAXIMA (PUMPKIN SEEDS AND CARICA PAPAYA (PAPAYA SEEDS

    Directory of Open Access Journals (Sweden)

    Sengupta Rupa

    2013-08-01

    Full Text Available The crude extract of Carica papaya (papaya seeds (CP and Cucurbita maxima (Pumpkin seeds (CM were assayed against adult earthworms (Pheretima posthuma for the evaluation of anthelmintic activity. Various concentrations of both extracts were tested and results were expressed in terms of time for paralysis (P and time for death (D of worms. Albendazole was used as a reference standard. The result showed that in both of the extracts (i.e. CP and CM dose of 60 mg / ml possesses more wormicidal activity. The time of paralysis was 1.88 ± 0.52 minute and 1.93 ± 0.57 minute whereas the time of death was 3.45 ± 0.17 minute and 4.90 ± 0.18 minute in the case of Carica papaya and Cucurbita maxima respectively. In conclusion, the use of seeds of Carica papaya (CP and Cucurbita maxima (CM for anthelmintic activity have been confirmed and further studies are suggested to isolate the active principles responsible for the activity. Both the extracts showed significant anthelmintic activity, but the comparative study showed that out of these two, Carica papaya proves to be a better anthelmintic remedy.

  10. Influence of ripening stages on antioxidant properties of papaya fruit (Carica papaya L.)

    Science.gov (United States)

    Addai, Zuhair Radhi; Abdullah, Aminah; Mutalib, Sahilah Abd.

    2013-11-01

    Papaya (Carica papaya L. cv Eksotika) is one of the most commonly consumed tropical fruits by humans, especially Malaysians. The objective of this study was to determine the phenolic compounds and antioxidants activity in different ripening stages of papaya fruit. The fruits were harvested at five different, stages RS1, RS2, RS3, RS4, and RS5 corresponding to 12, 14, 16, 18, and 20 weeks after anthesis, respectively. Papayas fruit at five different stage of ripening were obtained from farms at Pusat Flora Cheras, JabatanPertanian and Hulu Langat Semenyih, Selangor, Malaysia. The antioxidants activity were analyzed using the total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant Power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). The analyses were conducted in triplicate and the data were subjected to statistical analysis using SPSS. The results showed significant differences (P< 0.05) were found at different stages of ripening. The total phenol content TPC, TFC, FRAP and DPPH values increased significantly (P<0.05) with the ripening process. The results showed the important role of the ripening stage in increasing the antioxidant content of papaya fruits.

  11. Fusarium species associated with fruit rot of banana (Musa spp.), papaya (Carica papaya) and guava (Psidium guajava)

    OpenAIRE

    Zakaria, L.; Mazzura, W. C.; Kong, W. H.; Baharuddin, S.

    2012-01-01

    A total of 60 isolates of Fusarium were isolated from fruit rot of banana (Musa spp.), papaya (Carica papaya) and guava(Psidium guajava). The most common species recovered from the fruit rot of the three fruit crops were F. semitectum(40 %), F. solani (38.3 %), F. verticillioides (11.7 %) and F. oxysporum (10 %). Fusarium semitectum was isolated from fruit rot of banana, papaya and guava; F. oxysporum from banana and papaya; F. solani from banana and guava and F.verticillioides from banana. F...

  12. Extraction and characteristics of seed oil from Papaya (Carica papaya in Congo-Brazzaville

    Directory of Open Access Journals (Sweden)

    G. Bouanga-Kalou

    2011-03-01

    Full Text Available Papaya seeds were collected and dried. This study was carried out on papaya seed to clarify their proximate composition and the characteristics of the extracted oil including unsaponifiable matter and fatty acid composition. The seed is a rich source of protein (26.78% and crude fiber (21.4%. Palmitic acid was the main saturated fatty acid (15.22%, while linoleic acid was the major unsaturated fatty acid (76.38% in all lipid classes. The physical properties of the oil extracts showed the state to be liquid at room temperature. Carica papaya seeds have ash content of 3.2% (with the presence of following minerals: K, Na, Ca, P and Mg. However, Ca and P occur in appreciable quantities (1821±2.12 mg/100 g dry matter and 1156±1.8 mg/100 g dry mater, respectively.

  13. Evaluación de la calidad y el rendimiento en papaya silvestre (Carica papaya L.) de Cuba / Evaluation of quality and yield in papaya wild (Carica papaya L.) from Cuba

    Scientific Electronic Library Online (English)

    Jesús, Rodríguez Cabello; Yusnier, Díaz Hernández; Aymara, Pérez González; Zulma, Natali Cruz; Pedro, Rodríguez Hernández.

    2014-09-01

    Full Text Available La papaya (Carica papaya L.) de la familia Caricaceae, es nativa del trópico americano y la especie más importante del género Carica por su alto valor nutritivo e industrial. En sus áreas de origen existen parientes silvestres que proporcionan alimento a las comunidades que las mantienen y de ser ap [...] rovechadas, podrían constituir una importante fuente de ingresos debido a que poseen genes útiles para elevar el rendimiento y calidad nutricional de sus frutos. Existe referencia de las poblaciones de papaya silvestres en nuestro país. Sin embargo, aún no se han caracterizado o evaluado con profundidad, de modo que se desconoce el rendimiento y la calidad de sus frutos. El presente estudio se llevó a cabo con el propósito de evaluar genotipos de papaya silvestre, prospectados en las alturas del nacimiento de las Cuencas Almendares-Vento y Noreste de la Habana, a través de indicadores físicos, químicos y el rendimiento, que permitan valorar su aceptación en el mercado. Los resultados mostraron alto rendimiento y frutos con características que les permiten ser de gran aceptación en el mercado para consumo en fresco por su masa, forma, dimensiones, sólidos solubles totales y color de la cáscara y la pulpa. Además, las características apreciadas favorecen su empleo para futuros programas de mejoramiento genético en Cuba, con el propósito de obtener cultivares con alto número de frutos de mediano a pequeño tamaño por planta Abstract in english The papaya (Carica papaya L.), family Caricaceae, is native from American tropic and the most important in the gender Carica for its nutritious and industrial high value. In their origin areas wild relatives exist, that provide food to the communities that maintain them, could constitute an importan [...] t source of revenues, because they possess useful genes to elevate the yield and nutritional quality of their fruits. The wild papaya populations’ reference exists in our country. However, they have not still been characterized or evaluated with depth, so one ignores the yield and the quality of their fruits. The present study was carried out with the purpose of evaluating genotypes of wild papaya, prospected in the heights of the birth of the Basins Almendares-Vento and Northeast of Havana, through physical, chemical indicators and the yield, that allow to value their acceptance in the market. The results showed high yield and fruits with characteristics that allow them to be of great acceptance in the market for consumption in fresh for their mass, it forms, dimensions, soluble total solids and color of the shell and the pulp. Also, the appreciated characteristics favor their employment for futures programs of genetic improvement in Cuba, with the purpose of obtaining cultivares with high number of fruits of medium to small size for plant

  14. Whole-photosynthesis and transpiration in field-grown papaya plants

    Science.gov (United States)

    Papaya (Carica papaya L.) is a principal horticultural crop of tropical and subtropical regions. Knowledge of papaya response to environmental factors provides a scientific basis to develop management strategies to optimize fruit yield and quality. In papaya, the photosynthetic capacity also influ...

  15. Virus Resistant Transgenic Papaya: Commercial Development and Regulatory and Environmental Issues

    Science.gov (United States)

    In Hawaii, transgenic papaya resistant to Papaya ringspot virus (PRSV) was developed starting in the 1980s and released commercially in 1998 to combat the wide spread destruction of Hawaii’s papaya industry. This review describes the proactive development of the transgenic papaya and its impact on ...

  16. Dwarf mutant of papaya (Carica papaya L. ) induced by gamma rays

    Energy Technology Data Exchange (ETDEWEB)

    Ram, M. (Indian Agricultural Research Inst., Pusa. Regional Research Station)

    1981-09-01

    Treating the seeds of papaya with 15 Krad of gamma rays, three plants were isolated from the M/sub 3/ population. Repeated full sibmating among the progenies of these dwarf mutants helped in establishing a homozygous population of dwarf papaya in M/sub 6/ generation. Though the per plant yield in mutant is less, nevertheless its importance is envisaged in the fact that it is likely to incur less damage by wind and storm, can be better managed and more number of plants can be accomodated per unit area, thus giving increased yield per hectare. 5 refs.

  17. Extraction and characteristics of seed oil from Papaya (Carica papaya) in Congo-Brazzaville

    OpenAIRE

    G. Bouanga-Kalou; A. Kimbonguila; J.M. Nzikou; F.B. Ganongo-Po; F.E. Moutoula; E. Panyoo-Akdowa; Th. Silou and S. Desobry

    2011-01-01

    Papaya seeds were collected and dried. This study was carried out on papaya seed to clarify their proximate composition and the characteristics of the extracted oil including unsaponifiable matter and fatty acid composition. The seed is a rich source of protein (26.78%) and crude fiber (21.4%). Palmitic acid was the main saturated fatty acid (15.22%), while linoleic acid was the major unsaturated fatty acid (76.38%) in all lipid classes. The physical properties of the oil extracts showed the ...

  18. Gamma radiation in papaya harvested at three stages of maturation

    International Nuclear Information System (INIS)

    Papaya is a fragile, perishable fruit, highly accepted worldwide. To keep the quality of papaya from harvest to the consumers, conservation techniques are often used; among them is the application of gamma irradiation. The objective of this work was to evaluate gamma irradiation in papayas harvested at three degrees of maturation, in order to increase shelf life. Papayas were harvested in perfect quality conditions and selected by skin coloration into three distinct degrees of maturation: maturation 0, or beginning of yellow coloration; maturation 1, yellow stripes more developed, and maturation 2, one third yellow. Half of them were irradiated with 0.75 kGy, while the other half became control treatment. They were analyzed in four periods of conservation, which were 1 DAI (days after irradiation refrigerated at 11 ± 1 deg C), 14 DAI, 14 DAI + 3 DRT (room temperature at 24 deg C ± 2 deg C) and 14 DAI + 6 DRT. The papaya maturation degree at harvest did not influence the radiation effect. Irradiation maintained firmness of papaya and, therefore, delayed ripening; modified the green color of papaya to a lighter, more intense tone, which determined more homogeneity in the development of the skin's yellow color (greater values of L* and chroma). There was no effect of irradiation in papaya weight loss, occurrence of diseases, chroma of flesh color, p H and total soluble solids contents. (author)

  19. EVALUACIÓN DE LA CALIDAD Y EL RENDIMIENTO EN PAPAYA SILVESTRE (Carica papaya L. DE CUBA

    Directory of Open Access Journals (Sweden)

    Jesús Rodríguez Cabello

    2014-01-01

    Full Text Available La papaya (Carica papaya L. de la familia Caricaceae, es nativa del trópico americano y la especie más importante del género Carica por su alto valor nutritivo e industrial. En sus áreas de origen existen parientes silvestres que proporcionan alimento a las comunidades que las mantienen y de ser aprovechadas, podrían constituir una importante fuente de ingresos debido a que poseen genes útiles para elevar el rendimiento y la calidad nutricional de sus frutos. Existe referencia de las poblaciones de papaya silvestres en nuestro país. Sin embargo, aún no se han caracterizado o evaluado con profundidad, de modo que se desconoce el rendimiento y la calidad de sus frutos. El presente estudio se llevó a cabo con el propósito de evaluar genotipos de papaya silvestre, prospectados en las alturas del nacimiento de las Cuencas Almendares-Vento y Noreste de la Habana, a través de indicadores físicos, químicos y el rendimiento, que permitan valorar su aceptación en el mercado. Los resultados mostraron alto rendimiento y frutos con características que les permiten ser de gran aceptación en el mercado para consumo en fresco por su masa, forma, dimensiones, sólidos solubles totales y color de la cáscara y la pulpa. Además, las características apreciadas favorecen su empleo para futuros programas de mejoramiento genético en Cuba, con el propósito de obtener cultivares con alto número de frutos de mediano a pequeño tamaño por planta.|

  20. Compostos fenólicos inibidores da germinação em sementes de mamão (Carica papaya Phenolic compound inhibitors in papaya seeds (Carica papaya L.

    Directory of Open Access Journals (Sweden)

    Daí Tokuhisa

    2007-01-01

    Full Text Available Sementes de mamão germinam lenta e irregularmente, o que tem sido atribuído à presença da sarcotesta. O trabalho teve como objetivos avaliar o efeito inibitório de extratos de sarcotesta na germinação de sementes de alface e quantificar compostos fenólicos em sementes de mamão. Foram utilizados frutos de mamão do grupo Formosa, híbrido Tainung 01, colhidos em Maio e Setembro/2005, obtendo-se sementes com e sem sarcotesta que foram avaliadas quanto à germinação aos 15 e 30 dias após a semeadura. Realizou-se um bioensaio com sementes de alface, que foram colocadas para germinar em substrato umedecido com água e com soluções obtidas a partir de extrato da sarcotesta de sementes de mamão. Determinou-se, também, o conteúdo de compostos fenólicos nas diferentes estruturas destas (sarcotesta, esclerotesta, endosperma e embrião, utilizando-se o ácido tânico como padrão e para reação de coloração, o reagente Folin-Ciacalteau, com leitura em espectrofotômetro a 765 nm. Verificou-se que o extrato de sarcotesta inibe a germinação e o crescimento da raiz primária das plântulas de alface, devido à presença de compostos fenólicos. Maior quantidade destes compostos foi observada na esclerotesta das sementes de mamão, seguida da sarcotesta, sendo praticamente nula a presença de tais compostos no embrião e no endosperma. Sementes oriundas da colheita de Maio/2005 exibiram dormência, apresentando maior conteúdo de fenóis na sarcotesta quando comparadas às sementes obtidas de frutos colhidos em Setembro/2005, as quais não apresentaram dormência.The slow and erratic germination of papaya seeds has been attributed to inhibitors present in the sarcotesta. This study was undertaken to evaluate the inhibition effect of sarcotesta extract on lettuce seed germination and to quantify phenolic compounds in papaya seeds. Papaya fruits of the Formosa group, hybrid Tainung 01, were harvested in May and September/2005. At each harvest time, seeds with and without sarcotesta were obtained and the germination percentage was evaluated at 15 and 30 days after seeding. A bioassay was performed with lettuce seeds, which germinated in substratum moistened with water (control and with solutions obtained from papaya sarcotesta extract. The phenolic compounds were quantified in different structures of papaya seeds (sarcotesta, esclerotesta, endosperm and embryo, using tannic acid as standard and the color reaction with Folin-Ciacalteau reagent measured at 765 nm by spectrophotometry. The bioassay indicated that sarcotesta extract inhibits the germination and growth of the lettuce seedling primary roots due to the presence of inhibitory substances (phenols. A high amount of these substances was found in the papaya seed esclerotesta, followed by the sarcotesta, but there was practically no concentration of these compounds in the embryo and endosperm. Seeds extracted from fruits harvested in May 2005 had dormancy and higher phenolic content compared to seeds harvested in September 2005, which did not exhibit dormancy.

  1. Compostos fenólicos inibidores da germinação em sementes de mamão (Carica papaya) / Phenolic compound inhibitors in papaya seeds (Carica papaya L.)

    Scientific Electronic Library Online (English)

    Daí, Tokuhisa; Denise Cunha Fernandes dos Santos, Dias; Eveline Mantovani, Alvarenga; Paulo Cèsar, Hilst; Antônio Jacinto, Demuner.

    Full Text Available Sementes de mamão germinam lenta e irregularmente, o que tem sido atribuído à presença da sarcotesta. O trabalho teve como objetivos avaliar o efeito inibitório de extratos de sarcotesta na germinação de sementes de alface e quantificar compostos fenólicos em sementes de mamão. Foram utilizados frut [...] os de mamão do grupo Formosa, híbrido Tainung 01, colhidos em Maio e Setembro/2005, obtendo-se sementes com e sem sarcotesta que foram avaliadas quanto à germinação aos 15 e 30 dias após a semeadura. Realizou-se um bioensaio com sementes de alface, que foram colocadas para germinar em substrato umedecido com água e com soluções obtidas a partir de extrato da sarcotesta de sementes de mamão. Determinou-se, também, o conteúdo de compostos fenólicos nas diferentes estruturas destas (sarcotesta, esclerotesta, endosperma e embrião), utilizando-se o ácido tânico como padrão e para reação de coloração, o reagente Folin-Ciacalteau, com leitura em espectrofotômetro a 765 nm. Verificou-se que o extrato de sarcotesta inibe a germinação e o crescimento da raiz primária das plântulas de alface, devido à presença de compostos fenólicos. Maior quantidade destes compostos foi observada na esclerotesta das sementes de mamão, seguida da sarcotesta, sendo praticamente nula a presença de tais compostos no embrião e no endosperma. Sementes oriundas da colheita de Maio/2005 exibiram dormência, apresentando maior conteúdo de fenóis na sarcotesta quando comparadas às sementes obtidas de frutos colhidos em Setembro/2005, as quais não apresentaram dormência. Abstract in english The slow and erratic germination of papaya seeds has been attributed to inhibitors present in the sarcotesta. This study was undertaken to evaluate the inhibition effect of sarcotesta extract on lettuce seed germination and to quantify phenolic compounds in papaya seeds. Papaya fruits of the Formosa [...] group, hybrid Tainung 01, were harvested in May and September/2005. At each harvest time, seeds with and without sarcotesta were obtained and the germination percentage was evaluated at 15 and 30 days after seeding. A bioassay was performed with lettuce seeds, which germinated in substratum moistened with water (control) and with solutions obtained from papaya sarcotesta extract. The phenolic compounds were quantified in different structures of papaya seeds (sarcotesta, esclerotesta, endosperm and embryo), using tannic acid as standard and the color reaction with Folin-Ciacalteau reagent measured at 765 nm by spectrophotometry. The bioassay indicated that sarcotesta extract inhibits the germination and growth of the lettuce seedling primary roots due to the presence of inhibitory substances (phenols). A high amount of these substances was found in the papaya seed esclerotesta, followed by the sarcotesta, but there was practically no concentration of these compounds in the embryo and endosperm. Seeds extracted from fruits harvested in May 2005 had dormancy and higher phenolic content compared to seeds harvested in September 2005, which did not exhibit dormancy.

  2. Effect of Biopreservatives on Storage Life of Papaya (Carica papaya L.

    Directory of Open Access Journals (Sweden)

    Fatema H. Brishti

    2013-04-01

    Full Text Available Normal 0 false false false EN-US X-NONE X-NONE In this experiment the effect on post-harvest preservation of papaya (Carica papaya L. fruit coated with either Aloe gel (AG; 100% or papaya leaf extract with Aloe gel (PLEAG; 1:1 was studied. To evaluate the role of coating on ripening behavior and quality of papaya the uncoated and coated fruits were stored and ripened at room temperature (25 °C-29 °C and 82-84% relative humidity. Physico-chemical properties were analyzed at 4 day intervals during the storage period. The incidence of disease attack was also visually observed. The overall results showed the superiority of AG and PLEAG coating in lengthening the shelf-life of papaya fruit compared to controls which showed significant decay from 6th day onward and complete decay within 12 days of storage. The AG and PLEAG coated fruits maintained their shelf life for 12 days and decayed at 16th day. The coated fruits also maintained their color, flavor and firmness up to 12 days of storage. An increase in ascorbic acid content (120.2 mg/100 g was also found in coated fruits in contrast to the control (59 mg/100 g. Only 27% disease incidence was observed in AG and 13% in PLEAG coated fruits as compared to control (100% during the storage period. The results of this study show that both AG and PLEAG coatings have excellent potential to be used on fresh produce to maintain quality and extend shelf-life.

  3. Ranking de universidades chilenas: un análisis multivariado

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    Firinguetti Limone, Luis

    2015-06-01

    Full Text Available In this work a ranking of Chilean universities on the basis of publicly available information is developed. This ranking takes into account the multivariate character of these institutions. Also, it is noted that the results are consistent with those of a well-known international ranking that uses a different set of data, as well as with several multivariate analyses of the data considered in this study.En este trabajo se elabora un ranking de las universidades chilenas en base a información pública disponible. Dicho ranking toma en cuenta el carácter multivariado de estas instituciones. Además, se ha comprobado que los resultados del ranking son consistentes con un conocido ranking internacional construido a partir de un conjunto diferente de datos y con varios análisis multivariados realizados de la información tratada en este estudio.

  4. Tendencias en la historiografía eclesiástica Chilena

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    Camus Ibacache, Misael

    2001-12-01

    Full Text Available The article studies the lines of Chilean ecclesiastical history in XIX and XX. During both centuries the studies are centered in: Independence, Church-State relations, missions, editions of sinodal and pastoral texts. The A. values the influence of the liberal historiography in ecclesiastical history and emphasizes the existence of a new current that denominates pastoral historiography and describes the new sources that are used.

    Estudia las corrientes de la historia eclesiástica chilena en los siglos XIX y XX. Durante ambos siglos los estudios se centran en la Independencia, relaciones Iglesia-Estado, misiones, ediciones de textos conciliares y ediciones de textos pastorales. El A. valora la influencia de la historiografía liberal en la historia eclesiástica y destaca la existencia de una nueva corriente que denomina historiografía pastoral y describe sus fuentes.

  5. Origin and domestication of papaya Yh chromosome

    OpenAIRE

    VanBuren, Robert; Zeng, Fanchang; Chen, Cuixia; Zhang, Jisen; Wai, Ching Man; Han, Jennifer; Aryal, Rishi; Gschwend, Andrea R; Wang, Jianping; Na, Jong-Kuk; Huang, Lixian; Zhang, Lingmao; Miao, Wenjing; Gou, Jiqing; Arro, Jie

    2015-01-01

    Sex in papaya is controlled by a pair of nascent sex chromosomes. Females are XX, and two slightly different Y chromosomes distinguish males (XY) and hermaphrodites (XYh). The hermaphrodite-specific region of the Yh chromosome (HSY) and its X chromosome counterpart were sequenced and analyzed previously. We now report the sequence of the entire male-specific region of the Y (MSY). We used a BAC-by-BAC approach to sequence the MSY and resequence the Y regions of 24 wild males and the Yh region...

  6. Radiation-induced mutation breeding of papaya

    International Nuclear Information System (INIS)

    Irradiation-induced mutation breeding of papaya commenced at the Malaysian Agricultural Research and Development Institute (MARDI) in August, 2000. This research was initiated under a Coordinated Research Project (CRP - D23023) with assistance from the International Atomic Energy Agency (IAEA). In the preliminary dosimetry study, seeds from two local papaya varieties, Sekaki and Eksotika were irradiated, either as dry seeds or as pre-soaked seeds (soaked overnight in water and surface-dried) with radiation doses ranging from 0 to 300 Gy. 100 Gy dose was lethal for all wet presoaked seeds while dry seed did not show loss of viability, even at 300 Gy. From the growth data it was estimated that dose of 525 Gy reduced shoot elongation by 50%, and this dose was recommended for mass irradiation of dry seeds. For wet, pre-soaked seeds results indicated that 42.5 Gy was the optimal dose for mass irradiation. At this dose, both seeds germination and seedlings growth were reduced by 50%. In a massive irradiation experiment 2,000 Eksotika seeds were irradiated at 42.5 Gy (pre-soaked) and another 2,000 at 525 Gy (dry). In the M2 population, numerous physiological defects were observed, including stem splitting, leaf variegation and puckering, and crinkled dwarfs. In the M3 population, a wide variability was recorded for a number of traits. M3 seedlings derived from presoaked seeds irradiated a low 42.5 Gy dose presented a high number of plants that were shorter and more vigorous in leaf development compared to those irradiated at 525 Gy and to non-irradiated control seedlings. The distribution patterns of M3 progenies for nine quantitative field characters showed great variation, often exceeding the limits of the control population. There appears to be good prospects in improving Eksotika papaya especially in the development of dwarf trees with lower fruit bearing stature, higher total soluble solids in fruits and larger fruit size. Several M2 and M3 putative mutants also showed very good resistance to malformed top disease. However, no resistance to papaya ringspot virus disease was found in the 1920 M2 seedlings that were inoculated. (author)

  7. Whole genome sequence analysis of unidentified genetically modified papaya for development of a specific detection method.

    Science.gov (United States)

    Nakamura, Kosuke; Kondo, Kazunari; Akiyama, Hiroshi; Ishigaki, Takumi; Noguchi, Akio; Katsumata, Hiroshi; Takasaki, Kazuto; Futo, Satoshi; Sakata, Kozue; Fukuda, Nozomi; Mano, Junichi; Kitta, Kazumi; Tanaka, Hidenori; Akashi, Ryo; Nishimaki-Mogami, Tomoko

    2016-08-15

    Identification of transgenic sequences in an unknown genetically modified (GM) papaya (Carica papaya L.) by whole genome sequence analysis was demonstrated. Whole genome sequence data were generated for a GM-positive fresh papaya fruit commodity detected in monitoring using real-time polymerase chain reaction (PCR). The sequences obtained were mapped against an open database for papaya genome sequence. Transgenic construct- and event-specific sequences were identified as a GM papaya developed to resist infection from a Papaya ringspot virus. Based on the transgenic sequences, a specific real-time PCR detection method for GM papaya applicable to various food commodities was developed. Whole genome sequence analysis enabled identifying unknown transgenic construct- and event-specific sequences in GM papaya and development of a reliable method for detecting them in papaya food commodities. PMID:27006240

  8. Antioxidant Activity of Papaya Seed Extracts

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    Haofu Dai

    2011-07-01

    Full Text Available The antioxidant activities of the ethanol, petroleum ether, ethyl acetate, n-butanol and water extract fractions from the seeds of papaya were evaluated in this study. The ethyl acetate fraction showed the strongest DPPH and hydroxyl free radical-scavenging activities, and its activities were stronger than those of ascorbic acid and sodium benzoate, respectively. The n-butanol fraction demonstrated the greatest ABTS+ radicals scavenging activity. The ethyl acetate fraction and the n-butanol fraction not only showed higher antioxidant activities than the petroleum ether fraction, water fraction and ethanol fraction, but also showed higher superoxide anion and hydrogen peroxide radicals scavenging activities than those of the other extract fractions. The high amount of total phenolics and total flavonoids in the ethyl acetate and n-butanol fractions contributed to their antioxidant activities. The ethyl acetate fraction was subjected to column chromatography, to yield two phenolic compounds, p-hydroxybenzoic acid (1 and vanillic acid (2, which possessed significant antioxidant activities. Therefore, the seeds of papaya and these compounds might be used as natural antioxidants.

  9. Antioxidant activity of papaya seed extracts.

    Science.gov (United States)

    Zhou, Kaibing; Wang, Hui; Mei, Wenli; Li, Xiaona; Luo, Ying; Dai, Haofu

    2011-01-01

    The antioxidant activities of the ethanol, petroleum ether, ethyl acetate, n-butanol and water extract fractions from the seeds of papaya were evaluated in this study. The ethyl acetate fraction showed the strongest DPPH and hydroxyl free radical-scavenging activities, and its activities were stronger than those of ascorbic acid and sodium benzoate, respectively. The n-butanol fraction demonstrated the greatest ABTS? radicals scavenging activity. The ethyl acetate fraction and the n-butanol fraction not only showed higher antioxidant activities than the petroleum ether fraction, water fraction and ethanol fraction, but also showed higher superoxide anion and hydrogen peroxide radicals scavenging activities than those of the other extract fractions. The high amount of total phenolics and total flavonoids in the ethyl acetate and n-butanol fractions contributed to their antioxidant activities. The ethyl acetate fraction was subjected to column chromatography, to yield two phenolic compounds, p-hydroxybenzoic acid and vanillic acid, which possessed significant antioxidant activities. Therefore, the seeds of papaya and these compounds might be used as natural antioxidants. PMID:21788927

  10. Compositional changes during papaya fruit ripening

    International Nuclear Information System (INIS)

    The objective of this study was to investigate compositional changes during ripening of Baladi, Ekostika I and Ekostika II papaya fruit cultivars at 20±1°C and 85% -90% relative humidity. The fruits of the three cultivars exhibited a typical climacteric pattern of respiration with peak of respiration of 82,92 and 98 mg CO2/ kg-hr, reached after 10 days in the three cultivars, respectively. Weight loss, total soluble solids (TSS), total sugars and ascorbic acid content progressively increased during ripening of the three papaya cultivars. More increase in TSS and total sugars was observed after the climacteric peak of respiration. Fruit tissue firmness and total phenolic compounds decreased continuously during ripening in the three cultivars. Reducing sugars, total protein and titratable acidity steadily increased to reach a peak, which coincided with climacteric peak of respiration, and subsequently decreased afterwards. The local Baladi cultivar had a lower respiration rate, more firm and less weight loss during ripening, which may indicate a longer shelf life than the other two introduced cultivars. On the other hand, the introduced cultivars were higher in TSS, total and reducing sugars and ascorbic acid content and lower in titratable acidity and phenolic compounds, which may reflect a better eating quality.(Author)

  11. HOMOPTERAN AND MITE PESTS OF PAPAYA AND THEIR CONTROL

    Science.gov (United States)

    Homopteran pests in papaya fields in Florida, include mealybugs, Paracoccus marginatus Williams and Granara de Willink, soft scales Philephedra tuberculosa Nakahara and Gill, Coccus hesperidum L. and armored scales, Aspidiotus destructor, Acutaspis sp., Hemiberlesia sp., aphids, Myzus persicae (Sul...

  12. Effects of chemical treatments on fresh-cut papaya.

    Science.gov (United States)

    Albertini, Silvana; Lai Reyes, Andrés Enrique; Trigo, Juliana Moreno; Sarriés, Gabriel Adrián; Spoto, Marta Helena Fillet

    2016-01-01

    Four treatments (control, 0.1% cinnamaldehyde, 0.75% calcium chloride and combination of 0.1% cinnamaldehyde and 0.75% calcium chloride) were used to evaluate chemical effects on shelf life, quality and sensory acceptability of fresh-cut papaya (Carica papaya L.). Papaya slices were packed and covered with polypropylene film, stored at 5 °C; and evaluated after 1, 3, 6, 9, 12, and 15 days for microbiological and physicochemical changes. A sensory evaluation was performed at 1, 3, 6, 9, and 12 days. There was no occurrence of Salmonella, Escherichia coli or psychotropic bacteria. The cinnamaldehyde alone and a combination of cinnamaldehyde and calcium chloride treatments yielded better control of the total coliforms. The combination treatment decreased the CO2 concentration and increased the maintenance of papaya firmness. All the treatments had acceptability. The combination treatment was the most effective treatment for flavor, taste, and preservation until day 12. PMID:26213093

  13. Influence of thickness on the drying of papaya puree (carica papaya l.) through refractance windowtm technology

    OpenAIRE

    MARÍA U. OCORÓ-ZAMORA; Alfredo A Ayala-Aponte

    2014-01-01

    The aim of this study was to evaluate the influence of sample thickness (2, 3 and 4 mm) on the drying kinetics, water activity (aw) and color (DE) of papaya puree slices processed through Refractance WindowTM technology (RWTM). Additionally, the water diffusion coefficient (Deff) was evaluated taking into account the shrinkage effect. The experimental values of moisture were fitted using Newton's and Midilli’s models. The results showed that the lower the thickness, the faster the drying, the...

  14. Caracterización de accesiones de papaya (Carica papaya L.) a través de marcadores AFLP en Cuba

    OpenAIRE

    Maruchi Alonso Esquivel; Martín Bautista Alor; Matilde Ortiz García; Adriana Quiroz Moreno; Wolfgang Rohde; Lorenzo Felipe Sánchez Teyer

    2010-01-01

    Los marcadores moleculares son herramientas valiosas en los estudios genéticos en plantas, y están siendo empleados exitosamente en programas de mejoramiento principalmente en la elección de progenitores y en la selección. El polimorfismo observado mediante la técnica molecular AFLP (Amplified Fragment Length Polymorphism) ha sido de utilidad para estudios de diversidad genética en frutales. En el presente trabajo se realizó la caracterización molecular de 12 accesiones de papaya (Carica papa...

  15. Monoclonal antibodies to the two most basic papaya proteinases.

    Science.gov (United States)

    Goodenough, P W; Kilshaw, P J; McEwan, F; Owen, A J

    1986-08-01

    The proteinases from Carica papaya include papain, isoenzymes of chymopapain and two proteinases A and B distinguished by their unusually high pI. The identity of one of the most basic proteinases has been questioned. The present report describes the preparation and characterisation of two monoclonal antibodies that react specifically with papaya proteinases A and B respectively and a third that identifies a common structural feature found in papain and proteinase A. PMID:3545314

  16. ESR detection procedure of irradiated papaya containing high water content

    Energy Technology Data Exchange (ETDEWEB)

    Kikuchi, Masahiro, E-mail: kikuchi.masahiro@jaea.go.j [Quantum Beam Science Directorate, Japan Atomic Energy Agency, 1233 Watanuki-machi, Takasaki, Gunma 370-1292 (Japan); Shimoyama, Yuhei [Quantum Beam Science Directorate, Japan Atomic Energy Agency, 1233 Watanuki-machi, Takasaki, Gunma 370-1292 (Japan); Ukai, Mitsuko [Hokkaido University of Education, 1-2 Hachiman-cho, Hakodate, Hokkaido 040-8567 (Japan); Kobayashi, Yasuhiko [Quantum Beam Science Directorate, Japan Atomic Energy Agency, 1233 Watanuki-machi, Takasaki, Gunma 370-1292 (Japan)

    2011-05-15

    ESR signals were recorded from irradiated papaya at liquid nitrogen temperature (77 K), and freeze-dried irradiated papaya at room temperature (295 K). Two side peaks from the flesh at the liquid nitrogen temperature indicated a linear dose response for 3-14 days after the {gamma}-irradiation. The line shapes recorded from the freeze-dried specimens were sharper than those at liquid nitrogen temperature.

  17. Yellow Krang – A New Cultivar Of Papaya For Green Consumption With Tolerance To Papaya Ringspot Virus

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    Janthasri Rapatsa

    2015-12-01

    Full Text Available The development of a papaya cultivar for unripe or green consumption that has resistance to papaya ringspot virus (PRSV began in 2004 by crossing red-fleshed ‘Red Krang’ cultivar with yellow-fleshed Sai Nampeung cultivar. Numerous diverse hybrids that were obtained were selected in a recurrent selection until 2010. The ’Yellow Krang’ line was selected because of the early and high yielding, elongated fruit shape, is yellow and crispy flesh when unripe and suitable for consumption as green papaya, and tolerance to PRSV. From 2010 to 2015 the ‘Yellow Krang’ line was refined through pure line selection. It was evaluated in four growing areas in Thailand for 3 years. The field trial showed that ‘Yellow Krang’ can be classified as dwarf, reaching a height of 110-150 cm, early bearing, with mean 105 days to first flower anthesis; an average of 100 flowers and 206 fruits per plant, mean fruit weight 1.2 kg, mean fruit thickness 2.4 cm, mean fruit firmness 7.15 Newtons; high yielding, with mean fresh weight yield of 134.606 kg/hectare; and 24.86% incidence of PRSV.

  18. Characterization of a high oleic oil extracted from papaya (Carica papaya L. seeds

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    Cassia Roberta Malacrida

    2011-12-01

    Full Text Available The physicochemical characteristics, fatty acid, tocopherol, and carotenoid composition of a crude oil extracted from papaya (Carica papaya L. seeds, formosa variety, were investigated. The oil yield from the seeds was 29.16%. The data obtained for the analytical indexes were in agreement with those of other edible oils. The oil obtained had high oxidation resistance (77.97 hours. The major fatty acids in total lipid were oleic (71.30%, palmitic (16.16%, linoleic (6.06%, and stearic (4.73% acid. The ? and ?-tocopherol were the predominant tocopherols with 51.85 and 18.89 mg.kg-1, respectivelly. The ?-cryptoxanthin (4.29 mg.kg-1 and ?-carotene (2.76 mg.kg-1 were the carotenoids quantified, and the content of total phenolic compounds was 957.60 mg.kg-1. Therefore, the potential utilization of the papaya seeds for oil production seems favorable. However, toxicological studies need to be carried out before the oil is appropriate for food applications.

  19. The Extraction and Properties of Carica papaya Seed Oil

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    Y.M. Li

    2015-04-01

    Full Text Available The main objective of the present study was to evaluate the suitability of Ultrasound-Microwave synergistic Extraction (UMAE for the recovery of papaya seed oil as compared to Ultrasound-Assisted Extraction (UAE. The efficiency of these two methods was assessed by comparing the physicochemical properties and oxidative stability of papaya seed oil. The analytic tests were color, unsaponifiable matters, iodine value, acid value and peroxide value. The fatty acid components were analyzed by GC. Results indicated that the Ultrasound-Microwave synergistic Extraction (UMAE considerably impact on the physicochemical properties of the extracted papaya seed oil, UMAE provided papaya seed oil with obviously lighter color, lower unsaponifiable matters (2.53% and higher oxidative stability (PV, 0.98 m mol/kg than that of UAE. Analysis of fatty acid composition revealed that 13 kinds of components are identified and the total amount of fatty acids accounted for 93.13% of papaya seed oil, the predominant fatty acids in papaya seed oil were oleic (18:1, 72.60%, palmitic (16:0, 18.00%, linoleic acid (18:2, 5.80% and stearic (18:0, 3.60%.

  20. Efficacy of four plant extracts on nematodes associated with papaya in Sindh, Pakistan

    Science.gov (United States)

    This investigation examines the effect of ethanol extracts of four plant species--Azadirachta indica (neem), Withania somnifera (ashwagandha), Tagetes erecta (marigold) and Eucalyptus citriodora (eucalyptus)--against nematodes associated with papaya (Carica papaya), and it assesses their influence o...

  1. COMUNEROS ANDINOS EN LA ADMINISTRACIÓN CHILENA (ARICA, 1880-1929

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    ALBERTO DÍAZ ARAYA

    2011-05-01

    Full Text Available Resumen Se evalúa el impacto generado en los sectores rurales andinos de Arica por la implementación de la estructura y burocracia administrativa chilena post Guerra del Pacífico. A través de registros documentales se hace explícita la participación de comuneros indígenas en la trama estatal chilena en un período de conflicto político y de identidades nacionales. Palabras claves: Comunidad andina – administración chilena – Arica y Tacna. Abstract Assesses the impact generated in the rural Andes Arica for implementing the administrative structure and bureaucracy in Chile after the Pacific War. Through documentary record makes explicit the participation of indigenous community members in the plot Chilean state over a period of political and national identities. Keywords : Andean Community - Chilean government - Arica and Tacna.

  2. Effects of Different Concentrations and Applications of Calcium on Storage Life and Physicochemical Characteristics of Papaya (Carica Papaya L.

    Directory of Open Access Journals (Sweden)

    T. M. M. Mahmud

    2008-01-01

    Full Text Available Papaya (Carica Papaya L. fruits index 2 were treated with 1.5, 2.5 and 3.5% solutions of calcium chloride by dipping and vacuum infiltration (-33 Kpa or untreated (0% as control. Effects of these treatments were evaluated on storage life and postharvest quality characteristics of papaya. After 21 days of storage at 13±1°C, the fruits were removed from storage for physicochemical analysis. Following additional five days holding in the storage condition for fruits used for evaluation of the rate of disease incidence and storage life. Postharvest dip treatments at different concentrations of calcium prolonged storage life, slowed down the ripening processes and maintained the quality of papaya. Whereas, it was effectively greater with calcium infiltration treatments than that for dip treatments. Calcium infiltration extended the storage life and retained the quality as calcium concentrations increased up to 2.5% and then declined. The desired effect was obtained at 2.5% infiltration compared with other treatments. The least disease incidence was found in those fruits infiltrated with 2.5% calcium. Hence, it can be concluded that postharvest infiltration of calcium at 2.5% has the potential to control disease incidence, prolong the storage life and preserve valuable attributes of postharvest papaya, presumably because of its effects on inhibition of ripening and senescence process and loss of the fruit firmness of papaya.

  3. La izquierda chilena contemparánea

    Directory of Open Access Journals (Sweden)

    Manuel Antonio Garretón

    2006-01-01

    Full Text Available En este artículo, el autor examina las particularidades de la izquierda chilena para entender su papel en los gobiernos democráticos desde los años 70. El autor parte de la premisa de la existencia, durante los años 90, de dos izquierdas: una, el socialismo dentro de la coalición; la otra, el comunismo fuera de ella. En el trabajo se sostiene que la izquierda socialista ha sido el principal motor de la coalición y su papel específico se ha concentrado en los temas socio-económicos ligados a la igualdad, a la justicia, a los derechos humanos y a las propuestas culturales de tipo progresista. Asimismo, se analizan las particularidades esenciales de los gobiernos democráticos chilenos y se examinan los temas y las tareas pendientes. El artículo concluye con un balance del papel de la izquierda y de los retos que aún tiene por delante.

  4. ESTIMACIÓN DEL ÁREA FOLIAR DE PAPAYA (Carica papaya L. BASADA EN MUESTREO NO DESTRUCTIVO ESTIMATION OF PAPAYA (Carica papaya L. LEAF AREA BASED ON NON-DESTRUCTIVE SAMPLING

    Directory of Open Access Journals (Sweden)

    Carlos Cardona Ayala

    Full Text Available El cultivo de papaya (Carica papaya L. es muy importante en la fruticultura del departamento de Córdoba, por la buena calidad de la fruta producida. La investigación, se realizó en Montería, a 13msnm, temperatura media de 27.4°C, precipitación anual de 1346,1mm, humedad relativa de 84% y brillo solar anual de 2180,2 horas, con el fin de obtener un modelo que permita estimar el área foliar de la planta, a partir de muestreo no destructivo. Mediante muestreo aleatorio simple, se seleccionaron hojas sanas de plantas adultas, de diferentes tamaños y distintas etapas de desarrollo, de los doseles superior, medio e inferior, de la variedad Maradol roja. Se tomaron medidas longitudinales: largo y ancho máximo de la hoja, ancho de la hoja a un medio, un tercio, un cuarto, un quinto y dos tercios de su largo, ancho máximo de lóbulos y longitud de nervadura de los mismos, con la finalidad de determinar las medidas altamente relacionadas con el área fotosintética de la hoja. El área foliar (Y, se midió por el método de relación peso: área, sobre una muestra de 30 hojas por dosel. Se construyeron modelos de regresión simple y múltiple, para luego seleccionar el de mejor ajuste. Se seleccionó un modelo de regresión simple, cuya variable explicativa corresponde a la longitud de la nervadura central del lóbulo izquierdo, contiguo al adyacente lóbulo medio (X. El modelo ajustado resultó ser: o = -303,0742 + 31,2028X, R² = 0,9335.The papaya (Carica papaya L is a very important crop in the Cordoba state, especially for the good fruit quality. This research was carried out at Montería at 13masl, 27.4°C, annual rainfall of 1346.1mm, 84% relative humidity and 2180.2 hours annual sunlight, in order to obtain a model to estimate the plants leaf area, based on non-destructive sampling. A probability sampling procedure was used to select different size leaves and developmental stages from the upper, middle and lower canopy of adult plants of the red Maradol variety. Maximum leaf length and width, width of the middle third, fourth, fifth and two-thirds of leaf length, lobe width and midrib length of every lobe were registered with the purpose of identifying measures highly related to photosynthetic leaf area. The leaf area (Y was measured based on the weight:area relation, from 30 leaves per canopy sample. Simple linear regression models and multiple linear regression were adjusted. A simple linear regression model was selected which independent variable representing the length of the midrib, next to the left next lobe and adjacent to the middle lobe (X. The adjusted model was: or = -303,0742 + 31,2028X, R² = 0,9335.

  5. THERAPEUTIC POTENTIAL OF CARICA PAPAYA L. LEAF EXTRACT IN TREATMENT OF DENGUE PATIENTS

    OpenAIRE

    CVM Naresh Kumar; V Taranath; A Venkatamuni; Vishnu Vardhan, R.; Y Siva Prasad; U Ravi; DVR Sai Gopal

    2015-01-01

    Carica papaya leaf extracts are known to have many beneficial medicinal properties. Recent studies have claimed possible beneficial effects of Carica papaya leaf extracts in treating patients with dengue infection. The present study aims to evaluate the therapeutic potential of Carica papaya leaf extracts in treating dengue patients. Carica papaya leaf extracts were given to dengue infected patients and their blood samples were daily monitored. Polyserositis condition of the patients was anal...

  6. Origin and domestication of papaya Yh chromosome.

    Science.gov (United States)

    VanBuren, Robert; Zeng, Fanchang; Chen, Cuixia; Zhang, Jisen; Wai, Ching Man; Han, Jennifer; Aryal, Rishi; Gschwend, Andrea R; Wang, Jianping; Na, Jong-Kuk; Huang, Lixian; Zhang, Lingmao; Miao, Wenjing; Gou, Jiqing; Arro, Jie; Guyot, Romain; Moore, Richard C; Wang, Ming-Li; Zee, Francis; Charlesworth, Deborah; Moore, Paul H; Yu, Qingyi; Ming, Ray

    2015-04-01

    Sex in papaya is controlled by a pair of nascent sex chromosomes. Females are XX, and two slightly different Y chromosomes distinguish males (XY) and hermaphrodites (XY(h)). The hermaphrodite-specific region of the Y(h) chromosome (HSY) and its X chromosome counterpart were sequenced and analyzed previously. We now report the sequence of the entire male-specific region of the Y (MSY). We used a BAC-by-BAC approach to sequence the MSY and resequence the Y regions of 24 wild males and the Y(h) regions of 12 cultivated hermaphrodites. The MSY and HSY regions have highly similar gene content and structure, and only 0.4% sequence divergence. The MSY sequences from wild males include three distinct haplotypes, associated with the populations' geographic locations, but gene flow is detected for other genomic regions. The Y(h) sequence is highly similar to one Y haplotype (MSY3) found only in wild dioecious populations from the north Pacific region of Costa Rica. The low MSY3-Y(h) divergence supports the hypothesis that hermaphrodite papaya is a product of human domestication. We estimate that Y(h) arose only ? 4000 yr ago, well after crop plant domestication in Mesoamerica >6200 yr ago but coinciding with the rise of the Maya civilization. The Y(h) chromosome has lower nucleotide diversity than the Y, or the genome regions that are not fully sex-linked, consistent with a domestication bottleneck. The identification of the ancestral MSY3 haplotype will expedite investigation of the mutation leading to the domestication of the hermaphrodite Y(h) chromosome. In turn, this mutation should identify the gene that was affected by the carpel-suppressing mutation that was involved in the evolution of males. PMID:25762551

  7. Protection and conservation of Caricaceae germplasm with PRSV resistant transgenic papaya

    Science.gov (United States)

    Papaya ringspot virus (PRSV) is a devastating disease that has a detrimental impact on both commercial papaya production and Caricaceae germplasm conservation. The PRSV coat protein transgenic line 55-1 and derived progeny are resistant to PRSV and have saved the papaya industry in Hawaii. Here we ...

  8. 7 CFR 319.56-25 - Papayas from Central America and Brazil.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 5 2010-01-01 2010-01-01 false Papayas from Central America and Brazil. 319.56-25... § 319.56-25 Papayas from Central America and Brazil. The Solo type of papaya may be imported into the... shipment to the United States in one of the following locations: (1) Brazil: State of Espirito Santo;...

  9. Application of a qualitative and quantitative real-time polymerase chain reaction method for detecting genetically modified papaya line 55-1 in papaya products.

    Science.gov (United States)

    Nakamura, Kosuke; Akiyama, Hiroshi; Takahashi, Yuki; Kobayashi, Tomoko; Noguchi, Akio; Ohmori, Kiyomi; Kasahara, Masaki; Kitta, Kazumi; Nakazawa, Hiroyuki; Kondo, Kazunari; Teshima, Reiko

    2013-01-15

    Genetically modified (GM) papaya (Carica papaya L.) line 55-1 (55-1), which is resistant to papaya ringspot virus infection, has been marketed internationally. Many countries have mandatory labeling regulations for GM foods, and there is a need for specific methods for detecting 55-1. Here, an event- and construct-specific real-time polymerase chain reaction (PCR) method was developed for detecting 55-1 in papaya products. Quantitative detection was possible for fresh papaya fruit up to dilutions of 0.001% and 0.01% (weight per weight [w/w]) for homozygous SunUp and heterozygous Rainbow cultivars, respectively, in non-GM papaya. The limit of detection and quantification was as low as 250 copies of the haploid genome according to a standard reference plasmid. The method was applicable to qualitative detection of 55-1 in eight types of processed products (canned papaya, pickled papaya, dried fruit, papaya-leaf tea, jam, puree, juice, and frozen dessert) containing papaya as a main ingredient. PMID:23122142

  10. Herbicidas para el control de Spermacoce tenuior L en papaya (Carica papaya)

    Scientific Electronic Library Online (English)

    Antonio, Bogantes-Arias; Moisés, Hernández-Cháves; Eric, Mora-Newcomer.

    2010-06-01

    Full Text Available Herbicidas para el control de Spermacoce tenuior L. en papaya (Carica papaya). Con el objetivo de determinar la eficacia de varios herbicidas en el control de Spermacoce tenuior y otras arvenses en papa ya, se realizó un ensayo en el 2007, en una plantación de papa ya "Pococí" ubicada en Guápiles, e [...] n el Caribe de Costa Rica. La primera etapa consistió en la evaluación de seis tratamientos posemergentes (1. Glifosato 24 SL, 2. Glifosato 68 SG , 3. Glifosato 24 SL + carfentrazone 24 EC , 4. Glifosato 68 SG + carfentrazone 24 EC , 5. Glifosato 24 SL + metsulfurón metil 60 WG, 6. Glifosato 24 SL + oxifluorfen 24 EC). En la segunda etapa se evaluaron tres preemergentes (1. Orizalina 75 WP, 2. Oxifluorfen 12 EC, 3. Acetaclor 90 EC); distribuidos en bloques al azar. Se evaluó la cobertura de especie s antes y después de la aplicación, daño al cultivo, altura y grosor de plantas de papa ya. El tratamiento posemergente glifosato (0,94 kg i.a./ ha) + metsulfurón (16 g i.a./ha) en aplicación dirigida, mostró el mejor control de malezas a partir de la segunda y hasta la sexta semana después de la aplicación (SDA), sin observarse daño en el cultivo. El herbicida preemergente orizalina (3 kg i.a./ha) mostró el mejor control de Spermacoce tenuior hasta las 14 SDA tanto en el lomillo como en la rodaja. No se observó fitotoxidad en el cultivo. Abstract in english Herbicide perfomance for Spermacoce tenuior L. control in papaya (Carica papaya). In order to assess the effectiveness of pre and post-emergence herbicides for the control of Spermacoce tenuior and other weeds in papaya, a field test was conducted in 2007, in a commercial field of the "Pococí" hybri [...] d, located in Guápiles, Limón, Costa Rica. A first phase of the study consisted of six post-emergent treatments (1. Glyphosate 24 SL, 2. Glyphosate 68 SG , 3. Glip hosate 24 SL + carfentrazone 24 EC , 4. Glyphosate 68 SG + carfentrazone 24 EC , 5. Glip hosate 24 SL + metsulfuron methyl 60 WG, 6. Gliphosate 24 SL + oxyfluorfen 24 EC). A second phase consisted of three pre-emergent treatments (1. Oryzalin 75 WP, 2. Oxyfluorfen 12 EC, 3. Acetoclor 90 EC) distributed in a randomized complete block design in both phases. The percentage of weed ground cover, height and stem thickness of papaya plants were measured before and after herbicide app lication. Post-emergence applications of glyphosate (0.94 kg i.a./ha) + metsulfuron (16 g i.a./ha) showed the best weed control from the second until the sixth week after application (WAA ) without affecting the crop. Oryzalin applied as a preemergent (3 kg i.a./ha) showed the best control of Spermacoce tenuior until 14 WAA under the planting row and around the plants. No phytotoxicity was observed.

  11. Escarificación química de semilla de papaya / Chemical scarification of papaya seed

    Scientific Electronic Library Online (English)

    Jorge Arturo, Romero Rodríguez; José Apolinar, Mejía Contreras; Aquiles, Carballo Carballo; Alfredo, López Jiménez; José Antonio, Rangel Lucio; Catarino, Ávila Reséndiz.

    2013-09-01

    Full Text Available La germinación tardía y errática de semilla de papaya es afectada por la presencia de la sarcotesta, membrana que contiene compuestos fenólicos inductores de latencia, misma que inhibe el intercambio de líquidos y gases, prolongar el período de secado y facilitar la colonización de fitopatógenos. La [...] s técnicas utilizadas en el beneficio de semilla de papaya para eliminar la sarcotesta son limitadas, por tanto, el objetivo del presente estudio fue obtener tecnología útil en éste aspecto, para lo cual se comparó tratamientos a base de hidróxido de sodio, ácido sulfúrico y clorhídrico, y evaluó su efecto en la calidad física, sanitaria y fisiológica de la semilla. La investigación se realizó en el Laboratorio de Análisis de Semillas del Colegio de Postgraduados, Campus Montecillo, Estado de México, en 2011. El NaOH al 25 % y 15 min de inmersión, eliminó 98% de sarcotesta, presentó sólo 2% de incidencia de micoflora, incremento y homogeneizó la germinación. El H2SO4 eliminó al 100% la sarcotesta, inhibió la colonización de hongos con sólo 6%, cuando se empleó en forma concentrada, pero afecto negativamente la germinación. El HCl fue ineficiente en eliminar la sarcotesta (9.6%), presentó la más alta colonización de hongos (97% de incidencia) y el menor porcentaje de germinación (7%). Los efectos positivos del uso de NaOH en el beneficio de semilla de papaya, simula la degradación natural de la sarcotesta y mejora la condición de la semilla por lo que resulta una alternativa viable para su empleo en el acondicionamiento de semilla. Abstract in english Late germinating and erratic seed from papaya is affected by the presence of sarcotesta, membrane containing phenolic compounds inducing latency same which inhibits the exchange of liquids and gases, prolongs the drying period and facilitates colonization of pathogens. The techniques used in benefit [...] of the papaya seed to eliminate the sarcotesta are limited, therefore the aim of this study was to obtain useful technology in this aspect, which was compared to other treatments with sodium hydroxide, sulfuric acid and hydrochloric acid and assessed its effect on the physical, health and physiological quality of the seed. The research was conducted at the Seed Testing Laboratory of the Graduate College, Campus Montecillo, State of Mexico, in 2011. NaOH to 25% and 15 min of immersion, removed 98% of sarcotesta, presented just 2% of incidence of micoflora, an increased and homogenized germination. H2SO4 to 100% removed the sarcotesta, inhibited fungal colonization with only 6% when used in concentrated form, but negatively affected the germination. The HCl was ineffective in eliminating sarcotesta (9.6%) had the highest fungal colonization (97% incidence) and the lowest germination percentage (7%). The positive effects of the use of NaOH in the benefit of papaya seed, is that simulates natural degradation of sarcotesta and improves the condition of the seed, so it is a viable alternative for use in conditioning seed.

  12. Una taxonomía de las universidades chilenas

    Scientific Electronic Library Online (English)

    Miguel, Muñoz; Christian, Blanco.

    2013-06-01

    Full Text Available Se propone una taxonomía de las universidades chilenas mediante análisis factorial, que resulta distinta a las tipologías de Brunner (2005, 2009), Parada (2010), Torres y Zenteno (2011) y Rosso y Reyes (2012), y supera las objeciones de Bernasconi (2006). Evita, además, distinciones binarias del tip [...] o tradicional/privada, investigación/docencia, metropolitana/regional, católica/laica, etc. Tampoco establece rankings ni utiliza condiciones históricas, legales u otras alejadas de los modos de operación efectivos de las universidades. La siguiente taxonomía las agrupa según un pool de variables relevantes (investigación, tamaño, composición, acreditación, etc.) que da pie a cinco tipos de universidades: investigación, masivas, de acreditación, elitistas y no elitistas. Se espera aportar en la comprensión de las universidades y contribuir a las políticas públicas con herramientas conceptuales para abordar la diversidad universitaria. Abstract in english We propose a taxonomy of Chilean universities through a factor analysis with different results from those produced by typologies of Brunner (2005, 2009), Parada (2010), Torres and Zenteno (2011) and Rosso and Reyes (2012), overcoming Bernasconi’s objections (2006). It avoids binary distinctions like [...] traditional/private, metropolitan/regional, catholic/secular, etc. It does not establish rankings nor use historical, legal or other distinctions not referred to the effective universities’ operational characteristics. Universities are grouped according to a pool of relevant variables (research, size, composition, accreditation, etc.). Five types of universities are generated: Research, Massive, Accreditation, Elite and Non-Elite. This research intends to contribute to the understanding of universities in Chile and to public policies with conceptual tools for addressing the diversity of universities.

  13. NEMATODOS FITOPARASITOS ASOCIADOS AL CULTIVO DE PAPAYA (Carica papaya L. EN CÓRDOBA

    Directory of Open Access Journals (Sweden)

    Manuel R. Espinosa

    2004-01-01

    Full Text Available El presente trabajo tuvo como objetivo identificar los nematodos fitoparasitos asociados al cultivo de papaya ( Carica papaya L en el departamento de Córdoba y correlacionar las características edafológicas con la presencia de los mismos. Se tomaron muestras de raíces y suelo rizosférico en los municipios de Tierralta, Valencia, Montelíbano, Montería y Lorica, de las que se obtuvieron los diferentes estadíos. De las muestras de suelo se evaluaron el pH, CE, MO y textura. Se evaluaron variables morfológicas y morfométricas para determinar los géneros y las especies. Se encontraron los géneros Paratylenchus , Xiphinema, Helicotylenchus, Pratylenchus, Tylenchus, Ditylenchus, Trophurus, Aphelenchus, Psilenchus, Tylenchorhynchus, Criconemella, Hoplolaimus y Meloidogyne , con las especies; Meloidogyne javanica , M. incognita y M. arenaria. El pH de los suelos varió de 4.86 a 6.9; la CE de 0.097 a 1.4 dS m- 1; la MO de 1.2 a 3.1% y la textura del suelo fue arenosa, franca y franco arenosa. Las arenas oscilan del 45 al 86%, arcilla del 3 al 22% y limo del 10.9 al 47%. El análisis de correlación mostró que el contenido de arena esta correlacionado positivamente; mientras que el pH, la CE y la MO están correlacionados negativamente con la riqueza y el número de individuos de los nematodos fitoparasitos en el departamento de Córdoba. Los resultados permiten el reporte por primera vez de M. incognita, M javanica y M. arenaria afectando conjuntamente el cultivo de papaya en la Costa Caribe Colombiana.

  14. Evaluación de marcadores genéticos para discriminación entre hembras y hermafroditas de papaya (Carica papaya L.) variedad 'Maradol' / Evaluation of genetic markers for discrimination between females and hermaphrodites of papaya (Carica papaya L.) CV. 'Maradol'

    Scientific Electronic Library Online (English)

    Violeta, Aspeitia-Echegaray; Ma. Alejandra, Torres-Tapia; Dulce V., Mendoza-Rodríguez; M. Humberto, Reyes-Valdés.

    2014-09-01

    Full Text Available La papaya (Carica papaya L.) presenta tres tipos sexuales: macho, hembra y hermafrodita, de los cuales solo el último posee valor comercial y calidad de exportación. Con base en su morfología, dichos tipos únicamente pueden ser identificados a partir de la floración. La segregación del sexo en esta [...] especie se explica con un modelo de un locus multialélico, aunque tiene una base molecular más compleja ya que puede intervenir más de un gen. En la papaya 'Maradol' se presentan casi exclusivamente los tipos femenino y hermafrodita, por lo cual en esta variedad se busca solamente la distinción entre ambas formas sexuales. En este trabajo se probaron tres juegos de iniciadores para marcadores SCAR, previamente desarrollados para determinación del sexo en variedades hawaianas. Los tres marcadores se identifican como T1, T12 y W11. A partir de tejido de 17 plantas identificadas como hembras y 23 hermafroditas de papaya 'Maradol' se encontró que los marcadores T12 y W11 fueron específicos en 100 % para plantas hermafroditas, mientras que no se observó amplificación para las plantas hembra. el SCAR T1 amplificó ADN solamente en algunas plantas hermafroditas. Se concluye que los SCAR T12 y W11 pueden ser utilizados como parte de una técnica para identificación temprana del sexo de las plantas de papaya 'Maradol' con fines de plantación comercial, previa validación con otras poblaciones de la misma variedad. Abstract in english Papaya (Carica papaya L.) shows three sexual types: male, female and hermaphrodite, from which only the last one has commercial value and export quality. Morphologically, such types are only distinguishable starting at flowering stage. Sex segregation in this species is explained by a multiallelic l [...] ocus, even though it is more complex at the molecular level. In 'Maradol' papaya, the female and hermaphrodite types are almost the only present sexual forms, so that the distinction between these two forms is sought. In this work, three sets of SCAR primers, previously developed for Hawaiian varieties, were tested. The three markers are identified as T1, T12 and W11. From tissue of 17 female and 23 hermaphrodite plants, it was found that the markers T12 and W11 showed 100% specificity for hermaphrodite individuals, with null amplification for female plants. On the other hand, the T1 SCAR primers amplified DNA only in some hermaphrodite plants. It is concluded that T12 and W11 SCAR markers can be used as a part of a technique to identify plant sex in early stages of 'Maradol' papaya for commercial cultivation purpose, after validation with other populations of the same variety.

  15. Effect of ozone on anthracnose physicochemical responses and gene expression in papaya (carica papaya l.)

    OpenAIRE

    Ong, Mei Kying

    2014-01-01

    A study was conducted to investigate the effects of varying levels of ozone (0, 1.5, 2.5, 3.5 or 5.0 ppm) for 96 h on 1. the in vitro and in vivo growth of Colletotrichum gloeosporioides, the causal organism of anthracnose; 2. the reactive oxygen species generation and spore mitochondria of C. gloeosporioides using transmission electron microscope, fluorescence microscope and laser scanning confocal microscope; 3. the production of defence-related enzymes in papaya; 4. microbiological analysi...

  16. Detection method for genetically modified papaya using duplex PCR.

    Science.gov (United States)

    Yamaguchi, Akihiro; Shimizu, Kaori; Mishima, Takashi; Aoki, Nobutaro; Hattori, Hideki; Sato, Hidetaka; Ueda, Nobuo; Watanabe, Takahiro; Hino, Akihiro; Akiyama, Hiroshi; Maitani, Tamio

    2006-08-01

    A simple and rapid method for the identification of genetically modified (GM) papaya, derived from Line 55-1, was developed by modifying the Japanese official PCR method. Genomic DNA was directly extracted from the fresh fruit without the lyophilization step, using a commercial silica-based kit. To develop a duplex PCR method which simultaneously detects the GM papaya-specific gene and the intrinsic papain gene, the papain 2-5'/3' (amplicon size; 184 bp) primer pair for the detection of the papain gene was newly designed within the region of the products (211 bp) amplified using the papain 1-5'/-3' primer pair adopted in the Japanese official PCR method. To detect the GM papaya-specific gene, the primer pair Nos C-5'/CaM N-3' described in the Japanese official method was used. The DNA sequences of the GM papaya gene and the intrinsic papain gene were co-amplified using the PCR method in a single tube. The developed duplex PCR method allows the simultaneous detection of the products by means of agarose gel electrophoresis or microchip electrophoresis. The proposed method for GM papaya identification is simple and rapid. PMID:16984033

  17. Effect of gamma-irradiation on ripening papaya pectin

    International Nuclear Information System (INIS)

    Papaya (Carica papaya, L., var. Sunset) at three initial ripeness stages were irradiated with 0.25, 0.50, 0.75, 1.0, or 1.5 kGy gamma-irradiation and pectin changes during ripening determined. A significant linear relationship was found between irradiation dose and firmness immediately after irradiation. Irradiation had no effect on fruit skin or flesh color of papaya fruit irradiated at the 5 to 30% yellow stage and allowed to ripen. Papaya irradiated when 5 to 30% yellow showed no significant changes in pectin methylesterase activity when ripe. Immediately after irradiation, the pectin in 10 to 30% yellow papaya showed depolymerization and demethoxylation, though no effect on pectin methylesterase activity was detected. There was an increase in water soluble pectin (WSP), while chelator soluble (CSP) and alkali soluble pectin (ASP) decreased, with a significant decline in the methanol content of the ASP fraction. After the 25 to 30% yellow ripeness stage, fruit irradiated at 0.50 to 1.0 kGy had less pectic depolymerization, and had a firmer texture than nonirradiated when ripe. A lower level of WSP and higher levels of CSP and ASP were found in ripe fruit that had been irradiated at 0.5 to 1.0 kGy when 25 to 30% yellow skin with a significant quadratic relationship between irradiation dose and the three pectin fractions. The firmness of these irradiated fruit were retained for two days longer than the nonirradiated control. (author)

  18. OBTAINING ACTIVATED CARBON FROM PAPAYA SEEDS FOR ENERGY STORAGE DEVICES

    Directory of Open Access Journals (Sweden)

    Ariharaputhiran Anitha

    2015-01-01

    Full Text Available Preparation of carbon from papaya (Carica papaya L. seeds activated with ZnCl2 @ 300°C is presented. Characterization of the powder is carried out by elemental analysis, N2 sorption studies, powder x-ray diffractometry, fourier transform infrared spectroscopy, scanning electron microscopy, cyclic voltammetry and galvanostatic charge-discharge studies. Specific capacitance from cyclic voltammetric studies on the carbon is 472 F g-1 in 1M H2SO4 electrolyte when run between -0.1 and 1.0V Vs SCE at 10 mV s-1 scan rate. The proposed carbon sample posses an N2 adsorption isotherm characteristic of mesopore structures. The presence of various surface functional groups like carbonyl and hydroxyl groups coupled with desired physical features of the carbon are presumed to be responsible for fast faradic reactions to offer pseudocapacitive characteristics to the papaya derived carbon. Capacity as well as the cycling stability of the electrode prepared with papaya seed based activated carbon in acid electrolyte seems to be impressive as observed from charge-discharge studies. Thus papaya seed biomass represents a promising new green source of carbon for electrochemical capacitor electrodes and gives a new and valuable dimension to domestic wastes.

  19. INFLUENCIA DE LA OSMOCONGELACIÓN SOBRE ALGUNAS PROPIEDADES FÍSICAS DE PAPAYA (Carica papaya L.) / INFLUENCE OF OSMODEHYDROFREEZING ON SOME PHYSICAL PROPERTIES OF PAPAYA (Carica papaya L.)

    Scientific Electronic Library Online (English)

    Alfredo, Ayala Aponte; Martha, Sánchez; Hanny, Rodríguez.

    2014-12-01

    Full Text Available La osmo-congelación (OC) es el proceso combinado de aplicar la deshidratación osmótica (DO) seguido de la congelación en un alimento, para mejorar la calidad del producto congelado-descongelado. El objetivo de este trabajo fue evaluar la influencia de la aplicación de pretratamientos osmóticos sobre [...] la pérdida de fase líquida (PFL), volumen (V) y color, en términos de claridad (L*), tono (h°) y cambio total de color (?E), en muestras de papaya, almacenadas en congelación. Las muestras fueron deshidratadas osmóticamente hasta 30 y 90 min, alcanzando, respectivamente, contenidos de humedad, de 81,40±0,69% y 76,24±0,41%(b.h); para ello, se empleó una solución osmótica (SO) de sacarosa, con 65°Brix, a 26,0+0,2°C. Las muestras deshidratadas osmóticamente fueron congeladas a -40°C y, posteriormente, se almacenaron a -18°C, durante 10, 20, 30 y 40 días. Como tratamiento control, se utilizaron muestras no tratadas osmóticamente (MNT), durante el almacenamiento en congelación. Los resultados mostraron que, en todos los tratamientos, el tiempo de almacenamiento en congelación influyó significativamente (p Abstract in english The osmo-dehydro-freezing (OC) is the combined process of applying osmotic dehydration (OD) followed by freezing of a food product to improve the quality of frozen-thawed products. The aim of this study was to evaluate the influence of the application of osmotic pretreatments on the loss of liquid p [...] hase (PFL), volume (V) and color in terms of lightness (L*), hue (h°), and color change (?E) of papaya frozen stores samples. The osmotically dehydrated samples were treated during 30 and 90 min, reaching moisture content of 81,40±0,69 and 76,24±0,41% (wb) respectively. For this, an osmotic solution of sucrose with 65°Brix at 26.0°C+0.2 was used. The treated samples were frozen at -40°C and subsequently stored at -18°C for 10, 20, 30 and 40 days. As control osmotically untreated samples (MNT) during frozen storage were used. The results showed that in all treatments the frozen storage time significantly influenced (p

  20. Physico-chemical characteristics of papaya (Carica papaya L.) seed oil of the Hong Kong/Sekaki variety.

    Science.gov (United States)

    Yanty, Noorzianna Abdul Manaf; Marikkar, Jalaldeen Mohammed Nazrim; Nusantoro, Bangun Prajanto; Long, Kamariah; Ghazali, Hasanah Mohd

    2014-01-01

    A study was carried out to determine the physicochemical characteristics of the oil derived from papaya seeds of the Hong Kong/Sekaki variety. Proximate analysis showed that seeds of the Hong Kong/Sekaki variety contained considerable amount of oil (27.0%). The iodine value, saponification value, unsaponifiable matter and free fatty acid contents of freshly extracted papaya seed oil were 76.9 g I2/100g oil, 193.5 mg KOH/g oil, 1.52% and 0.91%, respectively. The oil had a Lovibond color index of 15.2Y + 5.2B. Papaya seed oil contained ten detectable fatty acids, of which 78.33% were unsaturated. Oleic (73.5%) acid was the dominant fatty acids followed by palmitic acid (15.8%). Based on the high performance liquid chromatography (HPLC) analysis, seven species of triacylglycerols (TAGs) were detected. The predominant TAGs of papaya seed oil were OOO (40.4%), POO (29.1%) and SOO (9.9%) where O, P, and S denote oleic, palmitic and stearic acids, respectively. Thermal analysis by differential scanning calorimetry (DSC) showed that papaya seed oil had its major melting and crystallization transitions at 12.4°C and -48.2°C, respectively. Analysis of the sample by Z-nose (electronic nose) instrument showed that the sample had a high level of volatile compounds. PMID:25174674

  1. An Analysis on DNA Fingerprints of Thirty Papaya Cultivars (Carica papaya L.), Grown in Thailand with the Use of Amplified Fragment Length Polymorphisms Technique

    OpenAIRE

    Janthasri Ratchadaporn; Katengam Sureeporn; U. Khumcha

    2007-01-01

    The experiment was carried out at the Department of Horticulture, Ubon Ratchathani University, Ubon Ratchathani province, Northeast Thailand during June 2002 to May 2003 aims to identify DNA fingerprints of thirty papaya cultivars with the use of Amplified Fragment Length Polymorphisms (AFLP) technique. Papaya cultivars were collected from six different research centers in Thailand. Papaya plants of each cultivar were grown under field conditions up to four months then leaf numbers 2 and 3 of...

  2. INFLUENCE OF THICKNESS ON THE DRYING OF PAPAYA PUREE (Carica papaya L. THROUGH REFRACTANCE WINDOWTM TECHNOLOGY

    Directory of Open Access Journals (Sweden)

    MARÍA U. OCORÓ-ZAMORA

    2013-01-01

    Full Text Available El objetivo de este trabajo fue evaluar la influencia del espesor de las muestras (2, 3 y 4 mm sobre las cinéticas de secado, actividad de agua (aw y color (?E de rodajas de puré de papaya procesadas por tecnología de ventana de refractanciaTM (RWTM. Adicionalmente se evaluó la difusividad del agua (Deff teniendo en cuenta el encogimiento. Los datos de humedad se ajustaron mediante los modelos de Newton y Midilli. Los resultados mostraron que a menor espesor el secado fue más rápido, los valores de aw fueron menores y los ?E superiores. Las muestras alcanzaron 0.0652, 0.1132 y 0.2624 g agua/ g sólido seco en 60 min para rodajas de 2, 3 y 4 mm, respectivamente. El Modelo de Midilli fue el más apropiado para predecir las curvas de secado de papaya por RWTM. Deff disminuyó a menor espesor y su orden de magnitud fue de 10-10 m2/s.

  3. Sequence and genome organization of papaya meleira virus infecting papaya in Brazil.

    Science.gov (United States)

    Abreu, Emanuel F M; Daltro, Cleidiane B; Nogueira, Elsa O P L; Andrade, Eduardo C; Aragão, Francisco J L

    2015-12-01

    Papaya sticky disease ('meleira') was first observed in Brazil at the beginning of the 1980s. The disease is characterized by intense latex exudation from the fruit surface that becomes dark as it oxidizes, which makes it difficult to sell. The causal agent, which has been called papaya meleira virus (PMeV), has been identified as an isometric virus particle, approximately 50 nm in diameter, with a double-stranded RNA genome. Here, we report the first complete sequence and organization of the 8.7-kb viral dsRNA genome. Two ORFs coding for a putative coat protein and RNA-dependent RNA polymerase (RdRp) were predicted. In silico analysis revealed that the translated ORF2 contains the conserved domains characteristic of an RdRp protein (pfam02123:RdRP 4), which is a family that includes RdRps from members of the genera Luteovirus, Totivirus and Rotavirus. Evolutionary analysis with amino acid sequences with the RdRps from members of the family Totiviridae and some dsRNA viruses showed that PMeV RdRp did not root itself in any genus. PMID:26370790

  4. Consumer in-store response to irradiated papayas

    International Nuclear Information System (INIS)

    In this study, purchase behavior of California consumers in response to irradiated papayas is described. The papayas were shipped from Hawaii and irradiated in California under a permit by the Animal and Plant Health Inspection Service and approved by the California Department of Food and Agriculture. Results show that the superior appearance of the irradiated fruit appealed to consumers and that two-thirds or more of the people queried indicated that they would buy irradiated produce. It is noted that this marketing took place in a supportive environment with no protestors present. Informational material was available

  5. Dengue fever treatment with Carica papaya leaves extracts

    OpenAIRE

    Ahmad, Nisar; Fazal, Hina; Ayaz, Muhammad; Abbasi, Bilal Haider; Mohammad, Ijaz; Fazal, Lubna

    2011-01-01

    The main objective of the current study is to investigate the potential of Carica papaya leaves extracts against Dengue fever in 45 year old patient bitten by carrier mosquitoes. For the treatment of Dengue fever the extract was prepared in water. 25 mL of aqueous extract of C. papaya leaves was administered to patient infected with Dengue fever twice daily i.e. morning and evening for five consecutive days. Before the extract administration the blood samples from patient were analyzed. Plate...

  6. Empleo de un Recubrimiento Formulado con Propóleos para el Manejo Poscosecha de Frutos de Papaya (Carica papaya L. cv. Hawaiana) / Use of a Coating Formulated with Propolis for Postharvest Handling of Papaya (Carica papaya L. cv. Hawaiian) Fruits

    Scientific Electronic Library Online (English)

    Elizabeth, Barrera Bello; Marcela, Gil Loaiza; Carlos Mario, García Pajón; Diego Luis, Durango Restrepo; Jesús Humberto, Gil González.

    2012-06-01

    Full Text Available Resumen. El fruto de papaya es reconocido por las propiedades nutricionales y sensoriales; no obstante, su tiempo de vida útil en poscosecha es muy corto. En los últimos años, para incrementar la vida de almacenamiento del fruto se ha implementado el uso de recubrimientos como vehículos de agentes a [...] ntimicrobianos y antioxidantes, entre otros. En este trabajo se comparó el efecto de dos recubrimientos; cera comercial (control) y cera comercial conteniendo un extracto etanólico de propóleos (5% p/v), sobre la vida en poscosecha de frutos de papaya (Carica papaya L. cv. Hawaiana) almacenados a temperatura ambiente (28 ± 2 ºC) y humedad relativa entre 65 y 70%. El efecto de los recubrimientos se determinó mediante el índice de deterioro de los frutos y el recuento microbiano (mesófilos aerobios, mohos y levaduras). Adicionalmente, se evaluaron durante 12 días las propiedades fisicoquímicas de los frutos (cambio de color, textura, pH, acidez total titulable, pérdida de peso y sólidos solubles). Los resultados mostraron que las papayas tratadas con el recubrimiento formulado con el extracto de propóleos, presentó un menor deterioro en cuanto a su apariencia y mayor inhibición del crecimiento de microorganismos durante los primeros 6 días de evaluación en comparación con los frutos control; además, no se observaron diferencias, producto de los recubrimientos, en relación a las características fisicoquímicas de los frutos. Abstract in english Abstract. Papaya fruit is known for the nutritional and sensory properties; however, its postharvest shelf life is very short. In recent years, to extend the storage life of this fruit, the use of coatings as a carrier of antimicrobial agents and antioxidants, has been implemented. In this work, the [...] effect of two coatings, commercial wax (control) and commercial wax containing an ethanolic extract of propolis (5% w/v) on the postharvest of papaya (Carica papaya L. cv. Hawaiian) was compared. The fruits were stored at room temperature (28 ± 2 ºC) and a relative humidity between 65 and 70%. The effect of coatings was determined by the deterioration index of the fruit, and the microbial counts (aerobic mesophiles, molds and yeasts). Additionally, during 12 days were evaluated physicochemical properties of the fruits (color, texture, pH, titratable acidity, weight loss and soluble solids). The results showed that the papaya fruits treated with the coating containing the propolis extract presented less deterioration in their appearance and greater inhibition growth of microorganisms during the first 6 days compared with control fruits; moreover, no differences among the coatings in relation to the physicochemical characteristics of the fruit were observed.

  7. Caracterización y evaluación de dos híbridos de papaya en Cuba / Characterization and evaluation of two papaya hybrids in Cuba

    Scientific Electronic Library Online (English)

    Maruchi, Alonso Esquivel; Yoel, Tornet Quintana; Roberto, Ramos Ramírez; Emilio, Farrés Armenteros; Maikel, Aranguren González; Douglas, Rodríguez Martínez.

    2008-09-01

    Full Text Available La papaya (Carica papaya L.) es considerada como una de las frutas de mayor valor nutritivo y digestivo, siendo utilizada ampliamente en dietas alimenticias, así como gran aceptación a nivel nacional e internacional. Su cultivo puede constituir una gran alternativa para la diversificación agrícola e [...] n las regiones de Cuba, debido a la existencia de áreas con condiciones edafoclimáticas favorables para desarrollar este frutal. Actualmente, los problemas que afectan al cultivo de la papaya son el bajo número de variedades explotadas comercialmente y la susceptibilidad a plagas y enfermedades. Una alternativa viable para la solución de este problema es recurrir a la ampliación de la base genética del papayo mediante la obtención de híbridos con resistencia a plagas y enfermedades, lo que contribuirá de manera decisiva en el mejoramiento del cultivo. El objetivo de esta investigación fue realizar la caracterización fenológica y productiva de dos híbridos de papaya "HGxMA" y "HGxMR" perteneciente al banco de germoplasma de este frutal ubicado en la Unidad Científico-Tecnológica de Base (UCTB) Jagüey Grande, Matanzas del período enero 2005 a noviembre 2007. Los resultados corroboraron que los híbridos presentan características fenotípicas del grupo formosa, frutos con forma elongata en las plantas hermafroditas. El peso medio de los frutos fue de 1.80 kg ("HGxMR") y 2.7 kg ("HGxMA"), de pulpa color naranja-rojiza y amarilla, respectivamente, así como, con una productividad entre 63.4 y 99.8.5 kg planta-1, características que evidencian la posibilidad de utilizarlos en programas de mejoramiento genético del cultivo y en el uso directo por los productores. Actualmente, el mercado consumidor de frutas de papaya de gran tamaño a nivel mundial va creciendo de manera considerable. Por estas razones, estos cultivares de papaya pueden constituir una opción con mayores potencialidades para satisfacer la demanda de los consumidores del cultivo. Abstract in english Papaya is considered one of the fruits of greatest nutritional and digestive value. It is widely used in diets and it has a high acceptance at national and international level. Its culture can constitute a great alternative for agricultural diversification in Cuba regions, due to the existence of ar [...] eas with favorable edaphoclimatic conditions to develop this fruit tree. Nowadays the problems that affect papaya culture are the low number of commercially developed varieties and the susceptibility to pests and diseases. A viable alternative to solve this problem is to widen the genetic papaya base obtaining hybrids with resistance to pest and disease that will contribute decisively to the culture improvement. In this work, the phenology and productive characterization has been done of two papaya hybrids "HGxMA" and "HGxMR" belonging to the germoplasm bank of this fruit tree, located at the Unidad Cientifico-Tecnologica de Base (UCTB) Jagüey Grande, Matanzas. The results confirm that the hybrids present phenotypical characteristics of the Formosa Group, with elongated fruits in hermaphroditic plants. The fruit average weigtht is 1.80 kg ("HGxMR") and 2.7 kg ("HGxMA"), of range-reddish and yellow pulp, respectively and productivity between 63.4 and 99.8.5 kg planta-1, characteristic that show the possibility of use them in genetic improvement programs and the direct use by producers. At present, the markert of big papaya fruits is increasing considerably worldwide. For these reasons, these papaya cultivars can constitute an option with greater potentialities to satisfy consumers demand of the culture.

  8. A coating of chitosan and propolis extract for the postharvest treatment of papaya (Carica papaya L. cv. Hawaiiana

    Directory of Open Access Journals (Sweden)

    Elizabeth Barrera

    2015-06-01

    Full Text Available Propolis is a natural antimicrobial that can be used asa bioadditive in coatings to control fruit quality losses. The effectof two coatings was evaluated, a control (chitosan, 1% and atreatment (chitosan, 1%; containing propolisethanolic extract, 5%,on the microbiological and physicochemical properties of papayafruits. The chemical profile of the propolis revealed the presenceof fatty acids and their esters, carbohydrates, diterpenic acids,and pentacyclic triterpenes. The fruits covered with the treatmentdemonstrated a reduced deterioration index and infection diameterof the fungus Colletotrichum gloeosporioides, as compared tothe control papayas, postponing the appearance of damage bytwo days. Additionally, the treatment did not significantly affectthe physicochemical properties of the papaya, as compared tothe control. In conclusion, the coating formulated with propolisexhibited an in situ fungicidal and bactericidal effect withoutaltering the physiological changes of the papaya fruit duringstorage.

  9. Effect of maleic hydrazide and waxing on quality and shelf life of papaya (carica papaya L.) fruits

    International Nuclear Information System (INIS)

    The effect of post harvest treatment of maleic hydrazide (MH) with and with out waxing on the quality and shelf-life of Baladi and Ekostika I papaya fruits at 18 ±1°C and 85%-90% relative humidity was evaluated. Maleic hydrazide at 250 and 500 ppm significantly delayed fruit ripening by two and three days in both papaya cultivars, respectively, compared with untreated fruits. The higher the concentration, the more was the delay in fruit ripening. The results also showed that waxing addition to MH resulted in a delay of two more days in fruit ripening that treatment with MH alone. The effect of MH and waxing treatments in delaying papaya fruits ripening was manifested in retarded respiratory climacteric, reduced weight loss and delayed fruit softening and increase in total soluble solids and ascorbic acid content.(Author)

  10. Estimation of papaya leaf area using the central vein length

    Directory of Open Access Journals (Sweden)

    Campostrini Eliemar

    2001-01-01

    Full Text Available Four genotypes of papaya (Carica papaya L. two from the 'Solo' group (Sunrise Solo and Improved Sunrise Solo line 72/12 and two from the 'Formosa' group (Tainung 02 and Known-You 01, grown in Macaé, RJ, Brazil (lat. 22(0 24' S, long. 41(0 42' W, were used in this study. Twenty-five mature leaves from each genotype were sampled four and five months after seedling transplant to the field to determine the length of the leaf central vein (LLCV and the leaf area (LA. According to covariance analyses there were no significant differences in the slope and intercept of the mathematical models calculated for each genotype. Thus, a single mathematical model (Log LA = 0.315 + 1.85 Log LLCV, R²=0.898 was adjusted to estimate the LA using the length of LLCV for the four genotypes. An unique model can be applied to estimate the LA for the four papaya genotypes using LLCV in the range from 0.25 to 0.60 m, and for papaya trees 150 to 180 days after transplanting.

  11. 75 FR 22207 - Importation of Papayas From Colombia and Ecuador

    Science.gov (United States)

    2010-04-28

    ... published in the Federal Register (74 FR 18161-18166, Docket No. APHIS-2008-0050) a proposal\\1\\ to amend the... Colombia and Ecuador AGENCY: Animal and Plant Health Inspection Service, USDA. ACTION: Final rule. SUMMARY... importation of commercial shipments of fresh papayas from Colombia and Ecuador into the continental...

  12. 75 FR 22207 - Importation of Papayas From Colombia and Ecuador

    Science.gov (United States)

    2010-04-28

    ...less than half ripe, or ``green,'' are poor hosts for the...allowing the exportation of only green papayas, and trapping for fruit...of Subjects in 7 CFR Part 319 Coffee, Cotton, Fruits, Imports...yellow, surrounded by light green), and appeared to be...

  13. Papaya fruit quality management during the postharvest supply chain

    Science.gov (United States)

    Papayas are popular in tropical and subtropical regions and are being exported in large volumes to Europe, the U.S. and Japan. The fruit has excellent taste, exotic flavor and nutritional properties, being rich in vitamins A, C, and antioxidants. However, due to its highly perishable nature it has n...

  14. Morfología de la flor y de la semilla de papaya (Carica papaya L.): variedad Maradol e híbrido Tainung-1 / Floral and seed morphology of papaya (Carica papaya L.): Maradol variety and Tainung-1 hybrid

    Scientific Electronic Library Online (English)

    Arlette Ivonne, Gil; Diego, Miranda.

    2005-07-01

    Full Text Available Con el objetivo de analizar la morfología de la flor y de la semilla de papaya (Carica papaya L.) de la variedad 'Maradol' y el híbrido 'Tainung-1', se recolectó el material vegetal en dos plantaciones y se llevaron al laboratorio de Fisiología de Cultivos, Facultad de Agronomía, Universidad Naciona [...] l de Colombia, Bogotá, con el fin de realizar las descripciones correspondientes de los tres tipos de flores (femeninas, hermafroditas y estaminadas) y de la semilla, sus características externas (forma, tamaño, hilo, micrópilo, funículo, rafe y abierta seminal) e internas (cubierta seminal, endospermo y embrión). Se concluyó principalmente que a nivel morfológico las flores y semillas de los dos materiales evaluados mostraron características similares, por tanto, las descripciones dadas en este trabajo son generales para ambos. Abstract in english Vegetal material from papaya (Carica papaya L.) 'Maradol' variety and 'Tainung-1' hybrid were collected from two plantations and taken to the Plant Physiology laboratory to analyse their flower and seed morphology. Descriptions were made of the three types of flowers (female, hermaphrodite and stami [...] nate) and the seeds' external (shape, size, hilum, micropyle, funicle, raphe and testa) and internal characteristics (testa, endosperm and embryo). The main morphological conclusion was that the flowers and seeds from the two types evaluated showed similar characteristics; the descriptions given in this paper are thus general for them.

  15. Callus induction in papaya (Carica papaya L. and synseed production for low temperature storage and cryopreservation

    Directory of Open Access Journals (Sweden)

    Teixeira da Silva Jaime A.

    2014-12-01

    Full Text Available The mid- to long-term preservation of papaya (Carica papaya L. would allow for the safeguarding of important germplasm. In this study, soft friable callus (SFC and hard callus (HC were induced from the first two true leaves of 10-day-old seedlings containing a midrib derived from the germinated seed of two cultivars (‘Rainbow’ and ‘Sunrise Solo’. Following germination on a Murashige and Skoog (MS medium that contained 3% sucrose and was free of plant growth regulators (PGRs, sections of the first true leaves from 10-day-old seedlings were exposed to seven published callus or somatic embryogenesis protocols for zygotic embryos, leaves or hypocotyls. Optimal SFC and HC induction was carried out on a half-strength MS medium following the Fitch (1993 or the Ascêncio-Cabral et al. (2008 protocol, respectively. SFC formed shoots that could then convert to plants when transferred to a full-strength MS medium devoid of PGRs. Plantlets 10-cm tall were acclimatised in two steps: first by in vitro acclimatisation in aerated vessels, the Vitron, under CO2-enriched (3000 ppm CO2, then by the transfer of individually rooted plantlets in Rockwool® blocks to a substrate of soil: pine bark : perlite (1:1:1, v/v/v. SFC and HC were then encapsulated in alginate beads, which were exposed to low temperature storage (LTS at 10°C and 15°C, and also cryopreserved for 30 days. All encapsulated alginate beads that contained SFC, HC or leaf tissue that had been stored under LTS or cryopreserved were able to regenerate callus when placed on an optimal callus induction medium. Plants derived from the control, LTS and cryopreservation protocols, either from SFC or HC, were successfully acclimatised.

  16. Sensitivity of papaya seeds to gamma-irradiation

    International Nuclear Information System (INIS)

    The major problems faced by the papaya industry in Malaysia are the papaya ringspot virus disease and the short shelf-life of the fruit. Efforts have been made to address these problems and they include conventional breeding and selection and biotechnological tools for development of transgenic papayas with PRSV resistance and delayed fruit ripening characteristics. Irradiation-induced changes in papaya for selection of improved varieties could offer another approach to resolve these problems. As the first step, the radio-sensitivity of papaya seed must be established before mass irradiation of the materials to generate the desired variability for evaluation and selection later. For the first approximation dosage, the seeds of two local. varieties, Sekaki and Eksotika were irradiated as either dry seeds or as pre-germinated seeds (soaked overnight in water and surface-dried). The dosage ranged from 0 to 300 Gy. Wet pre-germinated seeds were all killed at 100 Gy while dry seeds did not show loss of viability even at 300 Gy. The dosage for dry seeds was raised to 1000 Gy in the following experiment and the LD 50 (or half-kill) was estimated to be 650 Gy. However, at this dosage, seedling growth was markedly reduced and this dosage may not be suitable for mass irradiation. From the growth data, it was estimated that 525 Gy was the dosage that reduced 50% of growth in height and this dosage was recommended for mass irradiation of dry seeds. For wet, pre-germinated seeds, results from the third experiment indicated that 42.5 Gy was the optimal dosage for mass irradiation. At this dosage, both seed germination and seedling growth was reduced by 50%. (Author)

  17. Anatomia foliar de plantas transgênicas e não transgênicas de Carica papaya L. (Caricaceae) / Leaf anatomy of genetically modified and wild-type Carica papaya L. (Caricaceae)

    Scientific Electronic Library Online (English)

    Marcos Vinicius, Leal-Costa; Márcia, Munhoz; Paulo Ernesto, Meissner Filho; Fernanda, Reinert; Eliana Schwartz, Tavares.

    2010-06-01

    Full Text Available O mamoeiro, Carica papaya L. (Caricaceae) é uma espécie americana, cujos frutos são largamente consumidos em todo mundo. Devido às perdas de produção provocadas por viroses e a dificuldade em controlá-las por métodos convencionais, a espécie tem sido alvo de pesquisas de melhoramento genético envolv [...] endo transgenia para resistência a vírus. O presente trabalho descreve a anatomia foliar de plantas de mamoeiro convencional e transgênico resistente ao vírus da mancha anelar-Papaya ringspot virus (PRSV) com inserção da capa protéica viral. As duas cultivares apresentam pecíolo com endoderme e fibras pericíclicas. As folhas são hipoestomáticas e dorsiventrais, com laticíferos acompanhando os feixes vasculares e grande concentração de idioblastos com drusas de oxalato de cálcio. A epiderme é glabra, possuindo estômatos anomocíticos e anisocíticos, com células de paredes anticlinais retas na face adaxial e levemente sinuosas na face abaxial. O presente trabalho concluiu que o processo de transformação genética não alterou as características anatômicas das folhas de C. papaya, servindo de subsídio para avaliação da conformidade anatômica da cultivar transgênica. Abstract in english Papaya, Carica papaya L. (Caricaceae), is an American species, consumed worldwide. A major limitation to papaya production is attack by viruses, like the papaya ringspot virus (PRSV). Papaya has been genetically modified to increase its resistance to PRSV. The aim of this research was to compare the [...] leaf anatomy of wild-type and genetically modified (GM) C. papaya plants to evaluate the influence of genetic modification on leaf anatomy. Wild-type and GM plants showed petiole with endodermis and pericycle fibers. The leaves are hypostomatic and dorsiventral, with laticifers along vascular system and abundant druses of calcium oxalate. The epidermis was glabrous and presented anomocytic and anisocytic stomata, straight anticlinal walls on the adaxial face and sinuous on the abaxial face. Anatomical differences between wild-type and GM C. papaya leaves were not observed. These data contribute to risk assessments regarding the anatomical conformity of GM plants.

  18. In vitro erythrocyte membrane stabilization properties of Carica papaya L. leaf extracts

    OpenAIRE

    Ranasinghe, Priyanga; Ranasinghe, Pathmasiri; W P Kaushalya M. Abeysekera; Premakumara, G. A. Sirimal; Perera, Yashasvi S; Gurugama, Padmalal; Gunatilake, Saman B

    2012-01-01

    Background: Carica papaya L. fruit juice and leaf extracts are known to have many beneficial medical properties. Recent reports have claimed possible beneficial effects of C. papaya L. leaf juice in treating patients with dengue viral infections. This study aims to evaluate the membrane stabilization potential of C. papaya L. leaf extracts using an in vitro hemolytic assay. Materials and Methods: The study was conducted in between June and August 2010. Two milliliters of blood from healthy vo...

  19. In vitro erythrocyte membrane stabilization properties of Carica papaya L. leaf extracts

    OpenAIRE

    Priyanga Ranasinghe; Pathmasiri Ranasinghe; W P Kaushalya M. Abeysekera; G A Sirimal Premakumara; Perera, Yashasvi S; Padmalal Gurugama; Gunatilake, Saman B

    2012-01-01

    Background : Carica papaya L. fruit juice and leaf extracts are known to have many beneficial medical properties. Recent reports have claimed possible beneficial effects of C. papaya L. leaf juice in treating patients with dengue viral infections. This study aims to evaluate the membrane stabilization potential of C. papaya L. leaf extracts using an in vitro hemolytic assay. Materials and Methods: The study was conducted in between June and August 2010. Two milliliters of blood from healthy v...

  20. Evaluation of platelet augmentation activity of Carica papaya leaf aqueous extract in rats

    OpenAIRE

    Swati Patil; Supritha Shetty; Rama Bhide; Shridhar Narayanan

    2013-01-01

    Carica papaya leaves have been used traditionally to treat indigestion, as a vermifuge. Carica papayaleaves have also been shown to possess anti-tumor and immunomodulatory effects. The current study aims at determining the effect of Carica papaya leaves aqueous extract in increasing the platelet count in thrombocytopenic rat model. Aqueous extract of Carica papaya leaves at concentration of 400mg/kg and 800mg/kg were given to cyclophosphamide induced thrombocytopenic rats for a period of fift...

  1. Nematodos agalladores asociados al cultivo de papaya (Carica papaya L.) en el departamento de Córdoba, Colombia / Root knot nematodes associated with papaya crop (Carica papaya L.) in department of Córdoba, Colombia

    Scientific Electronic Library Online (English)

    Juan de Dios, Jaraba; Zaida, Lozano; Manuel, Espinosa.

    2007-06-01

    Full Text Available El objetivo de este trabajo fue identificar los nematodos agalladores asociados al cultivo de papaya (Carica papaya L.) en los municipios de Tierralta, Valencia, Lorica, Montería y Montelíbano que constituyen el área productora de papaya en Córdoba. Se evaluaron variables morfológicas y morfométrica [...] s en hembras, machos y juveniles de segundo estadío (J2). En las muestras de suelo se determinó pH, contenido de materia orgánica (MO), conductividad eléctrica (CE) y textura, a fin de correlacionarlas con la presencia de especies nematodas. Se encontraron Meloidogyne javanica, M. incognita y M. arenaria, siendo M. incognita la especie más frecuente. Las tres especies se encontraron mezcladas en 37,5% de los individuos; M. incognita y M. arenaria en 50%, mientras que M. incognita y M. javanica no se encontraron mezcladas. Las especies se encontraron en pH de 4,9 a 6,4; CE de 0,1 a 0,45 dS· m-1; MO de 1,2 % a 2,5 % y la textura fue arenosa, franca y franco arenosa. El pH, el contenido de arena y la CE correlacionan de manera positiva con la presencia de los nematodos, mientras a MO mostró correlación negativa. Se reporta por primera vez la ocurrencia de M. javanica, M. incognita, M. arenaria, así como la mezcla de M. incognita y M. arenaria, en papayas de Colombia. Abstract in english Nematode species associated with papaya crop (Carica papaya L) were characterized in Cordoba, Colombia by using morphological and morphometric parameters of their females, males, and infective second-stage juveniles (J2). Soil samples were taken from papaya plantations in order to evaluate soil text [...] ure, organic matter (OM), pH, electric conductivity (EC), and, finally, presence of root knot nematodes in the soil. Correlation analysis was performed to correlate soil variables with the presence of Meloidogyne species. Meloidogyne incognita, M. arenaria and M. javanica were found, whereas M. incognita was the most frequent specie. These three species were found together in 37.5% of the soils of papaya plantation; M. incognita and M. arenaria were mixed in 50%, while M. javanica and M. incognita were not mixed. Conducive soils to Meloidogyne species had pH 4.9 to 6.4, EC 0.1 to 0.45 dS· m-1, OM 1.2 to 2.5 %, and sandy to sandy-loam soil texture. The pH, percentages sand and CE were positively correlated with an abundance of Meloidogyne species, whereas OM was negatively correlated. This is the first report of M. javanica, M. incognita and M. arenaria, and the mixture of M. incognita and M. arenaria in papaya plantations of Colombia.

  2. THERAPEUTIC POTENTIAL OF CARICA PAPAYA L. LEAF EXTRACT IN TREATMENT OF DENGUE PATIENTS

    Directory of Open Access Journals (Sweden)

    CVM Naresh Kumar

    2015-07-01

    Full Text Available Carica papaya leaf extracts are known to have many beneficial medicinal properties. Recent studies have claimed possible beneficial effects of Carica papaya leaf extracts in treating patients with dengue infection. The present study aims to evaluate the therapeutic potential of Carica papaya leaf extracts in treating dengue patients. Carica papaya leaf extracts were given to dengue infected patients and their blood samples were daily monitored. Polyserositis condition of the patients was analysed by ultrasonography before and after the completion of Carica papaya leaf extract treatment. Repeated Measures ANOVA and descriptive measures such as mean, standard error values were calculated. Before the administration of Carica papaya leaf extracts the patients had a low WBC and platelet counts. After the administration of Carica papaya leaf extracts there was a good amount of increase in the counts of WBC and platelets (p<0.05 in all the patients and the patients started recovering from dengue infection. Ultrasonographic findings revealed clearance in pleural ascites, pericardiac effusion and peritoneal ascites. Blood analysis reports, ultrasonographic findings, statistical results and patients recovery from dengue infection clearly shows the therapeutic role of Carica papaya leaf extracts in treating dengue infected patients. Further studies are necessary for identification of the compounds present in Carica papaya leaf extract and exploring their therapeutic role in curing dengue infection.

  3. Situación actual y perspectivas tecnológicas para la papaya (Carica papaya L.) en el distrito de Veracruz, Veracruz / Current situation and technological prospects for papaya (Carica papaya L.) in the district of Veracruz, Veracruz

    Scientific Electronic Library Online (English)

    Rebeca, Granados Ramírez; Rafael, Salceda Lopez; María del Pilar, Longar Blanco.

    2015-06-01

    Full Text Available La papaya es la tercera fruta tropical más consumida en el mundo y por su dinamismo comercial es considerada una de las más importantes desde el punto de vista económico. México ocupa el sexto lugar como productor mundial con 712 917 t anuales, de las cuales una quinta parte se destina al mercado ex [...] terior. El objetivo de este trabajo fue analizar las innovaciones tecnológicas utilizadas durante el proceso de producción de la Carica papaya L. en el DDR 007 Veracruz, e identificar las etapas vulnerables y sugerir estrategias. La metodología incluyó la evaluación del coeficiente de localización, análisis del proceso de producción con énfasis en la utilización de tecnologías de vanguardia, mediante la obtención de información en campo en las comunidades productoras. Se integró la matriz FODA donde se identificaron las fortalezas y factores críticos, oportunidades y amenazas de la actividad frutícola. Se destacaron las condiciones edafo-climáticas para la producción, elevada rentabilidad y rápido retorno de la inversión (fortalezas). De lo anterior, se puede inferir que asignar mayores superficies de tierra al cultivo de papaya en la zona, con certeza será una opción viable para la adquisición de mayores ingresos económicos para los productores locales; además de un creciente consumo y las grandes posibilidades de exportación (oportunidades). Se subraya la necesidad de adicionar biotecnología para retrasar el proceso de maduración, además obtener versatilidad del fruto para su consumo. Abstract in english Papaya is the third most consumed tropical fruit in the world and its commercial dynamism is considered one of the most important from the economic point of view. Mexico ranks sixth as a world producer with 712 917 t, of which one-fifth goes to foreign markets annually. The aim of this study was to [...] analyse the technological innovations used during production of Carica papaya L. in the DDR 007 Veracruz, and identify vulnerable stages and suggest strategies. The methodology included assessment of the location coefficient, analysis of the production process with emphasis on the use of advanced technologies, by obtaining information field producing communities. A SWOT matrix was integrated where the strengths and critical factors, opportunities and threats identified fruit activity. The leaf-climatic conditions were highlighted for the production of high profitability and rapid return on investment (strengths). From the foregoing is inferred that allocating more land areas with papaya crop in the area, for sure will be an option to acquire higher incomes for local producers; besides increasing consumption and large export possibilities (opportunities). The need to add biotechnology to delay the ripening process is emphasized and also getting versatile fruits for consumption.

  4. Cyanogenesis in glucosinolate-producing plants: Carica papaya and Carica quercifolia

    DEFF Research Database (Denmark)

    Olafsdottir, E.S.; JØrgensen, Lise Bolt

    2002-01-01

    Carica papaya, Carica quercifolia, Carica hastata, Caricaceae, Passifloraceae, Biosynthesis, Glucosinolates, Cyanohydrin glycosides, Cyanogenic glycosides, Prunasin, Tetraphyllin B, Cyclopentenylglycine

  5. Dormancia en semillas de papaya cv maradol roja durante el almacenamiento / Dormancy of papaya seeds cv Maradol Roja during storage

    Scientific Electronic Library Online (English)

    Maruchi, Alonso-Esquivel; Yoleinis, Ortiz-López; Roberto, Ramos-Ramírez; Hugo, Oliva-Diaz; Maricela, Capote-del Sol.

    2011-12-01

    Full Text Available Dormancia en semillas de papaya cv Maradol Roja durante el almacenamiento. El objetivo del presente trabajo fue determinar el efecto de la humedad de la semilla cultivar Maradol Roja y la temperatura durante el almacenamiento, en la dormancia de la papaya. Este trabajo fue desarrollado en el Laborat [...] orio Nacional de Semillas de la Empresa Productora y Comercializadora de Semillas, provincia La Habana, Cuba, en el período noviembre 2006 a enero 2008. Fueron extraídas las semillas de frutos de papaya cv Maradol Roja, y se secaron a tres contenidos de humedad (12%, 10,56% y 9,26%), y acondicionadas en bolsas de polietileno y almacenadas a 15ºC y 4ºC. El porcentaje de germinación, altura y peso fresco de la planta fueron realizadas al inicio y después de tres, seis, nueve y doce meses de almacenamiento. Hubo tendencia a la disminución de la germinación en los primeros seis meses del almacenamiento, alcanzándose los valores más bajos del porcentaje de germinación (55,6%). A partir de los nueve y doce meses fue detectado un aumento significativo en la germinación, independiente del contenido de humedad de la semilla. El ambiente de 15ºC favoreció mayor germinación en períodos mayores de almacenamiento en relación a las mantenidas a 4ºC, que exhibieron mejores porcentajes de germinación en un período menor (tres meses de almacenamiento). La dormancia finalizó en los periodos de almacenamiento a los nueve y doce meses, independientemente del contenido de humedad. Abstract in english Dormancy of papaya seeds cv Maradol Roja during storage. The objective of this work was to determine the effect of humidity content of papaya cv Maradol Roja and storage temperatura on dormany. The work was conducted at the Laboratorio Nacional de Semillas, Empresa Productora y Comercializadora de S [...] emillas, La Habana, Cuba, from November 2006 to January 2008. Seeds were extracted from papaya fruits cv Maradol Roja and dried to three humidiy contents (12%, 10,56% y 9,26%), and aconditioned in polyiethylene bags and stored at 15ºC and 4ºC. Percentaje of seed germination, heigth and fresh weight of the seedlings were measured at 3, 6, 9 and 12 months in storage. There was a trend towards reduced seed germination during the first 6 months in storage (55,6%). After 9 and 12 months a significant incresase in germination was observed, independent of seed water content. Seeds stored in environment at 15ºC favored germination after longer sotrage compared to seeds kept in storage for shorter periods (3 months). Seed dormany concluded between 9 and 12 months in storage irrespective of seed water content.

  6. 40 CFR 174.515 - Coat Protein of Papaya Ringspot Virus; exemption from the requirement of a tolerance.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Coat Protein of Papaya Ringspot Virus...-INCORPORATED PROTECTANTS Tolerances and Tolerance Exemptions § 174.515 Coat Protein of Papaya Ringspot Virus; exemption from the requirement of a tolerance. Residues of Coat Protein of Papaya Ringspot Virus are...

  7. Anatomy of somatic embryogenesis in Carica papaya L.

    OpenAIRE

    Juliana A. Fernando; Murilo de Melo; Marli K. M. Soares; Beatriz Appezzato-da-Glória

    2001-01-01

    Mature zygotic embryos of Carica papaya L. ‘Sunrise Solo’ were used as explants for embryogenesis induction. The explants were inoculated on Murashige and Skoog culture medium supplemented with 2 mg.L-1 2,4-dichlorophenoxyacetic acid and incubated in darkness at 25+2°C. Histological analysis of callogenesis and somatic embryogenesis indicated occurrence of direct and indirect somatic embryogenesis development. Direct somatic embryo formation was observed from hypocotyledonary epidermic cells ...

  8. INFLUENCE OF THICKNESS ON THE DRYING OF PAPAYA PUREE (Carica papaya L.) THROUGH REFRACTANCE WINDOWTM TECHNOLOGY / INFLUENCIA DEL ESPESOR EN SECADO DE PURÉ DE PAPAYA (Carica Papaya L.) POR TECNOLOGÍA DE VENTANA DE REFRACTANCIA®

    Scientific Electronic Library Online (English)

    MARÍA U., OCORÓ-ZAMORA; ALFREDO A., AYALA-APONTE.

    2013-12-01

    Full Text Available El objetivo de este trabajo fue evaluar la influencia del espesor de las muestras (2, 3 y 4 mm) sobre las cinéticas de secado, actividad de agua (a w) y color (deltaE) de rodajas de puré de papaya procesadas por tecnología de ventana de refractanciaTM (RW™). Adicionalmente se evaluó la difusividad d [...] el agua (Deff) teniendo en cuenta el encogimiento. Los datos de humedad se ajustaron mediante los modelos de Newton y Midilli. Los resultados mostraron que a menor espesor el secado fue más rápido, los valores de a w fueron menores y los deltaE superiores. Las muestras alcanzaron 0.0652, 0.1132 y 0.2624 g agua/ g sólido seco en 60 min para rodajas de 2, 3 y 4 mm, respectivamente. El Modelo de Midilli fue el más apropiado para predecir las curvas de secado de papaya por RW™. Deff disminuyó a menor espesor y su orden de magnitud fue de 10-10 m²/s. Abstract in english The aim of this study was to evaluate the influence of sample thickness (2, 3 and 4 mm) on the drying kinetics, water activity (a w) and color (deltaE) of papaya puree slices processed through Refractance WindowTM technology (RW™). Additionally, the water diffusion coefficient (Deff) was evaluated t [...] aking into account the shrinkage effect. The experimental values of moisture were fitted using Newton's and Midilli's models. The results showed that the lower the thickness, the faster the drying, the lower the values of a w and the higher the deltaE. The samples reached 0.0652, 0.1132 and 0.2624 g water/ g dry solid in 60 min for 2, 3 and 4 mm slices, respectively. Midilli's model was the most appropriate to predict the experimental curves of papaya drying through RW™. Deff decreased at a lower thickness and its order of magnitude was of 10-10 m²/s.

  9. Características fenotípicas y agronómicas de seis genotipos de papaya (Carica papaya L. de Tuxpan, Guerrero, México

    Directory of Open Access Journals (Sweden)

    José Ángel Alcántara Jiménez

    2010-07-01

    Full Text Available En México la producción de papayo (Carica papaya L. es afectada por factores genéticos, ambientales y bióticos. Destacan los daños provocados por el virus de la mancha anular, el cual reduce considerablemente la cantidad y calidad de la fruta; por eso es muy importante, conocer la productividad de los genotipos en un ambiente determinado. La presente investigación se realizó con el objetivo de evaluar el desarrollo y las características agronómicas de seis genotipos de papayo. El estudio se llevó a cabo en la localidad de Tuxpan, Guerrero, donde se cultivaron los genotipos: R4M3, Maradol, Criolla, Red Lady, Zapote y R5M2. Como diseño experimental se utilizó el de bloques completos al azar, con cuatro repeticiones. Las variables evaluadas fueron: altura al primer fruto, diámetro del cuello, altura de la planta, número de días a la cosecha, rendimiento de frutos (t/ha, número, diámetro y longitud de frutos, concentración de azúcares (grados Brix, textura, color de la pulpa y epicarpio de frutos maduros. Los datos de las variables se sometieron a análisis de varianza y prueba de comparación de medias de Tukey. En relación a la adaptación de los genotipos: todos los genotipos se desarrollaron y produjeron frutos en las condiciones de trópico seco. La variedad Maradol fue la más sobresaliente, presentando un porte menor, mayor precocidad y productividad, textura dura de pulpa, alta concentración de azúcares, buen tamaño y color.

  10. Ripening behavior of papaya (Carica papaya L.) exposed to gamma irradiation

    International Nuclear Information System (INIS)

    Papaya fruit treated with 250 Gy of ?-irradiation frequently softened more uniformly than non-irradiated fruit. Fruit with less than 25% of their surface colored yellow placed immediately into storage at 10 °C after irradiation developed skin scald. This was prevented by delaying storage by 12 h. Fruit that were irradiated when 30% of the skin was yellowed softened at a slower rate than non-irradiated fruit. There was no difference in softening rate between irradiated and non-irradiated fruit at the mature green stage. Fruit stored for 14 days at 10 °C before returning to 25 °C had a slightly slower rate of softening than fruit allowed to ripen at 25 °C without storage. Premature flesh softening occurred occasionally in fruit that had between 8 and 18% of the skin yellow and 70–90% flesh coloring when irradiated. Premature softening occurred in the tests run on fruit that were harvested during the warmer months; fruit harvested during the cooler months did not show the condition. The conditions of growth that predispose fruit to more rapid softening following ?-irradiation were not determined

  11. Características fenotípicas y agronómicas de seis genotipos de papaya (Carica papaya L. de Tuxpan, Guerrero, México

    Directory of Open Access Journals (Sweden)

    José Ángel Alcántara Jiménez

    2010-06-01

    Full Text Available En México la producción de papayo (Carica papaya L. es afectada por factores genéticos, ambientales y bióticos. Destacan los daños provocados por el virus de la mancha anular, el cual reduce considerablemente la cantidad y calidad de la fruta; por eso es muy importante, conocer la productividad de los genotipos en un ambiente determinado. La presente investigación se realizó con el objetivo de evaluar el desarrollo y las características agronómicas de seis genotipos de papayo. El estudio se llevó a cabo en la localidad de Tuxpan, Guerrero, donde se cultivaron los genotipos: R4M3, Maradol, Criolla, Red Lady, Zapote y R5M2. Como diseño experimental se utilizó el de bloques completos al azar, con cuatro repeticiones. Las variables evaluadas fueron: altura al primer fruto, diámetro del cuello, altura de la planta, número de días a la cosecha, rendimiento de frutos (t/ha, número, diámetro y longitud de frutos, concentración de azúcares (grados Brix, textura, color de la pulpa y epicarpio de frutos maduros. Los datos de las variables se sometieron a análisis de varianza y prueba de comparación de medias de Tukey. En relación a la adaptación de los genotipos: todos los genotipos se desarrollaron y produjeron frutos en las condiciones de trópico seco. La variedad Maradol fue la más sobresaliente, presentando un porte menor, mayor precocidad y productividad, textura dura de pulpa, alta concentración de azúcares, buen tamaño y color.

  12. Dengue fever treatment with Carica papaya leaves extracts.

    Science.gov (United States)

    Ahmad, Nisar; Fazal, Hina; Ayaz, Muhammad; Abbasi, Bilal Haider; Mohammad, Ijaz; Fazal, Lubna

    2011-08-01

    The main objective of the current study is to investigate the potential of Carica papaya leaves extracts against Dengue fever in 45 year old patient bitten by carrier mosquitoes. For the treatment of Dengue fever the extract was prepared in water. 25 mL of aqueous extract of C. papaya leaves was administered to patient infected with Dengue fever twice daily i.e. morning and evening for five consecutive days. Before the extract administration the blood samples from patient were analyzed. Platelets count (PLT), White Blood Cells (WBC) and Neutrophils (NEUT) decreased from 176×10(3)/µL, 8.10×10(3)/µL, 84.0% to 55×10(3)/µL, 3.7×10(3)/µL and 46.0%. Subsequently, the blood samples were rechecked after the administration of leaves extract. It was observed that the PLT count increased from 55×10(3)/µL to 168×10(3)/µL, WBC from 3.7×10(3)/µL to 7.7×10(3)/µL and NEUT from 46.0% to 78.3%. From the patient feelings and blood reports it showed that Carica papaya leaves aqueous extract exhibited potential activity against Dengue fever. Furthermore, the different parts of this valuable specie can be further used as a strong natural candidate against viral diseases. PMID:23569787

  13. A rapid detection for irradiated fresh papayas using ESR spectroscopy

    International Nuclear Information System (INIS)

    The detections of radicals induced in irradiated both fresh and dried papayas were carried out at liquid nitrogen and room temperature using electron spin resonance (ESR) spectroscopy. Fresh papayas irradiated by the ?-rays were separated into flesh and skin that subjected to the ESR detection. The ESR spectra of the specimen at liquid-nitrogen temperature were observed clearly at a week after the ?-irradiation. Those signals were consisted from main peak at g=2.000 and side peaks at g=2.018 and 1.982. They showed a linear response against the dose. Furthermore, the side peaks from freeze-dried papaya flesh stored at 4degC were observed clearly at two weeks after the ?-irradiation. Those signals also showed the linear dose-response. The detection scheme for irradiated fresh fruits can be done by two stages: 1) A screening test of ESR signals at liquid nitrogen temperature using fresh samples. 2) A room temperature ESR measurement using freeze-dry samples. (author)

  14. Acarofauna associated to papaya orchards in Veracruz, Mexico

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    Marycruz Abato-Zárate

    2014-01-01

    Full Text Available Mexican agriculturists have recently noticed strong increases of mite infestations in papaya (Carica papaya L. 1753 orchards. A list of mite species associated with papaya leaves was constructed to determine the species responsible for high infestations and to identify predaceous mites as potential biological control agents. Mites were collected from three foliage strata (high, middle and low, in seven municipalities of central Veracruz State. Leaves were processed by washing and sieving. Identified species included four tetranychids: Eotetranychus lewisi (McGregor 1943, Eutetranychus banksi (McGregor 1914, Tetranychus merganser Boudreaux 1954, and Tetranychus urticae Koch 1836; as well as three phytoseiids: Euseius hibisci (Chant 1959, Galendromus helveolus (Chant 1959 and Phytoseiulus macropilis (Banks 1905, with the first two species being the most abundant. The vagrant eriophyid Calacarus citrifolii Keifer 1955 was collected in three municipalities, in the low stratum. Neither the broad mite, Polyphagotarsonemus latus (Banks 1904, nor the carmine spider mite, Tetranychus cinnabarinus (Boisduval 1867, were collected, although these species were previously recorded from this area. None of the Phytoseiid species found can be considered a recently established species; their potential as biological control agents is discussed.

  15. Reguladores vegetais na quebra da dominância apical de mamoeiro (Carica papaya L.) / Plant growth regulators on breaking apical dominance in papaya plants (Carica papaya L.)

    Scientific Electronic Library Online (English)

    Elizabeth Orika, Ono; José Francisco, Grana Júnior; João Domingos, Rodrigues.

    2004-08-01

    Full Text Available O trabalho avaliou os efeitos de reguladores vegetais sobre a quebra da dominância apical de mamoeiro (Carica papaya L. cv. Improved Sunrise Solo). A aplicação dos reguladores vegetais foi iniciada quando as plantas tinham seis meses de idade, totalizando três aplicações, a intervalos de sete dias, [...] constando dos seguintes tratamentos: T1- água (testemunha); T2- GA3 250 mg L-1; T3- GA3 500 mg L-1; T4- benziladenina (BA) 250 mg L-1; T5- BA 500 mg L-1; T6- GA3 125 mg L-1 + BA 125 mg L-1; T7- GA3 250 mg L-1 + BA 250 mg L-1. Esses tratamentos foram acompanhados da remoção ou não da gema apical. Os resultados mostraram que plantas tratadas com GA3 + BA a 125 e 250 mg L-1, com e sem a remoção da gema apical, apresentaram maior número de brotações que a testemunha, a qual não apresentou nenhuma brotação das gemas laterais. Abstract in english The objective of this work was to study the effects of gibberelin and cytokinin on breaking apical dominance and axillary buds growth of the Carica papaya L. Papaya plants sprayed three times within a period of 7 days, with the following treatments: T1- water (control), T2- GA3 250 mg L-1, T3- GA3 5 [...] 00 mg L-1, T4- benzyladenine (BA) 250 mg L-1, T5- BA 500 mg L-1, T6- GA3 125 mg L-1 + BA 125 mg L-1, T7- GA3 250 mg L-1 + BA 250 mg L-1. The treatments were carried out with or without removal of the apical buds. After 36 days of the last plant growth regulators application was observed lateral bud numbers, lateral buds length and lateral buds diameter. The results showed that there were a higher number of shoots in plants treated with GA3 at 125 mg L-1 + BA at 125 mg L-1 and GA3 at 250mg L-1 + BA at 250 mg L-1. The removal of the apical bud helped the sprouting of the papaya plants.

  16. Reguladores vegetais na quebra da dominância apical de mamoeiro (Carica papaya L. Plant growth regulators on breaking apical dominance in papaya plants (Carica papaya L.

    Directory of Open Access Journals (Sweden)

    Elizabeth Orika Ono

    2004-08-01

    Full Text Available O trabalho avaliou os efeitos de reguladores vegetais sobre a quebra da dominância apical de mamoeiro (Carica papaya L. cv. Improved Sunrise Solo. A aplicação dos reguladores vegetais foi iniciada quando as plantas tinham seis meses de idade, totalizando três aplicações, a intervalos de sete dias, constando dos seguintes tratamentos: T1- água (testemunha; T2- GA3 250 mg L-1; T3- GA3 500 mg L-1; T4- benziladenina (BA 250 mg L-1; T5- BA 500 mg L-1; T6- GA3 125 mg L-1 + BA 125 mg L-1; T7- GA3 250 mg L-1 + BA 250 mg L-1. Esses tratamentos foram acompanhados da remoção ou não da gema apical. Os resultados mostraram que plantas tratadas com GA3 + BA a 125 e 250 mg L-1, com e sem a remoção da gema apical, apresentaram maior número de brotações que a testemunha, a qual não apresentou nenhuma brotação das gemas laterais.The objective of this work was to study the effects of gibberelin and cytokinin on breaking apical dominance and axillary buds growth of the Carica papaya L. Papaya plants sprayed three times within a period of 7 days, with the following treatments: T1- water (control, T2- GA3 250 mg L-1, T3- GA3 500 mg L-1, T4- benzyladenine (BA 250 mg L-1, T5- BA 500 mg L-1, T6- GA3 125 mg L-1 + BA 125 mg L-1, T7- GA3 250 mg L-1 + BA 250 mg L-1. The treatments were carried out with or without removal of the apical buds. After 36 days of the last plant growth regulators application was observed lateral bud numbers, lateral buds length and lateral buds diameter. The results showed that there were a higher number of shoots in plants treated with GA3 at 125 mg L-1 + BA at 125 mg L-1 and GA3 at 250mg L-1 + BA at 250 mg L-1. The removal of the apical bud helped the sprouting of the papaya plants.

  17. Characterization of a high oleic oil extracted from papaya (Carica papaya L.) seeds / Caracterização de um óleo alto oleico extraído de sementes de mamão (Carica papaya L.)

    Scientific Electronic Library Online (English)

    Cassia Roberta, Malacrida; Mieko, Kimura; Neuza, Jorge.

    2011-12-01

    Full Text Available As características físico-químicas e as composições de ácidos graxos, tocoferóis e carotenoides do óleo bruto extraído de sementes de mamão (Carica papaya L.), variedade formosa, foram investigadas. O rendimento em óleo das sementes foi de 29,16%. Os dados obtidos para os índices analíticos comparam [...] -se bem com os de outros óleos comestíveis. O óleo apresentou elevada resistência à oxidação (77,97 horas). Os principais ácidos graxos quantificados foram o oleico (71,30%), seguido pelo palmítico (16,16%), linoleico (6,06%) e esteárico (4,73%). O ? e ?-tocoferol foram os tocoferóis predominantes com 51,85 e18,9 mg.kg-1, respectivamente. A ?-criptoxantina (4,29 mg.kg-1) e o ?-caroteno (2,76 mg.kg-1) foram os carotenóides quantificados e o conteúdo de compostos fenólicos totais foi de 957,60 mg.kg-1. Assim, a potencial utilização das sementes de mamão para a produção de óleo parece ser favorável. Entretanto, estudos toxicológicos são ainda necessários antes de o óleo ser indicado para utilização em alimentos. Abstract in english The physicochemical characteristics, fatty acid, tocopherol, and carotenoid composition of a crude oil extracted from papaya (Carica papaya L.) seeds, formosa variety, were investigated. The oil yield from the seeds was 29.16%. The data obtained for the analytical indexes were in agreement with thos [...] e of other edible oils. The oil obtained had high oxidation resistance (77.97 hours). The major fatty acids in total lipid were oleic (71.30%), palmitic (16.16%), linoleic (6.06%), and stearic (4.73%) acid. The ? and ?-tocopherol were the predominant tocopherols with 51.85 and 18.89 mg.kg-1, respectivelly. The ?-cryptoxanthin (4.29 mg.kg-1) and ?-carotene (2.76 mg.kg-1) were the carotenoids quantified, and the content of total phenolic compounds was 957.60 mg.kg-1. Therefore, the potential utilization of the papaya seeds for oil production seems favorable. However, toxicological studies need to be carried out before the oil is appropriate for food applications.

  18. Hypoglycemic effect of Carica papaya leaves in streptozotocin-induced diabetic rats

    Directory of Open Access Journals (Sweden)

    Juárez-Rojop Isela Esther

    2012-11-01

    Full Text Available Abstract Background Traditional plant treatment for diabetes has shown a surging interest in the last few decades. Therefore, the purpose of this study was to assess the hypoglycemic effect of the aqueous extract of C. papaya leaves in diabetic rats. Several studies have reported that some parts of the C. papaya plant exert hypoglycemic effects in both animals and humans. Methods Diabetes was induced in rats by intraperitoneal administration of 60 mg/kg of streptozotocin (STZ. The aqueous extract of C. papaya was administered in three different doses (0.75, 1.5 and 3 g/100 mL as drinking water to both diabetic and non-diabetic animals during 4 weeks. Results The aqueous extract of Carica papaya (0.75 g and 1.5 g/100 mL significantly decreased blood glucose levels (pC. papaya could help islet regeneration manifested as preservation of cell size. In the liver of diabetic treated rats, C. papaya prevented hepatocyte disruption, as well as accumulation of glycogen and lipids. Finally, an antioxidant effect of C. papaya extract was also detected in diabetic rats. Conclusions This study showed that the aqueous extract of C. papaya exerted a hypoglycemic and antioxidant effect; it also improved the lipid profile in diabetic rats. In addition, the leaf extract positively affected integrity and function of both liver and pancreas.

  19. Papaya is not a host for Tomato Yellow Leaf Curl Virus

    Science.gov (United States)

    The economic value of tomato production is threatened by tomato yellow leaf-curl virus TYLCV and its vector, the silverleaf whitefly Bemisia tabaci biotype B (Gennadius) (Hemiptera: Aleyrodidae). Use of papaya Carica papaya L. as a banker plant for a whitefly parasitoid shows promise as a whitefly m...

  20. An umbra-like virus of papaya discovered in Ecuador: detection, occurrence and phylogenetic relatedness

    Science.gov (United States)

    Double-stranded RNA (dsRNA) extractions from papaya leaves infected with Papaya ringspot virus (PRSV) revealed the presence of an unusual 4kb band, in addition to the presumed PRSV-associated 10kb band. Partial sequence of RT-PCR products from the 4kb dsRNA revealed homology to genomes of several me...

  1. A Current Overview of the Papaya meleira virus, an Unusual Plant Virus

    Directory of Open Access Journals (Sweden)

    Paolla M. V. Abreu

    2015-04-01

    Full Text Available Papaya meleira virus (PMeV is the causal agent of papaya sticky disease, which is characterized by a spontaneous exudation of fluid and aqueous latex from the papaya fruit and leaves. The latex oxidizes after atmospheric exposure, resulting in a sticky feature on the fruit from which the name of the disease originates. PMeV is an isometric virus particle with a double-stranded RNA (dsRNA genome of approximately 12 Kb. Unusual for a plant virus, PMeV particles are localized on and linked to the polymers present in the latex. The ability of the PMeV to inhabit such a hostile environment demonstrates an intriguing interaction of the virus with the papaya. A hypersensitivity response is triggered against PMeV infection, and there is a reduction in the proteolytic activity of papaya latex during sticky disease. In papaya leaf tissues, stress responsive proteins, mostly calreticulin and proteasome-related proteins, are up regulated and proteins related to metabolism are down-regulated. Additionally, PMeV modifies the transcription of several miRNAs involved in the modulation of genes related to the ubiquitin-proteasome system. Until now, no PMeV resistant papaya genotype has been identified and roguing is the only viral control strategy available. However, a single inoculation of papaya plants with PMeV dsRNA delayed the progress of viral infection.

  2. METHYL JASMONATE REDUCES DECAY AND MAINTAINS POSTHARVEST QUALITY OF PAPAYA 'SUNRISE'

    Science.gov (United States)

    Exposure of papaya (Carica papaya L., cv. Sunrise) fruit to methyl jasmonate (MJ) vapors (ten to the -5 or ten to the -4 M) for 16 h at 20 degrees C inhibited fungal decay and reduced chilling injury development and loss of firmness during storage for 14-32 days at 10 degrees C and shelf life (4 day...

  3. Molecular characterization and pathogenicity of Erwinia spp. associated with pineapple [Ananas comosus (L. Merr.] and papaya (Carica papaya L.

    Directory of Open Access Journals (Sweden)

    Ramachandran Kogeethavani

    2015-12-01

    Full Text Available The Erwinia species are well-known pathogens of economic importance in Malaysia causing serious damage to high-value fruit crops that include pineapple [Ananas comosus (L. Merr.] and papaya (Carica papaya L..The 16S rRNA sequence using eubacteria fD1 and rP2 primers, identified two bacteria species; Dickeya zeae from pineapple heart rot, and Erwinia mallotivora from papaya dieback. Phylogenetic analysis based on the neighbor-joining method indicated that all the bacterial isolates clustered in their own taxa and formed monophyletic clades. From the pathogenicity test, all isolates of D. zeae and E. mallotivora showed pathogenic reactions on their respective host plants. Genetic variability of these isolates was assessed using repetitive sequence-based PCR (rep-PCR fingerprinting. The results indicated interspecies, and intraspecies variation in both species’ isolates. There were more polymorphic bands shown by rep-PCR fingerprints than enterobacterial repetitive intergenic consensus (ERIC and BOX- PCRs, however both species’ isolates produced distinguishable banding patterns. Unweighted pair-group method with arithmetic averages (UPGMA cluster analysis indicated that all Dickeya and Erwinia isolates from the same species were grouped in the same main cluster. Similarity among the isolates ranged from 77 to 99%. Sequencing of 16S rRNA using eubacteria fD1 and rP2 primers, and rep-PCR fingerprinting revealed diversity among Dickeya and Erwinia isolates. But this method appears to be reliable for discriminating isolates from pineapple heart rot and papaya dieback.

  4. Nematodos agalladores asociados al cultivo de papaya (Carica papaya L. en el departamento de Córdoba, Colombia

    Directory of Open Access Journals (Sweden)

    Jaraba Juan de Dios

    2007-06-01

    Full Text Available

    El objetivo de este trabajo fue identificar los nematodos agalladores asociados al cultivo de papaya (Carica papaya L. en los municipios de Tierralta, Valencia, Lorica, Montería y Montelíbano que constituyen el área productora de papaya en Córdoba. Se evaluaron variables morfológicas y morfométricas en hembras, machos y juveniles de segundo estadío (J2. En las muestras de suelo se determinó pH, contenido de materia orgánica (MO, conductividad eléctrica (CE y textura, a fin de correlacionarlas con la presencia de especies nematodas. Se encontraron Meloidogyne javanica,M. incognita y M. arenaria, siendo M. incognita la especie más frecuente. Las tres especies se encontraron mezcladas en 37,5% de los individuos;M. incognita y M. arenaria en 50%, mientras que M. incognita y M. javanica no se encontraron mezcladas. Las especies se encontraron en pH de 4,9 a 6,4; CE de 0,1 a 0,45 dS· m-1; MO de 1,2 % a 2,5 % y la textura fue arenosa, franca y franco arenosa. El pH, el contenido de arena y la CE correlacionan de manera positiva con la presencia de los nematodos, mientras la MO mostró correlación negativa. Se reporta por primera vez la ocurrencia de M. javanica, M. incognita, M. arenaria, así como la mezcla de M. incognita y M. arenaria, en papayas de Colombia.

  5. Hurdle technology to preserve the 'Golden' papaya post harvest quality

    International Nuclear Information System (INIS)

    With the purpose of evaluating a combination of post harvest techniques on papaya Golden storage, the following treatments were investigated: carton boxes packaging (CP); CP + plastic bag of PEBD - low density polyethylene film, with 0.05mm of thickness (PE); CP + PEBD with 0.025mm of thickness impregnated with mineral ethylene scavenger (PEAbs); gamma-irradiation (0.4 kGy and 0.7 kGy); and refrigerated storage at 10 deg C e 90% of relative humidity for 2, 5, 10, 15, 20, 25, 30 and 35 days plus five days under environmental conditions to allow ripening and to simulate the product marketing. To evaluate the effects on fruit quality the following measurements were taken: - first experiment, disease incidence, skin color and loss of turgidity (as visual variables); skin and flesh color, weight loss, flesh firmness, total soluble solids, total titratable acidity, pH, lycopene and beta-carotene composition; - second experiment, sensorial analysis on samples submitted to the treatments with better results from the first experiment. Post harvest diseases were the main factor responsible for discarding fruits. Percentage of weight loss was higher on papayas stored without plastic packaging. Time affected the flesh firmness (F = 95%). However, there wasn't influence of the treatments on that parameter. A significant effect on skin color was observed with interaction between irradiation (0.4 and 0.7 kGy) and storage period. Once reached the edible stage, irradiated papayas presented more uniformity on skin color. For beta-carotene and Lycopene, the mean values were 1.27 to 1.79 and 19.16 to 23.90 mug/ml of flesh, respectively. But those substances weren't affected by the combined methods or the time. The same behavior were observed for total soluble solids, total titratable acidity and pH (mean values of 11.53 to 12.20 deg Brix, 0.117 to 0.136 g of citric acid/100g of flesh and 4.91 to 5.04, respectively). On sensory evaluation, the judges didn't get to detect significant differences among papayas submitted to different treatments. A synergism was verified on the techniques combination, with the best results obtained from the association of CP + PEabs + gamma-irradiation at 0.4 kGy, which reached a total storage period of 35 days. Thus, that is the post harvest hurdle technology recommend for exporting 'Golden' papayas to markets with quarantine restrictions to fruit-flies (author)

  6. Heat shock and gamma radiation in the quarantine process of Papaya CV. Solo(Carica papaya L.)

    Energy Technology Data Exchange (ETDEWEB)

    Pimentel, Rodrigo M.A.; Walder, Julio M.M. [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil)]. E-mails: rmapiment@cena.usp.br; jmwalder@cena.usp.br; Marcondes, Yvens E.M. [Universidade Metodista de Piracicaba (UNIMEP), SP (Brazil)]. E-mail: yvensmar@unimep.br

    2007-07-01

    Full text: To export, it is not only necessary to achieve quality standards, but also, quarantine standards. The main function of irradiation is quarantine control of fruit flies, therefore it is necessary to combine with treatments which focus at keeping fruit quality. The objective of this work was the evaluation of the quality of papaya submitted to heat treatment followed by irradiation at quarantine doses. Papayas were selected green and separated in treatments: a) control; irradiation at doses; b) 250, c) 500 Gy; d) hot water at 60 deg C for 30 seconds; and combined to doses e) 250, f) 500 Gy. The papayas were stored at 21 ({+-} 1 deg C) with relative humidity of 85 - 90%, and then after 8 days, they were evaluated by their soluble solid content, pH, titratable acidity, firmness, decay index and internal color. The analysis of mass loss and external color were executed at 0, 2, 4 and 7 days after irradiation. It was observed that in fruits submitted to hot water (d, e, f) there were less decay and in combined treatments (e, f) it was verified higher firmness. In the second day, irradiated treatments (b, c, e, f) were more yellow than the others. As to the other parameters evaluated, there were no differences between treatments (author)

  7. Heat shock and gamma radiation in the quarantine process of Papaya CV. Solo(Carica papaya L.)

    International Nuclear Information System (INIS)

    Full text: To export, it is not only necessary to achieve quality standards, but also, quarantine standards. The main function of irradiation is quarantine control of fruit flies, therefore it is necessary to combine with treatments which focus at keeping fruit quality. The objective of this work was the evaluation of the quality of papaya submitted to heat treatment followed by irradiation at quarantine doses. Papayas were selected green and separated in treatments: a) control; irradiation at doses; b) 250, c) 500 Gy; d) hot water at 60 deg C for 30 seconds; and combined to doses e) 250, f) 500 Gy. The papayas were stored at 21 (± 1 deg C) with relative humidity of 85 - 90%, and then after 8 days, they were evaluated by their soluble solid content, pH, titratable acidity, firmness, decay index and internal color. The analysis of mass loss and external color were executed at 0, 2, 4 and 7 days after irradiation. It was observed that in fruits submitted to hot water (d, e, f) there were less decay and in combined treatments (e, f) it was verified higher firmness. In the second day, irradiated treatments (b, c, e, f) were more yellow than the others. As to the other parameters evaluated, there were no differences between treatments (author)

  8. Potencial antifúngico de extractos de cuatro especies vegetales sobre el crecimiento de Colletotrichum gloeosporioides en papaya (Carica papaya en poscosecha

    Directory of Open Access Journals (Sweden)

    Nadia Landero Valenzuela

    2013-01-01

    Full Text Available El manejo de antracnosis por Colletotrichum gloeosporioides es el problema más importante en poscosecha de frutos tropicales. La actividad antifúngica de diferentes extractos vegetales fue evaluada in vitro e in vivo para controlar la antracnosis poscosecha en papaya. Extractos de ajo (Allium sativum (10 y 15 % y canela (Cinnamomum zeylanicum (0,0050; 0,0100; 0,0150 % mostraron efecto fungicida en contra de C. gloeosporioides para supresión de crecimiento micelial (100 %, inhibición de germinación (100 % y esporulación del hongo (100 %, considerándose estos extractos como los más promisorios para inhibir el desarrollo del hongo in vitro. Estudios in vivo también revelaron que los extracto de ajo (11,74 % y canela a dosis de 0,0054 % aplicada antes y al mismo tiempo de la inoculación con C. gloeosporioides, fueron las dosis óptimas para el control (severidad de la antracnosis en frutos de papaya artificialmente inoculados, mientras que a mayores dosis la severidad aumentó. La variable fitotoxicidad arrojó resultados consistentes con lo anterior, comprobándose que al aumentar las dosis de extracto de canela se presentó cambio de color en los frutos. Los resultados sugieren la posibilidad de usar estos extractos a dosis adecuadas como biofungicidas para controlar antracnosis en papaya en poscosecha.

  9. Potencial antifúngico de extractos de cuatro especies vegetales sobre el crecimiento de Colletotrichum gloeosporioides en papaya (Carica papaya en poscosecha

    Directory of Open Access Journals (Sweden)

    Nadia Landero Valenzuela

    2013-06-01

    Full Text Available El manejo de antracnosis por Colletotrichum gloeosporioides es el problema más importante en poscosecha de frutos tropicales. La actividad antifúngica de diferentes extractos vegetales fue evaluada in vitro e in vivo para controlar la antracnosis poscosecha en papaya. Extractos de ajo (Allium sativum (10 y 15 % y canela (Cinnamomum zeylanicum (0,0050; 0,0100; 0,0150 % mostraron efecto fungicida en contra de C. gloeosporioides para supresión de crecimiento micelial (100 %, inhibición de germinación (100 % y esporulación del hongo (100 %, considerándose estos extractos como los más promisorios para inhibir el desarrollo del hongo in vitro. Estudios in vivo también revelaron que los extracto de ajo (11,74 % y canela a dosis de 0,0054 % aplicada antes y al mismo tiempo de la inoculación con C. gloeosporioides, fueron las dosis óptimas para el control (severidad de la antracnosis en frutos de papaya artificialmente inoculados, mientras que a mayores dosis la severidad aumentó. La variable fitotoxicidad arrojó resultados consistentes con lo anterior, comprobándose que al aumentar las dosis de extracto de canela se presentó cambio de color en los frutos. Los resultados sugieren la posibilidad de usar estos extractos a dosis adecuadas como biofungicidas para controlar antracnosis en papaya en poscosecha.

  10. Evaluation of platelet augmentation activity of Carica papaya leaf aqueous extract in rats

    Directory of Open Access Journals (Sweden)

    Swati Patil

    2013-01-01

    Full Text Available Carica papaya leaves have been used traditionally to treat indigestion, as a vermifuge. Carica papayaleaves have also been shown to possess anti-tumor and immunomodulatory effects. The current study aims at determining the effect of Carica papaya leaves aqueous extract in increasing the platelet count in thrombocytopenic rat model. Aqueous extract of Carica papaya leaves at concentration of 400mg/kg and 800mg/kg were given to cyclophosphamide induced thrombocytopenic rats for a period of fifteen days. Blood was withdrawn at various time intervals to determine the platelet count. Also, the clotting time was determined on the 15th day of the study by capillary method. Carica papaya leaf extract was found to increase the platelet count and also to decrease the clotting time in rats. The study aims at determining the possible effects of papaya leaves in thrombocytopenia occurring in dengue fever.

  11. Tolerance induction to saline stress in papaya seeds treated with potassium nitrate and sildenafil citrate

    Directory of Open Access Journals (Sweden)

    Rafael Fonsêca Zanotti

    2013-12-01

    Full Text Available Papaya fruit (Carica papaya is a species of great commercial agriculture importance. Although has a saline stress sensitivity. Potassium nitrate (KNO3 and nitric oxide are substances that inducing saline stress tolerance. The aim of this study was evaluate the effects of sildenafil citrate (SC and KNO3 on the papaya seeds germination of under saline stress. Papaya seeds of hybrids Calimosa and JS12x Waimanalo were immersed in distilled water or KNO3 solutions and SC and followed germinated in distilled water or NaCl solution –0.3 MPa. Seeds of the hybrid JS12x Waimanalo had higher percentages of germination and normal seedlings. The controls of the two hybrids did not show saline stress tolerance during germination. Seeds of the hybrid JS12x Waimanalo, when treated with KNO3 or SC showed stress tolerance. The growth regulators tested were important in the induction of saline tolerance in papaya seeds.

  12. Histopathological changes in Wistar albino rats exposed to aqueous extract of unripe Carica papaya

    Directory of Open Access Journals (Sweden)

    Taofeeq Oduola

    2010-05-01

    Full Text Available Background: Exposure of animals to xenobiotics may or may not trigger adverse response at cellular levels. Aqueous extract of unripe Carica papaya is consumed by sickle cell patients as antisickling agent in Western Nigeria. Aim: This study was undertaken to investigate the effects of Carica papaya on certain organs in Wister albino rats exposed to aqueous extract of unripe Carica papaya. Materials and Methods: Different doses of aqueous extract of unripe Carica papaya were administered orally daily for 42 days to six groups of rats. At the end of exposure, the animals were sacrificed and tissue sections were prepared from livers, kidneys, hearts and small intestines using standard techniques. Results: Histopathological results showed that no pathological changes were observed in tissue sections of experimental animals when compared with tissue sections of the same organs in control animals. Conclusion: No pathological changes were elicited in the organs of rats exposed to aqueous extract of unripe Carica papaya.

  13. Evaluation of Antimicrobial Properties of Edible Surface Coating Based on Carrageenan Conjugated with Silver Nanoparticles on Sekaki Papaya (Carica Papaya cv. Sekaki): A New Antimicrobial Edible Coating

    International Nuclear Information System (INIS)

    Antibacterial properties of edible surface coating based on carrageenan incorporated with silver nanoparticles (SNPs), was investigated against Escherichia coli and Staphylococcus aureus to obtain optimum concentration of SNPs. Results obtained indicate that SNPs with concentration of 40 ml L-1 effectively inhibited the growth of both Gram negative and Gram positive bacteria. Sekaki papaya fruits were then coated with prepared edible coating formulation comprising of carrageenan (0.8 % w/v) and glycerol (1.0 % w/v) with and without SNPs (40 ppm) and stored at ambient conditions (26±2 degree Celsius and 60±10 % RH). Microbial analysis of coated and uncoated papaya samples during storage indicated that the edible coating comprising of carrageenan, glycerol and SNPs, strongly inhibited the growth of fungus that caused post harvest diseases of papaya as compared to uncoated and coated papaya fruits with edible coating without SNPs. (author)

  14. Implementación del enfoque de derechos humanos: la reforma sanitaria chilena

    Directory of Open Access Journals (Sweden)

    Nuria Cunill-Grau

    2014-01-01

    Full Text Available EI propósito de este trabajo es analizar la incidencia de la arquitectura institucional publica en la implementación de las políticas públicas con enfoque de derechos humanos. Se adopta como caso de estudio la reforma sanitaria chilena a partir del año 2005, que reconoce garantías exigibles de acceso, oportunidad, calidad y protección financiera para un conjunto de problemas de salud a la ciudadanía, independientemente de su adscripción al sistema público 0 privado. La metodología integró un análisis cualitativo de normas legales, instrumentos de gestión y entrevistas en profundidad principalmente a directivos de salud. Los resultados muestran que, no obstante los evidentes logros de la reforma chilena en pos de la equidad en salud, resultan limitados sus esfuerzos de dotar de gobernanza sistémica, accountability y espacio público a su arquitectura institucional, comprometiendo con ello su intención de instaurar un enfoque de derechos humanos. Se concluye que el patrón hegemónico de diseño institucional, organización y gestión de los servicios sociales requiere ser enfrentado para construirviabilidad a la implementación de este tipo de política y se sugiere la importancia de adoptar estrategias diferenciadas de accountabitily que consideren las asimetrías sociales en la apropiación y exigibilidad de los derechos.

  15. Optical and ultrastructural study of the pollen grain development in hermaphrodite papaya tree (Carica papaya L.)

    Scientific Electronic Library Online (English)

    Lídia Márcia Silva, Santos; Telma Nair Santana, Pereira; Margarete Magalhães de, Souza; Pedro Correa, Damasceno Junior; Fabiane Rabelo da, Costa; Beatriz Ferreira, Ribeiro; Noil Gomes de, Freitas; Messias Gonzaga, Pereira.

    2008-06-01

    Full Text Available Esta pesquisa descreve os estádios de formação do gameta masculino (grão de pólen) em mamoeiro. Para isto foram coletados botões florais em diferentes etapas de desenvolvimento e as anteras foram tratadas quimicamente para serem observadas ao microscópio óptico e eletrônico de transmissão. O desenvo [...] lvimento do grão de pólen seguiu o padrão normal para Angiospermas. Foram descritos os estádios de desenvolvimento do grão de pólen desde meiócitos ou células mães de micrósporos até o grão de pólen binucleado. Na microesporogênese, as células esporogênicas sofreram meiose dando origem as tétrades, com posterior liberação dos micrósporos da tétrade. A microgametogênese caracterizou-se, principalmente, pela divisão mitótica assimétrica de cada micrósporo originando grãos de pólen binucleados. Estruturas semelhantes a plastídios foram encontradas no citoplasma da célula generativa e agregados próximos ao núcleo da célula generativa. Observou-se degeneração gradativa das células do tapete no decorrer da formação do gameta. Abstract in english The objective of this study was to describe the pollen grain development in hermaphrodite papaya tree. The flower buds were collected at different stages of the development and the anthers were treated chemically for observation under optical and electronic transmission microscopes. The pollen grain [...] development followed the normal pattern described for the Angiosperms. The pollen grain development was described from meiocyte to the mature pollen grain. In the microsporogenesis, the microspore mother cells or the meiocytes underwent meiosis giving rise to the tetrads that were enclosed by the calose. Later, the tetrads were released by the dissolution of the calose by calase activity and microspores underwent mitosis. Microgametogenesis was characterized by asymmetrical mitotic division of each microspore giving rise to bi-nucleate pollen grains. The structures similar to the plastids were found in the cytoplasm and close to the nucleus of the generative cell. Gradual degeneration was observed in the tapetum during the male gamete development.

  16. Comportamiento mecánico de frutos de papaya bajo compresión axial / Mechanical behavior of papaya fruits under axial compression

    Scientific Electronic Library Online (English)

    Enrique, Vázquez García; Horacio, Mata Vázquez; Rafael, Ariza Flores; Felipe, Santamaría Basulto; Irán, Alia Tejacal.

    2013-12-01

    Full Text Available Los frutos de papaya (Caricapapaya L.) son muy susceptibles a los daños mecánicos, lo que repercute en fuertes pérdidas en poscosecha. El objetivo del presente trabajo fue evaluar el comportamiento mecánico de frutos de papaya en poscosecha, para lo cual se realizaron dos experimentos en el Campo Ex [...] perimental Las Huastecas, INIFAP durante los años 2011 y 2012. Las variables evaluadas fueron la fuerza y la deformación requeridas por los frutos para alcanzar el límite elástico, el punto de biocedencia y el punto de ruptura. La compresión se realizó con un Instron Universal 4460. En el primer experimento se evaluaron frutos en madurez de consumo tratados en pre-cosecha con cinco combinaciones de fertilización; los tratamiento N-P-K-Cu-Zn, N-P-K-Ca-Zn, N-P-K y N-P-K-Fe-Zn registraron mayor resistencia a la fuerza de compresión y resultaron diferentes al tratamiento N-P-K-Mn-Zn (Tukey, p? 0.05). En el segundo experimento se evaluaron tres cultivares en dos estados de madurez; los frutos en madurez fisiológica fueron más resistentes para alcanzar el límite elástico (414 N), con relación a los frutos en madurez de consumo (67 N), con diferencias estadísticas (Tukey, p? 0.05). Los frutos del híbrido PK 02 requirieron mayor fuerza para alcanzar el límite elástico (298 N), con relación a los frutos de la variedad Maradol roja (250 N) y del híbrido PK 03 (173 N), con diferencias (Tukey, p? 0.05). Se concluye que los frutos de papaya tuvieron un comportamiento mecánico diferente, en función del manejo nutricional, del tipo de cultivar y del estado de madurez. Abstract in english The fruits of papaya (Carica papaya L.) are very susceptible to mechanical damage, which results in heavy losses in post-harvest. The aim of this study was to evaluate the mechanical behavior of papaya fruits in post-harvest, for which two experiments were conducted in the Experimental Field The Hua [...] steca, INIFAP during the years 2011 and 2012. The variables evaluated were the strength and deformation required for the fruit to reach the elastic limit, biocedence and the breaking point. The compression was performed with a Instron Universal 4460. In the first experiment for ripening fruit treated with pre-harvest five combinations of fertilization, the treatment N-P-K-Cu-Zn, N-P-K-Ca-Zn, N-P-K-Fe-Zn reported increased resistance to force compression and was different to the treatment N-P-K-Mn-Zn (Tukey, p? .05). In the second experiment three cultivars were evaluated in two states of maturity, the fruits at physiological maturity were more resistant to attain the elastic limit (414 N) in relation to ripening (67 N), with statistical differences (Tukey, p? .05). The fruits of the hybrid PK 02 required more force to achieve the yield strength (298 N) with respect to the fruits of the variety Maradol roja (250 N) and the hybrid PK 03 (173 N), with differences (Tukey, p? .05). It is concluded that papaya fruits had different mechanical behavior depending on the nutritional management, the type of cultivar and maturity.

  17. An analysis on DNA fingerprints of thirty papaya cultivars (Carica papaya L.), grown in Thailand with the use of amplified fragment length polymorphisms technique.

    Science.gov (United States)

    Ratchadaporn, Janthasri; Sureeporn, Katengam; Khumcha, U

    2007-09-15

    The experiment was carried out at the Department of Horticulture, Ubon Ratchathani University, Ubon Ratchathani province, Northeast Thailand during June 2002 to May 2003 aims to identify DNA fingerprints of thirty papaya cultivars with the use of Amplified Fragment Length Polymorphisms (AFLP) technique. Papaya cultivars were collected from six different research centers in Thailand. Papaya plants of each cultivar were grown under field conditions up to four months then leaf numbers 2 and 3 of each cultivar (counted from top) were chosen for DNA extraction and the samples were used for AFLP analysis. Out of 64 random primers being used, 55 pairs gave an increase in DNA bands but only 12 pairs of random primers were randomly chosen for the final analysis of the experiment. The results showed that AFLP markers gave Polymorphic Information Contents (PIC) of three ranges i.e., AFLP markers of 235 lied on a PIC range of 0.003-0.05, 47 for a PIC range of 0.15-0.20 and 12 for a PIC range of 0.35-0.40. The results on dendrogram cluster analysis revealed that the thirty papaya cultivars were classified into six groups i.e., (1) Kaeg Dum and Malador (2) Kaeg Nuan (3) Pakchong and Solo (4) Taiwan (5) Co Coa Hai Nan and (6) Sitong. Nevertheless, in spite of the six papaya groups all papaya cultivars were genetically related to each other where diversity among the cultivars was not significantly found. PMID:19090101

  18. An Analysis on DNA Fingerprints of Thirty Papaya Cultivars (Carica papaya L., Grown in Thailand with the Use of Amplified Fragment Length Polymorphisms Technique

    Directory of Open Access Journals (Sweden)

    Janthasri Ratchadaporn

    2007-01-01

    Full Text Available The experiment was carried out at the Department of Horticulture, Ubon Ratchathani University, Ubon Ratchathani province, Northeast Thailand during June 2002 to May 2003 aims to identify DNA fingerprints of thirty papaya cultivars with the use of Amplified Fragment Length Polymorphisms (AFLP technique. Papaya cultivars were collected from six different research centers in Thailand. Papaya plants of each cultivar were grown under field conditions up to four months then leaf numbers 2 and 3 of each cultivar (counted from top were chosen for DNA extraction and the samples were used for AFLP analysis. Out of 64 random primers being used, 55 pairs gave an increase in DNA bands but only 12 pairs of random primers were randomly chosen for the final analysis of the experiment. The results showed that AFLP markers gave Polymorphic Information Contents (PIC of three ranges i.e., AFLP markers of 235 lied on a PIC range of 0.003-0.05, 47 for a PIC range of 0.15-0.20 and 12 for a PIC range of 0.35-0.40. The results on dendrogram cluster analysis revealed that the thirty papaya cultivars were classified into six groups i.e., (1 Kaeg Dum and Malador (2 Kaeg Nuan (3 Pakchong and Solo (4 Taiwan (5 Co Coa Hai Nan and (6 Sitong. Nevertheless, in spite of the six papaya groups all papaya cultivars were genetically related to each other where diversity among the cultivars was not significantly found.

  19. Evaluación de la Felicidad: Análisis Psicométrico de la Escala de Felicidad Subjetiva en Población Chilena

    Directory of Open Access Journals (Sweden)

    Pablo Vera-Villarroel

    2011-01-01

    adecuadas y altamente signifi cativas en la línea de lo esperado teóricamente. La evidencia indica adecuación de la escala para su utilización en la evaluación de este constructo en población chilena.

  20. Estudo e modelagem da cinética de desidratação osmótica do mamão formosa (Carica papaya L.) / Kinetics and modeling on osmotic dehydration of papaya (Carica papaya L.)

    Scientific Electronic Library Online (English)

    Ânoar Abbas, El-Aquar; Fernanda E. Xidieh, Murr.

    2003-04-01

    Full Text Available O presente trabalho teve como objetivo o estudo da cinética e modelagem do processo de desidratação osmótica de cubos de mamão Formosa (Carica papaya L.), assim como da qualidade do produto final. O tratamento osmótico foi conduzido a 30ºC, com agitação de 110rpm, utilizando-se dois tipos de soluçõe [...] s de sacarose 70ºBrix: a primeira contendo lactato de sódio 2,4% p/p e ácido láctico 0,1M e a segunda com lactato de sódio 2,4% p/p e ácido cítrico 0,1M. O estudo da cinética de desidratação osmótica mostrou que a solução contendo ácido cítrico apresentou valor de perda de água (WL), ao final de 48 horas de processo, ligeiramente superior ao encontrado para a solução contendo ácido láctico. Comportamento contrário ocorreu para o ganho de sólidos (SG). O ajuste dos dados experimentais foi realizado através do modelo difusional para geometria cúbica, sem considerar encolhimento durante o processo e um modelo empírico de dois parâmetros. Os coeficientes de difusividade efetiva de água (Def) variaram de 1,27 a 5,03 x 10-10 m²/s. A qualidade da fruta após processamento foi avaliada através de análises de vitamina C, carotenóides totais, acidez e pH. Abstract in english The present work had as objective the study of kinetics and mathematical modeling of osmotic dehydration process of papaya cubes (Carica papaya L.), as well as the quality of the final product. The osmotic treatment was carried out for 30ºC, with agitation 110rpm, with two types of 70ºBrix sucrose s [...] olutions: solution containing sodium lactate 2,4% w/w and lactic acid 0,1M and another one with sodium lactate 2,4% w/w and citric acid 0,1M. The study of kinetics of osmotic dehydration showed the solution containing citric acid had a water loss (WL) value, at the end of 48 hours of process, lightly superior to the found for the solution containing lactic acid. Opposite behavior happened for the solid gain (SG). Modeling of experimental data was accomplished through the difusional model for cubic geometry, considering no shrinkage during the process and an empirical two parameters one. The coefficients of effective difusivity of water (Def) varied from 1,27 to 5,03 x 10-10 m²/s. Quality of fruit after processing was evaluated through vitamin C, total carotenoid, acidity and pH analyses.

  1. Preferential reproduction mode of hermaphrodite papaya plant (Carica papaya L; Caricaceae) / Modo de reprodução preferencial de plantas hermafroditas de mamoeiro (Carica papaya L; Caricaceae)

    Scientific Electronic Library Online (English)

    Pedro Corrêa, Damasceno junior; Telma Nair Santana, Pereira; Messias Gonzaga, Pereira; Francisco Filho da, Silva; Margarete de Magalhães, Souza; Rodrigo Gualandi, Nicoli.

    2009-03-01

    Full Text Available Esta pesquisa teve o objetivo de estudar o sistema reprodutivo preferencial de plantas hermafroditas de mamoeiro, com base na natureza histoquímica dos grãos de pólen, receptividade do estigma, teste de germinação in vivo do grão de pólen e razão pólen : óvulo. Na análise histoquímica dos grãos de p [...] ólen, foram utilizados os corantes Sudan IV e solução de I2KI; a receptividade do estigma foi avaliada com a solução de acetato de alfa-naftil em flores abertas e fechadas. A germinação e o crescimento do tubo polínico in vivo foram avaliados em flores fechadas, utilizando solução de azul de anilina a 0,1%. Para estimar a razão pólen:óvulo, anteras de cada flor foram dissecadas, e os grãos de pólen foram contados; ovários foram dissecados, e os óvulos foram contados sob estereomicroscópio. Os resultados indicaram que os grãos de pólen do mamoeiro são de natureza lipídica; os estigmas estavam receptivos antes da abertura e até 48 h após a abertura; os grãos de pólen germinaram e emitiram tubo polínico antes da abertura floral, e a relação pólen:óvulo, indicou predominância do sistema reprodutivo autógamo. Esses resultados indicaram que o modo de reprodução preferencial do mamoeiro hermafrodita é autógamo facultativo com cleistogamia. Abstract in english This research was done to study the reproductive system of papaya hermaphrodite plant based on the histochemical nature of pollen grain, stigma receptivity, in vivo pollen grain germination and pollen:ovule ratio. In the histochemical analysis, pollen grains were stained by using Sudan IV and I2KI s [...] olution ; the stigma receptivity was assessed by alpha-naphthtyl acetate solution in closed and opened flowers. Pollen germination and pollen tube growing were examined in flower buds near anthesis with 0.1% aniline blue. To estimate the pollen:ovule ratio , anthers from each flower bud were dissected and all pollen grains were counted; ovules were dissected from ovaries and were counted under stereomicroscope. The results indicated that papaya pollen grains are of lipidic nature; the stigmas were receptive before the opening and until 48 hours after opening; the pollen grains germinated and emitted polinic tube before flower opening and the pollen:ovule ratio indicated the predominance of autogamous reproductive system. These results indicate that hermaphrodite papaya trees is preferentially of optional autogamous with cleistogamy.

  2. Starch edible coating of papaya: effect on sensory characteristics

    Directory of Open Access Journals (Sweden)

    Ariane Castricini

    2012-03-01

    Full Text Available The coating of papayas with Cassava Starch (CS and carboxymethyl starch (CMS is an alternative to extend the shelf life of these fruits. This study evaluated the effect of the three different levels of CS and CMS (1, 3, and 5% on sensory characteristics of papayas during storage. Nine selected and trained assessors evaluated 13 sensory attributes using the Multiple Comparison Test. The appearance and flavor attributes of the papayas treated with CS and CMS were compared to the control or reference sample (R - fruit without coating using a nine-point scale, which varied from 1: less intense than R; 5: equal to R; 9: more intense than R. The samples were coded with three digit numbers and evaluated with repetition by a panel of assessors. In general, appearance was more affected by the coatings than flavor. Fruits coated with 3 and 5% of both coatings kept the green color longer than the other coating’s concentrations, and at 5% the color of the fruits was less uniform on the last evaluation day. The 3 and 5% CS coating gave greater brightness to the fruits. 5% CMS favored the presence of fungi and damaged the fruit surface at the 14th day of storage. The CS coating at 5% presented peeled surface during all experimental time. Changes in fruits’ flavor were perceived at the 12th and 14th days of storage. A less characteristic flavor and a bitter taste were noticed in the fruits coated with CS and CMS at 5% at the 12th day of storage.

  3. Papaya Extract to Treat Dengue: A Novel Therapeutic Option?

    OpenAIRE

    N. Sarala; Paknikar, SS

    2014-01-01

    Dengue is a viral disease that today affects a vast number of people in over 125 countries and is responsible for a sizable number of deaths. In the absence of an effective antiviral drug to treat the disease, various treatments are being investigated. Studies have indicated that the juice of the leaves of the Carica papaya plant from the family Caricaceae could help to increase the platelet levels in these patients. This review describes some of the published studies on this topic. The searc...

  4. Relaxation phenomena of radicals induced in irradiated fresh papayas

    International Nuclear Information System (INIS)

    Electron spin resonance spectrometry of the ?-irradiated fresh papayas followed by freeze-drying and powderization was performed. We found a strong single peak in the flesh was observed at g=2.004 and attributed to organic free radicals. Using the method of Lund et al., relaxation times of the peak from 0 to 14 days-stored samples after ?-irradiation were calculated. T2 showed a dose response, while T1 kept almost constant by the increment of doses. The ?-radiation-induced radicals showing progressive saturation behaviors can be caused through a different pathway from indirect effects by the low LET radiations. (author)

  5. INTERACCIÓN DE HONGOS MICORRÍZICOS ARBUSCULARES Y FERTILIZACIÓN FOSFATADA EN PAPAYA

    Directory of Open Access Journals (Sweden)

    Evangelina Esmeralda Qui\\u00F1ones-Aguilar

    2012-01-01

    Full Text Available Los estudios realizados sobre la simbiosis que forman los hongos micorrízicos arbusculares (HMA con diversas plantas, han revelado la importancia de estos endófitos en distintos aspectos relacionados con su nutrición y protección. Así, para la producción de frutales cultivados en vivero, el uso de HMA es una alternativa para obtener plantas más sanas y vigorosas en menor tiempo para su establecimiento en campo. En los suelos, el fósforo (P no se encuentra fácilmente disponible para las plantas, por lo que éstas han desarrollado algunas estrategias para absorberlo, como: cambios morfológicos, bioquímicos y moleculares en la raíz, además de establecer asociaciones con HMA, los cuales por medio de sus hifas promueven la absorción y transporte del P. En la presente investigación se evaluó la respuesta de papaya (Carica papaya L. a la inoculación con la cepa de HMA Glomus sp. Zac-2 y con fertilización fosfatada. Se estableció un experimento trifactorial mixto (2 × 3 × 4. Los factores estudiados fueron: inoculación micorrízica, fuente y dosis de P. Se evaluaron las variables: altura de planta (AP, diámetro de tallo (DT, área foliar (AF, volumen radical (VR, peso seco radical (PSR, peso seco de follaje (PSF y porcentaje de colonización micorrízica (PCM. Los resultados obtenidos mostraron diferencias significativas (Tukey, P £ 0.05; las plantas inoculadas mostraron incrementos en crecimiento con respecto a las plantas sin inocular de 486.51% en AP; 594.31% en DT; 1084.61% en AF; 6962.35% en VR; 13591.43% en PSR y 4992.03% en PSF. La respuesta de las plantas a la fertilización fue variable y ésta dependió de la dosis y fuente de P y de la interacción de ambos factores con los HMA. Los resultados mostraron que las plantas de papaya responden positivamente a estos microorganismos, lo que les permite aprovechar mejor el P disponible del suelo y con ello favorecer su adaptación en campo. Esto sugiere que los HMA son benéficos, en condiciones de sustrato libre de otros microorganismos, durante la etapa de producción de plántulas de papaya en vivero.

  6. Empleo del método de secado convectivo combinado para la deshidratación de papaya (Carica papaya L.), variedad Maradol roja / Use of convective dry method combined for dehydration of papaya fruit (Carica papaya L.), variety Maradol roja

    Scientific Electronic Library Online (English)

    Sahylin, Muñiz Becerá; Antihus, Hernández Gómez; Annia, García Pereira; Lilia, Méndez Lagunas.

    Full Text Available La presente investigación tiene como objetivo evaluar el método de secado convectivo de papaya (Carica papaya L.) variedad Maradol roja, combinado con la aplicación de pretratamientos de osmosis (DOSC) y escaldado simple (ESSC), mediante el efecto de los factores tecnológicos del secador: temperatur [...] a (40 y 60 ºC) y velocidad del flujo de aire (2,5 y 1,5 m/s) sobre el comportamiento de las propiedades de calidad de la fruta deshidratada y la cinética del proceso. El pretratamiento de escaldado simple se realizó en agua destilada caliente a 70°C durante 15 minutos y la deshidratación osmótica u osmosis a 60 °C por 4 horas a frutas cortadas en cubos de 1,5± 0,2 cm de largo por 1,0±0,01 cm de espesor, utilizando una solución de sacarosa comercial a 50°Brix. Como diseño experimental se empleó un Diseño Factorial Completo 2² y para el procesamiento estadístico de los datos el software STATGRAPHICS Plus 5.1. Como principales resultados se obtuvo que en ambos procesos el aumento de la temperatura a 60 ºC y la reducción de la velocidad del flujo de aire a 1,5 m/s disminuyó el tiempo de secado con valores de R² >0,95 y ? Abstract in english This research aim is to evaluate the convective dry method of papaya fruit (Carica papaya L.), variety Maradol roja, combined with simply blanching (ESSC) and Osmosis (DOSC) pre-treatments by the effect of technological factors of dryer: temperature (40 y 60 ºC) and air flow speed (2,5 y 1,5 m/s) ab [...] ove the behavior of quality properties in dehydrated fruit and the process´s kinetic. The simple blanching was realized using hot water 70 °C per 15 min and the osmotic dehydration 60 °C per 4 h to the cubes with 1,5±0,2 cm length per 1,0 ± 0,01 cm de thickness, using a sucrose osmotic solution at 50 °Brix concentration. As experimental design was used a Full Factorial 2² Design and the STATGRAPHICS Plus 5.1 software. As principal results was obtained that in both process the higher temperature to 60 ºC and lower air flow speed to 1,5 m/s provokes a reduction on drying time with value of R² >0,95 y ?

  7. Presença dos vírus da mancha anelar e do amarelo letal em frutos de mamoeiro comercializados The presence of Papaya ringspot virus and Papaya lethal yellowing virus in commercialized papaya fruits

    Directory of Open Access Journals (Sweden)

    Najara F. Ramos

    2008-12-01

    Full Text Available Considerando os índices elevados de incidência de vírus que infetam o mamoeiro (Carica papaya em condições de campo e seus sintomas típicos nos frutos, foi efetuado um levantamento na Central de Comercialização do Estado do Ceará (CEASA de frutos infetados com o vírus da mancha anelar do mamoeiro (Papaya ringspot virus, PRSV e o vírus do amarelo letal do mamoeiro (Papaya lethal yellowing virus, PLYV. Os principais Centros de comercialização de frutos são as CEASAs e os produtos comercializados nos supermercados são, em grande parte, adquiridos nesses Centros. Os frutos coletados aleatoriamente nas bancas de comercialização da CEASA foram levados ao Laboratório de Virologia Vegetal, da Universidade Federal do Ceará (UFC para análises sintomatológicas e testes de "enzyme linked immunosorent assay" (ELISA indireto, com anti-soros específicos para o PRSV e PLYV. De acordo com os resultados das análises sintomatológicas, confirmadas por sorologia, dentre os 8.400 frutos analisados, 5,5% estavam infetados por PRSV e 1,2% por PLYV, demonstrando a necessidade de implementar um programa rigoroso de controle, envolvendo erradicação de fontes de vírus no campo, representados por plantas infetadas nos pomares, áreas abandonadas e inclusive cultivos de fundo de quintal.Considering the high incidence of virus in papaya (Carica papaya orchards and their typical symptoms in the fruits, a survey was carried out in the Central Market in the State of Ceará (CEASA for the presence of papaya fruits infected with Papaya ringspot virus (PRSV and Papaya lethal yellowing virus (PLYV. CEASAs are the most important fruit commercial centers, and a great number of plant products sold in supermarkets are obtained in CEASA. Fruits were randomly collected from CEASA stalls and brought to the Plant Virus Laboratory at the Federal University of Ceará (UFC to be analyzed for typical virus symptoms and tested by indirect enzyme linked immunosorbent assay (ELISA against antisera specific to PRSV and PLYV. According to the results obtained by symptom analysis, which were confirmed by serology, among the 8,400 fruits analyzed 5.5% were infected with PRSV and 1.2% with PLYV, showing the need to implement a control program involving eradication of virus sources in the field, represented by infected plants in papaya orchards, abandoned orchards and infected plants identified in backyards.

  8. Presença dos vírus da mancha anelar e do amarelo letal em frutos de mamoeiro comercializados / The presence of Papaya ringspot virus and Papaya lethal yellowing virus in commercialized papaya fruits

    Scientific Electronic Library Online (English)

    Najara F., Ramos; Aline K.Q., Nascimento; Maria Fátima B., Gonçalves; José Albérsio A., Lima.

    2008-12-01

    Full Text Available Considerando os índices elevados de incidência de vírus que infetam o mamoeiro (Carica papaya) em condições de campo e seus sintomas típicos nos frutos, foi efetuado um levantamento na Central de Comercialização do Estado do Ceará (CEASA) de frutos infetados com o vírus da mancha anelar do mamoeiro [...] (Papaya ringspot virus, PRSV) e o vírus do amarelo letal do mamoeiro (Papaya lethal yellowing virus, PLYV). Os principais Centros de comercialização de frutos são as CEASAs e os produtos comercializados nos supermercados são, em grande parte, adquiridos nesses Centros. Os frutos coletados aleatoriamente nas bancas de comercialização da CEASA foram levados ao Laboratório de Virologia Vegetal, da Universidade Federal do Ceará (UFC) para análises sintomatológicas e testes de "enzyme linked immunosorent assay" (ELISA) indireto, com anti-soros específicos para o PRSV e PLYV. De acordo com os resultados das análises sintomatológicas, confirmadas por sorologia, dentre os 8.400 frutos analisados, 5,5% estavam infetados por PRSV e 1,2% por PLYV, demonstrando a necessidade de implementar um programa rigoroso de controle, envolvendo erradicação de fontes de vírus no campo, representados por plantas infetadas nos pomares, áreas abandonadas e inclusive cultivos de fundo de quintal. Abstract in english Considering the high incidence of virus in papaya (Carica papaya) orchards and their typical symptoms in the fruits, a survey was carried out in the Central Market in the State of Ceará (CEASA) for the presence of papaya fruits infected with Papaya ringspot virus (PRSV) and Papaya lethal yellowing v [...] irus (PLYV). CEASAs are the most important fruit commercial centers, and a great number of plant products sold in supermarkets are obtained in CEASA. Fruits were randomly collected from CEASA stalls and brought to the Plant Virus Laboratory at the Federal University of Ceará (UFC) to be analyzed for typical virus symptoms and tested by indirect enzyme linked immunosorbent assay (ELISA) against antisera specific to PRSV and PLYV. According to the results obtained by symptom analysis, which were confirmed by serology, among the 8,400 fruits analyzed 5.5% were infected with PRSV and 1.2% with PLYV, showing the need to implement a control program involving eradication of virus sources in the field, represented by infected plants in papaya orchards, abandoned orchards and infected plants identified in backyards.

  9. LOS DERECHOS SOCIALES DE PRESTACIÓN EN LA JURISPRUDENCIA CHILENA

    Directory of Open Access Journals (Sweden)

    José Ignacio Martínez Estay

    2010-01-01

    Full Text Available Parte importante de los derechos sociales consisten en prestaciones. Desde el punto de vista jurídico ello parece ser una limitante para su garantía y satisfacción, pues dependen de los recursos económicos de que disponga el Estado. Por eso, lo usual es que si se constitucionalizan no llevan aparejada una garantía jurisdiccional. Así ocurre en la Constitución chilena. Dicha limitación se ve ratificada por la jurisprudencia de la Corte Suprema, que reiteradamente ha negado lugar a recursos de protección en que se intentaba la tutela de derechos sociales consistentes en prestaciones, vinculándolos a algún derecho protegido jurisdiccionalmente. Pero la reciente jurisprudencia del Tribunal Constitucional respecto del derecho a la protección de la salud y del derecho a la seguridad social contradice lo anterior, atribuyéndole a los derechos sociales consistentes en prestaciones el mismo valor que el de los demás derechos y libertades.

  10. Nueva metodología para un ranking de Universidades Chilenas

    Directory of Open Access Journals (Sweden)

    David Rappoport

    2006-12-01

    Full Text Available Este artículo cuestiona la metodología tradicional de elaboración de rankings de universidades y sugiere la elaboración de tablas de posiciones de universidades chilenas construidas sobre la base del ingreso de sus titulados, verificándose su validez estadística.Así, el concepto de “ranking” se acota al de categoría: universidades de primera, segunda y tercera clase. Los resultados arrojan las siguientes conclusiones: primero, existen importantes diferencias en el ingreso entre los titulados de las distintas universidades; segundo, el ranking y la magnitud de los diferenciales medios de ingresos dependen de la carrera considerada, reafirmando la necesidad de elaborar rankings separadamente por carrera, y, tercero, se observa que existe un premio por reputación entre tradicionalesvs. derivadas, y entre privadas antiguas vs. privadas nuevas (para aquellas ubicadas en la Región Metropolitana y V Región.

  11. Política educativa chilena 1965-2009. ¿Qué oculta esa trama?

    Directory of Open Access Journals (Sweden)

    María Angélica Oliva

    2010-01-01

    Full Text Available Se aborda la política educativa chilena del período 1965-2009, inquiriéndose, ¿Qué oculta la trama? La conjetura es, la política educativa, a excepción del gobierno de Allende, muestra la institución del orden neoliberal. Se revisan algunas antinomias, mecanismos de ese orden, que se introduce en1965 con la pedagogía por objetivos y la teoría del capital humano. Esta pedagogía, es profundizada en dictadura, junto a la idea de subsidiariedad, que impulsa el cambio del rol del Estado, regulado en la Constitución de 1980, aún vigente, y presente en la Ley General de Educación. La municipalización, la transformación en el financiamiento y la pérdida del estatus de funcionario público del profesorado, son un legado de la dictadura. Ello, desperfila el afán democrático de la actual política educativa, transformándola en cómplice de un sistema que genera desigualdad y fragmentación social.

  12. High-density linkage mapping revealed suppression of recombination at the sex determination locus in papaya.

    OpenAIRE

    Ma, Hao; Moore, Paul H.; LIU, Zhiyong; Kim, Minna S; Yu, Qingyi; Fitch, Maureen M M; Sekioka, Terry; Andrew H. Paterson; Ming, Ray

    2004-01-01

    A high-density genetic map of papaya (Carica papaya L.) was constructed using 54 F(2) plants derived from cultivars Kapoho and SunUp with 1501 markers, including 1498 amplified fragment length polymorphism (AFLP) markers, the papaya ringspot virus coat protein marker, morphological sex type, and fruit flesh color. These markers were mapped into 12 linkage groups at a LOD score of 5.0 and recombination frequency of 0.25. The 12 major linkage groups covered a total length of 3294.2 cM, with an ...

  13. Phenol induced by irradiation does not impair sensory quality of fenugreek and papaya

    International Nuclear Information System (INIS)

    The effect of radiation processing on the sensory quality of fenugreek and papaya exposed to doses in the range of 2.5–10 kGy and 100 Gy–2.5 kGy respectively was investigated. Despite an increase in the content of phenol in the volatile oil of these food products overall sensory quality of the irradiated and control samples was not significantly affected by radiation processing. - Highlights: • Sensory quality of fenugreek and papaya is not significantly affected by radiation processing. • Irradiation did not adversely affect the overall acceptability of the processed samples. • Radiation processing was thus successfully applied to the preservation of fenugreek and papaya

  14. Papaya pulp gelling: is it premature ripening or problems of water accumulation in the apoplast?

    Directory of Open Access Journals (Sweden)

    Jurandi Gonçalves de Oliveira

    2010-12-01

    Full Text Available Gelled aspect in papaya fruit is typically confused with premature ripening. This research reports the characterization of this physiological disorder in the pulp of papaya fruit by measuring electrolyte leakage, Pi content, lipid peroxidation, pulp firmness, mineral contents (Ca, Mg and K - in pulp and seed tissues, and histological analysis of pulp tissue. The results showed that the gelled aspect of the papaya fruit pulp is not associated with tissue premature ripening. Data indicate a reduction of the vacuole water intake as the principal cause of the loss of cellular turgor; while the waterlogged aspect of the tissue may be due to water accumulation in the apoplast.

  15. Viabilidade polínica de Carica papaya L.: uma comparação metodológica / Pollen viability of Carica papaya L.: a methodological comparison

    Scientific Electronic Library Online (English)

    Márcia, Munhoz; Cynthia Fernandes Pinto da, Luz; Paulo Ernesto, Meissner Filho; Orthud Monika, Barth; Fernanda, Reinert.

    2008-06-01

    Full Text Available A viabilidade polínica do mamoeiro cultivar 'Sunrise Solo' (Carica papaya L.) foi estudada utilizando germinação in vitro e testes colorimétricos, assim como a validade dos testes colorimétricos como estimativa de viabilidade comparada àquela do teste germinativo. Os dois meios de cultura, descritos [...] na literatura como meios eficientes para germinação da espécie, diferem basicamente pela presença de nutrientes essenciais e concentração de ágar. O meio de cultura sem elementos essenciais e com maior concentração de ágar forneceu o melhor índice de germinação polínica (65%). Os cinco corantes testados foram: 2,3,5-cloreto de trifeniltetrazólio (TTC), Alexander, carmim acético, lugol e Sudan IV. O teste de coloração com TTC forneceu estimativa de viabilidade (67,5%) equivalente ao teste de germinação in vitro e, portanto, confiável de viabilidade polínica. Os demais corantes testados superestimaram a viabilidade polínica (> 90%), porém são eficientes na determinação de constituintes celulares e da integridade do grão de pólen. Abstract in english Pollen viability of Carica papaya L. 'Sunrise Solo' cultivar was estimated using in vitro germination and pollen stain tests. Two culture media described in the literature as efficient for pollen in vitro germination of C. papaya were used. Germination was higher in the media without essential eleme [...] nts but with a higher concentration of agar (65% compared with 51.5%). These results were compared with viability estimates based on staining pollen. The five stains used to test for pollen viability were: 2,3,5-triphenyl tetrazolium chloride - TTC, Alexander's stain, acetocarmine, iodine-potassium iodide and Sudan IV. Pollen viability estimated with TTC was 67.5% and similar to germination results providing a reliable estimate of in vitro germination. The four other stains overestimated pollen viability (> 90%), and their use should be restricted to determining cell constituents, and pollen grain integrity.

  16. Posfolklore: Raíces y globalización en la música popular chilena

    Directory of Open Access Journals (Sweden)

    González, Juan Pablo

    2011-10-01

    Full Text Available This article discusses the development of the concept of folk roots, introduced by Chilean singer-songwriters in the mid-seventies, as a strategy to legitimize their divergent practice from the norm of folklore in times of military rule. The institutionalization of this concept with the return of democracy in the early nineties and its continued tightening from a third generation of Chilean singersongwriters and bands derivatives from theater companies, allow us to discuss the problem of folk roots in times of globalization. We propose the concepts of posfolklore and hydroponic roots, attached to the trend of Chilean listeners and musicians to incorporate world music to their artistic practices and consumption. These concepts would facilitate the discussion of the mixing of influences in Chilean music and the consequent proliferation of intermediate rather than hegemonic identities in contemporary Chilean culture.

    Este artículo aborda el desarrollo del concepto de raíz folklórica, introducido por cantautores chilenos a mediados de los años setenta, como estrategia para legitimar corrientes divergentes del folklore de la norma imperante en tiempos del régimen militar. La institucionalización de este concepto con el retorno a la democracia y su continuo tensionamiento desde una tercera generación de cantautores nacionales y desde los nuevos grupos musicales derivados de compañías teatrales, permite reflexionar sobre el problema de las raíces en tiempos de globalización. Se proponen los conceptos de posfolklore y de raíces hidropónicas, adscritos a la permanente tendencia del músico y del auditor chileno a incorporar músicas del mundo a su práctica y consumo musical. Estos conceptos permiten discutir las mezclas en la música chilena y la consiguiente multiplicación de identidades intermedias más que hegemónicas en la cultura chilena contemporánea.

  17. Degradación cinética de carotenoides obtenidos de frutos de Carica papaya L Kinetic degradation of carotenoids from Carica papaya L. fruits

    OpenAIRE

    M.J. Moreno Alvarez; D. R. Belén Camacho; V Torrez

    2003-01-01

    Se evaluó la degradación de carotenoides extraídos de frutos de lechosa (Carica papaya L.). Los pigmentos previamente separados y liofilizados fueron almacenados en la oscuridad. Se midió su absorbancia a 440 nm, cada 15 días. Se determinó el orden de reacción, tiempo de vida media (t1/2) y constante de velocidad de degradación (k). La degradación de los carotenoides siguio una cinética de primer orden. El producto liofilizado presento t1/2: 43,3 días y k: 0,0160 días-1. Bajo estas condicione...

  18. Cyanogenic allosides and glucosides from Passiflora edulis and Carica papaya.

    Science.gov (United States)

    Seigler, David S; Pauli, Guido F; Nahrstedt, Adolf; Leen, Rosemary

    2002-08-01

    Leaf and stem material of Passiflora edulis (Passifloraceae) contains the new cyanogenic glycosides (2R)-beta-D-allopyranosyloxy-2-phenylacetonitrile (1a) and (2S)-beta-D-allopyranosyloxy-2-phenylacetonitrile (1b), along with smaller amounts of (2R)-prunasin (2a), sambunigrin (2b), and the alloside of benzyl alcohol (4); the major cyanogens of the fruits are (2R)-prunasin (2a) and (2S)-sambunigrin (2b). The major cyanogenic glycoside of Carica papaya (Caricaceae) is 2a; only small amounts of 2b also are present. We were not able to confirm the presence of a cyclopentenoid cyanogenic glycoside, tetraphyllin B, in Carica papaya leaf and stem materials. In detailed 1H NMR studies of 1a/b and 2a/b, differences in higher order effects in glucosides and allosides proved to be valuable for assignment of structures in this series. The diagnostic chemical shifts of cyanogenic methine and anomeric protons in 1a/b are sensitive to anisotropic environmental effects. The assignment of C-2 stereochemistry of 1a/b was made in analogy to previous assignments in the glucoside series and was supported by GLC analysis of the TMS ethers. PMID:12150815

  19. Papaya latex enzymes capable of detoxification of gliadin.

    Science.gov (United States)

    Cornell, H J; Doherty, W; Stelmasiak, T

    2010-01-01

    Assay of fractions obtained from ion exchange chromatography of papaya latex on CM Sephadex-C50, size exclusion chromatography on Sephacryl S-300 and size exclusion HPLC have provided an insight into the relative contributions of the gluten-detoxifying enzymes present. This outcome has been achieved by the use of the above chromatographic techniques, coupled with assays of lysosomal activity, protease activity using benzylarginine ethyl ester (BAEE) as substrate, prolyl endopeptidase (PEP) using glycylprolylnitroanilide and a prolidase assay using acetylprolylglycine. These procedures have shown that the activity in papaya latex is due largely to caricain and to a lesser extent, chymopapain and glutamine cyclotransferase. The presence of caricain and these other enzymes was confirmed by mass spectrometry of trypsin digests of the most active fraction obtained by CM Sephadex-C50 chromatography and size exclusion HPLC. Fractions rich in caricain would be suitable for enzyme therapy in gluten intolerance and appear to have synergistic action with porcine intestinal extracts. PMID:19156482

  20. Development of virus resistant transgenic papayas expressing the coat protein gene from a Brazilian isolate of Papaya ringspot virus Desenvolvimento de mamoeiros transgênicos resistentes a vírus expressando o gene da capa protéica de um isolado brasileiro de Papaya ringspot virus

    Directory of Open Access Journals (Sweden)

    Manoel T. Souza Júnior

    2005-08-01

    Full Text Available Translatable and nontranslatable versions of the coat protein (cp gene of a Papaya ringspot virus (PRSV isolate collected in the state of Bahia, Brazil, were engineered for expression in Sunrise and Sunset Solo varieties of papaya (Carica papaya. The biolistic system was used to transform secondary somatic embryo cultures derived from immature zygotic embryos. Fifty-four transgenic lines, 26 translatable and 28 nontranslatable gene versions, were regenerated, with a transformation efficiency of 2.7%. Inoculation of cloned R0 plants with PRSV BR, PRSV HA or PRSV TH, Brazilian, Hawaiian and Thai isolates, respectively, revealed lines with mono-, double-, and triple-resistance. After molecular analysis and a preliminary agronomic evaluation, 13 R1 and R2 populations were incorporated into the papaya-breeding program at Embrapa Cassava and Tropical Fruits, in Cruz das Almas, Bahia, Brazil.Versões traduzíveis e não traduzíveis do gene da capa protéica (cp de um isolado de Papaya ringspot virus (PRSV coletado no Estado da Bahia, Brasil, foram produzidas para expressão nas variedades Sunrise e Sunset Solo de mamoeiro (Carica papaya. O sistema de biobalística foi utilizado para transformar embriões somáticos secundários derivados de embriões zigóticos imaturos. Cinqüenta e quatro linhas transgênicas, sendo 26 contendo versões traduzíveis e 28 contendo versões não traduzíveis do gene cp foram regeneradas, o que resultou em 2,7% de eficiência de transformação, quando considerado o número de linhas transgênicas obtidas por embrião zigótico imaturo excisado. Desafios de plantas R0 com PRSV BR, PRSV HA ou PRSV TH, respectivamente isolado brasileiro, havaiano e tailandês, revelaram linhas com resistência a um, dois e três isolados de PRSV. Após análises moleculares e avaliação agronômica preliminar, 13 populações R1 e R2 de mamoeiros transgênicos foram incorporadas ao programa de melhoramento genético da Embrapa Mandioca e Fruticultura, em Cruz das Almas, Bahia, Brasil.

  1. Development of virus resistant transgenic papayas expressing the coat protein gene from a Brazilian isolate of Papaya ringspot virus / Desenvolvimento de mamoeiros transgênicos resistentes a vírus expressando o gene da capa protéica de um isolado brasileiro de Papaya ringspot virus

    Scientific Electronic Library Online (English)

    Manoel T., Souza Júnior; Osmar, Nickel; Dennis, Gonsalves.

    2005-08-01

    Full Text Available Versões traduzíveis e não traduzíveis do gene da capa protéica (cp) de um isolado de Papaya ringspot virus (PRSV) coletado no Estado da Bahia, Brasil, foram produzidas para expressão nas variedades Sunrise e Sunset Solo de mamoeiro (Carica papaya). O sistema de biobalística foi utilizado para transf [...] ormar embriões somáticos secundários derivados de embriões zigóticos imaturos. Cinqüenta e quatro linhas transgênicas, sendo 26 contendo versões traduzíveis e 28 contendo versões não traduzíveis do gene cp foram regeneradas, o que resultou em 2,7% de eficiência de transformação, quando considerado o número de linhas transgênicas obtidas por embrião zigótico imaturo excisado. Desafios de plantas R0 com PRSV BR, PRSV HA ou PRSV TH, respectivamente isolado brasileiro, havaiano e tailandês, revelaram linhas com resistência a um, dois e três isolados de PRSV. Após análises moleculares e avaliação agronômica preliminar, 13 populações R1 e R2 de mamoeiros transgênicos foram incorporadas ao programa de melhoramento genético da Embrapa Mandioca e Fruticultura, em Cruz das Almas, Bahia, Brasil. Abstract in english Translatable and nontranslatable versions of the coat protein (cp) gene of a Papaya ringspot virus (PRSV) isolate collected in the state of Bahia, Brazil, were engineered for expression in Sunrise and Sunset Solo varieties of papaya (Carica papaya). The biolistic system was used to transform seconda [...] ry somatic embryo cultures derived from immature zygotic embryos. Fifty-four transgenic lines, 26 translatable and 28 nontranslatable gene versions, were regenerated, with a transformation efficiency of 2.7%. Inoculation of cloned R0 plants with PRSV BR, PRSV HA or PRSV TH, Brazilian, Hawaiian and Thai isolates, respectively, revealed lines with mono-, double-, and triple-resistance. After molecular analysis and a preliminary agronomic evaluation, 13 R1 and R2 populations were incorporated into the papaya-breeding program at Embrapa Cassava and Tropical Fruits, in Cruz das Almas, Bahia, Brazil.

  2. Efficacy of Essential Oils on the Conidial Germination, Growth of Colletotrichum gloeosporioides (Penz. Penz. and Sacc and Control of Postharvest Diseases in Papaya (Carica papaya L.

    Directory of Open Access Journals (Sweden)

    Laura Leticia Barrera-Necha

    2008-01-01

    Full Text Available The efficacy of nine essential oils were investigated in vitro on conidial germination and mycelial growth inhibition of Colletotrichum gloeosporioides isolate from papaya (Carica papaya L.. In general, better antifungal effect was observed with Cinnamomum zeylanicum and Syzygium aromaticum oils which had strong inhibition of conidial germination of C. gloeosporioides at 50, 100, 150, 200 and 250 ?g mL-1 and a dose dependent inhibition mycelial growth was caused by these oils. Teloxys ambrosioides, Mentha piperita and Ruta chalepensis oils exhibited a moderate action at 150, 200 and 250 ?g mL-1 on conidial germination and mycelial growth inhibition. Allium sativum, Citrus aurantifolia and Eucalyptus globulus oils had no antifungal activity at different concentration. Taking into account the in vitro results, C. zeylanicum and S. aromaticum oils were evaluated on papaya fruit during storage at ambient temperature and 14°C. The lowest infection percentage were for papaya fruits treated with S. aromaticum at 50 ?g mL-1 at both temperature tested, nevertheless did not overcome the activity of synthetic fungicide. After storage at both temperature, values of Soluble Solids Content (SSC was not significantly different. S. aromaticum oils may be a possibility to control C. gloeosporioides of papaya fruit.

  3. Immobilization of mycoplana sp. MVMB2 isolated from petroleum contaminated soil onto papaya stem (carica papaya l.) and its application on degradation of phenanthrene

    International Nuclear Information System (INIS)

    This study presents the degradation of phenanthrene using immobilized Mycoplana sp. MVMB2 isolated from contaminated soil. Papaya stem pretreated by two stage processes, treating with acid or alkali and drying, was used for the immobilization of Mycoplana sp. Alkali pretreated papaya stem was found to be most effective in cell uptake compared to acid treated one. The maximum immobilization capacity at various physiochemical conditions for the alkali pretreated papaya stem was found to be at 320 min time, pH 6.5, 30 C temperature, and 18.6 x 106 cells/mL initial concentrations. The adsorption mechanism of Mycoplana sp. MVMB2 on pretreated papaya stem was assessed using various kinetic and isotherm models. The immobilization of Mycoplana sp. MVMB2 on to pretreated papaya stem was corroborated by scanning electron microscopy and Fourier transformed IR spectroscopy analysis. The performance of immobilized cells in batch reactor showed more than 95% phenanthrene degradation within 72 h, whereas, free cells were found to require 120 h. The immobilized cells also showed better degradation performance in the packed column study. (Copyright copyright 2012 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  4. Influence of gamma radiation on carbohydrates metabolism of ripening papaya (Carica papaya L. cv. Solo); Metabolismo de carboidratos durante o amadurecimento do mamao (Carica papaya L. Cv. Solo): influencia da radiacao gama

    Energy Technology Data Exchange (ETDEWEB)

    Gomez, M.L.P.A. [Universidade de Sao Paulo (FCF/USP), SP (Brazil). Fac. de Ciencias Farmaceuticas; Lajolo, F.M.; Cordenunsi, B.R. [Universidade de Sao Paulo (FCF/USP), SP (Brazil). Fac. de Ciencias Farmaceuticas. Dept. de Alimentos e Nutricao Experimental

    1999-05-15

    Food irradiation is one of the most promising treatments that can be utilized for fruits disinfestation and extension of shelf life. The authors studied the influence of 0,5 kGy of Gamma irradiation on the soluble carbohydrates composition of papaya (Carica papaya L. cv. Solo) fruit, and on sucrose metabolizing enzymes: sucrose synthase (SS), sucrose-phosphate synthase, acid and neutral invertases activities, during ripening. The results demonstrated that ethylene production, total soluble sugars, sucrose content, and sucrose-phosphate synthase and invertases activities were affected by irradiation, but not respiration, glucose and fructose content, and SS activity. (author)

  5. Screening of papaya accessions resistant to Papaya lethal yellowing virus and capacity of Tetranychus urticae to transmit the virus / Identificação de acessos de mamoeiro resistentes ao Papaya lethal yellowing virus e capacidade de Tetranychus urticae em transmitir o vírus

    Scientific Electronic Library Online (English)

    Marcos Fernando, Basso; Álvaro Júlio, Pereira; Hermano Monteiro de Barros, Pereira; Humberto Josué de Oliveira, Ramos; Jorge Luiz Loyola, Dantas; Elizabeth Pacheco Batista, Fontes; Eduardo Chumbinho de, Andrade; Francisco Murilo, Zerbini.

    2015-02-01

    Full Text Available O objetivo deste trabalho foi produzir um antissoro policlonal contra a proteína capsidial (PC) do Papaya lethal yellowing virus (PLYV) e determinar sua especificidade e sensibilidade na diagnose do vírus, bem como avaliar a resistência genética de acessos de mamoeiro (Carica papaya) ao PLYV e inves [...] tigar a capacidade do ácaro rajado Tetranychus urticae em adquirir e transmitir o vírus às plantas. Foram avaliados 65 acessos de mamoeiro. Para cada acesso, dez plantas foram submetidas à inoculação mecânica com extratos de plantas infectadas com PLYV, e três plantas receberam inoculação apenas com tampão de fosfato e foram usadas como controle negativo. Noventa dias após a inoculação, novas folhas sistêmicas emergentes foram coletadas das plantas inoculadas, e a infecção viral foi diagnosticada por Elisa indireto, com uso de antissoro policlonal sensível à PC do PLYV expressa in vitro. A transmissão viral por T. urticae foi avaliada em casa de vegetação. Os experimentos foram repetidos duas vezes. O antissoro policlonal reconheceu a PC do PLYV especificamente e discriminou a infecção pelo PLYV de infecções causadas por outros vírus. Dos 65 acessos de mamoeiros avaliados, 15 foram considerados resistentes, 18 moderadamente resistentes e 32 suscetíveis. O ácaro rajado T. urticae foi capaz de adquirir o PLYV, mas não de transmiti-lo para o mamoeiro. Abstract in english The objective of this work was to produce a polyclonal antiserum against the coat protein (CP) of Papaya lethal yellowing virus (PLYV) and to determine its specificity and sensibility in the diagnosis of the virus, as well as to evaluate the genetic resistance to PLYV in papaya (Carica papaya) acces [...] sions and to investigate the capacity of the two-spotted spider mite Tetranychus urticae to acquire and transmit PLYV to the plants. Sixty-five papaya accessions were evaluated. For each accession, ten plants were mechanically inoculated using PLYV-infected plant extracts, and three plants were mock inoculated with phosphate buffer alone and used as negative controls. Ninety days after inoculation, newly-emerging systemic leaves were collected from the inoculated plants, and viral infection was diagnosed by indirect Elisa, using polyclonal antiserum sensible to the in vitro-expressed PLYV CP. Viral transmission by T. urticae was evaluated in greenhouse. The experiments were repeated twice. Polyclonal antiserum recognized the recombinant PLYV CP specifically and discriminated PLYV infection from infections caused by other plant viruses. Out of the 65 papaya accessions evaluated, 15 were considered resistant, 18 moderately resistant, and 32 susceptible. The two-spotted spider mite T. urticae was capable of acquiring PLYV, but not of transmitting it to papaya.

  6. In vitro erythrocyte membrane stabilization properties of Carica papaya L. leaf extracts

    Directory of Open Access Journals (Sweden)

    Priyanga Ranasinghe

    2012-01-01

    Full Text Available Background : Carica papaya L. fruit juice and leaf extracts are known to have many beneficial medical properties. Recent reports have claimed possible beneficial effects of C. papaya L. leaf juice in treating patients with dengue viral infections. This study aims to evaluate the membrane stabilization potential of C. papaya L. leaf extracts using an in vitro hemolytic assay. Materials and Methods: The study was conducted in between June and August 2010. Two milliliters of blood from healthy volunteers and patients with serologically confirmed current dengue infection were freshly collected and used in the assays. Fresh papaya leaves at three different maturity stages (immature, partly matured, and matured were cleaned with distilled water, crushed, and the juice was extracted with 10 ml of cold distilled water. Freshly prepared cold water extracts of papaya leaves (1 ml containing 30 μl of papaya leaf extracts, 20 μl from 40% erythrocytes suspension, and 950 μl of phosphate buffered saline were used in the heat-induced and hypotonic-induced hemolytic assays. In dose response experiments, six different concentrations (9.375, 18.75, 37.5, 75, 150, and 300 μg/ml of freeze dried extracts of the partly matured leaves were used. Membrane stabilization properties were investigated with heat-induced and hypotonicity-induced hemolysis assays. Results: Extracts of papaya leaves of all three maturity levels showed a significant reduction in heat-induced hemolysis compared to controls (P 0.05 different from one another. Heat-induced hemolysis inhibition activity did not demonstrate a linear dose response relationship. At 37.5 μg/ml concentration of the extract, a marked inhibition of hypotonicity-induced hemolysis was observed. Conclusion: C. papaya L. leaf extracts showed a significant inhibition of hemolysis in vitro and could have a potential therapeutic effect on disease processes causing destabilization of biological membranes.

  7. Carica papaya induces in vitro thrombopoietic cytokines secretion by mesenchymal stem cells and haematopoietic cells

    OpenAIRE

    Aziz, Jazli; Abu Kassim, Noor Lide; Abu Kasim, Noor Hayaty; Haque, Nazmul; Rahman, Mohammad Tariqur

    2015-01-01

    Background Use of Carica papaya leaf extracts, reported to improve thrombocyte counts in dengue patients, demands further analysis on the underlying mechanism of its thrombopoietic cytokines induction Methods In vitro cultures of peripheral blood leukocytes (PBL) and stem cells from human exfoliated deciduous teeth (SHED) were treated with unripe papaya pulp juice (UPJ) to evaluate its potential to induce thrombopoietic cytokines (IL-6 and SCF) Results In vitro scratch gap closure was signifi...

  8. Protective effect of dried fruits of Carica papaya on hepatotoxicity in rat

    OpenAIRE

    Md. Zafor Sadeque, Zinnat Ara Begum

    2010-01-01

    Aqueous and ethanol extracts of Carica papaya has been evaluated for its hepatoprotective activity in rat. The aqueous and ethanol extracts of Carica papaya showed significant hepatoprotection against carbon tetrachloride induced hepatotoxicity. The protective activity was evaluated by using biochemical parameters such as serum bilirubin, serum alanine amino transferase (ALT), aspartate amino transferase (AST) and alkaline phosphatase. The histopathological changes of liver were compared with...

  9. Hypoglycemic effect of Carica papaya leaves in streptozotocin-induced diabetic rats

    OpenAIRE

    Juárez-Rojop Isela Esther; Díaz-Zagoya Juan C; Ble-Castillo Jorge L; Miranda-Osorio Pedro H; Castell-Rodríguez Andrés E; Tovilla-Zárate Carlos A; Rodríguez-Hernández Arturo; Aguilar-Mariscal Hidemi; Ramón-Frías Teresa; Bermúdez-Ocaña Deysi Y

    2012-01-01

    Abstract Background Traditional plant treatment for diabetes has shown a surging interest in the last few decades. Therefore, the purpose of this study was to assess the hypoglycemic effect of the aqueous extract of C. papaya leaves in diabetic rats. Several studies have reported that some parts of the C. papaya plant exert hypoglycemic effects in both animals and humans. Methods Diabetes was induced in rats by intraperitoneal administration of 60 mg/kg of streptozotocin (STZ). The aqueous ex...

  10. Antifungal Activity in Ethanolic Extracts of Carica papaya L. cv. Maradol Leaves and Seeds

    OpenAIRE

    Chávez-Quintal, Pedro; González-Flores, Tania; Rodríguez-Buenfil, Ingrid; Gallegos-Tintoré, Santiago

    2011-01-01

    Bioactive compounds from vegetal sources are a potential source of natural antifungic. An ethanol extraction was used to obtain bioactive compounds from Carica papaya L. cv. Maradol leaves and seeds of discarded ripe and unripe fruit. Both, extraction time and the papaya tissue flour:organic solvent ratio significantly affected yield, with the longest time and highest flour:solvent ratio producing the highest yield. The effect of time on extraction efficiency was confirmed by qualitative iden...

  11. Isolation of natural inhibitors of papain obtained from Carica papaya latex

    OpenAIRE

    Rubens Monti; Jonas Contiero; Antonio José Goulart

    2004-01-01

    Studies were carried out to natural papain inhibitor from papaya latex. Fresh latex from green fruits of Carica papaya was collected and immediately transported in ice bath to the lab, from which three fractions with inhibitor effect of esterase papain activity were isolated by latex dialysis, Sephadex G-25 gel filtration and ionic exchange chromatography in SP-Sephadex C-25. The isolated fractions, identified as inhibitors I and II, showed a negative reaction with ninhydrin; however, the fra...

  12. Compositional difference in antioxidant and antibacterial activity of all parts of the Carica papaya using different solvents

    OpenAIRE

    Asghar, Nazia; Naqvi, Syed Ali Raza; Hussain, Zaib; Rasool, Nasir; Khan, Zulfiqar Ali; Shahzad, Sohail Anjum; Sherazi, Tauqir A.; Janjua, Muhammad Ramzan Saeed Ashraf; Nagra, Saeed Ahmad; Zia-Ul-Haq, Muhammad; Jaafar, Hawa ZE

    2016-01-01

    Background Carica papaya is a well known medicinal plant used in the West and Asian countries to cope several diseases. Patients were advised to eat papaya fruit frequently during dengue fever epidemic in Pakistan by physicians. This study was conducted to establish Polyphenols, flavonoids and antioxidant potential profile of extracts of all major parts of the C. papaya with seven major solvents i.e. water, ethanol, methanol, n-butanol, dichloromethane, ethyl acetate, and n-hexane. Results TP...

  13. Papain protects papaya trees from herbivorous insects: role of cysteine proteases in latex.

    Science.gov (United States)

    Konno, Kotaro; Hirayama, Chikara; Nakamura, Masatoshi; Tateishi, Ken; Tamura, Yasumori; Hattori, Makoto; Kohno, Katsuyuki

    2004-02-01

    Many plants contain latex that exudes when leaves are damaged, and a number of proteins and enzymes have been found in it. The roles of those latex proteins and enzymes are as yet poorly understood. We found that papain, a cysteine protease in latex of the Papaya tree (Carica papaya, Caricaceae), is a crucial factor in the defense of the papaya tree against lepidopteran larvae such as oligophagous Samia ricini (Saturniidae) and two notorious polyphagous pests, Mamestra brassicae (Noctuidae) and Spodoptera litura (Noctuidae). Leaves of a number of laticiferous plants, including papaya and a wild fig, Ficus virgata (Moraceae), showed strong toxicity and growth inhibition against lepidopteran larvae, though no apparent toxic factors from these species have been reported. When the latex was washed off, the leaves of these lactiferous plants lost toxicity. Latexes of both papaya and the wild fig were rich in cysteine-protease activity. E-64, a cysteine protease-specific inhibitor, completely deprived the leaves of toxicity when painted on the surface of papaya and fig leaves. Cysteine proteases, such as papain, ficin, and bromelain, all showed toxicity. The results suggest that plant latex and the proteins in it, cysteine proteases in particular, provide plants with a general defense mechanism against herbivorous insects. PMID:14731257

  14. Effects of Ripe Carica Papaya Seed Powder on Testicular Histology of Boars

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    Kipyegon AN*, HM Mutembei, VT Tsuma and JA Oduma1

    2012-09-01

    Full Text Available The effect of oral administration of ripe Carica Papaya seed powder on testicular histology of Sus scrofa domestica boars has been addressed in the present article. Fifteen pubertal Large White boars were randomly selected and divided into two groups. Each boar in the experimental group received a daily dose of 300mg C Papaya mixed with 0.5 kg of conventional pig feed while the control group received a placebo. The experiment was carried out for 56 days in the University of Nairobi. After every two weeks, one boar from the control group and two from the experimental group were castrated and testicular tissue samples processed for histology. At the end of 56 days the remaining entire boars were maintained for 14 days and 60 days respectively without the papaya powder to assess reversibility. The test material had no effect on haematological parameters. However, histopathological changes of the seminiferous epithelium which appeared to be dependent upon duration of C Papaya consumption was noticed, these changes were reversible.Although the mechanism(s for the effect of papaya seed extract is not explained by this study, it is observed that papaya seed powder causes gradual disorganization, exfoliation and loss of spermatocytes and spermatids.

  15. Oral dosing with papaya latex is an effective anthelmintic treatment for sheep infected with Haemonchus contortus

    Directory of Open Access Journals (Sweden)

    Donnan Alison A

    2011-03-01

    Full Text Available Abstract Background The cysteine proteinases in papaya latex have been shown to have potent anthelmintic properties in monogastric hosts such as rodents, pigs and humans, but this has not been demonstrated in ruminants. Methods In two experiments, sheep were infected concurrently with 5,000 infective larvae of Haemonchus contortus and 10,000 infective larvae of Trichostrongylus colubriformis and were then treated with the supernatant from a suspension of papaya latex from day 28 to day 32 post-infection. Faecal egg counts were monitored from a week before treatment until the end of the experiment and worm burdens were assessed on day 35 post-infection. Results We found that the soluble fraction of papaya latex had a potent in vivo effect on the abomasal nematode H. contortus, but not on the small intestinal nematode T. colubriformis. This effect was dose-dependent and at tolerated levels of gavage with papaya latex (117 ?mol of active papaya latex supernatant for 4 days, the H. contortus worm burdens were reduced by 98%. Repeated treatment, daily for 4 days, was more effective than a single dose, but efficacy was not enhanced by concurrent treatment with the antacid cimetidine. Conclusions Our results provide support for the idea that cysteine proteinases derived from papaya latex may be developed into novel anthelmintics for the treatment of lumenal stages of gastro-intestinal nematode infections in sheep, particularly those parasitizing the abomasum.

  16. La familia Hemiaulaceae (Bacillariophyceae) de las aguas marinas chilenas The family Hemiaulaceae (Bacillariophyceae) from marine Chilean waters

    OpenAIRE

    PATRICIO RIVERA; SERGIO AVARIA; FABIOLA CRUCES

    2003-01-01

    La familia Hemiaulaceae comprende cuatro géneros, señalados previamente para las aguas chilenas con las siguientes especies: Cerataulina pelagica (Cleve) Hendey, Hemiaulus sinensis Greville, H. membranaceus Cleve, Climacodium biconcavum Cleve, Eucampia antarctica (Castracane) Mangin, E. cornuta (Cleve) Grunow y E. zodiacus Ehrenberg. Sin embargo, el análisis con microscopía fotónica y electrónica de muestras recolectadas en aguas marinas chilenas que contenían representantes de esta familia (...

  17. Gamma radiation use as a quarantine treatment for Ceratitis capitata (Wied., 1824) and Anastrepha fraterculus (Wied., 1830) in papaya fruits (Carica papaya, Linnaeus), cultivar sunrise solo

    International Nuclear Information System (INIS)

    The gamma irradiation as treatment for control of the immature stages of fruit flies, C. capitata and A. fraterculus in papaya fruits, cultivar Sunrise Solo is studied. The gamma rays attenuation was observed by one and two papaya fruits placed between source and infested fruit, and this changed the radiation doses required to obtain mortality in eggs and larvae of the Medfly. The efficacy of irradiation was higher when the infested papayas were packed in paper box. The radiation dose determined using the Probit 9 concept, for immature stages mortality of C. capitata was 107,74 Gy and for A. fraterculus was 50,82 Gy. The sterilizing doses for adults of C. capitata and D. fraterculus, irradiated as pupae, was 60 Gy and 40 Gy, respectively. (author)

  18. Genótipos melhorados de mamão (Carica papaya L.): avaliação tecnológica dos frutos na forma de sorvete / Improved genotypes of papaya (Carica papaya L.): technological evaluation as ice-cream

    Scientific Electronic Library Online (English)

    Ligia R.R., Santana; Fernando C.A.U., Matsuura; Ricardo L., Cardoso.

    2003-12-01

    Full Text Available O presente trabalho teve como objetivo a avaliação tecnológica na forma de sorvete, de frutos de cinco genótipos selecionados de mamão (CMF012, CMF020, CMF023, CMF031, CMF047), provenientes do Banco Ativo de Germoplasma da Embrapa Mandioca e Fruticultura, Cruz das Almas, Bahia. Estes genótipos foram [...] identificados em estudos anteriores por apresentarem cor atraente, sabor agradável e valores elevados de sólidos solúveis (ºBrix). As amostras de sorvete foram submetidas à avaliação sensorial para os atributos aparência, cor, aroma, sabor e textura, através de testes afetivos, utilizando-se escala hedônica estruturada de nove pontos. Realizou-se determinações de pH, acidez total titulável, sólidos solúveis (ºBrix), "ratio", ácido ascórbico, sólidos totais, cinzas, proteínas, lipídeos, açúcares totais, redutores e não-redutores. Os resultados das avaliações sensoriais e físico-químicas foram analisados através de ANOVA e teste de Tukey. Os sorvetes demonstraram elevada aceitação, obtendo médias correspondentes ao conceito "gostei moderadamente" para todos os atributos avaliados. Os genótipos CMF020 e CMF031 destacaram-se alcançando média correspondente ao termo da escala hedônica "gostei muito" para o atributo sabor. Os provadores não indicaram prevalência entre os produtos quanto à aparência, cor e textura; portanto, os genótipos pouco atraentes como fruto de mesa, poderão ser aproveitados na indústria para elaboração de sorvete. Além disso, podem ser considerados como uma sobremesa valiosa e nutritiva, pois apresentaram níveis satisfatórios de carboidratos e razoáveis de vitamina C e proteínas. O estudo demonstrou que o sorvete de mamão é uma excelente alternativa para o aproveitamento da fruta. Abstract in english The objective of the present work was to evaluate as an alternative of consumption the ice-cream of five selective papaya genotypes (CMF012, CMF020, CMF023, CMF031, CMF047) obtained from the Active Germplasm Bank for Papaya at Embrapa Cassava and Fruit Crops, located in Cruz das Almas, Bahia State, [...] Brazil. These genotypes were identified at before studies because they showed atractive colour, good flavour and highter soluble solid (ºBrix). Sensory analyses of the ice-creams were carried out using affective tests (9 point hedonic scale) for the attributes of overall appearance, colour, odour, flavour and texture. The following physico-chemical determinations were carried out: pH, total titrable acidity, soluble solid (ºBrix), ratio, vitamin C, total solid, ash, protein, lipid, total sugars, reducing and non-reducing sugars.The sensory and physico-chemical data were submitted to an analysis of variation, followed the Tukey test. The ice creams showed higher scores for all of attributes. The genotypes CMF020 and CMF031 were preferred in terms of flavour. The judges didn!t indicate preference among the products in terms of overall appearance, colour and texture, so the less atractive genotypes for the fresh fruit market, could be utilized for the industry to make ice cream. They are a nutritive dessert, because they showed enough carbohydrates, vitamin C and protein contents. This study showed that the papaya ice cream is an excellent alternative of consumption.

  19. Genótipos melhorados de mamão (Carica papaya L.: avaliação tecnológica dos frutos na forma de sorvete Improved genotypes of papaya (Carica papaya L.: technological evaluation as ice-cream

    Directory of Open Access Journals (Sweden)

    Ligia R.R. Santana

    2003-12-01

    Full Text Available O presente trabalho teve como objetivo a avaliação tecnológica na forma de sorvete, de frutos de cinco genótipos selecionados de mamão (CMF012, CMF020, CMF023, CMF031, CMF047, provenientes do Banco Ativo de Germoplasma da Embrapa Mandioca e Fruticultura, Cruz das Almas, Bahia. Estes genótipos foram identificados em estudos anteriores por apresentarem cor atraente, sabor agradável e valores elevados de sólidos solúveis (ºBrix. As amostras de sorvete foram submetidas à avaliação sensorial para os atributos aparência, cor, aroma, sabor e textura, através de testes afetivos, utilizando-se escala hedônica estruturada de nove pontos. Realizou-se determinações de pH, acidez total titulável, sólidos solúveis (ºBrix, "ratio", ácido ascórbico, sólidos totais, cinzas, proteínas, lipídeos, açúcares totais, redutores e não-redutores. Os resultados das avaliações sensoriais e físico-químicas foram analisados através de ANOVA e teste de Tukey. Os sorvetes demonstraram elevada aceitação, obtendo médias correspondentes ao conceito "gostei moderadamente" para todos os atributos avaliados. Os genótipos CMF020 e CMF031 destacaram-se alcançando média correspondente ao termo da escala hedônica "gostei muito" para o atributo sabor. Os provadores não indicaram prevalência entre os produtos quanto à aparência, cor e textura; portanto, os genótipos pouco atraentes como fruto de mesa, poderão ser aproveitados na indústria para elaboração de sorvete. Além disso, podem ser considerados como uma sobremesa valiosa e nutritiva, pois apresentaram níveis satisfatórios de carboidratos e razoáveis de vitamina C e proteínas. O estudo demonstrou que o sorvete de mamão é uma excelente alternativa para o aproveitamento da fruta.The objective of the present work was to evaluate as an alternative of consumption the ice-cream of five selective papaya genotypes (CMF012, CMF020, CMF023, CMF031, CMF047 obtained from the Active Germplasm Bank for Papaya at Embrapa Cassava and Fruit Crops, located in Cruz das Almas, Bahia State, Brazil. These genotypes were identified at before studies because they showed atractive colour, good flavour and highter soluble solid (ºBrix. Sensory analyses of the ice-creams were carried out using affective tests (9 point hedonic scale for the attributes of overall appearance, colour, odour, flavour and texture. The following physico-chemical determinations were carried out: pH, total titrable acidity, soluble solid (ºBrix, ratio, vitamin C, total solid, ash, protein, lipid, total sugars, reducing and non-reducing sugars.The sensory and physico-chemical data were submitted to an analysis of variation, followed the Tukey test. The ice creams showed higher scores for all of attributes. The genotypes CMF020 and CMF031 were preferred in terms of flavour. The judges didn!t indicate preference among the products in terms of overall appearance, colour and texture, so the less atractive genotypes for the fresh fruit market, could be utilized for the industry to make ice cream. They are a nutritive dessert, because they showed enough carbohydrates, vitamin C and protein contents. This study showed that the papaya ice cream is an excellent alternative of consumption.

  20. Época de colheita dos frutos e ocorrência de dormência em sementes de mamão (Carica papaya L. Fruit harvest season and dormancy occurrency in papaya seeds (Carica papaya L.

    Directory of Open Access Journals (Sweden)

    Daí Tokuhisa

    2008-01-01

    Full Text Available O trabalho teve como objetivo relacionar a época de colheita dos frutos com a ocorrência de dormência nas sementes de mamão. Foram utilizados frutos de mamão do grupo Formosa, híbrido Tainung 01, colhidos no estádio 1 de maturação (até 15% da superfície externa com coloração amarela em cinco diferentes épocas do ano: Julho e Novembro/2004 e Fevereiro, Maio e Setembro/2005. Os frutos permaneceram armazenados em ambiente de laboratório até atingirem o estádio 5 de maturação (mais de 75% da superfície externa com coloração amarela. Em cada época, foram obtidas sementes com e sem sarcotesta, que foram submetidas ao teste de germinação, conduzido em germinador sob temperatura alternada de 20-30°C (16h/8h, respectivamente, avaliando-se a porcentagem de plântulas normais aos 15 e 30 dias após a semeadura. Verificou-se que a presença da sarcotesta na semente diminui a velocidade e a porcentagem de germinação. Houve efeito da época de colheita do fruto na germinação das sementes, sendo que as sementes extraídas de frutos colhidos em Julho/2004 e Maio/2005 apresentaram dormência pós-colheita, o que não ocorreu com as sementes obtidas de frutos que amadureceram em épocas de temperaturas mais altas.This study was carried out in order to relate the fruit harvest season and dormancy occurrence in papaya seeds. Papaya fruits of the Formosa group, Tainung 01 hybrid, were harvested at maturation stage 1 (15% external color yellow, at five different times: July and November/2004 and February, May and September/2005. The fruits were stored at room temperature until they reached maturation stage 5 (more than 75% external color yellow. Seeds with and without sarcotesta were obtained at each harvest time. Standard germination of these seeds was performed in a germinator at 20/30 º C (8/16 hours, respectively and normal seedlings percentage was evaluated at 15 and 30 days. Seeds with sarcotesta had lower germination speed and percentage. There was an effect of fruit harvest time on seed germination. Seeds extracted from fruits harvested in July/2004 and May/2005 had post-harvest dormancy, which did not occur in seeds from fruits harvested in the high temperature periods.

  1. Época de colheita dos frutos e ocorrência de dormência em sementes de mamão (Carica papaya L.) / Fruit harvest season and dormancy occurrency in papaya seeds (Carica papaya L.)

    Scientific Electronic Library Online (English)

    Daí, Tokuhisa; Denise Cunha Fernandes dos Santos, Dias; Eveline Mantovani, Alvarenga; Luiz Antônio dos, Santos Dias; Sérgio Lúcio David, Marin.

    Full Text Available O trabalho teve como objetivo relacionar a época de colheita dos frutos com a ocorrência de dormência nas sementes de mamão. Foram utilizados frutos de mamão do grupo Formosa, híbrido Tainung 01, colhidos no estádio 1 de maturação (até 15% da superfície externa com coloração amarela) em cinco difere [...] ntes épocas do ano: Julho e Novembro/2004 e Fevereiro, Maio e Setembro/2005. Os frutos permaneceram armazenados em ambiente de laboratório até atingirem o estádio 5 de maturação (mais de 75% da superfície externa com coloração amarela). Em cada época, foram obtidas sementes com e sem sarcotesta, que foram submetidas ao teste de germinação, conduzido em germinador sob temperatura alternada de 20-30°C (16h/8h, respectivamente), avaliando-se a porcentagem de plântulas normais aos 15 e 30 dias após a semeadura. Verificou-se que a presença da sarcotesta na semente diminui a velocidade e a porcentagem de germinação. Houve efeito da época de colheita do fruto na germinação das sementes, sendo que as sementes extraídas de frutos colhidos em Julho/2004 e Maio/2005 apresentaram dormência pós-colheita, o que não ocorreu com as sementes obtidas de frutos que amadureceram em épocas de temperaturas mais altas. Abstract in english This study was carried out in order to relate the fruit harvest season and dormancy occurrence in papaya seeds. Papaya fruits of the Formosa group, Tainung 01 hybrid, were harvested at maturation stage 1 (15% external color yellow), at five different times: July and November/2004 and February, May a [...] nd September/2005. The fruits were stored at room temperature until they reached maturation stage 5 (more than 75% external color yellow). Seeds with and without sarcotesta were obtained at each harvest time. Standard germination of these seeds was performed in a germinator at 20/30 º C (8/16 hours, respectively) and normal seedlings percentage was evaluated at 15 and 30 days. Seeds with sarcotesta had lower germination speed and percentage. There was an effect of fruit harvest time on seed germination. Seeds extracted from fruits harvested in July/2004 and May/2005 had post-harvest dormancy, which did not occur in seeds from fruits harvested in the high temperature periods.

  2. Heat treatment and gamma radiation on the control of Colletotrichum gloeosporioides (Penz.) Penz. et Sacc., causal agent of the papaya fruits (Carica papaya L.) anthracnose

    International Nuclear Information System (INIS)

    For controlling papaya anthracnose on stored fruits the effect of heat treatment and gamma radiation on Colletotrichum gloeosporioides was studied in vitro and in vivo. Some physiological studies with four isolades of C. gloeosporioides from papaya fruits concerning the best conditions for mycelial growth and sporulation were also performed. The results showed that the best conditions for isolates development in BDA were: temperature between 25 and 270 C for mycelium radial growth; between 27 and 300 C under 12 hours alternating periods of fluorescent). (author). 131 refs., 9 figs., 33 tabs

  3. Morfología de la flor y de la semilla de papaya (carica papaya l.): variedad maradol e híbrido tainung-1

    OpenAIRE

    Gil, Arlette Ivonne; Miranda, Diego

    2010-01-01

    Con el objetivo de analizar la morfología de la flor y de la semilla de papaya (Carica papaya L.) de la variedad ‘Maradol’ y el híbrido ‘Tainung-1’, se recolectó el material vegetal en dos plantaciones y se llevaron al laboratorio de Fisiología de Cultivos, Facultad de Agronomía, Universidad Nacional de Colombia, Bogotá, con el fin de realizar las descripciones correspondientes de los tres tipos de flores (femeninas, hermafroditas y estaminadas) y de la semilla, sus características externas (...

  4. Nematodos fitoparásitos y su relación con factores edáficos de papaya en Colima, México / Plant parasitic nematodes and its relation to soil factors of papaya in Colima, Mexico

    Scientific Electronic Library Online (English)

    José Ángel, Martínez Gallardo; Tomás, Díaz Valdés; Leopoldo, Partida Ruvalcaba; Raúl, Allende Molar; José Benigno, Valdez Torres; José Armando, Carrillo Fasio.

    2015-02-01

    Full Text Available Dentro de este trabajo, en un artículo previo se presentó la identificación y cuantificación de las poblaciones de nematodos fitoparásitos presentes y asociados en cultivos de papaya (Carica papaya L.) en Colima, México. El objetivo del presente trabajo fue determinar la relación entre factores edáf [...] icos (textura, contenido de materia orgánica, conductividad eléctrica y pH) y poblaciones de nematodos fitoparásitos presentes en el cultivo de papaya en Colima, México. Se colectaron muestras de raíz y suelo rizosférico en 10 predios de siete ranchos en los municipios de Colima y Tecomán, Colima, México cultivados con papaya ‘Maradol’, ‘Tainung’ y ‘Sensation’. Del suelo y raíz colectados se detectaron e identificaron las poblaciones de nematodos; asimismo, se determinaron las características edáficas en cada sitio de muestreo. Se realizó una prueba ji-cuadrada de Pearson para probar la asociación entre los géneros de fitonematodos y los distintos tipos de texturas edáficas. Así mismo, se realizó un análisis de regresión lineal múltiple entre la población de nematodos y factores edáficos. Las texturas de suelo identificadas fueron arenoso franca, franco arenosa, franco arcillo arenosa y arenosa. Existió correlación entre los géneros de fitonematodos y los tipos de textura del suelo, encontrándose a la textura arenosa franca como la más apta para el ciclo de vida de los nematodos fitoparásitos. Abstract in english Within this article, a previous article presented the identification and quantification of populations of plant parasitic nematodes associated with papaya (Carica papaya L.) in Colima, Mexico. The aim of this study was to determine the relationship between soil factors (texture, organic matter conte [...] nt, electrical conductivity and pH) and populations of plant parasitic nematodes in papaya in Colima, Mexico. Root samples and soil from the rhizospherere were taken in 10 pieces of land in seven ranches from the municipalities of Colima and Tecomán, in Colima, Mexico; cultivated with papaya 'Maradol', 'Tainung' and 'Sensation'. From soil and roots collected, were detected and identified nematode populations; also soil characteristics were determined at each sampling site. A chi-square distribution or Pearson test was performed, to test the association between plant nematodes genus and different types of soil textures. Likewise, a multiple linear regression between nematode populations and soil factors was performed. Soil textures were loamy sand, sandy loam, sandy clay loam and sandy clay. There is a correlation between phytonematodes genus and types of soil texture, finding sandy loam texture as most suitable for the life cycle of plant parasitic nematodes.

  5. EVALUACIÓN DE DOS HÍBRIDOS DE PAPAYA INTRODUCIDOS EN CUBA

    Directory of Open Access Journals (Sweden)

    Maruchi Alonso

    2009-01-01

    Full Text Available En el presente trabajo se realizó la caracterización morfológica de 2 híbridos de papaya "Tainung-01" y "Scarlett Princess" introducidos en el banco de germoplasma del Instituto de Investigaciones en Fruticultura Tropical, La Habana, Cuba. Y fueron comparados con el cultivar cubano Maradol. Los resultados corroboraron que los híbridos presentan características fenotípicas del Grupo Formosa, frutos de forma elongata en las plantas hermafroditas. El peso medio de los frutos es de 1,6 kg (''Tainung-01'' y 1,7 kg (''Scarlett Princess'', de pulpa color naranja-rojiza y roja, respectivamente, así como, con una productividad entre 98,5 y 63,8 kg.planta-1, características que evidencian la posibilidad de utilizarlos en programas de mejoramiento genético del cultivo y directamente por los productores.

  6. Associação de moscas-das-frutas (Diptera: Tephritidae) com a "meleira do mamoeiro" (Carica papaya L.) / Association of fruit flies (Diptera: Tephritidae) with the sticky disease of papaya (Carica papaya L.)

    Scientific Electronic Library Online (English)

    Antonio S., Nascimento; Walter J., R. Matrangolo; Cristiane J., Barbosa; Oton M., Marques; Tuffi C., Habibe.

    2000-12-01

    Full Text Available [...] Abstract in english Since 1988, the medfly Ceratitis capitata (Wiedemann) was described infesting papaya (Carica papaya) cv. Sunrise Solo in North of the State of Espírito Santo, Brazil. Two experiments were carried out to determine the infestation of C. capitata and Anastrepha obliqua (Macq.) in papaya fruit, healthy [...] and infected by sticky disease. In the 1st experiment (forced infestation), no infestation of C. capitata in the healthy fruits was observed, and 60.6 pupae/fruit were obtained in the infected fruit. For A. obliqua, the infestation index was 10.3 and 72.4 pupae/fruit for healthy and infected fruit respectively. In the 2nd experiment (inoculated eggs), the infestation index for C. capitata was 4.0 and 6.3 pupae/fruit and for A. obliqua 10.1 and 10.4 pupae/fruit, for healthy and infected fruit respectively. The high suscetibility of green fruit of papaya infected by sticky disease for C. capitata and A. obliqua was discussed

  7. Anatomy of somatic embryogenesis in Carica papaya L.

    Directory of Open Access Journals (Sweden)

    Juliana A. Fernando

    2001-09-01

    Full Text Available Mature zygotic embryos of Carica papaya L. ‘Sunrise Solo’ were used as explants for embryogenesis induction. The explants were inoculated on Murashige and Skoog culture medium supplemented with 2 mg.L-1 2,4-dichlorophenoxyacetic acid and incubated in darkness at 25+2°C. Histological analysis of callogenesis and somatic embryogenesis indicated occurrence of direct and indirect somatic embryogenesis development. Direct somatic embryo formation was observed from hypocotyledonary epidermic cells only from explant 18 days after inoculation. Somatic embryos formed indirectly were originated from embryogenic superficial cells of pre-embryonic complexes located on peripherical and on internal cell layers of callus 49 days after inoculation. Diverse morphological differences including disformed embryos were observed among the somatic embryos.Embriões zigóticos maduros de Carica papaya L. ‘Sunrise Solo’ foram utilizados como explantes para indução da embriogênese. Estes explantes foram inoculados em meio de cultura de Murashige & Skoog suplementado com 2,0 mg.L-1 de 2,4 ácido diclorofenoxiacético (2,4-D e mantidos no escuro em câmara de crescimento à temperatura de 21°C por período de tempo variável. Através da análise histológica foi possível verificar que os primeiros embriões somáticos formaram-se diretamente a partir de células únicas da epiderme hipocotiledonar do explante após o 18º dia de cultura. Porém, os demais embriões somáticos originaram-se indiretamente a partir de células superficiais de complexos pré-embriônicos presentes nas camadas periféricas e internas do calo após o 49º dia de cultura. Foram detectadas algumas diferenças morfológicas entre os embriões somáticos obtidos.

  8. Anatomy of somatic embryogenesis in Carica papaya L.

    Scientific Electronic Library Online (English)

    Juliana A., Fernando; Murilo, Melo; Marli K. M., Soares; Beatriz, Appezzato-da-Glória.

    2001-09-01

    Full Text Available Embriões zigóticos maduros de Carica papaya L. ‘Sunrise Solo’ foram utilizados como explantes para indução da embriogênese. Estes explantes foram inoculados em meio de cultura de Murashige & Skoog suplementado com 2,0 mg.L-1 de 2,4 ácido diclorofenoxiacético (2,4-D) e mantidos no escuro em câmara de [...] crescimento à temperatura de 21°C por período de tempo variável. Através da análise histológica foi possível verificar que os primeiros embriões somáticos formaram-se diretamente a partir de células únicas da epiderme hipocotiledonar do explante após o 18º dia de cultura. Porém, os demais embriões somáticos originaram-se indiretamente a partir de células superficiais de complexos pré-embriônicos presentes nas camadas periféricas e internas do calo após o 49º dia de cultura. Foram detectadas algumas diferenças morfológicas entre os embriões somáticos obtidos. Abstract in english Mature zygotic embryos of Carica papaya L. ‘Sunrise Solo’ were used as explants for embryogenesis induction. The explants were inoculated on Murashige and Skoog culture medium supplemented with 2 mg.L-1 2,4-dichlorophenoxyacetic acid and incubated in darkness at 25+2°C. Histological analysis of call [...] ogenesis and somatic embryogenesis indicated occurrence of direct and indirect somatic embryogenesis development. Direct somatic embryo formation was observed from hypocotyledonary epidermic cells only from explant 18 days after inoculation. Somatic embryos formed indirectly were originated from embryogenic superficial cells of pre-embryonic complexes located on peripherical and on internal cell layers of callus 49 days after inoculation. Diverse morphological differences including disformed embryos were observed among the somatic embryos.

  9. Promover la salud mental con mujeres chilenas en desventaja social

    Directory of Open Access Journals (Sweden)

    M Soledad Rivera

    2004-01-01

    Full Text Available Se descrive una parte de la experiencia del proyecto Chileno/Canadiense: ?Promover la salud mental de las mujeres chilenas en desventaja social: Los Secretos de Maruja?. Se describen los objetivos, las etapas del proceso, de intervención y los resultados de las evaluaciones preliminares. Con la participación de las mujeres usuárias de los centros de salud, profesionales y autoridades de la comuna de La Pintana, se construyó, aplicó y evaluó un proyecto de intervención para apoyar a las mujeres en la toma de decisiones que favorezcan su salud mental personal, familiar y social. La intervención contempló el diseño, producción, distribución y evaluación de 12 folletos educativos tipo calendarios, cada uno respecto a tres temas de salud mental identificados por las mismas mujeres: Identidad y autoestima, Vínculo y Comunicación y Autocuidado. Además se involucró a las enfermeras que trabajan en los Centros de Salud, quienes incorporaron esta estrategia a la atención de salud habitual y evaluaron su impacto en la identidad, autonomía profesional y en la mejoría de la calidad del cuidado

  10. Interacciones tempranas y género infantil en familias monoparentales chilenas

    Directory of Open Access Journals (Sweden)

    Marcia Olhaberry Huber

    2012-01-01

    Full Text Available Se realizó un estudio transversal de las interacciones madre-hijo(a en familias monoparentales chilenas de bajo ingresos, considerando las diferencias según el género infantil. Participan 80 díadas, con 39 niñas y 41 niños entre los 4 y 15 meses y se evalúa la calidad de las interacciones con el instrumento CARE-Index. Se observan altos puntajes en depresión y estrés materno, así como un promedio en la calidad de la interacción que indica necesidad de intervención, mostrando lo anterior vulnerabilidad en las díadas del estudio. Las díadas con infantes de género masculino muestran interacciones significativamente más difíciles con sus madres que las de género femenino, quienes a su vez muestran interacciones significativamente más pasivas que los infantes de género masculino. Los tamaños de efecto observados en las diferencias son medianos en ambos casos. Se observa también complementariedad en las conductas de madres e hijos(as, obteniéndose correlaciones positivas y significativas entre sensibilidad materna y cooperatividad infantil, control materno y las conductas difíciles de los infantes, así como entre la conducta no responsiva de las madres y la actitud pasiva en los niños(as. Se discuten los hallazgos a partir de las teorías existentes en relación a género infantil e interacciones tempranas, en el contexto de familias monoparentales a cargo de la madre.

  11. Contrahegemonía y clase trabajadora en una comuna chilena

    Scientific Electronic Library Online (English)

    Mirtha, Lischetti.

    Full Text Available Los procesos globalizadores encuentran en el Estado chileno un campo propicio para desarrollarse después de que la brutal represión de la dictadura militar eliminara la posibilidad de un gobierno socialista llegado al poder por vía electoral. Los contenidos ideológicos de la globalización, así como [...] las políticas económicas concretas que se llevan a la práctica en su nombre, transforman las subjetividades de los sujetos. La clase trabajadora chilena de ideología y tradición mayoritariamente de izquierda queda marginada de la vida política dentro de su propia realidad. Nuestro análisis indaga acerca de los alcances y límites de la supresión de la vida política dentro de dicha clase y la posible construcción de una contrahegemonía que ésta pueda realizar. Abstract in english The Chilean State proved to be fertile ground for globalization processes to develop and unfold once that the brutal repression carried out by the military dictatorship wiped out the possibility for a Socialist government that had come to power through democratic elections. The ideological tenets of [...] globalization, as well as the concrete economic policies that were implemented under such term, transformed the subjectivities of individuals. Chile´s working class, whose ideology and heritage had been traditionally Communist, was left out of the political scene and developments within its own reality. Our analysis delves into the scope, reach, and limits of the suppression of political activities and developments within said class and the possible creation of a counterhegemony that the working class may carry out.

  12. Study about papaya (Carica papaya L.) conservation submitted to a combined treatment of irradiation and an edible film of quitosan

    International Nuclear Information System (INIS)

    In this research, on a first stage the effect of two treatments were studied: thermal, and a chitosan edible coating, combined with four different irradiation doses: 0,00 kGy, 0,50 kGy, 0,75 kGy and 1,00 kGy for effects in the ripening of papaya (Carica Papaya) cv. Golden. To evaluate the influence of these two factors in the physical-chemical characteristics of the fruit during ripening, analysis on color, both skin and pulp, firmness, soluble solids, pH and titrable acidity were performed. The incidence of fungi in fruit skin was also analyzed. The fruits were first maintained at 10 deg C chamber for fifteen days to simulate maritime transportation to the importer country and then transferred to a 25 deg C chamber aiming simulation of the commercialization conditions. From this first step, the conclusion was that chitosan in the concentration of 2% was not able to contain the fungi development and that it also influenced in a significant form the variable pH. A more expressive result was obtained for irradiation doses of 0,75 kGy and 1,00 kGy, as it was observed in the beginning the acceleration in ripening through the variables skin color and penetration energy of the fruit pulp. When storage temperature was changed to 25 deg C this situation inverted for these same variables and a two day delay was observed on the development of yellow color on the fruit skin and loss of pulp firmness. The variables titrable acidity, soluble solids and pulp color were not influenced by treatment or by irradiation. On a second stage, the fruits, in the same condition of the previous stage, were analyzed in relation of their mass and ripening stage using a ripening scale provided by the producer of the fruits for this experiment, as to evaluate the gradual development of fruit ripening according to the various treatments (chitosan 3%, thermal or control) and irradiation dose (0,00 kGy and 0,75 kGy). Confirming the results obtained in the first stage, the irradiation was able to delay ripening in one day and a half approximately. The chitosan in these new conditions of test propitiated fungi development and the fruits coated with chitosan did not ripe in the way it was seen to come. A last stage was done to verify consumer behavior in respect to an irradiated papaya. By three different sensorial tests it was verified if provers were able to perceive significant difference between irradiated and non irradiated samples, what was the acceptance of a irradiated sample and the intention of purchase of a irradiated and a non irradiated papaya. Conclusion from these tests was that the difference established in the triangular test was not enough to establish significant difference between the acceptances of the two samples in the four parameters tested: appearance, texture, aroma and flavor. For the purchase intention it was verified that the irradiation is not a barrier for the consumer. (author)

  13. Subversión de la figura del mártir en la narrativa chilena contemporánea

    Directory of Open Access Journals (Sweden)

    Claire Mercier

    2013-06-01

    Full Text Available El estudio se acerca a la subversión de la figura tradicional del mártir en la narrativa chilena contemporánea, a partir del análisis de dos novelas: El Desierto de Carlos Franz (2005 y La vida doble de Arturo Fontaine (2010. Se inscriben su trama durante el periodo de la Dictadura Chilena y a través del camino identitario de dos mujeres. Así, se tratará de seguir una huella ambigua y torturada respecto a una inversión de la figura del mártir "convencional", a fin de esbozar los contornos de su recreación por la literatura (y para qué.

  14. EFEITOS DO CAPITAL INTELECTUAL SOBRE O DESEMPENHO FINANCEIRO EM EMPRESAS BRASILEIRAS E CHILENAS

    Directory of Open Access Journals (Sweden)

    Salete Turra

    2015-06-01

    Full Text Available O objetivo da pesquisa foi verificar a influência do Capital Intelectual medido pelo Valor Agregado do Capital Intelectual (VACI sobre o desempenho financeiro em empresas brasileiras e chilenas no ano de 2013. Realizou-se uma pesquisa descritiva, documental, com abordagem quantitativa por meio da técnica estatística correlação canônica. Os dados foram coletados na base de dados da Tomnson®. Os resultados do estudo demonstraram correlação canônica existente entre as variáveis que compõem o VACI com as variáveis de desempenho financeiro tanto no Brasil como no Chile. Desse modo, concluiu-se que o capital intelectual influencia significativamente o desempenho financeiro das empresas brasileiras e chilenas.

  15. Use of microsatellite markers in molecular analysis of segregating populations of papaya (Carica papaya L.) derived from backcrossing.

    Science.gov (United States)

    Pinto, F O; Pereira, M G; Luz, L N; Cardozo, D L; Ramos, H C C; Macedo, C M P

    2013-01-01

    Brazil is the world leader in papaya production. However, only a small number of cultivars are registered for commercial planting, mainly owing to delays in obtaining cultivars and the high costs of the field phase of breeding programs. These costs can be reduced when molecular tools are combined with conventional breeding methods. In the present study, we conducted a molecular analysis of a self-fertilized population of a first backcrossing generation of BC1S1 papaya plants via microsatellite markers both to monitor the level of homozygosity and the gene/allele transfer that confers the Golden trait (fruit color) and to assess the parental genomic proportion in the genotypes studied. Based on the analysis of 20 polymorphic microsatellite loci, 19 genotypes with the Golden trait belonging to BC1S1 were evaluated in addition to the parental genotypes. Genetic distance was estimated through weighted index. The genotypes were then grouped using the hierarchical nearest neighbor method, and the analysis of principal coordinates was used to measure the proportion of parental genomes in the segregating genotypes. The mean value of the inbreeding coefficient was 0.36. The analysis of the principal coordinates revealed that on average, 64% of the recurrent parent genome was present in the population. Together, the analyses allowed the selection of 3 individuals for the next backcross cycle (33BC1S1-18, 34BC1S1-16, and 37BC1S1-10). These individuals had a higher proportion of the recurrent parent and were grouped close to the recurrent parent in the cluster analysis. PMID:23884768

  16. Molecular diagnosis of Papaya meleira virus (PMeV) from leaf samples of Carica papaya L. using conventional and real-time RT-PCR.

    Science.gov (United States)

    Abreu, Paolla M V; Piccin, João G; Rodrigues, Silas P; Buss, David S; Ventura, José A; Fernandes, Patricia M B

    2012-03-01

    Papaya meleira virus (PMeV) is the causal agent of papaya sticky disease. This study describes two methods for molecular diagnosis of PMeV using conventional and real-time PCR. These methods were shown to be more efficient than current methods of viral detection using extraction of PMeV dsRNA and observation of symptoms in the field. The methods described here were used to evaluate the effect of inoculation of papaya plants with purified PMeV dsRNA on the progress of PMeV infection. A single inoculation with PMeV dsRNA was observed to delay the progress of the virus infection by several weeks. The possibility of vertical transmission of PMeV was also investigated. No evidence was found for PMeV transmission through seeds collected from diseased fruit. The implications of these results for the epidemiology of PMeV and the management of papaya sticky disease are discussed. PMID:22193169

  17. Recombinant pro-regions from papain and papaya proteinase IV-are selective high affinity inhibitors of the mature papaya enzymes.

    Science.gov (United States)

    Taylor, M A; Baker, K C; Briggs, G S; Connerton, I F; Cummings, N J; Pratt, K A; Revell, D F; Freedman, R B; Goodenough, P W

    1995-01-01

    Proteolytic enzymes require the presence of their pro-regions for correct folding. Of the four proteolytic enzymes from Carica papaya, papain and papaya proteinase IV (PPIV) have 68% sequence identity. We find that their pro-regions are even more similar, exhibiting 73.6% identity. cDNAs encoding the pro-regions of these two proteinases have been expressed in Escherichia coli independently from their mature enzymes. The recombinant pro-regions of papain and PPIV have been shown to be high affinity inhibitors of all four of the mature native papaya cysteine proteinases. Their inhibition constants are in the range 10(-6) - 10(-9) M. PPIV was inhibited two to three orders of magnitude less effectively than papain, chymopapain and caricain. The pro-region of PPIV, however, inhibited its own mature enzyme more effectively than did the pro-region of papain. Alignment of the sequences of the four papaya enzymes shows that there is a highly variable section towards the C-terminal of the pro-region. This region may therefore confer selectivity to the pro-regions for the individual proteolytic enzymes. PMID:7770454

  18. 76 FR 49725 - Notice of Decision To Authorize the Importation of Fresh Papaya Fruit From Malaysia into the...

    Science.gov (United States)

    2011-08-11

    ..., 2011 (76 FR 13972, Docket No. APHIS-2011- 0013), in which we announced the availability, for review and... Importation of Fresh Papaya Fruit From Malaysia into the Continental United States AGENCY: Animal and Plant... authorize the importation into the continental United States of fresh papaya fruit from Malaysia. Based...

  19. 76 FR 13972 - Notice of Availability of a Pest Risk Analysis for the Importation of Fresh Papaya Fruit From...

    Science.gov (United States)

    2011-03-15

    ...of Fresh Papaya Fruit From Malaysia Into the Continental United...Carica papaya) fruit from Malaysia into the continental United...programs is available on the Internet at http://www.aphis...request from the Government of Malaysia to allow the importation...

  20. Planting spacing and NK fertilizing on physiological indexes and fruit production of papaya under semiarid climate

    Directory of Open Access Journals (Sweden)

    Eduardo Monteiro Santos

    2015-01-01

    Full Text Available ABSTRACT The nutritional requirements of papaya (Carica papaya L. increase continuously throughout the crop cycle, especially for potassium and nitrogen, which are the most required nutrients and act on plant vital functions such as photosynthetic activity, respiration, transpiration and stomatal regulation. An experiment was conducted from November 2010 to December 2012 to evaluate physiological indexes and fruit production of papaya cv. Caliman-01 as a function of planting spacing and NK fertilizing. The experimental design consisted of randomized blocks, with treatments distributed in a factorial arrangement (2 × 4 × 4, using 2 planting spacing [simple rows (3.8 × 2.0 m and double rows (3.8 × 2.0 × 1.8 m], 4 nitrogen doses (320, 400, 480 and 560 g of N per plant-1 and 4 potassium doses (380, 475, 570 and 665 g of K2O per plant-1 with 4 replications of 3 plants each. The following variables were evaluated: leaf area index (LAI, leaf chlorophyll index (a, b and total index, intercepted photosynthetically active radiation (Int.PAR, in µmol?m-2?s-1, efficiency use of photosynthetically active radiation (Ef.PAR and fruit yield. The fruit production and physiological characteristics of papaya cv. Caliman-01 depend on planting spacing. Under the soil, climate and plant conditions of this study, 665 g of K2O and 320 g of N per plant under double spacing could be recommended for the production of papaya cv. Caliman-01.

  1. Phytochemical screening and hypoglycemic activity of Carica papaya leaf in streptozotocin-induced diabetic rats

    Directory of Open Access Journals (Sweden)

    Isela E. Juárez-Rojop

    2014-06-01

    Full Text Available The extraction of plant constituents is essential to isolate biologically active compounds, aimed to understand their role on the treatment of diabetes. This study was designed to explore the preliminary phytochemical and physicochemical analysis of Carica papaya L., Caricaceae, leaf, and further evaluation of its hypoglycemic effect on diabetic rats. C. papaya leaves were extracted using chloroform, n-hexane or ethanol. For each extract a phytochemical screening was performed. The tests were conducted in triplicate and the qualitative and quantitative determination of the various metabolites was done using analytical standards proposed by Mexican Herbal Pharmacopoeia. The chloroform extract, containing steroids and quinones as major components, was chosen to study C. papaya biological effects. The chloroform extract was evaporated to dryness, and doses 0, 31, 62, 125 mg/kg were orally administered in 300 µl polyethylene glycol to diabetic rats; and 0 and 62 mg/kg to non-diabetic rats. After a 20-day treatment with the chloroform extract, the animals were sacrificed and blood was obtained for biochemical studies. The main effect observed was a decrease in serum glucose, triglycerides and transaminases in diabetic rats after the administration of C. papaya chloroform extract. These results confirm the potential beneficial action of C. papaya to treat the symptoms of diabetic patients.

  2. Eradication of Bactrocera papayae (Diptera: Tephritidae) by male annihilation and protein baiting in Queensland, Australia

    International Nuclear Information System (INIS)

    An established population of Bactrocera papayae Drew and Hancock (Asian papaya fruit fly) was detected on the Australian mainland near Cairns, north Queensland, on 16 October 1995 (Fay et al. 1997). Coincidentally, the first flies were bred from green papaya, a host not normally utilised in the unripe state by indigenous fruit fly species. B. papayae is a member of the Oriental fruit fly (Bactrocera dorsalis) complex (Drew and Hancock 1994) and has more than 190 known hosts in Southeast Asia, where it is a major pest species. Consequently, the threat to Australian horticulture posed by the incursion was recognised immediately. By 26 October 1995, breeding populations were confirmed around Cairns, Mossman and Mareeba and a Pest Quarantine Area (PQA) was legislated. By 1 November 1995, following detections south of Innisfail, the PQA boundaries were set at 144 deg. 15'E, 19 deg. 00'S, covering nearly 78,000 km2. B. papayae was ultimately trapped over some 20,000 km2. A national eradication campaign began in November 1995, undertaken by the Queensland Department of Primary Industries

  3. Phytochemical screening and hypoglycemic activity of Carica papaya leaf in streptozotocin-induced diabetic rats

    Scientific Electronic Library Online (English)

    Isela E., Juárez-Rojop; Carlos, A.Tovilla-Zárate; Dora E., Aguilar-Domínguez; Luis F. Roa-de la, Fuente; Carlos E., Lobato-García; Jorge L., Blé-Castillo; Leonor, López-Meraz; Juan C., Díaz-Zagoya; Deysi Y., Bermúdez-Ocaña.

    2014-06-01

    Full Text Available The extraction of plant constituents is essential to isolate biologically active compounds, aimed to understand their role on the treatment of diabetes. This study was designed to explore the preliminary phytochemical and physicochemical analysis of Carica papaya L., Caricaceae, leaf, and further ev [...] aluation of its hypoglycemic effect on diabetic rats. C. papaya leaves were extracted using chloroform, n-hexane or ethanol. For each extract a phytochemical screening was performed. The tests were conducted in triplicate and the qualitative and quantitative determination of the various metabolites was done using analytical standards proposed by Mexican Herbal Pharmacopoeia. The chloroform extract, containing steroids and quinones as major components, was chosen to study C. papaya biological effects. The chloroform extract was evaporated to dryness, and doses 0, 31, 62, 125 mg/kg were orally administered in 300 µl polyethylene glycol to diabetic rats; and 0 and 62 mg/kg to non-diabetic rats. After a 20-day treatment with the chloroform extract, the animals were sacrificed and blood was obtained for biochemical studies. The main effect observed was a decrease in serum glucose, triglycerides and transaminases in diabetic rats after the administration of C. papaya chloroform extract. These results confirm the potential beneficial action of C. papaya to treat the symptoms of diabetic patients.

  4. Effectiveness of dried Carica papaya seeds against human intestinal parasitosis: a pilot study.

    Science.gov (United States)

    Okeniyi, John A O; Ogunlesi, Tinuade A; Oyelami, Oyeku A; Adeyemi, Lateef A

    2007-03-01

    The tropical fruit Carica papaya and its seeds have proven antihelminthic and anti-amoebic activities. To determine the effectiveness of air-dried C. papaya seeds on human intestinal parasitosis, 60 asymptomatic Nigerian children with stool microscopic evidence of intestinal parasites received immediate doses (20 mL) of either an elixir composed with air-dried C. papaya seeds and honey (CPH) or honey alone (placebo) in two randomized treatment groups. Repeat stool microscopic examinations were conducted 7 days postintervention for intestinal parasites. Significantly more subjects given CPH elixir than those given honey had their stools cleared of parasites [23 of 30 (76.7%) vs. five of 30 (16.7%); z = 4.40, P = .0000109]. There were no harmful effects. The stool clearance rate for the various types of parasites encountered was between 71.4% and 100% following CPH elixir treatment compared with 0-15.4% with honey. Thus, air-dried C. papaya seeds are efficacious in treating human intestinal parasites and without significant side effects. Their consumption offers a cheap, natural, harmless, readily available monotherapy and preventive strategy against intestinal parasitosis, especially in tropical communities. Further and large-scale intervention studies to compare C. papaya with standard antiparasitic preparation are desirous. PMID:17472487

  5. Carbon disulfide formation in papaya under conditions of dithiocarbamate residue analysis.

    Science.gov (United States)

    Abakerli, R B; Sparrapan, R; Sawaya, A C H F; Eberlin, M N; Jara, J L P; Rodrigues, N R; Fay, E F; Luiz, A J B; Galvão, T D L; Martins, D dos S; Yamanishi, O K; Toledo, H H B

    2015-12-01

    Golden, Sunrise Solo and Tainung cultivars of papaya were found to release CS2 when submitted to experimental conditions of dithiocarbamate residue analysis. Three common analytical methods were used to quantitate CS2; one spectrophotometric method and two chromatographic methods. All three methods gave positive CS2 results for all three papaya varieties. Other endogenous compounds present in isooctane extracts of papaya fractions detected via gas chromatography (GC/ITD) using electron ionization (EI) were: carbonyl sulfide, dimethyl sulfide, carbon disulfide, 2-methylthiophene, 3-methylthiophene, 2-ethylthiophene, 3-ethylthiophene, benzylisothiocyanate, benzylthiocyanate and benzonitrile. Control samples were obtained from papaya plantations cultivated in experimental areas, in which no treatment with fungicides of the dithiocarbamate group was applied. Endogenous CS2 levels were compared with true dithiocarbamate residues measured in papaya samples from the field trials following applications of the mancozeb fungicide. Three days after application, true dithiocarbamate residues, measured by the procedure with isooctane partitioning and GC-ITD, were at the average level of 2 mg kg(-1). PMID:26041166

  6. KURSUS SINGKAT DAN PELATIHAN PEMANFAATAN PEPAYA (CARISA PAPAYA L. MENJADI MANISAN KERING BUAH PEPAYA DI DESA TIMUHUN, KECAMATAN BANJARANGKAN, KABUPATEN KLUNGKUNG

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    I Made Nada

    2012-09-01

    Full Text Available ABSTRACT In Desa Timuhun, eventhough papaya is not primary fruit product, but usually papaya is planted among the other plants and easily to grow. Papaya has good potency to be processed to become food product. Matured papaya fruit is usually fresh served for desk fruit. On the other hand, for papaya which is not mature yet, usually used for vegetable. Papaya has high water content, so it makes papaya fruit easy to experience damage. With papaya processing, papaya which is originally assumed improper to be sold or to be consumed (mechanical damage, diseased, acid, physical damage and will only to be thrown, finally can be used. The making of papaya dry candy is one of effort to preserve and to vary form of papaya serving. It also improves the economic value of papaya. The making of papaya can be conducted in big or small scale. The activity objectives are to introduce and give additional knowledge to society about papaya processing technology, to increase economic added value of papaya and the using of papaya which is improper to be sold or to be consumed becoming valuable food product .Realization of the objectives above are by performing an information sharing and discussion with the society about process of the making dry candy from papaya, training of papaya dry candy making, introduce simple technology of dry candy papaya making. This activity was performed at the head of Desa Timuhun office, on Friday, September 14, 2007 and was attended by 26 persons, including association of woman farmer and the staff in that office. The result of this activity shows that there was positive response of people who attend this activity, because by processing papaya fruit into dry candy, have a lot of advantage compare to sale it freshly. Beside that, this dry candy is healthy snack, without synthetic preservation, enriched with vitamins which is useful for health, also this dry candy is still good up to 1 month and of course it could to improve economic value compared to be sold freshly

  7. ANÁLISIS DE CONSULTAS A UN BUSCADOR DE LA WEB CHILENA

    Scientific Electronic Library Online (English)

    Ricardo, Baeza-Yates; Carolina, Galleguillos.

    2005-04-01

    Full Text Available Los buscadores de páginas Web son herramientas indispensables de orientación para sus usuarios, lo cual refleja la gran cantidad de personas que los utilizan diariamente. Las bitácoras (logs) que registran el uso de un buscador son una valiosa fuente de información: nos hablan de las expectativas, p [...] referencias y necesidades de las personas, es decir, qué esperan ellas encontrar en la Web. El resultado del análisis de logs de uso tiene un importante valor comercial, en particular para el comercio electrónico y la publicidad en Internet. El objetivo de este estudio es encontrar relaciones entre las consultas de los usuarios de la Web Chilena (.cl) y las diversas áreas de clasificación de sus diferentes sitios. También se esperan encontrar relaciones entre los tipos de búsqueda realizados y los sitios visitados, para así conocer la necesidad que los lleva a formular sus consultas. La información obtenida está basada en los logs del buscador chileno TodoCL [1] entre febrero y marzo de 2004. Abstract in english Web page search engines are essential tools on users’ orientation, which is reflected by the big amount of people who are using them every day. The logs, reflecting the Web search engine uses, are a valuable source of information: they give us information about the users’ expectations, preferences a [...] nd needs, which is everything that they expect to find on the Web. The results from the logs usage analysis have an important commercial value, in particular, for e-commerce and Internet publicity. The purpose of this study is to find relations between Chilean Web (.cl) users’ queries and the different classification areas for its web sites. Also we expect to find relations between the search types made and the web sites visited, trying to get the need behind the query . The data used for this study was gotten from the Chilean search engine TodoCL, between February and March 2004.

  8. ANÁLISIS DE CONSULTAS A UN BUSCADOR DE LA WEB CHILENA

    Directory of Open Access Journals (Sweden)

    Ricardo Baeza-Yates

    2005-04-01

    Full Text Available Los buscadores de páginas Web son herramientas indispensables de orientación para sus usuarios, lo cual refleja la gran cantidad de personas que los utilizan diariamente. Las bitácoras (logs que registran el uso de un buscador son una valiosa fuente de información: nos hablan de las expectativas, preferencias y necesidades de las personas, es decir, qué esperan ellas encontrar en la Web. El resultado del análisis de logs de uso tiene un importante valor comercial, en particular para el comercio electrónico y la publicidad en Internet. El objetivo de este estudio es encontrar relaciones entre las consultas de los usuarios de la Web Chilena (.cl y las diversas áreas de clasificación de sus diferentes sitios. También se esperan encontrar relaciones entre los tipos de búsqueda realizados y los sitios visitados, para así conocer la necesidad que los lleva a formular sus consultas. La información obtenida está basada en los logs del buscador chileno TodoCL [1] entre febrero y marzo de 2004.Web page search engines are essential tools on users’ orientation, which is reflected by the big amount of people who are using them every day. The logs, reflecting the Web search engine uses, are a valuable source of information: they give us information about the users’ expectations, preferences and needs, which is everything that they expect to find on the Web. The results from the logs usage analysis have an important commercial value, in particular, for e-commerce and Internet publicity. The purpose of this study is to find relations between Chilean Web (.cl users’ queries and the different classification areas for its web sites. Also we expect to find relations between the search types made and the web sites visited, trying to get the need behind the query . The data used for this study was gotten from the Chilean search engine TodoCL, between February and March 2004.

  9. Para una entrada en la imaginación poética alimentaria chilena

    Scientific Electronic Library Online (English)

    Magda, Sepúlveda.

    2014-04-01

    Full Text Available Las comidas y las bebidas recreadas en la poesía chilena ofrecen un espacio alternativo para reflexionar sobre la etnia, la clase social, el género y la violencia política. Cada uno de estos nudos define una ruta alimenticia donde las historias diferenciales se toman la palabra. Este artículo propon [...] e una cartografía de las imágenes alimentarias poéticas, caracterizando cuatro grandes rutas: aquellas donde la escritura privilegia la construcción de comunidades indígenas alrededor de la comida y aquellas donde los proyectos estéticos elaboran un patrimonio provinciano y/o rural alrededor de las comidas como defensa frente a las legitimidades centristas. Más aquellos itinerarios cuyas simbolizaciones erigen o deconstruyen los deseos caníbales entre diversas subjetividades y; aquellas donde los linajes lingüísticos hacen del hambre un motivo de descontento político, ya sea imaginando la abundancia a través del tópico de la tierra de Jauja o elaborando el hambre de los dioses que piden sacrificios humanos. Abstract in english Food and drink recreated in the Chile an poetry offer an alternative space to reflect upon ethnic matters, social class, gender and political violence. Every topoi defines a food route where stories have their say. In this way, we find four main routes: those in which writing priviledges the constru [...] ction of native communities around food preparation; those in which aesthetical projects elaborate their rural patrimony around food to defend themselves from centralized domination; those whose symbols construct and deconstruct cannibalistic desires among different intersubjectivities and those whose linguistic ancestry make hunger a motif of political discontent either by imagining abundance in the land of milk and honey or by elaborating the hunger of gods who demand human sacrifices.

  10. Estética, política y vida cotidiana. El caso de la pintura callejera chilena

    Directory of Open Access Journals (Sweden)

    Patricio Rodriguez-Plaza

    2005-06-01

    Full Text Available Este artículo trata de compartir una reflexión académica con respecto al fenómeno de la pintura callejera chilena, entendiéndola como un hecho estético total, que lo es menos por sus producciones y resultados que por los usos y percepciones que lo entretejen, conforman y desparraman por la ciudad.

  11. Comparative activity of antioxidants from wheat sprouts, Morinda citrifolia, fermented papaya and white tea.

    Science.gov (United States)

    Calzuola, Isabella; Gianfranceschi, Gian Luigi; Marsili, Valeria

    2006-01-01

    Hydroalcoholic extracts from wheat sprouts, white tea, Morinda citrifolia and fermented papaya were analysed to determine their reducing power and antioxidant activity. The results show that the micromoles of potassium ferricyanide reduced by a quantity of extract corresponding to 1 g of the various dehydrated starting tissues are: 12.91+/-0.83 (wheat sprouts), 10.66+/-1.22 (M. citrifolia), 17.06+/-1.24 (white tea), and 1.05+/-0.09 (fermented papaya). In addition the results show a strong oxygen superoxide scavenging activity in the extracts from white tea, M. citrifolia and wheat sprouts. The activity of the fermented papaya extract is the lowest. The thin-layer chromatography and UV spectrophotometry of the extracts show in each source a mixture of antioxidant compounds probably belonging to the families of reducing glycosides and polyphenols. The chromatographic pattern of the antioxidant compounds and the UV spectrum are quite different in the various sources. PMID:17127467

  12. Dynamic transposable element accumulation in the nascent sex chromosomes of papaya.

    Science.gov (United States)

    Vanburen, Robert; Ming, Ray

    2013-01-01

    From their inception, Y chromosomes in plants and animals are subjected to the powerful effects of Müller's ratchet, a process spurred by suppression of recombination that results in a rapid accumulation of mutations and repetitive elements. These mutations eventually lead to gene loss and degeneration of the Y chromosome. Y chromosomes in mammals are ancient, whereas most sex chromosomes in plants and many in insects and fish evolved recently. Sex type in papaya is controlled by a pair of nascent sex chromosomes that evolved around 7 million years ago. The papaya X and Y(h) were recently sequenced, providing valuable insight into the early stages of sex chromosome evolution. Here we discuss the fruits of this work with a focus on the repeat accumulation, gene trafficking and promiscuous DNA sequences found in the slowly degenerating Y(h) chromosome of papaya. PMID:23734293

  13. The effect of gamma radiation on the chemical content, texture and shelf life of papaya

    International Nuclear Information System (INIS)

    Commercially matured papaya, Carica papaya var. Solo were gamma irradiated at doses of 0 kGy, 0.75 kGy, 1.0 KGy and 1.5 kGy. The production rate of ethylene, development of ripening colour, pectin substances and ascorbic acid content as well as the weight losses were studied during storage at room temperature. Ethylene production, development of ripening colour and softening were found to be retarded by irradiation. Irradiation also slowed down the reduction rate of alcohol insoluble substances and hydrochloric acid soluble pectin and the increment of water soluble pectin and hexamataphosphate soluble pectin. Doses of 1.0 kGy and 1.5 kGy effectively suppressed the synthesis of vitamin C although no effect on the existing ascorbic acid was detected. The weight losses of papaya were not affected by irradiation treatment

  14. Combined Treatment of UV and Gamma Radiation of Papaya for Decay Control

    International Nuclear Information System (INIS)

    An exploratory study was made to determine if combining u.v. and gamma radiation treatment at selective doses would decrease the fungal decay incidence in papaya (Carica papaya L. var. Solo), and thereby increase the marketable life of the fruit. Also studied was the effect of the combined treatment on the spores of Aschochyta spp., Colletotrichium spp. and Phytophthora spp., which are commonly found on papaya grown in Hawaii. This was to test the postulate that the combined treatment would prevent photoreactivation of the disrupted nuclei in microorganisms and lead to eventual death. Experimental results did not indicate a conclusive trend to effectiveness but suggested a number of problems to be resolved before the treatment can become useful in fungal decay control of fruits. (author)

  15. Efecto del etileno sobre el ACC y ACC oxidasa en la maduración de papaya 'maradol' / Ethylene effect on ACC content and ACC oxidase during ripening of 'maradol' papaya

    Scientific Electronic Library Online (English)

    Javier De la, Cruz; Gilber, Vela; Lidia, Dorantes; Hugo S., García.

    2010-06-01

    Full Text Available Frutos de papaya 'Maradol' (Carica papaya L.) fueron tratados con etileno en aire sintético (0, 100, 300 y 500 µL L-1) en contenedores de fibra de vidrio a diferentes tiempos de exposición (8, 16 y 24 h). Después del tratamiento, los frutos fueron transferidos para su maduración a temperatura ambien [...] te (25 °C). Los análisis efectuados en cada tratamiento fueron: firmeza, azúcares reductores, sólidos solubles, acidez titulable, color de cascara y pulpa (Hue), velocidad de producción de etileno (VPE), contenido de ácido 1-aminociclopropano-1-carboxílico (ACC) y actividad de la enzima formadora de etileno (ACC oxidasa). Los resultados indicaron que el primer cambio notable fue el color en la cascara (amarillo) y en la pulpa (naranja), típicos en un fruto listo para el consumo. Los frutos expuestos a 500 µL L-1 de etileno por 16 y 24 h mostraron un pico climatérico un día después del tratamiento, mientras que los frutos expuestos por solamente 8 h mostraron su pico climatérico 2 d después de la exposición al etileno. Las papayas expuestas a 500 µL L-1 de etileno por 16 y 24 h produjeron bajos valores de Hue en menos tiempo que el testigo, y su contenido de ACC aumentó a 2.7 nmol g-1 h-1, la actividad de la ACC oxidasa a 3 nL g-1 h-1, y la velocidad de producción de etileno (VPE) a 24 nL g-1 h-1. Estas condiciones permitieron que se alcanzaran valores de firmeza de 5.2 kgf cm-2 y 12 % de sólidos solubles a los 6 d a 25 °C. El contenido de azúcares reductores, valores de pH y la acidez no mostraron diferencias significativas (P > 0.05) entre tratamientos. Los frutos testigo alcanzaron valores similares después de 8 d de maduración directa. Los resultados sugieren que es posible acelerar la maduración de papaya 'Maradol' de 8 a 3 d por exposición a atmósferas con etileno. Abstract in english 'Maradol' papaya (Carica papaya L.) fruits were subjected to atmospheres containing certified mixtures of synthetic air and ethylene (0, 100, 300 and 500 µL L-1) in fiberglass containers for different periods (8, 16 and 24 h). After exposure, fruits were transferred to ambient conditions (25 °C) for [...] ripening. The analyses performed on each treatment were: firmness, soluble solids content, reducing sugars content, titratable acidity, peel and flesh color (Hue), ethylene production rate (EPR), 1-aminocyclopropane-1-carboxylic acid (ACC) content and ethylene-forming enzyme (ACC oxidadese) activity. Results showed that the first noticeable change in fruits was the yellow and red color turning in peel and flesh respectively, both typical of a fully ripe and ready-to-eat-papaya fruit. Fruits exposed to 500 µL L-1 of ethylene for 16 and 24 h showed a climacteric peak one day after treatment, while fruits exposed for only 8 h displayed their climacteric peak 2 d after ethy-lene exposure. Papayas treated for 16 and 24 h developed very low Hue values in a shorter time then the control, and their ACC content increased to 2.7 nmol g-1 h-1. ACC oxidase activity also increased to 3 nL g-1 h-1, and EPR reached 24 nL g-1 h-1. This set of conditions allowed fruits to reach firmness values of 5.2 kgf cm-2 and 12 % of soluble solids after 6 d at 25 °C. Reducing sugars content and percent age of titratable acidity were not different (P > 0.05) between treatments. Similar values were found in control fruits after 8 d at normal ripening conditions. Results suggest that it is possible to shorten ripening of 'Maradol' papaya fruit from 8 to 3 d after application of exogenous ethylene.

  16. Dinámica del crecimiento de papaya por efecto de la inoculación micorrízica y fertilización con fósforo / Growth dynamics of papaya due to mycorrhizal inoculation and phosphorous fertilization

    Scientific Electronic Library Online (English)

    Evangelina Esmeralda, Quiñones-Aguilar; Luis, López-Pérez; Gabriel, Rincón-Enríquez.

    2014-08-01

    Full Text Available En México, la papaya es un fruto importante de exportación; sin embargo, este mercado exige calidad bio, constituyendo un reto en la producción con el uso de tecnologías bio-ecológicas y sostenibles. Los hongos micorrízicos arbusculares (HMA) pueden contribuir significativamente en la nutrición vege [...] tal, en especial en la obtención de fósforo (P). El empleo de los HMA en papaya es alentador, pero falta definir aspectos sobre el manejo agronómico de productos a base de HMA. El objetivo de este estudio fue evaluar el efecto del HMA Glomus sp. Zac-2 y la fertilización con P, sobre el crecimiento de papaya var. Cera, durante la etapa de vivero. Para ello, se realizó un experimento trifactorial 2 x 3 x 4 (inoculación HMA, fuente y dosis de P) en un diseño completamente al azar. Las variables evaluadas fueron altura de planta (AP) y diámetro de tallo (DT), cada 15 días; biomasa seca (BS) y densidad de esporas (DE), al final del experimento (90 días). A partir de la BS de plantas con y sin micorrizar, se calculó el índice relativo de dependencia micorrízica (IRDM). Las variables AP, DT y BS fueron diferentes (P ? 0.05) entre plantas con y sin HMA, independientemente de la dosis y fuente de P. A los 75 días, las plantas con HMA incrementaron su crecimiento más de 500 % con respecto a las no inoculadas; el IRDM fue 99 %. Estos resultados sugieren que la inoculación de HMA en papaya durante la fase de vivero puede beneficiar su crecimiento posterior en campo. Abstract in english Papaya is a major fruit export for Mexico; however, the international market is increasingly demanding bio quality, representing a significant production challenge due to the need for bio-ecological and sustainable technologies. Arbuscular mycorrhizal fungi (AMF) can contribute significantly to plan [...] t nutrition, particularly in phosphorous (P) uptake. The use of AMF in papaya is yielding encouraging results, but there is a need to define certain aspects regarding agronomic management of AMF-based products. The aim of this study was to evaluate the effect of AMF Glomus sp. Zac-2 and P fertilization on the growth of Cera var. papaya during the nursery stage. For this, a 2 x 3 x 4 tri-factorial experiment (AMF inoculation, P source and P dose) was conducted in a completely randomized design. The variables plant height (PH) and stem diameter (SD) were evaluated every 15 days; dry biomass (DB) and spore density (SD) were assessed at the end of the experiment (90 days). From the DB of plants with and without mycorrhizal inoculation, the relative mycorrhizal dependency index (RMDI) was calculated. Data analysis showed significant differences (P ? 0.05) between papaya plants with and without AMF for PH, SD and DB, regardless of the P dose and source. At 75 days, growth increases of more than 500 % were recorded in plants with AMF compared to the non-inoculated plants; the RMDI was 99 %. These results suggest that AMF inoculation in papaya during the nursery phase can benefit further growth in the field.

  17. Use of morpho-agronomic traits and DNA profiling for classification of genetic diversity in papaya.

    Science.gov (United States)

    de Jesus, O N; de Freitas, J P X; Dantas, J L L; de Oliveira, E J

    2013-01-01

    We examined the genetic diversity of papaya (Carica papaya) based on morpho-agronomic and molecular data. Twenty-seven genotypes grown in Brazil were analyzed with 11 AFLP primer combinations, 23 ISSR markers, 22 qualitative, and 30 quantitative descriptors. For the joint analyses, we used the Gower algorithm (Joint Gower) and the average value of the individual dissimilarity matrix for each type of data (Average-Joint Gower); 359 AFLP and 52 ISSR polymorphic bands were found. Approximately 29.2 and 7.7% of the AFLP and ISSR bands, respectively, were genotype-specific and may therefore be used for papaya variety protection. Although there was a significant correlation between the qualitative and quantitative descriptor dissimilarity matrices (r = 0.43), the morpho-agronomic data were not highly correlated with the molecular data. Moreover, correlation between AFLP and ISSR dissimilarity matrices was nearly null (r = -0.01). Joint Gower analysis of all data showed high correlations, especially for AFLP markers, most likely due to the larger number of bands, generating a strong bias in the diversity estimates. The Average-Joint Gower analysis allowed a better balance between the correlations for the continuous and the discrete variables. The results generated by clustering analysis distinguished 5 genetically distinct groups. While we found that papaya genotypes are significantly variable for many traits, we observed that Average-Joint Gower analysis allowed for genotype clustering based on the most widely used criterion for classifying papaya genotypes, which is fruit type ('Formosa' or 'Solo'). This information helps provide an accurate estimate of the genetic diversity and structure of papaya germplasm, which will be used for further breeding strategies. PMID:23546977

  18. Stability and efficiency of dye-sensitized solar cells based on papaya-leaf dye

    Science.gov (United States)

    Suyitno, Suyitno; Saputra, Trisma Jaya; Supriyanto, Agus; Arifin, Zainal

    2015-09-01

    The present article reports on the enhancement of the performance and stability of natural dye-based dye-sensitized solar cells (DSSCs). Natural dyes extracted from papaya leaves (PL) were investigated as sensitizers in TiO2-based DSSCs and evaluated in comparison with N719 dye. The acidity of the papaya-leaf extract dyes was tuned by adding benzoic acid. The TiO2 film-coated fluorine-doped tin oxide glass substrates were prepared using the doctor-blade method, followed by sintering at 450 °C. The counter electrode was coated by chemically deposited catalytic platinum. The working electrodes were immersed in N719 dye and papaya dye solutions with concentrations of 8 g/100 mL. The absorbance spectra of the dyes were obtained by ultra-violet-visible spectroscopy. The energy levels of the dyes were measured by the method of cyclic voltammetry. In addition, Fourier transform infrared spectroscopy was used to determine the characteristic functionalities of the dye molecules. The DSSC based on the N719 dye displayed a highest efficiency of 0.87% whereas those based on papaya-leaf dye achieved 0.28% at pH 3.5. The observed improved efficiency of the latter was attributed to the increased current density value. Furthermore, the DSSCs based on papaya-leaf dye with pH 3.5-4 exhibited better stability than those based on N719 dye. However, further studies are required to improve the current density and stability of natural dye-based DSSCs, including the investigation of alternative dye extraction routes, such as isolating the pure chlorophyll from papaya leaves and stabilizing it.

  19. Stability and efficiency of dye-sensitized solar cells based on papaya-leaf dye.

    Science.gov (United States)

    Suyitno, Suyitno; Saputra, Trisma Jaya; Supriyanto, Agus; Arifin, Zainal

    2015-09-01

    The present article reports on the enhancement of the performance and stability of natural dye-based dye-sensitized solar cells (DSSCs). Natural dyes extracted from papaya leaves (PL) were investigated as sensitizers in TiO2-based DSSCs and evaluated in comparison with N719 dye. The acidity of the papaya-leaf extract dyes was tuned by adding benzoic acid. The TiO2 film-coated fluorine-doped tin oxide glass substrates were prepared using the doctor-blade method, followed by sintering at 450 °C. The counter electrode was coated by chemically deposited catalytic platinum. The working electrodes were immersed in N719 dye and papaya dye solutions with concentrations of 8 g/100 mL. The absorbance spectra of the dyes were obtained by ultra-violet-visible spectroscopy. The energy levels of the dyes were measured by the method of cyclic voltammetry. In addition, Fourier transform infrared spectroscopy was used to determine the characteristic functionalities of the dye molecules. The DSSC based on the N719 dye displayed a highest efficiency of 0.87% whereas those based on papaya-leaf dye achieved 0.28% at pH 3.5. The observed improved efficiency of the latter was attributed to the increased current density value. Furthermore, the DSSCs based on papaya-leaf dye with pH 3.5-4 exhibited better stability than those based on N719 dye. However, further studies are required to improve the current density and stability of natural dye-based DSSCs, including the investigation of alternative dye extraction routes, such as isolating the pure chlorophyll from papaya leaves and stabilizing it. PMID:25875031

  20. Papaya extract to treat dengue: a novel therapeutic option?

    Science.gov (United States)

    Sarala, N; Paknikar, Ss

    2014-05-01

    Dengue is a viral disease that today affects a vast number of people in over 125 countries and is responsible for a sizable number of deaths. In the absence of an effective antiviral drug to treat the disease, various treatments are being investigated. Studies have indicated that the juice of the leaves of the Carica papaya plant from the family Caricaceae could help to increase the platelet levels in these patients. This review describes some of the published studies on this topic. The search was done independently by the two authors using PubMed, Google and the library database and included relevant articles of the last 10 years. A total of 7 studies were included in this review, which were one animal study, one case report, three case series and two randomized controlled trials. Although many of the studies and case reports published in literature lack adequate information, some of the studies do raise the possibility that this treatment could be an important option in the future. Further large-scale studies could establish the usefulness or ineffectiveness of this natural product in the treatment of dengue. PMID:24971201

  1. Potentiating Cancer Immunotherapy Using Papaya Mosaic Virus-Derived Nanoparticles.

    Science.gov (United States)

    Lebel, Marie-Ève; Chartrand, Karine; Tarrab, Esther; Savard, Pierre; Leclerc, Denis; Lamarre, Alain

    2016-03-01

    The recent development of novel immunotherapies is revolutionizing cancer treatment. These include, for example, immune checkpoint blockade, immunomodulation, or therapeutic vaccination. Although effective on their own, combining multiple approaches will most likely be required in order to achieve the maximal therapeutic benefit. In this regard, the papaya mosaic virus nanoparticle (PapMV) has shown tremendous potential as (i) an immunostimulatory molecule, (ii) an adjuvant, and (iii) a vaccine platform through its intrinsic capacity to activate the innate immune response in an IFN-?-dependent manner. Here, we demonstrate that intratumor administration of PapMV significantly slows down melanoma progression and prolongs survival. This correlates with enhanced chemokine and pro-inflammatory-cytokine production in the tumor and increased immune-cell infiltration. Proportions of total and tumor-specific CD8(+) T cells dramatically increase following PapMV treatment whereas those of myeloid-derived suppressor cells (MDSC) concomitantly decrease. Moreover, systemic PapMV administration prevents metastatic tumor-implantation in the lungs. Importantly, PapMV also synergistically improves the therapeutic benefit of dendritic cell (DC)-based vaccination and PD-1 blockade by potentiating antitumor immune responses. This study illustrates the immunostimulatory potential of a plant virus-derived nanoparticle for cancer therapy either alone or in conjunction with other promising immunotherapies in clinical development. PMID:26891174

  2. CHARACTERIZATION OF PAPAYA FRUIT MEDIATED SILVER NANOPARTICLES AND EVALUATION OF ITS ANTIMICROBIAL AND WOUND HEALING ACTIVITY

    Directory of Open Access Journals (Sweden)

    EnamalaNarmadha

    2013-07-01

    Full Text Available The field of Nanotechnology is the most active area of research in modern material science. Though there are many chemical as well as physical methods, green synthesis of nanomaterial is the most emerging method of synthesis. We report the synthesis of antibacterial silver nanoparticles using Papaya. The synthesized AgNPs have been characterized by UV-Vis spectroscopy and SEM microscopy. The silver nanoparticles stabilized by Papaya fruit extract were found to have enhanced antimicrobial activity against well-known pathogenic strains Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus, Proteus vulgaris and E. coli.

  3. Color index and correlation with physical and chemical parameters of guava, mango and papaya

    OpenAIRE

    Jedman Dantas Motta; Alexandre José de Melo Queiroz; Rossana Maria Feitosa de Figueirêdo

    2015-01-01

    It was used a low cost colorimeter determine the color in agricultural products. Color analysis on peel and pulps of guava, papaya and mango were made. The values found in the L*a*b* color spaces were used to calculate color indexes that were related to the soluble solids content, pH and the fruit consistence during the maturation. The results obtained permit to conclude that it is possible to estimate the ripening of papaya over the color peel, having found an accurate relation between peel ...

  4. Genetic Diversity Based on Coat Protein of Papaya ringspot virus (Pathotype P) Isolates from Bangladesh

    OpenAIRE

    M. S. Akhter; Basavaraj, Y. B.; A. M. Akanda; Mandal, B; Jain, R. K.

    2013-01-01

    The coat protein (CP) sequences of twelve Papaya ringspot virus (PRSV) (pathotype-P) isolates from six major papaya growing areas were determined and compared with those of published PRSV. The CP coding region varied in size from 846–852 nucleotides, encoding a protein of 282–284 amino acids. Comparative CP sequence analysis revealed that the PRSV-P isolates originating from Bangladesh were divergent up to 14 % at amino acids level. Further, the isolates from Bangladesh shared 86–95 % amino a...

  5. Chemical characterization of the flour of peel and seed from two papaya cultivars

    OpenAIRE

    Cláudia Mendes dos Santos; Celeste Maria Patto Abreu; Juliana Mesquita Freire; Estela de Rezende Queiroz; Marcelle Mendes Mendonça

    2014-01-01

    Papaya is among the currently most important tropical fruits grown in Brazil and in the world. The fruit is mainly consumed fresh although it offers many industrial products. The processing of this fruit, as well as its fresh consumption, results in large amounts of waste, such as peels and seeds. Papaya consumption is one of the causes of significant loss of food value; therefore, new aspects on the use of its waste as by-products, or in the production of food additives, or even the incorpor...

  6. SIGNIFICADO DE LA HISTERECTOMÍA PARA UN GRUPO DE MUJERES CHILENAS

    Directory of Open Access Journals (Sweden)

    María Teresa Urrutia S

    2009-01-01

    Full Text Available Antecedentes: La histerectomía por patología benigna del útero, es una de las cirugías más frecuente después de la operación cesárea. Objetivo: Evaluar el significado que tiene la histerectomía para mujeres chilenas, beneficiarías de un hospital público de la Región Metropolitana de Santiago, Chile. Método: Estudio transversal descriptivo, de tipo cualitativo, sobre el significado que 120 mujeres histerectomizadas asigna a esa cirugía. Se describen 3 temas que reflejan el significado de la extracción del útero: pérdida, tristeza-dolor, y mejoría de una enfermedad. Resultados: El promedio de edad fue 56 ±11,9 años, y de escolaridad de 7,8 ± 4,3 años. El 63% de las mujeres fue histerectomizada por patología ginecológica benigna. El significado que la mujer atribuye a la extracción del útero se encuentra relacionado a una experiencia de pérdida, que si bien conlleva un alivio de la enfermedad, también implica dolor, tristeza y soledad. Conclusión: Sensibilizar a los profesionales de la salud, que trabajan con mujeres histerectomizadas, a comprender y respetar el significado que para ellas tiene la histerectomía y propiciar el ambiente adecuado para contenerlas y educarlas pre y pos cirugía.Background: Elective hysterectomy, after caesarian section, is one of the most frequent surgeries. Objective: To evaluate the significance that the hysterectomy has for Chilean women from a public hospital of the Metropolitan Region of Santiago, Chile. Method: Qualitative and cross-sectional design study of the significance of hysterectomy for 120 women. Three themes were derived from interviews: loss, sadness - sorrow, recovery from illness. Results: The mean of age was 56 ± 11.9 years; the educational level was 7.8 ± 4.3 years. A total of 63% of women had planned elective hysterectomy preformed. The significance that the women attribute to the hysterectomy is related to the experience of loss, and although it includes recovery from illness, it also signifies sorrow, sadness, and loneliness. Conclusion: The health care providers, who work with these women, need to be respectful and understanding of the significance that the women give to this surgery, and to give them the best setting for supporting and educating them before and after the surgery.

  7. Parámetros poblacionales de Tetranychus merganser Boudreaux (Acari: Tetranychidae) en papayo (Carica papaya L.) a diferentes temperaturas / Population parameters of Tetranychus merganser Boudreaux (Acari: Tetranychidae) in papaya (Carica papaya L.) at different temperatures

    Scientific Electronic Library Online (English)

    Noel, Reyes-Pérez; Juan A., Villanueva-Jiménez; Mónica, de la Cruz Vargas-Mendoza; Héctor, Cabrera-Mireles; Gabriel, Otero-Colina.

    2013-03-01

    Full Text Available El ácaro Tetranychus merganser es una de las especies que causa mayor daño económico en la zona productora de papayo (Carica papaya) del estado de Veracruz, México. Por tanto, se requiere conocer aspectos de su biología para tomar mejores decisiones de su manejo. Se obtuvo una colonia de ácaros a pa [...] rtir de una hembra recolectada en el municipio de Manlio F. Altamirano, y se incrementó la población en invernadero sobre hojas de frijol (Phaseolus vulgaris). El objetivo fue evaluar la influencia de la temperatura en los parámetros poblacionales de esta especie y sobre la duración de sus fases de desarrollo. Para ello se iniciaron cohortes desde huevecillos y expuestas a las siguientes condiciones: temperaturas constantes (19, 23, 27, 31, 33 y 35 °C); 60±2 % de humedad relativa; fotoperiodo de 14:10 h luz:oscuridad y alimentación en discos de hojas de papaya. La duración del ciclo de vida de T. merganser disminuyó de 52.3 hasta 12.9 d con el aumento de la temperatura. La tasa neta reproductiva (Ro) fue 37.40, 62.38, 43.98, 10.47 y 2.32 para 19, 23, 27, 31 y 33° C; a 35 °C la cohorte no fue viable. La tasa intrínseca de crecimiento poblacional (r m) fue 0.08, 0.19, 0.21, 0.18 y 0.12 a las temperaturas anotadas. El desarrollo óptimo ocurrió entre 23 y 27 °C. Aunque el papayo no es su mejor hospedero comparado con parámetros poblacionales más favorables obtenidos por otros autores en frijol, T. merganser puede comportarse como plaga en papayo en las condiciones ambientales predominantes en la zona. Abstract in english Tetranychus merganser is a mite species that causes greater economic damage in the papaya (Carica papaya) production area in the state of Veracruz, Mexico. Therefore, it was necessary to study their biology in order to make better decisions in their management. We obtained a colony of mites from a f [...] emale collected in the municipality of Manlio F. Altamirano, and expanded their population on bean leaves (Phaseolus vulgaris) under greenhouse conditions. The objective was to evaluate the influence of temperature on the population parameters of this species and the duration of their developmental stages. For this purpose, we established cohorts starting with eggs, and exposed them to the following conditions: constant temperatures (19, 23, 27, 31, 33 and 35 °C); 60±2 % relative humidity, photoperiod of 14:10 h light:dark, and fed with papaya leaf discs. The duration of the life cycle of T. merganser decreased from 52.3 to 12.9 d with the increase of temperature. The net reproductive rate (R) was 37.40, 62.38, 43.98, 10.47 and 2.32 for 19, 23, 27, 31 and 33 °C; at 35 °C, the cohort was not viable. The intrinsic rate of population increase (r m) was 0.08, 0.19, 0.21, 0.18 and 0.12 at the temperatures recorded. Optimal development occurred between 23 and 27 C. Although papaya is not its best host compared to more favorable population parameters obtained by other authors in beans, T. merganser can behave like a pest in papaya under the environmental conditions prevailing in the area.

  8. Low-dose gamma irradiation following hot water immersion of papaya (Carica papaya linn.) fruits provides additional control of postharvest fungal infection to extend shelf life

    Science.gov (United States)

    Rashid, M. H. A.; Grout, B. W. W.; Continella, A.; Mahmud, T. M. M.

    2015-05-01

    Low-dose gamma irradiation (0.08 kGy over 10 min), a level significantly below that required to satisfy the majority of international quarantine regulations, has been employed to provide a significant reduction in visible fungal infection on papaya fruit surfaces. This is appropriate for local and national markets in producer countries where levels of commercial acceptability can be retained despite surface lesions due to fungal infection. Irradiation alone and in combination with hot-water immersion (50 °C for 10 min) has been applied to papaya (Carica papaya L.) fruits at both the mature green and 1/3 yellow stages of maturity. The incidence and severity of surface fungal infections, including anthracnose, were significantly reduced by the combined treatment compared to irradiation or hot water treatment alone, extending storage at 11 °C by 13 days and retaining commercial acceptability. The combined treatment had no significant, negative impact on ripening, with quality characteristics such as surface and internal colour change, firmness, soluble solids, acidity and vitamin C maintained at acceptable levels.

  9. Estudio de la conservación de la papaya (Carica papaya L. asociado a la aplicación de películas comestibles

    Directory of Open Access Journals (Sweden)

    Alessandra Almeida Castro

    2011-06-01

    Full Text Available Brasil es un importante productor de papaya, pero sólo una pequeña parte se exporta. Esta fruta es altamente perecedera, por lo que el control de la maduración es esencial para aumentar la vida útil, tanto para el mercado interno como para la exportación. Una alternativa que ha cobrado impulso en el mercado es el uso de películas comestibles biodegradables, entre ellas la película producida a partir de almidón de yuca. En este estudio se evaluó el efecto de una película de almidón de yuca (2 % sobre la papaya almacenadas a temperatura ambiental (25 ± 2 ºC y a 8 °C, y 82 % de humedad relativa durante 6 días de almacenamiento. Se realizaron análisis de pérdida de peso, color, pH, acidez, carotenoides totales, vitamina C y sólidos solubles totales, cada tres días de almacenamiento. La película comestible de almidón de yuca resultó ser una buena alternativa para la preservación de la papaya durante seis días de almacenamiento, en relación a los parámetros pH, grados Brix, acidez y carotenoides.

  10. Estudio de la conservación de la papaya (Carica papaya L. asociado a la aplicación de películas comestibles

    Directory of Open Access Journals (Sweden)

    Alessandra Almeida Castro

    2011-01-01

    Full Text Available Brasil es un importante productor de papaya, pero sólo una pequeña parte se exporta. Esta fruta es altamente perecedera, por lo que el control de la maduración es esencial para aumentar la vida útil, tanto para el mercado interno como para la exportación. Una alternativa que ha cobrado impulso en el mercado es el uso de películas comestibles biodegradables, entre ellas la película producida a partir de almidón de yuca. En este estudio se evaluó el efecto de una película de almidón de yuca (2 % sobre la papaya almacenadas a temperatura ambiental (25 ± 2 ºC y a 8 °C, y 82 % de humedad relativa durante 6 días de almacenamiento. Se realizaron análisis de pérdida de peso, color, pH, acidez, carotenoides totales, vitamina C y sólidos solubles totales, cada tres días de almacenamiento. La película comestible de almidón de yuca resultó ser una buena alternativa para la preservación de la papaya durante seis días de almacenamiento, en relación a los parámetros pH, grados Brix, acidez y carotenoides.

  11. Purification of papain from fresh latex of Carica papaya

    Directory of Open Access Journals (Sweden)

    Rubens Monti

    2000-01-01

    Full Text Available In the present study we wish to report a method of crystallizing papain from fresh papaya latex which gave higher yields than previously reported. This method does not involve the use of sulphydryl reagents. The papain thus obtained is practically pure and shows a single band when submitted to electrophoresis on polyacrylamide gel, and is identical to the papain obtained by other methods. In routine enzymatic assays, specific activity was measured using Z-gly-pNP and BAEE as substrates. Papain crystallized by this method, without the use of high concentrations of salts or thiol-containing substances such as cysteine and dithiothreitol, is obtained in the form of a complex with natural inhibitors existent in latex which can be removed by dialysis.No presente trabalho apresenta-se um método de cristalização da papaína oriunda do látex fresco de mamão, o qual apresenta uma alta produtividade em relação aos métodos previamente descritos. A metodologia aqui descrita não envolve o uso de reagentes sulfidrílicos, a papaína foi obtida de forma praticamente pura, apresentando uma simples banda quando submetida a eletroforese, e com propriedades idênticas àquelas obtidas por outros métodos. A atividade específica foi determinada utilizando Z-gly-pNP e BAEE como substrato. A papaína obtida por essa metodologia, sem uso de substâncias tais como cisteína e ditiotreitol, apresenta-se na forma de um complexo com inibidores naturais, os quais podem ser removidos por diálise.

  12. Engineered Antibody Fragments for Immunodiagnosis of Papaya ringspot virus.

    Science.gov (United States)

    Maheshwari, Yogita; Verma, H N; Jain, R K; Mandal, Bikash

    2015-07-01

    The present study was undertaken to clone and express the genes encoding antibody to the recombinant coat protein (rCP) of Papaya ringspot virus (PRSV) and to assess the engineered antibody for the detection of PRSV. A 33-kDa rCP of PRSV, which was produced in Escherichia coli, generated PRSV specific antibody in immunized mouse. The heavy and light chain variable domain genes (VH and VL) of 351 and 360 nucleotides, respectively, were cloned from the mRNA isolated from the spleen of the immunized mouse with rCP of PRSV. The VH and VL belong to the family IgG1 and kappa chain, respectively, and contained the framework regions and complementarity determining regions. The VH and VL genes were individually used to develop the expression constructs in pET28a (+) vector and 14-kDa proteins were obtained in E. coli. The amount of purified VH and VL proteins was 3-4 mg/l of bacterial culture. Both the antibody fragments recognized PRSV in the crude sap; however, the VL antibody fragment showed higher affinity to PRSV. The mixture of VH and VL detected PRSV as effectively as polyclonal antibody. The recombinant antibody fragments mixture detected PRSV in the field samples with 100 % accuracy in dot immunobinding assay (DIBA) and enzyme-linked immunosorbent assay (ELISA). The sensitivity of the detection of PRSV using antibody fragments was 1.0 and 10.0 ng in DIBA and ELISA, respectively. The results showed successful isolation of functional single-domain antibody encoding genes to PRSV directly from the immunized spleen cells of mouse. This study for the first time demonstrates application of bacterial expressed recombinant antibody fragments in immunodiagnosis of PRSV. PMID:25854961

  13. Thrombocyte counts in mice after the administration of papaya leaf suspension.

    Science.gov (United States)

    Sathasivam, Kathiresan; Ramanathan, Surash; Mansor, Sharif M; Haris, Mas Rosemal M H; Wernsdorfer, Walther H

    2009-10-01

    Following up a popular use of crude leaf preparations from Carica papaya for the treatment of dengue infections, a suspension of powdered Carica papaya leaves in palm oil has been investigated for its effect on thrombocyte counts in mice, administering by gavage 15 mg of powdered leaves per kg body weight to 5 mice. Equal numbers of animals received corresponding volumes of either palm oil alone or physiological saline solution. Thrombocyte counts before and at 1, 2, 4, 8, 10, 12, 24, 48 and 72 hours after dosing revealed significantly higher mean counts at 1, 2, 4, 8, 10 and 12 after dosing with the C. papaya leaf formulation as compared to the mean count at hour 0. There was only a non-significant rise of thrombocyte counts in the group having received saline solution, possibly the expression of a normal circadian rhythm in mice. The group having received palm oil only showed a protracted increase of platelet counts that was significant at hours 8 and 48 and obviously the result of a hitherto unknown stimulation of thrombocyte release. The results call for a dose-response investigation and for extending the studies to the isolation and identification of the C. papaya substances responsible for the release and/or production of thrombocytes. PMID:19915811

  14. How to become a tree without wood - biomechanical analysis of the stem of Carica papaya L.

    Science.gov (United States)

    Kempe, A; Lautenschläger, T; Lange, A; Neinhuis, C

    2014-01-01

    Carica papaya L. does not contain wood, according to the botanical definition of wood as lignified secondary xylem. Despite its parenchymatous secondary xylem, these plants are able to grow up to 10-m high. This is surprising, as wooden structural elements are the ubiquitous strategy for supporting height growth in plants. Proposed possible alternative principles to explain the compensation for lack of wood in C. papaya are turgor pressure of the parenchyma, lignified phloem fibres in the bark, or a combination of the two. Interestingly, lignified tissue comprises only 5-8% of the entire stem mass. Furthermore, the phloem fibres do not form a compact tube enclosing the xylem, but instead form a mesh tubular structure. To investigate the mechanism of papaya's unusually high mechanical strength, a set of mechanical measurements were undertaken on whole stems and tissue sections of secondary phloem and xylem. The structural Young's modulus of mature stems reached 2.5 GPa. Since this is low compared to woody plants, the flexural rigidity of papaya stem construction may mainly be based on a higher second moment of inertia. Additionally, stem turgor pressure was determined indirectly by immersing specimens in sucrose solutions of different osmolalities, followed by mechanical tests; turgor pressure was between 0.82 and 1.25 MPa, indicating that turgor is essential for flexural rigidity of the entire stem. PMID:23656471

  15. Chemical Characterization and in Vitro Cytotoxicity on Squamous Cell Carcinoma Cells of Carica Papaya Leaf Extracts

    Science.gov (United States)

    Nguyen, Thao T.; Parat, Marie-Odile; Hodson, Mark P.; Pan, Jenny; Shaw, Paul N.; Hewavitharana, Amitha K.

    2015-01-01

    In traditional medicine, Carica papaya leaf has been used for a wide range of therapeutic applications including skin diseases and cancer. In this study, we investigated the in vitro cytotoxicity of aqueous and ethanolic extracts of Carica papaya leaves on the human oral squamous cell carcinoma SCC25 cell line in parallel with non-cancerous human keratinocyte HaCaT cells. Two out of four extracts showed a significantly selective effect towards the cancer cells and were found to contain high levels of phenolic and flavonoid compounds. The chromatographic and mass spectrometric profiles of the extracts obtained with Ultra High Performance Liquid Chromatography-Quadrupole Time of Flight-Mass Spectrometry were used to tentatively identify the bioactive compounds using comparative analysis. The principal compounds identified were flavonoids or flavonoid glycosides, particularly compounds from the kaempferol and quercetin families, of which several have previously been reported to possess anticancer activities. These results confirm that papaya leaf is a potential source of anticancer compounds and warrant further scientific investigation to validate the traditional use of papaya leaf to treat cancer. PMID:26712788

  16. Induction of Resistance to Papaya Black Spot Elicited by Acibenzolar-S-Methyl

    Directory of Open Access Journals (Sweden)

    A.A.R. Oliveira

    2014-01-01

    Full Text Available The objective of this study was to evaluate the effect of acibenzolar-S-methyl tested at 5 concentrations (0, 1, 5, 25 and 100 ?M a.i. for its ability to protect papaya (Carica papaya cv. Rainbow from black spot (Asperisporium caricae following inoculation with the fungus. Effects of resistance induction treatment against black spot disease were evaluated by measuring the plant height and stem diameter. Disease symptoms were scored weekly by visually estimating disease severity of plants on the basis of a 5-class visual scale of 0 (no symptoms to 4 (extensive lesions on leaves. Accumulation of defence-related proteins in papaya leaves were also analysed and compared. Results revealed that the level of protection against A. caricae was dose-dependent. Maximum reduction of the disease in leaves was obtained with 25-100 ?M acibenzolar-S-methyl, with a time interval of 3 days between application of the activator and inoculation with the pathogen. The systemic resistance elicitation was characterized by an increase in 2 pathogenesis-related proteins, chitinase and ?-1, 3-glucanase. These results indicate that acibenzolar-S-methyl induces partial resistance in papaya against black spot disease which may provide the grower a new option for integrated management of the disease.

  17. Chemical Characterization and in Vitro Cytotoxicity on Squamous Cell Carcinoma Cells of Carica Papaya Leaf Extracts.

    Science.gov (United States)

    Nguyen, Thao T; Parat, Marie-Odile; Hodson, Mark P; Pan, Jenny; Shaw, Paul N; Hewavitharana, Amitha K

    2015-01-01

    In traditional medicine, Carica papaya leaf has been used for a wide range of therapeutic applications including skin diseases and cancer. In this study, we investigated the in vitro cytotoxicity of aqueous and ethanolic extracts of Carica papaya leaves on the human oral squamous cell carcinoma SCC25 cell line in parallel with non-cancerous human keratinocyte HaCaT cells. Two out of four extracts showed a significantly selective effect towards the cancer cells and were found to contain high levels of phenolic and flavonoid compounds. The chromatographic and mass spectrometric profiles of the extracts obtained with Ultra High Performance Liquid Chromatography-Quadrupole Time of Flight-Mass Spectrometry were used to tentatively identify the bioactive compounds using comparative analysis. The principal compounds identified were flavonoids or flavonoid glycosides, particularly compounds from the kaempferol and quercetin families, of which several have previously been reported to possess anticancer activities. These results confirm that papaya leaf is a potential source of anticancer compounds and warrant further scientific investigation to validate the traditional use of papaya leaf to treat cancer. PMID:26712788

  18. Anthelmintic activity of papaya seeds on Hymenolepis diminuta infections in rats.

    Science.gov (United States)

    Sapaat, A; Satrija, F; Mahsol, H H; Ahmad, A H

    2012-12-01

    The purpose of this study is to see the anthelmintic activity potential of papaya seeds against Hymenolepis diminuta in rats. The objectives of this study were: (1) to determine the effectiveness of papaya seeds on helminths especially H. diminuta in rats and (2) to determine the effective dose level on helminths in rats. Thirty six male rats from strain Sprague-Dawley were chosen as samples in this experiment. Two types of dose level were used for papaya seeds treatments such as 0.6 g kg-1 and 1.2 g kg-1. The geometric mean (GEM) was used to calculate mean for eggs per gram (EPG) before and after the treatment to be included in the reduction percentage calculation. After 21 days post treatment, necropsies were done to get the worm count and the GEM was used to calculate the efficacy percentage for the treatment. Results from this study showed that the reduction percentages in EPG for papaya seeds treatment for both doses level were very high which is 96.8% for 0.6g kg-1 dose level and 96.2% for 1.2 g kg-1 dose level. Whereas the efficacy percentage based on the worm counts for both doses level were also very high that was 90.77% for 0.6 g kg-1 dose level and 93.85% for 1.2 g kg-1. PMID:23202594

  19. Biodiesel Production by Enzymatic Transesterification of Papaya Seed Oil and Rambutan Seed Oil

    Directory of Open Access Journals (Sweden)

    C. S. Wong

    2014-12-01

    Full Text Available Biodiesel production from vegetable oil has gained attention as an alternative fuel to minimize the usage of fossil fuels and reduce greenhouse gases pollution. In Malaysia, oils from local fruit seeds of papaya and rambutan are potential feedstock for biodiesel production due to their high lipid contents and easily available. In the present study, papaya and rambutan seed oils were extracted via soxhlet apparatus using n-hexane and the oil yields were in between 34–40%. The extracted oils were subjected to enzymatic transesterification by the immobilized Candida rugosa lipase as a catalyst under room temperature with varies molar ratios of methanol to oil. The highest biodiesel yield for papaya seed oil and rambutan seed oil was found to be 96% and 89% at methanol-to-oil ratios of 6:1 and 8:1, respectively. Results also showed a higher biodiesel yield using lipase immobilized on the magnetic particles as the heterogeneous catalyst compared to the yield obtained using free enzyme as the homogeneous catalyst. The properties of biodiesel such as density, acid value, iodine value and cetane number were analyzed and found to meet the European Standard of Biodiesel. The study shows that papaya and rambutan seed oils have the potential to be used as alternative feedstock for biodiesel production than the full dependence on palm oil in Malaysia.

  20. Polyethylene glycol effects on somatic embryogenesis of papaya hybrid UENF/CALIMAN 01 seeds

    Scientific Electronic Library Online (English)

    Angelo Schuabb, Heringer; Ellen Moura, Vale; Tatiana, Barroso; Claudete, Santa-Catarina; Vanildo, Silveira.

    Full Text Available Among commercial fruits, papaya (Carica papaya L.) is notably one of the most common ones around the world. The aim of the current study was to establish a somatic embryogenesis protocol for the hybrid UENF/CALIMAN 01, elucidating the effects of sucrose and 2,4-dichlorophenoxyacetic acid (2,4-D) on [...] the induction of embryogenic cultures and the effect of polyethylene glycol (PEG) on somatic embryo maturation. Immature zygotic embryos of C. papaya hybrid UENF/CALIMAN 01 were cultured in MS culture medium supplemented with different concentrations of sucrose (30 and 60 g L-1) and 2,4-D (0, 20, 40 and 80 ?M). The combination of 30 g L-1 sucrose and 20 ?M 2,4-D resulted in the highest induction rates and the largest callus diameters. Furthermore, this combination was associated with the greatest potential to form somatic embryos. To promote maturation, cultures were inoculated in MS medium and exposed to different concentrations of PEG (0, 30 and 60 g L-1). The 60 g L-1 PEG treatment group had the highest average for the number, maturation and conversion of somatic embryos that resulted in plantlets at the end of the process. The addition of PEG to MS medium led to increased protein synthesis. These results are unprecedented for C. papaya hybrid UENF/CALIMAN 01 as the current study demonstrates the development of somatic embryogenesis in this high quality hybrid.

  1. Chemical treatment of papaya seeds aiming at long-term storage and control of damping off

    Directory of Open Access Journals (Sweden)

    Silvia de Carvalho Campos Botelho

    2014-06-01

    Full Text Available Damping off is a nursery disease of great economic importance in papaya and seed treatment may be an effective measure to control. The aim of this work was to evaluate the quality of papaya seeds treated with fungicides and stored under two environmental and packaging conditions. Additionally, the efficiency of fungicide treatments in the control of damping-off caused by Rhizoctonia solani was evaluated. Papaya seeds were treated with the fungicides Captan, Tolylfluanid and the mixture Tolylfluanid + Captan (all commercial wettable powder formulations. Seeds of the control group were not treated. The seeds were stored for nine months in two conditions: packed in aluminum coated paper and kept at 7 ± 1ºC and in permeable kraft paper and kept in non-controlled environment. At the beginning of the storage and every three months the seed quality (germination and vigor tests, emergence rate index, height, dry mass and damping of plants in pre and post-emergence (in contaminated substrate and mycelia-free substrate were analyzed. Both storage conditions as well as the fungicide treatments preserved the germination and seed vigor. In the infested substrate, seedling emergence was favored by fungicides, but in post-emergence, fungicides alone did not control the damping off caused by R. solani. Symptoms of damping off were not observed in the clean substrate. The results showed that the fungicide treatments may be used to pretreat papaya seed for long-term storage and commercialization.

  2. Assessment of the Anti-Protozoal Activity of Crude Carica papaya Seed Extract against Trypanosoma cruzi

    Directory of Open Access Journals (Sweden)

    Karla Y. Acosta-Viana

    2013-10-01

    Full Text Available In order to determine the in vivo activity against the protozoan Trypanosoma cruzi, two doses (50 and 75 mg/kg of a chloroform extract of Carica papaya seeds were evaluated compared with a control group of allopurinol. The activity of a mixture of the three main compounds (oleic, palmitic and stearic acids in a proportion of 45.9% of oleic acid, 24.1% of palmitic and 8.52% of stearic acid previously identified in the crude extract of C. papaya was evaluated at doses of 100, 200 and 300 mg/kg. Both doses of the extracts were orally administered for 28 days. A significant reduction (p < 0.05 in the number of blood trypomastigotes was observed in animals treated with the evaluated doses of the C. papaya extract in comparison with the positive control group (allopurinol 8.5 mg/kg. Parasitemia in animals treated with the fatty acids mixture was also significantly reduced (p < 0.05, compared to negative control animals. These results demonstrate that the fatty acids identified in the seed extracts of C. papaya (from ripe fruit are able to reduce the number of parasites from both parasite stages, blood trypomastigote and amastigote (intracellular stage.

  3. Chemical Characterization and in Vitro Cytotoxicity on Squamous Cell Carcinoma Cells of Carica Papaya Leaf Extracts

    Directory of Open Access Journals (Sweden)

    Thao T. Nguyen

    2015-12-01

    Full Text Available In traditional medicine, Carica papaya leaf has been used for a wide range of therapeutic applications including skin diseases and cancer. In this study, we investigated the in vitro cytotoxicity of aqueous and ethanolic extracts of Carica papaya leaves on the human oral squamous cell carcinoma SCC25 cell line in parallel with non-cancerous human keratinocyte HaCaT cells. Two out of four extracts showed a significantly selective effect towards the cancer cells and were found to contain high levels of phenolic and flavonoid compounds. The chromatographic and mass spectrometric profiles of the extracts obtained with Ultra High Performance Liquid Chromatography-Quadrupole Time of Flight-Mass Spectrometry were used to tentatively identify the bioactive compounds using comparative analysis. The principal compounds identified were flavonoids or flavonoid glycosides, particularly compounds from the kaempferol and quercetin families, of which several have previously been reported to possess anticancer activities. These results confirm that papaya leaf is a potential source of anticancer compounds and warrant further scientific investigation to validate the traditional use of papaya leaf to treat cancer.

  4. Application of Transgenic Technologies to Papaya: Developments and Biosafety Assessments in Thailand.

    Czech Academy of Sciences Publication Activity Database

    Kertbundit, Sunee; Ju?í?ek, Miloslav

    2010-01-01

    Ro?. 4, ?. 1 (2010), s. 52-57. ISSN 1749-0413 Institutional research plan: CEZ:AV0Z50380511 Keywords : coat protein-mediated resistance * GMO * Papaya ringspot virus Subject RIV: EF - Botanics http://home.ueb.cas.cz/publikace/2010_Kertbundit_TransgenicPlantJournal_52.pdf

  5. Chemical characterization of the flour of peel and seed from two papaya cultivars

    Directory of Open Access Journals (Sweden)

    Cláudia Mendes dos Santos

    2014-06-01

    Full Text Available Papaya is among the currently most important tropical fruits grown in Brazil and in the world. The fruit is mainly consumed fresh although it offers many industrial products. The processing of this fruit, as well as its fresh consumption, results in large amounts of waste, such as peels and seeds. Papaya consumption is one of the causes of significant loss of food value; therefore, new aspects on the use of its waste as by-products, or in the production of food additives, or even the incorporation of its flour in food have aroused great interest because these are products of high nutritional value, and their use may be economically viable. The objective of this study was to produce and characterize peel and seed flours from two papaya cultivars (Havai and Calimosa for their chemical constituents focusing on possible use in foods. The proximate and mineral composition, titratable acidity, soluble solids, pH, contents of vitamin C, and phenolic compounds were determined. According to the results obtained, the papaya peel and seed flours had high contents of protein and fiber and therefore can be used as alternative sources of nutrients and can also be added in foods avoiding waste and adding value to the fruit.

  6. Patrones de crecimiento urbano en la Patagonia chilena: el caso de la ciudad de Coyhaique

    Directory of Open Access Journals (Sweden)

    Gerardo Azócar García

    2010-01-01

    Full Text Available Las políticas de Estado junto con la importante oferta/demanda de productos inmobiliarios para población de mayor ingreso han orientado el desarrollo reciente de varias ciudades chilenas, originando nuevas formas urbanas. Si bien estos procesos han sido descritos y analizados para varias ciudades y áreas metropolitanas de Chile central, no existen suficientes antecedentes empíricos que den cuenta de la realidad del desarrollo urbano de las ciudades más australes del país, como el caso de Coyhaique en la Patagonia chilena. Este trabajo pretende identificar y analizar los patrones de urbanización de esta ciudad, en un contexto territorial caracterizado, entre otros aspectos, por la fragmentación geográfica, el aislamiento, dispersión de los centros poblados y concentración de la población en la capital regional. Se analiza la evolución y factores explicativos que dan cuenta de su rápido proceso de urbanización y, sobre la base de documentos técnicos, históricos y geográficos, se presentan estimaciones futuras del crecimiento de la ciudad.

  7. Propiedades Psicométricas del Inventario de Orientación Temporal de Zimbardo en una muestra Chilena

    Directory of Open Access Journals (Sweden)

    Cristián Oyanadel

    2014-01-01

    Full Text Available La percepción del tiempo es una capacidad esencial para la adaptación del ser humano al ambiente. Un as - pecto de su estudio, la orientación temporal, caracteriza la actitud de las personas hacia el presente, pasado y futuro. e l Z tp I ha sido adaptado en múltiples países, y se han descrito aplicaciones en psicología clínica y de la salud. Este estudio busca adaptar y describir las propiedades del instrumento en una muestra chilena. Se reclutaron 604 participantes, entre 18 y 70 años. Las propiedades de los ítems y el análisis factorial explora - torio, señalan que es posible mantener la estructura original del instrumento con cinco factores. Reubicando 6 ítems el Alpha de Cronbach va desde .59 para Pasado Positivo a .80 para Pasado Negativo y Futuro. Son discutidas diferencias de género y edad. Siendo necesario otros estudios de validación, se establece como test útil para investigar la orientación temporal en población chilena.

  8. Influencia del tamaño empresarial sobre los resultados: un estudio comparativo entre empresas chilenas y españolas

    Directory of Open Access Journals (Sweden)

    Patricia Huerta

    2010-01-01

    Full Text Available Con la finalidad de contribuir en la importancia que tiene el tamaño empresarial en el resultado que alcanzan las organizaciones, la presente investigación persigue dos objetivos. En primer lugar, realizar una revisión bibliográfica del tamaño de las empresas y su relación con los resultados empresariales, y en segundo lugar, contrastar los planteamientos anteriores para aportar evidencia empírica al respecto, a través de un análisis comparativo entre empresas chilenas y españolas. El contraste se realiza sobre un panel completo de empresas industriales chilenas y españolas, para el periodo 1999-2005 y se emplea un modelo de efectos aleatorios. Utilizando técnicas para datos de panel se descubre que existe una relación entre el tamaño y los resultados empresariales, y además que el tamaño es una variable que influye significativamente en los resultados de las empresas. En conclusión, las empresas que deseen alcanzar mejores resultados deben considerar la dimensión empresarial o su tamaño como una variable relevante para dicho propósito.

  9. Las relaciones civiles-militares en el marco de la participación chilena en operaciones de paz

    Directory of Open Access Journals (Sweden)

    Jorge Riquelme Rivera

    2012-01-01

    Full Text Available El trabajo analiza las relaciones entre civiles y militares en el contexto de la participación chilena en operaciones de paz, concentrándose especialmente en la Misión de Estabilización de Naciones Unidas en Haití (MINUSTAH, establecida el año 2004. En tal sentido -y considerando la creciente convergencia entre la política exterior de Chile y su política de defensa-, se sostiene que la participación chilena en operaciones de paz ha contribuido a la integración entre civiles y militares, por cuanto ha favorecido una estrategia de trabajo conjunta e integradora entre el Ministerio de Relaciones Exteriores, el Ministerio de Defensa y las fuerzas armadas; así como un estrecho y coordinado trabajo entre los variados actores civiles y militares que laboran en el territorio haitiano.

  10. Effect of Temperature on the Development and Survival of Immature Stages of the Carambola Fruit Fly, Bactrocera carambolae, and the Asian Papaya Fruit Fly, Bactrocera papayae, Reared On Guava Diet

    OpenAIRE

    Danjuma, Solomon; Thaochan, Narit; Permkam, Surakrai; Satasook,Chutamas

    2014-01-01

    Members of the Bactrocera dorsalis Hendel (Diptera: Tephritidae) complex constitute wellrecognized destructive pests of fruits in peninsular Thailand. The development and survival of immature stages of the carambola fruit fly, Bactrocera carambolae Drew & Hancock, and the Asian papaya fruit fly, Bactrocera papayae Drew & Hancock, were compared at six constant temperatures of 15, 20, 25, 27, 30, and 35°C, 70 ± 5% relative humidity, and a photoperiod of 12:12 (L:D). The objectives were to deter...

  11. Efficacy of Essential Oils on the Conidial Germination, Growth of Colletotrichum gloeosporioides (Penz.) Penz. and Sacc and Control of Postharvest Diseases in Papaya (Carica papaya L.)

    OpenAIRE

    Laura Leticia Barrera-Necha; Silvia Bautista-Banos; Hilda Elizabeth Flores-Moctezuma; Abel Rojas Estudillo

    2008-01-01

    The efficacy of nine essential oils were investigated in vitro on conidial germination and mycelial growth inhibition of Colletotrichum gloeosporioides isolate from papaya (Carica papaya L.). In general, better antifungal effect was observed with Cinnamomum zeylanicum and Syzygium aromaticum oils which had strong inhibition of conidial germination of C. gloeosporioides at 50, 100, 150, 200 and 250 ?g mL-1 and a dose dependent inhibition mycelial growth was caused by these oils. Teloxys...

  12. Green synthesis and antibacterial activity screening of silver nanoparticles reduced by papaya (Carica papaya L.) leaves extract

    International Nuclear Information System (INIS)

    The field of nano technology is the most active area of research in modern material sciences. Though there are many chemical, as well as physical methods, green synthesis is the most emerging method of synthesis. This study aimed to describe a cost effective and environment friendly technique for green synthesis of silver nanoparticles. The synthesis of silver nanoparticles was prepared by adding Carica papaya L. leaves extract to 1mM silver nitrate solution. The color change in reaction mixture (pale yellow to dark brown color was observed during the incubation period , due to excitation of surface plasmon vibrations in silver nanoparticles. Nanoparticles were characterized using UV-Visible absorption spectroscopy, X-Ray Diffraction (XRD) pattern, Scanning Electron Microscopy (SEM) and Energy-Dispersive Spectroscopy (EDX) analysis. Absorption spectra of silver nanoparticles formed in the reaction media has absorbance peak at 280 nm, broadening of peak indicates that the particles are poly dispersed. SEM analysis described the morphology and the size of the particles. XRD confirmed the crystalline structure of the nanoparticles. The presence of the elemental silver was observed in the graph obtained from EDX analysis, which also supports the XRD results. The biomass of plants produces their nano materials by a process called bio mineralization. The tests cultures included in the study were Staphylococcus aureus, Escherichia coli and Salmonella. Results showed that the maximum inhibitory effect using 1mM silver nitrates against the microbes were obtained. The approach of plant-mediated synthesis appears to be cost efficient, eco-friendly and easy alternative to conventional methods of silver nanoparticles synthesis (author)

  13. Evaluation of the Combination of Vitamin D3 and Papaya Leaf on Muscle Antioxidant Activity of Spent Chicken

    Directory of Open Access Journals (Sweden)

    A.Q. Sazili

    2011-01-01

    Full Text Available Eighty spent chickens were employed in this study to assay the effect of combination of vitamin D3 and papaya leaf on antioxidant activity of meat in spent layer hens. Diets were a corn-soybean meal based diet for finisher layer with and without vitamin D3 which was supplemented with different levels of 0, 0.5, 1 and 2% for papaya leaf meal. Experiment lasted for 21 days. At day 0, 7, 14 and 21, the birds were scarified and breast muscle was obtained to determine antioxidant activity. Antioxidant activity was measured using 1, 1-Diphenyl-2-Picrylhydrazyl (DPPH. Result obtained from this study demonstrated that antioxidant activity of meat showed remarkable improvement between dietary treatments fed mix of vitamin D3 and papaya leaf and control group. In conclusion, vitamin D3 and papaya leaf when combined indicated an improvement in antioxidant activity of the spent meat.

  14. 76 FR 49725 - Notice of Decision To Authorize the Importation of Fresh Papaya Fruit From Malaysia into the...

    Science.gov (United States)

    2011-08-11

    ...fruit (Carica papaya) from Malaysia. The pest risk analysis consisted of a pest...significance that are present in Malaysia and could follow the pathway...into the United States and a risk management document (RMD)...

  15. Perfil Antropométrico de Jugadoras Chilenas de Fútbol Femenino Anthropometric Profile of Female Football - Soccer Chilean Players

    OpenAIRE

    Atilio Aldo Almagiá Flores; Fernando Rodríguez Rodríguez; Fernando Omar Barrraza Gómez; Pablo José Lizana Arce; Carlos Alberto Jorquera Aguilera

    2008-01-01

    Se evaluó un total de 43 jugadoras de fútbol, 26 seleccionadas chilenas sub 20 y 17 jugadoras del plantel campeón de fútbol universitario 2007 de la Pontificia Universidad Católica de Valparaíso (PUCV), todas ellas bajo su consentimiento informado. Se evaluó utilizando el protocolo de mareaje y medición de la International Society for the Avancement in Kineanthropometric (IS AK), en condiciones normales de temperatura, a primera hora de la mañana y después del vaciado urinario, por evaluadore...

  16. DISCURSO ACADÉMICO DE LANZAMIENTO DE LA REVISTA CHILENA DE DERECHO Y CIENCIA POLÍTICA

    Directory of Open Access Journals (Sweden)

    Patricio - Ignacio Carvajal

    2012-07-01

    Full Text Available Me complace enormemente la invitación que se me ha cursado para dirigir algunaspalabras en el lanzamiento de esta Revista Chilena de Derecho y Ciencia Política.Me honra el hecho de que me hayan tenido Uds. en consideración para este actoacadémico y, por supuesto, también que me hayan incorporado al elenco de especialistasde su Comité Científi co Externo. Por ello, comienzo por expresar mi sinceroagradecimiento.

  17. Prevalencia de vaginosis bacteriana en trabajadoras sexuales chilenas Prevalence of bacterial vaginosis in Chilean sex workers

    OpenAIRE

    Gloria Venegas; Gioconda Boggiano; Erica Castro

    2011-01-01

    OBJETIVO: Determinar la prevalencia de vaginosis bacteriana (VB) en trabajadoras sexuales chilenas y relacionar los hallazgos con variables sociodemográficas, sexuales y clínicas. MÉTODO: Se estudió una muestra de 379 trabajadoras sexuales que asistían para control a Unidades de Atención y Control de Salud Sexual de Chile. A todas se las entrevistó para obtener antecedentes sociodemográficos y sexuales, se les realizó evaluación clínica que incluyó características del flujo vaginal, pH y prue...

  18. Efecto del cloruro de sodio sobre el crecimiento y estado nutricional de plantas de papaya / Effect of sodium chloride on growth and mineral content of papaya plant

    Scientific Electronic Library Online (English)

    Jorge, Parés; Carmen, Basso.

    2013-08-01

    Full Text Available La limitada disponibilidad de agua de calidad para la agricultura de las zonas áridas y semiáridas ha propiciado el uso de agua de moderada a alta salinidad. La papaya (Carica papaya L.), aunque se reporta con diferentes sensibilidades al estrés salino, es una especie de gran potencial frutícola par [...] a ser cultivada económicamente en las regiones semiáridas del estado Lara, Venezuela. El objetivo de este ensayo fue evaluar el efecto de la salinidad ocasionada por el NaCl sobre el crecimiento vegetativo y el estado nutricional de la papaya ‘Maradol’ desarrollada bajo una estructura de techo transparente. Como fuente salina se utilizó cloruro de sodio grado analítico, el cual se adicionó a través del agua de riego en cuatro tratamientos (0,001; 2,0; 4,0 y 8,0 dS·m-1) bajo un diseño completamente aleatorizado con cuatro repeticiones, hasta finalizar la fase juvenil del desarrollo. Se evaluó la longitud del tallo, biomasa seca aérea y radical, área foliar, área de sección del tallo, contenido relativo de clorofila (SPAD) y el contenido de N, P, K, Ca, Mg, Mn, Zn, Cu, Fe y Na a nivel foliar. Los tratamientos afectaron el crecimiento vegetativo de las plantas durante toda la fase juvenil del desarrollo. Se observó, además, reducción del contenido de clorofila con el estrés por NaCl. Los tenores N y Na incrementaron, mientras que, los de K, Zn, Cu y Fe disminuyeron. Los niveles de P, Ca, Mg y Mn, no se afectaron. En base a los resultados obtenidos la papaya ‘Maradol’ puede ser considerada como un cultivar de moderada tolerancia al NaCl. Abstract in english Due to limited availability of low-salinity water in arid and semi-arid regions, water use of moderate to high salinity in agriculture of those regions is unavoidable. Papaya, although exhibits different sensitivities to salinity, has great potential to be cultivated in semi-arid regions of Lara Sta [...] te, Venezuela. The objective of this research was to evaluate the effects of irrigation with water containing different levels of NaCl on growth and nutrient content of papaya ‘Maradol’. The experiment was carried out under a structure with transparent ceiling until the end of the plant juvenile phase. NaCl treatments were applied to irrigation water to obtain 0.001, 2.0, 4.0 and 8.0 dS·m-1 electrical conductivity under a completely randomized design with four replications. Shoot length, shoot and root dry mater, leaf area, stem-cross-sectional area, relative chlorophyll content (SPAD), and leaf N, P, K, Ca, Mg, Mn, Zn, Cu, Fe and Na concentration were evaluated The presence of NaCl in the irrigation water caused inhibition of growth in young plants. Significant reduction in contents of chlorophyll was also detected. Tissue concentration of N and Na increased with increasing levels of NaCl, while K, Zn, Cu and Fe decreased. Ca, Mg, P and Mn, were not affected. On the basis of data obtained, papaya ‘Maradol’ can be considered as a moderately NaCl tolerant cultivar.

  19. Influencia del Color y Estados de Madurez sobre la Textura de Frutas Tropicales (Mango, Papaya y Plátano) / Influence of Color and Maturity Stages on the Texture of Tropical Fruits (Mango, Papaya and Plantain)

    Scientific Electronic Library Online (English)

    Ramiro, Torres; Everaldo J, Montes; Omar A, Pérez; Ricardo D, Andrade.

    Full Text Available En este estudio se determinó la influencia del estado de madurez sobre las propiedades texturales de mango variedades Puerco y Corazón, de papaya variedad Hawaiana y de plátano. Se correlacionaron los parámetros texturales con el color medido en el espacio CIELAB, mediante un Análisis de Perfil de T [...] extura (dureza, gomosidad, adhesividad, elasticidad, cohesividad y masticabilidad), utilizando un texturómetro con plato de compresión de 50 mm y una velocidad de compresión de 1 mm/s. La dureza, gomosidad y masticabilidad pueden correlacionarse con una ecuación polinómica de segundo grado en °HUE, para el mango (Mangifera indica L) variedad Puerco y Corazón; la dureza y gomosidad para la papaya (Carica papaya L.) variedad Hawaiana y con la dureza para plátano (Mussa Cavendish). Abstract in english The influence of the stage of maturity on the textural properties of Mango varieties Pork and Heart, of papaya of Hawaiian variety and plantain were determined in this study. Texture Profile Analysis (hardness, gumminess, adhesiveness, springiness, cohesiveness and chewiness) was performed with a te [...] xturometer with a compression plate of 50 mm and a compression rate of 1 mm /s. Hardness, gumminess and chewiness are well correlated with a second degree polynomial in °HUE for mango (Mangifera indica L) variety Puerco and Corazón; hardness and gumminess for papaya (Carica papaya L.) variety Hawaiana y with hardness for plantain (Mussa Cavendish).

  20. Conocimientos y autoeficacia asociados a la prevención del VIH y SIDA en mujeres chilenas

    Science.gov (United States)

    Villegas Rodríguez, Natalia; Ferrer Lagunas, Lilian Marcela; Cianelli Acosta, Rosina; Miner, Sarah; Lara Campos, Loreto; Peragallo, Nilda

    2014-01-01

    Resumen Objetivo Evaluar la relación existente entre conocimientos y autoeficacia asociados al VIH/SIDA en mujeres chilenas en desventaja social. Metodología Estudio correlacional, que utiliza la medición basal del estudio “Testeando una intervención en VIH y SIDA en mujeres chilenas”, realizada entre 2006 y 2008, que tiene una muestra de 496 mujeres entre 18 y 49 años residentes en dos comunas de Santiago de Chile. Las participantes respondieron un cuestionario estructurado aplicado por entrevistadoras entrenadas. Este cuestionario incluyó preguntas sobre datos sociodemográficos, escala de conocimientos de conductas de riesgo y autoeficacia, entre otros. Resultados Edad promedio de 32.3±9.1 años, 72.2% vive con su pareja y 42.7% poseen educación media completa. La puntuación media de los conocimientos de la infección por el VIH fue de 8.9±2.5, mientras que para las tres escalas empleadas para medir autoeficacia fueron: “Normas de los pares” =9.8±3.6, “Intención de reducir conductas de riesgo” =12.2±3.6 y “Self Efficacy Form”=20.2±4.7. Los conocimientos tuvieron una correlación positiva débil con la “intención de reducir conductas de riesgo” (r=0.19; prelaciones sexuales seguras” (r=0.13; p=0.78). Conclusión Existe una débil correlación positiva entre el nivel de conocimientos sobre el VIH/SIDA y la autoeficacia en mujeres chilenas en desventaja social. PMID:25284914

  1. Protective role of Carica papaya (Linn.) in electron beam radiation induced hematological and cytogenetic damages in Swiss albino mice

    International Nuclear Information System (INIS)

    Carica papaya (Linn.) is known to possess various biomedical applications. It has remarkable antioxidant properties. The main objective of the study was to evaluate the leaf extracts of Carica papaya (Linn.) on hematologic and cytogenetic changes occurring due to irradiation of mice to sub-lethal doses of Electron Beam Radiation (EBR). Analysis of hematological changes occurring due to irradiation of mice to sub-lethal doses of EBR, and the effects of Carica papaya (Linn.) extract on the same. The Assessment of hematopoietic stress by spleen colony forming unit and spleen body weight index. The analysis of cell proliferation and immunomodulation with response to the effects of Carica papaya (Linn.) extract by estimation of IL-6. The estimation of serum total antioxidants, lipid peroxidation and analyzing the activities of enzymes like SOD, ALP, and AST. Male Swiss albino mice were fed orally with papaya aqueous leaf extract for 15 days. They were irradiated with a whole body dose of 6 Gy Electron Beam radiation. The mice were dissected for liver, kidney, bone marrow, spleen and brain. The hematological studies were done using blood cell count in an automated cell counter. The biochemical estimations like urea, creatinine, SGOT, SGPT, Total Protein, Albumin, Bilirubin were done using the serum and homogenates. The total antioxidant capacity, the antioxidant enzymes were estimated. The Interleukin-6 levels were estimated in serum to assess immune modulation. The results show a decrease in the hematological parameters in radiated animals. The papaya treated groups have shown modulation in the hematological parameters. The extract has also reduced the suppression of the bone marrow induced by radiation. The radiation induced liver damage is also reduced in papaya treated groups. The aqueous extract of Carica papaya (Linn.) has shown protective effects in electron beam radiation induced tissue damages in Swiss Albino mice (author)

  2. n vitro study of antiamoebic effect of methanol extract of mature seeds of Carica papaya on trophozoites of Entamoeba histolytica

    Directory of Open Access Journals (Sweden)

    Sujit Kumar Sarker, Nilufar Begum, Dinesh Mondal, Md. Abdullah Siddique, Mohammad A. Rashid

    2010-06-01

    Full Text Available Antiamoebic activity of methanol extract of mature seeds of Carica papaya was tested in vitro on axenic culture of Entamoeba histolytica using metronidazole as a reference amoebicidal agent. The MIC of seed extract was > 62.5 µg/mL as compared to < 0.8 µg/mL for metronidazole. The present study suggests that the mature seeds of C. papaya have antiamoebic effect but less pronounced than metronidazole.

  3. Oral dosing with papaya latex is an effective anthelmintic treatment for sheep infected with Haemonchus contortus

    OpenAIRE

    Donnan Alison A; Garnett Martin C; Bartley David J; Elsheikha Hany M; Bartley Yvonne; Behnke Jerzy M; Buttle David J; Jackson Frank; Lowe Ann; Duce Ian R

    2011-01-01

    Abstract Background The cysteine proteinases in papaya latex have been shown to have potent anthelmintic properties in monogastric hosts such as rodents, pigs and humans, but this has not been demonstrated in ruminants. Methods In two experiments, sheep were infected concurrently with 5,000 infective larvae of Haemonchus contortus and 10,000 infective larvae of Trichostrongylus colubriformis and were then treated with the supernatant from a suspension of papaya latex from day 28 to day 32 pos...

  4. Estudos morfo-anatômicos de sementes de dois genótipos de mamão(Carica papaya L.) / Morphological and anatomical studies of seeds of two genotypes of papaya Carica papaya L.

    Scientific Electronic Library Online (English)

    Sônia Aparecida dos, Santos; Roberto Ferreira da, Silva; Messias Gonzaga, Pereira; Eduardo, Alves; José da Cruz, Machado; Flávio Meira, Borém; Renato Mendes, Guimarães; Elizabeth Rosemeire, Marques.

    Full Text Available A propagação do mamoeiro Carica papaya L. é realizada por meio de mudas oriundas das sementes. Apesar da importância da semente do mamoeiro para a formação dos pomares comerciais, estudos morfológicos e anatômicos neste sentido são raros. O presente trabalho descreve aspectos morfológicos e anatômic [...] os das estruturas que constituem as sementes de dois genótipos do mamoeiro, Sunrise Solo 783 e Formosa Roxo 45. Sunrise Solo 783 e Formosa Roxo 45 pertencem aos dois grandes grupos 'Solo' e 'Formosa' respectivamente. Os referidos genótipos foram oriundos do banco de germoplasma da Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF) em parceria com Empresa Caliman Agrícola S.A. A pesquisa foi conduzida no Laboratório de Microscopia Eletrônica da Universidade Federal de Lavras (UFLA), Lavras, MG.Para a caracterização morfológica procedeu-se a dissecação e observação das estruturas utilizando bisturi, pinças e sexploradora sob microscópio estereoscópio. Para a caracterização anatômica utilizou-se a microscopia eletrônica de varredura. As amostras foram preparadas de acordo com a metodologia de rotina do Laboratório de Microscopia Eletrônica da UFLA e observadas a alto vácuo em diversos aumentos no microscópio Leo Evo 40, à distância de trabalho de 10 mm. As medições foram realizadas usando-se o Software Leo User Interface (versão Leo 3.2). Os estudos morfológicos permitiram identificar as estruturas que compõem a semente do mamoeiro e os estudos anatômicos permitiram observar a existência de diferenças estruturais e dimensionais entre as células dos dois genótipos estudados. Abstract in english The papaya (Carica papaya L.) propagation is made by seedlings produced from true seeds. Although the use of seeds in the implementation of commercial papaya orchards is well documented in literature, studies on the anatomy and morphology of these structures are in small number. In present work morp [...] hological and anatomical aspects of the seeds from two papaya genotypes Sunrise Solo 783 and Formosa Roxo 45,belonging to two grand groups ´Solo` and ´Formosa` are described. These genotypes were obtained of Gemoplasm Bank of the Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF) in cooperation with the company Caliman Agricola S.A. The work was carried out at the Eletronic Microscopy and Ultra-strutural Analisys Laboratory of the Universidade Federal de Lavras (UFLA). To make the morphological characterization, the seeds were dissected carefully by hand and observed in the stereomicroscope. For the anatomic studies, seeds were prepared according to the routine protocol of the LME and observed in scanning electronic microscope Leo Evo 40, at work distance of 10 mm under high vacuum conditions. Measurements were made using the Software Leo User Interface (Version Leo 3.2). The results of the morphological studies provide adequate conditions to observe the different structures of the papaya seeds and from anatomic studies it was possible to verify structural and dimensional differences among the cells of the two genotypes under investigation.

  5. Estudos morfo-anatômicos de sementes de dois genótipos de mamão(Carica papaya L. Morphological and anatomical studies of seeds of two genotypes of papaya Carica papaya L.

    Directory of Open Access Journals (Sweden)

    Sônia Aparecida dos Santos

    2009-01-01

    Full Text Available A propagação do mamoeiro Carica papaya L. é realizada por meio de mudas oriundas das sementes. Apesar da importância da semente do mamoeiro para a formação dos pomares comerciais, estudos morfológicos e anatômicos neste sentido são raros. O presente trabalho descreve aspectos morfológicos e anatômicos das estruturas que constituem as sementes de dois genótipos do mamoeiro, Sunrise Solo 783 e Formosa Roxo 45. Sunrise Solo 783 e Formosa Roxo 45 pertencem aos dois grandes grupos 'Solo' e 'Formosa' respectivamente. Os referidos genótipos foram oriundos do banco de germoplasma da Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF em parceria com Empresa Caliman Agrícola S.A. A pesquisa foi conduzida no Laboratório de Microscopia Eletrônica da Universidade Federal de Lavras (UFLA, Lavras, MG.Para a caracterização morfológica procedeu-se a dissecação e observação das estruturas utilizando bisturi, pinças e sexploradora sob microscópio estereoscópio. Para a caracterização anatômica utilizou-se a microscopia eletrônica de varredura. As amostras foram preparadas de acordo com a metodologia de rotina do Laboratório de Microscopia Eletrônica da UFLA e observadas a alto vácuo em diversos aumentos no microscópio Leo Evo 40, à distância de trabalho de 10 mm. As medições foram realizadas usando-se o Software Leo User Interface (versão Leo 3.2. Os estudos morfológicos permitiram identificar as estruturas que compõem a semente do mamoeiro e os estudos anatômicos permitiram observar a existência de diferenças estruturais e dimensionais entre as células dos dois genótipos estudados.The papaya (Carica papaya L. propagation is made by seedlings produced from true seeds. Although the use of seeds in the implementation of commercial papaya orchards is well documented in literature, studies on the anatomy and morphology of these structures are in small number. In present work morphological and anatomical aspects of the seeds from two papaya genotypes Sunrise Solo 783 and Formosa Roxo 45,belonging to two grand groups ´Solo` and ´Formosa` are described. These genotypes were obtained of Gemoplasm Bank of the Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF in cooperation with the company Caliman Agricola S.A. The work was carried out at the Eletronic Microscopy and Ultra-strutural Analisys Laboratory of the Universidade Federal de Lavras (UFLA. To make the morphological characterization, the seeds were dissected carefully by hand and observed in the stereomicroscope. For the anatomic studies, seeds were prepared according to the routine protocol of the LME and observed in scanning electronic microscope Leo Evo 40, at work distance of 10 mm under high vacuum conditions. Measurements were made using the Software Leo User Interface (Version Leo 3.2. The results of the morphological studies provide adequate conditions to observe the different structures of the papaya seeds and from anatomic studies it was possible to verify structural and dimensional differences among the cells of the two genotypes under investigation.

  6. Morphological study of bone marrow to assess the effects of lead acetate on haemopoiesis and aplasia and the ameliorating role of Carica papaya extract

    OpenAIRE

    THAM, CHING S.; Chakravarthi, Srikumar; Haleagrahara, Nagaraja; DE ALWIS, RANJIT

    2012-01-01

    Lead causes damage to the body by inducing oxidative stress. The sites of damage include the bone marrow, where marrow hypoplasia and osteosclerosis may be observed. Leaves of Carica papaya, which have antioxidant and haemopoietic properties, were tested against the effect of lead acetate in experimental rats. The rats were divided into 8 groups; control, lead acetate only, Carica papaya (50 mg and 200 mg), post-treatment with Carica papaya (50 mg and 200 mg) following lead acetate administra...

  7. Metabolismo de carboidratos durante o amadurecimento do mamão (Carica papaya L. Cv. Solo): influência da radiação gama / Influence of gamma radiation on carbohydrates metabolism of ripening papaya (Carica papaya L. cv. Solo)

    Scientific Electronic Library Online (English)

    M. L. P. A., GOMEZ; F. M., LAJOLO; B. R., CORDENUNSI.

    1999-05-01

    Full Text Available A irradiação de alimentos é um tratamento pós-colheita bastante promissor, capaz de atuar tanto na desinfestação como no aumento da vida útil de frutos. O mamão é um fruto com curta vida pós-colheita, levando de 6 a 12 dias para atingir a senescência. Além disto, a infestação por larvas de mosca dim [...] inui sensivelmente o seu potencial de exportação. O objetivo deste trabalho foi verificar a ação de uma dose de 0,5 kGy de irradiação gama, normalmente utilizada para desinfestação e aumento de vida útil dos frutos, sobre o comportamento dos carboidratos solúveis durante o amadurecimento do mamão (Carica papaya L. cv. Solo), e sobre as enzimas relacionadas ao metabolismo da sacarose: sacarose sintase (SS), sacarose-fosfato sintase (SPS) e invertases ácida e neutra. Os resultados demonstraram que a irradiação não exerce efeito sobre o teor de glicose e frutose, ou sobre a respiração e atividade da SS. Entretanto, a composição dos açúcares solúveis totais e da sacarose, e a atividade da SPS e das invertases foram afetados. Abstract in english Food irradiation is one of the most promising treatments that can be utilized for fruits desinfestation and extension of shelf life. The authors studied the influence of 0,5 kGy of Gamma irradiation on the soluble carbohydrates composition of papaya (Carica papaya L. cv. Solo) fruit, and on sucrose [...] metabolizing enzymes: sucrose synthase (SS), sucrose-phosphate synthase (SPS), acid and neutral invertases activities, during ripening. The results demonstred that ethylene production, total soluble sugars, sucrose content, and sucrose-phosphate synthase and invertases activities were affected by irradiation, but not respiration, glucose and fructose content, and SS activity.

  8. Comparisons of Production Costs and Profit of Three Different Technology Levels of Papaya Production in Tabasco, Mexico

    Directory of Open Access Journals (Sweden)

    E. Guzmán-Ramón

    2008-04-01

    Full Text Available The survey was carried out from September 2006 to January 2007 in three papaya production sites located in main papaya production zones in Tabasco; SE Mexico. There are differences in size of the cultivated area, in the yield of the papaya as well as in production costs and profit, according to the different technology levels in the farming systems: low, medium and high technology cultivation level. The financial evaluations were carried out in three sites with different productive technologies. The comparison of the agronomic and economic traits results for low technology level in: V AN of 2359.00 USD, BCR in 1.9 and an equilibrium point of 3750.00 USD, TIR of 0.25. In order to avoid loses, a quantity of 10714 kg papaya should be sold. In medium technology VAN is 1605.10 USD, BCR is 1.7, TIR 0.20 and the equilibrium point is 12800.00 USD. 36571 kg of papaya should be yearly sold. In high technology level VAN is 11749.40, BCR is 2.73, TIR 0.43 and the equilibrium point is 12187.50 USD, 34821 kg papaya should be sold yearly. The indicators showed that all three levels are profitable and economically viable.

  9. Population dynamics and within-plant distribution of the mite Calacarus flagelliseta (Acari: Eriophyidae) on papaya in Hawaii.

    Science.gov (United States)

    Fournier, Valerie; Rosenheim, Jay A; Brodeur, Jacques; Johnson, Marshall W

    2004-10-01

    An important element in developing a management strategy for a new pest is the study of its seasonal dynamics and within-plant distribution. Here, we studied the mite Calacarus flagelliseta Fletchmann, De Moraes & Barbosa on papaya, Papaya carica L. (Caricaceae), in Hawaii to quantify 1) patterns of seasonal abundance, 2) its distribution across different vertical strata of the papaya canopy, and 3) shifts in its use of the upper versus the lower surfaces of papaya leaves. Nondestructive sampling conducted in two papaya plantings revealed that 1) populations of C. flagelliseta peak during the summer; 2) mites are most abundant in the middle and lower strata of the plant canopy, and least abundant on the youngest leaves found in the upper canopy; and 3) mites are found more predominantly on the upper leaf surfaces when overall population density peaks, suggesting that individuals move from the lower to the upper leaf surfaces when food resources on the lower leaf surface have been exploited by conspecifics. These results have significant implications for the development of sampling plans for C. flagelliseta in papaya. PMID:15568344

  10. Ultrasound-assisted extraction (UAE) and solvent extraction of papaya seed oil: yield, fatty acid composition and triacylglycerol profile.

    Science.gov (United States)

    Samaram, Shadi; Mirhosseini, Hamed; Tan, Chin Ping; Ghazali, Hasanah Mohd

    2013-01-01

    The main objective of the current work was to evaluate the suitability of ultrasound-assisted extraction (UAE) for the recovery of oil from papaya seed as compared to conventional extraction techniques (i.e., Soxhlet extraction (SXE) and solvent extraction (SE)). In the present study, the recovery yield, fatty acid composition and triacylglycerol profile of papaya seed oil obtained from different extraction methods and conditions were compared. Results indicated that both solvent extraction (SE, 12 h/25 °C) and ultrasound-assisted extraction (UAE) methods recovered relatively high yields (79.1% and 76.1% of total oil content, respectively). Analysis of fatty acid composition revealed that the predominant fatty acids in papaya seed oil were oleic (18:1, 70.5%-74.7%), palmitic (16:0, 14.9%-17.9%), stearic (18:0, 4.50%-5.25%), and linoleic acid (18:2, 3.63%-4.6%). Moreover, the most abundant triacylglycerols of papaya seed oil were triolein (OOO), palmitoyl diolein (POO) and stearoyl oleoyl linolein (SOL). In this study, ultrasound-assisted extraction (UAE) significantly (p < 0.05) influenced the triacylglycerol profile of papaya seed oil, but no significant differences were observed in the fatty acid composition of papaya seed oil extracted by different extraction methods (SXE, SE and UAE) and conditions. PMID:24152670

  11. Ultrasound-Assisted Extraction (UAE and Solvent Extraction of Papaya Seed Oil: Yield, Fatty Acid Composition and Triacylglycerol Profile

    Directory of Open Access Journals (Sweden)

    Hasanah Mohd Ghazali

    2013-10-01

    Full Text Available The main objective of the current work was to evaluate the suitability of ultrasound-assisted extraction (UAE for the recovery of oil from papaya seed as compared to conventional extraction techniques (i.e., Soxhlet extraction (SXE and solvent extraction (SE. In the present study, the recovery yield, fatty acid composition and triacylglycerol profile of papaya seed oil obtained from different extraction methods and conditions were compared. Results indicated that both solvent extraction (SE, 12 h/25 °C and ultrasound-assisted extraction (UAE methods recovered relatively high yields (79.1% and 76.1% of total oil content, respectively. Analysis of fatty acid composition revealed that the predominant fatty acids in papaya seed oil were oleic (18:1, 70.5%–74.7%, palmitic (16:0, 14.9%–17.9%, stearic (18:0, 4.50%–5.25%, and linoleic acid (18:2, 3.63%–4.6%. Moreover, the most abundant triacylglycerols of papaya seed oil were triolein (OOO, palmitoyl diolein (POO and stearoyl oleoyl linolein (SOL. In this study, ultrasound-assisted extraction (UAE significantly (p < 0.05 influenced the triacylglycerol profile of papaya seed oil, but no significant differences were observed in the fatty acid composition of papaya seed oil extracted by different extraction methods (SXE, SE and UAE and conditions.

  12. Eficiência fotoquímica em folhas do mamoeiro (Carica papaya L.) cv. Golden durante o estádio reprodutivo e caracterização da ontogenia dos frutos / Photochemical efficiency of papaya leaves (Carica papaya L.) cv. Golden during the reproductive stage and characterization of fruit ontogeny

    Scientific Electronic Library Online (English)

    Diolina Moura, Silva; Ketney Tonetto dos, Santos; Maísa Melo, Duarte.

    2011-12-01

    Full Text Available Considerando que a fisiologia do amadurecimento dos frutos do mamoeiro envolve desde a produção de fotoassimilados, para a sua formação e desenvolvimento, até as enzimas que degradarão a parede celular, promovendo o amolecimento da polpa, o objetivo deste trabalho foi avaliar a cinética da emissão d [...] a fluorescência da clorofila a em folhas do mamoeiro (Carica papaya L.) cv. Golden durante o período reprodutivo e avaliar os teores de sólidos solúveis (SS), acidez titulável (AT) e atividade da pectinametilesterase (PME) durante a ontogenia dos frutos. Os maiores índices de desempenho fotoquímico (PItotal) foram verificados nos estádios de formação e crescimento dos frutos, devido ao melhor desempenho das reações de oxirredução do fotossistema I [?R0/(1-?R0)]. Maior eficiência fotoquímica das plantas neste período possibilita maior demanda pelo carbono fixado para a síntese de compostos para o metabolismo celular. Em geral, os fotoassimilados são direcionados para os drenos fortes mais próximos, entretanto as sementes têm prioridade, portanto os teores de SS, AT e a atividade da PME na polpa dos frutos permaneceram constantes durante o desenvolvimento dos mesmos, sendo verificadas alterações nestas características somente no estádio de colheita. Estes resultados confirmam a participação da PME como enzima que prepara o substrato para que as demais enzimas pectinolíticas atuem, disponibilizando os ácidos orgânicos desmetilados e o início da degradação dos polissacarídeos da parece celular antes mesmo da colheita. Abstract in english Whereas the physiology of fruit ripening of papaya involves from the production of photoassimilates for their training and development to the enzymes that degrade the cell wall promoting pulp softening, the objective of this study was to evaluate the chlorophyll a (Chl a) fluorescence induction kine [...] tics in of papaya (Carica papaya L.) cv. Golden leaves during the reproductive period and to evaluate the total soluble solids (SS), acidity total titratable (ATT), and activity of pectin methyl esterase (PME) during fruit ontogeny. The highest rates of photochemical performance index (PI total) were observed in the stages of fruit formation and growth , due to better performance of the redox reactions of photosystem I [?R0 / (1-?R0)]. Higher photochemical efficiency of plants in this period allows greater demand for fixed carbon for the synthesis of compounds for cellular metabolism. Generally, photoassimilates are directed to the closer strong drains, but the seeds have priority, so the SS, AT and PME activity in flesh fruit, remained constant during their development, and any alterations in these characteristics only in the harvest stage. These results confirm that the PME as an enzyme that prepares the substrate for the other pectic enzymes act, providing the demethylated organic acids and the beginning of the degradation of cellular wall polysaccharides even before harvest.

  13. Inoculação de fungo micorrízico e utilização de substratos comerciais para produção de plântulas de mamoeiro (Carica papaya L.) / Inoculation of mycorrhizal fungi and utilization of commercial substrates to papaya seedlings (Carica papaya L.) production

    Scientific Electronic Library Online (English)

    João P.N., Almeida; Bruno F.T., Lessa; Emanoela P., Paiva; Ítalo G., Arrais; Mauro S., Tosta; Vander, Mendonça.

    2014-09-01

    Full Text Available O cultivo do mamoeiro (Carica papaya) está entre as principais atividades do setor frutícola mundial. Métodos de gestão que efetivem o desenvolvimento da cultura são fundamentais. Assim, este trabalho teve por objetivos estudar o crescimento de plântulas de mamoeiro sob diferentes substratos comerci [...] ais com inoculação (em semente) de fungo micorrízico arbuscular. As sementes de mamão foram inoculadas com o fungo Glomus fasciculatum, em três dosagens: 0,0 g, 1,0 g e 2,0 g para cada 10,0 g de sementes. De seguida, as sementes foram colocadas em tabuleiros de poliestireno com quatro diferentes substratos comerciais. O estudo foi realizado em esquema fatorial com delineamento inteiramente casualizado com cinco repetições. Trinta dias após a sementeira fizeram-se as análises de crescimento e desenvolvimento das plantas. O substrato comercial que continha na sua composição superfosfato simples, nitrato de potássio, turfa, vermiculita e casca de Pinus proporcionou um maior crescimento das plântulas. A inoculação das sementes com a dose de 2,0 g do fungo micorrízico beneficiou o crescimento das plântulas. Abstract in english Papaya (Carica papaya) cultivation is among the principal activities of the world orchard sector. Therefore, it becomes important to find ways of management that improve the development of the culture. This work aimed to study the growth and the development of papaya seedlings in different commercia [...] l substrates with inoculation (in seeds) of the arbuscular mycorrhizal fungi Glomus fasciculatum, in three dosages: 0.0 g, 1.0 g, and 2.0 g for each 10.0 g of seeds. The seeds were placed in polystyrene trays with four different commercial substrates. The experiment was performed in factorial scheme in completely randomized design, with five replications. Thirty days after seedling, plant growth and development were analyzed. The commercial substrate containing superphosphate, potassium nitrate, turf, vermiculite and Pinus bark provided the highest growth of seedlings. The inoculation of seeds with 2.0 g of mycorrhizal fungi benefits the growth of seedlings.

  14. Anatomia foliar de plantas transgênicas e não transgênicas de Carica papaya L. (Caricaceae Leaf anatomy of genetically modified and wild-type Carica papaya L. (Caricaceae

    Directory of Open Access Journals (Sweden)

    Marcos Vinicius Leal-Costa

    2010-06-01

    Full Text Available O mamoeiro, Carica papaya L. (Caricaceae é uma espécie americana, cujos frutos são largamente consumidos em todo mundo. Devido às perdas de produção provocadas por viroses e a dificuldade em controlá-las por métodos convencionais, a espécie tem sido alvo de pesquisas de melhoramento genético envolvendo transgenia para resistência a vírus. O presente trabalho descreve a anatomia foliar de plantas de mamoeiro convencional e transgênico resistente ao vírus da mancha anelar-Papaya ringspot virus (PRSV com inserção da capa protéica viral. As duas cultivares apresentam pecíolo com endoderme e fibras pericíclicas. As folhas são hipoestomáticas e dorsiventrais, com laticíferos acompanhando os feixes vasculares e grande concentração de idioblastos com drusas de oxalato de cálcio. A epiderme é glabra, possuindo estômatos anomocíticos e anisocíticos, com células de paredes anticlinais retas na face adaxial e levemente sinuosas na face abaxial. O presente trabalho concluiu que o processo de transformação genética não alterou as características anatômicas das folhas de C. papaya, servindo de subsídio para avaliação da conformidade anatômica da cultivar transgênica.Papaya, Carica papaya L. (Caricaceae, is an American species, consumed worldwide. A major limitation to papaya production is attack by viruses, like the papaya ringspot virus (PRSV. Papaya has been genetically modified to increase its resistance to PRSV. The aim of this research was to compare the leaf anatomy of wild-type and genetically modified (GM C. papaya plants to evaluate the influence of genetic modification on leaf anatomy. Wild-type and GM plants showed petiole with endodermis and pericycle fibers. The leaves are hypostomatic and dorsiventral, with laticifers along vascular system and abundant druses of calcium oxalate. The epidermis was glabrous and presented anomocytic and anisocytic stomata, straight anticlinal walls on the adaxial face and sinuous on the abaxial face. Anatomical differences between wild-type and GM C. papaya leaves were not observed. These data contribute to risk assessments regarding the anatomical conformity of GM plants.

  15. Radiation induced mutation to develop dwarf and precocious lines of papaya

    International Nuclear Information System (INIS)

    Papaya (Carica papaya L.) is one of the most important fruit cultivated throughout the tropical and subtropical regions of the country. Almost all the varieties of papaya developed either through selection or hybridization. As regards mutation breeding, very little work has been done in India. Only one variety PusaNanha has been developed through mutation breeding by treating the seed of papaya strain Pusa 1-15 with 15Kr gamma rays. Mutation breeding is the most appropriate for improving one or two easily identifiable traits in an otherwise well accepted breeding lines or commercial variety. Dwarfness and earliness in flowering are important traits in high density planting as breeding objective for improving papaya varieties for high yield with medium size fruits and good fruit quality. With these objectives, seeds of the papaya P 7-2 were treated with gamma rays 0.1, 0.15, 0.2, 0.25 and 0.3 kGy. The control recorded maximum germination (68%). Among the other treatments maximum germination (64.25%) was recorded at 0.1 kGy. The germination percentage was observed to be least (45%) at 0.3 kGy. Minimum length of seedlings (8.5 cm) and diameter (3.92 mm) was recorded in treatment 0.1 kGy while maximum length (19.2 cm) and diameter (6.26 mm) was recorded in treatment 0.3 kGy after 30 days of sowing. Minimum height of the plant (79.24 cm) was recorded in treatment 0.1 kGy while maximum (112.20) in control. Minimum plant girth (33.40 cm) was measured in 0.3 kGy while maximum (44.34 cm) in 0.15 kGy treatment. Minimum height at first flower initiation (55.28 cm), days to flower initiation (78.28) and length of petiole (60.45 cm) was noted in treatment 0.1 kGy while maximum height at first flower initiation (78.2 cm), days to flower initiation (87.46) and length of petiole (68.24 cm) was found in control. Among treatments, maximum number (18) of fruit was counted in 0.3 kGy treatment while maximum weight of fruit (750 g) was recorded in control. Maximum TSS (10.6 °Brix) in fruit was found in treatment 0.1 kGy. Dwarf and precocious lines were selected and selfed for raising M2 population. (author)

  16. Sex and aggregation pheromone transport after methyl eugenol consumption in male Bactrocera papayae

    International Nuclear Information System (INIS)

    Amongst at least 52 sibling species complexes in the Oriental fruit fly, Bactrocera dorsalis Hendel (Diptera: Tephritidae), B. papayae (formerly Mal B) Drew and Hancock (Drew and Hancock 1994) is beginning to emerge as an economically important insect pest which poses a severe threat to the fruit cultivation in both subtropical and tropical countries. In Malaysia, B. papayae is one of the most damaging pests which infests many commercially grown fruits (Tan and Lee 1982). Like the Oriental fruit fly and its sibling species complex, B. carambolae Drew and Hancock, B. papayae is also strongly attracted to, and compulsively feeds on, methyl eugenol (ME) (Tan 1993). Chemical analyses revealed that in B. papayae males, ME is converted to phenylpropanoids which are then selectively accumulated in the rectal gland. Of the three major volatile substances, 2-allyl-4,5-dimethoyphenol (allyl-DMP) was detected in higher quantities relative to the trans-coniferyl alcohol (4-(3-hydroxy-E-propenyl)-2-methoxyphenol) (CF) and cis-3,4-dimethoxycinnamyl alcohol (cis-DMC) (Nishida et al. 1988a, 1988b). Behavioural studies have also shown that allyl-DMP and CF function as male sex and aggregation pheromone in B. papayae (Tan and Nishida 1996, Hee and Tan 1998). Allyl-DMP was found to be the most attractive compound and cis-DMC the least attractive to the males (Tan 1996). Consumption of ME enhances the mating competitiveness of males. This is demonstrated by the strong attraction of females to conspecific ME-fed males in wind tunnel experiments (Hee and Tan 1998). In male-male mating competition for virgin females, males that fed on ME performed significantly better (Shelly and Dewire 1994, Tan and Nishida 1996). Thus it appears that ME-fed males produced signals that were more attractive. However, the characterisation and understanding of the functions of these phenylpropanoids have not been accompanied by studies of their physiological mode of transport in male flies. The current paper describes the sex and aggregation pheromone transport after methyl eugenol consumption in male B. papayae. Here, we investigate the presence of the pheromones and their precursor ME in the male digestive and circulatory system

  17. A microsatellite library for Carica papaya L. cv. Sunrise solo / Marcadores microssatélites para Carica papaya L. cv. Sunrise solo

    Scientific Electronic Library Online (English)

    Silvia Correa, Santos; Carlos, Ruggiero; Cristina Lacerda Soares Petrarolha, Silva; Eliana Gertrudes Macedo, Lemos.

    2003-08-01

    Full Text Available Em áreas experimentais das Fazendas de Ensino e Pesquisa da UNESP/Campus de Ilha Solteira e Jaboticabal foram selecionadas e marcadas 20 plantas hermafroditas e 20 femininas dos cultivares Sunrise Solo, Improved Sunrise Solo cv.72/12 e Baixinho de Santa Amália. As sementes provenientes dos frutos se [...] lecionados foram plantadas para analisar-se a eficiência da autofecundação e a freqüência dos sexos nas progênies. Posteriormente, amostras de tecido foliar jovem das plantas matrizes foram coletadas para a extração de DNA. Foram construídas cinco bibliotecas enriquecidas de seqüências microsatélites, utilizando-se as sondas (TCA)10, (TC)13, (GATA)4, (CAC)10 e (TGAG)8. Foi possível o desenvolvimento de primers somente com a biblioteca que utilizou a sonda (TCA)10 . Esta permitiu o desenho de 32 pares de primers. Destes, 31 apresentaram padrão de banda única em agarose Metaphor e em acrilamida. Para o primer S36 foram observadas 2 bandas, mas sem polimorfismo para diferenciação da forma sexual na cultura do mamoeiro. No entanto, estes primers poderão ser testados na investigação de outras características em populações segregantes desta espécie e de espécies afins, análises de germoplasma, identificação de cultivares, evolução parental e marcas em melhoramento assistido. Abstract in english In experimental areas of the Education and Researches Ilha Solteira and Jaboticabal UNESP/Campus farms were selected and tagged 20 hermaphrodite plants and 20 feminine of cultivar Sunrise Solo, Improved Sunrise Solo cv.72/12 and Baixinho of Santa Amália.The seeds origined of the selected fruits were [...] cropped to be analysed the self-pollination efficiency and frequency of the sex in the progenies. After that, samples of the young leaf of the matrix plants were colected for the extration of the DNA. It was built five library enriched of microsatellite sequencies, using probes (TCA)10, (TC)13, (GATA)4, (CAC)10 e (TGAG)8.It was possible the development of the primers only in the library that has utilized the probe (TCA)10. This probe allowed the design of 32 primer pairs. From these, 31 presented pattern of unique band in agarose Metaphor and in acrilamide. For primer S36 were observed 2 bands, but with no polymorphism to differentiation in the sexual form at papaya tree culture. However, these primers can be tested, in the futures, in the investigation of the others features in segregated populations of this specie and the related species, germoplasm analysis, cultivars identification, parent evolution and molecular markers for the assisted plant breeding programs.

  18. Control de dos especies de Colletotrichum causantes de antracnosis en frutos de papaya Maradol / Control of two species of Colletotrichum causing anthracnose in Maradol papaya fruits

    Scientific Electronic Library Online (English)

    Felipe, Santamaría Basulto; Raúl, Díaz Plaza; Omar, Gutiérrez Alonso; Jorge, Santamaría Fernández; Alfonso, Larqué Saavedra.

    2011-10-01

    Full Text Available La antracnosis es una limitante de la vida útil de los frutos de papaya, es causada principalmente por Colletotrichum gloeosporioides, aunque es posible encontrar otras especies de Colletotrichum causando enfermedades en un mismo cultivo. Para el control de la antracnosis en Yucatán se recomiendan v [...] arios fungicidas, pero no todos están permitidos por la agencia de protección al ambiente (EPA) de los Estados Unidos de América, principal país al cual se exporta la papaya yucateca. El presente estudio tuvo como objetivos identificar el agente causal de la antracnosis de papaya en Yucatán, evaluar el efecto de fungicidas que cuentan con registro de EPA sobre las especies que causan antracnosis y explorar el efecto de inductores a resistencia en el control de esta enfermedad. Durante marzo y mayo de 2006 se obtuvieron nueve aislamientos de Colletotrichum en frutos de tres regiones productoras de Yucatán, los cuales se identificaron mediante claves taxonómicas. La evaluación de los productos se hizo mediante bioensayos de sensibilidad in vitro y la efectividad en frutos inoculados, posteriormente en agosto de 2007, noviembre de 2007 y febrero de 2008, se evaluó el efecto de la aplicación poscosecha de los fungicidas e inductores de resistencia sobre la antracnosis en frutos infectados naturalmente desde campo. En las tres localidades se encontraron dos especies que causan antracnosis identificadas como C. gloeosporioides y C. dematium. En condiciones in vitro el desarrollo de C. gloeosporioides fue inhibido por prochloraz, ferbam, azoxystrobin, tryfloxystrobin y clorotalonil; el desarrollo de C. dematium fue inhibido sólo por prochloraz, ferbam y clorotalonil. En frutos inoculados prochloraz tuvo 100% de efectividad para las dos especies, mientras que azoxystrobin tuvo 87.5% de efectividad para C. gloeosporioides y 3.3% para C. dematium. Abstract in english Anthracnose is a limitation of papaya fruits' life, mainly caused by Colletotrichum gloeosporioides, although it is possible to find other Colletotrichum species causing diseases in the same crop. In order to control anthracnose in Yucatán, several fungicides are recommended, but not all of them are [...] allowed by the Environmental Protection Agency (EPA) of the United States, the main country to which Yucatan's papaya is exported. This study aimed to identify the causal agent of anthracnose of papaya in Yucatan, to evaluate the effect of fungicides that have EPA registration on species that cause anthracnose and to explore the effect of resistance inducers in controlling this disease. During March and May 2006, nine isolates of Colletotrichum were obtained from fruits of three producing regions of Yucatán, which were identifed using taxonomic keys. The product evaluation was done by sensitivity in vitro bioassays and the effectiveness in inoculated fruits, ensuing in August 2007, November 2007 and February 2008, the effect of postharvest application of fungicides and resistance inducers on anthracnose on naturally infected fruits from the field were evaluated. In the three sites, two species that cause anthracnose were found and identified as C. gloeosporioides and C. dematium. Under in vitro conditions, C. gloeosporioides development was inhibited by prochloraz, ferbam, azoxystrobin, tryfloxystrobin and chlorothalonil; C. dematium development was inhibited only by prochloraz, ferbam and chlorothalonil. In inoculated fruits, prochloraz was 100% effective for both species, while azoxystrobin was 87.5% effective in C. gloeosporioides and 3.3% in C. dematium.

  19. Physico-Chemical Properties of Seed Oil from Papaya (Carica papaya) and the Kinetics of Degradation of the Oil During Heating

    OpenAIRE

    G. Bouanga-Kalou; L. Matos, J.M. Nzikou, F.B. Ganongo-Po, K.E. Malela, M. Tchicaillat-Landou, R.M. Bitsangou, Th. Silou and S. Desobry

    2011-01-01

    The aim of the study was to investigate the physico-chemical properies and the kinetics of degradation of papaya seed oil during heating. The seed is a good source of oil (32%). The physical properties of the oil extracts showed the state to be liquid at room temperature and indicated that the oil had refractive index, 1.4680; the peroxide value, 0.05 (meq O2/kg oil); free fatty acid, 1.2%; iodine value, 72.78 and saponification value, 198.5. Gas liquid chromatography technique has been devel...

  20. Determinação de isotiocianato de benzila em Carica papaya utilizando cromatografia gasosa com detectores seletivos Determination of benzyl isothiocyanate in Carica papaya using gas chromatography with selectives detectors

    OpenAIRE

    Izabela Miranda de Castro; Marianna Ramos dos Anjos; Elba dos Santos de Oliveira

    2008-01-01

    In the present work, a method was developed and validated for the quantification of benzyl isothiocyanate (BITC) in the fruits of Carica papaya. The quantification of this compound was carried out by gas chromatography (GC) with selective detectors - nitrogen phosphorus detector (NPD) and flame photometric detector (FPD). The performance of these detectors showed a higher sensitivity of the NPD with a broader linear range of detection. The LOD/LOQ were 0.038/0.100 µg/mL for NPD and 5.78/19.29...

  1. Studies on physiologically active root distribution and appropriate method of fertilizer application using isotopic techniques in two varieties of papaya (Carica papaya) during pre-bearing stage

    International Nuclear Information System (INIS)

    In Coorg Honey Dew and Surya varieties of papaya aged 10 weeks, the abundance of active roots at 10 cm depth decreased during 20 to 100 days after injection of carrier-free 32P from 77% to 62% in the former and from 89% to 47% in the latter. Bulk of the active roots (85-65%) resided between 10 to 50 cm radial distance. While the phosphorus derived from fertilizer (Pdff) 32P-labelled superphosphate varied from 0.72 to 10.27%, placement between 10 and 40 cm radial distance was appropriate. (author)

  2. Uso de esterco associado à adubação mineral na produção de mudas de mamoeiro (Carica papaya L. The use of manure associated with mineral fertilization in papaya (Carica papaya L. seedling production

    Directory of Open Access Journals (Sweden)

    Regina Célia Faria Simão Canesin

    2006-12-01

    Full Text Available Na composição do substrato para produção de mudas de mamoeiro, recomenda-se o uso de adubação orgânica, que traz como vantagens a melhoria das características físicas, químicas e biológicas do solo, criando um ambiente favorável ao desenvolvimento inicial das mudas, bem como boa resposta do mamoeiro. Os materiais orgânicos desempenham ainda um importante papel na nutrição das plantas como fornecedores de nutrientes. Contudo, observa-se que existe uma grande variação nas recomendações e quase sempre a adubação orgânica está associada à adubação mineral. Neste contexto, o objetivo deste trabalho foi o de verificar os efeitos do uso de esterco de curral associado ou não à adubação mineral no substrato sobre a formação de mudas de mamoeiro. O Experimento foi conduzido na FEP/UNESP/Câmpus de Ilha Solteira-SP. O delineamento utilizado foi em blocos, em esquema de parcelas subdivididas, sendo parcelas as cultivares e subparcelas os substratos. Cada parcela com 5 repetições e 10 plantas úteis por subparcela. Para a comparação das médias, utilizou-se o teste de Tukey. Com base nos resultados obtidos, pode-se concluir que: a Na formação das mudas de mamoeiro, o esterco de curral pode ser utilizado sem a necessidade de adubação mineral com o superfosfato simples e o cloreto de potássio; b O esterco de curral foi capaz de fornecer às mudas de mamoeiro os nutrientes N, P, K, Ca, Mg e Cu, necessários para seu desenvolvimento até o transplantio para o campo.The use of organic fertilization is recommended to be added to the substrate for papaya seedling production, as it enhances the physical, chemical and biological characteristics of the soil, creating a favorable environment for the initial development of the plants, which results in better plant growth. The organic matter has also the important role of providing nutrients to the plants. However, there is great variation in the recommendations, and in most cases organic fertilization is associated with mineral supplementation. The objective of this research was to verify the effects of the use of corral manure, associated or not with mineral fertilization, added to the papaya seedling substrate. The experiment was deployed at the Teaching and Research Farm of the College of Engineering of Ilha Solteira, UNESP, in Ilha Solteira, state of São Paulo, Brazil. The design was set in blocks, in split plots, where papaya cultivars were the whole unit and substrates the subunit treatments. Each unit with 5 replications and the subunit composed of 10 plants each. Means were separated by Tukey test. Results indicated that: a papaya seedling production the corral manure can be used without supplementation of simple superphosphate and potassium chloride, b corral manure alone supplied nutrients N, P, K, Ca, Mg and Cu to the papaya seedlings until transplanted to the field.

  3. Fitotoxicidade de fungicidas, acaricidas e inseticidas sobre o mamoeiro (Carica papaya L.) cultivar sunrise solo improved line 72/12 / Toxic effects of pesticides products on papaya plant (Carica papaya L.) cv. sunrise solo improved line 72/12

    Scientific Electronic Library Online (English)

    Alcílio, Vieira; Carlos, Ruggiero; Sérgio Lucio David, Marin.

    2003-04-01

    Full Text Available Estudaram-se os efeitos fitotóxicos de fungicidas, acaricidas e inseticidas e algumas associações entre eles, em plantas de mamoeiros (Carica papaya L.) cv. Sunrise Solo Improved Line 72/12, em condições de verão, no município de São Mateus - ES, localizado na região produtora do Estado. O experimen [...] to foi arranjado em delineamento de blocos casualizados, com 4 repetições e 03 plantas úteis por parcela experimental. Foram utilizados os seguintes produtos, com as respectivas doses, para cada 100 l de água: abamectin (Vertimec 18 CE - 50 ml); dicofol + tetradiphon (Carbax - 200 ml), fenbutatin oxide (Torque 500 SC - 60 ml); mancozeb (Dithane PM - 200g); oxicloreto de cobre (Reconil - 400g) e thiabendazole (Tecto 450 - 100ml). Analisou-se a fitotoxicidade dos produtos em relação à altura da planta, nº de folhas, número de flores e frutos ; diâmetro do caule e queimaduras ou injúrias foliares. As medições e contagens foram feitas um dia antes das pulverizações, 15 e 30 dias após. Constatou-se que o Vertimec 18 CE, associado ao Reconil ou ao Tecto 450, ocasionou leves injúrias foliares, detectadas aos 15 dias após as pulverizações, que se tornaram praticamente imperceptíveis, aos 30 dias após as pulverizações; e que Dithane PM, Reconil, Tecto 450, Carbax, Torque 500 SC, Dithane PM + Carbax, Dithane PM + Torque 500 SC, Dithane PM + Vertimec 18 CE, Reconil + Carbax, Reconil + Torque 500 SC, Tecto 450 + Carbax e, Tecto 450 + + Torque 500 SC não interferiram nos parâmetros de desenvolvimento e de produção estudados, bem como não causaram injúrias ou queimaduras nas folhas dos mamoeiros. Abstract in english In this work was studied the phytotoxic effect of inseticides, acaricides and fungicides alone and in combination, on papaya plants (Carica papaya L.); cv. Sunrise Solo Improved Line 72/12, under field conditions. The experiment were conducted in a farm, located at São Mateus county, the most import [...] ant papaya region in the State of Espírito Santo.The experiment was arranged in a randomized block design with four replications, with three plants by plot. The products with their respective dose in a volume of 100 l H2O were: abamectin (Vertimec 18 CE - 50 ml); dicofol + tetradifon (Carbax - 200 ml); fenbutatin oxide (Torque 500 SC - 60 ml); mancozeb (Dithane PM - 200g).; cuprix oxychloride (Reconil 400g) and thiabendazole (Tecto 450 - 100ml). Plants were evaluated for plant high, number of leaves, flowers and fruits, and extension of leaf injury, one day before, 15 and 30 days after spray. Vertimec 18 CE in combination with Reconil or Tecto 450, caused light leaf injuries, observed only15 days after spraying. These plants recovered from leaf injury and 30 days later, no leaf lesion was observed. Dithane PM, Reconil, Tecto 450, Carbax, Torque 500 SC, Dithane PM + Carbax , Dithane PM + Torque 500 SC, Dithane PM + Vertimec 18 CE, Reconil + Carbax, Reconil + Torque 500 SC, Tecto 450 + Carbax and Tecto 450 + Torque 500 SC, did not affected plant growth and yield studied. No leaf burns injury were caused in papaya plants.

  4. Effects of irradiation on the organoleptic quality and ripening process of papaya

    International Nuclear Information System (INIS)

    Papaya variety Eksotika harvested at stage 2 of maturity (green skin with a tinge of yellow) were cleaned, air dried and exposed to irradiation at lower doses i.e. 250, 500, 750, 1000 Gray and then were kept at 20 deg. C for up to 9 days or until ripe. Sensory evaluation was carried out the storage period in order to determine effects of radiation on organoleptic quality and ripening process. About 15-24 experienced panelists were involved in the sensory evaluation. It was found that effect on the organoleptic quality of fruit irradiated with dose at 1000 Gray was unacceptable. Nevertheless, the quality was very much less affected by irradiation at 750 Gray or lower. In general the irradiated papaya ripened earlier than the control where the skin colour change by turning from green to 100% yellow but gives harder texture. Freckles on skin surface during ripening were reduced by irradiation

  5. Empleo de un Recubrimiento Formulado con Propóleos para el Manejo Poscosecha de Frutos de Papaya (Carica papaya L. cv. Hawaiana Use of a Coating Formulated with Propolis for Postharvest Handling of Papaya (Carica papaya L. cv. Hawaiian Fruits

    Directory of Open Access Journals (Sweden)

    Elizabeth Barrera Bello

    2012-06-01

    Full Text Available Resumen. El fruto de papaya es reconocido por las propiedades nutricionales y sensoriales; no obstante, su tiempo de vida útil en poscosecha es muy corto. En los últimos años, para incrementar la vida de almacenamiento del fruto se ha implementado el uso de recubrimientos como vehículos de agentes antimicrobianos y antioxidantes, entre otros. En este trabajo se comparó el efecto de dos recubrimientos; cera comercial (control y cera comercial conteniendo un extracto etanólico de propóleos (5% p/v, sobre la vida en poscosecha de frutos de papaya (Carica papaya L. cv. Hawaiana almacenados a temperatura ambiente (28 ± 2 ºC y humedad relativa entre 65 y 70%. El efecto de los recubrimientos se determinó mediante el índice de deterioro de los frutos y el recuento microbiano (mesófilos aerobios, mohos y levaduras. Adicionalmente, se evaluaron durante 12 días las propiedades fisicoquímicas de los frutos (cambio de color, textura, pH, acidez total titulable, pérdida de peso y sólidos solubles. Los resultados mostraron que las papayas tratadas con el recubrimiento formulado con el extracto de propóleos, presentó un menor deterioro en cuanto a su apariencia y mayor inhibición del crecimiento de microorganismos durante los primeros 6 días de evaluación en comparación con los frutos control; además, no se observaron diferencias, producto de los recubrimientos, en relación a las características fisicoquímicas de los frutos.Abstract. Papaya fruit is known for the nutritional and sensory properties; however, its postharvest shelf life is very short. In recent years, to extend the storage life of this fruit, the use of coatings as a carrier of antimicrobial agents and antioxidants, has been implemented. In this work, the effect of two coatings, commercial wax (control and commercial wax containing an ethanolic extract of propolis (5% w/v on the postharvest of papaya (Carica papaya L. cv. Hawaiian was compared. The fruits were stored at room temperature (28 ± 2 ºC and a relative humidity between 65 and 70%. The effect of coatings was determined by the deterioration index of the fruit, and the microbial counts (aerobic mesophiles, molds and yeasts. Additionally, during 12 days were evaluated physicochemical properties of the fruits (color, texture, pH, titratable acidity, weight loss and soluble solids. The results showed that the papaya fruits treated with the coating containing the propolis extract presented less deterioration in their appearance and greater inhibition growth of microorganisms during the first 6 days compared with control fruits; moreover, no differences among the coatings in relation to the physicochemical characteristics of the fruit were observed.

  6. Evaluación de tres cultivares de papaya del grupo Solo basada en caracteres de crecimiento y productividad

    Scientific Electronic Library Online (English)

    Maruchi, Alonso; E, Farrés; Y, Tornet; J, Castro; R, Ramos; María C, Rodríguez.

    2008-06-01

    Full Text Available La papaya (Carica papaya L.) es uno de los frutales más importantes y ampliamente distribuidos en los países tropicales y subtropicales. Debido a la explotación monovarietal basada en el cultivar ‘Maradol’ en Cuba, este cultivo está sujeto a serios riesgos. Para ello, la introducción, caracterizació [...] n y el mantenimiento de nuevos cultivares en el germoplasma de esta especie constituye un factor primordial con fines de identificación y obtención de nuevos genotipos. En el presente trabajo se muestra el comportamiento de tres cultivares de papaya introducidos en el banco de germoplasma de este frutal perteneciente al Instituto de Investigaciones en Fruticultura Tropical. Los resultados indicaron que los cultivares ‘BH-65’ y ‘Baixinho de Santa Amalia’ mostraron diferencias significativas en el carácter altura de la planta con respecto al cultivar ‘Sunset’. El número de hojas totales emitidas por año estuvo por encima de 100, lo que indica una buena adaptabilidad de los genotipos a las condiciones de nuestro ensayo. Los cultivares ‘BH-65’ y ‘Baixinho de Santa Amalia’ emitieron la primera flor a menor altura, carácter que se considera útil con fines productivos. De manera general, se apreció un crecimiento y desarrollo vegetativo favorable, así como una excelente productividad en los tres cultivares de papaya del grupo Solo, lo que representa un gran avance para el desarrollo de futuros programas de mejoramiento y su utilización con fines comerciales. La simple introducción de genotipos del grupo Solo al país puede promover una significativa expansión del cultivo y abrir futuras vías de comercialización de la fruta, debido a su gran aceptación en el mercado de exportación Abstract in english Papaya (Carica papaya L.) is one of the most important and widely spread fruit trees in tropical and subtropical countries. Due to the monovarietal explotation of ‘Maradol’ cultivar in Cuba, this crop is submitted to serious risks. Thus, the introduction, characterization and maintenance of new cult [...] ivars in the germplasm of this species constitute a key factor to identify and obtain new genotypes. This work presents the behaviour of three papaya cultivars introduced in the fruit germplasm bank from the Research Institute of Tropical Fruit Crops. Results indicate that cultivars ‘BH-65’ and ‘Baixinho de Santa Amalia’ showed significant plant height differences compared to ‘Sunset’. More than 100 leaves were emitted per year, which indicates a good genotypic adaptability to the contidions of this trial. Cultivars ‘BH-65’ and ‘Baixinho de Santa Amalia’ emitted the first flower at a lower plant height, a useful feature for productive purposes. In general, all papaya cultivars from Solo group had a favourable growth and vegetative development as well as an excellent productivity. It is a great advance to conduct further breeding programs to be used for commercial purposes. The mere introduction of new cultivars from ‘Solo group’ to the country could promote a crop production increment and therefore open future fruit market ways for exportation due to its trade success

  7. Quality of Guava and Papaya Fruit Pulp as Influenced by Blending Ratio and Storage Period

    Directory of Open Access Journals (Sweden)

    Prabhat K. Nema

    2011-01-01

    Full Text Available Guava and papaya are the most widely grown commercial fruits of central India. Both the fruits are nutritive and may be used for processing. The analysis of organoleptic characters (i.e., colour, flavour, texture, taste and overall acceptability and qualitative characters (i.e., TSS, pH, acidity, ascorbic acid content of guava and papaya fruits was conducted on fresh fruit, prepared pulp and mixed pulp. During the storage of fruit pulp at low temperature (6±1°C, the decrease in overall acceptability of both the pulp was observed with increase in storage period. However, blending of both the pulp in different ratios influenced the organoleptic characters as well as the qualitative characters of the blended pulp.

  8. Enzymatic wound debridement; role of papaya in the management of post cesarean gaped wounds

    International Nuclear Information System (INIS)

    Background: Enzymatic wound debridement is an emerging concept in facilitating the wound healing process. Papaya has de-sloughing, antibacterial and wound healing properties. It has been used in African countries since centuries for different medicinal pur-poses. Apart from anecdotal reports and few studies on chronic ulcers and burns, no planned studies are available to support its action in postoperative wound infection.Objectives: To compare efficacy and safety of papaya dressing with conventional wound dressing with povidone iodine in post cesarean section gaped wounds. Setting: Gynecology Unit 3, Sir Ganga Ram Hospital Lahore - Pakistan, over a period of six months(June 2012 to Nov 2012). Study Design: Randomized, quasi experimental stu-dy. Materials and Methods: The study sample included 60 patients with post cesarean section gaped wounds. The sample was divided into two groups; thirty patients as Group A or the study group received Papaya dressing and rest of thirty patients as Group B or the control group received Povidone iodine dressing. Wounds were thoroughly washed with saline and then mashed unripe papaya was spread over the whole area of wound in the study group and povidone iodine in the control group. Wounds were covered with sterile bandage for at least 48 hours in study group and 24 hours in the control group. The process was repeated till a clean base of wound with healthy granulation tis-sue was achieved suitable for secondary suture. The efficacy parameters studied were the duration of time needed to develop healthy granulation tissue and total duration of hospital stay which were compared bet-ween the two groups. Safety factors studied were the adverse effects of medications used in the study. Results: Out of 1200 cesarean sections done during study period, sixty (5%) were gaped in the post-operative period. Out of 60, 55 (90%) were emergency and only 5 (10%) were elective cesarean sections. All the sixty patients with postoperative gaped wounds were included in the study, of which 30 patients received Papaya and 30 received Povidone iodine dressing. Time required to induce healthy granulation tissue was significantly shorter in the papaya group as compared to povidone iodine group (3.4 +- 0.4 days versus 7.2 +- 0.2 days).Similarly the duration of hospital stay was also short in the study as compared to control group i.e. 11.6 +- 1.2 days and 16.8 +- 1.4 days respectively. The side effects of medications used in study (papaya and povidone iodine) like local irritation and hyper-sensitivity reactions were minor and not significally different in both groups. Conclusion: Papaya dressing is effective, safe, widely available and cost effective for wound healing in post-operative infected gaped wounds. (author)

  9. Structural characterization of the papaya cysteine proteinases at low pH.

    Science.gov (United States)

    Huet, Joëlle; Looze, Yvan; Bartik, Kristin; Raussens, Vincent; Wintjens, René; Boussard, Paule

    2006-03-10

    Current control of gastrointestinal nematodes relies primarily on the use of synthetic drugs and encounters serious problems of resistance. Oral administration of plant cysteine proteinases, known to be capable of damaging nematode cuticles, has recently been recommended to overcome these problems. This prompted us to examine if plant cysteine proteinases like the four papaya proteinases papain, caricain, chymopapain, and glycine endopeptidase that have been investigated here can survive acidic pH conditions and pepsin degradation. The four papaya proteinases have been found to undergo, at low pH, a conformational transition that instantaneously converts their native forms into molten globules that are quite unstable and rapidly degraded by pepsin. As shown by activity measurements, the denatured state of these proteinases which finally results from acid treatment is completely irreversible. It is concluded that cysteine proteinases from plant origin may require to be protected against both acid denaturation and proteolysis to be effective in the gut after oral administration. PMID:16434027

  10. Effects of mechanical wounding on Carica papaya cysteine endopeptidases accumulation and activity.

    Science.gov (United States)

    Azarkan, Mohamed; Dibiani, Rachid; Baulard, Céline; Baeyens-Volant, Danielle

    2006-05-30

    The mechanical wounding impact on the Carica papaya latex protein pattern was investigated by analyzing three latexes. A first one commercially available, a second harvested from unripe but fully grown fruits, both obtained from regularly tapped fruits. A third one was collected from similar fruits but wounded for the first time. The results demonstrated both quantitative and qualitative changes in the protein content and in the enzymatic activity. Repeated wounding results in either, accumulation or activation (or both of them) of papain, chymopapain and caricain. Furthermore, new cysteine protease activity was found to transiently accumulate in the latex collected from newly wounded fruits. The possible implication of this enzymatic material in the papaya cysteine endopeptidases pro-forms activation is discussed. PMID:16580724

  11. Prospects and problems of irradiating papaya. A case study in Hawaii

    International Nuclear Information System (INIS)

    The United States Environmental Protection Agency's (EPA's) ban of ethylene dibromide (EDB) as a fruit fumigant has forced Hawaii's papaya industry to search for alternative treatments. Among various alternatives considered, irradiation process is the most efficacious because fruits can be sorted, packaged, chilled and conveyed to an irradiator for low-dose treatments (0.26 kGy for disinfestation) before shipment to export markets. The papaya industry in Hawaii, however, has not assigned a high priority to the irradiation process. Instead, the industry opted for the double-dip hot water treatment which was rushed to become an USDA-approved procedure shortly before 1 September 1984. Three major concerns expressed by the papaya industry about the irradiation process as a replacement for chemical fumigation are: (1) Capital investment; (2) Logistics of irradiation processing and fruit transport; and (3) Consumer acceptance. The outlook for radiation disinfestation of Hawaii-grown papaya is quite good in spite of these concerns expressed by the industry. Some packers are beginning to feel that there are more advantages and benefits in adopting this process than the disadvantage of negative publicity about nuclear technology. With the availability of irradiation, the fruit and vegetable industry in Hawaii could be expanded by increased production and sales of new or existing crops. A worthwhile task ahead would be for the food industry, government agencies and researchers to join forces in conducting an effective consumer education programme by assuring the public that irradiated foods are safe. Concurrently, certain segments of the food industry should consider and prepare for the processing and marketing of irradiated foods. (author)

  12. Caracterização anatômica de órgãos vegetativos do mamoeiro Anatomical characterization of vegetative organs of papaya plants

    Directory of Open Access Journals (Sweden)

    Cláudia Elena Carneiro

    2009-06-01

    Full Text Available O mamoeiro (Carica papaya L. é uma planta cuja importância econômica cresceu bastante nos últimos anos, bem como os trabalhos científicos relacionados ao seu crescimento e à sua fisiologia. Infelizmente, foram realizados poucos trabalhos em relação à sua anatomia. Assim, o objetivo deste trabalho é caracterizar anatomicamente plantas do mamoeiro em estádios iniciais do crescimento. Esses estudos são importantes porque podem ser utilizados, principalmente, para correlações com o crescimento e a fisiologia dessa planta. Após coletadas, amostras frescas de raiz, caule e folha foram seccionadas transversalmente à mão livre, e os cortes submetidos ao processo de coloração com safranina e azul de astra. Observou-se que a raiz apresenta estrutura hexarca e caracteriza-se como sendo não axial. A estrutura anatômica do caule, nas regiões mais maduras, assemelha-se à estrutura do pecíolo. A lâmina foliar apresenta epiderme uniestratificada com mesofilo dorsiventral, e os estômatos anomocíticos são observados na face abaxial.Papaya (Carica papaya L. is a plant whose economic importance and scientific studies related to its growth and its physiology have greatly increased in recent years. Unfortunately, few researches in relation to its anatomy were performed. Thus, it was aimed to characterize the anatomically papaya plants in the early stages of growth. These studies are important because they can be used, mainly, for correlations with the growth and the physiology of this plant. After collected, the anatomical cuts of fresh root, stem and leaf were manually performed and their visualization were made through a coloration process using safranine and astra blue. The root structure is hexarc and is characterized as non-axial. The anatomical structure of the stem is similar to petiole in the mature parts. Leaf blade showed an uniestratified epidermis with dorsiventral mesophyll and anomocytic stomata were observed in the leaf abaxial surface.

  13. Dynamic transposable element accumulation in the nascent sex chromosomes of papaya

    OpenAIRE

    VanBuren, Robert; Ming, Ray

    2013-01-01

    From their inception, Y chromosomes in plants and animals are subjected to the powerful effects of Müller’s ratchet, a process spurred by suppression of recombination that results in a rapid accumulation of mutations and repetitive elements. These mutations eventually lead to gene loss and degeneration of the Y chromosome. Y chromosomes in mammals are ancient, whereas most sex chromosomes in plants and many in insects and fish evolved recently. Sex type in papaya is controlled by a pair of na...

  14. Caracterización de diversas fracciones del látex Carica papaya como biocatalizadores en la hidrólisis de triglicéridos

    Directory of Open Access Journals (Sweden)

    Rivera, I.

    2014-03-01

    Full Text Available Carica papaya latex contains interesting enzymes; the best known is papain, but lipolytic activity is also present. Due to the complexity of the latex polymeric matrix, it has not been possible to completely isolate enzymes responsible of lipolytic activity. The aim of this work was to characterize the lipolytic activity in the raw latex (CPLtx and two partially purified fractions of papaya latex (without protease, CPL-p and without esterase CPL-e. Thermostability, optimal temperature and pH in the hydrolysis of two model triglycerides (tributyrin and triolein and the selectivity towards triglycerides with different chain lengths were determined. The lipolytic activity of these biocatalysts in the hydrolysis of tributyrin and olive oil was similar to other commercially available immobilized microbial lipases (RM IM and Novozyme 435.El látex de Carica papaya es fuente de interesantes enzimas, la más conocida es la papaína, pero la actividad lipolítica también está presente. No obstante, debido a la complejidad de la matriz polimérica del látex, hasta la fecha no ha sido posible aislar las enzimas responsables de la actividad lipolítica del látex. Este trabajo está dedicado a la caracterización de la actividad lipolítica en el látex crudo (CPLtx y dos fracciones parcialmente purificadas de látex de papaya (sin proteasas, CPL-p y sin esterasas, CPL-e, a las cuales se determinaron la termostabilidad, temperatura y pH óptimos en la hidrólisis de dos triglicéridos modelo (tributirina y trioleina, así como la selectividad hacia triglicéridos con diferentes longitudes de cadena. Los tres presentaron actividades lipolíticas en hidrólisis de tributirina y aceite de oliva comparables con otras lipasas microbianas inmovilizadas disponibles comercialmente (RM IM y Novozyme 435.

  15. Control of papaya fruits anthracnose by essential oil of Ricinus communis

    OpenAIRE

    César Luis Siqueira Júnior; Maria das Graças Machado Freire; Antônio Sérgio Nascimento Moreira; Maria Ligia Rodrigues Macedo

    2012-01-01

    The aim of this work was to investigate the potential of castor oil for the control of papaya diseases caused by the fungus Colletotrichum gloeosporioides and the bacterium Pseudomonas caricapapayae. The treatment with 1% castor oil did not significantly affect the fungal growth. The effectiveness of castor oil for the control of anthracnose was shown when 5% and 10% (v/v) were used in the assays resulting in reduced mycelial growth. Fungal sporulation was strongly inhibited at 10% (v/v) conc...

  16. Efek Ekstrak Air Buah Pepaya (Carica papaya L. Muda terhadap Gambaran Histologi Kelenjar Mamma Mencit Laktasi

    Directory of Open Access Journals (Sweden)

    Yuktiana Kharisma

    2011-12-01

    Full Text Available Breast milk is the best food for infants. Household Health Survey in 2005 showed exclusive breastfeeding were 4–12% in urban and 4–5% in rural areas. Objective of the study was to examine the effect of unripe papaya aqueous extract (Carica papaya L. on lactating mammary glands histological appearance. The experiment was held on July–September 2009 at Padjadjaran University Clinical Pharmacology Laboratory and Health Research Unit of Dr. Hasan Sadikin Hospital in July–September 2009. A laboratorium experimental study conducted to 21 lactating Swiss Webster mice with 10 babies each. They were divided randomly into 3 groups (n=7. Group I was negative control. Group II: luteotropin 6 mg/30 g BW/day (per oral and group III: unripe papaya aqueous extract 20 mg/30 g BW/day (per oral. Increased milk production was measured by average lactating mammary glands alveolar amounts and diameter count. Experiment started at 4th–16th lactation day. The result was analyzed using analysis of variance (ANOVA followed by Tukey test. The average of alveolar amounts of group I, II and III, were 310.57±30.16, 464.42±25.83, and 465.14±72.41, respectively. The average lactating mamary glands alveolar diameter of group I: 296.50±21.27 ?, group II: 394.57±53.97 ? and group III: 384.29±40.40 ?. Research showed that unripe papaya aqueous extract has better than negative control and equivalent effect with luteotropin on lactating mammary glands histological appearance

  17. Papaya ringspot virus coat protein gene for antigen presentation Escherichia coli.

    Czech Academy of Sciences Publication Activity Database

    Chatchen, S.; Ju?í?ek, Miloslav; Rueda, P.; Kertbundit, Sunee

    2006-01-01

    Ro?. 39, ?. 1 (2006), s. 16-21. ISSN 1225-8687 Grant ostatní: Thai Research Fund(TH) BT-B-06-PG-14-4503 Institutional research plan: CEZ:AV0Z50380511 Source of funding: V - iné verejné zdroje Keywords : antigen presentation * canine parvo virus * epitope * papaya ringspot virus Subject RIV: EF - Botanics Impact factor: 1.465, year: 2006 http://www.jbmb.or.kr/view_article.php3?cont=jbmb&kid=174&mid=3&pid=3

  18. Sensory acceptance of mixed nectar of papaya, passion fruit and acerola

    Directory of Open Access Journals (Sweden)

    Matsuura Fernando César Akira Urbano

    2004-01-01

    Full Text Available Nectars are beverages formulated with the juice or pulp of one or more fruits, plus water and sugar in concentrations resulting in a "ready-to-drink" product. Recently, the market for such products has greatly expanded. Fruit mixtures present a series of advantages, such as the combination of different aromas and flavors and the sum of their nutritional components. The objective of this work was to develop a nectar based on papaya pulp and passion fruit juice, enriched with the vitamin C present in acerola pulp, optimizing the formulation using sensory consumer tests and a response surface statistical methodology. Eleven formulations were prepared using different concentrations of papaya pulp and passion fruit juice and sucrose, and maintaining the concentration of acerola pulp constant. The sensory tests were carried out with 22 non-trained panelists using a structured 9-point hedonic scale to evaluate overall acceptance. The acceptance means were submitted to regression analysis, by first calculating a polynomial quadratic equation. A predictive model was adjusted considering only those parameters where P < 0.05, and a response surface was generated. The overall acceptance of nectars of different formulations varied from 5 ("neither liked nor disliked" to more than 7 ("liked moderately", showing that some products can be considered adequate to consumers, like the nectar produced with 37.5% papaya pulp, 7.5% passion fruit juice, and 5.0% acerola pulp, added of 15% sucrose. A quadratic predictive overall acceptance model, with a regression coefficient of 0.97 was obtained. The sensory acceptance of nectars was positively affected by increases in the concentrations of papaya pulp and of sucrose. Thus, some products presented good sensory acceptance suggesting commercial potential.

  19. Efecto del cloruro de sodio sobre el crecimiento y estado nutricional de plantas de papaya

    Directory of Open Access Journals (Sweden)

    Jorge Parés

    2013-08-01

    Full Text Available La limitada disponibilidad de agua de calidad para la agricultura de las zonas áridas y semiáridas ha propiciado el uso de agua de moderada a alta salinidad. La papaya (Carica papaya L., aunque se reporta con diferentes sensibilidades al estrés salino, es una especie de gran potencial frutícola para ser cultivada económicamente en las regiones semiáridas del estado Lara, Venezuela. El objetivo de este ensayo fue evaluar el efecto de la salinidad ocasionada por el NaCl sobre el crecimiento vegetativo y el estado nutricional de la papaya ‘Maradol’ desarrollada bajo una estructura de techo transparente. Como fuente salina se utilizó cloruro de sodio grado analítico, el cual se adicionó a través del agua de riego en cuatro tratamientos (0,001; 2,0; 4,0 y 8,0 dS·m-1 bajo un diseño completamente aleatorizado con cuatro repeticiones, hasta finalizar la fase juvenil del desarrollo. Se evaluó la longitud del tallo, biomasa seca aérea y radical, área foliar, área de sección del tallo, contenido relativo de clorofila (SPAD y el contenido de N, P, K, Ca, Mg, Mn, Zn, Cu, Fe y Na a nivel foliar. Los tratamientos afectaron el crecimiento vegetativo de las plantas durante toda la fase juvenil del desarrollo. Se observó, además, reducción del contenido de clorofila con el estrés por NaCl. Los tenores N y Na incrementaron, mientras que, los de K, Zn, Cu y Fe disminuyeron. Los niveles de P, Ca, Mg y Mn, no se afectaron. En base a los resultados obtenidos la papaya ‘Maradol’ puede ser considerada como un cultivar de moderada tolerancia al NaCl.

  20. Reasoned opinion on the modification of MRLs for spirodiclofen in strawberries bananas, avocado, mango and papaya

    Directory of Open Access Journals (Sweden)

    European Food Safety Authority

    2012-07-01

    Full Text Available

    In accordance with Article 6 of Regulation (EC No 396/2005, the Netherlands, herewith referred as the evaluating Member State (EMS, received an application from Bayer S.A.S-Bayer CropScience to modify the existing MRLs for spirodiclofen in strawberries and bananas and set import tolerances for papaya, avocado and mango. The Netherlands proposed to decrease the existing MRL for strawberries from 2 mg/kg to 0.02 mg/kg and to increase the MRL for banana from the limit of quantification 0.02* mg/kg to 0.3mg/kg. In order to accommodate the import of produce, the Netherlands proposed to set the MRL for papaya, mangos and avocados at 1.0 mg/kg. The Netherlands drafted an evaluation report according to Article 8 of Regulation (EC No 396/2005, which was submitted to the European Commission and forwarded to EFSA. According to EFSA the data are sufficient to derive MRL proposals of 0.3 mg/kg for the proposed use in banana and 1.5 mg/kg to accommodate the reported use in the USA on avocado, noting that the MRL in the country of origin is set at the level of 1 mg/kg. EFSA has some reservations regarding the proposal that the residue trial results for avocado might be extrapolated to propose MRLs on papaya and mango. The intended use on strawberries is not adequately supported by residue data. Based on the risk assessment results, EFSA concludes that the proposed use of spirodiclofen on strawberry, banana, avocado, mango and papaya will not result in consumer exposure exceeding the toxicological reference value and therefore is unlikely to pose a consumer health risk.

  1. Phenol induced by irradiation does not impair sensory quality of fenugreek and papaya

    Science.gov (United States)

    Chatterjee, Suchandra; Variyar, Prasad S.; Sharma, Arun

    2013-11-01

    The effect of radiation processing on the sensory quality of fenugreek and papaya exposed to doses in the range of 2.5-10 kGy and 100 Gy-2.5 kGy respectively was investigated. Despite an increase in the content of phenol in the volatile oil of these food products overall sensory quality of the irradiated and control samples was not significantly affected by radiation processing.

  2. Contribution of Major Hydrophilic and Lipophilic Antioxidants from Papaya Fruit to Total Antioxidant Capacity

    OpenAIRE

    González-Aguilar, Gustavo A.; Laura E. Gayosso-García Sancho; Yahia, Elhadi M.

    2013-01-01

    Several studies have shown that phenolic and carotenoid compounds protect against oxidative stress, reducing the risk of cardiovascular diseases and some types of cancers. The objective of this research was to determine the individual contribution of the main phenolic compounds from the papaya fruit skin and the individual contribution of main carotenoids from the pulp for total antioxidant capacity at four ripening stages; and the individual and combined radical scavenging ability used the e...

  3. GROWTH OF MACROBRACHIUM ROSENBERGII FED WITH MANGO SEED KERNEL, BANANA PEEL AND PAPAYA PEEL INCORPORATED FEEDS

    OpenAIRE

    P. Aarumugam; P. Saravana Bhavan; T. Muralisankar; Manickam, N; V. Srinevasan; Radhakrishnan, S.

    2013-01-01

    The growth promoting potential of fruits wastes, mango seed kernel, banana peel and papaya peel on the freshwater prawn, Macrobrachium rosenbergii post larvae (PL) was evaluated. Basal diet equated to 35% protein was prepared by using soybean meal, groundnut oilcake, horse gram and wheat flour. Each fruit waste powder was separately incorporated with basal diet at a proportion of 10%. Sunflower oil was used as lipid source. Egg albumin and tapioca flour were used as binding agents. Vitamin B-...

  4. Ethanol Extract of Carica papaya Seeds Induces Reversible Contraception in Adult Male Wistar Rats

    Directory of Open Access Journals (Sweden)

    Wilson O. Hamman

    2011-11-01

    Full Text Available The quest for the development of an ideal male contraceptive has led to the discovery of the antifertility property of some species of C. papaya seed extracts. This study investigates the antifertility activity and reversibility of ethanol extract of C. papaya seeds in sexually matured male Wistar rats. 30 adult males and 60 female Wistar rats weighing between 180 and 220 g were used for the study. The male rats were randomly divided into 3 groups of 10 each. Group I which served as control were orally administered distilled water while groups II and III received 100 and 250 mg/kg/day of ethanol extract of C. papaya seeds respectively for a period of 90 days. 5 males from each group were subjected to fertility test before they were sacrificed to harvest the testes for histopathological analysis. The administration of the extract was discontinued for another 90 days period and the remaining 5 males in each group were subjected to fertility test before they were sacrificed to obtain the testes for histopathological analysis. The results showed normal pregnancy outcome in the females paired with the control group, reduced and zero pregnancy outcome in the females paired with the 100 and 250 mg/kg groups respectively after 90-day administration of the extract. After 90 days of discontinued administration of extract, normal pregnancy outcome were recorded in both the control and treated groups. The result of the histopathological analysis showed a moderate and highly depleted germinal epithelium in the 100 and 250 mg/kg groups respectively after 90 day administration of the extract. The germinal epithelium seen in both the control and the experimental groups were normal after 90 days discontinued extract administration. The study concludes that ethanol extract of C. papaya seeds induces reversible male contraception in Wistar rats.

  5. Establishing Optimal Dehydration Process Parameters for Papaya By EmployingA Firefly Algorithm, Goal Programming Approach

    OpenAIRE

    Julian Scott Yeomans

    2014-01-01

    This study employs a Firefly Algorithm (FA) to determine the optimal osmotic dehydration parameters for papaya. The functional form of the osmotic dehydration model is established via a standard response surface technique. The format of the resulting optimization model to be solved is a non-linear goal programming problem. While various alternate solution approaches are possible, an FA-driven procedure is employed. For optimization purposes, it has been demonstrated that the F...

  6. Caracterização anatômica de órgãos vegetativos do mamoeiro Anatomical characterization of vegetative organs of papaya plants

    OpenAIRE

    Cláudia Elena Carneiro; Jailson Lopes Cruz

    2009-01-01

    O mamoeiro (Carica papaya L.) é uma planta cuja importância econômica cresceu bastante nos últimos anos, bem como os trabalhos científicos relacionados ao seu crescimento e à sua fisiologia. Infelizmente, foram realizados poucos trabalhos em relação à sua anatomia. Assim, o objetivo deste trabalho é caracterizar anatomicamente plantas do mamoeiro em estádios iniciais do crescimento. Esses estudos são importantes porque podem ser utilizados, principalmente, para correlações com o crescimento e...

  7. Studies on moisture sorption isotherms for osmotically dehydrated papaya cubes and verification of selected models

    OpenAIRE

    S. K. Jain (U.S.A.); R. C. VERMA; Sharma, G P; Jain, H.K.

    2010-01-01

    The water desorption properties of osmotically dehydrated papaya cubes at various temperatures were studied by fitting experimental isotherms in Henderson, Oswin, Chen and Clayton and Kuhn equations having 2 parameters and Henderson and Oswin equations were modified to describe the temperature dependence of isotherm data. Oswin equation was useful to predict the equilibrium moisture content values for use in determining the effective moisture diffusion coefficient during subsequent air drying...

  8. Studies on moisture sorption isotherms for osmotically dehydrated papaya cubes and verification of selected models.

    Science.gov (United States)

    Jain, S K; Verma, R C; Sharma, G P; Jain, H K

    2010-06-01

    The water desorption properties of osmotically dehydrated papaya cubes at various temperatures were studied by fitting experimental isotherms in Henderson, Oswin, Chen and Clayton and Kuhn equations having 2 parameters and Henderson and Oswin equations were modified to describe the temperature dependence of isotherm data. Oswin equation was useful to predict the equilibrium moisture content values for use in determining the effective moisture diffusion coefficient during subsequent air drying process. PMID:23572650

  9. Efficiency of a yeast-based formulation for the biocontrol of postharvest anthracnose of papayas

    Directory of Open Access Journals (Sweden)

    Jaqueline Rabelo de Lima

    2014-09-01

    Full Text Available To identify formulations of biological agents that enable survival, stability and a good surface distribution of the antagonistic agent, studies that test different application vehicles are necessary. The efficiency of two killer yeasts, Wickerhamomyces anomalus (strain 422 and Meyerozyma guilliermondii (strain 443, associated with five different application vehicles, was assessed for the protection of postharvest papayas. In this study, after 90 days of incubation at 4ºC, W. anomalus (strain 422 and M. guilliermondii (strain 443 were viable with all application vehicles tested. Fruits treated with different formulations (yeasts + application vehicles had a decreased severity of disease (by at least 30% compared with untreated fruits. The treatment with W. anomalus (strain 422 + 2% starch lowered disease occurrence by 48.3%. The most efficient treatments using M. guilliermondii (strain 443 were those with 2% gelatin or 2% liquid carnauba wax, both of which reduced anthracnose by 50% in postharvest papayas. Electron micrographs of the surface tissues of the treated fruits showed that all application vehicles provided excellent adhesion of the yeast to the surface. Formulations based on starch (2%, gelatin (2% and carnauba wax (2% were the most efficient at controlling fungal diseases in postharvest papayas.

  10. LEAF GAS EXCHANGE CHARACTERISTICS OF FOUR PAPAYA GENOTYPES DURING DIFFERENT STAGES OF DEVELOPMENT

    Directory of Open Access Journals (Sweden)

    CAMPOSTRINI ELIEMAR

    2001-01-01

    Full Text Available In this research, was used four papaya (Carica papaya L. genotypes: three from the 'Solo ( Sunrise Solo TJ, Sunrise Solo 72/12 and Baixinho de Santa Amália group and one from the 'Formosa' group (Know-You 01. They were grown in plastic pots containing a sandy-clay-loam soil subjected to pH correction and fertilization, under greenhouse conditions. Throughout the experimental period plants were subjected to periodic irrigation to maintain the soil humitidy around field capacity. The experiment was conducted 73 days after sowing. In all genotypes, leaf gas exchange characteristics were determined. The net photosynthetic rate (A, mumol m-2 s-1 , stomatal conductance (g s mol m-2 s-1, leaf temperature (T I, 0C and intercellular carbon dioxide concentration (ci, muL L-1 on the 4th, 5th, 6th, 7th, 8th and 9th leaves from the plant apex were determined. No significant differences were observed for A, g s, c i, or Tl either among the leaves sampled from any of the genotypes. A was positively correlated with g s and in the other hand T I and g s were negatively correlated. The results suggest that, for 73 DAP, all the sampled papaya leaves functioned as sources of organs.

  11. Antifungal Activity in Ethanolic Extracts of Carica papaya L. cv. Maradol Leaves and Seeds.

    Science.gov (United States)

    Chávez-Quintal, Pedro; González-Flores, Tania; Rodríguez-Buenfil, Ingrid; Gallegos-Tintoré, Santiago

    2011-01-01

    Bioactive compounds from vegetal sources are a potential source of natural antifungic. An ethanol extraction was used to obtain bioactive compounds from Carica papaya L. cv. Maradol leaves and seeds of discarded ripe and unripe fruit. Both, extraction time and the papaya tissue flour:organic solvent ratio significantly affected yield, with the longest time and highest flour:solvent ratio producing the highest yield. The effect of time on extraction efficiency was confirmed by qualitative identification of the compounds present in the lowest and highest yield extracts. Analysis of the leaf extract with phytochemical tests showed the presence of alkaloids, flavonoids and terpenes. Antifungal effectiveness was determined by challenging the extracts (LE, SRE, SUE) from the best extraction treatment against three phytopathogenic fungi: Rhizopus stolonifer, Fusarium spp. and Colletotrichum gloeosporioides. The leaf extract exhibited the broadest action spectrum. The MIC(50) for the leaf extract was 0.625 mg ml(-1) for Fusarium spp. and >10 mg ml(-1) for C. gloeosporioides, both equal to approximately 20% mycelial growth inhibition. Ethanolic extracts from Carica papaya L. cv. Maradol leaves are a potential source of secondary metabolites with antifungal properties. PMID:22282629

  12. Estimación de las necesidades hídricas de la papaya utilizando la aproximación de los coeficientes culturales duales

    Directory of Open Access Journals (Sweden)

    Yoima Chaterlán

    2012-01-01

    Full Text Available Considerando el incremento de la actividad de riego en el cultivo de la papaya durante los últimos años, resulta de interés el conocimiento de los elementos técnicos y agronomicos necesarios para una adecuada programación del riego a partir de la estimación de las necesidades hidrícas del cultivo. Para esto se utilizo el modelo SIMDualKc el cual efectua el calculo de la evapotranspiración del cultivo (ETc y la programación del riego a partir de la metodología de los coeficientes culturales duales (Kc=Kcb+Ke. La investigación fue realizada en la Estación Experimental de Riego y Drenaje, situada en Alquízar, provincia La Habana, Cuba. Los experimentos fueron desarrollados entre el período marzo-noviembre 1997. Se utilizó la Papaya var. "Maradol roja". La calibración consistió en la búsqueda de los coeficientes culturales basales para las diferentes etapas de desarrollo del cultivo reduciendo al mínimo las diferencias entre los valores simulados y observados del contenido de humedad del suelo. Fue evaluada la bondad de ajuste de las predicciones del modelo, realizándose una serie de análisis estadísticos. Se puede concluir que el modelo puede ser utilizado para la generación de calendarios alternativos de riego destinados a mejorar el ahorro de agua y la productividad de la papaya en estas condiciones edafoclimáticas.

  13. Chemical characterization of the flour of peel and seed from two papaya cultivars

    Scientific Electronic Library Online (English)

    Cláudia Mendes dos, Santos; Celeste Maria Patto de, Abreu; Juliana Mesquita, Freire; Estela de Rezende, Queiroz; Marcelle Mendes, Mendonça.

    2014-06-01

    Full Text Available Papaya is among the currently most important tropical fruits grown in Brazil and in the world. The fruit is mainly consumed fresh although it offers many industrial products. The processing of this fruit, as well as its fresh consumption, results in large amounts of waste, such as peels and seeds. P [...] apaya consumption is one of the causes of significant loss of food value; therefore, new aspects on the use of its waste as by-products, or in the production of food additives, or even the incorporation of its flour in food have aroused great interest because these are products of high nutritional value, and their use may be economically viable. The objective of this study was to produce and characterize peel and seed flours from two papaya cultivars (Havai and Calimosa) for their chemical constituents focusing on possible use in foods. The proximate and mineral composition, titratable acidity, soluble solids, pH, contents of vitamin C, and phenolic compounds were determined. According to the results obtained, the papaya peel and seed flours had high contents of protein and fiber and therefore can be used as alternative sources of nutrients and can also be added in foods avoiding waste and adding value to the fruit.

  14. Relationship between photochemical efficiency (JIP-Test Parameters) and portable chlorophyll meter readings in papaya plants

    Scientific Electronic Library Online (English)

    Fernanda Assumpção, Castro; Eliemar, Campostrini; Alena, Torres Netto; Leandro Hespanhol, Viana.

    Full Text Available The Portable Chlorophyll Meter (PCM) is used to estimate the total chlorophyll and leaf nitrogen content of various species. Since there is a relationship between the chlorophyll concentration and the photochemical efficiency, a relationship can also be established between the PCM readings and some [...] fluorescence variables to provide information on photosystem II (PSII) structure and activity. Papaya (Carica papaya L.) plants ['Sunrise Solo' (leaves with intense green coloring) and 'Golden' cultivars (leaves with yellow-green coloring) were grown in a greenhouse with 30% interception of photosynthetically active photon flux density. The plants were cultivated in 15L plastics pots. Ninety to 100 days after planting the older leaves turned yellow and the chlorophyll fluorescence variables and PCM readings were measured. At low PCM values (less than 30 to 'Sunrise Solo' and less than 20 to 'Golden') the PSII structure and activity were damaged, which is likely related to a reduction in the numbers of active reaction centers (RC/CS0). At higher PCM values the chlorophyll a fluorescence variables (ABS/RC, DI0/RC, TR0/CS0 e ET0/CS0) that are related to PSII structure and activity PSII were not responsive. These results indicated that the effects of chlorophyll content measured by PCM on the photochemical phase of photosynthesis evaluated by chlorophyll fluorescence occur after the degradation of chlorophyll molecules is initiated. In papaya the Portable Chlorophyll Meter (SPAD-502) was shown to be suitable for diagnosis of PSII energy distribution under N stress and senescence.

  15. Color index and correlation with physical and chemical parameters of guava, mango and papaya

    Directory of Open Access Journals (Sweden)

    Jedman Dantas Motta

    2015-03-01

    Full Text Available It was used a low cost colorimeter determine the color in agricultural products. Color analysis on peel and pulps of guava, papaya and mango were made. The values found in the L*a*b* color spaces were used to calculate color indexes that were related to the soluble solids content, pH and the fruit consistence during the maturation. The results obtained permit to conclude that it is possible to estimate the ripening of papaya over the color peel, having found an accurate relation between peel color index and soluble solids content expressed in terms of the third degree equation. Nevertheless for the guava and mango there is not a relation between the peel color indexes and soluble solids content. There is not a relation between color indexes of the guava, papaya and mango pulps with soluble solids content. For all fruits studied there was not relation between color indexes of the peel or pulp with pH.

  16. Isolation of natural inhibitors of papain obtained from Carica papaya latex

    Directory of Open Access Journals (Sweden)

    Rubens Monti

    2004-09-01

    Full Text Available Studies were carried out to natural papain inhibitor from papaya latex. Fresh latex from green fruits of Carica papaya was collected and immediately transported in ice bath to the lab, from which three fractions with inhibitor effect of esterase papain activity were isolated by latex dialysis, Sephadex G-25 gel filtration and ionic exchange chromatography in SP-Sephadex C-25. The isolated fractions, identified as inhibitors I and II, showed a negative reaction with ninhydrin; however, the fraction identified as P-III showed positive reaction with ninhydrin. Kinetics data showed non-competitive inhibition (inhibitor I and uncompetitive (inhibitors II and P-III.Este trabalho apresenta novos dados sobre inibidores naturais de papaína. O látex fresco de frutos verdes de Carica papaya foi coletado pela manhã em plantações da região de Araraquara, SP, Brasil e imediatamente transportado ao laboratório em banho de gelo. Três frações com efeito inibitório da atividade esterásica da papaína foram isoladas a partir do látex fresco, através de diálise, filtração em Sephadex G-25 e cromatografia em SP-Sephadex C-25. As frações isoladas identificadas como inibidores I e II, mostraram reação negativa à ninidrina; entretanto, a fração identificada como P-III mostrou reação positiva. Dados cinéticos revelaram inibição não-competitiva (inibidor I e incompetitiva (inibidores II e P-III.

  17. Isolation of natural inhibitors of papain obtained from Carica papaya latex

    Scientific Electronic Library Online (English)

    Rubens, Monti; Jonas, Contiero; Antonio José, Goulart.

    2004-09-01

    Full Text Available Este trabalho apresenta novos dados sobre inibidores naturais de papaína. O látex fresco de frutos verdes de Carica papaya foi coletado pela manhã em plantações da região de Araraquara, SP, Brasil e imediatamente transportado ao laboratório em banho de gelo. Três frações com efeito inibitório da ati [...] vidade esterásica da papaína foram isoladas a partir do látex fresco, através de diálise, filtração em Sephadex G-25 e cromatografia em SP-Sephadex C-25. As frações isoladas identificadas como inibidores I e II, mostraram reação negativa à ninidrina; entretanto, a fração identificada como P-III mostrou reação positiva. Dados cinéticos revelaram inibição não-competitiva (inibidor I) e incompetitiva (inibidores II e P-III). Abstract in english Studies were carried out to natural papain inhibitor from papaya latex. Fresh latex from green fruits of Carica papaya was collected and immediately transported in ice bath to the lab, from which three fractions with inhibitor effect of esterase papain activity were isolated by latex dialysis, Sepha [...] dex G-25 gel filtration and ionic exchange chromatography in SP-Sephadex C-25. The isolated fractions, identified as inhibitors I and II, showed a negative reaction with ninhydrin; however, the fraction identified as P-III showed positive reaction with ninhydrin. Kinetics data showed non-competitive inhibition (inhibitor I) and uncompetitive (inhibitors II and P-III).

  18. Análisis comparativo y perfil de publicaciones en la Revista Chilena de Pediatría 2001-2006 / Comparative analysis and publication profile in Revista Chilena de Pediatría 2001-2006

    Scientific Electronic Library Online (English)

    PAUL R, HARRIS D; JOSÉ LUIS, LIEBBE G; JONATHAN, SOTOMAYOR A; FRANCISCA, UGARTE P; FRANCISCO, CANO SCH.

    2007-06-01

    Full Text Available La Revista Chilena de Pediatría (Rev Chil Pediatr), publicación oficial de la Sociedad Chilena de Pediatría está indexada en SciELO pero no en la Librería Médica de EEUU (NLM). Objetivos: a) determinar el perfil de los artículos publicados y visitados en la Rev Chil Pediatr, compararlo con el de Jor [...] nal de Pediatría de Brasil (J Ped Br) y el de Journal of Pediatrics (J Ped) de Estados Unidos; b) evaluar la visibilidad de Rev Chil Pediatr y compararla con la Revista Médica de Chile. Métodos: Se evaluaron los ejemplares en línea de las revistas mencionadas a través de la base de datos SciELO y PubMed entre 2001 a 2006. Se tomó una muestra representativa y aleatoria de los artículos publicados en ese período para cada revista. Adicionalmente se evaluaron los artículos más visitados de la Rev Chil Pediatr y se clasificaron como originales (investigación clínica y básica) y no originales (editorial, caso clínico, revisión y otros). Todos los artículos fueron catalogados según su contenido en una de las 25 especialidades médicas definidas. Además se comparó el número de visitas anuales y globales a la Rev Chil Pediatr y a la Revista Médica de Chile y se analizaron las citaciones más frecuentes otorgadas y recibidas por la Rev Chil Pediatr en ese período. Resultados: De 512 artículos en la Rev Chil Pediatr, se analizaron 220; 35% fueron originales y 65% no originales, publicando un porcentaje significativamente menor de artículos originales que el J Ped Br y que el J Ped (p Abstract in english Background: The Revista Chilena de Pediatría (Rev Chil Pediatr), official journal of Sociedad Chilena de Pediatría is index in SciELO, but not in the National Library of Medicine (NLM) of USA. Objectives: a) Determine the profile of published and visited articles of Rev Chil Ped, in order to compare [...] them with the Jornal de Pediatria from Brazil (J Ped Br) and the Journal of Pediatrics (J Ped) from USA. b) Evaluate the visibility of Rev Chil Pediatr compared to Revista Medica de Chile (Rev Med Chil). Method: On line issues from the above mentioned journal were evaluated through SciELO database and PubMed database from 2001 - 2006. A random and representative subset of published articles was obtained from each one of the 3 journals during the referred period. The most visited articles from Rev Chil Pediatr were analyzed and classified as originals (clinical and basic research) and non originals (editorial, clinical case, reviews and others). The articles were classified according to thematic content in 25 medical specialties. In addition, the number of annual visits to Rev Chil Pediatr and Rev Med Chil were analyzed, including the most frequent citations given and received in that period of time. Results: Out of 512 articles in Rev Chil Pediatr, 220 were analyzed; 35% were originals and 65% non originals, with a significant lower percentage of original articles compared to J Ped Br and J Ped (p

  19. Canto para una semilla: Luis Advis, Violeta Parra y la modernización de la música popular chilena

    Scientific Electronic Library Online (English)

    avier, Osorio Fernández.

    2006-06-01

    Full Text Available El objetivo del presente artículo es aportar algunas reflexiones sobre la obra Canto para una semilla del compositor chileno Luis Advis (1972), fundamentalmente a partir de sus dimensiones culturales e históricas. El significado de esta obra es observado en relación con los debates y cuestionamiento [...] s a los que se ve enfrentado el compositor en la sociedad chilena durante las décadas de 1960 y 1970, los cuales se constituyen como expresión de las transformaciones provocadas por la modernización en nuestro país. Esta experiencia concreta de la modernidad se ve reflejada en el contenido poético de las décimas de Violeta Parra, que constituyen el soporte dramático de la obra de Luis Advis, así como también en las vinculaciones entre música culta y música popular, que darán origen posteriormente a la Nueva Canción Chilena y, con ello, redefinirán también el lugar que la música popular posee en nuestra sociedad Abstract in english This article is a study of the piece Canto para una semilla (Song for a Seed) by the Chilean composer Luis Advis (1972), primarily in its cultural and historical dimensions. The meaning of the work is sought in its interrelations with the problems and debates within the Chilean society of the 1960s [...] and 1970s, which were related to the transformations brought about by modernization in Chile. This is mirrored in the poetic content of the décimas written by Violeta Parra, which constitute the dramatic support for Luis Advis's work. This is further related to the connections between art music and popular music which will later originate the Nueva Canción Chilena (New Chilean Song) this redefining the place of popular music in our society

  20. Canto para una semilla: Luis Advis, Violeta Parra y la modernización de la música popular chilena

    Directory of Open Access Journals (Sweden)

    avier Osorio Fernández

    2006-06-01

    Full Text Available El objetivo del presente artículo es aportar algunas reflexiones sobre la obra Canto para una semilla del compositor chileno Luis Advis (1972, fundamentalmente a partir de sus dimensiones culturales e históricas. El significado de esta obra es observado en relación con los debates y cuestionamientos a los que se ve enfrentado el compositor en la sociedad chilena durante las décadas de 1960 y 1970, los cuales se constituyen como expresión de las transformaciones provocadas por la modernización en nuestro país. Esta experiencia concreta de la modernidad se ve reflejada en el contenido poético de las décimas de Violeta Parra, que constituyen el soporte dramático de la obra de Luis Advis, así como también en las vinculaciones entre música culta y música popular, que darán origen posteriormente a la Nueva Canción Chilena y, con ello, redefinirán también el lugar que la música popular posee en nuestra sociedadThis article is a study of the piece Canto para una semilla (Song for a Seed by the Chilean composer Luis Advis (1972, primarily in its cultural and historical dimensions. The meaning of the work is sought in its interrelations with the problems and debates within the Chilean society of the 1960s and 1970s, which were related to the transformations brought about by modernization in Chile. This is mirrored in the poetic content of the décimas written by Violeta Parra, which constitute the dramatic support for Luis Advis's work. This is further related to the connections between art music and popular music which will later originate the Nueva Canción Chilena (New Chilean Song this redefining the place of popular music in our society

  1. Poetas mapuches en la literatura chilena Mapuche poets in Chilean literature

    Directory of Open Access Journals (Sweden)

    Iván Carrasco M

    2000-01-01

    Full Text Available En este trabajo se estudia el modo en que las tradiciones textuales etnoliterarias de los mapuches y literarias de los españoles, se han imbricado en la literatura chilena a través del tiempo, y los aportes que ha dejado la incorporación de poetas de cultura mapuche en la poesía chilena contemporánea. En particular, se establece que autores como Sebastián Queupul, Pedro Alonzo, Elicura Chihuailaf y Leonel Lienlaf han participado en la conformación de la poesía etnocultural, dentro de la cual han propuesto una visión intercultural que sobrepasa los límites de la etnoliteratura mapuche y la literatura chilena tradicional, y un conjunto de estrategias textuales compartidas con otros poetas (enunciación sincrética, intertextualidad transliteraria y codificación plural, en la cual han creado una variedad propia, el doble registro.This paper studies the way in which mapuche textual ethnoliterary traditions and Spanish literary traditions have mixed in the Chilean literature through time, and the contribution of Mapuche poets to contemporary Chilean poetry, particularly, authors such as Sebastián Queupul, Pedro Alonzo, Elicura Chihuailaf and Leonel Lienlaf, where they have created an intercultural view that goes beyond the limits of the mapuche ethnoliterature and Chilean traditional literature, together with a bunch of text strategies shared with others poets (syncretic enunciation, transliterary intertext and plural codification, where they have created their own variety, the double register.

  2. Morfología de la flor y de la semilla de papaya (Carica papaya L.: variedad Maradol e híbrido Tainung-1

    Directory of Open Access Journals (Sweden)

    Gil Arlette Ivonne

    2005-12-01

    Full Text Available

    Con el objetivo de analizar la morfología de la flor y de la semilla de papaya (Carica papaya L. de la variedad ‘Maradol’ y el híbrido ‘Tainung-1’, se recolectó el material vegetal en dos plantaciones y se llevaron al laboratorio de Fisiología de Cultivos, Facultad de Agronomía, Universidad Nacional de Colombia, Bogotá, con el fin de realizar las descripciones correspondientes de los tres tipos de flores (femeninas, hermafroditas y estaminadas y de la semilla, sus características externas (forma, tamaño, hilo, micrópilo, funículo, rafe y cubierta seminal e internas (cubierta seminal, endospermo y embrión. Se concluyó principalmente que a nivel morfológico las flores y semillas de los dos materiales evaluados mostraron características similares, por tanto, las descripciones dadas en este trabajo son generales para ambos.

  3. Gastroprotective and Anti-ulcer activity of Aloe vera juice, Papaya fruit juice and Aloe vera and Papaya fruit combined juice in Ethanol induced Ulcerated Rats

    Directory of Open Access Journals (Sweden)

    S. Gopinathan

    2013-12-01

    Full Text Available Peptic ulcer is the most prevalent gastrointestinal disease. Even though a wide range of drugs are available for the treatment of peptic ulcer, but many of these do not fulfill all the requirements and have side effects. These factors have attracted researchers to investigate the natural products which have more efficacy, less side effects and less expensive for the treatment of peptic ulcer disease. In the present study the anti ulcer activity of (1 Aloe vera juice, (2 papaya fruit juice (3 Aloe vera and papaya fruit combined juice were investigated in the ethanol induced ulcerated rats. The administration of plant juices decreased the offensive factors like ulcer index and acid secretion and also reduced the amount of protein and carbohydrates in the stomach fluid. Further, plant juices increased the defensive factors like activity of oxidative enzymes such as superoxide dismutase and reduced glutathione. Activities of alkaline phosphatase and lipid peroxide were higher in the diseased condition and same were reduced after the treatment with plant juices. Content of haemoglobin and RBC and WBC counts were brought back to normalcy after the treatment with plant juices. The efficacy of plant juices was comparable with the reference drug- Ranitidine. The results of the present study reveal that the plant juices are having efficiency in the gastroprotective activity. It is recommended that the above said plant juices can be further studied for their anti ulcer efficacy in human subjects.

  4. Interculturalidad y ciencias de la educación en lengua de señas chilena

    Directory of Open Access Journals (Sweden)

    Carolina-Alejandra-de-Lourdes Becerra-Sepúlveda

    2013-08-01

    Full Text Available Los paradigmas de intervención orales para la cultura sorda afectan la percepción sobre la lengua de señas Chilena . Esto provoca debates emanados de una subvaloración, dadas sus particularidades visuales: iconicidad e isomorfismo, subvaloración nacida de una incomprensión en la lectura de investigaciones internacionales (por escasez de estudios nacionales, especialmente acerca del lenguaje corporeizado. Esto se contradice con nuevas investigaciones en psicolingüística cognitiva acer-ca de embodied cognition . El abordaje de la LSCh a partir de nuevas teorías atraviesa la interculturalidad y educación a fin de proponerse la metáfora de “la cultura en el cuerpo” como modalidad de estudio contemporáneo.

  5. A physical map of the papaya genome with integrated genetic map and genome sequence

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    Luo Ming-Cheng

    2009-08-01

    Full Text Available Abstract Background Papaya is a major fruit crop in tropical and subtropical regions worldwide and has primitive sex chromosomes controlling sex determination in this trioecious species. The papaya genome was recently sequenced because of its agricultural importance, unique biological features, and successful application of transgenic papaya for resistance to papaya ringspot virus. As a part of the genome sequencing project, we constructed a BAC-based physical map using a high information-content fingerprinting approach to assist whole genome shotgun sequence assembly. Results The physical map consists of 963 contigs, representing 9.4× genome equivalents, and was integrated with the genetic map and genome sequence using BAC end sequences and a sequence-tagged high-density genetic map. The estimated genome coverage of the physical map is about 95.8%, while 72.4% of the genome was aligned to the genetic map. A total of 1,181 high quality overgo (overlapping oligonucleotide probes representing conserved sequences in Arabidopsis and genetically mapped loci in Brassica were anchored on the physical map, which provides a foundation for comparative genomics in the Brassicales. The integrated genetic and physical map aligned with the genome sequence revealed recombination hotspots as well as regions suppressed for recombination across the genome, particularly on the recently evolved sex chromosomes. Suppression of recombination spread to the adjacent region of the male specific region of the Y chromosome (MSY, and recombination rates were recovered gradually and then exceeded the genome average. Recombination hotspots were observed at about 10 Mb away on both sides of the MSY, showing 7-fold increase compared with the genome wide average, demonstrating the dynamics of recombination of the sex chromosomes. Conclusion A BAC-based physical map of papaya was constructed and integrated with the genetic map and genome sequence. The integrated map facilitated the draft genome assembly, and is a valuable resource for comparative genomics and map-based cloning of agronomically and economically important genes and for sex chromosome research.

  6. Efeito do cultivo do mamoeiro (Carica papaya L.) em ambiente protegido sobre a ocorrência de ácaros fitófagos e moscas-brancas / Effect of papaya (Carica papaya L.) cultivated in a protected environment on the occurrence of phytophagous mites and whiteflies

    Scientific Electronic Library Online (English)

    Marineide Rosa, Vieira; Luiz de Souza, Correa; Tatiane Marie Martins Gomes de, Castro; Laura Fernanda Simões da, Silva; Maria de Souza, Monteverde.

    2004-12-01

    Full Text Available O efeito do cultivo do mamoeiro em ambiente protegido foi estudado em três condições: sem cobertura e em dois telados construídos com tela de propileno branca, com malhas de 2 x 2 mm e 2 x 1 mm. Nessa área foram feitas avaliações na cultivar Baixinho de Santa Amália, contando-se o número de plantas [...] com sintomas de ataque recente, para o ácaro branco Polyphagotarsonemus latus, com sintomas e presença de ácaros, para o ácaro rajado Tetranychus urticae e com presença de adultos ou ninfas nas folhas, no caso das moscas-brancas Trialeurodes sp., Bemisia tabaci biótipo B e uma terceira espécie não identificada. Para moscas-brancas, também foram realizadas contagens de ninfas e exúvias em laboratório. O cultivo em ambiente protegido favoreceu a sobrevivência e o desenvolvimento populacional das espécies estudadas, sendo que algumas possíveis causas são discutidas no texto. Considerando-se que o cultivo protegido pode ser uma boa alternativa para o controle de viroses, como o mosaico do mamoeiro, problema limitante para a cultura, estratégias de manejo de pragas nesses ambientes devem ser desenvolvidas, para viabilizar o seu uso. Abstract in english It was measured the effect of cultivating papaya cultivar "Baixinho de Santa Amália" in a protected environment and in three situations: without shade, and under screen shades of white polyethylene mesh sizes 2 x 2 mm or 2 x 1 mm. It was registered the number of plants with symptoms of recent attack [...] s of the broad mite (Polyphagotarsonemus latus), symptoms and presence of the two-spotted spider mite (Tetranychus urticae) and the presence on leaves of adults or nymphs of whiteflies (Trialeurodes sp., Bemisia tabaci biotype B and a third unidentified species). Whitefly nymph and exuviae numbers were also counted in the laboratory. Cultivation in a protected environment favored the survival and development of the species under study, and some possible reasons for these are discussed along the text. Since cultivating papaya in protected environment can be a good alternative for "papaya ringspot virus" control, which is a restrictive factor for the crop, the development of strategies to control the pests in this condition is necessary for correct recommendation of such measure.

  7. Efeito do cultivo do mamoeiro (Carica papaya L. em ambiente protegido sobre a ocorrência de ácaros fitófagos e moscas-brancas Effect of papaya (Carica papaya L. cultivated in a protected environment on the occurrence of phytophagous mites and whiteflies

    Directory of Open Access Journals (Sweden)

    Marineide Rosa Vieira

    2004-12-01

    Full Text Available O efeito do cultivo do mamoeiro em ambiente protegido foi estudado em três condições: sem cobertura e em dois telados construídos com tela de propileno branca, com malhas de 2 x 2 mm e 2 x 1 mm. Nessa área foram feitas avaliações na cultivar Baixinho de Santa Amália, contando-se o número de plantas com sintomas de ataque recente, para o ácaro branco Polyphagotarsonemus latus, com sintomas e presença de ácaros, para o ácaro rajado Tetranychus urticae e com presença de adultos ou ninfas nas folhas, no caso das moscas-brancas Trialeurodes sp., Bemisia tabaci biótipo B e uma terceira espécie não identificada. Para moscas-brancas, também foram realizadas contagens de ninfas e exúvias em laboratório. O cultivo em ambiente protegido favoreceu a sobrevivência e o desenvolvimento populacional das espécies estudadas, sendo que algumas possíveis causas são discutidas no texto. Considerando-se que o cultivo protegido pode ser uma boa alternativa para o controle de viroses, como o mosaico do mamoeiro, problema limitante para a cultura, estratégias de manejo de pragas nesses ambientes devem ser desenvolvidas, para viabilizar o seu uso.It was measured the effect of cultivating papaya cultivar "Baixinho de Santa Amália" in a protected environment and in three situations: without shade, and under screen shades of white polyethylene mesh sizes 2 x 2 mm or 2 x 1 mm. It was registered the number of plants with symptoms of recent attacks of the broad mite (Polyphagotarsonemus latus, symptoms and presence of the two-spotted spider mite (Tetranychus urticae and the presence on leaves of adults or nymphs of whiteflies (Trialeurodes sp., Bemisia tabaci biotype B and a third unidentified species. Whitefly nymph and exuviae numbers were also counted in the laboratory. Cultivation in a protected environment favored the survival and development of the species under study, and some possible reasons for these are discussed along the text. Since cultivating papaya in protected environment can be a good alternative for "papaya ringspot virus" control, which is a restrictive factor for the crop, the development of strategies to control the pests in this condition is necessary for correct recommendation of such measure.

  8. Teste de condutividade de elétrica para avaliação da qualidade fisiológica de sementes de mamão (Carica papaya L.) / Electrical conductivity test for physiological quality evaluation of papaya seeds (Carica papaya L.)

    Scientific Electronic Library Online (English)

    Dai, Tokuhisa; Camilla Atsumi Zanuncio, Sediyama; Paulo César, Hilst; Denise Cunha Fernandes dos Santos, Dias.

    Full Text Available O trabalho teve como objetivo verificar a eficiência do teste de condutividade elétrica para avaliar a qualidade fisiológica de sementes de mamão. Foram utilizados cinco lotes do híbrido Tainung 01, determinando-se, inicialmente, o teor de água das sementes. Em seguida, as sementes foram submetidas [...] aos testes de germinação, envelhecimento acelerado, índice de velocidade de emergência de plântulas e massa seca de plântula. O teste de condutividade elétrica foi conduzido com quatro repetições de 50 sementes, utilizando-se duas temperaturas (25ºC e 30ºC), dois volumes de água (50 e 75mL) e seis períodos de embebição (2, 4, 6, 8, 24 e 48 horas). Verificou-se que a condutividade elétrica aumentou com a elevação da temperatura e do período de embebição e decresceu com o aumento do volume de água. O teste de condutividade é eficiente para detectar diferenças na qualidade fisiológica dos lotes de sementes de mamão, especialmente quando as sementes são imersas em 50mL de água, a 25ºC, ou em 75mL de água, a 30ºC . Nestas condições, o período de embebição das sementes pode ser reduzido para duas horas. Abstract in english The objective of this study was to verify the efficiency of the electrical conductivity test for physiological quality evaluation of papaya (Carica papaya L.) seeds. Initially, seeds from five lots, Tainung 01 hybrid, were submitted to the following tests: seed moisture content, standard germination [...] , first count, accelerated aging, percentage and speed of seedling emergence index and seedling dry weight. Electrical conductivity tests were performed using four replications of 50 seeds, two temperatures (25 and 30ºC), two water volumes (50 and 75mL) and six soaking periods (2, 4, 6, 8, 24 and 48 hours). It was observed that the electrical conductivity increased with the rise of temperature and soaking period but decreased with the increase in water volume. This test was efficient to detect differences in physiological quality of papaya seed lots, especially when the seeds were soaked in 50mL water at 25ºC, or in 75mL water at 30ºC. Under these conditions, the seed soaking period can be reduced to two hours.

  9. DNA extraction method using a silica-base resin type kit for the detection of genetically modified papaya.

    Science.gov (United States)

    Ohmori, Kiyomi; Tsuchiya, Hisayo; Watanabe, Takahiro; Akiyama, Hiroshi; Maitani, Tamio; Yamada, Toshiharu; Hirayama, Kuni; Satoh, Shuji

    2008-04-01

    Genetically modified (GM) papaya has not yet been approved for importation into, or cultivation in the European Union (EU) and Japan. A DNA extraction method using the Qiagen DNeasy Plant Mini Kit (PM method) and a method using a buffer containing cetyltrimethyl ammonium bromide (CTAB method) have been adopted as the official Japanese methods for detecting GM foods. However, the amounts of DNA extracted from papaya by these methods are very low. Therefore, we investigated an extraction method to obtain a high yield of DNA from raw or freeze-dried fresh papaya using the Promega Wizard DNA Clean-Up Resin System (WCR). The incubation for the extraction was carried out at 58 degrees C without proteinase K for 15 min. The extract was applied to a mini-column, then the column was washed with 80% isopropyl alcohol, and genomic DNA adsorbed on the column was eluted with TE buffer. The WCR method gave a higher yield of genomic DNA, and was simpler and faster than the PM method or CTAB method. In addition, it could be used to extract genomic DNA from fresh papaya at various stages of ripeness. Based on these results, we propose that the present method using WCR is the most practical and useful way to extract genomic DNA for the purpose of detecting GM papaya. PMID:18503240

  10. Repeated Dose 28-Days Oral Toxicity Study of Carica papaya L. Leaf Extract in Sprague Dawley Rats

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    Hussin Muhammad

    2012-04-01

    Full Text Available Carica papaya L. leaves have been used in ethnomedicine for the treatment of fevers and cancers. Despite its benefits, very few studies on their potential toxicity have been described. The aim of the present study was to characterize the chemical composition of the leaf extract from ‘Sekaki’ C. papaya cultivar by UPLC-TripleTOF-ESI-MS and to investigate the sub-acute oral toxicity in Sprague Dawley rats at doses of 0.01, 0.14 and 2 g/kg by examining the general behavior, clinical signs, hematological parameters, serum biochemistry and histopathology changes. A total of twelve compounds consisting of one piperidine alkaloid, two organic acids, six malic acid derivatives, and four flavonol glycosides were characterized or tentatively identified in the C. papaya leaf extract. In the sub-acute study, the C. papaya extract did not cause mortality nor were treatment-related changes in body weight, food intake, water level, and hematological parameters observed between treatment and control groups. Some biochemical parameters such as the total protein, HDL-cholesterol, AST, ALT and ALP were elevated in a non-dose dependent manner. Histopathological examination of all organs including liver did not reveal morphological alteration. Other parameters showed non-significant differences between treatment and control groups. The present results suggest that C. papaya leaf extract at a dose up to fourteen times the levels employed in practical use in traditional medicine in Malaysia could be considered safe as a medicinal agent.

  11. Low-dose gamma irradiation following hot Water immersion of Papaya (Carica Papaya linn.) fruits provides additional control of postharvest fungal infection to extend shelf life

    DEFF Research Database (Denmark)

    Rashid, M.H.A.; Grout, Brian William Wilson; Continella, A.; Mahmud, T.M.M.

    2015-01-01

    Low-dose gamma irradiation (0.08 kGy over 10 min), a level significantly below that required to satisfy the majority of international quarantine regulations, has been employed to provide a significant reduction in visible fungal infection on papaya fruit surfaces. This is appropriate for local and...... national markets in producer countries where levels of commercial acceptability can be retained despite surface lesions due to fungal infection. Irradiation alone and in combination with hot-water immersion (50 °C for 10 min) has been applied to papaya (Carica papaya L.) fruits at both the mature green and...... combined treatment had no significant, negative impact on ripening, with quality characteristics such as surface and internal colour change, firmness, soluble solids, acidity and vitamin C maintained at acceptable levels....

  12. Etiología y epidemiología de la necrosis de flores y frutos juveniles del papayo (Carica papaya L.) en Guerrero, México / Etiology and epidemiology of flowers and young fruits necrosis in papaya (Carica papaya L.) in Guerrero, México

    Scientific Electronic Library Online (English)

    Alfonso, Vásquez-López; Elías, Hernández-Castro; J. Antonio, Mora-Aguilera; Cristian, Nava-Díaz; Francisco, Sánchez-García.

    2012-12-01

    Full Text Available La necrosis floral, pudrición y caída de frutos juveniles del papayo (Caricapapaya L.) son enfermedades con incidencia alta y reducen la productividad de huertos comerciales en Huamuxtitlán, Guerrero, México. El objetivo del presente estudio fue determinar la etiología y epidemiología de estas enfer [...] medades. De flores y frutos necróticos se aislaron Cladosporium sp. y Fusarium spp., y en proporción menor Alternaria sp. y Colletotrichum sp. Las especies Fusarium equiseti, F. chlamydosporum, Cladosporium cladosporioides y Alternaria alternata provocaron necrosis y caída floral 8 d después de la inoculación (ddi) y necrosis, pudrición y caída de frutos juveniles 15 ddi. Colletotrichum gloeosporioides provocó lesiones necróticas en 30 % de las flores inoculadas y no causó síntomas en frutos. La dinámica poblacional de esporas de Alternaria spp. y Fusarium spp. en el aire se estudió en una plantación de papayo cv. Maradol de marzo del 2007 a marzo del 2008; las esporas de ambos hongos se encontraron de mayo a octubre, aunque el número mayor se capturó en mayo y septiembre. Las esporas de Fusarium spp. fueron más abundantes en horario nocturno (20:00 a 04:00 h) y las de Alternaria spp. entre las 12:00 y 14:00 h. Fusarium equiseti, F. chlamydosporum, Cladosporium cladosporioides y Alternaria alternata son agentes patógenos de la necrosis de lores y pudrición y caída de frutos juveniles del papayo en Huamuxtitlán, Guerrero, México. Los propágulos de Alternaria spp. y Fusarium spp. están dispersos en el ambiente de la zona de estudio de mayo a octubre. Abstract in english Flower necrosis, rot, and dropping of young fruits of the papaya (Carica papaya L.) are diseases with high incidence and which reduce productivity of commercial groves in Huamuxtitlán, Guerrero, Mexico. The objective of the present study was to determine the etiology and epidemiology of these diseas [...] es. Cladosporium sp. and Fusarium spp. were isolated from necrotic flowers and fruits, and in a smaller proportion, Alternaria sp. and Colletotrichum spp. The species Fusarium equiseti, F. chlamydosporum, Cladosporium cladosporoides and Alternaria alternate caused necrosis and flower abortion 8 d after inoculation (dai) and necrosis, rot and dropping of young fruits 15 dai. Colletotrichum gloeosporioides provoked necrotic lesions in 30 % of the inoculated flowers but did not cause symptoms in fruits. The population dynamic of spores of Alternaria spp. and Fusarium spp. in the air was studied in a plantation of papaya cv. Maradol from March 2007 to March 2008; the spores of both fungi were found from May to October, although the highest number was captured in May and September. The spores of Fusarium spp. were most abundant during the night hours (20:00 to 04:00 h) and those of Alternaria spp. between 12:00 and 14:00 h. Fusarium equiseti, F. chlamydosporum, Cladosporium cladosporioides and Alternaria alternata are pathogenic agents of the necrosis of flowers and dropping of young fruits of papaya in Huamuxtitlán, Guerrero, México. The propagules of Alternaria spp. and Fusarium spp. are dispersed in the environment of the study zone from May to October.

  13. Study about papaya (Carica papaya L.) conservation submitted to a combined treatment of irradiation and an edible film of quitosan; Estudo do tratamento combinado de radiacao ionizante e cobertura de quitosana em mamao papaia (Carica papaya L.)

    Energy Technology Data Exchange (ETDEWEB)

    Camargo, Rita Junqueira de

    2004-07-01

    In this research, on a first stage the effect of two treatments were studied: thermal, and a chitosan edible coating, combined with four different irradiation doses: 0,00 kGy, 0,50 kGy, 0,75 kGy and 1,00 kGy for effects in the ripening of papaya (Carica Papaya) cv. Golden. To evaluate the influence of these two factors in the physical-chemical characteristics of the fruit during ripening, analysis on color, both skin and pulp, firmness, soluble solids, pH and titrable acidity were performed. The incidence of fungi in fruit skin was also analyzed. The fruits were first maintained at 10 deg C chamber for fifteen days to simulate maritime transportation to the importer country and then transferred to a 25 deg C chamber aiming simulation of the commercialization conditions. From this first step, the conclusion was that chitosan in the concentration of 2% was not able to contain the fungi development and that it also influenced in a significant form the variable pH. A more expressive result was obtained for irradiation doses of 0,75 kGy and 1,00 kGy, as it was observed in the beginning the acceleration in ripening through the variables skin color and penetration energy of the fruit pulp. When storage temperature was changed to 25 deg C this situation inverted for these same variables and a two day delay was observed on the development of yellow color on the fruit skin and loss of pulp firmness. The variables titrable acidity, soluble solids and pulp color were not influenced by treatment or by irradiation. On a second stage, the fruits, in the same condition of the previous stage, were analyzed in relation of their mass and ripening stage using a ripening scale provided by the producer of the fruits for this experiment, as to evaluate the gradual development of fruit ripening according to the various treatments (chitosan 3%, thermal or control) and irradiation dose (0,00 kGy and 0,75 kGy). Confirming the results obtained in the first stage, the irradiation was able to delay ripening in one day and a half approximately. The chitosan in these new conditions of test propitiated fungi development and the fruits coated with chitosan did not ripe in the way it was seen to come. A last stage was done to verify consumer behavior in respect to an irradiated papaya. By three different sensorial tests it was verified if provers were able to perceive significant difference between irradiated and non irradiated samples, what was the acceptance of a irradiated sample and the intention of purchase of a irradiated and a non irradiated papaya. Conclusion from these tests was that the difference established in the triangular test was not enough to establish significant difference between the acceptances of the two samples in the four parameters tested: appearance, texture, aroma and flavor. For the purchase intention it was verified that the irradiation is not a barrier for the consumer. (author)

  14. Penurunan Jumlah dan Motilitas Spermatozoa Setelah Pemberian Ekstrak Biji Pepaya (Kajian Potensi Biji Papaya sebagai Bahan Kontrasepsi Alternatif The Declining of Spermatozoa Number and Motility of Mice Were Treats with Papaya Seeds Extract

    Directory of Open Access Journals (Sweden)

    Wulan Kristijanti

    2011-04-01

    Full Text Available Organs of many plants have been used as male contraception materials. One of the organs is papaya seed. This seed contains carpain, a molecule that could cause vacuole initiation on Sertoli cell. The research aimed to know whether papaya seed carpain affects mice spermatozoa quality. This research is an experimental one that makes use of 24 male mice. These mice are separated into 4 groups. Each group has its own papaya seed extract doses. The first group is the mice group that is given by doses of 0 mg/200 gr body weights. And the following groups are 10 mg/200 gr body weight for group II, 20 mg/200 gr body weight for group III, and 40 mg/200 gr body weight for group IV. Oral supplement of the papaya seed is 1 ml/mouse/day in the term of 40 days. These oral supplement is followed by reversibility time for 40 days. The data consist of spermatozoa viability, motility, and amount. These data are analyzed by 2 x 4 factorial analysis in the test scale of 5%. Statistical analysis shows significant differences among each treatment (8.27 > 2.76, spermatozoa taking time (5.81 > 4.60 and dose variation among groups (15.29 > 3.34. There are also significant differences among motility treatment (24.94 > 2.76, time (4.66 > 4.60 and doses (55.03 > 3.34. There is no significant difference among viability (3.22 < 3.74 nor treatment (2.30 < 2.76. The research concluded that papaya seed extract affects the spermatozoa motility and the decrease of spermatozoa numbers, but not to the reversibility and the viability. Keywords: contraception, papaya seed, reversibility

  15. New records of Discomycetes (Pezizales for the Chilean mycobiota Nuevos registros de Discomycetes (Pezizales para la micobiota chilena

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    PABLO SANDOVAL

    2012-01-01

    Full Text Available Se adicionan a la micobiota chilena tres especies de Pezizales, Byssonectria terrestris (Alb. & Schwein. Pfister, Pseudoplectania nigrella (Pers. Fuckel y Pseudoplectania sphagnophila (Pers. Kreisel, Se describen e ilustran los caracteres diagnósticos y se realizan comentarios acerca de las especies.

  16. Control of egg hatch ability and adult emergence of three fruit fly species in papayas by gamma irradiation

    International Nuclear Information System (INIS)

    The effects of gamma radiation on the oriental fruit fly, Bactrocera Dorsalis (Hendel), melon fly, Bactrocera Cucurbitae (Coquilett), and Mediterranean fruit fly, Ceratitis Capitata (Weidemann) were studied. Melon fly was determined to be the most susceptible of the three species. A dosage of 550 Gy rendered the eggs 100% sterile when irradiated in papayas at 4-6 hours before hatching. Oriental and mediterranean fruit flies were found to be more resistant, requiring doses of 750 and 850 Gy, respectively. A dose of only 100 Gy was needed to inhibit adult eclosion when the three species were treated at third instar larvae. Warm water treatment at 490C for 20 minutes was found sufficient in preventing the hatching of any egg in the infested papaya fruits. However, since eggs may hatch before the warm-water treatment can be applied, a combination of irradiation treatment using 100 Gy is recommended for disinfestation of papaya fruits. (author). 17 refs.; 3 tabs

  17. DEL MALTRATO AL UXORICIDIO. LA VIOLENCIA «PUERTAS ADENTRO» EN LA ALDEA CHILENA TRADICIONAL (Siglo XIX

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    René Salinas Meza

    2011-10-01

    Full Text Available Este artículo pone de manifiesto el contexto en el cual las mujeres chilenas de las aldeas del siglo XIX son víctimas de diversos tipos de agresiones físicas, directas e indirectas, y psicológicas. Las conductas violentas de los agresores son explicadas en el marco de una comunidad que las reconoce como un componente habitual de la vida cotidiana. Así, se delimitan espacios específicos en los que se desenvuelven las relaciones de la gente, tales como el mundo urbano de la aldea y el mundo doméstico de la casa. En ambos, las personas construyen redes colectivas de solidaridad y fraternidad; pero, al mismo tiempo, desarrollan relaciones de competencia que son generadoras de roces y agresiones que ilustran la sociabilidad y el parentesco. Por otra parte, las mujeres agredidas confundieron a menudo la agresión con la aceptación de la autoridad masculina que permitía suponer la existencia de un matrimonio socialmente aceptado y cotidianamente bien llevado. La actitud de rebeldía femenina chocaba con el rechazo social y el ejercicio de un poder marital que se expresaba en conductas agresivas. Así, las relaciones en la pareja no fueron entre iguales, sino jerárquicas, imponiendo a las mujeres un rol subordinado y dependiente frente a los hombres, los que, a su vez, ostentaban la autoridad suficiente para controlarlas y castigarlas. Con la ayuda documental de un abundante número de expedientes judiciales se intenta comprender y explicar las conductas que asumen hombres y mujeres en la relación familiar desarrollada en las aldeas chilenas del siglo XIX.

  18. New approach for papaya latex storage without virus degradation Nova metodologia de armazenamento do latex de mamão sem degradação viral

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    Silas P. Rodrigues

    2009-03-01

    Full Text Available Papaya meleira virus (PMeV is the causal agent of papaya (Carica papaya L. sticky disease, which has been detected through analysis of its double-stranded RNA (dsRNA genome from plant latex. In this work we demonstrate that PMeV dsRNA is protected during 25 days when latex is diluted in citrate buffer pH 5.0 (1:1 v/v and maintained at -20°C. At the same temperature, some protection was observed for pure latex or latex diluted in ultra-pure water. Conversely, the dsRNA was almost completely degraded after 25 days when maintained at 25°C, indicating the need for freezing. The proper procedures to collect and store papaya latex described here will contribute to efficient and large scale use of molecular diagnosis of PMeV.Papaya meleira virus (PMeV é o agente etiológico da meleira do mamoeiro (Carica papaya L., cujo diagnóstico é feito através da detecção do RNA dupla-fita (dsRNA viral a partir do látex das plantas. Neste trabalho é demonstrado que o dsRNA do PMeV é protegido durante 25 dias quando diluído em tampão citrato pH 5.0 (1:1 v/v seguido de armazenamento à -20°C. Nesta mesma temperatura, o dsRNA foi parcialmente protegido quando o látex foi diluído em água ultra-pura ou mantido puro. Ao contrário, quando as amostras foram mantidas à 25°C, observou-se uma degradação progressiva do dsRNA, com ausência de bandas após 25 dias, indicando a necessidade do congelamento do látex. Os procedimentos de coleta e armazenamento do látex descritos neste trabalho contribuem para a eficiência e uso em larga escala do diagnóstico molecular do PMeV.

  19. Effect of irradiation on the biochemical and organoleptic changes during the ripening of papaya and mango fruits

    Energy Technology Data Exchange (ETDEWEB)

    Lacroix, M.; Bernard, L.; Jobin, M.; Milot, S.; Gagnon, M. (Centre d' Irradiation du Canada, Laval, Quebec (Canada))

    1990-01-01

    Papaya and mango rot caused by fungi is a major problem during storage and marketing. Gamma irradiation treatment was used to determine its effect on the quality of papayas and mangoes irradiated at 0,5 to 0,95 kGy. The level of respiration, soluble solids, texture, vitamin C and the sensorial evaluation were effectuated. The results indicate that irradiation treatment reduces significantly (p{le}0,001) weakens the texture of mangoes. The content of soluble solids and vitamin C are not significantly affected by the irradiation. The sensory evaluation indicates that up to 0,95 kGy the sensorial quality is not changed. (author).

  20. Repeated Dose 28-Days Oral Toxicity Study of Carica papaya L. Leaf Extract in Sprague Dawley Rats

    OpenAIRE

    Hussin Muhammad; Ibrahim Jantan; Noordini Abdullah; Raja Hazlini Raja Semail; Badrul Amini Rashid; Siti Zaleha Halim; Noor Rain Abdullah; Adlin Afzan; Zakiah Ismail

    2012-01-01

    Carica papaya L. leaves have been used in ethnomedicine for the treatment of fevers and cancers. Despite its benefits, very few studies on their potential toxicity have been described. The aim of the present study was to characterize the chemical composition of the leaf extract from ‘Sekaki’ C. papaya cultivar by UPLC-TripleTOF-ESI-MS and to investigate the sub-acute oral toxicity in Sprague Dawley rats at doses of 0.01, 0.14 and 2 g/kg by examining the general behavior, clinical signs, hemat...

  1. Effect of irradiation on the biochemical and organoleptic changes during the ripening of papaya and mango fruits

    International Nuclear Information System (INIS)

    Papaya and mango rot caused by fungi is a major problem during storage and marketing. Gamma irradiation treatment was used to determine its effect on the quality of papayas and mangoes irradiated at 0,5 to 0,95 kGy. The level of respiration, soluble solids, texture, vitamin C and the sensorial evaluation were effectuated. The results indicate that irradiation treatment reduces significantly (p?0,001) weakens the texture of mangoes. The content of soluble solids and vitamin C are not significantly affected by the irradiation. The sensory evaluation indicates that up to 0,95 kGy the sensorial quality is not changed. (author)

  2. Una recepción diferente: La arquitectura moderna brasileña y la cultura arquitectónica chilena A Diferent Reception: Modern Brazilian Architecture and Chilean Architectonic Culture

    Directory of Open Access Journals (Sweden)

    Horacio Torrent

    2011-08-01

    Full Text Available La difusión de la arquitectura brasileña hacia fines de los cuarenta construyó un discurso que, preferentemente, se detenía en la exuberancia y la libertad formales; las publicaciones chilenas de la época miraban, sin embargo, en otra dirección.

  3. Una recepción diferente: La arquitectura moderna brasileña y la cultura arquitectónica chilena / A Diferent Reception: Modern Brazilian Architecture and Chilean Architectonic Culture

    Scientific Electronic Library Online (English)

    Horacio, Torrent.

    2011-08-01

    Full Text Available La difusión de la arquitectura brasileña hacia fines de los cuarenta construyó un discurso que, preferentemente, se detenía en la exuberancia y la libertad formales; las publicaciones chilenas de la época miraban, sin embargo, en otra dirección. [...

  4. Determinação de isotiocianato de benzila em Carica papaya utilizando cromatografia gasosa com detectores seletivos / Determination of benzyl isothiocyanate in Carica papaya using gas chromatography with selectives detectors

    Scientific Electronic Library Online (English)

    Izabela Miranda de, Castro; Marianna Ramos dos, Anjos; Elba dos Santos de, Oliveira.

    Full Text Available [...] Abstract in english In the present work, a method was developed and validated for the quantification of benzyl isothiocyanate (BITC) in the fruits of Carica papaya. The quantification of this compound was carried out by gas chromatography (GC) with selective detectors - nitrogen phosphorus detector (NPD) and flame phot [...] ometric detector (FPD). The performance of these detectors showed a higher sensitivity of the NPD with a broader linear range of detection. The LOD/LOQ were 0.038/0.100 µg/mL for NPD and 5.78/19.29 µg/mL for FPD. The recovery of the method for BITC was 90,64%. An average value of BITC concentration in all the analyzed samples was 16,23 µg BITC/g.

  5. Determinação de isotiocianato de benzila em Carica papaya utilizando cromatografia gasosa com detectores seletivos Determination of benzyl isothiocyanate in Carica papaya using gas chromatography with selectives detectors

    Directory of Open Access Journals (Sweden)

    Izabela Miranda de Castro

    2008-01-01

    Full Text Available In the present work, a method was developed and validated for the quantification of benzyl isothiocyanate (BITC in the fruits of Carica papaya. The quantification of this compound was carried out by gas chromatography (GC with selective detectors - nitrogen phosphorus detector (NPD and flame photometric detector (FPD. The performance of these detectors showed a higher sensitivity of the NPD with a broader linear range of detection. The LOD/LOQ were 0.038/0.100 µg/mL for NPD and 5.78/19.29 µg/mL for FPD. The recovery of the method for BITC was 90,64%. An average value of BITC concentration in all the analyzed samples was 16,23 µg BITC/g.

  6. Métodos de extracción de semilla en papaya Golden y la relación con la longevidad Extraction methods in Golden type papaya seed and relationship with longevity

    Directory of Open Access Journals (Sweden)

    Julio César García Dean

    2011-04-01

    Full Text Available Es muy escasa la información disponible, relacionada al manejo de poscosecha de semilla de papaya (Carica papaya L. y su influencia en la calidad fisiológica de la semilla, sobre todo en germinación y vigor. El trabajo de investigación se realizó en el laboratorio de producción de semillas del Centro de Capacitación y Desarrollo de Tecnología de Semillas, en el invernadero número uno de la Universidad Autónoma Agraria "Antonio Narro" (UAAAN durante 2008. La finalidad del trabajo fue evaluar seis tratamientos de extracción de semilla: se utilizó ácido clorhídrico (HCl a 0.3 y 0.5% durante una hora de reposo, ácido sulfúrico (H2SO4 a 0.3% a 1.5 h de reposo, hipoclorito de sodio (NaClO al 5% con media hora de reposo y fermentación a 48 y 72 h. La semilla extraída fue almacenada durante 90, 180 y 240 días a 4 °C. Se evaluó la germinación y vigor de la semilla. Se utilizó un diseño completamente al azar con arreglo factorial. Los resultados indicaron que la calidad fisiológica de la semilla, fue afectada por los efectos de deterioro que causa el almacenamiento y por los métodos de extracción de semilla; sin embargo, la semilla conserva su calidad fisiológica hasta los 240 días de almacenamiento, en los tratamientos a base de HCl al 0.3% y fermentación por 24 h con 93.3 y 91.2% de germinación después de 240 días de almacenamiento.It is very scarce available information related to post-harvest handling of papaya seed (Carica papaya L. and their influence in seed physiologic quality, mainly in germination and vigor. The investigation work was carried out in seeds production laboratory of Center of Training and Development of Seeds Technology, in greenhouse number one of Universidad Autónoma Agraria "Antonio Narro" (UAAAN during 2008. Aim of work was to evaluate six treatments of seed extraction: hydrochloric acid was used (HCl at 0.3 and 0.5% during one hour of rest, sulfuric acid (H2SO4 at 0.3% with 1.5 h of rest, sodium hypochlorite (NaClO at 5% with 30 minutes of rest and fermentation at 48 and 72 h. Extracted seed was stored during 90, 180 and 240 days at 4 °C. It was evaluated seed germination and vigor. A totally random design was used with factorial arrangement. Results indicated that seed physiologic quality was affected by deterioration effects caused by storage and by methods of seed extraction; however, seed conserves its physiologic quality up to 240 days of storage, in treatments with help of HCl at 0.3% and fermentation for 24 h with 93.3 and 91.2 % germination after 240 days of storage.

  7. Métodos de extracción de semilla en papaya Golden y la relación con la longevidad / Extraction methods in Golden type papaya seed and relationship with longevity

    Scientific Electronic Library Online (English)

    Julio César, García Dean; Mario Ernesto, Vázquez Badillo; María Alejandra, Torres Tapia; Sergio Ignacio, Dávila Cabello; David, Sánchez Aspeytia.

    2011-04-01

    Full Text Available Es muy escasa la información disponible, relacionada al manejo de poscosecha de semilla de papaya (Carica papaya L.) y su influencia en la calidad fisiológica de la semilla, sobre todo en germinación y vigor. El trabajo de investigación se realizó en el laboratorio de producción de semillas del Cent [...] ro de Capacitación y Desarrollo de Tecnología de Semillas, en el invernadero número uno de la Universidad Autónoma Agraria "Antonio Narro" (UAAAN) durante 2008. La finalidad del trabajo fue evaluar seis tratamientos de extracción de semilla: se utilizó ácido clorhídrico (HCl) a 0.3 y 0.5% durante una hora de reposo, ácido sulfúrico (H2SO4) a 0.3% a 1.5 h de reposo, hipoclorito de sodio (NaClO) al 5% con media hora de reposo y fermentación a 48 y 72 h. La semilla extraída fue almacenada durante 90, 180 y 240 días a 4 °C. Se evaluó la germinación y vigor de la semilla. Se utilizó un diseño completamente al azar con arreglo factorial. Los resultados indicaron que la calidad fisiológica de la semilla, fue afectada por los efectos de deterioro que causa el almacenamiento y por los métodos de extracción de semilla; sin embargo, la semilla conserva su calidad fisiológica hasta los 240 días de almacenamiento, en los tratamientos a base de HCl al 0.3% y fermentación por 24 h con 93.3 y 91.2% de germinación después de 240 días de almacenamiento. Abstract in english It is very scarce available information related to post-harvest handling of papaya seed (Carica papaya L.) and their influence in seed physiologic quality, mainly in germination and vigor. The investigation work was carried out in seeds production laboratory of Center of Training and Development of [...] Seeds Technology, in greenhouse number one of Universidad Autónoma Agraria "Antonio Narro" (UAAAN) during 2008. Aim of work was to evaluate six treatments of seed extraction: hydrochloric acid was used (HCl) at 0.3 and 0.5% during one hour of rest, sulfuric acid (H2SO4) at 0.3% with 1.5 h of rest, sodium hypochlorite (NaClO) at 5% with 30 minutes of rest and fermentation at 48 and 72 h. Extracted seed was stored during 90, 180 and 240 days at 4 °C. It was evaluated seed germination and vigor. A totally random design was used with factorial arrangement. Results indicated that seed physiologic quality was affected by deterioration effects caused by storage and by methods of seed extraction; however, seed conserves its physiologic quality up to 240 days of storage, in treatments with help of HCl at 0.3% and fermentation for 24 h with 93.3 and 91.2 % germination after 240 days of storage.

  8. AKTIVITAS ANTIBAKTERI GETAH PEPAYA KERING TERHADAP Staphylococcus aureus PADA DANGKE [Antibacterial Activity of Dried Papaya Latex toward Staphylococcus aureus in Dangke

    Directory of Open Access Journals (Sweden)

    Rifah Hestyani Arum

    2014-06-01

    Full Text Available Dangke is a traditional milk curd product, made by coagulation of milk using fresh papaya latex. This product is usually kept at room temperature (27-30ºC until consumption. Dried papaya latex was used in this study to produce dangke, and its effect to S. aureus was determined by direct contact in TSB and dangke. Fresh papaya latex was dried using vacuum oven at 50-55ºC for 22 hours. Dried papaya latex at a concentration of 2.7x10-3 g/100 mL could reduce S. aureus approximately 1 log CFU/mL in TSB after 24 hours. Dried papaya latex and papain could maintain the S. aureus number in dangke within 24 hours storage at room temperature. The antibacterial activity of non-proteolytic compound of papaya latex, i.e ethanolic extract of papaya latex was determined by macrodilution method, resulted an the MIC90 of 8 mg/mL. The cell membrane leakage after exposure was detected by measuring the optical density of bacterial supernatant at 260 nm. The result showed that exposure to increasing antibacterial concentration resulted in increasing of optical density of S. aureus supernatant, indicating that the antibacterial caused the S. aureus membrane leakage. Fluorescence microscopy imaging showed that S. aureus exposure to antibacterial caused membrane leakage thus gave Propidium Iodide (PI chance to penetrate into the cell, as indicated by changing of fluorescence color from green to red.

  9. Heat treatment and gamma radiation on the control of Colletotrichum gloeosporioides (Penz.) Penz. et Sacc., causal agent of the papaya fruits (Carica papaya L.) anthracnose; Tratamento termico e radiacao gamma no controle de Colletotrichum gloeosporioides (PENZ.) PENTZ et SACC., agente causal da antracnose em frutos de mamoeiro (Carica papaya L.)

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Tanni Maria Werneck da

    1988-12-01

    For controlling papaya anthracnose on stored fruits the effect of heat treatment and gamma radiation on Colletotrichum gloeosporioides was studied in vitro and in vivo. Some physiological studies with four isolades of C. gloeosporioides from papaya fruits concerning the best conditions for mycelial growth and sporulation were also performed. The results showed that the best conditions for isolates development in BDA were: temperature between 25 and 27{sup 0} C for mycelium radial growth; between 27 and 30{sup 0} C under (12 hours alternating periods of fluorescent). (author). 131 refs., 9 figs., 33 tabs.

  10. Papaya seedlings growth using a low-cost, automatic watering controller

    Directory of Open Access Journals (Sweden)

    Gustavo Cardoso de Oliveira Dias

    2013-06-01

    Full Text Available The study assessed growth and physiological parameters of 'Sunrise Golden' and 'Tainung 01' papaya seedlings grown in 280mL plastic tubes and watered using a low-cost automatic irrigation system adjusted to operate at substrate water tension for starting irrigation (STI of 3.0, 6.0 or 9.0 kPa. The water depths applied by the dripping system and drainage were monitored during germination and seedling growth. Germination, emergence velocity index (EVI, leaf area, plant height, shoot and root dry weight, stomatal conductance, relative water content (RWC and relative chlorophyll content (RCC were evaluated. Soil nutrient levels were determined by electrical conductivity (EC. Water use efficiency (WUE corresponded to the ratio of plant dry mass to depth of water applied. STI settings did not affect papaya germination or EVI. System configuration to 3.0 and 6.0 kPa STI exhibited the highest drainage and lowest EC and RCC, indicating soil nutrient loss and plant nutrient deficiency. Drainage was greater in tubes planted with the 'Tainung 01' variety, which developed smaller root systems and lower stomatal conductance than 'Sunrise Golden' seedlings. The highest values for shoot dry weight and WEU were obtained at 6.0 kPa STI for 'Sunrise Golden' (0.62 g and 0.69 g L-1 and at 9.0 kPa in 'Tainung 01' (0.35 g and 0.82 g L-1. RWC at 9.0 kPa STI was lower than at 3.0 kPa in both varieties. The results indicate that the low-cost technology developed for irrigation automation is promising. Even so, new studies are needed to evaluate low-flow irrigation systems as well as the nutrient and water needs of different papaya varieties.

  11. Manejo de plantas daninhas na cultura do mamoeiro Weed control in papaya

    Directory of Open Access Journals (Sweden)

    C.P. Ronchi

    2008-01-01

    Full Text Available Apesar de o controle de plantas daninhas na cultura do mamoeiro representar um dos componentes de grande importância do custo de produção, no mundo e no Brasil, a disponibilidade de informações sobre esse tema é escassa, o que reflete a pouca importância que tem sido dada ao assunto nas últimas décadas. Neste texto são discutidos alguns avanços no manejo integrado de plantas daninhas que têm sido alcançados em vários países, particularmente no tocante à manutenção da vegetação natural ou à introdução de leguminosas na entrelinha de cultivo. Não obstante, várias espécies de plantas daninhas foram identificadas como hospedeiras de vetores de importantes viroses à cultura, com destaque para Commelina benghalensis e Solanum americanum. Isso se reveste de grande importância para o manejo (seletivo das plantas daninhas, sobretudo na produção integrada de mamão. Também são discutidos, em especial para a região norte do Estado do Espírito Santo, a seletividade de herbicidas, os períodos de controle e as principais características e aspectos práticos sobre o manejo de plantas daninhas na cultura do mamoeiro.Despite the fact that weed control entails high costs to crop production, the availability of national and international papers regarding papaya weed control is scarce, showing that little importance has been given to this subject in the last decades. Some integrated weed management advances attamed in several countries are discussed, particularly in the use of both natural groundcover or leguminous plants in the crop interrows. However, several weed species have been identified as host plants of major virus vectors to the crop, such as C. benghalensis and S. americanum. Such information is of major importance for selective weed control, particularly in the integrated system of papaya production. The present review also examined herbicide selectivity, weedy periods as well as the main characteristics and practical aspects of weed control in papaya, focusing on the production areas of the northern Espírito Santo State.

  12. Informativeness of minisatellite and microsatellite markers for genetic analysis in papaya.

    Science.gov (United States)

    Oliveira, G A F; Dantas, J L L; Oliveira, E J

    2015-10-01

    The objective of this study was to evaluate information on minisatellite and microsatellite markers in papaya (Carica papaya L.). Forty minisatellites and 91 microsatellites were used for genotyping 24 papaya accessions. Estimates of genetic diversity, genetic linkage and analyses of population structure were compared. A lower average number of alleles per locus was observed in minisatellites (3.10) compared with microsatellites (3.57), although the minisatellites showed rarer alleles (18.54 %) compared with microsatellite (13.85 %). Greater expected (He = 0.52) and observed (Ho = 0.16) heterozygosity was observed in the microsatellites compared with minisatellites (He = 0.42 and Ho = 0.11), possibly due to the high number of hermaphroditic accessions, resulting in high rates of self-fertilization. The polymorphic information content and Shannon-Wiener diversity were also higher for microsatellites (from 0.47 to 1.10, respectively) compared with minisatellite (0.38 and 0.85, respectively). The probability of paternity exclusion was high for both markers (>0.999), and the combined probability of identity was from 1.65(-13) to 4.33(-38) for mini- and micro-satellites, respectively, which indicates that both types of markers are ideal for genetic analysis. The Bayesian analysis indicated the formation of two groups (K = 2) for both markers, although the minisatellites indicated a substructure (K = 4). A greater number of accessions with a low probability of assignment to specific groups were observed for microsatellites. Collectively, the results indicated higher informativeness of microsatellites. However, the lower informative power of minisatellites may be offset by the use of larger number of loci. Furthermore, minisatellites are subject to less error in genotyping because there is greater power to detect genotyping systems when larger motifs are used. PMID:26280323

  13. Pseudomonas syringae pv. tabaci in papaya seedlings / Pseudomonas syringae pv. tabaci em plântulas de mamoeiro

    Scientific Electronic Library Online (English)

    Luís Otávio S., Beriam; Irene M.G., Almeida; Suzete A.L., Destéfano; Eunice, Grabert; Denise M., Balani; Mariana, Ferreira; Júlio, Rodrigues Neto.

    2006-03-01

    Full Text Available É relatada a ocorrência natural de Pseudomonas syringae pv. tabaci causando sintomas de lesões foliares em plântulas de mamoeiro. O patógeno foi identificado por meio de testes bioquímicos, fisiológicos, serológicos e moleculares, além de ensaios de patogenicidade em plantas de mamoeiro, feijoeiro e [...] fumo. Os padrões de restrição obtidos com as enzimas Afa I, Alu I, Dde I, Hae III, Hpa II, Hinf I, Sal 3A I e Taq I, utilizando-se a técnica de PCR-RLFP da região espaçadora 16S-23S do DNA ribossômico, foram idênticos àqueles apresentados para P. s. pv. tabaci. Primers correspondentes ao gene hrpL de P. syringae foram também testados e os resultados obtidos permitiram agrupar as linhagens isoladas de mamão com P. s. pv. tabaci. Linhagens bacterianas estão depositadas na coleção de culturas IBSBF, Instituto Biológico, Campinas, sob n. 1687 e 1822. Abstract in english The natural occurrence of Pseudomonas syringae pv. tabaci causing leaf spot symptoms in papaya seedlings is reported. The pathogen was identified through biochemical, physiological, serological, and molecular assays and artificial inoculations in papaya plants. It was also shown that the strains wer [...] e pathogenic to bean and tobacco plants. The restriction patterns obtained with Afa I, Alu I, Dde I, Hae III, Hpa II, Hinf I, Sau 3A I and Taq I of the PCR-RFLP of 16S-23S DNAr were identical to the P. s. pv. tabaci patterns. Primers corresponding to hrpL gene of P. syringae were also tested and the results grouped the papaya strains with P s. pv. tabaci. Bacterial strains were deposited at Coleção de Culturas IBSBF, Instituto Biológico, Campinas, Brazil, under access numbers 1687 and 1822.

  14. Pseudomonas syringae pv. tabaci in papaya seedlings Pseudomonas syringae pv. tabaci em plântulas de mamoeiro

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    Luís Otávio S. Beriam

    2006-03-01

    Full Text Available The natural occurrence of Pseudomonas syringae pv. tabaci causing leaf spot symptoms in papaya seedlings is reported. The pathogen was identified through biochemical, physiological, serological, and molecular assays and artificial inoculations in papaya plants. It was also shown that the strains were pathogenic to bean and tobacco plants. The restriction patterns obtained with Afa I, Alu I, Dde I, Hae III, Hpa II, Hinf I, Sau 3A I and Taq I of the PCR-RFLP of 16S-23S DNAr were identical to the P. s. pv. tabaci patterns. Primers corresponding to hrpL gene of P. syringae were also tested and the results grouped the papaya strains with P s. pv. tabaci. Bacterial strains were deposited at Coleção de Culturas IBSBF, Instituto Biológico, Campinas, Brazil, under access numbers 1687 and 1822.É relatada a ocorrência natural de Pseudomonas syringae pv. tabaci causando sintomas de lesões foliares em plântulas de mamoeiro. O patógeno foi identificado por meio de testes bioquímicos, fisiológicos, serológicos e moleculares, além de ensaios de patogenicidade em plantas de mamoeiro, feijoeiro e fumo. Os padrões de restrição obtidos com as enzimas Afa I, Alu I, Dde I, Hae III, Hpa II, Hinf I, Sal 3A I e Taq I, utilizando-se a técnica de PCR-RLFP da região espaçadora 16S-23S do DNA ribossômico, foram idênticos àqueles apresentados para P. s. pv. tabaci. Primers correspondentes ao gene hrpL de P. syringae foram também testados e os resultados obtidos permitiram agrupar as linhagens isoladas de mamão com P. s. pv. tabaci. Linhagens bacterianas estão depositadas na coleção de culturas IBSBF, Instituto Biológico, Campinas, sob n. 1687 e 1822.

  15. Fractionation and purification of the enzymes stored in the latex of Carica papaya.

    Science.gov (United States)

    Azarkan, Mohamed; El Moussaoui, Anouar; van Wuytswinkel, Delphine; Dehon, Géraldine; Looze, Yvan

    2003-06-25

    The latex of the tropical species Carica papaya is well known for being a rich source of the four cysteine endopeptidases papain, chymopapain, glycyl endopeptidase and caricain. Altogether, these enzymes are present in the laticifers at a concentration higher than 1 mM. The proteinases are synthesized as inactive precursors that convert into mature enzymes within 2 min after wounding the plant when the latex is abruptly expelled. Papaya latex also contains other enzymes as minor constituents. Several of these enzymes namely a class-II and a class-III chitinase, an inhibitor of serine proteinases and a glutaminyl cyclotransferase have already been purified up to apparent homogeneity and characterized. The presence of a beta-1,3-glucanase and of a cystatin is also suspected but they have not yet been isolated. Purification of these papaya enzymes calls on the use of ion-exchange supports (such as SP-Sepharose Fast Flow) and hydrophobic supports [such as Fractogel TSK Butyl 650(M), Fractogel EMD Propyl 650(S) or Thiophilic gels]. The use of covalent or affinity gels is recommended to provide preparations of cysteine endopeptidases with a high free thiol content (ideally 1 mol of essential free thiol function per mol of enzyme). The selective grafting of activated methoxypoly(ethylene glycol) chains (with M(r) of 5000) on the free thiol functions of the proteinases provides an interesting alternative to the use of covalent and affinity chromatographies especially in the case of enzymes such as chymopapain that contains, in its native state, two thiol functions. PMID:12767335

  16. ANTICANCER EFFECTS OF CARICA PAPAYA IN EXPERIMENTAL INDUCED MAMMARY TUMORS IN RATS

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    Gurudatta M, Deshmukh YA, Naikwadi A A

    2015-07-01

    Full Text Available Objective: To evaluate the anticancer effect of Carica papaya in DMBA induced mammary tumors in rats. Methods: Wistar rats were divided in to five groups (n=6, Group-I (Normal control administered vehicle olive oil, Group-II, Group-III ,Group-IV and V induced mammary tumors by administering single dose of DMBA (7,12 Dimethyl benz(Aanthracene orally 65 mg/kg. Group-III was administered aqueous leaf extract of Carica papaya (ALQECP in a dose of 200 mg/kg body wt for a period of 3 months, group-IV has given ALQECP 200 mg/kg body wt for a period of 21 days post 3 months of tumor induction, group-V rats were administered a small dose of Carica papaya extract intra tumor locally in the region of tumor. Results: Values of CA15-3 were increased in group-II rats (tumor control significantly, whereas in group-III (prevention group the levels of CA15-3 were found to be reduced substantially and the P value < 0.001. Similarly, CA-15-3 levels were reduced significantly in group-IV (treatment groupand P<0.005. The levels of LDH were seen to be increased in group-II, where as in group-III LDH levels were decreased and P<0.001.similarly group-IV LDH levels also reduced significantly but not to the level of group-III. Conclusion: Among the various markers for the detection of cancer antigen-15(CA15-3 and lactate dehydrogenase (LDH are important biochemical parameters that give a clear understanding of the progression and proliferation of cancer cells. In this study it was found that there is increase in the levels of markers such as CA15-3 and LDH and also the tumor volume in tumor control, these marker levels were decreased by the administration of aqueous leaf extract of Carica papaya in a dose of 200 mg/kg body wt. ALQECP not only prevented the progression of cancer growth but also has significant effect in reducing the both CA15-3 and LDH levels in treatment group.

  17. Dependência micorrízica de variedades comerciais de mamoeiro Mycorrhizal dependency of papaya commercial varieties

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    Aldo Vilar Trindade

    2001-12-01

    Full Text Available O genótipo da planta é fator preponderante na resposta à micorriza. Avaliou-se, neste trabalho, a resposta a fósforo e o grau de dependência micorrízica de variedades de mamoeiro (Carica papaya L.. O experimento foi realizado em casa de vegetação da Embrapa-Centro Nacional de Pesquisa de Mandioca e Fruticultura, com quatro variedades, testando-se seis doses de P: 0, 20, 40, 80, 140, 280 mg dm-3 e três tratamentos fúngicos (um controle não-micorrizado e a inoculação de Glomus clarum ou Gigaspora margarita. Foram estimados os seguintes parâmetros: dose de P para máxima eficiência micorrízica; valor T'; benefício da aplicação do P; benefício micorrízico; eficiência simbiótica, e dependência micorrízica. Plantas não-micorrizadas apresentaram resposta quadrática ao P aplicado e as inoculadas seguiram modelo raiz-quadrático, com grande incremento de matéria seca da parte aérea nas doses baixas; o benefício de P foi maior nas variedades Sunrise Solo e Improved Sunrise Solo - Line 72/12, enquanto as variedades Baixinho de Santa Amália e Tainung nº 1 apresentaram benefício micorrízico proporcionalmente maior, resultando em maior eficiência simbiótica. As variedades de mamoeiro apresentaram a seguinte classificação quanto à dependência micorrízica: Baixinho de Santa Amália = Tainung nº 1 > Sunrise Solo = Improved Sunrise Solo - Line 72/12. A maior ou menor dependência relacionou-se com a capacidade da variedade em produzir raízes.Plant genome is an important factor in plant response to arbuscular fungi. This work was designed to evaluate the degree of response to P application and mycorrhizal dependence of different varieties of papaya (Carica papaya L.. The experiment was performed under greenhouse conditions at Embrapa-Centro Nacional de Pesquisa de Mandioca e Fruticultura, Cruz das Almas, BA, Brazil, using seedlings of four varieties, six rates of P: 0, 20, 40, 80, 140 and 280 mg dm-3, and three fungi treatments (one control uninoculated and inoculation with Glomus clarum or Gigaspora margarita. The following parameters were estimated: rate of P for maximum mycorrhizal efficiency; T' value; P benefit, mycorrhizal benefit, symbiotic efficiency, and mycorrhizal dependence. Control plants had a quadratic response to applied P, while those inoculated had a root-quadratic response with a great increment in growth at lower rates; phosphorus benefit was higher in Sunrise Solo and Improved Sunrise Solo - Line 72/12 varieties, while the other two varieties showed a higher mycorrhizal benefit, resulting in a higher symbiotic efficiency. Papaya varieties showed the following classification of mycorrhizal dependency: Baixinho de Santa Amália = Tainung nº 1 > Sunrise Solo = Improved Sunrise Solo - Line 72/12. Mycorrhizal dependence related with the capability of the variety to produce roots.

  18. EFECTO DEL ETILENO SOBRE EL ACC Y ACC OXIDASA EN LA MADURACION DE PAPAYA 'MARADOL'

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    Javier De la Cruz

    2010-01-01

    Full Text Available Frutos de papaya 'Maradol' (Carica papaya L. fueron tratados con etileno en aire sintético (0, 100, 300 y 500 uL L-1 en contenedores de fibra de vidrio a diferentes tiempos de exposición (8, 16 y 24 h. Después del tratamiento, los frutos fueron transferidos para su maduración a temperatura ambiente (25 °C. Los análisis efectuados en cada tratamiento fueron: firmeza, azúcares reducto-res, sólidos solubles, acidez titulable, color de cáscara y pulpa (Hue, velocidad de producción de etileno (VPE, contenido de ácido 1-aminociclopropano-1-carboxílico (ACC y actividad de la enzima formadora de etileno (ACC oxidasa. Los resultados indicaron que el primer cambio notable fue el color en la cáscara (amarillo y en la pulpa (naranja, típicos en un fruto listo para el consumo. Los frutos expuestos a 500 uL L-1 de etileno por 16 y 24 h mostraron un pico climatérico un día después del tratamiento, mientras que los frutos expuestos por solamente 8 h mostraron su pico climatérico 2 d después de la exposición al etileno. Las papayas expuestas a 500 uL L-1 de etileno por 16 y 24 h produjeron bajos valores de Hue en menos tiempo que el testigo, y su contenido de ACC aumentó a 2.7 nmol g-1 h-1, la actividad de la ACC oxidasa a 3 nL g-1 h-1, y la velocidad de producción de etileno (VPE a 24 nL g-1 h-1. Estas condiciones permitieron que se alcanzaran valores de firmeza de 5.2 kgf cm-2 y 12 % de sólidos solubles a los 6 d a 25 °C. El contenido de azúcares reductores, valores de pH y la acidez no mostraron diferen-cias significativas (P > 0.05 entre tratamientos. Los frutos testigo alcanzaron valores similares después de 8 d de maduración directa. Los resultados sugieren que es posible acelerar la maduración de papaya 'Maradol' de 8 a 3 d por exposición a atmósferas con etileno.

  19. BIOPROSPECTING: A TOOL TO CONSERVE CHILEAN BRYOPHYTES / BIOPROSPECCIÓN: UNA HERRAMIENTA PARA LA CONSERVACIÓN DE BRIÓFITAS CHILENAS

    Scientific Electronic Library Online (English)

    Jorge, Cuvertino-Santoni; Gloria, Montenegro.

    Full Text Available Esta revisión presenta el estado actual del conocimiento de la diversidad de las briófitas chilenas (musgos, hepáticas y antocerotas), de los metabolitos secundarios de estas plantas y de la actividad biológica de extractos obtenidos a partir de especies de poblaciones chilenas. Nuestro objetivo es [...] determinar el potencial de estas plantas como fuente de moléculas útiles para las industrias farmacéutica y agroalimentaria, y promover su conservación. Sólo de un 3,7% de las briófitas chilenas (55 spp.) han sido analizados químicamente sus extractos (metanol, éter, etanol, diclorometano, acetona y hexano). Se ha evaluado la actividad biológica sólo de cuatro especies de poblaciones chileno-argentinas (Porella chilensis (Lehm. & Lindenb.) Trevi., Riccardia polyclada (Mitt. ex Thurn) Hässel, Balantiopsis cancellata (Nees) Stephani, y Sphagnum magellanicum Brid.). La mayor parte de los estudios se ha concentrado en hepáticas de amplia distribución en el Hemisferio Austral y en los bosques templados de Chile y Argentina. Se discuten brevemente aspectos relacionados a la bioprospección como herramienta para la conservación, y al cultivo in vitro de briófitas con fines productivos y de conservación. Abstract in english Here, we present the current state of knowledge on the diversity of Chilean bryophytes (mosses, liverworts, and hornworts), the secondary metabolites present in these plants, and the biological activity of extracts from populations of Chilean species. Our goal is to establish the potential of these [...] plants as a source of useful molecules for pharmaceutical and agricultural industries, thus promoting their conservation. Only 3,7% of Chilean bryophytes (55 spp.) have been analyzed using chemical characterization of their extracts (methanol, ether, ethanol, dichloromethane, acetone and hexane). Only four species from Chilean and Argentinian populations (Porella chilensis (Lehm. & Lindenb.) Trevi., Riccardiapolyclada (Mitt. ex Thurn) Hässel, Balantiopsis cancellata (Nees) Stephani, and Sphagnum magellanicum Brid.) have been evaluated for biological activity. The majority of these studies have concentrated on liverworts widely distributed in the Southern Hemisphere and in the temperate forests of the southern half of Chile and adjacent Argentina. We briefly discuss aspects related to the use of bioprospecting as a conservation tool as well as the maintenance of in vitro bryophyte cultures with the goal of conservation and production.

  20. Creencias sobre Papanicolaou y cáncer cérvicouterino en un grupo de mujeres chilenas

    Scientific Electronic Library Online (English)

    María Teresa, Urrutia S.

    Full Text Available Antecedentes: Son variadas las descripciones en relación a las creencias y como estas se relacionan con las conductas preventivas en cáncer cérvicouterino. Objetivo: Describir las creencias que tienen un grupo de mujeres chilenas pertenecientes al sistema público de atención acerca del Papanicolaou [...] y cáncer cérvicouterino. Método: Estudio analítico de corte transversal realizado en 333 mujeres chilenas. Se estudiaron las creencias sobre el Papanicolaou y el cáncer cérvicouterino con un instrumento desarrollado y validado en población chilena (CPC-28). Resultados: El 96% de las mujeres refiere tener un Papanicolaou en los últimos 3 años. El antecedente familiar es reportado por el 49,8% como causa de cáncer cérvicouterino. La barrera principal para adherir al Papanicolaou es la falta de conocimiento en cuanto a la edad requerida. La principal señal de acción que impulsa a que la mujer adhiera al tamizaje es la indicación entregada por el doctor. El beneficio mas importante es el cuidado de la salud. El 14,1% señala la presencia de relaciones sexuales como necesaria para adherir al tamizaje. El 17,4% de las mujeres se percibe fuera de riesgo de desarrollar un cáncer cérvicouterino. Las 6 dimensiones estudiadas se correlacionan entre si. Conclusión: Las creencias deben ser consideradas al momento de intervenir una población, empezando por valorarlas y comprenderlas para posteriormente poder modificarlas. Abstract in english Background: Many reasons has been described about relation between beliefs and cervical cancer behaviors. Objective: To describe the beliefs about cervical cancer and Pap test in a group of chilean women. Method: Cross sectional and analytic study was done with 333 chilean women. Beliefs about cervi [...] cal cancer and Pap test was studied with the CPC-28 questionnaire, developed and validated in Chilean population. Results: 96% have had a Pap test in the last 3 years. Family history about cervical cancer was reported by 49.8% as a cause of cervical cancer. The principal barrier to Pap test was lack of knowledge about the age. The principal cue to action was the doctor recommendation. The benefit was the care of health. The sexual intercourse was reported by 14.1% as necessary to take a Pap test. The risk to develop cervical cancer is reported by 17.4%. The six dimensions studied were correlated between them. Conclusion: The beliefs must be considered to education programs, firstly understanding and then modifying them.

  1. Effect of temperature on the development and survival of immature stages of the carambola fruit fly, Bactrocera carambolae, and the Asian papaya fruit fly, Bactrocera papayae, reared on guava diet.

    Science.gov (United States)

    Danjuma, Solomon; Thaochan, Narit; Permkam, Surakrai; Satasook, Chutamas

    2014-01-01

    Members of the Bactrocera dorsalis Hendel (Diptera: Tephritidae) complex constitute well-recognized destructive pests of fruits in peninsular Thailand. The development and survival of immature stages of the carambola fruit fly, Bactrocera carambolae Drew & Hancock, and the Asian papaya fruit fly, Bactrocera papayae Drew & Hancock, were compared at six constant temperatures of 15, 20, 25, 27, 30, and 35°C, 70 ± 5% relative humidity, and a photoperiod of 12:12 (L:D). The objectives were to determine the effect of temperature on the developmental stages for optimizing rearing and to understand the geographical pattern of occurrence of these fruit fly species. A strong and positive linear relationship was observed between temperature and developmental rate of immature stages of B. carambolae. Similarly, a strong and positive linear relationship was observed between temperature and developmental rate of B. papayae. A temperature summation model was used to estimate the lower threshold temperature and the thermal constant. Bactrocera papayae was significantly faster in development and higher in survival and appeared to be better adapted to low temperatures than B. carambolae, as it exhibited the lowest threshold temperatures at all immature stages. The observed differences in response to various temperatures revealed to some extent the impact of temperature on these species' distribution in peninsular Thailand and other parts of the world. PMID:25368070

  2. Revisiting the enzymes stored in the laticifers of Carica papaya in the context of their possible participation in the plant defence mechanism.

    Science.gov (United States)

    El Moussaoui, A; Nijs, M; Paul, C; Wintjens, R; Vincentelli, J; Azarkan, M; Looze, Y

    2001-04-01

    In the tropical species Carica papaya, the articulated and anastomosing laticifers form a dense network of vessels displayed in all aerial parts of the plant. Damaging the papaya tree inevitably severs its laticifers, eliciting an abrupt release of latex. Besides the well-known cysteine proteinases, papain, chymopapain, caricain and glycyl endopeptidase, papaya latex is also a rich source of other enzymes. Together, these enzymes could provide an important contribution to plant defence mechanisms by sanitising and sealing the wounded areas on the tree. PMID:11361091

  3. Yeast ratio is a critical factor for sequential fermentation of papaya wine by Williopsis saturnus and Saccharomyces cerevisiae.

    Science.gov (United States)

    Lee, Pin-Rou; Kho, Stephanie Hui Chern; Yu, Bin; Curran, Philip; Liu, Shao-Quan

    2013-07-01

    The growth kinetics and fermentation performance of Williopsis saturnus and Saccharomyces cerevisiae at ratios of 10:1, 1:1 and 1:10 (W.:S.) were studied in papaya juice with initial 7-day fermentation by W.saturnus, followed by S. cerevisiae. The growth kinetics of W.?saturnus were similar at all ratios, but its maximum cell count decreased as the proportion of S. cerevisiae was increased. Conversely, there was an early death of S. cerevisiae at the ratio of 10:1. Williopsis saturnus was the dominant yeast at 10:1 ratio that produced papaya wine with elevated concentrations of acetate esters. On the other hand, 1:1 and 1:10 ratios allowed the coexistence of both yeasts which enabled the flavour-enhancing potential of W.saturnus as well as the ethyl ester and alcohol-producing abilities of S. cerevisiae. In particular, 1:1 and 1:10 ratios resulted in production of more ethyl esters, alcohols and 2-phenylethyl acetate. However, the persistence of both yeasts at 1:1 and 1:10 ratios led to formation of high levels of acetic acid. The findings suggest that yeast ratio is a critical factor for sequential fermentation of papaya wine by W.saturnus and S. cerevisiae as a strategy to modulate papaya wine flavour. PMID:23171032

  4. Effects of chitosan-based coatings containing peppermint essential oil on the quality of post-harvest papaya fruit

    Science.gov (United States)

    Edible coatings comprised of antimicrobial polymers based on chitosan are promising technologies to preserve post-harvest fruit quality. In this study, we investigated the potential utility of a coating made from chitosan modified by N-acylation with fatty acid to preserve post-harvest papaya qualit...

  5. An Efficient Seed Germination and Seedling Establishment Protocol for Hybrid Carica papaya Linn. With Application of Plant Growth Regulator

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    Ravishankar Chauhan

    2014-01-01

    Full Text Available In vitro germination of hybrid seeds of papaya (Carica papaya Linn. was attempted in the present investigation. Hybrid papaya seeds were subjected to three kinds of treatments. First on semisolid medium Murashige and Skoog (1962, secondly on only inorganic salt solution of the same medium but without organic nutrients and with various combinations of plant growth regulators on coco-peat as support material and in the third set of experiment, the seeds without seed-coat with 6-benzyl adenine having coco-peat as support material. Results showed that the germination rate of the papaya seeds was inhibited by the seed coat. Therefore, the best 90% in vitro germination was observed when the seeds without seed-coat were subjected to 0.5 mg L-1 6-benzyl adenine in coco-peat containing vessels. The seedlings were subjected to in vitro hardening. After three months of transfer to greenhouse and subsequently in nursery, the young seedlings showed 90% survival.

  6. VARYING GENETIC DIVERSITY OF PAPAYA RINGSPOT VIRUS ISOLATES FROM TWO TIME-SEPARATED OUTBREAKS IN JAMAICA AND VENEZUELA

    Science.gov (United States)

    The coat protein (CP) genes of eleven Jamaican Papaya ringspot virus type-p (PRSV) isolates that were collected in 1999 were cloned and sequenced. The nucleotide and amino acid sequences of these isolates were compared to each other, with a sequence of another Jamaican isolate collected after the f...

  7. Análisis de la política de pago de dividendos en empresas chilenas

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    MAURICIO GUTIÉRREZ URZÚA

    2012-01-01

    Full Text Available Este artículo analiza la tasa de pago de dividendos de las empresas chilenas, considerando aspectos como el pago de dividendos obligatorios, la fuerte concentración de la propiedad, las oportunidades de crecimiento y la presencia de inversionistas institucionales. La investigación se realizó para el período 2001 a 2007, a través de regresiones lineales múltiples, con selección de variables mediante el criterio de stepwise y estimación de parámetros a través de máxima verosimilitud, en donde se encontró evidencia de la utilización de la tasa de pago de dividendos para entregar información sobre los proyectos futuros de la empresa y el tratamiento justo de los accionistas. También se destaca el control ejercido por los inversionistas institucionales.

  8. Determinantes de la Competitividad de las Exportaciones de Uva de Mesa Chilena, 1984-2004

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    Arcadio Cerda U.

    2011-01-01

    Full Text Available La industria frutícola chilena ha logrado situarse como una de las más prosperas en la canasta exportadora del país y ha tenido una signifi cativa importancia en el mercado mundial, logrando posicionarse como el segundo exportador a nivel mundial. Dado lo anterior, estudiar la competitividad de dicha industria es importante para el desarrollo exportador del sector agroindustrial chileno y para la economía en general. Por ello, el objetivo de este trabajo fue medir la competitividad de la uva de mesa a través del índice de Vollrath (1991 y estudiar sus determinantes. El análisis del indicador demostró que la competitividad fue, en general, creciente hasta el año 2002 y que las principales determinantes de la competitividad son el tipo de cambio real y el producto interno bruto real.

  9. La historia de la Iglesia en la historiografía chilena 1965-2015

    Directory of Open Access Journals (Sweden)

    Douglas Barry W.

    2015-01-01

    Full Text Available La historia de la Iglesia en Chile ha sido tra - tada desde múltiples perspectivas, tanto por historiado - res eclesiásticos como civiles. Igualmente variadas son las temáticas abordadas por dichos autores, que refle - jan la transversalidad de la incidencia e influencia de la Iglesia en distintos ámbitos de la vida nacional tanto en el periodo hispano, en el proceso de Independencia y en su vida republicana. En el presente artículo se exponen las principales aportaciones que en los últimos cincuen - ta años ha brindado la historiografía chilena a la historia de la Iglesia, de acuerdo a una selección de temas que vienen a completar los presentados en la primera parte de este trabajo, tales como la recopilación y transcrip - ción de archivos eclesiásticos, la historia de las iglesias particulares o locales, los aspectos jurídico eclesiásticos y las manifestaciones culturales y artísticas.

  10. El delito de estafa en la jurisprudencia chilena The swindle crime in the jurisprudence of Chile

    Directory of Open Access Journals (Sweden)

    Gustavo Balmaceda Hoyos

    2011-07-01

    Full Text Available En el presente trabajo se expone la interpretación que estimamos han asumido los tribunales chilenos sobre el delito de estafa, valorando si se trata de un juicio coherente con la técnica chilena de tipificación, idiosincrasia, y que responda de mejor manera a las necesidades político-criminales del presente, durante el período de tiempo comprendido entre 1841 y 2010.The present work exposes the interpretation that we considered has assumed the Chilean courts about the swindle crime, valuing if it is a coherent judgment with the Chilean standardization's technique of felonies, idiosyncrasy, and if it responds the better way to our present's criminal policy, during the period of time between 1841 and 2010.

  11. Evolución de la industria chilena de fondos mutuos. Análisis de su performance (2000-2005

    Directory of Open Access Journals (Sweden)

    Benito Umaña Hermosilla

    2013-05-01

    Full Text Available En el presente estudio, los autores desarrollan un análisis riguroso de la evolución de la Industria Chilena de Fondos Mutuos. Se aplican para ello las medidas tradicionales de performance,  correspondientes a los autores Sharpe, Treynor y Jensen, poniendo para ello de manifiesto, que su funcionamiento correcto depende de una serie de circunstancias, que no siempre se cumplen en los mercados financieros. En estos casos se aplican  medidas alternativas de medición de la performance, las cuales eliminan las incoherencias detectadas. Estas medidas fueron desarrolladas por los profesores Ferruz y Sarto (1997. El presente estudio comprende el periodo 2000- 2005. Se analizan rentabilidades mensuales correspondientes a 196 fondos mutuos de renta variable. Los índices alternativos no hicieron más que ratificar el resultado de los índices originales. Se concluye además que las gestoras con mayor patrimonio administrado no poseen los mejores índices de performance, similar  situación ocurre con aquellas gestoras pertenecientes a la industria bancaria.

  12. La ambivalente representación del roto en la poesía popular chilena

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    Pamela Tala Ruiz

    2011-11-01

    Full Text Available Este artículo examina la representación ambivalente de la figura del roto en la poesía popular chilena impresa en hojas sueltas entre fines del siglo XIX y principios del XX. Se estudia la manera en que se construye desde la cultura popular la celebración del roto en tanto representante de la nación (el soldado anónimo que lucha contra la amenaza extranjera, por un lado, y un rechazo en su mismo grupo social (en tanto se le asocian significados como vagancia, robo, inestabilidad, por otro. Se comprueba que la representación del roto como sujeto de la enunciación de estos poemas es un ejemplo más de su compleja identidad.

  13. Integración metodológica para el estudio del texto de las sentencias penales chilenas

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    Claudio Antonio Agüero San Juan

    2010-01-01

    Full Text Available Proponemos una metodología para describir la estructura y el funcionamiento del texto "sentencia penal chilena" y evaluar la calidad de su argumentación. La importancia de estos procesos radica en que la sentencia es un caso paradigmático del modo en que las sociedades construyen lo bueno y lo malo. En virtud de la especificidad del género y del registro lingüístico del texto en estudio, integramos elementos de pragma-dialéctica, de lingüística sistémico funcional y de Modified Wigmorean Analysis para suplir las desventajas individuales de cada metodología y optimizar sus ventajas. Se revisa someramente el aparato crítico que sustenta cada una de las estrategias de análisis, luego, a través del estudio de un fragmento de una sentencia real, demostramos la factibilidad de la integración y concluimos revisando la posibilidad de usar esta propuesta en comparaciones intertextuales e interdiscursivas.

  14. Sorologia e sobrevivência do vírus do amarelo letal do mamoeiro Serology and surviving characteristics of Papaya lethal yellowing virus

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    Ana Lúcia Lopes do Nascimento

    2010-09-01

    Full Text Available O vírus do amarelo letal do mamoeiro (Papaya lethal yellowing virus, PLYV é responsável por uma das principais doenças do mamoeiro (Carica papaya no Nordeste brasileiro. O PLYV pode ser transmitido através do solo, água, instrumento de corte e mãos contaminadas. A presente pesquisa teve como objetivo estudar as características biológicas, sorológicas e físicas de um isolado do vírus e avaliar sua sobrevivência em tecido seco infetado. O PLYV foi detectado por "Enzyme linked immunosorbent assay" (ELISA indireto e isolado em mudas de mamoeiro, através da inoculação mecânica. A sobrevivência do PLYV em folhas e raízes secas de mamoeiro infetado foi avaliada por sorologia e inoculação em plantas sadias. A presença do vírus foi detectada em folhas e raízes de mamoeiro secas, indicando que o vírus pode permanecer ativo em restos de cultura por até 120 dias. A purificação do PLYV permitiu a obtenção de 309.5 mg de vírus/kg de folha e o anti-soro obtido mostrou-se altamente específico, com títulos de 1:128 em dupla difusão em Agar e 1:1.024.000 em Elisa indireto. Estudos das propriedades físicas do PLYV em mamoeiro revelaram um ponto de inativação térmica (PIT em torno de 80 ºC, um ponto máximo de diluição (PMD de ac. 10-6 e uma longevidade in vitro (LIV acima de 50 dias.The Papaya lethal yellowing virus (PLYV is responsible for one of the most important disease of papaya (Carica papaya in the Northeast of Brazil. The PLYV can be transmitted through the soil, irrigation water, agriculture tools and contaminated hands. The present research had the objective to characterize biologically, serologically and physically a PLYV isolate and evaluates how long it survives in infected dried tissues. The PLYV was identified by indirect enzyme linked immunoabsorbent assay (ELISA, isolated in young papaya plants by mechanical inoculations and maintained at green house conditions for virus purification. Approximately 309.5 mg of virus was purified per kg of infected papaya leaves and a polyclonal antiserum was obtained from an immunized rabbit. The antiserum obtained was shown to be highly specific to PLYV with a titer of 1:128 in double immune-diffusion and a titer of 1:1.024.000 in indirect ELISA. The virus was detected in dried roots and leaves maintained at room temperature up to 120 days, confirming its high stability within nonliving plant tissues, which could explain its dissemination by contaminated hands, tools, water and soil. The physical properties determined for the virus revealed a thermal inactivation point of 80 ºC, longevity in vitro over 50 days and dilution end point ac. of 10-6.

  15. Pressão hidrostática nos atributos sensoriais do néctar de mamão / Hydrostatic high pressure in sensory attributes of papaya nectar

    Scientific Electronic Library Online (English)

    Fernanda Branco, Shinagawa; Rosires, Deliza; Amauri, Rosenthal; Maria Alice, Zarur.

    2013-10-01

    Full Text Available A indústria de alimentos utiliza a análise sensorial como ferramenta essencial no desenvolvimento, otimização, controle de qualidade e análise do potencial mercadológico de novos produtos. A alta pressão hidrostática (APH) é uma tecnologia inovadora que permite manter a qualidade dos alimentos em te [...] rmos nutricionais e sensoriais, bem como o frescor natural e aumentar a sua vida útil. A APH não afeta ligações covalentes, portanto são mínimos os efeitos nos constituintes químicos responsáveis pela cor, sabor e conteúdo nutricional. Este estudo teve como objetivo investigar o efeito da APH aplicada à polpa de mamão nos atributos sensoriais do néctar. Para tal, polpa controle (sem tratamento), pasteurizada (92°C 40s-1) e pressurizada (300MPa 5min-1 25°C-1) e quatro marcas comerciais de polpa de mamão congeladas foram utilizadas para preparar os sete respectivos néctares. Estudos iniciais foram realizados para determinação da quantidade de água e açúcar “ideal” a serem adicionadas à polpa para o preparo do néctar, de acordo com a resposta dos consumidores. As sete amostras foram analisadas por 12 provadores selecionados e treinados, utilizando a Análise Descritiva Quantitativa (ADQ). Os resultados mostraram que os néctares das amostras controle e pressurizada foram similares quanto aos atributos sensoriais, tendo sido caracterizadas pela presença de grumos, sabor característico de mamão, consistência e aroma característico de mamão. Abstract in english The food industry uses the sensory evaluation as an essential tool in the development, optimization, quality control and the analysis of new product potential market. High hydrostatic pressure (HHP) is an innovative technology that allows retaining the nutritional and sensory food quality, as well a [...] s the natural freshness, and extends product shelf life. The HHP does not affect covalent bonds; therefore, it has little effect on chemical constituents responsible for the color, flavor and nutritional content. This study aimed at evaluating the effect of HHP applied to the papaya pulp on sensory attributes of papaya nectar. Control (without treatment), pasteurized (92°C 40s-1) and pressurized papaya purée (300MPa 5min-1 25°C-1) and four commercial papaya frozen purées available in the market were used to prepare the seven respective papaya nectars. Preliminary studies were carried out to determine the amount of water and sugar to be added to the purée, according to the consumer preference. The seven samples were evaluated by 12 selected and trained panelists using the Quantitative Descriptive Analysis (QDA). The results showed that the nectars of control and pressurized samples were similar regarding sensory attributes having been characterized by the presence of lumps, characteristic flavor of papaya, consistency and characteristic aroma of papaya.

  16. Beverages of lemon juice and exotic noni and papaya with potential for anticholinergic effects.

    Science.gov (United States)

    Gironés-Vilaplana, Amadeo; Valentão, Patrícia; Andrade, Paula B; Ferreres, Federico; Moreno, Diego A; García-Viguera, Cristina

    2015-03-01

    Lemon (Citrus limon (L.) Burm. f.) juice beverages enriched either with noni (Morinda citrifolia L.) (LN) or papaya (Carica papaya L.) (LP), were characterized by HPLC-DAD-ESI/MS(n), the antioxidant capacity was evaluated by (DPPH·), superoxide (O2(·-)), hydroxyl radicals (·OH) and hypochlorous acid (HOCl) assays, and their potential as acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitors was also assessed. The fruits are rich in a wide range of bioactive phenolics. Regarding DPPH·, ·OH and HOCl assays, the LP displayed strong activity, and LN was the most active against O2(·-). Concerning cholinesterases, LP was the most active, mainly due to lemon juice contribution. The effect on the cholinesterases was not as strong as in previous reports on purified extracts, but the bioactive-rich beverages offer the possibility of dietary coadjutants for daily consumption of health-promoting substances by adults with aging-related cognitive or physical disorders. PMID:25306312

  17. EFFECT OF PAPAYA LEAF JUICE ON PLATELET AND WBC COUNT IN DENGUE FEVER: A CASE REPORT

    Directory of Open Access Journals (Sweden)

    Lakshmiprasad L Jadhav

    2013-08-01

    Full Text Available Dengue fever caused by dengue viruses (dengue 1–4 having Aedes aegypti mosquito as their principal vector, causes symptoms such as sudden onset of fever, headache, retro-orbital pain  and back pain along with severe myalgia due to which dengue fever is also known as “break-bone fever.” Laboratory findings include leukopenia, thrombocytopenia and in many cases, serum aminotransferase elevations. dengue hemorrhagic fever (DHF or dengue shock syndrome (DSS may occur as a complication of dengue fever.A pilot study and a randomized controlled trial showed that administration of papaya leaf juice was beneficial in dengue patients in elevating the total white cell counts and platelet counts. Based on this report, a dengue patient with thrombocytopenia and leukopenia was treated in a tertiary Ayurveda hospital. The patient was administered papaya leaf juice in the dose of 25 ml twice daily along with conventional line of management for a period of eight days. There was remarkable improvement in the subjective symptoms and the white blood cell count and platelet count were restored to normalcy.

  18. Control of papaya fruits anthracnose by essential oil of Ricinus communis

    Scientific Electronic Library Online (English)

    César Luis, Siqueira Júnior; Maria das Graças Machado, Freire; Antônio Sérgio Nascimento, Moreira; Maria Ligia Rodrigues, Macedo.

    2012-02-01

    Full Text Available The aim of this work was to investigate the potential of castor oil for the control of papaya diseases caused by the fungus Colletotrichum gloeosporioides and the bacterium Pseudomonas caricapapayae. The treatment with 1% castor oil did not significantly affect the fungal growth. The effectiveness o [...] f castor oil for the control of anthracnose was shown when 5% and 10% (v/v) were used in the assays resulting in reduced mycelial growth. Fungal sporulation was strongly inhibited at 10% (v/v) concentration of essential oil. The studies with the fresh fruits treated with 5% (v/v) castor oil in aqueous emulsions resulted in effective reduction of pathogen spread in these fruits. No lesion was found in the fruits treated with oil, when compared to the control fruits. Castor oil showed no effect against the P. caricapapayae when tested in vitro. These results suggested the potential use of the castor bean essential oil and its fatty acids constituents for the control of anthracnose in papaya fruits.

  19. Prediction of genetic gain from selection indices for disease resistance in papaya hybrids

    Directory of Open Access Journals (Sweden)

    Marcelo Vivas

    2012-12-01

    Full Text Available In order to select superior hybrids for the concentration of favorable alleles for resistance to papaya black spot, powdery mildew and phoma spot, 67 hybrids were evaluated in two seasons, in 2007, in a randomized block design with two replications. Genetic gains were estimated from the selection indices of Smith & Hazel, Pesek & Baker, Williams, Mulamba & Mock, with selection intensity of 22.39%, corresponding to 15 hybrids. The index of Mulamba & Mock showed gains more suitable for the five traits assessed when it was used the criterion of economic weight tentatively assigned. Together, severity of black spot on leaves and on fruits, characteristics considered most relevant to the selection of resistant materials, expressed percentage gain of -44.15%. In addition, there were gains for other characteristics, with negative predicted selective percentage gain. The results showed that the index of Mulamba & Mock is the most efficient procedure for simultaneous selection of papaya hybrid resistant to black spot, powdery mildew and phoma spot.

  20. Allomonal and hepatotoxic effects following methyl eugenol consumption in Bactrocera papayae male against Gekko monarchus.

    Science.gov (United States)

    Wee, S L; Tan, K H

    2001-05-01

    Methyl eugenol (ME), is converted into two major phenylpropanoids, 2-allyl-4,5-dimethoxyphenol and trans-coniferyl alcohol, following consumption by the male fruit fly Bactrocera papayae. Chemical analysis of wild male B. papayae rectal glands, where the compounds are sequestered, revealed the presence of ME metabolites in varying quantities. These phenylpropanoids are shown to be involved in the fruit fly defense both in no-choice and choice feeding tests against the Malayan spiny gecko, Gekko monarchus. After being acclimatized to feeding on fruit flies, geckos consumed significantly fewer ME-fed male flies than controls that consumed all the ME-deprived male flies offered throughout a two-week period. Diagnosis of dissected livers from geckos that consumed ME-fed male flies revealed various abnormalities. These included discoloration and hardening of liver tissue, whitening of the gallbladder, or presence of tumor-like growths in all geckos that consumed ME-fed male flies. Control geckos fed on ME-deprived male flies had healthy livers. When given an alternative prey, geckos preferred to eat untreated house flies, Musca domestica to avoid preying on ME-fed fruit flies. PMID:11471947

  1. Compositional and functional dynamics of dried papaya as affected by storage time and packaging material.

    Science.gov (United States)

    Udomkun, Patchimaporn; Nagle, Marcus; Argyropoulos, Dimitrios; Mahayothee, Busarakorn; Latif, Sajid; Müller, Joachim

    2016-04-01

    Papaya has been identified as a valuable source of nutrients and antioxidants, which are beneficial for human health. To preserve the nutritional properties after drying, appropriate storage specifications should be considered. This study aimed to investigate the quality and stability of air-dried papaya in terms of quality dynamics and behavior of bio-active compounds during storage for up to 9 months in two packaging materials: aluminum laminated polyethylene and polyamide/polyethylene. Samples with moisture content (MC) of 0.1328 g g(-1) and water activity (aw) of 0.5 were stored at 30 °C and relative humidity (RH) of 40-50%. The MC, aw, degree of browning (DB) and 5-hydroxymethylfurfural (HMF) content were found to notably increase as storage progressed. On the contrary, there was a significant decrease in antioxidant capacity (DPPH, FRAP and ABTS), total phenolic (TP) and ascorbic acid (AA) contents. Packaging in aluminum laminated polyethylene under ambient conditions was found to better preserve bio-active compounds and retard increases in MC, aw and DB, when compared to polyamide/polyethylene. PMID:26593545

  2. Control of papaya fruits anthracnose by essential oil of Ricinus communis

    Directory of Open Access Journals (Sweden)

    César Luis Siqueira Júnior

    2012-02-01

    Full Text Available The aim of this work was to investigate the potential of castor oil for the control of papaya diseases caused by the fungus Colletotrichum gloeosporioides and the bacterium Pseudomonas caricapapayae. The treatment with 1% castor oil did not significantly affect the fungal growth. The effectiveness of castor oil for the control of anthracnose was shown when 5% and 10% (v/v were used in the assays resulting in reduced mycelial growth. Fungal sporulation was strongly inhibited at 10% (v/v concentration of essential oil. The studies with the fresh fruits treated with 5% (v/v castor oil in aqueous emulsions resulted in effective reduction of pathogen spread in these fruits. No lesion was found in the fruits treated with oil, when compared to the control fruits. Castor oil showed no effect against the P. caricapapayae when tested in vitro. These results suggested the potential use of the castor bean essential oil and its fatty acids constituents for the control of anthracnose in papaya fruits.

  3. Effect of Prior Heat Stress on the Early Growth of Carica papaya

    Directory of Open Access Journals (Sweden)

    Gideon Olarewaju OKUNLOLA

    2013-12-01

    Full Text Available The experiment was carried out to determine the effects of heat stress on some growth parameters like shoot height, leaf area, fresh weight, dry weight as well as the accumulation of chlorophylls in Carica papaya. Seedlings of C. papaya were exposed to prior heat stress at 40 °C. A group of plants was placed in a Gallenkamp oven for four hours; another group of plants was placed in the oven for eight hours while the third group of plants was placed in a dark cupboard for the period of eight hours. Sampling was carried out at weekly intervals starting from seven days after treatment. Plants were randomly picked from each of the three treatments. Three replicates were used for each parameter. The results obtained from the study showed that there was an increment in the shoot height, leaf area, fresh weight and dry weight from the beginning to the end of the experimental period. However, the accumulation of chlorophylls did not follow a particular pattern. The analysis of variance carried out on the data obtained showed that heat stress had a significant effect on the petiole length, shoot height, leaf length, leaf width, leaf area, fresh weight and dry weight. Heat stress, however, did not produce a significant effect on the accumulation of chlorophylls a and b and total chlorophyll.

  4. Water depletion on substrate and Osmocote? fertilizer dose in the papaya seedlings formation

    Directory of Open Access Journals (Sweden)

    Júlio César Ferreira de Melo Júnior

    2014-01-01

    Full Text Available The successful of fruit production depends on the high quality seedlings use, given that the initial growth of papaya seedlings is related to the precocity and quality of the fruit production. In this context, the aim of this study was to evaluate the different water depletion levels in substrate defined as a mass fraction of container capacity (MFCC as well as Osmocote® fertilizer dose (14-14-14 in the formation of papaya seedlings in containers. The experimental design was in randomized block in split plot with four replications, defined as plot the water depletion level of substrate (5, 15, 25 and 35% of MCC and subplot as the fertilizer dose (0, 3, 6, 9, 12 and 15 kg m-3. The shoot height, stem diameter, number of leaves, dry fitomassas of shoot, root and total, the dry mass relation between shoots and roots, and the Dickson quality index were determined. All caracteristics were subjected to variance analysis, and Scott-Knott test and regression analysis for water level depletion and fertilizer dose. The interaction between treatments that provided the best responses of seedlings was that subjected to depletion of 15% of the MCC and the fertilizer dose of 8.5 kg m-3.

  5. Cancer chemopreventive effects of the flavonoid-rich fraction isolated from papaya seeds.

    Science.gov (United States)

    Pathak, Neelam; Khan, Saba; Bhargava, Arpit; Raghuram, Gorantla V; Jain, Deepika; Panwar, Hariom; Samarth, Ravindra M; Jain, Subodh K; Maudar, Kewal K; Mishra, Dinesh K; Mishra, Pradyumna K

    2014-01-01

    Intervention to decelerate, arrest, or reverse the process of carcinogenesis by the use of either natural or synthetic agents individually or in combination has emerged as a promising and pragmatic medical approach to reduce cancer risk. In the present study, we examined the cancer chemopreventive potential of a flavonoid-rich fraction isolated from the seeds of Carica papaya, a plant traditionally referred to as papaw. The flavonoid-enriched benzene fraction of the aqueous extract exerted its anticancer properties in vitro through cytoprotection, antioxidative and antiinflammatory mechanisms and genoprotection in response to isocyanate-induced carcinogenicity. Medium-term anticarcinogenicity and 2-stage skin papillomagenesis studies conducted in benzopyrene-induced lung carcinogenesis and 7,12-dimethyl benz(a)anthracene-mediated skin papillomagenesis mouse models further validated our in vitro observations. This is the first demonstration of chemopreventive activities of papaya seed products, however, further studies to understand the subtle targets of intracellular signaling pathways, pharmacological profile and toxicological safety of this bioactive fraction are essential to pave the way for successful clinical translation. Our study supports the inverse association between dietary flavonoid intake and cancer risk. PMID:24820939

  6. Kinetic constants for the hydrolysis of aggrecan by the papaya proteinases and their relevance for chemonucleolysis.

    Science.gov (United States)

    Dekeyser, P M; Buttle, D J; Devreese, B; Van Beeumen, J; Demeester, J; Lauwers, A

    1995-07-10

    The four known proteinases from papaya latex, namely papain (EC 3.4.22.2), chymopapain (EC 3.4.22.6), caricain (EC 3.4.22.30), and glycyl endopeptidase (EC 3.4.22.25), were purified to homogeneity and fully characterized by single radial immunodiffusion and active-site titration. A modified HPLC gel permeation assay was used to determine the kinetic constants for aggrecan hydrolysis by the papaya proteinases. The disappearance of intact aggrecan monomer was first-order, indicating that for the four enzymes studied the Km was much larger than 0.5 microM and that kcat/Km = 1.2 +/- 0.1 x 10(6) M-1 s-1 for chymopapain, 1.20 +/- 0.08 x 10(6) M-1 s-1 for caricain, 0.90 +/- 0.02 x 10(6) M-1 s-1 for papain, and 0.120 +/- 0.005 x 10(6) M-1 s-1 for glycyl endopeptidase. Chymodiactin, the chymopapain preparation used for chemonucleolysis, consists of a mixture of chymopapain (70%), caricain (20%), and glycyl endopeptidase (4%). The rate constant for the aggrecan hydrolysis by such a mixture was not significantly different from the rate constant for pure chymopapain. As a result of these observations, we predict that pure chymopapain could replace partially purified chymopapain preparations for chemonucleolysis. PMID:7625846

  7. Heterosis in papaya: inter and intragroup analysis / Heterose no mamão: analise inter e intra-grupo

    Scientific Electronic Library Online (English)

    Deisy Lúcia, Cardoso; Lucas Nunes da, Luz; Celia Maria Peixoto de, Macêdo; Leandro Gonçalves Simões, Azeredo; Messias Gonzaga, Pereira.

    2014-09-01

    Full Text Available O mamão (Carica papaya L.) é uma cultura típica de áreas tropicais e o Brasil é um dos principais produtores mundiais. Nas últimas décadas, a cultura de mamão se expandiu para diferentes regiões do país, mas o número de cultivares disponíveis ainda é limitado. No presente trabalho, um cruzamento dia [...] lélico completo foi realizado com oito acessos de mamão do banco de germoplasma da UENF/Caliman. Quatro genótipos pertencem ao grupo heterótico Formosa e quatro, ao grupo Solo. Este trabalho teve como objetivo avaliar a ocorrência e viabilidade de explorar a heterose em híbridos heteróticos intra-grupo. Cinquenta e seis progénies híbridas foram geradas e avaliadas. Entre os híbridos intra-grupo Formosa, duas combinações híbridas (MR x J4 e MR x SK) apresentaram heterose para todas as características, bem como uma boa produção média total de frutos. Entre os híbridos intra-grupo Solo, três combinações híbridas (WM x GG, WM x SS e WM x SM) destacam-se pela produção de frutas e alto teor de sólidos solúveis. Em Formosa x híbridos de solo, todas as combinações híbridas com o progenitor JS (JS x WM, JS x GG, JS x SS e JS x SM) apresentaram frutos de alta qualidade e boa média para produção de frutos. O perfil de heterose dos híbridos testados permitiu a identificação de híbridos promissores dentro dos grupos heteróticos Formosa e Solo. A análise das variáveis canónicas também possibilitou a visualização de grupos distintos de híbridos, de acordo com a proveniência dos progenitores. Abstract in english Papaya (Carica papaya L.) is a typical crop of tropical areas, and Brazil is one of the leading world producers. In recent decades, papaya culture has expanded to different regions of the country, but the number of cultivars available is still limited. In the present study, a complete diallel cross [...] was carried out using eight accessions of papaya from the UENF/Caliman germplasm bank. Four genotypes belong to the Formosa heterotic group and four, to the Solo group. This study aimed to evaluate the occurrence and viability of exploring heterosis in heterotic intragroup hybrids. Fifty-six hybrid progenies were generated and evaluated. Among the Formosa intragroup hybrids, two hybrid combinations (MR x J4 and MR x SK) showed heterosis for all traits, as well as good average total fruit production. Among the Solo intragroup hybrids, three hybrid combinations (WM x GG, WM x SS and WM x SM) stand out for fruit production and high content of soluble solids. In Formosa x Solo hybrids, all hybrid combinations with the parent JS (JS x WM, JS x GG, JS x SS and JS x SM) showed high fruit quality and good average for fruit production. The heterotic profile of the hybrids tested allowed the identification of promising hybrids within Formosa and Solo heterotic groups. The analysis of the canonical variables also allowed the visualization of distinct groups of hybrids, depending on the provenance of the parents.

  8. INVENTARIO DE GASES CON EFECTO INVERNADERO EMITIDOS POR LA ACTIVIDAD AGROPECUARIA CHILENA Inventory of greenhouse gas emissions by Chilean agriculture

    OpenAIRE

    Rafael Novoa S.A.; Sergio González M.; Rosemary Novoa J.; Rosa Rojas

    2000-01-01

    Se realizó una estimación de los gases con efecto invernadero emitidos por la agricultura chilena. Los resultados indican que el año 1994, la agricultura chilena emitió 321,92 Gg de metano, 21,80 Gg de N2O; 2,96 Gg de NOx y 51,97 Gg de CO. Además, se estimó que las emisiones de COVNM llegan a 2,59 Gg año-1. Estas cifras expresadas como porcentaje de las emisiones del sector no energía chileno llegan a un 74,3 % para el metano, un 5,1 % para el CO; un 93,8 % para el N2O; un 9,8 % para los NOx ...

  9. Validación Chilena del Cuestionario de Evaluación del Apego Romántico Experiences in Close Relationships (ECR: Análisis de la Validez de Criterio

    Directory of Open Access Journals (Sweden)

    Rosario Spencer

    2013-01-01

    Full Text Available El objetivo de esta investigación fue analizar la validez de criterio del cuestionario de apego romántico Ex - periences in Close Relationships (ECR, Brennan, Clark y Shaver, 1998 para la población chilena de adultos. Este cuestionario evalúa las dos dimensiones del apego romántico adulto: la evitación (de la intimidad y la ansiedad (acerca de las relaciones. Los participantes fueron 549 adultos, quienes completaron el cuestionario ECR y el cuestionario de apego adulto CaMir. Se evaluó las propiedades psicométricas del cuestionario, mostrando que es un instrumento con una adecuada validez de criterio y con una estructura factorial que representa las dos dimensiones teóricas propuestas por los autores originales. Se concluye que la versión chilena del ECR es válida para examinar el apego romántico en la adultez.

  10. Better Rooting Procedure to Enhance Survival Rate of Field Grown Malaysian Eksotika Papaya Transformed with 1-Aminocyclopropane-1-Carboxylic Acid Oxidase Gene

    OpenAIRE

    Rogayah Sekeli; Janna Ong Abdullah; Parameswari Namasivayam; Pauziah Muda; Umi Kalsom Abu Bakar

    2013-01-01

    A high survival rate for transformed papaya plants when transferred to the field is useful in the quest for improving the commercial quality traits. We report in this paper an improved rooting method for the production of transformed Malaysian Eksotika papaya with high survival rate when transferred to the field. Shoots were regenerated from embryogenic calli transformed with antisense and RNAi constructs of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) genes using the Agrobacterium tum...

  11. Revista Chilena de Cirugía: índice de publicación según procedencia de los artículos / Summ affiliation of authors publishing in Revista Chilena de Cirugía

    Scientific Electronic Library Online (English)

    MANUEL, VIAL G; CARLOS, MANTEROLA D; VIVIANA, PINEDA N.

    2008-02-01

    Full Text Available Introducción: Una de las variables que se analiza en los estudios bibliométricos es la procedencia de las publicaciones. Este tipo de análisis ha permitido cuantificar el impacto que tienen las distintas universidades o centros hospitalarios en el total de publicaciones de una revista en particular. [...] El objetivo de este estudio es determinar una eventual asociación entre el índice de publicación y la procedencia de los artículos publicados en la Revista Chilena de Cirugía (Rev Chil Cir). Material y método: Estudio biblio-métrico. Se analizaron todos los artículos publicados en la Rev Chil Cir entre los años 2002 y 2006. Los artículos se categorizaron de acuerdo al año de publicación, el tipo de artículo, región del país que originó el artículo y la filiación universitaria manifestada en cada artículo. Se calculó la tasa de publicación, el promedio anual y general de publicación, y la tasa de publicación anual promedio (TPAP). Posteriormente, se aplicó estadística analítica para determinar diferencias entre grupos, utilizando T de Student, ANOVA y prueba de comparaciones múltiples de Duncan. Resultados: En el periodo estudiado, se publicaron 545 artículos científicos, con un promedio de 18,2 artículos por número y 109 artículos por volumen. El 67,9 % fue generado por la Región Metropolitana (RM), el 10,8 % por la VIII región y el 7,8 % por la IX región. Solo en un 64,8 % de las publicaciones queda de manifiesto alguna filiación universitaria. Las mayores TPAP correspondieron a los Capítulos IX y VIII Región con 2,3 + 0,8 y 1,8 + 0,2 publicaciones/año x 10 miembros (p Abstract in english Background: The analysis of author affiliation of published manuscripts allows an assessment of the impact that different universities or hospitals have on a determinate journal. Aim: To analyze author affiliation of manuscripts published in Revista Chilena de Cirugía. Material and methods: All manu [...] scripts published in the journal between 2002 and 2006, were analyzed. The affiliation of authors, the year of publication and the region of Chile in which the manuscript was generated, were recorded. The publication rate, annual average were calculated. The association of authors to specific regional subsidiaries of the Chilean Society of Surgeons was also recorded and the average annual publication rates per regional subsidiary, were calculated. Resu/fs; During the study period 545 manuscripts were published, with a mean of 18 manuscripts per issue and 109 per volume. Sixty eight percent of manuscripts came from the metropolitan region, 11 % from the eight region and 8 % from the ninth region. In only 65 % of manuscripts, authors had an university affiliation. The higher average annual publication rates were observed in the subsidiaries of the eighth and ninth region, with 2.3 + 0.8 and 1.8 + 0.2 publications per year per 10 members, respectively. Conclusions: An important proportion of manuscripts have authors without university affiliation. The higher average annual publication rates come from regional subsidiaries of the Chilean Society of Surgeons

  12. Promover la salud mental con mujeres chilenas en desventaja social / Promoting mental health in socially disadvantaged chilean women / Promover a saúde mental com mulheres chilenas em desvantagem social

    Scientific Electronic Library Online (English)

    M Soledad, Rivera; M Sylvia, Campos; Solange, Campos; Ilta, Lange; Mila, Urrutia; Angela, Castellano.

    2004-12-01

    Full Text Available Descreve-se uma experiência do projeto chileno-canadense, intitulado "Promover a saúde mental de mulheres chilenas em desvantagem social: os Segredos de Maruja". Apresentam-se os objetivos, as etapas das intervenções e as avaliações preliminares. Com a participação das mulheres usuárias dos Centros [...] de Saúde, profissionais de saúde e autoridades da comunidade de La Pintana, foi construído, aplicado e avaliado um projeto de intervenção para apoiar as mulheres na tomada de decisões que favorecessem sua saúde mental, pessoal, familiar e social. A intervenção contemplou o desenho, a produção, a distribuição e a avaliação de 12 cartilhas educativas tipo calendários, cada uma referente a três temas de saúde mental considerado importante pelas mesmas mulheres: identidade e auto-estima; vínculos e comunicação; e autocuidado. Cada cartilha tem um teste de autodiagnóstico e endereços dos recursos sociais comunitários. Ademais, comprometeu as enfermeiras que trabalham nos Centros de Saúde que incorporaram esta estratégia no seu atendimento habitual e avaliaram seu impacto tanto na sua identidade e autonomia profissional quanto na melhoria da qualidade do cuidado brindado às mulheres. Abstract in spanish Se descrive una parte de la experiencia del proyecto Chileno/Canadiense: "Promover la salud mental de las mujeres chilenas en desventaja social: Los Secretos de Maruja". Se describen los objetivos, las etapas del proceso, de intervención y los resultados de las evaluaciones preliminares. Con la part [...] icipación de las mujeres usuárias de los centros de salud, profesionales y autoridades de la comuna de La Pintana, se construyó, aplicó y evaluó un proyecto de intervención para apoyar a las mujeres en la toma de decisiones que favorezcan su salud mental personal, familiar y social. La intervención contempló el diseño, producción, distribución y evaluación de 12 folletos educativos tipo calendarios, cada uno respecto a tres temas de salud mental identificados por las mismas mujeres: Identidad y autoestima, Vínculo y Comunicación y Autocuidado. Además se involucró a las enfermeras que trabajan en los Centros de Salud, quienes incorporaron esta estrategia a la atención de salud habitual y evaluaron su impacto en la identidad, autonomía profesional y en la mejoría de la calidad del cuidado. Abstract in english This paper describes a Chilean/Canadian intervention project about Mental Health Promotion with socially disadvantaged women called "Maruja's secrets". We present their objectives, each stage of the intervention process and preliminary evaluations. With the participation of women of the community, h [...] ealth professionals and authorities of health members of La Pintana, we conceive, develop and evaluate an intervention to support and coach women in decision making to improve their personnel and social mental health. The intervention process include design, engaged community nurses, distribution and evaluation of 12 educative color calendars, each one with 14 messages about mental health issues, that were discovered in focus groups with the same women: Identity and self- esteem; communication and selfcare. Each calendar has 14 messages, a self-diagnosis test and addresses of community social resources. Community nurses were involved in the distribution and they evaluate their experience as a way of strengthening nursing identity and the quality of nursing care.

  13. The effect of gamma irradiation on the ripening degree of papaya by comparing the changes of colour index, texture firmness and pH value

    International Nuclear Information System (INIS)

    This study is made to see the effect of gamma radiation on papaya (carica papaya) from eksotika cultivar through colour index comparison, firmness and pH value. Dose used on fruit is 0.0 kGy, 0.5 kGy, 1.0 kGy. 1.5 kGy and 2.0 kGy. All fruit was wrapped by using plastic after fruit being radiated. Sample was stored at temperature room for 14 days and will be analysed in day 1, 3, 7, 10 and 14. It was found the dose 1.0 kGy show slowest papaya experience ripening process compared to the other dose. Through radiation found that radiation can extend further papaya life span. After day 10, found that for dose 0.0, 0.5, 1.5 and 2.0 kGy suffer rot. For dose 0.0, 0.5, 1.5 and 2.0 kGy, discovered it is not suitable with the papaya in this study. Even more cleary, radiation is good for delayed the ripening rate but it is must be suitable with the papaya. (author)

  14. Recuperation of the high germinability condition of papaya seed through priming technology and bioregulators / Recuperación de la alta capacidad de germinación de la semilla de papaya mediante la tecnología de preacondicionamiento y biorreguladores

    Scientific Electronic Library Online (English)

    Francisco, Bautista-Calles; Guillermo, Carrillo-Castañeda; Ángel, Villegas-Monter.

    2008-11-01

    Full Text Available La pérdida rápida de la viabilidad de la semilla de Carica papaya L. (papaya) y su elevado precio comercial afectan la disponibilidad de semillas de alta calidad para los cultivadores. Se uso semilla certificada de papaya cv. Maradol para determinar las metodologías prácticas para recuperar la capac [...] idad de germinación de las semillas. Las semillas se sometieron a: a) preacondicionamiento hí d rico: tras 4 d de preacondicionamiento hídrico la germinación de semillas aumentó hasta 84%, 250% más que en aquellas que no se trataron; sin embargo, las plántulas generadas a partir de las semillas no tratadas acumularon más biomasa que las que sí se trataron; 2) preacondicionamiento químico: las semillas tratadas durante 4 d en una solución de cloruro de calcio 10-5 M germinaron 262% más que las no tratadas y, además, sus plántulas acumularon más biomasa que las plántulas testigo. Cuando las semillas se trataron ya sea en solución de ácido salicílico 10-4 o en la de Agromil S®, se obtuvo una germinación significativa, 81 y 73% más alta que en las no tratadas. Todas las semillas tratadas germinaron con mayor rapidez que las no tratadas. Abstract in english Rapid loss of seed viability of Carica papaya L. (papaya) and its high commercial price impairs the availability of high quality seed to growers. Certified papaya seed cv. Maradol was utilized to define practical methodologies to restore the seed germination performance. Seed was submitted to: 1) hy [...] dropriming: 4 d of hydropriming rose seed germination up to 84%, 250% more than the untreated seeds; however, the seedlings generated from untreated seeds accumulated more biomass than the other treatments; 2) chemopriming: seeds treated 4 d in a 10-5 M calcium chloride solution germinated 262% more than did the untreated seeds and in addition, the seedlings generated from treated seeds accumulated more biomass than the control seedlings. When seeds were treated either in the 10-4 M salicylic acid or in Agromil S® solution high germination was accomplished 81 and 73% higher than the untreated seed respectively. All treated seeds germinated faster than the untreated ones.

  15. Effect of irradiation on color of minimally processed melon and papaya

    International Nuclear Information System (INIS)

    The access to nutritious food is an essential dimension of food meal. High potential for fresh-cut industry exists and ready-to-eat fruit market has grown rapidly in recent years due to the health benefits associated. Although there is many concerns to food safety other parameters like texture, taste, color and sensory acceptance are fundamental principles of acceptance to any food. Actually the use of instrumental measurements has proven to be a major predictor of sensory responses. According to many authors, the addition of different techniques should always be considered to provide additional information of the sensory aspects. Therefore the objective of this study was to evaluate the influence of irradiation on color of minimally processed melon and papaya. The fruits were purchased in a market of Sao Paulo, at the same point of ripeness and sent to the IPEN/CNEN-SP. The fruits were sanitized and manually cut into cubes of approximately 2 x 2 cm with the aid of stainless steel knives and packed in polyethylene bags. Melons and papaya were irradiated in a Multipurpose Gamma Source (IPEN - Sao Paulo - Brazil) and were divided in six groups for color analysis: Control; 0.5 kGy; 1.0 kGy, 1.5kGy, 2.0 kGy and 3.0kGy. After the treatment, the MP fruits were kept in a refrigerator at 4 deg C ± 1 deg C until the end of the analysis. The color of the samples was determined using a Minolta colorimeter CR-400 Chromameter. The parameters L*, a* and b* were evaluated. The results were statistically analyzed by analysis of variance One-Dimensional Analysis of Variance (One-Way-ANOVA) followed by Tukey test. All statistical analysis was performed using the program Graph Pad Prism 5 and adopting a significance level of 5 % (p* parameter of any dose despite the tendency to darkening observed for the group of 3.0 kGy. This fact also occurred for the chromatographic coordinates a* and b* which remained in the same tonality for all treatments (p<0.05). Current results indicate that gamma radiation can be used for keeping the color. Doses up to 3kGy were efficient for that. Future studies should consider pretreatments to minimize the darkening effects on fruits. Therefore, the results from this study suggest the use of gamma radiation as a quality alternative for keep the color of minimally processed melon and papaya. (author)

  16. Efecto del calcio en aplicaciones precosecha y poscosecha sobre la severidad de antracnosis (Colletotrichum gloeosporioides y la calidad de frutos de papaya (Carica papaya

    Directory of Open Access Journals (Sweden)

    Daniel Sabor\\u00EDo

    2000-01-01

    Full Text Available Se evalu6 el efecto de aplicaciones pre- cosecha y poscosecha de calcio en papaya va- riedad "criolla" sabre la severidad de antracno- sis (CoUetotrichum gloeosporioides y varia- hIes de calidad del fruto. Los tratamientos pre- cosecha fueron 4: aspersi6n de CaCl2 al 1% Y 4% (2 aplicaciones: <40 dfas posantesis (dpa y entre 100-140 dpa con el penetrante alquilaril- polimero (NP- 7 Bayer (0.4 mIlL, CaCO3 al suelo (1 ton/ha, 70 dpa y testigo (0% Ca. El diseno experimental fue un BCA (4 repeticio- nes de 20 frutos. Los tratamientos poscosecha fueron 3: inmersiones par 5 min con 0%, 1 % Y 4% de CaCI2, con el mismo penetrante. El dise- no experimental fue un BCA (3 repeticiones de 15 frutas. Se evalu6 severidad, % calcio en cascara, brix, pH, % acidez, firmeza (cascara y pulpa y % de madurez. En la aplicaci6n de cal- cia precosecha la severidad fue: 1 % CaC12 con 6%, testigo 7%, CaCO3 9% y 4% CaC12 con 11 %, no se encontr6 que el Ca tuviera un efec-

  17. Dois novos sistemas de diagnose precoce da meleira do mamoeiro / Two new systems of early diagnosis of papaya sticky disease

    Scientific Electronic Library Online (English)

    Eder T., Tavares; Joseli S., Tatagiba; José A., Ventura; Manoel T., Souza Jr..

    2004-10-01

    Full Text Available A diagnose da meleira do mamoeiro (Carica papaya)tem sido feita mediante observação de sintomas que aparecem principalmente nos frutos ou pela detecção de dsRNA de cerca de 12 kb, purificado a partir do látex em coluna de CF11, em gel de agarose ou poliacrilamida. Os sintomas nos frutos são tardios [...] e permitem longa permanência de plantas infectadas no campo e o processo usado para detectar dsRNA é laborioso, não se prestando a detecção em larga escala. Visando disponibilizar protocolos de diagnose precoce da meleira que possam ser usados em larga escala, o presente trabalho apresenta dois métodos de detecção relativamente baratos, rápidos e que permitem o manuseio de dezenas de amostras por dia. Os dois métodos utilizam o látex extraído dos frutos, folhas ou caule de plantas, e se baseiam na extração e visualização de ácidos nucléicos. A presença do vírus é confirmada pela visualização do seu dsRNA em gel de agarose (1%) em 1X TBE. Com esta técnica temos conseguido resultados confiáveis a ponto de diagnosticar precocemente a meleira em plantas ainda jovens e em plantas assintomáticas. Abstract in english Papaya sticky disease was first reported affecting papaya (Carica papaya) in Brazil in the late 80s. Today this disease is found in the papaya production areas throughout Brazil, and in some of them it became the main limiting factor for the papaya industry. The primary disease symptom is an excessi [...] ve exudation of highly fluid latex that becomes dark as result of oxidation and turns the fruit unmarketable. It is caused by a new virus that has an isometric particle (40-50 nm in diameter), and a unique 12 kb long dsRNA molecule. Since its diagnosis is done mainly by observation of the symptoms on the fruit, infected plants may be source of inoculum for several months before diagnosis. Another form of diagnosis is the detection of dsRNA from leaves and latex using CF11 columns. This is a laborious system not suited to large scale usage. The present work presents two cheap and fast diagnostic protocols. These protocols use latex obtained from fruits, leaves and stems, and are based on the extraction and visualization of nucleic acids. Presence of the virus is confirmed by the visualization of dsRNA on agarose (1%) gel in 1X TBE. Using these protocols it is possible to confirm the presence of the virus in young and assymptomatic plants.

  18. La solidez de la economía chilena a la luz de las reformas estructurales: 1970-1997

    Directory of Open Access Journals (Sweden)

    Ángeles SÁNCHEZ DÍEZ

    2009-11-01

    Full Text Available RESUMEN: En el presente artículo se recogen las principales reformas estructurales que han tenido lugar en Chile desde 1970 y que constituyen la base de la solidez actual de dicha economía latinoamericana. Se propone una división en períodos en la que se distingue la época del gobierno de la Unidad Popular, el gobierno de la Junta Militar y la transición a la democracia para evaluar los resultados económicos que se registraron a la luz de las distintas estrategias de desarrollo. Las principales reformas que se dieron en el periodo 1970-1973 bajo la "vía chilena hacia el socialismo" fueron la creación del Área de Propiedad social, la profundización de la Reforma Agraria y la nacionalización de la Gran Minería Durante el monetarismo ortodoxo (1974-1982 destacan como principales reformas la liberalización y desregulación de los mercados, la l a ronda de privatizaciones, la apertura comercial, la liberalización financiera y las "siete modernizaciones", todas ellas respaldadas por la ortodoxia de la denominada Escuela de Chicago. Tras la profunda crisis de 1982 que afectó a todo el continente latinoamericano fue necesario replantear el modelo y se introdujeron distintas medidas de carácter neoliberal que consiguieron que fuera posible aprovechar los beneficios de las reformas realizadas con anterioridad. La transición a la democracia constituye un reto para la sociedad chilena, en tanto que contando con las bases de una economía sólida deben incorporarse la visión social y política para que el crecimiento se convierta en desarrollo. En este sentido toma importancia la última estrategia de la CEPAL de "Transformación productiva con equidad".ABSTRACT: In this paper, the main structural reforms are searched, which happened between 1970 and 1995. These are the base of the actual economic framework. The different considered periods are: the "Unidad Popular" government, the military government and the democratic transition. Finally, we must evaluate the economic results that are dependent of the implementation of the reforms and of the different development strategies. The main reforms that occurred in 1970-1973 with the "vía chilena hacia el socialismo" were: The creation of "area de propiedad social" —social property area-, the agrarian reform and the nationalisation of the most important mining companies. In the orthodox monetarism (1974-1982, the liheralisation and deregulation of the markets, the 1" Stage/Round of privatisation, the trade and the financial liberation and the seven modernizations were the most outstanding reforms, which were supported by the Chicago School. The heavy crisis which affected Latin- American countries and also Chile, caused the reforms of monetarism made possible the improvement of the effect of the reforms of the Chicago Boys. Finally the transition to democracy was am important challenge to Chilean society because Chile had the bases of a solid economy, but it was necessary to attend social and political issues to change growth into development. For this reason the last strategy of the ECLAC "Transformación productiva con equidad" is so important.

  19. Effects of papaya leaves on thrombocyte counts in dengue--a case report.

    Science.gov (United States)

    Siddique, Osama; Sundus, Ayesha; Ibrahim, Mohammad Faisal

    2014-03-01

    Dengue fever is on the rise in developing nations like India, Pakistan, Sri Lanka and Bangladesh. There is no antiviral chemotherapy or vaccine for dengue virus and management of the disease is done on supportive measures. The decline in the thrombocyte count leads to dengue haemorrhagic fever accounting for complications and mortality. Oral administration of Carica papaya leaves extract is said to have a positive impact on thrombocyte count. A 23-year-old man was administered a calculated dose for five days. Blood samples were tested for complete blood count before and after the administration of the juice. Thrombocyte count had increased from 28000/micro liter to 138000/micro liter at the end of five days. We present our experience here. PMID:24864622

  20. Preparation and characterization of inclusion complex of benzyl isothiocyanate extracted from papaya seed with ?-cyclodextrin.

    Science.gov (United States)

    Li, Wenzhao; Liu, Xiaoyu; Yang, Qingfeng; Zhang, Ning; Du, Yideng; Zhu, Huaping

    2015-10-01

    The inclusion complex of benzyl isothiocyanate (BITC), extracted from papaya seed with ?-cyclodextrin (?-CD), was prepared. Different analytical techniques, such as Fourier transform infrared spectroscopy, thermal analysis, X-ray diffractometry, particle size distribution analysis and (1)H Nuclear magnetic resonance analysis, were used to investigate the characterization of the inclusion complex (BITC-?-CD). All these approaches indicated that the inclusion complex was capable of being formed. The inclusion complex exhibited different spectroscopic and thermodynamic features and properties from BITC, and we deduced the possible inclusion modes for BITC-?-CD. The calculated apparent stability constant of the BITC-?-CD was 600.8l/mol, and the aqueous solubility of BITC was indistinctively improved by phase solubility studies. The results illustrated that ?-CD was a proper excipient for increasing the stability and controlled release of BITC. Thus, ?-CD complexation technology would be a promising approach, in expanding the application of BITC as a food antibacterial agent. PMID:25872431

  1. Effect of irradiation on color of minimally processed melon and papaya

    Energy Technology Data Exchange (ETDEWEB)

    Fabbri, Adriana D.T.; Sagretti, Juliana M.A.; Hirashima, Fabiana K.; Rogovschi, Vladimir D.; Nunes, Thaise C.F.; Sabato, Susy F., E-mail: adriana.fabbri@yahoo.com.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil). Lab. de Irradiacao de Alimentos

    2013-07-01

    The access to nutritious food is an essential dimension of food meal. High potential for fresh-cut industry exists and ready-to-eat fruit market has grown rapidly in recent years due to the health benefits associated. Although there is many concerns to food safety other parameters like texture, taste, color and sensory acceptance are fundamental principles of acceptance to any food. Actually the use of instrumental measurements has proven to be a major predictor of sensory responses. According to many authors, the addition of different techniques should always be considered to provide additional information of the sensory aspects. Therefore the objective of this study was to evaluate the influence of irradiation on color of minimally processed melon and papaya. The fruits were purchased in a market of Sao Paulo, at the same point of ripeness and sent to the IPEN/CNEN-SP. The fruits were sanitized and manually cut into cubes of approximately 2 x 2 cm with the aid of stainless steel knives and packed in polyethylene bags. Melons and papaya were irradiated in a Multipurpose Gamma Source (IPEN - Sao Paulo - Brazil) and were divided in six groups for color analysis: Control; 0.5 kGy; 1.0 kGy, 1.5kGy, 2.0 kGy and 3.0kGy. After the treatment, the MP fruits were kept in a refrigerator at 4 deg C ± 1 deg C until the end of the analysis. The color of the samples was determined using a Minolta colorimeter CR-400 Chromameter. The parameters L{sup *}, a{sup *} and b{sup *} were evaluated. The results were statistically analyzed by analysis of variance One-Dimensional Analysis of Variance (One-Way-ANOVA) followed by Tukey test. All statistical analysis was performed using the program Graph Pad Prism 5 and adopting a significance level of 5 % (p<0.05), expressed as the mean results ± standard deviation. Samples of papaya and melons showed no statistical difference for the L{sup *} parameter of any dose despite the tendency to darkening observed for the group of 3.0 kGy. This fact also occurred for the chromatographic coordinates a{sup *} and b{sup *} which remained in the same tonality for all treatments (p<0.05). Current results indicate that gamma radiation can be used for keeping the color. Doses up to 3kGy were efficient for that. Future studies should consider pretreatments to minimize the darkening effects on fruits. Therefore, the results from this study suggest the use of gamma radiation as a quality alternative for keep the color of minimally processed melon and papaya. (author)

  2. Phytochemical and antifungal profiles of the seeds of Carica papaya L.

    Directory of Open Access Journals (Sweden)

    Onkar Singh

    2011-01-01

    Full Text Available Phytochemical investigation of the ethanolic extracts of the seeds of Carica papaya L. (Caricaceae led to the isolation of 2,3,4-trihydroxytoluene (caricaphenyl triol and glyceryl-1-(2?,3?,4?-trihydroxybenzoyl-2,3-dioleate (papayaglyceride as the new phytoconstituents along with the known components glyceryl-1-oleiyl-2,3-dilinoleiate, glyceryl-1-oleiyl-2,3-diarachidate, glyceryl-1-linoleiyl-2,3-distearate, carpaine, glyceryl-1,2-dipalmitate, glyceryl trimyristate, glyceryl tristearate, glyceryl-1,2-dipalmityl-3-myristate, glyceryl-1-oleiyl-2,3-dimyristate, ?-sitosterol glucoside, glyceryl-1-oleiyl-3-phosphate, glyceryl-1-oleiyl-2-lauryl-3-phosphate and glyceryl-1,2-distearyl-3-phosphate. The structures of all these compounds have been elucidated by spectral data analysis and chemical reactions. The methanolic extract of the seeds and 2,3,4-trihydroxytoluene (200 ?g/ml showed antifungal activity against Aspergillus flavus, Candida albicans and Penicillium citrinium.

  3. Process variables influence on microwave assisted extraction of pectin from waste Carcia papaya L. peel.

    Science.gov (United States)

    Maran, J Prakash; Prakash, K Arun

    2015-02-01

    The objectives of the present study were to evaluate and optimize the influence of process variables such as microwave power, pH, time and solid-liquid ratio on the extraction of pectin from waste Carcia papaya L peel. The experiments were carried out based on a four factors three level Box-Behnken response surface design. A quadratic model was developed from the experimental data in order to predict the pectin yield. The optimal condition was found to be: microwave power of 512 w, pH of 1.8, time of 140 s and solid-liquid ratio of 1:15 g/ml with maximum pectin yield (25.41%). PMID:25445679

  4. Evaluación de la uniformidad del riego y efecto del fertirriego nitrogenado en un huerto de lechosa (Carica papaya L. Evaluation of irrigation uniformity and effect of nitrogen fertirrigation in a papaya orchard (Carica papaya L.

    Directory of Open Access Journals (Sweden)

    Carmen Basso

    2008-08-01

    Full Text Available En un huerto de lechosa cv. Cartagena Roja se estudió el desempeño del fertirriego nitrogenado y riego sobre el rendimiento del cultivo. Se evaluaron cuatro tratamientos de fertilización nitrogenada y tres de riego, utilizando cintas de goteo, y se registró la humedad del suelo usando un TDR y mediante gravimetría. La cosecha se inició al final del período lluvioso y se prolongó durante veinte y un semanas del período seco. Se encontró que la descarga de los emisores resultó altamente uniforme en sus distintas modalidades de operación. Los tratamientos de fertilización nitrogenada no se vieron reflejados en el rendimiento de las plantas pero sí en el adelanto de la cosecha en los tratamientos con mayor dosis de fertilizante. Los tratamientos de riego tampoco afectaron los rendimientos, lo cual se atribuyó al efecto de la humedad almacenada en el perfil durante el período húmedo.Nitrogenous fertilization and watering performance on yield of a papaya orchard cv. Red Cartagena was studied. Four treatments of nitrogenous fertilization and three of watering were evaluated using drip lines. The soil moisture was registered by TDR and gravimetry. The harvest began at the end of the rainy period and it prolonged for twenty one weeks in the dry period. It was found that the emitter discharge was highly uniform in its different operation modalities. The nitrogenous fertilization did not affect the plant yield but permitted earlier maturity of fruits. Similarly, the watering treatments did not affect the yield which was attributed to the effect of the water stored in the soil profile during the rainy period.

  5. Evaluación de la uniformidad del riego y efecto del fertirriego nitrogenado en un huerto de lechosa (Carica papaya L.) / Evaluation of irrigation uniformity and effect of nitrogen fertirrigation in a papaya orchard (Carica papaya L.)

    Scientific Electronic Library Online (English)

    Carmen, Basso; Roberto, Villafañe; Stalin, Torres; José, Díaz.

    2008-08-01

    Full Text Available En un huerto de lechosa cv. Cartagena Roja se estudió el desempeño del fertirriego nitrogenado y riego sobre el rendimiento del cultivo. Se evaluaron cuatro tratamientos de fertilización nitrogenada y tres de riego, utilizando cintas de goteo, y se registró la humedad del suelo usando un TDR y media [...] nte gravimetría. La cosecha se inició al final del período lluvioso y se prolongó durante veinte y un semanas del período seco. Se encontró que la descarga de los emisores resultó altamente uniforme en sus distintas modalidades de operación. Los tratamientos de fertilización nitrogenada no se vieron reflejados en el rendimiento de las plantas pero sí en el adelanto de la cosecha en los tratamientos con mayor dosis de fertilizante. Los tratamientos de riego tampoco afectaron los rendimientos, lo cual se atribuyó al efecto de la humedad almacenada en el perfil durante el período húmedo. Abstract in english Nitrogenous fertilization and watering performance on yield of a papaya orchard cv. Red Cartagena was studied. Four treatments of nitrogenous fertilization and three of watering were evaluated using drip lines. The soil moisture was registered by TDR and gravimetry. The harvest began at the end of t [...] he rainy period and it prolonged for twenty one weeks in the dry period. It was found that the emitter discharge was highly uniform in its different operation modalities. The nitrogenous fertilization did not affect the plant yield but permitted earlier maturity of fruits. Similarly, the watering treatments did not affect the yield which was attributed to the effect of the water stored in the soil profile during the rainy period.

  6. Germinação e vigor de sementes de mamão (Carica papaya L.) cv. Golden secadas em altas temperaturas / Germination and vigour of high-temperature dried seeds of Golden papaya (Carica papaya L.)

    Scientific Electronic Library Online (English)

    Vinicius de Oliveira, Carlesso; Pedro Amorim, Berbert; Roberto Ferreira da, Silva; José Tarcísio Lima, Thiébaut; Marcia Terezinha Ramos de, Oliveira.

    Full Text Available O presente estudo teve como objetivo avaliar os efeitos imediato e latente do emprego de altas temperaturas de secagem na qualidade fisiológica das sementes de mamão cv. Golden. Os testes de secagem por convecção, em camada delgada, foram realizados a 50 e 58ºC, empregando-se fluxo de ar seco de 1,0 [...] 0kg s-1 m-2; as sementes foram armazenadas a 15ºC, por 90 e 180 dias, em embalagens herméticas. A secagem a 50 e 58ºC teve efeito imediato negativo sobre o vigor das sementes de mamão. Quanto ao efeito latente, apenas as sementes secadas a 58ºC e armazenadas por 180 dias mantêm o mesmo vigor observado em sementes recém retiradas dos frutos. Em relação ao percentual de germinação, as sementes secadas a 50ºC e avaliadas imediatamente depois da secagem são influenciadas negativamente pela secagem. Abstract in english This study investigates the immediate and latent effects of high drying-air temperatures on the physiological quality of Golden papaya seeds. Thin-layer convective drying tests were conducted employing two levels of drying-air temperature, 50 and 58ºC, and a single level of dry-airflow rate, 1.00kg [...] s-1 m-2. Upon removal from the dryer, seeds were hermetically stored at 15ºC for 90 and 180 days. High drying-air temperatures had an immediate deleterious effect on seed vigour. Analysis of the latent effects revealed that best vigour results were obtained for seeds dried at 58ºC and stored for 180 days at 15ºC. Germination percentage immediately after drying was reduced for seeds dried at 50ºC only. Germination of the seeds submitted to other treatments was not affected by drying-air temperature or storage period, when compared to the values obtained for fresh seeds.

  7. Germinação e vigor de sementes de mamão (Carica papaya L. cv. Golden secadas em altas temperaturas Germination and vigour of high-temperature dried seeds of Golden papaya (Carica papaya L.

    Directory of Open Access Journals (Sweden)

    Vinicius de Oliveira Carlesso

    2009-01-01

    Full Text Available O presente estudo teve como objetivo avaliar os efeitos imediato e latente do emprego de altas temperaturas de secagem na qualidade fisiológica das sementes de mamão cv. Golden. Os testes de secagem por convecção, em camada delgada, foram realizados a 50 e 58ºC, empregando-se fluxo de ar seco de 1,00kg s-1 m-2; as sementes foram armazenadas a 15ºC, por 90 e 180 dias, em embalagens herméticas. A secagem a 50 e 58ºC teve efeito imediato negativo sobre o vigor das sementes de mamão. Quanto ao efeito latente, apenas as sementes secadas a 58ºC e armazenadas por 180 dias mantêm o mesmo vigor observado em sementes recém retiradas dos frutos. Em relação ao percentual de germinação, as sementes secadas a 50ºC e avaliadas imediatamente depois da secagem são influenciadas negativamente pela secagem.This study investigates the immediate and latent effects of high drying-air temperatures on the physiological quality of Golden papaya seeds. Thin-layer convective drying tests were conducted employing two levels of drying-air temperature, 50 and 58ºC, and a single level of dry-airflow rate, 1.00kg s-1 m-2. Upon removal from the dryer, seeds were hermetically stored at 15ºC for 90 and 180 days. High drying-air temperatures had an immediate deleterious effect on seed vigour. Analysis of the latent effects revealed that best vigour results were obtained for seeds dried at 58ºC and stored for 180 days at 15ºC. Germination percentage immediately after drying was reduced for seeds dried at 50ºC only. Germination of the seeds submitted to other treatments was not affected by drying-air temperature or storage period, when compared to the values obtained for fresh seeds.

  8. First record of Trialeurodes variabilis (Quaintance) (Hemiptera: Aleyrodidae) on Carica papaya L. in the state of Espírito Santo, Brazil / Primeiro registro de Trialeurodes variabilis (Quaintance) (Hemiptera: Aleyrodidae) em Carica papaya L. no Espírito Santo

    Scientific Electronic Library Online (English)

    Mark P., Culik; David dos S., Martins.

    2004-10-01

    Full Text Available Foram realizados levantamentos para identificar as moscas-brancas que ocorrem em mamoeiro no estado do Espírito Santo. É registrada pela primeira vez a presença da espécie de mosca-branca Trialeurodes variabilis (Quaintance) no Espírito Santo, em mamoeiro de seis localidades dos municípios de Sooret [...] ama e Linhares nos períodos de outubro a dezembro de 2002 e junho de 2003. Abstract in english Surveys were conducted to identify whitefly pests of papaya in the state of Espírito Santo, Brazil. The whitefly species Trialeurodes variabilis (Quaintance) is reported for the first time in the state, where it was found on papaya at six locations in Sooretama and Linhares municipalities in October [...] to December 2002 and June 2003.

  9. Inducción in vitro de raíces de Carica papaya mediante Agrobacterium rhizogenes y ácido 3-indolbutírico / In vitro induction of Carica papaya roots through Agrobacterium rhizogenes and 3-indolebutyric acid

    Scientific Electronic Library Online (English)

    Manuel de Jesús, Bermúdez Guzmán; Pedro, Valadez Ramírez; Marco Tulio, Buenrostro Nava; Gilberto, Manzo Sánchez; Salvador, Guzmán González.

    2013-11-01

    Full Text Available El enraizamiento de plántulas in vitro es una de las etapas más importantes en el proceso de micropropagación y en C. papaya ha sido una de las principales limitantes, obteniéndose porcentajes de sobrevivencia inferiores al 50%. El presente trabajo de investigación se realizó en el laboratorio de Bi [...] otecnología de la Universidad de Colima en 2009. Con el propósito de inducir un sistema radicular eficiente, se evaluaron brotes de papaya var. "Maradol" de aproximadamente 3 cm de longitud que fueron inoculados con A. rhizogenes a concentraciones de 1x10(7), 1x10(8) y 1x10(9) células mL-1 y establecidos en medio MS con acetosiringona (100 mM) y floroglucinol (25 mg/L). Para el experimento con la auxina, los brotes se subcultivaron en medio líquido MS con 0, 1, 2, 3, 4 y 5 ?M de AIB; se incubaron en oscuridad durante 126 h, seguido del subcultivo en sustrato BM2+sales minerales del MS. Ninguno de los brotes inoculados con las concentraciones bacterianas formó raíces; en contraste, 100% de los brotes tratados con AIB a una concentración de 3 ?M formaron raíces. Con lo anterior se demuestra que las plántulas de C. papaya posiblemente no son susceptibles a la cepa A4 de A. rhizogenes, mientras que el empleo de AIB resultó ser la opción más viable y efectiva para la inducción in vitro de raíces en brotes de papaya. Abstract in english In vitro rooting of seedlings is one of the most important steps in the process o f micropropagatio n and in C. Papaya has been one of the major limiting, having survival percentages below 50%. The present research was conducted in the laboratory of Biotechnology of the University of Colima in 2009. [...] In order to induce an efficient root system, evaluated papaya shoots var. "Maradol" of approximately 3 cm length, inoculated with A. rhizogenes at concentrations of 1x10(7), 1x10(8) and 1x10(9) cells ml-1 and established on MS medium with acetosyringone (1 00 mM) and phloroglucinol (25 mg / L). For the experiment with auxin shoots were subcultured in MS liquid medium containing 0, 1, 2, 3, 4 and 5 ?M IBA; incubated in the dark for 126 h, followed by subculture in substrate BM2 + MS mineral salts. None of the inoculated shoots with bacterial concentrations formed roots; in contrast, 100% of the treated shoots with IBA to a concentration of 3 ?M formed roots. With the above demonstrates that seedlings of C. papaya may not be susceptible to strain A4 A. rhizogenes, while the use of IBA proved to be the most viable and effective option for in vitro root induction in shoots of papaya.

  10. Nitrogen fertilization sources and insecticidal activity of aqueous seeds extract of Carica papaya against Spodoptera frugiperda in maize / Fuentes nitrogenadas de fertilización y actividad insecticida del extracto acuoso de semilla de Carica papaya contra Spodoptera frugiperda en maíz

    Scientific Electronic Library Online (English)

    Rodolfo, Figueroa-Brito; Patricia, Villa-Ayala; Jesús F, López-Olguín; Arturo, Huerta-de la Peña; Juan R, Pacheco-Aguilar; Miguel A, Ramos-López.

    2013-12-01

    Full Text Available R. Figueroa-Brito, P. Villa-Ayala, J.F. López-Olguín, A. Huerta-de la Peña, J.R. Pacheco-Aguilar y M.A. Ramos-López. 2013. Fuentes nitrogenadas de fertilización y actividad insecticida del extracto acuoso de semilla de Carica papaya contra Spodoptera frugiperda en maíz. Cien. Inv. Agr. 40(3): 567-57 [...] 7. Se estimó el daño causado por el gusano cogollero del maíz Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) durante la etapa vegetativa de maíz cultivado con fertilizantes nitrogenados químicos, vermicomposta, y extracto acuoso de semillas de Carica papaya L. (Caricaceae). Cada brote se infestó con una larva de primer instar de S. frugiperda. Las variables evaluadas incluyen el porcentaje de germinación. Las variables respuesta fueron el porcentaje de emergencia, longitud de la segunda y quinta hoja, diámetro de tallo y altura de la planta; así como la estimación de daño causado por larvas de S. frugiperda al maíz. Los resultados indicaron que la vermicomposta ayudó a la emergencia de la semilla, en una relación 3:1 de suelo con vermicomposta alcanzó 100% de emergencia, mientras que solo con suelo, la emergencia fue de 80%; el sulfato de amonio incrementó el tamaño de la segunda hoja y quinta hoja 89,6 y 160,4% respectivamente, aumentó el diámetro de tallo y la altura de la planta 290,2 y 13,3%, respecto al tratamiento donde solo se aplicó agua. Las fuentes de nitrógeno estimularon que S. frugiperda ocasionara más daño a la planta, el tratamiento con urea presentó 70% de daño, con fosfonitrato 62,3%, con sulfato de amonio fue de 51,8% sin extracto acuoso de semillas de C. papaya. El tratamiento con sulfato de amonio + extracto acuoso de semillas de C. papaya registró el menor daño del insecto al maíz con 29,6%. Abstract in english R. Figueroa-Brito, P. Villa-Ayala, J.F. López-Olguín, A. Huerta-de la Pena, J.R. Pacheco-Aguilar, and M.A. Ramos-López. 2013. Nitrogen fertilization sources and insecticidal activity of aqueous seeds extract of Carica papaya against Spodoptera frugiperda in maize. Cien. Inv. Agr. 40(3): 567-577. The [...] damage caused by the fall armyworm Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) to vegetative-stage maize cultivated with chemical nitrogen fertilizers, vermicompost, and Carica papaya L. (Caricaceae) seed extract was estimated. Each shoot was infested with one first instar larva of S. frugiperda. The variables measured included the percentage of germination, length of the second and fifth leaves, stem diameter and plant height, and the estimation of damage caused by S. frugiperda larvae on maize. The results indicated that vermicompost helped seed germination on a relation 3:1 from black soil and vermicompost, the emergence was 100%, while the emergence with black soil was 80%. The ammonium sulfate increased the length of the second and fifth leaves 89.6% and 160.4% respectively, augmented the stem diameter and the plant height 290.2% and 13.3% respectively, respect to water treatment. The exogenous nitrogen sources stimulate S. frugiperda to cause more damage to the plant, the treatment urea showed 70% of damage, with phosphonitrate 62.3% and with ammonium sulfate 51.8%, when were evaluated without aqueous seed extract of C. papaya. Ammonium sulfate + aqueous extract of C. papaya seeds showed the lowest insect damage to maize with 29.6%.

  11. Lactogenic Activity of an Enzymatic Hydrolysate from Octopus vulgaris and Carica papaya in SD Rats.

    Science.gov (United States)

    Cai, Bingna; Chen, Hua; Sun, Han; Sun, Huili; Wan, Peng; Chen, Deke; Pan, Jianyu

    2015-11-01

    The traditional Chinese medicine theory believes that octopus papaya soup can stimulate milk production in lactating women. The objective of this study was to determine whether dietary supplementation with an enzymatic hydrolysate of Octopus vulgaris and Carica papaya (EHOC) could increase milk production and nutritional indexes in Sprague Dawley (SD) rats. Female SD rats (n?=?24) were fed a control diet (n?=?8), EHOC-supplemented diet, or a positive control diet (Shengruzhi) from day 10 of pregnancy to day 10 of lactation. Maternal serum, mammary gland (day 10 of lactation), milk, and pup weight (daily) were collected for analysis. Results showed that the EHOC diet obviously elevated daily milk yield and pup weight compared to the control group (P?diet was found to increase the concentration of prolactin (PRL), progesterone (P), estradiol (E2), and growth hormone (GH) significantly in the circulation and mammary gland. Mammary glands of EHOC-treated dams showed clear lobuloalveolar development and proliferation of myoepithelial cells, but no striking variations were observed among the groups. Furthermore, the nutrition content and immune globulin concentration in the milk of EHOC-supplemented dams were higher than those of the control group, especially the cholesterol, glucose, and IgG were higher by 44.98% (P?

  12. Improved Immunoassay Sensitivity in Serum as a Result of Polymer-Entrapped Quantum Dots: 'Papaya Particles'.

    Science.gov (United States)

    Ranzoni, Andrea; den Hamer, Anniek; Karoli, Tomislav; Buechler, Joseph; Cooper, Matthew A

    2015-06-16

    Fluorescent labels are widely employed in biomarker quantification and diagnostics, however they possess narrow Stokes shifts and can photobleach, limiting multiplexed detection applications and compromising sensitivity. In contrast, quantum dots do not photobleach and have much wider Stokes shifts, but a paucity of robust surface attachment chemistries for bioconjugation has limited their uptake in biomedical diagnostics. We report a novel class of biofunctional fluorescent labels based on trapping of ∼10(4) quantum dots within a core nanoparticle. The doped particles act as scaffolds for generation of a multilayered shell consisting of a functionalized hydrophilic polymer with covalently attached receptors for analyte capture. These constructs, which conceptually resemble a papaya fruit, are chemically stable, remain monodispersed for >6 months in buffer, and show utility in immunoassay applications. Using monoclonal antibody fragments against nonstructural protein dengue NS1, an early biomarker for dengue fever, antibody immobilization capacity was 75-fold higher compared with traditional carbodiimide protein coupling. In the model dengue immunoassay, we observed a 15-fold lower limit of detection and 4-fold higher fluorescence intensity with the "papaya particles" compared to current "best-in-class" commercial reagents. Direct deployment in human serum allowed sensitive detection of different NS1 serotypes with lower limits of detection within the clinically relevant range (1-10 ng/mL), and sufficient specificity for identification of the dengue serotype was achieved for concentrations >10 ng/mL (DV1-3) and >50 ng/mL (DV4). The combination of chemical and physical stability and high binding capacity combined with the intrinsic advantages of quantum dots may enable more simple, robust diagnostic assays in the future. PMID:25971296

  13. Protective Effect of Carica papaya Linn Against gamma-Radiation-Induced Tissue Damage in Rats

    International Nuclear Information System (INIS)

    The present study was designed to determine the possible protective effects of the Carica papaya fruit aqueous extract (CP) against ?-radiation induced oxidative stress, biochemical and hematological alterations in male albino rats. Papaya (250 mg/Kg BW /day) was given to male albino rats, via gavages for 6 days prior exposure to the 1st radiation fraction and the treatment was continued for 14 days after the 1st irradiation fraction till the end of the experiment (4 Gy / week up to 8 Gy total doses). The samples were taken from the blood and some organs, liver and kidney for the biochemical analysis. In the irradiated group, there were a significant decrease in RBCs, WBCs count and Hb content. Dramatic increments in the serum indices of liver (aspartate transaminase, alanine transaminase, alkaline phosphatase and bilirubin) and kidney (urea, uric acid and creatinine) functions were also recorded depicting a liver and kidney impairment state. Also, a significant increase in thiobarbituric acid reactive substances (TBARS) content and Xanthine oxidase (XO) activity in parallel to a significant decrease in the activity of xanthine dehydrogenase accompanied by a significant decrease in reduced glutathione content (GSH), superoxide dismutase (SOD), catalase (CAT) activities were recorded in both liver and kidney tissues compared to control group. Treatment with CP (250 mg/kg) was found to offer significant protection against gamma-radiation induced toxicity in the tissues, which was evident by the improved status of most of the parameters investigated. These results suggest that CP could increase the antioxidant defense systems in the liver and kidney of irradiated animals, and may protect from adverse effects of whole body radiation

  14. GROWTH OF MACROBRACHIUM ROSENBERGII FED WITH MANGO SEED KERNEL, BANANA PEEL AND PAPAYA PEEL INCORPORATED FEEDS

    Directory of Open Access Journals (Sweden)

    P. Aarumugam

    2013-06-01

    Full Text Available The growth promoting potential of fruits wastes, mango seed kernel, banana peel and papaya peel on the freshwater prawn, Macrobrachium rosenbergii post larvae (PL was evaluated. Basal diet equated to 35% protein was prepared by using soybean meal, groundnut oilcake, horse gram and wheat flour. Each fruit waste powder was separately incorporated with basal diet at a proportion of 10%. Sunflower oil was used as lipid source. Egg albumin and tapioca flour were used as binding agents. Vitamin B-complex with Vitamin-C was also mixed. Feed without any fruit waste was served as control. M. rosenbergii PL (length: 1.2-1.4 cm; weight: 0.09- 0.13 g was fed with these feeds for a period of 90 days. Significant improvements in the nutritional indices (survival rate, weight gain, biomass index, specific growth rate and condition factor, concentrations of biochemical constituents (total protein, carbohydrate and lipid, levels of non-enzymatic antioxidants (vitamin-C and E, content of minerals (Na+ and K+, activities of digestive enzymes (protease, amylase and lipase, and profiles of essential amino acids and fatty acids were recorded in fruits wastes incorporated feeds fed PL when compared with control (P< 0.003 – 0.878. The overall results indicated the fact that mango seed kernel incorporated feed was produced the best performance, followed by better performance of banana peel and good performance of papaya peel. These fruits wastes incorporated feeds enhance digestive enzymes activities and act as appetizer, which in turn enhances food utilization and ultimately yielded better survival and growth of M. rosenbergii PL. Therefore, these fruits wastes have considerable potentials in sustainable development of Macrobrachium culture.

  15. La Generación Messenger: Relevancia de la mensajería instantánea en la adolescencia chilena

    Scientific Electronic Library Online (English)

    Luis, Cárcamo Ulloa; Felipe, Nesbet Montecinos.

    2008-08-01

    Full Text Available Em meio às mudanças que geraram a chamada Era da Informação, o fenômeno da Comunicação Mediada por Computador (cmc) ganha importância. Isto diz respeito ao crescente acesso da sociedade chilena à internet, o que a posiciona como líder na América Latina. Considerando a familiaridade dos jovens com as [...] novas tecnologias, este grupo etéreo é o que se apropria com maior intensidade desta nova ferramenta comunicativa. A mensagem instantânea passa a ser uma importante ferramenta para os processos de socialização dos jovens, ocupando o lugar que em décadas passadas era do telefone. Diferente de outras tecnologias, a mensagem instantânea constitui um dos poucos elementos comuns que atravessa os segmentos sociais e as diversas tribos urbanas, pela qual os jovens chilenos vivem seus processos de construção de identidade. Abstract in spanish En medio de los cambios que ha generado la llamada Era de la Información, adquiere importancia el fenómeno de la Comunicación Mediada por Computador (CMC). Esto se relaciona con el creciente acceso de la sociedad chilena hacia la internet, que lo posiciona como líder en Latinoamérica en el tema. Dad [...] a la cercanía que los jóvenes tienen con las nuevas tecnologías, este grupo etáreo es el que se apropia con mayor intensidad de esta nueva herramienta comunicativa. La mensajería instantánea pasa a ser una importante herramienta para los procesos de socialización de los jóvenes, ocupando el lugar que en décadas pasadas tuvo el teléfono. A diferencia de otras tecnologías, la mensajería se convierte en uno de los pocos elementos comunes que cruza los segmentos sociales y las variadas tribus urbanas en las cuales los jóvenes chilenos viven sus procesos de construcción de la identidad. Abstract in english In the middle of changes has generated by Age of Information called, acquired importance the phenomenon of Computer Mediated Communication (cmc). This is related to the increasing access that the Chilean society towards the internet, that positions it like leader in Latin America in the subject. By [...] the proximity that the young people have with new technologies, this age group has been has itself appropriate with greater intensity of this new communicative tool. The instant messaging has become an important tool by he socialization processes of the young people, having occupied the place that in last decades use the telephone. Compared to other technologies the mail has become one of the few common elements that it crosses the social segments and the varied urban tribes in who the Chilean young people live their processes on construction of the identity.

  16. Low-dose gamma irradiation following hot Water immersion of Papaya (Carica Papaya linn.) fruits provides additional control of postharvest fungal infection to extend shelf life

    DEFF Research Database (Denmark)

    Rashid, M.H.A.; Grout, Brian William Wilson; Continella, A.; Mahmud, T.M.M.

    2015-01-01

    Low-dose gamma irradiation (0.08 kGy over 10 min), a level significantly below that required to satisfy the majority of international quarantine regulations, has been employed to provide a significant reduction in visible fungal infection on papaya fruit surfaces. This is appropriate for local and national markets in producer countries where levels of commercial acceptability can be retained despite surface lesions due to fungal infection. Irradiation alone and in combination with hot-water imme...

  17. Low-dose gamma irradiation following hot Water immersion of Papaya (Carica Papaya linn.) fruits provides additional control of postharvest fungal infection to extend shelf life

    DEFF Research Database (Denmark)

    Rashid, M.H.A.; Grout, Brian William Wilson

    2015-01-01

    Low-dose gamma irradiation (0.08 kGy over 10 min), a level significantly below that required to satisfy the majority of international quarantine regulations, has been employed to provide a significant reduction in visible fungal infection on papaya fruit surfaces. This is appropriate for local and national markets in producer countries where levels of commercial acceptability can be retained despite surface lesions due to fungal infection. Irradiation alone and in combination with hot-water immersion (50 °C for 10 min) has been applied to papaya (Carica papaya L.) fruits at both the mature green and 1/3 yellow stages of maturity. The incidence and severity of surface fungal infections, including anthracnose, were significantly reduced by the combined treatment compared to irradiation or hot water treatment alone, extending storage at 11 °C by 13 days and retaining commercial acceptability. The combined treatment had no significant, negative impact on ripening, with quality characteristics such as surface and internal colour change, firmness, soluble solids, acidity and vitamin C maintained at acceptable levels.

  18. QUALITATIVE PHYTOCHEMICAL SCREENING AND ANTIFUNGAL ACTIVITY OF CARICA PAPAYA LEAF EXTRACT AGAINST HUMAN AND PLANT PATHOGENIC FUNGI

    Directory of Open Access Journals (Sweden)

    Sikandar Khan Sherwani

    2013-07-01

    Full Text Available Plants have been explored extensively all over the globe in quest of a novel bioactive compound that could a good therapeutic candidate treating infectious diseases especially against drug resistant microbes. Qualitative phytochemical analyses of Carica papaya leaf extract reveal that except steroids and tannins all the possible phytochemical constituents including carbohydrates, proteins, anthraquinones, flavonoids, saponins, cardiac glycosides and alkaloids were present. Two ways of Carica papaya leaf extract preparations i.e crushed and boiled were tested for their antifungal activity against 6 saprophytic fungi Penicillium sp, Aspergilus flavus, Aspergillus niger, Fusarium sp, Rhizopus and Helminthosporum, 5 dermatophytic fungi Microsporum canis, Microsporum gypseum, Trichophyton rubrum, Trichophyton mentagrophytes, Trichophyton tonsurans and 6 yeasts including Candida albicans, Candida albicans ATCC 0383, Saccharomyces cerevisiae, Candida galbrata, Candida tropicalis, Candida kruzei. The activity was found against majority of fungi but was much better in case of crushed leaf extract.

  19. Binary Combination of Carica papaya, Areca catechu and Myristica fragrans with Piperonyl Butoxide / MGK-264 against Freshwater Snail Lymnaea acuminata

    Science.gov (United States)

    Hanif, Farhat; Singh, Dinesh Kumar

    2013-01-01

    Piperonyl butoxide (PB) and MGK-264 were used to enhance the toxicity of the active components papain, arecoline and myristicin from the plants Carica papaya, Areca catechu and Myristica fragrans, respectively, against the vector snail Lymnaea acuminata. A time- and dose-dependent relationship was observed for the toxicity of these combinations. The toxic effects of these plant-derived molluscicides in combination with the synergists PB and MGK-264 were several times higher than the effect of the individual treatments. The highest degree of synergism was observed when MGK-264 was used in combination with C. papaya latex (10.47-fold increase) and PB was used with papain (8.35-fold increase). PMID:24575245

  20. Victimización y heroísmo. Disputas de las memorias emblemáticas de la izquierda chilena en dos fechas conmemorativas: aniversario del golpe de Estado y día del joven combatiente

    Directory of Open Access Journals (Sweden)

    Tamara vidaurrazaga aránguiz

    2015-01-01

    Full Text Available En el presente trabajo proponemos reflexionar en torno a dos memorias emblemáticas y dicotómicas que tienen lugar dentro de la izquierda chilena a la hora de recordar a quienes resistieron a la dictadura de Pinochet, reelaboraciones que hemos denominado memoria de la victimización y memoria del heroísmo, y que ejemplificaremos en dos fechas icónicas para la izquierda chilena: la conmemoración del Golpe de Estado cada 11 de septiembre, y el Día del Joven Combatiente rememorado cada 29 de marzo. Lo que nos interesa es reflexionar en torno a la marmolización que implican estas dos reconstrucciones, y la dificultad para imaginar un futuro para el proyecto político heredado por la izquierda chilena al sumirnos en esta dicotomía estanca.

  1. Preliminary In Vitro Antisickilng Properties of Crude Juice Extracts of Persia Americana, Citrus Sinensis, Carica Papaya and Ciklavit®

    OpenAIRE

    Iweala, EE J; Uhegbu, FO; Ogu, GN

    2009-01-01

    The antisickling properties of crude juice extracts of the edible portions of three commonly consumed tropical fruits namely Persia americana, Citrus sinensis, and Carica papaya were investigated in vitro alongside a new drug preparation called Ciklavit® that has antisickling activity. Four different solvent extracts of the crude juice of each fruit including aqueous, acidic, alkaline and alcoholic extracts were prepared and their antisickling effects on sickle cell trait (HbAS) and sickle ce...

  2. QUALITATIVE PHYTOCHEMICAL SCREENING AND ANTIFUNGAL ACTIVITY OF CARICA PAPAYA LEAF EXTRACT AGAINST HUMAN AND PLANT PATHOGENIC FUNGI

    OpenAIRE

    Sikandar Khan Sherwani,; Tasveer Zahra Bokhari; Kanwal Nazim; Syed Aneel Gilani; Shahana Urooj Kazmi

    2013-01-01

    Plants have been explored extensively all over the globe in quest of a novel bioactive compound that could a good therapeutic candidate treating infectious diseases especially against drug resistant microbes. Qualitative phytochemical analyses of Carica papaya leaf extract reveal that except steroids and tannins all the possible phytochemical constituents including carbohydrates, proteins, anthraquinones, flavonoids, saponins, cardiac glycosides and alkaloids were present. Two ways of Carica ...

  3. Acute and Chronic Hepatotoxicity Study of Orally Administered Ethanol Extract of Carica papaya Seeds in Adult Wistar Rats

    Directory of Open Access Journals (Sweden)

    Wilson O. Hamman

    Full Text Available Carica papaya seed extracts have shown great promise in the quest for the development of natural plant based male contraceptive. This study investigates the acute and chronic hepatotoxicity of ethanol extract of C. papaya seeds in adult Wistar rats. The OECD up-and-down method was used in the acute oral toxicity test using 5 nulliparous, nonpregnant female wistar rats weighing between 180 and 200 g and observed for 14 days. The chronic hepatotoxicity study involved the use of 15 male wistar rats which were divided into 3 groups of 5 each. Group I which served as control were orally administered distilled water while groups II and III received 100 and 250 mg/kg/day of ethanol extract of C. papaya seeds respectively for a period of 90 days. Histological investigation of the liver and serum levels of AST, ALT and ALP were carried out at the end of the administration. Result obtained for the acute oral toxicity showed no mortality or morbidity. There was no significant loss of fur and skin lesions, nose and eyes appeared clear and normal. There was no diarrhea, convulsion, salivation, tremors, lethargy, sleep or coma and animals did not show any sign of aggression or unusual behavior during handling. For the chronic hepatotoxicity study, the histology of the liver, hematological indices and serum levels of AST, ALT and ALP showed no significant difference between the control and experimental groups. The study thus concludes that ethanol extract of C. papaya seeds is non-toxic and safe.

  4. Binary Combination of Carica papaya, Areca catechu and Myristica fragrans with Piperonyl Butoxide / MGK-264 against Freshwater Snail Lymnaea acuminata

    OpenAIRE

    Hanif, Farhat; SINGH, Dinesh Kumar

    2013-01-01

    Piperonyl butoxide (PB) and MGK-264 were used to enhance the toxicity of the active components papain, arecoline and myristicin from the plants Carica papaya, Areca catechu and Myristica fragrans, respectively, against the vector snail Lymnaea acuminata. A time- and dose-dependent relationship was observed for the toxicity of these combinations. The toxic effects of these plant-derived molluscicides in combination with the synergists PB and MGK-264 were several times higher than the effect of...

  5. [Parasitism in Trialeurodes variabilis (Quaintance) (Hemiptera: Aleyrodidae) by Encarsia hispida De Santis (Hymenoptera: Aphelinidae), in papaya, in Brazil].

    Science.gov (United States)

    Lourenção, André L; Fancelli, Marilene; Costa, Valmir A; Ribeiro, Nicolle C

    2007-01-01

    Infestation of Trialeurodes variabilis (Quaintance) was observed in October 2004, in papaya plants of cultivar Sunrise Solo, under screenhouse conditions, in Cruz das Almas, State of Bahia, Brazil. In infested leaves, around 20% of parasitism on nymphs was verified. Leaves with parasitized nymphs were kept in laboratory until emergence of the parasitoid, identified as Encarsia hispida De Santis. This is the first time that this parasitoid was detected on T. variabilis nymphs in Brazil. PMID:17420874

  6. Induction of the expression of defence genes in Carica papaya fruit by methyl jasmonate and low temperature treatments

    Scientific Electronic Library Online (English)

    Marisela, Rivera-Domínguez; Karen Rosalinda, Astorga-Cienfuegos; Martin Ernesto, Tiznado-Hernández; Gustavo Adolfo, González-Aguilar.

    2012-09-15

    Full Text Available The defence mechanisms that are activated by methyl jasmonate (MJ) in fruits are not well understood. In this work, we studied the expression of defence genes in papaya fruit that are induced by the exposure to MJ and/or low temperatures. The papaya fruits ?Maradol? were randomly divided into two gr [...] oups: one group was the untreated control and the other was treated with 10-4 M of MJ. Half of the fruits from each of the two groups were stored after treatment for 5 days at 5ºC and 2 days at 20ºC. We studied the expression levels of the pdf1.1 and pdf1.2 genes by amplification from expression libraries created from the pulp and skin tissues of the papaya fruit. As a reference, the mRNA level of the 18s ribosomal gene was used. In the skin tissue, the expression levels of the pdf1.1 and pdf1.2 genes were higher immediately after MJ treatment compared to the control. Furthermore, the expression of pdf1.2 remained high after MJ treatment and subsequent storage compared to the control. It was therefore concluded that the activation of the pdf1.1 and pdf1.2 genes forms part of the molecular defence mechanism in fruits that is activated by exposure to MJ. To our knowledge, this is the first study that analyzes the gene expression in papaya fruit that is induced by the exogenous application of methyl jasmonate and cold treatment.

  7. Autoritarismo, enajenación y locura en la poesía chilena de fines del siglo XX. Zurita, Maquieira, Cuevas.

    Directory of Open Access Journals (Sweden)

    Óscar GALINDO VILLARROEL

    2009-11-01

    Full Text Available RESUMEN: El ensayo analiza la figura del sujeto en tres poetas claves de la poesía chilena de fines del siglo XX: Raúl Zurita, Diego Maquieira y José Ángel Cuevas, con el propósito de dar cuenta del proceso de enajenación vivido por diversos tipos de subjetividades en crisis. Este complejo proceso de metaforización de la censura y la represión social se traduce en una multiplicidad de voces que interactúan en el espacio del texto, dando lugar a voces desquiciadas, en los márgenes entre la razón y la locura, que complejizan las relaciones entre escritura y vida. Así la ambigüedad sexual, el disfraz, la escisión del sujeto, son rasgos de una escritura de una fuerte carga testimonial y crítica.ABSTRACT: The essay analyzes the figure of the subject in three of the most important Chilean poets at the end twentieth century, Raúl Zurita, Diego Maquieira and José Ángel Cuevas, in orden to show the process of alienation lived by different types of subject in situations of crisis. The complex processes of censorship and social repression converted to metaphors appears in the form of multiple voices interacting in the textual space, deranged voices on the frontiers of reason and madness which complicate the relation between writing and life. Sexual ambiguity, the mask, and the fragmentation of the subject are characteristics of this highly testimonial and critical literature.

  8. La Asociación Chilena de Editores de Revistas Biomédicas The Chilean Association of Biomedical Journal Editors

    Directory of Open Access Journals (Sweden)

    Humberto Reyes B

    2001-01-01

    Full Text Available On September 29th, 2000, The Chilean Association of Biomedical Journal Editors was founded, sponsored by the "Comisión Nacional de Investigación Científica y Tecnológica (CONICYT" (the Governmental Agency promoting and funding scientific research and technological development in Chile and the "Sociedad Médica de Santiago" (Chilean Society of Internal Medicine. The Association adopted the goals of the World Association of Medical Editors (WAME and therefore it will foster "cooperation and communication among Editors of Chilean biomedical journals; to improve editorial standards, to promote professionalism in medical editing through education, self-criticism and self-regulation; and to encourage research on the principles and practice of medical editing". Twenty nine journals covering a closely similar number of different biomedical sciences, medical specialties, veterinary, dentistry and nursing, became Founding Members of the Association. A Governing Board was elected: President: Humberto Reyes, M.D. (Editor, Revista Médica de Chile; Vice-President: Mariano del Sol, M.D. (Editor, Revista Chilena de Anatomía; Secretary: Anna María Prat (CONICYT; Councilors: Manuel Krauskopff, Ph.D. (Editor, Biological Research and Maritza Rahal, M.D. (Editor, Revista de Otorrinolaringología y Cirugía de Cabeza y Cuello. The Association will organize a Symposium on Biomedical Journal Editing and will spread information stimulating Chilean biomedical journals to become indexed in international databases and in SciELO-Chile, the main Chilean scientific website (www.scielo.cl (Rev Méd Chile 2001;129: 95-98

  9. Prevalencia de vaginosis bacteriana en trabajadoras sexuales chilenas / Prevalence of bacterial vaginosis in Chilean sex workers

    Scientific Electronic Library Online (English)

    Gloria, Venegas; Gioconda, Boggiano; Erica, Castro.

    2011-07-01

    Full Text Available OBJETIVO: Determinar la prevalencia de vaginosis bacteriana (VB) en trabajadoras sexuales chilenas y relacionar los hallazgos con variables sociodemográficas, sexuales y clínicas. MÉTODO: Se estudió una muestra de 379 trabajadoras sexuales que asistían para control a Unidades de Atención y Control d [...] e Salud Sexual de Chile. A todas se las entrevistó para obtener antecedentes sociodemográficos y sexuales, se les realizó evaluación clínica que incluyó características del flujo vaginal, pH y prueba de aminas, y se les tomó una muestra vaginal para tinción de Gram. Para el diagnóstico de VB se empleó el criterio de Nugent. Los datos fueron analizados con EPI-INFO 3.4.1 y BioStat, utilizándose un grado de significación de P Abstract in english OBJECTIVE: To determine the prevalence of bacterial vaginosis (BV) in Chilean sex workers and relate the findings to sociodemographic, sexual, and clinical variables. METHODS: A sample of 379 sex workers seen in Chilean Sexual Health Monitoring and Care Units for check-ups was studied. All of them w [...] ere interviewed to obtain their sociodemographic and sexual history. A clinical examination was performed that included the characteristics of vaginal discharge, pH, and amine test. A vaginal sample was taken for Gram stain. The Nugent criteria were used for the diagnosis of BV. The data was analyzed with EPI-INFO 3.4.1 and BioStat, using a degree of significance of P

  10. Verbo de concordancia en la lengua de señas chilena

    Directory of Open Access Journals (Sweden)

    Dora Adamo Quintela

    1999-09-01

    Full Text Available El presente trabajo trata sobre una clase verbo de la lengua de señas chilena, el verbo de concordancia. A través de su descripción se da cuenta de los diversos mecanismos utilizados por el sistema de la lengua de señas para establecer la concordancia entre el sujeto y el objeto, que funcionan en la oración mediante el proceso de inflexión gramatical. Una característica relevante de la LSCh consiste en la utilización del espacio para producir modificaciones gramaticales regulares, entendidas como procesos de inflexión, a través de la co-ocurrencia de parámetros gestual-visuales, que permiten una comunicación eficiente entre sus usuarios. (This article focuses on a particular type of verb used in Chilean Sign Language: the verb of agreement. Through its description, different mechanisms used to show subject-object accord operating through inflection are presented. A relevant feature of Chilean Sign Language (LSCh, is to use space to mark regular grammatical modifications, understood as processes of inflection through the co-occurrence of visual-kinetic parameters that allow its user an efficient communication.

  11. La construcción discursiva de los imaginarios sociales: el caso de la medicina popular chilena

    Directory of Open Access Journals (Sweden)

    Gerardo Álvarez

    2002-09-01

    Full Text Available En este artículo se da cuenta del estado de avance de una investigación interdisciplinaria (lingüística, psicología, sociología sobre las representaciones de cuerpo (salud/enfermedad en la medicina popular chilena (Proyecto FONDECYT 1000376. No se trata de una investigación sobre la medicina como tal. El objetivo es rastrear el imaginario sobre el cuerpo que subyace a las prácticas curativas y que se revela a través del discurso de las sanadoras populares (mapuches y no mapuches, y luego examinar las relaciones que se establecen entre estos dos tipos de saberes: el saber médico científico "occidental" y los saberes tradicionales populares. El método de análisis deriva de la teoría de la enunciación, de la polifonía enunciativa y del análisis actancialde los relatos. (This article deals with the representation of the human body (in terms of health and sickness which underlies the curative practices of popular Chilean "curanderas" and "meicas" (Research project: FONDECYT 1000376. Through an interdisciplinary approach –linguistic, psychological and sociological– the authors examine the imagery of the human body that is revealed through discoursive practices, and the relationship between the scientific "western" medical representations and those of popular medicine women (mapuche and "hispanic". The analysis of interviews draws mainly upon the theories of enunciation (Benveniste, intertextuality (Bakhtin and the actantial analysis of tales (Greimas.

  12. Poder, Ideología y el Consenso de Washington: Desarrollo y Expansión de la Política Chilena de Vivienda.

    Directory of Open Access Journals (Sweden)

    Alan Gilbert

    2003-01-01

    Full Text Available Es bien sabido que a menudo la realidad supera la ficción. Esto ciertamente se aplica en el caso del modelo de subsidio habitacional en Chile. Desarrollado por los chilenos con ayuda de la ideología neo-liberal inventada en Chicago, las poderosas instituciones de Washington DC aparecen como actores menores en la escena chilena. Inicialmente, Chile necesitaba el apoyo y financiamiento del Banco Mundial pero una vez conseguidos, Chile siguió su propia agenda. El Banco de Desarrollo Interamericano y USAID no lograron mucho más. Chile era el amo y señor en su propia casa. ¿Fue ésta una victoria para la autonomía nacional por sobre el poder del financiamiento internacional?. Ciertamente no, porque el nuevo modelo que se estaba aplicando en Chile fue el precursor de mucho de lo que se estaba implementando desde Washington en los días de ajuste estructural. Chile ?ganó? porque aceptó las reglas del nuevo juego establecido por instituciones muchísimo más poderosas que los bancos de desarrollo multilaterales. Washington del Desarrollo aprendió mucho de Chile y luego aplicó lo aprendido a los países más endeudados, más pequeños y menos sofisticados.

  13. UN GRAN VAR BAYESIANO PARA LA ECONOMIA CHILENA / LARGE BAYESIAN VAR FOR THE CHILEAN ECONOMY

    Scientific Electronic Library Online (English)

    WILDO, GONZALEZ P.

    2012-10-01

    Full Text Available Este artículo desarrolla un gran VAR bayesiano con más de cien variables para la economía chilena, en el mismo sentido que Banbura, Giannone y Reichlin (2010) se muestra que cuando el grado de contracción del ajuste de los priors son fijados en relación a la dimensión del corte transversal de la mue [...] stra (bayesian shrinkage), la capacidad predictiva de un VAR puede ser mejorado agregando variables macroeconómicas e información sectorial. Los resultados muestran que la predicción del gran VAR bayesiano se compara favorablemente con relación a algunos modelos univariados. Se examinan adicionalmente los impulsos respuesta a un shock monetario, así como también de algunos shocks sectoriales. Abstract in english This article develops a Large Bayesian VAR with more than 100 variables for the Chilean economy, as Banbura, Giannone and Reichlin (2010) shows that, when the degree of shrinkage is set in relation to the cross-sectional dimension of the sample (bayesian shrinkage), the forecasting performance of a [...] VAR can be improved by adding macroeconomic variables and sectoral information. The results show that the large bayesian VAR compares favorably with some univariate models. It further examines the impulse response functions to a monetary shock, as well as some sectoral shocks.

  14. Regiones extremas chilenas y su invisibilidad económica / Economic invisibility of Chile’s end regions

    Scientific Electronic Library Online (English)

    Sergio, Soza-Amigo; Loreto, Correa.

    2014-12-01

    Full Text Available En este artículo se constata que las zonas extremas de Chile no poseen una estructura que viabilice un modelo de desarrollo homogéneo respecto a las demás regiones, y que dichas diferencias se manifiestan en sus composiciones demográficas y económicas. La hipótesis central de este trabajo es que las [...] regiones extremas del norte y sur-austral son prácticamente invisibles en el contexto económico nacional. Además, se advierte a través de un análisis input-output, que en el desarrollo endógeno de ambas regiones extremas, Arica y Parinacota no es capaz de generar recursos para satisfacer sus propias necesidades de desarrollo, y que Magallanes y Antártica Chilena al menos ha construido su base económica de manera más formal y diversificada. Abstract in english This article verifies that the end regions of Chile do not have a structure that makes viable a homogeneous development model in relationship to the rest of the regions, and these differences are manifested in their demographic and economic compositions. The central hypothesis of this article is tha [...] t the end regions in the north and the extreme south are practically invisible in the national economic context. The proposed article notes that, in analyzing the endogenous development of both regions, in the north Arica and Parinacota are not able to generate resources to meet their own development needs, and in the south the Magellan and Chilean Antarctica regions have at least built their economic base in a more formal and diversified manner.

  15. Crecimiento, convergencia y espacio en las regiones chilenas: 1960-1998.

    Directory of Open Access Journals (Sweden)

    Patricio Aroca

    2000-01-01

    Full Text Available El objetivo de este trabajo es investigar la popular hipótesis de convergencia en el proceso de crecimiento económico en Chile. Usando herramientas de la econometría espacial, replicamos la mayoría de los resultados de la literatura previa sobre el tema para Chile. Sin embargo, encontramos nueva evidencia que muestra que en la última década, la de mayor crecimiento del país, el proceso de crecimiento no ha sido convergente, desde el punto de vista de cohesión regional. Los resultados revelan una alta y creciente concentración espacial del PIBpc regional. Además, dos ?clusters? espaciales regionales son detectados para esa misma variable, indicando que el desarrollo económico de Chile puede estar segmentando espacialmente el país en regiones altamente productivas y dinámicas (Regiones I, II y III y otras regiones que van paulatinamente quedando más rezagadas (Regiones VIII, IX y X. Las estimaciones de las tradicionales ecuaciones de convergencia apoyan la tesis de que en los años 90, un período de fuerte crecimiento económico, se puede descartar la idea de ?-convergencia y que muy difícilmente se puede mantener la hipótesis de que las regiones chilenas se dirijan hacia una progresiva igualación de su producto per cápita. Adicionalmente, las estimaciones realizadas presentan, para ese período, síntomas de una fuerte correlación espacial, con lo que se estaría confirmando la idea de que existen ciertos componentes del crecimiento regional que son compartidos por regiones que se encuentran próximas entre sí.

  16. La Asociación Chilena de Editores de Revistas Biomédicas / The Chilean Association of Biomedical Journal Editors

    Scientific Electronic Library Online (English)

    Humberto, Reyes B.

    2001-01-01

    Full Text Available [...] Abstract in english On September 29th, 2000, The Chilean Association of Biomedical Journal Editors was founded, sponsored by the "Comisión Nacional de Investigación Científica y Tecnológica (CONICYT)" (the Governmental Agency promoting and funding scientific research and technological development in Chile) and the "Soc [...] iedad Médica de Santiago" (Chilean Society of Internal Medicine). The Association adopted the goals of the World Association of Medical Editors (WAME) and therefore it will foster "cooperation and communication among Editors of Chilean biomedical journals; to improve editorial standards, to promote professionalism in medical editing through education, self-criticism and self-regulation; and to encourage research on the principles and practice of medical editing". Twenty nine journals covering a closely similar number of different biomedical sciences, medical specialties, veterinary, dentistry and nursing, became Founding Members of the Association. A Governing Board was elected: President: Humberto Reyes, M.D. (Editor, Revista Médica de Chile); Vice-President: Mariano del Sol, M.D. (Editor, Revista Chilena de Anatomía); Secretary: Anna María Prat (CONICYT); Councilors: Manuel Krauskopff, Ph.D. (Editor, Biological Research) and Maritza Rahal, M.D. (Editor, Revista de Otorrinolaringología y Cirugía de Cabeza y Cuello). The Association will organize a Symposium on Biomedical Journal Editing and will spread information stimulating Chilean biomedical journals to become indexed in international databases and in SciELO-Chile, the main Chilean scientific website (www.scielo.cl) (Rev Méd Chile 2001;129: 95-98)

  17. MONOPSONISTIC BEHAVIOR IN CHILEAN MANUFACTURING / COMPORTAMIENTO MONOPSONICO EN LA MANUFACTURA CHILENA

    Scientific Electronic Library Online (English)

    DAVID, MACKINSON; LUCAS, NAVARRO.

    2013-10-01

    Full Text Available Este trabajo utiliza una metodología firma-específica desarrollada por Brummund (2012) para medir poder monopsónico en el mercado de trabajo usando datos de plantas manufactureras chilenas para el período 2001-2006. Los resultados muestran una alta heterogeneidad en poder de mercado laboral entre la [...] s plantas y que cerca de un 25% de las plantas tienen un nivel alto de poder monopsónico. Se encuentra también que las características individuales de las plantas tienen un mayor poder explicativo del comportamiento monopsónico de las plantas que las características agregadas del mercado laboral en el que se desempeñan. La implicancia de política de estos resultados es que un salario mínimo restrictivo podría tener efectos ambiguos sobre el bienestar. Abstract in english This paper uses a firm-specific method for measuring monopsonistic behavior developed by Brummund (2012) using data for Chilean manufacturing plants for the period 2001-2006. We find that there is significant heterogeneity in labor market power across plants and that nearly a quarter of the plants h [...] ave a significant level of labor market power. It is also shown that individual plant characteristics explain more of the variation in monopsonistic behavior than do the characteristics of the accompanying labor market. These results are relevant for labor market policy since and suggest that a binding minimum wage could have ambiguous effects on welfare.

  18. Notas sobre ecocrítica y poesía chilena / Notes on ecocriticism and Chilean poetry

    Scientific Electronic Library Online (English)

    Mauricio, Ostria González.

    Full Text Available Se examinan algunas prácticas poéticas chilenas, caracterizadas por presentar visiones profundas de los sujetos humanos y la naturaleza. Se indaga en ellos tanto las relaciones de los sujetos con su medio y la presencia de una conciencia ecológica activa, como la plasmación discursiva de un imaginar [...] io vinculado a esa conciencia relacional: la plas-mación de vivencias de profunda integración del ser humano con el cosmos. Se indaga tanto los referentes ambientales como la articulación de elementos de la naturaleza en tanto expresión de los sujetos textuales. Abstract in english Certain Chilean poetics practices are examined, which present profound visions of human subjects and of nature. The inquiry focuses on both the subjects' relations with their environment, and the presence of an active ecological awareness, as well as the literary concretion of an imagery linked to t [...] hat relational awareness: the concretion of experiences of a profound integration of the human being with the cosmos. Environ-mental referents and the articulation of natural elements as expression of textual subjects are approached within the frame of globalization processes and the defense of regional cultural values.

  19. Estudiantes de sectores rurales en las universidades chilenas: problemas y desafíos

    Scientific Electronic Library Online (English)

    Juan, Cornejo Espejo.

    2013-12-01

    Full Text Available Si bien no existen estudios específicos acerca de los estudiantes provenientes de sectores rurales que ingresan a la educación superior en Chile ni de sus trayectorias académicas, se puede afirmar, a partir de otros estudios, que ha habido un fuerte aumento de este tipo de estudiantes en las distint [...] as universidades chilenas en la última década. Pese a esa expansión, su futuro académico y laboral resulta incierto en razón de las altas tasas de reprobación y abandonos tempranos, situación que obliga al diseño de una política de retención y apoyo académico-humano específica. Abstract in english Though specific studies do not exist about students from rural sectors who join the higher education institutions in Chile nor their academic paths, it is possible to affirm, from other studies, that there has been a strong increase of this type of students in the different Chilean universities in t [...] he last decade. In spite of this expansion, their academic and labor future turns out to be uncertain in reason of the high rates of failure and early abandons, situation that needs the design of retention politics, and human-academic funding.

  20. An analysis of the performance of chilean aquacultural exports. (1995-2005) / Análise do comportamento das exportações aqüícolas chilenas (1995-2005) / Análisis del comportamiento de las exportaciones acuícolas chilenas (1995-2005)

    Scientific Electronic Library Online (English)

    Cristian, Morales; Ramón, Lacayo; Rodrigo, Sfeir.

    2008-12-01

    Full Text Available As políticas cambiárias e de livre mercado introduzidas pelos últimos governos do Chile com o objetivo expresso de promover as exportações de produtos tradicionais e não tradicionais, têm tido como conseqüência, em particular, um relativo auge da aqüicultura. O cultivo do salmão é de renome, e expor [...] tações menos conhecidas deste setor têm uma importância cada vez maior no comportamento global da economia chilena. O objetivo deste artigo é a análise do comportamento das exportações aqüícolas chilenas para o período 1995-2005, como complemento de um trabalho anterior sobre as exportações agrícolas chilenas. Analisou-se o comportamento de vários indicadores de especialização (vantagem comparativa revelada e contribuição à balança comercial), estrutura de comercio (participação da exportação dos produtos selecionados no total das exportações) e competitividade (índices de participação setorial e de quota de mercado) em cada categoria exportada. Onze categorias do Sistema Harmonizado foram reduzidas a seis: salmão, filete de peixe, algas, ostras, mexilhões e invertebrados aquáticos. Além disso, se analisou o comportamento do principal país exportador e competidor de Chile em cada categoria selecionada. Os resultados permitem concluir que, exceções aparte, as exportações aqüícolas tanto de Chile como de seus principais competidores contam com um relativamente alto grau de especialização. Também mostram tendências na competitividade que podem ajudar a tomar medidas corretivas onde necessárias. Estes resultados podem ser úteis para os responsáveis na implementação de políticas com beneficio para o sector e para a economia em geral. Abstract in spanish Las políticas cambiarias y de libre mercado introducidas por los últimos gobiernos de Chile con el objetivo expreso de promover las exportaciones de productos tradicionales y no tradicionales, han tenido como consecuencia, en particular, un relativo auge de la acuicultura. El cultivo del salmón es d [...] e renombre, y exportaciones menos conocidas de este sector tienen una importancia cada vez mayor en el comportamiento global de la economía chilena. El objetivo de este artículo es el análisis del comportamiento de las exportaciones acuícolas chilenas para el periodo 1995-2005, como complemento de un trabajo anterior sobre las exportaciones agrícolas chilenas. Se analizó el comportamiento de varios indicadores de especialización (ventaja comparativa revelada y contribución a la balanza comercial), estructura de comercio (participación de la exportación de los productos seleccionados en el total de las exportaciones) y competitividad (índices de participación sectorial y de cuota de mercado) en cada categoría exportada. Once categorías del Sistema Armonizado fueron reducidas a seis: salmón, filete de pescado, algas, ostiones, mejillones e invertebrados acuáticos. Además, se analizó el comportamiento del principal país exportador y competidor de Chile en cada categoría seleccionada. Los resultados permiten concluir que, excepciones aparte, las exportaciones acuícolas tanto de Chile como de sus principales competidores gozan de un relativamente alto grado de especialización. También muestran tendencias en la competitividad que pueden ayudar a tomar medidas correctivas donde se necesite. Estos resultados pueden ser útiles a aquellos responsables de implementar políticas con beneficio para el sector y para la economía en general. Abstract in english Exchange rate and market liberalization policies put in place by recent Chilean administrations with the stated goal of promoting exports in both traditional and non-traditional sectors have resulted in a relative boom for the aquacultural activities in particular. The farming of salmon is well know [...] n, and less traditional exports in this area are beginning to play a larger role in the performance of the Chilean economy as a whole. This paper’s objective is the analysis of the performance of the Chilean aquacultural

  1. The component of Carica papaya seed toxic to A. aegypti and the identification of tegupain, the enzyme that generates it.

    Science.gov (United States)

    Nunes, Natalia N dos S; Santana, Lucimeire A; Sampaio, Misako U; Lemos, Francisco J A; Oliva, Maria Luiza

    2013-07-01

    As Aedes aegypti transmits the etiologic agents of both yellow and dengue fever; vector control is considered essential to minimise their incidence. The aim of this work was to identify the component of Carica papaya seed toxic to A. aegypti, and the identification of tegupain, the enzyme that generates it. Aqueous extracts (1%, w/v) of the seed tegument and cotyledon of C. papaya are not larvicidal isolately. However, a mixture of 17μgmL(-1) tegument extract and 27μgmL(-1) cotyledon extract caused 100% larval mortality in a bioassay. The mixture was no longer larvicidal after the tegument extract was pre-treated at 100°C for 10min. The enzyme tegupain efficiently hydrolysed the substrate Z-Phe-Arg-pNan (Km 58.8μM, Kcat 28020s(-1), Kcat/Km 5×10(8)M(-1) s(-1)), and its activity increased with 2mM dithiothreitol (DTT), at 37°C, pH 5.0. The chelating agent EDTA did not modify the enzyme activity. Inhibition of tegupain by cystatin (Kiapp 2.43nM), E64 (3.64nM, 83% inhibition), and the propeptide N-terminal sequence indicate that the toxic activity is due to a novel cysteine proteinase-like enzyme, rendered active upon the hydrolysis of a cotyledon component of C. papaya seeds. PMID:23402920

  2. Crystallization and preliminary X-ray analysis of a protease inhibitor from the latex of Carica papaya

    International Nuclear Information System (INIS)

    The Kunitz-type trypsin/chymotrypsin inhibitor isolated from C. papaya latex has been crystallized using the hanging-drop vapour-diffusion method. Two different crystal forms are observed, diffracting to 2.6 and 1.7 Å. A Kunitz-type protease inhibitor purified from the latex of green papaya (Carica papaya) fruits was crystallized in the presence and absence of divalent metal ions. Crystal form I, which is devoid of divalent cations, diffracts to a resolution of 2.6 Å and belongs to space group P31 or P32. This crystal form is a merohedral twin with two molecules in the asymmetric unit and unit-cell parameters a = b = 74.70, c = 78.97 Å. Crystal form II, which was grown in the presence of Co2+, diffracts to a resolution of 1.7 Å and belongs to space group P212121, with unit-cell parameters a = 44.26, b = 81.99, c = 140.89 Å

  3. Effect of Fitomas-E on seedling production of papaya var. Maradol Roja in Cienfuegos,Cuba

    Directory of Open Access Journals (Sweden)

    Fernando G. Serbelló Guzmán

    2016-02-01

    Full Text Available An experiment in nursery in bag conditions was carried out at the Cienfuegos Horticultural Company, in order to evaluate the effect of different alternatives to stimulate the growth and the development of papaya seedlings (Carica papaya L. variety Maradol Red, in the period from June to September 2010. The treatments were: applying Fitomas-E, Trichoderma, EcoMic, the combination of Trichoderma + EcoMic and a control without application. We evaluated the height and diameter at four different positions, the number of leaves, green and dry mass of roots as well as for the external part of the plant at the transplantation time. The obtained data were subjected to the given statistical analyzes. Results indicate that the height and diameter of the papaya plants are increased with the application of Fitomas E and EcoMic, while a higher number of leaves is reached with the application of Fitomas E, and the combination of EcoMic with Trichoderma.

  4. Efecto de la madurez, geometría y presión sobre la cinética de transferencia de masa en la deshidratación osmótica de papaya (Carica papaya L., var. Maradol

    Directory of Open Access Journals (Sweden)

    Lina Marcela Chavarro-Castrillón

    2006-09-01

    Full Text Available Las papayas obtenidas en el raleo (papayas menos desarrolladas entresacadas de los árboles para beneficiar el crecimiento de las otras generalmente se descartan. Alternativamente, estas papayas pueden secarse por un proceso de deshidratación osmótica y secado térmico convencional para usarlas como snacks o como ingrediente para otros productos. Se comparó la cinética de transferencia de masa en la deshidratación osmótica de papayas de raleo frente a papayas desarrolladas con diferente grado de madurez, considerando el efecto de la geometría de la muestra y de la presión del proceso. La deshidratación osmótica se efectuó en una solución agitada de sacarosa a 50 °Brix, a 25 °C. Se consideraron tres niveles de madurez: raleo, verde y pintona, tres geometrías: lámina, cilindro y anillo y dos niveles de presión: atmosférica y vacío. Se estudiaron como variables cinéticas la variación de peso (WR, pérdida de agua (WL y ganancia de sólidos (SG entre 10 y 180 min. La madurez tuvo efecto significativo sobre las tres respuestas de la cinética a 30 min y sobre SG a 180 min; la geometría tuvo efecto sobre WR y SG a 30 min y sobre los tres parámetros cinéticos a 180 min y la presión solamente tuvo efecto sobre WL y SG a 30 min. La mayor pérdida de agua (65% se obtuvo a 30 min para la combinación raleo/cilindro/vacío; mientras que la mayor ganancia de sólidos fue 31% para el tratamiento verde/lámina/vacío a 180 min.

  5. Artificial neural network analysis of genetic diversity in Carica papaya L. / Rede neural artificial na análise da divergência genética em Carica papaya L.

    Scientific Electronic Library Online (English)

    Cibelle Degel, Barbosa; Alexandre Pio, Viana; Silvana Silva Red, Quintal; Messias Gonzaga, Pereira.

    2011-09-01

    Full Text Available O estudo da diversidade genética é de fundamental importância na seleção preliminar de acessos com características superiores e a utilização desses materiais com sucesso em programas de melhoramento genético. O objetivo deste trabalho foi avaliar, como estratégia de análise da diversidade genética, [...] a técnica de bioinformática denominada rede neural artificial. Foi considerada a média de três épocas de plantio, oito caracteres quantitativos e trinta e sete acessos de mamoeiro, utilizando-se o delineamento em blocos casualizados com duas repetições. Com base na análise discriminante de Anderson, 91,90 % dos acessos foram classificados corretamente nos grupos previamente definidos pela rede neural artificial. Concluiu-se que a técnica de rede neural artificial se demonstrou viável na classificação dos acessos. Observou-se a presença significativa de diversidade genética entre os acessos avaliados. Abstract in english The study of genetic diversity is fundamental in the preliminary selection of accessions with superior characteristics and for a successful use of these genotypes in breeding programs. The purpose of this study was to evaluate, as a strategy for genetic diversity analysis, the bioinformatics approac [...] h called artificial neural network. Based on the average of three growing seasons, eight quantitative traits and thirty-seven papaya accessions were evaluated in a randomized complete block design, with two replications. By Anderson's discriminant analysis, 91.90 % of the accessions were correctly classified in the groups previously defined by artificial neural network. It was concluded that the technique of artificial neural network is feasible to classify the accessions. The presence of significant genetic diversity among accessions was observed.

  6. CADÁVER TUERTO, DE EDUARDO LABARCA EN EL MARCO DE LA "NOVELA HISTÓRICA CHILENA RECIENTE" Corpse, de Eduardo Labarca under The "Historical Novel Recent Chilean

    Directory of Open Access Journals (Sweden)

    Eduardo Barraza J

    2010-07-01

    Full Text Available Cadáver tuerto (2005 anticipa las variaciones del género novelesco en la literatura chilena, privilegiando y contemplando el proceso de la escritura. Labarca parodia en esta novela los cruces entre la ficción, la realidad y la historia, entre la vida y la autobiografía, entre el discurso televisivo, radial y del periodismo escrito, de modo tal que da un notorio giro discursivo a la narrativa chilena de filiación histórica.Cadáver tuerto (2005 anticipates the variations of the novel genre in Chilean literatura, priviledging and contemplating the writing process. Labarca parodies, in this novel, the crossings among fiction and reality, between life and autobiography, between television, radio and written newsprint discourse.

  7. Alimentación normal del niño menor de 2 años: Recomendaciones de la Rama de Nutrición de la Sociedad Chilena de Pediatría 2013

    Directory of Open Access Journals (Sweden)

    CARLOS CASTILLO-DURÁN

    2013-10-01

    Full Text Available Los modos de alimentación adquiridos por el lactante son centrales para su crecimiento y desarrollo, así como para la disminución del riesgo de diversas enfermedades crónicas; pueden alterar además las preferencias alimentarias en otras edades. La obesidad, las alergias y otras enfermedades crónicas asociadas a la nutrición han pasado a ser los problemas más prevalentes en los niños chilenos. Hay abundante evidencia científica reciente tanto internacional como nacional acerca de los modos de alimentación del lactante, que están haciendo indispensable una actualización de las guías chilenas de alimentación del año 2004. Este artículo actualiza y propone nuevas recomendaciones de alimentación para población chilena durante los primeros dos años de vida.

  8. 116 años de la Revista Chilena de Historia Natural: Breve relato de dos antes y un después / 116 years of Revista Chilena de Historia Natural: A brief story of two befores and an after

    Scientific Electronic Library Online (English)

    PATRICIO A, CAMUS.

    2013-12-01

    Full Text Available El año 2013 marcará el cierre de una etapa importante para la Revista Chilena de Historia Natural (RCHN), ya que a partir de 2014 comenzará a publicarse a través de la plataforma SpringerOpen, incluyendo algunos cambios relevantes en su diseño, manejo editorial, e idioma, entre otros. Por lo tanto e [...] n este artículo sintetizo algunos aspectos clave en la evolución de RCHN desde su fundación a fines del siglo 19, a través de dos períodos históricos: (a) 1897-1963, donde destaca la figura de su creador Carlos E. Porter y el rol de la Sociedad Chilena de Historia Natural, y (b) 1983-2013, su etapa reciente ligada a la Sociedad de Biología de Chile. Adicionalmente presento un breve análisis de la situación y el impacto de RCHN en su etapa moderna, incluyendo información inédita que muestra la preferencia (vía citación) por distintos aspectos de sus contenidos (categoría de artículo, área disciplinaria, tipo de ambiente), y un ranking de los artículos más citados vs. aquellos más consultados online, que revela dos caras distintas del impacto de la revista. Abstract in english The year 2013 will mark the end of an important period for Revista Chilena de Historia Natural (RCHN), as from 2014 it will start to be published through the SpringerOpen platform, including some conspicuous changes in design, editorial management and language, among others. Therefore in this articl [...] e I summarize some key aspects in the evolution of RCHN since its foundation in the late 19th century, through two historical periods: (a) 1897-1963, which highlights the figure of its creator Carlos E. Porter and the role of the Sociedad Chilena de Historia Natural (Chilean Society of Natural History), and (b) 1983-2013, its recent period linked to the Sociedad de Biología de Chile (Biology Society of Chile). Additionally, I present a brief analysis of the situation and the impact of RCHN in its modern period, including unpublished information showing the preference (via citation) for different aspects of its contents (article category, disciplinary area, environment type), and a ranking of the most cited articles vs. those most consulted online, which reveals two different faces of the journal's impact.

  9. Actividad antifúngica del aceite esencial de naranja (Citrus sinensis L.) sobre hongos postcosecha en frutos de lechosa (Carica papaya L.) / Anti-fungal activity of essential orange oil (Citrus sinensis L.) over post-harvest fungi in papaya fruits (Carica papaya L.)

    Scientific Electronic Library Online (English)

    Clemencia, Guédez; Luis, Cañizalez; Laura, Avendaño; José, Scorza; Carmen, Castillo; Rafael, Olivar; Yolanda, Méndez; Libert, Sánchez.

    2014-12-01

    Full Text Available Los hongos postcosecha causan pudriciones que afectan la calidad de los frutos de lechosa (Carica papaya L.). Actualmente los aceites esenciales son considerados una alternativa a los fungicidas químicos para controlar estos hongos. En este estudio se evaluó la actividad antifúngica del aceite esenc [...] ial de naranja (Citrus sinensis L.) sobre los hongos Colletotrichum gloeosporioides, Penicillium indicum, Fusarium solani, Rhizopus stolonifer y Aspergillus flavus, en medio de cultivo Papa Dextrosa Agar (PDA) en un diseño completamente al azar. Los resultados mostraron que existe un efecto inhibidor del aceite esencial de naranja (AEN) en el crecimiento micelial de los hongos in vitro, superior al 80% a concentración de AE de 1%, y 100% de inhibición a concentraciones de 2,5% y 5% de AE (p Abstract in english Post-harvest fungi produce rotting that affects the quality of papaya fruits (Carica papaya L.) At present, essential oils are considered an alternative to chemical anti-fungal substances for controlling these fungi. In this study we evaluated the antifungal activity of essential orange oil (Citrus [...] sinensis L.) over Colletotrichum gloeosporioides, Penicillum indicum, Fusarium solani, Rhizopus stolonifer and Aspergillus flavus, in Potato Dextrose Agar (PDA) culture medium in a completely random design. The results showed that there is an inhibitory effect of essential orange oil (EOO) over the in vitro mycelium growth of fungi, higher than 80% at a 1% EOO concentration and 100% inhibition at 2.5% and 5% EOO concentrations (p