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1

Bacteriophage P22 capsid size determination: Roles for the coat protein telokin-like domain and the scaffolding protein amino-terminus  

British Library Electronic Table of Contents (United Kingdom)

Assembly of icosahedral capsids of proper size and symmetry is not understood. Residue F170 in bacteriophage P22 coat protein is critical for conformational switching during assembly. Substitutions at this site cause assembly of tubes of hexamerically arranged coat protein. Intragenic suppressors of the ts phenotype of F170A and F170K coat protein mutants were isolated. Suppressors were repeatedly found in the coat protein telokin-like domain at position 285, which caused coat protein to assemble into petite procapsids and capsids. Petite capsid assembly strongly correlated to the side chain volume of the substituted amino acid. We hypothesize that larger side chains at position 285 torque the telokin-like domain, changing flexibility of the subunit and intercapsomer contacts. Thus, a sing...

2011-01-01

2

Structural aberrations in group A Staphylococcus bacteriophages.  

UK PubMed Central (United Kingdom)

Six related Staphylococcus phages spontaneously produced various abnormal head and tail structures: (i) giant capsids which were tailed and apparently contained nucleic acid; (ii) regular and irregular...Full Text Available

1976-05-01

3

Genetic Control of Capsid Length in Bacteriophage T4 I. Isolation and Preliminary Description of Four New Mutants  

UK PubMed Central (United Kingdom)

Four new mutants are described whose phenotypic expression affects the length of the head of bacteriophage T4D. All mutants produce some phenotypically normal phage particles. Mutant pt21-34...Full Text Available

1973-08-01

4

Interaction between Bluetongue virus outer capsid protein VP2 and vimentin is necessary for virus egress  

UK PubMed Central (United Kingdom)

BackgroundThe VP2 outer capsid protein Bluetongue Virus (BTV) is responsible for receptor binding, haemagglutination and eliciting host-specific immunity. However, the assembly of...Full Text Available

7

Silicates as Nonspecific Adsorbents of Bacteriophage: a Model for Purification of Water from Viruses  

UK PubMed Central (United Kingdom)

Amorphous silicates having small particles with a large surface area were found to have high nonspecific adsorption capacity. Investigations with Escherichia coli T4 bacteriophage have...Full Text Available

1980-01-01

8

Organization of Bacteriophage Tail-Like Particles in Cells of Chromobacterium violaceum  

UK PubMed Central (United Kingdom)

A strain of Chromobacterium violaceum has been isolated which produces bacteriophage tail-like particles in high numbers. The extracellular morphology and the intracellular arrangement...Full Text Available

1972-05-01

9

Mutational analysis of bacteriophage lambda lysis gene S.  

UK PubMed Central (United Kingdom)

A plasmid carrying the bacteriophage lambda lysis genes under lac control was subjected to hydroxylamine mutagenesis, and mutations eliminating the host lethality of the S gene were selected. DNA sequence...Full Text Available

1986-09-01

10

Gyrase B Inhibitor Impairs HIV-1 Replication by Targeting Hsp90 and the Capsid Protein*  

UK PubMed Central (United Kingdom)

Chemical genetics is an emerging approach to investigate the biology of host-pathogen interactions. We screened several inhibitors of ATP-dependent DNA motors and detected the gyrase B inhibitor coumermycin...Full Text Available

2010-12-10

11

Isolation of a bacterial host selective for bacteriophage T4 containing cytosine in its DNA.  

UK PubMed Central (United Kingdom)

An Escherichia coli B strain, B834 galU56, has been isolated which supports growth of bacteriophage T4 with cytosine in its DNA while restricting growth of T4 with hydroxymethylcytosine. This host is...Full Text Available

1978-09-01

12

Isolation and sequence determination of 5'-terminal oligonucleotide fragments of RNA transcripts synthesized by bacteriophage T3-induced RNA polymerase from T3 DNA.  

UK PubMed Central (United Kingdom)

The nucleotide sequence of the 5'-terminal oligonucleotides produced by pancreatic RNase digestion of bacteriophage T3 RNA polymerase (EC 2.7.7.6) transcripts of T3 DNA has been determined. The sequence...Full Text Available

1980-07-01

13

Experimental Differential Light-Scattering Correction to the Circular Dichroism of Bacteriophage T2  

UK PubMed Central (United Kingdom)

Experimental techniques are presented that can be used to assay and correct for differential light scattering effects in circular dichroism spectra of biological macrostructures. The assay is based...Full Text Available

1973-01-01

14

Characterization of T-Even Bacteriophage Substructures  

UK PubMed Central (United Kingdom)

Tail plates obtained from T4D amber mutants were examined with respect to sedimentation behavior, subunit molecular weights, amino acid composition, isoelectric points, and morphology. Intact plates...Full Text Available

1970-10-01

15

Long-term Retinal Function and Structure Rescue Using Capsid Mutant AAV8 Vector in the rd10 Mouse, a Model of Recessive Retinitis Pigmentosa  

British Library Electronic Table of Contents (United Kingdom)

The retinal degeneration 10 (rd10) mouse is a well-characterized model of autosomal recessive retinitis pigmentosa (RP), which carries a spontaneous mutation in the ? subunit of rod cGMP-phosphodiesterase (PDE?). Rd10 mouse exhibits photoreceptor dysfunction and rapid rod photoreceptor degeneration followed by cone degeneration and remodeling of the inner retina. Here, we evaluate whether gene replacement using the fast-acting tyrosine-capsid mutant AAV8 (Y733F) can provide long-term therapy in this model. AAV8 (Y733F)-smCBA-PDE? was subretinally delivered to postnatal day 14 (P14) rd10 mice in one eye only. Six months after injection, spectral domain optical coherence tomography (SD-OCT), electroretinogram (ERG), optomotor behavior tests, and immunohistochemistry showed tha...

2011-01-01

16

Termination of transcription by bacteriophage T3 RNA polymerase: homogeneous 3'-terminal oligonucleotide sequence of in vitro T3 RNA polymerase transcripts.  

UK PubMed Central (United Kingdom)

RNA was synthesized in vitro from a T3 DNA template by T3 RNA polymerase and subsequently separated into seven discrete size classes (molecular weights ranging between 0.21 x 10(6) and 6.2 x 10(6))...Full Text Available

1979-10-01

17

Model system using coliphage phi X174 for testing virus removal by air filters.  

UK PubMed Central (United Kingdom)

Short-term (15-min-duration) and long-term (5- to 6-day-duration) test procedures have been developed for determining the efficiency of the removal of bacteriophage phi X174 by air-sterilizing filters....Full Text Available

1992-03-01

18

High-cell Density Shake-flask Expression and Rapid Purification of the Large Fragment of Thermus aquaticus DNA Polymerase I Using a New Chemically and Temperature Inducible Expression Plasmid in Escherichia coli*  

UK PubMed Central (United Kingdom)

We have developed a new expression vector, pcIts ind+, based upon the powerful rightward promoter of bacteriophage lambda, which is controlled by a temperature-sensitive...Full Text Available

2009-02-01

19

Pore properties and pharmacological features of the P2X receptor channel in airway ciliated cells  

UK PubMed Central (United Kingdom)

Airway ciliated cells express an ATP-gated P2X receptor channel of unknown subunit composition (P2Xcilia) which is modulated by Na+ and by long exposures to ATP. P2Xcilia...Full Text Available

2006-03-15

 
 
 
 
21

New insights into purinergic receptor signaling in neuronal differentiation, neuroprotection, and brain disorders  

UK PubMed Central (United Kingdom)

Ionotropic P2X and metabotropic P2Y purinergic receptors are expressed in the central nervous system and participate in the synaptic process particularly associated with acetylcholine, GABA, and glutamate...Full Text Available

2007-09-01

23

Structure of an accelerator island with one step on double period of conserving area mapping  

International Nuclear Information System (INIS)

The structure of the P-2, S-1 accelerator mode with one step, S-1, on double period, P-2 for systems, described by the Harper mapping as well as by standard mapping, is analyzed. Detailed analysis of stability criteria is given and comparison with the results of numerical analysis is performed. Three-period compression of the P-2, S-1 island is revealed in the standard mapping. Refs. 7, figs. 7.

24

Purinergic receptors are involved in tooth-pulp evoked nocifensive behavior and brainstem neuronal activity  

UK PubMed Central (United Kingdom)

BackgroundTo evaluate whether P2X receptors are involved in responses to noxious pulp stimulation, the P2X3 and P2X2/3 receptor agonist α,β-methyleneATP...Full Text Available

25

Interaction studies between Crofer-22APU alloy and P2O5 containing barium calcium alumino-borosilicate (BCABS) sealant glass-ceramics  

International Nuclear Information System (INIS)

We present the effect of P2O5 addition on barium calcium aluminum borosilicate BCABS glasses of composition (mol %) 35BaO-15CaO-5Al2O3-(37-x)SiO2-8B2O3-xP2O5 (0?x?5). The incorporation of P2O5 increased network polymerization and crystallization tendency. However, addition of P2O5 leads to the formation of Cr2O3 at the interface, saturating it in the ions of the metal. This improves glass-to-metal bonding. (author)

2010-09-01

26

np-nh bands in the N=28 isotones  

CERN Document Server

The existence of n-particle n-hole deformed yrare bands in the N=28 isotones is explored using full pf-shell diagonalizations and the Lanczos Strength Function method. We find different 2p-2h and 4p-4h collective bands that, when allowed to mix, more often disappear. Only the 2p-2h yrare band in Cr-52 and the 4p-4h yrare band in Ni-56 survive, and only in this latter case, due to the reduced density of 2p-2h states, can the band be seen as a gamma-cascade.

2002-01-01

27

this issue NASA Cloud Computing Platform: Nebula  

Science.gov (United States)

National Aeronautics and Space Administration. Issue 2. June 2010 this issue. Nebula: Cloud Computing p.1. Better Business with EA p.2 ...

28

Salt modulates the stability and lipid binding affinity of the adipocyte lipid-binding proteins  

Science.gov (United States)

Adipocyte lipid-binding protein (ALBP or aP2) is an intracellular fatty acid-binding protein that is

2003-01-01

29

The Primary Patency and Fracture Rates of Self-Expandable Nitinol Stents Placed in the Popliteal Arteries, Especially in the P2 and P3 Segments, in Korean Patients  

UK PubMed Central (United Kingdom)

ObjectiveWe wanted to evaluate the status of self-expandable nitinol stents implanted in the P2 and P3 segments of the popliteal artery in Korean patients.Materials...Full Text Available

2011-03-01

30

Pharmacological Properties and Physiological Function of a P2X-Like Current in Single Proximal Tubule Cells Isolated from Frog Kidney  

UK PubMed Central (United Kingdom)

Although previous studies have provided evidence for the expression of P2X receptors in renal proximal tubule, only one cell line study has provided functional evidence. The current study investigated...Full Text Available

2010-10-01

31

P2X1 receptor mobility and trafficking; regulation by receptor insertion and activation  

UK PubMed Central (United Kingdom)

P2X1 receptors for ATP contribute to signalling in a variety of cell types and following stimulation undergo rapid desensitisation (within 1 s), and require ∼5 min to recover. In HEK293 cells...Full Text Available

2010-06-01

32

Discrimination between UTP- and P2-purinoceptor-mediated depolarization of rat superior cervical ganglia by 4,4'-diisothiocyanatostilbene-2,2'- disulphonate (DIDS) and uniblue A.  

UK PubMed Central (United Kingdom)

1. Using a grease-gap recording technique we have investigated the effects of some antagonists of P2-purinoceptors on the depolarization of the rat isolated superior cervical ganglion evoked by 100...Full Text Available

1995-06-01

33

Autocrine regulation of T-cell activation by ATP release and P2X7 receptors  

UK PubMed Central (United Kingdom)

T-cell activation requires the influx of extracellular calcium, although mechanistic details regarding such activation are not fully defined. Here, we show that P2X7 receptors play a key...Full Text Available

2009-06-01

34

Structural studies of some phospho-borate glasses using ultrasonic pulse-echo technique, DSC and IR spectroscopy  

International Nuclear Information System (INIS)

Glasses in the system (95-x) [0.25 Na2O-0.75 B2O3]-x P2O5-5 Fe2O3 (0?x?15 mol%), have been prepared by the melt quenching technique. Elastic properties and FT-IR spectroscopic studies have been employed to study the role of P2O5 on the structure of the glass system. Elastic properties Poisson's ratio, micro-hardness and Debye temperature have been investigated using sound wave velocity measurements at 4 MHz (both longitudinal and shear) at room temperature. The results showed that the density and the molar volume increase as both sound velocities and the determined glass transition temperatures decrease with increasing the contents of P2O5. Infrared spectra of the glasses reveal that the borate network consists of diborate units and is affected by the increase in the concentration of P2O5 content as a second network ...

2009-06-01

35

DNA alterations photosensitized by tetracycline and some of its derivatives  

Energy Technology Data Exchange (ETDEWEB)

Bacteriophage M13 mp10 DNA were irradiated with near-UV light in the presence of tetracycline derivatives and primed with synthetic oligonucleotide to be used for DNA synthesis using Escherichia coli DNA polymerase. Chain terminations were observed by denaturing polyacrylamide gel electrophoresis and mapped precisely. All the synthesis stops occurred before or at the level of guanine residues, showing that the photoreaction mediated by tetracycline derivatives led to a preferential alteration of guanine residues. These lesions were demonstrated to be induced in DNA through a pathway involving singlet oxygen. Tetracycline derivatives also photoinduced the breakage of the DNA sugar-phosphate backbone monitored by the conversion of supercoiled phi X174 DNA to a relaxed form. This lesion was shown to be initiated by hydroxyl radicals. The production of this free radical has been confirmed by electron paramagnetic resonance (EPR) spin trapping experiments using ...

1986-06-01

36

Analysis of forward mutations induced by N-methyl-N'-nitro-N-nitrosoguanidine in the bacteriophage P22 mnt repressor gene  

Energy Technology Data Exchange (ETDEWEB)

We describe the isolation and genetic characterization of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced mutations in the phage P22 mnt repressor gene cloned in plasmid pBR322. Mutations in the mnt repressor gene or its operator on this plasmid, pPY98, confer a tetracycline resistance phenotype, whereas the wild-type plasmid confers tetracycline sensitivity. Cells carrying pPY98 were briefly exposed to MNNG to give 20 to 40% survival and a 50- to 100-fold increase in tetracycline-resistant cells. DNA sequence analysis showed that 29 to 30 MNNG-induced mutations were GC-to-AT transitions and one was an AT-to-GC transition. About 80% of the mutations are in three hotspots. This mutation spectrum is consistent with the proposed mechanism of mutagenic action of MNNG, which involves mispairing of an alkylated base, O/sup 6/-methylguanine. The mnt gene may be a useful target for determining mutagenic specificity at the nucleotide level because (i) forward mutations are easily ...

1986-04-01

37

The Primary Patency and Fracture Rates of Self-Expandable Nitinol Stents Placed in the Popliteal Arteries, Especially in the P2 and P3 Segments, in Korean Patients  

Energy Technology Data Exchange (ETDEWEB)

We wanted to evaluate the status of self-expandable nitinol stents implanted in the P2 and P3 segments of the popliteal artery in Korean patients. We retrospectively analyzed 189 consecutive patients who underwent endovascular treatment for steno occlusive lesions in the femoropopliteal artery from July 2003 to March 2009, and 18 patients who underwent stent placement in popliteal arterial P2 and P3 segments were finally enrolled. Lesion patency was evaluated by ultrasound or CT angiography, and stent fracture was assessed by plain X-rays at 1, 3, 6 and 12 months and annually thereafter. At the 1-month follow-up, stent fracture (Type 2) was seen in one limb (up to P3, 1 of 18, 6%) and it was identified in seven limbs at the 3-month follow-up (Type 2, Type 3, Type 4) (n = 1: up to P2: n = 6: P3). At the 6-month follow-up, one more fracture (Type 1) (up to P3) was ...

2011-04-15

38

Genetic markers for lactation persistency in primiparous Australian dairy cows  

British Library Electronic Table of Contents (United Kingdom)

Good performance in extended lactations of dairy cattle may have a beneficial effect on food costs, health, and fertility. Because data for extended lactation performance is scarce, lactation persistency has been suggested as a suitable selection criterion. Persistency phenotypes were calculated in several ways: P1 was yield relative to an approximate peak, P2 was the slope after peak production, and P3 was a measure derived to be phenotypically uncorrelated to yield and calculated as a function of linear regressions on test-day deviations of days in milk. Phenotypes P1, P2, and P3 were calculated for sires as solutions estimated from a random regression model fitted to milk yield. Because total milk yield, calculated as the sum of daily sire solutions, was correlated to P1 and P2 (r=0.30 ...

2010-01-01

39

Sensitivity limits for voltage control of P2Y receptor-evoked Ca2+ mobilization in the rat megakaryocyte  

UK PubMed Central (United Kingdom)

G-protein-coupled receptor signalling has been suggested to be voltage dependent in a number of cell types; however, the limits of sensitivity of this potentially important phenomenon are unknown. Using...Full Text Available

2004-02-15

40

P2Y2 Nucleotide Receptor-Mediated Responses in Brain Cells  

UK PubMed Central (United Kingdom)

Acute inflammation is important for tissue repair; however, chronic inflammation contributes to neurodegeneration in Alzheimer’s disease (AD) and occurs when glial cells undergo prolonged...Full Text Available

2010-06-01

 
 
 
 
41

P2X purinoceptors mediate an endothelium-dependent hyperpolarization in longitudinal smooth muscle of anterior mesenteric artery in young chickens  

UK PubMed Central (United Kingdom)

Background and purpose:The chicken anterior mesenteric artery contains an outer longitudinal smooth muscle layer, whose neural regulation remains to be elucidated. ATP evokes a depolarization...Full Text Available

2009-10-01

42

Congenital woolly hair without P2RY5 mutation  

UK PubMed Central (United Kingdom)

Congenital woolly hair is a disorder with structural defects of the hair shafts. Curled hairs are noticed at birth or soon after birth and often improve with age. Some cases of woolly hairs are associated...Full Text Available

2009-01-01

43

In vitro and in vivo activities of T4 endonuclease V mutants altered in the C-terminal aromatic region  

International Nuclear Information System (INIS)

Genes encoding mutants of the thymine photodimer repair enzyme from bacteriophage T4 (T4 endonuclease V) having an amino acid substitution (T127M, W128A, W128S, Y129A, K130L, Y131A, Y132A) were constructed by use of a previously obtained synthetic gene and expressed in Escherichia coli under the control of the E. coli tryptophan promoter. An in vitro assay of partially fractionated mutant proteins for glycosylase activity was performed with chemically synthesized substrates containing a thymine photodimer. T127M and K130L showed almost the same activity as the wild-type protein. Although W128S, Y131A, and Y132A were slightly active, W128A and Y129A lost activity. The results indicated that the aromatic amino acids around position 130 may be important for the glycosylase activity. Mutant T127M was purified, and the Km value was found to be of the same order as that of the wild type (10(-8) M). In vivo activities for all mutants were characterized with UV-sensitive ...

44

The pp{yields}K{sup +}{sigma}{sup +}n cross-section from missing-mass spectra  

Energy Technology Data Exchange (ETDEWEB)

We utilize existing inclusive data on K{sup +}-meson momentum spectra of the reaction pp{yields}K{sup +}X at T{sub p}=2.3-2.85 GeV to deduce total cross-sections for pp{yields}K{sup +}{sigma}{sup +}n. The method used to extract those cross-sections is explained and discussed in detail. Our result for T{sub p}=2.85 GeV is consistent with the data point from a direct measurement at the same beam energy. The cross-section obtained for T{sub p}=2.3 GeV is with 13.7{+-}2.3 {mu}b considerably smaller than the value found in a recent experiment by the COSY-11 Collaboration at a somewhat lower beam energy, indicating that the pp{yields}K{sup +}{sigma}{sup +}n reaction cross-section could exhibit a rather unusual energy dependence. (orig.)

2007-05-15

45

Excitation cross sections of Cd"+ ions for the upper and lower states of the Cd II 441.6-nm laser line by electron impact  

International Nuclear Information System (INIS)

The excitation cross sections by electron impact from the Cd II ground state to the laser upper state 5s_2 _2D/sub 5/2/ (Beutler state) and the laser lower state 5p _2P/sub 3/2/ (resonance state) have been measured by using a crossed-beam method of electrons and Cd"+ ions and a photon-counting method. The electron-energy region investigated was from the threshold energy (5.8 eV) for the excitation of the 5p _2P/sub 3/2/ state to 20 eV. It has been shown that the excitation cross section for the ionic Beutler state 5s_2 _2D/sub 5/2/ is of the order of 10"-_1_5 cm_2 and has a sharp peak near the threshold energy for the excitation. The excitation cross section for the resonance state 5p _2P/sub 3/2/ has also been of the order of 10"-_1_5 cm_2 and has a relatively broad maximum.

46

Effect of some tripodal bipyrazolic compounds on C38 steel corrosion in hydrochloric acid solution  

British Library Electronic Table of Contents (United Kingdom)

The corrosion inhibition of C38 steel in molar HCl by N,N-bis[2-(3,5-dimethyl-1H-pyrazol-1-yl)ethyl]buthylamine (P1) and 5-{N,N-bis[2-(3,5-dimethyl-1H-pyrazol-1-yl)ethyl] amino} pentanol (P2) has been investigated at 308?K using electrochemical and weight loss measurements. Measurements show that these compounds act as good inhibitors without changing the mechanism of the corrosion process. Moreover, the inhibiting efficiency increases with the increase in concentration of the studied inhibitors. Compound P2 showed better protection properties even at relatively higher temperatures when compared to P1. The associated activation corrosion and free adsorption energies have been determined. P1 and P2 are adsorbed on the C38 steel surface according to a Langmuir isotherm adsorption model.

2010-01-01

47

Cross sections for electron scattering by atomic potassium  

International Nuclear Information System (INIS)

Electron elastic and collisional excitation cross sections from the ground state of potassium are calculated using the noniterative integral-equation method of Henry, Rountree, and Smith [Comput. Phys. Commun. 23, 233 (1981)] in the electron energy range 4#<=#E#<=#200 eV. Configuration-interaction target wave functions that take account of correlation and polarization effects are used to represent the ground state and the six lowest excited states 4p "2P degree, 5s "2S, 3d "2D, 5p "2P degree, 4d "2D, and 6s "2S. Elastic and discrete excitation cross sections are obtained in a seven-state close-coupling (7CC) approximation. The 7CC elastic and excitation cross sections are compared and contrasted. Near threshold the elastic cross section dominates the resonance, 4s "2S#->#4p "2P degree, and the sum of the other remaining excitation cross sections. ...

48

A Robust and Efficient Trust Management Scheme for Peer-to-Peer Networks  

CERN Document Server

Studies on the large scale peer-to-peer (P2P) network like Gnutella have shown the presence of large number of free riders. Moreover, the open and decentralized nature of P2P network is exploited by malicious users who distribute unauthentic or harmful contents. Despite the existence of a number of trust management schemes in the literature for combating against free riding and distribution of malicious files, these mechanisms are not scalable due to their high computational, communication and storage overhead. These schemes also do not consider effect of trust management on quality-of-service (QoS) of the search. This paper presents a trust management scheme for P2P networks that minimizes distribution of spurious files by a novel technique called topology adaptation. It also reduces search time since most of the queries are resolved within the community of ...

2010-01-01

49

Calcium phosphate glass-ceramics for bioactive coating on a #beta#-titanium alloy  

International Nuclear Information System (INIS)

The formation of a porous coating is the decisive feature for the bio-compatibility of silica-free calcium phosphate glass ceramics on alloy surfaces like the #beta#-Ti structured Ti-29Nb-13Ta-4.6Zr used in this work. The ceramic composition is highly important: 50CaO-40P_2O_5-7Na_2O-3TiO_2 glass powder produces a pore-free coating unable to bind hydroxyapatite, whereas 60CaO-30P_2O_5-7Na_2O-3TiO_2 glass incorporates pores from which a crystalline hydroxyapatite phase can grow over the surface from simulated body fluid (see Figure). (Abstract Copyright [2003], Wiley Periodicals, Inc.)

2003-07-01

50

Ab initio relativistic many-body calculation of hyperfine splitting of ^{113}Cd^+  

CERN Document Server

This work presents accurate ab initio determination of the hyperfine splitting for the ground state and few low-lying excited states of 113Cd+; important candidates for the frequency standard in the microwave region, using coupled-cluster theory (CC) in the relativistic framework. The calculated hyperfine splitting are well in agreement with recent experimental results. We have also carried out the lifetimes of the 5p2P1=2 and 5p2P3=2 states, which are in well agreement with recent experimental result (Moehring et al., PRA 73 023413, 2006). The roles of different electron correlation effects in the determination of these quantities are discussed and their contributions are presented in the CC terms.

2007-01-01

51

Selective stimulation of catecholamine release from bovine adrenal chromaffin cells by an ionotropic purinergic receptor sensitive to 2-methylthio ATP  

UK PubMed Central (United Kingdom)

Background2-Methylthioadenosine 5'-triphosphate (2-MeSATP), formerly regarded as a specific P2Y (metabotropic) purinergic receptor agonist, stimulates Ca2+ influx and...Full Text Available

52

Heat capacities and densities of electrolyte mixtures in aqueous solution - application to the determination of apparent molar heat capacities and volumes for potassium triiodide and dioxoneptunium(V) perchlorate  

Energy Technology Data Exchange (ETDEWEB)

Apparent molar heat capacities for KI[sub 3](aq) were determined from flow-microcalorimetric measurements of KI(aq) solutions containing dissolved iodine. From these, the conventional partial molar heat capacity C[sub p,2][sup [infinity

1993-05-01

53

ATP-gated P2X3 receptors constitute a positive autocrine signal for insulin release in the human pancreatic ? cell  

UK PubMed Central (United Kingdom)

Extracellular ATP has been proposed as a paracrine signal in rodent islets, but it is unclear what role ATP plays in human islets. We now show the presence of an ATP signaling pathway that enhances...Full Text Available

2010-04-06

54

Coordination of U"4"+ in the complex U(P_2W_1_7O_6_1)"1"6"-_2 in solid state and in aqueous solution  

International Nuclear Information System (INIS)

The aim of this work is to understand the reasons for the selectivity shown in the complexation by unsaturated heteropolyanions (HPA) of actinides (An) which are oxidized to the number +IV. Different studies have been carried out, both in solution and in solid state, on P_2W_1_7O_6_1"1"0"- and its complexes with Zn"2"+, UO_2"2"+, Ce"4"+, Th"4"+ and U"4"+ to characterize the nature of the complexation site offered to the cation. Among the actinides(IV), the U"4"+ ion has been selected due to its singular spectroscopic and magnetic properties. An initial series of studies, in solution, using NMR"3"1P has thus enabled us to characterize these complexes and to compare them to those formed with the complexing agent PW_1_1O_3_9"8"-. This body of data allows to identify an identical complexation site for both ligands. An analysis of the NMR"3"1P spectrum of U(P_2W_1_7O_6_1)_2"1"6"- has shown a plane of ...

1996-09-01

55

Nuclear factor of activated T cell (NFAT) transcription proteins regulate genes involved in adipocyte metabolism and lipolysis  

Science.gov (United States)

NFAT involvement in adipocyte physiological processes was examined by treatment with CsA and/or GSK3{beta} inhibitors (Li{sup +} or TZDZ-8), which prevent or increase NFAT nuclear translocation, respectively. CsA treatment reduced basal and TNF{alpha}-induced rates of lipolysis by 50%. Adipocytes preincubated with Li{sup +} or TZDZ-8 prior to CsA and/or TNF{alpha}, exhibited enhanced basal rates of lipolysis and complete inhibition of CsA-mediated decreased rates of lipolysis. CsA treatment dramatically reduced the mRNA levels of adipocyte-specific genes (aP2, HSL, PPAR{gamma}, ACS and Adn), compared with control or TNF{alpha}-treatment, whereas Li{sup +} pretreatment blocked the inhibitory effects of CsA, and mRNA levels of aP2, HSL, PPAR{gamma}, and ACS were found at or above control levels. NFAT nuclear localization, assessed by EMSA, confirmed that CsA or Li{sup +} treatments inhibited or increased ...

2007-09-21

56

Large-p heavy-quark production in two-photon collisions  

International Nuclear Information System (INIS)

The next-to-leading-order (NLO) cross section for the production of heavy quarks at large transverse momenta #gamma##gamma# collision is calculated with perturbative fragmentation functions (PFF). This approach allows for a resummation of terms #propor to# #alpha#_sln(p"2/m"2) which arise in NLO from collinear emission of gluons by a heavy quark at large p or from almost collinear branching of photons or gluons into heavy-quark pairs. It is presented single-inclusive distributions in p and rapidity including direct and resolved photons for #gamma##gamma# production of heavy quarks at e"+e"-colliders and at high-energy #gamma##gamma# colliders. The results are compared with fixed- order calculation for m finite including QDC radiative corrections. The two approaches differ in the definitions and relative contributions of the direct and resolved terms, but essentially agree in their sum. The resummation of the ...

2002-05-01

57

From waste minimization to ISO 14000: Taiwan`s experience and prospects  

Energy Technology Data Exchange (ETDEWEB)

Taiwan has completed a very successful five-year industrial waste minimization (IWM) demonstration and promotion project sponsored by the government. From 1990 to 1995, the project successfully disseminated the IWM concept of pollution prevention (P2) to industries. It effectively reduced industrial waste while significantly benefitting the economy by assisting industries in implementing in-plant IWM programs. In July 1995, the second stage of the five-year IWM and ISO 14000 promotion project was initiated for further promoting the IWM, P2, and cleaner production and, in particular, coping with the upcoming international environmental management standards (ISO 14000). To assist industries in establishing an environmental management system (EMS) and accumulating related experience, an EMS pilot demonstration project of five model industries and an ISO 14001 EMS demonstration and promotion project for 22 ...

1996-12-31

58

Electron-impact excitation of multiply-charged ions using energy loss in merged beams: e+Si"3"+(3s"2S_1_/_2)#->#e+Si"3"+(3p"2P_1_/_2_,_3_/_2)  

International Nuclear Information System (INIS)

For the first time absolute total cross sections for electron-impact excitation of a multiply-charged ion have been measured using an electron-energy-loss technique. Measurements were made near threshold for the process e + Si"3"+(3s"2S_1_/_2) #-># e+Si"3"+(3p"2P_1_/_2_,_3_/_2)-8.88 eV. The 10"-"1"5 cm"2 measured cross section agrees with results of 7-state close coupling calculations to better than the #+-#20% (90% CL) total uncertainty of the measurements. Convoluting the theoretical curve with a Gaussian energy distribution indicates an energy width of 0.15 < or approx. #DELTA#E < or approx. 0.20 eV. (orig.).

59

Changes in intracellular Ca2+ levels induced by cytokines and P2 agonists differentially modulate proliferation or commitment with macrophage differentiation in murine hematopoietic cells.  

Science.gov (United States)

The role of intracellular Ca2+ (Ca2+i) on hematopoiesis was investigated in long term bone marrow cultures using cytokines and agonists of P2 receptors. Cytokines interleukin 3 and granulocyte/macrophage colony stimulator factor promoted a modest increase in Ca2+i concentration ([Ca2+]i) with activation of phospholipase Cgamma, MEK1/2, and Ca2+/calmodulin kinase II. Involvement of protein kinase C was restricted to stimulation with interleukin 3. In addition, these cytokines promoted proliferation (20 times) and an increase in the Gr-1(-)Mac-1+ population with participation of gap junctions (GJ). Nevertheless ATP, ADP, and UTP promoted a large increase in [Ca2+]i, moderate proliferation (6 times), a reduction in the primitive Gr-1(-)Mac-1(-)c-Kit+ population, and differentiation into macrophages without participation of GJ. It is likely that Ca2+i participates as a regulator of hematopoietic signaling: moderate increases in [Ca2+]i would be ...

2008-09-05

60

Active packaging of ground beef patties by edible zein films incorporated with partially purified lysozyme and Na2EDTA  

British Library Electronic Table of Contents (United Kingdom)

Summary In this study, antimicrobial activity of zein films incorporated with partially purified lysozyme and disodium ethylenediaminetetraacetic acid (Na2EDTA) has been tested on selected pathogenic bacteria and refrigerated ground beef patties. The developed films containing 700-g-cm-2 lysozyme and 300-g-cm-2 Na2EDTA showed antimicrobial activity on Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella typhimurium. The application of lysozyme and Na2EDTA incorporated zein films on beef patties significantly decreased total viable counts (TVC) and total coliform counts after 5-days of storage compared to those of control patties (P-2EDTA or Na2EDTA alone significantly slowed down the oxidative changes in patties during storage (P-P-2EDTA for active packaging of refrigerated mea...

2011-01-01

 
 
 
 
61

Absolute cross sections for near-threshold electron-impact excitation of the 2s"2S#->#2p"2P transition in C"3"+  

International Nuclear Information System (INIS)

Absolute total cross sections for electron-impact excitation of the 2s"2S#->#2p"2P transition in C"3"+ were measured from 7.35 eV to 8.45 eV using the merged electron-ion-beams energy-loss technique. The results settle the discrepancy between two previous experiments using the crossed-beams fluorescence method, being in very good agreement with the older results [P. O. Taylor, D. Gregory, G. H. Dunn, R. A. Phaneuf, and D. H. Crandall, Phys. Rev. Lett. 39, 1256 (1977)] but less so with the more recent ones [D. W. Savin, L. D. Gardner, D. B. Reisenfeld, A. R. Young, and J. L. Kohl, Phys. Rev. A 51, 2162 (1995)]. The present measurements are also in good agreement with unitarized Coulomb-Born and close-coupling calculations. copyright 1998 The American Physical Society.

62

PBAR: A superconducting magnet spectrometer for cosmic ray antiproton studies  

Energy Technology Data Exchange (ETDEWEB)

We describe the PBAR balloon-borne magnet spectrometer flown on August 13-14, 1987 to measure the abundance of cosmic ray antiprotons in the energy interval 100-1580 MeV at the top of the atmosphere. The limits first reported have been improved to an overall limit of anti p/p<2.0x10{sup -5} (85% CL). We summarize the overall design and performance of the PBAR spectrometer, which had the unique ability to establish the mass of each singly charged cosmic ray, as well as to reject spurious antimatter candidates caused by hard scatterings within the instrument. (orig.).

1990-09-15

63

Interpretation of neutron activation cross-sections with pre-equilibrium consideration  

International Nuclear Information System (INIS)

The aim of the present is to consider the influence of pr equilibrium processes on the activation cross-sections of "2"7 Al(n,p) "2"7 Mg, "5"1 V(n,p) "5"1"m Ti, "8"8 Sr(n,p) "8"8"m Rb, "9"4 Zr(n,p) "9"4"m Y and "9"7"Mo(n,p) "9"7"m Nb in the neutron energy range 13 to 15 MeV. Comparison with recent measurements is given for all considered isotopes. 2 figs., 1 tab.

64

Identification of the #pi#g_9_/_2 band new levels in "1"2"1Cs  

International Nuclear Information System (INIS)

The #pi#g_9_/_2 band structure in "1"2"1Cs is obtained. The experiment was performed at the HI-13 MV tandem accelerator of China Institute of Atomic Energy. The excited states of "1"2"1Cs were populated via the reaction "1"1"2Sn("1"2C, p2n). The #gamma#-#gamma# coincidence data were taken with five BGO-AC HpGe detectors at beam energy of 60 Mev. The energy-level schemes of "1"2"1Cs is presented.

1992-01-01

65

Evolution of ASTEC V1.2 rev.1 code for WWER-1000 reactors/SBO sequence  

International Nuclear Information System (INIS)

In this paper a comparison between calculations of severe accidents occurred from WWER-1000 with ASTEC code specified for an event of full unloading with relief valves stuck opened with no hydroaccumulators intervention is presented. The purpose of the analyses provided is to present the relationship between the improvements of the actual version (ASTEC Vl.2 rev. 1) and ASTEC V1.1 p2 like: code modifications, incoming data improvements. Such discrepancies are to be examined. Case by case suggestions for ASTEC improvements are to be provided.

2006-06-14

66

Crystal structure of telluric acid ammonium phosphate (TAAP) in the paraelectric phase  

Energy Technology Data Exchange (ETDEWEB)

Crystal structure of monoclinic telluric acid ammonium phosphate (TAAP) has been studied by an X-ray diffraction method in the paraelectric phase at 333 K and in the ferroelectric phase at room temperature. It is confirmed that the space group in the paraelectric phase is P2/n. Comparison of the results between the two temperatures suggests that the plane which is made by the PO{sub 4} tetrahedron linked by hydrogen bonds has an important role for the ferroelectric phase transition. This plane is almost parallel to the direction of spontaneous polarization.

2005-01-15

67

Event-related potential correlates of serial-position effects during an elaborative memory test.  

Science.gov (United States)

Twenty undergraduate students participated in an elaborative learning test to evaluate the relationship between electrical brain activity and subsequently recalled and not-recalled words. Data collected from the midline (Fz, Cz, Pz) and lateral scalp sites (F3, F4, C3, C4, P3, P4) were analysed. The difference between event-related potentials (ERPs) elicited by subsequently recalled and not-recalled words, the ERP memory effect, was evaluated for each portion (primacy, plateau and recency) of the serial-position curve (SPC). We compared peak amplitudes for the P1, N1, P2, N400, P3 and frontal positive slow wave (FPSW) components. The electrophysiological data support the hypothesis that different mechanisms underlie primacy and recency effects during free recall paradigms. There was no support for the hypothesis that an association arises between memory and the FPSW when subjects utilise elaborative learning strategies. The ...

2002-10-01

68

Preparation of tetravalent praseodymium in potassium phosphotungstate aqueous solutions  

International Nuclear Information System (INIS)

The possibility has been studied of oxidizing Prsup((3)) to Prsup((4)) when Prsup((3)) is added to an aqueous weakly-acid solution containing K_1_0[P_2W_1_7O_6_1H_2O] x H_2O. A sharp change in the solution colouring has been observed as a result of Prsup((3)) oxidation to Prsup((4)). The absorption spectra of solution of Prsup((4)) have been recorded and the values of the molar extinction coefficients have been determined. On storage of the solution, Prsup((4))reduces to Prsup((3)) with a half-reduction period of 0.2-0.5 hour.

1977-01-01

69

P2Y receptors on astrocytes and microglia mediate opposite effects in astroglial proliferation  

British Library Electronic Table of Contents (United Kingdom)

Nucleotides released upon brain injury signal to astrocytes and microglia playing an important role in astrogliosis, but the participation of microglia in the purinergic modulation of astrogliosis is still unclear. Highly enriched astroglial cultures and co-cultures of astrocytes and microglia were used to investigate the influence of microglia in the modulation of astroglial proliferation mediated by nucleotides. In highly enriched astroglial cultures, adenosine-5?-triphosphate (ATP), adenosine 5?-O-(3-thio)-triphosphate (ATP?S), adenosine 5?-O-(3-thio)-diphosphate (ADP?S; 0.01?1?mM), and adenosine-5?-diphosphate (ADP; 0.1?1?mM) increased proliferation up to 382%, an effect abolished in co-cultures containing 8% of microglia. The loss of ATP proliferative effect in co-cultures is supporte...

2011-01-01

70

Interface-induced conversion of infrared to visible light at semiconductor interfaces  

International Nuclear Information System (INIS)

Efficient, low-temperature conversion of infrared light into visible light (red, orange, green) is reported at single heterojunctions and undoped quantum wells of GaAs and ordered Al_xGa_1_-_xInP_2; an increase in photon energy of 700 meV is obtained. The signal originates from the high-band-gap layers and disappears only if the excitation energy is tuned below the GaAs band gap. The intensity of the up-converted photoluminescence (PL) is found to decrease significantly slower with increasing temperature than that of the regular PL and it remains observable up to 200 K. Interface-induced cold Auger processes along with the presence of trapped states for both electrons and holes in these ordered alloys account for this nonlinear mechanism. A colinear double-beam experiment confirms this. copyright 1996 The American Physical Society.

71

In situ Raman spectroscopy investigation of bioactive glass reactivity: Simulated body fluid solution vs TRIS-buffered solution  

British Library Electronic Table of Contents (United Kingdom)

In the present contribution, the innovative in situ Raman micro-spectroscopy was applied to investigate the in vitro reactivity of various bioactive glasses. All the investigated glasses belonged to the Na2O\\K2O-CaO-P2O5-SiO2 system, but contained sensibly different percentages of network modifiers. The glasses were immersed for increasing times, up to 96h, in simulated body fluid (SBF) and in tris-buffered (TRIS) solution. In this way, two fundamental items were addressed, i.e. the effect of the glass composition and the nature of the soaking fluid on the overall reactivity. As regards the SBF, all the glasses were able to promote the formation of a hydroxyl-carbonate apatite (HCA) surface layer in very short times. The reaction rate was particularly quick for the 45S5 Bioglass and for it...

2011-01-01

72

Genome-wide association study identifies variants in the CFH region associated with host susceptibility to meningococcal disease  

British Library Electronic Table of Contents (United Kingdom)

Meningococcal disease is an infection caused by Neisseria meningitidis. Genetic factors contribute to host susceptibility and progression to disease, but the genes responsible for disease development are largely unknown. We report here a genome-wide association study for host susceptibility to meningococcal disease using 475 individuals with meningococcal disease (cases) and 4,703 population controls from the UK. We performed, in Western European and South European cohorts (consisting of 968 cases and 1,376 controls), two replication studies for the most significant SNPs. A cluster of complement factor SNPs replicated independently in both cohorts, including SNPs within complement factor H (CFH) (rs1065489 (p.936DP = 2.2 ? 10?11) and in CFH-related protein 3 (CFHR3)(rs426736, P = 4.6 ...

2010-01-01

73

Fermion-fermion and boson-boson amplitudes: surprising similarities  

CERN Document Server

Amplitudes for fermion-fermion, boson-boson and fermion-boson interactions are calculated in the second order of perturbation theory in the Lobachevsky space. An essential ingredient of the model is the Weinberg's 2(2j+1)-component formalism for describing a particle of spin j. The boson-boson amplitude is then compared with the two-fermion amplitude obtained long ago by Skachkov on the basis of the Hamiltonian formulation of quantum field theory on the mass hyperboloid, p_0^2 - p^2=M^2, proposed by Kadyshevsky. The parametrization of the amplitudes by means of the momentum transfer in the Lobachevsky space leads to same spin structures in the expressions of T-matrices for the fermion case and the boson case. However, certain differences are found. Possible physical applications are discussed.

2007-01-01

74

Evaluation of cortical current density imaging methods using intracranial electrocorticograms and functional MRI  

British Library Electronic Table of Contents (United Kingdom)

Objective:EEG source imaging provides important information regarding the underlying neural activity from noninvasive electrophysiological measurements. The aim of the present study was to evaluate source reconstruction techniques by means of the intracranial electrocorticograms (ECoGs) and functional MRI.Methods:Five source imaging algorithms, including the minimum norm least square (MNLS), LORETA with Lp-norm (p equal to 1, 1.5 and 2), sLORETA, the minimum Lp-norm (p equal to 1 and 1.5; when p=2, the MNLS method is mathematically equivalent to the minimum Lp-norm) and L1-norm (the linear programming) methods, were evaluated in a group of 10 human subjects, in a paradigm with somatosensory stimulation. Cortical current density (CCD) distributions were estimated from the scalp somatosensor...

2007-01-01

75

Electron-impact excitation of Si"3"+(3s#->#3p) using a merged-beam electron-energy-loss technique  

International Nuclear Information System (INIS)

For the first time, absolute cross sections for electron-impact excitation of a multiply charged ion have been measured using an electron-energy-loss technique. Cross sections for e+Si"3"+(3s "2S_1_/_2)#->#e+Si"3"+(3p "2P_1_/_2_,_3_/_2)-8.88 eV have been measured with an accuracy of #+-#20% (at 90% confidence level) over a narrow energy range (#+-#0.6 eV) about the threshold energy with an energy resolution of 0.2 eV. Results are in good agreement with close-coupling calculations.

76

Calculation of neutron and gamma-ray emission spectra produced by p +2''2'Al reactions  

International Nuclear Information System (INIS)

As a contribution to the US/Japan cooperative program in fusion neutronics, we have prepared a library of multigroup neutron cross sections, scattering matrices, and covariances (uncertainties and their correlations). This 74-group library, called COVFILS-2, is being used at Los Alamos and at the University of California at Los Angeles in the sensitivity and uncertainty analysis of the Li_2O integral experiment recently performed at the Fast Neutron Source (FNS) in Japan. Another intended use of this library is in the estimation of the uncertainty in key performance parameters (such as breeding ratio) of conceptual fusion reactors. The 14 materials included in the first version of COVFILS-2 are H, "6Li, "7Li, Be, C, N, O, Na, Al, Si, Cr, Fe, Ni, and Pb.

1985-01-01

77

Assessment of cost savings of DOE's return-on-investment program  

Energy Technology Data Exchange (ETDEWEB)

The U.S. Department of Energy (DOE) Office of Pollution Prevention (EM-77) created is successful internally competed program to fund innovative projects based on projected returns. This is called the Return-on-Investment (ROI) program. EM-77 conducted a successful ROI pilot, developed and implemented sound management practices, and successfully transferred the program to several Operations Offices. Over the past 4 years sites have completed 262 ROI projects (costing $18.8 million) with claimed first-year savings of $88 million and claimed life cycle savings exceeding $300 million. EM-77 requested that Oak Ridge National Laboratory perform an independent evaluation of the site-led, DOE-HQ-funded pollution prevention (P2) ROI program to assist the Department in determining whether claimed savings are real.

1999-08-01

78

Alpha-induced reactions in iridium  

Energy Technology Data Exchange (ETDEWEB)

The excitation function of ({alpha},{ital xn}) reactions on {sup 191}Ir (abundance 37.3%) and on {sup 193}Ir (abundance 62.7%) has been measured for the 17--55 MeV alpha-particle bombarding energy range. The stacked foil activation technique and {gamma}-ray spectroscopy were used to determine the cross sections. The experimental data were compared with calculated values obtained by means of a geometry-dependent hybrid model. The initial exciton number {ital n}{sub 0}=4 with {ital n}=2, {ital p}=2, and {ital h}=0 gives the best agreements with the presently measured results. To calculate the excitation function theoretically a computer code was used. This set of excitation functions provides a data basis for probing the validity of combined equilibrium and preequilibrium reaction models in a considerable energy range.

1992-05-01

79

A Mediation Framework for Mobile Web Service Provisioning  

CERN Document Server

Web Services and mobile data services are the newest trends in information systems engineering in wired and wireless domains, respectively. Web Services have a broad range of service distributions while mobile phones have large and expanding user base. To address the confluence of Web Services and pervasive mobile devices and communication environments, a basic mobile Web Service provider was developed for smart phones. The performance of this Mobile Host was also analyzed in detail. Further analysis of the Mobile Host to provide proper QoS and to check Mobile Host's feasibility in the P2P networks, identified the necessity of a mediation framework. The paper describes the research conducted with the Mobile Host, identifies the tasks of the mediation framework and then discusses the feasible realization details of such a mobile Web Services mediation framework.

2010-01-01

80

Synthesis, structure, and antitumor activity of novel platinum(II) complexes involving asymmetric chiral diamines as carrier ligands  

Energy Technology Data Exchange (ETDEWEB)

New platinum(II) complexes with asymmetrically substituted chiral diamine ligands A{sub 2}PtX{sub 2}, (A{sub 2}=NH{sub 2}CH(CH{sub 3})CH{sub 2}NH(c-C{sub 5}H{sub 9}) (apcpa), NH{sub 2}CH(CH{sub 3})CH{sub 2}NH(c-C{sub 6}H{sub 11}) (apcha); X{sub 2}=2Cl, isopropylidenmalonate (IPM), 1,1'-cyclobutandicarboxylate (CBDCA) have been synthesized and characterized by means of elemental analyses, infrared and NMR spectroscopies, and X-ray crystallography. The crystal structures of (S-apcha)Pt[CBDCA]{center_dot}3H{sub 2}O (orthorhombic, P2{sub 1}2{sub 1}2(No. 18), a=6.926(3), b=15.243(3), c=19.319(4) A, V=2039.5(10) A{sup 3}, Z=4, R=0.072) and (S-apcha)Pt[IPM]{center_dot}2.5H{sub 2}O (monoclinic, P2/c(No. 13), a=9.882(1), b=18.502(1), c=22.056(1) A, V=4032.8(5) A{sup 3}, Z=8, R=0.093) exhibit that the platinum atoms achieve a typical square planar arrangement with two nitrogen atoms in cis position and ...

1999-12-01

 
 
 
 
81

Photoaffinity labeling of ATP and NAD"+ binding sites on recombinant human interleukin 2  

International Nuclear Information System (INIS)

Interleukin 2 (IL-2) is a T-cell-derived lymphokine critical in the activation and proliferation of T cells, B cells, and lymphokine-activated killer cells. It is a glycoprotein of #approx#15,500 daltons that is synthesized and secreted after activation by antigen or mitogen. By using the analogs 8-azidoadensoine 5'-[#gamma#-"3"2P]triphosphate ([#gamma#-"3"2P]8N_3ATP) and nicotinamide 2-azidoadenine [adenylate-"3"2P]dinucleotide ([#alpha#-"3"2P]2N_3NAD"+) as photoaffinity probes, the authors have detected specific, metal ion-requiring nucleotide binding sites on recombinant human IL-2 (rhIL-2). The specificity of these nucleotide interactions with rhIL-2 was demonstrated by saturation effects and by competition by the parent nucleotides at physiologically relevant concentrations. Saturation of photoinsertion into rhIL-2 occurred at 50 #mu#M [#gamma#-"3"2P]8N_3ATP. Saturation of photoinsertion with ...

82

Enzymatic catalysis in cosolvent modified pressurized organic solvents.  

Science.gov (United States)

An important advantage of carrying out enzymatic catalysis in organic media is the increased solubility of hydrophobic substrates. This study compares a model lipase catalyzed esterification of cholesterol using vinyl acetate (VA) in two such nontraditional media: high-pressure hexane and supercritical (SCF) ethane. The effect of using one of the reactants (VA) as a cosolvent to increase the solubility of the other reactant (cholesterol) in SCF ethane has been investigated. The thermodynamic activity of water (a(w)) in the reaction media was controlled by the direct addition of the salt hydrate pair Na(4)P(2)O(7)/Na(4)P(2)O(7).10H(2)O. The a(w) of the salt hydrate system is shown to be a function of pressure and its variation over the pressure range 104-173 bar has been estimated. The initial reaction rate in pressurized hexane was found to vary linearly with the cholesterol concentration. The reaction ...

1999-11-01

83

The quantum N-body problem with a minimal length  

CERN Document Server

The quantum $N$-body problem is studied in the context of nonrelativistic quantum mechanics with a one-dimensional deformed Heisenberg algebra of the form $[\\hat x,\\hat p]=i(1+\\beta \\hat p^2)$, leading to the existence of a minimal observable length $\\sqrt\\beta$. For a generic pairwise interaction potential, analytical formulas are obtained that allow to estimate the ground-state energy of the $N$-body system by finding the ground-state energy of a corresponding two-body problem. It is first shown that, in the harmonic oscillator case, the $\\beta$-dependent term grows faster with $N$ than the $\\beta$-independent one. Then, it is argued that such a behavior should be observed also with generic potentials and for $D$-dimensional systems. In consequence, quantum $N$-body bound states might be interesting places to look at nontrivial manifestations of a minimal length since, the more particles are present, the more the system deviates from ...

2010-01-01

84

Structure of Ce-doped Bi/sub 2/(MoO/sub 4/)/sub 3/ as determined by neutron profile refinement  

Energy Technology Data Exchange (ETDEWEB)

The structure of Bi/sub 1.8/Ce/sub 0.2/(MoO/sub 4/)/sub 3/ has been refined with powder neutron diffraction data by the Rietveld method. The structure can be derived by severely distorting the scheelite structure (AMO/sub 4/) and is perhaps better written A/sub (2/3)/phi/sub (1/3)/MO/sub 4/, where phi = cation vacancy. Of the two bismuth atom sites, cerium preferentially occupies the more symmetric of the two (Bi(2) in the structure) with some cerium found in the scheelite subcell vacancies also. This site preference is understood by examining the symmetries of the two Bi sites. Crystal data: monoclinic, space group P2/sub 1//c, Z = 4, a = 7.697(2), b = 11.535(3), c = 11.944(3), ..beta.. = 115.19.

1984-05-01

85

Recent neutron scattering work at the University of Lowell  

International Nuclear Information System (INIS)

Neutron elastic and inelastic scattering cross sections of _2_3_8U and _2_3_2Th have been measured at the University of Lowell for states below 1.8 MeV. A time-of-flight (TOF) spectrometer was used. The disc-shaped scatterer was oriented to optimize energy resolutions for 200-to-500-keV neutrons. Neutrons were obtained via the _7Li(p,n)_7Be reaction. Targets were prepared by in-situ evaporation of Li onto a Ta backing. During the evaporation, the target thickness was monitored using a 5-m-flight-path TOF spectrometer; a typical target had a neutron thickness from 8 to 10 keV for Esub(p) = 2.25.MeV. Spectra were analyzed using the unfolding code TINA; standard peak shapes were obtained from codes LAGUE and LAPA. Results obtained will be presented.

1981-11-23

86

Radiolabeling of oligofructans with CO sub 2 in excised wheat leaves  

Energy Technology Data Exchange (ETDEWEB)

The authors studied the flow of carbon ({sup 14}C) from CO{sub 2} through sucrose to oligofructans in wheat (Triticum aestivum L.) leaves that had been induced by excision to accumulate fructan. Leaves were either labeled for 30 min and then chased in ambient air for 2 h or labeled continuously for 2 h. Fructans were chromatographed on BioGel P2 column. During continuous labeling, the specific activity was highest in sucrose and decreased for fructans with DP 3 to 5, and each increased linearly with time for 2 h. During the chase period, sucrose specific activity increased for 60 min and then declined while oligofructans gained in specific activity through the 2 h period. After 30 min of labeling, the specific activity of fructose moiety of sucrose was equal to glucose but, in oligofructans, those of the individual fructose units were not. The results may provide insight into the fructosyl donor-acceptor pool relations in wheat leaves.

1989-04-01

87

Polypropylene/paper laminates as high voltage power cable insulation  

Energy Technology Data Exchange (ETDEWEB)

A study was carried out to identify optimal laminate/oil combinations for low pressure oil filled and high pressure pipe-type cables, and to establish taping criteria under factory conditions. Laminates from two sources were evaluated in conjunction with Sun XX high viscosity mineral oil and Cosden 015SH polybutene oil for high pressure type cables, and Dussek TC-2288N mineral oil, Shell P2 dedecylbenzene and Chevron DF-100 alkylbenzene for low pressure oil-filled cables. Swelling tests, infrared analysis, dissipation and dielectric measurements, impulse tests and a taping trial were performed. It was found that there is no appreciable difference in performance of both laminates when tested on model cables in a variety of oils. Both types exhibited superior aging characteristics vis-a-vis cellulose paper when tested with a variety of oils and at temperatures up to 105{degree}C. For pipe type cable, high viscosity polybutene is the most suitable ...

1983-08-01

88

Phosphorus Immobilization and Soil Aggregation in Chemically Amended Poultry Litter Used in Corn/Soybean Rotation  

British Library Electronic Table of Contents (United Kingdom)

Excessive use of poultry litter (PL) on agricultural land is known to cause eutrophication of surface waters. Consequently, both poultry producers and PL users have to meet strict state and federal guidelines on litter storage and land application. This study examined the environmental benefits of adding lime, alum, ferrous sulfate, fly ash (FA), fluidized bed ash (FBA) and soil fix (SF) to PL for immobilizing excess phosphorus (P) while providing sufficient nutrients for proper growth of soybean [Glycine max (L.)] and corn [Zea mays (L.)] on a rotation. Amending PL with lime, alum, SF, FA and FBA significantly (p>0.05) increased corn and soybean yield. In contrast control plots that received a 10-10-10 (N-P2O5-K2) fertilizer showed lower yield and corn quality. Increased yield was ob...

2011-01-01

89

Measurement and analysis of alpha particle induced reactions on praseodymium  

International Nuclear Information System (INIS)

Excitation functions (EFs) for the reaction "1"4"1Pr(#alpha#, n)"1"4"4Pm and "1"4"1Pr(#alpha#, 2n)"1"4"3Pm have been measured using "1"4"1Pr as a target up to 50 MeV #alpha#-particle energy. Stacked foil activation technique and #gamma#-ray spectroscopy using 100 cm"3 Ge (Li) detector, has been used. Excitation functions are also calculated theoretically using Blann's geometry dependent hybrid (GDH) model code ALICE-91, with and without inclusion of pre-equilibrium particle emission. It has been observed that high-energy tails of the EFs are dominated by pre-equilibrium reaction mechanism. With the accepted set of input parameter, initial exciton number n_0=4 (2p + 2n + 0h), a good qualitative agreement is found. However, quantitative agreement for this magic nucleus"1"4"1Pr is also good when theoretical calculations are normalized by a multiple factor of 0.5. (author)

2005-04-01

90

Lithium ion conductive glass?ceramics with Li3Fe2(PO4)3 and YAG laser-induced local crystallization in lithium iron phosphate glasses  

British Library Electronic Table of Contents (United Kingdom)

The glasses with the composition of 37.5Li2O?(25?x)Fe2O3?xNb2O5?37.5P2O5 (mol%) (x=5,10,15) are prepared, and it is found that the addition of Nb2O5 is effective for the glass formation in the lithium iron phosphate system. The glass?ceramics consisting of Nasicon-type Li3Fe2(PO4)3 crystals with an orthorhombic structure are developed through conventional crystallization in an electric furnace, showing electrical conductivities of 3?10?6?Scm?1 at room temperature and the activation energies of 0.48?eV (x=5) and 0.51?eV (x=10) for Li+ ion conduction in the temperature range of 30?200??C. A continuous wave Nd:YAG laser (wavelength: 1064?nm) with powers of 0.14?0.30?W and a scanning speed of 10??m/s is irradiated onto the surface of the glasses, and the formation of Li3Fe2(PO4)3 crystals is c...

2008-01-01

91

Inhibition of calmodulin - regulated calcium pump activity in rat brain by toxaphene  

International Nuclear Information System (INIS)

In vivo effects of toxaphene on calcium pump activity in rat brain synaptosomes was studied. Male Sprague-Dawley rats were dosed with toxaphene at 0,25,50, and 100 mg/kg/day for 3 days and sacrificed 24 h after last dose. Ca"2"+-ATPase activity and "4"5Ca uptake were determined in brain P_2 fraction. Toxaphene inhibited both Ca"2"+-ATPase activity and "4"5Ca"2"+ uptake and the inhibition was dose dependent. Both substrate and Ca"2"+ activation kinetics of Ca"2"+-ATPase indicated non-competitive type of inhibition as evidenced by decreased catalytic velocity but not enzyme-substrate affinity. The inhibited Ca"2"+-ATPase activity and Ca"2"+ uptake were restored to normal level by exogenously added calmodulin which increased both velocity and affinity. The inhibition of Ca"2"+-ATPase activity and Ca"2"+ uptake and restoration by calmodulin suggests that toxaphene may impair active calcium transport mechanisms by decreasing regulator protein ...

1986-04-13

92

Including the relativistic kinetic energy in a spline-augmented plane-wave band calculation  

International Nuclear Information System (INIS)

The first-order relativistic correction to the kinetic energy of an electron, the mass-velocity term, is not bounded from below. It can, therefore, not be used within a variational framework. To overcome this deficiency we developed a method to include the entire relativistic kinetic energy #sq root#(p"2c"2+m_0"2c"4)-m_0c"2 in a spline-augmented plane-wave band calculation. The first results for silver are quite promising, especially for d and p states: The analysis of the energies of the core states as well as of the valence band structure suggests that the energies of d bands are reproduced within 1 mRy. However, the combination of the relativistic kinetic energy with the Darwin term leads to energies which are too low for s-like valence states by 10 mRy. Therefore, the s and d valence band complex is spread out and the Fermi level is lowered by the same amount as the s states. We expect to overcome these deficiencies in future investigations ...

93

Excitation cross sections for the ns "2S#->#np "2P resonance transitions in Mg"+ (n=3) and Zn"+ (n=4) using electron-energy-loss and merged-beams methods  

International Nuclear Information System (INIS)

Electron-excitation cross sections are reported for the 3s "2S#->#3p "2P(h,k) resonance transition in Mg"+ at energies from threshold (4.43 eV) to approximately 9 times threshold (40.0 eV). The electron-energy-loss merged-beams technique used in these measurements is described in detail. In addition, the method of separating contributions of the elastically scattered (Coulomb) and the inelastically scattered electrons in the present Mg"+ case and previously reported Zn"+ results [Phys. Rev. Lett. 67, 30 (1991)] is described. Comparisons in the experimental energy range are made for Mg"+ with the two five-state close-coupling theoretical calculations carried out herein, and with other published close-coupling, distorted-wave, and semiempirical calculations. The present Mg"+ cross sections and Zn"+ cross sections from earlier measurements are tabulated.

94

Equilibrium and pre-equilibrium emissions in proton-induced reactions on "2"0"3","2"0"5Tl  

International Nuclear Information System (INIS)

In this study, the excitation functions for the reactions "2"0"3Tl(p, n)"2"0"3Pb, "2"0"5Tl(p, 3n)"2"0"3Pb, "2"0"3Tl(p, 2n)"2"0"2Pb, "2"0"5Tl(p, 4n)"2"0"2Pb, "2"0"3Tl(p, 3n)"2"0"1Pb, "2"0"5Tl(p, 5n)"2"0"1Pb, "2"0"3Tl(p, 4n)"2"0"0Pb and "2"0"5Tl(p, 6n)"2"0"0Pb have been calculated using pre-equilibrium and equilibrium reactions mechanisms. Calculated results based on hybrid model, geometry-dependent hybrid model and cascade-exciton model have been compared with the experimental data. (author)

2009-02-01

95

Entropy, confinement, and chiral symmetry breaking  

CERN Document Server

This paper studies the way in which confinement leads to chiral symmetry breaking (CSB) through a gap equation. We argue that entropic effects cut off infrared singularities in the standard confining effective propagator $1/p^4$, which should be replaced by $1/(p^2+m^2)^2$ for a finite mass $m\\sim K_F/M(0)$ [$M(0)$ is the zero-momentum value of the running quark mass]. Extension of an old calculation of the author yields a specific estimate for $m$. This cutoff propagator shows semi-quantitatively two critical properties of confinement: 1) a negative contribution to the confining potential coming from entropic forces; 2) an infrared cutoff required by gauge invariance and CSB itself. Entropic effects lead to a proliferation of pion branches and a $\\bar{q}q$ condensate, and contribute a negative term $\\sim -K_F/M(0)$ to the effective pion Hamiltonian allowing for a massless pion in the presence of positive kinetic energy and string energy. ...

2010-01-01

96

Design for environment for the National Ignition Facility  

Energy Technology Data Exchange (ETDEWEB)

The National Ignition Facility (NIF) will be a U.S. Department of Energy (DOE) national center for inertial confinement fusion (ICF) and other research into the physics of high temperatures and high densities, and a vital element of the DOE`s nuclear weapons Stockpile Stewardship and Management Program. It will be used by scientists from a numerous different institutions and disciplines to support research advancements in national security, energy, basic science, and economic development. Multiple powerful laser beams will `ignite` small fusion targets, helping liberate more energy than is required to initiate the fusion reactions. This paper discusses the Design for Environment process for NIF, some of the subsequent activities resulting from the initial study, and a few of the lessons learned from this process. Subsequent activities include the development of a Pollution Prevention and Waste Minimization Plan (P2/WMin) for the facility, which ...

1998-05-01

97

Design characteristics of the ion cyclotron system for the KSTAR Tokamak  

Energy Technology Data Exchange (ETDEWEB)

The design of the KSTAR (Korea Superconducting Tokamak Advanced Research) tokamak (R{sub 0} = 1.8 m, a= 0.5 m, {kappa} = 2, {delta} = 1.8 B{sub T} = 3.5 T, I{sub p} 2 MA, {tau}{sub pulse} = 300 s) is being undertaken to do long-pulse. high {beta}, advanced tokamak operating-mode fusion physics experiments. The ion cyclotron (IC) system will deliver 6 MW of rf power to the plasma in the 25 - 60 MHz frequency range, using a single four-strap antenna mounted in a midplane port. It will be used for ion heating, fast-wave current drive (FWCD), and mode conversion current drive (MCCD). The phasing between current straps in the antenna will be adjustable quickly during operation to provide the capability of changing the current-drive efficiency. The IC system will be capable of 300 s operation with 12 MW (upgrade) of rf power to the plasma. (author)

1998-07-01

98

Two and 8-azido photoaffinity probes. 1. Enzymatic synthesis, characterization, and biological properties of 2- and 8-azido photoprobes of 2-5A and photolabeling of 2-5A binding proteins  

International Nuclear Information System (INIS)

The 2- and 8-azido trimer 5'-triphosphate photoprobes of 2-5A have been enzymatically synthesized from [#gamma#-"3"2P]2-azidoATP and [#alpha#-"3"2P]8-azidoAPT by 2-5A synthetase from rabbit reticulocyte lysates. Identification and structural determination of the 2- and 8-azido adenylate trimer 5'-triphosphates were accomplished by enzymatic hydrolyses with T2 RNase, snake venom phosphodiesterase, and bacterial alkaline phosphatase. Hydrolysis products were identified by HPLC and PEI-cellulose TLC analyses. The 8-azido photoprobe of 2-5A displaces p_3A_4["3"2P]pCp from RNase L with affinity equivalent to p_3A_3. The 8-azido photoprobe also activates RNase L to hydrolyze poly(U)["3"2P]pCp 50% at 7 /times/ 10/sup /minus/9/ M in core-cellulose assays. The 2- and 8-azido photoprobes and authentic p_3A_3 activate RNase L to cleave 28S and 18S rRNA to specific cleavage products at 10/sup /minus/9/ M in rRNA cleavage assays. The nucleotide binding ...

99

Synthesis, characterization, and crystal structure of neutral rhenium(V) complexes with S-substituted N{sub 2}S{sub 2} ligands  

Energy Technology Data Exchange (ETDEWEB)

Rhenium is technetium`s third row congener and exhibits many of the chemical properties that technetium displays. Theoretically, a Re-PhAT complex will be isostructural with the {sup 99m}Tc PhAT complexes that have been prepared for use as brain imaging agents. A series of neutral rhenium(V) oxo complexes was synthesized by the reaction of ReOBr{sub 4}{sup {minus}} with diamino-thiol-thioether ligands of the type (RSC(CH{sub 3}){sub 2})CH{sub 2}NH(o-C{sub 6}H{sub 4})NHCH{sub 2}C(CH{sub 3}){sub 2}SH. The complexes were characterized by IR, UV/visible, and {sup 1}H and {sup 13}C NMR spectroscopy and by fast-atom-bombardment mass spectroscopy. The single-crystal X-ray structure determination on two of the complexes, where R = CH{sub 2}CH{double_bond}CH{sub 2} and CH{sub 2}CH{sub 2}-CH{sub 3}, showed them to consist of a square pyramidal Re{sup V}ON{sub 2}S{sub 2} core. ReO[CH{sub 2}{double_bond}CHCH{sub 2}SC(CH{sub 3}){sub 2}CH{sub 2}N(o-C{sub 6}H{sub 4})NCH{sub 2}C(CH{sub 3}){sub 2}S], ...

1994-11-23

100

Two and 8-azido photoaffinity probes. 2. Studies on the binding process of 2-5A synthetase by photosensitive ATP analogues  

International Nuclear Information System (INIS)

The photoaffinity probes [#gamma#-"3"2P]2-azidoATP (2-N_3ATP) and [#alpha#-"3"2P]8-azido-ATP (8-N_3ATP) were used to investigate the binding of ATP to highly purified 2-5A synthetase. 2-N_3APT and 8-N_3ATP are substrates for 2-5A synthetase. In this study the authors show that 2- and 8-N_3ATP are competitive inhibitors of the enzymatic conversion of ATP to 2-5A. Ultraviolet irradiation results in the photoinsertion of 2-N_3ATP and 8-N_3ATP into the enzyme. The covalent photoinsertion of [#alpha#-"3"2P]8-N_3ATP into the 2-5A synthetase is proportional to the inactivation of the enzyme as UV irradiation is increased. Photolabeling of 2-5A synthetase is saturated at 1.5 mM 2-N_3ATP and 2.0 mM 8-N_3ATP. Computer analysis of the curvilinear Scatchard plots of the 2-5A synthetase suggest the presence of high-affinity and low-affinity binding sites that may correspond to the acceptor and the 2'-adenylation sites of the enzyme. The competition of ...

 
 
 
 
101

Regulation of NMDA and AMPA receptors during the maturation phase of chicken brain development  

International Nuclear Information System (INIS)

Full text: The maturation of chicken forebrain is protracted and occurs well after synapse formation providing a good model for studying mechanisms of brain maturation. Using microslices from immature (10 day) and adult chicken forebrain prepared after decapitation, we have examined functional properties of NMDA and AMPA receptors by measuring agonist-induced uptake of "4"5Ca"2"+ . The rate and extent of NMDA induced "4"5Ca"2"+ accumulation decreased during maturation with no change in EC_5_0. The rate and extent of the AMPA induced response also decreased with a 60-fold increase in EC_5_0. However, the total NMDA receptor content did not change as indicated by 3 H-MK801 binding and NR1 immunoreactivity in P2 fractions. Similarly, there was no change in the B_m_a_x of "3H-AMPA, though there was a two-fold increase in K_D, and little or no change in the immunoreactivity in GluR1, 2, 2/3 or 4. These results suggest that it is the regulation of ...

2002-02-04

102

Patterns of ovarian morphology in polycystic ovary syndrome: a study utilising magnetic resonance imaging  

Energy Technology Data Exchange (ETDEWEB)

To evaluate and compare MRI-based ovarian morphology in groups of women with polycystic ovary syndrome (PCOS) and controls. All PCOS cases (n = 44) had oligo-amenorrhoea and hyperandrogenism irrespective of ovarian morphology, and fulfilled NIH/Rotterdam diagnostic criteria for PCOS. All control women (n = 40) had normal menses and normoandrogenaemia. All subjects were of white British/Irish origin and pre-menopausal. Group comparisons were based on independent-sample t tests. Polycystic ovarian morphology was defined by at least 12 follicles 2-9 mm in diameter and/or an ovarian volume greater than 10 cm{sup 3}. Ovarian morphology differed significantly in PCOS cases and controls (follicle number geometric mean [SD range] 18.6 [9.9, 35.0] vs 6.6 [3.1, 14.2], unadjusted P = 1.3 x 10{sup -16}; calculated ovarian volume 8.8 cm{sup 3} [5.0, 15.5] vs 5.1 cm{sup 3} [2.5, 10.3], unadjusted P = 3.0 x 10{sup -7}; peripheral follicle location in 55% vs 18% of ovaries, P = 7.9 x 10{sup -6}; ...

2010-05-15

103

Paramagnetic susceptibility simulations from crystal field effects on Nd{sup 3+} in magnesium borate MgNd(BO{sub 2}){sub 5}  

Energy Technology Data Exchange (ETDEWEB)

From the crystal field analysis of optical absorption spectra on monoclinic S.G. P2{sub 1}/c MgNd(BO{sub 2}){sub 5}, where Nd{sup 3+} occupies a single crystallographic position with no symmetry elements, energy level schemes and an expression of the associated wavefunctions for the 4f{sup 3} configuration of Nd{sup 3+} have been derived, considering approximate C{sub 2v} and C{sub s} (C{sub 2}) symmetries. Despite of the low symmetry of Nd{sup 3+}, resulting rms deviations between calculated and experimental levels are very satisfactory. The composition of the crystal field wavefunctions from both sets of phenomenological free-ion and crystal field parameters has been checked through a calculation of the thermal evolution of the paramagnetic susceptibility {chi}, according to the Van Vleck formula. The same simulation was performed with crystal field parameters (CFPs) resulting from the ab initio simple overlap model (SOM), considering the ...

1999-01-01

104

Metastability and dynamics of the shock-induced phase transition in iron  

Energy Technology Data Exchange (ETDEWEB)

The shock-induced {alpha}(bcc){r_arrow}{var_epsilon}(hcp) transition in iron begins at 13 GPa on the Hugoniot. In the two-phase region above 13 GPa, the Hugoniot lies well above the equilibrium surface defined by G{sub {alpha}}=G{sub {var_epsilon}}, with G the Gibbs free energy. Also, the phase transition relaxation time {tau} is uncertain, with estimates ranging from {lt}50 ns to {approx}180 ns. Here we present an extensive study of these important aspects, metastability and dynamics, of the {alpha}-{var_epsilon} transition in iron. Our primary theoretical tools are (a) accurate theoretically based free energies for {alpha} and {var_epsilon} phases of iron and (b) accurate calculations of the wave evolution following planar impacts. We define metastable surfaces for forward and reverse transitions by the condition that the thermodynamic driving force G{sub {alpha}}{minus}G{sub {var_epsilon}} is just balanced by an opposing force resulting from elastic stresses, and we calibrate the ...

1997-02-01

105

Luminescence and laser transition studies of Dy3+:K-Mg-Al fluorophosphate glasses  

International Nuclear Information System (INIS)

Dysprosium ion doped fluorophosphate glasses with compositions of PKMAFDy: (56-x/2) P2O5+17K2O+8Al2O3+(15-x/2)MgO+4AlF3+xDy2O3 (x=0.01, 0.05, 0.1, 1.0 and 2.0 mol%) have been prepared by melt quenching technique and are characterized by optical absorption, emission spectra and fluorescence lifetime measurements. The observed bands in the absorption spectrum are analysed by using free-ion Hamiltonian (HFI) model. The Judd-Ofelt (JO) analysis has been performed and the intensity parameters (??, ?=2, 4, 6) have been evaluated that are used to predict radiative properties. From emission spectra, the effective bandwidth (??eff) and the stimulated emission cross-section (?(?p)) were evaluated. The fluorescence decay from the 4F9/2 level of Dy3+ ions have been measured by monitoring the intense 4F9/2#->#6H13/2 transition (573 nm). The lifetimes (?) are found to decrease with increasing concentration due to concentration quenching. The decay curves ...

2009-02-28

106

Induction of lauric acid omega-hydroxylation by peroxisomal proliferators in bluegill and catfish  

Energy Technology Data Exchange (ETDEWEB)

Peroxisome proliferating agents (PPAs) are a structurally diverse group of chemicals that include environmental chemical contaminants such as certain chlorinated herbicides, solvents and plasticizers. PPAs have previously been shown to induce anti-trout laruci acid hydroxylase immunoreactive proteins in bluegill and catfish. In this investigation, induction of lauric acid hydroxylase activity and immunoreactive proteins was confirmed, and the mass spectral analysis of specific hydroxylation products was performed in order to identify possible species-specific differences in fatty acid metabolism. Male bluegill (Lepomis macrochirus) and channel catfish (Ictalurus punctatus) were administered clofibrate or ciprofibrate 48 hr prior to hepatic or trunk kidney (catfish only) microsome preparation. While no significant differences were observed in male catfish, male bluegill had significant decreases in hematocrit and plasma protein indicating hemodilution due to possible gill or kidney ...

1995-12-31

107

Hanford Site pollution prevention progress report  

Energy Technology Data Exchange (ETDEWEB)

The Richland Operations Office (RL) and Office of River Protection (ORP) are pleased to issue the attached Pollution Prevention Progress Report. We have just met the most aggressive waste reduction and A recycling goals to date and are publishing this report to recognize A the site's progress, and to ensure it will sustain success beyond 1 Fiscal Year 2000. This report was designed to inform the been made by RL and ORP in Waste Minimization (WMin) and Pollution Prevention (P2). RL, ORP and their contractors are committed to protecting the environment, and we reiterate pollution prevention should continue to be at the forefront of the environmental cleanup and research efforts. As you read the attached report, we believe you will see a clear demonstration of RL and ORP's outstanding performance as it has been responsible and accountable to the nation, its employees, and the community in which we live and work. commitment that ...

1999-10-05

108

Growth and characterization of new semi-organic L-proline strontium chloride monohydrate single crystals  

International Nuclear Information System (INIS)

The present communication deals with the synthesis, single crystal growth and characterization of a new nonlinear optical material L-proline strontium chloride monohydrate (L-PSCM). Single crystals have been grown using the slow solvent evaporation technique. Single crystal XRD analysis confirmed that the crystal belongs to the orthorhombic structure with lattice parameter a=6.6966(3) A, b=12.4530(5) A, c=15.2432(5) A and space group P2_12_12_1. Presence of various functional groups in L-PSCM and protonation of the ions were confirmed by Fourier transform infrared spectroscopy (FT-IR) analysis. The melting point of the single crystal was found to be 126 "oC using DSC. Ultraviolet-visible spectral analyses showed that the crystal has low UV cut-off at 226 nm combined with very good transparency of 90% in a wide range. The optical band gap was estimated to be 5.82 eV. Capacitance and dielectric-loss measurements were carried out at different ...

2011-01-01

109

Gas chromatographic detection of organometallic compounds by reactive-flow photometry  

International Nuclear Information System (INIS)

The response of the reactive flow detector (RFD) toward organometallic compounds of several transition metals has been explored, and several of its strong elemental responses have been characterized in detail. The RFDs minimum detectable flow (measured in picograms of metal per second at S/N_p_-_t_-_p=2) is 0.1 for ruthenium, 3 for chromium, 10 for manganese, 5 for nickel, 15 for iron, and 2 for osmium. Typically, the linearity of response spans four orders of magnitude, with atomic selectivity of metal versus carbon ranging from 2 to 3 orders. Response quenching by co-eluting hydrocarbons is not observed. As a demonstration experiment, the common analysis of methylcyclopentadienyl manganese tricarbonyl (MMT) in gasoline is carried out on an RFD system modified for dual-channel operation. The results show that dual-channel operation of the RFD can increase the native elemental selectivity of manganese over carbon by a factor of 100, in ...

2002-02-03

110

Contribution of pulmonary emphysema to functional impairment in patients with severe chronic obstructive pulmonary disease. A high-resolution CT study  

International Nuclear Information System (INIS)

The purpose of the paper is to investigate whether high-resolution CT (HRCT) can detect the subjects with massive emphysematous destruction in a group of patients with severe chronic obstructive pulmonary disease (COPD) and therefor be of help in selecting the candidates to surgical lung volume reduction. Have been examined 40 former smokers with severe COPD (FEV_1#<=#40% of the predicted value, with no major improvement after inhalation of bronchodilators). The emphysema extent score was significantly correlated with the hematocrit value and Tiffeneau index (p<2) in all the 40 patients. The severity of bronchial obstruction was the same in bronchitis and dyspnoeic patients. The subject with chronic bronchitis had milder emphysema (mean extent 35% versus 47% in the dyspnoeic subjects) and a higher frequency of bronchial involvement. A decrease in FEV_1 was significantly correlated with emphysema extent (p<1) in dyspnoeic, but not in ...

1999-01-01

111

Carbomolybdates(III): On Pr{sub 2}[Mo{sub 2}C{sub 3}] and structural relations to Er{sub 2}Mo{sub 2}C{sub 3}; Carbomolybdate(III): Zur Kenntnis von Pr{sub 2}[Mo{sub 2}C{sub 3}] sowie Strukturbeziehungen zu Er{sub 2}Mo{sub 2}C{sub 3}  

Energy Technology Data Exchange (ETDEWEB)

Pr{sub 2}[Mo{sub 2}C{sub 3}] was obtained by arc melting of the elements followed by annealing at 1500 C in arc welded and sealed Mo-ampoules. The compound was characterised by chemical analyses, X-ray diffraction and metallography. The crystal structure (monoclinic, P2{sub 1}/c, Z = 4, a = 597.99(10) pm, b = 665.15(11) pm, c = 1185.60(19) pm and {beta} = 111.631(5) ) represents a new structure type containing a three dimensional polyanion {sub {infinity}}{sup 3}[(Mo{sub 2}C{sub 3}){sup 6-}] with Molybdenum(III) in a distorted tetrahedral coordination by carbo-ligands. Tetrameric building-blocks, Mo{sub 4}C{sub 10}, of edge sharing MoC{sub 4}-tetrahedra are arranged to form layers running parallel to (100). The layers are interconnected along [100] by sharing common apices of the MoC{sub 4} tetrahedra. The coordination polyhedra around praseodymium represent distorted (PrC{sub 5})-arrangements (mono capped tetrahedra and square pyramids). The ...

2004-11-01

112

Synthesis, crystal structures and spectroscopic properties of RbBaTaS{sub 4} and K{sub 2}BaTa{sub 2}S{sub 11}  

Energy Technology Data Exchange (ETDEWEB)

Single crystals of RbBaTaS{sub 4} (1) and K{sub 2}BaTa{sub 2}S{sub 11} (2) were obtained from the reactions of Ta, with in situ formed fluxes of A{sub 2}S{sub 3} (A = K, Rb), BaS, and S at 500 C. Compound 1 crystallizes in the orthorhombic space group Pnma with a = 9.3286(5), b = 7.0391(4), c = 12.4365(7) A, V = 816.6(1) A{sup 3}, Z = 4. Compound 2 crystallizes in the monoclinic space group P2{sub 1}/c with a = 14.5280(10), b = 12.6347(7), c = 17.5148(12) A, {beta} = 94.744(8) , V = 3203.9(4) A{sup 3}, Z = 4. The structure of RbBaTaS{sub 4} (1) consists of isolated tetrahedral [TaS{sub 4}]{sup 3-} anions and Rb{sup +} and Ba{sup 2+} cations. The Ba{sup 2+} cations are surrounded by nine sulfur atoms forming distorted tricapped trigonal prisms, whereas the Rb{sup +} cations are in an irregular environment of ten sulfur atoms. The structure of K{sub 2}BaTa{sub 2}S{sub 11} (2) consists of two different dinuclear [Ta{sub 2}S{sub 11}] units which ...

2010-10-15

113

Microstructure and corrosion resistance of Ni-based alloy laser coatings with nanosize CeO{sub 2} addition  

Energy Technology Data Exchange (ETDEWEB)

Micron-size Ni-base alloy (NBA) powders were mixed with both 1.5 wt.% (hereinafter %) micron-size CeO{sub 2} (m-CeO{sub 2}) and also 1.5% and 3.0% nano-size CeO{sub 2} (n- CeO{sub 2}) powders. These mixtures were coated on low-carbon steel (Q235) by 2.0 kW CO{sub 2} laser cladding. The effects on the microstructures, phases and electrochemical corrosion of the coatings upon the addition of m- and n- CeO{sub 2} powders to NBA (m- and n- CeO{sub 2} /NBA) have been investigated. The results showed that a smooth coating was prepared under suitable processing parameters (P= 2.0 kW, V= 180 mm min{sup -1}) by adding 1.5% n- CeO{sub 2}. In addition to the primary phases of {gamma}-Ni, Cr{sub 23} C{sub 6} and Ni{sub 3} B in the Ni-base alloy coating, CeNi{sub 3} was formed in Ni-base alloy coatings with both n- CeO{sub 2} and m-CeO{sub 2} particles, and CeNi{sub 5} appeared in the coating upon decreasing the size of CeO{sub 2} particles. Well-developed ...

2008-07-01

114

Measurement and analysis of neutron production cross sections and determination of some reaction rates for nuclear astrophysical calculations  

International Nuclear Information System (INIS)

The absolute cross sections of "2"3Na(p,n)"2"3Mg, "2"7Al(p,n)"2"7Si and "3"0Si(#alpha#,n)"3"3S reactions were measured in the incident energy range of 5.05 to 5.80, 5.80 to 6.25 and 3.975 to 6.235 MeV respectively using a spherically shaped 4#pi# neutron detector. In the energy range 5.80 to 7.80 and 6.235 to 11.30 MeV the absolute cross sections of "2"3Na(p,n)"2"3Mg and "3"0Si-(#alpha#,n)"3"3S reactions were determined by optical model calculations. The cross sections of the inverse reactions "2"3Mg(n,p)"2"3Na and "3"3S(n,#alpha#)"3"0Si were also calculated by the same method for the neutron energy range of 10 keV to 7.50 MeV for each reaction. The cross section of the latter reaction in the neutron energy range of 10 keV to 840 keV was also determined from its inverse reaction "3"0Si(#alpha#,n)"3"3S by the application of the detailed balance theorem. The reactions for which the cross sections were determined are of importance in stellar ...

115

2007 SB14 Source Reduction Plan/Report  

Energy Technology Data Exchange (ETDEWEB)

Aqueous solutions (mixed waste) generated from various LLNL operations, such as debris washing, sample preparation and analysis, and equipment maintenance and cleanout, were combined for storage in the B695 tank farm. Prior to combination the individual waste streams had different codes depending on the particular generating process and waste characteristics. The largest streams were CWC 132, 791, 134, 792. Several smaller waste streams were also included. This combined waste stream was treated at LLNL's waste treatment facility using a vacuum filtration and cool vapor evaporation process in preparation for discharge to sanitary sewer. Prior to discharge, the treated waste stream was sampled and the results were reviewed by LLNL's water monitoring specialists. The treated solution was discharged following confirmation that it met the discharge criteria. A major source, accounting for 50% for this waste stream, is metal machining, cutting and grinding operations in the ...

2007-07-24

116

National Ignition Facility Incorporates P2/E2 in Aqueous Parts Cleaning of Optics Hardware  

Energy Technology Data Exchange (ETDEWEB)

When completed, Lawrence Livermore National Laboratory's (LLNL) National Ignition Facility (NIF) will be the world's largest laser with experimental capabilities applicable to stockpile stewardship, energy research, science and astrophysics. As construction of the conventional facilities nears completion, operations supporting the installation of specialized laser equipment have come online. Playing a critical role in the precision cleaning of mechanical parts from the NIF beamline are three pieces of aqueous cleaning equipment. Housed in the Optics Assembly Building (OAB), adjacent to NIF's laser bay, are the large mechanical parts gross cleaner (LMPGC), the large mechanical parts precision cleaner (LMPPC), and the small mechanical parts gross and precision cleaner (SMPGPC). These aqueous units, designed and built by Sonic Systems, Inc., of Newtown, Pennsylvania, not only accommodate parts that vary greatly in size, weight, geometry, surface finish and ...

2001-07-27