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Sample records for wild mink mustela

  1. Prevalence of giant kidney worm (Dioctophyma renale) in wild mink (Mustela vison) in Minnesota

    Science.gov (United States)

    Mech, L. David; Tracy, Shawn P.

    2001-01-01

    Of 138 wild mink (Mustela vison) from eastern Minnesota, 27% contained Dioctophyma renale, primarily in the right kidney. No significant difference between prevalence in adult male and immature male mink was found, nor between the prevalence in males versus female mink. Thirteen worms were found in one male mink, representing the highest documented infection intensity of a single wild mink.

  2. Mercury concentrations in wild mink (Mustela vison) and river otters (Lontra canadensis) collected from eastern and Atlantic Canada: Relationship to age and parasitism

    Energy Technology Data Exchange (ETDEWEB)

    Klenavic, Katherine [Environmental and Resource Studies, Trent University, 1600 West Bank Drive, Peterborough, ON K9J 7B8 (Canada); Champoux, Louise [Service Canadien de la Faune Environnement Canada, 1141 Route de l' Eglise, c.p. 10100, Sainte-Foy, QC G1V 4H5 (Canada)], E-mail: louise.champoux@ec.gc.ca; Mike, O' Brien [Furbearers and Upland Game, Department of Natural Resources, Kentville, NS B4N 4E5 (Canada)], E-mail: obrienms@gov.ns.ca; Daoust, Pierre-Y. [Department of Pathology and Microbiology, Atlantic Veterinary College, University of P.E.I., 550 University Avenue, Charlottetown, PEI C1A 4P3 (Canada)], E-mail: daoust@upei.ca; Evans, R. Douglas [Environmental and Resource Studies, Trent University, 1600 West Bank Drive, Peterborough, ON K9J 7B8 (Canada); Evans, Hayla E. [Environmental and Resource Studies, Trent University, 1600 West Bank Drive, Peterborough, ON K9J 7B8 (Canada)], E-mail: hevans@trentu.ca

    2008-11-15

    Total mercury (Hg) concentrations were measured in the fur, brain and liver of wild mink (Mustela vison) and river otters (Lontra canadensis) collected from eastern and Atlantic Canada. Total Hg concentrations in fur were strongly correlated with levels in the brain and liver. There was no difference in tissue concentrations between male and female mink; however, female otters had significantly higher fur, brain and liver Hg levels than males. Similarly, there was not a significant relationship between Hg concentration and age of mink, whereas in otters, Hg concentrations in all three tissues decreased significantly with age. In both species, only a very small percentage of the variability in Hg concentration was explained by age. After adjusting the data for site-to-site differences in Hg levels, Hg concentrations in the fur of mink infected by the parasite, Dioctophyma renale, were found to be significantly higher than Hg levels in uninfected mink. - Mercury (Hg) concentrations in liver, brain and fur are correlated in mink (Mustela vison) and otters (Lontra canadensis), allowing the use of fur as an indicator of internal tissue concentrations.

  3. Mercury concentrations in wild mink (Mustela vison) and river otters (Lontra canadensis) collected from eastern and Atlantic Canada: Relationship to age and parasitism

    International Nuclear Information System (INIS)

    Klenavic, Katherine; Champoux, Louise; Mike, O'Brien; Daoust, Pierre-Y.; Evans, R. Douglas; Evans, Hayla E.

    2008-01-01

    Total mercury (Hg) concentrations were measured in the fur, brain and liver of wild mink (Mustela vison) and river otters (Lontra canadensis) collected from eastern and Atlantic Canada. Total Hg concentrations in fur were strongly correlated with levels in the brain and liver. There was no difference in tissue concentrations between male and female mink; however, female otters had significantly higher fur, brain and liver Hg levels than males. Similarly, there was not a significant relationship between Hg concentration and age of mink, whereas in otters, Hg concentrations in all three tissues decreased significantly with age. In both species, only a very small percentage of the variability in Hg concentration was explained by age. After adjusting the data for site-to-site differences in Hg levels, Hg concentrations in the fur of mink infected by the parasite, Dioctophyma renale, were found to be significantly higher than Hg levels in uninfected mink. - Mercury (Hg) concentrations in liver, brain and fur are correlated in mink (Mustela vison) and otters (Lontra canadensis), allowing the use of fur as an indicator of internal tissue concentrations

  4. Mercury concentrations in wild mink (Mustela vison) and river otters (Lontra canadensis) collected from eastern and Atlantic Canada: relationship to age and parasitism.

    Science.gov (United States)

    Klenavic, Katherine; Champoux, Louise; Mike, O'Brien; Daoust, Pierre-Y; Evans, R Douglas; Evans, Hayla E

    2008-11-01

    Total mercury (Hg) concentrations were measured in the fur, brain and liver of wild mink (Mustela vison) and river otters (Lontra canadensis) collected from eastern and Atlantic Canada. Total Hg concentrations in fur were strongly correlated with levels in the brain and liver. There was no difference in tissue concentrations between male and female mink; however, female otters had significantly higher fur, brain and liver Hg levels than males. Similarly, there was not a significant relationship between Hg concentration and age of mink, whereas in otters, Hg concentrations in all three tissues decreased significantly with age. In both species, only a very small percentage of the variability in Hg concentration was explained by age. After adjusting the data for site-to-site differences in Hg levels, Hg concentrations in the fur of mink infected by the parasite, Dioctophyma renale, were found to be significantly higher than Hg levels in uninfected mink.

  5. Spatial and temporal differences in giant kidney worm, dictophyma renale, prevalence in Minnesota Mink, Mustela vison

    Science.gov (United States)

    Mech, L.D.

    2008-01-01

    Examination of 110 Mink (Mustela vison) carcasses from 1998 through 2007 indicated that the giant kidney worm, Dioctophyma renale, occurred in Pine and Kanabec Counties of eastern Minnesota with annual prevalences of 0-92%. Worm prevalence increased from 20% in 1999 to 92% in 2001 and decreased to 6% in 2005. During 2000 to 2007, no worms were found in Mink from Anoka and Chisago Counties (n = 54), and in 2000, none in 107 Mink from LeSeur, Freeborn, Redwood, Brown and Watonwan Counties. Changes in kidney worm prevalence were positively related to trapping success, considered an index of Mink density.

  6. Canine distemper of vaccine origin in European mink, Mustela lutreola--a case report.

    Science.gov (United States)

    Ek-Kommonen, C; Rudbäck, E; Anttila, M; Aho, M; Huovilainen, A

    2003-04-02

    Cases of canine distemper (CD) related to vaccination of exotic carnivores extend over three decades and have been described in at least nine different species. Our report describes a case of acute CD in a European mink, Mustela lutreola, vaccinated with live attenuated CD vaccine licensed for use in fur-farmed mink. The male mink died of an acute grey matter disease with an unusually long incubation period. A female vaccinated at the same time showed no obvious signs of illness. The diagnosis was confirmed by reverse transcriptase-polymerase chain reaction (RT-PCR) and by subsequent sequencing of the PCR products. The sequenced products of the virus isolated from the mink and of the vaccine batch showed 100% identity. This is the first report in which molecular methods were used to confirm that the disease was caused by the vaccine strain. Based on our findings, it is clearly evident that current CD vaccines cannot be safely used in exotic species.

  7. Naturally occurring Influenza A virus subtype H1N2 infection in a Midwest United States mink (Mustela vison) ranch.

    Science.gov (United States)

    Yoon, Kyoung-Jin; Schwartz, Kent; Sun, Dong; Zhang, Jianqiang; Hildebrandt, Hugh

    2012-03-01

    Influenza A virus (FLUAV) causes acute respiratory disease in humans and a variety of animal species. The virus tends to remain within the species of origin; nonetheless, naturally occurring cross-species transmission of FLUAV has been periodically documented. Multiple cross-species transmissions of FLUAV have been reported from companion animals and captive wild animals, neither of which is historically considered as natural hosts of FLUAV. In the fall of 2010, mink (Mustela vison) inhabiting a 15,000-head mink farm in the Midwest United States experienced persistent severe respiratory distress and nose and/or mouth bleeding. Mink losses averaged approximately 10 animals per day. Six dead mink at 6 months of age were submitted to the Iowa State University Veterinary Diagnostic Laboratory for diagnostic investigation. Gross and microscopic examinations revealed that all 6 mink had hemorrhagic bronchointerstitial pneumonia. Hemolytic Escherichia coli was isolated from lungs, probably accounting for hemorrhagic pneumonia. All animals tested negative for Canine distemper virus and Aleutian mink disease virus. Interestingly, FLUAV of H1N2 subtype, which contained the matrix gene of swine lineage, was detected in the lungs. Serological follow-up on mink that remained in the ranch until pelting also confirmed that the ranch had been exposed to FLUAV of H1 subtype (δ clade). The case study suggests that FLUAV should be included in the differential diagnosis when mink experience epidemics of respiratory disease. Since the source of FLUAV appeared to be uncooked turkey meat, feeding animals fully cooked ration should be considered as a preventive measure.

  8. Phytoestrogens alter the reproductive organ development in the mink (Mustela vison)

    International Nuclear Information System (INIS)

    Ryoekkynen, Ari; Nieminen, Petteri; Mustonen, Anne-Mari; Pyykoenen, Teija; Asikainen, Juha; Haenninen, Sari; Mononen, Jaakko; Kukkonen, Jussi V.K.

    2005-01-01

    The aim of the present study was to examine the reproductive effects of two perorally applied phytoestrogens, genistein (8 mg/kg/day) and β-sitosterol (50 mg/kg/day), on the mink (Mustela vison) at human dietary exposure levels. Parental generations were exposed over 9 months to these phytoestrogens and their offspring were exposed via gestation and lactation. Parents and their offspring were sampled 21 days after the birth of the kits. Sex hormone levels, sperm quality, organ weights, and development of the kits were examined. The exposed females were heavier than the control females at the 1st postnatal day (PND). The control kits were heavier than the exposed kits from the 1st to the 21st PND. Phytoestrogens did not affect the organ weights of the adult minks, but the relative testicular weight of the exposed kits was higher than in the control kits. The relative prostate weight was higher and the relative uterine weight lower in the β-sitosterol-exposed kits than in the control kits. Moreover, the plasma dihydrotestosterone levels were lower in the genistein-exposed male kits compared to the control male kits. This study could not explain the mechanisms behind these alterations. The results indicate that perinatal phytoestrogen exposures cause alterations in the weight of the reproductive organs of the mink kits

  9. Mink predation on brown trout in a Black Hills stream

    Science.gov (United States)

    Davis, Jacob L.; Wilhite, Jerry W.; Chipps, Steven R.

    2016-01-01

    In the early 2000’s, declines in the brown trout (Salmo trutta) fishery in Rapid Creek, South Dakota, caused concern for anglers and fisheries managers. We conducted a radio telemetry study in 2010 and 2011 to identify predation mortality associated with mink, using hatchery-reared (2010) or wild (2011) brown trout. Estimated predation rates by mink (Mustela vison) on radio-tagged brown trout were 30% for hatchery fish and 32% for wild fish. Size frequency analysis revealed that the size distribution of brown trout lost to predation was similar to that of other, radio-tagged brown trout. In both years, a higher proportion of predation mortality (83–92%) occurred during spring, consistent with seasonal fish consumption by mink. Predation by mink appeared to be a significant source of brown trout mortality in our study.

  10. A study of tobacco carcinogenesis XLVIII. Carcinogenicity of N'-nitrosonornicotine in mink (Mustela vison).

    Science.gov (United States)

    Koppang, N; Rivenson, A; Reith, A; Dahle, H K; Evensen, O; Hoffmann, D

    1992-11-01

    During tobacco processing and smoking, nicotine and nornicotine give rise to N'-nitrosonornicotine (NNN), a highly abundant, strong carcinogen. NNN is known to exert carcinogenic activity in mice, rats and hamsters. Major target organs for NNN carcinogenicity in the rat are the esophagus and the nasal mucosa, and in the Syrian golden hamster trachea and nasal mucosa. In comparison with the rat, the mink (Mustela vison) has a markedly expanded nasal mucosa. Therefore, we explored in this study whether the mink could serve as a non-rodent model for nasal carcinogenesis using NNN as the carcinogen. Twenty random-bred mink, beginning at the age of 3 weeks, received twice weekly s.c. injections of NNN, a total dose of 11.9 mM per animal over a 38 week period. All of the 19 mink at risk developed malignant tumors of both the respiratory and the olfactory region of the nose within 3.5 years. In most animals the malignant tumors, primarily esthesioneuroepithelioma, invaded the brain. Remarkably, NNN induced no other tumors in the mink. None of the control animals developed nasal tumors nor tumors at other sites during the 3.5 years of the assay. The historical data from the farm did not reveal any spontaneous occurrence of nasal tumors in mink at any age. This study supports the concept that NNN is a proven carcinogen for multiple species of mammals and that the mink can serve as a non-rodent, non-inbred animal model for nasal carcinogenesis, especially since NNN induces only tumors in the nasal cavity in this species and not at other sites, as it does in mice, rats and hamsters.

  11. Mortality and some biochemical changes in mink (Mustela vison) given sublethal doses of aflatoxin each day.

    Science.gov (United States)

    Chou, C C; Marth, E H; Shackelford, R M

    1976-10-01

    Two feeding trials were done to study the susceptibility of mink (Mustela vison) to multiple sublethal doses of aflatoxins. In the 1st trial, twenty 3-month-old male mink were divided equally among groups. Each mink in groups 1, 2, 3, and 4 was given a meatball daily that contained 15, 30, 45, or 0 mug of aflatoxins (B1:G1, 40:60), respectively. All mink in group 3 died between the 25th and the 30th days of the feeding trial. Each mink had ingested 1,035 to 1,480 mug of aflatoxins. Four of the mink in group 2 died almost as soon as did mink in group 3. Four mink in group 1 died between 40 and 59 days after the start of the feeding trial. Generally, a marked increase in plasma cholesterol and alkaline phosphatase activity appeared before mink died. The liver from animals that died of aflatoxicosis showed prominent pathologic changes which included hemorrhages and appearance of pink yellow spots. Histopathologic examination of liver from dead mink revealed fatty infiltration, bile duct proliferation, bile stasis, pseudotubular formation, congestion, and fibrosis. The feeding trial was repeated with 20 mink (8 males and 12 females) that were 1.5 to 2 years old. In this instance, 0, 20, 40, and 60 mug of aflatoxins were administered each day. All treated animals, except 1, were dead within 37 days after the experiment started. The survivor was given the lowest dosage of toxins and died after 52 days by which time 960 mug of aflatoxins were consumed. Plasma cholesterol content and alkaline phosphatase activity generally were similar to those observed in younger mink of the 1st feeding trial.

  12. Autumn-winter diet of three carnivores, European mink (Mustela lutreola, Eurasian otter (Lutra lutra and small-spotted genet (Genetta genetta, in northern Spain

    Directory of Open Access Journals (Sweden)

    Palazón, S.

    2008-12-01

    Full Text Available This study describes the autumn-winter diet of three carnivores (Mustela lutreola, Lutra lutra and Genetta genetta in northern Spain. Diet composition was analysed from 85 European mink, 156 otter and 564 spotted genet fecal samples The European mink diet was based on small mammals (relative frequency of occurrences 38.1%, fish (30.9% and birds (16.7%. Spotted genet consumed mainly small mammals, birds and fruits, whilst otter predated practically only fish (95%. Using Levins’ index, trophic-niche widths in European mink, small-spotted genet and Eurasian otter were 3.76, 3.77 and 1.10, respectively. The trophic niche overlap by Pianka index for autumn-winter was 0.77 for European mink vs. Small-spotted genet, and 0.60 for European mink vs. otter. The average size of brown trout taken by otter was larger than those consumed by European mink.

  13. DNA vaccines encoding proteins from wild-type and attenuated canine distemper virus protect equally well against wild-type virus challenge.

    Science.gov (United States)

    Nielsen, Line; Jensen, Trine Hammer; Kristensen, Birte; Jensen, Tove Dannemann; Karlskov-Mortensen, Peter; Lund, Morten; Aasted, Bent; Blixenkrone-Møller, Merete

    2012-10-01

    Immunity induced by DNA vaccines containing the hemagglutinin (H) and nucleoprotein (N) genes of wild-type and attenuated canine distemper virus (CDV) was investigated in mink (Mustela vison), a highly susceptible natural host of CDV. All DNA-immunized mink seroconverted, and significant levels of virus-neutralizing (VN) antibodies were present on the day of challenge with wild-type CDV. The DNA vaccines also primed the cell-mediated memory responses, as indicated by an early increase in the number of interferon-gamma (IFN-γ)-producing lymphocytes after challenge. Importantly, the wild-type and attenuated CDV DNA vaccines had a long-term protective effect against wild-type CDV challenge. The vaccine-induced immunity induced by the H and N genes from wild-type CDV and those from attenuated CDV was comparable. Because these two DNA vaccines were shown to protect equally well against wild-type virus challenge, it is suggested that the genetic/antigenic heterogeneity between vaccine strains and contemporary wild-type strains are unlikely to cause vaccine failure.

  14. [Population aspects of sexual dimorphism in guild of the Mustelidae: Mustela lutreola, Neovison vison, Mustela putorius, Martes martes as an example].

    Science.gov (United States)

    Korablev, M P; Korablev, N P; Korablev, P N

    2013-01-01

    Size sexual dimorphism was investigated on 695 skulls of four Mustelidae species. By extent of increasing of differences between sexes the species are placed in following order: European pine marten (Martes martes), European mink (Mustela lutreola), American mink (Neovison vison), and European polecat (Mustela putorius). Extent of the dimorphism characterizes ecological plasticity of the species and is population characteristic. It is shown that M. martes takes specific and relatively narrow ecological niche of forest ecosystems, entering into weak competitive relationships with smaller Mustelidae species. The level of sexual dimorphism of M. lutreola, N. vison and M. putorius reflects intensity of its interspecific relationships within study area. High level of sexual dimorphism of M. putorius is determined by further divergence of ecological niches of males and females, and also appears to be compensatory mechanism reducing consequences of hardened environmental requirements.

  15. Seroprevalence of Toxoplasma gondii in Danish farmed mink (Mustela vison S.)

    DEFF Research Database (Denmark)

    Henriksen, P; Dietz, H. H.; Uttenthal, Åse

    1994-01-01

    One hundred and ninety-five mink sera randomly selected from 17 Danish mink farms were evaluated for the presence of Toxoplasma gondii antibodies in the latex agglutination test. Six (3%) sera contained T. gondii antibodies in titres of 1:64 or more. The estimated 3% prevalence means that 300 000...... mink out of a total mink population of ten million might be infected with Toxoplasma gondii. This large number of possible sero-positive mink in Denmark indicates that there exists a potential risk of acquiring toxoplasmosis by pelting mink....

  16. Stress and stereotypic behaviour in mink (Mustela vison)

    DEFF Research Database (Denmark)

    Malmkvist, Jens; Jeppesen, Leif Lau; Palme, R.

    2011-01-01

    We examined whether female mink with low (LS) and high (HS) occurrence of stereotypic behaviour differ in their adrenocortical activity in baseline conditions or in response to immobilisation (Experiment 1), handling, adrenocorticotropic hormone (ACTH) challenge (Experiment 2) and excretion...... 4-20 h after the handling (P = 0.001). In Experiment 3 (n = 16), the excretion of infused (3)H-cortisol did not differ between LS and HS mink. Stereotypic behaviour is concurrent with higher baseline concentrations of FCM, which cannot be explained by a greater adrenocortical reactivity...... or a different excretion of the circulating cortisol. Instead, we conclude that mink with a high level of stereotypic behaviour have a greater perception of stress, or increased sensitivity to stressors at the pituitary level....

  17. Cadmium, mercury and selenium concentrations in mink (Mustela vison) from Yukon, Canada

    Energy Technology Data Exchange (ETDEWEB)

    Gamberg, Mary [Gamberg Consulting, Box 10460, Whitehorse, Yukon, Y1A 7A1 (Canada)]. E-mail: mary.gamberg@northwestel.net; Boila, Gail [Freshwater Institute, Fisheries and Oceans Canada, 501 University Crescent, Winnipeg, MB, R3T 2N6 (Canada); Stern, Gary [Freshwater Institute, Fisheries and Oceans Canada, 501 University Crescent, Winnipeg, MB, R3T 2N6 (Canada); Roach, Patrick [Department of Indian and Northern Affairs, Suite 300, 300 Main Street, Whitehorse, Yukon, Y1A 2B5 (Canada)

    2005-12-01

    Mercury (total and methyl), cadmium and selenium concentrations were measured in liver, kidney and brain tissue from mink trapped from the Yukon Territory from 2001-2002. None of these metals was found at levels of toxicological concern. Total mercury averaged 0.66, 0.92 and 0.22 {mu}g g{sup -1} in mink kidney, liver and brain tissue respectively, while methyl mercury averaged 0.77, 0.85 and 0.21 {mu}g g{sup -1} in the same tissues. Selenium averaged 2.07, 1.40 and 0.39 {mu}g g{sup -1} in mink kidney, liver and brain tissue, while cadmium was only measured in kidneys and averaged 0.22 {mu}g g{sup -1}. All element concentrations are presented on a wet weight basis. Concentrations of total mercury in all tissues were significantly higher in female than male mink, possibly reflecting proportionally greater food consumption by the smaller females. Total mercury concentrations were inversely related to the proportion of mercury present as methylmercury, and positively related to concentrations of selenium, consistent with increasing demethylation of methylmercury, and the formation of mercuric selenide as total concentrations of mercury increased. This relationship was seen most strongly in mink liver, less so in kidneys and not at all in brains where most of the mercury was maintained in the methyl form. There did not appear to be any geographical areas in which mink had obviously higher concentrations of mercury, and there was frequently a relatively large range of mercury levels found in mink from a given trapline. Mink diet may be a factor in this variation. Local environmental levels of cadmium were not reflected in cadmium concentrations in mink tissues. Mercury, cadmium and selenium do not appear to constitute environmental hazards to mink in the Yukon.

  18. Cadmium, mercury and selenium concentrations in mink (Mustela vison) from Yukon, Canada

    International Nuclear Information System (INIS)

    Gamberg, Mary; Boila, Gail; Stern, Gary; Roach, Patrick

    2005-01-01

    Mercury (total and methyl), cadmium and selenium concentrations were measured in liver, kidney and brain tissue from mink trapped from the Yukon Territory from 2001-2002. None of these metals was found at levels of toxicological concern. Total mercury averaged 0.66, 0.92 and 0.22 μg g -1 in mink kidney, liver and brain tissue respectively, while methyl mercury averaged 0.77, 0.85 and 0.21 μg g -1 in the same tissues. Selenium averaged 2.07, 1.40 and 0.39 μg g -1 in mink kidney, liver and brain tissue, while cadmium was only measured in kidneys and averaged 0.22 μg g -1 . All element concentrations are presented on a wet weight basis. Concentrations of total mercury in all tissues were significantly higher in female than male mink, possibly reflecting proportionally greater food consumption by the smaller females. Total mercury concentrations were inversely related to the proportion of mercury present as methylmercury, and positively related to concentrations of selenium, consistent with increasing demethylation of methylmercury, and the formation of mercuric selenide as total concentrations of mercury increased. This relationship was seen most strongly in mink liver, less so in kidneys and not at all in brains where most of the mercury was maintained in the methyl form. There did not appear to be any geographical areas in which mink had obviously higher concentrations of mercury, and there was frequently a relatively large range of mercury levels found in mink from a given trapline. Mink diet may be a factor in this variation. Local environmental levels of cadmium were not reflected in cadmium concentrations in mink tissues. Mercury, cadmium and selenium do not appear to constitute environmental hazards to mink in the Yukon

  19. Disease-associated prion protein in neural and lymphoid tissues of mink (Mustela vison) inoculated with transmissible mink encephalopathy.

    Science.gov (United States)

    Schneider, D A; Harrington, R D; Zhuang, D; Yan, H; Truscott, T C; Dassanayake, R P; O'Rourke, K I

    2012-11-01

    Transmissible spongiform encephalopathies (TSEs) are diagnosed by immunodetection of disease-associated prion protein (PrP(d)). The distribution of PrP(d) within the body varies with the time-course of infection and between species, during interspecies transmission, as well as with prion strain. Mink are susceptible to a form of TSE known as transmissible mink encephalopathy (TME), presumed to arise due to consumption of feed contaminated with a single prion strain of ruminant origin. After extended passage of TME isolates in hamsters, two strains emerge, HY and DY, each of which is associated with unique structural isoforms of PrP(TME) and of which only the HY strain is associated with accumulation of PrP(TME) in lymphoid tissues. Information on the structural nature and lymphoid accumulation of PrP(TME) in mink is limited. In this study, 13 mink were challenged by intracerebral inoculation using late passage TME inoculum, after which brain and lymphoid tissues were collected at preclinical and clinical time points. The distribution and molecular nature of PrP(TME) was investigated by techniques including blotting of paraffin wax-embedded tissue and epitope mapping by western blotting. PrP(TME) was detected readily in the brain and retropharyngeal lymph node during preclinical infection, with delayed progression of accumulation within other lymphoid tissues. For comparison, three mink were inoculated by the oral route and examined during clinical disease. Accumulation of PrP(TME) in these mink was greater and more widespread, including follicles of rectoanal mucosa-associated lymphoid tissue. Western blot analyses revealed that PrP(TME) accumulating in the brain of mink is structurally most similar to that accumulating in the brain of hamsters infected with the DY strain. Collectively, the results of extended passage in mink are consistent with the presence of only a single strain of TME, the DY strain, capable of inducing accumulation of PrP(TME) in the lymphoid

  20. Breath test measurements in combination with indirect calorimetry for estimation of 13C-leucine oxidation in mink (Mustela vison)

    DEFF Research Database (Denmark)

    Tauson, Anne-Helene; Ali, Abdalla; Kanska, Katarzyna

    2000-01-01

    Gas exchange measurements by means of indirect calorimetry can be used to calculate quantitative substrate oxidation. The results represents average net oxidation values (substrate disappearance rate), but they cannot describe the dynamics of the oxidation processes. Breath test measurements...... to feeding and fasting. Twelve 1-year-old male mink (Mustela vison) were measured in each five consecutive periods by means of indirect calorimetry and simultaneous breath test. In Periods 1, 3 and 5, each lasting 3 days, the animals were fed ad libitum and Periods 2 and 4 were fasting periods, each of 48 h....... In Periods 1 and 5 all animals were fed a diet with a high quality fish meal (FISH; n=12), while in Period 3 half of the animals received the FISH diet (n=6) and the other half a diet with soy protein concentrate (SOY; n=6) as main protein source. An intraperitoneal injection of 1-13C-leucine was given...

  1. Two avian H10 influenza A virus strains with different pathogenicity for mink (Mustela vison).

    Science.gov (United States)

    Englund, L; Hård af Segerstad, C

    1998-01-01

    We compared two strains of avian influenza A viruses of subtype H10 by exposing mink to aerosols of A/mink/Sweden/3,900/84 (H10N4) naturally pathogenic for mink, or A/chicken/Germany/N/49, (H10N7). Lesions in the respiratory tract during the first week after infection were studied and described. Both virus strains caused inflammatory reactions in the lungs and antibody production in exposed mink but only mink/84 virus was reisolated. The lesions caused by mink/84 virus were more severe with higher area density of pneumonia, lower daily weight gain, and more virus in the tissues detected by immunohistochemistry. The results indicate that mink/84 (H10N4), but not chicken/49 virus (H10N7), established multiple cycle replication in infected cells in the mink.

  2. The ventricles of the brain in the N. American mink (Mustela vison (Brisson, 1756))

    Energy Technology Data Exchange (ETDEWEB)

    Goscicka, D; Stankiewicz, W; Szpinda, M [Akademia Medyczna, Bydgoszcz (Poland)

    1994-12-31

    Using anatomical as well as radiographic and tomographic methods, sixty brains of the N. American mink were examined. It was found that the brain consists of four ventricles. Also, it was noted that the posterior horn was missing and that there was the olfactory recess present in the lateral ventricle, a large-size interthalamic connection present in the third ventricle, and a flat, necklace-like bottom in the fourth ventricle. Only recently, this ins and outs of the mink`s anatomical structure have begun to absorb anatomists. Apparently, it is related to the fact that furry animals, among them the mink, are being domesticated as if ``before our eyes``. For this reason and because of the easy access to material, examining of the brain ventricles in the mink was taken up. (author). 14 refs, 13 figs.

  3. Diurnal activity patterns of farm mink (Mustela vison) subjected to different feeding routines

    DEFF Research Database (Denmark)

    Hansen, Steffen W; Møller, Steen H

    2008-01-01

    synchronize their daily activity rhythm to the expected time of feeding. In addition, a high feeding motivation appears to be a precondition for circadian anticipatory activity in mink whereas anticipatory activity of mink fed ad libitum or close to ad libitum may be provoked by stimuli from the actual...

  4. Behaviour of mink kits and dams (Mustela vison) in the lactation period

    DEFF Research Database (Denmark)

    Brink, Anne-Line; Jeppesen, Leif Lau

    2005-01-01

    This study describes the development of the behaviour of mink kits and dams from the fourth to the eighth respectively seventh week after delivery. The study is based on scan observations of 72 mink dams and their kits at a conventional Danish mink farm. The kits started eating when they were about...... 30 d old. Drinking started almost 2 wk later. During these 2 wk there was a peak in the licking of saliva from the dam and of the inter-litter agonistic behaviour. A causal relationship between experienced thirst and agonistic behaviour is suggested. The kits' sucking declined to only 5% of the time...... budget in the seventh week and the stereotypy frequency of the dams increased to about 4% of the time budget. It is suggested that some dams are frustrated by the forced cohabitation with their nutritionally independent kits already in the seventh week and that this should be taken into account when...

  5. Verrucous endocarditis associated with Streptococcus bovis in mink (Mustela vison)

    DEFF Research Database (Denmark)

    Pedersen, Karl; Jørgensen, J.C.; Dietz, Hans-Henrik

    2003-01-01

    Between 1998 and 2001, mortalities due to verrucous endocarditis were experienced at several mink farms. Gram-positive cocci were isolated from the endocardium of all the animals examined but not always from other internal organs. Almost all the isolates were identified as Streptococcus bovis...

  6. Lesions in mink (Mustela vison) infected with giant kidney worm (Dioctophyma renale).

    Science.gov (United States)

    Mace, T F

    1976-01-01

    Adult Dioctophyma renale occupied the enlarged renal pelvis of the right kidney of naturally infected mink. Lesions in the kidney parenchyma consisted of connective tissue proliferation in the interstitial tissue, tubular atrophy and fibrosis, and periglomerular fibrosis. The luminal surface of the renal pelvis wall was formed of numerous papillae covered with transitional epithelium. The nematodes in the lumen were bathed in an albuminous fluid containing red blood cells, epithelial cells and D. renale eggs. The left (uninfected) kidney was 60% larger than the left kidney of normal mink.

  7. Humoral and cell-mediated immune responses in DNA immunized mink challenged with wild-type canine distemper virus.

    Science.gov (United States)

    Nielsen, Line; Søgaard, Mette; Karlskov-Mortensen, Peter; Jensen, Trine Hammer; Jensen, Tove Dannemann; Aasted, Bent; Blixenkrone-Møller, Merete

    2009-07-30

    The aim of the study was to investigate the different phases of the immune response after DNA immunization with the hemagglutinin and nucleoprotein genes from canine distemper virus (CDV). Although attenuated live CDV vaccines have effectively reduced the incidence of disease, canine distemper is still a problem worldwide. The broad host range of CDV creates a constant viral reservoir among wildlife animals. Our results demonstrated early humoral and cell-mediated immune responses (IFN-gamma) in DNA vaccinated mink compared to mock-vaccinated mink after challenge with a Danish wild-type CDV. The DNA vaccine-induced immunity protected the natural host against disease development.

  8. Running in a running wheel substitutes for stereotypies in mink (Mustela vison) but does it improve their welfare?

    DEFF Research Database (Denmark)

    Hansen, Steffen W; Damgaard, Birthe Marie

    2009-01-01

    This experiment investigated whether access to a running wheel affects the development of stereotypies during restricted feeding and whether selection for high or low levels of stereotypy affects the use of the running wheel. Sixty-two female mink kept in standard cages and selected for high or low...... levels of stereotypy were used. Thirty of these females had access to a running wheel whereas thirty-two female mink had no access to running wheels. The number of turns of the running wheel, behaviour, feed consumption, body weight and the concentration of plasma cortisol were measured during the winter...... period. Mink with access to a running wheel did not perform stereotypic behaviour and mink selected for a high level of stereotypies had more turns in the running wheel than mink selected for low levels of stereotypies. Mink with access to a running wheel used the running wheel for the same amount...

  9. Chemical and amino acid composition of colostrum and mature milk differ only slightly in mink (Mustela vison)

    DEFF Research Database (Denmark)

    Fink, Rikke; Rasmussen, Alice Neess; Tauson, Anne-Helene

    2007-01-01

    To determine differences in chemical composition between colostrum and mature milk in mink, milk samples were collected from 12 dams as close to the end of parturition as possible (n = 12), and at 24 h (n = 3), 48 h (n = 3) and 1 week (n = 12) postpartum. The milk samples were analysed for dry......, the differences between colostrum and mature milk were less pronounced in the mink than in many other species. Thus, colostrum seems to be of little importance in the mink in conferring passive immunity and hence for kit survival....

  10. Protein turnover in lactating mink (Mustela vison) is not affected by dietary protein supply

    DEFF Research Database (Denmark)

    Tauson, Anne-Helene; Fink, Rikke; Chwalibog, André

    2006-01-01

    The mink is a strict carnivore and may therefore serve as a model for the cat. Current recommendations for protein supply for lactating mink are based on production experiments with preweaning kit growth as a measure of dietary adequacy (1,2). Recently, nitrogen balance and substrate oxidation have...... in humans (7), growing pigs (8), and growing rats (9). In adult cats, both protein synthesis and breakdown were lower when feeding a low- than when feeding a high-protein diet [20 vs. 70% of metabolizable energy (ME)5 from protein] (10). The objectives of this study were therefore to develop a ¹5N...

  11. Alien Mink Predation and Colonisation Processes of Rodent Prey on Small Islands of the Baltic Sea: Does Prey Naivete Matter?

    International Nuclear Information System (INIS)

    Fey, K.; Korpimaki, E.; Banks, P.B.

    2010-01-01

    Colonisation, an important part of meta-population dynamics of fragmented populations, depends on both the dispersal ability and the ability to establish in the new habitat. Predation can hinder successful establishment of prey, and where the predation pressure comes from an alien predator, the effects on colonisation might be devastating. We studied the establishment of field voles (Microtus agrestis) inhabiting small islands of the archipelago of the Baltic Sea, SW Finland, under presence and absence of the alien American mink (Mustela vison). We translocated experienced voles from islands with mink, and inexperienced voles from islands from which mink had been removed, to other islands where mink was present or absent. By radio-tracking we studied survival, space and micro habitat use of voles within four weeks after translocation. Survival of voles on mink islands was significantly lower than on mink-free islands, but experienced voles did not survive better than inexperienced voles. Experienced voles were more often located in juniper habitats than inexperienced voles, but they appeared not to gain any survival benefit from altered micro habitat use. This study provides novel evidence, that alien mink predation inhibits establishment of colonising field voles and may thus ultimately induce extinction of voles from the outer archipelago.

  12. Mastitis in the lactating mink female (Mustela vison S.) and the development of "greasy kits"

    DEFF Research Database (Denmark)

    Clausen, T.N.; Dietz, Hans H.

    2000-01-01

    "Greasy kits" is the result ufa multifactorial disease complex with few known definitive aetiological factors. Mastitis has been hypothesized as a triggering factor although classical clinical signs of mastitis (rubor, tumor, dolor, calor) are rarely seen in lactating Danish mink Females. In this......"Greasy kits" is the result ufa multifactorial disease complex with few known definitive aetiological factors. Mastitis has been hypothesized as a triggering factor although classical clinical signs of mastitis (rubor, tumor, dolor, calor) are rarely seen in lactating Danish mink Females...

  13. The concept of behavioural needs in contemporary fur science

    DEFF Research Database (Denmark)

    Kornum, A.L.; Röcklinsberg, H.; Gjerris, Mickey

    2017-01-01

    show that mink place high value on swimming water, whereas other studies indicate the opposite, which has led scientists to question whether this preference constitutes a genuine behavioural need. In this paper, we take a methodological turn and discuss whether the oft-used concept of behavioural needs......This paper discusses the ethical implications of applying the concept of behavioural needs to captive animals. This is done on the basis of analysing the scientific literature on farmed mink and their possible need for swimming. In the wild, American mink (Mustela vison) are semi-aquatic predators...

  14. Hepatitis E Virus Variant in Farmed Mink, Denmark

    DEFF Research Database (Denmark)

    Krog, Jesper Schak; Breum, Solvej Østergaard; Jensen, Trine Hammer

    2013-01-01

    Hepatitis E virus (HEV) is a zoonotic virus for which pigs are the primary animal reservoir. To investigate whether HEV occurs in mink in Denmark, we screened feces and tissues from domestic and wild mink. Our finding of a novel HEV variant supports previous findings of HEV variants in a variety...

  15. [Evaluation of the immune response after vaccination against distemper at a mink (Mustela vison) farm in Argentina].

    Science.gov (United States)

    Jar, A M; Ramayo, L G; Stempler, A; Goldman, L H; Mundo, S L

    2010-01-01

    Distemper virus causes a disease affecting minks with respiratory, gastrointestinal, neurological and skin symptoms and showing high morbidity and mortality, mainly among puppies. It is controlled through immunization, using vaccines that are supplied for mink use. The aim of this work was to determine the seroneutralization titer against the distemper virus at a mink farm in Argentina. The antibody kinetics obtained after vaccination in 27 adult animals, as well as the duration of colostrum-transferred antibodies in 10 puppies were determined. All vaccinated adult minks showed protective titers up to at least 3 months after vaccination, and 37.5% significantly reduced their antibody levels, 12 months after vaccination. Only 20% of the puppies showed protective levels of colostrum-transferred antibodies at the age of 7 weeks, while non-detectable levels of antibodies were found when puppies reached 11 weeks old. Vaccination performed in these puppies at the age of 13 weeks, elicited protective seroneutralization titers. These results show that vaccination induces a satisfactory humoral immune response in our environment, and support the convenience of vaccinating dams annually before the beginning of the breeding season. The vaccination plan in puppies is also discussed.

  16. Water baths for farmed mink: intra-individual consistency and inter-individual variation in swimming behaviour, and effects on stereotyped behaviour

    Directory of Open Access Journals (Sweden)

    J. MONONEN

    2008-12-01

    Full Text Available Swimming behaviour and effects of water baths on stereotyped behaviour in farmed mink (Mustela vison were studied in three experiments. The singly-housed mink had access from their home cages to extra cages with 20.5 litre water baths. Two short-term experiments aimed to investigate how quickly adult and juvenile mink start using and how consistently they use water baths over 10 days, and whether the extent of the use correlates between dams and their females kits. A four-month experiment was designed to compare the development of stereotyped behaviour in juvenile mink housed with and without swimming opportunity. The behavioural analyses were based on several 24-hour video recordings carried out in all three experiments. There were obvious inter-individual differences and intra-individual consistency in swimming frequency and time. Farmed mink’s motivation to swim can be assessed in short-term experiments, and measurement of water losses from the swimming baths and use of instantaneous sampling with 10 min sampling intervals provide quite reliable measures of the amount of swimming. The bath use of the juveniles correlated with that of their dams, indicating that an individual mink’s eagerness to swim may have a genetic component. The lower amount of stereotyped behaviour in mink housed with water baths indicates that long-term access to baths may alleviate frustration in singly-housed juvenile farmed mink.;

  17. Production of mink enteritis parvovirus empty capsids by expression in a baculovirus vector system: a recombinant vaccine for mink enteritis parvovirus in mink

    DEFF Research Database (Denmark)

    Christensen, J; Alexandersen, Søren; Bloch, B.

    1994-01-01

    The VP-2 gene of mink enteritis parvovirus (MEV) was amplified by the polymerase chain reaction using MEV DNA isolated from the faeces of a naturally infected mink. Subsequently the VP-2 gene was cloned into a baculovirus expression vector. Recombinant baculo-viruses were isolated and the MEV VP-2...... protein was able to form parvovirus-like particles, which had haemagglutinating properties comparable with the wild-type MEV. The cloned VP-2 gene was sequenced and only five nucleotide differences were found after alignment with the known sequences of the MEV type 1 and type 2 isolates. Surprisingly...

  18. Interactions between retinol, α-tocopherol and cholecalciferol need consideration in diets for farmed mink (Mustela vison)

    DEFF Research Database (Denmark)

    Hymøller, Lone; Clausen, Tove N.; Jensen, Søren Krogh

    2016-01-01

    A sufficient but balanced vitamin supplementation is a prerequisite for a satisfactory growth pattern and an effective immune system in mink and all other species. The fat-soluble vitamins are very sensitive to over- or under-supply because they interact with each other with respect to dose...... had higher concentrations of fat-soluble vitamins in plasma than male mink....

  19. Serogroups and antimicrobial susceptibility among Escherichia coli isolated from farmed mink (Mustela vison Schreiber) in Denmark

    DEFF Research Database (Denmark)

    Vulfson, L.; Pedersen, Karl; Chriel, M.

    2001-01-01

    Escherichia coli is commonly found in outbreaks of diarrhoea in mink during the production season although its role as a primary causal organism remains unclear. The present study was undertaken to determine the serogroups and antimicrobial susceptibility of E. coli isolates from healthy and diar......Escherichia coli is commonly found in outbreaks of diarrhoea in mink during the production season although its role as a primary causal organism remains unclear. The present study was undertaken to determine the serogroups and antimicrobial susceptibility of E. coli isolates from healthy...... diseased. All isolates were serotyped and MICs were determined for nine antimicrobial compounds. Non-haemolytic isolates numbered 147, whereas 63 were haemolytic. Both haemolytic and non-haemolytic isolates were isolated from both healthy and diseased animals. A wide range of serogroups was detected...... among the six mink farms, for tetracycline (0-16.4%. average 21.9), ampicillin (2.9-50.0%. average 23.3), spectinomycin (8.0-35.7%. average 21.9), sulfamethoxazole (8.6-57.7%. average 30.0) and trimethoprim (0-35.7%. average 9.5). Resistance to tetracycline was statistically more prevalent among...

  20. Utilization of milk amino acids for body gain in suckling mink (Mustela vison) kits

    DEFF Research Database (Denmark)

    Tauson, Anne-Helene; Fink, Rikke; Hansen, Niels E

    2005-01-01

    The efficiency of utilization of milk amino acids for body gain in suckling mink kits from small (n = 3), medium (n = 6) and large litters (n = 9) was investigated by using 36 mink dams and their litters for measurements during lactation weeks 1 through 4. Measurements on each dam and litter were...... performed once, hence three dams per litter size each week (n = 9). Individual milk intake of kits was determined, milk samples were collected and kits were killed for determination of amino acid composition. The most abundant amino acids in milk were glutamate, leucine and aspartate making up about 40......% of total amino acids. Branched chained amino acids made up slightly more than 20% and sulphur containing amino acids less than 5% of total milk amino acids. In kit bodies the sum of glutamate, aspartate and leucine made up about 32% of amino acids, branched chain amino acids about 16% and sulphur...

  1. Acid-base status and cardiovascular function in mink (Mustela vison) anaesthetized with ketamine/midazolam.

    Science.gov (United States)

    Wamberg, S; Svendsen, P; Johansen, B

    1996-01-01

    Heart rate, arterial blood pressure and blood acid-base status were determined in 18 adult female mink (mean (+/- SEM) body weight 1052 +/- 34 g) during long-term anaesthesia with either controlled ventilation (n=12) or spontaneous respiration (n=6). Surgical anaesthesia was induced by intramuscular injection of ketamine hydrochloride (Ketaminol Vet, 40.0 +/- 1.7 mg/kg) and midazolam hydrochloride (Dormicum 2.8 +/- 0.1 mg/kg) and maintained for at least 5 h by continuous intravenous infusion of this drug combination in 0.9% saline. For all animals, the mean rates of infusion of ketamine and midazolam were 48.4 +/- 1.6 and 1.61 +/- 0.12 mg/h, respectively. Following continuous infusion of the anaesthetics in isotonic saline, at a rate of 20 ml/h, a moderate 'dilution acidosis' developed, which could be corrected by replacement of part of the saline with sodium bicarbonate to a final concentration of approximately 25 mmol NaHCO3 per litre. However, when the animals were allowed to breathe spontaneously, an increase in heart rate and a combined respiratory and metabolic acidosis occurred, due to severe respiratory depression. Apart from these effects and a few cases of increased salivation, no adverse effects over time were observed on the arterial blood acid-base status and cardiovascular function of the animals during ketamine/midazolam anaesthesia. It is concluded that the procedure described for long-term anaesthesia in mink is convenient and safe for acute physiological experiments in this species, provided normal body temperature and pulmonary gas exchange is sufficiently maintained. Thus, the need for an adequately controlled artificial ventilation is strongly emphasized. Finally, a proposal for the composition of an intravenous solution, containing ketamine and midazolam hydrochloride, and sodium bicarbonate in saline, suitable for long-term anaesthesia in adult mink is presented.

  2. Identifying QTL for fur quality traits in mink (Neovison vison)

    DEFF Research Database (Denmark)

    Thirstrup, Janne Pia; Anistoroaei, Razvan Marian; Guldbrandtsen, Bernt

    2012-01-01

    Mapping of quantitative trait loci (QTL) affecting fur quality traits (guard hair length, guard hair thikness, and density of woll) was performed in a 3-generation population (F2-design). In the parental generation, Nordic wild mink were crossed reciprocally with American short nap mink. Twenty o...

  3. Nitrogen and energy balance in growing mink (Mustela vison) fed different levels of bacterial protein meal produced with natural gas

    DEFF Research Database (Denmark)

    Hellwing, Anne Louise Frydendahl; Tauson, Anne-Helene; Ahlstrøm, Øystein

    2005-01-01

    The objective of this study was to estimate the effect of increasing the dietary content of bacterial protein meal (BPM) on energy and protein metabolism in growing mink kits. Sixteen male mink kits of the standard brown genotype were randomly fed one of four diets: A control (Diet III) and 60% (...

  4. Monitoring chronic infection with a field strain of Aleutian mink disease virus

    DEFF Research Database (Denmark)

    Jensen, Trine Hammer; Hammer, Anne Sofie; Chriél, Mariann

    2014-01-01

    to detect viral DNA in full blood, organs, faeces and oro-nasal swabs weekly for the first 8 weeks and then biweekly for another 16 weeks after AMDV challenge inoculation of wild type mink. The mink (n=29) was infected and seroconverted 2–3 weeks after AMDV inoculation and AMDV antibodies persisted during...... the maximum experimental period of 24 weeks. Viraemia and faecal excretion of viral DNA was detected in the mink (n=29) at various and intermittent time intervals. Excretion of viral DNA in oro-nasal swabs was detected for 1–8 weeks in 21 mink. This highlights the risk of transmitting AMDV between infected...

  5. Selection against stereotyped behaviour may have contradictory consequences for the welfare of farm mink (Mustela vison)

    DEFF Research Database (Denmark)

    Svendsen, Pernille Maj; Jeppesen, Leif Lau; Malmkvist, Jens

    2007-01-01

    The present study aimed to examine if divergent selection for stereotypic behaviour in mink influences the welfare of the animals. Two breeding lines were used, a high stereotyping line (HSL, N = 139) and a low stereotyping line (LSL, N = 132). Their welfare was assessed on the basis...... of adrenocortical activity (faecal cortisol metabolites, FCM), confident versus fearful temperament, growth and reproduction. The results showed that the LSL performed less stereotypic behaviour and were less active than the HSL. Furthermore, the results demonstrated that: (1) the LSL had lower concentrations......) there was no failure to grow in any line, but on average the body weight in the LSL was higher than that in the HSL. The correlation between stereotypy and confidence in the present study supports earlier findings showing a higher proportion of fearful animals in groups of low stereotyping mink. So, although...

  6. Genetic characterization of canine distemper virus involved in outbreaks in farmed mink in Denmark 2012

    DEFF Research Database (Denmark)

    Trebbien, Ramona; Struve, T.; Hjulsager, Charlotte Kristiane

    Danish farmed mink herds experienced a large outbreak of canine distemper virus in 2012. Full-length sequence analysis (1824 nucleotides) of the variable hemagglutinin (H) gene were performed on 27 viruses collected from mink and on 7 viruses collected from wild foxes. Results of the study showed...... with other European canine distemper viruses and showed the highest level of similarity (99.3 - 99.6 %) to viruses isolated from wild foxes in Germany. The fox should therefore be considered as an important wild life reservoir of canine distemper virus and may also contribute to the transmission of the virus...

  7. The urinary metabolome in female mink (Mustela neovison) shows distinct changes in protein and lipid metabolism during the transition from diapause to implantation

    DEFF Research Database (Denmark)

    Hedemann, Mette Skou

    2017-01-01

    Introduction The mink exhibit an obligatory diapause. The metabolic changes during the transition from diapause to implantation and established pregnancy are currently unknown. Objectives The study aimed to characterize changes in the urinary metabolome in mink during the period from mating...... to early gestation and to identify the metabolites involved. Methods Urine samples were collected from 56 female mink on March 24, April 8, and April 15, covering the period from mating to early pregnancy. The urine samples were subjected to non-targeted LC-MS metabolomics. Processed data were evaluated...

  8. Coronavirus infection in mink (Mustela vison). Serological evidence of infection with a coronavirus related to transmissible gastroenteritis virus and porcine epidemic diarrhea virus

    DEFF Research Database (Denmark)

    Have, P; Moving, V; Svansson, V

    1992-01-01

    Antibodies to a transmissible gastroenteritis virus (TGEV)-related coronavirus have been demonstrated in mink sera by indirect immunofluorescence, peroxidase-linked antibody assays and immunoblotting. This is the first serological evidence of a specific coronavirus infection in mink. The putative...

  9. Molecular and morphometric study of metacercariae and adults of Pseudamphistomum truncatum (Opisthorchiidae) from roach (Rutilus rutilus) and wild American mink (Mustela vison)

    DEFF Research Database (Denmark)

    Skov, Jakob; Kania, Per Walter; Jørgensen, Thomas Rohde

    2008-01-01

    Den digene ikte Pseudamphistomum forekommer som metacercarie i skaller fra Furesøen og som adult i mink fra området. Dette er belyst ved morfometriske og molekylære metoder. Fundet er væsentligt, idet parasitten er zoonotisk og kan inficere mennesker, der indtager utilstrækkeligt varmebehandlet f...

  10. Wildlife Reservoirs of Canine Distemper Virus Resulted in a Major Outbreak in Danish Farmed Mink (Neovison vison)

    Science.gov (United States)

    Trebbien, Ramona; Chriel, Mariann; Struve, Tina; Hjulsager, Charlotte Kristiane; Larsen, Gitte; Larsen, Lars Erik

    2014-01-01

    A major outbreak of canine distemper virus (CDV) in Danish farmed mink (Neovison vison) started in the late summer period of 2012. At the same time, a high number of diseased and dead wildlife species such as foxes, raccoon dogs, and ferrets were observed. To track the origin of the outbreak virus full-length sequencing of the receptor binding surface protein hemagglutinin (H) was performed on 26 CDV's collected from mink and 10 CDV's collected from wildlife species. Subsequent phylogenetic analyses showed that the virus circulating in the mink farms and wildlife were highly identical with an identity at the nucleotide level of 99.45% to 100%. The sequences could be grouped by single nucleotide polymorphisms according to geographical distribution of mink farms and wildlife. The signaling lymphocytic activation molecule (SLAM) receptor binding region in most viruses from both mink and wildlife contained G at position 530 and Y at position 549; however, three mink viruses had an Y549H substitution. The outbreak viruses clustered phylogenetically in the European lineage and were highly identical to wildlife viruses from Germany and Hungary (99.29% – 99.62%). The study furthermore revealed that fleas (Ceratophyllus sciurorum) contained CDV and that vertical transmission of CDV occurred in a wild ferret. The study provides evidence that wildlife species, such as foxes, play an important role in the transmission of CDV to farmed mink and that the virus may be maintained in the wild animal reservoir between outbreaks. PMID:24454897

  11. Wildlife reservoirs of canine distemper virus resulted in a major outbreak in Danish farmed mink (Neovison vison.

    Directory of Open Access Journals (Sweden)

    Ramona Trebbien

    Full Text Available A major outbreak of canine distemper virus (CDV in Danish farmed mink (Neovison vison started in the late summer period of 2012. At the same time, a high number of diseased and dead wildlife species such as foxes, raccoon dogs, and ferrets were observed. To track the origin of the outbreak virus full-length sequencing of the receptor binding surface protein hemagglutinin (H was performed on 26 CDV's collected from mink and 10 CDV's collected from wildlife species. Subsequent phylogenetic analyses showed that the virus circulating in the mink farms and wildlife were highly identical with an identity at the nucleotide level of 99.45% to 100%. The sequences could be grouped by single nucleotide polymorphisms according to geographical distribution of mink farms and wildlife. The signaling lymphocytic activation molecule (SLAM receptor binding region in most viruses from both mink and wildlife contained G at position 530 and Y at position 549; however, three mink viruses had an Y549H substitution. The outbreak viruses clustered phylogenetically in the European lineage and were highly identical to wildlife viruses from Germany and Hungary (99.29% - 99.62%. The study furthermore revealed that fleas (Ceratophyllus sciurorum contained CDV and that vertical transmission of CDV occurred in a wild ferret. The study provides evidence that wildlife species, such as foxes, play an important role in the transmission of CDV to farmed mink and that the virus may be maintained in the wild animal reservoir between outbreaks.

  12. Endogenous zinc excretion in relation to various levels of dietary zinc intake in the mink (Mustela vison)

    International Nuclear Information System (INIS)

    Mejborn, H.

    1990-01-01

    Endogenous zinc excretion was studied in adult male mink fed experimental diets for 73 d, including a collection period from d 69 to 73. Dietary zinc levels were 2.8, 26 or 121 mg/kg wet weight. In accordance with the results of a methodological study, also reported here, the animals had an intramuscular injection of 65ZnCl2 12 d before the start of the collection period. Total fecal (endogenous + unabsorbed) zinc excretion for d 69-73 in the three groups was 2.3, 20.4 and 91.0 mg. The endogenous zinc excretion was 1.3, 2.0 and 6.4 mg, corresponding to 80.8, 10.6 and 6.4% of the zinc intake. Thus, the endogenous excretion was mainly important for the zinc homeostasis at low zinc intake, whereas at high intake the homeostasis was regulated via absorption from the digestive tract. The overall conclusion of the experiment was that mink are comparable to other species (including man) in regard to mechanisms controlling zinc homeostasis

  13. Asthma among mink workers

    DEFF Research Database (Denmark)

    Grøntved, Berit; Carstensen, Ole; Petersen, Rolf

    2014-01-01

    We report two cases of asthma among mink workers. The first case is about a mink farmer who had asthma that was difficult to treat. In the medical history there was no clear relation to work, and no conclusive work relation with peak flow monitoring. He had a positive histamine release test to mink...... urine. The second case is about a mink farm worker, who had an asthma attack when handling mink furs. Peak flow monitoring showed a clear relation to this work, but there were no signs of allergy. We conclude that these two cases suggest an increased risk of asthma among mink workers....

  14. Aleutian Mink Disease Virus in Free-Ranging Mink from Sweden

    DEFF Research Database (Denmark)

    Persson, Sara; Jensen, Trine Hammer; Blomstrom, Anne-Lie

    2015-01-01

    years or older were infected. Few macroscopic changes were found upon necropsy. However, the relative weight of the spleen was sexually dimorphic and was found to be slightly, but significantly (rho = 0.006), heavier in AMDV infected male mink than uninfected. No association between AMDV infection.......1% of the mink were positive for AMDV antibodies and 57.6% were positive for AMDV DNA. Twenty-two percent of the mink tested on both tests (n = 133) had dissimilar results. The risk of having AMDV antibodies or being positive for AMDV DNA clearly increased with age and the majority of the mink that were two...

  15. Associations between biosecurity and outbreaks of canine distemper on Danish mink farms in 2012-2013

    DEFF Research Database (Denmark)

    Gregers-Jensen, Louise; Agger, Jens Frederik; Hammer, Anne Sofie Vedsted

    2015-01-01

    During 8 months from July 2012 to February 2013, a major outbreak of canine distemper involving 64 mink farms occurred on the Danish peninsula of Jutland. The canine distemper outbreak was associated with exposure of farmed mink to infected wild carnivores and could represent a deficit...... distemper from July 2012 to February 2013. The control group included 54 farms without an outbreak of canine distemper in 2012 or 2013, selected as the closest geographical neighbour to a case farm. The results showed that significantly more control than case farms had vaccinated their mink against canine...... distemper virus. Mortality was only assessed on the case farms, and there was a non-significantly lower mortality on vaccinated farms than on the non-vaccinated farms. Furthermore, the proportion of farms with observations of wild red foxes (Vulpes vulpes) inside the farm enclosures were larger for case...

  16. Tonawanda Creek, Genesee County, New York, Regional Flood Control. Final Environmental Impact Statement.

    Science.gov (United States)

    1981-11-01

    Long-tail Weasel : Mustela frenata Least Weasel : Mustela rixosa Mink : Mustela vison Eastern Skunk : Mephitis mephitis Red Fox : Vulpes fulva Gray Fo...environments and listed: (a) thickening of gill lamellae, (b) excessive mucous secretion, (c) abrasion of branchial epithelium, and (d) respiratory distress...Ranges Uhich May Include the Batavia Reservoir Compound Site Opossum : Didelphis marsupialis Raccoon : Procyon lotor Short-tail Weasel : Mustela erminea

  17. Fatty acid alterations caused by PCBs (Aroclor 1242) and copper in adipose tissue around lymph nodes of mink

    International Nuclear Information System (INIS)

    Kaekelae, R.; Hyvaerinen, H.

    1999-01-01

    Fatty acid composition was determined in adipose tissue surrounding the mesenteric lymph nodes of mink (Mustela vison) exposed to polychlorinated biphenyls (PCBs: 1 mg Aroclor 1242 in food day -1 for 28 days) and/or copper (62 mg kg -1 food). These specific adipose tissues are known to have functional relationships with lymphocytes, and proliferation of cultured lymphocytes is influenced by the quality of fatty acids available in media. In six experimental groups the diet was based on freshwater fish, and in two groups it was based on marine fish. These basal diets differed in terms of fatty acid composition and content of fat-soluble vitamins A 1 and E. The fatty acid composition of membrane phospholipids (PL) responded to PCBs more than that of triacylglycerols (TG). The effects of copper were small. In female minks fed a diet of freshwater fish, the proportion of highly unsaturated fatty acids in PL decreased by 5 wt.% due to PCBs, and the acids seemed to be replaced by monounsaturated fatty acids (9 wt.% increase of total). This decrease of highly unsaturated fatty acids in PL was milder in minks on the marine fish diet rich in fat-soluble vitamins. In TG of minks on the marine diet, however, PCBs decreased the proportion of docosahexaenoic acid (22:6n-3). The possibility that these alterations in the fatty acid metabolism of adipose tissue supporting the lymph nodes affect immune function during PCB exposure should be studied further. Interestingly, the quality of the fish diet affected the magnitude of the alterations. The fatty acid responses may also differ between males and females. (Copyright (c) 1999 Elsevier Science B.V., Amsterdam. All rights reserved.)

  18. High Prevalence of Aleutian Mink Disease Virus in Free-ranging Mink on a Remote Danish Island

    DEFF Research Database (Denmark)

    Jensen, Trine Hammer; Christensen, Laurids Siig; Chriél, Mariann

    2012-01-01

    Aleutian mink disease virus (AMDV) causes severe disease in farmed mink (Neovison vison) worldwide. In Denmark, AMDV in farmed mink has been confined to the northern part of the mainland since 2002. From 1998 to 2009, samples from 396 free-ranging mink were collected from mainland Denmark......, and a low AMDV antibody prevalence (3% of 296) was found using countercurrent immune electrophoresis. However, on the island of Bornholm in the Baltic Sea, a high prevalence (45% of 142 mink) was detected in the free-ranging mink. Aleutian mink disease virus was detected by polymerase chain reaction in 32...... of 49 antibody-positive free-ranging mink on Bornholm, but not in mink collected from other parts of Denmark. Sequence analysis of 370 base pairs of the nonstructural gene of the AMDV of 17 samples revealed two clusters with closest similarity to Swedish AMDV strains....

  19. QTL-mapping in mink (Neovison vison) shows evidence for QTL for guard hair thickness, guard hair length and skin length

    DEFF Research Database (Denmark)

    Thirstrup, Janne Pia; Labouriau, Rodrigo; Guldbrandtsen, Bernt

    2011-01-01

    Fur quality in mink (Neovison vison) is a composite trait, consisting of e.g. guard hair length, guard hair thickness and density of wool. A genome wide QTL search was performed to detect QTL for fur quality traits in mink. Here we present the results of QTL analyses for guard hair length, guard...... hair thickness and density of wool. Data from an F2-cross was analysed across fourteen chromosomes using 100 microsatellites as markers with a spacing of approximately 20 cM. The two lines used for the F2-cross were Nordic wild mink and American short nap mink. In total 1,083 animals (21 wild type, 25...... short nap, 103 F1 and 934 F2) were marker typed and recorded for the three presented fur quality traits. For the QTL-analyses a regression analysis implemented in QTL Express software was used. Evidence was found for the existence of QTL for guard hair length, guard hair thickness and density of wool...

  20. Environmental Impact Study of the Northern Section of the Upper Mississippi River. Pool 3.

    Science.gov (United States)

    1973-11-01

    Mustela frenata) Least Weasel ( Mustela rixosa) Mink ( Mustela vison ) Otter (Lutra canadensis) Spotted Skunk (Spilogale interrupta) Striped Skunk...Raccoon (Procyon lotor) Short-tailed Weasel ( Mustela erminea) i . . . . . . . . . . . ... 79 Table 15. Mammals of Pool 3 (Continued). Long-tailed Weasel... respiratory surfaces of both the fish in a fishecy, and the larger invertebrate prey of the fish. In spite of thi s, it might. be ]MS: detriMnt al to both types

  1. Balancing of protein and lipid intake by a mammalian carnivore, the mink, Mustela vison

    DEFF Research Database (Denmark)

    Mayntz, David; Nielsen, Vivi Hunnicke; Sørensen, Allan

    2009-01-01

    Many herbivores and omnivores can balance their intake of macronutrients when faced with nutritionally variable environments. Carnivores, however, are widely believed to optimize their rates of prey capture and energy intake rather than balancing nutrients. We tested nutrient balancing in captive...... target) of the two macronutrients. When given only one food of fixed nutrient composition, mink balanced macronutrient intake relative to the intake target, without showing the excessive energy intake on diets with a low percentage of protein and energy deficit on diets with a high percentage of protein...... previously reported for herbivores and omnivores, including humans. This demonstration of nutrient balancing in a carnivorous mammal indicates that the capacity for nutrient balancing is a more general phenomenon across trophic levels than was hitherto believed to be the case...

  2. A first estimate of the amino acid requirement for milk production of the high-producing female mink (Mustela vison)

    DEFF Research Database (Denmark)

    Fink, R; Tauson, A-H; Chwalibog, André

    2006-01-01

    Thirty mink dams nursing litters of six kits were assigned to one of three dietary treatments [high protein (HP), medium protein (MP) and low protein (LP)], fed ad libitum for 4 week from parturition, to investigate the effects of protein supply on milk yield and milk composition in order to esti...

  3. Effects of Aroclor 1242 and different fish-based diets on vitamins A(retinol) and A2 (3,4-didehydroretinol), and their fatty acyl esters in mink plasma

    International Nuclear Information System (INIS)

    Kaekelae, Anne; Kaekelae, Reijo; Hyvaerinen Heikki; Nieminen, Petteri

    2003-01-01

    The effects of a 21-week exposure to Aroclor 1242 (1 mg per day in feed on plasma concentrations of vitamins A 1 (retinol) and A(3,4-didehydroretinol) and their principal fatty acyl esters (A 1 -16:0 A 2 -16:0 (palmitates), A 1 -18:1n-9; A 2 -18:1n-9 (oleates), and A 1 -18:0 A 2 -18:0 (stearates)) were studied in young female mink (Mustela vison) fed a diet based on freshwater smelt. These vitamin levels were also examined in mink fed diets containing Baltic herring or fatty marine fish. In the Aroclor-exposed smelt-fed mink, the plasma concentrations of A 1 and Aesters were significantly lower than the levels in controls fed the uncontaminated smelt diet. In addition, the A 2 esters reacted mor sensitively to the polychlorinated biphenyls than did A 1 esters. I contrast, in the plasma of the exposed mink the level of alcoholic A 1 was normal, and transport of thyroxine (T 4 ) and nonspecific lipoprotei transport of major lipids were not impaired. Despite the large dietary supply of vitamin A 2 and high levels of plasma A 2 esters, the mink fed freshwate smelt had only trace amounts of alcoholic vitamin A 2 in their plasma. The concentrations of A 1 and A 2 esters in the plasma of all the mink studied correlated with the hepatic total concentrations of the vitamins. Thus, in carnivores that have nonspecific lipoprotein transport of vitamin A esters determination of plasma levels of the esterified vitamins may be a useful nondestructive way to estimate stores of the vitamin A analogs in the body and to assess the organochlorine-induced decrease in the vitamin stores

  4. WelFur - mink: development of on-farm welfare assessment protocols for mink

    DEFF Research Database (Denmark)

    Møller, Steen Henrik; Hansen, Steffen W; Rousing, Tine

    2012-01-01

    European Fur Breeder's Association initiated the "WelFur" project in 2009 in order to develop a welfare assessment protocol for mink and fox farms after the Welfare Quality® standards. The assessment is based on four welfare principles (Good feeding, good housing, good health and appropriate beha...... mink production seasons: Winter, spring, and autumn, in order to cover the life cycle of mink and proved feasible for a one-day visit.......European Fur Breeder's Association initiated the "WelFur" project in 2009 in order to develop a welfare assessment protocol for mink and fox farms after the Welfare Quality® standards. The assessment is based on four welfare principles (Good feeding, good housing, good health and appropriate...

  5. The status of the American mink (Neovison vison) in the Netherlands

    NARCIS (Netherlands)

    Dekker, J.J.A.; Hofmeester, T.R.

    2014-01-01

    The American mink (Neovison vison) is a north American mustelid that has been farmed for its fur in Europe since the 1920s. It has been feral in the Netherlands since 1958. This paper discusses its distribution, diet, the indications for reproduction, and whether feral animals are born in the wild

  6. Mink's adaptation to group housing in practice

    DEFF Research Database (Denmark)

    Hansen, Steffen W; Møller, Steen Henrik

    2012-01-01

    In this project, we test the hypothesis whether mink populations with a higher social tolerance have been developed in practice and thus are better adapted to housing in stacked cages. The hypothesis has been tested by comparing the level of bite damages and bit marks in mink kept in pairs...... and in mink kept in social groups. In total, the project included 1,191 brown mink housed on four farms in up to five different types of social groups. The result showed that mink in groups had significantly more bite marks than mink kept in pairs, and that mink with bite wounds had significantly more bite...... marks than mink that did not haev bite wounds. Thus, based upon bite mark results, we cannot confirm the hypothesis that mink populations adapted to group housing have been developed. The number of mink that had died or had been removede during the test period, or that had bite wounds at the time...

  7. The vomeronasal system of the mink, Mustela vison. I. The vomeronasal organ.

    Science.gov (United States)

    Salazar, I; Cifuentes, J M; Quinteiro, P S; Caballero, G

    1994-01-01

    The vomeronasal organ (VNO) of the mink is restricted to the area of the Fissura palatina and thus always topographically related to the Ductus incisivus (DI). The VNO and DI have also a functional relation because the vomeronasal duct ends in the incisive duct. On the other hand, as the DI has its mouth in the Papilla incisiva there is a communication between the VNO and the oral cavity. The vomeronasal cartilage, approximately in 1/6 of its length, wraps completely the parenchyma of the VNO whose main structure is the vomeronasal duct, with two different epithelia: sensory receptor in the medial wall, and respiratory in the lateral one. Nevertheless the variations of epithelia belong to the segment of the duct because in its rostral and caudal parts the medial and lateral epithelia are very similar. Branches and tubular PAS positive glands stand out in three points: superior, inferior and medial areas of the vomeronasal duct in which they end. An important number of vessels, mainly veins of different diameter, are located around the duct, while the nervous fibers are close to the medial wall of the duct and very easy to identify in the caudal third of the organ. Two immunohistochemical techniques were used to identify the nerve fibers and the receptor cells.

  8. Mink kan også have influenza

    DEFF Research Database (Denmark)

    Hjulsager, Charlotte Kristiane; Krog, Jesper Schak; Larsen, Gitte

    2017-01-01

    , hvis der opstår mistanke om influenza ved obduktionen, eller hvis der er alvorlige langvarige udbrud. For at kunne iværksætte foranstaltninger, der begrænser forekomsten af influenza hos mink, er det nødvendigt at kende udbredelsen af influenzavirus blandt farmede mink i Danmark. Formålet med denne...... minkobduktionskursus, samt vilde mink. Der blev påvist influenza A virus i mink fra otte farme. Genetiske analyser indikerede, at disse virus stammede fra både danske svin og mennesker. For at forebygge udbrud af influenza i farmede mink anbefales det, at undgå kontakt mellem mink og influenzasyge personer, samt sikre...

  9. Effect of Helicobacter mustelae infection on ferret gastric epithelial cell proliferation.

    Science.gov (United States)

    Yu, J; Russell, R M; Salomon, R N; Murphy, J C; Palley, L S; Fox, J G

    1995-08-01

    The effect of Helicobacter mustelae infection on gastric epithelial proliferation was studied in ferrets colonized with H.mustelae and specific pathogen-free (SPF) ferrets not infected with H.mustelae. Thirteen H. mustelae-infected ferrets between the ages of 13 and 32 months and 16 SPF ferrets between 6 and 18 months were analyzed. Bacterial cultures, urease tests and Warthin-Starry stains were used to identify H.mustelae. Tissues obtained from the antrum and the body regions of the stomach were assayed by proliferating cell nuclear antigen (PCNA) immunohistochemistry and measured using a computerized color image analysis system. PCNA-expressing gastric epithelia in the antrum and the body regions were significantly increased in the H.mustelae-infected ferrets versus the SPF ferrets (P < 0.001). PCNA positivity in the antrum regions of both the H.mustelae-infected ferrets and SPF ferrets was significantly higher than that of the body regions (P < 0.001). Comparison of the histopathology of infected ferrets indicated that PCNA positivity correlated with the histological severity of gastritis. This study suggests that cell proliferation in ferret gastric mucosa increases with H.mustelae infection and provides evidence that PCNA is a useful biomarker for studying the changes in cell kinetics in the ferret stomach. The data also further support the use of the H.mustelae-infected ferret as an animal model for studying the pathogenesis of Helicobacter pylori-induced gastric diseases of humans.

  10. Reproductive parameters of critically endangered European mink (Mustela lutreola) in captivity.

    Science.gov (United States)

    Kiik, Kairi; Maran, Tiit; Nemvalts, Kristel; Sandre, Siiri-Lii; Tammaru, Toomas

    2017-06-01

    Founding captive populations is often the last chance for saving endangered species from extinction. Ensuring successful reproduction is typically most critical for the maintenance of captive populations, with purposeful selection of individuals for breeding being one of the crucial aspects. Comparable cross-species data on the determinants of reproduction success are most useful for solving problems in captive species programs. In the present study, we provide an overview of a 20-year captive breeding program of the critically endangered European mink. The mating season starts in March, reaching its peak in the middle of April. The average gestation length was 43.8days (mode 43), the mean litter size being 4.4 (mode 4). Litter size and cub survival were negatively correlated with maternal age but this effect was entirely due to the lower performance of the females over 4 years of age. Female body weight also showed a positive correlation with litter size, with the weight itself having increased by 10% during the 20- year period. We did not find any signs of a cost of reproduction: the number of litters the female had delivered earlier in her life did not have an effect on her litter size in the focal year. Beyond the effect of age and size, individual females did not differ in litter sizes. Consistently, we found the heritability of litter size to be low. We conclude that, when selecting females for breeding, there is little need to consider aspects other than genetic relatedness crucial for avoiding progressive inbreeding. Copyright © 2017 Elsevier B.V. All rights reserved.

  11. Predation of the alien American mink Mustela vison on native crayfish populations in middle-sized streams in central and western Bohemia

    Czech Academy of Sciences Publication Activity Database

    Fischer, D.; Pavluvčík, P.; Sedláček, František; Šálek, Martin

    2009-01-01

    Roč. 58, č. 1 (2009), s. 45-56 ISSN 0139-7893 Institutional research plan: CEZ:AV0Z60870520 Keywords : mink diet * stone crayfish * prey selectivity * density-dependent predation * Czech Republic Subject RIV: EG - Zoology Impact factor: 0.357, year: 2009

  12. Changes in digestive enzyme activity, intestine morphology, mucin characteristics and tocopherol status in mink kits (Mustela neovision) during the weaning period

    DEFF Research Database (Denmark)

    Hedemann, Mette Skou; Clausen, T.N.; Jensen, Søren Krogh

    2011-01-01

    Weaning of livestock mammals is often associated with digestive problems related to profound changes in the physiology of the gastrointestinal tract. This study was undertaken to study the developmental changes in the gastrointestinal tract of mink kits during the period of 34 to 59 days of age. ...

  13. Two parvoviruses that cause different diseases in mink have different transcription patterns: Transcription analysis of mink enteritis virus and Aleutian mink disease parvovirus the same cell line

    DEFF Research Database (Denmark)

    Storgaard, T.; Oleksiewicz, M.; Bloom, M.E.

    1997-01-01

    The two parvoviruses of mink cause very different diseases, Mink enteritis virus (MEV) is associated with rapid, high-level viral replication and acute disease, In contrast, infection with Aleutian mink disease parvovirus (ADV) is associated with persistent, low-level viral replication and chronic...

  14. Growh performance, nitrogen balance and urinary purine derivatives in growing-furring mink (Mustela vison) fed bacterial protein produced from natural gas

    DEFF Research Database (Denmark)

    Ahlstrøm, Ø.; Tauson, Anne-Helene; Hellwing, Anne Louise Frydendahl

    2006-01-01

    A bacterial protein meal (BPM), containing 70% crude protein and produced on natural gas, was evaluated versus fish meal as protein source for mink in the growing-furring period (June 29-November 26). BPM, rich in nucleic acids, accounted for 0 (control), 20 and 40% of dietary crude protein...

  15. Man's underground best friend: domestic ferrets, unlike the wild forms, show evidence of dog-like social-cognitive skills.

    Science.gov (United States)

    Hernádi, Anna; Kis, Anna; Turcsán, Borbála; Topál, József

    2012-01-01

    Recent research has shown that dogs' possess surprisingly sophisticated human-like social communication skills compared to wolves or chimpanzees. The effects of domestication on the emergence of socio-cognitive skills, however, are still highly debated. One way to investigate this is to compare socialized individuals from closely related domestic and wild species. In the present study we tested domestic ferrets (Mustela furo) and compared their performance to a group of wild Mustela hybrids and to domestic dogs (Canis familiaris). We found that, in contrast to wild Mustela hybrids, both domestic ferrets and dogs tolerated eye-contact for a longer time when facing their owners versus the experimenter and they showed a preference in a two-way choice task towards their owners. Furthermore, domestic ferrets, unlike the wild hybrids, were able to follow human directional gestures (sustained touching; momentary pointing) and could reach the success rate of dogs. Our study provides the first evidence that domestic ferrets, in a certain sense, are more dog-like than their wild counterparts. These findings support the hypothesis that domestic species may share basic socio-cognitive skills that enable them to engage in effectively orchestrated social interactions with humans.

  16. Man's underground best friend: domestic ferrets, unlike the wild forms, show evidence of dog-like social-cognitive skills.

    Directory of Open Access Journals (Sweden)

    Anna Hernádi

    Full Text Available Recent research has shown that dogs' possess surprisingly sophisticated human-like social communication skills compared to wolves or chimpanzees. The effects of domestication on the emergence of socio-cognitive skills, however, are still highly debated. One way to investigate this is to compare socialized individuals from closely related domestic and wild species. In the present study we tested domestic ferrets (Mustela furo and compared their performance to a group of wild Mustela hybrids and to domestic dogs (Canis familiaris. We found that, in contrast to wild Mustela hybrids, both domestic ferrets and dogs tolerated eye-contact for a longer time when facing their owners versus the experimenter and they showed a preference in a two-way choice task towards their owners. Furthermore, domestic ferrets, unlike the wild hybrids, were able to follow human directional gestures (sustained touching; momentary pointing and could reach the success rate of dogs. Our study provides the first evidence that domestic ferrets, in a certain sense, are more dog-like than their wild counterparts. These findings support the hypothesis that domestic species may share basic socio-cognitive skills that enable them to engage in effectively orchestrated social interactions with humans.

  17. 9 CFR 113.302 - Distemper Vaccine-Mink.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Distemper Vaccine-Mink. 113.302... Virus Vaccines § 113.302 Distemper Vaccine—Mink. Distemper Vaccine—Mink shall be prepared from virus... follows: (1) To detect virulent canine distemper virus, each of two distemper susceptible mink or ferrets...

  18. Environmental Impact Statement. Disposal and Reuse of Wurtsmith Air Force Base, Michigan

    Science.gov (United States)

    1993-09-01

    Mephitis mephitis Meadow vole Microtus pennsy/vanicus House mouse Mus musculus Long-tailed weasel Mustela frenata Mink Mustela vison Little brown bat...fuels, such as coal and oil, are burned. SO2 is the main pollutant involved in the formation of acid rain. SO also can irritate the upper respiratory

  19. Effect of dietary protein levels on growth performance, mortality rate and clinical blood parameters in mink (Mustela vison)

    DEFF Research Database (Denmark)

    Damgaard, B.M.; Clausen, T.N.; Dietz, Hans Henrik

    1998-01-01

    Effects of dietary protein levels ranging from 35% to 15% of metabolizable energy (ME) and dietary fat levels ranging in a reciprocal fashion from 47% to 67% of ME, and a constant dietary carbohydrate level of 18% of ME were investigated in male mink kits in the growing-furring period. Growth...... performance, mortality rate, hepatic fatty infiltration, weights of body and liver, relative weight of liver, haematocrit values, plasma activities of alanine-aminotransferase (ALAT), aspartate-aminotransferase (ASAT) and creatine-kinase (CK), and plasma concentrations of chemical parameters were studied...

  20. Environmental Impact Analysis Process. Draft Environmental Impact Statement Proposed Central Radar System Over-the-Horizon Backscatter Radar Program

    Science.gov (United States)

    1986-08-01

    Other wetland-dependent mammals include beaver (Castor canadenis) and mink ( Mustela vison ). The eastern cottontail and white-tailed jack rabbit are...least weasel ( Mustela nivalis), and the long-tailed weasel ( Mustela frenata). Several mammals are valuable furbearers. According to the NDGFD, the species...human skull. One study has reported small changes in brain tissue respiratory chain function at a power density of 5 mW/cm 2 . It is unlikely that such

  1. Environmental Impact Analysis Process. Draft Environmental Impact Statement Proposed Alaskan Radar System Over-the-Horizon Backscatter Radar Program

    Science.gov (United States)

    1986-08-01

    Mustela vison wolverine Gulo Kulo river otter Lutra canadensis lynx Lynx canadensis moose Alces alces caribou Rangifer tarandus Dall’s sheep Ovic dalli...tissue respiratory chain function at a power density of 5 mW/cm2 . It is unlikely that such effects would be detectable at the power densities at ground...Vulves vulpes black bear Ursus americanus grizzly bear Ursus arctos marten Martes americana ermine Mustela erminea least weasel Mustela nivalis mink

  2. Molecular characterization of H9N2 influenza virus isolated from mink and its pathogenesis in mink.

    Science.gov (United States)

    Peng, Li; Chen, Chen; Kai-yi, Han; Feng-xia, Zhang; Yan-li, Zhu; Zong-shuai, Ling; Xing-xiao, Zhang; Shi-jin, Jiang; Zhi-jing, Xie

    2015-03-23

    In mid-August 2013, two H9N2 influenza viruses, named A/mink/Shandong/F6/2013 (Mk/SD/F6/13) and A/mink/Shandong/F10/2013 (Mk/SD/F10/13), were isolated from lung samples of 2 of 45 farmed mink exhibiting respiratory signs in mideastern Shandong province, China. The seroprevalence of antibodies to H9N2 in mink was 20% (53/265). Based on sequence analysis, the eight nucleotide sequences showed 99.7-100% identity between Mk/SD/F6/13 and Mk/SD/F10/13. The HA, NP and NS genes of Mk/SD/F6/13 and Mk/SD/F10/13 were close to A/chicken/Zhejiang/329/2011 (H9N2), the NA and PB1 genes to A/duck/Hunan/S4111/2011 (H9N2), the PA and M genes to A/chicken/Shanghai/C1/2012 (H9N2). However, the PB2 genes had a close relationship with A/Turkey/California/189/66 (H9N2). Based on Sialic acid (SA) receptor detection, a range tissues of the mink demonstrated staining for MAA and/or SNA, and mink could serve as an intermediate host for influenza viruses with pandemic potential for the other animals. Experimental infection of mink demonstrated that mink could be infected by H9N2 influenza viruses and presented mild clinical signs, virus shedding and seroconversion, but no animals died of the disease. It implied that mammalian host-adapted avian H9N2 strains infected mink. Copyright © 2015 Elsevier B.V. All rights reserved.

  3. Acoustic Ecology of Minke Whales

    National Research Council Canada - National Science Library

    Costa, Daniel

    1998-01-01

    Calls of minke whales, Balaenoptera acutorostrata, off the coast of NE Australia in July of 1997 were recorded utilizing a newly described population, where minke whales maintain long contacts with vessels...

  4. Måling af stress hos mink

    DEFF Research Database (Denmark)

    Malmkvist, Jens

    2010-01-01

    Sammendrag Stress indgår som et element ved vurdering af dyrevelfærd. Endvidere kan stress mål anvendes i undersøgelser, hvor forskellige typer af avl, burmiljø og pasning af mink sammenlignes. Men hvad er stress egentlig og hvordan måles det? Er unormal adfærd koblet til stress hos mink? På...... temadagen præsenteres nye resultater og metoder til at vurdere stress hos mink. I en metode måles koncentrationen af nedbrydningsprodukter af hormonet cortisol i gødning fra mink. Der er en række fordele ved denne metode, frem for blodprøvetagning. Forsøg viste, at hovedparten af cortisol udskilles i fæces...... (83 %) frem for i urin (17 %), samt at omsætningen af radioaktivt mærket cortisol ikke er forskellig mellem mink med hhv. lav og høj forekomst af stereotypi. Denne viden kan bruges til bedre at forstå koblingen f.eks. unormal adfærd og stress hos mink....

  5. Late development of homoeothermy in mink (Mustela vison) kits - a strategy for maximum survival rate

    DEFF Research Database (Denmark)

    Tauson, A-H; Chwalibog, André; Tygesen, M P

    2006-01-01

    and after the experiments and evaporative water losses (EWL) were calculated. When exposed to L temperature, single kits responded with a very low HE until 29 days of age, and groups of kits until 14 days of age. It was not until they reached an age of approximately 6 weeks that single kits showed a clear...... thermoregulatory response to the L temperature by increased HE, whereas groups of kits showed increased HE from 29th day onwards. When kept at H temperature, HE was low initially, but all kits showed elevated HE at 8 days of age, and the metabolic rate was similar for single kits and kits huddling in groups....... Evaporative water losses was higher among single than among groups of kits and slightly lower but more variable for animals at L than at H temperature. It was concluded that mink kits develop functional homoeothermy at an age of close to 6 weeks and that the failure of very young kits to thermoregulate...

  6. Genomics using the Assembly of the Mink Genome

    DEFF Research Database (Denmark)

    Guldbrandtsen, Bernt; Cai, Zexi; Sahana, Goutam

    2018-01-01

    The American Mink’s (Neovison vison) genome has recently been sequenced. This opens numerous avenues of research both for studying the basic genetics and physiology of the mink as well as genetic improvement in mink. Using genotyping-by-sequencing (GBS) generated marker data for 2,352 Danish farm...... mink runs of homozygosity (ROH) were detect in mink genomes. Detectable ROH made up on average 1.7% of the genome indicating the presence of at most a moderate level of genomic inbreeding. The fraction of genome regions found in ROH varied. Ten percent of the included regions were never found in ROH....... The ability to detect ROH in the mink genome also demonstrates the general reliability of the new mink genome assembly. Keywords: american mink, run of homozygosity, genome, selection, genomic inbreeding...

  7. Postvaccination wounds associated predominantly with Arcanobacterium phocae in mink (Neovison vison) at three mink farms.

    Science.gov (United States)

    Molenaar, Robert J; Buter, Rianne; Sroka, Agnieszka

    2017-04-01

    The emerging skin disease fur animal epidemic necrotic pyoderma (FENP) has been attributed to infection with Arcanobacterium phocae (ABP). The exact pathogenesis and risk factors of FENP have yet to be elucidated. Three mink from each of three different mink farms (A-C) with postvaccination skin wounds at the vaccination site and six mink from an unaffected mink farm (D) that had used the same vaccine batch and vaccination site (hind leg). All mink from farms A-C had severe necrotizing to necropurulent dermatitis where they were vaccinated intramuscularly in the hind leg. ABP was the sole bacterium cultured from six of nine wounds. Using 16S-23S rDNA intergenic spacer region and BOX-PCR, the ABP isolates from these wounds were indistinguishable from isolates originating from several cases of FENP. This is the first report of FENP-like lesions at the site of vaccination, in the days following the procedure, associated with ABP. At farms with FENP vaccination, procedures should be considered carefully. © 2016 ESVD and ACVD.

  8. Associations between biosecurity and outbreaks of canine distemper on Danish mink farms in 2012-2013.

    Science.gov (United States)

    Gregers-Jensen, Louise; Agger, Jens Frederik; Hammer, Anne Sofie Vedsted; Andresen, Lars; Chrièl, Mariann; Hagberg, Emma; Jensen, Mette Kragh; Hansen, Mette Sif; Hjulsager, Charlotte Kristiane; Struve, Tina

    2015-09-30

    During 8 months from July 2012 to February 2013, a major outbreak of canine distemper involving 64 mink farms occurred on the Danish peninsula of Jutland. The canine distemper outbreak was associated with exposure of farmed mink to infected wild carnivores and could represent a deficit in biosecurity on the mink farms. The aim of this study was to investigate the extent and association of specific biosecurity measures with the outbreak. The study was carried out in an epidemiological case-control design. The case group consisted of the 61 farms, which had a confirmed outbreak of canine distemper from July 2012 to February 2013. The control group included 54 farms without an outbreak of canine distemper in 2012 or 2013, selected as the closest geographical neighbour to a case farm. The results showed that significantly more control than case farms had vaccinated their mink against canine distemper virus. Mortality was only assessed on the case farms, and there was a non-significantly lower mortality on vaccinated farms than on the non-vaccinated farms. Furthermore, the proportion of farms with observations of wild red foxes (Vulpes vulpes) inside the farm enclosures were larger for case farms, indicating that the control farms had a better biosecurity or were not equally exposed to canine distemper virus. Generally, all farms had very few specific precautions at the gate entrance in respect to human visitors as well as animals. The use of biosecurity measures was very variable in both case and control farms. Not using plastic boot covers, presence of dogs and cats, presence of demarcated area for changing clothes when entering and leaving the farm area and presence of hand washing facilities significantly lowered the odds of the farm having a canine distemper virus outbreak. The results of the study indicate that consistent use of correct vaccination strategies, implementation of biosecurity measures and limiting human and animal access to the mink farm can be

  9. Arcanobacterium phocae infection in mink (Neovison vison), seals (Phoca vitulina, Halichoerus grypus) and otters (Lutra lutra)

    DEFF Research Database (Denmark)

    Nonnemann, Bettina; Chriél, Mariann; Larsen, Gitte

    2017-01-01

    pathological conditions, and from wild seals and otters. In 2015, A. phocae was isolated for the first time in Denmark from outbreaks of dermatitis in mink farms. The outbreaks affected at least 12 farms. Originating from these 12 farms, 23 animals cultured positive for A. phocae. The main clinical findings......Infectious skin disorders are not uncommon in mink. Such disorders are important as they have a negative impact on animal health and welfare as well as on the quality and value of the fur. This study presents the isolation of Arcanobacterium phocae from mink with severe skin lesions and other...... were necrotizing pododermatitis or dermatitis located to other body sites, such as the lumbar and cervical regions. A. phocae could be isolated from skin lesions and in nine animals also from liver, spleen and lung, indicating a systemic spread. The bacterium was also, for the first time in Denmark...

  10. State of Washington, Aquatic Plant Management Program: Design Memorandum.

    Science.gov (United States)

    1979-10-01

    Ondatra sibethicus Mink Mustela vison Striped skunk Mephitis mephitis Raccoon Procyon lotor Marten Martes americana River otter Lutra canadensis Long...tailed weasel Mustela frenata Badger Taxidea taxus Little brown Mrotis yotis lucifugus California myotis _yotis californicus Yuma nyotis Myotis...occupational, and medical histories, paying par- ticular attention to respiratory , gastrointestinal, dermatological, and neurological systems. " Physical

  11. Comparative genomics and proteomics of Helicobacter mustelae, an ulcerogenic and carcinogenic gastric pathogen

    LENUS (Irish Health Repository)

    O'Toole, Paul W

    2010-03-10

    Abstract Background Helicobacter mustelae causes gastritis, ulcers and gastric cancer in ferrets and other mustelids. H. mustelae remains the only helicobacter other than H. pylori that causes gastric ulceration and cancer in its natural host. To improve understanding of H. mustelae pathogenesis, and the ulcerogenic and carcinogenic potential of helicobacters in general, we sequenced the H. mustelae genome, and identified 425 expressed proteins in the envelope and cytosolic proteome. Results The H. mustelae genome lacks orthologs of major H. pylori virulence factors including CagA, VacA, BabA, SabA and OipA. However, it encodes ten autotransporter surface proteins, seven of which were detected in the expressed proteome, and which, except for the Hsr protein, are of unknown function. There are 26 putative outer membrane proteins in H. mustelae, some of which are most similar to the Hof proteins of H. pylori. Although homologs of putative virulence determinants of H. pylori (NapA, plasminogen adhesin, collagenase) and Campylobacter jejuni (CiaB, Peb4a) are present in the H. mustelae genome, it also includes a distinct complement of virulence-related genes including a haemagglutinin\\/haemolysin protein, and a glycosyl transferase for producing blood group A\\/B on its lipopolysaccharide. The most highly expressed 264 proteins in the cytosolic proteome included many corresponding proteins from H. pylori, but the rank profile in H. mustelae was distinctive. Of 27 genes shown to be essential for H. pylori colonization of the gerbil, all but three had orthologs in H. mustelae, identifying a shared set of core proteins for gastric persistence. Conclusions The determination of the genome sequence and expressed proteome of the ulcerogenic species H mustelae provides a comparative model for H. pylori to investigate bacterial gastric carcinogenesis in mammals, and to suggest ways whereby cag minus H. pylori strains might cause ulceration and cancer. The genome sequence was

  12. Analysis of experimental mink enteritis virus infection in mink: in situ hybridization, serology, and histopathology

    DEFF Research Database (Denmark)

    Uttenthal, Åse; Larsen, S; Lund, E

    1990-01-01

    Strand-specific hybridization probes were used in in situ hybridization studies to localize cells containing mink enteritis virus (MEV) virion DNA or MEV replicative-form DNA and mRNA. Following the experimental MEV infection of 3-month-old unvaccinated mink, a significant increase in serum antib...

  13. Radiation pasteurization of mink feed

    International Nuclear Information System (INIS)

    Passey, C.A.; Roy, D.; Savoie, L.; Wilson, J.

    1990-01-01

    No significant differences were observed in the net birth rate of mink kits/female between the 7 breeding groups. However, there was reduced incidence (P=0.05) of kit deaths among the females receiving irradiated feed, and larger kit size (P<0.0001) at birth particularly for the litter size of 5-8 kits. The second generation minks born to parents receiving feed irradiated to a planned dose of 1 kGy weighed on average about 2.5% more, and their fur was on average about 1±0.26 cm longer (12% more males making the top length grade). Moreover, there was no effect of irradiated feed on fur quality. Irradiation of mink feed with subsequent frozen storage of the meat component improved the microbiological quality by decreasing the incidence of Pseudomonas sp. and Salmonella sp. Radiation pasteurization of mink feed (frozen meat to 1 kGy, and dry feed to 2 kGy or more) should therefore help improve feed utilization, keep animals healthier, and reproducing better without affecting fur quality. (author)

  14. U. S. Navy Deepening of Pinole Shoal and Mare Island Strait Regulatory Permit Application by the Commander, Mare Island Shipyard, Solano County, California.

    Science.gov (United States)

    1981-07-01

    Mustela vison Mink Mustela . frenata Long-Tailed WJeasel M e hi is mpii*Striped Skunk Order Artiodactyla Odocoileus_ hemqionus Mule Deer Birds Order...intermitted nature of operation. 10 c. Fish - temporary adverse impact on respiratory structures (i.e. inhibition of respiratory exchange through... respiratory structures (i.e. inhibition of respiratory exchange through clogging of gills and the abrasive action on gill filaments) and feeding processes

  15. Lead poisoning in mink

    Energy Technology Data Exchange (ETDEWEB)

    Purdy, J G

    1962-03-01

    This paper describes a case of lead poisoning in minks. The mink were housed in pens which had been painted with a bridge paint containing lead. They had chewed on the pen and ingested the paint. The animals that did not die were moved to new pens, and vitamin D and calcium gluconate were added to their diets. In three days, a marked improvement was seen in the food and water consumption, and convolutions became less frequent.

  16. The draft genome sequence of the American mink (Neovison vison) opens new opportunities of genomic research in mink

    DEFF Research Database (Denmark)

    Cai, Zexi; Panitz, Frank; Petersen, Bent

    2016-01-01

    The American mink (Neovison vison) is a semiaquatic mustelid native to North America. It is an important animal for the fur industry. Although many efforts have been made to locate genes influencing fur quality and color, the lack of a reference genome impedes the search. American mink has...... of Carnivora. Here we present the draft genome sequence of American mink. In our study, a male inbred pearl mink was sequenced by Illumina paired-end and mate pair sequencing. The reads were assembled, which lead to 22,419 scaffolds with an N50 (shortest sequence length at 50% of the genome) of 646,304 bp...

  17. Stress influencing production and welfare in farmed mink

    DEFF Research Database (Denmark)

    Malmkvist, Jens

    2012-01-01

    Results linking stress, production and welfare in farmed mink, with special focus on the hypothalamic-pituitary-adrenal (HPA)-axis, are presented. Interpretation of stress responses are discussed, combining context, neuroendocrine and behavioural indicators. Results from the development and valid......Results linking stress, production and welfare in farmed mink, with special focus on the hypothalamic-pituitary-adrenal (HPA)-axis, are presented. Interpretation of stress responses are discussed, combining context, neuroendocrine and behavioural indicators. Results from the development...... animals and whether fur-chewing/stereotypic behaviour is associated with hippocampal neurogenesis in mink. Perspectives and suggested future research aiming to improve production and welfare in farmed mink are given....

  18. 9 CFR 113.204 - Mink Enteritis Vaccine, Killed Virus.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Mink Enteritis Vaccine, Killed Virus..., DEPARTMENT OF AGRICULTURE VIRUSES, SERUMS, TOXINS, AND ANALOGOUS PRODUCTS; ORGANISMS AND VECTORS STANDARD REQUIREMENTS Killed Virus Vaccines § 113.204 Mink Enteritis Vaccine, Killed Virus. Mink Enteritis Vaccine...

  19. Observaciones sobre el plan de vacunación contra el distemper en visones (Mustela vison en un criadero de Argentina Evaluation of the immune response after vaccination against distemper at a mink (Mustela vison farm in Argentina

    Directory of Open Access Journals (Sweden)

    A. M. Jar

    2010-09-01

    Full Text Available El virus del distemper produce una enfermedad en los visones que cursa con síntomas respiratorios, gastrointestinales, neurológicos y cutáneos y presenta alta morbilidad y mortalidad, principalmente en los cachorros. La enfermedad se controla mediante la aplicación de vacunas destinadas a esta especie. El objetivo del trabajo fue determinar el título de anticuerpos neutralizantes contra el virus del distemper en visones vacunados y no vacunados de un criadero de Argentina. Se analizó la cinética de anticuerpos obtenidos posvacunación en 27 animales adultos y de anticuerpos calostrales en 10 cachorros. Todos los visones adultos vacunados mostraron títulos protectores hasta por lo menos 3 meses después de la vacunación. El 37,5% redujo significativamente sus niveles de anticuerpos a los 12 meses de la vacunación. En los cachorros, sólo un 20% mostró niveles protectores de anticuerpos calostrales a las 7 semanas de edad, que se negativizaron hacia las 11 semanas. La vacunación a las 13 semanas de edad produjo títulos protectores en todos estos animales. Estos resultados muestran que la vacunación induce respuesta humoral satisfactoria en el medio estudiado e indican la conveniencia de revacunar a las madres anualmente antes del servicio. Se discute el plan de vacunación en cachorros.Distemper virus causes a disease affecting minks with respiratory, gastrointestinal, neurological and skin symptoms and showing high morbidity and mortality, mainly among puppies. It is controlled through immunization, using vaccines that are supplied for mink use. The aim of this work was to determine the seroneutralization titer against the distemper virus at a mink farm in Argentina. The antibody kinetics obtained after vaccination in 27 adult animals, as well as the duration of colostrum-transferred antibodies in 10 puppies were determined. All vaccinated adult minks showed protective titers up to at least 3 months after vaccination, and 37

  20. Progression of experimental chronic Aleutian mink disease virus infection

    DEFF Research Database (Denmark)

    Jensen, Trine Hammer; Chriél, Mariann; Hansen, Mette Sif

    2016-01-01

    Aleutian mink disease virus (AMDV) is found world-wide and has a major impact on mink health and welfare by decreasing reproduction and fur quality. In the majority of mink, the infection is subclinical and the diagnosis must be confirmed by serology or polymerase chain reaction (PCR). Increased ...

  1. Chromosomal and regional localization of the loci for IGKC, IGGC, ALDB, HOXB, GPT, and PRNP in the American mink (Mustela vison): comparisons with human and mouse

    DEFF Research Database (Denmark)

    Khlebodarova, TM; Malchenko, Sergey; Matveeva, NM

    1995-01-01

    Chromosomal localization of the genes for gamma- and kappa-immunoglobulins (IGGC and IGKC, respectively), aldolase B (ALDB), prion protein (PRNP), homeo box B (HOXB), and glutamate pyruvate transaminase (GPT) were determined with the use of mink-rodent hybrid cells. Analysis of segregation...

  2. WelFur-mink: on-farm welfare assessment of mink (Neovision vision) - effect of sample size on animal based measures

    DEFF Research Database (Denmark)

    Rousing, Tine; Møller, Steen Henrik; Hansen, Steffen W

    2012-01-01

    European Fur Breeder's Association initiated the "WelFur project" in 2009 which is aiming at developing an applicable on farm welfare assessment protocol for mink based on the Welfare Quality® principles. Such a welfare assessment system should possess the following qualities: It should be "high...... measures such as "stereotypy", "tremperament" and "body condition". These measures were in each fo 9 Danish mink farms on approximately 250 adult mink on each farm collected during the nursing season 2011 by a total of 8 observers working in pairs. Descriptive result showed a large between herd variations...

  3. Dietary Lipids, Cell Adhesion and Breast Cancer Metastasis

    Science.gov (United States)

    2003-10-01

    Mustela vison ): a light and electron microscopic study. APMIS 106. 785-799.caspase activity and apoptosis in neuronal cells (Francois et suy PI...N.Y. Acad. Sci. 919, 97-105. tic lesions in the mink ( Mustela vison ): A light and electron microscopic Krause, T., and Turner, G. A. (1999). Are...lipid composition is the most critical factor determining HUVEC with a plasmid regulated by a strong respiratory the efficiency of liposome-mediated

  4. Radiation pasteurization of mink feed; Effect of irradiated feed on reproductive performance, growth and fur quality of mink

    Energy Technology Data Exchange (ETDEWEB)

    Passey, C A; Roy, D; Savoie, L [St. Hyacinthe Food Research Centre, Agriculture (Canada); Malo, R [AgriZo Vet Consultants, St. Hyacinthe, QA (Canada); Wilson, J [J.J. Wilson et Fils, Inc., St. Hyacinthe, QA (Canada)

    1990-01-01

    No significant differences were observed in the net birth rate of mink kits/female between the 7 breeding groups. However, there was reduced incidence (P=0.05) of kit deaths among the females receiving irradiated feed, and larger kit size (P<0.0001) at birth particularly for the litter size of 5-8 kits. The second generation minks born to parents receiving feed irradiated to a planned dose of 1 kGy weighed on average about 2.5% more, and their fur was on average about 1{plus minus}0.26 cm longer (12% more males making the top length grade). Moreover, there was no effect of irradiated feed on fur quality. Irradiation of mink feed with subsequent frozen storage of the meat component improved the microbiological quality by decreasing the incidence of Pseudomonas sp. and Salmonella sp. Radiation pasteurization of mink feed (frozen meat to 1 kGy, and dry feed to 2 kGy or more) should therefore help improve feed utilization, keep animals healthier, and reproducing better without affecting fur quality. (author).

  5. Studies on the pathogenesis of Aleutian disease of mink

    International Nuclear Information System (INIS)

    Mueller-Peddinghaus, R.; Meyer zu Schwabedissen, H.; Kalden, J.R.; Trautwein, G.; Ueberschaer, S.

    1980-01-01

    Aleutian disease (AD) of mink most closely resembles systemic lupus erythematosus (SLE) in man; both are immune complex disease. In experimental AD serum immune complexes are determined by the 125 J-C 1 q-binding test using human C 1 q. Mink (n = 12) infected intraperitoneally with Aleutian disease virus (ADV), grown in fetal mink kidney cells, developed during the course of infection a mean of 125 I-C 1 q serum binding equivalent to 3.62 +- 1.68 mg./ml. aggr. HGG. (aggregated human immunoglobulin). Sera of mink (n = 8) which were infected with ADV grown in L-cells showed a less marked 125 I-C 1 q binding with a mean equivalent to 2.52 +- 1.43 mg./ml. aggr. HGG. In contrast control animals (n = 8) treated with non-ADV-infected mink epidermal fibroblasts or Eagle's minimal essential medium substituted with fetal calf serum only bound 125 I-C 1 q equivalent to 1.02 +- 0.99 mg./ml. aggr. HGG. In mink infected with ADV propagated in fetal mink kidney cells a constant increase in the 125 I-C 1 q serum binding occurred from the 4th to the 7th and 13th week after ADV infection. Mink which were infected with ADV propagated in mouse L-cells exhibited a different pattern of the 125 I-C 1 q serum binding capacity with a sharp increase from the 4th to the 7th week, followed by a decline towards the 13th week post infection. The serum 125 I-C 1 q binding capacity of all experimental animal groups exhibited at different times of the experiment a significant correlation with the presence of hypergammaglobulinaemia and raised ADV-antibody titers. From the data obtained it appears that the 125 I-C 1 q binding test, utilizing human C 1 q, is a suitable method for the detection of circulating serum immune complexes in mink during the course of ADV-infection. (orig.) [de

  6. Experimental Pseudomonas aeruginosa mediated rhino sinusitis in mink

    DEFF Research Database (Denmark)

    Kirkeby, S.; Hammer, A. S.; Høiby, N.

    2017-01-01

    The nasal and sinus cavities in children may serve as reservoirs for microorganisms that cause recurrent and chronic lung infections. This study evaluates whether the mink can be used as an animal model for studying Pseudomonas aeruginosa mediated rhino-sinusitis since there is no suitable...... in the infected mink shows features of carbohydrate expression comparable to what has been described in the respiratory system after Pseudomonas aeruginosa infection in humans. It is suggested that the mink is suitable for studying Pseudomonas aeruginosa mediated rhino-sinusitis....

  7. Mural endocarditis caused by Corynebacterium mustelae in a dog with a VSD.

    Science.gov (United States)

    Winter, Randolph L; Gordon, Sonya G; Zhang, Shuping; Hariu, Crystal D; Miller, Matthew W

    2014-01-01

    A 6 yr old female spayed large Munsterlander was evaluated following a 3 wk history of lethargy, inappetence, intermittent fever, and a recent change to the timing of her previously diagnosed heart murmur. Physical examination revealed marked dehydration, lethargy, and a grade 5/6 to-and-fro heart murmur that was auscultated best at the right sternal border. The dog was febrile, and echocardiography revealed a large, mobile, vegetative lesion in the right ventricular outflow tract associated with a ventricular septal defect (VSD). Mild aortic insufficiency was present. Corynebacterium mustelae (C. mustelae) was isolated from a pooled blood culture. Treatment of infective endocarditis (IE) was initiated along with supportive care, and the patient was discharged 9 days later. The dog remained without clinical signs 132 days after discharge. VSD is rarely mentioned as a predisposing factor for development of IE in veterinary literature; however, this report highlights that dogs with a VSD may be at risk for IE. To the authors' knowledge, this is the first documented case of a canine infection with C. mustelae. Infection with C. mustelae in this case represents a novel agent for IE in the dog.

  8. Coccidia infections in Danish farmed mink

    DEFF Research Database (Denmark)

    Petersen, Heidi Huus; Hansen, Mette Sif; Chriél, Mariann

    day (April-October 2016) from bitches and cups on 30 farms (n=335 mink) from South- or North Jutland, or Zealand. Minimum once, 60.9% (n=204) mink excreted Eimeria, 56.7% (n=190) Isospora and 20.9% (n=70) excreted both coccidia. Positive mink were identified on all farms. Eimeria prevalence was higher...... on the Zealand farms (25.4±2.2%, PEimeria prevalence peaked in June-July (12.6%-24.9%), while Isospora prevalence peaked in July-August (12.......1%-27.6%). More cups (19.5%) than bitches (4.6%) were Isospora positive, while Eimeria prevalence was similar for cups (15.7%) and bitches (10.5%). For cups, Eimeria prevalence peaked when cups were 7-11 weeks old and again when 18-24 weeks old. Isospora prevalence peaked in cups 13-15 weeks old. Three Eimeria...

  9. Natural and experimental Helicobacter mustelae reinfection following successful antimicrobial eradication in ferrets.

    Science.gov (United States)

    Batchelder, M; Fox, J G; Hayward, A; Yan, L; Shames, B; Murphy, J C; Palley, L

    1996-03-01

    Recrudescence or reinfection may occur after eradication of Helicobacter pylori in humans. We used the ferret Helicobacter mustelae model to investigate the effect of prior infection and eradication on reinfection by experimental and natural routes. Two groups of ferrets with naturally acquired H. mustelae infection were treated with an eradication protocol using amoxicillin, metronidazole, and bismuth subsalicylate. The ferrets were monitored for recrudescence by repeated cultures of endoscopic gastric mucosal biopsies. The ferrets were challenged at 17 months (group I) and 6 months (group II) after eradication with a strain of H. mustelae having a distinctive restriction endonuclease analysis pattern. The eradication protocol was repeated to eliminate the infection produced by experimental challenge. The ferrets were then cohoused intermittently with naturally infected ferrets. The original H. mustelae infection was successfully eliminated by the eradication protocol. No recrudescence was observed in group I for 12 months nor for 3 months in group II after eradication. All ferrets became persistently reinfected with the challenge strain. The infection from the challenge strain was eradicated successfully. No ferrets in group I and all ferrets in group II became infected through cohousing. These results suggest that though prior infection with H. mustelae may confer some protection against reinfection, such protection is not universal in all circumstances; that susceptibility to reinfection by contact with infected animals varies between individuals; and that age may be a factor in this individual variability. These results are applicable to studies of reinfection after eradication of H. pylori in humans.

  10. Can spectroscopy in combination with chemometrics replace minks in digestibility tests?

    DEFF Research Database (Denmark)

    Dahl, P.L.; Christensen, B.M.; Munck, L.

    2000-01-01

    One of the most relevant but expensive methods of assessing the quality of fish meal is the physiological digestibility test with minks. The purpose of this study was to determine whether spectroscopic and chemical analyses evaluated with chemometrics can replace minks in digestibility tests....... The spectroscopic methods used were the two complementary techniques of fluorescence emission and near-infrared reflectance. The investigation included 54 samples of high-quality fish meal ranging from 89.6 to 93.9 on the mink digestibility index. The investigation also included determination of seven quality...... parameters in the fish meal to substantiate the spectroscopic models on the mink digestibility. These quality parameters include the content of protein, oil, water, water-soluble protein, ash and the biogenic substance cadaverine as well as the titration value. The study demonstrates that the mink...

  11. Molecular characterization of the Himalayan mink

    DEFF Research Database (Denmark)

    Benkel, Bernhard F.; Rouvinen-Watt, Kirsti; Farid, Hossain

    2009-01-01

    A rare color variant of the American mink (Neovison vison), discovered on a ranch in Nova Scotia and referred to as the "marbled" variety, carries a distinctive pigment distribution pattern resembling that found in some other species, e.g., the Siamese cat and the Himalayan mouse. We tested...... the hypothesis that the color pattern in question-light-colored body with dark-colored points (ears, face, tail, and feet)-is due to a mutation in the melanin-producing enzyme tyrosinase (TYR) that results in temperature-sensitive pigment production. Our study shows that marbled mink carry a mutation in exon 4...... of the TYR gene (c.1835C > G) which results in an amino acid substitution (p.H420Q). The location of this substitution corresponds to the amino acid position that is also mutated in the TYR protein of the Himalayan mouse. Thus, the marbled variant is more aptly referred to as the Himalayan mink....

  12. Energy metabolism and nutrient oxidation in the pregnant mink (Mustela vison) as a model for other carnivores.

    Science.gov (United States)

    Tauson, A H; Elnif, J; Hansen, N E

    1994-12-01

    The mink is a strict carnivore and a seasonal breeder, which may be used as an experimental model for other carnivores. The present investigation comprised a total of 44 balance experiments, each including a 24-h measurement of heat production by indirect calorimetry, carried out from mating until close to parturition. For observations with a nonprotein respiratory quotient between 0.7 and 1.0 (n = 42), quantitative oxidation of nutrients was calculated. The weight gain of the uterus during pregnancy was studied in 41 females killed either before mating, before implantation, after implantation or in mid or late true gestation, and energy retention was calculated. Heat production did not increase with advancing stage of gestation. Mean energy retention was low and in some individuals with repeated measurements even negative, indicating that part of the energy requirement for pregnancy may be supplied by mobilization of body reserves. This was reflected by a high level (42%) of fat oxidation in relation to total heat production. Protein oxidation accounted for 38% of heat production. The weight gain of the uterus during pregnancy could be described by logarithmic functions. Energy deposition in fetal tissue was low and only averaged approximately 350 kJ 47 d after mating.

  13. Sequencing and characterization of mixed function monooxygenase genes CYP1A1 and CYP1A2 of Mink (Mustela vison) to facilitate study of dioxin-like compounds

    International Nuclear Information System (INIS)

    Zhang Xiaowei; Moore, Jeremy N.; Newsted, John L.; Hecker, Markus; Zwiernik, Matthew J.; Jones, Paul D.; Bursian, Steven J.

    2009-01-01

    As part of an ongoing effort to understand aryl hydrocarbon receptor (AhR) mediated toxicity in mink, cDNAs encoding for CYP1A1 and the CYP1A2 mixed function monooxygenases were cloned and characterized. In addition, the effects of selected dibenzofurans on the expression of these genes and the presence of their respective proteins (P4501A) were investigated, and then correlated with the catalytic activities of these proteins as measured by ethoxyresorufin O-deethylase (EROD) and methoxyresorufin O-deethylase (MROD) activities. The predicted protein sequences for CYP1A1 and CYP1A2 comprise 517 and 512 amino acid residues, respectively. The phylogenetic analysis of the mink CYP1As with protein sequences of other mammals revealed high sequence homology with sea otter, seals and the dog, with amino acid identities ranging from 89 to 95% for CYP1A1 and 81 to 93% for CYP1A2. Since exposure to both 2,3,7,8-Tetrachlorodibenzofuran (TCDF) and 2,3,4,7,8-Pentachlorodibenzofuran (PeCDF) resulted in dose-dependent increases of CYP1A1 mRNA, CYP1A2 mRNA and CYP1A protein levels an underlying AhR-mediated mechanism is suggested. The up-regulation of CYP1A mRNA in liver was more consistent to the sum adipose TEQ concentration than to the liver TEQ concentration in minks treated with TCDF or PeCDF. The result suggested that the hepatic-sequestered fraction of PeCDF was biologically inactive to the induction of CYP1A1 and CYP1A2

  14. Animal prevalence of livestock-associated methicillin-resistant Staphylococcus aureus infive Danish mink (Neovison vison) farms

    DEFF Research Database (Denmark)

    Fertner, Mette Ely; Pedersen, Karl; Hansen, Julie Elvekjær

    /50) of screened healthy Danish mink farms were found positive. LA-MRSA in mink is believed to originate from contaminated slaughter-offal in the mink feed. Objective. The objective of the present study was to identify the animal-prevalence of LA-MRSA in five Danish minkfarms. Materials and Methods. We collected 1......,500 mink carcasses from five Danish mink farms. Farmers were asked to collect 100 mink for each of the three consecutive months following the whelping period (May-July 2017). From each carcass, the right forepaw and a pharyngeal-swab was collected for investigation of MRSA by enrichment, followed...... may be explained by an overall low animal-prevalence in the farm. Another explanation could be the high proportion of young mink kits (15/20) tested. All mink kits were

  15. Evaluation of the choline status in mink fed different levels and sources of choline

    DEFF Research Database (Denmark)

    Hedemann, Mette Skou; Damgaard, Birthe Marie; Clausen, T.N.

    2012-01-01

    Choline is an essential nutrient but the daily need for choline in mink has never been determined. Two experiments were performed to evalutate the choline status in mink kits and full-grown mink fed different levels of choline. In the first experiment mink kits were fed a synthetic diet with chol...

  16. Application of qPCR assays for diagnosing causes of viral mink diarrhea. Preliminary results

    DEFF Research Database (Denmark)

    Hartby, Christina Marie; Kvisgaard, Lise Kirstine; Larsen, Lars Erik

    to diarrhea in mink. Rotavirus is poorly described in mink, but has previously been demonstrated in feces from mink pups with and without clinical signs (Jorgensen et al. 1996). The pathogenicity of these viruses could be related to viral load, virulence and the age of the mink. Therefore, there is a need...

  17. Bacterial protein meal in diets for pigs and minks

    DEFF Research Database (Denmark)

    Hellwing, Anne Louise Frydendahl; Tauson, Anne-Helene; Skrede, Anders

    2007-01-01

    The effect of increasing the dietary content of bacterial protein meal (BPM) on protein turnover rate, and on nucleic acid and creatinine metabolism in growing minks and pigs was investigated in two experiments. In each experiment, 16 animals were allocated to four experimental diets. The diets...... containing no BPM served as controls, i.e. for minks diet M1, for pigs P1; the experimental diets contained increasing levels of BPM to replace fish meal (minks) or soybean meal (pigs), so that up to 17% (P2), 20% (M2), 35% (P3), 40% (M3), 52% (P4), and 60% (M4) of digestible N was BPM derived. Protein...... turnover rate was measured by means of the end-product method using [15N]glycine as tracer and urinary nitrogen as end-product. In minks, protein flux, synthesis, and breakdown increased significantly with increasing dietary BPM. In pigs, diet had no observed effect on protein turnover rate. The intake...

  18. Promotion of ulcerative duodenitis in young ferrets by oral immunization with Helicobacter mustelae and muramyl dipeptide.

    Science.gov (United States)

    Whary, M T; Palley, L S; Batchelder, M; Murphy, J C; Yan, L; Taylor, N S; Fox, J G

    1997-06-01

    The purpose of this study was to determine whether oral immunization of ferret kits with a whole-cell sonicate of Helicobacter mustelae lysate (Hml) and the adjuvant muramyl dipeptide (MDP) would reduce the incidence of natural colonization with H. mustelae and the extent of Helicobacter-associated gastritis by enhancing the host mucosal immune response. Between the ages of 4 and 11 weeks, 44 ferret kits were gavaged with Hml and various doses of MDP. The extent of gastritis and duodenitis and the immune response to H. mustelae were evaluated. All kits became colonized naturally with H. mustelae and the majority developed mild to severe gastritis and duodenitis. Kits that received Hml with MDP developed significantly greater inflammation of the gastric antrum and duodenum, as compared to kits vaccinated with Hml alone. Vaccination with Hml and 50 micrograms of MDP was associated with severe lesions in the proximal duodenum characterized by accumulation of mononuclear inflammatory cells, mucosal erosion, and ulceration. Although serum antibody specific for H. mustelae in 4-week-old kits was approximately 50% of adult levels, a finding attributable to passively acquired maternal antibody, both systemic and mucosal antibody levels became depressed over time despite oral vaccination. The humoral immune response was sufficiently low to prevent detection of any significant dose effect of MDP on antibody levels among experimental groups. Oral vaccination of young ferrets with Hml and 50 micrograms MDP increased the risk of Helicobacter-associated mucosal ulceration in the proximal duodenum, which was associated with low humoral (but significant cell-mediated) immune responses to H. mustelae. In retrospect, the frequency of vaccination may have suppressed the systemic humoral immune response, thereby promoting mucosal damage by H. mustelae. The 50-microgram dose of MDP enhanced the cell-mediated immune response, which indirectly contributed to development of severe lesions

  19. Carl L. Estes Lake. Sabine River, Texas. Draft Environmental Statement.

    Science.gov (United States)

    1975-10-01

    Didelphis marsupialis), raccoon (Procyon lotor), mink ( Mustela vison ), nutria, and various rodents. The variety of bird species is fairly large...has another bearing on the welfare of fish in that they contribute significantly to the cycles of nutrients and respiratory gases in the ecosystem. 2.39

  20. Identifying QTL and genetic correlations between fur quality traits in mink (Neovison vison)

    DEFF Research Database (Denmark)

    Thirstrup, Janne Pia; Anistoroaei, Razvan Marian; Guldbrandtsen, Bernt

    2014-01-01

    Mapping of QTL affecting fur quality traits (guard hair length, guard hair thickness, density of wool, surface of the fur and quality) and skin length was performed in a three-generation mink population (F2 design). In the parental generation, Nordic Brown mink were crossed reciprocally with Amer......Mapping of QTL affecting fur quality traits (guard hair length, guard hair thickness, density of wool, surface of the fur and quality) and skin length was performed in a three-generation mink population (F2 design). In the parental generation, Nordic Brown mink were crossed reciprocally...... with American Black short nap mink. In all, 1082 mink encompassing three generations were used for the analyses. The mink were genotyped for 104 microsatellites covering all 14 autosomes. The QTL analyses were performed by least-square regression implemented in gridqtl software. Genetic and phenotypic...

  1. Antimicrobial resistance among pathogenic bacteria from mink (Neovison vison) in Denmark

    DEFF Research Database (Denmark)

    Nikolaisen, Nanett Kvist; Lassen, Desireé Corvera Kløve; Chriél, Mariann

    2017-01-01

    of antimicrobial resistance among pathogenic bacteria isolated from Danish mink during the period 2014-2016. The aim of this investigation was to provide data on antimicrobial resistance and consumption, to serve as background knowledge for new veterinary guidelines for prudent and optimal antimicrobial usage...... and macrolides. Conclusions: The study showed that antimicrobial resistance was common in most pathogenic bacteria from mink, in particular hemolytic E. coli. There is a need of guidelines for prudent use of antimicrobials for mink....

  2. Infection studies in mink with seal-derived morbillivirus

    DEFF Research Database (Denmark)

    Blixenkrone-Møller, M; Svansson, V; Have, P.

    1989-01-01

    Morbillivirus derived from diseased harbour seals (Phoca vitulina) has characteristics of acute virulent canine distemper virus infection in mink. The infection induced a disease resembling the acute systemic and nervous form of canine distemper.......Morbillivirus derived from diseased harbour seals (Phoca vitulina) has characteristics of acute virulent canine distemper virus infection in mink. The infection induced a disease resembling the acute systemic and nervous form of canine distemper....

  3. Pre-slaughter bodyweight and daily gains in mink are independent ...

    African Journals Online (AJOL)

    Natasza

    2017-03-13

    Mar 13, 2017 ... larger space and low density of animals might result in increased use of .... Heritability of body length and weight in mink, and the effects of time of birth and litter ... slaughterhouse offal and supplementary folic acid in mink diet.

  4. Morphology investigation of the mink’s brain (Mustela vison

    Directory of Open Access Journals (Sweden)

    Milanović Valentina

    2013-01-01

    Full Text Available The mink is a strict carnivore and a seasonal breeder, which may be used as an experimental model for other carnivores. Using anatomical methods, 32 brains of the N. American mink were examined. It was found that the brain consists of four ventricles. Also, it was noted that the posterior horn was missing and that the olfactory recess was present in the lateral ventricle, a large-size interthalamic connection was present in the third ventricle, and a flat, necklace like bottom in the fourth ventricle. Only recently, the ins and outs of the mink’s anatomical structure have begun to absorb the attention of anatomists. Apparently, it is related to the fact that fury animals, among them the mink, are being domesticated. For this reason and because of easy access to the material, the purpose of brain dissection is to familiarize with the three dimensional structure of the brain and teach one of the great methods of studying the brain: looking at its structure.

  5. Acoustic Ecology and Remote Acoustic Monitoring of a Minke Whale Population

    National Research Council Canada - National Science Library

    Gedamke, Jason

    2000-01-01

    Sound is the most effective means of communication in the ocean. A uniquely inquisitive minke whale population on the northern Great Barrier Reef presents an unprecedented research opportunity to study minke acoustics...

  6. Remedial Investigation for Cold Regions Research and Engineering Laboratory (CRREL) Hanover, New Hampshire. Phase 2. Volume 1. Sections 1.0 Through 8.0. Revision 2

    Science.gov (United States)

    1994-03-18

    banks, wide-ranging carnivorous mammals also might occur locally, such as the Mink { Mustela vison ). 6.2.3.6 Rare and Endangered Species In the...from direct contact with soil, sediment, and/or surface water Respiratory intake of contaminants from surface water via gills of fish and/or

  7. The American mink (Neovison vison) is a competent host for native European parasites.

    Science.gov (United States)

    Martínez-Rondán, F J; Ruiz de Ybáñez, M R; Tizzani, P; López-Beceiro, A M; Fidalgo, L E; Martínez-Carrasco, C

    2017-11-30

    The American mink (Neovison vison) is a mustelid native to North America that was introduced in Europe and the former USSR for fur farming. Throughout the last century, accidental or deliberate escapes of mink from farms caused the establishment of stable feral populations. In fact, the American mink is considered an invasive alien species in 28 European countries. The present study evaluates the gastrointestinal and cardiopulmonary helminth fauna of the American mink in Galicia (NW Spain) to understand its role as a potential reservoir for parasites affecting other autochthonous mustelids. In the period 2008-2014, fifty American mink (35 males and 15 females) of different ages (22 immature and 28 adults) from the provinces of Lugo, Ourense and Pontevedra were captured and sacrificed. Eight parasite species were found (6 nematodes and 2 trematodes) with the following prevalences: Molineus patens (68%), Aonchotheca putorii (54%), Crenosoma melesi (10%), Aonchotheca annulosa (8%), Angiostrongylus daskalovi (6%), Aelurostrongylus spp. (2%), Troglotrema acutum (2%) and an unidentified trematode (2%). Eighty-two per cent of the mink harboured helminths, including 15 animals (30%) infected by only one parasite species, 19 (38%) by two species, 5 (10%) by three species and 2 mink (4%) by four species. All helminth species identified are native to European mustelids. Statistical models were used to evaluate if animal characteristics (age, sex and weight), date and capture area influenced the prevalence, intensity or parasite richness. Statistical differences were detected only in models for intensity of M. patens, A. putorii and C. melesi. This is the first report of Angiostrongylus daskalovi, a cardiopulmonary nematode, and A. annulosa, a gastrointestinal nematode specific of rodents, in American mink. Moreover, although the fluke T. acutum has already been cited in American mink, to our knowledge, the present study represents the first report of this trematode in the

  8. Development of vocalization and hearing in American mink (Neovison vison)

    DEFF Research Database (Denmark)

    Brandt, Christian; Malmkvist, Jens; Nielsen, Rasmus L.

    2013-01-01

    -tested (P=0.004). Large numbers of mink are kept in fur industry farms, and our results are important to the understanding of sound communication, which is part of their natural behaviour. Our results also suggest mink as an interesting model for studying the development of mammalian hearing and its......American mink (Neovison vison) kits are born altricial and fully dependent on maternal care, for which the kits’ vocalizations appear essential. We used auditory brainstem responses (ABRs) to determine: (1) hearing sensitivity of adult females from two breeding lines known to differ in maternal...... behaviour and (2) development of hearing in kits 8–52days of age. We also studied sound production in 20 kits throughout postnatal days 1 to 44. Adult female mink had a broad hearing range from 1kHz to above 70kHz, with peak sensitivity (threshold of 20dB SPL) at 8–10kHz, and no difference...

  9. Responses of mink to auditory stimuli: Prerequisites for applying the ‘cognitive bias’ approach

    DEFF Research Database (Denmark)

    Svendsen, Pernille Maj; Malmkvist, Jens; Halekoh, Ulrich

    2012-01-01

    The aim of the study was to determine and validate prerequisites for applying a cognitive (judgement) bias approach to assessing welfare in farmed mink (Neovison vison). We investigated discrimination ability and associative learning ability using auditory cues. The mink (n = 15 females) were...... farmed mink in a judgement bias approach would thus appear to be feasible. However several specific issues are to be considered in order to successfully adapt a cognitive bias approach to mink, and these are discussed....

  10. Environmentally enriched male mink gain more copulations than stereotypic, barren-reared competitors.

    Directory of Open Access Journals (Sweden)

    María Díez-León

    Full Text Available Wild carnivores in zoos, conservation breeding centres, and farms commonly live in relatively small, unstimulating enclosures. Under these captive conditions, in a range of species including giant pandas, black-footed ferrets, and European mink, male reproductive abilities are often poor. Such problems have long been hypothesized to be caused by these animals' housing conditions. We show for the first time that rearing under welfare-improving (i.e., highly valued and stress-reducing environmental enrichments enhances male carnivores' copulatory performance: in mate choice competitions, enriched male American mink (Neovison vison mated more often than non-enriched males. We screened for several potential mediators of this effect. First was physiological stress and its impact on reproductive physiology; second, stress-mediated changes in morphology and variables related to immunocompetence that could influence male attractiveness; and third, behavioural changes likely to affect social competence, particularly autistic-like excessive routine and repetition ('perseveration' as is reflected in the stereotypies common in captive animals. Consistent with physiological stress, excreted steroid metabolites revealed that non-enriched males had higher cortisol levels and lower androgen levels than enriched conspecifics. Their os penises (bacula also tended to be less developed. Consistent with reduced attractiveness, non-enriched males were lighter, with comparatively small spleens and a trend to greater fluctuating asymmetry. Consistent with impaired social competence, non-enriched males performed more stereotypic behaviour (e.g., pacing in their home cages. Of all these effects, the only significant predictor of copulation number was stereotypy (a trend suggesting that low bodyweights may also be influential: highly stereotypic males gained the fewest copulations. The neurophysiological changes underlying stereotypy thus handicap males sexually. We

  11. Chronic fuel oil toxicity in American mink (Mustela vison): systemic and hematological effects of ingestion of a low-concentration of bunker C fuel oil

    International Nuclear Information System (INIS)

    Schwartz, Julie A.; Aldridge, Brian M.; Lasley, Bill L.; Snyder, Paul W.; Stott, Jeff L.; Mohr, F. Charles

    2004-01-01

    Petroleum oil enters the coastal marine environment through various sources; marine mammals such as sea otters that inhabit this environment may be exposed to low concentrations of petroleum hydrocarbons through ingestion of contaminated prey. The inability to perform controlled studies in free-ranging animals hinders investigations of the effects of chronic petroleum oil exposure on sea otter morbidity and mortality, necessitating the development of a reliable laboratory model. We examined the effects of oral exposure to 500 ppm bunker C fuel oil over 113-118 days on American mink, a species phylogenetically related to the sea otter. Hematological parameters and organs were examined for fuel oil-associated changes. Hepatic cytochrome P4501A1 mRNA expression and fecal cortisol concentrations were also measured. Ingestion of fuel oil was associated with a decrease in erythrocyte count, hemoglobin concentration (Hgb), hematocrit (HCT), and an increase in mean corpuscular volume (MCV). Total leukocytes were elevated in the fuel oil group from increases in neutrophils, lymphocytes, and monocytes. Significant interactions between fuel oil and antigen challenge were found for erythrocyte parameters, monocyte and lymphocyte counts. Liver and adrenal weights were increased although mesenteric lymph node weights were decreased in the fuel oil group. Hepatic cytochrome P4501A1 mRNA was elevated in the fuel oil group. Fecal cortisol concentration did not vary between the two groups. Our findings show that fuel oil exposure alters circulating leukocyte numbers, erythrocyte homeostasis, hepatic metabolism and adrenal physiology and establish a framework to use mink as a model for sea otters in studying the systemic effects of marine contaminants

  12. Comparison of canine parvovirus with mink enteritis virus by restriction site mapping.

    OpenAIRE

    McMaster, G K; Tratschin, J D; Siegl, G

    1981-01-01

    The genomes of canine parvovirus and mink enteritis virus were compared by restriction enzyme analysis of their replicative-form DNAs. Of 79 mapped sites, 68, or 86%, were found to be common for both types of DNA, indicating that canine parvovirus and mink enteritis virus are closely related viruses. Whether they evolved from a common precursor or whether canine parvovirus is derived from mink enteritis virus, however, cannot be deduced from our present data.

  13. Recombinant proteins as vaccines for protection against disease induced by infection with mink astrovirus

    DEFF Research Database (Denmark)

    2012-01-01

    and polypeptides of the capsid protein of a novel mink astrovirus strain denoted DK7627. Such polynucleotides and polypeptides may be used for the production of vaccines against mink astrovirus which may induce pre-weaning diarrhoea in minks. The invention furthermore relates to vectors, host cells, compositions...

  14. Genetic variation of the MHC class II DRB genes in the Japanese weasel, Mustela itatsi, endemic to Japan, compared with the Siberian weasel, Mustela sibirica.

    Science.gov (United States)

    Nishita, Y; Abramov, A V; Kosintsev, P A; Lin, L-K; Watanabe, S; Yamazaki, K; Kaneko, Y; Masuda, R

    2015-12-01

    Major histocompatibility complex (MHC) genes encode proteins that play a critical role in vertebrate immune system and are highly polymorphic. To further understand the molecular evolution of the MHC genes, we compared MHC class II DRB genes between the Japanese weasel (Mustela itatsi), a species endemic to Japan, and the Siberian weasel (Mustela sibirica), a closely related species on the continent. We sequenced a 242-bp region of DRB exon 2, which encodes antigen-binding sites (ABS), and found 24 alleles from 31 M. itatsi individuals and 17 alleles from 21 M. sibirica individuals, including broadly distributed, species-specific and/or geographically restricted alleles. Our results suggest that pathogen-driven balancing selection have acted to maintain the diversity in the DRB genes. For predicted ABS, nonsynonymous substitutions exceeded synonymous substitutions, also indicating positive selection, which was not seen at non-ABS. In a Bayesian phylogenetic tree, two M. sibirica DRB alleles were basal to the rest of the sequences from mustelid species and may represent ancestral alleles. Trans-species polymorphism was evident between many mustelid DRB alleles, especially between M. itatsi and M. sibirica. These two Mustela species divided about 1.7 million years ago, but still share many MHC alleles, indicative of their close phylogenetic relationship. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  15. Environmental enrichment reduces signs of boredom in caged mink.

    Directory of Open Access Journals (Sweden)

    Rebecca K Meagher

    Full Text Available Animals housed in impoverished cages are often labelled 'bored'. They have also been called 'apathetic' or 'depressed', particularly when profoundly inactive. However, these terms are rarely operationally defined and validated. As a negative state caused by under-stimulation, boredom should increase interest in stimuli of all kinds. Apathy (lack of interest, by contrast, should manifest as decreased interest in all stimuli, while anhedonia (loss of pleasure, a depressive symptom should specifically decrease interest in normally rewarding stimuli. We tested the hypotheses that mink, a model carnivore, experience more boredom, depression-like apathy, or anhedonia in non-enriched (NE cages than in complex, enriched (E cages. We exposed 29 subjects (13 E, 16 NE to ten stimuli categorized a priori as aversive (e.g. air puffs, rewarding (e.g. evoking chasing or ambiguous/neutral (e.g. candles. Interest in stimuli was assessed via latencies to contact, contact durations, and durations oriented to stimuli. NE mink contacted all stimuli faster (P = 0.003 than E mink, and spent longer oriented to/in contact with them, albeit only significantly so for ambiguous ones (treatment*type P<0.013. With stimulus category removed from statistical models, interest in all stimuli was consistently higher among NE mink (P<0.0001 for all measures. NE mink also consumed more food rewards (P = 0.037. Finally, we investigated whether lying down while awake and stereotypic behaviour (both increased by NE housing predicted these responses. Lying awake positively co-varied with certain measures of increased exploration. In contrast, stereotypic 'scrabbling' or locomotion (e.g. pacing did not. Overall, NE mink showed no evidence of apathy or depression, but instead a heightened investigation of diverse stimuli consistent with boredom. This state was potentially indicated by spending much time lying still but awake (although this result requires replication. Boredom can

  16. Toxaphene in minke whales (Balaenoptera acutorostrata) from the North Atlantic

    International Nuclear Information System (INIS)

    Gouteux, B.; Muir, D.C.G.; Backus, S.; Born, E.W.; Dietz, R.; Haug, T.; Metcalfe, T.; Metcalfe, C.; Oien, N.

    2008-01-01

    Toxaphene contamination of minke whales (Balaenoptera acutorostrata) from North Atlantic waters was examined for the first time. Total toxaphene and ΣCHB (sum of 11 chlorobornanes) concentrations in blubber samples ranged from 170 ± 110 and 41 ± 39 ng/g lipid weight (l.w.) for female minke whales from southeastern Greenland to 5800 ± 4100 and 1100 ± 780 ng/g l.w. for males from the North Sea, respectively. Very large variations in toxaphene concentrations among sampling areas were observed suggesting a spatial segregation of minke whales. However, much of the apparent geographical discrimination was explained by the seasonal fluctuation of animal fat mass. Patterns of CHBs in males revealed that recalcitrant CHBs were in higher proportions in animals from the more easterly areas than in animals from the more westerly areas. This trend may be influenced by the predominance of the US, over the European, input of toxaphene to North Atlantic waters. - High levels of toxaphene were found in different sub-populations of minke whales from North Atlantic waters

  17. Toxaphene in minke whales (Balaenoptera acutorostrata) from the North Atlantic

    Energy Technology Data Exchange (ETDEWEB)

    Gouteux, B. [Aquatic Ecosystem Protection Research Division, Environment Canada, Burlington, ON, L7R 4A6 (Canada); Muir, D.C.G. [Aquatic Ecosystem Protection Research Division, Environment Canada, Burlington, ON, L7R 4A6 (Canada)], E-mail: Derek.Muir@ec.gc.ca; Backus, S. [Aquatic Ecosystem Protection Research Division, Environment Canada, Burlington, ON, L7R 4A6 (Canada); Born, E.W. [Greenland Institute of Natural Resources, P.O. Box 570, DK-3900 Nuuk, Greenland (Denmark); Dietz, R. [National Environmental Research Institute, Department of Arctic Environment, Frederiksborgvej 399, P.O. Box 358, DK-4000 Roskilde (Denmark); Haug, T. [Institute of Marine Research, P.O. Box 6404, N-9294 Tromso (Norway); Metcalfe, T.; Metcalfe, C. [Worsfold Water Quality Centre, Trent University, Peterborough, ON, K9J 7B8 (Canada); Oien, N. [Institute of Marine Research, P.O. Box 1870, N-5817 Bergen (Norway)

    2008-05-15

    Toxaphene contamination of minke whales (Balaenoptera acutorostrata) from North Atlantic waters was examined for the first time. Total toxaphene and {sigma}CHB (sum of 11 chlorobornanes) concentrations in blubber samples ranged from 170 {+-} 110 and 41 {+-} 39 ng/g lipid weight (l.w.) for female minke whales from southeastern Greenland to 5800 {+-} 4100 and 1100 {+-} 780 ng/g l.w. for males from the North Sea, respectively. Very large variations in toxaphene concentrations among sampling areas were observed suggesting a spatial segregation of minke whales. However, much of the apparent geographical discrimination was explained by the seasonal fluctuation of animal fat mass. Patterns of CHBs in males revealed that recalcitrant CHBs were in higher proportions in animals from the more easterly areas than in animals from the more westerly areas. This trend may be influenced by the predominance of the US, over the European, input of toxaphene to North Atlantic waters. - High levels of toxaphene were found in different sub-populations of minke whales from North Atlantic waters.

  18. Bite marks in mink—Induced experimentally and as reflection of aggressive encounters between mink

    DEFF Research Database (Denmark)

    Hansen, Steffen W; Møller, Steen Henrik; Damgaard, Birthe Marie

    2014-01-01

    of the brown colour type (N = 140) and the white colour type (N = 60). Twentybrown and 20 white mink (housed in pairs since weaning) were housed individually at theage of 16 weeks. Every second week (at the age of 20, 22, 24, 25 and 28 weeks), four brownand four white mink were subjected to pressure......: (1) experimentally applied pressure on the skin can be recog-nized as bite marks in brown mink at pelting, (2) bite marks are easier to detect on brownmink than on white coloured mink (P ...tFor many years, bite marks have been used as an indicator for aggression in mink productionsystems. However, the validity of bite marks as indicator of aggression has recently beenquestioned. We therefore tested the following hypotheses: (1) experimentally applied pressure to, or penetration of...

  19. Molecular identification of Taenia mustelae cysts in subterranean rodent plateau zokors (Eospalax baileyi).

    Science.gov (United States)

    Zhao, Fang; Ma, Jun-Ying; Cai, Hui-Xia; Su, Jian-Ping; Hou, Zhi-Bin; Zhang, Tong-Zuo; Lin, Gong-Hua

    2014-07-01

    Cestode larvae spend one phase of their two-phase life cycle in the viscera of rodents, but cases of cestodes infecting subterranean rodents have only been rarely observed. To experimentally gain some insight into this phenomenon, we captured approximately 300 plateau zokors (Eospalax baileyi), a typical subterranean rodent inhabiting the Qinghai-Tibet Plateau, and examined their livers for the presence of cysts. Totally, we collected five cysts, and using a mitochondrial gene (cox1) and two nuclear genes (pepck and pold) as genetic markers, we were able to analyze the taxonomy of the cysts. Both the maximum likelihood and Bayesian methods showed that the cysts share a monophyly with Taenia mustelae, while Kimura 2-parameter distances and number of different sites between our sequences and T. mustelae were far less than those found between the examined sequences and other Taeniidae species. These results, alongside supporting paraffin section histology, imply that the cysts found in plateau zokors can be regarded as larvae of T. mustelae, illustrating that zokors are a newly discovered intermediate host record of this parasite.

  20. On-farm biosecurity practices and causes of preweaning mortality in Canadian commercial mink kits.

    Science.gov (United States)

    Compo, Nicole; Pearl, David L; Tapscott, Brian; Storer, Amanda; Hammermueller, Jutta; Brash, Marina; Turner, Patricia V

    2017-09-08

    Mink are an important animal commodity group in Canada and excessive kit mortality represents a significant loss to production. National biosecurity standards have been developed for Canadian mink farms, but it is unclear how well these standards have been implemented as there are no studies correlating management practices of mink producers with causes of death in mink kits. To that end, we surveyed Ontario mink producers on their biosecurity and management practices and conducted almost 5660 post mortem examinations on found-dead, preweaned kits to characterize mink farm biosecurity practices and causes of death in preweaned kits. We found that very few biosecurity and management practices were uniformly used by producers, despite good awareness of appropriate practices. Use of personal protective equipment was implemented by fewer than 50% of respondents, while control of mink shed access, disinfection of feed containers after use, and use of a rodent control program were the only practices implemented by greater than 70% of respondents. Only 18% of producers reported regular use of antimicrobials in feed or water, although 91% stated they used antimicrobials for treatment of bacterial diseases on a regular basis. On post mortem examination, no gross abnormalities were noted in 71% of the kits, 45% were thought to be stillborn or aborted, 27% had some form of abnormal fluid distribution in the body, and 2% had a congenital malformation. A subset of 69 gastrointestinal tract samples was submitted for bacterial culture, of which 45 samples yielded sufficient growth. Most interesting was the identification of Salmonella enterica serovar Heidelberg in 11% of samples. The results of this study will provide a benchmark for Canadian mink producers and their veterinarians, defining the areas to which greater attention should be given to ensure more rigorous biosecurity practices are in place. Ultimately, these improvements in practices may contribute to increased mink

  1. Acute interstitial pneumonia in mink kits inoculated with defined isolates of Aleutian mink disease parvovirus

    DEFF Research Database (Denmark)

    Alexandersen, Søren; Larsen, S; Aasted, B

    1994-01-01

    of low virulence. The 107 mink kits that survived inoculation with ADV as newborns developed lesions typical of classical Aleutian disease irrespective of the ADV isolate used. The lesions consisted of chronic immune complex-mediated glomerulonephritis and infiltrations with mononuclear cells, including...

  2. The first draft reference genome of the American mink ( Neovison vison )

    DEFF Research Database (Denmark)

    Cai, Zexi; Petersen, Bent; Sahana, Goutam

    2017-01-01

    The American mink (Neovison vison) is a semiaquatic species of mustelid native to North America. It’s an important animal for the fur industry. Many efforts have been made to locate genes influencing fur quality and color, but this search has been impeded by the lack of a reference genome. Here we...... present the first draft genome of mink. In our study, two mink individuals were sequenced by Illumina sequencing with 797 Gb sequence generated. Assembly yielded 7,175 scaffolds with an N50 of 6.3 Mb and length of 2.4 Gb including gaps. Repeat sequences constitute around 31% of the genome, which is lower...

  3. Are Antarctic minke whales unusually abundant because of 20th century whaling?

    Science.gov (United States)

    Ruegg, Kristen C; Anderson, Eric C; Scott Baker, C; Vant, Murdoch; Jackson, Jennifer A; Palumbi, Stephen R

    2010-01-01

    Severe declines in megafauna worldwide illuminate the role of top predators in ecosystem structure. In the Antarctic, the Krill Surplus Hypothesis posits that the killing of more than 2 million large whales led to competitive release for smaller krill-eating species like the Antarctic minke whale. If true, the current size of the Antarctic minke whale population may be unusually high as an indirect result of whaling. Here, we estimate the long-term population size of the Antarctic minke whale prior to whaling by sequencing 11 nuclear genetic markers from 52 modern samples purchased in Japanese meat markets. We use coalescent simulations to explore the potential influence of population substructure and find that even though our samples are drawn from a limited geographic area, our estimate reflects ocean-wide genetic diversity. Using Bayesian estimates of the mutation rate and coalescent-based analyses of genetic diversity across loci, we calculate the long-term population size of the Antarctic minke whale to be 670,000 individuals (95% confidence interval: 374,000-1,150,000). Our estimate of long-term abundance is similar to, or greater than, contemporary abundance estimates, suggesting that managing Antarctic ecosystems under the assumption that Antarctic minke whales are unusually abundant is not warranted.

  4. Installation Restoration Program. Phase 2 - Confirmation/ Quantification. Stage 2 for McChord Air Force Base, Washington

    Science.gov (United States)

    1986-04-30

    canadensis), beaver (Castor canadensis), muskrat (Ondatra zibethica), and mink ( Mustela vison ). Mule or blacktail deer (Oedocoileus hemionus), raccoon...aprons, blouse, trousers) C. Respiratory protection using half-facepiece air purifying res- pirator with replaceable filter cartridges 1...Hierarchy of protective controls: engineered, admini- strative, work practice, personal protective clothing and eq- ^.pment. J-6 2. Forms of respiratory

  5. Modelling growth of five different colour types of mink | Liu | South ...

    African Journals Online (AJOL)

    The objective of this study was to estimate and compare the growth curve parameters for live weight of standard black, brown, mahogany, Hedlund white and sapphire minks. The data were collected from five colour types in the period from seven days to 24 weeks of age. Three hundred mink (about 60 of each colour types) ...

  6. Molecular characterization and phylogenetic analysis of Babesia sp. NV-1 detected from wild American Mink ( Neovison vison ) in Hokkaido, Japan.

    Science.gov (United States)

    Hirata, Haruyuki; Ishinabe, Satoki; Jinnai, Michio; Asakawa, Mitsuhiko; Ishihara, Chiaki

    2013-04-01

    Babesiosis is a tick-borne protozoan disease affecting many mammalian species worldwide, caused by the intraerythrocytic multiplication of Babesia spp. The present study aimed to detect the presence of Babesia sp. in 13 American mink from Hokkaido, Japan. One of 13 animals was positive, as indicated by nested PCR targeting the 18S ribosomal RNA (SSU rDNA) and subunit 7 (eta) of the chaperonin-containing t-complex polypeptide 1 (CCT7) genes from species of Babesia and Theileria. Sequencing of the PCR product of SSU rDNA revealed 99% homology to the isolates of Babesia sp. SAP#131 found in raccoons in Hokkaido, whereas that of the CCT7 gene showed 80% homology to the isolates of Babesia gibsoni in dogs as determined by BLAST analysis. We refer to the cognate sequence as Babesia sp. NV-1. Phylogenetic analyses of SSU rDNA and CCT7 genes from Babesia sp. NV-1 revealed them to be most closely related to the Babesia sp. SAP#131 from a raccoon in Hokkaido and to canine B. gibsoni, respectively. Here, we provide the first molecular evidence of the Babesia sp. NV-1 parasite in feral American mink ( Neovison vison ) in Hokkaido, Japan.

  7. Mortality in farmed mink: Systematic collection versus arbitrary submissions for diagnostic investigation

    DEFF Research Database (Denmark)

    Rattenborg, Erik; Dietz, Hans-Henrik; Andersen, T.H.

    1999-01-01

    The distribution of diagnoses of mortality in mink submitted to the Danish Veterinary Laboratory (DVL) for diagnostic investigation in the calendar year 1997 was compared with the diagnoses of mortality in all dead mink collected at 4 selected farms (project farms) during the same period. A total...

  8. Does removal of mammalian predators significantly affect success of simulated nests in linear habitats? Case study on American mink Mustela vison \\& Predation on simulated duck nests in relation to nest density and habitat type

    OpenAIRE

    PADYŠÁKOVÁ, Eliška

    2007-01-01

    This thesis is made up of two studies dealing with predation of waterfowl nests. in the first study, we determined wheather removal of introduced predator Mustela vison affected nest survival of simulated duck nests in linear habitat. In the second study, we tested two hypothesis: 1)predation depends on density of waterfowl nests, 2)mammals are main predators in forest habitat and birds mainly depredate nests deployed in open land.

  9. Morfologisk og molekylær karakterisering af en eimeriaart fra danske mink

    DEFF Research Database (Denmark)

    Petersen, Heidi Huus; Chriél, Mariann; Hansen, Mette Sif

    2017-01-01

    I perioden april til oktober 2016, blev fæces fra mink (Neovison vison) på 30 danske minkgårde undersøgt med henblik på at fastlægge forekomsten og arterne af Eimeria hos danske mink (Neovison vison). På basis af morfologisk undersøgelse af sporulerede oocyster, blev oocyster som lignede tidligere...... beskrevet art, kaldet E. vison identificeret. Den fundne art var dog en smule mindre end E. vison. Efterfølgende blev oocysterne forsøgt identificeret molekylært og sammenlignet med sekvenser i Genbank. Desværre var der ikke tilgængelige mink sekvenser i GenBank, og den nyligt fundne Eimeria art kaldes...... derfor for Eimeria vison-like. I alt fandtes 2,6% (108/4.141) af de underøgte fæcesprøver positive for E. vison-like oocyster ved mikroskopi, svarende til at 23,9% (78 mink) af minkene udskilte E. vison-like oocyster mindst én gang i undersøgelsesperioden....

  10. Serum amyloid A protein (SAA) from mink, horse, and man: a comparative study

    International Nuclear Information System (INIS)

    Marhaug, G.; Husby, G.; Husebeck, A.; Sletten, K.

    1986-01-01

    Serum amyloid A protein (SAA) was isolated from mink, horse, and human serum by ultracentrifugation and gel filtration and characterized by two-dimensional gel electrophoresis, Western blotting followed by autoradiography and N-terminal amino acid analysis. SAA was found in similar quantities in the high density lipoprotein (HDL) fraction of serum from a patient suffering from systemic juvenile rheumatoid arthritis (JRA) and mink stimulated with lipopolysaccharide (LPS), and in somewhat smaller quantities in serum from horses stimulated with Escherichia coli cultures. Only very small quantities were present in normal human controls and not detectable in normal mink and horse. Striking similarities were found between human and mink SAA with respect to molecular weight, isolectric point and degree of heterogeneity, while the molecular weight, isolectric point and degree of heterogeneity, while the molecular weight of horse SAA seemed to be somewhat lower, and no obvious heterogeneity could be demonstrated in this protein using two-dimensional gel electrophoresis. Immunologic cross-reactivity between SAA from the three species was not found. In contrast to human and horse HDL, mink HDL was found not to contain apoA-II and only minute amounts of apoC proteins. Normal horse HDL also contained additional apoproteins not present in HDL from the other species. N-terminal amino acids analysis of SAA from mink and horse demonstrated the same similarity with the corresponding AA protein as previously reported for human SAA/AA

  11. Site fidelity of female minke whales in the Gulf of. St Lawrence, Canada

    NARCIS (Netherlands)

    Moreira Lopes, Xenia; Bérubé, Martine; Berrow, Simon D.; Larsen, Finn; Haug, Tore; Hoekendijk, Jeroen; Hoelzel, A. Rus; Kot, Brian; Øien, Nils; Oosting, Tom; Pampoulie, Christophe; Ramp, Christian; Robbins, Jooke; Ryan, Conor; Sears, Richard; Simon, Malene; Skaug, H. J.; Verkuil, Yvonne; Vikingsson, G.A.; Witting, Lars; van der Zee, Jurjan; Palsboll, Per

    2017-01-01

    The minke whale (Balaenoptera acutorostrata) is a globally-distributed species whose population ecology is poorly understood. Most knowledge about minke whale populations, including gender differences, originates from historical data collected during commercial harvests. Here we present results from

  12. Lymphotropism and host responses during acute wild-type canine distemper virus infections in a highly susceptible natural host

    DEFF Research Database (Denmark)

    Nielsen, Line; Søgaard, Mette; Jensen, Trine Hammer

    2009-01-01

    The mechanisms behind the in vivo virulence of immunosuppressive wild-type Morbillivirus infections are still not fully understood. To investigate lymphotropism and host responses we have selected the natural host model of canine distemper virus (CDV) infection in mink. This model displays...

  13. [Introduction of species and microevolution: the European beaver, raccoon dog, and American mink].

    Science.gov (United States)

    Korablev, N P; Korablev, M P; Korablev, P N

    2011-01-01

    Nine skull samples of the beaver Castor fiber, six samples of the raccoon dog Nyctereutes procyonoides, and six samples of the American mink Neovison vison were studied using phenetic and craniometric methods. Analysis of the phenofund structure suggests that in all of the studied species the emergence of novel character variations does not lead to their fixation with a significant frequency. Considerable morphological variability emerges in the contact zone of different autochtonous populations, of wild and breeding forms, as well as in geographically and reproductively isolated small groups of individuals. Morphological differences of introduced animals fit into the conception of species polymorphism and are smoothed over when separate colonies merge into metapopulations, which does not lead to the emergence of novel stable taxa.

  14. Consequences of outbreaks of influenza A virus in farmed mink (Neovison vison) in Denmark in 2009 and 2010

    DEFF Research Database (Denmark)

    Chriél, Mariann; Jensen, Trine Hammer; Hjulsager, Charlotte Kristiane

    2012-01-01

    Influenza in mink (Neovison vison) is assumed to be rare, but outbreaks have previously been reported in farmed mink. The first report was from Swedish mink farms in 1984 which was caused by influenza A virus H10N4 of avian origin. In 2009 and 2010 outbreaks of respiratory disease were seen...... in several Danish mink farms. In all of the farms, the clinical symptoms were upper respiratory tract symptoms with sneezing and coughing as the most dominant symptoms. Peracute deaths were seen in mink without any clinical symptoms. Influenza H3N2 was found detected by PCR in the lungs from diseased mink...... and four of these farms used feed medication in three weeks. The farmers, however, noted that the medication had little or no effect. The most plausible way of transmission of the influenza is from the raw untreated pig waste containing lungs used in the production of mink feed. Because the first clinical...

  15. Consequences of outbreaks of influenza A virus in farmed mink (Neovison vison) in Denmark in 2009 and 2010

    DEFF Research Database (Denmark)

    Chriél, Mariann; Jensen, Trine Hammer; Hjulsager, Charlotte Kristiane

    2012-01-01

    and four of these farms used feed medication in three weeks. The farmers, however, noted that the medication had little or no effect. The most plausible way of transmission of the influenza is from the raw untreated pig waste containing lungs used in the production of mink feed. Because the first clinical......Influenza in mink (Neovison vison) is assumed to be rare, but outbreaks have previously been reported in farmed mink. The first report was from Swedish mink farms in 1984 which was caused by influenza A virus H10N4 of avian origin. In 2009 and 2010 outbreaks of respiratory disease were seen...... in several Danish mink farms. In all of the farms, the clinical symptoms were upper respiratory tract symptoms with sneezing and coughing as the most dominant symptoms. Peracute deaths were seen in mink without any clinical symptoms. Influenza H3N2 was found detected by PCR in the lungs from diseased mink...

  16. Effects of group size on behaviour, growth and occurrence of bite marks in farmed mink

    DEFF Research Database (Denmark)

    Axelsson, Helene M. K.; Hansen, Steffen W.; Loberg, Jenny

    2017-01-01

    The aims of this study were to investigate the occurrence of stereotypic behaviours and the activity level in farmed mink when group housed in climbing cages and if group housing increase aggression by assessing the prevalence of bite marks. This was studied in juvenile mink of the colour types...... sunset during six periods of five days each from August-October. After pelting, the leather side of the undried skins were visually inspected for bite marks. Stereotypic behaviours were infrequent (0.1% of observations). Pair housed mink in climbing cages were more "inactive out in cage" than pair housed...... mink in standard cages (p 0.0001), but cage type had no effect on the behaviours "being in nest box", "active out in cage", "interactions with enrichments" or "social interactions" (n.s.). Group sizes of three or four mink increased the behaviours "active out in cage" (P 0.0001) and decreased "being...

  17. Pulse of inflammatory proteins in the pregnant uterus of European polecats (Mustela putorius) leading to the time of implantation.

    Science.gov (United States)

    Lindeberg, Heli; Burchmore, Richard J S; Kennedy, Malcolm W

    2017-03-01

    Uterine secretory proteins protect the uterus and conceptuses against infection, facilitate implantation, control cellular damage resulting from implantation, and supply pre-implantation embryos with nutrients. Unlike in humans, the early conceptus of the European polecat ( Mustela putorius ; ferret) grows and develops free in the uterus until implanting at about 12 days after mating. We found that the proteins appearing in polecat uteri changed dramatically with time leading to implantation. Several of these proteins have also been found in pregnant uteri of other eutherian mammals. However, we found a combination of two increasingly abundant proteins that have not been recorded before in pre-placentation uteri. First, the broad-spectrum proteinase inhibitor α 2 -macroglobulin rose to dominate the protein profile by the time of implantation. Its functions may be to limit damage caused by the release of proteinases during implantation or infection, and to control other processes around sites of implantation. Second, lipocalin-1 (also known as tear lipocalin) also increased substantially in concentration. This protein has not previously been recorded as a uterine secretion in pregnancy in any species. If polecat lipocalin-1 has similar biological properties to that of humans, then it may have a combined function in antimicrobial protection and transporting or scavenging lipids. The changes in the uterine secretory protein repertoire of European polecats is therefore unusual, and may be representative of pre-placentation supportive uterine secretions in mustelids (otters, weasels, badgers, mink, wolverines) in general.

  18. An extended anchored linkage map and virtual mapping for the american mink genome based on homology to human and dog

    DEFF Research Database (Denmark)

    Anistoroaei, Razvan Marian; Ansari, S.; Farid, A.

    2009-01-01

    hybridization (FISH) and/or by means of human/dog/mink comparative homology. The average interval between markers is 8.5 cM and the linkage groups collectively span 1340 cM. In addition, 217 and 275 mink microsatellites have been placed on human and dog genomes, respectively. In conjunction with the existing...... comparative human/dog/mink data, these assignments represent useful virtual maps for the American mink genome. Comparison of the current human/dog assembled sequential map with the existing Zoo-FISH-based human/dog/mink maps helped to refine the human/dog/mink comparative map. Furthermore, comparison...... of the human and dog genome assemblies revealed a number of large synteny blocks, some of which are corroborated by data from the mink linkage map....

  19. Energy metabolism in young mink kits (Neovison vison) affected by protein and carbohydrate level in the diet

    DEFF Research Database (Denmark)

    Hellwing, Anne Louise Frydendahl; Hansen, Niels Enggaard; Tauson, Anne-Helene

    2010-01-01

    The mink is a strict carnivore and mink diets usually have a high content of protein. The energy metabolism in young minks in the transition period from milk to solid food is not investigated in detail, and the protein requirement is poorly defined. The substrate oxidation can give useful...

  20. Oral Malignant Melanoma in a Ferret ( Mustela putorius furo).

    Science.gov (United States)

    d'Ovidio, Dario; Rossi, Giacomo; Meomartino, Leonardo

    2016-06-01

    Oral malignant melanomas are one of the most common oral malignant neoplasms in dogs but are rare in other domesticated species. This case report describes the clinical manifestations and histological appearance of oral melanoma in a ferret ( Mustela putorius furo). To the authors' knowledge, this is the first published description of a clinical case and histopathological findings of oral melanoma in this species.

  1. Chromosomal mapping of canine-derived BAC clones to the red fox and American mink genomes.

    Science.gov (United States)

    Kukekova, Anna V; Vorobieva, Nadegda V; Beklemisheva, Violetta R; Johnson, Jennifer L; Temnykh, Svetlana V; Yudkin, Dmitry V; Trut, Lyudmila N; Andre, Catherine; Galibert, Francis; Aguirre, Gustavo D; Acland, Gregory M; Graphodatsky, Alexander S

    2009-01-01

    High-quality sequencing of the dog (Canis lupus familiaris) genome has enabled enormous progress in genetic mapping of canine phenotypic variation. The red fox (Vulpes vulpes), another canid species, also exhibits a wide range of variation in coat color, morphology, and behavior. Although the fox genome has not yet been sequenced, canine genomic resources have been used to construct a meiotic linkage map of the red fox genome and begin genetic mapping in foxes. However, a more detailed gene-specific comparative map between the dog and fox genomes is required to establish gene order within homologous regions of dog and fox chromosomes and to refine breakpoints between homologous chromosomes of the 2 species. In the current study, we tested whether canine-derived gene-containing bacterial artificial chromosome (BAC) clones can be routinely used to build a gene-specific map of the red fox genome. Forty canine BAC clones were mapped to the red fox genome by fluorescence in situ hybridization (FISH). Each clone was uniquely assigned to a single fox chromosome, and the locations of 38 clones agreed with cytogenetic predictions. These results clearly demonstrate the utility of FISH mapping for construction of a whole-genome gene-specific map of the red fox. The further possibility of using canine BAC clones to map genes in the American mink (Mustela vison) genome was also explored. Much lower success was obtained for this more distantly related farm-bred species, although a few BAC clones were mapped to the predicted chromosomal locations.

  2. Albinism in the american mink (Neovison vison) is associated with a tyrosinase nonsense mutation

    DEFF Research Database (Denmark)

    Anistoroaei, Razvan Marian; Fredholm, Merete; Christensen, Knud

    2008-01-01

    Albino phenotypes are documented in various species including the American mink. In other species the albino phenotypes are associated with tyrosinase (TYR) gene mutations; therefore TYR was considered the candidate gene for albinism in mink. Four microsatellite markers were chosen in the prodicted...

  3. Phenozan Influence on the Physiological-Biochemical Parameters of the Young Minks Leading to Their Advanced Properties

    Directory of Open Access Journals (Sweden)

    Nikolai A. Balakirev

    2016-01-01

    Full Text Available The antioxidant influence on the physiological-biochemical parameters of productive animals is one of the most important directions in modern animal science. Phenozan is a synthetic antioxidant with high biological activities including those for animals. The positive effects of phenozan on the physiological-biochemical parameters of the mink blood were found. These effects are leading to the intensive growth, strong health, enhancement of the mink fur quality, and so forth. The total amount of the erythrocytes in the mink blood increased by about 4-5% due to phenozan inclusion in the mink diet. Even a more significant increase was found in hemoglobin content in the mink blood and erythrocytes (by about 9.5% and 8.8%, resp. due to phenozan inclusion of 100 mg/day, whereas it was found only by about 6.1–5.3% or 5.5–0.3%, owing to phenozan inclusion of 50 or 150 mg/day. For many other parameters the optimal phenozan concentration was about 100 mg/day per head according to the result obtained for all studied animal groups. Thus, phenozan can be successfully used as a biologically active compound for mink nutrition and is recommended for other farm animals.

  4. Phylogenetic analysis of the haemagglutinin gene of canine distemper virus strains detected from breeding foxes, raccoon dogs and minks in China.

    Science.gov (United States)

    Zhao, Jian-Jun; Yan, Xi-Jun; Chai, Xiu-Li; Martella, Vito; Luo, Guo-Liang; Zhang, Hai-Ling; Gao, Han; Liu, Ying-Xue; Bai, Xue; Zhang, Lei; Chen, Tao; Xu, Lei; Zhao, Chun-Fei; Wang, Feng-Xue; Shao, Xi-Qun; Wu, Wei; Cheng, Shi-Peng

    2010-01-06

    Canine distemper virus (CDV) infects a variety of carnivores, including wild and domestic Canidae. Genetic/antigenic heterogeneity has been observed among the various CDV strains, notably in the haemagglutinin (H) gene, that appears as a good target to gather epidemiological information. Based on sequence analysis of the H gene, wild-type CDV strains cluster into distinct geographic lineages (genotypes), irrespective of the species of isolation. The sequence of the H gene of 28 CDV strains detected from both vaccinated and non-vaccinated breeding foxes, raccoon dogs and minks from different geographical areas of China during the years 2004-2008 was determined. All the CDV strains but two (strains HL and HLJ2) were characterized as Asia-1 genotype and were highly similar to each other (96.2-99.7% at the amino acid [aa] level) and to other Asia-1 strains (96.1-99.5% aa) previously detected in China. The CDV strains HL and HLJ2 were both collected from foxes in Heilongjiang province in 2005. Strain HL resembled CDVs of the Arctic genotype (GR88-like) and displayed high aa identity (98.0%) to the Chinese canine strain Liu. By converse, strain HLJ2 was barely related to CDVs of the Asia-2 genotype (88.7-90.3% aa identity), and could represent a novel CDV genotype, tentatively proposed as Asia-3. These results suggest that at least three different CDV genotypes, distantly related (81.8-91.6% aa identity) to the vaccine strains, Onderstepoort-like (America-1 genotype), are currently circulating in breeding foxes, raccoon dogs and minks in China, and that the genotype Asia-1 is predominant. Whether the diversity between wild-type CDVs and the vaccine strains may affect, to some extent, the efficacy of the vaccines deserves further investigations.

  5. Passive transfer of antiviral antibodies restricts replication of Aleutian mink disease parvovirus in vivo

    DEFF Research Database (Denmark)

    Alexandersen, Søren; Larsen, S; Cohn, A.

    1989-01-01

    When mink kits were infected neonatally with a highly virulent strain of Aleutian disease virus (ADV), 100% of both Aleutian and non-Aleutian genotype mink died of interstitial pneumonia characterized by permissive ADV infection of alveolar type II cells. Treatment of infected kits with either mi...

  6. Comparison of Pseudomonas aeruginosa isolates from mink by serotyping and pulsed-field gel electrophoresis

    DEFF Research Database (Denmark)

    Hammer, Anne Sofie; Pedersen, Karl; Andersen, Thomas Holmen

    2003-01-01

    Isolates of Pseudomonas aeruginosa from clinical infections in mink were subjected to serotyping and pulsed-field gel electrophoresis (PFGE) using SpeI. A total of 212 isolates of P aeruginosa from the year 1998 to 2001 were included in this study: 168 isolates from mink obtained from 74 farm out...

  7. Parasites of the mink frog (rana septentrionalis) from Minnesota, U.S.A.

    Science.gov (United States)

    Schotthoefer, Anna M.; Bolek, M.G.; Cole, Rebecca A.; Beasley, Val R.

    2009-01-01

    Twenty-two mink frogs, Rana septentrionalis, collected from two locations in Minnesota, United States, were examined for helminth and protozoan blood parasites in July 1999. A total of 16 parasite taxa were recovered including 5 larval digenean trematodes, 7 adult digenean trematodes, 3 nematodes, and I Trypanosorna species. Infracommunities were dominated by the digeneans in terms of richness and abundance. In particular, echinostomatid metacercariae in the kidneys of frogs were the most common parasites found, infecting 100% of the frogs and consisting of about 90% of all helminth individuals recovered. Gorgodera amplicava, Gorgoderina multilohata, Haernaroloechus pan'iplexus, Haernatoloechus breviplexus, Cosnwcercoides dukae, and Oswaldocruzia pipiens represent new host records. The survey presented here represents the second known helminth survey of mink frogs conducted in North America. A summary of metazoan parasites reported from mink frogs is included.

  8. DON-induced changes in bone homeostasis in mink dams

    Directory of Open Access Journals (Sweden)

    Tomaszewska Ewa

    2017-09-01

    Full Text Available Introduction: The aim of the study was to investigate the mechanical and geometric properties as well as bone tissue and mineral density of long bones in mink dams exposed to deoxynivalenol (DON since one day after mating, throughout gestation (ca. 46 d and lactation to pelt harvesting. Material and Methods: Thirty clinically healthy multiparous minks (Neovison vison of the standard dark brown type were used. After the mating, the minks were randomly assigned into two equal groups: nontreated control group and DON group fed wheat contaminated naturally with DON at a concentration of 1.1 mg·kg-1 of feed. Results: The final body weight and weight and length of the femur did not differ between the groups. However, DON contamination decreased mechanical endurance of the femur. Furthermore, DON reduced the mean relative wall thickness and vertical wall thickness of the femur, while vertical cortical index, midshaft volume, and cross-sectional moment of inertia increased. Finally, DON contamination did not alter bone tissue density, bone mineral density, or bone mineral content, but decreased the values of all investigated structural and material properties. Conclusion: DON at applied concentration probably intensified the process of endosteal resorption, which was the main reason for bone wall thinning and the weakening of the whole bone.

  9. Microflora of the gastrointestinal tract of Chernobyl she-minks of different age and immunological state

    International Nuclear Information System (INIS)

    Sudenko, V.I.; Podgorskij, V.S.; Ryasenko, V.I.

    1996-01-01

    The quantitative and species characteristics of bacteria and yeast isolated from the food and gastrointestinal tract of she-minks of different age groups and different immunological state have been established. The species composition of bacteria forming the basic and accompanying microflora of small and large intestine of minks is determined. An increase in the quantity of anaerobic (bifidobacteria), microaerophilic (lactic acid bacilli, enterococci) and aerobic (intestinal bacilli) bacteria in the content of the large intestine of minks has been established in comparison with the stomach and small intestine in all examined groups of animals of various age and immunological state

  10. Blood biochemical parameters in male American mink (Neovison vison before and during the breeding season

    Directory of Open Access Journals (Sweden)

    Bogdan Lasota

    2014-05-01

    Full Text Available This study was aimed at selected blood biochemical parameters in the male American mink before (September-November and during the breeding (January-March season. Blood from 143 Black and Sapphire male mink at one year age was collected. The plasma was assayed for the concentration of glucose, total protein, cholesterol, HDL and LDL fractions, triacylglycerides (TG, and the activity of ALT and AST. Concentrations of glucose, protein, total and HDL/LDL cholesterol, and AST activity were generally slightly higher during the breeding season than during the non-breeding season, but remained within the reference range. In the case of ALT activity and TG concentration, the relations were reversed. The parameters studied in the Sapphire mink showed greater variation, both in- and out of the season. In Black and Sapphire males of the American mink, the studied parameters revealed slightly higher values during the breeding season than the non-breeding season. This will vary depending on the color variety. The decrease in TG concentration during the breeding season may indicate an increased energy demand due to ambient temperature falls, and/or may be a sign of increased energy consumption associated with physical exertion during mating. Consideration should be given to the nutrition of male mink during the breeding season.

  11. Risk factors associated with diarrhea in Danish commercial mink (Neovison vison) during the pre-weaning period

    DEFF Research Database (Denmark)

    Birch, Julie Melsted; Agger, Jens Frederik; Dahlin, Christina

    2017-01-01

    the animals and experienced more females with mastitis compared to farmers on control farms. No significant differences in cleaning practices or hygienic measures between case and control farms were found and there were no differences in drinking water quality, bedding material, composition neither of color......Pre-weaning diarrhea in mink, also known as "sticky kits", is a syndrome and outbreaks occur every year on commercial mink farms in all mink producing countries. Morbidity and mortality can be considerable on a farm with huge economic consequences for the farmer as well as compromised welfare...... for the mink kits. Although efforts have been taken to identify etiologic agents involved in outbreaks, the syndrome is still regarded as multifactorial and recurring problems on the same farms draw attention to management and environmental risk factors. In the pre-weaning period from May to June 2015, a case...

  12. Animal Personality and Mate Preference in American Mink

    DEFF Research Database (Denmark)

    Noer, Christina Lehmkuhl

    are unsustainable. Often, prescribed pairs of animals appear to be incompatible and reproductively unsuccessful. This is also a problem in solitary carnivore species, many of which are threatened with extinction. A suggested solution to this problem is to investigate, which male cues and signals the females use...... or cues: olfactory (urine and faeces), size, and behaviour. The latter may represent aspects of animal personality. The background for this is explained in the introduction (Chapter I). In Chapter II, a published article, I present a novel study on aspects of animal personality in the American mink...... aspects of shyness exist in the farmed American mink. These findings highlight the importance of carefully considering the context as well as the limitations of using the shyness-boldness continuum for describing animal personality traits. Chapter III, a manuscript, adds to the knowledge of animal...

  13. Hemorrhagic pneumonia in mink caused by Pseudomonas aeruginosa

    DEFF Research Database (Denmark)

    Salomonsen, Charlotte Mark

    research has been performed in this field and most published work is more than 25 years old. The studies presented in this thesis aim at elucidating varying aspects of the disease: Article I investigates the relationships of P. aeruginosa isolated from mink hemorrhagic pneumonia using pulsed field gel...... electrophoresis (PFGE) and a commercial typing system based on single nucleotide polymorphisms (SNP) on chosen strains. The results presented in this article show that 70% of P. aeruginosa isolated from outbreaks of hemorrhagic pneumonia in mink consist of unique strains, while the remaining 30% belongs to either...... in hemorrhagic pneumonia caused by P. aeruginosa and E. coli in diagnostic material. The distribution of the two pathogens is visualized using fluorescence in situ hybridization (FISH). Two histological patterns were observed in the work presented in Article II; one was very hemorrhagic with few bacteria while...

  14. Exclusion of candidate genes for coat colour phenotypes of the American mink (Neovison vison)

    DEFF Research Database (Denmark)

    Anistoroaei, Razvan Marian; Markakis, M. N.; Vissenberg, K.

    2012-01-01

    In a previous project, we screened the American mink Bacterial Artificial Chromosome library, CHORI-231, for genes potentially involved in various coat colour phenotypes in the American mink. Subsequently, we 454 sequenced the inserts containing these genes and developed microsatellite markers...... for each of these genes. Here, we describe a lack of association between three different ‘roan-type' phenotypes represented by Cross, Stardust and Cinnamon in American mink and six different genes that we considered to be potentially linked to these phenotypes. Thus, c-KIT (HUGO-approved symbol KIT), ATOH...

  15. USSR Report, Life Sciences Biomedical and Behavioral Sciences.

    Science.gov (United States)

    1987-03-10

    cited involved cloning the DNA of hybrid somatic cells derived from the American mink ( Mustela vison ) and Chinese hamster (X+ cells), carrying a single...was evaluated by the mitochondrial respiratory dynamics as increasing concentrations of cytochrome c were added. The enzymatic activity was...group of rats 48 hr after the landing of Kosmos-605 biological satellite experienced a 40-50% reduction of tissue respiratory rate (AO) and of

  16. Population status, habitat associations, and distribution of the steppe polecat Mustela eversmanii in Europe

    Czech Academy of Sciences Publication Activity Database

    Šálek, Martin; Spassov, N.; Anděra, M.; Enzinger, K.; Ottlecz, B.; Hegyeli, Z.

    2013-01-01

    Roč. 58, č. 3 (2013), s. 233-244 ISSN 0001-7051 Institutional support: RVO:68081766 Keywords : Steppe polecat * Mustela eversmanii * Distribution * Habitat associations * Population status * Europe Subject RIV: EG - Zoology Impact factor: 1.161, year: 2013

  17. Population genetic structure in farm and feral American mink (Neovison vison) inferred from RAD sequencing-generated single nucleotide polymorphisms

    DEFF Research Database (Denmark)

    Thirstrup, Janne Pia; Ruiz-Gonzalez, Aritz; Pujolar, José Martin

    2015-01-01

    Feral American mink populations (Neovison vison), derived from mink farms, are widespread in Europe. In this study we investigated genetic diversity and genetic differentiation between feral and farm mink using a panel of genetic markers (194 SNP) generated from RAD sequencing data. Sampling incl...

  18. Influence of farm, feed-producer and season on incidence of gastrointestinal disorders in Danish farm mink

    DEFF Research Database (Denmark)

    Rattenborg, Erik; Chriél, Mariann; Dietz, Hans H.

    1999-01-01

    The distribution of gastrointestinal disorders in mink in Danish farms is presented based on data collected in a longitudinal design. The time at risk was from weaning in June until pelting in November. The occurrence of gastrointestinal disorders after weaning of the mink kits together with the ...

  19. Estimating population density and connectivity of American mink using spatial capture-recapture.

    Science.gov (United States)

    Fuller, Angela K; Sutherland, Chris S; Royle, J Andrew; Hare, Matthew P

    2016-06-01

    Estimating the abundance or density of populations is fundamental to the conservation and management of species, and as landscapes become more fragmented, maintaining landscape connectivity has become one of the most important challenges for biodiversity conservation. Yet these two issues have never been formally integrated together in a model that simultaneously models abundance while accounting for connectivity of a landscape. We demonstrate an application of using capture-recapture to develop a model of animal density using a least-cost path model for individual encounter probability that accounts for non-Euclidean connectivity in a highly structured network. We utilized scat detection dogs (Canis lupus familiaris) as a means of collecting non-invasive genetic samples of American mink (Neovison vison) individuals and used spatial capture-recapture models (SCR) to gain inferences about mink population density and connectivity. Density of mink was not constant across the landscape, but rather increased with increasing distance from city, town, or village centers, and mink activity was associated with water. The SCR model allowed us to estimate the density and spatial distribution of individuals across a 388 km² area. The model was used to investigate patterns of space usage and to evaluate covariate effects on encounter probabilities, including differences between sexes. This study provides an application of capture-recapture models based on ecological distance, allowing us to directly estimate landscape connectivity. This approach should be widely applicable to provide simultaneous direct estimates of density, space usage, and landscape connectivity for many species.

  20. Estimating population density and connectivity of American mink using spatial capture-recapture

    Science.gov (United States)

    Fuller, Angela K.; Sutherland, Christopher S.; Royle, Andy; Hare, Matthew P.

    2016-01-01

    Estimating the abundance or density of populations is fundamental to the conservation and management of species, and as landscapes become more fragmented, maintaining landscape connectivity has become one of the most important challenges for biodiversity conservation. Yet these two issues have never been formally integrated together in a model that simultaneously models abundance while accounting for connectivity of a landscape. We demonstrate an application of using capture–recapture to develop a model of animal density using a least-cost path model for individual encounter probability that accounts for non-Euclidean connectivity in a highly structured network. We utilized scat detection dogs (Canis lupus familiaris) as a means of collecting non-invasive genetic samples of American mink (Neovison vison) individuals and used spatial capture–recapture models (SCR) to gain inferences about mink population density and connectivity. Density of mink was not constant across the landscape, but rather increased with increasing distance from city, town, or village centers, and mink activity was associated with water. The SCR model allowed us to estimate the density and spatial distribution of individuals across a 388 km2 area. The model was used to investigate patterns of space usage and to evaluate covariate effects on encounter probabilities, including differences between sexes. This study provides an application of capture–recapture models based on ecological distance, allowing us to directly estimate landscape connectivity. This approach should be widely applicable to provide simultaneous direct estimates of density, space usage, and landscape connectivity for many species.

  1. Ecological risk assessment in a large river-reservoir. 8: Experimental study of the effects of polychlorinated biphenyls on reproductive success in mink

    International Nuclear Information System (INIS)

    Halbrook, R.S.; Aulerich, R.J.; Bursian, S.J.; Lewis, L.

    1999-01-01

    As a component of an ecological risk assessment of Poplar Creek (located on the Oak Ridge Reservation [ORR]) and the Clinch River (a large river-reservoir system), fish from Poplar Creek, the Clinch River, and Atlantic Ocean were fed to ranch mink to evaluate reproductive success. Five diets, each composed of 75% fish and 25% normal ranch mink chow, were prepared. Two diets served as reference diets and contained 75% Atlantic Ocean fish or 75% Clinch River fish collected above the ORR. The fish portion of the remaining three diets contained 25, 50, and 75% fish collected from Poplar Creek and 50, 25, and 0% ocean fish, respectively. Five mink groups (eight females and two males each) were each fed one of the prepared diets for 196 days. Polychlorinated biphenyl concentrations were determined in diets and various mink tissues, ethoxyresorufin-O-deethylase (EROD) activity was determined in liver tissue, and reproductive success was evaluated. Concentrations of PCB were greatest in the diet composed of 75% Poplar Creek fish and in tissues from mink fed this diet and their offspring. There was a trend toward decreased adult female and kit weights and reduced mean litter size in mink fed diets containing 75% Poplar Creek fish; however, at 6 weeks of age, kit survival was similar among diet groups. Liver EROD activity significantly increased in adult female mink fed 50 and 75% Poplar Creek fish diets. Estimated dietary concentrations of PCBs were similar to or slightly lower than concentrations associated with adverse effects in experimentally dosed mink. Mercury (Hg) concentrations previously reported in these same mink were below that associated with adverse effects, and there was no indication of additive or synergistic effects from exposure to PCBs plus Hg. It is unlikely that population-level reproductive effects would be observed in mink consuming fish from Poplar Creek on the ORR

  2. A cross-sectional field study on potential associations between feed quality measures and usage of antimicrobials in commercial mink (Neovison vison)

    DEFF Research Database (Denmark)

    Jensen, Vibeke Frøkjær; Sommer, Helle Mølgaard; Struve, Tina

    2017-01-01

    Feed quality is generally assumed to affect health status in animal production. In previous studies, the feed producer has been found to affect the occurrence of gastrointestinal disease and antimicrobial use in Mink (Neovison vison). Mink are fed with moist, freshly produced feed, based...... on perishable ingredients. The objective of this study was to investigate the potential effect of specific feed parameters on antimicrobial use on herd level. The study was cross-sectional, including 1472 mink herds, responsible for 97% of oral antimicrobials prescribed for Danish mink during the study period......, 2012-2014. Data were obtained from the national veterinary prescription database (VetStat), Kopenhagen Fur database, and the Voluntary Feed Control (Mink producers Organization). All feed batches subject to feed control were included. A multi-variable variance analysis was carried out analysing...

  3. Intestinal remodelling in mink fed with reduced protein content

    DEFF Research Database (Denmark)

    Chen, Pengmin; Zhao, Jingbo; Nielsen, Vivi Hunnicke

    2009-01-01

    Low protein intake occurs in humans in relation to diseases, starvation and post-operatively. Low-protein diets may affect the gastrointestinal structure and mechanical function. The aim was to study the passive biomechanical properties and tissue remodelling of the intestine in minks on reduced...... protein diets. Twenty-seven male minks were divided into three groups receiving different protein level in the diet for 6 weeks: High protein level (group H, 55% energy from protein), moderate protein level (group M, 30% energy from protein) and low protein level (group L, 15% energy from protein) (n=9...... groups. Feeding the low-protein diet shifted the stress-strain curves to the right for the circumferential direction, indicating the wall become softer in the circumferential direction. However, no significant difference was observed in the longitudinal direction for any of the intestinal segments...

  4. Brucella ceti Infection in a Common Minke Whale ( Balaenoptera acutorostrata ) with Associated Pathology.

    Science.gov (United States)

    Davison, Nicholas J; Perrett, Lorraine L; Dawson, Claire; Dagleish, Mark P; Haskins, Gary; Muchowski, Jakub; Whatmore, Adrian M

    2017-07-01

    There are three major lineages of marine mammal strains of Brucella spp.: Brucella ceti ST23, found predominantly in porpoises; B. ceti ST26, in pelagic delphinids and ziphiids; and Brucella pinnipedialis ST24/25, predominantly in seals. The isolation of Brucella spp. in mysticetes has been described only in common minke whales ( Balaenoptera acutorostrata ) in Norway and Scotland. We report a third case of Brucella infection and isolation in a minke whale associated with a large abscess. In contrast to the two previous reports that involved isolates of B. pinnipedialis ST24 or the porpoise-associated B. ceti complex ST23, this case was associated with the dolphin-associated B. ceti ST26. Thus, minke whales can be infected naturally with members of all the distinct major lineages of Brucella associated with marine mammals. This report is unique in that the B. ceti ST26 did not originate from a pelagic delphinid or a beaked whale.

  5. Risk factors associated with diarrhea in Danish commercial mink (Neovison vison) during the pre-weaning period.

    Science.gov (United States)

    Birch, Julie Melsted; Agger, Jens Frederik; Dahlin, Christina; Jensen, Vibeke Frøkjær; Hammer, Anne Sofie; Struve, Tina; Jensen, Henrik Elvang

    2017-06-29

    Pre-weaning diarrhea in mink, also known as "sticky kits", is a syndrome and outbreaks occur every year on commercial mink farms in all mink producing countries. Morbidity and mortality can be considerable on a farm with huge economic consequences for the farmer as well as compromised welfare for the mink kits. Although efforts have been taken to identify etiologic agents involved in outbreaks, the syndrome is still regarded as multifactorial and recurring problems on the same farms draw attention to management and environmental risk factors. In the pre-weaning period from May to June 2015, a case control study was carried out on 30 Danish mink farms. Data concerning management, biosecurity, hygiene, feed consumption, antibacterial prescription and production efficiency were analyzed. The proportion of 1-year old females, farm size (total number of females), energy supply per female in the late gestation period, and dogs accessing the farm area were significantly associated with being a case farm. Case farms were prescribed almost twice the amount of antibacterials per gestational unit (female and litter) as in control farms. Farmers on case farms spent significantly more time nursing and treating the animals and experienced more females with mastitis compared to farmers on control farms. No significant differences in cleaning practices or hygienic measures between case and control farms were found and there were no differences in drinking water quality, bedding material, composition neither of color types nor in management regarding litter equalization. Results from this study showed an association between the occurrence of pre-weaning diarrhea on mink farms and parity profile, farm size and feeding intensity in the gestational period. The access of dogs to the farm area was a significant risk factor, but needs further clarification.

  6. Indirect genetic effects contribute substantially to heritable variation in aggression-related traits in group-housed mink (Neovison vison)

    DEFF Research Database (Denmark)

    Alemu, Setegn Worku; Bijma, Peter; Møller, Steen Henrik

    2014-01-01

    Background Since the recommendations on group housing of mink (Neovison vison) were adopted by the Council of Europe in 1999, it has become common in mink production in Europe. Group housing is advantageous from a production perspective, but can lead to aggression between animals and thus raises...... a welfare issue. Bite marks on the animals are an indicator of this aggressive behaviour and thus selection against frequency of bite marks should reduce aggression and improve animal welfare. Bite marks on one individual reflect the aggression of its group members, which means that the number of bite marks...... genetic effects contribute to variation in number of bite marks in group-housed mink. Thus, a genetic selection design that includes both direct genetic and indirect genetic effects could reduce the frequency of bite marks and probably aggression behaviour in group-housed mink....

  7. Additional foraging elements reduce abnormal behaviour – fur-chewing and stereotypic behaviour – in farmed mink (Neovison vison)

    DEFF Research Database (Denmark)

    Malmkvist, Jens; Palme, Rupert; Svendsen, Pernille Maj

    2013-01-01

    We investigated whether provision of additional appetitive and consummatory elements of foraging reduces baseline stress and abnormal behaviour – in terms of fur-chewing and stereotypic behaviour – in farmed mink. We studied 200 juveniles (n = 100 females and 100 males) during the 5-month growth......; (iii) CONS, chunky feed (parts up to 42 mm), replacing conventional feed; (iv) BOTH, access to both biting ropes and chunky feed. In growing mink, biting ropes reduced fur-chewing (P = 0.044) and chunky feed reduced stereotypic behaviour (P = 0.038) and fur-chewing in female mink (P = 0.019). During......, stereotypic behaviour was reduced by provision of chunky feed, increasing the consummatory element in daily foraging. Fur-chewing was reduced upon access to either biting ropes or chunky feed in female mink throughout the study. Our findings support frustrated foraging, mainly consummatory, behind abnormal...

  8. Distribution of Aleutian mink disease virus contamination in the environment of infected mink farms.

    Science.gov (United States)

    Prieto, A; Fernández-Antonio, R; Díaz-Cao, J M; López, G; Díaz, P; Alonso, J M; Morrondo, P; Fernández, G

    2017-05-01

    Control and eradication of Aleutian Mink Disease Virus (AMDV) are a major concern for fur-bearing animal production. Despite notably reducing disease prevalence, current control programs are unable to prevent the reinfection of farms, and environmental AMDV persistence seems to play a major role regarding this issue. In this study 114 samples from different areas and elements of seven infected mink farms were analyzed by qPCR in order to evaluate the environmental distribution of AMDV load. Samples were classified into nine categories, depending on the type of sample and degree of proximity to the animals, the main source of infection. Two different commercial DNA extraction kits were employed in parallel for all samples. qPCR analysis showed 69.3% positive samples with one kit and 81.6% with the other, and significant differences between the two DNA extraction methods were found regarding AMDV DNA recovery. Regarding sample categorization, all categories showed a high percentage of AMDV positive samples (31%-100%). Quantification of positive samples showed a decrease in AMDV load from animal barns to the periphery of the farm. In addition, those elements in direct contact with animals, the street clothes and vehicles of farm workers and personal protective equipment used for sampling showed a high viral load, and statistical analysis revealed significant differences in AMDV load between the first and last categories. These results indicate high environmental contamination of positive farms, which is helpful for future considerations about cleaning and disinfection procedures and biosecurity protocols. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. Evaluation of the zoonotic potential of transmissible mink encephalopathy

    Science.gov (United States)

    Successful transmission of Transmissible Mink Encephalopathy (TME) to cattle supports the bovine hypothesis to the still controversial origin of TME outbreaks. Human and primate susceptibility to classical Bovine Spongiform Encephalopathy (c-BSE) and the transmissibility of L-type BSE to macaques as...

  10. Minkin Aleutian taudin ELISA-testin tuotteistaminen : Insinöörityö

    OpenAIRE

    Poikulainen, Emmi

    2014-01-01

    Aleutian tauti on minkin (Mustela vison) plasmasytoosisairaus. Sen aiheuttaa Aleutian mink disease virus, AMDV, joka on parvovirus. AMDV:n käyttäytyminen on kuitenkin epätyypillistä parvoviruksille. Taudin oireita ovat hypergammaglobulanemia, hengitystieinfektiot ja muut tulehdukset. Tauti on merkittävä, sillä siihen ei ole olemassa käypää hoitoa tai rokotetta, ja se aiheuttaa suuria taloudellisia vahinkoja kaikissa minkin kasvattajamaissa. Ainoa tapa ehkäistä Aleutian taudin tuhoja on ka...

  11. Draft Environmental Assessment for the Combat Support Training Complex, Tyndall Air Force Base, Florida.

    Science.gov (United States)

    1989-07-25

    nwdas Green Seaturtle PK E E 1,19 FISH Acipenser oxyrhynchus &esotoi Gulf Sturgeon SR SSC UR2 17,19 Mustela vison lutensis Florida Mink P - UR2 13...immediately dangerous to life and health (IDLH) with respect to respiratory irritation. This represents the maximum concentration from which one could...Threshold 0.004 mg/m3 ACGIH’ Median effective concentration 1-5 mg/m3 ACGIH Respiratory irritation Permissible exposure limit (PEL) 0.4 mg/m3

  12. Generalised additive models to investigate environmental drivers of Antarctic minke whale (Balaenoptera bonaerensis) spatial density in austral summer

    NARCIS (Netherlands)

    Beekmans, B.W.P.M.; Forcada, J.; Murphy, E.J.; Baar, H.J.W.; Bathmann, U.V.; Fleming, A.H.

    2010-01-01

    There is a need to characterise the physical environment associated with Antarctic minke whale density in order to understand long-term changes in minke whale distribution and density in open waters of the Southern Ocean during austral summer months. To investigate environmental drivers of Antarctic

  13. Comparative Transcriptome Analysis of Mink (Neovison vison) Skin Reveals the Key Genes Involved in the Melanogenesis of Black and White Coat Colour.

    Science.gov (United States)

    Song, Xingchao; Xu, Chao; Liu, Zongyue; Yue, Zhigang; Liu, Linling; Yang, Tongao; Cong, Bo; Yang, Fuhe

    2017-09-29

    Farmed mink (Neovison vison) is one of the most important fur-bearing species worldwide, and coat colour is a crucial qualitative characteristic that contributes to the economic value of the fur. To identify additional genes that may play important roles in coat colour regulation, Illumina/Solexa high-throughput sequencing technology was used to catalogue the global gene expression profiles in mink skin with two different coat colours (black and white). RNA-seq analysis indicated that a total of 12,557 genes were differentially expressed in black versus white minks, with 3,530 genes up-regulated and 9,027 genes down-regulated in black minks. Significant differences were not observed in the expression of MC1R and TYR between the two different coat colours, and the expression of ASIP was not detected in the mink skin of either coat colour. The expression levels of KITLG, LEF1, DCT, TYRP1, PMEL, Myo5a, Rab27a and SLC7A11 were validated by qRT-PCR, and the results were consistent with RNA-seq analysis. This study provides several candidate genes that may be associated with the development of two coat colours in mink skin. These results will expand our understanding of the complex molecular mechanisms underlying skin physiology and melanogenesis in mink and will provide a foundation for future studies.

  14. Spatial distribution of common Minke whale (Balaenoptera acutorostrata) as an indication of a biological hotspot in the East Sea

    Science.gov (United States)

    Lee, Dasom; An, Yong Rock; Park, Kyum Joon; Kim, Hyun Woo; Lee, Dabin; Joo, Hui Tae; Oh, Young Geun; Kim, Su Min; Kang, Chang Keun; Lee, Sang Heon

    2017-09-01

    The minke whale (Balaenoptera acutorostrata) is the most common baleen whale among several marine mammal species observed in Korea. Since a high concentrated condition of prey to whales can be obtained by physical structures, the foraging whale distribution can be an indicator of biological hotspot. Our main objective is verifying the coastal upwelling-southwestern East Sea as a productive biological hotspot based on the geographical distribution of minke whales. Among the cetacean research surveys of the National Institute of Fisheries Science since 1999, 9 years data for the minke whales available in the East Sea were used for this study. The regional primary productivity derived from Moderate-Resolution Imaging Spectroradiometer (MODIS) was used for a proxy of biological productivity. Minke whales observed during the sighting surveys were mostly concentrated in May and found mostly (approximately 70%) in the southwestern coastal areas (whales was found in recent years, which indicate that the major habitats of mink whales have been shifted into the north of the common coastal upwelling regions. This is consistent with the recently reported unprecedented coastal upwelling in the mid-eastern coast of Korea. Based on high phytoplankton productivity and high distribution of minke whales, the southwestern coastal regions can be considered as one of biological hotspots in the East Sea. These regions are important for ecosystem dynamics and the population biology of top marine predators, especially migratory whales and needed to be carefully managed from a resource management perspective.

  15. A comparative, BAC end sequence enabled map of the genome of the American mink (Neovison vison)

    DEFF Research Database (Denmark)

    Benkel, Bernhard F.; Smith, Amanda; Christensen, Knud

    2012-01-01

    In this report we present the results of the analysis of approximately 2.7 Mb of genomic information for the American mink (Neovison vison) derived through BAC end sequencing. Our study, which encompasses approximately 1/1000th of the mink genome, suggests that simple sequence repeats (SSRs...

  16. Canine distemper virus DNA vaccination of mink can overcome interference by maternal antibodies.

    Science.gov (United States)

    Jensen, Trine Hammer; Nielsen, Line; Aasted, Bent; Pertoldi, Cino; Blixenkrone-Møller, Merete

    2015-03-10

    Canine distemper virus (CDV) is highly contagious and can cause severe disease against which conventional live vaccines are ineffective in the presence of maternal antibodies. Vaccination in the presences of maternal antibodies was challenged by vaccination of 5 days old and 3 weeks old mink kits with CDV DNA vaccines. Virus neutralising (VN) antibody responses were induced in mink kits vaccinated with a plasmid encoding the haemaglutinin protein (H) of CDV (n=5, pCDV-H) or a combination of the H, fusion (F) and nucleoprotein (N) of CDV (n=5, pCDV-HFN). These DNA vaccinated kits were protected against virulent experimental infection with field strains of CDV. The pCDV-H was more efficient in inducing protective immunity in the presence of maternal antibodies compared to the pCDV-HFN. The results show that DNA vaccination with the pCDV-H or pCDV-HFN (n=4) only given once at 5 days of age induces virus specific immune response in neonatal mink and protection against virulent CDV exposure later in life. Copyright © 2015 Elsevier Ltd. All rights reserved.

  17. Isolation and characterization of a "phiKMV-like" bacteriophage and its therapeutic effect on mink hemorrhagic pneumonia.

    Directory of Open Access Journals (Sweden)

    Zhenhui Cao

    Full Text Available The objective of this study was to investigate the potential of using phages as a therapy against hemorrhagic pneumonia in mink both in vitro and in vivo. Five Pseudomonas aeruginosa (P. aeruginosa strains were isolated from lungs of mink with suspected hemorrhagic pneumonia and their identity was confirmed by morphological observation and 16S rDNA sequence analysis. Compared to P. aeruginosa strains isolated from mink with hemorrhagic pneumonia in 2002, these isolates were more resistant to antibiotics selected. A lytic phage vB_PaeP_PPA-ABTNL (PPA-ABTNL of the Podoviridae family was isolated from hospital sewage using a P. aeruginosa isolate as host, showing broad host range against P. aeruginosa. A one-step growth curve analysis of PPA-ABTNL revealed eclipse and latent periods of 20 and 35 min, respectively, with a burst size of about 110 PFU per infected cell. Phage PPA-ABTNL significantly reduced the growth of P. aeruginosa isolates in vitro. The genome of PPA-ABTNL was 43,227 bp (62.4% G+C containing 54 open reading frames and lacked regions encoding known virulence factors, integration-related proteins and antibiotic resistance determinants. Genome architecture analysis showed that PPA-ABTNL belonged to the "phiKMV-like Viruses" group. A repeated dose inhalational toxicity study using PPA-ABTNL crude preparation was conducted in mice and no significantly abnormal histological changes, morbidity or mortality were observed. There was no indication of any potential risk associated with using PPA-ABTNL as a therapeutic agent. The results of a curative treatment experiment demonstrated that atomization by ultrasonic treatment could efficiently deliver phage to the lungs of mink and a dose of 10 multiplicity of infection was optimal for treating mink hemorrhagic pneumonia. Our work demonstrated the potential for phage to fight P. aeruginosa involved in mink lung infections when administered by means of ultrasonic nebulization.

  18. Role of hepatic de novo lipogenesis in the development of fasting-induced fatty liver in the American mink (Neovison vison).

    Science.gov (United States)

    Rouvinen-Watt, Kirsti; Harris, Lora; Dick, Morag; Pal, Catherine; Lei, Sha; Mustonen, Anne-Mari; Nieminen, Petteri

    2012-10-28

    American mink (Neovison vison) develop fatty liver quickly in response to food deprivation, which results in preferential mobilisation of n-3 PUFA. The altered n-3:n-6 PUFA ratio in the liver may activate the endocannabinoid system resulting in increased lipid synthesis. The objective of the present study was to investigate the effects of feeding intensity (80 or 120% RDA), dietary fat source (n-3, n-6 or n-9 fatty acids (FA)) and short-term fasting (1-7 d) on hepatic de novo lipogenesis (DNL) and the development of fatty liver in mink. Significantly elevated expression of mRNA encoding for acetyl-CoA carboxylase-1 (ACC-1) and FA synthase (FAS) was observed in the liver of mink fasted for 5-7 d, while upon re-feeding for 28 d after a 7 d food deprivation, DNL returned to pre-fasting levels. The females had a higher expression of ACC-1 and FAS mRNA than the males. In the non-fasted animals, dietary fat source and feeding intensity had significant effects on ACC-1 mRNA. The highest levels were observed in the mink fed the rapeseed oil (n-9) diet at 80% RDA, while the lowest levels were seen when the same diet was fed at 120% RDA. For FAS, the highest gene expression was seen in the fasted mink fed at 80% RDA and the lowest in the non-fasted mink fed at 80%. It is concluded that short-term food deprivation induces hepatic lipidosis in mink and that during this process, hepatic DNL further exacerbates liver fat accumulation.

  19. Influenza A Virus Surveillance in the Invasive American Mink (Neovison vison) from Freshwater Ecosystems, Northern Spain.

    Science.gov (United States)

    Gholipour, H; Busquets, N; Fernández-Aguilar, X; Sánchez, A; Ribas, M P; De Pedro, G; Lizarraga, P; Alarcia-Alejos, O; Temiño, C; Cabezón, O

    2017-08-01

    Influenza A viruses (IAVs) are negative-sense, single-stranded and segmented RNA viruses of the Orthomyxoviridae family that may cause acute respiratory disease in a wide range of birds and mammals. Susceptibility of several species within the family Mustelidae to IAVs has been reported as a result of natural or experimental infections. The objectives of this study were to assess whether free-ranging American mink populations from Northern Spain were infected with IAV and try to define the role of this species in the epidemiology of IAV. Sera from 689 American mink from Northern Spain captured between 2011 and 2014 were tested for the presence of antibodies against IAVs using a commercial competition cELISA. Positive sera were further analysed with haemagglutination inhibition (HI) assay. Fifteen of the 689 (2.2%, 1.3-3.6 CI 95% ) of the American minks analysed were ELISA positive. No significant differences were observed between years of capture, provinces, river basins, sexes or ages of the animals. All seropositive sera resulted negative to the panel strains used in the HI assay, showing that the most relevant strains circulating in swine, the most relevant avian subtypes (H5 and H7) and the H10N4 subtype isolated in minks have not been circulating in this free-ranging exotic carnivore from Spain. In the light of these results, the free-range American mink from Northern Spain do not seem to have an important role in the epidemiology of IAVs. © 2016 Blackwell Verlag GmbH.

  20. Livestock-associated methicillin-resistant Staphylococcus aureus is widespread in farmed mink (Neovison vison)

    DEFF Research Database (Denmark)

    Hansen, Julie Elvekjær; Rhod Larsen, Anders; Skov, Robert Leo

    2017-01-01

    was to examine the presence of LA-MRSA in farmed mink. The investigation comprised three different sample types 1) clinical samples from carcasses submitted to the laboratory for diagnostic examination, 2) paws and pharyngeal swabs from healthy animals collected at pelting, and 3) feed samples from mink feed...... farms, including paw samples (29%) and pharyngeal samples (16%). Twenty out of the 108 feed samples from feed producers were positive for LA-MRSA. The dominant spa-types were t034 and t011 associated to CC398, corresponding to the dominant spa-types detected in the Danish pig production, from which...

  1. Recombinant Newcastle disease viral vector expressing hemagglutinin or fusion of canine distemper virus is safe and immunogenic in minks.

    Science.gov (United States)

    Ge, Jinying; Wang, Xijun; Tian, Meijie; Gao, Yuwei; Wen, Zhiyuan; Yu, Guimei; Zhou, Weiwei; Zu, Shulong; Bu, Zhigao

    2015-05-15

    Canine Distemper Virus (CDV) infects many carnivores and cause several high-mortality disease outbreaks. The current CDV live vaccine cannot be safely used in some exotic species, such as mink and ferret. Here, we generated recombinant lentogenic Newcastle disease virus (NDV) LaSota expressing either envelope glycoproyein, heamagglutinine (H) or fusion protein (F), named as rLa-CDVH and rLa-CDVF, respectively. The feasibility of these recombinant NDVs to serve as live virus-vectored CD vaccine was evaluated in minks. rLa-CDVH induced significant neutralization antibodies (NA) to CDV and provided solid protection against virulent CDV challenge. On the contrast, rLa-CDVF induced much lower NA to CDV and fail to protected mink from virulent CDV challenge. Results suggest that recombinant NDV expressing CDV H is safe and efficient candidate vaccine against CDV in mink, and maybe other host species. Copyright © 2015 Elsevier Ltd. All rights reserved.

  2. Radiation pasteurization of mink feed: Effect of irradiated feed on reproductive performance, growth and fur quality of mink

    Science.gov (United States)

    Passey, C. A.; Roy, D.; Savoie, L.; Malo, R.; Wilson, J.

    No significant differences were observed in the net birth rate of kits/female between the 7 breeding groups. However, there was reduced incidence (P = 0.05) of kit deaths among the females receiving irradiated feed, and larger kit size (P pasteurization of mink feed (frozen meat to 1 kGy, and dry feed to 2 kGy or more) should therefore help improve feed utilization, keep the animals healthier, and reproducing better without affecting fur quality.

  3. Production and characterization of monoclonal antibodies against mink leukocytes

    DEFF Research Database (Denmark)

    Chen, W.S.; Pedersen, Mikael; Gram-Nielsen, S.

    1997-01-01

    Three monoclonal antibodies (mAbs) were generated against mink leukocytes. One antibody reacted with all T lymphocytes, one with all monocytes and one had platelet reactivity. Under reducing conditions, the T lymphocyte reactive antibody immunoprecipitated 18 kDa, 23 kDa, 25 kDa and 32-40 kDa pol...

  4. Doktoritööd : [2007, Anne Lange jt.

    Index Scriptorium Estoniae

    2007-01-01

    20. veebr. kaitseb Anne Lange doktoritööd "The poetics of translation of Ants Oras". 19. jaan. kaitses Tuuli Oder doktoritööd "The model of contemporary professional foreign language teacher". 16. jaan. kaitses Tiit Maran doktoritööd "Conservation biology of the European mink, Mustela lutreola (Linnaeus 1761): decline and causes of extinction". 12. jaan. kaitses Maris Saagpakk doktoritööd "Deutschbaltische Autobiographien als Dokumente des Zeit- und Selbstempfindens: vom Ende des 19. Jh. bis zur Umsiedlung 1939"

  5. Organization of the sleep-related neural systems in the brain of the minke whale (Balaenoptera acutorostrata).

    Science.gov (United States)

    Dell, Leigh-Anne; Karlsson, Karl Ae; Patzke, Nina; Spocter, Muhammad A; Siegel, Jerome M; Manger, Paul R

    2016-07-01

    The current study analyzed the nuclear organization of the neural systems related to the control and regulation of sleep and wake in the basal forebrain, diencephalon, midbrain, and pons of the minke whale, a mysticete cetacean. While odontocete cetaceans sleep in an unusual manner, with unihemispheric slow wave sleep (USWS) and suppressed REM sleep, it is unclear whether the mysticete whales show a similar sleep pattern. Previously, we detailed a range of features in the odontocete brain that appear to be related to odontocete-type sleep, and here present our analysis of these features in the minke whale brain. All neural elements involved in sleep regulation and control found in bihemispheric sleeping mammals and the harbor porpoise were present in the minke whale, with no specific nuclei being absent, and no novel nuclei being present. This qualitative similarity relates to the cholinergic, noradrenergic, serotonergic and orexinergic systems, and the GABAergic elements of these nuclei. Quantitative analysis revealed that the numbers of pontine cholinergic (274,242) and noradrenergic (203,686) neurons, and hypothalamic orexinergic neurons (277,604), are markedly higher than other large-brained bihemispheric sleeping mammals. Small telencephalic commissures (anterior, corpus callosum, and hippocampal), an enlarged posterior commissure, supernumerary pontine cholinergic and noradrenergic cells, and an enlarged peripheral division of the dorsal raphe nuclear complex of the minke whale, all indicate that the suite of neural characteristics thought to be involved in the control of USWS and the suppression of REM in the odontocete cetaceans are present in the minke whale. J. Comp. Neurol. 524:2018-2035, 2016. © 2015 Wiley Periodicals, Inc. © 2015 Wiley Periodicals, Inc.

  6. Structure and Steroidogenesis of the Placenta in the Antarctic Minke Whale (Balaenoptera bonaerensis)

    Science.gov (United States)

    SASAKI, Motoki; AMANO, Yoko; HAYAKAWA, Daisuke; TSUBOTA, Toshio; ISHIKAWA, Hajime; MOGOE, Toshihiro; OHSUMI, Seiji; TETSUKA, Masafumi; MIYAMOTO, Akio; FUKUI, Yutaka; BUDIPITOJO, Teguh; KITAMURA, Nobuo

    2012-01-01

    Abstract There are few reports describing the structure and function of the whale placenta with the advance of pregnancy. In this study, therefore, the placenta and nonpregnant uterus of the Antarctic minke whale were observed morphologically and immunohistochemically. Placentas and nonpregnant uteri were collected from the 15th, 16th and 18th Japanese Whale Research Programme with Special Permit in the Antarctic (JARPA) and 1st JARPA II organized by the Institute of Cetacean Research in Tokyo, Japan. In the macro- and microscopic observations, the placenta of the Antarctic minke whale was a diffuse and epitheliochorial placenta. The chorion was interdigitated to the endometrium by primary, secondary and tertiary villi, which contained no specialized trophoblast cells such as binucleate cells, and the interdigitation became complicated with the progress of gestation. Furthermore, fetal and maternal blood vessels indented deeply into the trophoblast cells and endometrial epithelium respectively with fetal growth. The minke whale placenta showed a fold-like shape as opposed to a finger-like shape. In both nonpregnant and pregnant uteri, many uterine glands were distributed. The uterine glands in the superficial layer of the pregnant endometrium had a wide lumen and large epithelial cells as compared with those in the deep layer. On the other hand, in the nonpregnant endometrium, the uterine glands had a narrower lumen and smaller epithelial cells than in the pregnant endometrium. In immunohistochemical detection, immunoreactivity for P450scc was detected in most trophoblast cells, but not in nonpregnant uteri, suggesting that trophoblast epithelial cells synthesized and secreted the sex steroid hormones and/or their precursors to maintain the pregnancy in the Antarctic minke whale. PMID:23269486

  7. Greenland sharks (Somniosus microcephalus scavenge offal from minke (Balaenoptera acutorostrata whaling operations in Svalbard (Norway

    Directory of Open Access Journals (Sweden)

    Lisa-Marie Leclerc

    2011-06-01

    Full Text Available Minke whale (Balaenoptera acutorostrata tissue (mainly blubber was found in the gastrointestinal tracks of Greenland sharks (Somniosus microcephalus collected in Kongsfjorden, Svalbard, Norway. In order to determine whether the sharks were actively hunting the whales, finding naturally dead whales or consuming offal from whaling, we checked the genetic identity of the whale tissue found in the sharks against the DNA register for minke whales taken in Norwegian whaling operations. All of the minke whale samples from the sharks that had DNA of sufficient quality to perform individual identifications were traceable to the whaling DNA register. During whaling operations, the blubber is stripped from the carcass and thrown overboard. The blubber strips float on the surface and are available for surface-feeding predators. This study revealed that Greenland sharks are scavenging this material; additionally, it demonstrates the capacity of this ‘benthic-feeding’ shark to utilize the whole water column for foraging.

  8. Analysis of Factors Influencing Fur Quality in Minks of Standard, Pastel, Platinum and White Hedlunda Colour Strains

    Directory of Open Access Journals (Sweden)

    Stanisław Socha

    2010-10-01

    Full Text Available The work aimed at the analysis of the factors that influence conformation traits, included animal size and fur quality traits in four colour types of mink: standard, pastel, platinum and white Hedlunda. The data concerns the evaluation of animal conformation traits in the period of three years. The analysis of variance of particular traits indicates statistically significant effect of the year of birth, colour type and animal sex on the majority of analysed traits. Higher means of license evaluation were obtained by males in majority of the traits. Statistic analysis of body weight showed that the highest body weight characterized males of platinum and white Hedlunda colour types. Minks of standard and pastel colour types were characterised by lower body weight. The mean body weight of males was 2581.17g and of females 1401.42g (there is a clear sexual dimorphism in minks. Minks of white Hedlunda colour type were characterised by the highest means of colour purity, both males and females. Other colour types obtained lower means. The best fur quality characterised platinum minks. Variability of traits, measured by variability coefficient, had the highest values in animal weight (in grams and ranged from 6.0 to 32.0%. Variability of total number of scores ranged from 2.00 to 8.20%. Positive phenotypic correlations were the highest between body size (in points and total number of scores (0.676, while the lowest were obtained between body size (in points and fur quality (–0.178.

  9. Comparison of histological lesions in acute hemorrhagic pneumonia in mink associated with Pseudomonas aeruginosa or Escherichia coli

    DEFF Research Database (Denmark)

    Salomonsen, Charlotte Mark; Boye, Mette; Høiby, N.

    2013-01-01

    also occurred in farmed mink. The purpose of this study was to compare histological lesions of acute hemorrhagic pneumonia associated with both P. aeruginosa and E. coli in mink, including a description of tissue distribution of pathogens, in an attempt to differentiate between the 2 disease entities......, as P. aeruginosa was most often found surrounding blood vessels and lining the alveoli, while E. coli showed a more diffuse distribution in the lung tissue. Furthermore, P. aeruginosa often elicited a very hemorrhagic response in the lung, while infection with E. coli was associated with a higher......Hemorrhagic pneumonia can be a major cause of mortality in farmed mink in the fall. In its classic form, hemorrhagic pneumonia is caused by the bacterium Pseudomonas aeruginosa. In recent years, however, outbreaks of this type of pneumonia that are associated with hemolytic Escherichia coli have...

  10. Metabolomic study of plasma from female mink (Neovison vison) with low and high residual feed intake during restrictive and ad libitum feeding

    DEFF Research Database (Denmark)

    Hedemann, Mette Skou; Damgaard, Birthe Marie

    2012-01-01

    was high during restrictive feeding, e.g. betaine, carnitine, and creatine. During ad libitum feeding the plasma level of metabolites that can be characterized as biomarkers of meat intake (creatinine, carnosine, 1- and 3 methylhistidine) was high. The plasma level of lysophosphatidylcholine species......Metabolite profiling may elucidate changes in metabolic pathways under various physiological or nutritional conditions. In the present study two groups of female mink characterized as having a high (16 mink) or low (14 mink) residual feed intake were investigated during restrictive and ad libitum...

  11. Quantitative Analysis of Cellular Proteome Alterations in CDV-Infected Mink Lung Epithelial Cells

    Directory of Open Access Journals (Sweden)

    Mingwei Tong

    2017-12-01

    Full Text Available Canine distemper virus (CDV, a paramyxovirus, causes a severe highly contagious lethal disease in carnivores, such as mink. Mink lung epithelial cells (Mv.1.Lu cells are sensitive to CDV infection and are homologous to the natural host system of mink. The current study analyzed the response of Mv.1.Lu cells to CDV infection by iTRAQ combined with LC–MS/MS. In total, 151 and 369 differentially expressed proteins (DEPs were markedly up-regulated or down-regulated, respectively. Thirteen DEPs were validated via real-time RT-PCR or western blot analysis. Network and KEGG pathway analyses revealed several regulated proteins associated with the NF-κB signaling pathway. Further validation was performed by western blot analysis and immunofluorescence assay, which demonstrated that different CDV strains induced NF-κB P65 phosphorylation and nuclear translocation. Moreover, the results provided interesting information that some identified DEPs possibly associated with the pathogenesis and the immune response upon CDV infection. This study is the first overview of the responses to CDV infection in Mv.1.Lu cells, and the findings will help to analyze further aspects of the molecular mechanisms involved in viral pathogenesis and the immune responses upon CDV infection.

  12. Distance estimation experiment for aerial minke whale surveys

    Directory of Open Access Journals (Sweden)

    Lars Witting

    2009-09-01

    Full Text Available A comparative study between aerial cue–counting and digital photography surveys for minke whales conducted in Faxaflói Bay in September 2003 is used to check the perpendicular distances estimated by the cue-counting observers. The study involved 2 aircraft with the photo plane at 1,700 feet flying above the cue–counting plane at 750 feet. The observer–based distance estimates were calculated from head angles estimated by angle-boards and declination angles estimated by declinometers. These distances were checked against image–based estimates of the perpendicular distance to the same whale. The 2 independent distance estimates were obtained for 21 sightings of minke whale, and there was a good agreement between the 2 types of estimates. The relative absolute deviations between the 2 estimates were on average 23% (se: 6%, with the errors in the observer–based distance estimates resembling that of a log-normal distribution. The linear regression of the observer–based estimates (obs on the image–based estimates (img was Obs=1.1Img (R2=0.85 with an intercept fixed at zero. There was no evidence of a distance estimation bias that could generate a positive bias in the absolute abundance estimated by cue–counting.

  13. Evolutionary analysis of whole-genome sequences confirms inter-farm transmission of Aleutian mink disease virus

    DEFF Research Database (Denmark)

    Hagberg, Emma Elisabeth; Pedersen, Anders Gorm; Larsen, Lars E

    2017-01-01

    Aleutian mink disease virus (AMDV) is a frequently encountered pathogen associated with mink farming. Previous phylogenetic analyses of AMDV have been based on shorter and more conserved parts of the genome, e.g. the partial NS1 gene. Such fragments are suitable for detection but are less useful...... direction of spread. It was however impossible to infer transmission pathways from the partial NS1 gene tree, since all samples from the case farms branched out from a single internal node. A sliding window analysis showed that there were no shorter genomic regions providing the same phylogenetic resolution...

  14. Effects of dietary protein level on nutrients digestibility and reproductive performance of female mink (Neovison vison during gestation

    Directory of Open Access Journals (Sweden)

    Qingkui Jiang

    2015-06-01

    Full Text Available The objective of this study was to determine whether nutrient digestibility and reproductive performance of pregnant mink (Neovison vison were affected by different dietary protein levels. One hundred and twenty female mink were randomly assigned to four groups, receiving diets of fresh material with different protein levels. The dietary protein levels, expressed as percentage of dry matter (DM, were 32, 36, 40 and 44% respectively. These values corresponded to average 320, 360, 400 and 440 g protein/kg DM, respectively. Results were as follows. All of crude protein digestibility, nitrogen (N intake, N retention increased along with dietary protein level increasing. Low protein level (32% significantly reduced the above indicators (P < 0.05. DM digestibility and ether extract digestibility were not affected by dietary protein level. Results of mated females, barren females, kids per litter, live born kids per mated female, birth survival rate, and birth weight showed that mink achieved optimal reproductive performance when dietary protein level was 36%. In conclusion, dietary protein was anticipated to significantly influence some nutrients' utilization. Adopting the appropriate dietary protein level allow better reproduction performance. The most preferable reproductive performance was achieved when diet contained 275.5 g digestible protein per kg DM for female mink in gestation.

  15. Projekt skal undersøge MRSA i danske mink

    DEFF Research Database (Denmark)

    Fertner, Mette Ely; Hansen, Julie Elvekjær; Larsen, Gitte

    2017-01-01

    MRSA blev for første gang isoleret fra to danske mink, som var indsendt til undersøgelse på DTU Veterinærinstituttet, i 2013. I løbet af 2017 gennemfører DTU Veterinærinstituttet et projekt, som har til formål at klarlægge MRSA-forekomsten og smitteoverførslen på danske minkgårde....

  16. Er genomisk selektion en revolution af avlsarbejdet med mink?

    DEFF Research Database (Denmark)

    Berg, Peer

    2011-01-01

    Genomisk selektion udnytter, at den enkelte mink kan genotypes for tusindvis af genetiske markører. Et dyrs avlsværdi kan skønnes med ret stor sikkerhed på basis af en blod- eller hårprøve, hvor dyrets sæt af genetiske markører bestemmes. Det er altså ikke nødvendigt at registrere minkens egne eg...

  17. Filarioid infections in wild carnivores: a multispecies survey in Romania.

    Science.gov (United States)

    Ionică, Angela Monica; Matei, Ioana Adriana; D'Amico, Gianluca; Ababii, Jana; Daskalaki, Aikaterini Alexandra; Sándor, Attila D; Enache, Dorin Valter; Gherman, Călin Mircea; Mihalca, Andrei Daniel

    2017-07-13

    Filarioids are vector-borne parasitic nematodes of vertebrates. In Europe, eight species of filarioids, including zoonotic species, have been reported mainly in domestic dogs, and occasionally in wild carnivores. In Romania, infections with Dirofilaria spp. and Acanthocheilonema reconditum are endemic in domestic dogs. Despite the abundant populations of wild carnivores in the country, their role in the epidemiology of filarioid parasites remains largely unknown. The aim of the present study was to assess the host range, prevalence and distribution of filarioid infections in wild carnivores present in Romania. Between May 2014 and February 2016, 432 spleen samples originating from 14 species of wild carnivores have been tested for the presence of DNA of three species of filarioids (D. immitis, D. repens and A. reconditum). Overall 14 samples (3.24%) were molecularly positive. The most prevalent species was D. immitis (1.62%), accounting for 50% (n = 7) of the positive animals. The prevalence of D. repens was 1.39%, while that of A. reconditum was 0.23%. No co-infections were detected. Dirofilaria immitis DNA was detected in five golden jackals, Canis aureus (7.58%), one red fox, Vulpes vulpes (0.33%), and one wildcat, Felis silvestris (10%). The presence of D. repens DNA was detected in two red foxes (0.66%), two golden jackals (3.03%), one grey wolf (7.14%), and one least weasel, Mustela nivalis (33.33%). Acanthocheilonema reconditum DNA was found only in one red fox (0.33%). The present study provides molecular evidence of filarial infections in wild carnivore species in Romania, suggesting their potential epidemiological role and reports a new host species for D. repens.

  18. Evidence for natural recombination between mink enteritis virus and canine parvovirus

    Directory of Open Access Journals (Sweden)

    Wang Jianke

    2012-10-01

    Full Text Available Abstract A virus was isolated from mink showing clinical and pathological signs of enteritis in China. This virus, designated MEV/LN-10, was identified as mink enteritis virus (MEV based on its cytopathic effect in the feline F81 cell line, the hemagglutination (HA and hemagglutination inhibition (HI assay, electron microscopy (EM and animal infection experiments. The complete viral genome was cloned and sequenced. Phylogenetic and recombination analyses on the complete MEV/LN-10 genome showed evidence of recombination between MEV and canine parvovirus (CPV. The genome was composed of the NS1 gene originating from CPV while the VP1 gene was of MEV origin. This is the first demonstration of recombination between a CPV and MEV in nature. Our findings not only provide valuable evidence indicating that recombination is an important genetic mechanism contributing to the variation and evolution of MEV, but also that heterogeneous recombination can occur in the feline parvovirus subspecies.

  19. Using energy budgets to combine ecology and toxicology in a mammalian sentinel species

    Science.gov (United States)

    Desforges, Jean-Pierre W.; Sonne, Christian; Dietz, Rune

    2017-04-01

    Process-driven modelling approaches can resolve many of the shortcomings of traditional descriptive and non-mechanistic toxicology. We developed a simple dynamic energy budget (DEB) model for the mink (Mustela vison), a sentinel species in mammalian toxicology, which coupled animal physiology, ecology and toxicology, in order to mechanistically investigate the accumulation and adverse effects of lifelong dietary exposure to persistent environmental toxicants, most notably polychlorinated biphenyls (PCBs). Our novel mammalian DEB model accurately predicted, based on energy allocations to the interconnected metabolic processes of growth, development, maintenance and reproduction, lifelong patterns in mink growth, reproductive performance and dietary accumulation of PCBs as reported in the literature. Our model results were consistent with empirical data from captive and free-ranging studies in mink and other wildlife and suggest that PCB exposure can have significant population-level impacts resulting from targeted effects on fetal toxicity, kit mortality and growth and development. Our approach provides a simple and cross-species framework to explore the mechanistic interactions of physiological processes and ecotoxicology, thus allowing for a deeper understanding and interpretation of stressor-induced adverse effects at all levels of biological organization.

  20. Outbreaks of influenza A virus in farmed mink (Neovison vison) in Denmark: molecular characterization of the viruses

    DEFF Research Database (Denmark)

    Larsen, Lars Erik; Breum, Solvej Østergaard; Trebbien, Ramona

    2012-01-01

    that the virus was a human/swine reassortant, with the H and N gene most related to human H3N2 viruses circulating in 2005. The remaining 6 genes were most closely related to H1N2 influenza viruses circulating in Danish swine. This virus had not previously been described in swine, mink or humans. PCRs assays...... specifically targeting the new reassortant were developed and used to screen influenza positive samples from humans and swine in Denmark with negative results. Thus, there was no evidence that this virus had spread to humans or was circulating in Danish pigs. In 2010 and 2011, influenza virus was again...... diagnosed in diseased mink in a few farms. The genetic typing showed that the virus was similar to the pandemic H1N1 virus circulating in humans and swine. The H3N2 virus was not detected in 2010 and 2011. Taken together, these findings indicate that mink is highly susceptible for influenza A virus of human...

  1. Pathogenesis of canine distemper virus in experimentally infected raccoon dogs, foxes, and minks.

    Science.gov (United States)

    Zhao, Jianjun; Shi, Ning; Sun, Yangang; Martella, Vito; Nikolin, Veljko; Zhu, Chunsheng; Zhang, Hailing; Hu, Bo; Bai, Xue; Yan, Xijun

    2015-10-01

    Canine distemper virus (CDV) infects a broad range of carnivores and causes a highly contagious disease with severe immunosuppression. The disease severity markedly varies in different species. To investigate the pathogenesis of CDV in raccoon dog (Nyctereutes procyonoides), fox (Vulpes vulpes) and mink (Neovison vison) species, three groups of CDV sero-negative animals were infected with CDV strain LN(10)1. This CDV strain belongs to the Asia-1 genotype, which is epidemiologically predominant in carnivores in China. CDV infection provoked marked differences in virulence in the three species that were studied. Raccoon dogs developed fever, severe conjunctivitis, and pathological lesions, with 100% (5/5) mortality and with high viral RNA loads in organs within 15 days post infection (dpi). In infected foxes, the onset of the disease was delayed, with 40% (2/5) mortality by 21 dpi. Infected minks developed only mild clinical signs and pathological lesions, and mortality was not observed. Raccoon dogs and foxes showed more severe immune suppression (lymphopenia, decreased lymphocyte proliferation, viremia and low-level virus neutralizing antibodies) than minks. We also observed a distinct pattern of cytokine mRNA transcripts at different times after infection. Decreased IFN-γ and IL-4 mRNA responses were evident in the animals with fatal disease, while up-regulation of these cytokines was observed in the animals surviving the infection. Increased TNF-α response was detected in animals with mild or severe clinical signs. Based on the results, we could distinguish three different patterns of disease after experimental CDV infection, e.g. a mild form in minks, a moderate form in foxes and a severe disease in raccoon dogs. The observed differences in susceptibility to CDV could be related to distinct host cytokine profiles. Comparative evaluation of CDV pathogenesis in various animal species is pivotal to generate models suitable for the evaluation of CDV

  2. Comparison of American mink embryonic stem and induced pluripotent stem cell transcriptomes

    DEFF Research Database (Denmark)

    Menzorov, Aleksei G; Matveeva, Natalia M.; Markakis, Marios Nektarios

    2015-01-01

    BACKGROUND: Recently fibroblasts of many mammalian species have been reprogrammed to pluripotent state using overexpression of several transcription factors. This technology allows production of induced pluripotent stem (iPS) cells with properties similar to embryonic stem (ES) cells....... The completeness of reprogramming process is well studied in such species as mouse and human but there is not enough data on other species. We produced American mink (Neovison vison) ES and iPS cells and compared these cells using transcriptome analysis. RESULTS: We report the generation of 10 mink ES and 22 i......PS cell lines. The majority of the analyzed cell lines had normal diploid chromosome number. The only ES cell line with XX chromosome set had both X-chromosomes in active state that is characteristic of pluripotent cells. The pluripotency of ES and iPS cell lines was confirmed by formation of teratomas...

  3. Studies on the pathogenesis of Aleutian disease of mink. X. demonstration of immune complexes by the /sup 125/I-C 1 q binding test after experimental infection

    Energy Technology Data Exchange (ETDEWEB)

    Mueller-Peddinghaus, R [Kali-Chemie Pharma G.m.b.H., Hannover (Germany, F.R.). Abt. fuer Experimentelle Pathologie; Meyer zu Schwabedissen, H [Medizinische Hochschule Hannover (Germany, F.R.). Abt. fuer Klinische Immunologie und Bluttransfusionswesen; Kalden, J R [Erlangen-Nuernberg Univ., Erlangen (Germany, F.R.). Inst. und Poliklinik fuer Klinische Immunologie; Trautwein, G; Ueberschaer, S [Tieraerztliche Hochschule Hannover (Germany, F.R.). Inst. fuer Pathologie

    1980-01-01

    Aleutian disease (AD) of mink most closely resembles systemic lupus erythematosus (SLE) in man; both are immune complex disease. In experimental AD serum immune complexes are determined by the /sup 125/J-C 1 q-binding test using human C 1 q. Mink (n = 12) infected intraperitoneally with Aleutian disease virus (ADV), grown in fetal mink kidney cells, developed during the course of infection a mean of /sup 125/I-C 1 q serum binding equivalent to 3.62 +- 1.68 mg./ml. aggr. HGG. (aggregated human immunoglobulin). Sera of mink (n = 8) which were infected with ADV grown in L-cells showed a less marked /sup 125/I-C 1 q binding with a mean equivalent to 2.52 +- 1.43 mg./ml. aggr. HGG. In contrast control animals (n = 8) treated with non-ADV-infected mink epidermal fibroblasts or Eagle's minimal essential medium substituted with fetal calf serum only bound /sup 125/I-C 1 q equivalent to 1.02 +- 0.99 mg./ml. aggr. HGG. In mink infected with ADV propagated in fetal mink kidney cells a constant increase in the /sup 125/I-C 1 q serum binding occurred from the 4th to the 7th and 13th week after ADV infection. Mink which were infected with ADV propagated in mouse L-cells exhibited a different pattern of the /sup 125/I-C 1 q serum binding capacity with a sharp increase from the 4th to the 7th week, followed by a decline towards the 13th week post infection. The serum /sup 125/I-C 1 q binding capacity of all experimental animal groups exhibited at different times of the experiment a significant correlation with the presence of hypergammaglobulinaemia and raised ADV-antibody titers. From the data obtained it appears that the /sup 125/I-C 1 q binding test, utilizing human C 1 q, is a suitable method for the detection of circulating serum immune complexes in mink during the course of ADV-infection.

  4. Mink S38G Gene Polymorphism and Atrial Fibrillation in the Chinese Population: A Meta-Analysis of 1871 Participants

    Directory of Open Access Journals (Sweden)

    Yan-yan Li

    2014-01-01

    Full Text Available Mink gene S38G polymorphism in the β-subunit of slow activating component of the delayed rectifier potassium channel current potassium channel has been associated with increased atrial fibrillation (AF risk. However, the individual studies results were still controversial. To investigate the association of Mink S38G gene polymorphisms with AF, a meta-analysis including 1871 subjects from six individual studies was conducted. Mink S38G gene polymorphism was significantly related to AF under allelic (OR: 1.380, 95% CI: 1.200–1.600, P<0.00001, recessive (OR: 1.193, 95% CI: 1.033–1.377, P=0.017, dominant (OR: 1.057, 95% CI: 1.025–1.089, P<0.00001, additive (OR: 1.105, 95% CI: 1.036–1.178, P=0.002, homozygous (OR: 1.128, 95% CI: 1.068–1.191, P<0.00001, and heterozygous genetic models (OR: 1.078, 95% CI: 1.014–1.146, P=0.016. A significant association between Mink S38G gene polymorphism and AF risk was found. G allele carriers may predispose to AF.

  5. Genomic selection in mink yield higher accuracies with a Bayesian approach allowing for heterogeneous variance than a GBLUP model

    DEFF Research Database (Denmark)

    Villumsen, Trine Michelle; Su, Guosheng; Cai, Zexi

    2018-01-01

    by sequencing. Four live grading traits and four traits on dried pelts for size and quality were analysed. GWAS analysis detected significant SNPs for all the traits. The single-trait Bayesian model resulted in higher accuracies for the genomic predictions than the single-trait GBLUP model, especially......The accuracy of genomic prediction for mink was compared for single-trait and multiple-trait GBLUP models and Bayesian models that allowed for heterogeneous (co)variance structure over the genome. The mink population consisted of 2,103 brown minks genotyped with the method of genotyping...... for the traits measured on dried pelts. We expected the multiple-trait models to be superior to the single trait models since the multiple-trait model can make use of information when traits are correlated. However, we did not find a general improvement in accuracies with the multiple-trait models compared...

  6. S-phase-dependent cell cycle disturbances caused by Aleutian mink disease parvovirus

    DEFF Research Database (Denmark)

    Oleksiewicz, M.B.; Alexandersen, Søren

    1997-01-01

    We examined replication of the autonomous parovirus Aleutian mink disease parovirus (ADV) in relation to cell cycle progression of permissive Crandell feline kidney (CRFK) cells. Flow cytometric analysis showed that ADV caused a composite, binary pattern of cell cycle arrest. ADV-induced cell cyc...

  7. Outbreaks of Influenza A Virus in Farmed Mink (Neovison vison) in Denmark: Molecular characterization of the involved viruses

    DEFF Research Database (Denmark)

    Larsen, Lars Erik; Breum, Solvej Østergaard; Trebbien, Ramona

    mink farms with respiratory symptoms. Full-genome sequencing showed that the virus was a human/swine reassortant, with the H and N gene most related to human H3N2 viruses circulating in 2005. The remaining 6 genes were most closely related to H1N2 influenza viruses circulating in Danish swine....... This virus had not previously been described in swine, mink nor humans. PCRs assays specifically targeting the new reassortant were developed and used to screen influenza positive samples from humans and swine in Denmark with negative results. Thus, there was no evidence that this virus had spread to humans...... or was circulating in Danish pigs. In 2010 and 2011, influenza virus was again diagnosed in diseased mink in a few farms. The genetic typing showed that the virus was similar to the pandemic H1N1 virus circulating in humans and swine. The H3N2 virus was not detected in 2010 and 2011. Taken together, these findings...

  8. Electrochemotherapy with bleomycin of different types of cutaneous tumours in a ferret (Mustela putorius furo

    Directory of Open Access Journals (Sweden)

    Racnik Jozko

    2017-12-01

    Full Text Available Mast cell tumour, sebaceous gland adenoma, and less common squamous papilloma are skin tumours in ferrets (Mustela putorius furo, and early excisional surgery is usually the treatment of choice. The aim of our study was to investigate the effectiveness of electrochemotherapy (ECT, a new, minimally invasive non-surgical method, as first treatment option of different types of ferret skin tumours located on surgically difficult sites.

  9. Immunization with plasmid DNA encoding the hemagglutinin and the nucleoprotein confers robust protection against a lethal canine distemper virus challenge.

    Science.gov (United States)

    Dahl, Lotte; Jensen, Trine Hammer; Gottschalck, Elisabeth; Karlskov-Mortensen, Peter; Jensen, Tove Dannemann; Nielsen, Line; Andersen, Mads Klindt; Buckland, Robin; Wild, T Fabian; Blixenkrone-Møller, Merete

    2004-09-09

    We have investigated the protective effect of immunization of a highly susceptible natural host of canine distemper virus (CDV) with DNA plasmids encoding the viral nucleoprotein (N) and hemagglutinin (H). The combined intradermal and intramuscular routes of immunization elicited high virus-neutralizing serum antibody titres in mink (Mustela vison). To mimic natural exposure, we also conducted challenge infection by horizontal transmission from infected contact animals. Other groups received a lethal challenge infection by administration to the mucosae of the respiratory tract and into the muscle. One of the mink vaccinated with N plasmid alone developed severe disease after challenge. In contrast, vaccination with the H plasmid together with the N plasmid conferred solid protection against disease and we were unable to detect CDV infection in PBMCs or in different tissues after challenge. Our findings show that DNA immunization by the combined intradermal and intramuscular routes can confer solid protective immunity against naturally transmitted morbillivirus infection and disease.

  10. Abortion and mortality in farm mink (Neovison vison) associated with feed-born Clostridium limosum

    DEFF Research Database (Denmark)

    Hammer, Anne Sofie; Andresen, Lars; Aalbaek, Bent

    2017-01-01

    Disease in mink clinically characterized by abortion and increased mortality among pregnant female mink on 28 Danish farms was observed during April and May 2015. Most of these farms suffered extensive disease problems, including a significant increase in the number of mated females without litters....... Pathological, microbiological and molecular biological methods were applied to investigate the cause of disease. Necropsies of animals found dead revealed fragile and partially dissolved (liquefying) uterine tissue, with the presence of Gram positive rod-shaped bacteria. These slow growing bacteria were...... isolated by anaerobic culturing and identified as Clostridium limosum by both MALDI-TOF mass spectrometry analysis and 16S rRNA gene sequencing. All the performed tests for relevant differential diagnoses were negative. Foodborne disease was indicated because all the affected farms were served by the same...

  11. Farmers’ perception of stable schools as a tool to improve management for the benefit of mink welfare

    DEFF Research Database (Denmark)

    Henriksen, Britt I. F.; Anneberg, Inger; Sørensen, Jan Tind

    2015-01-01

    The aim of the study was to explore farmers' perception of stable schools as a tool to improve management for the benefit of mink welfare. Stable schools are knowledge exchange between farmers working towards a common goal, being able to give practical advice to each other. The concept is based......, and that motivation for working towards a common goal is very important for the process of common learning among the farmers. The uniform production system at mink farms gives special challenges in how to work with the different subjects to ensure farmer ownership of the process. The farmers did not see the seasonal...

  12. Accumulation of dietary fish fatty acids in the body fat reserves of some carnivorous fur-bearing animals

    Directory of Open Access Journals (Sweden)

    Kirsti Rouvinen

    1992-09-01

    Full Text Available Body fat composition of the mink (Mustela vison, polecat (Mustela putorius, and the raccoon dog (Nyctereutes procyonoides was studied. The animals were fed a wet diet, supplemented with 5 % lard (LA or fish oil (FO for 5-6 months. At pelting, five animals per dietary group were sampled. Dietary levels of cetoleic (C22:1ω11, eicosapentaenoic (EPA, C20:5ω3, and docosahexaenoic (DHA, C22:6ω3 acids were 0.4, 0.3, and 0.5% in the fat of the LA diet, and 7.6, 4.2 and 4.3% in the FO diet, respectively. In the FO diet, EPA and DHA accumulated especially in the liver and heart, while cetoleic acid showed the highest affinity to the heart muscle and subcutaneous fat. The highest levels of EPA were found in raccoon dogs and polecats fed the FO diet. The mean EPA levels ranged from 6.7-9.3% in the liver fat and 7.2-8.0% in the heart muscle fat. In the mink, the corresponding values were 2.7% and 3.9%, respectively. DHA levels were the highest in the liver fat of the polecats, being 18.5% in the FO diet. In addition, the liver in raccoon dogs fed the FO diet (13.8% differed significantly from themink (9.4%. The differences in the accumulation of these long-chained marine fatty acids were apparently caused by species differences in the efficiency of their peroxisomal β-oxidation.

  13. Expression of Aleutian mink disease parvovirus capsid proteins in defined segments: localization of immunoreactive sites and neutralizing epitopes to specific regions.

    Science.gov (United States)

    Bloom, M E; Martin, D A; Oie, K L; Huhtanen, M E; Costello, F; Wolfinbarger, J B; Hayes, S F; Agbandje-McKenna, M

    1997-01-01

    The capsid proteins of the ADV-G isolate of Aleutian mink disease parvovirus (ADV) were expressed in 10 nonoverlapping segments as fusions with maltose-binding protein in pMAL-C2 (pVP1, pVP2a through pVP2i). The constructs were designed to capture the VP1 unique sequence and the portions analogous to the four variable surface loops of canine parvovirus (CPV) in individual fragments (pVP2b, pVP2d, pVP2e, and pVP2g, respectively). The panel of fusion proteins was immunoblotted with sera from mink infected with ADV. Seropositive mink infected with either ADV-TR, ADV-Utah, or ADV-Pullman reacted preferentially against certain segments, regardless of mink genotype or virus inoculum. The most consistently immunoreactive regions were pVP2g, pVP2e, and pVP2f, the segments that encompassed the analogs of CPV surface loops 3 and 4. The VP1 unique region was also consistently immunoreactive. These findings indicated that infected mink recognize linear epitopes that localized to certain regions of the capsid protein sequence. The segment containing the hypervariable region (pVP2d), corresponding to CPV loop 2, was also expressed from ADV-Utah. An anti-ADV-G monoclonal antibody and a rabbit anti-ADV-G capsid antibody reacted exclusively with the ADV-G pVP2d segment but not with the corresponding segment from ADV-Utah. Mink infected with ADV-TR or ADV-Utah also preferentially reacted with the pVP2d sequence characteristic of that virus. These results suggested that the loop 2 region may contain a type-specific linear epitope and that the epitope may also be specifically recognized by infected mink. Heterologous antisera were prepared against the VP1 unique region and the four segments capturing the variable surface loops of CPV. The antisera against the proteins containing loop 3 or loop 4, as well as the anticapsid antibody, neutralized ADV-G infectivity in vitro and bound to capsids in immune electron microscopy. These results suggested that regions of the ADV capsid proteins

  14. Metabolic and growth response of mink (Neovison vison) kits until 10 weeks of age when exposed to different dietary protein provision

    DEFF Research Database (Denmark)

    Larsson, Caroline; Fink, Rikke; Matthiesen, Connie Marianne Frank

    2012-01-01

    to solid feed. The capacity to regulate the rate of gluconeogenesis was even more limited in young mink kits than in adult dams. However, young mink kits can regulate protein oxidation in response to dietary protein supply, probably by adapting the size of the liver and kidneys to the level of protein......Growth performance and metabolism were investigated in mink kits (n = 210) exposed to the same dietary treatment as their dams (n = 30), i.e. high (HP; 61% of metabolisable energy, ME), medium (MP; 48% of ME) or low (LP; 30% of ME) protein supply, from birth until 10 weeks of age. The kits were...... the heaviest. After transition to solid feed MP kits weighed most at nine weeks of age (p age, the kits fed the LP diet retained less (p

  15. Full protection in mink against mink enteritis virus with new generation canine parvovirus vaccines based on synthetic peptide or recombinant protein

    DEFF Research Database (Denmark)

    Langeveld, J. P.; Kamstrup, Søren; Uttenthal, Åse

    1995-01-01

    Two recently developed vaccines—one based on synthetic peptide and one based on recombinant capsid protein—fully protected dogs against heavy experimental canine parvovirus (CPV) infection. The high sequence homology (>98%) and antigenic similarity between CPV and mink enteritis virus (MEV), feline...... on inactivated virus. Surprisingly, this protection was obtained after only a single injection. Furthermore, the vaccinal dose of 150 μg of conjugated peptide or 3 μg of recombinant VP2 particles per animal, are sufficiently low to be cost-effective and applicable on a large scale....

  16. Investigation of the presence of human or bovine respiratory syncytial virus in the lungs of mink (Neovison vison) with hemorrhagic pneumonia due to Pseudomonas aeruginosa

    DEFF Research Database (Denmark)

    Salomonsen, Charlotte Mark; Breum, Solvej Østergaard; Larsen, Lars Erik

    2012-01-01

    Background Hemorrhagic pneumonia is a disease of farmed mink (Neovison vison) caused by Pseudomonas aeruginosa. The disease is highly seasonal in Danish mink with outbreaks occurring almost exclusively in the autumn. Human respiratory syncytial virus (RSV) has been shown to augment infection with...

  17. Hybridization and the phylogenetic relationship between polecats and domestic ferrets in Britain

    Science.gov (United States)

    Davison, A.; Birks, J. D. S.; Griffiths, H. I.; Kitchener, A. C.; Biggins, D.; Butlin, R. K.

    1999-01-01

    Ferrets (Mustela furo) were domesticated from polecats (M. putorius, M. eversmannii) over 2000 years ago. Following their introduction to Britain, they escaped and hybridized with native European polecats (M. putorius). Native polecats declined to the point of near extinction prior to World War I, but have recently begun to expand from a Welsh refugium. Concern has arisen as to the extent of polecat/ferret introgression, and in particular, whether the expanding population is of mainly hybrid origin. Therefore, mitochondrial DNA sequencing was used to investigate polecat genetic diversity in Britain. Two geographically distinct lineages were found, where one may be ancestral to the British polecat, and the other to the domestic ferret. The ancestral distribution of each lineage, or assortative mating is sufficient to explain the observed pattern. A further comparison between the distribution of the polecat phenotype and mitochondrial haplotype implies that the current population expansion may be mediated by dispersing male polecats hybridizing with female feral ferrets. However, the wild source of the ferret remains obscure. Relatively recent speciation from European mink (M. lutreola) and black-footed ferrets (M. nigripes), and/or the effects of hybridization result in an unresolved molecular phylogeny.

  18. The first record of Molineus patens (Dujardin, 1845) (Nematoda, Molineidae) in the ermine (Mustela erminea L.) in Poland.

    Science.gov (United States)

    Popiołek, Marcin; Jarnecki, Hubert; Łuczyński, Tomasz

    2009-01-01

    A single specimen of the nematode Molineus patens (Dujardin, 1845) was isolated from the intestine of the ermine (Mustela erminea L.) found dead on a road in Lubuskie voivodeship (Western Poland) in July 2008. Since this is the first record of the parasite in the ermine from Poland, description, biometrical data and figures are given.

  19. Effect of HMB and 2-Ox administered during pregnancy on bone properties in primiparous and multiparous minks (Neivison vison

    Directory of Open Access Journals (Sweden)

    Tomaszewska Ewa

    2015-12-01

    Full Text Available The aim of the study was to determine the mechanical and geometric properties as well as bone tissue density of long bones in primiparous and multiparous dams of minks supplemented with β-hydroxy β-methylbutyrate (HMB and/or 2-oxoketoglutarate (2-Ox during gestation. Powdered 2-Ox was given at the daily dosage of 0.4 g/kg b.w. separately or simultaneously with HMB, which was administered at the daily dosage of 0.02 g/kg b.w. The study demonstrates for the first time that administration of 2-Ox and/or HMB to dams markedly influences bone tissue density and the mechanical and geometrical properties of mother`s bones in minks. Moreover, it was demonstrated that the supplementation was more effective in the thoracic limb, which was comprehensively used in contrast to the pelvic limb. The mechanical parameters and bone tissue density significantly increased in the humerus in multiparous minks. Only such diet may provide satisfactory production results in the animals. Nutritional deficiencies occurring during pregnancies may trigger body`s own reserves to cover the bone mass increase in developing foetuses and support milk production. This can prevent regeneration of dams’ organisms, which negatively affects their reproductive performance. 2-Ox or HMB may be regarded as a protective metabolite when administered orally to minks, counteracting the negative influences of pregnancy and lactation periods on bones condition. Both simultaneous treatment with 2-Ox and HMB and their separate administration were equally effective.

  20. Canine distemper virus DNA vaccination of mink can overcome interference by maternal antibodies

    DEFF Research Database (Denmark)

    Jensen, Trine Hammer; Nielsen, Line; Aasted, Bent

    2015-01-01

    Canine distemper virus (CDV) is highly contagious and can cause severe disease against which conventional live vaccines are ineffective in the presence of maternal antibodies. Vaccination in the presences of maternal antibodies was challenged by vaccination of 5 days old and 3 weeks old mink kits...

  1. Development of a sandwich ELISA for quantification of immunoglobulin G in mink blood

    DEFF Research Database (Denmark)

    Mathiesen, Ronja; Chriél, Mariann; Struve, T.

    2016-01-01

    early immunity and thus their resistance against pathogenic agents found in the environment. This study describes a sandwich ELISA for quantification of the concentration of total immunoglobulin G in mink blood. The ELISA was validated with serum samples from females (n=8) and their kits (litters of 4...

  2. Reduced Genetic Diversity and Increased Structure in American Mink on the Swedish Coast following Invasive Species Control.

    Science.gov (United States)

    Zalewski, Andrzej; Zalewska, Hanna; Lunneryd, Sven-Gunnar; André, Carl; Mikusiński, Grzegorz

    2016-01-01

    Eradication and population reductions are often used to mitigate the negative impacts of non-native invasive species on native biodiversity. However, monitoring the effectiveness of non-native species control programmes is necessary to evaluate the efficacy of these measures. Genetic monitoring could provide valuable insights into temporal changes in demographic, ecological, and evolutionary processes in invasive populations being subject to control programmes. Such programmes should cause a decrease in effective population size and/or in genetic diversity of the targeted non-native species and an increase in population genetic structuring over time. We used microsatellite DNA data from American mink (Neovison vison) to determine whether the removal of this predator on the Koster Islands archipelago and the nearby Swedish mainland affected genetic variation over six consecutive years of mink culling by trappers as part of a population control programme. We found that on Koster Islands allelic richness decreased (from on average 4.53 to 3.55), genetic structuring increased, and effective population size did not change. In contrast, the mink population from the Swedish coast showed no changes in genetic diversity or structure, suggesting the stability of this population over 6 years of culling. Effective population size did not change over time but was higher on the coast than on the islands across all years. Migration rates from the islands to the coast were almost two times higher than from the coast to the islands. Most migrants leaving the coast were localised on the southern edge of the archipelago, as expected from the direction of the sea current between the two sites. Genetic monitoring provided valuable information on temporal changes in the population of American mink suggesting that this approach can be used to evaluate and improve control programmes of invasive vertebrates.

  3. Reduced Genetic Diversity and Increased Structure in American Mink on the Swedish Coast following Invasive Species Control.

    Directory of Open Access Journals (Sweden)

    Andrzej Zalewski

    Full Text Available Eradication and population reductions are often used to mitigate the negative impacts of non-native invasive species on native biodiversity. However, monitoring the effectiveness of non-native species control programmes is necessary to evaluate the efficacy of these measures. Genetic monitoring could provide valuable insights into temporal changes in demographic, ecological, and evolutionary processes in invasive populations being subject to control programmes. Such programmes should cause a decrease in effective population size and/or in genetic diversity of the targeted non-native species and an increase in population genetic structuring over time. We used microsatellite DNA data from American mink (Neovison vison to determine whether the removal of this predator on the Koster Islands archipelago and the nearby Swedish mainland affected genetic variation over six consecutive years of mink culling by trappers as part of a population control programme. We found that on Koster Islands allelic richness decreased (from on average 4.53 to 3.55, genetic structuring increased, and effective population size did not change. In contrast, the mink population from the Swedish coast showed no changes in genetic diversity or structure, suggesting the stability of this population over 6 years of culling. Effective population size did not change over time but was higher on the coast than on the islands across all years. Migration rates from the islands to the coast were almost two times higher than from the coast to the islands. Most migrants leaving the coast were localised on the southern edge of the archipelago, as expected from the direction of the sea current between the two sites. Genetic monitoring provided valuable information on temporal changes in the population of American mink suggesting that this approach can be used to evaluate and improve control programmes of invasive vertebrates.

  4. Detection of intracellular canine distemper virus antigen in mink inoculated with an attenuated or a virulent strain of canine distemper virus.

    Science.gov (United States)

    Blixenkrone-Møller, M

    1989-09-01

    Using an indirect immunofluorescence technique, the distribution of viral antigen in various tissues and blood mononuclear leukocytes was studied in wild mink, either vaccinated with an attenuated vaccine strain of canine distemper virus (CDV) or experimentally inoculated with the virulent Snyder-Hill strain of CDV. Viral antigen was detected in cells of the lymphoid system 6 to 12 days after vaccination. From 2 to 3 days after inoculation with the virulent strain, CDV antigen was demonstrated in cells of the lymphoid system and, during the incubation period, the antigen had spread to the epithelia and brain at days 6 and 12, respectively. In clinical cases of acute fatal canine distemper, the viral antigen was detected in a wide variety of tissues, including the cells of the lymphoid system, epithelial cells of skin, mucous membranes, lung, kidney, and cells of the CNS. The diagnostic importance of CDV antigen detection is discussed on the basis of these findings.

  5. Rhinitis and disseminated disease in a ferret (Mustela putorius furo) naturally infected with Sarcocystis neurona.

    Science.gov (United States)

    Britton, Ann P; Dubey, J P; Rosenthal, Benjamin M

    2010-04-19

    Naturally occurring Sarcocystis neurona infection in a ferret (Mustela putorius furo) with rhinitis and disseminated disease are described for the first time. The ferret exhibited severe rhinitis with intra-lesional S. neurona merozoites and schizonts. Diagnosis was confirmed immunohistochemically by staining with S. neurona-specific antibodies, and by phylogenetic analyses of conserved and variable portions of nuclear ribosomal DNA. On the basis of intense schizogony in the nasal mucosa, we propose the possibility of an olfactory nerve pathway route of infection for S. neurona meningoencephalitis.

  6. Genetic and non-genetic indirect effects for bite mark traits in group housed mink

    DEFF Research Database (Denmark)

    Alemu, Setegn Worku; Berg, Peer; Janss, Luc

    irrespective of relatedness. Kin selection theory predicts that an individual will interact differently with family members vs. non-family members. We showed that mink interact differently either due to sex or the family relationship with their group mates. Our results show that IGEs are very important...

  7. The effects of climbing cages on behaviour of female mink during the lactation period

    DEFF Research Database (Denmark)

    Lidfors, L.; Axelsson, H.; Loberg, J.

    2012-01-01

    =46) or standard cages (2.550 cm², n=44). Behavioural observations were made four weeks before birth until kits were nine weeks old with one-zero sampling during two hours before feeding. During week 1-4 after females had given birth they were mostly recorded in the nest box and there were......The aim was to investigate if there were differences in behaviour of female mink when kept in a climbing cage compared with a standard cage during the lactation period. The study was carried out on 90 mink of the colour type "black cross". Females were housed in either climbing cages (4.350 cm², n...... no differences between cage types. Week 5-8 after giving birth females in climbing cages were less in the nest box, less active out in the cage and had fewer abnormal behaviours, but were on the platforms and more inactive out in the cage. In the bottom cage females were more often walking, grooming and inactive...

  8. Diagnostic SNPs for inferring population structure in American mink (Neovison vison) identified through RAD sequencing

    DEFF Research Database (Denmark)

    2015-01-01

    Data from: "Diagnostic SNPs for inferring population structure in American mink (Neovison vison) identified through RAD sequencing" in Genomic Resources Notes accepted 1 October 2014 to 30 November 2014....

  9. Cryptosporidium spp. em furão (Mustela putorius furo) no sul do Brasil

    OpenAIRE

    Fanfa, Vinicius da Rosa; Farret, Matheus Hillard; Silva, Aleksandro Schafer da; Monteiro, Silvia Gonzalez

    2010-01-01

    http://dx.doi.org/10.5007/2175-7925.2010v23n1p225 Este trabalho visou avaliar o parasitismo gastrintestinal em furão (Mustela putorius furo) mantido em cativeiro no sul do Brasil. Foram analisadas fezes de dois furões, macho e fêmea, com três anos de idade, através das técnicas de exame direto, centrífugo-flutuação com sulfato de zinco e a coloração pelo método de Kinyoun para pesquisa de parasitos. Nas amostras constatou-se a presença de oocistos de Cryptosporidium spp. Este caso refere-...

  10. Enzootic plague reduces black-footed ferret (Mustela nigripes) survival in Montana

    Science.gov (United States)

    Matchett, Marc R.; Biggins, Dean E.; Carlson, Valerie; Powell, Bradford; Rocke, Tonie E.

    2010-01-01

    Black-footed ferrets (Mustela nigripes) require extensive prairie dog colonies (Cynomys spp.) to provide habitat and prey. Epizootic plague kills both prairie dogs and ferrets and is a major factor limiting recovery of the highly endangered ferret. In addition to epizootics, we hypothesized that enzootic plague, that is, presence of disease-causing Yersinia pestis without any noticeable prairie dog die off, may also affect ferret survival. We reduced risk of plague on portions of two ferret reintroduction areas by conducting flea control for 3 years. Beginning in 2004, about half of the ferrets residing on dusted and nondusted colonies were vaccinated against plague with an experimental vaccine (F1-V fusion protein). We evaluated 6-month reencounter rates (percentage of animals observed at the end of an interval that were known alive at the beginning of the interval), an index to survival, for ferrets in four treatment groups involving all combinations of vaccination and flea control. For captive-reared ferrets (115 individuals observed across 156 time intervals), reencounter rates were higher for vaccinates (0.44) than for nonvaccinates (0.23, p = 0.044) on colonies without flea control, but vaccination had no detectable effect on colonies with flea control (vaccinates = 0.41, nonvaccinates = 0.42, p = 0.754). Flea control resulted in higher reencounter rates for nonvaccinates (p = 0.026), but not for vaccinates (p = 0.508). The enhancement of survival due to vaccination or flea control supports the hypothesis that enzootic plague reduces ferret survival, even when there was no noticeable decline in prairie dog abundance. The collective effects of vaccination and flea control compel a conclusion that fleas are required for maintenance, and probably transmission, of plague at enzootic levels. Other studies have demonstrated similar effects of flea control on several species of prairie dogs and, when combined with this study, suggest

  11. Host response during acute canine distemper virus infections in naive and DNA immunized mink

    DEFF Research Database (Denmark)

    Nielsen, Line

    SAMMENDRAG (DANISH SUMMARY) Mink blev inokuleret med to forskellige vildtype hundesygevirus (CDV) stammer fra forskellige genotyper. DK91 stammen repræsenter de europæiske genotyper isoleret efter 1990, mens Snyder Hill stammen repræsenter de gamle amerikanske genotyper isoleret før 1960. De to C...

  12. Pyometra in a Siberian Polecat (Mustela eversmanni)

    Science.gov (United States)

    Johnson, J.D.; Biggins, D.E.; Wrigley, R.H.; Mangone, B.A.; Wimsatt, J.

    1999-01-01

    A 2-year-old Siberian polecat (Mustela eversmanni) from a breeding colony presented for ultrasound evaluation for pregnancy. It was paired with a male for 2.75 months and had remained absent of pregnancy signs when it was anesthetized and clinically evaluated. Until this time, the animal had eaten well and shown no outward signs of debility. On palpation, the animal had a fluid-filled tubular structure in the caudal abdomen, consistent in location and size with the uterus. No sign of vaginal discharge was present. Ultrasonography revealed 10 fluid-filled evaginations (approximately 12 mm in diameter) of the uterine horns. A presumptive diagnosis of a fluid-filled reproductive tract and likely reproductive failure was made in light of the animal's history, its clinical signs, and the ultrasound findings. Euthanasia was performed because the animal was nonreproductive and might yield information relevant to the breeding colony as a whole. Necropsy of the polecat revealed a distended fluctuant uterus containing mildly odiferous, thick, yellow-green, purulent material. Histopathology confirmed the diagnosis of pyometra. A pure and heavy growth of Enterococcus fecalis was cultured from the uterine contents. In light of results from routine minimal inhibitory concentration antibiotic sensitivity screening, this isolate was resistant to all antibiotics tested in the standard teaching hospital screen.

  13. Pyometra in a Siberian Polecat (Mustela eversmanni).

    Science.gov (United States)

    Johnson, Jay D.; Biggins, Dean E.; Wrigley, Robert H.; Mangone, Berney A.; Wimsatt, Jeffrey

    1999-11-01

    A 2-year-old Siberian polecat (Mustela eversmanni) from a breeding colony presented for ultrasound evaluation for pregnancy. It was paired with a male for 2.75 months and had remained absent of pregnancy signs when it was anesthetized and clinically evaluated. Until this time, the animal had eaten well and shown no outward signs of debility. On palpation, the animal had a fluid-filled tubular structure in the caudal abdomen, consistent in location and size with the uterus. No sign of vaginal discharge was present. Ultrasonography revealed 10 fluid-filled evaginations (approximately 12 mm in diameter) of the uterine horns. A presumptive diagnosis of a fluid-filled reproductive tract and likely reproductive failure was made in light of the animal's history, its clinical signs, and the ultrasound findings. Euthanasia was performed because the animal was nonreproductive and might yield information relevant to the breeding colony as a whole. Necropsy of the polecat revealed a distended fluctuant uterus containing mildly odiferous, thick, yellow-green, purulent material. Histopathology confirmed the diagnosis of pyometra. A pure and heavy growth of Enterococcus fecalis was cultured from the uterine contents. In light of results from routine minimal inhibitory concentration antibiotic sensitivity screening, this isolate was resistant to all antibiotics tested in the standard teaching hospital screen.

  14. Advances in reproductive science for wild carnivore conservation.

    Science.gov (United States)

    Comizzoli, P; Crosier, A E; Songsasen, N; Gunther, M Szykman; Howard, J G; Wildt, D E

    2009-07-01

    Knowledge about reproduction is critical for predicting the viability of wildlife populations in nature and for managing breeding programmes in captivity. Intensive species-based studies are the priority, because reproductive mechanisms are extraordinarily diverse, even within the same taxonomic family. Carnivores deserve more attention as such species are highly vulnerable to environmental change and human persecution. The present review provides contemporary illustrations of how reproductive science is contributing to understand unique reproductive mechanisms that are both of fundamental and applied interest. In the case of the endangered African wild dog (Lycaon pictus) free-living in South Africa, non-invasive faecal corticosteroid assessments have yielded new insights about the impact of animal relocation and reintroduction on adaptive responses, reproductive fitness and survival. For the maned wolf (Chrysocyon brachyurus), advances have been made in characterizing and comparing reproductive traits in free-ranging vs captive individuals. For the cheetah (Acinonyx jubatus), recent studies have focused on the cryosensitivity of sperm and the ability to develop a field-friendly sperm cryo-method. The by-product has been a large-scale frozen repository of sperm from wild-caught cheetahs useful for infusing new genes into ex situ populations. Finally, rigorous, multi-disciplinary and cross-institutional reproductive studies of the black-footed ferret (Mustela nigripes), including the use of artificial insemination, have contributed to the remarkable recovery and restoration of this species, once on the brink of extinction. In summary, advances in reproductive science are not necessarily related to 'assisted breeding'. However, understanding the unique ways of carnivore reproduction greatly contributes to species management and conservation.

  15. Effects of dietary protein level on growth, health and physiological parameters in growing-furring mink

    DEFF Research Database (Denmark)

    Damgaard, Birthe Marie; Larsen, Peter F.; Clausen, Tove

    2012-01-01

    The aim of the study was to investigate the effects of the dietary protein level and the feeding strategy on growth, health and physiological blood and liver parameters in growing-furring male mink. Effects of dietary protein levels ranging from 22% of metabolizable energy (MEp) to experimental p...

  16. The welfare of farmed mink should be easy to assess in a correct way and lead to animal welfare improvements

    DEFF Research Database (Denmark)

    Henriksen, Britt

    2015-01-01

    A three year PhD-study in assessment of mink welfare has contributed with methods and knowledge on how to get the welfare assessments as efficient and correct as possible and how to use the assessments in order to increase the welfare of the animals. The study found, that it might be possible...... periods have the same effect of date of assessment. The study also found that mink farmers are generally positive towards the structural way of working in stable schools and that including a discussion of the WelFur results related to the different farms in a stable school will make the feedback...

  17. Estimates of the abundance of minke whales (Balaenoptera acutorostrata from Faroese and Icelandic NASS shipboard surveys

    Directory of Open Access Journals (Sweden)

    Daniel G Pike

    2009-09-01

    Full Text Available North Atlantic Sightings Surveys for cetaceans were carried out Northeast and Central Atlantic in 1987, 1989, 1995 and 2001. Here we provide estimates of density and abundance for minke whales from the Faroese and Icelandic ship surveys. The estimates are not corrected for availability or perception biases. Double platform data collected in 2001 indicates that perception bias is likely considerable for this species. However comparison of corrected estimates of densityfrom aerial surveys with a ship survey estimate from the same area suggests that ship surveys can be nearly unbiased under optimal survey conditions with high searching effort. There were some regional changes in density over the period but no overall changes in density and abundance. Given the recent catch history for minke whales in this area, we would not expect to see changes in abundance due to exploitation that would be detectable with these surveys.

  18. Species Identification of Fox-, Mink-, Dog-, and Rabbit-Derived Ingredients by Multiplex PCR and Real-Time PCR Assay.

    Science.gov (United States)

    Wu, Qingqing; Xiang, Shengnan; Wang, Wenjun; Zhao, Jinyan; Xia, Jinhua; Zhen, Yueran; Liu, Bang

    2018-05-01

    Various detection methods have been developed to date for identification of animal species. New techniques based on PCR approach have raised the hope of developing better identification methods, which can overcome the limitations of the existing methods. PCR-based methods used the mitochondrial DNA (mtDNA) as well as nuclear DNA sequences. In this study, by targeting nuclear DNA, multiplex PCR and real-time PCR methods were developed to assist with qualitative and quantitative analysis. The multiplex PCR was found to simultaneously and effectively distinguish four species (fox, dog, mink, and rabbit) ingredients by the different sizes of electrophoretic bands: 480, 317, 220, and 209 bp. Real-time fluorescent PCR's amplification profiles and standard curves showed good quantitative measurement responses and linearity, as indicated by good repeatability and coefficient of determination R 2  > 0.99. The quantitative results of quaternary DNA mixtures including mink, fox, dog, and rabbit DNA are in line with our expectations: R.D. (relative deviation) varied between 1.98 and 12.23% and R.S.D. (relative standard deviation) varied between 3.06 and 11.51%, both of which are well within the acceptance criterion of ≤ 25%. Combining the two methods is suitable for the rapid identification and accurate quantification of fox-, dog-, mink-, and rabbit-derived ingredients in the animal products.

  19. Construction of an American mink Bacterial Artificial Chromosome (BAC library and sequencing candidate genes important for the fur industry

    Directory of Open Access Journals (Sweden)

    Christensen Knud

    2011-07-01

    Full Text Available Abstract Background Bacterial artificial chromosome (BAC libraries continue to be invaluable tools for the genomic analysis of complex organisms. Complemented by the newly and fast growing deep sequencing technologies, they provide an excellent source of information in genomics projects. Results Here, we report the construction and characterization of the CHORI-231 BAC library constructed from a Danish-farmed, male American mink (Neovison vison. The library contains approximately 165,888 clones with an average insert size of 170 kb, representing approximately 10-fold coverage. High-density filters, each consisting of 18,432 clones spotted in duplicate, have been produced for hybridization screening and are publicly available. Overgo probes derived from expressed sequence tags (ESTs, representing 21 candidate genes for traits important for the mink industry, were used to screen the BAC library. These included candidate genes for coat coloring, hair growth and length, coarseness, and some receptors potentially involved in viral diseases in mink. The extensive screening yielded positive results for 19 of these genes. Thirty-five clones corresponding to 19 genes were sequenced using 454 Roche, and large contigs (184 kb in average were assembled. Knowing the complete sequences of these candidate genes will enable confirmation of the association with a phenotype and the finding of causative mutations for the targeted phenotypes. Additionally, 1577 BAC clones were end sequenced; 2505 BAC end sequences (80% of BACs were obtained. An excess of 2 Mb has been analyzed, thus giving a snapshot of the mink genome. Conclusions The availability of the CHORI-321 American mink BAC library will aid in identification of genes and genomic regions of interest. We have demonstrated how the library can be used to identify specific genes of interest, develop genetic markers, and for BAC end sequencing and deep sequencing of selected clones. To our knowledge, this is the

  20. Testing the WelFur assessment protocol for mink on-farm in three seasons of production

    DEFF Research Database (Denmark)

    Møller, Steen Henrik; Hansen, Steffen W

    2011-01-01

    principles and 12 underlying criteria, to be measured at the farm. Based on validity, reliability and feasibility 22 measures have been selected for use in the three seasons of mink production: 1. Breeders during winter, 2. Dams with kits during spring, and 3. Juveniles during growth in the autumn, in order...

  1. Ferro-manganese encrustation on the tympanic bulla of a minke whale from Central Indian Ocean Basin

    Digital Repository Service at National Institute of Oceanography (India)

    Banakar, V.K.

    Ear bone of a minke whale (Balaenoptera acutorostrata) was collected from 5200 m water depth with thin coating of Fe-Mn oxide on its surface. The Fe-Mn coating material was similar in nature and ratios Mn/Fe and Cu + Ni/Co were closely comparable...

  2. Estradiol stimulates glycogen synthesis whereas progesterone promotes glycogen catabolism in the uterus of the American mink (Neovison vison).

    Science.gov (United States)

    Bowman, Kole; Rose, Jack

    2017-01-01

    Glycogen synthesis by mink uterine glandular and luminal epithelia (GE and LE) is stimulated by estradiol (E 2 ) during estrus. Subsequently, the glycogen deposits are mobilized to near completion to meet the energy requirements of pre-embryonic development and implantation by as yet undetermined mechanisms. We hypothesized that progesterone (P 4 ) was responsible for catabolism of uterine glycogen reserves as one of its actions to ensure reproductive success. Mink were treated with E 2 , P 4 or vehicle (controls) for 3 days and uteri collected 24 h (E 2 , P 4 and vehicle) and 96 h (E 2 ) later. To evaluate E 2 priming, mink were treated with E 2 for 3 days, then P 4 for an additional 3 days (E 2 →P 4 ) and uteri collected 24 h later. Percent glycogen content of uterine epithelia was greater at E 2 + 96 h (GE = 5.71 ± 0.55; LE = 11.54 ± 2.32) than E 2 +24 h (GE = 3.63 ± 0.71; LE = 2.82 ± 1.03), and both were higher than controls (GE = 0.27 ± 0.15; LE = 0.54 ± 0.30; P glycogen content (GE = 0.61 ± 0.16; LE = 0.51 ± 0.13), to levels not different from controls, while concomitantly increasing catabolic enzyme (glycogen phosphorylase m and glucose-6-phosphatase) gene expression and amount of phospho-glycogen synthase protein (inactive) in uterine homogenates. Interestingly, E 2 →P 4 increased glycogen synthase 1 messenger RNA (mRNA) and hexokinase 1mRNA and protein. Our findings suggest to us that while E 2 promotes glycogen accumulation by the mink uterus during estrus and pregnancy, it is P 4 that induces uterine glycogen catabolism, releasing the glucose that is essential to support pre-embryonic survival and implantation. © 2016 Japanese Society of Animal Science.

  3. Radiographic evaluation of destructive periodontal disease in blue mink in relation to age and blood morphology

    DEFF Research Database (Denmark)

    Hammer, Anne Sofie; Andersen, Thomas Holmen; Eriksen, Thomas

    2005-01-01

    In this study, blood samples and jaws were collected from 2 genotypes of blue mink (n = 289) in order to examine phenotypic expression of specific characteristics of Chediak-Higashi Syndrome (C-HS). Blood samples were subjected to differential counts to assess the proportion of abnormal polymorph...

  4. Comparison of biological and genomic characteristics between a newly isolated mink enteritis parvovirus MEV-LHV and an attenuated strain MEV-L.

    Science.gov (United States)

    Mao, Yaping; Wang, Jigui; Hou, Qiang; Xi, Ji; Zhang, Xiaomei; Bian, Dawei; Yu, Yongle; Wang, Xi; Liu, Weiquan

    2016-06-01

    A virus isolated from mink showing clinical signs of enteritis was identified as a high virulent mink enteritis parvovirus (MEV) based on its biological characteristics in vivo and in vitro. Mink, challenged with this strain named MEV-LHV, exhibited severe pathological lesions as compared to those challenged with attenuated strain MEV-L. MEV-LHV also showed higher infection and replication efficiencies in vitro than MEV-L. Sequence of the complete genome of MEV-LHV was determined and analyzed in comparison with those in GenBank, which revealed that MEV-LHV shared high homology with virulent strain MEV SD12/01, whereas MEV-L was closely related to Abashiri and vaccine strain MEVB, and belonged to a different branch of the phylogenetic tree. The genomes of the two strains differed by insertions and deletions in their palindromic termini and specific unique mutations (especially VP2 300) in coding sequences which may be involved in viral replication and pathogenicity. The results of this study provide a better understanding of the biological and genomic characteristics of MEV and identify certain regions and sites that may be involved in viral replication and pathogenicity.

  5. Weaning and separation stress: maternal motivation decreases with litter age and litter size in farmed mink

    DEFF Research Database (Denmark)

    Malmkvist, Jens; Sørensen, Dennis Dam; Larsen, Torben

    2016-01-01

    was to investigate whether the dams experienced stress/had a different motivation to be reunited with the litter after7 and 8 weeks, estimated by non-invasive determination of cortisol (FCM: Faecal Cortisol Metabolites)and dam behaviour including calls the first week after separation (D0: Day of removal, D1: next......The optimal timing of separating the mink dam from the litter is suggested to be a balance between the partly conflicting needs of the mother and the kits. Early removal of the dam or partial removal of the litter may protect the dam against exhaustion. Little is, however, known about dam stress...... and maternal motivation around the time of weaning and separation. Therefore, we investigated effects of separating the dam from the litter using brown first-parity farm mink dams (n = 374) taken away from the litter either day 49 ± 1 (7w, n = 185) or day 56 ± 1 (8w, n = 189) after birth. The aim...

  6. Removal of American mink increases the success of simulated nests in linear habitat

    Czech Academy of Sciences Publication Activity Database

    Padyšáková, E.; Šálek, Martin; Poledník, L.; Sedláček, František; Albrecht, Tomáš

    2009-01-01

    Roč. 36, č. 3 (2009), s. 225-230 ISSN 1035-3712 R&D Projects: GA MŠk LC06073 Grant - others:GA MŽP(CZ) SE/620/1/03; GA MŽP(CZ) SP/2D3/60/08 Institutional research plan: CEZ:AV0Z60870520; CEZ:AV0Z60930519 Keywords : mink * nest predation * introduction * allien species Subject RIV: EH - Ecology, Behaviour Impact factor: 1.222, year: 2009

  7. Genetic diversity and phylogenetic analysis of Aleutian mink disease virus isolates in north-east China.

    Science.gov (United States)

    Leng, Xue; Liu, Dongxu; Li, Jianming; Shi, Kun; Zeng, Fanli; Zong, Ying; Liu, Yi; Sun, Zhibo; Zhang, Shanshan; Liu, Yadong; Du, Rui

    2018-05-01

    Aleutian mink disease is the most important disease in the mink-farming industry worldwide. So far, few large-scale molecular epidemiological studies of AMDV, based on the NS1 and VP2 genes, have been conducted in China. Here, eight new Chinese isolates of AMDV from three provinces in north-east China were analyzed to clarify the molecular epidemiology of AMDV. The seroprevalence of AMDV in north-east China was 41.8% according to counterimmuno-electrophoresis. Genetic variation analysis of the eight isolates showed significant non-synonymous substitutions in the NS1 and VP2 genes, especially in the NS1 gene. All eight isolates included the caspase-recognition sequence NS1:285 (DQTD↓S), but not the caspase recognition sequence NS1:227 (INTD↓S). The LN1 and LN2 strains had a new 10-amino-acid deletion in-between amino acids 28-37, while the JL3 strain had a one-amino-acid deletion at position 28 in the VP2 protein, compared with the AMDV-G strain. Phylogenetic analysis based on most of NS1 (1755 bp) and complete VP2 showed that the AMDV genotypes did not cluster according to their pathogenicity or geographic origin. Local and imported ADMV species are all prevalent in mink-farming populations in the north-east of China. This is the first study to report the molecular epidemiology of AMDV in north-east China based on most of NS1 and the complete VP2, and further provides information about polyG deletions and new variations in the amino acid sequences of NS1 and VP2 proteins. This report is a good foundation for further study of AMDV in China.

  8. A test of mink microsatellite markers in the ferret

    DEFF Research Database (Denmark)

    Anistoroaei, Razvan Marian; Christensen, Knud

    2006-01-01

    Short tandem repeats are a source of highly polymorphic markers in mammalian genomes. Genetic variations at these hypervariable loci is extensively used for linkage analysis and to identify individuals, and is very useful for interpopulation and interspecies studies. Fifty-nine microsatellite mar...... that were identical in size to those from mink displayed a high degree of conservation, with some differences at the repeat motif sites. These results could aid cross-utilization of markers between these two species.......Short tandem repeats are a source of highly polymorphic markers in mammalian genomes. Genetic variations at these hypervariable loci is extensively used for linkage analysis and to identify individuals, and is very useful for interpopulation and interspecies studies. Fifty-nine microsatellite...

  9. Early kit mortality and growth in farmed mink are affected by litter size rather than nest climate

    DEFF Research Database (Denmark)

    Schou, Toke Munk; Malmkvist, Jens

    2017-01-01

    increased growth (P=0.026). Nest box temperature had little effect on early kit survival and growth, which could be due to dams’ additional maternal behaviour. Therefore, we cannot confirm that temperature is the primary reason for kit mortality, under the conditions of plenty straw access for maternal nest......We investigated the effects of nest box climate on early mink kit mortality and growth. We hypothesised that litters in warm nest boxes experience less hypothermia-induced mortality and higher growth rates during the 1st week of life. This study included data from 749, 1-year-old breeding dams...... building. Instead, prenatal and/or parturient litter size is the primary factor influencing early kit vitality. The results indicate that the focus should be on litter size and dam welfare around the times of gestation and birth to increase early kit survival in farmed mink....

  10. Construction of an Americn mink Bacterial Artificial Chromosome (BAC) library and sequencing candidate genes important for the fur industry

    DEFF Research Database (Denmark)

    Anistoroaei, Razvan Marian; Hallers, Boudewijn ten; Nefedov, Michael

    2011-01-01

    BACKGROUND: Bacterial artificial chromosome (BAC) libraries continue to be invaluable tools for the genomic analysis of complex organisms. Complemented by the newly and fast growing deep sequencing technologies, they provide an excellent source of information in genomics projects. RESULTS: Here, we...... report the construction and characterization of the CHORI-231 BAC library constructed from a Danish-farmed, male American mink (Neovison vison). The library contains approximately 165,888 clones with an average insert size of 170 kb, representing approximately 10-fold coverage. High-density filters, each...... consisting of 18,432 clones spotted in duplicate, have been produced for hybridization screening and are publicly available. Overgo probes derived from expressed sequence tags (ESTs), representing 21 candidate genes for traits important for the mink industry, were used to screen the BAC library...

  11. Investigations into shaking mink syndrome: an encephalomyelitis of unknown cause in farmed mink (Mustela vison) kits in Scandinavia

    DEFF Research Database (Denmark)

    Gavier-Widen, Dolores; Brojer, Caroline; Dietz, Hans Henrik

    2004-01-01

    diseases (canine distemper, Borna disease, Louping ill, West Nile virus infection, tick-borne encephalitis, Aleutian disease), tests for protozoa (Toxoplasma gondii, Neospora caninum, Encephalitozoon cuniculi), bacteria (general culture, listeria, Clamydophila psittaci), and intracerebral inoculation...

  12. Subcellular localization of Aleutian mink disease parvovirus proteins and DNA during permissive infection of Crandell feline kidney cells

    DEFF Research Database (Denmark)

    Oleksiewicz, M.B.; Costello, F.; Huhtanen, M.

    1996-01-01

    Confocal microscopy allowed us to localize viral nonstructural (NS) and capsid (VP) proteins and DNA simultaneously in cells permissively infected with Aleutian mink disease parvovirus (ADV). Early after infection, NS proteins colocalized with viral DNA to form intranuclear inclusions, whereas VP...

  13. Feed efficiency and body weight growth throughout growing-furring period in mink using random regression method

    DEFF Research Database (Denmark)

    Shirali, Mahmoud; Nielsen, Vivi Hunnicke; Møller, Steen Henrik

    2014-01-01

    days of age for 2139 male mink and the same number of females. Cumulative feed intake was calculated six times with three weeks interval based on daily feed consumption between weighing’s from 105 to 210 days of age. Heritability estimates for RFI increased by age from 0.18 (0.03, standard deviation...

  14. Brains of Native and Alien Mesocarnivores in Biomonitoring of Toxic Metals in Europe.

    Directory of Open Access Journals (Sweden)

    Elzbieta Kalisinska

    Full Text Available Mercury (Hg, lead (Pb and cadmium (Cd are involved in mammalian brain damage. However, little is known about Pb and Cd brain levels in wildlife that reflect the geochemical background. The aims of the study include the estimation of Hg, Pb and Cd concentrations, and the determination of relationships between these elements in the brains of 94 mesocarnivores. Road-killed or hunted animals were obtained from north-western Poland near the Polish-German border. The investigation covered the native Eurasian otter Lutra lutra, badger Meles meles, pine marten Martes martes, beech marten M. foina, European polecat Mustela putorius, red fox Vulpes vulpes, and alien species: feral and ranch American mink Neovison vison, raccoon Procyon lotor and raccoon dog Nyctereutes procyonoides. Depending on the diet and environmental pollution, the carnivore brains accumulated toxic metals in varying amounts. The highest median Hg levels (in mg/kg dry weight, dw were found in the piscivorous Eurasian otter and feral mink (2.44 and 3.96, Pb in the omnivorous raccoon (0.47, while Cd in minks (~0.06. We indicated that Pb-based ammunition is a significant source of the element in scavengers from hunting area, and we also found a significant correlation between Pb and Cd levels in the fox brain. Finally, this study is the first to suggest background levels for brain Pb and Cd in mesocarnivores (<0.50 and <0.04 mg/kg dw, respectively.

  15. Brains of Native and Alien Mesocarnivores in Biomonitoring of Toxic Metals in Europe.

    Science.gov (United States)

    Kalisinska, Elzbieta; Lanocha-Arendarczyk, Natalia; Kosik-Bogacka, Danuta; Budis, Halina; Podlasinska, Joanna; Popiolek, Marcin; Pirog, Agnieszka; Jedrzejewska, Ewa

    2016-01-01

    Mercury (Hg), lead (Pb) and cadmium (Cd) are involved in mammalian brain damage. However, little is known about Pb and Cd brain levels in wildlife that reflect the geochemical background. The aims of the study include the estimation of Hg, Pb and Cd concentrations, and the determination of relationships between these elements in the brains of 94 mesocarnivores. Road-killed or hunted animals were obtained from north-western Poland near the Polish-German border. The investigation covered the native Eurasian otter Lutra lutra, badger Meles meles, pine marten Martes martes, beech marten M. foina, European polecat Mustela putorius, red fox Vulpes vulpes, and alien species: feral and ranch American mink Neovison vison, raccoon Procyon lotor and raccoon dog Nyctereutes procyonoides. Depending on the diet and environmental pollution, the carnivore brains accumulated toxic metals in varying amounts. The highest median Hg levels (in mg/kg dry weight, dw) were found in the piscivorous Eurasian otter and feral mink (2.44 and 3.96), Pb in the omnivorous raccoon (0.47), while Cd in minks (~0.06). We indicated that Pb-based ammunition is a significant source of the element in scavengers from hunting area, and we also found a significant correlation between Pb and Cd levels in the fox brain. Finally, this study is the first to suggest background levels for brain Pb and Cd in mesocarnivores (<0.50 and <0.04 mg/kg dw, respectively).

  16. Severity of Expert-Identified Behavioural Responses of Humpback Whale, Minke Whale, and Northern Bottlenose Whale to Naval Sonar

    NARCIS (Netherlands)

    Sivle, L.D.; Kvadsheim, P.H.; Cure, C.; Isojunno, S.; Wensveen, P.J.; Lam, F.P.A.; Visser, F.; Kleivane, L.; Tyack, P.L.; Harris, C.M.; Miller, P.J.O.

    2015-01-01

    Controlled exposure experiments using 1 to2 kHz sonar signals were conducted with 11 humpback whales (Megaptera novaeangliae), one minke whale (Balaenoptera acutorostrata), and one northern bottlenose whale (Hyperoodon ampullatus) during three field trials from 2011 to 2013. Ship approaches without

  17. Short-term effect of oral amoxicillin treatment on the gut microbial community composition in farm mink (Neovison vison)

    DEFF Research Database (Denmark)

    Marker, Laura Meier; Hammer, Anne Sofie; Andresen, Lars

    2017-01-01

    It is well documented that antibiotics have pronounced modulatory effects on the intestinal bacterial community of both humans and animals, with potential health consequences. The gut microbiota of mink has however attracted little attention due to low bacterial load and fast gastrointestinal...

  18. The effects of feed restriction on physical activity, body weight, physiology, haematology and immunology in female mink

    DEFF Research Database (Denmark)

    Damgaard, Birthe Marie; Dalgaard, Tina Sørensen; Larsen, Torben

    2012-01-01

    The aim of the present study was to investigate if adult mink females characterised as having a high or low residual feed intake (RFI) differed in their response to feed restriction with regard to activity, body weight loss and physiological parameters. For RFI-High, the activity was higher prior...

  19. Wildlife Reservoirs of Canine Distemper Virus Resulted in a Major Outbreak in Danish Farmed Mink (Neovison vison)

    DEFF Research Database (Denmark)

    Trebbien, Ramona; Chriél, Mariann; Struve, Tina

    2014-01-01

    A major outbreak of canine distemper virus (CDV) in Danish farmed mink (Neovison vison) started in the late summer period of 2012. At the same time, a high number of diseased and dead wildlife species such as foxes, raccoon dogs, and ferrets were observed. To track the origin of the outbreak virus...

  20. Identification of biosecurity measures and spatial variables as potential risk factors for Aleutian disease in Danish mink farms

    DEFF Research Database (Denmark)

    Themudo, Goncalo Espregueira Cruz; Houe, Hans; Agger, Jens Frederik Gramstrup

    2012-01-01

    Disease Virus. These factors account for 35% of the variation of the infection status of mink farms located in Vendsyssel during 2009. These results indicate that only a coordinated effort from the farmers in the area will succeed in eradicating the disease from Denmark, because individual farms that have...

  1. Eosinophilic gastroenteritis with Splendore-Hoeppli material in the ferret (Mustela putorius furo).

    Science.gov (United States)

    Fox, J G; Palley, L S; Rose, R

    1992-01-01

    Eosinophilic gastroenteritis, focal or diffuse with eosinophilic infiltrations of the stomach or intestine, has been described in human beings, cats, dogs, and horses. In this paper, we describe infiltration of the gastrointestinal tract with eosinophils accompanied by a circulating eosinophilia in six ferrets (Mustela putorius furo). Clinical signs included chronic weight loss, anorexia, and diarrhea. The small intestines from five ferrets had diffuse infiltrates of eosinophils. This resulted in focal or multifocal loss of the muscular tunic in three ferrets. Two of these ferrets also had eosinophilic gastritis. Eosinophilic granulomas with Splendore-Hoeppli material were present in mesenteric lymph nodes in four ferrets. Two ferrets had multiple organ involvement; one had eosinophilic granulomas in the liver, mesentery, and choroid plexus as well as moderate parapancreatic segmental arteritis with infiltration of eosinophils and mural thrombosis. The second ferret had, in addition to moderate diffuse gastric and small intestinal eosinophilic mucosal infiltrations, interstitial eosinophilic pulmonary infiltrates. Examination of all tissues failed to reveal an infectious agent.

  2. Multispecies functional response of the minke whale Balaenoptera acutorostrata based on small-scale foraging studies

    OpenAIRE

    Smout, Sophie; Lindstrøm, Ulf

    2007-01-01

    Atlantic minke whales are important predators in the Barents Sea ecosystem; capelin Mallotus villosus, krill Thysanoessa sp. and Meganyctephanes norvegica and herring Clupea harengus are their major prey. Their consumption of commercial species may present an economic problem for the local fishery. In order to estimate this consumption and understand the potential consequences for prey dynamics, it is essential to determine the multispecies functional response of the whales. The parameterisat...

  3. Fetal life malnutrition was not reflected in the relative abundances of adiponectin and leptin mRNAs in adipose tissue in male mink kits at 9.5 weeks of age

    DEFF Research Database (Denmark)

    Matthiesen, Connie Frank; Tauson, Anne-Helene

    2016-01-01

    Background: Malnutrition in fetal life and during suckling have in some animal studies resulted in adaptive changes related to the fat and glucose metabolism, which in the long term might predispose the offspring for metabolic disorders such as obesity later in life. The objective was to study...... the effect of fetal life malnutrition in male mink on the gene expression of leptin and adiponectin in different adipose tissue sites. Results: Thirty-two male mink, strict carnivore species, exposed to low (FL) or adequate (FA) protein provision the last 16.3 ± 1.8 days of fetal life and randomly assigned.......5 weeks of age. Relative abundances of leptin and adiponectin mRNAs were different between adipose tissue sites and were significantly higher in subcutaneous than in perirenal and mesenteric tissues. Conclusion:Fetal life protein malnutrition in male mink, did not result in adaptive changes in the gene...

  4. Utilization of milk energy by suckling mink kits

    DEFF Research Database (Denmark)

    Tauson, Anne-Helene; Fink, Rikke; Hansen, Kirsten Bislev

    2004-01-01

    A total of 36 mink dams and their litters of 3, 6 or 9 kits were used for determination of milk intake of the suckling young by means of deuterium dilution technique, and chemical composition of milk and of kit bodies. Measurements were performed during lactation weeks 1-4, each week with 3 dams...... with each litter size. Milk intake was determined over a 48 h measurement period, and by the end of this milk samples were collected and 2 kits (litters of 6 and 9) or 1 kit per litter (litters of 3) were killed for body chemical composition. Based on the results, different models were applied...... for calculation of the energetic efficiency of milk. Dam milk yield increased steadily from week 1 until week 3 but only slightly from week 3 to 4. The increase declined with increasing litter size, and for dams suckling 9 kits the increment from week 3 to week 4 was only 2 g. The dry matter content of milk...

  5. Cystic urogenital anomalies in ferrets (Mustela putorius furo).

    Science.gov (United States)

    Li, X; Fox, J G; Erdman, S E; Lipman, N S; Murphy, J C

    1996-03-01

    Single or multiple semispherical to bilobulated fluid-filled cystic structures of variable size were observed on the dorsal aspects of the urinary bladder of four male and two female ferrets (Mustela putorius furo). All ferrets had been neutered. On physical examination, the cysts were palpated as caudal abdominal masses. Three of the six ferrets presented with dysuria, and two ferrets had signs compatible with endocrine dysfunction. Adrenal cortical hyperplasia or neoplasia were observed in all of the five ferrets examined. Sex hormones assayed in one of the six ferrets revealed elevated levels of serum estrodiol. The posterior aspect of the cysts was located on and/or attached to the trigone or neck of the bladder, with variable intraluminal communication with the bladder and/or the urethra. The anterior aspect of the cysts projected dorsally or dorsocranially into the caudal abdomen. The cysts were thin walled and contained urinelike fluid (n = 5) or viscous yellow fluid (n = 1). Histologically, the cyst walls were composed of three layers, epithelium, muscle, and serosa, with fibrovascular stroma between layers. The epithelium consisted of simple to stratified transitional, columnar, or squamous epithelial cells. The muscular layer consisted of intermittent bundles and/or single to double layers of continuous to discontinuous smooth muscle. The serosal layer consisted of loose fibrous stroma covered by flattened mesothelial cells. The cystic anomalies in these ferrets were most likely derived from the urogenital glands/ducts or other remnants.

  6. Typing of Pseudomonas aeruginosa from hemorrhagic pneumonia in mink (Neovison vison)

    DEFF Research Database (Denmark)

    Salomonsen, Charlotte Mark; Themudo, G. E.; Jelsbak, Lars

    2013-01-01

    . The objective of this study was to determine the genetic and geographical relationship among outbreaks of hemorrhagic pneumonia by pulsed field gel electrophoresis typing of P. aeruginosa isolates. Furthermore, chosen isolates were typed by a commercial genotyping method based on single nucleotide polymorphisms...... (SNPs) and compared to a larger dataset of human and environmental origin. The bacterial isolates were obtained from diagnostic samples from 2002-2009 and contained 164 isolates from 95 outbreaks on 90 farms. Our results show that most outbreaks of hemorrhagic pneumonia in mink are caused by distinct...

  7. Thermal adaptation of the crucian carp (Carassius carassius) cardiac delayed rectifier current, IKs, by homomeric assembly of Kv7.1 subunits without MinK.

    Science.gov (United States)

    Hassinen, Minna; Laulaja, Salla; Paajanen, Vesa; Haverinen, Jaakko; Vornanen, Matti

    2011-07-01

    Ectothermic vertebrates experience acute and chronic temperature changes which affect cardiac excitability and may threaten electrical stability of the heart. Nevertheless, ectothermic hearts function over wide range of temperatures without cardiac arrhythmias, probably due to special molecular adaptations. We examine function and molecular basis of the slow delayed rectifier K(+) current (I(Ks)) in cardiac myocytes of a eurythermic fish (Carassius carassius L.). I(Ks) is an important repolarizing current that prevents excessive prolongation of cardiac action potential, but it is extremely slowly activating when expressed in typical molecular composition of the endothermic animals. Comparison of the I(Ks) of the crucian carp atrial myocytes with the currents produced by homomeric K(v)7.1 and heteromeric K(v)7.1/MinK channels in Chinese hamster ovary cells indicates that activation kinetics and pharmacological properties of the I(Ks) are similar to those of the homomeric K(v)7.1 channels. Consistently with electrophysiological properties and homomeric K(v)7.1 channel composition, atrial transcript expression of the MinK subunit is only 1.6-1.9% of the expression level of the K(v)7.1 subunit. Since activation kinetics of the homomeric K(v)7.1 channels is much faster than activation of the heteromeric K(v)7.1/MinK channels, the homomeric K(v)7.1 composition of the crucian carp cardiac I(Ks) is thermally adaptive: the slow delayed rectifier channels can open despite low body temperatures and curtail the duration of cardiac action potential in ectothermic crucian carp. We suggest that the homomeric K(v)7.1 channel assembly is an evolutionary thermal adaptation of ectothermic hearts and the heteromeric K(v)7.1/MinK channels evolved later to adapt I(Ks) to high body temperature of endotherms.

  8. The mediator-modulator brain system in minks exposed to radiation factors of the Chernobyl NPP 10-km zone

    International Nuclear Information System (INIS)

    Mishunina, T.M.; Kalinskaya, L.N.; Pil'kevich, L.I.; Kononenko, V.Ya.; Ryasenko, V.I.; Bogdanova, T.I.

    1996-01-01

    The paper is devoted to the study of the activity of the important metabolizing mediators/modulators of the central nervous system: adenosine, angiotensin and GABA as well as specific bonding of 14 C-GABA by synaptic membranes of brain structures in minks who were in the 10-km zone of the Chernobyl NPP from 0.5 months to 3 years. Adenosine deaminase, angiotensin converting enzyme and glutamate decarboxylase activities endured significant changes in the hypothalamus, striatum, hippocampus, cerebellum, cerebral, cortex, medulla oblongata and midbrain of minks; the direction and the extent of manifestation of those changes depend on the term of animals' stay in the zone. The GABA reception in the cerebral cortex and medulla oblongata increased significantly and progressively with the prolongation of exposure to radiation. It is concluded that the variety of changes, their regional-specificity and dependence on exposure of animals to the radiation factors of the Chernobyl NPP 10-km zone may induce development of serious complications not only in nervous but in some other systems

  9. Rapid development of fasting-induced hepatic lipidosis in the American mink (Neovison vison): effects of food deprivation and re-alimentation on body fat depots, tissue fatty acid profiles, hematology and endocrinology.

    Science.gov (United States)

    Rouvinen-Watt, Kirsti; Mustonen, Anne-Mari; Conway, Rebecca; Pal, Catherine; Harris, Lora; Saarela, Seppo; Strandberg, Ursula; Nieminen, Petteri

    2010-02-01

    Hepatic lipidosis is a common pathological finding in the American mink (Neovison vison) and can be caused by nutritional imbalance due to obesity or rapid body weight loss. The objectives of the present study were to investigate the timeline and characterize the development of hepatic lipidosis in mink in response to 0-7 days of food deprivation and liver recovery after 28 days of re-feeding. We report here the effects on hematological and endocrine variables, body fat mobilization, the development of hepatic lipidosis and the alterations in the liver lipid classes and tissue fatty acid (FA) sums. Food deprivation resulted in the rapid mobilization of body fat, most notably visceral, causing elevated hepatosomatic index and increased liver triacylglycerol content. The increased absolute amounts of liver total phospholipids and phosphatidylcholine suggested endoplasmic reticulum stress. The hepatic lipid infiltration and the altered liver lipid profiles were associated with a significantly reduced proportion of n-3 polyunsaturated FA (PUFA) in the livers and the decrease was more evident in the females. Likewise, re-feeding of the female mink resulted in a more pronounced recovery of the liver n-3 PUFA. The rapid decrease in the n-3/n-6 PUFA ratio in response to food deprivation could trigger an inflammatory response in the liver. This could be a key contributor to the pathophysiology of fatty liver disease in mink influencing disease progression.

  10. De novo SNP calling from RAD sequences for a mink (Neovison vison) specific genotyping assay

    DEFF Research Database (Denmark)

    Thirstrup, Janne Pia; Pujolar, José Martin; Larsen, Peter F

    2013-01-01

    mink from Brown and Black color types were obtained. A mean of 49,789,860.2 (± 9,813,587.2) raw reads of high quality per sample were sequenced. SNPs were called using the software pipeline Stacks. The populations program was used to estimate population structure and genetic divergence between the two...

  11. A large insertion in intron 2 of the TYRP1 gene associated with American Palomino phenotype in American mink

    DEFF Research Database (Denmark)

    Cirera Salicio, Susanna; Markakis, Marios Nektarios; Kristiansen, Thea

    2016-01-01

    A number of American mink phenotypes display a range of brownish colours. One of these phenotypes, namely American Palomino (b (P) b (P) ) (AP) has been found to be associated with the tyrosinase-related protein 1 (TYRP1) gene by genotyping microsatellite markers in one sire family. Trials for am...... and the same size insertion appears to be present. By this we suggest that certain modifiers of TYRP1 would induce different brown colour degradation, which results in at least two different phases of brown.......A number of American mink phenotypes display a range of brownish colours. One of these phenotypes, namely American Palomino (b (P) b (P) ) (AP) has been found to be associated with the tyrosinase-related protein 1 (TYRP1) gene by genotyping microsatellite markers in one sire family. Trials...

  12. Outbreak tracking of Aleutian mink disease virus (AMDV) using partial NS1 gene sequencing

    DEFF Research Database (Denmark)

    Ryt-Hansen, Pia; Hjulsager, Charlotte Kristiane; Hagberg, E. E.

    2017-01-01

    . However, in 2015, several outbreaks of AMDV occurred at mink farms throughout Denmark, and the sources of these outbreaks were not known. Partial NS1 gene sequencing, phylogenetic analyses data were utilized along with epidemiological to determine the origin of the outbreaks. The phylogenetic analyses...... not be excluded. This study confirmed that partial NS1 sequencing can be used in outbreak tracking to determine major viral clusters of AMDV. Using this method, two new distinct AMDV clusters with low intra-cluster sequence diversity were identified, and epidemiological data helped to reveal possible ways...

  13. A probabilistic risk assessment of the effects of methylmercury and PCBs on mink and kingfishers along East Fork Poplar Creek, Oak Ridge, Tennessee, USA

    Energy Technology Data Exchange (ETDEWEB)

    Moore, D.R.J.; Sample, B.E.; Suter, G.W.; Parkhurst, B.R.; Teed, R.S.

    1999-12-01

    Over fifty years of operations, storage, and disposal of wastes from the US Department of Energy (US DOE) Y-12 nuclear weapons facility at Oak Ridge, Tennessee, USA, has resulted in the contamination of water, sediment, biota, and floodplain soils of East Fork Poplar Creek. A preliminary assessment revealed that methylmercury and polychlorinated biphenyls (PCBs) were the contaminants of most concern. Because these contaminants are persistent, accumulate in tissues, and biomagnify up the food chain, piscivorous wildlife are the biota at greatest risk of exposure. The objective of this study was to estimate the risks posed by methylmercury and PCBs to two piscivorous species: mink and belted kingfishers. The authors conducted Monte Carlo simulations to estimate total daily intakes of each contaminant by each species and then integrated the resulting distributions with their respective dose-response curves to estimate risks. The results indicate that methylmercury poses a moderate risk to female mink (24% probability of at least 15% mortality) and kingfishers (50% probability of at least a 12--28% decline in fecundity depending on location). The PCBs pose a very serious risk to mink (52% probability of at least a 50% decline in reproductive fecundity), a species known to be especially sensitive to the effects of organochlorine substances, but little risk to kingfishers (<5% probability of a decline in reproductive fecundity greater than 10% at any location).

  14. The Ste20 Family Kinases MAP4K4, MINK1, and TNIK Converge to Regulate Stress-Induced JNK Signaling in Neurons.

    Science.gov (United States)

    Larhammar, Martin; Huntwork-Rodriguez, Sarah; Rudhard, York; Sengupta-Ghosh, Arundhati; Lewcock, Joseph W

    2017-11-15

    The c-Jun- N -terminal kinase (JNK) signaling pathway regulates nervous system development, axon regeneration, and neuronal degeneration after acute injury or in chronic neurodegenerative disease. Dual leucine zipper kinase (DLK) is required for stress-induced JNK signaling in neurons, yet the factors that initiate DLK/JNK pathway activity remain poorly defined. In the present study, we identify the Ste20 kinases MAP4K4, misshapen-like kinase 1 (MINK1 or MAP4K6) and TNIK Traf2- and Nck-interacting kinase (TNIK or MAP4K7), as upstream regulators of DLK/JNK signaling in neurons. Using a trophic factor withdrawal-based model of neurodegeneration in both male and female embryonic mouse dorsal root ganglion neurons, we show that MAP4K4, MINK1, and TNIK act redundantly to regulate DLK activation and downstream JNK-dependent phosphorylation of c-Jun in response to stress. Targeting MAP4K4, MINK1, and TNIK, but not any of these kinases individually, is sufficient to protect neurons potently from degeneration. Pharmacological inhibition of MAP4Ks blocks stabilization and phosphorylation of DLK within axons and subsequent retrograde translocation of the JNK signaling complex to the nucleus. These results position MAP4Ks as important regulators of the DLK/JNK signaling pathway. SIGNIFICANCE STATEMENT Neuronal degeneration occurs in disparate circumstances: during development to refine neuronal connections, after injury to clear damaged neurons, or pathologically during disease. The dual leucine zipper kinase (DLK)/c-Jun- N -terminal kinase (JNK) pathway represents a conserved regulator of neuronal injury signaling that drives both neurodegeneration and axon regeneration, yet little is known about the factors that initiate DLK activity. Here, we uncover a novel role for a subfamily of MAP4 kinases consisting of MAP4K4, Traf2- and Nck-interacting kinase (TNIK or MAP4K7), and misshapen-like kinase 1 (MINK1 or MAP4K6) in regulating DLK/JNK signaling in neurons. Inhibition of

  15. Development of a PCR-RFLP assay for the detection and differentiation of canine parvovirus and mink enteritis virus.

    Science.gov (United States)

    Zhang, Chuanmei; Yu, Yongle; Yang, Haiyan; Li, Guimei; Yu, Zekun; Zhang, Hongliang; Shan, Hu

    2014-12-15

    A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay has been developed to detect and differentiate between canine parvovirus (CPV) and mink enteritis virus (MEV). Eight CPV and three MEV epidemic strains isolated from 28 pathological samples from dogs and minks suspected of being infected with parvovirus were amplified by PCR using a pair of specific primers designed based on the CPV-N strain (M19296). PCR amplified a fragment of 1016bp from the genomic DNA of both MEV and CPV. The MEV-derived fragment could be digested with the restriction enzyme BSP1407I into three fragments of 102bp, 312bp and 602bp, while the fragment amplified from the CPV genomic DNA was digested into only two fragments of 414bp and 602bp. The lowest DNA concentration of CPV and MEV that could be detected using this assay was 0.004μg/ml and 0.03μg/ml, respectively. The PCR-RFLP assay developed in the present study can, therefore, be used to detect and differentiate MEV from CPV with high specificity and sensitivity. Copyright © 2014 Elsevier B.V. All rights reserved.

  16. Garbage in the diet of carnivores in an agricultural area

    Directory of Open Access Journals (Sweden)

    Jankowiak Łukasz

    2016-06-01

    Full Text Available Human food waste is considered to be richer in carbohydrates, lipids and proteins than most natural food supplies; however, it is very well digested in scats. So, as an indication of this kind of food in the diet, we have used each indigestible, anthropogenic origin element found in faeces (e.g., glass, plastic, rubber, etc.. There are few studies discussing the importance of garbage in the diet of mammalian predators living in farmland; definitely, most focus on this issue in urban areas. We studied the contribution of garbage in the diet of raccoon dog (Nyctereutes procyonoides, red fox (Vulpes vulpes, marten (Martes sp., polecat (Mustela putorius, stoat (Mustela erminea, American mink (Neovison vison and Eurasian otter (Lutra lutra in the agricultural areas of western Poland in 2006-2010. In addition, we examined the spatial changes in the diet of red fox and polecat. The largest contribution of garbage was found in scats of raccoon dog (8.8%, red fox (4.8% and marten (4.3%. The diet of polecat, stoat and Eurasian otter contained 2.5%, 1.7% and 0.2% garbage items respectively. The most frequent item was plastic. Our analysis showed that garbage consumption by red fox and polecat was greater closer to human settlements. The results reveal a continuous gradient in the garbage consumption that corresponds with the degree of synanthropization of particular species.

  17. Extreme Tetralogy of Fallot With Polycythemia in a Ferret (Mustela putorius furo).

    Science.gov (United States)

    Dias, Sara; Planellas, Marta; Canturri, Albert; Martorell, Jaume

    2017-06-01

    A 5-month-old, intact male ferret (Mustela putorius furo) was presented with apathy, tachypnea and exercise intolerance. On initial physical examination, tachypnea and cyanosis were the two primary clinical signs detected. The complete blood count also revealed a severe polycythemia, with lymphopenia, neutrophilia and eosinophilia. Further diagnostic imaging tests were performed. The thoracic radiographs revealed cardiomegaly and a diffuse alveolar pulmonary pattern. Consequently, an echocardiography was performed and showed an interventricular septal defect, pulmonic artery occlusion, overriding of the aorta and right ventricle hypertrophy. All abnormalities were compatible with a tetralogy of Fallot (TOF). As a treatment plan, a phlebotomy was performed and the ferret was posteriorly maintained with oxygen and fluid therapy. The following day, the hematocrit decreased 11% and the respiratory distress improved. Four days later, the ferret got clinically worse and was euthanized. A necropsy was performed and confirmed the presence of an extreme TOF with a diffused interstitial pneumonia secondary to Pneumocystis carinii. To the author's knowledge, this is the first report of an extreme tetralogy of Fallot with polycythemia in a ferret. Copyright © 2017 Elsevier Inc. All rights reserved.

  18. Analysis of Factors Influencing Fur Quality in Minks of Standard, Pastel, Platinum and White Hedlunda Colour Strains

    OpenAIRE

    Stanisław Socha; Dorota Kołodziejczyk; Ewa Kondraciuk; Danuta Wójcik; Aldona Gontarz

    2010-01-01

    The work aimed at the analysis of the factors that influence conformation traits, included animal size and fur quality traits in four colour types of mink: standard, pastel, platinum and white Hedlunda. The data concerns the evaluation of animal conformation traits in the period of three years. The analysis of variance of particular traits indicates statistically significant effect of the year of birth, colour type and animal sex on the majority of analysed traits. Higher means of license eva...

  19. Effects of protein restriction in utero on the metabolism of mink dams (Neovison vison) and on mink kit survival as well as on postnatal growth

    DEFF Research Database (Denmark)

    Vesterdorf, Kristine Høvelt; Harrison, Adrian Paul; Matthiesen, Connie Marianne Frank

    2012-01-01

    be determined. Mink dams were fed an adequate protein (AP; crude protein:fat:carbo- hydrate ratio of 31:55:14% of metabolizable energy, ME) or a low protein diet (LP; 19%:49%: 32% of ME) during the last 21.2 ± 3.3 days of gestation, followed by an adequate diet during lactation. Respiration and balance...... experiments were performed during late gestation and twice during lactation. The dietary treatment only affected energy metabolism traits significantly during the treatment period in late gestation, such that LP dams oxidized less protein (12% vs 23% of heat production, HE, P = 0.001) but more carbohydrate...... (37% vs 26% of HE, P vs 0.4 g.kg-0.75.day-1, P vs 1.4, P vs 11.6 g...

  20. Model of the PCB and mercury exposure of mink and great blue heron inhabiting the off-site environment downstream from the US Department of Energy Oak Ridge Reservation

    International Nuclear Information System (INIS)

    MacIntosh, D.L.

    1992-09-01

    This report presents a pair of wildlife exposure models developed for use in investigating the risks to wildlife of releases of mercury and PCBS. The species modeled are the great blue heron and mink The models may be used to estimate the exposure experienced by mink and herons, to help establish remedial action goals and to identify research needs. Because mercury and PCBs bioaccumulate through dietary uptake, the models simulate the food webs supporting the two species. Sources of contaminants include surface water, sediment, sediment pore water, and soil. The model are stochastic equilibrium models. Two types of variance in the input parameters are distinguished: stochastic variance among individual mink and herons and ignorance concerning true parameter values. The variance in the output due to stochastic parameters indicates the expected variance among the receptors. The variance due to ignorance indicates the extent to which the model outputs could be unpaved by additional sampling and measurement. The results of the models were compared to concentrations measured in great blue heron eggs and nestlings from colonies on the Clinch and Tennessee Rivers. The predicted concentrations agreed well with the measured concentrations. In addition, the variances in measured values among individuals was approximately equal to the total stochastic variance predicted by the models

  1. Model of the PCB and mercury exposure of mink and great blue heron inhabiting the off-site environment downstream from the US Department of Energy Oak Ridge Reservation

    Energy Technology Data Exchange (ETDEWEB)

    MacIntosh, D.L. (Indiana Univ., Bloomington, IN (United States). School of Public and Environmental Affairs); Suter, G.W. II; Hoffman, F.O. (Oak Ridge National Lab., TN (United States))

    1992-09-01

    This report presents a pair of wildlife exposure models developed for use in investigating the risks to wildlife of releases of mercury and PCBS. The species modeled are the great blue heron and mink The models may be used to estimate the exposure experienced by mink and herons, to help establish remedial action goals and to identify research needs. Because mercury and PCBs bioaccumulate through dietary uptake, the models simulate the food webs supporting the two species. Sources of contaminants include surface water, sediment, sediment pore water, and soil. The model are stochastic equilibrium models. Two types of variance in the input parameters are distinguished: stochastic variance among individual mink and herons and ignorance concerning true parameter values. The variance in the output due to stochastic parameters indicates the expected variance among the receptors. The variance due to ignorance indicates the extent to which the model outputs could be unpaved by additional sampling and measurement. The results of the models were compared to concentrations measured in great blue heron eggs and nestlings from colonies on the Clinch and Tennessee Rivers. The predicted concentrations agreed well with the measured concentrations. In addition, the variances in measured values among individuals was approximately equal to the total stochastic variance predicted by the models.

  2. Estimation of indirect genetic effects in group-housed mink (Neovison vison) should account for systematic interactions either due to kin or sex

    DEFF Research Database (Denmark)

    Alemu, Setegn Worku; Berg, Peer; Janss, Luc

    2016-01-01

    interactions in group-housed mink. Furthermore, we investigated whether systematic non-genetic interactions between kin or individuals of the same sex influence the estimates of genetic parameters. As a second objective, we clarify the relationship between estimates of the traditional IGE model and a family...

  3. The auditory anatomy of the minke whale (Balaenoptera acutorostrata): a potential fatty sound reception pathway in a baleen whale.

    Science.gov (United States)

    Yamato, Maya; Ketten, Darlene R; Arruda, Julie; Cramer, Scott; Moore, Kathleen

    2012-06-01

    Cetaceans possess highly derived auditory systems adapted for underwater hearing. Odontoceti (toothed whales) are thought to receive sound through specialized fat bodies that contact the tympanoperiotic complex, the bones housing the middle and inner ears. However, sound reception pathways remain unknown in Mysticeti (baleen whales), which have very different cranial anatomies compared to odontocetes. Here, we report a potential fatty sound reception pathway in the minke whale (Balaenoptera acutorostrata), a mysticete of the balaenopterid family. The cephalic anatomy of seven minke whales was investigated using computerized tomography and magnetic resonance imaging, verified through dissections. Findings include a large, well-formed fat body lateral, dorsal, and posterior to the mandibular ramus and lateral to the tympanoperiotic complex. This fat body inserts into the tympanoperiotic complex at the lateral aperture between the tympanic and periotic bones and is in contact with the ossicles. There is also a second, smaller body of fat found within the tympanic bone, which contacts the ossicles as well. This is the first analysis of these fatty tissues' association with the auditory structures in a mysticete, providing anatomical evidence that fatty sound reception pathways may not be a unique feature of odontocete cetaceans. Copyright © 2012 Wiley Periodicals, Inc.

  4. The failure of an inactivated mink enteritis virus vaccine in four preparations to provide protection to dogs against challenge with canine parvovirus-2.

    OpenAIRE

    Carman, S; Povey, C

    1982-01-01

    Four experimental vaccine preparations comprising a strain of mink enteritis virus inactivated by either formalin or beta-propiolactone, and either adjuvanted or nonadjuvanted, failed to stimulate a consistent serum antibody response in 20 vaccinated dogs and failed to protect all but one of these dogs against oral challenge with canine parvovirus-2.

  5. Feeding mink (Neovison vison) a protein-restricted diet during pregnancy induces higher birth weight and altered hepatic gene expression in the F2 offspring

    DEFF Research Database (Denmark)

    Matthiesen, Connie Marianne Frank; Blache, Dominique; Thomsen, Preben Dybdahl

    2010-01-01

    Malnutrition during foetal life can induce modifications in the phenotype of an individual. The present study aimed to observe effects of low foetal life protein provision on modifications of the phenotype and changes in the progeny of 1-year-old female mink (F1 generation) offspring of mothers f...

  6. Longitudinal Analysis of Residual Feed Intake in Mink using Random Regression with Heterogeneous Residual Variance

    DEFF Research Database (Denmark)

    Shirali, Mahmoud; Nielsen, Vivi Hunnicke; Møller, Steen Henrik

    Heritability of residual feed intake (RFI) increased from low to high over the growing period in male and female mink. The lowest heritability for RFI (male: 0.04 ± 0.01 standard deviation (SD); female: 0.05 ± 0.01 SD) was in early and the highest heritability (male: 0.33 ± 0.02; female: 0.34 ± 0.......02 SD) was achieved at the late growth stages. The genetic correlation between different growth stages for RFI showed a high association (0.91 to 0.98) between early and late growing periods. However, phenotypic correlations were lower from 0.29 to 0.50. The residual variances were substantially higher...

  7. The draft genome sequence of the ferret (Mustela putorius furo) facilitates study of human respiratory disease.

    Science.gov (United States)

    Peng, Xinxia; Alföldi, Jessica; Gori, Kevin; Eisfeld, Amie J; Tyler, Scott R; Tisoncik-Go, Jennifer; Brawand, David; Law, G Lynn; Skunca, Nives; Hatta, Masato; Gasper, David J; Kelly, Sara M; Chang, Jean; Thomas, Matthew J; Johnson, Jeremy; Berlin, Aaron M; Lara, Marcia; Russell, Pamela; Swofford, Ross; Turner-Maier, Jason; Young, Sarah; Hourlier, Thibaut; Aken, Bronwen; Searle, Steve; Sun, Xingshen; Yi, Yaling; Suresh, M; Tumpey, Terrence M; Siepel, Adam; Wisely, Samantha M; Dessimoz, Christophe; Kawaoka, Yoshihiro; Birren, Bruce W; Lindblad-Toh, Kerstin; Di Palma, Federica; Engelhardt, John F; Palermo, Robert E; Katze, Michael G

    2014-12-01

    The domestic ferret (Mustela putorius furo) is an important animal model for multiple human respiratory diseases. It is considered the 'gold standard' for modeling human influenza virus infection and transmission. Here we describe the 2.41 Gb draft genome assembly of the domestic ferret, constituting 2.28 Gb of sequence plus gaps. We annotated 19,910 protein-coding genes on this assembly using RNA-seq data from 21 ferret tissues. We characterized the ferret host response to two influenza virus infections by RNA-seq analysis of 42 ferret samples from influenza time-course data and showed distinct signatures in ferret trachea and lung tissues specific to 1918 or 2009 human pandemic influenza virus infections. Using microarray data from 16 ferret samples reflecting cystic fibrosis disease progression, we showed that transcriptional changes in the CFTR-knockout ferret lung reflect pathways of early disease that cannot be readily studied in human infants with cystic fibrosis disease.

  8. Model of the PCB and mercury exposure of mink and great blue heron inhabiting the off-site environment downstream from the US Department of Energy Oak Ridge Reservation. Environmental Restoration Program

    Energy Technology Data Exchange (ETDEWEB)

    MacIntosh, D.L. [Indiana Univ., Bloomington, IN (United States). School of Public and Environmental Affairs; Suter, G.W. II; Hoffman, F.O. [Oak Ridge National Lab., TN (United States)

    1992-09-01

    This report presents a pair of wildlife exposure models developed for use in investigating the risks to wildlife of releases of mercury and PCBS. The species modeled are the great blue heron and mink The models may be used to estimate the exposure experienced by mink and herons, to help establish remedial action goals and to identify research needs. Because mercury and PCBs bioaccumulate through dietary uptake, the models simulate the food webs supporting the two species. Sources of contaminants include surface water, sediment, sediment pore water, and soil. The model are stochastic equilibrium models. Two types of variance in the input parameters are distinguished: stochastic variance among individual mink and herons and ignorance concerning true parameter values. The variance in the output due to stochastic parameters indicates the expected variance among the receptors. The variance due to ignorance indicates the extent to which the model outputs could be unpaved by additional sampling and measurement. The results of the models were compared to concentrations measured in great blue heron eggs and nestlings from colonies on the Clinch and Tennessee Rivers. The predicted concentrations agreed well with the measured concentrations. In addition, the variances in measured values among individuals was approximately equal to the total stochastic variance predicted by the models.

  9. Characterization of the env gene and long terminal repeat of molecularly cloned Friend mink cell focus-inducing virus DNA.

    OpenAIRE

    Adachi, A; Sakai, K; Kitamura, N; Nakanishi, S; Niwa, O; Matsuyama, M; Ishimoto, A

    1984-01-01

    The highly oncogenic erythroleukemia-inducing Friend mink cell focus-inducing (MCF) virus was molecularly cloned in phage lambda gtWES.lambda B, and the DNA sequences of the env gene and the long terminal repeat were determined. The nucleotide sequences of Friend MCF virus and Friend spleen focus-forming virus were quite homologous, supporting the hypothesis that Friend spleen focus-forming virus might be generated via Friend MCF virus from an ecotropic Friend virus mainly by some deletions. ...

  10. Development of survival skills in captive-raised Siberian polecats (Mustela eversmanni) II: predator avoidance

    Science.gov (United States)

    Miller, Brian; Biggins, Dean; Wemmer, Chris; Powell, Roger; Calvo, Lorena; Hanebury, Lou; Wharton, Tracy

    1990-01-01

    We exposed naive Siberain polecats (Mustela eversmanni) (aged 2, 3, and 4 months) to a swooping stuffed great horned owl (Buho virginianus) and a stuffed badger (Taxidae taxus) mounted on a remote control toy automobile frame. The first introduction to each was harmless, the second was accompanied by a mild aversive stimulus, the third (1 day after attack) was harmless, and the fourth (30 days after attack) was harmless. Alert behavior increased after a single attack by either predator model. Escape responses of naive polecats did not differ between ages when exposed to the badger, but 4 month old polecats reduced their escape times after a single badger attack. When exposed to the swooping owl, naive 4 month old polecats redponded more quickly than the other two age groups, and 3 and 4 month old polecats reduced escape times after a single owl attack. This indicates an innate escape response to the owl model at 4 months of age, and a short-tert ability to remember a single mild aversive encounter with the badger and owl models at 3 or 4 months of age.

  11. Novel sequence types of extended-spectrum and acquired AmpC beta-lactamase producing Escherichia coli and Escherichia clade V isolated from wild mammals.

    Science.gov (United States)

    Alonso, Carla Andrea; Alcalá, Leticia; Simón, Carmen; Torres, Carmen

    2017-08-01

    The closer contact with wildlife due to the growing human population and the destruction of natural habitats emphasizes the need of gaining insight into the role of animals as source of antimicrobial resistance. Here, we aim at characterizing the antimicrobial resistance genes and phylogenetic distribution of commensal Escherichia coli from 62 wild mammals. Isolates exhibiting resistance to ≥1 antibiotic were detected in 25.8% of the animals and 6.4% carried an extended-spectrum beta-lactamase (ESBL)/AmpC-producing E. coli. Genetic mechanisms involved in third-generation cephalosporin resistance were as follows: (i) hyperproduction of chromosomal AmpC (hedgehog), (ii) production of acquired CMY-2 β-lactamase (hedgehog), (iii) production of SHV-12 and CTX-M-14 ESBLs (n = 2, mink and roe-deer). ESBL genes were transferable by conjugation, and blaCMY-2 was mobilized by a 95kb IncI1 plasmid. The distribution of the phylogenetic groups in the E. coli collection studied was B1 (44.6%), B2 (24.6%), E (15.4%), A (4.6%) and F (3.1%). Five isolates (7.7%) were cryptic Escherichia clades (clade IV, 4 mice; clade V, 1 mink). ESBL/AmpC-E. coli isolates showed different sequence types (STs): ST1128/B1, ST4564/B1 (new), ST4996/B1 (new) and a non-registered ST. This study contributes to better understand the E. coli population and antimicrobial resistance flow in wildlife and reports new AmpC-E. coli STs and a first described ESBL-producing Escherichia clade V isolate. © FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  12. Chylous ascites associated with abdominal trauma and intestinal resection-anastomosis in a pet ferret (Mustela putorius furo).

    Science.gov (United States)

    Chassang, Lucile; Langlois, Isabelle; Loos, Pauline; Freire, Mila; O'Toole, Elizabeth

    2018-05-15

    CASE DESCRIPTION A 10-week-old 0.73-kg (1.6-lb) castrated male domestic ferret (Mustela putorius furo) was referred for exploratory laparotomy because of pneumoperitoneum and possible septic peritonitis after being bitten by the owner's dog. CLINICAL FINDINGS Abdominal exploration revealed a large laceration of the duodenum, tears of the jejunal mesentery, and 2 small tears in the abdominal wall. Chylous abdominal effusion developed 48 hours after surgery. TREATMENT AND OUTCOME Postoperative care included supportive treatment, analgesia, and antimicrobials. An abdominal drain was placed during the laparotomy and enabled monitoring of abdominal fluid production. Enteral feeding was provided through an esophagostomy tube. The chylous fluid production rapidly decreased after treatment with octreotide was initiated, and the ferret improved. Chyloabdomen resolved after 8 days of hospitalization and medical treatment. CLINICAL RELEVANCE Findings suggested that chylous ascites can potentially develop secondary to blunt abdominal trauma in ferrets. In this ferret, chyloabdomen was successfully treated with octreotide administration and abdominal drainage.

  13. Reading into lesser bibliography of rare mustelids (Eastern Europe

    Directory of Open Access Journals (Sweden)

    Jerzy Romanowski

    1995-12-01

    Full Text Available Abstract Many research and conservation efforts on rare mustelids in Eastern Europe, Russia and states of CIS are in progress. On a total of 640 publications examined, the otter (428 and the European mink (195 were the most studied species, the Steppe and Marbled polecats (89 and 83 entries, and Honey-badger (24 were less considered. Titles written in national languages dominate, but a recent increase in the ratio of publications written or summarized in English or German was noted. Based on number and profile of publications, it is suggested that conservation of European mink requires more efforts and research. Riassunto Bibliografia poco nota sui Mustelidi rari (Europa orientale - Nei paesi dell'Europa dell'Est, Russia e stati del CIS inclusi, le ricerche e le iniziative di conservazione riguardanti i mustelidi rari sono in progresso. Dall'esame di 640 pubblicazioni, Lutra lutra (428 e Mustela lutreola (195 sono le specie più studiate, mentre meno considerate sono M. erversmanni (89, Vormela peregusna (83 e Mellivora capensis (24. Buona parte dei lavori è in lingua originale, ma recentemente è in incremento il numero di pubblicazioni scritte o riassunte in inglese o tedesco. Sulla base del numero dei lavori e dei temi trattati, si evidenzia che la conservazione del visone europeo richiederebbe maggiori sforzi e ricerche.

  14. Environmental disturbance and conservation of marine and shoreline birds on the west coast of Vancouver Island

    International Nuclear Information System (INIS)

    Morgan, K.H.; Butler, R.W.; Vermeer, R.W.

    1992-01-01

    Loss of habitat and oiling of birds represent two major threats to marine and shoreline bird populations on Vancouver Island's west coast, since their effects are widespread and cumulative. Offshore tanker traffic and local inshore shipments of petroleum products expose the coast to high risks of oiling. Large numbers of birds are most at risk when concentrated in relatively small areas, such as highly productive feeding areas, at communal roosting sites, and around nesting colonies. Logging of mature and old-growth forests has led to destruction of the nesting habitat of marbled murrelets (Brachyramphus marmoratus), while industrial development of estuaries, mudflats, and spawning grounds of Pacific herring (Clupea harengus pallasi) has diminished feeding habitats for other marine and shoreline birds. Fisheries operations, human disturbance of colonies, and introduced predators, notably the raccoon (Procyon lotor) and mink (Mustela vison), have impacted upon local populations. Management actions and research needs to mitigate these threats are addressed. 40 refs

  15. Red River Wildlife Management Area HEP Report, Habitat Evaluation Procedures, Technical Report 2004.

    Energy Technology Data Exchange (ETDEWEB)

    Ashley, Paul

    2004-11-01

    A habitat evaluation procedures (HEP) analysis conducted on the 314-acre Red River Wildlife Management Area (RRWMA) managed by the Idaho Department of Fish and Game resulted in 401.38 habitat units (HUs). Habitat variables from six habitat suitability index (HSI) models, comprised of mink (Mustela vison), mallard (Anas platyrhynchos), common snipe (Capella gallinago), black-capped chickadee (Parus altricapillus), yellow warbler (Dendroica petechia), and white-tailed deer (Odocoileus virginianus), were measured by Regional HEP Team (RHT) members in August 2004. Cover types included wet meadow, riverine, riparian shrub, conifer forest, conifer forest wetland, and urban. HSI model outputs indicate that the shrub component is lacking in riparian shrub and conifer forest cover types and that snag density should be increased in conifer stands. The quality of wet meadow habitat, comprised primarily of introduced grass species and sedges, could be improved through development of ephemeral open water ponds and increasing the amount of persistent wetland herbaceous vegetation e.g. cattails (Typha spp.) and bulrushes (Scirpus spp.).

  16. Altered vitamin D status in liver tissue and blood plasma from Greenland sledge dogs (Canis familiaris) dietary exposed to organohalogen contaminated minke whale (Balaenoptera acuterostrata) blubber

    DEFF Research Database (Denmark)

    Sonne, Christian; Kirkegaard, Maja; Jakobsen, Jette

    2014-01-01

    This study compared vitamin D3 (vitD3) and 25-OH vitamin D3 (25OHD3) status in Greenland sledge dogs (Canis familiaris) given either minke whale (Balaenoptera acuterostrata) blubber high in organohalogen contaminants (OHCs) or clean porcine (Suis scrofa) fat for up to 636 days. A group of six...

  17. Heterosis in the second and third generation affects litter size in a crossbreed mink (Neovison vison) population

    DEFF Research Database (Denmark)

    Thirstrup, Janne Pia; Pertoldi, Cino; Larsen, Peter Foged

    2014-01-01

    Litter sizes in a cross between Brown and Black mink color types were observed through six generations. Litter size was significantly affected by yearly environmental variations. After adjusting for year effects, we found significant increases in litter size in the second and third generations (F2...... caused by an increase in litter size compared to the Black parental line. This indicates that the Black line was affected by inbreeding depression prior to crossing. We also found that two-year old F1 females had significantly more offspring compared to one-year old F1 females...

  18. Diagnosis and Management of Polycythemia Vera in a Ferret (Mustela putorius furo).

    Science.gov (United States)

    Le, Kim; Beaufrère, Hugues; Bassel, Laura L; Wills, Sarah; Laniesse, Delphine; Blois, Shauna L; Smith, Dale A

    2016-12-01

    A 5-y-old female ferret (Mustela putorius furo) was evaluated for diarrhea, anorexia, and lethargy for 1 wk. Only mild dehydration was detected on physical examination. CBC analysis revealed marked erythrocytosis with an unremarkable plasma biochemistry panel; follow-up CBC analyses revealed a consistent primary erythrocytosis. Whole-body radiographs and abdominal ultrasonography were unremarkable except for a small nephrolith in the right kidney and a small cyst in the left kidney. The plasma erythropoietin level was 17.0 mIU/mL and considered normal. In light of the diagnostic work-up and consistent erythrocytosis, a diagnosis of polycythemia vera (primary erythrocytosis) was made. The initial presentation of diarrhea resolved after treatment with oral metronidazole (20 mg/kg PO BID for 7 d). Treatment for the polycythemia consisted of a phlebotomy initially followed by chemotherapy with hydroxyurea (10 mg/kg PO BID). During the subsequent 12 mo, the hydroxyurea dose adjusted according to follow-up CBC results, and finding an optimal dosage regimen proved to be challenging. One year after the initial diagnosis, the ferret presented to an emergency clinic for acute and severe hemorrhagic diarrhea and died shortly thereafter. The postmortem diagnosis was acute venous infarction of the small and large intestine. To our knowledge, this report is the first to describe the diagnosis and long-term management of polycythemia vera in a ferret and the use of hydroxyurea for this purpose.

  19. Generation of Monoclonal Antibodies against Immunoglobulin Proteins of the Domestic Ferret (Mustela putorius furo)

    Science.gov (United States)

    2017-01-01

    The domestic ferret (Mustela putorius furo) serves as an animal model for the study of several viruses that cause human disease, most notably influenza. Despite the importance of this animal model, characterization of the immune response by flow cytometry (FCM) is severely hampered due to the limited number of commercially available reagents. To begin to address this unmet need and to facilitate more in-depth study of ferret B cells including the identification of antibody-secreting cells, eight unique murine monoclonal antibodies (mAb) with specificity for ferret immunoglobulin (Ig) were generated using conventional B cell hybridoma technology. These mAb were screened for reactivity against ferret peripheral blood mononuclear cells by FCM and demonstrate specificity for CD79β+ B cells. Several of these mAb are specific for the light chain of surface B cell receptor (BCR) and enable segregation of kappa and lambda B cells. Additionally, a mAb that yielded surface staining of nearly all surface BCR positive cells (i.e., pan ferret Ig) was generated. Collectively, these MαF-Ig mAb offer advancement compared to the existing portfolio of polyclonal anti-ferret Ig detection reagents and should be applicable to a wide array of immunologic assays including the identification of antibody-secreting cells by FCM. PMID:28286781

  20. Overview of developmental, reproductive, and behavioral/ neurological effects of mercury exposures in wildlife

    Science.gov (United States)

    Heinz, G.H.; Hoffman, D.; Klimstra, J.; Stebbins, K.

    2007-01-01

    We review wildlife/mercury literature and our own research findings that demonstrate the relevance of wildlife toxicity data in protecting human health. Methylmercury affects wildlife through reduced adult survival and reproduction, aberrant behavior, immune system effects, and teratogenic effects. Methylmercury can readily cross the blood-brain barrier, is excreted into eggs in birds, and is transferred to young mammals across the placenta and in milk. Its principal effect on wildlife is on neurological functions. Wild mink (Mustela vison) and otter (Lutra canadensis) have died from methylmercury poisoning, with signs of poisoning including anorexia, loss of weight, incoordination, tremors, and convulsions, which are symptoms similar to those experienced by mercury-poisoned humans. Mammals also may experience tonic and clonic convulsions and an increase in fetal anomalies, again paralleling toxic problems in people. Antibody-producing cells can be suppressed by methylmercury. Microscopically, the most notable lesions are in the cerebrum. Extensive vacuolation of hepatocytes in the liver and necrosis and other changes in the appearance of the proximal convoluted tubules of the kidneys are often noted. When harp seals (Pagophilus groenlandicus) were dosed with methylmercury chloride the number of circulating erythrocytes decreased and white blood cell counts greatly increased. The poisoned seals also suffered from uremia, hyperproteinemia, hypercholesterolemia, hyperbilirubinemia, and elevations in lactic dehydrogenase and alkaline phosphatase. In birds, signs of methylmercury poisoning included emaciation and weakness in the extremities, which progressed until the birds died. Mercury poisoning in birds and mammals can be diagnosed from a combination of the signs of poisoning if the animal is still alive, the pathological effects seen in a gross necropsy, the histopathological effects seen with a microscope, and the concentrations of mercury in various tissues. Our

  1. Recombinant F1-V fusion protein protects black-footed ferrets (Mustela nigripes) against virulent Yersinia pestis infection

    Science.gov (United States)

    Rocke, Tonie E.; Mencher, J.; Smith, Susan; Friedlander, A.M.; Andrews, G.P.; Baeten, L.A.

    2004-01-01

    Black-footed ferrets (Mustela nigripes) are highly susceptible to sylvatic plague, caused by the bacterium Yersinia pestis, and this disease has severely hampered efforts to restore ferrets to their historic range. A study was conducted to assess the efficacy of vaccination of black-footed ferrets against plague using a recombinant protein vaccine, designated F1-V, developed by personnel at the U.S. Army Medical Research Institute of Infectious Diseases. Seven postreproductive black-footed ferrets were immunized with the vaccine, followed by two booster immunizations on days 23 and 154; three control black-footed ferrets received a placebo. After the second immunization, antibody titers to both F1 and V antigen were found to be significantly higher in vaccinates than controls. On challenge with 7,800 colony-forming units of virulent plague by s.c. injection, the three control animals died within 3 days, but six of seven vaccinates survived with no ill effects. The seventh vaccinate died on day 8. These results indicate that black-footed ferrets can be immunized against plague induced by the s.c. route, similar to fleabite injection.

  2. Developmental changes in the skull morphology of common minke whales Balaenoptera acutorostrata.

    Science.gov (United States)

    Nakamura, Gen; Kato, Hidehiro

    2014-10-01

    We investigated growth-related and sex-related morphological changes in the skulls of 144 North Pacific common minke whales Balaenoptera acutorostrata. Measurement was conducted at 39 points on the skull and mandible to extract individual allometric equations relating the length and zygomatic width of the skull. The results revealed no significant differences in skull morphology by sex except for width of occipital bone. The size relative to the skull of the anatomical parts involved in feeding, such as the rostrum and mandible, increased after birth. In contrast, the sensory organs and the anatomical regions involved in neurological function, such as the orbit, tympanic bullae, and foramen magnum, were fully developed at birth, and their relative size reduced over the course of development. This is the first study to investigate developmental changes in the skull morphology using more than 100 baleen whale specimens, and we believe the results of this study will contribute greatly to multiple areas of baleen whale research, including taxonomy and paleontology. © 2014 Wiley Periodicals, Inc.

  3. The use of operant technology to measure behavioral priorities in captive animals.

    Science.gov (United States)

    Cooper, J J; Mason, G J

    2001-08-01

    Addressing the behavioral priorities of captive animals and the development of practical, objective measures of the value of environmental resources is a principal objective of animal welfare science. In theory, consumer demand approaches derived from human microeconomics should provide valid measures of the value of environmental resources. In practice, however, a number of empirical and theoretical problems have rendered these measures difficult to interpret in studies with animals. A common approach has been to impose a cost on access to resources and to use time with each resource as a measure of consumption to construct demand curves. This can be recorded easily by automatic means, but in a number of studies, it has been found that animals compensate for increased cost of access with longer visit time. Furthermore, direct observation of the test animals' behavior has shown that resource interaction is more intense once the animals have overcome higher costs. As a consequence, measures based on time with the resource may underestimate resource consumption at higher access costs, and demand curves derived from these measures may not be a true reflection of the value of different resources. An alternative approach to demand curves is reservation price, which is the maximum price individual animals are prepared to pay to gain access to resources. In studies using this approach, farmed mink (Mustela vison) paid higher prices for food and swimming water than for resources such as tunnels, water bowls, pet toys, and empty compartments. This indicates that the mink placed a higher value on food and swimming water than on other resources.

  4. Molecular diagnostics of aleutian mink disease virus: applied use of next generation sequencing and phylogenetics

    DEFF Research Database (Denmark)

    Hagberg, Emma Elisabeth

    based either on partial or entire genes, or on pure epidemiological data. Thus, when initiating this project, little was known about AMDV’s total genomic diversity and how the virus was spread between farms. Recent advances in the field of molecular diagnostics have made high throughput tools...... could contribute to the elucidation of AMDV transmission between farms and improve molecular diagnostics. During the first phase of this project a method for performing whole genome sequencing of AMDV was developed. This protocol enabled the sequencing of a large number of in vivo infectious AMDV......-estimates. Altogether, the work presented in this thesis provides a contribution to the molecular diagnostics of AMDV, enables us better to understand the virus’ evolutionary behaviour in the context of mink farming, and is anticipated to be of value for more accurately tracing back in time the emergence of future...

  5. Friend and Moloney murine leukemia viruses specifically recombine with different endogenous retroviral sequences to generate mink cell focus-forming viruses.

    Science.gov (United States)

    Evans, L H; Cloyd, M W

    1985-01-01

    A group of mink cell focus-forming (MCF) viruses was derived by inoculation of NFS/N mice with Moloney murine leukemia virus (Mo-MuLV 1387) and was compared to a similarly derived group of MCF viruses from mice inoculated with Friend MuLV (Fr-MuLV 57). Antigenic analyses using monoclonal antibodies specific for MCF virus and xenotropic MuLV envelope proteins and genomic structural analyses by RNase T1-resistant oligonucleotide finger-printing indicated that the Moloney and Friend MCF viruses arose by recombination of the respective ecotropic MuLVs with different endogenous retrovirus sequences of NFS mice.

  6. Separating the mink dam from the litter at 7 or 8 weeks after delivery

    DEFF Research Database (Denmark)

    Malmkvist, Jens; Palme, Rupert; Larsen, Torben

    2015-01-01

    The optimal timing of separating the mink dam from the litter is suggested to be a balance between the partly conflicting needs of the mother and the kits. Early removal of the dam or partial removal of the litter may protect the dam against exhaustion. Little is known about the maternal motivation...... around the time of separation. Therefore, we investigated the effects of separating the dam from the litter, using brown first-parity dams (N=374) randomly assigned within each date of delivery to two treatment groups: The dam was taken away from the litter either at day 49 ±1 (7w, N=185) or at day 56 ±1...... (8w, N=189) after birth. The aim was to investigate whether the dams had a different motivation to take care of the litter after 7 and 8 weeks, estimated by non-invasive determination of cortisol (FCM: Faecal Corticsol Metabolites) and dam calls the first week after separation. The two treatment...

  7. A study on the improvement of age estimation in common minke whales using the method of gelatinized extraction of earplug

    Directory of Open Access Journals (Sweden)

    Hikari Maeda

    2013-08-01

    Full Text Available We attempted to settle the potential problems of bias caused by too soft earplugs and poor formation of the growth layers in age readings of common minke whales. Thus, we examined the feasibility of a new technique of incorporating gelatin in order to collect earplugs for age assessment. Frozen sectioning and histology of the earplug core were also used as methods to improve age estimation. Earplugs were collected by filling the space in the external auditory meatus with gelatin, hardening the gelatin, earplug and its fragments, by spraying with cooling gas, and removing the earplug embedded in gelatin. In 174 trials with common minke whales in the Western North Pacific of coastal waters of Japan in 2007–2009, it was revealed that embedding earplugs with gelatin minimized breakage and protected the neonatal line (NL. This method was particularly effective in younger animals. As a result, the readability was improved. We also examined the histological sections, which were sliced using the Kawamoto specialized frozen sectioning technique, and stained them separately with toluidine blue, haematoxylin and eosin, Sudan III, Sudan VII, and alizarin red S to display a clearer core surface image of the growth layers. The histological sections stained with alizarin red S provided the clearest images, in which we could easily identify both dark and pale laminations. This suggested a close relationship with the seasonal changes in calcium intake from feeding. Earlier age estimation methods focused on fat content in the growth layers; however, we found potential for an improvement in the readability of unclear growth layers when focusing on calcium.

  8. Risk factors associated with diarrhea in Danish commercial mink (Neovison vison) during the pre-weaning period

    DEFF Research Database (Denmark)

    Birch, Julie Melsted; Agger, Jens Frederik; Dahlin, Christina

    2017-01-01

    gestation period, and dogs accessing the farm area were significantly associated with being a case farm. Case farms were prescribed almost twice the amount of antibacterials per gestational unit (female and litter) as in control farms. Farmers on case farms spent significantly more time nursing and treating...... the animals and experienced more females with mastitis compared to farmers on control farms. No significant differences in cleaning practices or hygienic measures between case and control farms were found and there were no differences in drinking water quality, bedding material, composition neither of color...... types nor in management regarding litter equalization. Results from this study showed an association between the occurrence of pre-weaning diarrhea on mink farms and parity profile, farm size and feeding intensity in the gestational period. The access of dogs to the farm area was a significant risk...

  9. Energetics of swimming by the ferret: consequences of forelimb paddling.

    Science.gov (United States)

    Fish, Frank E; Baudinette, Russell V

    2008-06-01

    The domestic ferret (Mustela putorius furo) swims by alternate strokes of the forelimbs. This pectoral paddling is rare among semi-aquatic mammals. The energetic implications of swimming by pectoral paddling were examined by kinematic analysis and measurement of oxygen consumption. Ferrets maintained a constant stroke frequency, but increased swimming speed by increasing stroke amplitude. The ratio of swimming velocity to foot stroke velocity was low, indicating a low propulsive efficiency. Metabolic rate increased linearly with increasing speed. The cost of transport decreased with increasing swimming speed to a minimum of 3.59+/-0.28 J N(-1) m(-1) at U=0.44 m s(-1). The minimum cost of transport for the ferret was greater than values for semi-aquatic mammals using hind limb paddling, but lower than the minimum cost of transport for the closely related quadrupedally paddling mink. Differences in energetic performance may be due to the amount of muscle recruited for propulsion and the interrelationship hydrodynamic drag and interference between flow over the body surface and flow induced by propulsive appendages.

  10. Wild Maid, Wild Soul, A Wild Wild Weed: Niki de Saint Phalle’s Fierce Femininities, c. 1960-66

    Directory of Open Access Journals (Sweden)

    Amelia Jones

    2015-11-01

    Full Text Available Self-described as “wild maid,” “wild soul,” “a wild wild weed,” Niki de Saint Phalle narrated herself as artistic subject in a concerted way that stands out in the history of art: as both creatively driven and emotionally renegade and excessive, as both definitively woman and definitively artist. In this essay I take this special case of self-narration, and the particular power of St. Phalle’s work, as an opportunity to explore the relationship between.(auto-biography and artistic practice. The case of St. Phalle, a radical sculptor, performance artist, writer, and filmmaker, allows us to understand the exaggerated way in which women artists were until very recently forced to adopt “fierce femininities” to make a place for themselves as artists. In this way, I suggest that St. Phalle represents a key inspirational force opening the door for second wave feminism and the feminist art movement.

  11. Compensative-rehabilitative responses of blood-forming tissue cells after chronic irradiation.; Kompensatorno-vosstanovitel`nye reaktsii kletok krovetvornoj tkani pri khronicheskom obluchenii.

    Energy Technology Data Exchange (ETDEWEB)

    Nosova, L I; Ryasenko, V I [Yinstitut Zoologyiyi, Natsyional` na Akademyiya Nauk Ukrayini, Kyiv (Ukraine); [Nauchno-Proizvodstvennoe Ob` ` edinenie Pripyat` , Chernobyl (Ukraine)

    1994-12-31

    The bone marrow eosinophils of minks and wild rats subjected to chronical irradiation are able of secreting a peroxidase system obtained by neutrophils. As a result heterophilic granulocytes appear in the peripheral blood. Intercellular transgranulation, emperiopolesis into megakaryocytes and eosinophils as peroxidase donors for neutrophils are regarded as cellular and subcellular adaptations in the mammalian bone marrow after irradiation.

  12. Roles of three amino acids of capsid proteins in mink enteritis parvovirus replication.

    Science.gov (United States)

    Mao, Yaping; Su, Jun; Wang, Jigui; Zhang, Xiaomei; Hou, Qiang; Bian, Dawei; Liu, Weiquan

    2016-08-15

    Virulent mink enteritis parvovirus (MEV) strain MEV-LHV replicated to higher titers in feline F81 cells than attenuated strain MEV-L. Phylogenetic and sequence analyses of the VP2 gene of MEV-LHV, MEV-L and other strains in GenBank revealed two evolutionary branches separating virulent and attenuated strains. Three residues, 101, 232 and 411, differed between virulent and attenuated strains but were conserved within the two branches. Site-directed mutagenesis of the VP2 gene of infectious plasmids of attenuated strain MEV-L respectively replacing residues 101 Ile and 411 Ala with Thr and Glu of virulent strains (MEV-L I101T and MEV-L A411E) increased replication efficiency but still to lower levels than MEV-LHV. However, viruses with mutation of residue 232 (MEV-L I232V and MEV-L I101T/I232V/A411E) decreased viral transcription and replication levels. The three VP2 residues 101, 232 and 411, located on or near the capsid surface, played different roles in the infection processes of MEV. Copyright © 2016 Elsevier B.V. All rights reserved.

  13. Screening for antibodies against Aleutian disease virus (ADV) in mink. Elucidation of dubious results by additive counterimmunoelectrophoresis

    DEFF Research Database (Denmark)

    Uttenthal, Åse

    1992-01-01

    In order to distinguish true positive results in counterimmunoelectrophoresis from false positive ones an additive counterimmunoelectrophoresis was developed. The method was tested on selected mink serum samples as part of a routine testing for antibodies towards Aleutian disease virus on 3 million...... blood samples. The procedure of the method is, that a known positive serum sample is mixed with the patient serum to be tested. The result from a false positive sample will be one precipitin line towards virus and one nonspecific line. If the serum sample is a true positive one, the antibodies...... originating from the patient serum will be added to the antibodies in the standard positive serum giving only one precipitin line. The system is further extended by testing the serum samples towards an antigen preparation containing all the cellular components but free from virus....

  14. Weaning and separation stress: maternal motivation decreases with litter age and litter size in farmed mink

    DEFF Research Database (Denmark)

    Malmkvist, Jens; Sørensen, Dennis Dam; Larsen, Torben

    2016-01-01

    and maternal motivation around the time of weaning and separation. Therefore, we investigated effects of separating the dam from the litter using brown first-parity farm mink dams (n = 374) taken away from the litter either day 49 ± 1 (7w, n = 185) or day 56 ± 1 (8w, n = 189) after birth. The aim...... was to investigate whether the dams experienced stress/had a different motivation to be reunited with the litter after7 and 8 weeks, estimated by non-invasive determination of cortisol (FCM: Faecal Cortisol Metabolites)and dam behaviour including calls the first week after separation (D0: Day of removal, D1: next.......024). We interpret these results as a higher maternal motivation in dams at 7 weeks than at 8 weeks after birth. Additionally, the separation-induced calling in dams decreased with increasing litter size (P = 0.022). Thus in addition to litter age, the size of the litter is important for the maternal...

  15. Spatiotemporal trends in Canadian domestic wild boar production and habitat predict wild pig distribution

    DEFF Research Database (Denmark)

    Michel, Nicole; Laforge, Michel; van Beest, Floris

    2017-01-01

    eradication of wild pigs is rarely feasible after establishment over large areas, effective management will depend on strengthening regulations and enforcement of containment practices for Canadian domestic wild boar farms. Initiation of coordinated provincial and federal efforts to implement population...... wild boar and test the propagule pressure hypothesis to improve predictive ability of an existing habitat-based model of wild pigs. We reviewed spatiotemporal patterns in domestic wild boar production across ten Canadian provinces during 1991–2011 and evaluated the ability of wild boar farm...... distribution to improve predictive models of wild pig occurrence using a resource selection probability function for wild pigs in Saskatchewan. Domestic wild boar production in Canada increased from 1991 to 2001 followed by sharp declines in all provinces. The distribution of domestic wild boar farms in 2006...

  16. Evaluation of the immune response after vaccination against distemper at a mink (Mustela vison) farm in Argentina

    OpenAIRE

    Jar, A. M.; Ramayo, L. G.; Stempler, A.; Goldman, L. H.; Mundo, S. L.

    2010-01-01

    El virus del distemper produce una enfermedad en los visones que cursa con síntomas respiratorios, gastrointestinales, neurológicos y cutáneos y presenta alta morbilidad y mortalidad, principalmente en los cachorros. La enfermedad se controla mediante la aplicación de vacunas destinadas a esta especie. El objetivo del trabajo fue determinar el título de anticuerpos neutralizantes contra el virus del distemper en visones vacunados y no vacunados de un criadero de Argentina. Se analizó la cinét...

  17. FLEAS OF BLACK-FOOTED FERRETS (MUSTELA NIGRIPES) AND THEIR POTENTIAL ROLE IN THE MOVEMENT OF PLAGUE.

    Science.gov (United States)

    Mize, Erica L; Grassel, Shaun M; Britten, Hugh B

    2017-07-01

    Sylvatic plague is one of the major impediments to the recovery of the black-footed ferret ( Mustela nigripes ) because it decimates their primary prey species, prairie dogs ( Cynomys spp.), and directly causes mortality in ferrets. Fleas are the primary vector of Yersinia pestis , the causative agent of sylvatic plague. The goal of this research was to better understand the flea fauna of ferrets and the factors that might influence flea abundance on ferrets. Fleas from ferrets were tested for Y. pestis in a post hoc assessment to investigate the plausibility that some ferrets could act as incidental transporter hosts of fleas infected with Y. pestis . Fleas were collected from ferrets captured on the Lower Brule Indian Reservation in central South Dakota, US from 2009 to 2012. A total of 528 fleas collected from 67 individual ferrets were identified and tested for the presence of Y. pestis with a nested PCR assay. The predominant flea recovered from ferrets was Oropsylla hirsuta , a species that comprises 70-100% of the fleas recovered from prairie dogs and their burrows in the study area. Yersinia pestis was detected at low levels in fleas collected from ferrets with prevalence ranging from 0% to 2.9%; male ferrets harbored significantly more fleas than female ferrets. Six of 67 ferrets vaccinated against plague carried fleas that tested positive for Y. pestis , which suggests ferrets vaccinated against plague could inadvertently act as incidental transporter hosts of Y. pestis -positive fleas.

  18. Ticks (Acari: Ixodida) on wild carnivores in Brazil.

    Science.gov (United States)

    Labruna, Marcelo B; Jorge, Rodrigo S P; Sana, Dênis A; Jácomo, Anah Tereza A; Kashivakura, Cyntia K; Furtado, Mariana M; Ferro, Claudia; Perez, Samuel A; Silveira, Leandro; Santos, Tarcísio S; Marques, Samuel R; Morato, Ronaldo G; Nava, Alessandra; Adania, Cristina H; Teixeira, Rodrigo H F; Gomes, Albério A B; Conforti, Valéria A; Azevedo, Fernando C C; Prada, Cristiana S; Silva, Jean C R; Batista, Adriana F; Marvulo, Maria Fernanda V; Morato, Rose L G; Alho, Cleber J R; Pinter, Adriano; Ferreira, Patrícia M; Ferreira, Fernado; Barros-Battesti, Darci M

    2005-01-01

    The present study reports field data of ticks infesting wild carnivores captured from July 1998 to September 2004 in Brazil. Additional data were obtained from one tick collection and from previous published data of ticks on carnivores in Brazil. During field work, a total of 3437 ticks were collected from 89 Cerdocyon thous (crab-eating fox), 58 Chrysocyon brachyurus (maned wolf), 30 Puma concolor (puma), 26 Panthera onca (jaguar), 12 Procyon cancrivorus (crab-eating raccoon), 4 Speothos venaticus (bush dog), 6 Pseudalopex vetulus (hoary fox), 6 Nasua nasua (coati), 6 Leopardus pardalis (ocelot), 2 Leopardus tigrinus (oncilla), 1 Leopardus wiedii (margay), 1 Herpailurus yagouaroundi (jaguarundi), 1 Oncifelis colocolo (pampas cat), 1 Eira barbara (tayara), 1 Galictis vittata (grison), 1 Lontra longicaudis (neotropical otter), and 1 Potus flavus (kinkajou). Data obtained from the Acari Collection IBSP included a total of 381 tick specimens collected on 13 C. thous, 8 C. brachyurus, 3 P. concolor, 10 P. onca, 3 P. cancrivorus, 4 N. nasua, 1 L. pardalis, 1 L. wiedii, 4 H. yagouaroundi, 1 Galictis cuja (lesser grison), and 1 L. longicaudis. The only tick-infested carnivore species previously reported in Brazil, for which we do not present any field data are Pseudalopex gymnocercus (pampas fox), Conepatus chinga (Molina's hog-nosed skunk), and Conepatus semistriatus (striped hog-nosed skunk). We report the first tick records in Brazil on two Felidae species (O. colocolo, H. yagouaroundi), two Canidae species (P. vetulus, S. venaticus), one Procyonidae species (P. flavus) and one Mustelidae (E. barbara). Tick infestation remains unreported for 5 of the 26 Carnivora species native in Brazil: Oncifelis geoffroyi (Geoffroy's cat), Atelocynus microtis (short-eared dog), Pteronura brasiliensis (giant otter), Mustela africana (Amazon weasel), and Bassaricyon gabbii (olingo). Our field data comprise 16 tick species represented by the genera Amblyomma (12 species), Ixodes (1

  19. Yearling mink dams fed restricted in early lactation have less mammary gland tissue six weeks after birth

    DEFF Research Database (Denmark)

    Møller, Steen Henrik; Pinkalski, Mariann Nakano

    2015-01-01

    The optimal timing of separating the mink dam from the litter is suggested to be a balance between the partly conflicting needs of the mother and the kits. Early removal of the dam or partial removal of the litter may protect the dam against exhaustion. Little is known about the maternal motivation...... around the time of separation. Therefore, we investigated the effects of separating the dam from the litter, using brown first-parity dams (N=374) randomly assigned within each date of delivery to two treatment groups: The dam was taken away from the litter either at day 49 ±1 (7w, N=185) or at day 56 ±1...... (8w, N=189) after birth. The aim was to investigate whether the dams had a different motivation to take care of the litter after 7 and 8 weeks, estimated by non-invasive determination of cortisol (FCM: Faecal Corticsol Metabolites) and dam calls the first week after separation. The two treatment...

  20. Early transfer of mated females into the maternity unit reduces stress and increases maternal care in farm mink

    DEFF Research Database (Denmark)

    Malmkvist, Jens; Palme, Rupert

    2015-01-01

    Mated mammals on farms are typically transferred to another housing environment prior to delivery. We investigated whether the timing of this transfer – EARLY (Day −36), INTERMEDIATE (Day −18), or LATE (Day −3) relative to the expected day of birth (Day 0) – affects maternal stress, maternal care...... sized groups (n = 60): (i) ‘EARLY’, transfer to maternity unit immediately after the end of the mating period, March 23; (ii) ‘INTERMEDIATE’, transfer in the middle of the period, April 10; (iii) ‘LATE’, transfer late in the pregnancy period, April 25. Data collection included weekly determination...... of faecal cortisol metabolites (FCM) and evaluation of maternal care: nest building, in-nest temperature, plus kit-retrieval behaviour, kit mortality and growth day 0–7 postpartum. We document that mated mink females build and maintain a nest at least 1 month prior to delivery when transferred...

  1. Wild harvest

    NARCIS (Netherlands)

    Cruz-Garcia, G.S.; Struik, P.C.; Johnson, D.E.

    2016-01-01

    Rice fields provide not only a staple food but are also bio-diverse and multi-functional ecosystems. Wild food plants are important elements of biodiversity in rice fields and are critical components to the subsistence of poor farmers. The spatial and seasonal distribution of wild food plants

  2. Protein and amino acid bioavailability of extruded dog food with protein meals of different quality using growing mink (Neovison vison) as a model

    DEFF Research Database (Denmark)

    Tjernsbekk, M. T.; Tauson, Anne-Helene; Matthiesen, Connie Frank

    2016-01-01

    with respect to CP digestibility and AA composition and included lamb meal (LBM), poultry meal (PM), and fish meal (FM) with low, intermediate, and high protein quality, respectively. Nitrogen balance, BW gain, protein efficiency ratio (PER), and apparent total tract digestibility (ATTD) were used as measures...... by the European Pet Food Industry Federation. It was concluded that growth studies with mink kits can provide valuable information in protein quality assessment of extruded dog foods. Furthermore, the study showed that to ensure nutritional adequacy of dog food and to be able to compare protein quality of dog...

  3. Dietary values of wild and semi-wild edible plants in Southern Ethiopia

    African Journals Online (AJOL)

    However, traditional processing methods lower most of the anti-nutritionals and their respective risks. New food composition tables that integrate indigenous knowledge and nutritional content of the semi-wild and wild edibles are recommended. Wild edibles can be considered to improve livelihood security and reduce ...

  4. Wild dogma II: The role and implications of wild dogma for wild dog management in Australia

    Directory of Open Access Journals (Sweden)

    Benjamin L. ALLEN, Richard M. ENGEMAN, Lee R. ALLEN

    2011-12-01

    Full Text Available The studies of Allen (2011 and Allen et al. (2011 recently examined the methodology underpinning claims that dingoes provide net benefits to biodiversity by suppressing foxes and cats. They found most studies to have design flaws and/or observational methods that preclude valid interpretations from the data, describing most of the current literature as ‘wild dogma’. In this short supplement, we briefly highlight the roles and implications of wild dogma for wild dog management in Australia. We discuss nomenclature, and the influence that unreliable science can have on policy and practice changes related to apex predator management [Current Zoology 57 (6: 737–740, 2011].

  5. [The growth of attenuated strains of canine parvovirus, mink enteritis virus, feline panleukopenia virus, and rabies virus on various types of cell cultures].

    Science.gov (United States)

    Zuffa, T

    1987-10-01

    The growth characteristics were studied in the attenuated strains of canine parvovirus CPVA-BN 80/82, mink enteritis virus MEVA-BN 63/82 and feline panleucopenia virus FPVA-BN 110/83 on the stable feline kidney cell line FE, and in the attenuated canine distemper virus CDV-F-BN 10/83 on chicken embryo cell cultures (KEB) and cultures of the stable cell line VERO. When the FE cultures were infected with different parvoviruses in cell suspension at MOI 2-4 TKID50 per cell, the first multiplication of the intracellular virus was recorded 20 hours p. i. In the canine parvovirus, the content of intracellular and extracellular virus continued increasing parallelly until the fourth day; then, from the fourth to the sixth day, the content of extracellular virus still increased whereas that of intracellular virus fell rapidly. In the case of the mink enteritis virus the release of the virus into the culture medium continued parallelly with the production of the cellular virus until the sixth day. In the case of the feline panleucopenia virus the values concerning free virus and virus bound to cells were lower, starting from the second day p. i. When KEB or VERO cultures were infected in cell suspension with the canine distemper virus at MOI about 0.004 per 1 cell, the replicated intracellular virus was first recorded in the KEB cultures five hours after infection but in the VERO cultures only 20 hours after infection, with a timely release of the virus into the culture medium in both kinds of tissue. In the KEB and VERO cultures the highest values of infection titres were recorded on the fourth day p. i., the course of virus multiplication on the cells being parallel with its release into the culture medium.

  6. Use of bovine recombinant prion protein and real-time quaking-induced conversion to detect cattle transmissible mink encephalopathy prions and discriminate classical and atypical L- and H-Type bovine spongiform encephalopathy.

    Science.gov (United States)

    Hwang, Soyoun; Greenlee, Justin J; Nicholson, Eric M

    2017-01-01

    Prions are amyloid-forming proteins that cause transmissible spongiform encephalopathies through a process involving conversion from the normal cellular prion protein to the pathogenic misfolded conformation (PrPSc). This conversion has been used for in vitro assays including serial protein misfolding amplification and real-time quaking induced conversion (RT-QuIC). RT-QuIC can be used for the detection of prions in a variety of biological tissues from humans and animals. Extensive work has been done to demonstrate that RT-QuIC is a rapid, specific, and highly sensitive prion detection assay. RT-QuIC uses recombinant prion protein to detect minute amounts of PrPSc. RT-QuIC has been successfully used to detect PrPSc from different prion diseases with a variety of substrates including hamster, human, sheep, bank vole, bovine and chimeric forms of prion protein. However, recombinant bovine prion protein has not been used to detect transmissible mink encephalopathy (TME) or to differentiate types of bovine spongiform encephalopathy (BSE) in samples from cattle. We evaluated whether PrPSc from TME and BSE infected cattle can be detected with RT-QuIC using recombinant bovine prion proteins, and optimized the reaction conditions to specifically detect cattle TME and to discriminate between classical and atypical BSE by conversion efficiency. We also found that substrate composed of the disease associated E211K mutant protein can be effective for the detection of TME in cattle and that wild type prion protein appears to be a practical substrate to discriminate between the different types of BSEs.

  7. Wild reindeer of Yakutia

    Directory of Open Access Journals (Sweden)

    V.M. Safronov

    1996-01-01

    Full Text Available Three major herds of wild reindeer (Rangifer tarandus tarandus L., totaling over 200,000 animals, occur in the tundra and taiga of northern Yakutia. These herds have been expanding since the late 1950s and now occupy most of their historic range. In addition, several thousand wild reindeer occupy the New Siberian Islands and adjacent coastal mainland tundra, and there are about 60,000 largely sedentary forest reindeer in mountainous areas of the southern two-thirds of the province. Wild reindeer are commercially hunted throughout the mainland, and the production of wild meat is an important part of the economy of the province and of individual reindeer enterprises which produce both wild and domestic meat.

  8. Wild genius - domestic fool? Spatial learning abilities of wild and domestic guinea pigs

    Directory of Open Access Journals (Sweden)

    Sachser Norbert

    2010-03-01

    Full Text Available Abstract Background Domestic animals and their wild relatives differ in a wide variety of aspects. The process of domestication of the domestic guinea pig (Cavia aperea f. porcellus, starting at least 4500 years ago, led to changes in the anatomy, physiology, and behaviour compared with their wild relative, the wild cavy, Cavia aperea. Although domestic guinea pigs are widely used as a laboratory animal, learning and memory capabilities are often disregarded as being very scarce. Even less is known about learning and memory of wild cavies. In this regard, one striking domestic trait is a reduction in relative brain size, which in the domesticated form of the guinea pig amounts to 13%. However, the common belief, that such a reduction of brain size in the course of domestication of different species is accomplished by less learning capabilities is not at all very well established in the literature. Indeed, domestic animals might also even outperform their wild conspecifics taking advantage of their adaptation to a man-made environment. In our study we compared the spatial learning abilities of wild and domestic guinea pigs. We expected that the two forms are different regarding their learning performance possibly related to the process of domestication. Therefore wild cavies as well as domestic guinea pigs of both sexes, aged 35 to 45 days, were tested in the Morris water maze to investigate their ability of spatial learning. Results Both, wild cavies and domestic guinea pigs were able to learn the task, proving the water maze to be a suitable test also for wild cavies. Regarding the speed of learning, male as well as female domestic guinea pigs outperformed their wild conspecifics significantly. Interestingly, only domestic guinea pigs showed a significant spatial association of the platform position, while other effective search strategies were used by wild cavies. Conclusion The results demonstrate that domestic guinea pigs do not at all

  9. Wild genius - domestic fool? Spatial learning abilities of wild and domestic guinea pigs.

    Science.gov (United States)

    Lewejohann, Lars; Pickel, Thorsten; Sachser, Norbert; Kaiser, Sylvia

    2010-03-25

    Domestic animals and their wild relatives differ in a wide variety of aspects. The process of domestication of the domestic guinea pig (Cavia aperea f. porcellus), starting at least 4500 years ago, led to changes in the anatomy, physiology, and behaviour compared with their wild relative, the wild cavy, Cavia aperea. Although domestic guinea pigs are widely used as a laboratory animal, learning and memory capabilities are often disregarded as being very scarce. Even less is known about learning and memory of wild cavies. In this regard, one striking domestic trait is a reduction in relative brain size, which in the domesticated form of the guinea pig amounts to 13%. However, the common belief, that such a reduction of brain size in the course of domestication of different species is accomplished by less learning capabilities is not at all very well established in the literature. Indeed, domestic animals might also even outperform their wild conspecifics taking advantage of their adaptation to a man-made environment.In our study we compared the spatial learning abilities of wild and domestic guinea pigs. We expected that the two forms are different regarding their learning performance possibly related to the process of domestication. Therefore wild cavies as well as domestic guinea pigs of both sexes, aged 35 to 45 days, were tested in the Morris water maze to investigate their ability of spatial learning. Both, wild cavies and domestic guinea pigs were able to learn the task, proving the water maze to be a suitable test also for wild cavies. Regarding the speed of learning, male as well as female domestic guinea pigs outperformed their wild conspecifics significantly. Interestingly, only domestic guinea pigs showed a significant spatial association of the platform position, while other effective search strategies were used by wild cavies. The results demonstrate that domestic guinea pigs do not at all perform worse than their wild relatives in tests of spatial

  10. The gastrointestinal tract of farmed mink (Neovison vison ) maintains a diverse mucosa-associated microbiota following a 3-day fasting period

    DEFF Research Database (Denmark)

    Bahl, Martin Iain; Hammer, Anne S.; Clausen, Tove

    2017-01-01

    Although it is well documented that the gut microbiota plays an important role in health and disease in mammalian species, this area has been poorly studied among carnivorous animals, especially within the mustelidae family. The gastrointestinal tract of carnivores is characterized by its short...... length and fast transit time, as compared to omnivores and herbivores, which is due to the low level of inherent fermentation. Mink represents an example of this, which have a GI tract only four times the length of the body and a transit time of approximately 4–5 hr. In this study, we used high...... interindividual differences in bacterial composition between individual animals being dominated generally by the phylum Firmicutes, but in some cases also Proteobacteria or Fusobacteria. The bacterial load and community structure within the mucus was not severely impacted by 3 days of fasting, which implies...

  11. Is a wild mammal kept and reared in captivity still a wild animal?

    Science.gov (United States)

    Künzl, Christine; Kaiser, Sylvia; Meier, Edda; Sachser, Norbert

    2003-01-01

    This study compared domestic guinea pigs (Cavia aperea f. porcellus; DGP) and two different populations of the wild cavy (Cavia aperea), its ancestor, to examine whether rearing of wild mammals in captivity affects their behavior and physiological stress responses. One population of wild cavies consisted of wild-trapped animals and their first laboratory-reared offspring (WGP-1). The animals of the other population were reared in captivity for about 30 generations (WGP-30). The spontaneous behavior of each of six groups of WGP-1 and WGP-30 and nine groups of DGP, each consisting of one adult male and two adult females, was analyzed quantitatively. Blood samples of the males were taken to determine cortisol, epinephrine, and norepinephrine concentrations. In addition, the exploratory behavior of 60-day-old male WGP-1, WGP-30, and DGP was investigated in an exploration apparatus. The domesticated animals displayed significantly less aggression, but significantly more sociopositive and male courtship behavior than their wild ancestors. In addition, DGP were much less attentive to their physical environment. Surprisingly, no behavioral difference was found between WGP-1 and WGP-30. Basal cortisol concentrations did not differ between wild and domestic guinea pigs. Catecholamine concentrations, however, as well as the challenge values of cortisol, were distinctly reduced in the DGP. WGP-1 and WGP-30 did not differ with respect to their endocrine stress responses. In the exploration apparatus both forms of wild cavies were much more explorative than the domestic animals. These data suggest that the long-term breeding and rearing of wild guinea pigs in captivity do not result in significant changes in behavior and hormonal stress responses. It appears to take much longer periods of time and artificial selection by humans to bring about characters of domestication in wild animals.

  12. 29 CFR 780.114 - Wild commodities.

    Science.gov (United States)

    2010-07-01

    ... Agricultural Or Horticultural Commodities § 780.114 Wild commodities. Employees engaged in the gathering or harvesting of wild commodities such as mosses, wild rice, burls and laurel plants, the trapping of wild... 29 Labor 3 2010-07-01 2010-07-01 false Wild commodities. 780.114 Section 780.114 Labor Regulations...

  13. Woodlands House Nursing Home, Trim Road, Navan, Meath.

    LENUS (Irish Health Repository)

    O'Toole, Paul W

    2010-03-10

    Abstract Background Helicobacter mustelae causes gastritis, ulcers and gastric cancer in ferrets and other mustelids. H. mustelae remains the only helicobacter other than H. pylori that causes gastric ulceration and cancer in its natural host. To improve understanding of H. mustelae pathogenesis, and the ulcerogenic and carcinogenic potential of helicobacters in general, we sequenced the H. mustelae genome, and identified 425 expressed proteins in the envelope and cytosolic proteome. Results The H. mustelae genome lacks orthologs of major H. pylori virulence factors including CagA, VacA, BabA, SabA and OipA. However, it encodes ten autotransporter surface proteins, seven of which were detected in the expressed proteome, and which, except for the Hsr protein, are of unknown function. There are 26 putative outer membrane proteins in H. mustelae, some of which are most similar to the Hof proteins of H. pylori. Although homologs of putative virulence determinants of H. pylori (NapA, plasminogen adhesin, collagenase) and Campylobacter jejuni (CiaB, Peb4a) are present in the H. mustelae genome, it also includes a distinct complement of virulence-related genes including a haemagglutinin\\/haemolysin protein, and a glycosyl transferase for producing blood group A\\/B on its lipopolysaccharide. The most highly expressed 264 proteins in the cytosolic proteome included many corresponding proteins from H. pylori, but the rank profile in H. mustelae was distinctive. Of 27 genes shown to be essential for H. pylori colonization of the gerbil, all but three had orthologs in H. mustelae, identifying a shared set of core proteins for gastric persistence. Conclusions The determination of the genome sequence and expressed proteome of the ulcerogenic species H mustelae provides a comparative model for H. pylori to investigate bacterial gastric carcinogenesis in mammals, and to suggest ways whereby cag minus H. pylori strains might cause ulceration and cancer. The genome sequence was

  14. Early kit mortality and growth in farmed mink are affected by litter size rather than nest climate.

    Science.gov (United States)

    Schou, T M; Malmkvist, J

    2017-09-01

    We investigated the effects of nest box climate on early mink kit mortality and growth. We hypothesised that litters in warm nest boxes experience less hypothermia-induced mortality and higher growth rates during the 1st week of life. This study included data from 749, 1-year-old breeding dams with access to nesting materials. Kits were weighed on days 1 and 7, dead kits were collected daily from birth until day 7 after birth, and nest climate was measured continuously from days 1 to 6. We tested the influences of the following daily temperature (T) and humidity (H) parameters on the number of live-born kit deaths and kit growth: T mean, T min, T max, T var (fluctuation) and H mean. The nest microclimate experienced by the kits was buffered against the ambient climate, with higher temperatures and reduced climate fluctuation. Most (77.0%) live-born kit deaths in the 1st week occurred on days 0 and 1. Seven of 15 climate parameters on days 1 to 3 had significant effects on live-born kit mortality. However, conflicting effects among days, marginal effects and late effects indicated that climate was not the primary cause of kit mortality. Five of 30 climate parameters had significant effects on kit growth. Few and conflicting effects indicated that the climate effect on growth was negligible. One exception was that large nest temperature fluctuations on day 1 were associated with reduced deaths of live-born kit (P<0.001) and increased kit growth (P=0.003). Litter size affected kit vitality; larger total litter size at birth was associated with greater risks of kit death (P<0.001) and reduced growth (P<0.001). The number of living kits in litters had the opposite effect, as kits in large liveborn litters had a reduced risk of death (P<0.001) and those with large mean litter size on days 1 to 7 had increased growth (P=0.026). Nest box temperature had little effect on early kit survival and growth, which could be due to dams' additional maternal behaviour. Therefore, we

  15. [Surveillance on severe acute respiratory syndrome associated coronavirus in animals at a live animal market of Guangzhou in 2004].

    Science.gov (United States)

    Wang, Ming; Jing, Huai-qi; Xu, Hui-fang; Jiang, Xiu-gao; Kan, Biao; Liu, Qi-yong; Wan, Kang-lin; Cui, Bu-yun; Zheng, Han; Cui, Zhi-gang; Yan, Mei-ying; Liang, Wei-li; Wang, Hong-xia; Qi, Xiao-bao; Li, Zhen-jun; Li, Ma-chao; Chen, Kai; Zhang, En-min; Zhang, Shou-yin; Hai, Rong; Yu, Dong-zheng; Xu, Jian-guo

    2005-02-01

    To study the prevalence of severe acute respiratory syndrome coronavirus (SARS-CoV) like virus in animals at a live animal market of Guanzhou in 2004 before and after culling of wild animal action taken by the local authority, in order to predict the re-emerging of SARS from animal originals in this region. Animals at live animal market were sampled for rectal and throat swabs in triplicate. A single step realtime reverse transcription-polymerase chain reaction (RT-PCR) diagnostic kit was performed for screening SARS-CoV like virus, the manual nested RT- PCR and DNA sequencing were performed for confirmation. Only specimens which tested positive for both of the N and P genes by nested RT-PCR were scored as positive. In 31 animals sampled in January 5 2004 before culling of wild animals at Guangdong Province, including 20 cats (Felis catus), 5 red fox (Vulpes vulpes) and 6 Lesser rice field rats (Rattus losea), 8 (25.8%) animals were tested positive for SARS-CoV like virus by RT-PCR methods, of which 4 cats, 3 red fox and one Lesser rice field rats were included. However, two weeks after culling of animals and disinfection of the market were implemented, in 119 animals sampled in January 20 2004, including 6 rabbits (Oryctolagus cuniculus), 13 cats, 46 red jungle fowl (Gallus gallus), 13 spotbill duck (Anas platyrhynchos), 10 greylag goose (Anser anser), 31 Chinese francolin (Franclinus pintadeanus), only rectal swab from one greylag goose was tested positive for SARS-CoV like virus. Furthermore, in 102 animals that including 14 greylag gooses, 3 cats, 5 rabbits, 9 spotbill duck (Anaspoecilorhyncha), 2 Chinese francolin (Franclinus pintadeanus), 8 common pheasant (Phasianus colchicus), 6 pigeons, 9 Chinese muntjac (Muntiacus reevesi), 19 wild boar (Sus scrofa), 16 Lesser rice field rats, 5 dogs, 1 mink (Mustela vison), 3 goats, 2 green peafowl (Pavo muticus) sampled in April, May, June, July, August and November, only rectal swab from one pig was tested positive

  16. A study on the comparison of antioxidant effects among wild ginseng, cultivated wild ginseng, and cultivated ginseng extracts

    Directory of Open Access Journals (Sweden)

    Hae Young, Jang

    2008-09-01

    Full Text Available Objective : The objective of this study was to compare the antioxidant effects among wild ginseng, cultivated wild ginseng, and ginseng extracts. Methods : In vitro antioxidant activities were examined by total antioxidant capacity (TAC, oxygen radical scavenging capacity(ORAC, total phenolic content, 1, 1-Diphenyl-2-picrylhydrazyl (DPPH radical scavenging activity, inhibition of induced lipid peroxidation using liver mitochondria, reactive oxygen species(ROS scavenging effect using 2’, 7’-dichlorofluorescein(DCF fluorescence. Results : 1. TAC of 1.5 and 3.75 mg extracts was highest in cultivated wild ginseng, followed by wild ginseng and lowest in ginseng. 2. ORAC of 2, 10, and 20 μg extracts was highest in cultivated wild ginseng, followed by wild ginseng and lowest in ginseng. 3. Total phenolic content of 0.375, 0.938, and 1.875 mg extracts was highest in cultivated wild ginseng, followed by wild ginseng and lowest in ginseng. 4. DPPH(1, 1 -Diphenyl-2-picrylhydrazyl scavenging activity between wild ginseng and cultivated wild ginseng did not differ significantly (p>0.05. 5. Induced lipid peroxidation, measured by TBARS concentration in solution containing rat liver mitochondria incubated in the presence of FeSO4/ascorbic acid was inhibited as amounts of wild ginseng, cultivated wild ginseng, and ginseng extracts increased. TBARS concentration of ginseng extracts were significantly (p<0.05 higher than wild ginseng or cultivated wild ginseng extracts. 6. DCF fluorescence intensity was decreased as concentrations of wild ginseng, cultivated wild ginseng, and ginseng extracts increased, demonstrating that ROS generation was inhibited in a concentrationdependent manner. Conclusions : In summary, the results of this study demonstrate that cultivated wild ginseng extracts had similar antioxidant activities to wild ginseng extracts and greater that of cultivated ginseng extracts.

  17. A multilocus evaluation of ermine (Mustela erminea) across the Holarctic, testing hypotheses of Pleistocene diversification in response to climate change

    Science.gov (United States)

    Dawson, Natalie G.; Hope, Andrew G.; Talbot, Sandra L.; Cook, Joseph A.

    2013-01-01

    Aim: We examined data for ermine (Mustela erminea) to test two sets of diversification hypotheses concerning the number and location of late Pleistocene refugia, the timing and mode of diversification, and the evolutionary influence of insularization. Location: Temperate and sub-Arctic Northern Hemisphere. Methods: We used up to two mitochondrial and four nuclear loci from 237 specimens for statistical phylogeographical and demographic analyses. Coalescent species-tree estimation used a Bayesian approach for clade divergence based on external mutation rate calibrations. Approximate Bayesian methods were used to assess population size, timing of divergence and gene flow. Results: Limited structure coupled with evidence of population growth across broad regions, including previously ice-covered areas, indicated expansion from multiple centres of differentiation, but high endemism along the North Pacific coast (NPC). A bifurcating model of diversification with recent growth spanning three glacial cycles best explained the empirical data. Main conclusions: A newly identified clade in North America indicated a fourth refugial area for ermine. The shallow coalescence of all extant ermine reflects a recent history of diversification overlying a deeper fossil record. Post-glacial colonization has led to potential contact zones for multiple lineages in north-western North America. A model of diversification of ermine accompanied by recent gene flow was marginally less well supported than a model of divergence of major clades in response to the most recent glacial cycles.

  18. Component analysis of cultivated ginseng, cultivated wild ginseng, and natural wild ginseng by structural parts using HPLC method

    Directory of Open Access Journals (Sweden)

    Young-Ju,Han

    2007-02-01

    Full Text Available Objectives : The aim of this experiments is to provide an objective differentiation of ginseng, Korean and Chinese cultivated wild ginseng, and natural wild ginseng through components analysis of different parts of ginseng. Methods : Comparative analyses of ginsenoside-, ginsenoside-, and ginsenosides and from the root, stem, and leaves of ginseng, Korean and Chinese cultivated wild ginseng, and natural wild ginseng were conducted using HPLC. Results : 1. For content comparison of leaves, ginseng showed highest content of ginsenoside than other samples. Natural wild ginseng showed relatively high content of ginsenosides and than other samples. 2. For content comparison of the stem, ginseng and 10 years old Chinese cultivated wild ginseng didn't contain ginsenoside . Natural wild ginseng showed higher content of ginsenosides and than other samples. 3. For content comparison of the root, ginsenoside was found only in 5 and 10 years old Korean cultivated wild ginseng. 4. Distribution of contents by the parts of ginseng was similar in ginseng and Chinese cultivated wild ginseng. Conclusions : Above experiment data can be an important indicator for the identification of ginseng, Korean and Chinese cultivated wild ginseng, and natural wild ginseng.

  19. Environmental Assessment for Wild Horse Gathering Inside and Outside Wild Horse Herd Management Areas

    OpenAIRE

    United States Department of the Interior, Bureau of Land Management

    1999-01-01

    Enclosed you will find the Environmental Assessment (EA) which describes the impacts of gathering wild horses in the Rock Springs Field Office area. Gathering wild horses would take place in the Great Divide Basin, White Mountain, Little Colorado, and Salt Wells Creek Wild Horse Herd Management Areas (HMA) and in an area known as the North Baxter/Jack Morrow area (outside the HMAs).

  20. Wild genius - domestic fool? Spatial learning abilities of wild and domestic guinea pigs

    OpenAIRE

    Sachser Norbert; Pickel Thorsten; Lewejohann Lars; Kaiser Sylvia

    2010-01-01

    Abstract Background Domestic animals and their wild relatives differ in a wide variety of aspects. The process of domestication of the domestic guinea pig (Cavia aperea f. porcellus), starting at least 4500 years ago, led to changes in the anatomy, physiology, and behaviour compared with their wild relative, the wild cavy, Cavia aperea. Although domestic guinea pigs are widely used as a laboratory animal, learning and memory capabilities are often disregarded as being very scarce. Even less i...

  1. Hellsgate Winter Range Mitigation Project; Long-term Management Plan, Project Report 1993, Final Draft.

    Energy Technology Data Exchange (ETDEWEB)

    Berger, Matthew T.

    1994-01-01

    A study was conducted on the Hellsgate Winter Range Mitigation Project area, a 4,943 acre ranch purchased for mitigating some habitat losses associated with the original construction of Grand Coulee Dam and innundation of habitat by Lake Roosevelt. A Habitat Evaluation Procedure (HEP) study was used to determine habitat quality and quantity baseline data and future projections. Target species used in the study were sharp-tailed grouse (Tympanuchus phasianellus), mule deer (Odocoileus hemoinus), mink (Mustela vison), spotted sandpiper (Actiius colchicus), bobcat (Felis reufs), blue grouse (Dendragapus obscurus), and mourning dove (Zenaida macroura). From field data collected, limiting life values or HSI's (Habitat Suitability Index's) for each indicator species was determined for existing habitats on project lands. From this data a long term management plan was developed. This report is designed to provide guidance for the management of project lands in relation to the habitat cover types discussed and the indicator species used to evaluate these cover types. In addition, the plan discusses management actions, habitat enhancements, and tools that will be used to enhance, protect and restore habitats to desired conditions. Through planned management actions biodiversity and vegetative structure can be optimized over time to reduce or eliminate, limiting HSI values for selected wildlife on project lands.

  2. Crop-to-wild gene flow and its fitness consequences for a wild fruit tree: Towards a comprehensive conservation strategy of the wild apple in Europe.

    Science.gov (United States)

    Feurtey, Alice; Cornille, Amandine; Shykoff, Jacqui A; Snirc, Alodie; Giraud, Tatiana

    2017-02-01

    Crop-to-wild gene flow can reduce the fitness and genetic integrity of wild species. Malus sylvestris , the European crab-apple fruit tree in particular, is threatened by the disappearance of its habitat and by gene flow from its domesticated relative , Malus domestica . With the aims of evaluating threats for M. sylvestris and of formulating recommendations for its conservation, we studied here, using microsatellite markers and growth experiments: (i) hybridization rates in seeds and trees from a French forest and in seeds used for replanting crab apples in agrosystems and in forests, (ii) the impact of the level of M. domestica ancestry on individual tree fitness and (iii) pollen dispersal abilities in relation to crop-to-wild gene flow. We found substantial contemporary crop-to-wild gene flow in crab-apple tree populations and superior fitness of hybrids compared to wild seeds and seedlings. Using paternity analyses, we showed that pollen dispersal could occur up to 4 km and decreased with tree density. The seed network furnishing the wild apple reintroduction agroforestry programmes was found to suffer from poor genetic diversity, introgressions and species misidentification. Overall, our findings indicate supported threats for the European wild apple steering us to provide precise recommendations for its conservation.

  3. Chemical composition of oils from wild almond (Prunus scoparia and wild pistachio (Pistacia atlantica

    Directory of Open Access Journals (Sweden)

    Jafari Mohammadi, S. A.

    2013-03-01

    Full Text Available The aim of this study was to determine the fatty acids, sterols and triacylglycerol compositions as well as the amount of tocopherols, total phenols and pigments wild almond and cold pressed wild pistachio oils. Triacylglycerols, tocopherols and pigments were analyzed with HPLC, fatty acids and sterols with gas chromatography, and total phenols photometrically. The main fatty acids in both samples were oleic, linoleic and palmitic acids. The most predominant TAG species are SLL + PLO (21.83% in wild pistachio oil and OOO (47.27% in wild almond oil. Pheophytin a was the major pigment in wild pistachio oil. There were no pigments detected in wild almond oil. Total phenols were 57.6 mg kg-1 oil for wild pistachio and 45.3 mg kg-1 oil for wild almond oil.El objetivo de este estudio fue determinar la composición en ácidos grasos, esteroles, triglicéridos, así como tocoferoles, fenoles totales y pigmentos de aceites de almendras y pistachos silvestres prensados en frío. Triglicéridos (TAG, tocoferoles y pigmentos se analizaron mediante HPLC, los ácidos grasos y esteroles mediante cromatografía de gases, y los fenoles totales espectrofotométricamente. Los principales ácidos grasos de ambas especies fueron los ácidos oleico, linoleico y palmítico. Las especies de TAG predominantes son SLL + OLP (21,83% en el pistacho silvestre y OOO (47,27% en almendras silvestre. Feofitina a es un pigmento importante en los aceites de pistacho silvestre. No se detectó pigmentos en los aceites de almendras silvestres. Los fenoles totales fueron 57,6 mg kg-1 y 45,3 mg kg-1 en los aceites de pistacho silvestre y de almendra silvestre respectivamente.

  4. Fitness of crop-wild hybrid sunflower under competitive conditions: implications for crop-to-wild introgression.

    Science.gov (United States)

    Mercer, Kristin L; Emry, D Jason; Snow, Allison A; Kost, Matthew A; Pace, Brian A; Alexander, Helen M

    2014-01-01

    Understanding the likelihood and extent of introgression of novel alleles in hybrid zones requires comparison of lifetime fitness of parents and hybrid progeny. However, fitness differences among cross types can vary depending on biotic conditions, thereby influencing introgression patterns. Based on past work, we predicted that increased competition would enhance introgression between cultivated and wild sunflower (Helianthus annuus) by reducing fitness advantages of wild plants. To test this prediction, we established a factorial field experiment in Kansas, USA where we monitored the fitness of four cross types (Wild, F1, F2, and BCw hybrids) under different levels of interspecific and intraspecific competition. Intraspecific manipulations consisted both of density of competitors and of frequency of crop-wild hybrids. We recorded emergence of overwintered seeds, survival to reproduction, and numbers of seeds produced per reproductive plant. We also calculated two compound fitness measures: seeds produced per emerged seedling and seeds produced per planted seed. Cross type and intraspecific competition affected emergence and survival to reproduction, respectively. Further, cross type interacted with competitive treatments to influence all other fitness traits. More intense competition treatments, especially related to density of intraspecific competitors, repeatedly reduced the fitness advantage of wild plants when considering seeds produced per reproductive plant and per emerged seedling, and F2 plants often became indistinguishable from the wilds. Wild fitness remained superior when seedling emergence was also considered as part of fitness, but the fitness of F2 hybrids relative to wild plants more than quadrupled with the addition of interspecific competitors and high densities of intraspecific competitors. Meanwhile, contrary to prediction, lower hybrid frequency reduced wild fitness advantage. These results emphasize the importance of taking a full life cycle

  5. Fitness of crop-wild hybrid sunflower under competitive conditions: implications for crop-to-wild introgression.

    Directory of Open Access Journals (Sweden)

    Kristin L Mercer

    Full Text Available Understanding the likelihood and extent of introgression of novel alleles in hybrid zones requires comparison of lifetime fitness of parents and hybrid progeny. However, fitness differences among cross types can vary depending on biotic conditions, thereby influencing introgression patterns. Based on past work, we predicted that increased competition would enhance introgression between cultivated and wild sunflower (Helianthus annuus by reducing fitness advantages of wild plants. To test this prediction, we established a factorial field experiment in Kansas, USA where we monitored the fitness of four cross types (Wild, F1, F2, and BCw hybrids under different levels of interspecific and intraspecific competition. Intraspecific manipulations consisted both of density of competitors and of frequency of crop-wild hybrids. We recorded emergence of overwintered seeds, survival to reproduction, and numbers of seeds produced per reproductive plant. We also calculated two compound fitness measures: seeds produced per emerged seedling and seeds produced per planted seed. Cross type and intraspecific competition affected emergence and survival to reproduction, respectively. Further, cross type interacted with competitive treatments to influence all other fitness traits. More intense competition treatments, especially related to density of intraspecific competitors, repeatedly reduced the fitness advantage of wild plants when considering seeds produced per reproductive plant and per emerged seedling, and F2 plants often became indistinguishable from the wilds. Wild fitness remained superior when seedling emergence was also considered as part of fitness, but the fitness of F2 hybrids relative to wild plants more than quadrupled with the addition of interspecific competitors and high densities of intraspecific competitors. Meanwhile, contrary to prediction, lower hybrid frequency reduced wild fitness advantage. These results emphasize the importance of taking

  6. Fitness of Crop-Wild Hybrid Sunflower under Competitive Conditions: Implications for Crop-to-Wild Introgression

    Science.gov (United States)

    Mercer, Kristin L.; Emry, D. Jason; Snow, Allison A.; Kost, Matthew A.; Pace, Brian A.; Alexander, Helen M.

    2014-01-01

    Understanding the likelihood and extent of introgression of novel alleles in hybrid zones requires comparison of lifetime fitness of parents and hybrid progeny. However, fitness differences among cross types can vary depending on biotic conditions, thereby influencing introgression patterns. Based on past work, we predicted that increased competition would enhance introgression between cultivated and wild sunflower (Helianthus annuus) by reducing fitness advantages of wild plants. To test this prediction, we established a factorial field experiment in Kansas, USA where we monitored the fitness of four cross types (Wild, F1, F2, and BCw hybrids) under different levels of interspecific and intraspecific competition. Intraspecific manipulations consisted both of density of competitors and of frequency of crop-wild hybrids. We recorded emergence of overwintered seeds, survival to reproduction, and numbers of seeds produced per reproductive plant. We also calculated two compound fitness measures: seeds produced per emerged seedling and seeds produced per planted seed. Cross type and intraspecific competition affected emergence and survival to reproduction, respectively. Further, cross type interacted with competitive treatments to influence all other fitness traits. More intense competition treatments, especially related to density of intraspecific competitors, repeatedly reduced the fitness advantage of wild plants when considering seeds produced per reproductive plant and per emerged seedling, and F2 plants often became indistinguishable from the wilds. Wild fitness remained superior when seedling emergence was also considered as part of fitness, but the fitness of F2 hybrids relative to wild plants more than quadrupled with the addition of interspecific competitors and high densities of intraspecific competitors. Meanwhile, contrary to prediction, lower hybrid frequency reduced wild fitness advantage. These results emphasize the importance of taking a full life cycle

  7. Expression of mink cell focus-forming murine leukemia virus-related transcripts in AKR mice

    International Nuclear Information System (INIS)

    Khan, A.S.; Laigret, F.; Rodi, C.P.

    1987-01-01

    The authors used a synthetic 16-base-pair mink cell focus-forming (MCF) env-specific oligomer as radiolabeled probe to study MCF murine leukemia virus (MuLV)-related transcripts in brain, kidney, liver, spleen, and thymus tissues of AKR mice ranging from 5 weeks to 6 months (mo) of age. Tissue-specific expression of poly(A) + RNAs was seen. In addition, all the tissues tested contained 3.0-kb messages. The transcription of these MCF-related mRNAs was independent of the presence of ecotropic and xenotropic MuLVs. In general, expression of the MCF env-related transcripts appeared to peak at 2 mo of age; these messages were barely detectable in brain, kidney, liver, and spleen tissues after 2 mo and in thymus tissue after 4 mo of age. All of the subgenomic MCF env-related mRNAs appeared to contain the 190-base-pair cellular DNA insert, characteristic of the long terminal repeats associated with endogenous MCF env-related proviruses. No genomic-size (8.4-kb) transcripts corresponding to endogenous MCF-related proviruses were detected. An 8.4-kb MCF env-related mRNA was first seen at 3 mo of age, exclusively in thymus tissue. This species most likely represents the first appearance of a recombinant MCF-related MuLV genome. The transcripts which were detected in thymus tissue might be involved in the generation of leukemogenic MCF viruses

  8. Intertextuality and Intermediality in Oscar Wilde's Salome : How Oscar Wilde Become a Postmodernist

    NARCIS (Netherlands)

    de Vries, Kornelis; Bennett, Michael

    2011-01-01

    This paper approaches Wilde's play from three separate directions: intertextual, visual and musical. Comparing ideas and techniques in the play to the positions of postmodern thinkers, this chapter argues that Wilde sought to incorporate literary and philosophical elements more common to the late

  9. Bioactivities and Health Benefits of Wild Fruits

    Directory of Open Access Journals (Sweden)

    Ya Li

    2016-08-01

    Full Text Available Wild fruits are exotic or underutilized. Wild fruits contain many bioactive compounds, such as anthocyanins and flavonoids. Many studies have shown that wild fruits possess various bioactivities and health benefits, such as free radical scavenging, antioxidant, anti-inflammatory, antimicrobial, and anticancer activity. Therefore, wild fruits have the potential to be developed into functional foods or pharmaceuticals to prevent and treat several chronic diseases. In the present article, we review current knowledge about the bioactivities and health benefits of wild fruits, which is valuable for the exploitation and utilization of wild fruits.

  10. Total Mercury, Methylmercury, Inorganic Arsenic and Other Elements in Meat from Minke Whale (Balaenoptera acutorostrata) from the North East Atlantic Ocean.

    Science.gov (United States)

    Maage, Amund; Nilsen, Bente M; Julshamn, Kaare; Frøyland, Livar; Valdersnes, Stig

    2017-08-01

    Meat samples of 84 minke whales (Balaenoptera acutorostrata) mainly from the Barents Sea, collected between 1 May and 16 August 2011, were analyzed for total mercury, methylmercury, cadmium, lead, total arsenic, inorganic arsenic and selenium. The average total mercury concentration found was 0.15 ± 0.09 mg/kg, with a range from 0.05 to 0.49 mg/kg. The molar ratio of selenium to mercury varied between 1.0 and 10.3. Cadmium content ranged from 0.002 to 0.036 mg/kg, while the content of lead in whale meat ranged from whale samples exceeded established EU maximum levels for metals in fish muscle, but 4.8% and 6.8% of the samples exceeded Japanese maximum levels for total mercury and methylmercury, respectively, in whale meat. There was only minor variations in element concentrations between whales from different geographical areas, and cadmium was the only element were the concentration increased with increasing length.

  11. EvoWild: a demosimulator about wild life

    Directory of Open Access Journals (Sweden)

    Eduardo Palacio Gayoso

    2008-12-01

    Full Text Available During the last years we can see how AI (Artificial Intelligence is reappearing because of technological improvements. These improvements make possible the management of large groups of information with acceptable reply times.On the other hand, cost reductions in technology make possible that an investigation field like AI becomes to an inversion field closer to scale economies, that’s why it’ll be economically profitable to invert in this type of applications.One of the fastest consequences is the AI implantation in a big amount of devices of our environment, cell telephones, palms and of course, in the video game industry.This is the reason that took us to develop EvoWild, a simulation about wild life that has video game format and tools but at the same time implements AI algorithms like genetic algorithms and reasoning based in cases.

  12. Wild lettuce (Lactuca virosa) toxicity.

    Science.gov (United States)

    Besharat, Sima; Besharat, Mahsa; Jabbari, Ali

    2009-01-01

    Wild lettuce (Lactuca virosa) can cause toxic effects when eaten. Wild lettuce grows in the north of Iran and some natives consume it unaware of its adverse side effects. We describe eight patients with manifestations of wild lettuce toxicity, admitted to a general hospital affiliated to the Golestan University of Medical Sciences. All the patients recovered (although one had to spend 48 h in the intensive care unit) and no chronic complications were reported. A clinical suspicion of toxicity caused by wild lettuce intake and an accurate history formed the basis of the diagnosis. Conservative treatment, vital sign monitoring, control of patient intake and output, and reducing patient agitation provided the basis for treatment.

  13. Toxoplasmosis in wild and domestic animals

    Science.gov (United States)

    Toxoplasma gondii is widely distributed in wild and domestic animals. The present chapter reviews toxoplasmosis in wild and domestic animals. Coverage in wild animal species is limited to confirmed cases of toxoplasmosis, cases with parasite isolation, cases with parasite detection by PCR, and exper...

  14. Wild lettuce (Lactuca virosa) toxicity

    OpenAIRE

    Besharat, Sima; Besharat, Mahsa; Jabbari, Ali

    2009-01-01

    Wild lettuce (Lactuca virosa) can cause toxic effects when eaten. Wild lettuce grows in the north of Iran and some natives consume it unaware of its adverse side effects. We describe eight patients with manifestations of wild lettuce toxicity, admitted to a general hospital affiliated to the Golestan University of Medical Sciences. All the patients recovered (although one had to spend 48 h in the intensive care unit) and no chronic complications were reported. A clinical suspicion of toxicity...

  15. Influence of thymogene on the repair of lymphocyte DNA in the spleen of minks under the influence of chronic irradiation.; Vpliv timogehu na reparatsyiyu DNK lyimfotsityiv selezyinki norok za umov khronyichnogo opromyinennya.

    Energy Technology Data Exchange (ETDEWEB)

    Tkhorzhevs` kij, B M; Demidov, S V; Ryasenko, V Yi; Khrapunov, S M [Kievskij Gosudarstvennyj Univ., Kiev (Ukraine); [Nauchno-Proizvodstvennoe Ob` ` edinenie Pripyat` , Chernobyl (Ukraine)

    1994-12-31

    Results of own investigations of the authors as to the thymogene influence on correction of the repair system under the effect of chronic irradiation are discussed in the article. The influence of thymogene on the repair of DNA in lymphocytes of the spleen of minks has been investigated. Changes in the correlation between single- and double stranded DNA forms in a cell were determinated. It has been found that the quantity of DNA with single stranded breaks has considerably increased in animals under conditions of chronic irradiation. The use of thymogene promotes activation of repair processes in a cell.

  16. Anti-cancer and anti-oxidant efficacies of wild ginseng and cultivated wild ginseng of Korea and China

    Directory of Open Access Journals (Sweden)

    Young-Min,Ahn

    2007-02-01

    Full Text Available Objectives : The aim of this study was to verify anti-cancer and anti-oxidant efficacies of Korean wild ginseng and cultivated wild ginseng of Korea and China. Methods : For the measurement of anti-oxidation, SOD-like activity was evaluated using xanthine oxidase reduction method under in vitro environment. Subcutaneous and abdominal cancer were induced using CT-26 human colon cancer cells for the measurement of growth inhibition of cancer cells and differences in survival rate. Results : 1. Measurement of anti-oxidant activity of ginseng, Chinese and Korean cultivated wild ginseng, and natural wild ginseng samples showed concentration dependent anti-oxidant activity in HX/XOD system. Anti-oxidant activity showed drastic increase at 1mg/ml in all samples. 2. For the evaluation of growth inhibition of cancer cells after hypodermic implantation of CT-26 cancer cells in the peritoneal cavity of mice, Chinese and Korean cultivated wild ginseng and natural wild ginseng groups showed significant inhibition of tumor growth from the 12th day compared to the control group. Similar inhibitory effects were also shown on the 15th and 18th days. But there was no significant difference between the experiment groups. 3. For the observation of increase in survival rate of the natural wild ginseng group, CT-26 cancer cells were implanted in the peritoneal cavity of mice.

  17. A rapid field test for sylvatic plague exposure in wild animals

    Science.gov (United States)

    Abbott, Rachel C.; Hudak, Robert; Mondesire, Roy; Baeten, Laurie A.; Russell, Robin E.; Rocke, Tonie E.

    2014-01-01

    Plague surveillance is routinely conducted to predict future epizootics in wildlife and exposure risk for humans. The most common surveillance method for sylvatic plague is detection of antibodies to Yersinia pestis F1 capsular antigen in sentinel animals, such as coyotes (Canis latrans). Current serologic tests for Y. pestis, hemagglutination (HA) test and enzyme-linked immunosorbent assay (ELISA), are expensive and labor intensive. To address this need, we developed a complete lateral flow device for the detection of specific antibodies to Y. pestis F1 and V antigens. Our test detected anti-F1 and anti-V antibodies in serum and Nobuto filter paper samples from coyotes, and in serum samples from prairie dogs (Cynomys ludovicianus), lynx (Lynx canadensis), and black-footed ferrets (Mustela nigripes). Comparison of cassette results for anti-F1 and anti-V antibodies with results of ELISA or HA tests showed correlations ranging from 0.68 to 0.98. This device provides an affordable, user-friendly tool that may be useful in plague surveillance programs and as a research tool.

  18. Wild food plants of Remote Oceania

    Directory of Open Access Journals (Sweden)

    Will C. McClatchey

    2012-11-01

    Full Text Available Agricultural societies partly depend upon wild foods. Relationships between an agricultural society and its wild foods can be explored by examining how the society responds through colonization of new lands that have not been previously inhabited. The oldest clear example of this phenomenon took place about 5000 years ago in the tropical Western Pacific at the “boundary” interface between Near and Remote Oceania. An inventory of wild and domesticated food plants used by people living along “the remote side of ” that interface has been prepared from the literature. This was then assessed for the roles of plants at the time of original colonization of Remote Oceania. The majority of species are wild foods, and most of these are used as leafy vegetables and fruits. The wild food plants mostly serve as supplements to domesticated species, although there are a few that can be used as substitutes for traditional staples.

  19. Oscar Wilde and the brain cell.

    Science.gov (United States)

    Cohn, Elisha

    2013-01-01

    This chapter considers Oscar Wilde's interest in the brain cell as an aesthetic object. Offering an account of Wilde's career that analyzes his early interest in physiology and philosophy, this chapter argues that Wilde's uniquely aesthetic take on the brain suggests that he rejects an account of the self as autonomous or self-determining. For many late Victorians brain science threatened both the freedom of human action and the legitimacy of beauty because it had the potential to invalidate conscious experience. But writers whose work Wilde knew, like John Ruskin, W. K. Clifford, and John Tyndall, avoided the despair of materialism by using aesthetic terms in their own discussions of life's invisible materials. Wilde's art collaborates with the contemporary sciences. His depictions of the cell direct the senses to a new field of being that emphasizes the molecular life all humans have in common, in which individual responsibility and activity matter less than the necessity of beauty. © 2013 Elsevier B.V. All rights reserved.

  20. The effects of solar ultraviolet-B radiation on the growth and yield of barley are accompanied by increased DNA damage and antioxidant responses

    International Nuclear Information System (INIS)

    Mazza, C.A.; Battista, D.; Zima, A.M.; Szwarcberg-Bracchitta, M.; Giordano, C.V.; Acevedo, A.; Scopel, A.L.; Ballare, C.L.

    1999-01-01

    There is limited information on the impacts of present-day solar ultraviolet-B radiation (UV-B) on biomass and grain yield of field crops and on the mechanisms that confer tolerance to UV-B radiation under field conditions. We investigated the effects of solar UV-B on aspects of the biochemistry, growth and yield of barley crops using replicated field plots and two barley strains, a catalase (CAT)-deficient mutant (RPr 79/4) and its wild-type mother line (Maris Mink). Solar UV-B reduced biomass accumulation and grain yield in both strains. The effects on crop biomass accumulation tended to be more severe in RPr 79/4 (≈ 32% reduction) than in the mother line (≈ 20% reduction). Solar UV-B caused measurable DNA damage in leaf tissue, in spite of inducing a significant increase in UV-absorbing sunscreens in the two lines. Maris Mink responded to solar UV-B with increased CAT and ascorbate peroxidase (APx) activity. No effects of UV-B on total superoxide dismutase (SOD) activity were detected. Compared with the wild type, RPr 79/4 had lower CAT activity, as expected, but higher APx activity. Neither of these activities increased in response to UV-B in RPr 79/4. These results suggest that growth inhibition by solar UV-B involves DNA damage and oxidative stress, and that constitutive and UV-B-induced antioxidant capacity may play an important role in UV-B tolerance. (author)

  1. Wild reindeer Rangifer tarandus (L. in Chukotka

    Directory of Open Access Journals (Sweden)

    Felix B. Chernyavskii

    1998-03-01

    Full Text Available We reviewed historical records of the abundance and distribution of wild reindeer {Rangifer tarandus L. in Chukotka and studied reindeer numbers, distribution and behavior from 1983 to 1993. There were large numbers of wild reindeer in Chukotka until the end of the eighteenth century, but during the nineteenth century the population declined probably from intensive harvest after the introduction of firearms by the Cossacks. During the nineteenth century herding of domestic reindeer also increased, and reindeer herders continued to hunt wild reindeer intensively. During the 1950s there were only about 8500 wild reindeer in two separate herds in Chukotka. By the late 1970s the wild reindeer population had increased to about 11 000. Ten years later we estimated 16 534 reindeer, and found only one contiguous population. Presently, the population calves and spends the summer in the Anadyr Uplands and migrates west and southwest to spend the winter in forest tundra and northern taiga regions. Predators, primarily wolves and brown bears, kill a significant number of calves. Today, the wild reindeer in Chukotka coexist with 300 000 domestic reindeer. However, current costs of gasoline and helicopters make it prohibitive to herd reindeer in much of central Chukotka, so that wild reindeer have room for expansion. Poaching is a major conservation problem. Poachers shoot wild reindeer from helicopters to obtain velvet antlers. Leaders of domestic reindeer cooperatives encourage poaching by telling people that wild reindeer are in fact just stray domestic reindeer and there is no enforcement of game laws.

  2. Explaining the resurgent popularity of the wild: motivations for wild plant gathering in the Biosphere Reserve Grosses Walsertal, Austria.

    Science.gov (United States)

    Schunko, Christoph; Grasser, Susanne; Vogl, Christian R

    2015-06-30

    Wild plant gathering becomes again a popular and fashionable activity in Europe after gathering practices have been increasingly abandoned over the last decades. Recent ethnobotanical research documented a diversity of gathering practices from people of diverse socio-economic and cultural backgrounds who gather in urban and rural areas. Few efforts were though made to study the motivations for gathering wild plants and to understand the resurgent popularity of wild plant gathering. This paper addresses the following research questions: (1) which motivations activate wild plant gatherers? (2) which motivation-types of gatherers exist in the Grosses Walsertal? (3) how do the motivations for gathering relate to the socio-demographic background of gatherers? Field research was conducted in the Grosses Walsertal, Austria in the years 2008 and 2009 in two field research periods. Thirty-six local farmers were first interviewed with semi-structured interviews. The motivations identified in these interviews were then included in a structured questionnaire, which was used to interview 353 residents of the valley. Pupils of local schools participated in the data collection as interviewers. Principal Component Analysis was used to categorize the motivations and to identify motivation-types of wild plant gatherers. Generalized Linear Models were calculated to identify relations between motivations and the socio-demographic background of gatherers. The respondents listed 13 different motivations for gathering wild plants and four motivations for not gathering. These 17 motivations were grouped in five motivation-types of wild plant gatherers, which are in decreasing importance: product quality, fun, tradition, not-gathering, income. Women, older respondents and homegardeners gather wild plants more often for fun; older respondents gather more often for maintaining traditions; non-homegardeners more frequently mention motivations for not gathering. The resurgent popularity of

  3. Comparative functional anatomy of hindlimb muscles and bones with reference to aquatic adaptation of the sea otter.

    Science.gov (United States)

    Mori, Kent; Suzuki, Satoshi; Koyabu, Daisuke; Kimura, Junpei; Han, Sung-Yong; Endo, Hideki

    2015-05-01

    Although the sea otter (Enhydra lutris) is a complete aquatic species, spending its entire life in the ocean, it has been considered morphologically to be a semi-aquatic animal. This study aimed to clarify the unique hindlimb morphology and functional adaptations of E. lutris in comparison to other Mustelidae species. We compared muscle mass and bone measurements of five Mustelidae species: the sea otter, Eurasian river otter (Lutra lutra), American mink (Neovison vison), Japanese weasel (Mustela itatsi) and Siberian weasel (M. sibirica). In comparison with the other 4 species, E. lutris possessed significantly larger gluteus, popliteus and peroneus muscles, but smaller adductor and ischiopubic muscles. The popliteus muscle may act as a medial rotator of the crus, and the peroneus muscle may act as an abductor of the fifth toe and/or the pronator of the foot. The bundles of the gluteus superficialis muscle of E. lutris were fused with those of the tensor fasciae latae muscle and gluteofemoralis muscles, and they may play a role in femur abduction. These results suggest that E. lutris uses the abducted femur, medially rotated crus, eversion of the ankle and abducted fifth digit or extended interdigital web as a powerful propulsion generator. Therefore, we conclude that E. lutris is a complete aquatic animal, possessing differences in the proportions of the hindlimb muscles compared with those in other semi-aquatic and terrestrial mustelids.

  4. Movements and survival of black-footed ferrets associated with an experimental translocation in South Dakota

    Science.gov (United States)

    Biggins, D.E.; Godbey, J.L.; Horton, B.M.; Livieri, T.M.

    2011-01-01

    Black-footed ferrets (Mustela nigripes) apparently were extirpated from all native habitats by 1987, and their repatriation requires a combination of captive breeding, reintroductions, and translocations among sites. Improvements in survival rates of released ferrets have resulted from experience in quasi-natural environments during their rearing. Reestablishment of a self-sustaining wild population by 1999 provided the 1st opportunity to initiate new populations by translocating wild-born individuals. Using radiotelemetry, we compared behaviors and survival of 18 translocated wild-born ferrets and 18 pen-experienced captive-born ferrets after their release into a prairie dog colony not occupied previously by ferrets. Translocated wild-born ferrets moved significantly less and had significantly higher short-term survival rates than their captive-born counterparts. Using markrecapture methods, we also assessed potential impacts to the established donor population of removing 37% of its estimated annual production of kits. Annual survival rates for 30 ferret kits remaining at the donor subcomplex were higher than rates for 54 ferret kits at the control subcomplex (unmanipulated) for males (+82%) and females (+32%). Minimum survival of translocated kits did not differ significantly from survival of those at the control subcomplex. Direct translocation of young, wild-born ferrets from site to site appears to be an efficient method to establish new populations. ?? 2011 American Society of Mammalogists.

  5. WildSense: Monitoring Interactions among Wild Deer in Harsh Outdoor Environments Using a Delay-Tolerant WSN

    Directory of Open Access Journals (Sweden)

    Junho Ahn

    2016-01-01

    Full Text Available Biologists and ecologists often monitor the spread of disease among deer in the wild by using tracking systems that record their movement patterns, locations, and interaction behavior. The existing commercial systems for monitoring wild deer utilize collars with GPS sensors, deployed on captured and rereleased deer. The GPS sensors record location data every few hours, enabling researchers to approximate the interaction behavior of tracked deer with their GPS locations. However, the coarse granularity of periodically recorded GPS location data provides only limited precision for determining deer interaction behavior. We have designed a novel system to monitor wild deer interaction behavior more precisely in harsh wilderness environments. Our system combines the functionalities of both GPS and RF-radio sensors with low-cost and minimal-resource motes. We designed and built our system to be able to operate robustly for a period of up to several months for continual tracking and monitoring of the locations and interaction behaviors of wild deer in harsh environments. We successfully deployed six deer collars on six wild deer that were captured and rereleased in the Soapstone Prairie Natural Area of northern Colorado over a one-month period. In this paper, we describe how we designed and built this system and evaluate its successful operation in a wilderness area.

  6. Wild Marshmallows.

    Science.gov (United States)

    Kallas, John N.

    1984-01-01

    Provides information for teaching a unit on wild plants, including resources to use, plants to learn, safety considerations, list of plants (with scientific name, edible parts, and uses), list of plants that might cause allergic reactions when eaten. Also describes the chickweed, bull thistle, and common mallow. (BC)

  7. Commentary: Wild psychometrics: Evidence for ‘general’ cognitive performance in wild New Zealand robins, Petroica longipes

    OpenAIRE

    Hackett, Paul M. W.

    2017-01-01

    A commentary on\\ud Wild psychometrics: Evidence for ‘general’ cognitive performance in wild New Zealand robins, Petroica longipes\\ud \\ud by Shaw, R. C., Boogert, N. J., Clayton, N. S., and Burns, K. C. (2015). Anim. Behav. 109, 101–111. doi: 10.1016/j.anbehav.2015.08.001

  8. Environmental Surveillance System To Track Wild Poliovirus Transmission

    Science.gov (United States)

    Deshpande, Jagadish M.; Shetty, Sushmitha J.; Siddiqui, Zaeem A.

    2003-01-01

    Eradication of poliomyelitis from large metropolis cities in India has been difficult due to high population density and the presence of large urban slums. Three paralytic poliomyelitis cases were reported in Mumbai, India, in 1999 and 2000 in spite of high immunization coverage and good-quality supplementary immunization activities. We therefore established a systematic environmental surveillance study by weekly screening of sewage samples from three high-risk slum areas to detect the silent transmission of wild poliovirus. In 2001, from among the 137 sewage samples tested, wild poliovirus type 1 was isolated from 35 and wild poliovirus type 3 was isolated from 1. Acute flaccid paralysis (AFP) surveillance indicated one case of paralytic poliomyelitis from the city. Phylogenetic analysis with complete VP1 sequences revealed that the isolates from environmental samples belonged to four lineages of wild polioviruses recently isolated from poliomyelitis cases in Uttar Pradesh and not to those previously isolated from AFP cases in Mumbai. Wild poliovirus thus introduced caused one case of paralytic poliomyelitis. The virus was detected in environmental samples 3 months before. It was found that wild polioviruses introduced several times during the year circulated in Mumbai for a limited period before being eliminated. Environmental surveillance was found to be sensitive for the detection of wild poliovirus silent transmission. Nucleotide sequence analysis helped identify wild poliovirus reservoir areas. PMID:12732567

  9. Geographic distribution of wild potato species

    NARCIS (Netherlands)

    Hijmans, R.J.; Spooner, D.M.

    2001-01-01

    The geographic distribution of wild potatoes (Solanaceae sect. Petota) was analyzed using a database of 6073 georeferenced observations. Wild potatoes occur in 16 countries, but 88% of the observations are from Argentina, Bolivia, Mexico, and Peru. Most species are rare and narrowly endemic: for 77

  10. Wild steelhead studies. 1993 Annual report

    International Nuclear Information System (INIS)

    Holubetz, T.B.

    1995-11-01

    Significant progress was attained in implementing the complex and challenging studies of wild steelhead Oncorhynchus mykiss production in Idaho. Study sites were selected and techniques were developed to collect the needed data in remote wilderness locations. Cursory examination of existing data provides indication that most wild steelhead stocks are under escaped, especially the Group B stocks. Abundance of wild steelhead is generally declining in recent years. The portable weir concept and electronic fish counting developed through this project have been well received by land owners and reviewing governmental agencies with less impact to the land, stream, and fishery resources than conventional permanent weirs

  11. Wild and semi-wild leafy vegetables used by the Maale and Ari ethnic communities in southern Ethiopia

    NARCIS (Netherlands)

    Kidane, Berhane; Maesen, van der L.J.G.; Asfaw, Zemede; Sosef, M.S.M.; Andel, van Tinde

    2015-01-01

    We studied wild and semi-wild leafy vegetables used by the Maale and Ari ethnic communities in southern Ethiopia. Quantitative and qualitative ethnobotanical methods, including individual and focus group (n = 18) discussions, field observations, and individual interviews (n = 144), were used in

  12. Parasitic infections of wild rabbits and hares

    Directory of Open Access Journals (Sweden)

    Ilić Tamara

    2014-01-01

    Full Text Available The paper presents the most important parasitic infections of wild rabbits and hares, which harmful effect in this animal population is manifested as a gradual weakening of the immune system, reduction in fertility, weight loss and constant exhaustion. Order of Lagomorpha (hares or lagomorphs belongs to superorder of higher mammals which includes the family of rabbits (Leporidae which are represented in Europe as well as the family of whistleblowers (Ochotonidae which live only in North America and Northern regions of Asia. The most important representatives of Leporidae family are European hare (Lepus europeus and wild rabbit (Oryctolagus cuniculus. The most important endoparasitosis of hares and wild rabbits are: coccidiosis, encephalitozoonosis (nosemosis, toxoplasmosis, sarcocystosis, giardiasis, cryptosporidiosis, protostrongylosis, trichostrngylodosis, passalurosis, anoplocephalidosis, cysticercosis and fasciolosis. The most frequent ectoparasites of rabbits and wild hares are fleas, lice and ticks. Reduction in hare population, which is noticed in whole Europe including Serbia, is caused by changed living conditions, quantitatively and qualitatively insufficient nutrition, increased use of herbicides as well as various infectious diseases and the diseases of parasitic etiology. Since wild rabbits and hares pose a threat to health of domestic rabbits and people, knowledge of parasitic fauna of these wild animals is of extreme epizootiological and epidemiological importance.

  13. Wild mountains, wild rivers: Keeping the sacred origins

    Science.gov (United States)

    Linda Moon Stumpff

    2007-01-01

    For many indigenous peoples in North America, wild mountains and rivers and other natural formations exist as physical beings formed as part of a whole by forces that interconnect people with them. This perspective frames a discussion around an idea that expresses time and space as wrapped up in the mountain. If time is within the being of place and space within the...

  14. The wild tapered block bootstrap

    DEFF Research Database (Denmark)

    Hounyo, Ulrich

    In this paper, a new resampling procedure, called the wild tapered block bootstrap, is introduced as a means of calculating standard errors of estimators and constructing confidence regions for parameters based on dependent heterogeneous data. The method consists in tapering each overlapping block...... of the series first, the applying the standard wild bootstrap for independent and heteroscedastic distrbuted observations to overlapping tapered blocks in an appropriate way. Its perserves the favorable bias and mean squared error properties of the tapered block bootstrap, which is the state-of-the-art block......-order asymptotic validity of the tapered block bootstrap as well as the wild tapered block bootstrap approximation to the actual distribution of the sample mean is also established when data are assumed to satisfy a near epoch dependent condition. The consistency of the bootstrap variance estimator for the sample...

  15. Environmental Impact Statement for BOMARC Missile Site, McGuire AFB, New Jersey. Volume 1, Sections 1 through 8

    Science.gov (United States)

    1992-05-22

    OF C Hunted, Trapped Loizg taile weasel Mustela freenata OF, OF C Trapped Mivk Mustela vison OF, OF C Trapped striped skunk Mephitis mephitis OF, OF C... respiratory protective equipment and safety garments (including disposable coveralls and booties) to be utilized by site personnel, identify requirements of a

  16. Self-willed learning: experiments in wild pedagogy

    Science.gov (United States)

    Jickling, Bob

    2015-03-01

    This paper is comprised of written text and photographs of wild experiences that relive a series of ontological experiments. The text represents reflections on these experiences. The photographs, artistic expressions of the same experiences, have been made with a homemade pinhole camera—without a lens and viewfinder—thus demanding special sensual presence during creation. The form of this experimental work is reminiscent of a lyric philosophy that seeks to engage the participant—reader of text and viewer of images—with these experiments. Component pairings are arranged for viewing with text on the left and photographs on the right. Together these parings invite participants to explore patterned resonances in the world. Implicit throughout are considerations of relationships between wildness, wild learning, and a form of wild pedagogy.

  17. Explaining the resurgent popularity of the wild: motivations for wild plant gathering in the Biosphere Reserve Grosses Walsertal, Austria

    OpenAIRE

    Schunko, Christoph; Grasser, Susanne; Vogl, Christian R.

    2015-01-01

    Background Wild plant gathering becomes again a popular and fashionable activity in Europe after gathering practices have been increasingly abandoned over the last decades. Recent ethnobotanical research documented a diversity of gathering practices from people of diverse socio-economic and cultural backgrounds who gather in urban and rural areas. Few efforts were though made to study the motivations for gathering wild plants and to understand the resurgent popularity of wild plant gathering....

  18. Mycoplasma gallopavonis in eastern wild turkeys.

    Science.gov (United States)

    Luttrell, M P; Eleazer, T H; Kleven, S H

    1992-04-01

    Serum samples and tracheal cultures were collected from eastern wild turkeys (Meleagris gallopavo sylvestris) trapped for relocation in South Carolina (USA) during 1985 to 1990. Sera were tested for Mycoplasma gallisepticum and M. synoviae by the rapid plate agglutination and hemagglutination inhibition tests and were found to be negative. Tracheal cultures were negative for all pathogenic Mycoplasma spp., including M. gallisepticum, M. synoviae, M. meleagridis, and M. iowae. However, M. gallopavonis was isolated from every group of wild turkeys tested in 1986 to 1990. These data suggest that M. gallopavonis, which is generally considered nonpathogenic, may be a common microorganism in eastern wild turkeys.

  19. Transfer of 137Cs to wild vegetables

    International Nuclear Information System (INIS)

    Ito, Nobuhiko; Natsuhori, Masahiro; Mezawa, Akane; Kawakami, Akira

    1998-01-01

    For the evaluation of internal radiation dose, it is needed to estimate the amount of radionuclide incorporated to human body using a simulation model. 137 Cesium (Cs) is easily transferred associating with food intake as well as potassium and so, Cs is an important nuclide for evaluation of internal radiation. 137 Cs concentrations in wild vegetables are higher than those of cultured vegetables and milk. Therefore, the transfer coefficients of 137 Cs from soil to wild vegetables were estimated in this study. Wild vegetables and soils of their farms were collected in the Hakkoda Mountain range of Aomori Prefecture. The levels of 137 Cs in wild vegetables were 0.42-18.35 (Bq/kg), whereas those in cabbage and spinach were 0.08 and 0.01 (Bq/kg), respectively, indicating that the Cs level is dozens to several hundreds times higher in wild vegetables than cultured ones. And the transfer coefficient was estimated as 0.003-0.94 for the former and 0.001-0.8 for the latter. On the other hand, 1 37 Cs levels of the soils on which wild vegetables grew was 28.0 Bq/kg and it was 3.9 Bq/kg for the farm soil. Furthermore, the effects of water content and pH of the soil on the transfer coefficient were studied. (M.N.)

  20. Unraveling the genetic history of the European wild goats

    Science.gov (United States)

    Ureña, I.; Ersmark, E.; Samaniego, J. A.; Galindo-Pellicena, M. A.; Crégut-Bonnoure, E.; Bolívar, H.; Gómez-Olivencia, A.; Rios-Garaizar, J.; Garate, D.; Dalén, L.; Arsuaga, J. L.; Valdiosera, C. E.

    2018-04-01

    The population history of the Iberian wild goat and the Alpine ibex has been closely related to that of humans since the Palaeolithic. Current molecular and paleontological studies differ substantially on the phylogenetic origin of the European wild goats, possibly due the loss of genetic variation through time. We investigated the phylogenetic relationship between the Alpine ibex (Capra ibex) and the Iberian wild goat (Capra pyrenaica) including different Iberian wild goat subspecies by applying ancient DNA techniques combined with Next Generation Sequencing technologies. We analysed the cytochrome b gene of the mitochondrial genome in 33 ancient and modern European wild goats from Spain and France together with publicly available genetic information of modern wild goats. This work uncovers for the first time ancient genetic information of the Iberian wild goat and the Alpine ibex, spanning a time range of approximately 40,000 years to the present. Our results suggest genetic continuity between ancient and modern populations and indicate a monophyletic origin of the Alpine ibex and the Iberian wild goat when compared to other Capra species. The monophyly of both species is in agreement with other molecular studies based only on modern populations, therefore supporting one-wave migration of wild goats into Western Europe followed by possible allopatric speciation. We observe three major clades of wild goats in Western Europe: Capra ibex, Capra pyrenaica pyrenaica and the group containing the subspecies Capra pyrenaica hispanica and Capra pyrenaica victoriae. This genetic structure recognizes the distinctiveness of the bucardo (C. p. pyrenaica) from the rest of Iberian wild goats and thus supports the idea that this group is an Evolutionary Significant Unit. The divergence time estimated here indicates an almost contemporaneous split between the three clades around 50,000-90,000 years BP.

  1. 50 CFR 12.34 - Return to the wild.

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 1 2010-10-01 2010-10-01 false Return to the wild. 12.34 Section 12.34... SEIZURE AND FORFEITURE PROCEDURES Disposal of Forfeited or Abandoned Property § 12.34 Return to the wild... the wild in suitable habitat within the historical range of the species in the United States with the...

  2. Wild-Caught Versus Farmed Fish – Consumer Perception

    Directory of Open Access Journals (Sweden)

    Tomić Marina

    2017-06-01

    Full Text Available We have limited knowledge of determinants of consumer preferences for wild-caught versus farmed-raised fish, so this work aims to investigate the impact of sociodemographics, habits and frequency of fresh fish consumption, such as involvement in cooking, on the preferences for wild versus farmed fish. A survey was done on a sample of 1151 fish consumers in Croatia. Results showed that female, older consumers, consumers with higher income and those living in coastal parts of Croatia give higher preferences for wild fish and they detect differences between the taste of wild and farmed fish. Consumers with higher levels of habits of fresh fish consumption, who eat fresh fish often and are more involved in cooking, prefer wild-caught fish. These findings provide valuable information for the aquaculture sector, especially for planning marketing strategies for the promotion of farmed fish.

  3. Tartusse tuleb Wilde'i ja Vilde monument

    Index Scriptorium Estoniae

    1998-01-01

    Tartu kesklinnas Wilde Irish Pubi ees peaks 1999. a. kevadel avatama kirjanike Oscar Wilde'i ja Eduard Vilde pronkskujud. Elusuuruses kirjanikud hakkavad istuma graniitistmel. Idee autorid - pubipidaja Liam Allen, ettevõtja Marju Unt, skulptor Tiiu Kirsipuu.

  4. Research on the relativity between gene polymorphism and children cardiac insufficiency.

    Science.gov (United States)

    He, X-H; Li, C-L; Ling, N; Wang, Q-W; Wang, Z-Z; An, X-J

    2017-08-01

    We analyzed the relationship between Mink-S27 gene polymorphism and children with cardiac insufficiency. From April 2013 to April 2015, we enrolled 73 cases of children with cardiac insufficiency for this study, and all 73 were placed in the observation group. 76 normal cases were selected for the control group. Restriction fragment length polymorphism (RFLP) was used to make polymorphism analysis of the Mink-S27. Our results showed no significant differences in Mink-S27 genotype and allele distribution in both observation and control groups (p>0.05). In lesion samples collected from children with cardiac insufficiency, we detected significant difference in AA, CC genotype frequency and allele frequency between the observation group and the control group (prelatively high. GNAS2 gene polymorphism was associated with the prevalence of cardiac insufficiency in children. And also the patients' condition was correlated to the frequency of different genotypes and alleles.

  5. Wild food in Europe: a synthesis of knowledge and data of terrestrial wild food as an ecosystem service

    NARCIS (Netherlands)

    Schulp, C.J.E.; Thuiller, W.; Verburg, P.H.

    2014-01-01

    Wild food is an iconic ecosystem service that receives little attention in quantifying, valuating and mapping studies, due to the perceived low importance or due to lack of data. Here, we synthesize available data on the importance of wild food as ecosystem service, its spatial distribution and

  6. William Wilde: Historian.

    Science.gov (United States)

    Geary, L

    2016-05-01

    This essay attempts to assess William Wilde as a social historian. It examines some of his contributions to the discipline of history and looks particularly at 'The food of the Irish', which was published in the Dublin University Magazine in February 1854.

  7. Black-footed ferret areas of activity during late summer and fall at Meeteetse, Wyoming

    Science.gov (United States)

    Fagerstone, K.A.; Biggins, D.E.

    2011-01-01

    Radiotelemetry was used during 1983 and 1984 to collect information on short-term areas of activity for black-footed ferrets (Mustela nigripes) near Meeteetse, Wyoming. This population ultimately provided ferrets for the captive-breeding program that bred and released offspring into the wild since 1991. We fitted 5 adult ferrets and 13 juveniles with radiotransmitters and followed their movements during late summer and fall. Adult males had 7-day areas of activity that were >6 times as large as those of adult females. Activity areas of adult males varied little in coverage or location on a weekly basis, but females sequentially shifted their areas. Unlike juvenile females, juvenile males tended to leave their natal colonies. ?? 2011 American Society of Mammalogists.

  8. The reliability of welfare assessment according to the WelFur-protocol in the nursing period of mink (Neovison vison) is challenged by increasing welfare problems prior to weaning

    DEFF Research Database (Denmark)

    Henriksen, Britt I. F.; Møller, Steen Henrik

    2015-01-01

    The objective of this study was to test the hypothesis that the body condition of the mink dam, the frequency of dirty nests, frequency of injuries and diarrhoea change significantly with the day of assessment, post-partum, within the data collection period from parturition to weaning, influencing...... feeding', but not by enough to affect the estimated welfare classification. The score for the three other measures also varied with date of assessment but not enough to affect the classification. However, the observed change in the four measures we focused on indicates that a change in the overall Wel......Fur classification can occur if these or other measures change a little more for the better or worse. Possible solutions to this could be reducing the time window for assessment, development of a valid correction factor or to stratify the visits into an early, middle and late visit on a farm within the three...

  9. Analysis of Powdery Mildew Resistance in Wild Melon MLO Mutants

    Directory of Open Access Journals (Sweden)

    Cheng Hong

    2015-11-01

    Full Text Available Wild species have a potential value in crop breeding. Explore MLO gene which related with powdery mildew natural resistance is very important for improving the quality of melon. Resistance to powdery mildew was examined in cultivar and wild species by leaf inoculation. The wild germplasms showed resistance to powdery mildew Race1. Cloning and sequence analysis of the CmMLO2 gene identified an 85 bp difference between the wild and cultivated species. The CmMLO2 gene was expressed in the wild germplasm after fluorescence-labeled Agrobacterium-mediated transformation. A positive transgenic plant showed successful invasion by powdery mildew Race1. These results suggested that the wild species might have failed to encode the MLO protein, thereby resulting in the MLO-negative regulation of powdery mildew, which in turn resulted in the broad-spectrum resistance of the wild species to powdery mildew.

  10. [An overview of surveillance of avian influenza viruses in wild birds].

    Science.gov (United States)

    Zhu, Yun; Shi, Jing-Hong; Shu, Yue-Long

    2014-05-01

    Wild birds (mainly Anseriformes and Charadriiformes) are recognized as the natural reservoir of avian influenza viruses (AIVs). The long-term surveillance of AIVs in wild birds has been conducted in North America and Europe since 1970s. More and more surveillance data revealed that all the HA and NA subtypes of AIVs were identified in the wild ducks, shorebirds, and gulls, and the AIVs circulating in wild birds were implicated in the outbreaks of AIVs in poultry and humans. Therefore, the AIVs in wild birds pose huge threat to poultry industry and human health. To gain a better understanding of the ecology and epidemiology of AIVs in wild birds, we summarize the transmission of AIVs between wild birds, poultry, and humans, the main results of surveillance of AIVs in wild birds worldwide and methods for surveillance, and the types of samples and detection methods for AIVs in wild birds, which would be vital for the effective control of avian influenza and response to possible influenza pandemic.

  11. Final Environmental Assessment: Implementation of the Natural Resources Management Program Clear Air Force Station, Alaska

    Science.gov (United States)

    2003-06-01

    vison ), muskrat (Ondatra zibethicus), short-tail weasels ( Mustela erminea), red squirrels (Tamiasciurus hudsonicus), spruce grouse (Dendragapus...Intense or long-lasting smoke caused by large uncontrolled fire can impact air quality and seriously affect respiratory health • Destruction of valuable...canadensis), sharp-tailed grouse (Tympanuchus phasianellus), and least weasels ( Mustela nivalis). In addition to the large mammals and fur-bearers

  12. Wild and domesticated mushroom consumption in Nigeria ...

    African Journals Online (AJOL)

    African Crop Science Journal ... On the other hand, if nutrition analysis reveals different nutrition parameters for both types of mushrooms, 43.3% opted for cultivated mushroom, 42.2%, wild; 12.2% both; while 2.2% would eat ... Keywords: Consumption pattern, Lentinus squarrosulus, nutrition, perception, wild mushroom ...

  13. The absence of wild game and fish species from the USDA National Nutrient Database for standard reference: addressing information gaps in wild caught foods.

    Science.gov (United States)

    Tidball, Moira M; Tidball, Keith G; Curtis, Paul

    2014-01-01

    We highlighted gaps in nutritional data for wild game meat and wild caught fish that have a regulated harvesting season in New York State, and examined the possible role that wild game and fish play in current trends towards consumption of local, healthy meat sources. This project is part of larger study that examines family food decision-making, and explores possibilities for leveraging the locavore movement in support of consumption of wild game and fish.

  14. Lack of Mitochondrial DNA Sequence Divergence between Two Subspecies of the Siberian Weasel from Korea: Mustela sibirica coreanus from the Korean Peninsula and M. s. quelpartis from Jeju Island

    Directory of Open Access Journals (Sweden)

    Hung Sun Koh

    2012-04-01

    Full Text Available The objective of this study was to determine the degree of mitochondrial DNA (mtDNA divergence between two subspecies of Mustela sibirica from Korea (M. s. coreanus on the Korean Peninsula and M. s. quelpartis on Jeju Island and to examine the taxonomic status of M. s. quelpartis. Thus, we obtained complete sequences of mtDNA cytochrome b gene (1,140 bp from the two subspecies, and these sequences were compared to a corresponding haplotype of M. s. coreanus, downloaded from GenBank. From this analysis, it was observed that the sequences from monogenic M. s. quelpartis on Jeju Island were identical to the sequences of four M. s. coreanus from four locations across the Korean Peninsula, and that the two subspecies formed a single clade; the average nucleotide distance between the two subspecies was 0.26% (range, 0.00 to 0.53%. We found that the subspecies quelpartis is not genetically distinct from the subspecies coreanus, and that this cytochrome b sequencing result does not support the current classification, distinguishing these two subspecies by pelage color. Further systematic analyses using morphometric characters and other DNA markers are necessary to confirm the taxonomic status of M. s. quelpartis.

  15. Ed & Os : kahe Wilde näitemäng / Mati Unt

    Index Scriptorium Estoniae

    Unt, Mati, 1944-2005

    1998-01-01

    Mati Undi näidend Eduard Vilde ja Oscar Wilde'i kohtumisest. Illustratsiooniks Tiiu Kirsipuu Tartusse Wilde Irish Pubi ette loodava skulptuurigrupi kavand. Autori märkus : Tekst on kirjutatud Kahe Wilde Pubi avamiseks ja koostatud Wilde'i ja Vilde tekstidest.

  16. Distribution of Wild and Cultivated Grapes in Turkey

    Directory of Open Access Journals (Sweden)

    Ibrahim H UZUN

    2010-12-01

    Full Text Available Turkey is one of main gene centers in the world for grapes. It is believed that cultivated grapes have their origins in Turkey and the surrounding countries. Vitis vinifera ssp sylvestris is the only wild grape species in this region. That is why Turkey has a very large amount of wild grapevine populations and grape cultivars which offer to grapevine breeders a valuable gene pool. Wild grapevines have significant characters for inducing the resistence to biotic and abiotic stress factors, such as resistance to lime, drought, pests and diseases. Turkey has over 1.600 local grape cultivars, among which the majority of them are conserved at the national grape collection vineyard in Tekirda?. They are mostly used as table grapes, dried grapes or for local consumptions. Wild grapes are distributed all over the country territory, mainly in the river basins and forests. Wild grape collection vineyards were established at some universities in Turkey. These grapevines will be screened for the resistance to biotic and abiotic stress factors.

  17. Ethnobotanical review of wild edible plants of Slovakia

    Directory of Open Access Journals (Sweden)

    Łukasz Łuczaj

    2012-11-01

    Full Text Available This paper is an ethnobotanical review of wild edible plants gathered for consumption from the 19th century to the present day, within the present borders of Slovakia. Twenty-four sources (mainly ethnographic documenting the culinary use of wild plants were analysed. The use of 106 species (over 3% of the Slovak flora has been recorded. Nowadays most of them are no longer used, or used rarely, apart from a few species of wild fruits. The most frequently used plants include the fruits of Rubus idaeus, Fragaria spp., Rubus subgenus Rubus, Vaccinium myrtillus, V. vitis-idaea, Fagus sylvatica, Corylus avellana, Prunus spinosa, Pyrus spp., Malus spp., Crataegus spp. and the leaves of Urtica dioica, Rumex acetosa, Chenopodiaceae species, Cardamine amara, Glechoma spp., Taraxacum spp. and Oxalis acetosella. The most commonly used wild food taxa are nearly identical to those used in Poland, and the same negative association of wild vegetables with famine exists in Slovakia, resulting in their near complete disappearance from the present-day diet.

  18. Vitamin D status of wild Ricord's iguanas (Cyclura ricordii) and captive and wild rhinoceros iguanas (Cyclura cornuta cornuta) in the Dominican Republic.

    Science.gov (United States)

    Ramer, Jan C; Maria, Roberto; Reichard, Tim; Tolson, Peter J; Chen, Tai C; Holick, Michael F

    2005-06-01

    Calcidiol (25-hydroxyvitamin D) values are reported for 22 wild Ricord's iguanas (Cyclura ricordii) and seven wild rhinoceros iguanas (Cyclura cornuta cornuta). Calcitriol (1,25-hydroxyvitamin D) values are reported for 12 wild Ricord's iguanas and seven wild rhinoceros iguanas. These animals were captured as part of a larger health assessment study being conducted on Ricord's iguanas in Isla Cabritos National Park, Dominican Republic. A total of 13 captive rhinoceros iguanas held outdoors at Parque Zoológico Nacional were also sampled for comparison. Mean concentrations of 25-hydroxyvitamin D were 554 nmol/L (222 ng/ml) with a range of 250-1,118 nmol/L (100-448 ng/ml) for wild Ricord's iguanas, 332 nmol/L (133 ng/ml) with a range of 260-369 nmol/L (104-148 ng/ml) for wild rhinoceros iguanas, and 317 nmol/L (127 ng/ml) with a range of 220-519 nmol/L (88-208 ng/ml) for captive rhinoceros iguanas. On the basis of these results, serum concentrations of at least 325 nmol/L (130 ng/ml) for 25-hydroxyvitamin D should be considered normal for healthy Ricord's and rhinoceros iguanas.

  19. Serosurveillance of wild deer and wild boar after the epidemic of foot-and-mouth disease in the Netherlands in 2001

    NARCIS (Netherlands)

    Elbers, A.R.W.; Dekker, A.; Dekkers, L.J.M.

    2003-01-01

    Blood samples from 140 wild deer and 208 wild boar shot in the aftermath of the epidemic of foot-and-mouth disease in the Netherlands in 2001 were examined for antibodies to foot-and-mouth disease virus. They were all negative

  20. Wild translation surfaces and infinite genus

    OpenAIRE

    Randecker, Anja

    2014-01-01

    The Gauss-Bonnet formula for classical translation surfaces relates the cone angle of the singularities (geometry) to the genus of the surface (topology). When considering more general translation surfaces, we observe so-called wild singularities for which the notion of cone angle is not applicable any more. In this article, we study whether there still exist relations between the geometry and the topology for translation surfaces with wild singularities. By considering short saddle connectio...

  1. WILDE,OSCAR SKIN-DISEASE - ALLERGIC CONTACT-DERMATITIS

    NARCIS (Netherlands)

    NATER, JP

    During the last years of his life, Oscar Wilde (1856-1900) suffered from a suppurating otitis media as well as from an unidentified skin disease. The eruption was localized to his face, arms, chest and back and itched severely. A new theory is suggested, based on the fact that Wilde almost certainly

  2. Sampling wild species to conserve genetic diversity

    Science.gov (United States)

    Sampling seed from natural populations of crop wild relatives requires choice of the locations to sample from and the amount of seed to sample. While this may seem like a simple choice, in fact careful planning of a collector’s sampling strategy is needed to ensure that a crop wild collection will ...

  3. WILD PIGS: BIOLOGY, DAMAGE, CONTROL TECHINQUES AND MANAGEMENT

    Energy Technology Data Exchange (ETDEWEB)

    Mayer, John; Brisbin, I. Lehr

    2009-12-31

    The existence of problems with wild pigs (Sus scrofa) is nothing new to the Western Hemisphere. Damage by these introduced animals was reported as far back as 1505 by the early Spanish colonies in the Caribbean, where wild pigs were killing the colonists cattle. Droves of these animals also ravaged cultivated crops of maize and sugarcane on islands in the West Indies during this same time period. These wild pigs reportedly were very aggressive and often attacked Spanish soldiers hunting rebellious Indians or escaped slaves on these islands, especially when these animals were cornered. The documentation of such impacts by introduced populations of this species in the United States has subsequently increased in recent years, and continued up through the present (Towne and Wentworth. 1950, Wood and Barrett 1979, Mayer and Brisbin 1991, Dickson et al. 2001). In spite of a fairly constant history in this country since the early 1900s, wild pigs have had a dramatic recent increase in both distribution and numbers in the United States. Between 1989 and 2009, the number of states reporting the presence of introduced wild pigs went from 19 up to as many as 44. This increase, in part natural, but largely manmade, has caused an increased workload and cost for land and resource managers in areas where these new populations are found. This is the direct result of the damage that these introduced animals do. The cost of both these impacts and control efforts has been estimated to exceed a billion dollars annually (Pimentel 2007). The complexity of this problem has been further complicated by the widespread appeal and economic potential of these animals as a big game species (Tisdell 1982, Degner 1989). Wild pigs are a controversial problem that is not going away and will likely only get worse with time. Not only do they cause damage, but wild pigs are also survivors. They reproduce at a rate faster than any other mammal of comparable size, native or introduced; they can eat just

  4. Quantifying the economic contribution of wild food harvests to rural livelihoods

    DEFF Research Database (Denmark)

    Hickey, Gordon M.; Pouliot, Mariéve; Smith-Hall, Carsten

    2016-01-01

    policies. We used household income data from 7975 households in 24 developing countries across three continents collected by the Poverty Environment Network (PEN). We found 77% of households to be engaged in wild food collection from forest and non-forest environments even though the share of wild food...... regional variation in determinants and the direction of significant relationships indicate there is no one-size-fits-all approach to integrating wild foods into food and forest policies. However, our results reveal potential to increase household food security by integrating wild foods into national food......This paper empirically quantifies and analyses (i) the economic contribution of wild foods to rural households, (ii) the household socio-economic, demographic, and geographical correlates of wild food income, and (iii) how wild foods can be better incorporated into integrative food security...

  5. On the olfactory anatomy in an archaic whale (Protocetidae, Cetacea) and the minke whale Balaenoptera acutorostrata (Balaenopteridae, Cetacea).

    Science.gov (United States)

    Godfrey, Stephen J; Geisler, Jonathan; Fitzgerald, Erich M G

    2013-02-01

    The structure of the olfactory apparatus is not well known in both archaic and extant whales; the result of poor preservation in most fossils and locational isolation deep within the skulls in both fossil and Recent taxa. Several specimens now shed additional light on the subject. A partial skull of an archaic cetacean is reported from the Pamunkey River, Virginia, USA. The specimen probably derives from the upper middle Eocene (Piney Point Formation) and is tentatively assigned to the Protocetidae. Uncrushed cranial cavities associated with the olfactory apparatus were devoid of sediment. CT scans clearly reveal the dorsal nasal meatus, ethmoturbinates within the olfactory recess, the cribriform plate, the area occupied by the olfactory bulbs, and the olfactory nerve tract. Several sectioned skulls of the minke whale (Balaenoptera acutorostrata) were also examined, and olfactory structures are remarkably similar to those observed in the fossil skull from the Pamunkey River. One important difference between the two is that the fossil specimen has an elongate olfactory nerve tract. The more forward position of the external nares in extant balaenopterids when compared with those of extant odontocetes is interpreted to be the result of the need to retain a functional olfactory apparatus and the forward position of the supraoccipital/cranial vertex. An increase in the distance between the occipital condyles and the vertex in balaenopterids enhances the mechanical advantage of the epaxial musculature that inserts on the occiput, a specialization that likely stabilizes the head of these enormous mammals during lunge feeding. Copyright © 2012 Wiley Periodicals, Inc.

  6. Natural outbreak of Streptococcus agalactiae (GBS) infection in wild giant Queensland grouper, Epinephelus lanceolatus (Bloch), and other wild fish in northern Queensland, Australia.

    Science.gov (United States)

    Bowater, R O; Forbes-Faulkner, J; Anderson, I G; Condon, K; Robinson, B; Kong, F; Gilbert, G L; Reynolds, A; Hyland, S; McPherson, G; Brien, J O'; Blyde, D

    2012-03-01

    Ninety-three giant Queensland grouper, Epinephelus lanceolatus (Bloch), were found dead in Queensland, Australia, from 2007 to 2011. Most dead fish occurred in northern Queensland, with a peak of mortalities in Cairns in June 2008. In 2009, sick wild fish including giant sea catfish, Arius thalassinus (Rüppell), and javelin grunter, Pomadasys kaakan (Cuvier), also occurred in Cairns. In 2009 and 2010, two disease epizootics involving wild stingrays occurred at Sea World marine aquarium. Necropsy, histopathology, bacteriology and PCR determined that the cause of deaths of 12 giant Queensland grouper, three wild fish, six estuary rays, Dasyatis fluviorum (Ogilby), one mangrove whipray, Himantura granulata (Macleay), and one eastern shovelnose ray, Aptychotrema rostrata (Shaw), was Streptococcus agalactiae septicaemia. Biochemical testing of 34 S. agalactiae isolates from giant Queensland grouper, wild fish and stingrays showed all had identical biochemical profiles. The 16S rRNA gene sequences of isolates confirmed all isolates were S. agalactiae; genotyping of selected S. agalactiae isolates showed the isolates from giant Queensland grouper were serotype Ib, whereas isolates from wild fish and stingrays closely resembled serotype II. This is the first report of S. agalactiae from wild giant Queensland grouper and other wild tropical fish and stingray species in Queensland, Australia. © 2012 Blackwell Publishing Ltd and State of Queensland.

  7. Wild bees enhance honey bees’ pollination of hybrid sunflower

    Science.gov (United States)

    Greenleaf, Sarah S.; Kremen, Claire

    2006-01-01

    Pollinators are required for producing 15–30% of the human food supply, and farmers rely on managed honey bees throughout the world to provide these services. Yet honey bees are not always the most efficient pollinators of all crops and are declining in various parts of the world. Crop pollination shortages are becoming increasingly common. We found that behavioral interactions between wild and honey bees increase the pollination efficiency of honey bees on hybrid sunflower up to 5-fold, effectively doubling honey bee pollination services on the average field. These indirect contributions caused by interspecific interactions between wild and honey bees were more than five times more important than the contributions wild bees make to sunflower pollination directly. Both proximity to natural habitat and crop planting practices were significantly correlated with pollination services provided directly and indirectly by wild bees. Our results suggest that conserving wild habitat at the landscape scale and altering selected farm management techniques could increase hybrid sunflower production. These findings also demonstrate the economic importance of interspecific interactions for ecosystem services and suggest that protecting wild bee populations can help buffer the human food supply from honey bee shortages. PMID:16940358

  8. Wild bees enhance honey bees' pollination of hybrid sunflower.

    Science.gov (United States)

    Greenleaf, Sarah S; Kremen, Claire

    2006-09-12

    Pollinators are required for producing 15-30% of the human food supply, and farmers rely on managed honey bees throughout the world to provide these services. Yet honey bees are not always the most efficient pollinators of all crops and are declining in various parts of the world. Crop pollination shortages are becoming increasingly common. We found that behavioral interactions between wild and honey bees increase the pollination efficiency of honey bees on hybrid sunflower up to 5-fold, effectively doubling honey bee pollination services on the average field. These indirect contributions caused by interspecific interactions between wild and honey bees were more than five times more important than the contributions wild bees make to sunflower pollination directly. Both proximity to natural habitat and crop planting practices were significantly correlated with pollination services provided directly and indirectly by wild bees. Our results suggest that conserving wild habitat at the landscape scale and altering selected farm management techniques could increase hybrid sunflower production. These findings also demonstrate the economic importance of interspecific interactions for ecosystem services and suggest that protecting wild bee populations can help buffer the human food supply from honey bee shortages.

  9. Low genetic diversity of the endangered Indian wild ass Equus ...

    Indian Academy of Sciences (India)

    DEVENDRA KHAIRE

    (Equus hemionus khur) belongs to an endangered wild species/subspecies of wild ... species of the Asiatic wild asses, E. hemionus and E. kiang, have been described on .... 57.9. Tseng et al. (2010). 5 -TCA CCC ACT AAA TCT CAA ATC C-3.

  10. Genetic Diversity of Wild Rice Species in Yunnan Province of China

    OpenAIRE

    Zai-quan CHENG; Fu-you YING; Ding-qing LI; Teng-qiong YU; Jian FU; Hui-jun YAN; Qiao-fang ZHONG; Dun-yu ZHANG; Wei-jiao LI; Xing-qi HUANG

    2012-01-01

    Yunnan Province of China is one of the important centers for origin and evolution of cultivated rice worldwide. Wild rice is the ancestor of the cultivated rice. Many elite traits of wild rice have widened the genetic basis in cultivated rice. However, many populations of wild rice species have disappeared in the past few years. Therefore, the current status of wild rice resources should be updated and the genetic diversity of wild rice species should be examined for further germplasm preserv...

  11. Hybrids between cultivated and wild carrots in natural populations in Denmark

    DEFF Research Database (Denmark)

    Magnussen, L.S.; Hauser, Thure Pavlo

    2007-01-01

    Many cultivated plant species are able to hybridize with related wild plants. However, it is not clear whether their hybrids are able to survive and reproduce outside managed fields, and if cultivar genes introgress into wild populations. In areas where wild carrots co-occur with carrot root......-crops, pollen and seeds may flow from two different sources in the fields to the surrounding wild populations: from pure cultivar plants that occasionally flower, and from flowering 'bolters' that originate from hybridizations between wild (male) and cultivated carrots (female) in seed production fields...... by AFLP. Four hybrids were identified among the 71 plants analysed, and these were most likely F(2) or backcross individuals, sired by pollen from hybrid bolters. Wild populations close to fields were genetically somewhat more similar to cultivars than wild populations far from fields, suggesting...

  12. Wild and Scenic Rivers

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — This map layer portrays the linear federally-owned land features (i.e., national parkways, wild and scenic rivers, etc.) of the United States, Puerto Rico, and the...

  13. Spatial distribution and risk factors of Brucellosis in Iberian wild ungulates

    Directory of Open Access Journals (Sweden)

    de la Fuente José

    2010-03-01

    Full Text Available Abstract Background The role of wildlife as a brucellosis reservoir for humans and domestic livestock remains to be properly established. The aim of this work was to determine the aetiology, apparent prevalence, spatial distribution and risk factors for brucellosis transmission in several Iberian wild ungulates. Methods A multi-species indirect immunosorbent assay (iELISA using Brucella S-LPS antigen was developed. In several regions having brucellosis in livestock, individual serum samples were taken between 1999 and 2009 from 2,579 wild bovids, 6,448 wild cervids and4,454 Eurasian wild boar (Sus scrofa, and tested to assess brucellosis apparent prevalence. Strains isolated from wild boar were characterized to identify the presence of markers shared with the strains isolated from domestic pigs. Results Mean apparent prevalence below 0.5% was identified in chamois (Rupicapra pyrenaica, Iberian wild goat (Capra pyrenaica, and red deer (Cervus elaphus. Roe deer (Capreolus capreolus, fallow deer (Dama dama, mouflon (Ovis aries and Barbary sheep (Ammotragus lervia tested were seronegative. Only one red deer and one Iberian wild goat resulted positive in culture, isolating B. abortus biovar 1 and B. melitensis biovar 1, respectively. Apparent prevalence in wild boar ranged from 25% to 46% in the different regions studied, with the highest figures detected in South-Central Spain. The probability of wild boar being positive in the iELISA was also affected by age, age-by-sex interaction, sampling month, and the density of outdoor domestic pigs. A total of 104 bacterial isolates were obtained from wild boar, being all identified as B. suis biovar 2. DNA polymorphisms were similar to those found in domestic pigs. Conclusions In conclusion, brucellosis in wild boar is widespread in the Iberian Peninsula, thus representing an important threat for domestic pigs. By contrast, wild ruminants were not identified as a significant brucellosis reservoir for

  14. Hybridization between crops and wild relatives: the contribution of cultivated lettuce to the vigour of crop–wild hybrids under drought, salinity and nutrient deficiency conditions

    OpenAIRE

    Uwimana, Brigitte; Smulders, Marinus J.M.; Hooftman, Danny A.P.; Hartman, Yorike; van Tienderen, Peter H.; Jansen, Johannes; McHale, Leah K.; Michelmore, Richard W.; van de Wiel , Clemens C.M.; Visser , Richard G.F.

    2012-01-01

    With the development of transgenic crop varieties, crop–wild hybridization has received considerable consideration with regard to the potential of transgenes to be transferred to wild species. Although many studies have shown that crops can hybridize with their wild relatives and that the resulting hybrids may show improved fitness over the wild parents, little is still known on the genetic contribution of the crop parent to the performance of the hybrids. In this study, we investigated the v...

  15. wild and domesticated mushroom consumption in nigeria abstract

    African Journals Online (AJOL)

    USER

    of awareness, consumption history, wild or ... 15.6% were students and 11.1% business ... 1.1. N/A. 5. 5.6. NA = Not applicable mushrooms for over 10 years; whereas 13.3% ... Consumption history of wild and cultivated mushrooms in Nigeria.

  16. Plant Fitness Assessment for Wild Relatives of Insect Resistant Bt-Crops

    Directory of Open Access Journals (Sweden)

    D. K. Letourneau

    2012-01-01

    Full Text Available When field tests of transgenic plants are precluded by practical containment concerns, manipulative experiments can detect potential consequences of crop-wild gene flow. Using topical sprays of bacterial Bacillus thuringiensis larvicide (Bt and larval additions, we measured fitness effects of reduced herbivory on Brassica rapa (wild mustard and Raphanus sativus (wild radish. These species represent different life histories among the potential recipients of Bt transgenes from Bt cole crops in the US and Asia, for which rare spontaneous crosses are expected under high exposure. Protected wild radish and wild mustard seedlings had approximately half the herbivore damage of exposed plants and 55% lower seedling mortality, resulting in 27% greater reproductive success, 14-day longer life-spans, and 118% more seeds, on average. Seed addition experiments in microcosms and in situ indicated that wild radish was more likely to spread than wild mustard in coastal grasslands.

  17. Hybridization between crops and wild relatives: the contribution of cultivated lettuce to the vigour of crop-wild hybrids under drought, salinity and nutrient deficiency conditions

    NARCIS (Netherlands)

    Uwimana, B.; Smulders, M.J.M.; Hooftman, D.A.P.; Hartman, Y.; Tienderen, van P.H.; Jansen, J.; McHale, L.K.; Michelmore, R.W.; Wiel, van de C.C.M.; Visser, R.G.F.

    2012-01-01

    With the development of transgenic crop varieties, crop–wild hybridization has received considerable consideration with regard to the potential of transgenes to be transferred to wild species. Although many studies have shown that crops can hybridize with their wild relatives and that the resulting

  18. Hybridization between crops and wild relatives: the contribution of cultivated lettuce to the vigour of crop-wild hybrids under drought, salinity and nutrient deficiency conditions

    NARCIS (Netherlands)

    Uwimana, B.; Smulders, M.J.M.; Hooftman, D.A.P.; Hartman, Y.; van Tienderen, P.H.; Jansen, J.; McHale, L.K.; Michelmore, R.W.; van de Wiel, C.C.M.; Visser, R.G.F.

    2012-01-01

    With the development of transgenic crop varieties, crop-wild hybridization has received considerable consideration with regard to the potential of transgenes to be transferred to wild species. Although many studies have shown that crops can hybridize with their wild relatives and that the resulting

  19. Assessing landowners' attitudes toward wild hogs and support for control options.

    Science.gov (United States)

    Caplenor, Carlotta A; Poudyal, Neelam C; Muller, Lisa I; Yoest, Chuck

    2017-10-01

    Wild hogs (Sus scrofa) are an invasive species with destructive habits, particularly rooting and wallowing, which can directly impact agricultural crops, pasture land, and water quality. Considering wild hogs are widely dispersed across the landscape, they are extremely difficult to control. Disagreements can arise among different stakeholders over whether and how their populations should be managed. The purpose of this article was to examine Tennessee, United States landowners' attitudes toward wild hogs, to compare acceptability of control methods, and to evaluate factors significantly influencing public support for regulations to control wild hogs. Logistic regression was employed to analyze data collected from a statewide survey of rural landowners in the fall of 2015. Landowners had overwhelmingly negative attitudes towards wild hogs, and were concerned about their impact on the natural environment and rural economy. Although landowners showed support for controlling wild hogs, levels of acceptability for management options varied. Respondents favored active management and supported education and incentive-based control programs to control wild hogs. Cognitive concepts such as social and personal norms and awareness of consequences, as well as demographic characteristics, significantly predicted landowners' support for state regulations to control wild hogs in Tennessee. Findings increase our understanding of the human dimensions of wild hog management and that of other similarly invasive animals, and may guide resource managers in designing effective and socially acceptable management strategies to control wild hog populations in Tennessee and elsewhere. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. WILD BEES (HYMENOPTERA: APOIDEA AS BIOINDICATORS IN THE NEOTROPICS

    Directory of Open Access Journals (Sweden)

    Enrique Reyes-Novelo

    2008-12-01

    Full Text Available The present is a review about the use of wild bees as an indicator group in biodiversity and fragmentation studies. It describes the criteria used for the selection of bioindicator groups and it discusses the available information to evaluate if wild bees meet this criteria. The reviewed information suggests that wild bees comply with the requeriments for a suitable bioindicator group. Its use is recommended for Neotropical ecosystems.

  1. Haemoglobin polymorphism in wild and cultured African catfish ...

    African Journals Online (AJOL)

    Haemoglobin polymorphism, haemoglobin concentration, blood group and genotypes of wild and cultured Clarias gariepinus were investigated. Blood samples of Clarias gariepinus collected from Lake Alau (wild) and Dalori fish farm (cultured) were subjected to cellulose acetate electrophoresis to reveal the activities of ...

  2. The control of classical swine fever in wild boar

    Directory of Open Access Journals (Sweden)

    Volker eMoennig

    2015-11-01

    Full Text Available Classical swine fever (CSF is a viral disease with severe economic consequences for domestic pigs. Natural hosts for the CSF virus (CSFV are members of the family Suidae, i.e. Eurasian wild boar (sus scrofa are also susceptible. CSF in wild boar poses a serious threat to domestic pigs. CSFV is an enveloped RNA virus belonging to the pestivirus genus of the Flaviviridae family. Transmission of the infection is usually by direct contact or by feeding of contaminated meat products. In recent decades CSF has been successfully eradicated from Australia, North America, and the European Union. In areas with dense wild boar populations CSF tends to become endemic whereas it is often self-limiting in small, less dense populations. In recent decades eradication strategies of CSF in wild boar have been improved considerably. The reduction of the number of susceptible animals to a threshold level where the basic reproductive number is R0<1 is the major goal of all control efforts. Depending on the epidemiological situation, hunting measures combined with strict hygiene may be effective in areas with a relatively low density of wild boar. Oral immunization was shown to be highly effective in endemic situations in areas with a high density of wild boar.

  3. Wild food plants and wild edible fungi of Heihe valley (Qinling Mountains, Shaanxi, central China: herbophilia and indifference to fruits and mushrooms

    Directory of Open Access Journals (Sweden)

    Yongxiang Kang

    2012-12-01

    Full Text Available The aim of the study was to investigate knowledge and use of wild food plants and fungi in Han (i.e. Chinese nationality villages in central China, including famine plants used in the respondents' childhood. A valley adjacent to the extremely species-rich temperate forest vegetation of the Taibai Nature Reserve was chosen. Eighty-two people from 5 villages took part in the study. Altogether, 159 wild food plant species and 13 fungi folk taxa were mentioned by informants. The mean number of freelisted wild foods was very high (24.8; median – 21.5. An average respondent listed many species of wild vegetables (mean – 17, me- dian – 14.5, a few wild fruits (mean – 5.9 and median – 6 and very few fungi (mean – 1.9, median – 1, which they had eaten. Over 50% of respondents mentioned gathering the young shoots or leaves of Celastrus orbiculatus, Staphylea bumalda and S. holocapra, Caryopteris divaricata, Helwingia japonica, Pteridium aquilinum, Pimpinella sp., Amaranthus spp., Matteucia struthiopteris, Allium spp., Cardamine macrophylla and Chenopodium album. Only one species of fruits (Schisandra sphenanthera and none of the mushrooms were mentioned by over half of the respondents. Although very diverse, it can be noted that the use of wild vegetables has decreased compared to the second half of the 20th century, as informants listed several plants which they had stopped using (e.g. Abelia engleriana due to the availability of cultivated vegetables and other foodstuffs. On the other hand, the collection of the most well-known wild vegetables is maintained by selling them to tourists visiting agritourist farms, and restaurants.

  4. Bacteriological and genetic assessment of game meat from Japanese wild boars.

    Science.gov (United States)

    Naya, Yuka; Horiuchi, Motohiro; Ishiguro, Naotaka; Shinagawa, Morikazu

    2003-01-15

    Bacterial tests were used to assess bacterial contamination of game meat from Japanese wild boars. The bacterial contamination of wild boar meat was less than that of domestic pork, as determined by aerobic plate counts (APC) and coliform counts. None of the meat examined in this study was contaminated by Salmonella or E. coli O-157. To detect adulteration by domestic pig meat or European wild boar meat, 46 samples of game meat sold as Japanese wild boar were examined genetically. A total of 17 samples showed genetic haplotypes of European and Asian domestic pigs in the D-loop of mitochondrial DNA (mtDNA), and 16 samples showed nuclear glucosephosphate isomerase-processed pseudogene (GPIP) genotypes of European domestic pigs. The European GPIP genotypes of these samples were confirmed by PCR-RFLP analysis. These results indicate that some game meat sold as Japanese wild boar is adulterated by cross-breeding between pigs and wild boars or by contamination with meat from domestic pigs or European wild boars.

  5. Modelling of a Batch Whey Cultivation of Kluyveromyces marxianus var. lactis MC 5 with Investigation of Mass Transfer Processes in the Bioreactor

    Directory of Open Access Journals (Sweden)

    Mitko Petrov

    2015-04-01

    Full Text Available This study presents a mathematical model of a batch fermentation of lactose oxidation from a natural substratum in a cultivation by the strain Kluyweromyces marxianus var. lactis MC 5. In the model of the process, the mass transfer in the bioreactor for oxygen concentration in the gas phase (GP and in the liquid phase (LP is based on the dispersion model of the GP. In addition, perfect mixing in LP is included. Nine models were investigated for specific growth rate and specific oxygen consumptions rate: Monod, Mink, Tessier, Aiba, Andrews, Haldane, Luong, Edward and Han-Levenspiel. In regard to the parameter estimation, the worst observed error was used for all experiments as an objective function. This approach is a special case of multi objective parameter estimation problems allowing the parameter estimation problem to become a min-max problem. The results obtained (values of criteria, relative error and statistics λ for the specific growth rate showed that the best fit to experimental data is achieved when applying the Mink model. In a combination a Mink, and Monod, Mink, Luong, Haldane, and Han-Levenspiel are used for specific oxygen consumptions rate. Based on the investigation, it was discovered that the best fit belonged to the models of Mink and Haldane, Mink and Luong and Mink and Han-Levenspiel. Therefore, these particular models are used for modeling the batch processes.

  6. Estimation of in situ mating systems in wild sorghum (Sorghum ...

    Indian Academy of Sciences (India)

    The high outcrossing rates of wild/weedy sorghum populations in Ethiopia indicate a high potential for crop genes (including transgenes) to spread within the wild pool. Therefore, effective risk management strategies may be needed if the introgression of transgenes or other crop genes from improved cultivars into wild or ...

  7. WILD PIG ATTACKS ON HUMANS

    Energy Technology Data Exchange (ETDEWEB)

    Mayer, J.

    2013-04-12

    Attacks on humans by wild pigs (Sus scrofa) have been documented since ancient times. However, studies characterizing these incidents are lacking. In an effort to better understand this phenomenon, information was collected from 412 wild pig attacks on humans. Similar to studies of large predator attacks on humans, data came from a variety of sources. The various attacks compiled occurred in seven zoogeographic realms. Most attacks occurred within the species native range, and specifically in rural areas. The occurrence was highest during the winter months and daylight hours. Most happened under non-hunting circumstances and appeared to be unprovoked. Wounded animals were the chief cause of these attacks in hunting situations. The animals involved were typically solitary, male and large in size. The fate of the wild pigs involved in these attacks varied depending upon the circumstances, however, most escaped uninjured. Most human victims were adult males traveling on foot and alone. The most frequent outcome for these victims was physical contact/mauling. The severity of resulting injuries ranged from minor to fatal. Most of the mauled victims had injuries to only one part of their bodies, with legs/feet being the most frequent body part injured. Injuries were primarily in the form of lacerations and punctures. Fatalities were typically due to blood loss. In some cases, serious infections or toxemia resulted from the injuries. Other species (i.e., pets and livestock) were also accompanying some of the humans during these attacks. The fates of these animals varied from escaping uninjured to being killed. Frequency data on both non-hunting and hunting incidents of wild pig attacks on humans at the Savannah River Site, South Carolina, showed quantitatively that such incidents are rare.

  8. 36 CFR 261.23 - Wild free-roaming horses and burros.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Wild free-roaming horses and... AGRICULTURE PROHIBITIONS General Prohibitions § 261.23 Wild free-roaming horses and burros. The following are prohibited: (a) Removing or attempting to remove a wild free-roaming horse or burro from the National Forest...

  9. CWRML: representing crop wild relative conservation and use data in XML.

    Science.gov (United States)

    Moore, Jonathan D; Kell, Shelagh P; Iriondo, Jose M; Ford-Lloyd, Brian V; Maxted, Nigel

    2008-02-25

    Crop wild relatives are wild species that are closely related to crops. They are valuable as potential gene donors for crop improvement and may help to ensure food security for the future. However, they are becoming increasingly threatened in the wild and are inadequately conserved, both in situ and ex situ. Information about the conservation status and utilisation potential of crop wild relatives is diverse and dispersed, and no single agreed standard exists for representing such information; yet, this information is vital to ensure these species are effectively conserved and utilised. The European Community-funded project, European Crop Wild Relative Diversity Assessment and Conservation Forum, determined the minimum information requirements for the conservation and utilisation of crop wild relatives and created the Crop Wild Relative Information System, incorporating an eXtensible Markup Language (XML) schema to aid data sharing and exchange. Crop Wild Relative Markup Language (CWRML) was developed to represent the data necessary for crop wild relative conservation and ensure that they can be effectively utilised for crop improvement. The schema partitions data into taxon-, site-, and population-specific elements, to allow for integration with other more general conservation biology schemata which may emerge as accepted standards in the future. These elements are composed of sub-elements, which are structured in order to facilitate the use of the schema in a variety of crop wild relative conservation and use contexts. Pre-existing standards for data representation in conservation biology were reviewed and incorporated into the schema as restrictions on element data contents, where appropriate. CWRML provides a flexible data communication format for representing in situ and ex situ conservation status of individual taxa as well as their utilisation potential. The development of the schema highlights a number of instances where additional standards-development may

  10. Foetal life protein restriction in male mink (Neovison vison) kits lowers post-weaning protein oxidation and the relative abundance of hepatic fructose-1,6-bisphosphatase mRNA

    DEFF Research Database (Denmark)

    Matthiesen, Connie Marianne Frank; Blache, D.; Thomsen, Preben Dybdahl

    2012-01-01

    Foetal life malnutrition has been studied intensively in a number of animal models. Results show that especially foetal life protein malnutrition can lead to metabolic changes later in life. This might be of particular importance for strict carnivores, for example, cat and mink (Neovison vison...... born to mothers fed either a low-protein diet (LP), that is, 14% of metabolizable energy (ME) from protein (foetal low – FL), n = 16, or an adequate-protein (AP) diet, that is, 29% of ME from protein (foetal adequate – FA), n = 16) in the last 16.3 ± 1.8 days of pregnancy were used. The FL offspring...... had lower birth weight and lower relative abundance of fructose-1,6-bisphosphatase (Fru-1,6-P2ase) and pyruvate kinase mRNA in foetal hepatic tissue than FA kits. The mothers were fed a diet containing adequate protein until weaning. At weaning (7 weeks of age), half of the kits from each foetal...

  11. Wild grapevine management

    Science.gov (United States)

    H. Clay Smith

    1989-01-01

    Wild grapevines are a problem for forest managers in many areas of the central hardwood forests. The vines grow on a wide range of soil and site conditions but usually are more concentrated on good sites (northern red oak site index 70 and above), on the faster growing more valuable timber. Presently there is more interest and concern in controlling grapevine for the...

  12. Consumerism, Celebrity Culture and the Aesthetic Impure in Oscar Wilde

    Directory of Open Access Journals (Sweden)

    Martino, Pierpaolo

    2015-12-01

    Full Text Available This article investigates the discursive arena in which Oscar Wilde exercised his countercultural and necessarily impure aesthetic taste, focusing on some defining aspects and texts of Wilde's epopee, namely his cult of celebrity, which was nourished, in particular, by his 1882 tour of the United States, his interest in performance – which turned the author into the director and main actor of the very drama entitled Oscar Wilde – and his apparently contradictory approach to consumer culture. Wilde, indeed, seemed to embrace opposite stances in relation to consumerism as it is witnessed by such works as «The Soul of Man Under Socialism» and The Picture of Dorian Gray, on the one side, and «The Decay of Lying», Lady Windermere's Fan and An Ideal Husband, on the other. This essay also reads Wilde's last play The Importance of Being Earnest in terms of a «performance about performance», rooted as Wilde's previous plays in consumer culture and capable of deconstructing the Victorian highly normative (and 'rational' approach to gender and, in particular, to masculinity.

  13. WildSpan: mining structured motifs from protein sequences

    Directory of Open Access Journals (Sweden)

    Chen Chien-Yu

    2011-03-01

    Full Text Available Abstract Background Automatic extraction of motifs from biological sequences is an important research problem in study of molecular biology. For proteins, it is desired to discover sequence motifs containing a large number of wildcard symbols, as the residues associated with functional sites are usually largely separated in sequences. Discovering such patterns is time-consuming because abundant combinations exist when long gaps (a gap consists of one or more successive wildcards are considered. Mining algorithms often employ constraints to narrow down the search space in order to increase efficiency. However, improper constraint models might degrade the sensitivity and specificity of the motifs discovered by computational methods. We previously proposed a new constraint model to handle large wildcard regions for discovering functional motifs of proteins. The patterns that satisfy the proposed constraint model are called W-patterns. A W-pattern is a structured motif that groups motif symbols into pattern blocks interleaved with large irregular gaps. Considering large gaps reflects the fact that functional residues are not always from a single region of protein sequences, and restricting motif symbols into clusters corresponds to the observation that short motifs are frequently present within protein families. To efficiently discover W-patterns for large-scale sequence annotation and function prediction, this paper first formally introduces the problem to solve and proposes an algorithm named WildSpan (sequential pattern mining across large wildcard regions that incorporates several pruning strategies to largely reduce the mining cost. Results WildSpan is shown to efficiently find W-patterns containing conserved residues that are far separated in sequences. We conducted experiments with two mining strategies, protein-based and family-based mining, to evaluate the usefulness of W-patterns and performance of WildSpan. The protein-based mining mode

  14. DEMAND FOR WILD BLUEBERRIES AT FARM AND PROCESSOR LEVELS

    OpenAIRE

    Cheng, Hsiang-Tai; Peavey, Stephanie R.; Kezis, Alan S.

    2000-01-01

    The wild blueberry crop harvested in Maine and eastern Canada has increased considerably in recent years. The purpose of this study is to understand the recent trends in demand for wild blueberries with particular attention to the effects of production and the marketing of wild and cultivated blueberries. A price response model was developed to analyze farm-gate price and the processor price, using annual data from 1978 through 1997. Key explanatory variables in the model include quantity of ...

  15. 19 CFR 12.29 - Plumage and eggs of wild birds.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Plumage and eggs of wild birds. 12.29 Section 12... THE TREASURY SPECIAL CLASSES OF MERCHANDISE Wild Animals, Birds, and Insects § 12.29 Plumage and eggs of wild birds. (a) The provisions of Chapter 5, Additional U.S. Note 1, relating to the plumage of...

  16. Annual Report for 2003 Wild Horse Research and Field Activities

    Science.gov (United States)

    Ransom, Jason; Singer, Francis J.; Zeigenfuss, Linda C.

    2004-01-01

    As stated in the Wild Horse Fertility Control Field Trial Plan, the Bureau of Land Management (BLM) has an immediate need for a safe, effective contraceptive agent to assist in the management of the large number of wild horses on western rangelands. The BLM and the U.S. Geological Survey-Biological Resources Discipline (USGS/BRD) are testing the immunocontraceptive agent Porcine Zonae Pellucida (PZP) in field trials with three free-roaming herds of western wild horses. Extensive research has already been conducted on the safety, efficacy, and duration of PZP applications in both domestic and feral horses on eastern barrier islands and in some select trials I with wild horses in Nevada managed by the BLM. However, significant questions remain concerning the effects of I PZP application at the population level in the wild, as well as effects at the individual level on behavior, social structure, and harem dynamics of free-ranging animals. These questions are best answered with field trials on wild horse herds under a tight research protocol. The ultimate goal is to provide the BLM with the protocols and information necessary to begin using fertility control to regulate population growth rates in wild horse herds on a broader scale. Fertility control is intended to assist the conventional capture, removal, and adoption process as a I means of controlling excess numbers of wild horses and burros, and to greatly reduce the adoption costs and numbers of animals handled. Fertility control is not intended to totally replace the removal and adoption process.

  17. Associations between biosecurity and outbreaks of canine distemper on Danish mink farms in 2012-2013

    DEFF Research Database (Denmark)

    Gregers-Jensen, Louise; Agger, Jens Frederik; Hammer, Anne Sofie Vedsted

    2015-01-01

    distemper virus. Mortality was only assessed on the case farms, and there was a non-significantly lower mortality on vaccinated farms than on the non-vaccinated farms. Furthermore, the proportion of farms with observations of wild red foxes (Vulpes vulpes) inside the farm enclosures were larger for case...

  18. Parasites of domestic and wild animals in South Africa. XLVIII . Ticks (Acari: Ixodidae infesting domestic cats and wild felids in southern Africa

    Directory of Open Access Journals (Sweden)

    Ivan G. Horak

    2010-11-01

    Full Text Available Ticks collected from domestic cats (Felis catus, cheetahs (Acinonyx jubatus,caracals (Caracal caracal, African wild cats (Felis lybica, black-footed cats (Felis nigripes, a serval (Leptailurus serval, lions(Panthera leo, and leopards (Panthera pardus were identified and counted. Thirteen species of ixodid ticks and one argasid tick were identified from domestic cats and 17 species of ixodid ticks from wild felids. The domestic cats and wild felids harboured 11 ixodid species in common. The adults of Haemaphysalis elliptica, the most abundant tick species infesting cats and wild felids, were most numerous on a domestic cat in late winter and in mid-summer, during 2 consecutive years. The recorded geographic distribution of the recently described Haemaphysalis colesbergensis, a parasite of cats and caracals, was extended by 2 new locality records in the Northern Cape Province,South Africa.

  19. Wild ideas in food

    DEFF Research Database (Denmark)

    Münke, Christopher; Halloran, Afton Marina Szasz; Vantomme, Paul

    2015-01-01

    Foraging for all manner of wild plants, animals and fungi and their products makes up part of the traditional diets of approximately 300 million worldwide (Bharucha and Pretty, 2010). Furthermore, their relevance in the global food supply is often underestimated, as policies and statistics...

  20. Into the urban wild

    DEFF Research Database (Denmark)

    Mollee, Eefke Maria; Pouliot, Mariéve; McDonald, Morag A.

    2017-01-01

    In sub-Saharan Africa, many people depend on natural resources for their livelihoods. While urbanisation causes landscape changes, little is known of how this process affects the use of wild plant resources by urban populations. This study contributes to addressing this knowledge gap by exploring...

  1. Emerging adults and the future of wild nature

    Science.gov (United States)

    Harry C. Zinn; Alan R. Graefe

    2007-01-01

    Many resource managers and wilderness advocates see links between appreciating wild nature, participating in traditional outdoor activities, and support for protecting wild areas. Some of these individuals express concern that the values and recreation behavior of today's young people may suggest less support for protecting wilderness in the future. Although...

  2. Hybridization between crops and wild relatives: the contribution of cultivated lettuce to the vigour of crop-wild hybrids under drought, salinity and nutrient deficiency conditions.

    Science.gov (United States)

    Uwimana, Brigitte; Smulders, Marinus J M; Hooftman, Danny A P; Hartman, Yorike; van Tienderen, Peter H; Jansen, Johannes; McHale, Leah K; Michelmore, Richard W; van de Wiel, Clemens C M; Visser, Richard G F

    2012-10-01

    With the development of transgenic crop varieties, crop-wild hybridization has received considerable consideration with regard to the potential of transgenes to be transferred to wild species. Although many studies have shown that crops can hybridize with their wild relatives and that the resulting hybrids may show improved fitness over the wild parents, little is still known on the genetic contribution of the crop parent to the performance of the hybrids. In this study, we investigated the vigour of lettuce hybrids using 98 F(2:3) families from a cross between cultivated lettuce and its wild relative Lactuca serriola under non-stress conditions and under drought, salinity and nutrient deficiency. Using single nucleotide polymorphism markers, we mapped quantitative trait loci associated with plant vigour in the F(2:3) families and determined the allelic contribution of the two parents. Seventeen QTLs (quantitative trait loci) associated with vigour and six QTLs associated with the accumulation of ions (Na(+), Cl(-) and K(+)) were mapped on the nine linkage groups of lettuce. Seven of the vigour QTLs had a positive effect from the crop allele and six had a positive effect from the wild allele across treatments, and four QTLs had a positive effect from the crop allele in one treatment and from the wild allele in another treatment. Based on the allelic effect of the QTLs and their location on the genetic map, we could suggest genomic locations where transgene integration should be avoided when aiming at the mitigation of its persistence once crop-wild hybridization takes place.

  3. Tactical Miniature Crystal Oscillator.

    Science.gov (United States)

    1979-12-01

    One is manufactured by Johns-Mansville, called Min-K, and the other is made in England by Micropore insulation Ltd., called Microtherm . Thermal...conductivities of both products compared to that of Vespel which had been proposed for the pedestal are as follows: Min-K Microtherm Vespel Thermal...thermal conductivities are reduced significantly in low pressure (vacuum) applications as follows: Min-K Microtherm Vespel Thermal Conductivity .09

  4. Characterization and expression of dehydrins in wild Egyptian pea ...

    African Journals Online (AJOL)

    Characterization and expression of dehydrins in wild Egyptian pea ( Pisum sativum L.) ... was isolated and characterized from wild Egyptian pea (Pisum sativum L.) ... DNA sequence indicated an open reading frame which predicts a protein ...

  5. Hunting with lead: association between blood lead levels and wild game consumption.

    Science.gov (United States)

    Iqbal, Shahed; Blumenthal, Wendy; Kennedy, Chinaro; Yip, Fuyuen Y; Pickard, Stephen; Flanders, W Dana; Loringer, Kelly; Kruger, Kirby; Caldwell, Kathleen L; Jean Brown, Mary

    2009-11-01

    Wild game hunting is a popular activity in many regions of the United States. Recently, the presence of lead fragments in wild game meat, presumably from the bullets or shot used for hunting, has raised concerns about health risks from meat consumption. This study examined the association between blood lead levels (PbB) and wild game consumption. We recruited 742 participants, aged 2-92 years, from six North Dakota cities. Blood lead samples were collected from 736 persons. Information on socio-demographic background, housing, lead exposure source, and types of wild game consumption (i.e., venison, other game such as moose, birds) was also collected. Generalized estimating equations (GEE) were used to determine the association between PbB and wild game consumption. Most participants reported consuming wild game (80.8%) obtained from hunting (98.8%). The geometric mean PbB were 1.27 and 0.84 microg/dl among persons who did and did not consume wild game, respectively. After adjusting for potential confounders, persons who consumed wild game had 0.30 microg/dl (95% confidence interval: 0.16-0.44 microg/dl) higher PbB than persons who did not. For all game types, recent (game consumption was associated with higher PbB. PbB was also higher among those who consumed a larger serving size (> or = 2 oz vs. game' consumption only. Participants who consumed wild game had higher PbB than those who did not consume wild game. Careful review of butchering practices and monitoring of meat-packing processes may decrease lead exposure from wild game consumption.

  6. Using Wild Olives in Breeding Programs: Implications on Oil Quality Composition

    Directory of Open Access Journals (Sweden)

    Lorenzo León

    2018-02-01

    Full Text Available A wide genetic diversity has been reported for wild olives, which could be particularly interesting for the introgression of some agronomic traits and resistance to biotic and abiotic stresses in breeding programs. However, the introgression of some beneficial wild traits may be paralleled by negative effects on some other important agronomic and quality traits. From the quality point of view, virgin olive oil (VOO from olive cultivars is highly appreciated for its fatty acid composition (high monounsaturated oleic acid content and the presence of several minor components. However, the composition of VOO from wild origin and its comparison with VOO from olive cultivars has been scarcely studied. In this work, the variability for fruit characters (fruit weight and oil content, OC, fatty acid composition, and minor quality components (squalene, sterols and tocopherols content and composition was studied in a set of plant materials involving three different origins: wild genotypes (n = 32, cultivars (n = 62 and genotypes belonging to cultivar × wild progenies (n = 62. As expected, values for fruit size and OC in wild olives were lower than those obtained in cultivated materials, with intermediate values for cultivar × wild progenies. Wild olives showed a remarkably higher C16:0 percentage and tocopherol content in comparison to the cultivars. Contrarily, lower C18:1 percentage, squalene and sterol content were found in the wild genotypes, while no clear differences were found among the different plant materials regarding composition of the tocopherol and phytosterol fractions. Some common highly significant correlations among components of the same chemical family were found in all groups of plant materials. However, some other correlations were specific for one of the groups. The results of the study suggested that the use of wild germplasm in olive breeding programs will not have a negative impact on fatty acid composition, tocopherol content

  7. Wild vegetable mixes sold in the markets of Dalmatia (southern Croatia).

    Science.gov (United States)

    Łuczaj, Łukasz; Zovkokončić, Marijana; Miličević, Tihomir; Dolina, Katija; Pandža, Marija

    2013-01-03

    Dalmatia is an interesting place to study the use of wild greens as it lies at the intersection of influence of Slavs, who do not usually use many species of wild greens, and Mediterranean culinary culture, where the use of multiple wild greens is common. The aim of the study was to document the mixtures of wild green vegetables which are sold in all the vegetable markets of Dalmatia. All vendors (68) in all 11 major markets of the Dalmatian coast were interviewed. The piles of wild vegetables they sold were searched and herbarium specimens taken from them. The mean number of species in the mix was 5.7. The most commonly sold wild plants are: Sonchus oleraceus L., Allium ampeloprasum L., Foeniculum vulgare Mill., Urospermum picroides F.W.Schmidt, Papaver rhoeas L., Daucus carota L., Taraxacum sp., Picris echioides L., Silene latifolia Poir. and Crepis spp. Also the cultivated beet (Beta vulgaris L.) and a few cultivated Brassicaceae varieties are frequent components. Wild vegetables from the mix are usually boiled for 20-30 minutes and dressed with olive oil and salt. Altogether at least 37 wild taxa and 13 cultivated taxa were recorded.Apart from the mixes, Asparagus acutifolius L. and Tamus communis L. shoots are sold in separate bunches (they are usually eaten with eggs), as well as some Asteraceae species, the latter are eaten raw or briefly boiled. The rich tradition of eating many wild greens may result both from strong Venetian and Greek influences and the necessity of using all food resources available in the barren, infertile land in the past. Although the number of wild-collected green vegetables is impressive we hypothesize that it may have decreased over the years, and that further in-depth local ethnobotanical studies are needed in Dalmatia to record the disappearing knowledge of edible plants.

  8. Effect of hunting awareness on wild game meat purchase behavior

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    Maria Elena Marescotti

    2018-06-01

    Full Text Available Although wild game meat constitutes a sustainable and healthy alternative to conventional meat and hunting contributes to the control of game populations, international studies on consumer attitudes towards this type of meat are still limited and no previous research has been focused on the Italian population. For the development of successful marketing strategies and/or public policy intervention, the knowledge of consumers’ purchase behavior is a key factor. Among all the determinants that can influence the behavior of consumers of hunted wild game meat (i.e. animal welfare, sustainability, ecological food choice, product safety, nutritional quality, the consumers’ awareness of hunting activity and their perceptions of wild game meat assume a crucial role. Accordingly, in this paper an online survey on a sample of 741 Italian meat consumers has been conducted to investigate the relationship between consumers’ purchase behavior and their awareness of hunted game meat and hunting practices (chi-square test, F-test. Statistically significant differences were found among segments of consumers with different levels of wild game meat consumption frequency. The analysis shows that, as expected, the highest consumption level of wild game meat relates to the highest level of general awareness of wild game meat and hunting practices. Our findings are in line with previous literature, that links positive behaviors of consumers towards wild game meat and hunting to familiarity and experience with hunting and hunters. Nonetheless, the present study provides a deeper understanding of the Italian consumers’ attitudes and perceptions of wild game meat and could suggests policy guidelines for the development of future targeted marketing strategies.

  9. Population genetics and disease ecology of European wild boar

    NARCIS (Netherlands)

    Goedbloed, D.J.

    2013-01-01

    Welke factoren beïnvloeden de frequentie van de ziekten in wilde populaties? Het promotieonderzoek van Daniel Goedbloed beoordeelde de invloed van demografische, genetische en omgevingsfactoren op de frequentie van twee infectieziekten in Noordwest-Europese wilde zwijnen populaties.

  10. First TBEV serological screening in Flemish wild boar

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    Sophie Roelandt

    2016-04-01

    Full Text Available In the frame of a Flemish wildlife surveillance in 2013, a serological screening was performed on sera from wild boar (Sus scrofa; n=238 in order to detect tick-borne encephalitis virus (TBEV-specific antibodies. Neutralising antibodies were titrated with a seroneutralisation test (SNT, using two cut-off titres (1/10–1/15. Seven wild boars were found TBEV-seropositive and showed moderate (>1/15 to high (>1/125 SNT-titres; three individuals had borderline results (1/10–1/15. This study demonstrated the presence of TBEV-specific antibodies in wild boar and highlighted potential TBEV-foci in Flanders. Additional surveillance including direct virus testing is now recommended.

  11. [Wild animals and law and ethics in France].

    Science.gov (United States)

    Nouët, Jean-Claude

    2013-01-01

    Legal systems applying to wild animals are very different depending on whether the animals are in captivity or under human control, or whether they are in the wild. Animals in captivity, like domesticated animals, are covered by protective measures for the welfare of the individual animal, but wild animals are not considered as individuals but only as members of a species, their numbers being controlled by humans and determined by human interests. In the light of contemporary scientific knowledge, such legal approaches are now inappropriate and can no longer be accepted for ethical reasons. The legal systems need to develop and must include a definition of the animal as an individual and as a sentient being.

  12. Diseases of wild rice

    Science.gov (United States)

    Diseases are much more pronounced in cultivated wild rice than in natural stands, most likely due to the narrower genetic base of the populations, plant stress due to high planting density and floodwater removal prior to harvest, and high relative humidity in the plant canopy. Yield losses occur as ...

  13. Gene flow and genetic diversity in cultivated and wild cacao (Theobroma cacao) in Bolivia.

    Science.gov (United States)

    Chumacero de Schawe, Claudia; Durka, Walter; Tscharntke, Teja; Hensen, Isabell; Kessler, Michael

    2013-11-01

    The role of pollen flow within and between cultivated and wild tropical crop species is little known. To study the pollen flow of cacao, we estimated the degree of self-pollination and pollen dispersal distances as well as gene flow between wild and cultivated cacao (Theobroma cacao L.). We studied pollen flow and genetic diversity of cultivated and wild cacao populations by genotyping 143 wild and 86 cultivated mature plants and 374 seedlings raised from 19 wild and 25 cultivated trees at nine microsatellite loci. A principal component analysis distinguished wild and cultivated cacao trees, supporting the notion that Bolivia harbors truly wild cacao populations. Cultivated cacao had a higher level of genetic diversity than wild cacao, presumably reflecting the varied origin of cultivated plants. Both cacao types had high outcrossing rates, but the paternity analysis revealed 7-14% self-pollination in wild and cultivated cacao. Despite the tiny size of the pollinators, pollen was transported distances up to 3 km; wild cacao showed longer distances (mean = 922 m) than cultivated cacao (826 m). Our data revealed that 16-20% of pollination events occurred between cultivated and wild populations. We found evidence of self-pollination in both wild and cultivated cacao. Pollination distances are larger than those typically reported in tropical understory tree species. The relatively high pollen exchange from cultivated to wild cacao compromises genetic identity of wild populations, calling for the protection of extensive natural forest tracts to protect wild cacao in Bolivia.

  14. Farmer–African wild dog (Lycaon pictus relations in the eastern Kalahari region of Botswana

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    Valli-Laurente Fraser-Celin

    2017-05-01

    Full Text Available African wild dogs (Lycaon pictus are the most endangered large carnivores in southern Africa. Direct and indirect persecution by farmers causes significant conservation challenges. Farmer– wild dog conflict in Botswana commonly occurs as a result of cattle and stocked game depredation by wild dogs, affecting farmer livelihood and causing economic and emotional distress. Although wild dogs predate livestock at lower levels than other carnivores, they continue to be killed both indiscriminately and in retaliation for incidents of depredation. Investigating farmer–wild dog conflict is a necessary step towards establishing appropriate conflict mitigation strategies. Eighty livestock and game farmers were interviewed in order to examine farmers’ value of, perceptions of and experiences with wild dogs as well as their insights on wild dog impacts and conservation in the eastern Kalahari region of Botswana. Interviews were semi-structured and used open-ended questions to capture complexities surrounding farmer–wild dog relations. This research contributes baseline data on wild dogs in understudied tribal land and commercial livestock and game farms in eastern Kalahari. It confirms the presence of wild dogs, livestock and stocked game depredation by wild dogs and negative perspectives amongst farmers towards wild dogs and their conservation. Mean losses were 0.85 livestock per subsistence farmer, 1.25 livestock per commercial livestock farmer, while game farmers lost 95.88 game animals per farmer during January 2012 through June 2013. Proportionally, more subsistence farmers than commercial livestock farmers and game farmers held negative perspectives of wild dogs (χ ² = 9.63, df = 2, p < 0.05. Farmer type, education level, socioeconomic status and land tenure, as well as positive wild dog characteristics should be considered when planning and operationalising conflict mitigation strategies. As such, conservation approaches should focus on

  15. Legal regulation of treatment of wild animals

    OpenAIRE

    Kolečkářová, Eliška

    2014-01-01

    The diploma thesis deals with the legal regulation of the treatment with wild animals. It compares different terms used in legal regulation of protection of animals. It specified differences between concept of an animal in private law and public law. The diploma thesis is focused on possibilities of gaining ownership to the wild animals, proving origin of animals bred in human care. It concerns with legal regulation of treatment with handicap animals. The diploma thesis analyzes preparation a...

  16. Ombre di ombre. Wilde cita Balzac II

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    Susi Pietri

    2010-12-01

    Full Text Available Oscar Wilde, “reader of Balzac” in Balzac in English and in The Decay of Lying, as well as in other essays and imagined conversations, tests an especially transgressive practice of quotation, rewriting and rereading both Balzac’s self-readings and the readings of Balzac made by other writers, such as Charles Baudelaire, Théophile Gautier and Algernon Charles Swinburne. In this way, Oscar Wilde transforms the manipulation of quotations into a new aesthetic invention. Indeed, by inserting themes and characters taken from the Comédie humaine in his own essays and dialogues, Wilde follows a complex strategy to take possession of Balzac’s inheritance and explores the performative power of the “mask”, the systematic use of critical paradoxes, the poetics of “plagiarism” and of “living plagiarism” as “reverse quotation” of Art by Life and vice versa.

  17. Microflora diversity on the phyloplane of wild Okra ( Corchorus ...

    African Journals Online (AJOL)

    Bacterial genera isolated included; Staphylococcus, Bacillus, Micrococcus, Serratia and Proteus. Rhodotorula, Mucor, Trichoderma, Aspergillus, Penicillium, and Helminthosporium were the genera of fungi isolated. Further studies could help to elucidate major players in wild okra phylloplane ecology. Keywords: Wild Okra ...

  18. Molecular phylogeny of Cyclophyllidea (Cestoda: Eucestoda): an in-silico analysis based on mtCOI gene.

    Science.gov (United States)

    Sharma, Sunil; Lyngdoh, Damanbha; Roy, Bishnupada; Tandon, Veena

    2016-09-01

    Order Cyclophyllidea (of cestode platyhelminths) has a rich diversity of parasites and includes many families and species that are known to cause serious medical condition in humans and domestic and wild animals. Despite various attempts to resolve phylogenetic relationships at the inter-family level, uncertainty remains. In order to add resolution to the existing phylogeny of the order, we generated partial mtCO1 sequences for some commonly occurring cyclophyllidean cestodes and combined them with available sequences from GenBank. Phylogeny was inferred taking a total 83 representative species spanning 8 families using Bayesian analysis. The phylogenetic tree revealed Dilepididae as the most basal taxon and showed early divergence in the phylogenetic tree. Paruterinidae, Taeniidae and Anoplocephalidae showed non-monophyletic assemblage; our result suggests that the family Paruterinidae may represent a polyphyletic group. The diverse family Taeniidae appeared in two separate clades; while one of them included all the members of the genus Echinococcus and also Versteria, the representatives of the genera Taenia and Hydatigera clubbed in the other clade. A close affinity of Dipylidiidae with Taenia and Hydatigera was seen, whereas existence of a close relationship between Mesocestoididae and Echinococcus (of Taeniidae) is also demonstrated. The crown group comprised the families Anoplocephalidae, Davaineidae, Hymenolepididae and Mesocestoididae, and also all species of the genus Echinococcus and Versteria mustelae; monophyly of these families (excepting Anolplocephalidae) and the genus Echinococcus as well as its sister-taxon relation with V. mustelae is also confirmed. Furthermore, non-monophyly of Anoplocephalidae is suggested to be correlated with divergence in the host selection.

  19. A review of ecosystem service benefits from wild bees across social contexts.

    Science.gov (United States)

    Matias, Denise Margaret S; Leventon, Julia; Rau, Anna-Lena; Borgemeister, Christian; von Wehrden, Henrik

    2017-05-01

    In order to understand the role of wild bees in both social and ecological systems, we conducted a quantitative and qualitative review of publications dealing with wild bees and the benefits they provide in social contexts. We classified publications according to several attributes such as services and benefits derived from wild bees, types of bee-human interactions, recipients of direct benefits, social contexts where wild bees are found, and sources of changes to the bee-human system. We found that most of the services and benefits from wild bees are related to food, medicine, and pollination. We also found that wild bees directly provide benefits to communities to a greater extent than individuals. In the social contexts where they are found, wild bees occupy a central role. Several drivers of change affect bee-human systems, ranging from environmental to political drivers. These are the areas where we recommend making interventions for conserving the bee-human system.

  20. Antibody Prevalence to Influenza Type A in Wild Boar of Northern Ukraine.

    Science.gov (United States)

    Kovalenko, Ganna; Molozhanova, Alona; Halka, Ihor; Nychyk, Serhiy

    2017-12-01

    A preliminary serological survey was carried out to assess the likelihood of influenza A (IA) infection in wild boar and begin to characterize the role of wild boar in the epidemiology of the IA virus (IAV). Sera collected from 120 wild boar that were hunted in 2014 were tested. To detect antibodies to IA, a blocking the enzyme-linked immunosorbent assay (ELISA) was used. Thirty boar were collected from each of four oblasts in the north central and northwestern regions of Ukraine. Antibodies against IAV were detected in 27 samples (22.5%; 95% confidence interval 16.0-30.8) and in at least some of the wild boar from all of the four oblasts. This preliminary survey of IA antibodies in wild boar populations of northern Ukraine indicates a substantial frequency of exposure to IAV throughout the region. Infection of wild boar populations could provide an alternative or additional route for spillover from wild populations to domestic animals and humans.

  1. Fungi pathogenic on wild radish (Raphanus raphanistrum L. in northern Tunisia

    Directory of Open Access Journals (Sweden)

    N. Djebali

    2009-09-01

    Full Text Available The distribution and life cycle of wild radish (Raphanus raphanistrum L. and a survey of the pathogens of this plant are reported for the northern regions of Tunisia. Wild radish is a common weed of cereal crops and legumes. It germinates in early autumn (October, develops a rosette stage in November to December after which stem growth, fl owering and pod production occur through to May, with pod maturity completed in June. Fungus isolation from the foliar tissues exhibiting disease symptoms showed that wild radish was infected with the fungi Albugo candida, Alternaria spp. including A. brassicicola, and A. raphani, Erysiphe cruciferarum, Stemphylium herbarum, Peronospora parasitica and Phoma lingam. Ascochyta spp., Cercospora armoraciae, Cladosporium cladosporioides and Colletotrichum higginsianum are here reported from wild radish for the first time. Inoculation tests of pathogens on wild radish plants showed that the most injurious fungi were Alternaria raphani and Phoma lingam. The remaining pathogens were weakly to moderately aggressive on this weed. To access the pathogenic effect of fungi spontaneously infecting natural populations of wild radish, the weed was grown in a field experiment with and without the broad-spectrum systemic fungicide Carbendazim. Results showed a statistically significant two-fold decrease in the number and weight of seed pods in the non-treated plants, indicating that the reproductive potential of wild radish was naturally reduced by fungal infection. Foliar pathogenic fungi have a potential in the integrated weed management of wild radish, this role merits further investigations.

  2. Identification of Potential Wild Herbal as parts of Landscape Elements

    Science.gov (United States)

    Sulistyantara, Bambang; Mentari, Nio

    2017-10-01

    Many landscape plants can grow on their own without cultivated by humans. They are type of plants that can be found anywhere, so they can be categorized as wild plants. The economic value of wild plants are easy to obtain and their maintenance costs are low. Because wild plants not widely known even a just a few of people that aware of their existence, it is necessary to do a study to learn the potential of the wild plants to be used as an element of landscape. This research aims to identify the species that have potential to be used in landscape design, to describe the benefits of the their implementation as a landscape element, and to recommend the wild plants that have functional value and visual. This research used a scoring method based on the functional and visual criteria, and questionnaires were conducted to 50 students of Landscape Architecture IPB who have completed Landscape Plants courses. Based on the research, there are 150 species of wild plants that found in the study site, and 60 of them are recommended as landscape elements. Then all of the species were arranged as a recommendations book so they can be used as alternative landscape plants.

  3. OSCAR WILDE OU A IDENTIDADE POÉTICA ENCARCERADA

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    Latuf Isais Mucci

    2011-01-01

    Full Text Available This article aims to expose the dandy soul of Oscar Wilde. His life proves extravagance events, liberty claims, and homoafects tolerance. The Picture of Dorian Gray's author even suffered a criminal process in reason of his relation with Lord Alfred Bruce Douglas, called Bosie, despite Wilde being married. Other biographical issues can demonstrate the fantastic experience in the flamboyant life.

  4. Aerobic salivary bacteria in wild and captive Komodo dragons.

    Science.gov (United States)

    Montgomery, Joel M; Gillespie, Don; Sastrawan, Putra; Fredeking, Terry M; Stewart, George L

    2002-07-01

    During the months of November 1996, August 1997, and March 1998, saliva and plasma samples were collected for isolation of aerobic bacteria from 26 wild and 13 captive Komodo dragons (Varanus komodoensis). Twenty-eight Gram-negative and 29 Gram-positive species of bacteria were isolated from the saliva of the 39 Komodo dragons. A greater number of wild than captive dragons were positive for both Gram-negative and Gram-positive bacteria. The average number of bacterial species within the saliva of wild dragons was 46% greater than for captive dragons. While Escherichia coli was the most common bacterium isolated from the saliva of wild dragons, this species was not present in captive dragons. The most common bacteria isolated from the saliva of captive dragons were Staphylococcus capitis and Staphylococcus capitis and Staphylococcus caseolyticus, neither of which were found in wild dragons. High mortality was seen among mice injected with saliva from wild dragons and the only bacterium isolated from the blood of dying mice was Pasteurella multocida. A competitive inhibition enzyme-linked immunosorbent assay revealed the presence of anti-Pasteurella antibody in the plasma of Komodo dragons. Four species of bacteria isolated from dragon saliva showed resistance to one or more of 16 antimicrobics tested. The wide variety of bacteria demonstrated in the saliva of the Komodo dragon in this study, at least one species of which was highly lethal in mice and 54 species of which are known pathogens, support the observation that wounds inflicted by this animal are often associated with sepsis and subsequent bacteremia in prey animals.

  5. Facultative parthenogenesis discovered in wild vertebrates.

    Science.gov (United States)

    Booth, Warren; Smith, Charles F; Eskridge, Pamela H; Hoss, Shannon K; Mendelson, Joseph R; Schuett, Gordon W

    2012-12-23

    Facultative parthenogenesis (FP)-asexual reproduction by bisexual species-has been documented in a variety of multi-cellular organisms but only recently in snakes, varanid lizards, birds and sharks. Unlike the approximately 80 taxa of unisexual reptiles, amphibians and fishes that exist in nature, FP has yet to be documented in the wild. Based on captive documentation, it appears that FP is widespread in squamate reptiles (snakes, lizards and amphisbaenians), and its occurrence in nature seems inevitable, yet the task of detecting FP in wild individuals has been deemed formidable. Here we show, using microsatellite DNA genotyping and litter characteristics, the first cases of FP in wild-collected pregnant females and their offspring of two closely related species of North American pitviper snakes-the copperhead (Agkistrodon contortrix) and cottonmouth (Agkistrodon piscivorus). Our findings support the view that non-hybrid origins of parthenogenesis, such as FP, are more common in squamates than previously thought. With this confirmation, FP can no longer be viewed as a rare curiosity outside the mainstream of vertebrate evolution. Future research on FP in squamate reptiles related to proximate control of induction, reproductive competence of parthenogens and population genetics modelling is warranted.

  6. CHARACTERIZATION OF WILD PIG VEHICLE COLLISIONS

    Energy Technology Data Exchange (ETDEWEB)

    Mayer, J; Paul E. Johns, P

    2007-05-23

    Wild pig (Sus scrofa) collisions with vehicles are known to occur in the United States, but only minimal information describing these accidents has been reported. In an effort to better characterize these accidents, data were collected from 179 wild pig-vehicle collisions from a location in west central South Carolina. Data included accident parameters pertaining to the animals involved, time, location, and human impacts. The age structure of the animals involved was significantly older than that found in the population. Most collisions involved single animals; however, up to seven animals were involved in individual accidents. As the number of animals per collision increased, the age and body mass of the individuals involved decreased. The percentage of males was significantly higher in the single-animal accidents. Annual attrition due to vehicle collisions averaged 0.8 percent of the population. Wild pig-vehicle collisions occurred year-round and throughout the 24-hour daily time period. Most accidents were at night. The presence of lateral barriers was significantly more frequent at the collision locations. Human injuries were infrequent but potentially serious. The mean vehicle damage estimate was $1,173.

  7. Wild vegetable mixes sold in the markets of Dalmatia (southern Croatia

    Directory of Open Access Journals (Sweden)

    Łuczaj Łukasz

    2013-01-01

    Full Text Available Abstract Background Dalmatia is an interesting place to study the use of wild greens as it lies at the intersection of influence of Slavs, who do not usually use many species of wild greens, and Mediterranean culinary culture, where the use of multiple wild greens is common. The aim of the study was to document the mixtures of wild green vegetables which are sold in all the vegetable markets of Dalmatia. Methods All vendors (68 in all 11 major markets of the Dalmatian coast were interviewed. The piles of wild vegetables they sold were searched and herbarium specimens taken from them. Results The mean number of species in the mix was 5.7. The most commonly sold wild plants are: Sonchus oleraceus L., Allium ampeloprasum L., Foeniculum vulgare Mill., Urospermum picroides F.W.Schmidt, Papaver rhoeas L., Daucus carota L., Taraxacum sp., Picris echioides L., Silene latifolia Poir. and Crepis spp. Also the cultivated beet (Beta vulgaris L. and a few cultivated Brassicaceae varieties are frequent components. Wild vegetables from the mix are usually boiled for 20–30 minutes and dressed with olive oil and salt. Altogether at least 37 wild taxa and 13 cultivated taxa were recorded. Apart from the mixes, Asparagus acutifolius L. and Tamus communis L. shoots are sold in separate bunches (they are usually eaten with eggs, as well as some Asteraceae species, the latter are eaten raw or briefly boiled. Conclusions The rich tradition of eating many wild greens may result both from strong Venetian and Greek influences and the necessity of using all food resources available in the barren, infertile land in the past. Although the number of wild-collected green vegetables is impressive we hypothesize that it may have decreased over the years, and that further in-depth local ethnobotanical studies are needed in Dalmatia to record the disappearing knowledge of edible plants.

  8. Movements of wild pigs in Louisiana and Mississippi, 2011-13

    Science.gov (United States)

    Hartley, Stephen B.; Goatcher, Buddy L.; Sapkota, Sijan

    2015-01-01

    The prolific breeding capability, behavioral adaptation, and adverse environmental impacts of invasive wild pigs (Sus scrofa) have increased efforts towards managing their populations and understanding their movements. Currently, little is known about wild pig populations and movements in Louisiana and Mississippi. From 2011 to 2013, the U.S. Geological Survey investigated spatial and temporal movements of wild pigs in both marsh and nonmarsh physiographic regions. Twenty-one Global Positioning System satellite telemetry tracking collars were installed on adult wild pigs captured with trained dogs and released. Coordinates of their locations were recorded hourly. We collected 16,674 hourly data points including date, time, air temperature, and position during a 3-year study. Solar and lunar attributes, such as sun and moon phases and azimuth angles, were not related significantly to the movements among wild pigs. Movements were significantly correlated negatively with air temperature. Differences in movements between seasons and years were observed. On average, movements of boars were significantly greater than those of sows. Average home range, determined by using a minimum convex polygon as a proxy, was 911 hectares for boars, whereas average home range for sows was 116 hectares. Wild pigs in marsh habitat traveled lesser distances relative to those from more arid, nonmarsh habitats. Overall, results of this study indicate that wild pigs in Louisiana and Mississippi have small home ranges. These small home ranges suggest that natural movements have not been a major factor in the recent broad-scale range expansion observed in this species in the United States.

  9. Eating from the wild: diversity of wild edible plants used by Tibetans in Shangri-la region, Yunnan, China.

    Science.gov (United States)

    Ju, Yan; Zhuo, Jingxian; Liu, Bo; Long, Chunlin

    2013-04-19

    Locally harvested wild edible plants (WEPs) provide food as well as cash income for indigenous people and are of great importance in ensuring global food security. Some also play a significant role in maintaining the productivity and stability of traditional agro-ecosystems. Shangri-la region of Yunnan Province, SW China, is regarded as a biodiversity hotspot. People living there have accumulated traditional knowledge about plants. However, with economic development, WEPs are threatened and the associated traditional knowledge is in danger of being lost. Therefore, ethnobotanical surveys were conducted throughout this area to investigate and document the wild edible plants traditionally used by local Tibetan people. Twenty-nine villages were selected to carry out the field investigations. Information was collected using direct observation, semi-structured interviews, individual discussions, key informant interviews, focus group discussions, questionnaires and participatory rural appraisal (PRA). Information about 168 wild edible plant species in 116 genera of 62 families was recorded and specimens were collected. Most species were edible greens (80 species) or fruits (78). These WEPs are sources for local people, especially those living in remote rural areas, to obtain mineral elements and vitamins. More than half of the species (70%) have multiple use(s) besides food value. Some are crop wild relatives that could be used for crop improvement. Several also have potential values for further commercial exploitation. However, the utilization of WEPs and related knowledge are eroding rapidly, especially in the areas with convenient transportation and booming tourism. Wild food plants species are abundant and diverse in Shangri-la region. They provide food and nutrients to local people and could also be a source of cash income. However, both WEPs and their associated indigenous knowledge are facing various threats. Thus, conservation and sustainable utilization of these

  10. Mitochondrial DNA control region variability in wild boars from west Balkans

    Directory of Open Access Journals (Sweden)

    Đan Mihajla

    2013-01-01

    Full Text Available The wild boar (Sus scrofa is one of most abundant game species in hunting areas of Balkan region. The large fraction of pre-glacial genetic diversity in wild boar populations from the Balkans was addressed due to high proportion of unique mtDNA haplotypes found in Greece, indicating Balkan as main refugial area for wild boars. The aim of the present study is to characterize mitochondrial DNA control region variability in wild boars from different areas in the West Balkan region, in order to evaluate level of genetic variability, to detect unique haplotypes and to infer possible structuring. The total number of 163 individuals from different sampling localities were included in the study. A fragment of the mtDNA control region was amplified and sequenced by standard procedures. Population genetic analyses were performed using several computer packages: BioEdit, ARLEQUIN 3.5.1.2., Network 4.6.0.0 and MEGA5. Eleven different haplotypes were identified and haplotype diversity was 0.676, nucleotide diversity 0.0026, and the average number of nucleotide differences (k 1.169. The mismatch distribution and neutrality tests indicated the expansion of the all populations. It is shown that high level of genetic diversity is present in the wild boars from the West Balkan region and we have managed to detect regional unique haplotypes in high frequency. Genetic diversity differences have been found in regional wild boar groups, clustering them in two main clusters, but further speculations on the reasons for the observed clustering are prevented due to restricted informativness of the single locus marker. Obtained knowledge of genetic variation in the wild boar may be relevant for improving knowledge of the phylogeny and phylogeography of the wild boars, but as well as for hunting societies and responsible authorities for the effective control of wild boar populations.

  11. Connectivity of the Asiatic wild ass population in the Mongolian Gobi.

    Science.gov (United States)

    Kaczensky, Petra; Kuehn, Ralph; Lhagvasuren, Badamjav; Pietsch, Stephanie; Yang, Weikang; Walzer, Chris

    2011-02-01

    Long-distance migrations of wildlife have been identified as important biological phenomena, but their conservation remains a major challenge. The Mongolian Gobi is one of the last refuges for the Asiatic wild ass (Equus hemionus) and other threatened migratory mammals. Using historic and current distribution ranges, population genetics, and telemetry data we assessed the connectivity of the wild ass population in the context of natural and anthropogenic landscape features and the existing network of protected areas. In the Mongolian Gobi mean biomass production is highly correlated with human and livestock density and seems to predict wild ass occurrence at the upper level. The current wild ass distribution range largely falls into areas below the 250 gC/m(2)/year productivity isoline, suggesting that under the present land use more productive areas have become unavailable for wild asses. Population genetics results identified two subpopulations and delineated a genetic boundary between the Dzungarian and Transaltai Gobi for which the most likely explanation are the mountain ranges separating the two areas. Home ranges and locations of 19 radiomarked wild asses support the assumed restricting effects of more productive habitats and mountain ranges and additionally point towards a barrier effect of fences. Furthermore, telemetry data shows that in the Dzungarian and Transaltai Gobi individual wild ass rarely ventured outside of the protected areas, whereas in the southeast Gobi asses only spend a small fraction of their time within the protected area network. Conserving the continuity of the wild ass population will need a landscape level approach, also including multi-use landscapes outside of protected areas, particularly in the southeast Gobi. In the southwest Gobi, allowing for openings in the border fence to China and managing the border area as an ecological corridor would connect three large protected areas together covering over 70,000 km(2) of wild ass

  12. Mycobacterium spp. in wild game in Slovenia.

    Science.gov (United States)

    Pate, Mateja; Zajc, Urška; Kušar, Darja; Žele, Diana; Vengušt, Gorazd; Pirš, Tina; Ocepek, Matjaž

    2016-02-01

    Wildlife species are an important reservoir of mycobacterial infections that may jeopardise efforts to control and eradicate bovine tuberculosis (bTB), caused by Mycobacterium bovis. Slovenia is officially free of bTB, but no data on the presence of mycobacteria in wild animals has been reported. In this study, samples of liver and lymph nodes were examined from 306 apparently healthy free-range wild animals of 13 species in Slovenia belonging to the families Cervidae, Suidae, Canidae, Mustelidae and Bovidae. Mycobacteria were isolated from 36/306 (11.8%) animals (red deer, roe deer, fallow deer, wild boar and jackal) and identified by PCR, commercial diagnostic kits and sequencing. Non-tuberculous mycobacteria identified in five species were Mycobacterium peregrinum, M. avium subsp. hominissuis, M. intracellulare, M. confluentis, M. fortuitum, M. terrae, M. avium subsp. avium, M. celatum, M. engbaekii, M. neoaurum, M. nonchromogenicum and M. vaccae. Copyright © 2015 Elsevier Ltd. All rights reserved.

  13. Columbia River wildlife mitigation habitat evaluation procedures report: Scotch Creek Wildlife Area, Berg Brothers, and Douglas County pygmy rabbit projects

    International Nuclear Information System (INIS)

    Ashley, P.R.; Ratassepp, J.; Berger, M.; Judd, S.L.

    1997-01-01

    This Habitat Evaluation Procedure study was conducted to determine baseline habitat units (HUs) on the Scotch Creek, Mineral Hill, Pogue Mountain, Chesaw and Tunk Valley Habitat Areas (collectively known as the Scotch Creek Wildlife Area) in Okanogan County, Sagebrush Flat and the Dormaler property in Douglas County, and the Berg Brothers ranch located in Okanogan County within the Colville Reservation. A HEP team comprised of individuals from the Washington Department of Fish and Wildlife, the Confederated Tribes of the Colville Reservation, and the Natural Resources Conservation Service (Appendix A) conducted baseline habitat surveys using the following HEP evaluation species: mule deer (Odocoileus hemionus), sharp-tailed grouse (Tympanuchus phasianellus), pygmy rabbit (Brachylagus idahoensis), white-tailed deer (Odocoileus virginiana), mink (Mustela vison), Canada goose (Branta canadensis), downy woodpecker (Picoides pubescens), Lewis woodpecker (Melanerpes lewis), and Yellow warbler (Dendroica petechia). Results of the HEP analysis are listed below. General ratings (poor, marginal, fair, etc.,) are described in Appendix B. Mule deer habitat was marginal lacking diversity and quantify of suitable browse species. Sharp-tailed grouse habitat was marginal lacking residual nesting cover and suitable winter habitat Pygmy rabbit habitat was in fair condition except for the Dormaier property which was rated marginal due to excessive shrub canopy closure at some sites. This report is an analysis of baseline habitat conditions on mitigation project lands and provides estimated habitat units for mitigation crediting purposes. In addition, information from this document could be used by wildlife habitat managers to develop management strategies for specific project sites

  14. Columbia River Wildlife Mitigation Habitat Evaluation Procedures Report / Scotch Creek Wildlife Area, Berg Brothers, and Douglas County Pygmy Rabbit Projects.

    Energy Technology Data Exchange (ETDEWEB)

    Ashley, Paul R.

    1997-01-01

    This Habitat Evaluation Procedure study was conducted to determine baseline habitat units (HUs) on the Scotch Creek, Mineral Hill, Pogue Mountain, Chesaw and Tunk Valley Habitat Areas (collectively known as the Scotch Creek Wildlife Area) in Okanogan County, Sagebrush Flat and the Dormaler property in Douglas County, and the Berg Brothers ranch located in Okanogan County within the Colville Reservation. A HEP team comprised of individuals from the Washington Department of Fish and Wildlife, the Confederated Tribes of the Colville Reservation, and the Natural Resources Conservation Service (Appendix A) conducted baseline habitat surveys using the following HEP evaluation species: mule deer (Odocoileus hemionus), sharp-tailed grouse (Tympanuchus phasianellus), pygmy rabbit (Brachylagus idahoensis), white-tailed deer (Odocoileus virginiana), mink (Mustela vison), Canada goose (Branta canadensis), downy woodpecker (Picoides pubescens), Lewis woodpecker (Melanerpes lewis), and Yellow warbler (Dendroica petechia). Results of the HEP analysis are listed below. General ratings (poor, marginal, fair, etc.,) are described in Appendix B. Mule deer habitat was marginal lacking diversity and quantify of suitable browse species. Sharp-tailed grouse habitat was marginal lacking residual nesting cover and suitable winter habitat Pygmy rabbit habitat was in fair condition except for the Dormaier property which was rated marginal due to excessive shrub canopy closure at some sites. This report is an analysis of baseline habitat conditions on mitigation project lands and provides estimated habitat units for mitigation crediting purposes. In addition, information from this document could be used by wildlife habitat managers to develop management strategies for specific project sites.

  15. Radiocesium uptake mechanisms in wild and culture mushrooms

    International Nuclear Information System (INIS)

    Sugiyama, Hideo; Terada, Hiroshi; Isomura, Kimio; Tsukada, Hirofumi; Shibata, Hisashi.

    1993-01-01

    Concentrations of 137 Cs and stable Cs in wild mushrooms, cultivated mushrooms and those substrates were measured by gamma-ray spectrometry and neutron activation analysis. The average concentration of 137 Cs in 80 wild mushrooms in Japan was 87.5 Bq/kg (wet wt.), and concentration of 137 Cs in mycorrhizal mushrooms was higher than that of saprophytic mushrooms. High concentrations of 137 Cs were found in Pleurotus ostreatus (Fr.) Kummer Y-1, saprophytic mushrooms, cultivated in culture substrates containing high 137 Cs. Clear correlations with 5% level of significance were found between wild mushroom-to-substrate ratios (wet/dry) of 137 Cs concentration and those of stable Cs. Cultivated P. ostreatus-to-culture substrate ratios (wet/wet) of 137 Cs concentration were stable in the order of 10 0 when the culture substrate was containing 10 000 Bq/kg (wet wt.) of 137 Cs or 1 000 mg/kg (wet wt.) of stable Cs. The ratios of 137 Cs concentration in cultivated mushrooms were about equal to those in wild mushrooms. Higher concentration of 137 Cs in culture substrate after sampling P. ostreatus was observed at the upper layer where mycelium density was high. (author)

  16. Understanding Urban Demand for Wild Meat in Vietnam: Implications for Conservation Actions.

    Directory of Open Access Journals (Sweden)

    Rachel Shairp

    Full Text Available Vietnam is a significant consumer of wildlife, particularly wild meat, in urban restaurant settings. To meet this demand, poaching of wildlife is widespread, threatening regional and international biodiversity. Previous interventions to tackle illegal and potentially unsustainable consumption of wild meat in Vietnam have generally focused on limiting supply. While critical, they have been impeded by a lack of resources, the presence of increasingly organised criminal networks and corruption. Attention is, therefore, turning to the consumer, but a paucity of research investigating consumer demand for wild meat will impede the creation of effective consumer-centred interventions. Here we used a mixed-methods research approach comprising a hypothetical choice modelling survey and qualitative interviews to explore the drivers of wild meat consumption and consumer preferences among residents of Ho Chi Minh City, Vietnam. Our findings indicate that demand for wild meat is heterogeneous and highly context specific. Wild-sourced, rare, and expensive wild meat-types are eaten by those situated towards the top of the societal hierarchy to convey wealth and status and are commonly consumed in lucrative business contexts. Cheaper, legal and farmed substitutes for wild-sourced meats are also consumed, but typically in more casual consumption or social drinking settings. We explore the implications of our results for current conservation interventions in Vietnam that attempt to tackle illegal and potentially unsustainable trade in and consumption of wild meat and detail how our research informs future consumer-centric conservation actions.

  17. Understanding Urban Demand for Wild Meat in Vietnam: Implications for Conservation Actions

    Science.gov (United States)

    Shairp, Rachel; Veríssimo, Diogo; Fraser, Iain; Challender, Daniel; MacMillan, Douglas

    2016-01-01

    Vietnam is a significant consumer of wildlife, particularly wild meat, in urban restaurant settings. To meet this demand, poaching of wildlife is widespread, threatening regional and international biodiversity. Previous interventions to tackle illegal and potentially unsustainable consumption of wild meat in Vietnam have generally focused on limiting supply. While critical, they have been impeded by a lack of resources, the presence of increasingly organised criminal networks and corruption. Attention is, therefore, turning to the consumer, but a paucity of research investigating consumer demand for wild meat will impede the creation of effective consumer-centred interventions. Here we used a mixed-methods research approach comprising a hypothetical choice modelling survey and qualitative interviews to explore the drivers of wild meat consumption and consumer preferences among residents of Ho Chi Minh City, Vietnam. Our findings indicate that demand for wild meat is heterogeneous and highly context specific. Wild-sourced, rare, and expensive wild meat-types are eaten by those situated towards the top of the societal hierarchy to convey wealth and status and are commonly consumed in lucrative business contexts. Cheaper, legal and farmed substitutes for wild-sourced meats are also consumed, but typically in more casual consumption or social drinking settings. We explore the implications of our results for current conservation interventions in Vietnam that attempt to tackle illegal and potentially unsustainable trade in and consumption of wild meat and detail how our research informs future consumer-centric conservation actions. PMID:26752642

  18. Understanding Urban Demand for Wild Meat in Vietnam: Implications for Conservation Actions.

    Science.gov (United States)

    Shairp, Rachel; Veríssimo, Diogo; Fraser, Iain; Challender, Daniel; MacMillan, Douglas

    2016-01-01

    Vietnam is a significant consumer of wildlife, particularly wild meat, in urban restaurant settings. To meet this demand, poaching of wildlife is widespread, threatening regional and international biodiversity. Previous interventions to tackle illegal and potentially unsustainable consumption of wild meat in Vietnam have generally focused on limiting supply. While critical, they have been impeded by a lack of resources, the presence of increasingly organised criminal networks and corruption. Attention is, therefore, turning to the consumer, but a paucity of research investigating consumer demand for wild meat will impede the creation of effective consumer-centred interventions. Here we used a mixed-methods research approach comprising a hypothetical choice modelling survey and qualitative interviews to explore the drivers of wild meat consumption and consumer preferences among residents of Ho Chi Minh City, Vietnam. Our findings indicate that demand for wild meat is heterogeneous and highly context specific. Wild-sourced, rare, and expensive wild meat-types are eaten by those situated towards the top of the societal hierarchy to convey wealth and status and are commonly consumed in lucrative business contexts. Cheaper, legal and farmed substitutes for wild-sourced meats are also consumed, but typically in more casual consumption or social drinking settings. We explore the implications of our results for current conservation interventions in Vietnam that attempt to tackle illegal and potentially unsustainable trade in and consumption of wild meat and detail how our research informs future consumer-centric conservation actions.

  19. Salmonella spp. dynamics in wild blueberry, Vaccinium angustifolium Aiton

    Science.gov (United States)

    A six-year field study was conducted in the two major wild, or lowbush, blueberry growing regions in Maine, Midcoast and Downeast. This study used data from two cropping cycles (four years) to model the dynamics of Salmonella spp. prevalence in wild blueberry fields (Vaccinium angustifolium Aiton). ...

  20. Synthetic Hexaploids Derived from Wild Species Related to Sweet Potato

    OpenAIRE

    SHIOTANI, Itaru; KAWASE, Tsuneo; 塩谷, 格; 川瀬, 恒男

    1987-01-01

    The utilization of germplasm of the wild species in sweet-potato breeding has been conducted for the last three decades. Such attempts brought some remarkable achievments in improving root yield, starch content and resistance to the nematodes of sweet potato. Some wild plants in polyploid series may have many genes potentially important for further improvement of the agronomic traits. However, the genomic relationship between the wild relatives and hexaploid sweet potato (2n=6x=90) has been u...