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Sample records for watanabe rabbits fed

  1. Increased plant sterol and stanol levels in brain of Watanabe rabbits fed rapeseed oil derived plant sterol or stanol esters

    DEFF Research Database (Denmark)

    Fricke, Christiane B.; Schrøder, Malene; Poulsen, Morten

    2007-01-01

    of these components in brain tissue of homozygous and heterozygous Watanabe rabbits, an animal model for familial hypercholesterolemia. Homozygous animals received either a standard diet, RSO stanol or RSO sterol ester while heterozygous animals were additionally fed with 2 g cholesterol/kg to the respective diet...

  2. Probucol selectively increases oxidation of atherogenic lipoproteins in cholesterol-fed mice and in Watanabe heritable hyperlipidemic rabbits

    DEFF Research Database (Denmark)

    Lauridsen, S.T.; Mortensen, Alicja

    1999-01-01

    The anti-atherogenic and cholesterol-lowering drug probucol (0.5-1%) or quercetin (1%), a natural antioxidant, was given to cholesterol-fed (1.5%) mice for a period of 6 weeks and to Watanabe heritable hyperlipidemic (WHHL) rabbits for a period of 8 weeks to investigate the oxidative changes...... in plasma and lipoproteins. Oxidation was measured as the total amount of malondialdehyde (nmol MDA/g protein) by a very specific MDA-HPLC method. A large and significant increase in MDA was seen in LDL from probucol treated WHHL rabbits (1778.7 +/- 585.5 nmol/g vs. 394.4 +/- 144.5 nmol/g, P ....001) and cholesterol-fed mice (579.7 +/- 47.3 nmol/g vs. 408.1 +/- 85.8 nmol/g, P rabbits: P rabbits VLDL oxidation was determined additionally, and also revealed a large increase in the probucol group (2102...

  3. New animal model for the study of postmenopausal coronary and cerebral artery function: the Watanabe heritable hyperlipidemic rabbit fed on a diet avoiding phytoestrogens

    DEFF Research Database (Denmark)

    Dalsgaard, T; Larsen, C R; Mortensen, A

    2002-01-01

    to treatment for 16 weeks with either 17 beta-estradiol or placebo. The chow used was semi-synthetic, thereby avoiding the influence of phytoestrogens. Ring segments of cerebral and coronary arteries were mounted for isometric tension recordings in myographs. The passive and active length-tension relationships...... in the proximal coronary arteries. No changes were observed for the passive length-tension relationships. CONCLUSIONS: Long-term treatment with 17 beta-estradiol lowered the electromechanical tonus of atherosclerotic coronary arteries proximally, where the atherosclerosis is most developed. This could be one......OBJECTIVE: To evaluate the effect of estrogen replacement therapy (ERT) on the functional characteristics of coronary and cerebral arteries in a new rabbit model for postmenopausal vascular function. METHODS: Female ovariectomized Watanabe heritable hyperlipidemic (WHHL) rabbits were randomized...

  4. (-)-anipamil retards atherosclerosis in Watanabe heritable hyperlipidemic rabbits

    DEFF Research Database (Denmark)

    Hansen, B F; Mortensen, A; Hansen, J F

    1995-01-01

    Calcium antagonists have been reported to limit atherosclerosis in cholesterol fed rabbits. The purpose of this study was to examine the effect of the calcium antagonist (-)-anipamil on the spontaneous development of atherosclerosis in homozygote WHHL rabbits. From the age of 7 weeks, three groups...... differences were found in serum lipids (i.e., VLDL, IDL, LDL, HDL) in the study period among the three groups. Plasma anipamil at the end of the study was 0.23 +/- 6, and 202 +/- 19 ng/ml, respectively, in the three treatment groups. The degree of atherosclerosis in the abdominal aorta was significantly lower...... (p atherosclerosis in the abdominal aorta in WHHL rabbits....

  5. Effects of red grape skin and seed extract supplementation on atherosclerosis in Watanabe heritable hyperlipidemic rabbits

    DEFF Research Database (Denmark)

    Frederiksen, Hanne; Mortensen, Alicja; Schrøder, Malene;

    2007-01-01

    Epidemiological studies have suggested an association between consumption of red wine and other polyphenolic compounds and prevention of cardiovascular diseases. In the present study, Watanabe heritable hyperlipidemic (WHHL) rabbits were used to investigate the effects of polyphenols in a red gra...

  6. Dietary supplementation with an extract of lycopene-rich tomatoes does not reduce atherosclerosis in Watanabe Heritable Hyperlipidemic rabbits

    DEFF Research Database (Denmark)

    Frederiksen, H.; Rasmussen, S.E.; Schrøder, Malene;

    2007-01-01

    Tomatoes are rich in lycopene and other carotenoids which have shown beneficial effects on CVD in epidemiological and intervention studies. In the present study the effect of an extract of lycopene-rich tomatoes, Lyc-O-Mato (R) on atherosclerosis was studied in Watanabe Heritable Hyperlipidemic...... rabbits. The rabbits were fed a control diet, a control diet supplemented with the tomato extract or a control diet supplemented with a mixture of plant oils for 16 weeks. Lycopene was detected only in plasma of rabbits receiving tomato extract. The tomato extract had no effect on cholesterol...... and triacylglycerol levels measured in total plasma, lipoprotein fractions and on aortic atherosclerosis evaluated biochemically and by microscopy. Oxidation of lipids in unfractionated plasma also was unaffected by the intake of tomato extract. In conclusion, the tomato extract increased plasma levels of lycopene...

  7. Absorption and excretion of black currant anthocyanins in human and Watanabe Heritable Hyperlipidemic rabbits

    DEFF Research Database (Denmark)

    Nielsen, I. L.. F.; Ravn-Haren, Gitte; Dragsted, L. O.;

    2003-01-01

    Anthocyanins are thought to protect against cardiovascular diseases. Watanabe heritable hyperlipidemic (WHHL) rabbits are hypercholesterolemic and used as a model of the development of atherosclerosis. To compare the uptake and excretion of anthocyanins in humans and WHHL rabbits, single-dose black...... currant anthocyanin studies were performed. Procedures for workup and analyses of urine and plasma samples containing anthocyanins were developed with high recoveries (99 and 81%, respectively) and low limits of quantification (greater than or equal to 6.6 and greater than or equal to 1.1 nM, respectively......). The excretion and absorption of anthocyanins from black currant juice were found to be within the same order of magnitude in the two species regarding urinary excretion within the first 4 h (rabbits, 0.035%; humans, 0.072%) and t(ma)x (rabbits, similar to30 min; humans, similar to45 min). A food matrix effect...

  8. Anthocyanins increase low-density lipoprotein and plasma cholesterol and do not reduce atherosclerosis in Watanabe Heritable Hyperlipidemic rabbits

    DEFF Research Database (Denmark)

    Nielsen, I. L. F.; Rasmussen, S.E.; Mortensen, Alicja;

    2005-01-01

    Anthocyanin-rich beverages have shown beneficial effects on coronary heart disease in epidemiological and intervention studies. In the present study, we investigated the effect of black currant anthocyanins on atherosclerosis. Watanabe Heritable Hyperlipidemic rabbits (n = 61) were fed either...... a purified anthocyanin fraction front black currants, a black currant juice, probucol or control diet for 16 weeks. Purified anthocyanins significantly increased plasma cholesterol and low-density lipoprotein (LDL) cholesterol. Intake of black currant juice had no effect on total plasma cholesterol......, but lowered very-low-density lipoprotein (VLDL) cholesterol significantly. There were no significant effects of either purified anthocyanins or black currant juice on aortic cholesterol or development of atherosclerosis after 16 weeks. Probucol had no effect on plasma cholesterol but significantly lowered...

  9. Pentosan polysulfate inhibits atherosclerosis in Watanabe heritable hyperlipidemic rabbits: differential modulation of metalloproteinase-2 and -9.

    Science.gov (United States)

    Lupia, Enrico; Zheng, Feng; Grosjean, Fabrizio; Tack, Ivan; Doublier, Sophie; Elliot, Sharon J; Vlassara, Helen; Striker, Gary E

    2012-02-01

    Pentosan polysulfate (PPS), a heparinoid compound essentially devoid of anticoagulant activity, modulates cell growth and decreases inflammation. We investigated the effect of PPS on the progression of established atherosclerosis in Watanabe heritable hyperlipidemic (WHHL) rabbits. After severe atherosclerosis developed on an atherogenic diet, WHHL rabbits were treated with oral PPS or tap water for 1 month. The aortic intima-to-media ratio and macrophage infiltration were reduced, plaque collagen content was increased, and plaque fibrous caps were preserved by PPS treatment. Plasma lipid levels and post-heparin hepatic lipase activity remained unchanged. However, net collagenolytic activity in aortic extracts was decreased, and the levels of matrix metalloproteinase (MMP)-2 and tissue inhibitor of metalloproteinase (TIMP) activity were increased by PPS. Moreover, PPS treatment decreased tumor necrosis factor α (TNFα)-stimulated proinflammatory responses, in particular activation of nuclear factor-κB and p38, and activation of MMPs in macrophages. In conclusion, oral PPS treatment prevents progression of established atherosclerosis in WHHL rabbits. This effect may be partially mediated by increased MMP-2 and TIMP activities in the aortic wall and reduced TNFα-stimulated inflammation and MMP activation in macrophages. Thus, PPS may be a useful agent in inhibiting the progression of atherosclerosis.

  10. Adenosine-induced coronary flow reserve in Watanabe heritable hyperlipidemic rabbits

    Energy Technology Data Exchange (ETDEWEB)

    Shimada, Kazuhiro; Yoshida, Katsuya [Chiba Univ. (Japan). School of Medicine; Tadokoro, Hiroyuki [and others

    2000-12-01

    The Watanabe heritable hyperlipidemic (WHHL) rabbit develops coronary atherosclerosis and hypercholesterolemia because of a genetic deficiency of low-density lipoprotein receptors and is therefore a good animal model for studying the relationships of coronary atherosclerosis, hypercholesterolemia and coronary flow reserve. The aim of the present study was to assess myocardial perfusion at baseline and during adenosine infusion (0.2 mg{center_dot}kg{sup -1}{center_dot}min{sup -1}) in 8 WHHL rabbits (13.8{+-}0.5 months) with {sup 13}N-ammonia, small-animal positron emission tomography (PET) and colored microspheres. Results were compared with those from 6 age-matched Japanese white rabbits. Plaque distribution was also examined in the extramural coronary arteries. All 8 WHHL rabbits had coronary plaques, with 6 showing multiple plaques. Mean global myocardial blood flow (ml{center_dot}min{sup -1}{center_dot}g{sup -1}) did not differ significantly between control and WHHL groups both at baseline (3.67{+-}0.72 vs 4.26{+-}1.12 ml{center_dot}min{sup -1}{center_dot}g{sup -1}, p=NS) and with adenosine (7.92{+-}2.00 vs 9.27{+-}2.91 ml{center_dot}min{sup -1}{center_dot}g{sup -1}, p=NS), nor did coronary flow reserve (2.16{+-}0.37 vs 2.18{+-}0.41, p=NS). None showed evidence of regional perfusion abnormalities by visual and semiquantitative analyses of PET images. It was concluded that WHHL rabbits preserve adenosine-induced coronary flow reserve despite coronary atherosclerosis and hypercholesterolemia, suggesting that a compensatory mechanism develops in this animal model. (author)

  11. Stanol esters attenuate the aggravating effect of dietary cholesterol on atherosclerosis in homozygous Watanabe rabbits

    DEFF Research Database (Denmark)

    Schrøder, Malene; Husche, Constanze; Pilegaard, Kirsten

    2009-01-01

    Plant stanols are marketed as natural means to lower blood cholesterol in humans; hence the effect on combined familial hyperlipidemia is not known. The objective was to investigate the effect of stanol esters on blood lipids and aortic atherosclerosis in homozygous WHHL rabbits challenged...... with dietary cholesterol. A total of 36 rabbits, 6 weeks of age, with initial plasma cholesterol of 22.5 mmol/L were assigned to two treatment groups fed a standard rabbit chow with 1 g/kg cholesterol or this diet added 34 g/kg stanol ester, respectively, for 16 weeks. Plasma cholesterol was measured initially...... and at termination, also in lipoproteins. Aortic atherosclerosis was evaluated as cholesterol content and area covered by plaque. Plasma cholesterol was not significantly different between the groups at termination (35.7 mmol/L vs. 35.5 mmol/L). A significant increase in LDL was seen (13.1 mmol/L vs. 16.5 mmol...

  12. Effect of rapeseed oil derived plant sterol and stanol esters on atherosclerosis parameters in cholesterol challenged heterozygous Watanabe Heritable Hyperlipidemic rabbits

    DEFF Research Database (Denmark)

    Schrøder, Malene; Fricke, Christiane; Pilegaard, Kirsten

    2009-01-01

    Rapeseed oil (RSO) is a novel source of plant sterols, containing the unique brassicasterol in concentrations higher than allowed for plant sterol blends in food products in the European Union. Effects of RSO sterols and stanols on aortic atherosclerosis were studied in cholesterol-fed heterozygous...... Watanabe heritable hyperlipidaemic (Hh-WHHL) rabbits. Four groups (n 18 per group) received a cholesterol-added (2 g/kg) standard chow or this diet with added RSO stanol esters (17 g/kg), RSO stanol esters (34 g/kg) or RSO sterol esters (34 g/kg) for 18 weeks. Feeding RSO stanol esters increased plasma...... campestanol (P sterol esters increased concentrations of plasma campesterol (P

  13. Estrogen and phytoestrogens: Effect on eNOS expression and in vitro vasodilation in cerebral arteries in ovariectomized Watanabe heritable hyperlipidemic rabbits

    DEFF Research Database (Denmark)

    Lund, Claus Otto; Mortensen, A; Nilas, Lisbeth

    2007-01-01

    -six female ovariectomized Watanabe heritable hyperlipidemic (WHHL) rabbits were randomised to treatment with 17beta-estradiol (17beta-E(2)), SoyLife 150 or control for 16 weeks. Ring segments of basilar artery (BA) and posterior cerebral artery (PCA) were mounted in myographs for isometric tension recordings......OBJECTIVES: To evaluate the effect of estrogen replacement therapy or soy isoflavones supplement on endothelium-dependent relaxation in vitro and gene expression of endothelial nitric oxide synthase (eNOS) in cerebral arteries in a rabbit model of human hypercholesterolemia. STUDY DESIGN: Thirty...... in any of the arteries. Correspondingly, eNOS mRNA was similarly expressed in all treatment groups in both arteries. CONCLUSIONS: Improvement of cerebral endothelial function by estrogen or soy isoflavones in ovariectomized WHHL rabbits is not supported by the present data. The findings may be unique...

  14. Estrogen and phytoestrogens: Effect on eNOS expression and in vitro vasodilation in cerebral arteries in ovariectomized Watanabe heritable hyperlipidemic rabbits

    DEFF Research Database (Denmark)

    Lund, Claus O.; Mortensen, Alicja; Nilas, Lisbeth

    2007-01-01

    -six female ovariectomized Watanabe heritable hyperlipidemic (WHHL) rabbits were randomised to treatment with 17 beta-estradiol (17 beta-E-2), SoyLife 150(R) or control for 16 weeks. Ring segments of basilar artery (BA) and posterior cerebral artery (PCA) were mounted in myographs for isometric tension......Objectives: To evaluate the effect of estrogen replacement therapy or soy isoflavones supplement on endothelium-dependent relaxation in vitro and gene expression of endothelial nitric oxide synthase (eNOS) in cerebral arteries in a rabbit model of human hypercholesterolemia. Study design: Thirty......-independent response to SNP in any of the arteries. Correspondingly, eNOS mRNA was similarly expressed in all treatment groups in both arteries. Conclusions: Improvement of cerebral endothelial function by estrogen or soy isoflavones in ovariectomized WHHL rabbits is not supported by the present data. The findings may...

  15. Estrogen and phytoestrogens: Effect on eNOS expression and in vitro vasodilation in cerebral arteries in ovariectomized Watanabe heritable hyperlipidemic rabbits

    DEFF Research Database (Denmark)

    Lund, Claus Otto; Mortensen, A; Nilas, Lisbeth

    2007-01-01

    OBJECTIVES: To evaluate the effect of estrogen replacement therapy or soy isoflavones supplement on endothelium-dependent relaxation in vitro and gene expression of endothelial nitric oxide synthase (eNOS) in cerebral arteries in a rabbit model of human hypercholesterolemia. STUDY DESIGN: Thirty......-six female ovariectomized Watanabe heritable hyperlipidemic (WHHL) rabbits were randomised to treatment with 17beta-estradiol (17beta-E(2)), SoyLife 150 or control for 16 weeks. Ring segments of basilar artery (BA) and posterior cerebral artery (PCA) were mounted in myographs for isometric tension recordings...... in any of the arteries. Correspondingly, eNOS mRNA was similarly expressed in all treatment groups in both arteries. CONCLUSIONS: Improvement of cerebral endothelial function by estrogen or soy isoflavones in ovariectomized WHHL rabbits is not supported by the present data. The findings may be unique...

  16. Atherosclerosis in Watanabe heritable hyperlipidaemic rabbits. Evaluation by macroscopic, microscopic and biochemical methods and comparison of atherosclerosis variables

    DEFF Research Database (Denmark)

    Hansen, B F; Mortensen, A; Hansen, J F

    1994-01-01

    estimation of aortic atherosclerosis extent and by biochemical analysis of aortic cholesterol content. No noteworthy atherosclerosis was demonstrated within 19 months in heterozygous rabbits. In homozygous rabbits, atherosclerotic lesions were seen from the age of 4 months and progressed with age. All 19......-month-old rabbits had severe atherosclerotic disease. As much as 64% of the variation in atherosclerosis extent/severity could be explained by serum cholesterol and age. A highly significant correlation between the various methods for quantitation of atherosclerosis extent and/or severity...... was demonstrated, suggesting that quantitative microscopy, macroscopic morphometry and determination of aortic cholesterol content may be equally valid as a measure of atherosclerosis in WHHL rabbits and are therefore interchangeable....

  17. Comparison of the effects of fish oil and olive oil on blood lipids and aortic atherosclerosis in Watanabe heritable hyperlipidaemic rabbits

    DEFF Research Database (Denmark)

    Mortensen, Alicja; Hansen, Birgit Fischer; Hansen, Jørgen Fischer

    1998-01-01

    To compare the effects of fish oil and olive oil on the development of atherosclerosis in Watanabe heritable hyperlipidaemic (WHHL) rabbits, 6-week-old animals were given a daily dose (1.5 ml/kg body weight) of fish oil (n 10) or olive oil (n 10) by oral administration for 16 weeks. Plasma...... of treatment, and throughout the study thereafter, blood lipids were significantly (P oil group than in the olive-oil group (cholesterol: 17.0 v. 30.3 mmol/l, triacylglycerols 2.97 v. 6.25 mmol/l, at termination). In the fish-oil group cholesterol was significantly lower...... in intermediate-density lipoproteins (2.69 v. 6.76 mmol/l) and VLDL (3.36 v. 11.51 mmol/l). Triacylglycerol levels of intermediate-density lipoproteins and VLDL in the fish-oil group were also significantly lower when compared with the olive-oil group (0.54 v 1.36 mmol/l and 0.92 v. 2.87 mmol/l respectively...

  18. Growth indices and economic implications of weaned rabbits fed ...

    African Journals Online (AJOL)

    Growth indices and economic implications of weaned rabbits fed Leucaena ... the 13.47g weight gain on rabbits fed the control diet but statistically (P<0.05) lower ... economic efficiency of 0.35 and 194.44%, respectively indicated the optimal ...

  19. Testicular Morphometry and Sperm Quality of Rabbit Bucks Fed ...

    African Journals Online (AJOL)

    Testicular Morphometry and Sperm Quality of Rabbit Bucks Fed Graded Levels ... for their nutritional and medicinal values and are rich in minerals and vitamins. ... used to evaluate the effect of Moringa oleifera leaf meal (MOLM) on testicular ...

  20. Performance and Carcass Characteristics of Rabbits Fed Oil Supplemented Diets

    Directory of Open Access Journals (Sweden)

    Jamal Abo OMAR

    2016-02-01

    Full Text Available The objective of this study is to investigate the effects of feeding different dietary fat supplements in the finisher rations of Baladi rabbits, including sesame oil (SO, olive oil sediments (OOS, and poultry grease (PG, in comparison to the traditional oil supplement, the soybean soap stock oil (SS, on growth performance, blood lipid profile, dressing percentage and carcass cut, and meat quality: water holding capacity (WHC and cell forming unit (CFU. A total of 48 Baladi rabbits were used, with individual body weights (BW of 519 ± 22 g at the beginning of the experiment. Rabbits were randomly divided into 4 groups of 12, and those in each group were individually fed cereal grain-soybean meal (SBM with a fixed amount (i.e. 30 g/kg dry matter (DM of oil, being either soybean oil (SOY, olive oil sediments (OOS, recycled restaurant oil (RRO, or poultry grease (PG. All rations were isonitrogenous and contained iso-metabolizable energy (ME. At the end of the 44 day feeding trial, all animals were slaughtered. Rabbits fed a SOY supplemented diet consumed more (P < 0.05 feed than those fed the OOS, RRO, or PG supplemented diets. However, rabbits fed the SOY had a better (P < 0.05 feed conversion ratio than rabbits fed the OOS, PG, or RRO diets. Oil source had no effect on carcass components weights. Liver was heavier (P < 0.05 in rabbits fed the SOY supplemented diet. However, the RRO fed rabbits had heavier (P < 0.05 small intestine, large intestine and cecum. In conclusion, the positive effects of the tested oil supplements (i.e. RRO, OOS, PG on the studied performance and carcass traits is encouraging, but more investigation is needed to identify the optimal levels for these supplements in various diets of local rabbits.

  1. Urotensin II promotes atherosclerosis in cholesterol-fed rabbits.

    Directory of Open Access Journals (Sweden)

    Yafeng Li

    Full Text Available Urotensin II (UII is a vasoactive peptide composed of 11 amino acids that has been implicated to contribute to the development of cardiovascular disease. The purpose of this study was to investigate whether UII affects the development of atherosclerosis in cholesterol-fed rabbits. UII was infused for 16 weeks through an osmotic mini-pump into male Japanese White rabbits fed on a high-cholesterol diet. Plasma lipids and body weight were measured every 4 weeks. Aortic atherosclerotic lesions along with cellular components, collagen fibers, matrix metalloproteinase-1 and -9 were examined. Moreover, vulnerability index of atherosclerotic plaques was evaluated. UII infusion significantly increased atherosclerotic lesions within the entire aorta by 21% over the control (P = 0.013. Atherosclerotic lesions were increased by 24% in the aortic arch (P = 0.005, 11% in the thoracic aorta (P = 0.054 and 18% in the abdominal aorta (P = 0.035. These increases occurred without changes in plasma levels of total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, triglycerides or body weight. Immunohistochemical staining revealed that macrophages and matrix metalloproteinase-9 were significantly enhanced by 2.2-fold and 1.6-fold in UII group. In vitro studies demonstrated that UII up-regulated the expression of vascular cell adhesion protein-1 and intercellular adhesion molecule-1 in human umbilical vein endothelial cells, which was inhibited by the UII receptor antagonist urantide. In conclusion, our results showed that UII promotes the development of atherosclerotic lesions and destabilizes atherosclerotic plaques in cholesterol-fed rabbits.

  2. Immune Response and Pasteurella Resistance in Rabbits Fed Diets Containing Various Amounts of Black Cumin Seeds

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    Nabiela M. El Bagir

    2010-01-01

    Full Text Available Problem statement: The consumption of black cumin (Nigella sativa seed has immunomodulatory and anti-bacterial activity, but in rabbits this had not yet been tested. Approach: In the present studies, rabbits were fed diets without or with black cumin seed and antibody production, phagocytotic activity, hypersensitivity and resistance against Pasteurellosis were assessed. Results: Feeding black cumin seed significantly increased serum concentrations of antibodies in response to intramusculary injected serum bovine albumin. Blood derived from rabbits fed the diets containing either 15 or 20% black cumin seed significantly reduced the growth of Staphylococcus aureus on sheep-blood agar plates. Skin thickness as index of hypersensitivity towards tuberculin was significantly reduced at 48 h after intradermal injection of the agent. Ingestion of black cumin seed significantly extended survival time after intraperitoneal administration of Pasteurella multocida. Conclusion: The feeding of black cumin seed to rabbits stimulated their immune system, but did not enhance inflammation.

  3. Cilostazol inhibits accumulation of triglyceride in aorta and platelet aggregation in cholesterol-fed rabbits.

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    Hideki Ito

    Full Text Available Cilostazol is clinically used for the treatment of ischemic symptoms in patients with chronic peripheral arterial obstruction and for the secondary prevention of brain infarction. Recently, it has been reported that cilostazol has preventive effects on atherogenesis and decreased serum triglyceride in rodent models. There are, however, few reports on the evaluation of cilostazol using atherosclerotic rabbits, which have similar lipid metabolism to humans, and are used for investigating the lipid content in aorta and platelet aggregation under conditions of hyperlipidemia. Therefore, we evaluated the effect of cilostazol on the atherosclerosis and platelet aggregation in rabbits fed a normal diet or a cholesterol-containing diet supplemented with or without cilostazol. We evaluated the effects of cilostazol on the atherogenesis by measuring serum and aortic lipid content, and the lesion area after a 10-week treatment and the effect on platelet aggregation after 1- and 10-week treatment. From the lipid analyses, cilostazol significantly reduced the total cholesterol, triglyceride and phospholipids in serum, and moreover, the triglyceride content in the atherosclerotic aorta. Cilostazol significantly reduced the intimal atherosclerotic area. Platelet aggregation was enhanced in cholesterol-fed rabbits. Cilostazol significantly inhibited the platelet aggregation in rabbits fed both a normal diet and a high cholesterol diet. Cilostazol showed anti-atherosclerotic and anti-platelet effects in cholesterol-fed rabbits possibly due to the improvement of lipid metabolism and the attenuation of platelet activation. The results suggest that cilostazol is useful for prevention and treatment of atherothrombotic diseases with the lipid abnormalities.

  4. Anaerobic facultative bacteria isolated from the gut of rabbits fed different diets.

    Science.gov (United States)

    Canganella, F; Zirletta, G; Gualterio, L; Massa, S; Trovatelli, L D

    1992-11-01

    Anaerobic facultative bacteria colonizing the intestinal tract of conventional rabbits fed three different diets (standard pellet, hay and pellet/hay mixture) were enumerated in brain heart infusion agar. Colony counts recovered from homogenized samples of small intestine, caecum and rectum differed with reference to the diet given. Among anaerobic groups, identified from rabbit fed pellet/hay mixture, Enterococci (E. faecalis, E. avium, E. faecium and E. durans) represented the predominant flora. Enterobacters (E. cloacae and E. aerogenes) accounted for about 10 to 25% of the bacteria in the rectum and colon respectively, whereas Staphylococci (S. intermedius, S. epidermidis and S. lentus) represented 11% of the bacteria isolated from colon.

  5. Erythrocyte Osmotic Fragility and Excitability Score in Rabbit fed ...

    African Journals Online (AJOL)

    olayemitoyin

    protect cells against oxidative stress in rats (Wang et al., 2000) and ... building for easy and effective cross-ventilation, and the cages were ... Graded level of Hibiscus sabdariffa alters erythrocyte membrane stability in rabbit. 114 ..... Haematological and serum biochemical response ... Vitamin C in human health and disease ...

  6. Digestion, growth performance and caecal fermentation in growing rabbits fed diets containing foliage of browse trees

    Directory of Open Access Journals (Sweden)

    S.H. Abu Hafsa

    2016-12-01

    Full Text Available This study aimed to evaluate the effect of feeding dried foliage (leaves and petioles of Acacia saligna, Leucaena leucocephala or Moringa oleifera on the performance, digestibility, N utilisation, caecal fermentation and microbial profiles in New Zealand White (NZW rabbits. One hundred weaned male NZW rabbits weighing 819.2±16.6 g and aged 35±1 d were randomly allocated into 4 groups of 25 rabbits each. Rabbits were fed on pelleted diets containing 70% concentrate mixture and 30% Egyptian berseem (Trifolium alexandrinum hay (Control diet or one of the other 3 experimental diets, where 50% of berseem hay was replaced with A. saligna (AS, L. leucocephala (LL or M. oleifera (MO. Compared to Control diet, decreases in dry matter (DM; P=0.004, organic matter (P=0.028, crude protein (CP; P=0.001, neutral detergent fibre (P=0.033 and acid detergent fibre (P=0.011 digestibility were observed with the AS diet. However, DM and CP digestibility were increased by 3% with the MO diet, and N utilisation was decreased (P<0.05 with AS. Rabbits fed AS and LL diets showed decreased (P=0.001 average daily gain by 39 and 7%, respectively vs. Control. Feed conversion was similar in Control and MO rabbits, whereas rabbits fed AS diet ate up to 45% more feed (P=0.002 than Control rabbits to gain one kg of body weight. Caecal ammonia-N was increased (P=0.002 with LL, while acetic acid was decreased (P=0.001 with AS diet vs. other treatments. Caecal E. coli and Lactobacillus spp. bacteria counts were decreased with MO by about 44 and 51%, respectively, vs. Control. In conclusion, under the study conditions, tree foliage from M. oleifera and L. leucocephala are suitable fibrous ingredients to be included up to 150 g/kg in the diets of growing rabbits, and can safely replace 50% of berseem hay in diets of NZW rabbits without any adverse effect on their growth performance. Foliage from M. oleifera had a better potential as a feed for rabbits than that from L

  7. Comparison of behaviour, performance and mortality in restricted and ad libitum-fed growing rabbits.

    Science.gov (United States)

    Dalmau, A; Abdel-Khalek, A M; Ramon, J; Piles, M; Sanchez, J P; Velarde, A; Rafel, O

    2015-07-01

    The objective of this study was to determine whether rabbits fed in a restricted regimen (75%) showed increased competition for feeding, drinking and use of specific areas of the cages as compared with those provided feed ad libitum. This evaluation was carried out by measuring their space utilisation in the cage, the incidence of agonistic behaviour and rates of mortality. In total, 504 rabbits between 31 and 66 days of age were used in this study. A total of 200 heavy-weight rabbits and 56 light-weight rabbits were randomly housed in 32 cages, each cage containing eight rabbits: 25 cages housing heavy rabbits and seven cages housing the light-weight ones. They were all fed ad libitum (AD). In addition, a total of 208 heavy-weight rabbits and 40 light-weight rabbits were randomly housed in 31 cages, each of them containing eight rabbits: 26 cages housing heavy weight rabbits and five cages housing light-weight ones. They were all fed a restricted diet (R) regimen. The restriction was calculated to be 75% of the feed consumed by the AD group. The total space available in the cage was 3252 cm(2), with a stocking density of 24.6 animals/m(2). Animals between 32 and 60 days of age from 20 different cages were observed nine times per week (morning or afternoon) by means of scan and focal sampling by one observer. During each period, cages were assessed for 5 min, registering every minute the position of all the animals in relation to Area A (feeder), Area B (central part) or Area C (back and drinker area). The incidence of agonistic behaviour such as displacement, biting and jumping on each other was also assessed. Performance variables such as daily gain and feed conversion ratio, in addition to general health status and mortality rates, were recorded for all rabbits. When the rabbits were under restricted feeding, the competition for feed and drink increased with clear signs of agonistic behaviour such as biting, displacement and animals jumping on top of each other

  8. Modelling atherosclerosis by proteomics: Molecular changes in the ascending aortas of cholesterol-fed rabbits.

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    Xu, Jingshu; Jüllig, Mia; Middleditch, Martin J; Cooper, Garth J S

    2015-09-01

    The cholesterol-fed rabbit is commonly used as a model to study the vascular effects of hypercholesterolemia and resulting atherosclerotic lesions. Here we undertook a proteomic case-control investigation of ascending aortas from male New Zealand White rabbits after 10 weeks on a high-cholesterol (2% w/w) diet (HCD, n = 5) or control diet (n = 5), in order to determine the changes in response to the HCD. Histology confirmed intimal thickening in the HCD group consistent with atherosclerosis, and LC-MS/MS analysis of individually-obtained ascending aortic extracts labelled with isobaric (iTRAQ) tags enabled the identification and quantitation of 453 unique proteins above the 1% false discovery rate threshold. Of 67 proteins showing significant differences in relative abundance (p atherosclerosis. This and additional novel observations merit further investigation as these perturbations may play important and as yet undiscovered roles in the pathogenesis of atherosclerosis in rabbits as well as humans.

  9. Hypolipidemic and Antioxidant Effects of Dandelion (Taraxacum officinale) Root and Leaf on Cholesterol-Fed Rabbits

    OpenAIRE

    Seong-Il Lim; Young Kyung Rhee; Chang-Won Cho; Joo Hyuk Yim; Ok-Hwan Lee; Ung-Kyu Choi; Young-Chan Kim

    2010-01-01

    Dandelion (Taraxacum officinale), an oriental herbal medicine, has been shown to favorably affect choleretic, antirheumatic and diuretin properties. Recent reports have indicated that excessive oxidative stress contributes to the development of atherosclerosis-linked metabolic syndrome. The objective of this current study was to investigate the possible hypolipidemic and antioxidative effects of dandelion root and leaf in rabbits fed with a high-cholesterol diet. A group of twenty eight male ...

  10. Illumina Miseq platform analysis caecum bacterial communities of rex rabbits fed with different antibiotics.

    Science.gov (United States)

    Zou, Fuqin; Zeng, Dong; Wen, Bin; Sun, Hao; Zhou, Yi; Yang, Mingyue; Peng, Zhirong; Xu, Shuai; Wang, Hesong; Fu, Xiangchao; Du, Dan; Zeng, Yan; Zhu, Hui; Pan, Kangcheng; Jing, Bo; Wang, Ping; Ni, Xueqin

    2016-12-01

    Antibiotics have been widely used for the prevention and the treatment of diseases to humans and animals, and they have fed additives for agricultural animals to promote growth. However, there is a growing concern over the practice due to its side effects on intestinal microbial communities which plays a vital role in animals' health. To investigate the effect of antibiotics on the bacterial population of the caecum in rex rabbits, 80 rex rabbits were randomly divided into four groups: control group (B, basal diet), chlortetracycline group (C, 50 mg/kg), colistin sulfate group (S, 20 mg/kg) and zinc bacitracin group (Z, 40 mg/kg). Caecum microbial communities of rex rabbits from the four groups were analyzed through Illumina Miseq platform after being fed 28 days. The results showed that most obtained sequences belongs to Firmicutes followed by Bacteroidetes, and the ratio of Bacteroidetes/Firmicutes in C group (42.31 %) was higher than that in Z group (21.84 %). Zinc bacitracin supplementation caused a significant decreased of the Proteobacteria phylum and Lactobacillus spp. (P rex rabbits, and we also found C group had a similarity caecum bacteria structure with B group which was probably due to the high levels of chlortetracycline resistance.

  11. Early extracellular and cellular lipid deposits in aorta of cholesterol-fed rabbits.

    Science.gov (United States)

    Guyton, J. R.; Klemp, K. F.

    1992-01-01

    Subendothelial accumulation of extracellular liposomes rich in unesterified cholesterol has been described as an early feature of atherosclerosis induced by cholesterol feeding in rabbits. Beta-very-low-density lipoproteins, however, the presumed source of atherogenic lipid in this animal model, contain mostly esterified cholesterol. The purpose of this study was to test for the presence of extracellular neutral lipid deposits consistent with esterified cholesterol, by employing new electron microscopic techniques. Rabbits were fed 0.5% cholesterol, 5% butter for 0, 1, 2, and 4 weeks. The lipid-preserving ultrastructural techniques showed, in control and atherosclerotic rabbit arteries, neutral lipid droplets adherent to the endothelial luminal surface. After 1 to 2 weeks, subendothelial extracellular deposits of mostly membranous lipid appeared; these deposits contained variable amounts of neutral lipid. At the same time, cytoplasmic neutral lipid droplets appeared in smooth muscle cells and in a small number of subendothelial macrophagelike cells. After 4 weeks, monocytic infiltration and macrophage foam cell development were prominent, but abundant extracellular lipid deposits also were found. Therefore, in arteries of cholesterol-fed rabbits, deposition of membranous and neutral lipid in the extracellular space and neutral lipid accumulation in resident arterial cells are early and probably independent events, both occurring before monocytic infiltration of the arterial intima. Images Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Figure 9 Figure 10 Figure 11 PMID:1415485

  12. Hypolipidemic and Antioxidant Effects of Dandelion (Taraxacum officinale Root and Leaf on Cholesterol-Fed Rabbits

    Directory of Open Access Journals (Sweden)

    Seong-Il Lim

    2010-01-01

    Full Text Available Dandelion (Taraxacum officinale, an oriental herbal medicine, has been shown to favorably affect choleretic, antirheumatic and diuretin properties. Recent reports have indicated that excessive oxidative stress contributes to the development of atherosclerosislinked metabolic syndrome. The objective of this current study was to investigate the possible hypolipidemic and antioxidative effects of dandelion root and leaf in rabbits fed with a high-cholesterol diet. A group of twenty eight male rabbits was divided into four subgroups; a normal diet group, a high-cholesterol diet group, a high-cholesterol diet with 1% (w/w dandelion leaf group, and a high-cholesterol diet with 1% (w/w dandelion root group. After the treatment period, the plasma antioxidant enzymes and lipid profiles were determined. Our results show that treatment with dandelion root and leaf positively changed plasma antioxidant enzyme activities and lipid profiles in cholesterol-fed rabbits, and thus may have potential hypolipidemic and antioxidant effects. Dandelion root and leaf could protect against oxidative stress linked atherosclerosis and decrease the atherogenic index.

  13. Hypolipidemic and antioxidant effects of dandelion (Taraxacum officinale) root and leaf on cholesterol-fed rabbits.

    Science.gov (United States)

    Choi, Ung-Kyu; Lee, Ok-Hwan; Yim, Joo Hyuk; Cho, Chang-Won; Rhee, Young Kyung; Lim, Seong-Il; Kim, Young-Chan

    2010-01-06

    Dandelion (Taraxacum officinale), an oriental herbal medicine, has been shown to favorably affect choleretic, antirheumatic and diuretin properties. Recent reports have indicated that excessive oxidative stress contributes to the development of atherosclerosis-linked metabolic syndrome. The objective of this current study was to investigate the possible hypolipidemic and antioxidative effects of dandelion root and leaf in rabbits fed with a high-cholesterol diet. A group of twenty eight male rabbits was divided into four subgroups; a normal diet group, a high-cholesterol diet group, a high-cholesterol diet with 1% (w/w) dandelion leaf group, and a high-cholesterol diet with 1% (w/w) dandelion root group. After the treatment period, the plasma antioxidant enzymes and lipid profiles were determined. Our results show that treatment with dandelion root and leaf positively changed plasma antioxidant enzyme activities and lipid profiles in cholesterol-fed rabbits, and thus may have potential hypolipidemic and antioxidant effects. Dandelion root and leaf could protect against oxidative stress linked atherosclerosis and decrease the atherogenic index.

  14. Antihyperlipidemic activity of adenosine triphosphate in rabbits fed a high-fat diet and hyperlipidemic patients.

    Science.gov (United States)

    Zhang, Lianshan; Liang, Libin; Tong, Tong; Qin, Yuguo; Xu, Yanping; Tong, Xinglong

    2016-10-01

    Context Recently, adenosine triphosphate (ATP) was occasionally found to decrease the triglyceride (TG) levels in several hyperlipidemic patients in our clinical practice. Objective The study investigates the anti-hyperlipidemic effects of ATP in a high-fat fed rabbit model and hyperlipidemic patients. Materials and methods Twenty-four rabbits were randomly divided into three groups of eight animals each as follows: normal diet, high-fat diet and high-fat diet + ATP group. ATP supplementation (40 mg/day) was started at the 20th day and lasted for 10 days. Serum concentrations of total cholesterol (TC), TG, LDL-C, HDL-C were measured on the 20th day and 30th day. Heart, liver and aorta were subjected histopathological examination. Twenty outpatients diagnosed primary hyperlipidemia took ATP at a dose of 60 mg twice a day for 1 week. Results Feeding rabbits with a high-fat diet resulted in a significant elevation of lipid parameters including TC, TG, LDL-C, VLDL-C compared to the normal diet group (p ATP treatment significantly decreased serum TG level (p ATP significantly reduced the thickness of fat layer in cardiac epicardium (p ATP for 1 week, hyperlipidemia patients exhibited a significant decrease of TG (p ATP selectively decreases serum TG levels in high-fat diet rabbits and hyperlipidemic patients. Therefore, ATP supplementation may provide an effective approach to control TG level.

  15. PATULIN - INDUCED CHANGES IN HAEMATOLOGICAL PARAMETERS OF RABBITS FED BY STRAWBERRY LEAVES AFTER 3 WEEKS EXPOSURE

    Directory of Open Access Journals (Sweden)

    Jana Emrichova

    2014-02-01

    Full Text Available The subject of the present study was to determinate the effect of strawberry leaves inclusion into the feed mixture and single dose of patulin on haematological parameters of rabbits: white blood cell count (WBC, lymphocytes count (LYM, medium size cell count (MID, granulocytes count (GRA, lymphocyte percentage (LYM%, medium size cell percentage (MI%, granulocytes percentage (GRA%, red blood cell count (RBC, haemoglobin (HGB, haematocrit (HCT, mean corpuscular volume (MCV, mean corpuscular haemoglobin (MCH, mean corpuscular haemoglobin concentration (MCHC, red cell distribution width (RDWc, platelet count (PLT, platelet percentage (PCT, mean platelet volume (MPV and platelet distribution width (PDWc. Fifteen rabbits of broiler line Californian were used in this experiment. Animals were divided into four groups, one control group C (n =3 and three experimental groups E1, E2 and E3 (n = 4 in each group. Rabbits were fed with a granular feed mixture (FM with strawberry leaves in various doses and all groups received patulin in injectable form at 10 µg.kg-1 for 21 days 2 times a week. The investigation was performed with haematology analyzer Abacus junior VET (Diatron®, Vienna, Austria. Significant decrease in MI% and MID in experimental group E3 in comparison with the control group was found. The values of other parameters (WBC, LYM, LY%, GRA, GR%, RBC, HGB, HCT, MCV, MCH, MCHC, RDWc, PLT, MPV and PDWc in all groups corresponded with normal haematological values in rabbit´s blood.

  16. Blood values of adult captive cheetahs (Acinonyx jubatus) fed either supplemented beef or whole rabbit carcasses.

    Science.gov (United States)

    Depauw, Sarah; Hesta, M; Whitehouse-Tedd, K; Stagegaard, J; Buyse, J; Janssens, G P J

    2012-01-01

    This study evaluated nutrient intake and relevant blood parameters of 14 captive cheetahs, randomly assigned to a meat-only diet (supplemented beef, SB) or a whole prey diet (whole rabbit, WR) for 4 weeks each. Despite a higher food intake, daily metabolizable energy intake was lower when fed WR (308 kJ BW(-1) ) compared with SB (347 kJ BW(-1) ) (P = 0.002). The ratio of protein to fat was markedly lower for WR (2.3:1) compared with SB (8.8:1), which was reflected in higher serum urea levels when fed SB (P = 0.033), and a tendency for elevated cholesterol levels when fed WR (P = 0.055). Taurine intake of cheetahs fed WR was low (0.06% on DM basis); however, analytical error during taurine analysis cannot be ruled out. Feeding WR resulted in a well-balanced mineral intake, in contrast to SB. The latter provided a low calcium:phosphorus ratio (1:2.3), thereby increasing the risk of metabolic bone disease. The high zinc content of SB (200 mg/kg DM), compared with WR (94 mg/kg DM), was reflected in higher serum zinc concentrations (P = 0.011). Feeding WR resulted in an increase in serum vitamin A (P = 0.011). Therefore, the risk of hypervitaminosis A in captive cheetahs when fed WR exclusively on a long-term basis should be evaluated. Our findings suggest that neither diet is likely to provide appropriate nutrition to captive cheetahs when fed exclusively.

  17. Antihyperlipidemic Effects of Sesamum indicum L. in Rabbits Fed a High-Fat Diet

    Directory of Open Access Journals (Sweden)

    Sedigheh Asgary

    2013-01-01

    Full Text Available The present study aimed to investigate the anti-hyperlipidemic effects of sesame in a high-fat fed rabbit model. Animals were randomly divided into four groups of eight animals each for 60 days as follows: normal diet, hypercholesterolemic diet (1% cholesterol, hypercholesterolemic diet (1% cholesterol + sesame seed (10%, and hypercholesterolemic diet (1% cholesterol + sesame oil (5%. Serum concentrations of total cholesterol, LDL-C, HDL-C, triglycerides, apoA and apoB, SGOT, SGPT, glucose and insulin were measured at the end of supplementation period in all studied groups. Hypercholesterolemic feeding resulted in a significant elevation of TC, TG, LDL-C, HDL-C, SGOT and SGPT as compared to the normocholesterolemic diet group (P0.05. In contrast, rabbits supplemented with sesame oil were found to have lower circulating concentrations of TC, LDL-C, HDL-C, SGOT and SGPT (P0.05. Supplementation with sesame oil, but not sesame seed, can ameliorate serum levels of lipids and hepatic enzymes in rabbits under a high-fat diet.

  18. Antihyperlipidemic effects of Sesamum indicum L. in rabbits fed a high-fat diet.

    Science.gov (United States)

    Asgary, Sedigheh; Rafieian-Kopaei, Mahmoud; Najafi, Somayeh; Heidarian, Esfandiar; Sahebkar, Amirhossein

    2013-01-01

    The present study aimed to investigate the anti-hyperlipidemic effects of sesame in a high-fat fed rabbit model. Animals were randomly divided into four groups of eight animals each for 60 days as follows: normal diet, hypercholesterolemic diet (1% cholesterol), hypercholesterolemic diet (1% cholesterol) + sesame seed (10%), and hypercholesterolemic diet (1% cholesterol) + sesame oil (5%). Serum concentrations of total cholesterol, LDL-C, HDL-C, triglycerides, apoA and apoB, SGOT, SGPT, glucose and insulin were measured at the end of supplementation period in all studied groups. Hypercholesterolemic feeding resulted in a significant elevation of TC, TG, LDL-C, HDL-C, SGOT and SGPT as compared to the normocholesterolemic diet group (P sesame seed did not cause any significant alteration in lipid profile parameters, apolipoproteins, hepatic transaminases, glucose and insulin as compared to the hypercholesterolemic diet group (P > 0.05). In contrast, rabbits supplemented with sesame oil were found to have lower circulating concentrations of TC, LDL-C, HDL-C, SGOT and SGPT (P 0.05). Supplementation with sesame oil, but not sesame seed, can ameliorate serum levels of lipids and hepatic enzymes in rabbits under a high-fat diet.

  19. Effects of antioxidant vitamins C and E on atherosclerosis in lipid-fed rabbits.

    Science.gov (United States)

    Sun, Y P; Zhu, B Q; Sievers, R E; Norkus, E P; Parmley, W W; Deedwania, P C

    1998-03-01

    Antioxidant vitamins are hypothesized to help prevent atherosclerosis by blocking lipoprotein oxidation. We investigated the effects of dietary vitamins C and E on atherosclerosis in rabbits. Forty New Zealand male rabbits were divided into 4 groups: 0.3% cholesterol diet with (LV) and without vitamin (LC), and 0.5% cholesterol diet with (HV) and without vitamins (HC). The treated groups consumed 137 +/- 8 mg/day vitamin C and 80 +/- 4 mg/day vitamin E for 10 weeks. Vitamin treatment did not significantly affect serum lipids. Alpha-tocopherol values were significantly higher in both serum (mg/dl) and omental fat (microg/g) among the treated rabbits (3.9 +/- 0.5 and 31.6 +/- 2.1 for LV, 1.7 +/- 0.2 and 12.1 +/- 1.9 for LC, 5.6 +/- 0.8 and 51.3 +/- 9.3 for HV and 1.9 +/- 0.3 and 8.2 +/- 0.4 for HC; p Vitamin treatment did not affect the percent of surface lesions in the aorta and pulmonary artery (23.8 +/- 5.2 and 20.1 +/- 3.3% for LV, 19.8 +/- 5.6 and 23.2 +/- 3.5% for LC, 28.1 +/- 6.5 and 51.1 +/- 4.2% for HV and 32.4 +/- 5.5 and 43.7 +/- 3.9% for HC, respectively; p = 0.981 and p = 0.562. Although significantly higher values of alpha-tocopherol were found in both serum and omental fat, antioxidant vitamins C and E did not demonstrate a significant protective effect on atherosclerosis in lipid-fed rabbits during the 10-week study period.

  20. Gonadal and extra-gonadal sperm reserves and sperm production of pubertal rabbits fed dietary fumonisin B1.

    Science.gov (United States)

    Ewuola, E O; Egbunike, G N

    2010-06-01

    The influence of fumonisin B(1) (a mycotoxin produced by Fusarium verticillioides) on sperm reserves and production of crossbred pubertal rabbits was studied using an experimental model that lasted 28 weeks. Forty-eight male rabbits, 7 weeks old and with average weight of 757.50+/-0.50 g, were allotted to four dietary fumonisin B(1) concentrations of 0.13, 5.0, 7.5 and 10.0 mg kg(-1) constituting diets 1 (control), 2, 3 and 4, respectively. The paired testes weight of rabbits fed diet 3 was significantly (Panimals were significantly (Pproduction of the animals fed diets 2, 3 and 4 declined significantly to 67, 59 and 36% relative to those animals fed the control diet. This study suggests that exposure of breeding male rabbits to diets contaminated with fumonisin B(1) up to 7.5 mg fumonisin B(1)/kg will depress testicular and epididymal sperm reserves and sperm production and potentially impair reproduction in the animals. Copyright (c) 2010 Elsevier B.V. All rights reserved.

  1. Effect of curcumin on LDL oxidation in vitro, and lipid peroxidation and antioxidant enzymes in cholesterol fed rabbits.

    Science.gov (United States)

    Mahfouz, Mohamedain M; Zhou, Qi; Kummerow, Fred A

    2011-11-01

    In this study we examined the antioxidant effect of curcumin on lipid oxidation in vitro and in vivo. In vitro, curcumin at 5 microgM concentration completely prevented low-density lipoprotein (LDL) oxidation by CuS0(4), indicating that curcumin is an effective antioxidant in vitro. In vivo, feeding a pure cholesterol (PC)-rich diet to rabbits significantly increased the plasma and liver lipids as well as thiobarbituric acid reactive substances (TBARS) levels. Addition of curcumin to the PC diet did not show any effect on either plasma lipid and TBARS or liver lipids. Liver TBARS tended to decrease but that decrease was not significant. Erythrocyte glutathione peroxidase (GSH-Px) activity was significantly decreased while catalase activity was significantly increased in rabbits fed a PC diet. The addition of curcumin to a PC diet did not show any significant effect on erythrocyte enzyme activities compared to the rabbits fed a PC diet. The liver GSH-Px and catalase activities were significantly decreased in rabbits fed a PC diet, but the addition of curcumin to the PC diet enhanced the liver GSH-Px activity, which became nonsignificantly different from the control group. These results were discussed considering that curcumin may not be well absorbed and it did not reach a level high enough in vivo to overcome the severe hypercholesterolemia and oxidative stress produced by the PC-rich diet.

  2. Immunotherapy for Alzheimer's disease: harnessing our knowledge of T cell biology using a cholesterol-fed rabbit model.

    Science.gov (United States)

    Coico, Richard; Woodruff-Pak, Diana S

    2008-12-01

    This timely special issue of the Journal of Alzheimer's Disease provides the opportunity to examine interfaces between basic science and clinical medicine using animal models to develop more effective therapies for the treatment and, ideally, prevention of Alzheimer's disease (AD). That some patients with AD enrolled in a clinical trial to inoculate against amyloid-beta (Abeta) experienced a misdirected polarization of Th cells reminds us that our knowledge of T cell biology, immune regulation, and the precise functional properties of adjuvants is incomplete. We review this knowledge and consider the advantages of the rabbit for immunological studies. The langomorph species is proximate to primates on the phylogenetic scale, its amino acid sequence of Abeta is 97% identical to the human Abeta sequence, and the rabbit model system is extensively characterized on a form of associative learning with parallels in normal aging in rabbits and humans that is severely impaired in human AD. Cholesterol-fed rabbits treated with Abeta immunotherapy generate high titer anti-Abeta responses. The cholesterol-fed rabbit model of AD with its close parallels to human genetics and physiology, along with its validity from molecular to cognitive levels as a model of human AD, provides a promising vehicle for development of immunotherapies.

  3. Hyperlipidemia-associated gene variations and expression patterns revealed by whole-genome and transcriptome sequencing of rabbit models

    Science.gov (United States)

    Wang, Zhen; Zhang, Jifeng; Li, Hong; Li, Junyi; Niimi, Manabu; Ding, Guohui; Chen, Haifeng; Xu, Jie; Zhang, Hongjiu; Xu, Ze; Dai, Yulin; Gui, Tuantuan; Li, Shengdi; Liu, Zhi; Wu, Sujuan; Cao, Mushui; Zhou, Lu; Lu, Xingyu; Wang, Junxia; Yang, Jing; Fu, Yunhe; Yang, Dongshan; Song, Jun; Zhu, Tianqing; Li, Shen; Ning, Bo; Wang, Ziyun; Koike, Tomonari; Shiomi, Masashi; Liu, Enqi; Chen, Luonan; Fan, Jianglin; Chen, Y. Eugene; Li, Yixue

    2016-01-01

    The rabbit (Oryctolagus cuniculus) is an important experimental animal for studying human diseases, such as hypercholesterolemia and atherosclerosis. Despite this, genetic information and RNA expression profiling of laboratory rabbits are lacking. Here, we characterized the whole-genome variants of three breeds of the most popular experimental rabbits, New Zealand White (NZW), Japanese White (JW) and Watanabe heritable hyperlipidemic (WHHL) rabbits. Although the genetic diversity of WHHL rabbits was relatively low, they accumulated a large proportion of high-frequency deleterious mutations due to the small population size. Some of the deleterious mutations were associated with the pathophysiology of WHHL rabbits in addition to the LDLR deficiency. Furthermore, we conducted transcriptome sequencing of different organs of both WHHL and cholesterol-rich diet (Chol)-fed NZW rabbits. We found that gene expression profiles of the two rabbit models were essentially similar in the aorta, even though they exhibited different types of hypercholesterolemia. In contrast, Chol-fed rabbits, but not WHHL rabbits, exhibited pronounced inflammatory responses and abnormal lipid metabolism in the liver. These results provide valuable insights into identifying therapeutic targets of hypercholesterolemia and atherosclerosis with rabbit models. PMID:27245873

  4. Rabbit models for the study of human atherosclerosis: from pathophysiological mechanisms to translational medicine.

    Science.gov (United States)

    Fan, Jianglin; Kitajima, Shuji; Watanabe, Teruo; Xu, Jie; Zhang, Jifeng; Liu, Enqi; Chen, Y Eugene

    2015-02-01

    Laboratory animal models play an important role in the study of human diseases. Using appropriate animals is critical not only for basic research but also for the development of therapeutics and diagnostic tools. Rabbits are widely used for the study of human atherosclerosis. Because rabbits have a unique feature of lipoprotein metabolism (like humans but unlike rodents) and are sensitive to a cholesterol diet, rabbit models have not only provided many insights into the pathogenesis and development of human atherosclerosis but also made a great contribution to translational research. In fact, rabbit was the first animal model used for studying human atherosclerosis, more than a century ago. Currently, three types of rabbit model are commonly used for the study of human atherosclerosis and lipid metabolism: (1) cholesterol-fed rabbits, (2) Watanabe heritable hyperlipidemic rabbits, analogous to human familial hypercholesterolemia due to genetic deficiency of LDL receptors, and (3) genetically modified (transgenic and knock-out) rabbits. Despite their importance, compared with the mouse, the most widely used laboratory animal model nowadays, the use of rabbit models is still limited. In this review, we focus on the features of rabbit lipoprotein metabolism and pathology of atherosclerotic lesions that make it the optimal model for human atherosclerotic disease, especially for the translational medicine. For the sake of clarity, the review is not an attempt to be completely inclusive, but instead attempts to summarize substantial information concisely and provide a guideline for experiments using rabbits.

  5. Hazelnut Oil Administration Reduces Aortic Cholesterol Accumulation and Lipid Peroxides in the Plasma, Liver, and Aorta of Rabbits Fed a High-cholesterol Diet

    National Research Council Canada - National Science Library

    HATIPOGLU, Aydan; KANBAGLI, Öznur; BALKAN, Jale; KÜÇÜK, Mutlu; ÇEVIKBAS, Ugur; AYKAÇ-TOKER, Gülçin; BERKKAN, Hakan; UYSAL, Müjdat

    2004-01-01

    Hazelnut oil (HO) is rich in monounsaturated fatty acids and antioxidants. We wanted to investigate the effect of HO on lipid levels and prooxidant-antioxidant status in rabbits fed a high-cholesterol (HC) diet...

  6. A Decreased Responsiveness of Platelet to Nitric Oxide in Cholesterol-Fed Rabbits

    Institute of Scientific and Technical Information of China (English)

    SUNJing; ZHANGAi-xia; LUOChun-xia; WANGWei; SUNYong-jun; ZHUDong-ya

    2004-01-01

    To determine whether endothelial dysfunction leads to an abnormal responsiveness of platelet to nitric oxide(NO)during the development of atherosclerosis. Methods:Rabbits were fed a 1% cholesterol chow for 12 weeks to induce atherosclerosis. Sermn NOx levels and the responsiveness of platelet to NO donor SNP were determined every 4 weeks during maintaining on a chow containing 1% cholesterol. The measurement of serum lipids and the examination of morphological feature and endothelial-dependent relaxation of aorta were performed after 12 weeks of cholesterol diet. Resu/ts.Cholesterol diet significantly increased sermn levels of cholesterol and LDL, caused a remarkable platelet hyperaggregability, and produced an evident endothelial dysfunction as indicated by the diminished vasorelaxation induced by acetylcholine and endothelial cell lesion as exhibited by scanning electron microscope examination. The percentage of inhibition of ADP-induced platelet aggregation by NO donor SNIP was significantly smaller in cholesterol chow group than that in normal chow group although no significant difference in serum NOx levels between normal and cholesterol chow group was observed throughout the development of atherosclexosis. :The present study suggests that the endothelial dysfunction caused by enhanced sermn cholesterol and LDL levels induces a decreased responsiveness of platelet to NO.

  7. Biology of three species of North American Triatominae (Hemiptera: Reduviidae: Triatominae fed on rabbits

    Directory of Open Access Journals (Sweden)

    José Alejandro Martínez-Ibarra

    2007-12-01

    Full Text Available Aspects related to hatching, lifetime, number of blood meals for molting, mortality, feeding time, and postfeeding defecation delay were evaluated and compared in each instar of three North American Triatominae: Triatoma gerstaeckeri, Triatoma lecticularia and Triatoma protracta, all of them fed on rabbits. No significant differences (p > 0.05 were found among the three species regarding mean hatching rate, which was close to 20 days. Egg-to-adult development times were significantly shorter (p < 0.05 for T. lecticularia. Number of blood meals for molting to next instar ranged from one to five for T. protracta, and from one to six for T. gerstaeckeri and T. lecticularia. Mortality rates were higher in younger nymphs of T. lecticularia and T. protracta, while rates in T. gerstaeckeri were higher in fifth-instar nymphs. Mean feeding time was longest in T. gerstaeckeri, followed by T. lecticularia. More than twice the number of T. gerstaeckeri nymphs completed the development process, if compared to the nymphs from the other two species. Defecation delay was less than 10 min for T. lecticularia, T. protracta and the youngest nymphs of T. gerstaeckeri. Results point out that these three species may be important potential vectors of Trypanosoma cruzi for human populations, in areas of Mexico where these species are currently present.

  8. Anti-atherosclerotic effects of garlic preparation in freeze injury model of atherosclerosis in cholesterol-fed rabbits.

    Science.gov (United States)

    Sobenin, Igor A; Andrianova, Irina V; Lakunin, Konstantin Y; Karagodin, Vasilii P; Bobryshev, Yuri V; Orekhov, Alexander N

    2016-10-15

    Garlic (Allium sativum L.) is one of the most popular substances used to reduce various risks associated with cardiovascular disease. However, little is known on the direct effects of garlic on atherosclerosis. In the present study we have examined the effect of per oral administration of the time-released garlic herbal preparation on serum atherogenicity and formation of intimal thickening after freeze injury in cholesterol-fed rabbits. Group 1 rabbits maintained on the standard cholesterol-rich diet served as the control. Group 2 rabbits were fed the cholesterol-rich diet and treated with garlic preparation containing 300 mg garlic powder. Local thickening of the aortic media (i.e., the neointima formation) in the freeze injury zone was observed in all the rabbits. Regular garlic preparation therapy prevented the neointima formation and the accumulation of free and esterified cholesterol, triglycerides, phospholipids and collagen in the neointima, the effects being statistically significant. Garlic preparation also decreased serum lipid content by 1.5-fold and lowered atherogenic activity of blood serum (ability to induce lipid accumulation in cultured cells) induced by cholesterol-rich diet. The results obtained indicate that garlic preparation prevents the development of cholesterol-induced experimental atherosclerosis and possesses the direct anti-atherogenic activity. Copyright © 2015 Elsevier GmbH. All rights reserved.

  9. Haematology, Blood Chemistry and Carcass Characteristics of Growing Rabbits Fed Grasshopper Meal as a Substitute for Fish Meal

    Directory of Open Access Journals (Sweden)

    A. A. Njidda* and C. E. Isidahomen1

    2010-01-01

    Full Text Available An experiment was conducted to evaluate the effect of replacing fish meal with grasshopper meal on haematology, blood chemistry and carcass characteristics of growing rabbits. Forty rabbits of mixed breeds, aged 6-10 weeks, were randomly assigned to the dietary treatments in a complete randomized design with eight rabbits per treatment. The rabbits were fed with diets containing 0, 1.25, 2.50, 3.75 and 5% grasshopper meal in diets designated as T1 (control, T2, T3, T4 and T5, respectively. The experimental diets and clean drinking water were supplied ad libitum throughout the experimental period of nine weeks. At the end of the feeding trial, three rabbits per treatment were slaughtered for carcass evaluation, while blood samples were collected for analysis. The result of the experiment showed significant differences (P0.05 on haemoglobin and mean corpuscular haemoglobin concentration (MCHC. The results also revealed significant differences (P0.05 on serum albumin and total protein. The results of carcass characteristics showed significant differences among treatments (P<0.05 for slaughter weight, carcass weight, dressing percentage, skin pelt, tail, feet and abdominal fat. The slaughter weight and carcass weight were better in groups receiving 2.5% grass hopper meal (50% fish meal replacement. From the results, it can be concluded that inclusion of 2.50% grasshopper meal as a replacement for fish meal (50% replacement has no adverse effects on the haematological parameters, serum biochemistry and carcass characteristics of rabbits.

  10. Growth rate of rex, satin rabbits and their cross fed with lactosym@ in an intensive raising system

    Directory of Open Access Journals (Sweden)

    Djamura Aritonang

    2003-10-01

    Full Text Available An experiment was conducted to study the effect of breed, level of lactosym and their interaction on the growth performance of three breeds of rabbit, i.e.: Rex, Satin and their Cross. Thirty three-six week old (weaning rabbits were contribute in a 3 x 3 factorial used of completely randomized design having 3-5 individual replication and fed one out of three levels of lactosym (0, 0.25 and 0.50 ml/head/week. The two factors were: breed and level of lactosym. Result showed that there were no significant differences between breed (P>0,05 for body weight gain, feed intake and feed conversion. Body weight was affected by the level of lactosym (P0,05. Interaction between breed and level of lactosym did not affect body weight gain and feed conversion (P>0,05, but affected feed intake (P<0,05.

  11. Performance of growing rabbits fed increasing levels of discarded Salvia hispanica L. (chia) seed.

    Science.gov (United States)

    Rodríguez-Abello, Diana; Ramírez-Avilés, Luis; Navarro-Alberto, Jorge; Zamora-Bustillos, Roberto

    2016-06-01

    This experiment was carried out to evaluate the effect of including five levels of discarded chia seed (DCS) on growth, energy, and economic efficiency in rabbits. Fifty crossbred (New Zealand × Californian) weaning rabbits, weighing 798 ± 170 g, were randomly assigned to the following treatments: 0 % (control), 10 % (Ch10), 20 % (Ch20), 30 % (Ch30), and 40 % (Ch40) of DCS. Ten unsexed rabbits were allocated to each treatment and housed individually in cages; the experimental period was 6 weeks. There were no statistically significant differences between treatments in final body weight (2050 g), body weight gain (30 g/rabbit/day), and carcass weight (1010 g); however, feed intake (91 g/rabbit/day in Ch20 vs. 80 g/rabbit/day in Ch40), feed conversion ratio (2.77 in control and Ch40 vs. 2.95 in Ch10, Ch20, and Ch30), energy conversion ratio (32 MJ/kg in Ch20 vs. 29 MJ/kg on average in control, Ch10, Ch20, Ch30, and Ch40), and economic efficiency (358 % in control vs. 433 % in Ch40) showed differences (P < 0.05) between treatments. Discarded chia seed can be included up to 40 % in growing rabbit diets without any adverse effects on growth and carcass weight, and increased economic efficiency.

  12. FEDS

    DEFF Research Database (Denmark)

    Venable, John; Pries-Heje, Jan; Baskerville, Richard

    2016-01-01

    to enable Design Science Researchers to effectively design and incorporate evaluation activities into a DSR project that can achieve DSR goals and objectives. To address this research gap, this research paper develops, explicates, and provides evidence for the utility of a Framework for Evaluation in Design...... Science (FEDS) together with a process to guide design science researchers in developing a strategy for evaluating the artefacts they develop within a DSR project. A FEDS strategy considers why, when, how, and what to evaluate. FEDS includes a two-dimensional characterisation of DSR evaluation episodes...... on an actual DSR project....

  13. Evaluation of the reproductive performance of rabbits does fed a half-simplified diet based on cassava byproducts

    Directory of Open Access Journals (Sweden)

    Andréia Fróes Galuci Oliveira

    2011-11-01

    Full Text Available A total of 70 five-month-old female New Zealand White rabbits were assigned in a completely randomized design, over three reproductive cycles, with two treatments: a reference diet and a half-simplified diet containing 79.83% cassava byproduct. The study evaluated body weight and feed intake of does, feed cost, number and total body weight of kits at kindling and weaning per female during three cycles, number and percentage of mortality/female/cycle, and weight gain of kits from birth to weaning. No interaction was observed between the diets and among the reproductive cycles for any evaluated characteristics. The body weight of does at the moment of weaning was similar in both groups for all three reproductive cycles. However, does fed the half-simplified diet had lower feed intake during the three reproductive cycles and, consequently, more reproductive flaws. The number of kits at weaning, body weight of kits at kindling and weaning, weight gain of kits from birth to weaning, and total body weight of kits at weaning were lower for the group of does fed the half-simplified diet and, consequently, there was a higher number and percentage of dead kits in this group. The total numbers of kits at kindling and weaning and total body weight of kits at birth during all three reproductive cycles were similar between the groups; however, total body weight of weaning rabbits was higher for the animals receiving the reference diet. It is possible to conclude that although the use of the half-simplified diet decreases the reproductive performance of does, it reduces feed cost per kg of body weight by 23.63% compared with the reference diet, proving to be a viable nutritional option for rabbit production.

  14. On the anti-atherogenic effect of the antioxidant BHT in cholesterol-fed rabbits: inverse relation between serum triglycerides and atheromatous lesions.

    Science.gov (United States)

    Freyschuss, A; Al-Schurbaji, A; Björkhem, I; Babiker, A; Diczfalusy, U; Berglund, L; Henriksson, P

    2001-12-30

    We have shown that inclusion of the antioxidant butylated hydroxytoluene (BHT) in the diet protects against development of atherosclerotic lesions in cholesterol-fed rabbits. In parallel, BHT treatment results in increased plasma triglyceride levels. The present study explores the relationship between the triglyceride-inducing and protective effects of BHT in two different studies. The combined material contains 22 rabbits fed cholesterol and 18 rabbits fed cholesterol in combination with 1% BHT. In the BHT group there was an inverse relationship between triglyceride exposure/cholesterol exposure and extent of lesions with r=0.74 (P=0.0005). Our results show that increased triglyceride exposure parallels the anti-atherogenic effect of BHT. There was no significant correlation between atheromatosis and serum BHT levels. beta-very low density lipoprotein (beta-VLDL) from cholesterol and BHT animals was triglyceride-enriched and smaller compared to beta-VLDL from cholesterol-fed animals, but there was no significant association between the anti-atherogenic effect of BHT and particle size or apolipoprotein pattern of LDL or beta-VLDL. LDL isolated from rabbits treated with cholesterol and BHT was less sensitive to oxidative modification than LDL isolated from rabbits treated with cholesterol only. In conclusion, our results demonstrate that the degree of triglyceride exposure may be an important modulator of the anti-atherogenic effect of an antioxidant.

  15. FEDS

    DEFF Research Database (Denmark)

    Pries-Heje, Jan; Venable, John; Baskerville, Richard

    2016-01-01

    Evaluation of design artefacts and design theories is a key activity in Design Science Research (DSR), as it provides feedback for further development and (if done correctly) assures the rigour of the research. However, the extant DSR literature provides insufficient guidance on evaluation...... to enable Design Science Researchers to effectively design and incorporate evaluation activities into a DSR project that can achieve DSR goals and objectives. To address this research gap, this research paper develops, explicates, and provides evidence for the utility of a Framework for Evaluation in Design...... Science (FEDS) together with a process to guide design science researchers in developing a strategy for evaluating the artefacts they develop within a DSR project. A FEDS strategy considers why, when, how, and what to evaluate. FEDS includes a two-dimensional characterisation of DSR evaluation episodes...

  16. Application of Fourier transform infrared spectroscopic imaging to the study of effects of age and dietary l-arginine on aortic lesion composition in cholesterol-fed rabbits

    Science.gov (United States)

    Palombo, Francesca; Cremers, Stephanie G.; Weinberg, Peter D.; Kazarian, Sergei G.

    2009-01-01

    Diet-induced atherosclerotic lesions in the descending thoracic segment of rabbit aorta were analysed ex vivo by micro-attenuated total reflection (ATR)–Fourier transform infrared (FTIR) spectroscopic imaging. The distribution and chemical character of lipid deposits within the arterial wall near intercostal branch ostia were assessed in histological sections from immature and mature rabbits fed cholesterol with or without l-arginine supplements. Previous studies have shown that both these properties change with age in cholesterol-fed rabbits, putatively owing to changes in the synthesis of nitric oxide (NO) from l-arginine. Immature animals developed lesions at the downstream margin of the branch ostium, whereas lipid deposition was observed at the lateral margins in mature animals. Dietary l-arginine supplements had beneficial effects in mature rabbit aorta, with overall disappearance of the plaques; on the other hand, they caused only a slight decrease of the lipid load in lesions at the downstream margin of the ostium in immature rabbits. ATR–FTIR imaging enabled differences in the lipid to protein density ratio of atherosclerotic lesions caused by age and diet to be visualized. Lipid deposits in immature rabbits showed higher relative absorbance values of their characteristic spectral bands compared with those in immature l-arginine-fed rabbits and mature rabbits. The multivariate methods of principal component analysis (PCA) and factor analysis (FA) were employed, and relevant chemical and structural information were obtained. Two distinct protein constituents of the intima–media layer at different locations of the wall were identified using the method of FA. This approach provides a valuable means of investigating the structure and chemistry of complex heterogeneous systems. It has potential for in vivo diagnosis of pathology. PMID:18986964

  17. Antihyperlipidemic Effect of Syrian Mesquite (Prosopis farcta) Root in High Cholesterol Diet-Fed Rabbits.

    Science.gov (United States)

    Saidi, Mohammad Reza; Farzaei, Mohammad Hosein; Miraghaee, Shahram; Babaei, Atefeh; Mohammadi, Bahareh; Bahrami, Mohammad Taher; Bahrami, Gholamreza

    2016-10-01

    Prosopis farcta root has been proposed as an efficacious natural drug for cardiovascular disorders in traditional medicine. The present study evaluates the efficacy of aqueous extract of Prosopis farcta root on experimental atherosclerosis development in rabbits with high cholesterol diet-induced hypercholesterolemia. Serum lipid parameters were significantly increased in the high cholesterol diet groups in comparison with the normal control group (P Prosopis farcta root significantly reduced total cholesterol, triglyceride, high-density lipoprotein, low-density lipoprotein, and very low density lipoprotein levels compared to high cholesterol diet rabbits (P < .050). This finding may reflect a reduction of chest pain or the beneficial effects of this plant root extract on cardiovascular health. The present study can serve as a basis for future investigations on the other effects of this plant on cardiovascular health.

  18. Early extracellular and cellular lipid deposits in aorta of cholesterol-fed rabbits.

    OpenAIRE

    Guyton, J. R.; Klemp, K. F.

    1992-01-01

    Subendothelial accumulation of extracellular liposomes rich in unesterified cholesterol has been described as an early feature of atherosclerosis induced by cholesterol feeding in rabbits. Beta-very-low-density lipoproteins, however, the presumed source of atherogenic lipid in this animal model, contain mostly esterified cholesterol. The purpose of this study was to test for the presence of extracellular neutral lipid deposits consistent with esterified cholesterol, by employing new electron ...

  19. Early extracellular and cellular lipid deposits in aorta of cholesterol-fed rabbits.

    OpenAIRE

    Guyton, J. R.; Klemp, K. F.

    1992-01-01

    Subendothelial accumulation of extracellular liposomes rich in unesterified cholesterol has been described as an early feature of atherosclerosis induced by cholesterol feeding in rabbits. Beta-very-low-density lipoproteins, however, the presumed source of atherogenic lipid in this animal model, contain mostly esterified cholesterol. The purpose of this study was to test for the presence of extracellular neutral lipid deposits consistent with esterified cholesterol, by employing new electron ...

  20. Estimation of humoral immune response in rabbits fed with Cucurbita maxima seeds

    Directory of Open Access Journals (Sweden)

    V. Ranganathan

    Full Text Available Aim : The objective of the study was to estimate the humoral immune response in rabbits treated with Cucurbita maxima seeds. Materials and Methods: Thirty six male Newzealand White rabbits were divided into six groups (I, II, III, IV, V, VI of six in each. Group I was the untreated control. Group II was treated with dexamethasone sodium (2 mg/Kg, i.m for 7 days. Group III was treated with levamisole hydrochloride at 2.5 mg/kg (s.c thrice a week. Group IV was treated with Cucurbita maxima seeds. Group V was treated with levamisole and dexamethasone and Group VI was treated with dexamethasone and Cucurbita maxima seeds. The seed was given @ 1000 mg/kg orally for 10 days. Antibody titre and serum immunoglobulin concentration were estimated along with haematology. Results: Dexamethasone caused significant decreases in the antibody titre, immunoglobulin concentration where as Curcurbita maxima, Dexamethasone + Curcurbita maxima and dexamethasone + levamisole groups showed significant increase in these entities. There were no significant differences in RBC count, Haemoglobin contents among all the groups studied. Conclusion: Results suggest that Cucurbita maxima seeds has the ability to stimulate humoral immune response in rabbits. [Vet World 2013; 6(7.000: 396-399

  1. Antiatherosclerotic Effect of Canarium odontophyllum Miq. Fruit Parts in Rabbits Fed High Cholesterol Diet

    Science.gov (United States)

    Shakirin, Faridah Hanim; Azlan, Azrina; Ismail, Amin; Amom, Zulkhairi; Yuon, Lau Cheng

    2012-01-01

    The effect of C. odontophyllum (CO) fruit parts was investigated in hypercholesterolemic rabbits. Forty-nine rabbits, which were randomly divided into seven groups of seven animals (n = 7), received a diet containing different parts of CO fruit parts for 8 weeks. The groups were as follows: (1) normal diet: NC group and (2) hypercholesterolemic diet: PC, HS (10 mg/kg/day simvastatin), HPO (20 g kg−1 oil extracted from the pulp of CO), HKO (20 g kg−1 oil extracted from the kernel of CO), HF (50 g kg−1 fullfat pulp of CO), and HD (50 g kg−1 defatted pulp of CO). Among these groups, rabbits receiving defatted pulp of CO showed the greatest cholesterol lowering effect as it had reduced plasma LDL-C, TC, and thiobarbiturate reactive substance (TBARS) levels as well as atherosclerotic plaques. The presence of high dietary fiber and antioxidants activity are potential factors contributing to the cholesterol lowering effect. Consequently, these results indicate the potential use of CO defatted pulp as a cholesterol lowering and antioxidant agent. PMID:22811751

  2. Antiatherosclerotic Effect of Canarium odontophyllum Miq. Fruit Parts in Rabbits Fed High Cholesterol Diet

    Directory of Open Access Journals (Sweden)

    Faridah Hanim Shakirin

    2012-01-01

    Full Text Available The effect of C. odontophyllum (CO fruit parts was investigated in hypercholesterolemic rabbits. Forty-nine rabbits, which were randomly divided into seven groups of seven animals (n=7, received a diet containing different parts of CO fruit parts for 8 weeks. The groups were as follows: (1 normal diet: NC group and (2 hypercholesterolemic diet: PC, HS (10 mg/kg/day simvastatin, HPO (20 g kg−1 oil extracted from the pulp of CO, HKO (20 g kg−1 oil extracted from the kernel of CO, HF (50 g kg−1 fullfat pulp of CO, and HD (50 g kg−1 defatted pulp of CO. Among these groups, rabbits receiving defatted pulp of CO showed the greatest cholesterol lowering effect as it had reduced plasma LDL-C, TC, and thiobarbiturate reactive substance (TBARS levels as well as atherosclerotic plaques. The presence of high dietary fiber and antioxidants activity are potential factors contributing to the cholesterol lowering effect. Consequently, these results indicate the potential use of CO defatted pulp as a cholesterol lowering and antioxidant agent.

  3. Quality and lipid composition of spermatozoa in rabbits fed DHA and vitamin E rich diets.

    Science.gov (United States)

    Gliozzi, T M; Zaniboni, L; Maldjian, A; Luzi, F; Maertens, L; Cerolini, S

    2009-04-01

    The effects of fish oil (FO) and vitamin E (vE) dietary supplementation on semen quality, sperm susceptibility to lipid peroxidation, tocopherols content and fatty acid profiles were studied in rabbits. Fifty-two rabbit bucks randomly divided in four groups received a control diet and enriched diets containing either FO (1.5%, w/w), vE (200mg/kg) or both. Semen volume, concentration, motility and viability were analysed at various time-points and the lipid composition was assessed on sperm cells. The phospholipid fatty acid profile was determined: n-6 PUFA were the major fatty acids found, with a proportion of 42%, whereas the n-3 PUFA accounted for nearly 1%, mainly represented by C22:6n-3 (docosahexaenoic acid, DHA). FO supplementation produced a seven-fold increase in the content of DHA in sperm phospholipids and a comprehensive rearrangement of the phospholipid fatty acid composition, while an unexpected negative effect of feeding high level of vE on the proportion of total PUFA was found. Despite the remarkable changes observed in sperm lipid composition, semen quality parameters were not affected by the dietary treatments and the interaction between the two dietary supplements had a significant effect only on sperm concentration. An increase in semen production by ageing and a concomitant rise in sperm susceptibility to in vitro peroxidation was found. alpha- and delta-tocopherol, present in rabbit sperm in similar amount, were not affected by dietary treatment. delta-tocopherol content had a significant linear negative regression with age and showed a significant negative correlation with the susceptibility to peroxidation values.

  4. [Effect of the brand and generic medicine of pravastatin on dyslipidemia in rabbits fed a high cholesterol diet].

    Science.gov (United States)

    Kano, Seiichiro; Taguchi, Mutsumi; Hayase, Nobumasa; Kaneta, Shigeru; Takaguri, Akira; Ichihara, Kazuo; Satoh, Kumi

    2009-01-01

    Mevalotin containing pravastatin, a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor, is the brand medicine and well known to be effective for patients with dyslipidemia. Now, more than 20 generic pravastatins are available for clinical therapy. We compared pharmaceutical property of Mevan,a generic pravastatin, with that of Mevalotin.According to the definition of the Japanese Pharmacopoeia, Mevalotin 10 mg tablets were uniform in pravastatin content, whereas 5 mg tablets were rather variable. Variation in pravastatin content of Mevan 5 mg tablets was the same as Mevalotin 5 mg, whereas that of 10 mg tablets was very variable. The plasma concentration of pravastatin in the normal rabbits continuously increased until 180 min after oral administration of 30 mg Mevan, whereas it increased in a biphasic pattern after 30 mg Mevalotin.All rabbits were fed 0.2% cholesterol diet throughout the experiment. After 8 weeks, oral administration of either Mevalotin or Mevan was started at the dose of 30 mg pravastatin/day for 16 weeks. After a transient increase for a few weeks, the plasma levels of total- and LDL-cholesterol gradually decreased in Mevalotingroup, whereas these levels did not significantly changed in Mevan group within 16 weeks. The level of HDL-cholesterol in Mevan group tended to increase but not in Mevalotin group. The triglyceride level in Mevan group changed as well as that in Mevalotin group until 10 weeks after administration, and then gradually increased. The present results suggest that pharmaceutical properties of Mevan are not always identical with those of Mevalotin.

  5. Spirulina platensis effects on the levels of total cholesterol, HDL and triacylglycerols in rabbits fed with a hypercholesterolemic diet

    Directory of Open Access Journals (Sweden)

    Luciane Maria Colla

    2008-04-01

    Full Text Available In this work, hypercholesterolemia was induced in rabbits by feeding them a high cholesterol diet (CD, 350 mg/d and the effects of supplementing this diet with 0.5 g/d Spirulina platensis was evaluated by measuring the levels of serum total-cholesterol (TC, triacylglycerols (TAG and high-density lipoprotein (HDL-cholesterol at the start of the experiment and after 30 d and 60 d. It was found that the levels of serum cholesterol decreased from 1,054±101 mg.dL-1 in the rabbits fed a CD without S. platensis to 516±163 mg.dL-1 to those fed with a high cholesterol diet supplemented with S. platensis (significant at p A microalga Spirulina é cultivada e comercializada no mundo devido a suas características nutricionais (elevada concentração de proteínas, em torno de 65%, vitaminas e sais minerais e ao seu potencial terapêutico no tratamento de inúmeras doenças, inclusive a hipercolesterolemia. Neste trabalho foi avaliada a inibição da hipercolesterolemia induzida em coelhos por uma dieta adicionada de colesterol (350 mg.d-1, pela suplementação de 0,5 g.dia-1 de biomassa de Spirulina platensis, sendo avaliados os níveis de colesterol total, triglicerídeos e HDL nos tempos de 0 d, 30 d e 60 d de tratamento. Os resultados indicaram que a adição de Spirulina platensis na dieta ocasionou decréscimo nos níveis de colesterol total de 1054±101 mg.dL-1 para 516±163 mg.dL-1 (p<0,0001, para os coelhos alimentados com a dieta colesterolêmica em comparação com os que receberam a dieta adicionada de Spirulina platensis. A adição de Spirulina a dieta colesterolêmica não ocasionou decréscimo significativo nos níveis de triglicerídios dos coelhos. Os valores de HDL aumentaram de 73±31 mg.dL-1 para 91,0±15,7 mg.dL-1, comparando-se os coelhos alimentados com a dieta colesterolêmica e os alimentados com a dieta adicionada de Spirulina, estatisticamente diferentes a um nível de significância maior que 0,1533.

  6. Endobronchial Watanabe Spigot Placement for a Secondary Pneumothorax.

    Science.gov (United States)

    Ueda, Yuichiro; Huang, Cheng-Long; Itotani, Ryo; Fukui, Motonari

    2015-07-01

    Although bevacizumab has several adverse effects, pneumothorax is rare. This is the first case of initial treatment using an Endobronchial Watanabe Spigot (EWS) for pneumothorax after bevacizumab-containing chemotherapy. A 56-year-old woman with recurrent breast cancer was treated with bevacizumab. Pneumothorax occurred 6 days after the last administration of bevacizumab. The pneumothorax failed to resolve after the chest tube drainage. This was because the bronchopleural fistula formed at the site of the subpleural metastatic lesion. Patient was in need of a surgical repair of the bronchopleural fistula, which could not be carried out due to the recent bevacizumab administration. After the insertion of the EWS, the air leak stopped immediately. A lobectomy was successfully performed for the recurrent pneumothorax and for the resection of the metastatic lung lesion; at a most appropriate duration since the chemotherapy. EWS is useful as the initial palliation of pneumothorax after the treatment with medication causing delayed wound healing.

  7. A longitudinal cross-over study of serum cholesterol and lipoproteins in rabbits fed on semipurified diets containing either casein or soybean protein

    NARCIS (Netherlands)

    Terpstra, A.H.M.; Woodward, C.J.H.; West, C.E.; Boven, van H.G.

    1982-01-01

    1. Two groups, each of six rabbits, were fed on semi-purified diets containing either 400 g casein or 400 g soya-bean protein/kg for 20 d and then the diets of the two groups were crossed-over. 2. Just before the cross-over, the serum cholesterol concentration (mean ± SE) was 3068 ± 592 and 800 ± 14

  8. Accumulation of low density lipoprotein associated cholesterol in calcifying vesicle fractions correlates with intimal thickening in thoracic aortas of juvenile rabbits fed a supplemental cholesterol diet

    Directory of Open Access Journals (Sweden)

    Culley Nathan C

    2006-10-01

    Full Text Available Abstract Background It has been shown that calcifying vesicles play an important role in aortic calcification and that cholesterol content in the isolated vesicle fraction is increased when rabbits are fed supplemental cholesterol diets. Whether lipoprotein-associated cholesterols and other lipids are also increased in the vesicle fraction and whether the increase correlates with atherosclerosis remain unknown. Results Fourteen juvenile male rabbits fed an atherogenic diet containing 0.5% cholesterol and 2% peanut oil for 3 months developed varying degrees of hypercholesterolemia and intimal thickening in the ascending thoracic aorta. The correlation between these two parameters was insignificant, and likely attributable to the use of small numbers of rabbits in this study. Despite this lack of correlation, we demonstrate that the accumulation of cholesterol in calcifying vesicle fractions obtained from the collagenase-digested aorta fragments correlates well with intimal thickening (r2 = 0.98, p Conclusion When limited numbers of rabbits are used, LDL-C accumulation in calcifying vesicle fractions is a better biomarker for atherosclerosis than LDL-C levels in the serum. The close association of LDL-C with calcifying vesicles may play an important role in atherosclerosis and calcification.

  9. Effects of exogenous enzymes, Lactobacillus acidophilus or their combination on feed performance response and carcass characteristics of rabbits fed sugarcane bagasse

    Institute of Scientific and Technical Information of China (English)

    Nahla A Abdel-Aziz; Mounir El-Adawy; Maria A Mariezcurrena-Berasain; Abdelfattah Z M Salem; Jaime Olivares-Prez; Ahmed E Kholif; Borhami E Borhami

    2015-01-01

    The aim of this study was to evaluate the effect of Lactobacil us acidophilus (LAC), exogenous enzymes of ZAD® (exogenous enzyme preparation) or their combination on feed conversion, and dressing of rabbits fed different treatments of sugarcane bagasse (SCB). Five rations were al otted randomly to ifve groups of New-Zealand White (NZW) rabbits (n=10) with initial live body weight of (838±42.4) g and 5 weeks of age. Rabbits were fed on diets with different sources of ifber as fol ows, (i) a control diet composed of 100%berseem hay and 0%SCB, (i ) 50%berseem hay and 50%untreated SCB (USCB), (i i) 50%berseem hay and 50%SCB treated with L. acidophilus (LAC), (iv) 50%berseem hay and 50%SCB treated with ZAD® (ZAD), and (v) 50%berseem hay and 50%SCB treated with a combination of LAC+ZAD® (LZ). Treatments of SCB with L. acidophilus, ZAD® and LAC+ZAD® had the highest feed conversion ratio than both USCB and control. The dressing per-centage of rabbits that fed the LAC and LZ diets was higher (P<0.05) compared with that in the other groups. Performance index (PI) for LAC group was improved (P<0.05) compared to that for the other groups;however, PI for USCB group was the lowest (P<0.05). It could be concluded that treating SCB with L. acidophilus, exogenous enzymes of ZAD® or their combi-nation improved feed conversion and performance with more positive effects with L. acidophilus than the other treatments.

  10. Fertility potential of rabbit bucks fed maize-based diets containing graded levels of fumonisin B1

    Directory of Open Access Journals (Sweden)

    Kingsley Idahor

    2012-07-01

    Full Text Available Fumonisin B1-contaminated maize-based diets have been reported to be mycotoxic in animal species, yet more validated data and biomarkers are needed. In this study, Fumonisin B1 (FB1 infected yellow-maize was used to formulate Diets 1, 2 and 3, containing 1,700, 1,800 and 1,900µg FB1/kg diet, respectively. Sixty sexually matured bucks and does were used, but only the bucks were fed the FB1-contaminated diets for eight weeks. At the end of the feeding trial, the treated bucks were mated to the dry does that were fed FB1-free yellow maize-based diet. Effects on testis and live weight, feed utilisation, conception rate, embryo development and spermatozoa production per gram testis were monitored. Results indicated significant depression (P<0.05 in feed intake, from 546.77±12.09g/week in Diet 1 with the lowest FB1 concentration, to 509.84±21.98g/week in Diet 3 with the highest FB1 concentration in the diets. Weight gain was drastically reduced (P<0.05 from 34.13±9.32g/week in Diet 1 to 20.38±22.13g/week in Diet 3. Meanwhile, some of the untreated does were pregnant in all the treatments, indicating that FB1 concentration at 2.0mg/kg diet may not be spermaticidal and there were no abnormalities in the embryos. It was observed that the paired testis weight value in Diet 1 (3.06±0.31 was not significantly different (P>0.05 from that of Diet 3 (2.94±0.23. The testicular elements were distorted by the dietary FB1, but did not follow a definite pattern. Spermatozoa production per gram testis values (31.1 – 37.3 x 106 recorded were much higher than 24 x 106 for a healthy rabbit buck. Consequently, FB1 concentration < 2.0mg/kg diet may not affect the fertility potentials of bucks orally dosed for a relatively short period. This observation further elucidates earlier discoveries that FB1 is not a reproductive toxicant. Hence, the United States Food and Drug Administration proposed a guideline of 2.0mg total fumonisins/kg diet, to be adopted with

  11. DISKRIMINASI GENDER DALAM NOVEL GINKO KARYA JUNICHI WATANABE

    Directory of Open Access Journals (Sweden)

    Linda Unsriana

    2015-04-01

    Full Text Available Discrimination is an attitude and behavior that violates human rights. Discrimination can also be interpreted as a treatment for individuals differently based on race, religion, or gender. Any harassment, restriction, or exclusion to race, religion, or gender includes discriminatory actions. The theme of discrimination experienced by many women made the writer want to examine it from literature point of view, especially Japanese literature. The problem in this paper is gender discrimination experienced by the main character in novel Ginko. In Ginko novel written by Junichi Watanabe, the theme of discrimination against women is very strong, as experienced by the main character named Gin Ogino. This study used feminist standpoint research with the assumption that gender discrimination in society in the novel cannot be separated from womens real experiences perceived by the author. In addition to stand on or derived from real experiences from the first woman doctor in Japan, which with all her efforts to break away from discrimination against women endured throughout her life: before marriage, divorce, attending medical school to become a doctor, even after she was graduated from medical school, she still experienced gender discrimination. This study found that gender discrimination experienced by Ginko because she is a woman, in which at that time (the Meiji era there were clear boundaries between men and women. Difficulty and discrimination experienced are because Ginkos ideals were considered impossible, because she wanted to become a doctor. Her ability and cleverness were obstructed just because she is a woman. It can be concluded in the Meiji era, there was discrimination against women reflected in the Novel Ginko.

  12. Diskriminasi Gender dalam Novel Ginko Karya Junichi Watanabe

    Directory of Open Access Journals (Sweden)

    Linda Unsriana

    2014-05-01

    Full Text Available Discrimination is an attitude and behavior that violates human rights. Discrimination can also be interpreted as a treatment for individuals differently based on race, religion, or gender. Any harassment, restriction, or exclusion to race, religion, or gender includes discriminatory actions. The theme of discrimination experienced by many women made the writer want to examine it from literature point of view, especially Japanese literature. The problem in this paper is gender discrimination experienced by the main character in novel Ginko. In Ginko novel written by Junichi Watanabe, the theme of discrimination against women is very strong, as experienced by the main character named Gin Ogino. This study used feminist standpoint research with the assumption that gender discrimination in society in the novel cannot be separated from women’s real experiences perceived by the author. In addition to stand on or derived from real experiences from the first woman doctor in Japan, which with all her efforts to break away from discrimination against women endured throughout her life: before marriage, divorce, attending medical school to become a doctor, even after she was graduated from medical school, she still experienced gender discrimination. This study found that gender discrimination experienced by Ginko because she is a woman, in which at that time (the Meiji era there were clear boundaries between men and women. Difficulty and discrimination experienced are because Ginko’s ideals were considered impossible, because she wanted to become a doctor. Her ability and cleverness were obstructed just because she is a woman. It can be concluded in the Meiji era, there was discrimination against women reflected in the Novel Ginko.

  13. Melatonin ameliorates vascular endothelial dysfunction, inflammation, and atherosclerosis by suppressing the TLR4/NF-κB system in high-fat-fed rabbits.

    Science.gov (United States)

    Hu, Ze-Ping; Fang, Xiao-Ling; Fang, Nan; Wang, Xiao-Bian; Qian, Hai-Yan; Cao, Zhong; Cheng, Yuan; Wang, Bang-Ning; Wang, Yuan

    2013-11-01

    Vascular endothelial dysfunction (VED) and inflammation contribute to the initiation and progression of atherosclerosis. Melatonin (MLT) normalizes lipid profile, improves endothelial function, and possesses anti-inflammatory properties. However, the precise mechanisms are still unclear. This study investigated whether MLT could ameliorate VED, inflammation, and atherosclerosis by suppressing the Toll-like receptor 4 (TLR4)/nuclear factor kappa B (NF-κB) system in high-fat-fed rabbits. Rabbits were randomly divided into three groups that received a standard diet (control group), high-cholesterol diet (atherosclerosis group), or high-cholesterol diet plus 10 mg/kg/day MLT (MLT group) for 12 wk. After treatment, high-fat diet significantly increased serum lipid and inflammatory markers in rabbits in atherosclerosis group compared with that in control group. In addition, high-fat diet also induced VED and typical atherosclerotic plaque formation and increased intima/media thickness ratio, which were significantly improved by MLT therapy as demonstrated in MLT group. Histological and immunoblot analysis further showed that high-fat diet enhanced the expressions of TLR4, myeloid differentiation primary response protein (MyD88), and NF-κB p65, but decreased inhibitor of NF-κB (IκB) expression. By contrast, MLT therapy decreased the expressions of TLR4, MyD88, and NF-κB p65 and increased IκB expression. This study has demonstrated that MLT ameliorates lipid metabolism, VED, and inflammation and inhibits the progression of atherosclerosis in high-fat-fed rabbits. Moreover, our study indicates for the first time that suppression of the TLR4/NF-κB system in local vasculature with atherosclerotic damage is important for the protective effects of MLT.

  14. Identification of N-terminally truncated pyroglutamate amyloid-β in cholesterol-enriched diet-fed rabbit and AD brain.

    Science.gov (United States)

    Perez-Garmendia, Roxanna; Hernandez-Zimbron, Luis Fernando; Morales, Miguel Angel; Luna-Muñoz, José; Mena, Raul; Nava-Catorce, Miriam; Acero, Gonzalo; Vasilevko, Vitaly; Viramontes-Pintos, Amparo; Cribbs, David H; Gevorkian, Goar

    2014-01-01

    The main amyloid-β peptide (Aβ) variants detected in the human brain are Aβ1-40 and Aβ1-42; however, a significant proportion of Aβ in Alzheimer's disease (AD) brain also consists of N-terminal truncated/modified species. AβN3(pE), Aβ peptide bearing amino-terminal pyroglutamate at position 3, has been demonstrated to be a major N-truncated/modified constituent of intracellular, extracellular, and vascular Aβ deposits in AD and Down syndrome brain tissue. It has been previously demonstrated that rabbits fed a diet enriched in cholesterol and given water containing trace copper levels developed AD-like pathology including intraneuronal and extracellular Aβ accumulation, tau hyperphosphorylation, vascular inflammation, astrocytosis, microgliosis, reduced levels of acetylcholine, as well as learning deficits and thus, may be used as a non-transgenic animal model of sporadic AD. In the present study, we have demonstrated for the first time the presence of AβN3(pE) in blood vessels in cholesterol-enriched diet-fed rabbit brain. In addition, we detected AβN3(pE) immunoreactivity in all postmortem AD brain samples studied. We believe that our results are potentially important for evaluation of novel therapeutic molecules/strategies targeting Aβ peptides in a suitable non-transgenic animal model.

  15. [Effect of electrostimulation "hunger center" of lateral hypothalamus on the impulse activity of masticatory muscles in unfed and fed rabbits in the absence and the presence of food].

    Science.gov (United States)

    Ignatova, Iu P; Kromin, A A

    2011-01-01

    In chronic experiences on rabbits the influence of electrostimulation of "the hunger centres" of the lateral hypothalamus (LH) on impulse activity of chewing muscles of rabbits under the conditions of hunger and satiation was studied. It is established, threshold irritation of the LH of hungry and preliminary fed animals in the absence of food caused the occurrence of incessant search behavior which was followed by the regular generation of bursts of AP by masseter and mylohyoideus muscles with bimodal distributions of interpulse intervals. Such reaction of chewing muscles during irritation of the LH in the absence of food is an example of advancing type reaction. The increase of level of alimentary motivation, arising at threshold irritation of the LH of rabbits under the conditions of hunger and satiety during the resultant food-intake behavior, increased frequency of generation of bursts of AP in a phase of the capture of food, but did not influence on this indicator in a phase of chewing of food. The received results testify about descending stimulating influences of alimentary motivational excitation on neurons of the chewing centre in medulla and on impulse activity of chewing muscles.

  16. Fertility, mortality, milk output, and body thermoregulation of growing Hy-Plus rabbits fed on diets supplemented with multi-enzymes preparation.

    Science.gov (United States)

    Gado, Hany M; Kholif, Ahmed E; Salem, Abdelfattah Z M; Elghandour, Mona M M; Olafadehan, Oluwarotimi A; Martinez, Maricela A; Al-Momani, Ahmed Q

    2016-10-01

    The aim of this study was to evaluate the fertility status, milk output, mortality, and body thermoregulation of rabbit does as affected by different levels of multi-enzyme extracts (EZ) in their diets. A total of 120 Hy-Plus rabbit does were divided into four comparable experimental groups (n = 30 does per group). Animals of each group were divided in six pens (five animals per pen), and each pen was used as an experimental unit. The first group was kept untreated and fed a commercial diet alone without enzyme extracts (EZ0), while the other groups were fed the same diet but supplemented with 1 (EZ1), 3 (EZ3), and 5 (EZ5) kg/ton of enzyme extracts, respectively. Feeding EZ additive increased (P < 0.05) conception and kindling rates, litter size and weight at birth, and litter size and bunny weight at weaning, with decreasing (P < 0.05) abortion rate. Moreover, total milk yield increased (P < 0.05) with increasing level of enzyme supplementation. Pre-weaning mortality decreased (P < 0.05) with EZ inclusion. Signs of vitality (rectal temperature, skin temperature, earlobe temperature, respiration rate, and pulse rate) were improved with EZ inclusion. For all results, 5 kg EZ/ton of feed was more effective than 1 and 3 kg EZ/ton feed. It can be concluded that supplementation of EZ in rabbit diet decreased mortality rate and enhanced fertility status and milk output.

  17. The Arginine/ADMA Ratio Is Related to the Prevention of Atherosclerotic Plaques in Hypercholesterolemic Rabbits When Giving a Combined Therapy with Atorvastatine and Arginine

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    Saskia J. H. Brinkmann

    2015-05-01

    Full Text Available Supplementation with arginine in combination with atorvastatin is more efficient in reducing the size of an atherosclerotic plaque than treatment with a statin or arginine alone in homozygous Watanabe heritable hyperlipidemic (WHHL rabbits. We evaluated the mechanism behind this feature by exploring the role of the arginine/asymmetric dimethylarginine (ADMA ratio, which is the substrate and inhibitor of nitric oxide synthase (NOS and thereby nitric oxide (NO, respectively. Methods: Rabbits were fed either an arginine diet (group A, n = 9, standard rabbit chow plus atorvastatin (group S, n = 8, standard rabbit chow plus an arginine diet with atorvastatin (group SA, n = 8 or standard rabbit chow (group C, n = 9 as control. Blood was sampled and the aorta was harvested for topographic and histological analysis. Plasma levels of arginine, ADMA, cholesterol and nitric oxide were determined and the arginine/ADMA ratio was calculated. Results: The decrease in ADMA levels over time was significantly correlated to fewer aortic lesions in the distal aorta and total aorta. The arginine/ADMA ratio was correlated to cholesterol levels and decrease in cholesterol levels over time in the SA group. A lower arginine/ADMA ratio was significantly correlated to lower NO levels in the S and C group. Discussion: A balance between arginine and ADMA is an important indicator in the prevention of the development of atherosclerotic plaques.

  18. Effects of Dietary Fatty Acids on Lipid Traits in the Muscle and Perirenal Fat of Growing Rabbits Fed Mixed Diets.

    Science.gov (United States)

    Peiretti, Pier Giorgio

    2012-02-22

    The aim of this study was to evaluate the effects of various raw materials (spirulina, curcuma, tomato pomace, false flax, linseed, chia, perilla seeds) as suitable polyunsaturated fatty acid n-3 (n-3 PUFA) sources, on the lipid traits in the longissimus dorsi muscle and perirenal fat of growing rabbits. The fatty acid (FA) analyses of the diets, carried out by gas chromatography, differed over a wide range on the basis of the highly varied ingredients in 27 experimental formulations. Among the 29 identified FAs, three from feeds were catabolized in the rabbits, five were de novo synthesized and stored chiefly in the muscle. It was possible to linearly characterize the incorporation from the feed to the muscle of 16 FAs. This study has confirmed that the dietary inclusion of various raw materials could be considered as a way of enriching the n-3 PUFA of rabbit meat. A proposal for the prediction of n-3 PUFA from dietary α-linolenic acid (C18:3 n-3) and a panel of another 10 FAs has been made for intramuscular fat (R² = 0.94) and perirenal fat (R² = 0.96).

  19. Effects of Dietary Fatty Acids on Lipid Traits in the Muscle and Perirenal Fat of Growing Rabbits Fed Mixed Diets

    Directory of Open Access Journals (Sweden)

    Pier Giorgio Peiretti

    2012-02-01

    Full Text Available The aim of this study was to evaluate the effects of various raw materials (spirulina, curcuma, tomato pomace, false flax, linseed, chia, perilla seeds as suitable polyunsaturated fatty acid n-3 (n-3 PUFA sources, on the lipid traits in the longissimus dorsi muscle and perirenal fat of growing rabbits. The fatty acid (FA analyses of the diets, carried out by gas chromatography, differed over a wide range on the basis of the highly varied ingredients in 27 experimental formulations. Among the 29 identified FAs, three from feeds were catabolized in the rabbits, five were de novo synthesized and stored chiefly in the muscle. It was possible to linearly characterize the incorporation from the feed to the muscle of 16 FAs. This study has confirmed that the dietary inclusion of various raw materials could be considered as a way of enriching the n-3 PUFA of rabbit meat. A proposal for the prediction of n-3 PUFA from dietary α-linolenic acid (C18:3 n-3 and a panel of another 10 FAs has been made for intramuscular fat (R2 = 0.94 and perirenal fat (R2 = 0.96.

  20. Effect of chlorpromazine on lipid metabolism in aortas from cholesterol-fed rabbits and normal rats, in vitro: inhibition of sterol esterification and modification of phospholipid synthesis

    Energy Technology Data Exchange (ETDEWEB)

    Bell, F.P.

    1983-06-01

    Chlorpromazine (CPZ), a major tranquilizer, was found to be a potent inhibitor of acylCoA:cholesterol acyltransferase (ACAT, EC 2.3.1.26) in isolated arterial microsomes and in intact arterial tissue from the rat and cholesterol-fed rabbit in vitro. In isolated rabbit arterial microsomes, CPZ resulted in a concentration-dependent inhibition of ACAT with 50% inhibition of (1-14C)oleoylCoA incorporation into (14C)cholesteryl esters occurring at 0.1 mM CPZ. CPZ also effectively inhibited the incorporation of (14C)oleate into triglycerides without affecting incorporation into diglycerides. Additionally, CPZ altered the pattern of arterial phospholipids synthesized from (1-14C)oleate. Incorporation into phosphatidylcholine was depressed while incorporation into phosphatidylinositol was increased. Since diglyceride synthesis appeared to be unaffected by CPZ, a redirection of phosphatidic acid into the CDP-diglyceride pathway of glycerolipid synthesis does not adequately account for the effect of CPZ on arterial phospholipid and triglyceride synthesis in these experiments.

  1. NIRS of body and tissues in growing rabbits fed diets with different fat sources and supplemented with Curcuma longa

    Directory of Open Access Journals (Sweden)

    Pier Giorgio Peiretti

    2013-06-01

    Full Text Available A portable Near Infrared Reflectance Spectroscopy (NIRS instrument was applied to 40 growing rabbits to determine body and tissue differences induced by experimental factors. The rabbits were examined at 2 live sites, in 7 warm carcass tissues and in longissimus dorsi muscle samples prepared in ethanol. For this purpose, the method was applied in a bi-factorial experiment concerning the dietary oil source (O (maize vs. palm oil and Curcuma longa (C supplementation (0 and 3 g/kg, respectively. Significant chemical differences emerged for palmitic, oleic and linoleic acids in the longissimus dorsi muscle due to the O factor and for linolenic acid due to the C factor. The NIRS spectra and chemical analyses were elaborated by the Partial Least Squares (PLS method, and the rsquares in cross-validation (R2cv were retained as measure of the unoriented differentiation between the levels of the planned factor for each landmark and fatty acid (FA profile. Multivariate PLS analysis of the FA muscular fat showed that the O factor induced strong differentiation (R2cv: 0.96, while less influence (0.33 was observed for the C factor. The model based on the NIRS radiation of the landmarks clearly shows the O factor effects, not only in the perirenal (0.90 and scapular (0.85 fats, but also in the belly (0.76, liver (0.73 and hind legs (0.72. Whereas the C effects were only expressed in the live animals (ears: 0.66 and abdominal wall: 0.58 and in post-mortem (liver: 0.60. It was concluded that a preliminary NIRS scan of the carcass and of live rabbits can point out the presence of intrinsic experimental effects concerning the lipid metabolism of polyunsaturated FA of the n-6 series (O factor and n-3 series (C factor.

  2. Plasma and Aorta Biochemistry and MMPs Activities in Female Rabbit Fed Methionine Enriched Diet and Their Offspring

    Science.gov (United States)

    Aouichat Bouguerra, Souhila; Benazzoug, Yasmina

    2017-01-01

    This study investigated whether a high Met diet influences biochemical parameters, MMPs activities in plasma, and biochemical and histological remodeling in aorta, in both pregnant female rabbits and their offspring. Four female rabbit groups are constituted (each n = 8), nonpregnant control (NPC), pregnant control (PC) that received normal commercial chow, nonpregnant Met (NPMet), and pregnant Met (PMet) that received the same diet supplemented with 0,35% L-methionine (w/w) for 3 months (500 mg/d). All pregnant females realize 3 successive pregnancies. Plasma results showed that Met excess increased Hcy, raised CRP in NPMet and decreased it in PMet, enhanced significantly proMMP-2 and proMMP-9 activities in NPMet, and reduced them in PMet. Aorta showed a rise in collagen level, essentially in PMet, a reduction of elastin content in both PMet and NPMet, and a significant decrease in lipid content in PMet, with histological changes that are more pronounced in NPMet than PMet. Met excess enhanced proMMP-9 activities in NPMet while it decreased them in PMet. PMet newborn presented increase in uremia and CRP and significant rise of active MMP-2 and MMP-9 forms. In aorta, media and adventitia thickness increased, total lipids content decreased, proMMP-9 activity decreased, and proMMP-2 activity increased. PMID:28133545

  3. Plasma and Aorta Biochemistry and MMPs Activities in Female Rabbit Fed Methionine Enriched Diet and Their Offspring

    Directory of Open Access Journals (Sweden)

    Khira Othmani Mecif

    2017-01-01

    Full Text Available This study investigated whether a high Met diet influences biochemical parameters, MMPs activities in plasma, and biochemical and histological remodeling in aorta, in both pregnant female rabbits and their offspring. Four female rabbit groups are constituted (each n=8, nonpregnant control (NPC, pregnant control (PC that received normal commercial chow, nonpregnant Met (NPMet, and pregnant Met (PMet that received the same diet supplemented with 0,35% L-methionine (w/w for 3 months (500 mg/d. All pregnant females realize 3 successive pregnancies. Plasma results showed that Met excess increased Hcy, raised CRP in NPMet and decreased it in PMet, enhanced significantly proMMP-2 and proMMP-9 activities in NPMet, and reduced them in PMet. Aorta showed a rise in collagen level, essentially in PMet, a reduction of elastin content in both PMet and NPMet, and a significant decrease in lipid content in PMet, with histological changes that are more pronounced in NPMet than PMet. Met excess enhanced proMMP-9 activities in NPMet while it decreased them in PMet. PMet newborn presented increase in uremia and CRP and significant rise of active MMP-2 and MMP-9 forms. In aorta, media and adventitia thickness increased, total lipids content decreased, proMMP-9 activity decreased, and proMMP-2 activity increased.

  4. Effects of Inclusion of Two Probiotic Strains Isolated From “Sha’a”, a Maize-Based Traditionally Fermented Beverage on Lipid Metabolism of Rabbits fed a Cholesterol-Enriched Diet

    OpenAIRE

    Zambou Ngoufack François; Kaktcham Pierre Marie; Fonteh Anyangwe Florence; Guetiya Wadoum Raoul; Sieladie Djomne Victor

    2013-01-01

    The objective of this study was to assess the in vitro probiotic potential of Lactobacillus plantarum Lp10S and Lp11S isolated from Sha’a and evaluate their effects on lipid metabolism of hypercholesterolaemic rabbits. The strains were tested in vitro for their acid and bile tolerance, capability to remove cholesterol from MRS broth and to deconjugate bile salts. For in vivo studies, Lactobacillus plantarum Lp10S and Lp11S were administered to rabbits fed on cholesterol-enriched diet (standar...

  5. Effects of Curcuma comosa on the expression of atherosclerosis-related cytokine genes in rabbits fed a high-cholesterol diet.

    Science.gov (United States)

    Charoenwanthanang, Puttavee; Lawanprasert, Somsong; Phivthong-Ngam, Laddawal; Piyachaturawat, Pawinee; Sanvarinda, Yupin; Porntadavity, Sureerut

    2011-04-12

    Curcuma comosa has been known to have potential use in cardiovascular diseases, but its immunoregulatory role in atherosclerosis development and liver toxicity has not been well studied. We therefore investigated the effects of Curcuma comosa on the expression of atherosclerosis-related cytokine genes in rabbits fed a high-cholesterol diet. Twelve male New Zealand White rabbits were treated with 1.0% cholesterol for one month and were subsequently treated with 0.5% cholesterol either alone, or in combination with 5mg/kg/day of simvastatin or with 400mg/kg/day of Curcuma comosa powder for three months. The expression of IL-1, MCP-1, TNF-α, IL-10, and TGF-β in the isolated abdominal aorta and liver were determined by real-time RT-PCR. Liver toxicity was determined by hepatic enzyme activity. Curcuma comosa significantly decreased the expression of pro-inflammatory cytokines, leading to a stronger reduction in IL-1, MCP-1, and TNF-α expression compared to that was suppressed by simvastatin treatment. However, neither Curcuma comosa nor simvastatin affected the expression of anti-inflammation cytokines. In the liver, Curcuma comosa insignificantly decreased the expression of pro-inflammatory cytokines and significantly increased the expression of the anti-inflammatory cytokine IL-10 without altering the activity of hepatic enzymes. In contrast, simvastatin significantly increased the MCP-1 and TNF-α expressions and serum ALT level, without affecting the expression of anti-inflammatory cytokines. In this study, we demonstrated that Curcuma comosa exerts anti-inflammatory activity in the aorta and liver without causing liver toxicity, indicating that Curcuma comosa is a potential candidate as an alternative agent in cardiovascular disease therapy. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  6. Short time kernel asymptotics for Young SDE by means of Watanabe distribution theory

    CERN Document Server

    Inahama, Yuzuru

    2011-01-01

    In this paper we study short time asymptotics of a density function of the solution of a stochastic differential equation driven by fractional Brownian motion with Hurst parameter $H \\in (1/2, 1)$ when the coefficient vector fields satisfy an ellipticity condition at the starting point. We prove both on-diagonal and off-diagonal asymptotics under mild additional assumptions. Our main tool is Malliavin calculus, in particular, Watanabe's theory of generalized Wiener functionals.

  7. A water-extract of the Korean traditional formulation Geiji-Bokryung-Hwan reduces atherosclerosis and hypercholesteremia in cholesterol-fed rabbits.

    Science.gov (United States)

    Kim, Beob-Jin; Kim, Yeon-Kye; Park, Won-Hwan; Ko, Jeong-Heon; Lee, Young-Choon; Kim, Cheorl-Ho

    2003-05-01

    Geiji-Bokryung-Hwan (GBH), a drug preparation consisting of five herbs of Cinnamomi Ramulus (Geiji), Poria Cocos (Bokryun), Mountan Cortex Radicis (Mokdanpi), Paeoniae Radix (Jakyak) and Persicae Semen (Doin), is a traditional Korean herbal medicine that is widely used in the treatment of atherosclerosis-related disorders. A water extract of GBH was found to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and inhibit low-density lipoprotein (LDL) oxidation more effectively than probucol, a well-known commercially available antioxidant. In order to evaluate the anti-atherogenic potential of this medication, New Zealand White (NZW) rabbits were fed a normal diet for 12 weeks, a high cholesterol diet, a high cholesterol diet containing 1% probucol or a high cholesterol diet containing 5% water-soluble extract of GBH. Both GBH and probucol reduced plasma cholesterol levels. LDLs from the GBH-treated group were more resistant to Cu(2+)-induced oxidation and contained more vitamin E than LDLs from the high cholesterol diet group. Endothelial damage, determined at week 6, was reduced by 55% in the GBH group (P<0.01). GBH treatment reduced an atherosclerotic area in the abdominal aorta by 58% (P<0.05) and cholesterol deposition in the thoracic aorta by 55% (P<0.05). The severity of atherosclerosis in the GBH group was significantly reduced after an adjustment using cholesterol exposure as an index of the cholesterol-lowering effect. On the other hand, diet-induced hyperlipidemic rabbits were given water extract of GBH in doses of 50 (Group B) and 200 mg/kg (Group C) and compared with controls (Group A). At 40 days after intervention in groups A, B and C, total and LDL cholesterol levels were significantly lowered (P<0.01). LDL/high density lipoprotein (HDL) ratio was also significantly decreased (P<0.01). This study concludes that the reduction in atherosclerosis by GBH relies not only on its cholesterol-lowering effect but also more heavily on its antioxidant

  8. Reduction of atherosclerosis in cholesterol-fed rabbits and decrease of expressions of intracellular adhesion molecule-1 and vascular endothelial growth factor in foam cells by a water-soluble fraction of Polygonum multiflorum.

    Science.gov (United States)

    Yang, Peng-Yuan; Almofti, Mohamad Radwan; Lu, Ling; Kang, Hui; Zhang, Jing; Li, Tie-Jun; Rui, Yao-Cheng; Sun, Lian-Na; Chen, Wan-Sheng

    2005-11-01

    Polygonum multiflorum stilbeneglycoside (PMS) is a water-soluble fraction of Polygonum multiflorum Thunb., one of the most famous tonic traditional Chinese medicines, that has protective effects on the cardiovascular system. The purpose of the present study is to elucidate the effects of PMS on macrophage-derived foam cell functions and the reduction of severity of atherosclerosis in hypercholesterolemic New Zealand White (NZW) rabbits. NZW rabbits were fed for 12 weeks with a normal diet, a high cholesterol diet, or a high cholesterol diet associated with irrigation with different doses of PMS (25, 50, or 100 mg/kg). Treatment of NZW rabbits fed with high cholesterol diet with 100 mg/kg PMS attenuated the increase in plasma cholesterol, low-density lipoprotein cholesterol, very low-density lipoprotein cholesterol, and plasma triglyceride. Treatment with 50 and 100 mg/kg PMS caused 43% and 60% decrease in atherosclerotic lesioned area ratio to total surface area, respectively. In U937 foam cells, PMS could decrease the high expression of intercellular adhesion molecule (ICAM)-1 protein and the vascular endothelial growth factor (VEGF) protein levels in the medium induced by oxidized lipoprotein when analyzed by flow cytometry. The results proved that PMS is a powerful agent against atherosclerosis and that PMS action could possibly be through the inhibition of the expression of ICAM-1 and VEGF in foam cells.

  9. Changes in the vascular cell adhesion molecule-1, intercellular adhesion molecule-1 and c-reactive protein following administration of aqueous extract of piper sarmentosum on experimental rabbits fed with cholesterol diet

    Directory of Open Access Journals (Sweden)

    Al-Mekhlafi Hesham M

    2011-01-01

    Full Text Available Abstract Background Inflammation process plays an important role in the development of atherosclerosis. Hypercholesterolemia is one of the major risk factors for atherosclerosis. The present study aimed to evaluate the effect of aqueous extract of Piper sarmentosum (P.s on inflammatory markers like vascular cell adhesion molecule-1 (VCAM-1, intercellular adhesion molecule-1 (ICAM-1, and C-reactive protein (CRP. Methods Forty two male New Zealand white rabbits were divided equally into seven groups; (i C- control group fed normal rabbit chow (ii CH- cholesterol diet (1%cholesterol (iii X1- 1% cholesterol with water extract of P.s (62.5 mg/kg (iv X2- 1% cholesterol with water extract of P.s (125 mg/kg (v X3- 1% cholesterol with water extract of P.s (250 mg/kg (vi X4- 1% cholesterol with water extract of P.s (500 mg/kg and (vii SMV group fed with 1% cholesterol supplemented with simvistatin drug (1.2 mg/kg. All animals were treated for 10 weeks. Blood serum was taken for observing the inflammatory markers at the beginning and end of the experiment. Results Rabbits fed with 1% cholesterol diet (CH showed significant increase in the level of VCAM-1, ICAM-1 and CRP compared to the C group. The levels of VCAM-1, ICAM-1 and CRP in the 1% cholesterol group and supplemented with P.s (500 mg/kg were significantly reduced compared to the cholesterol group. Similar results were also reported with simvistatin group. Conclusion These results suggest that the supplementation of Piper sarmentosum extract could inhibit inflammatory markers which in turn could prevent atherosclerosis.

  10. Successful Treatment of Bronchial Fistula after Pulmonary Lobectomy by Endobronchial Embolization Using an Endobronchial Watanabe Spigot

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    Yuichiro Machida

    2015-01-01

    Full Text Available A bronchial fistula is one of the most serious complications that can occur following pulmonary lobectomy. We herein report a case of bronchial fistula that was successfully treated by endobronchial embolization using an Endobronchial Watanabe Spigot (EWS. A 72-year-old male underwent right lower lobectomy of the lung with nodal dissection for a pulmonary squamous cell carcinoma. A bronchial fistula developed 53 days after surgery. Tube drainage was performed, and air leakage was apparent. Under endoscopic observation, intrathoracic injection of indigo carmine revealed that a fistula existed at the peripheral site of the B2ai bronchus. After one EWS (small was inserted into the B2a bronchus tightly using a bronchoscope, the air leakage was stopped. Pleurodesis was further carried out, the thoracostomy tube was subsequently removed, and the patient was discharged. Endobronchial embolization using an EWS is an option for the treatment of a bronchial fistula after pulmonary resection.

  11. Medroxyprogesterone acetate attenuates long-term effects of 17beta-estradiol in coronary arteries from hyperlipidemic rabbits

    DEFF Research Database (Denmark)

    Pedersen, S. H.; Nielsen, L. B.; Mortensen, A

    2006-01-01

    in coronary arteries. METHODS: Watanabe heritable hyperlipidemic rabbits were treated orally with either E(2) (4 mg/day), medroxyprogesterone acetate (MPA) (10 mg/day), norethindrone acetate (NETA) (2 mg/day), E(2)+MPA, E(2)+NETA, or placebo for 16 weeks (n=10 in each group). Coronary arteries were used for m...

  12. Progestins oppose the effects of estradiol on the endothelin-1 receptor type B in coronary arteries from ovariectomized hyperlipidemic rabbits

    DEFF Research Database (Denmark)

    Pedersen, Susan H; Nielsen, Lars B; Mortensen, Alicja

    2008-01-01

    ) system in coronary arteries. DESIGN: Watanabe heritable hyperlipidemic rabbits were treated orally with either E2 (4 mg/d), medroxyprogesterone acetate (MPA) (10 mg/d), norethisterone acetate (NETA) (2 mg/d), E2 + MPA, E2 + NETA, or placebo for 16 weeks (n=10 in each group). Coronary arteries were used...

  13. Medroxyprogesterone acetate attenuates long-term effects of 17 beta-estradiol in coronary arteries from hyperlipidemic rabbits

    DEFF Research Database (Denmark)

    Pedersen, Susan H.; Nielsen, Lars Bo; Mortensen, Alicja

    2006-01-01

    arteries. Methods: Watanabe heritable hyperlipidemic rabbits were treated orally with either E-2 (4 mg/day), medroxyprogesterone acetate (MPA) (10 mg/day), norethindrone acetate (NETA) (2 mg/day), E-2 + MPA, E-2 + NETA, or placebo for 16 weeks (n = 10 in each group). Coronary arteries were used for m...

  14. Dietary boron does not affect tooth strength, micro-hardness, and density, but affects tooth mineral composition and alveolar bone mineral density in rabbits fed a high-energy diet.

    Science.gov (United States)

    Hakki, Sema S; SiddikMalkoc; Dundar, Niyazi; Kayis, Seyit Ali; Hakki, Erdogan E; Hamurcu, Mehmet; Baspinar, Nuri; Basoglu, Abdullah; Nielsen, Forrest H; Götz, Werner

    2015-01-01

    The objective of this study was to determine whether dietary boron (B) affects the strength, density and mineral composition of teeth and mineral density of alveolar bone in rabbits with apparent obesity induced by a high-energy diet. Sixty female, 8-month-old, New Zealand rabbits were randomly assigned for 7 months into five groups as follows: (1) control 1, fed alfalfa hay only (5.91 MJ/kg and 57.5 mg B/kg); (2) control 2, high energy diet (11.76 MJ and 3.88 mg B/kg); (3) B10, high energy diet + 10 mg B gavage/kg body weight/96 h; (4) B30, high energy diet + 30 mg B gavage/kg body weight/96 h; (5) B50, high energy diet + 50 mg B gavage/kg body weight/96 h. Maxillary incisor teeth of the rabbits were evaluated for compression strength, mineral composition, and micro-hardness. Enamel, dentin, cementum and pulp tissue were examined histologically. Mineral densities of the incisor teeth and surrounding alveolar bone were determined by using micro-CT. When compared to controls, the different boron treatments did not significantly affect compression strength, and micro-hardness of the teeth, although the B content of teeth increased in a dose-dependent manner. Compared to control 1, B50 teeth had decreased phosphorus (P) concentrations. Histological examination revealed that teeth structure (shape and thickness of the enamel, dentin, cementum and pulp) was similar in the B-treated and control rabbits. Micro CT evaluation revealed greater alveolar bone mineral density in B10 and B30 groups than in controls. Alveolar bone density of the B50 group was not different than the controls. Although the B treatments did not affect teeth structure, strength, mineral density and micro-hardness, increasing B intake altered the mineral composition of teeth, and, in moderate amounts, had beneficial effects on surrounding alveolar bone.

  15. DETECTING LOW DENSITY LIPOPROTEIN RECEPTOR MUTANT GENE OF RABBIT BY PCR

    Institute of Scientific and Technical Information of China (English)

    Liu Enqi; Zhao Sihai; Chen Zhenglan; Yang Penghui

    2006-01-01

    Objective Watanabe Heritable Hyperlipidaemic (WHHL) rabbits with low density lipoprotein receptor (LDLr) gene mutation have provided unprecedented opportunities for the study of human atherosclerosis, in order to confirm LDL receptor gene status in rabbits, we developed a simple PCR technique to detect LDL mutations in rabbits. Methods Rabbits genomic DNA were extracted from ear biopsy, and amplified by PCR to detect 12 bp deletion mutation in WHHL rabbits. PCR products were directly digested with BglⅠ, and then applied to polyacrylamide gel electrophoresis. Results PCR products from homozygous LDLr +/+ rabbits generated 2 bands of 212 and 94 bp after BglⅠ digestion, LDLr +/- rabbits generated 3 bands (294, 212, and 94 bp), LDLr -/- animals, however, generated only 1 product (294 bp). Conclusion This modified PCR method is simple and reliable.

  16. 多沙唑嗪对兔食饵性高脂血症作用%Effects of doxazosin on blood lipid levels in cholesterol-fed rabbits models

    Institute of Scientific and Technical Information of China (English)

    赵静惠; 杨梅; 闫胜敏; 段保湘

    2012-01-01

    目的 观察多沙唑嗪(DOX)对动脉粥样硬化兔模型血脂水平的影响.方法 健康雄性新西兰兔随机分为普通饮食组、高脂饮食组、DOX治疗组,每组10只.普通饮食组予普通饲料喂养,其余2组予高脂饲料.喂养4周后,DOX治疗组每日定时予DOX 1 ml/kg腹腔注射,连续给药9周.分别于实验开始时(0周、4周、7周、10周、13周)抽取空腹血标本,检测各组血清总胆固醇(TC)、三酰甘油(TG)和低密度脂蛋白胆固醇(LDL-C).结果 高脂饮食组兔血清TC第4周末(28.63±7.65)mmol/L,第13周末(40.83±3.90)mmol/L,血清LDL-C第4周末(16.21±9.59)mmol/L,第13周末(28.46±3.81)mmol/L,随高脂饲养的时间延长,血清TC、LDL-C水平进一步显著升高(P<0.05或0.05).结论 DOX对高血脂兔的血清TC、LDL-C 紊乱具有轻度的改善作用.%Objective To observe the effect of doxazosin ( DOX ) on blood lipid levels in rats with atherosclerosis. Methods New Zealand male rabbits were randomly divided into 3 groups:normal diet group,high fat diet group,DOX treatment group,with 10 rabbits in each group. The rabbits in normal diet group were fed with normal feed, however, the rabbits in the latter two groups were fed with high fat diet. After 4-week feeding, the rabbits in DOX treatment group were intraperitoneally injected with DOX lmg/kg,once a day for 9 weeks. The serum levels of TC,TG,LDL-C were detected at 0,4,7,10,13 weeks for the three groups. Results The levels of TC in high fat diet group were ( 28. 63 ±7. 65 )mmol/L at the end of 4 weeks,( 40. 83 ±3. 90 )mmol/L at the end of 13 weeks,and the levels of LDL-C were( 16.21 ±9.59 )mmol/L at the end of 4 weeks,( 28. 46 ±3. 81 )mmol/L at the end of 13 weeks respectively, with the time prolongation of high fat diet feeding,the levels of TC,LDL-C were increased further ( P 0. 05 ). Conclusion DOX can improve the disorder of LDL-C and TC mildly in the rabbits with hyperlipemia.

  17. Effects on growth performance, carcass characteristics, and the fat and meat fatty acid profile of rabbits fed diets with chia (Salvia hispanica L.) seed supplements.

    Science.gov (United States)

    Peiretti, P G; Meineri, G

    2008-12-01

    The effects of three levels (0%, 10%, or 15%) of chia (Salvia hispanica L.) seed (SHS) included in the diet on the growth performance, some carcass characteristics and fatty acid profile of rabbit meat and perirenal fat was studied. At the end of the experiment, there were no significant differences among the groups in live weight, live weight gain, feed consumption, feed efficiency, carcass yield or the percentages of edible organs. The percentage values of hind legs, fore legs, loin and abdominal wall, breast and ribs, skin and limbs, and head were not affected by the inclusion level of SHS. The polyunsaturated fatty acid (PUFA) concentration in the longissimus dorsi muscle and perirenal fat was significantly increased with increasing SHS inclusion, while the saturated fatty acid (SFA) decreased. The n-6/n-3 PUFA ratio of the rabbit meat decreased from 4.55 in the control group, to 1.03 in the 15% SHS group.

  18. Phospholipid Profile of the Stomach and Duodenum of Normal Rabbits Fed with Supplements of Unripe Pawpaw (Carica papaya) and Unripe Plantain (Musa sapientum) Extract

    Science.gov (United States)

    Eriyamremu, G. E.; Asagba, S. O.; Osagie, V. E.; Ojeaburu, S. I.; Lolodi, O.

    This study reports the effect of unripe pawpaw and/or unripe plantain extracts on the phospholipid profile of the stomach and duodenum of normal rabbits. Supplementation of chow with unripe pawpaw or plantain extract significantly reduced (p< 0.05) the weight gained by rabbits compared with the control. The total phospholipids content in the stomach and duodenum were significantly increased (p< 0.05) in the test groups compared to the control. In the stomach and duodenum, phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SGM) were significantly increased (p< 0.05) in the test groups compared to the control. Pawpaw occasioned a decrease in the PE/PC ratio in both tissues as against an increase observed in the SGM/PC ratio. The results presented suggests that unripe pawpaw meal and unripe plantain extract alter the phospholipid profile of the stomach and duodenum in ways which may affect membrane fluidity of these tissues and would have profound effect on the gastro-duodenal mucosa and thus have implication(s) for gastric and duodenal ulcers in rabbits.

  19. WEIGHTS or RABBITS

    African Journals Online (AJOL)

    heart and spleen from_male and femalerabbits were made, in rabbits fed graded levels (0, 4, 8, 12%) of oil palm indiets. ..... aeetyl CoA derived mainly from carbohydrate wasdepressed ... an integral part in the synthesis and metabolism of.

  20. Anti-hypercholesterolemic and anti-atherosclerotic effects of polarized-light therapy in rabbits fed a high-cholesterol diet.

    Science.gov (United States)

    Park, Dongsun; Kyung, Jangbeen; Kim, Dajeong; Hwang, Seock-Yeon; Choi, Ehn-Kyoung; Kim, Yun-Bae

    2012-03-01

    The effects of polarized-light therapy (PLT) on high-cholesterol diet (HCD)-induced hypercholesterolemia and atherosclerosis were investigated in comparison with that of lovastatin in rabbits. Hypercholesterolemia was induced by feeding male New Zealand white rabbits with 1% cholesterol in diet for 2 weeks and maintained with 0.5% cholesterol for 6 weeks, followed by normal diet for 2 weeks for recovery. Lovastatin (0.002% in diet) or daily 5-min or 20-min PLT on the outside surface of ears was started 2 weeks after induction of hypercholesterolemia. Hypercholesterolemic rabbits exhibited great increases in serum cholesterol and low-density lipoproteins (LDL) levels, and finally severe atheromatous plaques formation covering 57.5% of the arterial walls. Lovastatin markedly reduced both the cholesterol and LDL, but the reducing effect (47.5%) on atheroma formation was relatively low. By comparison, 5-min PLT preferentially decreased LDL, rather than cholesterol, and thereby potentially reduced the atheroma area to 42.2%. Notably, 20-min PLT was superior to lovastatin in reducing both the cholesterol and LDL levels as well as the atheromatous plaque formation (26.4%). In contrast to the increases in blood alanine transaminase and aspartate transaminase following lovastatin treatment, PLT did not cause hepatotoxicity. In addition, PLT decreased platelets and hematocrit level. The results indicate that PLT attenuates atherosclerosis not only by lowering blood cholesterol and LDL levels, but also by improving blood flow without adverse effects. Therefore, it is suggested that PLT could be a safe alternative therapy for the improvement of hypercholesterolemia and atherosclerosis.

  1. The Effect of EDTA and Garlic Extract Combination on Plasma Lipids, Lipoporteins, and Fatty Streaks in Cholesterol Fed Male Rabbit Aorta

    Directory of Open Access Journals (Sweden)

    M R Sharifi

    2004-12-01

    Full Text Available Background: Garlic extract is used in treatment of hypercholesterolemia, although its efficacy isn’t exactly clear. There is a little information about mechanism of garlic effect on plasma lipids. By intervention of garlic in atherogenesis process it has been shown that it has a protective effect against cardiovascular disease. On the other hand, it has been shown that EDTA (Ethylene Diamin Tetra Acetic Acid improves blood flow and decreases the vascular atherosclerotic symptoms. Methods: The objective of this study was to evaluate the effect of EDTA and garlic extract with together on plasma concentration of cholesterol, Triglyceride, LDL, and HDL, and formation of fatty streaks through the phenomena called “chelation therapy” in male Rabbit aorta. 32 male Rabbits were used and divided to 4 groups as group 1: cholesterol – rich diet (CRD + EDTA + Garlic Extract (GE, group 2: CRD + EDTA, Group 3: CRD + GE, Group 4: CRD (control group. The animals were under food and drug diet for 5 weeks. Blood samples of Rabbits were taken before and after the test and then plasma cholesterol, triglyceride, LDL and HDL were measured. Finally, abdominal aorta was studied and pathologically evaluated. Results: The experiments showed the significant decrease of mean cholesterol in case groups and mean triglyceride in the groups which have received EDTA + GE and EDTA. Our results showed that mean LDL and LDL/HDL Ratio in case groups have been decreased in comparison to control group. In addition, there was a significant increase in mean HDL in the group which has received EDTA + GE. Conclusion: In this study it was shown that the using of fresh garlic and EDTA with together has a reductive effect on cholesterol, Triglyceride, and LDL concentration and also has an increasing effect on HDL concentration. But it seems that concomitant using of these tow substances with together strengthen the effect of each one, and it produces a great reduction of the blood

  2. Overweight in young males reduce fertility in rabbit model

    National Research Council Canada - National Science Library

    Francisco Marco-Jiménez; José Salvador Vicente

    2017-01-01

    ... parameters and fertility success in randomized controlled trial in a rabbit model. Fourteen male rabbits were randomly assigned to a control group in which nutritional requirements were satisfied or a group fed...

  3. DETECTING LOW DENSITY LIPOPROTEIN RECEPTOR MUTANT GENE OF RABBIT BY PCR

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Many factors playi mportant roles inthe devel-opment of human atherosclerotic lesions,the lead-ing risk factor for atherosclerosis is familial hyper-cholesterolaemia(FH)[1-2].FHis a genetic diseasecharacterized by a deficiency of receptors for lowdensity lipoprotein(LDL)on the plas malemma ofendothelial cells,a high level of serum LDL,andearly development of atherosclerosis[3].WatanabeHeritable Hyperlipidaemic(WHHL)rabbits withunprovoked hypercholesterolaemia,increased bloodlevel of LDL,pronounced atheroscl...

  4. Respuesta productiva de conejos alimentados con forraje verde hidropónico de avena, como reemplazo parcial de concentrado comercial Productive response of rabbits fed with green hydroponic oats forage as partial replacement of commercial concentrate

    Directory of Open Access Journals (Sweden)

    Francisco Fabián Fuentes Carmona

    2011-04-01

    Full Text Available La respuesta productiva de conejos raza californiana alimentados con forraje verde hidropónico (FVH de avena como reemplazo parcial de concentrado comercial (CC, fue evaluada en condiciones de desierto en el norte de Chile. Se establecieron cinco tratamientos de alimentación, equivalentes a 0, 25, 50, 75 y 100% de reemplazo de la dieta diaria con FVH, el cual fue cosechado y utilizado directamente diez días después de la siembra. Cuarenta y cinco conejos destetados a los 31 días fueron evaluados en un diseño completamente al azar hasta alcanzar un peso de sacrificio de 2 kg de peso vivo. La calidad del FVH de avena fue considerada como buena, presentando similar valor nutritivo que el CC. Las variables evaluadas y sus respectivos rangos fueron: consumo de materia seca promedio (59.17 - 104.73 g/día, ganancia de peso vivo promedio (16.35 - 29.10 g/animal por día, conversión alimenticia promedio (3.31 - 3.93 kg MS/kg PV, tiempo de peso vivo a sacrificio (53 - 91 días, peso vivo final (1430 - 2044 g/animal, peso de la canal (1235 - 1385 g/animal y rendimiento de la canal (59.19 - 62.25%. El reemplazo de hasta 50% de la dieta base con FVH de avena no afectó significativamente (P The productive response of Californian rabbits fed with hydroponic green oats forage (HGOF as a partial replacement of commercial concentrate (CC was evaluated under desert conditions in northern Chile. Five treatments were established as follow: 0, 25, 50, 75 and 100% replacement of daily diet with HGOF, which was harvested and used directly at 10 days after sowing. Forty-five rabbits weaned at 31 days were evaluated in a completely randomized design until slaughter weight of 2.0 kg. HGOF quality was considered as good, presenting similar nutritional value to CC. The variables assessed and range values were: average dry matter intake (59.17-104.73 g/d, average weight gain (16.35-29.10 g/ rabbit for day, feed conversion average (3.31-3.93 kg DM/ kg LW, time of

  5. FXR-mediated down-regulation of CYP7A1 dominates LXRalpha in long-term cholesterol-fed NZW rabbits.

    Science.gov (United States)

    Xu, Guorong; Li, Hai; Pan, Lu-Xing; Shang, Quan; Honda, Akira; Ananthanarayanan, M; Erickson, Sandra K; Shneider, Benjamin L; Shefer, Sarah; Bollineni, Jaya; Forman, Barry M; Matsuzaki, Yasushi; Suchy, Frederick J; Tint, G Stephen; Salen, Gerald

    2003-10-01

    We investigated how cholesterol feeding regulates cholesterol 7alpha-hydroxylase (CYP7A1) via the nuclear receptors farnesoid X receptor (FXR) and liver X receptor alpha (LXRalpha) in New Zealand white rabbits. After 1 day of 2% cholesterol feeding, when the bile acid pool size had not expanded, mRNA levels of the FXR target genes short-heterodimer partner (SHP) and sterol 12alpha-hydroxylase (CYP8B) were unchanged, indicating that FXR activation remained constant. In contrast, the mRNA levels of the LXRalpha target genes ATP binding cassette transporter A1 (ABCA1) and cholesteryl ester transfer protein (CETP) increased 5-fold and 2.3-fold, respectively, associated with significant increases in hepatic concentrations of oxysterols. Activity and mRNA levels of CYP7A1 increased 2.4 times and 2.2 times, respectively. After 10 days of cholesterol feeding, the bile acid pool size increased nearly 2-fold. SHP mRNA levels increased 4.1-fold while CYP8B declined 64%. ABCA1 mRNA rose 8-fold and CETP mRNA remained elevated. Activity and mRNA of CYP7A1 decreased 60% and 90%, respectively. Feeding cholesterol for 1 day did not enlarge the ligand pool size or change FXR activation, while LXRalpha was activated highly secondary to increased hepatic oxysterols. As a result, CYP7A1 was up-regulated. After 10 days of cholesterol feeding, the bile acid (FXR ligand) pool size increased, which activated FXR and inhibited CYP7A1 despite continued activation of LXRalpha. Thus, in rabbits, when FXR and LXRalpha are activated simultaneously, the inhibitory effect of FXR overrides the stimulatory effect of LXRalpha to suppress CYP7A1 mRNA expression.

  6. Application of rabbits in biomedical research: a review.

    OpenAIRE

    Bosze, Zs.; Houdebine, L M

    2006-01-01

    The first transgenic rabbits were obtained two decades ago by pronuclear microinjection. Several characteristics of rabbit made it the first and classical model for the study of lipoproteins and atherosclerosis. Rabbit models include normal cholesterol-fed rabbits, spontaneous mutants for lipid metabolism and transgenic rabbits. Though most molecular investigations of the cardiovascular system have used transgenic mice, the small rodents do not accurately reflect crucial facets of human cardi...

  7. Desempenho reprodutivo de coelhas alimentadas com ração contendo diferentes fontes de óleo vegetal = Reproductive performance of rabbit does fed with rations containing different sources of vegetable oil

    Directory of Open Access Journals (Sweden)

    Márcia Aparecida Andreazzi

    2006-07-01

    Full Text Available Foram avaliadas 120 matrizes de coelhas durante dois ciclos reprodutivos. Os animais foram distribuídos em um delineamento experimental inteiramente casualizado, com 4 tratamentos (ração sem óleo e ração com 3% de óleo de canola, de óleo de milho ou de óleo de soja e 30 repetições. Foram coletados os dados de peso vivo das coelhas em cada cobrição, no parto, aos 21 e aos 35 dias de lactação e de consumo durante a gestação, os primeiros 21 dias de lactação e durante o período total de lactação (35 dias. Também foram obtidos os dados referentes ao peso vivo e ao número de láparos ao nascimento, aos 21 e aos 35 dias. Não foram observadas diferenças (P>0,05 entre os tratamentos quanto aos parâmetros de desempenho das coelhas no primeiro ciclo reprodutivo. No entanto os dados referentes ao segundo ciclo demonstraram maior peso vivo (P0,05 pela utilização das rações.One hundred and twenty rabbit does, were evaluated during two productive cycles. The animals were distributed in a completely randomized experimental design, with four treatments (oil-free rations and 3% canola oil, corn oil or soybean oil rations and 30 replications. While data of live weight of rabbit does were collected in each breeding, on birth, on the 21st and the 35th days of lactation, those on feed intake were recorded during gestation, onthe first 21 days of lactation and during the total period of lactation (35 days. Data on the litters live weight and number on birth, on the 21st and the 35th days, were also obtained. There were no differences (p>0.05 among treatments with regard to performance parameters of rabbit does on the first reproductive cycle. However, data concerning the second cycle demonstrated a better live weight (p0.05 by rations containing different sources of vegetable oil fed during the two reproductive cycles.

  8. Hypolipidemic and antioxidant activity of aqueous extract of fruit of Withania coagulans (Stocks) Dunal in cholesterol-fed hyperlipidemic rabbit model.

    Science.gov (United States)

    Shukla, Kirtikar; Dikshit, Piyush; Shukla, Rimi; Sharma, Sonal; Gambhir, Jasvinder K

    2014-09-01

    Withania coagulans (family: Solanaceae, English: Indian Cheese Maker, Hindi: Doda Paneer) fruit is known for its ethanopharmacological significance in health care system of India. Diet rich in high-fat is an important risk factor for diabetes, atherosclerosis and macro and microvascular complications. Treatment with aqueous extract of fruit of W. coagulans (aqWC; 250 mg/kg body weight) in cholesterol-fed animals resulted in significant decrease in the levels of total cholesterol, triacylglycerol, low density lipoprotein, tissue lipid content and acetyl CoA carboxylase activity whereas, the level of high density lipoprotein and activity of HMGCoA reductase also recovered partially. Treatment with aqWC also significantly decreased plasma lipid peroxide levels and increased reduced glutathione and superoxide dismutase activities. These results suggest that the aqueous extract of W. coagulans has potent lipid lowering and antioxidant activities.

  9. Desempenho produtivo e atividade microbiana cecal de coelhos alimentados com dietas contendo diferentes níveis de amido Performance and cecal microbial activity of growing rabbits fed diets with different starch levels

    Directory of Open Access Journals (Sweden)

    Alex Martins Varela de Arruda

    2000-06-01

    Full Text Available Com o objetivo de avaliar a influência do amido dietético sobre o rendimento produtivo e a atividade microbiana cecal de coelhos em crescimento, quatro dietas experimentais foram elaboradas de forma a conter níveis crescentes de amido (23, 28, 33 e 38% de amido total na base da MS, os quais foram fornecidos a 32 coelhos da raça Nova Zelândia Branco, distribuídos em gaiolas de engorda individuais, seguindo um delineamento em blocos casualizados. Houve efeito linear decrescente para consumo e conversão alimentar, em que valores de 108,67 g/d e 3,216, respectivamente, foram obtidos para os animais alimentados com 38% de amido dietético. No entanto, o ganho de peso e o rendimento de carcaça não foram influenciados pelos tratamentos. Por outro lado, os valores de pH, as concentrações totais e as proporções molares dos ácidos graxos voláteis não foram influenciados pelos tratamentos, porém, para o ácido propiônico, houve efeito linear decrescente, sendo a maior proporção molar (12,06% verificada no conteúdo cecal de coelhos alimentados com 23% de amido dietético. O milho-grão apresentou maior influência sobre a atividade microbiana cecal que o amido de milho purificado, porém, sem alterar o desempenho e rendimento de carcaça.With the objective to evaluate the effect of dietary starch on the productive yield and cecal microbial activity for rabbits in the growing, four experimental diets were formulated to contain increasing corn starch levels (23, 28, 33 and 38 % total starch levels in dry matter basis. A total of 32 White New Zealand rabbits were allotted to finishing cages in a completely randomized block design. The results showed a decreasing linear effect for the feed intake and feed:gain ratio, where values of 108.67g/d and 3.216, respectively, were recorded for animals fed 38% dietary starch. However, no difference was observed for the weight gain and carcass yield. Regarding to cecal microbial activity, pH and

  10. Influence of Hyperlipidemia on the Proliferation and Apoptosis of Myocardial Cells in Rabbits

    OpenAIRE

    Huse Kinscherf; Cynthia C. Haudenschild; Christian C. Chobanian

    2015-01-01

    Objective: To establish hyperlipemia models in rabbits fed with high fat diet, observe the changes of serum lipid level, and to explore the effect of hyperlipidemia on the proliferation and apoptosis of myocardial cells in rabbits. Methods: New Zealand white male special rabbits were randomly divided into 2 groups: normal diet group and high-fat-fed group, 10 cases in each group. The rabbits in normal group were fed with ordinary feed while the rabbits in the latter group were fed with high f...

  11. Effective Management of Persistent Pneumothorax Using a Thopaz® Digital Drainage System Combined with an Endobronchial Watanabe Spigot.

    Science.gov (United States)

    Shiroyama, Takayuki; Okamoto, Norio; Tamiya, Motohiro; Hamaguchi, Masanari; Tanaka, Ayako; Nishida, Takuji; Hayama, Manabu; Nishihara, Takashi; Morishita, Naoko; Suzuki, Hidekazu; Hirashima, Tomonori

    2016-01-01

    A 72-year-old man with salivary gland cancer and multiple pulmonary metastases suffering from intractable pneumothorax was transferred to our institution; he was inoperable because of a low pulmonary function. A chest tube had been placed more than a month prior to this admission. A digital drainage system was used for 24-h monitoring of air leaks (Thopaz®). Using the Thopaz® system, we performed endoscopic bronchial occlusion using an endobronchial Watanabe spigot (EWS) to reduce air leaks. Finally, the air leaks ceased, and the chest tube was removed five days after EWS placement. We herein report a case of persistent pneumothorax that was successfully treated by endoscopic bronchial EWS placement with the aid of a Thopaz® system.

  12. Endobronchial Watanabe spigot embolisation in the treatment of bronchopleural fistula due to tuberculous empyema in intensive care unit.

    Science.gov (United States)

    Dalar, Levent; Kosar, Filizs; Eryuksel, Emel; Karasulu, Levent; Altin, Sedat

    2013-01-01

    Tuberculosis may be complicated with empyema and fistula in patients with cellular immune deficiency. The case presented was a 39-year-old male patient with diagnosis of rheumatoid arthritis developed hydropneumothorax while taking steroid and immunosuppressive treatment and examination of pleural fluid revealed acid-fast bacilli. The patient was admitted to the intensive care unit due to respiratory failure and underwent bronchoscopic examination due to air leakage. The right middle lobe was obliterated by using an endobronchial Watanabe Spigot (EWS), and the amount of leakage decreased considerably after the procedure. On day 7, chest tube drainage was removed, and empyema was drained with a Pezzer drain. On day 50, upon the cessation of empyema drainage, spigots were removed with rigid and flexible bronchoscope. In conclusion, EWS use in the treatment of bronchopleural fistula is an effective, safe and a reversible procedure.

  13. problems and prospects of rabbit production in nigeria

    African Journals Online (AJOL)

    DR. AMINU

    with the rabbits being fed on crop residues, weeds, waste fruits, vegetables and poultry droppings. The manure can .... descendants of the European wild rabbit, Oryctolagus ... most popular in commercial rabbit industries in the .... and a water supply for cleaning purposes (Aduku and ..... Journal of Biotechnology, 2:6 34-39.

  14. Disposable rabbit

    Science.gov (United States)

    Lewis, Leroy C.; Trammell, David R.

    1986-01-01

    A disposable rabbit for transferring radioactive samples in a pneumatic transfer system comprises aerated plastic shaped in such a manner as to hold a radioactive sample and aerated such that dissolution of the rabbit in a solvent followed by evaporation of the solid yields solid waste material having a volume significantly smaller than the original volume of the rabbit.

  15. Disposal rabbit

    Science.gov (United States)

    Lewis, L.C.; Trammell, D.R.

    1983-10-12

    A disposable rabbit for transferring radioactive samples in a pneumatic transfer system comprises aerated plastic shaped in such a manner as to hold a radioactive sample and aerated such that dissolution of the rabbit in a solvent followed by evaporation of the solid yields solid waste material having a volume significantly smaller than the original volume of the rabbit.

  16. If Auditors Are Like Belgian Beers, Which Style Would You Prefer? : Discussion of “Auditor Style and Financial Statement Comparability” by Francis, Pinnuck, and Watanabe .

    NARCIS (Netherlands)

    Bruynseels, L.M.L.; Brenk, van H.M.

    2016-01-01

    Recent research by Francis, Pinnuck, and Watanabe (2014) has shown that financial reporting outcomes are influenced by the audit firm’s unique audit style. They argue that audit firm styles are driven by their “unique set of internal working rules that guide the auditor’s application of accounting a

  17. If Auditors Are Like Belgian Beers, Which Style Would You Prefer? : Discussion of “Auditor Style and Financial Statement Comparability” by Francis, Pinnuck, and Watanabe .

    NARCIS (Netherlands)

    Bruynseels, L.M.L.; Brenk, van H.M.

    2016-01-01

    Recent research by Francis, Pinnuck, and Watanabe (2014) has shown that financial reporting outcomes are influenced by the audit firm’s unique audit style. They argue that audit firm styles are driven by their “unique set of internal working rules that guide the auditor’s application of accounting

  18. Superovulação em coelhas alimentadas com ração, contendo diferentes fontes de óleos vegetais = Super-ovulation in rabbit does fed on rations containing different sources of vegetable oil

    Directory of Open Access Journals (Sweden)

    Márcia Aparecida Andreazzi

    2006-07-01

    wereobserved (p>0.05 in progesterone and 17β-estradiol serum levels, uterus plus ovaries weight, number of follicles and of corpora lutea in the right and left ovaries, number of viable and degenerated structures. Treatment affected (p<0.05 embryo production: it waslower in rabbit does fed on 3% canola oil rations and higher in those fed on vegetable oilfree rations.

  19. Roles of the WHHL Rabbit in Translational Research on Hypercholesterolemia and Cardiovascular Diseases

    Directory of Open Access Journals (Sweden)

    Tsutomu Kobayashi

    2011-01-01

    Full Text Available Conquering cardiovascular diseases is one of the most important problems in human health. To overcome cardiovascular diseases, animal models have played important roles. Although the prevalence of genetically modified animals, particularly mice and rats, has contributed greatly to biomedical research, not all human diseases can be investigated in this way. In the study of cardiovascular diseases, mice and rats are inappropriate because of marked differences in lipoprotein metabolism, pathophysiological findings of atherosclerosis, and cardiac function. On the other hand, since lipoprotein metabolism and atherosclerotic lesions in rabbits closely resemble those in humans, several useful animal models for these diseases have been developed in rabbits. One of the most famous of these is the Watanabe heritable hyperlipidemic (WHHL rabbit, which develops hypercholesterolemia and atherosclerosis spontaneously due to genetic and functional deficiencies of the low-density lipoprotein (LDL receptor. The WHHL rabbit has been improved to develop myocardial infarction, and the new strain was designated the myocardial infarction-prone WHHL (WHHLMI rabbit. This review summarizes the importance of selecting animal species for translational research in biomedical science, the development of WHHL and WHHLMI rabbits, their application to the development of hypocholesterolemic and/or antiatherosclerotic drugs, and future prospects regarding WHHL and WHHLMI rabbits.

  20. CURRENT STATUS, CHALLENGES AND OPPORTUNITIES OF RABBIT PRODUCTION IN BOTSWANA

    Directory of Open Access Journals (Sweden)

    J.C. MOREKI

    2012-03-01

    Full Text Available This review highlights the current status of rabbit production, challenges facing the industry and opportunities available. Rabbit farming in Botswana is in its infancy and the rabbit population is estimated to be less than 1000. However, this value is a gross underestimate due to poor monitoring by government extension services. In Botswana, rabbits are mainly kept in the backyards, indicating that intensive systems have not yet been developed. Rabbits have small body size, short gestation period, high reproductive potential, rapid growth rate and ability to utilize forages. Compared to beef, chicken, mutton, chevon and chicken, rabbit meat has low cholesterol, high protein and low fat contents. Rabbit production can be integrated into small farming systems, with the rabbits being fed on crop residues, weeds, poultry droppings, and kitchen and garden wastes. The manure can be used to fertilize soils. The major challenges in rabbit production are inadequacy of breeding stock, inadequate rabbit feeds, poor management (feeding, housing and health care, lack of research support, lack of technical support from extension services, lack of access to credit and inadequate supply of equipment. The major opportunity available to the rearers is that the market is vast due to the small rabbit population in the country. The attributes of rabbits suggest that rabbit farming is likely to play an important role in nutrition, poverty alleviation and food security, especially in countries with higher unemployment levels and HIV/AIDS prevalence rates such as Botswana.

  1. Bisphenol A exposure enhances atherosclerosis in WHHL rabbits.

    Directory of Open Access Journals (Sweden)

    Chao Fang

    Full Text Available Bisphenol A (BPA is an environmental endocrine disrupter. Excess exposure to BPA may increase susceptibility to many metabolic disorders, but it is unclear whether BPA exposure has any adverse effects on the development of atherosclerosis. To determine whether there are such effects, we investigated the response of Watanabe heritable hyperlipidemic (WHHL rabbits to 400-µg/kg BPA per day, administered orally by gavage, over the course of 12 weeks and compared aortic and coronary atherosclerosis in these rabbits to the vehicle group using histological and morphometric methods. In addition, serum BPA, cytokines levels and plasma lipids as well as pathologic changes in liver, adipose and heart were analyzed. Moreover, we treated human umbilical cord vein endothelial cells (HUVECs and rabbit aortic smooth muscle cells (SMCs with different doses of BPA to investigate the underlying molecular mechanisms involved in BPA action(s. BPA treatment did not change the plasma lipids and body weights of the WHHL rabbits; however, the gross atherosclerotic lesion area in the aortic arch was increased by 57% compared to the vehicle group. Histological and immunohistochemical analyses revealed marked increases in advanced lesions (37% accompanied by smooth muscle cells (60% but no significant changes in the numbers of macrophages. With regard to coronary atherosclerosis, incidents of coronary stenosis increased by 11% and smooth muscle cells increased by 73% compared to the vehicle group. Furthermore, BPA-treated WHHL rabbits showed increased adipose accumulation and hepatic and myocardial injuries accompanied by up-regulation of endoplasmic reticulum (ER stress and inflammatory and lipid metabolism markers in livers. Treatment with BPA also induced the expression of ER stress and inflammation related genes in cultured HUVECs. These results demonstrate for the first time that BPA exposure may increase susceptibility to atherosclerosis in WHHL rabbits.

  2. O processo aterosclerótico em artérias de coelhos submetidos a dieta suplementada com gema de ovo: modelo experimental de baixo custo Atherosclerotic lesion formation in rabbits fed on egg yolk-supplemented diet: an inexpensive experimental model

    Directory of Open Access Journals (Sweden)

    Rodrigo Gibin Jaldin

    2006-12-01

    Full Text Available OBJETIVO: Verificar a aterogenicidade do modelo de hipercolesterolemia por suplementação alimentar com gema de ovo em coelhos e seu uso como modelo de aterosclerose experimental de baixo custo. MATERIAL E MÉTODO: Foram utilizados 14 coelhos divididos em dois grupos de sete animais: grupo controle (G1, que recebeu ração comercial ad libitum, e grupo tratado (G2, que foi alimentado com dieta suplementada com gema de ovo. Ambos os grupos foram alimentados por 90 dias. Foram realizadas dosagens do perfil lipídico dos animais nos momentos 0, 30, 60 e 90 dias. Ao término do período experimental, os animais foram submetidos a eutanásia e retirada da aorta e de seus ramos diretos para realização de estudo anatomopatológico. RESULTADOS Apenas no grupo G2 houve aumento significativo nos níveis de colesterol total e frações. Ao exame macroscópico, foram observadas estrias gordurosas no arco aórtico e aorta abdominal e, à microscopia, acúmulos lipídicos discretos na íntima da aorta abdominal, renal, carótida, transição toracoabdominal e femoral. Portanto, a dieta com gema de ovo provocou aterosclerose leve no animal de experimentação e alterações equivalentes àquelas provocadas pelo colesterol purificado comercial quando fornecido em baixa dosagem. Assim sendo, a gema de ovo pode ser utilizada como fonte de colesterol alimentar de baixo custo em modelos de aterosclerose experimental.OBJECTIVE: To observe whether an inexpensive dietary-induced hypercholesterolemia model in rabbits using a chow diet supplemented with egg yolks can be used to develop atherosclerotic lesions in animals. MATERIAL AND METHOD: Fourteen rabbits were divided into two groups of seven animals: control group (G1, in which the rabbits were fed standard chow diet; and experimental group (G2, in which the rabbits were fed standard chow supplemented with egg yolks. Both groups were fed for 90 days. The lipid profile of all animals was measured at 0, 30, 60 and

  3. Assessment of Blood Chemistry, Weight Gain and Linear Body Measurements of Pre-Puberal Buck Rabbits Fed Different Levels of Neem (Azadirachta indica A. Juss. Leaf Meals Evaluación de Química Sanguínea, Ganancia de Peso y Mediciones Corporales Lineales de Conejos Pre-Púberes Alimentados con Diferentes Niveles de Harina de Hojas de Neem (Azadirachta indica A. Juss.

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    I.P Ogbuewu

    2010-09-01

    Full Text Available A 16 week feeding trial was carried out to investigate the effect of dietary Neem (Azadirachta indica A. Juss. leaf meal (NLM on body weight gain, linear body measurements and blood chemistry of pre-puberal buck rabbits. Four treatment diets were formulated to contain the NLM at inclusion levels of 0 (control, 5, 10 and 15%. Thirty six crossbred New Zealand white × Chinchilla pre-puberal buck rabbits aged 5 to 6 mo were divided into four groups of nine rabbits and each group was further replicated into three of three rabbits each. The rabbits were randomly assigned to the four dietary treatments. Lymphocyte count of rabbits fed control diet (8.32 × 10(9 mm-3 was significantly higher than the group fed 15% NLM (4.60 × 10(9 mm-3. The mean cell hemoglobin (MCH and mean cell volume (MCV of the control bucks were significantly (p 0.05 among the treatment groups. The results suggest that buck rabbits could tolerate up to 15% dietary inclusion of NLM without deleterious effects on body weight gain, linear body measurements and some hematological parameters.Se realizó un ensayo de alimentación de 16 semanas para investigar el efecto de harina de hojas de neem Azadirachta indica A. Juss. (NML sobre ganancia de peso, mediciones corporales lineales y química sanguínea de conejos machos pre-púberes. Se formularon cuatro dietas con niveles de inclusión de NLM de 0 (control, 5, 10, y 15%. Treinta y seis conejos híbridos New Zealand white × Chinchilla, pre-púberes, de 5 a 6 meses, se distribuyeron en cuatro grupos de nueve conejos, y cada grupo fue repetido en tres grupos de tres conejos cada uno. Los conejos se asignaron aleatoriamente a las cuatro dietas tratamiento. El recuento de linfocitos de los conejos alimentados con la dieta control (8,32 × 10(9 mm-3 fue significativamente mayor que el grupo alimentado con 15% NLM (4,60 × 10(9 mm-3. La hemoglobina celular media (MCH y el volumen celular medio (MCV de los conejos control fueron

  4. Strategies for rearing of rabbit does

    NARCIS (Netherlands)

    Rommers, J.M.

    2003-01-01

    This thesis describes the effects of different rearing strategies for young rabbit does on body development and reproduction performance. In current rearing, does are often fed to appetite from weaning to first insemination. First insemination is applied when 75 to 80% of mature body weight (BW) is

  5. Strategies for rearing of rabbit does

    NARCIS (Netherlands)

    Rommers, J.M.

    2003-01-01

    This thesis describes the effects of different rearing strategies for young rabbit does on body development and reproduction performance. In current rearing, does are often fed to appetite from weaning to first insemination. First insemination is applied when 75 to 80% of mature body weight (BW) is

  6. Atividade microbiana cecal e contribuição nutricional da cecotrofia em coelhos alimentados com rações contendo diferentes fontes de fibra e níveis de amido Caecal microbial activity and caecotrophy nutritional contribution in rabbits fed diets with different starch levels and fiber sources

    Directory of Open Access Journals (Sweden)

    Alex Martins Varela de Arruda

    2003-08-01

    Full Text Available Objetivou-se neste estudo a avaliação da atividade microbiana cecal e a contribuição nutricional da cecotrofia em coelhos alimentados com rações contendo diferentes níveis de amido (22 a 32% em média mediante a inclusão de milho grão e diferentes fontes de fibra (feno de alfafa ou casca de soja, em esquema fatorial 2x2. No primeiro experimento, 40 coelhos alojados em gaiolas de engorda individuais, em delineamento inteiramente casualizado e alimentados à vontade dos 45 aos 85 dias de idade, foram abatidos para coleta dos conteúdos ileais e cecais. A concentração de ácidos graxos voláteis foi influenciada pela interação entre tratamentos, sendo 93,82 mmol/l para ração de maior nível de amido com casca de soja e 80,03 mmol/l para ração de menor nível de amido com feno de alfafa. No segundo experimento, 40 coelhos aos 65 dias de idade, alojados em gaiolas de engorda individuais, em delineamento inteiramente casualizado, foram alimentados à vontade por 10 dias, e receberam colares de madeira para permitir a coleta total de cecotrofos. Houve influência da interação entre tratamentos sobre os teores de proteína e energia dos cecotrofos, sendo de 29,66% e 4204,87 kcal/kg para ração contendo maiores níveis de amido e casca de soja e de 27,98% e 4080,46 kcal/kg para ração contendo menores níveis de amido e feno de alfafa. As rações de alto amido ou com casca de soja propiciaram maior enriquecimento do conteúdo cecal e melhor aporte nutricional para os coelhos.The objective of this study was to evaluate the caecal microbial activity and caecotrophy nutritional contribution in rabbits fed diets with differents starch levels (22 or 32% on average from a high or low inclusion of corn grain and differents fiber sources (alfalfa hay or soybean hulls, in a 2x2 factorial design. In the first experiment, 40 rabbits were individually housed in fattening cages within entirely randomized design, and fed ad libitum from 45 to 85

  7. Rabbit hematology.

    Science.gov (United States)

    Marshall, Kemba L

    2008-09-01

    Using laboratory animal medicine as an established resource, companion animal veterinarians have access to many physiologic and basic science studies that we can now merge with our clinical impressions. By working with reference laboratories, companion animal veterinarians are poised to accelerate our knowledge of the normal rabbit rapidly. The aim of this article is to discuss normal hematopoiesis and infectious and metabolic diseases that specifically target the hemolymphatic system. Additionally, photographic representation of cell types is provided.

  8. NO-1886对高糖高脂饲料喂养新西兰兔糖代谢的影响%NO-1886 improves glucose metabolism in high fat/high sucrose-fed New Zealand white rabbits

    Institute of Scientific and Technical Information of China (English)

    张秋菊; 袁中华; 堤一彦; 谢谕湘; 王宗保; 付国香; 龙光; 尹卫东

    2003-01-01

    合成药NO-1886是脂蛋白脂肪酶(LPL)的激动剂,能降低血浆甘油三酯(TG)并升高高密度脂蛋白胆固醇(HDL-c)水平.我们曾发现NO-1886还具有降低血糖的作用.本研究主要观察NO-1886对糖尿病兔胰岛素抵抗及β-细胞功能方面的影响.用高糖高脂饲料诱导,使新西兰兔血浆葡萄糖升高,发生胰岛素抵抗.在高糖高脂饲料中添NO-1886进行治疗.结果发现NO-1886可抑制血清葡萄糖升高,经糖耐量和胰岛素敏感性试验检测,NO-1886可保护胰岛素的急性相分泌,增强胰岛素对葡萄糖的清除能力.研究结果提示NO-1886具有改善胰岛素抵抗、降低血糖的作用.%Synthetic compound NO-1886 is a lipoprotein lipase activator which lowers plasma triglycerides and elevates high-density lipoprotein cholesterol(HDL-C). Recently, we found that NO-1886 also had an action of reducing plasma glucose in high fat/high sucrose diet induced diabetic rabbit. In the current study, we investigated the effects of NO-1886 on insulin resistance and B-cell function in the rabbits. Our results showed that high fat/high sucrose feeding increased plasma glucose levels . This diet also induced insulin resistance, impairment of acute insulin response to glucose loading. Supplementing 1% NO-1886 into the high fat/high sucrose diet resulted in decreased serum glucose levels. We also found a clear increased glucose clearance and a protected acute insulin response to intravenous glucose load by NO-1886 supplementation. These data suggest that NO-1886 suppresses the elevation of blood glucose in rabbits induced by feeding a high fat/high sucrose diet probably through improving insulin resistance.

  9. Desempenho reprodutivo de coelhas alimentadas com ração contendo diferentes fontes de óleo vegetal - DOI: 10.4025/actascianimsci.v28i3.43 Reproductive performance of rabbit does fed with rations containing different sources of vegetable oil - DOI: 10.4025/actascianimsci.v28i3.43

    Directory of Open Access Journals (Sweden)

    Gentil Vanini de Moraes

    2006-01-01

    Full Text Available Foram avaliadas 120 matrizes de coelhas durante dois ciclos reprodutivos. Os animais foram distribuídos em um delineamento experimental inteiramente casualizado, com 4 tratamentos (ração sem óleo e ração com 3% de óleo de canola, de óleo de milho ou de óleo de soja e 30 repetições. Foram coletados os dados de peso vivo das coelhas em cada cobrição, no parto, aos 21 e aos 35 dias de lactação e de consumo durante a gestação, os primeiros 21 dias de lactação e durante o período total de lactação (35 dias. Também foram obtidos os dados referentes ao peso vivo e ao número de láparos ao nascimento, aos 21 e aos 35 dias. Não foram observadas diferenças (P>0,05 entre os tratamentos quanto aos parâmetros de desempenho das coelhas no primeiro ciclo reprodutivo. No entanto os dados referentes ao segundo ciclo demonstraram maior peso vivo (P0,05 pela utilização das raçõesOne hundred and twenty rabbit does, were evaluated during two productive cycles. The animals were distributed in a completely randomized experimental design, with four treatments (oil-free rations and 3% canola oil, corn oil or soybean oil rations and 30 replications. While data of live weight of rabbit does were collected in each breeding, on birth, on the 21st and the 35th days of lactation, those on feed intake were recorded during gestation, on the first 21 days of lactation and during the total period of lactation (35 days. Data on the litters live weight and number on birth, on the 21st and the 35th days, were also obtained. There were no differences (p>0.05 among treatments with regard to performance parameters of rabbit does on the first reproductive cycle. However, data concerning the second cycle demonstrated a better live weight (p0.05 by rations containing different sources of vegetable oil fed during the two reproductive cycles

  10. Variações em metabólitos no plasma e hormônios no soro sangüíneo de coelhas alimentadas com farelo de canola em substituição gradual ao farelo de soja Changes in metabolic plasma and serum hormones in female rabbits fed on diets with canola meal gradually replacing soybean meal

    Directory of Open Access Journals (Sweden)

    Lígia Vieira Lage

    2001-05-01

    Full Text Available O objetivo deste trabalho foi avaliar as possíveis variações dos níveis plasmáticos de glicose, triglicerídeos, colesterol total, uréia e os níveis do soro sanguíneo de estradiol, progesterona, insulina, T3 e T4 em coelhas alimentadas com dietas em que o farelo de canola substituiu em 00%, 33%, 66% e 100% o farelo de soja, no período de 43 aos 225 dias de idade (final da primeira lactação. Foram utilizadas quarenta coelhas da raça Nova Zelândia Branca, em um delineamento inteiramente casualizado, com 4 tratamentos e 10 repetições. As coelhas foram cobertas aos 150 dias de idade. Aos 43, 80, 150, 184 e 225 dias, foram realizadas as coletas de sangue por punção cardíaca. Os níveis plasmáticos de glicose, triglicerídeos, colesterol total e uréia não foram alterados pelos níveis de substituição do farelo de soja pelo farelo de canola. No entanto, houve efeito quadrático em relação à idade dos animais. Os níveis no soro sanguíneo de estradiol, progesterona, insulina, T3 e T4 não apresentaram efeito de tratamento. Os níveis de progesterona e T4 apresentaram efeito quadrático em relação à idade das coelhas. As variações encontradas em função da idade dos animais poderiam ser atribuídas às variações ambientais ou fisiológicas durante o período de realização do experimento.Possible changes in plasma levels of glucose, triglycerides, total cholesterol, urea, serum levels of estradiol, progesterone, insulin, T3 and T4 in female rabbits are evaluated. Rabbits were fed on soybean meal in a gradual substitution (00%, 33%, 66% and 100% by canola meal. Rabbits aged 43 to 225 days, on completing first pregnancy and lactation. Forty White New Zealand rabbits female were used in a completely randomized design, with four treatments and 10 replications. At 43, 80, 150, 184 and 225 days old the animals underwent heart puncture for blood collection. Females were mated at 150 days. Levels of glucose plasma

  11. Viral infections of rabbits.

    Science.gov (United States)

    Kerr, Peter J; Donnelly, Thomas M

    2013-05-01

    Viral diseases of rabbits have been used historically to study oncogenesis (e.g. rabbit fibroma virus, cottontail rabbit papillomavirus) and biologically to control feral rabbit populations (e.g. myxoma virus). However, clinicians seeing pet rabbits in North America infrequently encounter viral diseases although myxomatosis may be seen occasionally. The situation is different in Europe and Australia, where myxomatosis and rabbit hemorrhagic disease are endemic. Advances in epidemiology and virology have led to detection of other lapine viruses that are now recognized as agents of emerging infectious diseases. Rabbit caliciviruses, related to rabbit hemorrhagic disease, are generally avirulent, but lethal variants are being identified in Europe and North America. Enteric viruses including lapine rotavirus, rabbit enteric coronavirus and rabbit astrovirus are being acknowledged as contributors to the multifactorial enteritis complex of juvenile rabbits. Three avirulent leporid herpesviruses are found in domestic rabbits. A fourth highly pathogenic virus designated leporid herpesvirus 4 has been described in Canada and Alaska. This review considers viruses affecting rabbits by their clinical significance. Viruses of major and minor clinical significance are described, and viruses of laboratory significance are mentioned. Copyright © 2013 Elsevier Inc. All rights reserved.

  12. Dietary Niacin Supplementation Suppressed Hepatic Lipid Accumulation in Rabbits.

    Science.gov (United States)

    Liu, Lei; Li, Chunyan; Fu, Chunyan; Li, Fuchang

    2016-12-01

    An experiment was conducted to investigate the effect of niacin supplementation on hepatic lipid metabolism in rabbits. Rex Rabbits (90 d, n = 32) were allocated to two equal treatment groups: Fed basal diet (control) or fed basal diet with additional 200 mg/kg niacin supplementation (niacin). The results show that niacin significantly increased the levels of plasma adiponectin, hepatic apoprotein B and hepatic leptin receptors mRNA (p0.05). However, niacin treatment significantly inhibited the hepatocytes lipid accumulation compared with the control group (prabbits.

  13. Comparison of the Effects of Coconut Oil and Soyabean Oil on TSH Level and Weight Gain in Rabbits

    Directory of Open Access Journals (Sweden)

    Vidushi Gupta

    2009-05-01

    Full Text Available The present study was conducted on 12 albino rabbits of either sex and weighing between 1-1.5kg to see the influence of coconut oil and soyabean oil on serum TSH levels and weight gain for a period of 12 weeks.  The rabbits were divided into 2 groups of six each.  Rabbits in group 1 were fed on coconut oil and in group 2 were fed on soyabean oil in addition to their standard diet.  At the end of 12 weeks we found that rabbits fed on soyabean oil had significant increase in TSH levels (p= 0.003 and gained more weight (p=0.000 when compared to rabbits fed on coconut oil.

  14. Becoming-Animal in Asian Americas: Ruthanne Lum McCunn’s God of Luck and a Watanabean Triptych (Three Poems by José Watanabe

    Directory of Open Access Journals (Sweden)

    Michelle Har Kim

    2012-06-01

    Full Text Available Considering the implicit North American and Anglophone core of Asian American literature traditionally conceived, this essay discusses two examples of literatures of the Asian Americas. A narrative of a Chinese coolie’s heroic escape from a Peruvian guano mine, Ruthanne Lum McCunn’s novel God of Luck (2008 introduces a lesser-known point of view to the field: the nineteenth-century Chinese coolie in Peru. Rather than embrace the emblematic hero who accedes to voice, this essay attempts to read outside of an anticipated rubric of individual politico-economic repletion. In the poetry of Peruvian writer José Watanabe (1946–2007, motifs of animal encounter abound—yet dogs, fish, and other kinds of life are never deployed as a discrete metaphor through which we can see and know ourselves. As readers we are shifted to the edge of the world, in a “becoming-animal” that explores not the Asian American, but its restless morphing, illegibly human or otherwise.

  15. Inlfuence of Hyperlipidemia on the Proliferation and Apoptosis of Myocardial Cells in Rabbits

    Institute of Scientific and Technical Information of China (English)

    Huse Kinscherf; Cynthia C Haudenschild; Christian C Chobanian

    2015-01-01

    Objective:To establish hyperlipemia models in rabbits fed with high fat diet, observe the changes of serum lipid level, and to explore the effect of hyperlipidemia on the proliferation and apoptosis of myocardial cells in rabbits. Methods:New Zealand white male special rabbits were randomly divided into 2 groups:normal diet group and high-fat-fed group, 10 cases in each group. The rabbits in normal group were fed with ordinary feed while the rabbits in the latter group were fed with high fat diet. The levels of serum TC, TG, HDL-C and LDL-C were detected at 0, 4th, 7th, 10th, 13th weeks for 2 groups. At the end of the 13th week, the pathological changes of myocardial tissues were detected by HE staining, and the proliferation and apoptosis of cardiomyocyte were tested by FCM. Results:After given high fat diet, the level of serum TC, TG, HDL-C and LDL-C in high-fat-fed diet group were signiifcantly increased than in the normal diet group from the 4th week to the 13th week (P0.05). Conclusion:Hyperlipidemia can induce the pathological changes of cardiomyocyte in rabbits, inhibit the proliferation of cardiomyocyte, but has no effect on the apoptosis of cardiomycyte in rabbits.

  16. Utilización de piensos criollos con harina de Albizia lebbeck para la ceba de conejos alimentados con bejuco de boniato Use of home-made concentrate feed with Albizia lebbeck meal for fattening rabbits fed sweet potato vines

    Directory of Open Access Journals (Sweden)

    I. L Montejo

    2010-03-01

    Full Text Available Se realizó un experimento durante 40 días en el módulo de animales menores de la Estación Experimental de Pastos y Forrajes "Indio Hatuey", con el objetivo de evaluar la inclusión de la harina de Albizia lebbeck en un pienso criollo con diferentes suplementos energético-proteicos, para la alimentación de conejos de ceba. Se utilizaron 26 animales mestizos sanos, de 60 días de edad y 0,94 (± 0,10 kg de peso, en un diseño completamente aleatorizado con dos tratamientos (13 animales por cada uno; estos fueron: A 100% bejuco de boniato (Ipomoea batata y B 70% bejuco de boniato más 30% de pienso criollo elaborado con harina de A. lebbeck y diferentes suplementos energético-proteicos. El alimento se ofreció en los horarios de la mañana (9:00-10:00 a.m. y la tarde (3:00-4:00 p.m.. Se encontraron diferencias significativas en el peso final de los animales (A-1,47 kg y B-1,62 kg. Las mejores ganancias de peso vivo se observaron en el tratamiento suplementado B (16,3 g/animal/día, que fueron superiores (PA trial was conducted for 40 days in the small animal facility of the Experimental Station of Pastures and Forages "Indio Hatuey", with the objective of evaluating the inclusion of Albizia lebbeck meal in a home-made concentrate feed with different energy-protein supplements, to feed fattening rabbits. Twenty-six healthy crossbred animals, 60 days old and with 0,94 (±0,10 kg of weight, were used in a completely randomized design with two treatments (13 animals each; they were: A 100% sweet potato (Ipomoea batata vines and B 70% sweet potato vines + 30% home-made concentrate feed elaborated with A. lebbeck meal and different energy-protein supplements. The feed was supplied in the morning (9:00-10:00 a.m. and the afternoon (3:00-4:00 p.m.. Significant differences were found in the final weight of the animals (A-1,47 kg and B-1,62 kg. The best live weight gains were observed in the supplemented treatment B (16,3 g/animal/day, which were

  17. Parâmetros biológicos de fêmeas adultas Amblyomma cajennense alimentadas em coelhos tratados com bioterápico ultradiluído Biological parameters of Amblyomma cajennense adult females fed on rabbits treated with ultradiluted biotherapic

    Directory of Open Access Journals (Sweden)

    Luciana Rodrigues de Almeida

    2008-08-01

    made with A. cajennense male and female adult ticks. Significant alterations were not observed on biological parameters of females A. cajennense fed on rabbits treated with the biotherapic, when compared to the control group. New tests, involving other host species and the attendance of subsequent infestations should be developed to evaluate the therapeutic / profilatic potential of the ultra-diluted biotherapics on infestation of the domestic animals.

  18. The Rabbit Stream Cipher

    DEFF Research Database (Denmark)

    Boesgaard, Martin; Vesterager, Mette; Zenner, Erik

    2008-01-01

    The stream cipher Rabbit was first presented at FSE 2003, and no attacks against it have been published until now. With a measured encryption/decryption speed of 3.7 clock cycles per byte on a Pentium III processor, Rabbit does also provide very high performance. This paper gives a concise...... description of the Rabbit design and some of the cryptanalytic results available....

  19. Hypercholesterolemia Impaired Sperm Functionality in Rabbits

    Science.gov (United States)

    Monclus, Maria A.; Cabrillana, Maria E.; Clementi, Marisa A.; Espínola, Leandro S.; Cid Barría, Jose L.; Vincenti, Amanda E.; Santi, Analia G.; Fornés, Miguel W.

    2010-01-01

    Hypercholesterolemia represents a high risk factor for frequent diseases and it has also been associated with poor semen quality that may lead to male infertility. The aim of this study was to analyze semen and sperm function in diet-induced hypercholesterolemic rabbits. Twelve adult White New Zealand male rabbits were fed ad libitum a control diet or a diet supplemented with 0.05% cholesterol. Rabbits under cholesterol-enriched diet significantly increased total cholesterol level in the serum. Semen examination revealed a significant reduction in semen volume and sperm motility in hypercholesterolemic rabbits (HCR). Sperm cell morphology was seriously affected, displaying primarily a “folded head”-head fold along the major axe-, and the presence of cytoplasmic droplet on sperm flagellum. Cholesterol was particularly increased in acrosomal region when detected by filipin probe. The rise in cholesterol concentration in sperm cells was determined quantitatively by Gas chromatographic-mass spectrometric analyses. We also found a reduction of protein tyrosine phosphorylation in sperm incubated under capacitating conditions from HCR. Interestingly, the addition of Protein Kinase A pathway activators -dibutyryl-cyclic AMP and iso-butylmethylxanthine- to the medium restored sperm capacitation. Finally, it was also reported a significant decrease in the percentage of reacted sperm in the presence of progesterone. In conclusion, our data showed that diet-induced hypercholesterolemia adversely affects semen quality and sperm motility, capacitation and acrosomal reaction in rabbits; probably due to an increase in cellular cholesterol content that alters membrane related events. PMID:20976152

  20. Hypercholesterolemia impaired sperm functionality in rabbits.

    Directory of Open Access Journals (Sweden)

    Tania E Saez Lancellotti

    Full Text Available Hypercholesterolemia represents a high risk factor for frequent diseases and it has also been associated with poor semen quality that may lead to male infertility. The aim of this study was to analyze semen and sperm function in diet-induced hypercholesterolemic rabbits. Twelve adult White New Zealand male rabbits were fed ad libitum a control diet or a diet supplemented with 0.05% cholesterol. Rabbits under cholesterol-enriched diet significantly increased total cholesterol level in the serum. Semen examination revealed a significant reduction in semen volume and sperm motility in hypercholesterolemic rabbits (HCR. Sperm cell morphology was seriously affected, displaying primarily a "folded head"-head fold along the major axe-, and the presence of cytoplasmic droplet on sperm flagellum. Cholesterol was particularly increased in acrosomal region when detected by filipin probe. The rise in cholesterol concentration in sperm cells was determined quantitatively by Gas chromatographic-mass spectrometric analyses. We also found a reduction of protein tyrosine phosphorylation in sperm incubated under capacitating conditions from HCR. Interestingly, the addition of Protein Kinase A pathway activators -dibutyryl-cyclic AMP and iso-butylmethylxanthine- to the medium restored sperm capacitation. Finally, it was also reported a significant decrease in the percentage of reacted sperm in the presence of progesterone. In conclusion, our data showed that diet-induced hypercholesterolemia adversely affects semen quality and sperm motility, capacitation and acrosomal reaction in rabbits; probably due to an increase in cellular cholesterol content that alters membrane related events.

  1. Avaliação do desempenho de coelhas alimentadas com farelo de canola em substituição parcial ou total ao farelo de soja Evaluation of the performance of female rabbits fed on canola meal in partial and total substitution of crude protein by soybean meal

    Directory of Open Access Journals (Sweden)

    Ivanor Nunes do Prado

    2001-05-01

    Full Text Available The effect of partial and total substitution of soybean meal crude protein (SBM by canola meal (CM on live weight (LW, daily weight gain (DWG, daily feed intake (DFI and feed conversion (FC in female rabbits are provided. Rabbits were evaluated from weaning period (43 days of age to adult life when they are capable of reproduction (150 days of age. Forty-eight White New Zealand females were used and distributed in four treatments. SBM was substituted at 00%, 33%, 66% and 100% levels by CM. The partial or total substitution of SBM by CM did not change (P > 0.05 the LW after 70 days old. Dunnett’s test showed that, independently of substitution level, FC of animals fed on meal with 33% SBM substitution by CM from age 43 to 90 days and 43 to 150 days was worse than that of animals fed on reference meal. LW120, DWG of 43 – 120-day-old animals receiving 33% SBM substitution by CM and LW150 and DWG of 43 – 150-day-old animals receiving meal with SBM substitution by 33% and 66% of CM were lower than those for animals that received reference meal. Excluding the reference meal, SBM substitution by CM during the periods in which animals were 43 - 120 days old and 43 - 150 days old caused an increase of DFI. Independently of level of inclusion as a substitute for SBM, results show that CM worsened the animals’ performance.Objetivando-se avaliar os efeitos da substiuição parcial e total do farelo de soja pelo farelo de canola sobre o peso, ganho médio diário (GMD, ingestão de alimentos (IA e conversão alimentar (CA em coelhas do desmame (43 dias até a idade de reprodução (150 dias, quarente e oito coelhas da raça Nova Zelândia, foram distribuídas, inteiramente ao acaso, em um experimento com quatro tratamentos e doze repetições. O farelo de soja foi substituído em 00, 33, 66 e 100% pelo farelo de canola. A substituição parcial ou total do farelo de soja não alterarou o peso após os 70 dias de idade. Por meio do uso do teste de

  2. Performance, digestibility and morphometry of ileal villi of rabbits fed with levels of inclusion of “Lithothamnium” Desempenho, digestibilidade e morfometria da vilosidade ileal de coelhos alimentados com diferentes níveis de inclusão de “Lithothamnium”

    Directory of Open Access Journals (Sweden)

    Roberto Maurício Carvalho Guedes

    2010-03-01

    Full Text Available In order to evaluate the potential of seaweed meal (Lithothamnium sp in the nutrition of rabbits it were realized analysis of the presence of trace elements in the composition of Lithothamnium, a performance test with determination of apparent digestibility coefficients for dry matter, protein and energy and morphometric analysis of the ileal villi of rabbits fed with diets at different levels of inclusion (0.25%, 0.50%, 0.75% and 1.00%. We used 60 New Zealand White rabbits of both sexes, at 30 days of age.The experimental diets were isonitrogenous and isocaloric, a control and the other with the different levels of inclusion of Lithothamnium sp. to replace the inert (bentonite from the control diet ad libitum. The experimental design was completely randomized, with five treatments and twelve repetitions. The variables were subjected to analysis of variance and regression analysis model with pre-defined by the statistical program SAEG. The results showed that the inclusion of Lithothamnium sp. in different concentrations did not interfere in the variables studied: intake, digestibility coefficients of dry matter, protein and energy, weight gain, carcass yield and weight of edible offal. However, it was verified that the level of inclusion of 1.0% interfere in a negative way in the length and width of villi.Com o intuito de avaliar o potencial da farinha de algas marinhas (Lithothamnium sp na nutrição de coelhos, realizaram-se análises de presença de elementos traço na composição do Lithothamnium, um ensaio de desempenho com determinação dos coeficientes de digestibilidade aparente para matéria seca, proteína e energia e análise morfométrica da vilosidade ileal de coelhos alimentados com dietas de diferentes níveis de inclusão (0,25%; 0,50%; 0,75% e 1,00%. Foram utilizados 60 coelhos Nova Zelândia Branco, de ambos os sexos, com 30 dias de idade. As dietas experimentais foram isoproteicas e isoenergéticas, uma controle e as demais

  3. Digestibilidade e atividade enzimática intestinal de coelhos em crescimento alimentados com diferentes fontes de amido processadas ou não por extrusão Digestibility and intestinal enzymatic activity of growing rabbits fed different sources of starch processed or not by extrusion

    Directory of Open Access Journals (Sweden)

    Luciana Kazue Otutumi

    2005-04-01

    Full Text Available Foram conduzidos dois experimentos para avaliar a digestibilidade do amido e a atividade específica das enzimas amilase (ATAM e maltase (ATMAL, em duas porções do intestino delgado (jejuno e íleo, em coelhos com idades iniciais de 28 e 49 dias, alimentados com quatro fontes de amido (milho, sorgo, triticale e mandioca, processados ou não por extrusão. Em cada experimento, foram utilizados 64 coelhos da raça Nova Zelândia Branco, distribuídos em um delineamento inteiramente casualizado com oito tratamentos e oito repetições. Ao final do período experimental, os animais foram abatidos para retirada de amostra do conteúdo do jejuno e íleo, além da raspagem da mucosa do jejuno, para determinação da ATAM e ATMAL. O processamento melhorou o coeficiente de digestibilidade (CD do amido do milho e do sorgo. Entre as fontes não-extrusadas, os maiores CD do amido foram apresentados pelo triticale e pela mandioca. A ATAM da parede do jejuno foi maior aos 60 dias. A ATMAL no conteúdo do jejuno e do íleo foi maior aos 37 dias. As diferentes fontes de amido diferiram entre si quanto à ATMAL e ATAM do conteúdo do jejuno e do íleo. A ATMAL foi maior no jejuno. Maiores ATAM do conteúdo do jejuno ocorreu aos 60 dias para os animais que receberam milho extrusado e sorgo. A ATAM aos 37 dias foi maior no íleo em comparação com o jejuno para os animais que receberam milho e sorgo. O processamento foi efetivo para o milho e sorgo, porém, todas as fontes, processadas ou não, apresentaram bom CD do amido, podendo ser utilizadas na alimentação de coelhos.Two experiments were carried out to study the digestibility of starch and the specific activity of amylase (ACAMY and maltase (ACMAL enzymes in two portions of small intestine (jejunum and ileum, in rabbits with initial ages of 28 and 49 days, fed with four sources of starch (corn, sorghum, triticale and cassava processed or not by extrusion. Sixty-four White New Zealand rabbits were used in

  4. Histological analysis of ovaries of female rabbits fed on canola meal gradually substituting soybean meal Análises histológicas do ovário de coelhas alimentadas com farelo de canola em substituição gradativa ao farelo de soja

    Directory of Open Access Journals (Sweden)

    Lígia Vieira Lage

    2001-05-01

    Full Text Available A histological study of New Zealand White female rabbits’ ovaries was undertaken. Rabbits were fed on canola meal as an increasing substitute (00, 33, 66 and 100% for soybean meal so that the tissue’s general aspects could be verified and the number of ovarian follicles from each experimental group could be obtained. Forty-eight female rabbits were slaughtered at 43, 150 and 225 days of age and their ovaries were fixed in Bouin’s liquid, washed and conserved in 70% ethanol and embedded in paraffin. 5µm-thick histological sections were undertaken and stained by HE and Azan techniques. The ovaries were bound by a predominantly simple cubic epithelium followed by the tunica albuginea. Ovarian follicles were observed in different stages of development in the cortical tissue. Interstitial gland cells, blood and lymphatic vessels and nerves were observed in the medullar tissue. Small cysts were also reported. Histological preparations didn’t show any change between groups either in the general morphology of the tissue or in follicle number. This fact suggests that the use of canola as a food of rabbits didn’t interfere in reproduction.O objetivo deste trabalho foi estudar a histologia de ovário de coelhas da raça Nova Zelândia Branca. As coelhas foram alimentadas com farelo de canola em substituição gradativa (00, 33, 66 e 100% ao farelo de soja. Foram estudados os aspectos histológicos gerais dos ovários, assim como a contagem de folículos. Um grupo de 48 coelhas foi dividido ao acaso e abatido aos 43, 150 e 225 dias de idade, e os ovários foram fixados em Bouin, lavados e conservados em solução de etanol a 70% e embebidos em parafina. Cortes histológicos foram realizados com 5µm de espessura e corados com HE e técnica de Azan. Os ovários apresentavam predominantemente epitélio simples cúbico seguidos de túnica albugínea. Os folículos foram observados em diferentes estágios de desenvolvimento no estroma cortical. C

  5. Variant rabbit hemorrhagic disease virus in young rabbits, Spain.

    Science.gov (United States)

    Dalton, Kevin P; Nicieza, Inés; Balseiro, Ana; Muguerza, María A; Rosell, Joan M; Casais, Rosa; Álvarez, Ángel L; Parra, Francisco

    2012-12-01

    Outbreaks of rabbit hemorrhagic disease have occurred recently in young rabbits on farms on the Iberian Peninsula where rabbits were previously vaccinated. Investigation identified a rabbit hemorrhagic disease virus variant genetically related to apathogenic rabbit caliciviruses. Improved antivirus strategies are needed to slow the spread of this pathogen.

  6. THE EFFECT OF CORTISONE ON THE SERUM LIPIDS AND ON THE DEVELOPMENT OF EXPERIMENTAL CHOLESTEROL ATHEROSCLEROSIS IN THE RABBIT

    Science.gov (United States)

    Gordon, Dina; Kobernick, Sidney D.; McMillan, Gardner C.; Duff, G. Lyman

    1954-01-01

    An experiment was performed to determine the effect of cortisone on the serum lipids and on the development of experimental cholesterol atherosclerosis in the rabbit. Litter mate rabbits of the same sex were employed; both sexes were represented in the experiment. The report is based upon four experimental groups comprising (1) 12 rabbits fed cholesterol and treated with cortisone vehicle; (2) 12 rabbits fed cholesterol and treated daily with cortisone; (3) 11 rabbits treated with cortisone; and (4) 7 rabbits that received cortisone vehicle. It was observed that: (1) There was less aortic atherosclerosis in the cholesterol-fed cortisone-treated rabbits as judged by both morphological and chemical means than in the rabbits fed cholesterol without cortisone treatment. (2) Cortisone depressed appreciably the hypercholesterolemia resulting from the feeding of cholesterol to rabbits. (3) Cortisone treatment caused a moderate hypercholesterolemia in normal rabbits. (4) Cortisone caused a moderate increase in serum lipid phosphorus equal to that produced by cholesterol feeding alone. (5) The combination of cholesterol feeding and cortisone did not result in a higher phospholipidemia than either one of these agents alone. (6) Cortisone caused a great increase in serum-neutral fat; it was not apparent whether cholesterol feeding affected the neutral fat lipemia due to cortisone treatment alone. (7) The total cholesterol to lipid phosphorus ratio was about normal in the rabbits that received cortisone only. It was doubled in the animals receiving both cholesterol and cortisone, and it was increased about four times in those that received only cholesterol. The significance of the alterations in the serum lipids induced by cortisone is discussed in relation to the inhibition of the development of aortic atherosclerosis that occurred in the cholesterol-fed rabbits treated with cortisone. PMID:13152282

  7. Haematological and serum biochemical response of growing rabbit ...

    African Journals Online (AJOL)

    STORAGESEVER

    2008-12-03

    Dec 3, 2008 ... Serum total protein of rabbits fed diets 3 (5.20 g/dl) and 4. (5.03 g/dl) were identical but ... components have measurable effects on blood components; hence, blood constituents ..... concentrate supplements. BOWEN J. Agric.

  8. Liver and kidney lesions and associated enzyme changes induced in rabbits by chronic cyanide exposure.

    Science.gov (United States)

    Okolie, N P; Osagie, A U

    1999-07-01

    The effect of prolonged chronic cyanide exposure on liver and kidney integrity, as well as some associated enzyme and metabolite changes, were investigated in New Zealand white rabbits (initial mean weight 1.52 kg) using a combination of colorimetric, spectrophotometric, enzymatic, gravimetric and histological procedures. Two groups of rabbits were fed for 40 weeks on either pure growers' mash or growers' mash containing 702 ppm inorganic cyanide. Results obtained indicate that the cyanide-fed rabbits had significantly decreased liver activities of alkaline phosphatase, glutamate pyruvate transaminase and sorbitol dehydrogenase relative to controls (Pactivities of these enzymes in the cyanide-treated group. Kidney alkaline phosphatase activity was significantly decreased (Pactivities of lactate dehydrogenase. In addition, liver and kidney rhodanese activities were significantly raised in the cyanide-fed group. There were marked degenerative changes in the liver and kidney sections from the cyanide-treated rabbits. These results suggest that chronic cyanide exposure may be deleterious to liver and kidney functions.

  9. Effects of Khaya senegalensis leaves on performance, carcass traits, hemtological and biochemical parameters in rabbits

    Science.gov (United States)

    Abdel-Wareth, A. A. A.; Hammad, Seddik; Ahmed, Hassan

    2014-01-01

    One of the challenges facing farmers today is to ensure adequate integration of natural resources into animal feeds. The aim of the present study is to evaluate the effects of Khaya senegalensis (KS) leaves on the performance of growing male rabbits, carcass traits and biochemical as well as hematological parameters. Thirty New Zealand White male growing rabbits were randomly divided into 3 groups (10 rabbits per group). Group I (control) received standard rabbit diet. Rabbits in group II and group III were fed standard rabbit diet supplemented with 35 % and 65 % KS leaves, respectively. All rabbits were fed daily for 25 days. The performance parameters and carcass criteria, including daily body weight gain, final body weight, and the percentage of dressing, were increased in rabbits fed 35 % KS when compared to the control group. Kidney and liver weight ratios increased significantly in group II but dropped in group III. Furthermore, liver enzymes - alanine aminotransferase and aspartate transaminase and kidney function parameters - urea, and creatinine - increased in both group II (significant Prabbit's diet with KS leaves. However, KS leaves may adversely affect liver and kidney function in a dose-dependent manner. Therefore, further studies are required to elucidate the maximum tolerable and toxic, as well as lethal doses, and to isolate the pharmacologically active components from KS leaves. PMID:26417277

  10. Effect of Nicardipine on Fasting Plasma Lipids and Apolipoproteins in Male New Zealand White Rabbits

    OpenAIRE

    Kamsiah, Jaarin; Nafeeza, MI

    1999-01-01

    The effect of nicardipine on fasting plasma lipid profiles was studied in rabbits given a 2% cholesterol diet. Twenty four New Zealand White rabbits (2.2 – 2.8 kg) were divided into 3 groups. Group 1 (control) was fed a normal diet, group II (HC) was fed a 2% cholesterol diet and group III (HC+NICA) was fed a 2% cholesterol diet with nicardipine treatment (0.5mg kg−1 body weight twice daily intramuscularly for 10 weeks). The following parameters which included fasting plasma total cholesterol...

  11. Lipidomic analysis of plasma lipoprotein fractions in myocardial infarction-prone rabbits.

    Science.gov (United States)

    Takeda, Hiroaki; Koike, Tomonari; Izumi, Yoshihiro; Yamada, Takayuki; Yoshida, Masaru; Shiomi, Masashi; Fukusaki, Eiichiro; Bamba, Takeshi

    2015-10-01

    Lipids play important roles in the body and are transported to various tissues via lipoproteins. It is commonly assumed that alteration of lipid levels in lipoproteins leads to dyslipidemia and serious diseases such as coronary artery disease (CAD). However, lipid compositions in each lipoprotein fraction induced by lipoprotein metabolism are poorly understood. Lipidomics, which involves the comprehensive and quantitative analysis of lipids, is expected to provide valuable information regarding the pathogenic mechanism of CAD. Here, we performed a lipidomic analysis of plasma and its lipoprotein fractions in myocardial infarction-prone Watanabe heritable hyperlipidemic (WHHLMI) rabbits. In total, 172 lipids in plasma obtained from normal and WHHLMI rabbits were quantified with high throughput and accuracy using supercritical fluid chromatography hybrid quadrupole-Orbitrap mass spectrometry (SFC/Q-Orbitrap-MS). Plasma levels of each lipid class (i.e., phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, lysophosphatidylcholine, lysophosphatidylethanolamine, sphingomyelin, ceramide, triacylglycerol, diacylglycerol, and cholesterol ester, except for free fatty acids) in 21-month-old WHHLMI rabbits were significantly higher than those in normal rabbits. High levels of functional lipids, such as alkyl-phosphatidylcholines, phospholipids including ω-6 fatty acids, and plasmalogens, were also observed in WHHLMI rabbit plasma. In addition, high-resolution lipidomic analysis using very low density lipoprotein (VLDL) and low density lipoprotein (LDL) provided information on the specific molecular species of lipids in each lipoprotein fraction. In particular, higher levels of phosphatidylethanolamine plasmalogens were detected in LDL than in VLDL. Our lipidomics approach for plasma lipoprotein fractions will be useful for in-depth studies on the pathogenesis of CAD. Copyright © 2015 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights

  12. Sulfuriferula thiophila sp. nov., a chemolithoautotrophic sulfur-oxidizing bacterium, and correction of the name Sulfuriferula plumbophilusWatanabe, Kojima and Fukui 2015 to Sulfuriferula plumbiphila corrig.

    Science.gov (United States)

    Watanabe, Tomohiro; Kojima, Hisaya; Fukui, Manabu

    2016-05-01

    A novel sulfur-oxidizing bacterium designated strain mst6T was isolated from spring water of Masutomi hot spring in Japan. The cells were rod-shaped (1.2-4.0 × 0.5-0.7 μm) and Gram-stain-negative. The G+C content of genomic DNA was around 52.6 mol%. The isolate possessed summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 and C12 : 0 as major cellular fatty acids. Strain mst6T grew by inorganic carbon fixation and oxidation of inorganic sulfur compounds with oxygen as an electron acceptor. The isolate grew over a temperature range of 5-34 °C, a NaCl concentration range of 0-110 mM and a pH range of 4.6-8.1. Optimum growth occurred at 32 °C, in the absence of NaCl and at pH 5.9-6.2. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain mst6T belongs to the family Sulfuricellaceae in the class Betaproteobacteria. The closest cultured relative was Sulfuriferula multivorans TTNT with a 16S rRNA gene sequence similarity of 97.0 %. On the basis of the data obtained in this study, strain mst6T represents a novel species of the genus Sulfuriferula, for which the name Sulfuriferula thiophila sp. nov. is proposed. The type strain is mst6T ( = NBRC 111150T = DSM 101871T). In addition, we propose correcting the name Sulfuriferula plumbophilus Watanabe, Kojima and Fukui 2015 to Sulfuriferula plumbiphila corrig. based on Rule 12c, Rule 61 and Appendix 9 of the International Code of Nomenclature of Prokaryotes.

  13. Evaluation of the use of serum lathosterol concentration to assess whole-body cholesterol synthesis in rabbits

    NARCIS (Netherlands)

    Meijer, G.W.; Palen, J.G. van der; Vries, H. de; Kempen, H.J.; Voort, H.A. van der; Zutphen, L.F. van; Beynen, A.C.

    1992-01-01

    Serum lathosterol concentration in rabbits was assessed as a possible indicator of whole-body cholesterol synthesis. In random-bred New Zealand White (NZW) rabbits fed a control diet or a diet containing either cholesterol, simvastatin, or cholestyramine, neither serum lathosterol concentration nor

  14. Autoantibody Production in Rabbits

    Science.gov (United States)

    Asherson, G. L.; Rose, M. Elaine

    1963-01-01

    The finding that the serum of apparently healthy rabbits fixed complement with rabbit liver and kidney has been confirmed. Experimental infection of rabbits with Eimeria stiedae, the cause of hepatic coccidiosis, led to a rise in the titre of serum complement-fixing factors. The rise was statistically significant 14, 21 and 28 days after infection. The factors were regarded as antibodies because they behaved as macroglobulins on diethylaminoethyl—cellulose chromatography and sucrose gradient centrifugation, and as autoantibodies because they fixed complement with the kidney of the rabbits in which they occurred. The antibody reacted with widely distributed antigen(s) with high activity in brain and low activity in skeletal muscle. The possibility that coccidial infection may be responsible for the natural autoantibody of rabbits is discussed. PMID:13965167

  15. Influence of Hyperlipidemia on the Proliferation and Apoptosis of Myocardial Cells in Rabbits

    Directory of Open Access Journals (Sweden)

    Huse Kinscherf

    2015-03-01

    Full Text Available Objective: To establish hyperlipemia models in rabbits fed with high fat diet, observe the changes of serum lipid level, and to explore the effect of hyperlipidemia on the proliferation and apoptosis of myocardial cells in rabbits. Methods: New Zealand white male special rabbits were randomly divided into 2 groups: normal diet group and high-fat-fed group, 10 cases in each group. The rabbits in normal group were fed with ordinary feed while the rabbits in the latter group were fed with high fat diet. The levels of serum TC, TG, HDL-C and LDL-C were detected at 0, 4th, 7th, 10th, 13thweeks for 2 groups. At the end of the 13th week, the pathological changes of myocardial tissues were detected by HE staining, and the proliferation and apoptosis of cardiomyocyte were tested by FCM. Results:After given high fat diet, the level of serum TC, TG, HDL-C and LDL-C in high-fat-fed diet group were significantly increased than in the normal diet group from the 4th week to the 13th week (P<0.01. The construction of cardiomyocyte in the atherogenic diet group had obviously morphological changes. The distribution of G0/G1 phase in high-fat-fed increased significantly than that in the normal diet group (P<0.01, but S phase and proliferation index (PI in decreased significantly than that in the normal diet group (P<0.01. The apoptosis rate in both groups had no statistical difference (P>0.05. Conclusion: Hyperlipidemia can induce the pathological changes of cardiomyocyte in rabbits, inhibit the proliferation of cardiomyocyte, but has no effect on the apoptosis of cardiomycyte in rabbits.

  16. Zoonoses of rabbits and rodents.

    Science.gov (United States)

    Hill, William Allen; Brown, Julie Paige

    2011-09-01

    Millions of households in the US own rabbits or rodents, including hamsters, guinea pigs, and gerbils. Activities such as hunting and camping also involve human interactions with wild rabbits and rodents. In many environments, feral rabbits and rodents live in close proximity to humans, domesticated animals, and other wildlife. Education of rodent and rabbit owners and individuals with occupational or recreational exposures to these species is paramount to reduce the prevalence of zoonoses associated with rabbit and rodent exposure.

  17. Rabbit orthopedic surgery.

    Science.gov (United States)

    Rich, Gregory A

    2002-01-01

    Orthopedic surgery in rabbits poses several unique parameters for the veterinary surgeon. It is imperative for the veterinarian to be knowledgeable about the anatomic features of the surgical repair site and to become familiar with a rabbit's pain and discomfort often associated with orthopedic injuries. Handling the perioperative and postoperative pain and potential GI disturbances are crucial for a successful outcome of the surgical case. This article is designed to help the veterinary surgeon prepare for the orthopedic surgical procedure and the peripheral physiologic needs of the rabbit from presentation through recovery.

  18. RabbitMQ essentials

    CERN Document Server

    Dossot, David

    2014-01-01

    This book is a quick and concise introduction to RabbitMQ. Follow the unique case study of Clever Coney Media as they progressively discover how to fully utilize RabbitMQ, containing clever examples and detailed explanations.Whether you are someone who develops enterprise messaging products professionally or a hobbyist who is already familiar with open source Message Queuing software and you are looking for a new challenge, then this is the book for you. Although you should be familiar with Java, Ruby, and Python to get the most out of the examples, RabbitMQ Essentials will give you the push y

  19. Selective ablation of WHHLMI rabbit atherosclerotic plaque by quantum cascade laser in the 5.7 μm wavelength range for less-invasive laser angioplasty

    Science.gov (United States)

    Hashimura, Keisuke; Ishii, Katsunori; Akikusa, Naota; Edamura, Tadataka; Yoshida, Harumasa; Awazu, Kunio

    2013-06-01

    We investigated the potential of a compact and high-power quantum cascade laser (QCL) in the 5.7 μm wavelength range for less-invasive laser angioplasty. Atherosclerotic plaques consist mainly of cholesteryl esters. Radiation at a wavelength of 5.75 μm is strongly absorbed in C=O stretching vibration mode of cholesteryl esters. Our previous study achieved to make cutting differences between a normal artery and an atherosclerotic lesions using nanosecond pulsed laser by difference-frequency generation (DFG laser) at the wavelength of 5.75 μm. For applying this technique to clinical treatment, a compact laser device is required. In this study, QCL irradiation effects to a porcine normal aorta were compared with DFG laser. Subsequently, QCL irradiation effects on an atherosclerotic aorta of myocardial infarction-prone Watanabe heritable hyperlipidemic rabbit (WHHLMI rabbit) and a normal rabbit aorta were observed. As a result, the QCL could make cutting differences between the rabbit atherosclerotic and normal aortas. On the other hand, the QCL induced more thermal damage to porcine normal aorta than the DFG laser at the irradiation condition of comparable ablation depths. In conclusion, the possibility of less-invasive and selective treatment of atherosclerotic plaques using the QCL in the 5.7 μm wavelength range was revealed, although improvement of QCL was required to prevent the thermal damage of a normal artery.

  20. Rabbit dietary supplementation with pale purple coneflower. 2. Effects on the performances, bacterial community, blood parameters and immunity of growing rabbits.

    Science.gov (United States)

    Kovitvadhi, A; Gai, F; Dabbou, S; Ferrocino, I; Rotolo, L; Falzone, M; Vignolini, C; Gennero, M S; Bergagna, S; Dezzutto, Daniela; Barbero, R; Nebbia, P; Rosati, S; Cocolin, L; Zoccarato, I; Gasco, L

    2016-07-01

    Echinacea pallida (EPAL), a herbaceous flowering plant with immunomodulatory properties, has been chosen to determine the pre- and post-supplementary effects on the growth performances, bacterial community, blood parameters and immunity of growing rabbits. The same Grimaud does (14-week-old) from the studied in the first part of this study were randomly divided into two groups (n=50/group). The first group was fed a basal diet without supplementation (Control group, C) while the another group was fed a basal diet supplemented with 3 g EPAL/kg diet (Echinacea group, E). From the second parturition, 80 weaned kits (40 from the C does and 40 from the E does) were randomly assigned to four groups of 20 animals each and were fed a growing commercial diet supplemented with or without a 3 g EPAL/kg diet: the CC group (rabbits from the C does fed the control diet), CE group (rabbits from the C does fed the supplemented diet), EC (rabbits from the E does fed the control diet) and EE group (rabbits from the E does fed the supplemented diet). The dietary EPAL treatment did not affect the growth performance. Ten fattening rabbits from each group were selected to evaluate the bacterial community and blood parameters, while the remaining rabbits (n=10/group) were used to study phagocytosis and the humoral immune response. The variability was evaluated from hard faeces at 35, 49 and 89 days, and the caecal content at 89 days. The variability of the bacterial community of the EE group was higher than that of the other groups. The phagocytic activity was higher in the CE and EE groups than in the CC and EC ones (30.9 and 29.7 v. 21.2 and 21.8%; Pold which the serum was collected at 88, 102, 109, 116 and 123 days old. In conclusion, no impact of EPAL dietary supplementation has been observed on the growth performances, bacterial community, blood parameters or humoral immune responses in growing rabbits, except for an increase in phagocytic activities.

  1. Milkweed control by food imprinted rabbits.

    Science.gov (United States)

    Ducs, Anita; Kazi, Andrea; Bilkó, Ágnes; Altbäcker, Vilmos

    2016-09-01

    Many species of invasive plants are spreading out rapidly in Europe. The common milkweed occupies increasingly more area. Being poisonous, most animals will not graze on it however rabbits would be an effective organism for the biological control of milkweed. Rabbit kittens can learn the maternal diet in various ways. They prefer aromatic foods which their mother had eaten during pregnancy or lactation period, -even if it is poisonous- but they can also learn the maternal diet from the fecal pellets deposited by the mother into the nest during the nursing events. The present study was aimed to investigate if rabbit kittens can learn that the common milkweed is a potential food also. In the first 10days of their lives kits got fecal pellets originating from individuals having fed on common milkweed previously. When weaned on day 28 postpartum, these pups preferred the milkweed in the 3-way food choice test, opposite to the control group. Most surprisingly in a second experiment it was also shown that the common milkweed was also preferred by the kittens if their mother ate it not during, but one month before pregnancy. Copyright © 2016 Elsevier B.V. All rights reserved.

  2. Endothelial Dysfunction in Experimental Atherosclerosis in the Rabbit with Extraction of Instantaneous Changes in the Arterial Wall

    Directory of Open Access Journals (Sweden)

    Tavoos Rahmani-Cherati

    2015-10-01

    Full Text Available Background: In this study, we used a new computerized analytical method for the measurement of the endothelial function in sequential ultrasound images and compared it with histological studies, using the abdominal aorta in normal and atherosclerotic rabbits.Methods: Six rabbits received a standard rabbit chow as the normal group and the other 6 rabbits were fed a high cholesterol diet for four weeks as the atherosclerotic group. B-mode images of the abdominal aorta with 46 frames per second were saved over three cardiac cycles at baseline and during acetylcholine or nitroglycerin drug infusion in the normal and atherosclerotic rabbits. In order to evaluate endothelial-dependent relaxation, acetylcholine-mediated dilation (AMD was measured during the infusion of acetylcholine at a rate of 0.5 µg/kg/min and endothelial-independent relaxation was evaluated by measuring nitroglycerine-mediated dilation (NMD during the infusion of nitroglycerin at a rate of 5 µg/kg/min. In addition, the ultrasonic evaluation was confirmed by histopathological evaluation of the abdominal aorta.Results: Significant differences in AMD were detected between the normal and the four-week cholesterol-fed rabbits (p value < 0.05, whereas there were no significant differences in NMD between the two groups (p value > 0.05. No microscopic intimal lesions were seen in the normal rabbits, but intimal thickening was observed in the histological studies in the four- week cholesterol-fed rabbits. Additionally, the total cholesterol, triglycerides, low-density lipoprotein cholesterol, and high- density lipoprotein cholesterol levels were remarkably increased in the sera of the four-week cholesterol-fed rabbits (p value< 0.05.Conclusion: A new automatic method can help accurately evaluate the endothelial function in normal and hypercholesterolemic rabbits.

  3. Viral diseases of the rabbit.

    Science.gov (United States)

    Krogstad, Aric P; Simpson, Janet E; Korte, Scott W

    2005-01-01

    Viral disease in the rabbit is encountered infrequently by the clinical practitioner; however, several viral diseases were reported to occur in this species. Viral diseases that are described in the rabbit primarily may affect the integument, gastrointestinal tract or, central nervous system or maybe multi-systemic in nature. Rabbit viral diseases range from oral papillomatosis, with benign clinical signs, to rabbit hemorrhagic disease and myxomatosis, which may result in significant clinical disease and mortality. The wild rabbit may serve as a reservoir for disease transmission for many of these viral agents. In general, treatment of viral disease in the rabbit is supportive in nature.

  4. Experimental infections with Fasciola in snails, mice and rabbits.

    Science.gov (United States)

    Hussein, Abdel-Nasser A; Khalifa, R M A

    2008-05-01

    Experimental infection trails of Lymnaea (cailliaudi) natalensis snails with miracidia of Fasciola hepatica revealed neither cercariae nor larval stages shed. Infection of white mice with metacercariae from field-collected snails proved to be negative for Fasciola eggs and immature juveniles or adults after 84 days post infection. The infection of eight rabbits (Oryctolagus cuniculus) has succeeded; two rabbits were infected, with a very low infection rate. Faeces of rabbits were negative for eggs. The worm burden was one and three worms from 40 fed metacercariae. The obtained fluke measures 23 mm in length by 4 mm in width. The tegument is covered with sharp-ending spines. The uterus contains few eggs. The intrauterine eggs measured 158 microm x 80 microm. According to the morphological characters of these flukes, they belong to F. gigantica.

  5. [The inhibitory effects of antioxidant vitamins on serum oxLDL and experimental atherosclerosis of rabbits].

    Science.gov (United States)

    Chen, D; Yang, T; Song, F; Ruan, G; Liu, S

    1997-12-01

    In order to study the inhibitory effects of antioxidant vitamins on serum (low oxidative density lipoproteins, oxLDL) and experimental atherosclerosis in rabbits, 20 rabbits were fed on cholesterol rich diet and antioxidant vitamins (vitamin E, vitamin C and beta carotene) for 12 weeks. oxLDL were tested by ELISA at the beginning of experiment and after 4 weeks 8 weeks. The results showed that supplement of antioxidant vitamins can decrease the oxLDL level significantly and inhibited development of atherosclerosis lesion around aorta in rabbits.

  6. Effect of Different Rearing Methods on the Weight of Rabbits

    Directory of Open Access Journals (Sweden)

    Mónika Gyovai

    Full Text Available The aim of the experiment was to study the effect of the nutrient supply at foetal, suckling and growing age on the body weight of rabbits. 242 new-born rabbits were divided into three groups on the basis of their birth weight (Low = 34-45 g, Medium = 53-58 g, High = 65-70 g. One half of the litters were nursed by one doe, while the other half by two does. After weaning at 21 days of age, half of the rabbits were fed ad libitum, while the other half were fed restricted (ca. 85-90% of the ad lib.. All of the 12 groups were divided into two subgroups randomly: first artificial insemination (AI at 15.5 or 18.5 weeks of age. All of the studied factors had significant effects on the body weight of rabbits. At four weeks of age the influence of birth weight was the highest (L: 0.51, H: 0.67 kg, P<0.05. A significant difference was found between the groups nursed by one or two does (0.52 and 0.64 kg. At nine weeks of age the effect of all factors were similar. At 15 weeks of age the following differences were found: L: 3.03, H: 3.37 kg, one or two does: 3.11 and 3.26 kg, rest. and ad lib.: 3.00 and 3.37 kg, respectively. The highest difference was found between the group of low birth weight, nursed by one doe and fed restricted (2.75 kg and that of high birth weight, nursed by two does and fed ad libitum (3.81 kg.

  7. Effect of feed restriction and different crude protein sources on the performance, health status and carcass traits of growing rabbits

    Directory of Open Access Journals (Sweden)

    L. Uhlířová

    2015-12-01

    Full Text Available We evaluate the effect of the crude protein source (CP in combination with 2 feeding strategies (AL: ad libitum or R: restricted on the performance, health status and carcass traits of growing-fattening rabbits (between 33 and 75 d of age. Two experimental diets were formulated; the first containing soybean meal (SBM: 70 g/kg as the main CP source and the second containing white lupin seeds (WLS: 105 g/kg as the main CP source. A total of 160 weaned Hyplus rabbits (774±10 g live weight were randomly allocated to the experimental groups, combination of feeding strategy and diet (AL SBM, R SBM, AL WLS and R WLS; 40 rabbits per group with 4 rabbits per cage, for 42 d. The feeding programme was applied as follows: both the AL SBM and AL WLS rabbits were fed ad libitum for the entire fattening period, whereas the R SBM and R WLS rabbits were subjected to feed restriction between 33 and 47 d of age (75% of the ad libitum intake. Afterwards, all restricted rabbits were fed ad libitum until 75 d of age. Regardless of the CP source, the feed restriction reduced the final live weight by 83 g, chilled carcass weight by 65 g, and dressing out percentage by 0.9 percentage points (P<0.05. There was a higher chilled carcass weight (+114 g; P=0.001 and reference carcass weight (+91 g; P=0.001 in rabbits fed with WLS diet than in rabbits fed with SBM diet. No dead or morbid rabbits were observed in restricted rabbits during the restriction period. There was no effect of the diet on the health of rabbits. However when the rabbits of AL SBM and AL WLS group were compared did we observe a lower number of rabbits at sanitary risk in AL WLS group (2 vs. 12 rabbits, P=0.006. In conclusion, the growth performance was not affected by the crude protein source, and no interaction between dietary CP source and feeding regime was observed. Feed restriction regime did not improve sanitary risk index throughout the entire period.

  8. The usefulness of optical analyses for detecting vulnerable plaques using rabbit models

    Science.gov (United States)

    Nakai, Kanji; Ishihara, Miya; Kawauchi, Satoko; Shiomi, Masashi; Kikuchi, Makoto; Kaji, Tatsumi

    2011-03-01

    Purpose: Carotid artery stenting (CAS) has become a widely used option for treatment of carotid stenosis. Although technical improvements have led to a decrease in complications related to CAS, distal embolism continues to be a problem. The purpose of this research was to investigate the usefulness of optical methods (Time-Resolved Laser- Induced Fluorescence Spectroscopy [TR-LIFS] and reflection spectroscopy [RS] as diagnostic tools for assessment of vulnerable atherosclerotic lesions, using rabbit models of vulnerable plaque. Materials & Methods: Male Japanese white rabbits were divided into a high cholesterol diet group and a normal diet group. In addition, we used a Watanabe heritable hyperlipidemic (WHHL) rabbit, because we confirmed the reliability of our animal model for this study. Experiment 1: TR-LIFS. Fluorescence was induced using the third harmonic wave of a Q switch Nd:YAG laser. The TR-LIFS was performed using a photonic multi-channel analyzer with ICCD (wavelength range, 200 - 860 nm). Experiment 2: RS. Refection spectra in the wavelength range of 900 to 1700 nm were acquired using a spectrometer. Results: In the TR-LIFS, the wavelength at the peak was longer by plaque formation. The TR-LIFS method revealed a difference in peak levels between a normal aorta and a lipid-rich aorta. The RS method showed increased absorption from 1450 to 1500 nm for lipid-rich plaques. We observed absorption around 1200 nm due to lipid only in the WHHL group. Conclusion: These methods using optical analysis might be useful for diagnosis of vulnerable plaques. Keywords: Carotid artery stenting, vulnerable plaque, Time-Resolved Laser-Induced Fluorescence

  9. INFLUENCE OF NATURAL IMMUNOMODULATORS ON PROTEIN FRACTIONS AND CORTISOL CONTENT IN RABBIT BLOOD UNDER STRESS

    Directory of Open Access Journals (Sweden)

    Grabovskyi S.

    2015-08-01

    Full Text Available The results of determination of protein fractions, cortisol content in blood of rabbits, which further added to the feed of natural origin biologically active substances are presented in the article. As an antistressors and immunomodulators in pre-slaughter period are using of spleen extract biologically active substances were obtained with ultrasound application. The purpose of research — determination of changes of protein fractions, cortisol content in rabbits blood before slaughter and their correction of natural origin biologically active substances (spleen extract. Object and research methods. The experiment was conducted on 15 rabbits with standard diet. Three groups of rabbits five month of age (5 rabbits each was formed for research. The spleen extract were using as an biologically active substances to the feed rabbits in pre-slaughter period (five days before slaughter. The extracts were applied to feed by aerosol method (70 °alcohol solution of spleen extract volume of 1.4 ml per rabbit (group I. The rabbits (group II received to the feed in the same way of 70 °alcohol solution in the same volume. The control group rabbits received the standard feed in the same volume. The feed eating by rabbits was exercised daily. The rabbits ate food completely. The rabbits slaughter was carried out in the morning. The blood plasma protein fractions separation was carried out by horizontal electrophoresis in polyacrylamide gel (PAAG. Mathematical treatment of the research results worked statistically using the software package Statistica 6.0 and Microsoft Excel for Windows XP. Probability differences was assessed by Student t-test and results considered likely at P ≤ 0.05. Results and discussion. We measured the ratio of blood plasma protein fractions of rabbits, which in addition to the feed fed of natural origin biologically active substances. As a result of research was found that aerosol introduction of the spleen extract to the rabbits

  10. [Effect of vitamin E on myosin light chain kinase activity and endothelial permeability of the artery in atherosclerotic rabbit].

    Science.gov (United States)

    Zhu, Hua-qing; Ren, Bin; Xiong, Jiang-xia; Hu, Ruo-lei; Liu, Xiao-ying; Wang, Xue; Zhang, Su-mei; Liu, Hui-lan; Jiang, Zhi-kui; Zhou, Qing; Gui, Shu-yu; Wang, Yuan

    2003-08-01

    To study the effect of vitamin E (Vit E) on the myosin light chain kinase(MLCK) activity and the endothelial permeability of the artery in atherosclerotic rabbits. The MLCK activity of rabbit artery was measured by incorporation of gamma-(32)P. The endothelial permeability was accessed by immunofluorescence. The model of atherosclerosis was established after rabbits were fed with cholesterol for four weeks. The activity of MLCK increased markedly, and there was significantly statistical difference compared with the normal control (Prabbits were fed with cholesterol for twelve weeks or with cholesterol and Vit E for twelve weeks, the activity of MLCK did not change markedly, and there was no statistical difference compared with the normal control, respectively (P>0.05). The permeability of arterial wall was increased after the rabbits were fed with cholesterol for four weeks, and the permeability increased even more obviously after the rabbits were fed with cholesterol for twelve weeks. The permeability appeared to be decreased when Vit E was added into the cholesterol feeding. The change in integrity of arterial wall may be associated with the increase of the activity of MLCK. Vit E may decrease the MLCK activity. Vit E may decrease the endothelial permeability of atherosclerotic rabbits.

  11. Hepatitis E Virus in Farmed Rabbits, Wild Rabbits and Petting Farm Rabbits in the Netherlands

    NARCIS (Netherlands)

    Burt, S.A.; Veltman, Jorg; Hakze-van der Honing, Renate; Schmitt, Heike; van der Poel, Wim H.M.

    2016-01-01

    Rabbits have been suggested as a zoonotic source of Hepatitis E virus. Phylogenetic analysis of HEV isolates from farmed, wild and pet rabbits in the Netherlands (23, 0, and 60 % respectively) showed them to be grouped amongst published rabbit HEV sequences and distinct from most human isolates. Dut

  12. Hepatitis E Virus in Farmed Rabbits, Wild Rabbits and Petting Farm Rabbits in the Netherlands

    NARCIS (Netherlands)

    Burt, Sara A.; Veltman, Jorg; Hakze-van der Honing, Renate; Schmitt, Heike; Poel, van der Wim H.M.

    2016-01-01

    Rabbits have been suggested as a zoonotic source of Hepatitis E virus. Phylogenetic analysis of HEV isolates from farmed, wild and pet rabbits in the Netherlands (23, 0, and 60 % respectively) showed them to be grouped amongst published rabbit HEV sequences and distinct from most human isolates.

  13. Hepatitis E Virus in Farmed Rabbits, Wild Rabbits and Petting Farm Rabbits in the Netherlands

    NARCIS (Netherlands)

    Burt, Sara A.; Veltman, Jorg; Hakze-van der Honing, Renate; Schmitt, Heike; Poel, van der Wim H.M.

    2016-01-01

    Rabbits have been suggested as a zoonotic source of Hepatitis E virus. Phylogenetic analysis of HEV isolates from farmed, wild and pet rabbits in the Netherlands (23, 0, and 60 % respectively) showed them to be grouped amongst published rabbit HEV sequences and distinct from most human isolates.

  14. Hepatitis E Virus in Farmed Rabbits, Wild Rabbits and Petting Farm Rabbits in the Netherlands

    NARCIS (Netherlands)

    Burt, S.A.|info:eu-repo/dai/nl/140114432; Veltman, Jorg; Hakze-van der Honing, Renate; Schmitt, Heike; van der Poel, Wim H.M.

    2016-01-01

    Rabbits have been suggested as a zoonotic source of Hepatitis E virus. Phylogenetic analysis of HEV isolates from farmed, wild and pet rabbits in the Netherlands (23, 0, and 60 % respectively) showed them to be grouped amongst published rabbit HEV sequences and distinct from most human isolates.

  15. A Clever Rabbit

    Institute of Scientific and Technical Information of China (English)

    呼振璞; 付晓明

    2002-01-01

    1. Once there lived an elephant and a lion in the mountains, who both wanted to be the king of the beasts. One day the lion caught a rabbit. Before he ate it, he said, “Do you know I am the king here? ”

  16. Rabbit Repellent Paint

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Five gallons of rabbit repellent paint were sent to George Wilson to be applied on the trees of the Tewaukon tree plot. Mr. Wilson requires a 3 or 4 in. brush for...

  17. The Cutaneous Rabbit Revisited

    Science.gov (United States)

    Flach, Rudiger; Haggard, Patrick

    2006-01-01

    In the cutaneous rabbit effect (CRE), a tactile event (so-called attractee tap) is mislocalized toward an adjacent attractor tap. The effect depends on the time interval between the taps. The authors delivered sequences of taps to the forearm and asked participants to report the location of one of the taps. The authors replicated the original CRE…

  18. Can an Antibiotic (Macrolide) Prevent Chlamydia pneumoniae-Induced Atherosclerosis in a Rabbit Model?

    Science.gov (United States)

    Fong, Ignatius W.; Chiu, Brian; Viira, Esther; Jang, Dan; Fong, Michael W.; Peeling, Rosanna; Mahony, James B.

    1999-01-01

    There is increasing data implicating Chlamydia pneumoniae in the pathogenesis of atherosclerosis, and antibiotics may theoretically be useful to prevent secondary vascular complications. Three groups of New Zealand White specific-pathogen-free rabbits, fed cholesterol-free chow, were inoculated via the nasopharynx on three occasions, 2 weeks apart, with C. pneumoniae. Group I (n = 23) rabbits were untreated; group II (n = 24) rabbits were treated with azithromycin at 30 mg/kg of body weight daily for 3 days and then once every 6 days, starting 5 days after first inoculation and continuing until sacrifice (early treatment); and group III (n = 24) rabbits were treated with the same dose of azithromycin but initiated 2 weeks after the last inoculation. All animals were sacrificed at 10 to 11 weeks after initial inoculation and examined for signs of atherosclerosis of the aorta. Eight (34.8%) untreated rabbits developed early signs of atherosclerosis, whereas only one (4.2%) in the early-treatment group had such signs (P = 0.02). However, eight rabbits (33.3%) of the delayed-treatment group had atherosclerotic changes of the aorta and no significant reduction compared to untreated rabbits. Early treatment of C. pneumoniae-infected rabbits with azithromycin was highly effective (87%) in preventing atherosclerotic changes, but delayed treatment was ineffective. It is possible that longer or more aggressive antibiotic treatment may be needed to reverse preformed lesions or that antibiotics may not be of value once lesions have formed. PMID:10548582

  19. Atividade da amilase no intestino delgado de coelhos alimentados com dietas contendo diferentes níveis de amido antes e após desmame = Amylase activity in the small intestine of rabbits fed with diets containing starch levels before and after weaning

    Directory of Open Access Journals (Sweden)

    Haroldo Garcia de Faria

    2006-07-01

    Full Text Available Para avaliar a atividade amilásica em coelhos após a desmama, foram utilizados 200 animais da Raça Nova Zelândia Branco, submetidos a diferentes padrões de alimentação desde a fase de lactação. Quarenta deles foram abatidos aos 35 dias de idade e 80, aos 50 e aos 70 dias de idade. Para modular o padrão de consumo de leite e ração antes da desmama, as ninhadas equalizadas em 8 láparos do nascimento até ao 16º dia de idade, a partir desta idade, até a desmama, foram submetidos à alta e baixa disponibilidade de leite, mantendo ninhadas com 4 ou 8 láparos recebendo dietas com nível normal (15% e alto (24% de amido. A partir da desmama, os animais de cada um dos 4 padrões de alimentação até a desmama passaram a receber as mesmasdietas com níveis normal e alto de amido, em um esquema fatorial 2x2x2 (ninhadas submetidas à baixa e alta disponibilidade de leite versus dois níveis de amido na dieta até a desmama versus doisníveis de amido na dieta após a desmama. Os resultados mostraram que a manutenção de ninhadas com 4 ou 8 animais entre o 16º e 35º dia de lactação não modificou (P>0,05 a atividade da amilase em qualquer das idades avaliadas após o desmame. O uso de dietas com alto teor de amido durante a lactação aumentou (POne experiment was carried out to study the amylase activity in the small intestine (jejunum and ileum of rabbits from litters maintained on different feeding patterns. Two hundred White New Zealand rabbits were analyzed, out of which 40 35- days-old rabbits, 80 50-days-old rabbits and 80 70-days-old rabbits were slaughtered. To modulate the pattern of feed and milk intake before weaning, the litters were modulated for eight young rabbits until 16 days old. From 16 days until weaning, they were submitted to low or high milkavailability, litters maintained with four or eight young rabbits receiving diets containing normal or high starch level. The animals were distributed in a factorial design 2

  20. Feline immunodeficiency virus and retrovirus-mediated adventitial ex vivo gene transfer to rabbit carotid artery using autologous vascular smooth muscle cells.

    Science.gov (United States)

    Kankkonen, Hanna M; Turunen, Mikko P; Hiltunen, Mikko O; Lehtolainen, Pauliina; Koponen, Jonna; Leppänen, Pia; Turunen, Anna-Mari; Ylä-Herttuala, Seppo

    2004-03-01

    We have developed an ex vivo gene transfer technique to rabbit arterial wall using autologous smooth muscle cells (SMCs). SMCs were harvested from rabbit ear artery, transduced in vitro with vesicular stomatitis virus G-glycoprotein pseudotyped retrovirus or feline immunodeficiency virus (FIV) and returned to the adventitial surface of the carotid artery using a periadventitial silicone collar or collagen sheet placed around the artery. Beta-galactosidase (lacZ) and human apolipoprotein E3 (apoE3) cDNAs were used as transgenes. After retrovirus-mediated gene transfer of lacZ the selected cells implanted with high efficiency and expressed lacZ marker gene at a very high level 7 and 14 days after the operation. The level of lacZ expression decreased thereafter but was still detectable 12 weeks after the gene transfer, and was exclusively localized to the site of cell implantation inside the collar. Utilizing FIV vector expressing apoE3, low levels of apoE were measured from serum collected from a low-density lipoprotein receptor deficient Watanabe heritable hyperlipidemic rabbits 1 month after the gene transfer. The physiological effect of apoE expression was detected as transiently elevated serum cholesterol levels. The results indicate that the model can be used for high efficiency local gene transfer in arteries, e.g. during vascular surgery. The model is also valuable for studying expression, stability and safety of new gene transfer vectors and their expression products in vivo.

  1. l-citrulline and l-arginine supplementation retards the progression of high-cholesterol-diet-induced atherosclerosis in rabbits

    OpenAIRE

    Hayashi, Toshio; Juliet, Packiasamy A.R.; Matsui-Hirai, Hisako; Miyazaki, Asaka; Fukatsu, Akiko; Funami, Jun; Iguchi, Akihisa; Ignarro, Louis J

    2005-01-01

    The objective of this study was to evaluate the influence of ingested l-arginine, l-citrulline, and antioxidants (vitamins C and E) on the progression of atherosclerosis in rabbits fed a high-cholesterol diet. The fatty diet caused a marked impairment of endothelium-dependent vasorelaxation in isolated thoracic aorta and blood flow in rabbit ear artery in vivo, the development of atheromatous lesions and increased superoxide anion production in thoracic aorta, and increased oxidation-sensitiv...

  2. The Investigation of the Effects of Pistacia terebinthus L. Upon Experimentally Induced Hypercholesterolemia and Atherosclerosis in Rabbits

    OpenAIRE

    BAKIREL, Tülay; ŞENER, Süleyman

    2003-01-01

    The possible pharmacological and toxicological effects of Pistacia terebinthus L. in experimentally hypercholesterolemic rabbits were investigated in this study. A total of 28 rabbits were divided into four groups, and these groups were fed diets containing standard pellets (Group 1), cholesterol (2%) (Group II), P. terebinthus L. (1 g/kg body weight) and cholesterol (2%) (Group III) and P. terebinthus L. (1 g/kg body weight) (Group IV) for 14 weeks. When the data were statistically evalua...

  3. Laparoscopic ovariectomy in rabbits

    Directory of Open Access Journals (Sweden)

    M. S. Al-Badrany

    2009-01-01

    Full Text Available A comparative evaluation of three different techniques of laparoscopic ovariectomy was carried out in 33 healthy female in rabbits, which included resection and removal of ovary after clip application, electrocautery of the ovary, then resection, and pulling ovary outside abdomen, ligation by silk, then ovary was removed. The ovaries and associated structures were better visualized by laparoscopy and all three techniques were carried out perfectly. All rabbits after operation were healthy and they were monitored for one month after operation. However, 3 of them died after operation, two of them died due to bleeding and the other of them died due to unknown causes. General anesthesia by using ketamine-xylazine i.m., was suitable for this technique, and the anesthesia provided good analgesia and good muscle relaxation. CO2 was used to establish pneumoperitoneum. In conclusion, resection and removal of the ovaries after clip application technique was found superior to the other two techniques.

  4. Lipidome of atherosclerotic plaques from hypercholesterolemic rabbits.

    Science.gov (United States)

    Bojic, Lazar A; McLaren, David G; Shah, Vinit; Previs, Stephen F; Johns, Douglas G; Castro-Perez, Jose M

    2014-12-15

    The cellular, macromolecular and neutral lipid composition of the atherosclerotic plaque has been extensively characterized. However, a comprehensive lipidomic analysis of the major lipid classes within atherosclerotic lesions has not been reported. The objective of this study was to produce a detailed framework of the lipids that comprise the atherosclerotic lesion of a widely used pre-clinical model of plaque progression. Male New Zealand White rabbits were administered regular chow supplemented with 0.5% cholesterol (HC) for 12 weeks to induce hypercholesterolemia and atherosclerosis. Our lipidomic analyses of plaques isolated from rabbits fed the HC diet, using ultra-performance liquid chromatography (UPLC) and high-resolution mass spectrometry, detected most of the major lipid classes including: Cholesteryl esters, triacylglycerols, phosphatidylcholines, sphingomyelins, diacylglycerols, fatty acids, phosphatidylserines, lysophosphatidylcholines, ceramides, phosphatidylglycerols, phosphatidylinositols and phosphatidylethanolamines. Given that cholesteryl esters, triacylglycerols and phosphatidylcholines comprise greater than 75% of total plasma lipids, we directed particular attention towards the qualitative and quantitative assessment of the fatty acid composition of these lipids. We additionally found that sphingomyelins were relatively abundant lipid class within lesions, and compared the abundance of sphingomyelins to their precursor phosphatidylcholines. The studies presented here are the first approach to a comprehensive characterization of the atherosclerotic plaque lipidome.

  5. Lipidome of Atherosclerotic Plaques from Hypercholesterolemic Rabbits

    Directory of Open Access Journals (Sweden)

    Lazar A. Bojic

    2014-12-01

    Full Text Available The cellular, macromolecular and neutral lipid composition of the atherosclerotic plaque has been extensively characterized. However, a comprehensive lipidomic analysis of the major lipid classes within atherosclerotic lesions has not been reported. The objective of this study was to produce a detailed framework of the lipids that comprise the atherosclerotic lesion of a widely used pre-clinical model of plaque progression. Male New Zealand White rabbits were administered regular chow supplemented with 0.5% cholesterol (HC for 12 weeks to induce hypercholesterolemia and atherosclerosis. Our lipidomic analyses of plaques isolated from rabbits fed the HC diet, using ultra-performance liquid chromatography (UPLC and high-resolution mass spectrometry, detected most of the major lipid classes including: Cholesteryl esters, triacylglycerols, phosphatidylcholines, sphingomyelins, diacylglycerols, fatty acids, phosphatidylserines, lysophosphatidylcholines, ceramides, phosphatidylglycerols, phosphatidylinositols and phosphatidylethanolamines. Given that cholesteryl esters, triacylglycerols and phosphatidylcholines comprise greater than 75% of total plasma lipids, we directed particular attention towards the qualitative and quantitative assessment of the fatty acid composition of these lipids. We additionally found that sphingomyelins were relatively abundant lipid class within lesions, and compared the abundance of sphingomyelins to their precursor phosphatidylcholines. The studies presented here are the first approach to a comprehensive characterization of the atherosclerotic plaque lipidome.

  6. The researchers developed luminous rabbit

    Institute of Scientific and Technical Information of China (English)

    2013-01-01

    <正>Their efforts produced two rabbits out of a litter of eight that went from being a normal, fluffy(蓬松的) white to glowing green in the dark. The rabbits were born at the University of Istanbul as part of a collaboration between scientists from universities in Turkey and Hawaii. The rabbits glow to show that a genetic manipulation technique can work efficiently,

  7. Oral administration of myostatin-specific recombinant Saccharomyces cerevisiae vaccine in rabbit.

    Science.gov (United States)

    Liu, Zhongtian; Zhou, Gang; Ren, Chonghua; Xu, Kun; Yan, Qiang; Li, Xinyi; Zhang, Tingting; Zhang, Zhiying

    2016-04-29

    Yeast is considered as a simple and cost-effective host for protein expression, and our previous studies have proved that Saccharomyces cerevisiae can deliver recombinant protein and DNA into mouse dendritic cells and can further induce immune responses as novel vaccines. In order to know whether similar immune responses can be induced in rabbit by oral administration of such recombinant S. cerevisiae vaccine, we orally fed the rabbits with heat-inactivated myostatin-recombinant S. cerevisiae for 5 weeks, and then myostatin-specific antibody in serum was detected successfully by western blotting and ELISA assay. The rabbits treated with myostatin-recombinant S. cerevisiae vaccine grew faster and their muscles were much heavier than that of the control group. As a common experimental animal and a meat livestock with great economic value, rabbit was proved to be the second animal species that have been successfully orally immunized by recombinant S. cerevisiae vaccine after mice.

  8. The Effect of Lactobacillus isolates on growth performance, immune response, intestinal bacterial community composition of growing Rex Rabbits.

    Science.gov (United States)

    Wang, C; Zhu, Y; Li, F; Huang, L

    2017-10-01

    The objective of this experiment was to investigate the effect of Lactobacillus isolates on intestinal bacterial community composition of growing Rex Rabbits. A total of 120 weaned Rex Rabbits (30 days old, 30 per group) were used for the experiment, which started after an adaptation period of 7 days. The control group was fed with basal diet only, while the treatment I, II and III groups were fed with basal diet adding antibiotics, Lactobacillus zeae (LB1) and Lactobacillus casei (L3) respectively. Growth performance, immune response and intestinal flora have been examined. The results obtained were as follows: (i) F/G of the rabbits fed with Lactobacillus isolates was significantly lower than that of the control group (p rabbits with Lactobacillus isolates. (iii) Lactobacillus isolates had no influence on the number of mast cells in duodenum and jejunum, but increased the number of mast cells in caecum significantly (p rabbit's intestinal flora can be changed by Lactobacillus isolates and antibiotics, especially for the microbial diversity and abundance in the caecum. In conclusion, the application of proper Lactobacillus isolates can improve the growth performance, enhance the immunological function and adjust the intestinal micro-ecosystem of growing Rex Rabbits. Journal of Animal Physiology and Animal Nutrition © 2017 Blackwell Verlag GmbH.

  9. The Effect of Dietary Linseed Oil and Organic Selenium on Growth Performance and Muscle Fatty Acids in Growing Rabbits

    OpenAIRE

    Ahmed A. Saleh, Tarek A. Ebeid and Yahya Z. Eid*

    2013-01-01

    The present study was conducted to evaluate the effect of a combined in-feed of linseed oil and organic selenium on growth performance and muscle fatty acid profiles in growing rabbits. A total of 4-week-old sixty male growing New Zealand white rabbits (average weight 531±5g) were collected and equally divided into 3 groups. The control group was fed on a control diet, whereas the treatment groups were fed on diets contained 2.5% linseed oil with or without 0.3 ppm organic selenium. All exper...

  10. Baccaurea angulata fruit juice ameliorates altered hematological and biochemical biomarkers in diet-induced hypercholesterolemic rabbits.

    Science.gov (United States)

    Ahmed, Idris Adewale; Mikail, Maryam Abimbola; Ibrahim, Muhammad

    2017-06-01

    Hypercholesterolemia is an important risk factor linked to the alteration of blood hematology and clinical chemistry associated with the development and progression of atherosclerosis. Previous studies have demonstrated the safety and potential health benefits of Baccaurea angulata (BA) fruit. We hypothesized that the oral administration of BA fruit juice could ameliorate the alteration in the hematological and biochemical biomarkers of diet-induced hypercholesterolemic rabbits. The aim of this study was to investigate the effects of different doses of BA juice on the hematological and biochemical biomarkers in normo- and hypercholesterolemic rabbits. Thirty-five healthy adult New Zealand White rabbits were assigned to seven different groups for 90days of diet intervention. Four atherogenic groups were fed a 1% cholesterol diet and 0, 0.5, 1.0, and 1.5mL of BA juice per kg of rabbit daily. The other three normal groups were fed a commercial rabbit pellet diet and 0, 0.5, and 1.0mL of BA juice per kg of rabbit daily. Baseline and final blood samples after 90days of repeated administration BA juice were analyzed for hematological parameters while serum, aortic and hepatic lysates were analyzed for lipid profiles and other biochemical biomarkers. The alteration of the hemopoietic system, physiological changes in serum and tissues lipid profiles and other biochemicals resulting from the consumption of a high-cholesterol diet were significantly (Pjuice. Improvements of the biomarkers in rabbits were dose-dependent, markedly enhanced at the highest dose of juice (1.5mL/kg/day). The results suggest potential health benefits of the antioxidant-rich BA fruit juice against hypercholesterolemia-associated hematological and biochemical alterations in the rabbit. Copyright © 2017 Elsevier Inc. All rights reserved.

  11. Ultrastructure of Reissner's membrane in the rabbit

    DEFF Research Database (Denmark)

    Qvortrup, K.; Rostgaard, Jørgen; Bretlau, P.

    1994-01-01

    Anatomy, Reissner's membrane, electron microscopy, tubulocisternal endoplasmic reticulum, subsurface cisterns, rabbit......Anatomy, Reissner's membrane, electron microscopy, tubulocisternal endoplasmic reticulum, subsurface cisterns, rabbit...

  12. Effects of aspirin on atherosclerosis and the cyclooxygenase-2 expression in atherosclerotic rabbits

    Institute of Scientific and Technical Information of China (English)

    GUO Yi; WANG Qi-zhang; TANG Bing-shan; ZUO Yan-fang; LI Fang-ming; JIANG Xin; WANG Ling; MA Ke-fu

    2006-01-01

    Background Atherosclerosis is a complex vascular inflammatory disease. Aspirin is a mainstay in the prevention of vascular complications of atherosclerosis. In this study, the effectiveness of aspirin in suppressing atherosclerosis and the inflammation process was evaluated in rabbits fed with a high fat diet.Methods Eighteen male New Zealand rabbits were randomly divided into 3 groups: control group, untreated cholesterol-fed group, aspirin treated cholesterol-fed group, which were fed for 12 weeks. After 12 weeks, the aorta was harvested for pathologic morphology observation. Immunohistochemical analysis of cyclooxygenase-2 (COX-2), macrophage and vascular smooth muscle cell (VSMC) was performed. The statistical analysis was performed by the statistical program SPSS 10.0.Results The aorta plaque/intima size (P/I) by pathologic morphology observation was 0%, (59.6± 13.7)% and (36.3± 16.5)% in the control, untreated cholesterol-fed group and aspirin treated group, respectively. The maximum plaque thickness, the degree of artery stenosis and the proportion of the intimal circumference occupied by atheroma of the 3 groups were significantly different from each other (P<0.01). The expression of COX-2 and macrophage in plaque of the aspirin treated group were decreased compared with that in untreated cholesterol-fed group. However, no difference was found in the expression of VSMC between the aspirin treated and the untreated cholesterol-fed group.Conclusion The mechanism of atherosclerosis suppression by aspirin in cholesterol-fed rabbits is related to the inhibition of COX-2 expression together with the reduced inflammation followed by, but not related to the hypolipidemic effects.

  13. Hypoglycemic and Hypolipidemic Potential of a High Fiber Diet in Healthy versus Diabetic Rabbits

    Directory of Open Access Journals (Sweden)

    Raquel Díez

    2013-01-01

    Full Text Available The aim of this study was to investigate potential hypoglycaemic and hypolipidemic effects of Plantago ovata husk included in the diet, in healthy and diabetic rabbits. We also examined the effects of this fiber in other biochemical parameters. Two groups of 18 rabbits were used. The first group was fed with standard chow and the second with chow supplemented with Plantago ovata husk (3.5 mg/kg/day. On day 14 diabetes mellitus was induced by the intravenous administration of alloxan (80 mg/kg. After an oral glucose load (3 g, glucose, insulin, and other biochemical parameters were determined on day 14 (healthy rabbits and on day 28 (diabetic rabbits. In healthy rabbits, fiber did not modify glucose or insulin levels but decreased significantly total cholesterol, LDL-cholesterol, atherogenic index, and glycosylated hemoglobin. In diabetic rabbits, fiber was more beneficial in mild diabetics than in severe diabetics with significant decreases in glucose levels and increases in insulin concentrations. In these animals fiber caused an important reduction in cholesterol, indicating a beneficial effect of Plantago ovata husk in diabetic rabbits. Although further studies in patients are necessary, we think that Plantago ovata husk offers interesting perspectives to be administered to patients with diabetes mellitus.

  14. The changes in various hydroxyproline fractions in aortic tissue of rabbits are closely related to the progression of atherosclerosis

    Directory of Open Access Journals (Sweden)

    Alhomida AS

    2010-03-01

    Full Text Available Abstract Background The most important function of collagen and elastin is to induce several mechanical parameters which are known to play a dominant role in governing mechanical properties of the blood vessels. The aortic tissue of rabbit is one of the important sources of collagen and elastin. The effects of high fat diet (HFD on the hydroxyproline (Hyp fractions in serum and aortic tissues of rabbits and collagen content in the aortic tissues of rabbits have not been documented before. The present study was undertaken to investigate the changes in Hyp fractions in serum and aortic tissues of rabbits and collagen content in the aortic tissues of rabbits during the progression of atherosclerosis. The atherosclerotic model used in this study was the New Zealand white rabbit (male; 12 weeks old. Twenty five rabbits were individually caged, and divided into control group (NOR; n = 10 and HFD group (CHO; n = 15. The control group was fed (100 g/day of normal (NOR diet for a period of 15 weeks. The HFD group was fed normal diet supplemented with 1.0% cholesterol plus 1.0% olive oil (100 g/day for the same period of time. Results We found that the TC, LDLC, and TG (mg/dl were significantly (p Conclusions These results suggest that percentage decrease in various Hyp fractions in aortic tissue of HFD rabbits are closely related to percentage decrease of collagen content in aortic tissues of HFD rabbits. These results also suggest that it may be possible to use the changes in various Hyp fractions in aortic tissues of rabbits as an important risk factor during the progression of atherosclerosis.

  15. COMPARATIVE ANTIHYPERLIPIDAEMIC EFFICACY OF TRACHYSPERMUM AMMI EXTRACTS IN ALBINO RABBITS

    Directory of Open Access Journals (Sweden)

    I. JAVED, Z. IQBAL, Z. U. RAHMAN, F. H. KHAN, F. MUHAMMAD, B. ASLAM AND L. ALI1

    2006-01-01

    Full Text Available Comparative antihyperlipidaemic efficacy of Trachyspermum ammi (L Sprague (Ajowain extracts in chloroform, methanol, petroleum ether and water was investigated in albino rabbits. Hyperlipidaemia was induced with butter fed ad libitum and oral intubation of cholesterol 400 mg/kg body weight. Simvastatin was used as a synthetic cholesterol lowering drug. The results suggested that chloroform and water extracts of T. ammi seed had no hypolipidaemic activity. However, methanol and petroleum ether extracts equivalent to its 2 g/kg body weight powder and Simvastatin (0.6 mg/kg body weight were equally effective in treating hyperlipidaemia in albino rabbits. Moreover, petroleum ether extract appeared to be more potent than methanol extract on the basis of increasing the level of HDL-cholesterol and lowering the LDL-cholesterol more effectively than methanol extract. Petroleum ether extract reduced atherogenic index (total cholesterol/HDL-cholesterol more effectively than methanol extract.

  16. The Year of the Rabbit

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Every year of the Chinese lunar calendar corresponds with an animal. The rat,ox, tiger, rabbit, dragon, snake, horse,sheep, monkey, rooster, dog and pig make up the Chinese zodiac, which repeats in a12-year cycle. This year’s Chinese NewYear rings in the Year of the Rabbit.

  17. Welfare assessment in pet rabbits

    NARCIS (Netherlands)

    Schepers, F.; Koene, P.; Beerda, B.

    2009-01-01

    One million pet rabbits are kept in The Netherlands, but there are no data available on their behaviour and welfare. This study seeks to assess the welfare of pet rabbits in Dutch households and is a first step in the development of a welfare assessment system. In an internet survey, housing

  18. INFECTIOUS MYXOMATOSIS OF RABBITS

    Science.gov (United States)

    Rivers, T. M.

    1930-01-01

    The virus of infectious myxomatosis of rabbits (Sanarelli) induces multiple lesions in the skin, lymph glands, tunica vaginalis,epididymis, testicle, spleen, and lungs. Growth and destruction of cells in the epidermis overlying the myxomatous masses leads to the formation of vesicles. Cytoplasmic inclusions are found in affected epidermal cells. Occasionally, similar inclusions are seen in other involved epithelial cells. The nature of the inclusions is an open question. In the myxomatous masses situated in the subcutaneous and other tissues, evidences of alteration and growth of certain cells are observed. PMID:19869741

  19. The White Rabbit project

    CERN Document Server

    Serrano, J; Gousiou, E; van der Bij, E; Wlostowski, T; Daniluk, G; Lipinski, M

    2013-01-01

    White Rabbit (WR) is a multi-laboratory, multi- company collaboration for the development of a new Ethernet-based technology which ensures sub-nanosecond synchronisation and deterministic data transfer. The project uses an open source paradigm for the development of its hardware, gateware and software components. This article provides an introduction to the technical choices and an explanation of the basic principles underlying WR. It then describes some possible applications and the current status of the project. Finally, it provides insight on current developments and future plans.

  20. Aqueous extract of Piper sarmentosum decreases atherosclerotic lesions in high cholesterolemic experimental rabbits

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    Raj Santhana

    2010-04-01

    Full Text Available Abstract Background Piper sarmentosum (P.s has flavonoid component in its leaves which has antioxidative effect. To date, its effect on atherosclerosis has not been studied histologically. Aim The study aimed to investigate the effect of P.s on atherosclerotic changes in hypercholesterolemic rabbits. Methods Forty two male New Zealand white rabbits were divided into seven groups. C - control group fed normal rabbit chow, CH - cholesterol diet (1% cholesterol, W1 - 1% cholesterol with water extract of P.s (62.5 mg/kg, W2 - 1% cholesterol with water extract of P.s (125 mg/kg, W3 - 1% cholesterol with water extract of P.s (250 mg/kg, W4 - 1% cholesterol with water extract of P.s (500 mg/kg and Smv - 1% cholesterol supplemented with simvistatin drug (1.2 mg/kg. All rabbits were treated for 10 weeks. Following 10 weeks of supplementation, the animals were sacrificed and the aortic tissue was taken for histological study. Results Rabbits fed only with high cholesterol diet 1% cholesterol (CH showed focal fatty streak lesions compared to the C group and 1% cholesterol supplemented with simvistatin drug (Smv group. Atherosclerotic lesions in the 1% cholesterol group supplemented with P.s (500 mg/kg i.e. W4 group showed significant reduction (30 ± 6.0%, p Conclusion Administration of P.s extract has protective effect against atheroscleros

  1. Oculoscopy in Rabbits and Rodents.

    Science.gov (United States)

    Jekl, Vladimir; Hauptman, Karel; Knotek, Zdenek

    2015-09-01

    Ophthalmic diseases are common in rabbits and rodents. Fast and definitive diagnosis is imperative for successful treatment of ocular diseases. Ophthalmic examination in rabbits and rodents can be challenging. Oculoscopy offers great magnification for the examination of the ocular structures in such animals, including the evaluation of cornea, anterior eye chamber, limbus, iris, lens, and retina. To date, oculoscopy has been described only sporadically and/or under experimental conditions. This article describes the oculoscopy technique, normal and abnormal ocular findings, and the most common eye disorders diagnosed with the aid of endoscopy in rabbits and rodents.

  2. Evaluation of milled pearl millet in the feeding of growing rabbits

    Directory of Open Access Journals (Sweden)

    Fernanda Catelan

    2012-08-01

    Full Text Available Two experiments were carried out in order to estimate the nutritional value and the performance of growing rabbits fed different levels of pearl millet (ADR 7010. In the digestibility trial, nutritional values of pearl millet were determined in 22 45-day-old New Zealand White rabbits, allotted in a completely randomized design, subjected to two treatments - a reference diet and a test diet with 70% of its volume composed of reference diet and 30% of pearl millet - and 11 replications. The apparent digestibility values of dry matter, crude protein, neutral detergent fiber and gross energy of the pearl millet were 88.7, 85.4, 24.4 and 75.0%, respectively. In the performance trial, 120 32-day-old New Zealand White rabbits were used. Rabbits were allotted in a completely randomized design and subjected to six treatments, with 10 replications and two animals for each experimental unit. The diets were formulated with increasing levels of pearl millet (20, 40, 60, 80 and 100%, substituting the corn in the reference diet, according to the digestible energy. No differences were observed in daily feed intake, daily weight gain, feed conversion, carcass traits and feed cost per kilogram of rabbit. Regardless of the amount of corn substituted, pearl can replace corn in diets for growing rabbits.

  3. Effect of aguamiel (agave sap on hematic biometry in rabbits and its antioxidant activity determination

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    Héctor Silos-Espino

    2011-04-01

    Full Text Available In this work, we analysed the effect of aguamiel (sap from Agave salmiana on haematic biometry in rabbits as well as its antioxidant activity with the objective of determining its nutritional value. Nine male Landrace 55-day-old rabbits were sorted into three groups of three rabbits each, which had access to fresh aguamiel, boiled aguamiel and water instead of aguamiel. Commercial rabbit food was supplied ad libitum to each experimental unit. Fresh aguamiel promoted weight increases of about 13% by the seventh week. Haemoglobin counts increased by 4.5% and 9% when rabbits were fed with fresh and boiled aguamiel, respectively. Haematocrit counts increased from 2.6% to 5.3%. Mean corpuscular volume did not show a change. Mean corpuscular haemoglobin significantly increased up to 12% above that previously reported on rabbits. Observed Fe (fixed to transferrin, transferrin and ferritin content increased slightly when fresh aguamiel was included in the diet, as compared to the control. In bone, Fe increased 79.3% (boiled aguamiel while P increased 23.6% (fresh aguamiel. In antioxidant activity determination, the aguamiel showed 20% with respect to that found in coffee and/or grape juice (commercial beverages with a high antioxidant capability, 226.2 µmol gallic acid equivalent and 35.1 mg ascorbic acid in 250 mL, respectively. These results showed that aguamiel is an innocuous beverage to feed animals, conferring some beneficial properties, and it could be considered as a nutraceutical product.

  4. Real-Time Elastography Visualization and Histopathological Characterization of Rabbit Atherosclerotic Carotid Arteries.

    Science.gov (United States)

    Wang, ZhenZhen; Liu, NaNa; Zhang, LiFeng; Li, XiaoYing; Han, XueSong; Peng, YanQing; Dang, MeiZheng; Sun, LiTao; Tian, JiaWei

    2016-01-01

    To evaluate the feasibility of non-invasive vascular real-time elastography imaging (RTE) in visualizing the composition of rabbit carotid atherosclerotic plaque as determined by histopathology, a rabbit model of accelerated carotid atherosclerosis was used. Thirty rabbits were randomly divided into two groups of 15 rabbits each. The first group was fed a cholesterol-rich diet and received balloon-induced injury the left common carotid artery endothelium, whereas the second group only received a cholesterol-rich diet. The rabbits were all examined in vivo with HITACHI non-invasive vascular real-time elastography (Hi-RTE) at baseline and 12 wk, and results from the elastography were compared with American Heart Association histologic classifications. Hi-RTE and the American Heart Association histologic classifications had good agreement, with weighted Cohen's kappa (95% confidence internal) of 0.785 (0.649-0.920). Strains of segmented plaques that were stained in different colors were statistically different (p visualizing normal and atherosclerotic rabbit carotid arteries non-invasively. This affordable and reliable method can be widely applied in research of both animal and human peripheral artery atherosclerosis.

  5. The Fed's Year of Transition

    Science.gov (United States)

    Schug, Mark C.; Niederjohn, Scott

    2006-01-01

    The purpose of this article is to: (1) Examine the historical development of the Federal Reserve System; (2) Provide background on Ben Bernanke, the new Fed chairman; (3) Explain the basic tools of monetary policy used by the Fed; (4) Examine the causes of the Great Depression, a topic of special interest to Bernanke; and (5) Provide some key…

  6. Effect of Humulus scandens for Traditional Fiber Sources on Digestion,Diarrhea,and Performance of Growing Rabbits

    Institute of Scientific and Technical Information of China (English)

    GUO Wan-hua; GU Zi-lin; WEI Kun-peng; LIU Ya-juan; WANG Zeng-li

    2009-01-01

    This work was to determine which concentration of a mixture of fresh Humulus scandens(HS)and fresh alfalfa could be included in diets for rabbits.The fresh forage of three experimental diets is composed of 100% alfalfa,50% alfalfa+50% HS,100% HS,respectively.30 rabbits[(1.42±0.056)kg]were used to determine nutrient digestibility.A feeding trial was carried out using 90 weaning rabbits[35 d of age;(512±6.5)g].Increasing content of HS in the diet did not affect digestibility of DM(dry matter),CF(crude fiber),CP(crude protein),GE(gross energy),and EE(ether extract).But diarrhea decreased markedly(P<0.01)compared with the diets that had no HS.ADFI(average daily feed intake)increased by 4.3%(P<0.05)and 3.6%(P<0.05)when rabbits fed the diet with 100% HS of fresh forage compared with the diets containing 0 and 50% HS,respectively.Feed efficiency improved significantly(P<0.05)by 2.07 and 5.30% when rabbits fed 0 and 50% HS of fresh forage,respectively,compared with 100% HS.There was no effect on ADG(average daily weight gain).In conclusion,the mixture(50∶50)of HS and alfalfa constitutes an alternative source of fiber for fattening rabbits and prevents diarrhea effectively.

  7. The cottontail rabbits of Virginia

    Science.gov (United States)

    Llewellyn, L.M.; Handley, C.O.

    1945-01-01

    Five races of cottontail rabbits belonging to three species occur in Virginia. One of them, the Mearns cottontail (Sylvilagus floridanus mearnsi), is reported here for the first time. It occurs in six southwestern counties of the state, while the eastern cottontail (S. f. mallurus) occurs in the remainder of the state with the exception of Smith and Fishermans islands off the eastern coast of Cape Charles, where it is replaced by Hitchens cottontail (S. f. hitchensi). The New England cottontail (S. transitionalis) is found on the higher mountain peaks, above 3000 feet, and the swamp rabbit (S. palustris) occurs in the Dismal Swamp region of southeastern Virginia.....The height of the breeding season for the eastern cottontail in Virginia is March and April, but breeding continues through the entire year except in December and January. The average litter size based on embryo counts was 4.7. The sex ratio of 234 specimens from all parts of the state, taken mostly in the December to February period, was 53 males to 47 females. That of a group of 145 rabbits live-trapped at Blacksburg during February and Marchwas 58 males to 42 females. The figures show that males are more active than females during the winter months, and therefore are more easily taken then....In transplanting cottontails from one section of the state to another, it is recommended that only cottontails of the same race as those originally present in the region being restocked be released there....Tularemia is not a common disease among rabbits in Virginia, but the rabbit ticks are often carriers of the disease and may transmit it to rabbits. Rabbit ticks are also found to be carriers of Rocky Mountain fever and American Q. fever. After the ticks drop off the rabbits to hibernate in the ground, which is likely to occur during mid-winter in Virginia, there is relatively little danger of humans contracting tularemia by contact with rabbits. Present laws in Virginia which prohibit rabbit hunting until the

  8. Effects of Feeding Periods of High Cholesterol and Saturated Fat Diet on Blood Biochemistry and Hydroxyproline Fractions in Rabbits

    Directory of Open Access Journals (Sweden)

    N. J. Siddiqi

    2008-01-01

    Full Text Available Hypercholesterolemia and hypertriglyceridemia are considered as important risk factors during the atherosclerotic process. The aim of the present investigation was to study the total cholesterol (TC, low-density lipoprotein cholesterol (LDLC, high density lipoprotein (HDL, triglyceride (TG, platelet levels and hydroxyproline fractions during the pathogenesis of atherosclerosis. For this purpose, twenty five 12-weeks, New Zealand white male rabbits, were purchased, individually caged, and divided into either control group or cholesterol-fed group. The control group (n = 10 was fed 100 g/day of normal diet, ORC-4 (Oriental Yeast Co. Ltd., Tokyo, Japan for a period of 15 weeks. The cholesterol-fed group (n = 15 was fed a high cholesterol and saturated fat diet of ORC-4 containing 1% cholesterol plus 1% olive oil (100 g/day for periods of 5 (group 1, 10 (group 2 and 15 (group 3 weeks. Blood sample from each animal was taken at the end of the experimental period for the biochemical analysis. The results of the present study showed that TC, LDLC, TG, HDLC and platelets were significantly (P0.05. There was no significant (P>0.05 decrease of free serum hydroxyproline in group 3 rabbits when compared to control rabbits. On the other hand, group 3 rabbits showed a significant increase in peptide–bound and protein- bound Hyp by 517% (P0.05 change when compared to control rabbits. These results suggest that feeding rabbits high cholesterol and saturated fat diet for feeding periods of 5 , 10 and 15 weeks induced significant change in TC, LDLC, HDL, TG, platelet levels and various Hyp fractions in serum without any significant change in the total Hyp content.

  9. Embryotoxicity/teratogenicity of isomalt in rats and rabbits.

    Science.gov (United States)

    Waalkens-Berendsen, D H; Koëter, H B; van Marwijk, M W

    1990-01-01

    The embryotoxicity/teratogenicity of the sugar replacer isomalt was studied in Wistar rats and New Zealand White rabbits. Groups of 22-23 female rats were given diets containing isomalt at concentrations of 2.5, 5 or 10%, from day 0 to day 21 of pregnancy. The possible adverse effects of restricted feeding were studied in an additional group (food intake less than 80% of the control values). Groups of 36-37 female rabbits were given diets containing isomalt at concentrations of 2.5, 5 or 10%, from day 0 to day 29 of pregnancy. The female rats and rabbits were killed on days 21 and 29 of pregnancy, respectively. In both species no maternal toxicity occurred and no effects on reproductive performance nor on embryonic or foetal development were seen in any of the groups fed isomalt. The feeding of restricted amounts of stock diet to pregnant rats resulted in decreased maternal weight gain and lower uterus weights. Furthermore, this group had an increased number of resorptions and small foetuses, decreased foetus and placental weights and retarded bone development.

  10. YUCCA SCHIDIGERA AND ITS EFFECT ON RABBIT REPRODUCTION

    Directory of Open Access Journals (Sweden)

    Martina Földešiová

    2013-12-01

    Full Text Available The aim of this study was to evaluate the effect of Yucca schidigera plant on rabbit reproduction parameters. Six weeks old New Zealand White rabbit females (n=36 and males (n=20 were used in the experiment. Animals in the control groups (C does; n=12 and C bucks; n=7 were fed with commercially available diet and the diet in the experimental groups was enriched with 5g/100kg (E1 does; n=12 and E1 bucks; n=7 or 20 g/100kg (E2 does; n=12 and E2 bucks; n=6 of Yucca schidigera powder extract. The rabbits were fed for 280 days and weighed weekly. The highest semen concentration was in the second experimental group E2 (3.80±0.57 x 109/ml compared to control group C (2.84±0.33 x 109/ml and experimental group E1 (1.9±0.35 x 109/ml. Semen motility (E2- 85.55±6.19 %; E1- 85.28±2.27% and progressive motility (E2; 73.45±9.63 %; E1; 71.16±3.43% were higher in the experimental groups compared to the control groups (motility -78.69±5.17 %; progressive motility - 61.34±6.69 %. The highest conception and kindling rate was found out in the control group C (83 % and 83 %, respectively, slightly lower values were reported in the both experimental groups E1 and E2 (for conception rate - 63% and 63%, respectively; and for kindling rate - 63% and 63%, respectively. Evaluation of the number of liveborn young rabbits showed, in the experimental group 2 the number of liveborn young rabbits (9.43±1.65 was higher than in the control (C; 8.0±0.97 and the experimental group 1 (6.0±1.

  11. Effects of Oxidized Tallow on the Rabbit Serum Lipids and Antioxidant Activity of the In-vitro Lipids.

    Science.gov (United States)

    Zeb, Alam; Rahman, Waheed Ur

    2012-09-01

    This paper describes the effects of thermally oxidized tallow on the serum lipids profile and radical scavenging activity (RSA) of the lipids extracted from the different tissues of the rabbits. Tallow was thermally oxidized at 130℃ for 9, 18, 27, 36 and 45 h respectively. Thermally oxidized tallow was fed to the local strain of Himalayan rabbits for one week. Results show that oxidation increases the formation of hydroperoxides and decrease the level of radical scavenging activity of the tallow. The rabbit serum lipids profile showed a dose dependent increase in triglyceride, total cholesterol and LDL-cholesterol. However, no statistically significant increase was observed in the HDL-cholesterol with an increase of oxidation time. Serum glucose and rabbits body weight decrease significantly (p tallow is harmful and therefore an alternative way of cooking should be used.

  12. Effects of high fat-, cholesterol-enriched diet on the antioxidant defence mechanisms in the rabbit heart.

    Science.gov (United States)

    Lapenna, D; Del Boccio, G; Porreca, E; Pennelli, A; Mezzetti, A; De Gioia, S; Marzio, L; Di Ilio, C; Cuccurullo, F

    1992-01-01

    In 7 rabbits fed on hyperlipidic diet (0.5% cholesterol, 5% peanut oil and 5% lard) for 4 weeks, the ventricular myocardium was tested for antioxidant defences and thiobarbituric acid reactive substances. Seven age-matched rabbits served as controls. The hearts were previously subjected to 45 min Langendorff perfusion to study coronary flow, developed tension and resting tension; coronary effluent values of CPK activity, pH and UV absorbance at 250 nm (i.e., low molecular weight ATP catabolites) were also investigated. After 4 weeks of diet, a significant rise of plasma cholesterol (P effluent of hyperlipidemic rabbits. In conclusion, high fat-, cholesterol-enriched diet induces an imbalance in the rabbit heart antioxidant defences, some of which are increased, whereas others are depressed, eventually resulting in enhanced myocardial lipid peroxidation. These biochemical changes are associated with higher perfusate values of UV absorbance at 250 nm, but not with significant CPK leakage or myocardial hemodynamics derangement.

  13. Resveratrol Protects Rabbits Against Cholesterol Diet-Induced Hyperlipidaemia.

    Science.gov (United States)

    Tanko, Y; Jimoh, A; Ahmed, A; Mohammed, A; Ayo, J O

    2016-08-30

    The excessive consumption of high cholesterol diet has been associated with an increased incidence oflipidaemia. Lipidaemia is enhanced by formation of oxidative stress, lipid peroxidation and hyperglycaemia. The aim ofthese experiments was to investigate the protective effect of resveratrol co-administered with cholesterol diet inducedhyperlipidaemia in rabbits. Thirty rabbits divided into six groups of five animal (group= 5) each: group 1 = normal control,group 2 = cholesterol diet/high fat diet group only (HFD), group 3 = resveratrol 200 mg/kg (R200), group 4 = resveratrol400 mg/kg (R400), group 5 = HFD + R200 and group 6 = HFD + R400. The normal group was fed with standard animalfeeds only; while the HFD groups were fed with standard animal feeds + cholesterol diet (10% Groundnut oil, 20%Groundnut mill and 2% cholesterol). Resveratrol-treated rabbits received resveratrol suspended in 10 g/Lcarboxymethylcellulose (CMC) and the control group received the vehicle only, CMC. The preparations were administeredfor 8 weeks of experimental protocol. At the end of the study period, the animals were sacrificed. Blood and plasma sampleswere collected. Serum evaluation of lipid profile such as total cholesterol (TC), triacylglycerol (Tg), low density lipoproteincholesterol (LDP-c) and high density lipoprotein cholesterol (HDL-c) were also assessed. The results obtained showsignificant (P < 0.05) decrease in total cholesterol (TC), Low density lipoprotein cholesterol (LDP-c), total triacylglyceroland an increase in high density lipoprotein cholesterol (HDL-c) in resveratrol treated groups compared to HFD group only.In conclusion, the findings indicated that Resveratrol may contain polar products able to lower plasma lipid concentrationsand might be beneficial in treatment of hyperlipidemia and atherosclerosis.

  14. The White Rabbit Project

    CERN Document Server

    Serrano, J; Cattin, M; Garcia Cota, E; Lewis, J; Moreira, P; Wlostowski, T; Gaderer, G; Loschmidt, P; Dedic, J; Bär, R; Fleck, T; Kreider, M; Prados, C; Rauch, S

    2009-01-01

    Reliable, fast and deterministic transmission of control information in a network is a need formany distributed systems. One example is timing systems, where a reference frequency is used to accurately schedule time-critical messages. TheWhite Rabbit (WR) project is a multi-laboratory and multi-company effort to bring together the best of the data transfer and timing worlds in a completely open design. It takes advantage of the latest developments for improving timing over Ethernet, such as IEEE 1588 (Precision Time Protocol) and Synchronous Ethernet. The presented approach aims for a general purpose, fieldbus-like transmission system, which provides deterministic data and timing (sub-ns accuracy and ps jitter) to around 1000 stations. It automatically compensates for fiber lengths in the order of 10 km. This paper describes the WR design goals and the specification used for the project. It goes on to describe the central component of the WR system structure - the WR switch - with theoretical considerations a...

  15. Semen characteristics and sexual hormones of Hy-Plus rabbit bucks influenced by a dietary multi-enzymes additive

    Directory of Open Access Journals (Sweden)

    H. Gado

    2015-06-01

    Full Text Available A total of 144 adult Hy-Plus rabbit bucks were randomly assigned into  four treatments of 36 replicates each, in a completely randomized design. Animals were fed ad libitum on basal diet supplemented with an enzyme complex (EZ at 0 (EZ0, 1 (EZ1, 3 (EZ3 and 5 (EZ5 kg/ton of feed. Total sperm count was higher (P<0.05 in EZ5 than in EZ0. Sperm motility increased with increasing levels of the EZ additive being lowest for EZ0 and highest for EZ5 rabbits (P<0.05. Percentage of dead sperm was higher (P<0.05 in EZ0 than in EZ5 rabbits. Rabbit bucks received the highest levele of EZ in their diet, had the shorter reaction time (22.31±3.17 sec; P<0.05,whereas EZ5 bucks took 43.56±5.89 sec to mount does for the first time after exposure. Blood testosterone, 17α-estradiol and progesterone levels were highest in EZ5 rabbits and lowest in EZ0 rabbits. Enzyme addition increased (P<0.05 sperm transit in estrus doe cervical mucus. Data suggested that the EZ additive in diets of  adult Hy-Plus rabbit bucks was effective to improve both semen characteristics and sexual drive.

  16. Effect of Digestarom® dietary supplementation on the reproductive performances of rabbit does: preliminary results

    Directory of Open Access Journals (Sweden)

    Chiara Celia

    2015-12-01

    Full Text Available The study investigated the effect of Digestarom® dietary supplementation on the reproductive performances of rabbit does. Pannon Ka (maternal line multiparous does were randomly divided into two dietary groups since insemination and fed ad libitum. In the first group (n=51, rabbit does were fed with a commercial diet (C, whereas in the other group (n=52 they received the same diet supplemented with 300 mg/kg of Digestarom® (D. The experiment lasted for two reproductive cycles (kindling 1=K1; kindling 2=K2. Body weight of does and litter size (kits born total, alive, stillborn were recorded at kindling. Litter size and litter weight were registered at 7, 14, 21 days of age after nursing, and the average individual weight of kits was calculated. Kits’ mortality was recorded daily. At K1, rabbit does performances were unaffected by dietary treatments. During K2, D does were significantly lighter than C ones (P<0.05 and displayed a lower kindling rate (P<0.05. Digestarom® did not improve the reproductive performance of rabbit does. They seemed to dislike the D diet in K1 and such behavior could have led to the negative results in K2. Further studies should focus on feed acceptance, dose-dependent effect, physiological adaptation and in vivo oxidative status of does. Finally, several consecutive reproductive cycles are recommended to test the efficacy of new feed supplements.

  17. [Effects of cholesterol rich diet on blood coagulative and fibrinolytic activities in male rabbits].

    Science.gov (United States)

    Si, Quan-jin; Li, Xiao-ying

    2005-05-01

    To explore the effects of cholesterol rich diet on the activities of blood coagulative and fibrinolytic systems in male rabbits. 14 male New Zealand white rabbits were randomized to cholesterol rich diet(CRD) group and common diet (control) group. Rabbits in CRD group were fed with 1% cholesterol embedded diet and those in the control group were fed with common diet. Levels of blood TG, TC, LDL, HDL, Lp(a), apoA1, apoB, FIB, D-dimers and FDP, PT and APTT, activity of ADP, AT-III, PLG and alpha2-PI were tested in all rabbits before given cholesterol rich diet and after 12 weeks' feeding with different kinds of diet. Levels of blood TG, TC, LDL, HDL, Lp(a), apoA1, apoB, FIB, D-dimers in CRD group were all elevated significantly compared with those in the control group and the baseline levels. PT and APTT were shortened, ADP, PLG and alpha2-PI activity were increased in CRD group. Cholesterol rich diet not only is the direct cause of hyperlipidemia but also can increase the coagulative activity and inhibit the fibrinolytic activity and promoting the evolution of arteriosclerosis.

  18. Neuroprotective Effects of Low-Dose Statins in the Retinal Ultrastructure of Hypercholesterolemic Rabbits.

    Science.gov (United States)

    Fernández-Navarro, Judith; Aldea, Pilar; de Hoz, Rosa; Salazar, Juan J; Ramírez, Ana I; Rojas, Blanca; Gallego, Beatriz I; Triviño, Alberto; Tejerina, Teresa; Ramírez, José M

    2016-01-01

    To evaluate the pleiotropic effects to statins, we analyze the qualitative and quantitative retinal changes in hypercholesterolemic rabbits after a low-dosage statin treatment. For this purpose, New Zealand rabbits were split into three groups: control (G0; n = 10), fed a standard diet; hypercholesterolemic (G1; n = 8), fed a 0.5% cholesterol-enriched diet for 8 months; and statins (G2; n = 8), fed a 0.5% cholesterol-enriched diet for 8 months, together with the administration of statin (pravastatin or fluvastatin sodium) at a dose of 2 mg / kg / day each diet. The retinas were analyzed by transmission electron microscopy and immunohistochemistry (glial fibrillary acidic protein). The retinal thickness of nuclear and plexiform layers were quantified in semi-thin sections. The results revealed that the low-statin-treated rabbits in comparison with the hypercholesterolemic group showed: i) a more preserved structure in all retinal layers; ii) a significant reduction in retinal thickness; iii) a decrease in cell death in the nuclear-and ganglion-cell layers; iv) a reduction of hydropic degeneration in the plexiform and nerve-fiber layers; v) a preservation of astrocytes and of the retinal area occupied by them; and vi) a better-preserved retinal vascular structure. Our findings indicate that low doses of statins can prevent retinal degeneration, acting on retinal macroglia, neurons and retinal vessels, despite that hypercholesterolemia remained unchanged. Thus, the pleiotropic effects of the statins may help safeguard the retinal ultrastructure.

  19. Neuronal quantity and ultrastructure in extracorticospinal tract of newborn rabbits with cerebral palsy

    Institute of Scientific and Technical Information of China (English)

    Xiaojie Li; Ying Yang; Zhimei Jiang; Lanrnin Guo; Jing Gao

    2006-01-01

    BACKGROUND: The liver enzyme system and blood brain barrier function of newborn rabbits are incomplete.High level of bilirubin can lead to cerebral palsy (CP) of newborn rabbits. The pathological and ultrastructural changes of brains of immature rabbits may differ from those of mature rabbits.OBJECTIVE: To observe the changes in dopaminergic neuron amount and ultrastructure in the extracorticospinal tract of animal models of CP induced by hyperbilirubinemia.DESIGN: A randomized controlled observation.SETTING: Rehabilitation Medical College of Jiamusi University; Laboratory for Pediatric Neurology Rehabilitation of Jiamusi University.MATERIALS: Thirty newborn 2- to 5- day inbred Japanese rabbits, weighing about 50 g, of either gender, were provided by Experimental Animal Center, Jilin University. Bilirubin and tyrosine hydroxylase (TH) antibody were purchased from Sigma Corporation, USA.METHODS: This experiment was carried out in the laboratory for children neuro-rehabilitation, Jiamusi University between September 2002 and December 2005. ①Thirty rabbits were randomly divided into 2groups: control group (n =10) and model group (n =20). ②Bilirubin of 100 mg/kg was given to the rabbits of model group through intraperitoneal injection, once every other day, 3 times totally; The same amount of normal saline was given to the rabbits of control group. After injection, the newborn rabbits had been fed by maternal milk for 45 days. ③TH-positive neurons in substantia nigra and corpora striatum in each area of0.15 mm ×0.15 mm were counted. Uitrastructures of substantia nigra and corpora striatum were observed under the electron miscroscope. The concentration of bilirubin in brain tissue of CP rabbits of model group was measured with high performance liquid chromatogram.MAIN OUTCOME MEASURES: Quantitative analysis results and ultrastructure of TH-positive neurons in substantia nigra and corpora striatum in two groups.RESULTS: Thirty newborn rabbits were involved, 6

  20. Teratology studies in the rabbit.

    Science.gov (United States)

    Allais, Linda; Reynaud, Lucie

    2013-01-01

    The rabbit is generally the non-rodent species or second species after the rat recommended by the regulatory authorities and is part of the package of regulatory reproductive studies for the detection of potential embryotoxic and/or teratogenic effects of pharmaceuticals, chemicals, food additives, and other compounds, including vaccines (see Chapters 1-7).Its availability, practicality in housing and in mating as well as its large size makes the rabbit the preferred choice as a non-rodent species. The study protocols are essentially similar to those established for the rat (Chapter 9), with some particularities. The study designs are well defined in guidelines and are relatively standardized between testing laboratories across the world.As for the rat, large litter sizes and extensive background data in the rabbit are valuable criteria for an optimal assessment of in utero development of the embryo or fetus and for the detection of potential external or internal fetal malformations.

  1. [Review] Mary Toft's Rabbit Tale

    OpenAIRE

    Jones, Emrys

    2013-01-01

    Originally broadcast in April 2011 and aired again by BBC Radio 4 this November, Mary Toft’s Rabbit Tale is a radio drama retelling the story of its titular fraudster’s brief notoriety. With a high-profile cast – including singer Will Young as Toft’s husband and Rupert Graves as man-midwife, John Howard – the play explores a number of issues related to the alleged rabbit births of 1726 and their impact on public discourse of the time.

  2. Hypercholesterolemia increases plasma saturated and n-6 fatty acids altering prostaglandin homeostasis and promotes endothelial dysfunction in rabbits.

    Science.gov (United States)

    Medina, M; Alberto, M R; Sierra, L; Van Nieuwenhove, C; Saad, S; Isla, M I; Jerez, S

    2014-07-01

    The present study evaluated the plasma fatty acid levels and the vascular prostaglandin (PG) release in a rabbit model of early hypercholesterolemia with endothelial dysfunction. Rabbits were fed either a control diet (CD) or a diet containing 1 % cholesterol (HD) for 5-6 weeks. The level of fatty acids was measured in plasma. The levels of PG and nitric oxide (NO) released from the aorta were also determined. Vascular morphology of the aorta was characterized by intima and media thickness measurements. The rabbits fed with HD had higher levels of arachidonic acid (ARA) and lower levels of oleic acid. The linoleic acid level was unchanged. PGI(2) and NO were diminished and PGF(2α) levels, the PGI(2)/TXA(2) ratio and the intima/media ratio were increased in rabbits fed with HD. In conclusion, feeding HD for a short period increased ARA plasma levels and unbalanced release of vasodilator/vasoconstrictor PG redirected the pathway to vasoconstrictor metabolite release. These lipid metabolism alterations in addition to the reduced NO levels and the moderate changes in the vascular morphology contributed to the endothelial dysfunction in this animal model. Therefore, the present findings support the importance of early correction or prevention of high cholesterol levels to disrupt the endothelial dysfunction process that leads to cardiovascular disease.

  3. Nutritive value of brewers’ grain and maize silage for fattening rabbits

    Directory of Open Access Journals (Sweden)

    H. Guermah

    2016-09-01

    Full Text Available Specific knowledge of the nutritive value of raw materials is fundamental to formulate balanced diets for rabbits and allows greater use of by-products and non-conventional feedstuffs. This paper examines the feeding value of sun-dried brewers’ grain and maize silage (whole plant for fattening rabbits. Twenty-four individually caged 8-wk-old rabbits were used to determine the digestibility. Both wet products were sun-dried and ground before being incorporated into a basal diet. The inclusion level at the expense of all basal ingredients amounted to 30%. Basal diet and both experimental diets were fed ad libitum to 8 rabbits during the 4-d balance trial. The determined digestibility of protein, fat, crude fibre and neutral detergent fibre digestibility amounted to 76.2 and 77.2%; 86.5 and 99.1%; 8.1 and 8.3% and 28.0 and 13.5%, respectively, for brewers’ grain and maize silage. The digestible energy content amounted to 11.66 MJ/kg dry matter (DM (brewers’ grain and 11.10 MJ/kg DM (maize silage. Both by-products have potential as alternative feedstuff in rabbit diets. However, further experiments are necessary to determine the effect of ensilaging the whole maize plant, as a significantly lower (P<0.001 feed intake was observed.

  4. Effect of Alchornea cordifolia leaf meal inclusion and enzyme supplementation on performance and digestibility of rabbits

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    S.O. Ayodele

    2016-09-01

    Full Text Available A feeding trial was conducted to study the performance, digestibility and health status of weaner rabbits fed diets including Alchornea cordifolia leaf meal (ALM: 18% crude protein [CP] and 12.9% crude fibre and supplemented with a multi-enzyme additive (cellulase, xylanase, β-glucanase, α-amylase, protease, lipase. Six experimental diets were arranged factorially: 3 levels of ALM (0, 5 and 10% substituting palm kernel cake: 16.3% CP and 39.1% neutral detergent fibre combined with 2 levels of enzyme supplementation (0 and 0.35 g/kg. One hundred and eighty healthy, 5-wk-old weaner rabbits of cross-breeds were randomly allotted to 6 dietary treatments (30 rabbits/treatment, 3 rabbits/replicate. Growth rate was not affected (P>0.05 by the main factors (exogenous enzyme and ALM inclusion and their interactions (13.5 g/d on av.. Daily feed intake and feed conversion ratio decreased (P=0.01 with the ALM inclusion by 8%, but did not affect faecal digestibility. However, enzyme supplementation improved crude protein and crude fibre digestibility (P<0.001 by 6%. In conclusion, ALM inclusion and enzyme supplementation had no adverse effect on the performance and digestibility of rabbits.

  5. Effects of rosiglitazone on contralateral iliac artery after vascular injury in hypercholesterolemic rabbits

    Directory of Open Access Journals (Sweden)

    Baroncini Liz

    2008-05-01

    Full Text Available Abstract Background The objective was to evaluate the effects of rosiglitazone on iliac arteries of hypercholesterolemic rabbits undergoing balloon catheter injury in the contralateral iliac arteries. Methods White male rabbits were fed a hypercholesterolemic diet for 6 weeks and divided into two groups as follows: rosiglitazone group, 14 rabbits treated with rosiglitazone (3 mg/Kg body weight/day during 6 weeks; and control group, 18 rabbits without rosiglitazone treatment. All animals underwent balloon catheter injury of the right iliac artery on the fourteenth day of the experiment. Results There was no significant difference in intima/media layer area ratio between the control group and the rosiglitazone group. Rosiglitazone did not reduce the probability of lesions types I, II, or III (72.73% vs. 92.31%; p = 0.30 and types IV or V (27.27% vs. 7.69%; p = 0.30. There were no differences in the extent of collagen type I and III deposition or in the percentage of animals with macrophages in the intima layer. The percentage of rabbits with smooth muscle cells in the intima layer was higher in rosiglitazone group (p = 0.011. Conclusion These findings demonstrate that rosiglitazone given for 6 weeks did not prevent atherogenesis at a vessel distant from the injury site.

  6. Effect of the dietary electrolyte balance on the reproductive response of rabbit does

    Directory of Open Access Journals (Sweden)

    C. Rizzi

    2011-03-01

    Full Text Available Mineral requirements of rabbits have been recently reviewed (Mateos and de Blas, 1998, but there is limited information on some macroelements of the diet. Furthermore there is a lack of indications on the effect of the dietary electrolyte balance, dEB (dEB= Na+K-Cl, on the reproductive response of does and bucks and there are few works on the zootechnical performance of growing rabbits (Harris et al., 1984; Bonsembiante et al., 1989. Currently, only Ca, P and Na are taken into account in practical formulation of rabbit diets. Current estimates indicate a range of 6.5-10.0, 2.0-2.5 and 2.8-4.8 g/kg, respectively for K, Na and Cl. Rabbit presents differences from other mammals fed the same type of diet because it produces more alkaline urine. So the rabbit is particularly vulnerable to acid loads and therefore it is important to avoid imbalances among these three minerals that might induce problems to kidneys and reproductive response or decreasing of feed intake...

  7. Local administration of growth hormone enhances periimplant bone reaction in an osteoporotic rabbit model.

    Science.gov (United States)

    Tresguerres, Isabel F; Clemente, Celia; Donado, Manuel; Gómez-Pellico, Luis; Blanco, Luis; Alobera, Miguel Angel; Tresguerres, Jesús A F

    2002-12-01

    The purpose of this study was to determine whether rhGH administered locally during the surgical placement of an implant, in the tibia of an osteoporotic rabbit model, would induce qualitative and quantitative differences in peri-implant bone reaction. Eight New Zealand rabbits were ovariectomized and fed with a low-calcium diet (with 0.07% of calcium) to induce osteoporosis. After 6 weeks, an experimental titanium sheet was inserted into the rabbit tibiae. The rabbits were randomly divided into two groups. The experimental group was treated with 4 IU of rhGH added locally into the ostectomy as a lyophilized powder and the control group was left without any treatment. After 2 weeks, animals were sacrificed, tibial sections were prepared and bone-titanium interface was examined at light microscopy, using Masson, hematoxylin-eosin and Pichrosirius stains. Light microscopic morphometry and densitometry were used to comparatively quantitate bone reaction. Local administration of rhGH during the surgical placement of titanium sheets on the tibiae of an osteoporotic rabbit model enhances periosteal and transcortical reaction and mineralization of osteoid 14 days later around titanium sheets, without increasing bone resorption.

  8. Effect of chocolate and Propolfenol on rabbit spermatogenesis and sperm quality following bacterial lipopolysaccharide treatment.

    Science.gov (United States)

    Collodel, Giulia; Moretti, Elena; Del Vecchio, Maria Teresa; Biagi, Marco; Cardinali, Raffaella; Mazzi, Lucia; Brecchia, Gabriele; Maranesi, Margherita; Manca, Daniela; Castellini, Cesare

    2014-08-01

    The aims of the study were to evaluate the effects of chocolate and propolis-enriched diets on rabbit spermatogenesis, sperm motility, and ultrastructure following bacterial lipopolysaccharide (LPS) treatment. Thirty-two New Zealand White rabbits were divided into four groups. The LPS-Propolfenol(®) group received propolis (500 mg/kg/day) in their diet for 15 days, while the LPS-chocolate group was fed 70% cacao chocolate (1 g/1 kg/day) for the same period. Following the diet treatments, rabbits in the LPS-Propolfenol(®) and LPS-chocolate groups, and an LPS group received a single intraperitoneal dose of 50 μg/kg LPS, and the control group received only saline. Kinematic sperm traits were evaluated with a computer assisted sperm analyzer (CASA) system, and ultrastructural characteristics were examined by transmission electron microscopy (TEM). Testicular and epididymal tissues were observed by light microscopy and TEM and multiplex real time reverse transcriptase-polymerase chain reaction (RT-PCR) assay was used to detect and quantify toll-like receptor-4 (TLR-4) gene expression. The values of the analyzed semen parameters of rabbits treated with LPS-Propolfenol(®) and LPS-chocolate did not show any variations compared with the control group, but they were lower in rabbits treated only with LPS. Alterations observed in the testicular tissue of LPS treated-rabbits were not detected in specimens from the LPS-chocolate and LPS-Propolfenol(®) groups, which showed normal spermatogenesis. The TLR-4 mRNA expression was similar in controls, in LPS treated, and in LPS-chocolate groups, but it was significantly (p chocolate and propolis-enriched diet showed a protective effect on the spermatogenetic process of buck rabbits following LPS treatment.

  9. The effect of 1-week feed restriction on performance, digestibility of nutrients and digestive system development in the growing rabbit.

    Science.gov (United States)

    Tůmová, E; Volek, Z; Chodová, D; Härtlová, H; Makovický, P; Svobodová, J; Ebeid, T A; Uhlířová, L

    2016-01-01

    A 3 to 4 week feed restriction of about 20% to 25% of the free intake is widely applied in rabbit breeding systems to reduce post-weaning digestive disorders. However, a short intensive feed restriction is described in few studies and can be beneficial for growing rabbits due to a longer re-alimentation period. The aim of this study was to evaluate the effect of ad libitum (AL) and two restriction levels of feeding (50 and 65 g/rabbit per day) applied for 1 week on performance, gastrointestinal morphology and physiological parameters during the restriction and during the re-alimentation period. Rabbits were divided into three experimental groups: AL rabbits were fed AL, R1 rabbits were restricted from 42 to 49 days of age and received 50 g daily (29% of AL) and R2 rabbits were restricted at the same age and were fed 65 g of feed daily (37% of AL). In the 1(st) week after weaning and in the weeks after restriction, all the groups were fed AL. During the restriction period, daily weight gain (DWG) in R1 significantly dropped to 11% (experiment 1) and 5% (experiment 2) compared with rabbits in the AL group, although they were fed 29% of AL, whereas in the R2 group it decreased to 20% (experiment 1) and 10% (experiment 2). In the week following feed restriction, DWG in the restricted groups increased (Prabbits in both the experiments reached up to 90% to 93% of the final live weight (70 days) of the AL group. Over the entire experimental period, feed restriction significantly decreased feed intake to 85% to 88% of the AL group; however, the feed conversion ratio was lower (P<0.05) only in experiment 1 (-6% in R1 and -4% in R2). Digestibilities of CP and fat were not significantly higher during the restriction period and during the 1(st) week of re-alimentation compared with the AL group. Significant interactions between feeding regime and age revealed the shortest large intestine in the AL group at 49 days of age and the longest at 70 days in the AL and R1 groups

  10. Nature's food anticipatory experiment: entrainment of locomotor behavior, suprachiasmatic and dorsomedial hypothalamic nuclei by suckling in rabbit pups.

    Science.gov (United States)

    Caba, Mario; Tovar, Anibal; Silver, Rae; Mogado, Elvira; Meza, Enrique; Zavaleta, Yael; Juárez, Claudia

    2008-01-01

    In nature and under laboratory conditions, dams nurse rabbit pups once daily for a duration of fewer than 5 min. The present study explored neural mechanisms mediating the timing of nursing in this natural model of food anticipatory activity, focussing on the suprachiasmatic nucleus (SCN), the locus of the master circadian clock and on the dorsomedial hypothalamic nucleus (DMH), a region implicated in timing of food-entrained behavior. Rabbit pups are born in the dark, with eyelids closed. Nursing visits to the litters also occurs during the dark phase. To explore the effect of the timing of feeding, pups were maintained in constant darkness, while females housed in a light-dark cycle were permitted to nurse their pups either during the night (night-fed group) or day (day-fed group). All pups exhibited anticipatory locomotor activity before daily nursing. In the SCN, PER1 and FOS peaked during the night in both groups, with a longer duration of elevated protein expression in the night-fed group. In contrast, DMH peak PER1 expression occurred 8 h after pups were fed, corresponding to the shift in timing of nursing. Comparison of nursed and 48 h fasted pups indicates that the timing of PER1 expression was similar in the SCN and DMH, with fewer PER1-positive cells in the latter group. The results indicate that rabbit pups show food anticipatory activity, and that timing of nursing differentially affects PER1 expression in the SCN and DMH.

  11. Microbiological quality of rabbit meat.

    Science.gov (United States)

    Rodríguez-Calleja, Jose M; Santos, Jesús A; Otero, Andrés; García-López, María-Luisa

    2004-05-01

    World rabbit meat production is estimated to be over 1 million tons, and Spain is the third largest producer. Although rabbit meat is marketed and consumed worldwide, information on microbiological quality is very scarce. Here, we report indicator organisms, spoilage flora, sensory quality, and some physicochemical traits of 24 h postmortem chilled rabbit carcasses and prepackaged rabbit meat stored chilled in air for 0 to 3 days at the retail level. The mean total bacterial count (4.01 +/- 0.48 log CFU/g) for carcasses dressed at a small abattoir by a manual process was significantly lower (P Pseudomonas, lactic acid bacteria, and yeasts. These microorganisms and Brochothrix thermosphacta were dominant on carcasses from the large abattoir. On prepacked hind legs (pH 6.26 +/- 0.18) stored at -1 to +1 degree C (supermarket 1), mean aerobic mesophilic count was 5.87 +/- 1.03 log CFU/g, and the major microbial groups were Pseudomonas, yeasts, lactic acid bacteria, and B. thermosphacta. On prepacked whole carcasses (pH 6.37 +/- 0.18) displayed at -1 to +5 degrees C (supermarket 2), mean aerobic mesophilic count was 6.60 +/- 1.18 and the same microbial groups were dominant. Relative Escherichia coli incidence was supermarket 2 > large abattoir > supermarket 1 > small abattoir. Overall, low numbers of coliforms, Enterobacteriaceae, psychrotrophic clostridia, coagulase-positive staphylococci, and molds were found. Sensory scores, pH values, and L-lactic acid content differentiated fresh carcasses from retail samples. Data obtained suggest that the microflora of chilled rabbit meat are different from those found on the meat of other animals.

  12. Effect of flaxseed on choroid-sclera complex thickness and on LDL oxidation in the sclera, choroid and retina of diet-induced hypercholesterolaemic rabbits.

    Science.gov (United States)

    Torres, Rogil José de Almeida; Luchini, Andrea; Both, Alessandro Soares; Precoma, Leonardo Brandao; Champoski, Ana Flavia; Schuh, Maynara Leonardi; Torres, Renan Pedro de Almeida; Noronha, Lucia; Pessoa, Bernardo Molinari; Torres, Lucas Antonio de Almeida; Serci, Nicole Francesca de França; Precoma, Dalton Bertolim

    2014-11-14

    The aim of the present study was to evaluate the effect of flaxseed on choroid-sclera complex thickness and on LDL oxidation in the sclera, choroid and retina of diet-induced hypercholesterolaemic rabbits. New Zealand male albino rabbits (n 21) were divided into two groups: group 1 (G1; n 11), fed a hypercholesterolaemic diet, and group 2 (G2; n 10), fed a hypercholesterolaemic diet enriched with flaxseed flour. The serum concentrations of total cholesterol (TC), LDL-cholesterol (LDL-C), HDL-cholesterol, TAG and fasting blood glucose were determined at the start of the experiment and on the day of killing (8th week). Choroid and sclera samples were subjected to haematoxylin-eosin (HE) staining and histomorphometric and immunohistochemical analyses with the anti-oxidised LDL antibody. Sensory retina samples were subjected to an immunohistochemical analysis with the primary monoclonal nitrotyrosine antibody. At the end of the experiment, a significant increase was observed in TC and LDL-C concentrations in G1 rabbits when compared with G2 rabbits (P= 0·008 and P= 0·02, respectively). HE staining revealed a significant increase in choroid-sclera complex thickness in G1 rabbits when compared with G2 rabbits (Pchoroid and sclera samples with the anti-oxidised LDL marker revealed a significant increase in immunoreactivity in G1 rabbits when compared with G2 rabbits (Pchoroid-sclera complex thickness of diet-induced hypercholesterolaemic rabbits and the expression of oxidised LDL in the choroid-sclera complex as well as the expression of nitrotyrosine in the sensory retina.

  13. RPR test for serological survey of rabbit syphilis in companion rabbits.

    Science.gov (United States)

    Saito, Kumiko; Tagawa, Masayo; Hasegawa, Atsuhiko

    2003-07-01

    Since the RPR (rapid plasma regain) test was found to be useful for the diagnosis of rabbit syphilis, serological survey by this test has been carried out in Japanese companion rabbits. A hundred virgin household rabbits kept alone and without signs and history of syphilis were examined by RPR test from April 2001 to March 2002, in Tokyo, Japan. The test was positive in 35 cases and negative in 65 cases. RPR negative rabbits should be selected for breeding to prevent the spread of rabbit syphilis in companion rabbits in Japan.

  14. Microglial activation in the hippocampus of hypercholesterolemic rabbits occurs independent of increased amyloid production

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    Streit Wolfgang J

    2007-08-01

    Full Text Available Abstract Background Rabbits maintained on high-cholesterol diets are known to show increased immunoreactivity for amyloid beta protein in cortex and hippocampus, an effect that is amplified by presence of copper in the drinking water. Hypercholesterolemic rabbits also develop sporadic neuroinflammatory changes. The purpose of this study was to survey microglial activation in rabbits fed cholesterol in the presence or absence of copper or other metal ions, such as zinc and aluminum. Methods Vibratome sections of the rabbit hippocampus and overlying cerebral cortex were examined for microglial activation using histochemistry with isolectin B4 from Griffonia simplicifolia. Animals were scored as showing either focal or diffuse microglial activation with or without presence of rod cells. Results Approximately one quarter of all rabbits fed high-cholesterol diets showed evidence of microglial activation, which was always present in the hippocampus and not in the cortex. Microglial activation was not correlated spatially with increased amyloid immunoreactivity or with neurodegenerative changes and was most pronounced in hypercholesterolemic animals whose drinking water had been supplemented with either copper or zinc. Controls maintained on normal chow were largely devoid of neuroinflammatory changes, but revealed minimal microglial activation in one case. Conclusion Because the increase in intraneuronal amyloid immunoreactivity that results from administration of cholesterol occurs in both cerebral cortex and hippocampus, we deduce that the microglial activation reported here, which is limited to the hippocampus, occurs independent of amyloid accumulation. Furthermore, since neuroinflammation occurred in the absence of detectable neurodegenerative changes, and was also not accompanied by increased astrogliosis, we conclude that microglial activation occurs because of metabolic or biochemical derangements that are influenced by dietary factors.

  15. Effect of dietary inclusion of Leucaena leucocephala or Moringa oleifera leaf meal on performance of growing rabbits.

    Science.gov (United States)

    Safwat, Assem Mohamed; Sarmiento-Franco, Luis; Santos-Ricalde, Ronald; Nieves, Duilio

    2014-10-01

    This experiment was carried out to evaluate the effect of including either Leucaena leucocephala (LLM) or Moringa oleifera leaf meal (MOLM) as tropical feed resources at two levels (30 or 40 %) on growth performance, carcass traits, and economical efficiency of growing rabbits. A total of 60 California growing rabbits were randomly distributed into five experimental groups, each consisting of 12 rabbits and were allocated individually; the groups were control, 30 % LLM, 40 % LLM, 30 % MOLM, and 40 % MOLM. The experimental period lasted from 6 to 12 weeks of age. The results showed that rabbits fed control and 30 % MOLM diets had significantly the highest final body weight and daily weight gain being 2,040 and 2,000 g and 31.6 and 30.6 g/day, respectively. Similarly, the best feed conversion ratio was associated with 30 % MOLM and control groups (3.2 and 3.4), while the worst value was for 40 % LLM group (5.2). MOLM treatments recorded significantly the highest dressing percentage along with control group. The inclusion of MOLM by 30 and 40 % improved the economical efficiency (2 and 1.5) in comparison with the control group (1.1). The results suggest that MOLM can be included in growing rabbit diets up to 40 % without any adverse effect on growth performance or carcass traits with higher economical efficiency of growing rabbits.

  16. The effect of dietary Digestarom® herbal supplementation on rabbit meat fatty acid profile, lipid oxidation and antioxidant content.

    Science.gov (United States)

    Mattioli, S; Dal Bosco, A; Szendrő, Zs; Cullere, M; Gerencsér, Zs; Matics, Zs; Castellini, C; Dalle Zotte, A

    2016-11-01

    The experiment tested the effect of Digestarom® herbal supplementation on the antioxidant content, lipid oxidation and fatty acid profile of rabbit meat. At kindling, rabbit does and litters were divided into two dietary groups (N=162 kits/dietary group) and fed either a control diet (C) or the C diet supplemented with Digestarom® (D: 300mg/kg). At weaning (35days) four experimental fattening groups (54 rabbits each) were considered: CC, CD, DC and DD. After slaughtering (12weeks of age), Longissimus thoracis et lumborum muscles were dissected from 20 rabbits/group and analyzed. Rabbit meat of DD group was enriched in essential C18:3 n-3 fatty acid and in other long-chain PUFA of n-3 series. Despite meat of DD group displayed the highest peroxidability index, TBARs value was the lowest. Meat antioxidant content followed the rank order: DD>CD>DC>CC. Digestarom® improved fatty acid composition and oxidative status of rabbit meat, particularly when administered from weaning throughout the growing period.

  17. Immunosuppression abrogates resistance of young rabbits to Rabbit Haemorrhagic Disease (RHD).

    Science.gov (United States)

    Marques, Raquel M; Teixeira, Luzia; Aguas, Artur P; Ribeiro, Joana C; Costa-e-Silva, António; Ferreira, Paula G

    2014-02-04

    Rabbit Haemorrhagic Disease (RHD) is caused by a calicivirus (RHDV) that kills 90% of infected adult European rabbits within 3 days. Remarkably, young rabbits are resistant to RHD. We induced immunosuppression in young rabbits by treatment with methylprednisolone acetate (MPA) and challenged the animals with RHDV by intramuscular injection. All of these young rabbits died within 3 days of infection due to fulminant hepatitis, presenting a large number of RHDV-positive dead or apoptotic hepatocytes, and a significant seric increase in cytokines, features that are similar to those of naïve adult rabbits infected by RHDV. We conclude that MPA-induced immunosuppression abrogates the resistance of young rabbits to RHD, indicating that there are differences in the innate immune system between young and adult rabbits that contribute to their distinct resistance/susceptibility to RHDV infection.

  18. Beneficial effect of lantibiotic nisin in rabbit husbandry.

    Science.gov (United States)

    Lauková, Andrea; Chrastinová, Lubica; Plachá, Iveta; Kandričáková, Anna; Szabóová, Renáta; Strompfová, Viola; Chrenková, Mária; Cobanová, Klaudia; Zitňan, Rudolf

    2014-03-01

    Nisin is a bacteriocin marketed as Nisaplin. The aim of our work was to test its in vivo effect in a rabbit model; its effect on phagocytic activity (PA) and morphometry has not so far been studied. Post-weaning rabbits (48), 5 weeks old (both sexes, Hycole breed), were divided into the experimental (E) and the control groups (C), 24 animals in each. They were fed a commercial diet with access to water ad libitum. Rabbits in E had nisin additionally administered to their drinking water (500 IU-20 μg per animal/day) for 28 days. The experiment lasted 42 days. On day 28, significant decrease in coagulase-positive (CoPS) staphylococci and coliforms was noted (p < 0.01) in faeces of group E compared with C. Pseudomonads and clostridiae were also significantly reduced (p < 0.001; p < 0.05) and slight decrease was also in CoNS and enterococci. On day 42, coliforms were still significantly reduced (p < 0.001) in faeces; slight decrease in CoPS and pseudomonads was noted. In the caecum, significant decrease in pseudomonads (p < 0.05) was noted on day 28; slight decrease in coliforms. In the appendix slight decrease in coliforms, pseudomonads was obtained on both days. PA was increased significantly in E on days 28, 42 (p < 0.001). Biochemical parameters were not influenced; nor were volatile fatty acids or lactic acid in the chymus. Nisin application did not evoke oxidative stress. In group E, an increase in average body weight gain (about 9.4 %) was noted. The villus height/crypt depth ratio was not influenced; that is, resorption surface and functionality of mucosa were not influenced.

  19. White Rabbit Status and Prospects

    CERN Document Server

    Serrano, J; Cattin, M; van der Bij, E; Wlostowski, T; Daniluk, G; Lipinski, M; Beck, D; Hoffmann, J; Kreider, M; Prados, C; Rauch, S; Terpstra, W W; Zweig, M

    2014-01-01

    The White Rabbit (WR) project started off to provide a sequencing and synchronisation solution for the needs of CERN and GSI. Since then, many other users have adopted it to solve problems in the domain of distributed hard realtime systems. The paper discusses the current performance of WR hardware, along with present and foreseen applications. It also describes current efforts to standardise WR under IEEE 1588 and recent developments on reliability of timely data distribution, finishing with an outline of future plans.

  20. Nonalcoholic steatohepatitis as a novel player in metabolic syndrome-induced erectile dysfunction: an experimental study in the rabbit.

    Science.gov (United States)

    Vignozzi, Linda; Filippi, Sandra; Comeglio, Paolo; Cellai, Ilaria; Sarchielli, Erica; Morelli, Annamaria; Rastrelli, Giulia; Maneschi, Elena; Galli, Andrea; Vannelli, Gabriella Barbara; Saad, Farid; Mannucci, Edoardo; Adorini, Luciano; Maggi, Mario

    2014-03-25

    A pathogenic link between erectile dysfunction (ED) and metabolic syndrome (MetS) is now well established. Nonalcoholic steatohepatitis (NASH), the hepatic hallmark of MetS, is regarded as an active player in the pathogenesis of MetS-associated cardiovascular disease (CVD). This study was aimed at evaluating the relationship between MetS-induced NASH and penile dysfunction. We used a non-genomic, high fat diet (HFD)-induced, rabbit model of MetS, and treated HFD rabbits with testosterone (T), with the selective farnesoid X receptor (FXR) agonist obeticholic acid (OCA), or with the anti-TNFα mAb infliximab. Rabbits fed a regular diet were used as controls. Liver histomorphological and gene expression analysis demonstrated NASH in HFD rabbits. Several genes related to inflammation (including TNFα), activation of stellate cells, fibrosis, and lipid metabolism parameters were negatively associated to maximal acetylcholine (Ach)-induced relaxation in penis. When all these putative liver determinants of penile Ach responsiveness were tested as covariates in a multivariate model, only the association between hepatic TNFα expression and Ach response was confirmed. Accordingly, circulating levels of TNFα were increased 15-fold in HFD rabbits. T and OCA dosing in HFD rabbits both reduced TNFα liver expression and plasma levels, with a parallel increase of penile eNOS expression and responsiveness to Ach. Also neutralization of TNFα with infliximab treatment fully normalized HFD-induced hypo-responsiveness to Ach, as well as responsiveness to vardenafil, a phosphodiesterase type 5 inhibitor. Thus, MetS-induced NASH in HFD rabbits plays an active role in the pathogenesis of ED, likely through TNFα, as indicated by treatments reducing liver and circulating TNFα levels (T or OCA), or neutralizing TNFα action (infliximab), which significantly improve penile responsiveness to Ach in HFD rabbits.

  1. Effect of diet supplementation with live yeast (Saccharomyces cerevisiae on performance of rabbit does and their progenies

    Directory of Open Access Journals (Sweden)

    T. Belhassen

    2016-03-01

    Full Text Available A study was conducted to determine the effect of live yeast supplementation in the diet of rabbit does on their mortality and reproductive performance and the performance of their progeny. A total of 52 cross-bred rabbit does (New Zealand×Californian were divided into 2 groups differing in diet offered during 2 reproductive cycles and containing (group S; n=26 or not (group C; n=26 1 g of yeast (Actisaf Sc 47, S.I. LESAFFRE, France/kg of feed. Natural mating was performed 11 d after kindling and kits were weaned at 28 d of age. Body weight of litters was measured at birth, 21 d and at 28 d of age (weaning. Mortality of kits and rabbit does was monitored daily, and fertility of rabbit does and viability rate of kits at birth were also determined. Weight and litter size at birth and at weaning, litter weight gain during lactation and length of gestation were similar between the 2 groups during the 2 cycles. The mortality of does during the experiment was higher in group C than in group S (27 vs. 4%; P<0.05. Fertility rate of rabbits does and viability rate of kits at birth were higher (P<0.05 in rabbits fed with the supplemented diet than those with the control diet during the second lactation. In the first cycle, kit mortality was lower in S group (15.5% than the C group (24.7% during the first 21 d (P<0.05. However, no difference was observed during the second lactation. In conclusion, our results suggest that the inclusion of yeast in the diet of rabbit does could trigger positive effects on the fertility and mortality of rabbit does, as well as on the viability rate of kits at birth.

  2. Effect of Stocking Density on Performance of Growing Rabbits in Semi-Humid Tropics

    Directory of Open Access Journals (Sweden)

    Iyeghe-Erakpotobor Grace, T.

    2005-01-01

    Full Text Available In order to evaluate the adoption possibility of Maize- Mucuna-Fertiliser (mineral System (MMFS, for soil fertilisation in the southern part of Togo, 52 farms from three representative villages of the region were analysed and classified into three types according to their adoption potential for the innovation: type A characterised by heads of farm being in majority land owners; type B whose head of farm are young, literate and in majority land tenants; and type C whose heads are old mainly land tenants. Participatory surveys, based on contingent ranking of the constraints, conducted with homogenous groups of farmers chosen according to three types of farm, helped to sort out the major constraints to the adoption of MMFS. These constraints, having a high degree of severity (≥ 45%, were in order of importance: the lack of money/loan, the land problem (lack of arable lands and insecurity on rented ones, the suppression of mixed cropping by the MMFS and the non availability of mucuna seed. For the farmers of type A, the non availability of mucuna seed and the lack of money/loan presented a degree of severity of 100% each. For those of type B and C, the land problem was the most important: it presented a degree of severity of 100% for the farmers of type B and 80% for those of type C. Sixty growing rabbits were housed at densities of 6.7, 10, 13.3, 16.7 and 20 rabbits/m2 in wood and poultry wire mesh cages for six weeks and fed a common diet of 22.6% CP and 2600 kcal/kg ME. Parameters measured were feed intake, weight gain, feed conversion ratio, body condition, fur condition and body bites. Stocking density significantly (P< 0.05 affected feed intake and weight gain but not feed conversion ratio of rabbits. Rabbits stocked at densities of 6.7, 10 and 13.3 rabbits/m2 had higher average daily gain than those stocked at higher densities. Feed intake was higher at higher densities. The trend shows a significant decrease in feed intake by individual

  3. Dietary Supplementation of Oregano and Sage Dried Leaves on Performances and Meat Quality of Rabbits

    Institute of Scientific and Technical Information of China (English)

    Luca Rotolo; Francesco Gai; Silvana Nicola; Ivo Zoccarato; Alberto Brugiapaglia; Laura Gasco

    2013-01-01

    The aim of this research was to evaluate the dietary supplementation 1% (w/w) of oregano and sage dried leaves on performances and meat quality of broiler rabbits. A feeding trial, which lasted 48 d, was carried out on 105 male Bianca Italiana rabbits randomly divided in seven groups and fed ad libitum. At the end of the trial ten animals per group were slaughtered and samples of dorsal muscle were taken in order to perform laboratory analysis. Mortality rate did not statistically differ between groups. Growth performances of animals fed diets supplemented with aromatic plants were higher (P<0.05) than those of animals of control group, whereas carcass parameters were not affected by treatments excepting for the slaughter weight that showed the same trend as growth performances. Meat quality traits, oxidative lipid stability and fatty acid proifle were not inlfuenced by aromatic plant supplementation. In conclusion, oregano and sage in form of dried leaves can be used in rabbit without adverse effects on performance, carcass characteristics and meat quality traits.

  4. Rice bran inclusion in the fruit and vegetable waste-based diets for fryer rabbits

    Directory of Open Access Journals (Sweden)

    S Prawirodigdo

    2004-10-01

    Full Text Available The aim of the study is to investigate the effect of rice bran inclusion in the fruit and vegetable waste (FVW-based diets on the growth performance of fryer rabbits. Thirty-six growing rabbits (New Zealand x Flemish Giant of about 1070.8 g initial body-weight were housed individually in the wire mesh cages and assigned to either one of a FVW-based diet containing 20% rice bran (RB 20%, 10% rice bran (RB 10% or zero rice bran (RB 0%. Thus, each treatment consists of 12 replicates. Data were collected for 28 days. Results showed that inclusion of rice bran in the diet significantly decreased (P<0.05 dry matter intake (2888, 2830 and 3095 g, for Diets RB 20%, RB 10%, and RB 0%, respectively. Average daily weight gain of the rabbits consuming RB 20% (23 g, RB 10% (25 g and RB 0% (33 g was significantly different (P<0.05. Inclusion of rice bran in the FVW-based diet significantly (P<0.05 affected the feed conversion ratio of the diets (3.4 versus 4.4 and 4.1, for RB 0% versus RB 20% and RB 10%, respectively. Consistently, the average carcass weight of rabbits consuming RB 0% (1140g was superior (P<0.05 to the carcass weight of rabbits fed RB 20% (1022 g or RB 10% (1046 g. Overall, inclusion of rice bran in the FVW based diet is not necessary. Simultaneously, use of FVW for rabbit is promising to avoid the accumulated fermenting FVW problem and produce healthy meat instantly for food.

  5. Doe productivity indices and sire effects of a heterogeneous rabbit ...

    African Journals Online (AJOL)

    IJAAAR

    Doe productivity indices are important in evaluating rabbit population since it influences the efficiency and profitability of rabbit ... Key words: Heterogeneous rabbit population, Doe productivity, Sire families). ..... Paris, France, 11-14. Cited in:.

  6. Cardiovascular physiology and diseases of the rabbit.

    Science.gov (United States)

    Pariaut, Romain

    2009-01-01

    This article reviews what is known about the diagnosis and management of cardiovascular diseases in the pet rabbit. Current knowledge is based on anecdotal reports, derived from research data using the rabbit as an animal model of human cardiovascular diseases, but most importantly canine and feline cardiology. It is likely that, as cardiovascular diseases are more often recognized, more specific information will soon become available for the treatment of the pet rabbit with cardiac disease.

  7. Welfare aspects in rabbit rearing and transport

    OpenAIRE

    Claudio Cavani; Massimiliano Petracci; Fabio Luzi; Marina Verga

    2010-01-01

    The review starts with the description of the rabbits’ (Oryctolagus cuniculus) main habits and the current situation concerning the rabbit husbandry and management systems, as well as their effects on the welfare of these animals. As far as the intensive rabbit husbandry systems are concerned, the main problems are related to the time since rabbits have been domesticated and their adaptive capacity and coping styles as respects the farming environment and management systems. Both these ...

  8. Rabbits' eye globe sonographic biometry.

    Science.gov (United States)

    Toni, Maria Carolina; Meirelles, Adriana Érica Wilkes Burton; Gava, Fábio Nelson; Camacho, Aparecido Antônio; Laus, José Luiz; Canola, Júlio Carlos

    2010-11-01

    To measure intraocular structures in New Zealand White breed rabbits (Oryctolagus cuniculus Linnaeus, 1758) using A-mode and B-mode ultrasound with a 20 MHz transducer. In this study, the eyes of 15 rabbits were evaluated for determination of intraocular measurements using an ophthalmic ultrasound unit able to operate in both A and B-modes. The distances from the cornea to the anterior capsule of the lens (D1), from the anterior capsule of the lens to the posterior capsule of the lens (D2), from the posterior capsule of the lens to the retina (D3) and the complete length of the eye, which corresponds to the distance from the cornea to the retina (D4) were taken. The mean values obtained were 2.70 mm (± 0.22 mm) for D1, 7.32 mm (± 0.40 mm) for D2, 7.10 mm (± 0.45 mm) for D3 and 17.12 mm (± 0.41 mm) for D4. Statistical analyses using the Student's t-test showed that there were no differences between the eyes. The study was feasible without the need of pharmacological restraint and yielded normal mean values for ocular sonographic biometry in rabbits. © 2010 American College of Veterinary Ophthalmologists.

  9. A Diet High in Saturated Fat and Sucrose Alters Glucoregulation and Induces Aortic Fatty Streaks in New Zealand White Rabbits

    OpenAIRE

    Yin, Weidong; Yuan, Zhonghua; Wang, Zongbao; Yang, Baotang; Yang, Yongzong

    2002-01-01

    A new and convenient animal model for studying peripheral vascular and coronary artery disease in diabetes was established in this study. Male New Zealand White rabbits weighing approximately 2 kg were divided into 2 groups: a normal control group fed standard laboratory chow and a diabetogenic diet–fed group received a high-fat/high-sucrose diet. The high-fat/high-sucrose diet (contained 10% lard and 37% sucrose) feeding was maintained for 6 months. Plasma total cholesterol, high-density lip...

  10. Fatty acid composition of phospholipids and esterified cholesterol of the blood plasma of rabbit under arginine acute pancreatitis

    Directory of Open Access Journals (Sweden)

    O. O. Hopanenko

    2015-04-01

    Full Text Available The content and fatty acid composition of phospholipids and esterified cholesterol were studied in the blood plasma of rabbits under acute arginine pancreatitis and its correction using linseed oil. It is established that the transport and anti-inflammatory functions of blood plasma deteriorates under acute arginine pancreatitis due to a decrease of the content of polyunsaturated fatty acids in phospholi­pids. The amount of cholesterol esterified with saturated and monounsaturated fatty acids increases in the blood plasma of rabbits. The concentration of phospholipids­ and esterified cholesterol is normali­zed and their fatty acid composition is improved in the lipid composition of the blood plasma of rabbits with acute arginine pancreatitis fed with linseed oil.

  11. Testování Fed modelu

    OpenAIRE

    Hříbalová, Pavlína

    2010-01-01

    Diploma Thesis focuses on Fed Model testing and its credibility on market data. The research is based on Gordon Model and Capital Asset Pricing Model (CAPM), it explains, what the basic features of the Fed Model are and describes its derivation from Gordon Model. The Thesis shows possible Fed Model limitation. It uses the US market, Great Britain and Germany 1979 -- 2011 data to demonstrate validity of the model. Eventually possible reasons of Fed Model development in period 2002 -- 2011 are ...

  12. Immunostimulant Effect of Egyptian Propolis in Rabbits

    National Research Council Canada - National Science Library

    Nassar, Somya A; Mohamed, Amira H; Soufy, Hamdy; Nasr, Soad M; Mahran, K. M

    2012-01-01

    The present experiment was conducted to study the effect of ethanolic extract of Egyptian propolis given alone or in combination with inactivated Pasteurella multocida vaccine on rabbits challenged...

  13. Attenuation of oxidative stress, inflammation, and endothelial dysfunction in hypercholesterolemic rabbits by allicin.

    Science.gov (United States)

    El-Sheakh, Ahmed R; Ghoneim, Hamdy A; Suddek, Ghada M; Ammar, El Sayed M

    2015-08-14

    Allicin, the active substance of garlic, exerts a broad spectrum of pharmacological activities and is considered to have potential therapeutic applications. The present study was designed to investigate the possible beneficial effects of allicin against oxidative stress, inflammation, and endothelial dysfunction in hypercholesterolemic rabbits. Male New Zealand white rabbits were used in this study. Rabbits randomly received 1 of the following treatments: normal chow diet for 4 weeks, 1% high cholesterol diet (HCD), HCD plus allicin (10 mg/kg/day), or HCD plus atorvastatin (10 mg/kg/day). Blood samples were collected at the end of experimental diets for measurement of serum total cholesterol (TC), triglycerides (TGs), high-density lipoprotein cholesterol (HDL-C), C-reactive protein (CRP), malondialdehyde (MDA), reduced glutathione (GSH), and superoxide dismutase (SOD). In addition, the aorta was removed for measurement of vascular reactivity, histopathological changes, intima/media (I/M) ratio, and immunohistochemical staining of both tumor necrosis-alpha (TNF-α) and nuclear factor (NF)-κB. HCD induced significant increases in serum TC, TGs, low-density lipoprotein cholesterol (LDL-C), CRP, and MDA. Moreover, HCD caused significant decrease in serum GSH and SOD. In addition, aortic relaxation response to acetylcholine (ACh) was impaired. Immunohistochemical staining of aortic specimens from HCD-fed rabbits revealed high expression levels of both TNF-α and the oxidant-induced transcription factor, NF-κB. Allicin supplementation significantly decreased serum MDA and CRP, increased serum HDL-C, GSH, and SOD levels while nonsignificantly affecting HCD-induced elevations in serum TC and LDL-C. Additionally, allicin significantly protected against HCD-induced attenuation of rabbit aortic endothelium-dependent relaxation to ACh and elevation in I/M ratio. This effect was confirmed by histopathological examination of the aorta. Moreover, allicin has substantially

  14. Is there a difference between hare syphilis and rabbit syphilis? Cross infection experiments between rabbits and hares

    NARCIS (Netherlands)

    Lumeij, J.T.; Mikalová, L.; Smajs, D.

    2013-01-01

    Abstract Cross infection of rabbits and hares with Treponema paraluiscuniculi from rabbits and the related microorganism from hares, which was provisionally named "Treponema paraluisleporis", revealed that T. paraluiscuniculi affects rabbits clinically, but only causes seroconversion in hares withou

  15. Impact of dietary fiber/starch ratio in shaping caecal microbiota in rabbits.

    Science.gov (United States)

    Zhu, Yanli; Wang, Chunyang; Li, Fuchang

    2015-10-01

    The objective of this experiment was to determine whether changing the dietary neutral detergent fiber (NDF)/starch ratio affected caecal microbiota when 4 different diets (diet A: 2.3 NDF/starch, diet B: 1.9, diet C: 1.4, diet D: 1.0) were formulated. A total of 200 weaned rabbits (35 days old, 50 per group) were used for the experiment, which started after an adaptation period of 7 days (i.e., day 42). Caecal contents were obtained from rabbits fed different NDF/starch diets at 52, 62, 72, and 82 days of life. The bacterial community structure was characterized by high-throughput 16S rRNA sequencing. Firmicutes, Actinobacteria, Synergistetes, and Tenericutes did not significantly change with diet or age. However, Bacteroidetes (P fiber) relative to diet D (high starch) in 52- and 62-day-old rabbits. Venn diagrams and heat map plot analyses revealed that the number of gut species shared between animals with different diet treatments increased with age. These results suggest that dietary fiber per starch ratios and age significantly alter the composition of caecal microbiota in growing rabbits.

  16. Influence of baroreflex on volume elasticity of heart and aorta in the rabbit.

    Science.gov (United States)

    Wronski, T; Seeliger, E; Persson, P B; Harnath, A; Flemming, B

    2002-03-01

    Optimal ventriculoaortic coupling includes tuning of elastic properties. The ratio of effective arterial elastance and left ventricular endsystolic elastance is often taken as a measure for mechanical and energetical efficiency. The present study determined the time course of ventricular and aortic volume elasticity (VE = dp/dV) throughout a complete heartbeat. This was achieved by using changes of eigenfrequency of two catheter-transducer systems under closed chest conditions in rabbits. Short-term VE modulation was studied by a baroreflex response, as induced by pressure changes applied to the carotid sinus. Long-term changes were studied in atherosclerotic rabbits (12 wk of high-cholesterol feeding). The time course and mean values of ventricular and aortic VE were changed by the baroreflex stimulus. Cholesterol feeding diminished the response. The degree of ventriculoaortic coupling, as quantified by VE(Aorta)/VE(Ventricle) ratio, varied during a single ejection period. The large span allows either maximal energetical efficiency or maximal stroke work. Although normal rabbits adjusted their ventriculoaortic coupling during baroreflex input, the cholesterol-fed rabbits failed to do so.

  17. Effect of ramipril on the electrophysiological characteristics of ventricular myocardium after myocardial infarction in rabbits.

    Science.gov (United States)

    Zhong, Ya; Cao, Ping; Tong, Chuanfeng; Li, Xia

    2012-05-01

    The current study aims to explore the effect of ramipril on the occurrence of ventricular arrhythmias and its possible mechanism after myocardial infarction (MI) in rabbits. A total of 24 rabbits were divided into three groups: the sham operation group (SHAM), the MI group, and the ramipril group (RAM). All groups were subjected to thoracotomy under sterile conditions; the MI and RAM groups underwent ligation of the left anterior descending coronary artery. On the second day after surgery, the RAM group was given ramipril (1 mg/kg per day). The rabbits in each group were fed for 12 weeks. The monophasic action potentials of the epicardium, mid-myocardium and endocardium in each group were, respectively, recorded before the MI and at 12 weeks after the MI. Meanwhile, the episodes of ventricular tachycardia or fibrillation (VT/VF) induced by procedure stimulations were counted, and the changes in L-type Ca flux (Ica-L) were recorded by means of the whole-cell patch-clamp technique. The episodes of VT/VF were decreased in the RAM group after MI. At 12 weeks after MI, the transmural dispersion of repolarization (TDR) in the MI group was prolonged significantly compared with the SHAM and RAM groups. The density of Ica-L in the MI group was significantly lower than that any other group. Ramipril manifestly decreases the incidence of VT/VF after MI in rabbits, and the mechanism may be associated with its inhibitory effect on electrical remodeling after MI.

  18. Impaired Cerebellar Maturation, Growth Restriction, and Circulating Insulin-Like Growth Factor 1 in Preterm Rabbit Pups.

    Science.gov (United States)

    Sveinsdóttir, Kristbjörg; Länsberg, John-Kalle; Sveinsdóttir, Snjólaug; Garwicz, Martin; Ohlsson, Lennart; Hellström, Ann; Smith, Lois; Gram, Magnus; Ley, David

    2017-10-04

    Cerebellar growth is impeded following very preterm birth in human infants and the observed reduction in cerebellar volume is associated with neurodevelopmental impairment. Decreased levels of circulating insulin-like growth factor 1 (IGF-1) are associated with decreased cerebellar volume. The relationship between preterm birth, circulating IGF-1, and key cell populations supporting cerebellar proliferation is unknown. The aim of this study was to evaluate the effect of preterm birth on postnatal growth, circulating IGF-1, and cerebellar maturation in a preterm rabbit pup model. Preterm rabbit pups (PT) were delivered by cesarean section at day 29 of gestation, cared for in closed incubators with humidified air, and gavage fed with formula. Control term pups (T) delivered by spontaneous vaginal delivery at day 32 of gestation were housed and fed by their lactating doe. In vivo perfusion-fixation for immunohistochemical evaluation of cerebellar proliferation, cell maturation, and apoptosis was performed at repeated time points in PT and T pups. Results show that the mean weight of the pups and circulating IGF-1 protein levels were lower in the PT group at all time points (p Preterm birth in rabbit pups is associated with lower circulating levels of IGF-1, decreased postnatal growth, and decreased cerebellar EGL proliferation and Purkinje cell maturation. The preterm rabbit pup model exhibits important characteristics of human preterm birth, and may thus be suitable for the evaluation of interventions aiming to modify growth and cerebellar development in the preterm population. © 2017 S. Karger AG, Basel.

  19. Stretchable Electrochemical Impedance Sensors for Intravascular Detection of Lipid-Rich Lesions in New Zealand White Rabbits

    Science.gov (United States)

    Cao, Hung; Yu, Fei; Zhao, Yu; Scianmarello, Nick; Lee, Juhyun; Dai, Wangde; Jen, Nelson; Beebe, Tyler; Li, Rongsong; Ebrahimi, Ramin; Chang, Donald S.; Mody, Freny V.; Pacella, John; Tai, Yu-Chong; Hsiai, Tzung

    2014-01-01

    Flexible electronics have enabled catheter-based intravascular sensing. However, real-time interrogation of unstable plaque remains an unmet clinical challenge. Here, we demonstrate the feasibility of stretchable electrochemical impedance spectroscopy (EIS) sensors for endoluminal investigations in New Zealand White (NZW) rabbits on diet-induced hyperlipidemia. A parylene C (PAC)-based EIS sensor mounted on the surface of an inflatable silicone balloon affixed to the tip of an interrogating catheter was deployed 1) on the explants of NZW rabbit aorta for detection of lipid-rich atherosclerotic lesions, and 2) on live animals for demonstration of balloon inflation and EIS measurements. An input peak-to-peak AC voltage of 10 mV and sweeping-frequency from 300 kHz to 100 Hz were delivered to the endoluminal sites. Balloon inflation allowed EIS sensors to be in contact with endoluminal surface. In the oxidized low-density-lipoprotein (oxLDL)-rich lesions from explants of fat-fed rabbits, impedance magnitude increased significantly by 1.5-fold across the entire frequency band, and phase shifted ~5 degrees at frequencies below 10 kHz. In the lesion-free sites of the normal diet-fed rabbits, impedance magnitude increased by 1.2-fold and phase shifted ~5 degrees at frequencies above 30 kHz. Thus, we demonstrate the feasibility of stretchable intravascular EIS sensors for identification of lipid rich lesions, with a translational implication for detecting unstable lesions. PMID:24333932

  20. Effect of dietary supplementation with glutamine and a combination of glutamine-arginine on intestinal health in twenty-five-day-old weaned rabbits.

    Science.gov (United States)

    Chamorro, S; de Blas, C; Grant, G; Badiola, I; Menoyo, D; Carabaño, R

    2010-01-01

    The effect of dietary supplementation with 1% l-glutamine and a combination of 1% l-glutamine and 0.5% l-arginine on intestinal health was examined in weaned rabbits. A basal diet was formulated to meet nutrient recommendations. Another 2 diets were formulated by adding 1% (as-fed basis) Gln or a mixture of 1% (as-fed basis) Gln + 0.5% (as-fed basis) Arg (Gln-Arg) to the basal diet. In Exp. 1, a total of 357 rabbits were blocked by litter and assigned at random to the experimental diet to determine mortality (119 per diet) and growth performance (35 per diet; from weaning at 25 to 56 d of age). Rabbits were fed the experimental diets for a 2-wk period and thereafter received a commercial diet. Rabbits weaned at 25 d (blocked by litter and assigned at random to diets) were slaughtered at 35 d and used to determine apparent ileal digestibility of DM, CP, and AA (Exp. 2, a total of 60 rabbits), intestinal morphology, N-aminopeptidase and myeloperoxidase intestinal activity, the expression of PPARgamma at the ileum and kidney, serum immunoglobulin in healthy and sick rabbits (Exp. 3, a total of 24 rabbits), and ileal and cecal microbial composition by PCR-RFLP (Exp. 4, a total of 45 rabbits). Dietary treatment did not affect ADG, ADFI, or G:F, during the entire fattening period. Supplementation with Gln reduced mortality during the first 2 wk and the whole fattening period from 18.5 to 8.4% (P = 0.023) and from 31.9 to 20.2% (P = 0.039), respectively, whereas no effect was detected for Arg supplementation. Among all the variables studied, the reduction on mortality due to Gln was related to a reduced intestinal colonization (Eimeria lesions) and changes on microbial ecosystem in the ileum and cecum, reducing the frequency of detection of Clostridium spp. (from 86.7 to 33.3%, P = 0.003) at the ileum, and Helicobacter spp. at the ileum (from 86.7 to 46.7%, P = 0.003) and at the cecum (from 86.7 to 46.7, P = 0.028), whereas no effect was detected for Arg supplementation

  1. Effects of gestational maternal undernutrition on growth, carcass composition and meat quality of rabbit offspring.

    Science.gov (United States)

    Symeon, George K; Goliomytis, Michael; Bizelis, Iosif; Papadomichelakis, George; Pagonopoulou, Olga; Abas, Zafeiris; Deligeorgis, Stelios G; Chadio, Stella E

    2015-01-01

    An experiment was conducted in order to evaluate the effects of gestational undernutrition of rabbit does on growth, carcass composition and meat quality of the offsprings. Thirty primiparous non lactating rabbit does were artificially inseminated and randomly divided in three treatment groups: Control (C; fed to 100% of maintenance requirements throughout gestation, n = 10), early undernourished (EU; fed to 50% of maintenance requirements during days 7-19 of gestation, n = 10) and late undernourished (LU; fed to 50% of maintenance requirements during days 20-27 of gestation, n = 10). During the 4th week of the gestation period, LU does significantly lost weight compared to C and EU groups (Ptotal litter size (alive and stillborn kits) was not different among groups (10.7, 12.8 and 12.7 kits in C, EU and LU groups, respectively). Kit birth weight tended to be lower in the LU group. During fattening, body weight and feed intake were not different among offsprings of the three experimental groups. Moreover, the maternal undernutrition did not have any impact on carcass composition of the offsprings in terms of carcass parts and internal organs weights as well as meat quality of L. lumborum muscle (pH24, colour, water holding capacity and shear values) at slaughter (70 days of age). Therefore, it can be concluded that the gestational undernutrition of the mother does not have detrimental effects on the productive and quality traits of the offsprings.

  2. Effect of feed restriction on rabbit meat quality of the Rex du Poitou(®).

    Science.gov (United States)

    Larzul, C; Thébault, R G; Allain, D

    2004-07-01

    Two orylag(®) rabbit genotypes, Castor and Chinchilla, feed restricted from 8 to 18 weeks of age at 130 g/d or fed ad libitum, were compared for growth, feed efficiency, carcass traits, and meat sensory characteristics. The total body electrical conductivity (ToBEC) value was also measured to predict meat quality. The feeding level was more restrictive for Castor than for Chinchilla rabbits (74% and 90%, respectively). As expected, growth, feed efficiency, carcass yield and adiposity were altered in both genotypes with feed restriction. For restricted animals, tenderness, juiciness, flavour, flouriness, stickiness and fatness were not affected by the genotype. Feed restriction only significantly decreased flouriness in the Castor genotype. Correlations between meat characteristics and sensory traits were low, and the ToBEC value was not correlated with organoleptic quality.

  3. Myxomatosis: some observations on breeding the European rabbit flea Spilopsyllus cuniculi (Dale) in an animal house

    Science.gov (United States)

    Sobey, W. R.; Menzies, W.; Conolly, Dorothy

    1974-01-01

    Rabbit fleas for use in Myxomatosis investigations have been successfully bred on rabbits in an animal house. The timing of emergence appeared to be governed by a biological timing control interacting with different forms of disturbance. Yield was found to be related to litter size, the time the doe and her kittens were removed from the nest, the number of fleas put onto a doe before littering and the mean ambient temperature to which the doe was exposed in the week pre-partum. The survival rate of fleas in storage was affected by temperature, the degree of crowding, moisture content of the containers, whether fleas were fed or unfed and the source of fleas in terms of emergence times. PMID:4526409

  4. Satin and their crossbred rabbit production

    Directory of Open Access Journals (Sweden)

    Bram Brahmantiyo

    2010-06-01

    Full Text Available Rabbit productivity in intensive management to be evaluated considering development in the rabbit farming were rapidly increased. Rex, Satin and their crossbred (Reza rabbits have been developed in Research Institute for Animal Production and productivity information of each breed can be used as basis for policy or model of cultivation and breeding in the community. Research conducted by evaluating the productivity of growth, carcass and the carcass proportion of the three strains of rabbit. Rex and satin growth were no different, and Reza was higher at 12-14 weeks of age. Carcass production comes from cutting the rabbit at the age of six months, this trait is strongly influenced by the weight of rabbits, and Reza gives the lowest performance compared to Rex and Satin. Rex, Satin and Reza were medium type rabbits with dual-purpose product (fur and meat. These rabbits showed the child's growth is good enough, to adapt the environment temperature and high food and have adequate carcass production.

  5. Congenital Transmission of Schistosoma japonicumin the Rabbit

    Institute of Scientific and Technical Information of China (English)

    QianBao-zhen; H.O.Bogh; M.V.Johansen; WangPeng-peng

    2005-01-01

    Fourteen pregnant rabbits were each infected with 300 cercariae of Schistosoma japonicum and divided into two groups.Group M (n =8)was infected during mid-gestation (the organogenetic stage)and group L (n=6)was infected during late-gestation (the post-organogenetic stage).Mother rabbits and rabbit kittens were killed 45-60 days after infection and perfused in order to obtain worm counts.Furthermore,faecal egg counts and tissue egg counts from livers were obtained from the mother rabbits as well as the rabbit kittens.All mother rabbits became infected harbouring 207.6+20.2 and 220.0+27.5 adult worms in group M and L,respectively.In groups M and L, 13.5%and 46.7% of the kittens were infected,respectively,In 12 of 14 litters at least one kitten was infected.Tne infected kittens harboured between one and three adult S.japonicum.The livers of the kittens infected with a worm pair displaced lesions,as a result of egg deposition.The results, therefore,show that congenital transmission of S.japonicum can occur in rabbits.The close anatomical resemblance between the rabbit and human placenta may be indicative of the presence of congenital transmssion of S.japomcum infection in humans.

  6. Viral skin diseases of the rabbit.

    Science.gov (United States)

    Meredith, Anna L

    2013-09-01

    This article describes the viral skin diseases affecting the domestic rabbit, the most important being myxomatosis. Transmission and pathogenesis, clinical signs, diagnosis, treatment, and control are described and the article will be of interest to veterinary practitioners who treat rabbits. Shope fibroma virus, Shope papilloma virus, and rabbitpox are also discussed. Copyright © 2013 Elsevier Inc. All rights reserved.

  7. Low-residue diet fed to rabbits induces histomorphological and biomechanical remodeling of small intestine

    DEFF Research Database (Denmark)

    Liu, Y; Zhao, J; Liao, D;

    2016-01-01

    . Segments from duodenum, jejunum and ileum were excised. The intestinal diameter and length were obtained from digitized images of the segments at preselected luminal pressure levels and at no-load and zero-stress states. Circumferential and longitudinal stresses (force per area) and strains (deformation...... confirmed that the wall thickness decreased in all three segments, which was primarily due to mucosal thinning (PFeeding the low...

  8. growth development in pre-pubertal female rabbits fed crude oil

    African Journals Online (AJOL)

    OMA

    ... was conducted to examine the effects of crude oil (Bonny Light Grade) on the ... that growth was significantly depressed (P≤0.05) in the crude oil treated diets ... However, few works ... The results in this study revealed a severe depression in.

  9. Novel Calicivirus Identified in Rabbits, Michigan, USA

    Science.gov (United States)

    Wise, Annabel G.; Bolin, Steven R.; Mullaney, Thomas P.; Kiupel, Matti; Maes, Roger K.

    2009-01-01

    We report a disease outbreak in a Michigan rabbitry of a rabbit calicivirus distinct from the foreign animal disease agent, rabbit hemorrhagic disease virus (RHDV). The novel virus has been designated Michigan rabbit calicivirus (MRCV). Caliciviruses of the Lagovirus genus other than RHDV have not been described in US rabbit populations. The case-fatality rate was 32.5% (65/200). Clinical signs included hemorrhage and sudden death, with hepatic necrosis. Analysis of viral RNA sequence from >95% of the viral genome showed an average similarity of 79% with RHDV. Similarity of the predicted MRCV capsid amino acid sequence ranged from 89.8% to 91.3%, much lower than the 98% amino acid similarity between RHDV strains. Experimentally infected rabbits lacked clinical disease, but MRCV was detected in tissues by PCR. We propose that MRCV primarily causes subclinical infection but may induce overt RHD-like disease under certain field conditions. PMID:19961675

  10. Feeding programs promoting daily feed intake stability in rabbit males reduce sperm abnormalities and improve fertility.

    Science.gov (United States)

    Pascual, J J; Marco-Jiménez, F; Martínez-Paredes, E; Ródenas, L; Fabre, C; Juvero, M A; Cano, J L

    2016-08-01

    Feeding programs promoting daily feed intake (DFI) stability in rabbit males could be useful to ensure successful coverage of their nutritional requirements and for continued production of quality semen. To evaluate two feeding systems designed to reduce DFI variability, 115 rabbit males at age 1.2 years were randomly assigned to three different treatments for 294 days: CS, animals fed ad libitum with a control diet (127-g starch and 281-g total soluble fiber [hemicellulose + soluble fiber] kg(-1) dry matter); SF, males fed ad libitum with diet enriched in soluble fiber (86-g starch and 330-g total soluble fiber kg(-1) dry matter); and R, animals fed with CS diet but daily restricted to maintenance requirements. Feed intake, body weight, body condition, and variability of DFI were controlled every 42 days, and individual semen volume and sperm motility, concentration, acrosome status, and abnormalities every 15 days. In six commercial farms, the number of females inseminated, pregnant and kindling, as well as the number of kits born alive, was registered for 15,893 inseminations with pooled semen from each treatment. DFI was significantly lower for R males than for the other treatments (on average, -12 ± 4 g/day; P rabbit males may be useful to fit their needs and provide a constant daily supply of nutrients, with some sperm morphologic characteristics being improved, as well as the fertility of their pooled semen. Copyright © 2016 Elsevier Inc. All rights reserved.

  11. Effect of purple loosestrife (Lythrum salicaria) diet supplementation in rabbit nutrition on performance, digestibility, health and meat quality.

    Science.gov (United States)

    Kovitvadhi, A; Gasco, L; Ferrocino, I; Rotolo, L; Dabbou, S; Malfatto, V; Gai, F; Peiretti, P G; Falzone, M; Vignolini, C; Cocolin, L; Zoccarato, I

    2016-01-01

    In this study, 160 Hycole weaned rabbits (35 days old) were randomly divided into four groups of 40. The rabbits were studied throughout a 54-day experimentation period in order to determine the impact of dietary supplementation from herbs composed of 0.2%, 0.4% dry ground Lythrum salicaria leaves (LS) and 0.3% Cunirel(®) (CR; a commercial herb mixture containing LS as the main ingredient) on performance, digestibility, health and meat quality. The basal diet was given to the control group. No significant differences were found in performance, 10 rabbits from each group were selected for evaluation regarding apparent digestibility. The rabbits fed the control diet and the diet with the low level of LS had a higher level of CP digestibility than did the animals that were supplemented with the high LS levels and CR (85.7% and 84.9% v. 84.0% and 84.0%, respectively; Pmicrobiota diversity. In conclusion, the results of this study indicated that supplementation of LS in rabbit diets leads to an increase in the total white blood cells, total VFA and acetic acid concentration, and a decrease in the ammonia levels, as well as the digestibility when CR and high level of LS were supplemented, without causing any adverse effects on other parameters.

  12. Effect of diet chestnut tannin supplementation on meat quality, fatty acid profile and lipid stability in broiler rabbits

    Directory of Open Access Journals (Sweden)

    Ivo Zoccarato

    2010-01-01

    Full Text Available This study investigated the effect of chestnut tannins on meat quality in broiler rabbits. 72 commercial hybrid rabbits (mean body weight 740 g, 32 days old were fed for 49 days with three diets containing 0%, 0.5% and 1.0% of a commercial chestnut wood extract (ENC®, Sil- vachimica srl, respectively. Eight rabbits per group were slaughtered at 12 weeks of age and at 24h post-mortem pH and colour were measured on the carcass. Moreover, both sides of m. longis- simus thoracis (LT were dissected. Left side was used for cooking losses whereas the other side was used for the determination of fatty acid profile and lipid oxidation. Data were statistically analyzed by one-way ANOVA. No differences were found in pH, colour and cooking losses, as well as the fatty acid profile of LT muscle and its relative health indexes. Concerning the antioxidant effect, the ENC shows a positive and significant effect at the inclusion level of 0.5%. In conclusion, the ENC has not undesirable side effects on the meat quality of rabbits, although further studies will be necessary to find the optimal diet inclusion level of ENC to elicit a stronger antioxidant effect in the rabbit meat.

  13. 青紫兰兔的生物净化技术探讨%Chinchilla Rabbit Biological Purification Technology

    Institute of Scientific and Technical Information of China (English)

    吴韬奋; 瞿伟; 黄国俊; 张瑞忠; 徐平

    2014-01-01

    Objective To establish the SPF Chinchil a rabbit populations, to meet the needs of various types of research. Methods Approach taken by Caesarean births method for biological purification Chinchil a rabbits, conducted on behalf of breast-fed rabbits with SPF level to achieve the SPF microbial level. Results Obtained Chinchil a rabbits 88, 74 survived, the survival rate was 84.1%. Conclusion Rabbits pre-production period to judge accurately, can improve the success rate of caesarean section.%目的:为建立SPF级青紫兰兔种群,满足各类研究的需要。方法用剖腹取胎的方法,对青紫兰种兔进行生物净化,用SPF级代乳兔进行喂养,使其微生物等级达到SPF级。结果共取得青紫兰仔兔88只,存活74只,存活率为84.1豫。结论种兔预产期判断准确,可提高剖腹产手术的成功率。

  14. Effects of feeding regime and fattening season on performance and production efficiency of small-scale rabbit farms.

    Directory of Open Access Journals (Sweden)

    Alma Llambiri

    2010-08-01

    Full Text Available Five small scale family farms with rabbits of local breed were included in the study. Two groups of rabbits per farm were fattened during summer and winter seasons. One group was fed in usual way with feeds produced in farm consisted mainly of alfalfa dry or fresh depending on seson and family residues. The feeding of the other group was daily supplemented with 25 g concentrate feedstuffs(17% protein bought in the market. Supplementation with concentrate feedstuffs showed to improve meat production from rabbits in both seasons. Live weight at the end of fattening was increased with 210 g and 360 g respectively during winter and summer season. The factors that affect the differences in live weight realized were feeding regime, sex of rabbit (P<0.001 and season of fattening (P<0.01. Approaching growth curves with theoretical one of Gomperz showed that concentrate adding in feed ration improved daily weight gain. It did not affect the dynamics of body weight change. It affects the gradient of quantity of daily gains of rabbits. Concentrate supplementation of daily feeding during fattening period increased up to 15 % the meat production as well as net income of the farm.

  15. FED baseline engineering studies report

    Energy Technology Data Exchange (ETDEWEB)

    Sager, P.H.

    1983-04-01

    Studies were carried out on the FED Baseline to improve design definition, establish feasibility, and reduce cost. Emphasis was placed on cost reduction, but significant feasibility concerns existed in several areas, and better design definition was required to establish feasibility and provide a better basis for cost estimates. Design definition and feasibility studies included the development of a labyrinth shield ring concept to prevent radiation streaming between the torus spool and the TF coil cryostat. The labyrinth shield concept which was developed reduced radiation streaming sufficiently to permit contact maintenance of the inboard EF coils. Various concepts of preventing arcing between adjacent shield sectors were also explored. It was concluded that installation of copper straps with molybdenum thermal radiation shields would provide the most reliable means of preventing arcing. Other design studies included torus spool electrical/structural concepts, test module shielding, torus seismic response, poloidal conditions in the magnets, disruption characteristics, and eddy current effects. These additional studies had no significant impact on cost but did confirm the feasibility of the basic FED Baseline concept.

  16. 9 CFR 354.124 - Quarantine of diseased rabbits.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Quarantine of diseased rabbits. 354... INSPECTION AND CERTIFICATION VOLUNTARY INSPECTION OF RABBITS AND EDIBLE PRODUCTS THEREOF Inspection Procedures; Ante-Mortem Inspections § 354.124 Quarantine of diseased rabbits. If live rabbits, which...

  17. Clinical aspects of lagomorph dental anatomy: the rabbit (oryctolagus cuniculus).

    Science.gov (United States)

    Crossley, D A

    1995-12-01

    The lagomorphs most commonly encountered as pets are rabbits. There are many breeds of domestic rabbit, varying from dwarf varieties with an adult weight of under one kilogram to giants weighing 10 kg. This article provides a working knowledge of the dental anatomy and physiology of rabbits so that veterinarians can interpret clinical and radiographic findings when investigating rabbits with suspected dental disease.

  18. Effects of substitution of soybean meal-alfalfa-maize by a combination of field bean or pea with hard wheat bran on digestion and growth performance in rabbits in Algeria

    Directory of Open Access Journals (Sweden)

    G. Lounaouci-Ouyed

    2014-06-01

    Full Text Available The aim of the trial was to study the effects of replacement of a soybean meal-alfalfa-maize based diet by a mixture of field bean (g/kg as fed: 257 crude protein [CP], 139 neutral detergent fibre [NDF] or pea (g/kg as fed: 203 CP, 122 NDF combined with hard wheat bran (g/kg as fed: 140 CP, 396 NDF and without supplementation of synthetic DL-methionine, on diet digestibility, growth and slaughter traits of growing rabbits. Three diets were formulated: a control diet mainly consisting of 15% of soybean meal, alfalfa and maize (SBM15 diet; g/kg as fed: 161 CP and 267 NDF and 2 experimental diets based on 26% of field bean (FB26 diet; g/kg as fed: 167 CP and 250 NDF or 30% of pea (P30 diet; g/kg as fed: 167 CP and 255 NDF as main protein source, and completed with hard wheat bran (34 and 40%, respectively for FB26 and P30 diets partly replacing alfalfa and maize. Diets were distributed ad libitum to 3 groups of 40 mixed-sex growing rabbits of Algerian white population, placed in collective cages (4 rabbits/cage from weaning (28 d, mean weight: 614±112 g until 77 d of age (slaughter. Faecal digestibility was measured between 42 and 46 d of age in 7 rabbits/group. Gross energy and crude protein digestibility coefficients were similar for SBM15 and FB26 diets (78.6 and 86.4%, respectively and lower for P30 diet (75.1 and 83.6%, respectively; P≤0.03. Treatments had no effect (P=0.12 on mortality rate, which was on av. 9.2%. Treatments did not affect growth performance from 28 to 77 d of age (30.8 g/d or feed intake (mean 94.6 g/d, but feed conversion ratio was higher for rabbits fed FB26 and P30 diets compared to those fed SBM15 diet (3.13 vs. 2.94; P=0.006. The dressing out percentages (mean 66.6% and the muscle/bone ratio (6.8 were similar for the 3 groups of rabbits. In conclusion, the substitution of soybean meal-alfalfa-maize by a combination of field bean or pea with hard wheat bran, without methionine supplementation, does not seem to affect

  19. Antithrombotic and Antiatherosclerotic Properties of Olive Oil and Olive Pomace Polar Extracts in Rabbits

    Directory of Open Access Journals (Sweden)

    Nektaria Tsantila

    2007-01-01

    Full Text Available Olive oil polar lipid (OOPL extract has been reported to inhibit atherosclerosis development on rabbits. Olive pomace polar lipid (PPL extract inhibits PAF activity in vitro and the most potent antagonist has been identified as a glycerylether-sn-2-acetyl glycolipid with common structural characteristics with the respective potent antagonist of OOPL. The aim of this study was to investigate the effect of PPL on early atherosclerosis development on rabbits and to compare it with the antiatherosclerotic effect of OOPL. OOPL and PPL inhibition potency, towards both PAF action and PAF binding, was tested in vitro on washed rabbit platelets. Consequently, rabbits were divided into three groups (A, B, and C. All groups were fed atherogenic diet for 22 days. Atherogenic diets in groups B and C were enriched with OOPL and PPL, respectively. At the end of the experimental time, rabbits were euthanized and aortic samples were examined histopathologically. OOPL and PPL inhibited PAF-induced aggregation, as well as specific PAF binding, with PPL being more potent. Free and bound PAF levels and PAF-AH activity were significantly elevated at the end of the experimental time. Plasma total cholesterol, HDL cholesterol, LDL cholesterol, and triglycerides levels were also found increased. Groups B and C exhibited significantly increased values of EC50 compared to group A. Histopathological examination revealed that the development of early atherosclerosis lesions in groups B and C were significantly inhibited compared to group A. Significant differences were noted in the early atherosclerosis lesions between groups B and C, thus indicating that PPL exhibit its anti-atherosclerotic activity by blocking PAF receptor. Specific PAF antagonists with similar in vitro and in vivo bioactivity to those that have been previously reported in OOPL exist in PPL.

  20. Zinc Regulates Lipid Metabolism and MMPs Expression in Lipid Disturbance Rabbits.

    Science.gov (United States)

    Xu, Chenggui; Huang, Zhibin; Liu, Lijuan; Luo, Chufan; Lu, Guihua; Li, Qinglang; Gao, Xiuren

    2015-12-01

    Lipid disturbance induced by high-fat diet is a worldwide problem, and it can induce inflammation and oxidative stress in vivo. Zinc is considered as an antioxidant, anti-inflammatory agent. Since matrix metalloprotease 2 (MMP2) and matrix metalloprotease 9 (MMP9)'s expressions are changed under many pathological conditions, we would like to know how zinc affects lipid metabolism and MMP2, MMP9's expressions in the lipid disturbance rabbits. Twenty-four male New Zealand white rabbits were randomly divided into four groups. Each group had six rabbits, and they were fed with regular diet, high-fat diet, high-fat diet+zinc, and regular diet+zinc separately for 12 weeks. High-fat diet induced lipid disturbance significantly which raised the level of aspartate aminotransferase (pzinc supplement reversed this phenomenon (pZinc did not reduce total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) (p>0.05), but it lowered triglyceride (TG) and raised high-density lipoprotein cholesterol (HDL-C) (pZinc also reduced high-sensitivity C-reactive protein (hs-CRP) (pZinc reduced the epicardial adipose tissue and alleviated the hepatic steatosis. Zinc suppressed MMP2 and MMP9's expressions in vivo, but it did not alleviate the aorta fatty streak's severity in the lipid disturbance rabbits. Zinc protected the liver, reduced TG, hs-CRP, and IL-6 and raised HDL-C in the lipid disturbance rabbits. Zinc suppressed MMP2 and MMP9's expressions in vivo, but it did not alleviate the severity of aorta fatty streak induced by the high-fat diet.

  1. Productive and reproductive performance of rabbits does as affected by bee pollen and/or propolis, inulin and/or mannan-oligosaccharides

    OpenAIRE

    Y.A. Attia; Bovera, F.; W.S. El-Tahawy; A. M. El-Hanoun; Al-Harthi, M. A.; H.I. Habiba

    2015-01-01

    The aim of the paper was to compare the effect of prebiotics (inulin and/or mannan-oligosaccharides, MOS) and bee products (bee pollen and/or propolis) on productive and reproductive performance of rabbit does. Seventy nulliparous V-line female rabbits were distributed among 7 groups. The groups were fed the same diet and received no supplements (control group), natural molecules (bee pollen and/or propolis) at 200 mg/kg body weight (BW) or prebiotics (inulin and/or MOS) at 35 mg/kg BW. Produ...

  2. Parasitic infections of wild rabbits and hares

    Directory of Open Access Journals (Sweden)

    Ilić Tamara

    2014-01-01

    Full Text Available The paper presents the most important parasitic infections of wild rabbits and hares, which harmful effect in this animal population is manifested as a gradual weakening of the immune system, reduction in fertility, weight loss and constant exhaustion. Order of Lagomorpha (hares or lagomorphs belongs to superorder of higher mammals which includes the family of rabbits (Leporidae which are represented in Europe as well as the family of whistleblowers (Ochotonidae which live only in North America and Northern regions of Asia. The most important representatives of Leporidae family are European hare (Lepus europeus and wild rabbit (Oryctolagus cuniculus. The most important endoparasitosis of hares and wild rabbits are: coccidiosis, encephalitozoonosis (nosemosis, toxoplasmosis, sarcocystosis, giardiasis, cryptosporidiosis, protostrongylosis, trichostrngylodosis, passalurosis, anoplocephalidosis, cysticercosis and fasciolosis. The most frequent ectoparasites of rabbits and wild hares are fleas, lice and ticks. Reduction in hare population, which is noticed in whole Europe including Serbia, is caused by changed living conditions, quantitatively and qualitatively insufficient nutrition, increased use of herbicides as well as various infectious diseases and the diseases of parasitic etiology. Since wild rabbits and hares pose a threat to health of domestic rabbits and people, knowledge of parasitic fauna of these wild animals is of extreme epizootiological and epidemiological importance.

  3. Welfare aspects in rabbit rearing and transport

    Directory of Open Access Journals (Sweden)

    Claudio Cavani

    2010-01-01

    Full Text Available The review starts with the description of the rabbits’ (Oryctolagus cuniculus main habits and the current situation concerning the rabbit husbandry and management systems, as well as their effects on the welfare of these animals. As far as the intensive rabbit husbandry systems are concerned, the main problems are related to the time since rabbits have been domesticated and their adaptive capacity and coping styles as respects the farming environment and management systems. Both these aspects have implications in the present and future of rabbit rearing for different purposes. Examples are given on the effects of different housing and management systems on rabbit welfare, as well as examples of the ethological, physiological and productive indicators used to evaluate these effects. Transportation and, more generally, preslaughter phases including catching, fasting and lairage at the abattoir are considered major stressors for farmed rabbits and might have deleterious effects on health, well-being, performance, and finally, product quality. A general statement of the recent scientific studies considering the effects of pre-slaughter factors on physiological and productive measurements are reported. Finally, some indications in order to improve rabbit welfare, already present at the European level, are also outlined, together with the European Food Safety Authority opinions.

  4. Newer antipsychotics and the rabbit syndrome

    Directory of Open Access Journals (Sweden)

    Masalehdan Azadeh

    2007-06-01

    Full Text Available Abstract Background Rabbit syndrome is a movement disorder that is associated with long-term exposure to neuroleptic medications. Of particular interest and importance is the risk of rabbit syndrome with exposure to the newer atypical antipsychotics. Our recent experience with such a case brought to light the importance of exploring this risk. Methods MEDLINE and PubMed (1972–2006 databases were searched for English language articles using the keywords rabbit syndrome, tardive dyskinesia, antipsychotic, extrapyramidal symptoms and side effects. A recent case study is used to expand upon the literature available on newer antipsychotics and rabbit syndrome. Results We reviewed papers that addressed the following aspects of rabbit syndrome 1 the clinical manifestations 2 prevalence and risk factors, 3 etiopathogenesis 4 older antipsychotics and rabbit syndrome 5 newer antipsychotics, 6 treatment options. Moreover, we report a case of RS in a 50 year old white female, diagnosed with bipolar I disorder, that, after the discontinuation of risperidone, developed involuntary movements of the mouth that were fine, rhythmic and rapid, along the vertical axis, and without involvement of the tongue. After the re-introduction of risperidone, the symptoms decreased in a few hours and disappeared after 3 days. Conclusion Eleven cases of rabbit syndrome have been documented since the implementation of newer antipsychotics. Future research is needed to better understand the etiopathogenesis of rabbit syndrome in psychiatric populations treated with the atypical antipsychotics. Understanding the differences and similarities of rabbit syndrome and tardive dyskinesia is crucial to the creation of a successful treatment paradigm.

  5. Bobcat attack on a cottontail rabbit

    Science.gov (United States)

    Biggins, D.E.; Biggins, D.M.

    2006-01-01

    We observed an attack by a bobcat (Lynx rufus) on a cottontail rabbit (Sylvilagus) that involved stealthy approach by the cat for >1 h, followed by a 12.3-s chase covering 116.0 m for the cat and 128.4 m for the rabbit. During the chase, the route of the cat from starting point to kill site was more direct than the semi-circular route of the rabbit. Stride lengths for the cat and total distance covered by the chase were longer than those previously reported for bobcats.

  6. Benign Rabbit Calicivirus in New Zealand.

    Science.gov (United States)

    Nicholson, Leila J; Mahar, Jackie E; Strive, Tanja; Zheng, Tao; Holmes, Edward C; Ward, Vernon K; Duckworth, Janine A

    2017-06-01

    The Czech v351 strain of rabbit hemorrhagic disease virus (RHDV1) is used in Australia and New Zealand as a biological control agent for rabbits, which are important and damaging introduced vertebrate pests in these countries. However, nonpathogenic rabbit caliciviruses (RCVs) can provide partial immunological cross-protection against lethal RHDV infection and thus interfere with effective rabbit biocontrol. Antibodies that cross-reacted against RHDV antigens were found in wild rabbits before the release of RHDV1 in New Zealand in 1997, suggesting that nonpathogenic RCVs were already present in New Zealand. The aim of this study was to confirm the presence of nonpathogenic RCV in New Zealand and describe its geographical distribution. RCV and RHDV antibody assays were used to screen serum samples from 350 wild rabbits from 14 locations in New Zealand. The serological survey indicated that both RCV and RHDV are widespread in New Zealand wild rabbits, with antibodies detected in 10 out of 14 and 12 out of 14 populations, respectively. Two closely related RCV strains were identified in the duodenal tissue from a New Zealand wild rabbit (RCV Gore-425A and RCV Gore-425B). Both variants are most closely related to Australian RCV strains, but with 88% nucleotide identity, they are genetically distinct. Phylogenetic analysis revealed that the New Zealand RCV strains fall within the genetic diversity of the Australian RCV isolates, indicating a relatively recent movement of RCVs between Australia and New Zealand.IMPORTANCE Wild rabbits are important and damaging introduced vertebrate pests in Australia and New Zealand. Although RHDV1 is used as a biological control agent, some nonpathogenic RCVs can provide partial immunological cross-protection against lethal RHDV infection and thus interfere with its effectiveness for rabbit control. The presence of nonpathogenic RCVs in New Zealand wild rabbits has been long hypothesized, but earlier attempts to isolate a New Zealand RCV

  7. Effect of dietary supplementation of spirulina (Arthrospira platensis and thyme (Thymus vulgaris on apparent digestibility and productive performance of growing rabbits

    Directory of Open Access Journals (Sweden)

    Zsolt Gerencsér

    2014-03-01

    Full Text Available The aim of this study was to evaluate the effect of dietary supplementation with spirulina (Arthrospira platensis or/and thyme (Thymus vulgaris on total tract apparent digestibility of nutrients and the performance of growing rabbits. At weaning (5 wk of age the rabbits were randomly allotted to 7 groups (42 rabbits/group, 3 rabbits/cage. Rabbits in the control group (C-C received a control pelleted feed throughout the experiment (5-11 wk of age without any supplementation (crude protein: 176 g CP/kg, neutral detergent fibre: 325 g NDF/kg. In the other groups, the control diet was supplemented with 5% spirulina (S, mainly in substitution of soybean meal, or 3% thyme (T, mainly in substitution of alfalfa meal or by both 5% S and 3% T (ST for the whole (5-11 wk of age; groups: S-S, T-T, ST-ST or part of the growing period (8-11 wk of age; groups: C-S, C-T, C-ST. Supplementations had no effect on apparent digestibility of dry matter, organic matter, acid detergent fibre, gross energy and digestible energy. The CP TTAD was lowest in rabbits fed the S diet, whereas it was highest in C fed rabbits, the other 2 treatments being intermediate (P<0.001. The starch TTAD was lowest for S fed rabbits (98.3% and highest for ST fed rabbits (99.4%, the other 2 dietary groups being intermediate (P<0.001. In contrast, the ether extract TTAD was higher in T than ST and C dietary groups (on av. 70.4 vs. 67.7% respectively; P<0.001, with S fed rabbits showing an intermediate value (69.1%. The NDF TTAD of the ST diet was lower than that of the other 3 groups (16.4 vs. 21.0% respectively; P<0.001. The TTAD of Ca reached the lowest value for the S diet (53.5% compared with the other 3 diets (on av. 59.1%; P<0.001. The S diet also had the lowest digestibility (P<0.001 for K and P,but in this case the C group always showed the highest values (P<0.001, with T and ST rabbits exhibiting intermediate results. Spirulina and/or thyme dietary supplementation had no effect on

  8. Identification of Rabbit Myostatin Gene Polymorphisms

    Directory of Open Access Journals (Sweden)

    T. I. Amalianingsih

    2015-08-01

    Full Text Available The existence of selection on the rabbits with potential for meat has only been seen from phenotypic aspects including performance and productivity, while the molecular genetic studies are still very rare. One of the candidate genes for meat production traits in rabbit is myostatin. Totally 50 blood samples of male rabbits from Rex, Satin, Reza (crossing from Rex and Satin, Flemish Giant and FZ3 (crossing from Flemish Giant and Reza breed were used at Indonesian Research Institute for Animal Production (IRIAP. Genetic polymorphism by Polymerase Chain Reaction – Restriction Fragment Length Polymorphism (PCR-RFLP method used FspBI restriction enzyme. PCR-RFLP data were analyzed by calculating allele and genotype frequencies. Sequencing was performed in rabbit with different genotypes which represents each of the samples. Genotype of AT had two cut points of the FspBI restriction enzyme at the base position of 508 bp and 444 bp. The cut point at the base position of 446 bp was site mutation base T became A. Genotype of TT had one cut point at the base position of 508 bp and no mutation site. Allele T had higher frequency than allele A and just Rex and Reza rabbit breeds had two alleles. The other rabbits (Satin, Flemish Giant and FZ3 only had one allele i.e., allele T. PCR - RFLP analysis of the MSTN|FspBI gene segments was polymorphic in Rex and Reza rabbit breeds. All of rabbit breeds in this study did not have AA genotype.

  9. Organophosphorus poisoning in two Rex rabbits.

    Science.gov (United States)

    Jones, J M

    1984-01-01

    A case of organophosphorus (OP) poisoning in two Rex rabbits is described. Three animals were diagnosed as having dermatitis characterised by pruritus and alopecia due to infestation with Cheyletiella parasitivorax. Two of the animals were dipped in 2% malathion solution: one died within 15 hours post-dipping, the other was euthanased subsequent to the onset of convulsions. A procedure for the future dipping of rabbits is suggested, and a recommendation is made for a lower concentration of malathion to be used.

  10. INFECTIOUS MYXOMATOSIS (SANARELLI) IN PREGNANT RABBITS

    Science.gov (United States)

    Sprunt, Douglas H.

    1932-01-01

    Pregnancy in rabbits alters the reactivity of the tissues to the virus of infectious myxomatosis. The livers of pregnant animals with the myxoma have a central acidophilic necrosis. Secondary lesions in the lungs are much more numerous and larger in the pregnant than in the non-gravid animals. In like manner the lesions in the spleen are more extensive in the pregnant rabbit. On the other hand the skin lesions of the pregnant animal are decreased in size. PMID:19870088

  11. [Dynamics of endoparasite infections in rabbits at different rearing regimes].

    Science.gov (United States)

    Nosal, Paweł; Kowal, Jerzy; Nowosad, Bogusław; Bieniek, Józef; Kowalska, Dorota

    2009-01-01

    This study was intended to evaluate the occurrence and seasonality of infection of coccidian and helminth parasites, in three rabbit warrens differently managed. Mothers stayed with their offspring on deep litter (farm A, 90 dams), in boxes cleaned weekly (farm B, 30 dams) or on slatted floor (farm C, 10 females), whereas all the young after weaning (1 to 5 months of age) were kept in cages with slatted floor. Animals were fed a complete balanced pelleted feed (warrens A and B) or traditional forage (herd C). Antiparasitic treatment involved coccidiostatic drugs. Robenidine (alternately with Lerbec in farm A) was added to food in the first two farms, whilst Sulfatyf mixed with water was given once after weaning in farm C. A total of 203 individual faecal samples from females of breeding stock, and 133 pooled samples from young animals, were collected on an annual cycle during 2007-2008 (February-January), and analyzed according to a modified concentration McMaster method. Coccidians were identified based on sporulated oocysts. A number of coccidian species (Eimeria perforans, E. media, E. magna, E. irresidua, E. exiqua, E. coecicola, and E. piriformis) were observed in all farms. The most pathogenic species--E. intestinalis was found both in farm A and B, whereas E. flavescens and E. stiedae--only in the former. The level of infection was high, especially in young rabbits, with the prevalence of 94.9 to 100% and mean intensity from 11,161 to 28,871 oocysts per 1 g of faeces (OPG) in a particular warren. The highest intensity of infection was observed in May, when the mean output increased to 29,454, 56,952, and 23,815 OPG in warrens A, B, and C, respectively. A nematode, Passalurus ambiguus, was detected in all searched farms, with the prevalence from 14.1% to 27.5%, depending on a farm. The species was more often seen in the first part of year. The other helminths (Trichostrongylus retortaeformis, Graphidium strigosum, and Trichuris leporis) were found only in

  12. Light colour preference of growing rabbits

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    Zsolt Szendrő

    2010-01-01

    Full Text Available The objective of the experiment was to evaluate the light colour preference of growing rabbits placed in a free-choice cage. The experiment was carried out on 128 Pannon White growing rabbits weaned at the age of 5 weeks and placed into blocks (2m2 of four cages. The rabbits could move freely among the four cages (0.5m2 each through swing doors. The cages differed only in the colour of the light applied (white, yellow, green or blue. The lighting schedule was 16L: 8D. From 6 until 10 weeks of age, infrared video recording was performed once a week (24 hours. The number of rabbits in each cage was counted every 15 minutes. Feed consumption was measured weekly. Between 6 and 10 weeks of age the rabbits significantly preferred white light (28.0%. The preference order was the following: yellow (26.3%, blue (23.4% and green (22.3% (P<0.001. No significant differences were recorded in the feed consumption among the cages. In conclusion, the cage preference of the rabbits was slightly affected by the light colour.

  13. Ameliorative potential of vitamin E on the impact of dietary fumonisin B1 on reproductive performance of female rabbits

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    Francis Ayodeji Gbore

    2017-06-01

    Full Text Available Fumonisin B1 (FB1, a contaminant of agricultural products, particularly maize worldwide is known to be consumed by farm animals and has been documented to cause various physiological responses in animals. A 15-week trial on the ameliorative potential of vitamin E on the negative impacts of FB_1 on reproductive performance of rabbits was conducted. Forty-nine female rabbits aged 16 to 18 weeks weighing 1.65 to 2.0 kg body weight were assigned to seven experimental feeding groups: the control group received a diet without FB_1, three groups were fed diets containing different concentrations of FB_1 at 2.5, 5.0 or 7.5 mg kg^(−1, and three further groups had diets containing FB_1 and vitamin E i.e., 2.5 mg FB_1 kg^(−1 + 100 mg vitamin E, 5.0 mg FB_1 kg^(−1 + 100 mg vitamin E, and 7.5 mg FB_1 kg^(−1 + 100 mg vitamin E. Data obtained on reproductive parameters - gestation length, litter size, kit weight as well as the kit crown-rump length, were analysed using ANOVA. Serum reproductive hormones - luteinizing hormone (LH, follicle stimulating hormone (FSH, prolactin, prostaglanding F_2α (PGF_2α, and estradiol (E_2 levels in rabbits fed diets containing 7.5 mg FB_1 kg^(−1 were significantly lower than those for all other treatments. Rabbits fed diets containing >= 5 mg FB_1 kg^(−1 had significantly (p=5.0 mg FB_1 kg^(−1 were significantly (p<0.05 lower than the weight gain observed in the other treatment groups. Does fed diets supplemented with vitamin E had significantly (p<0.05 higher milk yield compared with does on not supplemented diets, including the control. This study has shown that vitamin E supplementation of does counteracts the adverse impacts of FB 1 on reproductive hormones, gestation length, kit weight, and milk production in rabbits.

  14. Digestible fibre to starch ratio and protein level in diets for growing rabbits

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    Gerolamo Xiccato

    2010-01-01

    Full Text Available To evaluate the effect of digestible fibre (DF to starch ratio (0.8, 1.5, and 2.8 and protein level (15% and 16% on health status, digestive physiology, growth performance, and carcass traits, 246 rabbits weaned at 33 d were fed until slaughter (75 d six diets formulated according to a bi-factorial arrangement (3 DF to starch ratios by 2 protein levels. Growth performance and carcass quality at slaughter were not af- fected by treatments. Increasing DF to starch ratio did not modify dry matter digestibility (62.0% on average, while increased (P<0.001 DF digestibility (52.3 to 68.1%, stimulated caecal fermentation (total VFA: 56.0 vs 67.8 and 67.2 mmol/l; P=0.02 and changed VFA molar proportions. Increasing dietary protein increased digestibility of dry matter (P=0.02, crude protein (P<0.001 and digestible fibre (P<0.001 and increased cae- cal VFA production (P<0.01. The highest mortality (17.1% vs 1.5% average mortality of the other groups, P<0.001 was found in rabbits fed the diet with the lowest DF to starch ratio and the highest protein level.

  15. Caffeine blocks disruption of blood brain barrier in a rabbit model of Alzheimer's disease

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    Ghribi Othman

    2008-04-01

    Full Text Available Abstract High levels of serum cholesterol and disruptions of the blood brain barrier (BBB have all been implicated as underlying mechanisms in the pathogenesis of Alzheimer's disease. Results from studies conducted in animals and humans suggest that caffeine might be protective against Alzheimer's disease but by poorly understood mechanisms. Using rabbits fed a cholesterol-enriched diet, we tested our hypothesis that chronic ingestion of caffeine protects against high cholesterol diet-induced disruptions of the BBB. New Zealand rabbits were fed a 2% cholesterol-enriched diet, and 3 mg caffeine was administered daily in drinking water for 12 weeks. Total cholesterol and caffeine concentrations from blood were measured. Olfactory bulbs (and for some studies hippocampus and cerebral cortex as well were evaluated for BBB leakage, BBB tight junction protein expression levels, activation of astrocytes, and microglia density using histological, immunostaining and immunoblotting techniques. We found that caffeine blocked high cholesterol diet-induced increases in extravasation of IgG and fibrinogen, increases in leakage of Evan's blue dye, decreases in levels of the tight junction proteins occludin and ZO-1, increases in astrocytes activation and microglia density where IgG extravasation was present. Chronic ingestion of caffeine protects against high cholesterol diet-induced increases in disruptions of the BBB, and caffeine and drugs similar to caffeine might be useful in the treatment of Alzheimer's disease.

  16. Mannan oligosaccharides as growth promoter in finishing rabbit: effect on in vivo performance and carcass traits

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    Antonino Nizza

    2010-01-01

    Full Text Available Four groups each consisting in 440, 60 days old rabbits, were fed, respectively, four experimental diets: (1 MOS_0.5 (Bio-Mos® at 0.5 g/kg; (2 MOS_1.0 (Bio-Mos® at 1.0 g/kg; (3 MOS_ 1.5 (Bio-Mos® at 1.5 g/kg and (4 antibiotics (AGP, colistin sulphate 144 mg/kg; tylosin 100 mg/kg and oxytetracyclin 1000 mg/kg. Up to slaughter age (82 days of age mortality rate was recorded daily. For each group, 64 rabbits were controlled weekly for live weight to calculate daily weight gain (DWG. Feed intake (and, by consequence feed conversion ratio was measured, weekly, per group. At 82 days 16 rabbits per group were slaughtered and carcass traits were recorded. No differences were recorded among groups in live weight at different age and in daily weight gain but, in particular during the last week, AGP and MOS_0.5 groups showed higher feed intake and less favourable feed conversion ratio. MOS_1.0 group showed significantly higher incidence of empty gastro-intestinal tract but not differences were found for dressing out percentage. Perirenal fat showed a lower incidence in MOS than in AGP groups.

  17. Overexpression of ABCG1 protein attenuates arteriosclerosis and endothelial dysfunction in atherosclerotic rabbits

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    Martin Ungerer

    2012-06-01

    Full Text Available The ABCG1 protein is centrally involved in reverse cholesterol transport from the vessel wall. Investigation of the effects of ABCG1 overexpression or knockdown in vivo has produced controversial results and strongly depended on the gene intervention model in which it was studied. Therefore, we investigated the effect of local overexpression of human ABCG1 in a novel model of vessel wall-directed adenoviral gene transfer in atherosclerotic rabbits. We conducted local, vascular-specific gene transfer by adenoviral delivery of human ABCG1 (Ad-ABCG1-GFP in cholesterol-fed atherosclerotic rabbits in vivo. Endothelial overexpression of ABCG1 markedly reduced atheroprogression (plaque size and almost blunted vascular inflammation, as shown by markedly reduced macrophage and smooth muscle cell invasion into the vascular wall. Also endothelial function, as determined by vascular ultrasound in vivo, was improved in rabbits after gene transfer with Ad-ABCG1-GFP. Therefore, both earlier and later stages of atherosclerosis were improved in this model of somatic gene transfer into the vessel wall. In contrast to results in transgenic mice, overexpression of ABCG1 by somatic gene transfer to the atherosclerotic vessel wall results in a significant improvement of plaque morphology and composition, and of vascular function in vivo.

  18. Effects of type of dietary fat on indices of immune status of rabbits.

    Science.gov (United States)

    Kelley, D S; Nelson, G J; Serrato, C M; Schmidt, P C; Branch, L B

    1988-11-01

    We have compared the effects of dietary saturated and unsaturated fats of the n-6 and n-3 types on the immune status of male New Zealand white rabbits. Four groups of rabbits (n = 8) were fed purified diets containing one of the following fats (7.6% w/w, 23 kcal%) for 5 mo, hydrogenated soybean oil (HSO); safflower oil (SFO); linseed oil (LSO); or menhaden oil (MHO). In vitro proliferation of peripheral blood lymphocytes (PBL) cultured with T-cell mitogens was significantly higher in the LSO group than in the other three groups, and that in the HSO group was higher than in the MHO and SFO groups which were not different from each other. Proliferation of PBL in response to B-cell mitogens was significantly higher in the LSO group than in the SFO and MHO groups. In vitro proliferation of splenocytes (SPC) from the LSO group was higher than that from the other three groups only when SPC were cultured with T-cell mitogens. Serum antibody levels against bovine serum albumin were significantly higher in the LSO group than in the SFO group after second and third immunizations. Spleen weights, number of SPC or PBL, and delayed-type hypersensitivity were not different among the four dietary groups. Our data show that linseed oil diet enhanced several indices of immune status in rabbits.

  19. Adult rabbits acquire resistance to lethal calicivirus infection by adoptive transfer of sera from infected young rabbits.

    Science.gov (United States)

    Ferreira, P G; Dinís, M; Costa-E-Silva, A; Aguas, A P

    2008-02-15

    Calicivirus infection of adult rabbits induces the so-called rabbit haemorrhagic disease (RHD) that kills 90% or more of the infected animals; in contrast, young rabbits (up to 8-week-old animals) are resistant to the same infectious agent. We report that calicivirus inoculation of young rabbits induced moderate titres of antiviral antibodies. When these rabbits reached adulthood, a second calicivirus inoculation resulted in resistance to RHD and boosting of antibody titres in half of the rabbits. Adoptive transfer of sera from calicivirus-infected young rabbits to naïve adult rabbits conferred resistance to RHD. We conclude that calicivirus infection of young rabbits induces specific anti-calicivirus antibodies that will protect them from RHD when they reach adulthood.

  20. Impact of probiotic-supplemented diet on the expression level of lactate dehydrogenase in the leukocytes of rabbits.

    Science.gov (United States)

    Ghoneim, Magdy A E; Moselhy, Said S

    2014-04-01

    Probiotics are known as living, nonpathogenic microorganisms that colonize the intestine and provide benefit to the host. The present study aims to measure one important energy metabolism-related enzyme activity in blood of rabbits fed on probiotics of recommended concentration. In addition, it also aims for the evaluation of the expression level of lactate dehydrogenase (LDH) enzyme using reverse transcriptase-polymerase chain reaction (RT-PCR) technique. Two groups of rabbits are used: control group receiving normal standardized diet and the other probiotic-supplemented group receiving the same diet containing probiotic, namely, Mega acidophilus (200 million cfu/kg body weight/day) for 4 weeks. The obtained results revealed that the rabbits supplemented with probiotics showed a significant decrease in the levels of serum total cholesterol (TC), triacylglycerol, high-density lipoprotein cholesterol (HDL-c) and low-density lipoprotein cholesterol (LDL-c) when compared with control group. Risk factors detected by measuring TC/HDL-c and LDL-c/HDL-c ratios showed statistically significant decrease in probiotic-supplemented rabbits when compared with control group. In addition, blood glucose and total LDH activity were elevated in probiotic-supplemented rabbits when compared with control group. RT-PCR products of LDH-M gene produced two specific amplicons. One amplicon has the expected size of 243 bp from all samples of rabbits as revealed by GelPro software. The level of LDH-M expression was found to be increased in the probiotic-supplemented group. However, unexpected amplicons are produced at 586 bp in all the samples, which may be a dimeric form of the amplified region. It was concluded that this probiotic blend is beneficiary for the metabolic reactions of lipids in the body. Moreover, LDH expression level can be considered as a biomarker for the effect of probiotic and hence monitoring the metabolic changes as reflected from its administration.

  1. Effect of cation-anion balance in feed on urine pH in rabbits in comparison with other species.

    Science.gov (United States)

    Heer, F; Dobenecker, B; Kienzle, E

    2017-03-16

    In the present investigation, the impact of diet composition on urine pH in rabbits was compared with previous studies on rabbits, cats, dogs, pigs and horses. A total of 13 dwarf rabbits were fed six different diets with a cation-anion balance (CAB) between -39 and +320 mmol/kg dry matter (DM) using ammonium chloride (NH4 Cl) as an acidifier. CAB was calculated as follows: CAB (mmol/kg DM) = 49.9*Ca + 82.3*Mg +43.5*Na + 25.6*K - 59*P - 62.4*S - 28.2*Cl; minerals in g/kg DM. Urine, faeces and blood were collected. Urine pH ranged from 5.26 ± 0.22 at a CAB of -39 mmol/kg DM to 8.56 ± 0.24 at a CAB of +320 mmol/kg DM. A low CAB in the feed reduced blood pH and blood base excess significantly. Renal excretion of Ca, P, Na and Mg and water was significantly higher in rabbits eating acidifying diets. In comparison with other species, rabbits reacted to acidifying diets in a similar way as cats, dogs and pigs. Rabbits on a mildly alkalizing diet, however, had a trend to higher urine pH than other monogastric species on such diets (cats, dogs, pigs, horses).

  2. Rabbit haemorrhagic disease: advantages of cELISA in assessing immunity in wild rabbits (Oryctolagus cuniculus).

    Science.gov (United States)

    Zheng, Tao; Parkes, John P

    2011-12-15

    Rabbit haemorrhagic disease (RHD) is an acute fatal disease of domestic and wild European rabbits (Oryctolagus cuniculus) caused by RHD virus (RHDV). Accurate assessment of immunity is of great importance for the conservation and control of wild rabbits. We evaluated a competitive ELISA (cELISA) against isotype ELISAs for assessing the protective immunity against the disease by challenging 50 wild-caught rabbits with a lethal dose of RHDV. Death or survival to the challenge was used as a criterion to determine the performance characteristics of the assay for the assessment of immunity in rabbits. At 1:10 dilution, a serum exhibiting ≥ 25% inhibition (1:10(25)) was regarded as the presence of RHDV-specific antibodies. Eleven of 16 (68.8%) rabbits with antibodies at 1:10(25) (rabbit calicivirus, which interfered with isotype ELISAs, had little impact on the specificity of the cELISA for the diagnosis of RHDV infection. The presence of RHDV-specific antibody at 1:10(50) by the cELISA is a reliable indicator for the protective immunity. In contrast to isotype ELISAs, the cELISA is a valuable specific tool for monitoring the herd immunity to RHD for the conservation and management of wild rabbits in the field.

  3. Avaliação da qualidade do sêmen em coelhos alimentados com rações contendo diferentes fontes de óleos vegetais - DOI: 10.4025/actascianimsci.v26i1.1945 Semen quality evaluation in rabbit fed on rations containing different sources of vegetable oil - DOI: 10.4025/actascianimsci.v26i1.1945

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    Andrea Cristiane Michelan

    2004-04-01

    Full Text Available Foi avaliado o sêmen de 40 coelhos distribuídos em um delineamento experimental inteiramente casualizado com 4 tratamentos (ração sem a adição de óleo e rações contendo 3% de óleo de canola, 3% óleo de milho ou 3% óleo de soja e 10 repetições. As colheitas de sêmen foram realizadas 2 vezes por semana, durante 5 semanas, totalizando 10 colheitas por animal. Avaliou-se volume, pH, cor, vigor, motilidade espermática progressiva, concentração espermática por mm3 e por ejaculado, número de doses inseminantes e anormalidades espermáticas. Os animais que receberam ração com 3% de óleo de canola apresentaram maior volume de sêmen e maior quantidade total de espermatozóides no ejaculado (p Semen was evaluated in forty rabbit distributed in a completely randomized design, with four treatments (oil-free ration and rations with 3% of canola oil, 3% of corn oil or 3% of soybean oil and 10 replications. Ten semen collections were taken, twice a week, for five weeks. Parameters consisted of volume, pH, color, vigor, progressive motility, spermatozoa concentration/mm3, total spermatozoa per ejaculation, number of inseminating doses and abnormality rates. The animals receiving 3% canola oil rations produced a larger semen volume, higher total spermatozoa rates per ejaculation, and a greater number of inseminating doses (p < 0.05. Other oils, including canola, used in the rations failed to affect semen parameters such as volume, pH, color, vigor, spermatic progressive motility and abnormality rates.

  4. Maturation of the intestinal digestion and of microbial activity in the young rabbit: impact of the dietary fibre:starch ratio.

    Science.gov (United States)

    Gidenne, T; Debray, L; Fortun-Lamothe, L; Le Huërou-Luron, I

    2007-12-01

    The developmental changes of intestinal digestive potential and caecal microbial activity were described in suckling and weaned rabbits according to two feeding programmes. Two groups of thirteen litters were fed from 18 to 42 days old a "High" or a "Medium" NDF:starch ratio diet (resp. 2.7 vs 2.0, groups HL and ML) with similar protein and lipid levels, and from 42 to 70 days old the two groups were fed a "Low" NDF:starch ratio diet (1.7). From 25 to 32 days (weaning), the milk and solid feed intake were 22% and 41% higher in ML group (Pamylase (17-fold, Pamylase and maltase activities, that were higher in HL group (Pmicrobial activity of the rabbit evolved markedly between 3 and 5 weeks of age, and was weakly affected when the NDF:starch ratio decreased from 2.7 to 2.0.

  5. Overexpression of Vascular Endothelial Growth Factor in Restenotic Abdominal Aorta of Rabbits

    Institute of Scientific and Technical Information of China (English)

    尹瑞兴; 杨德寨; 吴海; 黄凯; 巫相宏; 陈宇明

    2002-01-01

    Objective To investigatethe expression of vascular endothelial growth factor (VEGF) in the wall of normal and restenotic abdominal aorta of rabbits. Methods Restenotic model was developed by balloon-injured abdominal aorta in eight male New Zealand White rabbits fed with a 2.0% cholesterol diet beginning two weeks before operation and continuing four weeks after procedure. At the end of 4 weeks after injury, the animals underwent total body perfusion fixation. Then, the abdominal aorta from iliac artery root to the diaphragm was harvested and post-fixed in 10 % neutral formalin for 16hours. Eight male animals fed with general diet were used for a normal control. The VEGF protein level in normal and restenotic abdominal aorta of rabbits was studied by means of immunohistochemistry. Results VEGF protein was detected in 5 (62.5 % ) of 8normal abdominal aorta, 3 showed faint staining, and the remaining 2 showed moderate VEGF expression.VEGF expression at the protein level was identified in all 8 restenotic specimens, 2 showed faint staining, 4showed moderate staining, and the remaining 2showed strong VEGF expression. In contrast to normal vessels, VEGF in restenotic specimens was distinctly expressed at sites that contained clustered macrophages and proliferating smooth muscle cells as well as endothelial cells. VEGF immunostaining was more extensive in restenotic specimens (2. 00 ± 0.76)than in normal vessels (0.82 ±0. 83, P < 0.01) .Microvessels were found in 7 of the 8 restenotic lesions, but only one lesion showed VEGF staining in endothelial cells of the microvessels. Conclusion VEGF expression is consistently more intense in sections of restenotic abdominal aorta than in those of normal abdominal aorta. The VEGF expressed by the smooth muscle cells and foamy macrophages in the restenotic arteries may act as a local and endogenous regulator of endothelial cell functions, including maintenance and repair of luminal endothelium, and formation of intimal

  6. NIRS PREDICTION FOR PROTEIN AND INTRAMUSCULAR FAT CONTENT OF RABBIT HIND LEG MEAT

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    Gy. Bázár

    2007-06-01

    Full Text Available The goal of this study was to develop calibration equations to predict the chemical composition of raw, homogenized rabbit meat by means of near infrared spectroscopy (NIRS. 44 Pannon White rabbits were housed in groups in three different pen types (16 anim./m2, and were fed the same diet. Another 45 animals were housed in cages (12 anim./m2 and fed by different feeding regimes. Rabbits were slaughtered at the bodyweight of 2.4-2.5 kg. Homogenized fresh and freeze-dried left total hind leg muscles were investigated by NIRS using a NIRSystem 6500 equipment with small ring cup sample holder. The ether extract and protein content of all samples were determined chemically. Samples 44 of housing experiment were applied in producing LOCAL calibration equations tested on the 45 samples from the separate feeding experiment. Coefficients of determination (R2 of the predictions were 0.89 and 0.99 for fat, 0.85 and 0.96 for protein in fresh and freeze-dried samples, respectively. Results are reassuring, because the equations were applicable, however the analyzed samples were from independent housing and feeding systems. Therefore the chemical compositions differed in the two datasets, i.e. 9.46%, and 11.79% for fat, 85.75% and 83.44% for protein content in calibration and prediction datasets, respectively. The average of NIRS predicted values for fat and protein was 11.36%, 83.88% or 11.54%, 83.45% when using fresh or freeze-dried samples, respectively.

  7. Curcumin attenuates adhesion molecules and matrix metalloproteinase expression in hypercholesterolemic rabbits.

    Science.gov (United States)

    Um, Min Young; Hwang, Kwang Hyun; Choi, Won Hee; Ahn, Jiyun; Jung, Chang Hwa; Ha, Tae Youl

    2014-10-01

    Curcumin, the yellow substance found in turmeric, possesses antioxidant, anti-inflammation, anticancer, and lipid-lowering properties. Because we hypothesized that curcumin could ameliorate the development of atherosclerosis, the present study focused on the effects and potential mechanisms of curcumin consumption on high-cholesterol diet-induced atherosclerosis in rabbits. During our study, New Zealand white rabbits were fed 1 of 3 experimental diets: a normal diet, a normal diet enriched with 1% cholesterol (HCD), or an HCD supplemented with 0.2% curcumin. At the end of 8 weeks, blood samples were collected to determine the levels of serum lipids, cytokines, and soluble adhesion molecule levels. Gene expression of adhesion molecules and matrix metalloproteinases (MMPs) in aortas were measured by quantitative real-time polymerase chain reaction and Western blot. Compared with the HCD group, rabbits fed an HCD supplemented with 0.2% curcumin had significantly less aortic lesion areas and neointima thickening. Curcumin reduced the levels of total cholesterol, triglyceride, low-density lipoprotein cholesterol, and oxidized low-density lipoprotein cholesterol in serum by 30.7%, 41.3%, 30.4%, and 66.9% (all P cholesterol levels. In addition, curcumin attenuated HCD-induced CD36 expression, circulating inflammatory cytokines, and soluble adhesive molecule levels. Curcumin reduced the mRNA and protein expression of intracellular adhesion molecule-1, vascular cell adhesion molecule-1, P-selectin, and monocyte chemotactic protein-1, and it inhibited HCD-induced up-regulation of MMP-1, MMP-2, and MMP-9. Our results demonstrate that curcumin exerts an antiatherosclerotic effect, which is mediated by multiple mechanisms that include lowering serum lipids and oxidized low-density lipoprotein, thus modulating the proinflammatory cytokine levels and altering adhesion molecules and MMP gene expression.

  8. Effect of hesperidin dietary supplementation on growth performance, carcass traits and meat quality of rabbits

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    P.E. Simitzis

    2014-06-01

    Full Text Available An experiment was conducted to examine the dose effects of hesperidin dietary supplementation on fattening rabbits’ growth performance, as well as carcass and meat quality characteristics. Forty-eight Hyla hybrid male weaned (35 d old rabbits were purchased and randomly assigned to 3 dietary groups of 16 rabbits each and fed diets supplemented with the antioxidant hesperidin at 0, 1 and 2 g/kg feed. At 80 d of age, the rabbits were slaughtered and samples of Longissimus lumborum (LL muscle were used to estimate meat quality traits. No significant differences were observed in body weight at the age of 80 d, feed conversion rate (35 to 80 d, or organ weights among the 3 groups. The pH, colour, percentage of released water, shear force values and intramuscular fat content of LL muscle were not significantly influenced by the dietary treatment. Hesperidin dietary supplementation at both levels reduced the polyunsaturated fatty acids (PUFAs, mainly arachidonic (C20:4n-6, docosapentaenoic (C22:5n-3 and eicosapentaenoic (C20:5n-3 (only at 2 g/kg, and PUFA/SFA ratio (P<0.01. Based on the malondialdehyde (MDA values, hesperidin inclusion did not influence meat antioxidant status during the 9-d refrigerated storage at 4°C. Thus, we may conclude that dietary supplementation with hesperidin at the selected concentration levels did not generally influence growth performance, carcass traits, meat quality or antioxidant capacity in fattening rabbits, although meat values for PUFAs appeared to be decreased.

  9. Testosterone protects from metabolic syndrome-associated prostate inflammation: an experimental study in rabbit.

    Science.gov (United States)

    Vignozzi, Linda; Morelli, Annamaria; Sarchielli, Erica; Comeglio, Paolo; Filippi, Sandra; Cellai, Ilaria; Maneschi, Elena; Serni, Sergio; Gacci, Mauro; Carini, Marco; Piccinni, Marie-Pierre; Saad, Farid; Adorini, Luciano; Vannelli, Gabriella B; Maggi, Mario

    2012-01-01

    Metabolic syndrome (MetS) and benign prostatic hyperplasia (BPH)/lower urinary tract symptoms (LUTS) are often associated. One of their common denominators is hypogonadism. However, testosterone supplementation is limited by concerns for potential prostatic side effects. The objective was to determine whether MetS-associated prostate alterations are prevented by testosterone supplementation. We used a previously described animal model of MetS, obtained by feeding male rabbits a high-fat diet (HFD) for 12 weeks. Subsets of HFD rabbits were treated with testosterone or with the farnesoid X receptor agonist INT-747. Rabbits fed a standard diet were used as controls. HFD-animals develop hypogonadism and all the MetS features: hyperglycemia, glucose intolerance, dyslipidemia, hypertension, and visceral obesity. In addition, HFD-animals show a prostate inflammation. Immunohistochemical analysis demonstrated that HFD-induced prostate fibrosis, hypoxia, and inflammation. The mRNA expression of several proinflammatory (IL8, IL6, IL1β, and TNFα), T lymphocyte (CD4, CD8, Tbet, Gata3, and ROR γt), macrophage (TLR2, TLR4, and STAMP2), neutrophil (lactoferrin), inflammation (COX2 and RAGE), and fibrosis/myofibroblast activation (TGFβ, SM22α, αSMA, RhoA, and ROCK1/ROCK2) markers was significantly increased in HFD prostate. Testosterone, as well as INT-747, treatment prevented some MetS features, although only testosterone normalized all the HFD-induced prostate alterations. Interestingly, the ratio between testosterone and estradiol plasma level retains a significant, negative, association with all the fibrosis and the majority of inflammatory markers analyzed. These data highlight that testosterone protects rabbit prostate from MetS-induced prostatic hypoxia, fibrosis, and inflammation, which can play a role toward the development/progression of BPH/LUTS.

  10. Effect of high fluoride and high fat on serum lipid levels and oxidative stress in rabbits.

    Science.gov (United States)

    Sun, Liyan; Gao, Yanhui; Zhang, Wei; Liu, Hui; Sun, Dianjun

    2014-11-01

    The purpose of this study was to explore the effects of high fluoride and high fat on triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), total antioxidant capacity (T-AOC), lipid peroxide (LPO) and malondialdehyde (MDA) in rabbits. A factorial experimental design was used, with two factors (fluoride and fat) and three levels. Seventy-two male rabbits were randomly assigned into nine groups according to initial weight and serum lipid levels. The rabbits were fed with basic feed, moderate fat feed or high fat feed and drank tap water, fluoridated water at levels of 50 and 100mgfluorion/L freely. Biological materials were collected after 5 months, and serum lipid, T-AOC, LPO, and MDA levels were then measured. Using these data, the separate and interactive effects of high fluoride and high fat were analyzed. High fluoride and high fat both increased serum levels of TC, HDL-C and LDL-C significantly (Pfluoride and high fat (Pfluoride and high fat had different effects on TG levels: high fat significantly increased TG levels (Pfluoride had nothing to do with TG levels (P>0.05). High fat significantly elevated LPO and MDA levels and lowered T-AOC levels in serum (Pfluoride significantly increased LPO and MDA levels in serum (Pfluoride on these indexes. In summary, high fluoride and high fat increased serum TC and LDL-C levels individually and synergistically, and this would cause and aggravate hypercholesterolemia in rabbits. At the same time, high fluoride and high fat both made the accumulation of product of oxidative stress in experimental animals. Copyright © 2014 Elsevier B.V. All rights reserved.

  11. Yucca schidigera extract can promote rabbit fecundity and ovarian progesterone release.

    Science.gov (United States)

    Štochmal'ová, Aneta; Földešiová, Martina; Baláži, Andrej; Kádasi, Attila; Grossmann, Roland; Alexa, Richard; Chrenek, Peter; Sirotkin, Alexander V

    2015-09-01

    The aim of the present in vivo study was to determine the effects of yucca powder extract added to the rabbit females feed mixtures on kindling and conception rate. Rabbit does of the experimental groups were fed with the standard diet enriched with supplement of yucca dry extract at doses of 5 g/100 kg feed (E1 group) or 20 g/100 kg feed (E2 group) for 50 days. In our preliminary in vivo results, we shown that conception rate was significantly higher in both experimental E1 and E2 groups (82.4% and 100.0%, respectively) than in the control group (47.1%). The kindling rate was also significantly higher in the experimental groups (70.6% and 100.0%, respectively) than in the control group (41.2%). The differences between control and yucca-treated groups in the number of liveborn, stillborn, and weaned pups per doe were not statistically significant. To understand possible endocrine mechanisms of yucca action on fertility rate, we have examined the influence of yucca extract additions on the release of steroid hormones by isolated and cultured rabbit ovarian fragments. Yucca additions promoted release of progesterone (at dose of 1 μg/mL, but not at doses of 10 and 100 μg/mL). Yucca addition at these doses did not affect testosterone or estradiol release. Our observations show the stimulatory effect of yucca consumption on rabbit fecundity, which can be due to its direct stimulatory influence on ovarian progesterone but not on testosterone or estradiol output. Copyright © 2015 Elsevier Inc. All rights reserved.

  12. Anti-Atherogenic Activity of Ethanolic Fraction of Terminalia arjuna Bark on Hypercholesterolemic Rabbits

    Directory of Open Access Journals (Sweden)

    Saravanan Subramaniam

    2011-01-01

    Full Text Available Atherosclerosis which results from gradual deposition of lipids in medium and large arteries is a leading cause of mortality worldwide. Terminalia arjuna is a herb of Combretaceae family which contains hypolipidemic compounds and flavonoids with high antioxidative properties. This study was conducted to determine the effect of ethanolic fraction of T. arjuna on blood lipids and atherosclerosis in rabbits fed with high fat diet (HFD. Twenty New Zealand rabbits of either sex were randomly divided into five groups: the first two were normal diet group and HFD (21% fat group and the remaining three groups received high cholesterol diet supplemented with standard drug (Atorvastatin 10 mg kg−1 body weight, T. arjuna ethanolic fraction (100 and 200 mg kg−1 body weight, respectively. The concentration of total cholesterol (TC, low density lipoprotein (LDL cholesterol, triglycerides (TGs, very low density lipoprotein (VLDL cholesterol and high density lipoprotein (HDL cholesterol was determined in rabbits at the start of the experiment, at the 14th, 30th days and at the end of the study. Anti-atherogenic index was calculated from the lipid profile of the rabbits before sacrifice. At the end of the experimental period, the aorta was removed for assessment of atherosclerotic plaques. Results show that T. arjuna significantly decreases TC, LDL and TG levels and increases HDL and lessens atherosclerotic lesion in aorta (P < .05. Hence T. arjuna extract can effectively prevent the progress of atherosclerosis. This is likely due to the effect of T. arjuna on serum lipoproteins and its antioxidant and anti-inflammatory properties.

  13. Estimating Apparent Nutrient Digestibility of Diets Containing or Leaf Meals for Growing Rabbits by Two Methods

    Directory of Open Access Journals (Sweden)

    A. M. Safwat

    2015-08-01

    Full Text Available This study aimed to evaluate the nutrient digestibility of growing rabbits fed diets with different levels of either Leucaena leucocephala (LLM or Moringa oleifera (MOLM leaf meals and also to compare total collection and TiO2 marker methods for estimating digestibility. A total of 30 California growing rabbits (1.81±0.19 kg live weight on average were randomly distributed into five experimental groups of six rabbits each and were housed in individual cages. The groups were control, 30% LLM, 40% LLM, 30% MOLM, and 40% MOLM. All groups received pelleted diets for two weeks; diets also contained 4 g/kg titanium dioxide as dietary marker. Daily feed intake was recorded during the whole experimental period and total feces were collected daily and weighed individually during four days. The results showed that there were no difference (p>0.05 in feed, dry matter (DM, organic matter (OM, crude protein (CP, digestible energy, and crude fiber (CF intake between the control group and the other experimental groups. The apparent digestibility values of DM, OM, CP, CF, acid detergent fiber, and gross energy were the highest for control group (p = 0.001, meanwhile MOLM diets had generally higher nutrient digestibility coefficients than LLM diets. Increasing the inclusion level of leaf meal in the diet from 30% to 40% improved the digestibility of CF from 45.02% to 51.69% for LLM and from 48.11% to 55.89% for MOLM. Similar results for apparent nutrient digestibility coefficients were obtained when either total collection or indigestible marker method was used. In conclusion, the digestibility of MOLM containing diets were better than LLM diets, furthermore TiO2 as an external marker could be used as a simple, practical and reliable method to estimate nutrients digestibility in rabbit diets.

  14. Economics Of Rabbit Production In Abeokuta South Local ...

    African Journals Online (AJOL)

    Economics Of Rabbit Production In Abeokuta South Local Government Area Of Ogun State, Nigeria. ... Open Access DOWNLOAD FULL TEXT ... It involved the collection of primary data from a sample of SO rabbit farmers spread over 10 ...

  15. Review of experimental models: sinusitis in rabbits

    Directory of Open Access Journals (Sweden)

    André Coura Perez

    2014-10-01

    Full Text Available INTRODUCTION: In order to better understand the pathophysiology of rhinosinusitis, several attempts have been made to create the disease in an animal model. Among the studied rodents each has its advantages and disadvantages. Rabbits are considered more appropriate for studies that require surgical manipulation or invasive procedures. OBJECTIVES: To evaluate the most viable experimental model of rhinosinusitis in rabbits to be adopted in future studies. METHODS: An electronic search for studies with experimental models of rhinosinusitis in rabbits published in English and Portuguese between July of 1967 and January of 2013 was conducted in Medline, Pub Med, Cochrane, and CAPES databases, using the keywords "sinusitis", "rabbits", and "polyps". RESULTS: A total of 256 studies were retrieved, but in accordance with the inclusion and exclusion criteria, only ten studies were selected. Many different methods of response assessment were used in these studies. CONCLUSION: To date, there is no ideal experimental model for induction of acute or chronic rhinosinusitis in rabbits, but the rhinogenic model appears to be the most viable option for the continuity of studies of the disease.

  16. Genotoxic effects of copper sulfate in rabbits

    Directory of Open Access Journals (Sweden)

    Georgieva S.

    2013-01-01

    Full Text Available This study was carried out to determine the genotoxic effects of oral application of CuSO4 in rabbits by the chromosome aberration (CA and sister chromatid exchange (SCE tests. Ten male New Zealand rabbits (5 months old, weighing 3.5-4.0 kg were allocated into two groups. The first group received CuSO4 (5H2O in drinking water for 6 consecutive days. The second group was used as a control. On the 7th day, blood samples were taken from the ear marginal vein and the SCE and CA tests in peripheral lymphocytes were used as genotoxicity and mutagenicity endpoints, respectively. Results showed a significant increase in the frequencies of the aberrant cells (7.4±0.24, P<0.001 and CA (chromatid fragments 3.2±0.37, chromosome fragments 4.2±0.37, P<0.001, and total aberrations (7.4±0.24, P<0.001 after the treatment with CuSO4 when compared with the control group. The level of SCE per cell in the CuSO4-treated rabbits (9.66±0.062 was significantly higher than in rabbits from the control group. These findings show that copper exhibits a genotoxic and mutagenic potential in rabbits.

  17. Effects of diets with Amaranthus dubius Mart. ex Thell. on performance and digestibility of growing rabbits

    Directory of Open Access Journals (Sweden)

    Edgar Molina

    2015-03-01

    Full Text Available The effects on performance and digestibility in growing rabbits were studied by comparing 3 diets containing increasing inclusion rates of amaranth (Amaranthus dubius Mart. ex Thell.: 0 (A0, 160 (A16 and 320 g/kg (A32 diet. Diets were formulated isoproteic and isocaloric to meet the nutrient requirements of growing rabbits. One hundred and thirteen weaned New Zealand White rabbits (mean±standard deviation weight: 760±102 g, individually caged, were randomly assigned to one of the 3  experimental diets. Rabbits were fed ad libitum from 35 to 87 d of age, and health status and performance traits were  onitored. The coefficients of total tract apparent digestibility of the diets were measured between 42 and 46 d of age in 12 rabbits per treatment. Amaranthus dubius contained 209 g/kg dry matter (DM of crude protein and 398 g/kg DM of neutral detergent fibre. There were no significant differences between treatments in weight gain (mean 21.6 g/d and live weight at the end of the fattening period (mean 1883 g. Daily feed intake was higher (P<0.05 in A0 than in A16 and A32 diets (85.4 vs. 73.7 and 69.9 g/d, respectively, and feed conversion rate improved with increased inclusion of A. dubius in the diet (from 3.84 to 3.28 for A0 and A32 diets, respectively; P<0.05. Health status was not affected by the amaranth inclusion rate. Total tract apparent digestibility showed high values, with no differences among diets except for ether extract. Thus, A. dubius could be considered as an alternative source of protein and fibre for rabbit feeding in tropical and subtropical regions.

  18. Effect of Dietary Cysteine Supplementation on Growing Performance, Pelt Quality and Some Serum Biochemical Parameters of Young Rex Rabbit

    Directory of Open Access Journals (Sweden)

    Tossou Myrlene Carine B

    2013-01-01

    Full Text Available The present study was conducted to evaluate the effects of dietary cysteine, a sulphur containing amino acid supplementation on growth performance, pelt quality and a number of serum biochemical parameters of young Rex Rabbit. One hundred and twenty Rex Rabbits aged 45 days were divided into five dietary treatment groups including one control group and 4 experimental groups. Each group was composed of 24 animals and was fed with different diets for 56 days corresponding to the fattening period. Diet was incorporated with 0% of cysteine (control group and 0,10%, 0,20%, 0,30% and 0,40% respectively for the experimental groups. At the end of the experiment, results showed that cysteine supplementation to the diet affected average daily gain, live weight,skin length, skin area and pelt weight at the late phase of 42 to 56 days (P0.05

  19. Effects of gestational maternal undernutrition on growth, carcass composition and meat quality of rabbit offspring.

    Directory of Open Access Journals (Sweden)

    George K Symeon

    Full Text Available An experiment was conducted in order to evaluate the effects of gestational undernutrition of rabbit does on growth, carcass composition and meat quality of the offsprings. Thirty primiparous non lactating rabbit does were artificially inseminated and randomly divided in three treatment groups: Control (C; fed to 100% of maintenance requirements throughout gestation, n = 10, early undernourished (EU; fed to 50% of maintenance requirements during days 7-19 of gestation, n = 10 and late undernourished (LU; fed to 50% of maintenance requirements during days 20-27 of gestation, n = 10. During the 4th week of the gestation period, LU does significantly lost weight compared to C and EU groups (P<0.05. At kindling, C does produced litters with higher proportions of stillborn kits (P<0.05 while the total litter size (alive and stillborn kits was not different among groups (10.7, 12.8 and 12.7 kits in C, EU and LU groups, respectively. Kit birth weight tended to be lower in the LU group. During fattening, body weight and feed intake were not different among offsprings of the three experimental groups. Moreover, the maternal undernutrition did not have any impact on carcass composition of the offsprings in terms of carcass parts and internal organs weights as well as meat quality of L. lumborum muscle (pH24, colour, water holding capacity and shear values at slaughter (70 days of age. Therefore, it can be concluded that the gestational undernutrition of the mother does not have detrimental effects on the productive and quality traits of the offsprings.

  20. Diagnosis of dental problems in pet rabbits (Oryctolagus cuniculus)

    OpenAIRE

    2008-01-01

    Dental problems are very common in pet rabbits. To establish a correct diagnosis of rabbit dental pathology, a general knowledge of normal dental anatomy and physiology is necessary. The specific anatomy and the most common pathologies of rabbit dentition are reviewed. Techniques for diagnosing dental abnormalities - such as clinical examination, radiography and computed tomography (CT) - are summarized. Finally two clinical cases of rabbits with dental pathologies are described.

  1. Rabbit meat processing: historical perspective to future directions

    OpenAIRE

    Massimiliano Petracci; Claudio Cavani

    2013-01-01

    In past centuries, because rabbits are relatively small, animals slaughtered for consumption were generally eaten immediately. However, since a single rabbit would offer little more product than could be consumed at one sitting, little effort was devoted to developing preserved rabbit products (such as salted or dried meat, sausages, etc.). For this reason, although there is a rich history of recipes using rabbit meat in the Mediterranean area, there are few traditional further-processed prod...

  2. SPF rabbits infected with rabbit hepatitis E virus isolate experimentally showing the chronicity of hepatitis.

    Science.gov (United States)

    Han, Jian; Lei, Yaxin; Liu, Lin; Liu, Peng; Xia, Junke; Zhang, Yulin; Zeng, Hang; Wang, Lin; Wang, Ling; Zhuang, Hui

    2014-01-01

    This study focused on investigating the pathogenesis seen in specific-pathogen-free (SPF) rabbits following infection with a homologous rabbit HEV isolate (CHN-BJ-rb14) and comparing it to that seen following infection with a heterologous swine genotype 4 HEV isolate (CHN-XJ-SW13). Three of the four animals inoculated with the homologous rabbit HEV became infected, exhibiting an intermittent viremia, obvious fluctuations of liver function biomarkers alanine aminotransferase (ALT) and aspartate aminotransferase (AST), and persistent fecal virus shedding throughout the nine month study. In addition, liver histopathology showed both chronic inflammation and some degree of fibrosis. Both positive and negative-stranded HEV RNA and HEV antigen expression were detected in liver, brain, stomach, duodenum and kidney from the necropsied rabbits. Inflammation of extrahepatic tissue (duodenum and kidney) was also observed. Three of the four rabbits inoculated with the heterologous genotype 4 swine HEV also became infected, showing similar levels of anti-HEV antibody to that generated following infection with the homologous virus isolate. The duration of both viremia and fecal shedding of virus was however shorter following infection with the heterologous virus and there was no significant elevation of liver function biomarkers. These results suggest that rabbit HEV infection may cause more severe hepatitis and prolong the course of the disease, with a possible chronic trend of hepatitis in SPF rabbits.

  3. Serological Survey for RHD Antibodies in Rabbits from Two Types of Rabbit Breeding Farms.

    Science.gov (United States)

    Fitzner, A; Niedbalski, W

    2016-09-01

    Seroprevalence studies of RHDV antibodies in domestic rabbits were conducted between 2008-2014. A total of 12,169 sera from the provinces of central, southern and south-east Poland, including 7,570 samples collected from mixed-breed rabbits reared in smallholder farms and nearly 4,600 sera taken mainly from unvaccinated rabbits kept in industrial farms, were examined using ELISA tests. Additionally, cross-reactivity of selected tested and control archival sera using both classic RHDV and RHDVa antigens was determined by HI assay. The overall seroprevalence was 13.3%. In rabbits with unkown history of immunisation or RHD infection which came from small farms, RHDV antibodies were detected in 6.1% ranging between 1.0% to 17.2% of animals. In rabbits of the same group, but with a declared vaccination status, or confirmed exposure to an infectious virus, or coming from exposed females, the seroprevalence ranged from 83% to 100%. Among unvaccinated meat rabbits aged 71 to 90 days from industrial farms, low (1.85%, 4.17%, 11%), medium (34%, 54%) or high rates (98.7%) of seropositivity were detected. The seroconversion recorded in adult vaccinated females from industrial farms was 70% and 95%. Generally, the antibody levels examined by ELISAs and HI were comparable. However, a number of sera from the rabbits from small farms, as well as archival sera, showed clear differences. Several-fold differences in antibody titers, evidenced mainly in the postoutbreak sera, indictaed the contact of animals with RHDVa antigen. The overall results of the survey revealed a great proportion of seronegative rabbits potentially highly susceptible to RHD infection. In combination with the emergence of a novel pathogenic RHD virus type (RHDV2), it poses a severe risk of a next wave of fatal disease cases spreading in the native population of domestic rabbits, especially in farms with a traditional system of husbandry.

  4. Effect of trichlorfon on selenium distribution in rabbits.

    Science.gov (United States)

    Kossakowski, S; Dziura, A

    1987-01-01

    The investigations were performed on 94 rabbits intoxicated with trichlorfon which were untreated or treated. The intoxicated rabbits were given per os Na75SeO3. Selenium distribution in different organs and tissues was examined radiometrically. In intoxicated untreated rabbits and in those at the beginning of treatment decreased selenium level was found.

  5. A Preliminary internet survey of pet rabbit owners’ characteristics

    Directory of Open Access Journals (Sweden)

    J.A. Oxley

    2015-12-01

    Full Text Available This study aimed to conduct a preliminary survey to investigate basic ownership factors, frequency of microchipping and insurance and views of pet rabbit owners  n these areas and general rabbit management. More specifically, we aimed to investigate whether owners possess insurance, whether their rabbits are microchipped, and owners’ views on the recommendations relating to rabbits (e.g. recommended enclosure sizes and the law. A questionnaire was designed and promulgated through social media sites and rabbit forums. A total of 1183 responses were received. Just over 29% of respondents sourced their rabbits through rescue centres. 73.9% (867/1174 of owners stated that they had no pet insurance for their rabbits. Concerning microchips, 78.3% (919/1173 of rabbits were not microchipped, while 21.7% (254/1173 were. This preliminary study found that the majority of individuals are of the opinion that the relevant law is insufficiently publicised. A more detailed study would be beneficial to investigate and provide further insight into rabbit owners and their views and concerns for rabbits. The results of such a study could help formulate rabbit-related information and guidelines which in turn could have a direct impact on pet rabbit welfare.

  6. Effects of Rosiglitazone on Rabbit Atherosclerosis

    Institute of Scientific and Technical Information of China (English)

    ZHAO Qing; WEI Meng; ZHAO Bing-hui

    2008-01-01

    We sought to validate magnetic resonance imaging (MRI) for documenting the effects of rosiglitazone on rabbit atherosclerosis and the possible mechanism by treatment on the lesions.24 New Zealand white rabbits were randomly divided into normal group,control group and treatment group.After 4 weeks,all rabbits underwent MRI.After examination,the number of circulating endothelial progenitor cells (EPCs) and level of lipid,glucose,nitric oxide (NO) and superoxide anion (O-2.) were measured.MRI demonstrated the regression of atherosclerotic lesions by rosiglitazone which has good correlation with histopathology.Treatment not only increased the level of EPCs and NO,but also raised the ability of inhibition of O-2..MRI is a promising noninvasive technology to detect the effects of therapeutic interventions.Rosiglitazone slows the progression of atherosclerosis that may in part attribute to its improvement of mobilization of EPCs and the reduced oxidative stress.

  7. Images in Rabbit, Run from Archetypal Perspective%Images in Rabbit,Run from Archetypal Perspective

    Institute of Scientific and Technical Information of China (English)

    付晓芳

    2016-01-01

    John Updike is regarded as one of the greatest American novelists in the 20th century. His Rabbit series brought him a worldwide reputation, especially the first one Rabbit, Run is considered as his magnum opus and has drawn the most concern from the critics. This thesis is intended to shed light on Rabbit, Run by applying some important archetypal terms, especially the archetypal images. By sorting out the employment of images, this thesis aims to prove Harry's plight and to dig out the theme of the empty mental condition of the whole society. Therefore, it hopes to provide a new way to interpret this novel.

  8. Teratogenic effect of formaldehyde in rabbits

    Directory of Open Access Journals (Sweden)

    A. A. Al–Saraj

    2009-01-01

    Full Text Available Thirty three pregnant rabbits were exposed to vapour of 10% formaldehyde (12 ppm throughout the gestation period to know its effect on newborns. The results showed no abortion or foetal mortality but there were some anomalies (23.8% among the newborns rabbits which includes: meromelia (6.8%, encephalocele (6.1%, Oligodactyly (4.1%, Umbilical hernia (3.4% and Short tail (3.4%; besides that small for date and decrease in the body weight of the newborns were also noticed. These findings suggest that formaldehyde is a teratogenic agent.

  9. Plasma PIVKA proteins in rabbits given warfarin.

    Science.gov (United States)

    Zivelin, A; Rao, L V; Rapaport, S I

    1996-06-01

    The presence of partially carboxylated forms of the vitamin K dependent coagulation factors (PIVKA) was evaluated in the plasma of rabbits treated with warfarin. Excess antigen over activity as measured in rabbit specific assays was taken as evidence for PIVKA. Our data confirm a previous report of the absence of plasma PIVKA prothrombin. In contrast, plasma PIVKA factors VII, IX, and X were demonstrable. A striking excess of plasma factor IX antigen over activity was measured and a large fraction of the factor IX antigen persisted in the plasma after its adsorption with barium citrate.

  10. Chromate reduction by rabbit liver aldehyde oxidase

    Energy Technology Data Exchange (ETDEWEB)

    Banks, R.B.; Cooke, R.T. Jr.

    1986-05-29

    Chromate was reduced during the oxidation of 1-methylnicotinamide chlorine by partially purified rabbit liver aldehyde oxidase. In addition to l-methylnicotinamide, several other electron donor substrates for aldehyde oxidase were able to support the enzymatic chromate reduction. The reduction required the presence of both enzyme and the electron donor substrate. The rate of the chromate reduction was retarded by inhibitors or aldehyde oxidase but was not affected by substrates or inhibitors of xanthine oxidase. These results are consistent with the involvement of aldehyde oxidase in the reduction of chromate by rabbit liver cytosolic enzyme preparations.

  11. Detection of pathological lesions in slaughtered rabbits

    Directory of Open Access Journals (Sweden)

    Guido Grilli

    2010-01-01

    Full Text Available The slaughterhouse is considered an important control point for the monitoring of rabbit diseases. In our study, 59,440 rabbit carcasses were examined, but only 1% of pathological lesions were recorded at postmortem inspection. Mainly affected were tegumentary, digestive and urinary systems. The most consistent lesion was the subcutaneous abscess; nephritis, probably caused by Encephalitozoon cuniculi, was also frequent. Pathological alterations of the liver, classified as “necrotizing hepatitis” and localized at the caudate lobe, were observed for the first time.

  12. Sensitivity of rabbit fibrochondrocytes to mycoplasmas

    Directory of Open Access Journals (Sweden)

    Nascimento Carlos Manuel de Oliveira

    2002-01-01

    Full Text Available Primary cell culture from rabbit meniscus (fibrochondrocytes-FcrC was infected for 24 hours with different inocula (10² to 10(7 Colony Forming Units-CFU of Mycoplasma hominis PG-21, M. pneumoniae FH and 1428 or M. arthritidis PG-6. The severity of the different obtained cytophatic effects-CPE was inoculum, Mycoplasma species and strain dependant. These bacteria were recovered from all infected FcrC and the SP4 medium for mycoplasmas also caused toxic effect on the FcrC. It was concluded that rabbit fibrochondrocytes were sensitive to mycoplasma infection, as well as to the SP4 mycoplasma medium.

  13. Effect of dietary supplementation of Spirulina (Arthrospira platensis) and Thyme (Thymus vulgaris) on rabbit meat appearance, oxidative stability and fatty acid profile during retail display.

    Science.gov (United States)

    Dal Bosco, A; Gerencsér, Zs; Szendrő, Zs; Mugnai, C; Cullere, M; Kovàcs, M; Ruggeri, S; Mattioli, S; Castellini, C; Dalle Zotte, A

    2014-01-01

    The objective of this study was to evaluate the effect of Spirulina and Thyme supplementation on rabbit meat during retail display. At weaning 294 rabbits were allocated to 7 different treatments (42 rabbits/treatment). Rabbits of the control group (C) received a diet without any supplementation throughout the experiment (5-11 weeks of age). The other groups were fed diets containing 5% Spirulina (S), 3% Thyme (T) or both supplements (ST) for the whole trial (5-11 weeks; treatments S, T and ST), or for a part of the growing period (8-11 weeks; treatments C-S, C-T and C-ST). Colour parameters, pH, water holding capacity and drip loss were determined on fresh and stored Longissimus dorsi muscle of 5 rabbits/treatment. Spirulina- and Thyme-supplemented diets had a significant effect on redness and yellowness of Longissimus dorsi. Drip loss was significantly reduced in C-T and T groups that also showed the highest content of α-tocopherol and n-3 fatty acids content and the lower lipid oxidation.

  14. Experimental infection of wild-caught European rabbits (Oryctolagus cuniculus) with Sarcoptes scabiei from a naturally infected wild rabbit.

    Science.gov (United States)

    Millán, J; Casais, R; Colomar, V; Bach, E; Prieto, J M; Velarde, R

    2013-06-01

    Scabies was recently reported for the first time in the European wild rabbit, Oryctolagus cuniculus (Lagomorpha: Leporidae). We experimentally exposed 10 seronegative wild-caught rabbits to skin from a mangy wild rabbit. Serological, physiological, parasitological and histopathological changes were recorded. Three rabbits developed antibodies at 2-5 weeks post-infection (w.p.i.), two of which then developed lesions at 7 w.p.i. One of these had a small area of alopecia on the hind limb that healed naturally within 1 week; the other developed more extensive lesions restricted to the hind limbs (as typically observed in wild rabbits) that lasted until the rabbit died (12.5 w.p.i.). The third rabbit died of trauma 5 w.p.i. before developing any lesions. Antibodies in the healed rabbit disappeared from serum at 8 w.p.i., whereas antibody levels in the sick rabbit increased until its death. Disseminated intravascular coagulation and hepatic necrosis, probably arising from a concomitant infection with rabbit haemorrhagic disease virus, were the likely final cause of death in this rabbit. The mangy rabbit that served as a donor died of a multifocal fibrinosuppurative pneumonia that may have been secondary to the skin bacterial pyoderma.

  15. Effects of Moringa oleifera leaves as a substitute for alfalfa meal on nutrient digestibility, growth performance, carcass trait, meat quality, antioxidant capacity and biochemical parameters of rabbits.

    Science.gov (United States)

    Sun, B; Zhang, Y; Ding, M; Xi, Q; Liu, G; Li, Y; Liu, D; Chen, X

    2017-06-12

    This contribution reports the effects of Moringa oleifera leaves (MOLs) meal on the growth performances, nutrient digestibility, carcass trait, meat quality, antioxidant capacity and biochemical parameters of growing New Zealand white rabbits. The MOL was substituted for alfalfa meal at levels of 0, 10%, 20% and 30% to obtain respective diets MOL0, MOL10, MOL20 and MOL30. Each treatment was replicated five times with 10 rabbits per replicate. Results showed the average daily weight gain (ADWG) and feed conversion ratio (FCR) of rabbits fed MOL20 diet were significantly better (p oleifera leaves (MOL0, MOL10). The meat drip loss of rabbits fed with diet MOL10 was significantly lower (p oleifera leaves. No significant differences were found in the digestibility of crude fibre (CF), crude fat (EE), ash, crude protein (CP) and nitrogen-free extract (NFE) among the dietary groups. Moringa oleifera leaves also have a significant impact on serum albumin (ALB), low-density lipoprotein cholesterol (LDLC), triiodothyroxine (T3 ) and tetraiodothyroxine (T4 ) values and the activity of superoxide dismutase (SOD) and catalase (CAT) in serum and liver. The results indicated that M. oleifera leaves could be developed as a good feed source, and it not only could substitute for alfalfa meal well but also has a significant effect on growth performance, meat quality, antioxidant and biochemical parameters of rabbits. Journal of Animal Physiology and Animal Nutrition © 2017 Blackwell Verlag GmbH.

  16. Dietary supplementation of Digestarom® herbal formulation: effect on apparent digestibility, faecal and caecal microbial counts and live performance of growing rabbits

    Directory of Open Access Journals (Sweden)

    C. Celia

    2016-06-01

    Full Text Available The experiment aimed to study the effect of Digestarom® dietary inclusion (herbal formulation containing a mixture of essential oils, herbs, spices and extracts on apparent digestibility and digestive ecosystem of growing rabbits, as well as the effects of its supplementation before and after weaning on growth performance. At kindling, rabbit does and litters were divided into 2 dietary groups (51 does/group and fed either a control diet (C or a diet supplemented with 300 mg Digestarom®/kg diet (D until weaning, which occurred at 35 d (before weaning supplementation. Each group was further divided into 3 dietary groups: CC received the control diet and DD received the D diet from 5 to 12 wk of age, and DC were fed with D (from 5 to 8 wk of age and C diets (from 8 to 12 wk of age (after weaning supplementation; 54 kits/group. An in vivo digestibility trial and a faecal microbial count were carried out on growing rabbits that received only the C or D diets during the trial. The C group showed higher DM intake than D group (215 vs. 196 g/d; P<0.05. The faecal digestibility of ether extract (75.9 vs. 59.8%; P<0.001, cellulose (25.9 vs. 20.6%; P<0.05 and gross energy (51.8 vs. 49.1%; P<0.05 was higher for C than for D group, whereas that of starch (98.9 vs. 98.8%; P<0.001 and the digestible protein to digestible energy ratio (13.9 vs. 13.2 g digestible protein/MJ digestible energy; P<0.01 was the highest for rabbits fed D diet. Stomach and caecal pH, caecal and faecal microbial counts were independent of the dietary treatment. The only exception was the stomach pH in 8 wk-old rabbits, which had the lowest value in C rabbits (P<0.05. The D supplementation before weaning improved feed conversion ratio throughout the growing phase (4.3 vs. 4.4 for D and C, respectively; P<0.05, whereas significant differences in daily weight gain, feed conversion ratio and mortality were observed only in the first period after weaning. Based on the results obtained

  17. L-CARNITINE-INDUCED MODULATION OF PLASMA FATTY ACIDS METABOLISM IN HYPERLIPIDEMIC RABBITS

    Directory of Open Access Journals (Sweden)

    Frank Hernández Rosales PhD

    2006-02-01

    mejoramiento del metabolismo de las lipoproteínas. ABSTRACTThe present study was designed to examine whether the hipocholesterolemic effect of L-carnitine supplementation is related with lipoprotein fatty acid metabolism. Fatty acid compositional and cholesterol content changes were measured in lipoproteins of six different groups of rabbits. Group 1, rabbits fed a standard diet; group 2, rabbits fed standard diet plus L-carnitine 80 mg/kg bw; group 3, rabbits fed a 0.5 % cholesterol diet; group 4, rabbits fed a 0.5 % cholesterol diet plus L-carnitine 80 mg/kg b.w. These four groups were fed their diets during 126 days. Group 5 and 6 were fed the same diet as group 4 in a previous period of 126 days, and after this time, group 5 was fed the same diet as group 1, and group 6 fed the same diet as group 2, during a second period of 65 days.However, the progression of hypercholesterolemia was reduced 50 % by L-carnitine administration in those animals fed cholesterol diet. Fatty acid compositional changes in lipoprotein-cholesteryl esters were found in all groups of animals supplemented with L-carnitine. During the standard-fed period the saturated and unsaturated fatty acid ratio was increased in VLDL and HDL particles whereas was decreased in LDL. In the hyperlipidemia progression period the saturated to unsaturated fatty acid ratio in HDL fraction was slightly enhanced and in the VLDL+LDL modified particle was diminished. In the hyperlipidemia regression period, plasma cholesterol level was additionally reduced in a 33 % in the group 6; and the saturated to unsaturated fatty ratio had the same behaviour from that observed in the progression period for HDL and VLDL+LDL particles. A remarkable reduction (75% of aorta atherosclerotic plaques in the group 6 was found. From these results we concluded that L-carnitine, in this experimental model, induces an improved lipoprotein metabolism.

  18. Study on the Prevention of Rabbit Hair from Loss

    Institute of Scientific and Technical Information of China (English)

    刘世春; 张华鹏; 姚穆

    2001-01-01

    A new method is presented to solve the problem of loss of rabbit hair by using ES fiber blending with rabbit hair. ES fiber is used to bond the rabbit hair to prevent the rabbit hair from losing after heat setting. The factors affecting hair loss are heat setting temperature, rabbit hair/ES fiber blend ratio, fabric heating setting, twistsof yarn, etc. Temperature of heat setting and ES fiber content are the two key factors This method has almost no detrimental effect on the coziness of the fabric, which is better than other hair loss prevention methods.

  19. Possible interaction between myxomatosis and calicivirosis related to rabbit haemorrhagic disease affecting the European rabbit.

    Science.gov (United States)

    Marchandeau, S; Bertagnoli, S; Peralta, B; Boucraut-Baralon, C; Letty, J; Reitz, F

    2004-11-06

    Serological data on myxoma virus, rabbit haemorrhagic disease (RHD) virus and RHD-like viruses in juvenile rabbits (Oryctolagus cuniculus) trapped in 1995, 1996 and 1997 in two areas of France were analysed. For each disease, the effects of bodyweight, year, month and seropositivity for the other disease were modelled by using logistic regressions. In one area, a model including RHD seropositivity was selected to explain the myxoma virus seropositivity. Models including myxoma virus seropositivity were selected to explain the RHD seropositivity in both areas, and the odds of a rabbit being seropositive to both viruses were 5.1 and 8.4 times higher than the odds of a rabbit being seronegative to myxoma virus and seropositive to RHD. The year and bodyweight had significant effects for myxomatosis in one area and for RHD in both areas.

  20. Effects of rearing feeding programme on the young rabbit females’ behaviour and welfare indicators

    Directory of Open Access Journals (Sweden)

    E. Martínez-Paredes

    2015-09-01

    Full Text Available Restriction of young rabbit females during rearing is a widespread management technique that could have negative consequences on their welfare and behaviour. In the present work, a total of 24 young rabbit females aged 9 wk were used to evaluate 3 rearing feeding programmes until first parturition: CAL, fed ad libitum with a control diet [C: 11.0 MJ digestible energy (DE and 114 g digestible protein (DP per kg dry matter (DM]; CR, receiving the C diet restricted (140 g/d from 12 wk of age; and F, fed ad libitum with a low energy/high fibre diet [F: 8.7 MJ DE and 88 g DP per kg DM]. F females presented lower body weight than CAL and CR females at week 18 (–0.4 kg and –0.2 kg; P<0.05, but differences in body weight disappeared at parturition. Feeding programme affected the daily feed intake of young females during rearing and gestation periods (on av. of 2 periods: 140, 127 and 179 g DM/d, for CAL, CR and F females, respectively; P<0.001. Blood levels of glucose and insulin decreased with the age of rabbits (from 97 to 73 mg/dL for glucose and from 11 to 6 μUI/mL for insulin at 13 and 20 wk, respectively; P<0.001. Concentration of non-esterified fatty acids was higher in the blood of CAL females (+0.13 mmol/L compared to F; P<0.05, while corticosterone was higher in F females (+0.7 μg/dL compared to CAL; P<0.05. The type of feeding schedule affected the lying still and eating behaviour (P<0.01 of CR females, especially before and after feeding supply, as well as their behavioural stressed indicators (stereotypies; P<0.01, which were more frequent in CR females before feeding supply at 20 weeks of age. Therefore, ad libitum use of a low energy/high fibre diet is an adequate feeding programme for young rabbit females, which does not alter their behavioural patterns.

  1. Towards a unique and transmissible vaccine against myxomatosis and rabbit haemorrhagic disease for rabbit populations

    OpenAIRE

    Angulo, Elena; Bárcena, Juan

    2007-01-01

    Currently available vaccines against myxomatosis and rabbit hemorrhagic disease virus (RHDV) are not suited to immunise wild rabbit populations, as vaccines need to be delivered individually by conventional veterinary practices. As an alternative approach, research in Spain has focused on the development of a transmissible vaccine. A recombinant virus has been constructed based on a naturally attenuated myxoma virus (MV) field strain, expressing the RHDV capsid protein (VP60). Following inocu...

  2. Towards a unique and transmissible vaccine against myxomatosis and rabbit haemorrhagic disease for rabbit populations

    OpenAIRE

    Angulo, Elena; Bárcena, Juan

    2007-01-01

    Currently available vaccines against myxomatosis and rabbit hemorrhagic disease virus (RHDV) are not suited to immunise wild rabbit populations, as vaccines need to be delivered individually by conventional veterinary practices. As an alternative approach, research in Spain has focused on the development of a transmissible vaccine. A recombinant virus has been constructed based on a naturally attenuated myxoma virus (MV) field strain, expressing the RHDV capsid protein (VP60). Following inocu...

  3. Transcriptomic profiling of spleen in grass-fed and grain-fed Angus cattle

    Science.gov (United States)

    The grass-fed cattle obtain nutrients directly from pastures containing limited assimilable energy but abundant amount of fiber; by contrast, grain-fed steers receive a diet that is comprised mainly of grains and serves as an efficient source of high-digestible energy. Besides energy, these two type...

  4. Relationship of Breast-fed and Bottle-fed First Grade Students and I.Q.

    Science.gov (United States)

    Humphrey, Danette

    Previous studies have indicated some support for the hypothesis that breast feeding has a positive effect on intelligence and attainment among young children. This study examined the effects of breast-feeding versus bottle-feeding on the intelligence quotients (IQs) of first graders. A total of 26 breast-fed and 26 bottle-fed first graders from an…

  5. Prevalence and pathological study on rabbit hepatic coccidiosis in Taiwan.

    Science.gov (United States)

    Wang, J S; Tsai, S F

    1991-10-01

    Five breeds of rabbits, which included the New Zealand, Californian, Spot, Rex and Angora rabbit, were found from a survey of 1,152 rabbits in Taiwan. The prevalence of coccidia in young rabbits (weaning-2 months old) was 95% to 100%. Adult female rabbits usually acted as carriers within the farm and transmitted the parasite to young rabbits, which caused severe infection with clinical signs and even death. Parasitism of hepatic coccidia (Eimeria stiedai) in the rabbit led to severe mortality. Numerous and scattered white nodules about 0.1 to 0.5 cm in diameter were seen on the liver surface and dark greenish mucoid exudate was found in intestinal lumen. Histopathologic lesions included hyperplasia of the bile duct epithelium with different developmental stages of coccidia within. Oocysts could be seen in the lumen, and granuloma tissues encircle the bile duct with infiltration of inflammatory cells. The other organs were not infected.

  6. Encephalitozoonosis in household pet Nederland Dwarf rabbits (Oryctolagus cuniculus).

    Science.gov (United States)

    Valencakova, A; Balent, P; Petrovova, E; Novotny, F; Luptakova, L

    2008-05-31

    The paper presents the results of examination of 32 domestically bred rabbits, the breed Nederland Dwarf of Oryctolagus cuniculus, for the presence of Encephalitozoon cuniculi microsporidian species. The results of serological tests for E. cuniculi in 32 rabbits are reviewed along with other follow-up studies of clinical cases. Blood samples were taken from 7 asymptomatic rabbits and 25 rabbits showing neurological and ocular signs suggestive of encephalitozoonosis. In the asymptomatic group, 5 out of 7 rabbits were seropositive (71%). 16 rabbits with clinical diseases showed neurological sings, including torticollis, circus-like movements, loss of weight; 6 of them also showed ataxia, anorexia, asthenia of hind-limbs and 3 showed ocular signs. All 25 rabbits were seropositive. The spores of E. cuniculi were isolated from the faecal samples or kidneys and brain of an animal and subsequently were used for DNA isolation and PCR analysis.

  7. Ultrastructural researches on rabbit myxomatosis. Lymphnodal lesions.

    Science.gov (United States)

    Marcato, P S; Simoni, P

    1977-07-01

    Ultrastructural examination of head and neck lymph nodes in rabbits with spontaneous subacute myxomatosis showed fusion of immature reticuloendothelial cells which lead to the formation of polykarocytes. There was no ultrastructural evidence of viral infection of these polykaryocytes. Histiosyncytial lymphadenitis can be considered a specific lesion of myxomatosis.

  8. Rabbit trochlear model of osteochondral allograft transplantation.

    Science.gov (United States)

    To, Nhat; Curtiss, Shane; Neu, Corey P; Salgado, Christopher J; Jamali, Amir A

    2011-10-01

    Allografting and autografting of osteochondral tissues is a promising strategy to treat articular cartilage lesions in damaged joints. We developed a new model of fresh osteochondral allografting using the entire rabbit trochlea. The objective of the current study was to demonstrate that this model would achieve reproducible graft-host healing and maintain normal articular cartilage histologic, immunolocalization, and biochemical characteristics after transplantation under diverse storage and transplantation conditions. New Zealand white (n = 8) and Dutch belted (n = 8) rabbits underwent a 2-stage transplantation operation using osteochondral grafts that had been stored for 2 or 4 wk. Trochlear grafts harvested from the left knee were transplanted to the right knee as either autografts or allografts. Grafts were fixed with 22-gauge steel wire or 3-0 nylon suture. Rabbits were euthanized for evaluation at 1, 2, 4, 6, and 12 wk after transplantation. All grafts that remained in vivo for at least 4 wk demonstrated 100% interface healing by microCT. Trabecular bridging was present at the host-graft interface starting at 2 wk after transplantation, with no significant difference in cartilage histology between the various groups. The combined histology scores indicated minimal evidence of osteoarthritis. Immunostaining revealed that superficial zone protein was localized at the surface of all transplants. The rabbit trochlear model met our criteria for a successful model in regard to the ease of the procedure, low rate of surgical complications, relatively large articular cartilage surface area, and amount of host-graft bone interface available for analysis.

  9. Immunostimulant Effect of Egyptian Propolis in Rabbits

    Science.gov (United States)

    Nassar, Somya A.; Mohamed, Amira H.; Soufy, Hamdy; Nasr, Soad M.; Mahran, K. M.

    2012-01-01

    The present experiment was conducted to study the effect of ethanolic extract of Egyptian propolis given alone or in combination with inactivated Pasteurella multocida vaccine on rabbits challenged with a virulent strain of Pasteurella multocida. Fifty-six New-Zealand rabbits, 6–8 weeks old and non-vaccinated against pasteurellosis, were randomly divided into eight equal groups. The first group was kept as a control for the experiment. The other groups received different treatments with propolis extract, inactivated vaccine, or both. The experiment continued for seven weeks during which clinical signs, body weight, and mortality rate were monitored, and blood samples were collected weekly for evaluating the leukogram, serum biochemistry, and immune response in all groups of animals. At the end of the seventh week, the animals were subjected to challenge with a virulent strain of Pasteurella multocida. Two weeks later, tissue specimens were collected from different organs for histopathological examination. Results showed that rabbits of the groups treated with both propolis and the vaccine by different routes appeared healthy after challenge. It has been concluded that alcoholic extract of propolis administrated in combination with inactivated Pasteurella multocida vaccine has no adverse effects on the general health conditions and enhances immune response in rabbits. PMID:22654648

  10. Neutral detergent-soluble fiber improves gut barrier function in twenty-five-day-old weaned rabbits.

    Science.gov (United States)

    Gómez-Conde, M S; García, J; Chamorro, S; Eiras, P; Rebollar, P G; Pérez de Rozas, A; Badiola, I; de Blas, C; Carabaño, R

    2007-12-01

    The effect of neutral detergent-soluble fiber level on gut barrier function and intestinal microbiota was examined in weaned rabbits. A control diet (AH) containing 103 g of neutral detergent-soluble fiber/ kg of DM included alfalfa hay as main source of fiber. Another diet (B-AP) was formulated by replacing half of the alfalfa hay with a mixture of beet and apple pulp resulting in 131 g of soluble fiber/kg of DM. A third diet (OH) was obtained by substituting half of the alfalfa hay with a mix of oat hulls and a soybean protein concentrate and contained 79 g of soluble fiber/kg of DM. Rabbits weaned at 25 d and slaughtered at 35 d were used to determine ileal digestibility, jejunal morphology, sucrase activity, lamina propria lymphocytes, and intestinal microbiota. Suckling 35-d-old rabbits were used to assess mucosa morphology. Mortality (from weaning to 63 d of age) was also determined. Villous height of the jejunal mucosa increased with soluble fiber (P = 0.001). Rabbits fed with the greatest level of soluble fiber (BA-P diet) showed the highest villous height/ crypt depth ratio (8.14; P = 0.001), sucrase specific activity (8,671 mumol of glucose/g of protein; P = 0.019), and the greatest ileal starch digestibility (96.8%; P = 0.002). The opposite effects were observed in rabbits fed decreased levels of soluble fiber (AH and OH diets; 4.70, 5,848 mumol of glucose/g of protein, as average, respectively). The lowest ileal starch digestibility was detected for animals fed OH diet (93.2%). Suckling rabbits of the same age showed a lower villous height/crypt depth ratio (6.70) compared with the B-AP diet group, but this ratio was higher than the AH or OH diet groups. Lower levels of soluble fiber tended (P = 0.074) to increase the cellular immune response (CD8+ lymphocytes). Diet affected IL-2 production (CD25+, P = 0.029; CD5+CD25+, P = 0.057), with no clear relationship between soluble fiber and IL-2. The intestinal microbiota biodiversity was not affected by

  11. Proceedings of FED remote maintenance equipment workshop

    Energy Technology Data Exchange (ETDEWEB)

    Sager, P.; Garin, J.; Hager, E.R.; Spampinato, P.T.; Tobias, D.; Young, N.

    1981-11-01

    A workshop was convened in two sessions in January and March 1981, on the remote maintenance equipment for the Fusion Engineering Device (FED). The objectives of the first session were to familiarize the participants with the status of the design of the FED and to develop a remote maintenance equipment list for the FED. The objective of the second session was to have the participants present design concepts for the equipment which had been identified in the first session. The equipment list was developed for general purpose and special purpose equipment. The general purpose equipment was categorized as manipulators and other, while the special purpose equipment was subdivided according to the reactor subsystem it serviced: electrical, magnetic, and nuclear. Both mobile and fixed base manipulators were identified. Handling machines were identified as the major requirement for special purpose equipment.

  12. The European rabbit (Oryctolagus cuniculus), a source of zoonotic cryptosporidiosis.

    Science.gov (United States)

    Robinson, G; Chalmers, R M

    2010-12-01

    Cryptosporidium spp. have been found in the faeces of over 150 mammalian host species, but the risks to public health from wildlife are poorly understood. In summer 2008, the Cryptosporidium sp. rabbit genotype was identified as the aetiological agent in an outbreak of waterborne human cryptosporidiosis. The source was a wild rabbit that had entered a treated water tank. To establish current knowledge about Cryptosporidium spp. infecting lagomorphs, especially the host range and biological characteristics of the rabbit genotype, and the potential risks to public health that rabbits may pose in the transmission of zoonotic cryptosporidiosis, we undertook a literature and data review. The literature returned demonstrates that although the European rabbit (Oryctolagus cuniculus) has been the most widely studied lagomorph, few large scale studies were found. The prevalence of Cryptosporidium spp. in wild rabbit populations in the two large scale studies was 0.9% (95%CI 0.2-5.0) and 0.0% (95%CI 0.0-1.6). Neither study provided age nor sex profiles nor typing of Cryptosporidium isolates. The infecting Cryptosporidium species was confirmed in just four other studies of rabbits, all of which showed the rabbit genotype. Human-infectious Cryptosporidium species including Cryptosporidium parvum have caused experimental infections in rabbits and it is likely that this may also occur naturally. No published studies of the host range and biological features of the Cryptosporidium rabbit genotype were identified, but information was generated on the identification and differentiation of the rabbit genotype at various genetic loci. Both pet and wild rabbits are a potential source of human cryptosporidiosis and as such, good hygiene practices are recommended during and after handling rabbits or exposure to their faeces, or potentially contaminated surfaces. Water supplies should be protected against access by wildlife, including rabbits.

  13. Risk of zoonotic transmission of HEV from rabbits.

    Science.gov (United States)

    Lhomme, Sébastien; Dubois, Martine; Abravanel, Florence; Top, Sokunthea; Bertagnoli, Stéphane; Guerin, Jean-Luc; Izopet, Jacques

    2013-10-01

    Hepatitis E virus strains from rabbits indicate that these mammals may be a reservoir for HEVs that cause infection in humans. Further issues remain to be clarified, including whether the genotype of rabbit HEV differs from human and swine HEV genotype 3 and whether rabbit HEV can infect human and other animals. HEV was found in farmed rabbits in several geographic areas of China, in USA and more recently in France. The prevalence of antibodies against HEV was 36%, 57% and 55% in rabbits from Virginia (USA), Gansu Province and Beijing (China), respectively. HEV RNA was detected in 16.5% of serum samples from farmed rabbits in Virginia, 7.5% in Gansu Province and 7.0% in Beijing. HEV RNA was detected in 7% of bile samples from farmed rabbits and in 23% of liver samples from wild rabbits in France. The full-length genomic sequences analysis indicates that all the rabbit strains belong to the same clade. Nucleotide sequences were 72.2-78.2% identical to HEV genotypes 1-4. Comparison with HEV sequences of human strains circulating in France and reference sequences identified a human strain closely related to rabbit HEV. A 93-nucleotide insertion in the X domain of the ORF1 of the human strain and in all the rabbit HEV strains was found. Moreover, the ability of rabbit HEV to cause cross-species infection in a pig model has recently been demonstrated. Rabbit HEV can replicate efficiently in human cell lines. Collectively, these data support the possibility of zoonotic transmission of HEV from rabbits.

  14. Detection and localization of rabbit hepatitis e virus and antigen in systemic tissues from experimentally intraperitoneally infected rabbits.

    Science.gov (United States)

    Mao, Jingjing; Zhao, Yue; She, Ruiping; Cao, Binbin; Xiao, Peng; Wu, Qiaoxing; Guo, Zhaojie; Ma, Longhuan; Soomro, Majid Hussain

    2014-01-01

    Rabbit hepatitis E virus (HEV) is a novel genotype of HEV, and is considered to pose a risk of zoonotic transmission. Research into the systemic distribution of rabbit HEV in rabbits during different periods of infection has rarely been reported. To better understand this virus, we infected rabbits with second-passage rabbit HEV via an intraperitoneal route. After inoculation, the infection showed two types, temporary and constant infection. The detection of HEV RNA in the feces varied with time, and serum antigen correlated with fecal HEV RNA. Viremia only appeared 72 days after inoculation. The rabbits remained antibody negative throughout the experimental period. When HEV was localized, several organs besides the liver were HEV RNA positive. Tissue antigen was observed immunohistochemically in the different cells of various organs, especially in parts of the small intestine and the characteristic rabbit gut-associated lymphoid tissue. These data provide valuable information for future research into the pathogenesis of HEV.

  15. Effect of a dietary supplementation with linseed oil and selenium to growing rabbits on their productive performances, carcass traits and fresh and cooked meat quality.

    Science.gov (United States)

    Matics, Zs; Cullere, M; Szín, M; Gerencsér, Zs; Szabó, A; Fébel, H; Odermatt, M; Radnai, I; Dalle Zotte, A; Szendrő, Zs

    2017-08-01

    The present experiment tested a dietary supplementation with linseed oil and selenium to growing rabbits. The basal diet (B) contained 3% sunflower oil, while it was substituted with 3% linseed oil in the experimental feed (S). The selenium (Se) content of the two diets was 0.10 vs. 0.46 mg/kg. Rabbits were fed with B diet from the age of 18 days. One group was fed with the B diet until 11 weeks of age (group B), whereas the experimental groups were fed with S diet for 1, 2, 3 or 4 weeks (groups S1, S2, S3 and S4, respectively), before slaughtering (11 weeks of age). Live performance and carcass traits of rabbits, fatty acid (FA) profile and selenium content of their hind leg (HL) and Longissimus thoracis et lumborum (LTL) meat were considered in this study. In addition, the effect of two different cooking methods on the nutritional value of the enriched HL meat was also assessed. The tested dietary supplementation only minimally affected the live performance and carcass traits of rabbits. The S supplementation significantly reduced the Σ n-6 FA and increased the Σ n-3 FA of the HL meat and LTL meat, compared to the B diet (p cooking loss, Se and vitamin E contents as well as the oxidative status of the HL meat (p cooking methods providing different results. In addition, even if the beneficial C20:5 n-3 and C22:6 n-3 decreased with cooking, the n-6/n-3 ratio remained unaffected. Journal of Animal Physiology and Animal Nutrition © 2016 Blackwell Verlag GmbH.

  16. Crossbreeding effects on rabbit reproduction from four maternal lines of rabbits.

    Science.gov (United States)

    Ragab, M; Sánchez, J P; Mínguez, C; Baselga, M

    2016-07-01

    Litter size is essential for an efficient production of rabbit meat. A diallel cross between four maternal lines was carried out and the analysis of the components of litter size has been already done. This paper presents the analysis of litter size traits themselves (total born (TB), number born alive (NBA), number weaned (NW)) and kindling interval (KI), that complete the analysis of the reproductive performance. The 16 genetic groups were distributed in four Spanish farms. The V line was present in all farms in order to be used as reference group. A total of 34 546 parities from 7111 does, were analysed. The crossbreeding parameters were estimated according to Dickerson model. The differences between lines performance were of low magnitude and not significant for litter size traits. The LP line showed the shortest KI followed by H respect to lines A and V. These differences reflected the differences between direct and maternal genetic effects. The differences between the average of all crosses and line V were found to be significant and seemed to be important, being 0.46 for TB, 0.56 for NBA, 0.75 for NW and -2.21 days for KI. The differences between reciprocal crosses for litter size were of low magnitude and non-significant, which indicate that the maternal effects are not important between these lines. In general, the lines did not show significant differences in direct and maternal genetic effects for TB, NBA and NW but there were some significant differences for KI, which ranged from 1.54 to 6.85 days in direct effects and from 0.63 to 3.38 days for maternal effects. A positive and, in some cases, relevant heterosis was found. The largest heterosis was for TB in the HV cross (1.05 rabbits), followed by the AH (0.74 rabbits), AV (0.57 rabbits) and LH (0.55 rabbits) crosses. For NBA, significant heterosis was found in HV (1.11 rabbits) and AV (0.49 rabbits) and for NW in AV (0.90 rabbits), LH (0.70 rabbits) and LV (0.58 rabbits). Favourable and significant

  17. In-vitro effects of PDE5 inhibitor and statin treatment on the contractile responses of experimental MetS rabbit's cavernous smooth muscle

    Directory of Open Access Journals (Sweden)

    Yasin Erden

    2014-03-01

    Full Text Available Objective: Hypercholesterolaemia promotes erectile dysfunction through increased superoxide formation and decreased nitric oxide bioactivity in cavernosal tissue. The role of nitric oxide on erectile function is well known. Statins have lipid lowering properties and can modulate endothelial nitric oxide bioavailability. Sildenafil, enhances smooth muscle relaxation in corpus cavernosum. We invastigated in-vitro effects of sildenafil and rosuvastatin on nonadrenergic, non-cholinergic and nitric oxide mediated cavernosal smooth musle relaxation in metabolic syndrome rabbits, since alterations in this pathway are recognised in diabetic and hypercholesterolemic erectile dysfunction. Methods: Ten male rabbits were fed a standart diet as control group, fourty male rabbits were fed a hypercholesterolemic diet for 12 weeks. Hypercholesterolemic group were divided for without treatment, rosuvastatin treatment, sildenafil teratment, and rosuvastatin + sildenafil treatment (N = 10 per groups. Results: Serum levels of cholesterol and glucose were significantly higher in the experimental group than in the control group (p < 0.05. After theraphy no differences were found among the groups in relaxation responses to sodium nitroprusside. The relaxation responses to carbachol and EFS were significantly reduced in metabolic syndrome group to control group (p < 0.05, but there were no differences between the other groups and control group. There was a significantly lower in-vitro relaxation response in the metabolic syndrome rabbits than in controls and the others (p < 0.05. Conclusion: Both agents improve in-vitro relaxation responses of erectile tissue from metabolic syndrome rabbits to endothelial non-adrenergic, non-cholinergic and nitric oxide. This finding supports to the results of other clinical studies with these drugs.

  18. Effect of feed restriction and feeding plans on performance, slaughter traits and body composition of growing rabbits

    Directory of Open Access Journals (Sweden)

    Marco Birolo

    2017-06-01

    Full Text Available Two feeding systems (L, ad libitum vs. R, feed restriction were combined with 3 feeding plans (MM, MH, HH to evaluate the effects on performance, slaughter results, body composition and nitrogen balance of 300 commercial crossbred rabbits kept individually from weaning to slaughter (34-70 d of age. The R rabbits were fed from 80% (first days on trial to 100% (end of 3rd wk of ad libitum intake, whereafter R rabbits had free access to feed. The 3 feeding plans were: MM plan, M diet with moderate digestible energy (DE content, 10.6 MJ/kg, throughout the trial; HH plan, H diet with high DE content, 11.1 MJ/kg, throughout the trial; MH plan, M diet for the first 3 wk and H diet for the last 2 wk. Feed restriction did not affect nutrient digestibility, growth rate on the whole trial and slaughter results, but improved feed conversion (2.96 vs. 2.89 in L and R rabbits, respectively; P<0.01 and reduced N excretion (2.16 vs. 2.07 g excreted N/d, in L and R rabbits; P<0.05. At the end of the first period (55 d, R rabbits showed lower empty body protein, lipid, and gross energy gains than L rabbits, but differences disappeared within the end of the trial. The HH plan improved feed conversion (2.97 vs. 2.89 for MM vs. HH; P<0.05, but increased excreted N (2.03 vs. 2.17 g/d; P<0.001 in comparison with the MM plan due to the higher digestible protein/DE ratio of H diet, whereas the MH plan showed intermediate results. In conclusion, a moderate feed restriction during post weaning improved feed conversion and reduced N excretion without negative effects on growth or slaughter results. Moreover, N excretion was confirmed to depend largely on dietary nitrogen content.

  19. Adverse reactions in a population of Sydney pet rabbits vaccinated against rabbit calicivirus.

    Science.gov (United States)

    Tung, T; Phalen, D; Toribio, J-Alml

    2015-11-01

    To determine the general clinical presentation and incidence of adverse reactions to Cylap® RCD vaccinations, of a nature serious enough for veterinary attention, in a Sydney population of pet rabbits. A retrospective survey using hospital databases. Nine veterinary hospitals in Sydney participated in a database search for the number of rabbits vaccinated within a 2-year period. The hospitals involved had an identified interest in rabbit medicine and included general, specialist and teaching hospitals. Details of the rabbit, vaccination event and any possible reaction were collected and analysed. Of 933 events recorded in 705 rabbits, 17 (1.8%) adverse reactions were observed. Of the adverse events, local injection site reactions (alopecia, abrasions and scabbing) were most common. Other reactions, including systemic signs of gastrointestinal tract stasis, lethargy and forelimb lameness, were also documented. Overall, rabbits presented for vaccination were mostly male (57.7%) and desexed (71.3%), with an average age of 28.1 months (median 19.0, range 1.4-149.8 months) and an average weight at first vaccination of 2.12 kg (median 2.08 kg, range 0.18-5.6 kg). A significant association between increasing age and decreased incidence of adverse events was demonstrated (P value, 0.038). The benefits of vaccination against RCV outweigh the risks of an adverse reaction occurring. Data from this study show that adverse reactions occur infrequently, are generally mild and self-resolving, and decrease in incidence with increasing age. These results are similar to previous field research on wild rabbit colonies and reports from government and industry. © 2015 Australian Veterinary Association.

  20. Innate resistance to myxomatosis in wild rabbits in England.

    Science.gov (United States)

    Ross, J; Sanders, M F

    1977-12-01

    Wild rabbits (Oryctolagus cuniculus) from one study area in England have been used over a period of 11 years to investigate the possible appearance of innate resistance to myxomatosis. Rabbits of 4-6 weeks old were captured alive, retained in the laboratory until at least 4 months old, and then infected with a type of myxoma virus which kills 90-95% of laboratory rabbits. Observations were made of symptoms, mortality rate and survival times.In the first 4 years of the study (1966-9), mortality rates were not significantly different from those of laboratory rabbits, although survival times of wild rabbits were appreciably longer. In 1970, the mortality rate amongst wild rabbits was 59%, in 1974 it was 17%, and in 1976 it was 20%, thus showing that a considerable degree of inherited resistance to myxomatosis has developed.The types of myxoma virus most commonly isolated from wild rabbits in Great Britain in recent years have been those which cause 70-95% mortality in laboratory rabbits. Therefore, if the degree of innate resistance demonstrated is widespread in Great Britain, there are serious implications regarding the size of the rabbit population, because myxomatosis has been an important factor in holding rabbit numbers at a relatively low level.

  1. Innate resistance to myxomatosis in wild rabbits in England*

    Science.gov (United States)

    Ross, J.; Sanders, M. F.

    1977-01-01

    Wild rabbits (Oryctolagus cuniculus) from one study area in England have been used over a period of 11 years to investigate the possible appearance of innate resistance to myxomatosis. Rabbits of 4-6 weeks old were captured alive, retained in the laboratory until at least 4 months old, and then infected with a type of myxoma virus which kills 90-95% of laboratory rabbits. Observations were made of symptoms, mortality rate and survival times. In the first 4 years of the study (1966-9), mortality rates were not significantly different from those of laboratory rabbits, although survival times of wild rabbits were appreciably longer. In 1970, the mortality rate amongst wild rabbits was 59%, in 1974 it was 17%, and in 1976 it was 20%, thus showing that a considerable degree of inherited resistance to myxomatosis has developed. The types of myxoma virus most commonly isolated from wild rabbits in Great Britain in recent years have been those which cause 70-95% mortality in laboratory rabbits. Therefore, if the degree of innate resistance demonstrated is widespread in Great Britain, there are serious implications regarding the size of the rabbit population, because myxomatosis has been an important factor in holding rabbit numbers at a relatively low level. PMID:270526

  2. FedEX Sponsors China's Badminton Team

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    The reigning world champion team joins hands with the express delivery service to greet the coming Olympics Starting this year, Olympic badminton champion Zhang Ning and world badminton champion Lin Dan will be wearing clothing with the FedEx logo on it.

  3. Wideband electromagnetically coupled coaxial probe fed slot ...

    African Journals Online (AJOL)

    user

    ... and low gain that limits its application in practice (Bahl et al, 1980 and Balanis, ... distribution in upper and lower patches at both the resonance frequencies is .... The equivalent circuit of rectangular patch stacked with U-slot loaded fed patch ...

  4. Supervision of Fed-Batch Fermentations

    DEFF Research Database (Denmark)

    Gregersen, Lars; Jørgensen, Sten Bay

    1999-01-01

    Process faults may be detected on-line using existing measurements based upon modelling that is entirely data driven. A multivariate statistical model is developed and used for fault diagnosis of an industrial fed-batch fermentation process. Data from several (25) batches are used to develop a mo...

  5. A comparative study of antioxidant vitamins and simvastatin in hypercholesterolimic rabbits.

    Science.gov (United States)

    Syed, Nawazish-I-Husain; Arshad, Shamas; Syed, Amir Ali Rizvi; Ahmad, Maqsood; Khan, Farrakh Zia; Ahmad, Bashir

    2011-10-01

    The anti-lipidemic effects of orally administered antioxidant vitamins (vitamin A, vitamin C and vitamin E) individually and in combination were studied in cholesterol-fed rabbits and compared to the group of hypercholesterolemic animals that were treated with simvastatin. All treatment groups exhibited a decrease in serum total cholesterol, low density lipoprotein-cholesterol (LDL-C) and triglycerides concentrations, whilst vitamin C, vitamin E, the combination and simvastatin showed a more profound decrease in the lipid profile than vitamin A at different time intervals. The order of increase in high density lipoprotein-cholesterol (HDL-C) levels remained in favour of simvastatin, as none of the antioxidant vitamins treated group could exhibit a profound increase in the HDL-C.

  6. Pomegranate peel as a natural antioxidant enhanced reproductive performance and milk yield of female rabbits

    Directory of Open Access Journals (Sweden)

    H.S. Zeweil

    2016-09-01

    Full Text Available This study was designed to investigate the effects of dietary pomegranate peel (PP; 9.61% crude protein and 13.1% crude fibre on reproductive performance and milk yield of heat-stressed rabbit does. Forty-eight V-line rabbit does with average initial live body weight of 3.59±0.11 kg (2nd parity; 9 mo old were divided into 4 treatment  roups (12 does per group. The first group was fed diet free of PP. The second, third and fourth groups were fed diets containing 0.75, 1.5 and 3.0% PP, respectively, included in substitution of clover hay from the basal diet. Feed of the 4 groups was free of added antioxidants. The studied traits were: kindling rate, gestation period, litter size, litter weight, kit weight at both birth and weaning (28 d of lactation for the last 3 parameters, pre-weaning mortality and milk yield. Range of temperatures and relative humidity during the experimental period in the rabbitry varied between 27.5-33.5°C and 64-76%, respectively. The inclusion of PP quadratically affected litter size at birth (P<0.001 and weaning (P<0.01, as well as milk yield (P<0.001. However, it tended to reduce the kit milk intake in most lactation periods (P=0.078, leading to a linear reduction in individual kit weight at weaning (P=0.060. In conclusion, supplementations of PP in the diet of does during summer season in Egypt can improve their productivity.

  7. Intake of heat-expanded amaranth grain reverses endothelial dysfunction in hypercholesterolemic rabbits.

    Science.gov (United States)

    Caselato-Sousa, Valeria Maria; Ozaki, Michiko Regina; de Almeida, Eros Antonio; Amaya-Farfan, Jaime

    2014-12-01

    This study reports the new functional property of amaranth grain against diet-induced endothelial dysfunction in rabbits. Twenty-seven New Zealand rabbits were fed either a standard diet (SD/G1) or a hypercholesterolemic diet (Hichol) for 28 days. On day 29, the Hichol group was subdivided into four groups and begun receiving the following diets for 21 days: G2: SD + amaranth, G3: Hichol + amaranth, G4: SD alone, and G5: Hichol alone, while G1 continued to receive SD for 21 days. Amaranth intake restored endothelial function (G2, G3) to nearly normal during the 21-day recovery besides substantially lowering total and LDL blood cholesterol levels. This effect was not seen by simply correcting the diet (G4). Upon continuance of Hichol, however, amaranth supplementation did show some contribution to the cholesterol-lowering effect (G4 vs. G3). On day 49, feeding Hichol without the help of amaranth, harm was further magnified by lowering HDL-cholesterol (G5). Fecal cholesterol was found increased in groups that ingested amaranth (G2, G3), but no significant impact from either supplementation or diet reversal was found in fecal bile acids. Amaranth supplementation granted some protection against tissue cholesterol (G5) and tissue peroxidation (G3). It is concluded that even in concurrence with a hypercholesterolemic diet, intake of heat-expanded amaranth can revert an associated endothelial dysfunction besides incrementing fecal cholesterol excretion and lowering blood and tissue cholesterol oxidation in dyslipidemic rabbits. These results supported the notion of a lipid peroxidation process occurring with high cholesterol intakes.

  8. Influence of calorie source on the physiological response to parenteral nutrition in malnourished rabbits.

    Science.gov (United States)

    García-Domingo, M; Lladó, L; Guirao, X; Franch, G; Oliva, A; Muñoz, A; Salas, E; Sancho, J J; Sitges-Serra, A

    1994-02-01

    The present study was designed to investigate whether calorie source influences sodium and water metabolism and sympathetic activity during parenteral nutrition (PN). 20 New Zealand rabbits were starved until a mean weight loss of 18% was achieved and then re-fed for 6 days with 2 formulae of PN with different glucose-fat proportions. In the Glucose group (n = 9), 70% of non-protein calories were given as glucose while in the Lipid group (n = 11), 70% of non-protein calories were administered as lipids. Rabbits with a high glucose intake showed significantly higher weight gain (151 +/- 87 vs. 52 +/- 7 g, P = 0.01), water cumulative balance (542 +/- 132 vs. 411 +/- 87 ml; P = 0.02) and urinary metanephrine excretion (0.42 +/- 0.12 vs. 0.30 +/- 0.1 mumol/d, P = .03). Only in this group, urinary metanephrines correlated positively with water and sodium balances (r2 = 0.6; P = 0.02 and r2 = 0.7; P = 0.009 respectively). The Glucose group showed 2 different responses and in a second experiment 10 additional rabbits were added to this group to allow a statistical analysis of the response pattern: half of the animals increased their extracellular water (ECW) compartment while the remaining animals did not. The former group had higher sodium balance (13.9 +/- 8 vs. 4.3 +/- 5; P = 0.004) and wet lung weight (8.9 +/- 0.9 vs. 7.9 +/- 0.8; P wate and sodium balances. Plasma aldosterone concentration at the end of starvation period influences sodium retention and ECW expansion during high glucose re-feeding.

  9. Myosin light chain kinase inhibitor ML7 improves vascular endothelial dysfunction via tight junction regulation in a rabbit model of atherosclerosis.

    Science.gov (United States)

    Cheng, Xiaowen; Wang, Xiaobian; Wan, Yufeng; Zhou, Qing; Zhu, Huaqing; Wang, Yuan

    2015-09-01

    Vascular endothelial dysfunction (VED) is an important factor in the initiation and development of atherosclerosis (AS). Previous studies have demonstrated that endothelial permeability is increased in diet‑induced AS. However, the precise underlying mechanisms remain poorly understood. The present study aimed to analyze whether the myosin light chain kinase (MLCK) inhibitor ML7 is able to improve VED and AS by regulating the expression of the tight junction (TJ) proteins zona occludens (ZO)‑1 and occludin via mechanisms involving MLCK and MLC phosphorylation in high‑fat diet‑fed rabbits. New Zealand white rabbits were randomly divided into three groups: Control group, AS group and ML7 group. The rabbits were fed a standard diet (control group), a high‑fat diet (AS group) or a high‑fat diet supplemented with 1 mg/kg/day ML7 (ML7 group). After 12 weeks, endothelium‑dependent relaxation and endothelium‑independent relaxation were measured using high-frequency ultrasound. Administration of a high‑fat diet significantly increased the levels of serum lipids and inflammatory markers in the rabbits in the AS group, as compared with those in the rabbits in the control group. Furthermore, a high‑fat diet contributed to the formation of a typical atherosclerotic plaque, as well as an increase in endothelial permeability and VED. These symptoms of AS were significantly improved following treatment with ML7, as demonstrated in the ML7 group. Hematoxylin & eosin and immunohistochemical staining indicated that ML7 was able to decrease the expression of MLCK and MLC phosphorylation in the arterial wall of rabbits fed a high‑fat diet. A similar change was observed for the TJ proteins ZO‑1 and occludin. In addition, western blot analysis demonstrated that ML7 increased the expression levels of occludin in the precipitate, but reduced its expression in the supernatant of lysed aortas. These results indicated that occludin, which is a dynamic protein at the TJ

  10. MEAT QUALITY OF LOCAL AND HYBRID RABBITS

    Directory of Open Access Journals (Sweden)

    G. Paci

    2012-08-01

    Full Text Available pH, colour and oxidative status were evaluated to study the effect of rabbit genotype on meat quality. Commercial Hybrids, selected for high growth rate and a local population, characterized by slow growing, were used. Meat quality characteristics of L. lumborum and B. femoris muscles showed significant differences between genotypes. Local population had higher pHu values but lower pH fall values than Hybrids. Hybrids showed higher lightness values and TBARS contents than local population. Meat quality parameters were influenced by genotype. The differences between genotypes could be related to the different degree of maturity because the rabbits, in relation to the different growth rate, were slaughtered at the same weight but at different age.

  11. Rabbit lung injury induced by explosive decompression

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Objective: To study the mechanism of rabbit lunginjury caused by explosive decompression. Methods: A total of 42 rabbits and 10 rats were served as the experimental animals. A slow recompressiondecompression test and an explosive decompression test were applied to the animals, respectively. And the effects of the given tests on the animals were discussed. Results: The slow recompression-decompression did not cause an obvious lung injury, but the explosive decompression did cause lung injuries in different degrees. The greater the decompression range was, the shorter the decompression duration was, and the heavier the lung injuries were. Conclusions: Explosive decompression can cause a similar lung injury as shock wave does. The primary mechanical causes of the lung injury might be a tensile strain or stress in the alveolar wall and the pulmonary surface's impacts on the inside wall of the chest.

  12. Quantitative profiling of oxylipins in plasma and atherosclerotic plaques of hypercholesterolemic rabbits.

    Science.gov (United States)

    Bojic, Lazar A; McLaren, David G; Harms, Amy C; Hankemeier, Thomas; Dane, Adrie; Wang, Sheng-Ping; Rosa, Ray; Previs, Stephen F; Johns, Douglas G; Castro-Perez, Jose M

    2016-01-01

    Oxylipins are oxidation products of polyunsaturated fatty acids (PUFAs) that affect a broad range of physiological processes, including cell proliferation, inflammation, inflammation resolution, and vascular function. Moreover, oxylipins are readily detectable in plasma, and certain subsets of oxylipins have been detected in human atherosclerotic lesions. Taken together, we set out to produce a detailed quantitative assessment of plasma and plaque oxylipins in a widely used model of atherosclerosis, to identify potential biomarkers of disease progression. We administered regular chow or regular chow supplemented with 0.5% cholesterol (HC) to male New Zealand white rabbits for 12 weeks to induce hypercholesterolemia and atherosclerosis. Our targeted lipidomic analyses of oxylipins on plaques isolated from rabbits fed the HC diet detected 34 oxylipins, 28 of which were in compliance with our previously established quality control acceptance criteria. The arachidonic acid (AA) metabolite derived from the COX pathway, 6-keto-PGF1α was the most abundant plaque oxylipin, followed by the linoleic acid (LA) metabolites 9-HODE, 13-HODE and 9,12,13-TriHOME and the arachidonic acid (AA)-derivatives 11-HETE and 12-HETE. We additionally found that the most abundant oxylipins in plasma were three of the five most abundant oxylipins in plaque, namely 11-HETE, 13-HODE, and 9-HODE. The studies reported here make the first step towards a comprehensive characterization of oxylipins as potentially translatable biomarkers of atherosclerosis.

  13. Uterine metabolism of the pregnant rabbit under chronic steady-state conditions

    Energy Technology Data Exchange (ETDEWEB)

    Johnson, R.L.; Gilbert, M.; Block, S.M.; Battaglia, F.C.

    1986-05-01

    The study of uterine metabolism in pregnancy under chronic steady-state conditions has been confined to large mammals and, more recently, to the guinea pig. The pregnant rabbit is of interest because of its short gestation and large litter size. We developed an indirect approach involving retrograde catheterization of the uterine venous drainage, permitting measurement of both uterine metabolic quotients and uterine uptakes. Radioactive microspheres were used to measure blood flow. A large lactate and ammonia efflux from the uterus was found. In the fed state, ketogenic substrates were taken up in small amounts. However, during starvation a significant increase in ketoacid uptake was observed with a concurrent fall in acetate uptake. There was a large glucose/oxygen quotient across the uterus, but the glucose plus lactate/oxygen quotient was comparable to that found in the sheep and guinea pig (0.6 +/- 0.1). It is apparent that in all three species studied under chronic steady-state conditions (sheep, guinea pig, and rabbit) there is a large glucose uptake associated with a net lactate production, and fuels other than glucose and lactate must be used by the uterus.

  14. Hypocholesterolemic and Antiatherosclerotic Potential of Basella alba Leaf Extract in Hypercholesterolemia-Induced Rabbits

    Directory of Open Access Journals (Sweden)

    Gunasekaran Baskaran

    2015-01-01

    Full Text Available Hypercholesterolemia is the major risk factor that leads to atherosclerosis. Nowadays, alternative treatment using medicinal plants gained much attention since the usage of statins leads to adverse health effects, especially liver and muscle toxicity. This study was designed to investigate the hypocholesterolemic and antiatherosclerotic effects of Basella alba (B. alba using hypercholesterolemia-induced rabbits. Twenty New Zealand white rabbits were divided into 5 groups and fed with varying diets: normal diet, 2% high cholesterol diet (HCD, 2% HCD + 10 mg/kg simvastatin, 2% HCD + 100 mg/kg B. alba extract, and 2% HCD + 200 mg/kg B. alba extract, respectively. The treatment with B. alba extract significantly lowered the levels of total cholesterol, LDL, and triglycerides and increased HDL and antioxidant enzymes (SOD and GPx levels. The elevated levels of liver enzymes (AST and ALT and creatine kinase were noted in hypercholesterolemic and statin treated groups indicating liver and muscle injuries. Treatment with B. alba extract also significantly suppressed the aortic plaque formation and reduced the intima: media ratio as observed in simvastatin-treated group. This is the first in vivo study on B. alba that suggests its potential as an alternative therapeutic agent for hypercholesterolemia and atherosclerosis.

  15. Sex Difference in the Repolarization Currents of Rabbit Ventricular Cells

    Institute of Scientific and Technical Information of China (English)

    RUAN Yanfei; LIU Nian; ZHOU Qiang; LI Yang; WANG Lin

    2005-01-01

    Summary: The current difference between male and female rabbit ventricular myocytes was investigated for elucidating the mechanism of longer QT interval and higher incidence of drug-associated torsade de pointes in female rabbits than in male rabbits. Whole cell patch clamp technique was used to record APD, Ito, IK,tail, IK1 and ICa,L of myocytes from left ventricular apex. There was no difference in the membrane capacitance between male and female rabbit myocytes. APD90 was longer in female rabbits (560.4±26.5 ms, n=15) than in male ones (489.0±20.7 ms, n=14), P0.05). The lower IK,tail of female rabbit myocytes may contribute to the longer repolarization and the higher incidence of drug-associated torsade de pointes.

  16. Assessment of gastrointestinal pH, fluid and lymphoid tissue in the guinea pig, rabbit and pig, and implications for their use in drug development.

    Science.gov (United States)

    Merchant, Hamid A; McConnell, Emma L; Liu, Fang; Ramaswamy, Chandrasekaran; Kulkarni, Rucha P; Basit, Abdul W; Murdan, Sudaxshina

    2011-01-18

    Laboratory animals are often used in drug delivery and research. However, basic information about their gastrointestinal pH, fluid volume, and lymphoid tissue is not completely known. We have investigated these post-mortem in healthy guinea pigs, rabbits and pigs, to assess their suitability for pre-clinical studies by comparing the results with reported human literature. The mean gastric pH (fed ad libitum) was 2.9 and 4.4 in guinea pig and pig, respectively. In contrast, a very low pH (1.6) was recorded in the rabbits. The small intestinal pH was found in the range of 6.4-7.4 in the guinea pigs and rabbits, whereas lower pH (6.1-6.7) was recorded in the pig, which may have consequences for ionisable or pH responsive systems when tested in pig. A relatively lower pH than in the small intestine was found in the caecum (6.0-6.4) and colon (6.1-6.6) of the guinea pig, rabbit and the pig. The water content in the gastrointestinal tract of guinea pig, rabbit and pig was 51g, 153g and 1546g, respectively. When normalized to the body weight, the guinea pig, had larger amounts of water compared to the rabbit and the pig (guinea pig>rabbit>pig); in contrast, a reverse order was found when normalized to per unit length of the gut (guinea pigpig). The lymphoid tissue distribution (lymphoid follicles, Peyer's patches and long strips) along the length of the gut in these animals is presented; in particular, an abundance of lymphoid tissue was found in pig's stomach, small intestine and caecum, and rabbit's appendix. Their ample presence indicated the potential utility of these animal species in oral and colonic vaccination. These differences in the gastrointestinal parameters of the guinea pig, rabbit and pig reiterates the crucial importance of correctly selecting animal models for pre-clinical studies.

  17. The Effect of Dietary Linseed Oil and Organic Selenium on Growth Performance and Muscle Fatty Acids in Growing Rabbits

    Directory of Open Access Journals (Sweden)

    Ahmed A. Saleh, Tarek A. Ebeid and Yahya Z. Eid*

    2013-11-01

    Full Text Available The present study was conducted to evaluate the effect of a combined in-feed of linseed oil and organic selenium on growth performance and muscle fatty acid profiles in growing rabbits. A total of 4-week-old sixty male growing New Zealand white rabbits (average weight 531±5g were collected and equally divided into 3 groups. The control group was fed on a control diet, whereas the treatment groups were fed on diets contained 2.5% linseed oil with or without 0.3 ppm organic selenium. All experimental treatments were provided from 4 to 10 weeks of age. Although feed intake was decreased significantly (P<0.05 by the dietary linseed oil and organic selenium, body weight gain was significantly (P<0.05 increased. Both plasma and muscle total cholesterol decreased with the decrease of abdominal fat. However, plasma concentrations of HDL-cholesterol and glutathione peroxidase were increased significantly (P<0.05 by dietary supplementation of linseed oil and organic selenium. Furthermore, in the muscles, saturated fatty acids were decreased; meanwhile, unsaturated fatty acids were increased that may refer to the use of linseed oil and organic selenium. In conclusion, the present study clearly shows that growth performance was improved. Muscle lipid profile could be modified by a combined in-feed of linseed oil and organic selenium to the growing rabbit’s diet.

  18. l-Citrulline and l-arginine supplementation retards the progression of high-cholesterol-diet-induced atherosclerosis in rabbits.

    Science.gov (United States)

    Hayashi, Toshio; Juliet, Packiasamy A R; Matsui-Hirai, Hisako; Miyazaki, Asaka; Fukatsu, Akiko; Funami, Jun; Iguchi, Akihisa; Ignarro, Louis J

    2005-09-20

    The objective of this study was to evaluate the influence of ingested l-arginine, l-citrulline, and antioxidants (vitamins C and E) on the progression of atherosclerosis in rabbits fed a high-cholesterol diet. The fatty diet caused a marked impairment of endothelium-dependent vasorelaxation in isolated thoracic aorta and blood flow in rabbit ear artery in vivo, the development of atheromatous lesions and increased superoxide anion production in thoracic aorta, and increased oxidation-sensitive gene expression [Elk-1 and phosphorylated cAMP response element-binding protein]. Rabbits were treated orally for 12 weeks with l-arginine, l-citrulline, and/or antioxidants. l-arginine plus l-citrulline, either alone or in combination with antioxidants, caused a marked improvement in endothelium-dependent vasorelaxation and blood flow, dramatic regression in atheromatous lesions, and decrease in superoxide production and oxidation-sensitive gene expression. These therapeutic effects were associated with concomitant increases in aortic endothelial NO synthase expression and plasma NO(2)(-)+NO(3)(-) and cGMP levels. These observations indicate that ingestion of certain NO-boosting substances, including l-arginine, l-citrulline, and antioxidants, can abrogate the state of oxidative stress and reverse the progression of atherosclerosis. This approach may have clinical utility in the treatment of atherosclerosis in humans.

  19. Dynamic and ultrastructural study of sphincter of Oddi in early-stage cholelithiasis in rabbits with hypercholesterolemia

    Institute of Scientific and Technical Information of China (English)

    Jing Guo Wei; Yao Cheng Wang; Fan Du; Hou Jun Yu

    2000-01-01

    AIM To study the relationship between preformation of gallstone and the kinetics and ultrastructure of sphincter of Oddi.METHODS Adult female rabbits were used and divided into 3 groups, and fed with either normal or high cholesterol diet for four or eight weeks.Each group contained eight rabbits. The manometry of sphincter of Oddi, biliary cineradiography, gallbladder volume measurement and ultrastructure observation under electron microscope were performed.RESULTS In groups Ⅰ and Ⅱ, the basal pressure in low-pressure ampulla or high pressure zone of sphincter of Oddi was elevated, the amplitude of phasic contraction was decreased and the volume of gallbladder were increased, with a significant difference (P<0.01) from those of control. Gallstones were found in group Ⅱ rabbits (7/8). Under cineradiography, low-pressure ampulla showed a spasmodic status without apparent peristaltic contraction. Under electron microscope, inside the muscular cells of sphincter of Oddi,loosening of microfilament and swelling of plasmosomes which congregated at the top were observed. The amount showed no obvious change under nitric oxide synthase (NOS) stain.CONCLUSION Twisting of the microfilament and disarrangement of kink macula densa inside the muscular cells suggested that the sphincter of Oddi was under spasmodic status. The impaired diastolic function caused and aggravated the stasis of cystic bile. The swelling plasmosome could be one of the important factors in elevating the tonic pressure of sphincter of Oddi.

  20. Habitat Suitability Index Models: Swamp rabbit

    Science.gov (United States)

    Allen, Arthur W.

    1985-01-01

    A review and synthesis of existing information were used to develop a Habitat Suitability Index (HSI) model for the swamp rabbit (Sylvilagus aquaticus). The model consolidates habitat use information into a framework appropriate for field application, and is scaled to produce an index between 0.0 (unsuitable habitat) to 1.0 (optimum habitat). HSI models are designed to be used with Habitat Evaluation Procedures previously developed by the U.S. Fish and Wildlife Service.

  1. Control of oxyuriasis in rabbits by fenbendazole.

    Science.gov (United States)

    Düwel, D; Brech, K

    1981-04-01

    Death, poor condition and unsatisfactory breeding performance in a rabbit colony was attributed to infection with Passalurus ambiguus. The trouble disappeared after treatment with 50 ppm fenbendazole in the food for 5 days. In laboratory trials 12.5 ppm for 5 days eliminated more than 99% of adult and most immature pinworms, 25 and 50 ppm eliminated all immature and adult pinworms. These doses were also effective against Obeliscoides cuniculi.

  2. Higher lipid accumulation in broilers fed on saturated fats than in those fed on unsaturated fats.

    Science.gov (United States)

    Sanz, M; Flores, A; De Ayala, P P; Lopez-Bote, C J

    1999-03-01

    1. Two experiments were conducted to assess the effect of fat sources differing in degree of saturation on the performance of and fat deposition in broiler chickens fed on isocaloric and isonitrogenous diets. 2. There were no differences in initial body weight between sexes but female broilers had lower daily gains (Psaturation. Broilers fed on diets containing an animal fat blend or tallow had higher abdominal fat pad weight (Psaturation affects fat accumulation in broiler chickens.

  3. Investigation of the Protective Effects of Taurine against Alloxan-Induced Diabetic Retinal Changes via Electroretinogram and Retinal Histology with New Zealand White Rabbits

    Directory of Open Access Journals (Sweden)

    Samuel Tung-Hsing Chiang

    2014-01-01

    Full Text Available The purpose of this study was to investigate the protective role of orally administered taurine against diabetic retinal changes via electroretinogram (ERG and retinal histology on rabbits. Rabbits were randomly assigned into groups: Group I (vehicle administration only; Group II (diabetes: induced by 100 mg/kg alloxan injection; Group III (diabetes and fed with 200 mg/kg taurine; and Group IV (diabetes and fed with 400 mg/kg taurine. The body weight and blood glucose levels of the rabbits were monitored weekly. The ERG was measured on weeks 5 and 15. Retinal histology was analyzed in the end of the experiment. Results revealed that a taurine supplement significantly ameliorates the alloxan-induced hyperglycemia and protects the retina from electrophysiological changes. Group II showed a significant (P0.05 between all groups and when compared with those of Group I. Our study provides solid evidences that taurine possesses an antidiabetic activity, reduced loss of body weight, and less electrophysiological changes of the diabetic retina.

  4. The stimulatory effect of LXRalpha is blocked by SHP despite the presence of a LXRalpha binding site in the rabbit CYP7A1 promoter.

    Science.gov (United States)

    Shang, Quan; Pan, Luxing; Saumoy, Monica; Chiang, John Y L; Tint, G Stephen; Salen, Gerald; Xu, Guorong

    2006-05-01

    The transcription of the cholesterol 7alpha-hydroxylase gene (CYP7A1) is greatly decreased in cholesterol-fed rabbits. To determine whether the molecular structure of the promoter is responsible for this downregulation, we cloned the rabbit CYP7A1 promoter, identified the binding sites for alpha-fetoprotein transcription factor (FTF) and liver X receptor (LXRalpha), and studied the effects of FTF, LXRalpha, and SHP on its transcription. Adding LXRalpha/retinoid X receptor together with their ligands (L/R) to the promoter/reporter construct transfected into HepG2 cells greatly increased its activity. FTF did not increase promoter activity, nor did it enhance the stimulatory effect of L/R. Mutating the FTF binding site abolished the promoter baseline activity. Increasing amounts of SHP abolished the effect of L/R, and FTF enhanced the ability of SHP to decrease promoter activity below baseline levels. Thus, downregulation of CYP7A1 in cholesterol-fed rabbits is attributable secondarily to the activation of farnesoid X receptor, which increases SHP expression to override the positive effects of LXRalpha. Although FTF is a competent factor for maintaining baseline activity, it does not further enhance and may suppress CYP7A1 transcription.

  5. Growth Performance, Meat Quality and Fatty Acid Metabolism Response of Growing Meat Rabbits to Dietary Linoleic Acid

    Directory of Open Access Journals (Sweden)

    R. G. Li

    2012-08-01

    Full Text Available An experiment was conducted to determine the effects of different amounts of dietary linoleic acid (LA on growth performance, serum biochemical traits, meat quality, fatty acids composition of muscle and liver, acetyl-CoA carboxylase (ACC and carnitine palmitoyl transferase 1 (CPT 1 mRNA expression in the liver of 9 wks old to 13 wks old growing meat rabbits. One hundred and fifty 9 wks old meat rabbits were allocated to individual cages and randomly divided into five groups. Animals in each group were fed with a diet with the following LA addition concentrations: 0, 3, 6, 9 and 12 g/kg diet (as-fed basis and LA concentrations were 0.84, 1.21, 1.34, 1.61 and 1.80% in the diet, respectively. The results showed as follows: the dietary LA levels significantly affected muscle color of LL included a* and b* of experimental rabbits (p<0.05. The linear effect of LA on serum high density lipoprotein cholesterol was obtained (p = 0.0119. The saturated fatty acids (SFAs and monounsaturated fatty acids (MUFAs contents of LL decreased and the polyunsaturated fatty acids (PUFAs content of LL increased with dietary LA increase (p<0.0001. The PUFA n-6 content and PUFA n-3 content in the LL was significantly affected by the dietary LA levels (p<0.01, p<0.05. The MUFAs content in the liver decreased and the PUFAs contents in the liver increased with dietary LA increase (p<0.0001. The PUFA n-6 content and the PUFA n-6/n-3 ratio in the liver increased and PUFA n-3 content in the liver decreased with dietary LA increase (p<0.01. The linear effect of LA on CPT 1 mRNA expression in the liver was obtained (p = 0.0081. In summary, dietary LA addition had significant effects on liver and muscle fatty acid composition (increased PUFAs of 9 wks old to 13 wks old growing meat rabbits, but had little effects on growth performance, meat physical traits and mRNA expression of liver relative enzyme of experimental rabbits.

  6. Atherosclerosis lesion is accelerated by persistent systemic inflammation but attenuated by saponins from Panax Notoginseng in rabbits

    Institute of Scientific and Technical Information of China (English)

    Jia Yi; Li Xiaohui; Liu Ya; Zhang Haigang

    2008-01-01

    Objective: To explore the roles of persistent systemic inflammation in atherosclerosis and the effects of saponins of Panax Notoginseng (PNS) on this process in rabbits. Methods: Thirty rabbits were divided randomly and equally into 6 groups, i.e., control, high-fat diet, inflammation, aspirin, PNS and simple-inflammation group. All the animals except that in control group and simple-inflammation group were fed with high-fat diet for 8 weeks. Based on that,rabbits in inflammation, aspirin and PNS groups were treated with zymosan injection (10 mg/kg, i.p.). Normal saline was given to rabbits in control group. Besides zymosan injection, animals in aspirin and PNS group were administrated with aspirin (12 mg/kg, i.g.) and PNS (120 mg/kg, i.g.) respectively. The animals in simple-inflammation group were treated with zymosan injection (10mg/kg, i.p.) and fed with normal diet. The atherosclerosis lesion in aortas was observed by Sudan IV staining. Serum total cholesterol, triglyceride (TG), TNF-α and activity of post-heparin lipoprotein lipase (LPL) were measured at the end of the 4th and 8th week after an overnight fast. Results: Compared with high-fat diet group, the area of atherosclerosis lesion, serum TG and TNF-α were markedly increased in rabbits of inflammation group, and the activity of LPL was decreased remarkably. Serum TNF-α level was negatively correlated with the activity of post-heparin LPL (r=-0.708, P<0.01). The area of atherosclerosis, serum TG and TNF-α were decreased in aspirin and PNS group compared with that in inflammation group, and the activity of LPL was increased remarkably. Compared with control group, serum TG and TNF-α were markedly increased in simple-inflammation group, while LPL activity was decreased. Atherosclerotic lesion did not occur in simple-inflammation group. Conclusion: Persistent systemic inflammation could accelerate the formation of atherosclerosis lesion in aortas, which partly depend on the decreasing of the activity

  7. The rabbit pup, a natural model of nursing anticipatory activity

    OpenAIRE

    Caba, Mario; González-Mariscal, Gabriela

    2009-01-01

    Mother rabbits nurse their young once a day with circadian periodicity. Nursing bouts are brief (ca. 3 min) and occur inside the maternal burrow. Despite this limited contact mother rabbits and their pups are tuned to each other to ensure that the capacities of each party are used efficiently to ensure the weaning of a healthy litter. In this review we present behavioral, hormonal, metabolic and hormonal correlates of this phenomenon in mother rabbits and their pups. Research is revealing tha...

  8. Medetomidine--ketamine--diazepam anesthesia in the rabbit.

    Science.gov (United States)

    Mero, M; Vainionpää, S; Vasenius, J; Vihtonen, K; Rokkanen, P

    1989-01-01

    Orthopaedic operations were performed in 340 rabbits when developing biodegradable rods for the fixation of fractures. The rabbits were anaesthesized by injecting medetomidine combined with ketamine and diazepam (MKD) subcutaneously. The mortality due to the anaesthesia was zero. Arterial blood gas analyses showed a moderate decrease in the haemoglobin oxygen saturation and compensated respiratory acidosis, which was well tolerated by the animals. It is concluded that MKD anaesthesia is well suited for orthopaedic operations in the rabbit.

  9. Transgenic rabbit that expresses a functional human lipoprotein (a)

    Science.gov (United States)

    Rouy, Didier; Duverger, Nicolas; Emmanuel, Florence; Denefle, Patrice; Houdebine, Louis-Marie; Viglietta, Celine; Rubin, Edward M.; Hughes, Steven D.

    2003-01-01

    A transgenic rabbit which has in its genomic DNA sequences that encode apolipoprotein (a) and apolipoprotein B polypeptides which are capable of combining to produce lipoprotein (a), a process for creating such a rabbit, and the use of the rabbit to identify compounds which are effective in the treatment of human diseases which are associated with, induced and/or exacerbated by Lp(a) expression.

  10. Myxomatosis in farmland rabbit populations in England and Wales.

    OpenAIRE

    Ross, J; Tittensor, A. M.; Fox, A. P.; Sanders, M. F.

    1989-01-01

    The overall pattern and consequences of myxomatosis in wild rabbit populations were studied at three farmland sites in lowland southern England and upland central Wales between 1971 and 1978. When results from all years were combined, the disease showed a clear two-peaked annual cycle, with a main autumn peak between August and January, and a subsidiary spring peak during February to April. Rabbit fleas, the main vectors of myxomatosis in Britain, were present on full-grown rabbits in suffici...

  11. Exogenous Streptococcus pneumoniae Endophthalmitis in Diabetic Rabbits

    Science.gov (United States)

    Benton, Angela H.; Fulton, Linda K.; Marquart, Mary E.

    2017-01-01

    Diabetics are at increased risk for eye infections including bacterial endophthalmitis. It is unclear whether the severity of endophthalmitis is greater in these patients due to confounding factors such as pre-existing ocular diseases in some but not others. Therefore, we tested the hypothesis that disease severity and/or bacterial loads would be significantly higher in a Type I diabetic rabbit model of Streptococcus pneumoniae endophthalmitis. Rabbits were treated with alloxan to destroy pancreatic islet cells, or mock-treated with vehicle, and maintained for 10 days before intravitreal infection with S. pneumoniae E353. Clinical scoring of the eyes was performed 24 and 48 hours after infection, followed by euthanasia and vitreous harvest to quantitate bacterial loads. There were no significant differences in clinical scores (P ≥ 0.440) or bacterial loads (P = 0.736), however, 4/12 (33%) of the diabetic rabbits became bacteremic. This finding not only indicates a breakdown in the blood-ocular barrier, but also prompts further investigation into the exploitation of the diabetic eye by the streptococci. PMID:28387365

  12. Reproductive activity and welfare of rabbit does

    Directory of Open Access Journals (Sweden)

    C. Castellini

    2010-04-01

    Full Text Available This paper reviews the relationships between reproductive performance and welfare of the rabbit does. In the last 10 years the profitability of rabbit farms has increased mainly due to improvements in management and genetic selection but several problems mainly related to animal welfare have also occurred. The mortality and rates of female replacement per year are very high and the replaced females often show poor body condition and low performance. The effect of kindling order, litter size, genetic strain, weaning age and reproduction rhythm on the reproductive performance and welfare of females and some mechanisms implicated in these effects are discussed. Modern rabbit does produce a lot of milk which have a high energetic value which leads to a mobilization of body fat which results in an energy deficit. In the current reproductive rhythms, there is an extensive overlap between lactation and gestation. The resulting energetic and hormonal antagonism reduces the fertility rate and lifespan of the doe. Strategies to improve the fertility, lifespan and welfare of does are discussed. An approach which combines various strategies seems to be required to meet these objectives. Since the factors involved in this productive system are fixed (genetic strain, environment the most powerful way to improve doe welfare is to choose a reproductive rhythm that is adapted to the physiology of the does.

  13. Intravitreal memantine retinal toxicity in rabbits.

    Science.gov (United States)

    Moreno Páramo, D; Reyna Vielma, S; Rodríguez Reyes, A; Hernández Ayuso, I; Quiroz Mercado, H

    2016-02-01

    To histologically evaluate whether the intravitreal application of memantine produces retinal toxicity in rabbits. A cross-sectional design, experimental, descriptive study was performed on 16 eyes of 16 New Zealand rabbits of 3 kg, divided in 4 groups of 4 rabbits. A dose of 70 ng/ml of intravitreal memantine was administered in Group A, a dose of 150 ng/ml in Group B, a dose of 400 ng/ml in Group C, and Group D received 1 ml of balanced salt solution. The injected eye of half of each group was enucleated 15 days after the injection, and the rest within 30 days after injection. Following enucleation, each eye was placed in 10% formaldehyde. Histopathological analysis was performed on all enucleated eyes. The animals were treated according to the guidelines of the Association for Research on Vision and Ophthalmology (ARVO). Groups A, B and D did not show any histopathological changes after their enucleation at 15 and 30 days. Group C showed changes in the photoreceptor layer after enucleation at 15 and 30 days. In our study, it was observed that memantine concentrations at 70 ng/ml and 150 ng/ml are safe when administered intravitreally; however, doses of 400 ng/ml produced retinal structural changes. This research should continue to assess its clinical usefulness. Copyright © 2015 Sociedad Española de Oftalmología. Published by Elsevier España, S.L.U. All rights reserved.

  14. Sodium nitroprusside induces apoptosis of rabbit chondrocytes

    Science.gov (United States)

    Liang, Qian; Wang, Xiao-Ping; Chen, Tong-Sheng

    2013-02-01

    Osteoarthritis (OA) is characterized by a slowly progressing degradation of the matrix and destruction of articular cartilage. Apoptosis of chondrocyte is accounted for the mechanism of OA. Nitric oxide (NO), as a stimulus, has been shown to induce chondrocyte apoptosis by activating the matrix metalloproteinases (MMPs), increasing the expression of cyclooxygenase 2 (COX-2) and the level of prostaglandin E2 (PGE2), inhibiting the proteoglycan synthesis and type II collagen expression. In this study, sodium nitroprusside (SNP) was administered to be the NO donor to explore the mechanism of NO-induced apoptosis of rabbit chondrocytes obtained from six weeks old New Zealand rabbits. CCK-8 assay revealed the inhibitory effect of SNP on cell viability. We used flow cytometry (FCM) to assess the form of cell death by Annexin-V/propidium iodide (PI) double staining, and evaluate the change of mitochondrial membrane potential (ΔΨm). We found that the SNP induced chondrocyte apoptosis in a dose- and time-dependent manner and an observable reduction of ΔΨm. In conclusion, our findings indicate that SNP induces apoptosis of rabbit chondrocytes via a mitochondria-mediated pathway.

  15. Nerve growth factor enhances sleep in rabbits.

    Science.gov (United States)

    Takahashi, S; Krueger, J M

    1999-04-02

    Nerve growth factor (NGF) elicits rapid-eye-movement sleep (REMS) in cats. Removal of NGF receptor-positive cholinergic basal forebrain neurons inhibits REMS in rats. The aim of the present study was to determine the effects of NGF on sleep and brain temperature (Tbr) in rabbits. Male rabbits were implanted with electroencephalograph (EEG) electrodes, a brain thermistor and an intraventricular (i.c.v.) guide cannula. Rabbits received human beta-NGF i.c.v. (0.01, 0.1, 1.0 or 10 microg] and on a separate day, 25 microl pyrogen-free saline i.c.v. as control. EEG and Tbr were recorded for 23 h after injections. The highest two doses of NGF increased both non-REMS and REMS across the 23-h recording period. REMS was enhanced dose-dependently. Tbr was not affected by any dose of NGF. These results suggest that NGF is involved in both REMS and non-REMS regulation.

  16. Effects of replacing palm oil with maize oil and Curcuma longa supplementation on the performance, carcass characteristics, meat quality and fatty acid profile of the perirenal fat and muscle of growing rabbits.

    Science.gov (United States)

    Peiretti, P G; Masoero, G; Meineri, G

    2011-04-01

    An experiment has been conducted to study the effects of the inclusion of plant oil in rabbit diets. This study was aimed at evaluating the beneficial effects of the inclusion of maize oil (MO), rich in unsaturated fatty acids (UFAs), compared to palm oil (PO) containing saturated fatty acids (SFAs), on the meat fatty acid (FA) profile. As UFAs are susceptible to rancidity, Curcuma longa (CL), which is known for its antioxidant properties, was also added (3 g/kg) to the diet with two plant oils. CL contains curcuminoids, volatile oils, sugars, proteins, resins and polyunsaturated fatty acids (PUFAs). We also evaluated the influence of CL inclusion in the diet on the FA profile of the meat. Furthermore, the possibility of using these oil-enriched diets and the ability to assimilate CL in rabbits was evaluated by analysing the performance, carcass characteristics and meat quality. At the end of the experiment, there were no significant differences between the groups concerning the live weight, live weight gain, feed consumption, feed efficiency, carcass yield or the percentages of edible organs. The hind legs, forelegs, loins and abdominal wall, breast and ribs, skin and limbs and head were not affected by the oil type or by the inclusion of CL. The chemical composition, pH and oxidative status of the Longissimus dorsi muscle of the rabbits fed the experimental diets were not affected by the oil source or by the CL supplementation. Conversely, it has been shown that it is possible to modify the FA profile of rabbit meat and fat by dietary means. The SFA/PUFA ratio significantly decreased from -18% to -16% in the meat and from -25% to -23% in the perirenal fat of the rabbits fed diets containing MO without or with CL supplementation, respectively, compared to same tissues of the rabbits fed diets containing PO without or with CL supplementation, respectively. Similar trends were found for the atherogenic index, which decreased from -20% to -17% in the meat and from

  17. Assessment of a Novel Vaccine Against Rabbit Hemorrhagic Disease Virus in Young Rabbits.

    Science.gov (United States)

    Pacho, Sonsoles; Dahdouh, Elias; Merino, Javier; Suárez, Monica

    2016-12-01

    Rabbit hemorrhagic disease virus (RHDVb) is the new variant of the classical RHDV, a virulent pathogen responsible for an acute disease in young rabbits. The virus invades internal organs, especially the liver, spleen, kidneys, and gut; prevents coagulation; and causes liver necrosis. This eventually leads to quick death of the animal because of hemorrhage. In this study, we evaluated the effects of a new vaccine against RHDVb in rabbits at a young age, after experimental infection using four different viral isolates. Our findings show that the vaccine had a protective effect with survival rates reaching 80-100% against the different isolates. These results suggest that this vaccine, when applied to young animals, is an effective tool to protect against the disease caused by RHDVb in rabbitries.

  18. An Annotated Bibliography on the Rabbit Novels by John Updike

    OpenAIRE

    2011-01-01

    Se presenta un registro comentado de los principales estudios, libros y artículos sobre la serie narrativa del novelista estadounidense John Updike, conocida bajo el título común de novelas sobre Conejo (Corre, Conejo; El regreso de Conejo; Conejo es rico, etc.).This annotated bibliography includes a brief introduction to the Rabbit novels and comments on the main studies, books and articles on these novels by the U.S. writer John Updike (Rabbit, Run; The Redux of Rabbit; Rabbit Is Rich, etc....

  19. Human handling promotes compliant behavior in adult laboratory rabbits.

    Science.gov (United States)

    Swennes, Alton G; Alworth, Leanne C; Harvey, Stephen B; Jones, Carolyn A; King, Christopher S; Crowell-Davis, Sharon L

    2011-01-01

    Routine laboratory procedures can be stressful for laboratory animals. We wanted to determine whether human handling of adult rabbits could induce a degree of habituation, reducing stress and facilitating research-related manipulation. To this end, adult New Zealand white rabbits were handled either frequently or minimally. After being handled over 3 wk, these rabbits were evaluated by novel personnel and compared with minimally handled controls. Evaluators subjectively scored the rabbits for their relative compliance or resistance to being scruffed and removed from their cages, being transported to a treatment room, and their behavior at all stages of the exercise. Upon evaluation, handled rabbits scored significantly more compliant than nontreated controls. During evaluation, behaviors that the rabbits displayed when they were approached in their cages and while being handled outside their cages were recorded and compared between study groups. Handled rabbits displayed behavior consistent with a reduction in human-directed fear. This study illustrates the potential for handling to improve compliance in laboratory procedures and reduce fear-related behavior in laboratory rabbits. Such handling could be used to improve rabbit welfare through the reduction of stress and exposure to novel stimuli.

  20. Seroprevalence of Encephalitozoon cuniculi in Humans and Rabbits in China.

    Directory of Open Access Journals (Sweden)

    Yaoqian Pan

    2015-06-01

    Full Text Available Encephalitozoon cuniculi is a microsporidian parasite commonly found in rabbits that can infect humans, causing encephalitozoonosis. Our objective in this study was to evaluate the seroprevalence of this parasite in rabbits and humans in China.Overall, 300 serum samples each from clinically healthy rabbit and human were collected from three regions of China (Sichuan Province, Chongqing Municipality and Jilin Province from January to September 2013 and tested for anti-E. Cuniculi antibodies using an ELISA.An overall seroprevalence of E. cuniculi was recorded as 56/300 (18.76% and 29/300 (9.76% in rabbit and human sera, respectively. The seropositivity of rabbit samples collected from Jilin province was 41%, which was significantly higher (P<0.01 than Sichuan Province (9% and Chongqing Municipality (6%. Three breeds of rabbit were used in the present study and antibody detection in Rex Rabbit was significantly (P<0.01 higher than Japanese White and New Zealand Rabbit. In human, Jilin province was more prevalent (18% followed by Sichuan Province (6% and Chongqing Municipality (5%.The E. cuniculi was present and widespread among healthy rabbits and humans in China.

  1. Abstracts of the 27th Hungarian conference on rabbit production

    Directory of Open Access Journals (Sweden)

    Abstracts, Conferences, Congresses, Symposiums...

    2015-12-01

    Full Text Available About 100 participants took part in the 27th Hungarian Conference on Rabbit Production at Kaposvár, organised by the Kaposvár University, the Hungarian Branch of the WRSA and the Rabbit Production Board. This is the largest and most popular event for rabbit breeders in Hungary. Thirteen papers were presented by senior and young scientists. Topics of the papers covered almost all fields of rabbit research (production, housing and welfare, reproduction, genetics, nutrition. Full papers are available from the organiser (matics.zsolt@ke.hu on request.

  2. Fattening and slaughter traits of four rabbit genotypes

    OpenAIRE

    Silvester Zgur; Ajda Kermauner

    2010-01-01

    Growth rate of 4 genotypes, two lines of Slovene SIKA meat rabbit (maternal line A and sire line C, both 50 rabbits), hybrid AxC (50 rabbits) and crossbred AxCal (A and Californian, 38 rabbits) was recorded from weaning (35 days of age) to slaughter (93 days). Growth performance and slaughter traits of AxC were different from line A (live weight at 91st day: A 2720, C 3158, AxC 3043 g (P£0.05), heterosis effect (H) +3.38%; carcass weight: A 1490, C 1787, AxC 1716 g (P£0.05), H +5....

  3. Rapid depletion of marbofloxacin residues in rabbit after therapeutic treatment.

    Science.gov (United States)

    Ligabue, Matteo; Lucchetti, Dario; Catone, Tiziana; Fabrizi, Laura; Marvasi, Luigi; Zaghini, Anna; Coni, Ettore

    2005-11-01

    Although rabbit meat production represents a very small percentage of the world meat market, this percentage has been growing continuously during the last 30 years. Rabbit is considered a minor food species, and therefore no drugs are specifically registered for this animal. This situation encourages rabbit farmers to make off-label use of antibacterial drugs authorized for food-producing animal species other than rabbits. In the present study, the distribution and elimination of the fluoroquinolone antibacterial agent marbofloxacin in rabbit muscle, liver, and kidney was investigated. Marbofloxacin was chosen as a representative of a new generation of antibacterial drugs active against most gram-positive and gram-negative bacteria and mycoplasms; it is well tolerated and has short elimination times in bovine and swine species. Rabbits were treated with marbofloxacin at 2 mg kg of body weight(-1) for 5 days. Residual concentrations in liver, kidney, and muscle tissues were determined posttreatment with high-performance liquid chromatography and fluorescence detection. Marbofloxacin was rapidly distributed and eliminated from rabbit tissues. Concentrations were higher in the liver and kidney than in muscle. However, 48 h after the end of treatment, marbofloxacin concentrations dropped below the maximum residue level fixed for this antibacterial drug in cattle and pigs. Considering the efficacy of marbofloxacin for the treatment of the most common rabbit diseases, its tolerability, and its short elimination time as verified in the present study, use of this antibacterial drug could be extended to therapeutic treatment of rabbits.

  4. Abstracts of the 24th Hungary conference on rabbit production

    Directory of Open Access Journals (Sweden)

    24TH Conference on rabbit production Kaposvár, Hungary. 30th May, 2012

    2014-09-01

    Full Text Available Some 100 guests took part in the 24th Hungarian Conference on Rabbit Production in Kaposvár, organised by the University of Kaposvár, the Hungarian Branch of the WRSA and the Rabbit Production Board. This is the largest and most popular event for rabbit breeders in Hungary. Seventeen papers were presented, both by senior and young scientists. Topics of the papers covered all fields of rabbit production (production, housing and welfare, reproduction, genetics, nutrition, meat quality and pathology. Full papers are available from the organiser (matics.zsolt@ke.hu on request.

  5. Rabbit haemorrhagic disease: are Australian rabbits (Oryctolagus cuniculus) evolving resistance to infection with Czech CAPM 351 RHDV?

    Science.gov (United States)

    Elsworth, P G; Kovaliski, J; Cooke, B D

    2012-11-01

    Rabbit haemorrhagic disease is a major tool for the management of introduced, wild rabbits in Australia. However, new evidence suggests that rabbits may be developing resistance to the disease. Rabbits sourced from wild populations in central and southeastern Australia, and domestic rabbits for comparison, were experimentally challenged with a low 60 ID50 oral dose of commercially available Czech CAPM 351 virus - the original strain released in Australia. Levels of resistance to infection were generally higher than for unselected domestic rabbits and also differed (0-73% infection rates) between wild populations. Resistance was lower in populations from cooler, wetter regions and also low in arid regions with the highest resistance seen within zones of moderate rainfall. These findings suggest the external influences of non-pathogenic calicivirus in cooler, wetter areas and poor recruitment in arid populations may influence the development rate of resistance in Australia.

  6. Performance of broiler fed pure glycerine

    Directory of Open Access Journals (Sweden)

    Dássia Daiane Oliveira

    2014-02-01

    Full Text Available Two experiments evaluated the pure glycerin in broiler chicken diets. Experiment 1 was a metabolism test using total feces sampling method with 96 male chickens aging from 17 to 25 d when animals were fed on two treatments: diet1 = no glycerin and diet2 = 60g/kg of glycerin. The apparent metabolized energy measured 4015 kcal/kg and the apparent metabolized corrected for nitrogen balance was 3911 kcal/kg. Experiment 2 evaluated weight gains, feed intake and feed conversion in 480 chicks at 6, 20 and 34 d old fed on diets with 0, 40, 80 and 120 g/kg of glycerin. The results indicate that pure glycerin in chicken diets, as a source of energy must take into consideration the age of the animals and it may be added up to 120 g/kg, from 20 to 41 d of age.

  7. Protection against myxomatosis and rabbit viral hemorrhagic disease with recombinant myxoma viruses expressing rabbit hemorrhagic disease virus capsid protein.

    OpenAIRE

    Bertagnoli, Stéphane; Gelfi, Jacqueline; Le Gall, Ghislaine; Boilletot, Eric; Vautherot, Jean-François; Rasschaert, Denis; Laurent, Sylvie; Petit, Frédérique; Boucraut-Baralon, Corine; Milon, Alain

    1996-01-01

    Two myxoma virus-rabbit hemorrhagic disease virus (RHDV) recombinant viruses were constructed with the SG33 strain of myxoma virus to protect rabbits against myxomatosis and rabbit viral hemorrhagic disease. These recombinant viruses expressed the RHDV capsid protein (VP60). The recombinant protein, which is 60 kDa in size, was antigenic, as revealed by its reaction in immunoprecipitation with antibodies raised against RHDV. Both recombinant viruses induced high levels of RHDV- and myxoma vir...

  8. A Study of End-Fed Arrays

    CERN Document Server

    Kondylis, Konstantinos; Zimourtopoulos, Petros

    2010-01-01

    This paper presents a general analysis of end-fed space arrays with application to self-standing linear arrays of parallel dipoles. A number of test array models were simulated, constructed and their radiation pattern was then measured. The experimental and computational results were found to be in good agreement. The developed software applications are available through the Internet as FLOSS Free Libre Open Source Software.

  9. Harmonic analysis of Doubly Fed Induction Generators

    DEFF Research Database (Denmark)

    Lindholm, Morten; Rasmussen, Tonny Wederberg

    2003-01-01

    This paper gives an overview of the frequency spectrum of the stator and rotor currents in a doubly fed induction generator (DFIG) used in wind power applications. The paper also presents a method to eliminate higher harmonics and interharmonics in the DFIG stator current. The method is implemented...... on a 40 kW laboratory model connected to the utility-grid, where the DFIG is supplied by a back-to-back 3-level NPC-converter....

  10. The Effects of Agave fourcroydes Powder as a Dietary Supplement on Growth Performance, Gut Morphology, Concentration of IgG, and Hematology Parameters in Broiler Rabbits

    Science.gov (United States)

    Iser, Maidelys; Martínez, Yordan; Jiang, Hongmei; Valdivié Navarro, Manuel; Wu, Xiaosong; Al-Dhabi, Naif Abdullah; Rosales, Manuel; Duraipandiyan, Veeramuthu

    2016-01-01

    This study was conducted to determine the effects of Agave fourcroydes powder as a dietary supplement on the growth performance, gut morphology, serum concentration of IgG, and the hematology parameters of broiler rabbits. A total of 32 rabbits [New Zealand × Californian] were weaned at 35 days. They were randomly selected for two dietary treatments (eight repetitions per treatment), which consisted of a basal diet and a basal diet supplemented with 1.5% dried-stem powder of A. fourcroydes. On day 60 from the initiation of treatment, gut histomorphology (duodenum and cecum), serum concentration of IgG, and hematology parameters were all measured. The results showed that A. fourcroydes powder supplementation improved (P < 0.05) the ADFI, ADG, and final BW. Correspondingly, this treatment increased (P < 0.05) the muscle and mucosa thickness and height and width of villi. However, duodenum crypts depth was lower (P < 0.05) when rabbits were fed with this natural product, compared with the basal diet treatment. Results also indicated that the A. fourcroydes powder increased (P < 0.05) the serum concentration of IgG but did not change the hematology parameters. This data indicates that A. fourcroydes powder, as a supplement, had beneficial effects on increasing the growth performance and serum concentration of IgG, as well as improving the gut morphology without affecting the hematology parameters in broiler rabbits. PMID:27777945

  11. Effect of dietary organic zinc sources on growth performance, incidence of diarrhoea, serum and tissue zinc concentrations, and intestinal morphology in growing rabbits

    Directory of Open Access Journals (Sweden)

    J.Y. Yan

    2017-03-01

    Full Text Available This study was conducted to evaluate the effect of dietary organic zinc (Zn sources on growth performance, the incidence of diarrhoea, serum and tissue Zn concentration, and intestinal morphology in growing rabbits. A total of 120 New Zealand White rabbits aged 35 d and with an initial body weight of 755±15 g, were randomly divided into 4 treatment groups for a 49 d feeding trial. Dietary treatments were designed with different Zn supplements as follows: (1 Control group: 80 mg/kg Zn as ZnSO4; (2 ZnLA group: 80 mg/kg Zn as Zn lactate; (3 ZnMet group: 80 mg/kg Zn as Zn methionine; (4 ZnGly group: 80 mg/kg Zn as Zn glycine. The results showed that, when compared with rabbits fed ZnSO4, supplementation with ZnLA improved (P4. Supplementing with ZnLA increased duodenum villi height (681.63 vs. 587.14 μm, P4, except that feeding ZnMet led to higher (P4. The results indicated that supplementation with 80 mg/kg Zn as ZnLA could improve growth performance, increase liver Zn concentration and enhance duodenum morphology, while reducing the incidence of diarrhoea in growing rabbits.

  12. The Effects of Agave fourcroydes Powder as a Dietary Supplement on Growth Performance, Gut Morphology, Concentration of IgG, and Hematology Parameters in Broiler Rabbits.

    Science.gov (United States)

    Iser, Maidelys; Martínez, Yordan; Ni, Hengjia; Jiang, Hongmei; Valdivié Navarro, Manuel; Wu, Xiaosong; Al-Dhabi, Naif Abdullah; Rosales, Manuel; Duraipandiyan, Veeramuthu; Fang, Jun

    2016-01-01

    This study was conducted to determine the effects of Agave fourcroydes powder as a dietary supplement on the growth performance, gut morphology, serum concentration of IgG, and the hematology parameters of broiler rabbits. A total of 32 rabbits [New Zealand × Californian] were weaned at 35 days. They were randomly selected for two dietary treatments (eight repetitions per treatment), which consisted of a basal diet and a basal diet supplemented with 1.5% dried-stem powder of A. fourcroydes. On day 60 from the initiation of treatment, gut histomorphology (duodenum and cecum), serum concentration of IgG, and hematology parameters were all measured. The results showed that A. fourcroydes powder supplementation improved (P < 0.05) the ADFI, ADG, and final BW. Correspondingly, this treatment increased (P < 0.05) the muscle and mucosa thickness and height and width of villi. However, duodenum crypts depth was lower (P < 0.05) when rabbits were fed with this natural product, compared with the basal diet treatment. Results also indicated that the A. fourcroydes powder increased (P < 0.05) the serum concentration of IgG but did not change the hematology parameters. This data indicates that A. fourcroydes powder, as a supplement, had beneficial effects on increasing the growth performance and serum concentration of IgG, as well as improving the gut morphology without affecting the hematology parameters in broiler rabbits.

  13. The Effects of Agave fourcroydes Powder as a Dietary Supplement on Growth Performance, Gut Morphology, Concentration of IgG, and Hematology Parameters in Broiler Rabbits

    Directory of Open Access Journals (Sweden)

    Maidelys Iser

    2016-01-01

    Full Text Available This study was conducted to determine the effects of Agave fourcroydes powder as a dietary supplement on the growth performance, gut morphology, serum concentration of IgG, and the hematology parameters of broiler rabbits. A total of 32 rabbits [New Zealand × Californian] were weaned at 35 days. They were randomly selected for two dietary treatments (eight repetitions per treatment, which consisted of a basal diet and a basal diet supplemented with 1.5% dried-stem powder of A. fourcroydes. On day 60 from the initiation of treatment, gut histomorphology (duodenum and cecum, serum concentration of IgG, and hematology parameters were all measured. The results showed that A. fourcroydes powder supplementation improved (P<0.05 the ADFI, ADG, and final BW. Correspondingly, this treatment increased (P<0.05 the muscle and mucosa thickness and height and width of villi. However, duodenum crypts depth was lower (P<0.05 when rabbits were fed with this natural product, compared with the basal diet treatment. Results also indicated that the A. fourcroydes powder increased (P<0.05 the serum concentration of IgG but did not change the hematology parameters. This data indicates that A. fourcroydes powder, as a supplement, had beneficial effects on increasing the growth performance and serum concentration of IgG, as well as improving the gut morphology without affecting the hematology parameters in broiler rabbits.

  14. Apoptosis of peripheral blood leukocytes from rabbits infected with non-haemagglutinating strains of rabbit haemorrhagic disease virus (RHDV).

    Science.gov (United States)

    Niedźwiedzka-Rystwej, Paulina; Deptuła, Wiesław

    2012-09-15

    The report demonstrates that the induction of apoptosis in peripheral blood granulocytes and lymphocytes of rabbits infected with three non-haemagglutinating RHDV strains (English Rainham, German Frankfurt, and Spanish Asturias) is a crucial determinant of the pathogenesis of rabbit haemorrhagic disease. Apoptosis was measured by flow cytometric detection of caspase activity. These studies demonstrated that the investigated RHDV (rabbit haemorrhagic disease virus) viral strains affected leukocyte apoptosis to varying degrees. Enhanced leukocyte apoptosis was detected between 4 and 36 h after infection and was more pronounced in lymphocytes than in granulocytes. The data presented here thus provide a preliminary understanding of the kinetics of apoptosis in leukocytes of rabbits infected with RHDV.

  15. Detection of L1, infectious virions and anti-L1 antibody in domestic rabbits infected with cottontail rabbit papillomavirus.

    Science.gov (United States)

    Hu, Jiafen; Budgeon, Lynn R; Cladel, Nancy M; Culp, Timothy D; Balogh, Karla K; Christensen, Neil D

    2007-12-01

    Shope papillomavirus or cottontail rabbit papillomavirus (CRPV) is one of the first small DNA tumour viruses to be characterized. Although the natural host for CRPV is the cottontail rabbit (Sylvilagus floridanus), CRPV can infect domestic laboratory rabbits (Oryctolagus cuniculus) and induce tumour outgrowth and cancer development. In previous studies, investigators attempted to passage CRPV in domestic rabbits, but achieved very limited success, leading to the suggestion that CRPV infection in domestic rabbits was abortive. The persistence of specific anti-L1 antibody in sera from rabbits infected with either virus or viral DNA led us to revisit the questions as to whether L1 and infectious CRPV can be produced in domestic rabbit tissues. We detected various levels of L1 protein in most papillomas from CRPV-infected rabbits using recently developed monoclonal antibodies. Sensitive in vitro infectivity assays additionally confirmed that extracts from these papillomas were infectious. These studies demonstrated that the CRPV/New Zealand White rabbit model could be used as an in vivo model to study natural virus infection and viral life cycle of CRPV and not be limited to studies on abortive infections.

  16. Coccidian and nematode infections influence prevalence of antibody to myxoma and rabbit hemorrhagic disease viruses in European rabbits.

    Science.gov (United States)

    Bertó-Moran, Alejandro; Pacios, Isabel; Serrano, Emmanuel; Moreno, Sacramento; Rouco, Carlos

    2013-01-01

    The interaction among several parasites in European rabbits (Oryctolagus cuniculus) is crucial to host fitness and to the epidemiology of myxomatosis and rabbit hemorrhagic disease. These diseases have caused significant reductions in rabbit populations on the Iberian Peninsula. Most studies have focused on the epidemiology and pathogenesis of these viruses individually, and little is known about interactions between these viruses and other parasites. Taking advantage of an experimental restocking program in Spain, the effects of coccidian and nematode infections on the probability of having detectable antibody to myxoma and rabbit hemorrhagic disease viruses were tested in European wild rabbits. For 14 mo, we monitored rabbit abundance and parasite loads (coccidia and nematodes) in three reintroduced rabbit populations. While coccidian and nematode loads explained seasonal antibody prevalences to myxoma virus, the pattern was less clear for rabbit hemorrhagic disease. Contrary to expectations, prevalence of antibody to myxoma virus was inversely proportional to coccidian load, while nematode load seemed to play a minor role. These results have implications for viral disease epidemiology and for disease management intended to increase rabbit populations in areas where they are important for ecosystem conservation.

  17. Protection against myxomatosis and rabbit viral hemorrhagic disease with recombinant myxoma viruses expressing rabbit hemorrhagic disease virus capsid protein.

    Science.gov (United States)

    Bertagnoli, S; Gelfi, J; Le Gall, G; Boilletot, E; Vautherot, J F; Rasschaert, D; Laurent, S; Petit, F; Boucraut-Baralon, C; Milon, A

    1996-08-01

    Two myxoma virus-rabbit hemorrhagic disease virus (RHDV) recombinant viruses were constructed with the SG33 strain of myxoma virus to protect rabbits against myxomatosis and rabbit viral hemorrhagic disease. These recombinant viruses expressed the RHDV capsid protein (VP60). The recombinant protein, which is 60 kDa in size, was antigenic, as revealed by its reaction in immunoprecipitation with antibodies raised against RHDV. Both recombinant viruses induced high levels of RHDV- and myxoma virus-specific antibodies in rabbits after immunization. Inoculations by the intradermal route protected animals against virulent RHDV and myxoma virus challenges.

  18. Environmental sensitivity differs between rabbit lines selected for reproductive intensity and longevity.

    Science.gov (United States)

    Savietto, D; Cervera, C; Blas, E; Baselga, M; Larsen, T; Friggens, N C; Pascual, J J

    2013-12-01

    To better understand the mechanisms that allow some animals to sustain their productive effort in harsh environmental conditions, rabbit does from two selection lines (LP and V) were housed in normal (NC), nutritional (NF) or heat (HC) challenging environmental conditions from first to third partum. The LP line (n=85) was founded on reproductive longevity criteria by selecting does from commercial farms that had a minimum of 25 partum with more than 7.5 kits born alive per parity. Line V (n=79) was constituted from four specialised maternal lines into a composite synthetic line and then selected by litter size at weaning for 36 generations. Female rabbits in NC and NF environments were housed at normal room temperature (18°C to 24°C) and fed with control [11.6 MJ digestible energy (DE)/kg dry matter (DM)] or low-energy diets (9.1 MJ DE/kg DM). HC does were housed at high room temperatures (25°C to 35°C) and fed the control diet. Female rabbits in the HC and NF environments ingested 11.5% and 6% less DE than NC does, respectively (P<0.05). These differences between environments occurred in both lines, with the differences being higher for LP than for V does (+6%; P<0.05). Milk yield responses followed those of energy intake also being higher for LP does (+21.3 g/day; P<0.05). The environmental conditions did not affect the perirenal fat thickness (PFT), but a genotype by environment interaction was observed. In NC and HC, the PFT was higher for line V (+0.23 and +0.35 mm, respectively; P<0.05) than for LP does, but this was not the case at NF (-0.01 mm). Moreover, the PFT evolution was different between them. In the NC environment, LP does used the accreted PFT in late lactation (-0.29 mm), whereas V does did not (-0.08 mm). Conversely, in the HC environment, LP does showed a flat PFT evolution in late lactation, whereas V does accumulated PFT. In the NF environment, LP and V does had a similar PFT evolution. There was also a litter size reduction for V does of

  19. Market Driving to Develop Rabbit Meat Products in Indonesia

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    Atien Priyanti

    2012-09-01

    Full Text Available Rabbit meat is a nutritional food containing high protein and low cholesterol, fat and sodium. Current research in rabbit production is aimed for developing production strategies to increase the nutritional and economic values of rabbit meat products as functional food. Nowadays, producing rabbit is a popular farming activity in many parts of Indonesia as a small and medium scale operation for food security and cash income. Rabbit farming is to produce meat, skin and hides, fur, organic fertilizers and pet or fancy animals. Consumption of rabbit meat is considered very low, due partly to low meat supply and inavailability of marketing. In some tourist areas, such as Lembang (West Java, Tawangmangu (Central Java, Sarangan and Batu (East Java rabbit meat is a specific food. Attempt to create and drive rabbit markets will simultaneously increase meat production to fulfill the demand and meet economic scale of farming. Hence, this will give significant impact to the farmers’ welfare. Availability of good quality meat, dissemination and diversification of meat products, production efficiency toward competitive price along with its proper marketing strategy will drive consumers’ preferences to consume more rabbit meat. Market driving needs to be created in order to promote rabbit meat products by establishing food outlets. This program has been developed by a farmers group in Magelang, Central Java. During the period of 2006 – 2007 the food outlets had increased to 5 outlets, and in 2012 become 9 outlets. This market driving will also have an impact on changing orientation of rabbit farming from traditional to a small and medium economic scale that will influence the production efficiency.

  20. Poikilocytosis in rabbits: prevalence, type, and association with disease.

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    Mary M Christopher

    Full Text Available Rabbits (Oryctolagus cuniculus are a popular companion animal, food animal, and animal model of human disease. Abnormal red cell shapes (poikilocytes have been observed in rabbits, but their significance is unknown. The objective of this study was to investigate the prevalence and type of poikilocytosis in pet rabbits and its association with physiologic factors, clinical disease, and laboratory abnormalities. We retrospectively analyzed blood smears from 482 rabbits presented to the University of California-Davis Veterinary Medical Teaching Hospital from 1990 to 2010. Number and type of poikilocytes per 2000 red blood cells (RBCs were counted and expressed as a percentage. Acanthocytes (>3% of RBCs were found in 150/482 (31% rabbits and echinocytes (>3% of RBCs were found in 127/482 (27% of rabbits, both healthy and diseased. Thirty-three of 482 (7% rabbits had >30% acanthocytes and echinocytes combined. Mild to moderate (>0.5% of RBCs fragmented red cells (schistocytes, microcytes, keratocytes, spherocytes were found in 25/403 (6% diseased and 0/79 (0% healthy rabbits (P = 0.0240. Fragmentation and acanthocytosis were more severe in rabbits with inflammatory disease and malignant neoplasia compared with healthy rabbits (P<0.01. The % fragmented cells correlated with % polychromasia, RDW, and heterophil, monocyte, globulins, and fibrinogen concentrations (P<0.05. Echinocytosis was significantly associated with renal failure, azotemia, and acid-base/electrolyte abnormalities (P<0.05. Serum cholesterol concentration correlated significantly with % acanthocytes (P<0.0001, % echinocytes (P = 0.0069, and % fragmented cells (P = 0.0109, but correlations were weak (Spearman ρ <0.02. These findings provide important insights into underlying pathophysiologic mechanisms that appear to affect the prevalence and type of naturally-occurring poikilocytosis in rabbits. Our findings support the need to carefully document poikilocytes in research

  1. Myxomatosis in farmland rabbit populations in England and Wales.

    Science.gov (United States)

    Ross, J; Tittensor, A M; Fox, A P; Sanders, M F

    1989-10-01

    The overall pattern and consequences of myxomatosis in wild rabbit populations were studied at three farmland sites in lowland southern England and upland central Wales between 1971 and 1978. When results from all years were combined, the disease showed a clear two-peaked annual cycle, with a main autumn peak between August and January, and a subsidiary spring peak during February to April. Rabbit fleas, the main vectors of myxomatosis in Britain, were present on full-grown rabbits in sufficient numbers for transmission to occur throughout the year, but the observed seasonal pattern of the disease appeared to be influenced by seasonal mass movements of these fleas. However other factors were also important including the timing and success of the main rabbit breeding season, the proportion of rabbits which had recovered from the disease and the timing and extent of autumn rabbit mortality from other causes. Significantly more males than females, and more adults and immatures than juveniles, were observed to be infected by myxomatosis. Only 25-27% of the total populations were seen to be infected during outbreaks. Using two independent methods of calculation, it was estimated that between 47 and 69% of infected rabbits died from the disease (much lower than the expected 90-95% for fully susceptible rabbits with the partly attenuated virus strains that predominated). Thus it was estimated that 12-19% of the total rabbit populations were known to have died directly or indirectly from myxomatosis. Although the effects of myxomatosis were much less than during the 1950s and 1960s, it continued to be an important mortality factor. It may still have a regulatory effect on rabbit numbers, with autumn/winter peaks of disease reducing the numbers of rabbits present at the start of the breeding season.

  2. Effects of plant extracts and essential oils as feed supplements on quality and microbial traits of rabbit meat

    Directory of Open Access Journals (Sweden)

    A. P. Kone

    2016-06-01

    Full Text Available The effects of dietary supplementation of onion, cranberry,  strawberry and essentials oils on meat quality were analysed. Five groups of 48 Grimaud female weaned rabbits received the supplemented or the control ration; the experimental unit was a cage of 6 rabbits. Each experimental diet contained 10 ppm of added active ingredients.  Rabbits were fed with the experimental diets for 4 wk before determining slaughter and carcass traits and determining the pH at 1 and 24 h post mortem (pHu of the Longissimus dorsi (LD and the Biceps femoris (BF muscle, left and right, respectively. Cooking loss, drip loss and L*, a* and b* color parameters were obtained of the right LD and for ground meat and antioxidant status (TBARS, DNPH, Folin Ciocalteu. Only the pHu of the LD muscle for the strawberry supplemented group was significantly lower when compared to the control group (P=0.04. However, we note that for the pH of the LD, the average was less than 6 for the meat of animals who received a diet enriched in polyphenols, compared to the control group. Plant extract supplementation did not influence meat quality traits, growth performance or oxidative stability. But under aerobic and anaerobic conditions, our results indicate that diet supplementation with extracts rich in polyphenols, especially with essential oils, had a small bot sporadic positive effect in reducing bacterial microflora compared to the control group (P<0.05. In conclusion, plant extracts and essential oils can be used in a rabbit diet without adverse effects on performance and meat quality traits. This effect could be optimized by investigating higher doses.

  3. Optimisation of reference genes for gene-expression analysis in a rabbit model of left ventricular diastolic dysfunction.

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    Walid Nachar

    Full Text Available Left ventricular diastolic dysfunction (LVDD is characterized by the disturbance of ventricle's performance due to its abnormal relaxation or to its increased stiffness during the diastolic phase. The molecular mechanisms underlying LVDD remain unknown. We aimed to identify normalization genes for accurate gene-expression analysis of LVDD using quantitative real-time PCR (RT-PCR in a new rabbit model of LVDD. Eighteen rabbits were fed with a normal diet (n = 7 or a 0.5% cholesterol-enriched diet supplemented with vitamin D2 (n = 11 for an average of 14.5 weeks. We validated the presence of LVDD in this model using echocardiography for diastolic function assessment. RT-PCR was performed using cDNA derived from left ventricle samples to measure the stability of 10 genes as candidate reference genes (Gapdh, Hprt1, Ppia, Sdha, Rpl5, Actb, Eef1e1, Ywhaz, Pgk1, and G6pd. Using geNorm analysis, we report that Sdha, Gapdh and Hprt1 genes had the highest stability (M <0.2. By contrast, Hprt1 and Rpl5 genes were found to represent the best combination for normalization when using the Normfinder algorithm (stability value of 0.042. Comparison of both normalization strategies highlighted an increase of natriuretic peptides (Bnp and Anp, monocytes chemotactic protein-1 (Mcp-1 and NADPH oxidase subunit (Nox-2 mRNA expressions in ventricle samples of the hypercholesterolemic rabbits compared to controls (P<0.05. This increase correlates with LVDD echocardiographic parameters and most importantly it molecularly validates the presence of the disease in our model. This is the first study emphasizing the selection of stable reference genes for RT-PCR normalization in a rabbit model of LVDD.

  4. Estimating Apparent Nutrient Digestibility of Diets Containing Leucaena leucocephala or Moringa oleifera Leaf Meals for Growing Rabbits by Two Methods.

    Science.gov (United States)

    Safwat, A M; Sarmiento-Franco, L; Santos-Ricalde, R H; Nieves, D; Sandoval-Castro, C A

    2015-08-01

    This study aimed to evaluate the nutrient digestibility of growing rabbits fed diets with different levels of either Leucaena leucocephala (LLM) or Moringa oleifera (MOLM) leaf meals and also to compare total collection and TiO2 marker methods for estimating digestibility. A total of 30 California growing rabbits (1.81±0.19 kg live weight on average) were randomly distributed into five experimental groups of six rabbits each and were housed in individual cages. The groups were control, 30% LLM, 40% LLM, 30% MOLM, and 40% MOLM. All groups received pelleted diets for two weeks; diets also contained 4 g/kg titanium dioxide as dietary marker. Daily feed intake was recorded during the whole experimental period and total feces were collected daily and weighed individually during four days. The results showed that there were no difference (p>0.05) in feed, dry matter (DM), organic matter (OM), crude protein (CP), digestible energy, and crude fiber (CF) intake between the control group and the other experimental groups. The apparent digestibility values of DM, OM, CP, CF, acid detergent fiber, and gross energy were the highest for control group (p = 0.001), meanwhile MOLM diets had generally higher nutrient digestibility coefficients than LLM diets. Increasing the inclusion level of leaf meal in the diet from 30% to 40% improved the digestibility of CF from 45.02% to 51.69% for LLM and from 48.11% to 55.89% for MOLM. Similar results for apparent nutrient digestibility coefficients were obtained when either total collection or indigestible marker method was used. In conclusion, the digestibility of MOLM containing diets were better than LLM diets, furthermore TiO2 as an external marker could be used as a simple, practical and reliable method to estimate nutrients digestibility in rabbit diets.

  5. A robust rabbit line increases leucocyte counts at weaning and reduces mortality by digestive disorder during fattening.

    Science.gov (United States)

    García-Quirós, A; Arnau-Bonachera, A; Penadés, M; Cervera, C; Martínez-Paredes, E; Ródenas, L; Selva, L; Viana, D; Corpa, J M; Pascual, J J

    2014-10-15

    The present work evaluates how a rabbit line selected for robustness and two other lines selected for productive criteria, could have affected the physiological maturity and blood leukocytes counts of young rabbits at weaning, as well as their possible effect on the subsequent performance and health status during the growing period. The study was conducted on a total of 2904 young rabbits weaned at 30 days, belonging to three different genetic types (line H, founded for litter size at birth and selected for litter size at weaning during 17 generations; line LP, characterised by robustness founded for reproductive longevity criteria and selected for litter size at weaning for 7 generations; and line R, founded and selected during 25 generations for average daily gain from the 4th to the 9th week of life). Two different diets were used during lactation. The two diets were both isoenergetic and isoprotein but their main energy source differed, being either animal fat (AF) or cereal starch (CS). Leucocyte subsets were characterised at weaning, and growing performance was studied until 58 days of age (feed intake, live weight, mortality by digestive disorders and morbidity) for both medicated and non-medicated dietary versions. At weaning, young rabbits fed an AF lactating diet evidenced greater B lymphocyte count (on av. +8.6 ± 3.5 × 10(6)/L; P rabbits, blood from LP ones had higher counts for total (on av. 591 ± 167 × 10(6)/L; P rabbits also showed lower mortality by digestive disorders (on av. -8 points of percentage) and morbidity (on av. -4 points) than those from H and R lines during the growing period (P < 0.05). R animals presented higher feed intake and daily weight gain, and a lower feed conversion ratio than H and LP animals (on av. +16.7 ± 2.7 g dry matter/day, +10.3 ± 0.4 g/day and -0.22 ± 0.04 g dry matter/g, respectively). In conclusion, the foundation of a line for reproductive longevity, which has been previously reported to give greater

  6. Apoptosis of peripheral blood leucocytes in rabbits infected with different strains of rabbit haemorrhagic disease virus.

    Science.gov (United States)

    Niedźwiedzka-Rystwej, Paulina; Hukowska-Szematowicz, Beata; Tokarz-Deptuła, Beata; Trzeciak-Ryczek, Alicja; Działo, Joanna; Deptuła, Wiesław

    2013-01-01

    The pathogenicity of RHDV (rabbit haemorrhagic disease virus) is mainly associated with its affinity to blood vessels, with causing disseminated intravascular coagulations (DIC), and with the stimulation of the host immune system. Moreover, there are implications suggesting that apoptosis may be a pivotal process in understanding the basis of viral haemorrhagic disease in rabbits - a serious infectious disease causing mortality to wild and domestic rabbits. The aim of this study is to evaluate, by means of flow cytometry, the dynamics of apoptosis in peripheral blood granulocytes and lymphocytes in rabbits experimentally infected with seven different strains of RHDV and so-called antigenic variants of RHDV denominated as RHDVa, i.e.: Hungarian 24V/89, 1447V/96, 72V/2003; Austrian 01-04, 237/04, V-412 and French 05-01. The results showed that all of the RHDV and RHDVa strains cause an increase in the number of apoptotic cells throughout the infection, which might indicate the need for further analysis of the importance of this process.

  7. Surface Ig on rabbit lymphocytes. Rabbit B and T cells are distinct populations

    NARCIS (Netherlands)

    Bast, B J; Catty, D; Manten-Slingerland, R; Jansen, J T; Veldhuis, Dick H.; Roholl, P; Ballieux, R E

    1979-01-01

    Rabbit peripheral blood lymphocytes (PBL) were analyzed by immunofluorescence using anti-T cell conjugates and anti-Fab, anti-a1 allotype, anti-IgM and anti-IgA conjugates. In addition, T cells were demonstrated by rosetting with papain-treated homologous erythrocytes. Control experiments, using aci

  8. Pea-derived vaccines demonstrate high immunogenicity and protection in rabbits against rabbit haemorrhagic disease virus.

    Science.gov (United States)

    Mikschofsky, Heike; Schirrmeier, Horst; Keil, Günther M; Lange, Bodo; Polowick, Patricia L; Keller, Wilf; Broer, Inge

    2009-08-01

    Vaccines against rabbit haemorrhagic disease virus (RHDV) are commercially produced in experimentally infected rabbits. A genetically engineered and manufactured version of the major structural protein of RHDV (VP60) is considered to be an alternative approach for vaccine production. Plants have the potential to become an excellent recombinant production system, but the low expression level and insufficient immunogenic potency of plant-derived VP60 still hamper its practical use. In this study, we analysed the expression of a novel multimeric VP60-based antigen in four different plant species, including Nicotiana tabacum L., Solanum tuberosum L., Brassica napus L. and Pisum sativum L. Significant differences were detected in the expression patterns of the novel fusion antigen cholera toxin B subunit (CTB)::VP60 (ctbvp60(SEKDEL)) at the mRNA and protein levels. Pentameric CTB::VP60 molecules were only detected in N. tabacum and P. sativum, and displayed equal levels of CTB, at approximately 0.01% of total soluble protein (TSP), and traces of detectable VP60. However, strong enhancement of the CTB protein content via self-fertilization was only observed in P. sativum, where it reached up to 0.7% of TSP. In rabbits, a strong decrease in the protective vaccine dose required from 48-400 microg potato-derived VP60 [Castanon, S., Marin, M.S., Martin-Alonso, J.M., Boga, J.A., Casais, R., Humara, J.M., Ordas, R.J. and Parra, F. (1999) Immunization with potato plants expressing VP60 protein protects against rabbit hemorrhagic disease virus. J. Virol. 73, 4452-4455; Castanon, S., Martin-Alonso, J.M., Marin, M.S., Boga, J.A., Alonso, P., Parra, F. and Ordas, R.J. (2002) The effect of the promoter on expression of VP60 gene from rabbit hemorrhagic disease virus in potato plants. Plant Sci. 162, 87-95] to 0.56-0.28 microg antigenic VP60 (measured with VP60 enzyme-linked immunosorbent assay) of crude CTB::VP60 pea extracts was demonstrated. Rabbits immunized with pea-derived CTB

  9. Protecting short-term intravascular ear catheters in healthy rabbits.

    Science.gov (United States)

    Sampieri, Francesca; Orchard, Rekha N; Antonopoulos, Aphroditi J; Hamilton, Donald L

    2012-01-20

    Researchers may place a catheter in the ear vessel of a rabbit for a short period of time in order to collect repeated blood samples without extensive restraint of the animal. Maintaining such a catheter in a healthy rabbit can be challenging, as the animal may scratch at the ear, removing the catheter or forming a large hematoma that might impede blood sampling. The authors developed a technique for protecting the indwelling catheter by cutting a section of moleskin to the same shape as the ear and gluing it to the surface of the ear and the catheter. They applied this technique to collect multiple blood samples during 12-h periods from nine rabbits in a pharmacokinetics study. Catheters remained patent in five rabbits for 12 h, in two rabbits for 8 h, in one rabbit for 6 h and in one rabbit for 4 h. This technique allowed for collection of repeated blood samples and prevented the rabbits from interfering with the catheter while allowing them to move freely during the sampling period.

  10. Infection in rabbits with the Lyme disease spirochete.

    Science.gov (United States)

    Kornblatt, A. N.; Steere, A. C.; Brownstein, D. G.

    1984-01-01

    Of 33 rabbits inoculated with Lyme disease spirochetes, two developed erythema chronicum migrans at the site of inoculation. Spirochetes were seen in skin biopsies of one of the lesions with immunoperoxidase and Warthin-Starry stains. Spirochetes were also recovered from the blood of two additional rabbits two weeks post-inoculation. These findings are characteristic of early Lyme disease in humans. PMID:6393613

  11. Clinical symptoms and diagnosis of encephalitozoonosis in pet rabbits.

    Science.gov (United States)

    Künzel, Frank; Gruber, Andrea; Tichy, Alexander; Edelhofer, Renate; Nell, Barbara; Hassan, Jasmin; Leschnik, Michael; Thalhammer, Johann G; Joachim, Anja

    2008-02-14

    Infections with Encephalitozoon cuniculi in rabbits are observed at increasing frequency and are known as opportunistic infections in immunocompromised humans. 191 pet rabbits with suspected encephalitozoonosis, presented at the Animal Hospital of the Veterinary University of Vienna (Austria), were included in this study. Rabbits were serologically examined for antibodies against E. cuniculi (144 positive out of 184 rabbits with suspected encephalitozoonosis compared to 14 positive out of 40 clinically healthy rabbits tested as part of a standard health check) and Toxoplasma gondii (8 positive out of 157). Of the 144 seropositive rabbits with clinical signs, 75% showed neurological symptoms, 14.6% demonstrated phacoclastic uveitis and 3.5% suffered from renal failure. 6.9% of the animals had combined symptoms. Vestibular disease dominated within the rabbits that showed neurological symptoms. Polymerase chain reaction (PCR) could not detect parasite DNA in urine or cerebrospinal fluid (CSF), but did so in 4 out of 5 samples of liquefied lens material in cases with phacoclastic uveitis due to lens capsule rupture. Additionally further diagnostic procedures, such as inspection of the external ear canal (N=69), radiography of the tympanic bullae (N=65) were performed to rule out differential diagnosis. 54.2% of the patients exhibiting neurological symptoms recovered within a few days, while 87.5% of the rabbits suffering from renal failure died or had to be euthanized.

  12. Digestive efficiency in rabbit does according to environment and genetic type

    Directory of Open Access Journals (Sweden)

    Davi Savietto

    2012-10-01

    Full Text Available Ninety lactating rabbit does of three different genetic types [2 from a line differentiating 20 generations by selection for litter size at weaning (lines V16 and V36 and 1 from a line founded under reproductive longevity criteria and then selected by litter size at weaning (line LP] were subjected to three environmental conditions: NC, females housed under normal conditions (14 to 20oC and fed with a control diet (333 g NDF/kg DM; HC, females housed under heat conditions (25 to 35oC and fed with the control diet; or NF, females housed under normal conditions and fed with a fibrous diet (443 g NDF/kg DM.  The apparent digestible coefficients of dry matter (DMd, organic matter, crude protein (CPd, gross energy, NDF (NDFd and acid detergent fibre, as well as the daily intake of dry matter (DM, digestible protein (DP and digestible energy (DE, were determined (14 to 18 d post-partum.  The environment affected all variables analysed.  In general, heat conditions reduced the daily feed intake (around -30%; P<0.05 and increased main apparent digestible coefficients (+4.5 percentage points for DMd.  In contrast, the use of a fibrous diet led to lower DE intake (-217 kJ/d; P<0.05 and main apparent digestibility coefficients (-13.5 percentage points for DMd.  Females from line V, regardless of generation, showed lower daily DM intake (-19.2 g/d; P<0.05 and NDFd (-1.5 percentage points; P<0.05 than line LP.  Interactions between genetic type and environment were found for daily DM intake, NDFd and CPd.  When receiving fibrous diet, LP females showed a higher increment in daily DM intake (65.6 g/d; P<0.05 than V36 females, compared to control. Under heat conditions, NDFd obtained for LP females were higher to those in normal conditions (+3.14 percentage points, while V females showed similar NDFd.  In addition, the increase in CPd observed under heat conditions was higher for LP (+9.87 percentage points and V36 (+8.74 percentage points than V16

  13. Experimental model of bladder instability in rabbits

    Directory of Open Access Journals (Sweden)

    Balasteghin K.T.

    2003-01-01

    Full Text Available OBJECTIVE: Propose a new experimental model of bladder instability in rabbits after partial bladder obstruction. MATERIALS AND METHODS: Thirty North Folk male rabbits, weighting 1,700 to 2,820 g (mean: 2,162 g were studied. The animals were distributed in 2 experimental groups, formed by 15 rabbits each: Group 1 - clinical control. In this group there was no surgical intervention; Group 2 - bladder outlet obstruction. In this group, after anesthetizing the animal, urethral cannulation with Foley catheter 10F was performed and then an adjustable plastic bracelet was passed around the bladder neck. It was then adjusted in order to not constrict the urethra. The following parameters were studied in M1 - pre-operative period; M2 - 4 weeks post-operatively moments: 1- urine culture; 2- cystometric study; 3- serum creatinine and BUN. RESULTS: Bladder weight was 2.5 times larger in the group with obstruction than in the control group. Cystometric evaluation showed a significant increase in maximal vesical volume in the final moment at Group G2. However, there was no statistically significant difference among the groups studied. There was no statistically significant difference between maximal detrusor pressure and vesical compliance in the different moments or in the studied groups. There was an absence of uninhibited detrusor contractions in all the animals in group 1, and involuntary contractions were detected in 93% of group 2 animals. There was no significant variation in BUN and serum creatinine either among the groups or in the same group. CONCLUSIONS: We observed in the group with obstruction a bladder weight 2.5 higher than normal bladders. We detected involuntary contractions in 93% of the animals in group 2, establishing this experimental model as appropriate to secondary bladder instability and partial bladder outlet obstruction.

  14. Rabbit haemorrhagic disease (RHD and rabbit haemorrhagic disease virus (RHDV: a review

    Directory of Open Access Journals (Sweden)

    Abrantes Joana

    2012-02-01

    Full Text Available Abstract Rabbit haemorrhagic disease virus (RHDV is a calicivirus of the genus Lagovirus that causes rabbit haemorrhagic disease (RHD in adult European rabbits (Oryctolagus cuniculus. First described in China in 1984, the virus rapidly spread worldwide and is nowadays considered as endemic in several countries. In Australia and New Zealand where rabbits are pests, RHDV was purposely introduced for rabbit biocontrol. Factors that may have precipitated RHD emergence remain unclear, but non-pathogenic strains seem to pre-date the appearance of the pathogenic strains suggesting a key role for the comprehension of the virus origins. All pathogenic strains are classified within one single serotype, but two subtypes are recognised, RHDV and RHDVa. RHD causes high mortality in both domestic and wild adult animals, with individuals succumbing between 48-72 h post-infection. No other species has been reported to be fatally susceptible to RHD. The disease is characterised by acute necrotising hepatitis, but haemorrhages may also be found in other organs, in particular the lungs, heart, and kidneys due to disseminated intravascular coagulation. Resistance to the disease might be explained in part by genetically determined absence or weak expression of attachment factors, but humoral immunity is also important. Disease control in rabbitries relies mainly on vaccination and biosecurity measures. Such measures are difficult to be implemented in wild populations. More recent research has indicated that RHDV might be used as a molecular tool for therapeutic applications. Although the study of RHDV and RHD has been hampered by the lack of an appropriate cell culture system for the virus, several aspects of the replication, epizootology, epidemiology and evolution have been disclosed. This review provides a broad coverage and description of the current knowledge on the disease and the virus.

  15. Rabbit haemorrhagic disease (RHD) and rabbit haemorrhagic disease virus (RHDV): a review

    Science.gov (United States)

    2012-01-01

    Rabbit haemorrhagic disease virus (RHDV) is a calicivirus of the genus Lagovirus that causes rabbit haemorrhagic disease (RHD) in adult European rabbits (Oryctolagus cuniculus). First described in China in 1984, the virus rapidly spread worldwide and is nowadays considered as endemic in several countries. In Australia and New Zealand where rabbits are pests, RHDV was purposely introduced for rabbit biocontrol. Factors that may have precipitated RHD emergence remain unclear, but non-pathogenic strains seem to pre-date the appearance of the pathogenic strains suggesting a key role for the comprehension of the virus origins. All pathogenic strains are classified within one single serotype, but two subtypes are recognised, RHDV and RHDVa. RHD causes high mortality in both domestic and wild adult animals, with individuals succumbing between 48-72 h post-infection. No other species has been reported to be fatally susceptible to RHD. The disease is characterised by acute necrotising hepatitis, but haemorrhages may also be found in other organs, in particular the lungs, heart, and kidneys due to disseminated intravascular coagulation. Resistance to the disease might be explained in part by genetically determined absence or weak expression of attachment factors, but humoral immunity is also important. Disease control in rabbitries relies mainly on vaccination and biosecurity measures. Such measures are difficult to be implemented in wild populations. More recent research has indicated that RHDV might be used as a molecular tool for therapeutic applications. Although the study of RHDV and RHD has been hampered by the lack of an appropriate cell culture system for the virus, several aspects of the replication, epizootology, epidemiology and evolution have been disclosed. This review provides a broad coverage and description of the current knowledge on the disease and the virus. PMID:22325049

  16. Significant reduction of the antiatherogenic effect of estrogen by long-term inhibition of nitric oxide synthesis in cholesterol-clamped rabbits.

    Science.gov (United States)

    Holm, P; Korsgaard, N; Shalmi, M; Andersen, H L; Hougaard, P; Skouby, S O; Stender, S

    1997-01-01

    The purpose of this study was to investigate whether endothelium-derived nitric oxide (NO) is involved in the plasma lipid-independent antiatherogenic effect of estrogen and levormeloxifene, a partial estrogen receptor agonist. 85 rabbits were ovariectomized and balloon-injured in the middle thoracic aorta. The rabbits were fed a cholesterol-enriched diet supplemented with 17beta-estradiol, levormeloxifene, or placebo, either alone, or together with 160 microg/ml NG-nitro- -arginine methyl ester (-NAME), an NO synthase inhibitor, in their drinking water for 12 wk. Plasma cholesterol was maintained at 25-30 mmol/liter by individualized cholesterol feeding. In the undamaged aorta, the extent of atherosclerosis in the estrogen group was only one-third that in the placebo group. Simultaneous administration of -NAME, however, significantly reduced the antiatherogenic effect of estrogen (P < 0.01). There was no significant difference between the placebo group given -NAME and the group treated with placebo alone. At the previously endothelium-denuded site, estrogen had no effect on atherosclerosis development, whereas -NAME combined with estrogen significantly increased atherogenesis (P < 0.05). The effects of levormeloxifene were almost similar to those of estrogen. Active vascular concentrations of -NAME were demonstrated in an additional study, in which maximal aortic/coronary endothelium-dependent relaxation was significantly inhibited in rabbits given -NAME. Thus, in this study a considerable part of the plasma lipid-independent antiatherogenic effect of estrogen was mediated through its effect on endothelial NO in cholesterol-fed rabbits. The results for levormeloxifene suggest a common mechanism of action for estrogen and partial estrogen receptor agonists on atherogenesis. PMID:9259581

  17. Strange Curves, Counting Rabbits, & Other Mathematical Explorations

    CERN Document Server

    Ball, Keith

    2011-01-01

    How does mathematics enable us to send pictures from space back to Earth? Where does the bell-shaped curve come from? Why do you need only 23 people in a room for a 50/50 chance of two of them sharing the same birthday? In Strange Curves, Counting Rabbits, and Other Mathematical Explorations, Keith Ball highlights how ideas, mostly from pure math, can answer these questions and many more. Drawing on areas of mathematics from probability theory, number theory, and geometry, he explores a wide range of concepts, some more light-hearted, others central to the development of the field and used dai

  18. International Conference on Immunogenetics of the Rabbit.

    Science.gov (United States)

    1983-12-09

    2.~ .* - ,.. 2"? - - .=--. • L A- 12 Genetics and expression of kappa and lambda light chains in BASILEA rabbits. N. McCartney-Francis, G. 0...the bas mRNA also reveal two distinct light chain RNA specie): kappa with the expected size of 1.15 kb and lambda of 1.04 kb size. Poly(A) RNAs of... kappa light chain * with the b5 (97-108) region determined for the first time in this work suggest that this portion, similar to the 96-1l7 3 region

  19. Surgical Management of Ear Diseases in Rabbits.

    Science.gov (United States)

    Csomos, Rebecca; Bosscher, Georgia; Mans, Christoph; Hardie, Robert

    2016-01-01

    Otitis externa and media are frequently diagnosed disorders in rabbits and are particularly common in lop-eared breeds because of the specific anatomy of the ear canal. Medical management for otitis externa and media often provides only a temporary improvement in clinical signs. Surgery by means of partial or total ear canal ablation (PECA or TECA) combined with lateral bulla osteotomy (LBO) represents a feasible approach that is well tolerated and provides a good clinical outcome. Short-term complications associated with PECA/TECA-LBO include facial nerve paralysis and vestibular disease.

  20. GROWTH ANALYSIS IN RABBIT USING GOMPERTZ NON-LINEAR MODEL

    Directory of Open Access Journals (Sweden)

    A. Setiaji

    2014-10-01

    Full Text Available An experiment was conducted to compare the growth curve of rabbit. Three breeds of rabbit,namely Indonesian Local Rabbit (IL, Flamish Giant (FG and Rex (R were used in the study.Individual body weights of each breed was measured from birth to 63 days of age with 3-days interval.Those periodical data were separated into different sex, be then it was averaged to analysis growthpattern. Growth curve parameters were estimated to fit growth data. There was no difference in bodyweight between sexs within breed. Indonesian local rabbit had the lowest body weight. The resultsshowed that growth curve paramaters among three breeds were significantly different (P<0.05 for bothsexes. FG had the highest value of asymptotic mature weight, followed by R and IL. In conclusion,Gompertz model was excellent fit for the growth data in rabbit with a high coefficient determination (R2= 0.999.

  1. Kinetic analysis of apolipoproteins in postprandial hypertriglyceridaemia rabbits.

    Science.gov (United States)

    Hata, M; Ito, T; Ohwada, K

    2009-04-01

    The postprandial hypertriglyceridaemia (PHT) rabbit, developed as a new animal model of metabolic syndrome, is characterized by PHT, central obesity and glucose intolerance. For detailed investigation of lipid metabolism characteristics in PHT rabbit, the plasma levels of apolipoproteins A-I, B, C-II, C-III and E were measured. Movements of apolipoproteins B100 and B48 were investigated using sodium dodecyl sulphate-polyacrylamide gel electrophoresis to determine whether postprandially increased triglyceride is exogenous or endogenous. The level of apolipoproteins A-I, B, C-II and E were increased in PHT rabbit after feeding. Apolipoproteins B100 and B48 were detected in the plasma fraction of d triglyceride lipase were deficient and that the hepatic uptake of exogenous lipoproteins was delayed in the PHT rabbit. Especially, for examining remnant hyperlipoproteinaemia in humans, PHT rabbit is an excellent animal model for hypertriglyceridaemia research.

  2. Preliminary research on myosin light chain kinase in rabbit liver

    Institute of Scientific and Technical Information of China (English)

    Bin Ren; Hua-Qing Zhu; Zhao-Feng Luo; Qing Zhou; Yuan Wang; Yu-Zhen Wang

    2001-01-01

    AIM: To study preliminarily the properties of myosin light chain kinase (MLCK) in rabbit liver. METHODS: The expression of MLCK was detected by reverse transcription-polymerase chain reaction (RT-PCR);the MLCK was obtained from rabbit liver, and its activity was analyzed by γ-32P incorporation technique to detect the phosphorylation of myosin light chain. RESULTS: MLCK was expressed in rabbit liver, and the activity of the enzyme was similar to rabbit smooth muscle MLCK, and calmodulin-dependent. When the concentration was 0.65 mg-L-1, the activity was at the highest level. CONCLUSION: MLCK expressed in rabbit liver may catalyze the phosphorylation of myosin light chain, which may play important roles in the regulation of hepatic cell functions.

  3. Faecal Excretion of Glycosphingolipids of Breast-fed and Formula-fed Infants

    OpenAIRE

    Larson, G.; Falk, P; Hynsjö, L.; Midtvedt, A. -C.; Midtvedt, T

    2011-01-01

    Faecal samples of six healthy breast-fed, or formula-fed, infants were collected at intervals up to 9 mth of age and analysed for their contents of sphingolipids. Comparisons were made with faecal samples of one older child and one adult. The mean faccal content of sphingolipids during the first 9 mth of life (8.2 μmol sphingosine/g dry stool) was significantly less than that of meconium samples (23.3 μmol/gj but higher than that of adult stools (1.2 μmol/g). Sphingomyelin excretion ...

  4. Cystoscopic diagnosis of polypoid cystitis in two pet rabbits.

    Science.gov (United States)

    Di Girolamo, Nicola; Bongiovanni, Laura; Ferro, Silvia; Melidone, Raffaele; Nicoletti, Annalisa; Duca, Valeria Del; Donnelly, Thomas M; Selleri, Paolo

    2017-07-01

    CASE DESCRIPTION AS-year-old male Dwarf rabbit and 4-year-old female Mini-Rex rabbit were evaluated because of anorexia and urine scalding of the perineum. CLINICAL FINDINGS Abdominal radiography revealed a diffuse increase in the opacity of the urinary bladder attributable to urinary sludge. In 1 rabbit, abdominal ultrasonography revealed several mass-like lesions protruding from the mucosal surface into the lumen of the urinary bladder. Rabbits were anesthetized, and cystoscopy was performed with a rigid 2.7-mm, 30° endoscope. Histologic analysis of tissue samples obtained through the cystoscope operating channel revealed findings consistent with polypoid cystitis. TREATMENT AND OUTCOME To remove the urinary sludge from each rabbit, the urinary bladder was filled with sterile saline (0.9% NaCl) solution and emptied with a gentle massage several times until the ejected fluid was transparent. Rabbits were treated with NSAIDs, antimicrobials (chosen following microbial culture of urine and antimicrobial susceptibility testing), bathing of the perineum, and a low-calcium diet. The male rabbit died of unrelated causes 18 months later; postmortem examination findings confirmed the polypoid cystitis. The female rabbit remained disease free through to last follow-up (12 months after initial evaluation). CLINICAL RELEVANCE This was the first report of polypoid cystitis in pet rabbits. Although ultrasonographic findings supported this diagnosis, a definitive diagnosis was achieved through cystoscopy and lesion biopsy. Treatments administered were intended to reduce the potential sources of irritation. Research is needed to investigate the effectiveness of the applied interventions and the association between excessive urinary calcium excretion and polyploid cystitis in rabbits.

  5. Transgenic rabbits as therapeutic protein bioreactors and human disease models.

    Science.gov (United States)

    Fan, Jianglin; Watanabe, Teruo

    2003-09-01

    Genetically modified laboratory animals provide a powerful approach for studying gene expression and regulation and allow one to directly examine structure-function and cause-and-effect relationships in pathophysiological processes. Today, transgenic mice are available as a research tool in almost every research institution. On the other hand, the development of a relatively large mammalian transgenic model, transgenic rabbits, has provided unprecedented opportunities for investigators to study the mechanisms of human diseases and has also provided an alternative way to produce therapeutic proteins to treat human diseases. Transgenic rabbits expressing human genes have been used as a model for cardiovascular disease, AIDS, and cancer research. The recombinant proteins can be produced from the milk of transgenic rabbits not only at lower cost but also on a relatively large scale. One of the most promising and attractive recombinant proteins derived from transgenic rabbit milk, human alpha-glucosidase, has been successfully used to treat the patients who are genetically deficient in this enzyme. Although the pronuclear microinjection is still the major and most popular method for the creation of transgenic rabbits, recent progress in gene targeting and animal cloning has opened new avenues that should make it possible to produce transgenic rabbits by somatic cell nuclear transfer in the future. Based on a computer-assisted search of the studies of transgenic rabbits published in the English literature here, we introduce to the reader the achievements made thus far with transgenic rabbits, with emphasis on the application of these rabbits as human disease models and live bioreactors for producing human therapeutic proteins and on the recent progress in cloned rabbits.

  6. High rabbit abundance proves detrimental to the population growth rate in European rabbit (Oryctolagus cuniculus L. extensive breeding enclosures

    Directory of Open Access Journals (Sweden)

    L. Ruiz-Aizpurua

    2014-09-01

    Full Text Available The European rabbit (Oryctolagus cuniculus L. is a key prey species in Mediterranean ecosystems that has declined in its natural ranges as a result of diseases and loss of habitat. This situation has led to the production of wild rabbits in enclosures in which they can acclimate and breed. The efficiency of these enclosures as extensive breeding systems is defined by their population growth rate (PGR. The aim of this study is to analyse the effect of rabbit abundance on the PGR. This has been done by creating general linear models to explain autumn and spring PGR with the use of rabbit abundance estimates, enclosure size, aerial predation and previous PGR as possible explanatory variables. Rabbit abundance and enclosure size negatively affected the autumn PGR, while only rabbit abundance affected the spring PGR in the best-fit models. It is suggested that maintaining rabbit densities at fewer than 30 rabbits per hectare might help to optimise the efficiency inside enclosures.

  7. Confirmation and phylogenetic analysis of rabbit hemorrhagic disease virus in free-living rabbits from the Netherlands

    NARCIS (Netherlands)

    van de Bildt, M. W. G.; van Bolhuis, G. H.; van Zijderveld, F.; van Riel, D.; Drees, J. M.; Osterhaus, A. D. M. E.; Kuiken, T.

    2006-01-01

    The number of free-living European rabbits (Oryctolagus cuniculus) in the Netherlands has declined dramatically in recent years. Although rabbit hemorrhagic disease virus (RHDV) infection has been implicated as a possible cause of this decline, the definitive diagnosis has not been reported. We exam

  8. Confirmation and phylogenetic analysis of rabbit hemorrhagic disease virus in free-living rabbits from the Netherlands

    NARCIS (Netherlands)

    van de Bildt, M. W. G.; van Bolhuis, G. H.; van Zijderveld, F.; van Riel, D.; Drees, J. M.; Osterhaus, A. D. M. E.; Kuiken, T.

    2006-01-01

    The number of free-living European rabbits (Oryctolagus cuniculus) in the Netherlands has declined dramatically in recent years. Although rabbit hemorrhagic disease virus (RHDV) infection has been implicated as a possible cause of this decline, the definitive diagnosis has not been reported. We

  9. Exocrine pancreatic secretion is stimulated in piglets fed Fish oil compared with those fed Coconut Oil or Lard

    DEFF Research Database (Denmark)

    Hedemann, Mette Skou; Pedersen, Asger Roer; Engberg, Ricarda M.

    2001-01-01

    An experiment was conducted to study the effect of feeding diets containing fat sources with different fatty acid composition (fish oil, coconut oil or lard, 10 g/100 g diet) on exocrine pancreatic secretion in piglets after weaning. A total of 16 barrows were weaned at 4 wk of age; 3 d later...... the coconut oil or lard diets. The output [U/(h. kg(0.75))] of lipase was higher in piglets fed fish oil than in piglets fed lard or coconut oil. The output of colipase was greater in piglets fed fish oil and coconut oil than in those fed lard. The dietary treatments did not affect the output of carboxylester...... hydrolase. The output of trypsin was significantly lower in piglets fed lard than in piglets fed fish oil or coconut oil diets and the output of carboxypeptidase B was greater in those fed the fish oil diet. Protein, chymotrypsin, carboxypeptidase A, elastase and amylase outputs did not differ among...

  10. Increased virulence of rabbit haemorrhagic disease virus associated with genetic resistance in wild Australian rabbits (Oryctolagus cuniculus).

    Science.gov (United States)

    Elsworth, Peter; Cooke, Brian D; Kovaliski, John; Sinclair, Ronald; Holmes, Edward C; Strive, Tanja

    2014-09-01

    The release of myxoma virus (MYXV) and Rabbit Haemorrhagic Disease Virus (RHDV) in Australia with the aim of controlling overabundant rabbits has provided a unique opportunity to study the initial spread and establishment of emerging pathogens, as well as their co-evolution with their mammalian hosts. In contrast to MYXV, which attenuated shortly after its introduction, rapid attenuation of RHDV has not been observed. By studying the change in virulence of recent field isolates at a single field site we show, for the first time, that RHDV virulence has increased through time, likely because of selection to overcome developing genetic resistance in Australian wild rabbits. High virulence also appears to be favoured as rabbit carcasses, rather than diseased animals, are the likely source of mechanical insect transmission. These findings not only help elucidate the co-evolutionary interaction between rabbits and RHDV, but reveal some of the key factors shaping virulence evolution. Copyright © 2014 Elsevier Inc. All rights reserved.

  11. Body morphometric development during growth and maturity of coloured dwarf rabbits available in the Italian market

    Directory of Open Access Journals (Sweden)

    Antonella Dalle Zotte

    2013-12-01

    Full Text Available This study was designed to measure the live performance of “coloured dwarf” rabbits available in the Italian market during both the growing and adult maintenance periods to provide new insights on this breed, with specific regard to the gender effect. A further objective was to identify the morphometric characteristics of the selected population and compare them to the standard breeding requirements. Body weight (BW and body morphometric development were monitored in 145 “coloured dwarf” rabbits (80 males and 65 females during 2 different periods: 7-21 wk of age (growth and 28-45 wk of age (adultmaintenance. The animals were housed in individual cages and fed ad libitum with a commercial pelleted diet. Individual BW and feed consumption were measured twice a week to determine weight gain, feed intake and feed conversion index, whereas the health status was monitored daily. At 20 and 45 wk of age, the skin fold width and body measurements were taken on each rabbit at predefined anatomical points. The body measurements included: body length (BL, ear length (EL, head height (HH, shoulder width (SW, rump width (RW, forehead width (FHW, head width (HW, tibia width (TW, thoracic circumference (TC, abdominal circumference (AC and 3 body indices: RW/BL, TC/BL, AC/BL. At 20 wk of age, the body weight of females (F was heavier than that of males (M (F: 1.630 vs. M: 1.542 g, P<0.001 and this significant difference was also maintained during the maintenance phase of the study (F: 1.953 vs. M: 1.850 g; P<0.001 at 45 wk. Interestingly, up to 20 wk of age average feed intake was higher and weight gain more rapid in F than M, whereas from 40 wk of age onwards the situation was reversed, as M registered a higher average feed intake (P<0.05. At 45 wk of age, the skin fold width was thicker in M than in F (M: 4.28 vs. F: 3.56 mm; P<0.001. All body measurements were affected by age and almost all of them also by gender, with the exception of BL, EL, FHW and

  12. A rabbit model of lower eyelid fibrosis.

    Science.gov (United States)

    Griepentrog, Gregory J; Park, D J John; Zaldivar, Renzo A; Pulido, Jose S; Cameron, J Douglas; Woog, John J

    2010-01-01

    To create and validate a new model of lower eyelid fibrosis in Dutch-belted rabbits. Five Dutch-belted rabbits were injected with a transcutaneous 1-ml injection of standard 95% ethanol alcohol just inferior to the eyelid margin of one lower eyelid. A control injection of 1 ml of balanced saline solution was given to the opposite eyelid. A small tattoo was placed on the skin overlying the inferior orbital rim and used as a measuring point of reference in relation to the lower eyelid margin. Analysis was twofold: eyelid measurements were made over 8 weeks to determine the presence of eyelid shortening, and a histopathologic analysis was performed. Mean lower eyelid shortening was greater in the ethanol alcohol intervention eyelids than the control group (-3.4 mm +/- 1.67 mm vs. 0.5 mm +/- 0.71 mm, p = 0.01). Histopathologic analysis revealed extensive fibrosis in the ethanol alcohol invention eyelids compared with the control group. Ethanol alcohol induces eyelid fibrosis and lower eyelid shortening. This may be a useful model in the future testing of novel surgical or pharmacologic treatments.

  13. Residual stress distribution in rabbit limb bones.

    Science.gov (United States)

    Yamada, Satoshi; Tadano, Shigeru; Fujisaki, Kazuhiro

    2011-04-29

    The presence of the residual stresses in bone tissue has been noted and the authors have reported that there are residual stresses in bone tissue. The aim of our study is to measure the residual stress distribution in the cortical bone of the extremities of vertebrates and to describe the relationships with the osteon population density. The study used the rabbit limb bones (femur, tibia/fibula, humerus, and radius/ulna) and measured the residual stresses in the bone axial direction at anterior and posterior positions on the cortical surface. The osteons at the sections at the measurement positions were observed by microscopy. As a result, the average stresses at the hindlimb bones and the forelimb bones were 210 and 149 MPa, respectively. In the femur, humerus, and radius/ulna, the residual stresses at the anterior position were larger than those at the posterior position, while in the tibia, the stress at the posterior position was larger than that at the anterior position. Further, in the femur and humerus, the osteon population densities in the anterior positions were larger than those in the posterior positions. In the tibia, the osteon population density in the posterior position was larger than that in the anterior position. Therefore, tensile residual stresses were observed at every measurement position in the rabbit limb bones and the value of residual stress correlated with the osteon population density (r=0.55, P<0.01).

  14. Amiodarone Induced Morphological Changes in Rabbit Pneumocytes

    Directory of Open Access Journals (Sweden)

    Fereshteh Mehraein

    2009-01-01

    Full Text Available Objective: Amiodarone as an iodinated benzofuran derivative is a potent antiarrhythmicagent currently used for the treatment of ventricular arrhythmias. Pulmonary toxicityis one of the complications of Amiodarone therapy. The aim of this study was todetermine the toxicity of Amiodarone for pneumocytes.Materials and Methods: 14 male white New Zealand rabbits were divided in a controlgroup and an experimental group. The experimental group was subjected to intraperitoneal injection with a single daily dose of 80 mg/kg Amiodarone for two weeks.The control group received only normal saline. At the end of the injection period, thetwo groups were anesthetized and perfused with Karnovsky fixative. The lung tissuewas removed and fixed, then prepared for light and electron microscope studies.Morphometric studies were made on sections to find nucleus profile dimensions.Results: Light microscope observation showed acute changes in the alveolus includingcongestion of alveolar capillaries and infiltration of red blood cells (RBCs intothe lumen of the alveoli. Electron microscope study of lung tissue revealed abnormalinclusion bodies within type ΙΙ & Ι pneumocytes. The micrographs also showedthe presence of vacuoles in 5% of the type ΙΙ pneumocytes. Morphometric studiesshowed that the nucleus of the cells in the experimental group were smaller than inthe control group (p<0.01.Conclusion: These results indicate that Amiodarone administration can cause damageto pnuemocytes and the alveolus of rabbit lung, so the effectiveness of Amiodaronein long term treatment of heart failure patients is limited because of the developmentof lung toxicity.

  15. Complexation of arsenic species in rabbit erythrocytes.

    Science.gov (United States)

    Delnomdedieu, M; Basti, M M; Styblo, M; Otvos, J D; Thomas, D J

    1994-01-01

    The binding of arsenite, As(III), and arsenate, As(V), by molecules in the intracellular compartment of rabbit erythrocytes has been studied by 1H- and 31P-NMR spectroscopy, uptake of 73As, and ultrafiltration experiments. For intact erythrocytes to which 0.1-0.4 mM arsenite was added, direct evidence was obtained for entry of 76% within 1/2 h and subsequent binding of As(III) by intracellular glutathione and induced changes in the hemoglobin structure (NMR), likely due to binding of As(III). These results were compared with the effect of addition of As(V) on intact erythrocytes and revealed that a smaller amount of As(V) (approximately 25%) enters the cells; the main fraction of As(V) enters the phosphate pathway, depletes ATP, and increases Pi. In contrast, As(III) did not affect the ATP level. Both 1H- and 31P-NMR data indicated striking differences between As(III) and As(V) behavior when incubated with rabbit erythrocytes. These differences were confirmed by 73As uptake and binding experiments. meso-2,3-Dimercaptosuccinic acid (DMSA), a dithiol ligand, released glutathione from its arsenite complexes in erythrocytes.

  16. Effect of freezing on rabbit cultured chondrocytes

    Directory of Open Access Journals (Sweden)

    R.R Filgueiras

    2011-02-01

    Full Text Available This work evaluated the effect of freezing on chondrocytes maintained in culture, aiming the establishment of a cell bank for future application as heterologous implant. Chondrocytes extracted from joint cartilage of nine healthy New Zealand White rabbits were cultivated and frozen with the cryoprotector 5% dimethylsulfoxide for six months. Phenotypic and scanning electron microscopy analyses were carried out to identify morphological and functional differences between fresh and thawed cells. After enzymatic digestion, a total of 4.8x10(5cells per rabbit were obtained. Fresh chondrocytes showed a high mitotic rate and abundant matrix was present up to 60 days of culture. Loss of phenotypic stability was notable in the thawed chondrocytes, with a low labeling of proteoglycans and weak immunostaining of type II collagen. The present study showed important loss of chondrocyte viability under the freezing conditions. For future in vivo studies of heterologous implant, these results suggests that a high number of cells should be implanted in the host site in order to achieve an adequate number of viable cells. Furthermore, the chondrocytes should be implanted after two weeks of culture, when the highest viability rate is found

  17. Analysis of gene expression in rabbit muscle

    Directory of Open Access Journals (Sweden)

    Alena Gálová

    2014-02-01

    Full Text Available Increasing consumer knowledge of the link between diet and health has raised the demand for high quality food. Meat and meat products may be considered as irreplaceable in human nutrition. Breeding livestock to higher content of lean meat and the use of modern hybrids entails problems with the quality of meat. Analysing of livestock genomes could get us a great deal of important information, which may significantly affect the improvement process. Domestic animals are invaluable resources for study of the molecular architecture of complex traits. Although the mapping of quantitative trait loci (QTL responsible for economically important traits in domestic animals has achieved remarkable results in recent decades, not all of the genetic variation in the complex traits has been captured because of the low density of markers used in QTL mapping studies. The genome wide association study (GWAS, which utilizes high-density single-nucleotide polymorphism (SNP, provides a new way to tackle this issue. New technologies now allow producing microarrays containing thousands of hybridization probes on a single membrane or other solid support. We used microarray analysis to study gene expression in rabbit muscle during different developmental age stages. The outputs from GeneSpring GX sotware are presented in this work. After the evaluation of gene expression in rabbits, will be selected genes of interest in relation to meat quality parameters and will be further analyzed by the available methods of molecular biology and genetics.

  18. Heart rate variability in isolated rabbit hearts.

    Science.gov (United States)

    Frey, B; Heger, G; Mayer, C; Kiegler, B; Stöhr, H; Steurer, G

    1996-11-01

    The presence of heart rate variability (HRV) in patients with cardiac denervation after heart transplantation raised our interest in HRV of isolated, denervated hearts. Hearts from seven adult white ELCO rabbits were transferred to a perfusion apparatus. All hearts were perfused in the working mode and in the Langendorff mode for 20 minutes each. HRV was analyzed in the frequency domain. A computer simulated test ECG at a constant rate of 2 Hz was used for error estimation of the system. In the isolated, denervated heart, HRV was of random, broadband fluctuations, different from the well-characterized oscillations at specific frequencies in intact animals. Mean NN was 423 +/- 51 ms in the Langendorff mode, 406 +/- 33 ms in the working heart mode, and 500 ms in the test ECG. Total power was 663 +/- 207 ms2, 817 +/- 318 ms2, and 3.7 ms2, respectively. There was no significant difference in any measure of HRV between Langendorff and working heart modes. The data provide evidence for the presence of HRV in isolated, denervated rabbit hearts. Left atrial and ventricular filling, i.e., the working heart mode, did not alter HRV, indicating that left atrial or ventricular stretch did not influence the sinus nodal discharge rate.

  19. Metallothionein in rabbit kidneys preserved for transplantation

    Energy Technology Data Exchange (ETDEWEB)

    Elinder, C.G.; Lundgren, G.; Nordberg, M.; Palm, B.; Piscator, M.

    1984-03-01

    Thirteen rabbits were given cadmium injections to achieve cadmium concentrations in kidney cortex ranging from 0.05 to 1 mmole Cd/kg wet weight. Another four animals served as controls. One kidney from each animal was frozen directly to -70/sup 0/C whereas the other kidney was kept for 24 hr at +4/sup 0/C in a preservative (Sachs' solution) to simulate conditions for preservation of human donor kidneys before transplantation. Protein binding of cadmium, zinc and copper in kidney homogenates and the concentration of metallothionein (MT) were measured in the kidney that was frozen directly and in the kidney that had been preserved. No gross differences in either the protein binding of cadmium, zinc and copper or in the MT content were seen between the directly frozen and preserved kidney from the same animal. This indicates that MT is not rapidly broken down in rabbit kidneys which have been preserved similarly to human donor kidneys for 24 hr in a standard preservative solution prior to a transplantation. 27 references, 3 figures, 1 table.

  20. Metallothionein in rabbit kidneys preserved for transplantation.

    Science.gov (United States)

    Elinder, C G; Lundgren, G; Nordberg, M; Palm, B; Piscator, M

    1984-03-01

    Thirteen rabbits were given repeated cadmium injections to achieve cadmium concentrations in kidney cortex ranging from 0.05 to 1 mmole Cd/kg wet weight. Another four animals served as controls. One kidney from each animal was frozen directly to -70 degrees C whereas the other kidney was kept for 24 hr at +4 degrees C in a preservative (Sachs' solution) to simulate conditions for preservation of human donor kidneys before transplantation. Protein binding of cadmium, zinc and copper in kidney homogenates and the concentration of metallothionein (MT) were measured in the kidney that was frozen directly and in the kidney that had been preserved. No gross differences in either the protein binding of cadmium, zinc and copper or in the MT content were seen between the directly frozen and preserved kidneys from the same animal. This indicates that MT is not rapidly broken down in rabbit kidneys which have been preserved similarly to human donor kidneys for 24 hr in a standard preservative solution prior to a transplantation.

  1. Converter fed sub sea motor drives

    Energy Technology Data Exchange (ETDEWEB)

    Raad, R.O.

    1995-09-01

    Minor offshore gas and oil resources located 20-50 km from existing installations may often be commercially exploited only by use of complete sub sea solutions. This thesis deals with analyses of a sub sea adjustable speed electric motor which is fed by a frequency converter via a long cable (up to 50 km) between the converter and the motor. The author develops a general model for analysing such motor drive systems with the objective of verifying the feasibility of specific applications and of specifying the requirements on the system components. The simulation model is used to identify the critical frequency ranges in which the converter must not generate significant harmonics, to verify the start-up strategy chosen, and to verify the stability with potential disturbances applied to the system. Simulation models are developed for both transient and steady state analyses. They are accurate up to 5 kHz and can incorporate the frequency dependency of the motor and cable parameters. Ideal thyristors and diodes are used. The models are implemented in existing simulation tools. Most of the results relate to a base case with a 670 kW squirrel cage motor fed from a 30 km long cable, but cases with 3 MW rating or with 50 km cable have also been analyzed and found to be feasible. Each specific application must be separately studied. Results of simulation calculations are presented and conclusions given. 53 refs., 124 figs., 23 tabs.

  2. Coupling Between Waveguide-Fed Slot Arrays

    Science.gov (United States)

    Rengarajan, Sembiam

    2011-01-01

    Coupling between two waveguide-fed planar slot arrays has been investigated using full-wave analysis. The analysis employs the method-of-moments solution to the pertinent coupled integral equations for the aperture electric field of all slots. In order to compute coupling between two arrays, the input port of the first array is excited with a TE(sub 10) mode wave while the second one is match-terminated. After solving the moment method matrix equations, the aperture fields of all slots are obtained and thereby the TE(sub 10) mode wave received at the input port of the second array is determined. Coupling between two arrays is the ratio of the wave amplitude arriving in the second array port to the incident wave amplitude at the first array port. The coupling mechanism has been studied as a function of spacing between arrays in different directions, e.g. the electric field plane, the magnetic field plane, and the diagonal plane. Computed coupling values are presented for different array geometries. This work is novel since it provides a good understanding of coupling between waveguide-fed slot arrays as a function of spacing and orientation for different aperture distributions and array architectures. This serves as a useful tool for antenna design engineers and system engineers.

  3. Innervation of the rabbit cardiac ventricles.

    Science.gov (United States)

    Pauziene, Neringa; Alaburda, Paulius; Rysevaite-Kyguoliene, Kristina; Pauza, Audrys G; Inokaitis, Hermanas; Masaityte, Aiste; Rudokaite, Gabriele; Saburkina, Inga; Plisiene, Jurgita; Pauza, Dainius H

    2016-01-01

    The rabbit is widely used in experimental cardiac physiology, but the neuroanatomy of the rabbit heart remains insufficiently examined. This study aimed to ascertain the architecture of the intrinsic nerve plexus in the walls and septum of rabbit cardiac ventricles. In 51 rabbit hearts, a combined approach involving: (i) histochemical acetylcholinesterase staining of intrinsic neural structures in total cardiac ventricles; (ii) immunofluorescent labelling of intrinsic nerves, nerve fibres (NFs) and neuronal somata (NS); and (iii) transmission electron microscopy of intrinsic ventricular nerves and NFs was used. Mediastinal nerves access the ventral and lateral surfaces of both ventricles at a restricted site between the root of the ascending aorta and the pulmonary trunk. The dorsal surface of both ventricles is supplied by several epicardial nerves extending from the left dorsal ganglionated nerve subplexus on the dorsal left atrium. Ventral accessing nerves are thicker and more numerous than dorsal nerves. Intrinsic ventricular NS are rare on the conus arteriosus and the root of the pulmonary trunk. The number of ventricular NS ranged from 11 to 220 per heart. Four chemical phenotypes of NS within ventricular ganglia were identified, i.e. ganglionic cells positive for choline acetyltransferase (ChAT), neuronal nitric oxide synthase (nNOS), and biphenotypic, i.e. positive for both ChAT/nNOS and for ChAT/tyrosine hydroxylase. Clusters of small intensely fluorescent cells are distributed within or close to ganglia on the root of the pulmonary trunk, but not on the conus arteriosus. The largest and most numerous intrinsic nerves proceed within the epicardium. Scarce nerves were found near myocardial blood vessels, but the myocardium contained only a scarce meshwork of NFs. In the endocardium, large numbers of thin nerves and NFs proceed along the bundle of His and both its branches up to the apex of the ventricles. The endocardial meshwork of fine NFs was

  4. An Empirical Analysis of Farmers’ Rabbit Breeds Purchase and Its Influencing Factors

    Institute of Scientific and Technical Information of China (English)

    Yuhe; SONG; Laping; WU

    2014-01-01

    In this paper,based on the survey data on farmers in 14 provinces and cities nationwide provided by China Rabbit Research System,we analyze the farmers’ rabbit breeds selection,purchase channels and the demand for new varieties of rabbits as well as the problems in the course of rabbit usage. We make an empirical analysis of the factors influencing farmers’ rabbit demand,and put forth the recommendations for farmers’ rabbit breeds usage and to improve the promotion of new varieties of rabbits.

  5. Seroprevalence of Encephalitozoon cuniculi in Humans and Rabbits in China.

    Science.gov (United States)

    Pan, Yaoqian; Wang, Shuai; Liu, Xingyou; Li, Ruizhen; Sun, Yuqian; Gadahi, Javaid Ali

    2015-01-01

    Encephalitozoon cuniculi is a microsporidian parasite commonly found in rabbits that can infect humans, causing encephalitozoonosis. Our objective in this study was to evaluate the seroprevalence of this parasite in rabbits and humans in China. Overall, 300 serum samples each from clinically healthy rabbit and human were collected from three regions of China (Sichuan Province, Chongqing Municipality and Jilin Province) from January to September 2013 and tested for anti-E. Cuniculi antibodies using an ELISA. An overall seroprevalence of E. cuniculi was recorded as 56/300 (18.76%) and 29/300 (9.76%) in rabbit and human sera, respectively. The seropositivity of rabbit samples collected from Jilin province was 41%, which was significantly higher (Prabbit were used in the present study and antibody detection in Rex Rabbit was significantly (PRabbit. In human, Jilin province was more prevalent (18%) followed by Sichuan Province (6%) and Chongqing Municipality (5%). The E. cuniculi was present and widespread among healthy rabbits and humans in China.

  6. Impact of Pregnancy on Zonisamide Pharmacokinetics in Rabbits

    Directory of Open Access Journals (Sweden)

    Kamal M. Matar

    2013-01-01

    Full Text Available Pregnancy is associated with various physiological changes which may lead to significant alterations in the pharmacokinetics of many drugs. The present study was aimed to investigate the potential effects of pregnancy on the pharmacokinetic profile of zonisamide (ZNM in the rabbit. Seven female rabbits were used in this study. The pregnant and nonpregnant rabbits received ZNM orally at a dose of 10 mg/kg and blood samples were collected from the animals just before receiving the drug and then serially for up to 24 h. The plasma samples were analyzed using tandem mass spectrometric method. Following a single oral dose of ZNM to the rabbits, the mean values of ZNM plasma concentrations at different times were consistently low in pregnant compared to nonpregnant rabbits. The mean values of ZNM’s Cmax and AUC0-∞ were significantly (P<0.05 decreased, whereas the CL/F exhibited substantial increase (P<0.05 in pregnant compared to nonpregnant rabbits. Tmax, t1/2abs, t1/2el, MRT, and Vd/F showed no significant differences between the two groups. The present study demonstrates that pregnancy decreased ZNM plasma concentrations in rabbits and that the decrease could be due to decreased extent of gastrointestinal absorption, induced hepatic metabolism, or enhanced renal elimination of the drug.

  7. Carob pod insoluble fiber exerts anti-atherosclerotic effects in rabbits through sirtuin-1 and peroxisome proliferator-activated receptor-γ coactivator-1α.

    Science.gov (United States)

    Valero-Muñoz, María; Martín-Fernández, Beatriz; Ballesteros, Sandra; Lahera, Vicente; de las Heras, Natalia

    2014-09-01

    The aim of this study was to evaluate the potential effects of an insoluble dietary fiber from carob pod (IFC) (1 g ⋅ kg(-1) ⋅ d(-1) in the diet) on alterations associated with atherosclerosis in rabbits with dyslipidemia. Male New Zealand rabbits (n = 30) were fed the following diets for 8 wk: 1) a control diet (SF412; Panlab) as a control group representing normal conditions; 2) a control supplemented with 0.5% cholesterol + 14% coconut oil (DL) (SF302; Panlab) for 8 wk as a dyslipidemic group; and 3) a control containing 0.5% cholesterol + 14% coconut oil plus IFC (1 g ⋅ kg(-1) ⋅ d(-1)) (DL+IFC) for 8 wk. IFC was administered in a pellet mixed with the DL diet. The DL-fed group developed mixed dyslipidemia and atherosclerotic lesions, which were associated with endothelial dysfunction, inflammation, and fibrosis. Furthermore, sirtuin-1 (SIRT1) and peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) protein expression in the aorta were reduced to 77% and 63% of the control group, respectively (P < 0.05), in these rabbits. Administration of IFC to DL-fed rabbits reduced the size of the aortic lesion significantly (DL, 15.2% and DL+IFC, 2.6%) and normalized acetylcholine-induced relaxation (maximal response: control, 89.3%; DL, 61.6%; DL+IFC, 87.1%; P < 0.05) and endothelial nitric oxide synthase expression (DL, 52% and DL+IFC, 104% of the control group). IFC administration to DL-fed rabbits also reduced cluster of differentiation 36 (DL, 148% and DL+IFC, 104% of the control group; P < 0.05), plasminogen activator inhibitor-1 (DL, 141% and DL+IFC, 107% of the control group), tumor necrosis factor-α (DL, 166% and DL+IFC, 120% of the control group), vascular cell adhesion molecule-1 (DL, 153% and DL+IFC, 110% of the control group), transforming growth factor-β (DL, 173% and DL+IFC, 99% of the control group), and collagen I (DL, 157% and DL+IFC, 112% of the control group) in the aorta. These effects were accompanied by an enhancement of

  8. Exocrine pancreatic secretion is stimulated in piglets fed fish oil compared with those fed coconut oil or lard.

    Science.gov (United States)

    Hedemann, M S; Pedersen, A R; Engberg, R M

    2001-12-01

    An experiment was conducted to study the effect of feeding diets containing fat sources with different fatty acid composition (fish oil, coconut oil or lard, 10 g/100 g diet) on exocrine pancreatic secretion in piglets after weaning. A total of 16 barrows were weaned at 4 wk of age; 3 d later, they were surgically fitted with a catheter in the pancreatic duct for continuous collection of pancreatic juice. Collections of pancreatic juice were made every other day starting 4 d postsurgically. Piglets fed the fish oil diet secreted a significantly greater volume of pancreatic juice than piglets fed the coconut oil or lard diets. The output [U/(h. kg(0.75))] of lipase was higher in piglets fed fish oil than in piglets fed lard or coconut oil. The output of colipase was greater in piglets fed fish oil and coconut oil than in those fed lard. The dietary treatments did not affect the output of carboxylester hydrolase. The output of trypsin was significantly lower in piglets fed lard than in piglets fed fish oil or coconut oil diets and the output of carboxypeptidase B was greater in those fed the fish oil diet. Protein, chymotrypsin, carboxypeptidase A, elastase and amylase outputs did not differ among the dietary treatment groups. The apparent digestibilities of nutrients and energy were measured in feces and did not differ among groups. Thus, the greater output of lipase in fish oil-fed piglets did not result in a greater digestibility of fat in this diet.

  9. SMALL-SCALE RABBIT MEAT PRODUCTION IN THE WESTERN HEMISPHERE : BACK TO BASICS ?

    OpenAIRE

    LUKEFAHR, S.D.

    1999-01-01

    [EN] In the Western Hemisphere, in countries where the demand for rabbit meat is low, and where intensive livestock production systems are common (e.g., commercial broiler, swine, and cattle production), it may not be appropriate for rabbit scientists to recommend commercial rabbit production. Commercial-scale rabbit operations might only be justified, at best, when located in proximity to processing plants and(or) to large cities where there is a strong tradition of rabbit ...

  10. The Alice - "Follow the White Rabbit" - parasites of farm rabbits based on coproscopy.

    Science.gov (United States)

    Kornaś, Sławomir; Kowal, Jerzy; Wierzbowska, Izabela; Basiaga, Marta; Nosal, Paweł; Niedbała, Piotr

    2015-01-01

    The aim of the study, conducted in the years 2011-2013, was to determine the level of gastrointestinal parasites infection in New Zealand White rabbits, kept at the Experimental Station of the University of Agriculture in Krakow. The study showed rabbits protozoan infection with the genus Eimeria, belonging - based on the sporulation method - to the following species: E. magna, E. media, E. perforans, E. stiedae and E. irresidua. The highest prevalence of infection, as well as the intensity of oocysts output (OPG - oocysts per gram of faeces), was noted for E. magna and E. media - respectively 31.4 % (19477.3 OPG), and 40.0 % (14256.07 OPG). The infection of rabbits with Eimeria spp. differed significantly between years. With regard to oocysts output, the level of infection was strongly connected with the age of rabbits, being higher in young animals. However, the range of infection was highest among adults. Among nematodes, Passalurus ambiguus pinworm was regularly found (prevalence reached 21.9%), other species - Trichuris leporis, and Graphidium strigosum were rarely noted. The overall infection with nematodes did not differ between years. Similarly, as in the case of Eimeria older individuals were more often infected by nematodes. We observed some trends in parasite oocysts/eggs output; the protozoan oocysts were recorded more often in faecal samples collected in the evenings, whereas the nematodes eggs occurred frequently in the mornings. This situation may be related to the phenomenon of coprophagy occurring in the mammals of Lagomorpha order. The results of the study indicate that especially coccidiosis constitute permanently throughout the years an important problem in the rabbitry examined.

  11. Ketamine anaesthesia following premedication of rabbits with vitamin C.

    Science.gov (United States)

    Elsa, Abdullahi; Ubandawaki, Stephen

    2005-09-01

    The effects of vitamin C on ketamine anesthesia was studied. In normal rabbits the onset and duration of ketamine induced anesthesia were 6.0 +/- 0.5 and 36.0 +/- 0.9 min, respectively. Pre-treatment of rabbits with 30, 60 and 240 mg/kg, i.m. of vitamin C followed by ketamine 40 mg/ kg i.m. resulted in significant (p vitamin C and ketamine combinations. Serum analysis showed a significant (p vitamin C. These results suggest that vitamin C at higher doses could potentiate ketamine anesthesia in rabbits.

  12. Pancreas tumor model in rabbit imaged by perfusion CT scans

    Science.gov (United States)

    Gunn, Jason; Tichauer, Kenneth; Moodie, Karen; Kane, Susan; Hoopes, Jack; Stewart, Errol E.; Hadway, Jennifer; Lee, Ting-Yim; Pereira, Stephen P.; Pogue, Brian W.

    2013-03-01

    The goal of this work was to develop and validate a pancreas tumor animal model to investigate the relationship between photodynamic therapy (PDT) effectiveness and photosensitizer drug delivery. More specifically, this work lays the foundation for investigating the utility of dynamic contrast enhanced blood perfusion imaging to be used to inform subsequent PDT. A VX2 carcinoma rabbit cell line was grown in the tail of the pancreas of three New Zealand White rabbits and approximately 3-4 weeks after implantation the rabbits were imaged on a CT scanner using a contrast enhanced perfusion protocol, providing parametric maps of blood flow, blood volume, mean transit time, and vascular permeability surface area product.

  13. Particulate matter concentrations and emissions in rabbit farms

    Directory of Open Access Journals (Sweden)

    Elisa Adell

    2012-04-01

    Full Text Available The extent of the potential health hazards of particulate matter (PM inside rabbit farms and the magnitude of emission levels to the outside environment are still unknown, as data on PM concentrations and emissions in and from such buildings is scarce.  The purpose of this study was to quantify airborne PM10 and PM2.5 concentrations and emissions on two rabbit farms in Mediterranean conditions and identify the main factors related with farm activities influencing PM generation.  Concentrations of PM10 and PM2.5 were determined continuously using a tapered element oscillating microbalance (TEOM in one farm with fattening rabbits and one reproductive doe farm in autumn.  At the same time as PM sampling, the time and type of human farm activity being performed was recorded. Additionally, temperature, relative humidity and ventilation rate were recorded continuously.  Emissions were calculated using a mass balance on each farm.  Results showed PM concentrations in rabbit farms are low compared with poultry and pig farms.  Average PM10 concentrations were 0.082±0.059 mg/m3 (fattening rabbits, and 0.048 ±0.058 mg/m3 (reproductive does. Average PM2.5 concentrations were 0.012±0.016 mg/m3 (fattening rabbits, and 0.012±0.035 mg/m3 (reproductive does. Particulate matter concentrations were significantly influenced by the type of human farm activity carried out in the building rather than by animal activity.  The main PM-generating activity on the fattening rabbit farm was sweeping, and the major PM-generating activity in reproductive does was sweeping and burning hair from the cages.  Average PM10 emissions were 5.987±6.144 mg/place/day (fattening rabbits, and 14.9±31.5 mg/place/day (reproductive does.  Average PM2.5 emissions were 0.20±1.26 mg/place/day (fattening rabbits, and 2.83±19.54 mg/place/day (reproductive does.  Emission results indicate that rabbit farms can be considered relevant point sources of PM emissions, comparable to

  14. In vivo behavior of detergent-solubilized purified rabbit thrombomodulin on intravenous injection into rabbits

    Energy Technology Data Exchange (ETDEWEB)

    Ehrlich, H.J.; Esmon, N.L.; Bang, N.U. (Eli Lilly and Company, Indianapolis, IN (USA))

    1990-02-01

    Thrombomodulin is a thrombin endothelial cell membrane receptor. The thrombomodulin-thrombin complex rapidly activates protein C resulting in anticoagulant activity. We investigated the anticoagulant effects and pharmacokinetic behavior of detergent-solubilized purified rabbit thrombomodulin labeled with iodine 125 when intravenously injected into rabbits. Thrombomodulin half-life (t1/2) was determined by tracking the 125I-radiolabeled protein and the biologic activity as determined by the prolongation of the activated partial thromboplastin time (APTT) and thrombin clotting time (TCT). When 200 micrograms/kg 125I-thrombomodulin was injected into rabbits, the APTT and TCT were immediately prolonged, whereas no effect on the prothrombin time was seen. In vitro calibration curves enabled us to convert the prolongations of the clotting times into micrograms per milliliter thrombomodulin equivalents. The best fit (r greater than 0.99) for the disappearance curves was provided by a two-compartment model with mean t1/2 alpha (distribution phase) of 18 minutes for 125I, 12 minutes for APTT, and 20 minutes for TCT, and mean t1/2 beta (elimination phase) of 385 minutes for 125I, 460 for APTT, and 179 for TCT. The administration of two doses of endotoxin (50 micrograms/kg) 24 hours apart did not accelerate the turnover rate of 125I-thrombomodulin as measured by the disappearance of 125I from the circulation. Thus, detergent-solubilized purified thrombomodulin administered intravenously circulates in a biologically active form for appreciable time periods.

  15. L-strip proximity fed ga

    Directory of Open Access Journals (Sweden)

    Ashish Singh

    2014-03-01

    Full Text Available In this article, the analysis of dualband L-strip fed compact semi-circular disk microstrip patch antenna has been presented using circuit theory concept. The antenna parameters such as return loss, VSWR and radiation pattern are calculated. The effect of geometric dimensions of the proposed antenna such as length of vertical and horizontal portion of L-strip is investigated. It is found that antenna resonate at two distinct modes i.e. 1.3 GHz and 6.13 GHz for lower and upper resonance frequencies respectively. The bandwidth of the proposed antenna at lower resonance frequency is 6.61% (simulated and 10.64% (theoretical whereas at upper resonance frequency, it is 6.02% (simulated and 9.06 % (theoretical. The theoretical results are compared with IE3D simulation results as well as experimental results and they are in close agreement.

  16. Pharmacokinetics of recombinant human basic fibroblast growth factor in rabbits and mice serum and rabbits aqueous humor

    Institute of Scientific and Technical Information of China (English)

    Qi ZHANG; Guang-ji WANG; Jian-guo SUN

    2004-01-01

    AIM: To study the pharmacokinetics of recombinant human basic fibroblast growth factor (rhbFGF) in rabbits and mice after iv and postocular administration, and the changes of rhbFGF in rabbits aqueous humor after postocular administration. METHODS: After iv or postocular administration three doses of rhbFGF in rabbits and mice,rhbFGF concentration in serum and rabbit aqueous humor was determined by enzyme-linked immunosorbent .assay. RESULTS: Serum concentration-time data of rabbits after iv administration of rhbFGF 1, 2, and 4 μg/kg were fitted to bi-exponential equations with half-lives of 0.9, 0.9, and 0.6 min for T1/2α and 7, 8, and 4.7 min for T1/2β.Plasma concentration-time data of mice after iv administration of rhbFGF 2.5, 5 and 10 μg/kg were fitted to biexponential equations with half-lives of 0.4, 0.6, and 0.9 min for T1/2α and 6, 5, and 7 min for T1/2β. The AUCs were linearly correlated to doses in both cases (rrabbit=0.997, rmouse=0.999). The serum concentrations of rhbFGF were very low, near to the background after postocular administration of 2 or 5 μg/kg, in both rabbits and mice. The rhbFGF levels in rabbits aqueous humor were higher than control 8 h postdose (P<0.01). CONCLUSION: rhbFGF within the examined doses had a linear pharmacokinetics in rabbits and mice. High concentration of rhbFGF was found in rabbits aqueous humor after postocular administration.

  17. Effects of Naoxintong on atherosclerosis and inducible nitric oxide synthase expression in atherosclerotic rabbit

    Institute of Scientific and Technical Information of China (English)

    ZHONG Xiao-nan; WANG Hong-hao; LU Zheng-qi; DAI Yong-qiang; HUANG Jian-hua; QIU Wei; SHU Ya-qing

    2013-01-01

    Background High levels of nitric oxide (NO) produced by inducible NO synthase (iNOS) have been associated with atherosclerosis processes.Naoxintong is a traditional Chinese medicine for treatment of cerebrovascular and cardiovascular disease.The aim of the present study was to detect and quantify changes of iNOS mRNA and NO levels in the vessel wall after the administration of Naoxintong in an atherosclerotic rabbit model.Methods Forty New Zealand white rabbits were randomly divided into five groups (n=8).Rabbits were fed a standard diet (group A),an atherogenic diet consisting of 79% standard feed+1% cholesterol+5% lard+15% egg yolk powder (group B),an atherogenic diet with Naoxintong 0.25 mg·kg-1·d-1 (group C),an atherogenic diet with Naoxintong 0.5mg·kg-1·d-1 (group D),or atherogenic diet with Naoxintong 1.0 mg·kg-1·d-1 (group E) for 12 weeks.Results Supplemented administration of Naoxintong led to a down-regulation of cholesterol (CHOL) (P <0.001) and low-density lipoprotein (LDL) (P <0.001).The trend became more notable as the dose of Naoxintong increased; group Cvs.group B (CHOL,P=0.568; LDL-cholesterol (LDL-C),P=0.119),group D vs.group B (CHOL,P=0.264; LDL-C,P=0.027),group E vs.group B (CHOL,P=0.028; LDL-C,P=0.002).Atherosclerotic lesions in aorta were reduced in Naoxintong groups (groups C,D,E) compared to group B.Group B had higher iNOS mRNA (P=0.001) and NO level (P<0.001) than group A.Compared with the atherogenic diet fed-rabbits,Naoxintong supplements decreased the expression of iNOS mRNA (P <0.001) and the NO level (P <0.001) in the vessel wall.Groups given a higher Naoxintong dose exhibited greater benefits.iNOS mRNA and NO levels seemed to be reduced in group C,although the difference did not quite reach statistical significance (iNOS mRNA,P=0.130; NO,P=0.038).iNOS mRNA and NO levels significantly decreased in group D (iNOS mRNA,P=0.019; NO,P=0.018) and group E (iNOS mRNA,P=0.004; NO,P<0.001).Conclusion Naoxintong has

  18. Rabbit hepatitis E virus is an opportunistic pathogen in specific-pathogen-free rabbits with the capability of cross-species transmission.

    Science.gov (United States)

    Liu, Baoyuan; Sun, Yani; Du, Taofeng; Chen, Yiyang; Wang, Xinjie; Huang, Baicheng; Li, Huixia; Nan, Yuchen; Xiao, Shuqi; Zhang, Gaiping; Hiscox, Julian A; Zhou, En-Min; Zhao, Qin

    2017-03-01

    Hepatitis E virus (HEV) has been detected in rabbits, a recently identified natural reservoir. In this study, anti-HEV antibodies and viral RNA were detected in rabbits sourced from a specific-pathogen-free (SPF) rabbit vendor in Shaanxi Province, China. BLAST results of partial HEV ORF2 genes cloned here indicated that two viral strains circulated in the rabbits. Sequence determination of the complete genome (7302bp) of one strain and a partial ORF1 gene (1537bp) of the other strain showed that they shared 90% identity with one another and 78%-94% identity with other known rabbit HEVs. In addition, inoculation with rabbit HEV from SPF rabbits studied here resulted in infection of SPF pigs; this cross-species transmission was evidenced by seroconversion, viremia and faecal virus shedding. These results suggest that to prevent spread of this zoonotic pathogen, rabbits should be tested routinely for HEV RNA in SPF vendor facilities.

  19. Electrocardiogram of rabbits experimentally intoxicated with carbaryl.

    Science.gov (United States)

    Kossakowski, S

    1987-01-01

    Experiments were carried out on 24 rabbits intoxicated intragastrically with carbaryl in single doses of 500, 750, 1000 and 1250 mg/kg. Electrocardiograms (ECGs) were performed in 3 limb leads before and 1, 2, 4, 6h and 1, 2, 4, 6, 14d after intoxication and in a final stage of lethal intoxications--in a continuous manner. It has been found that, depending on the intensification of disease, ECG changes were characterized by the decreased heart rate with stimulations coming from the left ventricle, premature supraventricular stimulations, increased T wave amplitude and its reversion. These changes, except two lethal cases (1250 mg/kg), were compensated but the compensatory heart abilities decreased with increasing carbaryl doses.

  20. Autoradiographic studies in a rabbit osteoarthrosis model

    Energy Technology Data Exchange (ETDEWEB)

    Fengler, H.; Franz, R. (Medizinische Akademie, Dresden (German Democratic Republic))

    1982-02-01

    To study the onset of the osteoarthrotic process, an osteoarthrosis model was used on the knee joint in adult rabbits by a valgus deformity of the proximal tibia of 30/sup 0/ in conformity with Reimann 1973. The synthesis capacity of the chondrocytes was investigated by using /sup 35/S-sulfate autoradiographies. Already prior to the affection of the superficial integrity of the cartilage it was possible to observe an enhanced glycosaminoglycan synthesis, but with progressing fibrillation the sulfate incorporation was found to be diminished. Thanks to autoradiography with /sup 3/H-thymidine the replication of the chondrocytes was already found at very early stages of osteoarthrosis that is likely to occur mitotically. The osteotomy itself induces mitoses and an enhanced glycosaminoglycan synthesis.

  1. Tricolemoma em coelho Tricholemmoma in a rabbit

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    Krishna Duro de Oliveira

    1999-06-01

    Full Text Available Tricolemoma é um raro tumor benigno, composto por células epiteliais claras derivadas da bainha externa da raiz do folículo piloso. Esses tumores têm sido descritos no homem e raramente em cães. No presente relato, descreve-se a ocorrência de um tricolemoma, de 1cm de diâmetro em um coelho adulto, cujas características histológicas são muito semelhantes às descritas para esses tumores em cães.Tricholemmoma is a rare benign tumor composed of clear epithelial cells derived from the outer root sheath of the hair follicle. The tumor has been described in man and rarely in dogs. This report deals with the occurrence of a tricholemmoma , with a diemeter of 1cm, in an adult rabbit. The histologic features are similar to those described in such tumors in dogs.

  2. Effect of diet supplementation with live yeast Saccharomyces cerevisiae on growth performance, caecal ecosystem and health of growing rabbits

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    T. Belhassen

    2016-09-01

    Full Text Available The aim of this study was to determine the effect of the live yeast Saccharomyces cerevisiae on the growth performance, caecal ecosystem and overall health of growing rabbits. A control diet was formulated (crude protein: 15.9%; neutral detergent fibre: 31.6% and another diet obtained by supplementing the control diet with 1 g of Saccharomyces cerevisiae (6.5×109 colony-forming units per kg of diet. Ninety 35-d old rabbits were allotted into 3 groups: TT (rabbits offered the supplemented diet from 17 d of age onwards, CT (rabbits offered supplemented diet from 35 d and CC (rabbits fed non-supplemented diet. Body weight (BW and feed intake were measured weekly and mortality was controlled daily. At 35, 42 and 77 d of age, 6 rabbits from each group were slaughtered and digestive physiological traits, serum clinical chemistry parameters, fermentation traits, and the composition of caecal microbiota examined. At 42 and 56 d of age, 10 rabbits from each group were injected intraperitoneally with 100 μg/animal of ovalbumin and blood samples were collected for examination of plasma immunological parameters. Throughout the experiment (5-11 wk, weight gain and feed intake (37.8 and 112.6 g/d, on av. were not affected by yeast, except for weight gain in the first week after weaning, which was the highest in TT animals among the 3 groups (48.1 vs. 43.9 and 44.2 g/d for TT, CC and CT, respectively; P=0.012. This may be due to the increased trend in feed intake (P=0.072 in the TT group (96.4 g/d compared to the others. Mortality (5/90 was low and did not differ among the 3 groups. Treatments had no effect on slaughter traits at the 3 sampling dates (35, 42 and 77 d. Only the weight of the empty caecum (% BW was higher (P=0.02 in CC (2.2% and CT (2.3% than in TT group (1.8% at 77 d of age. Treatments did not overtly affect the caecal microbiota, although the number of total anaerobic bacteria and Bacteroides were lower (108 and 107/g caecal digesta

  3. Experimentos com a polpa cítrica em ovinos e coelhos Experiments with citrus pulp in sheep and rabbits

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    Carlos Hubinger Tokarnia

    2001-12-01

    Full Text Available Ovinos e coelhos receberam em sua alimentação polpa cítrica em quantidades elevadas durante períodos prolongados, com a finalidade de estabelecer um modelo experimental. A polpa cítrica para os experimentos realizados nos ovinos era a mesma usada por criador do município de Lorena, SP, que perdeu 5 de um total de 56 vacas em lactação, em um período de poucas semanas; a doença caracterizava-se histologicamente por lesões granulomatosas. Os 4 ovinos usados nos experimentos receberam 800g/dia/animal desta polpa cítrica durante 10 a 11 meses. Nenhum dos ovinos mostrou o quadro clínico-patológico da intoxicação pela polpa cítrica observado nos bovinos ou outras alterações. Nos experimentos com os coelhos foi usada polpa cítrica de duas procedências. Na primeira série de experimentos com 6 coelhos foi usada a mesma polpa cítrica dos experimentos com os ovinos; na segunda série de experimentos com mais 6 coelhos utilizou-se a polpa cítrica que causou lesões granulo-matosas em cães. Os coelhos da primeira série ingeriram em média 51,5 g/dia/animal, os da segunda série em média 35,2 g/dia/animal da polpa cítrica. Os coelhos foram sacrificados 2, 3 , 6 e 12 meses após o início dos experimentos. Nenhum dos coelhos mostrou o quadro clínico-patológico da intoxicação pela polpa cítrica ou outras alterações.Citrus pulp was fed to sheep and rabbits in high doses and during long periods, in order to find an experimental model. The citrus pulp used in the experiments in sheep was the same used by a cattle breeder in Lorena, State of São Paulo, who lost out of a total of 56 lactating cows during a period of a few weeks 5 animals by a disease characterized histologically by granulomatous lesions. The 4 sheep used in the experiments received 800g/day/animal of this citrus pulp during 10 to 11 months. They were euthanized at the end of the experiment. None of the sheep showed the clinical-pathological picture of citrus pulp

  4. Pharmacokinetics of topically applied sparfloxacin in rabbits

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    Satia Milan

    2005-01-01

    Full Text Available PURPOSE: Fluoroquinolones are antimicrobial agents that have a broad spectrum of activity and are widely used against many of the ocular pathogens, responsible for conjunctivitis, blepharitis, corneal ulcers etc. The aim of our study was to evaluate the ocular pharmacokinetics of sparfloxacin (0.3% w/v in the aqueous humour of rabbits. MATERIALS AND METHODS: Pharmacokinetics of topically administered sparfloxacin were determined after a single application of 50 µl topically. The aqueous humour samples were collected at 0, 0.25, 0.5, 1, 2, 3, 4, 5 or 6 hours after instillation. High Performance Thin Layer Chromatographic method was used to analyse the drug concentration in the aqueous humour samples. RESULTS: Fifteen minutes after the instillation of 50 µl of sparfloxacin 0.3% solution, the mean concentration in aqueous humour was found to be 1.4 µg/ml, which reaches the peak level of 3.7 µg/ml after 1.3 hours. At 6 hours, the sparfloxacin aqueous levels were 0.562 µg/ml. The clinical efficacy was predicted based on the Maximum Concentration (Cmax: Minimum Inhibitory Concentration (MIC and Area Under the Concentration-time curve (AUC:MIC ratios. CONCLUSION: The sparfloxacin levels in aqueous humour of rabbits are sufficiently high up to the 6 hours after instillation in the conjunctival sac to provide bactericidal effect against most of the ocular pathogens. Both Cmax:MIC and AUC:MIC ratios are high enough to provide bactericidal effect against most of the ocular pathogens. Sparfloxacin (0.3% ophthalmic preparation has excellent penetration through cornea.

  5. Acute effects of vinegar intake on some biochemical risk factors of atherosclerosis in hypercholesterolemic rabbits

    Science.gov (United States)

    2010-01-01

    Background Exaggerated postprandial spikes in blood glucose and lipids induce proportional increases in oxidative stress, which acutely trigger impairment endothelial, inflammation and increased risk of future cardiovascular events. In this research, we have investigated acute effects of vinegar intake on some of the biochemical atherosclerosis risk factors in high cholesterol fed rabbits to see if we can find a probable protective value for it. Methods The rabbits were randomly divided into four groups: normal diet, high cholesterol diet (%1cholesterol), %1 cholesterol with 5 ml vinegar (low dose), %1 cholesterol with 10 ml vinegar (high dose). After fasting for 12-15 hours, blood samples were taken to determine baseline values. Three hours after feeding, blood samples were collected again to investigate acute effects of vinegar intake on the measured factors. Results Using high-dose vinegar with cholesterolemic diet caused significant reduce in LDL-cholesterol (LDL-C), oxidized-LDL (ox-LDL), malondialdehyde (MDA), total cholesterol (TC) and apolipoprotein B (ApoB) in comparison with hypercholesterolemic diet. Consumption low-dose vinegar with cholesterolemic diet induced a significant decrease in fibrinogen and glucose compared to hypercholesterolemic diet. Level of serum nitrite, nitrate, triacylglycerol (TAG), HDL-cholesterol (HDL-C), apolipoprotein A (ApoA), serum glutamic pyruvic transaminase (SGPT), serum glutamic oxaloacetate transaminase (SGOT) and C-reactive protein (CRP) were not significantly difference in low and high doses vinegar with cholesterolemic diet compared to hypercholesterolemic diet. A significant difference was observed for LDL-C, ApoB100 and TC between low and high doses vinegar. Conclusion This study suggest that vinegar, might have some acute effects on biochemical risk factors of atherosclerosis and a probable protective value can be considered for its postprandial use. PMID:20109192

  6. Acute effects of vinegar intake on some biochemical risk factors of atherosclerosis in hypercholesterolemic rabbits

    Directory of Open Access Journals (Sweden)

    rohani Ali

    2010-01-01

    Full Text Available Abstract Background Exaggerated postprandial spikes in blood glucose and lipids induce proportional increases in oxidative stress, which acutely trigger impairment endothelial, inflammation and increased risk of future cardiovascular events. In this research, we have investigated acute effects of vinegar intake on some of the biochemical atherosclerosis risk factors in high cholesterol fed rabbits to see if we can find a probable protective value for it. Methods The rabbits were randomly divided into four groups: normal diet, high cholesterol diet (%1cholesterol, %1 cholesterol with 5 ml vinegar (low dose, %1 cholesterol with 10 ml vinegar (high dose. After fasting for 12-15 hours, blood samples were taken to determine baseline values. Three hours after feeding, blood samples were collected again to investigate acute effects of vinegar intake on the measured factors. Results Using high-dose vinegar with cholesterolemic diet caused significant reduce in LDL-cholesterol (LDL-C, oxidized-LDL (ox-LDL, malondialdehyde (MDA, total cholesterol (TC and apolipoprotein B (ApoB in comparison with hypercholesterolemic diet. Consumption low-dose vinegar with cholesterolemic diet induced a significant decrease in fibrinogen and glucose compared to hypercholesterolemic diet. Level of serum nitrite, nitrate, triacylglycerol (TAG, HDL-cholesterol (HDL-C, apolipoprotein A (ApoA, serum glutamic pyruvic transaminase (SGPT, serum glutamic oxaloacetate transaminase (SGOT and C-reactive protein (CRP were not significantly difference in low and high doses vinegar with cholesterolemic diet compared to hypercholesterolemic diet. A significant difference was observed for LDL-C, ApoB100 and TC between low and high doses vinegar. Conclusion This study suggest that vinegar, might have some acute effects on biochemical risk factors of atherosclerosis and a probable protective value can be considered for its postprandial use.

  7. THE EFFECT OF DIETARY SUPPLEMENTATION OF HERBAL EXTRACTS ON GROWTH PERFORMANCE AND HEALTH STATUS OF RABBITS

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    Mária Chrenková

    2013-02-01

    Full Text Available Seventy two rabbits (Hycole, (♂+♀ 5 weeks old were divided into 2 experimental (EG and one control (CG group. The feed mixture A in control (CG did not contain any coccidiostat. In the first experimental group (EG1 was tested a complete granulated mixture B enriched with 30 g dry extract Eleutherococcus senticosus per 100 kg mixture. The animals were fed mixture B in combination with probiotic strain Enterococcus faecium EF AL 41 applied orally at a dose (10 9 CFU. ml-1 500 μl per animal in drinking water during 21 days. In the second group (EG2 was tested complete granulated mixture B. The experiment lasted 42 days. Body weight and feed consumption were registered weekly. Samples of faeces were taken at days 1, 21 and 42 to monitor the counts and effect of natural substances. Blood samples were also taken to check biochemical parameters using commercial kits. The samples for microbiological analyses were treated by the standard microbiological method according to ISO with appropriate media. Results presented in this study introduce different mechanisms of action for different classes of feed additives and how these may influence the observed live performance benefits. Application of Eleutherococcus extract in combination with EF AL 41 strain in rabbits was beneficial for performance. Additives reduced counts of S. aureus and Clostridium-like sp in faeces. Bioactive substances can be beneficial for animal health as was repeatedly confirmed by our results (mortality in EG1- 12%, EG2 - 8% compared to CG- 17%.

  8. Artificial feeding synchronizes behavioral, hormonal, metabolic and neural parameters in mother-deprived neonatal rabbit pups.

    Science.gov (United States)

    Morgado, Elvira; Juárez, Claudia; Melo, Angel I; Domínguez, Belisario; Lehman, Michael N; Escobar, Carolina; Meza, Enrique; Caba, Mario

    2011-12-01

    Nursing in the rabbit is under circadian control, and pups have a daily anticipatory behavioral arousal synchronized to this unique event, but it is not known which signal is the main entraining cue. In the present study, we hypothesized that food is the main entraining signal. Using mother-deprived pups, we tested the effects of artificial feeding on the synchronization of locomotor behavior, plasma glucose, corticosterone, c-Fos (FOS) and PERIOD1 (PER1) rhythms in suprachiasmatic, supraoptic, paraventricular and tuberomammillary nuclei. At postnatal day 1, an intragastric tube was placed by gastrostomy. The next day and for the rest of the experiment, pups were fed with a milk formula through the cannula at either 02:00 h or 10:00 h [feeding time = zeitgeber time (ZT)0]. At postnatal days 5-7, pups exhibited behavioral arousal, with a significant increase in locomotor behavior 60 min before feeding. Glucose levels increased after feeding, peaking at ZT4-ZT12 and then declining. Corticosterone levels were highest around the time of feeding, and then decreased to trough concentrations at ZT12-ZT16, increasing again in anticipation of the next feeding bout. In the brain, the suprachiasmatic nucleus had a rhythm of FOS and PER1 that was not significantly affected by the feeding schedule. Conversely, the supraoptic, paraventricular and tuberomammillary nuclei had rhythms of both FOS and PER1 induced by the time of scheduled feeding. We conclude that the nursing rabbit pup is a natural model of food entrainment, as food, in this case milk formula, is a strong synchronizing signal for behavioral, hormonal, metabolic and neural parameters. © 2011 The Authors. European Journal of Neuroscience © 2011 Federation of European Neuroscience Societies and Blackwell Publishing Ltd.

  9. Effective protection of rabbits' explosive brain injury through blocking ...

    African Journals Online (AJOL)

    Effective protection of rabbits' explosive brain injury through blocking gap junction communication. ... Log in or Register to get access to full text downloads. ... an important role in spreading of apoptotic and necrotic signals from injured and ...

  10. Effective protection of rabbits' explosive brain injury through blocking ...

    African Journals Online (AJOL)

    Effective protection of rabbits' explosive brain injury through blocking gap junction communication. ... If you would like more information about how to print, save, and work with PDFs, Highwire Press provides a helpful Frequently Asked ...

  11. The role of rabbit meat as functional food.

    Science.gov (United States)

    Dalle Zotte, Antonella; Szendro, Zsolt

    2011-07-01

    Increasing consumer knowledge of the link between diet and health has raised the awareness and demand for functional food ingredients. Meat and its derivatives may be considered functional foods to the extent that they contain numerous compounds thought to be functional. This review will attempt to outline the excellent nutritional and dietetic properties of rabbit meat and offer an overview of the studies performed on the strategies adopted to improve the functional value of rabbit meat. Dietary manipulation has been seen to be very effective in increasing the levels of essential FA, EPA, DHA, CLA, branched chain FA, vitamin E, and selenium in rabbit meat. Dietary fortification with vitamin E or natural products such as oregano essential oil, chia seed oil, and Spirulina platensis microalga seem promising in improving the oxidative stability of rabbit meat while also adding functional ingredients.

  12. A test of Darwin's ‘lop‐eared’ rabbit hypothesis

    National Research Council Canada - National Science Library

    Cordero, G. A; Berns, C. M

    2016-01-01

    ... other. Darwin provided a striking example of this process in the ‘lop‐eared’ rabbit by demonstrating that artificial selection for long external ears induced variation in the external auditory meatus...

  13. Study on impression smears of hepatic coccidiosis in rabbits.

    Science.gov (United States)

    Sivajothi, S; Reddy, B Sudhakara; Rayulu, V C

    2016-09-01

    Hepatic coccidiosis is a contagious and lethal disease condition in rabbits. The disease was recorded in six rabbits suffering with watery diarrhoea. Clinically, affected rabbits showed decreased growth rate, anorexia, debilitation, diarrhea and rough hair coat. Examination of the faecal samples revealed the presence of unsporulated oocysts of Eimeria spp. After sporulation Eimeria stiedae oocysts were identified. Postmortem examination revealed hepatomegaly with presence of discrete yellowish-white nodules on the surface of the liver. Impression smears from the liver revealed the presence of numerous developmental stages of E. stiedae corresponding with the stage of the liver lesion and also represent the histological changes of the liver. Rabbits were treated with a combination of sulphaquinoxaline and diaveridine for five days.

  14. The Complement-Fixation Test in Hepatic Coccidiosis of Rabbits

    Science.gov (United States)

    Rose, M. Elaine

    1961-01-01

    Antibodies to Eimeria stiedae were measured in rabbit serum by complement fixation. The titre rose to a maximum at about the 22nd day after infection, remained at this level for about 20 days and then declined. Antibodies were still detectable up to 160 days after infection. Evidence of past or present slight E. stiedae infection was found in clinically normal rabbits whose sera fixed complement with E. stiedae antigens. Challenge of rabbits which had recovered from a near-fatal infection had no effect upon the complement fixation titres of their sera. The serum of a rabbit which had been injected with alum-precipitated antigen fixed complement with E. stiedae antigens. However, the animal was still susceptible to a superimposed oral infection which had the effect of further increasing the serum titre. PMID:14493840

  15. THERAPEUTIC MANAGEMENT OF SARCOPTIC MANGE IN RABBIT WITH IVERMECTIN

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    Joyjit Mitra

    2014-06-01

    Full Text Available Sarcoptic mange infected non-descriptive rabbits were successfully treated with Ivermectin @ 400 µg / kg body weight sub-cutaneously once weekly for 4 weeks resulted complete recovery within a month in Kalyani area, West Bengal, India.

  16. ACUTE DERMAL TOXICITY STUDIES OF TROISTM IN NEWZEALAND WHITE RABBITS

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    Anurag Payasi

    2010-06-01

    Full Text Available The study was performed to assess the acute dermal toxicity of TroisTM in Newzealand white rabbit. Test substance was applied as such to the shaven skin of group of rabbits at the dose of 2000 mg/Kg body weight. Control group of animals were similarly treated but only with base. Following dosing up to 14 days the rabbits were observed for mortality and clinical sign of toxicity. No visible signs of toxicity after treatment were observed on the animals of both control and treated animals up to 14 days. Various haematological and biochemical parameters were evaluated and found to be in the normal limit, which indicates that no sign of toxicity in NewZealand white rabbits after 14 days treatment in respect to control group, proving safety of TroisTM in topical application.

  17. Bottom's Semiology: The Duck-Rabbit and Magritte's Pipe.

    Science.gov (United States)

    Berthoff, Ann E.

    1993-01-01

    Explores how a dyadic understanding of perception cancels the validity it might have as a model for the linguistic process. Discusses commonly misunderstood exhibits in the gallery of perception studies--the duck-rabbit and Magritte's pipe. (RS)

  18. Photodynamic therapy for implanted VX2 tumor in rabbit brains

    Science.gov (United States)

    Li, Fei; Feng, Hua; Lin, Jiangkai; Zhu, Gang; Chen, Zhi; Li, Cong-yan

    2005-07-01

    To evaluate the therapeutic effect and the safety of single photodynamic therapy (PDT) with hematoporphyrin derivative produced in China, 60 New Zealand adult rabbits with VX2 tumor implanted into the brain were divided randomly into non-PDT-group and PDT-group. 36 rabbits of the PDT-group were performed photodynamic therapy. The survival time, neurological deteriorations, intracranial pressure (ICP), histology, pathology, tumor volume and brain water content were measured. Other 12 rabbits were received hematoporphyrin derivative and light irradiation of the normal brain. The ICP, histology, pathology, and brain water content were measured. The result indicated that Simple PDT may elongate the average survival time of the rabbits with VX2 tumors significantly; kill tumor cells; cause transient brain edema and increase ICP, but it is safe to be used in treating brain tumor.

  19. Effect of monocular deprivation on rabbit neural retinal cell densities

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    Philip Maseghe Mwachaka

    2015-01-01

    Conclusion: In this rabbit model, monocular deprivation resulted in activity-dependent changes in cell densities of the neural retina in favour of the non-deprived eye along with reduced cell densities in the deprived eye.

  20. Development of an Acetate-Fed or Sugar-Fed Microbial Power Generator for Military Bases

    Science.gov (United States)

    2011-01-01

    Sucrose • Acetate Propionate • Hydrogen gas • Lactate • Formate • !so-butyrate Unknown fbiomass -’-soluble products ) KM. 80 60 I" 20 DavO...lactate and acetate as fermentation products and is ready for test in the MFC modules. 1. Anode materials We compared the performances of acetate-fed...microbial electrolysis cells (MECs) with graphite rods and stainless steel meshes as anodes to select the optimum material for use in MFC modules to