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Sample records for aquaculture system electronic

  1. Prospective Analysis of the Aquaculture Sector in the EU. PART 2: Characterisation of Emerging Aquaculture Systems

    STURROCK Helen; Newton, Richard; PAFFRATH Susan; Bostock, John; MUIR James; Young, James; IMMINK Anton; DICKSON Malcolm

    2008-01-01

    This report is based on the outcome of the study on "Prospective analysis of the aquaculture sector in the EU", launched and coordinated by the JRC (IPTS) and carried out by the University of Stirling. The report consists of two parts: 1) "Prospective analysis of the aquaculture sector in the EU ¿ Part 1: Synthesis report", and 2) "Prospective analysis of the aquaculture sector in the EU ¿ Part 2: Characterisation of emerging aquaculture systems." This second report is concerned wit...

  2. THE BIOECONOMICS OF RECIRCULATING AQUACULTURE SYSTEMS

    Richard F. KAZMIERCZAK, Jr.; Caffey, Rex H.

    1996-01-01

    The goal of this study was to produce a detailed aquaculture production model incorporating constraints unique to closed system culture and to conduct a formal economic analysis of closed system operation. Results generated by this model indicate that less than perfect management ability can eliminate the normal advantages associated with using high protein feed. While higher protein levels produce faster growth, and for this reason are often used in the industry, the increased direct feed co...

  3. Optimization of aquaculture systems in Spain

    Izquierdo, M. [Instituto Ciencias de la Construccion Eduardo Torroja, Madrid (Spain); Carrillo, M. [Instituto de Acuicultura de Torre la Sal, Castellon (Spain)

    1997-12-31

    An analysis of present heat production systems, using fossil fuel combustion, employed for sea water heating in Spanish hatcheries is given in this paper and compared to a technical solution based on the employment of a heat pump. Price per unit of produced energy is calculated for each system using liquid and gaseous fuels, and then these prices are compared to the price obtained for a heat pump. The heat pump system is also compared, from the point of view of its precision in maintaining temperatures, to the systems used at the present. A project prototype for thermal conditioning and temperature control in aquaculture rearing tanks is described. (author)

  4. Modeling of TAN in recirculating aquaculture systems by AQUASIM

    Arvin, Erik; Pedersen, Lars-Flemming

    2011-01-01

    Modeling of total ammonium nitrogen (TAN) in recirculating aquaculture systems (RAS) contribute to identifying and quantifying the most important processes and their relative contribution to removal of TAN. AQUASIM is a flexible modular simulation system for water quality in natural and technical...... systems developed by EAWAG (Reichert, 1994). AQUASIM allows simulating complex biological, chemical and physical processes in standardized hydraulic systems. We used AQUASIM to model the steady state TAN concentrations in 12 experimental recirculating aquaculture systems (RAS) operated by DTU AQUA in...

  5. Does aquaculture add resilience to the global food system?

    Troell, Max; Naylor, Rosamond L; Metian, Marc; Beveridge, Malcolm; Tyedmers, Peter H; Folke, Carl; Arrow, Kenneth J; Barrett, Scott; Crépin, Anne-Sophie; Ehrlich, Paul R; Gren, Asa; Kautsky, Nils; Levin, Simon A; Nyborg, Karine; Österblom, Henrik; Polasky, Stephen; Scheffer, Marten; Walker, Brian H; Xepapadeas, Tasos; de Zeeuw, Aart

    2014-09-16

    Aquaculture is the fastest growing food sector and continues to expand alongside terrestrial crop and livestock production. Using portfolio theory as a conceptual framework, we explore how current interconnections between the aquaculture, crop, livestock, and fisheries sectors act as an impediment to, or an opportunity for, enhanced resilience in the global food system given increased resource scarcity and climate change. Aquaculture can potentially enhance resilience through improved resource use efficiencies and increased diversification of farmed species, locales of production, and feeding strategies. However, aquaculture's reliance on terrestrial crops and wild fish for feeds, its dependence on freshwater and land for culture sites, and its broad array of environmental impacts diminishes its ability to add resilience. Feeds for livestock and farmed fish that are fed rely largely on the same crops, although the fraction destined for aquaculture is presently small (∼4%). As demand for high-value fed aquaculture products grows, competition for these crops will also rise, as will the demand for wild fish as feed inputs. Many of these crops and forage fish are also consumed directly by humans and provide essential nutrition for low-income households. Their rising use in aquafeeds has the potential to increase price levels and volatility, worsening food insecurity among the most vulnerable populations. Although the diversification of global food production systems that includes aquaculture offers promise for enhanced resilience, such promise will not be realized if government policies fail to provide adequate incentives for resource efficiency, equity, and environmental protection. PMID:25136111

  6. In-tank aeration, a necessary compliment of loaded systems in an airlift recirculating aquaculture system

    Water treatment components in recirculating aquaculture systems in generally address solids removal, nitrification, circulation, aeration, and degasification. Airlift pumps in a recirculating aquaculture system can address water circulation, aeration, and degasification. Recent data indicates oxygen...

  7. Nutrient flow in improved upland aquaculture systems in Yen Chau, province Son La (Vietnam)

    Pucher, Johannes Gregor

    2014-01-01

    In South-East Asia, pond aquaculture plays an important role in the integrated agriculture aquaculture systems of small-scale farmers and contributes to their food security and income. In mountainous regions, aquaculture differs from aquaculture that is practiced in the lowland due to differences in climate and availability of feeds, fertilizers and water. In Northern Vietnam, the traditional aquaculture is a polyculture of 5-7 fish species. The macro-herbivorous grass carp (Ctenopharyngodon ...

  8. Microorganisms in recirculating aquaculture systems and their management

    Rurangwa, E.; Verdegem, M.C.J.

    2015-01-01

    Recirculation aquaculture systems (RASs) are increasingly considered as production systems of the future with a minimum ecological impact for the production of aquatic food. To maintain a good water quality and to produce quality and healthy fishery products, the systems depend on a diverse microbia

  9. Low-head saltwater recirculating aquaculture systems utilized for juvenile red drum production

    Recirculating aquaculture systems reuse water with mechanical and biological treatment between each use and thus require wastewater treatment techniques for continuous waste removal. However, the traditional techniques and equipment utilized in recirculating aquaculture systems are expensive. The d...

  10. An Inexpensive Recirculating Aquaculture System with Multiple Use Capabilities.

    Scurlock, Gerald Don, Jr.; Cook, S. Bradford; Scurlock, Carrie Ann

    1999-01-01

    Describes the construction of an inexpensive recirculating aquaculture system that can hold up to 46 pounds of fish, invertebrates, and mussels for classroom use. The system is versatile, requires little maintenance, and can be used for both teaching and research purposes. (WRM)

  11. The Presence of Pathogenic Bacteria in Recirculating Aquaculture System Biofilms and their Response to Various Sanitizers

    King, Robin K.

    2001-01-01

    THE PRESENCE OF BACTERIAL PATHOGENS IN BIOFILMS OF RECIRCULATING AQUACULTURE SYSTEMS AND THEIR RESPONSE TO VARIOUS SANITIZERS Robin K. King ABSTRACT Recirculating aquaculture offers a prospect for successful fish farming, but this form of aquaculture presents a great potential for pathogenic microorganisms to become established in the system through the formation of biofilms. Biofilms are capable of forming on all aquaculture system components, incorporating the various microflor...

  12. Fuzzy Logic Controller based on geothermal recirculating aquaculture system

    Hanaa M. Farghally; Doaa M. Atia; Hanaa T. El-madany; Faten H. Fahmy

    2014-01-01

    One of the most common uses of geothermal heat is in recirculation aquaculture systems (RAS) where the water temperature is accurately controlled for optimum growing conditions for sustainable and intensive rearing of marine and freshwater fish. This paper presents a design for RAS rearing tank and brazed heat exchanger to be used with geothermal energy as a source of heating water. The heat losses from the RAS tank are calculated using Geo Heat Center Software. Then a plate type heat exchang...

  13. Solid waste reduction of closed recirculated aquaculture systems by secondary culture of detritivorous organisms

    Bischoff, Adrian A.

    2007-01-01

    Conventional production systems used in aquaculture such as ponds, raceways, net cages or recirculating systems have in common that they release large amounts of feed nutrients either in dissolved or particulate form. The efficient removal of suspended solids is a key factor for the successful operation of recirculating aquaculture systems (RAS). The here presented thesis utilised the solid wastes from modern aquaculture recirculating system for carnivorous fish for the secondary production o...

  14. Purification Performance and Production of a Re-circulating Pond Aquaculture System Based on Paddy Field

    Gu Li; Shi-yang Zhang; Ling Tao; Xiao-li Li; Jing-hua Song; Chun-xue Zhang; Jian-qiang Zhu

    2012-01-01

    Developing improved aquaculture systems with a more efficient use of water and less environmental impact is becoming a crying need. A re-circulating aquaculture system consisting of paddy field and fish pond is a new culture mode due to aquaculture combing with agriculture. The present study focused on the purification capacity of the paddy field on nitrogen, phosphorus and organic matter, the fluctuation trend of water quality conditions during the whole rearing process and the culture effic...

  15. Control methodologies based on geothermal recirculating aquaculture system

    One of the most common uses of geothermal heat is in RAS (recirculation aquaculture systems) where the water temperature is accurately controlled for optimum growing conditions for sustainable and intensive rearing of marine and freshwater fish. This paper presents a design for RAS rearing tank and plate type heat exchanger to be used with geothermal energy as a source of heating water. A well at Umm Huweitat on the Red Sea is used as a source of geothermal energy. The heat losses from the RAS tank are calculated using Geo Heat Center Software. Then a plate type heat exchanger is designed using the epsilon–NTU (number of transfer units) analysis method. For optimal growth and abundance of production, a different techniques of control system are applied to control the water temperature. The total system is built in MATLAB/SIMULINK to study the overall performance of control unit. Finally, a comparison between PI, Fuzzy-PID, and Fuzzy Logic Control has been done. - Highlights: • Design recirculating aquaculture system using geothermal energy. • Design a PI controller for water temperature control. • Design a Fuzzy logic controller for water temperature control. • Design a Fuzzy-PID controller for water temperature control. • Comparison between different control systems

  16. Application of Aquaculture Monitoring System Based on CC2530

    Chen, H. L.; Liu, X. Q.

    In order to improve the intelligent level of aquaculture technology, this paper puts forward a remote wireless monitoring system based on ZigBee technology, GPRS technology and Android mobile phone platform. The system is composed of wireless sensor network (WSN), GPRS module, PC server, and Android client. The WSN was set up by CC2530 chips based on ZigBee protocol, to realize the collection of water quality parameters such as the water level, temperature, PH and dissolved oxygen. The GPRS module realizes remote communication between WSN and PC server. Android client communicates with server to monitor the level of water quality. The PID (proportion, integration, differentiation) control is adopted in the control part, the control commands from the android mobile phone is sent to the server, the server again send it to the lower machine to control the water level regulating valve and increasing oxygen pump. After practical testing to the system in Liyang, Jiangsu province, China, temperature measurement accuracy reaches 0.5°C, PH measurement accuracy reaches 0.3, water level control precision can be controlled within ± 3cm, dissolved oxygen control precision can be controlled within ±0.3 mg/L, all the indexes can meet the requirements, this system is very suitable for aquaculture.

  17. Solar energy system design for a lobster aquaculture facility

    1977-09-30

    In aquaculture, as in most manufacturing processes, the operating cost is greatly dependent upon the cost of energy. The objectives were to: (a) analyze the power requirements for a lobster aquaculture plant, and (b) to evaluate the use of solar energy as a cost reduction measure in plant operation. A flat plate collector system capable of supplying heat alone was compared with a total energy system in which both electrical power and heat were supplied. The flat plate collector was not cost effective because when heat was needed in December, the least amount of heat was available from solar radiation. Therefore, the collector area and cost were prohibitive. However, the total energy system was cost effective when the capital investment was amortized over ten or more years. The optimum solar power plant was designed to provide 100% of the average yearly power demands, or 60% of the December power requirement. This plant would consist of 60,000 square feet of mirror surface (3.5 acres of land for 40% packing density) which would concentrate 1500 to 2000 suns on a receiver mounted on an 85 foot tower. In the tower would be the three storage stoves which would contain the heat required to operate a 343 KWe Brayton gas turbine engine and alternator for 27 hours. Equipment to generate 3 million kw-hr annually will cost an estimated $1.3 million.

  18. Exergetic performance analysis of a recirculating aquaculture system

    Kucuk, Haydar [Mechanical Engineering Department, Guemueshane University, 29000 Guemueshane (Turkey); Midilli, Adnan [Energy Division, Mechanical Engineering Department. Nigde University, 51000 Nigde (Turkey); Oezdemir, Atilla; Cakmak, Eyuep [Central Fisheries Research Institute, Ministry of Agricultural and Rural Affairs, Trabzon (Turkey); Dincer, Ibrahim [Faculty of Engineering and Applied Science, University of Ontario Institute of Technology, 2000 Simcoe Street North, Oshawa, Ont. (Canada)

    2010-05-15

    This paper examines exergetic aspects of a Recirculation Aquaculture System (RAS) for Black Sea trout (Salmo trutta labrax) fingerling rearing at the Trabzon Central Fisheries Research Institute, Turkey. In its thermodynamic analysis, each component of the RAS is treated as a steady-state steady flow system and its exergetic efficiencies are studied. In addition, the following parameters are measured and recorded in experiments: the mass flow rates, inlet and outlet temperatures and of the system components, surrounding temperatures, and electrical work utilized by the components in the RAS. Based on these experimental data, inlet and outlet exergy values, exergy losses, and exergetic efficiencies of each component in the system are determined to assess their performance. Moreover, the overall system exergy efficiency is determined. The results show that exergy efficiencies of the system components are highly affected by varying input exergy flows as a function of the surrounding temperature and chiller's operating period. (author)

  19. Life Cycle Assessment for environmentally sustainable aquaculture management : a case study of combined aquaculture systems for carp and tilapia

    Mungkung, R.; Aubin, J.; Prihadi, T. H.; Slernbrouck, J.; van der Werf, H. M. G.; Legendre, Marc

    2013-01-01

    Life Cycle Assessment (LCA) was applied to evaluate the potential environmental impacts associated with two-net cage aquaculture systems of common carp (Cyprinus carpio carpio) and tilapia (Oreochromis niloticus) in the Cirata reservoir, Indonesia. The studied system included fingerling production in hatcheries, fish rearing in cages, and transport of fry and feed as well as that of harvested fish to markets. The environmental impact indicators were calculated based on the annual production i...

  20. The Use of Vision in a Sustainable Aquaculture Feeding System

    Jer-Vui Lee

    2013-10-01

    Full Text Available Dairy feeding causes significant water pollution. By controlling the proper amount of feed, reducing the waste to minimum will effectively reduce the problem of water contamination. In this project, a Sustainable Aquaculture Feed System (SAFS has been designed and developed. It can automatically feed the fishes by estimating fishes’ appetite through machine vision. The discussion includes design and optimization of the vision system using Labview as well as the integration of various components in the SAFS. With the developed algorithm, the system is able to detect the presence of fishes and count the number of fishes. The outcome is able to estimate and infer the fish appetite. Therefore, the feeding time can be planned ahead. In addition, the system includes a Graphical User Interface (GUI for monitoring, display the feeding status and sensors reading such as pH, turbidity and temperature.

  1. Microbial diversity of biological filters in recirculating aquaculture systems.

    Schreier, Harold J; Mirzoyan, Natella; Saito, Keiko

    2010-06-01

    Development of environmentally sustainable farming of marine and freshwater species using recirculating aquaculture systems (RASs) requires a complete understanding of the biological component involved in wastewater treatment. This component integrates biofilters composed of microbial communities whose structure, dynamics, and activities are responsible for system success. Engineering highly efficient, environmentally sound, disease-free, and economically viable systems necessitates a thorough knowledge of microbial processes involved in all facets of RAS biofilters and has only recently been the focus of comprehensive studies. These studies have included the application of molecular tools to characterize community diversity and have identified key processes useful for improving system performance. In this paper we summarize the current understanding of the microbial diversity and physiology of RAS biofilters and discuss directions for future studies. PMID:20371171

  2. Fuzzy Logic Controller based on geothermal recirculating aquaculture system

    Hanaa M. Farghally

    2014-01-01

    Full Text Available One of the most common uses of geothermal heat is in recirculation aquaculture systems (RAS where the water temperature is accurately controlled for optimum growing conditions for sustainable and intensive rearing of marine and freshwater fish. This paper presents a design for RAS rearing tank and brazed heat exchanger to be used with geothermal energy as a source of heating water. The heat losses from the RAS tank are calculated using Geo Heat Center Software. Then a plate type heat exchanger is designed using the epsilon – NTU analysis method. For optimal growth and abundance of production, a Fuzzy Logic control (FLC system is applied to control the water temperature (29 °C. A FLC system has several advantages over conventional techniques; relatively simple, fast, adaptive, and its response is better and faster at all atmospheric conditions. Finally, the total system is built in MATLAB/SIMULINK to study the overall performance of control unit.

  3. Life cycle assessment of food production in integrated agriculture–aquaculture systems of the Mekong Delta

    Phong, L.T.; Boer, de I.J.M.; Udo, H.M.J.

    2011-01-01

    This study evaluated the environmental impact of integrated agriculture–aquaculture (IAA) farming systems in the Mekong Delta that differ in types of aquaculture intensification. Daily inputs and outputs for rice, fruits, vegetables, pigs, poultry, and fish were collected on 11 farms over a period o

  4. Low-head recirculating aquaculture system for juvenile red drum production

    The USDA Agricultural Research Service and the Center for Aquaculture and Stock Enhancement at Harbor Branch Oceanographic Institute-FAU (HBOI-FAU) are collaborating to evaluate low-head recirculating aquaculture system designs to intensively produce red drum juveniles as part of the Florida Fish an...

  5. Aquaculture Industry Potential and Issues: A Case from Cage Culture System Entrepreneurs: Suggestions for Intensification of Aquaculture Industry

    A. N.A. Faiz

    2010-01-01

    Full Text Available Problem statement: Cage culture has become a popular aquaculture system nowadays. In the recent statistics provided by DOF, the cage culture system has generated almost USD 400 m (RM 1.39 b of income per year. In order to further intensify this economic activity the Ministry of Agriculture and Agro-based Industry (MOA through the DOF has zoned the aquaculture industry via Aquaculture Industry Zone (ZIA Programs throughout Malaysia. Cage culture system seems to have the ability to be a catalyst in enhancing the economy level of local people but it is well known that certain problems must be overcome first before the potential can be clearly seen. Thus the main focus of this study is to clarify all the potentials and problems faced by the cage culture system entrepreneurs in running their aquaculture activity. Approach: The research approach for this study was a qualitative case study that provided an in-depth description of potentials and issues in aquaculture industry in the district of Kuala Pahang, Malaysia. Data was gained using a Focus Group Discussion (FGD among the cage culture system entrepreneurs guided by an interview guide. A total of 10 cage culture entrepreneurs were selected as the FGD members. The questions served as a guide, but allowed respondents freedom and flexibility in their answers. The findings were in descriptive analysis. Results: The cage culture system was found to provide better income that lead to a higher quality of life for the entrepreneurs, positive intangible values existed among the entrepreneurs; experiences have taught them to become self independent. There were problems identified such as no independence in selecting the fingerlings, environmental problems seem to burden them, unstable pellet price, no official agreement between them and the government emphasizing the portion of the river is given to them for running their business and lot of bureaucracies that the entrepreneurs need to face. Conclusion

  6. Microscreen effects on water quality in replicated recirculating aquaculture systems

    Fernandes, Paulo; Pedersen, Lars-Flemming; Pedersen, Per Bovbjerg

    2015-01-01

    This study investigated the effects of three microscreen mesh sizes (100, 60 and 20 μm) on water quality and rainbow trout (Oncorhynchus mykiss) performance compared to a control group without microscreens, in triplicated recirculating aquaculture systems (RAS). Operational conditions were kept....... Fish performed similarly in all treatments. Preliminary screening of trout gills did not reveal any pathological changes related to microscreen filtration and the resulting water quality. Biofilter performance was also unaffected, with 0′-order nitrification rates (k0a) being equivalent for all twelve...... increased β-values. Particulate parameters reached steady-state in all treatment groups having microscreens at the end of the trial. The time to reach equilibrium seemingly increased with increasing mesh size but the three treatment groups (100, 60 and 20 μm) did not significantly differ at the end of the...

  7. The evaluation of oxygen and carbon dioxide transfer associated with airlifts in recirculating aquaculture systems

    Airlifts in recirculating aquaculture systems (RAS) provide aeration, degasification, and water circulation. They allow the simplification of systems, and if designed properly, can reduce the capital costs and minimize operation and maintenance associated with alternative pumping systems. In order t...

  8. Effect of Wind-solar Complementary Increasing Oxygen System in Aquaculture

    Guodong You

    2013-12-01

    Full Text Available In order to explore a sustainable aquaculture methodology, water was increased oxygen by a wind-solar power which was tested in a production period of the Penaeus vannamei in paper. The result shows that, compareing with the conventional aerating comparison, the system could improve water environment in ponds and dissolved oxygen, temperature, pH value and the content of nitrite stably and uniformly change; make full use of wind energy and solar energy clean energy, save 100% on power consumption, 15.9% on bait, 46.0% on drug; save aquaculture costs, increase income 282%. Using "Wind-solar" complementary increasing oxygen can improve water environment, save power, reduce aquaculture costs, increase aquaculture production and income, provide a new thought and method for green and ecological aquaculture, so it has high value of practical application.

  9. Risk Analysis of Tilapia Recirculating Aquaculture Systems: A Monte Carlo Simulation Approach

    Kodra, Bledar

    2007-01-01

    Risk Analysis of Tilapia Recirculating Aquaculture Systems: A Monte Carlo Simulation Approach Bledar Kodra (ABSTRACT) The purpose of this study is to modify an existing static analytical model developed for a Re-circulating Aquaculture Systems through incorporation of risk considerations to evaluate the economic viability of the system. In addition the objective of this analysis is to provide a well documented risk based analytical system so that individuals (investors/lenders) c...

  10. Dietary carbohydrate composition can change waste production and biofilter load in recirculating aquaculture systems

    Meriac, A.; Eding, E.H.; Schrama, J.W.; Kamstra, A.; Verreth, J.A.J.

    2014-01-01

    This study investigated the effect of dietary carbohydrate composition on the production, recovery and degradability of fecal waste from rainbow trout (Oncorhynchus mykiss) in recirculating aquaculture systems (RAS). Dietary carbohydrate composition was altered by substituting starch with non-starch

  11. Use of planted biofilters in integrated recirculating aquaculture-hydroponics systems in the Mekong Delta, Vietnam

    Trang, N.T.D.; Brix, Hans

    2014-01-01

    The feasibility of using planted biofilters for purification of recirculated aquaculture water in the Mekong Delta of Vietnam was assessed. The plant trenches were able to clean tilapia aquaculture water and to maintain good water quality in the fish tanks without renewal of the water. NH4-N was ...... rates of 725 kg N and 234 kg P ha-1 year-1. This research demonstrates that integrated recirculating aquaculture-hydroponics (aquaponics) systems provide significant water savings and nutrient recycling as compared with traditional fish ponds. © 2012 John Wiley & Sons Ltd....

  12. New developments in recirculating aquaculture systems in Europe: A perspective on environmental sustainability

    Martins, C. I. M.; Eding, E.H.; Verdegem, M.C.J.; Heinsbroek, L.T.N.; Schneider, O.(Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland); Blancheton, Jean-Paul; Roque d'Orbcastel, Emmanuelle; Verreth, J.A.J.

    2010-01-01

    The dual objective of sustainable aquaculture, i.e., to produce food while sustaining natural resources is achieved only when production systems with a minimum ecological impact are used. Recirculating aquaculture systems (RASs) provide opportunities to reduce water usage and to improve waste management and nutrient recycling. RAS makes intensive fish production compatible with environmental sustainability. This review aims to summarize the most recent developments within RAS that have contri...

  13. Nutrient fate in aquacultural systems for waste treatment

    Dontje, J.H.; Clanton, C.J.

    1999-08-01

    Twelve small, recirculating aquacultural systems were operated for livestock waste treatment to determine nutrient fate. Each system consisted of a 730-L fish tank coupled in a recirculating loop with three sand beds (serving as biofilters) in parallel. Fish (Tilapia species) were grown in the tanks while cattails, reed canary grass, and tomatoes were grown in separate sand beds. Swine waste was added to the fish tanks every other day at average rates of 50, 72, 95, and 118 kg-COD/ha/day of fish tank surface (three replications of each loading rate). Water from the fish tanks was filtered through the sand beds three times per day with 20% of the tank volume passing through the sand each day. The systems were operated in a greenhouse for eight months (21 July to 8 March). Aboveground plant matter was harvested at eight-week intervals. The fish were removed after four months and the tanks were restocked with fingerlings. Initial and final nitrogen (N), phosphorus (P), and potassium (K) contents of the system components, as well as that of the harvested plants and fish, were determined. Nutrient balance calculations revealed that 30 to 68% of added N was lost from the systems, probably via denitrification. Nutrient removal by plants was 6 to 18% for N, 8 to 21% for P, and 25 to 71% for K, with tomatoes (foliage and fruit) accounting for the majority of the removal. Plant growth was limited by growing conditions (particularly day length), not be nutrient availability. Fish growth was limited by temperature; thus nutrient extraction by the fish was minimal. Under the conditions of this experiment, the system required supplemental aeration.

  14. LCA and emergy accounting of aquaculture systems: towards ecological intensification.

    Wilfart, Aurélie; Prudhomme, Jehane; Blancheton, Jean-Paul; Aubin, Joël

    2013-05-30

    An integrated approach is required to optimise fish farming systems by maximising output while minimising their negative environmental impacts. We developed a holistic approach to assess the environmental performances by combining two methods based on energetic and physical flow analysis. Life Cycle Assessment (LCA) is a normalised method that estimates resource use and potential impacts throughout a product's life cycle. Emergy Accounting (EA) refers the amount of energy directly or indirectly required by a product or a service. The combination of these two methods was used to evaluate the environmental impacts of three contrasting fish-farming systems: a farm producing salmon in a recirculating system (RSF), a semi-extensive polyculture pond (PF1) and an extensive polyculture pond (PF2). The RSF system, with a low feed-conversion ratio (FCR = 0.95), had lower environmental impacts per tonne of live fish produced than did the two pond farms, when the effects on climate change, acidification, total cumulative energy demand, land competition and water dependence were considered. However, RSF was clearly disconnected from the surrounding environment and depended highly on external resources (e.g. nutrients, energy). Ponds adequately incorporated renewable natural resources but had higher environmental impacts due to incomplete use of external inputs. This study highlighted key factors necessary for the successful ecological intensification of fish farming, i.e., minimise external inputs, lower the FCR, and increase the use of renewable resources from the surrounding environment. The combination of LCA and EA seems to be a practical approach to address the complexity of optimising biophysical efficiency in aquaculture systems. PMID:23531606

  15. Feasibility of adopting aquaculture without detriment to existing farming practices: a case of Bangladesh farming systems

    Ahmed, M.; Rab, M.A.

    1992-01-01

    Integrating agriculture aquaculture that would draw inputs from on farm sources is viewed as a viable option to improve the productivity, income and resource use efficiency of existing farms in Bangladesh. To assess the existing resource availability, use pattern and efficiency before introducing new aquaculture technology within the existing farm systems, a survey of 330 pond operating farm households was conducted in six selected unions from two thanas (subdistricts) of Bangladesh.

  16. The economic sustainability of land-based aquaculture systems: An integrated analysis

    Bennich, Therese

    2015-01-01

    The global demand for fish is rising, caused by population growth and an increasing per capita consumption of fish. At the same time, production from capture fisheries has stagnated and there are growing concerns about the environmental impact of sea-based aquaculture. Future developments in the fishing industry must meet demand, without compromising the environment. Technological advancements have introduced land-based fish farming and Recirculating Aquaculture Systems (RAS) technology as a ...

  17. Multidrug-Resistance and Toxic Metal Tolerance of Medically Important Bacteria Isolated from an Aquaculture System

    Resende, Juliana Alves; Silva, Vânia L.; Fontes, Cláudia Oliveira; Souza-Filho, Job Alves; de Oliveira, Tamara Lopes Rocha; Coelho, Cíntia Marques; César, Dionéia Evangelista; Diniz, Cláudio Galuppo

    2012-01-01

    The use of antimicrobials and toxic metals should be considered carefully in aquaculture and surrounding environments. We aimed to evaluate medically relevant bacteria in an aquaculture system and their susceptibility to antimicrobials and toxic metals. Selective cultures for enterobacteria (ENT), non-fermenting Gram-negative rods (NFR) and Gram-positive cocci (GPC) were obtained from water samples collected in two different year seasons. The isolated bacteria were biochemically identified an...

  18. Influence of carbohydrate addition on nitrogen transformations and greenhouse gas emissions of intensive aquaculture system.

    Hu, Zhen; Lee, Jae Woo; Chandran, Kartik; Kim, Sungpyo; Sharma, Keshab; Khanal, Samir Kumar

    2014-02-01

    Aquaculture is one of the fastest-growing segments of the food economy in modern times. It is also being considered as an important source of greenhouse gas (GHG) emissions. To date, limited studies have been conducted on GHG emissions from aquaculture system. In this study, daily addition of fish feed and soluble starch at a carbon-to-nitrogen (C/N) ratio of 16:1 (w/w) was used to examine the effects of carbohydrate addition on nitrogen transformations and GHG emissions in a zero-water exchange intensive aquaculture system. The addition of soluble starch stimulated heterotrophic bacterial growth and denitrification, which led to lower total ammonia nitrogen, nitrite and nitrate concentrations in aqueous phase. About 76.2% of the nitrogen output was emitted in the form of gaseous nitrogen (i.e., N2 and N2O) in the treatment tank (i.e., aquaculture tank with soluble starch addition), while gaseous nitrogen accounted for 33.3% of the nitrogen output in the control tank (i.e., aquaculture tank without soluble starch addition). Although soluble starch addition reduced daily N2O emissions by 83.4%, it resulted in an increase of daily carbon dioxide (CO2) emissions by 91.1%. Overall, starch addition did not contribute to controlling the GHG emissions from the aquaculture system. PMID:24140689

  19. Application of airlift technology in recirculation aquaculture systems

    Marine hatcheries implementing recirculating aquaculture technology require pristine water quality and must be designed to provide a disease free environment as much as possible to limit disease transmission. Given the aggressive nature of a variety of marine pathogens, design considerations with re...

  20. Aquaculture; Acquacoltura

    De Murtas, I.D. [ENEA, Centro Ricerche Casaccia, Rome (Italy). Dipt. Ambiente

    1998-12-01

    This paper attempts an overview of the progress made in the field of aquaculture. Aquaculture is a system of techniques strongly influenced by natural environmental conditions. Aquaculture as a biological technique oriented towards the production of useful aquatic organisms, is reaching a stage of consolidation which will place it on an equal footing which agriculture and animal husbandry. Aquaculture provides important economic and nutritional benefits to many regions of developing world. In 1994, over 90 percent of total aquaculture production was in Asia, with China, India, Japan, Indonesia, Thailand, Philippines and Republic of Korea as the seven leader producers. [Italiano] L`acquacoltura, vale a dire l`arte di riprodurre artificialmente pesci, alghe, molluschi e crostacei ed altri organismi acquatici utili all`uomo, si presenta oggi come un`attivita` di assoluto rilievo nell`insieme dei vari comparti di produzione alimentare. L`aumento della produzione e` costante anche se cinque paesi asiatici (Cina, India, Giappone, Filippine e Corea del Sud) contribuiscono per l`80% al volume della produzione mondiale. Nel presente lavoro vengono descritti lo stato dell`acquacoltura e della maricoltura nel mondo e le filiere di allevamento delle principali specie.

  1. Implications of Extracellular Polymeric Substance Matrices of Microbial Habitats Associated with Coastal Aquaculture Systems

    Juan Carlos Camacho-Chab

    2016-08-01

    Full Text Available Coastal zones support fisheries that provide food for humans and feed for animals. The decline of fisheries worldwide has fostered the development of aquaculture. Recent research has shown that extracellular polymeric substances (EPS synthesized by microorganisms contribute to sustainable aquaculture production, providing feed to the cultured species, removing waste and contributing to the hygiene of closed systems. As ubiquitous components of coastal microbial habitats at the air–seawater and seawater–sediment interfaces as well as of biofilms and microbial aggregates, EPS mediate deleterious processes that affect the performance and productivity of aquaculture facilities, including biofouling of marine cages, bioaccumulation and transport of pollutants. These biomolecules may also contribute to the persistence of harmful algal blooms (HABs and their impact on cultured species. EPS may also exert a positive influence on aquaculture activity by enhancing the settling of aquaculturally valuable larvae and treating wastes in bioflocculation processes. EPS display properties that may have biotechnological applications in the aquaculture industry as antiviral agents and immunostimulants and as a novel source of antifouling bioproducts.

  2. Long term/low dose formalin exposure to small-scale recirculation aquaculture systems

    Pedersen, Lars-Flemming; Pedersen, Per Bovbjerg; Nielsen, Jeppe L.;

    2010-01-01

    Repetitive long term formalin application at low dose was investigated to determine the effect on formaldehyde removal rate, biofilter nitrification and the microbial composition in small-scale recirculation aquaculture biofilters. Six pilot-scale recirculation aquaculture systems holding rainbow.......9%, whereas Nitrobacter sp. was not detected. The relative abundances of AOB and NOB in the untreated system were generally higher compared to the system exposed to formalin. Low dose formalin in recirculated aquaculture systems proved to be a possible treatment strategy, as the effect on nitrification was...... were positively correlated to the amount and frequency of formalin treatment. In systems with regularly low formalin dosage, the formaldehyde removal rate increased up to tenfold from 0.19 +/- 0.05 to 1.81 +/- 0.13 mg/(L h). Biofilter nitrification was not impaired in systems treated with formalin on a...

  3. Dietary carbohydrates and denitrification in recirculating aquaculture systems

    Meriac, A.

    2014-01-01

    Due to overfishing of global fish stocks and increasing fish meal prices, plant ingredients are being increasingly used as an alternative source of protein in fish feeds. However, the inclusion of unpurified plant ingredients will also increase the content of fibers in feeds. Fibers are nearly indigestible and will therefore increase solid waste production in aquaculture. This solid waste can be used to as a carbon source for denitrification to control nitrate levels in recirculating aquacult...

  4. Ornithinimicrobium tianjinense sp. nov., isolated from a recirculating aquaculture system.

    Liu, Liang-Zi; Liu, Ying; Chen, Zhu; Liu, Hong-Can; Zhou, Yu-Guang; Liu, Zhi-Pei

    2013-12-01

    A Gram-positive, strictly aerobic and heterotrophic, non-spore-forming actinobacterium (strain B2(T)) isolated from a recirculating aquaculture system was studied for its taxonomic position. Strain B2(T) formed a rudimentary substrate-mycelium that fragmented into short rod-shaped to coccoid cells (0.5 µm×0.5-2.2 µm or 0.5-1.0 µm in diameter). Colonies were yellow, smooth, circular and 1.5-2.0 mm in diameter after incubation on TSA for 3 days at 30 °C. Strain B2(T) grew at 20-40 °C (optimal, 30 °C) and pH 5.5-9.5 (optimal, 6.5-7.0) and in the presence of 0-9% (w/v) NaCl (optimal, 1%). The predominant menaquinone of strain B2(T) was MK-8(H4). The cell-wall peptidoglycan of strain B2(T) contained the amino acids ornithine, glutamic acid, alanine, glycine and aspartic acid. The major polar lipids were phosphatidylglycerol and diphosphatidylglycerol. The major fatty acids were iso-C15 : 0, iso-C16 : 0 and summed feature 9. Its DNA G+C content was 68.3 mol% (Tm). Analysis of 16S rRNA gene sequences indicated that strain B2(T) was related phylogenetically to members of the genus Ornithinimicrobium with highest similarity (96.6 %) to Ornithinimicrobium kibberense DSM 17687(T), followed by Ornithinimicrobium humiphilum DSM 12362(T) (96.3 %), Ornithinimicrobium pekingense LW6(T) (96.2%) and Ornithinimicrobium murale 01-Gi-040(T) (94.8%). On basis of phenotypic, chemotaxonomic and phylogenetic data, it was concluded that strain B2(T) represents a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium tianjinense sp. nov. is proposed. The type strain is B2(T) ( =CGMCC 1.12160(T) =JCM 18464(T)). PMID:23907216

  5. Description and Improvement of the 'Whedo'-Aquaculture-System in Malanville (North of Benin)

    Hauber, Melanie Erika

    2011-01-01

    This work delves into the recently developed ‘Whedo’-aquaculture-system in the rural community of Malanville (North Benin)and aims on providing a closer insight on this – for the area--recent system including the ecological but also the sociological and economical aspects in order to develop this extensive traditional fishery to a more productive semi-intensive aquaculture system. With the retreat of the flood ‘Whedos’ usually become infested with numerous hydato-and tenagophytes, while the p...

  6. Measuring System for Growth Control of the Spirulina Aquaculture

    Ponce S., Claudio; Ponce L., Ernesto; Bernardo S., Barraza

    2008-11-01

    It describes the workings of a data-logging instrument that measures growth levels of the Spirulina aquaculture. The Spirulina is a very delicate algae and its culture may be suddenly lost due to overgrowth. This kind of instrument is not at present available in the market. The transduction is a submergible laser device whose measuring margin of error is near to 0.28%. The advantage of this new instrument is the improvement in the measurement and the low cost. The future application of this work is related to the industrial production of food and fuel from micro algae culture, for the growing world population.

  7. Development of a seaweed species-selection index for successful culture in a seaweed-based integrated aquaculture system

    Kang, Yun Hee; Hwang, Jae Ran; Chung, Ik Kyo; Park, Sang Rul

    2013-03-01

    Integrated multi-trophic aquaculture (IMTA) has been proposed as a concept that combines the cultivation of fed aquaculture species ( e.g., finfish/shrimp) with extractive aquaculture species ( e.g., shellfish/seaweed). In seaweed-based integrated aquaculture, seaweeds have the capacity to reduce the environmental impact of nitrogen-rich effluents on coastal ecosystems. Thus, selection of optimal species for such aquaculture is of great importance. The present study aimed to develop a seaweed species-selection index for selecting suitable species in seaweed-based integrated aquaculture system. The index was synthesized using available literature-based information, reference data, and physiological seaweed experiments to identify and prioritize the desired species. Undaria pinnatifida, Porphyra yezoensis and Ulva compressa scored the highest according to a seaweed-based integrated aquaculture suitability index (SASI). Seaweed species with the highest scores were adjudged to fit the integrated aquaculture systems. Despite the application of this model limited by local aquaculture environment, it is considered to be a useful tool for selecting seaweed species in IMTA.

  8. The accumulation of substances in Recirculating Aquaculture Systems (RAS) affects embryonic and larval development in common carp Cyprinus carpio

    Martins, C.I.; Pristin, M.G.; Ende, S.S.W.; Eding, E.H.; Verreth, J.A.J.

    2009-01-01

    The accumulation of substances in Recirculating Aquaculture Systems (RAS) may impair the growth and welfare of fish. To test the severity of contaminants accumulated in RAS, early-life stages of fish were used. Ultrafiltered water from two Recirculating Aquaculture Systems (RAS), one RAS with a high

  9. Flow, aeration, and carbon dioxide transfer rates for airlifts used in recirculating aquaculture systems

    Airlift pumping systems reduce the electrical costs of moving water in a recirculating aquaculture system and can be concurrently designed to aerate water and remove carbon dioxide. This study determined the water flow, oxygen transfer, and CO2 removal rates for water using airlift technology in a 1...

  10. Nitrate removal effectiveness of fluidized sulfur-based autotrophic denitrification biofilters for recirculating aquaculture systems

    There is a need to develop practical methods to reduce nitrate -nitrogen loads from recirculating aqua-culture systems to facilitate increased food protein production simultaneously with attainment of water quality goals. The most common wastewater denitrification treatment systems utilize methanol-...

  11. End-of-pipe single-sludge denitrification in pilot-scale recirculating aquaculture systems

    Suhr, Karin Isabel; Pedersen, Lars-Flemming; Nielsen, J.L.

    2014-01-01

    A step toward environmental sustainability of recirculat aquaculture systems (RAS) is implementation ofsingle-sludge denitrification, a process eliminating nitrate from the aqueous environment while reduc-ing the organic matter discharge simultaneously. Two 1700 L pilot-scale RAS systems each wit...

  12. Comparison of phytoplankton communities in catfish split-pond aquaculture systems with conventional ponds.

    There has been a growing interest and use of variations of partitioned aquaculture systems (PAS) in recent years by the southeastern United States of America farmed catfish industry. Split-pond systems, one type of PAS, are designed to better manage fish waste byproducts (e.g., ammonia) and dissolv...

  13. Fate of water borne therapeutic agents and associated effects on nitrifying biofilters in recirculating aquaculture systems

    Pedersen, Lars-Flemming

    of these agents on biofilter nitrification performance. All experiments were conducted through addition of chemical additives to closed pilot scale recirculating aquaculture systems (RAS) with fixed media submerged biofilters under controlled operating conditions with rainbow trout (Oncorhynchus mykiss......) in a factorial design with true replicates. Biofilter nitrification performances were evaluated by changes in chemical processes, and nitrifying populations were identified by fluorescence in situ hybridisation (FISH) analysis. FA was degraded at a constant rate immediately after addition, and found......Recent discharge restrictions on antibiotics and chemotherapeutant residuals used in aquaculture have several implications to the aquaculture industry. Better management practices have to be adopted, and documentation and further knowledge of the chemical fate is required for proper administration...

  14. Microbial communities of recirculating aquaculture facilities: interaction between heterotrophic and autotrophic bacteria and the system itself

    Michaud, Luigi

    2007-01-01

    Les systèmes d'aquaculture en circuit recirculé (Recirculating Aquaculture Systems, RAS) peuvent être considérés comme une alternative à la technologie de l'aquaculture en milieu ouvert et en bassins (en consommant moins d'eau pour un même rendement de production) ou peuvent être intégrés dans une chaîne de production avec des systèmes d'enclos en filet. L'intérêt des RAS est dû à leurs avantages intrinsèques comme la réduction des besoins en surface et en eau, le haut niveau de contrôle e...

  15. Recent advances within intensive Recirculated Aquaculture System cultivation of the calanoid copepod Acartia tonsa (Dana)

    Jepsen, Per Meyer; Højgaard, Jacob Kring; Drillet, Guillaume;

    2014-01-01

    interesting trait with the species is that the eggs can be provoked into a resting stage, where the egg can be stored for one year, similar to Artemia cysts. This is the most promising storage technique for distribution of copepod eggs to aquaculture facilities worldwide. The eggs can be hatched and the...... nauplii can be feed to marine fish larvae. A restriction is that copepod cultures for producing eggs are after 30 years of research still not stable and in large enough scale for bulk production of eggs. Recently a unique copepod Recirculated Aquaculture System (RAS) at Roskilde University (Denmark) was...... constructed as a part of the IMPAQ project “IMProvement of AQuaculture high quality fish fry production”. We present recent advance within RAS culture for copepods, and lesson learned from rearing the specie. Further we present physical and biological culture restrictions in terms of water quality (NH3...

  16. Electronics and electronic systems

    Olsen, George H

    1987-01-01

    Electronics and Electronic Systems explores the significant developments in the field of electronics and electronic devices. This book is organized into three parts encompassing 11 chapters that discuss the fundamental circuit theory and the principles of analog and digital electronics. This book deals first with the passive components of electronic systems, such as resistors, capacitors, and inductors. These topics are followed by a discussion on the analysis of electronic circuits, which involves three ways, namely, the actual circuit, graphical techniques, and rule of thumb. The remaining p

  17. Mass transfer coefficients for carbon dioxide and oxygen for a polygeyser biofilter using airlift in an aquaculture reuse system

    Aquaculture is a rapidly growing industry, accounting for over one-third of all direct fisheries consumption. In recirculating aquaculture systems the primary technology being used is energy intensive because water must be moved continuously through the culture and treatment units to remove wastes ...

  18. BIOFILTERS IN AQUACULTURE

    Ivo Šarić; Marina Barilo; Ana Gavrilović; Jurica Jug-Dujaković

    2010-01-01

    Recirculating aquaculture is one of the solutions to an environmentally sustainable and economically feasible aquaculture production, and can be established in either urban or rural communities. Controlled conditions enable optimal growth conditions suitable for the species in question during the whole growth process, as well as the shortening of the process itself, when compared to open uncontrolled systems. Recirculating systems are now one of the most researched fields of aquaculture, and ...

  19. Particle sieve analysis for solids removal efficiency for recirculating aquaculture system components

    As the aquaculture industry develops, opportunites for small-scale farms to become an integral part of the industry will become more prevalent. For small-scale operations it is important to have a system design that is efficient in water and energy use. If such operations decide to utilize recircula...

  20. Membrane biological reactor treatment of a saline backwash flow from a recirculating aquaculture system

    A recirculating aquaculture system (RAS) can minimize water use, allowing fish production in regions where water is scarce and also placing the waterborne wastes into a concentrated and relatively small volume of effluent. The RAS effluent generated during clarifier backwash is usually small in vol...

  1. Optimizing hydraulic retention times in denitrifying woodchip bioreactors treating recirculating aquaculture system wastewater

    The performance of wood-based denitrifying bioreactors to treat high-nitrate wastewaters from aquaculture systems has not previously been demonstrated. Four pilot-scale woodchip bioreactors (approximately 1:10 scale) were constructed and operated for 268 d to determine the optimal range of design hy...

  2. Observations on side-swimming rainbow trout Oncorhynchus mykiss in water recirculation aquaculture systems

    During a controlled 6-month study using six replicated water recirculation aquaculture systems (WRAS), it was observed that rainbow trout Oncorhynchus mykiss in all WRAS exhibited a higher-than-normal prevalence of side-swimming (i.e. controlled, forward swimming, but with misaligned orientation suc...

  3. Disinfection of water in recirculating aquaculture systems with peracetic acid (PAA)

    The disinfection behaviour of peracetic acid (PAA) in recirculating aquaculture systems (RAS) was investigated. Peracetic acid is a strong oxidizing agent found in various concentrations in different products. Three Wofasteril PAA products (E400 (c), Lspecical; AC 150) were tested in vitro for the...

  4. Geosmin causes off-flavour in arctic charr in recirculating aquaculture systems

    The “earthy” and “muddy” off-flavors in pond-reared fish are due to the presence of geosmin or 2-methylisoborneol (MIB) in the flesh of the fish. Similar off-flavors have been reported in fish raised in recirculating aquaculture systems (RAS); however, little information is available regarding the ...

  5. Practical design and application of airlift technology for aquaculture reuse systems

    Air-lift technology to support water movement in low-head water treatment units for recirculating aquacultures systems provides an energy efficient alternative to the use of centrifugal pumps. The benefits of air-lift are further realized when electrical requirements for aeration, CO2 degassing, and...

  6. Performance evaluation of pumping systems used in commercial-scale, split-pond aquaculture

    Split-pond aquaculture systems have been adopted widely by United States catfish farmers as a way to improve production performance. The split-pond consists of a fish-culture basin that is connected to a waste-treatment lagoon by two water conveyance structures. Water is circulated between the two b...

  7. ISO 14001 Environmental Management System (EMS) and Risk Assessment Application in Aquaculture

    ATAYETER, Servet

    2014-01-01

    It is obvious that there is a strong pressure tion. In today's world, companies have to on aquaculture farms for their environmental decrease their negative impacts and show their impact on aquatic environments. Consumers commitment to the environment. An effective are also becoming more and more conscious environmental management system can help a about environmental issues and clean produccompany as an administrative tool.

  8. Strategies to enhance the competitiveness of semi-intensive aquaculture systems in costal earth ponds: the organic aquaculture approach

    Manuel Sardinha

    2014-06-01

    on the performance of seabream. Lower culture density (0.5 kg/m3 resulted in higher overall growth performance. The impact, in terms of nitrogenous (N and phosphorus (P loads, among the various scenarios were calculated and clearly reinforce the environmental sustainability of these semi-intensive production systems. The use of organic feeds does not lead to a significant enhancement of the growth performance. Therefore, the additional costs associated to feeding under organic standards, need to be incorporated in the sale price, thus requiring a targeted and differentiated marketing and distribution approach. Sociedade Piscicultura Farense Lda, as other traditional aquacultures depends on the enhancement of the natural environment, combined with production fine-tuning and product positioning, rewarding the quality of the final product. The positive effects of extensive and semi-intensive aquaculture in coastal areas, including environmental protection and restoration in areas of particular ecological interest, employment opportunity and development in rural and coastal areas are highly appealing concepts for the general community, particularly to conscious consumers.

  9. Purification Performance and Production of a Re-circulating Pond Aquaculture System Based on Paddy Field

    Gu Li

    2012-10-01

    Full Text Available Developing improved aquaculture systems with a more efficient use of water and less environmental impact is becoming a crying need. A re-circulating aquaculture system consisting of paddy field and fish pond is a new culture mode due to aquaculture combing with agriculture. The present study focused on the purification capacity of the paddy field on nitrogen, phosphorus and organic matter, the fluctuation trend of water quality conditions during the whole rearing process and the culture efficacy of the main culture species of grass carp (Ctenopharyngodon idella. The results were as follows: under a flow rate of 1.4-5.5 m3/h for the recirculation treatment, the average removal rate of ammonia nitrogen, nitrate nitrogen, total nitrogen, total phosphorus and biochemical oxygen demand for the aquaculture effluent amounted to 40.5, 43.5, 31.9, 23.9, 20.7 and 52.4%, respectively, But the dissolved oxygen content in the rice fields increased obviously. During the whole process of fish rearing, the main physicochemical parameters of water quality for the experimental ponds were all maintained at a suitable level for the growth of the grass carp. In addition, there were significant differences (p<0.05 in DO, TSS, NH4+ -N, NO--N, BOD5 and Chl-&alpha between the experimental and control ponds. As far as the yield per unit and survival rate was concerned, the level of the experimental ponds was obviously higher than that of the control, while the feed conversion ratio displayed the opposite trend. Overall, the new aquaculture system realized the double aims of water reuse and the reduction of waste water discharge.

  10. An integrated closed system for fish-plankton aquaculture in Amazonian fresh water

    Gilles, Sylvain; Ismino, R.; Sanchez, H.; David, F.; Nunez Rodriguez, Jesus; Dugué, Rémi; Darias, Maria Jose; Römer, U.

    2014-01-01

    A prototype of an integrated closed system for fish-plankton aquaculture was developed in Iquitos (Peruvian Amazonia) in order to cultivate the Tiger Catfish, Pseudoplatystoma punctifer (Castelnau, 1855). This freshwater recirculating system consisted of two linked sewage tanks with an intensive rearing unit (a cage) for P. punctifer placed in the first, and with a fish-plankton trophic chain replacing the filters commonly used in clear water closed systems. Detritivorous and zooplan...

  11. Water chemistry characterization and component performance of a recirculating aquaculture system producing hybrid striped bass

    Easter, Christopher

    1992-01-01

    Eight identical and independent pilot scale recirculating aquaculture production systems were populated with fingerling hybrid striped bass (Morone chrysops female x Morone saxatilis male). Three population densities were established with two replicates at 132 fishlm3 and three replicates each at 66 and 33 fishlm3. Water chemistry and water quality characteristics were monitored throughout the 228 day growth trial for all eight systems. A system component performance analys...

  12. A Comparative Analysis of Three Biofilter Types Treating Wastewater Produced in Recirculating Aquaculture Systems

    Hall, Antar Gamble

    1999-01-01

    Nine recirculating systems at the Virginia Tech Aquaculture Center were placed on line and stocked with yellow perch, Perca flavescens, fingerlings. Fish were stocked at a density of approximately 455 fish m-3. Biofilter types were the only factor differing among system designs and were an upflow pulsed bed bead filter, packed tower trickling filter and a rotating biological contactor (RBC). After stocking, systems were allowed to acclimate using ammonia excreted by the yellow perch. Foll...

  13. A COMPARATIVE ANALYSIS OF THREE BIOFILTER TYPES TREATING WASTEWATER PRODUCED IN RECIRCULATING AQUACULTURE SYSTEMS

    Hall, Antar Gamble

    2000-01-01

    Nine recirculating systems at the Virginia Tech Aquaculture Center were placed on line and stocked with yellow perch, Perca flavescens, fingerlings. Fish were stocked at a density of approximately 455 fish m-3. Biofilter types were the only factor differing among system designs and were an upflow pulsed bed bead filter, packed tower trickling filter and a rotating biological contactor (RBC). After stocking, systems were allowed to acclimate using ammonia excreted by the yellow perch. Foll...

  14. A ZigBee Wireless Sensor Network for Monitoring an Aquaculture Recirculating System

    Francisco J. Espinosa-Faller; Guillermo E. Rendón-Rodríguez

    2012-01-01

    A ZigBee wireless sensor network was developed for monitoring an experimental aquaculture recirculating system.Temperature, dissolved oxygen, water and air pressure as well as electric current sensors were included in the setup.The high fish densities required in these systems to become economically viable present a case where sensornetworks can be applied to preserve a healthy livestock and to reduce the risk of failures that end up in the loss ofproduction. Modules for reading and transmitt...

  15. Probiotic effects on cobia Rachycentron canadum larvae reared in a recirculating aquaculture system

    M. Angélica Garrido-Pereira; Michael Schwarz; Brendan Delbos; Ricardo V. Rodrigues; Luis Romano; Luís Sampaio

    2014-01-01

    Cobia (Rachycentron canadum) is a marine finfish with good potential for mariculture. This study analyzes the effects of probiotic Bacillus spp. on the performance of cobia larvae reared in a recirculating aquaculture system (RAS). Larvae were stocked into two independent RAS for 26 days after hatching. One of the systems (Probiotic treatment) received the addition of a commercial probiotic consisting of B. subtilis, B. licheniformis and B. pumilus directly into the water and by live feed. Su...

  16. Water quality in recirculating aquaculture systems (ras) for arctic charr (salvelinus alpinus L.) culture.

    Isla Molleda , M.

    2008-01-01

    Recirculating aquaculture systems (RAS) for fish culture have been used for more than three decades. The interest in RAS is due to their have advantages such as greatly reduced land and water requirements in places where the water resources are limited; but the RAS have also disadvantages like the deterioration of the water quality if the water treatment processes within the system are not controlled properly. The water quality problems in RAS were associated with low DO and high fish waste m...

  17. A ZigBee Wireless Sensor Network for Monitoring an Aquaculture Recirculating System

    Francisco J. Espinosa-Faller

    2012-06-01

    Full Text Available A ZigBee wireless sensor network was developed for monitoring an experimental aquaculture recirculating system.Temperature, dissolved oxygen, water and air pressure as well as electric current sensors were included in the setup.The high fish densities required in these systems to become economically viable present a case where sensornetworks can be applied to preserve a healthy livestock and to reduce the risk of failures that end up in the loss ofproduction. Modules for reading and transmitting sensor values through a ZigBee wireless network were developedand tested. The modules were installed in an aquaculture recirculating system to transmit sensor values to thenetwork coordinator. A monitoring program was created in order to display and store sensor values and to comparethem with reference limits. An alert is emitted in case reference limits have been reached. E-mail and an SMSmessage alert can also be sent to the cellular phone of the system administrator, so immediate action can be taken. Aweb interface allows Internet access to the sensor values. The present work demonstrates the applicability of ZigBeewireless sensor network technology to aquaculture recirculating systems.

  18. Low-dose hydrogen peroxide application in closed recirculating aquaculture systems

    Pedersen, Lars-Flemming; Good, C.; Pedersen, Per Bovbjerg

    2012-01-01

    The aim of the present work was to simulate water treatment practices with hydrogen peroxide (HP) in recirculating aquaculture systems (RAS). Six identical 1,700-L pilot-scale RAS were divided into two experimental groups based on daily feed allocation and operated under constant conditions for a...... for RAS and contradict prevailing notions that HP cannot be used safely in RAS that employ biofiltration. The development of effective new HP treatment protocols for recirculating aquaculture could reduce the current dependence on formalin to improve water quality and control parasitic loads...... period of 3 months. The organic and nitrogenous loadings of the systems differed fourfold between the two groups and were achieved by predefined constant daily feed loads and constant additions of water. The fixed cumulative feed burden was 1.6 × 103 mg feed/L in the low-intensity RAS and 6.3 × 103 mg...

  19. Observations on Side-Swimming Rainbow Trout in Water Recirculation Aquaculture Systems

    Good, Christopher; Davidson, John; Kinman, Christin; Kenney, P. Brett; Bæverfjord, Grete; Summerfelt, Steven

    2014-01-01

    Abstract During a controlled 6-month study using six replicated water recirculation aquaculture systems (WRASs), it was observed that Rainbow Trout Oncorhynchus mykiss in all WRASs exhibited a higher-than-normal prevalence of side swimming (i.e., controlled, forward swimming but with misaligned orientation such that the fish's sagittal axis is approximately parallel to the horizontal plane). To further our understanding of this abnormality, a substudy was conducted wherein side swimmers and n...

  20. Integration of aquaculture and agriculture: a route to sustainable farming systems

    Lightfoot, C. (eds.)

    1990-01-01

    The integration of agriculture and aquaculture as a means of intensifying resource use and improving the productivity of many current farming practices in Southeast Asian and African countries is discussed. A brief account is given of work undertaken by ICLARM in Malawi and India regarding the improved use of marginal lands to integrate crops, vegetables, trees, livestock and fish, outlining also the various problems involved in the extension of such integrated fish farming systems.

  1. Denitrification of Recirculating Aquaculture System Waters Using an Upflow Biofilter and a Fermented Substrate

    Phillips, Jennifer Brooke

    1997-01-01

    The ability of an upflow, denitrifying biofilter using a fermentation generated carbon source to treat the high nitrate concentrations typically seen in recirculating aquaculture systems was studied using a synthetic nitrate wastewater supplied at two nitrate loadings, 1.13 and 2.52 kg NO3-N/m3/day. A supplemental carbon source was provided primarily through the fermentation of fish food which generated volatile fatty acids (VFA) in the form of acetic, propionic, isobutyric, n-butyric, 2-me...

  2. Phylogenetic characterization of the heterotrophic bacterial communities inhabiting a marine recirculating aquaculture system

    Michaud, L; Lo Giudice, A; Troussellier, Marc; Smedile, F; Bruni, V.; Blancheton, J. P.

    2009-01-01

    Aims: The aim of the present work was to characterize the heterotrophic bacterial community of a marine recirculating aquaculture system (RAS). Methods and Results: An experimental RAS was sampled for the rearing water (RW) and inside the biofilter. Samples were analysed for bacterial abundances, community structure and composition by using a combination of culture-dependent and -independent techniques. The most represented species detected among biofilter clones was Pseudomonas stutzeri, whi...

  3. Assessement of Growth Performances of Juvenile Sterlet Acipenser ruthenus Raised in a Recirculating Aquaculture System (RAS)

    Mărioara Nicula; Zoltan-Tibor Szelei; Nicolae Pacala; Marian Bura; Eliza Simiz; Silvia Erina; Marian Hotea; Adela Marcu

    2015-01-01

    The study was conducted within the recirculating aquaculture system for sturgeon rearing in Herneacova village, Timis County, on a population of juvenile sterlet (Acipenser ruthenus) aged between 346-431 days. Specimens were housed in five round tanks (Ø=3 m, V=6 m3, h=100 cm) and fed with commercial extruded pellets (3 mm granulation, 48% CP, and 10% EE). Fish from each tank were individually weighed and measured once every two weeks. Bioproductive indices such body weight gain (BW), specifi...

  4. The technical development and application of a recirculating aquaculture respirometer system (RARS) for fish metabolism studies

    Stiller, Kevin Torben

    2016-01-01

    In dieser Arbeit wurde ein Recirculating Aquaculture Respirometre System (RARS) technisch entwickelt und die Einsatzmöglichkeiten in verschiedenen metabolischen Studien an Fischen unter Einbeziehung der Futtermittelverwertung evaluiert. Kapitel 1 stellt das RARS und die eingebauten online Messgeräte vor. Die Funktionalität des RARS wurde durch einige Beispielmessungen an Regenbogenforellen (Oncorhynchus mykiss) und Steinbutt, (Scophthalmus maximus) gezeigt. Im Kapitel 2 wurden über 8 W...

  5. An economic analysis of the use of recirculating aquaculture systems in the production of Tilapia

    Appiah-Kubi, Frank

    2012-01-01

    An economic analysis of tilapia production was conducted using a recirculating aquaculture system facility, situated at the Norwegian University of Life Science (UMB). The goals were; (1) to evaluate and estimate the operational cost involved and from this, estimate the breakeven cost, (2) identify and describe the constraints unique to the RAS, (3) to perform financial feasibility of a (hypothetical) scale-up production, and (4) to conduct sensitivity analysis on some variables to highlight ...

  6. Conditioning of broodstock of tiger grouper, Epinephelus fuscoguttatus, in a recirculating aquaculture system

    Saleem Mustafa; Mohd. Hafizzie Hajini; Shigeharu Senoo; Annita Yong Seok Kian

    2015-01-01

    Closing the cycle of commercial species of fish in a recirculating aquaculture system is gaining importance for a number of practical advantages. Founder broodstock originating from the wild population is conditioned to live in hatchery tanks under suitable environmental and feeding conditions and is induced to breed. The juveniles are grown to maturity and facilitated to spawn in captivity to close the life cycle in the hatchery. This experiment was carried out on tiger grouper (Epinephelus ...

  7. In situ estimation of water quality parameters in freshwater aquaculture ponds using hyperspectral imaging system

    Abd-Elrahman, Amr; Croxton, Matthew; Pande-Chettri, Roshan; Toor, Gurpal S.; Smith, Scot; Hill, Jeffrey

    Knowledge of water quality parameters is integral to sustainability of freshwater aquaculture operations that raise ornamental fish. Our objective in this study is to evaluate the ability of a mobile, ground-based hyperspectral (HS) imaging sensor to determine chlorophyll-a (Chl-a) concentrations in working aquaculture ponds, which represent manipulated, shallow, nutrient-rich systems, and to determine the effect of using submerged reflectance targets on the accuracy of Chl-a estimation. We collected Chl-a measurements from aquaculture ponds ranging from 0.8 to 494 μg/L. Chl-a measurements showed a strong correlation with two-band and three-band spectral indices computed from the HS image reflectance. Coefficient of determination ( R2) values of 0.975 and 0.982 were obtained for the two- and three-band models, respectively, using spectra captured from the submerged target at 10 cm depth. Using spectra captured from water (no submerged targets), R2 values were slightly lower at 0.833 and 0.862 for two- and three-band models. Data from the submerged target at 30 cm depth had the lowest correlation with measured chlorophyll-a concentrations, potentially due to variations in water column properties and shadows cast by the platform. Modeling total Phosphorous (P) and Nitrogen (N) concentrations of the collected samples with the spectral indices sensitive to Chl-a concentrations showed a moderate level of correlation. Removing a model outlier (observation with maximum N and P concentrations) led to a significant increase in the models' coefficient of determination ( e.g. from 0.478 to 0.823 for the P model using three-band index values), which highlighted the possibility of using HS imagery to estimate N and P concentrations and the need for more research to model the interrelationships between Chl-a and nutrient concentrations in aquaculture water systems.

  8. Aquaculture and rural livelihoods in the Bolivian Amazon - Systems of Innovation and pro-poor technology development

    Canal Beeby, Elisa

    2012-01-01

    This thesis is about pro-poor agricultural innovations and smallholder development in Amazonia. The focus is on aquaculture in the Bolivian Amazon, with particular reference to indigenous territories. An Innovation Systems framework is used to analyse aquaculture Research and Development at a national level and its relevance to small farmers. The analysis of poverty-focused technology development at the project and farm levels is aided by a Knowledge Engineering Approach for agricultural rese...

  9. Bioflocs technology: an integrated system for the removal of nutrients and simultaneous production of feed in aquaculture

    Crab, Roselien

    2010-01-01

    Future development of intensive aquaculture must deal with its impacts on the environment in the form of water pollution and the use of fish oil and fish meal. The bioflocs technology simultaneously addresses both problems co-occurring with the further expansion of the industry. While maintaining good water quality within the aquaculture systems it produces additional feed for the cultured animals. In contrast to conventional water quality control techniques, the bioflocs technology offers a ...

  10. The OMEGA system for marine bioenergy, wastewater treatment, environmental enhancement, and aquaculture

    Trent, J. D.

    2013-12-01

    OMEGA is an acronym for Offshore Membrane Enclosure for Growing Algae. The OMEGA system consists of photobioreactors (PBRs) made of flexible, inexpensive clear plastic tubes attached to floating docks, anchored offshore in naturally or artificially protected bays [1]. The system uses domestic wastewater and CO2 from coastal facilities to provide water, nutrients, and carbon for algae cultivation [2]. The surrounding seawater maintains the temperature inside the PBRs and prevents the cultivated (freshwater) algae from becoming invasive species in the marine environment (i.e., if a PBR module accidentally leaks, the freshwater algae that grow in wastewater cannot survive in the marine environment). The salt gradient between seawater and wastewater is used for forward osmosis (FO) to concentrate nutrients and facilitate algae harvesting [3]. Both the algae and FO clean the wastewater, removing nutrients as well as pharmaceuticals and personal-care products [4]. The offshore infrastructure provides a large surface area for solar-photovoltaic arrays and access to offshore wind or wave generators. The infrastructure can also support shellfish, finfish, or seaweed aquaculture. The economics of the OMEGA system are supported by a combination of biofuels production, wastewater treatment, alternative energy generation, and aquaculture. By using wastewater and operating offshore from coastal cities, OMEGA can be located close to wastewater and CO2 sources and it can avoid competing with agriculture for water, fertilizer, and land [5]. By combining biofuels production with wastewater treatment and aquaculture, the OMEGA system provides both products and services, which increase its economic feasibility. While the offshore location has engineering challenges and concerns about the impact and control of biofouling [6], large OMEGA structure will be floating marine habitats and will create protected 'no-fishing' zones that could increase local biodiversity and fishery

  11. Use of Barley for the Purification of Aquaculture Wastewater in a Hydroponics System

    A. M. Snow

    2008-01-01

    Full Text Available Barley was examined for its ability to remove nutrients from aquaculture wastewater. The effects of seed sterilization using ethanol and bleach and seed density on germination and plant growth were investigated. Surface sterilization of barley seeds had a negative impact on germination. Increasing the ethanol concentration and/or the bleach concentration reduced the germination percentage. Barley seeds were first germinated in water in the hydroponics system. The seedlings then received wastewater from an aquaculture system stocked with Arctic charr. During the experiment, the crops grew rapidly and fairly uniformly and showed no signs of mineral deficiency or disease. The average crop height at harvest was 25.5 cm and the yield varied from 25 to 59 t haˉ1, depending on the seed density. The hydroponically grown barley was able to significantly reduce the pollution load of the aquaculture wastewater. The TS, COD, NH4+-N, NO2--N, NO3--N, and PO43--P reductions ranged from 52.7 to 60.5%, from 72.9 to 83.1%, from 76.0 to 76.0%, from 97.6 to 99.2%, from 76.9 to 81.6% and from 87.1 to 95.1%, respectively. However, the effluent produced from the hydroponics system had slightly higher levels of TS (420-485 mg Lˉ1 than the 480 mg Lˉ1 recommended for aquatic animals. A sedimentation/filtration unit should be added to the hydroponics system.

  12. Particle size distribution in the tilapia Recirculating Aquaculture System

    Stokic, Jelena

    2012-01-01

    This study was to evaluate methods for measuring and describing particle size distribution from three different spots in Tilapia recirculating system at University of Life Ccience in Ås, Norway. For this purpose serial filtration over different mesh size and parallel filtration over different mesh size methods were compared. Water samples were taken from before drum filter, after drum filter and after bio-filter (MBBR) and filtrated through eight different mesh size classes and calculated in ...

  13. Mathematical modelling of nutrient balance of a goldfish (Carassius auratus Linn.) recirculating aquaculture system (GRAS)

    Sudeep Puthravilakom Sadasivan Nair; Sanjib Moulick; Chanchal Kumar Mukherjee; Mohd Tanveer

    2010-01-01

    In the present study, a goldfish (Carassius auratus Linn.) recirculating aquaculture system (GRAS) has been developed. The GRAS consisted of a culture tank, a screen filter and a foam fractionator for removal of particulate and dissolved solids and a trickling filter for conversion of ammonium- and nitrite-nitrogen to relatively harmless nitrate-nitrogen. The culture of goldfish at a stocking density of 1.08 kg/m3 was continued for a period of two and half months. Based on mass balance analys...

  14. The influence of pellets quality on the growth of sterlet, in recirculating aquaculture system

    Corina Sion; Petronela G. Călin; Lucian Oprea; Aurelia Nica; Gianina M. Băcanu

    2011-01-01

    The paper presents some aspects regarding the influence of food quality on the breeding of sterlet, Acipenser ruthenus Linnaeus, 1758, in a recirculating aquaculture system (RAS). The experiment took place over 30 days, in four pilot breeding units type aquaria of 300 liters in volume. Two kind of variants were compared, with repetition, V1 with 46% crude protein and V2 with 30% crude protein, respectively. The stocking density was of 14 fish/unit. The same feeding level of 8 g/kg metabolic w...

  15. Removal efficiency and balance of nitrogen in a recirculating aquaculture system integrated with constructed wetlands.

    Zhong, Fei; Liang, Wei; Yu, Tao; Cheng, Shui P; He, Feng; Wu, Zhen B

    2011-01-01

    The nitrogen (N) balance for aquaculture is an important aspect, especially in China, and it is attributed to the eutrophication in many freshwater bodies. In recent years, constructed wetlands (CWs) have been widely used in wastewater treatment and ecosystem restoration. A recirculating aquaculture system (RAS) consisting of CWs and 4 fish ponds was set up in Wuhan, China. Channel catfish (Ictalurus punctatus) fingerlings were fed for satiation daily for 168 days with 2 diets containing 5.49 % and 6.53 % nitrogen, respectively. The objectives of this study were to investigate the N budget in the RAS, and try to find out the feasibility of controlling N accumulation in the fish pond. It is expected that the study can provide a mass balance for the fate of N in the eco-friendly treatment system to avoid eutrophication. The results showed that the removal rates of ammonia (NH(+)(4)-N), sum of nitrate & nitrite (NO(-)(X)-N), and total nitrogen (TN) by the CWs were 20-55%, 38-84 % and 39-57 %, respectively. Denitrification in the CWs was the main pathway of nitrogen loss (41.67 %). Nitrogen accumulation in pond water and sediment accounted for 3.39 % and 12.65 % of total nitrogen loss, respectively. The nitrogen removal efficiency and budget showed that the CW could be used to control excessive nitrogen accumulation in fish ponds. From the viewpoint of the nitrogen pollution control, the RAS combined with the constructed wetland can be applied to ensure the sustainable development for aquaculture. PMID:21644158

  16. Program to monitor and evaluate a passive solar greenhouse/aquaculture system. Final report

    1982-01-01

    A temperature monitoring program of Amity's solar greenhouse demonstrated that air, soil, and water temperatures can be maintained at optimal levels without supplemental heat. A foil reflector placed in front of the greenhouse glazing at an angle of between 0 and 5/sup 0/ above horizontal enhanced direct light entering the greenhouse by as much as 22%. Aquaculture in the water heat storage of a solar greenhouse has been a success. Fish reached harvest size in about seven months. The two species that were received the best by the public were African perch (Tilapia mossambica) and channel catfish (Ictalurus punctatus). Although carp (Cyprinus carpio) were the fastest growers they were not well received by the public. Linking hydroponics to greenhouse aquaculture shows a lot of promise. Different support medias were examined and tomatoes and European cucumbers were raised successfully. A savonius windmill was successfully linked to an aquaculture aeration system but because of the wind pattern in the Willamette valley the windmill system did not provide air in the evening when it was needed most. Alternate designs are discussed. Locally grown fish diets were evaluated for their ability to promote fish growth. Diets such as water hyacinth, duckweed, earthworms, beans, and comfrey were raised on the Amity site, pelleted with a hand grinder and solar dried. Duckweed and earthworms appear to hold promise for a nutritous, easy to grow and pelletize, food source. Amity's solar greenhouse, three coldframe designs and a PVC tunnel cloche were compared in a vegetable growing trial. Most impressive was the cloche design because it provided adequate protection, was inexpensive and very easy to build.

  17. Nitrification in brackish water recirculating aquaculture system integrated with activated packed bed bioreactor.

    Rejish Kumar, V J; Joseph, Valsamma; Philip, Rosamma; Bright Singh, I S

    2010-01-01

    Recirculation aquaculture systems (RAS) depend on nitrifying biofilters for the maintenance of water quality, increased biosecurity and environmental sustainability. To satisfy these requirements a packed bed bioreactor (PBBR) activated with indigenous nitrifying bacterial consortia has been developed and commercialized for operation under different salinities for instant nitrification in shrimp and prawn hatchery systems. In the present study the nitrification efficiency of the bioreactor was tested in a laboratory level recirculating aquaculture system for the rearing of Penaeus monodon for a period of two months under higher feeding rates and no water exchange. Rapid setting up of nitrification was observed during the operation, as the volumetric total ammonia nitrogen removal rates (VTR) increased with total ammonia nitrogen (TAN) production in the system. The average Volumetric TAN Removal Rates (VTR) at the feeding rate of 160 g/day from 54-60th days of culture was 0.1533+/-0.0045 kg TAN/m(3)/day. The regression between VTR and TAN explained 86% variability in VTR (P<0.001). The laboratory level RAS demonstrated here showed high performance both in terms of shrimp biomass yield and nitrification and environmental quality maintenance. Fluorescent in-situ Hybridization analysis of the reactor biofilm ensured the presence of autotrophic nitrifier groups such as Nitrosococcus mobilis lineage, Nitrobacter spp and phylum Nitrospira, the constituent members present in the original consortia used for activating the reactors. This showed the stability of the consortia on long term operation. PMID:20150717

  18. Characterising organic matter in recirculating aquaculture systems with fluorescence EEM spectroscopy.

    Hambly, A C; Arvin, E; Pedersen, L-F; Pedersen, P B; Seredyńska-Sobecka, B; Stedmon, C A

    2015-10-15

    The potential of recirculating aquaculture systems (RAS) in the aquaculture industry is increasingly being acknowledged. Along with intensified application, the need to better characterise and understand the accumulated dissolved organic matter (DOM) within these systems increases. Mature RASs, stocked with rainbow trout and operated at steady state at four feed loadings, were analysed by dissolved organic carbon (DOC) analysis and fluorescence excitation-emission matrix (EEM) spectroscopy. The fluorescence dataset was then decomposed by PARAFAC analysis using the drEEM toolbox. This revealed that the fluorescence character of the RAS water could be represented by five components, of which four have previously been identified in fresh water, coastal marine water, wetlands and drinking water. The fluorescence components as well as the DOC showed positive correlations with feed loading, however there was considerable variation between the five fluorescence components with respect to the degree of accumulation with feed loading. The five components were found to originate from three sources: the feed; the influent tap water (groundwater); and processes related to the fish and the water treatment system. This paper details the first application of fluorescence EEM spectroscopy to assess DOM in RAS, and highlights the potential applications of this technique within future RAS management strategies. PMID:26141427

  19. Assessement of Growth Performances of Juvenile Sterlet Acipenser ruthenus Raised in a Recirculating Aquaculture System (RAS

    Mărioara Nicula

    2015-10-01

    Full Text Available The study was conducted within the recirculating aquaculture system for sturgeon rearing in Herneacova village, Timis County, on a population of juvenile sterlet (Acipenser ruthenus aged between 346-431 days. Specimens were housed in five round tanks (Ø=3 m, V=6 m3, h=100 cm and fed with commercial extruded pellets (3 mm granulation, 48% CP, and 10% EE. Fish from each tank were individually weighed and measured once every two weeks. Bioproductive indices such body weight gain (BW, specific growth rate (SGR, and feed conversion ratio (FCR were calculated at the end of an 85 days experimental period. Statistical analysis was performed using the SPPS IBM 22 and Minitab 16 software. Data were reported as Mean±Sx at a significant level of p<0.05. Testing differences between means was realized by ANOVA completed with post-hoc Tukey test. The results obtained indicate that the growth performance of juvenile Acipenser ruthenus seems to be similar and even better than that of other sturgeons species reared in recirculating aquaculture system.

  20. Determination of oxytetracycline residues in matrixes from a freshwater recirculating aquaculture system.

    Carson, Mary C; Bullock, Graham; Bebak-Williams, Julie

    2002-01-01

    This paper describes related procedures to determine the amount of oxytetracycline (OTC) present in trout tissue (muscle with skin attached), biofilter sand, sediment, and tank water from a recirculating aquaculture system. OTC was extracted from the matrixes by different techniques, depending on complexity of the matrix and desired OTC detection level in that matrix. Listed in order of increasing complexity, OTC was extracted from tank water by dilution with acidic buffer containing ethylenediaminetetraacetic acid (EDTA); from biofilter sand by shaking with 0.1 N HCl; from sediment by homogenization and shaking with buffer/EDTA; and from ground trout by homogenization and shaking with buffer/EDTA (twice), with further cleanup and concentration of the extract on a polymeric solid-phase extraction cartridge. The 4 procedures all used the same reversed-phase gradient chromatography on a polymeric column with UV detection at 350 nm. The lower limit of detection (estimated) and upper limit of validation for each of these 4 matrixes were 0.04-4.0 microg/g (ppm; trout), 0.03-20 ppm (biofilter sand), 1-6000 ppm (sediment), and 0.003-10 ppm (water). Recoveries ranged from 82 to 108%, with relative standard deviation recirculating aquaculture system. PMID:11990017

  1. Daily micro particle distribution of an experimental recirculating aquaculture system – A case study

    Fernandes, Paulo; Pedersen, Lars-Flemming; Pedersen, Per Bovbjerg

    2014-01-01

    The particle size distribution (PSD) in a recirculating aquaculture system (RAS) was investigated duringa 24-h cycle. PSD was analyzed in water sampled at several locations in a recirculation loop containing a60-m drum filter, a submerged fixed-bed biofilter and a trickling filter.In relation to...... total counts, the system was dominated by micro-particles with particles smaller than20 m comprising >94% of the distribution in all samples. However, the system presented a substantialvolumetric influence of larger particles, reflected by a PSD derivate ˇ-value of 3.40 ± 0.18. Overall ˇ......-valuesthroughout the compartments (p = 0.584) and experimental period (p = 0.217) were not significantlydifferent, although specific components seemed to marginally affect the PSD.A high internal water turnover rate (one system passage every 50 min) promoted the rapid removalof large particles from the system...

  2. Environmental quality and aquaculture systems: Proceedings of the Thirteenth U.S.-Japan Meeting on Aquaculture, Mie, Japan, October 24-25, 1984

    1988-01-01

    The United States and Japanese counterpart panels on aquaculture were formed in 1969 under the United States-Japan Cooperative Program in Natural Resources (UJNR). The panels currently include specialists drawn from the federal departments most concerned with aquaculture. Charged with exploring and developing bilateral cooperation, the panels have focused their efforts on exchanging information related to aquaculture which could be of benefit to both countries. The UJNR was begun during t...

  3. Indicative and pathogenic microbiological quality of aquacultured finfish grown in different production systems.

    Pullela, S; Fernandes, C F; Flick, G J; Libey, G S; Smith, S A; Coale, C W

    1998-02-01

    The nature and number of indicator and pathogenic microbes in fish reared using recirculating and nonrecirculating water systems were compared. For each system, 20 samples of rainbow trout (Oncorhynchus mykiss), tilapia (Oreochromis spp.), hybrid striped bass (Morone saxatilis x M. chrysops), and pacu (Piaractus mesopotamicus) were randomly selected and gutted, and microbial analyses were performed using AOAC procedures. Five fish were subsampled and analyzed for indicative microbial quality with 3M Petrifilm (). The general microbial quality differed significantly (P systems, except for total coliform counts. Rainbow trout cultured in recirculating and nonrecirculating water systems had lower counts for aerobes (2.00 to 3.11 log CFU/g) (p recirculating water system had significantly lower psychrotrophic numbers (0.86 to 1.85 log CFU/g). Pacu had the highest fecal coliform counts (2.74 to 3.70 log CFU/g), whereas hybrid striped bass and rainbow trout grown in nonrecirculating systems had lower fecal coliform counts (0.00 to 1.39 log CFU/g). Rainbow trout grown in a nonrecirculating system had significantly higher Escherichia coli counts (0.00 to 2.11 log CFU/g). The human bacterial pathogens Listeria monocytogenes, Yersinia enterocolitica, Escherichia coli O157:H7 and Salmonella spp. were not isolated form the fish sampled. However, Clostridium botulinum botulinum was isolated from all the aquacultured fish sampled except pacu and tilapia grown in a recirculating aquaculture system. However, the counts were very low, ranging from 0.0 to 2.3 MPN/g. PMID:9708283

  4. Integrated fish-seaweed cultivation in recirculating aquaculture systems: objectives and preliminary results of "IntegRAS" project

    Oca Baradad, Joan; Pintado Valverde, José; Cremades Ugarte, Javier; Masaló Llorà, Ingrid; Ruiz, Patricia; Alamrousi, A; Jiménez de Ridder, Patrícia; Machado, Samuel; Reig Puig, Lourdes

    2015-01-01

    Recirculating aquaculture systems (RAS) allow minimizing the water renewal flow rate needed in fish production by increased Nitrogen (NO3 ) and Phosphorous concentrations. Integrating seaweed production to RAS can improve its performances and reduce soluble wastes, contributing to a more sustainable production system.

  5. Probiotic effects on cobia Rachycentron canadum larvae reared in a recirculating aquaculture system

    M. Angélica Garrido-Pereira

    2014-11-01

    Full Text Available Cobia (Rachycentron canadum is a marine finfish with good potential for mariculture. This study analyzes the effects of probiotic Bacillus spp. on the performance of cobia larvae reared in a recirculating aquaculture system (RAS. Larvae were stocked into two independent RAS for 26 days after hatching. One of the systems (Probiotic treatment received the addition of a commercial probiotic consisting of B. subtilis, B. licheniformis and B. pumilus directly into the water and by live feed. Survival, final weight and water quality were not affected by probiotics. Results showed larvae of the probiotic treatment demonstrated a greater resistance to salinity stress. Immunohistochemical analysis showed a higher expression of CD4 in probiotic treatment. These results suggest that Bacillus spp. probiotics used in RAS have a potential stimulating impact on immune system differentiation and increases salinity stress resistance of cobia larvae.

  6. Recirculating aquaculture system for high density production of the calanoid copepod Acartia tonsa (Dana)

    Vu, Minh Thi Thuy; Øie, Gunvor; Reinertsen, Helge

    . Both RAS and FTAS (3 replicates per system) were set up in the same room to ensure the equal condition. The initial densities of copepods were 20000 nauplii L-1 for investigation of growth and development in the early phase and 5000 ind L-1 in the copepodite and adult stages for testing reproduction...... similar survival, growth, and reproduction, yet the nauplii developed into copepodites faster in RAS (110h) compared to FTAS (158h). This can be an indication for the potential for culturing or maintaining A. tonsa nauplii and early copepodite stages at higher densities before feeding larvae of marine...... fish larvae. Yet, the biomass and egg production of A. tonsa has been limited mainly due to the challanges to culture them at high density. The development of recirculating aquaculture system (RAS) in recent decades has opened a new culturing system that is expected to provide more stable environmental...

  7. The influence of different stocking densities on biochemical composition of rainbow trout meat reared in a recirculating aquaculture system

    Mirela Cretu; Cristea Victor; Dediu Lorena; Petrea Stefan Mihai

    2014-01-01

    The aim of this study is to evaluate the biochemical composition of rainbow trout meat, reared in a recirculating aquaculture system, at different stocking densities (DS1-2,64 kg/m3, DS2-5,16 kg/m3, DS3-7,12 kg/m3, DS4-9,42 kg/m3). The analyses were performed in the research laboratory of Aquaculture, Environmental Science and Cadastre Department – Faculty of Food Science and Engineering, from ,,Dunarea de Jos’’University of Galati. The content of meat proteins, fats, dry substance and ash wa...

  8. The potential use of constructed wetlands in a recirculating aquaculture system for shrimp culture

    Lin, Y.-F.; Jing, S.-R.; Lee, D.-Y

    2003-05-01

    Constructed wetlands improved water qualities and consequently increased the shrimp growth and survival in a recirculating system. - A pilot-scale constructed wetland unit, consisting of free water surface (FWS) and subsurface flow (SF) constructed wetlands arranged in series, was integrated into an outdoor recirculating aquaculture system (RAS) for culturing Pacific white shrimp (Litopenaeus vannamei). This study evaluated the performance of the wetland unit in treating the recirculating wastewater and examined the effect of improvement in water quality of the culture tank on the growth and survival of shrimp postlarvae. During an 80-day culture period, the wetland unit operated at a mean hydraulic loading rate of 0.3 m/day and effectively reduced the influent concentrations of 5-day biochemical oxygen demand (BOD{sub 5}, 24%), suspended solids (SS, 71%), chlorophyll a (chl-a, 88%), total ammonium (TAN, 57%), nitrite nitrogen (NO{sub 2}-N, 90%) and nitrate nitrogen (NO{sub 3}-N, 68%). Phosphate (PO{sub 4}-P) reduction was the least efficient (5.4%). The concentrations of SS, Chl-a, turbidity and NO{sub 3}-N in the culture tank water in RAS were significantly (P{<=}0.05) lower than those in a control aquaculture system (CAS) that simulated static pond culture without wetland treatment. However, no significant difference (P{<=}0.05) in BOD{sub 5}, TAN and NO{sub 2}-N was found between the two systems. At the end of the study, the harvest results showed that shrimp weight and survival rate in the RAS (3.8{+-}1.8 g/shrimp and 90%) significantly (P{<=}0.01) exceeded those in the CAS (2.3{+-}1.5 g/shrimp and 71%). This study concludes that constructed wetlands can improve the water quality and provide a good culture environment, consequently increasing the shrimp growth and survival without water exchange, in a recirculating system.

  9. The potential use of constructed wetlands in a recirculating aquaculture system for shrimp culture

    Constructed wetlands improved water qualities and consequently increased the shrimp growth and survival in a recirculating system. - A pilot-scale constructed wetland unit, consisting of free water surface (FWS) and subsurface flow (SF) constructed wetlands arranged in series, was integrated into an outdoor recirculating aquaculture system (RAS) for culturing Pacific white shrimp (Litopenaeus vannamei). This study evaluated the performance of the wetland unit in treating the recirculating wastewater and examined the effect of improvement in water quality of the culture tank on the growth and survival of shrimp postlarvae. During an 80-day culture period, the wetland unit operated at a mean hydraulic loading rate of 0.3 m/day and effectively reduced the influent concentrations of 5-day biochemical oxygen demand (BOD5, 24%), suspended solids (SS, 71%), chlorophyll a (chl-a, 88%), total ammonium (TAN, 57%), nitrite nitrogen (NO2-N, 90%) and nitrate nitrogen (NO3-N, 68%). Phosphate (PO4-P) reduction was the least efficient (5.4%). The concentrations of SS, Chl-a, turbidity and NO3-N in the culture tank water in RAS were significantly (P≤0.05) lower than those in a control aquaculture system (CAS) that simulated static pond culture without wetland treatment. However, no significant difference (P≤0.05) in BOD5, TAN and NO2-N was found between the two systems. At the end of the study, the harvest results showed that shrimp weight and survival rate in the RAS (3.8±1.8 g/shrimp and 90%) significantly (P≤0.01) exceeded those in the CAS (2.3±1.5 g/shrimp and 71%). This study concludes that constructed wetlands can improve the water quality and provide a good culture environment, consequently increasing the shrimp growth and survival without water exchange, in a recirculating system

  10. Holographic Aquaculture

    Ian, Richard; King, Elisabeth

    1988-01-01

    Proposed is an exploratory study to verify the feasibility of an inexpensive micro-climate control system for both marine and freshwater pond and tank aquaculture, offering good control over water temperature, incident light flux, and bandwidth, combined with good energy efficiency. The proposed control system utilizes some familiar components of passive solar design, together with a new holographic glazing system which is currently being developed by, and proprietary to Advanced Environmental Research Group (AERG). The use of solar algae ponds and tanks to warm and purify water for fish and attached macroscopic marine algae culture is an ancient and effective technique, but limited seasonally and geographically by the availability of sunlight. Holographic Diffracting Structures (HDSs) can be made which passively track, accept and/or reject sunlight from a wide range of altitude and azimuth angles, and redirect and distribute light energy as desired (either directly or indirectly over water surface in an enclosed, insulated structure), effectively increasing insolation values by accepting sunlight which would not otherwise enter the structure.

  11. Electron beam irradiation, oxygen, and temperature effects on nucleotide degradation in stored aquaculture hybrid striped bass fillets

    Skinless fillets from commercially-grown aquaculture hybrid striped bass (Morone saxatilis x M. chrysops) were electron beam-irradiated in the presence of air or vacuum-packaged and stored at 4C and -20C for 14 days. A mean low dose level of 2.0 or 3.0 kGy (+/- 0.5 kGy) and high dose level of 20 kGy (+/- 4 kGy) were used for irradiated samples. Hypoxanthine (Hx) concentrations, Ki-values ([(INO + Hx)/(IMP + INO + Hx)] x 100), and H-values ([(Hx)/(IMP + INO + Hx)] x 100) indicated that irradiation did not influence the rate of nucleotide degradation compared with nonirradiated controls at either refrigerated or frozen temperatures. Vacuum packaging or freezing of stored samples resulted in lower H-values and Hx contents compared with nonirradiated controls regardless of irradiation treatment

  12. A highly sensitive underwater video system for use in turbid aquaculture ponds.

    Hung, Chin-Chang; Tsao, Shih-Chieh; Huang, Kuo-Hao; Jang, Jia-Pu; Chang, Hsu-Kuang; Dobbs, Fred C

    2016-01-01

    The turbid, low-light waters characteristic of aquaculture ponds have made it difficult or impossible for previous video cameras to provide clear imagery of the ponds' benthic habitat. We developed a highly sensitive, underwater video system (UVS) for this particular application and tested it in shrimp ponds having turbidities typical of those in southern Taiwan. The system's high-quality video stream and images, together with its camera capacity (up to nine cameras), permit in situ observations of shrimp feeding behavior, shrimp size and internal anatomy, and organic matter residues on pond sediments. The UVS can operate continuously and be focused remotely, a convenience to shrimp farmers. The observations possible with the UVS provide aquaculturists with information critical to provision of feed with minimal waste; determining whether the accumulation of organic-matter residues dictates exchange of pond water; and management decisions concerning shrimp health. PMID:27554201

  13. Degradation and effect of hydrogen peroxide in small-scale recirculation aquaculture system biofilters

    Møller, Martin Sune; Arvin, Erik; Pedersen, Lars-Flemming

    2010-01-01

    applied in different systems. In this study, we investigated the degradation kinetics of HP in biofilters from water recirculating aquaculture systems (RAS). The potential effect of HP on the nitrification process in the biofilters was also examined. Biofilter elements from two different pilot-scale RAS......−1 maintained over 3 h had a moderate inhibitory effect on the biofilter elements from one of the RAS with relatively high organic loading, while the nitrification was severely inhibited in the pilot-scale biofilters from the other RAS with a relatively low organic loading. A pilot-scale RAS......, equipped with two biofilter units, both a moving-bed (Biomedia) and a fixed-bed (BIO-BLOK®) biofilter, was subjected to an average HP concentration of ∼12 mg L−1 for 3 h. The ammonium- and nitrite-degrading efficiencies of both the Biomedia and the BIO-BLOK® filters were drastically reduced. The filters...

  14. Modelling for an improved integrated multi-trophic aquaculture system for the production of highly valued marine species

    Luana Granada

    2014-05-01

    Full Text Available Integrated multi-trophic aquaculture (IMTA is regarded as a suitable approach to limit aquaculture nutrients and organic matter outputs through biomitigation. Here, species from different trophic or nutritional levels are connected through water transfer. The co-cultured species are used as biofilters, and each level has its own independent commercial value, providing both economic and environmental sustainability. In order to better understand and optimize aquaculture production systems, dynamic modelling has been developed towards the use of models for analysis and simulation of aquacultures. Several models available determine the carrying capacity of farms and the environmental effects of bivalve and fish aquaculture. Also, in the last two decades, modelling strategies have been designed in order to predict the dispersion and deposition of organic fish farm waste, usually using the mean settling velocity of faeces and feed pellets. Cultured organisms growth, effects of light and temperature on algae growth, retention of suspended solids, biodegradation of nitrogen and wastewater treatment are examples of other modelled parameters in aquaculture. Most modelling equations have been developed for monocultures, despite the increasing importance of multi-species systems, such as polyculture and IMTA systems. The main reason for the development of multi-species models is to maximize the production and optimize species combinations in order to reduce the environmental impacts of aquaculture. Some multi-species system models are available, including from the polyculture of different species of bivalves with fish to more complex systems with four trophic levels. These can incorporate ecosystem models and use dynamic energy budgets for each trophic group. In the proposed IMTA system, the bioremediation potential of the marine seaweed Gracilaria vermiculophylla (nutrient removal performance and the Mediterranean filter-feeding polychaete Sabella

  15. The impact of water exchange rate on the health and performance of rainbow trout Oncorhynchus mykiss in recirculation aquaculture systems

    Fish mortality in recirculating aquaculture systems (RAS) has been observed by the authors to increase when RAS are managed at low makeup water exchange rates with relatively high feed loading. The precise etiology of this elevated mortality was unknown, all typical water quality parameters were wit...

  16. Abnormal swimming behavior and increased deformities in rainbow trout Oncorhynchus mykiss cultured in low exchange water recirculation aquaculture systems

    Two studies were conducted to determine if accumulating water quality parameters would negatively impact rainbow trout Oncorhynchus mykiss health and welfare within water recirculation aquaculture systems (WRAS) that were operated at low and near-zero water exchange, with and without ozonation, and ...

  17. Production characteristics and body composition of juvenile cobia fed three different commercial diets in recirculating aquaculture systems

    The effect of feeding three commercial diets on production characteristics and body composition of juvenile cobia Rachycentron canadum reared using recirculating aquaculture systems (RAS) was evaluated in a 56 d growth trial. Juvenile cobia (29.2 +/= 0.7 g, mean weight +/= SE) were stocked into thr...

  18. Preliminary studies on the depuration of common off-flavors from fish raised in recirculating aquaculture systems

    “Off-flavor” problems can adversely impact the growth of the aquaculture industry. Fish raised in recirculating systems have the potential to develop the common off-flavors “earthy” and “musty” due to accumulation of the microbial metabolites geosmin and 2-methylisoborneol (MIB) in the fish flesh. ...

  19. The role of a fish pond in optimizing nutrient flows in integrated agriculture-aquaculture farming systems

    Nhan, D.K.

    2007-01-01

      In the Mekong delta, the Vietnamese government promoted integrated agriculture-aquaculture (IAA) farming systems as an example of sustainable agriculture. An important advantage of IAA-farming is the nutrient linkage between the pond and terrestrial components within a f

  20. Comparative performance of CO2 measuring methods: marine aquaculture recirculation system application

    Pfeiffer, T.J.; Summerfelt, S.T.; Watten, B.J.

    2011-01-01

    Many methods are available for the measurement of dissolved carbon dioxide in an aqueous environment. Standard titration is the typical field method for measuring dissolved CO2 in aquaculture systems. However, titrimetric determination of dissolved CO2 in marine water aquaculture systems is unsuitable because of the high dissolved solids, silicates, and other dissolved minerals that interfere with the determination. Other methods used to measure dissolved carbon dioxide in an aquaculture water included use of a wetted CO2 probe analyzer, standard nomographic methods, and calculation by direct measurements of the water's pH, temperature, and alkalinity. The determination of dissolved CO2 in saltwater based on partial pressure measurements and non-dispersive infra-red (NDIR) techniques with a CO2 gas analyzer are widely employed for oceanic surveys of surface ocean CO2 flux and are similar to the techniques employed with the head space unit (HSU) in this study. Dissolved carbon dioxide (DC) determination with the HSU using a infra-red gas analyzer (IRGA) was compared with titrimetric, nomographic, calculated, and probe measurements of CO2 in freshwater and in saltwater with a salinity ranging from 5.0 to 30 ppt, and a CO2 range from 8 to 50 mg/L. Differences in CO2 measurements between duplicate HSUs (0.1–0.2 mg/L) were not statistically significant different. The coefficient of variation for the HSU readings averaged 1.85% which was better than the CO2 probe (4.09%) and that for the titrimetric method (5.84%). In all low, medium and high salinity level trials HSU precision was good, averaging 3.39%. Differences existed between comparison testing of the CO2 probe and HSU measurements with the CO2 probe readings, on average, providing DC estimates that were higher than HSU estimates. Differences between HSU and titration based estimates of DC increased with salinity and reached a maximum at 32.2 ppt. These differences were statistically significant (P < 0.05) at all

  1. Characterization of integrons and tetracycline resistance determinants in Aeromonas spp. isolated from South African aquaculture systems.

    Jacobs, Liezl; Chenia, Hafizah Y

    2007-03-20

    An increasing incidence of multidrug resistance amongst Aeromonas spp. isolates, which are both fish pathogens and emerging opportunistic human pathogens, has been observed worldwide. This can be attributed to the horizontal transfer of mobile genetic elements, viz.: plasmids and class 1 integrons. The antimicrobial susceptibilities of 37 Aeromonas spp. isolates, from tilapia, trout and koi aquaculture systems, were determined by disc-diffusion testing. The plasmid content of each isolate was examined using the alkaline lysis protocol. Tet determinant type was determined by amplification using two degenerate primer sets and subsequent HaeIII restriction. The presence of integrons was determined by PCR amplification of three integrase genes, as well as gene cassettes, and the qacEDelta1-sulI region. Thirty-seven Aeromonas spp. isolates were differentiated into six species by aroA PCR-RFLP, i.e., A. veronii biovar sobria, A. hydrophila, A. encheleia, A. ichtiosoma, A. salmonicida, and A. media. High levels of resistance to tetracycline (78.3%), amoxicillin (89.2%), and augmentin (86.5%) were observed. Decreased susceptibility to erythromycin was observed for 67.6% of isolates. Although 45.9% of isolates displayed nalidixic acid resistance, majority of isolates were susceptible to the fluoroquinolones. The MAR index ranged from 0.12 to 0.59, with majority of isolates indicating high-risk contamination originating from humans or animals where antibiotics are often used. Plasmids were detected in 21 isolates, with 14 of the isolates displaying multiple plasmid profiles. Single and multiple class A family Tet determinants were observed in 27% and 48.7% of isolates, respectively, with Tet A being the most prevalent Tet determinant type. Class 1 integron and related structures were amplified and carried different combinations of the antibiotic resistance gene cassettes ant(3'')Ia, aac(6')Ia, dhfr1, oxa2a and/or pse1. Class 2 integrons were also amplified, but the

  2. Tracing dissolved organic matter (DOM) from land-based aquaculture systems in North Patagonian streams

    Nimptsch, Jorge; Woelfl, Stefan; Osorio, Sebastian;

    2015-01-01

    Chile is the second largest producer of salmonids worldwide. The first step in the production of salmonids takes place in land-based aquacultures. However, the effects of the discharge from these aquacultures on stream dissolved organic matter (DOM) content, molecular composition and degradabilit...

  3. Broodstock management of the fine flounder Paralichthys adspersus (Steindachner, 1867) using recirculating aquaculture systems

    Lili Carrera; Noemí Cota; Melissa Montes; Enrique Mateo; Verónica Sierralta; Teresa Castro; Angel Perea; Cristian Santos; Christian Catcoparco; Carlos Espinoza

    2013-01-01

    The present study describes the methodology used at IMARPE for the capture, acclimation and management of P. adspersus broodstock using recirculating aquaculture systems (RAS). RAS improved the water quality and maintained the environmental parameters during the acclimation period, temperature (17.2±1°C), oxygen (8.1±0.7 mg L-1), pH (7.3±0.2), ammonia (0.004±0.003 mg L-1), nitrite (0.52±0.2 mg L-1) and nitrate (3.45±2.6 mg L-1). Fish began to be fed normally from day 15 post-capture, once or ...

  4. BIOFILTERS IN AQUACULTURE

    Ivo Šarić

    2010-07-01

    Full Text Available Recirculating aquaculture is one of the solutions to an environmentally sustainable and economically feasible aquaculture production, and can be established in either urban or rural communities. Controlled conditions enable optimal growth conditions suitable for the species in question during the whole growth process, as well as the shortening of the process itself, when compared to open uncontrolled systems. Recirculating systems are now one of the most researched fields of aquaculture, and biofiltration as the reduction of the poisonous ammonia compounds generated by digestion of proteins, is one of its most important parts. The aim of this study was to stress the importance of biofiltration, and to describe different designs of biofilters. Many biofilters are being used in commercial and research aquaculture facilities with differences in choice of working organism, design, material, price and etc. For the proper choice of biofilter it is necessary to know characteristics of each recirculating aquaculture system, because there are still no strict criteria for categorizing and applying different biofilter designs.

  5. Numerical simulation of the hydrodynamics within octagonal tanks in recirculating aquaculture systems

    Liu, Yao; Liu, Baoliang; Lei, Jilin; Guan, Changtao; Huang, Bin

    2016-07-01

    A three-dimensional numerical model was established to simulate the hydrodynamics within an octagonal tank of a recirculating aquaculture system. The realizable k-ɛ turbulence model was applied to describe the flow, the discrete phase model (DPM) was applied to generate particle trajectories, and the governing equations are solved using the finite volume method. To validate this model, the numerical results were compared with data obtained from a full-scale physical model. The results show that: (1) the realizable k-ɛ model applied for turbulence modeling describes well the flow pattern in octagonal tanks, giving an average relative error of velocities between simulated and measured values of 18% from contour maps of velocity magnitudes; (2) the DPM was applied to obtain particle trajectories and to simulate the rate of particle removal from the tank. The average relative error of the removal rates between simulated and measured values was 11%. The DPM can be used to assess the self-cleaning capability of an octagonal tank; (3) a comprehensive account of the hydrodynamics within an octagonal tank can be assessed from simulations. The velocity distribution was uniform with an average velocity of 15 cm/s; the velocity reached 0.8 m/s near the inlet pipe, which can result in energy losses and cause wall abrasion; the velocity in tank corners was more than 15 cm/s, which suggests good water mixing, and there was no particle sedimentation. The percentage of particle removal for octagonal tanks was 90% with the exception of a little accumulation of ≤ 5 mm particle in the area between the inlet pipe and the wall. This study demonstrated a consistent numerical model of the hydrodynamics within octagonal tanks that can be further used in their design and optimization as well as promote the wide use of computational fluid dynamics in aquaculture engineering.

  6. Aquaculture marketing

    Surtida, Marilyn B.

    2000-01-01

    Aquaculture production is meaningless unless distributed and consumed. Most of the time, the technology generated addresses production, never mind distribution and consumption. Experts say that if production is to be increased, distribution 'from the point of production to the point of consumption must complement the increase.' As fish production increases, marketing must be efficient to allow it to expand.

  7. Fate of water borne therapeutic agents and associated effects on nitrifying biofilters in recirculating aquaculture systems

    Pedersen, Lars-Flemming

    Recent discharge restrictions on antibiotics and chemotherapeutant residuals used in aquaculture have several implications to the aquaculture industry. Better management practices have to be adopted, and documentation and further knowledge of the chemical fate is required for proper administration...... and to support the ongoing development of a sustainable aquaculture industry. A focal point of this thesis concerns formaldehyde (FA), a commonly used chemical additive with versatile aquaculture applications. FA is safe for use with fish and has a high treatment efficiency against fungal and parasite...... ≥2 mg/L. PAA and HP decay patterns were significantly affected by water quality parameters, i.e. at low organic matter content HP degradation was impeded due to microbial inhibition. FISH analysis on biofilm samples from two different types of RAS showed that Nitrosomonas oligotropha was the dominant...

  8. Evaluation of Internalisation Processes in Environmental Management Systems: Aquaculture and Offshore Mussel Farming

    Malorgio, Giulio

    2005-01-01

    In recent years, particular attention has been paid to fishing activities, and more specifically to aquaculture, a production process whose environmental impact at present is not being adequately submitted to any sort of serious assessment and control. The impact of aquaculture on the environment has nonetheless become an important issue for both potential customers and public opinion. The purpose of this study is to appraise environmental protection costs if and when a certified program of e...

  9. Biomass production and nitrogen dynamics in an integrated aquaculture/agriculture system

    Owens, L. P.; Hall, C. R.

    1990-01-01

    A combined aquaculture/agriculture system that brings together the three major components of a Controlled Ecological Life Support System (CELSS) - biomass production, biomass processing, and waste recycling - was developed to evaluate ecological processes and hardware requirements necessary to assess the feasibility of and define design criteria for integration into the Kennedy Space Center (KSC) Breadboard Project. The system consists of a 1 square meter plant growth area, a 500 liter fish culture tank, and computerized monitoring and control hardware. Nutrients in the hydrophonic solution were derived from fish metabolites and fish food leachate. In five months of continuous operation, 27.0 kg of lettuce tops, 39.9 kg of roots and biofilm, and 6.6 kg of fish (wet weights) were produced with 12.7 kg of fish food input. Based on dry weights, a biomass conversion index of 0.52 was achieved. A nitrogen budget was derived to determine partitioning of nitrogen within various compartments of the system. Accumulating nitrogen in the hypoponic solution indicated a need to enlarge the plant growth area, potentially increasing the biomass production and improving the biomass conversion index.

  10. Nitrogen and Phosphorus Removal in the Recirculating Aquaculture System with Water Treatment Tank containing Baked Clay Beads and Chinese Cabbage

    Aeknarin Thanakitpairin; Wiboonluk Pungrasmi; Sorawit Powtongsook

    2014-01-01

    This research aims to describe the nitrogen and phosphorus removal in Recirculating Aquaculture System (RAS) by crop plants biomass production. The 3 experiment systems consisted of 1 treatment (fish tank + baked clay beads + Chinese cabbage) and 2 controls as control-1 (fish tank only) and control-2 (fish tank + baked clay beads), were performed. With all experimental RAS, Nile tilapia (Oreochromis niloticus) was cultured at 2 kg/m3 density. The baked clay beads (8-16 mm in diameter) were fi...

  11. The influence of stocking density on the growth of common carp, Cyprinus carpio, in a recirculating aquaculture system

    Ionica Enache; Victor Cristea; Tudor Ionescu; Săndita Ion

    2011-01-01

    The paper presents some aspects regarding the influence of stocking density on the breeding of the common carp in a recirculating aquaculture system (RAS). The experiment covered a 30 dayperiod. It was conducted in four aquaculture tanks, 500 L/tank, in the recirculating aquaculture pilot system of “Dunarea de Jos” University of Galați. Two kind of stocking density variants were compared: 64 kg/m3 in V1 (B1, B3) and 32 kg/m3 in V2 (B2, B4). The stocking density was: 491 fish in B1, avera...

  12. The potential use of constructed wetlands in a recirculating aquaculture system for shrimp culture.

    Lin, Ying-Feng; Jing, Shuh-Ren; Lee, Der-Yuan

    2003-01-01

    A pilot-scale constructed wetland unit, consisting of free water surface (FWS) and subsurface flow (SF) constructed wetlands arranged in series, was integrated into an outdoor recirculating aquaculture system (RAS) for culturing Pacific white shrimp (Litopenaeus vannamei). This study evaluated the performance of the wetland unit in treating the recirculating wastewater and examined the effect of improvement in water quality of the culture tank on the growth and survival of shrimp postlarvae. During an 80-day culture period, the wetland unit operated at a mean hydraulic loading rate of 0.3 m/day and effectively reduced the influent concentrations of 5-day biochemical oxygen demand (BOD5, 24%), suspended solids (SS, 71%), chlorophyll a (chl-a, 88%), total ammonium (TAN, 57%), nitrite nitrogen (NO2-N, 90%) and nitrate nitrogen (NO3-N, 68%). Phosphate (PO4-P) reduction was the least efficient (5.4%). The concentrations of SS, Chl-a, turbidity and NO3-N in the culture tank water in RAS were significantly (Paquaculture system (CAS) that simulated static pond culture without wetland treatment. However, no significant difference (Psystems. At the end of the study, the harvest results showed that shrimp weight and survival rate in the RAS (3.8 +/-1.8 g/shrimp and 90%) significantly (Precirculating system. PMID:12663210

  13. Quantitative role of shrimp fecal bacteria in organic matter fluxes in a recirculating shrimp aquaculture system.

    Beardsley, Christine; Moss, Shaun; Malfatti, Francesca; Azam, Farooq

    2011-07-01

    Microorganisms play integral roles in the cycling of carbon (C) and nitrogen (N) in recirculating aquaculture systems (RAS) for fish and shellfish production. We quantified the pathways of shrimp fecal bacterial activities and their role in C- and N-flux partitioning relevant to culturing Pacific white shrimp, Penaeus (Litopenaeus) vannamei, in RAS. Freshly produced feces from P. vannamei contained 0.6-7 × 10(10) bacteria g(-1) dry wt belonging to Bacteroidetes (7%), Alphaproteobacteria (4%), and, within the Gammaproteobacteria, almost exclusively to the genus Vibrio (61%). Because of partial disintegration of the feces (up to 27% within 12 h), the experimental seawater became inoculated with fecal bacteria. Bacteria grew rapidly in the feces and in the seawater, and exhibited high levels of aminopeptidase, chitinase, chitobiase, alkaline phosphatase, α- and β-glucosidase, and lipase activities. Moreover, fecal bacteria enriched the protein content of the feces within 12 h, potentially enriching the feces for the coprophagous shrimp. The bacterial turnover time was much faster in feces (1-10 h) than in mature RAS water (350 h). Thus, shrimp fecal bacteria not only inoculate RAS water but also contribute to bacterial abundance and productivity, and regulate system processes important for shrimp health. PMID:21426366

  14. Integrated Multi-Trophic Recirculating Aquaculture System for Nile Tilapia (Oreochlomis niloticus

    Puchong Sri-uam

    2016-06-01

    Full Text Available Three densities of the sex-reversed male Nile tilapia, Oreochromis niloticus (20, 25, 50 fish/m3 were cultivated in an integrated multi-trophic recirculating aquaculture system (IMRAS that involves the ecological relationship between several living organisms, i.e., phytoplankton, zooplankton, and aquatic plants. The results indicated that, by providing proper interdependency between various species of living organisms, the concentrations of ammonia, nitrite, nitrate, and phosphate in the system were maintained below dangerous levels for Nile tilapia throughout the cultivation period. The highest wet weight productivity of Nile tilapia of 11 ± 1 kg was achieved at a fish density of 50 fish/m3. The aquatic plants in the treatment tank could effectively uptake the unwanted nitrogen (N and phosphorus (P compounds with the highest removal efficiencies of 9.52% and 11.4%, respectively. The uptake rates of nitrogen and phosphorus by aquatic plants could be ranked from high to low as: Egeria densa > Ceratophyllum demersum > Vallisneria spiralis and Vallisneria americana > Hygrophila difformis. The remaining N was further degraded through nitrification process, whereas the remaining P could well precipitate in the soil sediment in the treatment tank.

  15. Aquaculture Information Package

    Boyd, T.; Rafferty, K. [editors

    1998-01-01

    This package of information is intended to provide background to developers of geothermal aquaculture projects. The material is divided into eight sections and includes information on market and price information for typical species, aquaculture water quality issues, typical species culture information, pond heat loss calculations, an aquaculture glossary, regional and university aquaculture offices and state aquaculture permit requirements.

  16. Managing sea cucumber fisheries and aquaculture : Studies of social-ecological systems in the Western Indian Ocean

    Eriksson, Hampus

    2012-01-01

    Collecting sea cucumbers to supply the high value Chinese dried seafood market is a livelihood activity available to many people in the Western Indian Ocean (WIO), making it an important part of local economies. These fisheries are generally not successfully managed and tropical sea cucumber fisheries show continuing signs of decline. This thesis takes a social-ecological systems approach to guide better management of sea cucumber fisheries and aquaculture in the WIO. Papers 1 and 2 analyse t...

  17. Potential and limitations of ozone in marine recirculating aquaculture systems - Guidelines and thresholds for a safe application

    Schröder, Jan

    2011-01-01

    The aim of the present thesis was to assess the potential and limitations of ozonation in marine recirculating aquaculture systems (RAS) while particularly focussing on the toxicity, formation and removal of ozone-produced oxidants (OPO) in order to develop guidelines and thresholds for a reasonable and safe ozone application. In the first two chapters the toxicity of OPO was investigated for Pacific white shrimp (Litopenaeus vannamei) and turbot (Psetta maxima) and maximum safe exposure leve...

  18. The Effects of Some Phytobiotics on Biochemical Composition of Oreochromis Niloticus Meat Reared in a Recirculating Aquaculture System

    Alina Antache; Cristea Victor; Grecu Iulia; Dediu Lorena; Mocanu (Cretu) Mirela; Ion (Placinta) Sandita; Petrea Stefan Mihai

    2013-01-01

    The purpose of this experiment is the evaluation of biochemical composition of Nile tilapia meat by administrating  several phytobiotics in feed. Oreochromis niloticus, with an initial average weight of 125.41±34.33 g/exemplar, were reared, during six weeks in a recirculating aquaculture system. The phytobiotics from this experiment were administered in feed in a concentration of 1%/kg feed and consist in: thyme (Thymus vulgaris), fenugreek (Trigonela foenum graecum), neem (Azadirachta indica...

  19. Aquaculture, a Component of the Farming Systems among the Fishing Communities in Oil Producing Areas of Delta, Nigeria

    2013-01-01

    The study focused on ascertaining the relative importance of aquaculture in the farming systems of Oil Producing Areas Delta State, Nigeria. A stratified random sample of 598 households from 47 communities was used. Primary data collected between the months of May 2009 and February 2011 by means of structured questionnaire, complemented by oral were analysed using both descriptive and inferential statistics. The results show that 69% of the respondents were into fishing/fish farming either as...

  20. Broodstock management of the fine flounder Paralichthys adspersus (Steindachner, 1867 using recirculating aquaculture systems

    Lili Carrera

    2013-03-01

    Full Text Available The present study describes the methodology used at IMARPE for the capture, acclimation and management of P. adspersus broodstock using recirculating aquaculture systems (RAS. RAS improved the water quality and maintained the environmental parameters during the acclimation period, temperature (17.2±1°C, oxygen (8.1±0.7 mg L-1, pH (7.3±0.2, ammonia (0.004±0.003 mg L-1, nitrite (0.52±0.2 mg L-1 and nitrate (3.45±2.6 mg L-1. Fish began to be fed normally from day 15 post-capture, once or twice a day using live fish (Odonthestes regia regia, Mugil cephalus, crustacean (Emerita analoga, fresh food (Engraulis ringens and Dosidicus gigas and artificial feed. A significant loss in the weight of the fish was registered during the first days of captivity, followed by a continuous increase in both sexes. The specific growth rate was positive from the third month of captivity, being the relative growth rate 24.5% and 16.2% in August 2010 in males and females, respectively. Different internal and external parasites were detected in the fish, being Entobdella sp. and Philometra sp. the prevailing parasites observed during samplings.

  1. Observations on side-swimming rainbow trout in water recirculation aquaculture systems.

    Good, Christopher; Davidson, John; Kinman, Christin; Kenney, P Brett; Bæverfjord, Grete; Summerfelt, Steven

    2014-12-01

    During a controlled 6-month study using six replicated water recirculation aquaculture systems (WRASs), it was observed that Rainbow Trout Oncorhynchus mykiss in all WRASs exhibited a higher-than-normal prevalence of side swimming (i.e., controlled, forward swimming but with misaligned orientation such that the fish's sagittal axis is approximately parallel to the horizontal plane). To further our understanding of this abnormality, a substudy was conducted wherein side swimmers and normally swimming fish were selectively sampled from each WRAS and growth performance (length, weight), processing attributes (fillet yield, visceral index, ventrum [i.e., thickness of the ventral "belly flap"] index), blood gas and chemistry parameters, and swim bladder morphology and positioning were compared. Side swimmers were found to be significantly smaller in length and weight and had less fillet yield but higher ventrum indices. Whole-blood analyses demonstrated that, among other things, side swimmers had significantly lower whole-blood pH and higher Pco2. Side swimmers typically exhibited swim bladder malformations, although the positive predictive value of this subjective assessment was only 73%. Overall, this study found several anatomical and physiological differences between side-swimming and normally swimming Rainbow Trout. Given the reduced weight and fillet yield of market-age side swimmers, producers would benefit from additional research to reduce side-swimming prevalence in their fish stocks. PMID:25250476

  2. Membrane filtration coupled with chemical precipitation to treat recirculating aquaculture system effluents.

    Yang, Ling; Zhou, Hongde; Moccia, Richard

    2006-01-01

    Effluents from recirculating aquaculture systems (RAS) contain high concentrations of nitrogen and phosphorous wastes and thus often require proper treatment to prevent potential detrimental impacts on receiving water bodies. The purpose of this study was to evaluate the feasibility of membrane filtration coupled with chemical precipitation as a pretreatment step with emphasis on phosphorus removal from RAS effluents. Chemical precipitation tests were conducted by adding magnesium chloride and alum at different chemical concentrations and pH values, respectively. Crossflow, flat-sheet membrane filtration modules were used to examine the effects of transmembrane pressure and crossflow velocity in terms of solid/liquid separation efficiency and permeate flux decline. The results showed that membrane filtration can effectively separate the phosphorus precipitates after chemical precipitation. The total phosphorus in the treated effluent was reduced to less than 0.05 mg L(-1) with a removal efficiency of more than 90%. However, much lower removal efficiencies were obtained for total organic carbon (TOC), total nitrogen, and turbidity. It was concluded that membrane filtration coupled with chemical precipitation can become an effective, compact treatment technology to meet the stringent regulatory requirements for RAS effluent discharge. PMID:17071913

  3. Mathematical modelling of nutrient balance of a goldfish (Carassius auratus Linn. recirculating aquaculture system (GRAS

    Sudeep Puthravilakom Sadasivan Nair

    2010-08-01

    Full Text Available In the present study, a goldfish (Carassius auratus Linn. recirculating aquaculture system (GRAS has been developed. The GRAS consisted of a culture tank, a screen filter and a foam fractionator for removal of particulate and dissolved solids and a trickling filter for conversion of ammonium- and nitrite-nitrogen to relatively harmless nitrate-nitrogen. The culture of goldfish at a stocking density of 1.08 kg/m3 was continued for a period of two and half months. Based on mass balance analysis of ammonium- and nitrate-nitrogen and assuming the trickling filter to be a plug flow reactor, a model was formulated to determine the necessary recirculation flow rate at different times of culture for maintaining the major nutrients, viz., ammonium- and nitrate-nitrogen below their permissible limits. The model was calibrated and validated using the real time data obtained from the experimental run. The high values of coefficient of determination and low values of root mean square error show the effectiveness of the model.

  4. Conditioning of broodstock of tiger grouper, Epinephelus fuscoguttatus, in a recirculating aquaculture system

    Saleem Mustafa

    2015-11-01

    Full Text Available Closing the cycle of commercial species of fish in a recirculating aquaculture system is gaining importance for a number of practical advantages. Founder broodstock originating from the wild population is conditioned to live in hatchery tanks under suitable environmental and feeding conditions and is induced to breed. The juveniles are grown to maturity and facilitated to spawn in captivity to close the life cycle in the hatchery. This experiment was carried out on tiger grouper (Epinephelus fuscoguttatus. After preliminary observations, it was possible to identify appropriate environmental conditions in terms of water quality parameters, volume of broodstock tanks and ration. Growth was nearly isometric (growth exponent = 2.9185 and the condition factor = 1.86. This reflected good management conditions. Cues that trigger sex reversal in this protogynous fish in the hatchery were different from those that operate in nature. It appears that the differentiation of some individuals of a cohort into male sex is linked to socio-demographic cues as well as internal condition of the fish because it related to age and physiological condition. This view was reinforced by a lack of response in young fish to similar cues. The information generated through this study defines what is required for optimum conditioning of tiger grouper broodstock and explains the cues involved in sex differentiation.

  5. [Nitrate removal from recirculating aquaculture system using polyhydroxybutyrate-co-hydroxyvalerate as carbon source ].

    Zhang, Lanhe; Liu, Lili; Qiu, Tianlei; Gao, Min; Han, Meilin; Yuan, Ding; Wang, Xuming

    2014-09-01

    [ OBJECTIVE] Polyhydroxybutyrate-co-hydroxyvalerate (PHBV) was used as solid carbon source and biofilm carrier to remove nitrate from recirculating aquaculture system (RAS). Dynamics of microbial community structure in biofilm coating on carbon source packed into denitrification reactor were investigated. [METHODS] Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was used to analyze the microbial community in biofilm from denitrifiation reactor. Bacteria degrading PHBV were isolated from the reactor using pure culture method. [RESULTS] Nitrate decreased remarkably in the RAS connected with dentrification reactor. In contrast, Nitrate increased continuously in the conventional RAS without dentrification reactor. According to the phylogenetic analysis, the microbes in the biofilm samples from denitrification reactor were divided into Proteobacteria ( p-proteobacteria, γ-proteobacteria and δ- proteobacteria) , Firmicutes and Bacteroidetes. The major advantageous populations were Acidovorax and Bacillus in the 40-day reactor. The advantageous populations in the 150-day reactor were in order of Clostridium, Desulfitobacterium, Dechloromonas, Pseudoxanthomonas and Flavobacterium. Pure cultures of bacteria degrading PHBV isolated from denitrification reactor were classified into Acidovorax, Methylibium, Pseudoxanthomonas and Dechloromonas. [CONCLUSION] Nitrate could be removed effectively from RAS using PHBV as carbon source. Advantageous bacteria and their dynamic changes were ascertained in biofilm from denitrification reactor packed with PHBV. PMID:25522594

  6. Biomethanation and microbial community changes in a digester treating sludge from a brackish aquaculture recirculation system.

    Zhang, Xuedong; Tao, Yu; Hu, Jianmei; Liu, Gang; Spanjers, Henri; van Lier, Jules B

    2016-08-01

    Using a high-salinity-adapted inoculum and a moderate stepwise-increased organic loading rate (OLR), a stable digester performance was achieved in treating sludge from a brackish aquaculture recirculation system. The specific methane yield was distinctly enhanced, reaching 0.203LCH4/gCODadded, compared to literature values (0.140-0.154LCH4/gCODadded) from the salty sludges. OLR adjustment and the fecal substrate substantially influenced population changes in the digester. Within the bacterial subpopulations, the relative abundance of Bacillus and Bacteroides declined, accompanied by the increase of Clostridium and Trigonala over time. The results show Trigonala was derived from the substrate and accumulated inside the digester. The most abundant methanogen was Methanosarcina in the inoculum and the digestates. The Methanosarcina proliferation can be ascribed to its metabolic versatility, probably a feature of crucial importance for high-salinity environments. Other frequently observed methanogens were outcompeted. The population similarity at the genus level between inoculum and digestates declined during the initial stage and afterwards increased. PMID:27155261

  7. An integrated closed system for fish-plankton aquaculture in Amazonian fresh water.

    Gilles, S; Ismiño, R; Sánchez, H; David, F; Núñez, J; Dugué, R; Darias, M J; Römer, U

    2014-08-01

    A prototype of an integrated closed system for fish-plankton aquaculture was developed in Iquitos (Peruvian Amazonia) in order to cultivate the Tiger Catfish, Pseudoplatystoma punctifer (Castelnau, 1855). This freshwater recirculating system consisted of two linked sewage tanks with an intensive rearing unit (a cage) for P. punctifer placed in the first, and with a fish-plankton trophic chain replacing the filters commonly used in clear water closed systems. Detritivorous and zooplanktivorous fishes (Loricariidae and Cichlidae), maintained without external feeding in the sewage volume, mineralized organic matter and permitted the stabilization of the phytoplankton biomass. Water exchange and organic waste discharge were not necessary. In this paper we describe the processes undertaken to equilibrate this ecosystem: first the elimination of an un-adapted spiny alga, Golenkinia sp., whose proliferation was favored by the presence of a small rotifer, Trichocerca sp., and second the control of this rotifer proliferation via the introduction of two cichlid species, Acaronia nassa Heckel, 1840 and Satanoperca jurupari Heckel, 1840, in the sewage part. This favored some development of the green algae Nannochloris sp. and Chlorella sp. At that time we took the opportunity to begin a 3-month rearing test of P. punctifer. The mean specific growth rate and feed conversion ratio (FCR) of P. punctifer were 1.43 and 1.27, respectively, and the global FCR, including fish in the sewage part, was 1.08. This system has proven to be suitable for growing P. punctifer juveniles out to adult, and provides several practical advantages compared with traditional recirculating clear water systems, which use a combination of mechanical and biological filters and require periodic waste removal, leading to water and organic matter losses. PMID:24849417

  8. Importance of prebiotics in aquaculture as immunostimulants. Effects on immune system of Sparus aurata and Dicentrarchus labrax.

    Carbone, Donatella; Faggio, Caterina

    2016-07-01

    Infectious diseases in fish represent a major problem for the aquaculture field as they produce extensive damages and loss. Over the last few years, with increased development of the aquaculture industry, different methods have been used to contrast these pathologies. Common interest has led to the use of components (as additives in diets) that could contrast diseases without causing any negative impact on the environment. These components are represented by prebiotics, probiotics, and plant extracts. In this review, the effects of prebiotics are described. Prebiotics are indigestible fibres fermented by gut enzymes and commensal bacteria, whose beneficial effects are due to the by-products generated from fermentation. The influence of pre-biotics on the immune system of fish is called immunosaccharides. Mannanoligosaccharides (MOS), Fructooligosaccharides (FOS) and Inulin act at different levels in the innate immune response. For example, through phagocytosis, lysozyme activity, and the complement system activity, an increase in fish growth and an amelioration of their health status is brought about. In this review, the use of prebiotics in aquaculture, such as immunostimulants, has been highlighted: particularly in two teleost fish species, Sparus aurata and Dicentrarchus labrax. The results demonstrate that the road is still long and further studies are required, but the use of prebiotics, individually or coupled together, can open the doors to pioneering a new model of alternative components to antimicrobial agents. PMID:27074444

  9. Environmental sustainability assessment of a microalgae raceway pond treating aquaculture wastewater: From up-scaling to system integration.

    Sfez, Sophie; Van Den Hende, Sofie; Taelman, Sue Ellen; De Meester, Steven; Dewulf, Jo

    2015-08-01

    The environmental sustainability of aquaculture wastewater treatment by microalgal bacterial flocs (MaB-flocs) in an outdoor raceway pond was analyzed using life cycle assessment. Pikeperch aquaculture wastewater treated at pilot scale (Belgium; 28m(2)) and industrial scale (hypothetical up-scaling; 41 ponds of 245m(2)) were compared. The integration of the MaB-floc raceway pond in a broader aquaculture waste treatment system was studied, comparing the valorisation of MaB-flocs as shrimp feed and as biogas. Up-scaling improves the resource footprint of the plant (848MJex,CEENEkg(-1) MaB-floc TSS at pilot scale and 277MJex,CEENEkg(-1) MaB-floc TSS at industrial scale) as well as its carbon footprint and eutrophication potential. At industrial scale, the valorisation of MaB-flocs as shrimp feed is overall more sustainable than as biogas but improvements should be made to reduce the energy use of the MaB-floc raceway pond, especially by improving the energy-efficiency of the pond stirring system. PMID:25965258

  10. Improved water quality in recirculating aquaculture systems (RAS) by applying a membrane bioreactor (MBR) concept for removal of colloidal and fine suspended solids

    Holan, Astrid Buran

    2013-01-01

    The world’s human population is growing, and there is an increased demand for food. Aquaculture production is increasing rapidly to meet these demands and there is a growing interest for recirculating aquaculture systems (RAS) to maximize production. A typical RAS facility consists of production tanks and a water treatment compartment for removal or conversion of accumulated nutrients, organic matter and particulates where the treated water is recycled to the production tanks. Reuse of water ...

  11. Errors in experimental design and statistical analysis of aquaculture diet evaluation studies induced by filtration systems

    Michael F. Tlusty

    2010-07-01

    Full Text Available While single factor diet studies appear to be of simple design, the space constraints in aquaculture caused by the need to filter the aqueous media often c reate constraints on the implementation of the experimental design. A number of papers have been published in which the experimental design and subsequent analysis is incorrect. This paper reviews the principles of experimental design as it related to single factor diet evaluation studies in aquaculture. By adhering tosimple design principles to ensure that experimental units are independent, the data generated by future experiments will be of high quality and results more robust.

  12. Perceptions of shellfish aquaculture in British Columbia and implications for well-being in marine social-ecological systems

    Linda M. D'Anna

    2015-03-01

    Full Text Available Shellfish aquaculture is often positioned as an adaptive alternative to traditional resource industries, but the social and cultural effects of expanding production on coastal/marine social-ecological systems are unclear. Reporting on a multimethods study, we present perceptions about shellfish aquaculture collected through interviews, participant-employed photography, and a household survey in British Columbia, Canada. With an approach focused on local preferences for social-ecological conditions and the ways in which those conditions may be enhanced or diminished, we indicate that perceptions of the effects of aquaculture on the environment, economy, and lived experience are composed of both objective and subjective components. Interview responses and survey opinions varied widely and included bimodal responses. Industry interviewees tended to focus on environmental and economic benefits while acknowledging concerns about the environment and lived experience. Nonindustry interviewees typically questioned the environmental effects while underscoring economic benefits and negative effects on experience. Most survey participants felt positively about the effects on the economy, expressed negativity and uncertainty about effects on the environment, and demonstrated the greatest variability in opinions about effects on lived experience. Findings revealed uncertainty and alienation across all dimensions. Our findings, used as an analytical lens, support the usefulness of the concept of well-being in attempts like this one to understand the dynamics of coastal communities by providing a framework for deciphering what is important to individuals and societies experiencing change and considering adaptations.

  13. Identification of Bacterial Community Composition in Freshwater Aquaculture System Farming of Litopenaeus vannamei Reveals Distinct Temperature-Driven Patterns

    Yuyi Tang

    2014-08-01

    Full Text Available Change in temperature is often a major environmental factor in triggering waterborne disease outbreaks. Previous research has revealed temporal and spatial patterns of bacterial population in several aquatic ecosystems. To date, very little information is available on aquaculture environment. Here, we assessed environmental temperature effects on bacterial community composition in freshwater aquaculture system farming of Litopenaeus vannamei (FASFL. Water samples were collected over a one-year period, and aquatic bacteria were characterized by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE and 16S rDNA pyrosequencing. Resulting DGGE fingerprints revealed a specific and dynamic bacterial population structure with considerable variation over the seasonal change, suggesting that environmental temperature was a key driver of bacterial population in the FASFL. Pyrosequencing data further demonstrated substantial difference in bacterial community composition between the water at higher (WHT and at lower (WLT temperatures in the FASFL. Actinobacteria, Proteobacteria and Bacteroidetes were the highest abundant phyla in the FASFL, however, a large number of unclassified bacteria contributed the most to the observed variation in phylogenetic diversity. The WHT harbored remarkably higher diversity and richness in bacterial composition at genus and species levels when compared to the WLT. Some potential pathogenenic species were identified in both WHT and WLT, providing data in support of aquatic animal health management in the aquaculture industry.

  14. Inland marine fish culture in low-salinity recirculating aquaculture systems

    Expansion of marine aquaculture is challenged by the high cost and limited availability of coastal land and water resources, effluent concerns, high production costs, restricted growing seasons, lack of quality seedstock, and inadequate regulatory and permitting processes. Many of these constraints...

  15. Nutrient Discharge from aquaculture operations in function of system design and production enviorment

    Verdegem, M.C.J.

    2013-01-01

    In aquaculture, nutrient loading is defined as the difference between nutrients supplied with fertilizers and feed and nutrients harvested in the form of finfish, crustaceans, molluscs and seaweeds. On average, the production of finfish and crustaceans results in a net nutrient loading, while for th

  16. Removal of nitrogen by Algal Turf Scrubber Technology in recirculating aquaculture system

    Valeta, J.; Verdegem, M.C.J.

    2015-01-01

    Ongoing research in recirculation aquaculture focuses on evaluating and improving the purification potential of different types of filters. Algal Turf Scrubber (ATS) are special as they combine sedimentation and biofiltration. An ATS was subjected to high nutrient loads of catfish effluent to examin

  17. Performance of Nile tilapia Oreochromis niloticus fingerlings in a hyper-intensive recirculating aquaculture system with low water exchange

    M Gullian-Klanian; C Arámburu-Adame

    2013-01-01

    The aim of this paper was evaluate the performance of Nile tilapia fingerlings (Oreochromis niloticus) raised at hyper intensive stocking density in a recirculating aquaculture system (RAS) with minimum water replacing. The experimental system was performed in a single-batch nursery system to obtain 50 g fish size in 60 days. Fish (2.07 ± 0.04 g) were stocked in triplicate at 400 (T1), 500 (T2) and 600 (T3) fish m-3 (0.84, 1.05, 1.22 kg m-3). RAS functioned with 12,000 L of recirculating wate...

  18. The influence of pellets quality on the growth of sterlet, in recirculating aquaculture system

    Corina Sion

    2011-04-01

    Full Text Available The paper presents some aspects regarding the influence of food quality on the breeding of sterlet, Acipenser ruthenus Linnaeus, 1758, in a recirculating aquaculture system (RAS. The experiment took place over 30 days, in four pilot breeding units type aquaria of 300 liters in volume. Two kind of variants were compared, with repetition, V1 with 46% crude protein and V2 with 30% crude protein, respectively. The stocking density was of 14 fish/unit. The same feeding level of 8 g/kg metabolic weight (1.5% from total biomass was used in every unit. The technological indicators that showed up at the end of the experiment revealed the following: the mean biomass gain in V1 was of 0.74 kg/m3 compared to 0.39 kg/m3in V2; this was nearly perfectly correlated with the food quality. The growth rate (GR variated from 6.70 to 8.13 g/day in V1 and 3.63-4.17 g/day in V2; the specific growth rate (SGR, calculated as a mean value of the two repetitions of each variant, was of 1.11 g%/day in V1 and 0.63 g%/day in V2; the feed conversion ratio (FCR, calculated as the mean value of the two repetitions from the two variants, was of 1.49 in V1 and of 2.81 in V2. The parameters of fish breeding showed that changing the quality of fodder, the fish growing was positively influenced. This experiment showed also that sterlet is a sturgeon with a moderate growth rate and it is possible to obtain an increase of fish biomass using pellets with 30-46 % crude protein.

  19. Optimizing Hydraulic Retention Times in Denitrifying Woodchip Bioreactors Treating Recirculating Aquaculture System Wastewater.

    Lepine, Christine; Christianson, Laura; Sharrer, Kata; Summerfelt, Steven

    2016-05-01

    The performance of wood-based denitrifying bioreactors to treat high-nitrate wastewaters from aquaculture systems has not previously been demonstrated. Four pilot-scale woodchip bioreactors (approximately 1:10 scale) were constructed and operated for 268 d to determine the optimal range of design hydraulic retention times (HRTs) for nitrate removal. The bioreactors were operated under HRTs ranging from 6.6 to 55 h with influent nitrate concentrations generally between 20 and 80 mg NO-N L. These combinations resulted in N removal rates >39 g N m d, which is greater than previously reported. These high removal rates were due in large part to the relatively high chemical oxygen demand and warm temperature (∼19°C) of the wastewater. An optimized design HRT may not be the same based on metrics of N removal rate versus N removal efficiency; longer HRTs demonstrated higher removal efficiencies, and shorter HRTs had higher removal rates. When nitrate influent concentrations were approximately 75 mg NO-N L ( = 6 sample events), the shortest HRT (12 h) had the lowest removal efficiency (45%) but a significantly greater removal rate than the two longest HRTs (42 and 55 h), which were N limited. Sulfate reduction was also observed under highly reduced conditions and was exacerbated under prolonged N-limited environments. Balancing the removal rate and removal efficiency for this water chemistry with a design HRT of approximately 24 h would result in a 65% removal efficiency and removal rates of at least 18 g N m d. PMID:27136146

  20. Trophic structure in a pilot system for the integrated multi-trophic aquaculture off the east coast of Korean peninsula as determined by stable isotopes

    Highlights: • A pilot system for the integrated multi-trophic aquaculture was installed. • The black rockfish, sea cucumber, oyster, and macroalgae were co-cultured. • Co-cultured sea cucumber at the IMTA site assimilated aquaculture wastes. • Fish cage-waste contribution to food web in/around the IMTA system was minor. • The sea cucumber was identified as an extractive species of fish cage wastes. - Abstract: To assess the potential for nutritional exploitation of caged-fish-derived waste through the use of extractive co-cultured species in a pilot system for an integrated multi-trophic aquaculture (IMTA), we compared their C and N stable isotope ratios with those of uncultured macroinvertebrates in and around the system. Black rockfish were co-cultured with sea cucumber, oyster, and two macroalgae as extractive species. Isotope signatures of the co-cultured sea cucumber at the IMTA site differed from those at the control site, indicating their assimilation of aquaculture wastes. In contrast, δ13C and δ15N of individual taxa of the cultured oyster and uncultured invertebrates were consistent between sites, suggesting a minor contribution of the aquaculture waste to benthic and pelagic food chains in and around the IMTA system. These results provide evidence of the suitability of using sea cucumber as an extractive species to reduce the impact of a monoculture system on the ambient environment

  1. ECONOMIC ANALYSIS OF FRESHWATER AQUACULTURE PRODUCTION: A COMPARATIVE ANALYSIS OF DIFFERENT PRODUCTION SYSTEMS

    H. Kumar

    2013-01-01

    Full Text Available India produced 8.29 million tonnes of fish in 2010-2011. The industry contributes nearly INR 200 trillion to the national economy, forming 1.4 percent of national gross domestic product (GDP and 5.4 percent of Agricultural GDP. At present, almost 84 percent of the total inland fish production, in the country is contributed by freshwater aquaculture amounting to 3.9 million tonnes in 2008-09. Further, the potential of the vast freshwater resources covering 6.7 million hectare is yet to be fully realized. The freshwater aquaculture which began as small scale activity of stocking ponds with fish seed collected from riverine sources during early fifties in rural Bengal has now transformed into a major economic activity in almost all states. There is a further need to make the sector more vibrant so as to achieve the predicted target of 15 kg per capita fish availability in the country by 2030.

  2. Peracetic acid degradation in freshwater aquaculture systems and possible practical implications

    Pedersen, Lars-Flemming; Meinelt, Thomas; Straus, David L.

    2013-01-01

    Peracetic acid (PAA) is a highly reactive peroxygen compound with wide-ranging antimicrobial effects and is considered an alternative sanitizer to formaldehyde. Products containing PAA are available in solution with acetic acid and hydrogen peroxide to maintain the stability of the chemical, and ......, documenting a large discrepancy between delivered quantities and realized residuals. Recent investigations of PAA application to manage parasitic diseases in aquaculture are briefly reviewed, and practical implication and guidelines are addressed...

  3. Fluoroquinolones and Tetracycline Antibiotics in a Portuguese Aquaculture System and Aquatic Surroundings: Occurrence and Environmental Impact.

    Pereira, André M P T; Silva, Liliana J G; Meisel, Leonor M; Pena, Angelina

    2015-01-01

    The growth of aquaculture over the past few years is widely recognized as one of the main sources of antibiotics, mainly fluoroquinolones (FQ) and tetracyclines (TC), in the aquatic environment, consequently, increasing the risk of the emergence of antibiotic bacterial resistance and promoting the spread of resistant genes. This study aimed to (1) develop and validate a multiresidue method for determination and quantification of ciprofloxacin (CIP), difloxacin (DIFL), enrofloxacin (ENR), norfloxacin (NOR), sarafloxacin (SARA), and oxytetracycline (OXY) in aquaculture waters and surrounding water bodies and (2) provide the first Portuguese data to utilize in assessment of risk of adverse effects. In addition, the potential environmental impact posed by these antibiotics to aquatic organisms, belonging to different trophic levels, when exposed to the studied aquaculture waters was also assessed. The analytical strategy comprised of solid-phase extraction (SPE) through Oasis HLB cartridges, and detection and quantification by liquid chromatography with tandem mass spectrometry (LC/MS(n)). Method detection limits (MDL) and method quantification limits (MQL) were in the range of 0.7-3 ng/L and 2.4-10 ng/L, respectively. Recoveries varied between 57.4 and 122.8%. The method was applied to 31 water samples collected from an aquaculture and surrounding water bodies located in north of Portugal. Residues of all antibiotics, except SARA and DIFL, were detected at concentrations ranging from 3 to 75.1 ng/L. Norfloxacin was the antibiotic present at highest frequency and concentration. Regarding the environmental impact assessment (EIA), a risk quotient higher than 1 was observed for NOR. PMID:26262440

  4. Comparison of Atlantic salmon net pen and recirculating aquaculture systems: economical, technological and environmental issues

    Dekhtyarev, Vitaly

    2014-01-01

    The modern aquaculture industry is a rapidly developing sector of the fisheries industry. Among the fish species reared in marine waters Atlantic salmon (Salmo salar) shares a significant part. Nowadays, the largest salmon producing countries are Norway, Chile and Scotland. The common technology used in the salmon production is a sea cage, which is presented in a form of floating plastic rings or robust metal installations fastened to a barge. In both cases, the fish is placed ...

  5. Feed alternatives and phosphorus efficiency of the Norwegian fisheries and aquaculture system

    Vestrum, Magnus Inderberg

    2013-01-01

    With the worlds reserves of rock phosphate expected to be depleted within 50-100 years, the mapping of the flows of phosphorus in the world is crucial. The Norwegian fisheries and aquaculture industry is one of the largest industries in Norway, and is predicted to grow significantly the coming decades. This opens up to many challenges and opportunities regarding sustainability and efficient use of the available resources. In this thesis a material flow analysis (MFA) is utilized in order to i...

  6. Aquaculture information package

    Boyd, T.; Rafferty, K.

    1998-08-01

    This package of information is intended to provide background information to developers of geothermal aquaculture projects. The material is divided into eight sections and includes information on market and price information for typical species, aquaculture water quality issues, typical species culture information, pond heat loss calculations, an aquaculture glossary, regional and university aquaculture offices and state aquaculture permit requirements. A bibliography containing 68 references is also included.

  7. Electron beam focusing system

    Dikansky, N.; Nagaitsev, S.; Parkhomchuk, V.

    1997-09-01

    The high energy electron cooling requires a very cold electron beam. Thus, the electron beam focusing system is very important for the performance of electron cooling. A system with and without longitudinal magnetic field is presented for discussion. Interaction of electron beam with the vacuum chamber as well as with the background ions and stored antiprotons can cause the coherent electron beam instabilities. Focusing system requirements needed to suppress these instabilities are presented.

  8. Effectiveness of Floating Micro-Bead Bio-Filter for Ornamental Fish in a Re-Circulating Aquaculture System

    R Fadhil

    2011-01-01

    Bio-filtration has been widely used in re-circulating aquaculture system to remove waste and to convert toxic ammonia andnitrite into safe end products ornamental fish and other aquatic organisms. However, the study of micro-bead usage as the filter medium has not yet been broadened and thoroughly developed. Therefore, the aim of this study is to construct a biological filter made from polyethylene micro-bead as the filter medium and to analyze its effectiveness in removingwaste as well as in...

  9. Effect of stocking density on performances of juvenile turbot (Scophthalmus maximus) in recirculating aquaculture systems

    LI Xian; LIU Ying; Jean-Paul BLANCHETON

    2013-01-01

    Limited information has been available about the influence of loading density on the performances of Scophthalmus maximus,especially in recirculating aquaculture systems (RAS).In this study,turbot (13.84±2.74 g; average weight±SD) were reared at four different initial densities (low 0.66,medium 1.26,sub-high 2.56,high 4.00 kg/m2) for 10 weeks in RAS at 23±1℃.Final densities were 4.67,7.25,14.16,and 17.47 kg/m2,respectively,which translate to 82,108,214,and 282 percent coverage of the tank bottom.Density had both negative and independent impacts on growth.The final mean weight,specific growth rate (SGR),and voluntary feed intake significantly decreased and the coefficient of variation (CV) of final body weight increased with increase in stocking density.The medium and sub-high density groups did not differ significantly in SGR,mean weight,CV,food conversion rate (FCR),feed intake,blood parameters,and digestive enzymes.The protease activities of the digestive tract at pH 7,8.5,9,and 10 were significantly higher for the highest density group,but tended to be lower (not significantly) at pH 4 and 8.5for the lowest density group.The intensity of protease activity was inversely related to feed intake at the different densities.Catalase activity was higher (but not significantly) at the highest density,perhaps because high density started to induce an oxidative effect in turbot.In conclusion,turbot can be cultured in RAS at a density of less than 17.47 kg/m2.With good water quality and no feed limitation,initial density between 1.26 and 2.56 kg/m2 (final:7.25 and 14.16 kg/m2) would not negatively affect the turbot cultured in RAS.For culture at higher density,multi-level feeding devices are suggested to ease feeding competition.

  10. Effect of stocking density on performances of juvenile turbot ( Scophthalmus maximus) in recirculating aquaculture systems

    Li, Xian; Liu, Ying; Blancheton, Jean-Paul

    2013-05-01

    Limited information has been available about the influence of loading density on the performances of Scophthalmus maximus, especially in recirculating aquaculture systems (RAS). In this study, turbot (13.84±2.74 g; average weight±SD) were reared at four different initial densities (low 0.66, medium 1.26, sub-high 2.56, high 4.00 kg/m2) for 10 weeks in RAS at 23±1°C. Final densities were 4.67, 7.25, 14.16, and 17.47 kg/m2, respectively, which translate to 82, 108, 214, and 282 percent coverage of the tank bottom. Density had both negative and independent impacts on growth. The final mean weight, specific growth rate (SGR), and voluntary feed intake significantly decreased and the coefficient of variation (CV) of final body weight increased with increase in stocking density. The medium and sub-high density groups did not differ significantly in SGR, mean weight, CV, food conversion rate (FCR), feed intake, blood parameters, and digestive enzymes. The protease activities of the digestive tract at pH 7, 8.5, 9, and 10 were significantly higher for the highest density group, but tended to be lower (not significantly) at pH 4 and 8.5 for the lowest density group. The intensity of protease activity was inversely related to feed intake at the different densities. Catalase activity was higher (but not significantly) at the highest density, perhaps because high density started to induce an oxidative effect in turbot. In conclusion, turbot can be cultured in RAS at a density of less than 17.47 kg/m2. With good water quality and no feed limitation, initial density between 1.26 and 2.56 kg/m2 (final: 7.25 and 14.16 kg/m2) would not negatively affect the turbot cultured in RAS. For culture at higher density, multi-level feeding devices are suggested to ease feeding competition.

  11. Osnovy formirovanija sistemy upravlenija riskami proektov innovacionnogo razvitija akvakul'tury v regione (Na primere Kaliningradskoj oblasti) [The formation of a risk management system for projects in the field of aquaculture innovative development in the Kaliningrad region: a case study

    Serbulov Alexey; Stepanov Alexey; Polyakov Oleg

    2012-01-01

    This article sets out to develop the concept and the principal scheme of the formation of a risk management system for innovative economic development projects in the field of aquaculture. The research carried out by the authors helps identify the main problems and characteristics of risk management projects for the development of aquaculture in presentday Russia. The authors outline the status and features of aquaculture development projects in the North-western federal district and the Kali...

  12. Anaerobic ammonium-oxidizing (anammox) bacteria and associated activity in fixed-film biofilters of a marine recirculating aquaculture system.

    Tal, Yossi; Watts, Joy E M; Schreier, Harold J

    2006-04-01

    Microbial communities in the biological filter and waste sludge compartments of a marine recirculating aquaculture system were examined to determine the presence and activity of anaerobic ammonium-oxidizing (anammox) bacteria. Community DNA was extracted from aerobic and anaerobic fixed-film biofilters and the anaerobic sludge waste collection tank and was analyzed by amplifying 16S rRNA genes by PCR using anammox-selective and universal GC-clamped primers. Separation of amplified PCR products by denaturing gradient gel electrophoresis and sequencing of the different phylotypes revealed a diverse biofilter microbial community. While Planctomycetales were found in all three communities, the anaerobic denitrifying biofilters contained one clone that exhibited high levels of sequence similarity to known anammox bacteria. Fluorescence in situ hybridization studies using an anammox-specific probe confirmed the presence of anammox Planctomycetales in the microbial biofilm from the denitrifying biofilters, and anammox activity was observed in these biofilters, as detected by the ability to simultaneously consume ammonia and nitrite. To our knowledge, this is the first identification of anammox-related sequences in a marine recirculating aquaculture filtration system, and our findings provide a foundation for incorporating this important pathway for complete nitrogen removal in such systems. PMID:16597996

  13. Impact of depuration of earth-musty off-flavors on fillet quality of atlantic salmon, Salmo salar, cultured in a recirculating aquaculture system

    Over the past decade in the United States, there has been increased interest in the establishment and use of land-based, closed-containment systems [e.g., recirculating aquaculture system (RAS)] for salmonid culture. These culture systems have unique challenges compared to net pen culture of salmon...

  14. Animal protein production modules in biological life support systems: Novel combined aquaculture techniques based on the closed equilibrated biological aquatic system (C.E.B.A.S.)

    Blüm, V.; Andriske, M.; Kreuzberg, K.; Schreibman, M. P.

    Based on the experiences made with the Closed Equilibrated Biological Aquatic System (C.E.B.A.S.) which was primarily deveoloped for long-term and multi-generation experiments with aquatic animals and plants in a space station highly effective fresh water recycling modules were elaborated utilizing a combination of ammonia oxidizing bacteria filters and higher plants. These exhibit a high effectivity to eliminate phosphate and anorganic nitrogen compounds and arc. in addidition. able to contribute to the oxygen supply of the aquatic animals. The C.E.B.A.S. filter system is able to keep a closed artificial aquatic ecosystem containing teleost fishes and water snails biologically stable for several month and to eliminate waste products deriving from degraded dead fishes without a decrease of the oxygen concentration down to less than 3.5 mg/l at 25 °C. More advanced C.E.B.A.S. filter systems, the BIOCURE filters, were also developed for utilization in semiintensive and intensive aquaculture systems for fishes. In fact such combined animal-plant aquaculture systems represent highly effective productions sites for human food if proper plant and fish species are selected The present papers elucidates ways to novel aquaculture systems in which herbivorous fishes are raised by feeding them with plant biomass produced in the BIOCURE filters and presents the scheme of a modification which utilizes a plant species suitable also for human nutrition. Special attention is paid to the benefits of closed aquaculture system modules which may be integrated into bioregenerative life support systems of a higher complexity for, e. g.. lunar or planetary bases including some psychologiccal aspects of the introduction of animal protein production into plant-based life support systems. Moreover, the basic reproductive biological problems of aquatic animal breeding under reduced gravity are explained leading to a disposition of essential research programs in this context.

  15. Characterizing the structural diversity of a bacterial community associated with filter materials in recirculating aquaculture systems of Scortum barcoo.

    Zhu, Peng; Ye, Yangfang; Pei, Fangfang; Lu, Kaihong

    2012-03-01

    The bacterial community structure associated with filter materials in the recirculating aquaculture system of Scortum barcoo was investigated using the 16S rRNA gene clone library method. Preliminary results showed that the clone library constructed from the initial operation condition was characterized by 31 taxa of bacteria belonging to eight phyla including Proteobacteria, Acidobacteria, Firmicutes, Fusobacteria, Sphingobacteria, Bacteroidetes, Verrucomicrobiae, and Actinobacteria. There were 14 taxa of bacteria belonging to four phyla including Proteobacteria, Acidobacteria, Planctomycetacia, and Nitrospirae from the stable operation condition where the water quality was well maintained. Nitrospirae was only found under the stable operation condition in this study. Our results further indicated that Nitrospira was dominated by members of the Nitrospira sp. lineages, with a minor fraction related to Nitrospira moscoviensis and an unknown Nitrospira cluster. These great differences of both diversity and composition between two operation conditions suggested that the composition of the microbial community varied with the degree of water quality in the recirculating aquaculture system of S. barcoo. PMID:22339297

  16. Effectiveness of Floating Micro-Bead Bio-Filter for Ornamental Fish in a Re-Circulating Aquaculture System

    R Fadhil

    2011-01-01

    Full Text Available Bio-filtration has been widely used in re-circulating aquaculture system to remove waste and to convert toxic ammonia andnitrite into safe end products ornamental fish and other aquatic organisms. However, the study of micro-bead usage as the filter medium has not yet been broadened and thoroughly developed. Therefore, the aim of this study is to construct a biological filter made from polyethylene micro-bead as the filter medium and to analyze its effectiveness in removingwaste as well as in converting the toxic organic matter into stable substances. The bio-filter was constructed under a rotational molding process. The tubes, hoses, and piping were made from polyvinyl chloride (PVC while the fasteners were made from stainless steel and other non-corrosive materials. The effectiveness of this bio-filter was measured by using biochemical oxygen demand (BOD and total suspended solids (TSS analysis. Results indicated that this bio-filter is efficient enough to remove suspended solids and BOD. Therefore, this floating micro-bead bio-filter can be used in aquaculture systems.

  17. Osnovy formirovanija sistemy upravlenija riskami proektov innovacionnogo razvitija akvakul'tury v regione (Na primere Kaliningradskoj oblasti [The formation of a risk management system for projects in the field of aquaculture innovative development in the Kaliningrad region: a case study

    Serbulov Alexey

    2012-01-01

    Full Text Available This article sets out to develop the concept and the principal scheme of the formation of a risk management system for innovative economic development projects in the field of aquaculture. The research carried out by the authors helps identify the main problems and characteristics of risk management projects for the development of aquaculture in presentday Russia. The authors outline the status and features of aquaculture development projects in the North-western federal district and the Kaliningrad region. The article formulates and justifies the concept of “risk management projects in innovative development of aquaculture in the region” focusing on the classification of aquaculture risks in relation to innovative development projects, which expands the conceptual framework of risk management in view of the specific risks relating to economic development projects in the field of aquaculture. The authors characterize modern methods and approaches to risk management projects and organizations in the context of their application in the framework of aquaculture development projects and offer mechanisms for risk management of aquaculture development projects, which make it possible to include risk management activity in the general context of activities of parent project organizations. The authors develop the concept and principal scheme of the formation of risk management system for innovative development projects in aquaculture.

  18. The influence of different stocking densities on biochemical composition of rainbow trout meat reared in a recirculating aquaculture system

    Mirela Cretu

    2014-05-01

    Full Text Available The aim of this study is to evaluate the biochemical composition of rainbow trout meat, reared in a recirculating aquaculture system, at different stocking densities (DS1-2,64 kg/m3, DS2-5,16 kg/m3, DS3-7,12 kg/m3, DS4-9,42 kg/m3. The analyses were performed in the research laboratory of Aquaculture, Environmental Science and Cadastre Department – Faculty of Food Science and Engineering, from ,,Dunarea de Jos’’University of Galati. The content of meat proteins, fats, dry substance and ash was determined. After a 33 days experimental trial, the biochemical analysis of meat from all four experimental variants shows that protein content was higher in DS1 and DS4 variants, while the smallest amount of fats was quantified at DS1 and DS2 variants, without significant differences (p >0.05 for none of the biochemical compounds analyzed, at the mean comparison on experimental variants. Thus, it can be concluded that biochemical composition of rainbow trout meat was not influenced by the tested stocking densities.

  19. Particle size distribution and removal efficiency for three types of clarifiers in a warm-water, low-salinity, recirculating aquaculture system for juvenile red drum culture

    Recirculating aquaculture systems offer a solution to declining natural finfish stocks while limiting nutrient outputs and eutrophication of effluent waters. Because water is reused in these systems, the solids must be efficiently removed by mechanical treatment devices otherwise the solids buildup...

  20. Foam fractionation efficiency for particulate matter removal from a low salinity, lowhead recirculating aquaculture system utilized for the culture of red drum, Sciaenops ocellatus

    In recirculating aquaculture systems (RAS), the water which is treated and reused must be of such quality to maintain the culture organism in a healthy and fast growing condition, especially as such systems are intensified. Managing particulate matter and different chemical compounds is a key factor...

  1. Enhancement of Anaerobic Digestion to Treat Saline Sludge from Recirculating Aquaculture Systems

    Guo-zhi Luo

    2015-01-01

    Full Text Available The effectiveness of carbohydrate addition and the use of ultrasonication as a pretreatment for the mesophilic anaerobic digestion of saline aquacultural sludge was assessed. Analyses were conducted using an anaerobic sequencing batch reactor (ASBR, which included stopped gas production attributed to the saline inhibition. After increasing the C : N ratio, gas production was observed, and the total chemical oxygen demand (TCOD removal efficiency increased from 75% to 80%. The TCOD removal efficiency of the sonication period was approximately 85%, compared to 75% for the untreated waste. Ultrasonication of aquaculture sludge was also found to enhance the gas production rate and the TCOD removal efficiency. The average volatile fatty acid (VFA to alkalinity ratios ranged from 0.1 to 0.05, confirming the stability of the digesters. Furthermore, soluble chemical oxygen demand (SCOD, VFA, and PO43- concentrations increased in the effluents. There was a 114% greater gas generation during the ultrasonication period, with an average production of 0.08 g COD/L·day−1.

  2. Enhancement of Anaerobic Digestion to Treat Saline Sludge from Recirculating Aquaculture Systems

    Luo, Guo-zhi; Ma, Niannian; Li, Ping; Tan, Hong-xin; Liu, Wenchang

    2015-01-01

    The effectiveness of carbohydrate addition and the use of ultrasonication as a pretreatment for the mesophilic anaerobic digestion of saline aquacultural sludge was assessed. Analyses were conducted using an anaerobic sequencing batch reactor (ASBR), which included stopped gas production attributed to the saline inhibition. After increasing the C : N ratio, gas production was observed, and the total chemical oxygen demand (TCOD) removal efficiency increased from 75% to 80%. The TCOD removal efficiency of the sonication period was approximately 85%, compared to 75% for the untreated waste. Ultrasonication of aquaculture sludge was also found to enhance the gas production rate and the TCOD removal efficiency. The average volatile fatty acid (VFA) to alkalinity ratios ranged from 0.1 to 0.05, confirming the stability of the digesters. Furthermore, soluble chemical oxygen demand (SCOD), VFA, and PO43− concentrations increased in the effluents. There was a 114% greater gas generation during the ultrasonication period, with an average production of 0.08 g COD/L·day−1. PMID:26301258

  3. An Overview of Aquaculture in the Nordic Countries

    Paisley, Larry; Ariel, Ellen; Lyngstad, T. M.;

    2010-01-01

    The goal of this review was to describe in some detail the Nordic aquaculture industries in order to illuminate the similarities and differences. Information that was gathered for each country includes aquaculture history, aquaculture acts and regulations, production and production systems, envir...... human consumption, significant numbers of fish are grown for restocking of rivers, lakes, or other bodies of freshwater or seawater.......The goal of this review was to describe in some detail the Nordic aquaculture industries in order to illuminate the similarities and differences. Information that was gathered for each country includes aquaculture history, aquaculture acts and regulations, production and production systems....... Arctic char and cod are most important in Iceland. Other important cultured species include eel and blue mussels. There is much diversity in Nordic aquaculture industries in terms of production, farmed species, and production systems. Although the vast majority of the Nordic aquaculture production is for...

  4. The impact of water exchange rate and treatment processes on water-borne hormones in recirculation aquaculture systems containing sexually maturing Atlantic salmon Salmo salar

    A controlled seven-month study was conducted in six replicated water recirculation aquaculture systems (WRAS) to assess post-smolt Atlantic salmon (Salmo salar) performance in relation to WRAS water exchange rate. Unexpectedly high numbers of precocious sexually mature fish were observed in all WRAS...

  5. Evaluation of depuration procedures to mitigate off-flavor from harvest size Atlantic Salmon Salmo Salar cultured in a land-based recirculating aquaculture system

    Fish cultured within water recirculating aquaculture systems (RAS) can develop “earthy” or “musty” off-flavors in their flesh due to the bioaccumulation of the compounds geosmin and 2-methylisoborenol (MIB), respectively, that are produced by certain bacteria (e.g., actinomycetes). These bacteria an...

  6. Comparing the effects of high vs. low nitrate on the health, performance, and welfare of juvenile rainbow trout Oncorhynchus mykiss within water recirculating aquaculture systems

    Previous research indicates that rainbow trout (Oncorhynchus mykiss) begin to exhibit health and welfare problems when cultured within water recirculating aquaculture systems (WRAS) operated at low exchange (6.7 days hydraulic retention time) and a mean feed loading rate of 4.1 kg feed/m3 daily make...

  7. Production characteristics of body composition of Florida pompano reared to market size at two different densities in low salinity recirculating aquaculture systems

    The effect of culture density on production characteristics and body composition of Florida pompano Trachinotus carolinus reared to market size using recirculating aquaculture systems (RAS) at a salinity of 5 g/L was evaluated in a 110 day growth trial (water temperature, 27.0-28.5 C). Juvenile pom...

  8. Evaluation of ozonation on levels of the off-flavor compounds geosmin and 2-methylisoborneol in water and rainbow trout Oncorhynchus mykiss from water recirculation aquaculture systems

    Common “off-flavors” in fish cultured in water recirculation aquaculture systems (WRAS) are “earthy” and “musty” due to the presence of the off-flavor metabolites geosmin and 2-methylisoborneol (MIB), respectively. Previously, ozone addition has been applied to WRAS at relatively low doses to break...

  9. Stressing fish in Recirculating Aquaculture Systems (RAS): Does stress induced in one group of fish affect the feeding motivation of other fish sharing the same RAS?

    Martins, C.I.; Eding, E.H.; Verreth, J.A.J.

    2011-01-01

    As a consequence of water re-use and high stocking densities, Recirculating Aquaculture Systems (RAS) may lead to an accumulation of substances released by the fish into the water, e.g. cortisol and alarm pheromones. This study investigated the effect of stressing fish on the feeding motivation of o

  10. The influence of stocking density on the growth of common carp, Cyprinus carpio, in a recirculating aquaculture system

    Ionica Enache

    2011-04-01

    Full Text Available The paper presents some aspects regarding the influence of stocking density on the breeding of the common carp in a recirculating aquaculture system (RAS. The experiment covered a 30 dayperiod. It was conducted in four aquaculture tanks, 500 L/tank, in the recirculating aquaculture pilot system of “Dunarea de Jos” University of Galați. Two kind of stocking density variants were compared: 64 kg/m3 in V1 (B1, B3 and 32 kg/m3 in V2 (B2, B4. The stocking density was: 491 fish in B1, average weight - 65 g/ fish; 245 fish in B2, average weight - 66 g/ fish; 211 fish in B3, average weight - 152 g/fish; 107 fish in B4, average weight - 150 g/fish. The technological indicators obtained revealed the following: the specific growth rate (SGR, calculated as a mean value on the two repetitions, was 1.28%/day in V1 and 1.49 %/day in V2, indicating better growth in V2; the food conversion ratio (FCR, calculated as the mean value of the two repetitions, was 1.28 in V1 and 1.06 in V2, expressing a higher efficiency in capitalization of food in V2. The mean biomass gain in V1 was 30.46 kg/m3 as compared to 18.55 kg/m3 in V2, almost perfectly correlated with the stocking density. The daily growth rate (DGR varied in a similar way, from 494.57 to 520.90 g/day in V1 and 302.4 to 315.9 g/day in V2. The experiment demonstrated a remarkable technological plasticity in both experimental variants, whereas, in terms of technological performance indicators, it was found that the application of lower stocking densities lead to higher crop biomass. The high stocking density means, therefore, large amounts of metabolic waste, removed from the breeding units through their effluent, the recirculating flow of which is 4m3/h, enough to ensure the whole volume of water in a growth tank to be changed every half hour.

  11. Public, animal, and environmental health implications of aquaculture.

    Garrett, E. S.; C.L. dos Santos; Jahncke, M. L.

    1997-01-01

    Aquaculture is important to the United States and the world's fishery system. Both import and export markets for aquaculture products will expand and increase as research begins to remove physiologic and other animal husbandry barriers. Overfishing of wild stock will necessitate supplementation and replenishment through aquaculture. The aquaculture industry must have a better understanding of the impact of the "shrouded" public and animal health issues: technology ignorance, abuse, and neglec...

  12. Impact of water boundary layer diffusion on the nitrification rate of submerged biofilter elements from a recirculating aquaculture system

    Prehn, Jonas; Waul, Christopher Kevin; Pedersen, Lars-Flemming;

    2012-01-01

    Total ammonia nitrogen (TAN) removal by microbial nitrification is an essential process in recirculating aquaculture systems (RAS). In order to protect the aquatic environment and fish health, it is important to be able to predict the nitrification rates in RAS’s. The aim of this study was to...... determine the impact of hydraulic film diffusion on the nitrification rate in a submerged biofilter. Using an experimental batch reactor setup with recirculation, active nitrifying biofilter units from a RAS were exposed to a range of hydraulic flow velocities. Corresponding nitrification rates were...... measured following ammonium chloride, NH4Cl, spikes and the impact of hydraulic film diffusion was quantified. The nitrification performance of the tested biofilter could be significantly increased by increasing the hydraulic flow velocity in the filter. Area based first order nitrification rate constants...

  13. Automatic technology in a recirculating aquaculture system%工厂化水产养殖中的自动控制技术

    曹广斌; 蒋树义; 韩世成; 陈忠祥

    2011-01-01

    本文从工厂化水产养殖自动控制系统的组成、自动控制方法、自动控制技术在水质参数检测与调控中的应用等方面,论述了有关研究的技术成果和未来发展趋势.根据水产养殖工业化和自动化发展,以及自动控制技术在水产养殖中的广阔应用前景,指出了发展水产养殖自动化检测和调控精准技术的必要性,提出发展规范化、标准化的水产养殖自动化技术,以推动水产养殖工业化的发展.%The research and development of an automatic control system are summarized in the recirculating aquaculture, including the components of the system, the method of control, and the application of automatic technology in aquaculture.According to the need of aquaculture and the wide perspective application, it is necessary to research advanced automatic technology and to establish some standards for application of the technology in the recirculating aquaculture system.

  14. Electronic payment systems

    Malakjan, Stiv

    2010-01-01

    This thesis charts the evolution of Electronic payment systems and e-commerce. The goal is to create a complex picture about circumstances and causes that led to development and creation of new payment systems. The first part describes the evolution of money and payment systems that appeared during history. The second part focuses on e-money, electronic payment systems and the participants that are involved in payment processing trough this systems. It also explores the history of credit card...

  15. Analyzing and Evaluating of Recirculating Aquaculture Systems (RAS) of Rainbow Trout in Order to Designing a Conceptual Model of Efficient RAS in Iran (Tehran)

    M. Mahmoodzadeh; M Almassi; A.M. Borghei; B. Beheshti

    2013-01-01

    In this study, Recirculation Aquaculture Systems (RAS) of rainbow trout were analyzed and evaluated in Iran (Tehran). After analyzing these systems it was found which of them were better than others and then their parameters were used for designing a conceptual model of efficient RAS. This study was conducted in Iran (Tehran) in 2012 and statistical population and samples were 8 systems. Systems were analyzed and evaluated by five criteria which were as follows: economy, energy, consumption a...

  16. Aquaculture and food crisis: opportunities and constraints.

    Liao, I Chiu; Chao, Nai-Hsien

    2009-01-01

    Fish farming, now well known as aquaculture, has been well recognized since the ancient era. The first written document on fish culture was published in China in 475 BC, and the first koi pond was constructed at the Japanese Imperial Palace grounds during 71-130 AD. In recent years, aquaculture has progressively played an important role in the provision of: animal protein and gourmet cuisines, job opportunities, and foreign currency for developing countries. Asian countries produce around 91 percent of the world's total aquaculture production. Among the top ten aquaculture-producing countries, nine are from Asia. The current global population consist of more than 6.5 billion individuals; over one billion of which face hunger problem. In the highly populated Asia-Pacific region with moderately high-productivity, 642 million people are still facing hunger. Being a proficient and potential source of animal protein, aquaculture will play an increasing and important role in solving the world food problem in the future. This paper discusses both the opportunities and constraints in the aquaculture industry, specifically in the Asia-Pacific region, and its possible role in solving the current global food crisis. Strategies including promotion and adoption of traceability and HACCP systems for food safety, and marketing management for aquaculture products are also suggested. It is hoped that traditional administration of aquaculture management for survival, profit, as well as food safety will successfully match sustainability management to meet the urgent global need for food. PMID:19965349

  17. Electronic flight instrument system

    Hauptman, Luka

    2009-01-01

    This thesis describes basic concepts in research and development of a simple electronic flight instrument system, which displays piston engine data to the pilot. The main purpose is to build a functional prototype and acquire knowledge, which will enable us to further develop the system. The second chapter presents fundamentals of electronic flight instrument systems used in large commercial aircrafts. A detailed description of basic approaches to system implementation used by two of the b...

  18. Chemical composition and antimicrobial activity of the essential oil of Syzygium aromaticum flower bud (Clove) against fish systemic bacteria isolated from aquaculture sites

    Seongwei LEE; Musa NAJIAH; Wee WENDY; Musa NADIRAH

    2009-01-01

    This paper describes the chemical composition and antimicrobial activity of the essential oil of Syzygium aromaticum against Vibrio spp. (n=6), Edwardsiella spp. (n=21), Aeromonas spp. (n=2), Escherichia coli (n=2), Flavobacterium spp. (n=1), Salmonella spp. (n=2), Streptococcus spp. (n=1) and Pseudomonas spp. (n=1) isolated from aquaculture sites as well as seven reference strains of bacteria, namely, Escherichia coli (ATCC 25922), Citrobacter freundii (ATCC 8090), Aeromonas hydrophila (ATCC 49140), Pseudomonas aeruginosa (ATCC 35032), Streptococcus agalactiae (ATCC13813), Edwardsiella tarda (ATCC 15947) and Yersinia enterocolitica (ATCC 23715). Nowadays, most antibiotics are no longer effective in controlling diseases in aquaculture, especially fish systemic bacterial diseases, due to increasing incidences of antibiotic resistance among pathogenic bacteria. Furthermore, many countries have banned antibiotics in aquaculture use due to public health concerns and environmental hazards. Therefore, this study was carried out to evaluate the potential of the essential oil of S. aromaticum as an alternate commercial antibiotic to antimicrobial agents against fish systemic bacteria in aquaculture. The essential oil of S. aromaticum was prepared using a steam distillation method, and the chemical composition was analysed using Gas chromatography-mass spectroscopy (GC-MS). Minimum inhibitory concentration (MIC) values of the essential oils against the tested bacteria were determined using the broth two fold micro dilution method, with kanamycin and eugenol as positive controls. The MIC values of the essential oil of S. aromaticum ranged from 0.015 μg·mL-1 to 0.062 μg·mL-1 against the tested bacterial isolates. A total of nine chemical compounds were detected in the essential oil, with eugenol (49.0%) and caryophyllene (7.5%) being the major compounds. The results of the present study indicate that the essential oil of S. aromati- cum shows a huge potential to

  19. The Development of a Renewable-Energy-Driven Reverse Osmosis System for Water Desalination and Aquaculture Production

    Clark C K Liu

    2013-01-01

    Water and energy are closely linked natural resources-the transportation, treatment, and distribution of water depends on low-cost energy;while power generation requires large volumes of water. Seawater desalination is a mature technology for increasing freshwater supply, but it is essentially a trade of energy for freshwater and is not a viable solution for regions where both water and energy are in short supply. This paper discusses the development and application of a renewable-energy-driven reverse osmosis (RO) system for water desalination and the treatment and reuse of aquaculture wastewater. The system consists of (1) a wind-driven pumping subsystem, (2) a pressure-driven RO membrane desalination subsystem, and (3) a solar-driven feedback control module. The results of the pilot experiments indicated that the system, operated under wind speeds of 3 m s-1 or higher, can be used for brackish water desalination by reducing the salinity of feedwater with total dissolved solids (TDS) of over 3 000 mg L-1 to product water or permeate with a TDS of 200 mg L-1 or less. Results of the pilot experiments also indicated that the system can remove up to 97%of the nitrogenous wastes from the fish pond effluent and can recover and reuse up to 56%of the freshwater supply for fish pond operation.

  20. Molecular Analysis of Bacterial Communities and Detection of Potential Pathogens in a Recirculating Aquaculture System for Scophthalmus maximus and Solea senegalensis

    Patrícia Martins; Cleary, Daniel F. R.; Pires, Ana C. C.; Ana Maria Rodrigues; Victor Quintino; Ricardo Calado; Gomes, Newton C M

    2013-01-01

    The present study combined a DGGE and barcoded 16S rRNA pyrosequencing approach to assess bacterial composition in the water of a recirculating aquaculture system (RAS) with a shallow raceway system (SRS) for turbot (Scophthalmus maximus) and sole (Solea senegalensis). Barcoded pyrosequencing results were also used to determine the potential pathogen load in the RAS studied. Samples were collected from the water supply pipeline (Sup), fish production tanks (Pro), sedimentation filter (Sed), b...

  1. Hydrogen peroxide decomposition kinetics in aquaculture water

    Arvin, Erik; Pedersen, Lars-Flemming

    2015-01-01

    Hydrogen peroxide (HP) is used in aquaculture systems where preventive or curative water treatments occasionally are required. Use of chemical agents can be challenging in recirculating aquaculture systems (RAS) due to extended water retention time and because the agents must not damage the fish...... reared or the nitrifying bacteria in the biofilters at concentrations required to eliminating pathogens. This calls for quantitative insight into the fate of the disinfectant residuals during water treatment. This paper presents a kinetic model that describes the HP decomposition in aquaculture water...... application in RAS by addressing disinfection demand and identify efficient and safe water treatment routines....

  2. RESEARCHES REGARDING THE TECHNOLOGICAL PERFORMANCES OF CARP REARING DURING WINTER PERIOD IN THE CONDITIONS OF A RECIRCULATING AQUACULTURE SYSTEM

    G. STEFAN

    2013-12-01

    Full Text Available The techniques of carp culture are highly diversified, ranging from the extensive production in pond or open water with no fertilization or supplemental feeding to highly intensive systems in concrete tanks or cages. Among the different carp species, common carp is the best species reared in intensive monoculture, the others (Chinese and Indian carps being usually cultivated in polyculture (P. Kestemont, 1995. An experiment was conducted in inside recirculation system conditions to identify the technological performances on carp growth and survival at the Fishing and Aquaculture Department, Galati, during winter period (February, 2007 – March, 2007. The 1-year-old carp (Cyprinus carpio 4792g; 4594 g; 4561 g and 4525 g (total weight grew to 7384g; 7017g; 6924g and 7125 g in 44 days in aquarium 1, 2, 3 and 4, respectively. In all aquariums, the fish appeared healthy and no mortality was observed. Feed conversion efficiencies (FCE had similar values among all aquariums, the highest FCE being found in B4 aquarium with 1, 57 value. Water quality parameters were acceptable range for fish culture. Results show that the carp rearing during winter period in the inside recirculation system is a very good economic solution.

  3. Electronics circuits and systems

    Bishop, Owen

    2007-01-01

    The material in Electronics - Circuits and Systems is a truly up-to-date textbook, with coverage carefully matched to the electronics units of the 2007 BTEC National Engineering and the latest AS and A Level specifications in Electronics from AQA, OCR and WJEC. The material has been organized with a logical learning progression, making it ideal for a wide range of pre-degree courses in electronics. The approach is student-centred and includes: numerous examples and activities; web research topics; Self Test features, highlighted key facts, formulae and definitions. Each chapter ends with a set

  4. Electronics circuits and systems

    Bishop, Owen

    2011-01-01

    The material in Electronics - Circuits and Systems is a truly up-to-date textbook, with coverage carefully matched to the electronics units of the 2007 BTEC National Engineering and the latest AS and A Level specifications in Electronics from AQA, OCR and WJEC. The material has been organized with a logical learning progression, making it ideal for a wide range of pre-degree courses in electronics. The approach is student-centred and includes: numerous examples and activities; web research topics; Self Test features, highlighted key facts, formulae and definitions. Ea

  5. Disease in marine aquaculture

    Sindermann, C. J.

    1984-03-01

    populations. Some progress has been made in marine disease control through chemical treatment in intensive culture systems, principally through application and modification of methods developed for freshwater aquaculture. Major constraints to use of chemicals are restrictions due to public health concerns about food contamination, and the negative effects of some chemicals on biological filters and on algal food production. There is a continuing need, however, for development of specific treatments for acute disease episodes — such as the nitrofurans, developed in Japan, which are effective for some bacterial diseases. The history of aquaculture — freshwater as well as marine — has been characterized by transfers and introductions of species to waters beyond their present ranges. The process continues, and carries with it the possibility of transfers of pathogens to native species and to the recipient culture environments. International groups are attempting to define codes of practice to govern such mass movements, but examples of introductions of real or potential pathogens already exist. The most recent and the most dramatic is the world wide transfer of a virus pathogen of penaeid shrimps. Earlier examples include the introduction of a protozoan pathogen of salmonids to the western hemisphere, and the introduction of a parasitic copepod from the Far East to the west coast of North America and to France. The conclusion is inevitable — diseases are substantial deterrents to aquaculture production. Diagnostic and control procedures are and will be important components of emerging aquaculture technology.

  6. Effect of Hydraulic Loading Rate on the Efficiency of Effluent Treatment in a Recirculating Puffer Aquaculture System Coupled with Constructed Wetlands

    XU Jiabo; SHI Yonghai; ZHANG Genyu; LIU Jianzhong; ZHU Yazhu

    2014-01-01

    Constructed wetlands (CWs) were integrated into an indoor recirculating aquaculture system of obscure puffer (Takifugu obscurus) for effluent treatment. The effect of hydraulic loading rate (HLR) on the efficiency of effluent treatment by CWs was ex-amined for over a month. The CWs were operated under brackish conditions (salinity 7.4-7.6) at 3 different HLRs (0.762, 0.633, and 0.458 m d-1) 3 times, 10 days each. Overall, the CWs exhibited high efficiency in removal of total ammonium nitrogen (by 81.03-92.81%) and nitrite nitrogen (by 99.40%-99.68%). The efficiency of CWs in removal of total ammonium nitrogen, nitrate nitrogen, total Kjeldahl nitrogen, total phosphorous, and total suspended solids (TSS) increased with the decrease of HLR. The CWs operated at the 3 HLRs in a decreasing trend proves to be effective, providing a useful method for effluent treatment in commercial puffer aquaculture systems.

  7. Impact of water boundary layer diffusion on the nitrification rate of submerged biofilter elements from a recirculating aquaculture system.

    Prehn, Jonas; Waul, Christopher K; Pedersen, Lars-Flemming; Arvin, Erik

    2012-07-01

    Total ammonia nitrogen (TAN) removal by microbial nitrification is an essential process in recirculating aquaculture systems (RAS). In order to protect the aquatic environment and fish health, it is important to be able to predict the nitrification rates in RAS's. The aim of this study was to determine the impact of hydraulic film diffusion on the nitrification rate in a submerged biofilter. Using an experimental batch reactor setup with recirculation, active nitrifying biofilter units from a RAS were exposed to a range of hydraulic flow velocities. Corresponding nitrification rates were measured following ammonium chloride, NH₄Cl, spikes and the impact of hydraulic film diffusion was quantified. The nitrification performance of the tested biofilter could be significantly increased by increasing the hydraulic flow velocity in the filter. Area based first order nitrification rate constants ranged from 0.065 m d⁻¹ to 0.192 m d⁻¹ for flow velocities between 2.5 m h⁻¹ and 40 m h⁻¹ (18 °C). This study documents that hydraulic film diffusion may have a significant impact on the nitrification rate in fixed film biofilters with geometry and hydraulic flows corresponding to our experimental RAS biofilters. The results may thus have practical implications in relation to the design, operational strategy of RAS biofilters and how to optimize TAN removal in fixed film biofilter systems. PMID:22507250

  8. Ammonia-oxidizing archaea and nitrite-oxidizing nitrospiras in the biofilter of a shrimp recirculating aquaculture system.

    Brown, Monisha N; Briones, Aurelio; Diana, James; Raskin, Lutgarde

    2013-01-01

    This study analysed the nitrifier community in the biofilter of a zero discharge, recirculating aquaculture system (RAS) for the production of marine shrimp in a low density (low ammonium production) system. The ammonia-oxidizing populations were examined by targeting 16S rRNA and amoA genes of ammonia-oxidizing bacteria (AOB) and archaea (AOA). The nitrite-oxidizing bacteria (NOB) were investigated by targeting the 16S rRNA gene. Archaeal amoA genes were more abundant in all compartments of the RAS than bacterial amoA genes. Analysis of bacterial and archaeal amoA gene sequences revealed that most ammonia oxidizers were related to Nitrosomonas marina and Nitrosopumilus maritimus. The NOB detected were related to Nitrospira marina and Nitrospira moscoviensis, and Nitrospira marina-type NOB were more abundant than N. moscoviensis-type NOB. Water quality and biofilm attachment media played a role in the competitiveness of AOA over AOB and Nitrospira marina-over N. moscoviensis-type NOB. PMID:22775980

  9. Effect of Recirculating Aquaculture System (RAS) on Growth Performance, Body Composition and Hematological Indicators of Allogynogenetic crucian Carp (Carassius auratus gibelio)

    Xiao-li Li; Gu Li; Shi-yang Zhang; Ling Tao

    2013-01-01

    A Ditch Constructed Wetland unit (DCW) was integrated into an outdoor RAS with four fishponds. This study evaluated the performance of the wetland unit in treating the recirculating wastewater and examined the effect of Recirculating Aquaculture System (RAS) on growth performance, body composition and hematological indicators of the rearing fish. During a 165-days culture period, the DCW effectively reduced the influent concentrations of nutrients and can keep a good water quality at acceptab...

  10. Leaf vegetables for use in integrated hydroponics and aquaculture systems: Effects of root flooding on growth, mineral composition and nutrient uptake

    Trang, Ngo Thuy Diem; Schierup, Hans-Henrik; Brix, Hans

    2010-01-01

    In recirculating aquaculture and hydroponics systems, the waste products from fish production are used to produce vegetables or other crops of economic value, and the water is recirculated to the fish tanks. We studied growth, productivity and nutrient uptake of four leaf vegetable species (Lactuca sativa, Ipomoea aquatica, Brassica rapa var. chinensis and Brassica rapa var. parachinensis) in a controlled growth experiment with three root flooding treatments (drained, half-flooded and flooded...

  11. Isotopes in aquaculture research

    The applications of isotopes in aquaculture research include areas like aquatic production process, nutrient cycles and food chain dynamics, fish nutrition, fish physiology, genetics and immunology. The radioisotopes commonly used are beta emitters. The use of different radioisotopes in aquaculture research are presented. 2 tabs

  12. Single-sludge denitrification in recirculating aquaculture systems: Effects of pre-fermentation and pH

    Suhr, Karin Isabel; Letelier Gordo, Carlos Octavio; Prat Busquets, Pau

    2014-01-01

    Single-sludge denitrification (DN) reactors in aquaculture utilize the solid fish waste produced in the system to reduce the nitrate load discharged. The solid waste is available for denitrifiers when present in soluble readily biodegradable form. A transformation accomplished by bacterial...... hydrolysis (and fermentation). The objective of this study was to quantify the effect of pre-fermentation of the solid fish waste on single-sludge DN-reactor efficiency. Pre-fermentation times tested were; 0 (no pre-fermentation), 1 d, 5 d, and 10 d. The efficiency was quantified as the potential DN-rates in...... laboratory assessments. Results showed that the highest DN-rate obtained was achieved by 1 d pre-fermentation. The volumetric DN-rate measured in decreasing order was; 23.4 ± 0.00 (1 d); 20.5 ± 0.35 (5 d); 17.0 ± 0.47 (10 d); 14.2 ± 0.24 (0 d) mg NO3-N (L∗h)-1 It was suspected that the poor utilization of s...

  13. Single-sludge denitrification in recirculating aquaculture systems: effects of pre-fermentation and pH

    Suhr, Karin Isabel; Letelier-Gordo, Carlos Octavio; Prat Busquets, Pau

    2015-01-01

    Single-sludge denitrification (DN) reactors in aquaculture use the carbonous solid fish waste produced in the system to reduce the discharged nitrate load. The solid waste is available for denitrifiers when present in soluble, readily biodegradable form, and the transformation is accomplished by...... bacterial hydrolysis and fermentation. The objective of this study was to quantify the effect of pre-fermentation of solid fish waste on single-sludge DN reactor efficiency. Pre-fermentation times tested were 0 d (no pre-fermentation), 1 d, 5 d, and 10 d, and the efficiency was quantified as the potential...... 14.2 +/- 0.24 mg NO3-N L-1 h(-1) (0 d). It was suspected that the poor utilization of soluble COD (sCOD) in the 5 d and 10 d pre-fermentation treatments was due to the low starting pH (pH <7). Subsequently, the experiments were repeated in 0.1 M HEPES buffer (pH = 7.1) and showed a clear correlation...

  14. Biological denitrification using poly(butylene succinate) as carbon source and biofilm carrier for recirculating aquaculture system effluent treatment.

    Zhu, Song-Ming; Deng, Ya-Le; Ruan, Yun-Jie; Guo, Xi-Shan; Shi, Ming-Ming; Shen, Jia-Zheng

    2015-09-01

    Nitrate removal is essential for the sustainable operation of recirculating aquaculture system (RAS). This study evaluated the heterotrophic denitrification using poly(butylene succinate) as carbon source and biofilm carrier for RAS wastewater treatment. The effect of varied operational conditions (influent type, salinity and nitrate loading) on reactor performance and microbial community was investigated. The high denitrification rates of 0.53 ± 0.19 kg NO3(-)-N m(-3) d(-1) (salinity, 0‰) and 0.66 ± 0.12 kg NO3(-)-Nm(-3) d(-1) (salinity, 25‰) were achieved, and nitrite concentration was maintained below 1mg/L. In addition, the existence of salinity exhibited more stable nitrate removal efficiency, but caused adverse effects such as excessive effluent dissolved organic carbon (DOC) and dissimilation nitrate reduce to ammonia (DNRA) activity. The degradation of PBS was further confirmed by SEM and FTIR analysis. Illumina sequencing revealed the abundance and species changes of functional denitrification and degradation microflora which might be the primary cause of varied reactor performance. PMID:26093254

  15. The effects of different seeding ratios on nitrification performance and biofilm formation in marine recirculating aquaculture system biofilter.

    Zhu, Songming; Shen, Jiazheng; Ruan, Yunjie; Guo, Xishan; Ye, Zhangying; Deng, Yale; Shi, Mingming

    2016-07-01

    Rapid start-up of biofilter is essential for intensive marine recirculating aquaculture system (RAS) production. This study evaluated the nitrifying biofilm formation using mature biofilm as an inoculum to accelerate the process in RAS practice. The effects of inoculation ratios (0-15 %) on the reactor performance and biofilm structure were investigated. Complete nitrification was achieved rapidly in reactors with inoculated mature biofilm (even in 32 days when 15 % seeding ratio was applied). However, the growth of target biofilm on blank carrier was affected by the mature biofilm inoculated through substrate competition. The analysis of extracellular polymeric substance (EPS) and nitrification rates confirmed the divergence of biofilm cultivation among reactors. Besides, three N-acyl-homoserine lactones (AHLs) were found in the process, which might regulate the activities of biofilm. Multivariate analysis based on non-metric multidimensional scaling (nMDS) also indicated the great roles of AHLs and substrate supply which might fundamentally determine varied cultivation performance on target biofilm. PMID:27068911

  16. The Effects of Some Phytobiotics on Biochemical Composition of Oreochromis Niloticus Meat Reared in a Recirculating Aquaculture System

    Alina Antache

    2013-05-01

    Full Text Available The purpose of this experiment is the evaluation of biochemical composition of Nile tilapia meat by administrating  several phytobiotics in feed. Oreochromis niloticus, with an initial average weight of 125.41±34.33 g/exemplar, were reared, during six weeks in a recirculating aquaculture system. The phytobiotics from this experiment were administered in feed in a concentration of 1%/kg feed and consist in: thyme (Thymus vulgaris, fenugreek (Trigonela foenum graecum, neem (Azadirachta indica. Thus, the experimental variants were: V1-control, V2-thyme, V3-fenugreek and V4-neem. Fish were fed with SOPROFISH pelleted feed with 38% crude protein and 7% crude fat. During the experiment, the physico-chemical parameters of technological water were situated in normal range for optimal growth. At the end of the experiment, significant differences (p<0.05 were recorded between the experimental variants, regarding to moisture, protein content, fat content and dry matter; unsignificant differences were recorded regarding the percentage of ash (p>0.05; p=0.68. The highest value of moisture (87.25% was registered in V2; the lowest value of fat content (0.05% was recorded in V4 and the lowest protein content (10.79% in V2. In conclusion, thyme, fenugreek and neem administration, in concentration of 1%/kg feed, influenced significantly the biochemical composition of Oreochromis niloticus.

  17. Application of CFD Modeling to Hydrodynamics of CycloBio Fluidized Sand Bed in Recirculating Aquaculture Systems

    LIU Yao; SONG Xiefa; LIANG Zhenlin; PENG Lei

    2014-01-01

    To improve the efficiency of a CycloBio fluidized sand bed (CB FSB) in removal of dissolved wastes in recirculating aquaculture systems, the hydrodynamics of solid-liquid flow was investigated using computational fluid dynamics (CFD) modeling tools. The dynamic characteristics of silica sand within the CB FSB were determined using three-dimensional, unsteady-state simula-tions with the granular Eulerian multiphase approach and the RNG k-ε turbulence model, and the simulation results were validated using available lab-scale measurements. The bed expansion of CB FSB increased with the increase in water inflow rate in numerical simulations. Upon validation, the simulation involving 0.55 mm particles, the Gidaspow correlation for drag coefficient model and the Syamlal-O’Brien correlation for kinetic granular viscosity showed the closest match to the experimental results. The volume frac-tion of numerical simulations peaked as the wall was approached. The hydrodynamics of a pilot-scale CB FSB was simulated in or-der to predict the range of water flow to avoid the silica sand overflowing. The numerical simulations were in agreement with the experimental results qualitatively and quantitatively, and thus can be used to study the hydrodynamics of solid-liquid multiphase flow in CB FSB, which is of importance to the design, optimization, and amplification of CB FSBs.

  18. Effects of solid-phase denitrification on the nitrate removal and bacterial community structure in recirculating aquaculture system.

    Qiu, Tianlei; Liu, Lili; Gao, Min; Zhang, Lanhe; Tursun, Haireti; Wang, Xuming

    2016-06-01

    A solid-phase denitrification (SPD) reactor packed with poly (3-hydroxybutyrate-co-3-hydroxyvalerate) as a carbon source was incorporated into a recirculating aquaculture system (RAS) to remove accumulated nitrate. Bacterial community structures in different parts of the RAS, including biofilter unit, SPD reactor, and culture water, were analyzed using Illumina MiSeq sequencing technology. The data showed that nitrate levels decreased remarkably in the RAS connected with SPD reactor (RAS-DR). In contrast, nitrate levels increased continuously in the conventional RAS without SPD reactor (RAS-CK). Biofilter unit and culture water in RAS-DR developed lower species richness and higher bacterial community diversity than that in RAS-CK. The bacterial community structure of RAS was significantly affected by the SPD process and the changes included an increase in the proportion of Proteobacteria and Firmicutes and a decrease in Nitrospira abundance in RAS-DR. Firmicutes was the most abundant phylum (56.9 %) and mainly consisted of Clostridium sensu stricto (48.3 %) in SPD reactor. PMID:27125529

  19. The Relationship between the Area of Aquaculture Pond and Purification Pond in Water Circulation Aquaculture System%池塘循环水养殖模式下养殖面积与净化面积的配比关系研究

    宋超; 陈家长; 戈贤平; 孟顺龙; 范立民; 胡庚东

    2013-01-01

    The establishment of water circulation aquaculture system realized the hierarchical use of nitrogen,phosphorus and other eutrophic substances in aquaculture wastewater and the recirculation use of water resource.However,no research has been reported on the detailed calculation of the relationship between the area of aquaculture pond and purification pond.In this study,referring to the absorption ability of aquatic plants to pollutants in aquaculture wastewater and pollutant generation and discharge coefficient in aquaculture pond,based on the general rules of water quality management in freshwater aquaculture system,a calculation mode was established to investigate the relationship between the area of aquaculture pond and purification pond in freshwater recirculation aquaculture system,which was feasible to explain related cases and would provide theoretical basis to reduce the economic costs in the construction of water circulation aquaculture system and realize the balance between the ecological benefits and the economic benefits.%池塘循环水养殖模式的构建实现了养殖废水中氮、磷等富营养化物质的分级利用和水资源的循环使用,但此前的研究并没有详细计算养殖面积和净化面积合理的配比关系.通过参照水生植物对养殖尾水中污染物的吸收能力和养殖鱼类的产排污系数,再结合淡水池塘养殖过程中的水质管理的一般规律,给出了淡水池塘循环水养殖模式中养殖池塘面积和净化池塘面积之间配比关系的计算方法.利用该计算方法来解释此前相关的研究实例,也证明是可行的.该计算模型的构建为今后在池塘循环水养殖模式构建中降低经济成本,为最终实现生态效益对经济效益的补偿提供了理论基础.

  20. Leaf vegetables for use in integrated hydroponics and aquaculture systems: Effects of root flooding on growth, mineral composition and nutrient uptake

    Trang, Ngo Thuy Diem; Schierup, Hans-Henrik; Brix, Hans

    2010-01-01

    In recirculating aquaculture and hydroponics systems, the waste products from fish production are used to produce vegetables or other crops of economic value, and the water is recirculated to the fish tanks. We studied growth, productivity and nutrient uptake of four leaf vegetable species (Lactuca...... sativa, Ipomoea aquatica, Brassica rapa var. chinensis and Brassica rapa var. parachinensis) in a controlled growth experiment with three root flooding treatments (drained, half-flooded and flooded) to assess their preferred hydroponic growth requirements, biomass production and nutrient removal......, respectively. The two Brassica varieties produced much less aerial biomass (50-54 g DW/m2 during a 60-day period). Both I. aquatica and L. sativa are promising species to be included in integrated hydroponic and aquaculture facilities, with I. aquatica showing the most promise because of its higher growth...

  1. Electronic components and systems

    Dennis, W H

    2013-01-01

    Electronic Components and Systems focuses on the principles and processes in the field of electronics and the integrated circuit. Covered in the book are basic aspects and physical fundamentals; different types of materials involved in the field; and passive and active electronic components such as capacitors, inductors, diodes, and transistors. Also covered in the book are topics such as the fabrication of semiconductors and integrated circuits; analog circuitry; digital logic technology; and microprocessors. The monograph is recommended for beginning electrical engineers who would like to kn

  2. Importance of kelp-derived organic carbon to the scallop Chlamys farreri in an integrated multi-trophic aquaculture system

    Xu, Qiang; Gao, Fei; Yang, Hongsheng

    2016-03-01

    Bivalves and seaweeds are important cleaners that are widely used in integrated multi-trophic aquaculture (IMTA) systems. A beneficial relationship between seaweed and bivalve in the seaweed-based IMTA system has been confirmed, but the trophic importance of seaweed-derived particulate organic materials to the co-cultured bivalve is still unclear. We evaluated the trophic importance of the kelp Saccharina japonica to the co-cultured scallop Chlamys farreri in a typical IMTA farm in Sungo Bay (Weihai, North China). The dynamics of detritus carbon in the water were monitored during the culturing period. The proportion of kelp-derived organic matter in the diet of the co-cultured scallop was assessed via the stable carbon isotope method. Results showed that the detritus carbon in the water ranged from 75.52 to 265.19 μg/L, which was 25.6% to 73.8% of total particulate organic carbon (TPOC) during the study period. The amount of detritus carbon and its proportion in the TPOC changed throughout the culture cycle of the kelp. Stable carbon isotope analysis showed that the cultured scallop obtained 14.1% to 42.8% of its tissue carbon from the kelp, and that the percentages were closely correlated with the proportion of detritus carbon in the water ( F =0.993, P= 0.003). Evaluation showed that for 17 000 tons (wet weight) of annual scallop production, the kelp contributed about 139.3 tons of carbon (535.8 tons of dry mass). This confirms that cultured kelp plays a similar trophic role in IMTA systems as it does in a natural kelp bed. It is a major contributor to the detritus pool and supplies a vital food source to filter-feeding scallops in the IMTA system, especially during winter and early spring when phytoplankton are scarce.

  3. Nitrogen and Phosphorus Removal in the Recirculating Aquaculture System with Water Treatment Tank containing Baked Clay Beads and Chinese Cabbage

    Aeknarin Thanakitpairin

    2014-01-01

    Full Text Available This research aims to describe the nitrogen and phosphorus removal in Recirculating Aquaculture System (RAS by crop plants biomass production. The 3 experiment systems consisted of 1 treatment (fish tank + baked clay beads + Chinese cabbage and 2 controls as control-1 (fish tank only and control-2 (fish tank + baked clay beads, were performed. With all experimental RAS, Nile tilapia (Oreochromis niloticus was cultured at 2 kg/m3 density. The baked clay beads (8-16 mm in diameter were filled as a layer of 10 cm in the water treatment tank of control-2. While in the treatment tank, Chinese cabbage (Brassica pekinensis was planted at 334 plants/m2 in baked clay beads layer. During 35 days of experiment, the average fish wet-weight in control-1, control-2 and treatment systems increased from 16.31±1.49, 15.18±1.28 and 11.31±1.49 g to 29.43±7.06, 28.65±3.12 and 27.20±6.56 g, respectively. It was found that the growth rate of 0.45±0.15 g-wet weight/day in a treatment tank was higher than in those 2 controls, which were rather similar at 0.37±0.16 and 0.38±0.05 g-wet weight/day, respectively. The fish survival rate of all experimental units was 100%. The average Chinese cabbage wet-weight in treatment system increased from 0.15±0.02 g to 1.00±0.38 g. For water quality, all parameters were within the acceptable range for aquaculture. The assimilation inorganic nitrogen in a treatment tank showed a slower rate and lower nitrite accumulation relative to those in control tanks. The nitrogen and phosphorus balance analysis illustrated that most of the nitrogen and phosphorus input in all systems was from feed (82-87% and 21-87% while at the final day of experiments, nitrogen and phosphorus in tilapia culture revealed at 15-19% and 4-13%. The accumulation of nitrogen and phosphorus in the water, up to 56% and 70%, was found in control-1 while water in the tank with baked clay beads had substantial lower nitrogen and phosphorus concentration. The

  4. Use of Probiotics in Aquaculture

    2002-01-01

    Diseases of cultured animal and environmental pollution are very important problems in aquaculture.The use of probiotics in aquaculture has more adva ntages than the use of antibiotics and chemicals in aquaculture. This review int roduces the use and mechanisms of the probiotics in aquaculture.

  5. Performance of Nile tilapia Oreochromis niloticus fingerlings in a hyper-intensive recirculating aquaculture system with low water exchange

    M Gullian-Klanian

    2013-03-01

    Full Text Available The aim of this paper was evaluate the performance of Nile tilapia fingerlings (Oreochromis niloticus raised at hyper intensive stocking density in a recirculating aquaculture system (RAS with minimum water replacing. The experimental system was performed in a single-batch nursery system to obtain 50 g fish size in 60 days. Fish (2.07 ± 0.04 g were stocked in triplicate at 400 (T1, 500 (T2 and 600 (T3 fish m-3 (0.84, 1.05, 1.22 kg m-3. RAS functioned with 12,000 L of recirculating water and 252 L day-1of water replacing (2.1% daily. The efficiency of the biofilter for removing the total ammonia nitrogen (TAN was 48 ± 12.5 mg L-1. Stocking density did not affect significantly the survival (89.5 to 93.6%. The growth rate of T1 (0.96 g day-1; 5.01% day-1 and T2 (0.92 g day-1; 4.95 % day-1 was significantly higher than T3 (0.83 g day-1; 4.80% day-1. The specific growth rate (SGR of T1 was 41% influenced by temperature. For T2 and T3 the SGR were influenced by the variation of dissolved oxygen (DO that explained 47 and 44% of the fish weight variation, respectively. The SGR from T3 was also affected by the concentration of ammonia nitrogen (31%. The high stocking density affected the overall size of fish and the size homogeneity, but had no negative effect on the length-weight relationship (L-W. Data support the conclusion that fingerling stocked at 400 and 500 fish m-3 shows high performance during 9-weeks when the biomass not exceed 37 kg m-3. At this time fish have reached the desired final nursery weight (50 g for transfer to grow-out facilities.

  6. 臭氧处理技术在工厂化水产养殖中的应用研究%Application of Ozone in Recirculating Aquaculture System

    周煊亦; 房燕; 曹广斌; 蒋树义; 韩世成; 陈忠祥; 郭常有

    2012-01-01

    臭氧消毒是工厂化水产养殖中水处理的关键技术之一。本文详细介绍了臭氧的物理、化学性质,论述了臭氧制造、水中溶解及水中溶解浓度的检测方法。结合国内外有关水产养殖水处理技术的研究成果与发展趋势,分析了臭氧在工厂化水产养殖水处理中的一些作用和应用特性。介绍了臭氧在工厂化水产养殖中消毒杀菌、氧化有机物、凝聚悬浮物、除臭与除色方面的作用,阐明了臭氧在工厂化水产养殖中的应用前景。%Ozone disinfection of water is one kind of key technology in water treatment of recirculating aquaculture system.This paper describes the physical and chemical nature of ozone and several ways of manufacturing,dissolving and detection of ozone.Based on aquaculture water treatment technology research and development trends,some of the role and the application feature that ozone used in aquaculture water treatment factory are analyzed.In recirculating aquaculture,ozone can be used in disinfection and sterilization,oxidation of organic compounds,suspended solids cohesion,deodorization and decolourization.Meanwhile,the application prospect of ozone was discussed.

  7. Nitrogen removal techniques in aquaculture for a sustainable production

    Crab, R.; Avnimelech, Y.; Defoirdt, T.; Bossier, P.; Verstraete, W.

    2007-01-01

    As the aquaculture industry intensively develops, its environmental impact increases. Discharges from aquaculture deteriorate the receiving environment and the need for fishmeal and fish oil for fish feed production increases. Rotating biological contactors, trickling filters, bead filters and fluidized sand biofilters are conventionally used in intensive aquaculture systems to remove nitrogen from culture water. Besides these conventional water treatment systems, there are other possible mod...

  8. Electron beam processing system

    Electron beam Processing Systems (EPS) are used as useful and powerful tools in many industrial application fields such as the production of cross-linked wire, rubber tire, heat shrinkable film and tubing, curing, degradation of polymers, sterilization and environmental application. In this paper, the feature and application fields, the selection of machine ratings and safety measures of EPS will be described. (author)

  9. Uncultured bacterial diversity in a seawater recirculating aquaculture system revealed by 16S rRNA gene amplicon sequencing.

    Lee, Da-Eun; Lee, Jinhwan; Kim, Young-Mog; Myeong, Jeong-In; Kim, Kyoung-Ho

    2016-04-01

    Bacterial diversity in a seawater recirculating aquaculture system (RAS) was investigated using 16S rRNA amplicon sequencing to understand the roles of bacterial communities in the system. The RAS was operated at nine different combinations of temperature (15°C, 20°C, and 25°C) and salinity (20‰, 25‰, and 32.5‰). Samples were collected from five or six RAS tanks (biofilters) for each condition. Fifty samples were analyzed. Proteobacteria and Bacteroidetes were most common (sum of both phyla: 67.2% to 99.4%) and were inversely proportional to each other. Bacteria that were present at an average of ≥ 1% included Actinobacteria (2.9%) Planctomycetes (2.0%), Nitrospirae (1.5%), and Acidobacteria (1.0%); they were preferentially present in packed bed biofilters, mesh biofilters, and maturation biofilters. The three biofilters showed higher diversity than other RAS tanks (aerated biofilters, floating bed biofilters, and fish tanks) from phylum to operational taxonomic unit (OTU) level. Samples were clustered into several groups based on the bacterial communities. Major taxonomic groups related to family Rhodobacteraceae and Flavobacteriaceae were distributed widely in the samples. Several taxonomic groups like [Saprospiraceae], Cytophagaceae, Octadecabacter, and Marivita showed a cluster-oriented distribution. Phaeobacter and Sediminicola-related reads were detected frequently and abundantly at low temperature. Nitrifying bacteria were detected frequently and abundantly in the three biofilters. Phylogenetic analysis of the nitrifying bacteria showed several similar OTUs were observed widely through the biofilters. The diverse bacterial communities and the minor taxonomic groups, except for Proteobacteria and Bacteroidetes, seemed to play important roles and seemed necessary for nitrifying activity in the RAS, especially in packed bed biofilters, mesh biofilters, and maturation biofilters. PMID:27033205

  10. Optimization and evaluation of a bottom substrate denitrification tank for nitrate removal from a recirculating aquaculture system.

    Pungrasmi, Wiboonluk; Playchoom, Cholticha; Powtongsook, Sorawit

    2013-08-01

    A bottom substrate denitrification tank for a recirculating aquaculture system was developed. The laboratory scale denitrification tank was an 8 L tank (0.04 m2 tank surface area), packed to a depth of 5 cm with a bottom substrate for natural denitrifying bacteria. An aquarium pump was used for gentle water mixing in the tank; the dissolved oxygen in the water was maintained in aerobic conditions (e.g. > 2 mg/L) while anoxic conditions predominated only at the bottom substrate layer. The results showed that, among the four substrates tested (soil, sand, pumice stone and vermiculite), pumice was the most preferable material. Comparing carbon supplementation using methanol and molasses, methanol was chosen as the carbon source because it provided a higher denitrification rate than molasses. When methanol was applied at the optimal COD:N ratio of 5:1, a nitrate removal rate of 4591 +/- 133 mg-N/m2 tank bottom area/day was achieved. Finally, nitrate removal using an 80 L denitrification tank was evaluated with a 610 L recirculating tilapia culture system. Nitrate treatment was performed by batch transferring high nitrate water from the nitrification tank into the denitrification tank and mixing with methanol at a COD:N ratio of 5:1. The results from five batches of nitrate treatment revealed that nitrate was successfully removed from water without the accumulation of nitrite and ammonia. The average nitrate removal efficiency was 85.17% and the average denitrification rate of the denitrification tank was 6311 +/- 945 mg-N/m2 tank bottom area/day or 126 +/- 18 mg-N/L of pumice packing volume/day. PMID:24520693

  11. Optimization and evaluation of a bottom substrate denitrification tank for nitrate removal from a recirculating aquaculture system

    Wiboonluk Pungrasmi; Cholticha Playchoom; Sorawit Powtongsook

    2013-01-01

    A bottom substrate denitrification tank for a recirculating aquaculture system was developed.The laboratory scale denitrification tank was an 8 L tank (0.04 m2 tank surface area),packed to a depth of 5 cm with a bottom substrate for natural denitrifying bacteria.An aquarium pump was used for gentle water mixing in the tank; the dissolved oxygen in the water was maintained in aerobic conditions (e.g.> 2 mg/L) while anoxic conditions predominated only at the bottom substrate layer.The results showed that,among the four substrates tested (soil,sand,pumice stone and vermiculite),pumice was the most preferable material.Comparing carbon supplementation using methanol and molasses,methanol was chosen as the carbon source because it provided a higher denitrification rate than molasses.When methanol was applied at the optimal COD∶N ratio of 5∶1,a nitrate removal rate of 4591 ± 133 mg-N/m2 tank bottom area/day was achieved.Finally,nitrate removal using an 80 L denitrification tank was evaluated with a 610 L recirculating tilapia culture system.Nitrate treatment was performed by batch transferring high nitrate water from the nitrification tank into the denitrification tank and mixing with methanol at a COD∶N ratio of 5∶1.The results from five batches of nitrate treatment revealed that nitrate was successfully removed from water without the accumulation of nitrite and ammonia.The average nitrate removal efficiency was 85.17% and the average denitrification rate of the denitrification tank was 6311 ± 945 mg-N/m2 tank bottom area/day or 126 ± 18 mg-N/L of pumice packing volume/day.

  12. Environmental constraints and aquaculture development

    Paquotte, Philippe; Bailly, Denis

    1994-01-01

    Aquaculture is an economic activit y which is characterised by a great dependence on environmental conditions. Therefore, aquaculture development is subdued to institutional context which defines the rules for use and conservation of natural resources. This paper analyses the impact of marine environment regulations on economic operating of aquaculture enterprises and on industrial d ynamics. Because aquaculture enterprises use common goods, an economic approach of aquaculture has to take int...

  13. DNA vaccines for aquacultured fish

    Lorenzen, Niels; LaPatra, S.E.

    2005-01-01

    fully addressed, although inherently the risks should not be any greater than with the commercial fish vaccines that are currently used. Present classification systems lack clarity in distinguishing DNA-vaccinated animals from genetically modified organisms (GMOs), which could raise issues in terms of...... licensing and public acceptance of the technology. The potential benefits of DNA vaccines for farmed fish include improved animal welfare, reduced environmental impacts of aquaculture activities, increased food quality and quantity, and more sustainable production. Testing under commercial production...

  14. Aquaculture in mangrove environment

    Parulekar, A.H.

    of prawns, bivalves and finfishes. General consensus based on the analysis of cost factor, pond management efficiency and annual production levels has indicated that the disadvantages of setting up of large-scale commercial aquaculture ventures in mangrove...

  15. Microalgae for aquaculture

    Michels, M.H.A.

    2015-01-01

    In 2007, the project ‘Zeeuwse Tong’ (Zeeland Sole) was founded with support of the province of Zeeland, the Netherlands. The aim of the Zeeuwse Tong project was to establish an innovative land-based integrated multi-trophic aquaculture sector, which is producing sole, ragworms, algae, shellfish and saline crops in close harmony with nature. The project was divided into two sub-projects: The integrated saline aquaculture farm and the integrated nursery. The research described in this thesis re...

  16. Zebrafish as animal model for aquaculture nutrition research

    Ulloa, Pilar E.; Medrano, Juan F.; Feijoo, Carmen G.

    2014-01-01

    The aquaculture industry continues to promote the diversification of ingredients used in aquafeed in order to achieve a more sustainable aquaculture production system. The evaluation of large numbers of diets in aquaculture species is costly and requires time-consuming trials in some species. In contrast, zebrafish (Danio rerio) can solve these drawbacks as an experimental model, and represents an ideal organism to carry out preliminary evaluation of diets. In addition, zebrafish has a sequen...

  17. Association of mycobacteria in recirculating aquaculture systems and mycobacterial disease in fish.

    Yanong, Roy P E; Pouder, Deborah B; Falkinham, Joseph O

    2010-12-01

    Mycobacterium marinum isolates cultivated from tissue containing granulomatous lesions in Florida pompano Trachinotus carolinus and from biofilm samples collected from their tank and water recirculating system had identical (L1 of 11 bands) repetitive-sequence-based polymerase chain reaction (rep-PCR) DNA fingerprints. A second M. marinum clone sharing 4 of 11 rep-PCR bands with the first clone was isolated from some fish tissues but not from system samples. Water samples yielded low numbers of colonies of mycobacteria (0.08-1.3/mL), but high numbers were recovered from biofilms (260-12,000/swab) and filters (63-21,000/ filter). Mycobacterium hemophilum, M. chelonae, M. trivale, M. gastri, and M. gordonae were isolated from system samples alone. PMID:21413504

  18. Cooling system for electronic components

    Anderl, William James; Colgan, Evan George; Gerken, James Dorance; Marroquin, Christopher Michael; Tian, Shurong

    2015-12-15

    Embodiments of the present invention provide for non interruptive fluid cooling of an electronic enclosure. One or more electronic component packages may be removable from a circuit card having a fluid flow system. When installed, the electronic component packages are coincident to and in a thermal relationship with the fluid flow system. If a particular electronic component package becomes non-functional, it may be removed from the electronic enclosure without affecting either the fluid flow system or other neighboring electronic component packages.

  19. Cooling system for electronic components

    Anderl, William James; Colgan, Evan George; Gerken, James Dorance; Marroquin, Christopher Michael; Tian, Shurong

    2016-05-17

    Embodiments of the present invention provide for non interruptive fluid cooling of an electronic enclosure. One or more electronic component packages may be removable from a circuit card having a fluid flow system. When installed, the electronic component packages are coincident to and in a thermal relationship with the fluid flow system. If a particular electronic component package becomes non-functional, it may be removed from the electronic enclosure without affecting either the fluid flow system or other neighboring electronic component packages.

  20. Performance of an in-situ rotating biological contactor in a recirculating aquaculture system.

    Marin, P; Donoso-Bravo, A; Campos, J L; Ruiz-Filippi, G; Chamy, R

    2011-01-01

    The start-up and activation of a nitrifying rotating biological contactor (RBC) and its performance inside a culture tank of rainbow trout were studied. First, in a lab-scale operation, the system was fed with a synthetic medium containing a high ammonia concentration (567 mg NH(4)(+)-N L(-1)) and operated at a high hydraulic retention time (HRT) (6.5 days) to minimize the wash-out of the biomass and promote the biofilm formation. Then, both inlet ammonia concentration and HRT were decreased in order to obtain operational conditions similar to those of the culture tank. During this period, the RBC was able to treat an ammonia loading rate (ALR) of 0.64 g N-NH(4)(+) L(-1) d(-1) with a removal efficiency within 70-100%. Pilot-scale experiments were carried out in culture tanks of rainbow trout. The operation of a recirculating system with the RBC unit was compared with a recirculating system without biological treatment and with a flow-through system. The use of this in-situ nitrifying unit allowed working at a recirculation ratio of 90% without negative effects on either growth or the condition factor of fishes. Up to 70% of ammonia generated was removed and a removal rate of 1.41 g NH(4)(+)-N m(-2) d(-1) was reached. PMID:22156125

  1. Capacity building improve Malaysia's inspection and monitoring system for aquaculture and fishery products

    Gevers, G.J.M.; Zoontjes, P.W.; Essers, M.L.; Klijnstra, M.; Gerssen, A.

    2012-01-01

    The project aimed to help build a credible inspection and monitoring system that can guarantee safe quality products of Ministry of Health (MoH) and Department of Fisheries (DoF) by upgrading the analytical capacity of the laboratory staff directly involved in the analysis and detection of forbidden

  2. Preliminary research on the anammox process and control of nitrogen compounds in a recirculating aquaculture system

    Savin Cristian; Păsărin Benone; Patriche Neculai; Talpeş Marilena; Cristea Victor; Tenciu Magdalena

    2013-01-01

    Using anammox process in biological filtration of technological water within recirculatingaquaculture system is an alternative to nitrification / denitrification process which can reduce productioncosts, especially energy by replacing aerobic biological processes that require high oxygen consumption,provided through the addition for optimal function of biofilter (conversion of N-NH4 and N-NO2). Aim ofthis paper is to highlight the first steps in the control of nitrogen compounds in recirculat...

  3. 2-Methylisoborneol and geosmin uptake by organic sludge derived from a recirculating aquaculture system.

    Guttman, Lior; van Rijn, Jaap

    2009-02-01

    In a previous study on a recirculating fish culture system, levels of geosmin and 2-methylisoborneol were found to decrease when culture water was recirculated through the anaerobic sludge digestion treatment stage of the system. This finding led us to the present study in which the geosmin and 2-methylisoborneol removal capacity of the sludge derived from this treatment stage was examined in vitro. It was found that reduction of off-flavor compounds by the sludge was mediated by both chemical/physical sorption and biological degradation. At geosmin and 2-methylisoborneol concentrations within the range of those experienced in fish culture systems, chemical/physical sorption by the sludge was found to account for a 93% reduction in geosmin and a 79% reduction in 2-methylisoborneol from the overlying water within 48h of incubation. Combined with the biological degradation taking place in the sludge, a complete removal of these compounds from the water phase occurred within 9 days of incubation. By means of repeated washing of the geosmin and 2-methylisoborneol contaminate sludge with clean water, relatively small amounts of these compounds were released from the sludge, a possible indication for the fact that absorption, rather than adsorption, underlies the chemical/physical removal process. PMID:18986667

  4. Fuzzy logic based control system for fresh water aquaculture: A MATLAB based simulation approach

    Rana Dinesh Singh

    2015-01-01

    Full Text Available Fuzzy control is regarded as the most widely used application of fuzzy logic. Fuzzy logic is an innovative technology to design solutions for multiparameter and non-linear control problems. One of the greatest advantages of fuzzy control is that it uses human experience and process information obtained from operator rather than a mathematical model for the definition of a control strategy. As a result, it often delivers solutions faster than conventional control design techniques. The proposed system is an attempt to apply fuzzy logic techniques to predict the stress factor on the fish, based on line data and rule base generated using domain expert. The proposed work includes a use of Data acquisition system, an interfacing device for on line parameter acquisition and analysis, fuzzy logic controller (FLC for inferring the stress factor. The system takes stress parameters on the fish as inputs, fuzzified by using FLC with knowledge base rules and finally provides single output. All the parameters are controlled and calibrated by the fuzzy logic toolbox and MATLAB programming.

  5. 基于PLC循环水养殖温控系统的设计%The Design of Recirculating Aquaculture Temperature Control System Based on PLC

    吴燕翔; 胡咏梅; 刘雨青

    2011-01-01

    针对目前国内水产养殖监控系统较落后的研究现状,设计了采用西门子S7-200PLC的循环水养殖温控系统,采用PID控制算法,对养殖池的水位和温度进行实时监控,实现养殖池温度和水位的恒定控制.详细介绍了系统的整体控制方案,工作原理,并给出了系统的软、硬件结构设计,有效地实现了系统的逻辑控制、安全控制、故障显示及故障处理.%Since study on the domestic aquaculture monitoring system was backward at present, the SIMATIC S7-200 CPU226 is used and adopted PID control algorithm to make real-time monitoring and controlling for the water level and temperature of closed circulating aquaculture system, thus it realized constant water level and temperature control in the culture pond. The system overall control scheme and principle are introduced in detail, and the system software and hardware structure design are given, the system has efficiently implemented system logic control, safetv control, fault display and fault treatment.

  6. 欧洲循环水养殖系统研究进展%Developments of recirculating aquaculture systems in Europe

    丁建乐; 鲍旭腾; 梁澄

    2011-01-01

    The innovation and development of recirculating aquaculture system ( RAS) technology in Europe keeps continuing, with the strengthening of environmental regulations in EU and European countries. This paper introduced and analyzed the development status of European recirculating aquaculture system, such as the application of Life Cycle Assessment (LCA) method for evaluating the environmental impact of RAS, and the increasing species diversity of RAS production, etc. The paper also highlighted the application of denitrifying reaction technology, sludge concentration technology, and ozone treatment technology in RAS. On this basis, the paper pointed out the new challenges with which the recirculating aquaculture system may be faced.%欧洲循环水养殖系统技术随着欧盟和欧洲各国环保法规的加强而不断创新和发展.本文对欧洲正在使用生命周期法对循环水养殖进行环境影响评估的状况,以及欧洲应用独特的循环水养殖系统技术增加了养殖的品种进行了介绍和分析.并着重介绍了欧洲循环水养殖系统新技术的应用及其发展状况,特别阐述了脱硝反应技术、淤泥浓缩技术和臭氧技术等新技术在循环水养殖系统的应用情况.在此基础上,指出了循环水养殖可能面临的新挑战.

  7. Preliminary research on the anammox process and control of nitrogen compounds in a recirculating aquaculture system

    Savin Cristian

    2013-01-01

    Full Text Available Using anammox process in biological filtration of technological water within recirculatingaquaculture system is an alternative to nitrification / denitrification process which can reduce productioncosts, especially energy by replacing aerobic biological processes that require high oxygen consumption,provided through the addition for optimal function of biofilter (conversion of N-NH4 and N-NO2. Aim ofthis paper is to highlight the first steps in the control of nitrogen compounds in recirculating aquaculturesystems using anammox process. Data shown are obtained after 78 researching days (research stillongoing, the results being encouraging, registering an ammonium removal efficiency of 77% in SC1 andof 32% in SC2. Nitrites registered an increase in SC1 and a decrease in SC2 and there are allprerequisites showing that in SC2 anammox activity is more advanced than in SC1. The average ofoxygen consumption rate was 0.73 mgL-1 in SC1 smaller than 0.99 mgL-1 from SC2, which reinforces theidea that there are anammox process in SC2.

  8. Results regarding growth performance of Nile tilapia (Oreochromis niloticus, Linnaeus, 1758) fed with an additive feed, vitamin C, in a recirculating aquaculture system.

    Catalina Ciortan Mirea

    2013-01-01

    The aim of this study was to determine the vitamin C status on growth performance, survival rate and biochimical parameters from tissue of Nile tilapia (Oreochromis niloticus, Linnaeus, 1758) with average weight of 93±1,0 g were fed with different dietary vitamin C rations and reared in a recirculating aquaculture system (RAS). Growth performance , survival rate and biochimical parameters from tissue of Nile tilapia were measured in groups fed diets supplemented with 100, 750, 2000 mg.kg-1L-a...

  9. Cribrihabitans marinus gen. nov., sp. nov., isolated from a biological filter in a marine recirculating aquaculture system.

    Chen, Zhu; Liu, Ying; Liu, Liang-Zi; Zhong, Zhi-Ping; Liu, Zhi-Pei; Liu, Ying

    2014-04-01

    A Gram-negative bacterium, strain CZ-AM5(T), was isolated from an aerated biological filter in a marine recirculating aquaculture system in Tianjin, China. Its taxonomic position was investigated by using a polyphasic approach. Cells of strain CZ-AM5(T) were non-spore-forming rods, 0.5-0.8 µm wide and 1.2-2.0 µm long, and motile by means of one or two polar or lateral flagella. Strain CZ-AM5(T) was strictly aerobic, heterotrophic, oxidase-negative and catalase-positive. Growth occurred at 15-40 °C (optimum, 30-35 °C), at pH 6.5-10.5 (optimum, pH 7.0-7.5) and in the presence of 0-12.0 % (w/v) NaCl (optimum, 4.0 %). The predominant fatty acid was C18 : 1ω7c (80.3 %). Ubiquinone 10 (Q-10) was the sole respiratory quinone. The polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, an unknown aminolipid, an unknown phospholipid and three unknown lipids. The DNA G+C content was 60.4 mol%. Strain CZ-AM5(T) showed the highest 16S rRNA gene sequence similarity (96.5 %) to Phaeobacter caeruleus LMG 24369(T); it exhibited 16S rRNA gene sequence similarity of 95.0-96.5, 95.2-96.3, 96.2, 94.6-95.7 and 94.8-95.8 % to members of the genera Phaeobacter, Ruegeria, Citreimonas, Leisingera and Donghicola, respectively. However, phylogenetic trees based on 16S rRNA gene sequences showed that strain CZ-AM5(T) did not join any of the above genera, but formed a distinct lineage in the trees. On the basis of phenotypic, chemotaxonomic and phylogenetic analyses, strain CZ-AM5(T) is considered to represent a novel genus and species of the family Rhodobacteraceae, for which the name Cribrihabitans marinus gen. nov., sp. nov. is proposed. The type strain of Cribrihabitans marinus is CZ-AM5(T) ( = CGMCC 1.13219(T) = JCM 19401(T)). PMID:24425741

  10. 封闭循环水养殖中曝气系统设计及曝气器的选择%Aeration system design and aeration devices selection in Recirculating Aquaculture System

    宋奔奔; 吴凡; 倪琦; 张宇雷; 庄保陆

    2011-01-01

    随着水产养殖业逐渐向高密度、集约化方向的发展,对养殖水体溶解氧浓度的要求越来越高.目前对循环水高密度养殖水体中溶解氧收支平衡的基础研究较少.如何提高氧气的溶解效率、减少氧气的逸出、合理控制溶解氧水平等技术难关的突破,对循环水养殖技术的提高具有重大的意义.本文旨在通过阐述曝气技术的设计理论基础以及曝气设备的最近发展状况,比较各式曝气器的性能参数,从而为封闭循环水养殖曝气系统的设计和曝气器的选择提供理论支持,推动封闭式循环水养殖向精准化的环保节能方向发展.%Dissolved oxygen in water body plays an important role in recirculating aquaculture while developing to higher density and intensification. At present, it' s studied not so much about dissolved oxygen budget and mass balance in intensive recirculating aquaculture system. These problems such as how to improve oxygen dissolving efficiency, decrease oxygen loss and control dissolved oxygen in appropriate level are growing more prominent in recirculating aquaculture system technology. Aeration theory and designing principles, aeration devices characteristics are illustrated and elaborated in this paper to improve the design of recirculating aquaculture aeration system and the selecting of aeration devices, promoting recirculating aquaculture system technology to more environment-friendly and energy conservation.