Sample records for anti-mullerian hormone

  1. Anti-Mullerian hormone and ovarian dysfunction

    Broekmans, Frank J.; Visser, Jenny A.; Laven, Joop S. E.; Broer, Simone L.; Themmen, Axel P. N.; Fauser, Bart C.


    Anti-Mullerian hormone (AMH) has important roles in postnatal ovarian function. Produced by ovarian granulosa cells, AMH is involved in initial follicle development. In fact, serum AMH level correlates with ovarian follicle number. In patients with polycystic ovary syndrome (PCOS), AMH levels are el

  2. Anti-Mullerian hormone normogram in an Irish subfertile population.

    Naasan, M N


    There has been much interest in the use of anti-Mullerian hormone (AMH) as a biomarker in the assessment of ovarian reserve, and debate on its use as a predictor of assisted reproductive technology (ART) outcomes. Normal levels have not been well defined, and age-based reference ranges may have a role in counselling patients.

  3. Anti-Mullerian hormone in health and disease: a review

    Mangala Sirsikar


    Full Text Available Anti-Mullerian hormone (AMH is a homodimeric glycoprotein, member of the transforming growth factor beta family of growth and differentiation factors. In the ovary, AMH has an inhibitory effect on primordial follicle recruitment as well as on the responsiveness of growing follicles to follicle-stimulating hormone (FSH. The ovary-specific expression pattern in granulosa cells of growing non-selected follicles makes AMH an ideal marker for the size of the ovarian follicle pool. AMH levels accurately reflect the ovarian follicular reserve and could, therefore, be considered as an extremely sensitive marker of ovarian aging and a valuable tool in the diagnosis and the recognition of recurrence of granulosa cell tumors. Furthermore, AMH could be a surrogate diagnostic marker of polycystic ovary syndrome in cases in which ultrasonographic examination is not possible. Additionally AMH evaluation is of clinical importance in predicting the success of in vitro fertilization (IVF. Special reference is made to the possible implications of AMH in the pathogenesis of polycystic ovary syndrome and the relationship between AMH and obesity. AMH also plays important role in evaluation of infants with ambiguous genitalia and other intersex conditions. This article is a review of the clinical usefulness of AMH evaluation in the fields of gynecological endocrinology, menopause, gynecological oncology and assisted reproduction and also in pediatric patients. [Int J Res Med Sci 2016; 4(7.000: 2514-2520

  4. Anti-mullerian hormon level and polycystic ovarian syndrome diagnosis

    Shahrzad Zadehmodarres


    Full Text Available Background: Polycystic ovarian syndrome (PCOS is a common endocrinopathy that accompanied with long term complications. The early diagnosis of this syndrome can prevent it. Objective: The aim was to determine the role of anti-mullerian hormon (AMH in PCOS diagnosis and to find cut off level of it. Materials and Methods: In this cross sectional study, 117 women between 20-40 years old were participated in two groups: 60 PCOS women (based on Rotterdam criteria consensus as the case group and 57 normal ovulatory women as the control group. In day 2-4 of cycle, transvaginal sonography was performed and serum hormonal level of AMH, luteinizing hormone (LH, follicle stimulating hormone (FSH, estradiol (E2, testosterone, fasting blood sugar (FBS, thyroid stimulating hormone (TSH, and prolactin (PRL were measured in all of participants. For all of them score of hirsutism (base on Freeman-Galloway scoring was determined. Results: There were statistically significant in irregular pattern of menstruation, AMH and FSH level, and presence of hirsutism between two groups. But regarding mean of age, body mass index, plasma level of PRL, TSH, LH, Testosterone, FBS, and E2 differences were not significant. Construction by ROC curve present 3.15 ng/ml as AMH cut off with 70.37% sensitivity and 77.36% specificity in order to PCOS diagnosis. Conclusion: AMH with cut off level of 3.15 ng/ml with sensitivity 70.37% and specificity 77.36% could use for early diagnosis of PCOS patients.

  5. Anti-Mullerian hormone : a marker for oocyte quantity, oocyte quality and embryo quality?

    Fong, S. Lie; Baart, E. B.; Martini, E.; Schipper, I.; Visser, J. A.; Themmen, A. P. N.; de Jong, F. H.; Fauser, B. J. C. M.; Laven, J. S. E.


    Serum anti-Mullerian hormone (AMH) concentrations decline with increasing age and constitute a Sensitive marker for ovarian ageing. In addition, basal serum AMH concentrations predict ovarian response during IVF cycles. Concomitantly, oocyte quantity and embryo quality decrease with advancing age. H

  6. Anti-Mullerian hormone (AMH) : what do we still need to know?

    La Marca, A.; Broekmans, F. J.; Volpe, A.; Fauser, B. C.; Macklon, N. S.


    In the ovary, Anti-Mullerian hormone (AMH) is produced by the granulosa cells of early developing follicles and inhibits the transition from the primordial to the primary follicular stage. AMH levels can be measured in serum and have been shown to be proportional to the number of small antral follic

  7. Anti-Mullerian hormone and response to ovulation induction with clomiphene citrate in women with polycystic ovary syndrome

    Mohamed S. Sweed


    Conclusions: Anti-Mullerian hormone is a very useful predictor of poor responders to clomiphene citrate among women with polycystic ovary disease. [Int J Reprod Contracept Obstet Gynecol 2016; 5(3.000: 603-608

  8. Anti-Mullerian-hormone levels during pregnancy and postpartum

    Köninger, Angela; Kauth, Alexis; Schmidt, Boerge; Schmidt, Markus; Yerlikaya, Guelen; Kasimir-Bauer, Sabine; Kimmig, Rainer; Birdir, Cahit


    Background The number of unintentionally childless couples is increasing as more couples seek to conceive for the first time in the third or fourth decade of the woman’s life. Determination of ovarian reserve is an essential component of infertility assessment. The Anti-Müllerian-Hormone (AMH) seems to be the most reliable predictor of ovarian reserve. In this study we analyzed AMH in a cohort of pregnant women without fertility impairment to determine age-dependent decline and possible AMH f...

  9. Expression of anti-Mullerian hormone receptor on the appendix testis in connection with urological disorders

    Kornél Kistamás; Olga Ruzsnavszky; Andrea Telek; Lívia Kosztka; Ilona Kovács; Beatrix Dienes; László Csernoch


    The female internal sex organs develop from the paramesonephric (Mullerian) duct.In male embryos,the regression of the Mullerian duct is caused by the anti-Mullerian hormone (AMH),which plays an important role in the process of testicular descent.The physiological remnant of the Mullerian duct in males is the appendix testis (AT).In our previous study,we presented evidence for the decreased incidence of AT in cryptorchidism with intraoperative surgery.In this report,the expression of the anti-Mullerian hormone receptor type 2 (AMHR2),the specific receptor of AMH,on the AT was investigated in connection with different urological disorders,such as hernia inguinalis,torsion of AT,cysta epididymis,varicocele,hydrocele testis and various forms of undescended testis.The correlation between the age of the patients and the expression of the AMHR2 was also examined.Reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry were used to detect the receptor's mRNA and protein levels,respectively.We demonstrate that AMHR2 is expressed in the ATs.Additionally,the presence of this receptor was proven at the mRNA and protein levels.The expression pattern of the receptor correlated with neither the examined urological disorders nor the age of the patients;therefore,the function of the AT remains obscure.

  10. Changes in anti-Mullerian hormone serum concentrations over time suggest delayed ovarian ageing in normogonadotrophic anovulatory infertility

    Mulders, AGMGJ; Laven, JSE; Eijkemans, MJC; de Jong, FH; Themmen, APN; Fauser, BCJM


    BACKGROUND: Anti-Mullerian hormone (AMH), produced by growing pre-antral and early antral ovarian follicles, has been shown to be a useful marker for ovarian ageing. Serum AMH concentrations are elevated during reproductive life in anovulatory women, especially in those patients exhibiting polycysti

  11. Genetic variants in anti-Mullerian hormone and anti-Mullerian hormone receptor genes and breast cancer risk in Caucasians and African Americans.

    Nan, Hongmei; Dorgan, Joanne F; Rebbeck, Timothy R


    Anti-Mullerian hormone (AMH) regulates ovarian folliculogenesis by signaling via its receptors, and elevated serum AMH levels are associated with an increased risk of breast cancer. No previous studies have examined the effects of genetic variants in AMH-related genes on breast cancer risk. We evaluated the associations of 62 single nucleotide polymorphisms (SNPs) in AMH and its receptor genes, including AMH type 1 receptor (ACVR1) and AMH type 2 receptor (AMHR2), with the risk of breast cancer in the Women's Insights and Shared Experiences (WISE) Study of Caucasians (346 cases and 442 controls), as well as African Americans (149 cases and 246 controls). Of the 62 SNPs evaluated, two showed a nominal significant association (P for trend < 0.05) with breast cancer risk among Caucasians, and another two among African Americans. The age-adjusted additive odds ratios (ORs) (95% confidence interval (95% CI)) of those two SNPs (ACVR1 rs12694937[C] and ACVR1 rs2883605[T]) for the risk of breast cancer among Caucasian women were 2.33 (1.20-4.52) and 0.68 (0.47-0.98), respectively. The age-adjusted additive ORs (95% CI) of those two SNPs (ACVR1 rs1146031[G] and AMHR2 functional SNP rs2002555[G]) for the risk of breast cancer among African American women were 0.63 (0.44-0.92) and 1.67 (1.10-2.53), respectively. However, these SNPs did not show significant associations after correction for multiple testing. Our findings do not provide strong supportive evidence for the contribution of genetic variants in AMH-related genes to the risk of developing breast cancer in either Caucasians or African Americans. PMID:25379134

  12. Comparison of anti-mullerian hormone level in non-endometriotic benign ovarian cyst before and after laparoscopic cystectomy

    Sedigheh Amooee; Mahboubeh Gharib; Parsa Ravanfar


    Background: Benign ovarian cysts are common among both pre- and postmenstrual women. Surgical intervention for excision of an ovarian cyst is mandated when symptomatic, or chance for malignancy is high. The damaging effect of surgical ovarian cystectomy on ovarian reserve is debated in recent studies. Objective: In the present study we investigated serum level of anti-mullerian hormone (AMH) as an indicator of ovarian reserve before and after surgical cystectomy. Materials and Methods: ...

  13. Anti-Mullerian Hormone: Above and Beyond Conventional Ovarian Reserve Markers

    Jamil, Zehra; Fatima, Syeda Sadia; Ahmed, Khalid; Malik, Rabia


    Management of ovarian dysfunctions requires accurate estimation of ovarian reserve (OR). Therefore, reproductive hormones and antral follicle count (AFC) are assessed to indicate OR. Serum anti-Mullerian hormone (AMH) is a unique biomarker that has a critical role in folliculogenesis as well as steroidogenesis within ovaries. Secretion from preantral and early antral follicles renders AMH as the earliest marker to show OR decline. In this review we discuss the dynamics of circulating AMH that remarkably vary with sex and age. As it emerges as a marker of gonadal development and reproductive disorders, here we summarize the role of AMH in female reproductive physiology and provide evidence of higher accuracy in predicting ovarian response to stimulation. Further, we attempt to compile potential clinical applications in children and adults. We propose that AMH evaluation has a potential role in effectively monitoring chemotherapy and pelvic radiation induced ovarian toxicity. Furthermore, AMH guided ovarian stimulation can lead to individualization of therapeutic strategies for infertility treatment. However future research on AMH levels within follicular fluid may pave the way to establish it as a marker of “quality” besides “quantity” of the growing follicles. PMID:26977116

  14. Anti-Mullerian Hormone: Above and Beyond Conventional Ovarian Reserve Markers

    Zehra Jamil


    Full Text Available Management of ovarian dysfunctions requires accurate estimation of ovarian reserve (OR. Therefore, reproductive hormones and antral follicle count (AFC are assessed to indicate OR. Serum anti-Mullerian hormone (AMH is a unique biomarker that has a critical role in folliculogenesis as well as steroidogenesis within ovaries. Secretion from preantral and early antral follicles renders AMH as the earliest marker to show OR decline. In this review we discuss the dynamics of circulating AMH that remarkably vary with sex and age. As it emerges as a marker of gonadal development and reproductive disorders, here we summarize the role of AMH in female reproductive physiology and provide evidence of higher accuracy in predicting ovarian response to stimulation. Further, we attempt to compile potential clinical applications in children and adults. We propose that AMH evaluation has a potential role in effectively monitoring chemotherapy and pelvic radiation induced ovarian toxicity. Furthermore, AMH guided ovarian stimulation can lead to individualization of therapeutic strategies for infertility treatment. However future research on AMH levels within follicular fluid may pave the way to establish it as a marker of “quality” besides “quantity” of the growing follicles.

  15. Anti-Mullerian Hormone: Above and Beyond Conventional Ovarian Reserve Markers.

    Jamil, Zehra; Fatima, Syeda Sadia; Ahmed, Khalid; Malik, Rabia


    Management of ovarian dysfunctions requires accurate estimation of ovarian reserve (OR). Therefore, reproductive hormones and antral follicle count (AFC) are assessed to indicate OR. Serum anti-Mullerian hormone (AMH) is a unique biomarker that has a critical role in folliculogenesis as well as steroidogenesis within ovaries. Secretion from preantral and early antral follicles renders AMH as the earliest marker to show OR decline. In this review we discuss the dynamics of circulating AMH that remarkably vary with sex and age. As it emerges as a marker of gonadal development and reproductive disorders, here we summarize the role of AMH in female reproductive physiology and provide evidence of higher accuracy in predicting ovarian response to stimulation. Further, we attempt to compile potential clinical applications in children and adults. We propose that AMH evaluation has a potential role in effectively monitoring chemotherapy and pelvic radiation induced ovarian toxicity. Furthermore, AMH guided ovarian stimulation can lead to individualization of therapeutic strategies for infertility treatment. However future research on AMH levels within follicular fluid may pave the way to establish it as a marker of "quality" besides "quantity" of the growing follicles. PMID:26977116

  16. Anti-Mullerian hormone and antral follicle count as predictors of ovarian reserve and successful IVF

    Ibrahim A Abdelazim; Maha M Belal; Hanan H Makhlouf


    Objective:To investigate the role ofAnti-Mullerian hormone and antral follicle count in predicting the ovarian reserve, and success ofIVF.Methods:Ninety two infertile couples complaining of infertility due to male or tubal or unexplained factors were included in this comparative prospective study forIVF/ICSI.Day-3 basal hormonal level ofFSH,LH,E2, and AMH were measured, followed byTransvaginal ultrasound(TVS) to evaluate theAFC.Controlled ovarian hyperstimulation was done using the long protocol for ovarian hyperstimulation.Results:According to the number of retrieved oocytes women included in this study were classified into two groups; good responders(≥4 retrieved oocytes) and poor responders(<4 retrieved oocytes). Ovarian reserve in this study was assessed by day-3 basal hormonal levels andAFC.The mean Day-3AMH and meanAFC were significantly high(4.93±1.22) ng/mL, and(12.72±5.70) ng/mL; respectively) in good responders compared with poor responders, also, the number of retrieved oocytes were significantly high in the good responders group compared with poor responders (13.52±9.70) versus(3.91±1.20)(P<0.05).The numbers of chemical and clinical pregnancies were significantly high(6 cases(75%) and13 cases(72.2%); respectively) in the good responders compared with poor responders(2 cases(25%) and5 cases(27.8%); respectively).Conclusions:Day-3AMH andAFC are good predictors for ovarian reserve, there were positively correlated with the number of retrieved oocytes and numbers of chemical and clinical pregnancies.

  17. Expression and regulation of anti-mullerian hormone in an oviparous species, the hen.

    Johnson, P A; Kent, T R; Urick, M E; Giles, J R


    Anti-mullerian hormone (AMH) has a critical role in regression of the mullerian duct system during development in male mammalian and avian species and in regression of the right oviduct in female avian species. AMH in adult female birds has not been investigated. Chicken-specific cDNA primers were used to isolate Amh by RT-PCR. This probe was used in Northern blot analysis to identify a 2.8-kb band with expression in total ovarian RNA and in granulosa cell RNA. Quantitative real-time PCR was used to assess Amh expression in follicles of different maturity (1, 3, 5, and 6-12 mm and the largest F1 follicle; n = 4-6 of each size). There was an increased amount of Amh mRNA in the granulosa layer of the smaller follicles and a lower amount in the granulosa layer of the larger follicles (P expression between the germinal disc and non-germinal disc region of 6- to 12-mm follicles, although expression differed with follicle size (P estradiol (E(2)) and progesterone (P(4)), and Amh mRNA was assessed. Neither E(2) nor P(4) influenced Amh mRNA accumulation. Granulosa cells were also cultured in the presence of oocyte-conditioned medium (OCM), which decreased Amh mRNA expression in a dose-related manner (P receptor expression was not affected. Heat treatment of OCM abolished the effect, but growth differentiation factor 9 antiserum did not block the suppression. Immunohistochemistry confirmed that the granulosa layer was the predominant source of AMH in the small follicles of the hen and indicated that AMH was present early in follicle development, with expression in very small follicles (approximately 150 mum). PMID:17881771

  18. Anti-mullerian hormone and antral follicle count as predictors of ovarian response in assisted reproduction

    Y Himabindu


    Full Text Available Objective: The objective of this study was to test the hypothesis that AMH and antral follicle count (AFC are good predictors of ovarian response to controlled ovarian stimulation and to compare them. Materials and Methods: This observational cross-sectional study included 56 subjects aged between 25 and 42 years who were enrolled between 1 st January and 31 st December 2010 for their first intracytoplasmic sperm injection (ICSI program. Baseline hormone profiles including serum levels of Estradiol (E2, Follicle-stimulating hormone (FSH, Luteinizing hormone (LH, and Anti-mullerian Hormone (AMH were determined on day 3 of the previous cycle. The antral follicle count measurements were performed on days 3-5 of the same menstrual cycle. Antral follicles within the bilateral ovaries between 2-6 mm were recorded. The subjects were treated with long protocol for ovarian stimulation. Ovulation was induced with 10,000 IU of human chorionic gonadotropin (hCG when at least 3 follicles attained the size of more than 17 mm. Transvaginal oocyte retrieval was performed under ultrasound guidance 36 hours after hCG administration. An oocyte count less than 4 and absence of follicular growth with controlled ovarian hyper stimulation was considered as poor ovarian response. Oocyte count of 4 or more was considered as normal ovarian response. Results: Statistical analysis was performed using SPSS software trail version 16.0. Subjects were divided into 2 groups, depending on the ovarian response. The mean oocyte counts were 12.27 ± 6.06 and 2.22 ± 1.24 in normal and poor responders, respectively, ( P = 001. Multiple regression analysis revealed AMH and antral follicle count as predictors of ovarian response (β coefficient ± SE for AMH was 1.618 ± 0.602 ( P = 0.01 and for AFC, it was, 0.528 ± 0.175 ( P = 0.004. AFC was found to be a better predictor of ovarian response compared to AMH in controlled ovarian hyper stimulation. Conclusion: The observations made

  19. Concentration of anti-Mullerian hormone and inhibin-B in relation to steroids and age in follicular fluid from small antral human follicles

    Andersen, Claus Yding; Rosendahl, M.; Byskov, A.G.


    CONTEXT: Ovaries surgically removed for fertility preservation served as a source of follicle fluid from human small antral follicles. OBJECTIVE: The objective of the study was to measure intrafollicular concentrations of anti-Mullerian Hormone (AMH), inhibin-B, progesterone, androstenedione, tes...

  20. Serum anti mullerian hormone levels: A better hormonal marker of ovarian reserve

    RadhaVembu, Sanjeeva Reddy Nellapalli, Anjalakshi Chandrashekar, Nalini Ganesan R


    Full Text Available Aim: To determine whether Serum AMH is a better hormonal marker of Ovarian Reserve. Objectives: 1. To correlate AMH with FSH and maternal Age and whether AMH is a better predictor of ovarian response than FSH. Materials & Methods: A total of 246 women enrolled for IVF-ICSI fulfilling the selection criteria were recruited for the study at a tertiary ART centre. On day 3 of the cycle serum AMH, FSH were assayed along with LH, E2, TSH and Prolactin. Within 3 months they were subjected to IVF-ICSI. Serum AMH and FSH levels were compared with Age and Oocytes retrieved. Results: All the 246 women enrolled were analysed. The mean age of the women was 30.7 ± 4.5, average number of oocytes retrieved was 11.8 ±7.1. There was a negative correlation of AMH with age (r= -0.28 which is statistically significant where as FSH showed a positive correlation (r= -0.27. With regard to retrieval of mature oocytes, AMH showed a high positive correlation (r= 0.60 which is statistically significant (p <0.000 when compared to serum FSH (r = -0.26. Conclusion: AMH is a better hormonal marker of Ovarian Reserve and a better predictor of Oocytes retrieved than serum FSH levels.

  1. Can anti-Mullerian hormone replace ultrasonographic evaluation in polycystic ovary syndrome? A review of current progress

    Awadhesh Kumar Singh


    Full Text Available Several studies over the past decade have now consistently indicated that the serum anti-Mullerian hormone (AMH levels are at least 2–3-fold higher in the patients with polycystic ovary syndrome (PCOS, which also corresponds to the increased number of AMH producing preantral and small antral follicles. Moreover, AMH levels have been found to be associated in direct proportion to the follicle numbers per ovary or antral follicular count, assessed by the transvaginal ultrasound (TVS. Furthermore, AMH correlates directly with the rising serum testosterone and luteinizing hormone levels in PCOS. Hence, serum AMH in women with oligo-anovulation and/or hyperandrogenemia could indicate the presence of underlying PCOS, when reliable TVS is not feasible, or not acceptable, either due to the virginal status or psycho-social issue. In addition, the imaging quality of abdominal ultrasound is often impaired by obesity, which typically occurs in PCOS women. Indeed, PCOS occurs most commonly in young females who cannot be subjected to invasive TVS for various reasons; therefore, a desirable alternative to TVS is urgently required to diagnose the most prevalent endocrine abnormality of young women. This review will analyze the currently available evidence regarding the role of AMH in the diagnosis of PCOS.

  2. Age-Related Distribution of Basal Anti-Mullerian Hormone Levels in a Population of Infertile Women

    Pinar Ozcan


    Full Text Available Aim: We aimed to constitute age-specific reference serum values for anti-Mullerian Hormone (AMH in women, and to analyze the distribution of basal serum AMH levels in Turkish women of reproductive age attending an infertility clinic to provide a framework for expected values according to age. Material and Method: Retrospective analysis of prospectively collected data on cycle day 2-3 serum AMH measurements of 409 women attending a single infertility unit in Turkey through a 12-month-period was performed. Results: Concentrations of serum AMH were shown to decrease with advancing age of the female partner. The mean age of the women was 34.04±5.39 years and the mean AMH level of the women was 1.77±1.82. The AMH levels were grouped according to age as follows: 20-24, 25-29, 30-34,35-39, and >40 years. The median AMH values were 2.16 ng/ml, 2.15 ng/ml, 1.71 ng/ml, 0.80 ng/ml, and 0.47 ng/ml, respectively according to the age groups. Discussion: The present data provide a framework for age-specific serum AMH levels in a Turkish population of infertile women.

  3. 抗苗勒管激素及其Ⅱ型受体与卵母细胞成熟的关系%Relationship between anti-mullerian hormone, anti-mullerian hormone receptor type Ⅱand oocyte maturation

    姜宙; 王晓燕; 孙贤华; 邹淑花


    Objective:To investigate the relationship among anti-mullerian hormone (AMH),anti-mullerian hormone receptor type Ⅱ expression and oocyte maturation.Methods:Cumulus granular cells,follicular fluids and blood samples were obtained from 32 ovulatory women undergoing intracytoplasmic sperm injection treatment on ovum pick-up day.All the samples were recorded oocyte maturation and the number of retrieved oocytes.The influence of serum and follicular fluids AMH basal levels were evaluated by ELISA.Cumulus GC were assayed by Realtime PCR for AMHR Ⅱ mRNA expression.Results:AMH concentrations in the FF were higher than that of serum and showed significant differences [(3083.78 ±682.19)pg/ml vs (1012.29±423.21) pg/ml,P<0.001].AMH concentrations in the FF and serum level of each retrieved oocytes were found positive correlation(P=0.045).AMH concentrations in the FF of M Ⅱ oocytes were significantly lower than in the FF of M Ⅰ oocytes (P =0.000).AMHR Ⅱ mRNA expression in cumulus GC in M Ⅰ oocytes were 4 times more than M Ⅱ oocytes (P =0.000).Conclusion:AMH may inhibit the maturation of oocyte via type Ⅱ receptor.Serum AMH level of each retrieved oocytes could be used as a diagnostic criterion of oocyte maturation.%目的:探讨抗苗勒管激素(AMH)及其Ⅱ型受体(AMHⅡ)与卵母细胞成熟的关系.方法:选取因男性因素不孕进行卵胞浆内单精子注射(ICSI)治疗的32例患者,于取卵日采集3ml静脉血,收集卵泡液及卵丘颗粒细胞,记录获卵数、卵子成熟度.采用ELISA法测定血清及卵泡液中的AMH水平;Real-time PCR测定卵丘颗粒细胞中AMHⅡ型受体(AMHRⅡ)mRNA的相对表达.结果:卵泡液中的AMH水平[(3083.78±682.19)pg/ml]显著高于血清[(1012.29±423.21) pg/ml] (P<0.001);卵泡液中AMH水平与血清AMH水平/获卵数呈正相关(P=0.045).MⅠ期卵母细胞卵泡液中的AMH水平显著高于MⅡ期(P=0.000);MⅠ期卵丘颗粒细胞中的AMHRⅡmRNA的

  4. Effect of anti-Mullerian hormone in culture medium on quality of mouse oocytes matured in vitro.

    Yihui Zhang

    Full Text Available Anti-mullerian hormone (AMH is thought to reflect the growth of follicles and the ovarian function. However, the role of AMH in culture medium during in vitro maturation (IVM on oocyte quality and subsequent development potential is unclear. The objective of this study is to investigate the effect of recombinant human AMH (rh-AMH supplemented into IVM medium on oocyte quality. Cumulus-oocyte complexes (COCs were obtained from ICR mice and cultured in vitro with the different concentrations (0-1,000 ng/ml of rh-AMH. Following 16-18 h of culture, quantitative PCR and ELISA were performed to analyze GDF9 and BMP15 mRNA expression and protein production from the oocytes. Subsequently, in vitro fertilization (IVF and early embryonic development were employed to further evaluate the quality of in vitro matured oocytes. The results showed that AMH was only expressed in cumulus cells but not in the oocytes. However, AMH most specific receptor, AMHR-II, was expressed in both oocytes and cumulus cells. The levels of GDF9 and BMP15 expression and blastocyst formation rate were significantly increased (p<0.05 when the IVM medium was supplemented with 100 ng/ml of rh-AMH. With AdH1-SiRNA/AMH for knocking down of AMH expression during IVM significantly reduced (p<0.05 the levels of GDF9 and BMP15 expression and blastocysts formation rate. These results suggest that AHM improves oocytes quality by up-regulating GDF9 and BMP15 expressions during IVM.

  5. Systemic and local anti-Mullerian hormone reflects differences in the reproduction potential of Zebu and European type cattle.

    Carter, Anja Stojsin-; Mahboubi, Kiana; Costa, Nathalia N; Gillis, Daniel J; Carter, Timothy F; Neal, Michael S; Miranda, Moyses S; Ohashi, Otavio M; Favetta, Laura A; King, W Allan


    This study was conducted to evaluate plasma anti-Mullerian hormone (Pl AMH), follicular fluid AMH (FF AMH) and granulosa cell AMH transcript (GC AMH) levels and their relationships with reproductive parameters in two cattle subspecies, Bos taurus indicus (Zebu), and Bos taurus taurus (European type cattle). Two-dimensional ultrasound examination and serum collection were performed on Zebu, European type and crossbreed cows to determine antral follicle count (AFC), ovary diameter (OD) and Pl AMH concentration. Slaughterhouse ovaries for Zebu and European type cattle were collected to determine FF AMH concentrations, GC AMH RNA levels, AFC, oocyte number, cleavage and blastocyst rate. Additionally GC AMH receptor 2 (AMHR2) RNA level was measured for European type cattle. Relationship between AMH and reproductive parameters was found to be significantly greater in Zebu compared to European cattle. Average Pl AMH mean±SE for Zebu and European cattle was 0.77±0.09 and 0.33±0.24ng/ml respectively (p=0.01), whereas average antral FF AMH mean±SE for Zebu and European cattle was 4934.3±568.5 and 2977.9±214.1ng/ml respectively (pcattle. Levels of GC AMHR2 RNA in European cattle were correlated to oocyte number (p=0.01). Crossbred animals were found more similar to their maternal Zebu counterparts with respect to their Pl AMH to AFC and OD relationships. These results demonstrate that AMH reflects differences between reproduction potential of the two cattle subspecies therefore can potentially be used as a reproductive marker. Furthermore these results reinforce the importance of separately considering the genetic backgrounds of animals when collecting or interpreting bovine AMH data for reproductive performance. PMID:26898391

  6. Anti-Mullerian Hormone as A Predictive Factor in Assisted Reproductive Technique of Polycystic Ovary Syndrome Patients

    Parvin Fallahi


    Full Text Available This study aimed to assess the relationship between the serum levels of anti-mullerian hormone (AMH and other hormonal markers and results of assisted reproductive techniques (ART in polycystic ovary syndrome (PCOS patients. This cohort study was conducted on 60 PCOS patients who were candidates for assisted reproductive techniques. In all patients the serum levels of AMH, follicle stimulating hormone (FSH and luteinizing hormone (LH, estradiol (E2, free testosterone (fT, testosterone (T and inhibin B were measured in the 3rd day of menstrual cycle. The relationship between serum level of measured hormonal markers with retrieved oocytes, mature oocytes, the number of transferred fetus and pregnancy rate were assessed. The cut-off value for the serum level of AMH and retrieved oocytes were determined. There was a significant direct correlation between the serum mullerian inhibiting substance (MIS level with number of total picked up oocytes (r=0.412, mature oocytes (r=0.472 and embryo transfer (r=0.291. There was a linear and significant correlation between inhibin B and fertilization (r=0.283 Cut-off point for AMH level according to presence or absence of pregnancy was 4.8 ng/ml and it was not statistically significant (P=0.655. Area under curve (AUC was 0.543. Cut-off point for MIS according to picked up oocytes was 2.7 ng/ml with area under the curve (ROC curve of 0.724 (CI= 0.591-0.831 (P=0.002. Patients with PCOS who had AMH more than 2.7 ng/ml, the number of retrieved oocytes (6 or more was higher than MIS/AMH <2.7 ng/ml (P=0.002. As a marker of ovarian responsiveness to controlled ovarian hyperstimulation (COH and despite a small sample size of our study, it is revealed that pretreatment MIS/AMH is highly associated with the number of mature oocytes retrieved during COH in PCOS women.

  7. Testicular anti-mullerian hormone secretion is stimulated by recombinant human FSH in patients with congenital hypogonadotropic hypogonadism.

    Young, Jacques; Chanson, Philippe; Salenave, Sylvie; Noël, Michèle; Brailly, Sylvie; O'Flaherty, Martín; Schaison, Gilbert; Rey, Rodolfo


    Serum anti-Mullerian hormone (AMH), a prepubertal Sertoli cell marker, declines during puberty as an early sign of testicular testosterone (T) production. When T synthesis or action is impaired, serum AMH is abnormally high in the first months after birth and at puberty but normal between these two periods. We postulated that FSH might be responsible for AMH up-regulation in the absence of androgen inhibition. To test this hypothesis, we administered recombinant human (rh) FSH to eight patients aged from 18-31 yr with untreated congenital hypogonadotropic hypogonadism. This situation is ideal to study the effect of FSH on AMH production because it avoids interference by endogenous gonadotropins and T. The patients received daily sc injections of 150 IU rhFSH for 1 month, followed in seven of them by a combined treatment of rhFSH plus human chorionic gonadotropin (hCG; 1500 UI im, twice a week) for 2 months. Gonadotropins, T, AMH, and inhibin B were measured in plasma before treatment every 10 d during rhFSH treatment and every month during combined rhFSH and hCG treatments. All hormones were at prepubertal levels before treatment. Although LH and T did not vary, AMH and inhibin B levels gradually increased after 20 d of FSH administration. However, in contrast to rhFSH alone, the combined rhFSH plus hCG stimulation of the testis dramatically suppresses the secretion of AMH and induced a modest but significant reduction of circulating inhibin B levels. We conclude that FSH stimulates AMH production in the testis when it is at a prepubertal stage. In addition, the decrease of serum AMH during combined rhFSH and hCG testicular stimulation is in agreement with the concept that during pubertal development and in adult life, the suppressive effect of LH-driven testicular androgens outweighs the stimulating effect of FSH on AMH production by Sertoli cells. Finally, the hCG-induced decrease in inhibin B suggests that in humans, as previously demonstrated in monkeys

  8. Anti-mullerian hormone is not associated with cardiometabolic risk factors in adolescent females.

    Emma L Anderson

    Full Text Available Epidemiological evidence for associations of Anti-Müllerian hormone (AMH with cardiometabolic risk factors is lacking. Existing evidence comes from small studies in select adult populations, and findings are conflicting. We aimed to assess whether AMH is associated with cardiometabolic risk factors in a general population of adolescent females.AMH, fasting insulin, glucose, HDLc, LDLc, triglycerides and C-reactive protein (CRP were measured at a mean age 15.5 years in 1,308 female participants in the Avon Longitudinal Study of Parents and Children (ALSPAC. Multivariable linear regression was used to examine associations of AMH with these cardiometabolic outcomes.AMH values ranged from 0.16-35.84 ng/ml and median AMH was 3.57 ng/ml (IQR: 2.41, 5.49. For females classified as post-pubertal (n = 848 at the time of assessment median (IQR AMH was 3.81 ng/ml (2.55, 5.82 compared with 3.25 ng/ml (2.23, 5.05 in those classed as early pubertal (n = 460, P≤0.001. After adjusting for birth weight, gestational age, pubertal stage, age, ethnicity, socioeconomic position, adiposity and use of hormonal contraceptives, there were no associations with any of the cardiometabolic outcomes. For example fasting insulin changed by 0% per doubling of AMH (95%CI: -3%,+2% p  = 0.70, with identical results if HOMA-IR was used. Results were similar after additional adjustment for smoking, physical activity and age at menarche, after exclusion of 3% of females with the highest AMH values, after excluding those that had not started menarche and after excluding those using hormonal contraceptives.Our results suggest that in healthy adolescent females, AMH is not associated with cardiometabolic risk factors.

  9. Inhibitory actions of Anti-Mullerian Hormone (AMH on ovarian primordial follicle assembly.

    Eric E Nilsson

    Full Text Available The current study was designed to investigate the actions of Anti-Müllerian Hormone (AMH on primordial follicle assembly. Ovarian primordial follicles develop from the breakdown of oocyte nests during fetal development for the human and immediately after birth in rodents. AMH was found to inhibit primordial follicle assembly and decrease the initial primordial follicle pool size in a rat ovarian organ culture. The AMH expression was found to be primarily in the stromal tissue of the ovaries at this period of development, suggesting a stromal-epithelial cell interaction for primordial follicle assembly. AMH was found to promote alterations in the ovarian transcriptome during primordial follicle assembly with over 200 genes with altered expression. A gene network was identified suggesting a potential central role for the Fgf2/Nudt6 antisense transcript in the follicle assembly process. A number of signal transduction pathways are regulated by AMH actions on the ovarian transcriptome, in particular the transforming growth factor-beta (TGFß signaling process. AMH is the first hormone/protein shown to have an inhibitory action on primordial follicle assembly. Due to the critical role of the primordial follicle pool size for female reproduction, elucidation of factors, such as AMH, that regulate the assembly process will provide insights into potential therapeutics to manipulate the pool size and female reproduction.

  10. Anti-Mullerian hormone recruits BMPR-IA in immature granulosa cells.

    Lauriane Sèdes

    Full Text Available Anti-Müllerian hormone (AMH is a member of the TGF-β superfamily secreted by the gonads of both sexes. This hormone is primarily known for its role in the regression of the Müllerian ducts in male fetuses. In females, AMH is expressed in granulosa cells of developing follicles. Like other members of the TGF-β superfamily, AMH transduces its signal through two transmembrane serine/threonine kinase receptors including a well characterized type II receptor, AMHR-II. The complete signalling pathway of AMH involving Smads proteins and the type I receptor is well known in the Müllerian duct and in Sertoli and Leydig cells but not in granulosa cells. In addition, few AMH target genes have been identified in these cells. Finally, while several co-receptors have been reported for members of the TGF-β superfamily, none have been described for AMH. Here, we have shown that none of the Bone Morphogenetic Proteins (BMPs co-receptors, Repulsive guidance molecules (RGMs, were essential for AMH signalling. We also demonstrated that the main Smad proteins used by AMH in granulosa cells were Smad 1 and Smad 5. Like for the other AMH target cells, the most important type I receptor for AMH in these cells was BMPR-IA. Finally, we have identified a new AMH target gene, Id3, which could be involved in the effects of AMH on the differentiation of granulosa cells and its other target cells.

  11. Ovarian antral follicle subclasses and anti-mullerian hormone during normal reproductive aging

    Bentzen, J G; Forman, J L; Johannsen, T H;


    CONTEXT: The interindividual variation in the age-related decline of ovarian follicles is wide. Hence, it is important to identify reliable, sensitive, and specific markers to assess the ovarian reserve of the individual woman. OBJECTIVE: The aim of this study was to characterize the relation...... between age and ovarian reserve parameters in a population of healthy women with regular menstrual cycle. DESIGN AND SETTING: We conducted a prospective, population-based, cross-sectional study. PARTICIPANTS: A total of 366 health care workers aged 21-41 years employed at a University Hospital were...... included. Interventions: There were no interventions. MAIN OUTCOME MEASURES: Serum anti-Müllerian hormone (AMH) concentration, antral follicle count (AFC), antral follicle size categories (small: 2-4 mm; intermediate: 5-7 mm; and large: 8-10 mm), and ovarian volume were measured. RESULTS: Serum AMH level...

  12. Which follicles make the most anti-Mullerian hormone in humans?

    Jeppesen, J V; Anderson, R A; Kelsey, T W;


    Anti-Müllerian hormone (AMH) is exclusively produced by granulosa cells (GC) of the developing pre-antral and antral follicles, and AMH is increasingly used to assess ovarian function. It is unclear which size follicles make the most AMH (total content) and are the main contributors to circulating...... AMH concentrations. To determine AMH gene expression in GC (q-RT-PCR) and follicular AMH production (Elisa and RIA) in relation to follicular development, 87 follicles (3-13 mm diameter) including both GC and the corresponding follicular fluid (FF) were collected in connection with fertility...... preservation of human ovaries. Further, follicle number and diameter, graded in 1 mm increments, were determined by 3D ultrasound in 113 women in their natural menstrual cycle to determine follicle number and diameter in relation to circulating AMH levels. This study demonstrates for the first time a positive...

  13. A validated model of serum anti-mullerian hormone from conception to menopause.

    Thomas W Kelsey

    Full Text Available BACKGROUND: Anti-Müllerian hormone (AMH is a product of growing ovarian follicles. The concentration of AMH in blood may also reflect the non-growing follicle (NGF population, i.e. the ovarian reserve, and be of value in predicting reproductive lifespan. A full description of AMH production up to the menopause has not been previously reported. METHODOLOGY/PRINCIPAL FINDINGS: By searching the published literature for AMH concentrations in healthy pre-menopausal females, and using our own data (combined n = 3,260 we have generated and robustly validated the first model of AMH concentration from conception to menopause. This model shows that 34% of the variation in AMH is due to age alone. We have shown that AMH peaks at age 24.5 years, followed by a decline to the menopause. We have also shown that there is a neonatal peak and a potential pre-pubertal peak. Our model allows us to generate normative data at all ages. CONCLUSIONS/SIGNIFICANCE: These data highlight key inflection points in ovarian follicle dynamics. This first validated model of circulating AMH in healthy females describes a transition period in early adulthood, after which AMH reflects the progressive loss of the NGF pool. The existence of a neonatal increase in gonadal activity is confirmed for females. An improved understanding of the relationship between circulating AMH and age will lead to more accurate assessment of ovarian reserve for the individual woman.

  14. Bivariate analysis of basal serum anti-Mullerian hormone measurements and human blastocyst development after IVF

    Sills, E Scott


    Abstract Background To report on relationships among baseline serum anti-Müllerian hormone (AMH) measurements, blastocyst development and other selected embryology parameters observed in non-donor oocyte IVF cycles. Methods Pre-treatment AMH was measured in patients undergoing IVF (n = 79) and retrospectively correlated to in vitro embryo development noted during culture. Results Mean (+\\/- SD) age for study patients in this study group was 36.3 ± 4.0 (range = 28-45) yrs, and mean (+\\/- SD) terminal serum estradiol during IVF was 5929 +\\/- 4056 pmol\\/l. A moderate positive correlation (0.49; 95% CI 0.31 to 0.65) was noted between basal serum AMH and number of MII oocytes retrieved. Similarly, a moderate positive correlation (0.44) was observed between serum AMH and number of early cleavage-stage embryos (95% CI 0.24 to 0.61), suggesting a relationship between serum AMH and embryo development in IVF. Of note, serum AMH levels at baseline were significantly different for patients who did and did not undergo blastocyst transfer (15.6 vs. 10.9 pmol\\/l; p = 0.029). Conclusions While serum AMH has found increasing application as a predictor of ovarian reserve for patients prior to IVF, its roles to estimate in vitro embryo morphology and potential to advance to blastocyst stage have not been extensively investigated. These data suggest that baseline serum AMH determinations can help forecast blastocyst developmental during IVF. Serum AMH measured before treatment may assist patients, clinicians and embryologists as scheduling of embryo transfer is outlined. Additional studies are needed to confirm these correlations and to better define the role of baseline serum AMH level in the prediction of blastocyst formation.

  15. Assessment of the relationship of basal serum anti-mullerian hormone levels with oocyte quality and pregnancy outcomes in patients undergoing ICSI

    Gültekin Adanaş Aydın


    Full Text Available Background: Anti-Mullerian hormone (AMH is constantly secreted during menstrual cycles and may offer several advantages over traditional biomarkers of ovarian reserve. Objective: To assess the relationship of anti-Mullerian hormone (AMH values, which are used to evaluate ovary reserves, with oocyte and embryo quality and with ART outcomes in patients undergoing intra-cytoplasmic sperm injection (ICSI. Materials and Methods: This cross sectional study was performed using 50 women undergoing ICSI in IVF center of Zeynep Kamil Women's and Children's Hospital, İstanbul, Turkey. All patients received the long protocol. Follicle-stimulating hormone, luteinizing hormone, estradiol, and AMH levels were measured and antral follicle counts were obtained on the 3rd day of menstruation. A cut-off value based on the number of oocytes was determined for AMH, and women were evaluated after being divided into two groups as bad responders and good responders, according to their AMH levels. Results: Twelve (27.3% women were in bad responders group and 32 (72.7% women were in good responders group. AMH measurements were statistically significantly different between the two groups (p<0.01. Based on this significance, the researchers used ROC analysis to estimate a cut-off point for AMH. The researchers detected the good responders with an AMH level 1.90 or above, with 87.50% sensitivity, 66.67% specificity, 87.50% positive prediction, and 66.67% negative prediction (AUC=0.777, p<0.01. Conclusion: Basal AMH levels can be used as an indicator to determine the ovarian response in women undergoing ICSI. AMH can be used to predict the number of mature oocytes that can be collected during treatment and the number of oocytes that can be fertilized. However, AMH is not a valuable tool to evaluate oocyte quality, the development of high-quality embryos, or pregnancy conception.

  16. Evaluation of Ovarian Reserve by Measurement of the Serum Levels of Anti-Mullerian Hormone and Follicle-Stimulating Hormone in Intracytoplasmic Sperm Injection Cycles

    Roshan Nikbakht


    Full Text Available Background: It is important to evaluate ovarian reserves prior to intracytoplasmic sperm injection(ICSI treatment. The aim of this study is to determine the accuracy of anti-mullerian hormone(AMH as a marker for ovarian reserve and to compare it with day-3 serum follicle-stimulatinghormone (FSH levels.Materials and Methods: In this analytic, corss-sectional study, sequential sampling was done on70 infertile women who underwent ICSI treatment at Imam Khomeini Hospital, Ahvaz, Iran.Initially, 5cc of venous blood was drawn from each patient to measure serum AMH and FSH levelson the day-3 cycle.Patients were divided into two subgroups according to the numbers of oocytes retrieved. Patientswere classified as good responders if there were four or more oocytes retrieved, whereas patientswith less than four oocytes were poor responders.Results: The basal AMH level correlated with the number of oocytes retrieved (linear Pearsoncorrelation coefficient=0.599, however the basal FSH level had a weakly reverse correlation(correlation coefficient = -0.11.AMH levels had a sensitivity of 85% and specificity of 61.5%, with a cutoff value equal to 2.3 ng/mlwhich was higher than FSH.Conclusion: AMH serum levels are good predictors of ovarian reserve in comparison with FSH.

  17. Anti-Mullerian hormone serum values and ovarian reserve: can it predict a decrease in fertility after ovarian stimulation by ART cycles?

    Tito Silvio Patrelli

    Full Text Available BACKGROUND: A variety of indicators of potentially successful ovarian stimulation cycles are available, including biomarkers such as anti-Mullerian hormone. The aim of our study was to confirm the usefulness of serum anti-Mullerian hormone assay in predicting ovarian response and reproductive outcome in women eligible for ART cycles. MATERIALS: Forty-six women undergoing ART cycles at the Centre for Reproductive Medicine in Parma were recruited from March-to-June 2010. INCLUSION CRITERIA: age<42 years; body-mass-index = 20-25; regular menstrual cycles; basal serum FSH concentration <12 IU/L and basal serum estradiol concentration <70 pg/mL. The couples included in our study reported a variety of primary infertility causes. All women underwent FSH stimulation and pituitary suppression (GnRH-agonist/GnRH-antagonist protocols. Women were considered poor-responders if they had ≤ 3 oocytes; normal-responders 4-9 oocytes and high-responders ≥ 10 oocytes. Serum samples for the AMH assays were obtained on the first and last days of stimulation. A P value ≤ 0.05 was considered statistically significant. RESULT: FSH levels increased significantly when AMH levels decreased. The total dose of r-FSH administered to induce ovulation was not correlated to AMH. The number of follicles on the hCG, serum estradiol levels on the hCG-day, and the number of retrieved oocytes were significantly correlated to AMH. The number of fertilized oocytes was significantly correlated to the AMH levels. No significant correlation was found between obtained embryos or transferred embryos and AMH. Basal serum AMH levels were significantly higher than those measured on the hCG-day, which appeared significantly reduced. There was a significant correlation between AMH in normal responders and AMH in both high and poor responders. CONCLUSIONS: Our data confirm the clinical usefulness of AMH in ART-cycles to customize treatment protocols and suggest the necessity of verifying an

  18. Age-Specific Serum Anti-Mullerian Hormone and Follicle Stimulating Hormone Concentrations in Infertile Iranian Women

    Alireza Raeissi


    Full Text Available Background: Anti-Müllerian hormone (AMH is secreted by the granulosa cells of growing follicles during the primary to large antral follicle stages. Abnormal levels of AMH and follicle stimulating hormone (FSH may indicate a woman’s diminished ability or inability to conceive. Our aim is to investigate the changes in serum AMH and FSH concentrations at different age groups and its correlation with ovarian reserves in infertile women. Materials and Methods: This cross-sectional study analyzed serum AMH and FSH levels from 197 infertile women and 176 healthy controls, whose mean ages were 19-47 years. Sample collection was performed by random sampling and analyzed with SPSS version 16 software. Results: There were significantly lower mean serum AMH levels among infertile women compared to the control group. The mean AMH serum levels from different ages of infertile and control group (fertile women decreased with increasing age. However, this reduction was greater in the infertile group. The mean FSH serum levels of infertile women were significantly higher than the control group. Mean serum FSH levels consistently increased with increasing age in infertile women; however mean luteinizing hormone (LH levels were not consistent. Conclusion: We have observed increased FSH levels and decreased AMH levels with increasing age in women from 19 to 47 years of age. Assessments of AMH and FSH levels in combination with female age can help in predicting ovarian reserve in infertile women.

  19. Anti-Mullerian hormone levels decline under hormonal suppression: a prospective analysis in fertile women after delivery

    Ortner Iris


    Full Text Available Abstract Background AMH's reported stability during periods of hormonal change makes it a practical tool in assessing ovarian reserve. However, AMH declines with age and age-specific cut-offs remain to be established in women with proven fertility. This study aims to determine age-specific ranges of AMH in women with proven fertility. Methods Two hundred-ten fertile women, aged 18-40 years, were prospectively recruited for AMH measurements within 14 days after delivery and age stratified into 3 groups (18-30, 31-36 and 37-40 years. Eligibility required spontaneous conception within a maximal period of six months. Autoimmune diseases, chemotherapy, radiation, ovarian surgery and polycystic ovary syndrome precluded inclusion. Results 95% confidence intervals of AMH declined with advancing female age from 0.9-1.1 to 0.6-0.9 and 0.2-0.4 ng/mL (P Conclusions Like infertile populations, fertile women demonstrate declining AMH with advancing age. Uniformly lower levels than in infertile women suggest that AMH levels do not appear as stable under all hormonal influences as previously reported.

  20. The Correlations of Anti-Mullerian Hormone, Follicle-Stimulating Hormone and Antral Follicle Count in Different Age Groups of Infertile Women

    Ludmila Barbakadze


    Full Text Available Background: The objective of our study was to identify the correlations between the tests currently used in ovarian reserve assessment: anti-Mullerian hormone (AMH, follicle stimulating hormone (FSH and antral follicle count (AFC and to distinguish the most reliable markers for ovarian reserve in order to select an adequate strategy for the initial stages of infertility treatment. Materials and Methods: In this prospective study, 112 infertile women were assessed. Subjects were divided into three age groups: group I <35 years (n=39, group II 35-40 years (n=31, and group III 41-46 years (n=42. AMH, FSH and AFC were determined on days 2-3 of the patients’ menstrual cycles. Results: There was a significantly elevated negative correlation between age and AMH level (rs =-0.67, p<0.0001 and AFC (rs =-0.55, p<0.0001. We observed a significantly positive correlation between age and FSH (rs =0.38, p<0.0001. AMH negatively correlated with FSH (rs =-0.48, p<0.0001 and positively with AFC (r=- 0.71, p=0.0001. There was a moderate negative relation between FSH and AFC (r=-0.41, p=0.0001 and moderate positive relation between age and FSH (rs =0.38, p<0.0001. The correlation analysis performed in separate groups showed that AMH and AFC showed a statistically significant positive correlation for group I (r=0.57, p<0.0001, group II (r=0.69, p<0.0001 and group III (r=0.47, p<0.002. A statistically significant correlation between FSH and AMH was detected only in groups I (r=-0.41, p<0.02 and II (r=-0.55, p<0.0001. A statistically significant correlation existed between FSH and AFC only in group III (r=-0.42, p<0.006, as well as between age and AFC only in group I (r=-0.35, p<0.03. Conclusion: Currently, AMH should be considered as the more reliable of the ovarian reserve assessments tests compared to FSH. There is a strong positive correlation between serum AMH level and AFC. The use of AMH combined with AFC may improve ovarian reserve evaluation.

  1. Anti-mullerian hormone cut-off values for predicting poor ovarian response to exogenous ovarian stimulation in in-vitro fertilization

    Ruma Satwik


    Full Text Available Objectives: (a To establish the cut-off levels for anti-Mullerian hormone (AMH in a population of Indian women that would determine poor response. (b To determine which among the three ie.,: age, follicle stimulating hormone (FSH, or AMH, is the better determinant of ovarian reserve. Study Design: Prospective observational study. Setting: In vitro fertilization (IVF unit of a tertiary hospital. Materials and Methods: The inclusion criterion was all women who presented to the center for in-vitro fertilization/Intracytoplasmic sperm injection (IVF/ICSI. The exclusion criteria were age >45 years, major medical illnesses precluding IVF or pregnancy, FSH more than 20 IU/L, and failure to obtain consent. The interventions including baseline pelvic scan, day 2/3 FSH, luteinizing hormone (LH, estradiol estimations, and AMH measurement on any random day of cycle were done. Subjects underwent IVF according to long agonist or antagonist protocol regimen. Oocyte recovery was correlated with studied variables. The primary outcome measure was the number of oocytes aspirated (OCR. Three categories of ovarian response were defined: poor response, OCR ≤ 3; average response, OCR between 4 and 15; hyperresponse, OCR > 15. Results: Of the 198 patients enrolled, poor, average, and hyperresponse were observed in 23%, 63%, and 14% respectively. Correlation coefficient for AMH with ovarian response was r = 0.591. Area under the curve (AUCs for poor response for AMH, subject′s age, and FSH were 0.768, 0.624, and 0.635, respectively. The discriminatory level of AMH for prediction of absolute poor response was 2 pmoL/l, with 98% specificity and 20% sensitivity. Conclusions: AMH fares better than age and FSH in predicting the overall ovarian response and poor response, though it cannot be the absolute predictor of non-responder status. A level of 2 pmol/l is discriminatory for poor response.

  2. Up-regulation of SOX9 in sertoli cells from testiculopathic patients accounts for increasing anti-mullerian hormone expression via impaired androgen receptor signaling.

    Kuo-Chung Lan

    Full Text Available BACKGROUND: Testosterone provokes Sertoli cell maturation and represses AMH production. In adult patients with Sertoli-cells-only syndrome (SCOS and androgen insensitivity syndrome (AIS, high level of AMH expression is detected in Sertoli cells due to defect of androgen/AR signaling. OBJECTIVE: We postulated that up-regulation of SOX9 due to impairment of androgen/AR signaling in Sertoli cells might explain why high level of anti-Mullerian hormone (AMH expression occur in these testiculopathic patients. METHODS: Biological research of testicular specimens from men with azoospermia or mouse. The serum hormone levels were studied in 23 men with obstructive azoospermia, 33 men with SCOS azoospermia and 21 volunteers with normal seminograms during a period of 4 years. Immunohistochemical staining and reverse-transcription PCR were used to examine the relationships among AR, SOX9 and AMH expression in adult human and mouse testes. The ability of AR to repress the expression of SOX9 and AMH was evaluated in vitro in TM4 Sertoli cells and C3H10T1/2 cells. RESULTS: SCOS specimens showed up-regulation of SOX9 and AMH proteins but down-regulation of AR proteins in Sertoli cells. The mRNA levels of AR were significantly lower and the SOX9, AMH mRNA levels higher in all SCOS patients compared to controls (P< 0.05. The testosterone levels in the SCOS patients were within the normal range, but most were below the median of the controls. Furthermore, our in vitro cell line experiments demonstrated that androgen/AR signaling suppressed the gene and protein levels of AMH via repression of SOX9. CONCLUSIONS: Our data show that the functional androgen/AR signaling to repress SOX9 and AMH expression is essential for Sertoli cell maturation. Impairment of androgen/AR signaling promotes SOX9-mediated AMH production, accounts for impairments of Sertoli cells in SCOS azoospermic patients.

  3. Follicular-fluid anti-Mullerian hormone (FF AMH is a plausible biochemical indicator of functional viability of oocyte in conventional in vitro fertilization (IVF cycles

    Bindu N Mehta


    Full Text Available Context: Oocyte quality may be a governing factor in influencing in vitro fertilization (IVF outcomes. However, morphological evaluation of oocyte quality is difficult in conventional IVF cycles. Follicular-fluid (FF, the site for oocyte growth and development, has not yet been sufficiently explored to obtain a marker indicative of oocyte quality. Anti-Mullerian hormone (AMH is produced by granulosa cells of preantral and early-antral follicles and is released in FF. Aim: To investigate AMH as a biochemical indicator of functional viability/quality of oocyte produced in the FF micro-environmental milieu. Settings and Design: Prospective study involving 132 cycles of conventional IVF-embryo transfer (ET in infertile women. Subjects and Methods: AMH concentration was estimated in pooled FF on day of oocyte pickup. Cycles were sorted into low and high groups according to median (50 th centile values of measurement. Main outcome measure was oocyte viability, which included morphological assessment of oocyte quality, fertilization rate, clinical pregnancy, and implantation rates. Statistical Analysis: Graph-pad Prism 5 statistical package. Results: Low FF AMH group shows significantly higher percentage of top-quality oocytes (65.08 ± 24.88 vs. 50.18 ± 25.01%, P =0.0126, fertilization (83.65 ± 18.38 vs. 75.78 ± 21.02%, P =0.0171, clinical pregnancy (57.57 vs. 16.67%, P <0.0001, and embryo implantation rates (29.79 vs. 7.69%, P <0.0001 compared to high FF AMH group. FF AMH shares an inverse correlation with FF E2 (Pearson r = −0.43, r 2 = 0.18 and clinical pregnancy (Pearson r = −0.46, r 2 = 0.21. Threshold value of FF AMH for pregnancy is <1.750 ng/mg protein. Conclusion: FF AMH is a plausible biochemical indicator of functional viability of oocyte in conventional IVF cycles.

  4. Role of baseline antral follicle count and anti-Mullerian hormone in prediction of cumulative live birth in the first in vitro fertilisation cycle: a retrospective cohort analysis.

    Hang Wun Raymond Li

    Full Text Available OBJECTIVE: This retrospective study determined for the first time the role of baseline antral follicle count (AFC and serum anti-Mullerian hormone (AMH level in the first in-vitro fertilisation (IVF cycle in predicting cumulative live birth from one stimulation cycle. METHODS: We studied 1,156 women (median age 35 years undergoing the first IVF cycle. Baseline AFC and AMH level on the day before ovarian stimulation were analysed. The main outcome measure was cumulative live birth in the fresh plus all the frozen embryo transfers after the same stimulation cycle. RESULTS: Serum AMH was significantly correlated with AFC. Both AMH and AFC showed significant correlation with age and ovarian response in the stimulated cycle and total number of transferrable embryos. Baseline AFC and serum AMH were significantly higher in subjects attaining a live birth than those who did not in the fresh stimulated cycle, as well as those attaining cumulative live birth. There was a significant trend of higher cumulative live birth rate in women with higher AMH or AFC. However, logistic regression revealed that both AMH and AFC were not significant predictors of cumulative live birth after adjusting for age and number of embryos available for transfer. Considering only one single predictor, the areas under the ROC curves for AMH (0.646, 95% CI 0.616-0.675 and age (0.648, 95% CI 0.618-0.677 were slightly higher than that for AFC (0.617, 95% CI 0.587-0.647 in predicting cumulative live birth. However, a model combining AMH (with or without AFC and age of the women only classified an addition of less than 2% of subjects correctly compared to the model with age alone. CONCLUSION: Baseline AFC and serum AMH have only modest predictive performance on the occurrence of cumulative live birth, and may not give additional value on top of the women's age.

  5. Comparing serum basal and follicular fluid levels of anti-Mullerian hormone as a predictor of in vitro fertilization outcomes in patients with and without polycystic ovary syndrome

    The prediction of in vitro fertilization (IVF) outcomes by anti-Mullerian hormone (AMH) measurement is getting increasing attention from clinicians. This study compares the relationship between serum or intrafollicular AMH levels and IVF outcomes in women with and without polycystic ovary syndrome (PCOS).This prospective study was carried out in two university-based fertility clinics. Serum samples were collected on cycle day 3 and follicular fluid (FF) was collected on the day of oocyte retrieval from 26 women with PCOS and 42 normo-ovulatory controls. AMH levels were measured in the samples using immunoenzymatic assay. The relationship between serum or FF AMH levels and IVF outcomes, including number of oocytes retrieved, oocyte maturation rate, fertilization rate, implantation rate, high quality grade embryo rate, and biochemical and clinical pregnancy rates were further assessed.Median serum basal AMH and FF AMH levels were significantly higher in the PCOS group as compared to controls, the values being 14.2 ng/mL vs. 3.2 ng/mL (P<.001) and 8.2 ng/g protein vs. 4.7 ng/g protein (P<.01), respectively. In both groups, serum basal AMH levels showed a positive correlation with number of oocytes retrieved (r=0.323; P=.037 in control vs. r=0.529; P=.005 in PCOS). In the control group, there was a positive relationship between serum basal AMH levels and percentage of matured oocytes (r = 0.331; P=.032) and implantation rate (r=0.305; P=.05).Serum basal, and not intrafollicular, AMH levels may be a good predictive factor for quantitative and qualitative IVF outcomes in normo-ovulatory, but not in PCOS patients (Author).

  6. Distribution and responsiveness of rat anti-Mullerian hormone during ovarian development and VCD-induced ovotoxicity

    Mark-Kappeler, Connie J.; Sen, Nivedita; Keating, Aileen F.; Sipes, I. Glenn; Hoyer, Patricia B.


    Anti-Müllerian hormone (AMH) is produced by granulosa cells in primary to small antral follicles of the adult ovary and helps maintain primordial follicles in a dormant state. The industrial chemical, 4-vinylcyclohexene diepoxide (VCD) causes specific ovotoxicity in primordial and small primary follicles of mice and rats. Previous studies suggest that this ovotoxicity involves acceleration of primordial to primary follicle recruitment via interactions with the Kit/Kit ligand signaling pathway...

  7. The circadian variation in Anti-Mullerian hormone in patients with polycystic ovary syndrome differs significantly from normally ovulating women.

    Leif Bungum

    Full Text Available OBJECTIVE: [corrected] To improve the biologic understanding of the Polycystic Ovarian Syndrome (PCOS condition by examining the circadian variation and relationship between Anti Müllerian Hormone (AMH, gonadotropins and ovarian steroids in PCOS patients compared to normally ovulating and menstruating women. By comparing the pattern of co-variation between AMH and Luteinizing Hormone, two compounds closely linked to hyperandrogenism and anovulation in PCOS, the involvement of the Hypothalamic-Pituitary-Ovarian axis in PCOS pathology could be elucidated. PATIENTS: Eight normal-weighted young, anovulatory PCOS-women as study group and ten normal menstruating and ovulating women as controls. INTERVENTIONS: Observational prospective study of the circadian variation in AMH, gonadotropins, sex steroids and androgens in a study and a control group. A circadian profile was performed in each study and control subject during a 24-h period by blood sampling every second hour, starting at 8:00 a.m. and continuing until 8:00 a.m. the following day. RESULTS: Significant differences in hormonal levels were found between the groups, with higher concentrations of AMH, LH and androgens in the PCOS group and lower amounts of FSH and progesterone. A distinct difference in the circadian variation pattern of AMH and LH between PCOS patients and normal controls was seen, with PCOS patients presenting a uniform pattern in serum levels of AMH and LH throughout the study period, without significant nadir late-night values as was seen in the control group. In PCOS women, a significant positive association between LH/ FSH and testosterone was found opposite to controls. MAIN OUTCOME MEASURES: Circadian variation in Anti-Müllerian Hormone, gonadotropins and ovarian steroids and the covariation between them. CONCLUSION: A significant difference in the circadian secretion of LH and AMH in PCOS women compared to normally ovulating women indicate an increased GnRH pulse

  8. Cut-Off Levels of Anti-Mullerian Hormone for The Prediction of Ovarian Response, In Vitro Fertilization Outcome and Ovarian Hyperstimulation Syndrome

    Ali Salmassi


    Full Text Available Background: Evaluation of anti-mullerian hormone (AMH cut-off levels in assisted reproductive technology (ART as predictive factor for individualization of stimulation protocols and to avoid ovarian hyperstimulation syndrome (OHSS. Materials and Methods: In a retrospective study, 177 infertile patients were assessed for AMH in serum and follicular fluid (FF on the day of follicular puncture (FP, between 2012 and 2013 in Kiel, Germany. AMH levels and pregnancy rates were compared between low, moderate and high responders and cut-off levels of low and high responders. AMH cut-off levels in pathological cases were evaluated in analysis 1 (OHSS and in analysis 2 [polycystic ovarian syndrome, (PCOS] and compared in analysis 3 to normal endocrinological parameters. Results: AMH levels in FF were higher than in serum (P<0.001. AMH levels in serum and FF increased from low through moderate to high responders (P<0.001. Pregnancy rates were 14.7, 23.3 and 44.9% (P=0.009, respectively. AMH cut-off level for poor responders was 0.61 ng/ml in serum with a pregnancy rate of 13.8 and 37.1% for below and above of this level, respectively. For FF, it was 1.43 ng/ml. AMH levels in analysis 1 and 2 were significantly higher than in analysis 3 (P=0.001. AMH cut-off level for OHSS was 1.5 ng/ml in serum with OHSS rates of 80.8 and 19.2 % for above and below of the level, respectively. For FF, it was 2.7 ng/ml. PCOS patients had an AMH cut-off level of 3.9 ng/ml in serum and 6.8 ng/ml in FF, resulting in a PCOS rate of 100% above this level. Conclusion: AMH levels can help to assess ovarian response potential and guide ovarian stimulation while avoiding OHSS.

  9. Maternal menopause as a predictor of anti-Mullerian hormone level and antral follicle count in daughters during reproductive age

    Bentzen, J G; Forman, J L; Larsen, E C;


    STUDY QUESTION: Is the ovarian reserve in a woman at a given age associated with her mother's age at menopause? SUMMARY ANSWER: We demonstrated a significant, positive association between age at maternal menopause and serum anti-Müllerian hormone (AMH) levels and antral follicle count (AFC) in...... daughters. The rate of decline in serum-AMH level and AFC is also associated with age at maternal menopause. WHAT IS KNOWN AND WHAT THIS PAPER ADDS: The association between menopausal age in mothers and daughters has been established through several epidemiological studies. This paper shows that early...... prospective cohort whose mothers' age at natural menopause was known. PARTICIPANTS, SETTING AND METHODS: Participants were recruited from female health care workers aged 20-40 years employed at Copenhagen University Hospital, Rigshospitalet, and were enrolled in the study between September 2008 and February...

  10. Serum and follicular anti-Mullerian hormone levels in women with polycystic ovary syndrome (PCOS under metformin

    Falbo Angela


    Full Text Available Abstract Background No data regarding metformin effects on follicular fluid anti-Müllerian hormone (AMH levels were to date available in literature. The aim of the present study was to evaluate in patients with polycystic ovary syndrome (PCOS whether metformin administration affects serum and follicular AMH levels, and whether this is related to ovarian response to the treatment. Methods Twenty young patients with PCOS who had received metformin were enrolled. Ten patients were anovulatory (Met-anov group, whereas the other 10 were ovulatory (Met-ov group but had failed to conceive. Further untreated PCOS (PCOS controls, n. 10 and healthy controls (non-PCOS controls, n. 10 who were scheduled for laparoscopic surgery were enrolled. In each subjects, clinical and biochemical evaluations were performed. AMH concentrations in blood and antral follicular fluid were assayed. Results In both Met-anov and Met-ov groups, and without difference between them, serum androgens and AMH, and indices of insulin resistance were significantly (p p Conclusions Metformin administration in patients with PCOS exerts a differential action on the ovarian AMH levels on the basis of ovulatory response. Changes in AMH levels in antral follicular fluid during metformin treatment could be involved in the local mechanisms mediating the ovulatory restoration.

  11. Prediction of reproductive outcomes according to different serum anti-Mullerian hormone levels in females undergoing intracystoplasmic sperm injection.

    Santiago Brugo Olmedo

    Full Text Available BACKGROUND AND AIM OF THE STUDY: Serum anti-Müllerian hormone (AMH is a reliable marker of ovarian reserve, and it has been shown to be correlated with reproductive outcomes in grouped analyses. However, practical data is scarce for the physician and the patients to predict these outcomes in an individual couple according to serum AMH measured prior to assisted reproduction technology (ART procedures. STUDY DESIGN: To address this question, we performed an analytic observational study including 145 females undergoing intracytoplasmic sperm injection (ICSI in a single center. Results were analyzed according to serum AMH; subgroup analyses were performed by grouping patients according to patient's age and FSH levels. RESULTS: The risk of cycle cancellation decreased from 64% in patients with serum AMH ≤ 3 pmol/L (0.42 ng/mL to 21% with AMH ≥ 15 pmol/L (2.10 ng/mL. Cycle cancellation occurred in approximately two-thirds of the patients with AMH ≤ 3 pmol/L irrespective of the FSH level. However, with higher AMH values the risk of cycle cancellation decreased more significantly in patients with normal FSH. The rate of good response increased from almost null in patients with AMH ≤ 3 pmol/L to 61% in those with AMH ≥ 15 pmol/L. The positive correlation between good response and AMH was also significant, but with lower absolute rates, when patients were grouped according to their age or FSH levels. Pregnancy rate increased moderately, but significantly, from 31% with AMH ≤ 3 pmol/L to 35% with AMH ≥ 15 pmol/L. CONCLUSIONS: We provide estimates of reproductive outcomes according to individualized values of serum AMH, in general and in subgroups according to patient's age or serum FSH, which are helpful for the clinician and the couple in their decision making about starting an assisted reproductive treatment.

  12. Uso clínico do hormônio antimülleriano em ginecologia Clinical use for anti-mullerian hormone in gynecology

    Gustavo Salata Romão


    Full Text Available O hormônio antimülleriano (AMH é uma glicoproteína produzida pelas células granulosas de folículos ovarianos primários, pré-antrais e pequenos folículos antrais e ultimamente sua aplicabilidade clínica tem sido demonstrada através de diversos estudos. A predição da resposta à estimulação ovariana para fertilização in vitro corresponde a sua mais frequente utilização na prática clínica, sendo rotineiramente realizada em muitos serviços para identificar subgrupos de mulheres suscetíveis a má resposta ou a Síndrome da Hiperestimulação Ovariana. Existem perspectivas de que o AMH possa ser aplicável na individualização do risco de injúria gonadal iatrogênica em mulheres portadoras de neoplasia que serão submetidas a quimioterapia. Também é provável que as dosagens de AMH sejam incluídas nos protocolos de investigação de amenorreias e oligomenorreias, uma vez que seus níveis encontram-se elevados em pacientes portadoras da Síndrome dos Ovários Policísticos, reduzidos em casos de falência ovariana prematura e normais em outras condições como a hiperprolactinemia e o hipogonadismo hipogonadotrófico. É possível que futuramente o AMH venha a ser utilizado na predição da idade de menopausa e do prognóstico reprodutivo da mulher, fornecendo bases sólidas ao aconselhamento conceptivo e contraceptivo.Anti-mullerian hormone (AMH is a glycoprotein produced by granulosa cells of primary, pre-antral and small antral ovarian follicles and its clinical applicability has been recently demonstrated by several studies. Prediction of the response to ovarian stimulation for in vitro fertilization corresponds to the most frequent utilization of AMH in clinical practice, being routinely assessed in many services to identify subgroups of women susceptible to a poor response or to Ovarian Hyperstimulation Syndrome. There are great perspectives that AMH may be applicable to the individual determination of risk for

  13. Changes in Anti-Mullerian Hormone (AMH) throughout the Life Span: A Population-Based Study of 1027 Healthy Males from Birth (Cord Blood) to the Age of 69 Years

    Aksglæde, Lise; Sørensen, K; Boas, M.;


    Context: Anti-Mullerian hormone (AMH), which is secreted by immature Sertoli cells, triggers the involution of the fetal Mullerian ducts. AMH is a testis-specific marker used for diagnosis in infants with ambiguous genitalia or bilateral cryptorchidism. Aim: The aim of the study was to describe the...... at 3 and 12 months), n = 55] and another group through puberty [(biannual measurements), n = 83]. Main Outcome Measures: Serum AMH was determined by a sensitive immunoassay. Serum testosterone, LH, and FSH were measured, and pubertal staging was performed in boys aged 6 to 20 yr (n = 616). Results......: Serum AMH was above the detection limit in all samples with a marked variation according to age and pubertal status. The median AMH level in cord blood was 148 pmol/liter and increased significantly to the highest observed levels at 3 months (P <0.0001). AMH declined at 12 months (P <0.0001) and...

  14. Changes in Anti-Mullerian Hormone (AMH) throughout the Life Span: A Population-Based Study of 1027 Healthy Males from Birth (Cord Blood) to the Age of 69 Years

    Aksglæde, Lise; Sørensen, K; Boas, M.;


    Context: Anti-Mullerian hormone (AMH), which is secreted by immature Sertoli cells, triggers the involution of the fetal Mullerian ducts. AMH is a testis-specific marker used for diagnosis in infants with ambiguous genitalia or bilateral cryptorchidism. Aim: The aim of the study was to describe...... the ontogeny of AMH secretion through life in healthy males. Setting:This was a population-based study of healthy volunteers. Participants: Participants included 1027 healthy males from birth (cord blood) to 69 yr. A subgroup was followed up longitudinally through the infantile minipuberty [(in cord blood......, and at 3 and 12 months), n = 55] and another group through puberty [(biannual measurements), n = 83]. Main Outcome Measures: Serum AMH was determined by a sensitive immunoassay. Serum testosterone, LH, and FSH were measured, and pubertal staging was performed in boys aged 6 to 20 yr (n = 616). Results...

  15. 抗苗勒氏管激素与多囊卵巢综合症治疗效果的相关性%Correlation of anti-mullerian hormone and the effect of polycystic ovary syndrome treatment

    武雁; 宋晖; 田国华; 滑玮


    目的:研究多囊卵巢综合症治疗效果和抗苗勒氏管激素(Anti-Mullerian hormone,AMH)的相关性.方法:收集我院门诊2015年11月至2016年2月多囊卵巢综合征不孕症患者,排除其他因素后纳入140例.根据患者空腹血糖、空腹胰岛素和雄激素值分组:对照组:空腹血糖、空腹胰岛素和雄激素正常;糖代谢异常组:空腹血糖或/和空腹胰岛素升高,雄激素正常;高雄激素组:雄激素升高,空腹血糖或/和空腹胰岛素正常;糖代谢异常合并高雄激素组:空腹血糖或/和空腹胰岛素升高和雄激素升高的.给予达英-35或/和二甲双胍治疗后比较各组治疗前后和组间的抗苗勒氏管激素(Anti-Mullerian hormone,AMH)、空腹血糖、空腹胰岛素、胰岛素抵抗指数和雄激素变化.结果:糖代谢异常组,高雄激素组和糖代谢异常合并高雄激素组的AMH值,明显高于对照组(P<0.05).其中糖代谢异常合并高雄激素组的AMH值明显高于糖代谢异常组及高雄激素组(P<0.05).药物治疗后各组的AMH值比未治疗前均有明显下降(P<0.05).结论:AMH、糖代谢和雄激素三者可能具有互相影响、互相作用的关系.血清AMH水平为诊断和研究PCOS提供了一个重要的突破点.




    Full Text Available ABSTRACT:: A number off ovariian reserve ttestts are beiing used tto dettermiine oocytte reserve tto hellp prediictt iin viittro fferttiilliizattiion outtcome.. Thiis sttudy was underttaken tto ffiind iiff any correllattiion exiistts bettween llevells off Anttii--Mulllleriian (AMH,, ffolllliiclle sttiimullattiing hormone (FSH and esttradiioll (Ett,, wiitth tthe ovariian reserve.. Purposiive samplliing was underttaken.. IInfferttiilliitty pattiientts seekiing ttreattmentt att JJaiipur Ferttiilliitty and Mediicall Research Centtre,, a tterttiiary care uniitt off Mahattma Gandhii Mediicall Collllege and Hospiittall ffrom May--Dec2011 were iinclluded iin tthe sttudy.. IIn allll 105 pattiientts were sttudiied.. Serum llevells off hormones were dettermiined att day 3 and ovariian ffolllliiclle response was assessed on day off admiiniisttrattiion off HCG.. Resulltts revealled tthatt Serum AMH was siigniiffiicanttlly correllatted (r 0..302 p0..1 and Ett (r 0..999 p>0..1 were nott ffound tto have any such associiattiion wiitth ovariian reserve iin tthe sttudy.. KEY WORDS:: ovariian reserve,, AMH,, FSH,, esttradiioll,, IIVF.

  17. Ovarian response and cumulative live birth rate of women undergoing in-vitro fertilisation who had discordant anti-Mullerian hormone and antral follicle count measurements: a retrospective study.

    Hang Wun Raymond Li

    Full Text Available OBJECTIVE: To evaluate ovarian response and cumulative live birth rate of women undergoing in-vitro fertilization (IVF treatment who had discordant baseline serum anti-Mullerian hormone (AMH level and antral follicle count (AFC. METHODS: This is a retrospective cohort study on 1,046 women undergoing the first IVF cycle in Queen Mary Hospital, Hong Kong. Subjects receiving standard IVF treatment with the GnRH agonist long protocol were classified according to their quartiles of baseline AMH and AFC measurements after GnRH agonist down-regulation and before commencing ovarian stimulation. The number of retrieved oocytes, ovarian sensitivity index (OSI and cumulative live-birth rate for each classification category were compared. RESULTS: Among our studied subjects, 32.2% were discordant in their AMH and AFC quartiles. Among them, those having higher AMH within the same AFC quartile had higher number of retrieved oocytes and cumulative live-birth rate. Subjects discordant in AMH and AFC had intermediate OSI which differed significantly compared to those concordant in AMH and AFC on either end. OSI of those discordant in AMH and AFC did not differ significantly whether either AMH or AFC quartile was higher than the other. CONCLUSIONS: When AMH and AFC are discordant, the ovarian responsiveness is intermediate between that when both are concordant on either end. Women having higher AMH within the same AFC quartile had higher number of retrieved oocytes and cumulative live-birth rate.

  18. 抗苗勒管激素在卵巢功能及相关疾病中的应用进展%Function of Anti-Mullerian Hormone in Ovary-related Diseases



    抗苗勒管激素(anti-Mullerian hormone,AMH)是睾丸支持细胞和卵巢颗粒细胞分泌的一种由二硫键连接两个相同亚基的二聚体糖蛋白,属于转化生长因子β超家族成员,具有调节两性生殖细胞发育的功能.在卵巢功能方面,AM能够调节卵泡发育,参与卵泡生长过程,还能早期评估卵巢储备情况,及早预测绝经年龄,更能早期发现卵巢疾病并评估疾病发展及预后,在辅助生殖技术中亦起到重要作用.随着检测技术的发展,AMH浓度的测量更加简便和精确,而且与其他生物学指标比较,AMH浓度相对稳定,所以AMH已成为近年来的研究热点之一.就近年来AMH与卵巢功能及相关疾病的关系综述如下.

  19. 抗苗勒氏管激素在多囊卵巢综合征诊断中的价值%The value of anti-mullerian hormone in the diagnosis of polycystic ovarian syndrome

    刘俊芬; 程丽平


    目的:探讨抗苗勒氏管激素(Anti-Mullerian hormone,AMH)在多囊卵巢综合征(PCOS)中的诊断价值.方法:用酶联免疫吸附法(ELISA)测定56例PCOS患者血清AMH浓度,并且与30例正常妇女进行对照;以AMH 5.0ng/ml为界值,将其与PCOS的诊断标准进行对比,敏感度为89.29%,特异度为70.00%.结果:PCOS组与对照组血清AMH的浓度比较有显著性差异;在患病率较高的PCOS不孕诊断中,AMH检测是一项较好的指标.结论:在精确超声数据得不到的情况下,血清AMH浓度能够代替卵巢窦卵泡数目,是替代反映窦卵泡计数的理想指标.

  20. Follicle stimulating hormone and anti-Mullerian hormone per oocyte in predicting in vitro fertilization pregnancy in high responders: a cohort study.

    Andrea Weghofer

    Full Text Available BACKGROUND: Follicle stimulating hormone (FSH and Anti-Müllerian hormone (AMH are utilized to differentiate between good and poor response to controlled ovarian hyperstimulation. Their respective roles in defining functional ovarian reserve remain, however, to be elucidated. To better understand those we investigated AMH and FSH per oocyte retrieved (AMHo and FSHo. METHODOLOGY/PRINCIPAL FINDINGS: Three-hundred and ninety-six women, undergoing first in vitro fertilization cycles, were retrospectively evaluated. Women with oocyte yields >75(th percentile for their age group were identified as high responders. In a series of logistic regression analyses, AMHo and FSHo levels were then evaluated as predictive factors for pregnancy potential in high responders. Patients presented with a mean age of 38.0±5.0 years, mean baseline FSH of 11.8±8.7 mIU/mL and mean AMH of 1.6±2.1 ng/mL. Those 88 women, who qualified as high responders, showed mean FSH of 9.7±6.5 mIU/mL, AMH of 3.1±3.1 ng/mL and oocyte yields of 15.8±7.1. Baseline FSH and AMH did not predict pregnancy in high responders. However, a statistically significant association between FSHo and pregnancy was observed in high responders, both after univariate regression (p = 0.02 and when adjusted for age, percentage of usable embryos, and number of embryos transferred (p = 0.03. Rate of useable embryos also significantly affected pregnancy outcome independently of FSHo (p = 0.01. AMHo was also associated with clinical pregnancy chances in high responders (p = 0.03 and remained significant when adjusted for usable embryos and number of embryos transferred (p = 0.04. CONCLUSIONS: AMHo and FSHo are predictive of pregnancy potential in high responders, but likely reflect different responsibilities in recruitment and maturation of growing follicle cohorts.

  1. Body Mass Index Is Associated with Impaired Semen Characteristics and Reduced Levels of Anti-Mullerian Hormone across a Wide Weight Range.

    Jorunn M Andersen

    Full Text Available There is still controversy as to how body mass index (BMI affects male reproduction. We investigated how BMI is associated with semen quality and reproductive hormones in 166 men, including 38 severely obese men. Standard semen analysis and sperm DNA integrity analysis were performed, and blood samples were analysed for reproductive hormones. Adjusted for age and time of abstinence, BMI was negatively associated with sperm concentration (B = -0.088, P = 0.009, total sperm count (B = -0.223, P = 0.001, progressive sperm motility (B = -0.675, P = 0.007, normal sperm morphology (B = -0.078, P = 0.001, and percentage of vital spermatozoa (B = -0.006, P = 0.027. A negative relationship was observed between BMI and total testosterone (B = -0.378, P < 0.001, sex hormone binding globulin (B = -0.572, P < 0.001, inhibin B (B = -3.120, P < 0.001 and anti-Müllerian hormone (AMH (B = -0.009, P < 0.001. Our findings suggest that high BMI is negatively associated with semen characteristics and serum levels of AMH.

  2. Circadian variation in concentration of anti-Mullerian hormone in regularly menstruating females: relation to age, gonadotrophin and sex steroid levels

    Bungum, Leif; Jacobsson, Anna-Karin; Rosén, Fredrik;


    Anti-Müllerian hormone (AMH) is a promising marker of ovarian reserve. The aim of the study is to assess the circadian variation in AMH, and to evaluate its clinical relevance and biological aspects as an effect of age and other endocrine mechanisms involved in the regulation of AMH secretion....

  3. Cloning and expression of anti-mullerian hormone partial gene and preparation of polyclonal antibody%罗非鱼AMH基因的原核表达及多克隆抗体制备

    丁炜东; 曹哲明; 曹丽萍


    抗苗勒氏管激素(anti-mullerian hormone,AMH),也称苗勒氏管抑制物质(mullerian inhibiting substance,MIS),为肽类生长因子,属于TGF-β生长和分化因子家族.为研究AMH 对奥利亚罗非鱼性腺发育的作用,应用DNAstar软件分析罗非鱼AMH基因的抗原性,选择抗原性较强的22~243氨基酸作为目的片段构建了AMH的原核表达载体并进行融合表达.首先利用RT-PCR方法从性腺中扩增出长约663 bp的目的序列AMH基因,克隆至T载体中,经酶切鉴定和序列测定分析确认序列的正确性后将此片段克隆到表达载体pGEX-5x-1中构建重组表达质粒pGEX-AMH,并在大肠杆菌BL21中获得了高表达,目的蛋白约占菌体总蛋白的38.7%.菌体经溶菌酶裂解,制备无细胞抽提液,GSTrap FF column柱层析后得到分子量为49 ku单一条带的目的蛋白.目的蛋白经FactorXa酶切裂解,GSTrap FF column过柱纯化后得到纯化的AMH蛋白,分子量为26 ku,浓度为2.6 mg/mL.以每只20μg的剂量4次免疫ICR小鼠,免疫小鼠可以检测到特异性针对AMH蛋白的血清抗体应答,免疫组抗体水平显著高于空白组(P<0.05),且加强免疫第5周后抗体效价为0.672±0.411,达到高峰值,血清效价为1∶2 000.试验结果表明表达产物具有免疫原性,可以刺激机体产生免疫应答.

  4. 抗苗勒管激素用于多囊卵巢综合征诊断的探讨%Study of Sersum Anti-mullerian Hormone as Marker for Diagnosis of Polycystic Ovary Syndrome

    张芳; 王建辉


    目的:探讨抗苗勒管激素(AMH)在多囊卵巢综合征(PCOS)诊断的应用价值.方法:用电化学免疫发光法检测75例PCOS患者(研究组)及86例正常排卵育龄妇女(对照组)月经第2日内分泌水平,酶联免疫吸附法(ELISA)检测血清AMH,同时应用超声诊断仪行卵巢卵泡计数(FN).结果:①研究组与对照组血清AMH水平分别为(8.21±2.32) mg/L和(2.39±1.29) mg/L,研究组AMH水平明显高于对照组.②研究组AMH与FN、睾酮(T)、黄体生成激素(LH)、LH/FSH呈正相关(r分别为0.489,0.215,0.312和0.256,均P<0.05),而与卵泡刺激素(FSH)、雌二醇(E2)、泌乳素(PRL)、体质量指数(BMI)无线性相关性(r分别为0.12,0.19,0.10和0.16,均P>0.05).结论:AMH用于临床PCOS诊断有一定价值,且简便易行.%Objective:To explore whether anti-mullerian hormone (AMH) measurement in the serum could be a marker for diagnosis of polycystic ovary syndrome (PCOS). Methods: Serum AMH was measured with immunoassay in a cohort of 75 PCOS patients and 86 controls,and follicle number was estimated by ultrasound scanning. Results:Serum AMH levels were 4-fold higher in PCOS patients than in control group [ (8.21±2.32) mg/L vs. (2.39±1.29) mg/L] ,and were significantly related to the follicle number in the two groups. In PCOS patients, AMH concentrations correlated with the number of ovarian follicles,testosterone 、LH and LH/FSH(r= 0.489,0.215,0.312 and 0.256,P0.05).ConcIusions:The serum AMH level was a marker which may be used for diagnosis of the PCOS.

  5. A Tandem Duplicate of Anti-Mullerian Hormone with a Missense SNP on the Y Chromosome Is Essential for Male Sex Determination in Nile Tilapia, Oreochromis niloticus.

    Minghui Li


    Full Text Available Variation in the TGF-β signaling pathway is emerging as an important mechanism by which gonadal sex determination is controlled in teleosts. Here we show that amhy, a Y-specific duplicate of the anti-Müllerian hormone (amh gene, induces male sex determination in Nile tilapia. amhy is a tandem duplicate located immediately downstream of amhΔ-y on the Y chromosome. The coding sequence of amhy was identical to the X-linked amh (amh except a missense SNP (C/T which changes an amino acid (Ser/Leu92 in the N-terminal region. amhy lacks 5608 bp of promoter sequence that is found in the X-linked amh homolog. The amhΔ-y contains several insertions and deletions in the promoter region, and even a 5 bp insertion in exonVI that results in a premature stop codon and thus a truncated protein product lacking the TGF-β binding domain. Both amhy and amhΔ-y expression is restricted to XY gonads from 5 days after hatching (dah onwards. CRISPR/Cas9 knockout of amhy in XY fish resulted in male to female sex reversal, while mutation of amhΔ-y alone could not. In contrast, overexpression of Amhy in XX fish, using a fosmid transgene that carries the amhy/amhΔ-y haplotype or a vector containing amhy ORF under the control of CMV promoter, resulted in female to male sex reversal, while overexpression of AmhΔ-y alone in XX fish could not. Knockout of the anti-Müllerian hormone receptor type II (amhrII in XY fish also resulted in 100% complete male to female sex reversal. Taken together, these results strongly suggest that the duplicated amhy with a missense SNP is the candidate sex determining gene and amhy/amhrII signal is essential for male sex determination in Nile tilapia. These findings highlight the conserved roles of TGF-β signaling pathway in fish sex determination.

  6. FOXL2 Is an Essential Activator of SF-1-Induced Transcriptional Regulation of Anti-Mullerian Hormone in Human Granulosa Cells.

    Hanyong Jin

    Full Text Available Anti-Müllerian hormone (AMH is required for proper sexual differentiation by regulating the regression of the Müllerian ducts in males. Recent studies indicate that AMH could be an important factor for maintaining the ovarian reserve. However, the mechanisms of AMH regulation in the ovary are largely unknown. Here, we provide evidence that AMH is an ovarian target gene of steroidogenic factor-1 (SF-1, an orphan nuclear receptor required for proper follicle development. FOXL2 is an evolutionally conserved transcription factor, and its mutations cause blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES, wherein affected females display eyelid defects and premature ovarian failure (POF. Notably, we found that functional FOXL2 is essential for SF-1-induced AMH regulation, via protein-protein interactions between FOXL2 and SF-1. A BPES-inducing mutant of FOXL2 (290-291delCA was unable to interact with SF-1 and failed to mediate the association between SF-1 and the AMH promoter. Therefore, this study identified a novel regulatory circuit for ovarian AMH production; specifically, through the coordinated interplay between FOXL2 and SF-1 that could control ovarian follicle development.

  7. [Anti mullerian hormone (AMH)--is it a new reliable marker of the ovarian reserve? Its role in predicting the ovarian response in assisted reproductive technology (ART)].

    Alshiek, Jonia Amer; Lessing, Joseph B; Amit, Ami; Azem, Foad


    Anti-Müllerian hormone (AMH) is predominantly known for its important role in the differentiation of the male and female sexual system during the early embryonic period. Recently, many animal and human researches have been studying the role of the AMH in the postnatal ovarian function. In the female, AMH is produced by the granulosa cells of early developing follicles. It plays a major role in the folliculogenesis and seems to be able to inhibit the initiation of the growth of primordial follicles and FSH-induced follicles. As AMH is expressed throughout the folliculogenesis, from the primary follicular stage to the antral stage, the serum levels of AMH may represent both the quantity and the quality of ovarian follicles. Thus, the AMH levels may be useful as a new potential marker of the ovarian reserve. As compared to other ovarian reserve tests, the AMH has unique characteristics which make it a favorable marker. The measurement of AMH levels may be useful in the prediction of poor response and cycle cancellation as well as hyper-response and the ovarian hyperstimulation syndrome in assisted reproductive technology (ART). We assume that the measurement of AMH Levels may play a role in the individualization of treatment strategies among patients who are treated by ART. However, the AMH cannot predict the qualitative ovarian response in ART. In men, the AMH was not found to have satisfactory clinical utility as a single marker of spermatogenesis. PMID:23002694

  8. Allelic variant in the anti-Mullerian hormone gene leads to autosomal and temperature-dependent sex reversal in a selected Nile tilapia line.

    Stephan Wessels

    Full Text Available Owing to the demand for sustainable sex-control protocols in aquaculture, research in tilapia sex determination is gaining momentum. The mutual influence of environmental and genetic factors hampers disentangling the complex sex determination mechanism in Nile tilapia (Oreochromis niloticus. Previous linkage analyses have demonstrated quantitative trait loci for the phenotypic sex on linkage groups 1, 3, and 23. Quantitative trait loci for temperature-dependent sex reversal similarly reside on linkage group 23. The anti-Müllerian hormone gene (amh, located in this genomic region, is important for sexual fate in higher vertebrates, and shows sexually dimorphic expression in Nile tilapia. Therefore this study aimed at detecting allelic variants and marker-sex associations in the amh gene. Sequencing identified six allelic variants. A significant effect on the phenotypic sex for SNP ss831884014 (p<0.0017 was found by stepwise logistic regression. The remaining variants were not significantly associated. Functional annotation of SNP ss831884014 revealed a non-synonymous amino acid substitution in the amh protein. Consequently, a fluorescence resonance energy transfer (FRET based genotyping assay was developed and validated with a representative sample of fish. A logistic linear model confirmed a highly significant effect of the treatment and genotype on the phenotypic sex, but not for the interaction term (treatment: p<0.0001; genotype: p<0.0025. An additive genetic model proved a linear allele substitution effect of 12% in individuals from controls and groups treated at high temperature, respectively. Moreover, the effect of the genotype on the male proportion was significantly higher in groups treated at high temperature, giving 31% more males on average of the three genotypes. In addition, the groups treated at high temperature showed a positive dominance deviation (+11.4% males. In summary, marker-assisted selection for amh variant ss831884014

  9. The correlation ofseminal anti -mullerian hormone and infertility semen parameters%精浆抗苗勒氏管激素与不育症精液参数的相关性研究

    张志杰; 陈小均; 赵萌; 张岳阳; 段平川; 贾玉森


    Objectives:To explore the correlation of seminal anti -mullerian hormone (AMH)levels and male infertility semen parameters.Methods:56 patients in the male infertility clinic were selected as infertility and another 41 healthy male with reproductive history were selected as the control group.The seminal plasma AMH and semen parameters (sperm concentration,motility,viability and malformation rate)in both groups were detected. The serum sex hormone of infertility group was detected.Results:1 .Infertility group and control group had no sig-nificant difference in age and abstinence time.2.AMH was positively correlated (P <0.01 )with class A sperm rate,sperm motility and sperm viability,and correlation with morphological.The infertility group and the control group had no significant difference in seminal AMH value.3.Seminal AMH of infertile patients with normal semen analysis was significantly higher than in patients with weak sperm (P≤0.01 ),and had no significant difference in normal and abnormal morphologically.In infertile group,Seminal AMH had no significant correlation with sex hor-mones.Conclusions:The seminal AMH level is positively correlated with class A sperm rate,sperm motility and sperm viability.With the improvement of semen analysis results,seminal AMH is improved,regardless of the state of infertility.Seminal AMH can be used as indicators in clinical testing of infertility treatment effect.%目的:探讨精浆中抗苗勒氏管激素(AMH)水平与男性不育症精液参数的相关性。方法:选取门诊就诊的男性不育症患者56例为不育组,另设有生育史的健康男性41例作为正常对照组,两组均检测精浆中抗苗勒氏管激素(AMH)、精液参数(精子的密度、活率、活力及畸形率),不育组测定血清性激素6项。结果:1.不育组与正常对照组年龄、禁欲时间无明显差异;2.不育组及正常对照组精浆 AMH 与 a 级精子率、精子活力、精

  10. The effect of serum inhibin B and anti-mullerian hormone measurement for predicting ovarian reserve and treatment options in assisted reproduction%抑制素B和抗苗勒管激素预测卵巢反应性

    吴日然; 叶云; 廖月婵; 杜静


    近年来应用抑制素B(inhibinB,INHB)、抗苗勒管激素(anti-mullerian hormone,AMH)水平等做为预测卵巢储备功能的指标。INHB是转化生长因子β超家族的成员,由卵巢中小窦卵泡颗粒细胞直接产生,主要生理作用是反馈性抑制垂体卵泡刺激素(FSH)分泌,其在年龄上的变化早于FSH水平,

  11. 盆腔炎性疾病患者血清AMH水平规律初探%Study on the level of anti-Mullerian hormone in patients with pelvic inflammatory disease

    杨旭辉; 张杰; 黄志承; 梁嘉颖; 杨少芬; 朱照平; 何峰; 刘风华


    目的 调查不同程度盆腔炎性疾病(pelvic inflammatory disease,PID)患者血清抗苗勒管激素(anti-Mullerian hormone,AMH)水平并与正常生育期女性血清AMH水平比较,以探讨PID患者AMH的表达规律及临床意义.方法 选择健康女性67例为对照组,轻中度PID患者121例、重度PID患者34例为观察组,各组再按年龄分为5组:<25岁A组、26 ~ 30岁B组,31 ~35岁C组、36 ~ 40岁D组、>40岁E组.ELISA法检测血清AMH水平并分析各组不同年龄段的分布规律及表达差异.结果 轻中度PID患者血清AMH平均(4.48±0.36) ng/mL,与年龄呈负相关(r=-0.432,P<0.01),即AMH随年龄的增长不断下降;A组、B组均值与C组、D组、E组比较差异均有统计学意义(P<0.01),E组与A、B、C组比较差异也有统计学意义(P<0.01).重度PID患者血清AMH平均(2.12±0.26) ng/mL,与年龄呈负相关(r=-0.428,P<0.05),AMH随着年龄的增长下降幅度不大,但40岁后女性AMH骤降;E组与其余各组比较,差异均有统计学意义(P<0.01).对照组血清AMH平均(3.91±0.51) ng/mL,与年龄呈负相关(r=-0.742,P<0.01),即AMH随年龄的增长不断下降;E组与其余各组两两比较,差异均有统计学意义(P<0.01),A、B组与C、D、E组两两比较差异也有统计学意义(P<0.05).不同年龄组重度PID患者与轻中度PID患者、对照组血清AMH均值比较差异有统计学意义(P<0.05).结论 年龄是影响PID患者和正常生育期女性血清AMH水平的重要因素,重度PID患者与轻中度PID患者、正常女性血清AMH水平在不同年龄段均有差异;轻中度PID患者血清AMH水平可参照正常生育期女性的水平.

  12. Clinical study of anti-Mullerian hormone in prediction of ovarian aging%抗苗勒管激素与年龄的关系及其预测卵巢衰老的临床研究

    高丽虹; 蔡文伟; 朱琴; 李永祥; 金倩燕; 张梅光; 蔡英如; 周芝婷; 吴晓杰


    目的:研究不同年龄阶段月经正常的生育期妇女抗苗勒管激素(AMH)水平的变化,了解AMH与年龄的关系及其在预测卵巢衰老中的价值。方法选择2012年12月至2014年7月在嘉兴市妇幼保健院行健康体检的月经正常的1423例生育期妇女,按年龄阶段分为:≤25岁、26~30岁、31~35岁、36~40岁、≥41岁,检测其血清AMH水平,并分析与年龄的关系。结果≤25岁、26~30岁、31~35岁、36~40岁、≥41岁妇女的血清AMH水平中位数分别为3.62、3.10、2.27、1.07、0.45μg/L;不同年龄阶段妇女血清AMH水平比较,差异有统计学意义(H=232.386,P<0.01)。血清AMH水平与年龄呈负相关(r=-0.374,P<0.01);血清AMH水平在25岁后即开始下降,36岁后下降明显。logAMH (即AMH的对数)的二次回归拟合最佳地反映了AMH与年龄的关系。结论检测生育期妇女血清AMH水平可早期预示卵巢功能的下降,可作为预警卵巢衰老的指标。%Objective To explore the serum anti-Mullerian hormone (AMH) level in women of childbearing age with normal menstrual cycles. Methods A total of 1 423 women with regular menstrual cycles were selected and divided into 5 groups according to their ages, i.e.≤25, 26-30, 31-35, 36-40,≥41 years. Their serum levels of AMH were measured, and the relationship between AMH and age was analyzed. Results The serum AMH levels of 5 groups according to ages (≤25, 26-30, 31-35, 36-40, ≥41 years) were 3.62, 3.10, 2.27, 1.07, 0.45μg/L, respectively. The comparison of serum AMH levels in different age groups had significant difference (P<0.01). Serum AMH level declined with increasing age,and dropped significantly after 36. The serum AMH level and age showed a negative correlation with significant difference (r=-0.374, P<0.01). Quadratic regression of logAMH proximally reflected the relationship between AMH and age. Conclusion AMH determination for women of

  13. 血清抗苗勒管激素诊断多囊卵巢综合征的临床价值%Clinical value of serum anti-mullerian hormone in diagnosis of polycystic ovarian syndrome

    杨小岗; 王芳; 武佳敏; 董永生; 赵志刚; 武保乡; 武春燕


    Objective To explore the characteristic relationship between the anti-Mullerian hormone (AMH)and the PCOS hormone and use the ROC curve method to evaluate the diagnosis value of AMH for PCOS.Methods Used the enzyme-linked immunosorbent assay(ELISA)to detect the serum level of AHM,follicle-stimulating hormone(FSH),lunteinizing hormone (LH),testosterone(T),the estradiol(E2) and the LH/FSH value in 155 patients with PCOS,and carried on comparing with 134 women with normal menstrual cycle(control group).At the same time ,drawh up the ROC curve SPSS and statistical analyzed these datum.Results(1)The serum hormone levels(AMH 10.45 ng/ml,T 0.83 ng/ml.LH10.21 mIU /ml)of PCOS group were obviously higher thanthose of control group(AHM 5.14 ng/ml,T 0.77ng/ml,LH 5.04 mIU/ml)(P<0.01).The E2 level of PCOS group(68.2 pg/ml)was obviously lower than that of control group (116.05pg/ml)(P=0.000).LH/FSH ratio of the two groups had no remarkable differences(P=0.877).(2)The area under the ROC curve for PCOS diagnosed by AMH was 0.828.The standard error of area was 0.023 which has the remarkable significance(P=0.000);while the best dividing value of PCOS diagnosed by AMH was 6.865 ng/ml,the sensitivity was 75.48%,specificity was 73.63% and diagnostic effciency was medium .Conclusions Detecting serum AMH level is a novel and important method of PCOS diagnosis and research,but it is not suitable for taking the LH/FSH ratio as diagnosis indicator of PCOS.%目的 探讨抗苗勒管激素(AMH)与多囊卵巢综合征(PCOS)激素特征的关系及使用ROC曲线法评价AMH对PCOS的诊断价值.方法 用酶联免疫吸附法(ELISA)测定155例PCOS患者血清AMH、卵泡刺激素(FSH)、黄体生成素(LH)、睾酮(T)、雌二醇(E2)水平及LH/FSH值,并与134例月经周期正常妇女进行对照.用SPSS绘制ROC曲线及统计分析.结果 (1)PCOS组血清激素(中位数)AMH 10.45 ng/ml、T0.83 ng/ml、LH 10.21 mIU/ml水平,明显高于对照组(AMH 5.14 ng/ml、T0.77ng/ml、LH 5.03 mIU

  14. 抗苗勒管激素评价子宫肌瘤剔除术后卵巢功能的改变%The effect on ovarian function after myomectomy assessed by serum anti-Mullerian hormone levels

    王海英; 梁洁玲; 张仁礼; 毕燕玲; 罗怀钦; 蒋洲梅; 闻安民


    Objective The aim of this prospective study was to evaluate the changes in the ovarian reverse after myomectomy based on serum anti-Mullerian Hormone (AMH) levels. Methods This is a prospective longitudinal observational study. Serum AMH levels were measured at the baseline and 2 day , 3months after myomectomy in 35 women aging from 36 to 45years.Follicle stimulate hormone(FSH) and luteal hormone(LH) were measured at the same time. 35 women of the same age with fibroid who did not undergo operation were selected as control group. Result (1)AMH level is (1.54 ± 0.95)ng/mL,(1.18 ± 0.77)ng/mL,(1.50 ± 0.58 )ng/mL at 0 day, 2 days and 3 months after operation. AMH level decreased significantly at 2 days after operation (P 0.05).(2) Significant differences in the serial change of AMH levels existed at each time point between myomectomy group and control group (P <0.05). No significant differences in FSH or LH levels existed at each time point. Conclusion AMH is may be superior to FSH or LH in evaluating the changing of ovarian reverse. The study suggests that myomectomy affect the ovarian function for up to 3 months post-operatively , and hemorrhage during and after operation may decrease serum AMH levels.%目的:通过检测新的卵巢功能指标抗苗勒管激素(AMH),评估子宫肌瘤剔除术对于年轻患者卵巢功能的影响。方法:前瞻性队列观察研究,选取36~45岁因子宫肌瘤行子宫肌瘤剔除术患者35例,于术前,术后2 d,术后3个月检测血清 AMH、促卵泡生成素(FSH)、促黄体生成激素(LH)水平,选取同年龄组未行手术的35例子宫肌瘤患者为对照组,比较两组间及各时间点各激素水平的变化差异。结果:(1)子宫肌瘤剔除组患者术前、术后2 d、术后3个月AMH水平分别为(1.54±0.95)ng/mL,(1.18±0.77)ng/mL,(1.50±0.58)ng/mL,术后2 d AMH 水平较术前显著降低(P<0.05),术后3个月AMH 水平逐

  15. Effect of testosterone on secretion of anti-Mullerian hormone in mouse ovarian granular cells cultured in vitro%睾酮对体外培养小鼠卵巢颗粒细胞分泌抗苗勒管激素的影响

    张颖; 郑金丹; 刘丽丽


    Objective To study the effect of testosterone on secretion of anti-Mullerian hormone (AMH) in mouse ovarian granular cells cultured in vitro.Methods Primary mouse ovarian granular cells were cultured in vitro and treated with testosterone at different concentrations. Expression of AMH in mouse ovarian granular cells was detected by ELISA.Results ELISA showed that the AMH was expressed in mouse ovarian granular cells. Twenty-four hours treatment of primary mouse ovarian granular cells with testosterone at the concentrations of 10-8 mmol/L, 10-7 mmol/L and 10-6 mmol/L up-regulated their secretion of AMH in a concentration- and time-dependent manner (P<0.01).Conclusion Testosterone up-regulates the secretion of AMH in mouse ovarian granular cells in a concentration-dependent manner.%目的:探讨睾酮对体外培养的小鼠卵巢颗粒细胞抗苗勒管激素(anti-Mullerian hormone,AMH)分泌的影响。方法小鼠卵巢颗粒细胞原代培养,添加不同浓度睾酮,ELISA法检测颗粒细胞AMH的表达。结果 ELISA法证实颗粒细胞中有AMH表达,不同浓度(10-8 mmol/L、10-7 mmol/L、10-6 mmol/L)睾酮作用24 h可促进颗粒细胞分泌AMH,且随睾酮浓度的增加、作用时间延长,AMH分泌明显增加(P<0.01)。结论睾酮促进小鼠卵巢颗粒细胞分泌AMH,高浓度比低浓度作用更强。

  16. 抗苗勒管激素在子宫全切术后卵巢功能评价中的研究%The role of anti-Mullerian hormone on the evaluation of ovarian function after total hysterectomy

    尹红燕; 刘玉珍; 吕秀萍; 张鹏; 孙小云


    Objective To investigate the value of anti-Mullerian hormone (AMH)in evaluating ovarian function after total hysterectomy and the influence of total hysterectomy on ovarian function. Methods This study was conducted at the affiliated hospital of Weifang Medical College between February and November 2013.Thirty patients aged 40 and 50 years who underwent laparoscopic total hysterectomy for benign uterine diseases were enrolled into the case group and unaffected women in a similar age range constituted the control group.Serum levels of AMH,follicle-stimulating hormone (FSH),luteinizing hormone (LH)and estradiol (E2 )were assayed on the 7th to 10th day of menstruation before operation,at 1st month and 6th month of operation,respectively.At the same time,ovarian volume (OV)and ovarian stromal artery resistance index (RI)were measured through transvaginal ultrasound. Results In the case group,there were significant differences between pre-operation and at 1st month of operation in AMH [(1.49 ±075)μg/L vs.(1.10 ± 0.48)μg/L],FSH [(9.45 ±4.05)U /L vs.(12.44 ±6.76)U /L],LH [(10.02 ±7.90)U /L vs.(13.97 ±7.28) U /L],E2 [(55.55 ±14.68)ng/L vs.(44.45 ±13.81)ng/L],OV [(6.63 ±2.37)cm vs.(3.57 ±1.03)cm,and FI [(0.59 ±0.20)vs.(0.73 ±0.08)].However,only AMH levels showed a statistical significance between pre-operation and at 6th month of operation [(0.72 ±0.59)μg/L].In the control group,Levels of AMH,FSH,LH and E2 levels,OV and RI at 1st and 6th month of operation were insignificantly difference compared with preoperative levels. There were significant differences at 1st month of operation between the case group and the control group in AMH [(1.47 ± 0.75)μg/L,FSH (9.56 ±3.61)U /L,LH (9.94 ±6.04)U /L,E2 (51.53 ±14.60)ng/L],OV [(6.71 ±2.36)cm, and RI (0.69 ±0.21)].Only AMH level was significant different between case group and control group [(1.46 ±0.83)μg/L]at 6th month of operation. Conclusion It appears that total hysterectomy causes more or less loss of

  17. Elderly men have low levels of anti-Mullerian hormone and inhibin B, but with high interpersonal variation: a cross-sectional study of the sertoli cell hormones in 615 community-dwelling men.

    Yih Harng Chong

    Full Text Available The Sertoli cells of the testes secrete anti-Müllerian hormone (Müllerian inhibiting Substance, AMH and inhibin B (InhB. AMH triggers the degeneration of the uterine precursor in male embryos, whereas InhB is part of the gonadal-pituitary axis for the regulation of sperm production in adults. However, both hormones are also putative regulators of homeostasis, and age-related changes in these hormones may therefore be important to the health status of elderly men. The levels of AMH in elderly men are unknown, with limited information being available about age-related changes in InhB. We have therefore used ELISAs to measure Sertoli cell hormone levels in 3 cohorts of community-dwelling men in New Zealand. In total, 615 men were examined, 493 of which were aged 65 or older. Serum AMH and InhB levels inversely correlated with age in men older than 50 years (p<0.001 but not in the younger men. A minority of elderly men had undetectable levels of AMH and InhB. The variation in hormone levels between similarly aged men increased with the age of men. AMH and InhB partially correlated with each other as expected (r = 0.48, p<0.001. However, the ratio of the two Sertoli hormones varied significantly between men, with this variation increasing with age. Elderly men selected for the absence of cardiovascular disease had AMH levels similar to those of young men whereas their InhB levels did not differ from aged-matched controls. These data suggests that Sertoli cell number and function changes with age, but with the extent and nature of the changes varying between men.

  18. Relationship between anti-mullerian hormone in follicular fluid and telomere length in cumulus cells with oocytes at different mature stages%抗苗勒管激素及卵丘细胞端粒长度与卵母细胞成熟度的相关研究

    王兴玲; 蔡鹏飞; 张文娟; 肖雅琳


    目的:探讨体外受精-胚胎移植(in vitro fertilization-embryo transfer,IVF-ET)中卵泡液内抗苗勒管激素(anti-mullerian hormone,AMH)水平及对应的卵丘细胞端粒长度与卵母细胞成熟度的关系.方法:收集52例行IVF-ET患者卵泡液及卵丘细胞,依据每位患者卵母细胞成熟度,将成熟卵泡液、未成熟卵泡液分别混合标记为成熟的卵泡液组(follicular fluids of mature oocyte,FFMⅡ)、未成熟的卵泡液组(follicular fluids of immature oocyte,FFMI).从卵母细胞周围机械剥离的部分卵丘颗粒细胞同样依据卵母细胞成熟度分组,标记为成熟卵母细胞的颗粒细胞组(cumulus cells of mature oocyte,CCMⅡ)、未成熟卵母细胞的颗粒细胞组(cumulus cells of immature oocyte stage,CCMI).应用酶联免疫法(enzyme-linked immunosorbent assay,ELISA)检测卵泡液中AMH,实时定量PCR测定卵丘细胞端粒长度.结果:未成熟卵泡液组中AMH水平高于成熟组[(6.02±1.34) vs.(5.66±1.42)],差异未达到统计学意义(P=0.13);未成熟组卵丘细胞端粒长度较成熟组的短[(3.50±1.08) vs.(4.40±1.24)],差异有统计学意义(=0.000).结论:未成熟的卵泡液组中AMH水平高于成熟组,卵泡液中AMH可能与卵母细胞成熟度有关,卵丘细胞端粒长度可以反映卵母细胞成熟度.

  19. The values of serum and follicular fuild anti-mullerian hormone levels of PCOS patients on predicting the outcomes of IVF/ICSI%血清及卵泡液抗苗勒管激素在多囊卵巢综合征患者体外受精促排卵治疗中的价值

    陈雅; 叶碧绿; 郑九嘉; 林金菊; 赵军招; 张李雅; 杨旭


    目的:探讨长方案及拮抗剂方案的多囊卵巢综合征(polycystic ovary syndrome,PCOS)患者血清及卵泡液中抗苗勒管激素(anti-Mullerian hormone,AMH)水平对体外受精(IVF)结局的预测价值.方法:本前瞻性研究将59例行IVF治疗的PCOS患者随机分为口服避孕药后长方案(OC+ LP)30例和拮抗剂方案组29例,分别测定IVF治疗前一月经周期第2~5天(bAMH)、启动日(Gn日AMH)、取卵日(OPU日AMH)及卵泡液(FF AMH)的AMH水平,比较两组临床、实验室指标及AMH值,并分析AMH在促排卵过程中的变化.结果:OC+ LP组和拮抗剂组获卵数、成熟率、受精率、种植率及临床妊娠率等差异均无统计学意义(P>0.05),两组同一时间点AMH值比较差异均无统计学意义(P>0.05),无论是OC+LP组还是拮抗剂组,同一组内不同时间点AMH值比较显示,OPU日血清AMH水平均显著低于bAMH、Gn日AMH及FF AMH,差异具有统计学意义(P<0.05),而后三者两两相比,差异均无统计学意义(P>0.05).两组合并后按妊娠结局分临床妊娠组22例和非妊娠组20例,其年龄、获卵数、优质胚胎率、bAMH、Gn日AMH、OPU日AMH及FF AMH值差异均无统计学意义(P>0.05).结论:PCOS患者无论行长方案或拮抗剂方案,均不影响AMH对其卵泡发育的调控;血清及成熟卵泡液中AMH水平均不能预测PCOS患者IVF临床结局.

  20. 多囊卵巢综合征患者抗苗勒氏管激素与干细胞因子的相关性%Correlation between anti-mullerian hormone and stem cell factor in patients with polycystic ovary syndrome

    王飞苗; 罗艳; 马会明; 吴昕; 胡蓉


    目的 研究多囊卵巢综合征(PCOS)患者抗苗勒氏管激素(AMH)和干细胞因子(SCF)的表达情况及二者的相关性.方法 选择年龄均小于35岁行体外受精与胚胎移植的PCOS患者102例,正常对照组90例,采用ELISA法检测血清和卵泡液中AMH和SCF的蛋白水平,分离并培养颗粒细胞;采用Real-time PCR检测PCOS患者颗粒细胞中AMH和SCF mRNA的相对含量,并对其进行相关性分析.结果 ①PCOS患者血清、卵泡液及颗粒细胞中AMH的相对表达较正常对照组明显升高,而SCF较对照组显著降低(P<0.01);②PCOS患者血清、卵泡液及颗粒细胞中AMH与SCF的表达均呈极为显著负相关(P<0.01).结论 多囊卵巢综合征患者血清、卵泡液及颗粒细胞中AMH与SCF的表达呈显著负相关.%To study the expressions of and correlation between anti-mullerian hormone (AMH ) and stem cell factor (SCF) in patients with polycystic ovary syndrome

  1. Serum Anti Mullerian Hormone (AMH) Tube Used in Infertility in Patients%血清抗缪勒氏管激素(AMH)在不孕不育症中的临床应用探讨

    单咏梅; 庄严; 杨凡; 万海英


    目的:通过检测不孕不育症患者血清中抗缪勒氏管激素(anti-mul erian hormone,AMH),及卵泡刺激素(Fol icle-stimulating hormone,FSH)、黄体生成素(luteinizing hormone,LH)、雌二醇(Estradiol,E2)的血清表达水平,并进行比对、分析,探索AMH在不孕不育症中的临床应用价值。方法用化学发光法在ADVIA Centaur XP仪器上检测LH、FSH、E2,再用酶联免疫法对AMH进行测定,最后将这些指标用EXCEL软件进行线性回归分析。结果①根据厂商提供的AMH参考值可知,在FSH、LH、E2特别高的39例不孕不育育龄女性的标本中,38例都在该年龄段的参考范围内,只有1例超过参考范围,但是在不同年龄范围内低于中值年龄的30例患者中,有90.9%的患者AMH浓度远低于参考范围给出的中值浓度;②根据对LH、FSH、E2和AMH线性回归分析可知,LH、FSH、E2和AMH没有直接的线性关系;③LH/FSH呈一定程度的正相关。结论根据不同年龄段的育龄妇女的AMH浓度在该年龄段是否偏低或者异常增高来在一定程度上评估其生育能力,并通过进一步研究更为细分不同年龄段的妇女AMH表达情况,并建立合适的相应参考区间。 LH、FSH、E2和AMH没有太大的线性关系,这可能与LH、FSH、E2在月经周期期间波动很大、搜集的标本为不同年龄的、而AMH浓度不受月经周期影响、会受年龄影响有关。此外,有相关文章表明,经过年龄校正后LH/FSH的比值与AMH呈部分相关。而本试验做出LH/FSH呈一定程度的正相关,LH/FSH的比值是一个有用可靠评估卵巢储备功能的指标,并且可以和AMH共同应用于临床评估。因此AMH可以在一定程度应用于不孕不育症。%Objective Through the detection of anti-Mul erian hormone and fol icle stimulating hormone, luteinizing hormone, estradiol, comparison analysis, to explore the application value of AMH in the infertility.Methods The detection of LH, FSH, E2 in

  2. The Expression of Anti-Mullerian Hormone and Its Type Ⅱ Receptor Gene Polymorphisms in Patients with Polycystic Ovarian Syndrome%抗苗勒管激素及其Ⅱ型受体基因多态性在多囊卵巢综合征患者表达的研究

    胡蓉; 姜银芝; 田春花; 田进石; 兰生学; 王飞苗; 贾韶彤; 张晓梅; 李彩艳; 杨海燕


    Objective:To investigate the gene polymorphism of the anti-mullerian hormone (AMH) and its type II receptor (AMHR II) in polycystic ovarian syndrome (PCOS), and to analyze the relationship of body mass index (BMI) and sex hormone levels in patients with different AMH gene types, so as to explore the possible mechanism of PCOS in gene level. Methods: Genotype distribution and allele frequencies of AMH and AMHR II were examined by polymerase chain reaction (PCR) and DNA forward and reverse sequencing in 94 PCOS patients and 94 normal women as control. The age, BMI and hormone levels of different AMH, AMHR II genotypes were compared. Results:①There was a significant difference of AMH genotype distribution in the two groups (P0.05); ②There were obvious difference of BMI, LH, T levels in PCOS patients with different AMH genotypes (P0.05). In the control group, there was no correlation between AMH genotype distribution and patient age, BMI and sex hormone levels( P >0.05). ③There were no correlations between the AMHR2 genotype distribution and patient age, BMI and sex hormone levels in either normal control or PCOS patients (P>0.05). Conclusions:AMH gene polymorphism may be associated with the pathogenesis of PCOS, also the G/T phenotype in AMH genotype may be the reason for obesity and hyperandrogenism in PCOS.%目的:研究抗苗勒管激素(AMH)及抗苗勒管激素Ⅱ型受体(AMHRⅡ)基因多态性在多囊卵巢综合征( PCOS)患者的表达情况;同时分析PCOS不同AMH基因型患者体重指数(BMI)、性激素水平之间是否存在差异,从基因水平探讨PCOS的发病机制.方法:采用聚合酶链反应(PCR)及DNA正、反向测序方法,检测94例PCOS患者(PCOS组)及94例正常排卵患者(对照组)AMH和AMHRⅡ基因型,同时将两组AMH、AMHRⅡ不同基因型患者的年龄、BMI及性激素水平分别进行比较.结果:①两组AMH基因型分布比较,差异有统计学意义(P<0.05),而AMH等位基因频率、AMHRⅡ基因

  3. 抗苗勒氏管激素在多囊卵巢综合征中的诊断价值初探%The Diagnostic Value of Serum Anti-Mullerian Hormone (AMH) Measurement in PCOS

    李琳; 翁梅英; 陈晓莉; 陈亚肖; 钟俊敏; 杨冬梓


    Objective:To detect the serum anti-Müllerian hormone (AMH) level of patients with polycystic ovary syndrome (PCOS), and to explore the diagnostic value of serum AMH measurement in PCOS.Methods:47 patients with PCOS and 40 healthy females were enrolled from June 2006 to December 2006 in the Second Affiliated Hospital of Sun Yat-sen University. Serum AMH levels were measured by enzyme linked immunosorbent assay (ELISA). Results: The mean serum AMH level in PCOS group was significantly higher than control group (9.85±4.93 ng/ml vs 7.13±3.02ng/ml, P=0.002), which is similar in adult PCOS patients and controls (10.64±5.47 ng/ml vs 7.01 ± 2.43 ng/ml, P=0.005). No difference was found in serum AMH of adolescent PCOS patients and controls (8.95± 4.18 ng/ml vs 7.26± 3.58 ng/ml, P = 0.168). The areas under the ROC curve for AMH in all PCOS population, adults and adolescents were 0.664 (P=0.008), 0.714 (P = 0.015), and 0.614 (P = 0.208), respectively. The best compromise between specificity (70.0%) and sensitivity (61.7%) was obtained with a cut-off value of 8 ng/ml. Conclusions: Serum AMH level was elevated in patients with PCOS. Serum AMH measurement offers a relatively poor sensitivity of 61.7% and a specificity of 70% with a cut off value at 8 ng/ml, suggesting the diagnostic value of AMH was limited.%目的:研究多囊卵巢综合征(PCOS)患者的血清抗苗勒氏管激素(AMH)水平,并初步探讨其在PCOS中的诊断价值.方法:收集2006年6月至12月在我院妇产科就诊的47例PCOS患者(研究组)和年龄匹配的40例健康女性(对照组),应用酶联免疫吸附(ELISA)法检测血清AMH水平.结果:研究组的血清AMH水平明显高于对照组(9.85±4.93 ng/ml vs 7.13±3.02 ng/ml,P=0.002).血清AMH的曲线下面积在总体人群为0.664(P=0.008),以血清AMH 8 ng/ml为相对最佳的诊断界值,敏感度为61.7%,特异度为70.0%.结论:PCOS患者的血清AMH水平明显增高,血清AMH测定对于PCOS的诊断效能较低.

  4. Correlation between anti-Mullerian hormone and follicle-stimulating hormone receptor mRNA expression in granulosa cells in vitro.%体外颗粒细胞培养中抗苗勒管激素与卵泡刺激素受体mRNA表达的相关性

    张迪; 桑丽英; 崔蓉; 苗竹林; 钟兴明; 韦相才


    目的 体外培养小鼠原代颗粒细胞培养液中,加入不同浓度的抗苗勒管激素(AMH),比较各组间小鼠颗粒细胞上卵泡刺激素受体(FSHR)mRNA的表达情况,探讨体外培养颗粒细胞中AMH与FSHRmRNA表达的关系.方法 选取4~5周的昆明雌性小鼠,腹腔注射孕马血清促性腺激素,48~54h后颈椎脱臼法处死小鼠,取双侧卵巢,采用机械分离法释放颗粒细胞,胰蛋白酶消化细胞,低速离心分离细胞,用含15%胎牛血清的DMEM/F12培养基置于37℃、5%CO2培养箱中培养.显微镜观察细胞形态,流式细胞术分离细胞,并用免疫荧光法对所培养的颗粒细胞进行鉴定.同时在体外培养的小鼠原代颗粒细胞培养液中加入不同浓度(5、10、20、30、40ng/mL)的AMH,测定每组培养基中AMH的终浓度,并用荧光定量PCR技术检测每组颗粒细胞中FSHR mRNA 的表达.结果 分离培养的颗粒细胞纯度大于80%;随AMH浓度加大,小鼠颗粒细胞中FSHR mRNA的表达逐渐降低,当AMH浓度为 5 和10ng/mL时,与对照组比较差异有统计学意义(P<0.05),且5 ng/mL浓度组颗粒细胞上FSHR mRNA的表达量最高.结论 用机械分离加胰酶消化加低速离心能得到纯度较高活性很好的颗粒细胞,用FSHR鉴定颗粒细胞是一种简便易行的方法,AMH浓度与颗粒细胞上FSHRmRNA表达呈一定负相关,表明AMH有可能通过抑制FSHR表达降低FSH敏感性,从而参与卵巢早衰的病理生理过程.%Objective To investigate the correlation between anti - Mullerian hormone (AMH) and follicle -stimulating hormone receptor (FSHR) mRNA expression in the cultured granulosa cells in vitro. Methods Kunming female mice were peritoneally injected with pregnant mare serum gonadotropin (PMSG) and sacrificed 48 to 54 hours after injection for collection of ovarian. Granulosa cells were isolated by mechanical method, trypsin digestion and low - speed centrifugation. Granulosa cells were diluted and incubated in

  5. Effect of follicle stimulating hormone on the secretion of anti-mullerian hormone in the granulosa cells in patients with polycystic ovarian syndrome%卵泡刺激素对多囊卵巢综合征患者卵巢颗粒细胞分泌抗苗勒管激素的影响

    李轶; 梁晓燕


    目的 研究卵泡刺激素(FSH)对多囊卵巢综合征(PCOS)患者卵巢颗粒细胞分泌抗苗勒管激素(AMH)的影响.方法 选择2008年8月至2009年12月于中山大学附属第六医院生殖中心就诊的33例PCOS患者,从其直径为8~10 mm的窦卵泡中分离颗粒细胞,并分为以下3组进行培养:未经FSH刺激的颗粒细胞(未刺激组,n=12);外源性FSH刺激的颗粒细胞(体外刺激组,n=12),此组颗粒细胞来源同未刺激组;对患者行FSH促排卵治疗,采集FSH体内刺激的颗粒细胞(体内刺激组,n=21).分别采用ELISA法和实时荧光定量PCR技术检测培养液中颗粒细胞分泌的AMH水平,及AHM mRNA表达水平;构建AMH荧光报告载体,检测卵巢颗粒细胞中AMH启动子的活性.结果 培养液中颗粒细胞分泌的AMH水平,未刺激组为(11.4±4.0)μg/L,体外刺激组为(7.9±1.1)μg/L,体内刺激组为(5.6±1.7)μg/L,体内、体外刺激组分别与未刺激组比较,差异均有统计学意义(P均<0.05).AMH mRNA表达水平未刺激组为2.5±1.2,体外刺激组为1.5±0.5,体内刺激组为1.1±0.7,未刺激组高于体内、外刺激组,分别比较,差异均有统计学意义(P均<0.05).未刺激组颗粒细胞中AMH启动子的活性为11.5±2.3,体外刺激组为8.7±2.4,体内刺激组为6.8±2.4,未刺激组高于体内、外刺激组,分别比较,差异均有统计学意义(P均<0.05).结论 FSH可能通过抑制PCOS患者卵巢颗粒细胞中AMH的启动子活性及其mRNA表达,抑制AHM的过度分泌,促进卵泡的生长发育.%Objective To study the effect of follicle stimulating hormone(FSH)on the secretion of anti-mullerian hormone(AMH)in granulosa cells in patients with polycystic ovarian syndrome(PCOS).Methods From Aug.2008 to Dec.2009,33 patients with PCOS in Reproductive Medicine Research Center,Sixth Affiliated Hospital of Sun Yat-sen University were collected from 8-10 mm antral follicles and classified into the following three groups: FSH

  6. 抗苗勒氏管激素检测对子宫切除术后患者卵巢功能的评价%Evaluation on ovarian function after hysterectomy by detection of serum anti-Mullerian hormone levels

    王海英; 梁洁玲; 毕燕玲; 张仁礼; 罗怀钦; 蒋洲梅; 闻安民


    目的:探讨检测新的卵巢功能评价指标抗苗勒氏管激素(AMH)的价值,阐明子宫切除术对于年轻患者卵巢功能的影响。方法:选取36~45岁因子宫良性病变行全子宫切除术(子宫切除组)和同年龄段子宫肌瘤未行手术治疗(对照组)患者各35例,于不同时间点检测患者血清 AMH、促卵泡生成素(FSH)和促黄体生成激素(LH)水平,同时行阴道彩色多普勒超声监测卵巢动脉血流阻力指数(RI),分析不同时间点2组患者激素水平及卵巢动脉血流 RI的变化。结果:与术前比较,子宫切除组患者术后2 d及3个月 AMH 水平均明显降低(P<0.05或P<0.01)。与对照组比较,子宫切除组患者术后2 d及3个月血清 AMH 水平也明显降低(P<0.05)。子宫切除组患者术后1和3个月卵巢动脉血流 RI较术前和对照组明显增高(P<0.05)。子宫切除组和对照组患者不同时间点血清 FSH和 LH水平无明显变化(P>0.05)。结论:子宫切除术影响年轻患者卵巢功能,术后3个月 AMH水平下降,卵巢血流阻力增高。以血清 AMH 水平评估卵巢功能的改变优于传统的FSH和 LH指标。%Objective To evaluate the value of the new ovarian function marker serum anti-Mullerian hormone (AMH)and to clarify the effect of hysterectomy on the ovarian function of the younger women.Methods The serum AMH,follicle stimulating hormone (FSH)and luteinizing hormone (LH)levels in 35 women suffered uterus benign lesion aged 36-45 years and 35 women suffered hysteromyoma without operation were measured at different time.And the ovarian arterial blood flow resistance index (RI ) was measured by Doppler ultrasound. Results Compred with before operation, the serum AMH levels of the patients in hysterectomy group 2 d and 3 months after operation were significantly decreased (P0.05).Conclusion Hysterectomy can affect the ovarian function,and the serum AMH level

  7. Anti-Mullerian Hormone: Molecular Mechanism of Action

    J.A. Visser (Jenny)


    textabstractDuring early fetal development. the primitive urogenital system is bipotential and can develop into a male or female direction, depending on the chromosomal sex of the fetus. In this chapter, regulatory factors of sex determination and differentiation are described. The roles of two test

  8. Association between Gene Polymorphism of Anti-Mullerian Hormone and Its Type Ⅱ Receptor and Ovarian Reserve%抗苗勒管激素及其Ⅱ型受体基因多态性与卵巢储备功能的关系

    田春花; 胡蓉; 贾韶彤; 兰生学; 田进石; 王飞苗; 李彩艳; 杨海燕; 姜银芝


    Objective To investigate gene polymorphism of the anti-Mullerian hormone (AMH) and its type JJ receptor (AMHRTJ)in polycystic ovary syndrome (PCOS) and premature ovarian fail ure ( POF). Methods Polymerase chain reaction (PCR)and DNA sequencing were used to detect the distribution of AMH and AMHR JJ genotype and allele frequency in 94 cases of PCOS,94 normal women and 32 cases of POF. The correlation between AMH and AMHR JJ genotypes with age, body mass index(BMI)and hormone levels was analyzed. Results ? There was significant difference in AMH and AMHR JJ genotypes ,and AMH allele frequency among three groups of subjects(P 0. 05) ;? In PCOS patients, AMH genotype was correlated with BMI,LH,T levels(P0. 05);? In POF patients, AMHR H genotypes were associated with BMI,T and E2(P0. 05). Conclusion AMH and AMHRJJ gene polymorphism may be associated with PCOS,POF.%目的 探讨抗苗勒管激素(AMH)及其Ⅱ型受体(AMHRⅡ)基因多态性与卵巢储备功能的相关性.方法 采用聚合酶链反应(PCR)及DNA测序方法,检测94例多囊卵巢综合征(PCOS)患者、94例正常排卵女性及32例卵巢功能早衰(POF)患者AMH和AMHRⅡ基因型及等位基因频率的分布情况,分析患者AMH、AMHRⅡ基因型不同与其年龄、体重指数(BMI)及激素水平的相关性.结果 ① 3组研究对象AMH、AMHRⅡ基因型及AMH等位基因频率分布存在差异(均P<0.05),而AMHRⅡ等位基因频率分布无差异(均P>0.05);②PCOS患者AMH基因型与BMI、LH、T水平相关(均P<0.05),而正常对照组和POF患者AMH基因型与年龄、BMI、FSH、LH、T、E2、PRL水平无关(均P>0.05);③POF患者AMHR基因型与BMI、T及E2水平相关(均P<0.05),而正常组和PCOS患者AMHRⅡ基因型分布与其年龄、BMI、FSH、LH、T、E2、PRL水平均无关(均P>0.05).结论 AMH及AMHRⅡ基因的多态性可能与卵巢储备功能异常有关.

  9. 不孕不育患者抗子宫内膜抗体、抗精子抗体与抗苗勒管激素的检测价值%Detective value of anti endometrial antibody,antisperm antibody and anti-mullerian hormone in patients with infertility



    目的:探讨不孕不育患者抗子宫内膜抗体(EMAb)、抗精子抗体(AsAb)与抗苗勒管激素(AMH)的检测价值。方法选取2011年5月~2014年5月我院就诊的不孕不育夫妇80对,另选择同期健康及近3年内有生育史夫妇60对作为对照组。采用酶联免疫吸附试验测定比较两组患者EMAb、AsAb和AMH的情况。结果不孕不育组血清EMAb、AsAb阳性率和AMH水平均明显高于对照组,差异有统计学意义(P<0.05);不孕不育患者联合检测EMAb、AsAb阳性率明显高于单独检测EMAb、AsAb,差异有统计学意义(P<0.05)。不孕不育组精液异常患者AsAb阳性率明显高于精液正常者,差异有统计学意义(P<0.05)。结论 AsAb、EMAb和AMH与不孕不育的发生密切相关,联合检测EMAb、AsAb在不孕不育的诊断及治疗监测方面具有重要的价值。%Objective To investigate the detective value of anti endometrial antibody(EMAb),antisperm antibody(AsAb)and anti-mullerian hormone(AMH)in patients with infertility.Methods80 couples of infertility who were treated in our hospital from May 2011 to May 2014 were selected,and 60 healthy couples with fertility history in the last 3 years were selected as the control group.EMAb,AsAb and AMH in the two groups were determined by enzyme-linked immunosorbent assay and compared. Results The positive rates of serum EMAb,AsAb and level of serum AMH in infertility group were significantly higher than those in the control group,the difference was statistically significant(P<0.05);and the positive rate of combined detection of EMAb and AsAb in patients with infertility was significantly higher than that of single EMAb or AsAb,the difference was statistically significant(P<0.05).The positive rate of AsAb in infertile group with abnormal sperm was significantly higher than that with normal sperm, and the difference was statistically significant(P<0.05).ConclusionAsAb,EMAb and AMH are closely

  10. Predictive Value of Anti-mullerian Hormone on Ovary Response in PCOS Patients with Clomiphene Citrate (CC)-resistant after Laparoscopic Ovarian Drilling%抗苗勒管激素对氯米芬抵抗的PCOS妇女腹腔镜术后卵巢反应性预测价值

    韦成厚; 沈宏伟; 朱国平


    [Objective] To investigate the predictive diagnostic value of preoperation serum anti-mullerian hormone (AMH) level measurement on ovary ovulation response after laparoscopic ovarian drilling (LOD) in PCOS patients with clomiphene citrate (CC)-resistant. [Methods] 54 PCOS patients with clomiphene citrate(CC)-resistant were enrolled LOD. Serum AMH levels were measured by enzyme linked immunosorbent assay (ELISA) , Follicle growth and ovulation situation were supervised by vaginial ultrasound and urine LH peak. Using ROC (receiver operating characteristic) curve analysis monitoring data. [Results] ROC curve analysis indicated that AMH had moderate diagnostic value for postoperation high and poor ovary response. Theirs AUC (area under the curve, AUC) were 0.792 and 0.846 (95%CI: 0.673-0.911 and 0.696-0.996; P = 0.000 and P = 0.002). Serum AMH at 7.4 ng/mL and 4.3 ng/ mL were optium cutoff value for predictive high and poor ovary response, respectively. Sensitivity were 73.1% and 87.5% , respectively. Specificity were 71.4% and 66.7%, respectively. Positive predictive value were 71.9% and 72.4%, respectively. Negative predictive values were 72.6% and 84.2%, respectively. [Conclusions] The preoperation measurement of serum basal AMH level can predictive the high and poor ovary response after LOD in PCOS patients with clomiphene citrate (CC)-resistant, and act as a single valued predictive index in estimating LOD effectiveness.%[目的]探讨测定术前血清抗苗勒管激素(AMH)水平对氯米芬(CC)抵抗的多囊卵巢综合征(PCOS)妇女腹腔镜术后卵巢反应性预测价值.[方法]应用酶联免疫吸附(ELISA)法检测腹腔镜卵巢打孔术(LOD)54例氯米芬抵抗的PCOS患者术前血清基础AMH水平,术后用B超和尿LH峰监测卵巢卵泡发育及排卵情况,并用ROC曲线分析监测结果.[结果]ROC曲线分析表明,AMH对术后卵巢高和低反应性均为中等诊断价值,其AUC分别是0.792和0.846(95%CI:0.673-0.911和0.696-0.996;P

  11. 腹腔镜下次全子宫与全子宫切除、肌瘤剔除术对子宫肌瘤患者卵巢储备功能的影响%Effects of three surgical modalities on ovarian reserve by assessing the serum level of anti-Mullerian hormone for uterine fibroids

    袁华; 赵绍杰; 龚华; 梁喜; 王沂峰


    To compare the effects of laparoscopic total or subtotal hysterectomy and myomectomy on ovarian reserve by measuring the serum level of anti-Mullerian hormone (AMH).A total of 96 patients with uterine fibroids underwent total laparoscopic hysterectomy (TLH group,n =32),laparoscopic subtotal hysterectomy (LSH group,n =31) and laparoscopic myomectomy (LM group,n =33).Changes in ovarian reserve were examined by measuring the level of AMH pre-operation and at 1 and 4 months post-operation.No significant differences existed in AMH in LM group[(1.42 ±0.65),(1.31 ±0.53) & (1.33 ±0.61) μg/L,P > 0.05].The levels of AMH in both groups were significantly lower than those at pre-operation [(1.17 ± 0.11),(1.01 ±0.10),(0.48±0.54) & (1.18±0.93),(0.45 ±0.39),(0.14±0.0) μg/L,P<0.001].The mean percentage decrease in AMH was lower in TLH group than that in LSH group at 4-month follow-up [(-37.4 ± 37.6) % vs.(-27.0 ± 23.0) %,P =0.017].The results showed that LM had no effect on ovarian reserve.Both LSH and TLH had adverse effects on ovarian reserve and TLH was more marked.%对行腹腔镜子宫肌瘤剔除术的33例(剔除组)、腹腔镜次全子宫切除术的31例(次全切组)和腹腔镜全子宫切除术的32例(全切组),于术前、术后1和4个月行血清抗苗勒管激素(AMH)水平测定.剔除组手术前后血清AMH水平比较差异无统计学意义[(1.42 ±0.65)、(1.31±0.53)和(1.33±0.61) μg/L,P>0.05];次全切组和全切组术后血清AMH水平均较术前显著下降[(1.17±0.11)、(1.01±0.10)、(0.48 ±0.54)和(1.18 ±0.93)、(0.45-0.39)、(0.14 ±0.00) μg/L,均P<0.001];全切组术后4个月血清AMH水平下降百分率高于次全切组[(-37.4±37.6)%比(-27.0±23.0)%,P=0.017].提示肌瘤剔除术对卵巢储备无显著影响,全子宫切除术的影响最大.

  12. 腹腔镜下卵巢良性囊肿剥除术对患者血清抗苗勒管激素水平的影响%Effect of Laparoscopic Cystectomy on Serum Anti-Mullerian Hormone Level in Patients with Diverse Benign Ovarian Cysts

    奚晓雪; 杨晨; 戴建荣


    Objective To evaluate the effect of laparoscopic cystectomy on ovarian reserve in patients with diverse benign ovarian cysts by investigating the serum anti-Mullerian hormone (AMH) level before and after operation.Methods A total of 46 patients who underwent laparoscopic cystectomy due to unilateral ovarian cyst from April 2014 to April 2015 were recruited in this retrospective analysis. All patients were divided into endometrioma group and nonendometrioma group by pathological types. The surgery hemostatic method was bipolar electrocoagulation. 25 females in the same age were selected as the normal control group (NCG). Serum AMH levels were measured before operation and at 3 days, and 6 months follow-up visit afterwards.Results There were no signiifcant differences between nonendometrioma group and NCG in terms of serum AMH levels before operation. Serum AMH level was found to be lower in endometrioma group than that in nonendometrioma group and NCG before operation. Serum AMH level was found to be lower in both nonendometrioma group and endometrioma group after operation, AMH more markedly declined in endometrioma group at postoperative 3 days. At 6 months postoperatively, AMH levels were found to be similar between two groups.Conclusion Laparoscopic cystectomy could impair the ovarian reserve to some extent in patients with ovarian cysts in the immediate future and the effects could be related with the pathological patterns. The influence seems to be more obvious in patients with endometrioma.%目的:通过检测手术前后卵巢良性囊肿患者血清抗苗勒管激素(AMH)水平的变化,探讨腹腔镜下囊肿剔除术对卵巢储备功能的影响。方法选取2014年4月—2015年4月因单侧良性卵巢囊肿行腹腔镜下卵巢囊肿剔除术的46例患者,根据术后病理将患者分为巧囊组(n=23)、非巧囊组(n=23),术中创面止血采用双极电凝法。同时选取25例正常育龄妇女为对照组。于术前2 d

  13. 促性腺激素释放激素激动剂降调节对血清抗苗勒管激素的影响%Influence of Gonadotropin-releasing Hormone Agonist on Serum Anti-Mullerian Hormone Level

    叶云; 吴日然; 林晓丽; 廖月婵; 杜彦; 吴嘉齐; 林秀峰; 杜静


    [Objective] To investigate whether the influence of two forms (long-acting / short-acting) of gonadotrophin-releasing hormone agonist (GnRH-a) on anti-Mullerian hormone (AMH) is the same.[Methods] The study included 869 cycles that underwent in vitro fertilization (IVF) /intracytoplasmic sperm injection (ICSI) from February 2012 to September 2012.Patients who met the inclusion criteria were randomly divided into two groups:long-acting group (using long-acting GnRH-a,Diphereline(R)) which including 128 women with 128 cycles,and short-acting group (useing short-acting GnRH-a,Diphereline(R)) which including 116 women with 116 cycles.Serum AMH levels were measured at baseline and Day 14 following GnRH-a.[Results] ① After down-regulation in long-acting group,baseline serum AMH slightly reduced,P > 0.05; In short-acting group,serum AMH fell from (2.92± 2.12) μg/L to (2.29 ± 1.54) μg/L (P < 0.05).② After GnRH-a treatment higher basal AMH level fell down,and lower basal AMH level grew up in both groups.Using rising or falling AMH level as the standard to the ROC curve,we found the cutoff point of serum AMH:2.15 μg/L in long-acting group,and 2.16 μg/L in short-acting group.③In long-acting group,based on the cutoff points,basal AMH level of 52 women was less than 2.15 μg/L,and their basal serum AMH was (1.39 ± 0.54) μg/L,after down-regulation AMH was (2.73 ±-1.83) μg/L(P <0.01); 76 women with basal serum AMH ≥2.15 μg/L,their basal serum AMH was (4.40 ± 1.95) μg/L,after down-regulation was (3.27 ± 1.78) μg/L(P < 0.01).In short-acting group,51 women with basal serum AMH < 2.16 μg/L,AMH before down-regulation was (1.21 ± 0.50) μg/L,after down-regulation was (1.96 ± 1.57) μg/L (P <0.01) ; 65 women with basal serum AMH ≥2.16 μg/L,their basal serum AMH was (4.26 ± 1.93) μg/L,after down-regulation was (2.55 ± 1.48) μg/L (P < 0.01).④In long-acting group,for those whose AMH level < 2.15 μ g/L,there was no significant difference

  14. Influence of Tiaojing Zhuyun Fang Combined with Letrozole on the Situation of Follicular Development and Anti-Mullerian Hormone Level in Infertility Patients with Polycystic Ovary Syndrome%调经助孕方联合来曲唑对多囊卵巢综合征不孕患者卵泡发育及抗苗勒氏管激素的影响

    党慧敏; 刘艳巧; 刘润侠; 陈薇


    目的 研究中药调经助孕方联合来曲唑对多囊卵巢综合征(PCOS)不孕患者卵泡发育及抗苗勒氏管激素(AMH)的影响,探讨其作用机制.方法 将60例PCOS不孕患者随机分为3组,每组20例.中药组服用调经助孕方,西药组服用来曲唑,中西药组采用调经助孕方联合来曲唑治疗.比较3组患者治疗2个疗程后月经改善情况、基础体温(BBT)变化、排卵恢复情况、血清性激素及AMH水平.结果 治疗后中药组排卵率为45%(9例),西药组为60%(12例),中西药组为85%(17例),中西药组高于中药组及西药组(P<0.05);中药组受孕率为40%(8例),西药组为45%(9例),中西药组为80%(16例),中西药组高于中药组及西药组(P<0.05).治疗后较治疗前各组血清性激素雌二醇、孕酮水平均升高,黄体生成素、睾酮、AMH水平均降低(P<0.05).结论 调经助孕方联合来曲唑对PCOS不孕患者有较好的促排卵作用,并可提高受孕率,其作用机制可能与调节AMH水平有关.%Objective To investigate the influences of Tiaojing Zhuyun Fang (TJZYF) combined with letrozole on the situation of follicular development and anti-Mullerian hormone (AMH) level in infertility patients with polycystic ovary syndrome (PCOS), and explore its mechanism. Methods Sixty infertility patients with PCOS were divided into three groups randomly with 20 cases in each group. The Chinese medicine group was treated by TJZYF, the western medicine group was treated by letrozole, and the integrative medicine group was given both of them. The menstruation improvement, the change of the basal body temperature (BBT), spontaneous ovulation recovery, serum sexual hormones and AMH of the three groups after 2 courses of treatment were compared. Results After the treatment, the ovulation rate in the Chinese medicine group was 45% (9 cases), western medicine group was 60% (12 cases), while the integrative medicine group was 85% (17 cases). The ovulation

  15. Anti-Mullerian Hormone (AMH)Levels and its Relationships with Insulin Resistance and Androgen in Women with Polycystic Ovary Syndrome%PCOS患者血清抗苗勒管激素(AMH)水平与胰岛素抵抗及雄激素的关系

    肖琳; 李启富; 唐良萏


    研究多囊卵巢综合征(PCOS)患者血清抗苗勒管激素(AMH)水平的变化及其与胰岛素抵抗(IR)和雄激素的关系.方法:回顾性分析85例PCOS患者及79例正常女性的体质量指数(BMI)、血清AMH、血糖、胰岛素、雄激素、稳态模型胰岛素抵抗指数(HOMA-IR)间的相互关系.血清AMH采用ELISA法测定,空腹血浆葡萄糖(FPG)采用葡萄糖氧化酶法测定,空腹胰岛素(FIns)采用化学发光法测定,睾酮(T)采用酶联免疫法测定.结果:PCOS组血清AMH水平明显高于对照组;Person相关分析表明,PCOS组血清AMH水平与HOMA-IR及BMI不相关,与雄激素、年龄相关.多元线性回归分析显示,PCOS组中血清雄激素水平是血清AMH的影响因素.结论:PCOS人群中血清AMH较正常女性升高,但其升高与IR无关,而与雄激素水平密切相关.%Objective: To explore the relationships among insulin resistance (IR), androgens (T) and anti-Miillerian hormone (AMH) in women with polycystic ovary syndrome (PCOS). Methods: Eighty-five PCOS patients and 79 non-PCOS candidates were retrospectively analyzed. Body hight and weight were measured to calculate body mass index (BMI). BMI were calculated. AMH levels, fast blood glucose, fast blood insulin and androgen were assessed. Homeostatic model assessment of tissue insulin sensitivity (HOMA-IR) was calculated. The relationships among AMH, insulin resistance, androgen and BMI in patients with and without PCOS were studied. Results: The serum level of AMH of PCOS patients was significantly higher than that of non-PCOS patients. Age and androgen level, but not BMI and HOMA-IR, showed significant correlation with AMH level in patients with PCOS. Multiple stepwise regression analysis demonstrated that serum androgen level was independently related to serum AMH level in patients with PCOS. Conclusion: The AMH level was elevated and androgen level may relate to AMH level in patients with PCOS.

  16. Study on Reference Range of Anti-Mullerian Hormone Level in the Serum for Healthy Women of Childbearing Age in Handan Area%邯郸地区健康育龄妇女血清抗苗勒氏管激素参考值范围研究

    杨小岗; 王芳; 武佳敏; 武春燕; 武保乡


    Objective To established the reference range of AMH in the serum by determinating the antt-Mullerian hormone level (AMH) in the serum and the relativity of reproductive age for healthy women of childbearing age in Handan area. Methods Used the Enzyme-linked Immunosorbent Assay (ELISA) to determine the AMH level of serum in the early follicle period (2~5 days menstrual cycle) or corpora luteum metaphase (4~8 days after BBT rised) .meanwhile,need to determine the six reproductive Hormone level of LH,FSH,T,E2,FRL and P for 165 cases with 20~50 years old healthy women of childbearing age. Results The AMH level of serum was distributed in the shape of abnormal status. So the reference range indicated in median (M) and scope. The AMH level of serum in the early follicle period and corpora luteum metaphase bdicated respectively 3. 87(0. 41~10. 69)ng/ml and 4. 64(0. 45—11. 64)ng/ml, which were not with the remarkable differences. The AMH level of serum in early stage and late stage indicated respectively 5.14(0.45~11.64)ng/ml and 2.02(0.41 —9. 03)ng/ml,which would with the remarkable differences (P< 0.001). The reference range of total level for AMH was 4. 55(0. 41~11. 65)ng/ml which will increase in inverse proportion to the age(r=—0. 365,P=0. 000). That was to say,as they grew older, the AMH level would reduce. Conclusion The medical reference range of AMH level in this thesis could be applied to health and medicine practice for clinical diagnosis and treatment of reproduction and infertility of women.%目的 通过测定邯郸地区健康育龄妇女血清抗苗勒氏管激素(AMH)水平及与生殖年龄的相关性,建立该地区实验室血清AMH参考值范围.方法 采用酶联免疫吸附技术(ELISA)检测165例20~50岁健康育龄妇女早卵泡期(月经周期2~5天)或黄体中期(BBT上升后4~8天)血清AMH水平,并同时检测LH,FSH,T,E2,PRL和P等六项生殖激素水平.结果 血清AMH水平呈非正态分布,故参考值范围用中位

  17. 多囊卵巢综合征患者控制性超促排卵过程 AMH 和 INHB 浓度变化的临床研究%Changes of anti-Mullerian hormone (AMH) and inhib in B (INHB) during controlled o varian hyperstimulation in polycystic ovarian syndrome

    李莹; 聂明月; 方颖; 杨晓葵


    目的:探讨多囊卵巢综合征( polycystic ovarian syndrome , PCOS )患者控制性超促排卵( controlled ovarian hyperstimulation,COH)过程中血清抗苗勒管激素(anti-Müllerian hormone,AMH)和抑制素B(inhibin B,INHB)浓度的变化及其对COH结局的预测价值。方法 PCOS 组患者37例,对照组患者41例。检测患者月经周期第2/3天( D2/3)、促性腺激素(gonadotrophin,Gn)刺激第5天(D5)、人绒毛膜促性腺激素(human chorionic gonadotropin,hCG)日(D-hCG)血清AMH及INHB浓度及取卵日卵泡液中AMH及INHB浓度,观察其动态变化及其对COH结局的影响。结果在COH过程中,血清AMH浓度逐渐降低,而血清INHB浓度逐渐升高。 PCOS组患者hCG日血清雌二醇( estrodiol,E2)浓度、获卵数、冷冻胚胎数较对照组明显升高(P<0.05,P<0.001),而Gn用量、受精率均低于对照组,差异有统计学意义(P<0.05),2组患者的临床妊娠率比较,差异无统计学意义(P>0.05)。血清AMH和INHB浓度与Gn剂量、hCG日E 2浓度具有相关性(P<0.05,P<0.001),但是与获卵数、临床妊娠率无相关性(P>0.05)。结论 PCOS患者 AMH和INHB浓度在COH过程中呈现动态变化,可以预测COH中卵巢的反应性,但是对超促排卵结局的预测价值还有待进一步研究。%Objective To explore dynamics of anti-Müllerian hormone ( AMH ) and inhibin B ( INHB ) during controlled ovarian hyperstimulation ( COH) in polycystic ovarian syndrome ( PCOS ) , and assess the value of these dynamic changes on the prediction of outcome in PCOS patients undergoing in vitro fertilization ( IVF) .Methods Thirty-seven women with PCOS and 41 women with normal ovarian reserve as the control were recruited in the study .AMH and INHB levels were measured in serum on menstrual cycle day 2 or 3 (D2/3), day 5 of gonadotropin stimulation(D5), human chorionic

  18. Anti-mullerian hormone as a diagnostic and prognostic tool for PCOS patients

    Wiweko, Budi; Maidarti, Mila; Priangga, M. Dwi; Shafira, Nadia; Fernando, Darrell; Sumapraja, Kanadi; Natadisastra, Muharam; Hestiantoro, Andon


    Purpose To determine whether the measurement of serum AMH can be used to diagnose PCOS and as a tool to predict the prognosis of PCOS. Methods This is a case–control study. Women of reproductive age (18–35 years) were recruited consecutively at a tertiary academic hospital during the period of March 2009–October 2011 and were divided into case (PCOS patients defined by the Rotterdam criteria) and control groups (non-PCOS patients). Menstrual history, clinical manifestations of hyperandrogenis...

  19. Comparison of anti-mullerian hormone and antral follicle count for assessment of ovarian reserve

    Sonal Panchal


    Full Text Available Aim: This study aims to compare the efficacy of AFC and AMH, as markers for ovarian reserve. Materials and Methods: 75 patients with PCO (polycystic ovaries undergoing IVF were randomized with 75 non-PCO patients. On day 3, volume of ovary was acquired, ovarian volume was defined by VOCAL, and Sono AVC was used to count the number of antral follicles. Sum total of antral follicles in both ovaries was taken as total antral follicle count (AFC. AMH was measured on the same day. Long agonist protocol with recombinant FSH (rFSH was used for IVF stimulation till at least two follicles of 18 mm were seen. hCG 10,000 iu was given and ovum pick up was done after 34-35 h. Primary end point was number of follicles >12 mm seen on day of hCG. Final end point was number of ova retrieved on ovum pick up. Correlation of AFC and AMH was checked for both end points and with each other. Results: Correlation of AFC and follicles >12 mm on day of hCG in PCO group is 0.56 and non-PCO group is 0.63, 1 and for AMH and follicles >12 mm on day of hCG in PCO group is 0.42 and non-PCO group is 0.47. Correlation of AFC with number of ova retrieved on OPU in PCO group is 0.44 and for non-PCO group is 0.50. The value for AMH is 0.39 in PCO and 0.43 for non-PCO group. Comparing correlation of AFC and AMH for primary end point in PCO group has ′z′ value 1.11(onetailed significance 0.1335, twotailed significance 0.267 and in non-PCO group comparison shows a ′z′ value of 1.39 (one tailed significance 0.0823, two-tailed significance 0.1645. Therefore in both groups, AFC and AMH correlates with total number of follicles >12 mm on day of hCG, but both AFC and AMH have independent significance. Comparing correlation of AFC and AMH with number of ova retrieved on OPU, in non-PCO group has ′z′ value of 0.54(one tailed 0.2946, two-tailed 0. 5892. In PCO group, this comparison shows, ′z′ value of 0.36(one tailed 0.3594, two tailed 0.7188. Conclusion: AFC and AMH correlate with each other but have independent significance for estimating follicles >12 mm on day of hCG. For ova retrieved on OPU, in PCO group, AMH and AFC have similar significance. In non-PCO, AMH may increase accuracy of estimation of number of ova retrieved on OPU over AFC, but difference is not significant. AFC only may suffice for estimation of number of ova retrieved on OPU for all practical purposes.

  20. The physiology and clinical utility of anti-Mullerian hormone in women

    Dewailly, Didier; Andersen, Claus Yding; Balen, Adam;


    hyperstimulation of the ovaries for IVF and the timing of menopause, and to indicate iatrogenic damage to the ovarian follicle reserve. It has also been proposed as a surrogate for antral follicle count (AFC) in the diagnosis of polycystic ovary syndrome (PCOS). METHODS This paper is a summary of presentations at...... a European Society of Human Reproduction and Embryology campus workshop on AMH, with literature cited until September 2013. Published peer-reviewed medical literature about AMH was searched through MEDLINE and was subjected to systematic review and critical assessment by the panel of authors....... RESULTS Physiologically, recent data confirm that AMH is a follicular gatekeeper limiting follicle growth initiation, and subsequently estradiol production from small antral follicles prior to selection. AMH assays continue to evolve and technical issues remain; the absence of an international standard is...

  1. Research progress of anti-mullerian hormone and follicular development%抗苗勒管激素与卵泡发育的研究进展

    王丽; 吕淑兰



  2. 抗苗勒氏管激素对精子发生的影响%Influence of anti-mullerian hormone on spermatogenesis

    赵海波; 宋晖; 于月成


    目的 检测人精浆中是否存在抗苗勒氏管激素(AMH),并分析其与精子密度、活动率及活力的关系.方法 酶联免疫吸附试验(ELISA)检测150对不孕症患者男方精浆AMH水平,精液分析异常者治疗后再次检测AMH,观察治疗前后精浆AMH水平的变化.结果 精浆中存在AMH,明显高于血清中水平(P<0.01),精液分析正常者精浆AMH水平高于精液分析异常者(P<0.01),AMH水平与精子密度及活动率呈正相关.结论 精液中存在AMH,AMH对精子密度及活动率有促进作用.

  3. Relationship between polymorphism of anti-mullerian hormone,anti-mullerian hormone receptor type Ⅱ and polycystic ovary syndrome%抗苗勒氏管激素及其Ⅱ型受体的基因多态性与多囊卵巢综合征发病的关系

    李键; 乔杰; 闫丽盈; 唐荣欣; 甄秀梅


    目的 探讨抗苗勒氏管激素(AMH)Ile49 Ser及抗苗勒氏管激素Ⅱ型受体(AMHR2)-482A>G多态性与多囊卵巢综合征(PCOS)发病的相关性.方法 采用聚合酶链反应(PCR)和DNA测序方法,检测103例PCOS妇女和79例正常排卵妇女AMHIle49 Ser和AMHR2-482A>G的基因型分布,并采用酶放大化学发光及酶联免疫吸附试验(ELISA)等方法测定血清激素.结果 PCOS组和正常对照组的AMHIle49 Ser和AMHR2-482A>G的基因型分布比较,差异无显著性(P>0.05);PCOS组AMH的Ile/Ile、Ile/Ser和Ser/Ser3种基因型的血清AMH水平分别是(11.0±4.2)、(13.4±6.9)和(17.6±6.5)ng/ml,差异有显著性(PG多态性与PCOS发病可能不相关,PCOS组血清AMH水平明显高于正常排卵妇女,AMHIle49 Ser的多态性可能足影响PCOS妇女卵泡期AMH水平的因素之一.

  4. A New Predictor AMH (anti-müllerian hormone to Determining Ovarian Reserve and Menoposual Aging in the Women

    Levent Sarýyýldýz


    Full Text Available       Anti-mullerian hormone (AMH, also termed Mullerian inhibiting substance (MIS, is produced in ovary by granulose cells and known as a member of the TGF- β superfamily. The researchers imply that AMH is associated with the various growth factors in development and growth of ovarian follicles, furthermore  is exhibited the features of other TGF-β family members. AMH has a critical importance in reproductive age of women. Certain standardization has not yet been developed in determing the ovarian reserve. Recent findings reveal the importance of AMH in determining ovary function and, highlight the importance of AMH than FSH.

  5. Hormones

    Hormones are your body's chemical messengers. They travel in your bloodstream to tissues or organs. They work ... glands, which are special groups of cells, make hormones. The major endocrine glands are the pituitary, pineal, ...

  6. The diversity of anti-mullerian hormone level between different ovarian reserve female%抗苗勒管激素水平在不同卵巢储备功能女性之间的差异

    赵玲; 肖春辉


    目的 探讨抗苗勒管激素(AMH)在多囊卵巢综合征(PCOS)、卵巢早衰(POF)、正常人群中的差异.方法 内分泌门诊就诊的PCOS患者(PCOS组)60例,卵巢早衰(POF)患者(POF组)30例,随机选择健康志愿者30例作为对照组.统计3组年龄,并计算体质量指数(AMI),测定卵泡数量(AFC)、血清(FSH)、黄体生成素(LH)、睾酮(T)、雌三醇(E2)和血清AMH水平.结果 PCOS组AFC明显高于POF组及对照组,对照组明显高于POF组,差异有统计学意义(P<0.01);PCOS组BMI明显高于POF组及对照组,差异有统计学意义(P<0.01);PCOS组T、E2、AMH明显高于POF组与对照组,对照组明显高于POF组,差异有统计学意义(P<0.01);POF组LH水平高于PCOS组和对照组,PCOS组高于对照组,差异均有统计学意义(P<0.01);POF组FSH明显高于PCOS组与对照组,差异有统计学意义(P<0.01).结论 AMH在PCOS、POF、及正常生育年龄女性间存在显著差异,可作为临床评估卵巢储备功能的指标.

  7. 两种药物促排卵过程中PCOS患者AMH变化%Anti-Mullerian Hormone Plasma Levels during Treatment with Two Different Drugs to Induce Ovulation in Women with Polycystic Ovary Syndrome

    田玉燕; 李晓丹; 徐素欣


    目的:探讨来曲唑(LE)和尿促性腺激素(HMG)治疗多囊卵巢综合征(PCOS)患者在促排卵过程中抗苗勒氏管激素(AMH)的变化.方法:选择PCOS患者50例,随机分为2组,分别给予来曲唑2.5 mg/d口服,共5 d;或低剂量递增法肌注hMG;对照组50例;阴道超声计数小卵泡数和优势卵泡数,测定月经第3天、第8天以及hCG日(对照组LH峰日)血AMH、E2.结果:在促排卵过程中,hMG组AMH水平逐渐降低,与E2升高间成负相关(r=0.65);LE组AMH水平略有下降但无显著性;对照组AMH值无明显变化.结论:LE治疗过程中PCOS患者AMH变化及卵泡生长更接近自然月经周期.

  8. Relationship study between anti-mullerian hormone level in human follicular fluid and oocyte%人卵泡液中抗苗勒管激素水平与卵母细胞关系的研究

    赵海波; 宋晖; 于月成


    目的 探讨卵泡液中抗苗勒管激素(AMH)水平对卵母细胞发育、受精和卵裂的影响.方法 采用酶联免疫吸附法(ELISA)检测60例体外受精-胚胎移植患者卵泡液中AMH水平,分析其与卵母细胞发育、受精和卵裂的关系.结果 卵泡液AMH水平为(56.7±11.2)pmol/L,显著高于血清AMH水平((20.7±5.5)pmol/L,P<0.01).含成熟卵母细胞的卵泡液AMH水平为(57.3±12.1)pmol/L,高于含未成熟卵母细胞者((55.8±9.3)pmol/L,P<0.05);受精卵的AMH水平((57.9±11.7)pmol/L)高于未受精卵((54.5±6.6)pmol/L,P<0.01);Ⅰ级和Ⅱ级胚胎的AMH水平高于Ⅲ级和Ⅳ级胚胎(P均<0.05).结论 卵泡液中AMH可促进卵母细胞发育,提高受精卵发育潜能.

  9. 抗苗勒氏管激素早期预测卵巢储备功能的研究%Studies of anti-mullerian hormone on early prediction of the ovarian reserve functions

    王瑜; 韩玉芬; 张晨静; 王志勇



  10. Prediction of Ovarian Reserve with Anti-mullerian Hormone, Inhibin B and Ultrasound%抗苗勒管激素、抑制素B及超声预测卵巢储备功能

    石洁; 张云山




    左建新; 任立盛; 邵翠华; 刘美新; 王言奎


    目的 通过血清抗苗勒管激素(AMH)的测定,评价双侧子宫动脉栓塞术对卵巢储备功能的影响.方法 采用Beckman Coulter ELISA法检测20例宫颈妊娠病人双侧子宫动脉栓塞术前及术后1、6个月血清AMH水平,并比较其变化.结果 宫颈妊娠病人子宫动脉栓塞术前至术后1、6个月血清AMH水平分别为(7.99±4.15)、(7.11±3.02)和(8.33±4.87)mg/L,手术前后比较差异无显著性(F=0.93,P>0.05).结论 子宫动脉栓塞术治疗宫颈妊娠不影响卵巢储备功能.

  12. Induction of WNT inhibitory factor 1 expression by Müllerian inhibiting substance/AntiMullerian hormone in the Müllerian duct mesenchyme is linked to Müllerian duct regression

    Park, Joo Hyun; Tanaka, Yoshihiro; Arango, Nelson A.; Zhang, Lihua; Benedict, L. Andrew; Roh, Mi In; Donahoe, Patricia K.; Teixeira, Jose M.


    A key event during mammalian sexual development is regression of the Müllerian ducts (MDs) in the bipotential urogenital ridges (UGRs) of fetal males, which is caused by the expression of Müllerian inhibiting substance (MIS) in the Sertoli cells of the differentiating testes. The paracrine signaling mechanisms involved in MD regression are not completely understood, particularly since the receptor for MIS, MISR2, is expressed in the mesenchyme surrounding the MD, but regression occurs in both...

  13. The relationship between anti-mullerian hormone and PCOS%抗苗勒管激素与多囊卵巢综合征相关性分析

    李琳; 陈晓莉; 陈亚肖; 李予; 钟俊敏; 杨冬梓


    目的 研究多囊卵巢综合征(PCOS)血清抗苗勒管激素(AMH)水平及其与PCOS各项指标的相关性.方法 选择2006年6~12月在中山大学附属第二医院妇产科就诊的47例PCOS患者为研究组,收集同期年龄匹配的40例健康女性为对照组,应用酶联免疫吸附(ELISA)法检测血清AMH.结果 研究组的血清AMH明显高于对照组[(9.85±4.93)ug/L对(7.13±3.02)ug/L,P=0.002).成年期研究组血清AMH亦明显高于对照组[(10.64±5.47)ug/L对(7.01±2.43)ug/L,P=0.005),而青春期研究组血清AMH虽较对照组增高,但差异无统计学意义[(8.95±4.18)ug/L对(7.26±3.58)ug/L,P=0.168).Pearson相关分析显示血清AMH与年龄无相关性,与雄激素和其他内分泌等各项指标无相关性,与平均卵巢体积呈正相关(r=0.319,P=0.029),但与平均卵泡数目无相关性.结论 PCOS患者的血清AMH明显增高,其与血清雄激素相关性不明显,提示AMH有可能通过某种机制在卵泡局部发挥作用,影响卵泡发育而参与PCOS的病理生理过程.

  14. 抗苗勒氏管激素与多囊卵巢综合征的相关性研究%Study on the relationship between serum anti-mullerian hormone and polycystic ovary syndrome

    董晨; 李红发


    目的 探讨抗苗勒氏管激素(AMH)与多囊卵巢综合征(PCOS)发生、发展的关系.方法 测定51例PCOS患者血清AMH、卵泡刺激素、黄体生成素、催乳素、睾酮、雌二醇水平以及双侧卵巢卵泡数(FN),并取20例月经周期正常妇女进行对照.结果 PCOS组血清AMH(31.10±13.44)pmol/L,明显高于对照组(12.01±4.44)pmol/L,差异有极显著性,并且两组血清AMH水平都与FN呈正相关.除此之外,PCOS患者血清AMH水平还与睾酮水平呈显著正相关,且这种相关关系并不出现在对照组.结论 AMH可能参与PCOS卵泡发育异常并与性激素合成失调有关.测定血清AMH水平为诊断和研究PCOS提供了一个重要的突破点.

  15. 血清抗苗勒管激素在预测围绝经期女性卵巢功能中的应用%Application of anti-mullerian hormone serum in predicting ovarian functions of perimenopausal women

    杨冰; 叶元; 秦辛玲; 石青峰


    目的 观察血清抗苗勒管激素(AMH)在围绝经期女性中的变化,探讨AMH用于评估卵巢功能的意义.方法 选取月经失调患者60例,根据卵巢功能分为围绝经期组和卵巢早衰组(POF组),各30例;选取同期月经周期正常、无内分泌疾病、年龄在35 ~ 45岁的健康妇女30例,作为对照组.分别于就诊当月或下月月经周期第2~3天(闭经者日期不限),检测各组血清AMH、卵泡刺激素(FSH)、黄体生成激素(LH)、雌二醇(E2)水平,其中围绝经期组6、12个月之后重复检测上述项目.结果 与对照组比较,POF组血清AMH、E2水平下降,FSH、LH水平上升(P均<0.05);与对照组比较,围绝经期组血清AMH下降(P<0.05),FSH水平稍上升,LH、E2在正常水平(P均>0.05);POF组血清AMH水平低于围绝经期组(P<0.05);围绝经期组6个月后重复检测,血清AMH水平较就诊时下降(P<0.05),而FSH、LH、E2水平变化不明显(P均>0.05);12个月后,血清AMH水平进一步下降,FSH、LH水平上升,E2水平下降,P均<0.05.结论 血清AMH比FSH、LH、E2更早反映卵巢功能下降,能更早预测围绝经期妇女卵巢功能衰退.

  16. 抗苗勒管激素与多囊卵巢不孕患者有无排卵的相关关系%Relationship between serum anti - mullerian hormone and ovulation in women with polycystic ovaries

    李键; 乔杰; 赵淑云; 王静; 严杰; 李蓉


    目的 研究抗苗勒管激素(AMH)与多囊卵巢(PCO)不孕患者有无排卵的关系,探讨AMH在卵巢卵泡生长和发育停滞中的作用.方法 采用酶联免疫吸附法(ELISA)检测35例正常卵巢形态的排卵妇女(A组)、29例PCO伴正常排卵妇女(B组)和38例PCO伴排卵障碍妇女(C组)的月经第2 d(d2)血清及排卵前卵泡液(FF)的AMH水平.结果 B组和C组的月经d2血清AMH水平比较.差异无显著性(5.87ng/ml和7.99 ng/ml;P>0.05),但显著高于A组(2.52 ng/ml;P0.05),但显著高于A组(29.45 ng/g pro;P<0.05).结论 多囊卵巢患者血清AMH水平升高,其颗粒细胞可能存在AMH分泌异常,但多囊卵巢患者AMH水平的增高可能不是造成卵泡生长停滞的原因.

  17. Prediction of assisted reproductive technique outcome in elevated early follicular phase follicle stimulating hormone with Mullerian inhibiting substance level

    Leili Safdarian


    Full Text Available Background: Detection of best predictor of ovarian reserve in patients with temporarily or consistently elevated early follicular phase serum levels of FSH is one of the most important goals in assisted reproductive technique (ART.Objective: To evaluate whether high level of anti-mullerian hormone level is related to success of ART in patients with temporarily or consistently elevated early follicular phase serum levels of FSH.Materials and Methods: Sixty three women underwent intracytoplasmic sperm injection (ICSI with GnRH-agonist long protocol or intrauterine insemination (IUI in a prospective cohort study. FSH, inhibin B and anti-Mullerian hormone (AMH levels were measured in these women whom were divided to three groups (persistently elevated FSH, variably elevated FSH and, normal FSH level. Basal characteristics, stimulation parameters, and pregnancy occurrence were evaluated.Results: AMH was significantly higher in women with persistently elevated early follicular phase FSH achieving pregnancy. Women with normal FSH did not have significant difference in AMH level between conceived and non conceived cycles. Women with only one elevated early follicular phase FSH achieving pregnancy did not have significant difference in AMH level with non pregnant women. Response to gonadotropin stimulation, recommendation to oocyte donation significantly differed between the groups.Conclusion: This study has demonstrated that relatively young women with persistently or intermittently elevated day 3 FSH levels have diminished ovarian reserve and lower ART success. However, in women whose FSH levels were constantly elevated, AMH (not inhibin B concentrations were significantly higher in ART cycles resulting in pregnancy. Therefore, AMH level is a good predictor of ART outcome in patients with elevated early follicular phase serum levels of FSH.

  18. Naturopathy and yoga in ameliorating multiple hormonal imbalance: a single case report

    Pradeep M.K. Nair


    Full Text Available Hormonal imbalances are common among the women who are in the halfway of their reproductive age. There are lot of factors like stress, diet, lifestyle etc. which contributes to this hormonal dysfunctions. However these factors are merely addressed by the existing management strategies. A 37 year old female presented with hypothyroidism and associated hyperprolactinemia had undergone Naturopathy and yoga interventions for a period of 18 months. Her baseline TSH and prolactin levels were 9.2 U/ml and 34 ng/ml respectively. Her anti-mullerian hormone (AMH levels also fell down to 0.3 ng/ml. Naturopathy and yoga based lifestyle interventions including hydrotherapy, mud therapy, yoga therapy and acupuncture were given for different durations has streamlined the hormone levels to normal range (TSH-4.6 U/ml, prolactin- 19.6 ng/ml, AMH-2.6 ng/ml. The results indicate that naturopathy and yoga has a positive role in reinstating the hormonal homeostasis. However large scale studies are warranted to bestow better care. [Int J Reprod Contracept Obstet Gynecol 2016; 5(3.000: 916-918

  19. 促性腺激素释放激素拮抗剂对卵巢颗粒细胞分泌抗苗勒管激素的影响%Effect of gonadotropin releasing hormone antagonist on the secretion of anti-Mullerian hormone of granulosa cells

    张英; 陆芝英; 王婷; 王佳佳; 华克勤


    目的:研究促性腺激素释放激素拮抗剂(GnRH-ant)对卵巢颗粒细胞分泌抗苗勒管激素(AMH)的影响.方法:从人卵泡液中分离、培养颗粒细胞.分别用生理盐水(NS)、顺铂(cisplatin,DDP,5μg/ml)、cetrorelix(GnRH-ant,10-9mol/L)或cetrorelix (10-9mol/L)+DDP(5μg/ml)处理颗粒细胞24 h后,ELISA方法检测颗粒细胞分泌AMH的水平.裸鼠动物随机分为4组:NS组:皮下注射NS0.1 ml/天;1周后腹腔注射NS 0.2 ml/周.DDP组:皮下注射NS 0.1 ml/天;1周后腹腔注射5 mg/kg DDP/周.cetrorelix组:皮下注射100 μg cetrorelix/天;1周后腹腔注射NS 0.2 ml/周.cetrorelix+DDP组:皮下注射100 μg cetrorelix/天;1周后腹腔注射5 mg/kg DDP/周.用药第29天,经眼球取血后用ELISA方法检测各组裸鼠血清AMH的水平.结果:成功分离、培养人卵巢颗粒细胞.与DDP组及NS组比较,cetrorelix组及cetrorelix+ DDP组颗粒细胞AMH分泌明显增加(P<0.05).Cetrorelix组及cetrorelix+ DDP组裸鼠血清AMH水平明显高于DDP组(P<0.05).结论:GnRH-ant能够上调颗粒细胞分泌AMH,对抗DDP对AMH的降调节作用.

  20. Correlation of anti-Mullerian hormone and baseline follicle-stimulating hormone levels in women with regular menstruation%月经规律女性抗缪勒氏管激素与基础卵泡刺激素关系的研究

    宁宁; 张杰; 王社教; 尉春艳; 陈庆; 何长武


    目的 探讨月经规律女性抗缪勒氏管激素(AMH)随年龄变化规律及其与基础卵泡刺激素(bFSH)之间的关系.方法 以89例月经规律女性为研究对象,按年龄分为5组(20 ~24岁组,25 ~29岁组,30 ~ 34岁组,35 ~ 39岁组,40~44岁组),应用全自动电化学发光法测定血清中AMH及bFSH水平,分析AMH及bFSH随年龄的变化规律及其相互间的关系.结果 AMH水平与年龄呈负相关(r=-0.847,P<0.001);bFSH与年龄呈正相关(r=0.296,P=0.005);除第2、3组间AMH无明显差异外(P=0.091),其余各组间均有显著性差异(P1∶2<0.001,P1∶3<0.001,P1∶4<0.001,P1∶5<0.001,P2∶4<0.001,P2∶5<0.001,P3∶4 =0.003,P3∶5 <0.001,P4∶5<0.001);血清AMH水平与bFSH呈负相关(r=-0.406,P<0.05).结论 月经规律女性AMH水平随年龄增加逐渐下降,其与年龄相关性优于bFSH,且AMH水平可预测bFSH水平,在评价卵巢功能中起重要作用.

  1. 血清抗苗勒管激素水平与多囊卵巢综合征激素特征关系的研究%Relationship between the level of serum anti - mullerian hormone and hormone characteristics in polycystic ovary syndrome

    孙静; 熊晓英; 齐国华; 王颖; 张树芝; 胡舒勤; 孟军; 赖雪娜


    目的 探讨抗苗勒管激素(AMH)与多囊卵巢综合征(PCOS)卵泡发育和激素特征的关系.方法 测定47例Pcos患者血清AMH、卵泡刺激素、黄体生成素、催乳素、睾酮、雌二醇水平以及双侧卵巢卵泡数(FN),并取23例月经周期正常妇女进行对照.结果 PCOS组血清AMH(32.57±13.31)pmol/L、T(3.01±0.12)nmol/L、LH(17.68±3.11)U/L水平,明显高于对照组AMH(13.06±4.59)pmol/L、T(1.31±0.02)nmol/L、LH(9.07±2.09)U/L,两组差异有统计学意义(P<0.01),并且两组血清AMH水平都与FN呈正相关.结论 血清AMH异常可能是PCOS卵泡发育异常和性激素合成失调的原因之一;测定血清AMH水平为诊断和研究PCOS提供了一个重要的方法.

  2. The expression of serum anti-mullerian hormone Serum in different crowd and its correlation research%抗苗勒管激素在卵巢储备功能不同患者中的表达及相关性研究

    田春花; 胡蓉; 罗艳; 李彩艳


    目的 通过测定卵巢早衰(POF)、多囊卵巢综合征(PCOS)及正常对照组患者血清中抗苗勒管激素(AMH)水平,探讨血清AMH在不同卵巢储备功能患者中的不同表达.方法 测定POF组(25例)、PCOS组(32例)和正常对照组(30例)血清中AMH水平,同时测定上述三组人群血清中卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)、睾酮(T)及泌乳素(PRL)水平,并记录受试者的年龄、体重指数(MBI)、空腹血糖及胰岛素水平.结果 ①三组中POF血清AMH值最低,PLOS最高,且差异有统计学意义(P<0.05);②三组血清中FSH、LH、PRL、E2 、T及AMH水平差异均有统计学意义(P<0.05),而这三组间年龄、MBI差异无统计学意义(P>0.05);③PCOS组血清中AMH与LH、T及MBI呈正相关性 (P<0.05),与FSH和胰岛素抵抗指数无相关性.结论 在不同卵巢储备功能患者中血清AMH表达不同,可能参与PCOS发生、发展,并与性激素合成失调有关.

  3. 1400名0~5岁健康儿童血清抗苗勒氏管激素及抑制素B临床检测研究%A clinical study on the detection of serum anti-Mullerian hormone and inhibin B in 1400 cases of healthy children aged 0 to 5 years


    目的 检测0~5岁健康儿童血清抗苗勒氏管激素(AMH)及抑制素B(INHB)浓度,为建立正常参考范围提供帮助.方法 选择来自上海市儿童医院、深圳市儿童医院、天津医科大学总医院、江西省儿童医院及成都市妇女儿童中心医院的健康体检男童及女童各700名,共1400名.根据年龄分为7组:0~1个月;>1~6个月;>6个月至1岁;>1~2岁;>2~3岁;>3~4岁;>4~5岁,其中每组男童及女童各100名.采用酶联免疫吸附法(ELISA)检测血清AMH浓度(μg/L)及INHB浓度(ng/L).结果 0~5岁健康男童血清AMH浓度在>1~6个月达高峰210.70(55.37~439.45) μg/L,>1~5岁维持平稳低水平147.10(17.69 ~ 321.88) ng/L;女童血清AMH浓度在>1~6个月也出现小高峰1.77 (0.06 ~ 7.68) ng/L,>2~5岁趋于平稳为1.98(0.19 ~ 7.31) ng/L.男童血清INHB浓度在>1~6个月达高峰274.23(0.00~602.34) ng/L,女童>1~6个月出现小高峰为36.83(0.00~226.32) ng/L.结论 提供了多中心及大样本0~5岁健康儿童血清AMH及INHB值的浓度范围,并发现>1~6个月年龄段出现浓度高峰.

  4. Analysis on anti-mullerian hormone, antiendometrial antibody and anti-ovary antibody of female patients with infertility%女性不孕患者抗苗勒管激素、抗子宫内膜抗体和抗卵巢抗体检测分析

    马芳芳; 王厚照


    目的 探讨抗苗勒管激素(AMH)、抗卵巢抗体(AOAb)、抗子宫内膜抗体(AEAb)女性不孕的关系方法 采用酶联免疫吸附实验 ELISA 和化学发光法对 800 例女性不孕患者和850例有生育史妇女进行AMH、AOAb和AEAb检测.结果 女性不孕患者抗苗勒管激素AMH水平为6.01±1.17 ng/mL,明显高于对照组(P<0.05);抗子宫内膜抗体EMAb阳性率为10.25%,抗卵巢抗体AOAb阳性率为6.38%,阳性率都明显高于对照组(P<0.05).结论 AMH、AOAb和AEAb与女性不孕密切相关,对临床诊断和治疗具有重要的指导意义.

  5. Value of serum anti-Mullerian hormone measurement in predicting ovarian response in controlled ovarian hyperstimulation%血清抗苗勒氏管激素在超促排卵周期中预测卵巢反应性的临床研究

    刘阳; 蔡霞; 杜红; 玛依热·吐尔逊


    目的 探讨血清抗苗勒氏管激素水平(AMH)在辅助生殖超排卵周期中预测卵巢反应性的价值.方法 按获卵数及人绒毛膜促性腺激素(hCG)注射日雌二醇(E2)水平,将68例接受体外受精-胚胎移植(IVF-ET)/体外受精-胞浆内单精子注射(ICSl)的不孕症患者分为卵巢低反应组(A组)、卵巢正常反应组(B组)及卵巢高反应组(C组).于促排周期月经第2天及hCG注射日,分别检测血清AMH、E2及孕酮(P)水平.比较3组临床效果及注射rFSH后AMH、E2、黄体生成素(LH)及P水平的变化.按妊娠结局分为妊娠组和非妊娠组,比较AMH、窦卵泡数(AFC)、优质胚胎率、优质胚胎数的变化.结果 ①A、B、C 3组月经第2天(D2)AMH水平的M分别为0.645、1.762及3.101 ng/mL;IVF周期hCG日AMH水平M分别为0.331、0.898及1.434 ng/mL,3组比较差异有统计学意义(P<0.001);多重比较后均发现D2AMH及hCG日AMH差异有统计学意义(P<0.001)(C组>B组>A组).②获卵数与促排周期D2AMH、hCG日AMH、AFC(窦卵泡数)、P呈显著正相关;与促排周期D2卵泡刺激(FSH)、促性腺激素(GN)量呈负相关.③卵巢过度刺激综合征(OHSS)的发生和GN量、AFC、D2AMH、HCG日E2及HCG日AMH水平均成正相关;与D2FSH成负相关.④妊娠组和未妊娠组间的卵裂率、优胚率及优质胚胎数间有差异性(P<0.05).结论 ①D2AMH、hCG日的AMH水平及AFC均是预测卵巢反应性较好的标记物;②D2AMH、hCG日AMH及hCG日E2水平对预测OHSS的发生具有一定的指导意义;③AMH水平不能预测妊娠结局.

  6. Impact of laparoscopic ovarian drilling on anti-Mullerian hormone levels in women with polycystic ovary syndrome%腹腔镜卵巢打孔术对多囊卵巢综合征患者抗苗勒激素水平的影响



    目的 评估腹腔镜卵巢打孔术(LOD)对多囊卵巢综合征(PCOS)患者抗苗勒激素(AMH)水平的影响.方法 对35位PCOS妇女行腹腔镜卵巢打孔术,并观察血清抗苗勒激素水平和妊娠率.结果 PCOS组患者血清AMH是正常对照组妇女的4.6倍.妊娠PCOS患者LOD前后AMH水平降低36%.结论 检测LOD治疗的PCOS妇女的血清AMH水平,将有助于预测LOD结局.

  7. Prediction value of serum anti-Mullerian hormone on ovarian response in controlled ovarian hyperstimulation in IVF%血清抗苗勒氏管激素在体外受精超排卵中预测卵巢反应的价值

    王馥新; 杨辰; 吴惠华; 邹琴燕; 孟庆霞; 许咏乐; 王玮; 李红


    目的 血清抗苗勒管激素(AMH)由窦前和小窦卵泡分泌,是一个反映卵巢储备的指标.分析AMH在正常育龄人群、多囊卵巢综合征(PCOS)患者、卵巢储备功能减退(DOR)人群中的分布规律,探讨AMH预测卵巢反应的价值. 方法 回顾分析2008年6月至2012年6月在我中心接受诊治的患者,将其分为三组:(1)正常组:293例.(2) PCOS组:258例.(3)DOR组:59例.比较AMH水平在这三组间的差异,绘制ROC曲线选择判断PCOS及DOR的最佳临界值.进一步选取同时间段,在我中心初次行长方案体外受精/卵胞浆内单精子注射-胚胎移植(IVF/ICSI-ET)的患者,578例.按获卵数分为卵巢低反应组(0~3个)、正常反应组(4~19个)、高反应组(≥20个),评估年龄、基础窦卵泡计数(AFC)、基础卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)水平,以及AMH、促性腺激素(Gn)用量与卵巢反应的关系.绘制相对工作特征(ROC)曲线,寻找AMH在IVF中判断卵巢反应的界点. 结果 AMH水平随年龄增加而下降.AMH水平在PCOS组中较正常组显著升高,在DOR组中明显降低.25~40岁人群中,当AMH界值取1.26 ng/ml时,判断DOR的敏感性及特异性分别为88%及90%.当AMH界值取3.97 ng/ml时,判断PCOS的敏感性及特异性分别为74%及63%.在IVF中,AFC和AMH水平在三组间均有显著差异(P<0.05).当AMH界值为1.84 ng/ml时,判断该患者本周期为低反应的敏感性及特异性分别为89%及75%,当AMH界值为3.95 ng/ml时,判断为高反应的敏感性及特异性为79%及70%. 结论 AMH反映卵巢储备状态.在IVF超排卵中,AMH能较好地预测卵巢反应性.

  8. The correlation between anti-Mullerian hormone and insulin resistance in rats with polycystic ovary syndrome%多囊卵巢综合征大鼠抗苗勒管素与胰岛素抵抗水平相关性分析

    孙秀红; 韦相才


    目的 探讨多囊卵巢综合征(PCOS)大鼠血清及卵巢单卵泡抗苗勒管素(AMH)水平与胰岛素抵抗(IR)水平间的相关性.方法 采用孕激素联合绒毛膜促性腺激素(hCG)方法制作SD大鼠PCOS模型,测定大鼠血清AMH、黄体生成素(LH)、卵泡刺激素(FSH)、空腹血糖(FPG)及空腹胰岛素(FINS),计算LH/FSH、HOMA指数,根据HOMA指数分为PCOS无IR组和PCOS合并IR组,并与对照组分析比较,行相关性和回归分析;用免疫组织化学法对不同组单卵泡AMH水平行半定量分析.结果 实验组血清AMH水平均显著高于对照组(P=0.043),PCOS无IR组和PCOS并IR组血清AMH水平与HOMA指数呈正相关(r=0.691,P=0.001);血清AMH与HOMA指数和FINS之间有直线回归关系(F=12.362,P=0.000);免疫组化显示3组间单卵泡AMH水平差异无统计学意义(P=0.720).结论 PCOS大鼠模型中AMH和胰岛素共同影响卵泡生长发育,AMH和胰岛素水平呈正相关性;卵巢局部单卵泡抗苗勒管素表达水平差异无统计学意义.

  9. Chronic Pelvic Inflammation Diminished Ovarian Reserve as Indicated by Serum Anti Mulerrian Hormone.

    Linlin Cui

    Full Text Available To explore the potential damaging effect of chronic pelvic inflammation on ovarian reserve.Case-control study.A total of 122 women with bilateral tubal occlusion, diagnosed by hysterosalipingography (HSG and 217 women with normal fallopians were recruited.Serum anti-Mullerian hormone (AMH, basic follicle-stimulating hormone (FSH, luteining hormone (LH, estradiol (E2, and testosterone (T were measured; and antral follicle counts (AFCs were recorded.Significantly lower level of AMH was observed in women with bilateral tubal occlusion compared to control group [2.62 (2.95 ng/ml vs. 3.37 (3.11 ng/ml, P = 0.03], and the difference remained after adjustment of BMI (Padjust = 0.04. However, no statistical difference was found in the levels of FSH [7.00 (2.16 IU/L vs. 6.74 (2.30 IU/L], LH [4.18 (1.52 IU/L vs. 4.63 (2.52 IU/L], E2 [35.95 (20.40 pg/ml vs. 34.90 (17.85 pg/ml], T [25.07±11.46 ng/dl vs. 24.84±12.75 ng/dl], and AFC [6.00 (4.00 vs. 7.00 (4.00] between two groups (p>0.05.Women with bilateral tubal occlusion showed decreased AMH level, suggesting that chronic pelvic inflammation may diminish ovarian reserve. More caution should be paid when evaluating the detriment of PID on female fertility.

  10. Does AMH Reflect Follicle Number Similarly in Women with and without PCOS?

    Sverre C Christiansen

    Full Text Available Increased Anti-Mullerian Hormone in polycystic ovary syndrome, may be due to overactive follicles rather than reflect antral follicle count.Does Anti-Mullerian Hormone reflect antral follicle count similarly in women with or without polycystic ovary syndrome or polycystic ovarian morphology?Cross-sectional, case-control.Women who delivered preterm in 1999-2006. For each index woman, a woman with a term delivery was identified.Participation rate was 69%. Between 2006-2008, 262 women were included, and diagnosed to have polycystic ovary syndrome, polycystic ovarian morphology or to be normal controls.Blood tests, a clinical examination and vaginal ultrasound.Anti-Mullerian Hormone/antral follicle count-ratio, SHBG, androstenedione and insulin, to test potential influence on the Anti-Mullerian Hormone/antral follicle count -ratio.Mean Anti-Mullerian Hormone/antral follicle count ratio in women with polycystic ovary syndrome or polycystic ovarian morphology was similar to that of the controls (polycystic ovary syndrome: 1,2 p = 0,10 polycystic ovarian morphology: 1,2, p = 0,27 Controls 1,3. Anti-Mullerian Hormone showed a positive linear correlation to antral follicle count in all groups. Multivariate analysis did not change the results.We confirmed the positive correlation between AMH and follicle count. Anti-Mullerian Hormone seems to be a reliable predictor of antral follicle count, independent of polycystic ovary syndrome diagnosis or ovarian morphology.

  11. Primary Ovarian Insufficiency (POI)

    ... HCP may also check other hormones including an “AMH” (Anti-Mullerian Hormone) level and genetic tests to ... there is a medical reason for the POI. AMH is made by the follicles (within the ovaries). ...

  12. Growth Hormone

    ... page: Was this page helpful? Also known as: GH; Human Growth Hormone; HGH; Somatotropin; Growth Hormone Stimulation Test; Growth Hormone ... I should know? How is it used? Growth hormone (GH) testing is primarily used to identify growth hormone ...


    N. Abdulah


    Full Text Available Background: The age of nulliparous women has increased in developed countries and the 5-year survival rate for the late stage does not exceed 58%. Moreover, as more women are delaying child-bearing, preservation of fertility and reproductive function is a major concern when young women are counseled with regard to the effects of treatment for cervical cancer. Objective: To determine the effect of the combination chemotherapy on serum levels of Anti-Mullerian Hormone (AMH and inhibin B. Methods: This study is a prospective cohort study in 16 cervical cancer patients aged 28-48 years who received Paclitaxel-Cisplatin chemotherapy. AMH and inhibin B levels were examined before and after third series chemotherapy. Statistical analysis used the Wilcoxon test with a level of significance selected at 0.05. Results: There is a significant decrease in median serum levels of AMH and inhibin B patients between before and after Paclitaxel–Cisplatin chemotherapy with p=0.000 (P <0.05. Similarly, a decrease in the median value of serum levels of AMH and inihibin B after Paclitaxel-Cisplatin chemotherapy in each series of chemotherapy was significant with p=0.000 (P <0.05. Conclusions: Serum levels of AMH and inhibin B in cervical cancer patients who received the combination chemotherapy decreased dramatically after 3 months of chemotherapy and the factors that contribute to the diminution is age.

  14. Growth Hormone Ameliorates the Radiotherapy-Induced Ovarian Follicular Loss in Rats: Impact on Oxidative Stress, Apoptosis and IGF-1/IGF-1R Axis.

    Yasmen F Mahran

    Full Text Available Radiotherapy is one of the standard cytotoxic therapies for cancer. However, it has a profound impact on ovarian function leading to premature ovarian failure and infertility. Since none of the currently available methods for fertility preservation guarantees future fertility, the need for an effective radioprotective agent is highly intensified. The present study investigated the mechanisms of the potential radioprotective effect of growth hormone (GH on γ irradiation-induced ovarian failure and the impact of the insulin like growth factor 1 (IGF-1 in the underlying protection. Immature female Sprague-Dawley rats were either exposed to single whole body irradiation (3.2 Gy and/or treated with GH (1 mg/kg s.c. Experimental γ-irradiation produced an array of ovarian dysfunction that was evident by assessment of hormonal changes, follicular development, proliferation marker (PCNA, oxidative stress as well as apoptotic markers. In addition, IGF-1/IGF-1R axis expression was assessed using real-time PCR and immunolocalization techniques. Furthermore, after full maturity, fertility assessment was performed. GH significantly enhanced follicular development and restored anti-Mullerian hormone serum level as compared with the irradiated group. In addition, GH significantly ameliorated the deleterious effects of irradiation on oxidative status, PCNA and apoptosis. Interestingly, GH was shown to enhance the ovarian IGF-1 at transcription and translation levels, a property that contributes significantly to its radioprotective effect. Finally, GH regained the fertility that was lost following irradiation. In conclusion, GH showed a radioprotective effect and rescued the ovarian reserve through increasing local IGF-1 level and counteracting the oxidative stress-mediated apoptosis.

  15. 抗苗勒管激素与女性生殖功能障碍及性早熟'%Evolution of Anti-müllerian Hormone in Female Dysgenesia and Precocious Puberty



    抗苗勒管激素(anti-mullerian hormone,AMH)是转化生长因子β超家族的成员,在女性中仅表达于卵巢的颗粒细胞,可用于评估卵巢的储备功能.其有Ⅰ~Ⅲ型受体,现已明确人类的AMH通过其II型受体(AMHR Ⅱ)发挥作用.目前多囊卵巢综合征(PCOS)、卵巢功能早衰(POF)是引起育龄期妇女不孕的主要原因.AMH在PCOS患者血清中浓度较高;而在POF患者血清中浓度较低,甚至检测不到;在性早熟(PA)患儿血清中的浓度接近正常.研究发现,AMH及AMHR Q的基因多态性与PCOS和POF的发病密切相关.AMH可作为PCOS,POF和PA等疾病的诊断依据,并可指导PCOS患者的临床治疗.

  16. Hormone assay

    An improved radioimmunoassay is described for measuring total triiodothyronine or total thyroxine levels in a sample of serum containing free endogenous thyroid hormone and endogenous thyroid hormone bound to thyroid hormone binding protein. The thyroid hormone is released from the protein by adding hydrochloric acid to the serum. The pH of the separated thyroid hormone and thyroid hormone binding protein is raised in the absence of a blocking agent without interference from the endogenous protein. 125I-labelled thyroid hormone and thyroid hormone antibodies are added to the mixture, allowing the labelled and unlabelled thyroid hormone and the thyroid hormone antibody to bind competitively. This results in free thyroid hormone being separated from antibody bound thyroid hormone and thus the unknown quantity of thyroid hormone may be determined. A thyroid hormone test assay kit is described for this radioimmunoassay. It provides a 'single tube' assay which does not require blocking agents for endogenous protein interference nor an external solid phase sorption step for the separation of bound and free hormone after the competitive binding step; it also requires a minimum number of manipulative steps. Examples of the assay are given to illustrate the reproducibility, linearity and specificity of the assay. (UK)

  17. Bioidentical Hormones and Menopause

    ... Balance › Bioidentical Hormones and Menopause Fact Sheet Bioidentical Hormones and Menopause January, 2012 Download PDFs English Espanol ... take HT for symptom relief.) What are bioidentical hormones? Bioidentical hormones are identical to the hormones that ...

  18. Hormone Data

    National Oceanic and Atmospheric Administration, Department of Commerce — Hormones quantified from marine mammal and sea turtle tissue provide information about the status of each animal sampled, including its sex, reproductive status and...

  19. Hormone impostors

    Colborn, T.; Dumanoski, D.; Myers, J.P.


    This article discusses the accumulating evidence that some synthetic chemicals disrupt hormones in one way or another. Some mimic estrogen and others interfere with other parts of the body`s control or endocrine system such as testosterone and thyroid metabolism. Included are PCBs, dioxins, furans, atrazine, DDT. Several short sidebars highlight areas where there are or have been particular problems.

  20. Endocrine Glands & Their Hormones

    ... Characteristics of Hormones Endocrine Glands & Their Hormones Pituitary & Pineal Glands Thyroid & Parathyroid Glands Adrenal Gland Pancreas Gonads Other ... hormone secretion. « Previous (Characteristics of Hormones) Next (Pituitary & Pineal Glands) » Contact Us | Privacy Policy | Accessibility | FOIA | File Formats ...

  1. 血清抗苗勒管激素水平检测在预测卵巢低反应中的应用价值%Value of serum anti-Müllerian hormone levels for predicting low ovarian response

    廖月婵; 吴日然; 柯玩娜; 林晓丽; 叶云; 杜彦; 杜静; 林秀峰


    Objective: To evaluate the value of serum anti-Mullerian hormone (AMH) in predicting ovarian response to controlled ovarian hyperstimulation (COH) during in vitro fertilization. Methods: A total of 335 COH cycles including 6,5 cycles with poor ovarian response (the number of retrieved oocytes ≤5) and 243 cycles with normal response (the number of retrieved oocytes ≥5) were retrospectively evaluated from January to June, 2010. The serum levels of AMH, FSH, LH and E2 were measured on day 2-5 in spontaneous menstrual cycle of the patients. The patients were received transvagi-nal ultrasound examination, and their antral follicle count (AFC) was assayed. Results: The number of retrieved oocytes was correlated with AMH, AFC, age, FSH/LH, basal FSH levels, basal LH levels, and there is a gradual tendency from strong to weak. The area under the receive operating characteristic curve (ROC) of AMH, AFC, basal FSH levels, FSH/LH, age, basal LH levels, basal E2 levels was from large to small. The cut-off value of anti-Mullerian hormone for predicting ovarian response was equal or more than 2. 21 μg/L with 77% of sensitivity and 80% of specificity. Conclusions: Serum AMH levels might be a better parameter for predicting ovarian response. It could be used as a parameter for selecting the scheme of COH to reduce the cancellation rate of poor responders.%目的 探讨血清抗苗勒管激素(AMH)水平对于卵巢储备功能低下患者在控制性超排卵(COH)中卵巢反应的应用价值. 方法 回顾性分析2010年1月至6月在本中心接受体外受精/卵胞浆内单精子注射-胚胎移植(IVF/ICSI-ET)治疗的308周期.患者分为卵巢低反应组(65例)和卵巢正常反应组(243例).清晨空腹静脉血测定AMH(无月经周期限制)和卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)水平(月经第2~5天),同时行阴道B超检查测定窦卵泡数(AFC),以获卵数为评价标准. 结果 与获卵数的相关性由强到弱是AMH水平

  2. 抗苗勒氏管激素(AMH)对离体人卵巢黄素化颗粒细胞干细胞因子(SCF)表达负调控的研究%Impact of Anti Miillerian Hormone(AMH) on the Expression of Stem Cell Factor (SCF) in Human Granulosa Cells Cultured in Vitro

    胡蓉; 王飞苗; 罗艳; 马会明; 吴昕


    Objective: To explore the influence of anti Mullerian hormone (AMH) on the expression of SCF in human granulosa cells cultured in vitro. Methods: Fifteen patients were recruited who received in vitro fertilization and embryo transfer (IVF-ET) at age 0.05). Conclusion: AMH can effectively down-regulate the expressions of SCF mRNA and protein in human granulosa cells.%目的:研究人卵巢黄素化颗粒细胞中抗苗勒氏管激素(AMH)对干细胞因子(SCF)表达的影响.方法:收集15例年龄<35岁因男方因素行体外受精-胚胎移植(IVF-ET)患者的卵巢颗粒细胞,经原代培养,加入不同浓度基因重组人抗苗勒氏管激素(rhAMH),分别于培养的第4日与第6日采用实时定量PCR(RT-PCR)和免疫组织化学检测空白对照组与各实验组SCF mRNA及蛋白的表达.结果:RT-PCR和免疫组织化学均证实人卵巢黄素化颗粒细胞在不同浓度rhAMH干预后,SCFmRNA及蛋白的表达显著减少(P<0.05),其中15 ng/ml rhAMH抑制作用最明显;实验组第4日与第6日颗粒细胞表达SCFmRNA及蛋白无明显差异(P>0.05).结论:AMH可有效抑制人卵巢黄素化颗粒细胞SCF mRNA及蛋白的表达.

  3. Thymic hormones

    Davies, A.J.S.


    RResults of experiments by various investigators attempting to demonstrate the existence of thymic hormones are reported. In most cases irradiated, thymectomized mice injected with bone marrow cells were used; some experiments were carried out on various extracts of thymuses. Results of most experiments were negative. In one experiment using mice with thymus transplants, sera were evaluated for their capacity to restore azathioprine sensitivity in relation to the rosette forming capacity to spleen cells of thymectomized mice in vitro. In all instances the thymus-grafted mice had a higher titer of serum factor than did normal mice. (HLW)

  4. Thyroid Hormone Treatment

    ... Giving Workplace Giving Other Ways to Donate Thyroid Hormone Treatment Thyroid hormone is used in two situations: ... prevent recurrence or progression of their cancer. THYROID HORMONE REPLACEMENT THERAPY Many people have a thyroid gland ...

  5. Hormone Replacement Therapy

    ... before and during menopause, the levels of female hormones can go up and down. This can cause ... hot flashes and vaginal dryness. Some women take hormone replacement therapy (HRT), also called menopausal hormone therapy, ...

  6. Growth hormone test

    ... page: // Growth hormone test To use the sharing features on this page, please enable JavaScript. The growth hormone test measures the amount of growth hormone in ...

  7. Influence of gonadotropin-releasing hormone agonist on the effect of chemotherapy upon ovarian cancer and the prevention of chemotherapy-induced ovarian damage: an experimental study with nu/nu athymic mice

    Qiong-yan LIN; Yi-feng WANG; Hui-nan WENG; Xiu-jie SHENG; Qing-ping JIANG; Zhi-ying YANG


    Background and objective:Gonadotropin-releasing hormone (GnRH) plays an important role in the regulation of ovarian function and ovarian cancer cell growth.In this study,we determined whether administration of the GnRH agonist (GnRHa),triporelin,prior to cisplatin treatment affects cisplatin and/or prevents cisplatin-induced ovarian damage.Methods:nu/nu mice were injected with ovarian cancer OVCAR-3 cells intraperitoneally.After two weeks,the mice were treated with saline (control),cisplatin,GnRHa,or cisplatin plus GnRHa for four weeks.At the end of the experimental protocol,blood,tumor,ovary,and uterine tissues were resected for hematoxylin and eosin (H&E) staining,immunohistochemical analyses of Ki67,nuclear factor-κB (NF-κB),and caspase-3,transmission electron microscopy of apoptosis,or enzyme-linked immunosorbent assay (ELISA) analyses of anti-Mullerian hormone (AMH).Results:Cisplatin treatment effectively inhibited tumor growth in mice treated with human ovarian cancer cells; however the treatment also induced considerable toxicity.Immunohistochemical analyses showed that Ki67 expression was reduced in cisplatin-treated mice compared to control (P<0.05),but there was no statistically significant differences between cisplatin-treated mice and cisplatin plus GnRHa-treated mice (P>0.05),while expressions of NF-κB and caspase-3 were reduced and induced,respectively,in cisplatin-treated mice and cisplatin plus GnRHa-treated mice.Apoptosis occurred in the GnRHa,cisplatin,and cisplatin plus GnRHa-treated mice,but not in control mice.Ovaries exposed to GnRHa in both GnRHa mice and cisplatin-treated mice (combination group) had significantly more primordial and growth follicles and serum levels of AMH than those in the control mice and cisplatin-treated mice (P<0.05).Conclusions:Administration of GnRHa to mice significantly decreased the extent of ovarian damage induced by cisplatin,but did not affect the anti-tumor activity of cisplatin.

  8. [Hormonal dysnatremia].

    Karaca, P; Desailloud, R


    Because of antidiuretic hormone (ADH) disorder on production or function we can observe dysnatremia. In the absence of production by posterior pituitary, central diabetes insipidus (DI) occurs with hypernatremia. There are hereditary autosomal dominant, autosomal recessive or X- linked forms. When ADH is secreted but there is an alteration on his receptor AVPR2, it is a nephrogenic diabetes insipidus in acquired or hereditary form. We can make difference on AVP levels and/or on desmopressine response which is negative in nephrogenic forms. Hyponatremia occurs when there is an excess of ADH production: it is a euvolemic hypoosmolar hyponatremia. The most frequent etiology is SIADH (syndrome of inappropriate secretion of ADH), a diagnostic of exclusion which is made after eliminating corticotropin deficiency and hypothyroidism. In case of brain injury the differential diagnosis of cerebral salt wasting (CSW) syndrome has to be discussed, because its treatment is perfusion of isotonic saline whereas in SIADH, the treatment consists in administration of hypertonic saline if hyponatremia is acute and/or severe. If not, fluid restriction demeclocycline or vaptans (antagonists of V2 receptors) can be used in some European countries. Four types of SIADH exist; 10 % of cases represent not SIADH but SIAD (syndrome of inappropriate antidiuresis) due to a constitutive activation of vasopressin receptor that produces water excess. c 2013 Published by Elsevier Masson SAS. PMID:24356291

  9. AMH and AFC as predictors of excessive response in controlled ovarian hyperstimulation : a meta-analysis

    Broer, S. L.; Dolleman, M.; Opmeer, B. C.; Fauser, B. C.; Mol, B. W.; Broekmans, F. J. M.


    background: Anti-Mullerian hormone (AMH) is a marker of ovarian reserve status and represents a good predictor of ovarian response to ovarian hyperstimulation. The aim of this study was to assess the accuracy of AMH and antral follicle count (AFC) as predictors of an excessive response in IVF/ICSI t

  10. Sertoli Cell Differentiation in Pubertal Boars

    Meishan boars experience puberty at a younger age than crossbred (BX) boars in association with earlier cessation of Sertoli cell proliferation and smaller post pubertal testicular size. The current study defined changes in expression, assessed by immunohistochemistry, of anti-Mullerian hormone (AMH...

  11. Relationship of antral follicle counts to fertility and ovarian AMH mRNA levels in beef cows

    In mammalian females, reproductive senescence is associated with decreased numbers of follicles in the ovary. Anti-Mullerian Hormone (AMH) of ovarian origin has been proposed to be a biomarker of the number of follicles in the ovary and of fertility in women, as well as to control activation of pri...

  12. Hormone Health Network

    ... reduce risk for other diseases The Hormone Health Network helps you and your health care provider have ... Copyright Endocrine Society. All rights reserved. Terms & Policies Network Partners The Hormone Health Network partners with other ...

  13. Hormones and Obesity

    ... Balance › Hormones and Obesity Fact Sheet Hormones and Obesity March, 2010 Download PDFs English Espanol Editors Caroline Apovian, MD Judith Korner, MD, PhD What is obesity? Obesity is a chronic (long-term) medical problem ...

  14. Menopause and Hormones

    ... Consumer Information by Audience For Women Menopause and Hormones: Common Questions Share Tweet Linkedin Pin it More ... reproduction and distribution. Learn More about Menopause and Hormones Menopause--Medicines to Help You Links to other ...

  15. Hormones and Hypertension

    Fact Sheet Hormones and Hypertension What is hypertension? Hypertension, or chronic (long-term) high blood pressure, is a main cause of ... tobacco, alcohol, and certain medications play a part. Hormones made in the kidneys and in blood vessels ...

  16. Hormone therapy in acne

    Chembolli Lakshmi


    Underlying hormone imbalances may render acne unresponsive to conventional therapy. Relevant investigations followed by initiation of hormonal therapy in combination with regular anti-acne therapy may be necessary if signs of hyperandrogenism are present. In addition to other factors, androgen-stimulated sebum production plays an important role in the pathophysiology of acne in women. Sebum production is also regulated by other hormones, including estrogens, growth hormone, insulin, insulin-l...

  17. Correlation between ultrasound parameters and hormone level in patients with polycystic ovary and polycystic ovary syndrome%多囊卵巢与多囊卵巢综合征的超声特征和激素水平相关性分析

    赵庆红; 石华; 杨菁; 胡佳琪; 张玉国; 陈茜; 刘艳


    将超声诊断为卵巢多囊样改变的104例患者按临床症状及内分泌检查分为多囊卵巢综合征(PCOS)组与单纯卵巢多囊样改变(PCO)组,同时纳入有正常排卵的妇女40人,做对照组,分别测定其卵巢面积(TA)、髓质面积(SA)及二者的比值(SA/TA),分析激素水平与SA/TA的相关性.PCO组的卵巢TA、SA及SA/TA、血清睾酮、苗勒氏管激素AMH及抑制素B均低于PCOS组,但高于对照组,差异具有统计学意义(P<0.05).PCOS患者血清睾酮水平与卵巢超声参数SA/TA有显著相关性(P<0.05).PCOS患者的AMH和抑制素B高于PCO组和对照组,说明AMH和抑制素B与卵巢多囊样改变的程度有关,可以用于鉴别PCOS和PCO.同时,SA/TA与PCOS血清睾酮升高有关,可以作为鉴别PCOS和PCO的内分泌指标.%A total of 104 patients with polycystic ovarian changes on ultrasound were divided into polycystic ovary syndrome (PCOS) and polycystic ovaries (PCO) groups according to their clinical symptoms and endocrine examinations.And 40 women with normal ovulation served as the controls.Ovarian volume (OV),ovarian stroma area (SA),ovarian total area (TA) and SA/TA ratio were measured by transvaginal uhrasonography.Their endocrine hormone levels were determined.Patients with PCOS showed significantly higher OV,SA,TA and SA/TA than those of PCO and control groups.SA/TA was positively correlated with testosterone.The median serum levels of anti-Mullerian hormone (AMH) and inhibit B in the PCO group were intermediate between those in control and PCOS groups.AMH and inhibin B of PCOS were higher than the PCO and control groups.It indicated that the levels of AMH and inhibit B were associated with the degree of polycystic ovaries and they could be used to differentiate PCOS and PCO.SA/TA is associated with the elevated serum testosterone of PCO.And SA/TA is an important ultrasonic parameter for diagnosing PCOS.

  18. Hormonal therapies in acne.

    Shaw, James C


    Hormones, in particular androgen hormones, are the main cause of acne in men, women, children and adults, in both normal states and endocrine disorders. Therefore, the use of hormonal therapies in acne is rational in concept and gratifying in practice. Although non-hormonal therapies enjoy wide usage and continue to be developed, there is a solid place for hormonal approaches in women with acne, especially adult women with persistent acne. This review covers the physiological basis for hormonal influence in acne, the treatments that are in use today and those that show promise for the future. The main treatments to be discussed are oral contraceptives androgen receptor blockers like spironolactone and flutamide, inhibitors of the enzyme 5 alpha-reductase and topical hormonal treatments. PMID:12083987

  19. Hormones and the pilosebaceous unit

    Chen, Wen-Chieh; Zouboulis, Christos C


    Hormones can exert their actions through endocrine, paracrine, juxtacrine, autocrine and intracrine pathways. The skin, especially the pilosebaceous unit, can be regarded as an endocrine organ meanwhile a target of hormones, because it synthesizes miscellaneous hormones and expresses diverse hormone receptors. Over the past decade, steroid hormones, phospholipid hormones, retinoids and nuclear receptor ligands as well as the so-called stress hormones have been demonstrated to play pivotal rol...

  20. Hormonal therapy for acne.

    George, Rosalyn; Clarke, Shari; Thiboutot, Diane


    Acne affects more than 40 million people, of which more than half are women older than 25 years of age. These women frequently fail traditional therapy and have high relapse rates even after isotretinoin. Recent advances in research have helped to delineate the important role hormones play in the pathogenesis of acne. Androgens such as dihydrotestosterone and testosterone, the adrenal precursor dehydroepiandrosterone sulfate, estrogens, growth hormone, and insulin-like growth factors may all contribute to the development of acne. Hormonal therapy remains an important part of the arsenal of acne treatments available to the clinician. Women dealing with acne, even those without increased serum androgens, may benefit from hormonal treatments. The mainstays of hormonal therapy include oral contraceptives and antiandrogens such as spironolactone, cyproterone acetate, or flutamide. In this article, we discuss the effects of hormones on the pathogenesis of acne, evaluation of women with suspected endocrine abnormalities, and the myriad of treatment options available. PMID:18786497

  1. Hormones and endometrial carcinogenesis.

    Kamal, Areege; Tempest, Nicola; Parkes, Christina; Alnafakh, Rafah; Makrydima, Sofia; Adishesh, Meera; Hapangama, Dharani K


    Endometrial cancer (EC) is the commonest gynaecological cancer in the Western World with an alarmingly increasing incidence related to longevity and obesity. Ovarian hormones regulate normal human endometrial cell proliferation, regeneration and function therefore are implicated in endometrial carcinogenesis directly or via influencing other hormones and metabolic pathways. Although the role of unopposed oestrogen in the pathogenesis of EC has received considerable attention, the emerging role of other hormones in this process, such as androgens and gonadotropin-releasing hormones (GnRH) is less well recognised. This review aims to consolidate the current knowledge of the involvement of the three main endogenous ovarian hormones (oestrogens, progesterone and androgens) as well as the other hormones in endometrial carcinogenesis, to identify important avenues for future research. PMID:26966933

  2. Adult growth hormone deficiency

    Vishal Gupta


    Adult growth hormone deficiency (AGHD) is being recognized increasingly and has been thought to be associated with premature mortality. Pituitary tumors are the commonest cause for AGHD. Growth hormone deficiency (GHD) has been associated with neuropsychiatric-cognitive, cardiovascular, neuromuscular, metabolic, and skeletal abnormalities. Most of these can be reversed with growth hormone therapy. The insulin tolerance test still remains the gold standard dynamic test to diagnose AGHD. Growth...

  3. Modelling plant hormone gradients.

    S. Moore; Zhang, X.; Liu, J; Lindsey, K.


    Cellular patterning in the Arabidopsis root is coordinated via a localised auxin concentration maximum in the root tip, requiring the regulated expression of specific genes. The activities of plant hormones such as auxin, ethylene and cytokinin depend on cellular context and exhibit either synergistic or antagonistic interactions. Due to the complexity and nonlinearity of spatiotemporal interactions between both hormones and gene expression in root development, modelling plant hormone gradien...

  4. Glucocorticoid and thyroid hormones transcriptionally regulate growth hormone gene expression.

    Evans, R M; Birnberg, N C; Rosenfeld, M G


    In order to define the molecular mechanisms by which glucocorticoids and thyroid hormone act to regulate growth hormone gene expression, the sites at which they exert their effects on growth hormone biosynthesis were examined in vivo and in a pituitary cell line. Glucocorticoids were shown to rapidly increase accumulation of growth hormone mRNA and nuclear RNA precursors. Glucocorticoids and thyroid hormone were shown to rapidly and independently increase growth hormone gene transcription. Th...

  5. Anti-Müllerian Hormone as a Biomarker of the Status of Ovarian Reserve after Chemotherapy in Rat Model%抗苗勒氏管激素作为预测化疗后卵巢储备功能状态的实验性研究

    吕向阳; 孙海梅; 路欣; 翁静; 梁元晶; 周德山


    目的:探讨化疗时抗苗勒氏管激素(anti-Mullerian hormone,AMH)作为预测大鼠卵巢储备功能状态的可行性.方法:雌性SD大鼠24只,随机分为3组:对照组(NS)、顺铂化疗低剂量组(4.5 mg/kg,CL)和高剂量组(6.0 mg/kg,CH),每组8只.免疫组织化学染色检测AMH在卵巢的表达,光学显微镜下计数AMH阳性卵泡百分比,ELISA检测血清AMH、FSH水平.结果:各组大鼠卵巢组织均可见AMH表达,主要位于初级卵泡、窦前卵泡及小窦状卵泡的颗粒细胞,给予顺铂12d后,AMH的表达强度减弱、范围缩小.CL组和CH组的AMH阳性卵泡百分比均较NS组明显降低(P

  6. Plant Hormone Binding Sites

    Napier, Richard


    • Aims Receptors for plant hormones are becoming identified with increasing rapidity, although a frustrating number remain unknown. There have also been many more hormone‐binding proteins described than receptors. This Botanical Briefing summarizes what has been discovered about hormone binding sites, their discovery and descriptions, and will not dwell on receptor functions or activities except where these are relevant to understand binding.

  7. Hormone therapy for prostate cancer

    ... this page: // Hormone therapy for prostate cancer To use the sharing ... helps slow the growth of prostate cancer. Male Hormones and Prostate Cancer Androgens are male sex hormones. ...

  8. Aging changes in hormone production

    ... this page: // Aging changes in hormone production To use the sharing ... that produce hormones are controlled by other hormones. Aging also changes this process. For example, an endocrine ...

  9. Hormones and female sexuality

    Bjelica Artur L.


    Full Text Available Introduction In contrast to animal species in which linear relationships exist between hormonal status and sexual behaviour sexuality in human population is not determined so simply by the level of sexual steroids. The article analyses female sexuality in the light of hormonal status. Administration of sexual steroids during pregnancy and sexual differentiation High doses of gestagens, especially those with high androgen activity, widely used against miscarriages may lead to tomboys, but without differences in sexual orientation. However, it has been observed that the frequency of bisexual and lesbian women is higher in women with congenital adrenogenital syndrome. Hormones sexual desire and sexuality during menstrual cycle It has been established that sexual desire, autoeroticism and sexual fantasies in women depend on androgen levels. There are a lot of reports claiming that sexual desire varies during the menstrual cycle. Hormonal contraception and sexuality Most patients using birth control pills present with decreased libido. But, there are reports that progestagens with antiandrogenic effect in contraceptive pills do not affect sexual desire. Hormonal changes in peri- and postmenopausal period and sexuality Decreased levels of estrogen and testosterone in older women are associated with decreased libido, sensitivity and erotic stimuli. Sexuality and hormone replacement therapy Hormonal therapy with estrogen is efficient in reference to genital atrophy, but not to sexual desire. Really increased libido is achieved using androgens. Also, therapy with dehydroepiandrosterone (DHEA and tibolone have positive effects on female libido. Conclusion Effect of sexual steroids on sexual sphere of women is very complex. The association between hormones and sexuality is multidimensional, as several hormones are important in regulation of sexual behaviour. Still, it should be pointed out that sexuality is in the domain of hormonal, emotional

  10. Ovarian function after removal of an entire ovary for cryopreservation of pieces of cortex prior to gonadotoxic treatment: a follow-up study

    Rosendahl, M.; Andersen, Claus Yding; Ernst, E.; Westergaard, L.G.; Rasmussen, P.E.; Loft, A.; Andersen, Anders Nyboe


    cancer (n = 31; 34%), Hodgkin's lymphoma (n = 23; 25%), bone marrow transplantation (BMT) (n = 19; 21%) and others (n = 19; 21%). Patients completed a questionnaire, and transvaginal ultrasonic antral follicle count and serum analysis for follicle stimulating hormone (FSH) and anti-Mullerian hormone (AMH...... with Hodgkin's lymphoma receiving more aggressive treatment than ABVD and in patients scheduled for BMT. The recommendation for breast cancer patient should be individualized. The cryopreservation process did not delay cancer treatment Udgivelsesdato: 2008/11...

  11. Ovarian Reserve Test L The Use in Daily Clinical Practice

    Abdullah, Nusratuddin


    To Evaluate the significance of conducting ovarian reserve testing decide when to order this testing in the clinical setting. and also to give individualized counseling to patients regarding the prognosis of infertility treatment based on their ovarian reserve tests, such as; basal antral follicle count, basal ovarian volume, ovarian stromal blood flow, ovarian biopsy, basal serum follicle stimulating hormone (FSH), basal serum estradiol, basal serum inhibin B, basal anti-Mullerian hormone s...

  12. Ovarian Reserve Test : The Use in Daily Clinical Practice

    Abdullah, Nusratuddin


    To Evaluate the significance of conducting ovarian reserve testing decide when to order this testing in the clinical setting. and also to give individualized counseling to patients regarding the prognosis of infertility treatment based on their ovarian reserve tests, such as; basal antral follicle count, basal ovarian volume, ovarian stromal blood flow, ovarian biopsy, basal serum follicle stimulating hormone (FSH), basal serum estradiol, basal serum inhibin B, basal anti-Mullerian hormone se...

  13. Growth hormone deficiency - children

    ... be done include: Insulin-like growth factor 1 (IGF-1) and insulin-like growth factor binding protein ... themselves the shot. Treatment with growth hormone is long-term, often lasting for several years. During this ...

  14. Growth hormone stimulation test

    The growth hormone (GH) stimulation test measures the ability of the body to produce GH. ... killing medicine (antiseptic). The first sample is drawn early in the morning. Medicine is given through the ...

  15. LH (Luteinizing Hormone) Test

    ... reason for the delayed puberty. Some of the causes for delayed puberty can include: Failure of the ovaries or testicles Hormone deficiency Turner syndrome Klinefelter syndrome Chronic infections Cancer Eating disorder (anorexia nervosa) ^ Back to top Is there anything ...

  16. Bioidentical Hormone Therapy

    Files, Julia A.; Ko, Marcia G.; Pruthi, Sandhya


    The change in hormonal milieu associated with perimenopause and menopause can lead to a variety of symptoms that can affect a woman's quality of life. Postmenopausal hormone therapy (HT) is an effective, well-tolerated treatment for these symptoms. However, combined HT consisting of conjugated equine estrogen and medroxyprogesterone acetate has been associated with an increased number of health risks when compared with conjugated equine estrogen alone or placebo. As a result, some women are t...

  17. Protein Hormones and Immunity‡

    Kelley, Keith W.; Weigent, Douglas A.; Kooijman, Ron


    A number of observations and discoveries over the past 20 years support the concept of important physiological interactions between the endocrine and immune systems. The best known pathway for transmission of information from the immune system to the neuroendocrine system is humoral in the form of cytokines, although neural transmission via the afferent vagus is well documented also. In the other direction, efferent signals from the nervous system to the immune system are conveyed by both the neuroendocrine and autonomic nervous systems. Communication is possible because the nervous and immune systems share a common biochemical language involving shared ligands and receptors, including neurotransmitters, neuropeptides, growth factors, neuroendocrine hormones and cytokines. This means that the brain functions as an immune-regulating organ participating in immune responses. A great deal of evidence has accumulated and confirmed that hormones secreted by the neuroendocrine system play an important role in communication and regulation of the cells of the immune system. Among protein hormones, this has been most clearly documented for prolactin (PRL), growth hormone (GH), and insulin-like growth factor-1 (IGF-I), but significant influences on immunity by thyroid stimulating hormone (TSH) have also been demonstrated. Here we review evidence obtained during the past 20 years to clearly demonstrate that neuroendocrine protein hormones influence immunity and that immune processes affect the neuroendocrine system. New findings highlight a previously undiscovered route of communication between the immune and endocrine systems that is now known to occur at the cellular level. This communication system is activated when inflammatory processes induced by proinflammatory cytokines antagonize the function of a variety of hormones, which then causes endocrine resistance in both the periphery and brain. Homeostasis during inflammation is achieved by a balance between cytokines and

  18. Gastrointestinal hormones regulating appetite

    Chaudhri, Owais; Small, Caroline; Bloom, Steve


    The role of gastrointestinal hormones in the regulation of appetite is reviewed. The gastrointestinal tract is the largest endocrine organ in the body. Gut hormones function to optimize the process of digestion and absorption of nutrients by the gut. In this capacity, their local effects on gastrointestinal motility and secretion have been well characterized. By altering the rate at which nutrients are delivered to compartments of the alimentary canal, the control of food intake arguably cons...

  19. Hormones and female sexuality

    Bjelica Artur L.; Kapamadžija Aleksandra; Maticki-Sekulić Milana


    Introduction In contrast to animal species in which linear relationships exist between hormonal status and sexual behaviour sexuality in human population is not determined so simply by the level of sexual steroids. The article analyses female sexuality in the light of hormonal status. Administration of sexual steroids during pregnancy and sexual differentiation High doses of gestagens, especially those with high androgen activity, widely used against miscarriages may lead to tomboys, but with...

  20. Body segments and growth hormone.

    Bundak, R; Hindmarsh, P. C.; Brook, C G


    The effects of human growth hormone treatment for five years on sitting height and subischial leg length of 35 prepubertal children with isolated growth hormone deficiency were investigated. Body segments reacted equally to treatment with human growth hormone; this is important when comparing the effect of growth hormone on the growth of children with skeletal dysplasias or after spinal irradiation.

  1. Kinetics of thyroid hormones

    Kinetics of thyroid hormones were outlined, and recent progress in metabolism of these hormones was also described. Recently, not only T4 and T3 but also rT3, 3,3'-T2, 3',5'-T2, and 3,5-T2 can be measured by RIA. To clarify metabolic pathways of these hormones, metabolic clearance rate and production rate of these hormones were calculated. As single-compartment analysis was insufficient to clarify disappearance curves of thyroid hormones in blood such as T3 and T2 of which metabolic speed was so fast, multi-compartment analysis or non-compartment analysis were also performed. Thyroid hormones seemed to be measured more precisely by constant infusion method. At the first step of T4 metabolism, T3 was formed by 5'-monodeiodination of T4, and rT3 was formed by 5-monodeiodination of T4. As metabolic pathways of T3 and rT3, conversion of them to 3,3'-T2 or to 3',5'-T2 and 3,5-T2 was supposed. This subject will be an interesting research theme in future. (Tsunoda, M.)

  2. Hormonal control of inflammatory responses

    Garcia-Leme, J.; Farsky, Sandra P


    Almost any stage of inflammatory and immunological responses is affected by hormone actions. This provides the basis for the suggestion that hormones act as modulators of the host reaction against trauma and infection. Specific hormone receptors are detected in the reactive structures in inflamed areas and binding of hormone molecules to such receptors results in the generation of signals that influence cell functions relevant for the development of inflammatory responses. Diversity of hormon...

  3. 围绝经期气郁质性激素与舌苔脱落细胞成熟指数、成熟价值的相关性%Correlation between Sex Hormones and Tongue Exfoliated Cells Maturation Index and Maturity Value in Perimenopause Women with Qi Stagnation

    李红; 陈以君; 任林; 李灿东


    目的 探讨围绝经期气郁质性激素及舌苔脱落细胞特点,以期为围绝经期气郁质的早期辨识提供帮助.方法 选取围绝经期平和质、气郁质妇女180例,根据月经情况分为绝经前期、绝经过渡期早期、绝经过渡期晚期,每种体质每期各30例,观察两组各期卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)、泌乳素(PRL)、抑制素B(InhB)、抗苗勒管激素(AMH)、舌苔脱落细胞成熟指效(MI)和成熟价值(MV),并分析各指标与气郁质的相关性.结果两组同期间FSH、LH、E2、PRL水平差异均具有统计学意义(P<0.05或P<0.01),气郁质组各指标的变化均较平和体质明显;两组FSH、LH、MV均随着围绝经期的进程而明显升高(P<0.01),E2、PRL、AMH、InhB则均明显下降(P<0.01). 结论 围绝经期气郁质妇女围绝经期进程较平和质快,且性激素、MI、MV改变与气郁质有明显相关性,可作为围绝经期气郁质早期判定的参考指标.%Objective To research the characteristics of sex hormones and exfoliated cell of tongue fur in qi stagnation constitution during the perimenopause and provide help for the early identification. Methods Totally 180 women of mild constitution and qi stagnation constitution were selected and divided into premenopause. early menopausal transition and late menopaus-al transition according to the menstruation. Thirty cases were selected in each constitution group and during each period. The correlation between qi stagnation constitution and follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), prolactin (PRL), inhibin B (InhB), anti-Mullerian hormone (AMH) and the maturation index (MI) and maturity value (MV) of tongue off cell were analyzed. Results The differences of FSH, LH, E2 and PRL were statistically significant between two constitution groups during each period (P<0.01), especially in qi stagnation constitution group. FSH, LH and MV significantly

  4. Headache And Hormones

    Shukla Rakesh


    Full Text Available There are many reasons to suggest a link between headache and hormones. Migraine is three times common in women as compared to men after puberty, cyclic as well as non-cyclic fluctuations in sex hormone levels during the entire reproductive life span of a women are associated with changes in frequency or severity of migraine attack, abnormalities in the hypothalamus and pineal gland have been observed in cluster headache, oestrogens are useful in the treatment of menstrual migraine and the use of melatonin has been reported in various types of primary headaches. Headache associated with various endocrinological disorders may help us in a better understanding of the nociceptive mechanisms involved in headache disorders. Prospective studies using headache diaries to record the attacks of headache and menstrual cycle have clarified some of the myths associated with menstrual migraine. Although no change in the absolute levels of sex hormones have been reported, oestrogen withdrawal is the most likely trigger of the attacks. Prostaglandins, melatonin, opioid and serotonergic mechanisms may also have a role in the pathogenesis of menstrual migraine. Guidelines have been published by the IHS recently regarding the use of oral contraceptives by women with migraine and the risk of ischaemic strokes in migraineurs on hormone replacement therapy. The present review includes menstrual migraine, pregnancy and migraine, oral contraceptives and migraine, menopause and migraine as well as the hormonal changes in chronic migraine.

  5. Growth Hormone Deficiency in Children

    ... which the body does not make enough growth hormone (GH). GH is made by the pituitary gland, a ... blood test checks levels of IGF-1, a hormone that reflects GH levels. • GH stimulation test. The child is given ...

  6. Aging changes in hormone production

    ... that produce hormones are controlled by other hormones. Aging also changes this process. For example, an endocrine ... produce the same amount at a slower rate. AGING CHANGES The hypothalamus is located in the brain. ...

  7. Luteinizing hormone (LH) blood test

    ICSH - blood test; Luteinizing hormone - blood test; Interstitial cell stimulating hormone - blood test ... to temporarily stop medicines that may affect the test results. Be sure to tell your provider about ...

  8. SHBG (Sex Hormone Binding Globulin)

    ... as: Testosterone-estrogen Binding Globulin; TeBG Formal name: Sex Hormone Binding Globulin Related tests: Testosterone , Free Testosterone, ... I should know? How is it used? The sex hormone binding globulin (SHBG) test may be used ...

  9. Hormonal contraception and venous thromboembolism

    Lidegaard, Øjvind; Milsom, Ian; Geirsson, Reynir Tomas;


    New studies about the influence of hormonal contraception on the risk of venous thromboembolism (VTE) have been published.......New studies about the influence of hormonal contraception on the risk of venous thromboembolism (VTE) have been published....

  10. Gastrointestinal hormones and their targets

    Rehfeld, Jens F.


    Gastrointestinal hormones are peptides released from endocrine cells and neurons in the digestive tract. More than 30 hormone genes are currently known to be expressed in the gastrointestinal tract, which makes the gut the largest hormone producing organ in the body. Modern biology makes it...... feasible to conceive the hormones under five headings: The structural homology groups a majority of the hormones into nine families, each of which is assumed to originate from one ancestral gene. The individual hormone gene often has multiple phenotypes due to alternative splicing, tandem organization, or...... differentiated maturation of the prohormone. By a combination of these mechanisms, more than 100 different hormonally active peptides are released from the gut. Gut hormone genes are also widely expressed in cells outside the gut, some only in extraintestinal endocrine cells and neurons but others also in other...

  11. Radioimmunoassay of steroid hormone

    Low acid pepsin treated gamma-globulin was applied to ammonium sulfate salting out method, which was a method to separate bound fraction from free one in radioimmunoassay of steroid hormone, and the effect of the separation and the standard curve were examined. Pepsin treated gamma-globulin was prepared in pH 1.5 to 5.5 and then the pepsin was completely removed. It had an effect to accelerate the precipitation in radioimmunoassay of steroid hormone labelled with 3H. The effect of pepsin treated gamma-globulin to adhere free steroid hormone and to slat out bound one was compared with that of human gamma-globulin. Pepsin treated gamma-globulin, which was water soluble, could easier reach its optimal concentration, and the separation effect was better than human gamma-globulin. The standard curve of it was steeper, particularly in a small dose, and the reproducibility was also better. It could be applied not only to aldosterone and DOC, but also to the steroid hormones, such as progesterone and DHEA, and it seemed suitable for routine measurement method. (Kanao, N.)

  12. Hormone Therapy for Breast Cancer in Men

    ... Topic Targeted therapy for breast cancer in men Hormone therapy for breast cancer in men Hormone therapy ... fatigue, and pain at the injection site. Luteinizing hormone-releasing hormone (LHRH) analogs and anti-androgens LHRH ...

  13. Growth Hormone: Use and Abuse

    ... is huma n gr owth hormone? Human growth hormone (GH) is a substance that controls your body’s growth. ... prescribed for the FDA-approved conditions. In children, GH is used to treat • Growth hormone deficiency • Conditions that cause short stature (being shorter ...


    Siddalingeshwar V


    Full Text Available : A 3-year-old boy presenting with right inguinal hernia was found to have uterus, fallopian tube and testes in a position analogous to ovaries in hernial sac, warranting a diagnosis of persistent Mullerian duct syndrome (PMDS. PMDS is an extremely rare form of internal male pseudo-hermaphroditism in which female internal sex organs, including the uterus, cervix and proximal vagina, persist in a 46XY male with normal external genitalia. The condition results from a congenital insensitivity to anti-Mullerian hormone, or lack of anti-Mullerian hormone, leading to persistence of the female internal sex organs in a male. Clinically, this condition is associated with cryptochoridism. Controversy persists regarding the appropriate treatment of PMDS, since resection of the remnant structures is associated with potential morbidity, but retention risks development of occasional malignancies. We review the literature and discuss various aspects of pathophysiology, diagnosis, and management of PMDS.

  15. Vitamin D: potential clinical decisions in doctor practice

    Комісаренко, Ю. І.; Бобрик, М. І.; Сідорова, І. В.; БУРКА О.А.


    It is proved that vitamin D plays a role in the prevention and treatment of many diseases such as immunodeficiency status, anemia, diabetes melitus, various pathological conditions of the liver, gastrointestinal tract, cardiovascular system, tuberculosis, tumors of breast, bowel, etc. Also it was found that vitamin D modulates reproductive processes in women particularly affects steroidogenesis of estradiol and progesterone, and anti-Mullerian hormone in healthy women.Therefore, at examining ...

  16. Hormonal control of implantation.

    Sandra, Olivier


    In mammals, implantation represents a key step of pregnancy and its progression conditions not only the success of pregnancy but health of the offspring. Implantation requires a complex and specific uterine tissue, the endometrium, whose biological functions are tightly regulated by numerous signals, including steroids and polypeptide hormones. Endometrial tissue is endowed with dynamic properties that associate its ability to control the developmental trajectory of the embryo (driver property) and its ability to react to embryos displaying distinct capacities to develop to term (sensor property). Since dynamical properties of the endometrium can be affected by pre- and post-conceptional environment, determining how maternal hormonal signals and their biological actions are affected by environmental factors (e.g. nutrition, stress, infections) is mandatory to reduce or even to prevent their detrimental effects on endometrial physiology in order to preserve the optimal functionality of this tissue. PMID:27172870

  17. Hormones in pregnancy

    Pratap Kumar


    Full Text Available The endocrinology of human pregnancy involves endocrine and metabolic changes that result from physiological alterations at the boundary between mother and fetus. Progesterone and oestrogen have a great role along with other hormones. The controversies of use of progestogen and others are discussed in this chapter. Progesterone has been shown to stimulate the secretion of Th2 and reduces the secretion of Th1 cytokines which maintains pregnancy. Supportive care in early pregnancy is associated with a significant beneficial effect on pregnancy outcome. Prophylactic hormonal supplementation can be recommended for all assisted reproduction techniques cycles. Preterm labor can be prevented by the use of progestogen. The route of administration plays an important role in the drug′s safety and efficacy profile in different trimesters of pregnancy. Thyroid disorders have a great impact on pregnancy outcome and needs to be monitored and treated accordingly. Method of locating review: Pubmed, scopus

  18. The wound hormone jasmonate

    Koo, Abraham J.K.; Howe, Gregg A.


    Plant tissues are highly vulnerable to injury by herbivores, pathogens, mechanical stress, and other environmental insults. Optimal plant fitness in the face of these threats relies on complex signal transduction networks that link damage-associated signals to appropriate changes in metabolism, growth, and development. Many of these wound-induced adaptive responses are triggered by de novo synthesis of the plant hormone jasmonate (JA). Recent studies provide evidence that JA mediates systemic...

  19. Growth Hormone Promotes Lymphangiogenesis

    Banziger-Tobler, Nadja Erika; Halin, Cornelia; Kajiya, Kentaro; Detmar, Michael


    The lymphatic system plays an important role in inflammation and cancer progression, although the molecular mechanisms involved are poorly understood. As determined using comparative transcriptional profiling studies of cultured lymphatic endothelial cells versus blood vascular endothelial cells, growth hormone receptor was expressed at much higher levels in lymphatic endothelial cells than in blood vascular endothelial cells. These findings were confirmed by quantitative real-time reverse tr...

  20. Study of anti - Mūllerian hormone in prediction of poor ovarian response%抗苗勒管激素对卵巢低反应预测价值的研究

    朱英哲; 彭沙; 王青欣; 杨晓葵


    Objective: To evaluate anti - Mullerian hormone (AMH) in prediction of poor ovarian response to controlled ovarian hyperstimulation (COH) in assisted reproductive technology (ART). Methods; The level of bAMH protein secretion in serum was examined by ELISA in 181 cases of infertile woman with first cycle of in vitro fertilization (IVF) / intracytoplasmic sperm injection (IC-SI). The patients were classified into 2 groups including poor response (n =20, the number of oocytes retrieved ≤5 and normal response (n = 161, the number of oocytes retrieved > 5) according to ovarian response to COH. The relationship among bAMH level, age and the number of oocytes retrieved was analyzed by software engineering SPSS11.5. Results; ① A positive correlation was found between serum bAMH and the number of oocytes retrieved (r=0.709, P5) (P <0.05). ③ ROC curve analysis revealed that area under curve (AUC) was 0. 949 for bAMH. The sensitivity and specificity were 86. 3% and 90.0% , respectively when the cutoff value of bAMH was no higher than 3.65ng/ml. Conclusion; These results indicate that serum AMH represents the well endocrine marker to assess the poor ovarian response on COH in ART.%目的 探讨抗苗勒氏管激素(AMH)在辅助生殖技术(ART)中对控制性超排卵(COH)卵巢低反应的预测价值.方法 利用ELISA法对181例首次接受IVF/ICSI - ET治疗的不孕症患者基础血清AMH蛋白水平(bAMH)进行检测.根据患者COH卵巢反应将患者分为2组:卵巢低反应组20例(获卵数≤5个)和卵巢反应良好组161例(获卵数>5个).比较bAMH与年龄、获卵数、基础FSH等的相关性.结果 ①181例不孕症患者bAMH水平与获卵数呈显著正相关(r =0.709,P<0.05);②卵巢低反应组(获卵数≤5个)与反应良好组(获卵数>5个)的bAMH水平比较,差异有统计学意义(P<0.05);③受试者工作特征(ROC)曲线分析显示,bAMH水平的曲线下面积(AUC)为0.949.当bAMH截断值≤3.65ng

  1. Quo vadis plant hormone analysis?

    Tarkowská, D. (Danuše); Novák, O. (Ondřej); Floková, K. (Kristýna); Tarkowski, P.; Turečková, V. (Veronika); Grúz, J. (Jiří); Rolčík, J. (Jakub); Strnad, M.


    Plant hormones act as chemical messengers in the regulation of myriads of physiological processes that occur in plants. To date, nine groups of plant hormones have been identified and more will probably be discovered. Furthermore, members of each group may participate in the regulation of physiological responses in planta both alone and in concert with members of either the same group or other groups. The ideal way to study biochemical processes involving these signalling molecules is 'hormon...

  2. Mammalian sex hormones in plants

    Andrzej Skoczowski; Anna Janeczko


    The occurrence of mammalian sex hormones and their physiological role in plants is reviewed. These hormones, such as 17β-estradiol, androsterone, testosterone or progesterone, were present in 60-80% of the plant species investigated. Enzymes responsible for their biosynthesis and conversion were also found in plants. Treatment of the plants with sex hormones or their precursors influenced plant development: cell divisions, root and shoot growth, embryo growth, flowering, pollen tube ...

  3. Hormones and Borderline Personality Features

    Evardone, Milagros; Alexander, Gerianne M.; Morey, Leslie C.


    Borderline personality is diagnosed in clinical settings three times more often in women than in men, and symptom severity in women appears sensitive to circulating sex steroid levels. In non-human mammals, prenatal hormones contribute to the development of sex-linked behavior and their responsiveness to postnatal hormones. Therefore, this study examined the hypothesis that prenatal hormones may influence the development of borderline personality traits by measuring a marker of perinatal andr...

  4. Growth hormone and aging

    Bartke, Andrzej; Brown-Borg, Holly; Kinney, Beth; Mattison, Julie; Wright, Chris; Hauck, Steven; Coschigano, Karen; Kopchick, John


    The potential usefulness of growth hormone (GH) as an anti-aging therapy is of considerable current interest. Secretion of GH normally declines during aging and administration of GH can reverse age-related changes in body composition. However, mutant dwarf mice with congenital GH deficiency and GH resistant GH-R-KO mice live much longer than their normal siblings, while a pathological elevation of GH levels reduces life expectancy in both mice and men. We propose that the actions of GH on gro...

  5. 抗苗勒激素对体外受精-胚胎移植结局的预测作用%Anti-müllerian Hormone Has an Predictive Effect on the Outcome of In Vitro Fertilization-Embryo Transfer

    程萍; 张丽萍; 刘永珍


    Objective To study the relationships between anti - miillerian hormone in serum and follicular fluid and embryonic quality and successful pregnancy. Methods Enzyme-linked immunosorbent assay (ELJSA) was used to detect anti -mullerian hormone(AMH) levels in serum on the day of oocyte pick-up(OPU),embryo transfer(ET) ,ET + 3 and in follicular Quid on the day of OPU in 48 patients(48 cycles) undergoing in vitro fertilization-embryo transfer(IVF-ET). Results (l)The AMH concentrations in follicular fluid of patients whose high-quality embryonic ratio is no less than 0.5 are significantly higher than those whose high-quality embryonic ratio is less than 0.5 (P 0.05, respectively). (3) However,the AMH concentration in follicular fluid of pregnant group are markedly higher than non-pregnant group( P < 0. 05). Conclusion The AMH levels of every stage after OPU in serum can not predict the outcome of IVF-ET. The AMH concentration in follicular fluid is a factor that could be used to predict the outcome of IVF-ET. It may lead to the change of embryonic quality by altering follicular microenvironment locally and then affecting the outcome of IVF-ET.%目的:研究行体外受精-胚胎移植(IVF-ET)的患者血清及卵泡液中抗苗勒激素(AMH)与胚胎质量、妊娠结局之间的关系.方法:酶联免疫吸附实验(ELISA)检测48例行IVF-ET患者(48个周期)取卵日、胚胎移植日和胚胎移植后3d血清中AMH水平及取卵日卵泡液中AMH水平,了解其与胚胎质量、妊娠结局之间的关系.结果:(1)高质量胚胎比≥0.5组卵泡液AMH水平较高质量胚胎比<0.5组显著升高(P<0.05);(2)在妊娠组与非妊娠组之间,取卵日、胚胎移植日和胚胎移植后3d血清中AMH水平无差异(P>0.05);(3)妊娠组卵泡液AMH水平明显高于非妊娠组(P<0.05).结论:取卵后各个时期的血清AMH水平不能预测IVF-ET妊娠结局,取卵日卵泡液中AMH水平可以作为IVF-ET妊娠结局的预测因子.卵泡液

  6. Alternatives of menopausal hormone therapy

    Kutlešić Ranko M.; Popović Jasmina; Stefanović Milan; Vukomanović Predrag; Lukić Bojan; Lilić Goran


    Introduction. It has been generally accepted that the benefits of menopausal hormone therapy outweigh the risks, but there are still some concerns about the administration of menopausal hormone therapy, which has introduced alternative treatments. Pharmacological Alternatives. Central alpha-2 agonist clonidine is only marginally more effective than placebo, and significantly less effective than estrogen. Antiepileptic drug gabapentin reduces hot flashes; ho...

  7. Hormonal control of inflammatory responses

    J. Garcia-Leme


    Full Text Available Almost any stage of inflammatory and immunological responses is affected by hormone actions. This provides the basis for the suggestion that hormones act as modulators of the host reaction against trauma and infection. Specific hormone receptors are detected in the reactive structures in inflamed areas and binding of hormone molecules to such receptors results in the generation of signals that influence cell functions relevant for the development of inflammatory responses. Diversity of hormonal functions accounts for recognized pro- and anti-inflammatory effects exerted by these substances. Most hormone systems are capable of influencing inflammatory events. Insulin and glucocorticoids, however, exert direct regulatory effects at concentrations usually found in plasma. Insulin is endowed with facilitatory actions on vascular reactivity to inflammatory mediators and inflammatory cell functions. Increased concentrations of circulating glucocorticoids at the early stages of inflammation results in downregulation of inflammatory responses. Oestrogens markedly reduce the response to injury in a variety of experimental models. Glucagon and thyroid hormones exert indirect anti-inflammatory effects mediated by the activity of the adrenal cortex. Accordingly, inflammation is not only merely a local response, but a hormone-controlled process.

  8. Recent advances in hormonal contraception

    Li, HW Raymond; Richard A. Anderson


    This report reviews some of the new studies regarding new hormonal contraceptive formulations (e.g., Yaz, Qlaira®, extended-cycle or continuous combined contraceptives, subcutaneous depot medroxyprogesterone acetate, and ulipristal acetate as an emergency contraceptive). Recent data on the relationship between hormonal contraceptive use and bone health are also reviewed. © 2010 Medicine Reports Ltd.

  9. Hormonal contraception, thrombosis and age

    Lidegaard, Øjvind


    INTRODUCTION: This paper reviews the risk of thrombosis with use of different types of hormonal contraception in women of different ages. AREAS COVERED: Combined hormonal contraceptives with desogestrel, gestodene, drospirenone or cyproterone acetate (high-risk products) confer a sixfold increased...

  10. [Do hormones determine our fate?].

    Vermeulen, A


    The hormonal system is a communication system between cells and organs. Hence it is not surprising that it influences almost all physiological functions and, at least partially, our behaviour and fate. The sexual phenotype is determined by the sex hormones. Normally, the phenotype is in accordance with gonadal and genetic sex, but occasionally, as a consequence of enzymatic defects in the biosynthesis of sex hormones or of androgen resistance, gonadal and genetic sex are in discordance with the phenotype, the latter determining generally the civil sex and the sex of rearing. Whereas the gender role is generally determined by the sex of rearing and the phenotype, itself under hormonal influence, homo- and transsexuality constitute notorious exceptions to this rule. Although several authors consider homo- and transsexuality to be the consequence of an impairment in androgenic impregnation in the perinatal period, there are at present no convincing arguments for an hormonal origin for either homo- or transsexuality, although such a possibility can't be excluded either. Besides their role in psychosexual behaviour, sex hormones play also a role in our life expectancy. Indeed, although maximal life expectancy of man is genetically determined, a major determinant of individual life expectancy is cardiovascular pathology. The latter is partly responsible for the difference in life expectancy between men and women, cardiovascular mortality increasing rapidly at menopause and being halved by oestrogen replacement therapy. Also atherogenesis as such is, to a large extend, under hormonal control. Indeed insulin resistance and hyperinsulinism, which develop as a corollary of the aging process, is an important cause of atherosclerosis as well as of hypertension. Other hormones also play an important role in our behaviour. We can mention here the role of the thyroid hormones in the physical and mental development of children as well as in the regression of the intellectual

  11. Estrogen and Progestin (Hormone Replacement Therapy)

    Combinations of estrogen and progestin are used to treat certain symptoms of menopause. Estrogen and progestin are two female sex hormones. Hormone replacement therapy works by replacing estrogen hormone that is no longer being made by ...

  12. Genetics Home Reference: combined pituitary hormone deficiency

    ... Genetics Home Health Conditions combined pituitary hormone deficiency combined pituitary hormone deficiency Enable Javascript to view the ... boxes. Print All Open All Close All Description Combined pituitary hormone deficiency is a condition that causes ...

  13. Leptin: a multifunctional hormone


    Leptin is the protein product encoded by the obese (ob)gene. It is a circulating hormone produced primarily by the adipose tissue. ob/ob mice with mutations of the gene encoding leptin become morbidly obese, infertile, hyperphagic, hypothermic,and diabetic. Since the cloning of leptin in 1994, our knowledge in body weight regulation and the role played by leptin has increased substantially. We now know that leptin signals through its receptor, OB-R, which is a member of the cytokine receptor superfamily. Leptin serves as an adiposity signal to inform the brain the adipose tissue mass in a negative feedback loop regulating food intake and energy expenditure. Leptin also plays important roles in angiogenesis, immune function, fertility, and bone formation. Humans with mutations in the gene encoding leptin are also morbidly obese and respond to leptin treatment,demonstrating that enhancing or inhibiting leptin's activities in vivo may have potential therapeutic benefits.

  14. Testes and brain gene expression in precocious male and adult maturing Atlantic salmon (Salmo salar

    Houeix Benoit


    Full Text Available Abstract Background The male Atlantic salmon generally matures in fresh water upon returning after one or several years at sea. Some fast-growing male parr develop an alternative life strategy where they sexually mature before migrating to the oceans. These so called 'precocious' parr or 'sneakers' can successfully fertilise adult female eggs and so perpetuate their line. We have used a custom-built cDNA microarray to investigate gene expression changes occurring in the salmon gonad and brain associated with precocious maturation. The microarray has been populated with genes selected specifically for involvement in sexual maturation (precocious and adult and in the parr-smolt transformation. Results Immature and mature parr collected from a hatchery-reared stock in January were significantly different in weight, length and condition factor. Changes in brain expression were small - never more than 2-fold on the microarray, and down-regulation of genes was much more pronounced than up-regulation. Significantly changing genes included isotocin, vasotocin, cathepsin D, anamorsin and apolipoprotein E. Much greater changes in expression were seen in the testes. Among those genes in the testis with the most significant changes in expression were anti-Mullerian hormone, collagen 1A, and zinc finger protein (Zic1, which were down-regulated in precocity and apolipoproteins E and C-1, lipoprotein lipase and anti-leukoproteinase precursor which were up-regulated in precocity. Expression changes of several genes were confirmed in individual fish by quantitative PCR and several genes (anti-Mullerian hormone, collagen 1A, beta-globin and guanine nucleotide binding protein (G protein beta polypeptide 2-like 1 (GNB2L1 were also examined in adult maturing testes. Down-regulation of anti-Mullerian hormone was judged to be greater than 160-fold for precocious males and greater than 230-fold for November adult testes in comparison to July testes by this method. For

  15. Genetics Home Reference: isolated growth hormone deficiency

    ... deficiency dwarfism, pituitary growth hormone deficiency dwarfism isolated GH deficiency isolated HGH deficiency isolated human growth hormone deficiency isolated somatotropin deficiency isolated somatotropin deficiency disorder ...

  16. Genotoxic potential of nonsteroidal hormones

    Topalović Dijana


    Full Text Available Hormones are cellular products involved in the regulation of a large number of processes in living systems, and which by their actions affect the growth, function and metabolism of cells. Considering that hormones are compounds normally present in the organism, it is important to determine if they can, under certain circumstances, lead to genetic changes in the hereditary material. Numerous experimental studies in vitro and in vivo in different systems, from bacteria to mammals, dealt with the mutagenic and genotoxic effects of hormones. This work presents an overview of the research on genotoxic effects of non­steroidal hormones, although possible changes of genetic material under their influence have not still been known enough, and moreover, investigations on their genotoxic influence have given conflicting results. The study results show that mechanisms of genotoxic effect of nonsteroidal hormones are manifested through the increase of oxidative stress by arising reactive oxygen species. A common mechanism of ROS occurence in thyroid hormones and catecholamines is through metabolic oxidation of their phenolic groups. Manifestation of insulin genotoxic effect is based on production of ROS by activation of NADPH isophorms, while testing oxytocin showed absence of genotoxic effect. Considering that the investigations on genotoxicity of nonsteroidal hormones demonstrated both positive and negative results, the explanation of this discordance involve limitations of test systems themselves, different cell types or biological species used in the experiments, different level of reactivity in vitro and in vivo, as well as possible variations in a tissue-specific expression. Integrated, the provided data contribute to better understanding of genotoxic effect of nonsteroidal hormones and point out to the role and mode of action of these hormones in the process of occurring of effects caused by oxidative stress. [Projekat Ministarstva nauke Republike

  17. Micro-electrolytic iodination of polypeptide hormones

    This report describes a constant voltage microelectrolytic 125I-labelling procedure and applies it to the iodination of 5-50 μg quantities of polypeptide hormones (synthetic salmon calcitonin, porcine glucagon, dog growth hormone, bovine growth hormone, bovine lutenizing hormone, bovine parathyroid hormone and bovine thyroid stimulating hormone). The electrolytic technique avoids exposure of the hormones to oxidizing agents which damage hormones and alter their biological and immunological activity. The labeled hormones showed no apparent damage by chromatoelectrophoresis or polyacrylamide gel filtration and all of the labelled hormones tested were either biologically or immunologically active. Finally, this simple, mild, and rapid micro-electrolytic iodination technique is highly reproducible, and rapid micro-electrolytic iodination technique is highly reproducible, yields a high degree of iodination and allows for the preparation of either high or low specific activity labeled hormone molecules. (author)

  18. Sex Hormones and Ischemic Stroke

    Holmegard, Haya N; Nordestgaard, Børge G; Jensen, Gorm B;


    = 4615) and women (n = 4724) with measurements of endogenous sex hormones during the 1981-1983 examination of the Copenhagen City Heart Study, Denmark, were followed for up to 29 years for incident IS, with no loss to follow-up. Mediation analyses assessed whether risk of IS was mediated through......CONTEXT AND OBJECTIVE: Whether endogenous sex hormones are associated with ischemic stroke (IS) is unclear. We tested the hypothesis that extreme concentrations of endogenous sex hormones are associated with risk of IS in the general population. DESIGN, SETTING, AND PARTICIPANTS: Adult men (n...

  19. Hormone Therapy for Prostate Cancer

    ... agonists , which are sometimes called LHRH analogs, are synthetic proteins that are structurally similar to LHRH and ... gland to stop producing luteinizing hormone, which prevents testosterone from being produced. Treatment with an LHRH agonist ...

  20. Thyroid Hormone and Vascular Remodeling.

    Ichiki, Toshihiro


    Both hyperthyroidism and hypothyroidism affect the cardiovascular system. Hypothyroidism is known to be associated with enhanced atherosclerosis and ischemic heart diseases. The accelerated atherosclerosis in the hypothyroid state has been traditionally ascribed to atherogenic lipid profile, diastolic hypertension, and impaired endothelial function. However, recent studies indicate that thyroid hormone has direct anti-atherosclerotic effects, such as production of nitric oxide and suppression of smooth muscle cell proliferation. These data suggest that thyroid hormone inhibits atherogenesis through direct effects on the vasculature as well as modification of risk factors for atherosclerosis. This review summarizes the basic and clinical studies on the role of thyroid hormone in vascular remodeling. The possible application of thyroid hormone mimetics to the therapy of hypercholesterolemia and atherosclerosis is also discussed. PMID:26558400

  1. Revisiting Thyroid Hormones in Schizophrenia

    Nadine Correia Santos


    Full Text Available Thyroid hormones are crucial during development and in the adult brain. Of interest, fluctuations in the levels of thyroid hormones at various times during development and throughout life can impact on psychiatric disease manifestation and response to treatment. Here we review research on thyroid function assessment in schizophrenia, relating interrelations between the pituitary-thyroid axis and major neurosignaling systems involved in schizophrenia’s pathophysiology. These include the serotonergic, dopaminergic, glutamatergic, and GABAergic networks, as well as myelination and inflammatory processes. The available evidence supports that thyroid hormones deregulation is a common feature in schizophrenia and that the implications of thyroid hormones homeostasis in the fine-tuning of crucial brain networks warrants further research.

  2. Measurement of the incretin hormones

    Kuhre, Rune Ehrenreich; Wewer Albrechtsen, Nicolai Jacob; Hartmann, Bolette;


    The two incretin hormones, glucagon-like peptide 1 (GLP-1) and glucose-dependent insulinotropic peptide (GIP), are secreted from the gastrointestinal tract in response to meals and contribute to the regulation of glucose homeostasis by increasing insulin secretion. Assessment of plasma concentrat......The two incretin hormones, glucagon-like peptide 1 (GLP-1) and glucose-dependent insulinotropic peptide (GIP), are secreted from the gastrointestinal tract in response to meals and contribute to the regulation of glucose homeostasis by increasing insulin secretion. Assessment of plasma...... concentrations of GLP-1 and GIP is often an important endpoint in both clinical and preclinical studies and, therefore, accurate measurement of these hormones is important. Here, we provide an overview of current approaches for the measurement of the incretin hormones, with particular focus on immunological...

  3. Plant hormone receptors: new perceptions

    Spartz, Angela K.; William M Gray


    Plant growth and development require the integration of a variety of environmental and endogenous signals that, together with the intrinsic genetic program, determine plant form. Central to this process are several growth regulators known as plant hormones or phytohormones. Despite decades of study, only recently have receptors for several of these hormones been identified, revealing novel mechanisms for perceiving chemical signals and providing plant biologists with a much clearer picture of...

  4. History of growth hormone therapy

    Vageesh S Ayyar


    Although the importance of the pituitary gland for growth was recognized in late 19 th century, Growth hormone (GH) therapy was made available for severely GH-deficient children and adolescents only in late 1950s. Use of GH for other conditions was limited because of the limited supply of human pituitary-derived hormone. With unlimited availability of recombinant human GH (rhGH), the scenario of GH treatment has been changed enormously. Currently there is ever increasing list of indications o...

  5. Stress hormones and physical activity

    Editorial Office


    Hormone secretion during physical activity of specific duration and intensity is part of the stress response. In a study to investigate the secretion of ß-endorphin, leucine enkephalin and other recognised stress hormones during physical exercise, blood samples were taken from fourteen (14) healthy, male athletes who competed in a 21 km roadrace. Blood samples were collected before and after completion of the race. This study shows that ß-endorphin/ß-lipotropin, leucine enkephalin, prolact...

  6. Fatigue and cognition - hormonal perspectives

    Möller, Marika


    Fatigue is a common complaint and considered a very challenging symptom to cope with in many different medical diseases. The assessment of fatigue is bound up with the problems of both conceptualization and definition. In addition, few studies have investigated suitable neuropsychological tests to examine fatigue and its consequences. This thesis evaluates whether neuropsychological tests can elicit cognitive fatigue. It also investigates whether specific hormones and hormon...

  7. Revisiting Thyroid Hormones in Schizophrenia

    Nadine Correia Santos; Patrício Costa; Dina Ruano; António Macedo; Maria João Soares; José Valente; Ana Telma Pereira; Maria Helena Azevedo; Joana Almeida Palha


    Thyroid hormones are crucial during development and in the adult brain. Of interest, fluctuations in the levels of thyroid hormones at various times during development and throughout life can impact on psychiatric disease manifestation and response to treatment. Here we review research on thyroid function assessment in schizophrenia, relating interrelations between the pituitary-thyroid axis and major neurosignaling systems involved in schizophrenia’s pathophysiology. These include the ser...

  8. Short Stature and Growth Hormone Deficiency

    Matemi, Sezer


    This paper summarizes the importance of measurements of height and weight in normal children and emphasizes the role of growth increments for the diagnosis of short stature Causes of short stature methods for diagnosis of GH hormone deficiency actions of growth hormone treatment of growth hormone deficiency and doses for biosynthetic GH treatment are described Key words: Short Stature Growth Hormone

  9. Hormone therapy and ovarian borderline tumors

    Mørch, Lina Steinrud; Løkkegaard, Ellen; Andreasen, Anne Helms;


    Little is known about the influence of postmenopausal hormone therapy on the risk of ovarian borderline tumors. We aimed at assessing the influence of different hormone therapies on this risk.......Little is known about the influence of postmenopausal hormone therapy on the risk of ovarian borderline tumors. We aimed at assessing the influence of different hormone therapies on this risk....

  10. Nongenomic actions of thyroid hormone.

    Davis, Paul J; Goglia, Fernando; Leonard, Jack L


    The nongenomic actions of thyroid hormone begin at receptors in the plasma membrane, mitochondria or cytoplasm. These receptors can share structural homologies with nuclear thyroid hormone receptors (TRs) that mediate transcriptional actions of T3, or have no homologies with TR, such as the plasma membrane receptor on integrin αvβ3. Nongenomic actions initiated at the plasma membrane by T4 via integrin αvβ3 can induce gene expression that affects angiogenesis and cell proliferation, therefore, both nongenomic and genomic effects can overlap in the nucleus. In the cytoplasm, a truncated TRα isoform mediates T4-dependent regulation of intracellular microfilament organization, contributing to cell and tissue structure. p30 TRα1 is another shortened TR isoform found at the plasma membrane that binds T3 and mediates nongenomic hormonal effects in bone cells. T3 and 3,5-diiodo-L-thyronine are important to the complex nongenomic regulation of cellular respiration in mitochondria. Thus, nongenomic actions expand the repertoire of cellular events controlled by thyroid hormone and can modulate TR-dependent nuclear events. Here, we review the experimental approaches required to define nongenomic actions of the hormone, enumerate the known nongenomic effects of the hormone and their molecular basis, and discuss the possible physiological or pathophysiological consequences of these actions. PMID:26668118

  11. Growth Hormone and Endocrinopathies

    This is an analysis of 39 patients studied at the Yonsei Medical Center from January, 1976 to March 1979. Of these 35 patient were suspected of having hypothalamic insufficiency and subjected to the L-Dopa stimulation test to observe growth hormone secretary function while four acromegaly patient received the glucose loading test and L-Dopa stimulation test. The results are as follows: 1) The basal level of GH in the various disease was as follows: a) The basal level was lower than the control level but was not statistically significant b) In diabetes the mean value tended to higher than the control level but was not significant statistically c) In all four acromegaly patients the GH level was significantly higher than the control level 2) Of 13 patients with diabetes, nine had diabetic retinopathy, and of those nine, six showed increased L-Dopa response. However, of the four non retinopathic DM patients, only one showed increased response to L-Dopa. 3) Two patients out of ten with Sheehan's syndrome responded to L-Dopa stimulation. 4) One Patient of eight with pituitary chromophobe adenoma responded to L-Dopa stimulation. 5) Four acromegaly patients revealed 3 acidophilic adenoma and one chromophobe adenoma histologically. Of patients receiving the L-Dopa stimulation test. Two showed a paradoxical response. Two patients who received the glucose loading test showed suppressed response. 6) Of two craniopharyngioma patients, one showed increased GH response after L-Dopa stimulation. Increased response of GH after L-Dopa stimulation was seen in one two craniopharyngioma patients and also in one of two patients with short structure.

  12. Thyroid Hormone Deiodinases and Cancer

    Antonio eBianco


    Full Text Available Deiodinases constitute a group of thioredoxin-containing selenoenzymes that play an important function in thyroid hormone homeostasis and control of thyroid hormone action. There are three known deiodinases: D1 and D2 activate the pro-hormone thyroxine (T4 to T3, the most active form of thyroid hormone, while D3 inactivates thyroid hormone and terminates T3 action. A number of studies indicate that deiodinase expression is altered in several types of cancers, suggesting that (i they may represent a useful cancer marker and/or (ii could play a role in modulating cell proliferation - in different settings thyroid hormone modulates cell proliferation. For example, although D2 is minimally expressed in human and rodent skeletal muscle, its expression level in rhabdomyosarcoma (RMS-13 cells is 3-4 fold higher. In basal cell carcinoma (BCC cells, sonic hedgehog (Shh-induced cell proliferation is accompanied by induction of D3 and inactivation of D2. Interestingly a 5-fold reduction in the growth of BCC in nude mice was observed if D3 expression was knocked down. A decrease in D1 activity has been described in renal clear cell carcinoma, primary liver cancer, lung cancer, and some pituitary tumors, while in breast cancer cells and tissue there is an increase in D1 activity. Furthermore D1 mRNA and activity were found to be decreased in papillary thyroid cancer while D1 and D2 activities were significantly higher in follicular thyroid cancer tissue, in follicular adenoma and in anaplastic thyroid cancer. It is conceivable that understanding how deiodinase dysregulation in tumor cells affect thyroid hormone signaling and possibly interfere with tumor progression could lead to new antineoplastic approaches.

  13. Alternatives of menopausal hormone therapy

    Kutlešić Ranko M.


    Full Text Available Introduction. It has been generally accepted that the benefits of menopausal hormone therapy outweigh the risks, but there are still some concerns about the administration of menopausal hormone therapy, which has introduced alternative treatments. Pharmacological Alternatives. Central alpha-2 agonist clonidine is only marginally more effective than placebo, and significantly less effective than estrogen. Antiepileptic drug gabapentin reduces hot flashes; however, it is less effective than estrogen. Selective serotonin reuptake inhibitors (paroxetine and fluoxetine and selective noradrenaline reuptake inhibitors (venlafaxine reduce vasomotor symptoms and improve depression, anxiety and sleep. Results of studies about dehydroepiandrosterone effects on menopausal symptoms are inconsistent and additional investigations are needed. Non-Pharmacological Alternatives. Stellatum ganglion blockade is a successful treatment for reducing vasomotor symptoms in patients with contraindications for menopausal hormone therapy. Efficacy of acupuncture, homeopathy and reflexology should be proved by adequate studies. Phytoestrogens could reduce vasomotor symptoms but to a lesser extent than conventional menopausal hormone therapy. However, they have not been proved yet to provide cardiovascular protection and prevention of osteoporosis, nor they could be recommended instead of traditional menopausal hormone therapy. There is a concern about their undesirable effects. Adequate diet, unchanging body weight within ideal values and adequate physical activities have beneficial long-term effects, first of all on preservation of bone density. Alternatives for Atrophic Changes of Vaginal Epithelium. Menopausal symptoms resulting from vaginal atrophy could be resolved by use of hydrophilic preparations, lubricants and topical lidocaine cream or 4% lidocaine water solution for dyspareunia. Conclusion. If there are contrain­dications to menopausal hormone therapy or

  14. Is dehydroepiandrosterone a hormone?

    Labrie, F; Luu-The, V; Bélanger, A; Lin, S-X; Simard, J; Pelletier, G; Labrie, C


    Dehydroepiandrosterone (DHEA) is not a hormone but it is a very important prohormone secreted in large amounts by the adrenals in humans and other primates, but not in lower species. It is secreted in larger quantities than cortisol and is present in the blood at concentrations only second to cholesterol. All the enzymes required to transform DHEA into androgens and/or estrogens are expressed in a cell-specific manner in a large series of peripheral target tissues, thus permitting all androgen-sensitive and estrogen-sensitive tissues to make locally and control the intracellular levels of sex steroids according to local needs. This new field of endocrinology has been called intracrinology. In women, after menopause, all estrogens and almost all androgens are made locally in peripheral tissues from DHEA which indirectly exerts effects, among others, on bone formation, adiposity, muscle, insulin and glucose metabolism, skin, libido and well-being. In men, where the secretion of androgens by the testicles continues for life, the contribution of DHEA to androgens has been best evaluated in the prostate where about 50% of androgens are made locally from DHEA. Such knowledge has led to the development of combined androgen blockade (CAB), a treatment which adds a pure anti-androgen to medical (GnRH agonist) or surgical castration in order to block the access of the androgens made locally to the androgen receptor. In fact, CAB has been the first treatment demonstrated to prolong life in advanced prostate cancer while recent data indicate that it can permit long-term control and probably cure in at least 90% of cases of localized prostate cancer. The new field of intracrinology or local formation of sex steroids from DHEA in target tissues has permitted major advances in the treatment of the two most frequent cancers, namely breast and prostate cancer, while its potential use as a physiological HRT could well provide a physiological balance of androgens and estrogens, thus

  15. Hormone therapy and ovarian cancer

    Mørch, Lina Steinrud; Løkkegaard, Ellen; Andreasen, Anne Helms;


    CONTEXT: Studies have suggested an increased risk of ovarian cancer among women taking postmenopausal hormone therapy. Data are sparse on the differential effects of formulations, regimens, and routes of administration. OBJECTIVE: To assess risk of ovarian cancer in perimenopausal and postmenopau......CONTEXT: Studies have suggested an increased risk of ovarian cancer among women taking postmenopausal hormone therapy. Data are sparse on the differential effects of formulations, regimens, and routes of administration. OBJECTIVE: To assess risk of ovarian cancer in perimenopausal and...... postmenopausal women receiving different hormone therapies. DESIGN AND SETTING: Nationwide prospective cohort study including all Danish women aged 50 through 79 years from 1995 through 2005 through individual linkage to Danish national registers. Redeemed prescription data from the National Register of...... bands included hormone exposures as time-dependent covariates. PARTICIPANTS: A total of 909,946 women without hormone-sensitive cancer or bilateral oophorectomy. MAIN OUTCOME MEASURE: Ovarian cancer. RESULTS: In an average of 8.0 years of follow-up (7.3 million women-years), 3068 incident ovarian...

  16. Radioimmunological and clinical studies with luteinizing hormone releasing hormone (LRH)

    Radioimmunoassay for Luteinizing Hormone Releasing Hormone (LRH) has been established, tested and applied. Optimal conditions for the performance with regards to incubation time, incubation temperature, concentration of antiserum and radiolabelled LRH have been established. The specificity of the LRH immunoassay was investigated. Problems with direct measurement of LRH in plasmas of radioimmunoassay are encountered. The LRH distribution in various tissues of the rat are investigated. By means of a system for continuous monitoring of LH and FSH in women the lowest effective dose of LRH causing a significant release of LH and FSH could be established. (Auth.)

  17. Stress hormones and physical activity

    Editorial Office


    Full Text Available Hormone secretion during physical activity of specific duration and intensity is part of the stress response. In a study to investigate the secretion of ß-endorphin, leucine enkephalin and other recognised stress hormones during physical exercise, blood samples were taken from fourteen (14 healthy, male athletes who competed in a 21 km roadrace. Blood samples were collected before and after completion of the race. This study shows that ß-endorphin/ß-lipotropin, leucine enkephalin, prolactin, and melatonin may be classified as stress hormones in physical activity of duration 80 to 120 minutes and intensity exceeding 75%-V0₂max. Widespread intra-individual variation in serum cortisol concentrations prevent definite conclusion. The un­expected increase in serum testosterone levels warrants further research.

  18. The interaction of growth hormone releasing hormone with other hypothalamic hormones on the release of anterior pituitary hormones.

    Looij, B J; Nieuwenhuijzen Kruseman, A C; Mudde, A H; Frölich, M; Piaditis, G P; Hodgkinson, S C; McLean, C; Grossman, A; Coy, D H; Rees, L H


    To determine whether the 29 amino-acid fragment of growth hormone releasing hormone (GHRH) can be combined with other hypothalamic releasing hormones in a single test of anterior pituitary reserve, the responses of anterior pituitary hormones to combinations of an i.v. bolus of GHRH(1-29)NH2 or saline with an i.v. bolus of either LH releasing hormone (LHRH) plus TRH, ovine CRH(oCRH) or saline were studied. Each infusion of GHRH(1-29)NH2 resulted in a rapid increment of the plasma GH value. Infusion of GHRH(1-29)NH2 also caused a small and transient rise in plasma PRL, but no change in the integrated PRL response. The combination of GHRH(1-29)NH2 with LHRH plus TRH caused a larger increment of peak and integrated plasma TSH levels than LHRH plus TRH alone. GHRH(1-29)NH2 did not affect the release of other anterior pituitary hormones after infusion with oCRH or LHRH plus TRH. Because of the finding of potentiation of the TSH-releasing activity of LHRH plus TRH by GHRH(1-29)NH2, the study was extended to the investigation of TSH release after infusion of TRH in combination with either GHRH(1-29)NH2 or GHRH(1-40). In this study the combination of TRH with both GHRH preparations also caused a larger increment of the peak and integrated plasma TSH levels than TRH alone. It is concluded that GHRH(1-29)NH2 possesses moderate PRL-releasing activity apart from GH-releasing activity. In addition, GHRH potentiates the TSH-releasing activity of TRH.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:2871949

  19. Hormonal evaluation in erectile dysfunction

    Selahattin Çalışkan


    Full Text Available Objective: Erectile dysfunction (ED is defined as the inability to achieve and maintain an erection sufficient to permit satisfactory sexual intercourse. In this study, we evaluated the relationship between ED and hormonal abnormalities. Material and methods: We evaluated 178 patients between the ages of 25 and 85 years old. Medical histories and details were collected, and the IIEF question test was completed by all patients. After the basic evaluation, serum total testosterone, thyroid stimulating hormone (TSH, prolactin, follicle stimulating hormone (FSH and luteinizing hormone (LH levels were measured.Results: The mean age of the patients and IIEF scores were 50.5±12.3 and 12.8±6.13, respectively. The mean testosterone, prolactin, TSH, LH and FSH were 426±152 ng/dL, 15.8±45.6 ng/mL, 1.56±1.2 micro IU/mL, 5.5±4.3 m IU/mL and 7.7±6.9 m IU/mL, respectively. Two patients had abnormal TSH levels, and 27 patients had abnormal LH levels. Abnormal FSH levels were detected in 6 patients. Eight patients had abnormal testosterone levels, and twenty had abnormal prolactin levels.Conclusion: ED is an illness that affects older men, and multiple factors cause this illness. Hormonal abnormalities are one of these factors that can be corrected. When appropriate, hormone levels should be measured and treated in patients who present with ED.

  20. Hormonal interaction in diabetic pregnancy

    Serum glucose, human placental lactogen (HPL), prolactin (PRL), estradiol (E2), progesterone (P), cortisol and human growth hormone (HGH) were determined in nondiabetic (19 cases) and diabetic (19 cases) pregnant women during the 32nd and 36th week of gestation. Significant elevation of HPL, PRL, HGH and cortisol was found in the diabetic pregnant women during the 32nd week while E2 and P were not significantly changed from the corresponding levels in the nondiabetic group. One can conclude that the changes in the hormonal pattern during gestation may induce carbohydrate intolerance observed in diabetic pregnancies. (author)

  1. Hormonal interaction in diabetic pregnancy

    Hafiez, A.R.A.; Abdel-Hafez, M.A.; Osman, E.A. (Cairo Univ. (Egypt)); Ibrahim, M.S. (Al-Azhar Univ., Cairo (Egypt))


    Serum glucose, human placental lactogen (HPL), prolactin (PRL), estradiol (E/sub 2/), progesterone (P), cortisol and human growth hormone (HGH) were determined in nondiabetic (19 cases) and diabetic (19 cases) pregnant women during the 32nd and 36th week of gestation. Significant elevation of HPL, PRL, HGH and cortisol was found in the diabetic pregnant women during the 32nd week while E/sub 2/ and P were not significantly changed from the corresponding levels in the nondiabetic group. One can conclude that the changes in the hormonal pattern during gestation may induce carbohydrate intolerance observed in diabetic pregnancies.

  2. Investigation of suspected growth hormone deficiency

    Milner, R. D. G.; Burns, E C


    This paper describes views of the Health Services Human Growth Hormone Committee on how a child suspected of growth hormone deficiency should be investigated in a district general hospital or in a regional growth centre.

  3. Hormone May Be Linked to Teenage Obesity

    ... 159014.html Hormone May Be Linked to Teenage Obesity Researchers suspect low levels of spexin might play ... reduced levels of this hormone in adults with obesity. Overall, our findings suggest spexin may play a ...

  4. Hormone May Be Linked to Teenage Obesity

    ... page: Hormone May Be Linked to Teenage Obesity Researchers suspect ... may have lower levels of a weight-regulating hormone than normal-weight teens, a new study says. " ...

  5. Gastrointestinal hormone research - with a Scandinavian annotation

    Rehfeld, Jens F


    Gastrointestinal hormones are peptides released from neuroendocrine cells in the digestive tract. More than 30 hormone genes are currently known to be expressed in the gut, which makes it the largest hormone-producing organ in the body. Modern biology makes it feasible to conceive the hormones...... under five headings: The structural homology groups a majority of the hormones into nine families, each of which is assumed to originate from one ancestral gene. The individual hormone gene often has multiple phenotypes due to alternative splicing, tandem organization or differentiated posttranslational...... maturation of the prohormone. By a combination of these mechanisms, more than 100 different hormonally active peptides are released from the gut. Gut hormone genes are also widely expressed outside the gut, some only in extraintestinal endocrine cells and cerebral or peripheral neurons but others also in...

  6. Hormonal contraceptives and venous thrombosis

    Stegeman, Berendina Hendrika (Bernardine)


    Oral contraceptive use is associated with venous thrombosis. However, the mechanism behind this remains unclear. The aim of this thesis was to evaluate genetic variation in the first-pass metabolism of contraceptives, to identify the clinical implications of hormonal contraceptive use after a thromb

  7. Sex hormone binding globulin phenotypes

    Cornelisse, M M; Bennett, Patrick; Christiansen, M;


    Human sex hormone binding globulin (SHBG) is encoded by a normal and a variant allele. The resulting SHBG phenotypes (the homozygous normal SHBG, the heterozygous SHBG and the homozygous variant SHBG phenotype) can be distinguished by their electrophoretic patterns. We developed a novel detection...

  8. Parathyroid hormone and bone healing

    Ellegaard, M; Jørgensen, N R; Schwarz, P


    pharmacological treatments are available. There is therefore an unmet need for medications that can stimulate bone healing. Parathyroid hormone (PTH) is the first bone anabolic drug approved for the treatment of osteoporosis, and intriguingly a number of animal studies suggest that PTH could be beneficial in the...

  9. Cardiac hypertrophy and thyroid hormone signaling

    Dillmann, Wolfgang


    Thyroid hormone exerts a large number of influences on the cardiovascular system. Increased thyroid hormone action increases the force and speed of systolic contraction and the speed of diastolic relaxation and these are largely beneficial effects. Furthermore, thyroid hormone has marked electrophysiological effects increasing heart rate and the propensity for atrial fibrillation and these effects are largely mal-adaptive. In addition, thyroid hormone markedly increases cardiac angiogenesis a...

  10. Cardiac hypertrophy and thyroid hormone signaling

    Dillmann, Wolfgang


    Thyroid hormone exerts a large number of influences on the cardiovascular system. Increased thyroid hormone action increases the force and speed of systolic contraction and the speed of diastolic relaxation and these are largely beneficial effects. Furthermore, thyroid hormone has marked electrophysiological effects increasing heart rate and the propensity for atrial fibrillation and these effects are largely mal-adaptive. In addition, thyroid hormone markedly increases cardiac angiogenesis a...