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Sample records for actinium 213

  1. Renal uptake of bismuth-213 and its contribution to kidney radiation dose following administration of actinium-225-labeled antibody

    Schwartz, J; O' Donoghue, J A; Humm, J L [Department of Medical Physics, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065 (United States); Jaggi, J S [Bristol-Myers Squibb, Plainsboro, NJ (United States); Ruan, S; Larson, S M [Nuclear Medicine Service Department of Radiology, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065 (United States); McDevitt, M; Scheinberg, D A, E-mail: schwarj1@mskcc.org [Molecular Pharmacology and Chemistry, Sloan-Kettering Institute, 1275 York Avenue, New York, NY 10065 (United States)

    2011-02-07

    Clinical therapeutic studies using {sup 225}Ac-labeled antibodies have begun. Of major concern is renal toxicity that may result from the three alpha-emitting progeny generated following the decay of {sup 225}Ac. The purpose of this study was to determine the amount of {sup 225}Ac and non-equilibrium progeny in the mouse kidney after the injection of {sup 225}Ac-huM195 antibody and examine the dosimetric consequences. Groups of mice were sacrificed at 24, 96 and 144 h after injection with {sup 225}Ac-huM195 antibody and kidneys excised. One kidney was used for gamma ray spectroscopic measurements by a high-purity germanium (HPGe) detector. The second kidney was used to generate frozen tissue sections which were examined by digital autoradiography (DAR). Two measurements were performed on each kidney specimen: (1) immediately post-resection and (2) after sufficient time for any non-equilibrium excess {sup 213}Bi to decay completely. Comparison of these measurements enabled estimation of the amount of excess {sup 213}Bi reaching the kidney ({gamma}-ray spectroscopy) and its sub-regional distribution (DAR). The average absorbed dose to whole kidney, determined by spectroscopy, was 0.77 (SD 0.21) Gy kBq{sup -1}, of which 0.46 (SD 0.16) Gy kBq{sup -1} (i.e. 60%) was due to non-equilibrium excess {sup 213}Bi. The relative contributions to renal cortex and medulla were determined by DAR. The estimated dose to the cortex from non-equilibrium excess {sup 213}Bi (0.31 (SD 0.11) Gy kBq{sup -1}) represented {approx}46% of the total. For the medulla the dose contribution from excess {sup 213}Bi (0.81 (SD 0.28) Gy kBq{sup -1}) was {approx}80% of the total. Based on these estimates, for human patients we project a kidney-absorbed dose of 0.28 Gy MBq{sup -1} following administration of {sup 225}Ac-huM195 with non-equilibrium excess {sup 213}Bi responsible for approximately 60% of the total. Methods to reduce renal accumulation of radioactive progeny appear to be necessary for the

  2. Production of Actinium-225 via High Energy Proton Induced Spallation of Thorium-232

    The science of cancer research is currently expanding its use of alpha particle emitting radioisotopes. Coupled with the discovery and proliferation of molecular species that seek out and attach to tumors, new therapy and diagnostics are being developed to enhance the treatment of cancer and other diseases. This latest technology is commonly referred to as Alpha Immunotherapy (AIT). Actinium-225/Bismuth-213 is a parent/daughter alpha-emitting radioisotope pair that is highly sought after because of the potential for treating numerous diseases and its ability to be chemically compatible with many known and widely used carrier molecules (such as monoclonal antibodies and proteins/peptides). Unfortunately, the worldwide supply of actinium-225 is limited to about 1,000mCi annually and most of that is currently spoken for, thus limiting the ability of this radioisotope pair to enter into research and subsequently clinical trials. The route proposed herein utilizes high energy protons to produce actinium-225 via spallation of a thorium-232 target. As part of previous R and D efforts carried out at Argonne National Laboratory recently in support of the proposed US FRIB facility, it was shown that a very effective production mechanism for actinium-225 is spallation of thorium-232 by high energy proton beams. The base-line simulation for the production rate of actinium-225 by this reaction mechanism is 8E12 atoms per second at 200 MeV proton beam energy with 50 g/cm2 thorium target and 100 kW beam power. An irradiation of one actinium-225 half-life (10 days) produces ∼100 Ci of actinium-225. For a given beam current the reaction cross section increases slightly with energy to about 400 MeV and then decreases slightly for beam energies in the several GeV regime. The object of this effort is to refine the simulations at proton beam energies of 400 MeV and above up to about 8 GeV. Once completed, the simulations will be experimentally verified using 400 MeV and 8 GeV protons

  3. Production of Actinium-225 via High Energy Proton Induced Spallation of Thorium-232

    Harvey, James T.; Nolen, Jerry; Vandergrift, George; Gomes, Itacil; Kroc, Tom; Horwitz, Phil; McAlister, Dan; Bowers, Del; Sullivan, Vivian; Greene, John

    2011-12-30

    The science of cancer research is currently expanding its use of alpha particle emitting radioisotopes. Coupled with the discovery and proliferation of molecular species that seek out and attach to tumors, new therapy and diagnostics are being developed to enhance the treatment of cancer and other diseases. This latest technology is commonly referred to as Alpha Immunotherapy (AIT). Actinium-225/Bismuth-213 is a parent/daughter alpha-emitting radioisotope pair that is highly sought after because of the potential for treating numerous diseases and its ability to be chemically compatible with many known and widely used carrier molecules (such as monoclonal antibodies and proteins/peptides). Unfortunately, the worldwide supply of actinium-225 is limited to about 1,000mCi annually and most of that is currently spoken for, thus limiting the ability of this radioisotope pair to enter into research and subsequently clinical trials. The route proposed herein utilizes high energy protons to produce actinium-225 via spallation of a thorium-232 target. As part of previous R and D efforts carried out at Argonne National Laboratory recently in support of the proposed US FRIB facility, it was shown that a very effective production mechanism for actinium-225 is spallation of thorium-232 by high energy proton beams. The base-line simulation for the production rate of actinium-225 by this reaction mechanism is 8E12 atoms per second at 200 MeV proton beam energy with 50 g/cm2 thorium target and 100 kW beam power. An irradiation of one actinium-225 half-life (10 days) produces {approx}100 Ci of actinium-225. For a given beam current the reaction cross section increases slightly with energy to about 400 MeV and then decreases slightly for beam energies in the several GeV regime. The object of this effort is to refine the simulations at proton beam energies of 400 MeV and above up to about 8 GeV. Once completed, the simulations will be experimentally verified using 400 MeV and 8 Ge

  4. Extraction of actinium with di-(2-ethylhexyl)phosphoric acid from hydrochloric and nitric acid solutions

    The extraction of actinium with HDEHP from Cl- and NO3- systems has been investigated. It was found that extraction of actinium from HCl solutions is much better than from HNO3 solutions. Stability constants of actinium complexes Ac(X-)+2 with Cl- and NO3- ligands were determined. Our results show that the actinium formed less stable complexes with Cl- than with NO3- ligands. 5 refs., 3 figs., 1 tab. (author)

  5. The sorption of polonium, actinium and protactinium onto geological materials

    This paper describes a combined experimental and modeling program of generic sorption studies to increase confidence in the performance assessment for a potential high-level radioactive waste repository in Japan. The sorption of polonium, actinium and protactinium onto geological materials has been investigated. Sorption of these radioelements onto bentonite, tuff and granodiorite from equilibrated de-ionized water was studied under reducing conditions at room temperature. In addition, the sorption of actinium and protactinium was investigated at 60 C. Thermodynamic chemical modeling was carried out to aid interpretation of the results

  6. Separation of Actinium 227 from the uranium minerals

    The purpose of this work was to separate Actinium 227, whose content is 18%, from the mineral carnotite found in Gomez Chihuahua mountain range in Mexico. The mineral before processing is is pre-concentrated and passed, first through anionic exchange resins, later the eluate obtained is passed through cationic resins. The resins were 20-50 MESH QOWEX and 100-200 MESH 50 X 8-20 in some cased 200-400 MESH AG 50W-X8, 1X8 in other cases. The eluates from the ionic exchange were electrodeposited on stainless steel polished disc cathode and platinum electrode as anode; under a current ODF 10mA for 2.5 to 5 hours and of 100mA for .5 of an hour. it was possible to identify the Actinium 227 by means of its descendents, TH-227 and RA-223, through alpha spectroscopy. Due to the radiochemical purity which the electro deposits were obtained the Actinium 227 was low and was not quantitatively determined. A large majority of the members of the natural radioactive series 3 were identified and even alpha energies reported in the literature with very low percentages of non-identified emissions were observed. We conclude that a more precise study is needed concerning ionic exchange and electrodeposit to obtain an Actinium 227 of radiochemical purity. (Author)

  7. Spectroscopic and computational investigation of actinium coordination chemistry.

    Ferrier, Maryline G; Batista, Enrique R; Berg, John M; Birnbaum, Eva R; Cross, Justin N; Engle, Jonathan W; La Pierre, Henry S; Kozimor, Stosh A; Lezama Pacheco, Juan S; Stein, Benjamin W; Stieber, S Chantal E; Wilson, Justin J

    2016-01-01

    Actinium-225 is a promising isotope for targeted-α therapy. Unfortunately, progress in developing chelators for medicinal applications has been hindered by a limited understanding of actinium chemistry. This knowledge gap is primarily associated with handling actinium, as it is highly radioactive and in short supply. Hence, Ac(III) reactivity is often inferred from the lanthanides and minor actinides (that is, Am, Cm), with limited success. Here we overcome these challenges and characterize actinium in HCl solutions using X-ray absorption spectroscopy and molecular dynamics density functional theory. The Ac-Cl and Ac-OH2O distances are measured to be 2.95(3) and 2.59(3) Å, respectively. The X-ray absorption spectroscopy comparisons between Ac(III) and Am(III) in HCl solutions indicate Ac(III) coordinates more inner-sphere Cl(1-) ligands (3.2±1.1) than Am(III) (0.8±0.3). These results imply diverse reactivity for the +3 actinides and highlight the unexpected and unique Ac(III) chemical behaviour. PMID:27531582

  8. Discovery of the actinium, thorium, protactinium, and uranium isotopes

    Fry, C; Thoennessen, M

    2012-01-01

    Currently, 31 actinium, 31 thorium, 28 protactinium, and 23 uranium isotopes have so far been observed; the discovery of these isotopes is discussed. For each isotope a brief summary of the first refereed publication, including the production and identification method, is presented.

  9. Production of high-purity radium-223 from legacy actinium-beryllium neutron sources.

    Soderquist, Chuck Z; McNamara, Bruce K; Fisher, Darrell R

    2012-07-01

    Radium-223 is a short-lived alpha-particle-emitting radionuclide with potential applications in cancer treatment. Research to develop new radiopharmaceuticals employing (223)Ra has been hindered by poor availability due to the small quantities of parent actinium-227 available world-wide. The purpose of this study was to develop innovative and cost-effective methods to obtain high-purity (223)Ra from (227)Ac. We obtained (227)Ac from two surplus actinium-beryllium neutron generators. We retrieved the actinium/beryllium buttons from the sources and dissolved them in a sulfuric-nitric acid solution. A crude actinium solid was recovered from the solution by coprecipitation with thorium fluoride, leaving beryllium in solution. The crude actinium was purified to provide about 40 milligrams of actinium nitrate using anion exchange in methanol-water-nitric acid solution. The purified actinium was then used to generate high-purity (223)Ra. We extracted (223)Ra using anion exchange in a methanol-water-nitric acid solution. After the radium was separated, actinium and thorium were then eluted from the column and dried for interim storage. This single-pass separation produces high purity, carrier-free (223)Ra product, and does not disturb the (227)Ac/(227)Th equilibrium. A high purity, carrier-free (227)Th was also obtained from the actinium using a similar anion exchange in nitric acid. These methods enable efficient production of (223)Ra for research and new alpha-emitter radiopharmaceutical development. PMID:22697483

  10. Radium, thorium, and actinium extraction from seawater using an improved manganese-oxide-coated fiber

    Laboratory experiments were conducted to determine the efficiency with which improved manganese-oxide-coated acrylic fibers extract radium, thorium, and actinium from seawater. Tests were made using surface seawater spiked with 227Ac, 227Th and 223Ra. For sample volumes of approximately 30 liters and flow rates up to 0.5 liters per minute, radium and actinium are removed quantitatively. Approximately 80-95% of the thorium is removed under these same conditions. (Auth.)

  11. Neutron-Induced Fission of Actinium-227, Protactinium-231 and Neptunium-237: Mass Distribution

    Results of radiochemical studies on the mass distribution in the neutron-induced fission of actinium-227, protactinium-231 and neptunium-237 have been presented. This work has been carried out as part of a programme to determine the mass distribution in the fission of heavy elements as a function of Z and A. All irradiations have been carried out in the core of the swimming-pool type reactor APSARA with cadmium shielding wherever necessary. Relative yields of several fission product nuclides have been obtained by a method involving a comparison of the fission product activities from the respective targets with those formed from uranium-235 simultaneously irradiated. Thermal-neutron fission yields of uranium-235 have been assumed. These results indicate a predominantly asymmetric mass distribution in all the three cases, and also a distinct though small symmetric peak in the case of actinium-227. (author)

  12. A new method for the determination of low-level actinium-227 in geological samples

    We developed a new method for the determination of 227Ac in geological samples. The method uses extraction chromatographic techniques and alpha-spectrometry and is applicable for a range of natural matrices. Here we report on the procedure and results of the analysis of water (fresh and seawater) and rock samples. Water samples were acidified and rock samples underwent total dissolution via acid leaching. A DGA (N,N,N',N'-tetra-n-octyldiglycolamide) extraction chromatographic column was used for the separation of actinium. The actinium fraction was prepared for alpha spectrometric measurement via cerium fluoride micro-precipitation. Recoveries of actinium in water samples were 80 ± 8 % (number of analyses n = 14) and in rock samples 70 ± 12 % (n = 30). The minimum detectable activities (MDA) were 0.017-0.5 Bq kg-1 for both matrices. Rock sample 227Ac activities ranged from 0.17 to 8.3 Bq kg-1 and water sample activities ranged from below MDA values to 14 Bq kg-1of 227Ac. From the analysis of several standard rock and water samples with the method we found very good agreement between our results and certified values. (author)

  13. Analysis of the gamma spectra of the uranium, actinium, and thorium decay series

    Momeni, M.H.

    1981-09-01

    This report describes the identification of radionuclides in the uranium, actinium, and thorium series by analysis of gamma spectra in the energy range of 40 to 1400 keV. Energies and absolute efficiencies for each gamma line were measured by means of a high-resolution germanium detector and compared with those in the literature. A gamma spectroscopy method, which utilizes an on-line computer for deconvolution of spectra, search and identification of each line, and estimation of activity for each radionuclide, was used to analyze soil and uranium tailings, and ore.

  14. Analysis of the gamma spectra of the uranium, actinium, and thorium decay series

    This report describes the identification of radionuclides in the uranium, actinium, and thorium series by analysis of gamma spectra in the energy range of 40 to 1400 keV. Energies and absolute efficiencies for each gamma line were measured by means of a high-resolution germanium detector and compared with those in the literature. A gamma spectroscopy method, which utilizes an on-line computer for deconvolution of spectra, search and identification of each line, and estimation of activity for each radionuclide, was used to analyze soil and uranium tailings, and ore

  15. In-source laser spectroscopy developments at TRILIS—towards spectroscopy on actinium and scandium

    Resonance Ionization Laser Ion Sources (RILIS) have become a versatile tool for production and study of exotic nuclides at Isotope Separator On-Line (ISOL) facilities such as ISAC at TRIUMF. The recent development and addition of a grating tuned spectroscopy laser to the TRIUMF RILIS solid state laser system allows for wide range spectral scans to investigate atomic structures on short lived isotopes, e.g., those from the element actinium, produced in uranium targets at ISAC. In addition, development of new and improved laser ionization schemes for rare isotope production at ISAC is ongoing. Here spectroscopic studies on bound states, Rydberg states and autoionizing (AI) resonances on scandium using the existing off-line capabilities are reported. These results allowed to identify a suitable ionization scheme for scandium via excitation into an autoionizing state at 58,104 cm − 1 which has subsequently been used for ionization of on-line produced exotic scandium isotopes.

  16. 32 CFR 213.4 - Policy.

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Policy. 213.4 Section 213.4 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS SUPPORT FOR NON-FEDERAL ENTITIES AUTHORIZED TO OPERATE ON DOD INSTALLATIONS § 213.4 Policy. It is DoD policy...

  17. 29 CFR 780.213 - Produce business.

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Produce business. 780.213 Section 780.213 Labor Regulations... Specific Situations Hatchery Operations § 780.213 Produce business. In some instances, hatcheries also engage in the produce business as such and commingle with the culled eggs and chickens other eggs...

  18. 42 CFR 93.213 - Institution.

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Institution. 93.213 Section 93.213 Public Health... MISCONDUCT Definitions § 93.213 Institution. Institution means any individual or person that applies for or... or other research institutes, small research institutions, and independent researchers....

  19. 7 CFR 1400.213 - Military personnel.

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Military personnel. 1400.213 Section 1400.213 Agriculture Regulations of the Department of Agriculture (Continued) COMMODITY CREDIT CORPORATION, DEPARTMENT... AND SUBSEQUENT CROP, PROGRAM, OR FISCAL YEARS Payment Eligibility § 1400.213 Military personnel. If...

  20. 49 CFR 1542.213 - Training.

    2010-10-01

    ... 49 Transportation 9 2010-10-01 2010-10-01 false Training. 1542.213 Section 1542.213 Transportation..., DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY AIRPORT SECURITY Operations § 1542.213 Training. (a... training in accordance with TSA-approved curriculum specified in the security program. This curriculum...

  1. 22 CFR 213.5 - Fraud claims.

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Fraud claims. 213.5 Section 213.5 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT CLAIMS COLLECTION General § 213.5 Fraud claims. (a) The CFO will refer claims involving fraud, the presentation of a false claim, or misrepresentation on the...

  2. 32 CFR 213.5 - Responsibilities.

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Responsibilities. 213.5 Section 213.5 National... SUPPORT FOR NON-FEDERAL ENTITIES AUTHORIZED TO OPERATE ON DOD INSTALLATIONS § 213.5 Responsibilities. (a... of non-Federal entities on DoD installations. (3) Assign responsibilities to the DoD Components...

  3. 28 CFR 21.3 - Aliens.

    2010-07-01

    ... 28 Judicial Administration 1 2010-07-01 2010-07-01 false Aliens. 21.3 Section 21.3 Judicial Administration DEPARTMENT OF JUSTICE WITNESS FEES § 21.3 Aliens. (a) Aliens entitled to payment of $30 per day. The following aliens are entitled to witness fees and allowances provided in § 21.4: (1)...

  4. 42 CFR 59.213 - [Reserved

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false 59.213 Section 59.213 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GRANTS GRANTS FOR FAMILY PLANNING SERVICES Grants for Family Planning Service Training § 59.213...

  5. 49 CFR 213.123 - Tie plates.

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Tie plates. 213.123 Section 213.123 Transportation... TRANSPORTATION TRACK SAFETY STANDARDS Track Structure § 213.123 Tie plates. (a) In Classes 3 through 5 track where timber crossties are in use there shall be tie plates under the running rails on at least eight...

  6. 12 CFR 213.7 - Advertising.

    2010-01-01

    ... 12 Banks and Banking 2 2010-01-01 2010-01-01 false Advertising. 213.7 Section 213.7 Banks and Banking FEDERAL RESERVE SYSTEM BOARD OF GOVERNORS OF THE FEDERAL RESERVE SYSTEM CONSUMER LEASING (REGULATION M) § 213.7 Advertising. (a) General rule. An advertisement for a consumer lease may state that...

  7. Developments towards in-gas-jet laser spectroscopy studies of actinium isotopes at LISOL

    Raeder, S.; Bastin, B.; Block, M.; Creemers, P.; Delahaye, P.; Ferrer, R.; Fléchard, X.; Franchoo, S.; Ghys, L.; Gaffney, L. P.; Granados, C.; Heinke, R.; Hijazi, L.; Huyse, M.; Kron, T.; Kudryavtsev, Yu.; Laatiaoui, M.; Lecesne, N.; Luton, F.; Moore, I. D.; Martinez, Y.; Mogilevskiy, E.; Naubereit, P.; Piot, J.; Rothe, S.; Savajols, H.; Sels, S.; Sonnenschein, V.; Traykov, E.; Van Beveren, C.; Van den Bergh, P.; Van Duppen, P.; Wendt, K.; Zadvornaya, A.

    2016-06-01

    To study exotic nuclides at the borders of stability with laser ionization and spectroscopy techniques, highest efficiencies in combination with a high spectral resolution are required. These usually opposing requirements are reconciled by applying the in-gas-laser ionization and spectroscopy (IGLIS) technique in the supersonic gas jet produced by a de Laval nozzle installed at the exit of the stopping gas cell. Carrying out laser ionization in the low-temperature and low density supersonic gas jet eliminates pressure broadening, which will significantly improve the spectral resolution. This article presents the required modifications at the Leuven Isotope Separator On-Line (LISOL) facility that are needed for the first on-line studies of in-gas-jet laser spectroscopy. Different geometries for the gas outlet and extraction ion guides have been tested for their performance regarding the acceptance of laser ionized species as well as for their differential pumping capacities. The specifications and performance of the temporarily installed high repetition rate laser system, including a narrow bandwidth injection-locked Ti:sapphire laser, are discussed and first preliminary results on neutron-deficient actinium isotopes are presented indicating the high capability of this novel technique.

  8. 14 CFR 21.213 - Eligibility.

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Eligibility. 21.213 Section 21.213 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT CERTIFICATION... manufacturer who is a United States citizen may apply for a Class I or Class II provisional...

  9. 31 CFR 31.213 - General standards.

    2010-07-01

    ... to the Treasury that is relating to services under EESA or required pursuant to 31 CFR Part 31 is... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false General standards. 31.213 Section 31... Conflicts of Interest § 31.213 General standards. (a) During the time period in which a retained entity...

  10. Nuclear Data Sheets for A=213

    Evaluated spectroscopic data and level schemes from radioactive decay and nuclear reaction studies are presented for all nuclei with mass number A=213. This evaluation for A=213 supersedes the earlier one by Y. A. Akovali (1992Ak01), published in Nuclear Data Sheets 66, 237 (1992)

  11. 31 CFR 800.213 - Foreign government.

    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Foreign government. 800.213 Section... TAKEOVERS BY FOREIGN PERSONS Definitions § 800.213 Foreign government. The term foreign government means any government or body exercising governmental functions, other than the United States Government or...

  12. 21 CFR 21.3 - Definitions.

    2010-04-01

    ... committee members, State and local officials, and consumers are considered individuals. (b) Records about... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Definitions. 21.3 Section 21.3 Food and Drugs FOOD... products regulated by the Food and Drug Administration or with which the Food and Drug Administration...

  13. 45 CFR 213.25 - Evidence.

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false Evidence. 213.25 Section 213.25 Public Welfare... Evidence. (a) Testimony. Testimony shall be given orally under oath or affirmation by witnesses at the... hearing if the presiding officer so requires. (c) Rules of evidence. Technical rules of evidence shall...

  14. Dicty_cDB: SSA213 [Dicty_cDB

    Full Text Available SS (Link to library) SSA213 (Link to dictyBase) - - - Contig-U16397-1 SSA213Z (Link... to Original site) - - SSA213Z 537 - - - - Show SSA213 Library SS (Link to library) Clone ID SSA213 (Link to...ycdb.biol.tsukuba.ac.jp/CSM/SS/SSA2-A/SSA213Q.Seq.d/ Representative seq. ID SSA21...3Z (Link to Original site) Representative DNA sequence >SSA213 (SSA213Q) /CSM/SS/SSA2-A/SSA213Q.Seq.d/ XXXXX...lkk*k*k**flivk k Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value SSA

  15. New method for large scale production of medically applicable Actinium-225 and Radium-223

    Alpha-emitters (211At, 212Bi, 213Bi, 223Ra, 225Ac) are promising for targeted radiotherapy of cancer. Only two alpha decays near a cell membrane result in 50% death of cancer cell and only a single decay inside the cell is required for this. 225Ac may be used either directly or as a mother radionuclide in 213Bi isotope generator. Production of 225Ac is provided by three main suppliers - Institute for Transuranium Elements in Germany, Oak Ridge National Laboratory in USA and Institute of Physics and Power Engineering in Obninsk, Russia. The current worldwide production of 225Ac is approximately 1.7 Ci per year that corresponds to only 100-200 patients that could be treated annually. The common approach for 225Ac production is separation from mother 229Th or irradiation of 226Ra with protons in a cyclotron. Both the methods have some practical limitations to be applied routinely. 225Ac can be also produced by irradiation of natural thorium with medium energy protons . Cumulative cross sections of 225Ac, 227Ac, 227Th, 228Th formations have been obtained recently. Thorium targets (1-9 g) were irradiated by 114-91 MeV proton beam (1-50 μA) at INR linear accelerator. After dissolution in 8 M HNO3 + 0.004 M HF thorium was removed by double LLX by HDEHP in toluene (1:1). Ac and REE were pre-concentrated and separated from Ra and most fission products by DGA-Resin (Triskem). After washing out by 0.01 M HNO3 Ac was separated from REE by TRU Resin (Triskem) in 3 M HNO3 media. About 6 mCi 225Ac were separated in hot cell with chemical yield 85%. The method may be upscaled for production of Ci amounts of the radionuclide. The main impurity is 227Ac (0.1% at the EOB) but it does not hinder 225Ac from being used for medical 225Ac/213Bi generators. (author)

  16. 47 CFR 213.6 - Criteria.

    2010-10-01

    ... CORRESPONDENCE TELECOMMUNICATIONS PRECEDENCE SYSTEM § 213.6 Criteria. (a) Flash, Flash Emergency. (1) This is the...) Flash, or Flash Emergency telephone calls or messages shall be handled in the order received and ahead... necessary to obtain a circuit for a Flash, or Flash Emergency call any call in progress of a...

  17. 27 CFR 9.213 - Snipes Mountain.

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Snipes Mountain. 9.213... Snipes Mountain. (a) Name. The name of the viticultural area described in this section is “Snipes Mountain”. For purposes of part 4 of this chapter, “Snipes Mountain” is a term of viticultural...

  18. 25 CFR 213.15 - Bonds.

    2010-04-01

    ... CIVILIZED TRIBES, OKLAHOMA, FOR MINING How to Acquire Leases § 213.15 Bonds. (a) Lessee shall furnish with each mining lease a bond (Form 5-154b), and an assignee of a lease shall furnish with each assignment a... personal sureties and a deposit as collateral security of any public-debt obligations of the United...

  19. 49 CFR 234.213 - Grounds.

    2010-10-01

    ... TRANSPORTATION GRADE CROSSING SIGNAL SYSTEM SAFETY AND STATE ACTION PLANS Maintenance, Inspection, and Testing Maintenance Standards § 234.213 Grounds. Each circuit that affects the proper functioning of a highway-rail... in the circuit. This requirement does not apply to: circuits that include track rail;...

  20. 48 CFR 52.213-2 - Invoices.

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Invoices. 52.213-2 Section... Invoices. As prescribed in 13.302-5(b), insert the following clause: Invoices (APR 1984) The Contractor's invoices must be submitted before payment can be made. The Contractor will be paid on the basis of...

  1. 19 CFR 213.2 - Definitions.

    2010-04-01

    ... UNITED STATES INTERNATIONAL TRADE COMMISSION INVESTIGATIONS OF UNFAIR PRACTICES IN IMPORT TRADE TRADE REMEDY ASSISTANCE § 213.2 Definitions. (a) Office. The Trade Remedy Assistance Office (hereinafter Office... Trade Remedy Assistance Office, U.S. International Trade Commission, 500 E Street SW., Washington,...

  2. 22 CFR 213.15 - Use of credit reporting bureaus.

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Use of credit reporting bureaus. 213.15 Section 213.15 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT CLAIMS COLLECTION Collection § 213.15..., address, taxpayer identification number and any other information necessary to establish the identity...

  3. 22 CFR 213.11 - Aggressive collection actions; documentation.

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Aggressive collection actions; documentation. 213.11 Section 213.11 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT CLAIMS COLLECTION Collection § 213.11 Aggressive collection actions; documentation. (a) USAID takes actions and...

  4. 14 CFR 1274.213 - Distribution of cooperative agreements.

    2010-01-01

    ... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Distribution of cooperative agreements. 1274.213 Section 1274.213 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION COOPERATIVE AGREEMENTS WITH COMMERCIAL FIRMS Pre-Award Requirements § 1274.213 Distribution of...

  5. 49 CFR 229.213 - Locomotive manufacturing information.

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Locomotive manufacturing information. 229.213 Section 229.213 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD... Design Requirements § 229.213 Locomotive manufacturing information. (a) Each railroad operating...

  6. 27 CFR 24.213 - Heavy bodied blending wine.

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Heavy bodied blending wine. 24.213 Section 24.213 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS WINE Production of Other Than Standard Wine § 24.213 Heavy bodied blending wine. Heavy bodied blending wine...

  7. 7 CFR 1280.213 - Investment of funds.

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Investment of funds. 1280.213 Section 1280.213... INFORMATION ORDER Lamb Promotion, Research, and Information Order Expenses § 1280.213 Investment of funds. The... thereof, in any interest-bearing account or certificate of deposit of a financial institution that is...

  8. 5 CFR 213.3102 - Entire executive civil service.

    2010-01-01

    ...) Editorial Note: For Federal Register citations affecting § 213.3102, see the List of CFR Sections Affected... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Entire executive civil service. 213.3102 Section 213.3102 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE...

  9. 47 CFR 36.213 - Network access services revenues.

    2010-10-01

    ... 47 Telecommunication 2 2010-10-01 2010-10-01 false Network access services revenues. 36.213 Section 36.213 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES... Income Accounts Operating Revenues § 36.213 Network access services revenues. (a) End User...

  10. Dicty_cDB: SLG213 [Dicty_cDB

    Full Text Available ycdb.biol.tsukuba.ac.jp/CSM/SL/SLG2-A/SLG213Q.Seq.d/ Representative seq. ID SLG21...3Z (Link to Original site) Representative DNA sequence >SLG213 (SLG213Q) /CSM/SL/SLG2-A/SLG213Q.Seq.d/ XXXXX...lstfqqmwiskeeydesgpsivhrkcf*tiktsdesashtykyi* mynnnki*lflmvvdlyptikkkk Tr...gpsivhrkcf*tiktsdesashtykyi* mynnnki*lflmvvdlyptikkkk Frame C: ---ptspstlklkckllphhqpsliht...M/VF/VFJ6-A/VFJ616Q.Seq.d/ 985 0.0 own update 2004.12.25 Homology vs DNA Score E Sequences producing signifi

  11. Radioimmunotherapy of infection with 213Bi-labeled antibodies

    Dadachova, Ekaterina

    2008-01-01

    Bismuth-213 (213Bi) (physical half-life 46 min) is a beta-emitter (97%) and an alpha-emitter (3%) which decays to short lived alpha-emitter Polonium-213 and could therefore be used as an in vivo generator of alpha particles with the energy of around 8 MeV. 213Bi has been successfully used during the last decade in both clinical and pre-clinical work for radioimmunotherapy (RIT) of cancer with 213Bi-labeled monoclonal antibodies (mAbs). RIT has been proposed as a novel techonology for treatmen...

  12. Groundwater seepage from the Ranger uranium mine tailings dam: radioisotopes of radium, thorium and actinium. Supervising Scientist report 106

    Monitoring of bores near the Ranger uranium mine tailings dam has revealed deterioration in water quality in several bores since 1983. In a group of bores to the north of the dam, increases have been observed of up to 500 times for sulphate concentrations and of up to 5 times for 226Ra concentrations. Results are presented here of measurements of members of the uranium, thorium and actinium decay series in borewater samples collected between 1985 and 1993. In particular, measurements of all four naturally-occurring radium isotopes have been used in an investigation of the mechanism of radium concentration changes. For the most seepage-affected bores the major findings of the study include: 228Ra/226Ra 223Ra /226Ra and 224Ra/228Ra ratios all increased over the course of the study; barium concentrations show high seasonal variability, being lower in November than May, but strontium concentrations show a steady increase with time. Calculations show that the groundwater is probably saturated with respect to barite but not with respect to celestite or anglesite; sulphide concentrations are low in comparison with sulphate, and are higher in November than in May; and 227Ac concentrations have increased with time, but do not account for the high 223Ra/226Ra ratios. It is concluded on the basis of these observations that increases in Ra isotope concentrations observed in a number of seepage-affected bores arise from increases in salinity leading to desorption of radium from adsorption sites in the vicinity of the bore rather by direct transport of radium from the tailings. Increased salinity is also causing the observed increases in 227Ac and strontium concentrations, while formation of a barite solid phase in the groundwater is causing the removal of some radium from solution. This is the cause of the increasing radium isotope ratios noted above

  13. Upgrades of USK 213 and SK 187

    SKODA JS succeeded in improving systems for reactor pressure vessel inspection from the outer surface. All weak points of the original inspection systems were removed. The upgraded systems conform with the demands of codes used presently for the inspection of WWER reactor pressure vessels, and the automated inspection performed using them covers all parts indicated in the pre-service and in-service inspection programmes. All parameters of the improved USK 213 and SK 187 systems are comparable with similar devices used in other countries; the systems are thus fully qualified for future in-service inspections of WWER pressure vessels

  14. 49 CFR 213.9 - Classes of track: operating speed limits.

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Classes of track: operating speed limits. 213.9... speed limits. (a) Except as provided in paragraph (b) of this section and §§ 213.57(b), 213.59(a), 213.113(a), and 213.137(b) and (c), the following maximum allowable operating speeds apply— Over...

  15. 29 CFR 1910.213 - Woodworking machinery requirements.

    2010-07-01

    ... 29 Labor 5 2010-07-01 2010-07-01 false Woodworking machinery requirements. 1910.213 Section 1910..., DEPARTMENT OF LABOR OCCUPATIONAL SAFETY AND HEALTH STANDARDS Machinery and Machine Guarding § 1910.213 Woodworking machinery requirements. (a) Machine construction general. (1) Each machine shall be so...

  16. 45 CFR 213.3 - Use of gender and number.

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false Use of gender and number. 213.3 Section 213.3... gender and number. As used in this part, words importing the singular number may extend and be applied to several persons or things, and vice versa. Words importing the masculine gender may be applied to...

  17. 14 CFR 91.213 - Inoperative instruments and equipment.

    2010-01-01

    ... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Inoperative instruments and equipment. 91.213 Section 91.213 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION... authorization constitute a supplemental type certificate for the aircraft. (3) The approved Minimum...

  18. 14 CFR 135.213 - Weather reports and forecasts.

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Weather reports and forecasts. 135.213... Operating Limitations and Weather Requirements § 135.213 Weather reports and forecasts. (a) Whenever a person operating an aircraft under this part is required to use a weather report or forecast, that...

  19. 40 CFR 262.213 - Laboratory clean-outs.

    2010-07-01

    ... product (listed in 40 CFR part 261, subpart D or exhibiting one or more characteristics in 40 CFR part 261... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Laboratory clean-outs. 262.213 Section....213 Laboratory clean-outs. (a) One time per 12 month period for each laboratory, an eligible...

  20. 24 CFR 213.260 - Allowable methods of premium payment.

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Allowable methods of premium payment. 213.260 Section 213.260 Housing and Urban Development Regulations Relating to Housing and Urban... Allowable methods of premium payment. Premiums shall be payable in cash or in debentures at par plus...

  1. 29 CFR 825.213 - Employer recovery of benefit costs.

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Employer recovery of benefit costs. 825.213 Section 825.213 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR OTHER LAWS... situations as where a parent chooses to stay home with a newborn child who has a serious health condition;...

  2. Dicty_cDB: SLE213 [Dicty_cDB

    Full Text Available SL (Link to library) SLE213 (Link to dictyBase) - G24168 DDB0217029 Contig-U04013-1 SLE213F (Lin ... -Sampling_GS-28-01-01-1... 50 0.001 2 ( EL349336 ) CCE L5739.b1_F20.ab1 CCE (LMS) endive Cichorium end... 4 ... 1_75( CP000581 |pid:none) Ostreococcus lucimarinus CCE 9901 ... 196 7e-49 AK078936_1( AK078936 |pid:none) ...

  3. Dicty_cDB: SHA213 [Dicty_cDB

    Full Text Available SH (Link to library) SHA213 (Link to dictyBase) - - - Contig-U11277-1 SHA213P (Link to Original ... 2 3 AJ242743 |AJ242743.1 Schizosaccharomyces pombe tpp 1+ gene for trehalose-6P phosphatase, exon 1. 50 0. ... hizosaccharomyces pombe phosphatidate phosphatase (TPP ) mRNA, partial cds. 50 0.062 1 D89225 |D89225.1 Sc ...

  4. A process definition repository based on step AP 213

    Butler, J.W.

    1997-09-01

    Over the years, in the context of numerically controlled machined part manufacturing, the loss, misinterpretation, and redundancy of re-inputting manufacturing instructions and data during the evolution of a product design into the finished product has been a resource depleting and costly endeavor. It is the intent of this project to utilize the emerging standards from the International Standards Organization, ISO 10303 Standard for the Exchange of Product Model Data, commonly referred to as STEP, to store and retrieve process planning information for a set of production work instructions. The project focuses on the utilization of the 1995 version of the Draft International Standard ISO/DIS 10303-213:1995 (E) Application protocol: Numerical control process plans for machined parts (AP213). This project illustrates the methodologies used to build an object-oriented Process Definition Repository (PDR), describes both the benefits and shortcomings experienced in implementing AP213, and recommends enhancements to AP213 for process planning information. The deliverable will be a Part 21 data file, based on the application-interpreted model for AP213 and integrated with product design data.

  5. Radioimmunotherapy of micrometastases in lung with vascular targeted213Bi

    Kennel, S. J.; Boll, R.; Stabin, M; Schuller, H M; Mirzadeh, S

    1999-01-01

    A model system has been used to test the efficacy of vascular targeting of α-particle emitter213Bi for therapy of small, ‘artificial’ metastases in mouse lung. Specific monoclonal antibody (mAb) 201B was used to deliver greater than 30% of the injected dose to lung where tumours had developed due to intravenous injection of cells. Specific213Bi-mAb 201B treatment of BALB/c mammary carcinoma EMT-6 tumours in lung resulted in a dose-dependent destruction of tumours and an extended lifespan of t...

  6. 18 CFR 157.213 - Underground storage field facilities.

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Underground storage... of the Natural Gas Act for Certain Transactions and Abandonment § 157.213 Underground storage field... operate facilities for the remediation and maintenance of an existing underground storage...

  7. LabVIEW Data Acquisition for NE213 Neutron Detector

    A neutron spectroscopy system based on a NE213 liquid scintillation detector at the Stanford Linear Accelerator Center measures neutron energies from a few MeV up to 800 MeV. The neutrons are produced from the electron beam and target interactions. The NE 213 scintillator, coupled with a Photomultiplier Tube (PMT), detects and converts radiation into electric pulses for signal processing. Signals are processed through Nuclear Instrument Modules (NIM) and Computer Automated Measurement and Control (CAMAC) modules. The processed pulses are then fed into a CAMAC analog to digital converter module (ADC). The ADC classifies the incoming analog pulses into one of 2048 digital channels. Data acquisition (DAQ) software based on LabVIEW version 7.0 acquires and organizes data from the CAMAC ADC. The DAQ system presents a spectrum showing a relationship between pulse events and respective charge (digital channel number). Various photon sources, such as Co-60, Y-88, and AmBe-241, are used to calibrate the NE213 detector. For each source, a Compton edge and reference energy in MeVee is obtained, resulting in a calibration curve. This project is focused on the development of a DAQ system and control setup to collect and process information from a NE213 liquid scintillation detector. A manual is also created to document the process of the development and interpretation of the LabVIEW-based DAQ system

  8. 19 CFR 10.213 - Articles eligible for preferential treatment.

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Articles eligible for preferential treatment. 10...; DEPARTMENT OF THE TREASURY ARTICLES CONDITIONALLY FREE, SUBJECT TO A REDUCED RATE, ETC. Textile and Apparel Articles Under the African Growth and Opportunity Act § 10.213 Articles eligible for preferential...

  9. 41 CFR 101-29.213 - Commercial product.

    2010-07-01

    ... 41 Public Contracts and Property Management 2 2010-07-01 2010-07-01 true Commercial product. 101... Regulations System FEDERAL PROPERTY MANAGEMENT REGULATIONS SUPPLY AND PROCUREMENT 29-FEDERAL PRODUCT DESCRIPTIONS 29.2-Definitions § 101-29.213 Commercial product. A commercial product is any item, component,...

  10. 22 CFR 213.21 - Employee salary offset-general.

    2010-04-01

    ... administrative offset; (3) 5 CFR part 550, subpart K, setting forth the minimum requirements for executive agency regulations on salary offset; and (4) 31 CFR parts 900 through 904, the Federal Claims Collection Standards. ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Employee salary offset-general. 213.21...

  11. 25 CFR 213.43 - Relinquishment of Government supervision.

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Relinquishment of Government supervision. 213.43 Section... Relinquishment of Government supervision. All oil and gas leases hereafter executed shall contain the following relinquishment of supervision clause and terms operative after such relinquishment, or other provisions...

  12. 12 CFR 213.9 - Relation to state laws.

    2010-01-01

    ... interpretative responsibilities for the state consumer leasing law, may apply to the Board for a preemption... Banks and Banking FEDERAL RESERVE SYSTEM BOARD OF GOVERNORS OF THE FEDERAL RESERVE SYSTEM CONSUMER LEASING (REGULATION M) § 213.9 Relation to state laws. (a) Inconsistent state law. A state law that...

  13. 23 CFR 645.213 - Use and occupancy agreements (permits).

    2010-04-01

    ... OPERATIONS UTILITIES Accommodation of Utilities § 645.213 Use and occupancy agreements (permits). The written... will not be applicable to each individual utility installation, the use and occupancy agreement must..., maintenance, access restriction, and any special conditions applicable to each installation. (b) A...

  14. VERITAS Observations of HESS J1943+213

    ,

    2015-01-01

    HESS J1943+213 is a very-high-energy (VHE; >100 GeV) gamma-ray point source detected during the H.E.S.S. Galactic Plane Survey. Radio, infrared, X-ray, and GeV gamma-ray counterparts have been identified for HESS J1943+213; however, the classification of the source is still uncertain. Recent publications have argued primarily in favor of an extreme BL Lac object behind the Galactic plane, though the scenario that HESS J1943+213 is a young pulsar wind nebula is viable as well. We present deep VERITAS observations of HESS J1943+213, which provide the most significant VHE detection of the source so far, with ~18 sigma excess. The source is detected at ~2% Crab Nebula flux above 200 GeV with VERITAS, with the source spectrum well fit by a power-law function and showing agreement with the H.E.S.S. detection. We also include results from analysis of Swift XRT observations contemporaneous with VERITAS. No significant flux or spectral variability is detected with VERITAS or Swift XRT observations. We place the VERITA...

  15. 8 CFR 213a.2 - Use of affidavit of support.

    2010-01-01

    ... section 203(b) of the Act, if a relative (as defined in 8 CFR 213a.1) of the intending immigrant is a... an alien who is accompanying, as defined in 22 CFR 40.1, a principal intending immigrant must submit... intending immigrant must include with his or her application for an immigrant visa or adjustment of status...

  16. 49 CFR 213.59 - Elevation of curved track; runoff.

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Elevation of curved track; runoff. 213.59 Section... track; runoff. (a) If a curve is elevated, the full elevation shall be provided throughout the curve, unless physical conditions do not permit. If elevation runoff occurs in a curve, the actual...

  17. 9 CFR 113.213 - Pseudorabies Vaccine, Killed Virus.

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Pseudorabies Vaccine, Killed Virus..., DEPARTMENT OF AGRICULTURE VIRUSES, SERUMS, TOXINS, AND ANALOGOUS PRODUCTS; ORGANISMS AND VECTORS STANDARD REQUIREMENTS Killed Virus Vaccines § 113.213 Pseudorabies Vaccine, Killed Virus. Pseudorabies Vaccine,...

  18. 40 CFR 180.213 - Simazine; tolerances for residues.

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Simazine; tolerances for residues. 180... PROGRAMS TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Specific Tolerances § 180.213 Simazine; tolerances for residues. (a) General. Tolerances are established for the combined residues of...

  19. The synthesis of [2-13C]2-nitropropane at room temperature and at atmospheric pressure

    Jacquemijns M; Zomer G

    1990-01-01

    In this report the synthesis of [2-13C]2-nitropropane at room temperature is described. [2-13C]Acetone was converted into the oxime with hydroxy hydrochloridelamine and sodium carbonate. Treatment with hypobromic acid resulted in 2-13C]2-bromo-2-nitropropane. Hydrogenation with sodium borohydride gave [2-13C]2-nitropropane in 14,3% overall yield.

  20. The synthesis of [2-13C]2-nitropropane at room temperature and at atmospheric pressure

    Jacquemijns M; Zomer G

    1990-01-01

    In this report the synthesis of [2-13C]2-nitropropane at room temperature is described. [2-13C]Acetone was converted into the oxime with hydroxy hydrochloridelamine and sodium carbonate. Treatment with hypobromic acid resulted in 2-13C]2-bromo-2-nitropropane. Hydrogenation with sodium borohydride

  1. 49 CFR 213.347 - Automotive or railroad crossings at grade.

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Automotive or railroad crossings at grade. 213.347 Section 213.347 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD... Higher § 213.347 Automotive or railroad crossings at grade. (a) There shall be no at-grade...

  2. 24 CFR 213.276 - Allocation of Cooperative Management Housing Insurance Fund income or losses.

    2010-04-01

    ... Management Housing Insurance Fund income or losses. 213.276 Section 213.276 Housing and Urban Development... Rights and Obligations-Projects Cooperative Management Housing Insurance and Distributive Shares § 213.276 Allocation of Cooperative Management Housing Insurance Fund income or losses. For any...

  3. 48 CFR 213.306 - SF 44, Purchase Order-Invoice-Voucher.

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false SF 44, Purchase Order-Invoice-Voucher. 213.306 Section 213.306 Federal Acquisition Regulations System DEFENSE ACQUISITION... PROCEDURES Simplified Acquisition Methods 213.306 SF 44, Purchase Order-Invoice-Voucher. (a)(1) The...

  4. 46 CFR 14.213 - Report of shipment of merchant mariner.

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Report of shipment of merchant mariner. 14.213 Section 14.213 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN SHIPMENT AND DISCHARGE OF MERCHANT MARINERS Shipment of Merchant Mariners § 14.213 Report of shipment...

  5. LabVIEW DAQ for NE213 Neutron Detector

    A neutron spectroscopy system, based on a NE213 liquid scintillation detector, to be placed at the Stanford Linear Accelerator Center to measure neutron spectra from a few MeV up to 800 MeV, beyond shielding. The NE213 scintillator, coupled with a Photomultiplier Tube (PMT), detects and converts radiation into current for signal processing. Signals are processed through Nuclear Instrument Modules (NIM) and Computer Automated Measurement and Control (CAMAC) modules. CAMAC is a computer automated data acquisition and handling system. Pulses are properly prepared and fed into an analog to digital converter (ADC), a standard CAMAC module. The ADC classifies the incoming analog pulses into 1 of 2048 digital channels. Data acquisition (DAQ) software based on LabVIEW, version 7.0, acquires and organizes data from the CAMAC ADC. The DAQ system presents a spectrum showing a relationship between pulse events and respective charge (digital channel number). Various photon sources, such as Co-60, Y-88, and AmBe-241, are used to calibrate the NE213 detector. For each source, a Compton edge and reference energy [units of MeVee] is obtained. A complete calibration curve results (at a given applied voltage to the PMT and pre-amplification gain) when the Compton edge and reference energy for each source is plotted. This project is focused to development of a DAQ system and control setup to collect and process information from a NE213 liquid scintillation detector. A manual is created to document the process of the development and interpretation of the LabVIEW-based DAQ system. Future high-energy neutron measurements can be referenced and normalized according to this calibration curve

  6. Effect of Fluorination of 2,1,3-Benzothiadiazole

    Nielsen, Christian B.; White, Andrew J P; McCulloch, Iain

    2015-01-01

    The 4,7-dithieno-2,1,3-benzothiadiazole (DTBT) moiety and its fluorinated counterpart are important π-conjugated building blocks in the field of organic electronics. Here we present a combined experimental and theoretical investigation into fundamental properties relating to these two molecular entities and discuss the potential impact on extended π-conjugated materials and their electronic properties. While the fluorinated derivative, in the solid state, packs with a cofacial overlap smaller...

  7. Calibration of a NE213 detector for neutron spectroscopy

    This work describes the experimental way followed for getting the calibration of a NE213 detector with a beam of neutrons from the J.E.N. 2 MeV Van de Graaff and using at once pulse shape discrimination. Detector has been used for measuring the spectrum of the fast reactor CORAL-1. There is also included an experimental method in order to get with precision where the Compton edge is placed on the electron spectrum. (Author) 9 refs

  8. Calibration of a NE213 detector for neutron spectroscopy; Calibracion de un detector de NE213 para espectroscopia de neutrones

    Blazquez Martinez, J.; Butragueno Casado, J. L.

    1974-07-01

    This work describes the experimental way followed for getting the calibration of a NE213 detector with a beam of neutrons from the J.E.N. 2 MeV Van de Graaff and using at once pulse shape discrimination. Detector has been used for measuring the spectrum of the fast reactor CORAL-1. There is also included an experimental method in order to get with precision where the Compton edge is placed on the electron spectrum. (Author) 9 refs.

  9. n-γ discrimination of NE213 scintillator%NE213闪烁体的n-γ分辨

    叶邦角; NanjyoH; KobayashiT; TsunoS; HasukoK

    2003-01-01

    NE213闪烁体广泛使用于探测快中子,但却伴随很高的γ本底.本文使用快信号门与总信号门的两门积分方法,用快信号与总信号进行二维作图来分辨粒子.用252Cf 中子源和d+D核反应产生的单能中子研究了φ5"×2"、φ5"×5"和φ8"×2"三种尺寸的NE213闪烁体的n-γ分辨性能,通过选择合适的门宽,获得了极好的n-γ分辨效果.对φ5"×2"的NE213,最佳快信号门宽为30ns.

  10. Irradiation embrittlement monitoring of WWER-440/213 type RPV's

    The brief results from the surveillance specimen program's application on the RPV's WWER-440/213 in Jaslovske Bohunice V-2 and Mochovce NPP's was finished the standard surveillance specimen program (SSSP). On the base of SSSP critical analysis was prepared so called 'Extended Surveillance Specimen Program' (ESSP). For the first two units of the Mochovce NPP is prepared completely new program of irradiation embrittlement monitoring called 'Modern Surveillance Specimen Program' (MSSP), This program will serve for Mochovce NPP during all planned service life. The first results of ESSP from the 3-rd and 4-th units in Jaslovske Bohunice V-2 NPP are presented too. (author)

  11. Preparation of a 1 mg/cm213C target

    We give a description for the production of 13C foils and the preparation of an approximately 1 mg/cm2 thick target. A recent experiment to measure the mass of 36Ca via the 40Ca(4He,8He)36Ca reaction at the S800 magnetic spectrometer at MSU, needed a precise determination of the beam energy and calibration of the focal plane of the magnetic spectrometer in order to achieve the most accurate value for the 36Ca mass. The 1 mg/cm213C target was essential for these purposes. Target performance along with some preliminary results will also be discussed

  12. A comparative risk assessment for the Russian V213 power reactor

    Marshall, T.D.; Hockenbury, R.W. [Rensselaer Polytechnic Inst., Troy, NY (United States). Dept. of Nuclear Engineering and Engineering Physics; Honey, J.A. [American Nuclear Insurers, Farmington, CT (United States); Cadwallader, L.C. [Idaho National Engineering Lab., Idaho Falls, ID (United States)

    1996-04-01

    Probabilistic risk assessment methodology is applied to generate an evaluation of the relative likelihood of safe recovery following selected pressurized water reactor (PWR) design basis accidents for a Russian V213 nuclear power reactor. US-designed PWRs similar to the V213 are used for reference and comparison. This V213 risk assessment is based on comparison analyses of the following aspects: accident progression event tree success paths for typical PWR accident initiating events, safety aspects in reactor design, and perceived performance of reactor safety systems. The four initiating events considered here are: loss of offsite power with station blackout, large-break loss-of-coolant accident (LOCA), medium-break LOCA, and small-break LOCA. The success probabilities for the V213 reaching a non-core-damage state after the onset of the selected initiating events are calculated for two scenarios: (a) using actual component reliability data from US PWRs and (b) assuming common component reliability data. US PWR component reliability data are used based of the unavailability of such data for the V213 at the time of the analyses. While the use of US PWR data in this risk assessment of the V213 does strongly infer V213 comparability to US plants, the risk assessment using common component reliability does not have such a stringent limitation and is thus a separate scoping assessment of the V213 engineered safety systems. The results of the analyses suggest that the V213 has certain design features that significantly improve the reactor`s safety margin for the selected initiating events and that the V213 design has a relative risk of core damage for selected initiating events that is at least comparable to US PWRs. It is important to realize that these analyses are of a scoping nature and may be significantly influenced by important risk factors such as V213 operator training, quality control, and maintenance procedures.

  13. 24 CFR 213.275 - Nature of the Cooperative Management Housing Insurance Fund.

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Nature of the Cooperative Management Housing Insurance Fund. 213.275 Section 213.275 Housing and Urban Development Regulations Relating... Nature of the Cooperative Management Housing Insurance Fund. The Cooperative Management Housing...

  14. 5 CFR 213.3301 - Positions of a confidential or policy-determining nature.

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Positions of a confidential or policy-determining nature. 213.3301 Section 213.3301 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL... or policy-determining nature. (a) Upon specific authorization by OPM, agencies may make...

  15. 48 CFR 213.270 - Use of the Governmentwide commercial purchase card.

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Use of the Governmentwide commercial purchase card. 213.270 Section 213.270 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACTING METHODS AND CONTRACT TYPES SIMPLIFIED ACQUISITION PROCEDURES Actions at or Below...

  16. 33 CFR 117.213 - New Haven Harbor, Quinnipiac and Mill Rivers.

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false New Haven Harbor, Quinnipiac and Mill Rivers. 117.213 Section 117.213 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF... Haven Harbor, Quinnipiac and Mill Rivers. The draws of the Tomlinson bridge, mile 0.0, the Ferry...

  17. 49 CFR 213.143 - Frog guard rails and guard faces; gage.

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Frog guard rails and guard faces; gage. 213.143... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION TRACK SAFETY STANDARDS Track Structure § 213.143 Frog guard rails and guard faces; gage. The guard check and guard face gages in frogs shall be within the...

  18. 49 CFR 213.355 - Frog guard rails and guard faces; gage.

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Frog guard rails and guard faces; gage. 213.355... Higher § 213.355 Frog guard rails and guard faces; gage. The guard check and guard face gages in frogs... distance between the gage line of a frog to the guard line 1 of its guard rail or guarding face,...

  19. 48 CFR 253.213-70 - Completion of DD Form 1155, Order for Supplies or Services.

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Completion of DD Form 1155... Forms 253.213-70 Completion of DD Form 1155, Order for Supplies or Services. Follow the procedures at PGI 253.213-70 for completion of DD Form 1155....

  20. 22 CFR 213.37 - Referrals to the Department of Justice.

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Referrals to the Department of Justice. 213.37... Department of Justice § 213.37 Referrals to the Department of Justice. (a) The CFO, through the FMS cross... of Justice's Nationwide Central Intake Facility as required by the Claims Collection...

  1. 31 CFR 585.213 - Exemption of activities related to certain international organizations.

    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Exemption of activities related to certain international organizations. 585.213 Section 585.213 Money and Finance: Treasury Regulations... related to certain international organizations. Any activities related to the United Nations...

  2. 19 CFR 213.4 - Disclosure of receipt of technical assistance.

    2010-04-01

    ... or application which is filed with the Commission or any other agency which administers the trade law....4 Section 213.4 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION INVESTIGATIONS OF UNFAIR PRACTICES IN IMPORT TRADE TRADE REMEDY ASSISTANCE § 213.4 Disclosure of receipt of technical assistance....

  3. Pulse-shape discrimination in NE213 liquid scintillator detectors

    Cavallaro, M., E-mail: manuela.cavallaro@lns.infn.it [INFN-Laboratori Nazionali del Sud, Catania (Italy); CSFNSM, Catania (Italy); Tropea, S. [INFN-Laboratori Nazionali del Sud, Catania (Italy); Dipartimento di Fisica e Astronomia, Università di Catania, Catania (Italy); Agodi, C. [INFN-Laboratori Nazionali del Sud, Catania (Italy); Assié, M.; Azaiez, F. [Institut de Physique Nucléaire, Université Paris-Sud-11-CNRS/IN2P3, 91406 Orsay (France); Boiano, C. [INFN - Sezione di Milano, Milano (Italy); Bondì, M.; Cappuzzello, F.; Carbone, D. [INFN-Laboratori Nazionali del Sud, Catania (Italy); Dipartimento di Fisica e Astronomia, Università di Catania, Catania (Italy); De Napoli, M. [INFN - Sezione di Catania, Catania (Italy); Séréville, N. de [Institut de Physique Nucléaire, Université Paris-Sud-11-CNRS/IN2P3, 91406 Orsay (France); Foti, A. [Dipartimento di Fisica e Astronomia, Università di Catania, Catania (Italy); INFN - Sezione di Catania, Catania (Italy); Linares, R. [Instituto de Física da Universidade Federal Fluminense, Rio de Janeiro, Niterói, RJ (Brazil); Nicolosi, D. [INFN-Laboratori Nazionali del Sud, Catania (Italy); Dipartimento di Fisica e Astronomia, Università di Catania, Catania (Italy); Scarpaci, J.A. [Institut de Physique Nucléaire, Université Paris-Sud-11-CNRS/IN2P3, 91406 Orsay (France)

    2013-02-01

    The 16-channel fast stretcher BaFPro module, originally developed for processing signals of Barium Fluoride scintillators, has been modified to make a high performing analog pulse-shape analysis of signals from the NE213 liquid scintillators of the EDEN neutron detector array. The module produces two Gaussian signals, whose amplitudes are proportional to the height of the fast component of the output light and to the total energy deposited into the scintillator, respectively. An in-beam test has been performed at INFN-LNS (Italy) demonstrating a low detection threshold, a good pulse-shape discrimination even at low energies and a wide dynamic range for the measurement of the neutrons energy.

  4. An 225Ac/213Bi generator system for therapeutic clinical applications: construction and operation

    A method for construction and operation of an 225Ac/213Bi generator capable of producing 25-100 mCi of 213Bi suitable for clinical antibody labeling is described. The generator has been designed to have an effective lifetime of several weeks, producing up to six therapeutic doses of radionuclide per day. To date, 57 clinical doses have been prepared and injected into patients using the described 213Bi generator. Factors such as radiation damage, radioprotection, iodide eluate chemistry, radiolabeling chemistry and radionuclidic purity are addressed

  5. Response matrices of NE213 scintillation detectors for neutrons

    Four NE213 detectors of different size have been calibrated at the accelerator facility of the PTB. The response functions were experimentally determined for 33 neutron energies between 1 MeV and 16 MeV and compared with Monte Carlo simulations using the NRESP7 code. The light output functions for recoil protons were found to be significantly different for all detectors even if they were of the same size. The neutron fluence determined on the basis of the response functions calculated with the corresponding light output functions agreed to better than ±2% with reference values if energy independent adjustment factors between 0.98 and 1.03 were applied. The response matrices required for the unfolding of neutron induced pulse height spectra were therefore calculated with the NRESP7 code taking into account the adjustment factors. Similarly, the response matrices for photons were calculated with the EGS4 code, but without any adjustment. Finally, the DIFBAS code was applied for the unfolding of pure neutron- and photon-induced pulse height spectra. The resulting spectral fluences are in reasonable agreement with the results obtained by time-of-flight measurements and by spectrometry with a Ge detector

  6. A clinical study of 213 patients with laryngeal cancer

    Miyahara, Hiroshi [Osaka Prefectural Hospital (Japan); Yane, Katsunari [Nara Medical Univ., Kashihara (Japan); Tsuruta, Yoshihiro [Yao Municipal Hospital, Osaka (Japan); Uemura, Hirokazu [Osaka Prefectural Center for Adult Diseases (Japan)

    2002-07-01

    We retrospectively analyzed 213 patients, 197 men and 14 women aged 40 to 87 years, with laryngeal cancer treated at our department from September 1986 to December 1997. The male to female ratio was about 12:1. Cases included 120 glottic (56.3%), 90 supraglottic (42.3%), and 3 subglottic (1.4%). Radiotherapy for early cases and surgery, mainly total laryngectomy for T3 and T4 were conducted first. The 5-year cause-specific survival by site was 97.6% glottic and 77.5% supraglottic. The five-year survival was 88.6% overall. Local control was 83.8% in glottic T1a and 84.6% in glottic T1b. The 3-year preservation of the larynx was 89.1% in glottic T1a and 92.3% in glottic T1b. Distant metastases were observed in 14 cases, mostly to the lung, bones, and liver. At high risk for distant metastasis were those with supraglottic T3-T4, N1, N2b, or pN2c. (author)

  7. Pharmacokinetics and brain uptake of HIV-1 replication inhibitor DB213 in Sprague-Dawley rats.

    Wang, Qianwen; Zhang, Yufeng; Qian, Shuai; Peng, Shaohong; Zhang, Qian; Wong, Chun-Ho; Chan, H Y Edwin; Zuo, Zhong

    2016-06-01

    The current study aims to investigate the pharmacokinetics and brain uptake of HIV-1 replication inhibitor DB213 via a developed LC/MS/MS analytical method. A sensitive, selective, accurate and reliable LC/MS/MS method for determination and quantification of DB213 in rat plasma and brain was developed and validated. A triple quadrupole mass spectrometer equipped with electrospray ionization (ESI) source was applied for the detection of DB213 and benzamidine (Internal Standard). The analytes were quantified by using multiple reaction monitoring (MRM) mode with m/z 333.4→86.1 and m/z 121.2→104 for DB213 and benzamidine respectively. Chromatographic separation of DB213 and benzamidine was achieved on a SunFire C8 (4.6×250mm, i.d. 5μm) analytical column with gradient elution of a mobile phase consisted of acetonitrile and 20mM ammonium formate buffer (containing 0.5% formic acid). The method achieved good linearity from 1.95∼1000ng/ml (r(2)=0.999) in plasma and 0.98∼125ng/ml (r(2)=0.999) in brain. The validated method was successfully applied to plasma pharmacokinetics (PK) and brain uptake of intravenous administration of DB213 water solution (1mg/kg) to Sprague-Dawley rats. It was found that the area under the plasma concentration-time curve from 0 to 360min (AUC0→360min) was 184422.1±42450.8ngmin/ml and the elimination half-life of DB213 after intravenous administration was 70.9±16.1min. In addition, DB213 has demonstrated a potential to cross the blood-brain barrier via intravenous administration with a brain tissue concentration of 11.3±3.6ng/g peaked at 30min post-dosing. PMID:26999321

  8. Multiple product pathways in photodissociation of nitromethane at 213 nm

    Sumida, Masataka; Kohge, Yasunori; Yamasaki, Katsuyoshi; Kohguchi, Hiroshi, E-mail: kohguchi@hiroshima-u.ac.jp [Department of Chemistry, Graduate School of Science, Hiroshima University, Kagamiyama 1-3-1, Higashi-Hiroshima, Hiroshima 739-8526 (Japan)

    2016-02-14

    In this paper, we present a photodissociation dynamics study of nitromethane at 213 nm in the π → π{sup *} transition. Resonantly enhanced multiphoton ionization spectroscopy and ion-imaging were applied to measure the internal state distributions and state-resolved scattering distributions of the CH{sub 3}, NO(X {sup 2}Π, A {sup 2}Σ{sup +}), and O({sup 3}P{sub J}) photofragments. The rotationally state-resolved scattering distribution of the CH{sub 3} fragment showed two velocity components, of which the slower one decreased the relative intensity as the rotational and vibrational excitations. The translational energy distribution of the faster CH{sub 3} fragment indicated the production of the NO{sub 2} counter-product in the electronic excited state, wherein 1 {sup 2}B{sub 2} was the most probable. The NO(v = 0) fragment exhibited a bimodal translational energy distribution, whereas the NO(v = 1 and 2) fragment exhibited a single translational energy component with a relatively larger internal energy. The translational energy of a portion of the O({sup 3}P{sub J}) photofragment was found to be higher than the one-photon dissociation threshold, indicating the two-photon process involved. The NO(A {sup 2}Σ{sup +}) fragment, which was detected by ionization spectroscopy via the Rydberg ←A {sup 2}Σ{sup +} transition, also required two-photon energy. These experimental data corroborate the existence of competing photodissociation product pathways, CH{sub 3} + NO{sub 2},CH{sub 3} + NO + O,CH{sub 3}O + NO, and CH{sub 3}NO + O, following the π → π{sup *} transition. The origins of the observed photofragments are discussed in this report along with recent theoretical studies and previous dynamics experiments performed at 193 nm.

  9. Multiple product pathways in photodissociation of nitromethane at 213 nm.

    Sumida, Masataka; Kohge, Yasunori; Yamasaki, Katsuyoshi; Kohguchi, Hiroshi

    2016-02-14

    In this paper, we present a photodissociation dynamics study of nitromethane at 213 nm in the π → π(*) transition. Resonantly enhanced multiphoton ionization spectroscopy and ion-imaging were applied to measure the internal state distributions and state-resolved scattering distributions of the CH3, NO(X (2)Π, A (2)Σ(+)), and O((3)PJ) photofragments. The rotationally state-resolved scattering distribution of the CH3 fragment showed two velocity components, of which the slower one decreased the relative intensity as the rotational and vibrational excitations. The translational energy distribution of the faster CH3 fragment indicated the production of the NO2 counter-product in the electronic excited state, wherein 1 (2)B2 was the most probable. The NO(v = 0) fragment exhibited a bimodal translational energy distribution, whereas the NO(v = 1 and 2) fragment exhibited a single translational energy component with a relatively larger internal energy. The translational energy of a portion of the O((3)PJ) photofragment was found to be higher than the one-photon dissociation threshold, indicating the two-photon process involved. The NO(A (2)Σ(+)) fragment, which was detected by ionization spectroscopy via the Rydberg ← A (2)Σ(+) transition, also required two-photon energy. These experimental data corroborate the existence of competing photodissociation product pathways, CH3 + NO2,CH3 + NO + O,CH3O + NO, and CH3NO + O, following the π → π(*) transition. The origins of the observed photofragments are discussed in this report along with recent theoretical studies and previous dynamics experiments performed at 193 nm. PMID:26874485

  10. Multiple product pathways in photodissociation of nitromethane at 213 nm

    Sumida, Masataka; Kohge, Yasunori; Yamasaki, Katsuyoshi; Kohguchi, Hiroshi

    2016-02-01

    In this paper, we present a photodissociation dynamics study of nitromethane at 213 nm in the π → π* transition. Resonantly enhanced multiphoton ionization spectroscopy and ion-imaging were applied to measure the internal state distributions and state-resolved scattering distributions of the CH3, NO(X 2Π, A 2Σ+), and O(3PJ) photofragments. The rotationally state-resolved scattering distribution of the CH3 fragment showed two velocity components, of which the slower one decreased the relative intensity as the rotational and vibrational excitations. The translational energy distribution of the faster CH3 fragment indicated the production of the NO2 counter-product in the electronic excited state, wherein 1 2B2 was the most probable. The NO(v = 0) fragment exhibited a bimodal translational energy distribution, whereas the NO(v = 1 and 2) fragment exhibited a single translational energy component with a relatively larger internal energy. The translational energy of a portion of the O(3PJ) photofragment was found to be higher than the one-photon dissociation threshold, indicating the two-photon process involved. The NO(A 2Σ+) fragment, which was detected by ionization spectroscopy via the Rydberg ←A 2Σ+ transition, also required two-photon energy. These experimental data corroborate the existence of competing photodissociation product pathways, CH3 + NO2,CH3 + NO + O,CH3O + NO, and CH3NO + O, following the π → π* transition. The origins of the observed photofragments are discussed in this report along with recent theoretical studies and previous dynamics experiments performed at 193 nm.

  11. Multiple product pathways in photodissociation of nitromethane at 213 nm

    In this paper, we present a photodissociation dynamics study of nitromethane at 213 nm in the π → π* transition. Resonantly enhanced multiphoton ionization spectroscopy and ion-imaging were applied to measure the internal state distributions and state-resolved scattering distributions of the CH3, NO(X 2Π, A 2Σ+), and O(3PJ) photofragments. The rotationally state-resolved scattering distribution of the CH3 fragment showed two velocity components, of which the slower one decreased the relative intensity as the rotational and vibrational excitations. The translational energy distribution of the faster CH3 fragment indicated the production of the NO2 counter-product in the electronic excited state, wherein 1 2B2 was the most probable. The NO(v = 0) fragment exhibited a bimodal translational energy distribution, whereas the NO(v = 1 and 2) fragment exhibited a single translational energy component with a relatively larger internal energy. The translational energy of a portion of the O(3PJ) photofragment was found to be higher than the one-photon dissociation threshold, indicating the two-photon process involved. The NO(A 2Σ+) fragment, which was detected by ionization spectroscopy via the Rydberg ←A 2Σ+ transition, also required two-photon energy. These experimental data corroborate the existence of competing photodissociation product pathways, CH3 + NO2,CH3 + NO + O,CH3O + NO, and CH3NO + O, following the π → π* transition. The origins of the observed photofragments are discussed in this report along with recent theoretical studies and previous dynamics experiments performed at 193 nm

  12. Long-Term Toxicity of 213Bi-Labelled BSA in Mice.

    Laëtitia Dorso

    Full Text Available Short-term toxicological evaluations of alpha-radioimmunotherapy have been reported in preclinical assays, particularly using bismuth-213 (213Bi. Toxicity is greatly influenced not only by the pharmacokinetics and binding specificity of the vector but also by non-specific irradiation due to the circulating radiopharmaceutical in the blood. To assess this, an acute and chronic toxicity study was carried out in mice injected with 213Bi-labelled Bovine Serum Albumin (213Bi-BSA as an example of a long-term circulating vector.Biodistribution of 213Bi-BSA and 125I-BSA were compared in order to evaluate 213Bi uptake by healthy organs. The doses to organs for injected 213Bi-BSA were calculated. Groups of nude mice were injected with 3.7, 7.4 and 11.1 MBq of 213Bi-BSA and monitored for 385 days. Plasma parameters, including alanine aminotransferase (ALT, aspartate aminotransferase (AST, blood urea nitrogen (BUN and creatinine, were measured and blood cell counts (white blood cells, platelets and red blood cells were performed. Mouse organs were examined histologically at different time points.Haematological toxicity was transient and non-limiting for all evaluated injected activities. At the highest injected activity (11.1 MBq, mice died from liver and kidney failure (median survival of 189 days. This liver toxicity was identified by an increase in both ALT and AST and by histological examination. Mice injected with 7.4 MBq of 213Bi-BSA (median survival of 324 days had an increase in plasma BUN and creatinine due to impaired kidney function, confirmed by histological examination. Injection of 3.7 MBq of 213Bi-BSA was safe, with no plasma enzyme modifications or histological abnormalities.Haematological toxicity was not limiting in this study. Liver failure was observed at the highest injected activity (11.1 MBq, consistent with liver damage observed in human clinical trials. Intermediate injected activity (7.4 MBq should be used with caution because of

  13. Vasorelaxant effects of novel Kv7.4 channel enhancers ML213 and NS15370

    Jepps, Thomas Andrew; Bentzen, B H; Stott, J B;

    2014-01-01

    in both rodent and human blood vessels. Recently, two novel Kv7 channel enhancers have been identified, ML213 and NS15370, that show increased potency, particularly on Kv7.4 channels. The aim of this study was to characterise the effects of these novel enhancers in different rat blood vessels and compare...... them to Kv7 enhancers (S-1, BMS-204352, retigabine) described previously. We also sought to determine the binding sites of the new Kv7 enhancers. KEY RESULTS: Both ML213 and NS15370 relaxed segments of rat thoracic aorta, renal artery and mesenteric artery in a concentration-dependent manner....... In the mesenteric artery ML213 and NS15370 displayed EC50 's of 0.74 μM and 0.026 μM, respectively, which were far lower than other Kv7 enhancers tested. Current-clamp experiments revealed both novel enhancers at low concentrations caused significant hyperpolarisation in mesenteric artery smooth muscle cells...

  14. Key elements of the ageing management of the WWER-440/213 type nuclear power plants

    The owner's intention of WWER-440/213 units in Central Europe is to keep the plants in operation as long as technically feasible and reasonable from business point of view. The preconditions of the long term operation and license renewal are the safety and good plant condition. The past and recent ageing management practices have to ensure the required condition and performance of the essential structures, systems and components. In the paper an overall picture of the long term operation of WWER-440/213 units is given. The review covers ageing of mechanical equipment, building structures and electrical equipment to. Key elements of the ageing management are identified and described. Basic issues of assessment of ageing of essential structures, systems and components, also the issues related to availability of design basis information and lacking environmental qualification are discussed. Reference examples typical for WWER-440/213 plants long term operation are given from Paks Nuclear Power Plant practice. (authors)

  15. Evaluation of complexing properties of chelating agents for the bismuth-213

    The bismuth-213 is an alpha- and beta-emitting radioelement of very short physical half-life (45 min) obtained by means of a (225Ac-213Bi) generator. Given its radiotoxicity, this element presents an interest in radioimmunotherapy (RIT). At present, the DTPA derivatives alone are used in radiolabelling of antibodies for RIT. This study presents the complexing properties of other chelates potentially usable to this goal. Four original chelating agents were synthesized in order to choose the families giving the best results in complexing the 213Bi: the tri-ethylene-tetra-amino-hexa-carboxylic acid (HETA), the 1, 4, 7, 10-tetra-aza-cyclo-dodecane-1, 4, 7, 10-tetraacetic- 1- Gly-L-p-nitro Phe-amide (DOTA-pept), the 1, 4, 8, 11-tetrakis [(S)-2 hydroxy-propyl]-1, 4, 8, 11-tetra-aza-cyclo-tetra-decane (THEOH), and the ethylenediamine di-acetate di-acetamide-bis-thiophenol (EDTA-TH). Given the physical characteristics of 213Bi and the goal of our research, the studies of complexation were conducted by C.C.M. on silica, in highly diluted solution, with fixed chelates-213Bi incubation time (15 min), and variable temperature and concentrations. Analysis of the results have been done by means of a phosphor-imager by measuring the number of pixels associated to every chromatographic spot. The obtained results show that the poly-aza poly-carboxylic derivatives and poly-aza di-thio dicarboxylic derivatives are the only able ones to complex rapidly and quantitatively the 213Bi

  16. Alpha-particle emitting 213Bi-anti-EGFR immunoconjugates eradicate tumor cells independent of oxygenation.

    Christian Wulbrand

    Full Text Available Hypoxia is a central problem in tumor treatment because hypoxic cells are less sensitive to chemo- and radiotherapy than normoxic cells. Radioresistance of hypoxic tumor cells is due to reduced sensitivity towards low Linear Energy Transfer (LET radiation. High LET α-emitters are thought to eradicate tumor cells independent of cellular oxygenation. Therefore, the aim of this study was to demonstrate that cell-bound α-particle emitting (213Bi immunoconjugates kill hypoxic and normoxic CAL33 tumor cells with identical efficiency. For that purpose CAL33 cells were incubated with (213Bi-anti-EGFR-MAb or irradiated with photons with a nominal energy of 6 MeV both under hypoxic and normoxic conditions. Oxygenation of cells was checked via the hypoxia-associated marker HIF-1α. Survival of cells was analysed using the clonogenic assay. Cell viability was monitored with the WST colorimetric assay. Results were evaluated statistically using a t-test and a Generalized Linear Mixed Model (GLMM. Survival and viability of CAL33 cells decreased both after incubation with increasing (213Bi-anti-EGFR-MAb activity concentrations (9.25 kBq/ml-1.48 MBq/ml and irradiation with increasing doses of photons (0.5-12 Gy. Following photon irradiation survival and viability of normoxic cells were significantly lower than those of hypoxic cells at all doses analysed. In contrast, cell death induced by (213Bi-anti-EGFR-MAb turned out to be independent of cellular oxygenation. These results demonstrate that α-particle emitting (213Bi-immunoconjugates eradicate hypoxic tumor cells as effective as normoxic cells. Therefore, (213Bi-radioimmunotherapy seems to be an appropriate strategy for treatment of hypoxic tumors.

  17. Yeast Interacting Proteins Database: YGL187C, YGL213C [Yeast Interacting Proteins Database

    Full Text Available rt; phosphorylated Rows with this bait as bait (1) Rows with this bait as prey (0) YGL213C SKI8 Ski complex com...h this bait as prey (0) Prey ORF YGL213C Prey gene name SKI8 Prey description Ski complex compo...in (YPD) 0 Alternative path with 2 intervening proteins (YPD) 0 IST hit 5 IST hit in the opposite bait/prey orientation - ... ...rt chain; N-terminal 25 residues of precursor are cleaved during mitochondrial import; phosphorylated Rows wit...ochondrial inner membrane electron transport chain; N-terminal 25 residues of precursor are cleaved during mitochondrial impo

  18. Spectroscopy of high spin states in sup(211,212,213)Fr

    The level structures of 211Fr, 212Fr and 213Fr have been observed to high spins, approx. 28(h/2π) (and excitation energies approx. 8 MeV) using a variety of gamma-ray spectroscopic techniques. The structure of these nuclides is discussed in terms of couplings of single particle states through empirical shell model calculations. Good agreement with experiment is obtained. In 212Fr and 213Fr core-excited configurations are required to explain the properties of the highest states. A number of long lived states were observed in each nucleus some of which decay by by enhanced E3 transitions. The E3 transition strengths are discussed

  19. The association between the ring finger protein 213 (RNF213) polymorphisms and moyamoya disease susceptibility: a meta-analysis based on case-control studies.

    Sun, Xun-Sha; Wen, Jun; Li, Jiao-Xing; Lai, Rong; Wang, Yu-Fang; Liu, Hui-Jiao; Sheng, Wen-Li

    2016-06-01

    A number of studies assessed the association of ring finger protein 213 (RNF213) gene polymorphisms with moyamoya disease (MMD), but the results were not entirely consistent. This meta-analysis was performed to explore the relationship between RNF213 polymorphisms and moyamoya disease in Asian population. A systematic search from the PubMed, MEDLINE, EMBASE, ISI web of science, CNKI, China CBM and WANFANG DATA databases was conducted to retrieve published studies until March 2015. Statistical analyses were performed using the STATA12.0 software. Fixed or random effects model, subgroup analysis, sensitivity analysis, and publication bias were used to improve the comprehensive analysis. Eight papers including 904 MMD patients and 2258 controls were recruited in the meta-analysis. rs112735431 was closely associated with the risk of MMD among Asian population in all genetic models (dominant model: OR 103.39, 95 % CI 52.25-204.55, P = 1.69e-40; recessive model: OR 16.45, 95 % CI 6.00-45.10, P = 5.33e-08; additive model: OR 61.49, 95 % CI 22.07-171.33, P = 3.32e-15), especially in the Japanese population. Subgroup analysis revealed highly statistically significant higher risk in the patients with family histories. Although another polymorphism rs148731719 showed no significant association with the MMD, rs138130613 was found to be related to the higher risk in Chinese population (dominant model: OR 8.34, 95 % CI 1.72-40.47, P = 0.008). Our meta-analysis strengthens RNF213 rs112735431 is closely associated with the increased risk of MMD in Japanese, and the screening combined with rs112735431 and rs138130613may improve the detection rate for MMD in China. PMID:26847828

  20. 20 CFR 404.213 - Computation where you are eligible for a pension based on your noncovered employment.

    2010-04-01

    ... pension based on your noncovered employment. 404.213 Section 404.213 Employees' Benefits SOCIAL SECURITY... you are eligible for a pension based on your noncovered employment. (a) When applicable. Except as... entitled to a monthly pension(s) for which you first became eligible after 1985 based in whole or part...

  1. 24 CFR 213.259a - Premiums-mortgages insured pursuant to section 238(c) of the Act.

    2010-04-01

    ... HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES COOPERATIVE HOUSING MORTGAGE INSURANCE Contract Rights.... All of the provisions of §§ 213.253 through 213.259 governing mortgage insurance premiums shall...

  2. 22 CFR 213.16 - Use and disclosure of mailing addresses.

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Use and disclosure of mailing addresses. 213.16....16 Use and disclosure of mailing addresses. (a) When attempting to locate a debtor in order to... Revenue Service. (b) Addresses obtained from the Internal Revenue Service will be used by the Agency,...

  3. 48 CFR 1352.213-70 - Evaluation utilizing simplified acquisition procedures.

    2010-10-01

    ... Clauses 1352.213-70 Evaluation utilizing simplified acquisition procedures. As prescribed in 48 CFR 1313.106-2-70, insert the following provision: Evaluation Utilizing Simplified Acquisition Procedures (APR... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Evaluation...

  4. Experimental Treatment of Bladder Cancer with Bi-213-anti-EGFR MAb

    Therapy of non-muscle-invasive bladder cancer (carcinoma in situ) comprises transurethral resection of the tumour and subsequent instillation of the chemotherapeutic drug mitomycin C in order to eradicate remaining tumour cells. Yet 15 – 40% of treated patients relapse within 5 years. Therefore, new therapeutic strategies to combat tumour recurrence are needed. Alpha-particle emitting radionuclides efficiently kill single tumour cells or small tumour cell clusters. Because the epidermal growth factor receptor (EGFR) is overexpressed on bladder cancer cells, conjugates composed of the alpha-emitter Bi-213 and the anti-EGFR antibody matuzumab should provide a powerful drug to eliminate disseminated bladder cancer cells. Therefore, the aims of our study were (i) to analyse the cytotoxic effects of Bi-213-anti-EGFR radioimmunoconjugates at the cellular level, (ii) to evaluate therapeutic efficacy of intravesically applied Bi-213- anti-EGFR-Mab in a nude mouse model with intravesical human bladder cancer xenografts, (iii) to compare Bi- 213-anti-EGFR-Mab efficacy with chemotherapy using mitomycin C and (iv) to demonstrate that radioimmunotherapy is not toxic to cells of the bladder wall and of the kidneys

  5. 16 CFR 1500.213 - Presentation of views under section 7 of the act.

    2010-01-01

    ... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Presentation of views under section 7 of the... REGULATIONS § 1500.213 Presentation of views under section 7 of the act. (a) Presentation of views under..., such guaranty or undertaking, or a verified copy thereof, shall be made a part of such presentation...

  6. Safety assessment of unit 5 (WWER-440/W-213) of the Greifswald nuclear power station

    The report represents the common results of the program of German-Soviet cooperation in reactor safety and radiation protection. The technical plant and features of type WWER-440/W-213 nuclear power plants, basic legal licensing principles, reactor core and pressurized components, load resulting from accidents, systems engineering, spreading impacts, civil engineering aspects, and the evaluation of operating experience are described. (DG)

  7. Direct measurements of secondary water inventory of steam generator PGV-213 in operation

    Tarankov, G.A.; Trunov, N.B.; Dranchenko, B.N.; Kamiagin, W.W. [OKB Gidropress (Russian Federation)

    1997-12-31

    Results of weight measurement of PGV-213 steam generator during filling in, heating-up and power increase are described. Special measurement system based on stress gauges has been developed. Method of derivation of secondary water inventory is described. Comparison of the data for two steam generators prove accuracy of the measurements. (orig.). 1 refs.

  8. 50 CFR 660.213 - Fixed gear fishery-recordkeeping and reporting.

    2010-10-01

    ... 50 Wildlife and Fisheries 9 2010-10-01 2010-10-01 false Fixed gear fishery-recordkeeping and... West Coast Groundfish-Limited Entry Fixed Gear Fisheries § 660.213 Fixed gear fishery—recordkeeping and..., apply to limited entry fixed gear fishery vessels. (b) Declaration reports for limited entry fixed...

  9. 25 CFR 213.34 - Inspection of premises; books and accounts.

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Inspection of premises; books and accounts. 213.34... premises; books and accounts. Lessees shall agree to allow the lessors and their agents or any authorized... books and records, showing manner of operations and persons interested, shall be open at all times...

  10. 48 CFR 1852.213-71 - Evaluation-Other Than Commercial Items.

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Evaluation-Other Than... Provisions and Clauses 1852.213-71 Evaluation—Other Than Commercial Items. As prescribed in 1813.302-570(b) insert the following provision: Evaluation—Other Than Commercial Items—Jun 2002 (a) The Government...

  11. 48 CFR 52.213-4 - Terms and Conditions-Simplified Acquisitions (Other Than Commercial Items)

    2010-10-01

    ... Federal Register citations affecting 52.213-4, see the List of CFR Sections Affected, which appears in the...: (1) The clauses listed below implement provisions of law or Executive order: (i) 52.222-19, Child...-consuming products listed in the ENERGY STAR® Program or Federal Energy Management Program (FEMP) will...

  12. 27 CFR 25.213 - Beer returned to brewery other than that from which removed.

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Beer returned to brewery... AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS BEER Beer Returned to Brewery § 25.213 Beer returned to brewery other than that from which removed. (a) Refund or adjustment of...

  13. Preclinical safety studies of 213BI labeled PA12 for targeted alpha therapy of cancer

    Full text: The urokinase plasminogen activator (uPA) system is involved in cancer growth and metastasis. The plasminogen activator inhibitor type 2 (PAI2) and uPA can form a stable complex, which is bound to the cell surface uPA receptor (uPAR). We labeled PAI2 with 213Bi to form the alpha conjugate. This conjugate targets uPA/uPAR and has been found to have promising therapeutic properties for breast, prostate and pancreatic cancer. This paper reports studies of the acute and delayed toxicity in mice; the effect of lysine renal protection; pharmacokinetics; a comparison of CHX-A-DTPA and cDTPA chelators, and in vivo 213Bi-PAI2 stability by Ca-DTPA challenge. Pharmacokinetics of 213Bi-PAI2 in nude mice demonstrated that the kidneys were the critical organs for retention of Bismuth in the chelate complex. The CHX-A-DTPA and cDTPA immunoconjugates were found to have similar percent I D/kg in the kidney, with no significant retention of Bi evident in other organs such as liver, heart, lung, and spleen. Ca-DTPA chelators significantly reduced the renal 213Bi accumulation at 15 minutes, not for 30 and 120 minutes, indicating high in vivo stability of 213Bi-PAI2 and the need for pre-injection purification of conjugates. The acute toxicity limit by weight loss was more than 450 MBq/kg. Mild to moderate, patchy tubular necrosis was observed accompanied by slight urea increase. Radiation nephritis was the source of lethal delayed toxicity arising at 20 - 30 weeks post-treatment, for which the maximum tolerance dose was 110 MBq/kg. Kidney uptake was not significantly decreased by lysine at 185 MBq/kg, nor was there any change in delayed toxicity

  14. Radioimmunotherapy of fungal infection with 213-Bi- and 188-Re-labeled antibody

    Aim: Radioimmunotherapy (RIT) is a therapeutic modality that utilizes monoclonal antibodies (mAb) radiolabeled with therapeutic radioisotopes to selectively deliver lethal doses of radiation to cells. We hypothesized that 18B7 mAb (murine IgG1), specific for Cryptoccocus neoformans (CN) polysaccharide capsule, may be used to deliver fungicidal doses of radioisotopes to the sites of CN infection in vitro and in vivo. Materials and Methods: 18B7 mAb was radiolabeled with either the beta-emitter 188-Rhenium (188Re) or with the alpha-emitter 213-Bismuth (213Bi). The biodistribution of radiolabeled 18B7 was measured in BALB/c mice with and without intratracheal CN infection. For in vitro killing assays 105 CN cells/well were treated with 0-3.2 μCi 213Bi-18B7 (3 h incubation), 32 μCi 188Re-18B7 (48 h incubation) or with radiolabeled IgG1 MOPC21 as a control and minimal inhibitory concentrations (MIC) were determined. To compare the activity of radiolabeled mAb's against CN infection with an established antifungal drug, we evaluated the susceptibility of CN strain to Amphoterecin B. In vivo therapy of CN was conducted in groups of 10 A/JCr mice infected intravenously with 105 CN cells 24 h prior to treatment with 50-200 μCi 213Bi- or 188Re-18B7, 213Bi- or 188Re-MOPC21, unlabeled 18B7 or saline. Student's t test for unpaired data was used to analyze in vitro data, and log-rank test - for animal survival data. Results: MAb 18B7 preserved its immunoreactivity post-labeling and delivered 10% ID/g to the lungs of the CN-infected BALB/c mice in 24 h after injection. Two-log reduction in colony forming units (CFU) was achieved in treatment of CN with 213Bi-18B7 and 188Re-18B7, which compared favorably with Amphoterecin B. MIC's were determined to be 0.4 μCi/1.5 mg and 4 μCi/1.25 mg mAb for 213Bi-18B7 and 188Re-18B7, respectively. The fungicidal activity of irrelevant mAb 213Bi-or 188Re-MOPC21 was negligible (P213Bi-18B7 and of 100 μCi 188Re-18B7 significantly (P<0

  15. {sup 177}Lu-immunotherapy of experimental peritoneal carcinomatosis shows comparable effectiveness to {sup 213}Bi-immunotherapy, but causes toxicity not observed with {sup 213}Bi

    Seidl, Christof; Zoeckler, Christine; Beck, Roswitha; Senekowitsch-Schmidtke, Reingard [Technische Universitaet Muenchen, Department of Nuclear Medicine, Munich (Germany); Quintanilla-Martinez, Leticia [Universitaetsklinikum Tuebingen, Institute for Pathology, Tuebingen (Germany); Bruchertseifer, Frank [Institute for Transuranium Elements, European Commission, Joint Research Centre, Karlsruhe (Germany)

    2011-02-15

    {sup 213}Bi-d9MAb-immunoconjugates targeting gastric cancer cells have effectively cured peritoneal carcinomatosis in a nude mouse model following intraperitoneal injection. Because the {beta}-emitter {sup 177}Lu has proven to be beneficial in targeted therapy, {sup 177}Lu-d9MAb was investigated in this study in order to compare its therapeutic efficacy and toxicity with those of {sup 213}Bi-d9MAb. Nude mice were inoculated intraperitoneally with HSC45-M2 gastric cancer cells expressing d9-E-cadherin and were treated intraperitoneally 1 or 8 days later with different activities of specific {sup 177}Lu-d9MAb immunoconjugates targeting d9-E-cadherin or with nonspecific {sup 177}Lu-d8MAb. Therapeutic efficacy was evaluated by monitoring survival for up to 250 days. For evaluation of toxicity, both biodistribution of {sup 177}Lu-d9MAb and blood cell counts were determined at different time points and organs were examined histopathologically. Treatment with {sup 177}Lu-immunoconjugates (1.85, 7.4, 14.8 MBq) significantly prolonged survival. As expected, treatment on day 1 after tumour cell inoculation was more effective than treatment on day 8, and specific {sup 177}Lu-d9MAb conjugates were superior to nonspecific {sup 177}Lu-d8MAb. Treatment with 7.4 MBq of {sup 177}Lu-d9MAb was most successful, with 90% of the animals surviving longer than 250 days. However, treatment with therapeutically effective activities of {sup 177}Lu-d9MAb was not free of toxic side effects. In some animals lymphoblastic lymphoma, proliferative glomerulonephritis and hepatocarcinoma were seen but were not observed after treatment with {sup 213}Bi-d9MAb at comparable therapeutic efficacy. The therapeutic efficacy of {sup 177}Lu-d9MAb conjugates in peritoneal carcinomatosis is impaired by toxic side effects. Because previous therapy with {sup 213}Bi-d9MAb revealed comparable therapeutic efficacy without toxicity it should be preferred for the treatment of peritoneal carcinomatosis. (orig.)

  16. 177Lu-immunotherapy of experimental peritoneal carcinomatosis shows comparable effectiveness to 213Bi-immunotherapy, but causes toxicity not observed with 213Bi

    213Bi-d9MAb-immunoconjugates targeting gastric cancer cells have effectively cured peritoneal carcinomatosis in a nude mouse model following intraperitoneal injection. Because the β-emitter 177Lu has proven to be beneficial in targeted therapy, 177Lu-d9MAb was investigated in this study in order to compare its therapeutic efficacy and toxicity with those of 213Bi-d9MAb. Nude mice were inoculated intraperitoneally with HSC45-M2 gastric cancer cells expressing d9-E-cadherin and were treated intraperitoneally 1 or 8 days later with different activities of specific 177Lu-d9MAb immunoconjugates targeting d9-E-cadherin or with nonspecific 177Lu-d8MAb. Therapeutic efficacy was evaluated by monitoring survival for up to 250 days. For evaluation of toxicity, both biodistribution of 177Lu-d9MAb and blood cell counts were determined at different time points and organs were examined histopathologically. Treatment with 177Lu-immunoconjugates (1.85, 7.4, 14.8 MBq) significantly prolonged survival. As expected, treatment on day 1 after tumour cell inoculation was more effective than treatment on day 8, and specific 177Lu-d9MAb conjugates were superior to nonspecific 177Lu-d8MAb. Treatment with 7.4 MBq of 177Lu-d9MAb was most successful, with 90% of the animals surviving longer than 250 days. However, treatment with therapeutically effective activities of 177Lu-d9MAb was not free of toxic side effects. In some animals lymphoblastic lymphoma, proliferative glomerulonephritis and hepatocarcinoma were seen but were not observed after treatment with 213Bi-d9MAb at comparable therapeutic efficacy. The therapeutic efficacy of 177Lu-d9MAb conjugates in peritoneal carcinomatosis is impaired by toxic side effects. Because previous therapy with 213Bi-d9MAb revealed comparable therapeutic efficacy without toxicity it should be preferred for the treatment of peritoneal carcinomatosis. (orig.)

  17. Importance of RNF213 polymorphism on clinical features and long-term outcome in moyamoya disease.

    Kim, Eun-Hee; Yum, Mi-Sun; Ra, Young-Shin; Park, Jun Bum; Ahn, Jae Sung; Kim, Gu-Hwan; Goo, Hyun Woo; Ko, Tae-Sung; Yoo, Han-Wook

    2016-05-01

    OBJECT Moyamoya disease (MMD) is an idiopathic cerebrovascular occlusive disorder prevalent in East Asia. In the pathogenesis of MMD, the important role of genetic factors is being elucidated, and RNF213 has recently been identified as a susceptibility gene for MMD. The aim of this retrospective study was to investigate the RNF213 genotype in patients with MMD and to determine their genotype-phenotype associations. METHODS The study involved 165 Korean MMD patients from 155 unrelated families who were diagnosed with MMD at a single center from 1995 to 2013. Their demographic, radiological, and clinical findings were evaluated. Direct sequencing of the major RNF213 single nucleotide polymorphisms was performed. The association of the common RNF213 variant with MMD risk was evaluated using historical controls for comparison. Correlations between RNF213 genotype and phenotype were statistically analyzed. RESULTS The c.14429G>A (p.R4810K) variant was identified in 125 (75.8%) of 165 MMD patients. Most patients (112) were heterozygous, and 13 patients had 2 copies of the c.14429G>A variant. A novel heterozygous variant, c.12086A>G (p.Q4029R), was found in 1 additional patient. The minor allele frequency of the c.14429G>A variant was significantly higher in the MMD group (138 [41.8%] of 330 patients) than in the control group (8 [1.36%] of 588 subjects; p A (p.R4810K) variant significantly increased the risk of MMD in Korean patients, with an OR of 52.11 (p A (p.R4810K) genotypes occurred more frequently in patients with a family history of MMD. The homozygous variant was highly associated with early-onset MMD (age at onset cerebral infarction at diagnosis, and cognitive impairment in long-term outcome. CONCLUSIONS The findings indicate that the c.14429G>A (p.R4810K) allele of RNF213 is strongly associated with Korean patients with MMD. The homozygous c.14429G>A (p.R4810K) variant is particularly related to early-onset MMD, severe symptomatic manifestations at diagnosis

  18. Bismuth 213-labeled anti-CD45 radioimmunoconjugate to condition dogs for nonmyeloablative allogeneic marrow grafts

    Sandmaier, B M.(Fred Hutchinson Cancer Research Center, Seattle, WA); Bethge, W A.(Fred Hutchinson Cancer Research Center, Seattle, WA); Wilbur, D. Scott (Washington, Univ Of); Hamlin, Donald K.(Washington, Univ Of); Santos, E B.(Fred Hutchinson Cancer Research Center, Seattle, WA); Brechbiel, M W.(National Cancer Institute, National Institutes of Health, Bethesda, MD); Fisher, Darrell R.(BATTELLE (PACIFIC NW LAB)); Storb, R. (Fred Hutchinson Cancer Research Center)

    2002-01-01

    To lower treatment-related mortality and toxicity of conventional marrow transplantation, a nonmyeloablative regimen using 200 cGy total-body irradiation (TBI) and mycophenolate mofetil (MMF) combined with cyclosporine (CSP) for postgrafting immunosuppression was developed. To circumvent possible toxic effects of external- beam gamma irradiation, strategies for targeted radiation therapy were investigated. We tested whether the short-lived (46 minutes) alpha-emitter Bi-213 conjugated to an anti-CD45 monoclonal antibody (mAb) could replace 200 cGy TBI and selectively target hematopoietic tissues in a canine model of nonmyeloablative DLA-identical marrow transplantation. Biodistribution studies using iodine 123-labeled anti-CD45 mAb showed uptake in blood, marrow, lymph nodes, spleen, and liver. In a dose-escalation study, 7 dogs treated with the Bi-213-anti-CD45 conjugate (Bi-213 dose, 0.1-5.9 mCi/kg[3.7-218 MBq/kg]) without marrow grafts had no toxic effects other than a mild, reversible suppression of blood counts. On the basis of these studies, 3 dogs were treated with 0.5 mg/kg Bi-213-labeled anti-CD45 mAb (Bi-213 doses, 3.6, 4.6, and 8.8 mCi/kg[133, 170, and 326 MBq/kg]) given in 6 injections 3 and 2 days before grafting of marrow from DLA-identical littermates. The dogs also received MMF (10 mg/kg subcutaneously twice daily the day of transplantation until day 27 afterward) and CSP (15 mg/kg orally twice daily the day before transplantation until 35 days afterward). Therapy was well tolerated except for transient elevations in levels of transaminases in 3 dogs, followed by, in one dog, ascites. All dogs achieved prompt engraftment and stable mixed hematopoietic chimerism, with donor contributions ranging from 30% to 70% after more than 27 weeks of follow-up. These results form the basis for additional studies in animals and the design of clinical trials using Bi-213 as a nonmyeloablative conditioning regimen with minimal toxicity.

  19. EVN Observations of HESS J1943+213: Evidence for an Extreme TeV BL Lac Object

    Akiyama, Kazunori; Stawarz, Łukasz; Tanaka, Yasuyuki T.; Nagai, Hiroshi; Giroletti, Marcello; Honma, Mareki

    2016-01-01

    We report on the 1.6 GHz (18 cm) VLBI observations of the unresolved, steady TeV source HESS J1943+213 located in the Galactic plane, performed with the European VLBI Network (EVN) in 2014. Our new observations with a nearly full EVN array provide the deepest image of HESS J1943+213 at the highest resolution ever achieved, enabling us to resolve the long-standing issues of the source identification. The milliarcsecond-scale structure of HESS J1943+213 has a clear asymmetric morphology, consis...

  20. Response resolution of large volume cylindrical NE213 liquid scintillators for 0.3-0.9 MeV electrons

    This paper reports on the response resolution of large volume NE213 scintillator measured for electrons with energies from 0.3 to 0.9 MeV. Measurements are being reported for the first time for a 125 mm diameter NE213 detector, using Gamma-Gamma coincidence technique. The NE213 cell was directly mounted on a photomultiplier tube. The results show that response resolution of the liquid scintillator, directly mounted on the photomultiplier tube, does not improve if one include a light guide in the scintillator-photomultiplier coupling

  1. On timing response improvement of an NE213 scintillator attached to two PMTs

    A 5 cm diameter by 6 cm height NE213 scintillator attached to two XP2282 PHOTONIS photomultiplier tubes (PMTs) exposed to 241Americium–Berylium (Am–Be) neutron–gamma source has been used for timing response studies. The neutron–gamma discrimination (NGD) measurements based on a modified zero-crossing (ZC) method show that the discrimination quality, usually expressed in figure-of-merit (FoM) and peak-to-valley (P/V) values, has been improved. The timing response evaluated with Monte Carlo light transport code, PHOTRACK, also verifies this improvement. - Highlights: • An NE213 scintillator attached to two photomultiplier tubes (PMTs) has been proposed. • The neutron–gamma discrimination (NGD) quality factors have been obtained. • The results confirm that the NGD quality of the proposed assembly has been improved

  2. Assessment of computer codes for VVER-440/213-type nuclear power plants

    Szabados, L.; Ezsol, Gy.; Perneczky [Atomic Energy Research Institute, Budapest (Hungary)

    1995-09-01

    Nuclear power plant of VVER-440/213 designed by the former USSR have a number of special features. As a consequence of these features the transient behaviour of such a reactor system should be different from the PWR system behaviour. To study the transient behaviour of the Hungarian Paks Nuclear Power Plant of VVER-440/213-type both analytical and experimental activities have been performed. The experimental basis of the research in the PMK-2 integral-type test facility , which is a scaled down model of the plant. Experiments performed on this facility have been used to assess thermal-hydraulic system codes. Four tests were selected for {open_quotes}Standard Problem Exercises{close_quotes} of the International Atomic Energy Agency. Results of the 4th Exercise, of high international interest, are presented in the paper, focusing on the essential findings of the assessment of computer codes.

  3. Determination of the half-life of 213Fr with high precision

    Fisichella, M.; Musumarra, A.; Farinon, F.; Nociforo, C.; Del Zoppo, A.; Figuera, P.; La Cognata, M.; Pellegriti, M. G.; Scuderi, V.; Torresi, D.; Strano, E.

    2013-07-01

    High-precision measurement of half-life and Qα value of neutral and highly charged α emitters is a major subject of investigation currently. In this framework, we recently pushed half-life measurements of neutral emitters to a precision of a few per mil. This result was achieved by using different techniques and apparatuses at Istituto Nazionale di Fisica Nucleare Laboratori Nazionali del Sud (INFN-LNS) and GSI Darmstadt. Here we report on 213Fr half-life determination [T1/2(213Fr) = 34.14±0.06 s] at INFN-LNS, detailing the measurement protocol used. Direct comparison with the accepted value in the literature shows a discrepancy of more than three sigma. We propose this new value as a reference, discussing previous experiments.

  4. Transcriptome profile of a bovine respiratory disease pathogen: Mannheimia haemolytica PHL213

    2012-01-01

    Background Computational methods for structural gene annotation have propelled gene discovery but face certain drawbacks with regards to prokaryotic genome annotation. Identification of transcriptional start sites, demarcating overlapping gene boundaries, and identifying regulatory elements such as small RNA are not accurate using these approaches. In this study, we re-visit the structural annotation of Mannheimia haemolytica PHL213, a bovine respiratory disease pathogen. M. haemolytica is on...

  5. Experimental evaluation of the seismic capacity of VVER 440-213 type reactor control rod system

    The experimental evaluation of the WWER-440/213 control rod drive seismic capacity was carried out on the CKTI-VIBROSEISM Horizontal Shaking Table, specifically designed for seismic testing of the full-scale control rod drive of WWER-440 and WWER-1000 reactors. A detailed description is given of the experimental conditions and the methodology used. The results are compared with technical demands on control rods in emergency situations. (Z.S.) 4 refs

  6. The 1:1 adduct of caffeine and 2-(1,3-dioxoisoindolin-2-ylacetic acid

    Moazzam H. Bhatti

    2011-09-01

    Full Text Available In the crystal structure of the title adduct [systematic name: 2-(1,3-dioxoisoindolin-2-ylacetic acid–1,3,7-trimethyl-1,2,3,6-tetrahydro-7H-purine-2,6-dione (1/1], C8H10N4O2·C10H7NO4, the components are linked by an O—H...N hydrogen-bond and no proton transfer occurs.

  7. A digital waveform discrimination system for an NE213 scintillator using a VXIbus general purpose waveform digitizer

    We describe the performance of the waveform analyzer Tektronix TVS645 operated with a UNIX based VXIbus system controller, and then report the result of getting the best FOM for neutron measurement with NE213 by using these hardwares

  8. VP16-213 in combined modality treatment of small cell carcinoma of the lung.

    Newman, S B; Bitran, J D; Golomb, H M; Hoffman, P C; DeMeester, T R; Raghavan, V

    1982-04-01

    Thirty-four previously untreated patients with histologically proven small cell carcinoma of the lung were treated with a combined modality therapy program that incorporated VP16-213, an epipodophyllotoxin derivative, into the chemotherapy regimen. Initial therapy for two cycles was with V-CAM, VP16-213, cyclophosphamide, doxorubicin and methotrexate. Following two cycles of V-CAM each patient received radiation therapy consisting of 4000 rads to the primary site, both hila and the mediastinum, as well as 2000 rads as prophylaxis to the whole brain. After a one-week rest period the patients received monthly cycles of V-CAM until death. Of 10 patients with stage IIIM0 disease, 7 had a complete response (CR), 1 a partial response (PR) and 2 had progressive disease. The median survival was still not reached by approximately 18 months. Of 24 patients with supraclavicular and/or metastatic disease there were only 5 patients with a CR, 11 with a PR and 8 with progressive disease. Their median survival was approximately 9 months. The 70% overall response rate and 9.3-month median survival of the entire group are essentially the same results as those in previously reported studies. There appears to be no additional benefit when VP16-213 is incorporated into our combined modality program. PMID:6288390

  9. The moyamoya disease susceptibility variant RNF213 R4810K (rs112735431) induces genomic instability by mitotic abnormality

    Hitomi, Toshiaki [Department of Health and Environmental Sciences, Graduate School of Medicine, Kyoto University, Kyoto (Japan); Habu, Toshiyuki [Radiation Biology Center, Kyoto University, Kyoto (Japan); Kobayashi, Hatasu; Okuda, Hiroko; Harada, Kouji H. [Department of Health and Environmental Sciences, Graduate School of Medicine, Kyoto University, Kyoto (Japan); Osafune, Kenji [Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto (Japan); Taura, Daisuke; Sone, Masakatsu [Department of Medicine and Clinical Science, Graduate School of Medicine, Kyoto University, Kyoto (Japan); Asaka, Isao; Ameku, Tomonaga; Watanabe, Akira; Kasahara, Tomoko; Sudo, Tomomi; Shiota, Fumihiko [Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto (Japan); Hashikata, Hirokuni; Takagi, Yasushi [Department of Neurosurgery, Graduate School of Medicine, Kyoto University, Kyoto (Japan); Morito, Daisuke [Faculty of Life Sciences, Kyoto Sangyo University, Kyoto (Japan); Miyamoto, Susumu [Department of Neurosurgery, Graduate School of Medicine, Kyoto University, Kyoto (Japan); Nakao, Kazuwa [Department of Medicine and Clinical Science, Graduate School of Medicine, Kyoto University, Kyoto (Japan); Koizumi, Akio, E-mail: koizumi.akio.5v@kyoto-u.ac.jp [Department of Health and Environmental Sciences, Graduate School of Medicine, Kyoto University, Kyoto (Japan)

    2013-10-04

    Highlights: •Overexpression of RNF213 R4810K inhibited cell proliferation. •Overexpression of RNF213 R4810K had the time of mitosis 4-fold and mitotic failure. •R4810K formed a complex with MAD2 more readily than wild-type. •iPSECs from the MMD patients had elevated mitotic failure compared from the control. •RNF213 R4810K induced mitotic abnormality and increased risk of aneuploidy. -- Abstract: Moyamoya disease (MMD) is a cerebrovascular disease characterized by occlusive lesions in the Circle of Willis. The RNF213 R4810K polymorphism increases susceptibility to MMD. In the present study, we characterized phenotypes caused by overexpression of RNF213 wild type and R4810K variant in the cell cycle to investigate the mechanism of proliferation inhibition. Overexpression of RNF213 R4810K in HeLa cells inhibited cell proliferation and extended the time of mitosis 4-fold. Ablation of spindle checkpoint by depletion of mitotic arrest deficiency 2 (MAD2) did not shorten the time of mitosis. Mitotic morphology in HeLa cells revealed that MAD2 colocalized with RNF213 R4810K. Immunoprecipitation revealed an RNF213/MAD2 complex: R4810K formed a complex with MAD2 more readily than RNF213 wild-type. Desynchronized localization of MAD2 was observed more frequently during mitosis in fibroblasts from patients (n = 3, 61.0 ± 8.2%) compared with wild-type subjects (n = 6, 13.1 ± 7.7%; p < 0.01). Aneuploidy was observed more frequently in fibroblasts (p < 0.01) and induced pluripotent stem cells (iPSCs) (p < 0.03) from patients than from wild-type subjects. Vascular endothelial cells differentiated from iPSCs (iPSECs) of patients and an unaffected carrier had a longer time from prometaphase to metaphase than those from controls (p < 0.05). iPSECs from the patients and unaffected carrier had significantly increased mitotic failure rates compared with controls (p < 0.05). Thus, RNF213 R4810K induced mitotic abnormalities and increased risk of genomic instability.

  10. The moyamoya disease susceptibility variant RNF213 R4810K (rs112735431) induces genomic instability by mitotic abnormality

    Highlights: •Overexpression of RNF213 R4810K inhibited cell proliferation. •Overexpression of RNF213 R4810K had the time of mitosis 4-fold and mitotic failure. •R4810K formed a complex with MAD2 more readily than wild-type. •iPSECs from the MMD patients had elevated mitotic failure compared from the control. •RNF213 R4810K induced mitotic abnormality and increased risk of aneuploidy. -- Abstract: Moyamoya disease (MMD) is a cerebrovascular disease characterized by occlusive lesions in the Circle of Willis. The RNF213 R4810K polymorphism increases susceptibility to MMD. In the present study, we characterized phenotypes caused by overexpression of RNF213 wild type and R4810K variant in the cell cycle to investigate the mechanism of proliferation inhibition. Overexpression of RNF213 R4810K in HeLa cells inhibited cell proliferation and extended the time of mitosis 4-fold. Ablation of spindle checkpoint by depletion of mitotic arrest deficiency 2 (MAD2) did not shorten the time of mitosis. Mitotic morphology in HeLa cells revealed that MAD2 colocalized with RNF213 R4810K. Immunoprecipitation revealed an RNF213/MAD2 complex: R4810K formed a complex with MAD2 more readily than RNF213 wild-type. Desynchronized localization of MAD2 was observed more frequently during mitosis in fibroblasts from patients (n = 3, 61.0 ± 8.2%) compared with wild-type subjects (n = 6, 13.1 ± 7.7%; p < 0.01). Aneuploidy was observed more frequently in fibroblasts (p < 0.01) and induced pluripotent stem cells (iPSCs) (p < 0.03) from patients than from wild-type subjects. Vascular endothelial cells differentiated from iPSCs (iPSECs) of patients and an unaffected carrier had a longer time from prometaphase to metaphase than those from controls (p < 0.05). iPSECs from the patients and unaffected carrier had significantly increased mitotic failure rates compared with controls (p < 0.05). Thus, RNF213 R4810K induced mitotic abnormalities and increased risk of genomic instability

  11. Temporal profile of magnetic resonance angiography and decreased ratio of regulatory T cells after immunological adjuvant administration to mice lacking RNF213, a susceptibility gene for moyamoya disease.

    Kanoke, Atsushi; Fujimura, Miki; Niizuma, Kuniyasu; Fujimura, Taku; Kakizaki, Aya; Ito, Akira; Sakata, Hiroyuki; Sato-Maeda, Mika; Kure, Shigeo; Tominaga, Teiji

    2016-07-01

    Moyamoya disease (MMD) is a chronic, occlusive cerebrovascular disease with an unknown etiology and is characterized by an abnormal vascular network at the base of the brain. Recent studies identified the RNF213 gene (RNF213) as an important susceptibility gene for MMD; however, the mechanisms underlying the RNF213 abnormality related to MMD have not yet been elucidated. We previously reported that Rnf213-deficient mice and Rnf213 p. R4828K knock-in mice did not spontaneously develop MMD, indicating the importance of secondary insults in addition to genetic factors in the pathogenesis of MMD. The most influential secondary insult is considered to be an immunological reaction because RNF213 is predominantly expressed in immunological tissues. Therefore, we herein attempted to evaluate the role of an immunological stimulation as a supplementary insult to the target disruption of RNF213 in the pathophysiology of MMD. Rnf213-deficient mice were treated with strong immunological adjuvants including muramyl dipeptide (MDP)-Lys (L18), and then underwent time-sequential magnetic resonance angiography (MRA) up to 40 weeks of age. The results obtained did not reveal any characteristic finding of MMD, and no significant difference was observed in MRA findings or the anatomy of the circle of Willis between Rnf213-deficient mice and wild-type mice after the administration of MDP-Lys (L18). The ratio of regulatory T cells after the administration of MDP-Lys (L18) was significantly decreased in Rnf213-deficient mice (p<0.01), suggesting the potential role of the RNF213 abnormality in the differentiation of regulatory T cells. Although the mechanisms underlying the development of MMD currently remain unclear, the RNF213 abnormality may compromise immunological self-tolerance, thereby contributing to the development of MMD. PMID:26972532

  12. Fission dynamics of the compound nucleus 213Fr formed in heavy-ion-induced reactions

    Hadi Eslamizadeh

    2013-04-01

    A stochastic approach based on one-dimensional Langevin equations was used to calculate the average pre-fission multiplicities of neutrons, light charged particles and the fission probabilities for the compound nucleus 213Fr and the results are compared with the experimental data. In these calculations, a modified wall and window dissipation with a reduction coefficient, $k_{s}$ , has been used in the Langevin equations. It was shown that the results of the calculations are in good agreement with the experimental data by using values of $k_{s}$ in the range $0.3 ≤ k_{s} ≤ 0.5.$

  13. On the Use of an ER-213 Detonator to Establish a Baseline for the ER-486

    Thomas, Keith A. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Liechty, Gary H. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Jaramillo, Dennis C. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Munger, Alan C. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); McHugh, Douglas C. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Kennedy, James E. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2014-08-19

    This report documents a series of tests using a TSD-115 fireset coupled with an ER-213, a gold exploding bridgewire (EBW) detonator. These tests were designed to fire this EBW with a smaller fireset to obtain current and voltage data as well as timing information at voltage levels below, above, and throughout the threshold firing region. This study could then create a database for comparison to our current ER-486 EBW development, which is designed to be a lower voltage (<500V) device.

  14. Spectroscopy and octupole coupling of high-spin states in 213Rn

    Excited states of 213Rn, up to spins of ∼ 55/2 ℎ and an excitation energy of ∼ 6 MeV, have been studied using γ-ray and electron spectroscopy following the reactions 208Pb(9Be,4n) and 204Hg(13C,4n). Eight isomeric states were identified and g-factors for five of these measured by the TDPAD technique. Several of the isomeric states decay by enhanced E3 transitions. The level scheme and electromagnetic properties of the isomers are compared with the results of semi-empirical shell-model calculations including calculations which explicitly account for the particle-octupole vibration coupling

  15. Neutron response matrix for unfolding NE-213 measurements to 21 MeV

    A neutron response matrix from measured neutron responses of NE-213 in the energy range of 0.2 to 22 MeV is presented. An interpolation scheme was used to construct an 81-column matrix from the data of Verbinski, Burrus, Love, Zobel, and Hill. As a test of the new response matrix, the Cf-252 neutron spectrum was measured and unfolded using the new response matrix and the FORIST unfolding code. The spectrum agrees well with previous measurements at lower energies, while providing new information above 8 MeV

  16. On the Effect of Fluorination of 2,1,3-Benzothiadiazole

    Nielsen, Christian B.

    2015-04-22

    The 4,7-dithieno-2,1,3-benzothiadiazole (DTBT) moiety and its fluorinated counterpart are important pi-conjugated building blocks in the field of organic electronics. Here we present a combined experimental and theoretical investigation into fundamental properties relating to these two molecular entities and discuss the potential impact on extended π-conjugated materials and their electronic properties. While the fluorinated derivative, in the solid state, packs with a smaller co-facial overlap than DTBT, we report experimental evidence for stronger optical absorption as well as stronger intra- and intermolecular contacts upon fluorination.

  17. A Case with 46,XX,dup(X(q21.3q24 karyotype

    Selda Şimşek

    2010-03-01

    Full Text Available The relationship between phenotype and Xq duplicationsin females remains unclear. Some females are normal;some have short stature; and others have features suchas microcephaly, developmental delay/mental retardation,body asymmetries, and gonadal dysgenesis. Somefeatures in these females resemble those in Turner syndrome.We, herein, presented a 15 years-old girl withshort stature and primary amenorrhea, who was referredto cytogenetic laboratory. Through karyotipe analysis performedby Giemsa banding technique, the patient wasdetermined to have positive Barr body and 46,XX,dup(X(q21.3q24 chromosomal constitution. Case was discussedaccording to information of present literatures.

  18. EVN Observations of HESS J1943+213: Evidence for an Extreme TeV BL Lac Object

    Akiyama, Kazunori; Tanaka, Yasuyuki T; Nagai, Hiroshi; Giroletti, Marcello; Honma, Mareki

    2016-01-01

    We report on the 1.6 GHz (18 cm) VLBI observations of the unresolved, steady TeV source HESS J1943+213 located in the Galactic plane, performed with the European VLBI Network (EVN) in 2014. Our new observations with a nearly full EVN array provide the deepest image of HESS J1943+213 at the highest resolution ever achieved, enabling us to resolve the long-standing issues of the source identification. The milliarcsecond-scale structure of HESS J1943+213 has a clear asymmetric morphology, consisting of a compact core and a diffuse jet-like tail. This is broadly consistent with the previous e-EVN observations of the source performed in 2011, and re-analyzed in this work. The core component is characterized by the brightness temperature of $\\gtrsim1.8 \\times 10^9$ K, which is typical for low-luminosity blazars in general. Overall, radio properties of HESS J1943+213 are consistent with the source classification as an "extreme high-frequency-peaked BL Lac object". Remarkably, we note that since HESS J1943+213 does n...

  19. Split Hand/Foot Malformation Associated with 7q21.3 Microdeletion: A Case Report.

    Sivasankaran, Aswini; Srikanth, Ambika; Kulshreshtha, Pooja S; Anuradha, Deenadayalu; Kadandale, Jayarama S; Samuel, Chandra R

    2016-02-01

    Split hand/foot malformation (SHFM) or ectrodactyly is a rare genetic condition affecting limb development. SHFM shows clinical and genetic heterogeneity. It can present as an isolated form or in combination with additional anomalies affecting the long bones (nonsyndromic form) or other organ systems including the craniofacial, genitourinary and ectodermal structures (syndromic ectrodactyly). This study reports a girl with SHFM who also exhibited developmental delay, mild dysmorphic facial features and sensorineural hearing loss. High-resolution banding analysis indicated an interstitial deletion within the 7q21 band. FISH using locus-specific BAC probes confirmed the microdeletion of 7q21.3. Chromosomal microarray analysis also revealed a microdeletion of 1.856 Mb in 7q21.3. However, a larger 8.44-Mb deletion involving bands 7q21.11q21.2 was observed, and the breakpoints were refined. The phenotype and the candidate genes underlying the pathogenesis of this disorder are discussed. PMID:27022330

  20. Chains of dense cores in the Taurus L1495/B213 complex

    Tafalla, M

    2014-01-01

    (Abridged) We study the kinematics of the dense gas in the Taurus L1495/B213 filamentary region to investigate the mechanism of core formation. We use observations of N2H+(1-0) and C18O(2-1) carried out with the IRAM 30m telescope. We find that the dense cores in L1495/B213 are significantly clustered in linear chain-like groups about 0.5pc long. The internal motions in these chains are mostly subsonic and the velocity is continuous, indicating that turbulence dissipation in the cloud has occurred at the scale of the chains and not at the smaller scale of the individual cores. The chains also present an approximately constant abundance of N2H+ and radial intensity profiles that can be modeled with a density law that follows a softened power law. A simple analysis of the spacing between the cores using an isothermal cylinder model indicates that the cores have likely formed by gravitational fragmentation of velocity-coherent filaments. Combining our analysis of the cores with our previous study of the large-sc...

  1. HESS J1943+213: a candidate extreme BL Lacertae object

    Abramowski, A; Aharonian, F; Akhperjanian, A G; Anton, G; Balzer, A; Barnacka, A; de Almeida, U Barres; Bazer-Bachi, A R; Becherini, Y; Becker, J; Behera, B; Bernlöhr, K; Bochow, A; Boisson, C; Bolmont, J; Bordas, P; Borrel, V; Brucker, J; Brun, F; Brun, P; Bulik, T; Büsching, I; Carrigan, S; Casanova, S; Cerruti, M; Chadwick, P M; Charbonnier, A; Chaves, R C G; Cheesebrough, A; Chounet, L -M; Clapson, A C; Coignet, G; Colom, P; Conrad, J; Dalton, M; Daniel, M K; Davids, I D; Degrange, B; Deil, C; Dickinson, H J; Djannati-Ataï, A; Domainko, W; Drury, L O'C; Dubois, F; Dubus, G; Dyks, J; Dyrda, M; Egberts, K; Eger, P; Espigat, P; Fallon, L; Farnier, C; Fegan, S; Feinstein, F; Fernandes, M V; Fiasson, A; Fontaine, G; Förster, A; Fü\\ssling, M; Gallant, Y A; Gast, H; Gérard, L; Gerbig, D; Giebels, B; Glicenstein, J F; Glück, B; Goret, P; Göring, D; Häffner, S; Hague, J D; Hampf, D; Hauser, M; Heinz, S; Heinzelmann, G; Henri, G; Hermann, G; Hinton, J A; Hoffmann, A; Hofmann, W; Hofverberg, P; Holler, M; Horns, D; Jacholkowska, A; de Jager, O C; Jahn, C; Jamrozy, M; Jung, I; Kastendieck, M A; Katarzyński, K; Katz, U; Kaufmann, S; Keogh, D; Khangulyan, D; Khélifi, B; Klochkov, D; Kluźniak, W; Kneiske, T; Komin, Nu; Kosack, K; Kossakowski, R; Laffon, H; Lamanna, G; Lennarz, D; Lohse, T; Lopatin, A; Lu, C -C; Marandon, V; Marcowith, A; Masbou, J; Maurin, D; Maxted, N; McComb, T J L; Medina, M C; Méhault, J; Nguyen, N; Moderski, R; Moulin, E; Naumann, C L; Naumann-Godo, M; de Naurois, M; Nedbal, D; Nekrassov, D; Nicholas, B; Niemiec, J; Nolan, S J; Ohm, S; Olive, J-F; Wilhelmi, E de Oña; Opitz, B; Ostrowski, M; Panter, M; Arribas, M Paz; Pedaletti, G; Pelletier, G; Petrucci, P -O; Pita, S; Pühlhofer, G; Punch, M; Quirrenbach, A; Raue, M; Rayner, S M; Reimer, A; Reimer, O; Renaud, M; Reyes, R de los; Rieger, F; Ripken, J; Rob, L; Rosier-Lees, S; Rowell, G; Rudak, B; Rulten, C B; Ruppel, J; Ryde, F; Sahakian, V; Santangelo, A; Schlickeiser, R; Schöck, F M; Schönwald, A; Schulz, A; Schwanke, U; Schwarzburg, S; Schwemmer, S; Shalchi, A; Sikora, M; Skilton, J L; Sol, H; Spengler, G; Stawarz, \\L; Steenkamp, R; Stegmann, C; Stinzing, F; Stycz, K; Sushch, I; Szostek, A; Tavernet, J -P; Terrier, R; Tibolla, O; Tluczykont, M; Valerius, K; van Eldik, C; Vasileiadis, G; Venter, C; Vialle, J P; Viana, A; Vincent, P; Vivier, M; Völk, H J; Volpe, F; Vorobiov, S; Vorster, M; Wagner, S J; Ward, M; Wierzcholska, A; Zajczyk, A; Zdziarski, A A; Zech, A; Zechlin, H -S; Burnett, T H; Hill, A B

    2011-01-01

    We report on a newly detected point-like source, HESS J1943+213 located in the Galactic plane. This source coincides with an unidentified hard X-ray source IGR J19443+2117, which was proposed to have radio and infrared counterparts. HESS J1943+213 is detected at the significance level of 7.9 \\sigma (post-trials) at RA(J2000)=19h 43m 55s +- 1s (stat) +- 1s (sys), DEC(J2000) = +21deg 18' 8" +- 17" (stat) +- 20" (sys). The source has a soft spectrum with photon index Gamma = 3.1 +- 0.3 (stat) +- 0.2 (sys) and a flux above 470 GeV of 1.3 +- 0.2 (stat) +- 0.3 (sys) x 10^{-12} cm^{-2} s^{-1}. There is no Fermi/LAT counterpart down to a flux limit of 6 x 10^{-9} cm^{-2} s^{-1} in the 0.1-100 GeV energy range (95% confidence upper limit calculated for an assumed power-law model with a photon index Gamma=2.0). The data from radio to VHE gamma-rays do not show any significant variability. We combine new H.E.S.S., Fermi/LAT and Nancay Radio Telescope observations with pre-existing non-simultaneous multi-wavelength obser...

  2. Benchmark calculation with improved VVER-440/213 RPV CFD model

    A detailed RPV model of WWER-440/213 type reactors was developed in BME NTI in the last years. This model contains the main structural elements as inlet and outlet nozzles, guide baffles of hydro-accumulators, alignment drifts, perforated plates, brake- and guide tube chamber and simplified core. For the meshing and simulations ANSYS software's (ICEM 12.0 and CFX 12.0) were used. With the new vessel model a series of parameter studies were performed considering turbulence models, discretization schemes, and modeling methods. In steady state the main results were presented on last AER Symposium in Varna. The model is suitable for different transient calculations as well. The purpose of the suggested new benchmark (seventh Dynamic AER Benchmark) is to investigate the reactor dynamic effects of coolant mixing in the WWER-440/213 reactor vessel and to compare the different codes. The task of this benchmark is to investigate the start up of the sixth main coolant pump. The computation was carried out with the help of ATHLET/BIPRVVER code in Kurchatov Institute for this transient and was repeated with ANSYS CFX 12.0 at our Institute. (Authors)

  3. An investigation on SA 213-Tube to SA 387-Tube plate using friction welding process

    Friction welding of tube to tube plate using an external tool (FWTPET) is a relatively newer solid state welding process used for joining tube to tube plate of either similar or dissimilar materials with enhanced mechanical and metallurgical properties. In the present study, FWTPET has been used to weld SA 213 (Grade T12) tube with SA 387 (Grade 22) tube plate. The welded samples are found to have satisfactory joint strength and the Energy dispersive X-ray analysis (EDX), X-ray diffraction (XRD) study showed that inter metallic compound is absent in the weld zone. The different weld joints have been identified and the phase composition is found using EDX and XRD. Microstructures have been analyzed using optical and Scanning electron microscopy (SEM). The mechanical properties such as hardness, compressive shear strength and peel test for different weld conditions are studied and the hardness survey revealed that there is increase in hardness at the weld interface due to grain refinement. The corrosion behavior for different weld conditions have been analyzed and the weld zone is found to have better corrosion resistance due to the influence of the grain refinement after FWTPET welding process. Hence, the present investigation is carried out to study the behavior of friction welded dissimilar joints of SA 213 tube and SA 387 tube plate joints and the results are presented. The present study confirms that a high quality tube to tube plate joint can be achieved using FWTPET process at 1120 rpm

  4. Radioimmunotherapy Using Vascular Targeted 213Bi: The Role of TNF-Alpha in the Development of Pulmonary Fibrosis

    Davis, I.A.; Kennel, S.J.

    1998-10-14

    A monoclonal antibody (201B) specific to murine thrombomodulin, covalently linked to CHX-b-DTPA, successfully delivers chelated 213Bi, an {alpha}-particle emitter, (213Bi-201B) rapidly to lungvascular endothelium. When injected at doses of l MBq/mouse, 213Bi-201B destroyed most of the 100 colonies of EMT-6 mammary carcinomas growing as lung tumors of up to 2000 cells/colony. Some mice were cured of lung tumors and others had extended life-spans compared to untreated control animals but eventually succumbed to tumor recurrence. At injected doses of 4-6 MBq/mouse, 100% of lung tumor colonies were eliminated; however, 3-4 months later these mice developed pulmonary fibrosis and died. The mechanisms leading to the fibrotic response in other pulmonary irradiation models strongly implicate tumor necrosis factor-alpha (TNF-{alpha}), released from damaged tissues, as the pivotal inflammatory cytokine in a cascade of events which culminate in fibrosis. Attempts to prevent the development of pulmonary fibrosis, by using antibodies or soluble receptor (Enbrel{trademark}) as inhibitors of TNF-{alpha}, were unsuccessful. Additionally, mice genetically deficient for TNF-{alpha} production developed pulmonary fibrosis following 213Bi-201B treatment. Interestingly, non-tumor bearing BALB/c mice receiving Enbrel{trademark} or mice genetically deficient in TNF-{alpha} production and treated with 213Bi-201B, had significantly reduced life spans compared to mice receiving no treatment or 213Bi-201B alone. We speculate that, in normal mice, while TNF-{alpha} may induce an inflammatory response following {alpha}-particle radiation mediated tumor clearance and pulmonary damage, its effects in the post-tumor clearance time period may actually retard the development of fibrosis.

  5. Antibacterial activity and genotypic-phenotypic characteristics of bacteriocin-producing Bacillus subtilis KKU213: potential as a probiotic strain.

    Khochamit, Nalisa; Siripornadulsil, Surasak; Sukon, Peerapol; Siripornadulsil, Wilailak

    2015-01-01

    The antimicrobial activity and probiotic properties of Bacillus subtilis strain KKU213, isolated from local soil, were investigated. The cell-free supernatant (CFS) of a KKU213 culture containing crude bacteriocins exhibited inhibitory effects on Gram-positive bacteria, including Bacillus cereus, Listeria monocytogenes, Micrococcus luteus, and Staphylococcus aureus. The antibacterial activity of the CFS precipitated with 40% ammonium sulfate (AS) remained even after treatment at 60 and 100 °C, at pH 4 and 10 and with proteolytic enzymes, detergents and heavy metals. When analyzed by SDS-PAGE and overlaid with the indicator strains B. cereus and S. aureus, the 40% AS precipitate exhibited inhibitory activity on proteins smaller than 10 kDa. However, proteins larger than 25 kDa and smaller than 10 kDa were still observed on a native protein gel. Purified subtilosin A was prepared by Amberlite XAD-16 bead extraction and HPLC and analyzed by Nano-LC-QTOF-MS. Its molecular mass was found to be 3.4 kDa, and it retained its antibacterial activity. These results are consistent with the detection of the anti-listerial subtilosin A gene of the sbo/alb cluster in the KKU213 strain, which is 100% identical to that of B. subtilis subsp. subtilis 168. In addition to stable and cyclic subtilosin A, a mixture of many extracellular antibacterial peptides was also detected in the KKU213 culture. The KKU213 strain produced extracellular amylase, cellulase, lipase and protease, is highly acid-resistant (pH 2) when cultured in inulin and promotes health and reduces infection of intestinally colonized broiler chickens. Therefore, we propose that bacteriocin-producing B. subtilis KKU213 could be used as a potential probiotic strain or protective culture. PMID:25440998

  6. 17DD and 17D-213/77 yellow fever substrains trigger a balanced cytokine profile in primary vaccinated children.

    Ana Carolina Campi-Azevedo

    Full Text Available BACKGROUND: This study aimed to compare the cytokine-mediated immune response in children submitted to primary vaccination with the YF-17D-213/77 or YF-17DD yellow fever (YF substrains. METHODS: A non-probabilistic sample of eighty healthy primary vaccinated (PV children was selected on the basis of their previously known humoral immune response to the YF vaccines. The selected children were categorized according to their YF-neutralizing antibody titers (PRNT and referred to as seroconverters (PV-PRNT(+ or nonseroconverters (PV-PRNT(-. Following revaccination with the YF-17DD, the PV-PRNT(- children (YF-17D-213/77 and YF-17DD groups seroconverted and were referred as RV-PRNT(+. The cytokine-mediated immune response was investigated after short-term in vitro cultures of whole blood samples. The results are expressed as frequency of high cytokine producers, taking the global median of the cytokine index (YF-Ag/control as the cut-off. RESULTS: The YF-17D-213/77 and the YF-17DD substrains triggered a balanced overall inflammatory/regulatory cytokine pattern in PV-PRNT(+, with a slight predominance of IL-12 in YF-17DD vaccinees and a modest prevalence of IL-10 in YF-17D-213/77. Prominent frequency of neutrophil-derived TNF-α and neutrophils and monocyte-producing IL-12 were the major features of PV-PRNT(+ in the YF-17DD, whereas relevant inflammatory response, mediated by IL-12(+CD8(+ T cells, was the hallmark of the YF-17D-213/77 vaccinees. Both substrains were able to elicit particular but relevant inflammatory events, regardless of the anti-YF PRNT antibody levels. PV-PRNT(- children belonging to the YF-17DD arm presented gaps in the inflammatory cytokine signature, especially in terms of the innate immunity, whereas in the YF-17D-213/77 arm the most relevant gap was the deficiency of IL-12-producing CD8(+T cells. Revaccination with YF-17DD prompted a balanced cytokine profile in YF-17DD nonresponders and a robust inflammatory profile in YF-17D

  7. 17DD and 17D-213/77 Yellow Fever Substrains Trigger a Balanced Cytokine Profile in Primary Vaccinated Children

    Luiza-Silva, Maria; Batista, Maurício Azevedo; Martins, Marina Angela; Sathler-Avelar, Renato; da Silveira-Lemos, Denise; Camacho, Luiz Antonio Bastos; de Menezes Martins, Reinaldo; de Lourdes de Sousa Maia, Maria; Farias, Roberto Henrique Guedes; da Silva Freire, Marcos; Galler, Ricardo; Homma, Akira; Ribeiro, José Geraldo Leite; Lemos, Jandira Aparecida Campos; Auxiliadora-Martins, Maria; Caldas, Iramaya Rodrigues; Elói-Santos, Silvana Maria; Teixeira-Carvalho, Andréa; Martins-Filho, Olindo Assis

    2012-01-01

    Background This study aimed to compare the cytokine-mediated immune response in children submitted to primary vaccination with the YF-17D-213/77 or YF-17DD yellow fever (YF) substrains. Methods A non-probabilistic sample of eighty healthy primary vaccinated (PV) children was selected on the basis of their previously known humoral immune response to the YF vaccines. The selected children were categorized according to their YF-neutralizing antibody titers (PRNT) and referred to as seroconverters (PV-PRNT+) or nonseroconverters (PV-PRNT−). Following revaccination with the YF-17DD, the PV-PRNT− children (YF-17D-213/77 and YF-17DD groups) seroconverted and were referred as RV-PRNT+. The cytokine-mediated immune response was investigated after short-term in vitro cultures of whole blood samples. The results are expressed as frequency of high cytokine producers, taking the global median of the cytokine index (YF-Ag/control) as the cut-off. Results The YF-17D-213/77 and the YF-17DD substrains triggered a balanced overall inflammatory/regulatory cytokine pattern in PV-PRNT+, with a slight predominance of IL-12 in YF-17DD vaccinees and a modest prevalence of IL-10 in YF-17D-213/77. Prominent frequency of neutrophil-derived TNF-α and neutrophils and monocyte-producing IL-12 were the major features of PV-PRNT+ in the YF-17DD, whereas relevant inflammatory response, mediated by IL-12+CD8+ T cells, was the hallmark of the YF-17D-213/77 vaccinees. Both substrains were able to elicit particular but relevant inflammatory events, regardless of the anti-YF PRNT antibody levels. PV-PRNT− children belonging to the YF-17DD arm presented gaps in the inflammatory cytokine signature, especially in terms of the innate immunity, whereas in the YF-17D-213/77 arm the most relevant gap was the deficiency of IL-12-producing CD8+T cells. Revaccination with YF-17DD prompted a balanced cytokine profile in YF-17DD nonresponders and a robust inflammatory profile in YF-17D-213/77 nonresponders

  8. Safety improvement and results of commissioning of Mochovce NPP WWER 440/213

    Mochovce NPP is the last one of this kind and compared to its predecessors, it is characterized by several modifications which contribute to the improvement of the safety level. In addition based on Nuclear Regulatory Authority requirements and based on documents: - IAEA - Safety Issues and their ranking for NPP WWER 440/213, - IAEA - Safety Improvement of Mochovce NPP Project Review Mission, - Riskaudit - Evaluation of the Mochovce NPP Safety Improvements. Additional safety measures have been implemented before commissioning. The consortium EUCOM (FRAMATOME - SIEMENS), SKODA Praha, ENERGOPROJEKT Praha, Russian organizations and VUJE Trnava Nuclear Power Plants research institute were selected for design and implementation of the safety measures. The papers summarized, safety requirements, safety measures implemented, results of commissioning and results of safety analysis report evaluation. (author)

  9. Seismic margin assessment and earthquake experience based methods for WWER-440/213 type NPPs

    This report covers the review of the already completed studies, namely, safe shutdown system identification and classification for Bohunice NPP and the comparative study of standards and criteria. It contains a report on currently ongoing studies concerning seismic margin assessment and earthquake experience based methods in application for seismic evaluation and verification of structures and equipment components of the operating WWER-440/213 type NPPs. This is based on experiences obtained from Paks NPP. The work plan for the remaining period of Benchmark CRP and the new proposals are included. These are concerned with seismic evaluation of selected safety related mechanical equipment and pipes of Paks NPP, and the actual seismic issues of the Temelin WWER-1000 type NPP

  10. Neuropathic pain in patients with spinal cord injury: report of 213 patients

    Manoel Jacobsen Teixeira

    2013-09-01

    Full Text Available Objective Management of neuropathic pain following spinal cord injury (SCI can be a frustrating experience for patients since it poses a therapeutic challenge. In this article the authors describe the clinical characteristics of a group of patients with pain after spinal cord injury. Methods In this retrospective study, 213 patients with SCI and neuropathic pain were assessed. We analyzed clinical characteristics, treatment options, and pain intensity for these patients. Results The main cause of SCI was spine trauma, which occurred in 169 patients, followed by tumors and infection. Complete lesions were verified in 144 patients. In our study, patients with traumatic SCI and partial lesions seem to be presented with more intense pain; however, this was not statistically significant. Conclusions Neuropathic pain is a common complaint in patients with SCI and presents a treatment challenge. Knowledge of the clinical characteristics of this group of patients may help determine the best approach to intervention.

  11. The effect of low temperature on the scintillation efficiency of liquid scintillator NE 213

    Ul-Haq, F.; Butt, M.Z.; Ali, W.; Jamil, S.; Durrani, S.A. (Government Coll., Lahore (Pakistan). Nuclear Research Lab.)

    1990-01-01

    The scintillation response of the liquid scintillator NE 213 has been studied as function of temperature in the range 225 to 300 K. It has been found that the light output under gamma excitation increases with decrease in temperature. The data are well represented by the relation: I{sub low} - I = I{sub 0} exp(- E/kT), where I is the count rate at temperature T, I{sub low} is a constant equal to 750 counts/minute, the pre-exponential factor I{sub 0} is 15 x 10{sup 5} counts/minute, k is the Boltzmann constant and E is the activation energy equal to 0.21 eV, which is typical for a diffusion controlled process in the temperature range studied. (author).

  12. Light Curves of 213 Type Ia Supernovae from the ESSENCE Survey

    Narayan, Gautham; Tucker, Brad E; Foley, Ryan J; Wood-Vasey, W Michael; Challis, Peter; Stubbs, Christopher W; Kirshner, Robert P; Aguilera, Claudio; Becker, Andrew C; Blondin, Stephane; Clocchiatti, Alejandro; Covarrubias, Ricardo; Damke, Guillermo; Davis, Tamara M; Filippenko, Alexei V; Ganeshalingam, Mohan; Garg, Arti; Garnavich, Peter M; Hicken, Malcolm; Jha, Saurabh W; Krisciunas, Kevin; Leibundgut, Bruno; Li, Weidong; Matheson, Thomas; Miknaitis, Gajus; Pignata, Guiliano; Prieto, Jose Luis; Riess, Adam G; Schmidt, Brian P; Silverman, Jeffrey M; Smith, R Chris; Sollerman, Jesper; Spyromilio, Jason; Suntzeff, Nicholas B; Tonry, John L; Zenteno, Alfredo

    2016-01-01

    The ESSENCE survey discovered 213 Type Ia supernovae at redshifts 0.1 < z < 0.81 between 2002 and 2008. We present their R and I-band photometry, measured from images obtained using the MOSAIC II camera at the CTIO 4 m Blanco telescope, along with rapid-response spectroscopy for each object. We use our spectroscopic follow-up observations to determine an accurate, quantitative classification and a precise redshift. Through an extensive calibration program we have improved the precision of the CTIO Blanco natural photometric system. We use several empirical metrics to measure our internal photometric consistency and our absolute calibration of the survey. We assess the effect of various potential sources of systematic bias on our measured fluxes, and we estimate that the dominant term in the systematic error budget from the photometric calibration on our absolute fluxes is ~1%.

  13. Synthesis of New 2-(1,3-Dithianylphenols and Hexakis-[p-(1,3-dithian-2-ylphenoxy]cyclotriphosphazene

    Prónayová Nadezda

    1997-01-01

    Full Text Available 2-Chloro-1,3-dithiane was obtained by the chlorination of 1,3-dithiane with N-chlorosuccinimide. Reactions of 2-chloro-1,3-dithiane with various substituted phenols lead to 2-(1,3-dithianylphenols (3. Hexakis-[p-(1,3-dithian-2-ylphenoxy]cyclotriphosphazene (6 was obtained by reaction with hexachlorotriazacyclotriphosphazene (5.

  14. Identification of RNF213 as a susceptibility gene for moyamoya disease and its possible role in vascular development.

    Wanyang Liu

    Full Text Available BACKGROUND: Moyamoya disease is an idiopathic vascular disorder of intracranial arteries. Its susceptibility locus has been mapped to 17q25.3 in Japanese families, but the susceptibility gene is unknown. METHODOLOGY/PRINCIPAL FINDINGS: Genome-wide linkage analysis in eight three-generation families with moyamoya disease revealed linkage to 17q25.3 (P<10(-4. Fine mapping demonstrated a 1.5-Mb disease locus bounded by D17S1806 and rs2280147. We conducted exome analysis of the eight index cases in these families, with results filtered through Ng criteria. There was a variant of p.N321S in PCMTD1 and p.R4810K in RNF213 in the 1.5-Mb locus of the eight index cases. The p.N321S variant in PCMTD1 could not be confirmed by the Sanger method. Sequencing RNF213 in 42 index cases confirmed p.R4810K and revealed it to be the only unregistered variant. Genotyping 39 SNPs around RNF213 revealed a founder haplotype transmitted in 42 families. Sequencing the 260-kb region covering the founder haplotype in one index case did not show any coding variants except p.R4810K. A case-control study demonstrated strong association of p.R4810K with moyamoya disease in East Asian populations (251 cases and 707 controls with an odds ratio of 111.8 (P = 10(-119. Sequencing of RNF213 in East Asian cases revealed additional novel variants: p.D4863N, p.E4950D, p.A5021V, p.D5160E, and p.E5176G. Among Caucasian cases, variants p.N3962D, p.D4013N, p.R4062Q and p.P4608S were identified. RNF213 encodes a 591-kDa cytosolic protein that possesses two functional domains: a Walker motif and a RING finger domain. These exhibit ATPase and ubiquitin ligase activities. Although the mutant alleles (p.R4810K or p.D4013N in the RING domain did not affect transcription levels or ubiquitination activity, knockdown of RNF213 in zebrafish caused irregular wall formation in trunk arteries and abnormal sprouting vessels. CONCLUSIONS/SIGNIFICANCE: We provide evidence suggesting, for the first

  15. Double-pulse laser ablation sampling: Enhancement of analyte emission by a second laser pulse at 213 nm

    For the purpose of devising methods for minimally destructive multi-element analysis, we compare the performance of a 266 nm–213 nm double-pulse scheme against that of the single 266 nm pulse scheme. The first laser pulse at 266 nm ablates a mica sample. Ten ns later, the second pulse at 213 nm and 64 mJ cm−2 orthogonally intercepts the gas plume to enhance the analyte signal. Emissions from aluminum, silicon, magnesium and sodium are simultaneously observed. At low 266 nm laser fluence when only sub-ng of sample mass is removed, the signal enhancement by the 213 nm pulse is especially apparent. The minimum detectable amount of aluminum is about 24 fmol; it will be a hundred times higher if the sample is analyzed by the 266 nm pulse alone. The minimum detectable mass for the other analytes is also reduced by about two orders of magnitude when the second pulse at 213 nm is introduced. The spectral and temporal properties of the enhanced signal are consistent with the mechanism of ultra-violet laser excited atomic fluorescence of dense plumes. - Highlights: • We devise a two-laser-pulse scheme to analyze the elemental composition of mica as test samples. • We compare the analytical performance of the single 266 nm pulse scheme against the 266 nm – 213 nm two pulse scheme. • The two pulse scheme improves the absolute LODs of the analytes by about a hundred times. • The spectral and temporal properties of the enhanced signal are consistent with the mechanism

  16. Double-pulse laser ablation sampling: Enhancement of analyte emission by a second laser pulse at 213 nm

    Cai, Bruno Yue [Laser Technologies Group, Lawrence Berkeley National Laboratory, Berkeley, CA (United States); Department of Physics, Hong Kong Baptist University, Kowloon Tong, Hong Kong (China); Mao, Xianglei [Laser Technologies Group, Lawrence Berkeley National Laboratory, Berkeley, CA (United States); Hou, Huaming [Laser Technologies Group, Lawrence Berkeley National Laboratory, Berkeley, CA (United States); Ocean University of China, Qingdao (China); Zorba, Vassilia; Russo, Richard E. [Laser Technologies Group, Lawrence Berkeley National Laboratory, Berkeley, CA (United States); Cheung, Nai-Ho, E-mail: nhcheung@hkbu.edu.hk [Department of Physics, Hong Kong Baptist University, Kowloon Tong, Hong Kong (China)

    2015-08-01

    For the purpose of devising methods for minimally destructive multi-element analysis, we compare the performance of a 266 nm–213 nm double-pulse scheme against that of the single 266 nm pulse scheme. The first laser pulse at 266 nm ablates a mica sample. Ten ns later, the second pulse at 213 nm and 64 mJ cm{sup −2} orthogonally intercepts the gas plume to enhance the analyte signal. Emissions from aluminum, silicon, magnesium and sodium are simultaneously observed. At low 266 nm laser fluence when only sub-ng of sample mass is removed, the signal enhancement by the 213 nm pulse is especially apparent. The minimum detectable amount of aluminum is about 24 fmol; it will be a hundred times higher if the sample is analyzed by the 266 nm pulse alone. The minimum detectable mass for the other analytes is also reduced by about two orders of magnitude when the second pulse at 213 nm is introduced. The spectral and temporal properties of the enhanced signal are consistent with the mechanism of ultra-violet laser excited atomic fluorescence of dense plumes. - Highlights: • We devise a two-laser-pulse scheme to analyze the elemental composition of mica as test samples. • We compare the analytical performance of the single 266 nm pulse scheme against the 266 nm – 213 nm two pulse scheme. • The two pulse scheme improves the absolute LODs of the analytes by about a hundred times. • The spectral and temporal properties of the enhanced signal are consistent with the mechanism.

  17. EVN Observations of HESS J1943+213: Evidence for an Extreme TeV BL Lac Object

    Akiyama, Kazunori; Stawarz, Łukasz; Tanaka, Yasuyuki T.; Nagai, Hiroshi; Giroletti, Marcello; Honma, Mareki

    2016-06-01

    We report on the 1.6 GHz (18 cm) very long baseline interferometry (VLBI) observations of the unresolved, steady TeV source HESS J1943+213 located in the Galactic plane, performed with the European VLBI Network (EVN) in 2014. Our new observations with a nearly full EVN array provide the deepest image of HESS J1943+213 at the highest resolution ever achieved, enabling us to resolve the long-standing issues of the source identification. The milliarcsecond-scale structure of HESS J1943+213 has a clear asymmetric morphology consisting of a compact core and a diffuse jet-like tail. This is broadly consistent with the previous e-EVN observations of the source performed in 2011 and re-analyzed in this work. The core component is characterized by the brightness temperature of ≳ 1.8× {10}9 K, which is typical for low-luminosity blazars in general. Overall, the radio properties of HESS J1943+213 are consistent with the source classification as an “extreme high-frequency-peaked BL Lac object.” Remarkably, we note that because HESS J1943+213 does not reveal any optical or infrared signatures of the active galactic nucleus activity, it would never be recognized and identified as a BL Lac object if not for its location close to the Galactic plane where the High Energy Stereoscopic System surveyed for and the follow-up dedicated X-ray and radio studies triggered by the source detection in the TeV range. Our results suggest, therefore, a presence of an unrecognized, possibly very numerous population of particularly extreme HBLs and simultaneously demonstrate that the low-frequency VLBI observations with high angular resolution are indispensable for a proper identification of such objects.

  18. Cell death triggered by alpha-emitting {sup 213}Bi-immunoconjugates in HSC45-M2 gastric cancer cells is different from apoptotic cell death

    Seidl, Christof; Schroeck, Hedwig; Seidenschwang, Sabine; Beck, Roswitha; Schwaiger, Markus; Senekowitsch-Schmidtke, Reingard [Technische Universitaet Muenchen, Department of Nuclear Medicine, Munich (Germany); Schmid, Ernst [National Research Center for Environment and Health, Institute of Radiation Biology, GSF, Neuherberg (Germany); Abend, Michael [German Armed Forces, Institute of Radiobiology, Munich (Germany); Becker, Karl-Friedrich [Technische Universitaet Muenchen, Institute of Pathology, Munich (Germany); National Research Center for Environment and Health, Institute of Pathology, GSF, Neuherberg (Germany); National Research Center for Environment and Health, Institute of Molecular Immunology, GSF, Munich (Germany); Apostolidis, Christos; Nikula, Tuomo K. [European Commission, Institute for Transuranium Elements, Karlsruhe (Germany); Kremmer, Elisabeth [National Research Center for Environment and Health, Institute of Molecular Immunology, GSF, Munich (Germany)

    2005-03-01

    Radioimmunotherapy with {alpha}-particle-emitting nuclides, such as{sup 213}Bi, is a promising concept for the elimination of small tumour nodules or single disseminated tumour cells. The aim of this study was to investigate cellular damage and the mode of cell death triggered by {sup 213}Bi-immunoconjugates. Human gastric cancer cells (HSC45-M2) expressing d9-E-cadherin were incubated with different levels of activity of {sup 213}Bi-d9MAb targeting d9-E-cadherin and {sup 213}Bi-d8MAb, which does not bind to d9-E-cadherin. Micronucleated (M) cells, abnormal (A) cells and apoptotic (A) [(MAA)] cells were scored microscopically in the MAA assay following fluorescent staining of nuclei and cytoplasm. Chromosomal aberrations were analysed microscopically following Giemsa staining. The effect of z-VAD-fmk, known to inhibit apoptosis, on the prevention of cell death was investigated following treatment of HSC45-M2 cells with sorbitol as well as {sup 213}Bi-d9MAb. Activation of caspase 3 after incubation of HSC45-M2 cells with both sorbitol and {sup 213}Bi-d9MAb was analysed via Western blotting. Following incubation of HSC45-M2 human gastric cancer cells expressing d9-E-cadherin with {sup 213}Bi-d9MAb the number of cells killed increased proportional to the applied activity concentration. Microscopically visible effects of {alpha}-irradiation of HSC45-M2 cells were formation of micronuclei and severe chromosomal aberrations. Preferential induction of these lesions with specific {sup 213}Bi-d9MAb compared with unspecific {sup 213}Bi-d8MAb (not targeting d9-E-cadherin) was not observed if the number of floating, i.e. unbound {sup 213}Bi-immunoconjugates per cell exceeded 2 x 10{sup 4}, most likely due to intense crossfire. In contrast to sorbitol-induced cell death, cell death triggered by {sup 213}Bi-immunoconjugates was independent of caspase 3 activation and could not be inhibited by z-VAD-fmk, known to suppress the apoptotic pathway. {sup 213}Bi-immunoconjugates seem

  19. Cell death triggered by alpha-emitting 213Bi-immunoconjugates in HSC45-M2 gastric cancer cells is different from apoptotic cell death

    Radioimmunotherapy with α-particle-emitting nuclides, such as213Bi, is a promising concept for the elimination of small tumour nodules or single disseminated tumour cells. The aim of this study was to investigate cellular damage and the mode of cell death triggered by 213Bi-immunoconjugates. Human gastric cancer cells (HSC45-M2) expressing d9-E-cadherin were incubated with different levels of activity of 213Bi-d9MAb targeting d9-E-cadherin and 213Bi-d8MAb, which does not bind to d9-E-cadherin. Micronucleated (M) cells, abnormal (A) cells and apoptotic (A) [(MAA)] cells were scored microscopically in the MAA assay following fluorescent staining of nuclei and cytoplasm. Chromosomal aberrations were analysed microscopically following Giemsa staining. The effect of z-VAD-fmk, known to inhibit apoptosis, on the prevention of cell death was investigated following treatment of HSC45-M2 cells with sorbitol as well as 213Bi-d9MAb. Activation of caspase 3 after incubation of HSC45-M2 cells with both sorbitol and 213Bi-d9MAb was analysed via Western blotting. Following incubation of HSC45-M2 human gastric cancer cells expressing d9-E-cadherin with 213Bi-d9MAb the number of cells killed increased proportional to the applied activity concentration. Microscopically visible effects of α-irradiation of HSC45-M2 cells were formation of micronuclei and severe chromosomal aberrations. Preferential induction of these lesions with specific 213Bi-d9MAb compared with unspecific 213Bi-d8MAb (not targeting d9-E-cadherin) was not observed if the number of floating, i.e. unbound 213Bi-immunoconjugates per cell exceeded 2 x 104, most likely due to intense crossfire. In contrast to sorbitol-induced cell death, cell death triggered by 213Bi-immunoconjugates was independent of caspase 3 activation and could not be inhibited by z-VAD-fmk, known to suppress the apoptotic pathway. 213Bi-immunoconjugates seem to induce a mode of cell death different from apoptosis in HSC45-M2 cells. (orig.)

  20. Experimental therapy of disseminated B-Cell lymphoma xenografts with 213Bi-labeled anti-CD74

    A 213Bi-labeled antibody to CD74 was evaluated as a therapeutic agent for B-cell lymphoma. The α-particle emission, with a half-life of 46 min, is appropriate for therapy of micrometastases. The labeled Ab retained full immunoreactivity, and was potent at single-cell kill of the Raji B-lymphoma cell line. Approximately 30 decays of cell-bound 213Bi was required for a cell kill of 99%, and dosimetry calculations suggested that the cGy dose delivered was sufficient to produce the level of toxicity observed. A non-reactive control Ab, labeled similarly, also produced toxicity, due to decays occurring in the medium, but was approximately 3-fold less potent than the reactive Ab. In a SCID mouse xenograft micrometastatic model, Ab injection at day 2 or day 5 after tumor inoculation resulted in strong, specific suppression of tumor growth, with some apparent cures

  1. High-performance liquid chromatographic determination of selenium in coal after derivatization to 2,1,3-benzoselenadiazoles

    Khuhawar, M.Y.; Bozdar, R.B.; Babar, M.A. (Sindh University, Jamshoro (Pakistan). Inst. of Chemistry)

    1992-11-01

    High-performance liquid chromatography was examined for the determination of selenium after derivatization to 2,1,3-benzoselenadiazoles using 1,2-diaminobenzene, 1,2-diamino-4-nitrobenzene (NDAB), 2,3-diaminonaphthalene and 3,3-diaminobenzidine as derivatizing agents. Elution was carried out using a mixture of chloroform and hexane, with ultraviolet spectrophotometric detection. The 2,1,3-benzoselenadiazoles were extracted into toluene. Linear calibrations were obtained for 0-4[mu]g of selenium in 10 cm[sup 3] of solution and the detection limits were 20-50 ng of selenium in 10 cm[sup 3] of solution. The method was applied to the determination of selenium in coal samples and a shampoo using NDAB as the derivatizing reagent in acidic solution.

  2. Completion of the VVER 440/213 NPP Mochovce incorporation enhanced safety features

    The cooperation between the western countries and the countries of ex-eastern block in the field of nuclear safety is recent and still limited. The main reasons for this situation are limited or non existent capabilities of these countries for financing as well as non acceptable legal conditions concerning the third party nuclear liability in this part of Europe. Nevertheless, Framatome and Siemens associated in the consortium named EUCOM, have signed in April 1996 the contract of about 100 million US dollars with Slovak electricity company (SLOVENSKE ELEKTRARNE-SE) for upgrading the Units 1 and 2 of Mochovce Nuclear Power Plant according to the western safety standards. This is the first important project involving west-european companies in the modernisation of Russian type of pressurized water reactor (VVER 440/213). The consortium will cooperate with other partners involved in the project: Slovak, Czech and Russian. The financing of the project will be provided mainly form Slovak and Czech sources. The safety upgrading will be financed through French and German buyer credits. French company Electricite de France (EDF) will be the consultant for SE. The safety upgrading measures have been elaborated taking into account the recommendation of Vienna International Atomic Energy Agency (IAEA) and the evaluation of the safety realised by RISKAUDIT, the common organization of German and French safety authorities (GSR and IPSN). Hence all guaranties have been taken to fulfil the western safety criteria for Nuclear Power Plant Mochovce. (author)

  3. Spin vectors in the Koronis family: comprehensive results from two independent analyses of 213 rotation lightcurves

    Slivan, Stephen M.; Binzel, Richard P.; Crespo da Silva, Lucy D.; Kaasalainen, Mikko; Lyndaker, Mariah M.; Krčo, Marko

    2003-04-01

    Observations of Koronis asteroid family members (158) Koronis, (277) Elvira, (311) Claudia, (321) Florentina, and (720) Bohlinia made during the period 1998-2001 yielded 61 new individual rotation lightcurves to augment previous surveys (R.P. Binzel, 1987, Icarus 72, 135-208; S.M. Slivan, R.P. Binzel, 1996, Icarus 124, 452-470) and allow determination of the senses of rotation and spin vector orientations for these objects. Spin vector reductions were performed on these five objects and also on family members (167) Urda, (208) Lacrimosa, (534) Nassovia, and (1223) Neckar using both a combination of amplitude-magnitude and epoch methods and a convex inversion method. A total of 213 individual lightcurves were analyzed to determine sidereal rotation periods, pole solutions and obliquities, associated photometric parameters, and model shapes for each object. We checked our methods and results using the (243) Ida Master Dataset of lightcurves (R. P. Binzel et al., 1993, Icarus 105, 310-325) and found that the true pole determined from the Galileo fly by of this irregularly shaped member of the Koronis family falls just at the edge of the estimated uncertainty of our own solution. Our findings for the spin vector distribution of 10 members within the Koronis family represent the first systematic study of spin states within a well-established Hirayama family, and provide observational constraints for models of the physics of family formation and spin vector evolution in the main belt.

  4. Report of a consultants meeting on draft guidelines for WWER 440/213 containment evaluation

    This report has been prepared at two Consultants' Meetings of experts from France, Germany, Russia, Slovakia, Spain and Ukraine, organized within the framework of the IAEA Extrabudgetary Programme on the Safety of WWER NPPs. In view of the limited time available and the complexity of the task the present report should be treated as a draft, which will be sent to the national regulatory bodies for review and further improvement. The next meeting on the subject will be held in June 1996 with the participation of representatives of all countries operating WWER 440/213 reactors with bubbler condenser containments. During the meeting, the acceptance criteria and review requirements formulated in the IAEA NUSS Series and those in force in the Russian Federation, the United States of America, Germany, France, the Czech Republic, and Slovakia have been taken into consideration. The equivalence of the technical goals which are aimed to be achieved by applying comparable national criteria has been checked in order to avoid multiple addressing the issues and to assure the completeness of the acceptance criteria necessary to assess the design of the containment systems. 48 refs, 12 tabs

  5. Cores, filaments, and bundles: hierarchical core formation in the L1495/B213 Taurus region

    Hacar, A; Kauffmann, J; Kovacs, A

    2013-01-01

    (Abridged) Context. Core condensation is a critical step in the star-formation process, but is still poorly characterized observationally. Aims. We have studied the 10 pc-long L1495/B213 complex in Taurus to investigate how dense cores have condensed out of the lower-density cloud material. Results. From the N$_2$H$^+$ emission, we identify 19 dense cores, some starless and some protostellar. They are not distributed uniformly, but tend to cluster with relative separations on the order of 0.25 pc. From the C$^{18}$O emission, we identify multiple velocity components in the gas. We have characterized them by fitting gaussians to the spectra, and by studying the distribution of the fits in position-position-velocity space. In this space, the C$^{18}$O components appear as velocity-coherent structures, and we have identified them automatically using a dedicated algorithm (FIVe: Friends In Velocity). Using this algorithm, we have identified 35 filamentary components with typical lengths of 0.5 pc, sonic internal ...

  6. Optoelectronic properties of fluorene-co-4,7-difuran-2,1,3-benzothiadiazole copolymers

    2010-01-01

    Light-emitting and photovoltaic properties of copolymers(PFO-FBT) based on 9,9-dioctylfluorene and 4,7-difuran-2,1,3-benzothiadiazole(FBT) units were investigated.The copolymers show two absorbance peaks at around 382 nm and 530 nm,respectively.The relative absorbance at around 530 nm of the PFO-FBT copolymers increases with the increasing FBT content.The EL emissions are red-shifted from 611 nm to 702 nm by increasing the FBT content in the copolymer.The highest EL external quantum efficiency achieved with the device configuration of ITO/PEDOT/PVK/PFO-FBT0.1/Ba/Al is 2.32% with the luminance of 441.3 cd/m2 at a current density of 33 mA/cm2.Efficient energy transfer due to exciton trapping on low band-gap FBT sites was observed.The bulk-heterojunction photovoltaic cell used PFO-FBT50 copolymer as the electron donor and methanofullerene [6,6]-phenyl C61-butyric acid methyl ester(PCBM) as the electron acceptor shows an energy conversion efficiency of 1.13% with open-circuit voltage(Voc) of 0.85 V and short-circuit current density(Jsc) of 3.39 mA/cm2 under AM 1.5 solar simulator(100 mW/cm2),and the edge of the spectral photoresponse is at ~750 nm.

  7. VERITAS Observations of the Unidentified Point Source HESS J1943+213

    ,

    2015-01-01

    The H.E.S.S. Galactic plane scan has revealed a large population of Galactic very high energy (VHE; E > 100 GeV) emitters. The majority of the galactic sources are extended and can typically be associated with pulsar wind nebulae (35%) and supernova remnants (21%), while some of the sources remain unidentified (31%). A much smaller fraction of point-like sources (5 in total, corresponding to 4%) are identified as gamma-ray binaries. Active galactic nuclei located behind the Galactic plane are also a potential source class. An active galaxy could be identified in the VHE regime by a point like extension, a high variability amplitude (up to a factor of 100) and a typically soft spectrum (due to absorption by the extra-galactic background light). Here we report on VERITAS observations of HESS J1943+213, an unidentified point source discovered to emit above 470 GeV during the extended H.E.S.S. Galactic plane scan. This source is thought to be a distant BL Lac object behind the Galactic plane and, though it exhibi...

  8. Unfolding the neutron spectrum of a NE213 scintillator using artificial neural networks

    Artificial neural networks technology has been applied to unfold the neutron spectra from the pulse height distribution measured with NE213 liquid scintillator. Here, both the single and multi-layer perceptron neural network models have been implemented to unfold the neutron spectrum from an Am-Be neutron source. The activation function and the connectivity of the neurons have been investigated and the results have been analyzed in terms of the network's performance. The simulation results show that the neural network that utilizes the Satlins transfer function has the best performance. In addition, omitting the bias connection of the neurons improve the performance of the network. Also, the SCINFUL code is used for generating the response functions in the training phase of the process. Finally, the results of the neural network simulation have been compared with those of the FORIST unfolding code for both 241Am-Be and 252Cf neutron sources. The results of neural network are in good agreement with FORIST code.

  9. The 1:1 adduct of caffeine and 2-(1,3-dioxoisoindolin-2-yl)acetic acid

    Bhatti, Moazzam H.; Yunus, Uzma; Saeed, Sohail; Shah, Syed Raza; Wong, Wing-Tak

    2011-01-01

    In the crystal structure of the title adduct [systematic name: 2-(1,3-dioxoisoindolin-2-yl)acetic acid–1,3,7-trimethyl-1,2,3,6-tetra­hydro-7H-purine-2,6-dione (1/1)], C8H10N4O2·C10H7NO4, the components are linked by an O—H⋯N hydrogen-bond and no proton transfer occurs. PMID:22058908

  10. The 1:1 adduct of caffeine and 2-(1,3-dioxoisoindolin-2-yl)acetic acid

    Bhatti, Moazzam H.; Uzma Yunus; Sohail Saeed; Syed Raza Shah; Wing-Tak Wong

    2011-01-01

    In the crystal structure of the title adduct [systematic name: 2-(1,3-dioxoisoindolin-2-yl)acetic acid–1,3,7-trimethyl-1,2,3,6-tetrahydro-7H-purine-2,6-dione (1/1)], C8H10N4O2·C10H7NO4, the components are linked by an O—H...N hydrogen-bond and no proton transfer occurs.

  11. Methemoglobin-forming effect and its role in the mechanism of action of some radioprotectors of benzo-2,1,3-thiadiazole class

    The ability of some benzo-2,1,3-tiadiazole derivatives to form methemoglobin in blood has been investigated. It has been shown that 30 min after intraperitoneal administration to mice of radioprotective doses of the preparations, the methemoglobin level in blood does not exceed 5%. After administration of a toxic dose of 4-aminobenzo-2,1,3-tiadiazole, the amount of methemoglobin in the mouse blood makes 14%. After per os administration of 4-aminobenzo-2,1,3-tiadiazole to dogs methemoglobin content of blood makes almost 50% which can provide a radioprotective action of this compound. Using substances preventing oxidation of hemoglobin to methemoglobin (isonicotinic acid hydrazide and 2-methoxy-9-phenazin sulphate) it has been shown that methemoglobin, formed in the mouse blood, does not play a leading part in manifestation of radioprotective and toxic properties of 4-aminobenzo-2,1,3-tiadiazole

  12. 1,213 Cases of Treatment of Facial Acne Using Indocyanine Green and Intense Pulsed Light in Asian Skin

    Kui Young Park

    2015-01-01

    Full Text Available Background. Photodynamic therapy (PDT has been used for acne, with various combinations of photosensitizers and light sources. Objective. We evaluated the effectiveness and safety of indocyanine green (ICG and intense pulsed light (IPL in the treatment of acne. Materials and Methods. A total of 1,213 patients with facial acne were retrospectively reviewed. Patients received three or five treatments of ICG and IPL at two-week intervals. Clinical response to treatment was assessed by comparing pre- and posttreatment clinical photographs and patient satisfaction scores. Results. Marked to excellent improvement was noted in 483 of 1,213 (39.8% patients, while minimal to moderate improvement was achieved in the remaining 730 (60.2% patients. Patient satisfaction scores revealed that 197 (16.3% of 1,213 patients were highly satisfied, 887 (73.1% were somewhat satisfied, and 129 (10.6% were unsatisfied. There were no significant side effects. Conclusion. These results suggest that PDT with ICG and IPL can be effectively and safely used in the treatment of acne.

  13. The status of the Bubbler Condenser Containment System for the Reactors of the VVER-440/213 Type

    VVER-440/213 Pressurized Water Reactors have a pressure-suppression containment structure called a 'Bubbler Condenser' tower which can reduce the design pressure of the entire containment following a design basis accident (DBA), such as a loss-of-coolant accident (LOCA). The bubbler condenser pressure suppression system provides reduction of the LOCA containment pressure by the condensation of released steam in a water pool. World-wide there are 14 nuclear power plants of the VVER-440/213 type in Eastern Europe and Russia. One of the safety concerns for the VVER-440/213 reactors relates to the ability of the bubbler condenser containment system to function satisfactorily and to maintain its integrity following certain postulated accidents and thus limit the release of radioactive material to the environment. The complicated geometry of the bubbler condenser unit, and the dependence on several moving devices and interlocks are the main doubts expressed by different specialists with regard to the design. General description of the bubbler condenser containment system, the physical processes, concerns and design assessment of the bubbler condenser containment system, presentation of the OECD's Unified Bubbler Condenser Research Project (UBCRP) and the European Commission PHARE/TACIS project. Recent utility investigations are also discussed

  14. Design basis and design features of WWER-440 model 213 nuclear power plants. Reference plant: Bohunice V2 (Slovakia)

    The prime objective of the IAEA Technical Co-operation Project on Evaluation of Safety Aspects of WWER-440 model 213 NPPs is to co-ordinate and to integrate assistance to national organizations in studying selected aspects of safety for the same type of reactors. Consequently, the study integrated the results generated by national activities carried out in the Czech Republic, Hungary, Slovakia and Ukraine and co-ordinated through the IAEA. Valuable assistance in carrying out the tasks was also provided by Bulgaria and Poland. A set of publications is being prepared to present the results of the project. The publications are intended to facilitate the review and utilization of the results of the project. They are also providing assistance in further refinement and/or extension of plant specific safety evaluation of model 213 NPPs. This Technical Document addressing the design basis and safety related design features of WWER-440 model 213 plants is the first of the series to be published. It is hoped that this document will be useful to anyone working in the field of WWER safety, and in particular to experts planning, executing or reviewing studies related to the subject. Refs, 36 figs, tabs

  15. On the Wegener granulomatosis associated region on chromosome 6p21.3

    Csernok Elena

    2006-03-01

    Full Text Available Abstract Background Wegener granulomatosis (WG belongs to the heterogeneous group of systemic vasculitides. The multifactorial pathophysiology of WG is supposedly caused by yet unknown environmental influence(s on the basis of genetic predisposition. The presence of anti-neutrophil cytoplasmic antibodies (ANCA in the plasma of patients and genetic involvement of the human leukocyte antigen system reflect an autoimmune background of the disease. Strong associations were revealed with WG by markers located in the major histocompatibility complex class II (MHC II region in the vicinity of human leukocyte antigen (HLA-DPB1 and the retinoid X receptor B (RXRB loci. In order to define the involvement of the 6p21.3 region in WG in more detail this previous population-based association study was expanded here to the respective 3.6 megabase encompassing this region on chromosome 6. The RXRB gene was analysed as well as a splice-site variation of the butyrophilin-like (BTNL2 gene which is also located within the respective region. The latter polymorphism has been evaluated here as it appears as a HLA independent susceptibility factor in another granulomatous disorder, sarcoidosis. Methods 150–180 German WG patients and a corresponding cohort of healthy controls (n = 100–261 were used in a two-step study. A panel of 94 microsatellites was designed for the initial step using a DNA pooling approach. Markers with significantly differing allele frequencies between patient and control pools were individually genotyped. The RXRB gene was analysed for single strand conformation polymorphisms (SSCP and restriction fragment length polymorphisms (RFLP. The splice-site polymorphism in the BTNL2 gene was also investigated by RFLP analysis. Results A previously investigated microsatellite (#1.0.3.7, Santa Cruz genome browser (UCSC May 2004 Freeze localisation: chr6:31257596-34999883, which was used as a positive control, remained associated throughout the whole two

  16. Thermal hydraulic analyses of NPPs with VVER-440/213 for the PTS condition evaluation

    The paper presents the Nuclear Research Institute (NRI) Rez approach to selection of accident scenarios with risk of pressurized thermal shock (PTS) on reactor pressure vessel (RPV) and, therefore, loss of the RPV integrity. Some thermal hydraulic analyses of the phenomenon performed so far are described and main results are shown. Suitability of different types of thermal hydraulic computer codes to such analyses is discussed. Planned activities are also presented. Attention is focused to thermal hydraulics; problems of fracture mechanic analyses which naturally follow the thermal hydraulic analyses and give the final answer to the question of severity of given accident from the point of view of PTS are not treated in this paper. Identification of accident scenarios involving a risk of PTS is the first step of the PTS thermal-hydraulic and fracture mechanic analyses. The paper contains a discussion of such scenarios for NPPs with VVER-440/213 reactors. Attention is paid mainly to secondary circuit leakages. As an example, one accident scenario (main steam header rupture with multiple steamline break) is analysed by means of RELAP5 code, then the reactor downcomer and lower plenum are analysed by means of the CATHARE 2 vl.3 two-dimensional module, and, finally, velocity and temperature fields in one cold leg and a part of the reactor downcomer are calculated by means of 3-D thermal-hydraulic code FLUTAN 2 for 50 seconds after start of HPIS pumps and the case of primary coolant stagnation. Time dependent development of a cold plume in the calculational region is shown at various cross-sections. (author)

  17. Optic toxicity in radiation treatment of meningioma: a retrospective study in 213 patients.

    Farzin, Mostafa; Molls, Michael; Kampfer, Severin; Astner, Sabrina; Schneider, Ralf; Roth, Karin; Dobrei, Michaela; Combs, Stephanie; Straube, Christoph

    2016-05-01

    In this retrospective evaluation, we correlated radiation dose parameters with occurrence of optical radiation-induced toxicities. 213 meningioma patients received radiation between 2000 and 2013. Radiation dose and clinical data were extracted from planning systems and patients' files. The range of follow-up period was 2-159 months (median 75 months). Median age of patients was 60 years (range 23-86). There were 163 female and 50 male patients. In 140 cases, at least one of the neuro-optic structures (optic nerves and chiasm) was inside the irradiated target volumes. We found 15 dry eye (7 %) and 24 cataract (11.2 %) cases. Median dose to affected lachrymal glands was 1.47 Gy and median dose to affected lenses was 1.05 Gy. Age and blood cholesterol level in patients with cataract were significantly higher. Patients with dry eye were significantly older. Only two patients with visual problems attributable to radiation treatment were seen. They did not have any risk factors. Maximum and median delivered doses to neuro-optic structures were not higher than 57.30 and 54.60 Gy respectively. Low percentages of cases with radiation induced high grade optic toxicities show that modern treatment techniques and doses are safe. In very few patients with optic side effects, doses to organs at risk were higher than the defined constraint doses. This observation leads to the problem of additional risk factors coming into play. The role of risk factors and safety of higher radiation doses in high grade meningiomas should be investigated in more comprehensive studies. PMID:26852221

  18. Targeted alpha-therapy using [Bi-213]anti-CD20 as novel treatment option for radio- and chemoresistant non-Hodgkin lymphoma cells

    Roscher, Mareike; Hormann, Inis; Leib, Oliver; Marx, Sebastian; Moreno, Josue; Miltner, Erich; Friesen, Claudia

    2013-01-01

    Radioimmunotherapy (RIT) is an emerging treatment option for non-Hodgkin lymphoma (NHL) producing higher overall response and complete remission rates compared with unlabelled antibodies. However, the majority of patients treated with conventional or myeloablative doses of radiolabelled antibodies relapse. The development of RIT with alpha-emitters is attractive for a variety of cancers because of the high linear energy transfer (LET) and short path length of alpha-radiation in human tissue, allowing higher tumour cell kill and lower toxicity to healthy tissues. In this study, we investigated the molecular effects of the alpha-emitter Bi-213 labelled to anti-CD20 antibodies ([Bi-213]anti-CD20) on cell cycle and cell death in sensitive and radio-/chemoresistant NHL cells. [Bi-213]anti-CD20 induced apoptosis, activated caspase-3, caspase-2 and caspase-9 and cleaved PARP specifically in CD20-expressing sensitive as well as in chemoresistant, beta-radiation resistant and gamma-radiation resistant NHL cells. CD20 negative cells were not affected by [Bi-213]anti-CD20 and unspecific antibodies labelled with Bi-213 could not kill NHL cells. Breaking radio-/chemoresistance in NHL cells using [Bi-213]anti-CD20 depends on caspase activation as demonstrated by complete inhibition of [Bi-213]anti-CD20-induced apoptosis with zVAD.fmk, a specific inhibitor of caspases activation. This suggests that deficient activation of caspases was reversed in radioresistant NHL cells using [Bi-213]anti-CD20. Activation of mitochondria, resulting in caspase-9 activation was restored and downregulation of Bcl-xL and XIAP, death-inhibiting proteins, was found after [Bi-213]anti-CD20 treatment in radio-/chemosensitive and radio-/chemoresistant NHL cells. [Bi-213]anti-CD20 seems to be a promising radioimmunoconjugate to improve therapeutic success by breaking radio- and chemoresistance selectively in CD20-expressing NHL cells via re-activating apoptotic pathways through reversing deficient

  19. Treatment of small cell lung cancer with a combination of VP16-213 and cyclophosphamide with cisplatin or radiotherapy

    Fifty five patients with small cell lung cancer were treated with a VP16-213 combination chemotherapy regimen in two consecutive series. The first series included 24 patients; 10 with limited and 14 with extensive disease were treated with VP16-213, 120 mg/m2 p.o. daily for 5 consecutive days, Cyclophosphamide 600 mg/m2 i.v. and Cisplatin 80 mg/m2 i.v. with hydratation and manitol induced diuresis. The cycle was repeated every 3 weeks. The second series included 31 similar patients, 16 limited, and 15 extensive disease, treated with VP16-213 at the same dose and Cyclophosphamide at 1,200 mg/m2 i.v. also repeated every 3 weeks; after three cycles the patients were treated with radiotherapy to the primary tumor and regional lymph nodes with 4,000 rads in a split course of three weeks interval, followed by the same combination chemotherapy. Response rate was 75% for the first series with 6 of 24 (25%) of complete responses in four limited and two extensive disease and a median survival time of 24 weeks. In the second series of patients there were 26 of 31 (83.8%) responses with 10 of 31 (32%) complete responses in nine limited and one extensive disease and a median survival time of 33 weeks for responders. Duration of response for complete responders was 36.8 weeks for the first series and 51 weeks for the second. Toxicity was mild and indludes nausea and vomitting, myelosupression, alopecia in both series, with one toxic death in the second series. Both regimes are active with a low complete response rate, which was increased in the second series by the addition of radiotherapy, which did not increase overall survival. (orig.)

  20. Evaluation of pressurised thermal shocks for VVER 440/213 reactor pressure vessel in NPP Dukovany

    NPP Dukovany is one of two NPPs operated in the Czech Republic. It has 4 units with VVER 440 type 213 reactors. The NPP started its operation in 1985 - 1987. PTS evaluation for this NPP was started in 1996 and the project is to be completed in 2004. Deterministic approach based on system thermal hydraulic analyses, detailed mixing analyses, and linear fracture mechanics is used. Evaluations are performed according to IAEA 'Guidelines on PTS Analysis for WWER NPP' and Czech standards. The following categories of PTS transients have been evaluated until now: (1) Large breaks in Main Steam System; (2) Leak from primary circuit through the pressurizer safety valve; (3) Spurious signals; (4) LOCA; (5) Primary to secondary circuit leaks. (A) Thermal Hydraulic Analyses: System TH analyses are performed using the RELAP5 computer code with a detailed 6-loop input model of NPP Dukovany. Primary and secondary circuits are modelled in detail according to the actual NPP configuration, signals specific to NPP Dukovany are built into the model and operator actions are taken into account for some PTS transients. The behaviour of the hermetic confinement, sumps and ECCS coolers is investigated by MELCOR code for LOCA accidents to determine ECCS water temperature in recirculation regime. The detailed mixing analyses are performed for all non-symmetric cases using CATHARE (with 2-D model of reactor downcomer) or REMIX/NEWMIX codes. (B) Structural Analyses: All computations are focused on circumferential weld 0.1.4 in belt-line zone. Overall approach to the analyses: Temperature and stress fields are computed by FEM on RPV model without crack. Linear elastic model is used. After that, the stress intensity factors KI are computed for postulated defects based on calculated stresses and using formulae from Czech standards. The defects are postulated (according to the IAEA Guidelines) as semi-elliptical surface cracks, both axial and circumferential, with aspect ratios a/c = 0,3 and 0

  1. NE-SPEC, Ne-213 Liquid Scintillation Detector Fast Neutron Spectra Unfolding

    1 - Description of problem or function: The NE-SPEC system is composed of three types of programs: - The code to convert the pulse height distributions from counts per channel to counts per light unit. - The code to generate the response matrix. - The unfolding code(s). 2 - Method of solution: Considering the three code types in turn: As the unit of the pulse height distribution must be matched to that of the response matrix, it is necessary to convert from counts per channel to counts per light unit. In this system the light unit used is the Na light unit. Also a new calibration method is used to determine the coefficients of the linear expression that defines the relationship between the channel number and the light unit. This method utilizes the relations between the values of the light unit for the energy of the Compton peak and the corresponding channel number of the gamma ray sources. The response matrix of the NE213 is obtained using Monte Carlo. 47 neutron groups are used in the test run, with cross sections from ENDF/B-IV. The light output data is that evaluated by Verbinski et al. The response matrix is reduced to 34 groups for the unfolding. The pulse height meshes are arranged at equal log spaces from 0.001 to 10.0 Na light units with 60 mesh points per decade. For unfolding, two codes, FERDOS and ITEM-II, which are based on different principles are available, so as to check their results. FERDOS employs a constrained least squares unfolding procedure whilst ITEM-II uses an iterative unfolding method. 3 - Restrictions on the complexity of the problem: Number of data points per unit decade of pulse height intervals - max. 60. Number of decades in the pulse height region: max. 4. Pulse height of initial mesh in Na units: min. 0.001. Number of channels in the measured pulse height distribution: max. 1024. Number of data points of the pulse height distribution: max. 150. Maximum size of response matrix is 55 (neutron energy mesh)*165 (light output mesh)

  2. Investigation of coolant mixing in WWER-440/213 RPV with improved turbulence model

    A detailed and complex RPV model of WWER-440/213 type reactor was developed in Budapest University of Technology and Economics Institute of Nuclear Techniques in the previous years. This model contains the main structural elements as inlet and outlet nozzles, guide baffles of hydro-accumulators coolant, alignment drifts, perforated plates, brake- and guide tube chamber and simplified core. With the new vessel model a series of parameter studies were performed considering turbulence models, discretization schemes, and modeling methods with ANSYS CFX. In the course of parameter studies the coolant mixing was investigated in the RPV. The coolant flow was 'traced' with different scalar concentration at the inlet nozzles and its distribution was calculated at the core bottom. The simulation results were compared with PAKS NPP measured mixing factors data (available from FLOMIX project. Based on the comparison the SST turbulence model was chosen for the further simulations, which unifies the advantages of two-equation (kω and kε) models. The most widely used turbulence models are Reynolds-averaged Navier-Stokes models that are based on time-averaging of the equations. Time-averaging filters out all turbulent scales from the simulation, and the effect of turbulence on the mean flow is then re-introduced through appropriate modeling assumptions. Because of this characteristic of SST turbulence model a decision was made in year 2011 to investigate the coolant mixing with improved turbulence model as well. The hybrid SAS-SST turbulence model was chosen, which is capable of resolving large scale turbulent structures without the time and grid-scale resolution restrictions of LES, often allowing the use of existing grids created for Reynolds-averaged Navier-Stokes simulations. As a first step the coolant mixing was investigated in the downcomer only. Eddies are occurred after the loop connection because of the steep flow direction change. This turbulent, vertiginous flow was

  3. Search for the decay K+ to pi+ gamma gamma in the pi+ momentum region P>213 MeV/c

    Artamonov, A V; Bassalleck, B; Bhuyan, B; Blackmore, E W; Bryman, D A; Chen, S; Chiang, I H; Christidi, I A; Cooper, P S; Diwan, M V; Frank, J S; Fujiwara, T; Hu, J; Ivashkin, A P; Jaffe, D E; Kabe, S; Kettell, S H; Khabibullin, M M; Khotjantsev, A N; Kitching, P; Kobayashi, M; Komatsubara, T K; Konaka, A; Kozhevnikov, A P; Kudenko, Yu G; Kushnirenko, A; Landsberg, L G; Lewis, B; Li, K K; Littenberg, L S; MacDonald, J A; Mildenberger, 3J; Mineev, O V; Miyajima, M; Mizouchi, K; Mukhin, V A; Muramatsu, N; Nakano, 4T; Nomachi, M; Nomura, T; Numao, T; Obraztsov, V F; Omata, K; Patalakha, D I; Petrenko, S V; Poutissou, R; Ramberg, E J; Redlinger, G; Sato, T; Sekiguchi, T; Shinkawa, T; Strand, R C; Sugimoto, S; Tamagawa, Y; Tschirhart, R S; Tsunemi, T; Vavilov, D V; Viren, B; Yershov, N V; Yoshimura, Y; Yoshioka, T

    2005-01-01

    We have searched for the K+ to pi+ gamma gamma decay in the kinematic region with pi+ momentum close to the end point. No events were observed, and the 90% confidence-level upper limit on the partial branching ratio was obtained, B(K+ to pi+ gamma gamma, P>213 MeV/c) < 8.3 x 10-9 under the assumption of chiral perturbation theory including next-to-leading order ``unitarity'' corrections. The same data were used to determine an upper limit on the K+ to pi+ gamma branching ratio of 2.3 x 10-9 at the 90% confidence level.

  4. Salmonella Typhimurium ST213 is associated with two types of IncA/C plasmids carrying multiple resistance determinants

    Zaidi Mussaret B

    2011-01-01

    Full Text Available Abstract Background Salmonella Typhimurium ST213 was first detected in the Mexican Typhimurium population in 2001. It is associated with a multi-drug resistance phenotype and a plasmid-borne blaCMY-2 gene conferring resistance to extended-spectrum cephalosporins. The objective of the current study was to examine the association between the ST213 genotype and blaCMY-2 plasmids. Results The blaCMY-2 gene was carried by an IncA/C plasmid. ST213 strains lacking the blaCMY-2 gene carried a different IncA/C plasmid. PCR analysis of seven DNA regions distributed throughout the plasmids showed that these IncA/C plasmids were related, but the presence and absence of DNA stretches produced two divergent types I and II. A class 1 integron (dfrA12, orfF and aadA2 was detected in most of the type I plasmids. Type I contained all the plasmids carrying the blaCMY-2 gene and a subset of plasmids lacking blaCMY-2. Type II included all of the remaining blaCMY-2-negative plasmids. A sequence comparison of the seven DNA regions showed that both types were closely related to IncA/C plasmids found in Escherichia, Salmonella, Yersinia, Photobacterium, Vibrio and Aeromonas. Analysis of our Typhimurium strains showed that the region containing the blaCMY-2 gene is inserted between traA and traC as a single copy, like in the E. coli plasmid pAR060302. The floR allele was identical to that of Newport pSN254, suggesting a mosaic pattern of ancestry with plasmids from other Salmonella serovars and E. coli. Only one of the tested strains was able to conjugate the IncA/C plasmid at very low frequencies (10-7 to 10-9. The lack of conjugation ability of our IncA/C plasmids agrees with the clonal dissemination trend suggested by the chromosomal backgrounds and plasmid pattern associations. Conclusions The ecological success of the newly emerging Typhimurium ST213 genotype in Mexico may be related to the carriage of IncA/C plasmids. We conclude that types I and II of Inc

  5. Upgrade the startup test instrumentation (ANMS) with innovated I and C system at the Dukovany WWER 440/213 units

    The paper focuses on the innovation procedures of physics startup tests instrumentation used in Dukovany NPP (ANMS) connected with I and C system refurbishment of the Dukovany WWER 440/213 units. The following matters are further discussed in this paper: a) changes in ANMS design, whose has been carried out due to reflect the situation connected with I and C innovation; b) process of consistency checking during implementation stage; c) results of the physics startup tests with upgraded ANMS in 2005 (introduction of Gd2 fuel on Dukovany NPP) (Authors)

  6. Therapeutic efficacy and toxicity of {sup 225}Ac-labelled vs. {sup 213}Bi-labelled tumour-homing peptides in a preclinical mouse model of peritoneal carcinomatosis

    Essler, Markus; Gaertner, Florian C.; Blechert, Birgit; Senekowitsch-Schmidtke, Reingard; Seidl, Christof [Technische Universitaet Muenchen, Department of Nuclear Medicine, Munich (Germany); Neff, Frauke [Helmholtz Zentrum Muenchen, Institute of Pathology, Neuherberg (Germany); Bruchertseifer, Frank; Morgenstern, Alfred [Institute for Transuranium Elements, European Commission, Joint Research Centre, Karlsruhe (Germany)

    2012-04-15

    Targeted delivery of alpha-particle-emitting radionuclides is a promising novel option in cancer therapy. We generated stable conjugates of the vascular tumour-homing peptide F3 both with {sup 225}Ac and {sup 213}Bi that specifically bind to nucleolin on the surface of proliferating tumour cells. The aim of our study was to determine the therapeutic efficacy of {sup 225}Ac-DOTA-F3 in comparison with that of {sup 213}Bi-DTPA-F3. ID{sub 50} values of {sup 213}Bi-DTPA-F3 and {sup 225}Ac-DOTA-F3 were determined via clonogenic assays. The therapeutic efficacy of both constructs was assayed by repeated treatment of mice bearing intraperitoneal MDA-MB-435 xenograft tumours. Therapy was monitored by bioluminescence imaging. Nephrotoxic effects were analysed by histology. ID{sub 50} values of {sup 213}Bi-DTPA-F3 and {sup 225}Ac-DOTA-F3 were 53 kBq/ml and 67 Bq/ml, respectively. The median survival of control mice treated with phosphate-buffered saline was 60 days after intraperitoneal inoculation of 1 x 10{sup 7} MDA-MB-435 cells. Therapy with 6 x 1.85 kBq of {sup 225}Ac-DOTA-F3 or 6 x 1.85 MBq of {sup 213}Bi-DTPA-F3 prolonged median survival to 95 days and 97 days, respectively. While F3 labelled with short-lived {sup 213}Bi (t{sub 1/2} 46 min) reduced the tumour mass at early time-points up to 30 days after treatment, the antitumour effect of {sup 225}Ac-DOTA-F3 (t{sub 1/2} 10 days) increased at later time-points. The difference in the fraction of necrotic cells after treatment with {sup 225}Ac-DOTA-F3 (43%) and with {sup 213}Bi-DTPA-F3 (36%) was not significant. Though histological analysis of kidney samples revealed acute tubular necrosis and tubular oedema in 10-30% of animals after treatment with {sup 225}Ac-DOTA-F3 or {sup 213}Bi-DTPA-F3, protein casts were negligible (2%), indicating only minor damage to the kidney. Therapy with both {sup 225}Ac-DOTA-F3 and {sup 213}Bi-DTPA-F3 increased survival of mice with peritoneal carcinomatosis. Mild renal toxicity of both

  7. Strength analyses of the bubbler condenser structure of WWER-440 model 213 nuclear power plants. Reference plant: Bohunice V2 (Slovakia). Report of the IAEA Technical Co-operation Project RER/9/004 on evaluation of safety aspects of WWER-440 model 213 nuclear power plants

    This publication addresses the topic of mechanical strength of the bubbler condenser applied in the WWER-440 model 213 plants and is intended to assist WWER-440/213 operators in the re-assessment of the bubbler condenser performance. It is hoped that it will also be useful to anyone working in the field of WWER safety, and in particular to experts planning, executing or reviewing studies related to the subject. Refs, figs and tabs

  8. WWER 440/213 NPP containment from the point of view of IAEA requirements and current European practice

    In principle, in a NPP three barriers are used to prevent the release of radioactive substances into the environment: the fuel cladding, the primary circuit boundary, and the containment. The presentation deals with the third barrier - the containment, and explains the philosophy of maximum design accident management in the containment of WWER-440/213 NPPs. This type of containment is shown to be an original and fully functional technical solution. Due to the use of the large reserve of the H3BO3 solution and to the active spray systems, an underpressure can be quickly established, thus minimizing any impact of the accident on the environment. The capability of the bubble condenser system for a maximum design accident has been proven by analyses and by tests performed within the framework of PHARE PH 2.13/95: the condenser system can reduce the pressure in the containment in an effective way. The majority of questions arising so far have been answered and the concern resulting from insufficient verification of the system with regard to western standards has been refuted. The WWER-440/213 is fully consistent with IAEA requirements and with current European practice. (A.K.)

  9. Tratamiento quirúrgico de las cardiopatías congénitas: resultados de 213 procedimientos consecutivos

    Diego Abdala

    2008-06-01

    Full Text Available Dado el progreso del tratamiento quirúrgico de las cardiopatías congénitas, y el predominio de las cirugías correctivas con respecto a las paliativas, hemos querido evaluar los resultados quirúrgicos, en forma individual y comparativa, de 213 procedimientos quirúrgicos por cardiopatía congénita realizados en un único centro de nuestro país. Material y métodos: se revisaron retrospectivamente los registros médicos y quirúrgicos de la base de datos del Centro Cardiológico Americano, identificándose 213 cirugías cardíacas consecutivas por cardiopatía congénita en un período de 39 meses (desde enero de 2003 hasta marzo de 2006. Se analizó la morbimortalidad al alta, comparándose con la literatura internacional. Resultados: la mortalidad al alta fue de 7% y el porcentaje de complicaciones de 16%, 87,8% de los procedimientos fueron correctivos. Conclusiones: la mortalidad total al alta se encuentra dentro de los límites referidos en muchos de los estudios publicados. Hay una marcada tendencia en favorecer las cirugías correctivas. La mayor mortalidad la posee el grupo de pacientes recién nacidos con cardiopatías complejas.

  10. THE EFFECTS OF BOGUS TYPHOON AND OBSERVED OCEANIC DATA ON THE ABILITY OF T213L31 TO PREDICT TC TRACK

    MA Su-hong; WANG Jian-jie; WAN Feng

    2007-01-01

    The abilities of typhoon (TC) track prediction by a medium-range forecast model T213L31 at National Meteorological Center are analyzed and its ability to improve its TC forecasts is discussed. The results show that about 57% of the TCs could be predicted by T213L31 but the initial position errors are large.The 43% area without the prediction of TC tracks is concentrated between 13°N and 20°N and east of 120°E and lack of conventional observation data is the main reason for the absence of TC prediction in this area.The adding of bogus TC could improve the ability of TC track prediction when there is no TC vortex in the analysis field, but could only have positive effects on the short-range TC track prediction when there is TC vortex in the T213L31 analysis field.

  11. Anti-CD45 Pretargeted Radioimmunotherapy using Bismuth-213: High Rates of Complete Remission and Long-Term Survival in a Mouse Myeloid Leukemia Xenograft Model

    Pagel, John M; Kenoyer, Aimee L; Back, Tom; Hamlin, Donald K; Wilbur, D Scott; Fisher, Darrell R; Park, Steven I; Frayo, Shani; Axtman, Amanda; Orgun, Nural; Orozoco, Johnnie; Shenoi, Jaideep; Lin, Yukang; Gopal, Ajay K; Green, Damian J; Appelbaum, Frederick R; Press, Oliver W

    2011-07-21

    Pretargeted radioimmunotherapy (PRIT) using an anti-CD45 antibody (Ab)-streptavidin (SA) conjugate and DOTA-biotin labeled with β-emitting radionuclides has been explored as a strategy to decrease relapse and toxicity. α-emitting radionuclides exhibit high cytotoxicity coupled with a short path-length, potentially increasing the therapeutic index and making them an attractive alternative to β-emitting radionuclides for patients with Acute Myeloid Leukemia (AML). Accordingly, we have used 213Bi in mice with human leukemia xenografts. Results demonstrated excellent localization of 213Bi-DOTA-biotin to tumors with minimal uptake into normal organs. After 10 minutes, 4.5 ± 1.1% of the injected dose of 213Bi was delivered per gram of tumor. α imaging demonstrated uniform radionuclide distribution within tumor tissue 45 minutes after 213Bi-DOTA-biotin injection. Radiation absorbed doses were similar to those observed using a β-emitting radionuclide (90Y) in the same model. We conducted therapy experiments in a xenograft model using a single-dose of 213Bi-DOTA-biotin given 24 hours after anti-CD45 Ab-SA conjugate. Among mice treated with anti-CD45 Ab-SA conjugate followed by 800 μCi of 213Bi- or 90Y-DOTA-biotin, 80% and 20%, respectively, survived leukemia-free for >100 days with minimal toxicity. These data suggest that anti-CD45 PRIT using an α-emitting radionuclide may be highly effective and minimally toxic for treatment of AML.

  12. Targeted alpha-radionuclide therapy of functionally critically located gliomas with 213Bi-DOTA-[Thi8,Met(O2)11]-substance P: a pilot trial

    Functionally critically located gliomas represent a challenging subgroup of intrinsic brain neoplasms. Standard therapeutic recommendations often cannot be applied, because radical treatment and preservation of neurological function are contrary goals. The successful targeting of gliomas with locally injected beta radiation-emitting 90Y-DOTAGA-substance P has been shown previously. However, in critically located tumours, the mean tissue range of 5 mm of 90Y may seriously damage adjacent brain areas. In contrast, the alpha radiation-emitting radionuclide 213Bi with a mean tissue range of 81 μm may have a more favourable toxicity profile. Therefore, we evaluated locally injected 213Bi-DOTA-substance P in patients with critically located gliomas as the primary therapeutic modality. In a pilot study, we included five patients with critically located gliomas (WHO grades II-IV). After diagnosis by biopsy, 213Bi-DOTA-substance P was locally injected, followed by serial SPECT/CT and MR imaging and blood sampling. Besides feasibility and toxicity, the functional outcome was evaluated. Targeted radiopeptide therapy using 213Bi-DOTA-substance P was feasible and tolerated without additional neurological deficit. No local or systemic toxicity was observed. 213Bi-DOTA-substance P showed high retention at the target site. MR imaging was suggestive of radiation-induced necrosis and demarcation of the tumours, which was validated by subsequent resection. This study provides proof of concept that targeted local radiotherapy using 213Bi-DOTA-substance P is feasible and may represent an innovative and effective treatment for critically located gliomas. Primarily non-operable gliomas may become resectable with this treatment, thereby possibly improving the prognosis. (orig.)

  13. 213Bi-anti-EGFR radioimmunoconjugates and X-ray irradiation trigger different cell death pathways in squamous cell carcinoma cells

    Introduction: Treatment of patients with squamous cell carcinoma of head and neck is hampered by resistance of tumor cells to irradiation. Additional therapies enhancing the effect of X-ray irradiation may be beneficial. Antibodies targeting EGFR have been shown to improve the efficacy of radiation therapy. Therefore, we analyzed cytotoxicity of 213Bi-anti-EGFR immunoconjugates in combination with X-ray irradiation. Methods: The monoclonal anti-EGFR antibody matuzumab was coupled to CHX-A”-DTPA forming stable complexes with 213Bi. Cytotoxicity of X-ray radiation, of treatment with 213Bi-anti-EGFR monoclonal antibodies (MAb) or of a combined treatment regimen was assayed using cell proliferation and colony formation assays in UD-SCC5 cells. Key proteins of cell-cycle arrest and cell death were examined by Western blot analysis. Cell cycle analysis was performed by flow cytometry. DNA double-strand breaks were detected via γH2AX and quantified using Definiens™ software. Results: Irradiation with X-rays or treatment with 213Bi-anti-EGFR-MAb resulted in median lethal dose (LD50) values of 12 Gy or 130 kBq/mL, respectively. Treatment with 37 kBq/mL of 213Bi-anti-EGFR-MAb or 2 Gy of X-rays had only little effect on colony formation of UD-SCC5 cells. In contrast, a combined treatment regimen (37 kBq/mL plus 2 Gy) significantly decreased colony formation and enhanced the formation of DNA double-strand breaks. As revealed by flow cytometry, radiation treatments caused accumulation of cells in the G0/G1 phase. Both treatment with 213Bi-anti-EGFR immunoconjugates and application of the combined treatment regimen triggered activation of genes of signaling pathways involved in cell-cycle arrest and induction of apoptosis like p21/Waf, GADD45, Puma and Bax, which were only marginally modulated by X-ray irradiation of cells. Conclusions: 213Bi-anti-EGFR-MAb enhances cytotoxicity of X-ray irradiation in UD-SCC5 cells most probably due to effective induction of DNA double

  14. Main building complex WWER 440/213 upper range design response spectra for soft soil site conditions (Paks)

    Within the Benchmark studies parallel investigation were prepared for the main building complex of the WWER-440/213 Paks NPP by several participating institutions. The investigations were based on various mathematical models and procedures but all had the same seismological data as input. The calculation methods as well as software tools were different. This report covers the enveloped response results which were the basis for the benchmark studies and which should be used for upgrading of mechanical and electrical components and systems which will follow. These response spectra which consider a certain conservatism namely neglecting the frequency independence of the stiffness and the cut-off of damping values are named 'Upper Range design Benchmark Response Spectra' for the main building of Paks NPP

  15. Requirements and possible upgrading concept for the WWER-440/213: Mochovce NPP structures under seismic conditions

    The Mochovce-Nuclear Power Plant is one of the WWER-440/213 plants which has been designed against earthquake. Nevertheless, the design earthquake has not been assessed adequately to the seismic hazard at the site. A new seismic design shall include an increased seismic input and assure an acceptable standard of safety. This contribution is related to some design aspects of civil structures for this nearly finished plant, such as: existing design and its margins with regard to the employed codes; requirements for a new design concept; effects to be expected by an increased design earthquake; applicable design methods; use of inelastic design spectra, behavior factors and capacity design; feasible upgrading measures. (author)

  16. Focal cortical dysplasia, microcephaly and epilepsy in a boy with 1q21.1-q21.3 duplication.

    Milone, Roberta; Valetto, Angelo; Battini, Roberta; Bertini, Veronica; Valvo, Giulia; Cioni, Giovanni; Sicca, Federico

    2016-05-01

    The recent advance of new molecular technologies like array - Comparative Genomic Hybridization has fostered the detection of genomic imbalances in subjects with intellectual disability, epilepsy, and/or congenital anomalies. Though some of the rearrangements are relatively frequent, their consequences on phenotypes can be strongly variable. We report on a boy harbouring a de novo 8.3 Mb duplication of chromosome 1q21.1-q21.3 whose complex unusual phenotype deserves attention, due to the presence of focal cortical dysplasia, microcephaly, and epilepsy. Loss-of-function (LOF) effects of genes associated with human disease involved in the rearrangement have been only partially established, and have not been previously associated with brain malformations in several deletion syndromes. Less is known, instead, about the consequences of their duplication on neuronal migration and brain development process. Further advance in neuroimaging and genetic research will help in defining their actual role in neurodevelopment and cerebral cortex malformations. PMID:26975584

  17. Search for the decay K+ to pi+ gamma gamma in the pi+ momentum region P > 213 MeV/c

    Artamonov, A.V.; Bassalleck, B.; Bhuyan, B.; Blackmore, E.W.; Bryman, D.A.; Chen, S.; Chiang, I.-H.; Christidi, I.-A.; Cooper, P.S.; Diwan, M.V.; Frank, J.S.; Fujiwara,; Hu, J.; Jaffe, D.E.; Kabe, S.; Kettell, S.H.; Khabibullin, M.M.; Khotjantsev, A.N.; Kitching, P.; Kobayashi, M.; Komatsubara, T.K.; /Serpukhov, IHEP /New Mexico U. /Brookhaven

    2005-05-01

    We have searched for the K{sup +} {yields} {pi}{sup +}{gamma}{gamma} decay in the kinematic region with {pi}{sup +} momentum close to the end point. No events were observed, and the 90% confidence-level upper limit on the partial branching ratio was obtained, B(K{sup +} {yields} {pi}{sup +}{gamma}{gamma}, P > 213 MeV/c) < 8.3 x 10{sup -9} under the assumption of chiral perturbation theory including next-to-leading order ''unitarity'' corrections. The same data were used to determine an upper limit on the K{sup +} {yields} {pi}{sup +}{gamma} branching ratio of 2.3 x 10{sup -9} at the 90% confidence level.

  18. Relap5/Mod3.1 analysis of main steam header rupture in VVER- 440/213 NPP

    Kral, P. [Nuclear Research Inst. Rez (Switzerland)

    1995-12-31

    The presentation is focused on two main topics. First the applied modelling of PGV-4 steam generator for RELAP5 code are described. The results of steady-state calculation under reference conditions are compared against measured data. The problem of longitudinal subdivision of SG tubes is analysed and evaluated. Secondly, a best-estimate analysis of main steam header (MSH) rupture accident in WWER-440/213 NPP is presented. The low reliability of initiation of ESFAS signal `MSH Rupture` leads in this accident to big loss of secondary coolant, full depressurization of main steam system, extremely fast cool-down of both secondary and primary system, opening of PRZ SV-bypass valve with later liquid outflow, potential reaching of secondary criticality by failure of HPIS. 7 refs.

  19. Relap5/Mod3.1 analysis of main steam header rupture in VVER- 440/213 NPP

    The presentation is focused on two main topics. First the applied modelling of PGV-4 steam generator for RELAP5 code are described. The results of steady-state calculation under reference conditions are compared against measured data. The problem of longitudinal subdivision of SG tubes is analysed and evaluated. Secondly, a best-estimate analysis of main steam header (MSH) rupture accident in WWER-440/213 NPP is presented. The low reliability of initiation of ESFAS signal 'MSH Rupture' leads in this accident to big loss of secondary coolant, full depressurization of main steam system, extremely fast cool-down of both secondary and primary system, opening of PRZ SV-bypass valve with later liquid outflow, potential reaching of secondary criticality by failure of HPIS

  20. Repensando la libertad de expresión desde el abordaje al art. 213 del Código Penal argentino

    Matalone, Noelia

    2013-12-01

    Full Text Available Este ensayo intenta presentar abordajes críticos sobre el delito tipificado en el art. 213 del Código Penal. En tal temperamento, se contrapone el tipo penal de apología del delito con los derechos individuales de las personas, en particular, la libertad de expresión. En este sentido, la autora formula una propuesta de derogación de la norma, como consecuencia de los fundamentos y efectos de esta norma, todo ello en orden a preservar, por sobre los intereses que puedan sostener este tipo de prohibición, la pluralidad de voces en la sociedad. Para ello, apela al sentido de la tolerancia social y a los principios de razonalibidad y de necesidad del sistema penal al momento de investigar y perseguir este tipo de casos.

  1. Enhanced efficacy of combined {sup 213}Bi-DTPA-F3 and paclitaxel therapy of peritoneal carcinomatosis is mediated by enhanced induction of apoptosis and G2/M phase arrest

    Vallon, Mario; Seidl, Christof; Blechert, Birgit; Li, Zhoulei; Gaertner, Florian C.; Senekowitsch-Schmidtke, Reingard; Essler, Markus [Technische Universitaet Muenchen, Department of Nuclear Medicine, Munich (Germany); Gilbertz, Klaus-Peter [German Armed Forces, Institute of Radiobiology, Munich (Germany); Baumgart, Anja [Technische Universitaet Muenchen, III. Medical Department, Munich (Germany); Aichler, Michaela; Feuchtinger, Annette; Walch, Axel K. [Helmholtz Zentrum Muenchen, Institute of Pathology, Neuherberg (Germany); Bruchertseifer, Frank; Morgenstern, Alfred [Institute for Transuranium Elements, European Commission, Joint Research Centre, Karlsruhe (Germany)

    2012-12-15

    Targeted therapy with {alpha}-particle emitting radionuclides is a promising new option in cancer therapy. Stable conjugates of the vascular tumour-homing peptide F3 with the {alpha}-emitter {sup 213}Bi specifically target tumour cells. The aim of our study was to determine efficacy of combined {sup 213}Bi-diethylenetriaminepentaacetic acid (DTPA)-F3 and paclitaxel treatment compared to treatment with either {sup 213}Bi-DTPA-F3 or paclitaxel both in vitro and in vivo. Cytotoxicity of treatment with {sup 213}Bi-DTPA-F3 and paclitaxel, alone or in combination, was assayed towards OVCAR-3 cells using the alamarBlue assay, the clonogenic assay and flow cytometric analyses of the mode of cell death and cell cycle arrest. Therapeutic efficacy of the different treatment options was assayed after repeated treatment of mice bearing intraperitoneal OVCAR-3 xenograft tumours. Therapy monitoring was performed by bioluminescence imaging and histopathologic analysis. Treatment of OVCAR-3 cells in vitro with combined {sup 213}Bi-DTPA-F3 and paclitaxel resulted in enhanced cytotoxicity, induction of apoptosis and G2/M phase arrest compared to treatment with either {sup 213}Bi-DTPA-F3 or paclitaxel. Accordingly, i.p. xenograft OVCAR-3 tumours showed the best response following repeated (six times) combined therapy with {sup 213}Bi-DTPA-F3 (1.85 MBq) and paclitaxel (120 {mu}g) as demonstrated by bioluminescence imaging and histopathologic investigation of tumour spread on the mesentery of the small and large intestine. Moreover, mean survival of xenograft mice that received combined therapy with {sup 213}Bi-DTPA-F3 and paclitaxel was significantly superior to mice treated with either {sup 213}Bi-DTPA-F3 or paclitaxel alone. Combined treatment with {sup 213}Bi-DTPA-F3 and paclitaxel significantly increased mean survival of mice with peritoneal carcinomatosis of ovarian origin, thus favouring future therapeutic application. (orig.)

  2. Enhanced efficacy of combined 213Bi-DTPA-F3 and paclitaxel therapy of peritoneal carcinomatosis is mediated by enhanced induction of apoptosis and G2/M phase arrest

    Targeted therapy with α-particle emitting radionuclides is a promising new option in cancer therapy. Stable conjugates of the vascular tumour-homing peptide F3 with the α-emitter 213Bi specifically target tumour cells. The aim of our study was to determine efficacy of combined 213Bi-diethylenetriaminepentaacetic acid (DTPA)-F3 and paclitaxel treatment compared to treatment with either 213Bi-DTPA-F3 or paclitaxel both in vitro and in vivo. Cytotoxicity of treatment with 213Bi-DTPA-F3 and paclitaxel, alone or in combination, was assayed towards OVCAR-3 cells using the alamarBlue assay, the clonogenic assay and flow cytometric analyses of the mode of cell death and cell cycle arrest. Therapeutic efficacy of the different treatment options was assayed after repeated treatment of mice bearing intraperitoneal OVCAR-3 xenograft tumours. Therapy monitoring was performed by bioluminescence imaging and histopathologic analysis. Treatment of OVCAR-3 cells in vitro with combined 213Bi-DTPA-F3 and paclitaxel resulted in enhanced cytotoxicity, induction of apoptosis and G2/M phase arrest compared to treatment with either 213Bi-DTPA-F3 or paclitaxel. Accordingly, i.p. xenograft OVCAR-3 tumours showed the best response following repeated (six times) combined therapy with 213Bi-DTPA-F3 (1.85 MBq) and paclitaxel (120 μg) as demonstrated by bioluminescence imaging and histopathologic investigation of tumour spread on the mesentery of the small and large intestine. Moreover, mean survival of xenograft mice that received combined therapy with 213Bi-DTPA-F3 and paclitaxel was significantly superior to mice treated with either 213Bi-DTPA-F3 or paclitaxel alone. Combined treatment with 213Bi-DTPA-F3 and paclitaxel significantly increased mean survival of mice with peritoneal carcinomatosis of ovarian origin, thus favouring future therapeutic application. (orig.)

  3. 31 CFR 535.213 - Direction involving property held by offices of banks in the United States in which Iran or an...

    2010-07-01

    ... 535.213 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE OF... Iranian entity has an interest. (a) Any branch or office of a bank, which branch or office is located... rates are to be determined in the future, whether by agreement between Iran and the bank or...

  4. The AtRAD21.1 and AtRAD21.3 Arabidopsis cohesins play a synergistic role in somatic DNA double strand break damage repair

    da Costa-Nunes, J.A.; Capitao, C.; Kozák, Jaroslav; Costa-Nunes, P.; Ducasa, G.M.; Pontes, O.; Angelis, Karel

    2014-01-01

    Roč. 14, DEC 16 2014 (2014). ISSN 1471-2229 R&D Projects: GA MŠk(CZ) LD13006; GA ČR GA13-06595S Institutional support: RVO:61389030 Keywords : Arabidopsis * AtRAD21.1 * AtRAD21.3 Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.813, year: 2014

  5. {sup 213}Bi-DOTATOC receptor-targeted alpha-radionuclide therapy induces remission in neuroendocrine tumours refractory to beta radiation: a first-in-human experience

    Kratochwil, C.; Giesel, F.L.; Mier, W.; Haberkorn, U. [University Hospital Heidelberg, Department of Nuclear Medicine, Heidelberg (Germany); Bruchertseifer, F.; Apostolidis, C.; Morgenstern, A. [European Commission, Institute for Transuranium Elements, Karlsruhe (Germany); Boll, R.; Murphy, K. [Oak Ridge National Laboratory, Oak Ridge, TN (United States)

    2014-11-15

    Radiopeptide therapy using a somatostatin analogue labelled with a beta emitter such as {sup 90}Y/{sup 177}Lu-DOTATOC is a new therapeutic option in neuroendocrine cancer. Alternative treatments for patients with refractory disease are rare. Here we report the first-in-human experience with {sup 213}Bi-DOTATOC targeted alpha therapy (TAT) in patients pretreated with beta emitters. Seven patients with progressive advanced neuroendocrine liver metastases refractory to treatment with {sup 90}Y/{sup 177}Lu-DOTATOC were treated with an intraarterial infusion of {sup 213}Bi-DOTATOC, and one patient with bone marrow carcinosis was treated with a systemic infusion of {sup 213}Bi-DOTATOC. Haematological, kidney and endocrine toxicities were assessed according to CTCAE criteria. Radiological response was assessed with contrast-enhanced MRI and {sup 68}Ga-DOTATOC-PET/CT. More than 2 years of follow-up were available in seven patients. The biodistribution of {sup 213}Bi-DOTATOC was evaluable with 440 keV gamma emission scans, and demonstrated specific tumour binding. Enduring responses were observed in all treated patients. Chronic kidney toxicity was moderate. Acute haematotoxicity was even less pronounced than with the preceding beta therapies. TAT can induce remission of tumours refractory to beta radiation with favourable acute and mid-term toxicity at therapeutic effective doses. (orig.)

  6. 5 CFR 839.213 - May I make a retirement coverage election if I withdrew all or part of my TSP account after I was...

    2010-01-01

    ... of my TSP account after I was corrected to FERS? (a) You may not make a retirement coverage election... after you separated (because your account balance was $3,500 or less), or if you received a financial... if I withdrew all or part of my TSP account after I was corrected to FERS? 839.213 Section...

  7. A novel duplication of chromosome (13)(q14.1q21.3) in a patient with mental retardation and microcephaly.

    Verhoeven, W.; Ruiter, M.; Egger, J.; Tuinier, S.; Smeets, D.F.C.M.

    2009-01-01

    We report on a mentally retarded female with behavioural problems, microcephaly, mild facial dysmorphisms, short stature and small hands with thin fingers due to a de novo partial duplication within the long arm of chromosome 13(q14.1q21.3). She was primarily referred to the outpatient department of

  8. A novel duplication of chromosome (13)(q14.1q21.3) in a patient with mental retardation and microcephaly

    Verhoeven, W.M.A.; Ruiter, E.M.; Egger, J.I.M.; Tuinier, S.; Smeets, D.F.C.M.

    2009-01-01

    A novel duplication of chromosome (13)(q14.1q21.3) in a patient with mental retardation and microcephaly: We report on a mentally retarded female with behavioural problems, microcephaly, mild facial dysmorphisms, short stature and small hands with thin fingers due to a de novo partial duplication wi

  9. 213Bi-DOTATOC receptor-targeted alpha-radionuclide therapy induces remission in neuroendocrine tumours refractory to beta radiation: a first-in-human experience

    Radiopeptide therapy using a somatostatin analogue labelled with a beta emitter such as 90Y/177Lu-DOTATOC is a new therapeutic option in neuroendocrine cancer. Alternative treatments for patients with refractory disease are rare. Here we report the first-in-human experience with 213Bi-DOTATOC targeted alpha therapy (TAT) in patients pretreated with beta emitters. Seven patients with progressive advanced neuroendocrine liver metastases refractory to treatment with 90Y/177Lu-DOTATOC were treated with an intraarterial infusion of 213Bi-DOTATOC, and one patient with bone marrow carcinosis was treated with a systemic infusion of 213Bi-DOTATOC. Haematological, kidney and endocrine toxicities were assessed according to CTCAE criteria. Radiological response was assessed with contrast-enhanced MRI and 68Ga-DOTATOC-PET/CT. More than 2 years of follow-up were available in seven patients. The biodistribution of 213Bi-DOTATOC was evaluable with 440 keV gamma emission scans, and demonstrated specific tumour binding. Enduring responses were observed in all treated patients. Chronic kidney toxicity was moderate. Acute haematotoxicity was even less pronounced than with the preceding beta therapies. TAT can induce remission of tumours refractory to beta radiation with favourable acute and mid-term toxicity at therapeutic effective doses. (orig.)

  10. Penam sulfones and β-lactamase inhibition: SA2-13 and the importance of the C2 side chain length and composition.

    Elizabeth A Rodkey

    Full Text Available β-Lactamases are the major reason β-lactam resistance is seen in Gram-negative bacteria. To combat this resistance mechanism, β-lactamase inhibitors are currently being developed. Presently, there are only three that are in clinical use (clavulanate, sulbactam and tazobactam. In order to address this important medical need, we explored a new inhibition strategy that takes advantage of a long-lived inhibitory trans-enamine intermediate. SA2-13 was previously synthesized and shown to have a lower k(react than tazobactam. We investigated here the importance of the carboxyl linker length and composition by synthesizing three analogs of SA2-13 (PSR-4-157, PSR-4-155, and PSR-3-226. All SA2-13 analogs yielded higher turnover numbers and k(react compared to SA2-13. We next demonstrated using protein crystallography that increasing the linker length by one carbon allowed for better capture of a trans-enamine intermediate; in contrast, this trans-enamine intermediate did not occur when the C2 linker length was decreased by one carbon. If the linker was altered by both shortening it and changing the carboxyl moiety into a neutral amide moiety, the stable trans-enamine intermediate in wt SHV-1 did not form; this intermediate could only be observed when a deacylation deficient E166A variant was studied. We subsequently studied SA2-13 against a relatively recently discovered inhibitor-resistant (IR variant of SHV-1, SHV K234R. Despite the alteration in the mechanism of resistance due to the K→R change in this variant, SA2-13 was effective at inhibiting this IR enzyme and formed a trans-enamine inhibitory intermediate similar to the intermediate seen in the wt SHV-1 structure. Taken together, our data reveals that the C2 side chain linker length and composition profoundly affect the formation of the trans-enamine intermediate of penam sulfones. We also show that the design of SA2-13 derivatives offers promise against IR SHV β-lactamases that possess the

  11. Analysis of the inhibitory effects of VP-16-213 (etoposide) and podophyllotoxin on thymidine transport and metabolism in Ehrlich ascites tumor cells in vitro

    Uptake of 3H after exposure of cells to [3H]-thymidine is characterized by a rapid initial velocity that approximates membrane transport followed by a slower rate of uptake that parallels the accumulation of phosphorylated derivatives of thymidine, primarily thymidine triphosphate, within the cell. The high rate of thymidine transport relative to thymidine metabolism to the triphosphate within the cell decreases as the extracellular nucleoside concentration is reduced due to a much greater decrease in membrane transport than the subsequent metabolic step. Hence, as extracellular thymidine is decreased, transport becomes increasingly rate limiting to metabolism within the cell. VP-16-213 (etoposide) or podophyllotoxin inhibits the initial uptake rate for thymidine and, as a consequence, inhibits the intracellular formation of thymidine triphosphate. When extracellular thymidine is high, inhibitory effects on transport are transient, and the net rate of thymidine triphosphate accumulation within drug-treated cells rapidly approaches a velocity comparable to that of control cells, indicating no direct VP-16-213 or podophyllotoxin effect on nucleoside and nucleotide phosphorylation. When extracellular thymidine is reduced so that transport is rate limiting to metabolism, the duration of the inhibitory effects of VP-16-213 on thymidine triphosphate formation is prolonged. A secondary effect of VP-16-213 becomes manifest beyond 10 min of incubation with [3H]thymidine with the virtual complete cessation of thymidine incorporation into the acid precipitate without any change in the thymidine triphosphate level. This late effect is not observed with podophyllotoxin and indicates a direct effect of VP-16-213 on DNA synthesis that is distinct from the earlier inhibitory effect on thymidine phosphorylation, which is secondary to membrane transport

  12. Possible implication of LECAM-1 gene P213S polymorphism in the risk for advanced stages of diabetic nephropathy in patients with type 1 diabetes

    Nicolae Mircea PANDURU

    2009-11-01

    Full Text Available Diabetic nephropathy has an unclarified pathogenesis, with multifactorial aetiology which includes metabolic and haemodynamic abnormalities, aberrant signaling of numerous cytokines or growth factors and genetic susceptibility. Inflammation and low birth weight seems to be predisposing factors for diabetic nephropathy. In both processes levels of L-selectin (CD62L may play an important role. The genetic heritability of diabetic nephropathy and CD62L levels sustain the investigation of relationship between LECAM polymorphisms and the disease risk. The aim of the study was to investigate a possible relationship between Pro213Ser polymorphism in LECAM-1 gene and advanced stages of diabetic nephropathy in type 1 diabetes. We enrolled unrelated Caucasian patients with type 1 diabetes mellitus, fall into control group – diabetic patients with duration of disease over 20 years without microalbuminuria (n = 83 and nephropathy group – patients with overt nephropathy or end stage renal disease – ESRD (n = 121. Pro213Ser polymorphism genotyping was achieved using PCR-RFLP technique. Genotype spread analysis indicates that ProSer and SerSer are more frequent in the nephropathy group (ProSer = 41.95% and SerSer = 6.02 %, compared with the control group (ProSer = 20.53% and SerSer = 1.73%. The corrected OR's due to the small number of patients pointed the possibility that the 213Ser is the risk allele (ORSer = 1.634; p=0.04, and 213Pro is the protective one (ORPro = 0.602; p=0.04 regarding diabetic nephropathy. Thus our results suggest a possible association between the P213S polymorphism and advanced stages of diabetic nephropathy in type 1 diabetic patients. Additional researches are required in order to acknowledge the mentioned results and to clarify the mechanism by which this polymorphism intervenes in the disease pathogenesis.

  13. Somatostatin-receptor-targeted {alpha}-emitting {sup 213}Bi is therapeutically more effective than {beta}{sup -}-emitting {sup 177}Lu in human pancreatic adenocarcinoma cells

    Nayak, Tapan K. [Radiopharmaceutical Sciences Program, College of Pharmacy, University of New Mexico, Albuquerque, NM 87131-0001 (United States); Department of Cell Biology and Physiology, School of Medicine, University of New Mexico, Albuquerque, NM 87131 (United States); Norenberg, Jeffrey P. [Radiopharmaceutical Sciences Program, College of Pharmacy, University of New Mexico, Albuquerque, NM 87131-0001 (United States)]. E-mail: jpnoren@unm.edu; Anderson, Tamara L. [Radiopharmaceutical Sciences Program, College of Pharmacy, University of New Mexico, Albuquerque, NM 87131-0001 (United States); Prossnitz, Eric R. [Department of Cell Biology and Physiology, School of Medicine, University of New Mexico, Albuquerque, NM 87131 (United States); Stabin, Michael G. [Department of Radiology and Radiological Sciences, Vanderbilt University, Nashville, TN 37232 (United States); Atcher, Robert W. [Radiopharmaceutical Sciences Program, College of Pharmacy, University of New Mexico, Albuquerque, NM 87131-0001 (United States); Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545 (United States)

    2007-02-15

    Introduction: Advance clinical cancer therapy studies of patients treated with somatostatin receptor (sstr)-targeted [DOTA{sup 0}-Tyr{sup 3}]octreotide (DOTATOC) labeled with low-linear-energy-transfer (LET) {beta}{sup -}-emitters have shown overall response rates in the range of 15-33%. In order to improve outcomes, we sought to compare the therapeutic effectiveness of sstr-targeted high-LET {alpha}-emitting {sup 213}Bi to that of low-LET {beta}{sup -}-emitting {sup 177}Lu by determining relative biological effectiveness (RBE) using the external {gamma}-beam of {sup 137}Cs as reference radiation. Methods: Sstr-expressing human pancreatic adenocarcinoma Capan-2 cells and A549 control cells were used for this study. The effects of different radiation doses of {sup 213}Bi and {sup 177}Lu labeled to 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid and sstr-targeted DOTATOC were investigated with a clonogenic cell survival assay. Apoptosis was measured using the Cell Death Detection ELISA{sup PLUS} 10x kit. Results: Using equimolar DOTATOC treatment with concurrent irradiation with a {sup 137}Cs source as reference radiation, the calculated RBE of [{sup 213}Bi]DOTATOC was 3.4, as compared to 1.0 for [{sup 177}Lu]DOTATOC. As measured in terms of absorbance units, [{sup 213}Bi]DOTATOC caused a 2.3-fold-greater release of apoptosis-specific mononucleosomes and oligonucleosomes than [{sup 177}Lu]DOTATOC at the final treatment time of 96 h (P<.001) in sstr-expressing Capan-2 cells. Conclusions: In conclusion, at the same absorbed dose, [{sup 213}Bi]DOTATOC is therapeutically more effective in decreasing survival than is [{sup 177}Lu]DOTATOC in human pancreatic adenocarcinoma cells due to its comparatively higher RBE.

  14. Comparative analysis of CD138 antigen targeting for the treatment of multiple myeloma with bismuth-213 and Melphalan chemotherapy

    Full text of publication follows. Aim: multiple myeloma (MM) is a B-cell malignancy of terminally differentiated plasma cells within the bone marrow, with the presence of a monoclonal immunoglobulin in serum and/or urine and development of osteolytic bone lesions in human. Despite intense research to develop new treatments, cure is almost never achieved. Alpha-radioimmunotherapy (RIT) has been shown to be effective in vivo in a multiple myeloma model and seems particularly suited for disseminated tumour cells or small clusters of tumour cells. CD138 (Syndecan-1) is found mainly in epithelial cells, but has been shown to be expressed by most myeloma cells, both in human and in mouse. In order to define where alpha RIT stands in MM treatment, the aim of this study was to compare Melphalan, MM standard treatment, with alpha RIT using a bismuth-213-labelled anti-mouse CD138 rat antibody in a syngeneic mouse MM model. Material and Methods: C57BL/KaLwRij mice were grafted with 106 5T33 cells (murine myeloma cells). Luciferase transfected 5T33 were used for in vivo localization of the cells during the course of disease. The first step of the study was to assess the dose-response of Melphalan (100, 200 et 300 μg/mouse), 21 days after engraftment. The second step consisted in therapeutic association: Melphalan followed by RIT at d22 et d25 after engraftment. Toxicity (animal weight, blood cell counts) and treatment efficacy were studied in animals receiving no treatment, injected with Melphalan alone (200 μg), RIT alone at d22 and d25 (3.7 MBq of 213Bi-anti-CD138) and Melphalan combined with alpha RIT. Results: fifty percent of untreated mice died by d64 after MM engraftment. In mice treated with Melphalan alone, only the 200 μg dose improved median survival. No animal was cured after Melphalan treatment whereas 60% of the mice survived with RIT alone at d22 after tumour engraftment. However, the therapeutic window seems to be narrow, indeed no effect was observed with

  15. Comparative analysis of multiple myeloma treatment by CD138 antigen targeting with bismuth-213 and Melphalan chemotherapy

    Introduction: Multiple myeloma (MM) is a B-cell malignancy of terminally differentiated plasma cells within the bone marrow. Despite intense research to develop new treatments, cure is almost never achieved. Alpha-radioimmunotherapy (RIT) has been shown to be effective in vivo in a MM model. In order to define where alpha-RIT stands in MM treatment, the aim of this study was to compare Melphalan, MM standard treatment, with alpha-RIT using a [213Bi]-anti-mCD138 antibody in a syngeneic MM mouse model. Methods: C57BL/KaLwRij mice were grafted with 1 × 106 5T33 murine MM cells. Luciferase transfected 5T33 cells were used for in vivo localization. The first step of the study was to assess the dose-response of Melphalan 21 days after engraftment. The second step consisted in therapeutic combination: Melphalan followed by RIT at day 22 or day 25 after engraftment. Toxicity (animal weight, blood cell counts) and treatment efficacy were studied in animals receiving no treatment, injected with Melphalan alone, RIT alone at day 22 or day 25 (3.7 MBq of [213Bi]-anti-CD138) and Melphalan combined with alpha-RIT. Results: Fifty percent of untreated mice died by day 63 after MM engraftment. In mice treated with Melphalan alone, only the 200 μg dose improved median survival. No animal was cured after Melphalan treatment whereas 60% of the mice survived with RIT alone at day 22 after tumor engraftment with only slight and reversible hematological radiotoxicity. No therapeutic effect was observed with alpha-RIT 25 days after engraftment. Melphalan and alpha-RIT combination does not improve overall survival compared to RIT alone, and results in increased leukocyte and red blood cell toxicity. Conclusions: Alpha-RIT seems to be a good alternative to Melphalan. Association of these two treatments provides no benefit. The perspectives of this work would be to evaluate RIT impact on the regimens incorporating the novel agents bortezomide, thalidomide and lenalidomide

  16. Study on the scenic design of tunnel portals on Langchuan road of the 213th national highway%国道213线郎川公路隧道洞口景观设计方法探讨

    韩斌; 潘荣伟

    2006-01-01

    隧道洞口是隧道的门户,在隧道工程中有着很重要的地位.随着社会对土木工程结构物(隧道、桥梁等)的要求越来越高,使得景观设计成为隧道结构物设计的重要内容之一.目前,景观设计的概念虽已引入到隧道洞口设计当中,但有些隧道洞门仍采用结构单一,形式呆板的洞门.本文以国道213线郎川公路的四座隧道为例,探讨隧道洞口景观设计的方法,以期能为隧道洞口景观设计提供参考.

  17. Measurements of high energy neutrons penetrated through iron shields using the Self-TOF detector and an NE213 organic liquid scintillator

    Sasaki, M; Nunomiya, T; Nakamura, T; Fukumura, A; Takada, M

    2002-01-01

    Neutron energy spectra penetrated through iron shields were measured using the Self-TOF detector and an NE213 organic liquid scintillator which have been newly developed by our group at the Heavy-Ion Medical Accelerator in Chiba (HIMAC) of National Institute of Radiological Sciences (NIRS), Japan. Neutrons were generated by bombarding 400 MeV/nucleon C ion on a thick (stopping-length) copper target. The neutron spectra in the energy range from 20 to 800 MeV were obtained through the FORIST unfolding code with their response functions and compared with the MCNPX calculations combined with the LA150 cross section library. The neutron fluence measured by the NE213 detector was simulated by the track length estimator in the MCNPX, and evaluated the contribution of the room-scattered neutrons. The calculations are in fairly good agreement with the measurements. Neutron fluence attenuation lengths were obtained from the experimental results and the calculation.

  18. Input model of a VVER 440/213 fuel assembly and CFD calculations by the FLUENT code

    The preparation of the final version of the computation network of a VVER 440/213 fuel assembly by the GAMBIT code is described. Qualified estimates of the size of the networks (numbers of cells) are made especially in dependence on the network density in the transverse section. Some sensitivity analyses performed on smaller geometries, devoted to the effect of computation network density on turbulence modelling and to the effect of the limiting layer thickness on the temperature and flow rate fields are described. Of importance was the analysis of spacer grid replacement by means of the porous media function with regard to savings in the number of computational cells. The CFD calculations in the FLUENT code, performed first for smaller test problems and subsequently for large problems describing 1/6 to 1/2 of the fuel assembly, are described. Analyzed are the coolant distribution within the assembly after passing the bottom supporting plate and the effect of the spacer grids on coolant distribution and on heat transfer

  19. Clinical Observation on Termination of Early Pregnancy of 213 Cases after Caesarian Section with Repeated Use of Mifepristone and Misoprostol

    高佩佩; 汪平

    1999-01-01

    Objective To investigate the efficacy and safety in women after caesarian section for termination of early pregnancies by treatment, or repeated treatment with mifepristone and misoprostot.Subjects and Methods A total of 213 pregnant women with amenorrhea of 34-69d after caesarian section who asked for medical abortion were recruited,including 63 cases undergoing their second medical abortion.A total amount of mi feprisstone of 150 mg given in separate doses(25 mg×4 and 50 mg at the first time)was administered orally within 3d, followed by misoprostot of 0.6 mg orally in the morning of d 3.Results The complete abortion rate was 92.5%,incomplete abortion was 4.7% and failure was 2.8%.Conclusion The sequential use of mifepristone and misoprostol could be successfully and repeatedly used for induced abortion in those women with a caesarian section histo-ry.Its efficacy was similar to that for ordinary population.Its safety and effec-tiveness were satisfactory.

  20. [Determination of 213 pesticide residues in milk and milk power by gas chromatography-triple quadrupole tandem mass spectrometry].

    Wang, Jing; Ai, Lianfeng; Ma, Yusong; Zhang, Haichao; Li, Wei; Yu, Meng

    2015-11-01

    On the basis of the optimization of solid phase extraction adsorbent, eluting solvent types and amounts, a gas chromatography-triple quadrupole tandem mass spectrometry (GC-MS/MS) method was established for the determination of 213 pesticide residues in milk and milk power. The samples were extracted by acetonitrile, cleaned-up with an ENVI-Carb/NH2 solid-phase extractant, and determined by GC-MS/MS using external standard method. The linear ranges were from 10 to 1 000 μg/L for 197 pesticides, from 50 to 1 000 μg/L for the other 16 pesticides with the correlation coefficients higher than 0.99. The limits of detection (LODs, S/N = 3) varied over the range of 0.03 to 7.59 μg/kg, and limits of quantification (LOQs, S/N = 10) ranged from 0.10 to 21.94 μg/kg. The average recoveries in different matrices were in the range of 66.9% - 120.1% with the relative standard deviations (RSDs) of 1.23% - 17.6%. This method is simple, rapid, sensitive and reliable for meeting the requirements for the simultaneous identification and quantification of the multi-residues in milk and milk power. PMID:26939364

  1. Peroxidase-catalyzed metabolism of etoposide (VP-16-213) and covalent binding of reactive intermediates to cellular macromolecules

    The horseradish peroxidase- and prostaglandin synthetase-catalyzed oxidative metabolism of the highly active anticancer drug, etoposide (VP-16-213), has been studied in vitro. This oxidation of VP-16 resulted in the formation of VP-16 quinone, an aromatic VP-16 derivative and the corresponding aromatic VP-16 quinone. This oxidative metabolism of VP-16 also resulted in the formation of reactive species that covalently bound to exogenously added DNA and heat-inactivated microsomal proteins. The peroxidase-catalyzed binding was time dependent and required the presence of cofactors (hydrogen peroxide or arachidonic acid). The prostaglandin synthetase/arachidonic acid-catalyzed metabolism and binding of VP-16 were inhibited by indomethacin, an inhibitor of the cyclooxygenase, and were shown to involve the peroxidative arm of prostaglandin synthetase. Our studies show that the protein covalent binding species were formed as a result of O-demethylation of the drug as shown by the loss of specifically labeled (O-14CH3) radioactivity from O-methoxy group and by incubating proteins with VP-16 quinones. In contrast, the covalent binding intermediates for DNA appeared to be different and VP-16-derived quinone methides are suggested as DNA binding species. Co-oxidation of VP-16 and the related drug, VM-26, during prostaglandin biosynthesis may be an important pathway for the metabolism of these agents and may play a role in their cytotoxic properties

  2. Human protein kinase C lota gene (PRKC1) is closely linked to the BTK gene in Xq21.3

    Mazzarella, R.; Jones, C.; Schlessinger, D. [Washington Univ. School of Medicine, St. Louis, MO (United States)] [and others

    1995-04-10

    The human X chromosome contains many disease loci, but only a small number of X-linked genes have been cloned and characterized. One approach to finding genes in genomic DNA uses partial sequencing of random cDNAs to develop {open_quotes}expressed sequence tags{close_quotes} (ESTs). Many authors have recently reported chromosomal localization of such ESTs using hybrid panels. Twenty ESTs specific for the X chromosome have been localized to defined regions with somatic cell hybrids, and 12 of them have been physically linked to markers that detect polymorphisms. One of these ESTs, EST02087, was physically linked in a 650-kb contig to the GLA ({alpha}-galactosidase) gene involved in Fabry disease. A comparison of this contig with a 7.5-Mb YAC contig indicated that this gene is also within 250 kb of the src-like protein-tyrosine kinase BTK (X-linked agammaglobulinemia protein-tyrosine kinase) gene in Xq21.3. 14 refs., 1 fig.

  3. Split hand/split foot malformation, deafness, and mental retardation with a complex cytogenetic rearrangement involving 7q21.3.

    Ignatius, J.; Knuutila, S; Scherer, S W; Trask, B; Kere, J

    1996-01-01

    Split hand/split foot malformation (SHSF) has been described in several patients associated with cytogenetically visible rearrangements involving chromosome 7q. Characterisation of these patients has led to localisation of an autosomal dominant form of SHSF to 7q21-22; the locus has been designated SHFM1. We describe a patient with a complex, apparently balanced cytogenetic rearrangement, including a translocation breakpoint at 7q21.3 near the DSS1 gene. In addition to ectrodactyly of all fou...

  4. Replication of the association of chromosomal region 9p21.3 with generalized aggressive periodontitis (gAgP using an independent case-control cohort

    Ernst Florian D

    2010-08-01

    Full Text Available Abstract Background The human chromosomal region 9p21.3 has been shown to be strongly associated with Coronary Heart Disease (CHD in several Genome-wide Association Studies (GWAS. Recently, this region has also been shown to be associated with Aggressive Periodontitis (AgP, strengthening the hypothesis that the established epidemiological association between periodontitis and CHD is caused by a shared genetic background, in addition to common environmental and behavioural risk factors. However, the size of the analyzed cohorts in this primary analysis was small compared to other association studies on complex diseases. Using our own AgP cohort, we attempted to confirm the described associations for the chromosomal region 9p21.3. Methods We analyzed our cohort consisting of patients suffering from the most severe form of AgP, generalized AgP (gAgP (n = 130 and appropriate periodontally healthy control individuals (n = 339 by genotyping four tagging SNPs (rs2891168, rs1333042, rs1333048 and rs496892, located in the chromosomal region 9p21.3, that have been associated with AgP. Results The results confirmed significant associations between three of the four SNPs and gAgP. The combination of our results with those from the study which described this association for the first time in a meta-analysis of the four tagging SNPs produced clearly lower p-values compared with the results of each individual study. According to these results, the most plausible genetic model for the association of all four tested SNPs with gAgP seems to be the multiplicative one. Conclusion We positively replicated the finding of an association between the chromosomal region 9p21.3 and gAgP. This result strengthens support for the hypothesis that shared susceptibility genes within this chromosomal locus might be involved in the pathogenesis of both CHD and gAgP.

  5. Preparation of 2'-13C-L-Histidine Starting from 13C-Thiocyanate: Synthetic Access to Any Site-Directed Stable Isotope Enriched L-Histidine

    Sarra Talab; Kamal Khalifa Taha; Johan Lugtenburg

    2014-01-01

    1-Benzyl-2-(methylthio)-imidazole-5-ketone is obtained in a few simple steps starting from thiocyanate and glycine amide (glycin). Subsequent treatment with diethyl phosphorocyanidate and functional group manipulations gives 1-benzyl-5-chloromethyl-imidazolium chloride. This compound is converted under mild O’Donnell conditions into the corresponding L-histidine derivative. After deprotection L-histidine is obtained in good yield and 99% enantiomeric excess. 2'-13C-L-Histidine has been obtai...

  6. Non-invasive visualisation of the development of peritoneal carcinomatosis and tumour regression after 213Bi-radioimmunotherapy using bioluminescence imaging

    Non-invasive imaging of tumour development remains a challenge, especially for tumours in the intraperitoneal cavity. Therefore, the aim of this study was the visualisation of both the development of peritoneal carcinomatosis and tumour regression after radioimmunotherapy with tumour-specific 213Bi-Immunoconjugates, via in vivo bioluminescence imaging of firefly luciferase-transfected cells. Human diffuse-type gastric cancer cells expressing mutant d9-E-cadherin were stably transfected with firefly luciferase (HSC45-M2-luc). For bioluminescence imaging, nude mice were inoculated intraperitoneally with 1 x 107 HSC45-M2-luc cells. On days 4 and 8 after tumour cell inoculation, imaging was performed following D-luciferin injection using a cooled CCD camera with an image intensifier unit. For therapy, mice were injected with 2.7 MBq 213Bi-d9MAb targeting d9-E-cadherin on day 8 after tumour cell inoculation. Bioluminescence images were taken every 4 days to monitor tumour development. After i.p. inoculation of HSC45-M2-luc cells into nude mice, development as well as localisation of peritoneal carcinomatosis could be visualised using bioluminescence imaging. Following 213Bi-d9MAb therapy on day 8 after intraperitoneal inoculation of HSC45-M2-luc cells, small tumour nodules were totally eliminated and larger nodules showed a clear reduction in size on day 12 after tumour cell inoculation. Subsequently a recurrence of tumour mass was observed, starting from the remaining tumour spots. By measuring the mean grey level intensity, tumour development over time could be demonstrated. Non-invasive bioluminescence imaging permits visualisation of the development of peritoneal carcinomatosis, localisation of tumour in the intraperitoneal cavity and evaluation of therapeutic success after 213Bi-d9MAb treatment. (orig.)

  7. Crystallization and initial crystallographic analysis of the Streptococcus parasanguinis FW213 Fap1-­NRα adhesive domain at pH 5.0

    Garnett, James A; Ramboarina, Stéphanie; Lee, Wei-chao; Tagliaferri, Camille; Wu, Wilfred; MATTHEWS, STEPHEN

    2011-01-01

    The adhesin fimbriae-associated protein 1 (Fap1) is a surface protein of Streptococcus parasanguinis FW213 and plays a major role in the formation of dental plaque in humans. Here, the adhesive domain Fap1-NRα, which is activated by acidic pH, has been crystallized at pH 5.0 and diffraction data have been collected to 3.0 Å resolution.

  8. Synthese, elektrochemische Eigenschaften und Struktur von Metallkomplexen mit 2,1,3-Benzochalkogenadiazol-Liganden und spektroelektrochemische Untersuchungen ausgewählter Zweikernkomplexe

    Plebst, Sebastian

    2015-01-01

    Kapitel 2 und 3 dieser Dissertation beschäftigen sich mit den Koordinationseigenschaften von 2,1,3 Benzochalkogenadiazol Liganden (BCD) (Kap. 2) in Verbindung mit Wolfram und Rheniumkomplexfragmenten sowie mit dem Di Benzochalkogenadiazol System des Bis ([1,2,5]thiadiazolo[2,3 c:5,6c‘]) 1,4 benzochinon (BTDB) und seinen Rheniumcarbonylkomplexen (Kap. 3). Neben der strukturellen und rechnerischen Analyse der Komplexe 1 und 2 konnten erstmalig spektroelektrochemische Messungen im UV/Vis Be...

  9. Single-dose anti-CD138 radioimmunotherapy: bismuth-213 is more efficient than lutetium-177 for treatment of multiple myeloma in a preclinical model

    Fichou, Nolwenn; Gouard, Sébastien; Maurel, Catherine; Barbet, Jacques; Ferrer, Ludovic; Morgenstern, Alfred; Bruchertseifer, Frank; Faivre-Chauvet, Alain; Bigot-Corbel, Edith; Davodeau, François; Gaschet, Joëlle; Chérel, Michel

    2015-01-01

    Objectives Radioimmunotherapy (RIT) has emerged as a potential treatment option for multiple myeloma (MM). In humans, a dosimetry study recently showed the relevance of RIT using an antibody targeting the CD138 antigen. The therapeutic efficacy of RIT using an anti-CD138 antibody coupled to 213Bi, an α-emitter, was also demonstrated in a preclinical MM model. Since then, RIT with β-emitters has shown efficacy in treating hematologic cancer. In this paper, we investigate the therapeutic eff...

  10. Preclinical evaluation of NETA-based bifunctional ligand for radioimmunotherapy applications using 212Bi and 213Bi: Radiolabeling, serum stability, and biodistribution and tumor uptake studies

    Introduction: Despite the great potential of targeted α-radioimmunotherapy (RIT) as demonstrated by pre-clinical and clinical trials, limited progress has been made on the improvement of chelation chemistry for 212Bi and 213Bi. A new bifunctional ligand 3p-C-NETA was evaluated for targeted α RIT using 212Bi and 213Bi. Methods: Radiolabeling of 3p-C-NETA with 205/6Bi, a surrogate of 212Bi and 213Bi, was evaluated at pH 5.5 and room temperature. In vitro stability of the 205/6Bi-3p-C-NETA-trastuzumab conjugate was evaluated using human serum (pH 7, 37 °C). Immunoreactivity and specific activity of the 205/6Bi-3p-C-NETA-trastuzumab conjugate were measured. An in vivo biodistribution study was performed to evaluate the in vivo stability and tumor targeting properties of the 205/6Bi-3p-C-NETA-trastuzumab conjugate in athymic mice bearing subcutaneous LS174T tumor xenografts. Result: The 3p-C-NETA-trastuzumab conjugate was extremely rapid in complexing with 205/6Bi, and the corresponding 205/6Bi-3p-C-NETA-trastuzumab was stable in human serum. 205/6Bi-3p-C-NETA-trastuzumab was prepared with a high specific activity and retained immunoreactivity. 205/6Bi-3p-C-NETA-trastuzumab conjugate displayed excellent in vivo stability and targeting as evidenced by low normal organ and high tumor uptake. Conclusion: The results of the in vitro and in vivo studies indicate that 3p-C-NETA is a promising chelator for RIT applications using 212Bi and 213Bi. Further detailed in vivo evaluations of 3p-C-NETA for targeted α RIT are warranted

  11. Experience from operation of WWER-440 model 213 nuclear power plants. Reference plant: Bohunice V2 (Slovakia). Report of the IAEA technical co-operation project RER/9/004 on evaluation of safety aspects of WWER-440 model 213 nuclear power plants

    This TECDOC provides a comprehensive review of the operational experience with WWER-440/213 plants. It is hoped that it will be useful to anyone working in the field of WWER safety, and in particular to experts planning, executing or reviewing studies related to the subject. Refs, figs and tabs

  12. Experimental design verification of WWER-440 model 213 nuclear power plants. Reference plant: Bohunice V2 (Slovakia). Report of the IAEA technical co-operation project RER/9/004 on evaluation of safety aspects of WWER-440 model 213 nuclear power plants

    This publication addresses the experimental research supporting the design of WWER-440 model 213 plants. it is hoped that the material presented will be useful for experts working in the field of WWER safety, and in particular to those planning, executing or reviewing studies related to the subject. Refs, figs and tabs

  13. Methemoglobin-producing effect and its role in the mechanism of action of some radioprotective agents in the benzo-2,1,3-thiadiazole class. [Mice, dogs, gamma radiation

    Vladimirov, V.G.; Chigareva, N.G.; Belen' kaya, I.A.; Strel' nikov, Yu.E.

    1977-01-01

    A study was made of some derivatives of benzo-2,1,3-thiadiazole with regard to their capacity to form methemoglobin in blood. It was demonstrated that, 30 min after intraperitoneal administration of radioprotective doses of such agents to mice, the blood methemoglobin level does not exceed 5 percent. With administration to mice of 4-aminobenzo-2,1,3-thiadiazole in a toxic dose 14 percent methemoglobin is produced in blood. In dogs, almost 50 percent methemoglobin is formed in blood after oral intake of 4-aminobenzo-2,1,3-thiadiazole; this is a level that can provide for the radioprotective action of this compound. Using agents that prevent oxidation of hemoglobin to methemoglobin (2-methoxy-9-phenazine sulfate and isonicotinic acid hydrazide), it was demonstrated that the methemoglobin formed in mouse blood does not play a leading role in manifestation of the radioprotective action and toxic properties of 4-aminobenzo-2,1,3-thiadiazole.

  14. Neutron-Gamma Pulse Shape Discrimination with a NE-213 Liquid Scintillator by Using Digital Signal Processing Combined with Similarity Method

    Mardiyanto

    2008-07-01

    Full Text Available Neutron-Gamma Pulse Shape Discrimination with a NE-213 Liquid Scintillator by Using Digital Signal Processing Combined with Similarity Method. Measurement of mixed neutron-gamma radiation is difficult because a neuclear detector is usually sensitive to both radiations. A new attempt of neutron-gamma pulse shape discrimination for a NE-213 liquid scintillator is presented by using digital signal processing combined with an off-line similarity method. The output pulse shapes are digitized with a high speed digital oscilloscope. The n-γ discrimination is done by calculating the index of each pulse shape, which is determined by the similarity method, and then fusing it with its corresponding pulse height. Preliminary results demonstrate good separation of neutron and gamma-ray signals from a NE-213 scintillator with a simple digital system. The results were better than those with a conventional rise time method. Figure of Merit is used to determine the quality of discrimination. The figure of merit of the discrimination using digital signal processing combined with of line similarity method are 1.9; 1.7; 1.1; 1.1; and 0.8 ; on the other hand by using conventional method the rise time are 0.9; 0.9; 0.9; 0.7; and 0.4 for the equivalent electron energy of 800 ; 278 ; 139 ; 69 ; and 30 keV

  15. MR-guided radiofrequency ablation using a wide-bore 1.5-T MR system: clinical results of 213 treated liver lesions

    To evaluate the technical effectiveness, technical success and patient safety of MR-guided radiofrequency (RF) ablation of liver malignancies using a wide-bore 1.5-T MR system. In 110 patients, 56 primary liver lesions and 157 liver metastases were treated in 157 sessions using percutaneous RF ablation. Mean lesion diameter was 20 mm (range 4-54 mm). All planning, procedural and post-interventional control MR investigations were carried out using a wide-bore 1.5-T MR system. Technical success was assessed by a contrast-enhanced MR liver examination immediately after the intervention. Technique effectiveness was assessed by dynamic hepatic MR study 1 month post ablation; mean follow-up period was 24.2 months (range 5-44). Technical success and technique effectiveness were achieved in 210/213 lesions (98.6 %). In 18/210 lesions (8.6 %), local tumour progression occurred 4-28 months after therapy. Seven of these 18 lesions were treated in a second session achieving complete ablation, 6 other lesions were referred to surgery. Overall RF effectiveness rate was 199/213 (93.4 %); overall therapy success (including surgery) was 205/213 (96.2 %). Two major complications (1.3 %) (bleeding and infected biloma) and 14 (8.9 %) minor complications occurred subsequent to 157 interventions. Wide-bore MR-guided RF ablation is a safe and effective treatment option for liver lesions. (orig.)

  16. Chemoimmunotherapy of small cell bronchogenic carcinoma with VP-16-213, ifosfamide, vincristine, adriamycin, and Corynebacterium parvum

    Thirty-five consecutive patients with small cell bronchogenic carcinoma (SCBC) received chemoimmunotherapy with VP-16-213, Ifosfamide, vincristine, Adriamycin, and Corynebacterium parvum. Of 33 evaluable patients, 26 (79%) responded with complete (55%) or partial (24%) remissions. Complete remissions were more common among patients with limited disease (11/14 patients, 79%) compared with those with extensive disease (7/19 patients, 37%) and among patients (11/14 patients, 79%) compared with those with extensive disease (7/19 patients, 37%) and among patients who were ambulatory prior to therapy (16/25 patients, 64%) compared with those who were nonambulatory (2/8 patients, 25%). Myelosuppression consisted primarily of neutropenia. Eight percent of the treatment courses in 29% of the patients were associated with hematuria and/or documented episodes of infection during neutropenia. There were three deaths possibly related to treatment, in two of which there was no evidence of disease at post-mortem examination. Six patients relapsed in the central nervous system (CNS). In four instances, CNS relapse was the only site of tumor progression. Central nervous system relapse was more common among evaluable patients who did not receive prophylactic brain irradiation (5/17 patients, 29%, vs. 1/15 patients, 7%; P . 0.23). The median survival duration for all patients was 63 weeks, being slightly longer for patients with limited disease than for those with extensive disease (70.9 weeks vs. 56 weeks; P . 0.18). This was also true for patients who achieved complete rather than partial remissions (71 weeks vs. 50 weeks; P . 0.09). Patients receiving prophylactic brain irradiation experienced longer survival

  17. A prospective multicentre study of mycophenolate mofetil combined with prednisolone as induction therapy in 213 patients with active lupus nephritis.

    F, Lu; Y, Tu; X, Peng; L, Wang; H, Wang; Z, Sun; H, Zheng; Z, Hu

    2008-07-01

    Mycophenolate mofetil (MMF) with prednisolone has been associated with high remission rates when used as induction treatment for lupus nephritis. This prospective, multicentre, cohort study investigates the efficacy and safety of this regimen over 24 weeks in 213 Chinese patients with active lupus nephritis (Classes III, IV, V or combination). Baseline activity index (AI) was 6.91+/-3.33 and chronicity index (CI) was 1.9+/-1.2. The remission rate was 82.6% at 24 weeks (complete remission, 34.3%; partial remission, 48.4%). There were significant (P<0.01) improvements in kidney function shown by reductions in proteinuria, serum albumin, serum creatinine and creatinine clearance, as well as in systemic lupus erythematosus disease activity index (SLEDAI) scores. Independent risk factors influencing remission were pathological classification (including Class V and III or Class V and IV nephritis) and elevated serum creatinine at baseline (OR 2.967, 95% CI: 1.479-6.332, P=0.001 and OR 1.007, 95% CI: 1.002-1.011, P=0.001, respectively). Patients with concomitant membranous features on biopsy had a lower remission rate than those with Class III and IV nephritis (66.7% vs 87.3%, P=0.002). Renal biopsy was repeated in 25 patients following treatment. There was a transition to less severe pathological morphologies in majority of subjects. Infections were monitored throughout treatment: eight patients (3.8%) experienced bacterial infections, whereas herpes zoster occurred in seven patients. Nine patients (4.2%) suffered from gastrointestinal upset, which resolved without discontinuation of MMF. One patient became leucopenic, whereas another died from active disease unrelated to kidney symptoms. MMF combined with prednisolone is an effective and well-tolerated induction treatment for patients with active lupus nephritis and for controlling SLE systemic activity. PMID:18625634

  18. Downregulation of Securin by the variant RNF213 R4810K (rs112735431, G>A) reduces angiogenic activity of induced pluripotent stem cell-derived vascular endothelial cells from moyamoya patients

    Hitomi, Toshiaki [Department of Health and Environmental Sciences, Kyoto University, Kyoto (Japan); Habu, Toshiyuki [Radiation Biology Center, Kyoto University, Kyoto (Japan); Kobayashi, Hatasu; Okuda, Hiroko; Harada, Kouji H. [Department of Health and Environmental Sciences, Kyoto University, Kyoto (Japan); Osafune, Kenji [Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto (Japan); Taura, Daisuke; Sone, Masakatsu [Department of Medicine and Clinical Science, Kyoto University, Kyoto (Japan); Asaka, Isao; Ameku, Tomonaga; Watanabe, Akira; Kasahara, Tomoko; Sudo, Tomomi; Shiota, Fumihiko [Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto (Japan); Hashikata, Hirokuni; Takagi, Yasushi [Department of Neurosurgery, Kyoto University,Kyoto (Japan); Morito, Daisuke [Faculty of Life Sciences, Kyoto Sangyo University, Kyoto (Japan); Miyamoto, Susumu [Department of Neurosurgery, Kyoto University,Kyoto (Japan); Nakao, Kazuwa [Department of Medicine and Clinical Science, Kyoto University, Kyoto (Japan); Koizumi, Akio, E-mail: koizumi.akio.5v@kyoto-u.ac.jp [Department of Health and Environmental Sciences, Kyoto University, Kyoto (Japan)

    2013-08-16

    Highlights: •Angiogenic activities were reduced in iPSECs from MMD patients. •Many mitosis-regulated genes were downregulated in iPSECs from MMD patients. •RNF213 R4810K downregulated Securin and inhibited angiogenic activity. •Securin suppression by siRNA reduced angiogenic activities of iPSECs and HUVECs. -- Abstract: Moyamoya disease (MMD) is a cerebrovascular disease characterized by occlusive lesions in the circle of Willis. The RNF213 R4810K polymorphism increases susceptibility to MMD. Induced pluripotent stem cells (iPSCs) were established from unaffected fibroblast donors with wild-type RNF213 alleles, and from carriers/patients with one or two RNF213 R4810K alleles. Angiogenic activities of iPSC-derived vascular endothelial cells (iPSECs) from patients and carriers were lower (49.0 ± 19.4%) than from wild-type subjects (p < 0.01). Gene expression profiles in iPSECs showed that Securin was down-regulated (p < 0.01) in carriers and patients. Overexpression of RNF213 R4810K downregulated Securin, inhibited angiogenic activity (36.0 ± 16.9%) and proliferation of humanumbilical vein endothelial cells (HUVECs) while overexpression of RNF213 wild type did not. Securin expression was downregulated using RNA interference techniques, which reduced the level of tube formation in iPSECs and HUVECs without inhibition of proliferation. RNF213 R4810K reduced angiogenic activities of iPSECs from patients with MMD, suggesting that it is a promising in vitro model for MMD.

  19. Downregulation of Securin by the variant RNF213 R4810K (rs112735431, G>A) reduces angiogenic activity of induced pluripotent stem cell-derived vascular endothelial cells from moyamoya patients

    Highlights: •Angiogenic activities were reduced in iPSECs from MMD patients. •Many mitosis-regulated genes were downregulated in iPSECs from MMD patients. •RNF213 R4810K downregulated Securin and inhibited angiogenic activity. •Securin suppression by siRNA reduced angiogenic activities of iPSECs and HUVECs. -- Abstract: Moyamoya disease (MMD) is a cerebrovascular disease characterized by occlusive lesions in the circle of Willis. The RNF213 R4810K polymorphism increases susceptibility to MMD. Induced pluripotent stem cells (iPSCs) were established from unaffected fibroblast donors with wild-type RNF213 alleles, and from carriers/patients with one or two RNF213 R4810K alleles. Angiogenic activities of iPSC-derived vascular endothelial cells (iPSECs) from patients and carriers were lower (49.0 ± 19.4%) than from wild-type subjects (p < 0.01). Gene expression profiles in iPSECs showed that Securin was down-regulated (p < 0.01) in carriers and patients. Overexpression of RNF213 R4810K downregulated Securin, inhibited angiogenic activity (36.0 ± 16.9%) and proliferation of humanumbilical vein endothelial cells (HUVECs) while overexpression of RNF213 wild type did not. Securin expression was downregulated using RNA interference techniques, which reduced the level of tube formation in iPSECs and HUVECs without inhibition of proliferation. RNF213 R4810K reduced angiogenic activities of iPSECs from patients with MMD, suggesting that it is a promising in vitro model for MMD

  20. PSL(2,13)的最小级连通3度弧传递陪集图表示%The graphical representations of connected cubic arc-transitive coset graphs of minimum degree on PSL(2,13

    汤利荣

    2011-01-01

    通过寻找给定群G的图表示,对PSL(2,13)的连通3度弧传递陪集图表示进行研究,得到了如下结果:PSL(2,13)的最小级连通3度弧传递陪集图表示的级是182.并且给出了该陪集图表示的例子.%This paper studies the graphical representations of connected cubic arc-transitive coset graphs onPSL(2, 13) based on the graphical representations of given group G. The following result is proved. PSL(2, 13)has the minimum degree 182 of connected cubic arc-transitive coset graphical representations. The example ofthe coset graphical representations is given.

  1. Diazidobis[4,4,5,5-tetramethyl-2-(1,3-thiazol-2-yl-2-imidazoline-1-oxyl-3-oxide-κ2O,N]manganese(II

    Kai Jiang

    2009-02-01

    Full Text Available In the crystal structure of the title compound, [Mn(N32(C10H14N3O2S2], the Mn(II atom exhibits a roughly octahedral coordination geometry. The Mn(II atom lies on an inversion centre, thus the asymmetric unit comprises one half-molecule. The metal center is six-coordinated by two azide anions and by two chelating 4,4,5,5-tetramethyl-2-(1,3-thiazol-2-yl-2-imidazoline-1-oxyl-3-oxide nitronyl nitroxide radical ligands, leading to two six-membered chelate rings.

  2. Inhibition of lung cancer cell growth and induction of apoptosis after reexpression of 3p21.3 candidate tumor suppressor gene SEMA3B

    Tomizawa, Yoshio; Sekido, Yoshitaka; Kondo, Masashi; Gao, Boning; Yokota, Jun; Roche, Joëlle; Drabkin, Harry; Lerman, Michael I; Gazdar, Adi F.; Minna, John D.

    2001-01-01

    Semaphorins SEMA3B and its homologue SEMA3F are 3p21.3 candidate tumor suppressor genes (TSGs), the expression of which is frequently lost in lung cancers. To test the TSG candidacy of SEMA3B and SEMA3F, we transfected them into lung cancer NCI-H1299 cells, which do not express either gene. Colony formation of H1299 cells was reduced 90% after transfection with wild-type SEMA3B compared with the control vector. By contrast, only 30–40% reduction in colony formation was seen after the transfec...

  3. Carbon, Nitrogen, and Chalcogen Substitution Effects on 2,1,3-Benzothiadiazole Derivative: Theoretical Investigations of Electronic,Optical, and Charge Transport Properties

    Bo Hu; Chan Yao; Qing-wei Wang; Hao Zhang; Jian-kang Yu

    2012-01-01

    A series of CH2,NH,O,and Se substituted 2,1,3-benzothiadiazole derivatives have been designed and investigated computationally to elucidate their potential as organic light-emitting materials for organic light-emitting diodes.Both ab initio Hartree-Fock and hybrid density functional methods are used.It is found that adjusting the central aromatic ring by replacing S by CH2,NH,O,and Se makes it possible to fine-tune the electronic,optical,and charge transport properties of the pristine molecule.

  4. Adaptation of the severe accident codes to VVER-440/V213 (V230) reactor unit, their comparison and utilisation of the results

    This paper presents an application and comparison of the computer codes, devoted for severe accident analysis of PWR up to source term evaluation, to a VVER-440/V213 and V230 NPP. The basic results of selected sequences are described and some physical parameters predicted by different codes are compared. The comparison is deliberated mainly on the timing of main primary circuit events and fission products behaviour up to source term evaluation. Utilisation of the results of the severe accident analysis for development of the emergency procedures for rapid assessment of barriers status and source term category is shortly described, too. (author)

  5. Identifikasi Secara Serologi Galur Virus Flu Burung Subtipe H5N1 Clade 2.1.3 dan Clade 2.3.2 pada Ayam Petelur (SEROLOGICAL IDENTIFICATION OF AVIAN INFLUENZA STRAIN VIRUS SUBTYPE H5N1 CLADE 2.1.3 AND CLADE 2.3.2 FROM LAYER

    Aprilia Kusumastuti

    2015-10-01

    Full Text Available The aim of the study was to know avian influenza (AI infection in field by using serology test in threemarketing area of AI vaccines. Haemagglutination inhibition methode was used in this test. There werefour antigen strains of AI subtype H5N1 clade 2.1.3 (AIstrainA/Chicken/West Java/PWT-WIJ/2006, AIstrain A/Chicken/Garut/BBVW-223/2007, AI strain A/Chicken/West Java-Nagrak/30/2007, and AI strainA/Chicken/Pekalongan/BBVW-208/2007 and 2 antigen strains of AI subtype H5N1 clade 2.3.2 (AI strainA/duck/Sukoharjo/BBVW-1428-9/2012 and AI strain A/duck/Sleman/BBVW-1463-10/2012 was used inthis study for HI test. The result presents that 93,33% chicken farms in three marketing area of PT. SanbioLaboratories have positive antibody titre to AI subtype H5N1 clade 2.1.3. This titre may be obtained fromAI clade 2.1.3 vaccination. From 15 samples, 92,86% are positive to AI subtype H5N1 clade 2.3.2A/duck/Sukoharjo/BBVW-1428-9/2012 and 92,31% are positive to A/duck/Sleman/BBVW-1463-10/2012 evenwithout AI clade 2.3.2 vaccination. This antibody titre may be obtained from AI clade 2.1.3 vaccine crossprotection or field infection.

  6. Conventional and Pretargeted Radioimmunotherapy Using Bismuth-213 to Target and Treat Non-Hodgkin Lymphomas Expressing CD20: A Preclinical Model toward Optimal Consolidation Therapy to Eradicate Minimal Residual Disease

    Radioimmunotherapy (RIT) with α-emitting radionuclides is an attractive approach for the treatment of minimal residual disease (MRD) because the short path lengths and high energies of α-particles produce optimal cytotoxicity at small target sites while minimizing damage to surrounding normal tissues. Pretargeted RIT (PRIT) using antibody-streptavidin (Ab-SA) constructs and radiolabeled biotin allows rapid, specific localization of radioactivity at tumor sites, making it an optimal method to target α-emitters with short half-lives, such as bismuth-213 (213Bi). Athymic mice bearing Ramos lymphoma xenografts received anti-CD20 1F5(scFv)4SA fusion protein (FP), followed by a dendrimeric clearing agent and (213Bi)DOTA-biotin. After 90 min, tumor uptake for 1F5(scFv)4SA was 16.5 ± 7.0 % injected dose per gram (ID/g) compared with 2.3 ± 0.9 % ID/g for the control FP. Mice treated with anti-CD20 PRIT and 600 (micro)Ci (213Bi)DOTA-biotin exhibited marked tumor growth delays compared to controls (mean tumor volume 0.01 ± 0.02 vs. 203.38 ± 83.03 mm3 after 19 days, respectively). The median survival for the 1F5(scFv)4SA group was 90 days compared to 23 days for the control FP (p 213Bi-labeled anti-CD20 PRIT.

  7. Crystallization and initial crystallographic analysis of the Streptococcus parasanguinis FW213 Fap1-NRα adhesive domain at pH 5.0

    The adhesin fimbriae-associated protein 1 (Fap1) is a surface protein of Streptococcus parasanguinis FW213 and plays a major role in the formation of dental plaque in humans. Here, the adhesive domain Fap1-NRα, which is activated by acidic pH, has been crystallized at pH 5.0 and diffraction data have been collected to 3.0 Å resolution. The adhesin fimbriae-associated protein 1 (Fap1) is a surface protein of Streptococcus parasanguinis FW213 and plays a major role in the formation of dental plaque in humans. Increased adherence is highly correlated to a reduction in pH and acid activation has been mapped to a subdomain: Fap1-NRα. Here, Fap1-NRα has been crystallized at pH 5.0 and diffraction data have been collected to 3.0 Å resolution. The crystals belonged to space group P41212 or P43212, with unit-cell parameters a = b = 122.0, c = 117.8 Å. It was not possible to conclusively determine the number of molecules in the asymmetric unit and heavy-atom derivatives are now being prepared

  8. Targeted alpha-radionuclide therapy of functionally critically located gliomas with {sup 213}Bi-DOTA-[Thi{sup 8},Met(O{sub 2}){sup 11}]-substance P: a pilot trial

    Cordier, D.; Merlo, A. [University Hospitals, Division of Neurosurgery, Basel (Switzerland); Forrer, F.; Good, S.; Mueller-Brand, J. [University Hospitals, Institute of Nuclear Medicine, Basel (Switzerland); Bruchertseifer, F.; Morgenstern, A.; Apostolidis, C. [Institute for Transuranium Elements, European Commission, Joint Research Centre, Karlsruhe (Germany); Maecke, H. [University Hospitals, Institute of Nuclear Medicine, Basel (Switzerland); University Hospital Basel, Nuclear Medicine and Radiological Chemistry, Basel (Switzerland); Reubi, J.C. [University of Berne, Institute of Pathology, Berne (Switzerland)

    2010-07-15

    Functionally critically located gliomas represent a challenging subgroup of intrinsic brain neoplasms. Standard therapeutic recommendations often cannot be applied, because radical treatment and preservation of neurological function are contrary goals. The successful targeting of gliomas with locally injected beta radiation-emitting {sup 90}Y-DOTAGA-substance P has been shown previously. However, in critically located tumours, the mean tissue range of 5 mm of {sup 90}Y may seriously damage adjacent brain areas. In contrast, the alpha radiation-emitting radionuclide {sup 213}Bi with a mean tissue range of 81 {mu}m may have a more favourable toxicity profile. Therefore, we evaluated locally injected {sup 213}Bi-DOTA-substance P in patients with critically located gliomas as the primary therapeutic modality. In a pilot study, we included five patients with critically located gliomas (WHO grades II-IV). After diagnosis by biopsy, {sup 213}Bi-DOTA-substance P was locally injected, followed by serial SPECT/CT and MR imaging and blood sampling. Besides feasibility and toxicity, the functional outcome was evaluated. Targeted radiopeptide therapy using {sup 213}Bi-DOTA-substance P was feasible and tolerated without additional neurological deficit. No local or systemic toxicity was observed. {sup 213}Bi-DOTA-substance P showed high retention at the target site. MR imaging was suggestive of radiation-induced necrosis and demarcation of the tumours, which was validated by subsequent resection. This study provides proof of concept that targeted local radiotherapy using {sup 213}Bi-DOTA-substance P is feasible and may represent an innovative and effective treatment for critically located gliomas. Primarily non-operable gliomas may become resectable with this treatment, thereby possibly improving the prognosis. (orig.)

  9. Delineation of a de novo 7q21.3q31.1 Deletion by CGH-SNP Arrays in a Girl with Multiple Congenital Anomalies Including Severe Glaucoma

    Martínez-Jacobo, L.; Córdova-Fletes, C.; Ortiz-López, R.; Rivas, F; Saucedo-Carrasco, C.; Rojas-Martínez, A.

    2013-01-01

    In this study, we present a female patient with a constitutional de novo deletion in 7q21.3q31.1 as determined by G-banding and CGH-SNP arrays. She exhibited, among other features, psychomotor retardation, congenital severe bilateral glaucoma, a cleft palate, and heart defect. Microarray assay disclosed a deleted 12.5-Mb region roughly 88 kb downstream the ectrodactyly critical region; thus, the patient's final karyotype was 46,XX.arr 7q21.3q31.1(96,742,140-109,246,085)×1 dn. This girl repres...

  10. Protection level of AI H5N1 vaccine clade 2.1.3 commercial against AI H5N1 clade 2.3.2 virus from Ducks to SPF chicken in laboratory conditions

    Indriani R

    2015-03-01

    Full Text Available Highly Pathogenic Avian Influenza (HPAI subtype H5N1 clade 2.3.2 has infected chickens in farms, causing mortality and a decrease in egg production. Vaccination is one of the strategies to control disease of AI subtype H5N1. AI H5N1 clade 2.1.3 vaccine is available commercially. The effectiveness of two vaccines of AI H5N1 clade 2.1.3 (product A and B, and AI H5N1 clade 2.3.2 (Sukoharjo against AI H5N1 clade 2.3.2 (Sukoharjo virus SPF chickens was tested in laboratory. Four groups of SPF chickens were used in this study, there were (1 vaccinated with H5N1 clade 2.1.3 (product A, (2 vaccinated with H5N1 clade 2.1.3 (product B, (3 vaccinated with AI H5N1 clade 2.3.2 and (4 unvaccinated (as a control. Each vaccinated group consisted of 10 chicken except 8 chicken for control group. SPF chicken were vaccinated with 1 dose of vaccine at 3 weeks olds, and then after 3 weeks post vaccination (at 6 weeks olds. All group of chicken were challenged with 106 EID50 per 0.1 ml via intranasal. The results showed, chicken vaccinated with H5N1 clade 2.1.3 product A and B gave 100 and 80% protection respectively, but showed challenged virus shedding, whereas vaccine of H5N1 clade 2.3.2 gave 100% protection from mortality and without virus shedding. Vaccines of AI H5N1 clade 2.1.3 product A was better than vaccine product B, and when chicken vaccinated against H5N1 clade 2.3.2, H5N1 clade 2.3.2 vaccine was the best to be used. In order to protect chicken from AI subtype H5N1 clade 2.1.3 and 2.3.2 in the field, a bivalent vaccine of H5N1 clade 2.1.3 and 2.3.2 subtypes should be developed.

  11. Single-dose anti-CD138 radioimmunotherapy: bismuth-213 is more efficient than lutetium-177 for treatment of multiple myeloma in a preclinical model

    Nolwenn eFichou

    2015-11-01

    Full Text Available Objectives: Radioimmunotherapy (RIT has emerged as a potential treatment option for multiple myeloma (MM. In humans, a dosimetry study recently showed the relevance of RIT using an antibody targeting the CD138 antigen. The therapeutic efficacy of RIT using an anti-CD138 antibody coupled to 213Bi, an α-emitter, was also demonstrated in a preclinical MM model. Since then, RIT with β-emitters has shown efficacy in treating hematologic cancer. In this paper, we investigate the therapeutic efficacy of RIT in the 5T33 murine MM model using a new anti-CD138 monoclonal antibody labeled either with 213Bi for α-RIT or 177Lu for β-RIT.Methods: A new monoclonal anti-CD138 antibody, 9E7.4, was generated by immunizing a rat with a murine CD138-derived peptide. Antibody specificity was validated by flow cytometry, biodistribution and α-RIT studies. Then, a β-RIT dose-escalation assay with the 177Lu-radiolabeled 9E7.4 mAb was performed in KalwRij C57/BL6 mice 10 days after i.v. engraftment with 5T33 MM cells. Animal survival and toxicological parameters were assessed to define the optimal activity.Results: α-RIT performed with 3.7 MBq of 213Bi-labeled 9E7.4 anti-CD138 mAb increased median survival to 80 days compared to 37 days for the untreated control and effected cure in 45% of animals. β-RIT performed with 18.5 MBq of 177Lu-labeled 9E7.4 mAb was well tolerated and significantly increased mouse survival (54 versus 37 days in the control group; however, no mice were cured with this treatment.Conclusion: This study revealed the advantages of α-RIT in the treatment of MM in a preclinical model where β-RIT shows almost no efficacy.

  12. A disseminated alveolar rhabdomyosarcoma in a 9-year-old boy disclosed by chromosomal translocation (2;13) (q35;q14)

    Brichard, B; Ninane, J; Gosseye, S; Verellen-Dumoulin, C; Vermylen, C; Rodhain, J; Cornu, G

    1991-01-01

    A 9-year-old boy presented with a small subcutaneous tumor of the trunk and diffuse bone marrow involvement. The first histological diagnosis given was undifferentiated malignancy possibly of neural crest origin and chemotherapy was started immediately using vincristine, cyclophosphamide, cisplatin, and teniposide (OPEC). Complete response was achieved after four courses of chemotherapy. Histological slides were then reviewed and the final diagnosis of alveolar rhabdomyosarcoma (RMS) was retained. Moreover, chromosome analysis of malignant cells in the bone marrow revealed a translocation involving chromosomes 2 and 13:t(2;13) (q35;q14). This specific karyotype finding has been recently reported in a few cases and could be specific for alveolar RMS. The patient had a relapse 7 months after diagnosis and died 4 months later. PMID:1742179

  13. Isolation of the human MOX2 homeobox gene and localization to chromosome 7p22.1-p21.3.

    Grigoriou, M; Kastrinaki, M C; Modi, W S; Theodorakis, K; Mankoo, B; Pachnis, V; Karagogeos, D

    1995-04-10

    We have isolated and characterized cDNA clones encoding a novel human homeobox gene, MOX2, the homologue of the murine mox-2 gene. The MOX2 protein contains all of the characteristic features of Mox-2 proteins of other vertebrate species, namely the homeobox, the polyhistidine stretch, and a number of potential serine/threonine phosphorylation sites. The homeodomain of MOX2 protein is identical to all other vertebrate species reported so far (rodents and amphibians). Outside the homeodomain, Mox-2 proteins share a high degree of identity, except for a few amino acid differences encountered between the human and the rodent polypeptides. A polyhistidine stretch of 12 amino acids in the N terminal region of the protein is also conserved among humans, rodents, and (only partly) amphibians. The chromosomal position of MOX2 was assigned to 7p22.1-p21.3. PMID:7607679

  14. Isolation of the human MOX2 homeobox gene and localization to chromosome 7p22.1-p21.3

    Grigoriou, M.; Theodorakis, K. [Institute of Molecular Biology and Biotechnology, Crete (Greece); Mankoo, B. [National Institute for Medical Research, London (United Kingdom)] [and others

    1995-04-10

    We have isolated and characterized cDNA clones encoding a novel human homeobox gene, MOX2, the homologue of the murine mox-2 gene. The MOX2 protein contains all of the characteristic features of Mox-2 proteins of other vertebrate species, namely the homeobox, the polyhistidine stretch, and a number of potential serine/threonine phosphorylation sites. The homeodomain of MOX2 protein is identical to all other vertebrate species reported so far (rodents and amphibians). Outside the homeodomain, Mox-2 proteins share a high degree of identity, except for a few amino acid differences encountered between the human and the rodent polypeptides. A polyhistidine stretch of 12 amino acids in the N terminal region of the protein is also conserved among humans, rodents, and (only partly) amphibians. The chromosomal position of MOX2 was assigned to 7p22.1-p21.3. 31 refs., 3 figs.

  15. Report of a consultants meeting on backfittings and safety enhancement measures in NPPs with WWER 440/213 reactors. Extrabudgetary programme on the safety of WWER NPPS

    The purpose of this Consultants' Meeting held by the IAEA in Vienna from 11-15 April 1994 within the framework of the Extrabudgetary Programme on WWER Safety was to review and analyze safety issues revealed during operation and through analyses of NPPs with WWER 440/213 reactors. The initial list of safety issues based on the available reports from various studies had been prepared by the IAEA secretariat before the meeting, together with indications of safety enhancement measures proposed in various NPP units. During the meeting, the underlying safety concerns and actual technical status of the plants were discussed and the ranking of the safety issues was considered. 58 refs, 1 tab

  16. Chloridobis[2-(1,3-thiazol-4-yl-κN-1H-benzimidazole-κN3]cobalt(II chloride dihydrate

    Zhan-Wang Shi

    2012-08-01

    Full Text Available In the title compound, [CoCl(C10H7N3S2]Cl·2H2O, the CoII atom is five-coordinated by four N atoms from two chelating 2-(1,3-thiazol-4-yl-1H-benzimidazole ligands and one Cl atom in a distorted trigonal–bipyramidal geometry. In the crystal, N—H...O and O—H...Cl hydrogen bonds and π–π interactions between the thiazole, imidazole and benzene rings [centroid-to-centroid distances 3.546 (2, 3.683 (2 and 3.714 (2 Å] link the complex cations, chloride anions and uncoordinating water molecules into a three-dimensional network.

  17. Preparation of 2'-13C-L-Histidine Starting from 13C-Thiocyanate: Synthetic Access to Any Site-Directed Stable Isotope Enriched L-Histidine

    Sarra Talab

    2014-01-01

    Full Text Available 1-Benzyl-2-(methylthio-imidazole-5-ketone is obtained in a few simple steps starting from thiocyanate and glycine amide (glycin. Subsequent treatment with diethyl phosphorocyanidate and functional group manipulations gives 1-benzyl-5-chloromethyl-imidazolium chloride. This compound is converted under mild O’Donnell conditions into the corresponding L-histidine derivative. After deprotection L-histidine is obtained in good yield and 99% enantiomeric excess. 2'-13C-L-Histidine has been obtained via this new scheme with high (99% 13C incorporation starting with commercially available 13C- thiocyanate. This synthetic scheme allows access to any isotopomer of L-histidine and many other biologically important imidazole derivatives.

  18. The light-yield response of a NE-213 liquid-scintillator detector measured using 2 -- 6 MeV tagged neutrons

    Scherzinger, J; Annand, J R M; Fissum, K G; Hall-Wilton, R; Kanaki, K; Lundin, M; Nilsson, B; Perrey, H; Rosborg, A; Svensson, H

    2016-01-01

    The response of a NE-213 liquid-scintillator detector has been measured using tagged neutrons from 2--6 MeV originating from an Am/Be neutron source. The neutron energies were determined using the time-of-flight technique. Pulse-shape discrimination was employed to discern between gamma-rays and neutrons. The behavior of both the fast (35 ns) and the combined fast and slow (475 ns) components of the neutron scintillation-light pulses were studied. Three different prescriptions were used to relate the neutron maximum energy-transfer edges to the corresponding recoil-proton scintillation-light yields, and the results were compared to simulations. Parametrizations which predict the fast or total light yield of the scintillation pulses were also tested. Our results agree with both existing data and existing parametrizations. We observe a clear sensitivity to the portion and length of the neutron scintillation-light pulse considered.

  19. 4-(5-甲醛基-2-噻吩基)-7-(2-噻吩基)[2,1,3]苯并噻二唑的合成%Synthesis of 4-(5-formyl-2-thienyl)-7-(2-thienyl)[2,1,3] benzothiadiazole

    李彦军; 陈功; 黄鹏程

    2007-01-01

    采用两种不同的途径合成了4-(5-甲醛基-2-噻吩基)-7-(2-噻吩基)[2,1,3]苯并噻二唑.研究结果表明,DMF/POCl3体系较n-BuLi/DMF体系产率更高.改变两种合成途径的反应条件,或用单醛化产物进一步醛化,均未发现二醛取代产物的生成.

  20. Response function of NE213 scintillator for 0.5-6 MeV neutrons measured by an improved pulse shape discrimination

    Using the pulse shape discrimination method combined with the time of flight technique, we have obtained the response function of a 2'' diameter x 2'' thick NE213 scintillator by measuring directly the proton recoil energy spectrum of 0.5-6 MeV prompt neutrons from a 252Cf source. Three parameters, time of flight (TOF), pulse shape discrimination (PSD) and recoil energy (RE), were recorded in an event-by-event mode with a TOF gate. We attempted to improve the determination of maximum proton recoil energies equal to incident neutron energies by employing two analysis methods. First, we attempted to separate better neutrons from coexisting gamma rays in the PSD spectrum by projecting neutron channels of the PSD spectrum onto both TOF and RE spectra in a cubic matrix constructed by the three-parameter data. The resulting two-dimensional matrix composed of TOF and RE channels was free from gamma rays and corresponding Compton-recoiled electrons, and then projected with neutron energy bins of 0.05 MeV wide determined by TOF. Finally, to determine the maximum proton recoil energies from each RE spectrum with a realistic function, accounting for the nonlinear response of the NE213 scintillator due to the quenching effect, we performed a least-squares fit to the RE spectrum using the four-parameter function. The response function obtained in the present work agrees well with previous experimental results obtained by Gul et al. (Nucl. Instr. and Meth. A 278 (1989) 470) and a Monte Carlo study by Cecil et al. (Nucl. Instr. and Meth. 161 (1979) 439). (orig.)

  1. SPECTROSCOPIC CONFIRMATION OF THREE z-DROPOUT GALAXIES AT z = 6.844-7.213: DEMOGRAPHICS OF Ly{alpha} EMISSION IN z {approx} 7 GALAXIES

    Ono, Yoshiaki; Shimasaku, Kazuhiro; Nakajima, Kimihiko, E-mail: ono@astron.s.u-tokyo.ac.jp [Department of Astronomy, Graduate School of Science, University of Tokyo, Tokyo 113-0033 (Japan); Ouchi, Masami [Institute for Cosmic Ray Research, University of Tokyo, Kashiwa 277-8582 (Japan); Mobasher, Bahram; Nayyeri, Hooshang [Department of Physics and Astronomy, University of California, Riverside, CA 92521 (United States); Dickinson, Mark; Kartaltepe, Jeyhan S. [National Optical Astronomical Observatories, Tucson, AZ 85719 (United States); Penner, Kyle [Department of Astronomy, University of Arizona, Tucson, AZ 85721 (United States); Weiner, Benjamin J. [Steward Observatory, University of Arizona, Tucson, AZ 85721 (United States); Stern, Daniel [Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109 (United States); Kashikawa, Nobunari [Optical and Infrared Astronomy Division, National Astronomical Observatory of Japan, Tokyo 181-8588 (Japan); Spinrad, Hyron [Department of Astronomy, University of California, Berkeley, CA 94720 (United States)

    2012-01-10

    We present the results of our ultra-deep Keck/DEIMOS spectroscopy of z-dropout galaxies in the Subaru Deep Field and Great Observatories Origins Deep Survey's northern field. For 3 out of 11 objects, we detect an emission line at {approx}1 {mu}m with a signal-to-noise ratio of {approx}10. The lines show asymmetric profiles with high weighted skewness values, consistent with being Ly{alpha}, yielding redshifts of z = 7.213, 6.965, and 6.844. Specifically, we confirm the z = 7.213 object in two independent DEIMOS runs with different spectroscopic configurations. The z = 6.965 object is a known Ly{alpha} emitter, IOK-1, for which our improved spectrum at a higher resolution yields a robust skewness measurement. The three z-dropouts have Ly{alpha} fluxes of 3 Multiplication-Sign 10{sup -17} erg s{sup -1} cm{sup -2} and rest-frame equivalent widths EW{sup Ly{alpha}}{sub 0} = 33-43 A. Based on the largest spectroscopic sample of 43 z-dropouts, which is the combination of our and previous data, we find that the fraction of Ly{alpha}-emitting galaxies (EW{sup Ly{alpha}}{sub 0} > 25 A) is low at z {approx} 7; 17% {+-} 10% and 24% {+-} 12% for bright (M{sub UV} {approx_equal} -21) and faint (M{sub UV} {approx_equal} -19.5) galaxies, respectively. The fractions of Ly{alpha}-emitting galaxies drop from z {approx} 6 to 7 and the amplitude of the drop is larger for faint galaxies than for bright galaxies. These two pieces of evidence would indicate that the neutral hydrogen fraction of the intergalactic medium increases from z {approx} 6 to 7 and that the reionization proceeds from high- to low-density environments, as suggested by an inside-out reionization model.

  2. Selective T-cell Ablation with Bismuth-213 Labeled Anti-TCR Alpha Beta as Nonmyeloablative Conditionaing for Allogeneic Canine Marrow Transplantion

    Bethge, W. A.; Wilbur, D. Scott; Storb, R.; Hamlin, Donald K.; Santos, E. B.; Brechbiel, M. W.; Fisher, Darrell R.; Sandmaier, B. M.

    2003-06-15

    Two major immunological barriers, the host versus graft (HVG) and the graft versus host (GVH) reaction, must be overcome for successful allogeneic hematopoietic stem cell transplantation. T-cells are involved in these barriers in the major histocompatibility complex-identical settings. We hypothesized that selective ablation of T-cells using radioimmunotherapy, together with postgrafting immunosuppression, would ensure stable allogeneic engraftment. We developed a canine model of nonmyeloablative marrow transplantation in which host immune reactions are impaired by a single dose of 2 Gy total body irradiation (TBI), and where both GVH and residual HVG reactions are controlled by postgrafting immunosuppression with mycophenolate mofetil (MMF) and cyclosporine (CSP). We substituted the alpha-emitter bismuth-213 linked to a monoclonal antibody against TCR(alpha,beta)using the metal-binding chelate CHX-A”-DTPA, for 2 Gy TBI. Biodistribution studies using a gamma-emitting indium-111-labeled anti-TCR mAb showed uptake primarily in blood, marrow, lymph nodes, spleen and liver. In a dosimetry study, 4 dogs were treated with 0.13-0.46 mg/kg TCR mAb labeled with 3.7-5.6 mCi/kg (137-207 MBq/kg) Bi-213. The treatment was administered in 6 injections on days -3 and -2 followed by transplantion of dog leukocyte antigen-identical marrow on day 0 and postgrafting immunosuppression with MMF and CSP. Therapy was well tolerated except for elevations of transaminases, which were transient in all but one dog. No other organ toxicities or signs of graft-versus-host-disease were noted. The dogs had prompt allogeneic hematopoietic engraftment and achieved stable mixed donor-host hematopoietic chimerism with donor contributions ranging from 5-55 % with >30 weeks follow up.

  3. SPECTROSCOPIC CONFIRMATION OF THREE z-DROPOUT GALAXIES AT z = 6.844-7.213: DEMOGRAPHICS OF Lyα EMISSION IN z ∼ 7 GALAXIES

    We present the results of our ultra-deep Keck/DEIMOS spectroscopy of z-dropout galaxies in the Subaru Deep Field and Great Observatories Origins Deep Survey's northern field. For 3 out of 11 objects, we detect an emission line at ∼1 μm with a signal-to-noise ratio of ∼10. The lines show asymmetric profiles with high weighted skewness values, consistent with being Lyα, yielding redshifts of z = 7.213, 6.965, and 6.844. Specifically, we confirm the z = 7.213 object in two independent DEIMOS runs with different spectroscopic configurations. The z = 6.965 object is a known Lyα emitter, IOK-1, for which our improved spectrum at a higher resolution yields a robust skewness measurement. The three z-dropouts have Lyα fluxes of 3 × 10–17 erg s–1 cm–2 and rest-frame equivalent widths EWLyα0 = 33-43 Å. Based on the largest spectroscopic sample of 43 z-dropouts, which is the combination of our and previous data, we find that the fraction of Lyα-emitting galaxies (EWLyα0 > 25 Å) is low at z ∼ 7; 17% ± 10% and 24% ± 12% for bright (MUV ≅ –21) and faint (MUV ≅ –19.5) galaxies, respectively. The fractions of Lyα-emitting galaxies drop from z ∼ 6 to 7 and the amplitude of the drop is larger for faint galaxies than for bright galaxies. These two pieces of evidence would indicate that the neutral hydrogen fraction of the intergalactic medium increases from z ∼ 6 to 7 and that the reionization proceeds from high- to low-density environments, as suggested by an inside-out reionization model.

  4. A Novel Cryptic Three-Way Translocation t(2;9;18(p23.2;p21.3;q21.33 with Deletion of Tumor Suppressor Genes in 9p21.3 and 13q14 in a T-Cell Acute Lymphoblastic Leukemia

    Moneeb A. K. Othman

    2014-01-01

    Full Text Available Acute leukemia often presents with pure chromosomal resolution; thus, aberrations may not be detected by banding cytogenetics. Here, a case of 26-year-old male diagnosed with T-cell acute lymphoblastic leukemia (T-ALL and a normal karyotype after standard GTG-banding was studied retrospectively in detail by molecular cytogenetic and molecular approaches. Besides fluorescence in situ hybridization (FISH, multiplex ligation-dependent probe amplification (MLPA and high resolution array-comparative genomic hybridization (aCGH were applied. Thus, cryptic chromosomal aberrations not observed before were detected: three chromosomes were involved in a cytogenetically balanced occurring translocation t(2;9;18(p23.2;p21.3;q21.33. Besides a translocation t(10;14(q24;q11 was identified, an aberration known to be common in T-ALL. Due to the three-way translocation deletion of tumor suppressor genes CDKN2A/INK4A/p16, CDKN2B/INK4B/p15, and MTAP/ARF/p14 in 9p21.3 took place. Additionally RB1 in 13q14 was deleted. This patient, considered to have a normal karyotype after low resolution banding cytogenetics, was treated according to general protocol of anticancer therapy (ALL-BFM 95.

  5. MicroRNA-21-3p, a berberine-induced miRNA, directly down-regulates human methionine adenosyltransferases 2A and 2B and inhibits hepatoma cell growth.

    Ting-Fang Lo

    Full Text Available Methionine adenosyltransferase (MAT is the cellular enzyme that catalyzes the synthesis of S-adenosylmethionine (SAM, the principal biological methyl donor and a key regulator of hepatocyte proliferation, death and differentiation. Two genes, MAT1A and MAT2A, encode 2 distinct catalytic MAT isoforms. A third gene, MAT2B, encodes a MAT2A regulatory subunit. In hepatocellular carcinoma (HCC, MAT1A downregulation and MAT2A upregulation occur, known as the MAT1A:MAT2A switch. The switch is accompanied with an increasing expression of MAT2B, which results in decreased SAM levels and facilitates cancer cell growth. Berberine, an isoquinoline alkaloid isolated from many medicinal herbs such as Coptis chinensis, has a wide range of pharmacological effects including anti-cancer effects. Because drug-induced microRNAs have recently emerged as key regulators in guiding their pharmacological effects, we examined whether microRNA expression is differentially altered by berberine treatment in HCC. In this study, we used microRNA microarrays to find that the expression level of miR-21-3p (previously named miR-21* increased after berberine treatment in the HepG2 human hepatoma cell line. To predict the putative targets of miR-21-3p, we integrated the gene expression profiles of HepG2 cells after berberine treatment by comparing with a gene list generated from sequence-based microRNA target prediction software. We then confirmed these predictions through transfection of microRNA mimics and a 3' UTR reporter assay. Our findings provide the first evidence that miR-21-3p directly reduces the expression of MAT2A and MAT2B by targeting their 3' UTRs. In addition, an overexpression of miR-21-3p increased intracellular SAM contents, which have been proven to be a growth disadvantage for hepatoma cells. The overexpression of miR-21-3p suppresses growth and induces apoptosis in HepG2 cells. Overall, our results demonstrate that miR-21-3p functions as a tumor suppressor

  6. Final Report for research grant entitled "Development of Reagents for Application of At-211 and Bi-213 to Targeted Radiotherapy of Cancer"

    Wilbur, D. Scott

    2011-12-23

    This grant was a one-year extension of another grant with the same title (DE-FG03-98ER62572). The objective of the studies was to continue in vivo evaluation of reagents to determine which changes in structure were most favorable for in vivo use. The focus of our studies was development and optimization of reagents for pretargeting alpha-emitting radionuclides At-211 or Bi-213 to cancer cells. Testing of the reagents was conducted in vitro and in animal model systems. During the funding period, all three specific aims set out in the proposed studies were worked on, and some additional studies directed at development of a method for direct labeling of proteins with At-211 were investigated. We evaluated reagents in two different approaches in 'two step' pretargeting protocols. These approaches are: (1) delivery of the radionuclide on recombinant streptavidin to bind with pretargeted biotinylated monoclonal antibody (mAb), and alternatively, (2) delivery of the radionuclide on a biotin derivative to bind with pretargeted antibody-streptavidin conjugates. The two approaches were investigated as it was unclear which will be superior for the short half-lived alpha-emitting radionuclides.

  7. Possible pressurized thermal shock events during large primary to secondary leakage. The Hungarian AGNES project and PRISE accident scenarios in VVER-440/V213 type reactor

    Perneczky, L. [KFKI Atomic Energy Research Inst., Budabest (Hungary)

    1997-12-31

    Nuclear power plants of WWER-440/213-type have several special features. Consequently, the transient behaviour of such a reactor system should be different from the behaviour of the PWRs of western design. The opening of the steam generator (SG) collector cover, as a specific primary to secondary circuit leakage (PRISE) occurring in WWER-type reactors happened first time in Rovno NPP Unit I on January 22, 1982. Similar accident was studied in the framework of IAEA project RER/9/004 in 1987-88 using the RELAP4/mod6 code. The Hungarian AGNES (Advanced General and New Evaluation of Safety) project was performed in the period 1991-94 with the aim to reassess the safety of the Paks NPP using state-of-the-art techniques. The project comprised three type of analyses for the primary to secondary circuit leakages: Design Basis Accident (DBA) analyses, Pressurized Thermal Shock (PTS) study and deterministic analyses for Probabilistic Safety Analysis (PSA). Major part of the thermohydraulic analyses has been performed by the RELAP5/mod2.5/V251 code version with two input models. 32 refs.

  8. Determination of medroxyprogesterone acetate in serum by HPLC with peroxyoxalate chemiluminescence detection using a fluorogenic reagent, 4-(N,N-dimethylaminosulphonyl)-7-hydrazino-2,1,3-benzoxadiazole.

    Uzu, S; Imai, K; Nakashima, K; Akiyama, S

    1992-01-01

    The high-performance liquid chromatographic determination of medroxyprogesterone acetate (MPA) with peroxyoxalate chemiluminescence (PO-CL) detection is described. The spiked serum containing MPA was extracted on Bond-Elut C18 columns and derivatized with 4-(N,N-dimethylaminosulphonyl)-7-hydrazino-2,1,3-benzoxadiazole (DBD-H). The hydrazone of MPA with DBD-H was confirmed to be a mono-DBD-derivative. The reaction mixture was separated by direct injection onto a C18 analytical column, and quantified by PO-CL detection. The linear range of the standard curve, in serum, was 15.6-96.6 ng ml-1 with a detection limit of 9 ng ml-1 using only 100 microliters of serum, while the detection limit of standard MPA derivatized with DBD-H was 8.7 fmol per injection. The relative standard deviation of the method was 7.4% at 19.3 ng and 1.7% at 77.3 ng ml-1. PMID:1298405

  9. Sub-Doppler two-photon spectroscopy of 33 Rydberg levels in atomic xenon excited at 205–213 nm: diverse isotopic and hyperfine structure

    Isotope energy shifts and hyperfine structure have been measured for 33 high-energy Rydberg levels of atomic xenon by sub-Doppler two-photon excitation spectroscopy, using narrowband pulses of coherent ultraviolet light at 205–213 nm generated by nonlinear-optical conversion processes. Rydberg levels are accessed at two-photon excitation energies in the 97 300–94 100 cm−1 range where isotope energy shifts and hyperfine structure have rarely been resolved; these Rydberg levels are 5p5 np [1/2]0 (n = 9–13), 5p5 np [3/2]2 (n = 9–13), 5p5 np [5/2]2 (n = 9–17), 5p5 nf [3/2]2 (n = 6–14) and 5p5 nf [5/2]2 (n = 6–10). The sub-Doppler spectra display diverse hyperfine-coupling effects, for which least-squares-fit spectroscopic parameters reflect the influence of angular momentum. (paper)

  10. Corrosión por depósitos salinos de los aceros SA213-T22 y SA213-TP347H en presencia de una mezcla 80%V2O5-20%Na2SO4-20%Na2SO4

    Romero, M. A.

    1998-02-01

    Full Text Available Many hot corrosion problems in industrial and utility boilers are caused by molten salts. The corrosion processes which occur in salts are of an electrochemical nature, and so they can be studied using electrochemical test methods. In this research, electrochemical techniques in molten salt systems have been used for the measurements of molten corrosion processes. Electrochemical test methods are described here for a salt mixture of 80%V2O5-20%Na2SO4 at 540-680°C. To establish better the electrochemical corrosion rate mearurements for molten salt systems, information from electrochemical potentiodynamic polarization curves, such as polarization resistance and Tafel slopes were used in this study to generate corrosion rate data. The salt was contained in a quartz crucible inside a stainless steel retort. The atmosphere used was air. A thermocouple sheathed with quartz glass was introduced into the molten salt for temperature monitoring and control. Two materials were tested in the molten mixture: SA213-T22 and SA213-TP347H steels. The corrosion rates values obtained using electrochemical methods were around 0.58-7.14 mm/yr (22.9-281 mpy. The corrosion rate increase with time.Muchos problemas de corrosión por depósitos salinos en la industria eléctrica, especialmente en los generadores de vapor, se deben al ataque por sales fundidas. El proceso de corrosión en sales fundidas es de naturaleza electroquímica; así, puede estudiarse empleando técnicas electroquímicas. Este proceso de corrosión en un sistema de sales fundidas, mezcla de 80%V2O5 y 20%Na2SO4 a temperaturas comprendidas entre 540 y 680°C, se evaluó en esta investigación por medio de técnicas electroquímicas. Para medir la velocidad de corrosión por depósitos salinos se parte de curvas de polarización potenciodinámicas determinando la resistencia de polarización por extrapolación de las pendientes de Tafel. Las sales se fundieron en un crisol de cuarzo dentro de un

  11. Delineation of a de novo 7q21.3q31.1 Deletion by CGH-SNP Arrays in a Girl with Multiple Congenital Anomalies Including Severe Glaucoma.

    Martínez-Jacobo, L; Córdova-Fletes, C; Ortiz-López, R; Rivas, F; Saucedo-Carrasco, C; Rojas-Martínez, A

    2013-09-01

    In this study, we present a female patient with a constitutional de novo deletion in 7q21.3q31.1 as determined by G-banding and CGH-SNP arrays. She exhibited, among other features, psychomotor retardation, congenital severe bilateral glaucoma, a cleft palate, and heart defect. Microarray assay disclosed a deleted 12.5-Mb region roughly 88 kb downstream the ectrodactyly critical region; thus, the patient's final karyotype was 46,XX.arr 7q21.3q31.1(96,742,140-109,246,085)×1 dn. This girl represents the fourth patient described so far with congenital glaucoma and a deletion encompassing or overlapping the 7q21.3q31.1 region, and confirms the presence of a locus or loci related to such a clinical feature. According to our results, the proneness to ocular defects secondary to 7q intermediate deletions could be caused by co-deletion of TAC1, HBP1, and a small cluster of cytochrome P450 genes (subfamily 3A). This conclusion is supported by their functional roles and expression locations as well as because TAC1 is related to the functional pathway of the MYOC gene whose mutations are linked to glaucoma. Moreover, given that this girl is clinically reminiscent of several phenotypes related to diverse deletions within 7q21q32, our results and observations offer a general overview of the gene content of deletions/phenotypes overlapping 7q21.3q31.1 and confirm that loci distal to DLX genes including the CUX1 gene and potential regulatory elements downstream from DLX5 are unrelated to ectrodactyly. PMID:24167464

  12. System for recovery of daughter isotopes from a source material

    Tranter, Troy J [Idaho Falls, ID; Todd, Terry A [Aberdeen, ID; Lewis, Leroy C [Idaho Falls, ID; Henscheid, Joseph P [Idaho Falls, ID

    2009-08-04

    A method of separating isotopes from a mixture containing at least two isotopes in a solution is disclosed. A first isotope is precipitated and is collected from the solution. A daughter isotope is generated and collected from the first isotope. The invention includes a method of producing an actinium-225/bismuth-213 product from a material containing thorium-229 and thorium-232. A solution is formed containing nitric acid and the material containing thorium-229 and thorium-232, and iodate is added to form a thorium iodate precipitate. A supernatant is separated from the thorium iodate precipitate and a second volume of nitric acid is added to the thorium iodate precipitate. The thorium iodate precipitate is stored and a decay product comprising actinium-225 and bismuth-213 is generated in the second volume of nitric acid, which is then separated from the thorium iodate precipitate, filtered, and treated using at least one chromatographic procedure. A system for producing an actinium-225/bismuth-213 product is also disclosed.

  13. In vitro evaluation of {sup 213}Bi-rituximab versus external gamma irradiation for the treatment of B-CLL patients: relative biological efficacy with respect to apoptosis induction and chromosomal damage

    Vandenbulcke, Katia; Lahorte, Christophe; Slegers, Guido [Department of Radiopharmacy, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000, Gent (Belgium); De Vos, Filip; Dierckx, Rudi A. [Division of Nuclear Medicine, Ghent University Hospital (Belgium); Offner, Fritz [Department of Hematology, Ghent University Hospital (Belgium); Philippe, Jan [Department of Clinical Chemistry, Ghent University Hospital (Belgium); Apostolidis, Christos; Molinet, Roger; Nikula, Tuomo K. [European Commission, Joint Research Centre, Institute for Transuranium Elements, Karlsruhe (Germany); Bacher, Klaus; De Gelder, Virginie; Vral, Anne; Thierens, Hubert [Department of Anatomy, Embryology, Histology and Medical Physics, Ghent University (Belgium)

    2003-10-01

    External source radiotherapy and beta radioimmunotherapy (RIT) are effective treatments for lymphoid malignancies. The development of RIT with alpha emitters is attractive because of the high linear energy transfer (LET) and short path length, allowing higher tumour cell kill and lower toxicity to healthy tissues. We assessed the relative biological efficacy (RBE) of alpha RIT (in vitro) compared to external gamma irradiation with respect to induction of apoptosis in B chronic lymphocytic leukaemia (B-CLL) and induction of chromosomal damage in healthy donor B and T lymphocytes. The latter was measured by a micronucleus assay. {sup 213}Bi was eluted from a {sup 225}Ac generator and conjugated to CD20 antibody (rituximab) with CHX-A''-DTPA as a chelator. B-CLL cells from five patients were cultured for 24 h in RPMI/10% FCS while exposed to {sup 213}Bi conjugated to CD20 antibody or after external {sup 60}Co gamma irradiation. Binding assays were performed in samples of all patients to calculate the total absorbed dose. Apoptosis was scored by flow cytometric analyses of the cells stained with annexin V-FITC and 7-AAD. Apoptosis was expressed as % excess over spontaneous apoptosis in control. Full dose range experiments demonstrated {sup 213}Bi-conjugated CD20 antibody to be more effective than equivalent doses of external gamma irradiation, but showed that similar plateau values were reached at 10 Gy. The RBE for induction of apoptosis in B-CLL was 2 between 1.5 and 7 Gy. The micronucleus yield in lymphocytes of healthy volunteers was measured to assess the late toxicity caused by induction of chromosomal instability. While gamma radiation induced a steady increase in micronucleus yields in B and T cells, the damage induced by {sup 213}Bi was more dramatic, with RBE ranging from 5 to 2 between 0.1 Gy and 2 Gy respectively. In contrast to gamma irradiation, {sup 213}Bi inhibited mitogen-stimulated mitosis almost completely at 2 Gy. In conclusion, high

  14. In vitro evaluation of 213Bi-rituximab versus external gamma irradiation for the treatment of B-CLL patients: relative biological efficacy with respect to apoptosis induction and chromosomal damage

    External source radiotherapy and beta radioimmunotherapy (RIT) are effective treatments for lymphoid malignancies. The development of RIT with alpha emitters is attractive because of the high linear energy transfer (LET) and short path length, allowing higher tumour cell kill and lower toxicity to healthy tissues. We assessed the relative biological efficacy (RBE) of alpha RIT (in vitro) compared to external gamma irradiation with respect to induction of apoptosis in B chronic lymphocytic leukaemia (B-CLL) and induction of chromosomal damage in healthy donor B and T lymphocytes. The latter was measured by a micronucleus assay. 213Bi was eluted from a 225Ac generator and conjugated to CD20 antibody (rituximab) with CHX-A''-DTPA as a chelator. B-CLL cells from five patients were cultured for 24 h in RPMI/10% FCS while exposed to 213Bi conjugated to CD20 antibody or after external 60Co gamma irradiation. Binding assays were performed in samples of all patients to calculate the total absorbed dose. Apoptosis was scored by flow cytometric analyses of the cells stained with annexin V-FITC and 7-AAD. Apoptosis was expressed as % excess over spontaneous apoptosis in control. Full dose range experiments demonstrated 213Bi-conjugated CD20 antibody to be more effective than equivalent doses of external gamma irradiation, but showed that similar plateau values were reached at 10 Gy. The RBE for induction of apoptosis in B-CLL was 2 between 1.5 and 7 Gy. The micronucleus yield in lymphocytes of healthy volunteers was measured to assess the late toxicity caused by induction of chromosomal instability. While gamma radiation induced a steady increase in micronucleus yields in B and T cells, the damage induced by 213Bi was more dramatic, with RBE ranging from 5 to 2 between 0.1 Gy and 2 Gy respectively. In contrast to gamma irradiation, 213Bi inhibited mitogen-stimulated mitosis almost completely at 2 Gy. In conclusion, high-LET targeted alpha particle exposure killed B-CLL cells

  15. LASER ASSISTED SUB EPITHELIAL KERATECTOMY FOR HYPEROPIA USING 213 NM WAVELENGTH SOLID STATE LASER, 3 YEAR FOLLOW UP STUDY OF REFRACTIVE AND VISUAL OUTCOMES

    Raman

    2015-07-01

    Full Text Available PURPOSE: To evaluate the visual and refractive outcomes, using 213 nm solid lasers for the correction of broad range of hyperopia, covering pediatric age group to presbyopic age group. MATERIALS AND METHODS: The Present study is a prospective case series in a private practice setup, analysed series of eyes, with low to high hyperopia, w ho underwent conventional LASEK. Long term (3 year visual and refractive outcomes and overall patient satisfaction studied. RESULTS: This cohort study of 37 eyes included broad range of hyperopia between +1.5 D to +7.5 D. For 70.27% of eyes, there was no change in CDVA post operatively and there was a significant Gain of one line in 21.62% of eyes and the paediatric eyes (4 gained one line. The post - op improvement in logMAR UDVA/decimal notations is statistically significant (P value <0.01. No significan t change was detected in logMAR CDVA/Decimal notations. The post op log MAR UDVA is +0.30 (20/40 in 78.4% of eyes, +0.48 (20/60 visual acuity in 95% of eyes. Post op LogMAR UDVA 0.00 (20/20 in 40.54% of eyes is equal to pre - op logMAR CDVA. The efficacy index is 1.01 and safety index is 0.99. Post op 3 eyes (8.11% lost 1 line of CDVA and 8 eyes (21.62% gained one line of Snellen’s visual acuity. CONCLUSION: The conventional LASEK can be performed using solid laser, safely and effectively for hyperopia c overing broad age groups and it can achieve effective visual outcomes with excellent overall patient satisfaction.

  16. Isolation of cosmid and cDNA clones in the region surrounding the BTK gene at Xq21.3-q22

    Vorechovsky, I.; Zhou, J.N.; Hammarstroem, L. [Karolinska Institute, Huddinge (Sweden)] [and others

    1994-06-01

    A regional physical and transcription map involving yeast artificial chromosomes (YACs), cosmids, and cDNAs has been constructed for Xq21.3-q22 around the gene BTK (formerly atk or BPK) defective in X-linked agammaglobulinemia (XLA). With a positional cloning strategy employing direct cDNA selection, novel cDNAs were found to cluster in the region of approximately 100 kb flanking the XLA and {alpha}-galactosidase A loci. While these widely expressed transcripts are in the area known to contain CpG islands, a less evolutionarily conserved gene, located more than 130 kb distal of DXS178, maps to cosmid clones that could not be digested with rare-cutting restriction enzymes. The presence of transcribed sequences flanking the BTK allowed investigation of their involvement in complex XLA phenotypes. Southern blot analysis using cDNA clones isolated from this region permitted exclusion of a contiguous deletion syndrome as an underlying defect in three patients with XLA and associated growth hormone deficiency. A single XLA patient with torsion dystonia and cosegregating X-linked deafness has been found with a deletion in the 3{prime} part of BTK extending centromerically into the flanking expressed sequence DXS1274E. This suggests a possible involvement of the DXS1274E in this phenotype. The GenBank accession numbers for novel cDNA sequences are as follows: DXS1269E (L20773), DXS1271E (UO1923), DXS1273E (UO1925), and DXS1274E (UO1922). 51 refs., 4 figs., 1 tab.

  17. Identification of a microdeletion at 7q21.3 with fluorescence in situ hybridization in a patient with split hand/split foot (ectrodactyly)

    Hudgins, L. [Children`s Hospital and Medical Center, Seattle, WA (United States); Massa, H.; Disteche, C. [Univ. of Washington, Seattle, WA (United States)] [and others

    1994-09-01

    Split hand/split foot (SHSF), often referred to as ectrodactyly or lobster claw deformity, is a human developmental disorder characterized by a deep median cleft of the hands and feet, missing digits, and fusion of remaining digits. This anomaly can be seen alone, frequently autosomal dominant, or in association with other abnormalities. One locus for this defect has been localized to chromosome 7q21.3-q22.1. We report a patient with SHSF plus mental retardation, short stature and dysmorphic features who was found to have a microdeletion at this locus detected only with the aid of fluorescence in situ hybridization (FISH). T.H. is a 7 y.o. male who was referred for evaluation of foot anomalies and mild mental retardation. History was remarkable for growth retardation of postnatal onset and hypotonia. Renal ultrasound and audiology evaluation were normal. Physical exam revealed dysplastic ears, micrognathia, long philtrum, high narrow palate, and malformations of the feet consistent with SHSF. Family history was negative for limb abnormalities and mental retardation. A number of patients with SHSF and other anomalies have been found to have deletions involving chromosome 7q21-q22; therefore, high resolution chromosome analysis was performed in T.H. but was inconclusive. Cosmids and yeast artificial chromosomes which we had previously mapped to the SHSF critical region were used as FISH probes and a microdeletion was detected. We were thus able to determine the etiology of this child`s abnormalities and provide accurate genetic counseling, which would not have been possible with standard cytogenetic techniques. This technique also allowed us to further refine the SHSF critical region. This case illustrates the utility of FISH for the rapid identification of suspect microdeletions in SHSF. This approach should also be useful as an expeditious way of defining the critical regions for the location of genes which give rise to other developmental malformations.

  18. Structural and functional studies of FKHR-PAX3, a reciprocal fusion gene of the t(2;13 chromosomal translocation in alveolar rhabdomyosarcoma.

    Qiande Hu

    Full Text Available Alveolar rhabdomyosarcoma (ARMS is an aggressive pediatric cancer of skeletal muscle. More than 70% of ARMS tumors carry balanced t(2;13 chromosomal translocation that leads to the production of two novel fusion genes, PAX3-FKHR and FKHR-PAX3. While the PAX3-FKHR gene has been intensely studied, the reciprocal FKHR-PAX3 gene has rarely been described. We report here the cloning and functional characterization of the FKHR-PAX3 gene as the first step towards a better understanding of its potential impact on ARMS biology. From RH30 ARMS cells, we detected and isolated three versions of FKHR-PAX3 cDNAs whose C-terminal sequences corresponded to PAX3c, PAX3d, and PAX3e isoforms. Unlike the nuclear-specific localization of PAX3-FKHR, the reciprocal FKHR-PAX3 proteins stayed predominantly in the cytoplasm. FKHR-PAX3 potently inhibited myogenesis in both non-transformed myoblast cells and ARMS cells. We showed that FKHR-PAX3 was not a classic oncogene but could act as a facilitator in oncogenic pathways by stabilizing PAX3-FKHR expression, enhancing cell proliferation, clonogenicity, anchorage-independent growth, and matrix adhesion in vitro, and accelerating the onset of tumor formation in xenograft mouse model in vivo. In addition to these pro-oncogenic behaviors, FKHR-PAX3 also negatively affected cell migration and invasion in vitro and lung metastasis in vivo. Taken together, these functional characteristics suggested that FKHR-PAX3 might have a critical role in the early stage of ARMS development.

  19. IMPACT OF LOCUS 9P21.3 SINGLE NUCLEOTIDE POLYMORPHISMS ON CORONARY ATHEROSCLEROSIS SEVERITY AND LONG-TERM OUTCOMES AFTER PERCUTANEOUS CORONARY INTERVENTION IN PATIENT WITH MYOCARDIAL INFARCTION

    P. A. Shesternya

    2015-09-01

    Full Text Available Aim. To investigate association between 9p21.3 locus single nucleotide polymorphisms (SNPs and coronary atherosclerosis severity and long-term outcomes after percutaneous coronary intervention (PCI in patients with myocardial infarction (MI.Material and methods. A total of 255 Caucasian patients (211 male, 44 female; aged up to 65 years, on the average 52.56±7.98 years with MI were recruited into the study from 01.01.2009 to 30.06.2010. All participants were included into the study after written informed consent. Genome DNA was extracted from leukocytes of venous blood by the phenol-chloroform extraction method. Two SNPs rs10757278 and rs1333049 (locus 9p21.3 were tested by real-time polymerase chain reaction (PCR according to protocol (probes TaqMan, Applied Biosystems, 7900HT. The coronary angiograms were reviewed by independent angiographers who were blinded to the results of the genotyp- ing (Philips Allura Xper FD10. The total number of lesions, Gensini score and SYNTAX score were derived. Follow-up lasted two years.Results. Locus 9р21.3 genotypes CC rs1333049 and GG rs10757278 demonstrated a direct strong association with severity of coronary atheromatous burden (left main coro- nary artery stenosis, total number of lesions, Gensini score. There are not influence of locus 9p21.3 on mortality, recurrent MI, hospitalization due to unstable angina, repeated PCI, stroke during follow-up period (6, 12, 24 months. Frequency of the genotype СС rs1333049 among patients with recurrent MI was 20% (without recurrent MI — 27.4%; р=0.54; with hospitalization due to unstable angina — 27.5% (without hospitalization — 26.4%; р=0.82; with repeated PCI — 24.0% (without repeated PCI — 27.2%; р=0.97; among died patients — 29.8% (among survived ones — 26.4%; р=0.76. Frequencies of the genotype GG rs10757278 were similar: recurrent MI (yes — 18.8%; no — 26.4%; р=0.49; hospitalization due to unstable angina (yes — 28%; no — 25

  20. 21.3.Other Psychosis

    1993-01-01

    930407 Lymphocyte β-adrenergic receptorfunction in depressed patients.FAN Xiaodong(笵肖冬),et al.Instit Ment Health,Beijing MedUniv,100083.Chin J Neurol & Psychiat 1992;25(6):322—324.Lymphocyte β-adrenergic receptor functionof 20 depressed patients and 18 healthy volun-teers were measured by using radio-ligandbinding technique.It was found that the affinityand sensitivity of this receptor were significantlyhigher in endogenous depressed patients thanthose in normal controls.After the electro-acupuncture-treatment (EAT),lymphocyte β—receptor function decreased in patients whoresponded well to EAT,but still did not reachnormal level.(Authors)

  1. Alteration of interaction between astrocytes and neurons in different stages of diabetes: a nuclear magnetic resonance study using [1-(13)C]glucose and [2-(13)C]acetate.

    Wang, Na; Zhao, Liang-Cai; Zheng, Yong-Quan; Dong, Min-Jian; Su, Yongchao; Chen, Wei-Jian; Hu, Zi-Long; Yang, Yun-Jun; Gao, Hong-Chang

    2015-01-01

    Increasing evidence has shown that the brain is a site of diabetic end-organ damage. This study investigates cerebral metabolism and the interactions between astrocytes and neurons at different stages of diabetes to identify the potential pathogenesis of diabetic encephalopathy. [1-(13)C]glucose or [2-(13)C]acetate is infused into 1- and 15-week diabetic rats, the brain extracts of which are analyzed by using (1)H and (13)C magnetic resonance spectroscopy. The (13)C-labeling pattern and enrichment of cerebral metabolites are also investigated. The increased (13)C incorporation in the glutamine, glutamate, and γ-aminobutyric acid carbons from [2-(13)C]acetate suggests that the astrocytic mitochondrial metabolism is enhanced in 1-week diabetic rats. By contrast, the decreased labeling from [1-(13)C]glucose reflected that the neuronal mitochondrial metabolism is impaired. As diabetes developed to 15 weeks, glutamine and glutamate concentrations significantly decreased. The increased labeling of glutamine C4 but unchanged labeling of glutamate C4 from [2-(13)C]acetate suggests decreased astrocyte supply to the neurons. In addition, the enhanced pyruvate recycling pathway manifested by the increased lactate C2 enrichment in 1-week diabetic rats is weakened in 15-week diabetic rats. Our study demonstrates the overall metabolism disturbances, changes in specific metabolic pathways, and interaction between astrocytes and neurons during the onset and development of diabetes. These results contribute to the mechanistic understanding of diabetes pathogenesis and evolution. PMID:25048983

  2. Efficacy of application of vaccine AI H5N1 clade 2.1.3 on Mojosari ducks challenge against AI H5N1 clade 2.3.2 in laboratory conditions

    Indriani R

    2014-03-01

    Full Text Available Influenza virus HPAI H5N1 clade 2.3.2 outbreaks since September 2012 caused high mortality in ducks. Vaccination is one of strategies recommended by government. However, AI H5N1 clade 2.3.2 vaccine not yet available during this research, while AI H5N1 clade 2.1.3 vaccines available in markets. Therefore it was important to do study on efficay of HPAI H5N1 clade 2.1.3. vaccines on duck at laboratory scale. Three groups of Mojosari duck were used in this study, they were 1 group vaccinated with A Vaccine, 1 group vaccinated with B Vaccine, and 1 group as control (not vaccinated. Vacination groups consisted of 9 DOD and control group was consisted of 6 DOD. Vaccination was conducted when the duck at three weeks old of age using single dose recommended by producer. At three weeks later (ducks at 6 weeks old of age all Groups of ducks were challenged with virus HPAI H5N1 clade 2.3.2 at dose 106 EID50/ml by drops intranasaly. Result showed that Group 1 (vaccinated with A Vaccine produced 67% protection (3 out of 9 ducks died, Group 2 (vaccinated with B Vaccine produced 100% protection (non out of 9 ducks died, and Group 3 (control, not vaccinated produce 0% protection (all of 9 ducks died. This study give an alternative of choise to use AI H5N1 Clade 2.1.3 vaccine with high protection when AI H5N1 Clade 2.3.2 vaccine not available in markets to controll high mortality in ducks caused by HPAI H5N1 clade 2.3.2 outbreaks.

  3. Influence of physical properties and chemical composition of sample on formation of aerosol particles generated by nanosecond laser ablation at 213 nm

    Hola, Marketa, E-mail: mhola@sci.muni.c [Department of Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, 611 37 Brno (Czech Republic); Konecna, Veronika [Department of Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, 611 37 Brno (Czech Republic); Mikuska, Pavel [Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic v.v.i., Veveri 97, 602 00 Brno (Czech Republic); Kaiser, Jozef [Institute of Physical Engineering, Faculty of Mechanical Engineering, Brno University of Technology, Technicka 2896/2, 616 69 Brno (Czech Republic); Kanicky, Viktor [Department of Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, 611 37 Brno (Czech Republic)

    2010-01-15

    The influence of sample properties and composition on the size and concentration of aerosol particles generated by nanosecond Nd:YAG laser ablation at 213 nm was investigated for three sets of different materials, each containing five specimens with a similar matrix (Co-cemented carbides with a variable content of W and Co, steel samples with minor differences in elemental content and silica glasses with various colors). The concentration of ablated particles (particle number concentration, PNC) was measured in two size ranges (10-250 nm and 0.25-17 mum) using an optical aerosol spectrometer. The shapes and volumes of the ablation craters were obtained by Scanning Electron Microscopy (SEM) and by an optical profilometer, respectively. Additionally, the structure of the laser-generated particles was studied after their collection on a filter using SEM. The results of particle concentration measurements showed a significant dominance of particles smaller than 250 nm in comparison with larger particles, irrespective of the kind of material. Even if the number of particles larger than 0.25 mum is negligible (up to 0.1%), the volume of large particles that left the ablation cell can reach 50% of the whole particle volume depending on the material. Study of the ablation craters and the laser-generated particles showed a various number of particles produced by different ablation mechanisms (particle splashing or condensation), but the similar character of released particles for all materials was observed by SEM after particle collection on the membrane filter. The created aerosol always consisted of two main structures - spherical particles with diameters from tenths to units of micrometers originally ejected from the molten surface layer and mum-sized 'fibres' composed of primary agglomerates with diameters in the range between tens and hundreds of nanometers. The shape and structure of ablation craters were in good agreement with particle concentration

  4. A novel description of a syndrome consisting of 7q21.3 deletion including DYNC1I1 with preserved DLX5/6 without ectrodactyly: a case report

    Ramos-Zaldívar, Héctor M.; Martínez-Irías, Daniel G.; Espinoza-Moreno, Nelson A.; Napky-Rajo, José S.; Bueso-Aguilar, Tulio A.; Reyes-Perdomo, Karla G.; Montes-Gambarelli, Jimena A.; Euceda, Isis M.; Ponce-Barahona, Aldo F.; Gámez-Fernández, Carlos A.; Moncada-Arita, Wilberg A.; Palomo-Bermúdez, Victoria A.; Jiménez-Faraj, Julia E.; Hernández-Padilla, Amanda G.; Olivera, Denys A.

    2016-01-01

    Background Chromosomal region 7q21.3 comprises approximately 5.2 mega base pairs that include genes DLX5/6, SHFM1, and DYNC1I1 associated with split hand/split foot malformation 1. So far, there are reports of eight families with deletion of DYNC1I1 and preserved DLX5/6 associated with ectrodactyly. From these families, only three patients did not present ectrodactyly and, unlike our patient, no other cases have been described as having craniofacial dysmorphology, mitral valve prolapse, kypho...

  5. 2,1,3-benzothiadiazole-5,6-dicarboxylic imide - A versatile building block for additive- and annealing-free processing of organic solar cells with effi ciencies exceeding 8%

    Nielsen, Christian Bergenstof

    2014-12-15

    A new photoactive polymer comprising benzo[1,2-b:3,4-b′:5,6-d′]trithiophene and 2,1,3-benzothiadiazole-5,6-dicarboxylic imide is reported. The synthetic design allows for alkyl chains to be introduced on both electron-rich and electron-deficient components, which in turn allows for rapid optimization of the alkyl chain substitution pattern. Consequently, the optimized polymer shows a maximum efficiency of 8.3% in organic photovoltaic devices processed in commercially viable fashion without solvent additives, annealing, or device engineering.

  6. 住院糖尿病患者的老年综合征调查%Geriatric syndrome:a survey on 213 elderly inpatients with diabetes mellitus

    王秋梅; 朱鸣雷; 曾平; 康琳; 葛楠; 曲璇; 刘晓红

    2015-01-01

    Objective To investigate the prevalence of geriatric syndrome in the hospitalized elderly with diabetes mellitus. Methods A total of 213 elderly patients at an age of over 65 years with type 2 diabetes mellitus admitted in our department from July 2012 to September 2014 were enrolled in this study. Each subject was assessed for cognitive impairment, mood, sleep problems, falls risk, urinary incontinence, nutrition risk, and impairment of activity of daily living within 48h after admission. Results The cohort was at an age of (74.7±6.6) years, with a duration of diabetes for (12.4±8.9) years (median 10 years), and with 3 to 27 types of comorbidities. They averagely had (3.3±1.7) geriatric syndrome. Sixty percent of them had polypharmacy, and 40%to 50% had impairment in activities of daily living. The prevalence of cognitive impairment, emotional disorder, chronic pain and high risk of falling was 39.0%, 29.0%, 31.9%and 43.0%, respectively. The incidence of falling risk was higher in the patients with longer disease course [54.8%(course>10 years) vs 37.9%(course≤10 years)]. When compared with the well-controlled diabetic patients (HbA1c ≤7.5%), the poor-controlled patients (HbA1c>7.5%) were found to have longer disease course [(17.7±10.5) vs (10.9±7.7) years], higher risk rate of falling (61.7% vs 38.5%), and higher incidences of sleep disorder (51.1% vs 34.9%), but lower incidence of cognitive impairment (27.6% vs 44.8%; all P<0.05). Conclusion The elderly with diabetes mellitus have high risks of cognitive impairment, emotional disorder, chronic pain and falling risks. We need to make comprehensive geriatric assessment and individualized intervention for them.%目的:探讨住院老年糖尿病患者的老年综合征罹患情况。方法对北京协和医院内科老年病房2012年7月至2014年9月收治的213例≥65岁老年糖尿病患者进行老年综合评估,评估患者的认知、情绪、睡眠障碍、跌倒、尿失禁、营养风险和

  7. Report of a consultants' meeting on containment and confinement performance in NPPS with WWER 440/213 and 440/230 reactors

    The reactors WWER 440 model V-230 being operated in several countries of central and eastern Europe were designed in the 1960-ies assuming that the design basis accident can be limited to a break of a pipe of 100 mm diameter fitted with an orifice of 32 mm diameter. This assumption presently judged to be inadequate according to the international safety requirements, was used to determine the Emergency Core Cooling System. For this size of the break, the spray system of the reactor building was designed to prevent uncontrolled releases to the environment. For larger breaks, till full rupture of one of two pressurizer surge lines of 200 mm diameter each, large flaps were designed to open during the initial pressure peak after the accident and release to the environment large amounts of steam escaping from the broken reactor coolant system (RCS). In view of this initial release of radioactive medium and due to its other unsatisfactory features, the building surrounding the WWER 440/230 reactor is not called a containment, but is said to fulfill a confining function, in short it is termed a confinement. The shortcomings of the WWER 440/230 confinements have been long recognized and the newer model of WWER 440 units, called model V-213, has been provided with an improved building of higher leaktightness and higher strength, containing a complicated system of water trays and air traps designed to condense steam and remove a part of the air from the reactor compartments after large break loss of coolant accident (LB LOCA). For such case, the system provides fast pressure decrease in the containment to subatmospheric values and thus prevents any significant releases of radioactivity to the environment. As the main process is that of steam bubble condensation in water, the system is called bubbler condenser containment. The bubbler condenser containment was the answer to the problem of providing the old WWER 440 model 230 units with a reliable containment without excessive

  8. A randomised double-blind clinical trial of two yellow fever vaccines prepared with substrains 17DD and 17D-213/77 in children nine-23 months old

    2015-09-01

    Full Text Available This randomised, double-blind, multicentre study with children nine-23 months old evaluated the immunogenicity of yellow fever (YF vaccines prepared with substrains 17DD and 17D-213/77. YF antibodies were tittered before and 30 or more days after vaccination. Seropositivity and seroconversion were analysed according to the maternal serological status and the collaborating centre. A total of 1,966 children were randomised in the municipalities of the states of Mato Grosso do Sul, Minas Gerais and São Paulo and blood samples were collected from 1,714 mothers. Seropositivity was observed in 78.6% of mothers and 8.9% of children before vaccination. After vaccination, seropositivity rates of 81.9% and 83.2%, seroconversion rates of 84.8% and 85.8% and rates of a four-fold increase over the pre-vaccination titre of 77.6% and 81.8% were observed in the 17D-213/77 and 17DD subgroups, respectively. There was no association with maternal immunity. Among children aged 12 months or older, the seroconversion rates of 69% were associated with concomitant vaccination against measles, mumps and rubella. The data were not conclusive regarding the interference of maternal immunity in the immune response to the YF vaccine, but they suggest interference from other vaccines. The failures in seroconversion after vaccination support the recommendation of a booster dose in children within 10 years of the first dose.

  9. 气相色谱/三重四极杆串联质谱法测定牛奶及奶粉中213种农药多残留%Determination of 213 pesticide residues in milk and milk power by gas chromatography-triple quadrupole tandem mass spectrometry

    王敬; 艾连峰; 马育松; 张海超; 李玮; 于猛

    2015-01-01

    On the basis of the optimization of solid phase extraction adsorbent,eluting solvent types and amounts,a gas chromatography-triple quadrupole tandem mass spectrometry( GC-MS/MS)method was established for the determination of 213 pesticide residues in milk and milk power. The samples were extracted by acetonitrile,cleaned-up with an ENVI-Carb/NH2 solid-phase extractant,and determined by GC-MS/MS using external standard method. The linear ranges were from 10 to 1 000 μg/L for 197 pesticides,from 50 to 1 000 μg/L for the other 16 pesticides with the correlation coefficients higher than 0. 99. The limits of detection( LODs, S/N=3)varied over the range of 0. 03 to 7. 59 μg/kg,and limits of quantification( LOQs,S/N=10)ranged from 0. 10 to 21. 94 μg/kg. The average recoveries in different matrices were in the range of 66. 9%-120. 1% with the relative standard deviations( RSDs ) of 1. 23%-17. 6%. This method is simple,rapid,sensitive and reliable for meeting the requirements for the simul-taneous identification and quantification of the multi-residues in milk and milk power.%在系统优化固相萃取吸附剂填料类型、洗脱溶剂种类及体积的基础上,建立了牛奶和奶粉中213种农药残留的气相色谱-三重四极杆串联质谱( GC-MS/MS)方法。试样用乙腈均质提取,采用石墨化炭黑/氨基柱( ENVI-Carb/NH2)净化后,用 GC-MS/MS多反应离子监测(MRM)模式进行检测,外标法定量。结果表明,197种农药在10~1000μg/L,16种农药在50~1000μg/L范围内线性关系良好,相关系数均大于0.99,方法的检出限( S/N=3)为0.03~7.59μg/kg,定量限( S/N=10)为0.10~21.94μg/kg,平均添加回收率为66.9%~120.1%,相对标准偏差( RSD)为1.23%~17.6%。该方法样品处理简单快速,相比其他多残留分析方法净化效果好,灵敏度和选择性高,适用于日常检测工作。

  10. The contribution of a 9p21.3 variant, a KIF6 variant, and C-reactive protein to predicting risk of myocardial infarction in a prospective study

    Tracy Russell P

    2011-03-01

    Full Text Available Abstract Background Genetic risk factors might improve prediction of coronary events. Several variants at chromosome 9p21.3 have been widely reported to be associated with coronary heart disease (CHD in prospective and case-control studies. A variant of KIF6 (719Arg has also been reported to be associated with increased risk of CHD in large prospective studies, but not in case-control studies. We asked whether the addition of genetic information (the 9p21.3 or KIF6 variants or a well-established non-genetic risk factor (C-reactive protein [CRP] can improve risk prediction by the Framingham Risk Score (FRS in the Cardiovascular Health Study (CHS--a prospective observational study of risk factors for cardiovascular disease among > 5,000 participants aged 65 or older. Methods Improvement of risk prediction was assessed by change in the area under the receiver-operator characteristic curve (AUC and by net reclassification improvement (NRI. Results Among white participants the FRS was improved by addition of KIF6 719Arg carrier status among men as assessed by the AUC (from 0.581 to 0.596, P = 0.03 but not by NRI (NRI = 0.027, P = 0.32. Adding both CRP and 719Arg carrier status to the FRS improved risk prediction by the AUC (0.608, P = 0.02 and NRI (0.093, P = 0.008 in men, but not women (P ≥ 0.24. Conclusions While none of these risk markers individually or in combination improved risk prediction among women, a combination of KIF6 719Arg carrier status and CRP levels modestly improved risk prediction among white men; although this improvement is not significant after multiple-testing correction. These observations should be investigated in other prospective studies.

  11. Preliminary characterisation of new glass reference materials (GSA-1G, GSC-1G, GSD-1G and GSE-1G) by laser ablation-inductively coupled plasma-mass spectrometry using 193 nm, 213 nm and 266 nm wavelengths

    Guillong, M.; Hametner, K.; Reusser, E.; Wilson, S.A.; Gunther, D.

    2005-01-01

    New glass reference materials GSA-1G, GSC-1G, GSD-1G and GSE-1G have been characterised using a prototype solid state laser ablation system capable of producing wavelengths of 193 nm, 213 nm and 266 nm. This system allowed comparison of the effects of different laser wavelengths under nearly identical ablation and ICP operating conditions. The wavelengths 213 nm and 266 nm were also used at higher energy densities to evaluate the influence of energy density on quantitative analysis. In addition, the glass reference materials were analysed using commercially available 266 nm Nd:YAG and 193 nm ArF excimer lasers. Laser ablation analysis was carried out using both single spot and scanning mode ablation. Using laser ablation ICP-MS, concentrations of fifty-eight elements were determined with external calibration to the NIST SRM 610 glass reference material. Instead of applying the more common internal standardisation procedure, the total concentration of all element oxide concentrations was normalised to 100%. Major element concentrations were compared with those determined by electron microprobe. In addition to NIST SRM 610 for external calibration, USGS BCR-2G was used as a more closely matrix-matched reference material in order to compare the effect of matrix-matched and non matrix-matched calibration on quantitative analysis. The results show that the various laser wavelengths and energy densities applied produced similar results, with the exception of scanning mode ablation at 266 nm without matrix-matched calibration where deviations up to 60% from the average were found. However, results acquired using a scanning mode with a matrix-matched calibration agreed with results obtained by spot analysis. The increased abundance of large particles produced when using a scanning ablation mode with NIST SRM 610, is responsible for elemental fractionation effects caused by incomplete vaporisation of large particles in the ICP.

  12. Reflection asymmetry in odd-A and odd-odd actinium nuclei

    Theoretical calculations and measurements indicate that octupole correlations are at a maximum in the ground states of the odd-proton nuclei Ac and Pa. It has been expected that odd-odd nuclei should have even larger amount of octupole-octupole correlations. We have recently made measurements on the structure of 224Ac. Although spin and parity assignments could not be made, two bands starting at 354.1 and 360.0 keV have properties characteristic of reflection asymmetric shape. These two bands have very similar rotational constants and also similar alpha decay rates, which suggest similarity between the wavefunctions of these bands. These signatures provide evidence for octupole correlations in these nuclides

  13. Bestimmung der Ionisationsenergie von Actinium und Ultraspurenanalyse von Plutonium mit resonanter Ionisationsmassenspektrometrie (RIMS)

    Waldek, Achim Marcus

    2001-01-01

    ZusammenfassungDie Resonanzionisationsmassenspektrometrie (RIMS) verbindet hohe Elementselektivität mit guter Nachweiseffizienz. Aufgrund dieser Eigenschaften ist die Methode für Ultraspurenanalyse und Untersuchungen an seltenen oder schwer handhabbaren Elementen gut geeignet. Für RIMS werden neutrale Atome mit monochromatischem Laserlicht ein- oder mehrfach resonant auf energetisch hoch liegende Niveaus angeregt und anschließend durch einen weiteren Laserstrahl oder durch ein elektrisches Fe...

  14. Radium-228 analysis of natural waters by Cherenkov counting of Actinium-228

    The activities of 228Ra in natural waters were determined by the Cherenkov counting of the daughter nuclide 228Ac. The radium was pre-concentrated on MnO2 and the radium purified via ion exchange and, after a 2-day period of incubation to allow for secular equilibrium between the parent-daughter 228Ra(228Ac), the daughter nuclide 228Ac was isolated by ion exchange according to the method of Nour et al. [2004. Radium-228 determination of natural waters via concentration on manganese dioxide and separation using Diphonix ion exchange resin. Appl. Radiat. Isot. 61, 1173-1178]. The Cherenkov photons produced by 228Ac were counted directly without the addition of any scintillation reagents. The optimum Cherenkov counting window, sample volume, and vial type were determined experimentally to achieve optimum Cherenkov photon detection efficiency and lowest background count rates. An optimum detection efficiency of 10.9±0.1% was measured for 228Ac by Cherenkov counting with a very low Cherenkov photon background of 0.317±0.013 cpm. The addition of sodium salicylate into the sample counting vial at a concentration of 0.1 g/mL yielded a more than 3-fold increase in the Cherenkov detection efficiency of 228Ac to 38%. Tests of the Cherenkov counting technique were conducted with several water standards of known activity and the results obtained compared closely with a conventional liquid scintillation counting technique. The advantages and disadvantages of Cherenkov counting compared to liquid scintillation counting methods are discussed. Advantages include much lower Cherenkov background count rates and consequently lower minimal detectable activities for 228Ra and no need for expensive environmentally unfriendly liquid scintillation cocktails. The disadvantages of the Cherenkov counting method include the need to measure 228Ac Cherenkov photon detection efficiency and optimum Cherenkov counting volume, which are not at all required when liquid scintillation analysis is used

  15. Linear free energy relationship applied to trivalent cations with lanthanum and actinium oxide and hydroxide structure

    Linear free energy relationships for trivalent cations with crystalline M2O3 and, M(OH)3 phases of lanthanides and actinides were developed from known thermodynamic properties of the aqueous trivalent cations, modifying the Sverjensky and Molling equation. The linear free energy relationship for trivalent cations is as ΔGf,MvX0=aMvXΔGn,M3+0+bMvX+βMvXrM3+, where the coefficients aMvX, bMvX, and βMvX characterize a particular structural family of MvX, rM3+ is the ionic radius of M3+ cation, ΔGf,MvX0 is the standard Gibbs free energy of formation of MvX and ΔGn,M3+0 is the standard non-solvation free energy of the cation. The coefficients for the oxide family are: aMvX=0.2705, bMvX=-1984.75 (kJ/mol), and βMvX=197.24 (kJ/molnm). The coefficients for the hydroxide family are: aMvX=0.1587, bMvX=-1474.09 (kJ/mol), and βMvX=791.70 (kJ/molnm).

  16. Actinium: A RESTful Runtime Container for Scriptable Internet of Things Applications

    Kovatsch, Matthias; Lanter, Martin; Duquennoy, Simon

    2012-01-01

    Programming Internet of Things (IoT) applications is challenging because developers have to be knowledgeable in various technical domains, from low-power networking, over embedded operating systems, to distributed algorithms. Hence, it will be challenging to find enough experts to provide software for the vast number of expected devices, which must also be scalable and particularly safe due to the connection to the physical world. To remedy this situation, we propose an architecture that pr...

  17. Synthesis of chelating agents for actinium 225 complexation and its application in radioimmunotherapy

    Immunotherapy with radiolabeled antibodies should allow fairly specific targeting of certain cancers. However, iodine 131 may not be the best isotope for tumor therapy because of its limited specific activity, low beta-energy, relatively long half life and strong gamma emission. Another approach to improve therapeutic efficacy is the use of replacement isotopes with better physical properties. Chelator that can hold radio-metals with high stability under physiological conditions are essential to avoid excessive damage to non-target cells; Moreover, the development of new bifunctional chelating agents is essential for this purpose. Accordingly, our efforts have been directed, for several years, to the synthesis of original chelating agents likely to form stable complexes in vivo with the numerous potential candidates for such applications. Therefore, we have developed a new simple and efficient synthesis pathway of 2-(4-iso-thio-cyanate-benzyl)-1,4,7,10,13,16- hexa-aza-cyclo-hexadecane- 1,4,7,10,13,16-hexa-acetic acid, though functionalized on the cycle by a termination allowed coupling to an antibody or any other biological substance such as a hapten. (author)

  18. Purification of selenium from thorium, uranium, radium, actinium and potassium impurities for low background measurements

    A technique of selenium purification from 232Th, 238U, 226,228Ra, 227Ac and 40K was developed. This technique is simple to perform and employs a minimum number of highly pure reagents (bidistilled water, nitric acid). Operations carried out during purification (elution, evaporation) practically exclude losses of the target product (chemical yields of Se > 99%). A test purification of 100 g of selenium was carried out using this technique. The efficiency of this technique was confirmed by low background gamma spectrometry of the purified selenium sample. Distribution coefficients of Th, U, Ra and Ac on DOWEX 50W- x 8 cation-exchange resin at different concentrations of selenium and nitric acid were experimentally determinated. Instrumental neutron activation analysis of bidistilled water, deionized water and nitric acid was performed. (orig.)

  19. Purification of selenium from thorium, uranium, radium, actinium and potassium impurities for low background measurements

    Rakhimov, A.V. [Joint Institute for Nuclear Research (JINR), Dubna (Russian Federation); Uzbek Academy of Sciences, Tashkent (Uzbekistan). Inst. of Nuclear Physics (INP AS RUz); Warot, G. [CEA-CNRS, Modane (France). Laboratoire Souterrain de Modane (LSM); Karaivanov, D.V. [Joint Institute for Nuclear Research (JINR), Dubna (Russian Federation); Institute for Nuclear Research and Nuclear Energy (INRNE), Sofia (Bulgaria); Kochetov, O.I.; Lebedev, N.A.; Filosofov, D.V. [Joint Institute for Nuclear Research (JINR), Dubna (Russian Federation); Mukhamedshina, N.M.; Sadikov, I.I. [Uzbek Academy of Sciences, Tashkent (Uzbekistan). Inst. of Nuclear Physics (INP AS RUz)

    2013-07-01

    A technique of selenium purification from {sup 232}Th, {sup 238}U, {sup 226,228}Ra, {sup 227}Ac and {sup 40}K was developed. This technique is simple to perform and employs a minimum number of highly pure reagents (bidistilled water, nitric acid). Operations carried out during purification (elution, evaporation) practically exclude losses of the target product (chemical yields of Se > 99%). A test purification of 100 g of selenium was carried out using this technique. The efficiency of this technique was confirmed by low background gamma spectrometry of the purified selenium sample. Distribution coefficients of Th, U, Ra and Ac on DOWEX 50W- x 8 cation-exchange resin at different concentrations of selenium and nitric acid were experimentally determinated. Instrumental neutron activation analysis of bidistilled water, deionized water and nitric acid was performed. (orig.)

  20. Reply to “Reply to comments on defining biominerals and organominerals: Direct and indirect indicators of life [Perry et al., Sedimentary Geology, 201, 157–179]” by R.S. Perry and M.A. Sephton: [Sedimentary Geology 213 (2009) 156

    Défarge, Christian; Gautret, Pascale; Reitner, Joachim; Trichet, Jean

    2010-01-01

    International audience This is a reply to R.S. Perry and M.A. Sephton's “Reply to comments on defining biominerals and organominerals: direct and indirect indicators of life [Perry et al., Sedimentary Geology, 201, 157–179]” [Sedimentary Geology 213 (2009) 156].

  1. 汶川地震中国道 G213左侧某典型高山河谷场地地震滑坡响应分析%ANALYSIS OF LANDSLIDE RESPONSES OF A TYPICAL HIGH HILL-RIVER VALLEY FIELD NEAR NATIONAL ROAD G213 DURING WENCHUAN EARTHQUAKE

    杨长卫; 张建经

    2013-01-01

      以国道 G213左侧一处包含河谷地形的高陡边坡为原型,采用新型离散元计算方法——基于连续模型的离散元方法(CDEM),对高烈度地震作用下高陡边坡上的堆积体滑坡由变形累计到破坏滑动的全过程进行模拟,并结合振动台试验结果,对该高陡边坡上堆积体的地震滑坡响应进行研究。研究结果表明,在地震力和重力作用下,堆积体顶部先出现应力集中,造成堆积体沿基岩–堆积体结构面后缘产生变形,进而造成该处出现拉伸、剪切破坏点,之后随着地震动的持续,基岩–堆积体结构面上的剪切破坏点逐渐向堆积体中前部的锁固段扩展,同时伴随着堆积体表面拉伸破坏点的增加,最终造成锁固段发生渐进性破坏,堆积体从剪出口滑出形成滑坡。滑塌发生的时间与地震动峰值加速度到达的时间同步或稍微有所滞后。在高陡边坡地形中,以输入地震波为基准,不论是坡面还是坡体内,不同位置的峰值加速度沿坡高均有所放大,表现为竖向峰值加速度的放大效应>水平峰值加速度的放大效应,坡面峰值加速度的放大效应>坡体内峰值加速度的放大效应。在河谷地形中,以输入波为基准,不论是河床还是河岸两侧的斜坡,不同位置的峰值加速度沿高程均具有不同程度的放大;河谷对加速度放大效应的影响具有一定范围,且在该范围内水平加速度的放大效应>竖向加速度的放大效应,与坡面处加速度的放大效应刚好相反;加速度的放大效应具有一定的方向性,且该方向性与河岸两侧斜坡的坡度有关;加速度放大效应在河谷底部的分布具有不均匀性,距离河岸越近,加速度放大效应越强烈。%  A high steep hill with river valley near National Road G213 is used as a prototype. A full process from initial deformation to sliding of the

  2. 慢性胰腺炎CT影像解剖学分型及其临床意义%CT imaging-anatomy type of total 213 chronic pancreatitis and its clinical significance

    陈盛; 王卫东; 汪华侨

    2011-01-01

    Objective To explore CT imaging-anatomy type of chronic pancreatitis and their clinical significance based on the CT characteristics of chronic pancreatitis. Methods In total 213 cases of chronic pancreatitis patiernts , 156 males and 57 females, with an average age of 48.0±11.5 years old and an average hospital day of 18.4±9.2 days were enrolles in the study.History of bile duct disease and acute pancreatitis were positive in 68 patients (32%) and 36 patients (17%) respectively. Non contrast enhanced and contrast enhanced CT scans were performed (with range from liver to kidney artery level). Results 69 cases (32.4%) showed whole pancreas atrophic. Pancreatic pseudocyst was shown in 3lcases (14.5%). Pancreatic duct occlusion or dilated can be seen in 56 cases ( 26.3%). 18 cases (8.5%) for the mass type , showed local soft tissue samples of pancreatic tumors and 39 cases (18.3%) were mixed. appearance of dilated pancreatic duct or bile duct with pancreatic mass or pseudocyst etc.Conclusion The CT manifestations of chronic pancreatitis can be classified into 5 types: atrophy type, pancreatic pseudocyst type, pancreatic duct occlusion or dilatation type, mass type and mixed hybrid type. The classification had certain significance for the differential diagnosis and the etiological analysis of chronic pancreatitis.%目的 根据慢性胰腺炎CT表现的不同,探讨慢性胰腺炎的CT影像解剖学分型及分型的意义.方法收集1996年1月至2009年1月入住我院经临床或手术证实的213例慢性胰腺炎患者,其中男性156例,女性57例,平均年龄为(48.0±11.5)岁;平均住院天数(18.4±9.2)d;胆系疾病68例(32%),反复发作急性胰腺炎36例(17%).常规肝胆脾胰平扫加增强(肝至肾动脉水平).结果 213例慢性胰腺炎患者中,有69例(32.4%)表现为全胰腺萎缩型,31例(14.5%)表现为假性囊肿型,56例(26.3%)表现为胰管闭塞或扩张,18例(8.5%)表现局限性胰腺

  3. NE-213-scintillator-based neutron detection system for diagnostic measurements of energy spectra for neutrons having energies greater than or equal to 0.8 MeV created during plasma operations at the Princeton Tokamak Fusion Test Reactor

    A system for making diagnostic measurements of the energy spectra of greater than or equal to 0.8-MeV neutrons produced during plasma operations of the Princeton Tokamak Fusion Test Reactor (TFTR) has been fabricated and tested and is presently in operation in the TFTR Test Cell Basement. The system consists of two separate detectors, each made up of cells containing liquid NE-213 scintillator attached permanently to RCA-8850 photomultiplier tubes. Pulses obtained from each photomultiplier system are amplified and electronically analyzed to identify and separate those pulses due to neutron-induced events in the detector from those due to photon-induced events in the detector. Signals from each detector are routed to two separate Analog-to-Digital Converters, and the resulting digitized information, representing: (1) the raw neutron-spectrum data; and (2) the raw photon-spectrum data, are transmited to the CICADA data-acquisition computer system of the TFTR. Software programs have been installed on the CICADA system to analyze the raw data to provide moderate-resolution recreations of the energy spectrum of the neutron and photon fluences incident on the detector during the operation of the TFTR. A complete description of, as well as the operation of, the hardware and software is given in this report

  4. Paleomagnetism of Basaltic Lava Flows in Coreholes ICPP 213, ICPP-214, ICPP-215, and USGS 128 Near the Vadose Zone Research Park, Idaho Nuclear Technology and Engineering Center, Idaho National Engineering and Environmental Laboratory, Idaho

    Champion, Duane E.; Herman, Theodore C.

    2003-01-01

    A paleomagnetic study was conducted on basalt from 41 lava flows represented in about 2,300 ft of core from coreholes ICPP-213, ICPP-214, ICPP-215, and USGS 128. These wells are in the area of the Idaho Nuclear Technology and Engineering Center (INTEC) Vadose Zone Research Park within the Idaho National Engineering and Environmental Laboratory (INEEL). Paleomagnetic measurements were made on 508 samples from the four coreholes, which are compared to each other, and to surface outcrop paleomagnetic data. In general, subhorizontal lines of correlation exist between sediment layers and between basalt layers in the area of the new percolation ponds. Some of the basalt flows and flow sequences are strongly correlative at different depth intervals and represent important stratigraphic unifying elements. Some units pinch out, or thicken or thin even over short separation distances of about 1,500 ft. A more distant correlation of more than 1 mile to corehole USGS 128 is possible for several of the basalt flows, but at greater depth. This is probably due to the broad subsidence of the eastern Snake River Plain centered along its topographic axis located to the south of INEEL. This study shows this most clearly in the oldest portions of the cored sections that have differentially subsided the greatest amount.

  5. Production of CH (A2 Δ) by multi-photon dissociation of (CH3)2CO, CH3NO2, CH2Br2, and CHBr3 at 213 nm

    In order to realize electrostatic Stark deceleration of CH radicals and study cold chemistry, the fifth harmonic of a YAG laser is used to prepare CH (A2Δ) molecules through using the multi-photon dissociation of (CH3)2CO, CH3NO2, CH2Br2, and CHBr3 at ∼ 213 nm. The CH product intensity is measured by using the emission spectrum of CH (A2 Δ → X2 Π). The dependence of fluorescence intensity on laser power is studied, and the probable dissociation channels are analyzed. The relationship between the fluorescence intensity and some parameters, such as the temperature of the beam source, stagnation pressure, and the time delay between the opening of pulse valve and the photolysis laser, are also studied. The influence of three different carrier gases on CH signal intensity is investigated. The vibrational and rotational temperatures of the CH (A2 Δ) product are obtained by comparing experimental data with the simulated ones from the LIFBASE program. (atomic and molecular physics)

  6. Review of the methods used for leak rate measurements for WWER-440/230 confinements and WWER-440/213 containments. A publication of the extrabudgetary programme on the safety of WWER and RBMK nuclear power plants

    Program was initiated by IAEA in 1990 with the aim to assist the countries of Central and Eastern Europe and former Soviet Union in evaluating the safety of their first generation WWER-440/230 nuclear power plants. The main objectives were: to identify major design and operational safety issues; to establish international consensus on priorities for safety improvements; and to provide assistance in the review of the competence and and adequacy of safety improvement programs. The scope was extended in 1992 ro include RBMK, WWER-440/312 and WWER-1000 plants in operation and under construction. One of the characteristic features od confinements in WWER-440/213 reactors is their comparatively high leakage rate, ranging from several hundred per cent per day upwards, which is lower than those of WWER-440/230 but in both types of containments the task of leakage rate determination presents complex problems which do not appear in western type units. This report contains the requirements for containment leak rate testing and for integral test in the countries operating WWER reactors and comparison with the requirements in force in USA, Germany and France. It describes the leak rate testing principles and leak rate extrapolation methods, discusses in detail the differences in leakage rate definitions due to significant leakages in WWER containments and explains their consequences. The procedures for structural integrity tests of WWER-440 containments are described and recommendations given

  7. 4-(8-Quinolyl)amino-7-nitro-2,1,3-benzoxadiazole as a new selective and sensitive fluorescent and colorimetric pH probe with dual-responsive ranges in aqueous solutions

    Li, Xutian; Zhang, Min; Liang, Haipeng; Huang, Zhaowei; Tang, Jiang; Chen, Zhi; Yang, Liting; Ma, Li-Jun; Wang, Yuhai; Xu, Baiping

    2016-01-01

    Fluorescent and colorimetric pH probe possess many advantages including rapid response time, nondestructive testing, and excellent pH sensitivity. However, they usually cannot be utilized simultaneously in both acidic and basic pH ranges. In this study, a new selective and sensitive fluorescent and colorimetric pH probe, 4-(8- quinolyl)amino-7-nitro-2,1,3-benzoxadiazole (1), was designated and synthesized. The optical probe exhibited dual-responsive pH ranges to both acidic and basic aqueous solutions. When the solution pH was gradually increased from 8.5 to 13.3, the absorption spectra of 1 showed an obvious hyperchromicity, accompanied with a red shift of the absorption band at 340 nm, a blue shift of the absorption band at 482 nm, and a distinct color change from orange to violet pink to yellow. Within the pH range from 2.2 to 0.2, the fluorescent spectra of 1 showed a "turn-on" response signal to solution pH. In order to understand the response mechanism of the probe to solution pH, the probe molecule was split into two parts, 8-aminoquinoline (2) and 4-amino-7- nitro-benzofurazan (3). UV-vis absorption and fluorescent experiments of 2 and 3 indicated that both are sensitive optical pH probes. Furthermore, the NMR experiment of 1 was explored in basic and acidic conditions. The results indicated that the colorimetric responses of 1 to pH under basic condition should be attributed to the deprotonation of the imino group on the quinolyl ring, and the fluorescent recognition of 1 to pH under acidic condition was probably due to the protonation of the nitrogen atoms from the benzofurazan and quinolyl rings.

  8. Electrosynthesis and characterization of a donor–acceptor type electrochromic material from poly(4,7-dicarbazol-9-yl-2,1,3-benzothiadia-zole) and its application in electrochromic devices

    A donor–acceptor type monomer 4,7-dicarbazol-9-yl-2,1,3-benzothiadiazole (CBTD) was synthesized by modified Ullmann coupling reaction and its corresponding polymer (PCBTD) was obtained via electrochemical polymerization. Characterizations of the resulting polymer PCBTD were performed by cyclic voltammetry, UV–vis spectroscopy, Fourier transform infrared spectroscopy and scanning electron microscopy. The polymer displayed multiple colors changed from yellow to yellowish green, green, and turquoise blue with the applied potential. The UV–vis spectra depicted that polymer film have two absorption bands as expected for this type of donor–acceptor polymer, at 320 and 467 nm, which attributed to the π–π* transition and intrachain charge transfer transition, respectively. Further, electrochemical and optical band gaps were obtained by using their oxidation and reduction onset potentials and absorption edges, respectively. The optical contrasts of the PCBTD film were found to be 28.1% at 420 nm and 49.9% at 800 nm in the visible region. The dual type electrochromic device constructed utilizing PCBTD and poly(3,4-ethylenedioxythiophene) showed a maximum contrast (ΔT %) of 49.4% at 620 nm with response time of 0.6 s and exhibited high coloration efficiency (1728 cm2 C−1) and good stability. These properties make the polymer a suitable material for electrochromic applications. - Highlights: ► A donor–acceptor carbazole based monomer and its polymer were synthesized. ► The polymer film showed good electrochromic properties and high optical contrast. ► An electrochromic device based on the polymer was also constructed and characterized

  9. A Genome Wide Study of Copy Number Variation Associated with Nasopharyngeal Carcinoma in Malaysian Chinese Identifies CNVs at 11q14.3 and 6p21.3 as Candidate Loci.

    Joyce Siew Yong Low

    Full Text Available Nasopharyngeal carcinoma (NPC is a neoplasm of the epithelial lining of the nasopharynx. Despite various reports linking genomic variants to NPC predisposition, very few reports were done on copy number variations (CNV. CNV is an inherent structural variation that has been found to be involved in cancer predisposition.A discovery cohort of Malaysian Chinese descent (NPC patients, n = 140; Healthy controls, n = 256 were genotyped using Illumina® HumanOmniExpress BeadChip. PennCNV and cnvPartition calling algorithms were applied for CNV calling. Taqman CNV assays and digital PCR were used to validate CNV calls and replicate candidate copy number variant region (CNVR associations in a follow-up Malaysian Chinese (NPC cases, n = 465; and Healthy controls, n = 677 and Malay cohort (NPC cases, n = 114; Healthy controls, n = 124.Six putative CNVRs overlapping GRM5, MICA/HCP5/HCG26, LILRB3/LILRA6, DPY19L2, RNase3/RNase2 and GOLPH3 genes were jointly identified by PennCNV and cnvPartition. CNVs overlapping GRM5 and MICA/HCP5/HCG26 were subjected to further validation by Taqman CNV assays and digital PCR. Combined analysis in Malaysian Chinese cohort revealed a strong association at CNVR on chromosome 11q14.3 (Pcombined = 1.54x10-5; odds ratio (OR = 7.27; 95% CI = 2.96-17.88 overlapping GRM5 and a suggestive association at CNVR on chromosome 6p21.3 (Pcombined = 1.29x10-3; OR = 4.21; 95% CI = 1.75-10.11 overlapping MICA/HCP5/HCG26 genes.Our results demonstrated the association of CNVs towards NPC susceptibility, implicating a possible role of CNVs in NPC development.

  10. 50 CFR 21.3 - Definitions.

    2010-10-01

    ... environment that is intensively manipulated by man for the purpose of producing raptors of selected species... entering or leaving the controlled environment. General characteristics of captivity may include, but are... for pursuit of wild game, and hunting wild game with raptors. Falconry includes the taking of...

  11. 49 CFR 213.337 - Defective rails.

    2010-10-01

    ... crystalline center or nucleus inside the head from which it spreads outward as a smooth, bright, or dark... a smooth, bright, or dark surface progressing until substantially at a right angle to the length of... through it. A crack or rust streak may show under the head close to the web or pieces may be split off...

  12. Fungal Planet description sheets: 154–213

    Crous, P.W.; Wingfield, M.J.; Guarro, J.; Cheewangkoon, R.; Bank, van der M.; Swart, W.J.; Stchigel, A.M.; Cano-Lira, J.F.; Roux, J.; Madrid, H.; Damm, U.; Wood, A.R.; Shuttleworth, L.A.; Hodges, C.S.; Munster, M.; Jesús Yáñez-Morales, de M.; Zúñiga-Estrada, L.; Cruywagen, E.M.; Hoog, de G.S.; Silvera, C.; Najafzadeh, J.; Davison, E.M.; Davison, P.J.N.; Barrett, M.D.; Barrett, R.L.; Manamgoda, D.S.; Minnis, A.M.; Kleczewski, N.M.; Flory, S.L.; Castlebury, L.A.; Clay, K.; Hyde, K.D.; Maússe-Sitoe, S.N.D.; Chen, S.; Lechat, C.; Hairaud, M.; Lesage-Meessen, L.; Pawlowska, J.; Wilk, M.; Sliwinska-Wyrzychowska, A.; Metrak, M.; Wrzosek, M.; Pavlic-Zupanc, D.; Maleme, H.M.; Slippers, B.; Mac Cormack, W.P.; Archuby, D.I.; Grünwald, N.J.; Tellería, M.T.; Dueñas, M.; Martín, M.P.; Marincowitz, S.; Beer, de Z.W.; Perez, C.A.; Gené, J.; Marin-Felix, Y.; Groenewald, J.Z.

    2013-01-01

    Novel species of microfungi described in the present study include the following from South Africa: Camarosporium aloes, Phaeococcomyces aloes and Phoma aloes from Aloe, C. psoraleae, Diaporthe psoraleae and D. psoraleae-pinnatae from Psoralea, Colletotrichum euphorbiae from Euphorbia, Coniothyrium

  13. SU-B-213-01: Introduction

    Starkschall, G. [UT MD Anderson Cancer Center (United States)

    2015-06-15

    The North American medical physics community validates the education received by medical physicists and the clinical qualifications for medical physicists through accreditation of educational programs and certification of medical physicists. Medical physics educational programs (graduate education and residency education) are accredited by the Commission on Accreditation of Medical Physics Education Programs (CAMPEP), whereas medical physicists are certified by several organizations, the most familiar of which is the American Board of Radiology (ABR). In order for an educational program to become accredited or a medical physicist to become certified, the applicant must meet certain specified standards set by the appropriate organization. In this Symposium, representatives from both CAMPEP and the ABR will describe the process by which standards are established as well as the process by which qualifications of candidates for accreditation or certification are shown to be compliant with these standards. The Symposium will conclude with a panel discussion. Learning Objectives: Recognize the difference between accreditation of an educational program and certification of an individual Identify the two organizations primarily responsible for these tasks Describe the development of educational standards Describe the process by which examination questions are developed GS is Executive Secretary of CAMPEP.

  14. 49 CFR 180.213 - Requalification markings.

    2010-10-01

    ... provided that— (A) Permission is obtained from the cylinder owner; (B) The minimum wall thickness is... the letters “AE” for acoustic emission or “UE” for ultrasonic examination. (9) For designation of...

  15. 47 CFR 213.7 - Policies.

    2010-10-01

    ... OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL GOVERNMENT AND PUBLIC... classification above shall have no precedence over others within the same classification, except where, within the same classification, they cannot be handled simultaneously. Then, they shall be handled in...

  16. SU-B-213-01: Introduction

    The North American medical physics community validates the education received by medical physicists and the clinical qualifications for medical physicists through accreditation of educational programs and certification of medical physicists. Medical physics educational programs (graduate education and residency education) are accredited by the Commission on Accreditation of Medical Physics Education Programs (CAMPEP), whereas medical physicists are certified by several organizations, the most familiar of which is the American Board of Radiology (ABR). In order for an educational program to become accredited or a medical physicist to become certified, the applicant must meet certain specified standards set by the appropriate organization. In this Symposium, representatives from both CAMPEP and the ABR will describe the process by which standards are established as well as the process by which qualifications of candidates for accreditation or certification are shown to be compliant with these standards. The Symposium will conclude with a panel discussion. Learning Objectives: Recognize the difference between accreditation of an educational program and certification of an individual Identify the two organizations primarily responsible for these tasks Describe the development of educational standards Describe the process by which examination questions are developed GS is Executive Secretary of CAMPEP

  17. Fungal Planet description sheets: 154-213.

    Crous, P W; Wingfield, M J; Guarro, J; Cheewangkoon, R; van der Bank, M; Swart, W J; Stchigel, A M; Cano-Lira, J F; Roux, J; Madrid, H; Damm, U; Wood, A R; Shuttleworth, L A; Hodges, C S; Munster, M; de Jesús Yáñez-Morales, M; Zúñiga-Estrada, L; Cruywagen, E M; de Hoog, G S; Silvera, C; Najafzadeh, J; Davison, E M; Davison, P J N; Barrett, M D; Barrett, R L; Manamgoda, D S; Minnis, A M; Kleczewski, N M; Flory, S L; Castlebury, L A; Clay, K; Hyde, K D; Maússe-Sitoe, S N D; Chen, Shuaifei; Lechat, C; Hairaud, M; Lesage-Meessen, L; Pawłowska, J; Wilk, M; Sliwińska-Wyrzychowska, A; Mętrak, M; Wrzosek, M; Pavlic-Zupanc, D; Maleme, H M; Slippers, B; Mac Cormack, W P; Archuby, D I; Grünwald, N J; Tellería, M T; Dueñas, M; Martín, M P; Marincowitz, S; de Beer, Z W; Perez, C A; Gené, J; Marin-Felix, Y; Groenewald, J Z

    2013-12-01

    Novel species of microfungi described in the present study include the following from South Africa: Camarosporium aloes, Phaeococcomyces aloes and Phoma aloes from Aloe, C. psoraleae, Diaporthe psoraleae and D. psoraleae-pinnatae from Psoralea, Colletotrichum euphorbiae from Euphorbia, Coniothyrium prosopidis and Peyronellaea prosopidis from Prosopis, Diaporthe cassines from Cassine, D. diospyricola from Diospyros, Diaporthe maytenicola from Maytenus, Harknessia proteae from Protea, Neofusicoccum ursorum and N. cryptoaustrale from Eucalyptus, Ochrocladosporium adansoniae from Adansonia, Pilidium pseudoconcavum from Greyia radlkoferi, Stagonospora pseudopaludosa from Phragmites and Toxicocladosporium ficiniae from Ficinia. Several species were also described from Thailand, namely: Chaetopsina pini and C. pinicola from Pinus spp., Myrmecridium thailandicum from reed litter, Passalora pseudotithoniae from Tithonia, Pallidocercospora ventilago from Ventilago, Pyricularia bothriochloae from Bothriochloa and Sphaerulina rhododendricola from Rhododendron. Novelties from Spain include Cladophialophora multiseptata, Knufia tsunedae and Pleuroascus rectipilus from soil and Cyphellophora catalaunica from river sediments. Species from the USA include Bipolaris drechsleri from Microstegium, Calonectria blephiliae from Blephilia, Kellermania macrospora (epitype) and K. pseudoyuccigena from Yucca. Three new species are described from Mexico, namely Neophaeosphaeria agaves and K. agaves from Agave and Phytophthora ipomoeae from Ipomoea. Other African species include Calonectria mossambicensis from Eucalyptus (Mozambique), Harzia cameroonensis from an unknown creeper (Cameroon), Mastigosporella anisophylleae from Anisophyllea (Zambia) and Teratosphaeria terminaliae from Terminalia (Zimbabwe). Species from Europe include Auxarthron longisporum from forest soil (Portugal), Discosia pseudoartocreas from Tilia (Austria), Paraconiothyrium polonense and P. lycopodinum from Lycopodium (Poland) and Stachybotrys oleronensis from Iris (France). Two species of Chrysosporium are described from Antarctica, namely C. magnasporum and C. oceanitesii. Finally, Licea xanthospora is described from Australia, Hypochnicium huinayensis from Chile and Custingophora blanchettei from Uruguay. Novel genera of Ascomycetes include Neomycosphaerella from Pseudopentameris macrantha (South Africa), and Paramycosphaerella from Brachystegia sp. (Zimbabwe). Novel hyphomycete genera include Pseudocatenomycopsis from Rothmannia (Zambia), Neopseudocercospora from Terminalia (Zambia) and Neodeightoniella from Phragmites (South Africa), while Dimorphiopsis from Brachystegia (Zambia) represents a novel coelomycetous genus. Furthermore, Alanphillipsia is introduced as a new genus in the Botryosphaeriaceae with four species, A. aloes, A. aloeigena and A. aloetica from Aloe spp. and A. euphorbiae from Euphorbia sp. (South Africa). A new combination is also proposed for Brachysporium torulosum (Deightoniella black tip of banana) as Corynespora torulosa. Morphological and culture characteristics along with ITS DNA barcodes are provided for all taxa. PMID:24761043

  18. Fungal Planet description sheets: 154-213

    Crous, P.W.; Wingfield, M.J.; Guarro, J; Cheewangkoon, R.; Bank, van der, M.; Swart, W J; Stchigel, A. M.; Cano-Lira, J.F.; Roux, J.; Madrid, H.; Damm, U.; Wood, A. R.; Shuttleworth, L.A.; Hodges, C.S.; Munster, M.

    2013-01-01

    Novel species of microfungi described in the present study include the following from South Africa: Camarosporium aloes, Phaeococcomyces aloes and Phoma aloes from Aloe, C. psoraleae, Diaporthe psoraleae and D. psoraleae-pinnatae from Psoralea, Colletotrichum euphorbiae from Euphorbia, Coniothyrium prosopidis and Peyronellaea prosopidis from Prosopis, Diaporthe cassines from Cassine, D. diospyricola from Diospyros, Diaporthe maytenicola from Maytenus, Harknessia proteae from Protea, Neofusico...

  19. Effects of Electromagnetic Fields on Fish and Invertebrates: Task 2.1.3: Effects on Aquatic Organisms - Fiscal Year 2011 Progress Report - Environmental Effects of Marine and Hydrokinetic Energy

    Woodruff, Dana L.; Schultz, Irvin R.; Marshall, Kathryn E.; Ward, Jeffrey A.; Cullinan, Valerie I.

    2012-05-01

    This fiscal year (FY) 2011 progress report (Task 2.1.3 Effects on Aquatic Organisms, Subtask 2.3.1.1 Electromagnetic Fields) describes studies conducted by PNNL as part of the DOE Wind and Water Power Program to examine the potential effects of electromagnetic fields (EMF) from marine and hydrokinetic devices on aquatic organisms, including freshwater and marine fish and marine invertebrates. In this report, we provide a description of the methods and results of experiments conducted in FY 2010-FY 2011 to evaluate potential responses of selected aquatic organisms. Preliminary EMF laboratory experiments during FY 2010 and 2011 entailed exposures with representative fish and invertebrate species including juvenile coho salmon (Oncorhynchus kisutch), Atlantic halibut (Hippoglossus hippoglossus), California halibut (Paralicthys californicus), rainbow trout (Oncorhynchus mykiss), and Dungeness crab (Metacarcinus magister). These species were selected for their ecological, commercial, and/or recreational importance, as well as their potential to encounter an MHK device or transmission cable during part or all of their life cycle. Based on previous studies, acute effects such as mortality were not expected to occur from EMF exposures. Therefore, our measurement endpoints focused on behavioral responses (e.g., detection of EMF, interference with feeding behavior, avoidance or attraction to EMF), developmental changes (i.e., growth and survival from egg or larval stage to juvenile), and exposure markers indicative of physiological responses to stress. EMF intensities during the various tests ranged from 0.1 to 3 millitesla, representing a range of upper bounding conditions reported in the literature. Experiments to date have shown there is little evidence to indicate distinct or extreme behavioral responses in the presence of elevated EMF for the species tested. Several developmental and physiological responses were observed in the fish exposures, although most were not

  20. RISKAUDIT Report no. 7, Vol. 2: Safety evaluation of VVER 440/213 and VVER 1000/320 reactors in Rovno NPP Units 1, 2 and 3. Final Report by AIB-Vincotte Nuclear, CIEMAT, ANPA, GRS, IPSN, AEA-T

    NONE

    1994-07-15

    The Riskaudit 7 report has been made by a group of independent experts from Western European countries representing technical organizations specialized in the support of regulatory bodies of those countries. It represents a preliminary estimation of the Ukrainian WWER B 213 and B 320 reactors, based on the example of Rovno NPP, analysed with a Western practice. The second part of the document covers the following aspects of the report: accident analysis; systems analysis; plant operation; operating experience feedback; radio protection and health; probabilistic safety assessment; summary and future plans.

  1. RISKAUDIT Report no. 7, Vol. 2: Safety evaluation of VVER 440/213 and VVER 1000/320 reactors in Rovno NPP Units 1, 2 and 3. Final Report by AIB-Vincotte Nuclear, CIEMAT, ANPA, GRS, IPSN, AEA-T

    The Riskaudit 7 report has been made by a group of independent experts from Western European countries representing technical organizations specialized in the support of regulatory bodies of those countries. It represents a preliminary estimation of the Ukrainian WWER B 213 and B 320 reactors, based on the example of Rovno NPP, analysed with a Western practice. The second part of the document covers the following aspects of the report: accident analysis; systems analysis; plant operation; operating experience feedback; radio protection and health; probabilistic safety assessment; summary and future plans

  2. RISKAUDIT Report no. 7, Vol. 1: Safety evaluation of VVER 440/213 and VVER 1000/320 reactors in Rovno NPP Units 1, 2 and 3. Final Report by AIB-Vincotte Nuclear, CIEMAT, ANPA, GRS, IPSN, AEA-T

    The Riskaudit 7 report has been made by a group of independent experts from Western European countries representing technical organizations specialized in the support of regulatory bodies of those countries. It represents a preliminary estimation of the Ukrainian WWER B 213 and B 320 reactors, based on the example of Rovno NPP, analysed with a Western practice. The first part of the document covers the following aspects of the report: core design and fuel management; pressurized components; electrical supply; instrumentation and control; containment; internal events; site conditions and external events

  3. RISKAUDIT Report no. 7, Vol. 1: Safety evaluation of VVER 440/213 and VVER 1000/320 reactors in Rovno NPP Units 1, 2 and 3. Final Report by AIB-Vincotte Nuclear, CIEMAT, ANPA, GRS, IPSN, AEA-T

    NONE

    1994-07-15

    The Riskaudit 7 report has been made by a group of independent experts from Western European countries representing technical organizations specialized in the support of regulatory bodies of those countries. It represents a preliminary estimation of the Ukrainian WWER B 213 and B 320 reactors, based on the example of Rovno NPP, analysed with a Western practice. The first part of the document covers the following aspects of the report: core design and fuel management; pressurized components; electrical supply; instrumentation and control; containment; internal events; site conditions and external events.

  4. Purification of radium-226 for the manufacturing of actinium-225 in a cyclotron for alpha-immunotherapy; Radium-Aufreinigung zur Herstellung von Actinium-225 am Zyklotron fuer die Alpha-Immuntherapie

    Marx, Sebastian Markus

    2014-09-23

    The thesis describes the development of methods for the purification of Ra-226. The objective was to obtain the radionuclide in the quality that is needed to be used as starting material in the manufacturing process for Ac-225 via proton-irradiated Ra-226. The radionuclide has been gained efficiently out of huge excesses of impurities. The high purity of the obtained radium affords its use as staring material in a pharmaceutical manufacturing process.

  5. Tantalacyclobutane complexes containing the potentially C,N,N'-Coordinating ligand [C6H4(CH2N(Me)CH2CH2NMe2)-2]- (CNN) and their reactivity with carbon monoxide and tert-butyl isocyanide. X-ray molecular structures of [Ta{CH2CH(Me)CH2-1,3} (CNN)(O-t-Bu)2], [Ta{CH2CH(Ph)CH2-1,3} (CNN) (O-t-Bu)2], [Ta{C(O)((CH2)3-1,3)} (CNN) (Ot-Bu)2], and [Ta{C(=N-t-Bu)CH2CH(Ph)CH2-1,4} (CNN (O-t-Bu)2

    van Koten, G; Rietveld, M.H.P.; Hagen, H.; van de Water, L.; Grove, D.M.; Kooijman, H.; Veldman, N; Spek, A.L.

    1997-01-01

    From the reactions of the unsubstituted tantalacyclobutane complex [Ta{(CH2)3-1,3}(CNN)(O-t-Bu)2] (CNN = [C6H4(CH2N(Me)CH2CH2NMe2)-2]-) (1) with propene and styrene have been isolated in good yields the substituted tantalacyclobutane complexes [Ta{CH2CH(Me)CH2-1,3}(CNN)(O-t-Bu)2] (2) and [Ta{CH2CH(Ph)CH2-1,3}(CNN)(O-t-Bu)2] (3), respectively. The X-ray molecular structures of 2 and 3 show them to be seven-coordinate pentagonal bipyramidal species in which the meridional ligation comprises C,N...

  6. Rolls-Royce successful modernization of safety-critical Instrumentation and Control (I and C) equipment at the Dukovany VVER 440/213 Nuclear Power Plant, based on SPINLINE 3 platform

    Rolls-Royce has provided on-time delivery of a substantial safety-critical I and C overhaul for four Nuclear reactors operated by Czech Republic utility, CEZ a.s. This nine-year project is considered to be one of the largest I and C modernization projects in the world. The Dukovany VVER 440 I and C modernization project and its key success factors are profiled in this paper. The project is in the final stages with the last unit to be completed in 2009. Beginning in September 2000, the project is in compliance with the initial schedule. Rolls-Royce has been designing and manufacturing I and C solutions dedicated to the implementation of safety and safety-related functions in nuclear power plants (NPPs) for more than 30 years. Though the early solutions were non-software-based, since 1984 software-based solutions for safety I and C functions have been deployed in operating NPPs across France and 15 other countries. The Rolls-Royce platform is suitable for implementation of safety I and C functions in new NPPs, as well as in the modernization of safety equipment in existing plants. CEZ a.s. is a major electricity supplier for the national grid. At Dukovany, CEZ a.s. operates four units of VVER-440/213-type reactors producing one quarter of CEZ a.s. electricity production. The first of these units was connected to the grid in 1985. Since the year 2000, the nine-year modernization program has been underway at Dukovany, at a cost of more than 200 million Euros. The equipment replacement was implemented during regular, planned outages of the original equipment and systems. After an international bidding phase, CEZ a.s. awarded a contract to Skoda JS for general engineering and project management. Individual subcontracts were then signed between Skoda JS and a consortium between Rolls-Royce and Areva for modernization of the safety systems, including the Reactor Protection System (RPS), the Reactor Control System (RCS), and the Post-Accident Monitoring System (PAMS). Two

  7. Rolls-Royce successful modernization of safety-critical Instrumentation and Control (I and C) equipment at the Dukovany VVER 440/213 Nuclear Power Plant, based on SPINLINE 3 platform

    Rebreyend, P.; Burel, J.P. [Rolls-Royce Civil Nuclear SAS (France); Spoc, J. [Skoda JS (Czech republic); Karasek, A. [CEZ a.s.(Czech republic)

    2010-07-01

    Rolls-Royce has provided on-time delivery of a substantial safety-critical I and C overhaul for four Nuclear reactors operated by Czech Republic utility, CEZ a.s. This nine-year project is considered to be one of the largest I and C modernization projects in the world. The Dukovany VVER 440 I and C modernization project and its key success factors are profiled in this paper. The project is in the final stages with the last unit to be completed in 2009. Beginning in September 2000, the project is in compliance with the initial schedule. Rolls-Royce has been designing and manufacturing I and C solutions dedicated to the implementation of safety and safety-related functions in nuclear power plants (NPPs) for more than 30 years. Though the early solutions were non-software-based, since 1984 software-based solutions for safety I and C functions have been deployed in operating NPPs across France and 15 other countries. The Rolls-Royce platform is suitable for implementation of safety I and C functions in new NPPs, as well as in the modernization of safety equipment in existing plants. CEZ a.s. is a major electricity supplier for the national grid. At Dukovany, CEZ a.s. operates four units of VVER-440/213-type reactors producing one quarter of CEZ a.s. electricity production. The first of these units was connected to the grid in 1985. Since the year 2000, the nine-year modernization program has been underway at Dukovany, at a cost of more than 200 million Euros. The equipment replacement was implemented during regular, planned outages of the original equipment and systems. After an international bidding phase, CEZ a.s. awarded a contract to Skoda JS for general engineering and project management. Individual subcontracts were then signed between Skoda JS and a consortium between Rolls-Royce and Areva for modernization of the safety systems, including the Reactor Protection System (RPS), the Reactor Control System (RCS), and the Post-Accident Monitoring System (PAMS). Two

  8. Purification of radium-226 for the manufacturing of actinium-225 in a cyclotron for alpha-immunotherapy

    The thesis describes the development of methods for the purification of Ra-226. The objective was to obtain the radionuclide in the quality that is needed to be used as starting material in the manufacturing process for Ac-225 via proton-irradiated Ra-226. The radionuclide has been gained efficiently out of huge excesses of impurities. The high purity of the obtained radium affords its use as staring material in a pharmaceutical manufacturing process.

  9. Distribution of trace elements in land plants and botanical taxonomy with special reference to rare earth elements and actinium

    Distribution profiles of trace elements in land plants were studied by neutron activation analysis and radioactivity measurements without activation. Number of botanical samples analyzed were more than three thousand in which more than three hundred botanical species were included. New accumulator plants of Co, Cr, Zn, Cd, rare earth elements, Ac, U, etc., were found. Capabilities of accumulating trace elements can be related to the botanical taxonomy. Discussions are given from view points of inorganic chemistry as well as from botanical physiology

  10. 49 CFR 571.213 - Standard No. 213; Child restraint systems.

    2010-10-01

    ... center SORL (as illustrated in figure 1B of this standard); and (2) Neither knee pivot point shall pass... restraint system, neither knee pivot point shall, at any time during the dynamic test, pass through a... pivot point, measured along a horizontal line that passes through the knee pivot point and is...

  11. Discovery of difference in the diffuse scattering from nonstoichiometric Ca0.87La0.13F2.13 and Ca0.92Er0.08F2.08 crystals with different types of clusters of structural defects

    A new approach to application of diffuse scattering (DS) for studying the defect structure of crystals on laboratory radiation sources is implemented. The basic principles of this approach are as follows: (i) choice of crystals with a high concentration of structural defects (highly nonstoichiometric Ca1−xRxF2+x phases), (ii) application of intermeasurement minimization method (experimental comparison) to select a weak desired DS signal from a superposition of signals of different nature, and (iii) choice of the basic model proceeding from the reliable information provided by accurate analysis of the contribution of structural defects to Bragg reflections. Significantly different DS diffraction patterns have been recorded for Ca0.87La0.13F2.13 and Ca0.92Er0.08F2.08 crystals, characterized by different types of structural-defect clusters, determined from the Bragg diffraction data. Experiments performed at 90–100 K proved that DS is caused by clusters with stable atomic configurations rather than cooperative thermal atomic vibrations. A set of methods is proposed which can efficiently be used in diagnostics of nanomaterials

  12. Calculation of the response of the WWER-440/213 reactor core to an accident associated with main steam header break by means of the ATHLET system code in combination with with the three-dimensional reactor dynamics code DYN3D

    A survey is given of the behavior of the reactor core during an accident associated with main steam header break. This problem is a benchmark exercise for the verification of thermohydraulic system codes connected with three-dimensional hexagonal reactor dynamics codes. The accident is initiated by a symmetrical break of the main steam header at the end of the 1st fuelling cyclein the hot shutdown condition with the control rod groups fully inserted except for group 4. The calculations were performed by using the externally coupled codes ATHLET Mod. 1.1 Cycle C and DYN3DH1.1/M3. The two codes are described at their external coupling is outlined. The standard WWER-440/213 input deck of the ATHLET code was used. The tresults of steady-state calculations as well as burnup calculations are given. The analysis of the transient process is aimed at the time behavior of safety related parameters in selected components of the primary and secondary circuits as well as at the global parameters of the reactor core and behavior in selected fuel assemblies

  13. {2-[1-(3-Methoxycarbonylmethyl-1H-indol-2-yl)-1-methyl-ethyl]-1H-indol-3-yl}-acetic Acid Methyl Ester Inhibited Hepatocellular Carcinoma Growth in Bel-7402 Cells and Its Resistant Variants by Activation of NOX4 and SIRT3.

    Li, Ye; Wang, Wenjing; Xu, Xiaoxue; Sun, Shiyue; Xu, Xiaoyu; Qu, Xian-jun

    2015-01-01

    {2-[1-(3-Methoxycarbonylmethyl-1H-indol-2-yl)-1-methyl-ethyl]-1H-indol-3-yl}-acetic acid methyl ester (MIAM) is a novel indole compound, which possessed high efficacy against many cancers xenografted in mice without obvious toxicity. In this study, we aimed to investigate the effects of MIAM on human hepatocellular carcinoma (HCC) Bel-7402 cells and its resistant variants Bel-7402/5FU. MIAM inhibited the growth of HCC more potent in Bel-7402/5FU cells than its parent cells. MIAM increased cellular reactive oxygen species (ROS) levels, induced cell apoptosis, and arrested cell cycle in G0/G1 phase. MIAM might exert its action on Bel-7402/5FU cells through activation of NADPH oxidase 4 (NOX4)/p22(phox), Sirtuin3 (SIRT3)/SOD2, and SIRT3/p53/p21(Waf1/Cip) pathways. MIAM might inhibit HCC growth through the modulation of SIRT3. When SIRT3 was silenced, the inhibitory effect of MIAM on Bel-7402/5FU was lowered, showing the characteristic of resistance against MIAM, whereas Bel-7402/5FU cells with high expression of SIRT3 by SIRT3 adenovirus infection demonstrated the high sensitivity to MIAM. These results suggested that MIAM might exert its action against Bel-7402/5FU growth through upregulation of SIRT3. We suggested that MIAM might be a promising candidate compound which could develop as a potent anticancer agent targeting NOX4 and SIRT3 activation. PMID:25961022

  14. {2-[1-(3-Methoxycarbonylmethyl-1H-indol-2-yl-1-methyl-ethyl]-1H-indol-3-yl}-acetic Acid Methyl Ester Inhibited Hepatocellular Carcinoma Growth in Bel-7402 Cells and Its Resistant Variants by Activation of NOX4 and SIRT3

    Ye Li

    2015-01-01

    Full Text Available {2-[1-(3-Methoxycarbonylmethyl-1H-indol-2-yl-1-methyl-ethyl]-1H-indol-3-yl}-acetic acid methyl ester (MIAM is a novel indole compound, which possessed high efficacy against many cancers xenografted in mice without obvious toxicity. In this study, we aimed to investigate the effects of MIAM on human hepatocellular carcinoma (HCC Bel-7402 cells and its resistant variants Bel-7402/5FU. MIAM inhibited the growth of HCC more potent in Bel-7402/5FU cells than its parent cells. MIAM increased cellular reactive oxygen species (ROS levels, induced cell apoptosis, and arrested cell cycle in G0/G1 phase. MIAM might exert its action on Bel-7402/5FU cells through activation of NADPH oxidase 4 (NOX4/p22, Sirtuin3 (SIRT3/SOD2, and SIRT3/p53/p21 pathways. MIAM might inhibit HCC growth through the modulation of SIRT3. When SIRT3 was silenced, the inhibitory effect of MIAM on Bel-7402/5FU was lowered, showing the characteristic of resistance against MIAM, whereas Bel-7402/5FU cells with high expression of SIRT3 by SIRT3 adenovirus infection demonstrated the high sensitivity to MIAM. These results suggested that MIAM might exert its action against Bel-7402/5FU growth through upregulation of SIRT3. We suggested that MIAM might be a promising candidate compound which could develop as a potent anticancer agent targeting NOX4 and SIRT3 activation.

  15. Determination of l-tryptophan and l-kynurenine derivatized with (R)-4-(3-isothiocyanatopyrrolidin-1-yl)-7-(N,N-dimethylaminosulfonyl)-2,1,3-benzoxadiazole by LC-MS/MS on a triazole-bonded column and their quantification in human serum.

    Takahashi, Shuuhei; Iizuka, Hideaki; Kuwabara, Ryousuke; Naito, Yoko; Sakamoto, Tatsuya; Miyagi, Aya; Onozato, Mayu; Ichiba, Hideaki; Fukushima, Takeshi

    2016-09-01

    The concentrations of l-tryptophan (Trp) and the metabolite l-kynurenine (KYN) can be used to evaluate the in-vivo activity of indoleamine 2,3-dioxygenase (IDO) and tryptophan 2,3-dioxygenase (TDO). As such, a novel method involving derivatization of l-Trp and l-KYN with (R)-4-(3-isothiocyanatopyrrolidin-1-yl)-7-(N,N-dimethylaminosulfonyl)-2,1,3-benzoxadiazole (DBD-PyNCS) and separation by high-performance liquid chromatography (HPLC) with tandem mass spectrometric (MS/MS) detection on a triazole-bonded column (Cosmosil HILIC®) was developed to determine their concentrations. The optimized mobile phase, CH3 CN/10 mm ammonium formate in H2 O (pH 5.0) (90:10, v/v) eluted isocratically, resulted in satisfactory separation and MS/MS detection of the analytes. The detection limits of l-Trp and l-KYN were approximately 50 and 4.0 pm, respectively. The column temperature affected the retention behaviour of the Trp and KYN derivatives, with increased column temperatures leading to increased capacity factors; positive enthalpy changes were revealed by van't Hoff plot analyses. Using the proposed LC-MS/MS method, l-Trp and l-KYN were successfully determined in 10 μL human serum using 1-methyl-l-Trp as an internal standard. The precision and recovery of l-Trp were in the ranges 2.85-9.29 and 95.8-113%, respectively, while those of l-KYN were 2.51-16.0 and 80.8-98.2%, respectively. The proposed LC-MS/MS method will be useful for evaluating the in vivo activity of IDO or TDO. Copyright © 2016 John Wiley & Sons, Ltd. PMID:26910189

  16. 34 CFR 685.213 - Total and permanent disability discharge.

    2010-07-01

    ... discharged if the borrower becomes totally and permanently disabled, as defined in 34 CFR 682.200(b), and... permanently disabled as described in paragraph (1) of the definition of that term in 34 CFR 682.200(b), the... of that term in 34 CFR 682.200(b), the veteran's loan discharge application is processed...

  17. Bis[2-(1,3-dioxoisoindolin-2-ylethyl] phthalate

    Kai Yang

    2010-02-01

    Full Text Available The title compound, C28H20N2O8, was synthesized by the reaction of isobenzofuran-1,3-dione and 2-aminoethanol in a one-pot reaction. The benzene and five-membered rings are slightly twisted to each other, making dihedral angles of 2.77 (9 and 1.77 (10°. The rings of the phthalimide groups make dihedral angle of 57.64 (7 and 83.46 (7° with the central benzene ring. Weak C—H...O, C—H...π and π–π [centroid–centroid distance = 3.446 (1 and 3.599 (1 Å] interactions reinforce the cohesion of the crystal.

  18. 38 CFR 3.213 - Change of status affecting entitlement.

    2010-07-01

    ... forth the month and year of the child's death, marriage, or discontinuance of school attendance. A.... A statement by the veteran setting forth the month and year of death of a spouse, child, or... setting forth the month and year of remarriage and any change of name. (An award for a child or...

  19. 26 CFR 1.213-1 - Medical, dental, etc., expenses.

    2010-04-01

    ... income under section 105 (c) or (d) (relating to amounts received under accident and health plans) and.... Thus, payments for the following are payments for medical care: hospital services, nursing services... personal or family considerations and not because he requires medical or nursing attention. In such...

  20. 49 CFR 213.333 - Automated vehicle inspection systems.

    2010-10-01

    ... inspections to continuously compare loaded track gage to unloaded gage under a known loading condition. The... with a minimum cut-off frequency of 25 Hz. The sample rate for wheel force data shall be at least 250... feet away from the contact point of wheels carrying a vertical load of no less than 10,000 pounds...

  1. 46 CFR 109.213 - Emergency training and drills.

    2010-10-01

    ... instruction using the on board training aids. Additionally, members of the crew or industrial personnel.... Abandonment training material must be on board each unit. The training material must consist either of a... to each person on board the unit. If audiovisual training aids are used, they must be...

  2. 40 CFR 21.3 - Submission of applications.

    2010-07-01

    ... for the publicly owned treatment works shall be included noting that the sewer construction is... 201 of the Act (see 35 CFR part 917). (iv) If the requirement results from a State order, regulation... for a publicly-owned treatment works, the municipal permit number shall be included along with...

  3. Dicty_cDB: AFA213 [Dicty_cDB

    Full Text Available te sequence, segment 1/2. 60 1e-08 4 BM493927 |BM493927.1 NXLV_071_F02_F NXLV (Nsf Xylem Late wood Vertical)... Pinus taeda cDNA clone NXLV_071_F02 5', mRNA sequence. 50 5e-08 2 BQ634402 |BQ634402.1 NXRV068_E06_F NXRV (Nsf Xylem Root wood Verti...cal) Pinus taeda cDNA clone NXRV068_E06 5', mRNA sequence. 50 6e-08 2 BI416760 |BI4

  4. 49 CFR 213.110 - Gage restraint measurement systems.

    2010-10-01

    ...). c=Coefficient of friction between rail/tie which is assigned a nominal value of (0.4). V=Actual... correlation between measurements made on the ground and those recorded by the instrumentation with respect to... training program shall address— (1) Basic GRMS procedures; (2) Interpretation and handling of...

  5. 20 CFR 410.213 - Duration of entitlement; child.

    2010-04-01

    ... first occurs: (1) The child dies; (2) The child marries; (3) The child attains age 18 and, (i) Is not... the month in which he attains age 18; (4) If the child's entitlement is based on his status as a... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Duration of entitlement; child....

  6. 25 CFR 213.33 - Diligence and prevention of waste.

    2010-04-01

    ... prevention of waste of oil or gas developed on the land, or the entrance of water through wells drilled by... prevention of waste. The lessee shall exercise diligence in drilling and operating wells for oil and gas on... the same and to shut off effectually all water from the oil or gas-bearing strata; not drill any...

  7. External hazards in PSA program for VVER 440/213

    The basic PSA study for the Dukovany NPP was completed in 1995. Since then a Living PSA program has come into force. All the PSA related activities are undertaken under the umbrella called Living PSA. They include support of risk management, data collection and information transfer, maintenance and improvement of PSA models. To support regulatory decision making the full scope PSA is under development. External hazards identified are: earthquake initiators, aircraft crushes and extreme rainfalls. Seismic PSA is not scheduled for near future, but team is being build up and a test example of seismic event impact is being modelled for trial evaluation

  8. 2-(1,3-Benzothiazol-2-yliminomethyl-2-naphthol

    Khadija O. Badahdaha

    2009-04-01

    Full Text Available In the title molecule, C18H12N2OS, the dihedral angle between the two fused-ring systems is 7.2 (1°. The hydroxy group forms an intramolecular hydrogen bond with the imino group.

  9. 14 CFR 67.213 - General medical condition.

    2010-01-01

    ... established medical history or clinical diagnosis of diabetes mellitus that requires insulin or any other... that the Federal Air Surgeon, based on the case history and appropriate, qualified medical judgment... the Federal Air Surgeon, based on the case history and appropriate, qualified medical...

  10. Dicty_cDB: SLB213 [Dicty_cDB

    Full Text Available noimidazole-succinocarbo... 130 9e-48 (A7NKB4) RecName: Full=Phosphoribosylaminoimid...no... 117 3e-47 (A9WK38) RecName: Full=Phosphoribosylaminoimidazole-succinocarbo... 115 3e-47 (A9B302) RecNam...e: Full=Phosphoribosylaminoimidazole-succinocarbo... 120 2e-46 BC048051_1( BC048051 |pid...azole-succinocarbo... 128 2e-47 BC041276_1( BC041276 |pid:none) Xenopus laevis phosphoribosylami...:none) Danio rerio phosphoribosylaminoimi... 114 6e-46 BT074778_1( BT074778 |pid:none)

  11. SU-B-213-07: Panel Discussion

    Starkschall, G. [UT MD Anderson Cancer Center (United States)

    2015-06-15

    The North American medical physics community validates the education received by medical physicists and the clinical qualifications for medical physicists through accreditation of educational programs and certification of medical physicists. Medical physics educational programs (graduate education and residency education) are accredited by the Commission on Accreditation of Medical Physics Education Programs (CAMPEP), whereas medical physicists are certified by several organizations, the most familiar of which is the American Board of Radiology (ABR). In order for an educational program to become accredited or a medical physicist to become certified, the applicant must meet certain specified standards set by the appropriate organization. In this Symposium, representatives from both CAMPEP and the ABR will describe the process by which standards are established as well as the process by which qualifications of candidates for accreditation or certification are shown to be compliant with these standards. The Symposium will conclude with a panel discussion. Learning Objectives: Recognize the difference between accreditation of an educational program and certification of an individual Identify the two organizations primarily responsible for these tasks Describe the development of educational standards Describe the process by which examination questions are developed GS is Executive Secretary of CAMPEP.

  12. SU-B-213-02: Development of CAMPEP Standards

    Beckham, W. [BC Cancer Agency-Vancouver Island Centre (Canada)

    2015-06-15

    The North American medical physics community validates the education received by medical physicists and the clinical qualifications for medical physicists through accreditation of educational programs and certification of medical physicists. Medical physics educational programs (graduate education and residency education) are accredited by the Commission on Accreditation of Medical Physics Education Programs (CAMPEP), whereas medical physicists are certified by several organizations, the most familiar of which is the American Board of Radiology (ABR). In order for an educational program to become accredited or a medical physicist to become certified, the applicant must meet certain specified standards set by the appropriate organization. In this Symposium, representatives from both CAMPEP and the ABR will describe the process by which standards are established as well as the process by which qualifications of candidates for accreditation or certification are shown to be compliant with these standards. The Symposium will conclude with a panel discussion. Learning Objectives: Recognize the difference between accreditation of an educational program and certification of an individual Identify the two organizations primarily responsible for these tasks Describe the development of educational standards Describe the process by which examination questions are developed GS is Executive Secretary of CAMPEP.

  13. 50 CFR 226.213 - Critical habitat for Johnson's seagrass.

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Critical habitat for Johnson's seagrass... Critical habitat for Johnson's seagrass. Critical habitat is designated to include substrate and water in... Johnson's seagrass. (a) A portion of the Indian River, Florida, north of Sebastian Inlet Channel,...

  14. 76 FR 213 - National Environmental Policy Act Implementing Procedures

    2011-01-03

    ... systems; small-scale renewable energy research and development and pilot projects; solar photovoltaic... Energy 10 CFR Part 1021 National Environmental Policy Act Implementing Procedures; Proposed Rule #0;#0;Federal Register / Vol. 76 , No. 1 / Monday, January 3, 2011 / Proposed Rules#0;#0; ] DEPARTMENT OF...

  15. 34 CFR 668.213 - Economically disadvantaged appeals.

    2010-07-01

    ... selected under paragraph (c)(2) of this section, have an expected family contribution, as defined in 34 CFR 690.2, that is equal to or less than the largest expected family contribution that would allow a... Human Services poverty guidelines for the size of the student's family unit. (2) The students who...

  16. 24 CFR 213.256 - Premiums; insurance upon completion.

    2010-04-01

    ...; insurance upon completion. (a) Management and Sales Types and Investor Sponsored Projects. (1) Where the... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Premiums; insurance upon completion... HOUSING AND URBAN DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND...

  17. 24 CFR 213.266a - Insurance fund obligations.

    2010-04-01

    ... obligation either of the Cooperative Management Housing Insurance Fund or of the General Insurance Fund. The... Management Housing Insurance Fund, shall also be the obligation of the General Insurance Fund. ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Insurance fund obligations....

  18. Stabilised electromagnetic levitation at 2-13 MHz

    Danley, T. J.; Schiffman, R. A.; Weber, J. K. R.; Krishnan, S.; Rey, C. A.; Bruno, P. A.; Nordine, P. C.

    1991-01-01

    SEL, the Stabilised Electromagnetic Levitator, has been developed to exploit the unique design opportunities available in containerless microgravity experiments. Efficiency and versatility are obtained with multiple coils driven by individual broadband amplifiers whose phase and frequency are controlled. The heating and positioning fields are decoupled. Specimen translation, spin, and for liquids, shape may be adjusted. An open coil structure provides access for optical and diagnostic probes. Results of experiments with a prototype device are discussed. Levitating and heating materials on earth were demonstrated at frequencies up to 13 MHz.

  19. 27 CFR 40.213 - Tobacco products labeled for export.

    2010-04-01

    ... of the product. For the purposes of this section, “repackaging” shall mean the removal of the tobacco product from its original package bearing the export marks and placement of the product in a new...

  20. SU-B-213-03: Evaluation of Graduate Programs

    The North American medical physics community validates the education received by medical physicists and the clinical qualifications for medical physicists through accreditation of educational programs and certification of medical physicists. Medical physics educational programs (graduate education and residency education) are accredited by the Commission on Accreditation of Medical Physics Education Programs (CAMPEP), whereas medical physicists are certified by several organizations, the most familiar of which is the American Board of Radiology (ABR). In order for an educational program to become accredited or a medical physicist to become certified, the applicant must meet certain specified standards set by the appropriate organization. In this Symposium, representatives from both CAMPEP and the ABR will describe the process by which standards are established as well as the process by which qualifications of candidates for accreditation or certification are shown to be compliant with these standards. The Symposium will conclude with a panel discussion. Learning Objectives: Recognize the difference between accreditation of an educational program and certification of an individual Identify the two organizations primarily responsible for these tasks Describe the development of educational standards Describe the process by which examination questions are developed GS is Executive Secretary of CAMPEP

  1. SU-B-213-04: Evaluation of Residency Programs

    The North American medical physics community validates the education received by medical physicists and the clinical qualifications for medical physicists through accreditation of educational programs and certification of medical physicists. Medical physics educational programs (graduate education and residency education) are accredited by the Commission on Accreditation of Medical Physics Education Programs (CAMPEP), whereas medical physicists are certified by several organizations, the most familiar of which is the American Board of Radiology (ABR). In order for an educational program to become accredited or a medical physicist to become certified, the applicant must meet certain specified standards set by the appropriate organization. In this Symposium, representatives from both CAMPEP and the ABR will describe the process by which standards are established as well as the process by which qualifications of candidates for accreditation or certification are shown to be compliant with these standards. The Symposium will conclude with a panel discussion. Learning Objectives: Recognize the difference between accreditation of an educational program and certification of an individual Identify the two organizations primarily responsible for these tasks Describe the development of educational standards Describe the process by which examination questions are developed GS is Executive Secretary of CAMPEP

  2. SU-B-213-07: Panel Discussion

    The North American medical physics community validates the education received by medical physicists and the clinical qualifications for medical physicists through accreditation of educational programs and certification of medical physicists. Medical physics educational programs (graduate education and residency education) are accredited by the Commission on Accreditation of Medical Physics Education Programs (CAMPEP), whereas medical physicists are certified by several organizations, the most familiar of which is the American Board of Radiology (ABR). In order for an educational program to become accredited or a medical physicist to become certified, the applicant must meet certain specified standards set by the appropriate organization. In this Symposium, representatives from both CAMPEP and the ABR will describe the process by which standards are established as well as the process by which qualifications of candidates for accreditation or certification are shown to be compliant with these standards. The Symposium will conclude with a panel discussion. Learning Objectives: Recognize the difference between accreditation of an educational program and certification of an individual Identify the two organizations primarily responsible for these tasks Describe the development of educational standards Describe the process by which examination questions are developed GS is Executive Secretary of CAMPEP

  3. SU-B-213-02: Development of CAMPEP Standards

    The North American medical physics community validates the education received by medical physicists and the clinical qualifications for medical physicists through accreditation of educational programs and certification of medical physicists. Medical physics educational programs (graduate education and residency education) are accredited by the Commission on Accreditation of Medical Physics Education Programs (CAMPEP), whereas medical physicists are certified by several organizations, the most familiar of which is the American Board of Radiology (ABR). In order for an educational program to become accredited or a medical physicist to become certified, the applicant must meet certain specified standards set by the appropriate organization. In this Symposium, representatives from both CAMPEP and the ABR will describe the process by which standards are established as well as the process by which qualifications of candidates for accreditation or certification are shown to be compliant with these standards. The Symposium will conclude with a panel discussion. Learning Objectives: Recognize the difference between accreditation of an educational program and certification of an individual Identify the two organizations primarily responsible for these tasks Describe the development of educational standards Describe the process by which examination questions are developed GS is Executive Secretary of CAMPEP

  4. 5 CFR 213.3202 - Entire executive civil service.

    2010-01-01

    ... use their training authority in 5 U.S.C. chapter 41 and 5 CFR part 410 to pay all or part of training... use their training authority in 5 U.S.C. chapter 41 and 5 CFR part 410 to pay all or part of training... previous Federal appointment (e.g. fellowships and similar programs in accordance with 5 CFR...

  5. Dicty_cDB: VFI213 [Dicty_cDB

    Full Text Available S12 Capsicum annuum cDNA, mRNA sequence. 54 0.004 1 AW928683 |AW928683.1 EST337471 tomato flower buds 8 mm to pre-anthesis, Cornell...04 1 BF187220 |BF187220.1 EST443507 potato stolon, Cornell University Solanum tuberosum cDNA clone cSTA39D17... 5' sequence, mRNA sequence. 54 0.004 1 BE473010 |BE473010.1 EST417863 potato stolon, Cornell

  6. TRASH TO TREASURE: CONVERTING COLD WAR LEGACY WASTE INTO WEAPONS AGAINST CANCER

    As part of its commitment to clean up Cold War legacy sites, the U.S. Department of Energy (DOE) has initiated an exciting and unique project to dispose of its inventory of uranium-233 (233U) stored at Oak Ridge National Laboratory (ORNL), and extract isotopes that show great promise in the treatment of deadly cancers. In addition to increasing the supply of potentially useful medical isotopes, the project will rid DOE of a nuclear concern and cut surveillance and security costs. For more than 30 years, DOE's ORNL has stored over 1,200 containers of fissile 233U, originally produced for several defense-related projects, including a pilot study that looked at using 233U as a commercial reactor fuel. This uranium, designated as special nuclear material, requires expensive security, safety, and environmental controls. It has been stored at an ORNL facility, Building 3019A, that dates back to the Manhattan Project. Down-blending the material to a safer form, rather than continuing to store it, will eliminate a $15 million a year financial liability for the DOE and increase the supply of medical isotopes by 5,700 percent. During the down-blending process, thorium-229 (229Th) will be extracted. The thorium will then be used to extract actinium-225 (225Ac), which will ultimately supply its progeny, bismuth-213 (213Bi), for on-going cancer research. The research includes Phase II clinical trials for the treatment of acute myelogenous leukemia at Sloan-Kettering Memorial Cancer Center in New York, as well as other serious cancers of the lungs, pancreas, and kidneys using a technique known as alpha-particle radioimmunotherapy. Alpha-particle radioimmunotherapy is based on the emission of alpha particles by radionuclides. 213Bi is attached to a monoclonal antibody that targets specific cells. The bismuth then delivers a high-powered but short-range radiation dose, effectively killing the cancerous cells but sparing the surrounding tissue. Production of the actinium and

  7. TRASH TO TREASURE: CONVERTING COLD WAR LEGACY WASTE INTO WEAPONS AGAINST CANCER

    Nicholas, R.G.; Lacy, N.H.; Butz, T.R.; Brandon, N.E.

    2004-10-06

    As part of its commitment to clean up Cold War legacy sites, the U.S. Department of Energy (DOE) has initiated an exciting and unique project to dispose of its inventory of uranium-233 (233U) stored at Oak Ridge National Laboratory (ORNL), and extract isotopes that show great promise in the treatment of deadly cancers. In addition to increasing the supply of potentially useful medical isotopes, the project will rid DOE of a nuclear concern and cut surveillance and security costs. For more than 30 years, DOE's ORNL has stored over 1,200 containers of fissile 233U, originally produced for several defense-related projects, including a pilot study that looked at using 233U as a commercial reactor fuel. This uranium, designated as special nuclear material, requires expensive security, safety, and environmental controls. It has been stored at an ORNL facility, Building 3019A, that dates back to the Manhattan Project. Down-blending the material to a safer form, rather than continuing to store it, will eliminate a $15 million a year financial liability for the DOE and increase the supply of medical isotopes by 5,700 percent. During the down-blending process, thorium-229 (229Th) will be extracted. The thorium will then be used to extract actinium-225 (225Ac), which will ultimately supply its progeny, bismuth-213 (213Bi), for on-going cancer research. The research includes Phase II clinical trials for the treatment of acute myelogenous leukemia at Sloan-Kettering Memorial Cancer Center in New York, as well as other serious cancers of the lungs, pancreas, and kidneys using a technique known as alpha-particle radioimmunotherapy. Alpha-particle radioimmunotherapy is based on the emission of alpha particles by radionuclides. 213Bi is attached to a monoclonal antibody that targets specific cells. The bismuth then delivers a high-powered but short-range radiation dose, effectively killing the cancerous cells but sparing the surrounding tissue. Production of the actinium and

  8. Study on laser atomic spectroscopy

    Laser atomic spectroscopic study on actinium element has been performed in many areas of spectroscopy. The study on characteristic of atomic vapor has been proceeded for copper atom and the spatial density distribution of copper vapor is measured. This experimental data has been compared with the theoretically calculated data. In spectroscopic experiment, the first and second excited states for actinium element are identified and the most efficient ionization scheme for actinium element is identified. In addition, the corrosion problem for filament material due to the heating of the actinium element has been studied. (Author)

  9. Quantitative analysis of microstructure deformation in creep fenomena of ferritic SA-213 T22 and austenitic SA-213 TP304H material

    Mulyana, Cukup; Taufik, Ahmad; Gunawan, Agus Yodi; Siregar, Rustam Efendi

    2013-09-01

    The failure of critical component of fossil fired power plant that operated in creep range (high stress, high temperature and in the long term) depends on its microstructure characteristics. Ferritic low carbon steel (2.25Cr-1Mo) and Austenitic stainless alloy (18Cr-8Ni) are used as a boiler tube in the secondary superheater outlet header to deliver steam before entering the turbin. The tube failure is occurred in a form of rupture, resulting trip that disrupts the continuity of the electrical generation. The research in quantification of the microstructure deformation has been done in predicting the remaining life of the tube through interrupted accelerated creep test. For Austenitic Stainless Alloy (18Cr-8Ni), creep test was done in 550°C with the stress 424.5 MPa and for Ferritic Low Carbon Steel (2.25Cr-1Mo) in 570°C with the stress 189 MPa. The interrupted accelerated creep test was done by stopping the observation in condition 60%, 70%, 80% and 90% of remaining life, the creep test fracture was done before. Then the micro hardness test, photo micro, SEM and EDS were obtained from those samples. Refer to ASTM E122, microstructure parameters were calculated. The results indicated that there are a consistency of decreasing their grain diameters, increasing their grain size numbers, micro hardness, and the length of crack or void number per unit area with the decreasing of remaining life. While morphology of grain (stated in parameter α=LV/LH) relatively constant for austenitic. However, for ferritic the change of morphology revealed significantly. Fracture mode propagation of ferritic material is growth with voids transgranular and intergranular crack, and for austenitic material the fracture growth with intergranular creep fracture void and wedge crack. In this research, it was proposed a formulation of mathematical model for creep behavior corresponding their curve fitting resulted for the primary, secondary and tertiary in accelerated creep test. In addition, it was also developed a new method for predicting the remaining life using quantification of microstructure and using expansion of parameter Larson Miller from Taylor series for critical component in high temperature in industry. It was found that the proposed method was easier to be applied in field with the results more accurate then Larson Miller Method.

  10. Tumor immunotargeting using innovative radionuclides.

    Kraeber-Bodéré, Françoise; Rousseau, Caroline; Bodet-Milin, Caroline; Mathieu, Cédric; Guérard, François; Frampas, Eric; Carlier, Thomas; Chouin, Nicolas; Haddad, Ferid; Chatal, Jean-François; Faivre-Chauvet, Alain; Chérel, Michel; Barbet, Jacques

    2015-01-01

    This paper reviews some aspects and recent developments in the use of antibodies to target radionuclides for tumor imaging and therapy. While radiolabeled antibodies have been considered for many years in this context, only a few have reached the level of routine clinical use. However, alternative radionuclides, with more appropriate physical properties, such as lutetium-177 or copper-67, as well as alpha-emitting radionuclides, including astatine-211, bismuth-213, actinium-225, and others are currently reviving hopes in cancer treatments, both in hematological diseases and solid tumors. At the same time, PET imaging, with short-lived radionuclides, such as gallium-68, fluorine-18 or copper-64, or long half-life ones, particularly iodine-124 and zirconium-89 now offers new perspectives in immuno-specific phenotype tumor imaging. New antibody analogues and pretargeting strategies have also considerably improved the performances of tumor immunotargeting and completely renewed the interest in these approaches for imaging and therapy by providing theranostics, companion diagnostics and news tools to make personalized medicine a reality. PMID:25679452

  11. Tumor Immunotargeting Using Innovative Radionuclides

    Françoise Kraeber-Bodéré

    2015-02-01

    Full Text Available This paper reviews some aspects and recent developments in the use of antibodies to target radionuclides for tumor imaging and therapy. While radiolabeled antibodies have been considered for many years in this context, only a few have reached the level of routine clinical use. However, alternative radionuclides, with more appropriate physical properties, such as lutetium-177 or copper-67, as well as alpha-emitting radionuclides, including astatine-211, bismuth-213, actinium-225, and others are currently reviving hopes in cancer treatments, both in hematological diseases and solid tumors. At the same time, PET imaging, with short-lived radionuclides, such as gallium-68, fluorine-18 or copper-64, or long half-life ones, particularly iodine-124 and zirconium-89 now offers new perspectives in immuno-specific phenotype tumor imaging. New antibody analogues and pretargeting strategies have also considerably improved the performances of tumor immunotargeting and completely renewed the interest in these approaches for imaging and therapy by providing theranostics, companion diagnostics and news tools to make personalized medicine a reality.

  12. Removal of actinium from europium for the determination of specific radioactivity of ultra low-level Eu-152 in a sample exposed to atomic-bomb neutrons in Nagasaki

    Measurements of specific radioactivities of residual neutron-induced radionuclides such as 152Eu and 60Co have been carried out to check the validity of a series of computer calculations employed for the atomic-bomb neutron dosimetry in Hiroshima (exposed to uranium bomb) and Nagasaki (exposed to plutonium bomb). The use of these nuclides for atomic-bomb neutron dosimetry, however, has been limited by the following difficulties: (1) today, these radionuclides are found only at extremely low concentrations in materials exposed to the atomic-bomb and (2) the neutrons that induced these radionuclides were thermal and epithermal, while the neutron dose received in Hiroshima and Nagasaki is attributable to fast neutrons. In order to overcome the first difficulty, we established a chemical procedure to extract Eu and Co from materials exposed to the atomic-bomb, and the chemical procedure has been successful for the materials exposed to atomic-bomb within 1400 m in slant distance from the explosion point. As for Nagasaki, materials exposed in the distances farther than 1200 m have never been subjected to the measurement of residual neutron-induced radionuclides. In this work, determination of specific radioactivity of 152Eu (half-life: 13.542 y) in a sample exposed to Nagasaki atomic-bomb at a distant place from the explosion point was undertaken. But, because of radioactive decay during this ∝60 years since 1945 and long distance from the explosion point, the present specific radioactivity of 152Eu in the sample is extremely low (estimated to be ∝3 x 10-4 Bq-152Eu/mg-Eu), and a serious problem is interferences from daughters of 227Ac (half-life: 21.8 y) in the measurement of ultra low-level radioactivity of 152Eu. Hence, our chemical procedure to obtain Eu-enriched counting source should be improved, and much attention is being denoted to removal of Ac from Eu. (orig.)

  13. Alpha Emitting Radionuclides and Radiopharmaceuticals for Therapy

    Today, cancer treatments mainly rely on surgery or external beam radiation to remove or destroy bulky tumors. Chemotherapy is given when tumours cannot be removed or when dissemination is suspected. However, these approaches cannot permanently treat all cancers and relapse occurs in up to 50% of the patients’ population. Radioimmunotherapy (RIT) and peptide receptor radionuclide therapy (PRRT) are effective against some disseminated and metastatic diseases, although they are rarely curative. Most preclinical and clinical developments in this field have involved electron-emitting radionuclides, particularly iodine-131, yttrium-90 and lutetium-177. The large range of the electrons emitted by these radionuclides reduces their efficacy against very small tumour cell clusters or isolated tumour cells present in residual disease and in many haematological tumours (leukaemia, myeloma). The range of alpha particles in biological tissues is very short, less than 0.1 mm, which makes alpha emitters theoretically ideal for treatment of such isolated tumour cells or micro-clusters of malignant cells. Thus, over the last decade, a growing interest for the use of alpha-emitting radionuclides has emerged. Research on targeted alpha therapy (TAT) began years ago in Nantes through cooperation between Subatech, a nuclear physics laboratory, CRCNA, a cancer research centre with a nuclear oncology team and ITU (Karlsruhe, Germany). CD138 was demonstrated as a potential target antigen for Multiple Myeloma, which is a target of huge clinical interest particularly suited for TAT because of the disseminated nature of the disease consisting primarily of isolated cells and small clusters of tumour cells mainly localized in the bone marrow. Thus anti-CD138 antibodies were labelled with bismuth-213 from actinium-225/bismuth-213 generators provided by ITU and used to target multiple myeloma cells. In vitro studies showed cell cycle arrest, synergism with chemotherapy and very little induction

  14. SU-D-213-01: Transparent Photon Detector For The Online Monitoring Of IMRT Beams

    Purpose: An innovative Transparent Detector for Radiotherapy (TraDeRa) has been developed. The detector aims at real-time monitoring of modulated beam ahead of the patient during delivery sessions, with a field cover up to 40×40 cm 2. Methods: TraDeRa consists in a pixelated matrix of ionization chambers with a patented electrodes design. An in-house designed specific integrated circuit allows to extract the signal and provides a real-time map of beam intensity and shape, at the linac pulse-scale. The measurements under irradiation are made with a 6 MV clinical X-Ray beam. Dose calculations are performed with the Monte Carlo code PENELOPE, modeling the full accelerator head and the TraDeRa detector. Results: A 2 % attenuation of the beam was measured in the presence of TraDeRa and the PENELOPE dosimetric study showed no significant modification of the photon beam properties. TraDeRa detects error leaf position as small as 1 mm compared to a reference field, for both static and modulated fields. In addition, measurements are accurate over a large dynamic range from low intensity signals, as inter-leaves leaks, to very high intensities as obtained on the medical line of the European Synchrotron Radiation Facility. The detector is fully operational for conventional and high dose rate beams as FFF modes (up to 2400 MU/min). Conclusion: The current version of TraDeRa shows promising results for IMRT quality assurance (QA), allowing pulse-scale monitoring of the beam and high sensitivity for errors detection. The attenuation is small enough not to hinder the irradiation while keeping the beam upstream of the patient under constant control. A final prototype under development will include 1600 independent electrodes, half of them with a high resolution centered on the beam axis. This compact detector provides an independent set of measurements for a better QA. Funding support : This work was supported by the LABEX PRIMES (ANR-11-LABX-0063) of Universite de Lyon, within the program “Investissements d’Avenir” (ANR-11-IDEX-0007) operated by the French National Research Agency (ANR) and within the project “INSPIRA” operated by the OSEO institution

  15. 49 CFR 40.213 - What training requirements must STTs and BATs meet?

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false What training requirements must STTs and BATs meet... requirements must STTs and BATs meet? To be permitted to act as a BAT or STT in the DOT alcohol testing program...). (1) Qualification training must be in accordance with the DOT Model BAT or STT Course, as...

  16. Calibration of ground-based lidar instrument WLS7-213

    Gómez Arranz, Paula; Courtney, Michael

    This report presents the result of the lidar calibration performed for the given WLS7 Windcube at DTU’s test site for large wind turbine at Høvsøre, Denmark. Calibration is here understood as the establishment of a relation between the reference wind speed measurements with measurement...... uncertainties provided by measurement standard and corresponding lidar wind speed indications with associated measurement uncertainties. The lidar calibration concerns the 10 minute mean wind speed measurements. The comparison of the lidar measurements of the wind direction with that from wind vanes...... measurements are given for information only....

  17. Input model of a VVER 440/213 fuel assembly for the CFD computational code FLUENT

    The preparation of the input data and computation network for FLUENT 6.1 CFD (computational fluid dynamics) calculations by using the GAMBIT preprocessor is described. The input data for the thermal hydraulic calculation and the general issue of network creation - nodalization by using GAMBIT are highlighted. Creation of the particular computation network for the given fuel assembly geometry is described in detail. Attention was paid to the approach to the complex parts of the assembly, the inlet section in particular. The flow simulation in the fuel channel was analyzed. Solutions with lower numbers of channels and various degree of complexity were developed. The effect of the various solutions on the accuracy and time of calculation was investigated. The results were used to create the computation network of the whole assembly. In view of the complexity and volume of the network, the issue was discussed of how to find a suitable approach enabling test analyses to be performed on available hardware using available software

  18. 25 CFR 700.213 - Methods of providing last resort replacement housing.

    2010-04-01

    ... necessary, rehabilitation of a replacement dwelling; (5) The purchase of land and/or a replacement dwelling...) Rehabilitation of, and/or additions to, an existing replacement dwelling; (2) A replacement housing payment...

  19. {2-[(1,3-Benzothiazol-2-ylmethoxy]-5-fluorophenyl}(4-chlorophenylmethanone

    K. N. Venugopala

    2013-07-01

    Full Text Available The asymmetric unit of the title compound, C21H13ClFNO2S, contains two independent molecules with similar conformations. In the molecules, the thiazole ring is essentially planar [maximum atomic deviations = 0.014 (4 and 0.023 (5 Å] and is oriented with respect to the fluorophenyl ring and chlorophenyl rings at 9.96 (18 and 70.39 (18° in one molecule and at 7.50 (18 and 68.43 (18° in the other; the dihedral angles between the fluorophenyl and chlorophenyl rings are 64.9 (2 and 64.6 (2°, respectively. Intermolecular C—H...O and C—H...F hydrogen bonds stabilize the three-dimensional supramolecular architecture. Weak C—H...π and π–π interactions [centroid–centroid distance = 3.877 (3 Å] lead to a criss-cross molecular packing along the c axis.

  20. SU-D-213-06: Dosimetry of Modulated Electron Radiation Therapy Using Fricke Gel Dosimeter

    Purpose: Modulated electron radiation therapy (MERT) has been proposed as an effective modality for treatment of superficial targets. MERT utilizes multiple beams of different energies which are intensity modulated to deliver optimized dose distribution. Energy independent dosimeters are thus needed for quantitative evaluations of MERT dose distributions and measurements of absolute doses delivered to patients. Thus in the current work we study the feasibility of Fricke gel dosimeters in MERT dosimetry. Methods: Batches of radiation sensitive Fricke gel is fabricated and poured into polymethyl methacrylate cuvettes. The samples were irradiated in solid water phantom and a thick layer of bolus was used as a buildup. A spectrophotometer system was used for measuring the color changes (the absorbance) before and after irradiation and then we calculate net absorbance. We constructed calibration curves to relate the measured absorbance in terms of absorbed dose for all available electron energies. Dosimetric measurements were performed for mixed electron beam delivery and we also performed measurement for segmented field delivery with the dosimeter placed at the junction of two adjacent electron beams of different energies. Dose measured by our gel dosimetry is compared to that calculation from our precise treatment planning system. We also initiated a Monte Carlo study to evaluate the water equivalence of our dosimeters. MCBEAM and MCSIM codes were used for treatment head simulation and phantom dose calculation. PDDs and profiles were calculated for electron beams incident on a phantom designed with 1cm slab of Fricke gel. Results: The calibration curves showed no observed energy dependence with all studied electron beam energies. Good agreement was obtained between dose calculated and that obtained by gel dosimetry. Monte Carlo results illustrated the tissue equivalency of our Gel dosimeters. Conclusion: Fricke Gel dosimeters represent a good option for the dosimetric quality assurance prior to MERT application

  1. SU-C-213-06: Dosimetric Verification of 3D Printed Electron Bolus

    Rasmussen, K; Corbett, M; Pelletier, C; Huang, Z; Feng, Y; Jung, J [East Carolina Univ, Greenville, NC (United States)

    2015-06-15

    Purpose: To determine the dosimetric effect of 3D printed bolus in an anthropomorphic phantom. Methods: Conformable bolus material was generated for an anthropomorphic phantom from a DICOM volume. The bolus generated was a uniform expansion of 5mm applied to the nose region of the phantom, as this is a difficult area to uniformly apply bolus clinically. A Printrbot metal 3D Printer using PLA plastic generated the bolus. A 9MeV anterior beam with a 5cm cone was used to deliver dose to the nose of the phantom. TLD measurements were compared to predicted values at the phantom surface. Film planes were analyzed for the printed bolus, a standard 5mm bolus sheet placed on the phantom, and the phantom with no bolus applied to determine depth and dose distributions. Results: TLDs measured within 2.5% of predicted value for the 3D bolus. Film demonstrated a more uniform dose distribution in the nostril region for the 3d printed bolus than the standard bolus. This difference is caused by the air gap created around the nostrils by the standard bolus, creating a secondary build-up region. Both demonstrated a 50% central axis dose shift of 5mm relative to the no bolus film. HU for the bolus calculated the PLA electron density to be ∼1.1g/cc. Physical density was measured to be 1.3g/cc overall. Conclusion: 3D printed PLA bolus demonstrates improved dosimetric performance to standard bolus for electron beams with complex phantom geometry.

  2. Yeast Interacting Proteins Database: YGL213C, YBR059C [Yeast Interacting Proteins Database

    Full Text Available ription Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin...Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; invol...ved in endocytosis and actin cytoskeleton organization Rows with this prey as prey (3) Rows with this prey a

  3. (2R-2-(1,3-Dioxoisoindolin-2-yl-3-methylbutanoic acid

    Abdul Rauf Raza

    2011-09-01

    Full Text Available In the title compound, C13H13NO4, the dihedral angle between the nine-membered phthalimino ring system and the carboxylic acid group is 67.15 (9°. An intramolecular C—H...O close contact, which forms an S(6 ring, may help to establish the molecular conformation. In the crystal, molecules are linked by O—H...O hydrogen bonds, thereby forming C(7 chains propagating in [010].

  4. Study of aerosol particles produced by 213 nm laser ablation of different solid samples

    Holá, M.; Možná, V.; Kanický, V.; Mikuška, Pavel

    - : -, 2008 - (-). s. 199-200 ISBN N. [2008 Winter Conference on Plasma Spectrochemistry . 07.01.2008-12.01.2008, Temecula, CA] Institutional research plan: CEZ:AV0Z40310501 Keywords : aerosol particles * laser ablation Subject RIV: CB - Analytical Chemistry, Separation

  5. SU-B-213-06: Development of ABR Examination Questions

    The North American medical physics community validates the education received by medical physicists and the clinical qualifications for medical physicists through accreditation of educational programs and certification of medical physicists. Medical physics educational programs (graduate education and residency education) are accredited by the Commission on Accreditation of Medical Physics Education Programs (CAMPEP), whereas medical physicists are certified by several organizations, the most familiar of which is the American Board of Radiology (ABR). In order for an educational program to become accredited or a medical physicist to become certified, the applicant must meet certain specified standards set by the appropriate organization. In this Symposium, representatives from both CAMPEP and the ABR will describe the process by which standards are established as well as the process by which qualifications of candidates for accreditation or certification are shown to be compliant with these standards. The Symposium will conclude with a panel discussion. Learning Objectives: Recognize the difference between accreditation of an educational program and certification of an individual Identify the two organizations primarily responsible for these tasks Describe the development of educational standards Describe the process by which examination questions are developed GS is Executive Secretary of CAMPEP

  6. Pet image reconstruction with on-the-Fly Monte Carlo using GPU - 213

    Particle transport Monte Carlo is an inherently parallel algorithm and with all the further similarities to visible light ray-tracing it can easily be realized on current Graphics Processing Units (GPUs). This paper describes a GPU-based gamma photon transport code with application to the iterative image reconstruction steps of Positron Emission Tomography. The aim of the investigations here is the development of a Monte Carlo code capable of calculating the forward projection of each iteration step in the reconstruction with calculation times on the minute scale. Achieved simulation speed is in the order of 108 positrons per second. (authors)

  7. 49 CFR 212.213 - Motive power and equipment (MP&E) inspector.

    2010-10-01

    ... of determining compliance with all sections of the Freight Car Safety Standards (49 CFR part 215), Safety Glazing Standards (49 CFR part 223), Locomotive Safety Standards (49 CFR part 229), Safety Appliance Standards (49 CFR part 231), and Power Brake Standards (49 CFR part 232), to make reports of...

  8. 77 FR 213 - United States Savings Bonds, Series EE and I

    2012-01-04

    ... savings bonds, effective January 1, 2012. (See 76 FR 66,855 (Oct. 28, 2011)). In order to allow investors... Parts 351, 359, and 363 United States Savings Bonds, Series EE and I AGENCY: Bureau of the Public Debt... Series EE and Series I savings bonds a person may acquire each year. DATES: Effective January 4,...

  9. Crystal structure of 2-(1,3-dioxoindan-2-ylisoquinoline-1,3,4-trione

    Raza Murad Ghalib

    2015-01-01

    Full Text Available In the title isoquinoline-1,3,4-trione derivative, C18H9NO5, the five-membered ring of the indane fragment adopts an envelope conformation with the nitrogen-substituted C atom being the flap. The planes of the indane benzene ring and the isoquinoline-1,3,4-trione ring make a dihedral angle of 82.06 (6°. In the crystal, molecules are linked into chains extending along the bc plane via C—H...O hydrogen-bonding interactions, enclosing R22(8 and R22(10 loops. The chains are further connected by π–π stacking interations, with centroid-to-centroid distances of 3.9050 (7 Å, forming layers parallel to the b axis.

  10. CALiPER Report 21.3: Cost-Effectiveness of Linear (T8) LED Lamps

    Miller, Naomi J.; Perrin, Tess E.; Royer, Michael P.

    2014-05-27

    Meeting performance expectations is important for driving adoption of linear LED lamps, but cost-effectiveness may be an overriding factor in many cases. Linear LED lamps cost more initially than fluorescent lamps, but energy and maintenance savings may mean that the life-cycle cost is lower. This report details a series of life-cycle cost simulations that compared a two-lamp troffer using LED lamps (38 W total power draw) or fluorescent lamps (51 W total power draw) over a 10-year study period. Variables included LED system cost ($40, $80, or $120), annual operating hours (2,000 hours or 4,000 hours), LED installation time (15 minutes or 30 minutes), and melded electricity rate ($0.06/kWh, $0.12/kWh, $0.18/kWh, or $0.24/kWh). A full factorial of simulations allows users to interpolate between these values to aid in making rough estimates of economic feasibility for their own projects. In general, while their initial cost premium remains high, linear LED lamps are more likely to be cost-effective when electric utility rates are higher than average and hours of operation are long, and if their installation time is shorter.

  11. CALiPER Report 21.3. Cost Effectiveness of Linear (T8) LED Lamps

    None

    2014-05-01

    Meeting performance expectations is important for driving adoption of linear LED lamps, but cost-effectiveness may be an overriding factor in many cases. Linear LED lamps cost more initially than fluorescent lamps, but energy and maintenance savings may mean that the life-cycle cost is lower. This report details a series of life-cycle cost simulations that compared a two-lamp troffer using LED lamps (38 W total power draw) or fluorescent lamps (51 W total power draw) over a 10-year study period. Variables included LED system cost ($40, $80, or $120), annual operating hours (2,000 hours or 4,000 hours), LED installation time (15 minutes or 30 minutes), and melded electricity rate ($0.06/kWh, $0.12/kWh, $0.18/kWh, or $0.24/kWh). A full factorial of simulations allows users to interpolate between these values to aid in making rough estimates of economic feasibility for their own projects. In general, while their initial cost premium remains high, linear LED lamps are more likely to be cost-effective when electric utility rates are higher than average and hours of operation are long, and if their installation time is shorter.

  12. Earthquake design of switchgear cabinets of the VVER-440/213 at Paks

    The electrical and instrumentation and control area of NPP Paks is upgraded with new switchgear cabinets. In connection with the emergency power supply a rectifier switchgear cabinet is installed. The electric components inside, especially the extensive control electronics, are designed according to the German safety standard KTA 3703 (1986) as well as to withstand a high level earthquake. Investigated switchgear cabinets constructed as steel frames have plan dimensions of 800 x 1600 mm and heights of 2200 mm as well as total masses of approximately 1400 kgs. For the required earthquake design of the switchgear cabinets with safety relevant components inside the present seismic design basis of Parks is used. Proof of global stability of the steel frame as well as the determination of earthquake loading for the electric components mounted to the steel frame are established by Finite-Element-Calculations. The functionability of electric components during and after an earthquake is tested with real components by experimental earthquake simulation. For similar cabinets and equal or similar electric components the functionability is proved by analogy. This contribution presents the course of calculation and performance of tests as well as selected results. (author). 3 refs., 9 figs

  13. 24 CFR 213.277 - Right and liability under the Cooperative Management Housing Insurance Fund.

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Right and liability under the...-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES COOPERATIVE HOUSING MORTGAGE INSURANCE...

  14. 49 CFR 213.119 - Continuous welded rail (CWR); plan contents.

    2010-10-01

    ... identified in the following table: Minimum Number of Inspections per Calendar Year 1 Freight trains operating... provide an adequate history of track constructed with CWR. At a minimum, these records must include: (1... joints, including, at a minimum, periodic on-foot inspections; (2) Identify joint bars with visible...

  15. Level 2 PSA for the WWER 440/213 Dukovany NPP

    A brief review of PSA for the Dukovany NPP unit 1 is given. Main characteristics of the used methodology for PSA level 2, containment failure modes, PSA 1 and PSA 2 interface are discussed. Large event tree (APET) method, Hydrogen model, results for release classes and containment failure (case with PTS and without PTS) and these for plant damage state vectors are presented. Based on the obtained from PSA level 2 results the following conclusions have been made: 1) The limited scope PSA 2 proved to be a very good tool especially when comparing risk importance of individual phenomena, presently critical; 2) Before the next revision of limited scope PSA 2 for power states, some problems have to be solved, for example: better containment strength curve; better scenarios - MELCOR 1.8.5 analyses including SAMG; decreasing conservatism of natural leak from the intact containment; improved knowledge of steam explosions including cavity strength; 3) Next PSA 2 revision can not before 2005

  16. Analysis of a buried pipeline at WWER-440/213 Paks NPP

    According to regulations, all safety structures of a NPP must be designed to withstand loads induced by earthquakes. This applies to safety related underground piping systems. These structures are typically embedded in about 2-3 m of layered soil, and sometimes protected by concrete slabs resting on the ground surface. A rigorous solution for the dynamic response of such a structure would require accounting for nonlinear and three dimensional effects. A non-linear analysis is possible by using specialized computer codes and material models to account for non-linear behaviour of the soil. The aim of this paper was to reanalyze the pipeline of Paks NPP in order to demonstrate its dynamic behaviour interacting with the soil and the connected buildings as well as to determine the dynamic responses and the stresses in typical regions of the pipeline. To perform the numerical analysis a three dimensional finite element code (SSASSI/S) based on 'flexible volume method' was applied

  17. Pre-perihelion observations of Comet Halley at 2-13 microns

    Lynch, David K.; Russell, Ray W.; Retig, David A.; Rice, Carl J.; Young, Robert M.

    1986-01-01

    Between Nov. 8, 1985 and Jan. 13, 1986 comet Halley brightness was obtained as a function of time at 2.3, 3.6, 4.6, and 10.3 microns. Brightness outbursts were noted, an especially significant one beginning Jan. 10, 1986 and persisting for several days during which the IR brightness more than doubled. The heliocentric brightness indices for 2.3, 3.6, 4.6, and 10.3 microns are -5.7, -7.1, -8.5, and -6.2, respectively. Comet Halley's absolute magnitudes at these wavelengths are 7.3, 4.4, 2.8, and -1.8. The 4.6 to 10.3 micron color temperature dependence on heliocentric distance (R) is slightly less steep than the Rsup -1/2 expected theoretically. The albedo of the comet is 8% to 9%. Based on spectral differences between outbursts on Nov. 8, 1985 and Jan. 10 to 13, 1986, it is concluded that the comet displays composition inhomogeneities.

  18. Characterization of neutron detector combined with NE213 and CaF{sub 2}(Eu)

    Takada, Masashi; Nakamura, Takashi [Tohoku Univ., Sendai (Japan). Cyclotron and Radioisotope Center; Sibata, Tokushi

    1996-07-01

    In this work, the property of the n-{gamma} discrimination and the response functions of the developed phoswich detector were measured with gamma rays from radioactive sources and neutrons from a Be+Cu target bombarded by protons from the RIKEN ring cyclotron at the Institute of Physical and Chemical Research. The measured response functions were compared with Monte Carlo calculations. We also tested to measure a small amount of neutrons under the intense charged-particles mixed field which was realized in the RIKEN ring cyclotron for the space application. (J.P.N.)

  19. 8 CFR 213a.4 - Actions for reimbursement, public notice, and congressional reports.

    2010-01-01

    ..., or joint sponsor, by personal service, as defined by 8 CFR 103.5a(a)(2), except that the person... obtain the sponsored immigrant's current immigration or citizenship status or the name, social security... immigrant wants to seek reimbursement from a sponsor, household member, or joint sponsor, the...

  20. SU-C-213-03: Custom 3D Printed Boluses for Radiation Therapy

    Zhao, B; Yang, M; Yan, Y; Rahimi, A; Chopra, R; Jiang, S [UT Southwestern Medical Center, Dallas, TX (United States)

    2015-06-15

    Purpose: To develop a clinical workflow and to commission the process of creating custom 3d printed boluses for radiation therapy. Methods: We designed a workflow to create custom boluses using a commercial 3D printer. Contours of several patients were deformably mapped to phantoms where the test bolus contours were designed. Treatment plans were created on the phantoms following our institutional planning guideline. The DICOM file of the bolus contours were then converted to stereoLithography (stl) file for the 3d printer. The boluses were printed on a commercial 3D printer using polylactic acid (PLA) material. Custom printing parameters were optimized in order to meet the requirement of bolus composition. The workflow was tested on multiple anatomical sites such as skull, nose and chest wall. The size of boluses varies from 6×9cm2 to 12×25cm2. To commission the process, basic CT and dose properties of the printing materials were measured in photon and electron beams and compared against water and soft superflab bolus. Phantoms were then scanned to confirm the placement of custom boluses. Finally dose distributions with rescanned CTs were compared with those computer-generated boluses. Results: The relative electron density(1.08±0.006) of the printed boluses resemble those of liquid tap water(1.04±0.004). The dosimetric properties resemble those of liquid tap water(1.04±0.004). The dosimetric properties were measured at dmax with an ion chamber in electron and photon open beams. Compared with solid water and soft bolus, the output difference was within 1% for the 3D printer material. The printed boluses fit well to the phantom surfaces on CT scans. The dose distribution and DVH based on the printed boluses match well with those based on TPS generated boluses. Conclusion: 3d printing provides a cost effective and convenient solution for patient-specific boluses in radiation therapy.

  1. People and things. CERN Courier, April 1981, v. 21(3)

    The article reports on achievements of various people, staff changes and position opportunities within the CERN organization and contains news updates on upcoming or past events. The detail of the LEP electron-positron storage ring project continues to be studied so as to optimize the machine parameters from the point of view of performance and of cost. The Weizmann Institute of Science in Israel has established the Albert Einstein Centre for Theoretical Physics with an endowment fund from the Federal Republic of Germany. Aimed at strengthening the ties and exchange of ideas between theoretical physicists from Israel and abroad the Centre plans to host postdoctoral fellows and visiting senior theorists. After several years of planning, a major upgrade has now been decided for the central computer system at SLAC, presently based on IBM 360/91 and 370/168 machines. Fermilab has established an Industrial Affiliate Programme designed to foster improved communication between the basic research carried out at Fermilab and in industry. The affiliate programme will acquaint the Industrial Associates with work at Fermilab through special seminars and publications. A further indication of the commitment of Stanford to the linear collider project (SLC) is the organization of a meeting on 25-27 March to inform the US high energy physics community of the progress of the project, to promote involvement in machine design and to indicate the experimental possibilities it will provide. Although now firmly established as part of particle physics dogma, the quark model has had a somewhat chequered history. Before the discovery of the omega minus baryon in 1964, it had a particularly rough time. In an invited talk at last year's Baryon Conference in Toronto, George Zweig of Caltech reminisced about the early years of the model

  2. SU-C-213-06: Dosimetric Verification of 3D Printed Electron Bolus

    Purpose: To determine the dosimetric effect of 3D printed bolus in an anthropomorphic phantom. Methods: Conformable bolus material was generated for an anthropomorphic phantom from a DICOM volume. The bolus generated was a uniform expansion of 5mm applied to the nose region of the phantom, as this is a difficult area to uniformly apply bolus clinically. A Printrbot metal 3D Printer using PLA plastic generated the bolus. A 9MeV anterior beam with a 5cm cone was used to deliver dose to the nose of the phantom. TLD measurements were compared to predicted values at the phantom surface. Film planes were analyzed for the printed bolus, a standard 5mm bolus sheet placed on the phantom, and the phantom with no bolus applied to determine depth and dose distributions. Results: TLDs measured within 2.5% of predicted value for the 3D bolus. Film demonstrated a more uniform dose distribution in the nostril region for the 3d printed bolus than the standard bolus. This difference is caused by the air gap created around the nostrils by the standard bolus, creating a secondary build-up region. Both demonstrated a 50% central axis dose shift of 5mm relative to the no bolus film. HU for the bolus calculated the PLA electron density to be ∼1.1g/cc. Physical density was measured to be 1.3g/cc overall. Conclusion: 3D printed PLA bolus demonstrates improved dosimetric performance to standard bolus for electron beams with complex phantom geometry

  3. 30 CFR 250.213 - What general information must accompany the EP?

    2010-07-01

    ... OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Plans and Information Contents... 40 CFR part 302, or amounts specified by the Regional Supervisor. (d) New or unusual technology. A... general subject matter of the omitted information. If you will not use any new or unusual technology...

  4. The evaluation of the bubble condenser containment of VVER-440/213 plants

    A number of pressurized water reactors of the VVER-440 type have a containment building which is connected to a bubble condenser unit reducing the design pressure of the entire containment system. The bubble condenser acts as a pressure suppression system by condensation of released steam. Some characteristic features of this system are presented. Refering to experience with the assessement of Western pressure suppression system containments of boiling water reactors (BWRs) the experimental and analytical background of the bubble condenser design has been studied. Several earlier test rigs located in the territory of the former Soviet Union and in other Eastern European countries have been studied and assessed. These test rigs served to prove the thermal efficiency of this arrangement. Characteristic properties of pressures suppression systems of Western BWRs are compared with the properties of bubble condensers and some existing test rigs. An international working group has identified a number of open questions for which neither experimental evidence nor sound analytical predictions exist. Of main interest are the response of bubble condenser structures to oscillatory condensation processes, the simultaneous interaction of a large number of gap-cap units and additionally some unresolved scale-up problems. Based on these findings a supplementary experimental research program has been proposed to answer these open questions. The suitability of existing test rigs and of a new experimental model have been assessed. The results are summarized. In view of the importance of the containment effectiveness for public safety the proposed research work deserves a high priority within a program to assist countries utilizing VVER-440 reactors in confirming an adequate level of reactor safety. (orig.)

  5. SU-D-213-06: Dosimetry of Modulated Electron Radiation Therapy Using Fricke Gel Dosimeter

    Gawad, M Abdel; Elgohary, M; Hassaan, M; Emam, M [Al Azhar University, Cairo Egypt (Egypt); Desouky, O [National center for radiation research and technology-Egyptian atomic energy, Cairo (Egypt); Eldib, A [Al Azhar University, Cairo Egypt (Egypt); Fox Chase Cancer Center, Philadelphia, PA (United States); Ma, C [Fox Chase Cancer Center, Philadelphia, PA (United States)

    2015-06-15

    Purpose: Modulated electron radiation therapy (MERT) has been proposed as an effective modality for treatment of superficial targets. MERT utilizes multiple beams of different energies which are intensity modulated to deliver optimized dose distribution. Energy independent dosimeters are thus needed for quantitative evaluations of MERT dose distributions and measurements of absolute doses delivered to patients. Thus in the current work we study the feasibility of Fricke gel dosimeters in MERT dosimetry. Methods: Batches of radiation sensitive Fricke gel is fabricated and poured into polymethyl methacrylate cuvettes. The samples were irradiated in solid water phantom and a thick layer of bolus was used as a buildup. A spectrophotometer system was used for measuring the color changes (the absorbance) before and after irradiation and then we calculate net absorbance. We constructed calibration curves to relate the measured absorbance in terms of absorbed dose for all available electron energies. Dosimetric measurements were performed for mixed electron beam delivery and we also performed measurement for segmented field delivery with the dosimeter placed at the junction of two adjacent electron beams of different energies. Dose measured by our gel dosimetry is compared to that calculation from our precise treatment planning system. We also initiated a Monte Carlo study to evaluate the water equivalence of our dosimeters. MCBEAM and MCSIM codes were used for treatment head simulation and phantom dose calculation. PDDs and profiles were calculated for electron beams incident on a phantom designed with 1cm slab of Fricke gel. Results: The calibration curves showed no observed energy dependence with all studied electron beam energies. Good agreement was obtained between dose calculated and that obtained by gel dosimetry. Monte Carlo results illustrated the tissue equivalency of our Gel dosimeters. Conclusion: Fricke Gel dosimeters represent a good option for the dosimetric quality assurance prior to MERT application.

  6. 5 CFR 532.213 - Industries included in regular appropriated fund wage surveys.

    2010-01-01

    ... transportation except taxi and limousine service (NAICS 4853). 487 (except 4872) Scenic and sightseeing transportation except scenic and sightseeing transportation, water (NAICS 4872). 488 (except 4883 and 4884... support activities for road transportation (NAICS 4884). 492 Couriers and messengers. 493 Warehousing...

  7. Radioactive sludge and wastewater analysis and treatment in the Hungarian VVER-440/213-type NPP

    It is well known that in the Hungarian VVER-type nuclear power plant Paks the radioactive waste waters are collected in common tanks. These water streams contain radioactive isotopes in ultra-low concentration and inactive compounds as major components (borate 1.7 g/dm3, sodium-nitrate 0.4 g/dm3, sodium-hydroxide 0.16 g/dm3, and oxalate 0.25 g/dm3). These low salinity solutions were evaporated by adding sodium-hydroxide, until 400 g/dm3 salt content is reached. There is about 6000 m3 concentrated evaporator bottom residues in the tanks of the reactor. There are some tanks at the power plant containing sludge type radioactive waste containing more or less liquid phase too. The general physical and chemical characteristics (density, pH, total solid, dissolved solid etc.) and chemical and radiochemical composition are important information for volume reduction and solidification treatment of these wastes. We have investigated and constructed a complex analysis system for the radioactive sludge and supernatant analysis, including the physical, as well as the chemical and radiochemical analysis methods. Using well known analysis techniques as ion chromatography, ICP-MS, AAS, gamma-and alpha-spectrometry and chemical alkaline fusion digestion and acidic dissolution methods we could analyze the main inorganic, organic and radioactive components of the sludges and supernatants. Determination of the mass and charge balance for the sludge samples were more difficult then for the supernatant samples. Not only are there assumptions required about the chemical form and the oxidation state of the species present in the sludge, but many of the compounds in the sludge are mixed oxides which are not directly measured. Also, the sludge is actually a slurry with a high water content. The interstitial liquid is in close contact with the sludge, and there are many ionic solubility equilibriums. The anion data for the sludge samples are based on the water soluble anions that would be available to a water wash. The water wash would not account for the insoluble hydroxides, carbonates, and mixed oxides present. The insoluble species do not contribute to the charge balance, and the cation charge is not used in the calculation. Most of the nitrate reported for the sludge is due to the interstitial liquid. Considering the limitations of these calculations, the mass balance was within the analytical error (±20%) for the sludge samples. There were three sample preparation methods used to investigate the total anion content of the sludge samples, which included water leach, potassium-hydroxide and/or sodium peroxide/sodium hydroxide fusion and acidic dissolution. (author)

  8. An Experimental Study of Thermal Fatigue on ASTM A 213 Grade T-23 Steel Tube

    Jinu, G. R.; P.Sathiya; Ravichandran, G; Rathinam, A.

    2009-01-01

    Super heater tubes are subjected to alternate heating and cooling in power plants causes crack and eventually fail. This phenomenon is termed as thermal fatigue. In this paper, a laboratory simulation for reproducing thermal fatigue phenomenon is developed to determine the number of cycles of failure occurs in super heater tubes. Thermal fatigue tests are conducted in Non-Destructive Tested T23 base and SMAW welded tubes separately. The tubes are subjected to thermal cycles from 800∘C (accele...

  9. Comparison of thermal fatigue behaviour of ASTM A 213 grade T-92 base and weld tubes

    Super-heater tubes are subjected to alternate heating and cooling in power plants causing them to crack and eventually fail. This phenomenon is referred to as 'thermal fatigue.' In this paper, a laboratory simulation for reproducing the thermal fatigue phenomenon is developed to determine the number of cycles necessary before failure occurs in super-heater tubes. The temperature and strain distributions along the specimen were computed theoretically using ANSYS software for the applied temperature condition. The thermal fatigue test was conducted for both base and shielded metal arc (SMA) welded tubes separately and both passed in the non-destructive tests. These tubes were subjected to thermal cycles from 800 .deg. C (accelerated temperature) to room temperature. Oxy-acetylene heating setup was utilized as a heating source, and a water bath was utilized for quenching purposes. The tests were carried out until open cracks were identified. Surface cracks were identified in the base and weld tubes after 90 and 60 cycles respectively. This study reveals that heating and cooling cause thermal fatigue, initiate cracks in the tubes

  10. SU-B-213-05: Development of ABR Certification Standards

    The North American medical physics community validates the education received by medical physicists and the clinical qualifications for medical physicists through accreditation of educational programs and certification of medical physicists. Medical physics educational programs (graduate education and residency education) are accredited by the Commission on Accreditation of Medical Physics Education Programs (CAMPEP), whereas medical physicists are certified by several organizations, the most familiar of which is the American Board of Radiology (ABR). In order for an educational program to become accredited or a medical physicist to become certified, the applicant must meet certain specified standards set by the appropriate organization. In this Symposium, representatives from both CAMPEP and the ABR will describe the process by which standards are established as well as the process by which qualifications of candidates for accreditation or certification are shown to be compliant with these standards. The Symposium will conclude with a panel discussion. Learning Objectives: Recognize the difference between accreditation of an educational program and certification of an individual Identify the two organizations primarily responsible for these tasks Describe the development of educational standards Describe the process by which examination questions are developed GS is Executive Secretary of CAMPEP

  11. SU-C-213-03: Custom 3D Printed Boluses for Radiation Therapy

    Purpose: To develop a clinical workflow and to commission the process of creating custom 3d printed boluses for radiation therapy. Methods: We designed a workflow to create custom boluses using a commercial 3D printer. Contours of several patients were deformably mapped to phantoms where the test bolus contours were designed. Treatment plans were created on the phantoms following our institutional planning guideline. The DICOM file of the bolus contours were then converted to stereoLithography (stl) file for the 3d printer. The boluses were printed on a commercial 3D printer using polylactic acid (PLA) material. Custom printing parameters were optimized in order to meet the requirement of bolus composition. The workflow was tested on multiple anatomical sites such as skull, nose and chest wall. The size of boluses varies from 6×9cm2 to 12×25cm2. To commission the process, basic CT and dose properties of the printing materials were measured in photon and electron beams and compared against water and soft superflab bolus. Phantoms were then scanned to confirm the placement of custom boluses. Finally dose distributions with rescanned CTs were compared with those computer-generated boluses. Results: The relative electron density(1.08±0.006) of the printed boluses resemble those of liquid tap water(1.04±0.004). The dosimetric properties resemble those of liquid tap water(1.04±0.004). The dosimetric properties were measured at dmax with an ion chamber in electron and photon open beams. Compared with solid water and soft bolus, the output difference was within 1% for the 3D printer material. The printed boluses fit well to the phantom surfaces on CT scans. The dose distribution and DVH based on the printed boluses match well with those based on TPS generated boluses. Conclusion: 3d printing provides a cost effective and convenient solution for patient-specific boluses in radiation therapy

  12. Aviation Structural Mechanic, Second Class, 2-13. Military Curriculum Materials for Vocational and Technical Education.

    Ohio State Univ., Columbus. National Center for Research in Vocational Education.

    This self-paced, individualized course, adapted from military curriculum materials for use in vocational and technical education, teaches students the skills needed to become aviation structural mechanics (second class). The course materials consist of five pamphlets covering the structural maintenance and repair of aircraft. The first pamphlet…

  13. 2-[1-(3-Amino­phenyl­imino)­eth­yl]phenol

    Blagus, Anita; Kaitner, Branko

    2011-01-01

    The title compound, C14H14N2O, exists as the enol–imine tautomer. A strong intra­molecular hydrogen bond between O and N atoms forms a six-membered ring with an S(6) graph-set motif, which is approximately coplanar with the phenol ring, the inter­planar angle being 3.4 (3)°. In the crystal, inter­molecular C—H⋯O hydrogen bonds and N—H⋯π inter­actions link the mol­ecules into infinite chains along [100].

  14. Generator method of 225Ac production without a carrier for nuclear medicine

    The two-steps isotope generator scheme of 225Ac production from 229Th has been developed. The first step is used for separation of thorium, actinium, radium and daughter decay products (DDP), and removals of parent radionuclide. The second step provides additional separation of actinium from traces of radium and DDP, and conversion of actinium in the nitrate form. The chosen solutions provide optimal conditions for carry out of process. The yield of the 225Ac was 99.9% at minimal losses of parent 229Th (less than 0.1%)

  15. Orthorectification and Mosaicking of Color Aerial Photography for the Main Eight Hawaiian Islands: Oahu (213-214e-0516)

    National Oceanic and Atmospheric Administration, Department of Commerce — Habitat maps of the main Hawaiian Islands were created by visual interpretation of aerial photos and hyperspectral imagery using the Habitat Digitizer extension....

  16. Integrated safety assessment report, Haddam Neck Plant (Docket No. 50-213): Integrated Safety Assessment Program: Draft report

    The operating history of the plant from 1967 through 1984 was reviewed and analyzed. The findings of the review identified trends and symptoms in the operating data that can be used as tools in the resolution and prioritization of the Haddam Neck safety issues. The review included evaluation of data collected on plant availability and capacity factors, forced shutdowns, power reductions, reportable events, and environmental considerations. The methodology used is also discussed. Data and information is presented in appendices. Haddam Neck Plant has generally been operated in a safe and orderly manner, with a trend that shows continuous improvement. Perturbations to this trend were correlated to an extensive turbine refit, post-effects of an extended operating run, and effects of efforts applied in response to TMI. Three specific symptoms of continuing safety related problems were recurrence of loss of offsite power events, control rod drive failures, and charging pump failures. 24 refs., 21 figs., 11 tabs

  17. Assessing influence of the auxiliary emergency feedwater system on Rivne-1,2 WWER-440/213 core damage frequency

    The influence of the auxiliary emergency feedwater system on Rivne-1 ana 2 core damage frequency is assessed in this paper. The influence is assessed by means of probabilistic safety analysis methods. Results of preliminary and already implemented designs of the system are compared. Besides, the paper presented specifics of modeling the system elements

  18. PS2-13: Genetic and Environmental Risk Factors for Prescription Opioid Dependence in the Healthcare Setting

    Boscarino, Joseph A.; Hoffman, Stuart N.; Gerhard, Glenn; Han, John; Rukstalis, Margaret; Erlich, Porat M.; Stewart, Walter F.

    2010-01-01

    Background: Research on the genetics of opioid addiction among illicit drug users is difficult given challenges to case ascertainment and selection of an appropriate control group. To address this limitation, we tested a model by studying prescription opioid users.

  19. [Nebraska 4-H Wheat Science School Enrichment Project, Teacher/Leader Guides 213-222 and 227.

    Nebraska Univ., Lincoln. Inst. of Agriculture and Natural Resources.

    Through the 4-H Wheat Science project, students learn the importance of wheat from the complete process of growing wheat to the final product of bread. The curriculum is designed to include hands-on experiences in science, consumer education, nutrition, production economics, vocabulary, and applied mathematics. Teachers can select those units out…

  20. SU-D-213-02: Characterization of the Effect of a New Commercial Transmission Detector On Radiotherapy Beams

    Purpose: To evaluate the influence of a new commercial transmission detector on radiotherapy beams of various energies. Methods: A transmission detector designed for online treatment monitoring was characterized on a TrueBeam STx linear accelerator with 6MV, 6FFF, 10MV, and 10FFF beams. Measurements of beam characteristics including percentage depth doses (PDDs), inplane and crossplane off-axis profiles at different depths, transmission factors, and skin dose were acquired at field sizes of 3×3cm, 5×5m, 10×10cm, and 20×20cm at 100cm and 80cm source-to-surface distance (SSD). All measurements were taken with and without the transmission detector in the path of the beam. A CC04 chamber was used for all profile and transmission factor measurements. Skin dose was assessed at 100cm, 90cm, and 80cm SSD and using a variety of detectors (Roos and Markus parallel-plate chambers, and OSLD). Results: The PDDs showed small differences between the unperturbed and perturbed beams for both 100cm and 80cm SSD (≤4mm dmax difference and <1.2% average profile difference). The differences were larger for the flattened beams and at larger field sizes. The off-axis profiles showed similar trends. The penumbras looked similar with and without the transmission detector. Comparisons in the central 80% of the profile showed a maximum average (maximum) profile difference between all field sizes of 0.756% (1.535%) and 0.739% (3.682%) for 100cm and 80cm SSD, respectively. The average measured skin dose at 100cm (80cm) SSD for 10×10cm field size was <4% (<35%) dose increase for all energies. For 20×20cm field size, this value increased to <10% (≤45%). Conclusion: The transmission detector has minimal effect on the clinically relevant radiotherapy beams for IMRT and VMAT (field sizes 10×10cm and less). For larger field sizes, some perturbations are observable which would need to be assessed for clinical impact. The authors of this publication has research support from IBA Dosimetry

  1. Integrated plant safety assessment: systematic evaluation program. Haddam Neck Plant, Connecticut Yankee Atomic Power Company. Docket No. 50-213

    The Systematic Evaluation Program was initiated in February 1977 by the US Nuclear Regulatory Commission to review the designs of older operating nuclear reactor plants to confirm and document their safety. The review provides: (1) an assessment of how these plants compare with current licensing safety requirements relating to selected issues, (2) a basis for deciding on how these differences should be resolved in an integrated plant review, and (3) a documented evaluation of plant safety. This report documents the review of Haddam Neck Plant, operated by Connecticut Yankee Atomic Power Company. The Haddam Neck Plant is one of 10 plants reviewed under Phase II of this program. This report indicates how 137 topics selected for review under Phase I of the program were addressed. Equipment and procedural changes have been identified as a result of the review

  2. SU-C-213-02: Characterizing 3D Printing in the Fabrication of Variable Density Phantoms

    Purpose: In this work, we present characterization, process flow, quality control and application of 3D fabricated low density phantoms for radiotherapy quality assurance. Methods: A Rostock delta 3D printer using polystyrene filament of diameter 1.75 mm was used to print geometric volumes of 2×2×1 cm3 of varying densities. The variable densities of 0.1 to 0.75 g/cm 3 were created by modulating the infill. A computed tomography (CT) scan was performed to establish an infill-density calibration curve as well as characterize the quality of the print such as uniformity and the infill pattern. The time required to print these volumes was also recorded. Using the calibration, two low density cones (0.19, 0.52 g/cm3) were printed and benchmarked against commercially available phantoms. The dosimetric validation of the low density scaling of Anisotropic Analytical Algorithm (AAA) was performed by using a 0.5 g/cm3 slab of 10×10×2.4 cm3 with EBT3 GafChromic film. The gamma analysis at 3%/3mm criteria were compared for the measured and computed dose planes. Results: Analysis of the volume of air pockets in the infill resulted in a reasonable uniformity for densities 0.4 to 0.75 g/cm3. Printed phantoms with densities below 0.4 g/cm3 exhibited a higher ratio of air to polystyrene resulting in large non-uniformity. Compared to the commercial inserts, good agreement was observed only for the printed 0.52 g/cm3 cone. Dosimetric comparison for a printed low density volume placed in-between layers of solid water resulted in >95% gamma agreement between AAA calculated dose planes and measured EBT3 films for a 6MV 5×5 cm2 clinical beam. The comparison showed disagreement in the penumbra region. Conclusion: In conclusion, 3D printing technology opens the door to desktop fabrication of variable density phantoms at economical prices in an efficient manner for the quality assurance needs of a small clinic

  3. Recent results of three-dimensional CFD simulations of coolant mixing in VVER-440/213 reactor pressure vessel

    The Budapest University of Technology and Economics, Institute of Nuclear Techniques has been working since 2001 on the three-dimensional CFD model of the reactor pressure vessel of the VVER-440 type reactor. During this time period - due to the development of the available computational capacity - a very complex and detailed model of the RPV has been developed. The aim of the construction of the new model is to describe further internal structures of the RPV (e.g. correct modeling of brake tubes, or internals in the upper mixing chamber) and to perform an extensive sensitivity analysis on the different modeling and calculation parameters (e.g. porous region models vs. detailed modeling, or n different turbulence models). The new model can be applied for steady state calculation during normal operational condition and for different transient analyses as well. One interesting application is the participation in a planned benchmark exercise on the start-up of the sixth main coolant pump, which is aimed to compare the capabilities of mixing models of one-dimensional system codes with the results of CFD simulation. (authors)

  4. SU-C-213-02: Characterizing 3D Printing in the Fabrication of Variable Density Phantoms

    Madamesila, J; McGeachy, P; Villarreal-Barajas, J; Khan, R [The University of Calgary, Calgary, AB (Canada)

    2015-06-15

    Purpose: In this work, we present characterization, process flow, quality control and application of 3D fabricated low density phantoms for radiotherapy quality assurance. Methods: A Rostock delta 3D printer using polystyrene filament of diameter 1.75 mm was used to print geometric volumes of 2×2×1 cm{sup 3} of varying densities. The variable densities of 0.1 to 0.75 g/cm {sup 3} were created by modulating the infill. A computed tomography (CT) scan was performed to establish an infill-density calibration curve as well as characterize the quality of the print such as uniformity and the infill pattern. The time required to print these volumes was also recorded. Using the calibration, two low density cones (0.19, 0.52 g/cm{sup 3}) were printed and benchmarked against commercially available phantoms. The dosimetric validation of the low density scaling of Anisotropic Analytical Algorithm (AAA) was performed by using a 0.5 g/cm{sup 3} slab of 10×10×2.4 cm{sup 3} with EBT3 GafChromic film. The gamma analysis at 3%/3mm criteria were compared for the measured and computed dose planes. Results: Analysis of the volume of air pockets in the infill resulted in a reasonable uniformity for densities 0.4 to 0.75 g/cm{sup 3}. Printed phantoms with densities below 0.4 g/cm{sup 3} exhibited a higher ratio of air to polystyrene resulting in large non-uniformity. Compared to the commercial inserts, good agreement was observed only for the printed 0.52 g/cm{sup 3} cone. Dosimetric comparison for a printed low density volume placed in-between layers of solid water resulted in >95% gamma agreement between AAA calculated dose planes and measured EBT3 films for a 6MV 5×5 cm{sup 2} clinical beam. The comparison showed disagreement in the penumbra region. Conclusion: In conclusion, 3D printing technology opens the door to desktop fabrication of variable density phantoms at economical prices in an efficient manner for the quality assurance needs of a small clinic.

  5. 2-(1,3-Dioxoisoindolin-2-ylacetic acid–N′-[(E-2-methoxybenzylidene]pyridine-4-carbohydrazide (1/1

    Shaaban K. Mohamed

    2012-08-01

    Full Text Available In the title 1:1 cocrystal, C10H7NO4·C14H13N3O2, molecules are linked by intermolecular C—H...O, N—H...O and O—H...N hydrogen bonds, forming a three-dimensional network. In addition, π–π stacking interactions [with centroid–centroid distances of 3.5723 (19 and 3.6158 (18 Å] are observed.

  6. Oahu Photomosaic 2000 (213-214e-0516) - Orthorectification and Mosaicing of Color Aerial Photography Main Eight Hawaiian Islands

    National Oceanic and Atmospheric Administration, Department of Commerce — Habitat maps of the main Hawaiian Islands were created by visual interpretation of aerial photos and hyperspectral imagery using the Habitat Digitizer extension....

  7. Oahu Photomosaic 2000 (213-214w-0516) - Orthorectification and Mosaicing of Color Aerial Photography Main Eight Hawaiian Islands

    National Oceanic and Atmospheric Administration, Department of Commerce — Habitat maps of the main Hawaiian Islands were created by visual interpretation of aerial photos and hyperspectral imagery using the Habitat Digitizer extension....

  8. Orthorectification and Mosaicking of Color Aerial Photography for the Main Eight Hawaiian Islands: Oahu (213-214w-0516)

    National Oceanic and Atmospheric Administration, Department of Commerce — Habitat maps of the main Hawaiian Islands were created by visual interpretation of aerial photos and hyperspectral imagery using the Habitat Digitizer extension....

  9. SU-D-213-07: Initial Characterization of a Gel Patch Dosimeter for in Vivo Dosimetry

    Purpose: In vivo dosimetry, despite being the most direct method for monitoring the dose delivered during radiation therapy and being recommended by several national and international organizations (AAPM, ICRU, NACP), is underutilized in the clinic due to issues associated with dose sensitivity, feasibility, and cost. Given the increasing complexity of radiation therapy modern treatments, there is a compelling need for a robust, affordable in vivo dosimetry option. In this work we present the initial characterization of a novel gel patch in vivo dosimeter. Methods: DEFGEL (6%T) was used to make 1-cm thick small cylindrical patch dosimeters. The optical density of each dosimeter was read before and after irradiation by an in-house laser densitometer. The dosimeters were irradiated using a Varian Clinac EX linac. Three separate batches of gel patches were used to create dose response curves and evaluate repeatability. The development time of the dosimeter was also evaluated. Results: The dose response of the dosimeter was found to be linear from a range of approximately 1-Gy to 20-Gy, which is a larger window of linearity compared to other in vivo dosimeters. At doses below 1-Gy, the cumulative uncertainties were on the order of the measured data. When compared, the three batches demonstrated repeatability from 1-Gy to approximately 13-Gy, with some variation at higher doses. For doses of >8-Gy, the dosimeter reached full optical density after 4-hours, whereas low doses developed within an hour. Conclusion: Initial results indicate that the gel patch dosimeter is a reliable and simple way to measure a large range of doses, including high doses such as those delivered during hypofractionated treatments (e.g. SBRT or MR-guided radiotherapy). The simple fabrication method for the dosimeter and the use of a laser densitometer would allow for the dosimeter to used and read in-house, cheaply and easily

  10. Integrated safety assessment report, Haddam Neck Plant (Docket No. 50-213): Integrated Safety Assessment Program: Draft report

    The integrated assessment is conducted on a plant-specific basis to evaluate all licensing actions, licensee initiated plant improvements and selected unresolved generic/safety issues to establish implementation schedules for each item. Procedures allow for a periodic updating of the schedules to account for licensing issues that arise in the future. The Haddam Neck Plant is one of two plants being reviewed under the pilot program. This report indicates how 82 topics selected for review were addressed, and presents the staff's recommendations regarding the corrective actions to resolve the 82 topics and other actions to enhance plant safety. 135 refs., 4 figs., 5 tabs

  11. High energy phenomena around collapsed stars; Proceedings of the NATO Advanced Study Institute, Cargese, France, Sept. 2-13, 1985

    Pacini, F.

    Recent studies of high-energy phenomena around collapsed stars are reported. The topics considered include: stellar evolution and the formation of binary systems, observations of supernovae, the dynamics of supernova envelopes, stellar collapse and supernova explosions, comparison of white dwarfs and neutron stars, observations of pulsars, energetic radiation from magnetized neutron stars, neutron stars as cosmic hadron physical laboratories, recent developments on the Crab Nebula, and the association between supernova remnants and pulsars. Also addressed are: progenitors and products of supernovae, plerions in theory and practice, binary origin of pulsar velocities, black holes and gamma-ray sources in the Galaxy, cosmic gamma ray bursts, propagation and origin of Galactic cosmic rays, information on the boundary layer of quasi-periodic oscillations in the Galactic bulge sources using power spectra, and the 35-day cycle of Her X-1.

  12. SU-D-213-07: Initial Characterization of a Gel Patch Dosimeter for in Vivo Dosimetry

    Matrosic, C; Culberson, W; Rosen, B; Madsen, E; Frank, G; Bednarz, B [Univ of Wisc Madison, Madison, WI (United States)

    2015-06-15

    Purpose: In vivo dosimetry, despite being the most direct method for monitoring the dose delivered during radiation therapy and being recommended by several national and international organizations (AAPM, ICRU, NACP), is underutilized in the clinic due to issues associated with dose sensitivity, feasibility, and cost. Given the increasing complexity of radiation therapy modern treatments, there is a compelling need for a robust, affordable in vivo dosimetry option. In this work we present the initial characterization of a novel gel patch in vivo dosimeter. Methods: DEFGEL (6%T) was used to make 1-cm thick small cylindrical patch dosimeters. The optical density of each dosimeter was read before and after irradiation by an in-house laser densitometer. The dosimeters were irradiated using a Varian Clinac EX linac. Three separate batches of gel patches were used to create dose response curves and evaluate repeatability. The development time of the dosimeter was also evaluated. Results: The dose response of the dosimeter was found to be linear from a range of approximately 1-Gy to 20-Gy, which is a larger window of linearity compared to other in vivo dosimeters. At doses below 1-Gy, the cumulative uncertainties were on the order of the measured data. When compared, the three batches demonstrated repeatability from 1-Gy to approximately 13-Gy, with some variation at higher doses. For doses of >8-Gy, the dosimeter reached full optical density after 4-hours, whereas low doses developed within an hour. Conclusion: Initial results indicate that the gel patch dosimeter is a reliable and simple way to measure a large range of doses, including high doses such as those delivered during hypofractionated treatments (e.g. SBRT or MR-guided radiotherapy). The simple fabrication method for the dosimeter and the use of a laser densitometer would allow for the dosimeter to used and read in-house, cheaply and easily.

  13. Severe accident management development program for VVER-1000 and VVER-440/213 based on the westinghouse owners group approach

    The development of the Westinghouse Owners Group Severe Accident Management Guidelines (WOG SAMG) between 1991 and 1994 was initiated in response to the U.S. Nuclear Regulatory Commission (NRC) requirement for addressing the regulatory severe accident concerns. Hence, the WOG SAMG is designed to interface with other existing procedures at the plant and is used in accident sequences that have progressed to the point where these other procedures are not applicable any longer, i.e. following core damage. The primary purpose of the WOG SAMG is to reach a controlled stable state, which can be declared when fission product releases are controlled, challenges to the confinement fission product boundary have been mitigated, and adequate heat removal is provided to the core and the containment. Although the WOG SAMG is a generic severe accident management guidance developed for use by the entirety of the operating Westinghouse PWR plants, provisions have been made in their development to address specific features of individual plants such as confinement type and the feasibility of reactor cavity flooding. Similarly, the generic SAMG does not address unique plant features and equipment, but rather allows for consideration of plant specific features and strategies. This adaptable approach has led to several SAMG development programs for VVER-1000 and VVER-440 type of power plants, under Westinghouse' s lead. The first of these programs carried out to completion was for Temelin NPP - VVER-1000 - in the first quarter of 2003. Other ongoing programs aim at providing a similar work for VVER-440 design, namely Dukovany, Mochovce and Bohunice NPPs. The challenge of adapting the existing generic WOG material to plants other than PWRs mainly arises for VVER-440 because of important differences in confinement design, making it more vulnerable to ex-vessel phenomena such as hydrogen burn. Also, for both eastern designs, cavity flooding strategy requires special consideration and adaptation on a case by case basis. The purpose of this paper is to provide some insights on how SAMG programs can be implemented for VVER-1000 and VVER-440 type of plants based on the WOG SAMG generic approach, and identifies areas of technical concerns encountered in the process. Solutions are proposed based on the experience of past and ongoing programs. (author)

  14. Echtzeitdetektion von Punktmutationen mit DNA-Chips am Beispiel des SULT1A1*213-SNP

    Gajovic-Eichelmann, Nenad; Griep, Andrea; Ehrentreich-Förster, Eva; Bier, Frank F

    2001-01-01

    Der Identifizierung von Punktmutationen im menschlichen Genom kommt eine hohe Bedeutung zu. Die Entdeckung einer Vielzahl von SNPs ('single nucleotide polymorphisms'), also Mutationen einzelner Basen, die definitionsgemäß bei mehr als 1% der Bevölkerung auftreten, und die Erkenntnis, dass SNPs die Nebenwirkungen von Medikamenten determinieren können, führte zu der Vision einer 'personalisierten Medizin': Der Patient erhält nach einer Genotypisierung das für ihn verträglichste Medikament versc...

  15. Pre-operational integrated pressure tests at EMO NPP (VVER 440/V 213) and options for their experimental utilization

    Pre-operational integrated leakage rate and structural integrity tests for the Mochovce NPP are discussed. A programme is presented of experiments proposed during these tests, to be used in investigations of the following phenomena: water drop entrainment from the water pool into the air trap; hydraulic resistance and leaktightness of check valve DN 500; performance of valves DN 250; response of bubble condenser walls to loading; phenomenology of the whole process and verification of some partial analytical and experimental procedures. (Z.S.) 2 figs

  16. First Results of Minimum Fisher Regularisation as Unfolding Method for JET NE213 Liquid Scintillator Neutron Spectrometry

    Mlynář, Jan; Adams, J. M.; Bertalot, L.; Conroy, S.

    2005-01-01

    Roč. 74, 1-4 (2005), s. 781-786. ISSN 0920-3796. [Symposium on Fusion Technology - SOFT/23rd./. Benátky, 20.9.2004-24.9.2004] Institutional research plan: CEZ:AV0Z20430508 Keywords : Tokamak * fusion * neutron diagnostic * spectrum unfolding * scintillator regularisation Subject RIV: BL - Plasma and Gas Discharge Physics Impact factor: 0.981, year: 2005 http://soft2004.igi.cnr.it/

  17. N-[2-(1,3-Benzodioxol-5-yl)eth­yl]-2-chloro­acetamide

    Dong, Hui-Chao

    2008-01-01

    The title compound, C11H12ClNO3, crystallizes with two independent mol­ecules in the asymmetric unit. Inter­molecular N—H⋯O hydrogen bonds link the mol­ecules related by translation along the b axis into two independent hydrogen-bonded chains. The crystal studied exhibited inversion twinning.

  18. PS2-13: Implementing a Lean Management System in Primary Care: Facilitators and Barriers from the Frontlines

    Hung, Dorothy; Gray, Caroline; Anderson, Katie; Hereford, James

    2013-01-01

    Background/Aims As approximately $750 billion is wasted in the U.S. health system each year, equivalent to roughly one-third of every medical dollar, “Lean” thinking and techniques offer promising solutions for maximizing value in health care. This study examines a large, multispecialty practice’s journey of implementing a Lean management system beginning in primary care. We sought to understand initial drivers and barriers to implementation, with lessons contributing to a learning system of improvement in health care. Methods This case study was based on in-depth interviews with 16 physician and administrative leaders, and 4 focus groups of medical assistants and administrative staff. Transcripts were coded and analyzed using inductive, grounded methodology. Results Respondents’ insights were clustered around three main themes: organizational leadership, professional values/culture, and availability of resources. Informants described organizational characteristics critical to implementing Lean and to which they attributed its success so far, including: strong leadership and the importance that leaders embody qualities they are espousing, willingness to engage all levels of staff in the change process, and willingness to adjust performance measures according to new job roles. However, many noted that values and norms surrounding clinical practice are often at odds with the Lean principle of standardizing work to eliminate waste, representing the biggest challenge for physicians who are socialized into a culture where independent thinking and autonomy is valued. The availability of resources was also cited as an important factor in executing changes, including time to do one’s regular work while implementing change, time to absorb new ideas and changes, and proper space configurations to support the change. Conclusions Lean represents a non-traditional approach to managing the delivery of medical care. In a Lean operating system, value is seen first from the patient perspective and while this is a point of easy agreement, how that principle is operationalized can be fraught with challenges that must be negotiated. These challenges may be addressed in part by strong leadership and adequate resources. Further study is currently underway as the effort is extended to additional sites in the organization, with additional findings to be presented on how Lean can be successfully implemented in health care.

  19. Development, erection, testing, and commissioning of the simulator for WWER-440 (W 213) nuclear power plant units

    A full-scope simulator has been developed and erected for the Greifswald NPP units 5 to 8. As derived from the main pedagogic objectives, the main properties of the simulator are described, selected information about engineering solution is presented, and experience with respect to development, erection, testing, and commissioning is given. (author)

  20. 47 CFR 68.213 - Installation of other than “fully protected” non-system simple customer premises wiring.

    2010-10-01

    ... protected” non-system simple customer premises wiring. (a) Scope of this rule. Provisions of this rule apply... symbol “CAT 3” or a symbol consisting of a “C” with a “3” contained within the “C” character,...

  1. 49 CFR 1546.213 - Access to cargo: Security threat assessments for cargo personnel in the United States.

    2010-10-01

    ... 49 Transportation 9 2010-10-01 2010-10-01 false Access to cargo: Security threat assessments for...: Security threat assessments for cargo personnel in the United States. This section applies in the United...— (1) Each individual must successfully complete a security threat assessment or comparable...

  2. Substituted 2,1,3-Benzothiadiazole- And Thiophene-Based Polymers for Solar Cells - Introducing a New Thermocleavable Precursor

    Petersen, Martin Helgesen; Gevorgyan, Suren; Krebs, Frederik C;

    2009-01-01

    performance than for the soluble precursor polymers; however, we found processing conditions that lead to a higher performance for the thermocleaved product, where open circuit voltages of up to 0.9 V could be obtained with power conversion efficiencies of up to 0.42%, representing a doubling as compared to...... thermocleavable materials have the advantage that they are insoluble after a thermal treatment, enabling a larger degree of processing freedom when preparing multilayer devices and they provide a better operational stability for the devices. So far the process of thermocleavage has led to poorer device...

  3. 2-(1,3-Benzothia­zol-2-yl)-6-eth­oxy­phenol

    Lakshmanan, D.; Raj, R. Madhan; Selvakumar, R; Bakthadoss, M.; Murugavel, S.

    2011-01-01

    In the title compound, C15H13NO2S, the benzothia­zole unit is essentially planar [maximum deviation = −0.0099 (5) Å for the S atom] and is oriented at a dihedral angle of 4.8 (5)° with respect to the benzene ring. An intra­molecular O—H⋯N hydrogen bond generates an S(6) ring motif. The crystal packing is stabilized by C—H⋯π inter­actions.

  4. Track 8: health and radiological applications. Isotopes and radiation: general. 3. Extraction of 229Th from 233U for Medical Research Applications

    The use of 213Bi as an alpha emitter is being explored by the medical research community for treatment of a variety of cancers. An example is the protocol for the treatment of acute myeloid leukemia developed by Memorial Sloan-Kettering Cancer Center. The humanized antibody HuM195 is used to target a surface protein on the cancer cell. Bismuth is linked to the antibody with a chelating agent. Because of the high linear energy transfer and short range of the emitted alpha particle, there is a high probability of killing the targeted cell without exposing other parts of the body to a large dose of radiation. Bismuth-213 is extracted as the decay product of 225Ac, which in turn is extracted as a decay product of 229Th. A limited supply of 229Th, itself a product of the decay of 233U, has been separated by Oak Ridge National Laboratory (ORNL). About 95 mCi was separated from waste materials remaining from the processing of 233U in the 1970's. An additional 15 mCi was separated directly from 233U in 1998. In June 2000, the secretary of energy announced a program to separate additional thorium to support Phase II human trials at Sloan-Kettering and growing research programs at other institutions. This paper describes a project that will extract an additional 70 mCi of 229Th from a 3.3-kg batch of 233U sent to ORNL by Mound Laboratories in 1996. This project requires a process and location that would support the sustained extraction of thorium from 233U. The flow-sheet used for the initial processing of uranium was adopted, with several modifications. The 233U is currently stored in the ORNL Radiochemical Development Facility (RDF). The adjacent Radioactive Materials Analytical Laboratory (RMAL) was selected as the processing site because of the availability of both trained radiochemical staff and the type of hot cells suitable for processing uranium with increasingly high levels of 232U. Decay products from this isotope, especially 208Tl, add to the dose associated with

  5. NRC review of Electric Power Research Institute`s advanced light water reactor utility requirements document. Passive plant designs, chapters 2-13, project number 669

    1994-08-01

    The Electric Power Research Institute (EPRI) is preparing a compendium of technical requirements, referred to as the {open_quotes}Advanced Light Water Reactor [ALWR] Utility Requirements Document{close_quotes}, that is acceptable to the design of an ALWR power plant. When completed, this document is intended to be a comprehensive statement of utility requirements for the design, construction, and performance of an ALWR power plant for the 1990s and beyond. The Requirements Document consists of three volumes. Volume I, {open_quotes}ALWR Policy and Summary of Top-Tier Requirements{close_quotes}, is a management-level synopsis of the Requirements Document, including the design objectives and philosophy, the overall physical configuration and features of a future nuclear plant design, and the steps necessary to take the proposed ALWR design criteria beyond the conceptual design state to a completed, functioning power plant. Volume II consists of 13 chapters and contains utility design requirements for an evolutionary nuclear power plant [approximately 1350 megawatts-electric (MWe)]. Volume III contains utility design requirements for nuclear plants for which passive features will be used in their designs (approximately 600 MWe). In April 1992, the staff of the Office of Nuclear Reactor Regulation, U.S. Nuclear Regulatory Commission, issued Volume 1 and Volume 2 (Parts 1 and 2) of its safety evaluation report (SER) to document the results of its review of Volumes 1 and 2 of the Requirements Document. Volume 1, {open_quotes}NRC Review of Electric Power Research Institute`s Advanced Light Water Reactor Utility Requirements Document - Program Summary{close_quotes}, provided a discussion of the overall purpose and scope of the Requirements Document, the background of the staff`s review, the review approach used by the staff, and a summary of the policy and technical issues raised by the staff during its review.

  6. In vitro screening for synergism of high-linear energy transfer Bi-213-radiotherapy with other therapeutic agents for the treatment of B-Cell chronic lymphocytic leukemia

    Vandenbulcke, Katia; Thierens, Hubert; De Vos, Filip; Philippe, Jan; Offner, Fritz; Janssens, Ann; Apostolidis, Christos; Morgenstern, Alfred; Bacher, Klaus; de Gelder, Virginie; Dierckx, Rudi A.; Slegers, Guido

    2006-01-01

    Background: External beam radiotherapy and beta-radioimmunotherapy (RIT) are effective treatments for lymphoid malignancies. The development of RIT with alpha-emitters is attractive, owing to the high (LET) nature and short path length of alpha particles allowing for higher tumor cell kill and lower

  7. 47 CFR 25.213 - Inter-Service coordination requirements for the 1.6/2.4 GHz mobile-satellite service.

    2010-10-01

    ... square root of (h) where h is the altitude of the aircraft in meters above ground level. (v) Smaller...), and (iv) of this section if agreed to by the Mobile-Satellite Service licensee and the Electromagnetic... radio astronomy service in the 1610.6-1613.8 MHz band against interference from 1.6/2.4 GHz...

  8. SU-C-213-04: Application of Depth Sensing and 3D-Printing Technique for Total Body Irradiation (TBI) Patient Measurement and Treatment Planning

    Purpose: To develop and validate an innovative method of using depth sensing cameras and 3D printing techniques for Total Body Irradiation (TBI) treatment planning and compensator fabrication. Methods: A tablet with motion tracking cameras and integrated depth sensing was used to scan a RANDOTM phantom arranged in a TBI treatment booth to detect and store the 3D surface in a point cloud (PC) format. The accuracy of the detected surface was evaluated by comparison to extracted measurements from CT scan images. The thickness, source to surface distance and off-axis distance of the phantom at different body section was measured for TBI treatment planning. A 2D map containing a detailed compensator design was calculated to achieve uniform dose distribution throughout the phantom. The compensator was fabricated using a 3D printer, silicone molding and tungsten powder. In vivo dosimetry measurements were performed using optically stimulated luminescent detectors (OSLDs). Results: The whole scan of the anthropomorphic phantom took approximately 30 seconds. The mean error for thickness measurements at each section of phantom compare to CT was 0.44 ± 0.268 cm. These errors resulted in approximately 2% dose error calculation and 0.4 mm tungsten thickness deviation for the compensator design. The accuracy of 3D compensator printing was within 0.2 mm. In vivo measurements for an end-to-end test showed the overall dose difference was within 3%. Conclusion: Motion cameras and depth sensing techniques proved to be an accurate and efficient tool for TBI patient measurement and treatment planning. 3D printing technique improved the efficiency and accuracy of the compensator production and ensured a more accurate treatment delivery

  9. Low cycle fatigue behaviour of neutron irradiated copper alloys at 250 and 350 deg. C. (ITER R and D Task no. T213)[International Thermonuclear Experimental Reactor

    Singh, B.N.; Stubbins, J.F. [Illinois Univ., Dept. of Nuclear Engineering, Illinois (United States); Toft, P

    2000-03-15

    The fatigue behaviour of a dispersion strengthened and a precipitation hardened copper alloys was investigated with and without irradiation exposure. Fatigue specimens of these alloys were irradiated with fission neutrons in the DR-3 reactor at Risoe with a flux of {approx}2.5 x 10{sup 17} n/m{sup 2}s (E> 1 MeV) to influence levels of 1.0 - 1.5 x 10{sup 24} n/m{sup 2} (E> 1 MeV) at 250 and 350 deg. C. These irradiations were carried out in temperature controlled rigs where the irradiation temperature was monitored and controlled continuously throughout the whole irradiation experiment. Both unirradiated and irradiated specimens were fatigue tested in vacuum at the irradiation temperatures of 250 and 350 deg. C in a strain controlled mode with a loading frequency of 0.5Hz. Post-fatigue microstructures were examined using transmission electron microscopy and the fracture surfaces were investigated using scanning electron microscope. The present investigations demonstrated that the fatigue life decreases with increasing temperature and that the exposure to neutron irradiation causes further degradation in fatigue life at both temperatures. These results are discussed in terms of the observed post-fatigue microstructures and the fracture surface morphology. Finally, the main conclusions and their implications are summarised. (au)

  10. SU-C-213-01: 3D Printed Patient Specific Phantom Composed of Bone and Soft Tissue Substitute Plastics for Radiation Therapy

    Ehler, E; Sterling, D; Higgins, P [University of Minnesota, Minneapolis, MN (United States)

    2015-06-15

    Purpose: 3D printed phantoms constructed of multiple tissue approximating materials could be useful in both clinical and research aspects of radiotherapy. This work describes a 3D printed phantom constructed with tissue substitute plastics for both bone and soft tissue; air cavities were included as well. Methods: 3D models of an anonymized nasopharynx patient were generated for air cavities, soft tissues, and bone, which were segmented by Hounsfield Unit (HU) thresholds. HU thresholds were chosen to define air-to-soft tissue boundaries of 0.65 g/cc and soft tissue-to-bone boundaries of 1.18 g/cc based on clinical HU to density tables. After evaluation of several composite plastics, a bone tissue substitute was identified as an acceptable material for typical radiotherapy x-ray energies, composed of iron and PLA plastic. PET plastic was determined to be an acceptable soft tissue substitute. 3D printing was performed on a consumer grade dual extrusion fused deposition model 3D printer. Results: MVCT scans of the 3D printed heterogeneous phantom were acquired. Rigid image registration of the patient and the 3D printed phantom scans was performed. The average physical density of the soft tissue and bone regions was 1.02 ± 0.08 g/cc and 1.39 ± 0.14 g/cc, respectively, for the patient kVCT scan. In the 3D printed phantom MVCT scan, the average density of the soft tissue and bone was 1.01 ± 0.09 g/cc and 1.44 ± 0.12 g/cc, respectively. Conclusion: A patient specific phantom, constructed of heterogeneous tissue substitute materials was constructed by 3D printing. MVCT of the 3D printed phantom showed realistic tissue densities were recreated by the 3D printing materials. Funding provided by intra-department grant by University of Minnesota Department of Radiation Oncology.

  11. SU-C-213-04: Application of Depth Sensing and 3D-Printing Technique for Total Body Irradiation (TBI) Patient Measurement and Treatment Planning

    Lee, M; Suh, T [Department of Biomedical Engineering, College of Medicine, The Catholic University of Korea, Seoul (Korea, Republic of); Research Institute of Biomedical Engineering, College of Medicine, The Catholic University of Korea, Seoul (Korea, Republic of); Han, B; Xing, L [Department of Radiation Oncology, Stanford University School of Medicine, Palo Alto, CA (United States); Jenkins, C [Department of Radiation Oncology, Stanford University School of Medicine, Palo Alto, CA (United States); Department of Mechanical Engineering, Stanford University, Palo Alto, CA (United States)

    2015-06-15

    Purpose: To develop and validate an innovative method of using depth sensing cameras and 3D printing techniques for Total Body Irradiation (TBI) treatment planning and compensator fabrication. Methods: A tablet with motion tracking cameras and integrated depth sensing was used to scan a RANDOTM phantom arranged in a TBI treatment booth to detect and store the 3D surface in a point cloud (PC) format. The accuracy of the detected surface was evaluated by comparison to extracted measurements from CT scan images. The thickness, source to surface distance and off-axis distance of the phantom at different body section was measured for TBI treatment planning. A 2D map containing a detailed compensator design was calculated to achieve uniform dose distribution throughout the phantom. The compensator was fabricated using a 3D printer, silicone molding and tungsten powder. In vivo dosimetry measurements were performed using optically stimulated luminescent detectors (OSLDs). Results: The whole scan of the anthropomorphic phantom took approximately 30 seconds. The mean error for thickness measurements at each section of phantom compare to CT was 0.44 ± 0.268 cm. These errors resulted in approximately 2% dose error calculation and 0.4 mm tungsten thickness deviation for the compensator design. The accuracy of 3D compensator printing was within 0.2 mm. In vivo measurements for an end-to-end test showed the overall dose difference was within 3%. Conclusion: Motion cameras and depth sensing techniques proved to be an accurate and efficient tool for TBI patient measurement and treatment planning. 3D printing technique improved the efficiency and accuracy of the compensator production and ensured a more accurate treatment delivery.

  12. SU-C-213-05: Evaluation of a Composite Copper-Plastic Material for a 3D Printed Radiation Therapy Bolus

    Vitzthum, L; Ehler, E; Sterling, D; Reynolds, T; Higgins, P; Dusenbery, K [University of Minnesota, Minneapolis, MN (United States)

    2015-06-15

    Purpose: To evaluate a novel 3D printed bolus fabricated from a copper-plastic composite as a thin flexible, custom fitting device that can replicate doses achieved with conventional bolus techniques. Methods: Two models of bolus were created on a 3D printer using a composite copper-PLA/PHA. Firstly, boluses were constructed at thicknesses of 0.4, 0.6 and 0.8 mm. Relative dose measurements were performed under the bolus with an Attix Chamber as well as with radiochromic film. Results were compared to superficial Attix Chamber measurements in a water equivalent material to determine the dosimetric water equivalence of the copper-PLA/PHA plastic. Secondly, CT images of a RANDO phantom were used to create a custom fitting bolus across the anterolateral scalp. Surface dose with the bolus placed on the RANDO phantom was measured with radiochromic film at tangential angles with 6, 10, 10 flattening filter free (FFF) and 18 MV photon beams. Results: Mean surface doses for 6, 10, 10FFF and 18 MV were measured as a percent of Dmax for the flat bolus devices of each thickness. The 0.4 mm thickness bolus was determined to be near equivalent to 2.5 mm depth in water for all four energies. Surface doses ranged from 59–63% without bolus and 85–90% with the custom 0.4 mm copper-plastic bolus relative to the prescribed dose for an oblique tangential beam arrangement on the RANDO phantom. Conclusion: Sub-millimeter thickness, 3D printed composite copper-PLA/PHA bolus can provide a build-up effect equivalent to conventional bolus. At this thickness, the 3D printed bolus allows a level of flexure that may provide more patient comfort than current 3D printing materials used in bolus fabrication while still retaining the CT based custom patient shape. Funding provided by an intra-department grant of the University of Minnesota Department of Radiation Oncology.

  13. 3,14-Diethyl-2,13-diaza-6,17-diazoniatricyclo[16.4.0.07,12]docosane dichloride tetrahydrate from synchrotron radiation

    Dohyun Moon

    2013-11-01

    Full Text Available The asymmetric unit of title hydrated salt, C22H46N42+·2Cl−·4H2O, comprises half a centrosymmetric dication, one Cl− anion and two water molecules of crystallization. The structure determination reveals that protonation has occurred at diagonally opposite amine N atoms, and that the dication features intramolecular N—H...N hydrogen bonds. In the crystal, a three-dimensional artchitecture is formed by O—H...Cl/N and N—H...Cl/O hydrogen bonds.

  14. SU-B-213-00: Education Council Symposium: Accreditation and Certification: Establishing Educational Standards and Evaluating Candidates Based on these Standards

    The North American medical physics community validates the education received by medical physicists and the clinical qualifications for medical physicists through accreditation of educational programs and certification of medical physicists. Medical physics educational programs (graduate education and residency education) are accredited by the Commission on Accreditation of Medical Physics Education Programs (CAMPEP), whereas medical physicists are certified by several organizations, the most familiar of which is the American Board of Radiology (ABR). In order for an educational program to become accredited or a medical physicist to become certified, the applicant must meet certain specified standards set by the appropriate organization. In this Symposium, representatives from both CAMPEP and the ABR will describe the process by which standards are established as well as the process by which qualifications of candidates for accreditation or certification are shown to be compliant with these standards. The Symposium will conclude with a panel discussion. Learning Objectives: Recognize the difference between accreditation of an educational program and certification of an individual Identify the two organizations primarily responsible for these tasks Describe the development of educational standards Describe the process by which examination questions are developed GS is Executive Secretary of CAMPEP

  15. NRC review of Electric Power Research Institute's advanced light water reactor utility requirements document. Passive plant designs, chapters 2-13, project number 669

    The Electric Power Research Institute (EPRI) is preparing a compendium of technical requirements, referred to as the open-quotes Advanced Light Water Reactor [ALWR] Utility Requirements Documentclose quotes, that is acceptable to the design of an ALWR power plant. When completed, this document is intended to be a comprehensive statement of utility requirements for the design, construction, and performance of an ALWR power plant for the 1990s and beyond. The Requirements Document consists of three volumes. Volume I, open-quotes ALWR Policy and Summary of Top-Tier Requirementsclose quotes, is a management-level synopsis of the Requirements Document, including the design objectives and philosophy, the overall physical configuration and features of a future nuclear plant design, and the steps necessary to take the proposed ALWR design criteria beyond the conceptual design state to a completed, functioning power plant. Volume II consists of 13 chapters and contains utility design requirements for an evolutionary nuclear power plant [approximately 1350 megawatts-electric (MWe)]. Volume III contains utility design requirements for nuclear plants for which passive features will be used in their designs (approximately 600 MWe). In April 1992, the staff of the Office of Nuclear Reactor Regulation, U.S. Nuclear Regulatory Commission, issued Volume 1 and Volume 2 (Parts 1 and 2) of its safety evaluation report (SER) to document the results of its review of Volumes 1 and 2 of the Requirements Document. Volume 1, open-quotes NRC Review of Electric Power Research Institute's Advanced Light Water Reactor Utility Requirements Document - Program Summaryclose quotes, provided a discussion of the overall purpose and scope of the Requirements Document, the background of the staff's review, the review approach used by the staff, and a summary of the policy and technical issues raised by the staff during its review

  16. PMK-2, the First Integral Thermal-Hydraulics Tests for the Safety Evaluation of VVER-440/213 Nuclear Power Plants

    Gy. Ézsöl

    2012-01-01

    Full Text Available The PMK-2 facility is a full-pressure thermal-hydraulic model of the primary and partly the secondary circuit of the VVER-type units of Paks NPP. The facility was the first integral-type facility for VVERs. The PMK-2 was followed later by the PACTEL (for VVER-440, the ISB, and PSB for VVER-1000. Since the startup of the facility in 1985, 55 experiments have been performed primarily in international frameworks with the participation of experts from 29 European and overseas countries forming a scientific school to better understand VVER system behaviour and reach a high level of modelling of accident sequences. The ATHLET, CATHARE, and RELAP5 codes have been validated including both qualitative and quantitative assessments. The former was almost exclusively applied to the early phase of validation by integral experiments, while the quantitative assessments have been performed by the Fast Fourier Transform Based Method. Paper gives comprehensive information on the design features of PMK-2 facility with a special respect to the representativeness of phenomena, the experiments performed, and the results of the validation of ATHLET, CATHARE, and RELAP5 codes. Safety significance of the PMK-2 projects is also discussed.

  17. Radio observations of the TeV source HESS J1943+213: a new case of a pulsar wind nebula?

    Gabanyi, K E; Giacani, E; Paragi, Z; Pidopryhora, Y; Frey, S

    2011-01-01

    Recently, the H.E.S.S. Collaboration discovered a very high energy gamma-ray point source close to the Galactic plane. They offered three possible explanations for the nature of the source: a gamma-ray binary, a pulsar wind nebula, or a BL Lac object. They concluded that the observations favoured an extreme BL Lac object interpretation. We investigated the nature of the radio source reported as the counterpart of the very high energy gamma-ray source. We performed high-resolution radio interferometric observations with the European Very Long Baseline Interferometry Network at a frequency of 1.6 GHz on 2011 May 18. We also reanalysed archival 1.4-GHz radio continuum and HI spectral line data taken with the Very Large Array. The accurate position of the radio source, as observed with EVN, is ~ 4" off from the one obtained in the NRAO VLA Sky Survey. The new position is in excellent agreement with that of the proposed X-ray counterpart of the TeV source. From HI absorption data, a distance of about 11.5 +/- 1.5 ...

  18. SU-D-213-04: Accounting for Volume Averaging and Material Composition Effects in An Ionization Chamber Array for Patient Specific QA

    Purpose: This study explores novel methods to address two significant challenges affecting measurement of patient-specific quality assurance (QA) with IBA’s Matrixx Evolution™ ionization chamber array. First, dose calculation algorithms often struggle to accurately determine dose to the chamber array due to CT artifact and algorithm limitations. Second, finite chamber size and volume averaging effects cause additional deviation from the calculated dose. Methods: QA measurements were taken with the Matrixx positioned on the treatment table in a solid-water Multi-Cube™ phantom. To reduce the effect of CT artifact, the Matrixx CT image set was masked with appropriate materials and densities. Individual ionization chambers were masked as air, while the high-z electronic backplane and remaining solid-water material were masked as aluminum and water, respectively. Dose calculation was done using Varian’s Acuros XB™ (V11) algorithm, which is capable of predicting dose more accurately in non-biologic materials due to its consideration of each material’s atomic properties. Finally, the exported TPS dose was processed using an in-house algorithm (MATLAB) to assign the volume averaged TPS dose to each element of a corresponding 2-D matrix. This matrix was used for comparison with the measured dose. Square fields at regularly-spaced gantry angles, as well as selected patient plans were analyzed. Results: Analyzed plans showed improved agreement, with the average gamma passing rate increasing from 94 to 98%. Correction factors necessary for chamber angular dependence were reduced by 67% compared to factors measured previously, indicating that previously measured factors corrected for dose calculation errors in addition to true chamber angular dependence. Conclusion: By comparing volume averaged dose, calculated with a capable dose engine, on a phantom masked with correct materials and densities, QA results obtained with the Matrixx Evolution™ can be significantly improved. In addition, necessary correction factors are reduced, allowing for more reliable and meaningful patient-specific QA measurements

  19. Integrated Plant Safety Assessment: Systematic Evaluation Program. Haddam Neck Plant, Connecticut Yankee Atomic Power Company, Docket No. 50-213. Final report

    The Systematic Evaluation Progam was initiated in February 1977 by the US Nuclear Regulatory Commission review the designs of older operating nuclear reactor plants to confirm and document their safety. The review provides: (1) an assessment of how these plants compare with curent licensing safety requirements relating to selected issues, (2) a basis for deciding on how these differences should be resolved in an integrated plant review, and (3) a documented evaluation of plant safety. This report documents the review of Haddam Neck Plant, operated by Connecticut Yankee Atomic Power Company. The Haddam Neck Plant is one of 10 plants reviewed under Phase II of this program. This report indicates how 137 topics selected for review under Phase I of the program were addressed. Equipment and procedural changes have been identified as a result of the review

  20. �Return to the Lord your God, for he is gracious and compassionate...� (Jl 2:13. A prophetic perspective on reconciliation and restoration

    W J Wessels

    2005-10-01

    Full Text Available In this article the Old Testament prophetic literature will be the focus of discussion with regard to the matter of restoring broken relationships, be it between God and humans or between humans as such. The relationship between Yahweh and his people was formally established by means of a covenant. The presentation of the prophetic material is done with a narrow focus on the issues of reconciliation and restoration. Prophets and prophetic� texts are selected with the mentioned focus in mind and presented as the results of research done on the chosen texts. It is therefore done with a real awareness of the complexity of the prophetic material and the historical embedding of prophetic material. A detailed discussion of introductory and theological issues are therefore not presented, but taken into account. What result in this paper is a focussed presentation on the mentioned topics in some of the prophetic material. Although the word reconciliation is not an Old Testament concept, it is clear that the idea of re-establishment and renewal of� the relationship between God and people was ever present. The prophets had the duty to address the reasons for damage to this covenant relationship and to warn the people of the consequences if they continue to disobey the stipulations and ethical demands of this relationship. The prophets not only served as witnesses of the efforts of Yahweh to reconcile with his people, but also as instruments to bring it about.�

  1. SU-B-213-00: Education Council Symposium: Accreditation and Certification: Establishing Educational Standards and Evaluating Candidates Based on these Standards

    NONE

    2015-06-15

    The North American medical physics community validates the education received by medical physicists and the clinical qualifications for medical physicists through accreditation of educational programs and certification of medical physicists. Medical physics educational programs (graduate education and residency education) are accredited by the Commission on Accreditation of Medical Physics Education Programs (CAMPEP), whereas medical physicists are certified by several organizations, the most familiar of which is the American Board of Radiology (ABR). In order for an educational program to become accredited or a medical physicist to become certified, the applicant must meet certain specified standards set by the appropriate organization. In this Symposium, representatives from both CAMPEP and the ABR will describe the process by which standards are established as well as the process by which qualifications of candidates for accreditation or certification are shown to be compliant with these standards. The Symposium will conclude with a panel discussion. Learning Objectives: Recognize the difference between accreditation of an educational program and certification of an individual Identify the two organizations primarily responsible for these tasks Describe the development of educational standards Describe the process by which examination questions are developed GS is Executive Secretary of CAMPEP.

  2. A Political Decision Disguised as Legal Argument? Opinion 2/13 and European Union Accession to the European Convention on Human Rights

    Butler, Graham

    2015-01-01

    David Thór Björgvinsson was a judge of the European Court of Human Rights between 2004 and 2013. During this period, he was involved in many important judgments, including Scoppola v Italy (No. 3), Eweida and others v United Kingdom, and Al-Jedda v the United Kingdom, amongst others, and went on ...

  3. Superfund record of decision (EPA Region 10): Idaho National Engineering Laboratory (USDOE), Operable Unit 2-13, Idaho Falls, ID, December 17, 1997

    The Test Reactor Area (TRA) Waste Area Group (WAG) 2 is one of the ten Idaho National Engineering and Environmental Laboratory (INEEL) WAGs identified in the Federal Facilities Agreement and Consent Order (FFA/CO) by the US Environmental Protection Agency (EPA) Region 10, the Idaho Department of Health and Welfare (IDHW), and the US Department of Energy (DOE). This resulting comprehensive Record of Decision (ROD) document presents the selected remedial actions for eight contaminant release sites at the TRA of the INEEL, Idaho Falls, Idaho. It provides information to support remedial actions for these eight sites where contamination presents an unacceptable risk, and a ''No Action'' decision on 47 additional sites at the TRA

  4. X-linked mental retardation with thin habitus, osteoporosis, and kyphoscoliosis: Linkage to Xp21.3-p22.12

    Arena, J.F.; Lubs, H. [Univ. of Miami School of Medicine, Miami, FL (United States); Schwartz, C. [Greenwood Genetic Center, SC (United States)] [and others

    1996-07-12

    We reevaluated a family previously described as having nonspecific X-linked mental retardation (XLMR) by Snyder and Robinson (MINI 309583). Clinical and DNA studies were conducted on 17 relatives, including 6 males with mild-to-moderate mental retardation, 3 carrier females, and 8 normal males. In contrast to the normal appearance and minimal clinical findings reported 22 years ago, affected males were found to have a characteristic set of clinical findings. These developed gradually over the first 2 decades, and included thin body build with diminished muscle mass, osteoporosis and kyphoscoliosis, slight facial asymmetry with a prominent lower lip, nasal speech, high narrow or cleft palate, and long great toes. Carrier females were clinically normal. Multipoint linkage analysis indicated linkage to markers distal to the 3{prime} end of DMD (DXS41 and DXS989), with a maximal lod score of 4.7. On the basis of these findings, this entity is redefined as XLMR syndrome. 22 refs., 6 figs., 2 tabs.

  5. Lifestyle and the importance of health education in the cardiac rehabilitation after myocardial revascularization surgery - doi:10.5020/18061230.2007.p213

    Denise Gonçaleves Moura Pinheiro

    2012-01-01

    Full Text Available In the treatment of ischemic cardiopathy, the prevention has a main role and the modifications in the lifestyle are indispensable for the good prognosis of the disease. The goal of the study was to describe the lifestyle regarding the prevalence of cardiovascular risk factors, such as smoking, alcohol consumption, dietary habits and sedentary behaviors before myocardial revascularization surgery and during the period of cardiac rehabilitation in a private institution that did not comprise structured health education activities. This was a retrospective, observational study, with a qualitative approach, held with 50 patients submitted to cardiac rehabilitation (36 men and 14 women; age 61±12.74 years. The data were collected from clinical records of the pre-cardiac rehabilitation evaluation which consisted of clinical data and information referring to the patients’ lifestyle. Amongst the most prevalent co-morbidities in the sample, there were: the hypertension (n=24; 48%, the diabetes mellitus (n=18; 36% and dyslipidemias (n=17; 34%. A high rate of smoke cessation (100% and 58% of sedentary behaviors (n=29 was observed after the cardiac surgery. This same number (n=29; 58% referred to have adhered to changes in dietary habits after the myocardial acute infarct. There was also an increase in the prevalence of alcohol consumption (n=21; 42% after myocardial revascularization. We conclude with this research that a cardiac rehabilitation program should provide to their patients, health education actions, for a necessary and real change in lifestyle habits, with the presence of a multidisciplinary team.

  6. SU-D-213-04: Accounting for Volume Averaging and Material Composition Effects in An Ionization Chamber Array for Patient Specific QA

    Fugal, M; McDonald, D; Jacqmin, D; Koch, N; Ellis, A; Peng, J; Ashenafi, M; Vanek, K [Medical University of South Carolina, Charleston, SC (United States)

    2015-06-15

    Purpose: This study explores novel methods to address two significant challenges affecting measurement of patient-specific quality assurance (QA) with IBA’s Matrixx Evolution™ ionization chamber array. First, dose calculation algorithms often struggle to accurately determine dose to the chamber array due to CT artifact and algorithm limitations. Second, finite chamber size and volume averaging effects cause additional deviation from the calculated dose. Methods: QA measurements were taken with the Matrixx positioned on the treatment table in a solid-water Multi-Cube™ phantom. To reduce the effect of CT artifact, the Matrixx CT image set was masked with appropriate materials and densities. Individual ionization chambers were masked as air, while the high-z electronic backplane and remaining solid-water material were masked as aluminum and water, respectively. Dose calculation was done using Varian’s Acuros XB™ (V11) algorithm, which is capable of predicting dose more accurately in non-biologic materials due to its consideration of each material’s atomic properties. Finally, the exported TPS dose was processed using an in-house algorithm (MATLAB) to assign the volume averaged TPS dose to each element of a corresponding 2-D matrix. This matrix was used for comparison with the measured dose. Square fields at regularly-spaced gantry angles, as well as selected patient plans were analyzed. Results: Analyzed plans showed improved agreement, with the average gamma passing rate increasing from 94 to 98%. Correction factors necessary for chamber angular dependence were reduced by 67% compared to factors measured previously, indicating that previously measured factors corrected for dose calculation errors in addition to true chamber angular dependence. Conclusion: By comparing volume averaged dose, calculated with a capable dose engine, on a phantom masked with correct materials and densities, QA results obtained with the Matrixx Evolution™ can be significantly improved. In addition, necessary correction factors are reduced, allowing for more reliable and meaningful patient-specific QA measurements.

  7. Low cycle fatigue behaviour of neutron irradiated copper alloys at 250 and 350 deg. C. (ITER R and D Task no. T213)

    The fatigue behaviour of a dispersion strengthened and a precipitation hardened copper alloys was investigated with and without irradiation exposure. Fatigue specimens of these alloys were irradiated with fission neutrons in the DR-3 reactor at Risoe with a flux of ∼2.5 x 1017 n/m2s (E> 1 MeV) to influence levels of 1.0 - 1.5 x 1024 n/m2 (E> 1 MeV) at 250 and 350 deg. C. These irradiations were carried out in temperature controlled rigs where the irradiation temperature was monitored and controlled continuously throughout the whole irradiation experiment. Both unirradiated and irradiated specimens were fatigue tested in vacuum at the irradiation temperatures of 250 and 350 deg. C in a strain controlled mode with a loading frequency of 0.5Hz. Post-fatigue microstructures were examined using transmission electron microscopy and the fracture surfaces were investigated using scanning electron microscope. The present investigations demonstrated that the fatigue life decreases with increasing temperature and that the exposure to neutron irradiation causes further degradation in fatigue life at both temperatures. These results are discussed in terms of the observed post-fatigue microstructures and the fracture surface morphology. Finally, the main conclusions and their implications are summarised. (au)

  8. Final report on characterization of physical and mechanical properties of copper and copper alloys before and after irradiation. (ITER R and D Task no. T213)

    The present report summarizes and highlights the main results of the work carried out during the last 5 - 6 years on effects of neutron irradiation on physical and mechanical properties of copper and copper alloys. The work was an European contribution to ITER Research and Development programme and was carried out by the Associations Euratom - Risoe and Euratom - Tekes. Details of the investigations carried out within the framework of the present task and the main results have been reported in various reports and journal publication. On the basis of these results some conclusions are drawn regarding the suitability of a copper alloy for its use in the first wall and divertor components of ITER. It is pointed out that the present work has managed only to identify some of the critical problems and limitations of the copper alloys for their employment in the hostile environment of 14 MeV neutrons. A considerable amount of further effort is needed to find a realistic and optimum solution. (au)

  9. Validation of six-loop RELAP5 model of VVER-440/V213 unit with transients measured in the Paks NPP

    In the AGNES project a six-loop input model of then third unit of the Paks NPP has been developed for the RELAP5/mod2 computer code. To verify that model, transients measured in Paks NPP were chosen for simulation: trip of main circulating pump and scram after a spurious signal from the safety system. The analyses of the transients show that, with only a very few modifications in the input model, the results are in a good agreement with the measured data. (author)

  10. μ-Acetato-aqua-μ-(5-bromo-2-{1,3-bis[2-(5-bromo-2-oxidobenzylideneaminoethyl]imidazolidin-2-yl}phenolatomethanoldinickel(II methanol disolvate monohydrate

    Yilma Gultneh

    2011-10-01

    Full Text Available The crystal structure of the title compound, [Ni2(C27H24Br3N4O3(CH3CO2(CH3OH(H2O]·2CH3OH·H2O contains [L(OAc{(CH3OHNi}{(H2ONi}] molecules {H3L = 2-(5-bromo-2-hydroxyphenyl-1,3-bis[4-(5-bromo-2-hydroxyphenyl-3-azabut-3-enyl]-1,3-imidazolidine} with additional water and two methanol solvent molecules. In this instance, one of the two Ni atoms is coordinated to a water and the other to a methanol molecule. The Ni—O and Ni—N distances, as well as the angles about the metal atoms, show quite regular octahedra around the central ions. The Ni—Ophenol—Ni and Ni—Oacetate—Ni angles are not similar [95.26 (13 and 97.34 (13°, respectively], indicating that this subtle solvate exchange induces significant differences in the conformation adopted. The coordinated methanol ligand is involved in an intramolecular hydrogen bond to the uncoordinated O atom of the bridging acetate ligand, while the coordinated water molecule forms a hydrogen bond with the one of the methanol solvent molecules. The water solvent molecule forms strong hydrogen bonds to both phenolate O atoms. The remaining methanol solvent molecule also forms a hydrogen bond with this solvent water molecule.

  11. SU-D-213-05: Design, Evaluation and First Applications of a Off-Site State-Of-The-Art 3D Dosimetry System

    Purpose: To design, construct and commission a prototype in-house three dimensional (3D) dose verification system for stereotatic body radiotherapy (SBRT) verification at an off-site partner institution. To investigate the potential of this system to achieve sufficient performance (1mm resolution, 3% noise, within 3% of true dose reading) for SBRT verification. Methods: The system was designed utilizing a parallel ray geometry instigated by precision telecentric lenses and an LED 630nm light source. Using a radiochromic dosimeter, a 3D dosimetric comparison with our gold-standard system and treatment planning software (Eclipse) was done for a four-field box treatment, under gamma passing criteria of 3%/3mm/10% dose threshold. Post off-site installation, deviations in the system’s dose readout performance was assessed by rescanning the four-field box irradiated dosimeter and using line-profiles to compare on-site and off-site mean and noise levels in four distinct dose regions. As a final step, an end-to-end test of the system was completed at the off-site location, including CT-simulation, irradiation of the dosimeter and a 3D dosimetric comparison of the planned (Pinnacle3) to delivered dose for a spinal SBRT treatment(12 Gy per fraction). Results: The noise level in the high and medium dose regions of the four field box treatment was relatively 5% pre and post installation. This reflects the reduction in positional uncertainty through the new design. This At 1mm dose voxels, the gamma pass rates(3%,3mm) for our in-house gold standard system and the off-site system were comparable at 95.8% and 93.2% respectively. Conclusion: This work will describe the end-to-end process and results of designing, installing, and commissioning a state-of-the-art 3D dosimetry system created for verification of advanced radiation treatments including spinal radiosurgery

  12. Safety analysis of an accident at a WWER-440/213 NPP associated with uncontrolled opening of the pressurizer relief valve

    A LOCA due to uncontrolled opening of the pressurizer relief valve with an equivalent diameter of 46 mm was analyzed for the Dukovany NPP using both the old and the new fuel design. The changes in the fuel design were found to have no appreciable effect on the accident. Although the conservative assumptions were more stringent that as corresponds to the design basis principle, the limiting cladding temperature and reactor core exposure values were not exceeded during the period analyzed (4000 s). The existing emergency core cooling system, comprising 1 high-pressure pump, 2 hydroaccumulators, and 1 low-pressure pump, was found to be efficient enough to ensure sufficient cooling of the reactor core. 3 tabs., 183 figs., 10 refs

  13. SU-D-213-03: Towards An Optimized 3D Scintillation Dosimetry Tool for Quality Assurance of Dynamic Radiotherapy Techniques

    Rilling, M [Département de physique, de génie physique et d’optique, Université Laval, Quebec City, QC (Canada); Centre de Recherche sur le Cancer, Hôtel-Dieu de Québec, Quebec City, QC (Canada); Département de radio-oncologie, CHU de Québec, Quebec City, QC (Canada); Center for Optics, Photonics and Lasers, Université Laval, Quebec City, QC, CA (Canada); Goulet, M [Département de radio-oncologie, CHU de Québec, Quebec City, QC (Canada); Thibault, S [Département de physique, de génie physique et d’optique, Université Laval, Quebec City, QC (Canada); Center for Optics, Photonics and Lasers, Université Laval, Quebec City, QC, CA (Canada); Archambault, L [Département de physique, de génie physique et d’optique, Université Laval, Quebec City, QC (Canada); Centre de Recherche sur le Cancer, Hôtel-Dieu de Québec, Quebec City, QC (Canada); Département de radio-oncologie, CHU de Québec, Quebec City, QC (Canada)

    2015-06-15

    Purpose: The purpose of this work is to simulate a multi-focus plenoptic camera used as the measuring device in a real-time three-dimensional scintillation dosimeter. Simulating and optimizing this realistic optical system will bridge the technological gap between concept validation and a clinically viable tool that can provide highly efficient, accurate and precise measurements for dynamic radiotherapy techniques. Methods: The experimental prototype, previously developed for proof of concept purposes, uses an off-the-shelf multi-focus plenoptic camera. With an array of interleaved microlenses of different focal lengths, this camera records spatial and angular information of light emitted by a plastic scintillator volume. The three distinct microlens focal lengths were determined experimentally for use as baseline parameters by measuring image-to-object magnification for different distances in object space. A simulated plenoptic system was implemented using the non-sequential ray tracing software Zemax: this tool allows complete simulation of multiple optical paths by modeling interactions at interfaces such as scatter, diffraction, reflection and refraction. The active sensor was modeled based on the camera manufacturer specifications by a 2048×2048, 5 µm-pixel pitch sensor. Planar light sources, simulating the plastic scintillator volume, were employed for ray tracing simulations. Results: The microlens focal lengths were determined to be 384, 327 and 290 µm. A realistic multi-focus plenoptic system, with independently defined and optimizable specifications, was fully simulated. A f/2.9 and 54 mm-focal length Double Gauss objective was modeled as the system’s main lens. A three-focal length hexagonal microlens array of 250-µm thickness was designed, acting as an image-relay system between the main lens and sensor. Conclusion: Simulation of a fully modeled multi-focus plenoptic camera enables the decoupled optimization of the main lens and microlens specifications. This work leads the way to improving the 3D dosimeter’s achievable resolution, efficiency and build for providing a quality assurance tool fully meeting clinical needs. M.R. is financially supported by a Master’s Canada Graduate Scholarship from the NSERC. This research is also supported by the NSERC Industrial Research Chair in Optical Design.

  14. 3p21.3 tumor suppressor gene RBM5 inhibits growth of human prostate cancer PC-3 cells through apoptosis

    Zhao Lijing

    2012-11-01

    Full Text Available Abstract Background Recent studies have indicated that the nuclear RNA-binding protein RBM5 has the ability to modulate apoptosis and suppress tumor growth. The aim of this study is to investigate the expression of RBM5 in human prostate cancer and its mechanism of tumor suppression. Methods The expression of RBM5 protein in cancerous prostatic tissues and normal tissues was examined by IHC. PC-3 cell line was used to determine the apoptotic function of RBM5 in vitro. PC-3 cells were transiently transfected with pcDNA3.1-RBM5. Cell viability was determined by MTT assay. Rhodamine 123 staining and Annexin V analysis were performed to observe the apoptotic activity of PC-3 cells overexpressing RBM5. Expression of apoptosis-related genes was assessed by western blot. Results The expression of RBM5 protein was significantly decreased in cancerous prostatic tissues compared to the normal tissues. PC-3 cells overexpressing RBM5 showed not only significant growth inhibition compared with the vector controls, but also dysfunction of mitochondrial membrane potential and increased apoptotic activity. To further define RBM5 function in apoptotic pathways, we investigated differential expression profiles of various BH3-only proteins including Bid, Bad, and Bim, and apoptosis regulatory proteins include P53, cleaved caspase9, and cleaved caspase3. We found that the expression of both BH3-only proteins and apoptosis regulatory proteins was increased in RBM5 transfected cells. Conclusion The expression of RBM5 protein was significantly decreased in cancerous prostatic tissues, which suggests that RBM5 plays an important role in the pathogenesis of prostate cancer. RBM5 may induce the apoptosis of prostate cancer PC-3 cells by modulating the mitochondrial apoptotic pathway, and thus RBM5 might be a promising target for gene therapy on prostate cancer.

  15. SU-E-T-213: Initial Experience with VMAT Plan and Delivery Verification Using a DICOM-RT Framework and Linac Delivery Log Files

    Reynolds, R; Pompos, A; Gu, X; Jiang, S; Stojadinovic, S [UT Southwestern Medical Center, Dallas, TX (United States)

    2014-06-01

    Purpose: Mobius3D/MobiusFX (M3D/MFX), a commercial DICOM-RT based plan and delivery verification system, was used to compare calculated and delivered volumetric modulated arc therapy (VMAT) dose distributions using TrueBeam delivery log files (TrajectoryLogs). Methods: M3D/MFX utilizes measured linac commissioning data to generate institution specific beam models for evaluating planned and delivered dose distributions. 30 RapidArc prostate plans and 30 head and neck SmartArc plans were used in this study. For every plan, CT images, contoured structure sets, RT-plan, and RT-dose files were exported to M3D, which recalculated the patients’ planning CT dose distributions using a collapsed-cone-convolutionsuperposition algorithm. MFX utilized the acquired TrajectoryLogs to compute patients’ delivered dose distributions based on actual treatment delivery parameters. The agreement between computed and delivered dose distributions was evaluated utilizing a (3%, 3mm) global 3D-gamma analysis and dose-volume histogram changes for targets and organs at risk. Results: Excellent 3D-gamma agreements were observed for all VMAT plans. On average, for computed and delivered RapidArc and SmartArc plans the gamma passing rates were (99.0%±1.4%) and (96.8%±1.8%), respectively. The average difference for primary target prescription dose percent-coverage between calculated and delivered plans was (− 0.09%±2.52%) for RapidArc and (−2.71%±4.62%) for SmartArc cases. Similarly, the planning target mean dose differences were (1.38%±0.96%) for RapidArc and (1.17%±0.72%) for SmartArc plans. For the prostate plans, the calculated and delivered variations of the maximum dose for a 2cc volume for bladder and rectum were (1.32%±1.26%) and (0.65%±1.44%), respectively. The spinal-cord 2cc maximum dose differences of (3.26%±1.68%) were observed for the SmartArc plans. Conclusions: Clinical quality assurance practice based on linac treatment log files for verification of delivered 3D dose distributions in the patients’ geometries represents a paradigm shift from dose measurements in a phantom. This approach captures treatment planning beam modeling differences as well as the linac uncertainties during treatment delivery of the plan.

  16. SU-E-T-213: Initial Experience with VMAT Plan and Delivery Verification Using a DICOM-RT Framework and Linac Delivery Log Files

    Purpose: Mobius3D/MobiusFX (M3D/MFX), a commercial DICOM-RT based plan and delivery verification system, was used to compare calculated and delivered volumetric modulated arc therapy (VMAT) dose distributions using TrueBeam delivery log files (TrajectoryLogs). Methods: M3D/MFX utilizes measured linac commissioning data to generate institution specific beam models for evaluating planned and delivered dose distributions. 30 RapidArc prostate plans and 30 head and neck SmartArc plans were used in this study. For every plan, CT images, contoured structure sets, RT-plan, and RT-dose files were exported to M3D, which recalculated the patients’ planning CT dose distributions using a collapsed-cone-convolutionsuperposition algorithm. MFX utilized the acquired TrajectoryLogs to compute patients’ delivered dose distributions based on actual treatment delivery parameters. The agreement between computed and delivered dose distributions was evaluated utilizing a (3%, 3mm) global 3D-gamma analysis and dose-volume histogram changes for targets and organs at risk. Results: Excellent 3D-gamma agreements were observed for all VMAT plans. On average, for computed and delivered RapidArc and SmartArc plans the gamma passing rates were (99.0%±1.4%) and (96.8%±1.8%), respectively. The average difference for primary target prescription dose percent-coverage between calculated and delivered plans was (− 0.09%±2.52%) for RapidArc and (−2.71%±4.62%) for SmartArc cases. Similarly, the planning target mean dose differences were (1.38%±0.96%) for RapidArc and (1.17%±0.72%) for SmartArc plans. For the prostate plans, the calculated and delivered variations of the maximum dose for a 2cc volume for bladder and rectum were (1.32%±1.26%) and (0.65%±1.44%), respectively. The spinal-cord 2cc maximum dose differences of (3.26%±1.68%) were observed for the SmartArc plans. Conclusions: Clinical quality assurance practice based on linac treatment log files for verification of delivered 3D dose distributions in the patients’ geometries represents a paradigm shift from dose measurements in a phantom. This approach captures treatment planning beam modeling differences as well as the linac uncertainties during treatment delivery of the plan

  17. Caloric Intake from Fast Food among Children and Adolescents in the United States, 2011-2012. NCHS Data Brief. Number 213

    Vikraman, Sundeep; Fryar, Cheryl D.; Ogden, Cynthia L.

    2015-01-01

    Consumption of fast food has been linked to weight gain in adults. Fast food has also been associated with higher caloric intake and poorer diet quality in children and adolescents. From 1994 through 2006, caloric intake from fast food increased from 10% to 13% among children aged 2-18 years. This report presents the most recent data on the…

  18. Improved HPLC-ECD analysis of mitomycin C, porfiromycin, VP 16-213 and VM 26 by implantation of software filters.

    Ploegmakers, H H; Mertens, M J; van Oort, W J

    1987-01-01

    In high performance liquid chromatography with electrochemical detection (HPLC/ECD) much attention has been paid to the performance of the applied detection system with respect to reliability and sensitivity. In general, insufficient attention has been paid to relatively easy to implant devices for improved collection and handling of detection signals. Four models of software filtering are studied and compared with hardware filtering. In the investigated chromatographic-electrochemical system, off-line parabolic filtering after on-line averaging of sixteen measurements proved to be the system of first choice, with respect to execution time, noise level, signal to noise ratio, peak height and resolution. PMID:3126702

  19. Targeted alpha-therapy using [Bi-213]anti-CD20 as novel treatment option for radio- and chemoresistant non-Hodgkin lymphoma cells

    Roscher, Mareike; Hormann, Inis; Leib, Oliver; Marx, Sebastian; Moreno, Josue; Miltner, Erich; Friesen, Claudia

    2013-01-01

    Radioimmunotherapy (RIT) is an emerging treatment option for non-Hodgkin lymphoma (NHL) producing higher overall response and complete remission rates compared with unlabelled antibodies. However, the majority of patients treated with conventional or myeloablative doses of radiolabelled antibodies relapse. The development of RIT with alpha-emitters is attractive for a variety of cancers because of the high linear energy transfer (LET) and short path length of alpha-radiation in human tissue, ...

  20. Effect of post-irradiation heat treatment on mechanical properties of OFHC-copper and copper alloys. (ITER R and D Task No. 213)

    The present report describes the results of post-irradiation annealing experiments carried out to test the possibility as to whether or not such treatments can be used to improve the ductility of copper alloys irradiated and tested at temperatures below the recovery stage V. Tensile specimens of OFHC-copper, CuCrZr, CuNiBe, CuAl-25 and CuAl-60 were irradiated with fission neutrons in the DR-3 reactor at Risoe at 100 deg. C to different displacement dose levels. Some of the specimens were tested in the as-irradiated conditions whereas others were given a post-irradiation heat treatment (PIHT) at 300 deg. C for 50 h and then tested at 100 deg. C. The results show that PIHT leads to only a partial recovery and that the level of recovery depends on the irradiation dose level. It is important to note, however, that the PIHT does eliminate the problem of yield drop and plastic instability and does reinstate enough of the uniform elongation to render the material useful again. It is suggested that more systematic investigations are necessary to determine whether or not this advantage can be maintained if the materials were to experience such cycles of irradiation and annealing repeatedly. (au)