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Sample records for added punica granatum

  1. Constituents of the flowers of Punica granatum.

    Wang, Rufeng; Wei Wang; Wang, Liang; Liu, Ruining; Yi Ding; Du, Lijun

    2006-12-01

    A new polyphenol compound named pomegranatate (1), together with, ellagic acid, 3,3',4'-tri-O-methylellagic acid, ethyl brevifolincarboxylate, urolic and maslinic acids, and daucosterol were isolated from the ethanolic extract of the flowers of Punica granatum. The structure of compound 1 was determined by spectroscopic analysis. Maslinic acid exhibited antioxidant activity, evaluated by measurement of LDL susceptibility to oxidation. PMID:16887296

  2. Anthocyanins from pomegranate (Punica granatum L.)

    Cabrita, Luís; Neves, Higuinaldo Chaves das

    2002-01-01

    The anthocyanin contents in the fruits from three cultivars of Punica granatum L. (asseria, mollar and negral) was studied. Six anthocyanins were isolated from the red seeds of pomegranate fruits using a combination of Sephadex LH-20 column chromatography and reverse-phase semi-preparative HPLC. On the basis of chromatographic and spectroscopic techniques, they were identified as the 3-monoglucosides and 3,5-diglucosides of delphinidin, cyanidin and pelargonidin. Total anthocyanin content in...

  3. DETERMINATION OF ANTIBACTERIAL ACTIVITY OF PUNICA GRANATUM FRUIT

    P. P. Lasure*, N. M. Munot and S. S. Lawande

    2012-01-01

    Punica granatum L. belongs to the family Punicaceae, which is originating from the Middle East, extending throughout the Mediterranean, eastward to China and India. Punica granatum has been used extensively as a traditional medicine in many countries for the treatment of dysentery, diarrhea, helminthiasis, acidosis, hemorrhage and respiratory pathologies. In addition, P. granatum is reported to have antioxidant, anti-atherosclerotic and antiviral properties. The dried pomegranate peels (20gms...

  4. Effect of added Punica granatum peel fruits and Nigella sativa seeds on immunology and performance of suckling buffalo calves

    The use of feed additives is considered as a good source to improve the immunity status of newborn calves instead of chemical products because the residuals of chemical products may have side effects on human health on the long-run. Punica granatum fruits peel (Pg-p) has been shown to possess a significant antioxidant activity also valued for their therapeutic properties and used to remove intestinal tapeworm. On the other hand, seeds of Nigella sativa (NS) are rich in fatty acids (Linoleic and Linolenic acid), essential fatty acids and non-starch polysaccharide. Recently, clinical and animal studies have shown that extract of the NS seeds have many therapeutic effects such as immunomodilative, antibacterial, hypotensive, hepatoprotective, and antidiabetic effects. Thirty-six suckling buffalo claves (18 males and 18 females) were used in this study with average live body weight 38.44 ± 0.62 kg. The calves were divided into 3 groups, each group allotted to receive one of 3 tested starters throughout 105 d. The starter was offered without additive in the control group (G1), while it was mixed with 2% Pg-p in group (G2) and with 2% Pg-p plus 3% NS in group (G3). Weekly faecal swabs were collected from the experimental animals for bacteriological examination. Immunoglobulins (Ig) and blood metabolites were determined in blood serum. Three sets of digestion trials were carried out using 3 male calves of each group that fed the same tested rations. Milk consumption was not significantly different among groups while, starter and clover hay intake was significantly (P < 0.05) higher in G3 than those in groups G1 and G2. The total weight gain achieved by group G3 was greater than that of G1 and G2 groups by 25.60% and 7.80%, respectively. Blood TP was significantly (P < 0.05) higher in G3 than that in G1. An albumin value was relatively higher in G1 compared with the treated G2 and G3. Meanwhile, blood urea nitrogen was significantly greater in G2 in comparison with G1 and

  5. A Review Study on Punica granatum L.

    Shaygannia, Erfaneh; Bahmani, Mahmoud; Zamanzad, Behnam; Rafieian-Kopaei, Mahmoud

    2016-07-01

    Punica granatum L (pomegranate) is a deciduous shrub, native to Iran. Nowadays, besides its use as a fruit, its medicinal properties have attracted the interest of researchers of many countries. Pomegranate fruit has medicinal properties such as anti-inflammatory and antibacterial activities. The pomegranate seed oil has inhibitory effect on skin and breast cancers. The pomegranate seed oil has phytoestrogenic compounds and the fruit is rich in phenolic compounds with strong antioxidant activity. Ellagic acid is one of the main components of pomegranate with phenolic structure and antioxidant activity. This review article presents the recently published findings on different aspects of this plant focusing on its medicinal properties. PMID:26232244

  6. Ultrasound assisted extraction of polyphenols from Punica granatum (Grenada) fruit

    Extraction of bioactive compounds from vegetable materials is a classical operation applied in many industrial processes. Few studies have made reference to processes to obtain extracts from Punica granatum. This fruit is generally consumed as such or as fermented juices. In Cuba, it is known as grenade and numerous studies have shown antiviral, antioxidant and antimicrobial properties. Maceration process is the main used technology in the preparation of extracts from Punica granatum fruit, being this a long and expensive process. Ultrasound-assisted extractions have been proven to significantly decreased extraction time and increased extraction yields in many vegetable materials. However, few papers report the use of this methodology in the extraction processes of Punica granatum. To evaluate an ultrasound-assisted extraction process to extract polyphenols from Punica granatum fruit

  7. Punica granatum: A New Host of Bipolaris spicifera in Morocco

    Oumama Kadri; Rachid Benkirane; Amina Ouazzani Touhami; Allal Douira

    2011-01-01

    During March 2009 in the public gardens and the University of Sciences of Kenitra city (Morocco), 60% of leaves of 100 plants of pomegranate (Punica granatum L. ’Nana’) presented foliar lesions that affecting the aesthetic appearance of this ornamental plant. Bipolaris spicifera was isolated from ten necrotic lesions of 25 leaves. The artificial inoculation of the healthy leaves of three plants of Punica granatum L. ’Nana’ by conidial suspension of the pathogen induced the same lesions to tha...

  8. Determination of Bioactive Components of Ethyl Acetate Fraction of Punica granatum Rind Extract

    J. Sangeetha; Vijayalakshmi, K.

    2011-01-01

    Punica granatum belongs to a Punicaceae family. The Punica granatum is valued as a powerful medicinal plant and used in folk medicines. Hence the present investigation was carried out to determine the possible chemical components from ethyl acetate fraction of Punica granatum rind extract by GC-MS Technique. This analysis revealed that ethyl acetate fraction of Punica granatum rind extract contain Pyrogallol (41.88%), 5-Hydroxymethylfurfural (14.10%), D-Allose (9.17%), 2-Methoxy-1, 4-Benzened...

  9. Molluscicidal activity of Punica granatum bark and Canna indica root

    Tripathi, S.M.; Singh, D.K.

    2000-01-01

    The molluscicidal activity of Punica granatum Linn. (Punicaceae) and Canna indica Linn. (Cannaceae) against the snail Lymnaea acuminata was studied. The molluscicidal activity of P. granatum bark and C. indica root was found to be both time and dose dependent. The toxicity of P. granatum bark was more pronounced than that of C. indica. The 24 h LC50 of the column-purified root of C. indica was 6.54 mg/l whereas that of the column-purified bark of P. granatum was 4.39 mg/l. The ethanol extract...

  10. Analgesic activity of various extracts of Punica granatum (Linn flowers

    Chakraborthy Guno

    2008-01-01

    Full Text Available The extracts of flowers of Punica granatum (Linn. (N.O. Family Punicaceae were investigated for analgesic activity in mice using hot plate method. The flowers of Punica granatum (Linn were collected from the local market of Mumbai, Maharashtra and were in a dried condition. The dried powdered flowers (500 gm were extracted in a soxhlet apparatus by using different solvents. Mice weighing 15-25 gm were taken for the experiment. The reaction time of animals in all the groups was noted at 30, 60 and 120 min after drug administration. All data were analyzed with Student-t test. The various extract of the flowers of Punica granatum (Linn showed significant analgesic activity at a dose of 50 mg/kg body weight. A maximum analgesic activity was found at 60 min, after drug administration, which was equivalent to the standard drug used as morphine sulphate.

  11. Isolation of Antidiabetic Principle from Fruit Rinds of Punica granatum

    Vishal Jain; G. L. Viswanatha; Manohar, D.; Shivaprasad, H. N.

    2012-01-01

    Present study was aimed to isolate and evaluate the antidiabetic activity of phytoconstituents from fruit rinds of Punica granatum. With the above objectives Valoneic acid dilactone (VAD) was isolated from methanolic fruit rind extracts of Punica granatum (MEPG) and confirmed by 1H-NMR, 13C-NMR, and mass spectral data. Antidiabetic activity was evaluated by Aldose reductase, α -amylase and PTP1B inhibition assays in in vitro and Alloxan-induced diabetes in rats was used as an in vivo model. I...

  12. Investigation of a betainic alkaloid from Punica granatum.

    Schmidt, Andreas; Mordhorst, Thorsten; Nieger, Martin

    2005-07-01

    Spectroscopic investigations reveal that a hydroquinone pyridinium alkaloid isolated from the leaves of pomegranates Punica granatum L. (X-ray) exists as a mixture of a conjugated and a cross-conjugated heterocyclic mesomeric betaine in aqueous and DMSO-d6 solution. Twofold deprotonation yields an anionic tripole. PMID:15938201

  13. Isolation and Characterization of Novel Microsatellite Markers in Pomegranate (Punica granatum L.)

    Pirseyedi, Seyed Mostafa; Valizadehghan, Sahar; Mardi, Mohsen; Ghaffari, Mohammad Reza; Mahmoodi, Parvaneh; Zahravi, Mehdi; Zeinalabedini, Mehrshad; Nekoui, Seyed Mojtaba Khayam

    2010-01-01

    Pomegranate (Punica granatum L.) has been cultivated from ancient times for its economic, ornamental and medicinal properties globally. Here, we report the isolation and characterization of 12 polymorphic microsatellite markers from a repeat-enriched genomic library of Punica granatum L. The genetic diversity of these loci was assessed in 60 genotypes of Punica granatum L. All loci were variable: the number of polymorphic alleles per locus ranged from two to five (average 2.9). The observed a...

  14. Determination of Bioactive Components of Ethyl Acetate Fraction of Punica granatum Rind Extract

    J. Sangeetha

    2011-04-01

    Full Text Available Punica granatum belongs to a Punicaceae family. The Punica granatum is valued as a powerful medicinal plant and used in folk medicines. Hence the present investigation was carried out to determine the possible chemical components from ethyl acetate fraction of Punica granatum rind extract by GC-MS Technique. This analysis revealed that ethyl acetate fraction of Punica granatum rind extract contain Pyrogallol (41.88%, 5-Hydroxymethylfurfural (14.10%, D-Allose (9.17%, 2-Methoxy-1, 4-Benzenediol (8.34% and 2, 3 Dimethylfumaric acid (3.96% justifying the use of this plant to treat many disease in folk and herbal medicine.

  15. Punica granatum: A New Host of Bipolaris spicifera in Morocco

    Oumama Kadri

    2011-01-01

    Full Text Available During March 2009 in the public gardens and the University of Sciences of Kenitra city (Morocco, 60% of leaves of 100 plants of pomegranate (Punica granatum L. ’Nana’ presented foliar lesions that affecting the aesthetic appearance of this ornamental plant. Bipolaris spicifera was isolated from ten necrotic lesions of 25 leaves. The artificial inoculation of the healthy leaves of three plants of Punica granatum L. ’Nana’ by conidial suspension of the pathogen induced the same lesions to that observed in nature. The diseased foliar surface and the total number of diseased leaves 30 days after inoculation with B. spicifera conidial suspension were 37.5% and 78. Conidia production of B. spicifera on inoculated leaves was 0.81 x 105 spore cm-2 and the fungus was re-isolated from lesions on inoculated plants. This is the first report of B. spicifera on pomegranate in Morocco.

  16. PUNICA GRANATUM ATTENUATES SCIATIC NERVE LIGATION INDUCED-NEUROPATHIC PAIN

    Ramica Sharma et al.

    2012-01-01

    The study has been designed to investigate the effect of aqueous extract of rind of Punica granatum in sciatic nerve ligation induced-neuropathic pain in rats. Surgical procedure was performed with sciatic nerve ligation to develop neuropathic pain in rats. The development of neuropathic pain was assessed by employing behaviour parameters such as hyperalgesia and allodynia. Further, the functionality of sciatic nerve was assessed using the histopathological study of myelinated and unmyelinate...

  17. PUNICA GRANATUM: A REVIEW ON PHARMACOLOGICAL AND THERAPEUTIC PROPERTIES

    Neelam Arun* and D. P. Singh

    2012-01-01

    A wide range of medicinal plant parts is used as raw drugs and they possess varied medicinal properties. The different parts used as raw drugs include root, stem, flower, fruit, twigs exudates and modified plant organs. The Punica granatum is a native shrub of occidental Asia and Mediterranean Europe that has a rich history of traditional use in medicine. For centuries, the barks, leaves, flowers, fruits, and seeds of this plant have been used to treat various diseases. The aim of this presen...

  18. PUNICA GRANATUM ATTENUATES SCIATIC NERVE LIGATION INDUCED-NEUROPATHIC PAIN

    Ramica Sharma et al.

    2012-02-01

    Full Text Available The study has been designed to investigate the effect of aqueous extract of rind of Punica granatum in sciatic nerve ligation induced-neuropathic pain in rats. Surgical procedure was performed with sciatic nerve ligation to develop neuropathic pain in rats. The development of neuropathic pain was assessed by employing behaviour parameters such as hyperalgesia and allodynia. Further, the functionality of sciatic nerve was assessed using the histopathological study of myelinated and unmyelinated fibers in sciatic nerve. Moreover, the oxidative stress was assessed by estimating serum thiobarbituric acid reactive substances (TBARS, catalase, glutathione and tissue TBARS and Superoxide dismutase (SOD. Rats exposed to sciatic nerve ligation produced marked increase in oxidative stress, which was assessed in terms of TBARS and SOD along with decrease in the level of catalase and glutathione. Moreover, it develops neuropathic pain by impairing the normal functions of myelinated and unmyelinated fibers in sciatic nerve. Treatment with aqueous extract of Punica granatum extract (100mg/kg, p.o markedly prevented sciatic nerve ligation-induced neuropathy and oxidative stress by increasing the pain threshold, by improving the functionality of sciatic nerve, by decreasing serum and tissue TBARS and tissue SOD, by increasing levels of serum glutathione and catalase. It may be concluded that Punica granatum extract reduced the oxidative stress via inhibiting p38MAPK and alleviates neuropathic symptoms and consequently improved the functionality of sciatic nerve and prevents sciatic nerve ligation–induced neuropathic pain.

  19. Molluscicidal activity of Punica granatum bark and Canna indica root.

    Tripathi, S M; Singh, D K

    2000-11-01

    The molluscicidal activity of Punica granatum Linn. (Punicaceae) and Canna indica Linn. (Cannaceae) against the snail Lymnaea acuminata was studied. The molluscicidal activity of P. granatum bark and C. indica root was found to be both time and dose dependent. The toxicity of P. granatum bark was more pronounced than that of C. indica. The 24 h LC(50) of the column-purified root of C. indica was 6.54 mg/l whereas that of the column-purified bark of P. granatum was 4.39 mg/l. The ethanol extract of P. granatum (24 h LC(50): 22.42 mg/l) was more effective than the ethanol extract of C. indica (24 h LC(50): 55.65 mg/l) in killing the test animals. P. granatum and C. indica may be used as potent molluscicides since the concentrations used to kill the snails were not toxic for the fish Colisa fasciatus, which shares the same habitat with the snail L. acuminata. PMID:11050667

  20. Molluscicidal activity of Punica granatum bark and Canna indica root

    Tripathi S.M.

    2000-01-01

    Full Text Available The molluscicidal activity of Punica granatum Linn. (Punicaceae and Canna indica Linn. (Cannaceae against the snail Lymnaea acuminata was studied. The molluscicidal activity of P. granatum bark and C. indica root was found to be both time and dose dependent. The toxicity of P. granatum bark was more pronounced than that of C. indica. The 24 h LC50 of the column-purified root of C. indica was 6.54 mg/l whereas that of the column-purified bark of P. granatum was 4.39 mg/l. The ethanol extract of P. granatum (24 h LC50: 22.42 mg/l was more effective than the ethanol extract of C. indica (24 h LC50: 55.65 mg/l in killing the test animals. P. granatum and C. indica may be used as potent molluscicides since the concentrations used to kill the snails were not toxic for the fish Colisa fasciatus, which shares the same habitat with the snail L. acuminata.

  1. Growth-Inhibitory and Apoptosis-Inducing Effects of Punica granatum L. var. spinosa (Apple Punice) on Fibrosarcoma Cell Lines

    Sineh sepehr, Koushan; Baradaran, Behzad; Mazandarani, Masoumeh; Yousefi, Bahman; Abdollahpour Alitappeh, Meghdad; Khori, Vahid

    2014-01-01

    Purpose: Punica granatum L. var. granatum (Pomegranate), an herbaceous plant found in Iran, The aim of this study was to investigate the cytotoxic effects, induction of apoptosis, and the mechanism of cell death of ethanol extract from Punica granatum L. var. spinosa on the mouse fibrosarcoma cell line, WEHI-164.

  2. Fatty acid synthase inhibitors isolated from Punica granatum L

    Jiang, He-Zhong [School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, (China); Ma, Qing-Yun; Liang, Wen-Juan; Huang, Sheng-Zhuo; Dai, Hao-Fu; Wang, Peng-Cheng; Zhao, You-Xing, E-mail: zhaoyx1011@163.com [Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou (China); Fan, Hui-Jin; Ma, Xiao-Feng, E-mail: maxiaofeng@gucas.ac.cn [College of Life Sciences, Graduate University of Chinese Academy of Sciences, Beijing (China)

    2012-05-15

    The aim of this work is the isolation of fatty acid synthase (FAS) inhibitors from the ethyl acetate extracts of fruit peels of Punica granatum L. Bioassay-guided chemical investigation of the fruit peels resulted in the isolation of seventeen compounds mainly including triterpenoids and phenolic compounds, from which one new oleanane-type triterpene (punicaone) along with fourteen known compounds were isolated for the first time from this plant. Seven isolates were evaluated for inhibitory activities of FAS and two compounds showed to be active. Particularly, flavogallonic acid exhibited strong FAS inhibitory activity with IC{sub 50} value of 10.3 {mu}mol L{sup -1}. (author)

  3. PUNICA GRANATUM: A REVIEW ON PHARMACOLOGICAL AND THERAPEUTIC PROPERTIES

    Neelam Arun* and D. P. Singh

    2012-05-01

    Full Text Available A wide range of medicinal plant parts is used as raw drugs and they possess varied medicinal properties. The different parts used as raw drugs include root, stem, flower, fruit, twigs exudates and modified plant organs. The Punica granatum is a native shrub of occidental Asia and Mediterranean Europe that has a rich history of traditional use in medicine. For centuries, the barks, leaves, flowers, fruits, and seeds of this plant have been used to treat various diseases. The aim of this present review is to throw light on the therapeutic utility of various parts of pomegranate.

  4. Fatty acid synthase inhibitors isolated from Punica granatum L

    The aim of this work is the isolation of fatty acid synthase (FAS) inhibitors from the ethyl acetate extracts of fruit peels of Punica granatum L. Bioassay-guided chemical investigation of the fruit peels resulted in the isolation of seventeen compounds mainly including triterpenoids and phenolic compounds, from which one new oleanane-type triterpene (punicaone) along with fourteen known compounds were isolated for the first time from this plant. Seven isolates were evaluated for inhibitory activities of FAS and two compounds showed to be active. Particularly, flavogallonic acid exhibited strong FAS inhibitory activity with IC50 value of 10.3 μmol L-1. (author)

  5. Topical microemulsion containing Punica granatum extract: its control over skin erythema and melanin in healthy Asian subjects

    Parveen, Rashida; Akhtar, Naveed; Mahmood, Tariq

    2014-01-01

    Introduction Punica granatum is apotent source of polyphenolic compounds with strong free radicals scavenging activity. The skin lightening effects of Punica granatum are assumed due to ellagic acid which acts by chelating copper at the active site of tyrosinase. Aim To explore a topical microemulsion (O/W) of pomegranate (Punica granatum) extract for its control on skin erythema and melanin. Material and methods Microemulsions were formulated using a polysorbate surfactant (Tween 80®) along ...

  6. Phytochemical screening and antibacterial activity of aqueous leaf extract of Punica granatum

    Manoj Kumar; Sukumar Dandapat; Manoranjan Prasad Sinha

    2015-01-01

    In the present study the leaves of Punica granatum were subjected to phytochemical screening and the impact of the leaf extract of Punica granatum was tested against Bacillus subtilis, Staphylococcus aureus and Salmonella typhi. The phytochemical analysis revealed the presence of various phytochemicals such as alkaloids, tannins, phenols, flavonoid and saponins. Agar disk diffusion method was employed to test the antibacterial activity and the result showed that the aquesous leaf extract o...

  7. Antibacterial and Antifungal Activities of Punica Granatum Peel Extracts Against Oral Pathogens

    Sh. Abdollahzadeh; RY. Mashouf; H. Mortazavi; MH. Moghaddam; N. Roozbahani; Vahedi, M.

    2011-01-01

    Objective: Punica granatum has been used for many years in folk medicine due to several purposes. The aim of the present study was to evaluate the effect of methanolic extract of Punica granatum peel (MEPGP) against Streptococcus mutans, Staphylococcus aureus, Streptococcus salivarius, Streptococcus sanguinis, Staphylococcus epidermidis, Actynomyces viscosus, Lactobacillus acidophilus and Candida albicans. Materials and Methods: In this in vitro study, the mentioned oral organisms were cultur...

  8. Punica Granatum Juice Effects on Oxidative Stress in Severe Physical Activity

    Naghizadeh-Baghi, Abbas; Mazani, Mohammad; Shadman-Fard, Ali; Nemati, Ali

    2015-01-01

    Aim: The aim of this study was to investigate Punica granatum juice effects on oxidative stress in young healthy males during severe physical activity. Methods: Our subjects were selected from healthy males at 18 - 24 years. They were enrolled and randomly distributed into control and supplemented groups. 240 ml of Punica granatum juice and tap water were given to supplement and control groups daily for two weeks, respectively. Fasting blood samples were taken at the starting and the end of t...

  9. EVALUATION OF ANTI-NOCICEPTIVE AND ANTI-INFLAMMATORY ACTIVITY OF PUNICA GRANATUM SEED EXTRACT

    Gupta Jeetendra Kumar; Sharma Sandeep Kumar; Misra Vimlesh; Patel Kanika

    2011-01-01

    The plant Punica granatum of family Punicaceae is distributed throughout India and reputed to have numerous applications in traditional medicine system. In order to justify its folkloric use in nociception and inflammation, the study was performed.In this study, the extraction of Punica granatum seed extract was carried out in aqueous media. In order to explore its potency, various experimental models of anti-nociceptive and anti-inflammatory activities were taken. The oral administration of ...

  10. ORGANOLEPTIC AND WORMICIDAL EVALUATION OF VARIOUS CRUDE EXTRACTS OF POMEGRANATE (PUNICA GRANATUM) PEEL

    Sikandar Khan Sherwani; Tasveer Zahra Bokhari; Yasmeen Bibi; Syed Aneel Gilani; Shahzad Munir; Muhammad Ajmal Shah; Ikramullah

    2013-01-01

    In the present study, organoleptic evaluation of Pomegranate (Punica granatum L) peel powder was done and found pink in color having no smell and slightly bitter taste. Moreover, aqueous and methanolic extracts of Pomegranate (Punica granatum L) peel were also prepared and investigated for their anthelmintic activity using a model worm i.e. Pheretima posthuma. Three concentrations (25, 50 and 100 mg / ml) of each extracts were studied in activity, which involved the determination of time of p...

  11. Cardioprotective potential of Punica granatum extract in isoproterenol-induced myocardial infarction in Wistar rats

    Mahalaxmi Mohan; Pankaj Patankar; Prakash Ghadi; Sanjay Kasture

    2010-01-01

    Objective: To determine the protective role of Punica granatum L. (Punicaceae) seed juice extract and its butanolic fraction on heart rate, electrocardiographic patterns, vascular reactivity to catecholamines, cardiac marker enzymes, antioxidant enzymes together with morphologic and histopathological changes in isoproterenol-induced myocardial infarction in male Wistar rats. Materials and Methods: The effects of Punica granatum seed juice extract (100 mg/kg, p.o. and 300 mg/kg, p.o.) and but...

  12. Antibacterial Effect of Hydroalcoholic Extract of Punica granatum Linn. Petal on Common Oral Microorganisms

    Farnaz Hajifattahi; Elham Moravej-Salehi; Maryam Taheri; Arash Mahboubi; Mohammad Kamalinejad

    2016-01-01

    Objectives. This study aimed to assess the effect of hydroalcoholic extract of Punica granatum Linn. (P. granatum) petal on Streptococcus sanguinis, Streptococcus mutans, Streptococcus salivarius, Streptococcus sobrinus, and Enterococcus faecalis. Materials and Methods. In this in vitro study, P. granatum extract was prepared using powdered petals and water-ethanol solvent. Antibacterial effect of the extract, chlorhexidine (CHX), and ampicillin was evaluated on brain heart infusion agar (BHI...

  13. Isolation of Antidiabetic Principle from Fruit Rinds of Punica granatum

    Vishal Jain

    2012-01-01

    Full Text Available Present study was aimed to isolate and evaluate the antidiabetic activity of phytoconstituents from fruit rinds of Punica granatum. With the above objectives Valoneic acid dilactone (VAD was isolated from methanolic fruit rind extracts of Punica granatum (MEPG and confirmed by 1H-NMR, 13C-NMR, and mass spectral data. Antidiabetic activity was evaluated by Aldose reductase, α-amylase and PTP1B inhibition assays in in vitro and Alloxan-induced diabetes in rats was used as an in vivo model. In bioactivity studies, MEPG and VAD have showed potent antidiabetic activity in α-amylase, aldose reductase, and PTP1B inhibition assays with IC50 values of 1.02, 2.050, 26.25 μg/mL and 0.284, 0.788, 12.41 μg/mL, respectively. Furthermore, in alloxan-induced diabetes model MEPG (200 and 400 mg/kg, p.o. and VAD (10, 25, and 50 mg/kg, p.o. have showed significant and dose dependent antidiabetic activity by maintaining the blood glucose levels within the normal limits. Inline with the biochemical findings histopathology of MEPG (200 and 400 mg/kg, p.o., VAD (10, 25, and 50 mg/kg, p.o., and glibenclamide (10 mg/kg, p.o. treated animals showed significant protection against alloxan-induced pancreatic tissue damage. These findings suggest that MEPG and VAD possess significant antidiabetic activity in both in vitro and in vivo models.

  14. Antibacterial activity of plant extracts against Xanthomonas axonopodis Pv. Punicae causing bacterial blight of Pomegranate (Punica granatum L.

    Alane S K

    2016-01-01

    Full Text Available Pomegranate (Punica granatum L. is the important fruit crop cultivated throughout the world. It is famous for its refreshing fruits. The crop is affected by ‘bacterial blight’ caused by Xanthomonas axonopodis pv. punicae, which is responsible for the failure of crop. It results in the dropping of leaves as well as fruits. It is very hard to manage the disease with chemicals as well as antibiotics and farmers suffer from heavy economic losses. In the present study, aqueous, ethyl alcohol and acetone extracts of ten medicinally useful plants were used against Xanthomonas axonopodis pv. punicae in vitro. The plant extracts showed antibacterial activity and caused inhibition of growth of Xanthomonas axonopodis pv. Punicae. Among them, Mentha spicata, Murraya Koeninngi, Allium sativum and Tridax procumbens caused maximum inhibition of the test bacterium.

  15. Analgesic activity of various extracts of Punica granatum (Linn) flowers

    Chakraborthy Guno

    2008-01-01

    The extracts of flowers of Punica granatum (Linn). (N.O. Family Punicaceae) were investigated for analgesic activity in mice using hot plate method. The flowers of Punica granatum (Linn) were collected from the local market of Mumbai, Maharashtra and were in a dried condition. The dried powdered flowers (500 gm) were extracted in a soxhlet apparatus by using different solvents. Mice weighing 15-25 gm were taken for the experiment. The reaction time of animals in all the groups was noted at 30...

  16. Bioactivity of Nonedible Parts of Punica granatum L.: A Potential Source of Functional Ingredients

    Nawraj Rummun; Jhoti Somanah; Srishti Ramsaha; Theeshan Bahorun; Vidushi S. Neergheen-Bhujun

    2013-01-01

    Punica granatum L. has a long standing culinary and medicinal traditional use in Mauritius. This prompted a comparative study to determine the bioefficacy of the flower, peel, leaf, stem, and seed extracts of the Mauritian P. granatum. The flower and peel extracts resulting from organic solvent extraction exhibited strong antioxidant activities which correlated with the high levels of total phenolics, flavonoids, and proanthocyanidins. The peel extract had the most potent scavenging capacity ...

  17. Protective Effect of Punica granatum on Ferric Nitrilotriacetate-Induced Toxicity in Oreochromis niloticus

    Piya KOSAI; Wipaphorn JAIKUA; Jiraungkoorskul, Kanitta; Wannee JIRAUNGKOORSKUL

    2015-01-01

    This study evaluates the efficiency of a dietary supplement of Punica granatum in reducing the toxicity of ferric nitrilotriacetate in Oreochromis niloticus, with emphasis on histopathological analysis. Fish were given a dietary supplement in doses of 0 (groups 1 and 2) and 60 mg g-1 of P. granatum (groups 3 and 4) for 28 days. After a time, fish in groups 2 and 4 received intraperitoneal injections of 9 mg kg-1 of ferric nitrilotriacetate. 24 hours after injection, histopathological lesions ...

  18. A Review on Antihyperglycemic and Antihepatoprotective Activity of Eco-Friendly Punica granatum Peel Waste

    Sushil Kumar Middha; Veena Pande; Talambedu Usha

    2013-01-01

    Over the past decade, pomegranate (Punica granatum) is entitled as a wonder fruit because of its voluminous pharmacological properties. In 1830, P. granatum fruit was first recognized in United States Pharmacopeia; the Philadelphia edition introduced the rind of the fruit, the New York edition the bark of the root and further 1890 edition the stem bark was introduced. There are significant efforts and progress made in establishing the pharmacological mechanisms of peel (pericarp or rind) and ...

  19. Molecular genetic diversity of Punica granatum L. (pomegranate) as revealed by microsatellite DNA markers

    Pomegranate (Punica granatum L.) is one of the oldest known edible fruits and more and more it arouse interest of scientific community given its numerous biological activities. However, information about its genetic resources and characterization using reliable molecular markers are still scarce. In...

  20. EVALUTION OF ANTIULCER ACTIVITY OF THE FLOWERS OF PUNICA GRANATUM EXPERIMENTAL ANIMALS

    Jabbar Zoobi; Ali Mohd

    2011-01-01

    Punica granatum L. (Punicaceae), commonly called pomegranate, is a shrub growing in the warm valleys and outer hills of the Himalayas and cultivated throughout India. The plant has been used in traditional system of medicine as antiulcerogenic, antidirrhoeal, antiworm, anticancer and neuroprotective drug.

  1. Cardioprotective potential of Punica granatum extract in isoproterenol-induced myocardial infarction in Wistar rats

    Mahalaxmi Mohan

    2010-01-01

    Full Text Available Objective: To determine the protective role of Punica granatum L. (Punicaceae seed juice extract and its butanolic fraction on heart rate, electrocardiographic patterns, vascular reactivity to catecholamines, cardiac marker enzymes, antioxidant enzymes together with morphologic and histopathological changes in isoproterenol-induced myocardial infarction in male Wistar rats. Materials and Methods: The effects of Punica granatum seed juice extract (100 mg/kg, p.o. and 300 mg/kg, p.o. and butanolic fraction of Punica granatum seed juice extract (100 mg/kg., p.o. on cardiac parameters were studied. Isoproterenol hydrochloride was used to induce myocardial infarction in Wistar rats. At the end of the experiment, heart rate, ECG, pressure rate index and cardiac marker enzyme levels were assessed. Results: Rats treated with isoproterenol (85 mg/kg, administered subcutaneously twice at an interval of 24 h showed a significant increase in heart rate, ST elevation in ECG, pressure rate index and a significant increase in the levels of cardiac marker enzymes- lactate dehydrogenase, and creatine kinase in serum. Isoproterenol significantly reduced superoxide dismutase and catalase activity and increased vascular reactivity to various catecholamines. Pretreatment with PJ (100 mg/kg, p.o. and 300 mg/kg, p.o. and B-PJ (100 mg/kg., p.o. for a period of 21 days significantly inhibited the effects of ISO on heart rate, PRI, ECG patterns, levels of LDH, CK, SOD, CAT, and vascular reactivity changes. Treatment with PJ (100 mg/kg and 300 mg/kg and B-PJ (100 mg/kg., p.o. alone did not alter any of the parameters as compared to vehicle-treated Wistar rats. Punica granatum-treated animals showed a lesser degree of cellular infiltration in histopathological studies. Conclusion: Punica granatum ameliorates cardiotoxic effects of isoproterenol and may be of value in the treatment of MI.

  2. Evaluation of roselle (Hibiscus sabdariffa) leaf and pomegranate (Punica granatum) fruit rind for activity against Meloidogyne incognita

    Pomegranate (Punica granatum) fruit and roselle (Hibiscus sabdariffa) leaves have been used in traditional medicine, including as anthelmintics. Methanolic extracts from these plants were investigated for activity against the southern root-knot nematode (RKN) Meloidogyne incognita. Dried, ground p...

  3. In Vitro and In Vivo Antibacterial Activity of Punica granatum Peel Ethanol Extract against Salmonella

    Jung-Rae Rho; Dong-Won Shin; Hun-Soo Kim; Hyun Park; Dong-Hwan Sohn; Min-San Kim; Obiang-Obounou Brice; You-Chang Oh; Hee-Sung Chae; Young-Seob Lee; Ok-Hwa Kang; Jang-Gi Choi; Dong-Yeul Kwon

    2011-01-01

    Punica granatum is commonly used in Korea as a traditional medicine for the treatment of pathogenic bacteria. In this study, we investigated the in vitro and in vivo antimicrobial activity of P. granatum peel EtOH extract (PGPE) against 16 strains of Salmonella. The minimal inhibitory concentrations of PGPE were in the range of 62.5–1000 x03BCg mL-1. In addition, the in vivo antibacterial activity of the PGPE extract was examined in a S. typhimurium infection mouse model. Mice were initially ...

  4. EVALUTION OF ANTI-INFLAMMATORY AND ANALGESIC ACTIVITY OF PUNICA GRANATUM LINN

    Priyanka Bagri; Mohd. Ali; Vidhu Aeri; Shahnaz Sultana; Malay Bhowmik

    2010-01-01

    The aqueous-ethanolic (50%) extracts of fruit rind (PGR), flower (PGF), and leaves (PGL) of Punica granatum were examined for its oral anti-inflammatory and analgesic activities at the doses of 150, 250 and 500 mg/kg body weight. Oral pretreatment with the dried extracts of P. granatum produced statistically significant and dose dependent inhibition of edema induced by carrageenan at all doses when compared to the control groups. The highest activity was shown in the PGR that at 500 mg/kg p.o...

  5. Punica granatum: A review on its potential role in treating periodontal disease.

    Prasad, Divyashree; Kunnaiah, Ravi

    2014-07-01

    A wide variety of plants possess enormous treasure of medicinal value. Majority of these medicinal plants have been used to treat various systemic conditions successfully. Over the recent years, use of these medicinal plants has resurfaced to treat oral conditions. Among the oral conditions, periodontal disease remains one of the most common. Alternative and preventive options has become the need of the hour in order to overcome the adverse effects of the antimicrobial agents used in large as an adjunct to mainstream periodontal treatment. Punica granatum (Pomegranate) is one of the oldest edible fruit which has a long history as a medicinal fruit. This review is an attempt to highlight the potential of Punica granatum as a preventive and therapeutic aid to periodontal disease. PMID:25210254

  6. EVALUATION OF ANTI-NOCICEPTIVE AND ANTI-INFLAMMATORY ACTIVITY OF PUNICA GRANATUM SEED EXTRACT

    Gupta Jeetendra Kumar

    2011-12-01

    Full Text Available The plant Punica granatum of family Punicaceae is distributed throughout India and reputed to have numerous applications in traditional medicine system. In order to justify its folkloric use in nociception and inflammation, the study was performed.In this study, the extraction of Punica granatum seed extract was carried out in aqueous media. In order to explore its potency, various experimental models of anti-nociceptive and anti-inflammatory activities were taken. The oral administration of the extract 100mg and 200mg per kg body weight showed significant pharmacological action. Furthermore the anti-ulcer activity was carried out with the help of Indomethacin induced ulceration model using Mesoprostol as standard drug and it showed no ulcerogenic effect in wistar albino rats.Overall, the extract was found to be significant anti-nociceptive and anti-inflammatory activity with no ulcerogenic adverse effect.

  7. Atividade citotóxica e antiangiogênica de Punica granatum L., Punicaceae Cytotoxic and antiangiogenic activities of Punica granatum L., Punicaceae

    Ligianne P. Oliveira; Renata C. Pinheiro; Marcelo S. Vieira; José Realino de Paula; Maria Teresa F. Bara; Marize C. Valadares

    2010-01-01

    Punica granatum L., Punicaceae, amplamente usada no Brasil, foi avaliada quanto ao seu potencial antitumoral in vitroe in vivo. Investigou-se in vitro a citotoxicidade do extrato etanólico do fruto e folha da P. granatumutilizando células K-562 e células do Tumor Ascítico de Ehrlich (TAE), pelos métodos de redução do MTT e exclusão do azul de tripano. Nos estudos in vivoavaliou-se o aumento da sobrevida de animais portadores do TAE e tratados, por via oral, com diferentes doses dos extratos e...

  8. Lipid composition of seed oils of different pomegranate (Punica granatum L.) cultivars from Spain

    Luana Fernandes; Pereira, José A.; Isabel Lopéz-Cortés; Salazar, Domingo M.; Elsa Ramalhosa; Susana Casal

    2015-01-01

    Pomegranate (Punica granatum L.) is an ancient fruit tree traditionally cultivated in the Near and Middle East. Presently, its most important growing regions include Afghanistan, Iran, Israel, USA, Italy and Spain, the latter country the largest European exporter. The pomegranate fruit can be divided into several anatomical compartments: outside peel, inside peel, and arils (pulp and seeds), the last part being usually used for consumption as is or for juice, jams and jellies production. Even...

  9. Efektivitas Ekstrak Kulit Buah Delima (Punica Granatum L.) Terhadap Bakteri Aggregatibacter Actinomycetemcomitans Secara In Vitro

    Fitri, Arisma Dwita

    2015-01-01

    Aggregatibacter actinomycetemcomintans (Aa) is one type of pathogenic bacteria that cause periodontal disease found in plaque. The occurance of resistance of microorganisms to synthetic antibiotics and the resulting side effects of herbal ingredients make a good alternative to overcome these weaknesses. Pomegranate (Punica granatum L.) is known to have a wide range of therapeutic properties that antimicrobial, antioxidant, anticarcinogenic and antiinflammatory activity. The aim of this study ...

  10. Protective effects of pomegranate (Punica granatum) juice on testes against carbon tetrachloride intoxication in rats

    Ebtesam M Al-Olayan; El-Khadragy, Manal F.; Metwally, Dina M; Ahmed E. Abdel Moneim

    2014-01-01

    Background Pomegranate fruit has been extensively used as a natural medicine in many cultures. The present study was aimed at evaluating the protective effects of pomegranate (Punica granatum) juice against carbon tetrachloride (CCl4)-induced oxidative stress and testes injury in adult Wistar rats. Methods Twenty eight Wistar albino male rats were divided equally into 4 groups for the assessment of protective potential of pomegranate juice. Rats of group I (control) received only vehicles and...

  11. Penetapan kadar kalium dan fosfor dalam buah delima merah (Punica granatum L.)

    Pahrianisa, Juli

    2016-01-01

    Pomegranate (Punica granatum L.) is a plant of the punicaceae, which contains a variety of nutrients, especially minerals are potassium and phosphorus, which can prevent bone damage. From the results of the analysis can be a significant difference between the levels of calcium and phosphorus in red pomegranate’s fruit local and import also in seed and seedless pomegranate. Determination of potassium, phosphorus in pomegranate local and pomegranate import by atomic absorption spectrophotome...

  12. Beneficial effect of Punica granatum peel extract on murine malaria-induced spleen injury

    Mubaraki, Murad A.; Hafiz, Taghreed A.; Dkhil, Mohamed A.; Al-Quraishy, Saleh

    2016-01-01

    Background Multiple drug-resistant malaria parasites have been widely detected, which has encouraged research studies focused on discovering alternative therapies. Medicinal plants such as pomegranate, Punica granatum, have been proven to exhibit antiprotozoal effects and therefore, we examined its effects on murine malaria-induced splenic injury and oxidative stress in this study. Methods Mice were divided into three groups, a vehicle control and two groups that were infected with 106 Plasmo...

  13. Bactericidal Effect of Aqueous Extracts of the Bark of the Pomegranate (Punica granatum L.) on Bacteria

    Rabah Chadli; Aman Bouzid; Khadidja Bouzid; Hamida Nader

    2015-01-01

    This research concerns the study of antibacterial properties of different aqueous extracts of the bark of the pomegranate (Punica granatum L.). Three bacterial strains were used in this test: Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella. Very interesting bactericidal properties of aqueous extracts of the bark of the pomegranate were found on bacteria. The inhibition zones have a very large diameter up to 20 mm and the MIC and MBC are low, of the order of 0.78 mg/ml. This work ...

  14. Efektivitas Ekstrak Kulit Buah Delima (Punica granatum L.) terhadap Bakteri Porphyromonas gingivalis secara in vitro

    Shinta

    2014-01-01

    Porphyromonas gingivalis is one of the most commonly bacteria that found in periodontal disease. The use of a synthetic antimicrobial treatment of periodontal disease has many side effects. The potential of natural ingredients from plants for oral prophylaxis should be considered. Pomegranate (Punica granatum L) has been known for hundreds of years for the benefit of human health, including antimicrobial activity. Pomegranate rich in phenolic, flavonoids, tannins, and anthocyanin. The conten...

  15. Development and characterization of SSR markers for pomegranate (Punica granatum L.) using an enriched library

    Hasnaoui, Nejib; Buonamici, Anna; Sebastiani, Federico; Mars, Messaoud; Trifi, Mokhtar; Vendramin, Giovanni

    2010-01-01

    In the present work, we report the development of 11 microstallite markers (SSR) for Punica granatum. Evaluated on a set of 27 pomegranate accessions sampled in Tunisia, they displayed 25 alleles, with number of alleles per locus ranging between 1 and 4, and an observed heterozygosity from 0.037 and 0.592. This set of SSR markers can be very useful for studies dealing with genetic diversity assessment of germplasm, with cultivars/varieties fingerprinting and pedigree analysis of this economic...

  16. A NEW LANOSTANYL DIGLUCURONOSIDE FROM THE FLOWERS OF PUNICA GRANATUM L.

    Ali Mohd; Jabbar Zoobi

    2011-01-01

    One new triterpene glycoside characterized as lanastan–3β–yl–glucuronopyranosyl–(61)–glucuronopyranoside has been isolated from the flowers of Punica granatum L. (Punicaceae) along with oleanolic acid acetate, n-triacontane, n-henetriacontane, tetratriacontane, n-tetracontane, 3- epi-α-amyrin, lanostenyl glucoside, β-sitosterol glycoside, trixyloside and hydroxynaphthoquinone as the known compounds . The structures of all the phytoconstituents have been elucidated on the basis of spectra...

  17. ASSESSMENT OF ANTIMICROBIAL ACTIVITY OF PUNICA GRANATUM AGAINST ANTIBIOTIC-RESISTANT CLOSTRIDIUM PERFRINGENS TYPE (D)

    FRDOOS AL FADEL , SHAZA AL LAHAM, HASSANA CHOUR

    2015-01-01

    The search for new antibiotics and alternative products to solve the increasing number of bacterial resistance to customary antibiotics has become an urgent need. To investigate the effectiveness of the extracts prepared from different parts of Syrian Punica granatum Linn (family Punicaceae), against Clostridium perfringens type (D), which is resistant against many antibiotics, 684 samples were isolated from intestines and livers of death goats by using blood agar, and a selective agar for gr...

  18. Antioxidant activity, cito- and phototoxicity of pomegranate (Punica granatum L.) seed pulp extract

    Carla Aparecida Pedriali; Adjaci Uchoa Fernandes; Patricia Araujo dos Santos; Moisés Melquíades da Silva; Divinomar Severino; Mauricio Baptista da Silva

    2010-01-01

    In the present work, a hydroethanolic extract was prepared from the entire seeds of pomegranate [Punica granatum L. (Punicaceae)] with Cachaça, a distilled Brazilian alcoholic beverage, protected from light for an 80-hour period. The desorption curve of the seeds, presented an optimal time extraction of approximately 24 hours. The extract was divided into two samples: protected from light, (Extract 1), or not, (Extract 2). The extracts were characterized by UV-Visible absorption spectroscopy,...

  19. Protective Effects of Punica Granatum Seeds Extract Against Aging and Scopolamine Induced Cognitive Impairments in Mice

    Kumar, Sokindra; Maheshwari, Kamal Kishore; Singh, Vijender

    2008-01-01

    Dementia is one of the age related mental problems and characteristic symptom of various neurodegenerative diseases including Alzheimer's disease. This impairment probably is due to the vulnerability of the brain cells to increased oxidative stress during aging process. Many studies have shown that certain phenolic antioxidants attenuate neuronal cell death induced by oxidative stress. The present work was undertaken to assess the effect of ethanolic extract of Punica granatum seeds on cognit...

  20. Lipid Lowering Effect of Punica granatum L. Peel in High Lipid Diet Fed Male Rats

    Alireza Sadeghipour; Maryam Eidi; Ali Ilchizadeh Kavgani; Reza Ghahramani; Saleh Shahabzadeh; Ali Anissian

    2014-01-01

    Many herbal medicines have been recommended for the treatment of dyslipidemia. The antilipidemic effect of hydroethanolic extract of pomegranate peel (Punica granatum L.) was investigated in high lipid diet fed male rats. Intraperitoneally administration of pomegranate peel extract (50, 100, 200, and 300 mg/kg body weight) for 23 days on the levels of serum cholesterol, triglycerides, LDL, HDL, alkaline phosphatase (AP), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in ...

  1. ASSESSMENT OF ANTIMICROBIAL ACTIVITY OF PUNICA GRANATUM AGAINST ANTIBIOTIC-RESISTANT CLOSTRIDIUM PERFRINGENS TYPE (D

    FRDOOS AL FADEL , SHAZA AL LAHAM, HASSANA CHOUR

    2015-06-01

    Full Text Available The search for new antibiotics and alternative products to solve the increasing number of bacterial resistance to customary antibiotics has become an urgent need. To investigate the effectiveness of the extracts prepared from different parts of Syrian Punica granatum Linn (family Punicaceae, against Clostridium perfringens type (D, which is resistant against many antibiotics, 684 samples were isolated from intestines and livers of death goats by using blood agar, and a selective agar for growing of Clostridium perfringens(SPS agar. The isolated bacteria were typed by using ELISA apparatus. Many parts of Punica granatum was extracted with water, absolute alcohol, then ether by using soxhlet apparatus and rotary evaporator. The Antibiotic susceptibility testing of many antibiotics was conducted by using disc diffusion method in anaerobic atmosphere and break points method. The alcoholic extracts prepared from many parts of punica granatum (pericarp, leaves, flowers, seeds showed different antibacterial effect against Clostridium perfringens type(D,whereas the studied antibiotics had not shown any antibacterial effect, except Clindamycin which showed partial effectiveness. 

  2. Studies on the Cytotoxic Activities of Punica granatum L. var. spinosa (Apple Punice) Extract on Prostate Cell Line by Induction of Apoptosis

    Koushan Sineh Sepehr; Behzad Baradaran; Masoumeh Mazandarani; Vahid Khori; Fatemeh Zare Shahneh

    2012-01-01

    The Punica granatum L. var. granatum (pomegranate) has been demonstrated to exert antitumor effects on various types of cancer cells. The present study aimed to evaluate the medicinal herbs Punica granatum L. var. spinosa (apple punice) that are native to Iran. This study was determined to test the possible cytotoxic activity and induction of apoptosis on human prostate cell lines. The effect of ethanol extracts of the herbs on the inhibition of cell proliferation was assessed by MTT colorime...

  3. Atividade citotóxica e antiangiogênica de Punica granatum L., Punicaceae Cytotoxic and antiangiogenic activities of Punica granatum L., Punicaceae

    Ligianne P. Oliveira

    2010-05-01

    Full Text Available Punica granatum L., Punicaceae, amplamente usada no Brasil, foi avaliada quanto ao seu potencial antitumoral in vitroe in vivo. Investigou-se in vitro a citotoxicidade do extrato etanólico do fruto e folha da P. granatumutilizando células K-562 e células do Tumor Ascítico de Ehrlich (TAE, pelos métodos de redução do MTT e exclusão do azul de tripano. Nos estudos in vivoavaliou-se o aumento da sobrevida de animais portadores do TAE e tratados, por via oral, com diferentes doses dos extratos etanólicos da P. granatum(12,5; 25; 50 e 100 mg/kg por dez dias consecutivos. Além disso, nestes animais analisou-se o potencial de inibição tumoral e a atividade antiangiogênica da P. granatum. Os resultados dos estudos in vitrodemonstraram uma redução na viabilidade das células K-562 e do TAE, concentração-dependente, nos métodos investigados. Os resultados in vivo demonstraram aumento da sobrevida dos animais portadores do TAE tratados, de forma dose-dependente. Em paralelo, observou-se diminuição do número de células tumorais na cavidade peritoneal dos animais portadores e tratados. Além disto, os tratamentos empregados reduziram o padrão de vascularização da parede abdominal. Dessa forma, os dados apresentados revelaram que o extrato de P. granatumpossui atividade antitumoral in vitroe in vivo em paralelo a redução da angiogênese peritoneal.Punica granatum L., a plant widely used in Brazil, was tested for its antitumor and antiangiogenic activities in vitroand in vivo. In this work, the in vitrocytotoxicity was evaluated using the K-562 cell line and Ehrlich ascites tumour cells, by MTT tetrazolium reduction test and the trypan blue exclusion test. In vivostudies investigated the increase in the survival time of Ehrlich tumour-bearing mice after treatment with different doses of Punica granatumL. ethanol extract (12.5; 25; 50 e and 100 mg/kg, by gavages, for ten consecutive days. In addition, we also investigated the tumour

  4. Pharmacological investigations of Punica granatum in glycerol-induced acute renal failure in rats

    Amrit Pal Singh

    2011-01-01

    Full Text Available Objective : The present study was designed to investigate the ameliorative potential and possible mechanism of hydroalcoholic extract of flowers of P. granatum in glycerol-induced acute renal failure (ARF in rats. Materials and Methods : The rats were subjected to rhabdomyolytic ARF by single intramuscular injection of hypertonic glycerol (50% v/v; 8 ml/kg and the animals were sacrificed after 24 hours of glycerol injection. The plasma creatinine, blood urea nitrogen, creatinine clearance, and histopathological studies were performed to assess the degree of renal injury. Results : Pretreatment with hydroalcoholic extract of flowers of P. granatum (125 and 250 mg/kg p.o. twice daily for 3 days significantly attenuated hypertonic glycerol-induced renal dysfunction in a dose-dependent manner. BADGE (Bisphenol-A-diglycidyl ether (30 mg/kg, a peroxisome proliferator-activated receptor (PPAR-γ antagonist, and N(omega-nitro-l-arginine-methyl ester (L-NAME (10, 20, and 40 mg/kg, nitric oxide synthase inhibitor, were employed to explore the mechanism of renoprotective effects of Punica granatum. Administration of BADGE (30 mg/kg and L-NAME (40 mg/kg abolished the beneficial effects of P. granatum in glycerol-induced renal dysfunction. Conclusion : Hydroalcoholic extract of flowers of P. granatum has ameliorative potential in attenuating myoglobinuric renal failure and its renoprotective effects involve activation of PPAR-γ and nitric oxide-dependent signaling pathway.

  5. Antibacterial Effect of Hydroalcoholic Extract of Punica granatum Linn. Petal on Common Oral Microorganisms.

    Hajifattahi, Farnaz; Moravej-Salehi, Elham; Taheri, Maryam; Mahboubi, Arash; Kamalinejad, Mohammad

    2016-01-01

    Objectives. This study aimed to assess the effect of hydroalcoholic extract of Punica granatum Linn. (P. granatum) petal on Streptococcus sanguinis, Streptococcus mutans, Streptococcus salivarius, Streptococcus sobrinus, and Enterococcus faecalis. Materials and Methods. In this in vitro study, P. granatum extract was prepared using powdered petals and water-ethanol solvent. Antibacterial effect of the extract, chlorhexidine (CHX), and ampicillin was evaluated on brain heart infusion agar (BHIA) using the cup-plate method. By assessing the diameter of the growth inhibition zone, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extract were determined for the above-mentioned bacteria. Results. Hydroalcoholic extract of P. granatum petal had inhibitory effects on the proliferation of all five bacterial strains with maximum effect on S. mutans with MIC and MBC of 3.9 mg/mL. The largest growth inhibition zone diameter belonged to S. sanguinis and the smallest to E. faecalis. Ampicillin and CHX had the greatest inhibitory effect on S. sanguinis. Conclusions. Hydroalcoholic extract of P. granatum had a significant antibacterial effect on common oral bacterial pathogens with maximum effect on S. mutans, which is the main microorganism responsible for dental plaque and caries. PMID:26884763

  6. Antihyperglycemic, antioxidant and hypolipidemic effect of Punica granatum L flower extract in streptozotocin induced diabetic rats

    Anusha Bhaskar; Anish Kumar

    2012-01-01

    Objective: To investigate the antihyperglycemic, hypolipidemic and antioxidant effects of aqueous and ethanolic flower extracts of Punica granatum (P. granatum) in streptozotocin (STZ) induced diabetes mellitus in wistar rats. Methods: Antihyperglycemic, hypolipidemic and antioxidant effect of P. granatum aqueous (PGFAet) and ethanolic extracts (PGFEet) at doses of 200 and 400 mg/kg bw was evaluated in streptozotocin induced diabetic rats. Glycosylated hemoglobin, plasma insulin, cholesterol and lipoprotein levels, lipid peroxide levels and the antioxidant enzyme levels were determined. Results: The administration of the extracts markedly reduced blood glucose and glycosylated hemoglobin increased the levels of plasma insulin and liver glycogen. The extract also had a hypolipidemic activity decreasing the levels of total cholesterol, LDL-cholesterol, VLDL-cholesterol and triglycerides. The levels of lipid peroxides in terms of thiobarbituric acid reactive substances (TBARS) were remarkably reduced and the activities of the enzymes SOD, CAT and GPx and GSH were increased. We also saw an increase in the activity of the enzyme glucokinase and decrease in glucose-6-phosphatase. It can be concluded that the flower extracts of P. granatum (PGFAet and PGFEet) has significant restorative effect on the blood glucose, hyperlipidemia and oxidative stress. Conclusions: It can be concluded from the studies that the ethanolic extracts of the flowers of P. granatum at dosage 400 mg/kg/day exhibit significant effect in lowering blood sugar and lipid levels and increasing the antioxidant enzymes when compared to the aqueous extract. Glycosylated hemoglobin levels were reduced and the extract exhibited a stimulatory effect on insulin.

  7. Antibacterial Effect of Hydroalcoholic Extract of Punica granatum Linn. Petal on Common Oral Microorganisms

    Farnaz Hajifattahi

    2016-01-01

    Full Text Available Objectives. This study aimed to assess the effect of hydroalcoholic extract of Punica granatum Linn. (P. granatum petal on Streptococcus sanguinis, Streptococcus mutans, Streptococcus salivarius, Streptococcus sobrinus, and Enterococcus faecalis. Materials and Methods. In this in vitro study, P. granatum extract was prepared using powdered petals and water-ethanol solvent. Antibacterial effect of the extract, chlorhexidine (CHX, and ampicillin was evaluated on brain heart infusion agar (BHIA using the cup-plate method. By assessing the diameter of the growth inhibition zone, the minimum inhibitory concentration (MIC and minimum bactericidal concentration (MBC of the extract were determined for the above-mentioned bacteria. Results. Hydroalcoholic extract of P. granatum petal had inhibitory effects on the proliferation of all five bacterial strains with maximum effect on S. mutans with MIC and MBC of 3.9 mg/mL. The largest growth inhibition zone diameter belonged to S. sanguinis and the smallest to E. faecalis. Ampicillin and CHX had the greatest inhibitory effect on S. sanguinis. Conclusions. Hydroalcoholic extract of P. granatum had a significant antibacterial effect on common oral bacterial pathogens with maximum effect on S. mutans, which is the main microorganism responsible for dental plaque and caries.

  8. Effect ofPunica granatum peel extract on learning and memory in rats

    Shalini Adiga; Prabhav Trivedi; Ravichandra V; Debashree Deb; Forum Mehta

    2010-01-01

    Objective:To evaluate potential memory enhancing effect ofPunica granatum peel extract on rats.Methods: Healthy adult male albino rats of Wistar strain were used. Each group of6 rats were administered either distilled water or50mg/kg of extract or100 mg/kg of extract for 15days and subjected to passive avoidance test or T-maze test. In the next phase rats were administered distilled water or100 mg/kg of extract for15 days and the rats were given injection diazepam before subjecting them to the tests.Results:The overall performance was better in test groups compared to control groups. Among the test groups,100 mg/kg rats performed better than50mg/kg. The effect on spatial learning parameters like mean number of alternations and mean percentage bias was more marked compared to retention testing parameters like latency. 100 mg/kg Punica extract treated group also improved performance of diazepam treated rats. Conclusions:There is a definite trend of memory improvement byPunica granatum peel with effects being more marked on spatial learning tendency and long term memory than on retention capacity.

  9. Effect of Punica granatum L. Flower Water Extract on Five Common Oral Bacteria and Bacterial Biofilm Formation on Orthodontic Wire

    Elahe Vahid Dastjerdi; Zahra Abdolazimi; Marzieh Ghazanfarian; Parisa Amdjadi; Mohammad Kamalinejad; Arash Mahboubi

    2014-01-01

    Background: Use of herbal extracts and essences as natural antibacterial compounds has become increasingly popular for the control of oral infectious diseases. Therefore, finding natural antimicrobial products with the lowest side effects seems necessary. The present study sought to assess the effect of Punica granatum L. water extract on five oral bacteria and bacterial biofilm formation on orthodontic wire. Methods: Antibacterial property of P. granatum L. water extract was primarily evalua...

  10. A Review on Antihyperglycemic and Antihepatoprotective Activity of Eco-Friendly Punica granatum Peel Waste

    Sushil Kumar Middha

    2013-01-01

    Full Text Available Over the past decade, pomegranate (Punica granatum is entitled as a wonder fruit because of its voluminous pharmacological properties. In 1830, P. granatum fruit was first recognized in United States Pharmacopeia; the Philadelphia edition introduced the rind of the fruit, the New York edition the bark of the root and further 1890 edition the stem bark was introduced. There are significant efforts and progress made in establishing the pharmacological mechanisms of peel (pericarp or rind and the individual constituents responsible for them. This review provides an insight on the phytochemical components that contribute too antihyperglycemic, hepatoprotective, antihyperlipidemic effect, and numerous other effects of wonderful, economic, and eco-friendly pomegranate peel extract (PP.

  11. Chromatographic analysis and antiproliferative potential of aqueous extracts of Punica granatum fruit peels using the Allium cepa test

    Andrielle Wouters Kuhn; Marília Tedesco; Aline Augusti Boligon; Margareth Linde Athayde; Haywood Dail Laughinghouse IV; Solange Bosio Tedesco

    2015-01-01

    Punica granatum L., locally known as romanzeira, is native to Asia but found throughout Brazil. P. granatum is used for treating inflammatory, infectious and respiratory diseases. The aim of this study was to evaluate the chromatography and genotoxicity of an aqueous extract of P. granatum (pomegranate) fruit peel using the Allium cepa L. test. The experiment set-up entailed 7 treatments: T1-distilled water, T2-tea 5 g.L-1, T3-tea 10 g.L-1, T4-glyphosate at 9.6%, T5-glyphosate with subsequent...

  12. Punica granatum and its therapeutic implications on breast carcinogenesis: A review.

    Vini, Ravindran; Sreeja, Sreeharshan

    2015-01-01

    Punica granatum has a recorded history of pharmacological properties which can be attributed to its rich reservoir of phytochemicals. Investigations in recent years have established its tremendous potential as an antitumorogenic agent against various cancers including breast cancer, which is the second leading cause of cancer-related deaths in women. The plausible role of Punica as a therapeutic agent, as an adjuvant in chemotherapy, and its dietary implications as chemopreventive agent in breast cancer have been explored. Mechanistic studies have revealed that Punica extracts and its components, individually or in combination, can modulate and target key proteins and genes involved in breast cancer. Our earlier finding also demonstrated the role of methanolic extract of pomegranate pericarp in reducing proliferation in breast cancer by binding to estrogen receptor at the same time not affecting uterine weight unlike estradiol or tamoxifen. This review analyses other plausible mechanisms of Punica in preventing the progression of breast cancer and how it can possibly be a therapeutic agent by acting at various steps of carcinogenesis including proliferation, invasion, migration, metastasis, angiogenesis, and inflammation via various molecular mechanisms. PMID:25857627

  13. Utilization of the pomegranate tree (Punica granatum L.) in the paper industry

    GÜLSOY, SEZGİN KORAY; PEKGÖZLÜ, AYBEN KILIÇ; AKTAŞ, ASLI CEREN

    2015-01-01

    In this study, the chemical composition, fiber morphology, pulp, and paper properties of the pomegranate tree (Punica granatum L.) were determined. Stem and branch wood were analyzed separately. Kraft and kraft- anthraquinone (AQ) methods were used for the pulping processes. Stem wood showed superiority to branch wood with longer, wider, thicker-walled fibers. The holocellulose, $\\alpha$-cellulose, and lignin contents of branch and stem wood were 72.98%-73.50%, 38.37%-39.92%, and 21.04%-25.29...

  14. Antibacterial activity of crude extract of Punica granatum pericarp on pathogenic Gram-negative bacilli.

    Voravuthikunchai, S.; Supavita, T.; Sirirak, T.; Panthong, K.

    2005-01-01

    The objective of this study was to investigate the effect of crude extracts of Punica granatum Linn. pericarp with 3 different solvents against pathogenic Gram-negative bacilli. Ethanolic extracts showed the antibacterial activity against all strains tested including enterohaemorrhagic Escherichia coli 4 strains (E. coli O157: H7, E. coli O26: H11, E. coli O111: NM, E. coli O22), Pseudomonas aeruginosa, Shigella boydii and Salmonella london. Inhibition zones ranged from 10.02 to 19.15 mm. Min...

  15. AMELIORATIVE EFFECT OF PUNICA GRANATUM ETHANOLIC EXTRACT IN CYCLOPHOSPHAMIDE INDUCED TESTICULAR TOXICITY IN MALE WISTAR RATS

    Divya Bhargavan; Harish Shetty; AP Krishna

    2015-01-01

    Aim: To explore the potential role of Punica granatum ethanolic extract (PGEE) in Cyclophosphamide (CP) induced testicular toxicity. Methods: Healthy male Wistar rats were allotted to 4 groups (N=6, each) Group I: Control, Group II: CP 15mg/kg twice a week, Group III: PGEE 100mg/kg, Group IV: CP and PGEE for 28 days. At the end of the treatment period, organ weight, body weight, epididymal sperm count, motility, morphology, SOD, catalase, GSH, ACP & testosterone level in the testis were evalu...

  16. A NEW LANOSTANYL DIGLUCURONOSIDE FROM THE FLOWERS OF PUNICA GRANATUM L.

    Ali Mohd

    2011-07-01

    Full Text Available One new triterpene glycoside characterized as lanastan–3β–yl–glucuronopyranosyl–(61–glucuronopyranoside has been isolated from the flowers of Punica granatum L. (Punicaceae along with oleanolic acid acetate, n-triacontane, n-henetriacontane, tetratriacontane, n-tetracontane, 3- epi-α-amyrin, lanostenyl glucoside, β-sitosterol glycoside, trixyloside and hydroxynaphthoquinone as the known compounds . The structures of all the phytoconstituents have been elucidated on the basis of spectral data analysis and chemical reactions.

  17. Punica granatum leave extract as green corrosion inhibitor for mild steel in Hydrochloric acid

    Abboud Y.; Chagraoui A.; Tanane O.; El Bouari A.; Hannache H.

    2013-01-01

    Leave of Punica granatum extract (LPGE) as green inhibitor for the corrosion of mild steel in 1M HCl solution was studied using weight-loss and potentiodynamic polarization measurements. The results obtained revealed that LPGE has fairly good inhibiting properties for mild steel corrosion in 1M HCl solution, with efficiency of around 94 % at a concentration of 1 g/l. The inhibition was of a mixed anodic–cathodic nature. The film which is formed over the metal surface was analysed by FT-IR spe...

  18. EVALUATION OF ANTI-INFLAMMATORY AND ANALGESIC ACTIVITY OF PUNICA GRANATUM LINN LEAVES

    Nain Parminder; Saini Mamta; Malik Manisha

    2011-01-01

    The methanolic extract of dried leaves of Punica granatum linn was studied for the anti-inflammatory activity in rat using carrageenan induced paw edema with plethysmometer and analgesic activity on mice by Eddy’s hot plate & tail immersion method. A preliminary phytochemical screening of leaves extract revealed the presence of alkaloids, tannins, flavonoids, and steroids. Among all the doses (200mg/kg, 400mg/kg, 600mg/kg, 800mg/kg) of methanolic extract 600mg/kg orally showed maximum signifi...

  19. Anticoagulant, antiplatelet and antianemic effects of Punica granatum (pomegranate) juice in rabbits.

    Riaz, Azra; Khan, Rafeeq A

    2016-04-01

    Pomegranate (Punica granatum L., Punicaceae) is a good source of minerals and phytochemicals with diverse pharmacological activities such as anxiolytic, antidepressant, hypoglycemic, hypolipidemic, and anti-inflammatory activities. Effects of P. granatum on blood parameters and coagulation have, however, been little studied. The aim of the study was to assess the outcome of P. granatum on coagulation and anticoagulation factors at different doses on blood samples of healthy white rabbits. Blood samples of the animals were collected twice during the study and biochemical assays were performed to assess the effect on hematological, coagulation, anticoagulation, and platelet aggregation. Significant changes were observed in erythrocytes, hemoglobin, and mean corpuscular hemoglobin concentration, while bleeding and thrombin time were also prolonged significantly. There was significant increase in protein C, thrombin antithrombin complex levels, and decrease in platelet aggregation and fibrinogen concentration, in a dose-dependent manner. The results of hematological and coagulation assays lead to the speculation about a possible antianemic and cardioprotective effect of P. granatum. PMID:26881853

  20. Apoptotic effects of non-edible parts of Punica granatum on human multiple myeloma cells.

    Kiraz, Yağmur; Neergheen-Bhujun, Vidushi S; Rummun, Nawraj; Baran, Yusuf

    2016-02-01

    Multiple myeloma is of great concern since existing therapies are unable to cure this clinical condition. Alternative therapeutic approaches are mandatory, and the use of plant extracts is considered interesting. Punica granatum and its derived products were suggested as potential anticancer agents due to the presence of bioactive compounds. Thus, polypenolic-rich extracts of the non-edible parts of P. granatum were investigated for their antiproliferative and apoptotic effects on U266 multiple myeloma cells. We demonstrated that there were dose-dependent decreases in the proliferation of U266 cells in response to P. granatum extracts. Also, exposure to the extracts triggered apoptosis with significant increases in loss of mitochondrial membrane potential in U266 cells exposed to the leaves and stem extracts, while the flower extract resulted in slight increases in loss of MMP. These results were confirmed by Annexin-V analysis. These results documented the cytotoxic and apoptotic effects of P. granatum extracts on human U266 multiple myeloma cells via disruption of mitochondrial membrane potential and increasing cell cycle arrest. The data suggest that the extracts can be envisaged in cancer chemoprevention and call for further exploration into the potential application of these plant parts. PMID:26318303

  1. Antibacterial and Antifungal Activities of Punica Granatum Peel Extracts Against Oral Pathogens

    Sh. Abdollahzadeh

    2011-03-01

    Full Text Available Objective: Punica granatum has been used for many years in folk medicine due to several purposes. The aim of the present study was to evaluate the effect of methanolic extract of Punica granatum peel (MEPGP against Streptococcus mutans, Staphylococcus aureus,Streptococcus salivarius, Streptococcus sanguinis, Staphylococcus epidermidis, Actynomyces viscosus, Lactobacillus acidophilus and Candida albicans.Materials and Methods: In this in vitro study, the mentioned oral organisms were cultured in blood agar and mueller-hinton media and then paper disks containing MEPGP at concentrations of 4 mg/ml, 8 mg/ml and 12 mg/ml were inserted on medias. The antimicrobialactivity was evaluated by agar disk diffusion method. The effects of three different concentrations of MEPGP against microorganisms were compared using one-way ANOVA and Tukey tests.Results: All concentrations of MEPGP had antibacterial activity against S. aureus and S.epidermidis. Only at concentration of 8 mg/ml and 12 mg/ml MEPGP was effective against L. acidophilus, S. mutans and S. salivarius. Furthermore; no concentrations ofMEPGP inhibited A. viscosus and C. albicans.Conclusion: This study suggests that MEPGP might be used as an antibacterial agent in controlling oral infections.

  2. Isolation and Standardization of Various Phytochemical Constituents from Methanolic Extracts of Fruit Rinds of Punica granatum

    Vishal Jain; Murugananthan G; Deepak M; GL Viswanatha; D Manohar

    2011-01-01

    AIM:The present study was undertaken to isolate and standardize the various active phytochemical constituents present in the fruit rinds of Punica granatum.METHODS:Fruit rinds of Punica granatum were dried and extracted with methanol in a static extractor; the percentage yield of the methanolic extract (MEPG) was found to be 26%; the methanolic extract was partitioned using n-butanol,ethyl acetate and water; the percentage yield of the fractions were found to be 17.16%,26.88% and 47.72% respectively.HPLC was carried out for all the fractions and the markers corresponding to the major peaks in HPLC were isolated using column chromatography.The isolated markers were characterized using UV,IR,Mass and NMR spectroscopy.RESULTS & CONCLUSION:Totally four compounds were isolated and identified as punicalin,punicalagin,gallic acid and ellagic acid based on UV,IR,Mass and NMR data,they were standardized by HPLC.

  3. EVALUTION OF ANTI-INFLAMMATORY AND ANALGESIC ACTIVITY OF PUNICA GRANATUM LINN

    Priyanka Bagri

    2010-12-01

    Full Text Available The aqueous-ethanolic (50% extracts of fruit rind (PGR, flower (PGF, and leaves (PGL of Punica granatum were examined for its oral anti-inflammatory and analgesic activities at the doses of 150, 250 and 500 mg/kg body weight. Oral pretreatment with the dried extracts of P. granatum produced statistically significant and dose dependent inhibition of edema induced by carrageenan at all doses when compared to the control groups. The highest activity was shown in the PGR that at 500 mg/kg p.o. inhibited inflammation by 82.14%. (79 % for indomethacin at 10 mg/kg. On the contrary, the aqueous-ethanolic (50 % extracts of PGF and PGL exhibited 71.42% and 67.85% inhibition, respectively, at 500 mg/kg dose. The extracts at tested doses were found to possess analgesic activity in mice against tail-flick method. These results indicated that extracts of P. granatum possessed significant anti-inflammatory and analgesic activities suggesting its potential as an anti-inflammatory agent for use in the treatment of various inflammatory diseases in traditional medicine.

  4. Antibacterial activity of crude extract of Punica granatum pericarp on pathogenic Gram-negative bacilli.

    Voravuthikunchai, S.

    2005-08-01

    Full Text Available The objective of this study was to investigate the effect of crude extracts of Punica granatum Linn. pericarp with 3 different solvents against pathogenic Gram-negative bacilli. Ethanolic extracts showed the antibacterial activity against all strains tested including enterohaemorrhagic Escherichia coli 4 strains (E. coli O157: H7, E. coli O26: H11, E. coli O111: NM, E. coli O22, Pseudomonas aeruginosa, Shigella boydii and Salmonella london. Inhibition zones ranged from 10.02 to 19.15 mm. Minimum inhibitory concentration (MIC and minimum bactericidal concentration (MBC ranged from 0.09 to 3.13 mg/ml and 3.13 to 25 mg/ml, respectively. Aqueous extract had low antibacterial activity while crude chloroform extracts had no effect on the growth of these strains. Ethyl acetate and n-butanol fractions of P. granatum pericarp demonstrated high activity with the best MIC and MBC values of 0.02 to 0.78 mg/ml and 0.19 to 6.25 mg/ml, respectively. As ethanolic extract of P. granatum was very effective against these pathogenic bacteria, further investigation on this plant species may provide alternative, but bioactive, medicines for the treatment of Gram-negative bacterial infection.

  5. Preparation and characterisation of Punica granatum pericarp aqueous extract loaded chitosan-collagen-starch membrane: role in wound healing process.

    Amal, B; Veena, B; Jayachandran, V P; Shilpa, Joy

    2015-05-01

    Engineered scaffolds made from natural biomaterials are crucial elements in tissue engineering strategies. In this study, biological scaffold like chitosan-collagen-starch membrane (CCSM) loaded with the antibacterial agent, Punica granatum pericarp aqueous extract was explored for enhanced regeneration of epithelial tissue during wound healing. Collagen was extracted from Rachycentron canadum fish skin. Membranous scaffold was prepared by mixing collagen, starch and chitosan in a fixed proportion, loaded with aqueous extract of P. granatum and its anti-pseudomonal activity was studied. Morphological characterization by SEM and mechanical property like tensile strength of the membrane were studied. Excision wound of 2 cm(2) size was induced in Guinea pig and the effect of P. granatum extract loaded CCSM in wound healing was studied. The SEM image showed deep pores in the membrane and also possessed good tensile strength. Wound surface area was reduced prominently in the experimental group with P. granatum extract loaded CCSM when compared to the group with unloaded membrane and the one with no membrane. Punica granatum extract loaded CCSM has antipseudomonal property and supported enhanced epithelial cell proliferation without leaving a scar after wound healing. This has significant therapeutic application in membranous scaffold mediated skin repair and regeneration. PMID:25893391

  6. Synthesis of gold nanoparticles using renewable Punica granatum juice and study of its catalytic activity

    Dash, Shib Shankar; Bag, Braja Gopal

    2014-01-01

    Punica granatum juice, a delicious multivitamin drink of great medicinal significance, is rich in different types of phytochemicals, such as terpenoids, alkaloids, sterols, polyphenols, sugars, fatty acids, aromatic compounds, amino acids, tocopherols, etc. We have demonstrated the use of the juice for the synthesis of gold nanoparticles (AuNPs) at room temperature under very mild conditions. The synthesis of the AuNPs was complete in few minutes and no extra stabilizing or capping agents were necessary. The size of the nanoparticles could be controlled by varying the concentration of the fruit extract. The AuNPs were characterized by surface plasmon resonance spectroscopy, high resolution transmission electron microscopy, fourier transform infrared spectroscopy and X-ray diffraction studies. Catalytic activity of the synthesized colloidal AuNPs has also been demonstrated.

  7. AMELIORATIVE EFFECT OF PUNICA GRANATUM ETHANOLIC EXTRACT IN CYCLOPHOSPHAMIDE INDUCED TESTICULAR TOXICITY IN MALE WISTAR RATS

    Divya Bhargavan

    2015-07-01

    Full Text Available Aim: To explore the potential role of Punica granatum ethanolic extract (PGEE in Cyclophosphamide (CP induced testicular toxicity. Methods: Healthy male Wistar rats were allotted to 4 groups (N=6, each Group I: Control, Group II: CP 15mg/kg twice a week, Group III: PGEE 100mg/kg, Group IV: CP and PGEE for 28 days. At the end of the treatment period, organ weight, body weight, epididymal sperm count, motility, morphology, SOD, catalase, GSH, ACP & testosterone level in the testis were evaluated. Results: The CP treated rats showed toxicity evidenced by decreased organ and body weight, decreased sperm quality and testosterone level also increase in MDA and decrease in antioxidants SOD, GSH indicating oxidative stress. In contrast PGEE co-treatment with CP resulted in significant restoration of the above mentioned parameters. Conclusion: These results indicate that PGEE attenuates CP induced testicular toxicity through its ROS scavenging activity.

  8. Punica granatum leave extract as green corrosion inhibitor for mild steel in Hydrochloric acid

    Abboud Y.

    2013-09-01

    Full Text Available Leave of Punica granatum extract (LPGE as green inhibitor for the corrosion of mild steel in 1M HCl solution was studied using weight-loss and potentiodynamic polarization measurements. The results obtained revealed that LPGE has fairly good inhibiting properties for mild steel corrosion in 1M HCl solution, with efficiency of around 94 % at a concentration of 1 g/l. The inhibition was of a mixed anodic–cathodic nature. The film which is formed over the metal surface was analysed by FT-IR spectroscopy. Further examination using X-ray diffraction confirms the role of LPGE as an effective corrosion inhibitor for mild steel in acid media.

  9. Studies on the Cytotoxic Activities of Punica granatum L. var. spinosa (Apple Punice) Extract on Prostate Cell Line by Induction of Apoptosis.

    Sineh Sepehr, Koushan; Baradaran, Behzad; Mazandarani, Masoumeh; Khori, Vahid; Shahneh, Fatemeh Zare

    2012-01-01

    The Punica granatum L. var. granatum (pomegranate) has been demonstrated to exert antitumor effects on various types of cancer cells. The present study aimed to evaluate the medicinal herbs Punica granatum L. var. spinosa (apple punice) that are native to Iran. This study was determined to test the possible cytotoxic activity and induction of apoptosis on human prostate cell lines. The effect of ethanol extracts of the herbs on the inhibition of cell proliferation was assessed by MTT colorimetric assay. PC3 cell lines treated with the extracts were analyzed for the induction of apoptosis by cell death detection (ELISA) and TUNEL assay. Dye exclusion analysis was performed for viability rate. Our results demonstrated that the Punica granatum L. var. spinosa extract dose dependently suppressed the proliferation of PC3 cells (IC(50)= 250.21 μg/mL) when compared with a chemotherapeutic anticancer drug (Toxol) (Vesper Pharmaceuticals) with increased nucleosome production from apoptotic cells. The Punica granatum L. var. spinosa extract attenuated the human prostate cell proliferation in vitro possibly by inducing apoptosis. The Punica granatum L. var. spinosa is likely to be valuable for the treatment of some forms of human prostate cell line. PMID:23320197

  10. Antibiotic activity of the extract of Punica granatum Linn. over bovine strains of Staphylococcus aureus Atividade antimicrobiana do extrato de Punica granatum Linn. em linhagens de Staphylococcus aureus de origem bovina

    Maria A. R. Silva; Jane S. Higino; Jozinete V. Pereira; José P. Siqueira-Júnior; Maria S.V. Pereira

    2008-01-01

    Human and veterinary medicines have not been so well succeeded in order to achieving their goals concerning the treatment of infections for long term caused by Staphylococcus aureus linked to resistance development against antibiotic agents. The antibiotic activity of the Punica granatum Linn. fresh fruit pericarp extract was evaluated by the agar diffusion method on 38 S. aureus strains, isolated from apparently healthy lactating cows in farms situated in counties of the semi-arid region of ...

  11. Antimicrobial Activity of Six Pomegranate (Punica granatum L. Varieties and Their Relation to Some of Their Pomological and Phytonutrient Characteristics

    Nurcan Erbil

    2009-05-01

    Full Text Available Arils from six pomegranate (Punica granatum L. varieties grown in the Mediterranean region of Turkey were tested for their antimicrobial properties by the agar diffusion and minimum inhibitory concentration (MIC methods against seven bacteria: (Bacillus megaterium DSM 32, Pseudomonas aeruginosa DSM 9027, Staphylococcus aureus Cowan 1, Corynebacterium xerosis UC 9165, Escherichia coli DM, Enterococcus faecalis A10, Micrococcus luteus LA 2971, and threefungi (Kluvyeromyces marxianus A230, Rhodotorula rubra MC12, Candida albicans ATCC 1023. It has been observed that the pomegranate aril extracts had antimicrobial effect on all microorganisms, giving inhibition zones ranging in size from 13 to 26 mm. The MIC values for active pomegranate extracts ranged between 30 and >90 µg/mL. The results obtained appeared to confirm the antimicrobial potential of the Punica granatum varieties.

  12. Antibacterial activity of crude ethanolic and fractionated extracts of Punica granatum Linn. fruit peels

    Éverton Silva Santos

    2015-10-01

    Full Text Available Currently it is clear the need to develop new antimicrobial seeking to solve problems such as antibiotic resistance, in this context medicinal plants has been using a prominent place, and knowledge of popular medicine shows itself to be a promising search tool. Peel of Punica granatum fruits are popularly used for the treatment of diarrhea, eye and upper airway inflammation, and in the external treatment of infectious sores. Thus, this study had the objective to evaluate the in vitro inhibitory effect of the crude ethanol extract of peels of P. granatum, three organic fractions and also fractions obtained by column chromatography, on reference microorganisms (Staphylococcus aureus, Escherichia coli, Salmonella typhimurium and Pseudomonas aeruginosa by disk diffusion method. The obtained results evidenced that the ethyl acetate and aqueous fractions facing S. aureus and E. coli showed significant antimicrobial activity, close to the antimicrobial gentamicin and penicillin, respectively. In its turn the crude ethanolic extract of P. granatum and aqueous fraction showed inhibitory effect similar to the antimicrobial tetracycline facing P. aeruginosa. It was observed an increase in the inhibition of the microorganisms with increasing extract volume (from 10 to 30 μL, being S. aureus and P. aeruginosa the most susceptible microorganisms. Differences in activity between the extracts and fractions can be partly explained by qualitative and quantitative variations in the secondary metabolites present in the extracts and fractions.Keywords: Pomegranade. Medicinal plants. Antimicrobial action. RESUMO Atualmente está clara a necessidade do desenvolvimento de novos antimicrobianos buscando resolver problemas como a resistência a antibióticos, neste contexto, as plantas medicinais vem utilizando um lugar de destaque, e os conhecimentos da medicina popular mostram-se uma ferramenta de busca promissora. Cascas dos frutos de Punica granatum s

  13. Wound healing activity of Malva sylvestris and Punica granatum in alloxan-induced diabetic rats.

    Pirbalouti, Abdollah Ghasemi; Azizi, Shahrzad; Koohpayeh, Abed; Hamedi, Behzad

    2010-01-01

    The flowers of Malva sylvestris Linn. (Malvaceae) and Punica granatum Linn. (Punicaceae) are important medicinal plants in Iranian traditional medicine (Unani) whose have been used as remedy against edema, bum, wound and for their carminative, antimicrobial and anti-inflammatory activities. The diethyl ether extract of M. sylvestris and P. granatum flowers were used to evaluate the wound healing activity at 200 mg/kg/day dose in alloxan-induced diabetic rats. Wounds were induced in Wister rats divided into six groups as following; Group I, normal rats were treated with simple ointment base. Group II, diabetic rats were treated with simple ointment base (control). Groups III and IV, diabetic rats were treated with simple ointment base containing of extracts (diabetic animals), Groups V, diabetic rats were treated with simple ointment base containing of mixed extracts (1:1), Group VI, diabetic rats received the standard drug (nitrofurazone). The efficacy of treatment was evaluated based on wound area relative and histopathological characteristics. The extract-treated diabetic animals showed significant reduction in the wound area when compared with control. Also, histological studies of the tissue obtained on days 9th and 18th from the extract-treated by extract of M. sylvestris showed increased well organized bands of collagen, more fibroblasts and few inflammatory cells. These findings demonstrate that extract of M. sylvestis effectively stimulates wound contraction as compared to control group and other groups. M. sylvestris accelerated wound healing in rats and thus supports its traditional use. PMID:20873419

  14. Bioactivity of Nonedible Parts of Punica granatum L.: A Potential Source of Functional Ingredients.

    Rummun, Nawraj; Somanah, Jhoti; Ramsaha, Srishti; Bahorun, Theeshan; Neergheen-Bhujun, Vidushi S

    2013-01-01

    Punica granatum L. has a long standing culinary and medicinal traditional use in Mauritius. This prompted a comparative study to determine the bioefficacy of the flower, peel, leaf, stem, and seed extracts of the Mauritian P. granatum. The flower and peel extracts resulting from organic solvent extraction exhibited strong antioxidant activities which correlated with the high levels of total phenolics, flavonoids, and proanthocyanidins. The peel extract had the most potent scavenging capacity reflected by high Trolox equivalent antioxidant capacity value (5206.01 ± 578.48 μmol/g air dry weight), very low IC50 values for hypochlorous acid (0.004 ± 0.001 mg air dry weight/mL), and hydroxyl radicals scavenging (0.111 ± 0.001 mg air dry weight/mL). Peel extracts also significantly inhibited S. mutans (P flower extract exhibited high ferric reducing, nitric oxide scavenging, and iron (II) ions chelation and significantly inhibited microsomal lipid peroxidation. Furthermore, it showed a dose-dependent inhibition of xanthine oxidase with an IC50 value of 0.058 ± 0.011 mg air dry weight/mL. This study showed that nonedible parts of cultivated pomegranates, that are generally discarded, are bioactive in multiassay systems thereby suggesting their potential use as natural prophylactics and in food applications. PMID:26904607

  15. Bioactivity of Nonedible Parts of Punica granatum L.: A Potential Source of Functional Ingredients

    Nawraj Rummun

    2013-01-01

    Full Text Available Punica granatum L. has a long standing culinary and medicinal traditional use in Mauritius. This prompted a comparative study to determine the bioefficacy of the flower, peel, leaf, stem, and seed extracts of the Mauritian P. granatum. The flower and peel extracts resulting from organic solvent extraction exhibited strong antioxidant activities which correlated with the high levels of total phenolics, flavonoids, and proanthocyanidins. The peel extract had the most potent scavenging capacity reflected by high Trolox equivalent antioxidant capacity value ( μmol/g air dry weight, very low IC50 values for hypochlorous acid ( mg air dry weight/mL, and hydroxyl radicals scavenging ( mg air dry weight/mL. Peel extracts also significantly inhibited S. mutans (, S. mitis (, and L. acidophilus ( growth compared to ciprofloxacin. The flower extract exhibited high ferric reducing, nitric oxide scavenging, and iron (II ions chelation and significantly inhibited microsomal lipid peroxidation. Furthermore, it showed a dose-dependent inhibition of xanthine oxidase with an IC50 value of  mg air dry weight/mL. This study showed that nonedible parts of cultivated pomegranates, that are generally discarded, are bioactive in multiassay systems thereby suggesting their potential use as natural prophylactics and in food applications.

  16. Rapid and Comprehensive Evaluation of (Poly)phenolic Compounds in Pomegranate (Punica granatum L.) Juice by UHPLC-MSn

    Daniele Del Rio; Alan Crozier; Renato Bruni; Chiara Dall'Asta; Gianni Galaverna; Cristina García-Viguera; Luca Calani; Pedro Mena

    2012-01-01

    The comprehensive identification of phenolic compounds in food and beverages is a crucial starting point for assessing their biological, nutritional, and technological properties. Pomegranate (Punica granatum L.) has been described as a rich source of (poly)phenolic components, with a broad array of different structures (phenolic acids, flavonoids, and hydrolyzable tannins) and a quick, high throughput, and accurate screening of its complete profile is still lacking. In the present work, a me...

  17. ARTEMISIA DRACUNCULUS, PUNICA GRANATUM AND BERBERIS VULGARIS INHIBITORY EFFECTS ON PLATELET ADHESION AND COAGULATION FACTORS IN RATS

    Dr. Razieh Yazdanparast et al

    2012-01-01

    Excessive platelet activity is one of the most important factors responsible for the incidence of cardiovascular diseases and, also, play important role in coagulation cascade. In this study, the comparative effects of methanol extracts of three herbs on adhesion of the activated platelets to fibrinogen coated plates and clotting factors were investigated. Artemisia dracunculus, Punica granatum and Brberis vulgaris are used as blood anti-coagulatory plants in Iranian folk medicine. Platelets ...

  18. Preventive Effect of Three Pomegranate (Punica granatum L.) Seeds Fractions on Cerulein-Induced Acute Pancreatitis in Mice

    Minaiyan, Mohsen; Zolfaghari, Behzd; Taheri, Diana; Gomarian, Mahdi

    2014-01-01

    Background: Acute pancreatitis (AP) refers to afflicted inflammation of pancreas with unfavorable adverse effects and developed multiple organ failures. Unfortunately, there is not a certain therapeutic method for this disease. Oxidative stress has a serious role in the pathogenesis of AP. Thus, decreasing of oxidative stress may prevent induction and progression of AP. Punica granatum L. has been extensively used in traditional medicine and possesses various active biological elements. Due t...

  19. Antimicrobial Activity of Six Pomegranate (Punica granatum L.) Varieties and Their Relation to Some of Their Pomological and Phytonutrient Characteristics

    Nurcan Erbil; Kenan S. Dayisoylu; Mustafa Ozgen; Ahmet D. Duman; Coskun Durgac

    2009-01-01

    Arils from six pomegranate (Punica granatum L.) varieties grown in the Mediterranean region of Turkey were tested for their antimicrobial properties by the agar diffusion and minimum inhibitory concentration (MIC) methods against seven bacteria: (Bacillus megaterium DSM 32, Pseudomonas aeruginosa DSM 9027, Staphylococcus aureus Cowan 1, Corynebacterium xerosis UC 9165, Escherichia coli DM, Enterococcus faecalis A10, Micrococcus luteus LA 2971), and threefungi (Kluvyeromyces marxianus A230, Rh...

  20. Effect of Punica granatum Linn. (flowers) on blood glucose level in normal and alloxan-induced diabetic rats.

    Jafri, M A; Aslam, M; Javed, K; Singh, S

    2000-06-01

    'Gulnar farsi', male abortive flowers of Punica granatum L., are used for the treatment of diabetes mellitus in Unani medicine. Oral administration of its aqueous-ethanolic (50%, v/v) extract led to significant blood glucose lowering effect in normal, glucose-fed hyperglycaemic and alloxan-induced diabetic rats. This effect of the extract was maximum at 400 mg/kg, b.w. PMID:10837992

  1. EFFECT OF AQUEOUS EXTRACT OF PUNICA GRANATUM FLOWER ON BIOMARKERS AND ECG CHANGES IN ISOPROTERENOL INDUCED MYOCARDIAL INFARCTION IN RATS

    Khatib N. A.; Patel Jignesh; Medi Swathi

    2011-01-01

    The present study was designed to investigate the effect of aqueous extract of Punica granatum flower (PG) against isoproterenol (ISO) induced myocardial infarction (MI) in rats by studying cardiac markers and electrocardiographic changes. MI was induced in rats by subcutaneous injection of ISO (150mg/kg b.w) at an interval of 24 hours for 2 days. ISO treated rats showed significant increase in cardiac markers such as Lactate dehydrogenase (LDH), Creatine kinase (CK-MB), Alanine aminotransfer...

  2. Two UGT84 Family Glycosyltransferases Catalyze a Critical Reaction of Hydrolyzable Tannin Biosynthesis in Pomegranate (Punica granatum)

    Ono, Nadia N.; Xiaoqiong Qin; Wilson, Alexander E.; Gang Li; Li Tian

    2016-01-01

    Hydrolyzable tannins (HTs) play important roles in plant herbivore deterrence and promotion of human health. A critical step in HT production is the formation of 1-O-galloyl-β-D-glucopyranoside (β-glucogallin, ester-linked gallic acid and glucose) by a UDP-glucosyltransferase (UGT) activity. We cloned and biochemically characterized four candidate UGTs from pomegranate (Punica granatum), of which only UGT84A23 and UGT84A24 exhibited β-glucogallin forming activities in enzyme assays. Although ...

  3. HPLC Evaluation of Phenolic Profile, Nutritive Content, and Antioxidant Capacity of Extracts Obtained from Punica granatum Fruit Peel

    Sushil Kumar Middha; Talambedu Usha; Veena Pande

    2013-01-01

    This study revealed polyphenolic content, nutritive content, antioxidant activity, and phenolic profile of methanol and aqueous extracts of Punica granatum peel extract. For this, extracts were screened for possible antioxidant activities by free radical scavenging activity (DPPH), hydrogen peroxide scavenging activity and ferric-reducing antioxidant power (FRAP) assays. The total phenolics and flavonoid recovered by methanolic (MPE) and the water extract (AQPE) were ranged from 185 ± 12.45 t...

  4. A hepatoprotective effect of an aqueous extract of pomegranate ( Punica granatum L. ) rind against acetaminop hen treated rats

    Enas A.M.Khalil

    2004-01-01

    In ancient times Egyptians regarded pomegranate fruit as a symbol of fertility due to the round shape and abundant seeds .They used it to heal many ailments. Pomegranate (El-Ruwmon ) that fruit mentioned in our holy Quran not less than three times, that paid attention for its importance. This investigation aims to evaluate the hepatoprotective activity of an aqueous rind powder extract of pomegranate (Punica granatum L.) in its human therapeutic dose against overdose acetaminophen. Pretreatme...

  5. Draft Genome Sequence of Phosphate-Solubilizing Bacterium Paraburkholderia tropica Strain P-31 Isolated from Pomegranate (Punica granatum) Rhizosphere.

    Kaur, Chandandeep; Selvakumar, Govindan; Ganeshamurthy, Arakalgud Nanjundiah

    2016-01-01

    We report the 8.9 Mb draft genome sequence of phosphate-solubilizing bacterium Paraburkholderia tropica strain P-31, isolated from pomegranate (Punica granatum) rhizosphere. The draft genome sequence of Paraburkholderia tropica strain P-31 consists of 8,881,246 bp with a G+C content of 64.7%, 8,039 protein-coding genes, and 49 RNAs. PMID:27540068

  6. Osteogenic potential of punica granatum through matrix mineralization, cell cycle progression and runx2 gene expression in primary rat osteoblasts

    Siddiqui, Sahabjada; Arshad, Mohammad

    2014-01-01

    Background Osteoporosis is one of the prevalent diseases in ageing populations. Due to side effects of many chemotherapeutic agents, there is always a need to search for herbal products to treat the disorder. Punica granatum (PG) represent a potent fruit-bearing medicinal herb which exerted valuable anti-osteoporotic activities. The present study was carried out to validate the in vitro osteogenic effects of the PG seed extract in primary calvarial osteoblast cultures harvested from neonatal ...

  7. Effects of rhaponticum carthamoides versus glycyrrhiza glabra and punica granatum extracts on metabolic syndrome signs in rats

    Dushkin, Michael; Khrapova, Marina; Kovshik, Gennadiy; Chasovskikh, Marina; Menshchikova, Elena; Trufakin, Valeriy; Shurlygina, Anna; Vereschagin, Evgeniy

    2014-01-01

    Background Rhaponticum cathamoides (RC) is an endemic wild Siberian herb with marked medicinal properties that are still poorly understood. The aim of this study is to investigate the therapeutic potential of RC extract (ERC) compared to the effects of Glycyrrhiza glabra (EGG) and Punica granatum extracts (EPG) in a rat model with high-fat diet-(HFD)-induced signs of metabolic syndrome; therefore, this study addresses a significant global public health problem. Methods Six-month-old male Wist...

  8. Antifungal efficacy of Punica granatum, Acacia nilotica, Cuminum cyminum and Foeniculum vulgare on Candida albicans: An in vitro study

    Pai Mithun

    2010-01-01

    Full Text Available Background: The establishment and maintenance of oral microbiota is related not only to interbacterial coaggregations but also to interactions of these bacteria with yeasts. Hence, it is important for agents used in the treatment of oral diseases to have antifungal properties for effective therapy. Objective: The main purpose of this study was to evaluate the in vitro antifungal efficacy of Punica granatum, Acacia nilotica, Cuminum cyminum and Foeniculum vulgare on Candida albicans. Materials and Methods: The pomegranate peel is separated, dried and powdered. Fennel, cumin and acacia bark obtained from the tree are powdered. Candida is inoculated at 37˚C and seeded on Sabourauds agar medium. Sterilized filter papers saturated with 30 μl of the extracts are placed on the seeded plates and inoculated at 24 and 48 h. Zones of inhibition on all four sides are measured around the filter paper with a vernier caliper. The experiments were repeated on four plates, with four samples of each extract on one plate for all of the extracts. Results: All the above-mentioned ingredients showed antifungal property, with Punica granatum showing the highest inhibition of Candida albicans with a mean zone of inhibition of 22 mm. P-values <0.05 were obtained for Punica granatum when compared with the other extracts. Conclusion: The results showed the potential use of these products as cheap and convenient adjuvants to pharmaceutical antifungal products.

  9. Evaluation of antidiabetic, hypolipedimic and antioxidant activity of hydroalcoholic extract of leaves and fruit peel of Punica granatum in male Wistar albino rats

    Salwe, Kartik J.; Sachdev, Devender O.; Bahurupi, Yogesh; Kumarappan, Manimekalai

    2015-01-01

    Background: We investigated anti-diabetic, hypolipedimic and antioxidant activity of hydroalcoholic extract from leaves and fruit peel of Punica granatum. Materials and Methods: Streptozotocin induced diabetic Wister rats were used in this study consisting of seven groups of six animals each. Groups (1) normal control, (2) diabetic control, (3) leaves extract 100 mg/kg b.w. of P. granatum, (4) leaves extract 200 mg/kg b.w. of P. granatum, (5) fruit peel extract 100 mg/kg b.w. of P. granatum, ...

  10. Inhibitory Effect of the Punica granatum Fruit Extract on Angiotensin-II Type I Receptor and Thromboxane B2 in Endothelial Cells Induced by Plasma from Preeclamptic Patients.

    Kusumawati, Widya; Keman, Kusnarman; Soeharto, Setyawati

    2016-01-01

    This study aims to evaluate whether the Punica granatum fruit extract modulates the Angiotensin-II Type I receptor (AT1-R) and thromboxane B2 level in endothelial cells induced by plasma from preeclamptic patients. Endothelial cells were obtained from human umbilical vascular endothelial cells. At confluence, endothelial cells were divided into five groups, which included endothelial cells exposed to 2% plasma from normal pregnancy (NP), endothelial cells exposed to 2% plasma from preeclamptic patients (PP), and endothelial cells exposed to PP in the presence of ethanolic extract of Punica granatum (PP + PG) at the following three doses: 14; 28; and 56 ppm. The expression of AT1-R was observed by immunohistochemistry technique, and thromboxane B2 level was done by immunoassay technique. Plasma from PP significantly increased AT1-R expression and thromboxane B2 levels compared to cells treated by normal pregnancy plasma. The increasing of AT1-R expression significantly (P Punica granatum extract. Moreover, the increasing of thromboxane B2 levels significantly (P Punica granatum extract. We further concluded that Punica granatum fruit protects and inhibits the sensitivity of endothelial cells to plasma from preeclamptic patients due to inhibition of AT1-R expression (56 ppm) and reduced thromboxane B2 levels (14 ppm). PMID:26989513

  11. Antidiabetic and antihyperlipidemic effects of ethanolic extract of leaves of Punica granatum in alloxan-induced non-insulin-dependent diabetes mellitus albino rats

    Swarnamoni Das; Sarajita Barman

    2012-01-01

    Objectives: Punica granatum L., (Family: Punicaceae) is used in Indian Unani medicine for treatment of diabetes mellitus. Therefore, the present study was done to evaluate the antidiabetic and antihyperlipidemic effects of ethanolic extract of leaves of P. granatum in alloxan-induced diabetic rats. Materials and Methods: Healthy Wistar albino rats (100-150 g) were divided into four groups of six animals each. Groups A and B received normal saline [(10 ml/kg/day/per oral (p.o.)]; group C ...

  12. Lipid Lowering Effect of Punica granatum L. Peel in High Lipid Diet Fed Male Rats

    Alireza Sadeghipour

    2014-01-01

    Full Text Available Many herbal medicines have been recommended for the treatment of dyslipidemia. The antilipidemic effect of hydroethanolic extract of pomegranate peel (Punica granatum L. was investigated in high lipid diet fed male rats. Intraperitoneally administration of pomegranate peel extract (50, 100, 200, and 300 mg/kg body weight for 23 days on the levels of serum cholesterol, triglycerides, LDL, HDL, alkaline phosphatase (AP, aspartate aminotransferase (AST, and alanine aminotransferase (ALT in high lipid diet fed male rats was evaluated. Treatment of pomegranate extract decreased body weight in treated rats, significantly. Administration of the plant extract significantly decreased serum total cholesterol, triglycerides, LDL-C, alkaline phosphatise, AST, and ALT levels, whereas it increased serum HDL-C in high lipid diet fed rats in comparison to saline control group. Also, histopathological study showed that treatment of pomegranate peel extract attenuates liver damage in high lipid diet fed rats in comparison to saline group. It is concluded that the plant should be considered as an excellent candidate for future studies on dyslipidemia.

  13. Protective Effect of Pomegranate (Punica Granatum), Juice in Rats Consuming Aspartame Peroxidation and Antioxidant Status

    Pomegranate (Punica granatum) has been traditionally used as medicine in many countries due to its high antioxidant activity, which has been related to beneficial health properties. Aspartame (ASP) is a widely used artificial sweetener that may be harmful at abuse levels. The objective of this study was to evaluate the potential efficacy of pomegranate juice (PJ) in protecting tissues from the possible damage induced by aspartame. ASP treated rats were given ASP, by gavages, at a dose of 250 mg/kg /day for 28 days. PJ-ASP treated rats were given PI at a dose of 1ml /kg body weight/day along with ASP. The data obtained indicate that ASP administration results in no significant change in the concentration of serum triglycerides (TG), total cholesterol (Tc), low-density lipoprotein (LDL-c), high density lipoprotein (HDL-c) and glucose while significant decreases have been recorded for insulin. The results demonstrated also that aspartame promotes lipid peroxidation and decreases the level of antioxidants superoxide dismutase (SOD) and catalase (Cat) activities and reduced glutathione (GSH) contents in serum and liver tissues. Significant elevations in the activities of serum aspartate transaminase (AST), serum alanine transaminase (AL T) and alkaline phosphatase (ALP) were also observed. Administration of PI in parallel with aspartame protected rats from aspartame-induced oxidative injury and ameliorated liver function

  14. Triterpenes as uncompetitive inhibitors of α-glucosidase from flowers of Punica granatum L.

    Salah El Dine, Riham; Ma, Qiong; Kandil, Zeinab A; El-Halawany, Ali M

    2014-01-01

    The α-glucosidase and maltase inhibitory effects of Punica granatum L. flowers (PGF) were investigated. The methanol extract (PGFMe), n-hexane extract (PGFH), chloroform extract (PGFC) and the remaining water fraction (PGFW) were assayed for their α-glucosidase and maltase inhibitory effects. PGFW showed potent α-glucosidase inhibition with IC₅₀ of 0.8 μg/mL followed by PGFMe (IC₅₀ of 4.0 μg/mL) then PGFC (IC₅₀ of 5.21 μg/mL) in comparison to acarbose (0.9 μM). Due to its selectivity towards α-glucosidase, PGFC was subjected to bioactivity-guided isolation of its main active constituents. Five known compounds (1-5) were identified as β-sitosterol (1), oleanolic acid (2), ursolic acid (3), p-coumaric acid (4) and apigenin (5). Ursolic and oleanolic acids showed potent α-glucosidase inhibition (IC₅₀ of 39.0 and 35.0 μM, respectively), while they did not show significant maltase inhibition. Kinetic study using the double Lineweaver-Burk plot revealed that ursolic acid uncompetitively inhibited α-glucosidase in comparison with acarbose as a competitive inhibitor. PMID:24956202

  15. Punica granatum peel extract protects against ionizing radiation-induced enteritis and leukocyte apoptosis in rats

    Radiation-induced enteritis is a well-recognized sequel of therapeutic irradiation. Therefore we examined the radioprotective properties of Punica granatum peel extract (PPE) on the oxidative damage in the ileum. Rats were exposed to a single whole-body X-ray irradiation of 800 cGy. Irradiated rats were pretreated orally with saline or PPE (50 mg/kg/day) for 10 days before irradiation and the following 10 days, while control rats received saline or PPE but no irradiation. Then plasma and ileum samples were obtained. Irradiation caused a decrease in glutathione and total antioxidant capacity, which was accompanied by increases in malondialdehyde levels, myeloperoxidase activity, collagen content of the tissue with a concomitant increase 8-hydroxy-2'-deoxyguanosine (an index of oxidative DNA damage). Similarly, pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) and lactate dehydrogenase were elevated in irradiated groups as compared to control. PPE treatment reversed all these biochemical indices, as well as histopathological alterations induced by irradiation. Furthermore, flow cytometric measurements revealed that leukocyte apoptosis and cell death were increased in irradiated animals, while PPE reversed these effects. PPE supplementation reduced oxidative damage in the ileal tissues, probably by a mechanism that is associated with the decreased production of reactive oxygen metabolites and enhancement of antioxidant mechanisms. Adjuvant therapy of PPE may have a potential to support a successful radiotherapy by protecting against radiation-induced enteritis. (author)

  16. Components Analysis of Punica granatum Linn. Seed%石榴籽成分分析

    杭志奇; 韩清波; 许景松

    2010-01-01

    [目的]对石榴(Punica granatum Linn.)籽主要成分进行分析.[方法]测定石榴籽水分、蛋白质、脂肪、灰分、黄酮类物质和单宁含量,并分析石榴籽油脂肪酸组成成分和干燥脱脂石榴籽蛋白质中氨基酸成分.[结果]石榴籽中蛋白质和脂肪含量相对较高,其含量分别为13.62%、20.29%,石榴籽油中含有8种不饱和脂肪酸和4种饱和脂肪酸,其中不饱和脂肪酸相对含量为92.22%,9,12,15-十八碳三烯酸占82.76%.石榴籽蛋白质中氨基酸组成较完全,其中必需氨基酸含量为31.98%.[结论]该研究可为石榴籽的进一步利用奠定理论基础.

  17. In Vitro Antifungal Activity of Various Persian Cultivars of Punica granatum L. Extracts Against Candida species

    Bassiri-Jahromi

    2015-08-01

    Full Text Available Background Resistance of Candida species to antifungal agents has potentially serious implications for management of infections. Candida species are now the fourth most common organisms isolated from hospitalized patients. Prevention and control of these infections will require new antimicrobial agents. Plant-derived antifungal agents have always been a source of novel therapeutics. Objectives The aim of this study was to investigate the antifungal effect of pomegranate peel and pulp extracts against Candida species. Materials and Methods Pomegranate pulp and peel were dried and powdered separately. The dried powders were extracted using a soxhlet extractor. The antifungal effect of pomegranate peel and pulp extracts were determined in vitro by using the minimum inhibitory concentration (MIC against five standard species, including Candida albicans (ATCC 10231, Candida parapsilosis (ATCC 22019, Candida tropicalis (ATCC 750, Candida glabrata (PTCC 5297 and Candida krusei (PTCC 5295. Results Maximum inhibitions were attributed to peel extract of the pomegranate cultivar against Candida species. The greatest antifungal inhibition among the eight different cultivars was observed for sour malas, sour white peel and sour summer extracts respectively, against the five Candida strains. The antifungal activity of pulp extracts against Candida species was somewhat negative. Conclusions Our work suggested that pomegranate (Punica granatum L. peel has potential antifungal activity against Candidiasis, and it is an attractive option for the development of new management strategies for candidiasis.

  18. Efeito antibacteriano e antiaderente in vitro do extrato da Punica granatum Linn. sobre microrganismos do biofilme dental In vitro antibacterial and antiadherence effect of the extract of the Punica granatum Linn. upon dental biofilm microrganisms

    Jozinete V. Pereira; Maria S. Vieira Pereira; Fábio C. Sampaio; Maria C. Correia Sampaio; Pollianna M. Alves; Cristina R. Ferreira de Araújo; Jane S. Higino

    2006-01-01

    Nesta pesquisa foi avaliada a atividade antimicrobiana e a capacidade de inibição da síntese de glucano in vitro do extrato da casca do fruto da romã (Punica granatum Linn.) sobre linhagens bacterianas de Streptococcus mitis, Streptococcus mutans, Streptococcus sanguis, Streptococcus sobrinus e Lactobacillus casei. Os ensaios foram realizados pelas técnicas de ágar-difusão em placas para determinação da Concentração Inibitória Mínima (CIM) e técnica dos tubos inclinados para determinação da C...

  19. Comparison of antihyperlipidaemic activity of eugenia jambolana fruit with punica granatum fruit in diet induced hyperlipidaemic rats

    Objective: To compare the antihyperlipidemic effects of Eugenia Jambolana fruit pulp with Punica Granatum fruit in diet induced hyperlipidaemic rats at the same dose level. Methods: An experimental randomized control study was conducted on seventy five male albino rats over a period of 14 weeks in University of Health Sciences Lahore. They were divided into five groups labelled A, B, C, D and E with fifteen rats in each group. Group A was kept as normal control, groups B, C, D and E were given hyperlipidaemic diet for six weeks. In group B no further intervention was done, group C and D were given ethanolic extract of Eugenia Jambolana and Punica Granatum respectively for eight weeks. Group E was given combination of both for same duration. Serum total cholesterol (TC), high density lipoprotein cholesterol (HDL-c), lowdensity lipoprotein cholesterol (LDL-c) and triglycerides (TG) were measured at zero, six and fourteen weeks. Results: At fourteenth week significant reductions in TC, LDL-c and TG and a rise in HDL-c was observed in interventional groups C, D and E as compared to experimental hyperlipidaemic control group B (p 0.57, p > 0.22, p > 0.56, p > 0.76, respectively. On sixth week, there was no significant difference between groups B, C, D and E (p > 0.05). However, 15 rats of group A had significant lower levels of cholesterol, high density lipoproteins, low density lipoproteins and triglycerides when compared to 60 rats of groups B, C, D and E (p<0.05). Conclusion: In male albino rats combination of ethanolic extracts of Eugenia Jambolana and Punica Granatum fruit pulps was most effective in lowering serum total cholesterol and triglycerides while decrease in low density lipoprotein cholesterol and rise in high density lipoprotein cholesterol was same as the extracts given alone. (author)

  20. Amelioration of radiation induced decrease in activity of catalase and superoxide dismutase in mouse liver by Punica granatum

    Ionizing radiation generates reactive oxygen species (ROS) in irradiated tissue. Cells of liver have their own defence system, the antioxidant system to deactivate ROS. Antioxidant system includes enzymatic and non-enzymatic components. Liver is rich in endogenous antioxidants and related enzymes. Catalase and Superoxide dismutase (SOD) are powerful antioxidant enzymes. In the present study Punica granatum fruit rind Ethanol extract (PGFRE) was tested against 60Co gamma radiation induced alteration in Swiss albino mouse. Healthy adult (25±2) Swiss albino mouse were selected and divided into four groups. The first group was sham irradiated. The second group was irradiated with 8 Gy 60Co gamma radiation only and served as control. The third group was administered with Ethanol extract of Punica granatum fruit rind one hour before irradiation at the dose rate of 10 mg/kg body weight orally. Animals were exposed to 8 Gy 60Co gamma radiation. Fourth group was administered with Ethanol extract of Punica granatum fruit rind at the dose rate of 10 mg/kg body weight. Mice were sacrificed at various post irradiation intervals and liver was removed, weighed and analysed biochemically for Catalase and SOD activity. Catalase and SOD activity decreased up till 7th post irradiation day in 8 Gy irradiated group than normal. In PGFRE pretreated irradiated group catalase and SOD activity were higher than the corresponding control group at all the intervals. These results indicate that PGFRE extract protects damage to the catalase and SOD activity in liver of Swiss albino mouse against lethal dose of gamma radiation. (author)

  1. Inhibitory Effects of Two Varieties of Tunisian Pomegranate (Punica granatum L.) Extracts on Gastrointestinal Transit in Rat.

    Souli, Abdelaziz; Sebai, Hichem; Rtibi, Kais; Chehimi, Latifa; Sakly, Mohsen; Amri, Mohamed; El-Benna, Jamel; Marzouki, Lamjed

    2015-09-01

    The present study was undertaken to determine whether total and methanol juice extracts of two Tunisian Pomegranate (Punica granatum L.) varieties (Garsi and Gabsi) protect against diarrhea as well as their effects on gastrointestinal transit (GIT) in healthy rats. In this respect, male Wistar rats were used and divided into control- and pomegranate-treated groups. The antidiarrheal activity was evaluated using the castor oil-induced diarrhea method and the GIT was assessed using charcoal meal. Our results showed that total and methanol P. granatum juice extracts produced a significant dose-dependent protection against castor oil-induced diarrhea. Pomegranate extracts and juice also decreased the GIT significantly and dose dependently. Importantly, the Garsi variety appeared to be more effective than the Gabsi variety on these two parameters. These findings suggest that pomegranate extracts have a potent antidiarrheal property in rats confirming their efficiency in the Tunisian traditional medicine. PMID:25775227

  2. New Development on Pharmacological Effects ofPunica Granatum L.%石榴药理研究新进展

    刘宇; 蔡霞; 曾勇; 王平; 孟宪丽; 张艺

    2015-01-01

    本文主要对近年来国内外石榴的药理研究进展进行综述.石榴药用历史悠久,不同部位在中医药与民族医药中应用广泛,其化学成分以鞣质类、黄酮类、生物碱和有机酸化合物为主,具有明显的药理活性.近年来,有关石榴的抗菌抗病毒、降血糖、抗肿瘤、调血脂及抗氧化、免疫调节等药理研究进展迅速.通过整理归纳并科学的分析石榴的功效及其新的药理学研究成果,以期能对石榴的研究、临床应用及相关产品的开发利用提供依据.%This article reviewed pharmacological study development of Punica granatum L. both at home and abroad in recent years. P. granatum has a long medical usage history. Different parts of P. granatum have wide applications in both traditional Chinese medicine (TCM) and other ethnic medicine. Its main chemical compositions were tannins, flavonoids, alkaloids and organic acids. It has obvious pharmacological activities. In recent years, the pharmacological research progress on P. granatum in terms of antibacterial, antiviral, hypoglycemic, antitumor, adjusting blood lipids, antioxidant, and immunoregulation has rapid development. The scientific collection and analysis on effects and new pharmacological research results of P. granatum are hoped to provide references for the study, clinical application, development and utilization of P. granatum and its products.

  3. Antispasmodic Effects of Aqueous and Hydroalcoholic Punica granatum Flower Extracts on the Uterus of Non-pregnant Rats

    Ahangarpour, Akram; Heidari, Razieh; Abdolahzadeh, Mahsa; Oroojan, Ali Akbar

    2012-01-01

    Background Punica granatum Linn. (PG) is native to the Mediterranean region. Its flower exhibited antioxidant activity. The present study attempt to investigate the effect of these extract on uterine contraction and its possible mechanism(s). Methods Thirty five female Wistar rats (200–300 g) at estrous phases of cycle was examined in this study; pieces of virgin adult rat uterus (1.5 cm) were suspended in an organ bath containing 10 ml of De Jalon solution at 29 °C. Tissue contractility was ...

  4. Amelioration of Diabetes and Painful Diabetic Neuropathy by Punica granatum L. Extract and Its Spray Dried Biopolymeric Dispersions

    Raafat, K.; Samy, W

    2014-01-01

    Aims. To evaluate the effect of Punica granatum (Pg) rind extract and its spray dried biopolymeric dispersions with casein (F1) or chitosan (F2) against Diabetes mellitus (DM) and diabetic neuropathy (DN). Methods. We measured the acute (6 h) and subacute (8 days) effect of various doses of Pg, F1, and F2 and the active compounds on alloxan-induced DM mouse model. We evaluated DN utilizing latency tests for longer period of time (8 weeks). In addition, the in vivo antioxidant activity was ass...

  5. Antibiotic activity of the extract of Punica granatum Linn. over bovine strains of Staphylococcus aureus Atividade antimicrobiana do extrato de Punica granatum Linn. em linhagens de Staphylococcus aureus de origem bovina

    Maria A. R. Silva

    2008-06-01

    Full Text Available Human and veterinary medicines have not been so well succeeded in order to achieving their goals concerning the treatment of infections for long term caused by Staphylococcus aureus linked to resistance development against antibiotic agents. The antibiotic activity of the Punica granatum Linn. fresh fruit pericarp extract was evaluated by the agar diffusion method on 38 S. aureus strains, isolated from apparently healthy lactating cows in farms situated in counties of the semi-arid region of the State of Paraíba, Brazil to determine the minimum inhibitory concentration (MIC. Twenty-two of the thirty-eight strains are penicillin-resistant (PRSA. The extract of P. granatum presented potential antibiotic action over all the assayed strains, forming 10 to 36 mm diameter inhibition zones. This paper's results claim the effectiveness of the extract of P. granatum as a potential antibacterial agent on S. aureus, and display the significance of evaluating new substances with antimicrobial potential, which can contribute to alternative therapeutics for veterinary and medicine.A medicina humana e veterinária tem falhado no tratamento de infecções em longo prazo causadas por Staphylococcus aureus associadas ao desenvolvimento de resistência a agentes antimicrobianos. A atividade antimicrobiana do extrato da casca do fruto de Punica granatum Linn. foi avaliada sobre 38 linhagens de S. aureus de origem bovina para a determinação da concentração inibitória mínima (CIM. Das 38 linhagens ensaiadas, 22 são resistentes à penicilina.(PRSA. O extrato de P. granatum apresentou potencial ação antimicrobiana sobre todas as linhagens ensaiadas formando halos de inibição variando de 10 a 36 mm de diâmetro. Os resultados deste estudo confirmam a eficácia do extrato de P. granatum como potencial agente antibacteriano sobre S. aureus e demonstram a importância de se avaliar novas substâncias com potencial antimicrobiano, o que pode contribuir para a

  6. Antioxidant Activity of Some Extracts from GAMMA Irradiated Pomegranate (Punica granatum L.) Peel and Seed

    The present study aims to evaluate the antioxidant activity of pomegranate (Punica granatum L.) peel and seed (obtained as waste from juice extraction) using different solvents as diethyl ether, ethyl acetate, ethanol 50 ⁒, ethanol 80⁒: methanol 50⁒, methanol 80⁒ and distilled water. The measurements of the antioxidant activity of all extracts were carried out using a radical scavenging activity against 2,2',-diphenyl-1-picrylhydrazyl (DPPH), β-Carotene⁒linoleic acid bleaching and ferric reducing antioxidant power (FRAP). Moreover, the effect of gamma irradiation at dose levels of 3, 6 and 9 kGy on, antioxidant activity of the best pomegranate peel and seed samples that possessed highest antioxidant activity was investigated. Results showed that ethanolic 50⁒ peel extract had a higher total phenolic contents (TPC) and total flavonoid contents (TFC) in both peel and seed, (9323.17 mg gallic acid equivalent (GAE) 100 g-1, 2998.05 mg quercetin equivalent (QE) 100 g-1 and 352.09 mg GAE 100 g-1,106.78 mg QE 100 g-1 dry weight (DW), respectively than other extracts. Ethanolic 50⁒ extracts showed higher antioxidant activity than other peel and seed extracts. In addition, ethanolic 50⁒ extract of irradiated pomegranate peel and seed at dose level of 6 kGy extract had higher TPC, TFC and antioxidant activity compared to other doses. Thus, ethanolic 50⁒ extract of irradiated pomegranate peel and seed at 6 kGy may be considered as a good source of natural compounds with-antioxidant activity which could be suitable as potential ingredient for food products.

  7. Lipid composition of seed oils of different pomegranate (Punica granatum L. cultivars from Spain

    Luana Fernandes

    2015-04-01

    Full Text Available Pomegranate (Punica granatum L. is an ancient fruit tree traditionally cultivated in the Near and Middle East. Presently, its most important growing regions include Afghanistan, Iran, Israel, USA, Italy and Spain, the latter country the largest European exporter. The pomegranate fruit can be divided into several anatomical compartments: outside peel, inside peel, and arils (pulp and seeds, the last part being usually used for consumption as is or for juice, jams and jellies production. Even though pomegranate seeds are an industrial by-product, recent reports have highlighted their potential use as a source of oil with beneficial chemical attributes. Therefore, the main objective of the present work was to characterize the seed oil of nine European pomegranate varieties, collected in Spain, for their fatty acid and vitamin E compositions. All seed lipid fractions consisted mainly of punicic acid (c9,t11,c13 C-18:3, ranging between 77.3% and 83.6% of total fatty acids, followed by small amounts of linoleic acid (C18:2n6, oleic acid (C18:1n9 and palmitic acid (C16:0. Regarding vitamin E composition, α-, γ-, δ-tocopherols were found in all pomegranate seed oils, but mainly γ-tocopherol, with total tocopherols ranging from 174.5 to 627.3 mg/100g oil.The richness of these pomegranate varieties seed oils in punicic acid, a conjugated linolenic acid with interesting anti-carcinogenic activity, and the elevated amount of tocopherols on the extracted lipids, of technological and nutritional relevance, make this by-product interesting for further exploitation.

  8. COMPORTAMENTO DE SEMENTES DE ROMÃ (Punica granatum L. SUBMETIDAS À FERMENTAÇÃO E SECAGEM

    LOPES KILSON PINHEIRO

    2001-01-01

    Full Text Available Sementes de romã (Punica granatum L. foram submetidas a diferentes períodos de fermentação (0; 24; 48; 72 e 96 horas, com e sem secagem. Avaliaram-se suas qualidades física e fisiológica, através das determinações do grau de umidade, peso de 100 sementes, porcentagem de emergência e velocidade de emergência de plântulas (IVE. O delineamento experimental adotado foi inteiramente casualizado, em esquema fatorial, onde os cinco períodos de fermentação frente às duas condições (com e sem secagem constituíram os tratamentos. Sementes submetidas ao maior período de fermentação apresentaram sarcotestas mais degradadas, favorecendo, conseqüentemente, uma melhor absorção de água, resultando numa porcentagem de emergência de 55% e IVE de 0,38, em 72 horas de fermentação. A secagem das sementes após fermentação promoveu uma menor porcentagem de emergência e IVE, apesar de se verificar um aumento de ambos, quando as sementes permaneceram em fermentação por um maior período. O comportamento verificado nas sementes de romã frente aos tratamentos empregados parece estar associado a fenômenos de dormência ou sensibilidade à secagem, característica esta, de sementes recalcitrantes.

  9. ARTEMISIA DRACUNCULUS, PUNICA GRANATUM AND BERBERIS VULGARIS INHIBITORY EFFECTS ON PLATELET ADHESION AND COAGULATION FACTORS IN RATS

    Dr. Razieh Yazdanparast et al

    2012-10-01

    Full Text Available Excessive platelet activity is one of the most important factors responsible for the incidence of cardiovascular diseases and, also, play important role in coagulation cascade. In this study, the comparative effects of methanol extracts of three herbs on adhesion of the activated platelets to fibrinogen coated plates and clotting factors were investigated. Artemisia dracunculus, Punica granatum and Brberis vulgaris are used as blood anti-coagulatory plants in Iranian folk medicine. Platelets were prepared and incubated with different concentration of the test samples (50-200 μg/ml for 60 min. The treated and un-treated platelets were then activated with thrombin (0.25U/ml and their adhesions to fibrinogen coated plates were investigated. Based on obtained data, the methanol extract of Artemisia dracunculus, Brberis vulgaris and Punica granatum at a concentration of 200 μg /ml reduced platelet adhesion to coated wells by 35%, 25% and 20%, respectively. In addition, the effects of the crude extracts of each plant on atherogenic lipoproteins were also examined. The results indicated that the LDL and cholesterol concentration were dramatically reduced by 56% and 36%, respectively, by B. vulgaris. This result provided the scientific basis for the traditional use of A.dracunculus and B. vulgaris in treatment of cardiovascular related disorders.

  10. Evaluation of Protective Effect of Hydro-alcoholic Extract of Fruit Peels of Punica granatum Linn against Ulcerative Colitis in Rats

    Ashar Kalangottil

    2014-07-01

    Full Text Available The study was designed to evaluate the protective effect of hydro-alcoholic extract of fruits peels of Punica granatum Linn. against acetic acid and trinitrobenzene sulfonic acid (TNBS induced ulcerative colitis in rats. Acute oral toxicity study was performed to find out the test dose according to OECD guidelines 425 and hydro-alcoholic extract of P. granatum fruit peel (PGPE was found to be safe at a dose of 2000 mg/kg body weight. The animals pretreated with hydro-alcoholic extract of P. granatum fruit peel (200 mg/kg and 400 mg/kg respectively significantly restored the altered hematological, biochemical parameters to normal levels when compared with control. The protective effect of P. granatum peel extract was comparable to standard sulfasalazine. The findings of the present study revealed that the hydro-alcoholic extract of P. granatum fruit peel possessed a dose dependent significant inhibitory activity against ulcerative colitis. The results obtained established the efficacy of the Punica granatum fruit peel against inflammatory bowel diseases possibly by its anti-inflammatory and antioxidant properties.

  11. Effect of Punica granatum L. Flower Water Extract on Five Common Oral Bacteria and Bacterial Biofilm Formation on Orthodontic Wire.

    Elahe Vahid Dastjerdi

    2014-12-01

    Full Text Available Use of herbal extracts and essences as natural antibacterial compounds has become increasingly popular for the control of oral infectious diseases. Therefore, finding natural antimicrobial products with the lowest side effects seems necessary. The present study sought to assess the effect of Punica granatum L. water extract on five oral bacteria and bacterial biofilm formation on orthodontic wire.Antibacterial property of P. granatum L. water extract was primarily evaluated in brain heart infusion agar medium using well-plate method. The minimum inhibitory concentration and minimum bactericidal concentration were determined by macro-dilution method. The inhibitory effect on orthodontic wire bacterial biofilm formation was evaluated using viable cell count in biofilm medium. At the final phase, samples were fixed and analyzed by Scanning Electron Microscopy.The growth inhibition zone diameter was proportional to the extract concentration. The water extract demonstrated the maximum antibacterial effect on Streptococcus sanguinis ATCC 10556 with a minimum inhibitory concentration of 6.25 mg/ml and maximum bactericidal effect on S. sanguinis ATCC 10556 and S. sobrinus ATCC 27607 with minimum bactericidal concentration of 25 mg/ml. The water extract decreased bacterial biofilm formation by S. sanguinis, S. sobrinus, S. salivarius, S. mutans ATCC 35608 and E. faecalis CIP 55142 by 93.7-100%, 40.6-99.9%, 85.2-86.5%, 66.4-84.4% and 35.5-56.3% respectively.Punica granatum L. water extract had significant antibacterial properties against 5 oral bacteria and prevented orthodontic wire bacterial biofilm formation. However, further investigations are required to generalize these results to the clinical setting.

  12. Evaluation of Protective Effect of Hydro-alcoholic Extract of Fruit Peels of Punica granatum Linn against Ulcerative Colitis in Rats

    Ashar Kalangottil; Karunakar Hegde; Naseeb K M

    2014-01-01

    The study was designed to evaluate the protective effect of hydro-alcoholic extract of fruits peels of Punica granatum Linn. against acetic acid and trinitrobenzene sulfonic acid (TNBS) induced ulcerative colitis in rats. Acute oral toxicity study was performed to find out the test dose according to OECD guidelines 425 and hydro-alcoholic extract of P. granatum fruit peel (PGPE) was found to be safe at a dose of 2000 mg/kg body weight. The animals pretreated with hydro-alcoholic extract of P....

  13. Antidiabetic effect of an aqueous extract of Pomegranate (Punica granatum L. peels in normal and alloxan diabetic rats

    Enas A. M. Khalil

    2004-09-01

    Full Text Available Hypoglycaemic drugs are either too expensive or have undesirable side effects including hematological, coma and disturbances of liver and kidney. Limiting of diabetes without any side effects is still a challenge to the medical system. This leads to exert effort to search for effective, safer and less cost antidiabetic plants. This investigation aims to evaluate the role of Punica granatum powder peels extract in its human therapeutic dose on beta cell numbers blood glucose and plasma insulin levels in normal and alloxan diabetic rats for 4-weeks of treatment. The treatment revealed that pomegranate aqueous extract significant decreased blood glucose and increased insulin levels in normal and diabetic treated rats. Pancreas showed increased number of beta cells in normal and treated diabetic rats. In conclusion pomegranate peel aqueous extract can reduce blood sugar through regeneration of ß cells.

  14. In vitro assessment of the antimicrobial effects of pomegranate (Punica granatum L. peel decoction on saliva samples

    Solon José de Oliveira Leite

    2014-01-01

    Full Text Available Several products have been developed to eliminate or reduce potential pathogenic microorganisms of the oral microbiome. The continuous use of these synthetic products can result in side effects such as vomiting, diarrhea, darkening of the teeth and the induction of microbial resistance. Pomegranate (Punica granatum peel decoction was tested to assess its antimicrobial activity. In vitro analysis showed the decoction had antimicrobial activity against strains of Pseudomonas aeruginosa and Candida albicans, but none was detected against Enterococcus faecalis. When tested on saliva samples from children, the decoction showed great potential in reducing the load of microorganisms, the inhibition haloes produced with saliva samples being similar to those of the antimicrobial control (0.12% chlorhexidine. The pomegranate peel decoction in water could thus provide a promising source for developing solutions for use against oral diseases.

  15. EFFECT OF AQUEOUS EXTRACT OF PUNICA GRANATUM FLOWER ON BIOMARKERS AND ECG CHANGES IN ISOPROTERENOL INDUCED MYOCARDIAL INFARCTION IN RATS

    Khatib N.A

    2011-03-01

    Full Text Available The present study was designed to investigate the effect of aqueous extract of Punica granatum flower (PG against isoproterenol (ISO induced myocardial infarction (MI in rats by studying cardiac markers and electrocardiographic changes. MI was induced in rats by subcutaneous injection of ISO (150mg/kg b.w at an interval of 24 hours for 2 days. ISO treated rats showed significant increase in cardiac markers such as Lactate dehydrogenase (LDH, Creatine kinase (CK-MB, Alanine aminotransferase (ALT, Aspartate aminotransferase (AST in serum. Altered ECG pattern with ST segment elevation, which conforms the MI. Oral pretreatment with PG at 400mg/kg and Vitamin E (100mg/kg as a standard daily for a period of 28 days showed significant decrease in serum cardiac marker enzymes level and ECG alterations. Thus the result suggests that PG has cardioprotective effect in ISO induced myocardial infracted rats.

  16. Mucoadhesive polyethylenimine-dextran sulfate nanoparticles containing Punica granatum peel extract as a novel sustained-release antimicrobial.

    Tiyaboonchai, Waree; Rodleang, Ingdao; Ounaroon, Anan

    2015-06-01

    Mucoadhesive polyethylenimine-dextran sulfate nanoparticles (PDNPs) were developed for local oral mucosa delivery. Punica granatum peel extract (PGE) was loaded into PDNPs for oral malodor reduction and caries prevention. PDNPs were constructed using the polyelectrolyte complexation technique employing oppositely charged polymers polyethylenimine (PEI) and dextran sulfate (DS), with PEG 400 as a stabilizer. Under optimal conditions, spherical particles of ∼ 500 nm with a zeta potential of ∼+28 mV were produced. Up to 98%, drug entrapment efficiency was observed. The mass ratio of PEI:DS played a significant role in controlling particle size and entrapment efficacy. PDNPs shown to be a good mucoadhesive drug delivery system as confirmed by ex vivo wash off test. In vitro dissolution studies revealed that PGE-loaded PDNPs manifested a prolong release characteristic with a burst release within 5 min. In addition, they remained effectively against oral bacteria. PMID:24438035

  17. Influence of Punica granatum L. on region specific responses in rat brain during Alloxan-Induced diabetes

    Sushil Kumar Middha; Talambedu Usha; Tekupalli RaviKiran

    2012-01-01

    Objective: The present study was carried out to investigate the effects of Punica granatum peel methanolic extract (PGPE) on cerebral cortex (CC) and Hippocampus (HC) brain antioxidant defense system and markers of lipid and protein oxidation in alloxan induced diabetic rats.Methods:Oral administration of PGPE (75 and 150 mg of kg body weight) for 45 days resulted in significant reduction in blood glucose levels. Results: Supplementation of diabetic rats with PGPE showed increased activities of SOD and GPx with concomitant decrease in MDA and PC content. Region-specific changes were more evident in the HC when compared to CC. Conclusions: The present study indicated that PGPE can ameliorate brain oxidative stress in alloxan induced diabetic rats by up regulating antioxidant defense mechanism by attenuating lipid and protein oxidation. PGPE thus may be used as a potential therapeutic agent in preventing diabetic complications in the brain.

  18. The Investigation for Antifungal Effect of Medicinal Plants, Combination Infusion of Piper Bettle Leaves, Punica Granatum Fructus Cortec, Curcuma Domestica Rhizome

    Berna Elya

    2002-12-01

    Full Text Available The tested demartophyta used Candida albicans. This research using dilution method to determine of minimum inhibitory concentration (MIC and difusion method to determine zone inhibition around of disc. The results of determination of MIC showed that combination infusion Piper bettle leaves with Punica granatum Fructus Cortex against C. albicans respectively, 31,2 mg/ml : 7,8 mg/ml; 15,6 mg/ml : 15,6 mg/ml and 7,8 mg/ml : 31,2 mg/ml. The determination for zone inhibition from 3 concentration of infusion of Piper bettle leaves with 3 concentration of Punica granatum Fructus Cortex with 9 combination against C. albicans showed that combination of two infusion is the larger than the single infusion.

  19. Inhibitory Effect of the Punica granatum Fruit Extract on Angiotensin-II Type I Receptor and Thromboxane B2 in Endothelial Cells Induced by Plasma from Preeclamptic Patients

    Widya Kusumawati; Kusnarman Keman; Setyawati Soeharto

    2016-01-01

    This study aims to evaluate whether the Punica granatum fruit extract modulates the Angiotensin-II Type I receptor (AT1-R) and thromboxane B2 level in endothelial cells induced by plasma from preeclamptic patients. Endothelial cells were obtained from human umbilical vascular endothelial cells. At confluence, endothelial cells were divided into five groups, which included endothelial cells exposed to 2% plasma from normal pregnancy (NP), endothelial cells exposed to 2% plasma from preeclampti...

  20. Parasiticidal and brine shrimp cytotoxicity potential of crude methanolic extract of rind of Punica granatum Linn against round worms and tape worms.

    Ali, Niaz; Jamil, Ayesha; Shah, Syed Wadood Ali; Shah, Ismail; Ahmed, Ghayour; Junaid, Muhammad; Ahmed, Zahoor

    2015-05-01

    Rind of Punica granatum is traditionally used for anthelmintic purposes. The current work describes the possible anthelmintic activity of crude methanolic extract of Punica granatum (Pg. Cr) against round worms (Ascaridia galli) and the tape worms (Raillietina spiralis). Brine shrimp cytotoxicity is also performed. Brine shrimp cytotoxic activity was tested using different concentrations (1000 μg/mL, 100 μg/mL and 10 μg/mL) of Pg.Cr. In vitro anthelmintic activity of Pg. Cr was determined against the parasites using albendazole and piperazine citrate as standard anthelmintic drugs in concentration 10 mg/ml. LC50 value for Brine shrimp cytotoxicity was 189.44 ±28 μg/mL. In test concentration of 40mg/ml of the Pg. Cr, Raillietina spiralis was paralyzed in 23 minutes. However, for parasiticidal activity (death of the parasite), it took less time (40 minutes) as compared to standard Albendazole. Time taken for death of the parasite Raillietina spiralis, in concentration 40 mg /ml, is 40 min. While standard drugs took more time to kill the Raillietina spiralis. Pg. Cr took 19 minutes to paralyze the Ascaridia galli at concentration 40 mg/ml whereas; it took 48 minutes for to kill the parasite Ascaridia galli. The current work confirms the traditional use of rind of Punica granatum as anthelmintic against Raillietina spiralis and Ascaridia galli. Results of brine shrimp cytotoxicity assay warrant for the isolation of cytotoxic compounds. List of abbreviation- Pg. Cr = Crude methanolic extract of Punica granatum. PMID:26004729

  1. Antifungal activity of the ethanolic extracts of Punica granatum L. and evaluation of the morphological and structural modifications of its compounds upon the cells of Candida spp.

    Paula Cristina Anibal; Iza Teixeira Alves Peixoto; Mary Ann Foglio; José Francisco Höfling

    2013-01-01

    Ethanolic crude extracts prepared from the arils and seeds, pericarp, peels and from the whole fruit of Punica granatum, known as pomegranate, had their antifungal activity tested against Candida spp. The ethanolic crude extracts were analyzed by Mass Spectrometry and yielded many compounds such as punicalagin and galladydilacton. The extracts from the pericarp and peel showed activity against Candida spp., with MICs of 125 μg/mL. The effect of pericarp and peel extracts upon the morphologica...

  2. Protective Effects of Combined Selenium and Punica granatum Treatment on Some Inflammatory and Oxidative Stress Markers in Arsenic-Induced Hepatotoxicity in Rats.

    Shafik, Noha M; El Batsh, Maha M

    2016-01-01

    Oxidative stress is one of the major mechanisms implicated in inorganic arsenic poisoning. Punica granatum is known by its free radical scavenging properties. The aim of this study was to evaluate the protective role of combined selenium and P. granatum against arsenic-induced liver injury. Seventy-five female albino rats were divided into five groups (of 15 rats each). Toxicity was induced by oral sodium arsenite (5.5 mg/kg body weight (bw) daily) (group ІІ). Treatment of arsenic-intoxicated rats was induced by daily oral administration of sodium selenite (3 mg/kg bw) (group ІІІ), 100 mg of P. granatum ethanol extract per kilogram body weight dissolved in 300 mL distilled water in three divided doses (100 mL of this suspension every 8 h) (group IV), and combined daily oral treatment with both selenite and P. granatum ethanol extract (group V). After 3 weeks, serum and liver tissues were obtained from the decapitated rats for different estimations. Hepatotoxicity was demonstrated by significant elevation in liver weights and activities of liver enzymes, alanine aminotransferase (ALT) and aspartate aminotransferase (AST), and decrease in serum total proteins and albumin (p granatum and selenium. It was concluded that combined P. granatum and selenium treatment had a synergistic hepatoprotective effect against arsenic toxicity through activation of Nrf2 anti-oxidant pathway. PMID:26085057

  3. Rapid and Comprehensive Evaluation of (Polyphenolic Compounds in Pomegranate (Punica granatum L. Juice by UHPLC-MSn

    Daniele Del Rio

    2012-12-01

    Full Text Available The comprehensive identification of phenolic compounds in food and beverages is a crucial starting point for assessing their biological, nutritional, and technological properties. Pomegranate (Punica granatum L. has been described as a rich source of (polyphenolic components, with a broad array of different structures (phenolic acids, flavonoids, and hydrolyzable tannins and a quick, high throughput, and accurate screening of its complete profile is still lacking. In the present work, a method for UHPLC separation and linear ion trap mass spectrometric (MSn characterization of pomegranate juice phenolic fraction was optimized by comparing several different analytical conditions. The best solutions for phenolic acids, anthocyanins, flavonoids, and ellagitannins have been delineated and more than 70 compounds have been identified and fully characterized in less than one hour total analysis time. Twenty-one compounds were tentatively detected for the first time in pomegranate juice. The proposed fingerprinting approach could be easily translated to other plant derived food extracts and beverages containing a wide array of phytochemical compounds.

  4. Two UGT84 Family Glycosyltransferases Catalyze a Critical Reaction of Hydrolyzable Tannin Biosynthesis in Pomegranate (Punica granatum)

    Ono, Nadia N.; Qin, Xiaoqiong; Wilson, Alexander E.; Li, Gang

    2016-01-01

    Hydrolyzable tannins (HTs) play important roles in plant herbivore deterrence and promotion of human health. A critical step in HT production is the formation of 1-O-galloyl-β-D-glucopyranoside (β-glucogallin, ester-linked gallic acid and glucose) by a UDP-glucosyltransferase (UGT) activity. We cloned and biochemically characterized four candidate UGTs from pomegranate (Punica granatum), of which only UGT84A23 and UGT84A24 exhibited β-glucogallin forming activities in enzyme assays. Although overexpression and single RNAi knockdown pomegranate hairy root lines of UGT84A23 or UGT84A24 did not lead to obvious alterations in punicalagin (the prevalent HT in pomegranate) accumulation, double knockdown lines of the two UGTs resulted in largely reduced levels of punicalagins and bis-hexahydroxydiphenyl glucose isomers. An unexpected accumulation of galloyl glucosides (ether-linked gallic acid and glucose) was also detected in the double knockdown lines, suggesting that gallic acid was utilized by an unidentified UGT activity for glucoside formation. Transient expression in Nicotiana benthamiana leaves and immunogold labeling in roots of pomegranate seedlings collectively indicated cytosolic localization of UGT84A23 and UGT84A24. Overall, functional characterization and localization of UGT84A23 and UGT84A24 open up opportunities for further understanding the regulatory control of HT metabolism in plants and its coordination with other biochemical pathways in the metabolic network. PMID:27227328

  5. Two UGT84 Family Glycosyltransferases Catalyze a Critical Reaction of Hydrolyzable Tannin Biosynthesis in Pomegranate (Punica granatum.

    Nadia N Ono

    Full Text Available Hydrolyzable tannins (HTs play important roles in plant herbivore deterrence and promotion of human health. A critical step in HT production is the formation of 1-O-galloyl-β-D-glucopyranoside (β-glucogallin, ester-linked gallic acid and glucose by a UDP-glucosyltransferase (UGT activity. We cloned and biochemically characterized four candidate UGTs from pomegranate (Punica granatum, of which only UGT84A23 and UGT84A24 exhibited β-glucogallin forming activities in enzyme assays. Although overexpression and single RNAi knockdown pomegranate hairy root lines of UGT84A23 or UGT84A24 did not lead to obvious alterations in punicalagin (the prevalent HT in pomegranate accumulation, double knockdown lines of the two UGTs resulted in largely reduced levels of punicalagins and bis-hexahydroxydiphenyl glucose isomers. An unexpected accumulation of galloyl glucosides (ether-linked gallic acid and glucose was also detected in the double knockdown lines, suggesting that gallic acid was utilized by an unidentified UGT activity for glucoside formation. Transient expression in Nicotiana benthamiana leaves and immunogold labeling in roots of pomegranate seedlings collectively indicated cytosolic localization of UGT84A23 and UGT84A24. Overall, functional characterization and localization of UGT84A23 and UGT84A24 open up opportunities for further understanding the regulatory control of HT metabolism in plants and its coordination with other biochemical pathways in the metabolic network.

  6. Prophylactic effects of pomegranate (Punica granatum) juice on sodium fluoride induced oxidative damage in liver and erythrocytes of rats.

    Bouasla, Asma; Bouasla, Ihcène; Boumendjel, Amel; Abdennour, Cherif; El Feki, Abdelfattah; Messarah, Mahfoud

    2016-07-01

    The objective of this study was to investigate the protective effects of pomegranate (Punica granatum) juice (PGJ) on oxidative damages in liver tissue and erythrocytes of rats intoxicated by sodium fluoride (NaF). Rats were randomly divided into two groups: group I received standard diet and group II received orally 1 mL of PGJ. After 5 weeks of pretreatment, each group was divided again into two subgroups and treated for another 3 weeks as follows: group I was subdivided into a control group and a group that was treated with 100 ppm of NaF (in drinking water); group II was subdivided into one group that was treated daily with both 100 ppm NaF and PGJ (1 mL orally) and one that received daily 1 mL of pomegranate juice. Exposure to NaF decreased hematological parameters, changed the total protein, albumin, bilirubin levels, and increased the activities of hepatic marker enzymes. We also noted an increase in lipid peroxidation contents, accompanied by a decrease of reduced glutathione levels. Antioxidant enzyme activities in both tissues were modified in the NaF group compared with the control group. However, the administration of PGJ juice caused an amelioration of the previous parameters. Our results indicated the potential effects of NaF to induce oxidative damage in tissues and the ability of PGJ to attenuate NaF-induced oxidative injury. PMID:27124270

  7. The Effectiveness of Cinnamomum zeylanicum, Punica granatum Flower and Capsicum annuum Extracts Against Parascaris equorum Infective Larvae

    Ehsan RAKHSHANDEHROO

    2016-07-01

    Full Text Available Recent investigations have shown that plants with medicinal peculiarities as good alternative to anthelmintics for livestock. In this study, the anthelmintic effects of three medicinal herbs (Cinnamomum zeylanicum, Punica granatum flower and Capsicum annuum were screened in vitro against the infective larvae of Parascaris equorum. The recovered larvae of the parasite were exposed to four concentrations (50, 75, 100 and 125 mg/mL of the extracts and then they examined for the viability at 0, 10, 20, 30 and 40 minutes after the challenge. The results revealed that all the concentrations of each plant extract had anthelmintic effects on P. equorum larvae. Also, the statistics indicated that there were significant interactions between the concentration of the extracts and time of exposure on the number of viable larvae. In addition, C. annuum extract seemed to be a strong potency to kill larvae at all concentrations from the beginning of the experiment. These results confirmed that those herbal extracts possess good antiparasitic effects against infective larvae of P. equorum and thus could be considered in anthelminth treatment strategies.

  8. Antidiabetic effect of Punica granatum flowers: effect on hyperlipidemia, pancreatic cells lipid peroxidation and antioxidant enzymes in experimental diabetes.

    Bagri, Priyanka; Ali, Mohd; Aeri, Vidhu; Bhowmik, Malay; Sultana, Shahnaz

    2009-01-01

    The present study investigated the effects of Punica granatum aqueous extract (PgAq) on streptozotocin (STZ) induced diabetic rats by measuring fasting blood glucose, lipid profiles (atherogenic index), lipid peroxidation (LPO) and activities of both non-enzymatic and enzymatic antioxidants. Diabetes was induced by single intraperitoneal injection of STZ (60 mg/kg) to albino Wistar rats. The increase in blood glucose level, total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), very low density lipoprotein (VLDL), LPO level with decrease in high density lipoprotein cholesterol (HDL-C), reduced glutathione (GSH) content and antioxidant enzymes namely, glutathione peroxidase (GPx), glutathione reductase (GR), glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) were the salient features observed in diabetic rats. On the other hand, oral administration of PgAq at doses of 250 mg/kg and 500 mg/kg for 21 days resulted in a significant reduction in fasting blood glucose, TC, TG, LDL-C, VLDL-C and tissue LPO levels coupled with elevation of HDL-C, GSH content and antioxidant enzymes in comparison with diabetic control group. The results suggest that PG could be used, as a dietary supplement, in the treatment of chronic diseases characterized by atherogenous lipoprotein profile, aggravated antioxidant status and impaired glucose metabolism and also in their prevention. PMID:18950673

  9. Punica granatum flower extract, a potent alpha-glucosidase inhibitor, improves postprandial hyperglycemia in Zucker diabetic fatty rats.

    Li, Yuhao; Wen, Suping; Kota, Bhavani Prasad; Peng, Gang; Li, George Qian; Yamahara, Johji; Roufogalis, Basil D

    2005-06-01

    Postprandial hyperglycemia plays an important role in the development of type 2 diabetes and has been proposed as an independent risk factor for cardiovascular diseases. The flowering part of Punica granatum Linn. (Punicaceae) (PGF) has been recommended in Unani literature as a remedy for diabetes. We investigated the effect and action mechanism of a methanolic extract from PGF on hyperglycemia in vivo and in vitro. Oral administration of PGF extract markedly lowered plasma glucose levels in non-fasted Zucker diabetic fatty rats (a genetic model of obesity and type 2 diabetes), whereas it had little effect in the fasted animals, suggesting it affected postprandial hyperglycemia in type 2 diabetes. In support of this conclusion the extract was found to markedly inhibit the increase of plasma glucose levels after sucrose loading, but not after glucose loading in mice, and it had no effect on glucose levels in normal mice. In vitro, PGF extract demonstrated a potent inhibitory effect on alpha-glucosidase activity (IC50: 1.8 microg/ml). The inhibition is dependent on the concentration of enzyme and substrate, as well as on the length of pretreatment with the enzyme. These findings strongly suggest that PGF extract improves postprandial hyperglycemia in type 2 diabetes and obesity, at least in part, by inhibiting intestinal alpha-glucosidase activity. PMID:15894133

  10. Amelioration of Diabetes and Painful Diabetic Neuropathy by Punica granatum L. Extract and Its Spray Dried Biopolymeric Dispersions

    K. Raafat

    2014-01-01

    Full Text Available Aims. To evaluate the effect of Punica granatum (Pg rind extract and its spray dried biopolymeric dispersions with casein (F1 or chitosan (F2 against Diabetes mellitus (DM and diabetic neuropathy (DN. Methods. We measured the acute (6 h and subacute (8 days effect of various doses of Pg, F1, and F2 and the active compounds on alloxan-induced DM mouse model. We evaluated DN utilizing latency tests for longer period of time (8 weeks. In addition, the in vivo antioxidant activity was assessed utilizing serum catalase level. Results. The results proved that the highest dose levels of Pg extract, F1, F2 exerted remarkable hypoglycemic activity with 48, 52, and 40% drop in the mice glucose levels after 6 hours, respectively. The tested compounds also improved peripheral nerve function as observed from the latency tests. Bioguided fractionation suggested that gallic acid (GA was Pg main active ingredient responsible for its actions. Conclusion. Pg extract, F1, F2, and GA could be considered as a new therapeutic potential for the amelioration of diabetic neuropathic pain and the observed in vivo antioxidant potential may be involved in its antinociceptive effect. It is highly significant to pay attention to Pg and GA for amelioration and control of DM and its complications.

  11. Two UGT84 Family Glycosyltransferases Catalyze a Critical Reaction of Hydrolyzable Tannin Biosynthesis in Pomegranate (Punica granatum).

    Ono, Nadia N; Qin, Xiaoqiong; Wilson, Alexander E; Li, Gang; Tian, Li

    2016-01-01

    Hydrolyzable tannins (HTs) play important roles in plant herbivore deterrence and promotion of human health. A critical step in HT production is the formation of 1-O-galloyl-β-D-glucopyranoside (β-glucogallin, ester-linked gallic acid and glucose) by a UDP-glucosyltransferase (UGT) activity. We cloned and biochemically characterized four candidate UGTs from pomegranate (Punica granatum), of which only UGT84A23 and UGT84A24 exhibited β-glucogallin forming activities in enzyme assays. Although overexpression and single RNAi knockdown pomegranate hairy root lines of UGT84A23 or UGT84A24 did not lead to obvious alterations in punicalagin (the prevalent HT in pomegranate) accumulation, double knockdown lines of the two UGTs resulted in largely reduced levels of punicalagins and bis-hexahydroxydiphenyl glucose isomers. An unexpected accumulation of galloyl glucosides (ether-linked gallic acid and glucose) was also detected in the double knockdown lines, suggesting that gallic acid was utilized by an unidentified UGT activity for glucoside formation. Transient expression in Nicotiana benthamiana leaves and immunogold labeling in roots of pomegranate seedlings collectively indicated cytosolic localization of UGT84A23 and UGT84A24. Overall, functional characterization and localization of UGT84A23 and UGT84A24 open up opportunities for further understanding the regulatory control of HT metabolism in plants and its coordination with other biochemical pathways in the metabolic network. PMID:27227328

  12. Purification and Antioxidant Activity of Polyphenols from Punica granatum L.%石榴叶多酚的纯化及抗氧化活性研究

    邢佳; 陆文娟; 赵云霞; 陶明煊

    2015-01-01

    采用大孔树脂对石榴叶粗多酚进行纯化,得到3种不同纯度的石榴叶多酚,对这3种多酚的抗氧化活性进行研究并与芦丁对比.结果显示,最佳纯化工艺条件为:上柱质量浓度2.5 mg/mL,pH=4,上柱速率2 mL/min,乙醇体积分数60%,洗脱量为1.7 BV~2.0 BV.经AB-8大孔树脂分离后,石榴叶多酚的纯度提高到62.44%,再经聚酰胺树脂分离后石榴叶多酚的纯度为92.83%.石榴叶多酚的抗氧化性研究结果表明,3种不同纯度的多酚均具有清除自由基的效果及还原能力,而且纯度越高清除自由基的效果越好,还原能力越强.与芦丁相比,纯化后的石榴叶多酚具有更好的还原力及清除亚硝酸盐的能力.%The macroporous resin was choosen for separating crude polyphenols from the leaves of Punica granatum L., three different purity of polyphenols from Punica granatum L. leaves were got,compared with Rutin,three different puri⁃ty of polyphenols from Punica granatum L. were evaluated with different antioxidant tests. The results showed that with sample solution concentration of 2.5 mg/mL,pH=4,feeding speed of 2 mL/min,ethanol concentration of 60%,elution volume of 1.7 BV~2.0 BV,the purity of CPPL was 62.44%,the purity of purified polyphenols of crude polyphenols from Punica granatum L. leaves was 92.83%which was further improved by PA. The results of oxidation stability analysis of polyphenols in Punica granatum L. leaves were showed that it had the better effect in scavenging free radical and reduc⁃ing power with the higher purity,compared with rutin,reducing power and nitrite scavenging activity of purified polyphe⁃nols from Punica granatum L. leaves was stronger.

  13. Pharmacological investigations of Punica granatum in glycerol-induced acute renal failure in rats

    Amrit Pal Singh; Amteshwar Jaggi Singh; Nirmal Singh

    2011-01-01

    Objective : The present study was designed to investigate the ameliorative potential and possible mechanism of hydroalcoholic extract of flowers of P. granatum in glycerol-induced acute renal failure (ARF) in rats. Materials and Methods : The rats were subjected to rhabdomyolytic ARF by single intramuscular injection of hypertonic glycerol (50% v/v; 8 ml/kg) and the animals were sacrificed after 24 hours of glycerol injection. The plasma creatinine, blood urea nitrogen, creatinine clearance, ...

  14. Ellagitannins of the fruit rind of pomegranate (Punica granatum antagonize in vitro the host inflammatory response mechanisms involved in the onset of malaria

    Bhattacharya Deepak

    2010-07-01

    Full Text Available Abstract Background The sun-dried rind of the immature fruit of pomegranate (Punica granatum is presently used as a herbal formulation (OMARIA, Orissa Malaria Research Indigenous Attempt in Orissa, India, for the therapy and prophylaxis of malaria. The pathogenesis of cerebral malaria, a complication of the infection by Plasmodium falciparum, is an inflammatory cytokine-driven disease associated to an up-regulation and activity of metalloproteinase-9 and to the increase of TNF production. The in vitro anti-plasmodial activity of Punica granatum (Pg was recently described. The aim of the present study was to explore whether the anti-malarial effect of OMARIA could also be sustained via other mechanisms among those associated to the host immune response. Methods From the methanolic extract of the fruit rind, a fraction enriched in tannins (Pg-FET was prepared. MMP-9 secretion and expression were evaluated in THP-1 cells stimulated with haemozoin or TNF. The assays were conducted in the presence of the Pg-FET and its chemical constituents ellagic acid and punicalagin. The effect of urolithins, the ellagitannin metabolites formed by human intestinal microflora, was also investigated. Results Pg-FET and its constituents inhibited the secretion of MMP-9 induced by haemozoin or TNF. The effect occurred at transcriptional level since MMP-9 mRNA levels were lower in the presence of the tested compounds. Urolithins as well inhibited MMP-9 secretion and expression. Pg-FET and pure compounds also inhibited MMP-9 promoter activity and NF-kB-driven transcription. Conclusions The beneficial effect of the fruit rind of Punica granatum for the treatment of malarial disease may be attributed to the anti-parasitic activity and the inhibition of the pro-inflammatory mechanisms involved in the onset of cerebral malaria.

  15. Comparing the Extraction Methods of Punica granatum Seed Oil%石榴种籽油提取方法的比较

    杨万政; 路静; 杜晓鸣; 汪丽萍

    2011-01-01

    Objective To optimize the extracting technology of seed oil from Punica Granatum. Methods 4 methods: Supercritical CO2, ultrasonic wave, microwave and solvent reflux were used to extract Punica granatum seed oil. Extraction results were compared to determine the optimal method. Results Supercritical CO2 proved to be the best method. The optimal condition was as follows: extraction press, 30 Mpa; separation press, 10 Mpa; extraction temperature, 50℃; separation temperature, 40 ℃. And extraction time, 2 h. The extraction rate was above 17%. Conclusion Analysis of oil content and fat acids constitution in the seeds shows the fat acid in Punica granatum seeds consist mainly of C18 punicic acid.%目的 研究提取石榴籽油的最佳工艺.方法 采用超临界CO2流体萃取、超声波萃取、微波萃取、溶剂回流萃取四种方法提取石榴种籽中的油脂.结果 确定最佳条件为:超临界CO2流体萃取,当萃取压力30 Mpa,分离压力:10Mpa,萃取温度:50℃,分离温度:40℃,提取2h,提取率可达17%以上.结论 通过对种籽中油脂含量及脂肪酸组成的分析,石榴的脂肪酸主要由C18的石榴酸组成.

  16. Study of nutrient composition in pollen of Punica granatum L.%安石榴花粉产量及营养成分

    张秀春

    2001-01-01

    The authors report the yield and nutrient composition of pollen which were yielded by Punica granatum L. The results show that not only the plant's is rich, but also its nutrient composition is rich Its value of explotation and utitilization is very well.%对安石榴的产量及蛋白质、氨基酸等营养成分进行了分析测定;结果表明安石榴花粉资源丰富,营养成分齐全,具有很好的开发利用价值。

  17. The Investigation for Antifungal Effect of Medicinal Plants, Combination Infusion of Piper Bettle Leaves, Punica Granatum Fructus Cortec, Curcuma Domestica Rhizome

    Berna Elya; Atiek Soemiati

    2002-01-01

    The tested demartophyta used Candida albicans. This research using dilution method to determine of minimum inhibitory concentration (MIC) and difusion method to determine zone inhibition around of disc. The results of determination of MIC showed that combination infusion Piper bettle leaves with Punica granatum Fructus Cortex against C. albicans respectively, 31,2 mg/ml : 7,8 mg/ml; 15,6 mg/ml : 15,6 mg/ml and 7,8 mg/ml : 31,2 mg/ml. The determination for zone inhibition from 3 concentration ...

  18. Potential Anti-Inflammatory Effects of the Hydrophilic Fraction of Pomegranate (Punica granatum L.) Seed Oil on Breast Cancer Cell Lines

    Susan Costantini; Fabiola Rusolo; Valentina De Vito; Stefania Moccia; Gianluca Picariello; Francesca Capone; Eliana Guerriero; Giuseppe Castello; Maria Grazia Volpe

    2014-01-01

    In this work, we characterized conjugated linolenic acids (e.g., punicic acid) as the major components of the hydrophilic fraction (80% aqueous methanol extract) from pomegranate (Punica granatum L.) seed oil (PSO) and evaluated their anti-inflammatory potential on some human colon (HT29 and HCT116), liver (HepG2 and Huh7), breast (MCF-7 and MDA-MB-231) and prostate (DU145) cancer lines. Our results demonstrated that punicic acid and its congeners induce a significant decrease of cell viabili...

  19. Germinação de sementes de romäzeiras (Punica granatum L.) de acordo com a concentração de giberelina

    William Takata; Ewerton Gasparetto da Silva; Jaqueline Malagutti Corsato; Gisela Ferreira

    2014-01-01

    A romäzeira (Punica granatum L.) é fruteira de porte arbustivo, utilizada geralmente como planta ornamental e explorada quanto a suas características medicinais. Seus frutos apresentam cavidades internas onde estäo alojadas numerosas sementes e sem a presença de endosperma. Estas sementes apresentam certa dificuldade para germinar, sendo que se conseguiu atingir porcentagens satisfatórias de germinação com a utilização de estratificação, que é processo necessário para a quebra da dormência fi...

  20. Caracteres morfoanatómicos de diferentes cortezas de la medicina folklórica argentina : II. Punica granatum L. (Punicaceae) y su adulterante Buxus sempervirens L. (Buxaceae)

    Di Sapio Osvaldo Antonio; Gattuso, Martha

    1994-01-01

    Se presenta un estudio comparativo, morfoanatómico e histoquímico, de la corteza de raíz y tailo de Punica granatum L. (Punicaceae), "corteza de granado" -utilizada en medicina popular por sus propiedades tenífugas y antiheimínticas- y de su adulterante, Buxus sempervirens L. (Buxaceae), "boj". El análisis efectuado posibilita la confección de un cuadro de caracteres con valor diagnóstico. Estos permiten la identificación de las citadas especies al estado de droga entera o fragmentada.

  1. High-yielding Horticultural Technique for Punica granatum%蒙自石榴丰产栽培技术研究

    李品荣; 陈强; 常恩福; 周洪昌; 王锡全; 李红兵; 李玉文; 刘永国; 徐珑峰

    2001-01-01

    @@ 蒙自石榴(Punica granatum)早实丰产,是山区群众脱贫致富的最佳经济林树种之一.本研究通过对蒙自石榴的丰产栽培技术措施进行田间试验,寻找较佳的技术组合,为滇东南岩溶山区的经济发展提供技术支撑. 1试验地概况及方法 1.1试验地概况

  2. 濒危品种软籽石榴的组织培养和快速繁殖%Tissue Culture and Rapid Propagation of Punica granatum

    闫志佩

    2004-01-01

    1植物名称 石榴(Punica granatum)。2材料类别 新梢茎尖。3培养条件 (1)芽诱导培养基:MS+6.BA1.5mg·L-1(单位下同)+IBA0.2+NAA0.1;(2)芽增殖培养基:MS+6-BA2.0+IBA0.5;(3)生根培养基:

  3. Mikrobna proizvodnja antioksidativnih dodataka hrani na čvrstoj podlozi od kore šipka (Punica granatum) i lišća grma Larrea tridentata

    Aguilar, Cristóbal Noé; Aguilera-Carbo, Antonio; Robledo, Armando; Ventura, Janeth; Belmares, Ruth; Martinez, Diego; Rodríguez-Herrera, Raul; Contreras, Juan

    2008-01-01

    U radu je istražena mogućnost primjene lišća grma Larrea tridentata i kore šipka (Punica granatum) kao sirovina za dodatak čvrstim podlogama te proizvodnju antioksidanasa. U središtu je istraživanja kinetička procjena fizikalno-kemijskih promjena tih taninima bogatih sirovina, ističući promjenu udjela polifenolnih spojeva tijekom procesa. Za proces je upotrijebljen soj plijesni Aspergillus niger GH1, koji je praćen tijekom 96 sati. Određen je udio proteina, sirovih vlakana, lipida, te ukupnih...

  4. Research progress on pharmacological activities of flowers ofPunica granatum%石榴花的药理作用研究进展

    赵永昕; 热娜·卡斯木

    2015-01-01

    Punica granatum L. has a long medicinal usage history, and the different parts ofP. granatum are very popular in traditional Chinese medicine and other ethnic medicine. In recent years, the pharmacological research of flowers ofP. granatum on antioxidation, hypoglycemic action, and hypolipidemic effect have proceeded rapidly. The scientific collection and analysis on effects and new pharmacological research results of flowers ofP. granatum are reviewed, in order to provide theoretical foundation for the comprehensive development and utilization of resource.%石榴药用历史悠久,不同部位在中医药与民族医药中应用广泛。近年来,有关石榴花的抗氧化、降血糖及调血脂等药理研究进展迅速,通过整理归纳并科学的分析石榴花的功效及其新的药理学研究成果,以期为石榴花的进一步研究和资源的合理利用提供理论依据。

  5. Avaliação da atividade antioxidante em diferentes extratos da polpa e sementes da romã (Punica granatum, L. Antioxidant activity evaluation of different polarities extracts by pulp and seeds of pomegranate (Punica granatum, L.

    Fernanda Archilla Jardini

    2007-03-01

    Full Text Available Avaliou-se a atividade antioxidante dos extratos etéreo, alcoólico e aquoso obtidos da polpa e das sementes da romã (Punica granatum, L., utilizando-se dois sistemas: 1 co-oxidação do beta-caroteno/ácido linoléico e 2 Rancimat®. Inicialmente foram determinados os compostos redutores presentes na polpa (1.214 mg/100 g e nas sementes (1.732 mg/100 g e, em seqüência, avaliou-se nos extratos, através de cromatografia em camada delgada (CCD, a presença de compostos fenólicos com atividade antioxidante. Os extratos aquosos tanto da polpa quanto das sementes apresentaram as maiores porcentagens de inibição da oxidação: 87,31% e 93,08%, respectivamente. Através da avaliação cinética pelo teste de co-oxidação com beta-caroteno e ácido linoléico, pôde-se verificar que a alta porcentagem da atividade antioxidante dos extratos aquosos é devida à capacidade de inibir a oxidação nas fases inicial e mais avançada do processo oxidativo, agindo por mecanismos primário e secundário. Todos os extratos (etéreo, alcoólico e aquoso apresentaram elevada capacidade em prolongar o período de indução da oxidação, medida pelo aparelho Rancimat®, sendo, ainda, significativamente (pIn etheric, alcoholic and aqueous extracts obtained from pomegranate (Punica granatum, L. pulp and seeds their antioxidant activity was evaluated by using two systems: co-oxidation of beta-carotene/linoleic acid and 2 Rancimat®. First of all, the presence of reducing compounds was detected in the pulp (1,214 mg/100 g and in the seeds (1,732 mg/100 g. The phenolic compounds with antioxidant activity were then evaluated in the extracts using thin-layer chromatography (TLC. The aqueous extracts from the pulp and the seeds showed the highest antioxidant activity of 87.31% and of 93.08%, respectively. The kinetic curves obtained by cooxidation of beta-carotene and linoleic acid substrates also showed the highest values in the aqueous. These results were

  6. Anti-diabetic action of Punica granatum flower extract: Activation of PPAR-γ and identification of an active component

    Peroxisome proliferator-activated receptor (PPAR)-γ activators are widely used in the treatment of type 2 diabetes because they improve the sensitivity of insulin receptors. Punica granatum flower (PGF) has been used as an anti-diabetic medicine in Unani medicinal literature. The mechanism of actions is, however, unknown. In the current study, we demonstrated that 6-week oral administration of methanol extract from PGF (500 mg/kg, daily) inhibited glucose loading-induced increase of plasma glucose levels in Zucker diabetic fatty rats (ZDF), a genetic animal model for type 2 diabetes, whereas it did not inhibit the increase in Zucker lean rats (ZL). The treatment did not lower the plasma glucose levels in fasted ZDF and ZL rats. Furthermore, RT-PCR results demonstrated that the PGF extract treatment in ZDF rats enhanced cardiac PPAR-γ mRNA expression and restored the down-regulated cardiac glucose transporter (GLUT)-4 (the insulin-dependent isoform of GLUTs) mRNA. These results suggest that the anti-diabetic activity of PGF extract may result from improved sensitivity of the insulin receptor. From the in vitro studies, we demonstrated that the PGF extract enhanced PPAR-γ mRNA and protein expression and increased PPAR-γ-dependent mRNA expression and activity of lipoprotein lipase in human THP-1-differentiated macrophage cells. Phytochemical investigation demonstrated that gallic acid in PGF extract is mostly responsible for this activity. Thus, our findings indicate that PPAR-γ is a molecular target for PGF extract and its prominent component gallic acid, and provide a better understanding of the potential mechanism of the anti-diabetic action of PGF

  7. Protective effects of Punica Granatum (L) and synthetic ellagic acid on radiation induced biochemical alterations in Swiss albino mice

    Ionizing radiations produce deleterious effects in the living organisms and the rapid technological advancement has increased human exposure to ionizing radiations enormously. Radiotherapy, which is a chief modality to treat cancer, faces a major drawback because it produces severe side effects developed due to damage to normal tissue by reactive oxygen species (ROS). Recent studies have indicated that some commonly used medicinal plants may be good sources of potent but non-toxic radioprotectors. The pomegranate, Punica granatum L., an ancient, mystical, and highly distinctive fruit, is the predominant member of the Punicaceae family. It is used in several systems of medicine for a variety of ailments. The objective of the present study was to investigate the protective effects of ethanolic extracts of pomegranate whole fruit (EPWF) and seeds (EPS) and Synthetic Ellagic acid (EA) against Electron beam radiation(EBR) induced biochemical alterations in Swiss albino mice. The extracts and synthetic compound were assessed for its radical scavenging property by DPPH radical scavenging and Ferric Reducing Antioxidant Power assays. The animals were exposed to sub-lethal dose (6 Gy) of Electron Beam Radiation and then treated with 200 mg/kg body wt. of pomegranate extracts and synthetic ellagic acid for 15 consecutive days. The biochemical estimations were carried out in the liver homogenate of the sacrificed animals. Radiation induced depletion in the level of reduced glutathione and total antioxidant capacity were prevented significantly by EPWF, EPS and EA administration. Also there was significant reduction in the levels of membrane lipid peroxidation in the treated groups compared to irradiated control. The findings of our study indicate the protective efficacy of pomegranate extracts and synthetic ellagic acid on radiation induced biochemical changes in mice may be due to its free radical scavenging and increased antioxidant levels. (author)

  8. Anti-diabetic action of Punica granatum flower extract: activation of PPAR-gamma and identification of an active component.

    Huang, Tom H W; Peng, Gang; Kota, Bhavani P; Li, George Q; Yamahara, Johji; Roufogalis, Basil D; Li, Yuhao

    2005-09-01

    Peroxisome proliferator-activated receptor (PPAR)-gamma activators are widely used in the treatment of type 2 diabetes because they improve the sensitivity of insulin receptors. Punica granatum flower (PGF) has been used as an anti-diabetic medicine in Unani medicinal literature. The mechanism of actions is, however, unknown. In the current study, we demonstrated that 6-week oral administration of methanol extract from PGF (500 mg/kg, daily) inhibited glucose loading-induced increase of plasma glucose levels in Zucker diabetic fatty rats (ZDF), a genetic animal model for type 2 diabetes, whereas it did not inhibit the increase in Zucker lean rats (ZL). The treatment did not lower the plasma glucose levels in fasted ZDF and ZL rats. Furthermore, RT-PCR results demonstrated that the PGF extract treatment in ZDF rats enhanced cardiac PPAR-gamma mRNA expression and restored the down-regulated cardiac glucose transporter (GLUT)-4 (the insulin-dependent isoform of GLUTs) mRNA. These results suggest that the anti-diabetic activity of PGF extract may result from improved sensitivity of the insulin receptor. From the in vitro studies, we demonstrated that the PGF extract enhanced PPAR-gamma mRNA and protein expression and increased PPAR-gamma-dependent mRNA expression and activity of lipoprotein lipase in human THP-1-differentiated macrophage cells. Phytochemical investigation demonstrated that gallic acid in PGF extract is mostly responsible for this activity. Thus, our findings indicate that PPAR-gamma is a molecular target for PGF extract and its prominent component gallic acid, and provide a better understanding of the potential mechanism of the anti-diabetic action of PGF. PMID:16102567

  9. Un extracto de Punica granatum L. (BLBu sobre la apoptosis inducida por virus influenza A y B

    Isis Casadelvalle Pérez

    2006-01-01

    Full Text Available La apoptosis ha sido ampliamente reconocida como un mecanismo intrínseco de autodestrucción en las células animales. Este fenómeno puede ser inducido por múltiples factores, entre los que se pueden citar algunos virus, que lo utilizan como vía de diseminación en el hospedero. Para contrarrestar este mecanismo se han usado extractos obtenidos de plantas con propiedades antivirales, los cuales, intuitivamente, pudieran ejercer su efecto sobre la apoptosis inducida por los virus. En el presente trabajo se estudió el efecto de un extracto natural de Punica granatum L. sobre la apoptosis inducida por dos virus: influenza A e influenza B en dos líneas celulares: MDCK y células Vero. Se determinó la citotoxicidad del extracto y se estimó que el valor de CC50 para la línea celular MDCK resulta de (400 ± 59 mg/mL y en las células Vero de (137,3 ± 23 mg/mL. A concentraciones no citotóxicas el extracto no indujo la fragmentación del ADN. El efecto del extracto sobre las células infectadas con los virus influenza A/ Japan/10/99(H3N2, influenza B/Beijing/88 fue evaluado mediante la técnica del MTT y el análisis de la fragmentación del ADN, técnicas usadas para identificar el proceso de muerte apoptótica. El proceso de apoptosis fue inhibido por el extracto a todas las concentraciones y tiempos ensayados. A partir de los resultados, se infiere la existencia de mecanismos comunes de inducción de la apoptosis por estos virus que son interferidos por el extracto natural ensayado.

  10. Biogenic robust synthesis of silver nanoparticles using Punica granatum peel and its application as a green catalyst for the reduction of an anthropogenic pollutant 4-nitrophenol

    Edison, T. Jebakumar Immanuel; Sethuraman, M. G.

    2013-03-01

    A robust synthesis of silver nanoparticles (AgNPs) using the peel extract of Punica granatum is reported in this article. The formation of AgNPs was confirmed by the appearance of brownish yellow color and the Surface Plasmon Resonance (SPR) peak at 432 nm. The biogenic AgNPs were found to have the size approximately 30 nm with distorted spherical shape. The high negative zeta potential values of AgNPs revealed their high stability which could be attributed to the capping of AgNPs by the phytoconstituents of the Punica granatum peel. The biogenic AgNPs were also found to function as an effective green catalyst in the reduction of anthropogenic pollutant viz., 4-nitrophenol (4-NP) by solid sodium borohydride, which was evident from the instantaneous color change of bright yellow (400 nm) to colorless (294 nm) solution, after the addition of AgNPs. The catalytic action of biogenic AgNPs in the reduction of 4-NP could be explained on the basis of Langmuir-Hinshelwood model.

  11. The High-yield Cultivation Techniques for Soft-seed Punica granatum from Tunisia%突尼斯软子石榴丰产栽培技术

    吕中伟; 王鹏; 王东升; 刘许成; 许领军

    2009-01-01

    Through six-year examination in cultivation, the main variety soft-seed Punica granatum from Tunisia should be matched with pollinizer as the proportion of 6:1. The cultivation techniques were severely thinning blossom and fruit, pruning according to branch trait, adopting frost protection measures in the winter and so on. The soft-seed Punica granatum from Tunisia was of favorable varietal characteristics, fruit properties and economic benefits compared with other varieties. It had better market prospect and broader space for further development.%经过6年栽培试验,突尼斯软子石榴栽培上需要配置授粉树(6:1),严格疏花疏果,根据枝条特性整形修剪,冬季要采取防冻措施等.表现出良好的品种特性,果实性状、经济效益远高于其他品种,具有良好的市场前景,有很大的发展空间.

  12. Estudos com o extrato da Punica granatum Linn. (romã): efeito antimicrobiano in vitro e avaliação clínica de um dentifrício sobre microrganismos do biofilme dental = Studies with the extract of the punica granatum linn. (pomegranate): effect antimicrobial “in vitro” and trial avaliation of a toothpaste upon microrganisms of the oral biofilm

    Pereira, Jozinete Vieira

    2005-01-01

    A atividade antimicrobiana in vitro, CIM (Concentração Inibitória Mínima), CIMA (Concentração Inibitória Mínima de Aderência) e a CBM (Concentração Mínima Bactericida) do extrato da romã (Punica granatum Linn. ) foi avaliada em cinco linhagens bacterianas do biofilme dental: Streptococcus mutans, Streptococcus mitis, Streptococcus sanguis, Streptococcus sobrinus e Lactobacillus casei. Em estudo comparativo com o gluconato de clorexidina, os resultados demonstraram efetiva ação inibitória de a...

  13. Punica granatum (pomegranate) flower extract possesses potent antioxidant activity and abrogates Fe-NTA induced hepatotoxicity in mice.

    Kaur, Gurpreet; Jabbar, Zoobi; Athar, Mohammad; Alam, M Sarwar

    2006-07-01

    Most pomegranate (Punica granatum Linn., Punicaceae) fruit parts are known to possess enormous antioxidant activity. The present study evaluated antioxidant and hepatoprotective activity of pomegranate flowers. Alcoholic (ethanolic) extract of flowers was prepared and used in the present study. The extract was found to contain a large amount of polyphenols and exhibit enormous reducing ability, both indicative of potent antioxidant ability. The extract showed 81.6% antioxidant activity in DPPH model system. The ability of extract to scavenge reactive oxygen species (ROS) and reactive nitrogen species (RNS) was tested and it was found to significantly scavenge superoxide (O(2)(.-)) (by up to 53.3%), hydrogen peroxide (H(2)O(2)) (by up to 30%), hydroxyl radicals (()OH) (by up to 37%) and nitric oxide (NO) (by up to 74.5%). The extract also inhibited (.)OH induced oxidation of lipids and proteins in vitro. These results indicated pomegranate flower extract to exert a significant antioxidant activity in vitro. The efficacy of extract was tested in vivo and it was found to exhibit a potent protective activity in acute oxidative tissue injury animal model: ferric nitrilotriacetate (Fe-NTA) induced hepatotoxicity in mice. Intraperitoneal administration of 9 mg/kg body wt. Fe-NTA to mice induced oxidative stress and liver injury. Pretreatment with pomegranate flower extract at a dose regimen of 50-150 mg/kg body wt. for a week significantly and dose dependently protected against Fe-NTA induced oxidative stress as well as hepatic injury. The extract afforded up to 60% protection against hepatic lipid peroxidation and preserved glutathione (GSH) levels and activities of antioxidant enzymes viz., catalase (CAT), glutathione peroxidase (GPX) glutathione reductase (GR) and glutathione-S-transferase (GST) by up to 36%, 28.5%, 28.7%, 40.2% and 42.5% respectively. A protection against Fe-NTA induced liver injury was apparent as inhibition in the modulation of liver markers viz

  14. Medicinal flowers. XXIII. New taraxastane-type triterpene, punicanolic acid, with tumor necrosis factor-alpha inhibitory activity from the flowers of Punica granatum.

    Xie, Yuanyuan; Morikawa, Toshio; Ninomiya, Kiyofumi; Imura, Katsuya; Muraoka, Osamu; Yuan, Dan; Yoshikawa, Masayuki

    2008-11-01

    The methanolic extract from the flowers of Punica granatum L. (Punicaceae) was found to show inhibitory effect on tumor necrosis factor-alpha (TNF-alpha, 1 ng/ml)-induced cytotoxicity in L929 cells. By bioassay-guided separation, a new taraxastane-type triterpene, punicanolic acid (1), was isolated from the active fraction (ethyl acetate-soluble fraction) together with four triterpenes (2--5), two galloyl glucoses (6, 7), two flavones (8, 9), and beta-sitosterol. Among the constituents, 1, oleanolic acid (2), maslinic acid (4), 1,2,6-tri-O-galloyl beta-D-glucopyranoside (6), 1,2-di-O-galloyl-4,6-O-(S)-hexahydroxydiphenoyl beta-D-glucopyranoside (7), and luteolin (8) significantly inhibited TNF-alpha-induced cytotoxicity in L929 cells at 30 microM. PMID:18981621

  15. CONTRIBUTION A LA VALORISATION ALIMENTAIRE DE LA GRENADE MAROCAINE (Punica Granatum L.) : CARACTERISATION PHYSICOCHIMIQUE, BIOCHIMIQUE ET STABILITE DE LEUR JUS FRAIS

    Hmid, Ilham

    2013-01-01

    Le grenadier (Punica granatum) est adapté aux climats arides et semi-arides d'où l'intérêt d'étudier et valoriser ses fruits à cause de l'accentuation des problèmes de sécheresse qui touchent le Maroc ces dernières années. D'un autre côté, la grenade possède de fortes capacités antioxydantes et anti-inflammatoires liées à la présence d'anthocyanes, de tanins et d'acide ellagique qui est utilisé en thérapeutique pour sa capacité à inhiber la prolifération des cellules cancéreuses. Toutefois, l...

  16. Green approach to corrosion inhibition of mild steel in two acidic solutions by the extract of Punica granatum peel and main constituents

    Behpour, M., E-mail: m.behpour@kashanu.ac.ir [Department of Analytical Chemistry, Faculty of Chemistry, University of Kashan, Kashan, I.R. 87317-51167 (Iran, Islamic Republic of); Ghoreishi, S.M.; Khayatkashani, M. [Department of Analytical Chemistry, Faculty of Chemistry, University of Kashan, Kashan, I.R. 87317-51167 (Iran, Islamic Republic of); Soltani, N. [Department of Chemistry, Payame Noor University (PNU), 19395-4697 Tehran (Iran, Islamic Republic of)

    2012-01-05

    Graphical abstract: Ellagic acid (EA) and tannic acid (TA) were studied as corrosion inhibitors. The electron density HOMO and LUMO of EA and TA were used to explain difference in behavior of them. Highlights: Black-Right-Pointing-Pointer The extract of Punica granatum (PG) and their main constituent (ellagic acid (EA)) are found to be good inhibitors for the corrosion of mild steel in 1 M H{sub 2}SO{sub 4} and 2 M HCl. Black-Right-Pointing-Pointer The electrochemical inhibitive mechanism is explained by potentiodynamic polarization curves and electrochemical impedance spectroscopy (EIS) results. Black-Right-Pointing-Pointer The adsorption of ALLOX on mild steel surface was found to accord with the Temkin adsorption isotherm. Black-Right-Pointing-Pointer The effect of temperature on the corrosion behavior of mild steel in 2 M HCl and 1 M H{sub 2}SO{sub 4} without and with the PG extract was studied. - Abstract: The effect of the extract of Punica granatum (PG) and their main constituents involve ellagic acid (EA) and tannic acid (TA), as mild steel corrosion inhibitor in 2 M HCl and 1 M H{sub 2}SO{sub 4} solutions was investigated by weight loss measurements. The results obtained from the weight loss measurements show that the inhibition efficiency of TA even in high concentration is very low. Thus, potentiodynamic polarization and electrochemical impedance spectroscopy (EIS) investigations were used for different concentrations of PG and EA and best concentration of TA. Potentiodynamic polarization curves indicated that PG and EA behave as mixed-type inhibitors. EIS measurements show an increase of the transfer resistance with increasing inhibitor concentration. The temperature effect on the corrosion behavior of steel without and with the PG extract was studied. The inhibition action of the extract was discussed in view of Langmuir adsorption isotherm.

  17. Green approach to corrosion inhibition of mild steel in two acidic solutions by the extract of Punica granatum peel and main constituents

    Graphical abstract: Ellagic acid (EA) and tannic acid (TA) were studied as corrosion inhibitors. The electron density HOMO and LUMO of EA and TA were used to explain difference in behavior of them. Highlights: ► The extract of Punica granatum (PG) and their main constituent (ellagic acid (EA)) are found to be good inhibitors for the corrosion of mild steel in 1 M H2SO4 and 2 M HCl. ► The electrochemical inhibitive mechanism is explained by potentiodynamic polarization curves and electrochemical impedance spectroscopy (EIS) results. ► The adsorption of ALLOX on mild steel surface was found to accord with the Temkin adsorption isotherm. ► The effect of temperature on the corrosion behavior of mild steel in 2 M HCl and 1 M H2SO4 without and with the PG extract was studied. - Abstract: The effect of the extract of Punica granatum (PG) and their main constituents involve ellagic acid (EA) and tannic acid (TA), as mild steel corrosion inhibitor in 2 M HCl and 1 M H2SO4 solutions was investigated by weight loss measurements. The results obtained from the weight loss measurements show that the inhibition efficiency of TA even in high concentration is very low. Thus, potentiodynamic polarization and electrochemical impedance spectroscopy (EIS) investigations were used for different concentrations of PG and EA and best concentration of TA. Potentiodynamic polarization curves indicated that PG and EA behave as mixed-type inhibitors. EIS measurements show an increase of the transfer resistance with increasing inhibitor concentration. The temperature effect on the corrosion behavior of steel without and with the PG extract was studied. The inhibition action of the extract was discussed in view of Langmuir adsorption isotherm.

  18. Estudos com o extrato da Punica granatum Linn. (romã: efeito antimicrobiano in vitro e avaliação clínica de um dentifrício sobre microrganismos do biofilme dental = Studies with the extract of the punica granatum linn. (pomegranate: effect antimicrobial “in vitro” and trial avaliation of a toothpaste upon microrganisms of the oral biofilm

    Pereira, Jozinete Vieira

    2005-01-01

    Full Text Available A atividade antimicrobiana in vitro, CIM (Concentração Inibitória Mínima, CIMA (Concentração Inibitória Mínima de Aderência e a CBM (Concentração Mínima Bactericida do extrato da romã (Punica granatum Linn. foi avaliada em cinco linhagens bacterianas do biofilme dental: Streptococcus mutans, Streptococcus mitis, Streptococcus sanguis, Streptococcus sobrinus e Lactobacillus casei. Em estudo comparativo com o gluconato de clorexidina, os resultados demonstraram efetiva ação inibitória de ambas as substâncias. O extrato da romã foi efetivo na CIMA das cinco linhagens ensaiadas, representada pela ausência de aderência ao vidro na presença de sacarose. A atividade antimicrobiana in vivo do extrato da romã foi testada em 13 pacientes, através do uso de um dentrifício a base desse extrato e foi observadas redução do número de Streptococcus mutans (53,84% e Índice de Sangramento Gengival. Conclui-se, portanto, que o extrato da Punica granatum Linn. , apresentou potente atividade antimicrobiana in vivo e in vitro sobre as linhagens do biofilme dental. Os resultados sugerem a utilização do extrato da romã em indicações terapêuticas na prática odontológica

  19. 石榴皮鞣质的急性毒性研究%Study of Acute Toxicity of Punica granatum Tannin

    邱红梅; 赖舒; 尚京川; 周岐新

    2011-01-01

    OBJECTIVE: To study the acute toxicity of Punica granatum tannins. METHODS: Mice were randomly divided into 7 groups and given P. granatum tannins 15 963.00 mg·kg-1, 12 978.00 mg·kg-1, 10 551.12 mg·kg-1,8 578.25 mg·kg-1,6 974.19 mg·kg-1, 5 670.07 mg·kg-1 and 4 609.81 mg·kg-1 via intragastric administration. Death cases were observed and recorded twice a day (8 am and 2 pm) for 7d. Pathological changes of liver tissue were observed. RESULTS: The LD50 and 95% confidence limit of P. granatum tannins were 8 469.28 mg· kg-1 and 7 252.70~9 685.83 mg·kg-1, respectively. Injury and necrosis of liver cells were observed in dose-dependent manner. CONCLUTION: High-dose of P. granatum tannins could cause liver damage and death in mice.%目的:研究石榴皮鞣质的急性毒性反应.方法:小鼠随机分为7个组,分别灌胃给予15 963.00、12 978.00、10 551.12、8 578.25、6 974.19、5 670.07、4 609.81 mg·kg-1石榴皮鞣质,于给药后每天8时、14时观察并记录其死亡情况,连续观察7 d;同时观察肝脏病理学改变.结果:石榴皮鞣质的半数致死量(LD50)为8 469.28 mg·kg-1,其95%可信限范围为7 252.70~9 685.83 mg·kg~;病理学改变显示,随剂量增高出现剂量依赖性肝细胞损伤、坏死.结论:石榴皮鞣质高剂量应用可造成小鼠肝脏损伤并导致死亡.

  20. Authentication of Punica granatum L.: Development of SCAR markers for the detection of 10 fruits potentially used in economically motivated adulteration.

    Marieschi, Matteo; Torelli, Anna; Beghé, Deborah; Bruni, Renato

    2016-07-01

    The large commercial success of pomegranate increase the likelihood of economically motivated adulteration (EMA), which has been gradually spotted with the undeclared addition of anthocyanin-rich plants or cheaper fruit juices used as bulking and diluting agents. A method based on Sequence-Characterized Amplified Regions (SCARs) was developed to detect the presence of Aristotelia chilensis, Aronia melanocarpa, Dioscorea alata, Euterpe oleracea, Malus×domestica, Morus nigra, Sambucus nigra, Vaccinium macrocarpon, Vaccinium myrtillus, Vitis vinifera as bulking agents in Punica granatum. The method enabled the unequivocal detection of up to 1% of each adulterant, allowing the preemptive rejection of suspect samples. The recourse to such method may reduce the number of samples to be subjected to further phytochemical analyses when multiple batches have to be evaluated in a short time. Vice versa, it allows the cross-check of suspect batches previously tested only for their anthocyanin profile. The dimension of the amplicons is suitable for the analysis of degraded DNA obtained from stored and processed commercial material. Proper SCAR markers may represent a fast, sensitive, reliable and low-cost screening method for the authentication of processed commercial pomegranate material. PMID:26920316

  1. Potential Anti-Inflammatory Effects of the Hydrophilic Fraction of Pomegranate (Punica granatum L. Seed Oil on Breast Cancer Cell Lines

    Susan Costantini

    2014-06-01

    Full Text Available In this work, we characterized conjugated linolenic acids (e.g., punicic acid as the major components of the hydrophilic fraction (80% aqueous methanol extract from pomegranate (Punica granatum L. seed oil (PSO and evaluated their anti-inflammatory potential on some human colon (HT29 and HCT116, liver (HepG2 and Huh7, breast (MCF-7 and MDA-MB-231 and prostate (DU145 cancer lines. Our results demonstrated that punicic acid and its congeners induce a significant decrease of cell viability for two breast cell lines with a related increase of the cell cycle G0/G1 phase respect to untreated cells. Moreover, the evaluation of a great panel of cytokines expressed by MCF-7 and MDA-MB-231 cells showed that the levels of VEGF and nine pro-inflammatory cytokines (IL-2, IL-6, IL-12, IL-17, IP-10, MIP-1α, MIP-1β, MCP-1 and TNF-α decreased in a dose dependent way with increasing amounts of the hydrophilic extracts of PSO, supporting the evidence of an anti-inflammatory effect. Taken together, the data herein suggest a potential synergistic cytotoxic, anti-inflammatory and anti-oxidant role of the polar compounds from PSO.

  2. Punica granatum (pomegranate) leaves extract induces apoptosis through mitochondrial intrinsic pathway and inhibits migration and invasion in non-small cell lung cancer in vitro.

    Li, Yali; Yang, Fangfang; Zheng, Weidong; Hu, Mingxing; Wang, Juanxiu; Ma, Sisi; Deng, Yuanle; Luo, Yi; Ye, Tinghong; Yin, Wenya

    2016-05-01

    Most conventional treatments on non-small cell lung carcinoma always accompany with awful side effects, and the incidence and mortality rates of this cancer are increasing rapidly worldwide. The objective of this study was to examine the anticancer effects of extract of Punica granatum (pomegranate) leaves extract (PLE) on the non-small cell lung carcinoma cell line A549, H1299 and mouse Lewis lung carcinoma cell line LL/2 in vitro, and explore its mechanisms of action. Our results have shown that PLE inhibited cell proliferation in non-small cell lung carcinoma cell line in a concentration- and time-dependent manner. Flow cytometry (FCM) assay showed that PLE affected H1299 cell survival by arresting cell cycle progression in G2/M phase in a dose-dependent manner and inducing apoptosis. Moreover, PLE could also decrease the reactive oxygen species (ROS) and the mitochondrial membrane potential (ΔYm), indicating that PLE may induce apoptosis via mitochondria-mediated apoptotic pathway. Furthermore, PLE blocked H1299 cell migration and invasion, and the reduction of matrix metalloproteinase (MMP) MMP-2 and MMP-9 expression were also observed in vitro. These results suggested that PLE could be an effective and safe chemotherapeutic agent in non-small cell lung carcinoma treatment by inhibiting proliferation, inducing apoptosis, cell cycle arrest and impairing cell migration and invasion. PMID:27133061

  3. Studies on the Chemical Constituents from Xinjiang Punica granatum%新疆石榴皮化学成分研究

    热娜·卡斯木; 帕丽达·阿不力孜; 张笑颖

    2009-01-01

    目的:研究新疆石榴皮(Punica granatum)的化学成分.方法:采用薄层色谱、硅胶柱色谱、MCI-gel CHP20P、Sephadex LH-20凝胶柱色谱等方法进行分离纯化,使用波谱分析技术和理化常数对照等方法对得到的化合物进行结构鉴定.结果:分离得到了10个化合物,分别为:没食子酸(1)、没食子甲酯(2)、鞣花酸(3)、(+)-儿茶素(4)、异槲皮苷(5)、D-甘露醇(6)、熊果酸(7)、齐墩果酸(8)、β-谷甾醇(9)、胡萝卜苷(10).结论:化合物1~10均、为首次从该植物中分离得到.

  4. 36个石榴(Punica granatum L.)品种的品质测定%Determination of Fruit Quality of 36 Pomegranate Cultivars

    汪小飞; 周耘峰; 赵昌恒; 孙龙; 伍全根

    2010-01-01

    对云南省蒙自县石榴品种园中来源于全国石榴产区的36个石榴(Punica granatum L.)品种,在相同栽培条件,相同树龄,均达到成熟的果实进行可溶性糖含量、有机酸含量、可溶性固形物含量、籽粒硬度等指标进行测定与分析.结果表明:'天红蛋'等29个石榴品种中达到优良,适合在蒙自栽培发展;'净皮甜'等9个品种适合作为水果鲜食类在当地栽培;'酸绿籽'等8个作为有机酸含量高的酸石榴品种,适合作为药用类在当地栽培.结果为当地石榴栽培品种的选择奠定了基础.

  5. Protection against oxidative damage in human erythrocytes and preliminary photosafety assessment of Punica granatum seed oil nanoemulsions entrapping polyphenol-rich ethyl acetate fraction.

    Baccarin, Thaisa; Mitjans, Montserrat; Lemos-Senna, Elenara; Vinardell, Maria Pilar

    2015-12-25

    The main purpose of the present study is to evaluate the ability of nanoemulsion entrapping pomegranate peel polyphenol-rich ethyl acetate fraction (EAF) prepared from pomegranate seed oil and medium chain triglyceride to protect human erythrocyte membrane from oxidative damage and to assess preliminary in vitro photosafety. In order to evaluate the phototoxic effect of nanoemulsions, human red blood cells (RBCs) are used as a biological model and the rate of haemolysis and photohaemolysis (5 J cm(-2) UVA) is assessed in vitro. The level of protection against oxidative damage caused by the peroxyl radical generator AAPH in human RBCs as well as its effects on bilayer membrane characteristics such as fluidity, protein profile and RBCs morphology are determined. EAF-loaded nanoemulsions do not promote haemolysis or photohaemolysis. Anisotropy measurements show that nanoemulsions significantly retrain the increase in membrane fluidity caused by AAPH. SDS-PAGE analysis reveals that AAPH induced degradation of membrane proteins, but that nanoemulsions reduce the extension of degradation. Scanning electron microscopy examinations corroborate the interaction between AAPH, nanoemulsions and the RBC membrane bilayer. Our work demonstrates that Punica granatum nanoemulsions are photosafe and protect RBCs against oxidative damage and possible disturbance of the lipid bilayer of biomembranes. Moreover it suggests that these nanoemulsions could be promising new topical products to reduce the effects of sunlight on skin. PMID:26407526

  6. Recent Advances in Metabolic Products of Flavonoids in Punica granatum%石榴类黄酮代谢产物的研究进展

    刘安成; 李慧; 王亮生; 庞长民; 卫伟光

    2011-01-01

    Pomegranate (Punica granatum) is a cultivated fruit with a long history and great nutritional value. China has 8 major pomegranate-producing areas, including Xingyang and Kaifeng cities, Henan province; Lintong county, Shaanxi province; Zaozhuang county, Shandong province; Huaiyuan county, Anhui province; Huili county, Sichuan province;Mengzi county, Yunnan province; and Kargilik county, Xinjiang Uyghur autonomous region. In total, 33 flavonoids have been detected from pomegranate fruit juice, pericarp and foliage; these include 6 flavanols, 1 flavanone glycoside, 2 flavonols, 3 flavonol glycosides, 3 flavones, 5 flavone glycosides, 1 flavanonol glycoside, 3 anthocyanidins and 9 anthocyanins. These flavonoid compounds are widely used in the health food and medicinal industries. This paper summarizes the recent progress in the constitution, content, detection methods and pharmacological studies of flavonoids in the fruit,leaf and petal of P. granatum. To promote deeper research and use of the active components and for functional characterization for cancer prevention, future study should focus on the rapid analysis, semi-preparation and/or preparation separation, and quantitative analysis of flavonoids in fruits; germplasm resource evaluation; and identification of active components in leaves and flowers of pomegranate.%石榴(Punica granatum)是一种栽培历史悠久且营养价值很高的水果,我国已形成了河南荥阳和开封、陕西临潼、山东枣庄、安徽怀远、四川会理、云南蒙自和新疆叶城8大石榴主产区.迄今为止,从石榴果汁、果皮和叶片中共检测出类黄酮多达33种,其中包括6种黄烷醇、1种黄烷酮苷、2种黄酮醇、3种黄酮醇苷、3种黄酮、5种黄酮苷、1种二氢黄酮醇苷、3种花青素苷元和9种花青素苷,这些化合物在保健食品和医疗应用中具有重要作用.该文对石榴果实、叶片和花瓣中所含类黄酮的组成、含量、检测方法及药理等

  7. Amelioration of radiation induced DNA damage and biochemical alterations by Punica Granatum (L) extracts and synthetic ellagic acid in Swiss albino mice

    Radiation therapy has been used in cancer treatment for many decades; Although effective in killing tumor cells, ROS produced in radiotherapy threaten the integrity and survival of surrounding normal cells. ROS are scavenged by radioprotectors before they can interact with biochemical molecules, thus reducing harmful effects of radiation. The pomegranate, Punica granatum L., an ancient, mystical, and highly distinctive fruit, is the predominant member of the Punicaceae family. It is used in several systems of medicine for a variety of ailments. The objective of the present study was to investigate the protective effects of ethanolic extracts of pomegranate whole fruit (EPWF) and seeds (EPS) and Synthetic Ellagic acid (EA) against Electron Beam Radiation (EBR) induced DNA damage and biochemical alterations in Swiss Albino mice. The extracts and synthetic compound were assessed for its radical scavenging property by DPPH radical scavenging and Ferric Reducing Antioxidant Power assays. The animals were treated with 200 mg/kg body wt. of pomegranate extracts and Ellagic acid for 15 days before exposure to 6 Gy of EBR. Radiation induced DNA damage was assessed by comet assay in the peripheral blood lymphocytes of mice. The biochemical estimations were carried out in the serum and RBC lysate of the animals. The plant extracts and synthetic compound exhibited good radical scavenging and reducing properties.The pretreated animals before irradiation caused a reduction in the comet length, olive tail moment, % DNA in tail when compared to irradiated group. The biochemical parameters such as lipid peroxidation was significantly depleted in the treated groups when compared to irradiated group followed by significant elevation in reduced glutathione. Our findings indicate the ameliorating effects of pomegranate extracts and synthetic ellagic acid on radiation induced DNA damage and biochemical changes in mice may be due to its free radical scavenging and increased antioxidant

  8. In vitro antioxidant, collagenase inhibition, and in vivo anti-wrinkle effects of combined formulation containing Punica granatum, Ginkgo biloba, Ficus carica, and Morus alba fruits extract

    Ghimeray AK

    2015-07-01

    Full Text Available Amal Kumar Ghimeray,1 Un Sun Jung,1,2 Ha Youn Lee,1 Young Hoon Kim,1 Eun Kyung Ryu,1 Moon Sik Chang11R&D Center, Natural Solution Co., Ltd, Gojan-dong, Namdong-gu, Incheon, Republic of Korea; 2Department of Horticultural Biotechnology, Kyung Hee University, Yongin, Republic of KoreaBackground: In phytotherapy, the therapeutic potential is based on the combined action of different herbal drugs. Our objective was to evaluate the antioxidant, anti-collagenase (in vitro, and anti-wrinkle (in vivo effect of combined formulation containing Ginkgo biloba, Punica granatum, Ficus carica, and Morus alba fruits extract.Methods: Antioxidant evaluation was based on the scavenging activity of free radicals (1,1-diphenyl-2-picrylhydrazyl, H2O2, and O2- and the anti-collagenase activity was based on the reduction of collagenase enzyme in vitro. In an in vivo study, 21 female subjects were examined in a placebo-controlled trail. Facial wrinkle, especially the crow's feet region of eyes, was treated with topical formulated 2% cream for 56 days and compared with the placebo.Results: In the in vitro study, the combination of fruits extract showed a higher antioxidant activity which was comparable with the positive standard (ascorbic acid, butylated hydroxyanisole, and Trolox. The data also showed a dose-dependent inhibition of collagenase. In the in vivo study, treatment with 2% formulated cream for 56 days significantly reduced the percentage of wrinkle depth, length, and area with 11.5, 10.07, and 29.55, respectively.Conclusion: The combined formulation of fruit extracts showed excellent antioxidative and anti-collagenase activity as well as a significant effect on anti-wrinkle activity on human skin.Keywords: antioxidant, anti-collagenase, anti-wrinkle, fruits, topical formulation

  9. 提高石榴坐果率的试验研究%Experimental Study on Improving the Fruit-set Percentage of Punica granatum

    王立忠

    2012-01-01

    Experiments were carried out on the cultivation techniques including artificial supplementary pollination in florescence and spray of gibberellin, etc., aimed to solve the problem of low fruit-set percentage of the soft-seed pomegranate (Punica granatum) in Huaibei City. The results showed that on the basis of intensive soil, fertilizer and water management and regular pruning, the fruit-set percentage could be increased by 31.7%~32.2%, 7.9%~22.3% and 51.3%~ 63.5% respectively with the application of artificial supplementary pollination in florescence, spray of gihberellin and their integrated application, compared with the control group. The optimal technical measures were figured out for maximum increase of the fruit-set percentage.%针对淮北软籽石榴坐果率低的问题,进行了花期人工辅助授粉和喷施赤霉素等栽培技术试验,结果表明:除加强土肥水管理和正常修剪外,花期采取人工辅助授粉、喷施赤霉素和授粉与喷施综合应用措施,其坐果率分别比对照提高31.7%~32.2%、7.9%~22.3%、51.3%~63.5%,摸索出提高坐果率的最佳技术措施。

  10. Avaliação da atividade antioxidante do extrato hidroalcoólico da romã (Punica granatum, L. sobre células da linhagem Caco-2 Antioxidant activity evaluation of the pomegranate (Punica granatum, L. hidroalcoholic extract at Caco-2 cell line

    Fernanda Archilla Jardini

    2007-08-01

    Full Text Available A absorção dos compostos antioxidantes presentes no extrato hidroalcoólico da polpa da romã (Punica granatum, L. foi avaliada utilizando-se o modelo de cultura de células, cuja linhagem escolhida foi a Caco-2, provenientes de um adenocarcinoma do cólon. O extrato hidroalcoólico da polpa apresentou um conteúdo de 832 µg equivalente de ácido gálico.mL-1 de extrato e sua atividade antioxidante mostrou valores de 5,9% (0,1 ppm a 93,09% (8 ppm de capacidade de redução do radical DPPH•. As células tratadas com o extrato apresentaram uma inibição de crescimento celular de 4,16% (200 ppm, e a absorção dos compostos antioxidantes foi de 63,25%. Para o ácido gálico, os resultados mostraram uma inibição de 2,98% (400 ppm e uma absorção de 72,54% dos compostos antioxidantes. Os resultados mostraram que o método de avaliação de absorção através de cultura de células foi eficaz, e que os compostos antioxidantes foram absorvidos pelas células, que se apresentaram viáveis, após um período de exposição prolongado aos compostos.The absorption of antioxidant compounds present in hydroalcoholic extract of pomegranate pulp (Punica granatum, L. was evaluated by a cell culture model, using the Caco-2 cells (from an adenocarcinom of the colon. The hydroalcoholic extract of the pulp showed a content of reducing compounds of approximately 832 µg equivalent to gallic acid.mL-1 of the extract and its antioxidant activity was from 5.9% (0.1 ppm to 93.09% (8 ppm, measured by the capacity of reducing the DPPH• radical. The cells that were treated with the extract showed a 4.16% of growing inibition (at a concentration of 200 ppm, and an absorption of the antioxidants compounds of approximately 63.25%. The gallic acid showed, at 400 ppm of concentration, a growing inhibition of 2.98% and anabsorption of 72.54% of the antioxidant compounds. These results pointed out that the method of evaluating the absorption by using cell culture was

  11. Controlling Biofilm Formation by Inhibiting the Quorum-Sensing Activity of Pseudomonas aeruginosa using the Ethanolic Extracts of Piper nigrum (Piperaceae Fruit, Punica granatum (Lythraceae Pericarp, and Pisum sativum (Fabaceae Seed

    M.V. Dazal

    2015-07-01

    Full Text Available Bacterial biofilm formation can cause serious problems in clinical and industrial settings, which drives the development or screening of biofilm inhibitors. Pseudomonas aeruginosa is a well-known pathogen that exhibit biofilm formation through quorum-sensing, which is a bacterial cell-to-cell communication that regulates the production of many virulence factors. The inhibition of biofilm formation is a viable option for bacterial eradication. The antibacterial effect of Piper nigrum is related to the presence of phenolic and flavonoid components. Punica granatum has been reported to possess a wide range of biological actions, with tannins and alkaloids stated to be the reason of its antibacterial property. Pisum sativum, on the other hand, contains various constituents, but the tannins and phenolic compounds stated as responsible for its antibacterial property. The minimum inhibitory concentration using the susceptibility testing of P. nigrum, P. granatum, P. sativum ethanolic extracts were 6.67×10-4 g/mL, 2.1978×10-5 g/mL, and 6.25×10-4 g/mL, respectively. On the swarming assay, P. granatum and P. sativum inhibits swarming motility at concentrations of 2.1978×10-2 up to 2.1978×10-4 g/mL, and 6.25×10-2 to 6.25×10-3 g/mL, respectively. The P. nigrum extract did not inhibit the motility.

  12. Study on the Effects of Different Methods on Punica granatum L.DNA Extraction from Leaves%不同方法对石榴叶片DNA提取效果的影响

    陈延惠; 张四普; 胡青霞; 栗燕; 阿依古丽·喀斯木; 朱道圩

    2005-01-01

    以4个石榴(Punica granatum L.)品种为实验材料,用SDSⅠ,SDSⅡ,CTABⅠ,CTABⅡ 4种方法提取石榴叶片DNA.结果表明,SDS法提取得率为500~600 μg·g-1,但OD值偏低,为1.50~1.70;RAPD分析无扩增;CTAB法所得DNA得率为200~500 μg·g-1,OD值为1.80~1.90;RAPD分析扩增带清晰.CTABⅠ和CTABⅡ相比,操作简单、省时,得率高,综合考虑CTABⅠ是石榴叶片DNA提取的最佳方法.

  13. Contribution à l'étude de i'activllé antioxydante et inhibitrice de l'a-amylase des proanthocyanidines extraites de l'épicarpe de Punica granatum L. (grenadier).

    bachiri, asma

    2015-01-01

    Le travail est réalisé dans le cadre d'évaluer les propriétés antioxydant et l'influence des proanthicyanidines extraites a partie de l'épicarpe de punica granatum sur l'hyperglycémie provoquée par voie orale(HGPO) ainsi que l'activité inhibitrice vis-à-vis de l'a amylase. Le pouvoir antioxydant de notre extrait est évalué par la méthode de FRAP (Ferric reducing antioxidantpower), et le résultat obtenu montre une activité très puissante pour réduire le fer, similaire avec celle...

  14. Preliminary Assessment of Punica granatum on Characteristics in Pupiao of Yunnan Province%云南蒲缥地方优良石榴品种白水籽和红水籽性状初步评价

    邹云昌

    2011-01-01

    通过对云南蒲缥地方石榴品种白水籽和红水籽的植物学性状、经济性状的观测,和与省内外16个优良石榴品种的比较,初步认为白水籽和红水籽品种已符合石榴良种指标,值得深入研究和开发。%Punica granatum,known as pomegranate,is a kind of fruit tree with high economic value.In this paper,comparative experiment of different varieties on their characteristics was carried out.Two local cultivars(with red meat and white meat respectively) are thought to be good enough to develop in Pupiao.

  15. 宾川石榴品种资源调查及其果实主要品质的比较分析%Investigation of Punica granatum L. Germplasm Resources in Binchuan and Comparative Analysis of Major Fruit Qualities

    陈黎虹; 袁责华; 李品兰; 王军; 杨黎静; 徐娟萍; 张波; 张庆辉; 杨荣萍

    2012-01-01

    [Objective] This study aimed to provide a basis for enriching the Punica granatum L. germplasm resources through investigating Punica granatum L. vari- eties in Binchuan. [Method] In this study, biological traits of Punica granatum L. vari- eties in Binchuan (including two local varieties-'Binchuan Red Coat' and 'Binchuan Green Coat', 'Cyan Coat and Soft Seeds' and 'Little Red Coat' introduced from Huili, Sichuan, 'Acid Pomegranate' introduced from Shandong Province and "Ma Pomegranate" from Fengqiu, Henan) were investigated and their major fruit qualities were also analyzed. [Result] "Cyan Coat and Soft Seeds' and "Ma Pomegranate' grew well with good fruit quality, which adapted well to the climate and environment in Binchuan; "Little Red Coat' showed average performance in growth and major fruit qualities with inferior flavour; "Binchuan Red Coat' and 'Binchuan Green Coat' also showed average performance in major fruit qualities and and inferior flavour; 'Acid Pomegranate' grew while was inferior in flavour. [Conclusion] This study pro- vides references for presenting the directions for studies of P. granatum L. germplasm resources and determining the genetic relationship among germplasm resources, as well as breeding, introduction and cultivation of P. granatum L., further providing a variety basis for pomegranate production.%[目的]调查研究宾川石榴品种资源,以期为扩大石榴品种资源奠定基础。[方法]对大理宾川的红皮石榴和绿皮石榴、以及引种的四川会理青皮软籽和小红皮、山东酸石榴、河南封丘麻石榴进行了生物学性状调查和果实主要品质分析。[结果]青皮软籽和麻石榴果树长势和果实主要品质较好,对宾川的气候环境有较强的适应性;而小红皮果树长势一般,果实主要品质一般,风味差;宾川本地红皮和绿皮石榴果树和果实主要品质一般,风味差;酸石榴虽然长势好,但风味差。[结论]该研究结果为

  16. Comparation of Pollen Ultrastructural Morphology Among Pomegranate (Punica granatum)Cultivars in Shandong Province%山东20个石榴品种花粉亚微形态学比较研究

    尹燕雷; 苑兆和; 冯立娟; 招雪晴; 陶吉寒

    2011-01-01

    The ultrastructures of pollen grains from 20 pomegranate (Punica granatum) cultivars in Shandong Province were examined using a Hitachi S-570 scanning electron microscope. The results showed that pollen grains were tricolporate, N3P4C5 type according to Erdtman's NPC system. They were sub-spheroidal in equatorial view, and 3-lobed sub-spheroidal or triangle in polar view. The polar axis length of pollen grains was 23.08- 28.57 μm. The equatorial axis length of pollen grains was 13.07-19.74 μm. In addition, there were significant differences in some morphological aspects of pollen grains,for example, pollen size and colporate, between cultivars. Thus, it was suggested that morphological characters of pollen grains were very important for cultivars differentiation in pomegranate.%利用日立S-570扫描电镜对山东省20个石榴(Punica granatum)品种花粉的亚微形态特征进行了观察分析,结果表明:供试品种的花粉具3条萌发沟,属N3P4C5类型;赤道面观均呈近长球形,极面观呈三裂圆形或三角形:花粉粒极轴长23.08~28.57μm,赤道轴宽13.07~19.74 μm;供试品种间花粉的大小、表面纹饰等均有明显差别.初步认为花粉粒的形态特征可以作为石榴品种间分类的一个标准.

  17. Genetic Diversity and Genetic Relationship of Pomegranate(Punica granatum)in Sichuan and Yunnan Evaluated by AFLP Markers%川滇石榴品种遗传多样性及亲缘关系的AFLP分析

    赵丽华; 李名扬; 王先磊

    2010-01-01

    @@ 石榴(Punica granatum)为石榴科(Punicaceae)石榴属(Punica)落叶灌木或小乔木,原产伊朗、阿富汗等中亚一带,从西汉张骞出使西域将涂林安石榴引入我国,石榴作为水果和观赏树木在我国已有2000多年的栽培历史.经长期天然杂交和基因突变,以及采用实生、分株、嫁接等多种繁殖方法,产生了复杂而多样的品种和类型,据不完全统计,我国现有石榴品种资源200多个,遍及南北各地20多个省区,形成了山东枣庄、新疆叶城、陕西临潼、安徽怀远、河南开封、云南蒙自和四川会理等著名的栽培区(汪小飞,2007).

  18. Photoprotection by Punica granatum seed oil nanoemulsion entrapping polyphenol-rich ethyl acetate fraction against UVB-induced DNA damage in human keratinocyte (HaCaT) cell line.

    Baccarin, Thaisa; Mitjans, Montserrat; Ramos, David; Lemos-Senna, Elenara; Vinardell, Maria Pilar

    2015-12-01

    There has been an increase in the use of botanicals as skin photoprotective agents. Pomegranate (Punica granatum L.) is well known for its high concentration of polyphenolic compounds and for its antioxidant and anti-inflammatory properties. The aim of this study was to analyze the photoprotection provided by P. granatum seed oil nanoemulsion entrapping the polyphenol-rich ethyl acetate fraction against UVB-induced DNA damage in the keratinocyte HaCaT cell line. For this purpose, HaCaT cells were pretreated for 1h with nanoemulsions in a serum-free medium and then irradiated with UVB (90-200 mJ/cm(2)) rays. Fluorescence microscopy analysis provided information about the cellular internalization of the nanodroplets. We also determined the in vitro SPF of the nanoemulsions and evaluated their phototoxicity using the 3T3 Neutral Red Uptake Phototoxicity Test. The nanoemulsions were able to protect the cells' DNA against UVB-induced damage in a concentration dependent manner. Nanodroplets were internalized by the cells but a higher proportion was detected along the cell membrane. The SPF obtained (~25) depended on the concentration of the ethyl acetate fraction and pomegranate seed oil in the nanoemulsion. The photoprotective formulations were classified as non-phototoxic. In conclusion, nanoemulsions entrapping the polyphenol-rich ethyl acetate fraction show potential for use as a sunscreen product. PMID:26406978

  19. Punica granatum – Granatapfelbaum (Punicaceae)

    Jagel, Armin

    2010-01-01

    Der Granatapfel ist bei uns zur Weihnachtszeit mittlerweile regelmäßig an Obstständen zu finden. Trotzdem gehört er für Mitteleuropäer noch immer zu den exotischen Früchten und oft weiß man nicht so recht, was man mit ihm anfangen soll. Ganz anders im Mittelmeergebiet. Hier wird der Granatapfel einerseits als Obstbaum, andererseits wegen seiner prachtvollen leuchtend roten Blüten häufig gepflanzt. Ihn umgibt eine Fülle von Symbolik und mythologischen Geschichten von der Antike bis ins Mittela...

  20. 河阴石榴茎尖脱毒快繁技术研究%The Research on the Fast Breeding and Virus-free Technologies of the Stem-tips of Punica granatum L. of Heyin

    范春丽

    2012-01-01

    The pomegranate explants from stem-tips were studied to obtain the optimum tissue culture system for punica granatum L. of heyin.The results showed that the most optimum multiplication medium was MS + 1.0 mg/L 6-BA + 0.30 mg/L NAA,whose multiplication index reached 4.20.The best rooting medium was 1 /2MS +0.50 mg/L NAA,whose rooting rate was 90.0%.The transplant survival rate could reach 96.0%.%以河阴石榴茎尖为材料,对其组织培养快繁体系进行了研究。结果表明,河阴石榴茎尖的最适增殖培养基为MS+1.0 mg/L 6-BA+0.30mg/L NAA,其增殖系数达4.20。最适宜生根培养基为1/2MS+0.50mg/L NAA,其生根率可达90.0%,移栽成活率达95.0%。

  1. Study of pomegranate (Punica granatum L.) peel extract containing anthocyanins on fatty streak formation in the renal arteries in hypercholesterolemic rabbits

    Fatemeh Sharifiyan; Ahmad Movahedian-Attar; Nafiseh Nili; Sedigheh Asgary

    2016-01-01

    Background: The influence of the supplementation of pomegranate peel extract containing anthocyanins on atherosclerotic plaque formation induced by hypercholesterolemia was investigated in renal arteries in rabbits. Materials and Methods: After the determination of polyphenol and anthocyanin′s content of P. granatum peel hydroalcoholic extract, 30 male rabbits were randomly divided into three groups. They were fed basic diet, hypercholesterolemic diet and hypercholesterolemic diet along w...

  2. 石榴皮鞣质对糖尿病大鼠血脂的影响及对肝脏的保护作用%Effects of Tannin in Punica granatum L.on Blood Lipids and Its Protecting Liver Ability in Diabetic Rats

    谭红军; 杨林; 罗春华; 张玉红

    2012-01-01

    Objective To study the effects of Tannin in Punica granatum L. on blood lipids and its protecting liver ability in diabeticrats. Methods Diabetic rat models were induced by i. p. injection of streptozotocin (75mg/kg). The diabetic rats were randomly divided into four groups;model group, tannin in punica granatum L. 50, 75, lOOmg/kg treatment Group. The blank control group was composed by normal rats. Serum alanine transaminase( ALT) activity of rats in each group, and glucose (GLU) , triglyceride ( TG) ,total cholesterol TC) ,high density lipoprotein - cholesterol ( HDL -C) , low density lipoprotein - cholesterol ( LDL C) levels were studied 21 days after medication administration. Results Compared with model Group, Tannin in punica granatum L. can lower GLU , TG,TC , LDL -C concentration and ALT activity significantly (P <0. 05 ,P <0. 01) , increase HDL - C levels (P < 0.05 ,P <0. 01). Conclusion Tannin in punica granatum L. has the function of good protection in liver of diabetic rats, It can lower blood glucose and regulate blood lipids.%目的 初步观察石榴皮鞣质对糖尿病大鼠血脂的影响及肝脏的保护作用.方法 采用腹腔注射链脲佐菌素(75mg/kg)的方法建立糖尿病大鼠模型,将成模大鼠随机分为4组:模型组、石榴皮鞣质低、中、高剂量组,另取正常大鼠作为空白对照组.连续给药21天后,观察石榴皮鞣质对血糖血脂和肝功能的影响.结果 与模型组比较,石榴皮鞣质可显著降低GLU、TG、TC、LDL-C浓度、ALT活性(P <0.05,P<0.01),提高HDL-C浓度(P <0.05,P<0.01).结论 石榴皮鞣质对糖尿病大鼠的肝脏具有保护作用,可降低血糖调节血脂.

  3. Effect of pectin methyl esterase and Ca²⁺ ions treatment on antioxidant capacity, shelf-life and quality of minimally processed pomegranate (Punica granatum L.) arils.

    Kumar, Sunil; Kumar, Ramesh; Nambi, V E

    2016-03-01

    Pomegranate fruits are difficult to peel and once peeled, extracted arils have very short shelf-life. Therefore, present investigation was carried out to extend the shelf life of minimally processed pomegranate arils using pectin methyl esterase (PME) and CaCl2 treatment during refrigerated storage. The arils of freshly harvested pomegranate fruits (Punica granatum L.) were treated with different concentrations of food-grade PME (50-300 units) and calcium ions (0.5-2.0% CaCl₂) for a period of 5-30 min using response surface methodology. Treated and untreated arils were then packed in low density polyethylene bags (25 μ) and maintained under low temperature (5°C; 90% RH) for evaluating the physical, biochemical and microbial quality of pomegranate arils at four day interval. Physiological loss in weight increased during storage but no food-borne pathogens were found during 28 day of cold storage in treated arils. Color and firmness of both treated and untreated arils decreased during storage but it was better maintained in treated arils. The firmness was found to be 0.630 N in treated samples compared to untreated one (0.511 N) after 20 d of storage. Total antioxidant capacity, ferric reducing antioxidant power, polyphenol oxidase and lipoxygenase activities increased during storage. Treatment with 249.33 units of PME and 1.70% CaCl₂for an immersion time of 24.93 min was found to be most effective treatment for maintaining the quality of minimally processed arils for longer period. Sensory score was also higher in treated pomegranate arils that were quite acceptable even after 20 day of referigerated storage as against 12 day for untreated ones. PMID:27097437

  4. 土壤水分对石榴光合速率的影响%Effect of soil water content on net photosynthetic rate of Punica granatum

    邓恒芳; 王克勤

    2005-01-01

    通过对不同土壤水分条件下石榴Punica granatum叶片光合速率的研究.结果表明,净光合速率和气孔导度都随着土壤含水量的减少而降低,二者的变化基本一致.净光合速率和气孔导度的日变化曲线都是典型的"双峰"型曲线,且随着土壤含水量的下降都有不同程度的下降.同时也表明土壤含水量为19.14%~13.23%时净光合速率的变化主要与气孔因素有关.图4表1参19

  5. 石榴皮鞣质对糖尿病大鼠血管保护作用及其机制初探%Study on Vascular Protection and Its Mechanism of Tannin in Punica Granatum L.in Diabetes Rats

    王明智; 梅志刚; 曾永保; 杨林; 谭红军

    2011-01-01

    目的 初步探讨石榴皮鞣质(tannin in punica granatum L.)对糖尿病大鼠血管保护作用及其机制.方法 采用腹腔注射链脲佐菌素(75mg/kg)的方法建立糖尿病大鼠模型,将成模大鼠随机分为三组:模型对照组、石榴皮鞣质低剂量组、石榴皮鞣质高剂量组,另取正常大鼠作为正常对照组.连续给药21天后,观察大鼠一般生理状况,测定血清葡萄糖(GLU)、三酰甘油(TG)、C反应蛋白(CRP)、一氧化氮(NO)、内皮素-1(ET-1)浓度以及NO/ET-1比值,并分析其相关性.结果 与模型对照组比较,低剂量的石榴皮鞣质可显著改善糖尿病大鼠生理状态,显著降低血清GLU、TG、CRP、ET-1浓度,显著提高血清NO浓度、NO/ET-1比值.单因素相关分析显示:血清GLU浓度与ET-1浓度呈显著正相关,与NO/ET-1比值呈显著负相关;血清TG浓度与ET-1浓度呈显著正相关,与NO/ET-1比值呈显著负相关.结论 石榴皮鞣质对糖尿病大鼠血管有较好的保护作用,其机制可能与其降低血糖、TG浓度,直接或间接的升高ET-1浓度、降低NO/ET-1比值有关.%Objective To study the vascular protection and its mechanism of tannin in punica granatum L in diahetes rats. Methods Diabetic rat models were induced by i. p. injection of streptozotocin (75mg/kg). The diabetic rats were randomly divided into three groups: model control group,tannin in punica granatum L low - dose group and high - dose group. Nurmal control group was composed by normal rats. Physiological state of of rats in each group, serum glucose. ( GLU) .triglyceride ( TG) , Creactive protein ( CRP) , NO, FT - 1 levels. NO/ET - 1 ratio and the correlation analysis were studied after 21 days medication administration. Results Compared with model control group, low dose of tannin in punica granatum L. can improve the physiological state of diabetic rats significantly, lower serum CRP. GLU, TC; and ET - 1 levels significantly, and inrrease serum NO level and NO/ET - 1

  6. 石榴皮多酚有效部位单次给药毒性及对无水乙醇致大鼠胃溃疡的保护作用%Single dose toxicity of polyphenols effective parts from Punica granatum and their protective effect on ethanol-induced gastric ulcer in rats

    史李娜; 王雪飞; 马桂芝; 康金森; 高晓黎

    2015-01-01

    上皮细胞合成、提高再生黏膜功能、增强抗氧化能力和诱导、促进NO合成有关。%Aims To study single dose toxicity of poly-phenols effective parts from Punica granatum,to eval-uate their safety,and thus to provide a theoretical basis for drug development and clinical use.To observe their protective effect on ethanol-induced gastric ulcer in rats.Methods 50 healthy Kunming mice were ran-domly divided into five groups and given different doses of polyphenols’effective parts from Punica granatum via intragastric administration.Toxicity and death in each group of mice were observed and recorded after administration for 14 d.The median lethal dose was calculated by Bliss method.70 rats were randomly di-vided into normal group,model group(constant volume of normal saline),sanjiuweitai particles(1 850 mg· kg-1 )group,colloidal bismuth subcitrate (33 mg · kg-1 )group and polyphenols effective parts from Puni-ca granatum low-dose,medium-dose,high-dose(430, 852,1 704 mg·kg-1 )groups.On the 9th day of 10 days’gavage,all except the normal group were fed ethanol (1.5 mL/only)to induce gastric mucosal inju-ry in rats with acute gastric ulcer.Gastric ulcer index, the rate of ulcer inhibition were calculated for each group.The morphological changes of gastric mucosa were observed.The gastric mucosa levels of PGE2 , NO,SOD and MDA were determined.Results The LD50 and 95%confidence limit of the polyphenols’ef-fective parts from Punica granatum were 8 520.9 mg· kg-1 and 7 291.2 ~9 914.4 mg·kg-1,respectively. Pathology showed that the organs receiving dose of 16 000 mg · kg-1 had different degrees of damage . Compared with the model group,the extract from Puni-ca granatum significantly repaired the gastric mucosa, and significantly increased the gastric mucosa levels of NO and reduced MDA content,and improved SOD content and the levels of PGE2 .Conclousion The dose of 5 063 mg · kg-1 of polyphenols effective part from Punica granatum showed no

  7. HPLC测定石榴皮提取物颗粒剂中不同时期鞣花酸含量的变化%Detection of Ellagic Acid in the Granules of Extracts of Punica granatum L. Pericarp in Different Periods by HPLC

    任杰; 姚敬明; 武果桃; 樊振华; 赵娟; 吴忻; 刘文俊; 孟冬霞

    2012-01-01

    The ellagic acid in the granules of extracts of Punica granatum L. pericarp in different periods was detected by HPLC. Chromatography was performed on a PT -230A Colume heater Apollo C18 column (150 mm×4.6 mm, 5 μm) at 254 nm and 30℃. The mobile phase consisted of a mixture of acetonitrile - 1.2% phosphoric acid (20 : 80) at a flow rate of 1.0 mL/min. The linear range of ellagic acid was 0.52 -5.20 txg with (R2 =0. 99786). The average recovery was 97.8% and RSD was 1.6%. The samples of March 25, 2008 and March 25, 2011 were 0. 495 mg/g, 0. 439 mg/g. The results showed that the granules of extracts of Punica granatum L. pericarp was stable within three years. The method is simple and accurate and reproducibe for quality control of Punica granatum L. pericarp.%采用高效液相色谱法测定了石榴皮提取物颗粒剂在不同时期的有效成分鞣花酸含量的变化。色谱柱PT-230A Colume heater Apollo C18柱(150 mm×4.6 mm,5μm),柱温30℃;流动相为乙腈-1.2%磷酸溶液(20∶80);检测波长254 nm;流速1.0 mL/min。结果显示,鞣花酸含量测定的线性范围0.52~5.20μg(R2=0.99786),平均加样回收率为97.8%,RSD为1.6%。样品于2008年3月25日测定的颗粒剂鞣花酸含量为0.495 mg/g,2011年3月25日测定的含量为0.439 mg/g。表明该石榴皮提取物颗粒剂稳定性良好,有效期可达到三年以上。该方法简便、准确,可用于中药石榴皮的质量控制。

  8. 石榴皮鞣质对腺嘌呤性慢性肾衰大鼠保护作用的研究%Protective Effect of Tannin in Punica granatum L.on Chronic Renal Failure in Rats Induced by Adenine

    周本宏; 郭志磊; 王慧媛; 冯琪

    2009-01-01

    目的 观察石榴皮鞣质对腺嘌呤性慢性肾功能衰竭(CRF)大鼠的影响.方法 用250 mg·kg~(-1)腺嘌呤溶液连续灌胃21 d使Wistar大鼠产生类似慢性肾袁的症状.21d后,治疗组大鼠每天用石榴皮鞣质(40,20,10mg·kg~(-1).d~(-1))灌胃,阳性组用20%尿毒清混悬液按2.1 g·kg~(-1)·灌胃.60 d后收集尿液,处死大鼠,取血、留取肾组织,测定血Ca~(2+)、P~(3+)及血肌酐(Scr)、尿素氮(BUN),血脂和24h尿蛋白定量、尿Ca~(2+)、P~(3+),进行统计学处理,并进行形态学观察.结果 石榴皮鞣质对慢性肾衰竭大鼠的血Ca~(2+)、P~(3+)及Scr、BUN和24 h尿蛋白定量、尿Ca~(2+)、P~(3+)有不同程度的改善.结论 石榴皮鞣质对腺嘌呤导致的大鼠慢性肾衰竭有一定的保护作用.%OBJECTIVE To observe the effeets of tannin in Punica granatum L.on chronic renal failure(CRF)in rats induced by adenine.METHODS The Wistar rats were administered intragastrically with adenine(200 mg·kg~(-1)·d~(-1))for 21 d to induce the symptoms similar to chronic renal failure.Then,the rats were administered intragastrically with tannin(40,20 and 10 blood urea nitrogen(BUN),calcium ion(Ca~(2+)),phosphorus ion(P~(3+)),blood lipids and urine protein of 24 h were determined and analyzed.Meanwhile morphology was observed.RESULTS Serum levels of Scr,BUN and P~(3+) were decreased but Ca~(2+) level was increased by tannin in Punica granatum L.CONCLUSION Tannin in Punica granatum L can improve the renal function injuried by metabolic products in adenine-modeled CRF rats.

  9. Changes of Photosynthetic Capability and Pigment Contents of Malus spectabilis,Punica granatum and Amygdalus persica Under Salt Stress%盐胁迫对海棠等3树种光合能力及色素变化的影响

    孙方行; 周勃; 孙明高

    2010-01-01

    以2年生海棠(Malus spectabilis)、石榴(Punica granatum)和桃树(Amygdalus persica)的盆栽苗为试验材料,测定它们在不同浓度NaCl胁迫下的光合能力及色素变化.结果表明,3树种的耐盐性依次为海棠>石榴>桃树;盐胁迫下3种非盐生树种净光合速率(Pn)和气孔导度(Gs)下降,胞内CO2浓度(Ci)变化没有规律,非气孔因素是导致光合下降的限制因子;盐胁迫导致3树种叶绿素含量下降,但叶绿素a/b的变化各不相同.

  10. Effect of S. macrosiphon and L. perfoliatum seed gums on rheological characterization of bitter orange (Citrus aurantium L.) and pomegranate (Punica granatum L.) paste blends.

    Asnaashari, Maryam; Motamedzadegan, Ali; Farahmandfar, Reza; Rad, Tandis Khosravi

    2016-02-01

    The steady shear flow properties of bitter orange and pomegranate pastes and blend of two pastes including 0.5% Salvia macrosiphon (SMG) and L. perfoliatum (LPG) seed gums, two traditional Iranian hydrocolloids, were determined. All treatments exhibited shear-thinning behaviour. LPG added sample showed stronger shear thinning behaviour than the other due to its high molecular weight and intermolecular through hydrogen bonds and polymer entanglement. Ostwald model was found the best model to describe steady shear flow behaviour among different time-independent rheological model applied. Treatments including 0.5% these two seed gums indicated a flow behaviour index less than 0.6 and consistency coefficient raised by increasing concentration from 60 °Bx to 76 °Bx in bitter orange paste (from 0.55 Pa s(n) to 32.58 Pa s(n)), pomegranate paste (from 0.55 Pa s(n) to 84.87 Pa s(n)) and mix of these two pastes (from 0.64 Pa s(n) to 56.9 Pa s(n)). Oscillatory shear data showed weak gel-like behaviour of bitter orange and pomegranate pastes treatments including seed gums with the elastic modulus predominating over the viscous one at lower frequency. However, after weak gel formation, G″ was higher than G' in the frequency range of 0.01 to 10 Hz. An Ostwald model was used to describe the changes of viscose modulus with frequency. The results indicate that the elastic properties of bitter orange/ pomegranate paste and bitter orange paste may be increased by the presence of LPG and SMG due to associations of ordered chain segments of these gums, resulting in a weak three-dimensional network. PMID:27162409

  11. Antibacterial Activity of Punica granatum Linn.

    Kenan Tunç

    2013-08-01

    Full Text Available In this study, it was investigated that the extracts (ethanol, aceton, methanol, ethyl acetat obtained from fruit peels of the plant to whether has antibacterial effect against Streptococcus mitis CNCTC 4/77, Streptococcus salivarius CNCTC 64/59, Streptococcus mutans CNCTC 8/77, Staphylococcus epidermidis ATCC 12228, Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, Salmonella abony NCTC 6017, Salmonella typhimurium ATCC 14028, Enterococcus faecalis ATCC 29212, Bacillus subtilis ATCC 6633 bacteria strains in vitro. The antibacterial activity of extracts was evaluated according to disc diffusion method. It has been determined that pomegranate peel's extract had the highest inhibition zone diameters ( 18-30 mm against S. epidermidis and S. aureus bacteria strains. The antibacterial activity of plant against Streptococcus mitis is determined in this study for the first time.

  12. Antioxidant activity directed isolations form punica granatum

    The extracts derived from pomegranate juice following antioxidant activity directed isolation were screened for their antioxidant activity through their ability to scavenge 2,2- diphenyl-l-picrylhydrazyl (DPPH) radicals. Only fractions which exhibited >50/0 DPPH scavenging effect at each step of isolation were selected for further purification and their ability to reduce peroxide formation (peroxide value) in heated com oil. Phytochemical analysis of the pure compounds finally obtained, revealed the presence of pelargonidin-3- galactose (Pg-3-galactose), cyanidin-3-glucose (Cy-3-Glucose), gallic acid, quercetin and myricetin in the fractions exhibiting >50% DPPH scavenging potential. The order of antioxidant activity of these pure compounds by DPPH method was found to be gallic acid> quercetin> myricetin> Cy-3-galactose> Pg-3-Glucose while order with respect to reduction in peroxide value (PV) was the reverse of DPPH. (author)

  13. Estudo da ação antimicrobiana conjunta de extratos aquosos de Tansagem (Plantago major l., Plantaginaceae e Romã (Punica granatum l., Punicaceae e interferência dos mesmos na ação da amoxicilina in vitro

    D.G. Teles

    2014-01-01

    Full Text Available O uso de plantas medicinais no tratamento de doenças (fitoterapia é uma prática de diversos povos, sendo realizada desde a Antiguidade. Diversos estudos são realizados para determinar a eficácia dessas plantas, sendo desconhecida a existência de interações e interferências na combinação das mesmas, quer seja benéfica, quer cause algum dano para o organismo. A fim de descobrir a existência dessas interações ou a interferência na ação do antibiótico amoxicilina testou-se Punica granatum e Plantago major contra as bactérias Staphylococcus aureus e Escherichia coli. A matéria prima foi obtida nas regiões de Montes Claros e Curvelo e seus extratos aquosos foi obtido através da decocção. A Concentração Inibitória Mínima (CIM dos extratos foi determinada pela técnica de microdiluição em caldo. Para o teste de interferência realizou-se o teste de disco-difusão. No teste de microdiluição em caldo, Punica granatum apresentou CIM de 6,25% contra o S. aureus e de 25% contra E. coli. O extrato de Plantago major não apresentou atividade contra as bactérias. O teste de disco-difusão confirmou a existência de interações, sendo apenas quatro resultados significativos (Amoxicilina + Plantago major Pura (PgP, Amoxicilina + Plantago major Diluição 1 (PgD1 e Amoxicilia + Plantago major Diluição 2 (PgD2 contra E. coli e Amoxicilina + PgP contra S. aureus. Conclui-se que existe interação dos extratos entre si e dos extratos com a amoxicilina, o que também pode acontecer com outros antibióticos e gerar riscos para a população. Portanto é indispensável à realização de testes que complementem essa pesquisa oferecendo mais informação à população para segurança na utilização de plantas medicinais. informações à população assegurando sua segurança na utilização de plantas medicinais.

  14. Medicinal Uses of Punica Granatum And Its Health Benefits

    Debjit Bhowmik; Harish Gopinath; B. Pragati Kumar; S.Duraivel; Aravind. G; K.P. Sampath Kumar

    2013-01-01

    Pomegranate has been used for thousands of years to cure a wide range of diseases across different cultures and civilizations. It has great nutritional values and numerous health benefits. Pomegranates as a Treatment for Cancer, Osteoarthritis and Other Diseases. The pomegranate has been used in natural and holistic medicine to treat sore throats, coughs, urinary infections, digestive disorders, skin disorders, arthritis, and to expel tapeworms. However, modern research suggests that pomegran...

  15. Root activity distribution pattern of Ganesh pomegranate (Punica granatum) seedlings

    In one-year old Ganesh pomegranate seedlings raised on a loamy sand (Typic Haplustalf) under rain fed conditions, during winter (January-March) one-half of the active roots (44-51%) resided at 50 cm radial distance. Depth wise, bulk (44-78%) of the roots were found at 15 cm depth. The results indicate that the zone of high root activity is located around 50 cm distance from trunk and applications of fertilizers in this zone may lead to enhanced use efficiency of fertilizers. (author)

  16. Medicinal Uses of Punica Granatum And Its Health Benefits

    Debjit Bhowmik

    2013-01-01

    Full Text Available Pomegranate has been used for thousands of years to cure a wide range of diseases across different cultures and civilizations. It has great nutritional values and numerous health benefits. Pomegranates as a Treatment for Cancer, Osteoarthritis and Other Diseases. The pomegranate has been used in natural and holistic medicine to treat sore throats, coughs, urinary infections, digestive disorders, skin disorders, arthritis, and to expel tapeworms. However, modern research suggests that pomegranates might be useful in treating such serious conditions as prostate cancer, skin cancer, osteoarthritis, and diabetes. Studies also show that pomegranate seeds might help rid the digestive system of fats. Clinical research shows that pomegranates, when part of a healthy diet, might help prevent heart disease, heart attacks and strokes. This is because pomegranates have the potential to thin the blood, increase blood flow to the heart, reduce blood pressure, reduce plaque in the arteries, and reduce bad cholesterol while increasing good cholesterol. A decoction of seed is used to treat syphilis. Juice used to treat jaundice and diarrhoea. Juice of flower is used to treat nose bleeds. The fruit pulp and the seed are stomachic. Dried, pulverized flower buds are employed as a remedy for bronchitis.

  17. Ellagitannins of the fruit rind of pomegranate (Punica granatutm) antagonize in vitro the host inflammatory response mechanisms involved in the onset of malaria

    Bhattacharya Deepak; Romeo Sergio; Basilico Nicoletta; Bulgari Michela; Galli Germana V; Dell'Agli Mario; Taramelli Donatella; Bosisio Enrica

    2010-01-01

    Abstract Background The sun-dried rind of the immature fruit of pomegranate (Punica granatum) is presently used as a herbal formulation (OMARIA, Orissa Malaria Research Indigenous Attempt) in Orissa, India, for the therapy and prophylaxis of malaria. The pathogenesis of cerebral malaria, a complication of the infection by Plasmodium falciparum, is an inflammatory cytokine-driven disease associated to an up-regulation and activity of metalloproteinase-9 and to the increase of TNF production. T...

  18. КЛИНИКО-ЛАБОРАТОРНАЯ ОЦЕНКА ЭФФЕКТИВНОСТИ ГРАНАТОВОГО МАСЛА (PUNICA GRANATUM L.) В ЛЕЧЕНИИ ВОСПАЛИТЕЛЬНЫХ ЗАБОЛЕВАНИЙ ПАРОДОНТА

    ГАРАЕВ З.И.; АЛИЕВ А. А.

    2014-01-01

    В выборе лечебных манипуляций при воспалительных заболеваниях пародонта наиболее часто применяется профессиональная гигиена полости рта. При этом по мере отягощения воспалительного процесса в тканях пародонта наблюдается тенденция к снижению частоты ее применения. Хирургическое лечение чаще проводится при диагностировании пародонтита тяжелой степени. По результатам клинических и лабораторных исследований доказано выраженное противовоспалительное действие гранатового масла «Punica granatum». П...

  19. Pomegranate juice (Punica granatum as an ideal mouthrinse for fixed orthodontic patients

    Haryono Utomo

    2012-12-01

    Full Text Available Background: Prevention of caries as well as periodontal disease is mandatory during orthodontic treatment. Nevertheless, the use of antiseptic mouthrinse is contraindicated for prolonged use. Pomegranate juice is a polyphenol-rich juice with high antioxidant capacity as well as antimicrobial properties. It has been shown to exert beneficial characteristics for orthodontic patients such as antioxidant and anti inflammatory effects. Moreover, it contained fluoride and phosphorous which are cariostatic. Previous study in fixed orthodontic patients revealed that rinsing with this juice showed reduced dental plaque and superior compared with chlorhexidine. If it has unwanted effect by reducing pro-inflammatory reaction that also needed in orthodontic movement is not clearly understood. Purpose: The aim of the present review was to discuss the beneficial and unwanted effect of pomegranate juice mouthrinse towards orthodontic treatment. Reviews: Pomegranate has antimicrobial activity, its methanolic skin extract is the most potent followed by seed juice. Nevertheless, seed juice is not only tastier, easier to make but also has mild antimicrobial potency which is beneficial for long-term use. Healthy periodontal tissue is preferable for orthodontic movement since it resulted in less unwanted bone resorption. Conclusion: Regarding its beneficial effect and safety of pomegranate juice if use daily mouthrinse in fixed orthodontic patients, it could be proposed as an ideal long term use mouthrinse for fixed orthodontic patients. However, further researches should be done to verify this concept.Latar belakang: Pencegahan karies dan penyakit periodontal sangat penting dalam perawatan ortodontik. Walaupun demikian, penggunaan obat kumur antiseptik jangka panjang merupakan kontraindikasi. Jus buah delima sangat kaya akan polifenol dengan kemampuan antioksidan yang tinggi disertai kemampuan antimikroba. Beberapa penelitian telah menunjukkan dampak menguntungkan pada pasien ortodonti, yaitu sebagai antioksidan dan anti radang. Selain itu juga mengandung fluor dan fosfor yang bersifat kariostatik. Penelitian terdahulu pada pasien ortodonti cekat menunjukkan penurunan plak gigi yang lebih banyak dibanding dengan klorheksidin. Akan tetapi, apakah juga menyebabkan dampak merugikan yaitu mengurangi reaksi keradangan yang juga penting bagi pergerakan ortodonti masih belum jelas. Tujuan: Membahas dampak menguntungkan maupun merugikan jus buah delima pada perawatan ortodonti. Tinjauan pustaka: Buah delima merupakan antimikroba, ekstrak dari kulit yang mengandung metanol adalah paling kuat diikuti jus biji delima. Akan tetapi, jus biji lebih enak rasanya, mudah dibuat dan merupakan antimikroba ringan sehingga menguntungkan untuk pemakaian jangka panjang. Jaringan periodontal yang sehat diperlukan untuk pergerakan ortodonti karena dapat mengurangi resorpsi tulang. Kesimpulan: Berdasarkan dampak menguntungkan dan keamanan jus buah delima bila digunakan tiap hari, bahan ini dapat diusulkan sebagai obat kumur jangka panjang yang ideal untuk pasien ortodonti cekat. Walaupun begitu, penelitian lebih lanjut harus dilakukan untuk verifikasi konsep ini.

  20. In vitro propagation of pomegranate (Punica granatum l.) Cv. ‘Males Yazdi’

    Babak Valizadeh Kaji; Ahmad Ershadi; Masoud Tohidfar

    2013-01-01

    Conventional method of propagation of pomegranate is time consuming and tiresome, and it does not ensure disease free and healthy plants. In vitro technique is the only prospect of plant tissue culture that has the potential to circumvent these problems. An efficient in vitro propagation is described for pomegranate using shoot tips and nodal explants. The influence of two basal medium, WPM and MS, and different plant growth regulators was investigated on micropropagation of the Iranian culti...

  1. Pomegranate juice (punica granatum): a new storage medium for avulsed teeth.

    Sara Tavassoli-Hojjati; Elham Aliasghar; Fatemeh Ahmadian Babaki; Fatemeh Emadi; Maliheh Parsa; Shohreh Tavajohi; Maryam Ahmadyar; Seyed Nasser Ostad

    2014-01-01

    Objective There is evidence indicating that pomegranate juice contains many of the essential properties necessary to retain cell viability and cell proliferation. These properties indicate that pomegranate juice is a suitable storage medium for avulsed teeth. However, this idea has not yet been tested. In this study, the capacity of pomegranate juice (PJ) as a storage medium for retaining avulsed teeth was evaluated. Materials and Methods: PDL fibroblasts were obtained from healthy human prem...

  2. Correlation between the morphological characters of pomegranate (Punica granatum) traits and their implications for breeding

    Karimi, Hamid Reza; MIRDEHGHAN, Seyed Hossein

    2013-01-01

    Pomegranate is one of the most important horticultural crops in Iran. Selection of suitable genotypes resistant to unfavourable environmental and soil conditions and diseases is important for increasing the yield efficiency and acreage of this important crop. The aim of this research was to compare commercial pomegranate genotypes in Iran and to determine any correlations between morphological characteristics. Eight pomegranate types were used during the research. Twenty-six morphological cha...

  3. Optimization of ultrasonic-assisted extraction of pomegranate (Punica granatum L.) seed oil.

    Tian, Yuting; Xu, Zhenbo; Zheng, Baodong; Martin Lo, Y

    2013-01-01

    The effectiveness of ultrasonic-assisted extraction (UAE) of pomegranate seed oil (PSO) was evaluated using a variety of solvents. Petroleum ether was the most effective for oil extraction, followed by n-hexane, ethyl acetate, diethyl ether, acetone, and isopropanol. Several variables, such as ultrasonic power, extraction temperature, extraction time, and the ratio of solvent volume and seed weight (S/S ratio) were studied for optimization using response surface methodology (RSM). The highest oil yield, 25.11% (w/w), was obtained using petroleum ether under optimal conditions for ultrasonic power, extraction temperature, extraction time, and S/S ratio at 140 W, 40 °C, 36 min, and 10 ml/g, respectively. The PSO yield extracted by UAE was significantly higher than by using Soxhlet extraction (SE; 20.50%) and supercriti cal fluid extraction (SFE; 15.72%). The fatty acid compositions were significantly different among the PSO extracted by Soxhlet extraction, SFE, and UAE, with punicic acid (>65%) being the most dominant using UAE. PMID:22964031

  4. Characterization of constituents, quality and stability of pomegranate seed oil (Punica granatum L.

    Illana Louise Pereira de MELO

    2016-03-01

    Full Text Available Abstract This study aimed to characterize pomegranate seed oil and evaluate its quality and stability parameters against those of linseed oil. The profile of fatty acids and phytosterols and the content of tocopherols were analyzed by gas chromatography and high performance liquid chromatography, respectively. The quality of both oils was assessed as recommended by the American Oil Chemists' Society (AOCS and stability was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH, β-carotene bleaching (coupled oxidation of β-carotene/linoleic acid and Rancimat® assays. While α-linolenic acid (52% was the most abundant fatty acid in linseed oil (LO, punicic acid (55% was highest in pomegranate seed oil (PSO. Tocopherols and phytosterols (175 and 539 mg/100 g, respectively were greater in PSO than in LO (51 and 328 mg/100 g, respectively. Both oils met quality standards. The β-carotene bleaching and the DPPH assays showed greater oxidative stability for PSO than for LO. The Rancimat® method, on the other hand, indicated low stability for both oils.

  5. Comparative proteomics analysis of pomegranate seeds on fruit maturation period (Punica granatumL.)

    CAO Shang-yin; NIU Juan; CAO Da; LI Hao-xian; XUE Hui; CHEN Li-na; ZHANG Fu-hong; ZHAO Di-guang

    2015-01-01

    Seeds play a central role in the life cycle of plants. Seed hardness in pomegranates is of economic relevance, yet scarcely studied and poorly understood in China. In this study, we compared the proteomic differences between Zhong-nonghong (soft-seeded) and Sanbai (hard-seeded) pomegranates. A total of 892 protein spots from both varieties were detected on two-dimensional electrophoresis gels (2-DE); 76 spots showed greater than a 1.5-fold or less than a 0.66-fold difference (P<0.05) in Zhongnonghong compared to Sanbai, of which 24 exhibited greater than a 2-fold change. Compared with Sanbai, Zhongnonghong possessed 14 up-regulated, and 10 down-regulated proteins. We identiifed and annotated 5 of these by using MALDI-TOF-TOF MS: pyruvate dehydrogenase (PDH) E1-β family protein (spot 4609); alanine aminotransferase 2-like (ALT2L); mitochondrial glycine decarboxylase complex P-protein (spot 5803);phosphofructokinase B (PfkB)-type family of carbohydrate kinase (spot 8411); and putative dnaK-type molecular chaperone heat shock cognate protein 70 (Hsc70) (spot 9006). Of these, 3 proteins (spots 4609, 5608, 5803) were hypothesized to play a role in the formation of seed hardness. The other two proteins (spots 8411, 9006) were theo-rized to play a role in protecting the seeds from adverse stress during periods of fruit maturation. This study sets the foundation for further research on molecular mechanisms related to pomegranate seed hardness.

  6. CALLUS INDUCTION AND PLANT REGENERATION IN PUNICA GRANATUM L. ?NANA' FROM LEAF EXPLANTS

    Alireza Bonyanpour

    2013-09-01

    Full Text Available ABSTRACT In this investigation, leaf explants of a local cultivar of dwarf pomegranate were placed on Murashige and Skoog (1962 (MS medium supplemented with various concentrations of 6-benzyl adenin (BA and naphthalene acetic acid (NAA for callus induction. After 40 days, maximum callus induction was observed on a media containing 1 mg L-1 BA and 0.2 to 0.4 mg L-1 NAA. However, the highest callus growth was obtained on a medium containing 1 mg L-1 BA and 1 mg L-1 NAA. The highest number of shoots (7 shoots per explants was obtained by transferring the calli to the media containing 5 mg L-1 BA with 0.1 mg L-1 NAA. Maximum shoot proliferation was observed when shoots were cultured on woody plant medium (WPM supplemented with 5 mg L-1 kinetin (Kin. In this treatment, after 4 subcultures, 36 shoots were produced from one original explant. Among treatments used in rooting experiments, shoots cultured on WPM medium containing 0.2 mg L-1 indol butyric acid (IBA had the maximum root percentage (100% and good root growth (2.06 cm mean length and 2 roots in each explants. Rooted plantlets were cultured in a soil mixture containing vermiculite (60%, perlite (30% and coco peat (10% v/v. After 2 months, 80% of plants survived and transferred to the greenhouse.

  7. Evaluation of antioxidant and antiradical properties of Pomegranate (Punica granatum L.) seed and defatted seed extracts

    Basiri, Shadi

    2013-01-01

    Pomegranate seeds are byproducts of the Pomegranate juice industries that contains functional compounds such as phenols. This study was aimed to evaluate the effect of solvents on extraction from Pomegranate seed and Pomegranate defatted seed and to measure the yield extract and phenolic content and antioxidant properties. For this purpose, the seeds and defatted seeds were directly isolated from fruits and seeds by cold pressing respectively, then were crushed and extracted with different so...

  8. Spatial distribution of root activity of Ganesh pomegranate (Punica granatum) plants

    Information on the root activity pattern is invaluable while adopting appropriate time and method of fertilizer application, irrigation, planting distance and other cultural practices. The pattern of root activity distribution determined using the technique of soil injection of 32P compares well with actual pattern ascertained by root excavation method. The isotopic technique is also non-destructive, quick and inexpensive. Root activity distribution pattern has been determined using this technique in citrus, grape, mango, guava and papaya. To generate the information on inbred Ganesh pomegranate seedlings, studies were undertaken and the results are reported in this paper. (author)

  9. Molecular Polymorphisms in Tunisian Pomegranate (Punica granatum L. as Revealed by RAPD Fingerprints

    Jemni Chibani

    2010-01-01

    Full Text Available The genetic diversity among Tunisian pomegranate cultivars has been investigated. Using universal primers, the random amplified polymorphic DNA (RAPD method was used to generate banding profiles from a set of twelve cultivars. Data was then computed with appropriate programs to construct a dendrogram illustrating the relationships between the studied cultivars. Our data proved the efficiency of the designed method to examine the DNA polymorphism in this crop since the tested primers are characterized by a collective resolving power of 12.83. In addition, the cluster analysis has exhibited a parsimonious tree branching independent from the geographic origin of the cultivars. In spite of the relatively low number of primers and cultivars, RAPD constitutes an appropriate procedure to assess the genetic diversity and to survey the phylogenetic relationships in this crop.

  10. CALLUS INDUCTION AND PLANT REGENERATION IN PUNICA GRANATUM L. ?NANA' FROM LEAF EXPLANTS

    Alireza Bonyanpour; Morteza Khosh-Khui

    2013-01-01

    ABSTRACT In this investigation, leaf explants of a local cultivar of dwarf pomegranate were placed on Murashige and Skoog (1962) (MS) medium supplemented with various concentrations of 6-benzyl adenin (BA) and naphthalene acetic acid (NAA) for callus induction. After 40 days, maximum callus induction was observed on a media containing 1 mg L-1 BA and 0.2 to 0.4 mg L-1 NAA. However, the highest callus growth was obtained on a medium containing 1 mg L-1 BA and 1 mg L-1 NAA. The highest number o...

  11. Bioactive properties of commercialised pomegranate (Punica granatum) juice: antioxidant, antiproliferative and enzyme inhibiting activities.

    Les, Francisco; Prieto, Jose M; Arbonés-Mainar, Jose Miguel; Valero, Marta Sofía; López, Víctor

    2015-06-01

    Pomegranate juice and related products have long been used either in traditional medicine or as nutritional supplements claiming beneficial effects. Although there are several studies on this food plant, only a few studies have been performed with pomegranate juice or marketed products. The aim of this work is to evaluate the antioxidant effects of pomegranate juice on cellular models using hydrogen peroxide as an oxidizing agent or DPPH and superoxide radicals in cell free systems. The antiproliferative effects of the juice were measured on HeLa and PC-3 cells by the MTT assay and pharmacologically relevant enzymes (cyclooxygenases, xanthine oxidase, acetylcholinesterase and monoamine oxidase A) were selected for enzymatic inhibition assays. Pomegranate juice showed significant protective effects against hydrogen peroxide induced toxicity in the Artemia salina and HepG2 models; these effects may be attributed to radical scavenging properties of pomegranate as the juice was able to reduce DPPH and superoxide radicals. Moderate antiproliferative activities in HeLa and PC-3 cancer cells were observed. However, pomegranate juice was also able to inhibit COX-2 and MAO-A enzymes. This study reveals some mechanisms by which pomegranate juice may have interesting and beneficial effects in human health. PMID:26030005

  12. Molecular Polymorphisms in Tunisian Pomegranate (Punica granatum L.) as Revealed by RAPD Fingerprints

    Jemni Chibani; Mokhtar Trifi; Messaoud Mars; Néjib Hasnaoui

    2010-01-01

    The genetic diversity among Tunisian pomegranate cultivars has been investigated. Using universal primers, the random amplified polymorphic DNA (RAPD) method was used to generate banding profiles from a set of twelve cultivars. Data was then computed with appropriate programs to construct a dendrogram illustrating the relationships between the studied cultivars. Our data proved the efficiency of the designed method to examine the DNA polymorphism in this crop since the tested primers are char...

  13. The arils characterization of five different pomegranate (Punica granatum) genotypes stored after minimal processing technology.

    Ghasemnezhad, Mahmood; Zareh, Somayeh; Shiri, Mohammad Ali; Javdani, Zahra

    2015-04-01

    In this study, the quality of minimally processed different Iranian pomegranate genotypes was investigated during storage at 4 °C for 14 days. The results showed that at the end of storage time, the lowest microbial count was found on the arils of "Torsh Syabe Lorestan" genotype. There was a significant difference in titratable acidity, total soluble solids, total anthocyanin, catechin, and quercetin content in most genotypes after 14-day cold storage; while no difference was found between studied genotypes in antioxidant capacity, total phenolic, and flavonoid content. In general, total phenolic content and antioxidant capacity of pomegranate arils gradually decreased over storage time. Diphenolase activity of polyphenol oxidase (PPO) enzyme with dopamine hydrochloride substrate and peroxidase (POD) decreased over storage time, whereas diphenolase activity of PPO with pyrocatechol substrate significantly increased. The lowest diphenolase activity with dopamine hydrochloride and pyrocatechol substrates, as well as POD activity was found in Torsh Syabe Lorestan. The results suggested that the genotype of Torsh Syabe Lorestan, which showed the lowest microbial count on the arils and enzymes, could be more appropriate for minimal processing technology. PMID:25829582

  14. Punica granatum: A review on its potential role in treating periodontal disease

    Prasad, Divyashree; Kunnaiah, Ravi

    2014-01-01

    A wide variety of plants possess enormous treasure of medicinal value. Majority of these medicinal plants have been used to treat various systemic conditions successfully. Over the recent years, use of these medicinal plants has resurfaced to treat oral conditions. Among the oral conditions, periodontal disease remains one of the most common. Alternative and preventive options has become the need of the hour in order to overcome the adverse effects of the antimicrobial agents used in large as...

  15. Evaluation of antioxidant and antiradical properties of Pomegranate (Punica granatum L.) seed and defatted seed extracts.

    Basiri, Shadi

    2015-02-01

    Pomegranate seeds are byproducts of the Pomegranate juice industries that contains functional compounds such as phenols. This study was aimed to evaluate the effect of solvents on extraction from Pomegranate seed and Pomegranate defatted seed and to measure the yield extract and phenolic content and antioxidant properties. For this purpose, the seeds and defatted seeds were directly isolated from fruits and seeds by cold pressing respectively, then were crushed and extracted with different solvents, including water, Methanol, Acetone, Ethyl acetate and Hexane and finally the extracts of them were evaluted. Phenolic compounds, ferric reducing-antioxidant power and radicals scavenging property of extracts were measured. The results showed the highest extraction efficiencies were for Hexane and Acetone solvents in extraction of seed and defatted seed respectively. The highest phenolic content was obtained from Methanol seed extract. Reducing activity test proved that the Methanol extracts of Pomegranate seed and Pomegranate defatted seed had the highest reducing strength. Results of radical scavenging activity were similar to reducing activity results. The order of antioxidant capacity of Pomegranate seed and Pomegranate defatted seed were found to be Methanol > Water > Acetone > Butanol > Ethyl acetate > Hexane. It can be concluded Pomegranate seed, which possesses high levels of polyphenols, can be one of the sources of the natural antioxidants. The Methanol extract had a higher antioxidant efficiency than seed and defatted seed extracts. PMID:25694727

  16. Pomegranate juice (punica granatum: a new storage medium for avulsed teeth.

    Sara Tavassoli-Hojjati

    2014-04-01

    Full Text Available There is evidence indicating that pomegranate juice contains many of the essential properties necessary to retain cell viability and cell proliferation. These properties indicate that pomegranate juice is a suitable storage medium for avulsed teeth. However, this idea has not yet been tested. In this study, the capacity of pomegranate juice (PJ as a storage medium for retaining avulsed teeth was evaluated.PDL fibroblasts were obtained from healthy human premolars and cultured in Dulbecco's Modified Eagle's Medium (DMEM. Cultured cells were subjected to different concentrations of pomegranate juice (PJ, 1% Hank's balanced salt solution (HBSS and tap water for 1, 3, 6 and 24 hours. PDL cell viability was assessed by the neutral red uptake assay.The results indicated that 7.5% PJ was the most effective solution for maintaining PDL cell viability amongst all the experimental solution's and time intervals (P<0.05. The results also showed that 1% PJ was as effective as HBSS for maintaining PDL cell viability. The amount of cell viability increased with increasing concentration of PJ at all time intervals (P<0.001. This effect is suggestive of the proliferative potential of PJ solution.In conclusion, PJ can be recommended as a suitable transport medium for avulsed teeth.

  17. Bioactive properties of commercialised pomegranate (Punica granatum) juice: antioxidant, antiproliferative and enzyme inhibiting activities

    Les, F.; Prieto, J.M.; Arbonés-Mainar, J. M.; Valero, M. S.; López, V.

    2015-01-01

    Pomegranate juice and related products have long been used either in traditional medicine or as nutritional supplements claiming beneficial effects. Although there are several studies on this food plant, only a few studies have been performed with pomegranate juice or marketed products. The aim of this work is to evaluate the antioxidant effects of pomegranate juice on cellular models using hydrogen peroxide as an oxidizing agent or DPPH and superoxide radicals in cell free systems. The antip...

  18. Punica granatum (Pomegranate juice provides an HIV-1 entry inhibitor and candidate topical microbicide

    Li Yun-Yao

    2004-10-01

    Full Text Available Abstract Background For ≈ 24 years the AIDS pandemic has claimed ≈ 30 million lives, causing ≈ 14,000 new HIV-1 infections daily worldwide in 2003. About 80% of infections occur by heterosexual transmission. In the absence of vaccines, topical microbicides, expected to block virus transmission, offer hope for controlling the pandemic. Antiretroviral chemotherapeutics have decreased AIDS mortality in industrialized countries, but only minimally in developing countries. To prevent an analogous dichotomy, microbicides should be: acceptable; accessible; affordable; and accelerative in transition from development to marketing. Already marketed pharmaceutical excipients or foods, with established safety records and adequate anti-HIV-1 activity, may provide this option. Methods Fruit juices were screened for inhibitory activity against HIV-1 IIIB using CD4 and CXCR4 as cell receptors. The best juice was tested for inhibition of: (1 infection by HIV-1 BaL, utilizing CCR5 as the cellular coreceptor; and (2 binding of gp120 IIIB and gp120 BaL, respectively, to CXCR4 and CCR5. To remove most colored juice components, the adsorption of the effective ingredient(s to dispersible excipients and other foods was investigated. A selected complex was assayed for inhibition of infection by primary HIV-1 isolates. Results HIV-1 entry inhibitors from pomegranate juice adsorb onto corn starch. The resulting complex blocks virus binding to CD4 and CXCR4/CCR5 and inhibits infection by primary virus clades A to G and group O. Conclusion These results suggest the possibility of producing an anti-HIV-1 microbicide from inexpensive, widely available sources, whose safety has been established throughout centuries, provided that its quality is adequately standardized and monitored.

  19. Antimicrobial Analysis of an Antiseptic Made from Ethanol Crude Extracts of P. granatum and E. uniflora in Wistar Rats against Staphylococcus aureus and Staphylococcus epidermidis

    Thaís Honório Lins Bernardo

    2015-01-01

    Full Text Available Introduction. Surgical site infection remains a challenge for hospital infection control, especially when it relates to skin antisepsis in the surgical site. Objective. To analyze the antimicrobial activity in vivo of an antiseptic from ethanol crude extracts of P. granatum and E. uniflora against Gram-positive and Gram-negative bacteria. Methods. Agar drilling and minimal inhibitory tests were conducted for in vitro evaluation. In the in vivo bioassay were used Wistar rats and Staphylococcus aureus (ATCC 25923 and Staphylococcus epidermidis (ATCC 14990. Statistical analysis was performed through variance analysis and Scott-Knott cluster test at 5% probability and significance level. Results. In the in vitro, ethanolic extracts of Punica granatum and Eugenia uniflora and their combination showed the best antimicrobial potential against S. epidermidis and S. aureus. In the in vivo bioassay against S. epidermidis, there was no statistically significant difference between the tested product and the patterns used after five minutes of applying the product. Conclusion. The results indicate that the originated product is an antiseptic alternative source against S. epidermidis compared to chlorhexidine gluconate. It is suggested that further researches are to be conducted in different concentrations of the test product, evaluating its effectiveness and operational costs.

  20. Optimization of Callua Induction Conditions of Vitro Leaves from Soft-seed Punica granatm L.%软籽石榴离体叶片愈伤组织诱导条件的优化

    彭丽萍; 郑雷

    2011-01-01

    [目的]研究软籽石榴离体叶片愈伤组织诱导的最佳条件.[方法]以软籽石榴的幼嫩叶片为外植体,以MS为基本培养基,研究了不同浓度和类型的植物生长调节剂、叶片的不同部分、不同糖浓度和类型对愈伤组织诱导的影响.[结果]培养基MS+0.1 mg/LNAA+0.1 mg/L IBA+1.0 mg/L6-BA+30 g/L蔗糖对愈伤组织的诱导效果最好,选择叶片中部或叶片基部为外植体,可以有效提高诱导率,降低褐化率,诱导率最高达93.3%,褐化率最低达23.3%.[结论]为石榴的离体快繁、种质保存和利用基因工程技术改良品质奠定一定的理论和实践基础.%[ Objective] The aim was to study the best conditions of callus induction conditions of vitro leaves from soft-secd Punica granatum L. [ Method ] The vitro leaves of soft-seed Punica granatum L. was as explant, MS was basic medium. Effect of different concentrations and types of hormone , different parts of the leaves and different sugar concentration and types on callus induction were studyed. [ Result ] The results showed that the medium MS + 0. 1 mg/L/NAA + 0. 1 mg/L IBA + 1.0 mg/L 6-BA + 30 g/L sucrose was best for callus induction, the base or midcdle of leaves(including part of petiole) was as explant 1,lt could improve callus rate and reduce browning rate. [ Conclusion]The study provided theory and practice basic for callus induction, germinal conversation and improving quality. of soft-seed Punica granatum L

  1. Resources of Punica granatum Linn. and Its Exploitation and Utilization in Xinjiang%新疆石榴(Punica L.)资源及其开发利用

    刘文江

    2007-01-01

    石榴作为新疆一种绿色食品资源,受到国内外市场广泛关注,使得新疆近几年石榴的种植面积迅速扩大.本文对新疆石榴生物学特性、种类、品种和开发利用价值等方面进行了详细论述,阐明了新疆大力发展石榴产业的优势,并在分析现状和存在问题的基础上,提出了新疆发展石榴产业的几点建议.

  2. Genetic diversity of pomegranate (Punica granatum L.) population in China by RAMP markers%中国石榴(Punica granatum L.)居群遗传多样性

    赵丽华

    2013-01-01

    为了更有效地保护和利用石榴种质资源,利用RAMP标记对中国7个石榴主产区的46个石榴品种进行遗传分析.结果显示:14对RAMP引物共扩出127条DNA条带,其中113条显示多态性,多态位点百分率(P)为88.98%;7个石榴居群遗传多样性依次为:山东居群>陕西居群>云南居群>河南居群>安徽居群>四川居群>新疆居群;7个居群间的基因分化系数(Gst)为0.183 0;群间基因流的估测值(Nm)为2.231 5.表明中国石榴具有较大的遗传变异及丰富的遗传多样性,居群间存在较强的基因流.%To protect and utilize pomegranate resources in China,random amplified microsatellite polymorphism (RAMP) technique was used to analyze the genetic relationships among 46 pomegranate genotypes collected from seven major production areas in China.A total of 127 bands were amplified by fourteen pairs of high polymorphism primers,among which 113 (88.98%) were polymorphic loci.The genetic diversity of seven pomegranate populations followed the order of Shandong > Shannxi > Yunnan > Henan > Anhui > Sichuan > Xinjiang.The gene differentiation coefficient (Gst) of seven populations was 0.183 0,and the gene flow (Nm) was 2.231 5.These statistics indicated that the present pomegranate resources in China had high genetic variation and genetic diversity,and a strong gene flow existed among these populations.

  3. Antioxidant and Anti-Inflammatory Activities of Pomegranate (Punica granatum on Eimeria papillata-Induced Infection in Mice

    Omar S. O. Amer

    2015-01-01

    Full Text Available Coccidiosis is the most prevalent disease causing widespread economic loss, especially in poultry farms. Here, we investigated the effects of pomegranate peel extract (PPE on the outcome of coccidiosis caused by Eimeria papillata in mice. The data showed that mice infected with E. papillata and treated with PPE revealed a significant decrease in the output of oocysts in their faeces by day 5 p.i. Infection also induced inflammation and injury of the jejunum. This was evidenced (i as increases in reactive oxygen species, (ii, as increased neutrophils and decreased lymphocytes in blood (ii as increased mRNA levels of inducible nitric oxide synthase (iNOS, Bcl-2 gene, and of the cytokines interferon gamma (IFN-γ, tumour necrosis factor-α (TNF-α, and interleukin-1β (IL-1β, and (iv as downregulation of mucin gene MUC2 mRNA. All these infection-induced parameters were significantly altered during PPE treatment. In particular, PPE counteracted the E. papillata-induced loss of the total antioxidant capacity. Our data indicated that PPE treatment significantly attenuated inflammation and injury of the jejunum induced by E. papillata infections.

  4. Formulasi dan Uji Efek Anti-Aging dari Krim Mengandung Ekstrak Kulit Buah Delima (Punica granatum L)

    Ramadhilla, Nailul

    2015-01-01

    Anti-aging is a process that useful to prevent or slow down the effects of aging. Pomegranate peel is rich with flavonoids, phenolic acids, catechins and tannins which are using as an antioxidant. The objective of this study is to prepare an anti-aging cream using pomegranate peel extract and determine how long its to provide an anti-aging effects. This study was carried out experimentally. Pomegranate peel extracted by maceration using ethanol 70% and concentrated by using rotary evaporat...

  5. Pomegranate (Punica granatum Peel Extract as a Green Corrosion Inhibitor for Mild Steel in Hydrochloric Acid Solution

    Habib Ashassi-Sorkhabi

    2015-01-01

    Full Text Available The inhibition effect of pomegranate peel extract (PPE on the corrosion of mild steel in hydrochloric acid (HCl solution was investigated. The polarization, mass loss, and electrochemical impendence techniques were used to evaluate the corrosion inhibition performance of the pomegranate peel extract. The results revealed that PPE acts as a corrosion inhibitor in HCl solution. The inhibition efficiency increased with the increase of extract concentration. The inhibition action was attributed to the adsorption of the chemical compounds present in the extract solution, on mild steel surface.

  6. Influence of putrescine and carnauba wax on functional and sensory quality of pomegranate (Punica granatum L.) fruits during storage

    Barman, Kalyan; Asrey, Ram; Pal, R. K.; Kaur, Charanjit; Jha, S. K.

    2011-01-01

    Functional properties (anthocyanins, antioxidant, ascorbic acid and tannin) and sensory score were determined in pomegranate fruits at two storage temperatures (3 and 5 °C) after treatment with 2 mM putrescine and 1 : 10 carnauba wax (carnauba wax : water). The treatments (putrescine and carnauba wax) were given by immersion method followed by storage up to 60 days. Both treatments retained significantly higher anthocyanins, antioxidant, ascorbic acid, tannin and sensory qualities as compared...

  7. Study on the Mechanism of Browning of Pomegranate (Punica granatum L.cv. Ganesh) Peel in Different Storage Conditions

    2008-01-01

    Peel browning of pomegranate fruit is the key problem during storage. The mechanism of it during storage in relation to the technology of the browning control was studied in this paper. The effects of storage temperature, gas component and Ph value on peel browning of sweet pomegranate fruit in Lintong, Shaanxi Province, China, were investigated. Change in tannin content was measured and the relationship between the activities of ascorbic acid oxidase (AAO), polypenoloxidase (PPO), peroxidase (POD), catalase (CAT) and peel browning was analysed. The results showed tannin was the basic substance of pomegranate peel browning. The activities of the browning index of peel were correlated positively with AAO, PPO and POD, correlated negatively with CAT activity. Application of intermittent warming for 24 h at (15 ±0.5)℃ every 5 days for pomegranate fruit storage under the conditions of Ph 4.0, (5.0 ± 0.5)℃ and 5.0% CO2 + 8.0% O2 +87.0% N2 gas component could delay browning, with the browning index being 0.15 after 120 days of storage. Enzymatic tannin denaturation was the main cause that led to peel browning of pomegranate fruit during storage. The browning control of pomegranate fruit peel was effective using intermittent warming storage with suitable Ph, temperature and gas component.

  8. High-capacity calcium-binding chitinase III from pomegranate seeds (Punica granatum Linn.) is located in amyloplasts

    Lv, Chenyan; Masuda, Taro; Yang, Haixia; Sun, Lei; Zhao, Guanghua

    2011-01-01

    We have recently identified a new class III chitinase from pomegranate seeds (PSC). Interestingly, this new chitinase naturally binds calcium ions with high capacity and low affinity, suggesting that PSC is a Ca-storage protein. Analysis of the amino acid sequence showed that this enzyme is rich in acidic amino acid residues, especially Asp, which are responsible for calcium binding. Different from other known chitinases, PSC is located in the stroma of amyloplasts in pomegranate seeds. Trans...

  9. Irradiation Effect on the antioxidant properties, anti-microbial and cytoprotective of the bark of Punica granatum

    The bark of pomegranate has been used for some years to treat various health problems . Several studies have focused on specifying these problems, including antibacterial , antioxidant and cytoprotective . The use of pomegranate rind powder is an effective treatment against gastric ulcer and intestines and to strengthen the wall of the gastrointestinal tract. In this work, we studied the effects of gamma irradiation on the type antibacterial, anti-ulcer and bark grenade. This study was conducted on powdered pomegranate bark irradiated by applying decreasing radiation doses from 25kGy to 1.25KGy. All of our results shows that irradiation with a low degree improves the effectiveness of pomegranate bark for the treatment of gastric ulcer , however high degree irradiation enhances the antibacterial activity of bark pomegranate against Staphylococcus aureus.

  10. Preparation and antibacterial activities of Ag/Ag+/Ag3+ nanoparticle composites made by pomegranate (Punica granatum) rind extract

    Yang, Hui; Ren, Yan-yu; Wang, Tao; Wang, Chuang

    Nano-silver and its composite materials are widely used in medicine, food and other industries due to their strong conductivity, size effect and other special performances. So far, more microbial researches have been applied, but a plant method is rarely reported. In order to open up a new way to prepare AgNP composites, pomegranate peel extract was used in this work to reduce Ag+ to prepare Ag/Ag+/Ag3+ nanoparticle composites. UV-Vis was employed to detect and track the reduction of Ag+ and the forming process of AgNPs. The composition, structure and size of the crystal were analyzed by XRD and TEM. Results showed that, under mild conditions, pomegranate peel extract reacted with dilute AgNO3 solution to produce Ag/Ag+/Ag3+ nanoparticle composites. At pH = 8 and 10 mmol/L of AgNO3 concentration, the size of the achieved composites ranged between 15 and 35 nm with spherical shapes and good crystallinity. The bactericidal experiment indicated that the prepared Ag/Ag+/Ag3+ nanoparticles had strong antibacterial activity against gram positive bacteria and gram negative bacteria. FTIR analysis revealed that biological macromolecules with groups of sbnd NH2, sbnd OH, and others were distributed on the surface of the newly synthesized Ag/Ag+/Ag3+ nanoparticles. This provided a useful clue to further study the AgNP biosynthesis mechanism.