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Sample records for hla-b27 transgenic rat

  1. HLA-B27-homodimer-specific antibody modulates the expansion of pro-inflammatory T-cells in HLA-B27 transgenic rats

    Osiris Marroquin Belaunzaran; Sascha Kleber; Stefan Schauer; Martin Hausmann; Flora Nicholls; Maries Van den Broek; Sravan Payeli; Adrian Ciurea; Simon Milling; Frank Stenner; Jackie Shaw; Simon Kollnberger; Paul Bowness; Ulf Petrausch; Christoph Renner

    2015-01-01

    Objectives: HLA-B27 is a common genetic risk factor for the development of Spondyloarthritides (SpA). HLA-B27 can misfold to form cell-surface heavy chain homodimers (B272) and induce pro-inflammatory responses that may lead to SpA pathogenesis. The presence of B272 can be detected on leukocytes of HLA-B27+ Ankylosing spondylitis (AS) patients and HLA-B27 transgenic rats. We characterized a novel B272–specific monoclonal antibody to study its therapeutic use in HLA-B27 associated disorder...

  2. Cytotoxic T-Cell-Mediated Response against Yersinia pseudotuberculosis in HLA-B27 Transgenic Rat

    Falgarone, Géraldine; Blanchard, Hervé S.; Riot, Bertrand; Simonet, Michel; Breban, Maxime

    1999-01-01

    Yersinia-induced reactive arthritis is highly associated with HLA-B27, the role of which in defense against the triggering bacteria remains unclear. The aim of this study was to examine the capacity of rats transgenic for HLA-B27 to mount a cytotoxic T-lymphocyte (CTL) response against Y. pseudotuberculosis and to determine the influence of the HLA-B27 transgene on this response. Rats transgenic for HLA-B*2705 and human β2-microglobulin of the 21-4L line, which do not spontaneously develop di...

  3. HLA-B27-Homodimer-Specific Antibody Modulates the Expansion of Pro-Inflammatory T-Cells in HLA-B27 Transgenic Rats.

    Osiris Marroquin Belaunzaran

    Full Text Available HLA-B27 is a common genetic risk factor for the development of Spondyloarthritides (SpA. HLA-B27 can misfold to form cell-surface heavy chain homodimers (B272 and induce pro-inflammatory responses that may lead to SpA pathogenesis. The presence of B272 can be detected on leukocytes of HLA-B27+ Ankylosing spondylitis (AS patients and HLA-B27 transgenic rats. We characterized a novel B272-specific monoclonal antibody to study its therapeutic use in HLA-B27 associated disorders.The monoclonal HD5 antibody was selected from a phage library to target cell-surface B272 homodimers and characterized for affinity, specificity and ligand binding. The immune modulating effect of HD5 was tested in HLA-B27 transgenic rats. Onset and progression of disease profiles were monitored during therapy. Cell-surface B272 and expansion of pro-inflammatory cells from blood, spleen and draining lymph nodes were assessed by flow cytometry.HD5 bound B272 with high specificity and affinity (Kd = 0.32 nM. HD5 blocked cell-surface interaction of B272 with immune regulatory receptors KIR3DL2, LILRB2 and Pirb. In addition, HD5 modulated the production of TNF from CD4+ T-cells by limiting B272 interactions in vitro. In an HLA-B27 transgenic rat model repetitive dosing of HD5 reduced the expansion of pro-inflammatory CD4+ T-cells, and decreased the levels of soluble TNF and number of cell-surface B272 molecules.HD5 predominantly inhibits early TNF production and expansion of pro-inflammatory CD4+ T-cells in HLA-B27 transgenic rats. Monoclonal antibodies targeting cell-surface B272 propose a new concept for the modulation of inflammatory responses in HLA-B27 related disorders.

  4. HLA-B27 and human β2-microglobulin affect the gut microbiota of transgenic rats.

    Phoebe Lin

    Full Text Available The HLA-B27 gene is a major risk factor for clinical diseases including ankylosing spondylitis, acute anterior uveitis, reactive arthritis, and psoriatic arthritis, but its mechanism of risk enhancement is not completely understood. The gut microbiome has recently been shown to influence several HLA-linked diseases. However, the role of HLA-B27 in shaping the gut microbiome has not been previously investigated. In this study, we characterize the differences in the gut microbiota mediated by the presence of the HLA-B27 gene. We identified differences in the cecal microbiota of Lewis rats transgenic for HLA-B27 and human β2-microglobulin (hβ2m, compared with wild-type Lewis rats, using biome representational in situ karyotyping (BRISK and 16S rRNA gene sequencing. 16S sequencing revealed significant differences between transgenic animals and wild type animals by principal coordinates analysis. Further analysis of the data set revealed an increase in Prevotella spp. and a decrease in Rikenellaceae relative abundance in the transgenic animals compared to the wild type animals. By BRISK analysis, species-specific differences included an increase in Bacteroides vulgatus abundance in HLA-B27/hβ2m and hβ2m compared to wild type rats. The finding that HLA-B27 is associated with altered cecal microbiota has not been shown before and can potentially provide a better understanding of the clinical diseases associated with this gene.

  5. Correlation of Cecal Microflora of HLA-B27 Transgenic Rats with Inflammatory Bowel Disease

    Onderdonk, Andrew B; Richardson, James A.; Hammer, Robert E.; Taurog, Joel D.

    1998-01-01

    Transgenic rats with a high level of expression of the human major histocompatibility complex class I molecule HLA-B27 develop chronic inflammatory bowel disease (IBD) and arthritis. Assessment of the cecal microflora showed a rise in numbers of Escherichia coli and Enterococcus spp., corresponding to the presence and severity of IBD in these rats.

  6. Lactobacillus GG prevents recurrence of colitis in HLA-B27 transgenic rats after antibiotic treatment

    Dieleman, L A; Goerres, M S; Arends, A.; Sprengers, D; Torrice, C; Hoentjen, F; Grenther, W B; Sartor, R. B.

    2003-01-01

    Background and aims: Bacteroides vulgatus induces colitis in gnotobiotic HLA-B27 transgenic (TG) rats while broad spectrum antibiotics prevent and treat colitis in specific pathogen free (SPF) TG rats although disease recurs after treatment ends. Lactobacilli treat human pouchitis and experimental colitis. We investigated if Lactobacillus rhamnosus GG (L GG) can prevent colitis in TG rats monoassociated with B vulgatus and if L GG or Lactobacillus plantarum 299v (LP 299v) can treat establishe...

  7. Transfer of the inflammatory disease of HLA-B27 transgenic rats by bone marrow engraftment

    1993-01-01

    We have previously produced lines of rats transgenic for HLA-B27 and human beta 2-microglobulin (h beta 2m) that develop a progressive inflammatory disease sharing many clinical and histologic features with the B27-associated human spondyloarthropathies, including gut and male genital inflammation, arthritis, and psoriasiform skin lesions. Other transgenic lines that express lower levels of B27 and h beta 2m remain healthy. To investigate the cellular basis for the multisystem inflammatory di...

  8. A monoclonal antibody against kininogen reduces inflammation in the HLA-B27 transgenic rat

    Keith, James C.; Sainz, Irma M.; Isordia-Salas, Irma; Pixley, Robin A.; Leathurby, Yelena; Albert, Leo M.; Colman, Robert W.

    2005-01-01

    The human leukocyte antigen B27 (HLA-B27) transgenic rat is a model of human inflammatory bowel disease, rheumatoid arthritis and psoriasis. Studies of chronic inflammation in other rat models have demonstrated activation of the kallikrein–kinin system as well as modulation by a plasma kallikrein inhibitor initiated before the onset of clinicopathologic changes or a deficiency in high-molecular-mass kininogen. Here we study the effects of monoclonal antibody C11C1, an antibody against high-mo...

  9. HLA-B27 and Human β2-Microglobulin Affect the Gut Microbiota of Transgenic Rats

    Phoebe Lin; Mary Bach; Mark Asquith; Lee, Aaron Y.; Lakshmi Akileswaran; Patrick Stauffer; Sean Davin; Yuzhen Pan; Cambronne, Eric D.; Martha Dorris; Debelius, Justine W.; Lauber, Christian L.; Gail Ackermann; Baeza, Yoshiki V.; Tejpal Gill

    2014-01-01

    The HLA-B27 gene is a major risk factor for clinical diseases including ankylosing spondylitis, acute anterior uveitis, reactive arthritis, and psoriatic arthritis, but its mechanism of risk enhancement is not completely understood. The gut microbiome has recently been shown to influence several HLA-linked diseases. However, the role of HLA-B27 in shaping the gut microbiome has not been previously investigated. In this study, we characterize the differences in the gut microbiota mediated by t...

  10. Supplemental Antioxidants Do Not Ameliorate Colitis Development in HLA-B27 Transgenic Rats Despite Extremely Low Glutathione Levels in Colonic Mucosa

    Schepens, M.A.A.; Vink, C.; Schonewille, A.J.; Roelofs, H.M.J.; Brummer, R.J.; Meer, van der R.; Bovee-Oudenhoven, I.M.J.

    2011-01-01

    Background: Oxidative stress is presumed to play an important role in inflammatory bowel disease (IBD). Accordingly, antioxidant supplementation might be protective. Dietary calcium inhibited colitis development in HLA-B27 transgenic rats, an animal model mimicking IBD. As antioxidants might act at

  11. Normal luminal bacteria, especially Bacteroides species, mediate chronic colitis, gastritis, and arthritis in HLA-B27/human beta2 microglobulin transgenic rats.

    Rath, H C; Herfarth, H H; Ikeda, J S; Grenther, W B; Hamm, T E; Balish, E; Taurog, J D; Hammer, R. E.; Wilson, K H; Sartor, R B

    1996-01-01

    Genetic and environmental factors are important in the pathogenesis of clinical and experimental chronic intestinal inflammation. We investigated the influence of normal luminal bacteria and several groups of selected bacterial strains on spontaneous gastrointestinal and systemic inflammation in HLA-B27 transgenic rats. Rats maintained germfree for 3-9 mo were compared with littermates conventionalized with specific pathogen-free bacteria. Subsequently, germfree transgenic rats were colonized...

  12. Spontaneous inflammatory arthritis in HLA-B27 transgenic mice lacking beta 2-microglobulin: a model of human spondyloarthropathies

    1995-01-01

    Human class I major histocompatibility complex allele HLA-B27 is associated with a group of human diseases called "spondyloarthropathies." Studies on transgenic rats expressing HLA-B27 and human beta 2-microglobulin have confirmed the role of HLA-B27 in disease pathogenesis. Here we report spontaneous inflammatory arthritis in HLA-B27 transgenic mice lacking beta 2-microglobulin (B27+ beta 2m-/- ). In the absence of beta 2-microglobulin, B27+ beta 2m-/- animals do not express the HLA-B27 tran...

  13. Dietary calcium inhibits colitis development in HLA-B27 transgenic rats [Rattus norvegicus

    Schothorst, van E.M.; Duivenvoorde, L.P.M.

    2009-01-01

    (Submitter supplied) Background and Aims: We have shown in several controlled rat and human infection studies that dietary calcium improves intestinal resistance and strengthens the mucosal barrier. Reinforcement of gut barrier function is also relevant for inflammatory bowel disease (IBD). Therefor

  14. Chemically defined diet alters the protective properties of fructo-oligosaccharides and isomalto-oligosaccharides in HLA-B27 transgenic rats.

    Petya Koleva

    Full Text Available Non-digestible oligosaccharides (NDO were shown to reduce inflammation in experimental colitis, but it remains unclear whether microbiota changes mediate their colitis-modulating effects. This study assessed intestinal microbiota and intestinal inflammation after feeding chemically defined AIN-76A or rat chow diets, with or without supplementation with 8 g/kg body weight of fructo-oligosaccharides (FOS or isomalto-oligosaccharides (IMO. The study used HLA-B27 transgenic rats, a validated model of inflammatory bowel disease (IBD, in a factorial design with 6 treatment groups. Intestinal inflammation and intestinal microbiota were analysed after 12 weeks of treatment. FOS and IMO reduced colitis in animals fed rat chow, but exhibited no anti-inflammatory effect when added to AIN-76A diets. Both NDO induced specific but divergent microbiota changes. Bifidobacteria and Enterobacteriaceae were stimulated by FOS, whereas copy numbers of Clostridium cluster IV were decreased. In addition, higher concentrations of total short-chain fatty acids (SCFA were observed in cecal contents of rats on rat chow compared to the chemically defined diet. AIN-76A increased the relative proportions of propionate, iso-butyrate, valerate and iso-valerate irrespective of the oligosaccharide treatment. The SCFA composition, particularly the relative concentration of iso-butyrate, valerate and iso-valerate, was associated (P ≤ 0.004 and r ≥ 0.4 with increased colitis and IL-1 β concentration of the cecal mucosa. This study demonstrated that the protective effects of fibres on colitis development depend on the diet. Although diets modified specific cecal microbiota, our study indicates that these changes were not associated with colitis reduction. Intestinal inflammation was positively correlated to protein fermentation and negatively correlated with carbohydrate fermentation in the large intestine.

  15. Expression of HLA-B27 in transgenic mice is dependent on the mouse H-2D genes

    1990-01-01

    HLA-B27 transgenic mice in the context of various H-2 haplotypes were produced. A high expression of the HLA-B27 antigen was observed in mice homozygous for H-2b, H-2f, H-2s, H-2p, H-2r, and H-2k haplotypes. Mice of the H-2v haplotype expressed HLA-B27 at an intermediate level. Expression of HLA-B27 was minimal in mice of the H-2q and H-2d haplotypes. This was observed both on the B10 background and in DBA/2 or BALB/c mice. Only minimal expression of HLA-B27 could be detected in B10.PL (KuDd)...

  16. Supplemental calcium attenuates the colitis-related increase in diarrhea, intestinal permeability, and extracellular matrix breakdown in HLA-B27 transgenic rats.

    Schepens, M.A.; Schonewille, A.J.; Vink, C.; Schothorst, E.M. van; Kramer, E.; Hendriks, T.; Brummer, R.J.; Keijer, J.; Meer, R. van der; Bovee-Oudenhoven, I.M.

    2009-01-01

    We have shown in several controlled rat and human infection studies that dietary calcium improves intestinal resistance and strengthens the mucosal barrier. Reinforcement of gut barrier function may alleviate inflammatory bowel disease (IBD). Therefore, we investigated the effect of supplemental cal

  17. Rat MHC-linked peptide transporter alleles strongly influence peptide binding by HLA-B27 but not B27-associated inflammatory disease

    Simmons, W.A.; Satumtira, Nimman; Taurog, J.D. [Univ. of Texas Southwestern Medical Center, Dallas, TX (United States)] [and others

    1996-02-15

    Rats transgenic for the human MHC molecule HLA-B27 were used to study the effect of two alleles, cim{sup a} and cim{sup b}, which are associated with peptide transport by the MHC-encoded Tap2 transporter, on the function of HLA-B27 as a restriction element for CTL recognition of the male H-Y minor H Ag and on the multisystem inflammatory disease characteristic of B27 transgenic rats. Anti-H-Y CTL generated in cim{sup a} B27 transgenic rats lysed male B27 cim{sup b/b} targets significantly less well than cim{sup a/a} or cim{sup a/b} targets. Addition of exogenous H-Y peptides to male B27 cim{sup b/b} targets increased susceptibility to lysis to the level of cim{sup a/a} targets sensitized with exogenous H-Y peptides. {sup 3}H-labeled peptides eluted from B27 molecules of lymphoblasts from rats of two cim{sup b} and three cim{sup a} RT1 haplotypes showed that the cim{sup b} peptide pool favors comparatively longer and/or more hydrophobic peptides. These results indicate that RT1-linked Tap2 polymorphism in the rat strongly influences peptide loading of HLA-B27. Nonetheless, the prevalence and severity of multisystem inflammatory lesions were comparable in backcross rats bearing either cim{sup a/b} or cim{sup b/b}. It thus appears either that binding of specific peptides to B27 is unimportant in the pathogenesis of B27-associated disease or that the critical peptides, unlike H-Y and many others, are not influenced by Tap transporter polymorphism. 42 refs., 6 figs., 3 tabs.

  18. Lack of expression of HLA-B27 gene in transgenic mouse trophoblast. Conserved genetic pressures underlying extra-embryonic development

    1989-01-01

    The mechanisms that regulate developmental control of the expression of MHC class I genes during generation of extra-embryonic tissues are largely unknown. In the present study, we studied the levels of transcripts of the human HLA-B27 gene in extra-embryonic tissues of transgenic mice containing the HLA-B27 (heavy chain) gene by in situ hybridization with biotinylated single-stranded RNA probes. In contrast to extra-embryonic stromal cells and embryonic tissues which contain (varying levels ...

  19. HLA-B27 heavy chains contribute to spontaneous inflammatory disease in B27/human beta2-microglobulin (beta2m) double transgenic mice with disrupted mouse beta2m.

    Khare, S D; Hansen, J.; Luthra, H S; David, C S

    1996-01-01

    MHC class I allele, HLA-B27, is strongly associated with a group of human diseases called spondyloarthropathies. Some of these diseases have an onset after an enteric or genitourinary infection. In the present study, we describe spontaneous disease in HLA-B27 transgenic mice where endogenous beta2-microglobulin (beta2m) gene was replaced with transgenic human beta2m gene. These mice showed cell surface expression of HLA-B27 similar to that of human peripheral blood mononuclear cells. In addit...

  20. HLA-B27 antigen

    ... colitis Psoriatic arthritis (arthritis associated with psoriasis) Reactive arthritis Sacroiliitis (inflammation of the sacroiliac joint) Uveitis If there are symptoms or signs of an autoimmune disease, a positive HLA-B27 test may confirm the diagnosis. However, HLA-B27 ...

  1. Complement activation and HLA-B27.

    Meri, S.; Partanen, J; Leirisalo-Repo, M; Repo, H

    1988-01-01

    The efficiency of complement activation was studied in sera from HLA-B27 positive and negative subjects (27 with previous yersinia arthritis and 35 controls). Activation of complement with zymosan induced higher mean levels of the anaphylatoxin C3a in HLA-B27 positive sera (mean (SD) 7.40 (1.66) mg/l) than in HLA-B27 negative sera (6.41 (1.79) mg/l). Similarly, higher levels of C3d,g, another C3 breakdown fragment, were obtained in HLA-B27 positive sera after Escherichia coli 0111:B4 lipopoly...

  2. HLA-B27相关前葡萄膜炎的研究进展%Advancement in the investigation of HLA-B27 associated anterior uveitis

    胡小凤; 陈巍; 卢弘

    2009-01-01

    Uveitis is an important autoimmune disease.Human leukocyte antigen (HLA)-B27-associated anterior uveitis is the most common form of anterior uveitis.HLA-B27-associated anterior uveitis has distinct ocular,systemic,and genetic features.It attacks young subjects and leads to the damage of visual function due to the recurrence and complications.However,the pathologic mechanism of HLA-B27-associated anterior uveitis is still not thoroughly understood,and effective therapy is still unavailable.There are currently many experimental researches which attamp to study the etiology and develop an effective therapy for HLA-B27 acute anterior uveitis.Thus,various animal models of acute anterior uveitis such as endotoxin-induced uveitis and HLA-B27 transgenic animals including rat and mouse are established.Different therapy regimens have been designed both in clinical trial and experimental study and aquired promising achievements.Epidemiology,pathologic mechanism,different animal models and potential new therapies such as anti-TNFα,oral associated peptides tolerance and gene therapy are overviewed.%HLA-B27阳性前葡萄膜炎是前葡萄膜炎最常见的类型,患者多为青壮年,反复发作,常引起一些并发症,严重影响视力,并可伴有其他系统性疾病.但其发病机制目前仍不清楚,尚无特效的治疗方法.近年来,建立了一些前葡萄膜炎动物模型,如内毒素诱导的前葡萄膜炎和HLA-B27转基因动物模型,并进行了一系列的研究.一些新的治疗方法在临床及动物实验中也取得了很好的疗效.就HLA-B27相关前葡萄膜炎的发病机制、流行病学、动物模型及一些新的治疗方法进行综述.

  3. Seronegative pauciarticular arthritis and HLA B27.

    Eastmond, C J; Rajah, S M; D. Tovey; Wright, V.

    1980-01-01

    Twenty-six patients with a pauciarticular arthritis have been studied clinically, radiologically and with histocompatibility typing. An increased frequency of HLA B27 was found (p = 1.87 x 10(-12)). Low back and buttock pain, Achilles tendinitis and dactylitis of the toes were more frequent in HLA-B27 positive patients. It is suggested that histocompatibility testing may be of some value in diagnosis and in the investigation of the possible 'reactive' nature of this type of arthritis.

  4. Polymorphism of HLA-B27: 105 subtypes currently known.

    Khan, Muhammad Asim

    2013-10-01

    HLA-B27 has a high degree of genetic polymorphism, with 105 known subtypes, named HLA-B*27:01 to HLA-B*27:106, encoded by 132 alleles. The most common subtypes associated with ankylosing spondylitis are HLA-B*27:05 (Caucasians), HLA-B*27:04 (Chinese), and HLA-B*27:02 (Mediterranean populations). For Chinese populations, HLA-B*27:04 is associated with a greater ankylosing spondylitis risk than HLA-B*27:05. Two subtypes, HLA-B27*06 and HLA-B27*09, seem to have no disease association. These differential disease associations of HLA-B27 subtypes, and the recent discovery that ERAP1 is associated with ankylosing spondylitis for patients with HLA-B27, have increased attempts to determine the function of HLA-B27 in disease pathogenesis by studying hemodynamic features of its protein structure, alterations of its peptidome, aberrant peptide handling, and associated molecular events. However, after 40 years we still do not fully know how HLA-B27 predisposes to ankylosing spondylitis and related spondyloarthritis. PMID:23990399

  5. Heart conduction disturbance: an HLA-B27 associated disease.

    Peeters, A J; ten Wolde, S; Sedney, M.I.; de Vries, R R; Dijkmans, B A

    1991-01-01

    In recent studies from Sweden an increased prevalence of HLA-B27 associated diseases and of HLA-B27 was found in an unselected group of men with permanently implanted pacemakers and with a heart block. Furthermore, a significantly increased prevalence of HLA-B27 was found in men with a pacemaker who had no clinical or radiological signs of HLA-B27 associated disease. To obtain more insight into the association between HLA-B27 and heart block, and the possible role of HLA-B27 in causing this b...

  6. HLA-B27 subtypes among the Chukotka native groups

    Krylov, M.Y.; Alexeeva, L.I.; Erdesz, S.; Benevolenskaya, L.I. [Akademiya Meditsinskikh Nauk SSSR, Moscow (Russian Federation). Inst. Revmatizma; Reveille, J.D.; Arnett, F.C. [Texas Univ., Houston, TX (United States). Health Science Center

    1995-12-31

    The purpose of this study was to assess the relative frequency of the known HLA-B27 subtypes in HLA-B27 positive Chukotka natives, which have higher frequencies of HLA-B27 (to 40%) and spondylarthropathies (to 2%) than the Russian Caucasian population. Using oligotyping of the polymerase-chain reaction amplified second and third exons of the HLA-B27 gene in 86 DNA samples from HLA-B27 positive individuals were successfully typed. All had HLA-B*2705, including 4 patients with Reiter`s syndrome and 5 with ankylosing spondyloarthritis, except one Eskimo who had HLA-B*2702. None had HLA-B*2704, a frequent subtype in Orientals. With respect to HLA-B27 subtypes the indigenous populations from the eastern part of the Chukotka Peninsula are genetically more closely related to Caucasians than to Orientals. (author). 18 refs, 1 fig., 2 tabs.

  7. HLA-B27 Misfolding and Ankylosing Spondylitis

    Colbert, Robert A.; Tran, Tri M.; Layh-Schmitt, Gerlinde

    2013-01-01

    Understanding how HLA-B27 contributes to the pathogenesis of spondyloarthritis continues to be an important goal. Current efforts are aimed largely on three areas of investigation; peptide presentation to CD8 T cells, abnormal forms of the HLA-B27 heavy chain and their recognition by leukocyte immunoglobulin-like receptors on immune effector cells, and HLA-B27 heavy chain misfolding and intrinsic biological effects on affected cells. In this chapter we review our current understanding of the ...

  8. HLA-B27 Positivity: associated health implications

    Cox, C. L.; Gibbons, H.; Evans, P; Withers, T.; Titmus, K.

    2011-01-01

    HLA-B27 positivity makes the onset of autoimmune diseases such as uveitis, ankylosing spondylitis and Crohn's disease more likely to occur. Ankylosing spondylitis and Crohn's disease are two types of HLA-B27 positive diseases that demonstrate a direct association with uveitis. Although the possession of HLA-B27 positivity is not mandatory for autoimmune diseases such as uveitis to occur, HLA-B27 positivity not only makes it more likely but may modify the clinical picture in which a patient pr...

  9. HLA-B27 typing in the categorisation of uveitis in a HLA-B27 rich population

    Huhtinen, M; Karma, A

    2000-01-01

    AIMS—To determine whether HLA-B27 typing helps the clinician in the diagnostic examination of uveitis in a HLA-B27 rich population and also whether the clinical picture of HLA-B27 positive unilateral acute or recurrent anterior uveitis (AAU) is distinguishable from the idiopathic negative form.
METHODS—During a 3 year period 220 consecutive patients with undetermined uveitis at onset were examined in the Helsinki University Eye Clinic. HLA-B27 antigen was tested for 85% of the patients. Other...

  10. Isolated HLA-B27 associated Achilles tendinitis.

    I. Olivieri; Gemignani, G; Gherardi, S; Grassi, L.; M.L. Ciompi

    1987-01-01

    The case of a 37 year old man with a longstanding HLA-B27 associated bilateral Achilles tendinitis without seronegative spondyloarthropathy is reported. This case suggests that heel enthesopathy may for a long time be the only clinical manifestation of the HLA-B27 associated disease process.

  11. Autoinflammation and HLA-B27: Beyond Antigen Presentation.

    Sibley, Cailin H

    2016-08-01

    HLA-B27 associated disorders comprise a group of inflammatory conditions which have in common an association with the HLA class I molecule, HLA-B27. Given this association, these diseases are classically considered disorders of adaptive immunity. However, mounting data are challenging this assumption and confirming that innate immunity plays a more prominent role in pathogenesis than previously suspected. In this review, the concept of autoinflammation is discussed and evidence is presented from human and animal models to support a key role for innate immunity in HLA-B27 associated disorders. PMID:27229619

  12. Bartonella henselae associated uveitis and HLA-B27

    Kerkhoff, F.T.; Rothova, A

    2000-01-01

    AIM—To investigate the frequency of HLA-B27 in patients with presumed Bartonella henselae associated uveitis and to describe the clinical characteristics of HLA-B27 positive patients with uveitis and presumed ocular bartonellosis (POB).
METHODS—The diagnosis of POB was considered in 19 patients with unexplained uveitis (except for the HLA-B27 association) and high positive IgG (titre ⩾1:900) and/or IgM (titre ⩾1:250) antibodies against B henselae. In addition to B henselae serology and HLA-B2...

  13. The role of HLA-B27 in inflammatory arthritis

    Lynch, Sarah Janice

    2009-01-01

    Electronic version excludes material for which permission has not been granted by the rights holder The MHC class I allele, HLA-B27, is strongly associated with a group of inflammatory arthritic conditions collectively known as spondyloarthropathies (SpA). Ankylosing spondylitis (AS) shows the strongest association with 90-95 % of patients being HLA-B27 positive. The relationship between HLA-B27 and SpA has been known for over 30 years, however despite ongoing research, the reason for thi...

  14. Enhanced neutrophil migration in vivo HLA B27 positive subjects.

    Koivuranta-Vaara, P; Repo, H; Leirisalo, M; Kiistala, U; Osterman, T; Vapaatalo, H

    1984-01-01

    Chemotaxis of polymorphonuclear leucocytes in vivo was studied in patients with previous yersinia arthritis and in healthy subjects with or without HLA B27 by means of a skin chamber technique. Irrespective of previous arthritis the number of neutrophils in the chamber media was significantly higher in HLA B27 positive subjects than in those without HLA B27. The amounts of prostaglandins E2, F2 alpha, and 6-keto-F1 alpha in the chamber media correlated positively with the corresponding cell c...

  15. HLA-B27和强直性脊柱炎%HLA-B27 antigen and ankylosing spondylitis

    陶赞英; 郑明慈

    2006-01-01

    HLA-B27与强直性脊柱炎呈强相关,但它在强直性脊柱炎中作用仍不详.近年来人群中HLA-B27基因亚型的研究和人类B27转基因动物模型的建立都有力地证实了HLA-B27分子是强直性脊柱炎原发关联成分.

  16. Multiple joint tuberculosis presenting as HLA-B27 disease

    Hopkins, G. O.

    1983-01-01

    A case of multifocal osteoarticular tuberculosis in a young Caucasian male is presented. The diagnostic difficulty, compounded by slow progression of the disorder and the presence of the tissue antigen HLA-B27, is discussed.

  17. Coexisting HLA-B27 positive spondyloarthritis and polyarteritis nodosa.

    Sattar, M A

    1992-01-01

    A 38 year old woman presented with widespread polyarteritis nodosa a few years after the onset of HLA-B27 positive spondyloarthritis. The concomitant coexistence of these two disorders suggests a possible association in this genetically susceptible subject.

  18. Pathogenicity of Misfolded and Dimeric HLA-B27 Molecules

    Antoniou, Antony N.; Izabela Lenart; Guiliano, David B.

    2011-01-01

    The association between HLA-B27 and the group of autoimmune inflammatory arthritic diseases, the spondyloarthropathies (SpAs) which include ankylosing spondylitis (AS) and Reactive Arthritis (ReA), has been well established and remains the strongest association between any HLA molecule and autoimmune disease. The mechanism behind this striking association remains elusive; however animal model and biochemical data suggest that HLA-B27 misfolding may be key to understanding its association with...

  19. Invasion and persistence of Salmonella in human fibroblasts positive or negative for endogenous HLA B27

    Huppertz, H; Heesemann, J

    1997-01-01

    OBJECTIVE—Analysis of the interaction of enteropathogenic bacteria with HLA B27 transfected murine fibroblasts showed a specific influence of HLA B27 on microbial invasiveness. This possible novel mechanism for the action of HLA B27 should be verified by using endogenous HLA B27 positive and negative human fibroblasts as a model for the direct interaction of arthritogenic bacteria and host cells.
METHODS—Fibroblasts were obtained from healthy donors positive or negative for HLA B27; cultivate...

  20. Pathogenicity of Misfolded and Dimeric HLA-B27 Molecules

    Antony N. Antoniou

    2011-01-01

    Full Text Available The association between HLA-B27 and the group of autoimmune inflammatory arthritic diseases, the spondyloarthropathies (SpAs which include ankylosing spondylitis (AS and Reactive Arthritis (ReA, has been well established and remains the strongest association between any HLA molecule and autoimmune disease. The mechanism behind this striking association remains elusive; however animal model and biochemical data suggest that HLA-B27 misfolding may be key to understanding its association with the SpAs. Recent investigations have focused on the unusual biochemical structures of HLA-B27 and their potential role in SpA pathogenesis. Here we discuss how these unusual biochemical structures may participate in cellular events leading to chronic inflammation and thus disease progression.

  1. Reduction of colitis by prebiotics in HLA-1327 transgenic rats is associated with microflora changes and immunomodulation

    Hoentjen, F; Welling, GW; Harmsen, HJM; Zhang, XY; Snart, J; Tannock, GW; Lien, K; Churchill, TA; Lupicki, M; Dieleman, LA

    2005-01-01

    HLA-B27 transgenic rats develop spontaneous colitis under specific pathogen-free conditions (SPF) but germ-free rats remain disease-free, emphasizing a role for intestinal bacteria in the pathogenesis of chronic intestinal inflammation. Prebiotics are dietary substances that affect the host by stimu

  2. HLA-B27, but not HLA-B7, immunodominance to influenza is ERAP dependent.

    Akram, Ali; Lin, Aifeng; Gracey, Eric; Streutker, Catherine J; Inman, Robert D

    2014-06-15

    Endoplasmic reticulum-associated aminopeptidase-1 (ERAP1) plays a critical role in the processing of peptides prior to binding to MHC class I molecules. In this article, we show for the first time, to our knowledge, that the HLA-B27 immunodominant influenza nucleoprotein (NP) 383-391 epitope is made as an N-terminally extended 14-mer before it is trimmed by ERAP. In the absence of ERAP, there is a significant reduction in the CTL response to the B27/NP383-391 epitope in influenza A (flu)-infected B27/ERAP(-/-) mice. With the use of tetramer staining, the number of naive CD8(+) T cells expressing TCR Vβ8.1 in B27/ERAP(-/-) transgenic mice is significantly lower than that seen in B27/ERAP(+/+) mice. HLA-B27 surface expression in naive and flu-infected B27/ERAP(-/-) mice is also lower than the expression seen for the same allele in naive and flu-infected B27/ERAP(+/+) mice. In contrast, surface expression of HLA-B7 was unaffected by the absence of ERAP in B7/ERAP(-/-) transgenic mice. The B7-restricted NP418-426 CTL response in flu-infected B7/ERAP(-/-) and B7/ERAP(+/+) mice was also similar. These results provide, to our knowledge, the first in vivo demonstration of ERAP functionally influencing host immune response in an HLA allele-specific manner. This principle has relevance to diseases such as ankylosing spondylitis, in which HLA-B27 and ERAP jointly contribute to disease predisposition. PMID:24835397

  3. Aortic incompetence in HLA B27-positive juvenile arthritis.

    Kean, W F; Anastassiades, T. P.; Ford, P M

    1980-01-01

    The early onset of isolated aortic incompetence in a male child with HLA B27 and peripheral arthritis is reported. Acute anterior uveitis and lone aortic incompetence occurred at 1 and 9 months respectively after the development of the acute inflammatory arthritis. The uveitis resolved with local therapy and the arthritis remitted 10 months after the onset. There has been no recurrence of the arthritis after 10 years of close follow-up but the aortic incompetence has persisted, though it rema...

  4. Acute anterior uveitis, ankylosing spondylitis, back pain, and HLA-B27.

    Beckingsale, A. B.; Davies, J.; Gibson, J M; Rosenthal, A R

    1984-01-01

    One hundred and sixty-nine patients with acute anterior uveitis were studied for the presence of HLA-B27 tissue type, radiological evidence of ankylosing spondylitis, and a history of back pain. 60% were male; 45% were HLA-B27+. The male:female ratio in the HLA-B27+ group was the same as in the whole group. 24% had radiological evidence of ankylosing spondylitis, and, of these, 83% were HLA-B27+ while 17% were HLA-B27-. There was a definite correlation between the severity of the ankylosing s...

  5. HLA-B*27 typing by sequence specific amplification without DNA extraction.

    Sayer, D. C.; Cassell, H S; Christiansen, F. T.

    1999-01-01

    HLA-B27 appears to play a direct role in the pathogenesis of ankylosing spondylitis and almost all patients with this disease have HLA-B27. Therefore, a diagnosis of ankylosing spondylitis can virtually be excluded in the absence of HLA-B27. Many techniques have been used for HLA-B*27 typing. Of these, molecular methods are the most sensitive and specific but require extracted DNA as the testing material. A technique where HLA-B*27 is amplified directly from whole blood using sequence specifi...

  6. Prevalence of HLA-B27 in the New Zealand population: effect of age and ethnicity

    Roberts, Rebecca L; Wallace, Mary C.; Jones, Gregory T.; van Rij, Andre M.; Tony R Merriman; Harrison, Andrew; White, Douglas; Stamp, Lisa K.; Ching, Daniel; Highton, John; Stebbings, Simon M

    2013-01-01

    Introduction HLA-B27 genotyping is commonly used to support a diagnosis of ankylosing spondylitis (AS). A recent study has suggested that HLA-B27 may adversely affect longevity. The objectives of this study were to determine, for the first time, the prevalence of HLA-B27 in the New Zealand population, and to test whether HLA-B27 prevalence declines with age. Methods 117 Caucasian controls, 111 New Zealand Māori controls, and 176 AS patients were directly genotyped for HLA-B27 using PCR-SSP. T...

  7. HLA-B27 associated spondyloarthropathy, an autoimmune disease based on crossreactivity between bacteria and HLA-B27?

    Ringrose, J.H.

    1999-01-01

    Most autoimmune diseases are associated with certain HLA types. Therefore, spondyloarthropathies (SpA) strongly associated with HLA-B27, are also often classified as autoimmune diseases. This study questions whether SpA indeed fulfils the criteria of an autoimmune disease. The Medline database was searched for all reports between 1966 and April 1998 on the presence of autoimmune reactivity in SpA patients. This search yielded 45 articles on this subject. Only eight articles study T cell react...

  8. Seasonal variation of acute anterior uveitis: differences between HLA-B27 positive and HLA-B27 negative disease.

    Ebringer, R; White, L.; McCoy, R; Tait, B

    1985-01-01

    One hundred and seventy-five consecutive patients with acute anterior uveitis (AAU) were examined over a 24-month period. There was a significantly increased incidence of AAU during the months August to December (p less than 0.05). This increase was confined predominantly to the HLA-B27 negative group of patients (p less than 0.01). There was no significant monthly difference in incidence between males and females, between patients with first or recurrent attacks, or between patients with and...

  9. HLA-B27 Anterior Uveitis: Immunology and Immunopathology.

    Wakefield, Denis; Yates, William; Amjadi, Shahriar; McCluskey, Peter

    2016-08-01

    Acute anterior uveitis (AAU) is the commonest type of uveitis and HLA-B27 AAU is the most frequently recognized type of acute anterior uveitis and anterior uveitis overall. Recent evidence indicates that acute anterior uveitis is a heterogenous disease, is polygenic and is frequently associated with the spondyloarthropathies (SpA). Studies of patients with AAU and animal models of disease indicate a role for innate immunity, the IL-23 cytokine pathway and exogenous factors, in the pathogenesis of both SpA and acute anterior uveitis. Recently described genetic associations cluster around immunologic pathways, including the IL-17 and IL-23 pathways, antigen processing and presentation, and lymphocyte development and activation. Patients with ankylosing spondylitis (AS) and AAU share other genetic markers, such as ERAP-1, which show strong evidence of gene-gene interaction and point to new mechanisms of disease pathogenesis. These observations have major implications for understanding the pathogenesis of HLA-B27 diseases, such as AAU, and may lead to the development of more specific therapy for AAU. Received 6 January 2016; revised 6 February 2016; accepted 18 February 2016; published online 31 May 2016. PMID:27245590

  10. HLA-B27: natural function and pathogenic role in spondyloarthritis

    McMichael, Andrew; Bowness, Paul

    2002-01-01

    Chapter summary The human leukocyte antigen HLA-B27 is strongly associated with development of a group of inflammatory arthritides collectively known as the spondyloarthritides. We have set out to define the natural immunological function of HLA-B27, and then to apply this knowledge to understand its pathogenic role. Human leukocyte antigen class 1 molecules bind antigenic peptides for cell surface presentation to cytotoxic T lymphocytes. HLA-B27 binds and presents peptides from influenza, HI...

  11. HLA B27 allele types in homogeneous groups of juvenile idiopathic arthritis patients in Latvia

    Guseinova Dinara; Lazareva Arina; Sochnevs Arturs; Zavadska Dace; Eglite Jelena; Stanevicha Valda; Shantere Ruta; Gardovska Dace

    2010-01-01

    Abstract Juvenile idiopathic arthritis (JIA) is a heterogeneous condition and therapeutic strategies vary in different JIA types. The routinely accepted practice to start with Sulphasalazine (SS) as the first line treatment in patients with HLA B27 positive JIA proves to be ineffective in a large proportion of children. Objective to investigate HLA B27 positive JIA patients clinical characteristics, determined HLA B27 allele types and their connection with antirheumatic treatment in homogenou...

  12. HLA-B27 frequency in a group of patients with psoriatic arthritis *

    Danilo Garcia Ruiz; Mário Newton Leitão de Azevedo; Omar Lupi

    2012-01-01

    BACKGROUND: HLA-B27 is associated with spondyloarthritis, a group of diseases that includes psoriatic arthritis. OBJECTIVES: To describe the HLA-B27 frequency in a group of Brazilian patients with psoriatic arthritis and correlate its presence or absence with their clinical manifestations. METHODS: Cross-sectional study with 44 psoriatic arthritis patients of a Rheumatology clinic. Demographic and social data were recorded, as were skin and joints clinical examination. HLA-B27 was tested. All...

  13. Induction of HLA-B27 heavy chain homodimer formation after activation in dendritic cells

    Santos, Susana G.; Lynch, Sarah; Campbell, Elaine C.; Antoniou, Antony N.; Simon J Powis

    2008-01-01

    Introduction Ankylosing spondylitis (AS) is a severe, chronic inflammatory arthritis, with a strong association to the human major histocompatibilty complex (MHC) class I allele human leucocyte antigen (HLA) B27. Disulfide-linked HLA-B27 heavy-chain homodimers have been implicated as novel structures involved in the aetiology of AS. We have studied the formation of HLA-B27 heavy-chain homodimers in human dendritic cells, which are key antigen-presenting cells and regulators of mammalian immun...

  14. Ankylosing spondylitis and HLA-B27: restriction fragment length polymorphism and sequencing of an HLA-B27 allele from a patient with ankylosing spondylitis

    1993-01-01

    Two groups of patients with ankylosing spondylitis (AS) from England and Poland were examined for restriction fragment length polymorphisms (RFLPs) associated with the disease. No preferential association was found between the 9.2 kb PvuII fragment in HLA-B27 positive patients with AS compared with HLA-B27 healthy subjects as had been previously reported. In the English group, however, a 14 kb PvuII fragment was more common in HLA-B27 positive subjects with AS than in normal controls. Also 4....

  15. Klebsiella 'modifying factor': binding studies with HLA-B27+ and B27- lymphocytes.

    Trapani, J A; McKenzie, I. F.

    1985-01-01

    On the basis that extracts of some klebsiella organisms bind selectively to the lymphocytes of HLA-B27+ individuals and induce the appearance of new antigens, attempts were made to detect the binding of klebsiella products to HLA-B27+ and B27- lymphocytes by a number of different techniques. Firstly, blocking of the binding of two different HLA-B27 specific monoclonal antibodies to HLA-B27+ lymphocytes has been examined following exposure of the lymphocytes to a cell-free culture filtrate fro...

  16. A subset of HLA-B27 molecules contains peptides much longer than nonamers.

    Urban, R G; Chicz, R M; Lane, W S; Strominger, J.L.; Rehm, A.; Kenter, M J; Uytdehaag, F G; Ploegh, H.; Uchanska-Ziegler, B; Ziegler, A.

    1994-01-01

    An unusual monoclonal antibody (MARB4) directed against HLA-B27 that reacts with only approximately 5-20% of the cell surface HLA-B27 was used for large-scale purification of these molecules. Subsequent mass spectrometry of HLA-B27-bound peptides showed that the minor MARB4-reactive population contained peptides primarily from 900 to 4000 Da in size (approximately 8-33 amino acid residues), whereas the major HLA-B27 population contained peptides in the mass range of 900-1400...

  17. Mimicry of human histocompatibility HLA-B27 antigens by Klebsiella pneumoniae.

    Ogasawara, M.; Kono, D H; Yu, D T

    1986-01-01

    Anti-HLA-B27 monoclonal antibody M2, which was relatively specific for human histocompatibility antigen HLA-B27, was used to test several bacteria, some of which could potentially induce chronic arthritis in HLA-B27-positive individuals. Using the Western blot procedure, we observed positive reactions with 80,000- and 60,000-dalton antigens with one strain of Klebsiella pneumoniae. Reactivity was not observed with five other monoclonal antibodies which were not reactive with HLA-B27 antigens,...

  18. The arthritis-associated HLA-B*27:05 allele forms more cell surface B27 dimer and free heavy chain ligands for KIR3DL2 than HLA-B*27:09

    Cauli, A; Shaw, J; Giles, J.; Hatano, H; Rysnik, O; Payeli, S.; McHugh, K; Dessole, G; G. Porru; Desogus, E; Fiedler, S.; Hölper, S; CARETTE A.; Blanco-Gelaz, MA; Vacca, A.

    2013-01-01

    OBJECTIVES: HLA-B*27:05 is associated with AS whereas HLA-B*27:09 is not associated. We hypothesized that different interactions with KIR immune receptors could contribute to the difference in disease association between HLA-B*27:05 and HLAB*27:09. Thus, the objective of this study was to compare the formation of β2m-free heavy chain (FHC) including B27 dimers (B272) by HLA-B*27:05 and HLA-B*27:09 and their binding to KIR immunoreceptors. METHODS: We studied the formation of HLA-B*27:05 and H...

  19. The arthritis-associated HLA-B*27:05 allele forms more cell surface B27 dimer and free heavy chain ligands for KIR3DL2 than HLA-B*27:09.

    Cauli, A; Shaw, J; Giles, J.; Hatano, H; Rysnik, O; Payeli, S.; McHugh, K; Dessole, G; G. Porru; Desogus, E; Fiedler, S.; Hölper, S; CARETTE A.; Blanco-Gelaz, MA; Vacca, A.

    2013-01-01

    OBJECTIVES: HLA-B*27:05 is associated with AS whereas HLA-B*27:09 is not associated. We hypothesized that different interactions with KIR immune receptors could contribute to the difference in disease association between HLA-B*27:05 and HLAB*27:09. Thus, the objective of this study was to compare the formation of β2m-free heavy chain (FHC) including B27 dimers (B272) by HLA-B*27:05 and HLA-B*27:09 and their binding to KIR immunoreceptors. METHODS: We studied the formation of HLA-B*27:05 and H...

  20. THE CLINICAL SIGNIFICANCE OF ANTIGEN DETECTION OF HLA-B27%HLA-B27抗原检测的临床意义

    张向阳; 郑海萍; 门金娥

    2008-01-01

    [目的]探讨HLA-B27抗原检测的临床意义.[方法]使用流武细胞仪对350名随机人群、356名强直性脊柱炎(AS)疑似患者和70名AS患者进行HLA-B27抗原检测.[结果]随机人群、AS疑似患者和AS患者的HLA-B27阳性率分别为2.29%、45.79%和91.43%,AS疑似患者的HLA-B27阳性率显著高于随机人群(P<0.01),AS患者的HLA-B27阳性率显著高于AS疑似患者(P<0.01).AS疑似患者中,男性患者的阳性率显著高于女性患者(P<0.01),青年患者的阳性率显著高于老年患者(P<0.01).[结论]在AS疑似患者中,尤其是青年男性,开展HLA-B27抗原检测有着重要的临床意义.

  1. HLA-B27检测对强直性脊柱炎诊断的价值%Diagnostic Value of HLA- B27 for Ankylosing Spondylitis

    沈云; 程涌江; 刘光平

    2001-01-01

    [目的]评价人白细胞抗原b27(HLA-B27)对诊断强直性脊柱炎(AS)的价值.[方法]选择92例AS确诊病人作病例组,70例非AS病人为对照组,均采用美国国立卫生院(NIH)微量淋巴细胞毒试验技术定性检测其HLA-B27含量.[结果]HLA-B27检测对诊断AS的敏感性为87.0%,特异性为91.4%;随机人群试验后HLA-B27阳性的患病概率为2.57%;当患病概率为50%时,试验后HLA-B27阳性患病概率为91.01%.[结论]HLA-B27检测对AS有重要的辅助诊断价值,但不能用作AS的确诊标准.

  2. HLA - B27 polymorphism in AS patients in Shandong%山东地区AS患者HLA-B27基因多态性分析

    李钦伟; 毛永鑫; 李文超; 陈立辉

    2006-01-01

    目的凋查HLA-B27等位基因在山东地区的分布情况,研究亚犁水平的HLA-B27等位基因与AS的相关性.方法应用序列特异性引物聚合酶链反应(PCR-SSP)技术检测山东地区HLA-B27阳性AS患者中B2701~25的等位基因.结果在36例HLA-B27阳性的AS患者中,只检测到B*2704和B*2705两种业型等位基因,其中B*2704阳性13例,B*2705阳性23例,构成比分别是36.11%(13/36),63.89%(23/36).结论山东地区AS患者HLA-B27,等位基因以B*2704和B*2705为主,且B*2705最多见;不同地区、不同人群的AS患者,HLA-B27各亚型分布频率存在差异性.

  3. Gen HLA-B27: polimorfizam, evolucija, raspodjela i povezanost sa spondiloartropatijama

    Grubić, Zorana

    2006-01-01

    Povezanost HLA-B27 i ankilozantnog spondilitisa, odnosno drugih spondiloartropatija, poznata je više od trideset godina. Unatoč velikom broju radova, puno nepoznanica i pitanja još je otvoreno. U ovom su članku iznesena nova saznanja o raznovrsnosti, evoluciji, raspodjeli alela HLA-B27, kao i o hipotezama povezanosti s bolestima.

  4. Review for Disease of the Year: Epidemiology of HLA-B27 Associated Ocular Disorders.

    Kopplin, Laura J; Mount, George; Suhler, Eric B

    2016-08-01

    Acute anterior uveitis is generally recognized as the most common form of uveitis. An association with HLA-B27 is seen in approximately half of cases of acute anterior uveitis. The prevalence of HLA-B27 varies widely between ethnic populations, with an approximate 8-10% prevalence in non-Hispanic whites and lower prevalence in Mexican- (4%) and African- (2-4%) Americans. A group of systemic inflammatory diseases, the spondyloarthropathies, similarly demonstrates a strong association with HLA-B27. The strength of association varies, depending on the specific spondyloarthropathy, with the strongest association found in patients with ankylosing spondylitis. The majority of patients with HLA-B27 associated uveitis will have an underlying spondyloarthropathy. Suspicion for HLA-B27 associated uveitis should prompt a careful clinical history to assess for features of a spondyloarthropathy as the characteristics of any associated uveitis may vary. PMID:27232197

  5. Is there a common pathogenesis in aggressive periodontitis & ankylosing spondylitis in HLA-B27 patient?

    Agrawal, Neeraj; Agarwal, Kavita; Varshney, Atul; Agrawal, Navneet; Dubey, Ashutosh

    2016-05-01

    HLA-B27 is having strong association to ankylosing spondylitis (AS) and other inflammatory diseases collectively known as seronegative spondyloarthropathy. In literature, although the evidence for association between AS and periodontitis as well as AS and HLA-B27 are there but the association of aggressive periodontitis in HLA-B27 positive patient with AS are not there. We hypothesize that there may be a common pathogenesis in aggressive periodontitis and ankylosing spondylitis in HLA-B27 patient. A 27-years-old female presented with the features of generalized aggressive periodontitis and difficulty in walking. On complete medical examination, ankylosing spondylitis was diagnosed with further positive HLA-B27 phenotype and negative rheumatic factor. This report may open up a new link to explore in the pathogenesis of aggressive periodontitis. PMID:27063088

  6. PCR-SSP HLA-B27亚型检测及意义%HLA-B27 subtyping by PCR-SSP method

    张志梅; 武大林

    2004-01-01

    目的对HLA-B27进行亚型检测并探讨其临床意义.方法对38例(AS样本24例;非AS样本14例)血清学确定为HLA-B27阳性的样本采用PCR-SSP方法进行HLA-B27亚型分型.结果38例样本,PCR-SSP分型36例B27阳性,2例B27阴性,误差率为5.26%.36例B27阳性中,B*2704占77.78%(28/36例);24例AS样本与14例非AS样本B*2704所占比例统计学分析无显著差异(x2=0.321,P>0.05).结论中国汉族人HLA-B27亚型以B*2704为主,AS与HLA-B27各亚型的特异基因可能无关.PCR方法准确、实用、快捷、可靠.

  7. Development of a PCR method to detect HLA-B27 in ankylosing spondylitis

    Nätterkvist, Ylva

    2012-01-01

    The aim of the project was to develop a PCR method to detect HLA-B27 at the Immunology Department of St. James hospital in Dublin. The HLA-B27 gene is common among patients with ankylosing spondylitis (AS). Ninety percent of patients with AS have the HLA-B27 gene and it is therefore counted as a risk factor and could be used as part of the diagnosis. Twenty-two frozen blood samples from patients with AS or suspected AS were donated from the rheumatology department at St. James hospital. PCR i...

  8. HLA B-27 Subtypes in Turkish Patients with Spondyloarthropathy and Healthy Controls

    Fatma Savran Oguz; Lale Ocal; Ali Sarper Diler; Hilmi Ozkul; Faruk Asicioglu; Esen Kasapoglu; Gokay Bozkurt; Meral Konice; Mahmut Carin

    2004-01-01

    The frequency and the distribution of HLA-B27 subtypes in spondylarthropathy (SpA) patients and controls were investigated in a sample Turkish population. B27 subtyping was performed by PCR-SSP method in two groups: 49 unrelated HLA-B27 positive Turkish patients with the diagnosis of SpA according to the European Spondyloarthropathy Study Group Criteria, and 55 HLA-B27 positive healthy controls. The frequency of HLA-B∗27 was 2.6% in the Turkish population, and B∗2705 was the predominant allel...

  9. Search for cross-reactivity between HLA B27 and Klebsiella pneumoniae.

    Archer, J R

    1981-01-01

    HLA B27 is associated with a number of forms of arthritis, including ankylosing spondylitis. It has been suggested that the disease is caused by Klebsiella pneumoniae and that the bacterium evokes an odd immune response because it cross-reacts with HLA B27. This proposed cross-reactivity was investigated in a number of ways. The results consistently failed to confirm evidence from cross reaction, even in antisera with activity against both HLA B27-positive lymphocytes and klebsiella. It is su...

  10. Panuveíte em artrite indiferenciada HLA-B27 positiva Panuveitis in HLA-B27 positive undifferentiated arthritis

    Mário Sérgio Ferreira Santos; Vitor Cortizo; Cícero Ricardo Torres da Costa; Ronnielly Melo Tavares; Ricardo Eric Barros Lopes

    2008-01-01

    Entre os vários tipos de inflamação ocular associados às doenças reumatológicas, a uveíte anterior é particularmente comum nas espondiloartropatias, em especial quando associada à presença do genótipo HLA-B27. Relatou-se o caso de um paciente com artrite indiferenciada HLA-B27 positivo, complicado com panuveíte e vasculite da retina, refratária ao tratamento imunossupressor tradicional, que obteve boa resposta clínica ao uso de anti-TNF-alfa.Among the several types of ocular inflammation asso...

  11. Clinical Features and Prognosis of HLA-B27 Positive and Negative Anterior Uveitis in a Korean Population

    Park, Sung Chul; Ham, Don-Il

    2009-01-01

    Clinical features and prognosis of HLA-B27 positive anterior uveitis (AU) were assessed compared with HLA-B27 negative AU in a Korean population, based on the medical records of AU patients seen at a university hospital. Twenty-seven HLA-B27 negative, idiopathic AU patients (group I) and 55 HLA-B27 positive AU patients (group II) were studied. HLA-B27 positive group was further divided into 29 with associated systemic disease (seronegative spondyloarthropathy) (group IIA) and 26 without assoc...

  12. Functional Interaction of the Ankylosing Spondylitis-associated Endoplasmic Reticulum Aminopeptidase 1 Polymorphism and HLA-B27 in Vivo*

    García-Medel, Noel; Sanz-Bravo, Alejandro; Nguyen, Dung; Galocha, Begoña; Gómez-Molina, Patricia; Martín-Esteban, Adrián; Alvarez-Navarro, Carlos; de Castro, José A. López

    2012-01-01

    The association of ERAP1 with ankylosing spondylitis (AS)1 among HLA-B27-positive individuals suggests that ERAP1 polymorphism may affect pathogenesis by altering peptide-dependent features of the HLA-B27 molecule. Comparisons of HLA-B*27:04-bound peptidomes from cells expressing different natural variants of ERAP1 revealed significant differences in the size, length, and amount of many ligands, as well as in HLA-B27 stability. Peptide analyses suggested that the mechanism of ERAP1/HLA-B27 in...

  13. 两种方法检测HLA-B27的结果分析

    廖艳秋; 王红梅; 周建华

    1999-01-01

    HLA-B27与强直性脊椎炎(AS)、Reiter病、急性前葡萄膜炎等疾病相关,我国强直性脊椎炎患者中,91%带有HLA-B27抗原,而正常人群带有HLA-B27抗原的只占6%,对于临床上怀疑为AS的患者,检查HLA-B2,有诊断及鉴别诊断的价值。过去检测HLA-B27只能依赖血清学方法,我们引进了PCR方法.对27例标本同时采用两种方法进行了检测,现将结果报告如下。

  14. HLA-B27 allele frequency in Sri Lankan patients with spondyloarthritides.

    Kidnapillai, S; Sirisena, N D; Dissanayake, V H

    2016-06-01

    This preliminary study aims to describe the HLA-B27 allele frequency in Sri Lankan patients with spondyloarthritides (SA). An anonymised database of 373 Sri Lankan patients with SA referred for HLA-B27 testing was retrospectively analysed. Eighty five (22.8%) patients were positive for the HLA-B27 allele. A male preponderance was observed among the positives. The HLA-B27 allele frequency in this sample of patients with SA was relatively low compared to published studies in other populations. Further research is needed to identify the predominant subtypes of the allele to determine which subtypes are the most prevalent in a larger sample of Sri Lankan patients with SA, and to define their association with the specific types of SA. PMID:27423748

  15. Prevalence of HLA-B27 in Patients with Ankylosing Spondylitis in Qatar

    M. H. Abdelrahman; Mahdy, S.; I. A. Khanjar; A. M. Siam; H. A. Malallah; S. A. Al-Emadi; Sarakbi, H. A.; M. Hammoudeh

    2012-01-01

    Background and Objectives. The human leukocyte antigen HLA-B27 is a class 1 antigen of the major histocompatibility complex and is strongly associated with ankylosing spondylitis (AS). The purpose of the present study is to investigate the distribution of HLA-B27 in patients with AS of different ethnic groups in Qatar. Design and Setting. Study design was cross-sectional and the setting was rheumatology clinics of Hamad General Hospital in Qatar where most of ankylosing spondylitis patien...

  16. Is the HLA B27 genotype a risc faktor for psoriatic arthritis and psoriasis vulgaris?

    Zerrin Öğretmen; Merve Meliha Hız; Fatma Sılan; Şule Koşar; Öztürk Özdemir

    2014-01-01

    Backround and Design: Psoriasis is a common inflammatory dermatological disease which may be complicated with joint involvement. It has been suggested that there is an association between HLA-B27 positivity and early onset psoriasis. The purpose of the current study was to investigate the incidence of HLA-B27 positivity in psoriasis patients with arthritis. Materials and Methods: In a total of 96 patients with psoriasis, age of onset, family history, and Psoriasis Area and Severity Index (...

  17. Prevalence of HLA-B27 antigen in patients with juvenile idiopathic arthritis

    Żuber, Zbigniew; Turowska-Heydel, Dorota; Sobczyk, Małgorzata; Chudek, Jerzy

    2015-01-01

    Introduction Human leukocyte antigen B27 (HLA-B27) is considered as a risk factor for development of juvenile idiopathic arthritis (JIA). The aim of this study was to analyse the prevalence of HLA-B27 antigen in JIA categories and its influence on disease onset and response to conventional therapy. Material and methods The retrospective analysis included 461 unselected children with JIA hospitalized in a single reference rheumatology centre between July 2007 and June 2012. The diagnosis was b...

  18. HLA B27 allele types in homogeneous groups of juvenile idiopathic arthritis patients in Latvia

    Guseinova Dinara

    2010-10-01

    Full Text Available Abstract Juvenile idiopathic arthritis (JIA is a heterogeneous condition and therapeutic strategies vary in different JIA types. The routinely accepted practice to start with Sulphasalazine (SS as the first line treatment in patients with HLA B27 positive JIA proves to be ineffective in a large proportion of children. Objective to investigate HLA B27 positive JIA patients clinical characteristics, determined HLA B27 allele types and their connection with antirheumatic treatment in homogenous patient groups. Materials and methods 56 patients diagnosed with JIA and observed over the period 2006 to 2009 included in the study. HLAB27 allele types were determined using PCR method. Results In HLA B27 positive JIA patients mean disease onset was 12.34 ± 3.3 years. Most common (44% JIA type was enthesitis related arthritis. Positive response to the treatment with SS was found in 32% of patients, Methotrexate (MTX - in 43%, combined treatment - SS with MTX was effective in 12.5%. 12.5% of patients required combination MTX with Enbrel. Eight HLA B27 allele types were found in JIA patients in Latvia: *2702, *2703, *2704, *2705, *2710, *2715, *2717, *2728. The most common was *2705 - in 55% of cases. Among all the patients enthesitis related arthritis most commonly occurred in patients with HLAB*2705 allele (OR = 2.01, p Conclusions There are 8 different HLA B27 alleles in JIA patients in Latvia and the most common is *2705, but in order to assert them to be disease associated alleles, more extensive studies are needed, including control group of HLA B27 positive healthy individuals. Standard treatment approach with SS proves to be unsatisfactory in the majority of JIA patients. To improve children's quality of life achieving rapid disease control, the first line treatment in HLA B27 positive patients should be MTX. In order to start with the most appropriate drug it is necessary to determine HLAB 27 type at the onset of disease.

  19. Panuveíte em artrite indiferenciada HLA-B27 positiva Panuveitis in HLA-B27 positive undifferentiated arthritis

    Mário Sérgio Ferreira Santos

    2008-10-01

    Full Text Available Entre os vários tipos de inflamação ocular associados às doenças reumatológicas, a uveíte anterior é particularmente comum nas espondiloartropatias, em especial quando associada à presença do genótipo HLA-B27. Relatou-se o caso de um paciente com artrite indiferenciada HLA-B27 positivo, complicado com panuveíte e vasculite da retina, refratária ao tratamento imunossupressor tradicional, que obteve boa resposta clínica ao uso de anti-TNF-alfa.Among the several types of ocular inflammation associated to the rheumatic diseases, anterior uveitis is particularly common in the spondyloarthropathies, especially when associated to the presence of the HLA-B27 genotype. We report the case of HLA-B27 positive patient with undifferentiated arthritis, complicated with panuveitis and retinal vasculitis, that was refractory to the traditional imunossupressive treatment, and had a good clinical response with anti-TNF-alpha therapy.

  20. HLA-B27在门诊疑似AS诊断中的意义

    杨栋梁; 邢卫高

    2012-01-01

    目的讨论门诊疑似强制性脊柱炎(AS)诊断中HLA-B27基因检测的意义。方法对门诊疑似诊断为AS的889例患者的HLA-B27阳性率、患者性别和初诊年龄进行统计分析,并与同期随机采集的506例健康人的HLA-B27阳性率进行比较。结果 889例门诊疑似AS患者中HLA-B27阳性率为44.77%(398/889),其中男性患者的HLA-B27阳性率47.55%(339/713)与女性患者的35.52%(59/176)相比,差异具有显著统计学意义(χ2=11.23,P<0.01);对照组中HLA-B27阳性率为4.15%(21/506),与疑似AS患者的阳性率相比,呈显著统计学差异(χ2=253.18,P<0.001);男性患者发病高峰为11-20岁,而女性患者则为31-40岁。结论疑似AS患者就诊时检测HLA-B27有助于提高初诊患者诊断率,可作为鉴别诊断的重要指标。

  1. HLA-B27在门诊疑似AS诊断中的意义

    杨栋梁; 邢卫高

    2012-01-01

    目的 讨论门诊疑似强制性脊柱炎(AS)诊断中HLA-B27基因检测的意义.方法 对门诊疑似诊断为AS的889例患者的HLA-B27阳性率、患者性别和初诊年龄进行统计分析,并与同期随机采集的506例健康人的HLA-B27阳性率进行比较.结果 889例门诊疑似AS患者中HLA-B27阳性率为44.77%(398/889),其中男性患者的HLA-B27阳性率47.55%(339/713)与女性患者的35.52%(59/176)相比,差异具有显著统计学意义(χ2=11.23,P<0.01);对照组中HLA-B27阳性率为4.15%(21/506),与疑似AS患者的阳性率相比,呈显著统计学差异(χ2=253.18,P<0.001);男性患者发病高峰为11-20岁,而女性患者则为31-40岁.结论 疑似AS患者就诊时检测HLA-B27有助于提高初诊患者诊断率,可作为鉴别诊断的重要指标.

  2. 相对定量检测HLA-B27 mRNA表达水平的方法建立%Establishment of a method for relatively quantitative measurement of HLA-B27 mRNA

    王静成; 王晓玲; 费文勇; 郭开今; 王强; 王骅; 陈伟华

    2009-01-01

    Objective To establish a method for relatively quantitative measurement of HLA-B27 mRNA.Methods Totally 27 cases of peripheral venous blood samples were collected from patients with ankylosing spondylitis,including 22 cases from the patients with HLA-B27 positive and 5 cases with HLA-B27 negative.Total RNA from the venous blood samples was extracted,and then the expression level of HLA-B27 mRNA was quantitatively detected by using reverse transcription polymerase chain reaction(RT-PCR).Results The expression level of HLA-B27 mRNA was higher in patients with HLA-B27 positive(n=22;1.16-37.01,mean value was 7.77)than that in patients with HLA-B27 negative(n=5;mean value was 1.19,close to that of healthy control group).Conclusion Relatively quantitative measurement of HLA-B27 mRNA is helpful for monitoring of ankylosing spondylitis.%目的 建立检测HLA-B27 mRNA表达水平的相对定量的方法.方法 筛选未经治疗的AS患者外周静脉血27例,其中HLA-B27阳性患者22例,HLA-B27阴性患者5例,对标本进行Total RNA提取.然后用RT-PCR方法进行HLA-B27反转录及相对定量.结果 22例HLA-B27阳性AS患者有着相对较高的HLA-B27 mRNA表达水平,最低值为1.16,最高值为37.01,均值为7.77.5例HLA-B27阴性AS患者均值为1.19,接近于HLA-B27阴性健康对照组.结论 检测外周静脉血HLA-B27 mRNA表达水平对检测AS是一种有效可行的方法.

  3. Is the HLA B27 genotype a risc faktor for psoriatic arthritis and psoriasis vulgaris?

    Zerrin Öğretmen

    2014-09-01

    Full Text Available Backround and Design: Psoriasis is a common inflammatory dermatological disease which may be complicated with joint involvement. It has been suggested that there is an association between HLA-B27 positivity and early onset psoriasis. The purpose of the current study was to investigate the incidence of HLA-B27 positivity in psoriasis patients with arthritis. Materials and Methods: In a total of 96 patients with psoriasis, age of onset, family history, and Psoriasis Area and Severity Index (PASI values were recorded. The patients were evaluated with regard to physical examination (presence of arthritis, acute phase reactants, HLA-B27 positivity and joint radiographs. Control group comprised of 100 randomly selected healthy individuals. Results: Thirty (31.250% patients were with psoriasis alone, 66 (68.75% were with the findings of psoriasis and arthritis. Of the 66 patients, 17 (17.708% were symptomatic (clinical and radiologic findings and 49 (51.042% subjects were asymptomatic (radiologic findings only. Nine patients (6 with psoriasis only and 3 with psoriatic arthritis and 2 healthy controls were positive for HLA-B27. Conclusion: To carry HLA-B27 antigen increased the risk of psoriasis with an OR of 5.06, and clinically proven psoriatic arthritis with an OR of 10.5 compared to healthy controls. These results need confirmation in a larger group of patients with the inclusion of proper positive and healthy controls.

  4. Association between Polymorphism of the Vitamin D Metabolism Gene CYP27B1 and HLA-B27-Associated Uveitis. Is a State of Relative Immunodeficiency Pathogenic in HLA B27-Positive Uveitis?

    Gernot Steinwender; Ewald Lindner; Martin Weger; Sophie Plainer; Wilfried Renner; Navid Ardjomand; Yosuf El-Shabrawi

    2013-01-01

    OBJECTIVE: Polymorphisms of the vitamin D metabolism gene CYP27B1 showed associations with multiple autoimmune diseases. The aim of this study was to investigate a possible association between the rs703842 A>G polymorphism of the CYP27B1 gene and HLA-B27-associated uveitis. DESIGN: One hundred fifty-nine patients with HLA-B27-associated uveitis, 138 HLA-B27-negative controls and 100 HLA-B27-positive controls were recruited for this retrospective case-control study. Main outcome parameters wer...

  5. A Survey on the Frequency of HLA-B27 in Patients Engaged With Seronegative Spondyloarthropathies in Kashan, Iran

    Kamal Esalat-Manesh; Mohsen Taghadosi; Abas Arj

    2015-01-01

    Background: Seronegative Spondyloarthropathies (SNSAs) are referred to a group of diseases with same clinical and genetic features. Objectives: Due to lack of data about HLA-B27 prevalence in patient with SNSA in Kashan this study was conducted to determine the status of HLA-B27 in these patients. Patients and Methods: This cross sectional study was conducted on 294 patients suffered from SNSA. Presence or absence of HLA-B27 was checked by serological method using Greener kit. Res...

  6. The radiographic features of rheumatoid arthritis in HLA-B27-positive patients

    Rundback, J.H. (Dept. of Radiology, Beth Israel Medical Center, New York, NY (United States)); Rosenberg, Z.S. (Dept. of Radiology, Hospital for Joint Diseases, Orthopaedic Inst., New York, NY (United States)); Solomon, G. (Dept. of Rheumatology, Hospital for Joint Diseases, Orthopaedic Institute, New York, NY (United States))

    1993-05-01

    Radiographs were reviewed in a group of nine patients with classical seropositive rheumatoid arthritis who on tissue typing were found to express the class I HLA-B27 allele. Radiographs were analyzed with regard to whether or not they demonstrated radiographic features of (1) classical rheumatoid arthritis, (2) seronegative arthritis, or (3) mixed features of rheumatoid and seronegative arthritis. Five patients (55%) displayed radiographic features consistent with a diagnosis of rheumatoid arthritis, two patients (22%) showed radiographic features of seronegative disorder (periostitis and sacroiliitis), and two patients (22%) showed a mixed picture with evidence of both rheumatoid arthritis and a seronegative disorder. Thus, the HLA-B27 allele contributed to the radiographic features in 44% of patients with rheumatoid arthritis and associated HLA-B27. Thus, the wide range of findings in our population indicates that the radiographic attributes are not specific enough to constitute a unique subpopulation of patients with rheumatoid arthritis. (orig.)

  7. The radiographic features of rheumatoid arthritis in HLA-B27-positive patients

    Radiographs were reviewed in a group of nine patients with classical seropositive rheumatoid arthritis who on tissue typing were found to express the class I HLA-B27 allele. Radiographs were analyzed with regard to whether or not they demonstrated radiographic features of (1) classical rheumatoid arthritis, (2) seronegative arthritis, or (3) mixed features of rheumatoid and seronegative arthritis. Five patients (55%) displayed radiographic features consistent with a diagnosis of rheumatoid arthritis, two patients (22%) showed radiographic features of seronegative disorder (periostitis and sacroiliitis), and two patients (22%) showed a mixed picture with evidence of both rheumatoid arthritis and a seronegative disorder. Thus, the HLA-B27 allele contributed to the radiographic features in 44% of patients with rheumatoid arthritis and associated HLA-B27. Thus, the wide range of findings in our population indicates that the radiographic attributes are not specific enough to constitute a unique subpopulation of patients with rheumatoid arthritis. (orig.)

  8. HLA-B27 detection – comparison of genetic sequence-based method and flow cytometry assay

    Skalska, Urszula; Kozakiewicz, Anna; Maśliński, Włodzimierz; Jurkowska, Monika

    2015-01-01

    Objectives The presence of human leukocyte antigen B27 (HLA-B27) is strongly associated with ankylosing spondylitis. HLA-B27 testing is routinely applied in the diagnosis of this disease. The aim of the present study was to compare two methods of HLA-B27 detection – a genetic sequence-based method and a flow cytometry assay. Material and methods Peripheral blood was obtained from 300 individuals with suspected spondyloarthropathy. Expression of HLA-B27 on the T cell surface was analysed by fl...

  9. HLA-B27 Selects for Rare Escape Mutations that Significantly Impair Hepatitis C Virus Replication and Require Compensatory Mutations

    Neumann-Haefelin, Christoph; Oniangue-Ndza, Cesar; Kuntzen, Thomas; Schmidt, Julia; Nitschke, Katja; Sidney, John; Caillet-Saguy, Célia; Binder, Marco; Kersting, Nadine; Kemper, Michael W.; Power, Karen A.; Ingber, Susan; Reyor, Laura L.; Hills-Evans, Kelsey; Kim, Arthur Y.

    2011-01-01

    HLA-B27 is associated with spontaneous viral clearance in hepatitis C virus (HCV) infection. Viral escape within the immunodominant HLA-B27 restricted HCV-specific CD8+ T cell epitope NS5B2841-2849 (ARMILMTHF) has been shown to be limited by viral fitness costs as well as broad T cell cross-recognition, suggesting a potential mechanism of protection by HLA-B27. Here, we studied the subdominant HLA-B27 restricted epitope NS5B2936-2944 (GRAAICGKY) in order to further define the mechanisms of pr...

  10. Determination of HLA-B27 Subtypes in Iranian Patients with Ankylosing Spondylitis

    Mohamad Hossein Nicknam

    2008-03-01

    Full Text Available The human leukocyte antigen-B27 is one of the class I molecules of the major histocompatibility complex which is strongly associated with ankylosing spondylitis (AS. The strength of the disease association with B27 varies markedly among racial and ethnic populations. It is an allele family, which constitutes about 31 subtypes, with a considerable geographic and ethnic difference in distribution. It is important to know whether certain subtypes show any preferential association with AS. Because there is no report regarding HLA-B27 subtypes in Iranian patients with AS, the factthe main there are rarelystudies (if any; purpose of the present study was to assess the frequency of subtypes of human leukocyte antigen (HLA-B27 in patients with ankylosing spondylitis in Iranian populationOne hundred and nineteen AS patients (82 HLA-B27 positive and 37 HLA-B27 negative were selected for this study. HLA-B27 positive patients were selected screened for B*27 subtyping were performed by polymerase chain reaction amplification with sequence-specific primers (PCR-SSP for B*27 subtyping.. The results of present study revealed that onlythat only two subtypes were detected in Iranian patients, including: B*2705 (52 patients, 63.4% and B*2702 (30 patients, 36.6%. Our results showed a restricted number of HLA-B27 subtypes associated with AS in Iran and an elevated frequency of the B*2705 allele in these patients similar to other Euro-Caucasoid (Aryan groups in the world.

  11. Clinical Characteristics of Patients with Spondyloarthritides and HLA-B27 Positive Antigen

    Glasnović, Marija; Bošnjak, Ivica; Šram, Miroslav; Vranješ, Željko; Včev, Aleksandar; Dobrošević, Blaženka; Sinčić Petričević, Jasminka; Horvatić, Elizabeta; Orkić, Želimir; Tadžić, Refmir; Soldo, Anamarija; Šišljagić, Dina; Dinjar, Kristijan

    2011-01-01

    The aim of this study was to present our experiences in diagnosing spondyloarthritides (SpA), and to list the most common clinical features of HLA-B 27 positive patients.The study included 65 HLA-B 27 positive patients with confirmed diagnosis of ankylosing spondylitis(AS) and psoriatic arthritis (PsA) who were analyzed between 2009 and 2010 in Clinic of Internal Medicine in Osijek. The diagnosis of seronegative spondyloarthritides was based on the ASAS (Assessment in AS Working G...

  12. HLA-B27与强直性脊柱炎的相关性研究进展%Research progress in the relationship between HLA-B27 and ankylosing spondylitis

    方懿; 蒋宗滨

    2011-01-01

    Background Ankylosing Spondylitis (AS) is a common autoimmune joint disease, which mostly occurs to young men. Early diagnose of AS is difficult and the treatment is not satisfactory at present. Studies have been released that there is a strong relationship bewteen Human leucocyte antigen-B27 (HLA-B27). It is estimated that 90% AS patients were HLA-B27 positive, which was thought of vital importance for early diagnosis of AS. Objective This paper will review the relationship between AS and HLA -B27 which would provide the base on diagnosis and treatment of AS. Content This paper focuses on AS pathogenesis, pathology and clinical manifestations to summarize the relationship and diagnosis significance between HLA -B27 and AS. Trend The HLA-B27 antigen has a high correlation with AS, which can be used as an important clinical indicator for early diagnosis of AS. However it should be mentioned that any exaggeration of the role of HLA-B27 in the diagnosis of AS should be avoided, particular in HLA-B27 negative patients.%背景 强直性脊柱炎(ankylosing spondylitis,AS)是一种常见的自身免疫性关节病,好发于年轻男性,该疾病目前早期诊断较闲难,治疗效果不理想,以往研究表明HLA-B27与AS有很强的相关性,90%左右的AS患者HLA-B27呈阳性,但是仍有10%的患者HLA-B27呈阴性,值得临床进一步研究.目的 现对HLA-B27与AS之间的相关性作一综述,以求为AS的临床诊断和治疗提供帮助.内容 从AS的发病机制、病理学改变和临床表现入手,总结HLA-B27与AS的关系及其对AS诊断的意义.趋向 HLA-B27 抗原的榆测与AS有很高的相关性,可作为临床上早期诊断的重要指标之一,但不能夸大HLA-B27的诊断作用尤其是HLA-B27阴性的患者.

  13. 流式细胞术检测HLA-B27抗原的表达及意义

    吕程; 刘宇; 蒋洪昆

    2008-01-01

    目的 通过流式细胞术对外周血白细胞HLA-B27抗原的检测,了解腰背疼痛和/或关节疼痛患者HLA-B27抗原的分布情况,探讨其在风湿性疾病中的临床应用价值.方法 采用流式细胞仪检测腰背疼痛和/或关节疼痛患者372例HLA-B27抗原的表达.结果 327例患者中HLA-B27阳性163例,总阳性率为43.82%,其中男性259例,HLA-B27阳性137例,阳性率为52.90%.女性113例,HLA-B27阳性26例,阳性率为23.00%.HLA-B27阳性患者年龄分布以青少年为主,12~40岁HLA-B27阳性患者141例,阳性率为37.9%,41~78岁HLA-B27阳性患者22例,阳性率为5.99%.结论 HLA-B27阳性患者以青少年男性为主.HLA-B27检测对各种关节性疾病尤其是脊柱关节性疾病的早期诊断与鉴别诊断具有重要的临床意义.

  14. Clinical Value of HLA-B27 in Ankylosing Spondylitis%人白细胞抗原HLA-B27在强直性脊柱炎中的临床意义

    蒋娟; 熊小敏; 张卫云; 李薇

    2014-01-01

    目的:探讨人白细胞抗原HLA-B27在强直性脊柱炎(AS)中的临床意义,及联合检测HLA-B27、C反应蛋白(CRP)和红细胞沉降率(ESR)的意义.方法:对18例AS患者、24例腰背痛患者和21健康体检者的血液标本进行HLA-B27、CRP、ESR检测,并比较其HLA-B27阳性率.结果:与健康对照组比较,AS组的3项指标均高于对照组(P<0.05),且AS组HLA-B27阳性率均高于另外2组(P<0.05).结论:HLA-B27在强直性脊柱炎诊断中具有重要意义,与其具有高度疾病相关性,联合检测HLA-B27及CRP比联合检测HLA-B27及ESR更有利于疾病诊断.

  15. Association between polymorphism of the vitamin D metabolism gene CYP27B1 and HLA-B27-associated uveitis. Is a state of relative immunodeficiency pathogenic in HLA B27-positive uveitis?

    Gernot Steinwender

    Full Text Available OBJECTIVE: Polymorphisms of the vitamin D metabolism gene CYP27B1 showed associations with multiple autoimmune diseases. The aim of this study was to investigate a possible association between the rs703842 A>G polymorphism of the CYP27B1 gene and HLA-B27-associated uveitis. DESIGN: One hundred fifty-nine patients with HLA-B27-associated uveitis, 138 HLA-B27-negative controls and 100 HLA-B27-positive controls were recruited for this retrospective case-control study. Main outcome parameters were genotype distribution and allelic frequencies determined by polymerase chain reaction. RESULTS: Carriers of the rs703842G allele were found significantly more often in patients with HLA-B27-associated uveitis than in HLA-B27-positive controls (p = 0.03. Between patients and HLA-B27-negative controls no significant difference in the genotype distribution of the rs703842 A>G polymorphism was found (p = 0.97. CONCLUSIONS: Our data suggest that the rs703842 A>G polymorphism may play a role in HLA-B27-associated uveitis.

  16. HLA-B27检测在AS诊断中的临床应用

    昝海波; 王波

    2005-01-01

    目的:评价HLA-B27检测在强直性脊柱炎(AS)诊断中的临床诊断价值.方法:对比分析28例HLA-B27阴性AS患者和56例病程相同的HLA-B27阳性AS患者(1:2配对)的临床表现及实验室检查结果.结果:B27(-)组与B27(+)组最明显的差异是前者以女性多见(P<0.01),平均发病年龄偏晚(P<0.05);病情活动性指标的改变包括血沉和C-反应蛋白的升高在B27(-)组明显少于B27(+)组(P均<0.01).结论:B27(-)AS患者病情相对较轻,预后较好;女性患者病情较轻,发展较慢,预后较好.HLA-B27对AS有一定早期诊断价值,并与血沉、X线片联合检测可提高诊断正确率.

  17. Punctate palmoplantar keratoderma associated with morbus Bechterew and HLA B 27. A family study.

    Gamborg Nielsen, P

    1988-01-01

    Four patients in a family with punctate palmoplantar keratoderma (Buschke-Fischer) associated with Morbus Bechterew and HLA B 27 in 3 of the family members are reported. Without severe side effect, the proband was successfully treated with 50 mg etretinate per day for 6 weeks. PMID:2459882

  18. Use of HLA-B27 tetramers to identify low-frequency antigen-specific T cells in Chlamydia-triggered reactive arthritis.

    Appel, H; Kuon, W; Kuhne, M; P. Wu; Kuhlmann, S.; Kollnberger, S.; Thiel, A.; Bowness, P.; Sieper, J

    2004-01-01

    Reports of the use of HLA-B27/peptide tetrameric complexes to study peptide-specific CD8+ T cells in HLA-B27+-related diseases are rare. To establish HLA-B27 tetramers we first compared the function of HLA-B27 tetramers with HLA-A2 tetramers by using viral epitopes. HLA-B27 and HLA-A2 tetramers loaded with immunodominant peptides from Epstein–Barr virus were generated with comparable yields and both molecules detected antigen-specific CD8+ T cells. The application of HLA-B27 tetramers in HLA-...

  19. Use of HLA-B27 tetramers to identify low-frequency antigen-specific T cells in Chlamydia-triggered reactive arthritis.

    Appel, H; Kuon, W; Kuhne, M; P. Wu; Kuhlmann, S.; Kollnberger, S.; Thiel, A.; Bowness, P.; Sieper, J

    2004-01-01

    Reports of the use of HLA-B27/peptide tetrameric complexes to study peptide-specific CD8+ T cells in HLA-B27+-related diseases are rare. To establish HLA-B27 tetramers we first compared the function of HLA-B27 tetramers with HLA-A2 tetramers by using viral epitopes. HLA-B27 and HLA-A2 tetramers loaded with immunodominant peptides from Epstein-Barr virus were generated with comparable yields and both molecules detected antigen-specific CD8+ T cells. The application of HLA-B27 tetramers in HLA-...

  20. 流式细胞术检测HLA-B27及其临床意义%Detection of HLA-B27 by flow cytometry and it's clinical evaluation

    金明威; 陈哲; 陈颖

    2011-01-01

    Objective: To study the clinical evaluation of HLA -B27 detection by flow cytometry (FCM) for diagnosing ankylostomiasis.Methods: 41 patients with AS, 62 patients with lumbago and 112 normal people were analyzed by FCM.Results: HLA -B27 were positive in 38 (92.6% ); among 41 patients with AS, 6 (9.67% ) of 62 patients with lumbago and 2 ( 1.78% ) of 112 normal persons.Conclusions: The positive rate of HLA - B27 in patients with AS is obviously higher than that of other groups.The detection of HLA - B27 by FCM can play an important role in diagnosing AS.%目的 通过流式细胞术检测外周血白细胞HLA-B27的表达,并探讨其时强直性脊柱炎(AS)的诊断价值.方法 利用流式细胞仪(FCM)检测41例AS患者、62例背痛及腰腿痛患者及112例健康体检者的外周血HLA-B27表达.结果 41例AS患者中,HLA阳性38例(92.6%);62例背痛腰腿痛患者中,HLA-B27阳性6例(9.67%);112例健康人群中,HLA-B27阳性2例(1.78%).结论 AS患者HLA-B27阳性率明显增高,HLA-B27检测可视为临床支持AS诊断的重要依据.

  1. 流式细胞仪在 HLA-B27检测中的应用价值

    刘文超; 杨桂强

    2014-01-01

    目的:探讨流式细胞仪在诊断强直性脊柱炎 HLA-B27检测中的应用价值。方法应用流式细胞仪对86例强直性脊柱炎(AS)病人检测 HLA-B27,来判断流式细胞仪在检测 HLA-B27的临床相关性。结果流式细胞仪检测 HLA-B27的阳性率达到了90.7%,无假阳性及假阴性。结论流式细胞仪检测 HLA-B27是临床诊断强直性脊柱炎的重要辅助诊断方法。

  2. STUDY ON THE HEREDITY RELATIONSHIP BETWEEN ANKYLOSING SPONDYLITIS AND HLA-B27%强直性脊柱炎及HLA-B27的遗传倾向研究

    王小敏; 赵英; 蔡俊宏; 吴艳; 符生苗

    2009-01-01

    [目的]通过检测海南籍强直性脊柱炎(AS)及亲属的HLA-B27的表型、基因型及亚型的分布情况,了解海南籍HLA-B27和AS的遗传倾向及发病情况,指导临床治疗,预防AS的发病.[方法]HLA-B27表型检测采用血清学技术,HLA-B27基因型和基因分型检测均采用序列特异性引物聚合酶链式反应(PCR-SSR)技术.[结果]6例海南籍的AS患者及家族成员共86例中B27阳性者患AS的概率为36.7%,其中男女比例约为7.6:1;有AS相关症状者的概率为36.7%,男女比例为1:5.9.43个HLA-B27阳性家庭中一级亲属AS患病率为30.26%,HLA-B27阳性患AS的比率为47.92%,其中男性为68.97%,女性为15.79%,男女比例为1:4.4.无论是父亲还是母亲HLA-B27阳性,其子女HLA-B27的阳性率基本相近.AS患者中HLA-B27阳性者占100%,HLA-B27表型和基因型基本相符,HLA-B27基因分型结果均为HLA-B2704型.[结论]海南地区AS发病率和HLA-B27阳性率都较其他地区高,具有很强的家族遗传倾向,AS发病率男性较女性高发,有AS相关症状者女性高于男性.HLA-B27基因亚型为HLA-B2704.

  3. HLA-B27检测在强直性脊柱炎诊断中的临床价值%The clinical value of HLA-B27 in the diagnosis of ankylosing spondyfitis

    张虹; 顾猛

    2009-01-01

    目的 探讨HLA-B27抗原检测在强直性脊柱炎(AS)早期诊断中的临床价值.方法 使用ELISA法对450例健康体检者(非AS对照组)、462例AS疑似患者(AS疑似组)和80名AS患者(AS组)进行HLA-B27抗原检测.结果 非AS对照组、AS疑似组和AS组的HLA-B27阳性率分别为3.3%、48.9%和92.5%,AS组、AS疑似组的HLA-B27阳性率明显高于非AS对照组(P<0.01),AS组的HLA-B27阳性率明显高于AS疑似组(P<0.01).结论 HLA-B27抗原与AS疾病相关,检测HLA-B27抗原对疑似AS患者的早期诊断有重要意义.

  4. 强直性脊柱炎867例HLA-B27的表达及意义%Clinical significance of HLA-B27 expression in 867 patients with ankylosing spondylitis

    倪吴花; 胡晓霞; 章圣辉; 吴春雷; 吴建波

    2008-01-01

    目的:通过检测临床疑为强直性脊柱炎(AS)患者的HLA-B27表达,探讨HLA-B27抗原的检测在AS早期诊断中的意义.方法:采用流式细胞术对1 807例疑为AS患者外周血进行HLA-B27表达的检测.结果:在867例诊断为AS的患者中,HLA-B27阳性758 例,HLA-B27检测AS的敏感性为87.4%(758/867),特异性为91.9%(864/940).诊断正确率为89.8%结论:AS与HLA-B27阳性高度相关,临床疑为AS的患者若加上流式HLA-B27抗原检测结果将对AS的早期诊断起到重要作用.

  5. 强直性脊柱炎检测HLA-B27、ASO、RF、CRP的临床意义%The Clinical Significance of Testing HLA- B27、ASO、RF、CRP in Ankylosing Spondylitis

    范洪; 郑小丽; 侯英荣; 冯秀河

    2004-01-01

    目的探讨HLA-B27、ASO、RF、CRP检测在强直性脊柱炎(AS)诊断与鉴别诊断中的意义.方法抽取EDTA(K2)抗凝血2ml,采用磁珠酵素免疫法检测HLA-B27,采用胶乳凝集法检测ASO、RF、CRP.结果31例正常健康者HLA-B27、ASO、RF、CBP检测结果均为阴性.108例疑似AS患者中,HLA-B27阳性率为30.6%(33/108).33例HLA-B27阳性患者中,ASO阳性率为6.1%(2/33),RF阳性率为3.0%(1/33),CRP阳性率为12.1%(4/33).结论 HLA-B27的检测可明确AS诊断,特别是在不典型AS诊断中具有重要的临床价值.ASO、RF检测可用于AS的鉴别诊断.疑似AS患者HLA-B27阳性者中,其活动期的CBP比率较低.

  6. Molecular mechanism of the susceptibility difference between HLA-B*27:02/04/05 and HLA-B*27:06/09 to ankylosing spondylitis: substitution analysis, MD simulation, QSAR modelling, and in vitro assay.

    Cheng, X; Mei, Y; Ji, X; Xue, Q; Chen, D

    2016-05-01

    The human leukocyte antigen HLA-B27 is directly involved in the disease pathogenesis of ankylosing spondylitis (AS). HLA-B27 has a high degree of genetic polymorphism, with 105 currently known subtypes; the presence of aspartic acid at residue 116 (Asp116) has been found to play an essential role in AS susceptibility. Here, we systematically investigated the molecular mechanism of the susceptibility difference between the AS-associated subtypes HLA-B*27:02/04/05 and AS-unassociated subtypes HLA-B*27:06/09 to AS at sequence, structure, energetic and dynamic levels. In total seven variable residues were identified among the five studied HLA-B27 subtypes, in which Asp116 can be largely stabilized by a spatially vicinal, positively charged His114 through a salt bridge, while five other variable residues seem to have only a marginal effect on AS susceptibility. We also employed a quantitative structure-activity relationship approach to model the statistical correlation between peptide structure and affinity to HLA-B*27:05, a genetic ancestor of all other HLA-B27 subtypes and associated strongly with AS. The built regression predictor was verified rigorously through both internal cross-validation and external blind validation, and was then employed to identify potential HLA-B*27:05 binders from >20,000 cartilage-derived self-peptides. Subsequently, the binding potency of the top five antigenic peptides to HLA-B*27:05 was assayed in vitro using a FACS-based MHC stabilization experiment. Consequently, two (QRVGSDEFK and LRGAGTNEK) out of the five peptides were determined to have high affinity (BL50 = 5.5 and 15.8 nM, respectively) and, as expected, both of them possess positively charged Lys at the C-terminus. PMID:27228481

  7. HLA-B27阳性与阴性未分化脊柱关节病的临床比较%Comparison of undifferentiated spondyloarthropathy patients with positive or negetive HLA-B27

    杨积保; 张慧群; 刘智; 李志军

    2010-01-01

    目的:探讨血清人类白细胞抗原-B27(HLA-B27)阳性与阴性未分化脊柱关节病(undifferentiated spondyloarthropathy,uSpA)患者临床表现及预后的差异,为临床诊疗提供借鉴.方法:根据血清HLA-B27是否阳性将120例uSpA患者分为两组,阳性组78例,阴性组42例.分析HLA-B27阳性组与阴性组的临床表现及相关实验室检查结果,比较两组间的差异;并对部分患者进行3年以上随访观察,了解其病情变化.结果:HLA-B27阳性组患者发病年龄显著早于HLA-B27阴性组(P<0.05);发热、消瘦、疲倦等全身症状表现HLA-B27阳性组显著多于HLA-B27阴性组,且较严重(P<0.05);实验室检查中血沉以HLA-B27阳性组显著为高(P<0.05);骶髂关节X线表现亦以HLA-B27阳性组显著为高(P<0.05).而两组性别及外周关节炎等表现差异无统计学意义(P>0.05).随访发现HLA-B27阳性组3年后发展为强直性脊柱炎者显著多于HLA-B27阴性组(P<0.05).结论:HLA-B27阳性组uSpA患者发病年龄较早,临床症状较重,更有可能发展为强直性脊柱炎.

  8. Autoantibodies to HLA B27 in the sera of HLA B27 patients with ankylosing spondylitis and Reiter's syndrome. Molecular mimicry with Klebsiella pneumoniae as potential mechanism of autoimmune disease

    1987-01-01

    Ankylosing spondylitis (AS) and Reiter's syndrome (RS) both show a strong correlation with the HLA B27 haplotype. We studied whether sharing of homologous amino acid sequences in the HLA B27 antigen with an invading microbe might occur, and if so, what is the biological significance of such homology. In a computer search of the Dayhoff data bank, we found a homology of six consecutive amino acids between HLA B27.1 antigen residues 72-77 and Klebsiella pneumoniae nitrogenase residues 188-193. ...

  9. 强直性脊柱炎与HLA-B27相关性的研究进展%Recent advancement about dependability of ankylosing spondylitis and HLA-B27

    孙翠华; 孟凡杰; 王玉芳; 王健

    2007-01-01

    强直性脊柱炎是一种发病率较高的慢性进展性风湿疾病,与人类白细胞抗原-B27(HLA-B27)密切相关,本文总结了近几年对HLA-B27各方面的研究成果及最新进展,有助于了解HLA-B27在此疾病中的确切作用,并可以辅助临床医师对强直性脊柱炎进行诊治.

  10. Association of HLA-B27 and ERAP1 with ankylosing spondylitis susceptibility in Beijing Han Chinese.

    Zhang, Z; Dai, D; Yu, K; Yuan, F; Jin, J; Ding, L; Hao, Y; Liang, F; Liu, N; Zhao, X; Long, J; Xi, Y; Sun, Y-Y

    2014-05-01

    This study investigated the genetic polymorphisms of HLA-B27, together with polymorphisms on endoplasmic reticulum aminopeptidase 1 (ERAP1), and susceptibility for ankylosing spondylitis (AS) in the Beijing Han population. A case-control study was carried out for 602 AS patient samples and 619 matched controls of Han Chinese. HLA-B27 genotyping was performed by polymerase chain reaction-sequence specific primers (PCR-SSP), and four ERAP1 SNPs (rs27037, rs27980, rs27582, and rs27434) were selected and genotyped on the Sequenom iPlex platform (Sequenom, San Diego, CA). Association analysis was performed using the likelihood ratio χ(2) test. This study identified four HLA-B27 alleles in Beijing Han AS patients, B*27:02, B*27:04, B*27:05, and B*27:07, of which B*27:05 was the most significant geographical different subtype among AS patients in Chinese. Our results confirmed that HLA-B27 was strongly associated with AS (P=1.9 × 10(-150) ), and the most strongly associated alleles were B*27:04, B*27:05, and B*27:02. Our study also confirmed a weak association between ERAP1 (rs27434) and AS. We also observed that for HLA-B*27:02 and HLA-B*27:04 positive AS patients, rs27434 and rs27582 were associated with AS. In contrast, for HLA-B27-negative and HLA-B*27:05-positive AS patients, this association was not observed. This is the first study to show that both B27 and ERAP1 are AS genetic susceptibility genes in Beijing Han. Interactions between ERAP1 and HLA-B*27:02 and B*27:04 may play an important role in the AS pathogenesis. PMID:24666027

  11. IgG and IgA immune response against klebsiella in HLA-B27-associated anterior uveitis.

    Kijlstra, A.; Luyendijk, L; van der Gaag, R; van Kregten, E; Linssen, A; Willers, J M

    1986-01-01

    Enteric infections with Gram-negative bacteria are thought to play an important part in HLA-B27-associated disease such as Reiter's syndrome and reactive arthritis. But the role of bacterial infections in HLA-B27-positive ankylosing spondylitis (AS) and acute anterior uveitis (AU) is still controversial. A special interest has recently been devoted to the role of klebsiella infection in HLA-B27-associated disease. We studied the humoral immune response against a 'cross-reactive' strain of Kle...

  12. Povezanost mikrosatelita HLA i gena HLA-B*27 u bolesnika s psorijatičnim artritisom u hrvatskoj populaciji

    Štimac, Davor; Grubić, Zorana; Štingl, Katarina; Perić, Porin; Ćurković, Božidar; Žunec, Renata

    2011-01-01

    Istraživana je raznovrsnost četiri mikrosatelita HLA (D6S248, D6S2674, D6S2811 i D6S273) u skupini bolesnika s psorijatičnim artritisom (PsA) (N=22) i zdravim osobama (K; N=94) pozitivnima za gen HLA-B*27, te povezanost haplotipskih veza gena HLA-B*27 s PsA. Svi ispitanici bili su prethodno tipizirani za gene HLA-A i -B metodom PCR-SSP i bili su HLA-B*27 pozitivni. Mikrosateliti HLA su analizirani metodom PCR-STR i elektroforezom u ALFexpress sekvenceru. Analiza raspodjele alela mikrosatelita...

  13. Yersinia enterocolitica serotype O:3 alters the expression of serologic HLA-B27 epitopes on human monocytes.

    Wuorela, M; Jalkanen, S; Kirveskari, J; Laitio, P; Granfors, K

    1997-01-01

    The expression of serologic HLA-B27 epitopes on leukocytes of patients with reactive arthritis or ankylosing spondylitis has been shown to be modified in the course of the disease. The purpose of this work was to study whether phagocytosis of arthritis-triggering microbes in vitro alters the expression of HLA-B27 molecules on human antigen-presenting cells and to characterize the underlying mechanisms. Human monocytes and HLA-B27- or HLA-A2-transfected human U-937 cells were exposed to Yersin...

  14. Association of HLA-B27 status and gender with sacroiliitis in patients with ankylosing spondylitis

    Xiong, Jiangbiao; Chen, Jing; Tu, Jianxin; Ye, Wenjing; Zhang, Zhiyong; Liu, Qiaoqiong; Zhu, Xiaochun

    2014-01-01

    Objective: To observe the influence of human leucocyte antigen B27 (HLA-B27) status and gender on sacroiliitis on computed tomography (CT) in ankylosingspondylitis (AS). Methods: We reviewed the archived medical records of the AS inpatients admitted in the Rheumatology Department of the First Affiliated Hospital of Wenzhou Medical University during the period from January 2007 through January 2013 and finally 386 patients were included in the study. The severity of sacroiliitis on CT was eval...

  15. The role of HLA-B27 molecules in the pathogenesis of ankylosing spondylitis

    R. Pala

    2011-09-01

    Full Text Available Ankylosing Spondylitis (AS is characterised by the strongest association with an HLA antigen ever described for any disease. It represents therefore the ideal model for the understanding of the link between immune-mediated diseases and the HLA system. The role of HLA-B27 in the pathogenesis of AS will be discussed focusing on the recently described higher expression of these molecules in patients with AS compared with healthy controls.

  16. Arthrite rhumatoïde juvénile et HLA-B27.

    Mathon, G; ParÉ, C.; Ménard, H; Tétreault, L; Camerlain, M

    1980-01-01

    Forty children with juvenile rheumatoid arthritis were studied to determine the frequency of the histocompatibility antigen HLA [human leukocyte antigen)-B27 in this disease and to characterize the arthropathy associated with this antigen. HLA-B27 was detected in four patients (10%). Its presence was associated in a statistically significant manner with sacroiliitis demonstrated radiologically and with a greater age at the time symptoms in the joints first appeared; this age was, on average, ...

  17. HLA-B27 Expression Does Not Modulate Intracellular Chlamydia trachomatis Infection of Cell Lines

    Young, J. L.; Smith, L; Matyszak, M. K.; Gaston, J S H

    2001-01-01

    Chlamydia trachomatis is an obligate intracellular pathogen. Infection of susceptible individuals with this bacterium can trigger the development of reactive arthritis, an acute inflammation that is associated with the expression of the class I major histocompatibility antigen, HLA-B27. Other facultative intracellular pathogens, such as Yersinia and Salmonella spp., are also known triggers of reactive arthritis. Previous studies report conflicting results concerning whether the presence of HL...

  18. Antinuclear antibody and HLA-B27 positive uveitis: combination of two diseases ?

    Bosch-Driessen, E.H.; Lardy, N.M.; Rothova, A

    1997-01-01

    AIMS/BACKGROUND—Anterior uveitis associated with juvenile chronic arthritis concerns two different clinical entities: firstly, antinuclear antibody (ANA) positive patients who have a chronic anterior uveitis with severe complications and often a poor visual prognosis; secondly, usually HLA-B27 positive children, predominantly boys, with unilateral recurrent anterior uveitis. Three patients are described who had a combination of clinical and laboratory features of both diseases.
METHODS—Retros...

  19. HLA-B27 positive juvenile arthritis with cardiac involvement preceding sacroiliac joint changes

    Lee, S; Im H, Y; Schueller, W

    2001-01-01

    While cardiovascular disease develops in up to 50% of adult patients with ankylosing spondylitis, it is very uncommon in its juvenile counterpart. Regarding the early stage of the disease, before onset of sacroiliac joint changes, only two cases with aortic incompetence have been published while reports of mitral valve involvement are not available. A 13 year old boy is described with an HLA-B27 positive asymmetric oligoarthritis and enthesitis, without back pain or radiographic evidence of s...

  20. Allicin Attenuates Inflammation and Suppresses HLA-B27 Protein Expression in Ankylosing Spondylitis Mice

    Xin Gu

    2013-01-01

    Full Text Available Here we aimed to determine the therapeutic effect of allicin on ankylosing spondylitis (AS and explore the mechanism(s of action. AS mouse model was constructed by transferring the HLA-B2704 gene into Kunming mice and verified by RT-PCR and CT imaging. Verified AS mice were randomly divided into model group ( and allicin-treated groups (50, 100, and 200 mg/kg, resp., , p.o., for 2 months. Wild type mice were used as control (. The levels of AS-related inflammatory factors were measured by ELISA. mRNA and protein expressions of HLA-B27 were checked by RT-PCR and western blotting. As the results, the mouse model of AS was successfully established, and high-dose allicin could markedly alleviate spine inflammatory injury possibly via reducing the secretion of the inflammatory factors (IL-6, IL-8, and TNF-α sharply in AS mice. Moreover, allicin significantly inhibited HLA-B27 protein translation but failed to suppress HLA-B27 gene transcription in AS mice, indicating a posttranscriptional mechanism of this modulation. In conclusion, allicin has potential to be used for AS treatment as an anti-inflammatory nutraceutical.

  1. Functional variants of ERAP1 gene are associated with HLA-B27 positive spondyloarthritis.

    Cherciu, M; Popa, L O; Bojinca, M; Dutescu, M I; Bojinca, V; Bara, C; Popa, O M

    2013-09-01

    We investigated two nonsynonymous variants (rs30187 and rs27044) of ERAP1 gene in HLA-B27 positive individuals (150 spondyloarthritis and 108 controls) and in general ankylosing spondylitis (AS) patients (n = 137) vs random controls (n = 139). Both single nucleotide polymorphisms (SNPs) were associated with the risk of spondyloarthritis [odds ratio (OR) 1.80, 95% confidence interval (CI) 1.24-2.62, P = 0.001 for rs30187, OR 1.58, 95% CI 1.07-2.34, P = 0.02 for rs27044]. The CC haplotype was a protective factor (P = 0.002), while the TG haplotype was a risk factor (P = 0.01) for spondyloarthritis. The SNP rs30187 was also associated with the risk of HLA-B27+ AS. For the general group of AS, the carriers of minor alleles showed an increased risk for the disease (OR 1.92, 95% CI 1.17-3.13 for rs30187, OR 1.74, 95% CI 1.08-2.80 for rs27044). This is the first study that shows the association of ERAP1 gene variants and haplotypes with HLA-B27 positive spondyloarthritis. PMID:23800305

  2. The impact of the strength of HLA-B27 positive on seronegtive spondyloarthropathies%HLA-B27阳性的强弱对血清阴性脊柱关节病的影响评估

    陈睿; 宋恒平; 倪凤民; 邵先安; 张皓

    2013-01-01

    目的 探讨HLA-B27的阳性强弱对血清阴性脊柱关节病(SPA)诸病种的诊治影响评估.方法 78 例SPA(血清阴性脊柱关节病)中AS(强直性脊柱炎)32 例,USPA(未分化脊柱关节病)42 例,PSA 3例,ReA1 例,计HLA-B27强阳性4 例,阳性30 例,弱阳性40 例,阴性4 例.结果 随访2耀20年,1例HLA-B27强阳性AS 患者不遵医嘱治疗,6 年后两髋关节损害致残,其他3 例HLA-B27强阳性患者急性发作多次,但病情控制较好,30 例HLA-B27阳性患者中3 例失访,其他按正规抗AS 治疗,病情平稳其中男性28例.40 例HLA-B27弱阳性患者中有2 例在1耀4 年后被确诊为AS,1例被诊断反应性关节炎,且女性有29 例,其他病情平稳,HLA-B27阴性4 例,行抗AS 治疗,骶髂关节炎在玉耀域级之间未进一步发展.结论 HLA-B27强阳性及阳性SPA 多见于男性,病情发展快,难以控制病情,HLA-B27强阳性SPA患者致残率高,HLA-B27弱阳性的SPA、USPA患者多见于女性,症状一般较轻,易于控制.HLA-B27阴性SPA 患者更较少见,病情发展慢,且易于控制发展.

  3. Bilateral macular thickening in mild unilateral anterior uveitis: is HLA-B27 involved?

    Wexler Alexandra

    2012-07-01

    Full Text Available Abstract Background Macular thickening (MT without clinically recognized macular edema has been described in anterior uveitis (AU. Although fellow-eyes of patients have been used as controls in several studies, little is known about macular thickness in these eyes. We studied the rate and extent of MT in both AU-affected and quiescent fellow-eyes of phakic AU patients with good visual acuity (VA. We also assessed macular thickness related to HLA-B27 presence and to recurrence, since these issues have been almost unexplored by previous optical coherence tomography (OCT studies. Methods Patients with AU were prospectively included and macular thickness was measured with OCT initially and on follow up. Macular thickness in patients’ affected eyes (n = 30 as well as in their quiet fellow-eyes (n = 28 was compared with eyes of age- and gender matched controls. Inter-ocular differences in macular thickness between AU affected eyes and their fellow-eyes were assessed in patients (n = 28, also in a subgroup with visual acuity ≥ 0.8 (n = 23 by one-sample Student’s t-tests. Inter-ocular differences were also assessed related to HLA-B27 presence and related to the status of current AU episode (initial or relapse. Results Subclinical MT is present in both quiet fellow-eyes and AU-affected eyes of patients. MT was found in most cases of AU, even in phakic eyes with good VA. There was a larger increase in macular thickness in HLA-B27-positive than in HLA-B27-negative patients. No differences in macular thickness were found between patients with their first AU episode and patients with recurrent episodes. Conclusions MT probably reflects systemic immune-mediated response to the inflammatory disorder in AU, and it is possible that HLA-B27-related factors are involved in the pathogenesis of AU. These observations are in line with and extend the current understanding of the mechanisms behind MT in AU.

  4. VALUE OF FLOW CYTOMETRY DETECTION OF HLA-B27 ANTIGEN IN THE DIAGNOSIS OF AS%流式细胞术检测HLA-B27抗原在AS诊断中的价值

    邱金英

    2009-01-01

    目的 探讨人类白细胞抗原-B27(HLA-B27)检测对强直性脊柱炎(AS)在临床诊断中的价值.方法 应用流式细胞术对567例门诊及住院疑似AS患者外周血进行HLA-B27表达的检测.结果 567例中,HLA-B27阳性检出率为43.39%(246/567).按照纽约标准诊断为AS的252例患者中,HLA-B27阳性率为91.67%(231/252).HLA-B27检测对诊断AS的敏感度为91.67%(221/252),特异度为95.24%(300/315),诊断正确率为93.65%(531/567).结论 HLA-B27和AS有很强的相关性,使用流式细胞术进行检测是较好的方法.

  5. Structural analysis of an HLA-B27 functional variant: identification of residues that contribute to the specificity of recognition by cytolytic T lymphocytes.

    Vega, M A; Ezquerra, A.; S Rojo; Aparicio, P.; Bragado, R; López de Castro, J. A.

    1985-01-01

    The structure of a variant HLA-B27 antigen, B27.2, that is distinguished from the HLA-B27.1 and HLA-B27.3 subgroups by specific cytolytic T lymphocytes has been established by comparative peptide mapping and sequence analysis. There are only three amino acid substitutions between B27.1 and B27.2: aspartate-77, threonine-80, and leucine-81 in HLA-B27.1 are changed to asparagine-77, isoleucine-80, and alanine-81 in HLA-B27.2. These changes account for their single charge difference detectable b...

  6. A comparison of self-reported joint symptoms following infection with different enteric pathogens: effect of HLA-B27

    Schiellerup, P.; Krogfelt, K.A.; Locht, H.

    2008-01-01

    OBJECTIVE: We conducted a case-case comparison study to estimate the attack-rate of reactive joint pain (JPrea) following intestinal infections, and evaluated whether the susceptibility and severity of joint symptoms was associated with the tissue-type HLA-B27. METHODS: Consecutive patients with.......8%); Yersinia, 21 (23.1%); Shigella, 10 (9.8%); and E. coli, 28 (9.7%). There was a significant association between severity of gastroenteritis and development of arthralgia (p = 0.001). The odds ratio (OR) for JPrea in an HLA-B27-positive individual was 2.62 (95% CI 1.67-3.93) for the entire group. A...... significant association between JPrea and HLA-B27 was found for Salmonella, Shigella, and Yersinia; not, however, for Campylobacter and E. coli. HLA-B27-positive patients had a significantly increased risk for severe joint symptoms. CONCLUSION: Our study shows that JPrea after GI infection is positively...

  7. 流式细胞仪检测HLA-B27及其临床意义

    徐建

    2014-01-01

    目的:通过流式细胞术对人体外周血HLA-B27的检测,来评价HLA-B27辅助临床诊断强直性脊柱炎(AS)的应用价值。方法56例强直性脊柱炎患者和51例健康体检者,用荧光标记的抗HLA-B27/CD3单克隆抗体与其外周血(以EDTA-K2抗凝)特异性结合,用流式细胞仪检测其荧光强度来反映细胞表面 HLA-B27抗原的表达。结果从流式细胞术检测显示,56例AS患者中,HLA-B27阳性51例,阳性率为91.1%;51例健康体检者中,HLA-B27阳性3例,阳性率为5.9%。两者存在显著性差异,P<0.01。结论 AS患者HLA-B27阳性率明显高于正常人,所以检测HLA-B27对强直性脊柱炎的诊断与鉴别诊断有着重要的意义。

  8. Ultrasonographic assessment of enthesitis in HLA-B27 positive patients with rheumatoid arthritis, a matched case-only study.

    Antonio Mera-Varela

    Full Text Available INTRODUCTION: HLA-B27 has a modifier effect on the phenotype of multiple diseases, both associated and non-associated with it. Among these effects, an increased frequency of clinical enthesitis in patients with Rheumatoid Arthritis (RA has been reported but never explored again. We aimed to replicate this study with a sensitive and quantitative assessment of enthesitis by using standardized ultrasonography (US. METHODS: The Madrid Sonography Enthesitis Index (MASEI was applied to the US assessment of 41 HLA-B27 positive and 41 matched HLA-B27 negative patients with longstanding RA. Clinical characteristics including explorations aimed to evaluate spondyloarthrtitis and laboratory tests were also done. RESULTS: A significant degree of abnormalities in the entheses of the patients with RA were found, but the MASEI values, and each of its components including the Doppler signal, were similar in HLA-B27 positive and negative patients. An increase of the MASEI scores with age was identified. Differences in two clinical features were found: a lower prevalence of rheumatoid factor and a more common story of low back pain in the HLA-B27 positive patients than in the negative. The latter was accompanied by radiographic sacroiliitis in two HLA-B27 positive patients. No other differences were detected. CONCLUSION: We have found that HLA-B27 positive patients with RA do not have more enthesitis as assessed with US than the patients lacking this HLA allele. However, HLA-B27 could be shaping the RA phenotype towards RF seronegativity and axial involvement.

  9. Aetiology and pathogenesis of reactive arthritis: role of non-antigen-presenting effects of HLA-B27

    Vähämiko, Sanna; Penttinen, Markus A.; Granfors, Kaisa

    2005-01-01

    Spondyloarthropathies are inflammatory diseases closely associated with human leukocyte antigen (HLA)-B27 by unknown mechanisms. One of these diseases is reactive arthritis (ReA), which is typically triggered by Gram-negative bacteria, which have lipopolysaccharide as an integral component of their outer membrane. Several findings in vivo and in vitro obtained from patients with ReA and from different model systems suggest that HLA-B27 modulates the interaction between ReA-triggering bacteria...

  10. HLA-B27 modulates the survival of Salmonella enteritidis in transfected L cells, possibly by impaired nitric oxide production.

    Virtala, M; Kirveskari, J; Granfors, K

    1997-01-01

    Reactive arthritis is triggered by certain microbes that cause primary infections mainly on the gastrointestinal or urogenital mucosa. The disease is strongly associated with HLA-B27. Long persistence of causative microbes or their structures in the body has been thought to have an important role in the pathogenesis of reactive arthritis. This suggests that the elimination of the microbes causing reactive arthritis is ineffective or disturbed in HLA-B27-positive individuals developing this co...

  11. 湖南地区汉族健康人群HLA-B*27基因多态性调查

    谢毓滨; 张钢; 曹丽群; 李涛

    2012-01-01

    目的 调查湖南地区汉族健康人群中HLA-B* 27基因多态性.方法 对3488名湖南汉族健康造血干细胞志愿捐献者进行HLA -B基因测序分型,对HLA-B* 27健康携带者亚型分布情况进行分析.结果 3488名湖南汉族志愿捐献者中,共检出HLA-B*27基因健康携带者99名,在人群中的分布比例为2.84%,男女比例无明显差异.99名健康携带者中,共检出HLA-B*27基因亚型6种,其中以HLA -B*27∶04为主(68/100),其次是HLA-B* 27∶05(18/100),HLA -B*27∶06(7/100),HLA-B* 27∶ 07(3/100),HLA-B* 27∶ 02 (2/100),HLA-B*27:24(2/100).结论 湖南地区汉族健康人群HLA-B* 27基因亚型以B*27∶04、B*27∶05为主.

  12. 强直性脊椎炎患者HLA-B27的检测意义

    张颖新; 王凤菊; 颜敏

    2003-01-01

    目的探讨强直性脊椎炎(AS)患者HLA-B27的检测意义.方法 2002年3月至2002年9月,因关节病前来诊断的患者276例,用单抗板淋巴细胞毒法测其白细胞上的HLA-B27抗原.结果确诊为AS的患者中HLA-B27阳性率96.3%(119/124)显著高于非AS的病人的HLA-B27阳性率2.2%(3/152)(P<0.001),B27阴性AS患者发病年龄高于B27阳性患者,且男性多于女性,男女发病年龄相比无统计学意义.结论 HLA-B27与强直性脊椎炎有很强的关联性,对于长期不明原因的腰腿痛患者结合临床及X线HLA-B27可作为AS的早期诊断和鉴别诊断指标.

  13. HLA-B27检测在强直性脊柱炎中的临床应用

    蒋叙川; 卢峰

    2012-01-01

    目的:探讨HLA-B27抗原检测在强直性脊柱炎(AS)早期诊断中的临床价值.方法:使用流式细胞技术对30例健康体检者、41例AS疑似患者和52名AS患者进行HLA-B27抗原检测.结果:体检对照组、AS疑似组和AS组的HLA-B27阳性率分别为0%、12.2%和90.4%,AS组、AS疑似组的HLA-B27阳性率明显高于体检对照组(P<0.01),AS组的HLA-B27阳性率明显高于AS疑似组(P<0.01).结论:HLA-B27抗原与AS疾病相关,检测HLA-B27抗原对疑似AS患者的早期诊断有重要意义.

  14. HLA-B27 frequency in a group of patients with psoriatic arthritis Freqüência de HLA-B27 em uma amostra de pacientes com artrite psoriática

    Danilo Garcia Ruiz

    2012-12-01

    Full Text Available BACKGROUND: HLA-B27 is associated with spondyloarthritis, a group of diseases that includes psoriatic arthritis. OBJECTIVES: To describe the HLA-B27 frequency in a group of Brazilian patients with psoriatic arthritis and correlate its presence or absence with their clinical manifestations. METHODS: Cross-sectional study with 44 psoriatic arthritis patients of a Rheumatology clinic. Demographic and social data were recorded, as were skin and joints clinical examination. HLA-B27 was tested. All data were processed descriptively and comparatively by appropriate software. Parametric and non parametric tests were used with 5% statistical significance. RESULTS: HLA-B27 was negative in 32 of the 44 patients (72,7%. Most of them were male, Caucasian, living in Rio de Janeiro, with plaque type psoriasis and average age of 52,9 years. There was statistical significant correlation between positive HLA-B27 and male gender (p=0,004. Negative HLA-B27 had a tendency to correlate with hands and wrists arthritis (p=0,07. There was an inverse significant correlation between HLA values and Schöber's test (p=0,02. CONCLUSION: Although HLA-B27 is negative in most of patients, it is significantly associated to male gender and inversely correlated with Schöber's test.FUNDAMENTOS: O HLA-B27 está associado às espondiloartrites, grupo de doenças que engloba, entre outras, a artrite psoriásica. OBJETIVOS: Descrever a freqüência de HLA-B27 em uma amostra de pacientes brasileiros com artrite psoriásica e correlacionar sua presença ou ausência com as manifestações clínicas dos mesmos. MÉTODOS: Estudo transversal avaliando 44 pacientes com artrite psoriásica de um ambulatório de Reumatologia. A avaliação consistia em registro de informações demográficas e sociais, exame clínico da pele e das articulações e pesquisa de HLA-B27. Os dados gerados foram tratados por meio de estatística descritiva e comparativa em Software apropriado. Foram utilizados

  15. Detection and clinical significance of HLA- B27 antigen by flow cytometry%流式细胞术检测HLA-B27抗原的表达及临床意义

    陆春伟; 佟海侠

    2010-01-01

    目的 评价流式细胞仪法检测HLA-B27抗原对强直性脊柱炎(AS)、急性前葡萄膜炎(AAU)的临床诊断价值.方法 用流式细胞仪检测85例AS患者、40例AAU患者、90例背痛和腰腿痛患者以及80例健康对照组的HLA-B27抗原表达.结果 AS组HLA-B27检测阳性率(91.8%)高于背及腰腿痛组(5.6%)和健康体检组(3.8%);AAU组HLA-B27检测阳性率(52.5%)高于健康体检组;背及腰腿痛组HLA-B27检测阳性率与健康对照组比较无意义.结论 流式细胞仪法检测HLA-B27抗原对AS的临床诊断有较高敏感度和特异度,是一种准确、有效的实验诊断方法;对AAU患者进行HLA-B27检测,对评价其临床特征和预后有一定价值.

  16. Phosphorylation of STAT-1 serine 727 is prolonged in HLA-B27-expressing human monocytic cells.

    Marja Ruuska

    Full Text Available A tissue antigen, HLA-B27, is strongly associated with a group of rheumatic diseases called spondyloarthritides. Despite the intensive research, the exact role of HLA-B27 in the pathogenesis of these diseases is still unclear. Here we studied whether HLA-B27 modulates the phosphorylation of signal transducer and activator of transcription 1 (STAT-1 serine 727 residue and the localization of STAT-1 in Salmonella-infected human monocytic cells. In addition, we studied the role of signaling molecule double-stranded RNA activated protein kinase (PKR in these modulatory effects. U937 human monocytic cell transfectants stably expressing wild type HLA-B27 or mutated HLA-B27 heavy chains with amino acid substitutions in the B pocket were prepared. The PMA-differentiated cells were infected with S. enteritidis. Western blotting was used to detect the phosphorylation of STAT-1, and to visualize the localization of STAT-1 in the cells confocal microscopy was used. Specific inhibitors were employed to study the role of PKR in STAT-1 phosphorylation. We discovered that the phosphorylation of STAT-1 serine 727 is prolonged in cells expressing misfolding forms of HLA-B27 after S. enteritidis infection, whereas in mock cells and in cells expressing mutated, non-misfolding HLA-B27 the phosphorylation of serine 727 is transient. Interestingly, STAT-1 serine 727 phosphorylation is partly dependent on PKR. In addition, more STAT-1 is localized in the nucleus of HLA-B27-expressing cells, even before an external trigger, when compared to mock cells. In conclusion, our results show that the phosphorylation of STAT-1 serine 727 residue is prolonged in HLA-B27-expressing monocyte-macrophage U937 cells after bacterial infection. This is of interest since the phosphorylation of serine 727 on STAT-1 is suggested to contribute to macrophage activation and promote inflammatory responses. Therefore, our results provide a mechanism which explains how the expression of an HLA-B

  17. Observer variation in grading sacroiliac radiographs in HLA-B27 positive individuals

    Hollingsworth, P.N.; Cheah, P.S.; Dawkins, R.L.; Owen, E.T.; Calin, A.; Wood, P.H.N. (Royal Perth Hospital (Australia))

    1983-04-01

    This study attempts to reconcile the apparent differences in the reported frequency of ankylosing spondylitis and radiological sacroilitis in HLA-B27 positive individuals. Pelvic radiographs from 125 Busselton subjects were mixed with 81 other films selected to illustrate the possible range of sacroiliac changes and were graded by observers who were involved in 2 of the conflicting studies and by a 3rd independent observer. Concordance was high for advanced bilateral disease but not for unilateral and milder changes. Variation between observers and the interpretation of sacroiliac radiographs is sufficiently large to account for much of the disagreement between frequency estimates.

  18. HLA-B27 associated reactive spondyloarthropathies in a Dutch military hospital.

    Lionarons, R J; van Zoeren, M; Verhagen, J N; Lammers, H A

    1986-01-01

    Forty-two male patients with reactive spondyloarthropathies in a Dutch military hospital are described with mean age of onset 21.9 years. Peripheral arthritis or sacroiliitis was present in all, eye symptoms in 21 (50%), and genitourinary disease in 15 (35.7%). Evidence of antecedent sexually acquired or enterocolitic infection was found only in three (7.2%). HLA-B27 antigen was detected in 34 (81%) of 42 patients. Additional data suggest that reactive spondyloarthropathies are the most commo...

  19. HLA-B27在强直性脊柱炎诊断中的应用

    张红旗; 龙永梅; 卢晓卉

    2008-01-01

    目的:探讨人类白细胞抗原B27(HLA-B27)检测诊断强直性脊柱炎(AS)的意义.方法:应用微量细胞毒法,对441例AS患者、1167例类风湿性关节炎(RA)患者及100例健康体检者进行HLA-B27对比检测.结果:AS组HLA-B27阳性率为91.6%,RA组阳性率为9.3%,健康体检组阳性率为4.0%,AS组明显高于RA组和体检组,差异有统计学意义(X2=1058.74,P<0.005).男性HLA-B27阳性率(42.4%)明显高于女性(17.8%),差异有统计学意义(X2=63.46,P<0.005).不同年龄组 HLA-B27阳性率有明显差异,30岁以下年龄组明显高于其它年龄组.结论:HLA-B27与AS具有很强的相关性,检测HLA-B27对AS的早期诊断和鉴别具有重要意义.

  20. 外周血白细胞HLA-B27的表达及临床意义

    陈军浩; 张葵

    2001-01-01

    目的观察非强直性脊椎炎与强直性脊椎炎(AS)病人外周血白细胞HLA-B27的表达,探讨各类白细胞HLA-B27表达对AS诊断价值.方法用流式细胞术检测了20例AS和32例非AS患者外周血白细胞HLA-B27的表达.结果对照组各群细胞HLA-B27表达量(以平均荧光强度表示)依次为单核细胞(65.33)、T淋巴细胞(52.55)、淋巴细胞(49.46)和粒细胞(17.02).AS组各群细胞HLA-B27表达均显著高于对照组(P<0.01),依次为单核细胞(337.51)、淋巴细胞(227.38)、T淋巴细胞(268.08)和粒细胞(98.47).对照组与AS组淋巴细胞和T淋巴细胞HLA-B27的的表达未见交叉.结论检测淋巴细胞和T淋巴细胞HLA-B27的表达能最好地区别AS与非AS患者.

  1. Altered regulation of ELAVL1/HuR in HLA-B27-expressing U937 monocytic cells.

    Anna S Sahlberg

    Full Text Available OBJECTIVE: To investigate the role of HLA-B27 expression in the regulation of RNA binding protein (RBP Embryonic Lethal Abnormal Vision (ELAV L1/Human antigen R (HuR expression in Salmonella-infected or LPS-stimulated human monocytic cells, since HuR is a critical regulator of the post-transcriptional fate of many genes (e.g. TNFα important in inflammatory response. METHODS: U937 monocytic cells were stably transfected with pSV2neo resistant vector (mock, wild type HLA-B27, or mutated HLA-B27 with amino acid substitutions in the B pocket. Cells were differentiated, infected with Salmonella enteritidis or stimulated with lipopolysaccharide. The expression levels of HuR protein and cleavage products (CP1 and CP2 were detected by Western blotting and flow cytometry. Specific inhibitors were used to study the role of PKR and p38 in HuR expression and generation of CPs. TNFα and IL-10 secretion after p38 and PKR inhibition were measured by ELISA. RESULTS: Full length HuR is overexpressed and HuR cleavage is disturbed in U937 monocytic cells expressing HLA-B27 heavy chains (HC. Increased full length HuR expression, disturbed cleavage and reduced dependence on PKR after infection correlate with the expression of glutamic acid 45 in the B pocket that is linked to the misfolding of HLA-B27. CONCLUSION: Results show that the expression of HLA-B27 HCs modulates the intracellular environment of U937 monocyte/macrophages by altering HuR regulation. This phenomenon is at least partly dependent on the misfolding feature of the B27 molecule. Since HuR is an important regulator of multiple genes involved in inflammatory response observations offer an explanation how HLA-B27 may modulate inflammatory response.

  2. The application value of HLA-B27 detection in the diagnosis of spondyloarthritis%HLA-B27检测在脊柱关节炎诊断中的应用价值

    姚玮

    2014-01-01

    Objective To analyze human leucocyte antigen B27(HLA-B27) diagnostic value in spondyloarthritis(SpA) .Methods Using flow cytometry to detect HLA-B27 expressions of 298 SpA patients(SpA group) and 195 non-SpA patients(non-SpA group) .Results 227 patients were positive in the 493 patients .In SpA group ,the positive rates of HLA-B27 expressing in ankylo-sing spondylitis (AS ) ,juvenile spondyloarthritis (JSpA ) ,reactive arthritis (ReA ) ,undifferentiated spondyloarthritis (USpA ) , psoriatic arthritis(PsA) were 87 .66% ,59 .09% ,58 .33% ,37 .18% ,25 .00% ,respectively .In non-SpA group ,the positive rates of HLA-B27 expressing in rheumatoid arthritis ,rheumatic arthritis ,cervical or lumbar disease ,osteoarthritis ,gouty arthritis were 18 . 18% ,17 .86% ,16 .67% ,11 .76% ,0 .00% ,respectively .The SpA group and the non-SpA group of HLA-B27 positive comparative differences was statistically significant(P<0 .01) .Conclusion The HLA-B27 is correlated with SpA ,and can provide reference for clinical diagnosis of SpA especially the AS .%目的:分析人类白细胞抗原B27(HLA-B27)在脊柱关节炎(SpA)中的诊断价值。方法采用流式细胞术检测298例SpA患者(SpA组)和195例非SpA患者(非SpA组)HLA-B27的表达水平。结果493例患者中,HLA-B27阳性227例。SpA组中强直性脊柱炎(AS)、幼年脊柱关节炎(JSpA)、反应性关节炎(ReA)、未分化型脊柱关节炎(USpA)和银屑病关节炎(PsA)患者的HLA-B27阳性率,分别为87.66%、59.09%、58.33%、37.18%、25.00%;非SpA组中类风湿关节炎、风湿性关节炎、颈椎或腰椎疾病、骨关节炎、痛风性关节炎患者的HLA-B27阳性率,分别为18.18%、17.86%、16.67%、11.76%、0.00%。SpA组与非SpA组HLA-B27阳性率比较差异有统计学意义(P<0.01)。结论HLA-B27与SpA密切相关,可为临床诊断SpA,尤其是AS提供参考。

  3. Intravitreal Triamcinolone Acetonide for Macular Edema in HLA-B27 Negative Ankylosing Spondylitis

    M.M. Moschos

    2010-12-01

    Full Text Available We report a case of a human leukocyte antigen B27 (HLA-B27-negative patient with cystoid macular edema (CME and ankylosing spondylitis (AS after treatment with triamcinolone acetonide. The patient complained of deterioration of visual acuity of the right eye during the last 10 days. At presentation visual acuity of the right eye was 0.2, and the ophthalmic examination did not reveal any sign of active uveitis. Fluorescein angiography (FA and ocular coherent tomography (OCT showed CME. The left eye was normal with a visual acuity of 0.9. Eight weeks after intravitreal injection of triamcinolone acetonide, visual acuity improved to 0.8 and OCT revealed regression of macular edema. Six months later no recurrence was observed. Our case report indicates for the first time that CME may occur in AS independently of the presence of HLA-B27 and intraocular inflammation. Intravitreal use of triamcinolone acetonide can reduce macular edema and restore visual acuity.

  4. A common minimal motif for the ligands of HLA-B*27 class I molecules.

    Alejandro Barriga

    Full Text Available CD8(+ T cells identify and kill infected cells through the specific recognition of short viral antigens bound to human major histocompatibility complex (HLA class I molecules. The colossal number of polymorphisms in HLA molecules makes it essential to characterize the antigen-presenting properties common to large HLA families or supertypes. In this context, the HLA-B*27 family comprising at least 100 different alleles, some of them widely distributed in the human population, is involved in the cellular immune response against pathogens and also associated to autoimmune spondyloarthritis being thus a relevant target of study. To this end, HLA binding assays performed using nine HLA-B*2705-restricted ligands endogenously processed and presented in virus-infected cells revealed a common minimal peptide motif for efficient binding to the HLA-B*27 family. The motif was independently confirmed using four unrelated peptides. This experimental approach, which could be easily transferred to other HLA class I families and supertypes, has implications for the validation of new bioinformatics tools in the functional clustering of HLA molecules, for the identification of antiviral cytotoxic T lymphocyte responses, and for future vaccine development.

  5. A survey to the early and mid-AS patients negative in HLA-B27%HLA-B27表达阴性的早中期强直性脊柱炎患者调查

    刘波; 邱萌

    2011-01-01

    目的:对4年多在我院门诊和病房确诊的早中期强直性脊柱炎(AS)患者进行HLA-B27检测的结果做了对比分析,以了解本地HLA-B27表达阴性与表达阳性的确诊早中期AS患者之间有何差异.方法:采用流式细胞术检测确诊的298例早中期AS患者的HLA-27抗原.研究样本来源于2006年9月~2011年2月在我院临床诊断为早中期AS的患者.结果:临床确诊的早中期AS患者中,HLA-B27表达阴性患者.男女比例约为3.3:1:HLA-B27表达阳性患者,男女比例约为2.9:1,HLA-B27阴性患者与同性别阳性患者平均年龄比较,差异无统计学意义(P>0.05).临床确诊的早中期AS患者中,HLA-B27表达阴性患者各年龄段的阴性率比较,差异无统计学意义(P>0.05),HLA-B27表达阳性患者各年龄段的阳性率比较,差异无统计学意义(P>0.05).结论:本地HLA-B27表达阴性与表达阳性的确诊早中期AS患者之间,男女比例、平均年龄、HLA-B27阳性率和阴性率无显著差异.在临床诊断时,对HLA-B27表达阴性的疑似患者也要给予同样的重视,以免漏诊和误诊.%Objective: To detect HLA-B27 with early and mid ankylosing spondylitis (AS) patients, who were diagnosed during more than four years, then these two results were compared. To find out the difference between HLA-B27 negative and positive expression of AS in patients diagnosed early and mid AS. Methods: Flow cytometry was used to measure HLA-27 antigen in 298 patients diagnosed as early and mid AS. The patients who were diagnosed of early and mid-AS in our hospital from the September 2006 to February 2011 were regarded as study sample. Results: In clinically diagnosed patients with early and mid-AS, the ratio of males to females with HLA-B27 negative expression was about 3.3:1; the ratio of males to females with HLA-B27 positive expression was about 2.9∶1, there were no significant difference in mean age between two groups of HLA-B27 negative patients with the same sex

  6. Characterization of the Recognition Specificity of BH2, a Monoclonal Antibody Prepared against the HLA-B27 Heavy Chain

    Hui-Chun Yu

    2015-04-01

    Full Text Available BH2, a monoclonal antibody prepared against the denatured human leukocytic antigen-B27 heavy chain (HLA-B27 HC, can immunoprecipitate the misfolded HLA-B27 HC complexed with Bip in the endoplasmic reticulum and recognize the homodimerized HLA-B27 HC that is often observed on the cell membrane of patients suffered from ankylosing spondylitis (AS. However, the recognition specificity of BH2 toward the other molecules of HLA-B type and toward the different types of HLA molecules remained uncharacterized. In this study, we carried out the HLA-typing by using the Luminex Technology to characterize the recognition specificity of BH2 and analyzed the binding domain of HLA-B27 HC by BH2. Our results indicated that BH2 preferably binds to molecules of HLA-B and -C rather than HLA-A and the binding site is located within the α2 domain of HLA-B27 HC.

  7. 广西壮族强直性脊柱炎患者HLA-B27亚型的研究

    陈志坚; 李山

    2007-01-01

    目的:对广西壮族强直性脊柱炎(AS)患者进行HLA-B27亚型检测并探讨其临床意义.方法:采用聚合酶链反应-序列特异性引物(PCR-SSP)法对广西壮族65例AS患者和260例健康体检者的HLA-B27进行检测,并对HLA-B27阳性的标本进行亚型分型.结果:AS组的HLA-B27阳性率明显高于对照组(P0.05).结论:广西壮族AS与HLA-B27存在相关性;AS与HLA-B27各亚型的特异基因可能无关.

  8. Determination of HLA-B27 subtypes in patients with ankylosing spondylitis by PCR-SSP%采用PCR-SSP方法检测强直性脊柱炎患者HLA-B27亚型

    韩军艳; 熊平; 严鹏; 邵诗颖; 熊蓓; 吴雄文; 龚非力

    2003-01-01

    目的建立序列特异性引物-聚合酶链方法(PCR-SSP)检测湖北地区人白细胞抗原B27(Human leucocyte antigen-B27, HLA-B27)阳性强直性脊柱炎患者的HLA-B27亚型组成.方法盐析法提取100例HLA-B27阳性的强直性脊柱炎患者外周血基因组DNA,采用PCR-SSP技术分别进行HLA-B位点基因型检测和HLA-B27基因亚型分析.结果 100例标本PCR-SSP扩增均获成功,历时3 h.共检出4种亚型:HLA-B*2704、HLA-B*2711、HLA-B*2715、HLA-B*2722.其中HLA-B*2704纯合子25例、HLA-B*2704杂合子63例;HLA-B*2711杂合子5例;HLA-B*2715杂合子6例;HLA-B*2722杂合子1例.四种亚型所占构成比分别为90.4%、4%、4.8%和0.8%.结论 PCR-SSP方法进行HLA-B27亚型分析,快速、经济、简单;HLA-B*2704是湖北地区强直性脊柱炎患者HLA-B27基因主要亚型.

  9. Rapid Antigen Processing and Presentation of a Protective and Immunodominant HLA-B*27-restricted Hepatitis C Virus-specific CD8+ T-cell Epitope

    Julia Schmidt; Iversen, Astrid K N; Stefan Tenzer; Emma Gostick; Price, David A.; Volker Lohmann; Ute Distler; Paul Bowness; Hansjörg Schild; Blum, Hubert E.; Paul Klenerman; Christoph Neumann-Haefelin; Robert Thimme

    2012-01-01

    HLA-B*27 exerts protective effects in hepatitis C virus (HCV) and human immunodeficiency virus (HIV) infections. While the immunological and virological features of HLA-B*27-mediated protection are not fully understood, there is growing evidence that the presentation of specific immunodominant HLA-B*27-restricted CD8+ T-cell epitopes contributes to this phenomenon in both infections. Indeed, protection can be linked to single immunodominant CD8+ T-cell epitopes and functional constraints on e...

  10. Two-dimensional gel analysis demonstrates no structural alteration of HLA-B27 polypeptides between patients with ankylosing spondylitis and healthy individuals.

    Trapani, J A; Walker, I D; McKenzie, I. F.

    1984-01-01

    After precipitation of the HLA-B27 antigen from the surface of peripheral blood lymphocytes (PBL) by means of an anti-HLA-B27 allospecific monoclonal antibody 2-dimensional gel electrophoresis was used to compare the structure of the B27 antigens derived from 5 patients with ankylosing spondylitis with that of healthy HLA-B27 positive counterparts. No significant difference in polypeptide structure was noted, which suggests that the pathogenesis of ankylosing spondylitis does not involve a st...

  11. Erregungsbildung und Erregungsleitung des Herzens bei HLA B27-assozierter juveniler Arthritis in Ruhe und unter körperlicher Belastung

    Sachs, Anke

    2005-01-01

    Eine Herzbeteiligung in Form von Reizleitungsstörungen, Klappenfunktionsstörungen und Funktionsstörungen des Myokards bei erwachsenen Patienten mit HLA B27-positver Spondyloarthropathien ist seit langem bekannt. Überlegungen existieren, dass diese kardialen Veränderungen eher mit der HLA B27-Eigenschaft anstatt mit der Arthritis in Zusammenhang stehen. Der Zeitpunkt, an dem gerade die Reizbildungs- und Reizleitungsstörungen beginnen, ist unklar. Daher verglichen wir in unserer Studie HLA B27-...

  12. Endogenous Processing and Presentation of T-cell Epitopes from Chlamydia trachomatis with Relevance in HLA-B27-associated Reactive Arthritis*

    Cragnolini, Juan J.; García-Medel, Noel; López de Castro, José A.

    2009-01-01

    Chlamydia trachomatis triggers reactive arthritis, a spondyloarthropathy linked to the human major histocompatibility complex molecule HLA-B27, through an unknown mechanism that might involve molecular mimicry between chlamydial and self-derived HLA-B27 ligands. Chlamydia-specific CD8+ T-cells are found in reactive arthritis patients, but the immunogenic epitopes are unknown. A previous screening of the chlamydial genome for putative HLA-B27 ligands predicted multiple peptides that were recog...

  13. Usage of Conventional PCR Technology for the Detection of HLA-B27 Allele: A Significant Molecular Marker of Ankylosing Spondylitis

    Sharma, Narotam; Sharma, Veena; Masood, Tariq; Nautiyal, Satish Chandra; Sailwal, Shivani; Singh, Rajesh K.; Kushwaha, Rajeev K.; R. K. Singh

    2012-01-01

    Ankylosing spondylitis is a chronic inflammatory disease that has been linked to the human leukocyte antigen class I allele HLA-B27. More than 90 % of patients with ankylosing spondylitis possess the HLA-B27 allele, but only 1 % of people with HLA-B27 develop the disease. Ankylosing spondylitis predominately affects young males. The present study was planned to find out the involvement of HLA-B27 specific allele in relation to age and sex in symptomatic suspected patients of ankylosing spondy...

  14. Low T cell production of TNFα and IFNγ in ankylosing spondylitis: its relation to HLA-B27 and influence of the TNF-308 gene polymorphism

    Rudwaleit, M; Siegert, S.; Yin, Z; Eick, J; Thiel, A.; Radbruch, A; Sieper, J; Braun, J.

    2001-01-01

    OBJECTIVE—To test the hypothesis that ankylosing spondylitis (AS) is a T helper cell type 2 polarised disease by quantifying the T cell cytokines interferon γ (IFNγ), interleukin 4 (IL4), tumour necrosis factor α (TNFα), and IL10 at the single cell level in patients with AS in comparison with healthy HLA-B27 negative and HLA-B27 positive controls.
METHODS—Peripheral blood mononuclear cells from 65 subjects (25 HLA-B27 positive patients with active AS, 18 healthy HLA-B27 positive controls, and...

  15. Serum antibodies from patients with ankylosing spondylitis and Reiter's syndrome are reactive with HLA-B27 cells transfected with the Mycobacterium tuberculosis hsp60 gene.

    Kellner, H; Wen, J.; Wang, J; Raybourne, R B; Williams, K. M.; Yu, D T

    1994-01-01

    HLA-B27-related arthritis is probably mediated by an immune response against HLA-B27 complexed with peptides derived from proteins of arthritis-causing bacteria. Immunogenic proteins with a high degree of homology among bacteria, such as in the hsp60 family, are likely candidates. To create such complexes experimentally, we transfected an HLA-B27 cell line with the Mycobacterium tuberculosis hsp60 gene. Because of previous observations that HLA-B27-peptide complexes can be distinguished by an...

  16. HLA-A*01:03, HLA-A*24:02, HLA-B*08:01, HLA-B*27:05, HLA-B*35:01, HLA-B*44:02, and HLA-C*07:01 Monochain Transgenic/H-2 Class I Null Mice

    Boucherma, Rachid; Kridane-Miledi, Hédia; Bouziat, Romain;

    2013-01-01

    We have generated a panel of transgenic mice expressing HLA-A*01:03, -A*24:02, -B*08:01, -B*27:05, -B*35:01, -B*44:02, or -C*07:01 as chimeric monochain molecules (i.e., appropriate HLA α1α2 H chain domains fused with a mouse α3 domain and covalently linked to human β2-microglobulin). Whereas...... surface expression of several transgenes was markedly reduced in recipient mice that coexpressed endogenous H-2 class I molecules, substantial surface expression of all human transgenes was observed in mice lacking H-2 class I molecules. In these HLA monochain transgenic/H-2 class I null mice, we observed...... a quantitative and qualitative restoration of the peripheral CD8(+) T cell repertoire, which exhibited a TCR diversity comparable with C57BL/6 WT mice. Potent epitope-specific, HLA-restricted, IFN-γ-producing CD8(+) T cell responses were generated against known reference T cell epitopes after either...

  17. HLA-B27-ASSOCIATED UVEITIS: EPIDEMIOLOGY, CLINICAL PICTURE, AND COMPLICATIONS

    T. V. Dubinina

    2014-01-01

    Full Text Available Anterior uveitis is the most common form of intraocular inflammation. Among them, HLA-B27-associated uveitis occupies one of the leading places, which may be an independent disease or one of the manifestations of spondy- loarthritis (SA. The paper considers the general issues of the nomenclature and classification of uveitis, by using the classification criteria of the International Uveitis Study Group and the Standardization of Uveitis Nomenclature Workshop. The epidemiological aspects of uveitis are described. Emphasis is laid on a difference in the detection rate of uveitis in different countries, in men and women, as well as in different forms of SA. The clinical features of SA- associated uveitis and its complications are discussed. 

  18. 强直性脊柱炎与HLA-B27基因亚型动态定量表达的相关性研究*

    黄建民; 李荣需; 周玲; 陈冬萍; 刘永青; 林周胜

    2013-01-01

    目的:探讨不同HLA-B27基因亚型与强直性脊柱炎的相关性及其动态表达量与强直性脊柱炎疾病活动指数(BASDAI)的关系。方法:收集骨科门诊强直性脊柱炎患者应用流式细胞术定性检测HLA-B27阳性100例,HLA-B27阳性健康对照组30例,通过PCR-SSP(序列引物扩增法)技术检测HLA-B27基因亚型;荧光实时定量RT-PCR技术检测HLA-B27阳性AS患者及HLA-B27阳性健康对照组HLA-B27亚型的mRNA,统计分析两组HLA-B27mRNA表达量及HLA-B27阳性患者HLA-B27mRNA表达水平与强直性脊柱炎患者疾病活动指数的相关性。结果:100例AS患者检出HLA-B2704亚型55例,HLA-B2705亚型45例,30对照组检出HLA-B2704亚型13例, HLA-B2705亚型12例,HLA-B2706亚型3例,HLA-B2709亚型2例;HLA-B27阳性患者HLA-B27mRNA表达水平最低值为1.16,最高值为37.01,均值7.77,HLA-B27阳性健康对照组表达水平最低1.05,最高值2.10,均值为1.51,两组差异有统计学意义(P=0.002);HLA-B27阳性患者HLA-B27mRNA表达水平与强直性脊柱炎患者疾病活动指数密切相关(R=0.707)。结论:本地区AS患者HLA-B27基因亚型以B2704和B2705为主,B2706及B2709可能为保护亚型;HLA-B27阳性患者HLA-B27mRNA 表达水平高于HLA-B27阳性健康者;HLA-B27阳性患者HLA-B27mRNA表达水平与强直性脊柱炎患者疾病活动指数呈正相关。

  19. Körperliche Leistungsfähigkeit bei Patienten mit HLA B27 positiver juveniler idiopathischer Arthritis in Remission

    Fischer, Michael Johannes

    2013-01-01

    Mit dieser Arbeit sollte untersucht werden, ob es eine Beeinträchtigung der körperlichen Leistungsfähigkeit bei Patienten bis zum 20. Lebensjahr mit inaktiver juveniler idiopathischer Arthritis bzw. einer Arthritis in Remission im Vergleich zu gesunden Gleichaltrigen gibt und wenn ja, ob ein Zusammenhang zu dem Eiweißkörper HLA B27 besteht.

  20. HLA-B27 predicts a more extended disease with increasing age at onset in boys with juvenile idiopathic arthritis

    Berntson, Lillemor; Damgård, Michael; Andersson-Gäre, Boel;

    2008-01-01

    OBJECTIVE: Juvenile idiopathic arthritis (JIA) is a heterogeneous condition with very few clinical and laboratory signs that can help predict the course and severity of the disease in the individual patient. The cell-surface antigen HLA-B27 is well known to be associated with spondyloarthropathies...... population-based study as possible. METHODS: We studied an incidence-based cohort of 305 patients collected prospectively in 3 Nordic countries (Sweden, Norway, Denmark). Clinical and serological data of the first 3 years of the disease were collected. RESULTS: HLA-B27 was found to be positive in 25.5% of...... the patients, and we found a higher proportion of HLA-B27-positive boys with older age at disease onset (p=0.034). Regression analysis showed a correlation of 0.7 in the HLA-B27-positive boys, pointing to a higher risk of more joint involvement with older age at disease onset. By Fisher's exact test...

  1. HLA-B27抗原関連急性前部ぶどう膜炎の臨床像についての検討

    菊池, 三季; KIKUCHI, Miki

    1997-01-01

    An objective investigation of the clinical features of HLA-B27-associated acute anterior uveitis (HLA-B27 AAU) was performed using a laser flare cell meter, and by laboratory testing of peripheral blood. The study involved 42 patients (31 men and 11 women, mean age at onset 31.3 years) with HLA-B27 AAU, 33 patients with HLA-B27 negative AAU, and 40 patients with Behcet's disease. The flare intensity was significantly higher at their first attack in HLA-B27(+) patients than in HLA-B27(-) patie...

  2. 强直性脊柱炎疑似患者HLA-B27检测结果分析

    章涛; 方宁; 祁莹; 陈代雄

    2008-01-01

    对111例疑似强直性脊柱炎(AS)患者外周血T淋巴细胞HLA-B27表达进行检测,结合临床资料对AS作出诊断.共检测出HLA-B27阳性49例,总检出率为44%,结合临床资料确诊AS患者46例,包括男性HLA-B27+As患者40例、HLA-B27-AS 3例,女性HLA-B27+AS 2例、HLA-B27-AS 1例,男女患病比为4.4∶1.男性AS确诊患者的HLA-B27阳性率为88%(40/43).提示HLA-B27检测有助于疑似AS病例的确诊.

  3. 465例强直性脊柱炎患者HLA-B27结果的调查分析

    魏云玉; 黄红宇; 陆红兵; 李娟; 马宁

    2008-01-01

    目的 通过对465例强直性脊柱炎患者外周血HLA-B27的检测,了解HLA-B27阴阳性患者在强直性脊柱炎中的情况.方法 用流式细胞术对强直性脊柱炎患者的HLA-B27进行测定.结果 HLA-B27阴性患者占10.5%,HLA-B27阳性患者占89.5%.结论 HLA-B27是诊断强直性脊柱炎的一个重要指标,但不能单独从HLA-B27的阴阳性来判断强直性脊柱炎.还需要结合临床进行综合判断.另外我们还发现HLA-B27阴性强直性脊柱炎患者女性比例较高.

  4. Characterization of a Proteasome and TAP-independent Presentation of Intracellular Epitopes by HLA-B27 Molecules

    Magnacca, A.

    2012-07-17

    Nascent HLA-class I molecules are stabilized by proteasome-derived peptides in the ER and the new complexes proceed to the cell surface through the post-ER vesicles. It has been shown, however, that less stable complexes can exchange peptides in the Trans Golgi Network (TGN). HLA-B27 are the most studied HLA-class I molecules due to their association with Ankylosing Spondylitis (AS). Chimeric proteins driven by TAT of HIV have been exploited by us to deliver viral epitopes, whose cross-presentation by the HLA-B27 molecules was proteasome and TAP-independent and not restricted to Antigen-Presenting Cells (APC). Here, using these chimeric proteins as epitope suppliers, we compared with each other and with the HLA-A2 molecules, the two HLA-B*2705 and B*2709 alleles differing at residue 116 (D116H) and differentially associated with AS. We found that the antigen presentation by the two HLA-B27 molecules was proteasome-, TAP-, and APC-independent whereas the presentation by the HLA-A2 molecules required proteasome, TAP and professional APC. Assuming that such difference could be due to the unpaired, highly reactive Cys-67 distinguishing the HLA-B27 molecules, C67S mutants in HLA-B*2705 and B*2709 and V67C mutant in HLA-A*0201 were also analyzed. The results showed that this mutation did not influence the HLA-A2-restricted antigen presentation while it drastically affected the HLA-B27-restricted presentation with, however, remarkable differences between B*2705 and B*2709. The data, together with the occurrence on the cell surface of unfolded molecules in the case of C67S-B*2705 mutant but not in that of C67S-B*2709 mutant, indicates that Cys-67 has a more critical role in stabilizing the B*2705 rather than the B*2709 complexes.

  5. HLA-B27检测在豫西地区强直性脊柱炎诊断中的意义

    刘好

    2006-01-01

    用特异性人类白细胞抗原(HLA)-B27抗血清对来自豫西地区临床诊断为强直性脊柱炎(AS)的300例患者进行HLA-B27检测.结果HLA-B27阳性率为91%(男性患者为97%,女性为85%).认为AS与HLA-B27相关性强,HLA-B27血清学检测对豫西地区AS患者的诊断和鉴别诊断具有重要意义.

  6. HLA-B27检测在早期脊柱关节病中的诊断作用

    邵标; 王辉

    2008-01-01

    目的 了解HLA-B27在早期脊柱关节病中的诊断作用.方法 采用单克隆抗体血清法对245例早期脊柱关节病人及89例健康者进行HLA-B27检测.结果 245例早期脊柱关节病痛人组中,HLA-B27阳性47例,阳性率为19.18%;其中男性34例,女性13例;89例正常对照组3例HLA-B27阳性,阳性率为3.37%.结论 HLA-B27对早期脊柱关节病患者具有诊断和鉴别诊断作用.

  7. HLA-B27检测在强直性脊柱炎诊断中的临床价值探讨

    刘晖

    2010-01-01

    目的 探讨HLA-B27检测在强直性脊柱炎(AS)诊断中的临床价值.方法 选择我院确诊的AS患者83例作为观察组,随机抽取在我院健康体检人群80例做为对照组,进行HLA-B27检测.结果 观察组HLA-B27阳性率明显高于健康对照组,差异有统计学意义(P<0.01).结论 HLA-B27与AS具有很强的相关性,检测HLA-B27可明显提高诊断特异性.

  8. An HLA-B27 Homodimer Specific Antibody Recognizes a Discontinuous Mixed-Disulfide Epitope as Identified by Affinity-Mass Spectrometry

    Iuraşcu, Marius-Ionuţ; Marroquin Belaunzanar, Osiris; Cozma, Claudia; Petrausch, Ulf; Renner, Christoph; Przybylski, Michael

    2016-06-01

    HLA-B27 homodimer formation is believed to be a hallmark of HLA-B27 associated spondyloarthritides. Recently, we have generated a homodimer-specific monoclonal antibody (HD6) and have demonstrated that HLA-B27 homodimer complexes are present on monocytes of healthy HLA-B27 gene carriers at low levels, with significantly increased levels at active disease. The capability of the HD6 antibody to discriminate between correctly formed HLA-B27 heterotrimers and pathology-associated homodimers is striking and cannot be explained by the primary structure of HLA-B27. We hypothesized that HD6 accesses a unique epitope and used affinity-mass spectrometry for its identification. The HD6 antibody was immobilized on an activated sepharose affinity column, and HLA-B27 homodimer characterized for affinity. The epitope was identified by proteolytic epitope excision and MALDI mass spectrometry, and shown to comprise a discontinuous Cys-203- 257-Cys mixed-disulfide peptide structure that is not accessible in HLA-B27 heterotrimers due to protection by noncovalently linked β2-microglobulin. The epitope peptides were synthesized by solid phase peptide synthesis, and the two monomeric peptide components, HLA-B27(203-219) and HLA-B27(257-273), as well as the homo- and hetero-dimeric disulfide linked combinations prepared. The affinity binding constants KD towards the antibodies were determined using a surface acoustic wave (SAW) biosensor, and showed the highest affinity with a KD of approximately 40 nM to the HD6 antibody for the (203-219)-SS-(257-273) mixed disulfide epitope.

  9. An HLA-B27 Homodimer Specific Antibody Recognizes a Discontinuous Mixed-Disulfide Epitope as Identified by Affinity-Mass Spectrometry

    Iuraşcu, Marius-Ionuţ; Marroquin Belaunzanar, Osiris; Cozma, Claudia; Petrausch, Ulf; Renner, Christoph; Przybylski, Michael

    2016-04-01

    HLA-B27 homodimer formation is believed to be a hallmark of HLA-B27 associated spondyloarthritides. Recently, we have generated a homodimer-specific monoclonal antibody (HD6) and have demonstrated that HLA-B27 homodimer complexes are present on monocytes of healthy HLA-B27 gene carriers at low levels, with significantly increased levels at active disease. The capability of the HD6 antibody to discriminate between correctly formed HLA-B27 heterotrimers and pathology-associated homodimers is striking and cannot be explained by the primary structure of HLA-B27. We hypothesized that HD6 accesses a unique epitope and used affinity-mass spectrometry for its identification. The HD6 antibody was immobilized on an activated sepharose affinity column, and HLA-B27 homodimer characterized for affinity. The epitope was identified by proteolytic epitope excision and MALDI mass spectrometry, and shown to comprise a discontinuous Cys-203- 257-Cys mixed-disulfide peptide structure that is not accessible in HLA-B27 heterotrimers due to protection by noncovalently linked β2-microglobulin. The epitope peptides were synthesized by solid phase peptide synthesis, and the two monomeric peptide components, HLA-B27(203-219) and HLA-B27(257-273), as well as the homo- and hetero-dimeric disulfide linked combinations prepared. The affinity binding constants KD towards the antibodies were determined using a surface acoustic wave (SAW) biosensor, and showed the highest affinity with a KD of approximately 40 nM to the HD6 antibody for the (203-219)-SS-(257-273) mixed disulfide epitope.

  10. Significance of the Expression Quantity of HLA-B27 Gene and Its Subtype in Estimating Patients with Ankylosing Spondylitis%HLA-B27基因表达量及亚型分析在 AS 疾病评估中的意义

    孙灵迪; 王平均; 鹿永; 武晓茜; 邵先安

    2016-01-01

    Objective To investigate whether expression quantity of HLA-B27 and its subtypes associated with incidence of AS,the gene expression of HLA-B27 and its subtypes were detected in patients with AS.Methods 120 cases patients sus-pected with AS and 50 healthy subjects were enrolled in the study.Main demographic and clinical characteristics of the sub-jects were collected.Meanwhile total RNA was isolated from peripheral blood and real time RT-PCR was used to measure the quantitative expression of HLA-B27 gene.Besides RT-PCR,sequence-based typing (SBT)method was used to confirm the HLA-B27 subtype.All experimental data were analyzed by SPSS17.0 software and P values <0.05 were considered to be significant.Results Firstly,there were 55 subjects were finally diagnosed as AS patients among the 120 patients suspec-ted with AS.There were 53 subjects whose HLA-B27 was positive (96.36%)in 55 patients with AS.It showed that there was a correlation between BASDAI and expression quantity of HLA-B27 gene (r=0.845,P =0.000).Five subtypes were found and which were HLA-B27∶04 subtype (29/53,54.72%),HLA-B27:05 subtype (20/53,37.74%),HLA-B27 ∶02 subtype (2/53,3.77%),HLA-B27∶03 subtype (1/53,1.89%)and HLA-B27∶07 subtype (1/53,1.89%),respectively. Among the 50 healthy subjects,there were only one kind of subtype (2/50,4%),which was HLA-B27∶04.There were no statistical difference in the age (t=0.711,P =0.480),sex (χ2 =0.880,P =0.348),family history (χ2 =0.011,P =0.916) and treatment (χ2 =0.113,P =0.736)between the HLA-B27∶04 and HLA-B27∶05 subtypes.Conclusion HLA-B27∶04 and HLA-B27∶05 were primary subtypes in AS patients which HLA-B27 positive.There was a correlation between gene expression quantity of HLA-B27 and AS disease activity index.%目的:探讨人类白细胞抗原(human leukocyto antigens,HLA)B27(HLA-B27)基因表达量及其亚型在强直性脊柱炎疾病诊疗中的意义。方法收集120例疑似强直性脊柱炎患者和50例健康体检

  11. Relationship between Ankylosing Spondylitis and HLA-B27,HLA-Cw and KIRs%HLA-B27、HLA-Cw、KIRs与强直性脊柱炎

    张谷香

    2012-01-01

    强直性脊柱炎(AS)是一种慢性进行性炎症性关节炎,具有明显的家族倾向.虽然,人类白细胞抗原B27(HLA-B27)被认为与AS的发病密切相关,但是由于AS发病机制复杂,HLA-B27仅为导致AS易感的主要基因之一.近年来研究发现,自然杀伤细胞免疫球蛋白样受体(KIRs)和HLA-Cw基因与AS的发生发展也有一定的相关性,且HLA-Cw与活化性/抑制性KIRs之间的失衡可能是影响AS发病机制的关键因素.%Ankylosing spondylitis( AS )is a kind of chronic progressive arthritis which presents clear familial predisposition. Although it has been confirmed that the expression of human leukocyte an-tigen-B27( HLA-B27 )closely related to AS,it is not the only susceptibility genes on AS. Recent studies found that killer immunoglobulin-like receptors( KIR )and HLA-Cw also participated the generation and development of AS. The imbalance between HLA-Cw and activation/inhibition of KIRs might play an important role in the pathogenes is of AS.

  12. The Rate of Helicobacter pylori Seropositivity in a Group of Korean Patients with HLA-B27-Associated Acute Anterior Uveitis.

    Jeong Hun Bae

    Full Text Available To investigate an association between Helicobacter pylori seropositivity and HLA-B27-positive acute anterior uveitis (AAU in Korean patients.Retrospective analysis was performed with data from 106 patients previously diagnosed with AAU without clinical evidence of spondyloarthropathy. Serum immunoglobulin G antibodies to H. pylori were measured by enzyme-linked immunosorbent assay, and HLA typing was performed using polymerase chain reaction of DNA amplification. We included 72 non-uveitis patients and 35 age- and sex-matched healthy controls in the study.Of the 106 patients with AAU, 41 (38.7% were HLA-B27-positive, and 45 (42.5% were seropositive for H. pylori. Patients with HLA-B27-positive AAU had a significantly lower prevalence of H. pylori seropositivity compared to those with HLA-B27-negative AAU and healthy controls (24.4% vs. 53.8%, p = 0.003; 24.4% vs. 57.1%, p = 0.004, respectively. In the non-uveitis group, however, HLA-B27-positive patients exhibited similar H. pylori seropositivity prevalence to HLA-B27-negative patients and healthy controls (45.5% vs. 55.7%, p = 0.529; 45.5% vs. 57.1%, p = 0.497, respectively. In multivariate analysis, a low prevalence of H. pylori seropositivity was significantly associated with HLA-B27-positive AAU (odds ratio = 0.340, 95% confidence interval 0.135-0.855, p = 0.022.Our results suggest an inverse association between H. pylori seropositivity and HLA-B27-positive AAU. Further investigation of this association is needed, given the low prevalence of H. pylori seropositivity observed in patients with HLA-B27-positive AAU.

  13. Microarray Analysis of Response of Salmonella during Infection of HLA-B27- Transfected Human Macrophage-Like U937 Cells

    Hinton Jay CD; He Qiushui; Danino Vittoria; Ge Shichao; Granfors Kaisa

    2010-01-01

    Abstract Background Human leukocyte antigen (HLA)-B27 is strongly associated with the development of reactive arthritis (ReA) in humans after salmonellosis. Human monocytic U937 cells transfected with HLA-B27 are less able to eliminate intracellular Salmonella enterica serovar Enteritidis than those transfected with control HLA antigens (e.g. HLA-A2). To investigate further the mechanisms by which HLA-B27-transfected cells allow increased replication of these bacteria, a DNA-based microarray ...

  14. HLA-B27 Modulates Intracellular Growth of Salmonella Pathogenicity Island 2 Mutants and Production of Cytokines in Infected Monocytic U937 Cells

    Shichao Ge; Qiushui He; Kaisa Granfors

    2012-01-01

    BACKGROUND: Salmonella enterica serovar Enteritidis PT4 KS8822/88 replicates rapidly in HLA-B27-transfected human monocytic U937 cells. In this process, Salmonella pathogenicity island 2 (SPI-2) genes play a crucial role. Our previous study indicated that 118 Salmonella genes, including 8 SPI-2 genes were affected by HLA-B27 antigen during Salmonella infection of U937 cells. METHODS/PRINCIPAL FINDINGS: To further investigate Salmonella replication in HLA-B27-positive U937 monocytic cells, two...

  15. Autoantibodies to the HLA-B27 sequence cross-react with the hypothetical peptide from the arthritis-associated Shigella plasmid.

    Tsuchiya, N.; Husby, G; Williams, R. C.; Stieglitz, H; Lipsky, P E; Inman, R.D.

    1990-01-01

    We previously reported elevated serum antibody levels to a peptide representing the HLA-B27 polymorphic region (B27 peptide) in HLA-B27(+) ankylosing spondylitis (AS) patients. A plasmid (pHS-2) isolated from arthritogenic Shigella flexneri strains had been shown to encode an amino acid sequence homologous to HLA-B27. Rabbit antibody to this sequence (pHS-2 peptide) strongly cross-reacted with B27 peptide and, to a much lesser extent, with Klebsiella nitrogenase peptide. Serum antibody levels...

  16. Expression of arthritis-causing HLA-B27 on Hela cells promotes induction of c-fos in response to in vitro invasion by Salmonella typhimurium.

    Ikawa, T; Ikeda, M; Yamaguchi, A; Tsai, W.C.; Tamura, N; Seta, N; Trucksess, M; Raybourne, R B; Yu, D T

    1998-01-01

    HLA-B27 confers a very strong genetic predisposition to development of a reactive arthritis after infection by bacteria such as Salmonella typhimurium. This study examines the role of HLA-B27 in the initiation of the earliest host activities after exposure to Salmonella, namely activation of the immediate early genes in the epithelial cells. Our major finding is that in Hela cells, the expression of c-fos was induced by Salmonella invasion only when the cells expressed the transfected HLA-B27...

  17. Vision-Related Quality of Life in Patients with Inactive HLA-B27-Associated-Spectrum Anterior Uveitis.

    Hoeksema, Lisette; Los, Leonoor I

    2016-01-01

    We investigated the vision-related quality of life (VR-QOL) in patients with HLA-B27 associated anterior uveitis (AU). The study was conducted in 2012 at the ophthalmology department of the University Medical Center of Groningen. We included AU patients who were HLA-B27 positive and/or were diagnosed by a rheumatologist with an HLA-B27 associated systemic disease. Sixty-one of 123 (50%) adult patients participated. All patients filled-out the National Eye Institute Visual Functioning Questionnaire-25 (NEI VFQ-25), Beck Depression Inventory (BDI-II), social support lists and an additional questionnaire for gathering general information. Medical records were reviewed for clinical characteristics. Analyses were conducted on various patient and ocular characteristics. We compared our NEI VFQ-25 scores with those previously found in the literature. Our main outcome measures were VR-QOL scores and their associations with various general patient and ocular characteristics. We found that the NEI VFQ-25 mean overall composite score was 88.9±8.8, which is relatively high, but lower than that found in a normal working population. The mean general health score was 47.4±20.8, which is lower than in patients with other ocular diseases. Patients with a systemic disease scored significantly lower on general health and VR-QOL, compared to patients without a systemic disease. Patients with a depression (6/59 (10%)) frequently had ankylosing spondylitis (5/6 patients) and they scored significantly worse on VR-QOL. We concluded that patients with HLA-B27 associated AU have a relatively high VR-QOL. However, the presence of a systemic disease is associated with lower VR-QOL and general health scores. In addition, depression is associated with a lower VR-QOL. PMID:26808922

  18. Ultrasonographic Assessment of Enthesitis in HLA-B27 Positive Patients with Rheumatoid Arthritis, a Matched Case-Only Study

    Antonio Mera-Varela; Aida Ferreiro-Iglesias; Eva Perez-Pampin; Marisol Porto-Silva; Gómez-Reino, Juan J.; Antonio Gonzalez

    2013-01-01

    INTRODUCTION: HLA-B27 has a modifier effect on the phenotype of multiple diseases, both associated and non-associated with it. Among these effects, an increased frequency of clinical enthesitis in patients with Rheumatoid Arthritis (RA) has been reported but never explored again. We aimed to replicate this study with a sensitive and quantitative assessment of enthesitis by using standardized ultrasonography (US). METHODS: The Madrid Sonography Enthesitis Index (MASEI) was applied to the US as...

  19. Application of a Simple In-House PCR-SSP Technique for HLA-B* 27 Typing in Spondyloarthritis Patients

    Parasannanavar, Devraj J.; Anjali Rajadhyaksha; Kanjaksha Ghosh

    2013-01-01

    Background. Microlymphocytotoxicity (MLCT) and flowcytometry (FC) are the conventional serological methods to detect HLA-B* 27. Due to some disadvantages in these methods, most of the HLA laboratories have now switched over to molecular methods. Molecular techniques based on commercial kits are expensive; as such many laboratories with limited funds in developing countries cannot afford these techniques. Aims. Our main aim was to standardize a simple inexpensive in-house PCR-SSP technique for...

  20. Alteration of HLA-B27 Peptide Presentation after Infection of Transfected Murine L Cells by Shigella flexneri

    Boisgérault, Florence; Mounier, Joëlle; Tieng, Vannary; Stolzenberg, Marie-Claude; Khalil-Daher, Iman; Schmid, Michel; Sansonetti, Philippe; Charron, Dominique; Toubert, Antoine

    1998-01-01

    Shigella flexneri is a triggering agent for reactive arthritis in HLA-B27-susceptible individuals. Considering the intracellular multiplication of bacteria, it seems likely that bacterial peptides may be presented by the major histocompatibility complex (MHC) class I pathway. To examine this hypothesis, we infected HLA-B*2705- and/or human β2-microglobulin-transfected murine L-cell lines with M90T, an invasive strain of S. flexneri. Bacterial infection induced no detectable modifications in t...

  1. Validity of magnetic resonance image and HLA-B27 in early detection of sacroiliitis in Egyptian spondyloarthropathic patients

    El-Shereef, Rawhya R.; Amal Ali; Ayman Darwish; Fatma Ali; Mohammed Ibrahim; Lamia Hamdy

    2015-01-01

    Objective The aim of this study was to compare the validity of MRI in the early detection of sacroiliitis with laboratory findings of human leukocyte antigen-B27 (HLA-B27), conventional radiography, and clinical assessment. Participants and methods Sixty patients with spondyloarthropathy (group II) with duration of illness less than 2 years and 20 healthy controls (group I) were included in this study. Both groups were subjected to assessment of history, clinical examination, and lab...

  2. Structural analysis of an HLA-B27 functional variant, B27d detected in American blacks

    Rojo, S.; Aparicio, P.; Hansen, J.A.; Choo, S.Y.; Lopez de Castro, J.A.

    1987-11-15

    The structure of a new functional variant B27d has been established by comparative peptide mapping and radiochemical sequencing. This analysis complete the structural characterization of the six know histocompatibility leukocyte antigen (HLA)-B27 subtypes. The only detected amino acid change between the main HLA-B27.1 subtype and B27d is that of Try/sub 59/ to His/sub 59/. Position 59 has not been previously found to vary among class I HLA or H-2 antigens. Such substitution accounts for the reported isoelectric focusing pattern of this variant. HLA-B27d is the only B27 variant found to differ from other subtypes by a single amino acid replacement. The nature of the change is compatible with its origin by a point mutation from HLB-B27.1. Because B27d was found only American blacks and in no other ethnic groups, it is suggested that this variant originated as a result of a mutation of the B27.1 gene that occurred within the black population. Structural analysis of B27d was done by comparative mapping. Radiochemical sequencing was carried out with /sup 14/C-labeled and /sup 3/H-labeled amino acids.

  3. The asymmetric protein expression hypothesis - Explaining the unilaterality of HLA-B27-positive acute anterior uveitides.

    Clarke, Margo S; Plouznikoff, Alexandre; Deschênes, Jean

    2016-03-01

    For reasons still unclear, most HLA-B27-positive acute anterior uveitides occur unilaterally. Building upon the growing literature showing that left-right asymmetry exist at the biomolecular and at the cellular levels, we propose a new hypothesis to explain why HLA-B27-positive acute anterior uveitides tend to affect one eye selectively. We postulate that left and right uveal tissue may present quantitatively and qualitatively different proteins to the immune system, capable to trigger an autoimmune response, and that other variables, including anatomical, cellular and molecular barriers, as well as our own eye-derived immunological tolerance and immune suppressive intraocular microenvironment may also be unequally distributed, and impact differently the immune privileges of the left and right eye. These same quantitative and qualitative differences might also explain why HLA-B27-positive acute anterior uveitides can flip-flop between the left and the right eye, after the first attack. By trying to figure out why one eye is targeted by an autoimmune reaction while the other is clinically unaffected, we might be able to better understand how and why an autoimmune reaction starts. Hopefully, this will help us devise better treatments for ocular autoimmune diseases, and contribute to the management of autoinflammatory conditions with a marked asymmetric clinical presentation in other fields. PMID:26880626

  4. Structural analysis of an HLA-B27 functional variant, B27d detected in American blacks

    The structure of a new functional variant B27d has been established by comparative peptide mapping and radiochemical sequencing. This analysis complete the structural characterization of the six know histocompatibility leukocyte antigen (HLA)-B27 subtypes. The only detected amino acid change between the main HLA-B27.1 subtype and B27d is that of Try59 to His59. Position 59 has not been previously found to vary among class I HLA or H-2 antigens. Such substitution accounts for the reported isoelectric focusing pattern of this variant. HLA-B27d is the only B27 variant found to differ from other subtypes by a single amino acid replacement. The nature of the change is compatible with its origin by a point mutation from HLB-B27.1. Because B27d was found only American blacks and in no other ethnic groups, it is suggested that this variant originated as a result of a mutation of the B27.1 gene that occurred within the black population. Structural analysis of B27d was done by comparative mapping. Radiochemical sequencing was carried out with 14C-labeled and 3H-labeled amino acids

  5. Application of a Simple In-House PCR-SSP Technique for HLA-B* 27 Typing in Spondyloarthritis Patients

    Devraj J. Parasannanavar

    2013-01-01

    Full Text Available Background. Microlymphocytotoxicity (MLCT and flowcytometry (FC are the conventional serological methods to detect HLA-B* 27. Due to some disadvantages in these methods, most of the HLA laboratories have now switched over to molecular methods. Molecular techniques based on commercial kits are expensive; as such many laboratories with limited funds in developing countries cannot afford these techniques. Aims. Our main aim was to standardize a simple inexpensive in-house PCR-SSP technique for HLA-B* 27 typing. Materials and Methods. Sequence Specific primers were designed to amplify all the subtypes of B* 27 using IMGT-HLA sequence database. Accuracy was checked by retyping of 90 PCR-SSOP typed controls. Results. The presence of 149 bp specific band with control band on 2% agarose gel showed B* 27 positivity. No discrepancies were found when compared with PCR-SSOP results. The frequency of HLA-B* 27 was found to be significantly increased (68.75% versus 4.40%, O.R 46.909: P value 6.62E-32 among 700 SpA patients as compared to controls. Clinically, 54% of patients had polyarticular arthritis with SI joints involvement (68% and restricted spine flexion (60%. Conclusion. In-house PCR-SSP technique is very simple and inexpensive technique to detect B* 27 allele, which was strongly associated with SpA patients from Western India.

  6. 广东潮州地区强直性脊柱炎患者 HLA-B27的检测与临床相关性研究%Investigation of the relationship between clinical manifestation of ankylosing spondylitis and HLA-B27 antigen in Chaozhou, Guangdong Province

    刘潮坚; 蔡拉加; 石昭宏; 林剑雄

    2013-01-01

      目的检测强直性脊柱炎(Ankylosing spondylitis,AS)患者 HLA-B27抗原表达情况,并分析其与临床情况的关系.方法选择我院2008年5月到2011年5月期间150例 AS 患者,按 HLA-B27抗原表达分为两组:HLA-B27阳性组(n=108)及 HLA-B27阴性组(n=42).分别检测两组血标本血沉、C 反应蛋白、α1-酸性糖蛋白水平,并检查心电图、骨盆正位片和腰椎正侧位,比较两组的发病年龄、性别比、临床症状.结果① HLA-B27阴性组以31~45岁年龄段为主(占阴性组52.4%),阳性组以16~30岁年龄段为主(占阳性组57.4%);② HLA-B27阳性组较阴性组全身症状更为严重(P0.05);③ HLA-B27阴性组较多表现为在窦缓和/或传导阻滞,而 HLA-B27阳性组则较多表现为左室高电压(P0.05).结论 AS 患者临床症状与 HLA-B27抗原有很高的相关性,HLA-B27抗原阴性患者与阳性患者相比病情较轻,预后较好.%Objective To investigate the relationships between clinical manifestation of ankylosing spondylitis (AS) and HLA-B27 antigen by analyzing the expression level of HLA-B27 antigen. Methods During May 2008 and May 2011, 150 AS patients attended our study. According to the expression level of HLA-B27 antigen, these patients were classified into two groups: HLA-B27 negative group and HLA-B27 positive group. The erythrocyte sedimentation rate (ESR), CRP, and α1-AGP, electrocardiogram, anterioposterior radiograph of pelvis, anterior and lateral lumbar vertebral X ray were also examined. The age of onset, sex proportion, and clinical manifestation were compared in the two groups. Results ① HLA-B27 negative expression mainly ranged from 31 to 45 years old (52.4%), but positive expression group mainly ranged from 16 to 30 years old (57.4%). ② The rate of systemic symptom such as fever, night sweat, and fatigue were higher in HLA-B27 positive group than that in negative group(P0.05). ③ The major clinical symptom in HLA-B27 negative patients were sinus

  7. The Combination of MR Imaging and HLA-B27 on the Diagnosis of Sacroiliac Joints Lesions in Ankylosing Spondylitis%MRI与HLA-B27诊断强直性脊柱炎骶髂关节病变的联合应用

    王东; 江华堂; 陈武标; 吴永峻

    2012-01-01

    目的 探讨MRI与HLA-B27联合对诊断强直性脊柱炎(AS)骶髂关节(SIJ)病变的临床应用价值.方法 回顾性分析48例经临床确诊的AS的MRI和HLA-B27检测资料,并比较MRI与MRI联合HLA-B27对AS SIJ病变诊断的阳性率.结果 48例AS病例中,MRI检查诊断AS的阳性率为79.2%;HLA-B27检测阳性率为89.6%;MRI联合HLA-B27检测阳性为91.7%; MRI联合HLA-B27诊断AS的阳性率高于单纯MRI检查组,其差异有统计学意义(x2=11.759,P<0.01).结论 MRI与HLA-B27联合可提高AS诊断的阳性率,有利于AS的早期发现、及时治疗.%Objective To investigate the clinical value of combination of MR imaging and HLA-B27 on the diagnosis of sacroiliac joints (SIJ) lesions in ankylosing spondylitis (AS). Methods The MRI and HLA-B27 data of 48 AS cases proved by clinical examination were retrospectively analyzed. The positive rates in detecting the SIJ lesions of AS were compared between MRI and combination MRI and HLA-B27. Results Among the 48 patient of AS, the positive rate of MRI diagnosis was 79.2%, the positive rate of HLA-B27 examination was 89.6%, the positive rate of MRI combined with HLA-B27 was 91.7%. The positive rate of MRI combined with HLA-B27 was higher than that of MRI, which were showing significant difference (X2=1.759, P < 0.01). Conclusion The combination of MRI and HLA-B27 examination can increase the positive rate of AS diagnosis, which may be helpful for AS early detection and prompt treatment.

  8. Antibody activity in ankylosing spondylitis sera to two sites on HLA B27.1 at the MHC groove region (within sequence 65-85), and to a Klebsiella pneumoniae nitrogenase reductase peptide (within sequence 181-199)

    1990-01-01

    74 overlapping peptides of varying lengths from Klebsiella pneumoniae nitrogenase reductase (residues 181-199) and from the HLA B27.1 molecule (residues 65-85) were synthesized and tested by ELISA against sera from HLA B27+ ankylosing spondylitis (AS) patients, and sera from HLA B27+ and HLA B27- healthy first-degree relatives. Antibody activity in AS sera to Klebsiella peptides of four to eight amino acids was maximal with the peptide NSRQTDR. Activity to HLA B27 peptides was maximal with th...

  9. The value of combination of magnetic resonance imaging and HLA-B27 in early diagnosis of ankylosing spondylitis%磁共振联合HLA-B27诊断早期强直性脊柱炎的应用价值

    孔庆聪; 邵硕; 刘卫敏; 王晓红; 林云崖; 单鸿

    2012-01-01

    目的 探讨MRI联合HLA-B27抗原检测骶髂关节强直性脊柱炎(AS)早期诊断的临床价值.方法 回顾性分析经临床确诊的35例早期AS患者,均行骶髂关节X线、MRI检查及HLA-B27抗原检测,并分别比较骶髂关节MRI、HLA-B27、骶髂关节MRI联合HLA-B27的阳性率.结果 35例早期强直性脊柱炎患者中,通过MRI检查诊断早期强直性脊柱炎的阳性率为74.3%(26/35例),HLA-B27阳性率为88.6%(31/35例),骶髂关节MRI联合HLA-B27的阳性率为97.1%(34/35例),其中MRI联合HLA-B27的阳性检出率高于MRI有统计学意义(P=0.013),MRI与HLA-B27、MRI联合HLA-B27HLA-B27的阳性检出率比较无统计学意义(P>0.05).结论 骶髂关节MRI联合HLA-B27检查能提高早期强直性脊柱炎的阳性检出率,有利于临床早期诊断、早期治疗.%Objective To explore the clinical value of combination of Magnetic Resonance Imaging! MRI) and HLA-B27 in early diagnosis of ankylosing spondylitis (AS). Methods 35 patients with early AS proven by clinical examination were included in this study. All patients underwent X-ray,MRI and HLA-B27 examination before treatment. The positive rates in detecting AS were compared between MRI and combination MR imaging and HLA-B27. Results Among the 35 patients of early AS, only 26 (74. 3%) were diagnosed to be early AS by MRI,the positive rate of HAL-B27 was 88. 6% ,the positive rate of MRI combined with HLA-B27 was 97. 1% . The positive rate of MRI combined with HLA-B27 was significantly higher than that of MRI (P = 0. 013 ) . While thepositive rates were not statistically significant between MRI and HLA-B27,and between MRI combined with HLA-B27and HLA-B27 alone (P>0. 05). Conclusion Combination of MRI examination and HLA-B27 can improve the detecting rate early of AS.

  10. Comparison of Clinical Features in Ankylosing Spondylitis Patients with HLA-B27 Negative Expression and Positive Expression%HLA-B27阴性和阳性强直性脊柱炎患者临床情况比较

    刘波; 胡友红

    2011-01-01

    Objective To compare the clinical situation of the patients diagnosed as ankylosing spondylitis( AS ) with negative expression and positive expression of human leukocyte antigen B27( HLA-B27 ).Methods Use flow cytometry to measure HLA-B27 among the 301 patients diagnosed as AS in our hospital . And a comparative analysis has been done between the HLA-B27 negative and HLA-B27 positive patients.Results ①The incidence in male was significantly higher than in female in HLA-B27 negative expression group and positive expression group. The average onset age of HLA-B27-negative patients are older than those of the positive ones of the same sex; ②The age of the patients with HLA-B27 negative expression was mainly 31 ~45,and the positive ones 16 ~30. ③The major first symptoms of both the positive and negative patients were axial and peripheral arthritis. The major clinical symptoms include low back pain and hip pain. The rate of systemic symptom such as fever, night sweat and fatigue in negative patients was significant lower than that in the positive group. Conclusion The average age and onset age of HLA-B27-negative patients are older than those of the positive ones of the same sex,and part of HLA-B27 negative patients'condition was relatively mild. The different clinical features of HLA-B27 negative expression and positive expression patients call for due attention in diagonosing suspected AS patients with negative HLA-B27 expression.%目的 比较HLA-B27检测阴性与阳性的确诊强直性脊柱炎(AS)患者临床情况.方法 用流式细胞仪对在本院确诊的301例AS患者进行人类白细胞抗原B27(HLA-B27)检测,并对HLA-B27阴性和阳性患者进行比较研究.结果 ①HLA-B27阴性和阳性患者男性发病率明显高于女性,阴性与同性别阳性患者平均发病年龄明显偏晚.②HLA-B27阴性患者以31~45岁为主,阳性患者以16~30岁为主.③HLA-B27阴性和阳性患者首发症状均以中轴关节炎和外周关节

  11. Value of Combination Detection of HLA-B27 and Sacroiliac Joint CT Scanning in Diagnosis of Ankylosing Spondylitis%骶髂关节CT及HLA-B27联合检测在强直性脊柱炎诊断中的价值

    王红; 金笛; 肖红霞

    2012-01-01

    Objective To study the relativity between the feature of sacroiliac joint CT and the positive rate of HLA - B27 in the e-raly diogonosis of ankylosing spondylitis, in order to improve the diagnostic level of the ankylosing spondylitis. Methods Sacroiliac joint CT features and the positive rate of HLA - B27 of 162 cases diagnosed as ankylosing spondylitis were collectively analysed. Results 6. 2% of sacroiliac joints CT of 162 patients were normal but 93. 8% abnormal. Positive rate of HLA - B27 was 84% . Cases simultaneously existing both HLA - B27 posibility and abnormity of sacroiliac joints CT were 79% , but cases of HLA - B27 negativity and abnormity of sacroiliac joints CT were 14. 8% . Cases of HLA - B27 positive but sacroiliac joints CT normal were 6. 2% . Compared with the result of HLA - B27 and sacroilial joint CT,x2 =2.118,P>0.01. Conclusion HLA - B27 and sacroiliac joints CT are important reference indicators for diagnosing ankylosing spondylitis. The correspondence between the results of HLA - B27 and the sacroiliac joint CT is high. There is no significant differences in two kinds of results. Detection of HLA - B27 in combination of clinical symptoms is beneficial for early diagnosis. Characteristics of sacroiliac joints CT could be used as important foundation in early, developing and late phases.%目的 探讨骶髂关节CT及HLA-B27联合检测在强直性脊柱炎早期诊断中的应用,以提高强直性脊柱炎的诊断水平.方法 分析经临床诊断为强直性脊柱炎的162例患者HLA-B27的阳性率及骶髂关节CT的特点,对其结果进行总结性分析.结果 162例患者中骶髂关节CT异常者占93.8%.HLA-B27阳性率为85.2%.HLA-B27阳性及骶髂关节CT异常表现并存占79%;HLA-B27阴性、骶髂关节异常占14.8%;HLA-B27阳性而骶髂关节正常者6.2%.HLA-B27与骶髂关节CT两种结果比较,x2 =2.118,P >0.01.结论 HLA-B27及骶髂关节CT是诊断强直性脊柱炎的重要参考指标.诊断AS的HLA-B

  12. HLA-B27检测应用于强直性脊柱炎的诊断价值分析

    梁小亮

    2011-01-01

    目的:探讨HLA-B27检测对强直性脊柱炎的诊断价值.方法:58例患者作为试验组,另选择58例健康人士作为对照组,对比分析两组HLA-B27阳性率试结果:HLA-B27阳性率实验组为91.37%,对照组为6.90%,两组比较差异有显著性(X2=79.472,P<0.01).结论:HLA-B27检测用于强直性脊柱炎的诊断,可提供重要的参考指标,从而进一步提高确诊率.

  13. Interaction between HLA-B60 and HLA-B27 as a Better Predictor of Ankylosing Spondylitis in a Taiwanese Population.

    James Cheng-Chung Wei

    Full Text Available Ankylosing spondylitis (AS is a form of chronic inflammatory spondyloarthritis (SpA that causes pain and stiffness in spines or joints. Human leukocyte antigen B27 (HLA-B27 and B60 (HLA-B60 have been reported as major genetic risk factors of AS. In addition, rs13202464, located on major histocompatibility complex (MHC region, showed high sensitivity (98.7% and specificity (98.0% for HLA-B27.The aim of our study is to test whether the interaction between HLA-B60 and HLA-B27 (rs13202464 can serve as a better predictor of AS. We have genotyped HLA-B60 and rs13202464 among 471 patients with AS and 557 healthy subjects. Combined risk factors were investigated to test the biological interaction.Our results indicated that the relative risk (RR for HLA-B27+/HLA-B60- was 152 (95% CI 91 to 255 and it increased to 201 (95% CI 85 to 475 in HLA-B27+/HLA-B60+ patients (with HLA-B27-/HLA-B60- as reference. Combinational analysis of two risk factors (HLA-B27+/HLA-B60+ showed a relative excess risk due to interaction (RERI of 46.79 (95% CI: -117.58 to 211.16, attributable proportion (AP of 0.23 (95% CI: -0.41 to 0.88 and a synergy index (S of 1.31 (95% CI: 0.56 to 3.04.In conclusion, genetic interaction analysis revealed that the interaction between HLA-B60 and HLA-B27 is a better marker for the risk of AS susceptibility in a Taiwanese population.

  14. 微量淋巴细胞毒法检测HLA-B27试验的质控体会

    岳文勇

    2008-01-01

    @@ 国内外研究表明,强直性脊柱炎(AS)患者HLA-B27抗原阳性率为83%~95.19%[1,2].对患者进行HLA-B27检测已经成为临床排除、提示或预防强直性脊柱炎的一个重要的辅助手段.

  15. HLA-B 27在强直性脊柱炎诊断中的应用效果评价

    张斓

    2015-01-01

    目的 探讨HLA-B27在强直性脊柱炎(AS)诊断中的应用效果.方法 随机抽取53例AS患者的临床资料,作为观察组.选取同时期进行健康体检的志愿者53例作为对照组.抽取被研究者空腹血2 mL,均匀混合,抗凝完全.存于4℃冰箱,4 d内检测均有效.采取微量细胞毒法检测HLA-B27水平.观察HLA-B27阳性检出率.结果 观察组患者HLA-B27阳性检出率为92.45%,对照组HLA-B27阳性检出率为0.00%,2组HLA-B27阳性检出率比较,差异有统计学意义(P<0.05).观察组患者HLA-B 27诊断敏感度为92.45%(49/53),特异度为100.00%(53/53),诊断正确率为96.23%.结论 HLA-B27可作为AS的诊断依据,辅助临床诊断.

  16. HLA-B27-Transfected (Salmonella Permissive) and HLA-A2-Transfected (Salmonella Nonpermissive) Human Monocytic U937 Cells Differ in Their Production of Cytokines

    Ekman, Päivi; Saarinen, Marja; He, Qiushui; Gripenberg-Lerche, Christel; Grönberg, Alvar; Arvilommi, Heikki; Granfors, Kaisa

    2002-01-01

    The cytokine secretion of the Salmonella-permissive, HLA-B27-positive U937 cells was examined, as it was previously shown that these cells kill Salmonella less efficiently than controls. Salmonella-permissive U937 cells showed upregulated production of interleukin 10 and to a lesser extent tumor necrosis factor alpha. HLA-B27-associated modulation of cytokine responses may have importance in the pathogenesis of reactive arthritis.

  17. HLA-B27 modulates intracellular growth of Salmonella pathogenicity island 2 mutants and production of cytokines in infected monocytic U937 cells.

    Shichao Ge

    Full Text Available BACKGROUND: Salmonella enterica serovar Enteritidis PT4 KS8822/88 replicates rapidly in HLA-B27-transfected human monocytic U937 cells. In this process, Salmonella pathogenicity island 2 (SPI-2 genes play a crucial role. Our previous study indicated that 118 Salmonella genes, including 8 SPI-2 genes were affected by HLA-B27 antigen during Salmonella infection of U937 cells. METHODS/PRINCIPAL FINDINGS: To further investigate Salmonella replication in HLA-B27-positive U937 monocytic cells, two SPI-2 genes, ssaS and sscA up-regulated most during Salmonella infection of HLA-B27-transfected U937 cells, were mutated by using one-step gene disruption method. Intracellular survival and replication of the mutants in the U937 cells was compared to that of the wild type strain. Surprisingly, the two mutated strains replicated significantly more than the wild type bacteria in HLA-B27-transfected cells. Secretion of tumor necrosis factor alpha (TNF-α and interleukin 10 (IL-10 was significantly induced during the infection of HLA-B27-transfected U937 cells with the mutants. The results indicated that the certain SPI-2 genes in wild type bacteria suppress Salmonella intracellular growth and production of cytokines in infected HLA-B27-transfected cells. HLA-B27-associated modulation of Salmonella SPI-2 genes and cytokine production may have importance in the persistent infection of the bacteria and the pathogenesis of reactive arthritis. CONCLUSIONS: The study provides evidence that certain virulence factors of pathogens can reduce the intracellular growth in the host cells. We suggest that the limiting intracellular growth might be a strategy for persistence of bacteria in host cells, keeping a balance between pathogenic growth and pathogenesis.

  18. Peptide Handling by HLA-B27 Subtypes Influences Their Biological Behavior, Association with Ankylosing Spondylitis and Susceptibility to Endoplasmic Reticulum Aminopeptidase 1 (ERAP1)*

    García-Medel, Noel; Sanz-Bravo, Alejandro; Alvarez-Navarro, Carlos; Gómez-Molina, Patricia; Barnea, Eilon; Marcilla, Miguel; Admon, Arie; de Castro, José A. López

    2014-01-01

    HLA-B27 is strongly associated with ankylosing spondylitis (AS). We analyzed the relationship between structure, peptide specificity, folding, and stability of the seven major HLA-B27 subtypes to determine the role of their constitutive peptidomes in the pathogenicity of this molecule. Identification of large numbers of ligands allowed us to define the differences among subtype-bound peptidomes and to elucidate the peptide features associated with AS and molecular stability. The peptides iden...

  19. 闽南地区强直性脊柱炎与HLA-B27关联性的观察

    洪素云; 周娟娟; 裴斌; 谢金镇

    2005-01-01

    目的:探讨闽南地区HLA-B27对强直性脊柱炎的诊断意义.方法:用特异性HLA-B27抗血清对临床疑似强直性脊柱炎的患者 1 500 例和 1 500 名健康者进行HLA-B27检测.结果:疑似强直性脊柱炎患者 1 500 例,其中男性 1 212 例,女性288 例,男女比例约为4:1.B27阳性率为 67.07%,其中男性 70.05%(849/1212),女性 54.51%.发病年龄主要集中在青少年时期.1 500 名健康者中HLA-B27阳性39例,阳性率为 2.6%.结论:AS与HLA-B27相关性强,HLA-B27血清学检测对强直性脊柱炎的诊断和鉴别诊断具有重要意义.

  20. Human Leukocyte Antigen (HLA) B27 Allotype-Specific Binding and Candidate Arthritogenic Peptides Revealed through Heuristic Clustering of Data-independent Acquisition Mass Spectrometry (DIA-MS) Data.

    Schittenhelm, Ralf B; Sivaneswaran, Saranjah; Lim Kam Sian, Terry C C; Croft, Nathan P; Purcell, Anthony W

    2016-06-01

    Expression of HLA-B27 is strongly associated with ankylosing spondylitis (AS) and other spondyloarthropathies. While this is true for the majority of HLA-B27 allotypes, HLA-B*27:06 and HLA-B*27:09 are not associated with AS. These two subtypes contain polymorphisms that are ideally positioned to influence the bound peptide repertoire. The existence of disease-inducing peptides (so-called arthritogenic peptides) has therefore been proposed that are exclusively presented by disease-associated HLA-B27 allotypes. However, we have recently demonstrated that this segregation of allotype-bound peptides is not the case and that many peptides that display sequence features predicted to favor binding to disease-associated subtypes are also capable of being presented naturally by protective alleles. To further probe more subtle quantitative changes in peptide presentation, we have used a combination of data-independent acquisition (DIA) and multiple reaction monitoring (MRM) mass spectrometry to quantify the abundance of 1646 HLA-B27 restricted peptides across the eight most frequent HLA-B27 allotypes (HLA-B*27:02-HLA-B*27:09). We utilized K means cluster analysis to group peptides with similar allelic binding preferences across the eight HLA-B27 allotypes, which enabled us to identify the most-stringent binding characteristics for each HLA-B27 allotype and further refined their existing consensus-binding motifs. Moreover, a thorough analysis of this quantitative dataset led to the identification of 26 peptides, which are presented in lower abundance by HLA-B*27:06 and HLA-B*27:09 compared with disease-associated HLA-B27 subtypes. Although these differences were observed to be very subtle, these 26 peptides might encompass the sought-after arthritogenic peptide(s). PMID:26929215

  1. Microarray Analysis of Response of Salmonella during Infection of HLA-B27- Transfected Human Macrophage-Like U937 Cells

    Hinton Jay CD

    2010-07-01

    Full Text Available Abstract Background Human leukocyte antigen (HLA-B27 is strongly associated with the development of reactive arthritis (ReA in humans after salmonellosis. Human monocytic U937 cells transfected with HLA-B27 are less able to eliminate intracellular Salmonella enterica serovar Enteritidis than those transfected with control HLA antigens (e.g. HLA-A2. To investigate further the mechanisms by which HLA-B27-transfected cells allow increased replication of these bacteria, a DNA-based microarray was used for comparative genomic analysis of S. Enteritidis grown in HLA-B27- or HLA-A2-transfected cells. The microarray consisted of 5080 oligonucleotides from different serovars of Salmonella including S. Enteritidis PT4-specific genes. Bacterial RNA was isolated from the infected HLA-B27- or HLA-A2-transfected cells, reverse-transcribed to cDNA, and hybridized with the oligonucleotides on the microarrays. Some microarray results were confirmed by RT-PCR. Results When gene expression was compared between Salmonella grown in HLA-B27 cells and in HLA-A2 cells, 118 of the 4610 S. Enteritidis-related genes differed in expression at 8 h after infection, but no significant difference was detectable at 2 h after infection. These differentially expressed genes are mainly involved in Salmonella virulence, DNA replication, energy conversion and metabolism, and uptake and metabolism of nutrient substances, etc. The difference suggests HLA-B27-dependent modulation of Salmonella gene expression, resulting in increased Salmonella replication in HLA-B27-positive cells. Among the up-regulated genes were those located in Salmonella pathogenicity island (SPI-2, which play a central role in intracellular survival and replication of Salmonella. Conclusions This is the first report to show the regulation of Salmonella gene expression by HLA-B27 during infection of host cells. This regulation probably leads to increased Salmonella survival and replication in HLA-B27-positive

  2. Effect of HLA-B*27 and its subtypes on clinical manifestations and severity of ankylosing spondylitis in Iranian patients.

    Sasan Fallahi

    2013-12-01

    Full Text Available The aim of this study was to assess the role of HLA-B*27 and it's subtypes in determining severity and clinical manifestations of ankylosing spondylitis (AS.A total of 163 AS patients were assessed for clinical manifestations and severity using structured questionnaires. HLA-B*27 screening and B*27 sub-typing were performed by PCR.One hundred twenty two patients (74.8% were B*27 positive. The male to female ratio, peripheral arthritis, steroid use, intense dorsal kyphosis and decrease of cervical slope had a significantly higher frequency in B*27 positive patients compared to B*27 negative ones (p=0.01, 0.001, 0.01, 0.04 and 0.04, respectively. However, the age of diagnosis was significantly lower in B*27 positive patients (p=0.005. Trend in uveitis and some severity markers including: BASMI and ASQoL were toward higher values in B*27 positive group with no significant difference. After controlling confounding variables, significant relationship was found only between B*27 and BASMI (p=0.01. B*27 subtypes in patients were included B*2705: 48.4%, B*2702: 42.6%, B*2704: 5.7% and B*2707: 3.3%. No significant differences were seen for severity markers and clinical manifestations between subtypes; although trend toward lower values of severity markers, less intense dorsal kyphosis and less decrease of cervical slope were observed in B*2704 and B*2707 versus other polymorphisms.Clinical features and severity of AS is influenced by HLA-B*27. Trend toward higher severity markers in B*2705 and B*2702 versus other polymorphisms might be subject of interest for evaluation in other ethnicities with concentration to other novel susceptibility genes co-inherited in each B*27 subtype.

  3. Detection of HLA-B27 by flow cytometry and it's clinical evaluation: Analysis of 112 cases%流式细胞术检测HLA-B27及其临床意义(附112例分析)

    张臣青; 陈志哲

    2005-01-01

    目的通过流式细胞术检测外周血白细胞HLA-B27的表达,并探讨其对强直性脊柱炎(AS)的诊断价值.方法利用流式细胞仪(FCM)检测33例AS患者、34例背痛及腰腿痛患者及45例健康体检者的外周血HLA-B27表达.结果33例AS患者中,HLA-B27阳性30例(90.9%);34例背痛腰腿痛患者中,HLA-B27阳性4例(11.76%);45例健康人群中,HLA-B27阳性4例(8.9%).结论AS患者HLA-B27阳性率明显增高,HLA-B27检测可视为临床支持AS诊断的重要依据.

  4. Analysis of HLA-B15 and HLA-B27 in spondyloarthritis with peripheral and axial clinical patterns

    Londono, John; Santos, Ana Maria; Peña, Paola; Calvo, Enrique; Espinosa, Luis R; John D Reveille; Vargas-Alarcon, Gilberto; Jaramillo, Carlos A.; Valle-Oñate, Rafael; Avila, Mabel; Romero, Consuelo; Medina, Juan F.

    2015-01-01

    Objective Human leucocyte antigen (HLA) B27 and HLA-B15 are associated with spondyloarthritis (SpA). Recent Assessment of SpondyloArthritis international Society (ASAS) criteria emphasise a distinction between SpA with axial and peripheral patterns. We analysed whether HLA-A, HLA-B and HLA-DRB1 alleles could associate with these patterns. Methods We studied 100 healthy individuals and 178 patients with SpA according to European Spondyloarthropathy Study Group (ESSG) criteria. Patients were th...

  5. HLA B27检测在强直性脊柱炎临床诊断中的意义

    黄翠珍; 蔡桂丰

    2005-01-01

    目的:评价 HLA B27检测在强直性脊柱炎 (AS)临床诊断中的作用.方法:应用磁珠酶联免疫检验试剂对怀疑患有 AS及其相关疾病的 265人作 HLA B27检测.采用 1984年的纽约修订的 AS诊断标准作为本研究的 AS诊断标准.结果:受检 265例中, HLA B27阳性检出率为 27.9%(74/265).高阳性率集中在轻壮年,并且男性阳性率 >女性阳性率(P< 0.01).按照纽约标准诊断为 AS的 69例患者中, HLA B27阳性率为 88.4%(61/69). HLA B27检测对诊断 AS的敏感度为 88.4%,特异度为 93.4%(183/196),诊断正确率为 92.1% (244/265).结论:对疑似 AS患者进行 HLA B27检测是必要的. HLA B27磁珠酶联免疫检验法对 AS诊断有高敏感性和高特异性,不失为当前 AS诊断的较好的实验指标.

  6. Endoplasmic reticulum aminopeptidase-1 alleles associated with increased risk of ankylosing spondylitis reduce HLA-B27 mediated presentation of multiple antigens.

    Seregin, Sergey S; Rastall, David P W; Evnouchidou, Irini; Aylsworth, Charles F; Quiroga, Dionisia; Kamal, Ram P; Godbehere-Roosa, Sarah; Blum, Christopher F; York, Ian A; Stratikos, Efstratios; Amalfitano, Andrea

    2013-12-01

    Ankylosing spondylitis (AS) is a chronic systemic arthritic disease that leads to significant disability and loss of quality of life in the ∼0.5% of the worldwide human population it affects. There is currently no cure for AS and mechanisms underlying its pathogenesis remain unclear. AS is highly genetic, with over 70% of the genetic risk being associated with the presence of HLA-B27 and endoplasmic reticulum aminopeptidase-1 (ERAP1) alleles. Furthermore, gene-gene interactions between HLA-B27 and ERAP1 AS risk alleles have recently been confirmed. Here, we demonstrate that various ERAP1 alleles can differentially mediate surface expression of antigens presented by HLA-B27 on human cells. Specifically, for all peptides tested, we found that an ERAP1 variant containing high AS risk SNPs reduced the amount of the peptide presented by HLA-B27, relative to low AS risk ERAP1 variants. These results were further validated using peptide catalysis assays in vitro, suggesting that high AS risk alleles have an enhanced catalytic activity that more rapidly destroys many HLA-B27-destined peptides, a result that correlated with decreased HLA-B27 presentation of the same peptides. These findings suggest that one mechanism underlying AS pathogenesis may involve an altered ability for AS patients harboring both HLA-B27 and high AS risk ERAP1 alleles to correctly display a variety of peptides to the adaptive arm of the immune system, potentially exposing such individuals to higher AS risk due to abnormal display of pathogen or self-derived peptides by the adaptive immune system. PMID:24028501

  7. Concerted in vitro trimming of viral HLA-B27-restricted ligands by human ERAP1 and ERAP2 aminopeptidases.

    Elena Lorente

    Full Text Available In the classical human leukocyte antigen (HLA class I antigen processing and presentation pathway, the antigenic peptides are generated from viral proteins by multiple proteolytic cleavages of the proteasome (and in some cases other cytosolic proteases and transported to the endoplasmic reticulum (ER lumen where they are exposed to aminopeptidase activity. In human cells, two different ER-resident enzymes, ERAP1 and ERAP2, can trim the N-terminally extended residues of peptide precursors. In this study, the possible cooperative effect of generating five naturally processed HLA-B27 ligands by both proteases was analyzed. We identified differences in the products obtained with increased detection of natural HLA-B27 ligands by comparing double versus single enzyme digestions by mass spectrometry analysis. These in vitro data suggest that each enzyme can use the degradation products of the other as a substrate for new N-terminal trimming, indicating concerted aminoproteolytic activity of ERAP 1 and ERAP2.

  8. Multiple sclerosis and HLA-B27 negative sacroiliitis in a Crohn’s disease patient

    K.H. Katsanos, N. Tzambouras, E.V. Tsianos

    2007-03-01

    Full Text Available SUMMARY A relationship between inflammatory bowel disease and MS is supported by a higher than expected coexistence of these diseases among families and individuals. A 32 year-old male with Crohn’s disease of the terminal ileum diagnosed 4 years earlier and HLA-B27 bilateral sacroiliitis diagnosed two years earlier, was admitted to our hospital because of an acute episode of blurred vision. In addition the patient complained of urine incontinence. Before this admission the patient had been elsewhere administered three doses of Remicade and 16mg of methylprednisolone p.os. During admission the diagnosis of multiple sclerosis was made (MRI and IgG Index and Remicade was discontinued. The patient was started on therapy with interferon-beta for MS, oxybutynin hydrochloride (10mg/day for urine incontinence, prednizolone (10mg/day, methotrexate (10mg/week and azathioprine (100mg/day for Crohn’s disease and is now in excellent clinical status. To the best of our knowledge this is one of the very rare cases of Crohn’s disease with HLA-B27 negative sacroiliitis preceding multiple sclerosis diagnosis. Key words: Crohn’s disease, inflammatory bowel disease, ulcerative colitis, multiple sclerosis, Remicade

  9. Combined effects of ankylosing spondylitis-associated ERAP1 polymorphisms outside the catalytic and peptide-binding sites on the processing of natural HLA-B27 ligands.

    Martín-Esteban, Adrian; Gómez-Molina, Patricia; Sanz-Bravo, Alejandro; López de Castro, José A

    2014-02-14

    ERAP1 polymorphism involving residues 528 and 575/725 is associated with ankylosing spondylitis among HLA-B27-positive individuals. We used four recombinant variants to address the combined effects of the K528R and D575N polymorphism on the processing of HLA-B27 ligands. The hydrolysis of a fluorogenic substrate, Arg-528/Asp-575 ERAP1 was a major determinant of the abundance of these peptides in vivo. The hydrolysis of fluorogenic and peptide substrates by an HLA-B27 ligand or a shorter peptide, respectively, was increasingly inhibited as a function of ERAP1 activity, indicating that residues 528 and 575 affect substrate inhibition of ERAP1 trimming. The significant and complex effects of co-occurring ERAP1 polymorphisms on multiple HLA-B27 ligands, and their potential to alter the immunological and pathogenetic features of HLA-B27 as a function of the ERAP1 context, explain the epistatic association of both molecules in ankylosing spondylitis. PMID:24352655

  10. The Method for Detecting HLA-B27 by Flow Cytometer and Its Application Study about Acute Anterior Uveitis%流式细胞术检测HLA-B27及其在急性前葡萄膜炎诊治中的应用研究

    张新颖; 宋玉国; 石萍; 刘祥龙

    2009-01-01

    目的:建立流式细胞仪检测白细胞膜HLA-B27检测方法.研究急性前葡萄膜炎(AAU)患者外周血白细胞HLA-B27表达情况,比较AAU患者和正常人HLA-B27阳性率的差异性;研究HLA-B27阳性AAU病例合并强直性脊柱炎(AS)的发生率,和AAU复发情况,为临床诊断和治疗提供科学依据.方法:用异硫氰酸荧光素(FITC)标记HLA-B27单克隆抗体,对细胞膜表面HLA-B27抗原进行标记,然后用流式细胞仪检测HLA-B27阳性细胞.检测方法建立后,进行临床应用研究.按临床诊断标准,确定AAU研究病例,并进行合并AS的临床诊断,记录治疗后AAU复发情况.对各组病例分别检测HLA-B27,研究各组的差异性.结果:共检测分析了47例AAU病例,其中HLA-B27阳性病例30例,阳性率为63.83%.明显高于正常对照组(P<0.05).47例AAU病人中,合并AS的有18例,这18例中HLA-B27阳性率高达72.22%,明显高于单纯AAU病例的HLA-B27阳性率(41.38%)(P<0.05).30例HLA-B27阳性AAU病例中有25例被观察到复发情况,复发率为83.33%,明显高于HLA-B27阴性AAu病例的复发率(47.06%,8/17)(P<0.05).结论:检测HLA-B27有助于AAU的诊断,是一项有效的辅助诊断指标.对于HLA-B27阳性的AAU病例,要注意AS的合并发生,提高相关疾病的诊断水平.对于HLA-B27阳性病例,注意疾病的复发情况,发现复发及时就医治疗,以减少并发症的发生减缓病情进展.

  11. Analysis of the diagnostic significance of HLA-B27 in spondyloarthropathy and non-spondyloarthropathy: report of 4 817 cases%4817例HLA-B27检测对SpA和非SpA诊断价值的分析

    魏秋静; 李秋霞; 李齐光

    2011-01-01

    Objective: To study the application of HLA-B27 antigen detection in diagnosis of spondyloarthropathy (SpAs) in patients with low back pain.Methods: HLA-B27 antigen detection with complement-dependent lymphocytotoxicity was performed in 4414 patients who sought medical consultation with low back pain and 403 healthy controls.Analysis of HLA-B27 positive distributions in different gender and diseases ( SpA and have non-SpA) was performed.Results: The positive rate of HLA-B27 antigen was 49.81% in 4 414 patients with low back pain, while no significant difference revealed between genders (P > 0.05 ).Positive rates of HLA-B27 in patients with ankylosing spondylitis ( AS) , undifferentiated spondyloarthropathy (USpA) , Juvenile spondyloarthropathy (JSpAs), reactive arthritis (ReA), psoriatic arthritis (PsA) and non-SpAs were 90.07%, 28.85%, 68.81%, 52.94%, 67.74% and 19.33%, respectively, with significant differences with the healthy control group (P < 0.05).Conclusions: Positive HLA-B27 detection can be found in patients with non-SpAs, suggesting that HLA-B27 is not an independent diagnostic factor of SpA.%目的:探讨HLA-B27对腰背痛疑诊或待排除SpA患者的鉴别诊断价值.方法:采用补体依赖淋巴细胞毒试验(CDC)检测4 414例以腰背痛就诊的患者及403名健康对照者的HLA-B27,结合患者临床资料分析HLA-B27在不同性别和疾病(SpA和非SpA两组)中的阳性分布情况.结果:4 414例腰背痛疑诊或待排除SpA人群HLA-B27的阳性率为49.81%,男女HLA-B27阳性率比较差异无统计学意义(P>0.05);在SpA组AS、未分化型SpA(USpA)、幼年SpA(JSpA)、反应性关节炎(ReA)和银屑病关节炎(PsA) HLA-B27阳性率分别为90.07%、28.85%、68.81%、52.94%和67.74%.非SpA组HLA-B27阳性率为19.33%,与健康对照组比较差异有统计学意义(P<0.05).结论:非SpA患者HLA-B27也可能阳性,HLA-B27检测阳性不能单纯考虑SpA.

  12. 379例疑似强直性脊柱炎患者HLA-B27抗原检查结果分析

    孙维萍

    2007-01-01

    目的:本文是为了总结本地区强直性脊柱炎与HLA-B27抗原的相关情况.方法:我们采用的是淋巴细胞毒实验Teraski血清盘法.结果:379例疑似强直性脊柱炎患者的HLA-B27抗原检查阳性的有124例,阴性为255例.结论:本地区强直性脊柱炎与HLA-B27抗原有极强相关.

  13. The Application of HLA-B27 Detection in the Clinical Diagnosis of Ankylosing Spondylitis%HLA-B27检测在强直性脊柱炎临床诊断中的应用(附120例报告)

    章祖成; 施水潮; 钱海平; 严力生

    2002-01-01

    目的:评价HLA-B27检测在强直性脊柱炎(AS)临床诊断中的作用.方法:对怀疑患有AS及其相关疾病的120人作HLA-B27检测,采用纽约标准改良的AS诊断标准作为本研究的AS诊断标准.结果:本组受检120例中,HLA-B27(+)56例,占受检总数的46.67%,按本研究改良标准诊断为AS 53例, 其中HLA-B27(+)48例.本组16周岁以下13例受检者,HLA-B27(+)7例均符合JAS诊断标准.结论:HLA-B27检测对提高AS的诊断率和对轻型、早期AS病例的发现具有临床诊断价值.

  14. The clinical value of HLA-B27/B7 in the diagnosis of ankylosing spondysis%HLA-B27/B7抗原检测在诊断强直性脊柱炎中的临床价值

    罗玲; 严海燕; 罗晓红

    2013-01-01

    目的 探讨HLA-B27/B7抗原检测在诊断强直性脊柱炎(AS)中的临床价值.方法 选取144例门诊及住院高度疑似AS的患者,应用流式细胞仪对其标本进行HLA-B27/B7抗原检测.结果 HLA-B27抗原阳性率在青少年人群比较高,且男性显著高于女性,受检的144例患者,HLA-B27抗原阳性率为63.9%,低于确诊为AS的阳性率(91.1%).HLA-B27抗原检测对诊断AS的灵敏度为91.1%(82/90),特异度为81.5%(44/54),正确率为87.5%(126/144).结论 HLA-B27抗原与AS密切相关,对临床诊断AS很有帮助,用流式细胞术进行检测是较好的方法.

  15. Peptide handling by HLA-B27 subtypes influences their biological behavior, association with ankylosing spondylitis and susceptibility to endoplasmic reticulum aminopeptidase 1 (ERAP1).

    García-Medel, Noel; Sanz-Bravo, Alejandro; Alvarez-Navarro, Carlos; Gómez-Molina, Patricia; Barnea, Eilon; Marcilla, Miguel; Admon, Arie; de Castro, José A López

    2014-12-01

    HLA-B27 is strongly associated with ankylosing spondylitis (AS). We analyzed the relationship between structure, peptide specificity, folding, and stability of the seven major HLA-B27 subtypes to determine the role of their constitutive peptidomes in the pathogenicity of this molecule. Identification of large numbers of ligands allowed us to define the differences among subtype-bound peptidomes and to elucidate the peptide features associated with AS and molecular stability. The peptides identified only in AS-associated or high thermostability subtypes with identical A and B pockets were longer and had bulkier and more diverse C-terminal residues than those found only among non-AS-associated/lower-thermostability subtypes. Peptides sequenced from all AS-associated subtypes and not from non-AS-associated ones, thus strictly correlating with disease, were very rare. Residue 116 was critical in determining peptide binding, thermodynamic properties, and folding, thus emerging as a key feature that unified HLA-B27 biology. HLA-B27 ligands were better suited to TAP transport than their N-terminal precursors, and AS-associated subtype ligands were better than those from non-AS-associated subtypes, suggesting a particular capacity of AS-associated subtypes to bind epitopes directly produced in the cytosol. Peptides identified only from AS-associated/high-thermostability subtypes showed a higher frequency of ERAP1-resistant N-terminal residues than ligands found only in non-AS-associated/low-thermostability subtypes, reflecting a more pronounced effect of ERAP1 on the former group. Our results reveal the basis for the relationship between peptide specificity and other features of HLA-B27, provide a unified view of HLA-B27 biology and pathogenicity, and suggest a larger influence of ERAP1 polymorphism on AS-associated than non-AS-associated subtypes. PMID:25187574

  16. Increase of HLA-DRB1*0408 and -DQB1*0301 in HLA-B27 positive reactive arthritis

    Tuokko, J; Reijonen, H.; Ilonen, J; K. Anttila; Nikkari, S; Mottonen, T; Yli-Kerttula, U; Toivanen, A

    1997-01-01

    OBJECTIVE—To study HLA class II association in reactive arthritis.
METHODS—63 patients with reactive arth-ritis and 46 with rheumatoid arthritis were included in the study. HLA-DR alleles were determined by using a sequence specific PCR method. Oligonucleotide hybridisation was used for definition of DRB1*04 subtypes and DQB1 alleles. HLA-B27 was determined by standard microcytotoxity test or by PCR. HLA-B27 subtyping was made by sequencing.
RESULTS—46 (73%) of 63 patients with reactive arthr...

  17. 强直性脊柱炎患者血液HLA-B27的临床参考值的预测%The Predicted Clinical Reference Value of HLA-B27 in the Peripheral Venous Blood of Ankylosing Spondylitis Patients

    张金飞

    2012-01-01

    目的 以实时荧光定量PCR(FQ-PCR)检测可疑患者HLA-B27的定量水平,研究强直性脊柱炎(AS)与HLA-B27的相关性,明确HLA-B27的检测值范围并用于AS的确诊诊断.方法 针对本院2005年~2010年收集的168例骶髂关节及下腰部疼痛等症状疑似AS病例进行回顾性分析,获得所有病例外周静脉血标本,然后通过实时荧光定量PCR进行HLA-B27的定量检测,疑似病例分为AS患者、HLA-B27阳性的非AS患者、HLA-B27阴性的非AS患者三组,根据临床体征、影像学进行确诊病情,同时选取健康体检者52例外周静脉血标本的HLA B-27测定结果进行对照,统计分析各变量与AS存在之间的关系.结果 AS患者、HLA-B27阳性的非AS患者、HLA-B27阴性的非AS患者、健康体检者的HLA-B27循环阈值(ct)平均值分别为:26.3 copies/ml、17.5 copies/ml、6.2 copies/ml、4.9 copies/ml,单变量分析表明,HLA-B27与化脓性关节炎明显相关(P<0.05).结论 AS患者HLA-B27定量分析循环阈值(ct)的95%参考值范围为(17.3~35.3)copies/ml,HLA-B27定量分析可作为疾病发病的参照影响因素及预测因素.%A fhuorogenic quantitative polymerase chain reaction( FQ-PCR) was used to detect quantitative level of the suspicious ankylosing spondylitis patients, to detennine the relativity between ankylosing spondylitis and HLA-B27, and the predicted reference value got from this experiment was used to diagnose ankylosing spondylitia. Methods 168 suspicous ankylosing spondylitia cases with the pain of sacroiliac joints and lower waist collected from 2002 to 2010, was to do retrospective analysis, all the blood specimen was got to analyze the quantitative level of HLA-B27. All patients on the basis of clinic and this experiment were divided into 3 groups; AS patients, non-AS patients with HLA-B27-positive patients group, non-AS patients with HLA-B27-negative patients group. 52 cases were collected as control group. Statistical analysis was to analyze the

  18. 内蒙古地区疑似强直性脊柱炎患者HLA-B27抗原阳性率观察%Investigation of positive rate of HLA-B27 antigen in patients with suspected ankylosing spondylitis in Inner Mongolia

    托娅; 张军力

    2013-01-01

    Objective To investigate the relationship between suspected ankylosing spondylitis and HLA-B27 antigen in Inner Mongolia by detecting the positive rate and expression intensity of HLA-B27 antigen in 998 suspected AS patients, and evaluate its clinical significance. Methods The positive rate and the mean fluorescence intensity (MFI) of HLA-B27 antigen on the peripheral blood T lymphocytic detected by flow cytometer. Results Among the 998 suspected AS patients, the majority patients (77.35%) were aged from 21 to 50 years old, and the ratio between male and female was 1.4∶1. Among the all patients, the positive rate of antigen HLA-B27 was 28.86%, and the male and female patients' positive rates of HLA-B27 antigen were 33.05% and 23.02%, respectively (P<0.05). The MFI of male and female patients were significantly different (P<0.05). Conclusion The patients with AS in Inner Mongolia are strongly associated with HLA-B27 antigen. Detection of HLA-B27 antigen expression intensity in suspected AS patients with FCM is helpful to diagnosis and differential diagnosis of AS.%目的通过检测998例疑似强直性脊柱炎(Ankylosing spondylitis,AS)患者HLA-B27抗原阳性率和表达强度,了解内蒙古地区AS与HLA-B27抗原的相关性,并初步探讨其临床意义。方法采用流式细胞术检测患者外周血T淋巴细胞膜上的HLA-B27抗原和其平均荧光强度(mean fluorescence intensity,MFI)。结果998例疑似AS患者以21~50岁青壮年为主,占77.35%;男女比例为1.4∶1;998例疑似AS患者的HLA-B27抗原阳性率28.86%;男女患者HLA-B27抗原阳性率分别为33.05%和23.02%(P<0.05);男女阳性患者的MFI有明显差异(P<0.05)。结论内蒙古地区AS患者与HLA-B27抗原有着很强的相关性。采用流式细胞技术检测临床疑似AS患者T淋巴细胞HLA-B27表达强度有助于诊断和鉴别诊断AS。

  19. Complete sequence of HLA-B27 cDNA identified through the characterization of structural markers unique to the HLA-A, -B, and -C allelic series

    Szoets, H.; Reithmueller, G.; Weiss, E.; Meo, T.

    1986-03-01

    Antigen HLA-B27 is a high-risk genetic factor with respect to a group of rheumatoid disorders, especially ankylosing spondylitis. A cDNA library was constructed from an autozygous B-cell line expressing HLA-B27, HLA-Cw1, and the previously cloned HLA-A2 antigen. Clones detected with an HLA probe were isolated and sorted into homology groups by differential hybridization and restriction maps. Nucleotide sequencing allowed the unambiguous assignment of cDNAs to HLA-A, -B, and -C loci. The HLA-B27 mRNA has the structure features and the codon variability typical of an HLA class I transcript but it specifies two uncommon amino acid replacements: a cysteine in position 67 and a serine in position 131. The latter substitution may have functional consequences, because it occurs in a conserved region and at a position invariably occupied by a species-specific arginine in humans and lysine in mice. The availability of the complete sequence of HLA-B27 and of the partial sequence of HLA-Cw1 allows the recognition of locus-specific sequence markers, particularly, but not exclusively, in the transmembrane and cytoplasmic domains.

  20. Application of PCR-SBT method in HLA-B27 detection in AS patients%聚合酶链反应-直接测序法在强直性脊柱炎患者 HLA-B27检测中的应用

    王平均; 孙灵迪; 梅传忠; 武晓茜; 邵先安

    2015-01-01

    目的:探讨聚合酶链反应-直接测序法(polymerase chain reaction sequence-based typing,PCR-SBT)检测人类白细胞抗原 B27(human leukocyte antigen B27,HLA-B27)在强直性脊柱炎(ankylosing spondylitis,AS)临床诊断中的价值。方法应用 PCR-SBT 法和临床常用的 IMS-ELISA 法对120例疑似 AS 患者外周血进行 HLA-B27检测。以 SPSS17.0软件的配对χ2检验和受试者工作特征曲线下面积对两种方法检测 HLA-B27在 AS 诊断中的价值进行评价。结果120例疑似 AS 患者中,PCR-SBT 和 IMS-ELISA 法 HLA-B27检测阳性率分别为45.83%(55/120),37.50%(45/120)。两种方法检测 HLA-B27有统计学差异(P=0.031)。PCR-SBT 法敏感度和特异度分别为96.36%,96.92%;IMS-ELISA 法的敏感度和特异度分别为69.09%,89.23%。两者的 AUC 分别为0.966和0.792。结论与传统的 IMS-ELISA 法相比,在 AS 的临床诊断中 PCR-SBT 法检测 HLA-B27的敏感度和特异度更高,方法更优。%Objective The aim of this study was to compare PCR-SBT to IMS-ELISA in the HLA-B27 detection in the ankylosing spondylitis (AS)patients.Methods Simultaneously,PCR-SBT and IMS-ELISA were used to detect the HLA-B27 expression in peripheral blood samples which were suspected patients with AS from 120 cases.Chi square test of paired design and the area under curve of receiver operating characteristics of SPSS17.0 software were used to e-valuate the value of PCR-SBT and IMS-ELISA in HLA-B27 detection of AS patients.Results Among 120 cases of sus-pected patients with AS,the positive rates of HLA-B27 detected by PCR-SBT and IMS-ELISA were 45.83% (55/120),37.50%(45/120),respectively.There was statistical difference between the two methods in the HLA-B27 detec-tion (P =0.032).The sensibility and specificity of PCR-SBT were 96.36%,96.92%,respectively;while the sensibility and the specificity of IMS-ELISA were 69.09%,89.23%,respectively

  1. The HLA-B27,B7,B13 and B40 Antigen Expression of the Ankylosing Spondylitis and the Analysis of the Immunology Index%强直性脊柱炎患者HLA-B27、B7、B13、B40抗原表达及免疫学指标的分析

    李士军; 袁宏; 王滨; 曹华军

    2005-01-01

    目的探讨强直性脊柱炎患者HLA-B27、B7、B13、B40的抗原表达,发现抗原相互杂合与交叉反应,以及强直性脊柱炎患者的免疫学指标的变化.方法采用NIH微量淋巴细胞毒法对HLA-B27等抗原进行检测,抗核抗体采用间接免疫荧光法,免疫球蛋白及补体均采用免疫速率散射法测定.结果126例初诊患者中HLA-B27阳性率为34.1%,HLA-B7、B13、B40的阳性率分别为6.3%,13.5%,11.1%.HLA-B27阳性组中HLA-B13阳性率为27.9%,HLA-B40阳性率为23.3%,分别高于HLA-B27阴性组(6.0%,4.8%),而HLA-B7的阳性率两组无显著性差异.HLA-B27阳性组的免疫球蛋白、补体、抗核抗体指标均显著高于HLA-B27阴性组(P均<0.05).结论检测强直性脊柱炎患者HLA-B27及相关B抗原有助于疾病诊断和抗原交叉反应的分析,体液免疫异常及自身抗体的出现与HLA-B27密切相关.

  2. Association of IL1R polymorphism with HLA-B27 positive in Iranian patients with ankylosing spondylitis.

    Mahmoudi, M; Amirzargar, A A; Jamshidi, A R; Farhadi, E; Noori, S; Avraee, M; Nazari, B; Nicknam, M H

    2011-12-01

    Ankylosing spondylitis (AS) is one of the most common causes of inflammatory arthritis, with an estimated prevalence of 0.1-0.9%. Genetic factors have been strongly implicated in its aetiology, and heritability as assessed by twin studies has been estimated to be >90%. HLA- B27 is almost essential for inheritance of AS; it is not merely sufficient for explaining the pattern of familial recurrence of the disease. This study's purpose is to investigate the association of ankylosing spondylitis with single-nucleotide polymorphisms (SNPs) in the IL-1 family: IL-1a (-889C/T) rs1800587, IL-1b (-511C/T) rs16944, IL-1b (+3962C/T) rs1143634, IL-1R (Pst-1 1970C/T) rs2234650 and IL-1RA (Mspa-1 11100C/T) rs315952. 99 unrelated Iranian AS patients and 217 healthy control subjects were selected. Cytokine typing was performed by the polymerase chain reaction with sequence-specific primers assay. The allele and genotype frequencies of the polymorphisms were determined: The IL1α rs1800587, IL1β rs16944 and IL1β rs1143634 were not significantly associated with AS. Genotype frequencies at IL1R rs2234650 differed between cases and controls (χ(2)=8.85; p=0.01); the IL1R rs2234650 C/T and T/T genotypes were less common in AS patients than controls. The IL1R rs2234650 C/T genotype was inversely associated with AS comparing with the IL1R rs2234650 C/C genotype (OR=0.48; p=0.005). IL1R rs2234650 C/T genotype was less common in patients than controls (OR=0.37; p=0.02).Furthermore IL1R rs2234650 T allele was strongly associated with HLA-B2702 patients rather than HLA-B2705 but was not associated with HLA-B27 negative patients (OR=0.33; p=0.01). Polymorphisms of IL1α rs1800587, IL1β rs16944 and IL1β rs1143634 were not significantly associated with ankylosing spondylitis but inversely in this study IL1R rs2234650 was significantly associated and carriage of T allele in IL1R rs2234650 seems to be protective, while carriage of C allele result in two fold higher risk of developing AS. PMID

  3. The correlation analysis of HLA-B27 in the diagnosis of ankylosing spondyfitis%人类白细胞抗原-B27与强直性脊柱炎的相关性分析

    朱振军; 程田; 李碧波

    2010-01-01

    目的 探讨人类白细胞抗原-B27(HLA-B27)检测在强直性脊柱炎(AS)早期诊断中的临床价值.方法 使用序列特异性引物-聚合酶链式反应(PCR-SSP)法对500例健康体检者(非AS对照组)、360例AS疑似患者(AS疑似组)和100例AS患者(AS组) 进行HLA-B27抗原检测.结果 非AS对照组、AS疑似组和AS组的HLA-B27阳性率分别为3.3%、 48.9%和92.5%,AS组、AS疑似组的HLA-B27阳性率明显高于非AS对照组(P<0.01),AS组的HLA-B27阳性率明显高于AS疑似组(P<0.01).结论 HLA-B27抗原与AS疾病相关,检测HLA-B27抗原对疑似AS患者的早期诊断有重要意义.%Objective To explore the clinical value of HLA-B27 in patients with ankylosing spondyfitis. Methods Using PCR-SSP to detect the HLA-B27 of 500 healthy people (non-AS group),360 presumed AS patients (suspectable group) and 100 AS patients(AS group). Results The positive rates of HLA-B27 of non-AS group, suspectable group, AS group were 3.3%,48.9% and 92.5%, respectively. The positive rate of HLA-B27 of AS group and suspectable group was obviously higher than that in non-AS group (P<0.05). The positive rate of HLA-B27 of AS group was obviously higher than that in suspectable group (P<0.05). Conclusions There is positive correlation between AS and HLA-B27.It is very important for the early diagnosis of AS.

  4. Sulfasalazine Treatment Suppresses the Formation of HLA-B27 Heavy Chain Homodimer in Patients with Ankylosing Spondylitis

    Hui-Chun Yu

    2015-12-01

    Full Text Available Human leukocytic antigen-B27 heavy chain (HLA-B27 HC has the tendency to fold slowly, in turn gradually forming a homodimer, (B27-HC2 via a disulfide linkage to activate killer cells and T-helper 17 cells and inducing endoplasmic reticulum (ER stress to trigger the IL-23/IL-17 axis for pro-inflammatory reactions. All these consequences lead to the pathogenesis of ankylosing spondylitis (AS. Sulfasalazine (SSA is a common medication used for treatment of patients with AS. However, the effects of SSA treatment on (B27-HC2 formation and on suppression of IL-23/IL-17 axis of AS patients remain to be determined. In the current study, we examine the (B27-HC2 of peripheral blood mononuclear cells (PBMC, the mean grade of sarcoiliitis and lumbar spine Bath Ankylosing Spondylitis Radiology Index (BASRI scores of 23 AS patients. The results indicated that AS patients without (B27-HC2 on PBMC showed the lower levels of mean grade of sarcoiliitis and the lumbar spine BASRI scores. In addition, after treatment with SSA for four months, the levels of (B27-HC2 on PBMCs were significantly reduced. Cytokines mRNA levels, including TNFα, IL-17A, IL-17F and IFNγ, were also significantly down-regulated in PBMCs. However, SSA treatment did not affect the levels of IL-23 and IL-23R mRNAs.

  5. Sulfasalazine Treatment Suppresses the Formation of HLA-B27 Heavy Chain Homodimer in Patients with Ankylosing Spondylitis.

    Yu, Hui-Chun; Lu, Ming-Chi; Huang, Kuang-Yung; Huang, Hsien-Lu; Liu, Su-Qin; Huang, Hsien-Bin; Lai, Ning-Sheng

    2016-01-01

    Human leukocytic antigen-B27 heavy chain (HLA-B27 HC) has the tendency to fold slowly, in turn gradually forming a homodimer, (B27-HC)₂ via a disulfide linkage to activate killer cells and T-helper 17 cells and inducing endoplasmic reticulum (ER) stress to trigger the IL-23/IL-17 axis for pro-inflammatory reactions. All these consequences lead to the pathogenesis of ankylosing spondylitis (AS). Sulfasalazine (SSA) is a common medication used for treatment of patients with AS. However, the effects of SSA treatment on (B27-HC)₂ formation and on suppression of IL-23/IL-17 axis of AS patients remain to be determined. In the current study, we examine the (B27-HC)₂ of peripheral blood mononuclear cells (PBMC), the mean grade of sarcoiliitis and lumbar spine Bath Ankylosing Spondylitis Radiology Index (BASRI) scores of 23 AS patients. The results indicated that AS patients without (B27-HC)₂ on PBMC showed the lower levels of mean grade of sarcoiliitis and the lumbar spine BASRI scores. In addition, after treatment with SSA for four months, the levels of (B27-HC)₂ on PBMCs were significantly reduced. Cytokines mRNA levels, including TNFα, IL-17A, IL-17F and IFNγ, were also significantly down-regulated in PBMCs. However, SSA treatment did not affect the levels of IL-23 and IL-23R mRNAs. PMID:26729099

  6. KIR3DL1 interaction with HLA-B27 is altered by ankylosing spondylitis associated ERAP1 and enhanced by MHC class I cross-linking.

    Abdullah, Hasan; Zhang, Zhenbo; Yee, Kirby; Haroon, Nigil

    2015-01-01

    Ankylosing spondylitis (AS) is a chronic, inflammatory arthritis of the spine and peripheral joints linked to the antigen presenting molecule HLA-B27. The risk of AS is increased in patients possessing endoplasmic reticulum aminopeptidase-1 (ERAP1) polymorphisms rs30187 and rs27044 encoding amino acid changes K528R and Q730E, respectively. Dysfunction of ERAP1 is hypothesized to cause changes in expression of HLA-B27 classical (pHLA) and non-classical (FHC) conformers on antigen presenting cells (APCs), which interact with the natural killer (NK) cell receptor KIR3DL1. Dysregulation of this pathway may be pathogenic in AS. APC cell lines expressing HLA-B27 were found to inhibit cytokine production in KIR3DL1+ NK cells due to decreased APC-NK cell adhesion, and possibly activation of receptor down-regulation. Blocking pHLA and FHC reveals that both conformers inhibit cytokine production through KIR3DL1. KIR3DL1 affinity and HLA-B27 surface expression studies suggest that ERAP1 R528 and E730 expression protects from AS by generating sub-optimal pHLA, causing reduced KIR3DL1 affinity and weaker cytokine inhibition. Secondarily we observed that KIR3DL1 binding to C1R-B27 APCs is enhanced by blocking pHLA, but not FHC, raising the possibility that antibody mediated HLA-B27 cross-linking may be important in enhancing KIR3DL1+ NK cell function. This study establishes the role of both FHC and pHLA in modulating NK cell cytokine secretion and adhesion functions by interacting with KIR3DL1. This interaction varies depending on the AS association status of the ERAP1 variant expressed in APCs. Additionally antibody cross-linking of HLA-B27 enhances KIR3DL1 binding and as such could be an important pathogenic mechanism in AS. PMID:26321090

  7. 强直性脊柱炎患者HLA-B27、B7、B13和B40的检测及其与临床相关性的研究

    潘静芳; 黄瑛

    2005-01-01

    目的探讨湖北省强直性脊柱炎 (AS)患者 HLA- B27、 B7、 B13和 B40的抗原表达情况及其临床诊断意义.方法采用微量淋巴细胞毒法检测 179例 AS患者 HLA- B27、 B7、 B13和 B40的抗原表达情况,并分析其与临床的相关性.结果 HLA- B27、 B7、 B13和 B40的抗原阳性率分别为 74.9%、 11.7%、 14.0%和 13.4%.HLA- B27阳性的 AS患者多以外周关节受累为主要症状,其平均发病年龄比 HLA- B27阴性的 AS患者小; HLA- B27阴性的 AS患者多以腰痛为主要症状,其平均发病年龄较大.结论 HLA- B27阳性对以外周关节受累为首发症状且年龄在 25岁以下的 AS患者诊断意义较大.

  8. Association between HLA-B27 antigen and acute anterior uveitis%人类白细胞抗原与急性前葡萄膜炎的相关性

    杜红艳; 杜鹏程

    2009-01-01

    目的:探讨急性前葡萄膜炎(acute anterior uveitis,AAU)与人类白细胞抗原(human leucolyte antigen,HLA-B27)的相关性.方法:应用聚合酶链反应(sequence special prime polymerase chain reaction,SSP-PCR)基因检测技术,对AAU患者进行HLA-B27基因检测,观察HLA-B27阳性与阴性AAU临床特征并加以分析.结果:在36例AAU患者中,HLA-B27抗原阳性29例,阳性率81%,37例健康对照组,阳性9例,阳性率24%,两者有显著性差异(χ2=23.12,P<0.01).结论:HLA-B27与AAU明显相关,HLA-B27阳性与阴性患者有一定程度的差异,常伴有全身脊柱关节病,采用SSP-PCR基因检测技术测定HLA-B27快速、简单、准确性高、客观性强,值得推广应用.

  9. HLA-B27检测在强直性脊柱炎诊断中的临床价值探讨

    刘晖

    2010-01-01

    目的探讨HLA—B27检测在强直性脊柱炎(AS)诊断中的临床价值。方法选择我院确诊的AS患者83例作为观察组,随机抽取在我院健康体检人群80例做为对照组,进行HLA~B27检测。结果观察组HLA—B27阳性率明显高于健康对照组,差异有统计学意义(P〈0.01)。结论HLA-B27与AS具有很强的相关性,检测HLA-B27可明显提高诊断特异性。

  10. Türk ankilozan spondilitli hastalarda HLA-B27 ve MEFV gen mutasyonları arasındaki ilişki

    SARIKAYA PEKACAR, Filiz

    2015-01-01

    Ankilozan spondilit (AS), patogenezinde bir çok genetik, immunolojik ve çevresel faktörün birlikte rol oynadığı  inflamatuvar bir hastalıktır. Genetik faktörler arasında en büyük pay HLA -B27’nindir. Genom  çapında ilişkilendirme çalışmaları sonucu AS ile ilgili bir çok gen bulunmuş MHC dışı genlerin hastalığın  gelişiminde rol oynadığı düşünülmüştür.
    Bu tez çalışmasında HLA-B27, MEFV gen mutasyonları, IL12B, IL23R ve ERAP1 gen polimorfiz...

  11. Loss of viral fitness and cross-recognition by CD8+ T cells limit HCV escape from a protective HLA-B27–restricted human immune response

    Dazert, Eva; Neumann-Haefelin, Christoph; Bressanelli, Stéphane; Fitzmaurice, Karen; Kort, Julia; Timm, Jörg; McKiernan, Susan; Kelleher, Dermot; Gruener, Norbert; Tavis, John E.; Rosen, Hugo R.; Shaw, Jaqueline; Bowness, Paul; Blum, Hubert E.; Klenerman, Paul

    2009-01-01

    There is an association between expression of the MHC class I molecule HLA-B27 and protection following human infection with either HIV or HCV. In both cases, protection has been linked to HLA-B27 presentation of a single immunodominant viral peptide epitope to CD8+ T cells. If HIV mutates the HLA-B27–binding anchor of this epitope to escape the protective immune response, the result is a less-fit virus that requires additional compensatory clustered mutations. Here, we sought to determine wh...

  12. The Rate of Helicobacter pylori Seropositivity in a Group of Korean Patients with HLA-B27-Associated Acute Anterior Uveitis

    Jeong Hun Bae; Joon Mo Kim

    2015-01-01

    Purpose To investigate an association between Helicobacter pylori seropositivity and HLA-B27-positive acute anterior uveitis (AAU) in Korean patients. Methods Retrospective analysis was performed with data from 106 patients previously diagnosed with AAU without clinical evidence of spondyloarthropathy. Serum immunoglobulin G antibodies to H. pylori were measured by enzyme-linked immunosorbent assay, and HLA typing was performed using polymerase chain reaction of DNA amplification. We included...

  13. AIDS-protective HLA-B*27/B*57 and chimpanzee MHC class I molecules target analogous conserved areas of HIV-1/SIVcpz

    de Groot, Natasja G.; Corrine M C Heijmans; Zoet, Yvonne M.; de Ru, Arnoud H.; Verreck, Frank A.; van Veelen, Peter A.; Drijfhout, Jan W; Doxiadis, Gaby G M; Remarque, Edmond J.; Doxiadis, Ilias I. N.; van Rood, Jon J.; Koning, Frits; Bontrop, Ronald E

    2010-01-01

    In the absence of treatment, most HIV-1-infected humans develop AIDS. However, a minority are long-term nonprogressors, and resistance is associated with the presence of particular HLA-B*27/B*57 molecules. In contrast, most HIV-1-infected chimpanzees do not contract AIDS. In comparison with humans, chimpanzees experienced an ancient selective sweep affecting the MHC class I repertoire. We have determined the peptide-binding properties of frequent chimpanzee MHC class I molecules, and show tha...

  14. Genetic Polymorphisms of Stromal Interaction Molecule 1 Associated with the Erythrocyte Sedimentation Rate and C-Reactive Protein in HLA-B27 Positive Ankylosing Spondylitis Patients

    Wei, James Cheng-Chung; Hung, Kuo-Sheng; Hsu, Yu-Wen; Wong, Ruey-Hong; Huang, Chun-Huang; Jan, Ming-Shiou; Wu, Shyh-Jong; Juan, Yung-Shun; Chang, Wei-Chiao

    2012-01-01

    Ankylosing spondylitis (AS) is a chronic inflammation of the sacroiliac joints, spine and peripheral joints. The development of ankylosing spondylitis is still unclear. Genetics factors such as human leukocyte antigen HLA-B27 and ERAP1 have been widely reported to associate to AS susceptibility. In this study, we enrolled 361 AS patients and selected four tagging single nucleotides polymorphisms (tSNPs) at STIM1 gene. The correlation between STIM1 genetic polymorphisms and AS activity index (...

  15. Doğu Anadolu Bölgesinde Ankilozan Spondilitli Hastalarda HLA B27 Frekansının Dağılımı

    Diyarbakır, Eda; Eyerci, Nilnur; UZKESER, Hülya; Karatay, Saliha; Topçu, Atilla; Yıldırım, Kadir; Pirim, İbrahim

    2012-01-01

    Ankilozan spondilit (AS) etyolojisi bilinmeyen kronik enflamatuvar bir hastalıktır. Patogenezde genetik ve çevresel faktörlerin önemli rol oynadığı bilinmektedir. AS gelişimi ile HLA B27 arasında güçlü bir birliktelik saptanmıştır. Bu çalışma ile daha önceki literatür bilgileri ışığında bölgemizdeki HLA B27-AS ilişkisi karşılaştırıldı. Bu çalışmada, modifiye New York kritelerine göre sınıflandırılmış AS'li hasta ve sağlıklı kontroller üzerinde HLA B27 frekansı araştırılmıştır. Çalışmaya b...

  16. A computational docking study on the pH dependence of peptide binding to HLA-B27 sub-types differentially associated with ankylosing spondylitis.

    Serçinoğlu, Onur; Özcan, Gülin; Kabaş, Zeynep Kutlu; Ozbek, Pemra

    2016-07-01

    A single amino acid difference (Asp116His), having a key role in a pathogenesis pathway, distinguishes HLA-B*27:05 and HLA-B*27:09 sub-types as associated and non-associated with ankylosing spondylitis, respectively. In this study, molecular docking simulations were carried out with the aim of comprehending the differences in the binding behavior of both alleles at varying pH conditions. A library of modeled peptides was formed upon single point mutations aiming to address the effect of 20 naturally occurring amino acids at the binding core peptide positions. For both alleles, computational docking was applied using Autodock 4.2. Obtained free energies of binding (FEB) were compared within the peptide library and between the alleles at varying pH conditions. The amino acid preferences of each position were studied enlightening the role of each on binding. The preferred amino acids for each position of pVIPR were found to be harmonious with experimental studies. Our results indicate that, as the pH is lowered, the capacity of HLA-B*27:05 to bind peptides in the library is largely lost. Hydrogen bonding analysis suggests that the interaction between the main anchor positions of pVIPR and their respective binding pocket residues are affected from the pH the most, causing an overall shift in the FEB profiles. PMID:27506766

  17. 强直性脊柱炎患者HLA-B27、B7、B13、B40抗原的表达及免疫学指标分析

    李士军; 王滨; 曹华军

    2002-01-01

    [目的]探讨强直性脊柱炎患者HLA-B27、B7、B13、B40的抗原表达,发现抗原相互杂合与交叉反应,以及强直性脊柱炎患者的免疫学指标的变化。[方法]采用NIH微量淋巴细胞毒法对HLA-B27等抗原进行检测,抗核抗体采用间接免疫荧光法。免疫球蛋白及补体均采用免疫速率散射法测定。[结果]126例初诊患者中HLA-B27阳性率为34.1%,HLA-B27、B13、B40的阳性率分别为6.3%、13.5%、11.1%。HLA-B27阳性组中HLA-B13阳性率为27.9%,HLA-B40阳性率为23.3%,分别高于HLA-B27阴性组(6.0%,4.8%),而HLA-B27的阳性率两组无显著性差异。HLA-B27阳性组的免疫球蛋白、补体、抗核抗体指标均显著高于HLA-B27阴性组(P均<0.05)。[结论]检测强直性脊柱炎患者HLA-B27及相关B抗原有助于疾病诊断和抗原交叉反应的分析。体液免疫异常及自身抗体的出现与HLA-B27密切相关。

  18. Superior oblique tendon (Brown’s syndrome as the presenting finding in childhood onset HLA-B27-related enthesitis and juvenile idiopathic oligoarticular arthritis

    C. Pham

    2014-11-01

    Full Text Available We report two patients who presented with Brown’s syndrome. The first is a 7-year-old boy who at the time of his diagnosis was also found to have enthesitis and HLA-B27 positivity. The second patient was diagnosed with bilateral Brown’s syndrome at 13 months of age. At age 7 she developed a persistent oligoarticular arthritis and unilateral anterior iritis consistent with the oligoarticular Juvenile Idiopatic Arthritis (JIA phenotype. These cases highlight ophthalmologic findings and diagnostic considerations with respect to Brown’s syndrome and associated childhood onset rheumatologic disease.

  19. Cartilage collagen type II seromarker patterns in axial spondyloarthritis and psoriatic arthritis: associations with disease activity, smoking and HLA-B27.

    Munk, Heidi Lausten; Gudmann, Natasja Staehr; Christensen, Anne Friesgaard; Ejstrup, Leif; Sorensen, Grith Lykke; Loft, Anne Gitte; Bay-Jensen, Anne C; Siebuhr, Anne Sofie; Junker, Peter

    2016-04-01

    The aim of the study was to assess the possible association between type II collagen turnover seromarkers and disease profile in patients with axial spondyloarthritis (SpA) and psoriatic arthritis (PsA). Outpatients with axial SpA (n = 110) or PsA (n = 101) underwent clinical examination including disease activity measures and HLA-B27 typing. The procollagen IIA N-terminal peptide (PIIANP) and a matrix metalloproteinase-generated type II collagen fragment (C2M) were quantified in serum by ELISA. C2M was higher in SpA than in controls, 0.41 versus 0.36 ng/ml (p = 0.004), while PIIANP did not differ between patients and healthy subjects, 2252 versus 2142 ng/ml (p = 0.13). However, DMARD-naïve SpA patients had higher PIIANP, 2461 ng/ml (p = 0.01) and C2M, 0.44 ng/ml (p = 0.0007) levels than controls, and PIIANP correlated with CRP (ρ = 0.34). C2M was lower in SpA smokers, 0.36 ng/ml versus non-smokers, 0.43 ng/ml (p = 0.02), while PIIANP was higher in HLA-B27 positive, 2312 ng/ml versus negative patients, 2021 ng/ml (p = 0.03). In PsA, PIIANP and C2M did not differ between patients and controls, but PIIANP was elevated in patients not receiving DMARDs, 2726 ng/ml. In PsA, PIIANP and C2M did not differ according to smoking and HLA-B27. Cartilage degradation assessed by C2M is increased in SpA irrespective of treatment but not in PsA. Cartilage synthesis reflected by PIIANP is increased in untreated SpA and PsA. PIIANP correlates with CRP in SpA while not in PsA. In DMARD-naïve SpA but not in PsA, HLA-B27 positivity and smoking are associated with a chondro-proliferative metabolic pattern. PMID:26620690

  20. Superior oblique tendon (Brown’s) syndrome as the presenting finding in childhood onset HLA-B27-related enthesitis and juvenile idiopathic oligoarticular arthritis

    Pham, C; V. Utz; A. Marcotty; A. Zeft; P. Rychwalski

    2014-01-01

    We report two patients who presented with Brown’s syndrome. The first is a 7-year-old boy who at the time of his diagnosis was also found to have enthesitis and HLA-B27 positivity. The second patient was diagnosed with bilateral Brown’s syndrome at 13 months of age. At age 7 she developed a persistent oligoarticular arthritis and unilateral anterior iritis consistent with the oligoarticular Juvenile Idiopatic Arthritis (JIA) phenotype. These cases highlight ophthalmologic findings and diagnos...

  1. Endoplasmic reticulum aminopeptidase-1 alleles associated with increased risk of Ankylosing Spondylitis reduce HLA-B27 mediated presentation of multiple antigens

    Seregin, Sergey S.; Rastall, David P. W.; Evnouchidou, Irini; Charles F Aylsworth; Quiroga, Dionisia; Kamal, Ram P.; Godbehere-Roosa, Sarah; Blum, Christopher F.; Ian A York; Stratikos, Efstratios; Amalfitano, Andrea

    2013-01-01

    Ankylosing spondylitis (AS) is a chronic systemic arthritic disease that leads to significant disability and loss of quality of life in the ~0.5% of the worldwide human population it affects. There is currently no cure for AS and mechanisms underlying its pathogenesis remain unclear. AS is highly genetic, with over 70% of the genetic risk being associated with the presence of HLA-B27 and endoplasmic reticulum aminopeptidase-1 (ERAP1) alleles. Furthermore, gene-gene interactions between HLA-B2...

  2. An HLA-C amino-acid variant in addition to HLA-B*27 confers risk for ankylosing spondylitis in the Korean population

    Kim, Kwangwoo; Bang, So-Young; Lee, Seunghun; Lee, Hye-Soon; Shim, Seung-Cheol; Kang, Young Mo; Suh, Chang-Hee; Sun, Celi; Nath, Swapan K; Bae, Sang-Cheol; Kim, Tae-Hwan

    2015-01-01

    Introduction The presence of the HLA-B*27 allele is a major risk factor for the development of ankylosing spondylitis (AS), which causes chronic inflammation of the spine and other sites. We investigated residual effects outside HLA-B within the major histocompatibility complex (MHC) region in the Korean population. Methods Using the Korean HLA reference panel, we inferred the classic HLA alleles and amino-acid residues of the six HLA genes (HLA-A, -B, -C, -DPB1, -DQB1, and -DRB1) and MHC sin...

  3. 人白细胞抗原-B27在诊断强直性脊柱炎中的意义%Clinical significance of HLA-B27 test in diagnosis of ankylosing spondylitis

    陈国敏; 王东辰

    2007-01-01

    目的 探讨人白细胞抗原-B27(HLA-B27)检测诊断强直性脊柱炎(AS)的意义.方法 应用ELISA法,对107例AS患者、70例类风湿性关节炎(RA)患者及100例健康体检者进行HLA-B27对比检测.结果 AS组HLA-B27阳性率为92.5%,RA组阳性率为7.1%,体检组阳性率为3.0%,AS组明显高于RA组和体检对照组,差异有统计学意义(χ2=214,P<0.01).男性HLA-B27阳性率(49.7%)明显高于女性(14.8%),差异有统计学意义(χ2=30.9,P<0.01).不同年龄组HLA-B27阳性率有明显差异,30岁以下年龄组明显高于其他年龄组.结论 HLA-B27与AS具有很强的相关性,检测HLA-B27对于AS的早期诊断和鉴别具有重要意义.

  4. EVOLUCIÓN DE ARTRITIS PSORIÁSICA A PARTIR DE ESPONDILOARTROPATÍA INDIFERENCIADA NO ASOCIADA AL ANTIGENO HLA-B27: REPORTE DE UN CASO

    Diego Bernardo Alarcón Granados

    2016-07-01

    Full Text Available Paciente masculino, 56 años de edad, cuya enfermedad actual inicia a los 21 años de edad, presentando lumbalgia inflamatoria. Concomitantemente monoartritis en rodilla izquierda, de aparición brusca, limitando su deambulación. Acude a especialista quien evalúa y diagnostica espondiloartropatía indiferenciada. Meses después, reaparecen episodios de lumbalgia inflamatoria por lo que procede a automedicarse. 27 años después presenta prurito en cuero cabelludo y dorso de antebrazo, así como placas eritematosas, descamativas, de tamaño variable, bordes delimitados. Acude a dermatólogo quien diagnostica psoriasis. Meses después, aparecen lesiones de psoriasis generalizadas, oligoartritis y lumbalgia mecánica. Es reevaluado diagnosticándose artritis psoriásica. Actualmente, presenta perdida de la lordosis lumbar, sindesmofitos y sacroileítis bilateral como hallazgos radiológicos. La población afectada por artritis psoriásica oscila entre 0,02 al 0,42%; en un 17% de los casos la artritis precede a la psoriasis. Los casos con espondilitis se asocian a sacroileítis y HLA-B27 positivo; sólo 10% de las espondilitis presenta HLA-B27 negativo. Palabras Clave: Artritis Psoriásica, espondiloartropatía, psoriasis.

  5. Genetic polymorphisms of stromal interaction molecule 1 associated with the erythrocyte sedimentation rate and C-reactive protein in HLA-B27 positive ankylosing spondylitis patients.

    James Cheng-Chung Wei

    Full Text Available Ankylosing spondylitis (AS is a chronic inflammation of the sacroiliac joints, spine and peripheral joints. The development of ankylosing spondylitis is still unclear. Genetics factors such as human leukocyte antigen HLA-B27 and ERAP1 have been widely reported to associate to AS susceptibility. In this study, we enrolled 361 AS patients and selected four tagging single nucleotides polymorphisms (tSNPs at STIM1 gene. The correlation between STIM1 genetic polymorphisms and AS activity index (BASDAI, BASFI, BAS-G as well as laboratory parameters of inflammation (erythrocyte sedimentation rate (ESR and C-reactive protein (CRP were tested. Our results indicated that HLA-B27 positive AS patients who are carrying the minor allele homozygous G/G genotype of SNP rs3750996 significantly associated with a higher level of ESR in serum. Furthermore, rs3750996/rs3750994 pairwise allele analysis indicated that G-C haplotypes also significantly correlated with higher level of ESR as well as CRP. These findings provide a better understanding of STIM1 genetic contribution to the pathogenesis of AS.

  6. In vivo functions of CPSF6 for HIV-1 as revealed by HIV-1 capsid evolution in HLA-B27-positive subjects.

    Matthew S Henning

    2014-01-01

    Full Text Available The host protein CPSF6 possesses a domain that can interact with the HIV-1 capsid (CA protein. CPSF6 has been implicated in regulating HIV-1 nuclear entry. However, its functional significance for HIV-1 replication has yet to be firmly established. Here we provide evidence for two divergent functions of CPSF6 for HIV-1 replication in vivo. We demonstrate that endogenous CPSF6 exerts an inhibitory effect on naturally occurring HIV-1 variants in individuals carrying the HLA-B27 allele. Conversely, we find a strong selective pressure in these individuals to preserve CPSF6 binding, while escaping from the restrictive activity by CPSF6. This active maintenance of CPSF6 binding during HIV-1 CA evolution in vivo contrasts with the in vitro viral evolution, which can reduce CPSF6 binding to evade from CPSF6-mediated restriction. Thus, these observations argue for a beneficial role of CPSF6 for HIV-1 in vivo. CPSF6-mediated restriction renders HIV-1 less dependent or independent from TNPO3, RanBP2 and Nup153, host factors implicated in HIV-1 nuclear entry. However, viral evolution that maintains CPSF6 binding in HLA-B27+ subjects invariably restores the ability to utilize these host factors, which may be the major selective pressure for CPSF6 binding in vivo. Our study uncovers two opposing CA-dependent functions of CPSF6 in HIV-1 replication in vivo; however, the benefit for binding CPSF6 appears to outweigh the cost, providing support for a vital function of CPSF6 during HIV-1 replication in vivo.

  7. 强直性脊柱炎患者HLA-B27抗原表达与骨损害关系的临床研究%Clinical study of relationship between HLA-B27 antigen expression and bone damage in patients of ankylosing spondylitis

    王丽; 陈程; 杨忠礼

    2005-01-01

    目的探讨HLA-B27表达与强直性脊柱炎患者骨代谢功能损伤的关系.方法检测HLA-B27不同型别的强直性脊柱炎患者和对照群体骨代谢功能指标.结果研究显示各组间各项指标存在着不同程度的统计学差异,具体表现为:CK检测水平B27+AS>B27-AS和B27-对照(P<0.05),而B27+AS和B27-对照之间无显著差异(P>0.05);AKP检测水平B27+AS>B27-AS>B27+对照和B27-对照(P<0.05),而B27+对照和B27对照之间无显著差异(P>0.05);CT检测水平B27+AS>B27-AS>B27+对照>B27-对照(P<0.05):BGP检测水平B27+AS>B27-AS>B27+对照>B27-对照(P<0.01).结论强直性脊柱炎患者HLA-B27的表达与骨质损害具有一定的关联性,为临床深入研究提供了有益的线索.

  8. Genetic markers as a predictive tool based on statistics in medical practice: ethical considerations through the analysis of the use of HLA-B27 in rheumatology in France

    Hélène eColineaux

    2015-10-01

    Full Text Available INTRODUCTION. The use of genetic predictive markers in medical practice does not necessarily bear the same kind of medical and ethical consequences than that of genes directly involved in monogenic diseases. However, the French bioethics law framed in the same way the production and use of any genetic information. It seems therefore necessary to explore the practical and ethical context of the actual use of predictive markers in order to highlight their specific stakes. In this study, we document the uses of HLA-B*27, which are an interesting example of the multiple features of genetic predictive marker in general medical practice.MATERIAL & METHODS. The aims of this monocentric and qualitative study were to identify concrete and ethical issues of using the HLA-B*27 marker and the interests and limits of the legal framework as perceived by prescribers. In this regard, a thematic and descriptive analysis of five rheumatologists’ semi-structured and face-to-face interviews was performed.RESULTS. According to most of the interviewees, HLA-B*27 is an overframed test because they considered that this test is not really genetic or at least does not have the same nature as classical genetic tests; HLA-B*27 is not concerned by the ethical challenges of genetic test; the major ethics stake of this marker is not linked to its genetic nature but rather to the complexity of the probabilistic information. This study allows also showing that HLA-B*27, validated for a certain usage, may be used in different ways in practice.DISCUSSION. This marker and its clinical uses underline the challenges of translating both statistical concepts and unifying legal framework in clinical practice. This study allows identifying some new aspects and stakes of genetics in medicine and shows the need of additional studies about the use of predictive genetic markers, in order to provide a better basis for decisions and legal framework regarding these practices.

  9. HLA-B27基因亚型、KIR基因和HLA-Cw基因与强直性脊柱炎病情活动的关系%The Relationship of the Subtypes of HLA-B27,HLA-Cw and KIRs with the Activity of Ankylosing Spondylitis

    张谷香; 赵福涛

    2016-01-01

    Objective To study the association of the polymorphism of human leukocyte antigen-B27 (HLA-B27),Killer immunoglobulin receptor(KIR),and human leukocyte antigen-Cw(HLA-Cw) genes with the activity of ankylosing spondylitis(AS).Methods Total of 25 patients with active AS admitted to Shanghai Third People′s Hospital Affiliated to Shanghai Jiaotong University from Jun .2011 to Mar.2013 were selected as trial group,41 patients with stable AS were inlcuded as control group.HLA-B27 subtype,KIR gene and HLA-Cw gene were detected by polymerase chain reaction with with sequence-specific primers (PCR-SSP).Results The gene frequency of HLA-Cw02,HLA-Cwlys and KIR3DS1 in the trial group were significantly higher than those in the control group[44.0%(11/25)vs 15.4%(6/41),48.0%(12/25) vs 22.0%(9/41),68.0%(17/25) vs 41.5%(17/41)],the differences were statistically significant(P <0.05).Conclusion The dramatic increase of frequencies of KIR3DS1,HLA-Cw02 and HLA-Cwlys in the active AS patients indicates KIRs gene and HLA-Cw gene is associated with the activity of AS .%目的 分析人类白细胞抗原( HLA)-B27 基因多态性、杀伤细胞免疫球蛋白样受体( KIR)基因和HLA-Cw基因与强直性脊柱炎( AS)病情活动的关系. 方法 选择2011 年6 月至2013 年3月上海交通大学医学院附属第三人民医院收治的活动期 AS患者25 例作为试验组,稳定期 AS患者41例作为对照组,采用特异性引物聚合酶链反应( PCR-SSP )技术进行 HLA-B27 亚型、KIR 基因、HLA-Cw基因检测. 结果 试验组 HLA-Cw02 和 HLA-Cwlys、 KIR3DS1 的基因频率显著高于对照组[44.0%(11/25)比15.4%(6/41),48.0%(12/25)比22.0%(9/41),68.0%(17/25)比41.5%(17/41)],差异有统计学意义(P<0.05). 结论 HLA-Cw02 和 HLA-Cwlys、KIR3DS1 基因频率在活动期AS患者中明显增高,表明KIRs基因和HLA-Cw基因与AS的病情活动有关.

  10. The study between the gene and the subtypes of HLA-1327 and ankylosing spondylitis in Hainan%海南地区HLA-B27及其亚型与强直性脊椎炎相关性研究

    徐卫华; 符生苗; 赵英

    2008-01-01

    目的 通过检测海南地区20个AS患者家族的HLA-B27基因,以获得HLA-B27抗原以及基因亚型的分布情况.方法 B27抗原检测和基因亚型检测均采用序列特异性引物聚合酶链式反应(PCR-SSR)技术.结果 B27阳性者患AS的概率为57.7%,其中男女比例约为3.6:1,AS患者中HLA-B27阳性者占93.2%,阳性与阴性的患病比例高达14:1.AS患者一级亲属HLA-B27阳性率52.7%,患病率21.6%,HLA-B27阳性者其后代仍为阳性的概率55.4%;基因分型均为HLA-B2704亚型.结论 海南地区HLA-B27阳性和As发病率均与性别有关,并具有家族遗传性,HLA-B27基因亚型为132704.