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Sample records for wimp line sensitivity

  1. Improved EDELWEISS-III sensitivity for low-mass WIMPs using a profile likelihood approach

    Energy Technology Data Exchange (ETDEWEB)

    Hehn, L. [Karlsruher Institut fuer Technologie, Institut fuer Kernphysik, Karlsruhe (Germany); Armengaud, E.; Boissiere, T. de; Gros, M.; Navick, X.F.; Nones, C.; Paul, B. [CEA Saclay, DSM/IRFU, Gif-sur-Yvette Cedex (France); Arnaud, Q. [Univ Lyon, Universite Claude Bernard Lyon 1, CNRS/IN2P3, Institut de Physique Nucleaire de Lyon, Lyon (France); Queen' s University, Kingston (Canada); Augier, C.; Billard, J.; Cazes, A.; Charlieux, F.; Jesus, M. de; Gascon, J.; Juillard, A.; Queguiner, E.; Sanglard, V.; Vagneron, L. [Univ Lyon, Universite Claude Bernard Lyon 1, CNRS/IN2P3, Institut de Physique Nucleaire de Lyon, Lyon (France); Benoit, A.; Camus, P. [Institut Neel, CNRS/UJF, Grenoble (France); Berge, L.; Chapellier, M.; Dumoulin, L.; Giuliani, A.; Le-Sueur, H.; Marnieros, S.; Olivieri, E.; Poda, D. [CSNSM, Univ. Paris-Sud, CNRS/IN2P3, Universite Paris-Saclay, Orsay (France); Bluemer, J. [Karlsruher Institut fuer Technologie, Institut fuer Kernphysik, Karlsruhe (Germany); Karlsruher Institut fuer Technologie, Institut fuer Experimentelle Kernphysik, Karlsruhe (Germany); Broniatowski, A. [CSNSM, Univ. Paris-Sud, CNRS/IN2P3, Universite Paris-Saclay, Orsay (France); Karlsruher Institut fuer Technologie, Institut fuer Experimentelle Kernphysik, Karlsruhe (Germany); Eitel, K.; Kozlov, V.; Siebenborn, B. [Karlsruher Institut fuer Technologie, Institut fuer Kernphysik, Karlsruhe (Germany); Foerster, N.; Heuermann, G.; Scorza, S. [Karlsruher Institut fuer Technologie, Institut fuer Experimentelle Kernphysik, Karlsruhe (Germany); Jin, Y. [Laboratoire de Photonique et de Nanostructures, CNRS, Route de Nozay, Marcoussis (France); Kefelian, C. [Univ Lyon, Universite Claude Bernard Lyon 1, CNRS/IN2P3, Institut de Physique Nucleaire de Lyon, Lyon (France); Karlsruher Institut fuer Technologie, Institut fuer Experimentelle Kernphysik, Karlsruhe (Germany); Kleifges, M.; Tcherniakhovski, D.; Weber, M. [Karlsruher Institut fuer Technologie, Institut fuer Prozessdatenverarbeitung und Elektronik, Karlsruhe (Germany); Kraus, H. [University of Oxford, Department of Physics, Oxford (United Kingdom); Kudryavtsev, V.A. [University of Sheffield, Department of Physics and Astronomy, Sheffield (United Kingdom); Pari, P. [CEA Saclay, DSM/IRAMIS, Gif-sur-Yvette (France); Piro, M.C. [CSNSM, Univ. Paris-Sud, CNRS/IN2P3, Universite Paris-Saclay, Orsay (France); Rensselaer Polytechnic Institute, Troy, NY (United States); Rozov, S.; Yakushev, E. [JINR, Laboratory of Nuclear Problems, Dubna, Moscow Region (Russian Federation); Schmidt, B. [Karlsruher Institut fuer Technologie, Institut fuer Kernphysik, Karlsruhe (Germany); Lawrence Berkeley National Laboratory, Berkeley, CA (United States)

    2016-10-15

    We report on a dark matter search for a Weakly Interacting Massive Particle (WIMP) in the mass range m{sub χ} element of [4, 30] GeV/c{sup 2} with the EDELWEISS-III experiment. A 2D profile likelihood analysis is performed on data from eight selected detectors with the lowest energy thresholds leading to a combined fiducial exposure of 496 kg-days. External backgrounds from γ- and β-radiation, recoils from {sup 206}Pb and neutrons as well as detector intrinsic backgrounds were modelled from data outside the region of interest and constrained in the analysis. The basic data selection and most of the background models are the same as those used in a previously published analysis based on boosted decision trees (BDT) [1]. For the likelihood approach applied in the analysis presented here, a larger signal efficiency and a subtraction of the expected background lead to a higher sensitivity, especially for the lowest WIMP masses probed. No statistically significant signal was found and upper limits on the spin-independent WIMP-nucleon scattering cross section can be set with a hypothesis test based on the profile likelihood test statistics. The 90 % C.L. exclusion limit set for WIMPs with m{sub χ} = 4 GeV/c{sup 2} is 1.6 x 10{sup -39} cm{sup 2}, which is an improvement of a factor of seven with respect to the BDT-based analysis. For WIMP masses above 15 GeV/c{sup 2} the exclusion limits found with both analyses are in good agreement. (orig.)

  2. Extending the Sensitivity to the Detection of WIMP Dark Matter with an Improved Understanding of the Limiting Neutron Backgrounds

    Energy Technology Data Exchange (ETDEWEB)

    Kamat, Sharmila [Case Western Reserve Univ., Cleveland, OH (United States). Dept. of Physics

    2005-01-01

    The Cryogenic Dark Matter Search (CDMS) uses position-sensitive Germanium and Silicon crystals in the direct detection of Weakly Interacting Massive Particles (WIMPs) believed to constitute most of the dark matter in the Universe. WIMP interactions with matter being rare, identifying and eliminating known backgrounds is critical for detection. Event-by-event discrimination by the detectors rejects the predominant gamma and beta backgrounds while Monte Carlo simulations help estimate, and subtract, the contribution from the neutrons. This thesis describes the effort to understand neutron backgrounds as seen in the two stages of the CDMS search for WIMPs. The first stage of the experiment was at a shallow site at the Stanford Underground Facility where the limiting background came from high-energy neutrons produced by cosmic-ray muon interactions in the rock surrounding the cavern. Simulations of this background helped inform the analysis of data from an experimental run at this site and served as input for the background reduction techniques necessary to set new exclusion limits on the WIMP-nucleon cross-section, excluding new parameter space for WIMPs of masses 8-20 GeV/c2. This thesis considers the simulation methods used as well as how various event populations in the data served as checks on the simulations to allow them to be used in the interpretation of the WIMP-search data. The studies also confirmed the presence of a limiting neutron background at the shallow site, necessitating the move to the 713-meter deep Soudan Underground Facility. Similar computer-based studies helped quantify the neutron background seen at the deeper site and informed the analysis of the data emerging from the first physics run of the experiment at Soudan. In conjunction with the WIMP-search and calibration data, the simulations confirmed that increased depth considerably reduced the neutron backgrounds seen, greatly improving the sensitivity to WIMP detection. The data

  3. Working Group Report: WIMP Dark Matter Direct Detection

    Energy Technology Data Exchange (ETDEWEB)

    Cushman, P.; Galbiati, C.; McKinsey, D. N.; Robertson, H.; Tait, T. M.P.

    2013-10-30

    As part of the Snowmass process, the Cosmic Frontier WIMP Direct Detection subgroup (CF1) has drawn on input from the Cosmic Frontier and the broader Particle Physics community to produce this document. The charge to CF1 was (a) to summarize the current status and projected sensitivity of WIMP direct detection experiments worldwide, (b) motivate WIMP dark matter searches over a broad parameter space by examining a spectrum of WIMP models, (c) establish a community consensus on the type of experimental program required to explore that parameter space, and (d) identify the common infrastructure required to practically meet those goals.

  4. WIMP Theory Review

    OpenAIRE

    Queiroz, Farinaldo S.

    2017-01-01

    The complementarity of direct, indirect and collider searches for dark matter has improved our understanding concerning the properties of the dark matter particle. In this short review, we present a step toward the fundamental nature of dark matter with direct detection experiments only, go through some of the potential dark matter signals in gamma-rays, x-rays, and anti-matter, and lastly discuss the prospects of WIMPs in the next decade.

  5. The Fraternal WIMP Miracle

    CERN Document Server

    Craig, Nathaniel

    2015-01-01

    We identify and analyze thermal dark matter candidates in the fraternal twin Higgs model and its generalizations. The relic abundance of fraternal twin dark matter is set by twin weak interactions, with a scale tightly tied to the weak scale of the Standard Model by naturalness considerations. As such, the dark matter candidates benefit from a "fraternal WIMP miracle," reproducing the observed dark matter abundance for dark matter masses between 50 and 150 GeV. However, the couplings dominantly responsible for dark matter annihilation do not lead to interactions with the visible sector. The direct detection rate is instead set via fermionic Higgs portal interactions, which are likewise constrained by naturalness considerations but parametrically weaker than those leading to dark matter annihilation. The predicted direct detection cross section is close to current LUX bounds and presents an opportunity for the next generation of direct detection experiments.

  6. On the Trail of WIMPs

    Indian Academy of Sciences (India)

    The fundamental particle nature of the Dark Matter (DM)that constitutes more than 80% of the gravitating matter inthe Universe is unknown. There is no suitable candidate forthis DM in the Standard Model (SM) of particle physics. Theso-called Weakly Interacting Massive Particles (WIMPs), predictedin many scenarios of ...

  7. On the Trail of WIMPs

    Indian Academy of Sciences (India)

    The fundamental particle nature of the Dark Matter (DM) that constitutes more than 80% of the gravitating matter in the Universe is unknown. There is no suitable candidate for this DM in the Standard Model (SM) of particle physics. The so-called Weakly Interacting Massive Particles (WIMPs), pre- dicted in many scenarios of ...

  8. First spin-dependent WIMP-nucleon cross section limits from the LUX experiment

    CERN Document Server

    Akerib, D S; Bai, X; Bailey, A J; Balajthy, J; Beltrame, P; Bernard, E P; Bernstein, A; Biesiadzinski, T P; Boulton, E M; Bradley, A; Bramante, R; Cahn, S B; Carmona-Benitez, M C; Chan, C; Chapman, J J; Chiller, A A; Chiller, C; Currie, A; Cutter, J E; Davison, T J R; de Viveiros, L; Dobi, A; Dobson, J E Y; Druszkiewicz, E; Edwards, B N; Faham, C H; Fiorucci, S; Gaitskell, R J; Gehman, V M; Ghag, C; Gibson, K R; Gilchriese, M G D; Hall, C R; Hanhardt, M; Haselschwardt, S J; Hertel, S A; Hogan, D P; Horn, M; Huang, D Q; Ignarra, C M; Ihm, M; Jacobsen, R G; Ji, W; Kazkaz, K; Khaitan, D; Knoche, R; Larsen, N A; Lee, C; Lenardo, B G; Lesko, K T; Lindote, A; Lopes, M I; Malling, D C; Manalaysay, A; Mannino, R L; Marzioni, M F; McKinsey, D N; Mei, D M; Mock, J; Moongweluwan, M; Morad, J A; Murphy, A St J; Nehrkorn, C; Nelson, H N; Neves, F; O'Sullivan, K; Oliver-Mallory, K C; Ott, R A; Palladino, K J; Pangilinan, M; Pease, E K; Phelps, P; Reichhart, L; Rhyne, C; Shaw, S; Shutt, T A; Silva, C; Solovov, V N; Sorensen, P; Stephenson, S; Sumner, T J; Szydagis, M; Taylor, D J; Taylor, W; Tennyson, B P; Terman, P A; Tiedt, D R; To, W H; Tripathi, M; Tvrznikova, L; Uvarov, S; Verbus, J R; Webb, R C; White, J T; Whitis, T J; Witherell, M S; Wolfs, F L H; Yazdani, K; Young, S K; Zhang, C

    2016-01-01

    We present the first experimental constraints on the spin-dependent WIMP-nucleon elastic cross sections from LUX data acquired in 2013. LUX is a dual-phase xenon time projection chamber operating at the Sanford Underground Research Facility (Lead, South Dakota), which is designed to observe the recoil signature of galactic WIMPs scattering from xenon nuclei. A profile likelihood ratio analysis of $1.4~\\times~10^{4}~\\text{kg}\\cdot~\\text{days}$ of fiducial exposure allows 90% CL upper limits to be set on the WIMP-neutron (WIMP-proton) cross section of $\\sigma_n~=~9.4~\\times~10^{-41}~\\text{cm}^2$ ($\\sigma_p~=~2.9~\\times~10^{-39}~\\text{cm}^2$) at 33~GeV/c$^2$. The spin-dependent WIMP-neutron limit is the most sensitive constraint to date for this channel.

  9. Direct WIMP searches with XENON100 and XENON1T

    Directory of Open Access Journals (Sweden)

    Davide Ferella Alfredo

    2015-01-01

    Full Text Available The XENON100 experiment is the second phase of the XENON direct Dark Matter search program. It consists of an ultra-low background double phase (liquid-gas xenon filled time projection chamber with a total mass of 161 kg (62 in the target region and 99 in the active shield, installed at the Laboratori Nazionali del Gran Sasso (LNGS. Here the results from the 224.6 live days of data taken between March 2011 and April 2012 are reported. The experiment set one of the most stringent limits on the WIMP-nucleon spin-independent cross section to date (2 × 10−45 cm2 for a 55 Gev/c2 WIMP mass at 90 % confidence level and the most stringent on the spin-dependent WIMP-neutron interaction (3.5 × 10−40 for a 45 GeV/c2 WIMP mass. With the same dataset, XENON100 excludes also solar axion coupling to electrons at gAe > 7.7 × 10−12 for a mass of mAxion 1 × 10−12 at a mass range of mAxion = 5−10 keV/c2 (both 90 % C.L.. Moreover an absolute spectral comparison between simulated and measured nuclear recoil distributions of light and charge signals from a 241AmBe source demonstrates a high level of detector and systematics understanding. Finally, the third generation of the XENON experiments, XENON1T, is the first tonne scale direct WIMP search experiment currently under construction. The commissioning phase of XENON1T is expected to start in early 2015 followed, a few months after, by the first science run. The experiment will reach sensitivities on the WIMP-nucleon spin-independent cross section down to 2 ×10−47 cm2 after two years of data taking.

  10. Photon spectra from WIMP annihilation

    OpenAIRE

    Ruiz Cembranos, José Alberto; Cruz Dombriz, Álvaro de la; Dobado González, Antonio; Lineros, R. A.; López Maroto, Antonio

    2010-01-01

    If the present dark matter in the Universe annihilates into standard model particles, it must contribute to the fluxes of cosmic rays that are detected on the Earth and, in particular, to the observed gamma-ray fluxes. The magnitude of such a contribution depends on the particular dark matter candidate, but certain features of the produced photon spectra may be analyzed in a rather model-independent fashion. In this work we provide the complete photon spectra coming from WIMP annihilation int...

  11. WIMP detection and slow ion dynamics in carbon nanotube arrays

    CERN Document Server

    Cavoto, G.; Cocina, F.; Ferretti, J.; Polosa, A.D.

    2016-06-24

    Large arrays of aligned carbon nanotubes (CNTs), open at one end, could be used as target material for the directional detection of weakly interacting dark matter particles (WIMPs). As a result of a WIMP elastic scattering on a CNT, a carbon ion might be injected in the body of the array and propagate through multiple collisions within the lattice. The ion may eventually emerge from the surface with open end CNTs, provided that its longitudinal momentum is large enough to compensate energy losses and its transverse momentum approaches the channeling conditions in a single CNT. Therefore, the angle formed between the WIMP wind apparent orientation and the direction of parallel carbon nanotube axes must be properly chosen. We focus on very low ion recoil kinetic energies, related to low mass WIMPs (~ 10 GeV) where most of the existing experiments have low sensitivity. Relying on some exact results on two-dimensional lattices of circular obstacles, we study the low energy ion motion in the transverse plane with ...

  12. WIMP detection and slow ion dynamics in carbon nanotube arrays.

    Science.gov (United States)

    Cavoto, G; Cirillo, E N M; Cocina, F; Ferretti, J; Polosa, A D

    2016-01-01

    Large arrays of aligned carbon nanotubes (CNTs), open at one end, could be used as target material for the directional detection of weakly interacting dark matter particles (WIMPs). As a result of a WIMP elastic scattering on a CNT, a carbon ion might be injected in the body of the array and propagate through multiple collisions within the lattice. The ion may eventually emerge from the surface with open end CNTs, provided that its longitudinal momentum is large enough to compensate energy losses and its transverse momentum approaches the channeling conditions in a single CNT. Therefore, the angle formed between the WIMP wind apparent orientation and the direction of parallel carbon nanotube axes must be properly chosen. We focus on very low ion recoil kinetic energies, related to low mass WIMPs ([Formula: see text] GeV) where most of the existing experiments have low sensitivity. Relying on some exact results on two-dimensional lattices of circular obstacles, we study the low energy ion motion in the transverse plane with respect to CNT directions. New constraints are obtained on how to devise the CNT arrays to maximize the target channeling efficiency.

  13. WIMP detection and slow ion dynamics in carbon nanotube arrays

    Energy Technology Data Exchange (ETDEWEB)

    Cavoto, G. [INFN Sezione di Roma, Rome (Italy); Cirillo, E.N.M. [Sapienza Universita di Roma, Dipartimento SBAI, Rome (Italy); Cocina, F. [Sapienza Universita di Roma, Dipartimento di Fisica, Rome (Italy); Ferretti, J. [Sapienza Universita di Roma, Dipartimento di Fisica (Italy); INFN Sezione di Roma, Rome (Italy); Polosa, A.D. [Sapienza Universita di Roma, Dipartimento di Fisica (Italy); CERN, Theory Division, Geneva (Switzerland); INFN Sezione di Roma, Rome (Italy)

    2016-06-15

    Large arrays of aligned carbon nanotubes (CNTs), open at one end, could be used as target material for the directional detection of weakly interacting dark matter particles (WIMPs). As a result of a WIMP elastic scattering on a CNT, a carbon ion might be injected in the body of the array and propagate through multiple collisions within the lattice. The ion may eventually emerge from the surface with open end CNTs, provided that its longitudinal momentum is large enough to compensate energy losses and its transverse momentum approaches the channeling conditions in a single CNT. Therefore, the angle formed between the WIMP wind apparent orientation and the direction of parallel carbon nanotube axes must be properly chosen. We focus on very low ion recoil kinetic energies, related to low mass WIMPs (∼ 11 GeV) where most of the existing experiments have low sensitivity. Relying on some exact results on two-dimensional lattices of circular obstacles, we study the low energy ion motion in the transverse plane with respect to CNT directions. New constraints are obtained on how to devise the CNT arrays to maximize the target channeling efficiency. (orig.)

  14. A DEAP & CLEAN Program for WIMP Dark Matter

    Science.gov (United States)

    Hime, Andrew

    2007-04-01

    Weakly Interacting Massive Particles (WIMPs) provide a compelling explanation for dark matter that could be directly detected as they recoil in massive and ultra-pure detector targets operating deep underground. Thus far, the direct detection of WIMPs has eluded the most sensitive of experiments and it is desirable to achieve sensitivities some three orders of magnitude beyond the state-of-the-art. This will require development of novel detector technologies exploiting target masses of order a ton and with unprecedented control of radioactive background in order to achieve the desired sensitivity of a single event per ton per year. We have realized a conceptually simple and scalable detector technology using the unique properties of scintillation light in liquid argon and liquid neon. Ultimately, we envision a very massive detector of liquid neon that could serve simultaneously as a detector of WIMP dark matter and low-energy (pp-fusion) solar neutrinos. A DEAP & CLEAN program will be described that makes effective use of liquid argon and liquid neon, interchangeably, in a single detector.

  15. A search for WIMPs with the CoGeNT public dataset

    Science.gov (United States)

    Bellis, Matthew; Kelso, Chris; Collar, Juan

    2016-03-01

    Since December 2009, the CoGeNT experiment has recorded interactions in the detector with the goal of either detecting dark matter or setting stringent limits on the mass and cross-section of these particles, assuming that dark matter is a form of WIMP (Weakly Interacting Massive Particle). The collaboration has made public this dataset to the broader community and this analysis is based on that dataset. We perform an unbinned, maximum likelihood fit to the data, accounting for known backgrounds and systematic effects. {articlar care is paid to detector effects which can mimic the energy distribution of WIMP interactions. We model the WIMP signal, parametrized by energy deposition and time of year, mass, cross-section, and choice of local WIMP velocity distribution. Monte Carlo studies are performed to verify the stability and sensitivity of the fits and all code used in the analysis is made publicly available. The current status of this analysis will be presented.

  16. NEWSdm: Nuclear Emulsions for WIMP Search with directional measurement

    Science.gov (United States)

    Di Crescenzo, A.

    2017-12-01

    Direct Dark Matter searches are nowadays one of the most exciting research topics. Several experimental efforts are concentrated on the development, construction, and operation of detectors looking for the scattering of target nuclei with Weakly Interactive Massive Particles (WIMPs). The measurement of the direction of WIMP-induced nuclear recoils is a challenging strategy to extend dark matter searches beyond the neutrino floor and provide an unambiguous signature of the detection of Galactic dark matter. Current directional experiments are based on the use of gas TPC whose sensitivity is strongly limited by the small achievable detector mass. We present an innovative directional experiment based on the use of a solid target made by newly developed nuclear emulsions and read-out systems reaching a position resolution of the order of 10 nm.

  17. NEWSdm: Nuclear Emulsions for WIMP Search with directional measurement

    Directory of Open Access Journals (Sweden)

    Di Crescenzo A.

    2017-01-01

    Full Text Available Direct Dark Matter searches are nowadays one of the most exciting research topics. Several experimental efforts are concentrated on the development, construction, and operation of detectors looking for the scattering of target nuclei with Weakly Interactive Massive Particles (WIMPs. The measurement of the direction of WIMP-induced nuclear recoils is a challenging strategy to extend dark matter searches beyond the neutrino floor and provide an unambiguous signature of the detection of Galactic dark matter. Current directional experiments are based on the use of gas TPC whose sensitivity is strongly limited by the small achievable detector mass. We present an innovative directional experiment based on the use of a solid target made by newly developed nuclear emulsions and read-out systems reaching a position resolution of the order of 10 nm.

  18. WIMPs at the Galactic Center

    CERN Document Server

    Agrawal, Prateek; Fox, Patrick J.; Harnik, Roni

    2015-01-01

    Simple models of weakly interacting massive particles (WIMPs) predict dark matter annihilations into pairs of electroweak gauge bosons, Higgses or tops, which through their subsequent cascade decays produce a spectrum of gamma rays. Intriguingly, an excess in gamma rays coming from near the Galactic center has been consistently observed in Fermi data. A recent analysis by the Fermi collaboration confirms these earlier results. Taking into account the systematic uncertainties in the modelling of the gamma ray backgrounds, we show for the first time that this excess can be well fit by these final states. In particular, for annihilations to $(WW, ZZ, hh, tt)$, dark matter with mass between threshold and approximately (165, 190, 280, 310) GeV gives an acceptable fit. The fit range for $b\\bar{b}$ is also enlarged to 35 GeV$\\lesssim m_\\chi \\lesssim$ 165 GeV. These are to be compared to previous fits that concluded only much lighter dark matter annihilating into $b$, $\\tau$, and light quark final states could descri...

  19. Halo-independent determination of the unmodulated WIMP signal in DAMA: the isotropic case

    Science.gov (United States)

    Gondolo, Paolo; Scopel, Stefano

    2017-09-01

    We present a halo-independent determination of the unmodulated signal corresponding to the DAMA modulation if interpreted as due to dark matter weakly interacting massive particles (WIMPs). First we show how a modulated signal gives information on the WIMP velocity distribution function in the Galactic rest frame from which the unmodulated signal descends. Then we describe a mathematically-sound profile likelihood analysis in which the likelihood is profiled over a continuum of nuisance parameters (namely, the WIMP velocity distribution). As a first application of the method, which is very general and valid for any class of velocity distributions, we restrict the analysis to velocity distributions that are isotropic in the Galactic frame. In this way we obtain halo-independent maximum-likelihood estimates and confidence intervals for the DAMA unmodulated signal. We find that the estimated unmodulated signal is in line with expectations for a WIMP-induced modulation and is compatible with the DAMA background+signal rate. Specifically, for the isotropic case we find that the modulated amplitude ranges between a few percent and about 25% of the unmodulated amplitude, depending on the WIMP mass.

  20. Improved WIMP-search reach of the CDMS II germanium data

    Energy Technology Data Exchange (ETDEWEB)

    Agnese, R.; Anderson, A. J.; Asai, M.; Balakishiyeva, D.; Barker, D.; Basu Thakur, R.; Bauer, D. A.; Billard, J.; Borgland, A.; Bowles, M. A.; Brandt, D.; Brink, P. L.; Bunker, R.; Cabrera, B.; Caldwell, D. O.; Calkins, R.; Cerdeno, D. G.; Chagani, H.; Chen, Y.; Cooley, J.; Cornell, B.; Crewdson, C. H.; Cushman, Priscilla B.; Daal, M.; Di Stefano, P. C.; Doughty, T.; Esteban, L.; Fallows, S.; Figueroa-Feliciano, E.; Godfrey, G. L.; Golwala, S. R.; Hall, Jeter C.; Harris, H. R.; Hertel, S. A.; Hofer, T.; Holmgren, D.; Hsu, L.; Huber, M. E.; Jardin, D. M.; Jastram, A.; Kamaev, O.; Kara, B.; Kelsey, M. H.; Kennedy, A.; Kiveni, M.; Koch, K.; Leder, A.; Loer, B.; Lopez Asamar, E.; Lukens, P.; Mahapatra, R.; Mandic, V.; McCarthy, K. A.; Mirabolfathi, N.; Moffatt, R. A.; Oser, S. M.; Page, K.; Page, W. A.; Partridge, R.; Pepin, M.; Phipps, A.; Prasad, K.; Pyle, M.; Qiu, H.; Rau, W.; Redl, P.; Reisetter, A.; Ricci, Y.; Rogers, H. E.; Saab, T.; Sadoulet, B.; Sander, J.; Schneck, K.; Schnee, R. W.; Scorza, S.; Serfass, B.; Shank, B.; Speller, D.; Toback, D.; Upadhyayula, S.; Villano, A. N.; Welliver, B.; Wilson, J. S.; Wright, D. H.; Yang, X.; Yellin, S.; Yen, J. J.; Young, B. A.; Zhang, J.

    2015-10-31

    CDMS II data from the five-tower runs at the Soudan Underground Laboratory were reprocessed with an improved charge-pulse fitting algorithm. Two new analysis techniques to reject surface-event backgrounds were applied to the 612 kg days germanium-detector weakly interacting massive particle (WIMP)-search exposure. An extended analysis was also completed by decreasing the 10 keV analysis threshold to ~5 keV, to increase sensitivity near a WIMP mass of 8 GeV/c2. After unblinding, there were zero candidate events above a deposited energy of 10 keV and six events in the lower-threshold analysis. This yielded minimum WIMP-nucleon spin-independent scattering cross-section limits of 1.8×10-44 and 1.18×10-41 at 90% confidence for 60 and 8.6 GeV/c2 WIMPs, respectively. This improves the previous CDMS II result by a factor of 2.4 (2.7) for 60 (8.6) GeV/c2 WIMPs.

  1. Boffins go underground searching for Wimps

    CERN Multimedia

    2003-01-01

    In a bid to identify the prime suspect for Dark Matter known as Weakly Interacting Massive Particles, or Wimps, British scientists have installed detectors 1100m down a salt mine at Boulby on the North Yorkshire moors (1/2 page).

  2. Power Corrections to the Universal Heavy WIMP-Nucleon Cross Section

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Chien-Yi; Hill, Richard J.; Solon, Mikhail P.; Wijangco, Alexander M.

    2018-01-25

    WIMP-nucleon scattering is analyzed at order $1/M$ in Heavy WIMP Effective Theory. The $1/M$ power corrections, where $M\\gg m_W$ is the WIMP mass, distinguish between different underlying UV models with the same universal limit and their impact on direct detection rates can be enhanced relative to naive expectations due to generic amplitude-level cancellations at leading order. The necessary one- and two-loop matching calculations onto the low-energy effective theory for WIMP interactions with Standard Model quarks and gluons are performed for the case of an electroweak SU(2) triplet WIMP, considering both the cases of elementary fermions and composite scalars. The low-velocity WIMP-nucleon scattering cross section is evaluated and compared with current experimental limits and projected future sensitivities. Our results provide the most robust prediction for electroweak triplet Majorana fermion dark matter direct detection rates; for this case, a cancellation between two sources of power corrections yields a small total $1/M$ correction, and a total cross section close to the universal limit for $M \\gtrsim {\\rm few} \\times 100\\,{\\rm GeV}$. For the SU(2) composite scalar, the $1/M$ corrections introduce dependence on underlying strong dynamics. Using a leading chiral logarithm evaluation, the total $1/M$ correction has a larger magnitude and uncertainty than in the fermionic case, with a sign that further suppresses the total cross section. These examples provide definite targets for future direct detection experiments and motivate large scale detectors capable of probing to the neutrino floor in the TeV mass regime.

  3. A map of the non-thermal WIMP

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Hyungjin, E-mail: hjkim06@kaist.ac.kr [Department of Physics, KAIST, Daejeon 34141 (Korea, Republic of); Center for Theoretical Physics of the Universe, Institute for Basic Science (IBS), Daejeon 34051 (Korea, Republic of); Hong, Jeong-Pyong, E-mail: hjp0731@icrr.u-tokyo.ac.jp [Institute for Cosmic Ray Research, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8582 (Japan); Kavli IPMU (WPI), UTIAS, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8583 (Japan); Shin, Chang Sub, E-mail: changsub.shin@apctp.org [Asia Pacific Center for Theoretical Physics, Pohang 37673 (Korea, Republic of); Department of Physics, Postech, Pohang 37673 (Korea, Republic of)

    2017-05-10

    We study the effect of the elastic scattering on the non-thermal WIMP, which is produced by direct decay of heavy particles at the end of reheating. The non-thermal WIMP becomes important when the reheating temperature is well below the freeze-out temperature. Usually, two limiting cases have been considered. One is that the produced high energetic dark matter particles are quickly thermalized due to the elastic scattering with background radiations. The corresponding relic abundance is determined by the thermally averaged annihilation cross-section at the reheating temperature. The other one is that the initial abundance is too small for the dark matter to annihilate so that the final relic is determined by the initial amount itself. We study the regions between these two limits, and show that the relic density depends not only on the annihilation rate, but also on the elastic scattering rate. Especially, the relic abundance of the p-wave annihilating dark matter crucially relies on the elastic scattering rate because the annihilation cross-section is sensitive to the dark matter velocity. We categorize the parameter space into several regions where each region has distinctive mechanism for determining the relic abundance of the dark matter at the present Universe. The consequence on the (in)direct detection is also studied.

  4. WIMP Dark Matter and the First Stars

    Science.gov (United States)

    Iocco, Fabio

    2010-11-01

    If weakly interacting massive particles (WIMPs) constitute the bulk of dark matter (DM), energy from the self-annihilation of these particles can affect Population III (Pop III) star formation via two mechanisms. Before the protostar forms, energy from DM annihilations can couple to primordial gas chemistry and slightly alter the properties of the cloud-without, however, inducing dramatic changes in the final mass of the star. Later, scattering between WIMPs and baryons within the protostar can in principle congregate enough DM for annihilations, rather than nuclear reactions, to support the star against gravity. In these proceedings I briefly summarize the state of the art of the field, as well the prospects for observing such stars.

  5. Louisiana ESI: ESI (Environmental Sensitivity Index Shoreline Types - Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines representing the shoreline and coastal habitats of Louisiana classified according to the Environmental Sensitivity Index (ESI)...

  6. Impact of a 'heavy' SIMPLE on the search for WIMP dark matter

    Energy Technology Data Exchange (ETDEWEB)

    Giuliani, F [Centro de Fisica Nuclear, Universidade de Lisboa, 1649-003 Lisbon (Portugal); Morlat, T [Centro de Fisica Nuclear, Universidade de Lisboa, 1649-003 Lisbon (Portugal); Girard, Ta [Centro de Fisica Nuclear, Universidade de Lisboa, 1649-003 Lisbon (Portugal); Costa, M Felizardo da [Centro de Fisica Nuclear, Universidade de Lisboa, 1649-003 Lisbon (Portugal); Marques, Jg [Instituto Tecnologico e Nuclear, Estrada Nacional 10, 2686-953 Sacavem (Portugal); Ramos, Ar [Instituto Tecnologico e Nuclear, Estrada Nacional 10, 2686-953 Sacavem (Portugal)

    2006-05-15

    We examine the potential impact of a WIMP search based on CF3I-loaded SDDs, projected on the basis of the experience and results of the SIMPLE dark matter search. We find such a 'heavy' SIMPLE experiment to have spin-independent sensitivity comparable to that of leading searches like ZEPLIN-I and CDMS, while preserving the spin-dependent sensitivity of fluorine-based experiments.

  7. Planck-scale effects on WIMP dark matter

    Directory of Open Access Journals (Sweden)

    Sofiane M Boucenna

    2014-01-01

    Full Text Available There exists a widely known conjecture that gravitational effects violate global symmetries. We study the effect of global-symmetry violating higher-dimension operators induced by Planck-scale physics on the properties of WIMP dark matter. Using an effective description, we show that the lifetime of the WIMP dark matter candidate can satisfy cosmological bounds under reasonable assumptions regarding the strength of the dimension-five operators. On the other hand, the indirect WIMP dark matter detection signal is significantly enhanced due to new decay channels.

  8. In-line phase sensitive amplifier based on PPLN waveguides.

    Science.gov (United States)

    Umeki, Takeshi; Asobe, Masaki; Takenouchi, Hirokazu

    2013-05-20

    We demonstrate a χ(2)-based in-line PSA with a carrier-recovery and phase-locking system for a phase shift keying (PSK) signal. By doubling the signal phase using a wavelength conversion technique, the carrier was recovered from a PSK signal. The carrier phase was synchronized to a local oscillator using optical injection locking. Phase sensitive amplification with a wide phase sensitive dynamic range of 20 dB was achieved using degenerate parametric amplification in a periodically poled LiNbO(3) (PPLN) waveguide. The phase regeneration effect was examined for a degraded signal by means of constellation analyses and bit-error rate measurements. The in-line PSA also operated successfully as a repeater amplifier in a 160 km fiber link without a power penalty. Finally, we demonstrate the regeneration of non-linear impairments induced by fiber non-linearity.

  9. The phenomenology of superWIMP dark matter scenariow with long-lived sleptons

    Energy Technology Data Exchange (ETDEWEB)

    Heisig, Jan

    2013-08-15

    We study the phenomenology of a supersymmetric scenario where the next-to-lightest superparticle (NLSP) is the charged slepton and is long-lived due to a lightest superparticle (LSP) which is a super weakly interacting massive particle (superWIMP), like the gravitino. This has far-reaching consequences for the cosmological history of the universe on the one hand and for the signatures at colliders on the other hand. We do not assume any high-scale model for the mediation of SUSY breaking to the MSSM but work along the lines of simplified models and the phenomenological MSSM (pMSSM). In a first part, we investigate the LHC sensitivity and its dependence on the superparticle spectrum with an emphasis on strong production and decay. We formulate appropriate simplified models that allow to conservatively approximate the signal efficiencies of arbitrary spectra from a small number of decisive parameters. We found that the application of simplified models is especially suitable in the considered scenario. Devising cuts that yield a large detection efficiency in the whole parameter space, we determine the discovery and exclusion potential of the LHC. We found that the prominent signature of long-lived sleptons allows to extract more robust constraints on the parameter space than for the widely studied case of a neutralino LSP scenario. In addition, we study the implications of the recent LHC results on the cosmological validity of a superWIMP Dark Matter scenario with a long-lived stau NLSP. Therefore, we work in a pMSSM framework and perform a Monte Carlo scan over the pMSSM parameter space highlighting the implications of a Higgs around 125 GeV and the nullsearches for heavy stable charged particles at the 7 and 8TeV LHC. Further, we consider bounds from MSSM Higgs searches, from flavor and precision observables as well as from the theoretical requirement of vacuum stability. In particular we work out the impact on the allowed range for the stau yield after freeze

  10. DNA methylation and sensitivity to antimetabolites in cancer cell lines.

    Science.gov (United States)

    Sasaki, Shin; Kobunai, Takashi; Kitayama, Joji; Nagawa, Hirokazu

    2008-02-01

    The prediction of the cellular direction of metabolic pathways toward either DNA synthesis or DNA methylation is crucial for determining the susceptibility of cancers to anti-metabolites such as fluorouracil (5-FU). We genotyped the methylenetetrahydrofolate reductase (MTHFR) gene in NCI-60 cancer cell lines, and identified the methylation status of 24 tumor suppressor genes using methylation-specific multiplex ligation-dependent probe amplification. The susceptibility of the cancer cell lines to seven antimetabolites was then determined. Cells homozygous for CC at MTHFR-A1298C were significantly more sensitive to cyclocytidine, cytarabine (AraC) and floxuridine than those with AA or AC (p=0.0215, p=0.0166, and p=0.0323, respectively), and carried more methylated tumor suppressor genes (p=0.0313). Among the 12 tumor suppressor genes which were methylated in >25% of cancer cell lines, the methylation status of TIMP3, APC and IGSF4 significantly correlated with sensitivity to pyrimidine synthesis inhibitors. In particular, cells with methylated TIMP3 had reduced mRNA levels and were significantly more sensitive to aphidicolin-glycinate, AraC and 5-FU than cells with unmethylated TIMP3. We speculate that MTHFR-A1298C homozygous CC might direct the methylation rather than the synthesis of DNA, and result in the methylation of several tumor suppressor genes such as TIMP3. These genes could be useful biological markers for predicting the efficacy of antimetabolites.

  11. Caffeine markedly sensitizes human mesothelioma cell lines to pemetrexed

    Science.gov (United States)

    Min, Sang Hee; Goldman, I. David; Zhao, Rongbao

    2013-01-01

    Pemetrexed is a new generation antifolate approved for the treatment of mesothelioma and non-small cell lung cancer. Caffeine is known to augment radiation or chemotherapeutic drug-induced cell killing. The current study addresses the impact of caffeine on the activity of pemetrexed in mesothelioma cell lines. Caffeine enhanced pemetrexed activity in all four mesothelioma cell lines tested (H2052, H2373, H28 and MSTO-211H). Caffeine sensitized H2052 cells in a dose- and schedule-dependent manner, and was associated with a markedly decreased clonogenic survival. Caffeine sensitization occurred only in cells subjected to pulse, but not continuous, exposure to pemetrexed. Similar pemetrexed sensitization was also observed with the clinically better tolerated caffeine analog, theobromine. Pemetrexed sensitization by caffeine was associated with an increase in pemetrexed-induced phosphorylation of ataxia-telangiectasia-mutated (ATM) and Chk1. These data indicate that caffeine and its analog, theobromine, may be a useful approach to enhance pemetrexed-based chemotherapy. PMID:17594092

  12. Science plumbs new depths in hunt for Wimps

    CERN Multimedia

    Benfield, C

    2003-01-01

    Lord Sainsbury has officially opened a laboratory in the salt mine at Boulby near Whitby. The lab is searching for WIMPs and was recently awarded 3.1 millions pounds by PPARC to upgrade the facility (1 page).

  13. Boron-lined proportional counters with improved neutron sensitivity

    CERN Document Server

    Dighe, P M; Prasad, K R; Kataria, S K; Athavale, S N; Pappachan, A L; Grover, A K

    2003-01-01

    Boron-lined proportional counters with higher neutron sensitivity have been developed by introducing baffle structures within the sensitive volume. the results are compared to devices developed with multiple cathode assemblies in a single enclosure. in either case, the increase in the boron-coated surface area results in higher neutron sensitivity. one of these counters has 51 annular baffles coated with natural boron with 10 mm hole for the anode wire to pass through. filled with p-10 gas at 20 cm hg, it has an overall diameter of 30 and 300 mm length. multiple dip coating method was employed for better uniformity in boron thickness. the neutron sensitivity of this counter is 1.6 cps/nv, which is 2.5 times that of a counter with standard electrode geometry. another counter was developed with three cathode assemblies (30 mm IDx300 mm) coated with 92% sup 1 sup 0 B while the third has seven assemblies coated with natural boron (16 mm IDx750 mm length). the neutron sensitivity is 10 and 5.5 cps/nv, respectively...

  14. Status of TeO2 Detectors for WIMPS Searches

    Science.gov (United States)

    Alessandrello, A.; Brofferio, C.; Cremonesi, O.; Fiorini, E.; Nucciotii, A.; Pavan, M.; Pessina, G.; Pirro, S.; Previtali, E.; Sisti, M.; Vanzini, M.; Zanotti, L.; Bucci, C.; Pobes, C.; Giuliani, A.

    Thanks to very high energy resolutions , low energy thresholds and very high sensitivity to nuclear recoils, cryogenic thermal detectors my play an important role in study and search dark matter particles of the universe. Milano group works with this type of detectors since many years and, at presents, operates the bigger cryogenic thermal detector with a total mass of 6.8 kg of TeO2 crystals segmented in 20 active channels. This detector is in operation since 1998 and has realized to search for double beta decay of 130Te. As a by product of this measurement also dark matter study will be conducted and, especially in the last year, a strong effort was made to further reduce threshold and spurious background in low energy region . The preliminary data on WIMPs searches with this detector is here reported . The improvement of this experiment will be the realization of the CUORICINO detector. Some preliminary tests on this new detector will be presented and briefly discussed.

  15. TREX-DM: a low-background Micromegas-based TPC for low-mass WIMP detection

    Energy Technology Data Exchange (ETDEWEB)

    Iguaz, F.J.; Garza, J.G.; Castel, J.F.; Cebrian, S.; Dafni, T.; Garcia, J.A.; Irastorza, I.G.; Lagraba, A.; Luzon, G.; Peiro, A. [Universidad de Zaragoza, Grupo de Fisica Nuclear y Astroparticulas, Zaragoza (Spain); Aznar, F. [Universidad de Zaragoza, Grupo de Fisica Nuclear y Astroparticulas, Zaragoza (Spain); Universidad de Zaragoza, Centro Universitario de la Defensa, Zaragoza (Spain)

    2016-10-15

    If Dark Matter is made of Weakly Interacting Massive Particles (WIMPs) with masses below ∝20 GeV, the corresponding nuclear recoils in mainstream WIMP experiments are of energies too close, or below, the experimental threshold. Gas Time Projection Chambers (TPCs) can be operated with a variety of target elements, offer good tracking capabilities and, on account of the amplification in gas, very low thresholds are achievable. Recent advances in electronics and in novel radiopure TPC readouts, especially micro-mesh gas structure (Micromegas), are improving the scalability and low-background prospects of gaseous TPCs. Here we present TREX-DM, a prototype to test the concept of a Micromegas-based TPC to search for low-mass WIMPs. The detector is designed to host an active mass of ∝0.300 kg of Ar at 10 bar, or alternatively ∝0.160 kg of Ne at 10 bar, with an energy threshold below 0.4 keVee, and is fully built with radiopure materials. We will describe the detector in detail, the results from the commissioning phase on surface, as well as a preliminary background model. The anticipated sensitivity of this technique may go beyond current experimental limits for WIMPs of masses of 2-8 GeV. (orig.)

  16. Limits on the spin-dependent WIMP-nucleon cross sections from the first science run of the ZEPLIN-III experiment.

    Science.gov (United States)

    Lebedenko, V N; Araújo, H M; Barnes, E J; Bewick, A; Cashmore, R; Chepel, V; Currie, A; Davidge, D; Dawson, J; Durkin, T; Edwards, B; Ghag, C; Horn, M; Howard, A S; Hughes, A J; Jones, W G; Joshi, M; Kalmus, G E; Kovalenko, A G; Lindote, A; Liubarsky, I; Lopes, M I; Lüscher, R; Lyons, K; Majewski, P; Murphy, A St J; Neves, F; da Cunha, J Pinto; Preece, R; Quenby, J J; Scovell, P R; Silva, C; Solovov, V N; Smith, N J T; Smith, P F; Stekhanov, V N; Sumner, T J; Thorne, C; Walker, R J

    2009-10-09

    We present new experimental constraints on the WIMP-nucleon spin-dependent elastic cross sections using data from the first science run of ZEPLIN-III, a two-phase xenon experiment searching for galactic dark matter weakly interacting massive particles based at the Boulby mine. Analysis of approximately 450 kg x days fiducial exposure allow us to place a 90%-confidence upper limit on the pure WIMP-neutron cross section of sigma(n)=1.9x10(-2) pb at 55 GeV/c(2) WIMP mass. Recent calculations of the nuclear spin structure based on the Bonn charge-dependent nucleon-nucleon potential were used for the odd-neutron isotopes 129Xe and 131Xe. These indicate that the sensitivity of xenon targets to the spin-dependent WIMP-proton interaction could be much lower than implied by previous calculations, whereas the WIMP-neutron sensitivity is impaired only by a factor of approximately 2.

  17. 6th Patras workshop on axions, WIMPs and WISPs (PATRAS 2010). Proceedings

    Energy Technology Data Exchange (ETDEWEB)

    Baudis, Laura; Schumann, Marc (eds.)

    2010-11-15

    The following topics were dealt with: Axions, WIMPs, WISPs, and neutrinos in the universe, laboratory experimental searching for WISPs, astrophysical experimental searching for WISPs, direct and indirect detection of WIMPs, new ideas and developments, visions, large laboratories. (HSI)

  18. Proceedings of the 4th Patras workshop on axions, WIMPs and WISPs

    Energy Technology Data Exchange (ETDEWEB)

    Lindner, Axel; Redondo, Javier; Ringwald, Andreas (eds.)

    2008-08-15

    The following topics were dealt with: Physical foundations for WIMPs, axions, and WISPS, signals from astrophysical sources, direct searches for dark matter WIMPs, new theoretical developments, new experimental approaches. (HSI)

  19. The moment of truth for WIMP dark matter.

    Science.gov (United States)

    Bertone, Gianfranco

    2010-11-18

    We know that dark matter constitutes 85 per cent of all the matter in the Universe, but we do not know of what it is made. Amongst the many dark matter candidates proposed, WIMPs (weakly interacting massive particles) occupy a special place, because they arise naturally from new theories that seek to extend the standard model of particle physics. With the advent of the Large Hadron Collider at CERN, and a new generation of astroparticle experiments, the moment of truth has come for WIMPs: either we will discover them in the next five to ten years, or we will witness their inevitable decline.

  20. Dark matter spin-dependent limits for WIMP interactions on {sup 19}F by PICASSO

    Energy Technology Data Exchange (ETDEWEB)

    Beltran, Berta, E-mail: bbeltran@phys.ualberta.c [Department of Physics, University of Alberta, Edmonton, T6G 2G7 (Canada)

    2010-01-01

    The PICASSO experiment at SNOLAB uses super-heated C{sub 4}F{sub 10} droplets suspended in a gel as a target sensitive to WIMP-proton spin-dependent elastic scattering. The phase II setup has been improved substantially in sensitivity by using an array of 32 detectors with an active mass of {approx}65 g each and largely reduced background. First results are presented for a subset of two detectors with target masses of {sup 19}F of 65 g and 69 g respectively and a total exposure of 13.75 {+-} 0.48 kgd. No dark matter signal was found and for WIMP masses around 24 GeV/c{sup 2} new limits have been obtained on the spin-dependent cross section on {sup 19}F of {sigma}{sub F} = 13.9 pb (90% C.L.) which can be converted into cross section limits on protons and neutrons of {sigma}{sub p} = 0.15 pb and {sigma}{sub n} = 2.45 pb respectively (90% C.L). The obtained limits on protons restrict recent interpretations of the DAMA/LIBRA annual modulations in terms of spin-dependent interactions.

  1. Output-sensitive 3D line integral convolution.

    Science.gov (United States)

    Falk, Martin; Weiskopf, Daniel

    2008-01-01

    We propose an output-sensitive visualization method for 3D line integral convolution (LIC) whose rendering speed is largely independent of the data set size and mostly governed by the complexity of the output on the image plane. Our approach of view-dependent visualization tightly links the LIC generation with the volume rendering of the LIC result in order to avoid the computation of unnecessary LIC points: early-ray termination and empty-space leaping techniques are used to skip the computation of the LIC integral in a lazy-evaluation approach; both ray casting and texture slicing can be used as volume-rendering techniques. The input noise is modeled in object space to allow for temporal coherence under object and camera motion. Different noise models are discussed, covering dense representations based on filtered white noise all the way to sparse representations similar to oriented LIC. Aliasing artifacts are avoided by frequency control over the 3D noise and by employing a 3D variant of MIPmapping. A range of illumination models is applied to the LIC streamlines: different codimension-2 lighting models and a novel gradient-based illumination model that relies on precomputed gradients and does not require any direct calculation of gradients after the LIC integral is evaluated. We discuss the issue of proper sampling of the LIC and volume-rendering integrals by employing a frequency-space analysis of the noise model and the precomputed gradients. Finally, we demonstrate that our visualization approach lends itself to a fast graphics processing unit (GPU) implementation that supports both steady and unsteady flow. Therefore, this 3D LIC method allows users to interactively explore 3D flow by means of high-quality, view-dependent, and adaptive LIC volume visualization. Applications to flow visualization in combination with feature extraction and focus-and-context visualization are described, a comparison to previous methods is provided, and a detailed performance

  2. Constraints on low-mass WIMP interactions on 19F from PICASSO

    Science.gov (United States)

    Archambault, S.; Behnke, E.; Bhattacharjee, P.; Bhattacharya, S.; Dai, X.; Das, M.; Davour, A.; Debris, F.; Dhungana, N.; Farine, J.; Gagnebin, S.; Giroux, G.; Grace, E.; Jackson, C. M.; Kamaha, A.; Krauss, C.; Kumaratunga, S.; Lafrenière, M.; Laurin, M.; Lawson, I.; Lessard, L.; Levine, I.; Levy, C.; MacDonald, R. P.; Marlisov, D.; Martin, J.-P.; Mitra, P.; Noble, A. J.; Piro, M.-C.; Podviyanuk, R.; Pospisil, S.; Saha, S.; Scallon, O.; Seth, S.; Starinski, N.; Stekl, I.; Wichoski, U.; Xie, T.; Zacek, V.

    2012-05-01

    Recent results from the PICASSO dark matter search experiment at SNOLAB are reported. These results were obtained using a subset of 10 detectors with a total target mass of 0.72 kg of 19F and an exposure of 114 kgd. The low backgrounds in PICASSO allow recoil energy thresholds as low as 1.7 keV to be obtained which results in an increased sensitivity to interactions from Weakly Interacting Massive Particles (WIMPs) with masses below 10 GeV/c2. No dark matter signal was found. Best exclusion limits in the spin dependent sector were obtained for WIMP masses of 20 GeV/c2 with a cross section on protons of σpSD=0.032 pb (90% C.L.). In the spin independent sector close to the low mass region of 7 GeV/c2 favoured by CoGeNT and DAMA/LIBRA, cross sections larger than σpSI=1.41×10-4 pb (90% C.L.) are excluded.

  3. Constraints on low-mass WIMP interactions on {sup 19}F from PICASSO

    Energy Technology Data Exchange (ETDEWEB)

    Archambault, S. [Departement de Physique, Universite de Montreal, Montreal, H3C 3J7 (Canada); Behnke, E. [Department of Physics and Astronomy, Indiana University South Bend, South Bend, IN 46634 (United States); Bhattacharjee, P.; Bhattacharya, S. [Saha Institute of Nuclear Physics, Centre for AstroParticle Physics (CAPP), Kolkata 700064 (India); Dai, X. [Department of Physics, Queen' s University, Kingston, K7L 3N6 (Canada); Das, M. [Saha Institute of Nuclear Physics, Centre for AstroParticle Physics (CAPP), Kolkata 700064 (India); Davour, A. [Department of Physics, Queen' s University, Kingston, K7L 3N6 (Canada); Debris, F. [Departement de Physique, Universite de Montreal, Montreal, H3C 3J7 (Canada); Dhungana, N.; Farine, J. [Department of Physics, Laurentian University, Sudbury, P3E 2C6 (Canada); Gagnebin, S. [Department of Physics, University of Alberta, Edmonton, T6G 2G7 (Canada); Giroux, G. [Departement de Physique, Universite de Montreal, Montreal, H3C 3J7 (Canada); Grace, E. [Department of Physics and Astronomy, Indiana University South Bend, South Bend, IN 46634 (United States); Jackson, C.M. [Departement de Physique, Universite de Montreal, Montreal, H3C 3J7 (Canada); Kamaha, A. [Department of Physics, Queen' s University, Kingston, K7L 3N6 (Canada); Krauss, C. [Department of Physics, University of Alberta, Edmonton, T6G 2G7 (Canada); and others

    2012-05-03

    Recent results from the PICASSO dark matter search experiment at SNOLAB are reported. These results were obtained using a subset of 10 detectors with a total target mass of 0.72 kg of 19F and an exposure of 114 kgd. The low backgrounds in PICASSO allow recoil energy thresholds as low as 1.7 keV to be obtained which results in an increased sensitivity to interactions from Weakly Interacting Massive Particles (WIMPs) with masses below 10 GeV/c{sup 2}. No dark matter signal was found. Best exclusion limits in the spin dependent sector were obtained for WIMP masses of 20 GeV/c{sup 2} with a cross section on protons of {sigma}{sub p}{sup SD}=0.032 pb (90% C.L.). In the spin independent sector close to the low mass region of 7 GeV/c{sup 2} favoured by CoGeNT and DAMA/LIBRA, cross sections larger than {sigma}{sub p}{sup SI}=1.41 Multiplication-Sign 10{sup -4} pb (90% C.L.) are excluded.

  4. Constraints on Low-Mass WIMP Interactions on 19F from PICASSO

    CERN Document Server

    Archambault, S; Bhattacharjee, P; Bhattacharya, S; Dai, X; Das, M; Davour, A; Debris, F; Dhungana, N; Farine, J; Gagnebin, S; Giroux, G; Grace, E; Jackson, C M; Kamaha, A; Krauss, C; Kumaratunga, S; Lafrenière, M; Laurin, M; Lawson, I; Lessard, L; Levine, I; Levy, C; MacDonald, R P; Marlisov, D; Martin, J -P; Mitra, P; Noble, A J; Piro, M -C; Podviyanuk, R; Pospisil, S; Saha, S; Scallon, O; Seth, S; Starinski, N; Stekl, I; Wichoski, U; Xie, T; Zacek, V

    2012-01-01

    Recent results from the PICASSO dark matter search experiment at SNOLAB are reported. These results were obtained using a subset of 10 detectors with a total target mass of 0.72 kg of 19F and an exposure of 114 kgd. The low backgrounds in PICASSO allow recoil energy thresholds as low as 1.7 keV to be obtained which results in an increased sensitivity to interactions from Weakly Interacting Massive Particles (WIMPs) with masses below 10 GeV/c^2. No dark matter signal was found. Best exclusion limits in the spin dependent sector were obtained for WIMP masses of 20 GeV/c^2 with a cross section on protons of sigma_p^SD = 0.032 pb (90% C.L.). In the spin independent sector close to the low mass region of 7 GeV/c2 favoured by CoGeNT and DAMA/LIBRA, cross sections larger than sigma_p^SI = 1.41x10^-4 pb (90% C.L.) are excluded.

  5. The zero age main sequence of WIMP burners

    Science.gov (United States)

    Fairbairn, Malcolm; Scott, Pat; Edsjö, Joakim

    2008-02-01

    We modify a stellar structure code to estimate the effect upon the main sequence of the accretion of weakly-interacting dark matter onto stars and its subsequent annihilation. The effect upon the stars depends upon whether the energy generation rate from dark matter annihilation is large enough to shut off the nuclear burning in the star. Main sequence weakly-interacting massive particles (WIMP) burners look much like proto-stars moving on the Hayashi track, although they are in principle completely stable. We make some brief comments about where such stars could be found, how they might be observed and more detailed simulations which are currently in progress. Finally we comment on whether or not it is possible to link the paradoxically hot, young stars found at the galactic center with WIMP burners.

  6. RC-IAL cell line: sensitivity of rubella virus grow

    Directory of Open Access Journals (Sweden)

    Figueiredo Cristina A

    2000-01-01

    Full Text Available OBJECTIVE: The rapid growth of the rubella virus in RC-IAL² with development of cytopathic effect, in response to rubella virus infection, is described. For purposes of comparison, the rubella virus RA-27/3 strain was titered simultaneously in the RC-IAL, Vero, SIRC and RK13 cell lines. METHODS: Rubella virus RA-27/3 strain are inoculated in the RC-IAL cell line (rabbit Kidney, Institute Adolfo Lutz. Plates containing 1.5x10(5 cells/ml of RC-IAL line were inoculated with 0.1ml s RA-27/3 strain virus containing 1x 10(4TCID50/0.1ml. A 25% cytopathic effect was observed after 48 hours and 100% after 96 hours. The results obtained were compared to those observed with the SIRC, Vero and RK13 cell lines. Rubella virus was detected by immunohistochemistry. RESULTS: With the results, it was possible to conclude that the RC-IAL cell line is a very good substrate for culturing rubella virus. The cells inoculated with rubella virus were examined by phase contrast microscopy and showed the characteristic rounded, bipolar and multipolar cells. The CPE in RC-IAL was observed in the first 48 hours and the curve of the increased infectivity was practically the same as observed in other cell lines. CONCLUSIONS: These findings are important since this is one the few cell lines described in the literature with a cytopathic effect. So it can be used for antigen preparation and serological testing for the diagnosis of specific rubella antibodies.

  7. A Search for WIMP Dark Matter using an Optimized Chi-square Technique on the Final Data from the Cryogenic Dark Matter Search Experiment (CDMS II)

    Science.gov (United States)

    Manungu Kiveni, Joseph

    2012-06-01

    During the last two decades, cosmology has become a precision observational science thanks (in part) to the incredible number of experiments performed to better understand the composition of the universe. The large amount of data accumulated strongly indicates that the bulk of the universe's matter is in the form of non-baryonic matter that does not interact electromagnetically. Combined evidence from the dynamics of galaxies and galaxy clusters confirms that most of the mass in the universe is not composed of any known form of matter. Measurements of the cosmic microwave background, big bang nucleosynthesis and many other experiments indicate that ˜80% of the matter in the universe is dark, non-relativistic and cold. The dark matter resides in the halos surrounding galaxies, galaxy clusters and other large-scale structures. Weakly Interacting Massive Particles (WIMPs) are well motivated class of dark matter candidates that arise naturally in supersymmetric extensions to the Standard Model of particles physics, and can be produced as non-relativistic thermal relics in the early universe with about the right density to account for the missing mass. The Cryogenic Dark Matter Search (CDMS) experiment seeks to directly detect the keV-scale energy deposited by WIMPs in the galactic halo when they scatter from nuclei in the crystalline detectors made of germanium and silicon. These detectors, called Z-sensitive Ionization and Phonon detectors (ZIPs) are operated at ˜ 45 mK and simultaneously measure the ionization and the (athermal) phonons produced by particle interactions. The ratio of ionization and phonon energies allows discrimination of a low rate of nuclear recoils (expected for WIMPs) from an overwhelming rate of electron recoils (expected for most backgrounds). Phonon-pulse shape and timing enables further suppression of lower-rate interactions at the detector surfaces. This dissertation describes the results of a WIMP search using CDMS II data sets

  8. 5th Patras workshop on axions, WIMPs and WISPs (PATRAS 2009). Proceedings

    Energy Technology Data Exchange (ETDEWEB)

    Jaeckel, Joerg; Lindner, Axel; Redondo, Javier (eds.)

    2010-06-15

    The following topics were dealt with: Direct searches for dark matter, indirect searches for WIMPS, photon generation and laser polarization experiments, direct axion signals, theoretic WISP developments. (HSI)

  9. A Search for WIMP Dark Matter Using an Optimized Chi-square Technique on the Final Data from the Cryogenic Dark Matter Search Experiment (CDMS II)

    Energy Technology Data Exchange (ETDEWEB)

    Manungu Kiveni, Joseph [Syracuse Univ., NY (United States)

    2012-12-01

    This dissertation describes the results of a WIMP search using CDMS II data sets accumulated at the Soudan Underground Laboratory in Minnesota. Results from the original analysis of these data were published in 2009; two events were observed in the signal region with an expected leakage of 0.9 events. Further investigation revealed an issue with the ionization-pulse reconstruction algorithm leading to a software upgrade and a subsequent reanalysis of the data. As part of the reanalysis, I performed an advanced discrimination technique to better distinguish (potential) signal events from backgrounds using a 5-dimensional chi-square method. This dataanalysis technique combines the event information recorded for each WIMP-search event to derive a backgrounddiscrimination parameter capable of reducing the expected background to less than one event, while maintaining high efficiency for signal events. Furthermore, optimizing the cut positions of this 5-dimensional chi-square parameter for the 14 viable germanium detectors yields an improved expected sensitivity to WIMP interactions relative to previous CDMS results. This dissertation describes my improved (and optimized) discrimination technique and the results obtained from a blind application to the reanalyzed CDMS II WIMP-search data.

  10. Goodbye to WIMPs: A Scalable Interface for ALMA Operations

    Science.gov (United States)

    Schwarz, J.; Pietriga, E.; Schilling, M.; Grosbol, P.

    2011-07-01

    The operators of the ALMA Observatory will monitor and control more than 50 mm/submm radio antennas and their associated instrumentation from an operations site that is separated from this hardware by 35-50 km. Software that enables them to identify trouble spots and react to failures quickly in this environment will be critical to the safe and efficient functioning of the observatory. Early commissioning of ALMA uses a operator interface implemented with a standard window, icon, menu, pointing device (WIMP) toolkit. Early experience indicates that this paradigm will not scale well as the number of antennas approaches its full complement. Operators lose time as they manipulate overlapping or tabbed windows to drill-down to detailed diagnostic data, losing a feeling for "where they are" in the process. The WIMP model reaches its limits when there is so much information to present to users that they cannot focus on details while maintaining a view from above. To simplify the operators' tasks and let them concentrate on the real issues at hand rather than continually re-organizing their use of screen space, we are replacing the existing top-level interface with a multi-scale interface that takes advantage of semantic zooming, dynamic network visualization and other advanced filtering, navigation and visualization features. Following the first of several planned participatory design workshops, we have developed prototypes to show how users' needs can be met with the kinds of navigation that become possible when the restrictions of the WIMP model are lifted. Cycles of design and implementation coupled with active user feedback will characterize this project up through deployment.

  11. Research paper citation record keeping: it is not for wimps.

    Science.gov (United States)

    Dodson, M V

    2008-10-01

    In an era in which academic institutions are held more accountable, a problem, which does not seem to have an immediate resolution, exists: how does one demonstrate the quality of one's work? Specifically, how does one show that his or her research is making an impact and is thought to be important? A variety of measures of scientific journal article importance, reflected mostly by citation analyses, have been focused on as being potential gold standards. However, although several measures have been reported, keeping a record of citation of individual research papers, primarily due to the effort involved in finding accurate information, is not for wimps.

  12. Consistency of WIMP Dark Matter as radiative neutrino mass messenger

    Energy Technology Data Exchange (ETDEWEB)

    Merle, Alexander [Max-Planck-Institut für Physik (Werner-Heisenberg-Institut),Föhringer Ring 6, 80805 München (Germany); Platscher, Moritz [Max-Planck-Institut für Kernphysik,Saupfercheckweg 1, 69117 Heidelberg (Germany); Rojas, Nicolás; Valle, José W.F.; Vicente, Avelino [AHEP Group, Instituto de Física Corpuscular - C.S.I.C./Universitat de València,Parc Cientific de Paterna, C/Catedratico José Beltrán, 2 E-46980 Paterna (València) (Spain)

    2016-07-04

    The scotogenic scenario provides an attractive approach to both Dark Matter and neutrino mass generation, in which the same symmetry that stabilises Dark Matter also ensures the radiative seesaw origin of neutrino mass. However the simplest scenario may suffer from inconsistencies arising from the spontaneous breaking of the underlying ℤ{sub 2} symmetry. Here we show that the singlet-triplet extension of the simplest model naturally avoids this problem due to the presence of scalar triplets neutral under the ℤ{sub 2} which affect the evolution of the couplings in the scalar sector. The scenario offers good prospects for direct WIMP Dark Matter detection through the nuclear recoil method.

  13. Readout technologies for directional WIMP Dark Matter detection

    Energy Technology Data Exchange (ETDEWEB)

    Battat, J.B.R., E-mail: jbattat@wellesley.edu [Department of Physics, Wellesley College, 106 Central Street, Wellesley, MA 02481 (United States); Irastorza, I.G. [Grupo de Física Nuclear y Astropartículas, Departamento de Física Teórica, Universidad de Zaragoza, 50009 Zaragoza (Spain); Aleksandrov, A. [Istituto Nazionale di Fisica Nucleare, Sezione di Napoli, Naples (Italy); Asada, T. [Nagoya University, J-464-8602 Nagoya (Japan); Baracchini, E. [Istituto Nazionale di Fisica Nucleare, Laboratori Nazionali di Frascati, Frascati (Italy); Billard, J. [Laboratoire de Physique Subatomique et de Cosmologie, Université Grenoble Alpes, CNRS/IN2P3, 53, avenue des Martyrs, Grenoble (France); IPNL, Université de Lyon, Université Lyon 1, CNRS/IN2P3, 4 rue E. Fermi 69622 Villeurbanne cedex (France); Bosson, G.; Bourrion, O.; Bouvier, J. [Laboratoire de Physique Subatomique et de Cosmologie, Université Grenoble Alpes, CNRS/IN2P3, 53, avenue des Martyrs, Grenoble (France); Buonaura, A. [Istituto Nazionale di Fisica Nucleare, Sezione di Napoli, Naples (Italy); Dipartimento di Fisica, Università Federico II, Napoli, I-80125 Napoli (Italy); Burdge, K. [Laboratory for Nuclear Science, Massachusetts Institute of Technology, Cambridge, MA 02139 (United States); Caltech Division of Physics, Mathematics, and Astronomy, Pasadena, CA (United States); Cebrián, S. [Grupo de Física Nuclear y Astropartículas, Departamento de Física Teórica, Universidad de Zaragoza, 50009 Zaragoza (Spain); and others

    2016-11-29

    The measurement of the direction of WIMP-induced nuclear recoils is a compelling but technologically challenging strategy to provide an unambiguous signature of the detection of Galactic dark matter. Most directional detectors aim to reconstruct the dark-matter-induced nuclear recoil tracks, either in gas or solid targets. The main challenge with directional detection is the need for high spatial resolution over large volumes, which puts strong requirements on the readout technologies. In this paper we review the various detector readout technologies used by directional detectors. In particular, we summarize the challenges, advantages and drawbacks of each approach, and discuss future prospects for these technologies.

  14. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Florida Panhandle: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for the Florida...

  15. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: New Hampshire: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for New...

  16. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Hudson River: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for the Hudson...

  17. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Central California: FISHL (Fish Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for anadromous fish and rare fish species occurrences in Central California. Vector lines in this data set...

  18. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Central California: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for Central...

  19. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Southern California: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for Southern...

  20. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Mississippi: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for Mississippi....

  1. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Northern California: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for Northern...

  2. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: North Carolina: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for North...

  3. Aleutian Islands Coastal Resources Inventory and Environmental Sensitivity Maps: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains lines and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for the Aleutian...

  4. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Southern California: FISHL (Fish Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for threatened/endandered/rare and/or anadromous fish species in Southern California. Vector lines in this...

  5. Virginia ESI: ESI (Environmental Sensitivity Index Shoreline Types - Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing the shoreline and coastal habitats for Virginia, classified according to the Environmental Sensitivity...

  6. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Northern California: FISHL (Fish Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for anadromous and threatened/endangered stream species in Northern California. Vector lines in this data...

  7. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: South Florida: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for South...

  8. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: South Florida: ESIL (ESI Shoreline Types - Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The ESIL data set contains vector lines representing the shoreline and coastal habitats of South Florida classified according to the Environmental Sensitivity Index...

  9. Supersymmetry with Radiatively-Driven Naturalness: Implications for WIMP and Axion Searches

    Directory of Open Access Journals (Sweden)

    Kyu Jung Bae

    2015-05-01

    Full Text Available By insisting on naturalness in both the electroweak and quantum chromodynamics (QCD sectors of the minimal supersymmetric standard model (MSSM, the portrait for dark matter production is seriously modified from the usual weakly interacting massive particle (WIMP miracle picture. In supersymmetry (SUSY models with radiatively-driven naturalness (radiative natural SUSY or radiative natural SUSY (RNS which include a Dine–Fischler–Srednicki–Zhitnitsky (DFSZ-like solution to the strong charge-conjugation-parity (CP and SUSY \\(\\mu\\ problems, dark matter is expected to be an admixture of both axions and higgsino-like WIMPs. The WIMP/axion abundance calculation requires simultaneous solution of a set of coupled Boltzmann equations which describe quasi-stable axinos and saxions. In most of parameter space, axions make up the dominant contribution of dark matter although regions of WIMP dominance also occur. We show the allowed range of Peccei-Quinn (PQ scale \\(f_a\\ and compare to the values expected to be probed by the axion dark matter search experiment (ADMX axion detector in the near future. We also show WIMP detection rates, which are suppressed from usual expectations, because now WIMPs comprise only a fraction of the total dark matter. Nonetheless, ton-scale noble liquid detectors should be able to probe the entirety of RNS parameter space. Indirect WIMP detection rates are less propitious since they are reduced by the square of the depleted WIMP abundance.

  10. Limits on WIMP cross-sections from the NAIAD experiment at the Boulby Underground Laboratory

    CERN Document Server

    Alner, G J; Arnison, G J; Barton, J C; Bewick, A; Bungau, C; Camanzi, B; Carson, M J; Davidge, D; Daw, E; Dawson, J V; Davies, G J; Davies, J C; Duffy, C; Durkin, T J; Gamble, T; Hart, S P; Hollingworth, R; Homer, G J; Howard, A S; Ivaniouchenkov, Yu; Jones, W G; Joshi, M K; Kirkpatrick, J; Kudryavtsev, V A; Lawson, T B; Lebedenko, V; Lehner, M J; Lewin, J D; Lightfoot, P K; Liubarsky, I; Lüscher, R; McMillan, J E; Morgan, B; Murphy, A; Nickolls, A; Nicklin, G; Paling, S M; Preece, R M; Quenby, J J; Roberts, J W; Robinson, M; Smith, N J T; Smith, P F; Spooner, N J C; Sumner, T J; Tovey, Daniel R; Tziaferi, E

    2005-01-01

    The NAIAD experiment (NaI Advanced Detector) for WIMP dark matter searches at the Boulby Underground Laboratory (North Yorkshire, UK) ran from 2000 until 2003. A total of 44.9 kg x years of data collected with 2 encapsulated and 4 unencapsulated NaI(Tl) crystals with high light yield were included in the analysis. We present final results of this analysis carried out using pulse shape discrimination. No signal associated with nuclear recoils from WIMP interactions was observed in any run with any crystal. This allowed us to set upper limits on the WIMP-nucleon spin-independent and WIMP-proton spin-dependent cross-sections. The NAIAD experiment has so far imposed the most stringent constraints on the spin-dependent WIMP-proton cross-section.

  11. WIMP search and a Cherenkov detector prototype for ILC polarimetry

    Energy Technology Data Exchange (ETDEWEB)

    Bartels, Christoph

    2011-10-15

    The planned International Linear Collider (ILC) will be an essential experiment to precisely determine the properties and structure of physics at the TeV scale. An important feature of the ILC is the possibility to use polarized electrons and positrons. In part 1 of this thesis, a model independent search for Weakly Interacting Massive Particles (WIMPs) at ILC is presented. The signal channel under study is direct WIMP pair production with associated Initial State Radiation (ISR), e{sup +}e{sup -} {yields} {chi}{chi}{gamma}, where the WIMPs leave the detector without any further interaction, and only the emitted photon is detected. From the energy spectrum of the detected photons the coupling structure, cross sections, masses and the quantum number of the dominant partial wave in the production process can be inferred. The analysis includes the dominant SM, as well as machine-induced backgrounds, and is performed using a full simulation of the ILD detector concept. For an integrated luminosity of L=500 fb{sup -1}, the signal cross sections can be measured to a precision of 3%, dominated by systematic uncertainties on the polarization measurement of the initial electrons and positrons. Masses can be measured to a precision of up to 2% by a comparison of the data photon spectrum to parametrized template spectra. In part 2 of this thesis, a Cherenkov detector prototype for Compton polarimetry at ILC is presented. For the polarization measurement a systematic uncertainty of {delta} P/P = 0.25% or better is envisioned. To achieve this goal, the Cherenkov detector has to be precisely aligned with the fan of Compton scattered electrons and its signal response needs to be highly linear. For the detector prototype data driven alignment strategies have been developed by comparing data recorded at the Elsa accelerator in Bonn, Germany, with detailed Geant4 simulations. With the use of multi-anode photomultipliers, data driven alignment strategies promise to provide the

  12. Study of WIMP annihilations into a pair of on-shell scalar mediators

    Science.gov (United States)

    Jia, Lian-Bao

    2016-11-01

    In this article, we focus on new scalar ϕ mediated scalar/vectorial weakly interacting massive particles (WIMPs) with ϕ 's mass slightly below the WIMP mass. To explain the Galactic center 1-3 GeV gamma-ray excess, here we consider the case that a WIMP pair predominantly annihilates into an on-shell ϕ ϕ pair with ϕ mainly decaying to τ τ ¯. The masses of WIMPs are in a range about 14-22 GeV, and the annihilations of WIMPs are phase space suppressed today. In this annihilation scheme, the couplings of the ϕ -standard model (SM) particles are almost arbitrarily small, and the WIMP-nucleus spin-independent scattering can be tolerant by the present dark matter (DM) direct detections. A scalar mediator-Higgs field mixing is introduced, which is small and available. The lower limit on the couplings of the ϕ -SM particles set by the thermal equilibrium in the early Universe is derived, and this constraint is above the neutrino background for scalar DM in direct detections. The WIMPs may be detectable at the upgraded DM direct detection experiment in the next few years, and the exotic decay h →ϕ ϕ , the production of ϕ may be observable at the future high-luminosity e+e- collider.

  13. Beyond WIMPs: the Quark (Anti Nugget Dark Matter

    Directory of Open Access Journals (Sweden)

    Zhitnitsky Ariel

    2017-01-01

    Full Text Available We review a testable dark matter (DM model outside of the standard WIMP paradigm. The model is unique in a sense that the observed ratio Ωdark ≃ Ωvisible for visible and dark matter densities finds its natural explanation as a result of their common QCD origin when both types of matter (DM and visible are formed during the QCD phase transition and both are proportional to single dimensional parameter of the system, Λqcd. We argue that the charge separation effect also inevitably occurs during the same QCD phase transition in the presence of the CP odd axion field a(x. It leads to preferential formation of one species of nuggets on the scales of the visible Universe where the axion field a(x is coherent. A natural outcome of this preferential evolution is that only one type of the visible baryons (not anti- baryons remain in the system after the nuggets complete their formation. Unlike conventional WIMP dark matter candidates, the nuggets and anti-nuggets are strongly interacting but macroscopically large objects. The rare events of annihilation of the anti-nuggets with visible matter lead to a number of observable effects. We argue that the relative intensities for a number of measured excesses of emission from the centre of galaxy (covering more than 11 orders of magnitude are determined by standard and well established physics. At the same time the absolute intensity of emission is determined by a single new fundamental parameter of the theory, the axion mass, 10−6eV ≲ ma ≲ 10−3eV. Finally, we comment on implications of these studies for the axion search experiments, including microwave cavity and the Orpheus experiments.

  14. Beyond WIMPs: the Quark (Anti) Nugget Dark Matter

    Science.gov (United States)

    Zhitnitsky, Ariel

    2017-03-01

    We review a testable dark matter (DM) model outside of the standard WIMP paradigm. The model is unique in a sense that the observed ratio Ωdark ≃ Ωvisible for visible and dark matter densities finds its natural explanation as a result of their common QCD origin when both types of matter (DM and visible) are formed during the QCD phase transition and both are proportional to single dimensional parameter of the system, Λqcd. We argue that the charge separation effect also inevitably occurs during the same QCD phase transition in the presence of the CP odd axion field a(x). It leads to preferential formation of one species of nuggets on the scales of the visible Universe where the axion field a(x) is coherent. A natural outcome of this preferential evolution is that only one type of the visible baryons (not anti- baryons) remain in the system after the nuggets complete their formation. Unlike conventional WIMP dark matter candidates, the nuggets and anti-nuggets are strongly interacting but macroscopically large objects. The rare events of annihilation of the anti-nuggets with visible matter lead to a number of observable effects. We argue that the relative intensities for a number of measured excesses of emission from the centre of galaxy (covering more than 11 orders of magnitude) are determined by standard and well established physics. At the same time the absolute intensity of emission is determined by a single new fundamental parameter of the theory, the axion mass, 10-6eV ≲ ma ≲ 10-3eV. Finally, we comment on implications of these studies for the axion search experiments, including microwave cavity and the Orpheus experiments.

  15. Limits on light WIMPs from the CDEX-1 experiment with a p-type point-contact germanium detector at the China Jingping Underground Laboratory

    CERN Document Server

    Yue, Q; Kang, K J; Cheng, J P; Li, Y J; Lin, S T; Chang, J P; Chen, N; Chen, Q H; Chen, Y H; Chuang, Y C; Deng, Z; Du, Q; Gong, H; Hao, X Q; He, H J; He, Q J; Huang, H X; Huang, T R; Jiang, H; Li, H B; Li, J M; Li, J; Li, X; Li, X Y; Li, Y L; Liao, H Y; Lin, F K; Liu, S K; Lv, L C; Ma, H; Mao, S J; Qin, J Q; Ren, J; Ruan, X C; Shen, M B; Singh, L; Singh, M K; Soma, A K; Su, J; Tang, C J; Tseng, C H; Wang, J M; Wang, L; Wang, Q; Wong, H T; Wu, S Y; Wu, Y C; Xianyu, Z Z; Xiao, R Q; Xing, H Y; Xu, F Z; Xu, Y; Xu, X J; Xue, T; Yang, L T; Yang, S W; Yi, N; Yu, C X; Yu, H; Yu, X Z; Zeng, X H; Zeng, Z; Zhang, L; Zhang, Y H; Zhao, M G; Zhou, Z Y; Zhu, J J; Zhu, W B; Zhu, X Z; Zhu, Z H

    2014-01-01

    We report results of a search for light Dark Matter WIMPs with CDEX-1 experiment at the China Jingping Underground Laboratory, based on 53.9 kg-days of data from a p-type point-contact germanium detector enclosed by a NaI(Tl) crystal scintillator as anti-Compton detector. All events above the analysis threshold of 475 eVee can be quantitatively accounted for with the understood background channels, and there is no excess of residual events. An order of magnitude improvement in the sensitivities of spin-independent elastic cross-section over our previous results is achieved. Part of the allowed regions at WIMP mass of 6-20 GeV are probed and excluded.

  16. Search for gamma-ray spectral lines with the Fermi Large Area Telescope and dark matter implications

    Energy Technology Data Exchange (ETDEWEB)

    Ackermann, M.; Ajello, M.; Albert, A.; Allafort, A.; Baldini, L.; Barbiellini, G.; Bastieri, D.; Bechtol, K.; Bellazzini, R.; Bissaldi, E.; Bloom, E. D.; Bonamente, E.; Bottacini, E.; Brandt, T. J.; Bregeon, J.; Brigida, M.; Bruel, P.; Buehler, R.; Buson, S.; Caliandro, G. A.; Cameron, R. A.; Caraveo, P. A.; Casandjian, J. M.; Cecchi, C.; Charles, E.; Chaves, R. C. G.; Chekhtman, A.; Chiang, J.; Ciprini, S.; Claus, R.; Cohen-Tanugi, J.; Conrad, J.; Cutini, S.; D’Ammando, F.; de Angelis, A.; de Palma, F.; Dermer, C. D.; Digel, S. W.; Di Venere, L.; Drell, P. S.; Drlica-Wagner, A.; Essig, R.; Favuzzi, C.; Fegan, S. J.; Ferrara, E. C.; Focke, W. B.; Franckowiak, A.; Fukazawa, Y.; Funk, S.; Fusco, P.; Gargano, F.; Gasparrini, D.; Germani, S.; Giglietto, N.; Giordano, F.; Giroletti, M.; Glanzman, T.; Godfrey, G.; Gomez-Vargas, G. A.; Grenier, I. A.; Guiriec, S.; Gustafsson, M.; Hadasch, D.; Hayashida, M.; Hill, A. B.; Horan, D.; Hou, X.; Hughes, R. E.; Inoue, Y.; Izaguirre, E.; Jogler, T.; Kamae, T.; Knödlseder, J.; Kuss, M.; Lande, J.; Larsson, S.; Latronico, L.; Longo, F.; Loparco, F.; Lovellette, M. N.; Lubrano, P.; Malyshev, D.; Mayer, M.; Mazziotta, M. N.; McEnery, J. E.; Michelson, P. F.; Mitthumsiri, W.; Mizuno, T.; Moiseev, A. A.; Monzani, M. E.; Morselli, A.; Moskalenko, I. V.; Murgia, S.; Nakamori, T.; Nemmen, R.; Nuss, E.; Ohsugi, T.; Okumura, A.; Omodei, N.; Orienti, M.; Orlando, E.; Ormes, J. F.; Paneque, D.; Perkins, J. S.; Pesce-Rollins, M.; Piron, F.; Pivato, G.; Rainò, S.; Rando, R.; Razzano, M.; Razzaque, S.; Reimer, A.; Reimer, O.; Romani, R. W.; Sánchez-Conde, M.; Schulz, A.; Sgrò, C.; Siegal-Gaskins, J.; Siskind, E. J.; Snyder, A.; Spandre, G.; Spinelli, P.; Suson, D. J.; Tajima, H.; Takahashi, H.; Thayer, J. G.; Thayer, J. B.; Tibaldo, L.; Tinivella, M.; Tosti, G.; Troja, E.; Uchiyama, Y.; Usher, T. L.; Vandenbroucke, J.; Vasileiou, V.; Vianello, G.; Vitale, V.; Winer, B. L.; Wood, K. S.; Wood, M.; Yang, Z.; Zaharijas, G.; Zimmer, S.

    2013-10-22

    Weakly interacting massive particles (WIMPs) are a theoretical class of particles that are excellent dark matter candidates. WIMP annihilation or decay may produce essentially monochromatic γ rays detectable by the Fermi Large Area Telescope (LAT) against the astrophysical γ -ray emission of the Galaxy. We have searched for spectral lines in the energy range 5–300 GeV using 3.7 years of data, reprocessed with updated instrument calibrations and an improved energy dispersion model compared to the previous Fermi-LAT Collaboration line searches. We searched in five regions selected to optimize sensitivity to different theoretically motivated dark matter density distributions. We did not find any globally significant lines in our a priori search regions and present 95% confidence limits for annihilation cross sections of self-conjugate WIMPs and decay lifetimes. Our most significant fit occurred at 133 GeV in our smallest search region and had a local significance of 3.3 standard deviations, which translates to a global significance of 1.5 standard deviations. We discuss potential systematic effects in this search, and examine the feature at 133 GeV in detail. We find that the use both of reprocessed data and of additional information in the energy dispersion model contributes to the reduction in significance of the linelike feature near 130 GeV relative to significances reported in other works. We also find that the feature is narrower than the LAT energy resolution at the level of 2 to 3 standard deviations, which somewhat disfavors the interpretation of the 133 GeV feature as a real WIMP signal.

  17. Chicken lines divergently selected for antibody responses to sheep red blood cells show line-specific differences in sensitivity to immunomodulation by diet. Part I: Humoral parameters

    NARCIS (Netherlands)

    Adriaansen-Tennekes, R.; Vries Reilingh, de G.; Nieuwland, M.G.B.; Parmentier, H.K.; Savelkoul, H.F.J.

    2009-01-01

    Individual differences in nutrient sensitivity have been suggested to be related with differences in stress sensitivity. Here we used layer hens divergently selected for high and low specific antibody responses to SRBC (i.e., low line hens and high line hens), reflecting a genetically based

  18. WIMP dark matter from gravitino decays and leptogenesis

    Energy Technology Data Exchange (ETDEWEB)

    Buchmueller, W.; Domcke, V.; Schmitz, K.

    2012-03-15

    The spontaneous breaking of B-L symmetry naturally accounts for the small observed neutrino masses via the seesaw mechanism. We have recently shown that the cosmological realization of B-L breaking in a supersymmetric theory can successfully generate the initial conditions of the hot early universe, i.e. entropy, baryon asymmetry and dark matter, if the gravitino is the lightest superparticle (LSP). This implies relations between neutrino and superparticle masses. Here we extend our analysis to the case of very heavy gravitinos which are motivated by hints for the Higgs boson at the LHC. We find that the nonthermal production of 'pure' wino or higgsino LSPs, i.e. weakly interacting particles (WIMPs), in heavy gravitino decays can account for the observed amount of dark matter while simultaneously fulfilling the constraints imposed by primordial nucleosynthesis and leptogenesis within a range of LSP, gravitino and neutrino masses. For instance, a mass of the lightest neutrino of 0.05 eV would require a higgsino mass below 900 GeV and a gravitino mass of at least 20 TeV.

  19. Spin-dependent WIMP-nucleon cross section limits from first data of PandaX-II experiment

    CERN Document Server

    Fu, Changbo; Zhou, Xiaopeng; Chen, Xun; Chen, Yunhua; Fang, Deqing; Giboni, Karl; Giuliani, Franco; Han, Ke; Huang, Xingtao; Ji, Xiangdong; Ju, Yonglin; Lei, Siao; Li, Shaoli; Liu, Huaxuan; Liu, Jianglai; Ma, Yugang; Mao, Yajun; Ren, Xiangxiang; Tan, Andi; Wang, Hongwei; Wang, Jiming; Wang, Meng; Wang, Qiuhong; Wang, Siguang; Wang, Xuming; Wang, Zhou; Wu, Shiyong; Xiao, Mengjiao; Xie, Pengwei; Yan, Binbin; Yang, Yong; Yue, Jianfeng; Zhang, Hongguang; Zhang, Tao; Zhao, Li; Zhou, Ning

    2016-01-01

    We report new constrains on the spin-dependent WIMP-neutron and WIMP-proton cross sections using recently released data from the PandaX-II experiment, a dual phase liquid xenon dark matter experiment at the China JinPing Underground Laboratory, with a total exposure of 3.3$\\times10^4$ kg-day. Assuming a standard axial-vector spin-dependent WIMP interaction with $^{129}$Xe and $^{131}$Xe nuclei, the most stringent upper limits on WIMP-neutron cross sections for WIMPs with masses above 10 GeV/c$^{2}$ are set in all direct detection experiments, with a minimum upper limit of $4.1\\times 10^{-41}$ cm$^2$ at 90\\% confidence level for a WIMP mass of 40 GeV/c$^{2}$, representing more than a factor of two improvement on the best available limits at high masses.

  20. Interferon-gamma sensitizes colonic epithelial cell lines to physiological and therapeutic inducers of colonocyte apoptosis.

    LENUS (Irish Health Repository)

    O'Connell, J

    2012-02-03

    Homeostasis in the colonic epithelium is achieved by a continuous cycle of proliferation and apoptosis, in which imbalances are associated with disease. Inflammatory bowel disease (IBD) and colon cancer are associated with either excessive or insufficient apoptosis of colonic epithelial cells, respectively. By using two colonic epithelial cell lines, HT29 and SW620, we investigated how the epithelial cell\\'s sensitivity to apoptosis was regulated by the proinflammatory cytokine interferon-gamma (IFN-gamma). We found that IFN-gamma sensitized HT29 cells, and to a lesser extent SW620, to diverse inducers of apoptosis of physiologic or therapeutic relevance to the colon. These apoptosis inducers included Fas (CD95\\/APO-1) ligand (FasL), short-chain fatty acids, and chemotherapeutic drugs. The extent of IFN-gamma-mediated apoptosis sensitization in these two cell lines correlated well with the degree of IFN-gamma-mediated upregulation of the proapoptotic protease caspase-1. Although IFN-gamma alone effectively sensitized HT29 cells to apoptosis, inclusion of the protein synthesis inhibitor cyclohexamide (CHX) during apoptotic challenge was necessary for maximal sensitization of SW620. The requirement of CHX to sensitize SW620 cells to apoptosis implies a need to inhibit translation of antiapoptotic proteins absent from HT29. In particular, the antiapoptotic protein Bcl-2 was strongly expressed in SW620 cells but absent from HT29. Our results indicate that IFN-gamma increases the sensitivity of colonic epithelial cells to diverse apoptotic stimuli in concert, via upregulation of caspase-1. Our findings implicate caspase-1 and Bcl-2 as important central points of control determining the general sensitivity of colonic epithelial cells to apoptosis.

  1. [Establishment and characterization of human ovarian fibrosarcoma cell line and its sensitivity to anticancer agents].

    Science.gov (United States)

    Kiyozuka, Y; Nishimura, H; Iwanaga, S; Yakushiji, M; Ito, K; Nakano, S; Tamori, N; Adachi, S; Noda, T; Imai, S

    1992-04-01

    We succeeded in establishing a cell line (KEN-3) for subculture from a fibrosarcoma which originated in the ovary in a girl aged 17 years. Its characteristics and sensitivity to anticancer agents are reported in this paper. 1. Characteristics of established cell line. Lined cells consist of multinucleated giant cells mixed among many spindle-shaped cells. They grow in small colonies and have none of the pavement-like arrangement characteristic of epithelial tumor cells. The number of chromosomes ranged from 45 to 128 (mode: pseudo-triploidy region, 65). The doubling time, cellular density and plating efficiency were 76.9 hours, 5.4 x 10(5)/cm2 and 30.2%, respectively. Concerning tumor markers, CEA and sialyl SSEA-1 were only produced in small quantities. Subculture was possible subcutaneously in the nude mouse with no capacity for the production of ascites. 2. Susceptibility to anticancer agents and GP170 expression. The in vitro susceptibility to about 12 types of anticancer agents was investigated with the MTT assay. IC50/PPC was shown to be less than 1 for Adriamycin only. The sensitivity to CDDP (IC50/PPC: 4.8) was low, and no sensitivity was observed at all to DTIC, which is used frequently for mesenchymal tumors. GP170 (mdr-1 products) was positive in established cells in immunohistochemical stain.

  2. Comparative sensitivity of four different cell lines for the isolation of Coxiella burnetii.

    Science.gov (United States)

    Lockhart, Michelle G; Islam, Aminul; Fenwick, Stan G; Graves, Stephen R; Stenos, John

    2012-09-01

    Coxiella burnetii is an obligate intracellular bacterium that causes the disease Q-fever. This is usually diagnosed by serology (immunofluorescence assay) and/or PCR detection of C. burnetii DNA. However, neither of these methods can determine the viability of the bacterium. Four different cell lines were compared for their ability to amplify very low numbers of viable C. burnetii. Two different isolates of C. burnetii were used. For the Henzerling isolate, DH82 (dog macrophage) cells were the most sensitive with an ID (50) (dose required to infect 50% of cell cultures) of 14.6 bacterial copies. For the Arandale isolate, Vero (monkey epithelial) cells were the most sensitive with an ID (50) of less than one bacterium in a 100-μL inoculum. The Vero cell line appeared highly useful as vacuoles could be seen microscopically in unstained infected cells. The findings of this study favour the use of Vero and DH82 tissue culture cell lines for isolation and growth of C. burnetii in vitro. The other cell lines, XTC-2 and L929, were less suitable. © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

  3. CELLULAR BASIS FOR DIFFERENTIAL SENSITIVITY TO CISPLATIN IN HUMAN GERM-CELL TUMOR AND COLON-CARCINOMA CELL-LINES

    NARCIS (Netherlands)

    SARK, MWJ; TIMMERBOSSCHA, H; MEIJER, C; UGES, DRA; SLUITER, WJ; PETERS, WHM; MULDER, NH; DEVRIES, EGE

    Cisplatin (CDDP) resistance mechanisms were studied in a model of three germ cell tumour and three colon carcinoma cell lines representing intrinsically CDDP-sensitive and -resistant tumours respectively. The CDDP sensitivity of the cell lines mimicked the clinical situation. The glutathione levels

  4. Joint Rome Workshop "Challenges in the Dark Sector: Alternatives to the WIMP paradigm”

    CERN Document Server

    2015-01-01

    Identifying what Dark Matter (DM) is, as well as its nature and properties, remains a major challenge for both theoretical and experimental astroparticle physics communities. In the past decades, WIMP DM has been the most hunted candidate, with the result that nowadays WIMPS are cornered by large amounts of experimental data from Direct Detection, Indirect Detection, and Collider Experiments. If no WIMP signal is detected in the next few years, the possibility that this very appealing theoretical idea is not what Nature has chosen will become even more compelling and will boost theoretical studies and experimental searches for non-WIMP alternatives for DM. The aim of this 3-day meeting is to convene experts on alternatives to the WIMP paradigm to stimulate informal discussions on different possibilities (dark photons, axion-like particles, Majorons, self-interacting dark sectors, just to mention a few). We plan to have only three or four talks each day, and plenty of time to discuss implications of these DM s...

  5. Secluded WIMPs, Dark QED with Massive Photons, and the Galactic Center Gamma-Ray Excess

    Science.gov (United States)

    Fortes, E. C. F. S.; Pleitez, V.; Stecker, F. W.

    2015-01-01

    We discuss a particular secluded WIMP dark matter model consisting of neutral fermions as the dark matter candidate and a Proca-Wentzel (PW) field as a mediator. In the model that we consider here, dark matter WIMPs interact with standard model (SM) particles only through the PW field of approximately MeV-multi-GeV mass particles. The interactions occur via a U(1)' mediator, V'(sub mu), which couples to the SM by kinetic mixing with U(1) hypercharge bosons, B'(sub mu). One important difference between our model and other such models in the literature is the absence of an extra singlet scalar, so that the parameter with dimension of mass M(sup 2, sub V) is not related to a spontaneous symmetry breaking. This QED based model is also renormalizable. The mass scale of the mediator and the absence of the singlet scalar can lead to interesting astrophysical signatures. The dominant annihilation channels are different from those usually considered in previous work. We show that the GeV energy gamma-ray excess in the galactic center region, as derived from Fermi-LAT Gamma-ray Space Telescope data, can be attributed to such secluded dark matter WIMPs, given parameters of the model that are consistent with both the cosmological dark matter density and the upper limits on WIMP spin-independent elastic scattering. Secluded WIMP models are also consistent with suggested upper limits on a DM contribution to the cosmic-ray antiproton flux.

  6. Search for bosonic super-WIMP interactions with the XENON100 experiment

    Science.gov (United States)

    Aprile, E.; Aalbers, J.; Agostini, F.; Alfonsi, M.; Althueser, L.; Amaro, F. D.; Anthony, M.; Arneodo, F.; Barrow, P.; Baudis, L.; Bauermeister, B.; Benabderrahmane, M. L.; Berger, T.; Breur, P. A.; Brown, A.; Brown, A.; Brown, E.; Bruenner, S.; Bruno, G.; Budnik, R.; Bütikofer, L.; Calvén, J.; Capelli, C.; Cardoso, J. M. R.; Cichon, D.; Coderre, D.; Colijn, A. P.; Conrad, J.; Cussonneau, J. P.; Decowski, M. P.; de Perio, P.; di Gangi, P.; di Giovanni, A.; Diglio, S.; Eurin, G.; Fei, J.; Ferella, A. D.; Fieguth, A.; Fulgione, W.; Gallo Rosso, A.; Galloway, M.; Gao, F.; Garbini, M.; Geis, C.; Goetzke, L. W.; Greene, Z.; Grignon, C.; Hasterok, C.; Hogenbirk, E.; Howlett, J.; Itay, R.; Kaminsky, B.; Kazama, S.; Kessler, G.; Kish, A.; Landsman, H.; Lang, R. F.; Lellouch, D.; Levinson, L.; Lin, Q.; Lindemann, S.; Lindner, M.; Lombardi, F.; Lopes, J. A. M.; Manfredini, A.; Maris, I.; Marrodán Undagoitia, T.; Masbou, J.; Massoli, F. V.; Masson, D.; Mayani, D.; Messina, M.; Micheneau, K.; Molinario, A.; Morâ, K.; Murra, M.; Naganoma, J.; Ni, K.; Oberlack, U.; Pakarha, P.; Pelssers, B.; Persiani, R.; Piastra, F.; Pienaar, J.; Pizzella, V.; Piro, M.-C.; Plante, G.; Priel, N.; Ramírez García, D.; Rauch, L.; Reichard, S.; Reuter, C.; Rizzo, A.; Rupp, N.; Dos Santos, J. M. F.; Sartorelli, G.; Scheibelhut, M.; Schindler, S.; Schreiner, J.; Schumann, M.; Scotto Lavina, L.; Selvi, M.; Shagin, P.; Silva, M.; Simgen, H.; Sivers, M. V.; Stein, A.; Thers, D.; Tiseni, A.; Trinchero, G.; Tunnell, C.; Vargas, M.; Wang, H.; Wang, Z.; Wei, Y.; Weinheimer, C.; Wittweg, C.; Wulf, J.; Ye, J.; Zhang, Y.; Zhu, T.; Xenon Collaboration

    2017-12-01

    We present results of searches for vector and pseudoscalar bosonic super-weakly interacting massive particles (WIMPs), which are dark matter candidates with masses at the keV-scale, with the XENON100 experiment. XENON100 is a dual-phase xenon time projection chamber operated at the Laboratori Nazionali del Gran Sasso. A profile likelihood analysis of data with an exposure of 224.6 live days ×34 kg showed no evidence for a signal above the expected background. We thus obtain new and stringent upper limits in the (8 - 125 ) keV /c2 mass range, excluding couplings to electrons with coupling constants of ga e>3 ×10-13 for pseudo-scalar and α'/α >2 ×10-28 for vector super-WIMPs, respectively. These limits are derived under the assumption that super-WIMPs constitute all of the dark matter in our galaxy.

  7. Limits on spin-dependent WIMP-nucleon cross sections from the XENON10 experiment.

    Science.gov (United States)

    Angle, J; Aprile, E; Arneodo, F; Baudis, L; Bernstein, A; Bolozdynya, A; Coelho, L C C; Dahl, C E; DeViveiros, L; Ferella, A D; Fernandes, L M P; Fiorucci, S; Gaitskell, R J; Giboni, K L; Gomez, R; Hasty, R; Kastens, L; Kwong, J; Lopes, J A M; Madden, N; Manalaysay, A; Manzur, A; McKinsey, D N; Monzani, M E; Ni, K; Oberlack, U; Orboeck, J; Plante, G; Santorelli, R; dos Santos, J M F; Shagin, P; Shutt, T; Sorensen, P; Schulte, S; Winant, C; Yamashita, M

    2008-08-29

    XENON10 is an experiment to directly detect weakly interacting massive particles (WIMPs), which may comprise the bulk of the nonbaryonic dark matter in our Universe. We report new results for spin-dependent WIMP-nucleon interactions with 129Xe and 131Xe from 58.6 live days of operation at the Laboratori Nazionali del Gran Sasso. Based on the nonobservation of a WIMP signal in 5.4 kg of fiducial liquid xenon mass, we exclude previously unexplored regions in the theoretically allowed parameter space for neutralinos. We also exclude a heavy Majorana neutrino with a mass in the range of approximately 10 GeV/c2-2 TeV/c2 as a dark matter candidate under standard assumptions for its density and distribution in the galactic halo.

  8. Deformed shell model study of event rates for WIMP-73Ge scattering

    Science.gov (United States)

    Sahu, R.; Kota, V. K. B.

    2017-12-01

    The event detection rates for the Weakly Interacting Massive Particles (WIMP) (a dark matter candidate) are calculated with 73Ge as the detector. The calculations are performed within the deformed shell model (DSM) based on Hartree-Fock states. First, the energy levels and magnetic moment for the ground state and two low-lying positive parity states for this nucleus are calculated and compared with experiment. The agreement is quite satisfactory. Then the nuclear wave functions are used to investigate the elastic and inelastic scattering of WIMP from 73Ge; inelastic scattering, especially for the 9/2+ → 5/2+ transition, is studied for the first time. The nuclear structure factors which are independent of supersymmetric model are also calculated as a function of WIMP mass. The event rates are calculated for a given set of nucleonic current parameters. The calculation shows that 73Ge is a good detector for detecting dark matter.

  9. Beyond WIMP: From Theory to Detection of Sub-GeV Dark Matter

    CERN Multimedia

    CERN. Geneva

    2014-01-01

    The existence of dark matter has been well established with overwhelming evidence, but its particle identity is still unknown. For more than three decades, significant theoretical and experimental efforts have been directed towards the search for a Weakly Interacting Massive Particle (WIMP), often overlooking other possibilities. The lack of an unambiguous positive WIMP signal, at both indirect- and direct-detection experiments and at the LHC, stresses the need to expand dark matter research into additional theoretical scenarios and, more importantly, to develop new experimental capabilities that go beyond the limitations of WIMP detection. In this talk I will discuss new theoretical ideas and experimental avenues for searching for light, sub-GeV dark matter. Some emphasis will be given to direct detection experiments, where several new strategies to directly detect dark matter particles with MeV to GeV mass, far below standard direct detection capabilities, are developed.

  10. 750 GeV dark pion: Cousin of a dark G -parity odd WIMP

    Science.gov (United States)

    Bai, Yang; Berger, Joshua; Lu, Ran

    2016-04-01

    We point out a potential common origin of the recently observed 750 GeV diphoton resonance and a weakly interacting massive particle (WIMP) candidate. In a dark QCD sector with an unbroken dark G parity, the diphoton resonance could be a dark G -even pion, while the WIMP could be the lightest dark G -odd pion. Both particles are Standard Model gauge singlets and have the same decay constant. For the dark pion decay constant of around 500 GeV, both the diphoton excess at the LHC and the dark matter thermal abundance can be accommodated in our model. Our model predicts additional dark G -even and dark G -odd color-octet pions within reach of the 13 TeV LHC runs. For the 5 +5 ¯ model, compatible with the grand unified theories, the WIMP mass is predicted to be within (613,750) GeV.

  11. Raman spectroscopy differentiates between sensitive and resistant multiple myeloma cell lines

    Science.gov (United States)

    Franco, Domenico; Trusso, Sebastiano; Fazio, Enza; Allegra, Alessandro; Musolino, Caterina; Speciale, Antonio; Cimino, Francesco; Saija, Antonella; Neri, Fortunato; Nicolò, Marco S.; Guglielmino, Salvatore P. P.

    2017-12-01

    Current methods for identifying neoplastic cells and discerning them from their normal counterparts are often nonspecific and biologically perturbing. Here, we show that single-cell micro-Raman spectroscopy can be used to discriminate between resistant and sensitive multiple myeloma cell lines based on their highly reproducible biomolecular spectral signatures. In order to demonstrate robustness of the proposed approach, we used two different cell lines of multiple myeloma, namely MM.1S and U266B1, and their counterparts MM.1R and U266/BTZ-R subtypes, resistant to dexamethasone and bortezomib, respectively. Then, micro-Raman spectroscopy provides an easily accurate and noninvasive method for cancer detection for both research and clinical environments. Characteristic peaks, mostly due to different DNA/RNA ratio, nucleic acids, lipids and protein concentrations, allow for discerning the sensitive and resistant subtypes. We also explored principal component analysis (PCA) for resistant cell identification and classification. Sensitive and resistant cells form distinct clusters that can be defined using just two principal components. The identification of drug-resistant cells by confocal micro-Raman spectroscopy is thus proposed as a clinical tool to assess the development of resistance to glucocorticoids and proteasome inhibitors in myeloma cells.

  12. Differential sensitivities of bladder cancer cell lines to resveratol are unrelated to its metabolic profile

    Science.gov (United States)

    Li, Hong; Wu, Moli; Ren, Changle; Zhen, Yuhong; Ma, Xiaochi; Diao, Yunpeng; Ma, Xiaodong; Deng, Sa; Liu, Jia

    2017-01-01

    Resveratrol (RV) is a natural polyphenol compound with a wide range of activities, including inhibition of human bladder cancer (HBC) cell growth. Because RV is rapidly metabolized and has poor bioavailability, it is unclear whether the antitumor activity is due to RV or its metabolites. We therefore used liquid chromatography-mass spectroscopy, qRT-PCR, immunocytochemistry and western blotting to evaluate the metabolic profile and biotransformation of RV in the T24 and EJ HBC cell lines. Both T24 and EJ cells generated the same RV metabolite, RV monosulfate (RVS), and both exhibited upregulation of the RV-associated metabolic enzyme SULT1A1 (sulfotransferase). Despite these similarities, T24 cells were more sensitive to RV than EJ cells, yet T24 cells exhibited no sensitivity to an RVS mixture (84.13% RVS). Primary rat bladder epithelial cells showed no adverse effects when exposed to a therapeutic dose (100 μM) of RV. The differences in RV sensitivity between the two HBC cell lines did not reflect differences in the RV metabolic profile or SULT1A1 expression. Because RV exhibited stronger antitumor activity and better safety than RVS, we conclude that RV has significant therapeutic potential for HBC treatment, provided individual differences are considered during clinical research and application. PMID:28178690

  13. Sensitivity amplification of fiber-optic in-line Mach-Zehnder Interferometer sensors with modified Vernier-effect.

    Science.gov (United States)

    Liao, Hao; Lu, Ping; Fu, Xin; Jiang, Xinyue; Ni, Wenjun; Liu, Deming; Zhang, Jiangshan

    2017-10-30

    In this paper, a novel sensitivity amplification method for fiber-optic in-line Mach-Zehnder interferometer (MZI) sensors has been proposed and demonstrated. The sensitivity magnification is achieved through a modified Vernier-effect. Two cascaded in-line MZIs based on offset splicing of single mode fiber (SMF) have been used to verify the effect of sensitivity amplification. Vernier-effect is generated due to the small free spectral range (FSR) difference between the cascaded in-line MZIs. Frequency component corresponding to the envelope of the superimposed spectrum is extracted to take Inverse Fast Fourier Transform (IFFT). Thus we can obtain the envelope precisely from the messy superimposed spectrum. Experimental results show that a maximum sensitivity amplification factor of nearly 9 is realized. The proposed sensitivity amplification method is universal for the vast majority of in-line MZIs.

  14. High-sensitivity supercontinuum-based parallel line-field optical coherence tomography with 1 million A-lines/s (Conference Presentation)

    Science.gov (United States)

    Barrick, Jessica; Doblas, Ana; Sears, Patrick R.; Ostrowski, Lawrence E.; Oldenburg, Amy L.

    2017-02-01

    While traditional, flying-spot, spectral domain OCT systems can achieve MHz linerates, they are limited by the need for mechanical scanning to produce a B-mode image. Line-field OCT (LF OCT) removes the need for mechanical scanning by simultaneously recording all A-lines on a 2D CMOS sensor. Our LF OCT system operates at the highest A-line rate of any spectral domain (SD) LF OCT system reported to date (1,024,000 A-lines/s). This is comparable with the fastest flying-spot SDOCT system reported. Additionally, all OCT systems face a tradeoff between imaging speed and sensitivity. Long exposure times improve sensitivity but can lead to undesirable motion artifacts. LF OCT has the potential to relax this tradeoff between sensitivity and imaging speed because all A-lines are exposed during the entire frame acquisition time. However, this advantage has not yet been realized due to the loss of power-per-A-line by spreading the illumination light across all A-lines on the sample. Here we use a supercontinuum source to illuminate the sample with 500mW of light in the 605-950 nm wavelength band, effectively providing 480 µW of power-per-A-line, with axial and lateral resolutions of 1.8 µm and 14 µm, respectively. With this system we achieve the highest reported sensitivity (113 dB) of any LF OCT system. We then demonstrate the capability of this system by capturing the rapidly beating cilia of human bronchial-epithelial cells in vitro. The combination of high speed and high sensitivity offered by supercontinuum-based LF SD OCT offers new opportunities for studying cell and tissue dynamics.

  15. Stable aneuploid tumors cells are more sensitive to TTK inhibition than chromosomally unstable cell lines.

    Science.gov (United States)

    Libouban, Marion A A; de Roos, Jeroen A D M; Uitdehaag, Joost C M; Willemsen-Seegers, Nicole; Mainardi, Sara; Dylus, Jelle; de Man, Jos; Tops, Bastiaan; Meijerink, Jules P P; Storchová, Zuzana; Buijsman, Rogier C; Medema, René H; Zaman, Guido J R

    2017-06-13

    Inhibition of the spindle assembly checkpoint kinase TTK causes chromosome mis-segregation and tumor cell death. However, high levels of TTK correlate with chromosomal instability (CIN), which can lead to aneuploidy. We show that treatment of tumor cells with the selective small molecule TTK inhibitor NTRC 0066-0 overrides the mitotic checkpoint, irrespective of cell line sensitivity. In stable aneuploid cells NTRC 0066-0 induced acute CIN, whereas in cells with high levels of pre-existing CIN there was only a small additional fraction of cells mis-segregating their chromosomes. In proliferation assays stable aneuploid cells were more sensitive than cell lines with pre-existing CIN. Tetraploids are thought to be an intermediate between diploid and unstable aneuploid cells. TTK inhibitors had the same potency on post-tetraploid and parental diploid cells, which is remarkable because the post-tetraploids are more resistant to mitotic drugs. Finally, we confirm that the reference compound reversine is a TTK inhibitor and like NTRC 0066-0, inhibits the proliferation of patient-derived colorectal cancer organoids. In contrast, treatment with TTK inhibitor did not reduce the viability of non-proliferating T cell acute lymphoblastic leukemia cells samples. Consequently, TTK inhibitor therapy is expected to spare non-dividing cells, and may be used to target stable aneuploid tumors.

  16. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Upper Coast of Texas: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for the Upper...

  17. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Upper Coast of Texas: ESIL (ESI Shoreline Types - Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The ESIL data set contains lines representing the shoreline and coastal habitats of the Upper Coast of Texas, classified according to the Environmental Sensitivity...

  18. Method for Increasing Sensitivity of the Distance Protection on a 330 KV Double-Circuit Transmission Line

    Science.gov (United States)

    Brinkis, K.; Vanzovich, E.; Drozd, D.; Mutule, A.

    2010-01-01

    Possibilities of increasing the distance protection (DP) sensitivity are considered for a double-circuit transmission line connected to a substation busbar only with one circuit-breaker in each phase. The DP sensitivity can be increased on such a line using mutual connections of the phase wires along its whole length. The minimal (optimal) number of the connections is found by the proposed calculation method.

  19. CPN/Tools: A Post-WIMP Interface for Editing and Simulating Coloured Petri Nets

    DEFF Research Database (Denmark)

    Andersen, Peter; Beaudouin-Lafon, Michel; Mackay, Wendy E.

    2001-01-01

    CPN/Tools is a major redesign of the popular Design/CPN tool from the University of Aarhus CPN group. The new interface is based on advanced, post-WIMP interaction techniques, including bi-manual interaction, toolglasses and marking menus and a new metaphor for managing the workspace. It challeng...

  20. Ultra-cold WIMPs relics of non-standard pre-BBN cosmologies

    CERN Document Server

    Gelmini, Graciela B

    2008-01-01

    We point out that in scenarios in which the Universe evolves in a non-standard manner during and after the kinetic decoupling of weakly interacting massive particles (WIMPs), these relics can be much colder than in standard cosmological scenarios (i.e. can be ultra-cold), possibly leading to the formation of smaller first objects in hierarchical structure formation scenarios.

  1. Impacts of WIMP dark matter upon stellar evolution: main-sequence stars

    CERN Document Server

    Scott, Pat; Edsjo, Joakim

    2008-01-01

    The presence of large amounts of WIMP dark matter in stellar cores has been shown to have significant effects upon models of stellar evolution. We present a series of detailed grids of WIMP-influenced stellar models for main sequence stars, computed using the DarkStars code. We describe the changes in stellar structure and main sequence evolution which occur for masses ranging from 0.3 to 2.0 solar masses and metallicities from Z = 0.0003-0.02, as a function of the rate of energy injection by WIMPs. We then go on to show what rates of energy injection can be obtained using realistic orbital parameters for stars near supermassive black holes, including detailed considerations of dark matter halo velocity and density profiles. Capture and annihilation rates are strongly boosted when stars follow elliptical rather than circular orbits, causing WIMP annihilation to provide up to 100 times the energy of hydrogen fusion in stars at the Galactic centre.

  2. Detector Simulation and WIMP Search Analysis for the Cryogenic Dark Matter Search Experiment

    Energy Technology Data Exchange (ETDEWEB)

    McCarthy, Kevin [Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

    2013-06-01

    Astrophysical and cosmological measurements on the scales of galaxies, galaxy clusters, and the universe indicate that 85% of the matter in the universe is composed of dark matter, made up of non-baryonic particles that interact with cross-sections on the weak scale or lower. Hypothetical Weakly Interacting Massive Particles, or WIMPs, represent a potential solution to the dark matter problem, and naturally arise in certain Standard Model extensions. The Cryogenic Dark Matter Search (CDMS) collaboration aims to detect the scattering of WIMP particles from nuclei in terrestrial detectors. Germanium and silicon particle detectors are deployed in the Soudan Underground Laboratory in Minnesota. These detectors are instrumented with phonon and ionization sensors, which allows for discrimination against electromagnetic backgrounds, which strike the detector at rates orders of magnitude higher than the expected WIMP signal. This dissertation presents the development of numerical models of the physics of the CDMS detectors, implemented in a computational package collectively known as the CDMS Detector Monte Carlo (DMC). After substantial validation of the models against data, the DMC is used to investigate potential backgrounds to the next iteration of the CDMS experiment, known as SuperCDMS. Finally, an investigation of using the DMC in a reverse Monte Carlo analysis of WIMP search data is presented.

  3. Multiple mechanisms account for variation in base-line sensitivity to azole fungicides in field isolates of Mycosphaerella graminicola

    NARCIS (Netherlands)

    Stergiopoulos, I.; Nistelrooy, van J.G.M.; Kema, G.H.J.; Waard, de M.A.

    2003-01-01

    Molecular mechanisms that account for variation in base-line sensitivity to azole fungicides were examined in a collection of twenty field isolates, collected in France and Germany, of the wheat pathogen Mycosphaerella graminicola (Fuckel) Schroeter. The isolates tested represent the wide base-line

  4. Inhibition of geranylgeranylation mediates sensitivity to CHOP-induced cell death of DLBCL cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Ageberg, Malin, E-mail: Malin.Ageberg@med.lu.se [Division of Hematology and Transfusion Medicine, Lund University, BMC C14, 221 84 Lund (Sweden); Rydstroem, Karin, E-mail: Karin.Rydstom@skane.se [Department of Oncology, Skanes University Hospital, Allmaenmott, Onkologiska kliniken i Lund, 221 85 Lund (Sweden); Linden, Ola, E-mail: Ola.Linden@skane.se [Department of Oncology, Skanes University Hospital, Allmaenmott, Onkologiska kliniken i Lund, 221 85 Lund (Sweden); Linderoth, Johan, E-mail: Johan.Linderoth@skane.se [Department of Oncology, Skanes University Hospital, Allmaenmott, Onkologiska kliniken i Lund, 221 85 Lund (Sweden); Jerkeman, Mats, E-mail: Mats.Jerkeman@skane.se [Department of Oncology, Skanes University Hospital, Allmaenmott, Onkologiska kliniken i Lund, 221 85 Lund (Sweden); Drott, Kristina, E-mail: Kristina.Drott@med.lu.se [Division of Hematology and Transfusion Medicine, Lund University, BMC C14, 221 84 Lund (Sweden)

    2011-05-01

    Prenylation is a post-translational hydrophobic modification of proteins, important for their membrane localization and biological function. The use of inhibitors of prenylation has proven to be a useful tool in the activation of apoptotic pathways in tumor cell lines. Rab geranylgeranyl transferase (Rab GGT) is responsible for the prenylation of the Rab family. Overexpression of Rab GGTbeta has been identified in CHOP refractory diffuse large B cell lymphoma (DLBCL). Using a cell line-based model for CHOP resistant DLBCL, we show that treatment with simvastatin, which inhibits protein farnesylation and geranylgeranylation, sensitizes DLBCL cells to cytotoxic treatment. Treatment with the farnesyl transferase inhibitor FTI-277 or the geranylgeranyl transferase I inhibitor GGTI-298 indicates that the reduction in cell viability was restricted to inhibition of geranylgeranylation. In addition, treatment with BMS1, a combined inhibitor of farnesyl transferase and Rab GGT, resulted in a high cytostatic effect in WSU-NHL cells, demonstrated by reduced cell viability and decreased proliferation. Co-treatment of BMS1 or GGTI-298 with CHOP showed synergistic effects with regard to markers of apoptosis. We propose that inhibition of protein geranylgeranylation together with conventional cytostatic therapy is a potential novel strategy for treating patients with CHOP refractory DLBCL.

  5. Proteomics study of changes in soybean lines resistant and sensitive to Phytophthora sojae.

    Science.gov (United States)

    Zhang, YuMei; Zhao, JinMing; Xiang, Yang; Bian, XiaoChun; Zuo, QiaoMei; Shen, Qi; Gai, JunYi; Xing, Han

    2011-09-07

    Phytophthora sojae causes soybean root and stem rot, resulting in an annual loss of 1-2 billion US dollars in soybean production worldwide. A proteomic technique was used to determine the effects on soybean hypocotyls of infection with P. sojae. In the present study, 46 differentially expressed proteins were identified in soybean hypocotyls infected with P. sojae, using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization tandem time of flight (MALDI-TOF/TOF). The expression levels of 26 proteins were significantly affected at various time points in the tolerant soybean line, Yudou25, (12 up-regulated and 14 down-regulated). In contrast, in the sensitive soybean line, NG6255, only 20 proteins were significantly affected (11 up-regulated and 9 down-regulated). Among these proteins, 26% were related to energy regulation, 15% to protein destination and storage, 11% to defense against disease, 11% to metabolism, 9% to protein synthesis, 4% to secondary metabolism, and 24% were of unknown function. Our study provides important information on the use of proteomic methods for studying protein regulation during plant-oomycete interactions.

  6. A position- and time-sensitive photon-counting detector with delay- line read-out

    Science.gov (United States)

    Jagutzki, Ottmar; Dangendorf, Volker; Lauck, Ronald; Czasch, Achim; Milnes, James

    2007-05-01

    We have developed image intensifier tubes with delay-anode read-out for time- and position-sensitive photon counting. The timing precision is better than 1 ns with 1000x1000 pixels position resolution and up to one megacounts/s processing rate. Large format detectors of 40 and 75 mm active diameter with internal helical-wire delay-line anodes have been produced and specified. A different type of 40 and 25 mm tubes with semi-conducting screen for image charge read-out allow for an economic and robust tube design and for placing the read-out anodes outside the sealed housing. Two types of external delay-line anodes, i.e. pick-up electrodes for the image charge, have been tested. We present tests of the detector and anode performance. Due to the low background this technique is well suited for applications with very low light intensity and especially if a precise time tagging for each photon is required. As an example we present the application of scintillator read-out in time-of-flight (TOF) neutron radiography. Further applications so far are Fluorescence Life-time Microscopy (FLIM) and Astronomy.

  7. Ultra-sensitive probe of spectral line structure and detection of isotopic oxygen

    Science.gov (United States)

    Garner, Richard M.; Dharamsi, A. N.; Khan, M. Amir

    2018-01-01

    We discuss a new method of investigating and obtaining quantitative behavior of higher harmonic (> 2f) wavelength modulation spectroscopy (WMS) based on the signal structure. It is shown that the spectral structure of higher harmonic WMS signals, quantified by the number of zero crossings and turnings points, can have increased sensitivity to ambient conditions or line-broadening effects from changes in temperature, pressure, or optical depth. The structure of WMS signals, characterized by combinations of signal magnitude and spectral locations of turning points and zero crossings, provides a unique scale that quantifies lineshape parameters and, thus, useful in optimization of measurements obtained from multi-harmonic WMS signals. We demonstrate this by detecting weaker rotational-vibrational transitions of isotopic atmospheric oxygen (16O18O) in the near-infrared region where higher harmonic WMS signals are more sensitive contrary to their signal-to-noise ratio considerations. The proposed approach based on spectral structure provides the ability to investigate and quantify signals not only at linecenter but also in the wing region of the absorption profile. This formulation is particularly useful in tunable diode laser spectroscopy and ultra-precision laser-based sensors where absorption signal profile carries information of quantities of interest, e.g., concentration, velocity, or gas collision dynamics, etc.

  8. The radiation-sensitizing effect of flavopiridol in the esophageal cancer cell line Eca109.

    Science.gov (United States)

    Yao, Yuan; Shi, Jingbin; Zhang, Zhuo; Zhang, Feng; Ma, Ruilan; Zhao, Yan

    2013-06-01

    Flavopiridol is a cyclin-dependent kinase inhibitor. It has shown an antitumor effect against several cancers. In the present study, the radiation-sensitizing effect of flavopiridol was investigated in an esophageal squamous carcinoma cell line, Eca109. The growth inhibitory rate of Eca109 with flavopiridol was determined using the MTT and the radio-sensitizing rate using clonogenic survival assays. The cell cycle distribution and the rate of apoptosis were measured using flow cytometry. The proteins cyclin D1, ERK/pERK, caspase-3, Bax and Bcl-2 were detected using western blot analysis to elucidate the mechanism of the radiosensitization effect. MTT assay showed that flavopiridol inhibited the survival rate of Eca109 cells and the effect was dose-dependent. Its IC50 was 193.3 nmol/l. The result of the clonogenic survival revealed that flavopiridol enhanced the radiosensitivity of Eca109 cells and the sensitization enhancement ratio (SER) was 1.194 at 0.2×IC50. Moreover, we detected that the cells treated with flavorpiridol were arrested at the G2/M phase and the apoptosis caused by radiation was increased. In addition, the proteins caspase-3 and Bax in cells treated with flavopiridol were upregulated, while cyclin D1 and Bcl-2 were downregulated. In conclusion, flavopiridol may enhance the radiosensitivity of Eca109 cells and the radiosensitizing effect of flavopiridol may be mediated by decreasing the levels of the cyclin D1 protein, thus increasing the percentage of cells at G2/M phase.

  9. Aleutian Islands Coastal Resources Inventory and Environmental Sensitivity Maps: ESI (Environmental Sensitivity Index Shoreline Types - Polygons and Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing the shoreline and coastal habitats of the Aleutian Islands classified according to the Environmental...

  10. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Hudson River: ESI (Environmental Sensitivity Index Shoreline Types - Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing the shoreline and coastal habitats for the Hudson River, classified according to the Environmental...

  11. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Northern California: ESI (Environmental Sensitivity Index Shoreline Types - Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing the shoreline and coastal habitats of Northern California, classified according to the Environmental...

  12. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Mississippi: ESI (Environmental Sensitivity Index Shoreline Types - Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing the shoreline and coastal habitats for Mississippi classified according to the Environmental...

  13. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Central California: ESI (Environmental Sensitivity Index Shoreline Types - Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing the shoreline and coastal habitats of Central California classified according to the Environmental...

  14. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: New Hampshire: ESI (Environmental Sensitivity Index Shoreline Types - Polygons and Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing the shoreline and coastal habitats of New Hampshire classified according to the Environmental...

  15. SU-F-T-678: Clotrimazole Sensitizes MCF-7 Breast Cancer Cell Line to Radiation

    Energy Technology Data Exchange (ETDEWEB)

    Garcia, L; Tambasco, M [San Diego State University, San Diego, CA (United States)

    2016-06-15

    Purpose: To study the effects of Clotrimazole (CLT) on radiosensitivity of MCF-7 Cells in correlation to detachment of Hexokinase II from the Voltage Dependent Anion Channel on the outer membrane of the mitochondria. Apoptotic fractions were also analyzed in relation to the detachment of Hexokinase. Methods: This study focused on the mammary adenocarcinoma cell line, MCF-7. Colony forming assays were used to analyze radiosensitization by CLT. Flow cytometry methods were used to analyze apoptotic vs necrotic fractions after treatment with CLT. Spectrophotometery was used to analyze the mitochondrial bound and soluble fraction of Hexokinase by means of relative enzymatic activity. Results: Our preliminary data have shown that CLT sensitizes MCF-7 cells to radiation in a dose and incubation time dependent manner up. We have also demonstrated that there are two radiosensitizing periods in MCF-7 cells with the first corresponding to the cycle arrest after 24 hours observed in other cell lines. The second radiosensitizing period occurs with incubation in CLT after irradiation which reaches maximum effect around 24 hours of incubation time. Preliminary data from our Hexokinase detachment assay show a factor of two increase in the ratio of unbound to bound Hexokinase when comparing incubation for 24 hours in media containing 0 and 20 µM CLT. Conclusion: This study and others indicate CLT as a possible radiosensitizing agent in cancer therapies. While CLT itself shows toxicity to the liver in high doses, this study further demonstrates that disruption of the Warburg Effect and unbinding of mitochondrial bound Hexokinase as a possible pathway for cancer treatment.

  16. A Study of Nuclear Recoils in Liquid Argon Time Projection Chamber for the Direct Detection of WIMP Dark Matter

    Energy Technology Data Exchange (ETDEWEB)

    Cao, Huajie [Princeton Univ., NJ (United States)

    2014-11-01

    Robust results of WIMP direct detection experiments depend on rm understandings of nuclear recoils in the detector media. This thesis documents the most comprehensive study to date on nuclear recoils in liquid argon - a strong candidate for the next generation multi-ton scale WIMP detectors. This study investigates both the energy partition from nuclear recoil energy to secondary modes (scintillation and ionization) and the pulse shape characteristics of scintillation from nuclear recoils.

  17. Limits on Light WIMPs with a Germanium Detector at 172 eVee threshold at the China Jinping Underground Laboratory

    CERN Document Server

    Liu, S K; Kang, K J; Cheng, J P; Wong, H T; Li, Y J; Lin, S T; Chang, J P; Chen, N; Chen, Q H; Chen, Y H; Chuang, Y C; Deng, Z; Du, Q; Gong, H; Hao, X Q; He, H J; He, Q J; Huang, H X; Huang, T R; Jiang, H; Li, H B; Li, J M; Li, J; Li, X; Li, X Q; Li, X Y; Li, Y L; Liao, H Y; Lin, F K; Lü, L C; Ma, H; Mao, S J; Qin, J Q; Ren, J; Ruan, X C; Shen, M B; Singh, L; Singh, M K; Soma, A K; Su, J; Tang, C J; Tseng, C H; Wang, J M; Wang, L; Wang, Q; Wu, S Y; Wu, Y C; Xianyu, Z Z; Xiao, R Q; Xing, H Y; Xu, F Z; Xu, Y; Xu, X J; Xue, T; Yang, C W; Yang, L T; Yang, S W; Yi, N; Yu, C X; Yu, H; Yu, X Z; Zeng, X H; Zeng, Z; Zhang, L; Zhang, Y H; Zhao, M G; Zhao, W; Zhou, Z Y; Zhu, J J; Zhu, W B; Zhu, X Z; Zhu, Z H

    2014-01-01

    The China Dark Matter Experiment reports results on light WIMP dark matter searches at the China Jinping Underground Laboratory with a germanium detector array with a total mass of 20 g. The physics threshold achieved is 172 eVee at 50% signal efficiency. With 0.784 kg-days of data, exclusion region on spin-independent coupling with the nucleon is derived, improving over our earlier bounds at WIMP mass less than 4.6 GeV.

  18. The Diurnal Variation of the Wimp Detection Event Rates in Directional Experiments

    CERN Document Server

    Vergados, J D

    2009-01-01

    The recent WMAP data have confirmed that exotic dark matter together with the vacuum energy (cosmological constant) dominate in the flat Universe. Modern particle theories naturally provide viable cold dark matter candidates with masses in the GeV-TeV region. Supersymmetry provides the lightest supersymmetric particle (LSP), theories in extra dimensions supply the lightest Kaluza-Klein particle (LKP) etc. The nature of dark matter can only be unraveled only by its direct detection in the laboratory. All such candidates will be called WIMPs (Weakly Interacting Massive Particles). In any case the direct dark matter search, which amounts to detecting the recoiling nucleus, following its collision with WIMP, is central to particle physics and cosmology. In this work we briefly review the theoretical elements relevant to the direct dark matter detection experiments, paying particular attention to directional experiments. i.e experiments in which, not only the energy but the direction of the recoiling nucleus is ob...

  19. Effects of extra virgin olive oil phenols on HL60 cell lines sensitive and resistant to anthracyclines

    Directory of Open Access Journals (Sweden)

    M. Crescimanno

    2009-01-01

    Full Text Available The aim of our study was to evaluate the capability of a crude extract of phenols from extra virgin olive oil of Moraiolo cultivar to induce apoptosis and/or differentiation in sensitive and resistant HL60 cell lines to anticancer drugs (Typical Multidrug Resistance. Our data highlight that the crude extract is able to induce apoptosis on both sensitive and resistant cells, whereas the exposure to a number of anticancer drugs does not induce apoptosis in resistant cells. In differentiation experiments we investigated the capability of crude extract of phenols to induce the expression of CD11 granulocytic or CD14 monocytic cell surface antigen in sensitive and resistant HL60 cell lines. At IC50 dose level (17 ug/ml and 32 ug/ml respectively for sensitive and resistant cell lines, the crude extract induced differentiation associated with the expression of CD14 monocytic cell lines but not that of CD11 granulocityc cell surface antigen. Further investigations are in progress to better clarify the mechanism by which olive oil phenols induce diffentiation on this cell line.

  20. Reevaluation of spin-dependent WIMP-proton interactions as an explanation of the DAMA data

    Science.gov (United States)

    Del Nobile, Eugenio; Gelmini, Graciela B.; Georgescu, Andreea; Huh, Ji-Haeng

    2015-08-01

    We reexamine the interpretation of the annual modulation signal observed by the DAMA experiment as due to WIMPs with a spin-dependent coupling mostly to protons. We consider both axial-vector and pseudo-scalar couplings, and elastic as well as endothermic and exothermic inelastic scattering. We conclude that the DAMA signal is in strong tension with null results of other direct detection experiments, particularly PICASSO and KIMS.

  1. Reevaluation of spin-dependent WIMP-proton interactions as an explanation of the DAMA data

    Energy Technology Data Exchange (ETDEWEB)

    Nobile, Eugenio Del; Gelmini, Graciela B.; Georgescu, Andreea; Huh, Ji-Haeng [Department of Physics and Astronomy, UCLA,475 Portola Plaza, Los Angeles, CA 90095 (United States)

    2015-08-25

    We reexamine the interpretation of the annual modulation signal observed by the DAMA experiment as due to WIMPs with a spin-dependent coupling mostly to protons. We consider both axial-vector and pseudo-scalar couplings, and elastic as well as endothermic and exothermic inelastic scattering. We conclude that the DAMA signal is in strong tension with null results of other direct detection experiments, particularly PICASSO and KIMS.

  2. Influence of the HER receptor ligand system on sensitivity to cetuximab and trastuzumab in gastric cancer cell lines.

    Science.gov (United States)

    Kneissl, Julia; Hartmann, Anja; Pfarr, Nicole; Erlmeier, Franziska; Lorber, Thomas; Keller, Simone; Zwingenberger, Gwen; Weichert, Wilko; Luber, Birgit

    2017-04-01

    Gastric cancer remains a major health concern, and improvement of the therapeutic options is crucial. Treatment with targeted therapeutics such as the EGFR-targeting antibody cetuximab or the HER2-targeting antibody trastuzumab is either ineffective or moderately effective in this disease, respectively. In this study, we analysed the involvement of the HER receptor ligands amphiregulin (AREG), epidermal growth factor (EGF), heparin-binding epidermal growth factor (HB-EGF) and transforming growth factor alpha (TGFα) in the responsiveness of gastric cancer cell lines to cetuximab and trastuzumab. A panel of 11 gastric cancer cell lines was characterized for cetuximab and trastuzumab sensitivity, ligand secretion and expression and activation of the HER receptors using WST-1 cell proliferation assays, ELISAs and Western blot analyses. We further investigated the effects of an exogenous ligand application on the cetuximab and trastuzumab sensitivity. We found no correlation between TGFα secretion and the sensitivity to cetuximab or trastuzumab. For AREG, we confirmed previous results indicating that this ligand is a positive predictor of cetuximab sensitivity. Exogenous HB-EGF was effective in rescuing sensitive cell lines from inhibition of cell proliferation by both, cetuximab and trastuzumab. Our data indicate that HB-EGF may be a useful marker for the prediction of trastuzumab sensitivity in gastric cancer.

  3. Velocity- and acceleration-sensitive units in the trunk lateral line of the trout

    NARCIS (Netherlands)

    Kroese, A. B.; Schellart, N. A.

    1992-01-01

    1. The two main types of lateral line organs of lower vertebrates are the superficial neuromasts (SN), with a cupula that protrudes in the surrounding water, and the canal neuromasts (CN), located in the lateral line canal. The scales of the trunk lateral line canal of fish contain SNs as well as

  4. Predictive performance for human skin sensitizing potential of the human cell line activation test (h-CLAT).

    Science.gov (United States)

    Nukada, Yuko; Ashikaga, Takao; Sakaguchi, Hitoshi; Sono, Sakiko; Mugita, Nanae; Hirota, Morihiko; Miyazawa, Masaaki; Ito, Yuichi; Sasa, Hitoshi; Nishiyama, Naohiro

    2011-12-01

    Recent changes in regulatory restrictions and social opposition to animal toxicology experiments have driven the need for reliable in vitro tests for predicting the skin sensitizing potentials of a wide variety of industrial chemicals. Previously, we developed the human cell line activation test (h-CLAT) as a cell-based assay to predict the skin sensitizing potential of chemicals, and showed the correspondence between the h-CLAT and the murine local lymph node assay results. This study was conducted to investigate the predictive performance of the h-CLAT for human skin sensitizing potential. We selected a total of 66 test chemicals with known human sensitizing potential, and tested all chemicals with the h-CLAT. We then evaluated the performance of the h-CLAT in predicting human sensitizing potential. Forty-five of 51 tested sensitizers were positive in the h-CLAT, indicating relatively high sensitivity. Also, 10 of 15 non-sensitizers were correctly detected as negative. The overall agreement between human data and h-CLAT outcome was 83%. Furthermore, the h-CLAT could accurately predict the human sensitizing potential of 23 tested chemicals that were amines, heterocyclic compounds, or sulfur compounds. Our data indicate the utility of the h-CLAT for predicting the human skin sensitizing potential of a variety of chemicals. © 2011 John Wiley & Sons A/S.

  5. Comparison of radiation sensitivity for three cell lines as measured by the cloning assay and the micro-nucleus test

    NARCIS (Netherlands)

    Stap, J.; Aten, J. A.

    1990-01-01

    The correlation between cell killing and the induction of micro-nuclei was studied for three cell lines after treatment with gamma radiation to investigate whether the frequency of micro-nucleated cells can be used to determine the radiation sensitivity of a cell type. R1 rat rhabdomyosarcoma cells

  6. [Effects of RNA interference silencing RhoC gene upon paclitaxel sensitivity in ovarian cancer cell lines].

    Science.gov (United States)

    Pan, Ying; Zhang, Wen-ying; Zou, Ji-yan; Sheng, Min-jia; Xuan, Li-li; Hai, De-yang

    2009-06-02

    To explore the changes of paclitaxel sensitivity by RNA interference of RhoC gene in ovarian cancer cell lines. The microRNA (miRNA) targeting RhoC gene was designed and synthesized by in vitro transcription and transfected into ovarian cancer cell line SKOV3. The mRNA and protein of RhoC were detected by the means of RT-PCR and Western blot. The changes of paclitaxel sensitivity after interference were examined by methyl thiazolyl tetrazolium (MTf) assay. At the same time, the mRNA of apoptosis gene Caspase-3 and survivin were evaluated by RT-PCR. In SKOV3 cell, the mRNA and protein of RhoC dramatically decreased at 24, 48 and 72 hours post-transfection. The sensitivity of SKOV3 cell line to paclitaxel increased significantly after turning off the RhoC gene. The proapoptotic gene Caspase-3 increased significantly and anti-apoptosis gene survivin dramatically decreased after the combination treatment of paclitaxel and RhoC-miRNA in comparison with paclitaxel alone treatment. The sensitivity of ovarian cancer cell lines SKOV3 to paclitaxel can be enhanced by RNA interfering RhoC gene. The synergistic mechanism is possibly correlated with the up-regulation of Caspase-3 gene and the down-regulation of survivin gene.

  7. Prospects for SIMPLE 2000 A large-mass, low-background Superheated Droplet Detector for WIMP searches

    CERN Document Server

    Collar, J I; Girard, T A; Limagne, D; Miley, H S; Waysand, G

    2000-01-01

    SIMPLE 2000 ({\\underline S}uperheated {\\underline I}nstrument for {\\underline M}assive {\\underline P}artic{\\underline {LE}} searches) will consist of an array of eight to sixteen large active mass ($\\sim15$ g) Superheated Droplet Detectors(SDDs) to be installed in the new underground laboratory of Rustrel-Pays d'Apt. Several factors make of SDDs an attractive approach for the detection of Weakly Interacting Massive Particles (WIMPs), namely their intrinsic insensitivity to minimum ionizing particles, high fluorine content, low cost and operation near ambient pressure and temperature. We comment here on the fabrication, calibration and already-competitive first limits from SIMPLE prototype SDDs, as well as on the expected immediate increase in sensitivity of the program, which aims at an exposure of $>$25 kg-day during the year 2000. The ability of modest-mass fluorine-rich detectors to explore regions of neutralino parameter space beyond the reach of the most ambitious cryogenic projects is pointed out.

  8. Sensitivity of malignant rhabdoid tumor cell lines to PD 0332991 is inversely correlated with p16 expression

    Energy Technology Data Exchange (ETDEWEB)

    Katsumi, Yoshiki; Iehara, Tomoko; Miyachi, Mitsuru; Yagyu, Shigeki; Tsubai-Shimizu, Satoko; Kikuchi, Ken; Tamura, Shinichi; Kuwahara, Yasumichi; Tsuchiya, Kunihiko; Kuroda, Hiroshi; Sugimoto, Tohru [Department of Pediatrics, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kawaramachi-hirokoji, Kamigyo-ku, Kyoto 602-8566 (Japan); Houghton, Peter J. [Molecular Pharmacology, St. Jude Children' s Research Hospital, 332 N. Lauderdale St. Memphis, TN 38105-2794 (United States); Hosoi, Hajime, E-mail: hhosoi@koto.kpu-m.ac.jp [Department of Pediatrics, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kawaramachi-hirokoji, Kamigyo-ku, Kyoto 602-8566 (Japan)

    2011-09-16

    Highlights: {yields} PD 0332991 (PD) could suppress four of five malignant rhabdoid tumor (MRT) cell lines. {yields} The sensitivity of the MRT cell lines to PD was inversely correlated with p16 expression (r = 0.951). {yields} p16 expression in MRT could be used to predict its sensitivity to PD. {yields} PD may be an attractive agent for patients with MRT whose tumors express low levels of p16. -- Abstract: Malignant rhabdoid tumor (MRT) is a rare and highly aggressive neoplasm of young children. MRT is characterized by inactivation of integrase interactor 1 (INI1). Cyclin-dependent kinase 4 (CDK4), which acts downstream of INI1, is required for the proliferation of MRT cells. Here we investigated the effects of PD 0332991 (PD), a potent inhibitor of CDK4, against five human MRT cell lines (MP-MRT-AN, KP-MRT-RY, G401, KP-MRT-NS, KP-MRT-YM). In all of the cell lines except KP-MRT-YM, PD inhibited cell proliferation >50%, (IC{sub 50} values 0.01 to 0.6 {mu}M) by WST-8 assay, and induced G1-phase cell cycle arrest, as shown by flow cytometry and BrdU incorporation assay. The sensitivity of the MRT cell lines to PD was inversely correlated with p16 expression (r = 0.951). KP-MRT-YM cells overexpress p16 and were resistant to the growth inhibitory effect of PD. Small interfering RNA against p16 significantly increased the sensitivity of KP-MRT-YM cells to PD (p < 0.05). These results suggest that p16 expression in MRT could be used to predict its sensitivity to PD. PD may be an attractive agent for patients with MRT whose tumors express low levels of p16.

  9. A Search for WIMP Dark Matter Using the First Five-Tower Run of the Cryogenic Dark Matter Search

    Energy Technology Data Exchange (ETDEWEB)

    Filippini, Jeffrey Peter [UC, Berkeley

    2008-01-01

    In recent decades astronomers and physicists have accumulated a vast array of evidence that the bulk of the universe's matter is in some non-baryonic form that remains undetected by electromagnetic means. This \\dark matter" resides in diuse halos surrounding galaxies and other cosmic structures. Particle theorists have proposed a wide array of candidates for its nature. One particularly promising class of candidates are Weakly Interacting Massive Particles (WIMPs): quanta with masses of order 100 GeV/c2 and interactions characteristic of the weak nuclear force. The Cryogenic Dark Matter Search (CDMS) experiment seeks to directly detect the rare elastic interactions of galactic WIMPs with terrestrial nuclei. To this end, CDMS operates an array of crystalline Ge and Si particle detectors in Soudan Underground Laboratory in northern Minnesota. These crystals are operated at millikelvin temperatures and instrumented to measure the ionization and athermal phonons generated by each particle interaction. This combination provides a powerful two-fold discrimination against the interactions of particles generated by radioactive decay and cosmogenic showers. This dissertation describes the commissioning, analysis, and results of the rst WIMP-search data runs of the CDMS experiment with its full complement of 5 \\Towers" of detectors. These data represent a substantial increase in target mass and exposure over previous CDMS results. The results of this work place the most stringent limits yet set upon the WIMP-nucleon spin-independent cross section for WIMP masses above 44 GeV/c2 , as well as setting competitive limits on spin-dependent WIMP-nucleon interactions. This work also outlines the larger context of this and other probes of the WIMP theory of dark matter, as well as some current development eorts toward a larger cryogenic experiment.

  10. Exclusion limits on the WIMP nucleon elastic scattering cross-section from the Cryogenic Dark Matter Search

    Energy Technology Data Exchange (ETDEWEB)

    Golwala, Sunil Ramanlal [UC, Berkeley

    2000-01-01

    Extensive evidence indicates that a large fraction of the matter in the universe is nonluminous, nonbaryonic, and “cold” — nonrelativistic at the time matter began to dominate the energy density of the universe. Weakly Interacting Massive Particles (WIMPs) are an excellent candidate for nonbaryonic, cold dark matter. Minimal supersymmetry provides a natural WIMP candidate in the form of the lightest superpartner, with a typical mass Mδ ~ 100 GeV c-2 . WIMPs are expected to have collapsed into a roughly isothermal, spherical halo within which the visible portion of our galaxy resides. They would scatter off nuclei via the weak interaction, potentially allowingtheir direct detection. The Cryogenic Dark Matter Search (CDMS) employs Ge and Si detectors to search for WIMPs via their elastic-scatteringinteractions with nuclei while discriminatingagainst interactions of background particles. The former yield nuclear recoils while the latter produce electron recoils. The ionization yield (the ratio of ionization production to recoil energy in a semiconductor) of a particle interaction differs greatly for nuclear and electron recoils. CDMS detectors measure phonon and electron-hole-pair production to determine recoil energy and ionization yield for each event and thereby discriminate nuclear recoils from electron recoils. This dissertation reports new limits on the spin-independent WIMP-nucleon elastic-scattering cross section that exclude unexplored parameter space above 10 GeV c-2 WIMP mass and, at > 75% CL, the entire 3σ allowed region for the WIMP signal reported by the DAMA experiment. The experimental apparatus, detector performance, and data analysis are fully described.

  11. LINES

    Directory of Open Access Journals (Sweden)

    Minas Bakalchev

    2015-10-01

    Full Text Available The perception of elements in a system often creates their interdependence, interconditionality, and suppression. The lines from a basic geometrical element have become the model of a reductive world based on isolation according to certain criteria such as function, structure, and social organization. Their traces are experienced in the contemporary world as fragments or ruins of a system of domination of an assumed hierarchical unity. How can one release oneself from such dependence or determinism? How can the lines become less “systematic” and forms more autonomous, and less reductive? How is a form released from modernistic determinism on the new controversial ground? How can these elements or forms of representation become forms of action in the present complex world? In this paper, the meaning of lines through the ideas of Le Corbusier, Leonidov, Picasso, and Hitchcock is presented. Spatial research was made through a series of examples arising from the projects of the architectural studio “Residential Transformations”, which was a backbone for mapping the possibilities ranging from playfulness to exactness, as tactics of transformation in the different contexts of the contemporary world.

  12. Dye sensitized photovoltaic miniaturized solar cells, used as optical sensors for line of sight detection

    Science.gov (United States)

    Cesar, Cortes Torres Carlos; Sampei, Kota; Miho, Ogawa; Masataka, Ozawa; Norihisa, Miki

    2014-11-01

    Dye sensitized photovoltaic devices have been studied as transparent and low-cost solar cells. Our group have miniaturized the cells and used them as transparent optical sensors. This paper reports the design and fabrication of the cells and avoids the cross talk among cells, which was found recently and such effect provokes hardware instability. We use these optical sensors as an eye tracking device. The sensor array detects the difference in the intensity of light reflected from the pupil and the sclera and then determines the pupil position. Each sensor consists of two electrodes and electrolyte; hence our device conformed by only four semi-circular shaped sensors on eyeglasses can detect the view angle in both horizontal and vertical directions. Manufacturing process gives us freedom to easily re-arrange, add or remove sensors. In our prior work we had good performance in stand-alone configuration. We used specialized equipment from National Instruments for our measurements. However we found that: A cell is not 100% independent from the others, is affected by the absence or presence of light at the neighbour cells. When our device is connected to other electronic devices (for data processing), all cells have the same voltage among them; therefore, all cells behave the same way when any of them is affected by light. The root cause is, due to all sensors were interconnected via a micro channel and filled with electrolyte, due to its conductive properties, electrolyte does neither need electrodes nor physical paths to conduct electricity, so it creates a liquid wire between sensors, hence the gap between them become inexistent, consequently when our device is connected to other electronic devices, due to this unique channel and by sharing a common electronic ground, this connection provokes the voltage to be the same among all sensors in the array. Our device becomes four separate voltage lines in a parallel circuit. The device was also in short circuit provoked

  13. Dark matter sensitivity of multi-ton liquid xenon detectors

    Energy Technology Data Exchange (ETDEWEB)

    Schumann, Marc; Bütikofer, Lukas [Albert Einstein Center for Fundamental Physics, Universität Bern, Sidlerstrasse 5, 3012 Bern (Switzerland); Baudis, Laura; Kish, Alexander [Physik-Institut, Universität Zürich, Winterthurerstrasse 190, 8057 Zürich (Switzerland); Selvi, Marco, E-mail: marc.schumann@lhep.unibe.ch, E-mail: lbaudis@physik.uzh.ch, E-mail: lukas.buetikofer@lhep.unibe.ch, E-mail: alexkish@physik.uzh.ch, E-mail: marco.selvi@bo.infn.it [INFN—Sezione di Bologna, Via Irnerio 46, 40126 Bologna (Italy)

    2015-10-01

    We study the sensitivity of multi ton-scale time projection chambers using a liquid xenon target, e.g., the proposed DARWIN instrument, to spin-independent and spin-dependent WIMP-nucleon scattering interactions. Taking into account realistic backgrounds from the detector itself as well as from neutrinos, we examine the impact of exposure, energy threshold, background rejection efficiency and energy resolution on the dark matter sensitivity. With an exposure of 200 t × y and assuming detector parameters which have been already demonstrated experimentally, spin-independent cross sections as low as 2.5 × 10{sup −49} cm{sup 2} can be probed for WIMP masses around 40 GeV/c{sup 2}. Additional improvements in terms of background rejection and exposure will further increase the sensitivity, while the ultimate WIMP science reach will be limited by neutrinos scattering coherently off the xenon nuclei.

  14. Multiple kinase pathways involved in the different de novo sensitivity of pancreatic cancer cell lines to 17-AAG.

    Science.gov (United States)

    Liu, Heping; Zhang, Ti; Chen, Rong; McConkey, David J; Ward, John F; Curley, Steven A

    2012-07-01

    17-Allylamino-17-demethoxygeldanamycin (17-AAG) specifically targets heat shock protein (HSP)90 and inhibits its chaperoning functions for multiple kinases involved in cancer cell growth and survival. To select responsive patients, the molecular mechanisms underlying the sensitivity of cancer cells to 17-AAG must be elucidated. We used cytotoxicity assays and Western blotting to explore the effects of 17-AAG and sorafenib on cell survival and expression of multiple kinases in the pancreatic cancer cell lines AsPC-1 and Panc-1. Gene cloning and transfection, siRNA silencing, and immunohistochemistry were used to evaluate the effects of mutant p53 protein on 17-AAG sensitivity. AsPC-1 and Panc-1 responded differently to 17-AAG, with half maximal inhibitory concentration (IC(50)) values of 0.12 and 3.18 μM, respectively. Comparable expression of HSP90, HSP70, and HSP27 was induced by 17-AAG in AsPC-1 and Panc-1 cells. P-glycoprotein and mutant p53 did not affect 17-AAG sensitivity in these cell lines. Multiple kinases are more sensitive to HSP90 inhibition in AsPC-1 than in Panc-1 cells. After 17-AAG treatment, p-Bad (S112) decreased in AsPC-1 cells and increased in Panc-1 cells. Sorafenib markedly increased p-Akt, p-ERK1/2, p-GSK-3β, and p-S6 in both cell lines. Accordingly, 17-AAG and sorafenib acted antagonistically in AsPC-1 and Panc-1 cells, except at high concentrations in AsPC-1 cells. Differential inhibition of multiple kinases is responsible for the different de novo sensitivity of AsPC-1 and Panc-1 cells to HSP90 inhibition. P-glycoprotein and mutant p53 protein did not play a role in the sensitivity of pancreatic cancer cells to 17-AAG. Copyright © 2012 Elsevier Inc. All rights reserved.

  15. Polarization-sensitive spectral-domain optical coherence tomography using a single line scan camera

    NARCIS (Netherlands)

    Cense, B.; Mujat, M.; Chen, T.; Park, B. H.; de Boer, J.F.

    2007-01-01

    Polarization-sensitive optical coherence tomography can be used to measure the birefringence of biological tissue such as the human retina. Previous measurements with a time-domain polarization-sensitive optical coherence tomography system revealed that the birefringence of the human retinal nerve

  16. Western Alaska ESI: ESI (Environmental Sensitivity Index Shoreline Types - Polygons and Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing the shoreline and coastal habitats of Western Alaska classified according to the Environmental...

  17. Coastal Resources Atlas: Long Island: ESI (Environmental Sensitivity Index Shoreline Types - Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing the shoreline and coastal habitats of Long Island, New York, classified according to the Environmental...

  18. Columbia River ESI: ESI (Environmental Sensitivity Index Shoreline Types - Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing the shoreline and coastal habitats of Columbia River classified according to the Environmental...

  19. Bristol Bay, Alaska Subarea ESI: ESI (Environmental Sensitivity Index Shoreline Types - Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing the shoreline and coastal habitats of the Bristol Bay Subarea, classified according to the...

  20. Southeast Alaska ESI: ESI (Environmental Sensitivity Index Shoreline Types - Polygons and Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing the shoreline and coastal habitats of Southeast Alaska classified according to the Environmental...

  1. Cook Inlet and Kenai Peninsula, Alaska ESI: ESI (Environmental Sensitivity Index Shoreline Types - Polygons and Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing the shoreline and coastal habitats of Cook Inlet and Kenai Peninsula, Alaska, classified according to...

  2. North Slope, Alaska ESI: ESI (Environmental Sensitivity Index Shoreline Types - Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing the shoreline and coastal habitats for the North Slope of Alaska classified according to the...

  3. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Hudson River: RVRMILES (River Mile Marker Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains human-use resource data for river miles along the Hudson River. Vector lines in this data set represent river mile markers. This data set...

  4. American Samoa ESI: ESI (Environmental Sensitivity Index Shoreline Types - Polygons and Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing the shoreline and coastal habitats of American Samoa classified according to the Environmental...

  5. Aleutian Islands Coastal Resources Inventory and Environmental Sensitivity Maps: FISHL (Fish Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains biological resource data for anadromous fish streams in the Aleutian Islands, Alaska. Vector lines in this data set represent locations of...

  6. A novel line immunoassay based on recombinant virulence factors enables highly specific and sensitive serologic diagnosis of Helicobacter pylori infection.

    Science.gov (United States)

    Formichella, Luca; Romberg, Laura; Bolz, Christian; Vieth, Michael; Geppert, Michael; Göttner, Gereon; Nölting, Christina; Walter, Dirk; Schepp, Wolfgang; Schneider, Arne; Ulm, Kurt; Wolf, Petra; Busch, Dirk H; Soutschek, Erwin; Gerhard, Markus

    2013-11-01

    Helicobacter pylori colonizes half of the world's population, and infection can lead to ulcers, gastric cancer, and mucosa-associated lymphoid tissue (MALT) lymphoma. Serology is the only test applicable for large-scale, population-based screening, but current tests are hampered by a lack of sensitivity and/or specificity. Also, no serologic test allows the differentiation of type I and type II strains, which is important for predicting the clinical outcome. H. pylori virulence factors have been associated with disease, but direct assessment of virulence factors requires invasive methods to obtain gastric biopsy specimens. Our work aimed at the development of a highly sensitive and specific, noninvasive serologic test to detect immune responses to important H. pylori virulence factors. This line immunoassay system (recomLine) is based on recombinant proteins. For this assay, six highly immunogenic virulence factors (CagA, VacA, GroEL, gGT, HcpC, and UreA) were expressed in Escherichia coli, purified, and immobilized to nitrocellulose membranes to detect serological immune responses in patient's sera. For the validation of the line assay, a cohort of 500 patients was screened, of which 290 (58.0%) were H. pylori negative and 210 (42.0%) were positive by histology. The assay showed sensitivity and specificity of 97.6% and 96.2%, respectively, compared to histology. In direct comparison to lysate blotting and enzyme-linked immunosorbent assay (ELISA), the recomLine assay had increased discriminatory power. For the assessment of individual risk for gastrointestinal disease, the test must be validated in a larger and defined patient cohort. Taking the data together, the recomLine assay provides a valuable tool for the diagnosis of H. pylori infection.

  7. A search for WIMPs and tests of local dark matter velocity distributions with the CoGeNT public dataset

    Science.gov (United States)

    Bellis, Matthew; Kelso, Chris; Collar, Juan; Fields, Nicole

    2014-03-01

    Since December 2009, the CoGeNT experiment has recorded interactions in the detector with the goal of either detecting dark matter or setting stringent limits on the mass and cross section of these particles, assuming that dark matter is a form of WIMP (Weakly Interacting Massive Particle). The collaboration has made public this dataset to the broader community and this analysis is based on that dataset. We perform an unbinned, maximum likelihood fit to the data, accounting for known backgrounds and systematic effects. We model the WIMP signal, parametrized by energy deposition and time of year, mass, cross-section, and choice of local WIMP velocity distribution. The velocity distribution is modeled with a Maxwellian-Boltzman distribution, as well as more directional streams. The current status of this analysis will be presented.

  8. Improved phase sensitivity in spectral domain phase microscopy using line-field illumination and self phase-referencing

    Science.gov (United States)

    Yaqoob, Zahid; Choi, Wonshik; Oh, Seungeun; Lue, Niyom; Park, Yongkeun; Fang-Yen, Christopher; Dasari, Ramachandra R.; Badizadegan, Kamran; Feld, Michael S.

    2010-01-01

    We report a quantitative phase microscope based on spectral domain optical coherence tomography and line-field illumination. The line illumination allows self phase-referencing method to reject common-mode phase noise. The quantitative phase microscope also features a separate reference arm, permitting the use of high numerical aperture (NA > 1) microscope objectives for high resolution phase measurement at multiple points along the line of illumination. We demonstrate that the path-length sensitivity of the instrument can be as good as 41 pm/Hz, which makes it suitable for nanometer scale study of cell motility. We present the detection of natural motions of cell surface and two-dimensional surface profiling of a HeLa cell. PMID:19550464

  9. Sensitivity of Malignant Rhabdoid Tumor cell lines to PD 0332991 is inversely correlated with p16 expression

    Science.gov (United States)

    Katsumi, Yoshiki; Iehara, Tomoko; Miyachi, Mitsuru; Yagyu, Shigeki; Tsubai-Shimizu, Satoko; Kikuchi, Ken; Tamura, Shinichi; Kuwahara, Yasumichi; Tsuchiya, Kunihiko; Kuroda, Hiroshi; Sugimoto, Tohru; Houghton, Peter J.; Hosoi, Hajime

    2011-01-01

    Malignant rhabdoid tumor (MRT) is a rare and highly aggressive neoplasm of young children. MRT is characterized by inactivation of integrase interactor 1 (INI1). Cyclin-dependent kinase 4 (CDK4), which acts downstream of INI1, is required for the proliferation of MRT cells. Here we investigated the effects of PD 0332991 (PD), a potent inhibitor of CDK4, against five human MRT cell lines (MP-MRT-AN, KP-MRT-RY, G401, KP-MRT-NS, KP-MRT-YM). In all of the cell lines except KP-MRT-YM, PD inhibited cell proliferation > 50 %, (IC50 values 0.01 to 0.6 µM) by WST-8 assay, and induced G1-phase cell cycle arrest, as shown by flow cytometry and BrdU incorporation assay. The sensitivity of the MRT cell lines to PD was inversely correlated with p16 expression (r = 0.951). KP-MRT-YM cells overexpress p16 and were resistant to the growth inhibitory effect of PD. Small interfering RNA against p16 significantly increased the sensitivity of KP-MRT-YM cells to PD (p MRT could be used to predict its sensitivity to PD. PD may be an attractive agent for patients with MRT whose tumors express low levels of p16. PMID:21871868

  10. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Northwest Arctic, Alaska: FISHL (Fish Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for anadromous fish species in Northwest Arctic, Alaska. Vector arcs in this data set represent species...

  11. Hawaii ESI: ESI (Environmental Sensitivity Index Shoreline Types - Polygons and Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector arcs and polygons representing the shoreline and coastal habitats of Hawaii classified according to the Environmental Sensitivity Index...

  12. Highly-sensitive gas pressure sensor using twin-core fiber based in-line Mach-Zehnder interferometer.

    Science.gov (United States)

    Li, Zhengyong; Liao, Changrui; Wang, Yiping; Xu, Lei; Wang, Dongning; Dong, Xiaopeng; Liu, Shen; Wang, Qiao; Yang, Kaiming; Zhou, Jiangtao

    2015-03-09

    A Mach-Zehnder interferometer based on a twin-core fiber was proposed and experimentally demonstrated for gas pressure measurements. The in-line Mach-Zehnder interferometer was fabricated by splicing a short section of twin-core fiber between two single mode fibers. A micro-channel was created to form an interferometer arm by use of a femtosecond laser to drill through one core of the twin-core fiber. The other core of the fiber was remained as the reference arm. Such a Mach-Zehnder interferometer exhibited a high gas pressure sensitivity of -9.6 nm/MPa and a low temperature cross-sensitivity of 4.4 KPa/°C. Moreover, ultra-compact device size and all-fiber configuration make it very suitable for highly-sensitive gas pressure sensing in harsh environments.

  13. Improved limits on spin-dependent WIMP-proton interactions from a two liter CF3I bubble chamber.

    Science.gov (United States)

    Behnke, E; Behnke, J; Brice, S J; Broemmelsiek, D; Collar, J I; Cooper, P S; Crisler, M; Dahl, C E; Fustin, D; Hall, J; Hinnefeld, J H; Hu, M; Levine, I; Ramberg, E; Shepherd, T; Sonnenschein, A; Szydagis, M

    2011-01-14

    Data from the operation of a bubble chamber filled with 3.5 kg of CF3I in a shallow underground site are reported. An analysis of ultrasound signals accompanying bubble nucleations confirms that alpha decays generate a significantly louder acoustic emission than single nuclear recoils, leading to an efficient background discrimination. Three dark matter candidate events were observed during an effective exposure of 28.1  kg  day, consistent with a neutron background. This observation provides strong direct detection constraints on weakly interacting massive particle (WIMP)-proton spin-dependent scattering for WIMP masses >20  GeV/c2.

  14. A Search of Low-Mass WIMPs with p-type Point Contact Germanium Detector in the CDEX-1 Experiment

    CERN Document Server

    Zhao, W; Kang, K J; Cheng, J P; Li, Y J; Wong, H T; Lin, S T; Chang, J P; Chen, J H; Chen, Q H; Chen, Y H; Deng, Z; Du, Q; Gong, H; Hao, X Q; He, H J; He, Q J; Huang, H X; Huang, T R; Jiang, H; Li, H B; Li, J; Li, J M; Li, X; Li, X Y; Li, Y L; Lin, F K; Liu, S K; Lü, L C; Ma, H; Ma, J L; Mao, S J; Qin, J Q; Ren, J; Ruan, X C; Sharma, V; Shen, M B; Singh, L; Singh, M K; Soma, A K; Su, J; Tang, C J; Wang, J M; Wang, L; Wang, Q; Wu, S Y; Wu, Y C; Xianyu, Z Z; Xiao, R Q; Xing, H Y; Xu, F Z; Xu, Y; Xu, X J; Xue, T; Yang, L T; Yang, S W; Yi, N; Yu, C X; Yu, H; Yu, X Z; Zeng, M; Zeng, X H; Zeng, Z; Zhang, L; Zhang, Y H; Zhao, M G; Zhou, Z Y; Zhu, J J; Zhu, W B; Zhu, X Z; Zhu, Z H

    2016-01-01

    The CDEX-1 experiment conducted a search of low-mass (< 10 GeV/c2) Weakly Interacting Massive Particles (WIMPs) dark matter at the China Jinping Underground Laboratory using a p-type point-contact germanium detector with a fiducial mass of 915 g at a physics analysis threshold of 475 eVee. We report the hardware set-up, detector characterization, data acquisition and analysis procedures of this experiment. No excess of unidentified events are observed after subtraction of known background. Using 335.6 kg-days of data, exclusion constraints on the WIMP-nucleon spin-independent and spin-dependent couplings are derived.

  15. Consequences of DM/antiDM Oscillations for Asymmetric WIMP Dark Matter

    CERN Document Server

    Cirelli, Marco; Servant, Geraldine; Zaharijas, Gabrijela

    2012-01-01

    Assuming the existence of a primordial asymmetry in the dark sector, a scenario usually dubbed Asymmetric Dark Matter (aDM), we study the effect of oscillations between dark matter and its antiparticle on the re-equilibration of the initial asymmetry before freeze-out, which enable efficient annihilations to recouple. We calculate the evolution of the DM relic abundance and show how oscillations re-open the parameter space of aDM models, in particular in the direction of allowing large (WIMP-scale) DM masses. A typical wimp with a mass at the EW scale (\\sim 100 GeV - 1 TeV) presenting a primordial asymmetry of the same order as the baryon asymmetry naturally gets the correct relic abundance if the DM-number-violating Delta(DM) = 2 mass term is in the \\sim meV range. The re-establishment of annihilations implies that constraints from the accumulation of aDM in astrophysical bodies are evaded. On the other hand, the ordinary bounds from BBN, CMB and indirect detection signals on annihilating DM have to be consi...

  16. Dark matter spin-dependent limits for WIMP interactions on {sup 19}F by PICASSO

    Energy Technology Data Exchange (ETDEWEB)

    Archambault, S.; Aubin, F.; Auger, M. [Departement de Physique, Universite de Montreal, Montreal, H3C 3J7 (Canada); Behnke, E. [Department of Physics and Astronomy, Indiana University South Bend, South Bend, IN 46634 (United States); Beltran, B. [Department of Physics, University of Alberta, Edmonton, T6G 2G7 (Canada); Clark, K.; Dai, X.; Davour, A. [Department of Physics, Queen' s University, Kingston, K7L 3NG (Canada); Farine, J. [Department of Physics, Laurentian University, Sudbury, P3E 2C6 (Canada); Faust, R.; Genest, M.-H.; Giroux, G.; Gornea, R. [Departement de Physique, Universite de Montreal, Montreal, H3C 3J7 (Canada); Krauss, C. [Department of Physics, University of Alberta, Edmonton, T6G 2G7 (Canada); Kumaratunga, S. [Departement de Physique, Universite de Montreal, Montreal, H3C 3J7 (Canada); Lawson, I. [SNOLAB, Sudbury, P3E 2C6 (Canada); Leroy, C.; Lessard, L. [Departement de Physique, Universite de Montreal, Montreal, H3C 3J7 (Canada); Levy, C. [Department of Physics, Queen' s University, Kingston, K7L 3NG (Canada); Levine, I. [Department of Physics and Astronomy, Indiana University South Bend, South Bend, IN 46634 (United States)

    2009-11-30

    The PICASSO experiment at SNOLAB reports new results for spin-dependent WIMP interactions on {sup 19}F using the superheated droplet technique. A new generation of detectors and new features which enable background discrimination via the rejection of non-particle induced events are described. First results are presented for a subset of two detectors with target masses of {sup 19}F of 65 g and 69 g respectively and a total exposure of 13.75+-0.48 kgd. No dark matter signal was found and for WIMP masses around 24 GeV/c{sup 2} new limits have been obtained on the spin-dependent cross section on {sup 19}F of sigma{sub F}=13.9 pb (90% C.L.) which can be converted into cross section limits on protons and neutrons of sigma{sub p}=0.16 pb and sigma{sub n}=2.60 pb respectively (90% C.L.). The obtained limits on protons restrict recent interpretations of the DAMA/LIBRA annual modulations in terms of spin-dependent interactions.

  17. Dark Matter Spin-Dependent Limits for WIMP Interactions on 19-F by PICASSO

    CERN Document Server

    Archambault, S; Auger, M; Behnke, E; Beltran, B; Clark, K; Dai, X; Davour, A; Farine, J; Faust, R; Genest, M-H; Giroux, G; Gornea, R; Krauss, C; Kumaratunga, S; Lawson, I; Leroy, C; Lessard, L; Levy, C; Levine, I; MacDonald, R; Martin, J -P; Nadeau, P; Noble, A; Piro, M -C; Pospísil, S; Shepherd, T; Starinski, N; Stekl, I; Storey, C; Wichoski, U; Zacek, V

    2009-01-01

    The PICASSO experiment at SNOLAB reports new results for spin-dependent WIMP interactions on $^{19}$F using the superheated droplet technique. A new generation of detectors and new features which enable background discrimination via the rejection of non-particle induced events are described. First results are presented for a subset of two detectors with target masses of $^{19}$F of 65 g and 69 g respectively and a total exposure of 13.75 $\\pm$ 0.48 kgd. No dark matter signal was found and for WIMP masses around 24 GeV/c$^2$ new limits have been obtained on the spin-dependent cross section on $^{19}$F of $\\sigma_F$ = 13.9 pb (90% C.L.) which can be converted into cross section limits on protons and neutrons of $\\sigma_p$ = 0.16 pb and $\\sigma_n$ = 2.60 pb respectively (90% C.L). The obtained limits on protons restrict recent interpretations of the DAMA/LIBRA annual modulations in terms of spin-dependent interactions.

  18. Human cell line sensitive to mutation by particle-borne chemicals

    Energy Technology Data Exchange (ETDEWEB)

    Crespi, C.L.; Liber, H.L.; Behymer, T.D.; Hites, R.A.; Thilly, W.G.

    1985-07-01

    A human lymphoblastoid cell line with ability to perform oxidative metabolism of various chemicals is mutated by the direct addition of an intact particulate soot. This experiment demonstrates that materials associated with combustion-generated particulates are biologically available and able to cause genetic changes in metabolically competent human cells.

  19. Sensitivity to ionizing radiation and chemotherapeutic agents in gemcitabine-resistant human tumor cell lines

    NARCIS (Netherlands)

    van Bree, Chris; Castro Kreder, Natasja; Loves, Willem J. P.; Franken, Nicolaas A. P.; Peters, Godefridus J.; Haveman, Jaap

    2002-01-01

    Purpose: To determine cross-resistance to anti-tumor treatments in 2',2'difluorodeoxycytidine (dFdC, gemcitabine)-resistant human tumor cells. Methods and Materials: Human lung carcinoma cells SW-1573 (SWp) were made resistant to dFdC (SWg). Sensitivity to cisplatin (cDDP), paclitaxel,

  20. The sensitivities to first-line antibiotic therapy of the common urinary ...

    African Journals Online (AJOL)

    Unlike the Staphylococcus species that were significantly resistant to nitrofurantoin, Streptococcus species were moderately susceptible to the drug. Conclusion: The common urinary tract bacteria detected in Rubaga hospital in Uganda were most sensitive to Ciprofloxacin and Nitrofurantoin. African Health Sciences Vol.

  1. TERRA Expression Levels Do Not Correlate With Telomere Length and Radiation Sensitivity in Human Cancer Cell Lines

    Directory of Open Access Journals (Sweden)

    Alexandra eSmirnova

    2013-05-01

    Full Text Available Mammalian telomeres are transcribed into long non-coding telomeric RNA molecules (TERRA that seem to play a role in the maintenance of telomere stability. In human cells, CpG island promoters drive TERRA transcription and are regulated by methylation. It was suggested that the amount of TERRA may be related to telomere length. To test this hypothesis we measured telomere length and TERRA levels in single clones isolated from five human cell lines: HeLa (cervical carcinoma, BRC-230 (breast cancer, AKG and GK2 (gastric cancers and GM847 (SV40 immortalized skin fibroblasts. We observed great clonal heterogeneity both in TRF (Terminal Restriction Fragment length and in TERRA levels. However, these two parameters did not correlate with each other. Moreover, cell survival to γ-rays did not show a significant variation among the clones, suggesting that, in this cellular system, the intra-population variability in telomere length and TERRA levels does not influence sensitivity to ionizing radiation. This conclusion was supported by the observation that in a cell line in which telomeres were greatly elongated by the ectopic expression of telomerase, TERRA expression levels and radiation sensitivity were similar to the parental HeLa cell line.

  2. TERRA Expression Levels Do Not Correlate with Telomere Length and Radiation Sensitivity in Human Cancer Cell Lines.

    Science.gov (United States)

    Smirnova, Alexandra; Gamba, Riccardo; Khoriauli, Lela; Vitelli, Valerio; Nergadze, Solomon G; Giulotto, Elena

    2013-01-01

    Mammalian telomeres are transcribed into long non-coding telomeric repeat-containing RNA (TERRA) molecules that seem to play a role in the maintenance of telomere stability. In human cells, CpG-island promoters drive TERRA transcription and are regulated by methylation. It was suggested that the amount of TERRA may be related to telomere length. To test this hypothesis we measured telomere length and TERRA levels in single clones isolated from five human cell lines: HeLa (cervical carcinoma), BRC-230 (breast cancer), AKG and GK2 (gastric cancers), and GM847 (SV40 immortalized skin fibroblasts). However, these two parameters did not correlate with each other. Moreover, cell survival to γ-rays did not show a significant variation among the clones, suggesting that, in this cellular system, the intra-population variability in telomere length and TERRA levels does not influence sensitivity to ionizing radiation. This conclusion was supported by the observation that in a cell line in which telomeres were greatly elongated by the ectopic expression of telomerase, TERRA expression levels and radiation sensitivity were similar to the parental HeLa cell line.

  3. Human Adenocarcinoma Cell Line Sensitivity to Essential Oil Phytocomplexes from Pistacia Species: a Multivariate Approach.

    Science.gov (United States)

    Buriani, Alessandro; Fortinguerra, Stefano; Sorrenti, Vincenzo; Dall'Acqua, Stefano; Innocenti, Gabbriella; Montopoli, Monica; Gabbia, Daniela; Carrara, Maria

    2017-08-11

    Principal component analysis (PCA) multivariate analysis was applied to study the cytotoxic activity of essential oils from various species of the Pistacia genus on human tumor cell lines. In particular, the cytotoxic activity of essential oils obtained from P. lentiscus, P. lentiscus var. chia (mastic gum), P. terebinthus, P. vera, and P. integerrima, was screened on three human adenocarcinoma cell lines: MCF-7 (breast), 2008 (ovarian), and LoVo (colon). The results indicate that all the Pistacia phytocomplexes, with the exception of mastic gum oil, induce cytotoxic effects on one or more of the three cell lines. PCA highlighted the presence of different cooperating clusters of bioactive molecules. Cluster variability among species, and even within the same species, could explain some of the differences seen among samples suggesting the presence of both common and species-specific mechanisms. Single molecules from one of the most significant clusters were tested, but only bornyl-acetate presented cytotoxic activity, although at much higher concentrations (IC50 = 138.5 µg/mL) than those present in the essential oils, indicating that understanding of the full biological effect requires a holistic vision of the phytocomplexes with all its constituents.

  4. Response of sensitive human ataxia and resistant T-1 cell lines to accelerated heavy ions

    Energy Technology Data Exchange (ETDEWEB)

    Tobias, C.A.; Blakely, E.A.; Chang, P.Y.; Lommel, L.; Roots, R.

    1983-07-01

    The radiation dose responses of fibroblast from a patient with Ataxia telangiectasis (AT-2SF) and an established line of human T-1 cells were studied. Nearly monoenergetic accelerated neon and argon ions were used at the Berkeley Bevalac with various residual range values. The LET of the particles varied from 30 keV/..mu..m to over 1000 keV/..mu..m. All Ataxia survival curves were exponential functions of the dose. Their radiosensitivity reached peak values at 100 to 200 keV/..mu..m. Human T-1 cells have effective sublethal damage repair as has been evidenced by split dose experiments, and they are much more resistant to low LET than to high LET radiation. The repair-misrepair model has been used to interpret these results. We have obtained mathematical expressions that describe the cross sections and inactivation coefficients for both human cell lines as a function of the LET and the type of particle used. The results suggest either that high-LET particles induce a greater number of radiolesions per track or that heavy-ions at high LET induce lesions that kill cells more effectively and that are different from those produced at low LET. We assume that the lesions induced in T-1 and Ataxia cells are qualitatively similar and that each cell line attempts to repair these lesions. The result in most irradiated Ataxia cells, however, is either lethal misrepair or incomplete repair leading to cell death. 63 references, 10 figures, 1 table.

  5. A sensitive and robust method for automated on-line monitoring of enzymatic activities in water and water resources.

    Science.gov (United States)

    Ryzinska-Paier, G; Lendenfeld, T; Correa, K; Stadler, P; Blaschke, A P; Mach, R L; Stadler, H; Kirschner, A K T; Farnleitner, A H

    2014-01-01

    The realisation of a novel concept for automated on-line monitoring of enzymatic activities in water was successfully demonstrated by long-term field testing at two remote Austrian ground water resources. The β-D-glucuronidase (GLUC) activity was selected as a representative enzymatic model parameter for the on-line determination. But the device can be adapted for any enzymatic reaction with diagnostic relevance for microbial water quality monitoring, as demonstrated for the β-D-galactosidase activity. Automated filtration of volumes up to 5 litres supports sensitive quantification of enzymatic activities. Internet-based data transfer, using internal control parameters for verification and a dynamic determination of the limit of quantification, enabled robust enzymatic on-line monitoring during a 2-year period. A proportion of 5,313 out of 5,506 GLUC activity measurements (96.5%) could be positively verified. Hydrological (discharge, gauge, turbidity, temperature, pH, electric conductivity, spectral absorbance coefficient at 254 nm) as well as microbiological parameters (Escherichia coli, coliforms) were concurrently determined to characterise the investigated ground water resources. The enzymatic on-line measurements closely reflected the different hydrological conditions and contamination patterns of the test sites. Contrary to expectations, GLUC did not qualify as a proxy-parameter for the occurrence of cultivation-based E. coli contamination and warrants further detailed investigations on its indication capacity as a rapid means for microbial faecal pollution detection in such aquatic habitats. Microbial on-line monitoring is likely to become more important in the future, complementing existing surveillance strategies for water safety management. Further perspectives on the application of such analytical on-line technologies, such as their connection with event-triggered sampling and standardised diagnostics, are discussed.

  6. A Bio-Inspired Model-Based Approach for Context-Aware Post-WIMP Tele-Rehabilitation

    Directory of Open Access Journals (Sweden)

    Víctor López-Jaquero

    2016-10-01

    Full Text Available Tele-rehabilitation is one of the main domains where Information and Communication Technologies (ICT have been proven useful to move healthcare from care centers to patients’ home. Moreover, patients, especially those carrying out a physical therapy, cannot use a traditional Window, Icon, Menu, Pointer (WIMP system, but they need to interact in a natural way, that is, there is a need to move from WIMP systems to Post-WIMP ones. Moreover, tele-rehabilitation systems should be developed following the context-aware approach, so that they are able to adapt to the patients’ context to provide them with usable and effective therapies. In this work a model-based approach is presented to assist stakeholders in the development of context-aware Post-WIMP tele-rehabilitation systems. It entails three different models: (i a task model for designing the rehabilitation tasks; (ii a context model to facilitate the adaptation of these tasks to the context; and (iii a bio-inspired presentation model to specify thoroughly how such tasks should be performed by the patients. Our proposal overcomes one of the limitations of the model-based approach for the development of context-aware systems supporting the specification of non-functional requirements. Finally, a case study is used to illustrate how this proposal can be put into practice to design a real world rehabilitation task.

  7. Photographic but not line-drawn faces show early perceptual neural sensitivity to eye gaze direction

    Directory of Open Access Journals (Sweden)

    Alejandra eRossi

    2015-04-01

    Full Text Available Our brains readily decode facial movements and changes in social attention, reflected in earlier and larger N170 event-related potentials (ERPs to viewing gaze aversions vs. direct gaze in real faces (Puce et al. 2000. In contrast, gaze aversions in line-drawn faces do not produce these N170 differences (Rossi et al., 2014, suggesting that physical stimulus properties or experimental context may drive these effects. Here we investigated the role of stimulus-induced context on neurophysiological responses to dynamic gaze. Sixteen healthy adults viewed line-drawn and real faces, with dynamic eye aversion and direct gaze transitions, and control stimuli (scrambled arrays and checkerboards while continuous electroencephalographic (EEG activity was recorded. EEG data from 2 temporo-occipital clusters of 9 electrodes in each hemisphere where N170 activity is known to be maximal were selected for analysis. N170 peak amplitude and latency, and temporal dynamics from event-related spectral perturbations (ERSPs were measured in 16 healthy subjects. Real faces generated larger N170s for averted vs. direct gaze motion, however, N170s to real and direct gaze were as large as those to respective controls. N170 amplitude did not differ across line-drawn gaze changes. Overall, bilateral mean gamma power changes for faces relative to control stimuli occurred between 150-350 ms, potentially reflecting signal detection of facial motion.Our data indicate that experimental context does not drive N170 differences to viewed gaze changes. Low-level stimulus properties, such as the high sclera/iris contrast change in real eyes likely drive the N170 changes to viewed aversive movements.

  8. Chromatin status of apoptosis genes correlates with sensitivity to chemo-, immune- and radiation therapy in colorectal cancer cell lines.

    Science.gov (United States)

    Benard, Anne; Janssen, Connie M; van den Elsen, Peter J; van Eggermond, Marja C J A; Hoon, Dave S B; van de Velde, Cornelis J H; Kuppen, Peter J K

    2014-12-01

    The apoptosis pathway of programmed cell death is frequently deregulated in cancer. An intact apoptosis pathway is required for proper response to anti-cancer treatment. We investigated the chromatin status of key apoptosis genes in the apoptosis pathway in colorectal cancer cell lines in relation to apoptosis induced by chemo-, immune- or radiation therapy. Using chromatin immunoprecipitation (ChIP), we measured the presence of transcription-activating histone modifications H3Ac and H3K4me3 and silencing modifications H3K9me3 and H3K27me3 at the gene promoter regions of key apoptosis genes Bax, Bcl2, Caspase-9, Fas (CD95) and p53. Cell lines DLD1, SW620, Colo320, Caco2, Lovo and HT29 were treated with cisplatin, anti-Fas or radiation. The apoptotic response was measured by flow cytometry using propidium iodide and annexin V-FITC. The chromatin status of the apoptosis genes reflected the activation status of the intrinsic (Bax, Bcl2, Caspase-9 and p53) and extrinsic (Fas) pathways. An active intrinsic apoptotic pathway corresponded to sensitivity to cisplatin and radiation treatment of cell lines DLD1, SW620 and Colo320. An active Fas promoter corresponded to an active extrinsic apoptotic pathway in cell line DLD1. mRNA expression data correlated with the chromatin status of the apoptosis genes as measured by ChIP. In conclusion, the results presented in this study indicate that the balance between activating and silencing histone modifications, reflecting the chromatin status of apoptosis genes, can be used to predict the response of tumor cells to different anti-cancer therapies and could provide a novel target to sensitize tumors to obtain adequate treatment responses.

  9. Identification of proteins sensitive to thermal stress in human neuroblastoma and glioma cell lines.

    Directory of Open Access Journals (Sweden)

    Guilian Xu

    Full Text Available Heat-shock is an acute insult to the mammalian proteome. The sudden elevation in temperature has far-reaching effects on protein metabolism, leads to a rapid inhibition of most protein synthesis, and the induction of protein chaperones. Using heat-shock in cells of neuronal (SH-SY5Y and glial (CCF-STTG1 lineage, in conjunction with detergent extraction and sedimentation followed by LC-MS/MS proteomic approaches, we sought to identify human proteins that lose solubility upon heat-shock. The two cell lines showed largely overlapping profiles of proteins detected by LC-MS/MS. We identified 58 proteins in detergent insoluble fractions as losing solubility in after heat shock; 10 were common between the 2 cell lines. A subset of the proteins identified by LC-MS/MS was validated by immunoblotting of similarly prepared fractions. Ultimately, we were able to definitively identify 3 proteins as putatively metastable neural proteins; FEN1, CDK1, and TDP-43. We also determined that after heat-shock these cells accumulate insoluble polyubiquitin chains largely linked via lysine 48 (K-48 residues. Collectively, this study identifies human neural proteins that lose solubility upon heat-shock. These proteins may represent components of the human proteome that are vulnerable to misfolding in settings of proteostasis stress.

  10. High sensitive FBG load cell for icing of overhead transmission lines

    Science.gov (United States)

    Mao, Naiqiang; Ma, Guoming; Li, Chengrong; Li, Yabo; Shi, Cheng; Du, Yue

    2017-04-01

    Heavy ice coating of overhead transmission lines created the serious threat on the safe operation of power grid. The measurement of conductor icing had been an effective and reliable methods to prevent potential risks, such as conductor breakage, insulator flashover and tower collapse. Because of the advantages of immunity to electromagnetic interference and no demand for power supply in site, the optical load cell has been widely applied in monitoring the ice coating of overhead transmission lines. In this paper, we have adopted the shearing structure with additional grooves as elastic element of load cell to detect the eccentric load. Then, two welding package fiber Bragg gratings (FBGs) were mounted onto the grooves of elastic element with a direction deviation of 90° to eliminate temperature effects on strain measurement without extra FBG. After that, to avoid the occurrence of load cell breakage in heavy load measurement, the protection part has been proposed and added to the elastic element. The results of tension experiments indicate that the resolution of the load cell is 7.78 N in the conventional measuring range (0-10 kN). And in addition, the load cell proposed in this paper also has a good performance in actual experiment in which the load and temperature change simultaneously.

  11. Paris Saponin I Sensitizes Gastric Cancer Cell Lines to Cisplatin via Cell Cycle Arrest and Apoptosis

    OpenAIRE

    Song, Shuichuan; Du, Leiwen; Jiang, Hao; Zhu, Xinhai; Li, Jinhui; Xu, Ji

    2016-01-01

    Background Dose-related toxicity is the major restriction of cisplatin and cisplatin-combination chemotherapy, and is a challenge for advanced gastric cancer treatment. We explored the possibility of using Paris saponin I as an agent to sensitize gastric cancer cells to cisplatin, and examined the underlying mechanism. Material/Methods Growth inhibition was detected by MTT assay. The cell cycle and apoptosis were detected using flow cytometry and Annexin V/PI staining. The P21waf1/cip1, Bcl-2...

  12. Characterization of Adriamycin-resistant and radiation-sensitive Chinese hamster cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Sognier, M.A.; Yin Zhang; Eberle, R.L.; Belli, J.A. (Texas Univ., Galveston, TX (United States). Medical Branch)

    1992-11-03

    A series of cell lines derived from Chinese hamster V79 cells by selection in increasing concentrations of Adriamycin (ADRM) was developed to study the mechanism of drug resistance and its relationship to radiation response. Survival studies revealed that selection in increasingly higher concentrations of ADRM positively correlated with increased cellular drug resistance. Increased cellular resistance correlated positively with amplification of the hamster multidrug-resistance gene (pgp 1) as detected with dot blot analysis using the pCHP1 probe. Southern blot analysis of restriction endonuclease digested DNA showed that (1) some fragments were preferentially amplified compared to others in the ADRM-resistant; and (2) no major gene rearrangement appeared to have occurred during the selection for greater ADRM resistance. (author).

  13. arXiv Wilson lines and UV sensitivity in magnetic compactifications

    CERN Document Server

    Ghilencea, D.M.

    2017-06-07

    We investigate the ultraviolet (UV) behaviour of 6D N=1 supersymmetric effective (Abelian) gauge theories compactified on a two-torus (T$_{2}$) with magnetic flux. To this purpose we compute offshell the one-loop correction to the Wilson line state self-energy. The offshell calculation is actually necessary to capture the usual effective field theory expansion in powers of (∂/Λ). Particular care is paid to the regularization of the (divergent) momentum integrals, which is relevant for identifying the corresponding counterterm(s). We find a counterterm which is a new higher dimensional effective operator of dimension d=6, that is enhanced for a larger compactification area (where the effective theory applies) and is consistent with the symmetries of the theory. Its consequences are briefly discussed and comparison is made with orbifold compactifications without flux.

  14. Position and time sensitive photon counting detector with image charge delay-line readout

    Science.gov (United States)

    Czasch, Achim; Dangendorf, Volker; Milnes, James; Schössler, Sven; Lauck, Ronald; Spillmann, Uwe; Howorth, Jon; Jagutzki, Ottmar

    2007-09-01

    We have developed single photon counting image intensifier tubes combining position and time information read-out with at least 500x500 pixels and sub-nanosecond time resolution. This image intensifier type uses a resistive screen instead of a phosphor screen and the image charge pickup anode is placed outside the sealed tube. We present a novel delay-line anode design which allows for instance detecting simultaneously arriving pairs of photons. Due to the very low background this technique is suited for applications with very low light intensity and especially if a precise time tagging for each photon is required. We show results obtained with several anode types on a 25 mm image intensifier tube and a 40 mm open-face MCP detector and discuss the performance in neutron radiography, e.g. for homeland security, and the prospects for applications like Fluorescence Life-time Imaging Microscopy (FLIM), astronomy and X-ray polarimetry.

  15. Tests of WIMP Dark Matter Candidates with Direct Dark Matter Detection Experiments

    Science.gov (United States)

    Georgescu, Andreea Irina

    We reexamine the current direct dark matter (DM) detection data for several types of DM candidates, both assuming the Standard Halo Model (SHM) and in a halo-independent manner. We consider the potential signals for light WIMPs that have appeared in three direct detection searches: DAMA, CDMS-II-Si, and CoGeNT, and we analyze their compatibility with the null results of other direct detection experiments. We first consider light WIMPs with exothermic scattering with nuclei (exoDM). Exothermic interactions favor light targets, thus reducing the importance of upper limits derived from Xe targets, the most restrictive of which is at present the LUX limit. In our SHM analysis the CDMS-II-Si and CoGeNT regions become allowed by these bounds, however the SuperCDMS limit rejects both regions for exoDM with isospin-conserving couplings. An isospin-violating coupling of the exoDM, in particular one with a neutron to proton coupling ratio of -0.8 (which we call "Ge-phobic"), maximally reduces the DM coupling to Ge and allows the CDMS-II-Si region to become compatible with all upper bounds. This is also clearly shown in our halo-independent analysis. Next, we extend and correct a recently proposed maximum-likelihood halo-independent method to analyze unbinned direct DM detection data. Instead of the recoil energy as an independent variable, we use the minimum speed a DM particle must have to impart a given recoil energy to a nucleus. This has the advantage of allowing us to apply the method to any type of target composition and interaction, e.g. with general momentum and velocity dependence, and with elastic or inelastic scattering. We prove the method and provide a rigorous statistical interpretation of the results. As first applications, we find that for dark matter particles with elastic spin-independent interactions and neutron to proton coupling ratio ƒn/ƒp = --0.7 ("Xe-phobic", which reduces maximally the coupling to Xe), the WIMP interpretation of the signal observed

  16. Critical System Cascading Collapse Assessment for Determining the Sensitive Transmission Lines and Severity of Total Loading Conditions

    Directory of Open Access Journals (Sweden)

    Nur Ashida Salim

    2013-01-01

    Full Text Available This paper presents a computationally accurate technique used to determine the estimated average probability of a system cascading collapse considering the effect of hidden failure on a protection system. This includes an accurate calculation of the probability of hidden failure as it will give significant effect on the results of the estimated average probability of system cascading collapse. The estimated average probability of a system cascading collapse is then used to determine the severe loading condition contributing to a higher risk of a system cascading collapse. This information is important because it will assist the utility to determine the maximum level of increase in the system loading condition before the occurrence of critical power system cascading collapse. Furthermore, the initial tripping of sensitive transmission line contributing to a critical system cascading collapse can also be determined by using the proposed method. Based on the results obtained from this study, it was found that selecting the accurate probability of hidden failure is very important as it will affect the estimated average probability of a system cascading collapse. Comparative study has been done with other techniques to verify the effectiveness of the proposed method used in the determination of sensitive transmission lines.

  17. 7th Patras workshop on axions, WIMPs and WISPs (PATRAS 2011). Proceedings

    Energy Technology Data Exchange (ETDEWEB)

    Zioutas, Konstantin; Schumann, Marc (eds.)

    2011-12-15

    The year 2011 was an exciting period to work on the ''dark side'' of the Universe. Several experimental claims of the direct detection of WIMP dark matter were challenged by the restrictive limits of several others. Dedicated experiments searching for axions, another well motivated dark matter candidate, and for axion-like particles continued to improve their limits and got an additional boost by puzzling astrophysical observations and new developments in theory. The LHC collected an unexpectedly large amount of data and started to produce results at an amazing speed. And finally, this year's Nobel price of physics was awarded to the observation of the accelerating expansion of the Universe, an effect which might be related to dark energy, whose nature remain among the biggest mysteries in physics. These exciting topics and many more important aspects of particle- and astroparticle physics were discussed between experimentalists and theorists at the 7th Patras Workshop on Axions, WIMPs, and WISPs. The workshop took place from June 27 - July 1, 2011, in the Royal Myconian and Myconian Imperial Resorts Hotels on the Greek island of Mykonos. As in the previous years, it was a very fruitful and lively meeting in an inspiring and open atmosphere, which allowed for many open and constructive discussions also on controversial topics. The scientific exchange, the beautiful scenery of the island, the venue itself, the food, an excursion to the ancient ruins of Delos, and finally an amazing conference dinner made this meeting really unique. The ''spirit'' of the workshop and its atmosphere cannot be brought to paper, but many of its scientific highlights are collected in these proceedings. We are looking forward to the 8th Patras Workshop, which will be held in Chicago (USA) July 18-22, 2012. It will be organized jointly by our US colleagues Andrei Afanasev (JLAB), Oliver Baker (Yale), and William Wester (FNAL).

  18. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Puget Sound and Strait of Juan de Fuca, Washington: HABITATL (Habitat Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for marine and estuarine vegetation in Puget Sound and Strait of Juan de Fuca, Washington. Vector lines in...

  19. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Puget Sound and Strait of Juan de Fuca, Washington: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for Puget Sound...

  20. A Sensitive Method for Cadmium Determination Using an On-line Polyurethane Foam Preconcentration System and Thermospray Flame Furnace Atomic Absorption Spectrometry

    National Research Council Canada - National Science Library

    TARLEY, César Ricardo Teixeira; ARRUDA, Marco Aurélio Zezzi

    2004-01-01

    A new sensitive and low cost method for cadmium determination at µg l-1 levels that combines an on-line preconcentration system with the thermospray flame furnace atomic absorption spectrometry technique (TS-FF-AAS...

  1. Mycoplasma hyorhinis-Contaminated Cell Lines Activate Primary Innate Immune Cells via a Protease-Sensitive Factor.

    Directory of Open Access Journals (Sweden)

    Simon Heidegger

    Full Text Available Mycoplasma are a frequent and occult contaminant of cell cultures, whereby these prokaryotic organisms can modify many aspects of cell physiology, rendering experiments that are conducted with such contaminated cells problematic. Chronic Mycoplasma contamination in human monocytic cells lines has been associated with suppressed Toll-like receptor (TLR function. In contrast, we show here that components derived from a Mycoplasma hyorhinis-infected cell line can activate innate immunity in non-infected primary immune cells. Release of pro-inflammatory cytokines such as IL-6 by dendritic cells in response to Mycoplasma hyorhinis-infected cell components was critically dependent on the adapter protein MyD88 but only partially on TLR2. Unlike canonical TLR2 signaling that is triggered in response to the detection of Mycoplasma infection, innate immune activation by components of Mycoplasma-infected cells was inhibited by chloroquine treatment and sensitive to protease treatment. We further show that in plasmacytoid dendritic cells, soluble factors from Mycoplasma hyorhinis-infected cells induce the production of large amounts of IFN-α. We conclude that Mycoplasma hyorhinis-infected cell lines release protein factors that can potently activate co-cultured innate immune cells via a previously unrecognized mechanism, thus limiting the validity of such co-culture experiments.

  2. Cultural differences in on-line sensitivity to emotional voices: Comparing East and West

    Directory of Open Access Journals (Sweden)

    Pan eLiu

    2015-05-01

    Full Text Available Evidence that culture modulates on-line neural responses to the emotional meanings encoded by vocal and facial expressions was demonstrated recently in a study comparing English North Americans and Chinese (Liu et al., 2015. Here, we compared how individuals from these two cultures passively respond to emotional cues from faces and voices using an Oddball task. Participants viewed in-group emotional faces, with or without simultaneous vocal expressions, while performing a face-irrelevant visual task as the EEG was recorded. A significantly larger visual MMN was observed for Chinese versus English participants when faces were accompanied by voices, suggesting that Chinese were influenced to a larger extent by task-irrelevant vocal cues. These data highlight further differences in how adults from East Asian versus Western cultures process socio-emotional cues, arguing that distinct cultural practices in communication (e.g., display rules shape neurocognitive activity associated with the early perception and integration of multi-sensory emotional cues.

  3. Bioinformatic analyses identifies novel protein-coding pharmacogenomic markers associated with paclitaxel sensitivity in NCI60 cancer cell lines.

    Science.gov (United States)

    Eng, Lawson; Ibrahim-zada, Irada; Jarjanazi, Hamdi; Savas, Sevtap; Meschian, Mehran; Pritchard, Kathleen I; Ozcelik, Hilmi

    2011-02-11

    Paclitaxel is a microtubule-stabilizing drug that has been commonly used in treating cancer. Due to genetic heterogeneity within patient populations, therapeutic response rates often vary. Here we used the NCI60 panel to identify SNPs associated with paclitaxel sensitivity. Using the panel's GI50 response data available from Developmental Therapeutics Program, cell lines were categorized as either sensitive or resistant. PLINK software was used to perform a genome-wide association analysis of the cellular response to paclitaxel with the panel's SNP-genotype data on the Affymetrix 125 k SNP array. FastSNP software helped predict each SNP's potential impact on their gene product. mRNA expression differences between sensitive and resistant cell lines was examined using data from BioGPS. Using Haploview software, we investigated for haplotypes that were more strongly associated with the cellular response to paclitaxel. Ingenuity Pathway Analysis software helped us understand how our identified genes may alter the cellular response to paclitaxel. 43 SNPs were found significantly associated (FDRlines. Haplotypes found in GRIK1, SGCD, ROBO1, LPHN2, and PTPRD were more strongly associated with response than their individual SNPs. Our study has taken advantage of available genotypic data and its integration with drug response data obtained from the NCI60 panel. We identified 10 SNPs located within protein-coding genes that were not previously shown to be associated with paclitaxel response. As only five genes showed differential mRNA expression, the remainder would not have been detected solely based on expression data. The identified haplotypes highlight the role of utilizing SNP combinations within genomic loci of interest to improve the risk determination associated with drug response. These genetic variants represent promising biomarkers for predicting paclitaxel response and may play a significant role in the cellular response to paclitaxel.

  4. Inhibition of mTOR promotes hyperthermia sensitivity in SMMC-7721 human hepatocellular carcinoma cell line

    Science.gov (United States)

    WANG, QING-LIANG; LIU, BO; LI, XIAO-JIE; HU, KUN-PENG; ZHAO, KUN; YE, XIAO-MING

    2016-01-01

    The mammalian target of rapamycin (mTOR) is a critical mediator of the phosphoinositide 3-kinase/protein kinase B/mTOR signaling pathway, and mTOR activity is induced following heat shock. Thermotherapy is used to treat hepatocellular carcinoma (HCC). However, the role of mTOR in modulating thermosensitivity in HCC has yet to be elucidated. In the present study, the antisense plasmid pEGFP-C1-mTOR was transfected into SMMC-7721 cells, and the expression levels of mTOR were analyzed by reverse transcription-polymerase chain reaction and western blot analysis. The thermal responses of the transfected cells were also examined. The results revealed that SMMC-7721 cells were sensitive to heat treatment, and cell viability was significantly inhibited following hyperthermia treatment (P<0.01). The mRNA and protein expression levels of mTOR decreased post-transfection. Cell proliferation, colony-forming ability and motility were all significantly decreased following hyperthermia treatment in the transfected cells. Flow cytometry analysis demonstrated that apoptosis was significantly increased following treatment (P<0.01). The number of cells in S phase was increased, and the cell cycle was arrested in S phase. In conclusion, inhibition of mTOR increased the thermosensitivity of SMMC-7721 cells by increasing cellular apoptosis and inducing S phase arrest. PMID:26998020

  5. Protein Profiles Reveal Diverse Responsive Signaling Pathways in Kernels of Two Maize Inbred Lines with Contrasting Drought Sensitivity

    Directory of Open Access Journals (Sweden)

    Liming Yang

    2014-10-01

    Full Text Available Drought stress is a major factor that contributes to disease susceptibility and yield loss in agricultural crops. To identify drought responsive proteins and explore metabolic pathways involved in maize tolerance to drought stress, two maize lines (B73 and Lo964 with contrasting drought sensitivity were examined. The treatments of drought and well water were applied at 14 days after pollination (DAP, and protein profiles were investigated in developing kernels (35 DAP using iTRAQ (isobaric tags for relative and absolute quantitation. Proteomic analysis showed that 70 and 36 proteins were significantly altered in their expression under drought treatments in B73 and Lo964, respectively. The numbers and levels of differentially expressed proteins were generally higher in the sensitive genotype, B73, implying an increased sensitivity to drought given the function of the observed differentially expressed proteins, such as redox homeostasis, cell rescue/defense, hormone regulation and protein biosynthesis and degradation. Lo964 possessed a more stable status with fewer differentially expressed proteins. However, B73 seems to rapidly initiate signaling pathways in response to drought through adjusting diverse defense pathways. These changes in protein expression allow for the production of a drought stress-responsive network in maize kernels.

  6. HCPCF-based in-line fiber Fabry-Perot refractometer and high sensitivity signal processing method

    Science.gov (United States)

    Liu, Xiaohui; Jiang, Mingshun; Sui, Qingmei; Geng, Xiangyi; Song, Furong

    2017-12-01

    An in-line fiber Fabry-Perot interferometer (FPI) based on the hollow-core photonic crystal fiber (HCPCF) for refractive index (RI) measurement is proposed in this paper. The FPI is formed by splicing both ends of a short section of the HCPCF to single mode fibers (SMFs) and cleaving the SMF pigtail to a proper length. The RI response of the sensor is analyzed theoretically and demonstrated experimentally. The results show that the FPI sensor has linear response to external RI and good repeatability. The sensitivity calculated from the maximum fringe contrast is -136 dB/RIU. A new spectrum differential integration (SDI) method for signal processing is also presented in this study. In this method, the RI is obtained from the integrated intensity of the absolute difference between the interference spectrum and its smoothed spectrum. The results show that the sensitivity obtained from the integrated intensity is about -1.34×105 dB/RIU. Compared with the maximum fringe contrast method, the new SDI method can provide the higher sensitivity, better linearity, improved reliability, and accuracy, and it's also convenient for automatic and fast signal processing in real-time monitoring of RI.

  7. HCPCF-based in-line fiber Fabry-Perot refractometer and high sensitivity signal processing method

    Science.gov (United States)

    Liu, Xiaohui; Jiang, Mingshun; Sui, Qingmei; Geng, Xiangyi; Song, Furong

    2017-04-01

    An in-line fiber Fabry-Perot interferometer (FPI) based on the hollow-core photonic crystal fiber (HCPCF) for refractive index (RI) measurement is proposed in this paper. The FPI is formed by splicing both ends of a short section of the HCPCF to single mode fibers (SMFs) and cleaving the SMF pigtail to a proper length. The RI response of the sensor is analyzed theoretically and demonstrated experimentally. The results show that the FPI sensor has linear response to external RI and good repeatability. The sensitivity calculated from the maximum fringe contrast is-136 dB/RIU. A new spectrum differential integration (SDI) method for signal processing is also presented in this study. In this method, the RI is obtained from the integrated intensity of the absolute difference between the interference spectrum and its smoothed spectrum. The results show that the sensitivity obtained from the integrated intensity is about-1.34×105 dB/RIU. Compared with the maximum fringe contrast method, the new SDI method can provide the higher sensitivity, better linearity, improved reliability, and accuracy, and it's also convenient for automatic and fast signal processing in real-time monitoring of RI.

  8. A Sensitive Sensor Cell Line for the Detection of Oxidative Stress Responses in Cultured Human Keratinocytes

    Directory of Open Access Journals (Sweden)

    Ute Hofmann

    2014-06-01

    Full Text Available In the progress of allergic and irritant contact dermatitis, chemicals that cause the generation of reactive oxygen species trigger a heat shock response in keratinocytes. In this study, an optical sensor cell line based on cultured human keratinocytes (HaCaT cells expressing green fluorescent protein (GFP under the control of the stress-inducible HSP70B’ promoter were constructed. Exposure of HaCaT sensor cells to 25 µM cadmium, a model substance for oxidative stress induction, provoked a 1.7-fold increase in total glutathione and a ~300-fold induction of transcript level of the gene coding for heat shock protein HSP70B’. An extract of Arnica montana flowers resulted in a strong induction of the HSP70B’ gene and a pronounced decrease of total glutathione in keratinocytes. The HSP70B’ promoter-based sensor cells conveniently detected cadmium-induced stress using GFP fluorescence as read-out with a limit of detection of 6 µM cadmium. In addition the sensor cells responded to exposure of cells to A. montana extract with induction of GFP fluorescence. Thus, the HaCaT sensor cells provide a means for the automated detection of the compromised redox status of keratinocytes as an early indicator of the development of human skin disorders and could be applied for the prediction of skin irritation in more complex in vitro 3D human skin models and in the development of micro-total analysis systems (µTAS that may be utilized in dermatology, toxicology, pharmacology and drug screenings.

  9. TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines

    Directory of Open Access Journals (Sweden)

    C.A. Dalla Torre

    2002-01-01

    Full Text Available Measurement of telomerase activity in clinically obtained tumor samples may provide important information for use as both a diagnostic marker and a prognostic indicator for patient outcome. In order to evaluate telomerase activity in tumor tissue without radiolabeling the product, we developed a simple telomeric repeat amplification protocol-silver-staining assay that is less time-consuming, is safe and requires minimal equipment. In addition, we determined the sensitivity of the silver-staining method by using extracts of telomerase-positive thyroid carcinoma cell lines which were serially diluted from 5,000 to 10 cells. Telomerase activity was also assayed in 19 thyroid tumors, 2 normal controls and 27 bone marrow aspirates. The results indicate that the technique permits the detection of telomerase activity from 5000 to as few as 10 cells. We propose that it could be immediately applicable in many laboratories due to the minimal amount of equipment required.

  10. The α2C-adrenoceptor antagonist, ORM-10921, exerts antidepressant-like effects in the Flinders Sensitive Line rat.

    Science.gov (United States)

    Uys, Madeleine M; Shahid, Mohammed; Sallinen, Jukka; Harvey, Brian H

    2017-02-01

    Depression involves deficits in monoaminergic neurotransmission. Differential roles for α2A, B and C subtypes of the α2-adrenoceptor (AR) are evident, with selective α2C-AR antagonists purported to have antidepressant and procognitive properties. However, this has not been demonstrated in a genetic animal model of depression. The role of the α2C-AR in modulating two key depression-related behaviours in the Flinders Sensitive Line (FSL) rat was studied using a dose-response analysis following subcutaneous administration with the selective α2C-AR antagonist ORM-10921 (0.03; 0.3 mg/kg), the nonselective α2-AR antagonist idazoxan (3 mg/kg), or vehicle once daily for 14 days. Behaviour in the novel object recognition test, forced swim test (FST) and locomotor activity test was assessed. To ratify the validity of the FSL model, the reference tricyclic antidepressant imipramine (15 mg/kg, intraperitoneally) was used as a comparator drug in the FST. FSL rats demonstrated significantly increased immobility and recognition memory deficits versus Flinders Resistant Line controls, with imipramine significantly reversing said immobility. Similarly, ORM-10921 at both doses but not idazoxan significantly reversed immobility in the FST as well as attenuated cognitive deficits in FSL animals. We conclude that selective α2C-AR antagonism has potential as a novel therapeutic strategy in the treatment of depression and cognitive dysfunction.

  11. Sensitive optical detection of an early metastatic tumor using a new cell line with enhanced luminescent and fluorescent signals

    Directory of Open Access Journals (Sweden)

    Yeon Joo Kim

    2011-10-01

    Full Text Available Animal models using cell lines that are dual-labeled with luciferase and green fluorescent protein (GFP are powerful tools for performing simultaneous quantitative and qualitative analysis of metastatic tumors. However, the applications of such dual-labeled tumor models have been limited due to the technical challenges associated with low bioluminescent signaling from tumor cells. Here, we used lentiviral vector (LV encoding firefly luciferase and GFP and engineered a more sensitive and highly metastatic prostate cancer cell line (MLL-Luc/GFP cells, which allows simultaneous fluorescence and luminescence imaging. The light emission of MLL-Luc/GFP cells was 33.5 fold higher than that of PC-3-luc2-GFP prostate cancer cells which showed 750 p/s light emission per cell. Furthermore, the MLL-Luc/GFP cells showed 3.9 fold higher luciferase activities than did 4T1-luc2, which was previously recognized as exhibiting the highest luciferase activity. An in vivo evaluation with optical imaging showed pinpoint localization of GFP-positive cells in a metastatic lung as well as easy detection of early metastatic spreading. The newly engineered MLL-Luc/GFP cells provide an appropriate metastatic animal model system for future studies of metastasis and the testing of anti-metastatic therapies specifically aimed at prostatic cancer.

  12. Ribavirin restores ESR1 gene expression and tamoxifen sensitivity in ESR1 negative breast cancer cell lines

    Directory of Open Access Journals (Sweden)

    Sappok Anne

    2011-12-01

    Full Text Available Abstract Tumor growth is estrogen independent in approximately one-third of all breast cancers, which makes these patients unresponsive to hormonal treatment. This unresponsiveness to hormonal treatment may be explained through the absence of the estrogen receptor alpha (ESR1. The ESR1 gene re-expression through epigenetic modulators such as DNA methyltransferase inhibitors and/or histone deacetylase inhibitors restores tamoxifen sensitivity in ESR1 negative breast cancer cell lines and opens new treatment horizons in patients who were previously associated with a poor prognosis. In the study presented herein, we tested the ability of ribavirin, which shares some structural similarities with the DNA-methyltransferase inhibitor 5-azacytidine and which is widely known as an anti-viral agent in the treatment of hepatitis C, to restore ESR1 gene re-expression in ESR1 negative breast cancer cell lines. In our study we identified ribavirin to restore ESR1 gene re-expression alone and even more in combination with suberoylanilide hydroxamic acid (SAHA - up to 276 fold induction. Ribavirin and analogs could pave the way to novel translational research projects that aim to restore ESR1 gene re-expression and thus the susceptibility to tamoxifen-based endocrine treatment strategies.

  13. Comparative proteome analysis of drought-sensitive and drought-tolerant rapeseed roots and their hybrid F1 line under drought stress.

    Science.gov (United States)

    Mohammadi, Payam Pour; Moieni, Ahmad; Komatsu, Setsuko

    2012-11-01

    Rapeseed (Brassica napus L.), which is the third leading source of vegetable oil, is sensitive to drought stress during the early vegetative growth stage. To investigate the initial response of rapeseed to drought stress, changes in the protein expression profiles of drought-sensitive (RGS-003) and drought-tolerant lines (SLM-003), and their F1 hybrid, were analyzed using a proteomics approach. Seven-day-old rapeseed seedlings were treated with drought stress by restricting water for 7 days, and proteins were extracted from roots and separated by two-dimensional polyacrylamide gel electrophoresis. In the sensitive rapeseed line, 35 protein spots were differentially expressed under drought stress, and proteins related to metabolism, energy, disease/defense, and transport were decreased. In the tolerant line, 32 protein spots were differentially expressed under drought stress, and proteins involved in metabolism, disease/defense, and transport were increased, while energy-related proteins were decreased. Six protein spots in F1 hybrid were common among expressed proteins in the drought-sensitive and -tolerant lines. Notably, tubulin beta-2 and heat shock protein 70 were decreased in the drought-sensitive line and hybrid F1 plants, while jasmonate-inducible protein and 20S proteasome subunit PAF1 were increased in the F1 hybrids and drought-tolerant line. These results indicate that (1) V-type H(+) ATPase, plasma-membrane associated cation-binding protein, HSP 90, and elongation factor EF-2 have a role in the drought tolerance of rapeseed; (2) The decreased levels of heat shock protein 70 and tubulin beta-2 in the drought-sensitive and hybrid F1 lines might explain the reduced growth of these lines in drought conditions.

  14. Variation in drug sensitivity of malignant mesothelioma cell lines with substantial effects of selenite and bortezomib, highlights need for individualized therapy.

    Directory of Open Access Journals (Sweden)

    Adam Szulkin

    Full Text Available Malignant mesothelioma cells have an epithelioid or sarcomatoid morphology, both of which may be present in the same tumor. The sarcomatoid phenotype is associated with worse prognosis and heterogeneity of mesothelioma cells may contribute to therapy resistance, which is often seen in mesothelioma. This study aimed to investigate differences in sensitivity between mesothelioma cell lines to anti-cancer drugs. We studied two novel drugs, selenite and bortezomib and compared their effect to four conventional drugs. We also investigated the immunoreactivity of potential predictive markers for drug sensitivity; Pgp, MRP-1, ERCC1, RRM1, TS, xCT and proteasome 20S subunit.We treated six mesothelioma cell lines with selenite, bortezomib, carboplatin, pemetrexed, doxorubicin or gemcitabine as single agents and in combinations. Viability was measured after 24 and 48 hours. Immunocytochemistry was used to detect predictive markers.As a single agent, selenite was effective on four out of six cell lines, and in combination with bortezomib yielded the greatest response in the studied mesothelioma cell lines. Cells with an epithelioid phenotype were generally more sensitive to the different drugs than the sarcomatoid cells. Extensive S-phase arrest was seen in pemetrexed-sensitive cell lines. MRP-1 predicted sensitivity of cell lines to treatment with carboplatin and xCT predicted pemetrexed effect.The observed heterogeneity in sensitivity of mesothelioma cell lines with different morphology highlights the need for more individualized therapy, requiring development of methods to predict drug sensitivity of individual tumors. Selenite and bortezomib showed a superior effect compared to conventional drugs, motivating clinical testing of these agents as future treatment regime components for patients with malignant mesothelioma.

  15. Inorganic polyphosphate enhances radio-sensitivity in a human non-small cell lung cancer cell line, H1299.

    Science.gov (United States)

    Tsutsumi, Kaori; Matsuya, Yusuke; Sugahara, Tomoki; Tamura, Manami; Sawada, Satoshi; Fukura, Sagiri; Nakano, Hisashi; Date, Hiroyuki

    2017-06-01

    Inorganic polyphosphate is a linear polymer containing tens to hundreds of orthophosphate residues linked by high-energy phosphoanhydride bonds. Polyphosphate has been recognized as a potent anti-metastasis reagent. However, the molecular mechanism underlying polyphosphate action on cancer cells is poorly understood. In this study, we investigated the involvement of polyphosphate in radio-sensitivity using a human non-small cell lung cancer cell line, H1299. We found that polyphosphate treatment decreases cellular adenosine triphosphate levels, suggesting a disruption of energy metabolism. We also found that the induction of DNA double-strand breaks was enhanced in polyphosphate-treated cells after X-ray irradiation and colony formation assay revealed that cell survival decreased compared with that of the control groups. These findings suggest that polyphosphate is a promising radio-sensitizer for cancer cells. Therefore, we hypothesized that polyphosphate treatment disrupts adenosine triphosphate-mediated energy transfer for cellular survival and DNA repair, thereby reducing the cellular capability to resist X-ray irradiation.

  16. TOP1 gene copy numbers in colorectal cancer samples and cell lines and their association to in vitro drug sensitivity

    DEFF Research Database (Denmark)

    Rømer, Maria Unni; Jensen, Niels Frank; Nielsen, Signe Lykke

    2012-01-01

    Background and aims: A positive relationship between topoisomerase-1 (TOP1) protein and sensitivity towards the TOP1 inhibitor irinotecan has been reported in patients with metastatic colorectal cancer (mCRC). The aim of this study was to analyse TOP1 gene copy number variation in tumor tissue from...... and in vitro sensitivity to SN-38 (the active metabolite of irinotecan) and oxaliplatin were tested for 10 CRC cell lines. Results: The crude TOP1 copy numbers as well as the TOP1/CEN-20 mean ratio +/- 3 STD were determined in non-affected mucosa and in the malignant epithelium of the tumors. In the malignant...... epithelium 84% of the samples demonstrated an increased TOP1 gene copy number and 66% had an increased TOP1/CEN-20 ratio compared to the non-affected mucosa. Sixteen (32%) of the tumors had a ratio = 1.5 and 9 of these had a ratio of = 2.0. A positive association was observed between the TOP1 gene copy...

  17. Bioinformatic analyses identifies novel protein-coding pharmacogenomic markers associated with paclitaxel sensitivity in NCI60 cancer cell lines

    Directory of Open Access Journals (Sweden)

    Jarjanazi Hamdi

    2011-02-01

    Full Text Available Abstract Background Paclitaxel is a microtubule-stabilizing drug that has been commonly used in treating cancer. Due to genetic heterogeneity within patient populations, therapeutic response rates often vary. Here we used the NCI60 panel to identify SNPs associated with paclitaxel sensitivity. Using the panel's GI50 response data available from Developmental Therapeutics Program, cell lines were categorized as either sensitive or resistant. PLINK software was used to perform a genome-wide association analysis of the cellular response to paclitaxel with the panel's SNP-genotype data on the Affymetrix 125 k SNP array. FastSNP software helped predict each SNP's potential impact on their gene product. mRNA expression differences between sensitive and resistant cell lines was examined using data from BioGPS. Using Haploview software, we investigated for haplotypes that were more strongly associated with the cellular response to paclitaxel. Ingenuity Pathway Analysis software helped us understand how our identified genes may alter the cellular response to paclitaxel. Results 43 SNPs were found significantly associated (FDR CFTR, ROBO1, PTPRD, BTBD12, DCT, SNTG1, SGCD, LPHN2, GRIK1, ZNF607. SNPs in GRIK1, DCT, SGCD and CFTR were predicted to be intronic enhancers, altering gene expression, while SNPs in ZNF607 and BTBD12 cause conservative missense mutations. mRNA expression analysis supported these findings as GRIK1, DCT, SNTG1, SGCD and CFTR showed significantly (p GRIK1, SGCD, ROBO1, LPHN2, and PTPRD were more strongly associated with response than their individual SNPs. Conclusions Our study has taken advantage of available genotypic data and its integration with drug response data obtained from the NCI60 panel. We identified 10 SNPs located within protein-coding genes that were not previously shown to be associated with paclitaxel response. As only five genes showed differential mRNA expression, the remainder would not have been detected solely

  18. The Effects of Selected Sesquiterpenes from Myrica rubra Essential Oil on the Efficacy of Doxorubicin in Sensitive and Resistant Cancer Cell Lines

    Directory of Open Access Journals (Sweden)

    Martin Ambrož

    2017-06-01

    Full Text Available β-caryophyllene oxide (CAO, α-humulene (HUM, trans-nerolidol (NER and valencene (VAL are constituents of the essential oil of Myrica rubra (MEO, which has significant antiproliferative effect in various cancer cell lines. In the present study, we compared the antiproliferative effect of these sesquiterpenes alone and in combination with the cytostatic drug doxorubicin (DOX in cancer cell lines with different sensitivity to DOX. Two ovarian cancer cell lines (sensitive A2780 and partly resistant SKOV3 and two lymphoblast cancer cell lines (sensitive CCRF/CEM and completely resistant CEM/ADR were used. The observed effects varied among sesquiterpenes and also differed in individual cell lines, with only VAL being effective in all the cell lines. A strong synergism of DOX with NER was found in the A2780 cells, while DOX acted synergistically with HUM and CAO in the SKOV3 cells. In the CCRF/CEM cells, a synergism of DOX with CAO and NER was observed. In resistant CEM/ADR cells, sesquiterpenes did not increase DOX efficacy, although they significantly increased accumulation of DOX (up to 10-times and rhodamine-123 (substrate of efflux transporter ABCB1 within cancer cells. In conclusion, the tested sesquiterpenes were able to improve DOX efficacy in the sensitive and partly resistant cancer cells, but not in cells completely resistant to DOX.

  19. Probiotic treatment protects against the pro-depressant-like effect of high-fat diet in Flinders Sensitive Line rats.

    Science.gov (United States)

    Abildgaard, Anders; Elfving, Betina; Hokland, Marianne; Lund, Sten; Wegener, Gregers

    2017-10-01

    Major depressive disorder (MDD) is highly associated with dysmetabolic conditions, such as obesity and diabetes mellitus type 2, and the gut microbiota may interact with both disease entities. We have previously shown that a high-fat diet (HFD) exacerbated depressive-like behaviour uniquely in Flinders Sensitive Line (FSL) rats that inherently present with an increased level of depressive-like behaviour compared with Flinders Resistant Line (FRL) rats. We therefore investigated whether multispecies probiotics possessed anti-depressant-like effect in FSL rats or protected against the pro-depressant-like effect of HFD. We also examined blood and cerebral T cell subsets as well as plasma cytokines. Lastly, we investigated the effect of HFD in outbred Sprague-Dawley (SD) rats to substantiate the association between depressive-like behaviour and any immunological measures affected by HFD. HFD exacerbated the depressive-like behaviour in FSL rats in the forced swim test, whereas SD rats remained unaffected. Probiotic treatment completely precluded the pro-depressant-like effect of HFD, but it did not affect FSL rats on control diet. Cerebral T lymphocyte CD4/8 ratios closely mirrored the behavioural changes, whereas the proportions of Treg and Th17 subsets were unaltered. No association between blood and brain CD4/8 ratios were evident; nor did plasma cytokine levels change as a consequence of HFD of probiotic treatment. Our findings suggest that MDD may hold a dysmetabolic component that responds to probiotic treatment. This finding has wide implications owing to the high metabolic comorbidity in MDD. Furthermore, the close association between depressive-like behaviour and cerebral T cell populations demonstrate lymphocyte-brain interactions as a promising future research area in the field of psychoneuroimmunology. Copyright © 2017 Elsevier Inc. All rights reserved.

  20. Glutamine drives glutathione synthesis and contributes to radiation sensitivity of A549 and H460 lung cancer cell lines

    Science.gov (United States)

    Sappington, Daniel R.; Siegel, Eric R.; Hiatt, Gloria; Desai, Abhishek; Penney, Rosalind B.; Jamshidi-Parsian, Azemat; Griffin, Robert J.; Boysen, Gunnar

    2016-01-01

    Background Increased glutamine uptake is known to drive cancer cell proliferation, making tumor cells glutamine-dependent. Glutamine provides additional carbon and nitrogen sources for cell growth. The first step in glutamine utilization is its conversion to glutamate by glutaminase (GLS). Glutamate is a precursor for glutathione synthesis, and we investigated the hypothesis that glutamine drives glutathione synthesis and thereby contributes to cellular defense systems. Methods The importance of glutamine for glutathione synthesis was studied in H460 and A549 lung cancer cell lines using glutamine-free medium and Bis-2-(5-phenyl-acetamido-1,3,4-thiadiazol-2-yl)ethyl sulfide (BPTES) a GLS inhibitor. Metabolic activities were determined by targeted mass spectrometry. Results A significant correlation between glutamine consumption and glutathione excretion was demonstrated in H460 and A549 tumor cells. Culturing in the presence of [13C5]glutamine demonstrated that by 12 hrs >50% of excreted glutathione is derived from glutamine. Culturing in glutamine-free medium or treatment with BPTES, a glutaminase (GLS)-specific inhibitor, reduced cell proliferation and viability, and abolished glutathione excretion. Treatment with glutathione-ester prevented BPTES induced cytotoxicity. Inhibition of GLS markedly radiosensitized the lung tumor cell lines, suggesting an important role of glutamine-derived glutathione in determining radiation sensitivity. Conclusions We demonstrate here for the first time that a significant amount of extracellular glutathione is directly derived from glutamine. This finding adds yet another important function to the already known glutamine dependence of tumor cells and probably tumors as well. General significance Glutamine is essential for synthesis and excretion of glutathione to promote cell growth and viability. PMID:26825773

  1. The brain 5-HT4 receptor binding is down-regulated in the Flinders Sensitive Line depression model and in response to paroxetine administration

    DEFF Research Database (Denmark)

    Licht, Cecilie Löe; Marcussen, Anders Bue; Wegener, Gregers

    2009-01-01

    The 5-hydroxytryptamine (5-HT(4)) receptor may be implicated in depression and is a new potential target for antidepressant treatment. We have investigated the brain 5-HT(4) receptor [(3)H]SB207145 binding in the Flinders Sensitive Line rat depression model by quantitative receptor autoradiography...... cortices after chronic paroxetine administration, and markedly reduced in several regions after 5-HT depletion. Thus, the 5-HT(4) receptor binding was decreased in the Flinders Sensitive Line depression model and in response to chronic paroxetine administration....

  2. Influence of Mesenchymal Stem Cells Conditioned Media on Proliferation of Urinary Tract Cancer Cell Lines and Their Sensitivity to Ciprofloxacin.

    Science.gov (United States)

    Maj, Malgorzata; Bajek, Anna; Nalejska, Ewelina; Porowinska, Dorota; Kloskowski, Tomasz; Gackowska, Lidia; Drewa, Tomasz

    2017-06-01

    Mesenchymal stem cells (MSCs) are known to interact with cancer cells through direct cell-to-cell contact and secretion of paracrine factors, although their exact influence on tumor progression in vivo remains unclear. To better understand how fetal and adult stem cells affect tumors, we analyzed viability of human renal (786-0) and bladder (T24) carcinoma cell lines cultured in conditioned media harvested from amniotic fluid-derived stem cells (AFSCs) and adipose-derived stem cells (ASCs). Both media reduced metabolic activity of 786-0 cells, however, decreased viability of T24 cells was noted only after incubation with conditioned medium from ASCs. To test the hypothesis that MSCs-secreted factors might be involved in chemoresistance acquisition, we further analyzed influence of mesenchymal stem cell conditioned media (MSC-CM) on cancer cells sensitivity to ciprofloxacin, that is considered as potential candidate agent for urinary tract cancers treatment. Significantly increased resistance to tested drug indicates that MSCs may protect cancer cells from chemotherapy. J. Cell. Biochem. 118: 1361-1368, 2017. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  3. New beam line for time-of-flight medium energy ion scattering with large area position sensitive detector.

    Science.gov (United States)

    Linnarsson, M K; Hallén, A; Åström, J; Primetzhofer, D; Legendre, S; Possnert, G

    2012-09-01

    A new beam line for medium energy ion mass scattering (MEIS) has been designed and set up at the Ångström laboratory, Uppsala University, Sweden. This MEIS system is based on a time-of-flight (ToF) concept and the electronics for beam chopping relies on a 4 MHz function generator. Repetition rates can be varied between 1 MHz and 63 kHz and pulse widths below 1 ns are typically obtained by including beam bunching. A 6-axis goniometer is used at the target station. Scattering angle and energy of backscattered ions are extracted from a time-resolved and position-sensitive detector. Examples of the performance are given for three kinds of probing ions, (1)H(+), (4)He(+), and (11)B(+). Depth resolution is in the nanometer range and 1 and 2 nm thick Pt layers can easily be resolved. Mass resolution between nearby isotopes can be obtained as illustrated by Ga isotopes in GaAs. Taking advantage of the large size detector, a direct imaging (blocking pattern) of crystal channels are shown for hexagonal, 4H-SiC. The ToF-MEIS system described in this paper is intended for use in semiconductor and thin film areas. For example, depth profiling in the sub nanometer range for device development of contacts and dielectric interfaces. In addition to applied projects, fundamental studies of stopping cross sections in this medium energy range will also be conducted.

  4. Altered local field potential activity and serotonergic neurotransmission are further characteristics of the Flinders sensitive line rat model of depression.

    Science.gov (United States)

    Voget, Mareike; Rummel, Julia; Avchalumov, Yosef; Sohr, Reinhard; Haumesser, Jens K; Rea, Ellis; Mathé, Aleksander A; Hadar, Ravit; van Riesen, Christoph; Winter, Christine

    2015-09-15

    A significant portion of patients suffering from major depression remains refractory to available antidepressant treatment strategies. This highlights the need for a better understanding of the underlying neuropathology in order to develop rationale-based treatments. Here we aimed to further characterize neurobiological abnormalities of the Flinders Sensitive Line (FSL) rat model of depression. Biochemically, in FSL rats we mainly found increased levels of serotonin in most cortical and subcortical brain regions when compared to controls. Using electrophysiological measurements, in FSL rats we found decreased alpha, beta and low gamma oscillatory activity in the medial prefrontal cortex and nucleus accumbens and decreased alpha and beta as well as increased low gamma oscillatory activity in the subthalamicus nucleus when compared to controls. In summary, we show distinct neurochemical properties in combination with particular oscillatory activity patterns for brain areas thought to be pathophysiologically relevant for depression. Our data contribute to the further understanding of neurobiological alterations in the FSL rat model of depression that could provide a basis for research into future therapeutic strategies. Copyright © 2015 Elsevier B.V. All rights reserved.

  5. Improving Blast Resistance of a Thermo-Sensitive Genic Male Sterile Rice Line GD-8S by Molecular Marker-Assisted Selection

    Directory of Open Access Journals (Sweden)

    Wu-ge LIU

    2008-09-01

    Full Text Available The broad-spectrum blast resistance gene Pi-1, from donor line BL122, was introduced into a thermo-sensitive genic male sterile rice line GD-8S, which possessed good grain quality but high susceptibility to rice blast, by using backcross breeding and molecular marker-assisted selection. Five elite improved male sterile lines, RGD8S-1, RGD8S-2, RGD8S-3, RGD8S-4 and RGD8S-5, were selected based on the results of molecular marker analysis, spikelet sterility, recovery rate of genetic background and agronomic traits. Thirty-three representative blast isolates collected from Guangdong Province, China were used to inoculate the improved lines and the original line GD-8S artificially. The resistance frequencies of the improved lines ranged from 76.47% to 100%, much higher than that of the original line GD-8S (9.09%. On the agronomic characters, there were no significant differences between the improved lines and GD-8S except for flag leaf length and panicle number per plant. The improved lines could be used for breeding hybrid rice with high blast resistance.

  6. From superWIMPs to decaying dark matter. Models, bounds and indirect searches

    Energy Technology Data Exchange (ETDEWEB)

    Weniger, Christoph

    2010-06-15

    Despite lots of observational and theoretical efforts, the particle nature of dark matter remains unknown. Beyond the paradigmatic WIMPs (Weakly Interacting Massive Particles), many theoretically well motivated models exist where dark matter interacts much more weakly than electroweak with Standard Model particles. In this case new phenomena occur, like the decay of dark matter or the interference with the standard cosmology of the early Universe. In this thesis we study some of these aspects of superweakly coupled dark matter in general, and in the special case of hidden U(1){sub X} gauginos that kinetically mix with hypercharge. There, we will assume that the gauge group remains unbroken, similar to the Standard Model U(1){sub em}. We study different kinds of cosmological bounds, including bounds from thermal overproduction, from primordial nucleosynthesis and from structure formation. Furthermore, we study the possible cosmic-ray signatures predicted by this scenario, with emphasis on the electron and positron channel in light of the recent observations by PAMELA and Fermi LAT. Moreover we study the cosmic-ray signatures of decaying dark matter independently of concrete particle-physics models. In particular we analyze in how far the rise in the positron fraction above 10 GeV, as observed by PAMELA, can be explained by dark matter decay. Lastly, we concentrate on related predictions for gamma-ray observations with the Fermi LAT, and propose to use the dipole-like anisotropy of the prompt gamma-ray dark matter signal to distinguish exotic dark matter contributions from the extragalactic gamma-ray background. (orig.)

  7. Effect of 41 degrees C and 43 degrees C on cisplatin radiosensitization in two human carcinoma cell lines with different sensitivities for cisplatin

    NARCIS (Netherlands)

    Bergs, Judith W. J.; Haveman, Jaap; ten Cate, Rosemarie; Medema, Jan Paul; Franken, Nicolaas A. P.; van Bree, Chris

    2007-01-01

    The effect of trimodality treatment consisting of hyperthermia, cisplatin and radiation was investigated in two cell lines with different sensitivities to cisplatin. Hyperthermia treatment was performed for 1 h at 41 degrees C and 43 degrees C in order to compare the effects of the two temperatures.

  8. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Northwest Arctic, Alaska: ESI (Environmental Sensitivity Index Shoreline Types - Polygons and Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing the shoreline and coastal habitats of Northwest Arctic, Alaska classified according to the...

  9. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Puget Sound and Strait of Juan de Fuca, Washington: ESI (Environmental Sensitivity Index Shoreline Types - Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing the shoreline and coastal habitats in Puget Sound and Strait of Juan de Fuca, Washington, classified...

  10. Trichostatin A (TSA) sensitizes the human prostatic cancer cell line DU145 to death receptor ligands treatment.

    Science.gov (United States)

    Taghiyev, Agshin F; Guseva, Natalya V; Sturm, Mary T; Rokhlin, Oskar W; Cohen, Michael B

    2005-04-01

    The human prostatic carcinoma cell line DU145 has previously been found to be resistant to treatment with TNF-family ligands. However, TRAIL, TNF-alpha and anti-Fas antibodies (Ab) treatment in combination with the histone deacetylase inhibitor Trichostatin A (TSA) converted the phenotype of DU145 from resistant to sensitive. TSA induced 15% cell death but simultaneous treatment with TRAIL, TNF-alpha and anti-Fas Ab resulted in 55%, 70% and 40% cell death, respectively. Simultaneous treatment did not increase the level of TSA-induced histone acetylation, but induced the release of acetylated histones from chromatin into the cytosol. This release was caspase dependent since it was abrogated by Z-VAD-fmk. In addition, treatment with TSA induced caspase-9 activation and resulted in the release of cytochrome c and Smac/DIABLO from mitochondria. To further investigate the role of caspase-9 in TSA-mediated apoptosis we used two different approaches: (1) cells were pretreated with the caspase-9 inhibitor Z-LEHD-fmk, and (2) cells were transfected with a dominant-negative form of caspase-9. Both approaches gave similar results: cells became resistant to treatment with TSA. These data indicate that TSA mediates its effect via the mitochondrial pathway. This was confirmed by examining DU145 overexpressing Bcl-2. These transfectants were resistant to TSA treatment. Taken together, our data shows that only simultaneous treatment with TNF-family ligands and TSA in DU145 resulted in caspase activity sufficient to induce apoptosis. The combination of TSA and TNF-family ligands could potentially be the basis for the treatment of prostate cancer.

  11. Differential sensitivity of cerebellar purkinje neurons to ethanol in selectively outbred lines of mice: maintenance in vitro independent of synaptic transmission.

    Science.gov (United States)

    Basile, A; Hoffer, B; Dunwiddie, T

    1983-03-28

    The effects of ethanol on spontaneous firing of cerebellar Purkinje neurons were examined in outbred lines of mice (short-sleep, SS; and long-sleep, LS) which exhibit differential behavioral sensitivity to ethanol. In order to determine whether the differences in Purkinje cell ethanol sensitivity which are observed in situ reflect differences in intrinsic properties of Purkinje neurons, we developed an isolated in vitro preparation of mouse cerebellum. Even when synaptic transmission was largely inhibited by elevating Mg2+ and decreasing Ca2+ concentrations, Purkinje cells demonstrated stable long-term firing rates quite similar to those observed in vivo. Purkinje cells responded to superfusion of ethanol with both increases and decreases in firing rate. Inhibition of rate was more commonly observed, and was the only response which was demonstrably dose-dependent. The differential sensitivity to ethanol which we have previously reported in vivo was maintained even under under these conditions, with the LS mice being approximately 5 times more sensitive to the depressant effects of ethanol. In addition, it was shown that ethanol, at the concentrations used in these experiments, decreased the amplitude and increased the duration of single action potentials. Thus, taken together, these results suggest that the differential sensitivity of outbred lines to the soporific effects of ethanol are paralleled by differences in the sensitivity of Purkinje neurons in vitro to superfusion with ethanol. Because these differences can be observed even when synaptic transmission is largely suppressed, it would appear that these differences are intrinsic to the purkinje neurons themselves.

  12. THE MAGNETIC SENSITIVITY OF THE Mg ii k LINE TO THE JOINT ACTION OF HANLE, ZEEMAN, AND MAGNETO-OPTICAL EFFECTS

    Energy Technology Data Exchange (ETDEWEB)

    Ballester, E. Alsina; Bueno, J. Trujillo [Instituto de Astrofísica de Canarias, E-38205 La Laguna, Tenerife (Spain); Belluzzi, L., E-mail: ealsina@iac.es, E-mail: jtb@iac.es, E-mail: belluzzi@irsol.ch [Istituto Ricerche Solari Locarno, CH-6605 Locarno Monti (Switzerland)

    2016-11-10

    We highlight the main results of a radiative transfer investigation on the magnetic sensitivity of the solar Mg ii k resonance line at 2795.5 Å, accounting for the joint action of the Hanle and Zeeman effects as well as partial frequency redistribution phenomena. We confirm that at the line center, the linear polarization signals produced by scattering processes are measurable, and that they are sensitive, via the Hanle effect, to magnetic fields with strengths between 5 and 50 G, approximately. We also show that the Zeeman effect produces conspicuous circular polarization signals, especially for longitudinal fields stronger than 50 G, which can be used to estimate the magnetization of the solar chromosphere via the familiar magnetograph formula. The most novel result is that magneto-optical effects produce, in the wings of the line, a decrease of the Q / I scattering polarization pattern and the appearance of U / I signals (i.e., a rotation of the plane of linear polarization). This sensitivity of the Q / I and U / I wing signals to both weak (∼5 G) and stronger magnetic fields expands the scientific interest of the Mg ii k line for probing the chromosphere in quiet and active regions of the Sun.

  13. Higher levels of c-Met expression and phosphorylation identify cell lines with increased sensitivity to AMG-458, a novel selective c-Met inhibitor with radiosensitizing effects.

    Science.gov (United States)

    Li, Bo; Torossian, Artour; Sun, Yunguang; Du, Ruihong; Dicker, Adam P; Lu, Bo

    2012-11-15

    c-Met is overexpressed in some non-small cell lung cancer (NSCLC) cell lines and tissues. Cell lines with higher levels of c-Met expression and phosphorylation depend on this receptor for survival. We studied the effects of AMG-458 on 2 NSCLC cell lines. 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assays assessed the sensitivities of the cells to AMG-458. Clonogenic survival assays illustrated the radiosensitizing effects of AMG-458. Western blot for cleaved caspase 3 measured apoptosis. Immunoblotting for c-Met, phospho-Met (p-Met), Akt/p-Akt, and Erk/p-Erk was performed to observe downstream signaling. AMG-458 enhanced radiosensitivity in H441 but not in A549. H441 showed constitutive phosphorylation of c-Met. A549 expressed low levels of c-Met, which were phosphorylated only in the presence of exogenous hepatocyte growth factor. The combination of radiation therapy and AMG-458 treatment was found to synergistically increase apoptosis in the H441 cell line but not in A549. Radiation therapy, AMG-458, and combination treatment were found to reduce p-Akt and p-Erk levels in H441 but not in A549. H441 became less sensitive to AMG-458 after small interfering RNA knockdown of c-Met; there was no change in A549. After overexpression of c-Met, A549 became more sensitive, while H441 became less sensitive to AMG-458. AMG-458 was more effective in cells that expressed higher levels of c-Met/p-Met, suggesting that higher levels of c-Met and p-Met in NSCLC tissue may classify a subset of tumors that are more sensitive to molecular therapies against this receptor. Copyright © 2012 Elsevier Inc. All rights reserved.

  14. Higher Levels of c-Met Expression and Phosphorylation Identify Cell Lines With Increased Sensitivity to AMG-458, a Novel Selective c-Met Inhibitor With Radiosensitizing Effects

    Energy Technology Data Exchange (ETDEWEB)

    Li Bo; Torossian, Artour [Department of Radiation Oncology, Vanderbilt-Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee (United States); Sun, Yunguang [Department of Radiation Oncology, Kimmel Cancer Center, Jefferson Medical College of Thomas Jefferson University, Philadelphia, Pennsylvania (United States); Du, Ruihong [Department of Radiation Oncology, Vanderbilt-Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee (United States); Dicker, Adam P. [Department of Radiation Oncology, Kimmel Cancer Center, Jefferson Medical College of Thomas Jefferson University, Philadelphia, Pennsylvania (United States); Lu Bo, E-mail: bo.lu@jefferson.edu [Department of Radiation Oncology, Vanderbilt-Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee (United States); Department of Radiation Oncology, Kimmel Cancer Center, Jefferson Medical College of Thomas Jefferson University, Philadelphia, Pennsylvania (United States)

    2012-11-15

    Purpose: c-Met is overexpressed in some non-small cell lung cancer (NSCLC) cell lines and tissues. Cell lines with higher levels of c-Met expression and phosphorylation depend on this receptor for survival. We studied the effects of AMG-458 on 2 NSCLC cell lines. Methods and Materials: 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl) -2H-tetrazolium assays assessed the sensitivities of the cells to AMG-458. Clonogenic survival assays illustrated the radiosensitizing effects of AMG-458. Western blot for cleaved caspase 3 measured apoptosis. Immunoblotting for c-Met, phospho-Met (p-Met), Akt/p-Akt, and Erk/p-Erk was performed to observe downstream signaling. Results: AMG-458 enhanced radiosensitivity in H441 but not in A549. H441 showed constitutive phosphorylation of c-Met. A549 expressed low levels of c-Met, which were phosphorylated only in the presence of exogenous hepatocyte growth factor. The combination of radiation therapy and AMG-458 treatment was found to synergistically increase apoptosis in the H441 cell line but not in A549. Radiation therapy, AMG-458, and combination treatment were found to reduce p-Akt and p-Erk levels in H441 but not in A549. H441 became less sensitive to AMG-458 after small interfering RNA knockdown of c-Met; there was no change in A549. After overexpression of c-Met, A549 became more sensitive, while H441 became less sensitive to AMG-458. Conclusions: AMG-458 was more effective in cells that expressed higher levels of c-Met/p-Met, suggesting that higher levels of c-Met and p-Met in NSCLC tissue may classify a subset of tumors that are more sensitive to molecular therapies against this receptor.

  15. Rhode Island, Connecticut, New York, and New Jersey ESI: ESI (Environmental Sensitivity Index Shoreline Types - Polygons and Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing the shoreline and coastal habitats of Rhode Island, Connecticut, New York, and New Jersey, classified...

  16. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Northwest Arctic, Alaska: SOCECON (Socioeconomic Resource Points and Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector points and lines representing human-use resource data for airports, marinas, and mining sites in Northwest Arctic, Alaska....

  17. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: North Carolina: ESI (ESI Shoreline Types - Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing the shoreline and coastal habitats of North Carolina classified according to the Environmental...

  18. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Southern California: ESI (Shoreline Types - Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing the shoreline and coastal habitats of Southern California, classified according to the Environmental...

  19. Calculations of H2O microwave line broadening in collisions with He atoms - Sensitivity to potential energy surfaces

    Science.gov (United States)

    Green, Sheldon; Defrees, D. J.; Mclean, A. D.

    1991-01-01

    Theoretical computations of broadening parameters are reported for three microwave lines of H2O in a bath of He atoms. The potential-energy surfaces employed are corrected for basis-set superposition error, and their reliability is checked by repeating the calculations with a different basis set for orbital expansion. The results are presented in extensive tables and discussed in detail. The corrections applied are shown to have a significant impact on the accuracy of the room-temperature broadenings determined: 8.9 sq A for the 22.2-GHz line, 11.8 sq A for the 183,3-GHz line, and 10.0 sq A for the 380.2-GHz line, in good agreement with published experimental data. The importance of collisional broadening for the atmospheric transmission of radiation and for remote-sensing applications is indicated.

  20. Insulin sensitivity and beta-cell function in the offspring of type 2 diabetic patients: impact of line of inheritance.

    LENUS (Irish Health Repository)

    Natali, Andrea

    2010-10-01

    What defects in glucose metabolism are present in offspring of type 2 diabetic patients (FHD(+) with a positive family history of diabetes) and to what extent they depend on line of inheritance are uncertain.

  1. In-line phase-sensitive amplification of QPSK signal using multiple quasi-phase matched LiNbO₃ waveguide.

    Science.gov (United States)

    Asobe, Masaki; Umeki, Takeshi; Takenouchi, Hirokazu; Miyamoto, Yutaka

    2014-11-03

    Phase-sensitive amplifiers (PSA) using periodically poled (PPLN) LiNbO₃ waveguides are promising as low-noise optical amplifiers. However, it is difficult to realize in-line operation for multi-level phase modulated signals using a PPLN based PSA with the conventional configuration. In this paper, we report a PPLN based in-line PSA that can regenerate quadrature phase shift keying (QPSK) signals. Multi-stage frequency mixing in a multiple quasi-phase matched LiNbO₃waveguide allows carrier phase recovery from a QPSK signal. Non-degenerate parametric amplification enables the phase-sensitive amplification of a QPSK signal. Amplitude and phase regeneration is examined utilizing gain saturation and phase squeezing capability.

  2. Hormone resistance in two MCF-7 breast cancer cell lines is associated with reduced mTOR signaling, decreased glycolysis and increased sensitivity to cytotoxic drugs

    Directory of Open Access Journals (Sweden)

    Euphemia Yee Leung

    2014-09-01

    Full Text Available The mTOR pathway is a key regulator of multiple cellular signaling pathways and is a potential target for therapy. We have previously developed two hormone-resistant sub-lines of the MCF-7 human breast cancer line, designated TamC3 and TamR3, which were characterized by reduced mTOR signaling, reduced cell volume and resistance to mTOR inhibition. Here we show that these lines exhibit increased sensitivity to carboplatin, oxaliplatin, 5-fluorouracil, camptothecin, doxorubicin, paclitaxel, docetaxel and hydrogen peroxide. The mechanisms underlying these changes have not yet been characterized but may include a shift from glycolysis to mitochondrial respiration. If this phenotype is found in clinical hormone-resistant breast cancers, conventional cytotoxic therapy may be a preferred option for treatment.

  3. Mechanisms underlying radiosensitivity : iIvestigations in xrs-5, an X-ray sensitive hamster cell line

    Science.gov (United States)

    Johnston, Peter James

    The damage caused to cells by ionising radiation is believed to center on damage to the DNA. In particular, the induction of DNA double strand breaks (DSB) have been implicated in biological end-points such as cell killing and the formation of chromosomal aberrations. The xrs-5 cell line is a mutant Chinese hamster ovary fibroblast (CHO-K1) mutant which exhibits sensitivity to ionising radiation and a number of other DNA damaging agents. This mutation, postulated to involve the hamster homologue of the human XRCC5 gene, is believed to be involved in the repair of the DSB. In addition, there are constitutive differences between the wild type and xrs cells involving the structure and function of the nucleus and higher order chromatin structures. The aims of this thesis were to study further the xrs-5 cell line and its response to DNA damage and to investigate the possible link between chromatin structure and DSB repair. By the examination of the response of xrs-5 cells to a number of DNA damaging agents and potential modulators of this response using the cytokinesis block micronucleus assay [Fenech and Morley, 1985] a possible cell cycle defect was identified in addition to elevated levels of chromosomal damage. Xrs-5 cells appeared to be partially defective in the cell cycle checkpoints involving the passage from G2 phase to mitosis. By the use of a modified neutral filter elution procedure variations in the repair of DSB were observed between xrs-5 and CHO. Conventional neutral filter elution requires harsh lysis conditions to remove higher order chromatin structures which interfere with the elution of DNA containing DSB. By lysing cells with non-ionic detergent in the presence of 2 M NaC1, histone depleted structures which retain the higher order nuclear matrix organisation, including chromatin loops, can be produced. Elution from these structures will only occur if two or more DSB lie within a single looped domain delineated by points of attachment to the nuclear

  4. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Northwest Arctic, Alaska: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector arcs and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for Northwest...

  5. Genetic variants in urinary bladder cancer: collective power of the "wimp SNPs".

    Science.gov (United States)

    Golka, Klaus; Selinski, Silvia; Lehmann, Marie-Louise; Blaszkewicz, Meinolf; Marchan, Rosemarie; Ickstadt, Katja; Schwender, Holger; Bolt, Hermann M; Hengstler, Jan G

    2011-06-01

    In recent years, genome-wide association studies (GWAS) have identified more than 300 validated associations between genetic variants and risk of approximately 70 common diseases. A small number of rare variants with a frequency of usually less than 1% are associated with a strongly enhanced risk, such as genetic variants of TP53, RB1, BRCA1, and BRCA2. Only a very small number of SNPs (with a frequency of more that 1% of the rare allele) have effects of a factor of two or higher. Examples include APOE4 in Alzheimer's disease, LOXL1 in exfoliative glaucoma, and CFH in age-related macular degeneration. However, the majority of all identified SNPs have odds ratios between 1.1 and 1.5. In the case of urinary bladder cancer, all known SNPs that have been validated in sufficiently large populations are associated with odds ratios smaller than 1.5. These SNPs are located next to the following genes: MYC, TP63, PSCA, the TERT-CLPTM1L locus, FGFR3, TACC3, NAT2, CBX6, APOBEC3A, CCNE1, and UGT1A. It is likely that these moderate risk or "wimp SNPs" interact, and because of their high number, collectively have a strong influence on whether an individual will develop cancer or not. It should be considered that variants identified so far explain only approximately 5-10% of the overall inherited risk. Possibly, the remaining variance is due to an even higher number of SNPs with odds ratios smaller than 1.1. Recent studies have provided the following information: (1) The functions of genes identified as relevant for bladder cancer focus on detoxification of carcinogens, control of the cell cycle and apoptosis, as well as maintenance of DNA integrity. (2) Many novel SNPs are far away from the protein coding regions, suggesting that these SNPs are located on distant-acting transcriptional enhancers. (3) The low odds ratio of each individual bladder cancer-associated SNP is too low to justify reasonable preventive measures. However, if the recently identified SNPs interact, they may

  6. Gene expression patterns that predict sensitivity to epidermal growth factor receptor tyrosine kinase inhibitors in lung cancer cell lines and human lung tumors

    Directory of Open Access Journals (Sweden)

    Haura Eric B

    2006-11-01

    Full Text Available Abstract Background Increased focus surrounds identifying patients with advanced non-small cell lung cancer (NSCLC who will benefit from treatment with epidermal growth factor receptor (EGFR tyrosine kinase inhibitors (TKI. EGFR mutation, gene copy number, coexpression of ErbB proteins and ligands, and epithelial to mesenchymal transition markers all correlate with EGFR TKI sensitivity, and while prediction of sensitivity using any one of the markers does identify responders, individual markers do not encompass all potential responders due to high levels of inter-patient and inter-tumor variability. We hypothesized that a multivariate predictor of EGFR TKI sensitivity based on gene expression data would offer a clinically useful method of accounting for the increased variability inherent in predicting response to EGFR TKI and for elucidation of mechanisms of aberrant EGFR signalling. Furthermore, we anticipated that this methodology would result in improved predictions compared to single parameters alone both in vitro and in vivo. Results Gene expression data derived from cell lines that demonstrate differential sensitivity to EGFR TKI, such as erlotinib, were used to generate models for a priori prediction of response. The gene expression signature of EGFR TKI sensitivity displays significant biological relevance in lung cancer biology in that pertinent signalling molecules and downstream effector molecules are present in the signature. Diagonal linear discriminant analysis using this gene signature was highly effective in classifying out-of-sample cancer cell lines by sensitivity to EGFR inhibition, and was more accurate than classifying by mutational status alone. Using the same predictor, we classified human lung adenocarcinomas and captured the majority of tumors with high levels of EGFR activation as well as those harbouring activating mutations in the kinase domain. We have demonstrated that predictive models of EGFR TKI sensitivity can

  7. Integrated cantilever-based flow sensors with tunable sensitivity for in-line monitoring of flow fluctuations in microfluidic systems

    DEFF Research Database (Denmark)

    Noeth, Nadine-Nicole; Keller, Stephan Sylvest; Boisen, Anja

    2014-01-01

    For devices such as bio-/chemical sensors in microfluidic systems, flow fluctuations result in noise in the sensor output. Here, we demonstrate in-line monitoring of flow fluctuations with a cantilever-like sensor integrated in a microfluidic channel. The cantilevers are fabricated in different m...... pumps connected to the microfluidic system. © 2013 by the authors; licensee MDPI, Basel, Switzerland....

  8. Sensitivity and resistance towards isoliquiritigenin, doxorubicin and methotrexate in T cell acute lymphoblastic leukaemia cell lines by pharmacogenomics.

    Science.gov (United States)

    Youns, Mahmoud; Fu, Yu-Jie; Zu, Yuan-Gang; Kramer, Anne; Konkimalla, V Badireenath; Radlwimmer, Bernhard; Sültmann, Holger; Efferth, Thomas

    2010-09-01

    The development of drug resistance in cancer cells necessitates the identification of novel agents with improved activity towards cancer cells. In the present investigation, we compared the cytotoxicity of the chalcone flavonoide, isoliquiritigenin (ISL), with that of doxorubicin (DOX) and methotrexate (MTX) in five T cell acute lymphoblastic leukaemia (T-ALL) cell lines (Jurkat, J-Jhan, J16, HUT78 and Karpas 45). To gain insight into the molecular mechanisms which determine the response of T-ALL cells towards ISL, DOX and MTX, we applied array-based matrix comparative genomic hybridisation and microarray-based mRNA expression profiling and compared the genomic and transcriptomic profiles of the cell lines with their 50% inhibition (IC(50)) values for these three drugs. The IC(50) values for ISL did not correlate with those for DOX or MTX, indicating that ISL was still active in DOX- or MTX-unresponsive cell lines. Likewise, the genomic imbalances of chromosomal clones and mRNA expression profile significantly correlating with IC(50) values for ISL were different from thoses correlating with IC(50) values for DOX and MTX. In conclusion, ISL represents a cytotoxic natural product with activity towards T-ALL cell lines. There was no cross-resistance between ISL and DOX or MTX, and the genomic and transcriptomic profiles pointed to different molecular modes of action of ISL as compared to DOX and MTX, indicating that ISL may be a valuable adjunct for cancer therapy to treat otherwise drug-resistant tumours.

  9. hMena+11a Isoform Serves as a Marker of Epithelial Phenotype and Sensitivity to EGFR Inhibition in Human Pancreatic Cancer Cell Lines

    Science.gov (United States)

    Pino, Maria S.; Balsamo, Michele; Di Modugno, Francesca; Mottolese, Marcella; Alessio, Massimo; Melucci, Elisa; Milella, Michele; McConkey, David J.; Philippar, Ulrike; Gertler, Frank B.; Natali, Pier Giorgio; Nistico, Paola

    2008-01-01

    Purpose hMena, member of the Ena/VASP protein family, is a cytoskeletal protein that is involved in the regulation of cell motility and adhesion. The aim of this study was to determine whether or not the expression of hMena isoforms correlated with sensitivity to EGFR tyrosine kinase inhibitors and could serve as markers with potential clinical use. Experimental design Human pancreatic ductal adenocarcinoma (PDAC) cell lines were characterized for in vitro sensitivity to erlotinib, expression of HER family receptors, markers of epithelial to mesenchymal transition (EMT), and expression of hMena and its isoform hMena+11a. The effects of EGF and erlotinib on hMena expression as well as the effect of hMena knock-down on cell proliferation were also evaluated. Results hMena was detected in all of the pancreatic tumor cell lines tested as well as in the majority of the human tumor samples [primary (92%) and metastatic (86%)]. Intriguingly, in vitro hMena+11a isoform was specifically associated with an epithelial phenotype, EGFR dependency and sensitivity to erlotinib. In epithelial BxPC3 cells EGF upregulated hMena/hMena+11a and erlotinib downregulated expression. hMena knock-down reduced cell proliferation and MAPK and AKT activation in BxPC3 cells and promoted the growth inhibitory effects of erlotinib. Conclusions Collectively, our data indicate that the hMena+11a isoform is associated with an epithelial phenotype and identifies EGFR dependent cell lines that are sensitive to the EGFR inhibitor erlotinib. The availability of anti-hMena+11a specific probes may offer a new tool in pancreatic cancer management if these results can be verified prospectively in cancer patients. PMID:18676769

  10. Human Mena+11a isoform serves as a marker of epithelial phenotype and sensitivity to epidermal growth factor receptor inhibition in human pancreatic cancer cell lines.

    Science.gov (United States)

    Pino, Maria S; Balsamo, Michele; Di Modugno, Francesca; Mottolese, Marcella; Alessio, Massimo; Melucci, Elisa; Milella, Michele; McConkey, David J; Philippar, Ulrike; Gertler, Frank B; Natali, Pier Giorgio; Nisticò, Paola

    2008-08-01

    hMena, member of the enabled/vasodilator-stimulated phosphoprotein family, is a cytoskeletal protein that is involved in the regulation of cell motility and adhesion. The aim of this study was to determine whether or not the expression of hMena isoforms correlated with sensitivity to EGFR tyrosine kinase inhibitors and could serve as markers with potential clinical use. Human pancreatic ductal adenocarcinoma cell lines were characterized for in vitro sensitivity to erlotinib, expression of HER family receptors, markers of epithelial to mesenchymal transition, and expression of hMena and its isoform hMena(+11a). The effects of epidermal growth factor (EGF) and erlotinib on hMena expression as well as the effect of hMena knockdown on cell proliferation were also evaluated. hMena was detected in all of the pancreatic tumor cell lines tested as well as in the majority of the human tumor samples [primary (92%) and metastatic (86%)]. Intriguingly, in vitro hMena(+11a) isoform was specifically associated with an epithelial phenotype, EGFR dependency, and sensitivity to erlotinib. In epithelial BxPC3 cells, epidermal growth factor up-regulated hMena/hMena(+11a) and erlotinib down-regulated expression. hMena knockdown reduced cell proliferation and mitogen-activated protein kinase and AKT activation in BxPC3 cells, and promoted the growth inhibitory effects of erlotinib. Collectively, our data indicate that the hMena(+11a) isoform is associated with an epithelial phenotype and identifies EGFR-dependent cell lines that are sensitive to the EGFR inhibitor erlotinib. The availability of anti-hMena(+11a)-specific probes may offer a new tool in pancreatic cancer management if these results can be verified prospectively in cancer patients.

  11. Immunohistochemical Characterization and Sensitivity to Human Adenovirus Serotypes 3, 5, and 11p of New Cell Lines Derived from Human Diffuse Grade II to IV Gliomas

    Directory of Open Access Journals (Sweden)

    Minna Niittykoski

    2017-10-01

    Full Text Available BACKGROUND: Oncolytic adenoviruses show promise in targeting gliomas because they do not replicate in normal brain cells. However, clinical responses occur only in a subset of patients. One explanation could be the heterogenic expression level of virus receptors. Another contributing factor could be variable activity of tumor antiviral defenses in different glioma subtypes. METHODS: We established a collection of primary low-passage cell lines from different glioma subtypes (3 glioblastomas, 3 oligoastrocytomas, and 2 oligodendrogliomas and assessed them for receptor expression and sensitivity to human adenovirus (HAd serotypes 3, 5, and 11p. To gauge the impact of antiviral defenses, we also compared the infectivity of the oncolytic adenoviruses in interferon (IFN-pretreated cells with IFN-sensitive Semliki Forest virus (SFV. RESULTS: Immunostaining revealed generally low expression of HAd5 receptor CAR in both primary tumors and derived cell lines. HAd11p receptor CD46 levels were maintained at moderate levels in both primary tumor samples and derived cell lines. HAd3 receptor DSG-2 was reduced in the cell lines compared to the tumors. Yet, at equal multiplicities of infection, the oncolytic potency of HAd5 in vitro in tumor-derived cells was comparable to HAd11p, whereas HAd3 lysed fewer cells than either of the other two HAd serotypes in 72 hours. IFN blocked replication of SFV, while HAds were rather unaffected. CONCLUSIONS: Adenovirus receptor levels on glioma-derived cell lines did not correlate with infection efficacy and may not be a relevant indicator of clinical oncolytic potency. Adenovirus receptor analysis should be preferentially performed on biopsies obtained perioperatively.

  12. Differential mTOR pathway profiles in bladder cancer cell line subtypes to predict sensitivity to mTOR inhibition.

    Science.gov (United States)

    Hau, Andrew M; Nakasaki, Manando; Nakashima, Kazufumi; Krish, Goutam; Hansel, Donna E

    2017-10-01

    Molecular classification of bladder cancer has been increasingly proposed as a potential tool to predict clinical outcomes and responses to chemotherapy. Here we focused on mechanistic target of rapamycin (mTOR) inhibition as a chemotherapeutic strategy and characterized the expression profile of mTOR signaling targets in representative bladder cancer cell lines from basal, luminal, and either basal/luminal ("non-type") molecular subtypes. Protein and mRNA expression of mTOR signaling components from representative luminal (RT4 and RT112), basal (SCaBER and 5637), and nontype (T24 and J82) bladder cancer cell line subtypes were determined by Western blot and database mining analysis of the Cancer Cell Line Encyclopedia. Cell viability following treatment with either, Torin-2 or KU-0063794, 2 dual mTOR complex 1/2 inhibitors, was determined by MTT assay. Immunoblot analysis of cells treated with Torin-2 or KU-0063794 was performed to determine the effects of mTOR inhibition on expression and phosphorylation status of mTOR signaling components, Akt, 4E-BP1, and ribosomal protein S6. Molecular subtypes of bladder cancer cell lines each exhibited a distinct pattern of expression of mTOR-associated genes and baseline phosphorylation level of Akt and 4E-BP1. Cells with low levels of Akt Ser-473 phosphorylation were more resistant to the cytotoxic effects of mTOR inhibition with Torin-2, but not KU-0063794. Exposure to Torin-2 and KU-0063794 both potently and rapidly inhibited phosphorylation of Akt Ser-473 and Thr-308, and 4E-BP1 T37/46 in cell lines that included basal and nontype subtypes. Differential gene expression and protein activity associated with mTOR signaling is observed among bladder cancer cell lines stratified into basal, luminal, and nontype subtypes. Urothelial carcinomas characterized by high baseline Akt Ser-473 phosphorylation may be best suited for targeted mTOR therapies. Copyright © 2017 Elsevier Inc. All rights reserved.

  13. The sensitivity of lateral line receptors and their role in the behavior of Mexican blind cavefish (Astyanax mexicanus)

    NARCIS (Netherlands)

    Yoshizawa, Masato; Jeffery, William R; van Netten, Sietse M; McHenry, Matthew J

    2014-01-01

    The characid fish species Astyanax mexicanus offers a classic comparative model for the evolution of sensory systems. Populations of this species evolved in caves and became blind while others remained in streams (i.e. surface fish) and retained a functional visual system. The flow-sensitive lateral

  14. An in vitro test to screen skin sensitizers using a stable THP-1-derived IL-8 reporter cell line, THP-G8.

    Science.gov (United States)

    Takahashi, Toshiya; Kimura, Yutaka; Saito, Rumiko; Nakajima, Yoshihiro; Ohmiya, Yoshihiro; Yamasaki, Kenshi; Aiba, Setsuya

    2011-12-01

    Several studies have suggested that interleukin (IL)-8 can serve as a biomarker for discrimination of skin sensitizers from nonsensitizers. We established a stable THP-1-derived IL-8 reporter cell line, THP-G8, which harbors SLO and SLR luciferase genes under the control of IL-8 and glyceraldehyde 3-phosphate dehydrogenase promoters, respectively. After 6 h treatment with chemicals, normalized SLO luciferase activity (nSLO-LA) was calculated by dividing SLO-LA by SLR-LA, and the fold induction of nSLO-LA (FInSLO-LA) was calculated by dividing nSLO-LA of chemically treated cells by that of nontreated cells. The nSLO-LA of THP-G8 cells increased in response to lipopolysaccharide (LPS) and several sensitizers. The FInSLO-LA in THP-G8 cells induced by LPS or sensitizers positively correlated with their induction of IL-8 messenger RNA in THP-1 cells. The nSLO-LA value of THP-G8 cells was significantly increased (FInSLO-LA ≥ 1.4) by 13 of the 15 sensitizers as well as by 5 of the 7 nonsensitizers. Interestingly, pretreatment with N-acetylcysteine suppressed the increase in FInSLO-LA induced by all sensitizers (inhibition index (II) ≤ 0.8) but did not suppress that induced by most of the nonsensitizers. We then evaluated the performance of this assay using values of FInSLO-LA ≥ 1.4 and II ≤ 0.8 in at least two of three independent experiments as the criteria of a sensitizer, which resulted in test accuracies of 82% for the 22 chemicals used and of 88% for the chemicals proposed by European Center for the Validation of Alternative Methods. This newly developed assay is a candidate replacement for animal tests of skin sensitization because of its accuracy, convenience, and high throughput performance.

  15. Highly sensitive in-line microfluidic sensor based on microfiber-assisted Mach-Zehnder interferometer for glucose sensing

    Science.gov (United States)

    Xie, Nanjie; Zhang, Hao; Liu, Bo; Wu, Jixuan; Song, Binbin; Han, Tingting

    2017-11-01

    A highly sensitive microfluidic sensor based on a microfiber-assisted Mach-Zehnder interferometer (MAMZI) is proposed and experimentally demonstrated for the detection of low-concentration glucose solution. A segment of microfiber tapered from standard single-mode fiber (SMF) is spliced between two SMFs with pre-designed lateral offset to constitute the miniaturized MAMZI probe. The transmission spectral response to environmental refractive index variation has been experimentally investigated for glucose concentration ranges of 300 mg dL-1 to 3000 mg dL-1 and 0 to 270 mg dL-1 and the glucose concentration detection limit is 3 mg dL-1, and the experimentally observed transmission spectral responses are in accordance with our theoretical simulation results. Owing to its high sensitivity, non-enzymatic operation method, ease of fabrication and compact size, our proposed MAMZI for glucose sensing is anticipated to be employed in biomedical applications.

  16. Sensitization to oxaliplatin in HCT116 and HT29 cell lines by metformin and ribavirin and differences in response to mitochondrial glutaminase inhibition

    Directory of Open Access Journals (Sweden)

    Silvina M Richard

    2015-01-01

    Full Text Available Aim of study: In the present study, we evaluated the effect of ribavirin and metformin on the sensitivity of oxaliplatin and 5-fluorouracil (5-FU on colon cancer. Materials and Methods: Cell viability of two commercially available colon cancer cell lines (HT29 and HCT116 were analyzed by sulforhodamine B (SRB assay. Results: A clinically achievable and nontoxic concentration of ribavirin and metformin showed a significant synergistic effect on oxaliplatin in HT29 and HCT116 cell lines. Ribavirin showed a synergistic effect on oxaliplatin in HT29 (R = 2.93, P < 0.001 and HCT116 (R = 1.71, P < 0.001, while only in HT29 metformin synergized with oxaliplatin by 2.66 (± 0.28, P < 0.01. In addition, both cell lines showed significant differences in response to Compound 968, inhibitor of mitochondrial glutaminase activity. Conclusion: The data suggested that these cell lines not only turn to metabolic different sustainability process after oxaliplatin treatment but that they also have different basal metabolic requirements of glutamine in vitro which can be exploits in the future for colorectal cancer (CRC treatment and further studies are required.

  17. Factors affecting the sensitivity of human-derived esophageal carcinoma cell lines to 5-fluorouracil and cisplatin

    Science.gov (United States)

    MINEGAKI, TETSUYA; TAKARA, KOHJI; HAMAGUCHI, RYOHEI; TSUJIMOTO, MASAYUKI; NISHIGUCHI, KOHSHI

    2013-01-01

    Effective chemotherapy against esophageal carcinoma is considered achievable with a combination of 5-fluorouracil (5-FU) and cisplatin (CDDP). However, chemo-therapy remains ineffective in certain patients. The aim of this study was to clarify the factors which affect sensitivity to 5-FU and CDDP. The effects of factors known to influence sensitivity to 5-FU and CDDP, namely transporters, DNA repair enzymes and metabolic enzymes, were examined. mRNA levels of four transporters, SLC22A2, SLC23A2, ABCB1 and ABCC2, two DNA repair-related enzymes, Rad51 and MSH2, and one metabolic enzyme, dihydropyrimidine dehydrogenase (DPYD), showed a strong correlation (|r|>0.7) with IC50 values for 5-FU. In addition, the mRNA levels of ABCC2, MSH2 and DPYD showed a strong correlation (|r|>0.7) with the IC50 values for CDDP. Gimeracil, a DPYD inhibitor, enhanced the sensitivity of some cells to 5-FU but decreased the sensitivity of all the cells to CDDP. The inhibitory effects of ABCC2 with MK571 did not correspond to those observed in the correlation analysis. In conclusion, mRNA levels of SLC22A2, SLC23A2, ABCB1, ABCC2, Rad51, MSH2 and DPYD were confirmed to be strongly correlated with IC50 values for 5-FU, and mRNA levels of ABCC2, MSH2 and DPYD were confirmed to be strongly correlated with IC50 values for CDDP. In addition, the inhibition of DPYD appeared to affect the cytotoxicity of CDDP. PMID:23420099

  18. MRI compatibility of position-sensitive photomultiplier depth-of-interaction PET detectors modules for in-line multimodality preclinical studies

    Energy Technology Data Exchange (ETDEWEB)

    Vaquero, J.J., E-mail: juanjose.vaquero@uc3m.es [Universidad Carlos III de Madrid, Departamento de Bioingeniería e Ingeniería Aeroespacial, Avda. de la Universidad 30 Leganés, 28911 Madrid (Spain); Instituto de Investigación Sanitaria Gregorio Marañón, Madrid (Spain); Sánchez, J.J. [Instituto de Investigación Sanitaria Gregorio Marañón, Madrid (Spain); Udías, J.M.; Cal-González, J. [Grupo de Física Nuclear, Departamento de Física Atómica, Molecular y Nuclear, Universidad Complutense de Madrid, CEI Moncloa, Madrid (Spain); Desco, M. [Universidad Carlos III de Madrid, Departamento de Bioingeniería e Ingeniería Aeroespacial, Avda. de la Universidad 30 Leganés, 28911 Madrid (Spain); Instituto de Investigación Sanitaria Gregorio Marañón, Madrid (Spain)

    2013-02-21

    This work addresses the feasibility of a small-animal, in-line PET/MR system based on Position-Sensitive Photo Multiplier Tubes (PS-PMTs). To this end, we measured the effects of static magnetic fields on the PS-PMTs performance in order to explore the minimal tandem separation between the PET and MR subsystems to preserve their respective performances. We concluded that it is possible to achieve minimal degradation of the PET scanner performance (after a system recalibration) if the magnetic field strength influencing the PET detectors is less than 1 mT and if it is oriented perpendicularly to the longitudinal axis of the tube. Therefore, we predict that it will be possible to maintain the PET image quality if it is placed outside the 1 mT line.

  19. Evaluation of the Trajectory Sensitivity Analysis of the DFIG Control Parameters in Response to Changes in Wind Speed and the Line Impedance Connection to the Grid DFIG

    Directory of Open Access Journals (Sweden)

    Mehdi Fooladgar

    2015-01-01

    Full Text Available Economic and environmental conditions often make large stations and transmission lines, restrictions are placed. Small and medium-sized production units connected to existing systems as a strategy is in progress. These units are usually near the center of the load placed and distributed generators (DG famous are the DG are allowed types vary, such as induction generators rack squirrel-connected wind turbines, generators fed induction double mounted wind turbines, fuel cells connected to the system by power electronic converters or synchronous generator connected to the turbine combustion [10]. This way sensitivity analysis in systems of distributed generation (DG is assessed. It is shown that the method can detect the effect of control parameters listed wind turbine connected to a double-fed induction generator (DFIG Badoou the impedance of the changing the speed of on the stability of the transmission line useful system invested. The control parameters of the importance of influencing the behavior of DFIG are divided.

  20. Reconfigurable and ultra-sensitive in-line Mach-Zehnder interferometer based on the fusion of microfiber and microfluid

    Energy Technology Data Exchange (ETDEWEB)

    Gao, Shecheng [Key Laboratory of Optical Information Science and Technology, Ministry of Education, Institute of Modern Optics, Nankai University, Tianjin 300071 (China); Institute of Micro and Nano Optics, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060 (China); Zhang, Weigang, E-mail: zhangwg@nankai.edu.cn, E-mail: haozhang@nankai.edu.cn; Zhang, Hao, E-mail: zhangwg@nankai.edu.cn, E-mail: haozhang@nankai.edu.cn [Key Laboratory of Optical Information Science and Technology, Ministry of Education, Institute of Modern Optics, Nankai University, Tianjin 300071 (China); Zhang, Chonglei [Institute of Micro and Nano Optics, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060 (China)

    2015-02-23

    A reconfigurable Mach-Zenhnder interferometer (MZI) based on a microfluidic cavity (MFC) constructed by embedding a microfiber between two segments of single-mode fibers with pre-designed lateral offset has been proposed and experimentally demonstrated. The MFC serves as an interference arm with an eccentric annular cross section and allows convenient sample (gas or liquids) replacement procedure. The microfiber works as the other interference arm that provides the proposed device with ease of reconstruction and also enhances the force sensitivity. The re-configurability and the ultra-wide tuning sensitivity range are demonstrated by immersing the MZI constructed with a 484 μm-long-MFC and a microfiber 44 μm in diameter in different droplets. Ultrahigh sensitivities of 34.65 nm/°C (∼88 380 nm/RIU) and −493.7 nm/N (∼−590 pm/με) are experimentally achieved using a droplet with a refractive index of ∼1.44.

  1. Systematic assessment of multi-gene predictors of pan-cancer cell line sensitivity to drugs exploiting gene expression data [version 1; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Linh Nguyen

    2016-12-01

    Full Text Available Background: Selected gene mutations are routinely used to guide the selection of cancer drugs for a given patient tumour. Large pharmacogenomic data sets were introduced to discover more of these single-gene markers of drug sensitivity. Very recently, machine learning regression has been used to investigate how well cancer cell line sensitivity to drugs is predicted depending on the type of molecular profile. The latter has revealed that gene expression data is the most predictive profile in the pan-cancer setting. However, no study to date has exploited GDSC data to systematically compare the performance of machine learning models based on multi-gene expression data against that of widely-used single-gene markers based on genomics data. Methods: Here we present this systematic comparison using Random Forest (RF classifiers exploiting the expression levels of 13,321 genes and an average of 501 tested cell lines per drug. To account for time-dependent batch effects in IC50 measurements, we employ independent test sets generated with more recent GDSC data than that used to train the predictors and show that this is a more realistic validation than K-fold cross-validation. Results and Discussion: Across 127 GDSC drugs, our results show that the single-gene markers unveiled by the MANOVA analysis tend to achieve higher precision than these RF-based multi-gene models, at the cost of generally having a poor recall (i.e. correctly detecting only a small part of the cell lines sensitive to the drug. Regarding overall classification performance, about two thirds of the drugs are better predicted by multi-gene RF classifiers. Among the drugs with the most predictive of these models, we found pyrimethamine, sunitinib and 17-AAG. Conclusions: We now know that this type of models can predict in vitro tumour response to these drugs. These models can thus be further investigated on in vivo tumour models.

  2. Fibulin-3 negatively regulates ALDH1 via c-MET suppression and increases γ-radiation-induced sensitivity in some pancreatic cancer cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Kim, In-Gyu, E-mail: igkim@kaeri.re.kr [Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, 989-111 Daedeok-daero, Yuseong-gu, Daejeon 305-353 (Korea, Republic of); Department of Radiation Biotechnology and Applied Radioisotope, Korea University of Science and Technology (UST), 989-111 Daedeok-daero, Yusong-gu, Daejeon 305-353 (Korea, Republic of); Lee, Jae-Ha [Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, 989-111 Daedeok-daero, Yuseong-gu, Daejeon 305-353 (Korea, Republic of); Department of Radiation Biotechnology and Applied Radioisotope, Korea University of Science and Technology (UST), 989-111 Daedeok-daero, Yusong-gu, Daejeon 305-353 (Korea, Republic of); Kim, Seo-Yoen; Kim, Jeong-Yul [Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, 989-111 Daedeok-daero, Yuseong-gu, Daejeon 305-353 (Korea, Republic of); Cho, Eun-Wie [Epigenomics Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806 (Korea, Republic of)

    2014-11-21

    Highlights: • FBLN-3 gene was poorly expressed in some pancreatic cancer lines. • FBLN-3 promoter region was highly methylated in some pancreatic cancer cell lines. • FBLN-3 inhibited c-MET activation and expression and reduced cellular level of ALDH1. • FBLN-3/c-Met/ALDH1 axis modulates stemness and EMT in pancreatic cancer cells. - Abstract: Fibulin-3 (FBLN-3) has been postulated to be either a tumor suppressor or promoter depending on the cell type, and hypermethylation of the FBLN-3 promoter is often associated with human disease, especially cancer. We report that the promoter region of the FBLN-3 was significantly methylated (>95%) in some pancreatic cancer cell lines and thus FBLN-3 was poorly expressed in pancreatic cancer cell lines such as AsPC-1 and MiaPaCa-2. FBLN-3 overexpression significantly down-regulated the cellular level of c-MET and inhibited hepatocyte growth factor-induced c-MET activation, which were closely associated with γ-radiation resistance of cancer cells. Moreover, we also showed that c-MET suppression or inactivation decreased the cellular level of ALDH1 isozymes (ALDH1A1 or ALDH1A3), which serve as cancer stem cell markers, and subsequently induced inhibition of cell growth in pancreatic cancer cells. Therefore, forced overexpression of FBLN-3 sensitized cells to cytotoxic agents such as γ-radiation and strongly inhibited the stemness and epithelial to mesenchymal transition (EMT) property of pancreatic cancer cells. On the other hand, if FBLN3 was suppressed in FBLN-3-expressing BxPC3 cells, the results were opposite. This study provides the first demonstration that the FBLN-3/c-MET/ALDH1 axis in pancreatic cancer cells partially modulates stemness and EMT as well as sensitization of cells to the detrimental effects of γ-radiation.

  3. Genetic variants in cytosolic 5'-nucleotidase II are associated with its expression and cytarabine sensitivity in HapMap cell lines and in patients with acute myeloid leukemia.

    Science.gov (United States)

    Mitra, Amit K; Crews, Kristine R; Pounds, Stanley; Cao, Xueyuan; Feldberg, Tanya; Ghodke, Yogita; Gandhi, Varsha; Plunkett, William; Dolan, M Eileen; Hartford, Christine; Raimondi, Susana; Campana, Dario; Downing, James; Rubnitz, Jeffrey E; Ribeiro, Raul C; Lamba, Jatinder K

    2011-10-01

    Cytosolic 5'-nucleotidase II (NT5C2) is involved in the development of 1-β-d-arabinofuranosylcytosine (ara-C) resistance and has been associated with clinical outcome in patients receiving ara-C-based chemotherapy. NT5C2 inactivates ara-C by dephosphorylating ara-C monophosphate to ara-C. In this study, we sequenced NT5C2 in genomic DNA samples from International HapMap project panels with European [Centre d'Etude du Polymorphisme Humain (CEU); n = 90] or African [Yoruba people in Ibadan, Nigeria (YRI); n = 90] ancestry. We identified 41 genetic variants [one insertion-deletion and 40 single nucleotide polymorphisms (SNPs)], including three nonsynonymous SNPs (Y3A, K47R, and Q136R). Twenty-five SNPs were novel and 16 overlapped with the HapMap data. Subjects with African ancestry had NT5C2 mRNA expression levels that was significantly higher than those with European ancestry (p = 0.005). Furthermore, there was a correlation between NT5C2 mRNA expression and ara-C sensitivity in CEU but not in YRI cell lines. None of the nonsynonymous SNPs demonstrated any effect on NT5C2 activity. The genotypes of several SNPs were significantly associated with NT5C2 mRNA expression and/or ara-C sensitivity in CEU cell lines, but very few were significant in YRI cell lines. Of most interest, SNPs (linkage disequilibrium group CEU.12) in the 5'-untranslated region were associated with NT5C2 expression and ara-C sensitivity in HapMap cell lines and with NT5C2 mRNA expression and ara-C sensitivity in diagnostic leukemic blasts from pediatric patients with acute myeloid leukemia. Functional genomics analysis demonstrated that the promoter SNP rs11191612 was associated with altered luciferase activation in reporter assays and altered DNA-protein binding in gel shift assays. These results suggest that genetic variations in NT5C2 influence its expression and, potentially, cellular responses to nucleoside analogs.

  4. Influence of vitamin D on cisplatin sensitivity in testicular germ cell cancer-derived cell lines and in a NTera2 xenograft model

    DEFF Research Database (Denmark)

    Jørgensen, Anne; Blomberg Jensen, Martin; Nielsen, John Erik

    2013-01-01

    of pluripotency genes and simultaneous upregulation of the cell cycle regulators p21, p27, p53, p73 and FOXO1, while no significant effects were found in TCam-2 and 2102Ep cell lines (derived from seminoma and embryonal carcinoma, respectively). Anti-tumor effects of cholecalciferol, 1,25(OH)(2)D(3....... Future studies are needed to investigate potential beneficial effects of vitamin D with lower cisplatin doses, and to determine whether 1,25(OH)(2)D(3) may increase cisplatin sensitivity in chemotherapy-resistant TGCTs. This article is part of a Special Issue entitled 'Vitamin D Workshop'....

  5. Stathmin decreases cholangiocarcinoma cell line sensitivity to staurosporine-triggered apoptosis via the induction of ERK and Akt signaling

    Science.gov (United States)

    Bo, Xiaobo; Wang, Yaojie; Wang, Jiwen; Shen, Sheng; Liu, Han; Suo, Tao; Pan, Hongtao; Ai, Zhilong; Liu, Houbao

    2017-01-01

    Cholangiocarcinoma is a rare, but highly fatal malignancy. However, the intrinsic mechanism involved in its tumorigenesis remains obscure. An urgent need remains for a promising target for cholangiocarcinoma biological therapies. Based on comparative proteomical technologies, we found 253 and 231 different spots in gallbladder tumor cell lines and cholangiocarcinoma cell lines, respectively, relative to non-malignant cells. Using Mass Spectrometry (MS) and database searching, we chose seven differentially expressed proteins. High Stathmin expression was found in both cholangiocarcinoma and gallbladder carcinoma cells. Stathmin expression was validated using immunohistochemistry and western blot in cholangiocarcinoma tissue samples and peritumoral tissue. It was further revealed that high Stathmin expression was associated with the repression of staurosporine-induced apoptosis in the cholangiocarcinoma cell. Moreover, we found that Stathmin promoted cancer cell proliferation and inhibited its apoptosis through protein kinase B (Akt) and extracellular signal-regulated kinase (ERK) signaling. Integrin, β1 appears to serve as a partner of Stathmin induction of ERK and Akt signaling by inhibiting apoptosis in the cholangiocarcinoma cell. Understanding the regulation of anti-apoptosis effect by Stathmin might provide new insight into how to overcome therapeutic resistance in cholangiocarcinoma. PMID:28178656

  6. Evaluation of the sensitizing potential of antibiotics in vitro using the human cell lines THP-1 and MUTZ-LC and primary monocyte-derived dendritic cells.

    Science.gov (United States)

    Sebastian, Katrin; Ott, Hagen; Zwadlo-Klarwasser, Gabriele; Skazik-Voogt, Claudia; Marquardt, Yvonne; Czaja, Katharina; Merk, Hans F; Baron, Jens Malte

    2012-08-01

    Since the 7th amendment to the EU cosmetics directive foresees a complete ban on animal testing, alternative in vitro methods have been established to evaluate the sensitizing potential of small molecular weight compounds. To find out whether these novel in vitro assays are also capable to predict the sensitizing potential of small molecular weight drugs, model compounds such as beta-lactams and sulfonamides - which are the most frequent cause of adverse drug reactions - were co-incubated with THP-1, MUTZ-LC, or primary monocyte-derived dendritic cells for 48 h and subsequent expression of selected marker genes (IL-8, IL-1β, CES1, NQO1, GCLM, PIR and TRIM16) was studied by real time PCR. Benzylpenicillin and phenoxymethylpenicillin were recognized as sensitizing compounds because they are capable to induce the mRNA expression of these genes in moDCs and, except for IL-8, in THP-1 cells but not in MUTZ-LC. Ampicillin stimulated the expression of some marker genes in moDCs and THP-1 cells. SMX did not affect the expression of these genes in THP-1, however, in moDCs, at least PIR was enhanced and there was an increase of the release of IL-8. These data reveal that novel in vitro DC based assays might play a role in the evaluation of the allergenic potential of novel drug compounds, but these systems seem to lack the ability to detect the sensitizing potential of prohaptens that require metabolic activation prior to sensitization and moDCs seem to be superior with regard to the sensitivity compared with THP-1 and MUTZ-3 cell lines. Copyright © 2012 Elsevier Inc. All rights reserved.

  7. Cell Line Derived 5-FU and Irinotecan Drug-Sensitivity Profiles Evaluated in Adjuvant Colon Cancer Trial Data

    DEFF Research Database (Denmark)

    Buhl, Ida Kappel; Gerster, Sarah; Delorenzi, Mauro

    2016-01-01

    patients enrolled in the PETACC-3 prospective randomized clinical trial. A 5-FU profile developed similarly was assessed by comparing the PETACC-3 cohort with a cohort of 359 stage II colon cancer patients who underwent surgery but received no adjuvant therapy. RESULTS: There was no statistically...... patients who benefitted from the addition of irinotecan to 5-FU, we used gene expression profiles based on cell lines and clinical tumor material. These profiles were applied to expression data obtained from pretreatment formalin fixed paraffin embedded (FFPE) tumor tissue from 636 stage III colon cancer...... to identify colon cancer patients who may benefit from 5-FU, however, any biomarker predicting benefit for adjuvant 5-FU must be rigorously evaluated in independent cohorts. Given differences between the two study cohorts, the present results should be further validated....

  8. Sensitivity of glacier mass balance and equilibrium line altitude to climatic change on King George Island, Antarctic Peninsula.

    Science.gov (United States)

    Falk, Ulrike; Lopez, Damian; Silva-Busso, Adrian

    2017-04-01

    The South Shetland Islands are located at the northern tip of the Antarctic Peninsula which is among the fastest warming regions on Earth. Surface air temperature increases (ca. 3 K in 50 years) are concurrent with retreating glacier fronts, an increase in melt areas, ice surface lowering and rapid break-up and disintegration of ice shelves. Observed surface air temperature lapse rates show a high variability during winter months (standard deviations up to ±1.0 K/100 m), and a distinct spatial heterogeneity reflecting the impact of synoptic weather patterns especially during winter glacial mass accumulation periods. The increased mesocyclonic activity during the winter time in the study area results in intensified advection of warm, moist air with high temperatures and rain, and leads to melt conditions on the ice cap, fixating surface air temperatures to the melting point. The impact on winter accumulation results in even more negative mass balance estimates. Six years of glaciological measurements on mass balance stake transects are used with a glacier melt model to assess changes in melt water input to the coastal waters, glacier surface mass balance and the equilibrium line altitude. The average equilibrium line altitude (ELA) calculated from own glaciological observations for KGI over the time period 2010 - 2015 amounts to ELA=330±100 m. Published studies suggest rather stable condition slightly negative glacier mass balance until the mid 80's with an ELA of approx. 150 m. The calculated accumulation area ratio suggests rather dramatic changes in extension of the inland ice cap for the South Shetland Islands until an equilibrium with concurrent climate conditions is reached.

  9. Reversal effect of arsenic sensitivity in human leukemia cell line K562 and K562/ADM using realgar transforming solution.

    Science.gov (United States)

    Wang, Xin; Zhang, Xu; Xu, Zhiliang; Wang, Zhizeng; Yue, Xiaoxuan; Li, Hongyu

    2013-01-01

    The success of arsenic trioxide (ATO) in treatment of acute promyelocytic leukemia (APL) attracts a great deal of attention to researchers to explore its activity of anti-leukemia. However, ATO has unavailable effect on chronic myeloid leukemia (CML), especially multidrug resistant (MDR)-CML, unless using high concentration. Realgar (As(4)S(4)) has been employed in Chinese traditional medicine for 1500 years. Research evidences confirmed realgar has similar effect on treating with APL as ATO, but the problem of large dose and long period in the CML/MDR-CML treatment still exist. By using a microbial leaching process with Acidithiobacillus ferrooxidans, we obtained realgar transforming solution (RTS) which showed significantly higher extent in inhibiting CML cell line K562 and MDR-CML cell line K562/ADM, and then trigger apoptosis. Both K562 and K562/ADM showed arsenic-dose-dependent effect on RTS. Interestingly, the overexpression of MDR1 mRNA and P-glucoprotein (P-gp) in K562/ADM cells were down-regulated by RTS, where there are no obvious effects on ATO and realgar and arsenic can be subsequently accumulated in K562/ADM cells efficiently. The intracellular accumulation of arsenic in K562/ADM cells treated with RTS for 4 h was 2-fold and 16-folds higher than those treated with realgar or ATO. Meanwhile, Western blot analysis of AQP9, the main transporter of arsenic, was increased by RTS treatment particularly in K562/ADM. Thus, these results suggested that the effect from a certain arsenical or a variety of arsenicals in RTS might be a promising candidate both for treating CML/MDR-CML alone and as combinations with currently used anti-CML/MDR-CML drug, although arsenical forms in RTS are undefined.

  10. Evaluation of the sensitizing potential of antibiotics in vitro using the human cell lines THP-1 and MUTZ-LC and primary monocyte‐derived dendritic cells

    Energy Technology Data Exchange (ETDEWEB)

    Sebastian, Katrin, E-mail: ksebastian@ukaachen.de [Department of Dermatology and Allergology, RWTH Aachen University Hospital, D-52074 Aachen (Germany); Ott, Hagen [Department of Dermatology and Allergology, RWTH Aachen University Hospital, D-52074 Aachen (Germany); Zwadlo-Klarwasser, Gabriele [IZKF (BIOMAT), RWTH Aachen University Hospital, D-52074 Aachen (Germany); Skazik-Voogt, Claudia; Marquardt, Yvonne; Czaja, Katharina; Merk, Hans F.; Baron, Jens Malte [Department of Dermatology and Allergology, RWTH Aachen University Hospital, D-52074 Aachen (Germany)

    2012-08-01

    Since the 7th amendment to the EU cosmetics directive foresees a complete ban on animal testing, alternative in vitro methods have been established to evaluate the sensitizing potential of small molecular weight compounds. To find out whether these novel in vitro assays are also capable to predict the sensitizing potential of small molecular weight drugs, model compounds such as beta-lactams and sulfonamides – which are the most frequent cause of adverse drug reactions – were co-incubated with THP-1, MUTZ-LC, or primary monocyte‐derived dendritic cells for 48 h and subsequent expression of selected marker genes (IL-8, IL-1β, CES1, NQO1, GCLM, PIR and TRIM16) was studied by real time PCR. Benzylpenicillin and phenoxymethylpenicillin were recognized as sensitizing compounds because they are capable to induce the mRNA expression of these genes in moDCs and, except for IL-8, in THP-1 cells but not in MUTZ-LC. Ampicillin stimulated the expression of some marker genes in moDCs and THP-1 cells. SMX did not affect the expression of these genes in THP-1, however, in moDCs, at least PIR was enhanced and there was an increase of the release of IL-8. These data reveal that novel in vitro DC based assays might play a role in the evaluation of the allergenic potential of novel drug compounds, but these systems seem to lack the ability to detect the sensitizing potential of prohaptens that require metabolic activation prior to sensitization and moDCs seem to be superior with regard to the sensitivity compared with THP-1 and MUTZ-3 cell lines. -- Highlights: ► We tested the sensitizing potential of small molecular weight drugs in vitro. ► In vitro assays were performed with moDCs and THP-1 cells. ► Beta-lactam antibiotics can be recognized as sensitizing compounds. ► They affect the expression of metabolic enzymes, cytokines and transcription factors. ► Sulfamethoxazole has no measurable effect on THP-1 cells and moDCs.

  11. Systematic assessment of multi-gene predictors of pan-cancer cell line sensitivity to drugs exploiting gene expression data [version 2; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Linh Nguyen

    2017-03-01

    Full Text Available Background: Selected gene mutations are routinely used to guide the selection of cancer drugs for a given patient tumour. Large pharmacogenomic data sets, such as those by Genomics of Drug Sensitivity in Cancer (GDSC consortium, were introduced to discover more of these single-gene markers of drug sensitivity. Very recently, machine learning regression has been used to investigate how well cancer cell line sensitivity to drugs is predicted depending on the type of molecular profile. The latter has revealed that gene expression data is the most predictive profile in the pan-cancer setting. However, no study to date has exploited GDSC data to systematically compare the performance of machine learning models based on multi-gene expression data against that of widely-used single-gene markers based on genomics data. Methods: Here we present this systematic comparison using Random Forest (RF classifiers exploiting the expression levels of 13,321 genes and an average of 501 tested cell lines per drug. To account for time-dependent batch effects in IC50 measurements, we employ independent test sets generated with more recent GDSC data than that used to train the predictors and show that this is a more realistic validation than standard k-fold cross-validation. Results and Discussion: Across 127 GDSC drugs, our results show that the single-gene markers unveiled by the MANOVA analysis tend to achieve higher precision than these RF-based multi-gene models, at the cost of generally having a poor recall (i.e. correctly detecting only a small part of the cell lines sensitive to the drug. Regarding overall classification performance, about two thirds of the drugs are better predicted by the multi-gene RF classifiers. Among the drugs with the most predictive of these models, we found pyrimethamine, sunitinib and 17-AAG. Conclusions: Thanks to this unbiased validation, we now know that this type of models can predict in vitro tumour response to some of these

  12. A gallotannin-rich fraction from Caesalpinia spinosa (Molina) Kuntze displays cytotoxic activity and raises sensitivity to doxorubicin in a leukemia cell line.

    Science.gov (United States)

    Castañeda, Diana M; Pombo, Luis Miguel; Urueña, Claudia Patricia; Hernandez, John Fredy; Fiorentino, Susana

    2012-04-10

    Enhancement of tumor cell sensitivity may help facilitate a reduction in drug dosage using conventional chemotherapies. Consequently, it is worthwhile to search for adjuvants with the potential of increasing chemotherapeutic drug effectiveness and improving patient quality of life. Natural products are a very good source of such adjuvants. The biological activity of a fraction enriched in hydrolysable polyphenols (P2Et) obtained from Caesalpinia spinosa was evaluated using the hematopoietic cell line K562. This fraction was tested alone or in combination with the conventional chemotherapeutic drugs doxorubicin, vincristine, etoposide, camptothecin and taxol. The parameters evaluated were mitochondrial depolarization, caspase 3 activation, chromatin condensation and clonogenic activity. We found that the P2Et fraction induced mitochondrial depolarization, activated caspase 3, induced chromatin condensation and decreased the clonogenic capacity of the K562 cell line. When the P2Et fraction was used in combination with chemotherapeutic drugs at sub-lethal concentrations, a fourfold reduction in doxorubicin inhibitory concentration 50 (IC50) was seen in the K562 cell line. This finding suggested that P2Et fraction activity is specific for the molecular target of doxorubicin. Our results suggest that a natural fraction extracted from Caesalpinia spinosa in combination with conventional chemotherapy in combination with natural products on leukemia cells may increase therapeutic effectiveness in relation to leukemia.

  13. A combination of positive dielectrophoresis driven on-line enrichment and aptamer-fluorescent silica nanoparticle label for rapid and sensitive detection of Staphylococcus aureus.

    Science.gov (United States)

    Shangguan, Jingfang; Li, Yuhong; He, Dinggeng; He, Xiaoxiao; Wang, Kemin; Zou, Zhen; Shi, Hui

    2015-07-07

    Staphylococcus aureus (S. aureus) is an important human pathogen that causes several diseases ranging from superficial skin infections to life-threatening diseases. Here, a method combining positive dielectrophoresis (pDEP) driven on-line enrichment and aptamer-fluorescent silica nanoparticle label has been developed for the rapid and sensitive detection of S. aureus in microfluidic channels. An aptamer, having high affinity to S. aureus, is used as the molecular recognition tool and immobilized onto chloropropyl functionalized fluorescent silica nanoparticles through a click chemistry approach to obtain S. aureus aptamer-nanoparticle bioconjugates (Apt(S.aureus)/FNPs). The pDEP driven on-line enrichment technology was used for accumulating the Apt(S.aureus)/FNP labeled S. aureus. After incubating with S. aureus, the mixture of Apt(S.aureus)/FNP labeled S. aureus and Apt(S.aureus)/FNPs was directly introduced into the pDEP-based microfluidic system. By applying an AC voltage in a pDEP frequency region, the Apt(S.aureus)/FNP labelled S. aureus moved to the electrodes and accumulated in the electrode gap, while the free Apt(S.aureus)/FNPs flowed away. The signal that came from the Apt(S.aureus)/FNP labelled S. aureus in the focused detection areas was then detected. Profiting from the specificity of aptamer, signal amplification of FNP label and pDEP on-line enrichment, this assay can detect as low as 93 and 270 cfu mL(-1)S. aureus in deionized water and spiked water samples, respectively, with higher sensitivities than our previously reported Apt(S.aureus)/FNP based flow cytometry. Moreover, without the need for separation and washing steps usually required for FNP label involved bioassays, the total assay time including sample pretreatment was within 2 h.

  14. Mechanism of paclitaxel resistance in a human prostate cancer cell line, PC3-PR, and its sensitization by cabazitaxel.

    Science.gov (United States)

    Sobue, Sayaka; Mizutani, Naoki; Aoyama, Yuka; Kawamoto, Yoshiyuki; Suzuki, Motoshi; Nozawa, Yoshinori; Ichihara, Masatoshi; Murate, Takashi

    2016-10-28

    Paclitaxel (PTX) is a microtubule-targeting drug widely used for the treatment of a variety of cancers. However, drug resistance can emerge after a series of treatments, and this can seriously affect the patient's prognosis. Here, we analyzed the mechanism of PTX resistance using a human prostate cancer cell line, PC3, and its PTX-resistant subline, PC3-PR. Compared with PC3, PC3-PR exhibited some unique phenotypes that might be associated with PTX resistance, including decreased expression of acetylated α-tubulin and the cell cycle regulator p21, and increased expression of βIII tubulin, histone deacetylase 6 (HDAC6), and the anti-apoptotic protein Bcl2. The drug exporters MDR1 and MRP1 were not involved in PTX resistance. Although cabazitaxel (CTX), a novel taxoid, has been reported to overcome PTX resistance, its mechanism of action is unknown. We found that treatment of PC3-PR cells with CTX induced expression of acetylated α-tubulin and p21, but not the related regulators p27, p15, and p16 or the Bcl2 family proteins. The pan-HDAC inhibitors trichostatin A and suberanilohydroxamic acid and the HDAC6-specific inhibitor tubacin inhibited PC3-PR proliferation and increased expression of p21 and acetylated α-tubulin in a manner similar to CTX. Our data shed light on the cellular response to PTX and CTX. Copyright © 2016 Elsevier Inc. All rights reserved.

  15. Bolometer's development for the detection of dark matter; Instrumentation autour de bolometres pour la recherche de matiere sombre WIMPs

    Energy Technology Data Exchange (ETDEWEB)

    Yvon, D

    2000-06-01

    The author reviews his contributions to the use of bolometers (cryogenic detectors) for the detection of wimps (weakly interactive massive particles). Wimps are detected through their elastic scattering on the nuclei of the detector, a heat signal, luminescence or ionization can be simultaneously detected (at least 2 signals are necessary to discard photon interactions). Bolometers operate at low temperatures (< 50 mK) so they allow very low detection threshold and resolution (< keV) with a full energy conversion for recoiling nuclei. In Saclay the technology of bolometers based on simultaneous detection of heat and ionisation has been developed and improvements have been studied (NbSi thin films bolometers). The first results obtained in the framework of the Edelweiss collaboration are presented. Other developments based on infra-red bolometry (Planck surveyor and Archeops projects) are briefly described. In an appendix the operating principle of a bolometer is presented. (A.C.)

  16. Ferrocene-cinchona hybrids with triazolyl-chalcone linkers act as pro-oxidants and sensitize human cancer cell lines to paclitaxel.

    Science.gov (United States)

    Podolski-Renić, Ana; Bősze, Szilvia; Dinić, Jelena; Kocsis, László; Hudecz, Ferenc; Csámpai, Antal; Pešić, Milica

    2017-08-16

    Recently, we demonstrated that ferrocene-containing compounds with a cinchona moiety displayed marked anticancer activity. Here we report on the effects of the most promising isomers encompassing quinine- (compounds 4 and 5) and quinidine-epimers (compounds 6 and 7) - synthesized using improved methods providing controlled diastereoselectivity - in three different human multidrug resistant (MDR) cancer cell lines and their sensitive counterparts (non-small cell lung carcinoma NCI-H460/R/NCI-H460, colorectal carcinoma DLD1-TxR/DLD1 and glioblastoma U87-TxR/U87). We observed that the presence of the MDR phenotype did not diminish the activity of the compounds suggesting that ferrocene quinine- and quinidine-epimers are not substrates for P-glycoprotein, which has been indicated as a major mechanism of MDR in the cell lines used. Considering that metal-based anticancer agents mainly act by increasing ROS production, we investigated the potential of ferrocene-quinidine epimers to generate ROS. We found that 6 and 7 more readily increased ROS production and induced mitochondrial damage in MDR cancer cells. According to cell death analysis, 6 and 7 were more active against MDR cancer cells showing collateral sensitivity. In addition, our data suggest that these compounds could act as inhibitors of autophagy. Importantly, simultaneous treatments of 6 and 7 with paclitaxel (PTX) increased the sensitivity of MDR cancer cells to PTX. In conclusion, the ferrocene-quinidine epimers, besides being selective towards MDR cancer cells, could also possess potential to overcome PTX resistance.

  17. Highly Sensitive Raman Spectroscopy with Low Laser Power for Fast In-Line Reaction and Multiphase Flow Monitoring.

    Science.gov (United States)

    Braun, Frank; Schwolow, Sebastian; Seltenreich, Julia; Kockmann, Norbert; Röder, Thorsten; Gretz, Norbert; Rädle, Matthias

    2016-10-04

    In process analytics, the applicability of Raman spectroscopy is restricted by high excitation intensities or the long integration times required. In this work, a novel Raman system was developed to minimize photon flux losses. It allows specific reduction of spectral resolution to enable the use of Raman spectroscopy for real-time analytics when strongly increased sensitivity is required. The performance potential of the optical setup was demonstrated in two exemplary applications: First, a fast exothermic reaction (Michael addition) was monitored with backscattering fiber optics under strongly attenuated laser power (7 mW). Second, high-speed scanning of a segmented multiphase flow (water/toluene) with submicroliter droplets was achieved by aligning the focus of a coaxial Raman probe with long focal length directly into a perfluoroalkoxy (PFA) capillary. With an acquisition rate of 333 Raman spectra per second, chemical information was obtained separately for both of the rapidly alternating phases. The experiment with reduced laser power demonstrates that the technique described in this paper is applicable in chemical production processes, especially in hazardous environments. Further potential uses can be envisioned in medical or biological applications with limited power input. The realization of high-speed measurements shows new possibilities for analysis of heterogeneous phase systems and of fast reactions or processes.

  18. Behavioural thermoregulation in a temperature-sensitive coral reef fish, the five-lined cardinalfish ( Cheilodipterus quinquelineatus)

    Science.gov (United States)

    Nay, Tiffany J.; Johansen, Jacob L.; Habary, Adam; Steffensen, John F.; Rummer, Jodie L.

    2015-12-01

    As global temperatures increase, fish populations at low latitudes are thought to be at risk as they are adapted to narrow temperature ranges and live at temperatures close to their thermal tolerance limits. Behavioural movements, based on a preference for a specific temperature ( T pref), may provide a strategy to cope with changing conditions. A temperature-sensitive coral reef cardinalfish ( Cheilodipterus quinquelineatus) was exposed to 28 °C (average at collection site) or 32 °C (predicted end-of-century) for 6 weeks. T pref was determined using a shuttlebox system, which allowed fish to behaviourally manipulate their thermal environment. Regardless of treatment temperature, fish preferred 29.5 ± 0.25 °C, approximating summer average temperatures in the wild. However, 32 °C fish moved more frequently to correct their thermal environment than 28 °C fish, and daytime movements were more frequent than night-time movements. Understanding temperature-mediated movements is imperative for predicting how ocean warming will influence coral reef species and distribution patterns.

  19. Differential modulation of nuclear texture, histone acetylation, and MDR1 gene expression in human drug-sensitive and -resistant OV1 cell lines.

    Science.gov (United States)

    Yatouji, Sonia; El-Khoury, Victoria; Trentesaux, Chantal; Trussardi-Regnier, Aurelie; Benabid, Rym; Bontems, Franck; Dufer, Jean

    2007-04-01

    Image cytometric study of pathological specimens or cell lines has suggested that epigenetic mechanisms are likely to play a major role in determining chromatin patterns evaluable through nuclear texture analysis. We previously reported that nuclear textural changes observed in the OV1-VCR etoposide-resistant ovarian carcinoma cell line were associated with an increased acetylated histone H4 level. In this study we analyzed the effects of treatments with the HDAC inhibitor trichostatin A (TSA) or with nickel subsulfide on histone H4 acetylation, nuclear texture, and MDR1 gene expression in drug-sensitive IGROV1 and drug-resistant OV1-VCR cell lines. In IGROV1 cells, TSA induced an increase in acetylated H4 level associated with a chromatin textural decondensation and an increase in MDR1 gene expression. In OV1-VCR cells, a similar increase in H4 acetylation was observed, but nuclear texture or MDR1 gene expression remained unchanged. ChIP analysis revealed that MDR1 gene expression remained stable in TSA-treated OV1-VCR cells despite a localized increase in H4 acetylation at the promoter level. Analysis of the methylation status of MDR1 promoter showed an increase in DNA methylation at 3 specific sites in OV1-VCR cells, that could participate to TSA low responsiveness in these cells. Treatment with nickel subsulfide induced a decrease in H4 acetylation without any effect on nuclear texture characteristics in both cell lines. In OV1-VCR cells, nickel subsulfide induced a significant down-regulation of the MDR1 gene expression. These results indicate that modulation of histone H4 acetylation level can be associated with up- or down-regulation of the MDR1 gene in OV1 cells. However, this modulation does not always result in chromatin pattern alterations and these data emphasize the complexity of chromatin texture regulation in tumor cells.

  20. Acquired TGF beta 1 sensitivity and TGF beta 1 expression in cell lines established from a single small cell lung cancer patient during clinical progression.

    Science.gov (United States)

    Nørgaard, P; Damstrup, L; Rygaard, K; Spang-Thomsen, M; Poulsen, H S

    1996-02-01

    Three small cell lung cancer cell lines established from a single patient during longitudinal follow-up were examined for in vitro expression of TGF beta and TGF beta receptors, i.e. the components of an autocrine loop. GLC 14 was established prior to treatment, GLC 16 on relapse after chemotherapy and GLC 19 on recurrence after radiotherapy. TGF beta was detected by ELISA and TGF beta receptors by chemical crosslinking to radiolabelled TGF beta 1. Furthermore, TGF beta and TGF beta receptor mRNAs were detected by northern blot analysis. Expression of type II TGF beta receptor mRNA and protein was found in GLC 16 and GLC 19. These cell lines were also growth inhibited by exogenously administrated TGF beta 1. TGF beta 1 mRNA and protein in its latent form was only expressed in the radiotherapy-resistant cell line, GLC 19. The results indicate that disease progression in this patient was paralleled by a gain in sensitivity to the growth inhibition by TGF beta 1 due to type II TGF beta receptor, and a gain of latent TGF beta 1 protein. Lack of type II receptor expression in GLC 14, which was also resistant to growth inhibition by exogenous TGF beta 1, was not due to gross structural changes in the type II receptor gene, as examined by Southern blotting. Also, the type I receptor could not be detected by ligand binding assay in this cell line, despite expression of mRNA for this receptor. This agrees with previous findings that type I receptor cannot bind TGF beta 1 without co-expression of the type II receptor.

  1. Use of the human monocytic leukemia THP-1 cell line and co-incubation with microsomes to identify and differentiate hapten and prohapten sensitizers.

    Science.gov (United States)

    Chipinda, Itai; Ruwona, Tinashe B; Templeton, Steven P; Siegel, Paul D

    2011-02-27

    Consumer and medical products can contain leachable chemical allergens which can cause skin sensitization. Recent efforts have been directed at the development of non-animal based tests such as in vitro cell activation assays for the identification of skin sensitizers. Prohapten identification by in vitro assays is still problematic due to the lack of prohapten bioactivation. The present study evaluated the effect of hapten and prohapten exposure on cell surface markers expression (CD86, CD54 and CD40) in the human monocytic leukemia, THP-1, cell line. Upregulation of activation and costimulatory markers are key events in the allergic sensitization process and have been reported to serve as indicators of skin sensitization. Cells were exposed to the prohaptens benzo(a)pyrene (BaP), 7,12-dimethylbenz(a)anthracene (DMBA), carvone oxime (COx), cinnamic alcohol (CA) and isoeugenol (IEG) at concentrations ranging from 1 to 10 μM for 24 and 48 h. The direct-binding haptens dinitrochlorobenzene (DNCB), benzoquinone (BQ), hydroxylethyl acrylate (HEA) and benzylbromide (BB) were used as positive controls. Cells were also exposed to the irritants sodium dodecyl sulfate (SDS) and sulfanilamide (SFA). Bioactivation of prohaptens was achieved by adding aroclor-induced rat liver microsomes (S9) to the cell cultures. Consistent upregulation of surface expressions of CD86, CD54 (ICAM-1) and CD40 was observed in THP-1 cells treated with direct-acting haptens (±S9) or prohapten (+S9). Upregulation of these markers was not observed after exposure to skin irritants or prohaptens in the absence of exogenously added S9. In conclusion, modification of in vitro cell culture assays to include co-incubation with microsomes enhances identification of prohaptens and allows them to be clearly distinguished from direct-binding haptens. Published by Elsevier Ireland Ltd.

  2. Gene Expression Analysis of the IPEC-J2 Cell Line: A Simple Model for the Inflammation-Sensitive Preterm Intestine

    DEFF Research Database (Denmark)

    Støy, Ann Cathrine Findal; Heegaard, Peter M. H.; Sangild, Per T.

    2013-01-01

    The IPEC-J2 cell line was studied as a simple model for investigating responses of the newborn intestinal epithelium to diets. Especially, the small intestine of immature newborns is sensitive to diet-induced inflammation. We investigated gene expression of epithelial- and immune response...... preterm pigs, both used as models for the newborn intestine, underline that caution must be exercised prior to analysis and interpretation of diet-induced effects on gene expression......-related genes in IPEC-J2 cells stimulated for 2 h with milk formula (CELL-FORM), colostrum (CELL-COLOS), or growth medium (CELL-CONTR) and in distal small intestinal tissue samples from preterm pigs fed milk formula (PIG-FORM) or colostrum (PIG-COLOS). High throughput quantitative PCR analysis of 48 genes...

  3. The impact of insulin on chemotherapeutic sensitivity to 5-fluorouracil in gastric cancer cell lines SGC7901, MKN45 and MKN28.

    Science.gov (United States)

    Wei, Zhao; Liang, Li; Junsong, Liu; Rui, Chen; Shuai, Chang; Guanglin, Qiu; Shicai, He; Zexing, Wang; Jin, Wang; Xiangming, Che; Shufeng, Wang

    2015-06-18

    The role of insulin in the pathogenesis of cancer has been increasingly emphasized because of the high incidence of obesity and metabolic syndrome and their correlated complication including cancer. This study aimed to explore the impact of insulin on chemoresistance to 5-fluorouracil in gastric cancer and the possible mechanisms. Tissue samples of gastric cancer and adjacent normal gastric mucosa from patients with or without obesity were performed immunohistochemical staining for P-glycoprotein. The follow-up was done after the surgical treatment. The effect of insulin on chemotherapeutic sensitivity of the three gastric cancer cell lines to 5-fluorouracil was evaluated by pre-incubation with insulin before administration of 5-fluorouracil. The expression of P-glycoprotein was determined by Western blotting. P-glycoprotein were overexpressed in tissues from patients who suffered gastric cancer and were higher in those simultaneously suffered gastric cancer and obesity. Addition of 1 μM insulin remarkably promoted the proliferation of SGC7901, MKN45 and MKN28 cells and decreased the cytotoxicity of 5-fluorouracil. In addition, the expression of P-glycoprotein was upregulated in SGC7901, MKN45 and MKN28 cells. Insulin improved the proliferation of gastric cancer cell lines and contributed to chemoresistance of gastric cancer cells to 5-fluorouracil which is likely to involve upregulation of P-glycoprotein.

  4. Development of a clone from established Bovine Kidney (BK cell line and evaluation of its sensitivity to Parainfluenza type 3 and Herpes Simplex type 1 viruses.

    Directory of Open Access Journals (Sweden)

    Yashar Mohammadzadeh sedigh

    2009-09-01

    Full Text Available Background: Application of continuous cell lines has got a special place in the virological researches. These cells are immortal and their chromosomes are aneuploid. Therefore, they can be passage without any limitation. The aim of this research was to choose the best way of producing clone of cells. Methods: in this study, Bovine Kidney (BK cell line was used to be cloned through limiting dilution method in which Vero cells were used as feeder layer. Vero cells were first cultured in DMEM supplimented with 7% heat inactivated calf serum and after a monolayer were formed, their growth was arrested by Mitomycin C. The cloned cells after incubation were separated and cultured in a new flask. After several experiments different clones were obtained and cultured for further studies. Results: Karyotype of clone cells were determined and compared with original cells. It was shown that cloned cells were more homogenous in early passages and their karyotypes showed less variability than original ones. Cloned and original cells were inoculated with HSV-1 and Parainfluenza virus 3 in order to evaluate its biological abilities. Tissue culture of infectious dose 50 (TCID50 of each virus was calculated and it was shown that there was no significant different between the HSV-1 titers before and after cloning whereas the titer of the Parainfluenza virus 3 was significantly higher in the original cells. Conclusions: Cloned cells of BK showed more stable karyotype and were less sensitive to parainfluenza type-3 virus infection than original BK cells.

  5. Synthesis of 17β-N-arylcarbamoylandrost-4-en-3-one derivatives and their anti-proliferative effect on human androgen-sensitive LNCaP cell line.

    Science.gov (United States)

    Cortés-Benítez, Francisco; Cabeza, Marisa; Ramírez-Apan, María Teresa; Alvarez-Manrique, Berenice; Bratoeff, Eugene

    2016-10-04

    In this study, we report the synthesis and anti-proliferative effect of a set of eight androst-4-ene-3-one derivatives with different arylcarbamoyl groups at C-17. The novel compounds were prepared from commercially available 3β-hydroxy-5-pregnen-20-one and evaluated against the androgen-sensitive human prostate adenocarcinoma LNCaP cell line. The cancerous cells were exposed to 50 μM of each compound and the proliferating agent testosterone (T) or dihydrotestosterone (DHT). The most potent compounds from this assay were further tested against the androgen-insensitive PC3 cell line. We also demonstrate the activity of these compounds on rat peripheral blood mononuclear cells for comparison. Both 17β-N-[3,5-bis(trifluoromethyl)phenylcarbamoyl]androst-4-ene-3-one (6f) and 17β-N-(1,3-thiazol-2-ylcarbamoyl)androst-4-ene-3-one (6g) exhibited a higher growth inhibitory effect than commercially available drugs finasteride, flutamide and ketoconazole on LNCaP cells in the presence and absence of androgens. In addition, 6f and 6g demonstrated high potency on PC3 cells suggesting an androgen-independent anti-proliferative effect. Moreover, the novel compounds showed a small effect on rat mononuclear cells, an indication of low toxicity. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  6. Establishment and functional characterization of a tracheal epithelial cell line RTEC11 from transgenic rats harboring temperature-sensitive simian virus 40 large T-antigen.

    Science.gov (United States)

    Tabuchi, Yoshiaki; Doi, Takeshi; Takasaki, Ichiro; Takahashi, Ri-ichi; Ueda, Masatsugu; Suzuki, Yoshihisa; Obinata, Masuo

    2008-11-01

    A tracheal epithelial cell line RTEC11 was established from transgenic rats harboring temperature-sensitive simian virus 40 large T-antigen. The cells grew continuously at a permissive temperature of 33 degrees C but not at a non-permissive temperature of 39 degrees C. Morphological and functional investigations demonstrated that the cells were polarized epithelial cells maintaining a regulated permeability barrier function. Interestingly, the expression levels of Muc1 (mucin 1) and Scgb1a1 (uteroglobin), non-ciliated secretory cell markers, and Tubb4 (tubulin beta 4), a ciliated cell marker, were significantly increased under the cell growth-restricted condition. Global gene expression and computational network analyses demonstrated a significant genetic network associated with cellular development and differentiation in cells cultured at the non-permissive temperature. The tracheal epithelial cell line RTEC11 with unique characteristics should be useful as an in vitro model for studies of the physiological functions and gene expression of tracheal epithelial cells.

  7. First 5 tower WIMP-search results from the Cryogenic Dark Matter Search with improved understanding of neutron backgrounds and benchmarking

    Energy Technology Data Exchange (ETDEWEB)

    Hennings-Yeomans, Raul [Case Western Reserve Univ., Cleveland, OH (United States)

    2009-02-01

    Non-baryonic dark matter makes one quarter of the energy density of the Universe and is concentrated in the halos of galaxies, including the Milky Way. The Weakly Interacting Massive Particle (WIMP) is a dark matter candidate with a scattering cross section with an atomic nucleus of the order of the weak interaction and a mass comparable to that of an atomic nucleus. The Cryogenic Dark Matter Search (CDMS-II) experiment, using Ge and Si cryogenic particle detectors at the Soudan Underground Laboratory, aims to directly detect nuclear recoils from WIMP interactions. This thesis presents the first 5 tower WIMP-search results from CDMS-II, an estimate of the cosmogenic neutron backgrounds expected at the Soudan Underground Laboratory, and a proposal for a new measurement of high-energy neutrons underground to benchmark the Monte Carlo simulations. Based on the non-observation of WIMPs and using standard assumptions about the galactic halo [68], the 90% C.L. upper limit of the spin-independent WIMPnucleon cross section for the first 5 tower run is 6.6 × 10-44cm2 for a 60 GeV/c2 WIMP mass. A combined limit using all the data taken at Soudan results in an upper limit of 4.6×10-44cm2 at 90% C.L.for a 60 GeV/c2 WIMP mass. This new limit corresponds to a factor of ~3 improvement over any previous CDMS-II limit and a factor of ~2 above 60 GeV/c 2 better than any other WIMP search to date. This thesis presents an estimation, based on Monte Carlo simulations, of the nuclear recoils produced by cosmic-ray muons and their secondaries (at the Soudan site) for a 5 tower Ge and Si configuration as well as for a 7 supertower array. The results of the Monte Carlo are that CDMS-II should expect 0.06 ± 0.02+0.18 -0.02 /kgyear unvetoed single nuclear recoils in Ge for the 5 tower configuration, and 0.05 ± 0.01+0.15 -0.02 /kg-year for the 7 supertower configuration. The systematic error is based on the available

  8. Experimental Study on the Sensitive Emission Lines Intensities of Metal Samples Using Laser Ablation Technique and Its Comparison to Arc Discharge Technique

    Directory of Open Access Journals (Sweden)

    Eko Susilowati

    2004-05-01

    Full Text Available An experimental study has been carried out to measure the sensitive emission lines intensities of several metal samples (copper, zinc, silver, gold, gallium, nickel, silicone and iron using laser ablation technique conducted in low pressure surrounding gas by means of Laser Induced Shock Wave Plasma Spectroscopy (LISPS and in atmospheric pressure region using Laser Induced Breakdown Spectroscopy (LIBS. In both cases the Nd-YAG laser was operated at its fundamental wavelength of 1,064 nm with pulse duration of 8 ns and its intensity tightly focused on the metal samples in helium or air as an ambient gas. The laser energy was fixed at approximately 100 mJ using a set of neutral density filters placed tilted in front of the laser output window. The result of the intensity measurements showed a good agreement which those obtained using arc discharge technique as shown in Massachusetts Institute of Technology Wavelength Table. Further evaluation of these results on the basis of standard deviation leads to the conclusion that LISPS is more favorable for quantitative analysis compared to LIBS. It was further shown that replacing air by helium gas at low pressure improve to some extent the LISPS reproducibility and sensitivity.

  9. Transition between bulk and surface refractive index sensitivity of micro-cavity in-line Mach-Zehnder interferometer induced by thin film deposition.

    Science.gov (United States)

    Śmietana, Mateusz; Janik, Monika; Koba, Marcin; Bock, Wojtek J

    2017-10-16

    In this work we discuss the refractive index (RI) sensitivity of a micro-cavity in-line Mach-Zehnder interferometer in the form of a cylindrical hole (40-50 μm in diameter) fabricated in a standard single-mode optical fiber using a femtosecond laser. The surface of the micro-cavity was coated with up to 400 nm aluminum oxide thin film using the atomic layer deposition method. Next, the film was progressively chemically etched and the influence on changes in the RI of liquid in the micro-cavity was determined at different stages of the experiment, i.e., at different thicknesses of the film. An effect of transition between sensitivity to the film thickness (surface) and the RI of liquid in the cavity (bulk) is demonstrated for the first time. We have found that depending on the interferometer working conditions determined by thin film properties, the device can be used for investigation of phenomena taking place at the surface, such as in case of specific label-free biosensing applications, or for small-volume RI analysis as required in analytical chemistry.

  10. SU-E-T-159: Sensitivity of in Line Real Time Scintillating Fiber Detectors for External Beam Treatment Verification and Patient Safety.

    Science.gov (United States)

    Izaguirre, E W; Price, S G; Smajlovic, S; Yaddanapudi, S; Wooten, H; Mutic, S

    2012-06-01

    We studied the sensitivity of a novel transmission fiber scintillator array designed and built for in line treatment verification. The purpose of this project is to assess the capability of the fiber detector array technology to detect treatment errors in real time without false positives to enhance patient safety. We developed a linear scintillator array detector using radiation hard scintillating fibers and high speed parallel signal conditioning and data acquisition to monitor external beam treatment fluence in real time. The detector captures and resolves the time and amplitude of each linac pulse at each MLC segment. The detector has 60 fibers aligned to each MLC leaf and two output channels per fiber. The data is captured by a high speed parallel digitizer to determine the IMRT beam output delivered to a patient in real time. We evaluated the detector peak pulse linearity according to dose rate, MLC positioning, and beam energy. We analyzed the detector sensitivity, signal to noise ratio, and pulse distribution statistics to determine beam output and fluence in real time. We analyzed the response of the detector to 6 MV and 10 MV photon beams. The statistical analysis of the detected linac pulses indicates that a minimum of 20 pulses are required to evaluate MLC positioning and fluence with 3 mm and 3% resolution, respectively. During testing, no false positives were detected. Linearity with respect to output rate, MLC or jaw opening, and fluence is within 2%. Measured sensitivity and signal to noise ratio of a real time linear fiber array detector show that delivered beam fluence can be monitored every 55 msec, with no observed false positives during treatment to provide in vivo real time patient safety and beam monitoring. © 2012 American Association of Physicists in Medicine.

  11. Dark Kinetic Heating of Neutron Stars and an Infrared Window on WIMPs, SIMPs, and Pure Higgsinos.

    Science.gov (United States)

    Baryakhtar, Masha; Bramante, Joseph; Li, Shirley Weishi; Linden, Tim; Raj, Nirmal

    2017-09-29

    We identify a largely model-independent signature of dark matter (DM) interactions with nucleons and electrons. DM in the local galactic halo, gravitationally accelerated to over half the speed of light, scatters against and deposits kinetic energy into neutron stars, heating them to infrared blackbody temperatures. The resulting radiation could potentially be detected by the James Webb Space Telescope, the Thirty Meter Telescope, or the European Extremely Large Telescope. This mechanism also produces optical emission from neutron stars in the galactic bulge, and x-ray emission near the galactic center because dark matter is denser in these regions. For GeV-PeV mass dark matter, dark kinetic heating would initially unmask any spin-independent or spin-dependent dark matter-nucleon cross sections exceeding 2×10^{-45}  cm^{2}, with improved sensitivity after more telescope exposure. For lighter-than-GeV dark matter, cross-section sensitivity scales inversely with dark matter mass because of Pauli blocking; for heavier-than-PeV dark matter, it scales linearly with mass as a result of needing multiple scatters for capture. Future observations of dark sector-warmed neutron stars could determine whether dark matter annihilates in or only kinetically heats neutron stars. Because inelastic interstate transitions of up to a few GeV would occur in relativistic scattering against nucleons, elusive inelastic dark matter like pure Higgsinos can also be discovered.

  12. Dark Kinetic Heating of Neutron Stars and an Infrared Window on WIMPs, SIMPs, and Pure Higgsinos

    Science.gov (United States)

    Baryakhtar, Masha; Bramante, Joseph; Li, Shirley Weishi; Linden, Tim; Raj, Nirmal

    2017-09-01

    We identify a largely model-independent signature of dark matter (DM) interactions with nucleons and electrons. DM in the local galactic halo, gravitationally accelerated to over half the speed of light, scatters against and deposits kinetic energy into neutron stars, heating them to infrared blackbody temperatures. The resulting radiation could potentially be detected by the James Webb Space Telescope, the Thirty Meter Telescope, or the European Extremely Large Telescope. This mechanism also produces optical emission from neutron stars in the galactic bulge, and x-ray emission near the galactic center because dark matter is denser in these regions. For GeV-PeV mass dark matter, dark kinetic heating would initially unmask any spin-independent or spin-dependent dark matter-nucleon cross sections exceeding 2 ×10-45 cm2, with improved sensitivity after more telescope exposure. For lighter-than-GeV dark matter, cross-section sensitivity scales inversely with dark matter mass because of Pauli blocking; for heavier-than-PeV dark matter, it scales linearly with mass as a result of needing multiple scatters for capture. Future observations of dark sector-warmed neutron stars could determine whether dark matter annihilates in or only kinetically heats neutron stars. Because inelastic interstate transitions of up to a few GeV would occur in relativistic scattering against nucleons, elusive inelastic dark matter like pure Higgsinos can also be discovered.

  13. Upregulated expression of BCL2, MCM7, and CCNE1 indicate cisplatin-resistance in the set of two human bladder cancer cell lines: T24 cisplatin sensitive and T24R2 cisplatin resistant bladder cancer cell lines

    Directory of Open Access Journals (Sweden)

    Sung Han Kim

    2016-01-01

    Full Text Available Purpose: The mechanism of resistance to cisplatin during treatment of bladder cancer (BC has been a subject of intense investigation in clinical research. This study aims to identify candidate genes associated with resistance to cisplatin, in order to understand the resistance mechanism of BC cells to the drug, by combining the use of microarray profiling, quantitative reverse transcriptionpolymerase chain reaction (RT-PCR, and Western blot analyses. Materials and Methods: The cisplatin sensitive human BC cell line (T24 and the cisplatin resistant BC cell line, T24R2, were used for microarray analysis to determine the differential expression of genes that are significant in cisplatin resistance. Candidate upregulated genes belonging to three well-known cancer-related KEGG (Kyoto Encyclopedia of Genes and Genomes pathways (p53 tumor suppressor, apoptosis, and cell cycle were selected from the microarray data. These candidate genes, differentially expressed in T24 and T24R2, were then confirmed by quantitative RT-PCR and western blot. A fold change ≥2 with a p-value <0.05 was considered significant. Results: A total of 18 significantly upregulated genes were detected in the three selected cancer-related pathways in both microarray and RT-PCR analyses. These genes were PRKAR2A, PRKAR2B, CYCS, BCL2, BIRC3, DFFB, CASP6, CDK6, CCNE1, STEAP3, MCM7, ORC2, ORC5, ANAPC1 , and ANAPC7, CDC7, CDC27 , and SKP1 . Western blot analyses also confirmed the upregulation of BCL2, MCM7, and CCNE1 at the protein level, indicating their crucial association with cisplatin resistance. Conclusions: The BCL2, MCM7 , and CCNE1 genes might play distinctive roles in cisplatin resistance in BC.

  14. Two component WIMP-FImP dark matter model with singlet fermion, scalar and pseudo scalar

    Energy Technology Data Exchange (ETDEWEB)

    Dutta Banik, Amit; Pandey, Madhurima; Majumdar, Debasish [Saha Institute of Nuclear Physics, HBNI, Astroparticle Physics and Cosmology Division, Kolkata (India); Biswas, Anirban [Harish Chandra Research Institute, Allahabad (India)

    2017-10-15

    We explore a two component dark matter model with a fermion and a scalar. In this scenario the Standard Model (SM) is extended by a fermion, a scalar and an additional pseudo scalar. The fermionic component is assumed to have a global U(1){sub DM} and interacts with the pseudo scalar via Yukawa interaction while a Z{sub 2} symmetry is imposed on the other component - the scalar. These ensure the stability of both dark matter components. Although the Lagrangian of the present model is CP conserving, the CP symmetry breaks spontaneously when the pseudo scalar acquires a vacuum expectation value (VEV). The scalar component of the dark matter in the present model also develops a VEV on spontaneous breaking of the Z{sub 2} symmetry. Thus the various interactions of the dark sector and the SM sector occur through the mixing of the SM like Higgs boson, the pseudo scalar Higgs like boson and the singlet scalar boson. We show that the observed gamma ray excess from the Galactic Centre as well as the 3.55 keV X-ray line from Perseus, Andromeda etc. can be simultaneously explained in the present two component dark matter model and the dark matter self interaction is found to be an order of magnitude smaller than the upper limit estimated from the observational results. (orig.)

  15. Conformational properties of nickel(II) octaethyl porphyrin insolution. 1. Resonance excitation profiles and temperature dependence of structure-sensitive Raman lines

    Energy Technology Data Exchange (ETDEWEB)

    Jentzen, W. [Universitaet Bremen (Germany)]|[Sandia National Lab., Albuquerque, NM (United States); Unger, E.; Karvounis, G.; Dreybrodt, W.; Schweitzer-Stenner, R. [Universitaet Bremen (Germany); Shelnutt, J.A. [Sandia National Lab., Albuquerque, NM (United States)]|[Univ. of New Mexico, Albuquerque, NM (United States)

    1996-08-15

    We have measured polarized resonance Raman spectra of nickel(II)octaethylporphyrin in CS{sub 2} and CH{sub 2}Cl{sub 2} solution at different excitation wavelengths (430-580nm) and temperatures (190-130 K). The analysis of the spectra revealed that the structure-sensitive Raman lines v{sub 19} and v{sub 10} can be decomposed consistently into two sublines for all excitation wavelengths and temperatures. In the resonance region of the Q{sub O} and Q{sub V} bands, the 0-1 and 0-0 resonances in the excitation profiles of the low-frequency (LF) sublines of v{sub 19} and v{sub 10} are red-shifted by (150 30) cm{sup -1} with respect to the sublines that are at higher frequencies (HF). In accordance with experimental and theoretical results, this indicates that the LF sublines of v{sub 19} and v{sub 10} result from a nonplanar conformer, whereas the HF sublines correspond to an almost planar conformer. The existence of this known conformational equilibrium insolution is further corroborated by the van`t Hoff behavior of the intensity ratios I{sub LF}/I{sub HF} of the sublines of v{sub 19} and v{sub 10}. From the straight lines in van`t Hoff plot, we calculate that the nonplanar conformer in solution is energetically favored by about 3.0 kJ/mol. 28 refs., 7 figs., 2 tabs.

  16. Pre-launch Estimates for GLAST Sensitivity to Dark Matter Annihilation Signals

    Energy Technology Data Exchange (ETDEWEB)

    Baltz, E.A.; Berenji, B.; /SLAC /KIPAC, Menlo Park; Bertone, G.; /Paris, Inst. Astrophys.; Bergstrom, L.; /Stockholm U.; Bloom, E.; /SLAC /KIPAC, Menlo Park; Bringmann, T.; /Stockholm U.; Chiang, J.; Cohen-Tanugi, J.; /SLAC /KIPAC, Menlo Park; Conrad, J.; /Stockholm U.; Edmonds, Y.; /SLAC /KIPAC, Menlo Park; Edsjo, J.; /Stockholm U.; Godfrey, G.; /SLAC /KIPAC, Menlo Park; Hughes, R.E.; /Ohio State U.; Johnson, R.P.; /UC, Santa Cruz; Lionetto, A.; /Rome U.,Tor Vergata /INFN, Rome2; Moiseev, A.A.; /CRESST; Morselli, A.; /Rome U.,Tor Vergata /INFN, Rome2; Moskalenko, I.V.; /Stanford U., HEPL /KIPAC, Menlo Park; Nuss, E.; /Montpellier U.; Ormes, J.F.; /Denver U.; Rando, R.; /INFN, Padua /Ohio State U. /Stockholm U. /Ohio State U. /Garching, Max Planck Inst., MPE /SLAC /KIPAC, Menlo Park /Ohio State U.

    2009-05-15

    We investigate the sensitivity of the Gamma-ray Large Area Space Telescope (GLAST) to indirectly detect weakly interacting massive particles (WIMPs) through the {gamma}-ray signal that their pair annihilation produces. WIMPs are among the favorite candidates to explain the compelling evidence that about 80% of the mass in the Universe is non-baryonic dark matter (DM). They are serendipitously motivated by various extensions of the standard model of particle physics such as Supersymmetry and Universal Extra Dimensions (UED). With its unprecedented sensitivity and its very large energy range (20 MeV to more than 300 GeV) the main instrument on board the GLAST satellite, the Large Area Telescope (LAT), will open a new window of discovery. As our estimates show, the LAT will be able to detect an indirect DM signature for a large class of WIMP models given a cuspy profile for the DM distribution. Using the current state of the art Monte Carlo and event reconstruction software developed within the LAT collaboration, we present preliminary sensitivity studies for several possible sources inside and outside the Galaxy. We also discuss the potential of the LAT to detect UED via the electron/positron channel. Diffuse background modeling and other background issues that will be important in setting limits or seeing a signal are presented.

  17. Low dose high energy x-ray in-line phase sensitive imaging prototype: Investigation of optimal geometric conditions and design parameters.

    Science.gov (United States)

    Ghani, Muhammad U; Yan, Aimin; Wong, Molly D; Li, Yuhua; Ren, Liqiang; Wu, Xizeng; Liu, Hong

    2015-01-01

    The objective of this study was to investigate the optimization of a high energy in-line phase sensitive x-ray imaging prototype under different geometric and operating conditions for mammography application. A phase retrieval algorithm based on phase attenuation duality (PAD) was applied to the phase contrast images acquired by the prototype. Imaging performance was investigated at four magnification values of 1.67, 2, 2.5 and 3 using an acrylic edge, an American College of Radiology (ACR) mammography phantom and contrast detail (CD) phantom with tube potentials of 100, 120 and 140 kVp. The ACR and CD images were acquired at the same mean glandular dose (MGD) of 1.29 mGy with a computed radiography (CR) detector of 43.75 μm pixel pitch at a fixed source to image distance (SID) of 170 cm. The x-ray tube focal spot size was kept constant as 7 μm while a 2.5 mm thick aluminum (Al) filter was used for beam hardening. The performance of phase contrast and phase retrieved images were compared with computer simulations based on the relative phase contrast factor (RPF) at high x-ray energies. The imaging results showed that the x-ray tube operated at 100 kVp under the magnification of 2.5 exhibits superior imaging performance which is in accordance to the computer simulations. As compared to the phase contrast images, the phase retrieved images of the ACR and CD phantoms demonstrated improved imaging contrast and target discrimination. We compared the CD phantom images acquired in conventional contact mode with and without the anti-scatter grid using the same prototype at 1.295 mGy and 2.59 mGy using 40 kVp, a 25 μm rhodium (Rh) filter. At the same radiation dose, the phase sensitive images provided improved detection capabilities for both the large and small discs, while compared to the double dose image acquired in conventional mode, the observer study also indicated that the phase sensitive images provided improved detection capabilities for the large discs. This

  18. Pressure-assisted electrokinetic injection for on-line enrichment in capillary electrophoresis-mass spectrometry: a sensitive method for measurement of ten haloacetic acids in drinking water.

    Science.gov (United States)

    Zhang, Huijuan; Zhu, Jiping; Aranda-Rodriguez, Rocio; Feng, Yong-Lai

    2011-11-07

    Haloacetic acids (HAAs) are by-products of the chlorination of drinking water containing natural organic matter and bromide. A simple and sensitive method has been developed for determination of ten HAAs in drinking water. The pressure-assisted electrokinetic injection (PAEKI), an on-line enrichment technique, was employed to introduce the sample into a capillary electrophoresis (CE)-electrospray ionization-tandem mass spectrometry system (ESI-MS/MS). HAAs were monitored in selected reaction monitoring mode. With 3 min of PAEKI time, the ten major HAAs (HAA10) in drinking water were enriched up to 20,000-fold into the capillary without compromising resolution. A simple solid phase clean-up method has been developed to eliminate the influence of ionic matrices from drinking water on PAEKI. Under conditions optimized for mass spectrometry, PAEKI and capillary electrophoresis, detection limits defined as three times ratio of signal to noise have been achieved in a range of 0.013-0.12 μg L(-1) for ten HAAs in water sample. The overall recoveries for all ten HAAs in drinking water samples were between 76 and 125%. Six HAAs including monochloro- (MCAA), dichloro- (DCAA), trichloro- (TCAA), monobromo- (MBAA), bromochloro- (BCAA), and bromodichloroacetic acids (BDCAA) were found in tap water samples collected. Crown Copyright © 2011. Published by Elsevier B.V. All rights reserved.

  19. Investigating the Effect of Line Dipole Magnetic Field on Hydrothermal Characteristics of a Temperature-Sensitive Magnetic Nanofluid Using Two-Phase Simulation.

    Science.gov (United States)

    Bahiraei, Mehdi; Hangi, Morteza

    2016-12-01

    Hydrothermal characteristics of a temperature-sensitive magnetic nanofluid between two parallel plates are investigated in the presence of magnetic field produced by one or multiple line dipole(s) using the two-phase mixture model. As the nanofluid reaches the region where the magnetic field is applied, a rotation is developed due to the dependency of magnetization on temperature. This can lead to mixing in the flow and more uniform distribution of temperature due to the disturbance caused in the boundary layer, and consequently, enhancement in convective heat transfer. The results indicate that the disturbance in boundary layer adjacent to the lower wall is more significant than the upper wall. By application of the magnetic field, the convective heat transfer increases locally for both walls. Due to the intensified mixing, a sudden pressure drop occurs when the fluid reaches the region where the magnetic field is applied. For greater magnetic field strengths and lower Reynolds numbers, the improvement in convective heat transfer is more significant. For small magnetic field strengths, the effect of applying magnetic field on the upper wall is much smaller than that on the lower wall; however, this effect becomes almost the same for both walls at great magnetic field strengths.

  20. Detection of WIMPs in the Edelweiss experiment. Study of the radioactive background noise and measurements with bolometers; Recherche des wimp's du halo galactique dans l'experience edelweiss: etude du bas bruit radioactif et mesures a l'aide de bolometres a double detection ionisation/chaleur

    Energy Technology Data Exchange (ETDEWEB)

    Miramonti, L. [CEA/Saclay, Dept. d' Astrophysique, de la Physique des Particules, de la Physique Nucleaire et de l' Instrumentation Associee (DAPNIA), 91 - Gif-sur-Yvette (France)]|[Paris-11 Univ., 91 - Orsay (France)

    1999-11-01

    This thesis is dedicated to the detection of black matter in the form of WIMPs (weakly interacting massive particle). The characteristics of the interaction of WIMPs with matter are recalled. The very low number of expected events (<1 event/day.Kg) implies a radioactive background noise as weak as possible, furthermore the exponential decrease of the interacting rate with increasing energy calls for detectors with very low thresholds. Bolometers present advantages in WIMPs detection: i) very good resolution and very low thresholds, ii) very broad range of materials that can be used as absorber, the only requirements are: a crystal structure, to be a diamagnetic isolator and to have a convenient Debye temperature, iii) the possibility of detecting ionizing or non-ionizing particles, iv) the possibility of identifying the incident particle by measuring both temperature and another parameter such as ionization or scintillation, and v) the possibility of localizing the interaction inside the absorber by detecting ballistic phonons. The problematic of radioactive background noise is presented and the answers to the different sources (cosmic radiation,natural and artificial radioactivity) generating the background noise are examined. The materials used in the building of the detector and cryostat must be carefully chosen, they should be as little radioactive as possible. The test benches used to select materials for the Edelweiss experiment are described. The first measurements concerning the detectors Ge-4 and Ge-7 are presented. (A.C.)

  1. WMAP7 and future CMB constraints on annihilating dark matter: implications for GeV-scale WIMPs

    Science.gov (United States)

    Hütsi, G.; Chluba, J.; Hektor, A.; Raidal, M.

    2011-11-01

    Aims: We calculate constraints from current and future cosmic microwave background (CMB) measurements on annihilating dark matter (DM) with masses below the electroweak scale: mDM = 5 - 100 GeV. In particular, we assume the S-wave annihilation mode to be dominant, and focus our attention on the lower end of this mass range, as DM particles with masses mDM ~ 10 GeV have recently been claimed to be consistent with the CoGeNT and DAMA/LIBRA results, while also providing viable DM candidates to explain the measurements of Fermi and WMAP haze. We study the model (in)dependence of the CMB power spectra on particle physics DM models, large-scale structure formation and cosmological uncertainties. We attempt to find a simple and practical recipe for estimating current and future CMB bounds on a broad class of DM annihilation models. Methods: We use a model-independent description for DM annihilation into a wide set of Standard Model particles simulated by PYTHIA Monte Carlo. Our Markov chain Monte Carlo calculations used for finding model constraints involve realistic CMB likelihoods and assume a standard 6-parameter ΛCDM background cosmological model, which is extended by two additional DM annihilation parameters: mDM and ⟨ σAυ ⟩ /mDM. Results: We show that in the studied DM mass range the CMB signal of DM annihilations is independent of the details of large-scale structure formation, distribution, and profile of DM halos and other cosmological uncertainties. All particle physics models of DM annihilation can be described with only one parameter, the fraction of energy carried away by neutrinos in DM annihilation. As the main result we provide a simple and rather generic fitting formula for calculating CMB constraints on the annihilation cross section of light WIMPs. We show that thermal relic DM in the CoGeNT, DAMA/LIBRA favored mass range is in a serious conflict with present CMB data for the annihilation channels with few neutrinos, and will definitely be tested

  2. Chronic citalopram treatment elevates serotonin synthesis in Flinders Sensitive and Flinders Resistant Lines of rats, with no significant effect on Sprague-Dawley rats

    Science.gov (United States)

    Kanemaru, Kazuya; Nishi, Kyoko; Hasegawa, Shu; Diksic, Mirko

    2009-01-01

    The influence of citalopram on regional 5-hydroxytryptamine (serotonin, 5-HT) synthesis, one of the most important pre-synaptic parameters of serotonergic neurotransmission, was studied. Sprague-Dawley (SPD) rats were used as the controls, and Flinders Resistant Line (FRL) rats were used as auxiliary controls, to hopefully obtain a better understanding of the effects of citalopram on Flinders Sensitive Line (FSL; “depressed”) rats. Regional 5-HT synthesis was evaluated using a radiographic method with a labelled tryptophan analog tracer. In each strain of rats, the animals were treated with citalopram (10 mg/kg/day) or saline for fourteen days. The groups consisted of between fourteen and twenty rats. There were six groups of rats with citalopram (CIT) and saline (SAL) groups in each of the strains (SPD-SAL, SPD-CIT, FRLSAL, FRL-CIT, FSL-SAL and FSL-CIT). A two-factor analysis of variance was used to evaluate the effect of the treatment (e.g., SPD-SAL relative to SPD-CIT) followed by planned comparisons to evaluate the effect in each brain region. In addition, the planned comparison with appropriate contrast was used to evaluate a relative effects in SPD relative to FSL and FRL, and FSL relative to FRL groups. A statistical analysis was first performed in the apriori selected regions, because we had learned, from previous work, that it was possible to select the brain regions in which neurochemical variables had been altered by the disorder and subsequent antidepressant treatments. The results clearly show that citalopram treatment does not have an overall effect on synthesis in the control SPD rats; there was no significant (p>0.05) difference between the SPD-SAL and SPD-CIT rats. In “depressed” FSL rats, citalopram produced a significant (papriori selected brain regions, while in the SPD rats, citalopram produced significant changes in only four out of seventeen apriori selected regions. The statistical evaluation also revealed that changes produced by

  3. Use of a murine embryonic stem cell line that is sensitive to high glucose environment to model neural tube development in diabetic pregnancy.

    Science.gov (United States)

    Sanders, Kaitlyn; Jung, Jin Hyuk; Loeken, Mary R

    2014-08-01

    Neural tube defects (NTDs) are significantly increased by maternal diabetes. Embryonic stem cells (ESC) that can differentiate into neuroepithelium and can sense supraphysiological glucose concentrations would be very valuable to simulate the effects of maternal diabetes on molecular and cellular processes during neural tube formation. LG-ESC, a recently established ESC line that expresses the glucose transporter, Scl2a2, and is sensitive to elevated glucose concentrations, were grown for up to 8 days in a three-dimensional culture to form neural cysts. We tested whether high glucose media inhibits expression of Pax3, a gene that is required for neural tube closure and whose expression is inhibited in embryos of diabetic mice, and inhibits formation of neural cysts. Pax3 expression was detected after 4 days of culture and increased with time. Pax3 expression was inhibited by high glucose media, but not if cells had been cultured in low glucose media for the first 4 days of culture. Pax7, which is also expressed in dorsal neural tube, was not detected. Pax6, which is expressed in the ventral neural tube, was detected only after 8 days of culture, but was not inhibited by high glucose. High glucose media did not inhibit formation of neural cysts. LG-ESC can be used as a model of embryonic exposure to a diabetic environment during neural tube development. While high glucose exposure inhibits expression of a gene required for neural tube closure, it may not inhibit all of the processes involved in formation of a neural tube-like structure. © 2014 Wiley Periodicals, Inc.

  4. In-Line Ozonation for Sensitive Air-Monitoring of a Mustard-Gas Simulant by Atmospheric Pressure Chemical Ionization Mass Spectrometry

    Science.gov (United States)

    Okumura, Akihiko

    2015-09-01

    A highly sensitive method for real-time air-monitoring of mustard gas (bis(2-chloroethyl) sulfide, HD), which is a lethal blister agent, is proposed. Humidified air containing a HD simulant, 2-chloroethyl ethyl sulfide (2CEES), was mixed with ozone and then analyzed by using an atmospheric pressure chemical ionization ion trap tandem mass spectrometer. Mass-spectral ion peaks attributable to protonated molecules of intact, monooxygenated, and dioxygenated 2CEES (MH+, MOH+, and MO2H+, respectively) were observed. As ozone concentration was increased from zero to 30 ppm, the signal intensity of MH+ sharply decreased, that of MOH+ increased once and then decreased, and that of MO2H+ sharply increased until reaching a plateau. The signal intensity of MO2H+ at the plateau was 40 times higher than that of MH+ and 100 times higher than that of MOH+ in the case without in-line ozonation. Twenty-ppm ozone gas was adequate to give a linear calibration curve for 2CEES obtained by detecting the MO2H+ signal in the concentration range up to 60 μg/m3, which is high enough for hygiene management. In the low concentration range lower than 3 μg/m3, which is equal to the short-term exposure limit for HD, calibration plots unexpectedly fell off the linear calibration curve, but 0.6-μg/m3 vapor was actually detected with the signal-to-noise ratio of nine. Ozone was generated from instrumentation air by using a simple and inexpensive home-made generator. 2CEES was ozonated in 1-m extended sampling tube in only 1 s.

  5. Comparision of the Cytotoxic Effects of Birch Bark Extract, Betulin and Betulinic Acid Towards Human Gastric Carcinoma and Pancreatic Carcinoma Drug-sensitive and Drug-Resistant Cell Lines

    Directory of Open Access Journals (Sweden)

    Hermann Lage

    2009-04-01

    Full Text Available Betulin and betulinic acid are naturally occurring pentacyclic triterpenes showing cytotoxicity towards a number of cancer cell lines. These compounds can be found in the bark of the many plants. In this report we have compared the cytotoxic activity of crude birch bark extract and purified betulin and betulinic acid towards human gastric carcinoma (EPG85-257 and human pancreatic carcinoma (EPP85-181 drug-sensitive and drug-resistant (daunorubicin and mitoxantrone cell lines. Our results show significant differences in sensitivity between cell lines depending on the compound used, and suggest that both betulin and betulinic acid can be considered as a promising leads in the treatment of cancer.

  6. Response to mTOR inhibition: activity of eIF4E predicts sensitivity in cell lines and acquired changes in eIF4E regulation in breast cancer

    Directory of Open Access Journals (Sweden)

    Bartlett John MS

    2011-02-01

    Full Text Available Abstract Background Inhibitors of the kinase mTOR, such as rapamycin and everolimus, have been used as cancer therapeutics with limited success since some tumours are resistant. Efforts to establish predictive markers to allow selection of patients with tumours likely to respond have centred on determining phosphorylation states of mTOR or its targets 4E-BP1 and S6K in cancer cells. In an alternative approach we estimated eIF4E activity, a key effector of mTOR function, and tested the hypothesis that eIF4E activity predicts sensitivity to mTOR inhibition in cell lines and in breast tumours. Results We found a greater than three fold difference in sensitivity of representative colon, lung and breast cell lines to rapamycin. Using an assay to quantify influences of eIF4E on the translational efficiency specified by structured 5'UTRs, we showed that this estimate of eIF4E activity was a significant predictor of rapamycin sensitivity, with higher eIF4E activities indicative of enhanced sensitivity. Surprisingly, non-transformed cell lines were not less sensitive to rapamycin and did not have lower eIF4E activities than cancer lines, suggesting the mTOR/4E-BP1/eIF4E axis is deregulated in these non-transformed cells. In the context of clinical breast cancers, we estimated eIF4E activity by analysing expression of eIF4E and its functional regulators within tumour cells and combining these scores to reflect inhibitory and activating influences on eIF4E. Estimates of eIF4E activity in cancer biopsies taken at diagnosis did not predict sensitivity to 11-14 days of pre-operative everolimus treatment, as assessed by change in tumour cell proliferation from diagnosis to surgical excision. However, higher pre-treatment eIF4E activity was significantly associated with dramatic post-treatment changes in expression of eIF4E and 4E-binding proteins, suggesting that eIF4E is further deregulated in these tumours in response to mTOR inhibition. Conclusions

  7. Veliparib Monotherapy to Patients With BRCA Germ Line Mutation and Platinum-Resistant or Partially Platinum-Sensitive Relapse of Epithelial Ovarian Cancer

    DEFF Research Database (Denmark)

    Steffensen, Karina Dahl; Adimi, Parvin; Jakobsen, Anders

    2017-01-01

    defects in the BRCA genes are particularly sensitive to treatment with poly(ADP-ribose) polymerase inhibitors. The main purpose of this study was to investigate the effect of veliparib in patients with known BRCA1/2 mutations and with a platinum-resistant or intermediate sensitive relapse of OC. METHODS...

  8. Genome-wide transcriptomic analysis of response to low temperature reveals candidate genes determining divergent cold-sensitivity of maize inbred lines

    OpenAIRE

    Sobkowiak, Alicja; Jończyk, Maciej; Jarochowska, Emilia; Biecek, Przemysław; Trzcinska-Danielewicz, Joanna; Leipner, Jörg; Fronk, Jan; Sowiński, Paweł

    2014-01-01

    Maize, despite being thermophyllic due to its tropical origin, demonstrates high intraspecific diversity in cold-tolerance. To search for molecular mechanisms of this diversity, transcriptomic response to cold was studied in two inbred lines of contrasting cold-tolerance. Microarray analysis was followed by extensive statistical elaboration of data, literature data mining, and gene ontology-based classification. The lines used had been bred earlier specifically for determination of QTLs for c...

  9. rIFN-γ-mediated growth suppression of platinum-sensitive and -resistant ovarian tumor cell lines not dependent upon arginase inhibition

    Directory of Open Access Journals (Sweden)

    Gallardo Stacie T

    2003-09-01

    Full Text Available Abstract Background Arginine metabolism in tumor cell lines can be influenced by various cytokines, including recombinant human interferon-γ (rIFN-γ, a cytokine that shows promising clinical activity in epithelial ovarian cancer (EOC. Methods We examined EOC cell lines for the expression of arginase in an enzymatic assay and for transcripts of arginase I and II, inducible nitric oxide synthase (iNOS, and indoleamine 2,3-dioxygenase (IDO by reverse transcription-polymerase chain reaction. The effects of rIFN-γ on arginase activity and on tumor cell growth inhibition were determined by measuring [3H]thymidine uptake. Results Elevated arginase activity was detected in 5 of 8 tumor cell lines, and analysis at the transcriptional level showed that arginase II was involved but arginase I was not. rIFN-γ reduced arginase activity in 3 EOC cell lines but increased activity in the 2008 cell line and its platinum-resistant subline, 2008.C13. iNOS transcripts were not detected in rIFN-γ-treated or untreated cell lines. In contrast, IDO activity was induced or increased by rIFN-γ. Suppression of arginase activity by rIFN-γ in certain cell lines suggested that such inhibition might contribute to its antiproliferative effects. However, supplementation of the medium with polyamine pathway products did not interfere with the growth-inhibitory effects of rIFN-γ EOC cells. Conclusions Increased arginase activity, specifically identified with arginase II, is present in most of the tested EOC cell lines. rIFN-γ inhibits or stimulates arginase activity in certain EOC cell lines, though the decrease in arginase activity does not appear to be associated with the in vitro antiproliferative activity of rIFN-γ. Since cells within the stroma of EOC tissues could also contribute to arginine metabolism following treatment with rIFN-γ or rIFN-γ-inducers, it would be helpful to examine these effects in vivo.

  10. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Puget Sound and Strait of Juan de Fuca, Washington: FISHL (Fish Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for marine, estuarine, and anadromous fish in Puget Sound and Strait of Juan de Fuca, Washington. Vector...

  11. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Puget Sound and Strait of Juan de Fuca, Washington: SOCECON (Socioeconomic Resource Points and Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains points that represent the following sensitive human-use socioeconomic sites in Puget Sound and the Strait of Juan de Fuca, Washington: access...

  12. Silicon photonic crystal microarrays for high throughput label-free detection of lung cancer cell line lysates with sensitivity and specificity

    Science.gov (United States)

    Chakravarty, Swapnajit; Lai, Wei-Cheng; Zou, Yi; Gemmill, Robert M.; Chen, Ray T.

    2013-03-01

    Detection of biomolecules on microarrays based on label-free on-chip optical biosensors is very attractive since this format avoids complex chemistries caused by steric hindrance of labels. Application areas include the detection of cancers and allergens, and food-borne pathogens to name a few. We have demonstrated photonic crystal microcavity biosensors with high sensitivity down to 1pM concentrations (67pg/ml). High sensitivities were achieved by slow light engineering which reduced the radiation loss and increased the stored energy in the photonic crystal microcavity resonance mode. Resonances with high quality factor Q~26,760 in liquid ambient, coupled with larger optical mode volumes allowed enhanced interaction with the analyte biomolecules which resulted in sensitivities down to 10 cells per micro-liter to lung cancer cell lysates. The specificity of detection was ensured by multiplexed detections from multiple photonic crystal microcavities arrayed on the arms of a multimode interference power splitter. Specific binding interactions and control experiments were performed simultaneously at the same instant of time with the same 60 microliter sample volume. Specificity is further ensured by sandwich assay methods in the multiplexed experiment. Sandwich assay based amplification increased the sensitivity further resulting in the detection of lung cancer cell lysates down to concentrations of 2 cells per micro-liter. The miniaturization enabled by photonic crystal biosensors coupled with waveguide interconnected layout thus offers the potential of high throughput proteomics with high sensitivity and specificity.

  13. TIMP-1 overexpression does not affect sensitivity to HER2-targeting drugs in the HER2-gene-amplified SK-BR-3 human breast cancer cell line

    DEFF Research Database (Denmark)

    Deng, Xiaohong; Fogh, Louise; Lademann, Ulrik Axel

    2013-01-01

    Tissue inhibitor of metalloproteinases-1 (TIMP-1) has been suggested as a marker of prognosis and response to treatment in breast cancer. In vitro, TIMP-1 can regulate shedding of the extracellular domain of HER2 and signalling via the Akt pathway, and we hypothesize that TIMP-1 therefore can...... affect sensitivity to the HER2-targeting drugs trastuzumab and lapatinib. SK-BR-3 human breast cancer cells were stably transfected with TIMP-1, characterized with regard to TIMP-1 protein expression, proliferation, and functionality of the secreted TIMP-1, and the sensitivity to trastuzumab...... of TIMP-1 protein. Western blotting showed that the activation of Akt, PTEN, and HER2 as well as ADAM10 was similar in all clones. In conclusion, in this model, TIMP-1 overexpression does not affect HER2 cleavage by ADAM10 or signalling via the Akt pathway, and TIMP-1 does not influence sensitivity...

  14. Efficacy and safety of endocrine monotherapy as first-line treatment for hormone-sensitive advanced breast cancer: A network meta-analysis.

    Science.gov (United States)

    Zhang, Jingwen; Huang, Yanhong; Wang, Changyi; He, Yuanfang; Zheng, Shukai; Wu, Kusheng

    2017-08-01

    Endocrine therapy was recommended as the preferred first-line treatment for hormone receptor-positive (HR+, i.e., ER+ and/or PgR+), human epidermal growth factor receptor-2-negative (HER2-) postmenopausal advanced breast cancer (ABC), but which endocrine monotherapy is optimal lacks consensus. We aimed to identify the optimal endocrine monotherapy with a network meta-analysis. We performed a network meta-analysis for a comprehensive analysis of 6 first-line endocrine monotherapies (letrozole, anastrozole, exemestane, tamoxifen, fulvestrant 250 mg and 500 mg) for HR+ HER2- metastatic or locally advanced breast cancer in postmenopausal patients. The main outcomes were objective response rate (ORR), time to progression (TTP), and progression-free survival (PFS). Secondary outcomes were adverse events. We identified 27 articles of 8 randomized controlled trials including 3492 patients in the network meta-analysis. For ORR, the treatments ranked in descending order of effectiveness were letrozole > exemestane > anastrozole > fulvestrant 500 mg > tamoxifen > fulvestrant 250 mg. For TTP/PFS, the order was fulvestrant 500 mg > letrozole > anastrozole > exemestane > tamoxifen > fulvestrant 250 mg. We directly compared adverse events and found that tamoxifen produced more hot flash events than fulvestrant 250 mg. Fulvestrant 500 mg and letrozole might be optimal first-line endocrine monotherapy choices for HR+ HER2- ABC because of efficacious ORR and TTP/PFS, with a favorable tolerability profile. However, direct comparisons among endocrine monotherapies in the first-line therapy setting are still required to robustly demonstrate any differences among these endocrine agents. Clinical choices should also depend on the specific disease situation and duration of endocrine therapy.

  15. RC-IAL cell line: sensitivity of rubella virus grow Linhagem celular RC-IAL: sensibilidade de crescimento do vírus da rubéola

    Directory of Open Access Journals (Sweden)

    Cristina A Figueiredo

    2000-08-01

    Full Text Available OBJECTIVE: The rapid growth of the rubella virus in RC-IAL² with development of cytopathic effect, in response to rubella virus infection, is described. For purposes of comparison, the rubella virus RA-27/3 strain was titered simultaneously in the RC-IAL, Vero, SIRC and RK13 cell lines. METHODS: Rubella virus RA-27/3 strain are inoculated in the RC-IAL cell line (rabbit Kidney, Institute Adolfo Lutz. Plates containing 1.5x10(5 cells/ml of RC-IAL line were inoculated with 0.1ml s RA-27/3 strain virus containing 1x 10(4TCID50/0.1ml. A 25% cytopathic effect was observed after 48 hours and 100% after 96 hours. The results obtained were compared to those observed with the SIRC, Vero and RK13 cell lines. Rubella virus was detected by immunohistochemistry. RESULTS: With the results, it was possible to conclude that the RC-IAL cell line is a very good substrate for culturing rubella virus. The cells inoculated with rubella virus were examined by phase contrast microscopy and showed the characteristic rounded, bipolar and multipolar cells. The CPE in RC-IAL was observed in the first 48 hours and the curve of the increased infectivity was practically the same as observed in other cell lines. CONCLUSIONS: These findings are important since this is one the few cell lines described in the literature with a cytopathic effect. So it can be used for antigen preparation and serological testing for the diagnosis of specific rubella antibodies.OBJETIVO: Descreve-se o crescimento do vírus-padrão da rubéola RA-27/3 na linhagem celular RC-IAL, com desenvolvimento de efeito citopático em resposta à infecção viral. Para este propósito, o vírus-padrão foi titulado simultaneamente nas linhagens celulares Vero, SIRC e RK13. MÉTODOS: O vírus-padrão da rubéola RA-27/3 foi inoculado na linhagem celular RC-IAL (rim de coelho, Instituto Adolfo Lutz. Placas contendo 1,5x10(5 células/ml foram inoculadas com 0,1 ml do vírus contendo 1x10(4 DICT50/0,1 ml. O efeito

  16. Maturation sensitive and resistant t(15;17) NB4 cell lines as tools for APL physiopathology: nomenclature of cells and repertory of their known genetic alterations and phenotypes.

    Science.gov (United States)

    Roussel, M J; Lanotte, M

    2001-10-29

    Chromosomal translocations, leading to gene rearrangements that generate chimerical proteins, represent one of the initiating events of leukemia. Preleukemia cells eventually develop into overt leukemia by occurrence of secondary genetic alterations (tumor progression). The physiopathology of leukemia has made considerable progress during the last two decades, due to molecular biology investigations on the role played by the altered genes, during neoplasic hemopoiesis. In vitro studies have been facilitated by the establishment of stable leukemia cell lines bearing these gene rearrangements and secondary gene mutations. Investigations on acute promyelocytic leukemia (APL) have benefited from maturation sensitive and resistant cell lines (NB4 and UF-1) derived from APL patient's leukemia cells and bearing the t(15;17). The information concerning the NB4 cell line (responsiveness to retinoid/rexinoid, cAMP, arsenic, mutations causing resistance) is spread in an abundant literature. In this paper, we briefly recapitulate the cellular and molecular features of this cell line and its subclones with the aim of facilitating investigators in their choice of the most appropriate tool for their studies. As redundancy of several names given to NB4 sublines has sometimes created difficulties, we propose a nomenclature for the various NB4 sublines that most investigators certainly would be agreed with.

  17. Acquired TGF beta 1 sensitivity and TGF beta 1 expression in cell lines established from a single small cell lung cancer patient during clinical progression

    DEFF Research Database (Denmark)

    Nørgaard, P; Damstrup, L; Rygaard, K

    1996-01-01

    Three small cell lung cancer cell lines established from a single patient during longitudinal follow-up were examined for in vitro expression of TGF beta and TGF beta receptors, i.e. the components of an autocrine loop. GLC 14 was established prior to treatment, GLC 16 on relapse after chemotherapy...... and GLC 19 on recurrence after radiotherapy. TGF beta was detected by ELISA and TGF beta receptors by chemical crosslinking to radiolabelled TGF beta 1. Furthermore, TGF beta and TGF beta receptor mRNAs were detected by northern blot analysis. Expression of type II TGF beta receptor mRNA and protein...

  18. Genetic inactivation of the Fanconi anemia gene FANCC identified in the hepatocellular carcinoma cell line HuH-7 confers sensitivity towards DNA-interstrand crosslinking agents

    Directory of Open Access Journals (Sweden)

    Bassermann Florian

    2010-05-01

    Full Text Available Abstract Background Inactivation of the Fanconi anemia (FA pathway through defects in one of 13 FA genes occurs at low frequency in various solid cancer entities among the general population. As FA pathway inactivation confers a distinct hypersensitivity towards DNA interstrand-crosslinking (ICL-agents, FA defects represent rational targets for individualized therapeutic strategies. Except for pancreatic cancer, however, the prevalence of FA defects in gastrointestinal (GI tumors has not yet been systematically explored. Results A panel of GI cancer cell lines was screened for FA pathway inactivation applying FANCD2 monoubiquitination and FANCD2/RAD51 nuclear focus formation and a newly identified FA pathway-deficient cell line was functionally characterized. The hepatocellular carcinoma (HCC line HuH-7 was defective in FANCD2 monoubiquitination and FANCD2 nuclear focus formation but proficient in RAD51 focus formation. Gene complementation studies revealed that this proximal FA pathway inactivation was attributable to defective FANCC function in HuH-7 cells. Accordingly, a homozygous inactivating FANCC nonsense mutation (c.553C > T, p.R185X was identified in HuH-7, resulting in partial transcriptional skipping of exon 6 and leading to the classic cellular FA hypersensitivity phenotype; HuH-7 cells exhibited a strongly reduced proliferation rate and a pronounced G2 cell cycle arrest at distinctly lower concentrations of ICL-agents than a panel of non-isogenic, FA pathway-proficient HCC cell lines. Upon retroviral transduction of HuH-7 cells with FANCC cDNA, FA pathway functions were restored and ICL-hypersensitivity abrogated. Analyses of 18 surgical HCC specimens yielded no further examples for genetic or epigenetic inactivation of FANCC, FANCF, or FANCG in HCC, suggesting a low prevalence of proximal FA pathway inactivation in this tumor type. Conclusions As the majority of HCC are chemoresistant, assessment of FA pathway function in HCC could

  19. Lined canvas paintings

    DEFF Research Database (Denmark)

    Krarup Andersen, Cecil

    2013-01-01

    This work evaluates the lining history of the Danish Golden Age collection of canvas paintings from the nineteenths century at SMK, The National Gallery of Denmark, and seeks to understand the moisture sensitivity of the paintings before and after lining. This is done by chemical analysis...... and tensile testing samples from the tacking edges as well as lined mock-up samples. The ability of the different lining techniques to support canvas paintings is evaluated in terms of their initial stretching and during fluctuations of relative humidity. Six different combinations of lining adhesives...... and textiles were examined. These included the adhesives Beva 371, Plextol D360, wax-resin, and glue-paste. The lining textiles were linen and polyester sailcloth. A naturally aged canvas painting and a modern painting were lined using these materials and methods. Testing of the lined paintings included uni...

  20. Development of an artificial neural network model for risk assessment of skin sensitization using human cell line activation test, direct peptide reactivity assay, KeratinoSens™ and in silico structure alert parameter.

    Science.gov (United States)

    Hirota, Morihiko; Ashikaga, Takao; Kouzuki, Hirokazu

    2017-12-10

    It is important to predict the potential of cosmetic ingredients to cause skin sensitization, and in accordance with the European Union cosmetic directive for the replacement of animal tests, several in vitro tests based on the adverse outcome pathway have been developed for hazard identification, such as the direct peptide reactivity assay, KeratinoSens™ and the human cell line activation test. Here, we describe the development of an artificial neural network (ANN) prediction model for skin sensitization risk assessment based on the integrated testing strategy concept, using direct peptide reactivity assay, KeratinoSens™, human cell line activation test and an in silico or structure alert parameter. We first investigated the relationship between published murine local lymph node assay EC3 values, which represent skin sensitization potency, and in vitro test results using a panel of about 134 chemicals for which all the required data were available. Predictions based on ANN analysis using combinations of parameters from all three in vitro tests showed a good correlation with local lymph node assay EC3 values. However, when the ANN model was applied to a testing set of 28 chemicals that had not been included in the training set, predicted EC3s were overestimated for some chemicals. Incorporation of an additional in silico or structure alert descriptor (obtained with TIMES-M or Toxtree software) in the ANN model improved the results. Our findings suggest that the ANN model based on the integrated testing strategy concept could be useful for evaluating the skin sensitization potential. Copyright © 2017 John Wiley & Sons, Ltd.

  1. A new sensitive indicator cell line reveals cross-transactivation of the viral LTR by gorilla and chimpanzee simian foamy viruses.

    Science.gov (United States)

    Lambert, Caroline; Rua, Réjane; Gessain, Antoine; Buseyne, Florence

    2016-09-01

    The majority of currently identified simian foamy virus (SFV)-infected Cameroonian and Gabonese individuals harbor SFV from the gorilla lineage. We constructed an indicator cell line for the quantification of gorilla SFVs, in which the U3 sequence of a gorilla SFV directs the expression of the β-galactosidase protein. The gorilla foamy virus activated β-galactosidase (GFAB) cells efficiently quantified two zoonotic primary gorilla isolates and SFVs from three chimpanzee subspecies. Primary gorilla SFVs replicated more slowly and at lower levels than primary chimpanzee SFVs. Analysis of previously described motifs of Tas proteins and U3 LTRs involved in viral gene synthesis revealed conservation of such motifs in Tas proteins from gorilla and chimpanzee SFVs, but little sequence homology in the LTR regions previously shown to interact with viral and cellular factors. Copyright © 2016 Elsevier Inc. All rights reserved.

  2. On-line complexation/cloud point preconcentration for the sensitive determination of dysprosium in urine by flow injection inductively coupled plasma-optical emission spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Ortega, Claudia; Cerutti, Soledad; Silva, Maria F. [Departamento de Quimica Analitica, Facultad de Quimica, Bioquimica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, 5700, San Luis (Argentina); Olsina, Roberto A.; Martinez, Luis D. [Departamento de Quimica Analitica, Facultad de Quimica, Bioquimica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, 5700, San Luis (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Avda. Rivadavia 1917, CP C1033AAJJ, Buenos Aires (Argentina)

    2003-01-01

    An on-line dysprosium preconcentration and determination system based on the hyphenation of cloud point extraction (CPE) to flow injection analysis (FIA) associated with ICP-OES was studied. For the preconcentration of dysprosium, a Dy(III)-2-(5-bromo-2-pyridylazo)-5-diethylaminophenol complex was formed on-line at pH 9.22 in the presence of nonionic micelles of PONPE-7.5. The micellar system containing the complex was thermostated at 30 C in order to promote phase separation, and the surfactant-rich phase was retained in a microcolumn packed with cotton at pH 9.2. The surfactant-rich phase was eluted with 4 mol L{sup -1} nitric acid at a flow rate of 1.5 mL min{sup -1}, directly in the nebulizer of the plasma. An enhancement factor of 50 was obtained for the preconcentration of 50 mL of sample solution. The detection limit value for the preconcentration of 50 mL of aqueous solution of Dy was 0.03 {mu}g L{sup -1}. The precision for 10 replicate determinations at the 2.0 {mu}g L{sup -1}Dy level was 2.2% relative standard deviation (RSD), calculated from the peak heights obtained. The calibration graph using the preconcentration system for dysprosium was linear with a correlation coefficient of 0.9994 at levels near the detection limits up to at least 100 {mu}g L {sup -1}. The method was successfully applied to the determination of dysprosium in urine. (orig.)

  3. Conditionally immortal ovarian cell lines for investigating the influence of ovarian stroma on the estrogen sensitivity and tumorigenicity of ovarian surface epithelial cells.

    Science.gov (United States)

    Jiang, Feng; Saunders, Beatriz O; Haller, Edward; Livingston, Sandra; Nicosia, Santo V; Bai, Wenlong

    2003-01-01

    The tendency of the ovarian surface epithelium (OSE) to undergo metaplastic and morphogenetic changes during the life cycle, at variance with the adjacent peritoneal mesothelial cells, suggests that its biology may be regulated by underlying ovarian stromal cues. However, little is known about the role that the ovarian stroma plays in the pathobiology of the OSE, largely because of the lack of a suitable in vitro model. Here, we describe the establishment and characterization of conditionally immortalized ovarian stromal and surface epithelial cell lines from H-2K(b)-tsA58 transgenic mice that carry the thermolabile mutant of SV-40 large T antigen under the control of an interferon-gamma (IFN-gamma)-inducible promoter. These cells express functional T antigens, grow continuously under permissive conditions at 33 degrees C in the presence of IFN-gamma, and stop dividing when the activity and expression of the tumor antigen is suppressed by restrictive conditions without IFN-gamma at 39 degrees C. Morphological, immunohistochemical, and ultrastructural analyses show that conditionally immortal OSE cells form cobblestone-like monolayers, express cytokeratin and vimentin, contain several microvilli, and develop tight junctions, whereas stromal cells are spindle-like, express vimentin but not cytokeratin, and contain rare microvilli, thus exhibiting epithelial and stromal phenotypes, respectively. At variance with the reported behavior of rat epithelial cells, conditionally immortal mouse epithelial cells are not spontaneously transformed after continuous culture in vitro. More importantly, conditioned media from stromal cells cultured under permissive conditions increase the specific activity of the endogenous estrogen receptor in BG-1 human ovarian epithelial cancer cells and promote these cells' anchorage-independent growth, suggesting the paracrine influence of a stromal factor. In addition, stromal cells cultured under restrictive conditions retain this growth

  4. High-sensitivity HLA class I peptidome analysis enables a precise definition of peptide motifs and the identification of peptides from cell lines and patients' sera.

    Science.gov (United States)

    Ritz, Danilo; Gloger, Andreas; Weide, Benjamin; Garbe, Claus; Neri, Dario; Fugmann, Tim

    2016-05-01

    The characterization of peptides bound to human leukocyte antigen (HLA) class I is of fundamental importance for understanding CD8+ T cell-driven immunological processes and for the development of immunomodulatory therapeutic strategies. However, until now, the mass spectrometric analysis of HLA-bound peptides has typically required billions of cells, still resulting in relatively few high-confidence peptide identifications. Capitalizing on the recent developments in mass spectrometry and bioinformatics, we have implemented a methodology for the efficient recovery of acid-eluted HLA peptides after purification with the pan-reactive antibody W6/32 and have identified a total of 27 862 unique peptides with high confidence (1% false discovery rate) from five human cancer cell lines. More than 93% of the identified peptides were eight to 11 amino acids in length and contained signatures that were in excellent agreement with published HLA binding motifs. Furthermore, by purifying soluble HLA class I complexes (sHLA) from sera of melanoma patients, up to 972 high-confidence peptides could be identified, including melanoma-associated antigens already described in the literature. Knowledge of the HLA class I peptidome should facilitate multiplex tetramer technology-based characterization of T cells, and allow the development of patient selection, stratification and immunomodulatory therapeutic strategies. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  5. Rapid and sensitive on-line monitoring 6 different kinds of volatile organic compounds in aqueous samples by spray inlet proton transfer reaction mass spectrometry (SI-PTR-MS).

    Science.gov (United States)

    Zou, Xue; Kang, Meng; Wang, Hongmei; Huang, Chaoqun; Shen, Chengyin; Chu, Yannan

    2017-06-01

    Rapid and sensitive monitoring of volatile organic compounds (VOCs) in aqueous samples is very important to human health and environmental protection. In this study, an on-line spray inlet proton transfer reaction mass spectrometry (SI-PTR-MS) method was developed for the rapid and sensitive monitoring of 6 different kinds of VOCs, namely acetonitrile, acetaldehyde, ethanol, acetone, aether, and methylbenzene, in aqueous samples. The response time, limit of detection (LOD), and repeatability of the SI-PTR-MS system were evaluated. The response of the SI-PTR-MS was quite rapid with response times of 31-88 s. The LODs for all these VOCs were below 10 μg/L. The LOD of methylbenzene was 0.9 μg/L, much lower than the maximum contaminant level (MCL) in drinking water. The repeatability of this method was evaluated with 5 replicate determinations. The relative standard deviations (RSDs) were in the range of 0.8-3.1%, indicating good repeatability. To evaluate the matrix effects, the SI-PTR-MS system was employed for on-line monitoring of these 6 VOCs in different aqueous matrices, including lake water, tap water, and waste water. The relative recoveries were in the range of 94.6-106.0% for the lake water, 96.3-105.6% for the tap water, and 95.6-102.9% for the waste water. The results indicate that the SI-PTR-MS method has important application values in the rapid and sensitive monitoring of VOCs in these aqueous samples. In addition, the effect of salt concentration on the extracting efficiency was evaluated. The results showed that the LOD of the SI-PTR-MS could be further decreased by changing the salt concentration. Copyright © 2017 Elsevier Ltd. All rights reserved.

  6. Genetic resistance to Salmonella typhimurium in two lines of chickens selected as resistant and sensitive on the basis of heterophil/lymphocyte ratio.

    Science.gov (United States)

    Al-Murrani, W K; Al-Rawi, I K; Raof, N M

    2002-09-01

    1. A study was conducted to test for the validity of the heterophil/lymphocyte (H/L) ratio as a criterion for selection for resistance to Salmonella typhimurium in chickens. 2. An infective dose of S. typhimurium was given, directly in the crop, to two groups of chicken selected as Resistant (R) and Sensitive (S) on the bases of H/L ratio. The 99% lower confidence limit was used as a borderline; individuals below the limit were considered R and those above S. Many aspects of immune response were compared, namely: H/L ratio, antibody titre, cellular immunity, phagocytic activity, cortisol concentration, bursa and body weight. 3. The R group exceeded the S in all the studied variables of the immune response, indicating the possibility of using the H/L ratio and its confidence limit to select for general resistance. 4. Due to the within-strain variability in resistance, it seems that immunological and genetic studies should take into consideration separation of individuals into R and S before grouping. Failing to do so might lead to erroneous conclusions as a difference may simply be due to the different numbers of R and S included in each group.

  7. Design and synthesis of pH-sensitive polyamino-ester magneto-dendrimers: Surface functional groups effect on viability of human prostate carcinoma cell lines DU145.

    Science.gov (United States)

    Dayyani, Nahid; Khoee, Sepideh; Ramazani, Ali

    2015-06-15

    Novel pH-sensitive, biocompatible and biodegradable magneto-dendrimers with OH and/or NH2 functional groups based on poly amino-ester were synthesized for delivery of anti-cancer drugs. Magnetite nanoparticles (MNPs) were synthesized by the co-precipitation method and their surfaces were modified by 3-aminopropyl triethoxysilane. The first and second generations of the magneto-dendrimer with hydroxyl end groups were produced by sequential acrylation and Michael addition reactions using the required amounts of acryloyl chloride and diethanolamine, respectively. The dendrimer containing amino functional surface groups up to second generation was synthesized by the same method using the necessary amounts of acryloyl chloride and ethylenediamine. These dendrimers were fully characterized by the Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), dynamic light scattering (DLS) and zeta potential analysis, vibrating-sample magnetometer (VSM), scanning electron microscope (SEM), transmission electron microscopy (TEM) and selected area electron diffraction (SAED). In-vitro release profiles of the drug-loaded magnetic nanoparticles and their cytotoxicity assay were investigated at two pHs (7.4 and 5.8). The hydrolytic degradation behavior of magneto-dendrimers was evaluated in PBS buffer. Our research suggests that magneto-dendrimers having amine or hydroxyl functional groups could be considered as the suitable nanocarriers for therapy applications. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

  8. The translational blocking of α5 and α6 integrin subunits affects migration and invasion, and increases sensitivity to carboplatin of SKOV-3 ovarian cancer cell line.

    Science.gov (United States)

    Villegas-Pineda, Julio César; Toledo-Leyva, Alfredo; Osorio-Trujillo, Juan Carlos; Hernández-Ramírez, Verónica Ivonne; Talamás-Rohana, Patricia

    2017-02-15

    Epithelial ovarian cancer is the most lethal gynecologic malignancy. Integrins, overexpressed in cancer, are involved in various processes that favor the development of the disease. This study focused on determining the degree of involvement of α5, α6 and β3 integrin subunits in the establishment/development of epithelial ovarian cancer (EOC), such as proliferation, migration, invasion, and response to carboplatin. The translation of the α5, α6 and β3 integrins was blocked using morpholines, generating morphant cells for these proteins, which were corroborated by immunofluorescence assays. WST-1 proliferation assay showed that silencing of α5, α6, and β3 integrins does not affect the survival of morphants. Wound healing and transwell chamber assays showed that blocking α5 and α6 integrins decrease, in lesser and greater level respectively, the migratory and the invasive capacity of SKOV-3 cells. Finally, blocking α5 and α6 integrins partially sensitized the cells response to carboplatin, while blocking integrin β3 generated resistance to this drug. Statistical analyses were performed with the GraphPad Prism 5.0 software employing one way and two-way ANOVA tests; data are shown as average±SD. Results suggest that α5 and α6 integrins could become good candidates for chemotherapy targets in EOC. Copyright © 2017 Elsevier Inc. All rights reserved.

  9. Suppression of telomere-binding protein TPP1 resulted in telomere dysfunction and enhanced radiation sensitivity in telomerase-negative osteosarcoma cell line

    Energy Technology Data Exchange (ETDEWEB)

    Qiang, Weiguang [Hubei Cancer Clinical Study Center, Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital, Wuhan University, Wuhan (China); Department of Oncology, The Third Affiliated Hospital, Soochow University, Changzhou (China); Wu, Qinqin [Hubei Cancer Clinical Study Center, Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital, Wuhan University, Wuhan (China); Department of Radiation Oncology, Changzhou Tumor Hospital, Soochow University, Changzhou (China); Zhou, Fuxiang; Xie, Conghua [Hubei Cancer Clinical Study Center, Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital, Wuhan University, Wuhan (China); Wu, Changping, E-mail: wcpzlk@163.com [Department of Oncology, The Third Affiliated Hospital, Soochow University, Changzhou (China); Zhou, Yunfeng, E-mail: yfzhouwhu@163.com [Hubei Cancer Clinical Study Center, Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital, Wuhan University, Wuhan (China)

    2014-03-07

    Highlights: • Down-regulation of TPP1 shortened telomere length in telomerase-negative cells. • Down-regulation of TPP1 induced cell apoptosis in telomerase-negative cells. • Down-regulation of TPP1 increased radiosensitivity in telomerase-negative cells. - Abstract: Mammalian telomeres are protected by the shelterin complex that contains the six core proteins POT1, TPP1, TIN2, TRF1, TRF2 and RAP1. TPP1, formerly known as TINT1, PTOP, and PIP1, is a key factor that regulates telomerase recruitment and activity. In addition to this, TPP1 is required to mediate the shelterin assembly and stabilize telomere. Previous work has found that TPP1 expression was elevated in radioresistant cells and that overexpression of TPP1 led to radioresistance and telomere lengthening in telomerase-positive cells. However, the exact effects and mechanism of TPP1 on radiosensitivity are yet to be precisely defined in the ALT cells. Here we report on the phenotypes of the conditional deletion of TPP1 from the human osteosarcoma U2OS cells using ALT pathway to extend the telomeres.TPP1 deletion resulted in telomere shortening, increased apoptosis and radiation sensitivity enhancement. Together, our findings show that TPP1 plays a vital role in telomere maintenance and protection and establish an intimate relationship between TPP1, telomere and cellular response to ionizing radiation, but likely has the specific mechanism yet to be defined.

  10. The translational blocking of α5 and α6 integrin subunits affects migration and invasion, and increases sensitivity to carboplatin of SKOV-3 ovarian cancer cell line

    Energy Technology Data Exchange (ETDEWEB)

    Villegas-Pineda, Julio César, E-mail: jcvillegas@cinvestav.mx; Toledo-Leyva, Alfredo, E-mail: toledo_leyva@hotmail.com; Osorio-Trujillo, Juan Carlos, E-mail: clostrujillo2@yahoo.com.mx; Hernández-Ramírez, Verónica Ivonne, E-mail: arturomvi@hotmail.com; Talamás-Rohana, Patricia, E-mail: ptr@cinvestav.mx

    2017-02-15

    Epithelial ovarian cancer is the most lethal gynecologic malignancy. Integrins, overexpressed in cancer, are involved in various processes that favor the development of the disease. This study focused on determining the degree of involvement of α5, α6 and β3 integrin subunits in the establishment/development of epithelial ovarian cancer (EOC), such as proliferation, migration, invasion, and response to carboplatin. The translation of the α5, α6 and β3 integrins was blocked using morpholines, generating morphant cells for these proteins, which were corroborated by immunofluorescence assays. WST-1 proliferation assay showed that silencing of α5, α6, and β3 integrins does not affect the survival of morphants. Wound healing and transwell chamber assays showed that blocking α5 and α6 integrins decrease, in lesser and greater level respectively, the migratory and the invasive capacity of SKOV-3 cells. Finally, blocking α5 and α6 integrins partially sensitized the cells response to carboplatin, while blocking integrin β3 generated resistance to this drug. Statistical analyses were performed with the GraphPad Prism 5.0 software employing one way and two-way ANOVA tests; data are shown as average±SD. Results suggest that α5 and α6 integrins could become good candidates for chemotherapy targets in EOC.

  11. Sujiaonori-Derived Algal Biomaterials Inhibit Allergic Reaction in Allergen-Sensitized RBL-2H3 Cell Line and Improve Skin Health in Humans.

    Science.gov (United States)

    Ngatu, Nlandu Roger; Tanaka, Mamoru; Ikeda, Mitsunori; Inoue, Masataka; Kanbara, Sakiko; Nojima, Sayumi

    2017-08-29

    Sujiaonori, a river alga growing in the Kochi prefecture, Japan, contains several bioactive compounds such as sulfated polysaccharides (ulvans), ω-3 fatty acids, and vitamins. Dietary intake of this alga-based supplement has been reported to increase circulatory adiponectin, a salutary hormone that is reported to be associated with healthy longevity and prevents a number of cardiovascular and metabolic disorders. This report highlights the anti-allergic and skin health enhancing effects of Sujiaonori-derived ulvan (Tosalvan) and supplement, respectively. RBL-2H3 cell line was used to investigate the anti-allergic effect of algal SP through the evaluation of β-hexosaminidase activity. Algal sulfated polysaccharides or SP (Tosalvan, Yoshino SP) were extracted from powders of dried alga samples provided by local food manufacturers. Report on the effect of daily dietary intake of Sujiaonori-based supplement on skin health is part of a four-week clinical investigation that, in comparison with a supplement made of 70% corn starch powder and 30% spinach powder mixture (twice 3 g daily), explore the beneficial effects of Sujiaonori algal biomaterial (SBM; 3 g taken twice daily) on cardiovascular, gastrointestinal and skin health in a sample of Japanese women. Transepidermal water loss (TEWL) was the skin health marker used in this study and was measured with the use of a corneometer. Significant reduction of β-hexosaminidase activity was observed in Tosalvan and Yoshino SP-treated cells (vs. control; p allergic potential and that the dietary intake of SBM has a beneficial effect on skin health.

  12. Enhanced benzodiazepine and ethanol actions on cerebellar GABA(A) receptors mediating glutamate release in an alcohol-sensitive rat line.

    Science.gov (United States)

    Schmid, G; Bonanno, G; Raiteri, L; Sarviharju, M; Korpi, E R; Raiteri, M

    1999-09-01

    Granule cell axon terminals of rat cerebellum possess benzodiazepine-insensitive GABA(A) receptors mediating glutamate release. We have investigated the ability of benzodiazepines, ethanol and furosemide to modulate the function of these receptors in the cerebellum of alcohol-tolerant (AT) and alcohol-nontolerant (ANT) rats. AT and ANT synaptosomes, prelabeled with [3H]D-aspartate, were superfused with GABA and various drugs during the K+ -depolarization. GABA similarly enhanced [3H]D-aspartate overflow in AT (EC50 = 1.7 microM) and ANT (EC50 = 3.9 microM) rats in a bicuculline-sensitive manner. Diazepam or zolpidem, at 0.1 microM, potentiated GABA at the GABA(A) receptor of ANT rats, but were ineffective at the AT receptor. Zolpidem acted with great potency (EC50 = 13.6 nM). Ethanol, added at 50 mM, potentiated GABA in ANT rats, but it was inactive at the GABA(A) receptor of the AT cerebellum. Furosemide significantly inhibited the effect of GABA in ANT, but not in AT synaptosomes. Our results show that one GABA(A) receptor (the receptor sited on granule cell terminals which mediates glutamate release) exhibits functional responses to diazepam and ethanol that differ between AT and ANT rats. However, the data with zolpidem and furosemide differ from previous results obtained with membranes of the granule cell layer suggesting that distinct GABA(A) receptor subtypes may exist on axon terminals versus soma/dendrites of granule cells.

  13. Sensitive innovation

    DEFF Research Database (Denmark)

    Søndergaard, Katia Dupret

    Present paper discusses sources of innovation as heterogenic and at times intangible processes. Arguing for heterogeneity and intangibility as sources of innovation originates from a theoretical reading in STS and ANT studies (e.g. Callon 1986, Latour 1996, Mol 2002, Pols 2005) and from field work...... in the area of mental health (Dupret Søndergaard 2009, 2010). The concept of sensitive innovation is developed to capture and conceptualise exactly those heterogenic and intangible processes. Sensitive innovation is therefore primarily a way to understand innovative sources that can be......, but are not necessarily, recognized and acknowledged as such in the outer organisational culture or by management. The added value that qualifies these processes to be defined as “innovative” are thus argued for along different lines than in more traditional innovation studies (e.g. studies that build on the classic...

  14. Line Broadening in White Dwarf Photospheres

    Science.gov (United States)

    Winget, D. E.

    2012-06-01

    White dwarfs are the simplest stars with the simplest surface chemical compositions known. Spectroscopically we detect only hydrogen in surfaces of the vast majority of these stars. The remainders are of various types, including stars with surfaces of nearly pure helium and some apparently massive stars with carbon and oxygen at the photosphere. We will examine the potential offered by the white dwarf stars in the context of both astrophysics and physics. This potential includes studying cosmochronology--establishing the age and evolutionary history of our galaxy and an independent lower limit on the age of the universe, constraining the properties of axions and WIMPS in the context of dark matter models, constraining dark energy by establishing the properties of the massive progenitors of type Ia supernovae, studying nucleosynthesis from their internal composition structure, and crystallization in dense Coulomb plasmas, among many others. Realizing this tremendous scientific potential depends on the determination of two boundary conditions for each star: the surface gravity and effective temperature. To do this, we must establish the photospheric plasma conditions, density and temperature, using observations of the stellar absorption spectra. Our understanding of line broadening appears to be an obstacle, at present. We will discuss the evidence for past theoretical inadequacies in line broadening theory and the hope for recent and future calculations. We will discuss how the experiments underway on the Z-facility at Sandia National Laboratories --where we can create macroscopic uniform plasmas under white dwarf photospheric conditions--will provide the benchmarks for improving our understanding of line broadening under white dwarf photospheric plasma conditions. These experiments will guide future theory and improve our understanding of the white dwarf stars and, through them, the contents and evolution of the cosmos.

  15. Conjugated and Entrapped HPMA-PLA Nano-Polymeric Micelles Based Dual Delivery of First Line Anti TB Drugs: Improved and Safe Drug Delivery against Sensitive and Resistant Mycobacterium Tuberculosis.

    Science.gov (United States)

    Upadhyay, Seema; Khan, Iliyas; Gothwal, Avinash; Pachouri, Praveen K; Bhaskar, N; Gupta, Umesh D; Chauhan, Devendra S; Gupta, Umesh

    2017-09-01

    First line antiTB drugs have several physical and toxic manifestations which limit their applications. RIF is a hydrophobic drug and has low water solubility and INH is hepatotoxic. The main objective of the study was to synthesize, characterize HPMA-PLA co-polymeric micelles for the effective dual delivery of INH and RIF. HPMA-PLA co-polymer and HPMA-PLA-INH (HPI) conjugates were synthesized and characterized by FT-IR and 1H-NMR spectroscopy. Later on RIF loaded HPMA-PLA-INH co-polymeric micelles (PMRI) were formulated and characterized for size, zeta potential and surface morphology (SEM, TEM) as well as critical micellar concentration. The safety was assessed through RBC's interaction study. The prepared PMRI were evaluated through MABA assay against sensitive and resistant strains of M. Tuberculosis. Size, zeta and entrapment efficiency for RIF loaded HPMA-PLA-INH polymeric micelles (PMRI) was 87.64 ± 1.98 nm, -19 ± 1.93 mV and 97.2 ± 1.56%, respectively. In vitro release followed controlled and sustained delivery pattern. Sustained release was also supported by release kinetics. Haemolytic toxicity of HPI and PMRI was 8.57 and 7.05% (p HPMA-PLA polymeric micelles (PMRI) were more effective against sensitive and resistant M tuberculosis. The developed approach can lead to improved patient compliance and reduced dosing in future, offering improved treatment of tuberculosis.

  16. High Line

    DEFF Research Database (Denmark)

    Kiib, Hans

    2015-01-01

    factories and warehouses on Gansevoort Street. Today the High Line is a beautiful park covered with new tiles, viewing platforms and smaller recreational areas. The park bridge has simple, uniform, urban fittings and features a variety of flowering plants, grasses, shrubs and trees from around the world......, and galleries. With the High Line, a new urban architectural typology has been created that is aesthetically enriching and sets new standards for urban transformation and urban life. “The park accommodates the wild, the cultivated, the intimate and the social. Access points are durational experiences designed...

  17. Sensitivity analysis

    Science.gov (United States)

    ... page: //medlineplus.gov/ency/article/003741.htm Sensitivity analysis To use the sharing features on this page, please enable JavaScript. Sensitivity analysis determines the effectiveness of antibiotics against microorganisms (germs) ...

  18. production lines

    Directory of Open Access Journals (Sweden)

    Jingshan Li

    2000-01-01

    Full Text Available In this work, serial production lines with finished goods buffers operating in the pull regime are considered. The machines are assumed to obey Bernoulli reliability model. The problem of satisfying customers demand is addressed. The level of demand satisfaction is quantified by the due-time performance (DTP, which is defined as the probability to ship to the customer a required number of parts during a fixed time interval. Within this scenario, the definitions of DTP bottlenecks are introduced and a method for their identification is developed.

  19. Parallel Lines

    Directory of Open Access Journals (Sweden)

    James G. Worner

    2017-05-01

    Full Text Available James Worner is an Australian-based writer and scholar currently pursuing a PhD at the University of Technology Sydney. His research seeks to expose masculinities lost in the shadow of Australia’s Anzac hegemony while exploring new opportunities for contemporary historiography. He is the recipient of the Doctoral Scholarship in Historical Consciousness at the university’s Australian Centre of Public History and will be hosted by the University of Bologna during 2017 on a doctoral research writing scholarship.   ‘Parallel Lines’ is one of a collection of stories, The Shapes of Us, exploring liminal spaces of modern life: class, gender, sexuality, race, religion and education. It looks at lives, like lines, that do not meet but which travel in proximity, simultaneously attracted and repelled. James’ short stories have been published in various journals and anthologies.

  20. Real-time in vivo monitoring of circadian E-box enhancer activity: a robust and sensitive zebrafish reporter line for developmental, chemical and neural biology of the circadian clock.

    Science.gov (United States)

    Weger, Meltem; Weger, Benjamin D; Diotel, Nicolas; Rastegar, Sepand; Hirota, Tsuyoshi; Kay, Steve A; Strähle, Uwe; Dickmeis, Thomas

    2013-08-15

    The circadian clock co-ordinates physiology and behavior with the day/night cycle. It consists of a transcriptional-translational feedback loop that generates self-sustained oscillations in transcriptional activity with a roughly 24h period via E-box enhancer elements. Numerous in vivo aspects of core clock feedback loop function are still incompletely understood, including its maturation during development, tissue-specific activity and perturbation in disease states. Zebrafish are promising models for biomedical research due to their high regenerative capacity and suitability for in vivo drug screens, and transgenic zebrafish lines are valuable tools to study transcriptional activity in vivo during development. To monitor the activity of the core clock feedback loop in vivo, we created a transgenic zebrafish line expressing a luciferase reporter gene under the regulation of a minimal promoter and four E-boxes. This Tg(4xE-box:Luc) line shows robust oscillating reporter gene expression both under light-dark cycles and upon release into constant darkness. Luciferase activity starts to oscillate during the first days of development, indicating that the core clock loop is already functional at an early stage. To test whether the Tg(4xE-box:Luc) line could be used in drug screens aimed at identifying compounds that target the circadian clock in vivo, we examined drug effects on circadian period. We were readily able to detect period changes as low as 0.7h upon treatment with the period-lengthening drugs lithium chloride and longdaysin in an assay set-up suitable for large-scale screens. Reporter gene mRNA expression is also detected in the adult brain and reveals differential clock activity across the brain, overlapping with endogenous clock gene expression. Notably, core clock activity is strongly correlated with brain regions where neurogenesis takes place and can be detected in several types of neural progenitors. Our results demonstrate that the Tg(4x

  1. VT Digital Line Graph Miscellaneous Transmission Lines

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) This datalayer is comprised of Miscellaineous Transmission Lines. Digital line graph (DLG) data are digital representations of cartographic...

  2. Climate Sensitivity

    Energy Technology Data Exchange (ETDEWEB)

    Lindzen, Richard [M.I.T.

    2011-11-09

    Warming observed thus far is entirely consistent with low climate sensitivity. However, the result is ambiguous because the sources of climate change are numerous and poorly specified. Model predictions of substantial warming aredependent on positive feedbacks associated with upper level water vapor and clouds, but models are notably inadequate in dealing with clouds and the impacts of clouds and water vapor are intimately intertwined. Various approaches to measuring sensitivity based on the physics of the feedbacks will be described. The results thus far point to negative feedbacks. Problems with these approaches as well as problems with the concept of climate sensitivity will be described.

  3. A multicenter, non-randomized, phase II study of docetaxel and carboplatin administered every 3 weeks as second line chemotherapy in patients with first relapse of platinum sensitive epithelial ovarian, peritoneal or fallopian tube cancer

    DEFF Research Database (Denmark)

    Wang, Yun; Herrstedt, Jørn; Havsteen, Hanne

    2014-01-01

    of 398 cycles were given. Grade 3/4 neutropenia was seen in 80% (59 of 74) patients with an incidence of febrile neutropenia of 16%. Grade 2/3 sensory peripheral neuropathy occurred in 7% of patients, but no grade 4 sensory peripheral neuropathy was observed. Sixty patients were evaluable for response...... of platinum-sensitive ovarian, peritoneal and Fallopian tube cancer. The major toxicity was neutropenia, while the frequency of peripheral neuropathy was low....

  4. Gluten Sensitivity

    Science.gov (United States)

    Gluten is a protein found in wheat, rye, and barley. It is found mainly in foods but ... products like medicines, vitamins, and supplements. People with gluten sensitivity have problems with gluten. It is different ...

  5. In chronic myeloid leukemia patients on second-line tyrosine kinase inhibitor therapy, deep sequencing of BCR-ABL1 at the time of warning may allow sensitive detection of emerging drug-resistant mutants.

    Science.gov (United States)

    Soverini, Simona; De Benedittis, Caterina; Castagnetti, Fausto; Gugliotta, Gabriele; Mancini, Manuela; Bavaro, Luana; Machova Polakova, Katerina; Linhartova, Jana; Iurlo, Alessandra; Russo, Domenico; Pane, Fabrizio; Saglio, Giuseppe; Rosti, Gianantonio; Cavo, Michele; Baccarani, Michele; Martinelli, Giovanni

    2016-08-02

    Imatinib-resistant chronic myeloid leukemia (CML) patients receiving second-line tyrosine kinase inhibitor (TKI) therapy with dasatinib or nilotinib have a higher risk of disease relapse and progression and not infrequently BCR-ABL1 kinase domain (KD) mutations are implicated in therapeutic failure. In this setting, earlier detection of emerging BCR-ABL1 KD mutations would offer greater chances of efficacy for subsequent salvage therapy and limit the biological consequences of full BCR-ABL1 kinase reactivation. Taking advantage of an already set up and validated next-generation deep amplicon sequencing (DS) assay, we aimed to assess whether DS may allow a larger window of detection of emerging BCR-ABL1 KD mutants predicting for an impending relapse. a total of 125 longitudinal samples from 51 CML patients who had acquired dasatinib- or nilotinib-resistant mutations during second-line therapy were analyzed by DS from the time of failure and mutation detection by conventional sequencing backwards. BCR-ABL1/ABL1%(IS) transcript levels were used to define whether the patient had 'optimal response', 'warning' or 'failure' at the time of first mutation detection by DS. DS was able to backtrack dasatinib- or nilotinib-resistant mutations to the previous sample(s) in 23/51 (45 %) pts. Median mutation burden at the time of first detection by DS was 5.5 % (range, 1.5-17.5 %); median interval between detection by DS and detection by conventional sequencing was 3 months (range, 1-9 months). In 5 cases, the mutations were detectable at baseline. In the remaining cases, response level at the time mutations were first detected by DS could be defined as 'Warning' (according to the 2013 ELN definitions of response to 2nd-line therapy) in 13 cases, as 'Optimal response' in one case, as 'Failure' in 4 cases. No dasatinib- or nilotinib-resistant mutations were detected by DS in 15 randomly selected patients with 'warning' at various timepoints, that later turned into optimal

  6. Western Alaska ESI: FISHL (Fish Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for anadromous fish species in Western Alaska. Vector lines in this data set represent species occurrences...

  7. Louisiana ESI: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for coastal...

  8. Maryland ESI: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for Maryland. The...

  9. Alabama ESI: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for Alabama. The...

  10. Virginia ESI: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for Virginia. The...

  11. North Slope, Alaska ESI: FISHL (Fish Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for anadromous and freshwater fish species for the North Slope of Alaska. Vector lines in this data set...

  12. Program of telluric lines monitoring

    Directory of Open Access Journals (Sweden)

    Vince I.

    2006-01-01

    Full Text Available A new observational program of telluric lines monitoring was introduced at Belgrade Astronomical Observatory. The ultimate goal of this program is to investigate the properties of Earth’s atmosphere through modeling the observed profiles of telluric lines. The program is intend to observe infrared molecular oxygen lines that were selected according to spectral sensitivity of the available CCD camera. In this paper we give the initial and the final selection criteria for spectral lines included in the program the description of equipment and procedures used for observations and reduction, a review of preliminary observational results with the estimated precision, and a short discussion on the comparison of the theoretical predictions and the measurements.

  13. Multiple Lines of Evidence

    Energy Technology Data Exchange (ETDEWEB)

    Amidan, Brett G. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Venzin, Alexander M. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Bramer, Lisa M. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2015-06-03

    This paper discusses the process of identifying factors that influence the contamination level of a given decision area and then determining the likelihood that the area remains unacceptable. This process is referred to as lines of evidence. These lines of evidence then serve as inputs for the stratified compliance sampling (SCS) method, which requires a decision area to be divided into strata based upon contamination expectations. This is done in order to focus sampling efforts more within stratum where contamination is more likely and to use the domain knowledge about these likelihoods of the stratum remaining unacceptable to buy down the number of samples necessary, if possible. Two different building scenarios were considered as an example (see Table 3.1). SME expertise was elicited concerning four lines of evidence factors (see Table 3.2): 1) amount of contamination that was seen before decontamination, 2) post-decontamination air sampling information, 3) the applied decontaminant information, and 4) the surface material. Statistical experimental design and logistic regression modelling were used to help determine the likelihood that example stratum remained unacceptable for a given example scenario. The number of samples necessary for clearance was calculated by applying the SCS method to the example scenario, using the estimated likelihood of each stratum remaining unacceptable as was determined using the lines of evidence approach. The commonly used simple random sampling (SRS) method was also used to calculate the number of samples necessary for clearance for comparison purposes. The lines of evidence with SCS approach resulted in a 19% to 43% reduction in total number of samples necessary for clearance (see Table 3.6). The reduction depended upon the building scenario, as well as the level of percent clean criteria. A sensitivity analysis was also performed showing how changing the estimated likelihoods of stratum remaining unacceptable affect the number

  14. A phase 2 evaluation of irofulven as second-line treatment of recurrent or persistent intermediately platinum-sensitive ovarian or primary peritoneal cancer: a Gynecologic Oncology Group trial.

    Science.gov (United States)

    Schilder, Russell J; Blessing, John A; Shahin, Mark S; Miller, David S; Tewari, Krishnansu Sujata; Muller, Carolyn Y; Warshal, David P; McMeekin, Scott; Rotmensch, Jacob

    2010-10-01

    This multicenter phase 2 trial was conducted by the Gynecologic Oncology Group to evaluate the activity and the safety of irofulven in patients with recurrent epithelial ovarian cancer. Eligible patients had documented recurrent ovarian cancer 6 to 12 months after receiving a front-line platinum-based regimen and no other chemotherapy. Patients were required to have measurable disease, performance status of 0 to 2, and adequate bone marrow, hepatic, and renal functions before study entry. The dose of irofulven was 0.45 mg/kg intravenously on days 1 and 8 every 21 days. Responses were defined by Response Evaluation Criteria in Solid Tumors. Fifty-five of 61 enrolled patients were evaluable for response and toxicity. There were 7 partial responses (12.7%), and 30 patients (54.6%) had stable disease. Median progression-free and overall survival were 6.4 months (1.3-37.5 months) and 22.1 months or more (2.8-57.8+ months), respectively. Patients received a median of 3 cycles (range, 1-21) of protocol therapy. Grade 4 hematologic toxicity was limited to reversible neutropenia and thrombocytopenia. Grade 4 nonhematologic toxicity was limited to one patient with anorexia and another with hypomagnesemia. Irofulven administered at this dose and schedule was well tolerated but had modest activity as a single agent.

  15. A phase II evaluation of Irofulven (IND#55804, NSC#683863) as second-line treatment of recurrent or persistent intermediately platinum-sensitive ovarian or primary peritoneal cancer: A Gynecologic Oncology Group trial

    Science.gov (United States)

    Schilder, Russell J.; Blessing, John A.; Shahin, Mark S.; Miller, David S; Tewari, Krishnansu Sujata; Muller, Carolyn Y.; Warshal, David; McMeekin, D. Scott; Rotmensch, Jacob

    2010-01-01

    This multi-center phase II trial was conducted by the Gynecologic Oncology Group to evaluate the activity and safety of irofulven in patients with recurrent epithelial ovarian cancer. Eligible patients had documented recurrent ovarian cancer 6-12 months after receiving a front-line platinum-based regimen and no other chemotherapy. Patients were required to have measurable disease, performance status of 0-2, and adequate bone marrow, hepatic, and renal function prior to study entry. The dose of irofulven was 0.45 mg/kg IV on days 1 and 8 every 21 days. Responses were defined by RECIST. Fifty-five of 61 enrolled patients were evaluable for response and toxicity. There were seven partial responses (12.7%) and 30 patients (54.6%) had stable disease. Median progression-free and overall survival were 6.4 mo (1.3-37.5) and 22.1+ mo (2.8-57.8+), respectively. Patients received a median of three cycles (range 1-21) of protocol therapy. Grade 4 hematologic toxicity was limited to reversible neutropenia and thrombocytopenia. Grade 4 non-hematologic toxicity was limited to one patient with anorexia and another with hypomagnesemia. Irofulven administered at this dose and schedule was well tolerated but had modest activity as a single agent. PMID:21495215

  16. Development of a Handheld Line Information Reader and Generator for Efficient Management of Optical Communication Lines.

    Science.gov (United States)

    Lee, Jaeyul; Kwon, Hyungwoo; Song, Jaewon; Jeon, Mansik; Kim, Jeehyun

    2017-08-24

    A handheld line information reader and a line information generator were developed for the efficient management of optical communication lines. The line information reader consists of a photo diode, trans-impedance amplifier, voltage amplifier, microcontroller unit, display panel, and communication modules. The line information generator consists of a laser diode, laser driving circuits, microcontroller unit, and communication modules. The line information reader can detect the optical radiation field of the test line by bending the optical fiber. To enhance the sensitivity of the line information reader, an additional lens was used with a focal length of 4.51 mm. Moreover, the simulation results obtained through BeamPROP® software from Synopsys, Inc. demonstrated a stronger optical radiation field of the fiber due to a longer transmission wavelength and larger bending angle of the fiber. Therefore, the developed devices can be considered as useful tools for the efficient management of optical communication lines.

  17. Multiple Chemical Sensitivity

    DEFF Research Database (Denmark)

    Dantoft, Thomas Meinertz

    Multiple chemical sensitivity (MCS) is a chronic disorder characterized by reports of symptoms from various organ systems attributed by the individuals to exposure to common odors and airborne chemicals in doses far below those known to induce toxic effects. There exists a general lack of knowledge...... controls at baseline, immediately after and four hours after a controlled chemical (n-butanol) exposure, in an exposure chamber previously verified to induce symptom elicitation in MCS subjects. In manuscript III, mucosal lining fluid samples were collected and levels of 19 cytokines and chemokines were...

  18. Stability of the LHC transfer lines

    CERN Document Server

    Kain, V; Bartmann, W; Bracco, C; Goddard, B; Meddahi, M; Uythoven, J; Wenninger, J

    2011-01-01

    The LHC is filled from the SPS through two 3 km transfer lines. The injected beam parameters need to be well under control for luminosity performance, machine protection and operational efficiency. Small fractions of beam loss on the transfer line collimation system create showers which can trigger the sensitive LHC beam loss monitor system nearby and cause a beam abort during filling. The stability of the transfer line trajectory through the collimators is particularly critical in this respect. This paper will report on the transfer line trajectory stability during the proton run in 2011, correlations with injection losses, correction frequency and the most likely sources for the observed oscillations.

  19. Peripheral intravenous line - infants

    Science.gov (United States)

    PIV - infants; Peripheral IV - infants; Peripheral line - infants; Peripheral line - neonatal ... A peripheral intravenous line (PIV) is a small, short, plastic tube, called a catheter. A health care provider puts the PIV through the ...

  20. Sewer Main Pipe Lines

    Data.gov (United States)

    U.S. Environmental Protection Agency — This is a line feature dataset with lines across the island of Guam. These lines represent the sewer mains that carry refuse to the sewer plants for processing and...

  1. Sensitivity, Recalculated

    Energy Technology Data Exchange (ETDEWEB)

    Brodsky, J. P. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2016-11-07

    Prior to this study, the sensitivity in nEXO was calculated using an approximate method that does not provide 90% coverage. I find it useful to understand the confidence level of a limit by focusing on the confidence level as a property of the method used to find the limit.A particular method (algorithm,formula) is applied to an experiment to produce a limit. That experiment was conducted in a particular universe with certain values of the physical parameters. We’d like a method than produces a correct limit for 90% of the experiments that could run. We also want that to be true for every universe—that is, regardless of the physical parameter value, the method should still produce a correct limit 90% of the time.

  2. Abstract Line Designs

    Science.gov (United States)

    Nevinskas, Nancy

    2011-01-01

    In this article, the author describes a unit on the exploration of line. The unit was composed of two individual line lessons. In the first lesson, students were introduced to line as an element of design. They were asked to describe different types of lines, and look for them in art reproductions. The second lesson in the unit involved painting…

  3. WIMP Dark Matter interpretation of Higgs results

    CERN Document Server

    Wang, Renjie; The ATLAS collaboration

    2017-01-01

    The results from searching for dark matter either directly from invisible decay of Higgs bosons or in association with a Higgs boson at the LHC are presented. No significant excess is found beyond the Standard Model prediction, and upper limits are set on the production cross section times branching fraction using data collected in proton-proton collisions at center-of-mass energies of 13 TeV by the ATLAS and CMS detectors. An interpreted upper limit is presented on the allowed dark matter-nucleon scattering cross section.

  4. Community LINE Source Model (C-LINE)

    Science.gov (United States)

    This presentation provides an introduction for the live demo and explains the purpose of C-LINE and its key features. C-LINE is a web-based model designed to inform the community user of local air quality impacts due to mobile-sources in their region of interest using a simplifie...

  5. Detecção da citotoxicidade de materiais biocompatíveis nas linhagens celulares MRC-5, HeLa e RC-IAL MRC-5, HeLa and RC-IAL cell lines sensitivity for detection of cytotoxicity of biocompatible materials

    Directory of Open Access Journals (Sweden)

    Aurea S. Cruz

    1992-04-01

    Full Text Available A sensibilidade de uma linhagem celular diplóide e duas heteroplóides, para a detecção de citotoxicidade através do método de difusão em camada de ágar sobre culturas celulares, foi avaliada experimentalmente com solução de ácido ascórbico em diferentes concentrações e, na prática, frente a 562 amostras de 21 diferentes materiais industriais enviados para análise na Seção de Culturas Celulares do Instituto Adolfo Lutz. A linhagem celular heteroplóide designada RC-IAL apresentou, em relação às linhagens MRC-5 e HeLa, maior sensibilidade porque revelou a presença de efeito citotóxico nas menores concentrações utilizadas (10 e 25 ug/ml do ácido ascórbico e apresentou maior diâmetro do halo citotóxico em 15 amostras e igual diâmetro em 16 das 43 amostras (7,6% que resultaram positivas. Nas 43 amostras positivas, a linhagem MRC-5 não revelou citotoxicidade em 3 amostras de espuma e 1 de resina acrílica. O polivinilcloreto (PVC e o polietileno, raramente revelaram positividade, enquanto plástico, algodão e resinas acrílicas revelaram citotoxicidade ao redor de 5%. Em vista dos resultados é discutida a proposta da utilização da linhagem RC-IAL e HeLa para a continuidade das futuras análises solicitadas ao Instituto Adolfo LutzThe sensitivity of diploid and heteroploid cell lines for detection of cytotoxicity using the agar diffusion method on cell culture, was tested with ascorbic acid solution of different concentrations. A total of 562 samples of 21 various materials were tested. The heteroploid cell line, RC-IAL, showed in relation to the MRC-5 and HeLa cell lines, greater sensitivity because it showed the presence of cytotoxic effect with the lowest concentration used (10 and 25ug/ml of ascorbic acid and showed greater diameter of cytotoxic halo in 15 samples and equal diameter in 16 of the 43 positive samples (7.6%. Out of 43 positive samples, the MRC-5 line did not show cytotoxicity in 3 sponge samples and

  6. Resolution, LER, and sensitivity limitations of photoresists

    Science.gov (United States)

    Gallatin, Gregg M.; Naulleau, Patrick; Niakoula, Dimitra; Brainard, Robert; Hassanein, Elsayed; Matyi, Richard; Thackeray, Jim; Spear, Kathleen; Dean, Kim

    2008-03-01

    Recent experimental results and modeling both indicate that whereas it is possible to optimize a photoresist and process to achieve separately a desired resolution or line edge roughness or sensitivity, it will be difficult if not impossible to achieve all three simultaneously using current standard chemically amplified photoresists and processes. This tradeoff among Resolution, Line Edge Roughness (LER) and Sensitivity is termed the RLS tradeoff. Here we review the progress to date of a SEMATECH-funded program to develop an experimentally verified model of the relationship among resolution, LER and sensitivity and use it to determine approaches for "breaking" the RLS tradeoff.

  7. Nuclear reactor overflow line

    Science.gov (United States)

    Severson, Wayne J.

    1976-01-01

    The overflow line for the reactor vessel of a liquid-metal-cooled nuclear reactor includes means for establishing and maintaining a continuous bleed flow of coolant amounting to 5 to 10% of the total coolant flow through the overflow line to prevent thermal shock to the overflow line when the reactor is restarted following a trip. Preferably a tube is disposed concentrically just inside the overflow line extending from a point just inside the reactor vessel to an overflow tank and a suction line is provided opening into the body of liquid metal in the reactor vessel and into the annulus between the overflow line and the inner tube.

  8. Difference in membrane repair capacity between cancer cell lines and a normal cell line

    DEFF Research Database (Denmark)

    Frandsen, Stine Krog; McNeil, Anna K.; Novak, Ivana

    2016-01-01

    Electroporation-based treatments and other therapies that permeabilize the plasma membrane have been shown to be more devastating to malignant cells than to normal cells. In this study, we asked if a difference in repair capacity could explain this observed difference in sensitivity. Membrane...... repair was investigated by disrupting the plasma membrane using laser followed by monitoring fluorescent dye entry over time in seven cancer cell lines, an immortalized cell line, and a normal primary cell line. The kinetics of repair in living cells can be directly recorded using this technique......, providing a sensitive index of repair capacity. The normal primary cell line of all tested cell lines exhibited the slowest rate of dye entry after laser disruption and lowest level of dye uptake. Significantly, more rapid dye uptake and a higher total level of dye uptake occurred in six of the seven tested...

  9. In-line phase sensitive amplifier based on PPLN waveguides

    National Research Council Canada - National Science Library

    Umeki, Takeshi; Asobe, Masaki; Takenouchi, Hirokazu

    2013-01-01

    ... was achieved using degenerate parametric amplification in a periodically poled LiNbO(3) (PPLN) waveguide. The phase regeneration effect was examined for a degraded signal by means of constellation analyses and bit-error rate measurements...

  10. First on-line results from the CRIS (Collinear Resonant Ionisation Spectroscopy) beam line at ISOLDE

    Energy Technology Data Exchange (ETDEWEB)

    Procter, T. J., E-mail: thomas.procter@postgrad.manchester.ac.uk; Flanagan, K. T. [University of Manchester (United Kingdom); Collaboration: CRIS Collaboration

    2013-04-15

    The CRIS (Collinear Resonant Ionisation Spectroscopy) experiment at the on-line isotope separator facility, ISOLDE, CERN, has been constructed for high-sensitivity laser spectroscopy measurements on radioactive isotopes. The technique determines the magnetic dipole and electric quadrupole moments, nuclear spin and changes in mean-square charge radii of exotic nuclei via measurement of their hyperfine structures and isotope shifts. In November 2011 the first on-line run was performed using the CRIS beam line, when the hyperfine structure of {sup 207}Fr was successfully measured. This paper will describe the technique and experimental setup of CRIS and present the results from the first on-line experiment.

  11. VT Electric Transmission Line Corridors - corridor lines

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) The ELTRN layer depicts electric transmission line corridors in Vermont. Various methods have been used to digitize features. The data layer...

  12. Peripheral arterial line (image)

    Science.gov (United States)

    A peripheral arterial line is a small, short plastic catheter placed through the skin into an artery of the arm or leg. The purpose of a peripheral arterial line is to allow continuous monitoring of blood pressure ...

  13. Homotopic Polygonal Line Simplification

    DEFF Research Database (Denmark)

    Deleuran, Lasse Kosetski

    This thesis presents three contributions to the area of polygonal line simplification, or simply line simplification. A polygonal path, or simply a path is a list of points with line segments between the points. A path can be simplified by morphing it in order to minimize some objective function...

  14. Series Transmission Line Transformer

    Science.gov (United States)

    Buckles, Robert A.; Booth, Rex; Yen, Boris T.

    2004-06-29

    A series transmission line transformer is set forth which includes two or more of impedance matched sets of at least two transmissions lines such as shielded cables, connected in parallel at one end ans series at the other in a cascading fashion. The cables are wound about a magnetic core. The series transmission line transformer (STLT) which can provide for higher impedance ratios and bandwidths, which is scalable, and which is of simpler design and construction.

  15. Southeast Alaska ESI: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for Southeast...

  16. Alabama ESI: ESI (Shoreline Types - Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing the shoreline and coastal habitats for Alabama, classified according to the Environmental Sensitivity...

  17. American Samoa ESI: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for American...

  18. North Slope, Alaska ESI: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for the North...

  19. Columbia River ESI: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for Columbia...

  20. Coastal Resources Atlas: Long Island: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for Long Island,...

  1. Bristol Bay, Alaska Subarea ESI: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for the Bristol...

  2. Western Alaska ESI: STREAMS (River and Stream Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines representing inland linear hydrography features used in the creation of the Environmental Sensitivity Index (ESI) for Western...

  3. Silencing of Taxol-Sensitizer Genes in Cancer Cells: Lack of Sensitization Effects

    Energy Technology Data Exchange (ETDEWEB)

    Huang, Shang-Lang [Department of Biochemistry and Molecular Biology, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan (China); Chao, Chuck C.-K., E-mail: cckchao@mail.cgu.edu.tw [Department of Biochemistry and Molecular Biology, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan (China); Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan (China); Department of Medical Research and Development, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan (China)

    2015-06-16

    A previous genome-wide screening analysis identified a panel of genes that sensitize the human non-small-cell lung carcinoma cell line NCI-H1155 to taxol. However, whether the identified genes sensitize other cancer cells to taxol has not been examined. Here, we silenced the taxol-sensitizer genes identified (acrbp, atp6v0d2, fgd4, hs6st2, psma6, and tubgcp2) in nine other cancer cell types (including lung, cervical, ovarian, and hepatocellular carcinoma cell lines) that showed reduced cell viability in the presence of a sub-lethal concentration of taxol. Surprisingly, none of the genes studied increased sensitivity to taxol in the tested panel of cell lines. As observed in H1155 cells, SKOV3 cells displayed induction of five of the six genes studied in response to a cell killing dose of taxol. The other cell types were much less responsive to taxol. Notably, four of the five inducible taxol-sensitizer genes tested (acrbp, atp6v0d2, psma6, and tubgcp2) were upregulated in a taxol-resistant ovarian cancer cell line. These results indicate that the previously identified taxol-sensitizer loci are not conserved genetic targets involved in inhibiting cell proliferation in response to taxol. Our findings also suggest that regulation of taxol-sensitizer genes by taxol may be critical for acquired cell resistance to the drug.

  4. Minnesota County Boundaries - lines

    Data.gov (United States)

    Minnesota Department of Natural Resources — Minnesota county boundaries derived from a combination of 1:24,000 scale PLS lines, 1:100,000 scale TIGER, 1:100,000 scale DLG, and 1:24,000 scale hydrography lines....

  5. Database of emission lines

    Science.gov (United States)

    Binette, L.; Ortiz, P.; Joguet, B.; Rola, C.

    1998-11-01

    A widely accessible data bank (available through Netscape) and consiting of all (or most) of the emission lines reported in the litterature is being built. It will comprise objects as diverse as HII regions, PN, AGN, HHO. One of its use will be to define/refine existing diagnostic emission line diagrams.

  6. New approaches in sensitive chiral CE.

    Science.gov (United States)

    Sánchez-Hernández, Laura; Guijarro-Diez, Miguel; Marina, María Luisa; Crego, Antonio L

    2014-01-01

    CE has shown to have a big potential for chiral separations, with advantages such as high efficiency, high resolution, and low sample and reagents consumption. Nevertheless, when UV detection is employed, CE has some drawbacks, especially the low sensitivity obtained due to the short optical path length. Notwithstanding, sensitivity improvements can be achieved when different approaches are employed, such as sample treatment strategies (off-line or on-line), in-capillary sample preconcentration techniques, and/or alternative detection systems to UV-Vis (such as fluorescence, conductimetry, electrochemiluminiscence, MS, etc.). This article reviews the most recent methodological and instrumental advances reported from June 2011 to May 2013 for enhancing the sensitivity in chiral analysis by CE. The sensitivity achieved for the enantioseparated analytes and the applications carried out using the developed methodologies are also summarized. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  7. Susceptibility testing of fish cell lines for virus isolation

    DEFF Research Database (Denmark)

    Ariel, Ellen; Skall, Helle Frank; Olesen, Niels Jørgen

    2009-01-01

    compare susceptibility between cell lines and between lineages within a laboratory and between laboratories (Inter-laboratory Proficiency Test). The objective being that the most sensitive cell line and lineages are routinely selected for diagnostic purposes.In comparing cell lines, we simulated "non......-cell-culture-adapted" virus by propagating the virus in heterologous cell lines to the one tested. A stock of test virus was produced and stored at - 80 °C and tests were conducted biannually. This procedure becomes complicated when several cell lines are in use and does not account for variation among lineages. In comparing...... cell lineages, we increased the number of isolates of each virus, propagated stocks in a given cell line and tested all lineages of that line in use in the laboratory. Testing of relative cell line susceptibility between laboratories is carried out annually via the Inter-laboratory Proficiency Test...

  8. Comprehensive pharmacological profiling of neurofibromatosis cell lines.

    Science.gov (United States)

    Guo, Jianman; Grovola, Michael R; Xie, Hong; Coggins, Grace E; Duggan, Patrick; Hasan, Rukhsana; Huang, Jiale; Lin, Danny W; Song, Claire; Witek, Gabriela M; Berritt, Simon; Schultz, David C; Field, Jeffrey

    2017-01-01

    Patients with Neurofibromatosis type 1 (NF1) and Neurofibromatosis type 2 (NF2) are predisposed to tumors of the nervous system. NF1 patients predominantly develop neurofibromas, and Malignant Peripheral Nerve Sheath Tumors (MPNST) while NF2 patients develop schwannomas and meningiomas. Here we quantified the drug sensitivities of NF1 and NF2 tumor cell lines in a high throughput platform. The platform contained a comprehensive collection of inhibitors of MEK, RAF, RAS, farnesyl transferase, PAK and ERK, representative drugs against many other cancer pathways including Wnt, Hedgehog, p53, EGF, HDAC, as well as classical cytotoxic agents recommended for treating MPNST, such as doxorubicin and etoposide. We profiled seven NF1-associated MPNST cell lines (ST88-14, ST88-3, 90-8, sNF02.2, T265, S462TY, SNF96.2), one sporadic MPNST cell line (STS26), one schwannoma from a NF2 patient (HEI193), one NF2-deficient malignant meningioma (KT21-MG-Luc5D), one mouse NF2 schwannoma (SC4) and one sporadic rat schwannoma (RT4-67 or RT4). NF1 cells were primarily distinguished from NF2 cells and the sporadic MPNST cell line by their sensitivity to MEK and ERK inhibitors, and to a smaller extent their sensitivity to BH3 mimetics and farnesyl transferase inhibitors. The platform was highly successful in predicting the effects of clinical trials for Neurofibromas.

  9. Temperature measurements via narrow line laser absorption of carbon dioxide

    Energy Technology Data Exchange (ETDEWEB)

    Wooldridge, M.S. [Texas A and M Univ., College Station, TX (United States). Dept. of Mechanical Engineering

    1996-12-31

    Theoretical development for temperature measurements via narrow line, infrared absorption of carbon dioxide (CO{sub 2}) is presented. The proposed technique is based on rapid-scanning of two adjacent absorption line shapes. Spectroscopic considerations for sensitivity to temperature measurements are discussed. Several line pairs are evaluated, and the R(58) and R(60) transitions of the (00{sup 0}1){l_arrow}(00{sup 0}0) band are suggested for use in high temperature measurements for combustion systems.

  10. Glue-paste linings

    DEFF Research Database (Denmark)

    Fuster-López, Laura; Andersen, Cecil Krarup; Bouillon, Nicolas

    2017-01-01

    Glue-paste linings of (Western) canvas paintings have been performed with a variety of materials throughout history and are present in a very significant amount of artworks in collections in Europe and elsewhere. Cereal flours and animal glues were usually the main ingredients because they were...... processes of lined canvas paintings. The impact of cyclic relative humidity on the biological and physical stability of the laminate structure is reported. Carefully constructed mock-ups, simulating lined canvas paintings, were used to examine the effect of pest infestation, mould growth, mechanical damage...

  11. Transnationaliseringen af Maersk Line

    DEFF Research Database (Denmark)

    Sornn-Friese, Henrik

    2017-01-01

    Maersk Line er verdens førende containerrederi og blandt de mest betydningsfulde virksomheder i Danmark. Den globale førerposition blev opnået på relativt kort tid og var et resultat af rederiets beslutning i 1973 om at gå helhjertet ind i containerskibsfarten. Beslutningen blev startskuddet til...... Maersk Lines dybe internationalisering, hvor rederiet ændrede sig fra at være en overvejende danske virksomhed, der betjente internationale markeder, til at være en genuin transnational virksomhed. Med fokus på opbygningen af Maersk Lines globale organisation og særligt etableringen af egne kontorer i...

  12. Wood pole overhead lines

    CERN Document Server

    Wareing, Brian

    2005-01-01

    This new book concentrates on the mechanical aspects of distribution wood pole lines, including live line working, environmental influences, climate change and international standards. Other topics include statutory requirements, safety, profiling, traditional and probabilistic design, weather loads, bare and covered conductors, different types of overhead systems, conductor choice, construction and maintenance. A section has also been devoted to the topic of lightning, which is one of the major sources of faults on overhead lines. The book focuses on the effects of this problem and the strate

  13. Veterans Crisis Line

    Data.gov (United States)

    Department of Veterans Affairs — The caring responders at the Veterans Crisis Line are specially trained and experienced in helping Veterans of all ages and circumstances. Some of the responders are...

  14. MODIFIED METHOD OF LINES

    Directory of Open Access Journals (Sweden)

    Stankevich Anatoliy Nikolaevich

    2016-08-01

    Full Text Available The main idea of the method of solving multidimensional boundary problems is reduction of initial differential equations in partial derivatives to the system of ordinary differential equations. One-dimensional resolving equations allow extending the potential of the method of lines. Although efficient and highly-precise numerical methods have been developed for solution of one-dimensional and initial-boundary value problems their use is impossible in the method of lines. The author considers a new method of lines which is used in order to reduce the dimensionality of multidimensional problems of structural mechanics. The method is used for calculation of thick plates, plates of variable thickness, heterogeneous and multilayered plates. It is proposed to replace finite-difference relations by projection relations which will extend the potential of the method of lines and will allow using the method in the dynamic problems.

  15. Kansas Electric Transmission Lines

    Data.gov (United States)

    Kansas Data Access and Support Center — This data set is a digital representation of the EletcircTransmission lines for the State of Kansas as maintained by the Kansas Corporation Commission. Data is...

  16. Electric Power Transmission Lines

    Data.gov (United States)

    Department of Homeland Security — Transmission Lines are the system of structures, wires, insulators and associated hardware that carry electric energy from one point to another in an electric power...

  17. Superconducting metamaterial transmission line

    Science.gov (United States)

    Rouxinol, Francisco; Wang, Haozhi; Plourde, B. L. T.

    2014-03-01

    Left-handed metamaterials are artificial composite structures with unusual properties. Such systems have a wide range of potential applications in photonics. We are developing transmission lines composed of superconducting metamaterials using thin-film lumped circuit elements. Such structures allow for the possibility of generating novel transmission spectra with a high density of modes in some frequency ranges and stop-bands in others. We discuss possible couplings of these lines to superconducting qubits in circuit QED architectures.

  18. Creating product line architectures

    OpenAIRE

    Bayer, J.; Flege, O.; Gacek, C.

    2000-01-01

    The creation and validation of product line software architectures are inherently more complex than those of software architectures for single systems. This paper compares a process for creating and evaluating a traditional, one-of-a- kind software architecture with one for a reference software architecture. The comparison is done in the context of PuLSE-DSSA, a customizable process that integrates both product line architecture creation and evaluation.

  19. NLC Extraction Line Studies

    OpenAIRE

    Nosochkov, Y. M.; Raubenheimer, T. O.

    2001-01-01

    In this note, we briefly review the current lattice of the NLC extraction line which was designed for the nominal NLC beam parameters. Then we describe the beam parameters for the high luminosity option with larger beam disruption parameter and discuss its effect on beam loss in the extraction line. Finally, we present a summary of the optics study aimed at minimizing the beam loss with high disruption beams.

  20. On-Line and Off-Line Assessment of Metacognition

    Science.gov (United States)

    Saraç, Seda; Karakelle, Sema

    2012-01-01

    The study investigates the interrelationships between different on-line and off-line measures for assessing metacognition. The participants were 47 fifth grade elementary students. Metacognition was assessed through two off-line and two on-line measures. The off-line measures consisted of a teacher rating scale and a self-report questionnaire. The…

  1. Measurement of Deflection Line on Bridges

    Science.gov (United States)

    Urban, Rudolf; Štroner, Martin

    2013-12-01

    Prestressed concrete bridges are very sensitive to the increase in long-term deflections. Reliable forecasts of deflections of bridge structures during construction and durability are crucial for achieving good durability. The main results of measurements are the changes of the deflection line of the bridge structures, which places special demands on the measurement procedure. Results from measurements are very useful for the improvement of mathematical prediction methods of behaviour of long span prestressed concrete structures.

  2. Prospects for narrowing bounds on Earth's equilibrium climate sensitivity

    National Research Council Canada - National Science Library

    Stevens, Bjorn; Sherwood, Steven C; Bony, Sandrine; Webb, Mark J

    2016-01-01

    The concept of Earth's Equilibrium Climate Sensitivity ( ECS ) is reviewed. A particular problem in quantifying plausible bounds for ECS has been how to account for all of the diverse lines of relevant scientific evidence...

  3. Susceptibility of cell lines to avian viruses

    Directory of Open Access Journals (Sweden)

    Simoni Isabela Cristina

    1999-01-01

    Full Text Available The susceptibility of the five cell lines - IB-RS-2, RK-13, Vero, BHK-21, CER - to reovirus S1133 and infectious bursal disease virus (IBDV vaccine GBV-8 strain was studied to better define satisfactory and sensitive cell culture systems. Cultures were compared for presence of CPE, virus titers and detection of viral RNA. CPE and viral RNA were detected in CER and BHK-21 cells after reovirus inoculation and in RK-13 cell line after IBDV inoculation and with high virus titers. Virus replication by production of low virus titers occurred in IB-RS-2 and Vero cells with reovirus and in BHK-21 cell line with IBDV.

  4. Ethylene responses in three Hydrangea lines

    DEFF Research Database (Denmark)

    Lauridsen, Uffe Bjerre; Müller, Renate; Lütken, Henrik Vlk

    2015-01-01

    Abstract The ornamental scrub Hydrangea is generally not considered to be particularly sensitive to the phytohormone ethylene. The present study aimed at testing ethylene sensitivity in three different Hydrangea lines: 1, 2 and 3 taking into account the effect of temperature. Ethylene response...... was measured as leaf epinasty and leaf drop. Data indicated that higher temperature accelerates the effect of 2 μl L-1 ethylene over a 12-day period, and if the inhibitor 1-methylcyclopopene 1-MCP is able to attenuate this effect. Breeding line 1 and 3 dropped 3.8±0.6 and 5.0±0.4 leaves on average......, respectively, during the 12-day experimental period. Non-treated controls of line 1 and 3 dropped 1.8±0.6 and 1.8±0.4 leaves, respectively. In contrast, line 2 did not show a significant response to ethylene treatment with a leaf drop of 2.1±0.3 leaves, compared to a leaf drop of 0.8±0.3 in non...

  5. Along the line

    DEFF Research Database (Denmark)

    Dinesen, Cort Ross

    2011-01-01

    Embarking on a work of art constitutes a reduction of information – because we grasp the diversity and plurality of the manifestations we encounter by abstracting them and transforming them into manageable concepts; as we do when we draw contour lines on the landscape – they are imaginary...... and invisible but an abstraction essential for noting a difference or marking a place on a map. In the same way, a stroke in a sketch or a line in the sand is a manifestation of our ability to draw a boundary that both includes and excludes information. Where the line makes the birth of an idea visible......, it expresses through movement our tendency to mark a difference by drawing attention to and enclosing a whole series of relations that confront our preconceived notions. It is in the process of transformation in which we reduce the complexity of how we work, and rethink our ideas anew by refining the very same...

  6. Draw the Line!

    DEFF Research Database (Denmark)

    Draw the Line! International Conference, Copenhagen 2008 Papers, proceedings and recommendations is the second publication of the international three year long project "Understanding Puzzles in the Gendered European Map" (UPGEM), which is financed by the European Commission. In this publication we...... present proceedings from the UPGEM conference "Draw the Line!" in Copenhagen May 2008. The proceedings include contributions by UPGEM researchers, conference speeches by other researchers, politicians and gender equality officers in which they relate their work and research with the research done in UPGEM...... in the national contexts (see Draw the Line! Universities as workplaces for male and female researchers in Europe and http://www.upgem.dk/ ). We have also identified and discussed three scientific cultures in physics across the national borders. We call these scientific cultures: Hercules, Caretakers and Worker...

  7. VT Digital Line Graph Railroads

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) This datalayer is comprised of major railroad lines for the state of Vermont. These railroad lines line up well along quadrangle boundaries, but...

  8. Software product lines

    OpenAIRE

    Cortés Verdín, María Karen

    2005-01-01

    A Software Product Line is a “set of software-intensive systems sharing a common, managed set of features that satisfy the specific needs of a particular market segment or mission and that are developed from a common set of core assets in a prescribed way”1. A software product line (or software product family) approach promotes planned and proactive reuse of core assets and architecture-centric development, achieving a substantial increment in product quality and a reduced time to market. Bec...

  9. BPL (Broadband Power Line)

    OpenAIRE

    Peralta, Arturo

    2010-01-01

    El mundo actual requiere cada vez más el estar conectados con la sociedad de la Información y el Conocimiento, esto demanda que en los hogares, lugares de turismo, trabajos, universidades, etcétera, se cuente con una conexión a Internet de Alta Velocidad, que además sea segura y confiable. En este documento se presenta las características más importantes de la tecnología BPL (Broadband Power Line) o PLC (Power Line Comunication), la cual permite brindar el servicio de Internet ...

  10. Modeling the water line emission from the high-mass star-forming region AFGL 2591

    NARCIS (Netherlands)

    Poelman, D. R.; van der Tak, F. F. S.

    2007-01-01

    Context. Observations of water lines are a sensitive probe of the geometry, dynamics and chemical structure of dense molecular gas. The launch of Herschel with on board HIFI and PACS allows to probe the behaviour of multiple water lines with unprecedented sensitivity and resolution. Aims. We

  11. Absolute Poverty Lines

    DEFF Research Database (Denmark)

    Arndt, Channing; Mahrt, Kristi; Tarp, Finn

    2017-01-01

    ’ approach, employing multiple methods, is the best available mode for addressing these limitations. A first fundamental choice is whether to estimate an absolute poverty line at all. Consumption-based poverty metrics provide only a partial view into the welfare of individuals or households, which may or may...

  12. Bike Map Lines

    Data.gov (United States)

    Town of Chapel Hill, North Carolina — Chapel Hill Bike Map Lines from KMZ file.This data came from the wiki comment board for the public, not an “official map” showing the Town of Chapel Hill's plans or...

  13. Clamp Restrains Pressure Line

    Science.gov (United States)

    Aliberti, J. A.

    1982-01-01

    Safety restraint protects people and property if a high-pressure fitting fails. As long as pressure line remains attached at the fitting, clamp exerts essentially no force on hose. If fitting fails, force of fluid leaving free end of hose causes the cam on the clamp to compress hose with a positive locking action.

  14. Analysis of () Line Shape

    Indian Academy of Sciences (India)

    The line shape is also simulated by the Monte–Carlo method, the molecular dissociation contributes to 57% neutral atoms and 53% emission intensity in front of the limiter, and 85% neutral atoms and 82% emission intensity in front of the wall. The processes of atoms and molecules influence on the energy balance is ...

  15. Subjective poverty line definitions

    NARCIS (Netherlands)

    J. Flik; B.M.S. van Praag (Bernard)

    1991-01-01

    textabstractIn this paper we will deal with definitions of subjective poverty lines. To measure a poverty threshold value in terms of household income, which separates the poor from the non-poor, we take into account the opinions of all people in society. Three subjective methods will be discussed

  16. LEAR: antiproton extraction lines

    CERN Document Server

    Photographic Service

    1992-01-01

    Antiprotons, decelerated in LEAR to a momentum of 100 MeV/c (kinetic energy of 5.3 MeV), were delivered to the experiments in an "Ultra-Slow Extraction", dispensing some 1E9 antiprotons over times counted in hours. Beam-splitters and a multitude of beam-lines allowed several users to be supplied simultaneously.

  17. Automatic transmission line monitor

    Science.gov (United States)

    Parsons, W. E.; Richards, L. O.

    1971-01-01

    Monitor improves complex network reliability in computer data links and command transmission lines. System evaluates circuit performance against preselected criteria, identifies and stores data indicating out-of-tolerance conditions, conducts closed loop testing, and provides for operation under command of digital computer that determines restoration priorities.

  18. Drawing the Line

    DEFF Research Database (Denmark)

    Holm, Isak Winkel

    2016-01-01

    of judgement that distinguishes between the inside and outside of a political community, between citizens and zombies. Second, the chapter focuses on the rhetorics applied to draw the line between rights-bearers and the rightless. ‘Prophetic’ speech, echoing the language of the Old Testament prophets, is vital...

  19. Developing Green Line Products

    DEFF Research Database (Denmark)

    Muñoz-Marin, Ana Maria; Lassen, Astrid Heidemann; Poulsen, Søren Bolvig

    2011-01-01

    This publication is based on the Master thesis “User-driven ecoinnovation process: Towards the implementation of the Green product line at JELD-WEN” written by Ana Maria Muñoz-Marin as her Graduation Project for the MSc. Global Innovation Management degree. The company-based experiment was carried...

  20. America's Assembly Line

    DEFF Research Database (Denmark)

    Nye, David Edwin

    A social history of the assembly line, invented in 1913. Both praised as a boon to consumers and as a curse for workers, it has been satirized, imitated, and celebrated for 100 years. It has inspired fiction, comedy, cafeteria layouts, and suburban housing. It transformed industrial labor...

  1. Insulin sensitivity and albuminuria

    DEFF Research Database (Denmark)

    Pilz, Stefan; Rutters, Femke; Nijpels, Giel

    2014-01-01

    OBJECTIVE: Accumulating evidence suggests an association between insulin sensitivity and albuminuria, which, even in the normal range, is a risk factor for cardiovascular diseases. We evaluated whether insulin sensitivity is associated with albuminuria in healthy subjects. RESEARCH DESIGN...... albuminuria. This finding suggests that reduced insulin sensitivity either is simply related to or might causally contribute to the initial pathogenesis of albuminuria....

  2. Literature and Social Sensitivity.

    Science.gov (United States)

    Loban, Walter

    Focusing on the responses of adolescents to literature "involving values based on the concept of human dignity," this study examined two groups of adolescents (60 students each) in grades 8-12. The two groups represented extremes of social sensitivity: highly sensitive and least sensitive to the feelings of others. Teachers read aloud 10 short…

  3. Local sensitivity in econometrics

    NARCIS (Netherlands)

    Vasnev, A.L.

    2006-01-01

    The study of the effect of the violations of the model assumptions on the parameter of interest is called sensitivity analysis. Chapter 2 of this thesis defines the sensitivity statistic and confronts sensitivity analysis with diagnostic testing in a general maximum likelihood framework. Under

  4. Zebrafish Sensitivity to Botulinum Neurotoxins

    Science.gov (United States)

    Chatla, Kamalakar; Gaunt, Patricia S.; Petrie-Hanson, Lora; Ford, Lorelei; Hanson, Larry A.

    2016-01-01

    Botulinum neurotoxins (BoNT) are the most potent known toxins. The mouse LD50 assay is the gold standard for testing BoNT potency, but is not sensitive enough to detect the extremely low levels of neurotoxin that may be present in the serum of sensitive animal species that are showing the effects of BoNT toxicity, such as channel catfish affected by visceral toxicosis of catfish. Since zebrafish are an important animal model for diverse biomedical and basic research, they are readily available and have defined genetic lines that facilitate reproducibility. This makes them attractive for use as an alternative bioassay organism. The utility of zebrafish as a bioassay model organism for BoNT was investigated. The 96 h median immobilizing doses of BoNT/A, BoNT/C, BoNT/E, and BoNT/F for adult male Tübingen strain zebrafish (0.32 g mean weight) at 25 °C were 16.31, 124.6, 4.7, and 0.61 picograms (pg)/fish, respectively. These findings support the use of the zebrafish-based bioassays for evaluating the presence of BoNT/A, BoNT/E, and BoNT/F. Evaluating the basis of the relatively high resistance of zebrafish to BoNT/C and the extreme sensitivity to BoNT/F may reveal unique functional patterns to the action of these neurotoxins. PMID:27153088

  5. Zebrafish Sensitivity to Botulinum Neurotoxins

    Directory of Open Access Journals (Sweden)

    Kamalakar Chatla

    2016-05-01

    Full Text Available Botulinum neurotoxins (BoNT are the most potent known toxins. The mouse LD50 assay is the gold standard for testing BoNT potency, but is not sensitive enough to detect the extremely low levels of neurotoxin that may be present in the serum of sensitive animal species that are showing the effects of BoNT toxicity, such as channel catfish affected by visceral toxicosis of catfish. Since zebrafish are an important animal model for diverse biomedical and basic research, they are readily available and have defined genetic lines that facilitate reproducibility. This makes them attractive for use as an alternative bioassay organism. The utility of zebrafish as a bioassay model organism for BoNT was investigated. The 96 h median immobilizing doses of BoNT/A, BoNT/C, BoNT/E, and BoNT/F for adult male Tübingen strain zebrafish (0.32 g mean weight at 25 °C were 16.31, 124.6, 4.7, and 0.61 picograms (pg/fish, respectively. These findings support the use of the zebrafish-based bioassays for evaluating the presence of BoNT/A, BoNT/E, and BoNT/F. Evaluating the basis of the relatively high resistance of zebrafish to BoNT/C and the extreme sensitivity to BoNT/F may reveal unique functional patterns to the action of these neurotoxins.

  6. Directed Line Liquids

    Science.gov (United States)

    Kamien, Randall David

    This thesis is devoted to the study of ensembles of dense directed lines. These lines are principally to be thought of as polymers, though they also have the morphology of flux lines in high temperature superconductors, strings of colloidal spheres in electrorheological fluids and the world lines of quantum mechanical bosons. We first study nematic polymers dissolved in a nematic solvent. We show that in the dense phase nematic polymers interacting directly through excluded volume and indirectly through nematic solvent fluctuations are, at long wavelengths, identical to nematic polymers in an isotropic solvent, interacting through, again, excluded volume as well as their nematic degrees of freedom. By including the effects of free ends we study ferro- and electrorheological fluids and finite chain length polymers. In the dilute phase, near the transition to the semi-dilute phase, a renormalization group treatment is performed, in the physical and critical dimension 3. For sufficiently dilute systems we find logarithmic corrections to polymer wandering, predicted by de Gennes. However, at higher densities, the logarithmic enhancement is suppressed, and the polymers execute conventional random walks along the preferred axis. We then study the isotropic to nematic transition in this same system of liquid-crystalline polymers. Using the replica technique introduced by de Gennes to study isotropic polymers, we formulate a fully isotropic theory of liquid-crystalline polymers dissolved in a liquid crystal matrix. The first order transition from isotropic to nematic ordering occurs, and leads to the original directed polymer theory previously studied. In agreement with physical expectations, the nematic ordering temperature is depressed by polymerization. Finally, we go back to directed systems, in this case directed by an explicit symmetry violation instead of spontaneously. We discuss how directed polymer melts, string-like formations in electrorheological and ferro

  7. A Quantitative Transmission Line Experiment

    Science.gov (United States)

    Johnston, D. C.; Silbernagel, B. G.

    1969-01-01

    Describes modifications of a commercially available strip-type transmission line, which makes possible reproducible measurements of standing waves on the line. Experimental data yield values for the characteristic impedance, phase velocity and line wavelength of radiation in the transmission line, and the dielectric constant of material in the…

  8. The Bicycle Assembly Line Game

    Science.gov (United States)

    Klotz, Dorothy

    2011-01-01

    "The Bicycle Assembly Line Game" is a team-based, in-class activity that helps students develop a basic understanding of continuously operating processes. Each team of 7-10 students selects one of seven prefigured bicycle assembly lines to operate. The lines are run in real-time, and the team that operates the line that yields the…

  9. MDR Gene Expression Analysis of Six Drug-Resistant Ovarian Cancer Cell Lines

    Directory of Open Access Journals (Sweden)

    Radosław Januchowski

    2013-01-01

    and protein levels. Cell lines resistant to agents used in ovarian cancer treatment remained sensitive to methotrexate. The main mechanisms of drug resistance were due to P-gp expression in the doxorubicin, vincristine, and paclitaxel resistant cell lines and BCRP expression in the topotecan resistant cell line.

  10. Printed circuit dispersive transmission line

    Science.gov (United States)

    Ikezi, Hiroyuki; Lin-Liu, Yuh-Ren; DeGrassie, John S.

    1991-01-01

    A printed circuit dispersive transmission line structure is disclosed comprising an insulator, a ground plane formed on one surface of the insulator, a first transmission line formed on a second surface of the insulator, and a second transmission line also formed on the second surface of the insulator and of longer length than the first transmission line and periodically intersecting the first transmission line. In a preferred embodiment, the transmission line structure exhibits highly dispersive characteristics by designing the length of one of the transmission line between two adjacent periodic intersections to be longer than the other.

  11. Fuel line purging device

    Energy Technology Data Exchange (ETDEWEB)

    Hensel, R.J.

    1988-03-22

    In a fuel injection system for an internal combustion engine having a fuel pump communicating with a fuel tank and delivering pressurized fuel through a pressure line to a fuel injector, the improvement is described comprising purging means for purging gas, including air and vapor, from the system, comprising: a housing having an inlet communicating with the pressure line, and having an outlet; pressure responsive valve means in the housing movable between an open position permitting flow from the inlet to the outlet, and a closed position blocking flow from the inlet to the outlet; a first branch passage in the housing communicating between the inlet and one side of the valve means; a second branch passage in the housing communicating between the inlet and the other side of the valve means; restriction means in the second branch passage selected to provide a fuel pressure drop thereacross but not substantially dropping gas pressure thereacross.

  12. Adaptive method of lines

    CERN Document Server

    Saucez, Ph

    2001-01-01

    The general Method of Lines (MOL) procedure provides a flexible format for the solution of all the major classes of partial differential equations (PDEs) and is particularly well suited to evolutionary, nonlinear wave PDEs. Despite its utility, however, there are relatively few texts that explore it at a more advanced level and reflect the method''s current state of development.Written by distinguished researchers in the field, Adaptive Method of Lines reflects the diversity of techniques and applications related to the MOL. Most of its chapters focus on a particular application but also provide a discussion of underlying philosophy and technique. Particular attention is paid to the concept of both temporal and spatial adaptivity in solving time-dependent PDEs. Many important ideas and methods are introduced, including moving grids and grid refinement, static and dynamic gridding, the equidistribution principle and the concept of a monitor function, the minimization of a functional, and the moving finite elem...

  13. Draw the Line!

    DEFF Research Database (Denmark)

    present proceedings from the UPGEM conference "Draw the Line!" in Copenhagen May 2008. The proceedings include contributions by UPGEM researchers, conference speeches by other researchers, politicians and gender equality officers in which they relate their work and research with the research done in UPGEM......Draw the Line! International Conference, Copenhagen 2008 Papers, proceedings and recommendations is the second publication of the international three year long project "Understanding Puzzles in the Gendered European Map" (UPGEM), which is financed by the European Commission. In this publication we....... The UPGEM research has previously been published in national reports from each of the partner countries (Denmark, Estonia, Finland, Italy and Poland). In these individual national reports of universities as workplaces for male and female researchers we discuss scientific cultures in physics embedded...

  14. Drill string transmission line

    Science.gov (United States)

    Hall, David R.; Hall, Jr., H. Tracy; Pixton, David S.; Bradford, Kline; Fox, Joe

    2006-03-28

    A transmission line assembly for transmitting information along a downhole tool comprising a pin end, a box end, and a central bore traveling between the pin end and the box end, is disclosed in one embodiment of the invention as including a protective conduit. A transmission line is routed through the protective conduit. The protective conduit is routed through the central bore and the ends of the protective conduit are routed through channels formed in the pin end and box end of the downhole tool. The protective conduit is elastically forced into a spiral or other non-linear path along the interior surface of the central bore by compressing the protective conduit to a length within the downhole tool shorter than the protective conduit.

  15. Hydrogen Stark Broadened Brackett lines

    Directory of Open Access Journals (Sweden)

    C. Stehlé

    2010-01-01

    Full Text Available Stark-broadened lines of the hydrogen Brackett series are computed for the conditions of stellar atmospheres and circumstellar envelopes. The computation is performed within the Model Microfield Method, which includes the ion dynamic effects and makes the bridge between the impact limit at low density and the static limit at high density and in the line wings. The computation gives the area normalized line shape, from the line core up to the static line wings.

  16. Line managers as marketers.

    Science.gov (United States)

    Rynne, T J

    1986-09-01

    Many hospital administrators and boards of directors, having overcome their initial opposition to advertising and hired a marketing professional, are wondering why their marketing programs have not accomplished all that they had hoped. The answer lies in their perception of who is responsible for marketing. Marketing is not a specialty separate from the disciplines of management--it is a basic management skill. Its goal is to satisfy the customer by providing products and services that meet the customer's needs, wants, and preferences. Line managers must understand the hospital's customers and make the critical day-to-day differences in satisfying them. They are the hospital's front-line marketers, and the marketing department's role is to support them in this function. The marketing department should develop the line managers' marketing skills by furnishing them with the appropriate tools: formats, models, examples, and instructions. In addition, the marketing staff should provide such specialized services as marketing research, ad agency management, and development of communications.

  17. Evaluation of some bean lines tolerance to alkaline soil

    Directory of Open Access Journals (Sweden)

    Abeer A. Radi

    2012-01-01

    Full Text Available Introduction: In less arid climates, salts are less concentrated and sodium dominates in carbonate and bicarbonate forms, which enhance the formation of alkaline soils. The development and identification of salt-tolerant crop cultivars or lines would complement salt management programs to improve the productivity and yields of salt stressed plants.Materials and methods: This work was to study the evaluation of alkalinity tolerance of some bean lines grown under different levels of sodium carbonate (Na2CO3 to select the most alkalinity tolerant lines versus the most-sensitive ones out of 6 lines of the test plants.Results: The symptoms induced by alkalinity included reduction in root, shoot growth, and leaf area which were more severe in some bean lines. Potassium leakage was severely affected by alkalinity in some lines at all tested levels, while in some others a moderate damage was manifested only at the higher levels. The increase in Na2CO3 level was associated with a gradual fall in chlorophyll a and b biosynthesis of all the test bean lines. However, alkalinity at low and moderate levels had a favorable effect on the biosynthesis of carotenoids in all the test bean lines. The increase in Na2CO3 supply had a considerable stimulatory effect on sodium accumulation, while potassium accumulation fluctuated in organs of bean lines.Conclusion: Assiut 1104 out of all the different lines investigated was found to display the lowest sensitivity to alkalinity stress, while Assiut 12/104 was the most sensitive one.

  18. Fabrics Protect Sensitive Skin from UV Rays

    Science.gov (United States)

    2009-01-01

    Late Johnson Space Center engineer Dr. Robert Dotts headed a team to develop cool suits for children suffering from life-threatening sun sensitivities. Dotts hoped to develop ultraviolet-blocking technology in a fabric that -- unlike in a bulky space suit -- could remain comfortable, light, and breathable in the sun and heat. The team worked with SPF 4 US LLC (SPF) of Madison, Wisconsin to design ultraviolet-blocking cool suits, which protect sun-sensitive patients and enable them to experience life outdoors safely. Using knowledge gained during the NASA collaboration, SPF created an entire line of ultraviolet-blocking apparel.

  19. Sensitivity and uncertainty analysis

    CERN Document Server

    Cacuci, Dan G; Navon, Ionel Michael

    2005-01-01

    As computer-assisted modeling and analysis of physical processes have continued to grow and diversify, sensitivity and uncertainty analyses have become indispensable scientific tools. Sensitivity and Uncertainty Analysis. Volume I: Theory focused on the mathematical underpinnings of two important methods for such analyses: the Adjoint Sensitivity Analysis Procedure and the Global Adjoint Sensitivity Analysis Procedure. This volume concentrates on the practical aspects of performing these analyses for large-scale systems. The applications addressed include two-phase flow problems, a radiative c

  20. [Study of thromboplastin sensitivity].

    Science.gov (United States)

    Kachalova, N D; Prostakova, T M; Kozlov, A A

    2001-03-01

    Comparative analysis of foreign and Russian thromboplastins demonstrated differences in the notions "activity" and "sensitivity" and the significance of low value of International Index of Sensitivity (IIS). Sensitivity of thromboplastin manufactured by RENAM Firm, Russia (IIS = 1.5) to deficiency of prothrombin factors II, VII, X has been demonstrated, which means that this thromboplastin can be used for diagnosis of deficiency of each of these factors. The authors consider that testing of thromboplastin sensitivity to factor VII is the main criterion of its fitness for monitoring anticoagulant therapy.

  1. Signal processors for position-sensitive detectors

    Energy Technology Data Exchange (ETDEWEB)

    Hasegawa, Ken-ichi [Hosei Univ., Koganei, Tokyo (Japan). Coll. of Engineering

    1996-07-01

    Position-sensitive detectors (PSD) are widely used in following various fields: condensed matter studies, material engineering, medical radiology particle physics, astrophysics and industrial applications. X-ray diffraction analysis is one of the field where PSDs are the most important instruments. In this field, many types of PSAs are employed: position-sensitive proportional counters (PSPC), multi-wire proportional chambers (MWPC), imaging plates, image intensifiers combined CCD cameras and semiconductor array devices. Two readout systems used for PSDs, where one is a charge-division type with high stability and the other is an encoder with multiple delay, line readout circuits useful for fast counting, were reported in this paper. The multiple delay line encoding system can be applicable to high counting rate 1D and 2D gas proportional detectors. (G.K.)

  2. LINE BALANCING IN SERVICES

    Directory of Open Access Journals (Sweden)

    Hadi GÖKÇEN

    2002-01-01

    Full Text Available This paper investigates how the Assembly Line Balancing (ALB methods known for the production systems can be applied in service systems. For this purpose, an application study is done in a Physical therapy and rehabilitation clinic. Each standart service provided to patients is taken into consideration as a product, and problem is solved as a mixed model ALB problem. In conclusion, it is seen that the ALB methods developed for the production systems are also applicable in service systems. So, it is possible to do similar studies at hospitals, tax offices, driving licence and passport offices, fast food restaurants, etc.

  3. America's Assembly Line

    DEFF Research Database (Denmark)

    Nye, David Edwin

    A social history of the assembly line, invented in 1913. Both praised as a boon to consumers and as a curse for workers, it has been satirized, imitated, and celebrated for 100 years. It has inspired fiction, comedy, cafeteria layouts, and suburban housing. It transformed industrial labor...... and provoked strikes and union drives in the 1930s, but became a symbol of victory in the Second World War and Cold War. Reinvented by Japan as "lean production" and then increasingly automated after 1990, it remains a cornerstone of production but no longer employs many workers, even as it evolves toward...

  4. From Off-line to On-line Handwriting Recognition

    NARCIS (Netherlands)

    Lallican, P.; Viard-Gaudin, C.; Knerr, S.

    2004-01-01

    On-line handwriting includes more information on time order of the writing signal and on the dynamics of the writing process than off-line handwriting. Therefore, on-line recognition systems achieve higher recognition rates. This can be concluded from results reported in the literature, and has been

  5. Assessing Sensitiveness to Transport

    DEFF Research Database (Denmark)

    Lieb, Christoph; Suter, Stefan; Sánchez, Alfredo

    Summary The EU-project ASSET (ASessing SEnsitiveness to Transport) aims at developing and implementing a concise concept to assess transport sensitive areas (TSA) in a European context, i.e. areas in which transport leads to more serious impacts than in other areas. The aim of work package 2 (WP2...

  6. Structure sensitivity in adsorption

    DEFF Research Database (Denmark)

    Hammer, Bjørk; Nielsen, Ole Holm; Nørskov, Jens Kehlet

    1997-01-01

    The structure sensitivity of CO adsorption on different flat, stepped, kinked and reconstructed Pt surfaces is studied using large-scale density-functional calculations. We find an extremely strong structure sensitivity in the adsorption energy with variations up to 1 eV (or 100%) from one...

  7. Strategic production line synchronisation

    Directory of Open Access Journals (Sweden)

    Hattingh, Teresa

    2016-08-01

    Full Text Available A study was conducted at the sole global producer of suspension struts for a particular vehicle manufacturer. This supplier is currently able to meet customer demand. However, it does so because of a large finished goods and work-in-progress (WIP inventory. The plant operates two production processes that are separated by a large buffer of WIP, which essentially decouples the production processes. This study aimed to reduce this WIP buffer; this would require the processes to become synchronised, bearing in mind that the reliability of delivery should not decrease. A tool that considers time, quality, and machine capacity was developed to assess the impact of line synchronisation on company performance figures. It was found that line synchronisation produced several benefits for the supplier, including batch size reduction, lower inventory levels, and associated shorter lead times. This further allowed the supplier to improve flow in the plant by introducing a pull system. Improved visual oversight could lead to further improved problem-solving and innovation.

  8. Finnish Teachers’ Ethical Sensitivity

    Directory of Open Access Journals (Sweden)

    Elina Kuusisto

    2012-01-01

    Full Text Available The study examined the ethical sensitivity of Finnish teachers (=864 using a 28-item Ethical Sensitivity Scale Questionnaire (ESSQ. The psychometric qualities of this instrument were analyzed, as were the differences in self-reported ethical sensitivity between practicing and student teachers and teachers of different subjects. The results showed that the psychometric qualities of the ESSQ were satisfactory and enabled the use of an explorative factor analysis. All Finnish teachers rated their level of ethical sensitivity as high, which indicates that they had internalized the ethical professionalism of teaching. However, practicing teachers’ assessments were higher than student teachers’. Moreover, science as a subject was associated with lower self-ratings of ethical sensitivity.

  9. Sensitive skin: An overview

    Directory of Open Access Journals (Sweden)

    Arun C Inamadar

    2013-01-01

    Full Text Available Sensitive skin is less tolerant to frequent and prolonged use of cosmetics and toiletries. It is self-diagnosed and typically unaccompanied by any obvious physical signs of irritation. With the change in lifestyle and also with increased opportunity to use many new brands of cosmetics and toiletries, there has been an increase in females complaining of unique sensation in their facial skin. Sensitive skin presents as smarting, burning, stinging, itching, and/or tight sensation in their facial skin. The condition is found in more than 50% of women and 40% of men, creating a sizable demand for products designed to minimize skin sensitivity. Good numbers of invasive and non-invasive tests are designed to evaluate and predict the sensitive skin. Management includes guidelines for selecting suitable cosmetics and toiletries in sensitive skin individuals.

  10. Sensitive skin: an overview.

    Science.gov (United States)

    Inamadar, Arun C; Palit, Aparna

    2013-01-01

    Sensitive skin is less tolerant to frequent and prolonged use of cosmetics and toiletries. It is self-diagnosed and typically unaccompanied by any obvious physical signs of irritation. With the change in lifestyle and also with increased opportunity to use many new brands of cosmetics and toiletries, there has been an increase in females complaining of unique sensation in their facial skin. Sensitive skin presents as smarting, burning, stinging, itching, and/or tight sensation in their facial skin. The condition is found in more than 50% of women and 40% of men, creating a sizable demand for products designed to minimize skin sensitivity. Good numbers of invasive and non-invasive tests are designed to evaluate and predict the sensitive skin. Management includes guidelines for selecting suitable cosmetics and toiletries in sensitive skin individuals.

  11. Breastfeeding, sensitivity, and attachment.

    Science.gov (United States)

    Britton, John R; Britton, Helen L; Gronwaldt, Virginia

    2006-11-01

    Our goal was to test the hypothesis that breastfeeding is associated with enhanced infant-mother attachment and its antecedent maternal sensitivity. Breastfeeding intent and practice were assessed by questionnaires administered to 152 mothers between 32 weeks of gestation and 12 months postpartum. Early maternal sensitivity was measured by the Sensitivity to Cues subscale of the Nursing Child Assessment Satellite Training Feeding Scale at 3 months, and quality of the mother-infant interaction was measured by the Nursing Child Assessment Satellite Training Feeding Scale at 6 months. Security of attachment was evaluated by the Ainsworth Strange Situation at 12 months. A direct relationship between attachment security and breastfeeding practice was not identified. The quality of the mother-infant interaction at 6 months, rather than the type of feeding, predicted security of attachment. However, mothers who chose to breastfeed displayed greater sensitivity in dyadic interactions with their infants 3 months postnatally than those who chose to bottle feed, and intended breastfeeding duration prenatally correlated with sensitivity 3 months postpartum. Although a path analysis failed to demonstrate contributions of early breastfeeding duration to either sensitivity or security, it did substantiate a significant path between prenatal breastfeeding intent and attachment security mediated by sensitivity. In addition, multivariate analyses revealed that early sensitivity among breastfeeding mothers was an independent predictor of the duration of any and exclusive breastfeeding during the first year. Although the quality of the dyadic interaction in infancy, rather than feeding type, is predictive of attachment security, mothers who choose to breastfeed display enhanced sensitivity during early infancy that, in turn, may foster secure attachment. Among breastfeeding mothers, higher sensitivity is associated with longer duration of breastfeeding during the first postpartum year

  12. Central Venous Catheter (Central Line)

    Science.gov (United States)

    ... venous catheter (KATHeter), also known as a central line or CVC, is long, soft, thin, hollow tube ... into a large vein (blood vessel). A central line is much like an intravenous (IV) catheter that ...

  13. Dual Band High Efficiency Power Amplifier Based on CRLH Lines

    Directory of Open Access Journals (Sweden)

    D. Segovia-Vargas

    2009-12-01

    Full Text Available In this paper we propose the use of Composite Right/Left Hand (CRLH and Extended Composite Right/Left Hand (ECRLH transmission lines for the design of dual band high efficiency power amplifiers working in CE class. The harmonic termination can be synthesized using the meta-lines is particularly suitable for CE class amplifiers, which have a termination not as sensitive to the third harmonic as F class amplifier. This paper presents the design procedure and the design equations. The nonlinear phase response of a CRLH and ECRLH transmission line has been utilized to design arbitrary dual-band amplifiers.

  14. Credit Lines and Credit Utilization

    OpenAIRE

    Agarwal, Sumit; Ambrose, Brent W.; Liu, Chunlin

    2006-01-01

    While much is known about the characteristics of consumers or businesses that obtain credit lines, relatively little is known empirically about credit line utilization after origination. This study fills that gap by testing two interrelated hypotheses concerning borrower credit quality and credit line utilization. The empirical analysis confirms that borrowers with higher expectations of future credit quality deterioration originate credit lines to preserve financial flexibility. Furthermore,...

  15. China's Bilateral Currency Swap Lines

    OpenAIRE

    Zhitao, Lin; Wenjie, Zhan; Cheung, Yin-Wong

    2016-01-01

    We study the determinants of China’s bilateral local currency swap lines that were established since the recent global finance crisis. It is found that economic factors, political considerations, and institutional characteristics including trade intensity, economic size, strategic partnership, free trade agreement, corruption, and stability affect the decision of signing a swap line agreement. Once a swap line agreement decision is made, the size of the swap line is then mainly affected by tr...

  16. The potential effectiveness of nanoparticles as radio sensitizers for radiotherapy

    Directory of Open Access Journals (Sweden)

    Mohammad Babaei

    2014-03-01

    Full Text Available Introduction: Application of nanoparticles as radio sensitizer is a promising field to improve efficiency of radiotherapy.Methods:This study was conducted to review nano radio sensitizers. PubMed, Ovid Medline, Science Direct, Scopus, ISI web of knowledge, and Springer databases were searched from 2000 to May 2013 to identify relevant studies. Search was restricted to English language. Results: We included any study that evaluated nanoparticles, volunteer of radio enhancement at radiotherapy on animals or cell lines. Nanoparticles can increase radio sensitivity of tumor cells. This effect was shown in vivo and in vitro, at kilovltage or megavoltage energies, in 24 reviewed studies. Focus of studies was on gold nanoparticles. Radio sensitizing effects of nanoparticles depend on nanoparticles’ size, type, concentration, intracellular localization, used irradiation energy and tested cell line.Conclusion: Literature suggests potency of nanoparticles for increasing cell radio sensitivity. Reviewed results are promising and warrant future clinical trials.

  17. Protease-sensitive synthetic prions.

    Directory of Open Access Journals (Sweden)

    David W Colby

    2010-01-01

    Full Text Available Prions arise when the cellular prion protein (PrP(C undergoes a self-propagating conformational change; the resulting infectious conformer is designated PrP(Sc. Frequently, PrP(Sc is protease-resistant but protease-sensitive (s prions have been isolated in humans and other animals. We report here that protease-sensitive, synthetic prions were generated in vitro during polymerization of recombinant (rec PrP into amyloid fibers. In 22 independent experiments, recPrP amyloid preparations, but not recPrP monomers or oligomers, transmitted disease to transgenic mice (n = 164, denoted Tg9949 mice, that overexpress N-terminally truncated PrP. Tg9949 control mice (n = 174 did not spontaneously generate prions although they were prone to late-onset spontaneous neurological dysfunction. When synthetic prion isolates from infected Tg9949 mice were serially transmitted in the same line of mice, they exhibited sPrP(Sc and caused neurodegeneration. Interestingly, these protease-sensitive prions did not shorten the life span of Tg9949 mice despite causing extensive neurodegeneration. We inoculated three synthetic prion isolates into Tg4053 mice that overexpress full-length PrP; Tg4053 mice are not prone to developing spontaneous neurological dysfunction. The synthetic prion isolates caused disease in 600-750 days in Tg4053 mice, which exhibited sPrP(Sc. These novel synthetic prions demonstrate that conformational changes in wild-type PrP can produce mouse prions composed exclusively of sPrP(Sc.

  18. Action spectra (254-302 nm) for four human photosensitive cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Coohill, T.P.; Moore, S.P.; Grider, R.A. (Western Kentucky Univ., Bowling Green (USA))

    1983-07-01

    The inactivation of cellular viral capacity for Herpes simplex type I growth in six separate wavelengths (254-302 nm) was measured in five human cell lines. These consisted of one ''normal''-skin fibroblast line (KD), and four photosensitive lines. Two lines of Xeroderma pigmentosum, one of Bloom's syndrome, and one of Cockayne's syndrome cells were used. Similar relative sensitivity were observed for the Bloom's syndrome. Xeroderma pigmentosum, and normal cell lines. The Cockayne's syndrome-line became relatively more sensitive at 289 nm and longer wavelengths. Absolute sensitivities varied. Some divergence in response was noted at the longest wavelength tested, 302 nm.

  19. Software Product Lines : Three Examples

    OpenAIRE

    2000-01-01

    This report presents the results of a course on software product lines. The assignment in the course was to develop a software product line. . During the course, the students had to develop the following artifacts in groups: product line architecture, component designs, product derivations, modifiability assessment and a prototype. The results of the course are described in this report

  20. The Median-Median Line

    Science.gov (United States)

    Wilson, David C.

    2010-01-01

    Graphing bivariate data in a scatter plot and drawing an approximate line of best fit for the data have become commonly recommended activities for middle school and high school students. The graphing calculator has provided a mechanism for students both to approximate a best-fit line and to calculate the best-fit line using a built-in option. Two…

  1. [Spectral line shift property of prism dispersive imaging spectrometer].

    Science.gov (United States)

    Zhang, Jun-qiang; Yan, Chang-xiang; Zheng, Yu-quan; Wu, Qing wen

    2011-12-01

    In order to study the spectral line shift property of prism-dispersive imaging spectrometer, the influencing factors and mechanisms of spectral line shift were presented, and the mathematical model based on linear optics model was established to describe the spectral line shift property. Code V API functions was used, in Matlab environment, to verify the validity of mathematical model, and the sensitivity coefficient of spectral line shift was analyzed. Results indicate that rigid body motion of optical mirror surface generated by environmental variation is the key causation of spectral line shift. When the decenter of mirror surface is no more than 0.2 mm and the tilt is less than 0.02 degrees, the value of spectral line shift of different wavelengths at different fields is equivalent, and the error is less than 0.1 pixel. Spectral line shift due to mirror rigid body motion is linear and independent, and the total shift of the spectral line is the algebraic sum of values produced by the single freedom of motion (DOF) of single mirror surface. The mathematical model based on linear optics model can be used to study the spectral line shift property of the prism-dispersive imaging spectrometer. It will provide some guidance for spectral calibration and spectral property analysis under complex work condition.

  2. Transgenic Campanula carpatica plants with reduced ethylene sensitivity.

    Science.gov (United States)

    Sriskandarajah, Sridevy; Mibus, Heiko; Serek, Margrethe

    2007-06-01

    Fertile transgenic Campanula carpatica Jacq. plants with flowers, which had reduced sensitivity to ethylene were obtained by Agrobacterium tumefaciens that mediated transformation. The construct used for transformation contained the etr1-1 gene from Arabidopsis thaliana under control of the flower specific fbp1-promoter from petunia. More than 100 flowering T0 lines were tested for their ethylene sensitivity using 2 microl l(-1) ethylene. The tolerance level to ethylene varied among the lines. While control plants stopped flowering within 3 days of exposure to ethylene, one of the transformed lines flowered for up to 27 days. The presence and the expression pattern of the transgene in various tissues were studied by polymerase chain reaction (PCR) and reverse transcription (RT)-PCR techniques. The expression of etr1-1 was significant in flowers and buds. Transgenic lines did not differ morphologically from control plants. The selected transgenic T0 lines, which were re-established from in vitro cultures showed the same degree of tolerance to exogenous ethylene, confirming the stability of the transgene in in vitro cultures. The rooting ability of the transgenic plants was not affected by the presence of etr1-1. T1 progeny were produced by crossing the transgenic line, which showed the most significant reduction in ethylene sensitivity with a control plant, and the analysis of the T1 plants showed 1:1 segregation in terms of ethylene sensitivity and the presence of the transgene.

  3. Lines of landscape organisation

    DEFF Research Database (Denmark)

    Løvschal, Mette

    2015-01-01

    This paper offers a landscape analysis of the earliest linear landscape boundaries on Skovbjerg Moraine, Denmark, during the first millennium BC. Using Delaunay triangulation as well as classic distribution analyses, it demonstrates that landscape boundaries articulated already established use......-patterns close to roads, but also intercepted the central lines of movement and conflicted with previous ways of organizing the landscape. This development is interpreted as a different form of large-scale landholding, in which livestock possibly played a dominant role and boundaries were used to confiscate land...... in the zones bordering suitable pastures. This situation shows obvious parallels with southern Britain centuries earlier. It is discussed how the study of these physical boundaries provides new insights into the organization of pre-Roman landscapes, not only demonstrating a continuing engagement with landscape...

  4. GPX3 promoter methylation predicts platinum sensitivity in colorectal cancer.

    Science.gov (United States)

    Pelosof, Lorraine; Yerram, Sashidhar; Armstrong, Todd; Chu, Nina; Danilova, Ludmila; Yanagisawa, Breann; Hidalgo, Manuel; Azad, Nilofer; Herman, James G

    2017-07-03

    Epigenetic control of gene expression is a major determinant of tumor phenotype and has been found to influence sensitivity to individual chemotherapeutic agents. Glutathione peroxidase 3 (GPX3, plasma glutathione peroxidase) is a key component of cellular antioxidant regulation and its gene has been reported to be methylated in specific tumor types. GPX3 role in oxidative damage has been associated with sensitivity to platinums in other tumors but its importance in colorectal cancer (CRC) has not been determined. We examined the role of GPX3 methylation in colorectal carcinoma in determining sensitivity to platinum drugs using primary tumor specimens, cell lines, knockdown cell lines, and tumor cell line xenografts. We find GPX3 promoter region methylation in approximately one third of CRC samples and GPX3 methylation leads to reduced GPX3 expression and increased oxaliplatin and cisplatin sensitivity. In contrast, in cell lines with high baseline levels of GPX3 expression or with the ability to increase GPX3 expression, platinum resistance is increased. The cisplatin IC50 in GPX3-methylated cell lines is approximately 6-fold lower than that in GPX3-unmethylated lines. Additionally, knockdown cell lines with essentially no GPX3 expression require N-acetylcysteine to survive in culture underscoring the importance of GPX3 in redox biology. In vivo, GPX3 methylation predicts tumor xenograft sensitivity to platinum with regression of GPX3 knockdown xenografts with platinum treatment but continued growth of GPX3 wild type xenografts in the presence of platinum. These studies demonstrate the importance of GPX3 for CRC cells resistance to platinums and the potential utility of GPX3 methylation status as a predictive biomarker for platinum sensitivity in CRC.

  5. RPC gamma sensitivity simulation

    CERN Document Server

    Altieri, S; Bruno, G; Gianini, G; Merlo, M; Ratti, S P; Riccardi, C; Torre, P; Viola, L; Vitulo, P; Abbrescia, M; Colaleo, A; Iaselli, Giuseppe; Loddo, F; Maggi, M; Marangelli, B; Natali, S; Nuzzo, S; Pugliese, G; Ranieri, A; Romano, F; Salvemini, A

    2000-01-01

    A method to simulate resistive plate chambers (RPCs) gamma sensitivity has been developed using a Monte Carlo code. The sensitivity has been evaluated as a function of the gamma energy in the range 0.1-100 MeV and for different spectra. To evaluate the response of the detector in a Large Hadron Collider (LHC) background environment the gamma energy spectrum expected in the CMS muon barrel has been taken into account and the RPC gamma sensitivity evaluated as a function of the detector size. (3 refs).

  6. Comprehensive analysis of line-edge and line-width roughness for EUV lithography

    Science.gov (United States)

    Bonam, Ravi; Liu, Chi-Chun; Breton, Mary; Sieg, Stuart; Seshadri, Indira; Saulnier, Nicole; Shearer, Jeffrey; Muthinti, Raja; Patlolla, Raghuveer; Huang, Huai

    2017-03-01

    Pattern transfer fidelity is always a major challenge for any lithography process and needs continuous improvement. Lithographic processes in semiconductor industry are primarily driven by optical imaging on photosensitive polymeric material (resists). Quality of pattern transfer can be assessed by quantifying multiple parameters such as, feature size uniformity (CD), placement, roughness, sidewall angles etc. Roughness in features primarily corresponds to variation of line edge or line width and has gained considerable significance, particularly due to shrinking feature sizes and variations of features in the same order. This has caused downstream processes (Etch (RIE), Chemical Mechanical Polish (CMP) etc.) to reconsider respective tolerance levels. A very important aspect of this work is relevance of roughness metrology from pattern formation at resist to subsequent processes, particularly electrical validity. A major drawback of current LER/LWR metric (sigma) is its lack of relevance across multiple downstream processes which effects material selection at various unit processes. In this work we present a comprehensive assessment of Line Edge and Line Width Roughness at multiple lithographic transfer processes. To simulate effect of roughness a pattern was designed with periodic jogs on the edges of lines with varying amplitudes and frequencies. There are numerous methodologies proposed to analyze roughness and in this work we apply them to programmed roughness structures to assess each technique's sensitivity. This work also aims to identify a relevant methodology to quantify roughness with relevance across downstream processes.

  7. Similarity law for Widom lines and coexistence lines

    Science.gov (United States)

    Banuti, D. T.; Raju, M.; Ihme, M.

    2017-05-01

    The coexistence line of a fluid separates liquid and gaseous states at subcritical pressures, ending at the critical point. Only recently, it became clear that the supercritical state space can likewise be divided into regions with liquidlike and gaslike properties, separated by an extension to the coexistence line. This crossover line is commonly referred to as the Widom line, and is characterized by large changes in density or enthalpy, manifesting as maxima in the thermodynamic response functions. Thus, a reliable representation of the coexistence line and the Widom line is important for sub- and supercritical applications that depend on an accurate prediction of fluid properties. While it is known for subcritical pressures that nondimensionalization with the respective species critical pressures pcr and temperatures Tcr only collapses coexistence line data for simple fluids, this approach is used for Widom lines of all fluids. However, we show here that the Widom line does not adhere to the corresponding states principle, but instead to the extended corresponding states principle. We resolve this problem in two steps. First, we propose a Widom line functional based on the Clapeyron equation and derive an analytical, species specific expression for the only parameter from the Soave-Redlich-Kwong equation of state. This parameter is a function of the acentric factor ω and compares well with experimental data. Second, we introduce the scaled reduced pressure pr* to replace the previously used reduced pressure pr=p /pcr . We show that pr* is a function of the acentric factor only and can thus be readily determined from fluid property tables. It collapses both subcritical coexistence line and supercritical Widom line data over a wide range of species with acentric factors ranging from -0.38 (helium) to 0.34 (water), including alkanes up to n-hexane. By using pr*, the extended corresponding states principle can be applied within corresponding states principle

  8. Spontaneous baroreflex sensitivity

    DEFF Research Database (Denmark)

    Barthel, Petra; Bauer, Axel; Müller, Alexander

    2012-01-01

    Low baroreflex sensitivity (BRS) indicates poor prognosis after acute myocardial infarction. Noninvasive BRS assessment is complicated by nonstationarities and noise in electrocardiogram and pressure signals. Phase-rectified signal averaging is a novel signal processing technology overcoming...

  9. MOVES sensitivity study

    Science.gov (United States)

    2012-01-01

    Purpose: : To determine ranking of important parameters and the overall sensitivity to values of variables in MOVES : To allow a greater understanding of the MOVES modeling process for users : Continued support by FHWA to transportation modeling comm...

  10. Cobalt sensitization and dermatitis

    DEFF Research Database (Denmark)

    Thyssen, Jacob P

    2012-01-01

    : This clinical review article presents clinical and scientific data on cobalt sensitization and dermatitis. It is concluded that cobalt despite being a strong sensitizer and a prevalent contact allergen to come up on patch testing should be regarded as a very complex metal to test with. Exposure...... data together with clinical data from metal workers heavily exposed to cobalt suggest that patch-test reactions are sometimes false positive and that patch testers should carefully evaluate their clinical relevance....

  11. Visceral sensitivity testing.

    Science.gov (United States)

    Andresen, Viola

    2009-01-01

    Visceral hypersensitivity is regarded as an important factor in the pathogenesis of functional gastrointestinal disorders. Assessment of visceral sensitivity has several important aims: increasing the understanding of normal and abnormal visceral sensory mechanisms and participating sensory pathways, serving as diagnostic tool to detect patients with abnormal visceral sensitivity, and evaluating therapeutic interventions directed towards modification of visceral sensitivity. Current stimulation modes in sensitivity tests include mechanical distension by barostat or tensostat, nutrient drink or water load, chemical stimulation, e.g. acid provocation or capsaicin ingestion, electrical, or thermal stimulation. Multimodal probes incorporating several stimulation modes in one device have recently been developed. Assessment of visceral sensation can be based on subjective responses of conscious perception or on objective parameters such as visceromotoric responses or central sensory processing patterns. All methods face the challenge that visceral sensitivity may be influenced by a wide spectrum of different factors, including the test techniques themselves, and improved, preferably non-invasive sensitivity tests with a greater standardisation and a broader applicability are still needed.

  12. 76 FR 51367 - China Shipping Container Lines Co., Ltd.; COSCO Container Lines Company Limited; Evergreen Line A...

    Science.gov (United States)

    2011-08-18

    ... China Shipping Container Lines Co., Ltd.; COSCO Container Lines Company Limited; Evergreen Line A Joint Service Agreement; Hanjin Shipping Co., Ltd.; Horizon Lines, LLC; Kawasaki Kisen Kaisha, Ltd.; Nippon... Container Lines Co., Ltd.; COSCO Container Lines Company Limited; Evergreen Line A Joint Service Agreement...

  13. Advanced Learners' Comprehension of Discourse Connectives: The Role of L1 Transfer across On-Line and Off-Line Tasks

    Science.gov (United States)

    Zufferey, Sandrine; Mak, Willem; Degand, Liesbeth; Sanders, Ted

    2015-01-01

    Discourse connectives are important indicators of textual coherence, and mastering them is an essential part of acquiring a language. In this article, we compare advanced learners' sensitivity to the meaning conveyed by connectives in an off-line grammaticality judgment task and an on-line reading experiment using eye-tracking. We also assess the…

  14. Design and fabrication process for artificial lateral line sensors

    NARCIS (Netherlands)

    Izadi, N.; Krijnen, Gijsbertus J.M.; Barth, Friedrich G.; Humphrey, Joseph A.C.; Srinivasan, Mandyam V.

    2011-01-01

    Biological sensory systems often display great performance, inspiring engineers to develop artificial counterparts. The lateral line system of fish has been widely studied by biologists for its crucial role in fish behaviour. Moreover, recently the robustness, sensitivity and consequently wide range

  15. On-line multidimensional separation systems for peptide analysis

    NARCIS (Netherlands)

    Stroink, T.

    2005-01-01

    Today, there is an increasing interest in selective and sensitive analysis of proteins and peptides with a relatively high speed. The first chapter of this thesis describes several strategies for the on-line multidimensional analysis of peptides and proteins in biological samples. This overview of

  16. Bipolar pulse forming line

    Science.gov (United States)

    Rhodes, Mark A.

    2008-10-21

    A bipolar pulse forming transmission line module for linear induction accelerators having first, second, third, fourth, and fifth planar conductors which form an interleaved stack with dielectric layers between the conductors. Each conductor has a first end, and a second end adjacent an acceleration axis. The first and second planar conductors are connected to each other at the second ends, the fourth and fifth planar conductors are connected to each other at the second ends, and the first and fifth planar conductors are connected to each other at the first ends via a shorting plate adjacent the first ends. The third planar conductor is electrically connectable to a high voltage source, and an internal switch functions to short a high voltage from the first end of the third planar conductor to the first end of the fourth planar conductor to produce a bipolar pulse at the acceleration axis with a zero net time integral. Improved access to the switch is enabled by an aperture through the shorting plate and the proximity of the aperture to the switch.

  17. Alternate Double Single Track Lines

    Energy Technology Data Exchange (ETDEWEB)

    Moraga Contreras, P.; Grande Andrade, Z.; Castillo Ron, E.

    2016-07-01

    The paper discusses the advantages and shortcomings of alternate double single track (ADST) lines with respect to double track lines for high speed lines. ADST lines consists of sequences of double and single track segments optimally selected in order to reduce the construction and maintenance costs of railway lines and to optimize the timetables used to satisfy a given demand. The single tracks are selected to coincide with expensive segments (tunnels and viaducts) and the double tracks are chosen to coincide with flat areas and only where they are necessary. At the same time, departure times are adjusted for trains to cross at the cheap double track segments. This alternative can be used for new lines and also for existing conventional lines where some new tracks are to be constructed to reduce travel time (increase speed). The ADST proposal is illustrated with some examples of both types (new lines and where conventional lines exist), including the Palencia-Santander, the Santiago-Valparaíso-Viña del Mar and the Dublin-Belfast lines, where very important reductions (90 %) are obtained, especially where a railway infrastructure already exist. (Author)

  18. On-line Dynamic Security Assessment in Power Systems

    DEFF Research Database (Denmark)

    Weckesser, Johannes Tilman Gabriel

    angular divergence of a group of generators can cause critical voltage sags at certain buses in the system. In this thesis assessment of such voltage sags using two types of sensitivities, which are derived from the algebraic network equations, is proposed. These sensitivities are derived after an in......-depth study of the mechanism causing the voltage sags. The first sensitivity type is called load voltage i/xii sensitivity and allows identifying which bus voltages are affected by a change in rotor angle of a particular generator. The second proposed type is called generator power sensitivity, which provides...... for early prediction of critical voltage sags is described. The method’s performance is compared to other prediction approaches. The results show that the proposed method succeeds in early, accurately and consistently predicting critically low voltage sags. An efficient on-line DSA not only identifies...

  19. Radiation-induced adaptive response in fish cell lines.

    Science.gov (United States)

    Ryan, Lorna A; Seymour, Colin B; O'Neill-Mehlenbacher, Alicia; Mothersill, Carmel E

    2008-04-01

    There is considerable interest at present in low-dose radiation effects in non-human species. In this study gamma radiation-induced adaptive response, a low-dose radiation effect, was examined in three fish cell lines, (CHSE-214 (Chinook salmon), RTG-2 (rainbow trout) and ZEB-2J (zebrafish)). Cell survival after exposure to direct radiation with or without a 0.1 Gy priming dose, was determined using the colony forming assay for each cell line. Additionally, the occurrence of a bystander effect was examined by measuring the effect of irradiated cell culture medium from the fish cell lines on unexposed reporter cells. A non-linear dose response was observed for all cell lines. ZEB-2J cells were very sensitive to low doses and a hyper-radiosensitive (HRS) response was observed for doses fish cell lines tested. Rather, it was found that pre-exposure of these cells to 0.1 Gy radiation sensitized the cells to subsequent high doses. In CHSE-214 cells, increased sensitivity to subsequent high doses of radiation was observed when the priming and challenge doses were separated by 4 h; however, this sensitizing effect was no longer present when the interval between doses was greater than 8 h. Additionally, a "protective" bystander response was observed in these cell lines; exposure to irradiated medium from fish cells caused increased cloning efficiency in unirradiated reporter cells. The data confirm previous conclusions for mammalian cells that the adaptive response and bystander effect are inversely correlated and contrary to expectations probably have different underlying mechanisms.

  20. Beyond equilibrium climate sensitivity

    Science.gov (United States)

    Knutti, Reto; Rugenstein, Maria A. A.; Hegerl, Gabriele C.

    2017-10-01

    Equilibrium climate sensitivity characterizes the Earth's long-term global temperature response to increased atmospheric CO2 concentration. It has reached almost iconic status as the single number that describes how severe climate change will be. The consensus on the 'likely' range for climate sensitivity of 1.5 °C to 4.5 °C today is the same as given by Jule Charney in 1979, but now it is based on quantitative evidence from across the climate system and throughout climate history. The quest to constrain climate sensitivity has revealed important insights into the timescales of the climate system response, natural variability and limitations in observations and climate models, but also concerns about the simple concepts underlying climate sensitivity and radiative forcing, which opens avenues to better understand and constrain the climate response to forcing. Estimates of the transient climate response are better constrained by observed warming and are more relevant for predicting warming over the next decades. Newer metrics relating global warming directly to the total emitted CO2 show that in order to keep warming to within 2 °C, future CO2 emissions have to remain strongly limited, irrespective of climate sensitivity being at the high or low end.

  1. Signal Transmission on Power Lines

    DEFF Research Database (Denmark)

    Dalby, Arne Brejning

    1997-01-01

    coupled transmission line circuits like power line circuits, is based on finding the eigenvalues and eigenvectors for the matrix ZY, where Z and Y are the impedance- and admittance matrices for the coupled lines in W/m and S/m. In this paper a flow-graph representation of the relations beween voltage......- and current-vectors is presented for a general coupled line circuit. This flow-graph can be used to find any transfer function on a multiple coupled lossy transmission line circuit. In some cases, however, it is difficult to find the eigenvalues correctly. A correct analysis can thus not be made......In the analysis of power-line networks over a large frequency span, the time-domain method used in programs like EMTP (Electromagnetic Time domain Program) can not be used. A more rigorous analysis method must be employed. The correct analysis method (assuming TEM-mode propagation) for multiple...

  2. Testing in Software Product Lines

    OpenAIRE

    Odia, Osaretin Edwin

    2007-01-01

    This thesis presents research aimed at investigating different activities involved in software product lines testing process and possible improvements towards achieving developing high quality software product lines at reduced cost and time. The research was performed using systematic review procedures of Kitchenham. The reviews carried out in this research covers several areas relating to software product lines testing. The reasons for performing a systematic review in this research are to; ...

  3. Sensitizing properties of proteins

    DEFF Research Database (Denmark)

    Poulsen, Lars K.; Ladics, Gregory S; McClain, Scott

    2014-01-01

    scientists from academia, government, and industry participated in the symposium. Experts provided overviews on known mechanisms by which proteins in food may cause sensitization, discussed experimental models to predict protein sensitizing potential, and explored whether such experimental techniques may......The scope of allergy risk is diverse considering the myriad ways in which protein allergenicity is affected by physiochemical characteristics of proteins. The complexity created by the matrices of foods and the variability of the human immune system add additional challenges to understanding...... Allergenicity Technical Committee of the International Life Sciences Institute's Health and Environmental Sciences Institute, featured presentations on current methods, test systems, research trends, and unanswered questions in the field of protein sensitization. A diverse group of over 70 interdisciplinary...

  4. Allergic sensitization: screening methods

    DEFF Research Database (Denmark)

    Ladics, Gregory S.; Fry, Jeremy; Goodman, Richard

    2014-01-01

    Experimental in silico, in vitro, and rodent models for screening and predicting protein sensitizing potential are discussed, including whether there is evidence of new sensitizations and allergies since the introduction of genetically modified crops in 1996, the importance of linear versus...... conformational epitopes, and protein families that become allergens. Some common challenges for predicting protein sensitization are addressed: (a) exposure routes; (b) frequency and dose of exposure; (c) dose-response relationships; (d) role of digestion, food processing, and the food matrix; (e) role...... potential of a novel protein. However, they would be extremely useful in the discovery and research phases of understanding the mechanisms of food allergy development, and may prove fruitful to provide information regarding potential allergenicity risk assessment of future products on a case by case basis...

  5. Sensitizing properties of proteins

    DEFF Research Database (Denmark)

    Poulsen, Lars K.; Ladics, Gregory S; McClain, Scott

    2014-01-01

    The scope of allergy risk is diverse considering the myriad ways in which protein allergenicity is affected by physiochemical characteristics of proteins. The complexity created by the matrices of foods and the variability of the human immune system add additional challenges to understanding...... the relationship between sensitization potential and allergy disease. To address these and other issues, an April 2012 international symposium was held in Prague, Czech Republic, to review and discuss the state-of-the-science of sensitizing properties of protein allergens. The symposium, organized by the Protein...... Allergenicity Technical Committee of the International Life Sciences Institute's Health and Environmental Sciences Institute, featured presentations on current methods, test systems, research trends, and unanswered questions in the field of protein sensitization. A diverse group of over 70 interdisciplinary...

  6. Developments in sensitivity theory

    Energy Technology Data Exchange (ETDEWEB)

    Cacuci, D.G.; Greenspan, E.; Marable, J.H.; Williams, M.L.

    1980-01-01

    A review of recent developments in sensitivity theory is presented with an emphasis on (a) extensions to new areas such as thermal hydraulics, reactor depletion, multi-constraint and extrema problems, and (b) recent mathematical refinements to and extensions of the basic theory. The diverse new areas of application are discussed from a unified theoretical viewpoint based on nonlinear functional analysis. Several new applications of sensitivity theory are presented for problems in constrained reactor physics calculations, irradiation experiment analysis, reactor burnup calculations, and transient thermal-hydraulic analysis. Future directions of the research are suggested.

  7. Metal Sensitivity in Children

    Directory of Open Access Journals (Sweden)

    S D Chaudhary

    1989-01-01

    Full Text Available One hundred children were patch tested with 5% nickel sulphate, 0.5% potassium dichromate, 5% cobalt chloride and pure petrolatum. Seventeen children (11 having eczematous and 6 having non-eczematous dermatoses showed positive patch test reactions to one or more metals, chromium (13%, nickel (8% and cobalt (1%. The incidence of metal sensitivity was highger (32.5% in the older children (8-14 years as compared to the younger children 7 years of age (2.66%. The female children had a higher incidence of sensitivity as compared to the male children.

  8. Rhode Island, Connecticut, New York, and New Jersey ESI: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for Rhode Island,...

  9. Cook Inlet and Kenai Peninsula, Alaska ESI: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for Cook Inlet...

  10. Guam and the Northern Mariana Islands ESI: HYDRO (Hydrography Lines and Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains vector lines and polygons representing coastal hydrography used in the creation of the Environmental Sensitivity Index (ESI) for Guam and the...

  11. Ultraviolet hypersensitivity of Cockayne syndrome lymphoblastoid lines - the effects of exogenous. beta. -nicotinamide adenine dinucleotide

    Energy Technology Data Exchange (ETDEWEB)

    Otsuka, Fujio; Kukita, Atsushi

    1986-12-01

    Four Cockayne Syndrome (CS) lymphoblastoid lines were tested for the lethal effects of UV radiation (254 nm) with or without addition of exogenous ..beta..-nicotinamide adenine dinucleotide (..beta..-NAD/sup +/) to their culture medium. Two of them exhibited a small but significantly increased resistance to UV radiation when ..beta..-NAD/sup +/ was added to the culture. However, their UV sensitivity after ..beta..-NAD+ addition was still much greater than that of normal control lines. Normal control lymphoblastoid lines and those from complementation group A and group C of xeroderma pigmentosum (XP) did not reveal any differences in post-UV sensitivity after the addition of exogenous ..beta..-NAD/sup +/. Thus the abnormal response to the lethal effects of UV radiation of CS lymphoblastoid lines could not be rectified by ..beta..-NAD/sup +/ addition. However, ..beta..-NAD/sup +/ does appear to play some partial role in reducing the high UV sensitivity of some CS lymphoblastoid lines.

  12. Self-Shielding Of Transmission Lines

    Energy Technology Data Exchange (ETDEWEB)

    Christodoulou, Christos [Univ. of New Mexico, Albuquerque, NM (United States)

    2017-03-01

    The use of shielding to contend with noise or harmful EMI/EMR energy is not a new concept. An inevitable trade that must be made for shielding is physical space and weight. Space was often not as much of a painful design trade in older larger systems as they are in today’s smaller systems. Today we are packing in an exponentially growing number of functionality within the same or smaller volumes. As systems become smaller and space within systems become more restricted, the implementation of shielding becomes more problematic. Often, space that was used to design a more mechanically robust component must be used for shielding. As the system gets smaller and space is at more of a premium, the trades starts to result in defects, designs with inadequate margin in other performance areas, and designs that are sensitive to manufacturing variability. With these challenges in mind, it would be ideal to maximize attenuation of harmful fields as they inevitably couple onto transmission lines without the use of traditional shielding. Dr. Tom Van Doren proposed a design concept for transmission lines to a class of engineers while visiting New Mexico. This design concept works by maximizing Electric field (E) and Magnetic Field (H) field containment between operating transmission lines to achieve what he called “Self-Shielding”. By making the geometric centroid of the outgoing current coincident with the return current, maximum field containment is achieved. The reciprocal should be true as well, resulting in greater attenuation of incident fields. Figure’s 1(a)-1(b) are examples of designs where the current centroids are coincident. Coax cables are good examples of transmission lines with co-located centroids but they demonstrate excellent field attenuation for other reasons and can’t be used to test this design concept. Figure 1(b) is a flex circuit design that demonstrate the implementation of self-shielding vs a standard conductor layout.

  13. Transmission Delay Modeling of Packet Communication over Digital Subscriber Line

    Directory of Open Access Journals (Sweden)

    Jiri Vodrazka

    2013-01-01

    Full Text Available Certain multimedia and voice services, such as VoIP, IPTV, etc., are significantly delay sensitive and their performance is influenced by the overall transmission delay and its variance. One of the most common solutions used in access networks are xDSL lines, especially ADSL2+ or VDSL2. Although these subscriber lines also use packet communication, there are several differences and mechanisms, which influence their resulting delay. Their delay characteristics are also dependent on the individual settings of each xDSL provider, therefore we decided to investigate this area for typical commercially available lines in Czech Republic. Based on the measured values and experiments with real ADSL2+ lines we also developed a potential modeling method, which is presented in this article as well. The parameters for packet jitter based on the generalized Pareto distribution were modeled.

  14. DISCOVERY OF POLARIZED LINE EMISSION IN SN 1006

    Energy Technology Data Exchange (ETDEWEB)

    Sparks, W. B.; Pringle, J. E.; Long, K. S.; Cracraft, M. [Space Telescope Science Institute, 3700 San Martin Drive, Baltimore, MD 21218 (United States); Carswell, R. F., E-mail: sparks@stsci.edu [Institute of Astronomy, University of Cambridge, Madingley Road, Cambridge, CB3 0HA (United Kingdom)

    2015-12-10

    Laming predicted that the narrow Balmer line core of the ∼3000 km s{sup −1} shock in the SN 1006 remnant would be significantly polarized due to electron and proton impact polarization. Here, based on deep spectrally resolved polarimetry obtained with the European Southern Observatory (ESO)’s Very Large Telescope (VLT), we report the discovery of polarized line emission with a polarization degree of 1.3% and position angle orthogonal to the SNR filament. Correcting for an unpolarized broad line component, the implied narrow line polarization is ≈2.0%, close to the predictions of Laming. The predicted polarization is primarily sensitive to shock velocity and post-shock temperature equilibration. By measuring polarization for the SN 1006 remnant, we validate and enable a new diagnostic that has important applications in a wide variety of astrophysical situations, such as shocks, intense radiation fields, high energy particle streams, and conductive interfaces.

  15. How sensitizing is chlorocresol?

    DEFF Research Database (Denmark)

    Andersen, Klaus Ejner; Hamann, K

    1984-01-01

    % in pet. showed 11 reactions among 1462 patients tested, but none were explainable and reproducible during re-tests and provocative use tests, indicating that the GPMT overestimated the sensitization potential. The results from guinea pig allergy tests cannot stand alone but have to be validated by other...

  16. Multiple chemical sensitivity

    DEFF Research Database (Denmark)

    Tran, Marie Thi Dao; Arendt-Nielsen, Lars; Kupers, Ron

    2013-01-01

    contributing to the clinical manifestations. PURPOSE: To use quantitative sensory testing (QST) to study central hyperexcitability and multiple aspects of central sensory processing in MCS patients without comorbid overlapping disorders and to compare the results with those among matched controls. METHODS: 15...... patients without comorbid, overlapping disorders, suggesting facilitated central sensitization in MCS....

  17. High-Sensitivity Spectrophotometry.

    Science.gov (United States)

    Harris, T. D.

    1982-01-01

    Selected high-sensitivity spectrophotometric methods are examined, and comparisons are made of their relative strengths and weaknesses and the circumstances for which each can best be applied. Methods include long path cells, noise reduction, laser intracavity absorption, thermocouple calorimetry, photoacoustic methods, and thermo-optical methods.…

  18. UNITEC SENS-IT

    Science.gov (United States)

    The UniTec Sens-It is a small gas-sensing device that can measure volatile organic compounds (VOCs). This sensor is used to measure VOCs in applications such as urban air quality, roadside pollution, and (solid waste) landfill monitoring. This operating procedure explains what yo...

  19. Sexuality Sensitive Schooling

    Science.gov (United States)

    McCaughtry, Nate; Dillon, Suzanna; Jones, Elizabeth; Smigell, Sara

    2005-01-01

    American schools, especially their physical education and sport programs, provide some of the most hostile social geographies in all of society for gay youth. With the aim of transforming schools toward more democratic and sexuality sensitive institutions, this paper reviews the literature on sexuality and education. In the review, three themes,…

  20. Multiple Chemical Sensitivity

    DEFF Research Database (Denmark)

    Junge, Anne Gram

    Et voksende antal mennesker i Danmark oplever at være overfølsomme over for dufte og kemikalier. Imidlertid er den tilskrevne diagnose Multiple Chemical Sensitivity (MCS) ikke medicinsk anerkendt i Danmark pga. mangel på organiske og patofysiologisk basis for symptomerne. Dette speciale bygger på...

  1. Dependence of NMR noise line shapes on tuning, matching, and transmission line properties.

    Science.gov (United States)

    Bendet-Taicher, Eli; Müller, Norbert; Jerschow, Alexej

    2014-02-01

    The tuning and matching conditions of rf circuits, as well as the properties of the transmission lines connecting these to the preamplifier, have direct consequences for NMR probe sensitivity and as for the optimum delivery of rf power to the sample. In addition, tuning/matching conditions influence radiation damping effects, which manifest themselves as fast signal flip-back and line broadening effects, and can lead to concentration-dependent frequency shifts. Previous studies have also shown that the appearance of spin-noise and absorbed circuit noise signals heavily depended on tuning settings. Consequently, all these phenomena are linked together. The mutual connections and interdependences of these effects are highlighted and reviewed here.

  2. Positive Predictive Value of the WHO Clinical and Immunologic Criteria to Predict Viral Load Failure among Adults on First, or Second-Line Antiretroviral Therapy in Kenya

    National Research Council Canada - National Science Library

    Waruru, Anthony; Muttai, Hellen; Ng'ang'a, Lucy; Ackers, Marta; Kim, Andrea; Miruka, Fredrick; Erick, Opiyo; Okonji, Julie; Ayuaya, Tolbert; Schwarcz, Sandra

    2016-01-01

    .... Studies have measured positive predictive value (PPV), negative predictive value, sensitivity and specificity of these criteria among patients receiving first-line ART but not specifically among those on second-line or subsequent regimens...

  3. Fractions, Number Lines, Third Graders

    Science.gov (United States)

    Cramer, Kathleen; Ahrendt, Sue; Monson, Debra; Wyberg, Terry; Colum, Karen

    2017-01-01

    The Common Core State Standards for Mathematics (CCSSM) (CCSSI 2010) outlines ambitious goals for fraction learning, starting in third grade, that include the use of the number line model. Understanding and constructing fractions on a number line are particularly complex tasks. The current work of the authors centers on ways to successfully…

  4. Marine line fish research programme

    CSIR Research Space (South Africa)

    SANCOR

    1979-04-01

    Full Text Available This report outlines the framework for a marine line fish programme under the aegis of the South African National Committee for Oceanographic Research (SANCOR). An attempt is made to assess the state of knowledge about South African marine line...

  5. relline: Relativistic line profiles calculation

    Science.gov (United States)

    Dauser, Thomas

    2015-05-01

    relline calculates relativistic line profiles; it is compatible with the common X-ray data analysis software XSPEC (ascl:9910.005) and ISIS (ascl:1302.002). The two basic forms are an additive line model (RELLINE) and a convolution model to calculate relativistic smearing (RELCONV).

  6. Drops, contact lines, and electrowetting

    NARCIS (Netherlands)

    't Mannetje, Dieter

    2013-01-01

    In this work, we study the behaviour of drops and contact lines under the influence of electric fields, and how these can answer fundamental and industrial questions. Our focus is on studying the varying balance of the electric field, hysteresis forces and inertia as the speed of a contact line

  7. A transmission line phase stabilizer

    Science.gov (United States)

    Lutes, G. F.

    1979-01-01

    To meet the phase stability requirements of certain experiments performed with the Deep Space Network, transmission lines carrying reference signals must be stabilized to reduce changes in their electrical length due to mechanical movement or changes in ambient temperature. A transmission line phase stabilizer being developed at JPL to perform this function is described.

  8. What was the Assembly Line?

    DEFF Research Database (Denmark)

    Nye, David

    2010-01-01

    The assembly line is still evolving a century after its invention, and it was not a distinct historical stage, nor was it part of an inevitable sequence that followed "Taylorism."......The assembly line is still evolving a century after its invention, and it was not a distinct historical stage, nor was it part of an inevitable sequence that followed "Taylorism."...

  9. US line-ups outperform UK line-ups.

    Science.gov (United States)

    Seale-Carlisle, Travis M; Mickes, Laura

    2016-09-01

    In the USA and the UK, many thousands of police suspects are identified by eyewitnesses every year. Unfortunately, many of those suspects are innocent, which becomes evident when they are exonerated by DNA testing, often after having been imprisoned for years. It is, therefore, imperative to use identification procedures that best enable eyewitnesses to discriminate innocent from guilty suspects. Although police investigators in both countries often administer line-up procedures, the details of how line-ups are presented are quite different and an important direct comparison has yet to be conducted. We investigated whether these two line-up procedures differ in terms of (i) discriminability (using receiver operating characteristic analysis) and (ii) reliability (using confidence-accuracy characteristic analysis). A total of 2249 participants watched a video of a crime and were later tested using either a six-person simultaneous photo line-up procedure (USA) or a nine-person sequential video line-up procedure (UK). US line-up procedure yielded significantly higher discriminability and significantly higher reliability. The results do not pinpoint the reason for the observed difference between the two procedures, but they do suggest that there is much room for improvement with the UK line-up.

  10. Fibrillary lines in overnight orthokeratology.

    Science.gov (United States)

    Lum, Edward; Swarbrick, Helen

    2007-07-01

    This case report describes the appearance of fibrillary lines in the anterior stroma of a 39-year-old Asian woman wearing overnight orthokeratology (OK) lenses. The fibrillary lines were fine, slightly curved and sub-epithelial, arranged in a band-like annulus in the corneal mid-periphery. The lines were not associated with epithelial staining, although a marked Fischer-Schweitzer corneal mosaic was noted after blinking. Fibrillary lines are a relatively common finding in normal and keratoconic corneas and have been reported previously accompanying OK lens wear. Their origin is unknown and epithelial neural remodelling, corneal biomechanical stress and abrupt corneal curvature changes have been suggested as contributing factors. The appearance of fibrillary lines in our OK patient had no adverse consequences on vision or ocular health, at least in the medium term.

  11. Formación on line On line learning

    Directory of Open Access Journals (Sweden)

    O. Grau-Perejoan

    2008-09-01

    Full Text Available La formación on line es una modalidad de enseñanza a distancia basada en las nuevas tecnologías. En este artículo se pretende hacer una introducción a base de describir a grandes rasgos sus características principales: asincronía, no presencialidad, comunicación escrita, función del profesor on line, así como los retos, los riesgos, las ventajas y los inconvenientes que plantea. Se exponen las diferencias entre la formación on line y la formación presencial, de manera que los docentes puedan adaptar de la mejor manera posible sus propuestas formativas a la modalidad on line. Se introduce el importantísimo papel de la planificación y de la fase de diseño y, finalmente, se repasan conceptos útiles para comprender mejor el mundo de la formación on line como son los conceptos entorno virtual de aprendizaje (EVA o Blended Learning (B-Learning.On line learning is a type of distance education based on new technologies. This article's aim is to introduce its main characteristics -asynchrony, non-presentiality, written communication, e-teacher role- as well as its challenges, risks, advantages and limitations. Differences between on line learning and face-to-face learning are presented in order to enable educational professionals to adapt their courses to the on line methodology. Planning and designing are introduced as key phases and, finally, useful concepts such as Virtual Learning Environment (VLE or Blended Learning (B-Learning are reviewed in order to achieve a better understanding of the on line learning field.

  12. Cyclotron Line Measurements with INTEGRAL

    Science.gov (United States)

    Pottschmidt, K.; Kreykenbohm, I.; Caballero, I.; Fritz, S.; Schoenherr, G.; Kretschmar, P.; Wilms, J.; McBride, V. A.; Suchy, S.; Rothschild, R. E.

    2008-01-01

    Due to its broadband energy coverage, INTEGRAL has made important contributions to observing and interpreting cyclotron lines, which are present in the 10-100 keV range of a sample of accreting pulsars. In these systems photons with energies fulfilling the resonance condition inelastically Compton scatter off electrons quantized in the accretion column above the neutron star's magnetic pole(s). This process gives rise to the broad, absorption-like lines or 'cyclotron resonant scattering features' (CRSF). The observed lines allow to directly measure the B-fields of these sources, resulting in values of a few times 1E12G. In this overview I will present recent highlights regarding CRSF observations as well as discuss current ideas and models for the physical conditions in the accretion column. Among the former are the stability of the spectrum of Vela X-1 during giant flares in 2003, the observation of three cyclotron lines during the 2004 outburst of V0332+53, the confirmation of the fundamental line at approximately 45 keV during a 2005 normal outburst of A0535-26, and the simultaneous detection of the two lines in the dipping source 4U 1907+09 (for which also a torque reversal was detected for the first time). Through these and other observations it has become increasingly apparent that two types of observations can potentially be used to constrain the accretion column geometry: the determination of energy ratios for multiple harmonic lines (only two sources with greater than 2 lines are known), was well as the evolution of the fundamental line centroid, which, for different sources, may or may not be correlated with flux. Furthermore, first steps have been taken away from the usual phenomenological description of the lines, towards a physical approach based on self-consistent CRSF modeling. Initial applications are presented.

  13. Proteomics of cancer cell lines resistant to microtubule-stabilizing agents

    DEFF Research Database (Denmark)

    Albrethsen, Jakob; Angeletti, Ruth H; Horwitz, Susan Band

    2014-01-01

    resistance to the class of MIAs known as microtubule-stabilizing agents (MSA). The human lung cancer cell line A549 was compared with two drug-resistant daughter cell lines, a taxol-resistant cell line (AT12) and an epothilone B (EpoB)-resistant cell line (EpoB40). The ovarian cancer cell line Hey......Despite the clinical success of microtubule-interacting agents (MIA), a significant challenge for oncologists is the inability to predict the response of individual patients with cancer to these drugs. In the present study, six cell lines were compared by 2D DIGE proteomics to investigate cellular...... increased in EpoB- and ixabepilone-resistant cells and its suppression caused an increase in drug sensitivity in both drug-sensitive and -resistant Hey cells. Furthermore, the growth medium from resistant Hey cells contained higher levels of galectin-1, suggesting that galectin-1 could play a role...

  14. Salt sensitivity correlates positively with insulin sensitivity in healthy volunteers

    NARCIS (Netherlands)

    ter Maaten, JC; Voordouw, JJ; Bakker, SJL; Gans, ROB

    Background The aim of the study was to assess the relationship between insulin sensitivity and salt sensitivity in healthy subjects who display a wide range of insulin sensitivity. As a secondary objective, we assessed the relationship between salt sensitivity and the other characteristics of the

  15. EVIDENCE FOR PHOTOIONIZATION-DRIVEN BROAD ABSORPTION LINE VARIABILITY

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Tinggui; Yang, Chenwei; Wang, Huiyuan [CAS Key Laboratory for Research in Galaxies and Cosmology, Department of Astronomy, University of Science and Technology of China, Hefei, Anhui 230026 (China); Ferland, Gary, E-mail: twang@ustc.edu.cn [Department of Physics and Astronomy, University of Kentucky, Lexington, KY 40506 (United States)

    2015-12-01

    We present a qualitative analysis of the variability of quasar broad absorption lines using the large multi-epoch spectroscopic data set of the Sloan Digital Sky Survey Data Release 10. We confirm that variations of absorption lines are highly coordinated among different components of the same ion or the same absorption component of different ions for C iv, Si iv, and N v. Furthermore, we show that the equivalent widths (EWs) of the lines decrease or increase statistically when the continuum brightens or dims. This is further supported by the synchronized variations of emission and absorption-line EWs when the well-established intrinsic Baldwin effect for emission lines is taken into account. We find that the emergence of an absorption component is usually accompanied by the dimming of the continuum while the disappearance of an absorption-line component is accompanied by the brightening of the continuum. This suggests that the emergence or disappearance of a C iv absorption component is only the extreme case, when the ionic column density is very sensitive to continuum variations or the continuum variability the amplitude is larger. These results support the idea that absorption-line variability is driven mainly by changes in the gas ionization in response to continuum variations, that the line-absorbing gas is highly ionized, and in some extreme cases, too highly ionized to be detected in UV absorption lines. Due to uncertainties in the spectroscopic flux calibration, we cannot quantify the fraction of quasars with asynchronized continuum and absorption-line variations.

  16. Bottlenecks in serial production lines: A system-theoretic approach

    Directory of Open Access Journals (Sweden)

    C.-T. Kuo

    1996-01-01

    Full Text Available In this work, a new definition of production systems bottlenecks is formulated and analyzed. Specifically, a machine is defined as the bottleneck if the sensitivity of the system's performance index to this machine's production rate in isolation is the largest. Although appealing from the systems point of view, this definition suffers a deficiency due to the fact that the sensitivities involved cannot be either measured on-line or efficiently calculated off-line. To avoid this, the paper develops a method based on indirect but real-time data. From this point of view, the main result of the work is as follows: The bottleneck machine in a serial production line can be identified by analyzing relationships between the so-called manufacturing blockage and manufacturing starvation of each machine. This leads to a simple rule for bottleneck identification. The rule requires neither the calculation of the production rate sensitivities nor the production rate itself. When the probabilities of manufacturing blockages and starvations are not available from on-line measurements, the paper presents their analytical estimates which, under certain conditions, can be used for bottleneck identification. Finally, a case study at an automotive component plant is described.

  17. The Cricket Cercal System Implements Delay-Line Processing

    Science.gov (United States)

    Mulder-Rosi, Jonas; Cummins, Graham I.

    2010-01-01

    The cercal sensory system of crickets mediates sensitivity to low-amplitude air currents. The sense organ for this system is a pair of antenna-like abdominal appendages called cerci, each of which is about 1 cm long in normal adult crickets. Although this system has been used extensively as a model system for studying the mechanisms underlying neural coding at the cellular and system levels, no previous studies have considered the functional significance of the physical dimensions of cerci. We show that the differential conduction characteristics of the receptor array in Acheta domesticus crickets are of substantial significance. All filiform sensory afferent axons we examined had the same propagation speeds to within a small variance, resulting in a significant and systematic differential propagation time for spikes from sensory receptors at different locations along the structure. Thus the sensory structures operate as delay lines. The delay-line structure supports neural computations in many of the projecting cercal interneurons (INs) that yield substantial differential sensitivity to the direction and velocity of naturalistic stimuli. Several INs show delay-line-derived sensitivities that are equivalent, in an engineering sense, to “notch filtering,” through which background noise is selectively eliminated by the delay-line-based processing. PMID:20107118

  18. Determinants of intrinsic radiosensitivity of mammalian cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Radford, I.R. [Peter MacCallum Cancer Institute, East Melbourne, VIC (Australia). Research Division

    1998-12-31

    Differences in the radiosensitivity of normal and cancerous cells could arise in various ways. Although there is no compelling data to support the view, the currently prevailing opinion is that differences in radiosensitivity are related to differences in some aspect of enzymatic DNA repair. A test of the importance of possible differences in enzymatic DNA repair in determining relative radiosensitivity would be to compare lethality in cells containing equivalent numbers of DNA lesions. Six cell lines were used in these studies: two Chinese hamster (CHO and V79) and a monkey (Vero) fibroblast-like line, a mouse melanoma line (B16-F1), and a rat (RUC-2) and a human (SQ-20B) carcinoma line. This group of cell lines displays a wide range of sensitivities to external beam low-LET radiation, ranging from the relatively radiosensitive B16-F1 and Vero lines through to the highly radioresistant RUC-2 line. However, it is important to note that none of the lines has a demonstrated defect in enzymatic DNA repair and that all appear to die by necrosis following a lethal radiation insult. Despite having significantly different radiosensitivities, CHO and V79 cells showed comparable responses to DNA-associated {sup 125}I-decays with D{sub o} values of around 65. More surprisingly, the radiosensitive B16-F1 line and the radioresistant RUC-2 line both had responses with D{sub o} values of around 133 {sup 125}I-decays. The factor of two difference between the D{sub o} values for these two pairs of cell lines is probably attributable to CHO and V79 cells being pseudo-diploid whereas B 16-F1 and RUC2 appear to have derived from tetraploid cells. The generality of the above result, for DNA lesions of different quality, was tested by comparing the sensitivities of CHO and V79 cells to DNA-associated {sup 3}H-decays. Again, consistent with the {sup 125}I-decay data, there was no significant difference in the D{sub o} values for these lines. Our {sup 3}H- and {sup 125}I-decay data are

  19. Teaching Braille Line Tracking Using Stimulus Fading

    Science.gov (United States)

    Scheithauer, Mindy C.; Tiger, Jeffrey H.

    2014-01-01

    Line tracking is a prerequisite skill for braille literacy that involves moving one's finger horizontally across a line of braille text and identifying when a line ends so the reader may reset his or her finger on the subsequent line. Current procedures for teaching line tracking are incomplete, because they focus on tracking lines with only…

  20. Atlas transmission line breakdown analysis

    CERN Document Server

    Nielsen, K E; Ballard, E O; Elizondo, J M; Gribble, R F; McCuistian, B T; Parsons, W M

    1999-01-01

    The Atlas facility will use 24 radially converging, vertically oriented and tapered, oil insulated, triplate transmission lines between the Marx generators and the central load region. Among the requirements of the transmission lines are low inductance and high reliability. The inter-conductor gap is nominally 2 cm and the lines taper from a height of 1.75 m at the Marx end to 0.32 m at the output end. The aluminum conductors, held together by 20 insulating spacers, are assembled and inserted as a unit into radial oil-filled steel tanks. The negative, high-voltage, center conductor is 2.54-cm thick and the outer ground conductors are 1.59-cm thick. All 24 triplate transmission lines connect to a transition section at near 1 m radius that couples the transmission lines to a disk/conical solid- dielectric-insulated power flow channel transmission line terminating at the load. Peak operating voltage on the lines can be as high as 240 kV with an effective stress time of 0.8 mu s. Testing of small sections of the ...