WorldWideScience

Sample records for volunteer blood donors

  1. Evaluation of the return rate of volunteer blood donors

    Directory of Open Access Journals (Sweden)

    Adriana de Fátima Lourençon

    2011-06-01

    Full Text Available BACKGROUND: To convert first-time blood donors into regular volunteer donors is a challenge to transfusion services. OBJECTIVES: This study aims to estimate the return rate of first time donors of the Ribeirão Preto Blood Center and of other blood centers in its coverage region. METHODS: The histories of 115,553 volunteer donors between 1996 and 2005 were analyzed. Statistical analysis was based on a parametric long-term survival model that allows an estimation of the proportion of donors who never return for further donations. RESULTS: Only 40% of individuals return within one year after the first donation and 53% return within two years. It is estimated that 30% never return to donate. Higher return rates were observed among Black donors. No significant difference was found in non-return rates regarding gender, blood type, Rh blood group and blood collection unit. CONCLUSIONS: The low percentage of first-time donors who return for further blood donation reinforces the need for marketing actions and strategies aimed at increasing the return rates.

  2. Prevalence and Incidence of Syphilis among Volunteer Blood Donors in Israel

    Directory of Open Access Journals (Sweden)

    Leibovici Vera

    2014-01-01

    Full Text Available Data of 1,290,222 volunteer blood donors, in a 5-year period, was analyzed for prevalence and incidence of syphilis. Subsequent testing of donations positive in Treponema pallidum hemagglutination assay included Venereal Disease Research Laboratory and fluorescent Treponemal antibody absorption. Stepwise logistic regression model was used to identify positive syphilis serology. Prevalence of syphilis was 47 : 100,000, similar in men and women and increased significantly with age (P<0.001. Native Israelis had the lowest prevalence rate of syphilis (21 : 100,000, while a significantly higher prevalence was found among immigrants from Africa, Eastern Europe, and South America (odds ratios of 19.0, 10.8, and 7.3, resp., P<0.001 for each. About 33.2% of the seropositive donors had evidence of recent infection, and 66.8% had past infections. Incidence rate reached 8 : 100,000 person-years. Coinfection with HIV, HCV, and HBV was calculated as 8%, 1.88%, and 0.37% for positive donations, respectively. The data support the need to continue screening blood donors in Israel for syphilis and employ preventive measures to populations at risk, in order to improve public health, blood safety, and quality. A subsequent study to assess blood donors’ knowledge, attitude, and behavior is planned. In times of global migration this information may be useful to blood services worldwide.

  3. The Frequency of Hepatitis B and C Among Volunteer Blood Donors in Balochistan

    Directory of Open Access Journals (Sweden)

    Zafar Ahmad Khan

    2007-08-01

    Full Text Available Background and Aims: In this cross-sectional study, the frequency of hepatitis B and C among volunteer blood donors in blood banks of Sandeman Provincial & Lady Duffren Hospitals, Quetta, Pakistan was estimated.Methods: 1474 blood donors were selected by convenient sampling. After obtaining informed consent, brief history and examination were done. They were tested for HBsAg and anti-HCV antibodies in laboratory. The collected data analyzed by SPSS 12.0 and results shown in frequencies and percentages.Results: Out of 1474 blood donors, 1284 (87.1% were males and 190 (12.9% were females. The mean age was 25 (range: 16-49 years. 71 (4.8% were positive for HBsAg (63 males & 8 females, 26 (1.8% for anti-HCV (21 males & 5 females and 4 (0.27% were positive for both (3 males & 1 female. Most of them belonged to ages 21-40 years.Conclusions: The frequency of hepatitis B is same as that of other part of the country contrary to the belief that Balochistan is a high prevalent region for hepatitis B as compare to the rest of the country, while the frequency of hepatitis C is less than expected. Health education and vaccination for hepatitis B should be encouraged and more vigilant efforts should be done.

  4. Distribution of hepatitis C virus genotypes in volunteer blood donors from Chengdu, China.

    Science.gov (United States)

    Gong, Tianxiang; Zhao, Xin; Luo, Yijia; Hong, Ying; Li, Shuping; Fu, Xuemei

    2016-07-01

    Hepatitis C virus (HCV) is a significant pathogen of global concern. The virus is usually spread through blood contact, such as transfusion, hemodialysis and injection of illegal drugs. HCV genotypes have a geographic distribution in different areas. In this paper, we focus on the distribution of HCV genotypes from volunteer blood donors in Chengdu. The prevalence of genotypes was analyzed using phylogenetic analysis. Phylogenetic trees were constructed based on the HCV core and NS5B regions from 313 sequences. HCV sequences were classified into six subtypes, and HCV genotypes were determined with the following results: 1b in 283, 2a in 14, 3b in seven, 3a in three, 6a in five and 6u in one. Subtype 1b was the most common and accounted for approximately 90.41 % (283/313), and a virus of subtype 6u was isolated for the first time from the Chengdu area. Genotypes 4 and 5 were not detected.

  5. Attitude and opinion of volunteer blood donors regarding access to eugenic

    Directory of Open Access Journals (Sweden)

    Konstantina Chatzilaou

    2009-02-01

    Full Text Available PURPOSE: The assessment of the attitude and views of blood donors in relation to eugenics. As well as the relation of eugenics to several demographical factors such as educational level, sex age and religious beliefs.MATERIAL AND METHOD: The subjects of study were 252 blood donors, who voluntarily gave blood and its byproducts in a Blood Donation Center in Athens. The data was collected through an anonymous questionnaire especially designed for the purpose of this study.RESULTS: The overwhelming majority of volunteer blood-donors agree in percentage greater than 50%, that society will probably never offer sufficient support to individuals with disabilities and that a woman should be submitted to prenatal control to determine if she medically advised in relation to her age and family background. 79% agrees that they would want to learn more about the moral questions that arise from the application of genetics to human.Independent variables that influence the attitude towards eugenics were found to be the importance of religion, the level of education, the sex and the number of children.Also, there was a connection of religion and the number of children with the knowledge of moral questions on genetics.CONCLUSIONS: Prenatal control should be carried out only when serious genetic illnesses or illnesses whose treatment is imperative to start early are expected. The attitude of citizens towards individuals with disabilities should change radically so that the biases and social stereotypes are raised. A better provision of information of the public on the moral questions that derive from the application of genetics to humans is also suggested.

  6. Comparison of risk factors among blood donors, volunteers and replacement individuals, infected or not by hepatitis C virus

    Directory of Open Access Journals (Sweden)

    MJDB Felippe

    2009-01-01

    Full Text Available Hepatitis C is transmitted primarily parenterally by contaminated blood and is often associated with: intravenous drug abuse, invasive procedures, blood transfusions, acupuncture, tattooing, and alcohol and tobacco use. This study aimed to quantify and evaluate the risk factors among blood donors, volunteer blood donors and replacement individuals, infected or not by the C virus. The main transmission routes of C virus were identified in 55 men and 25 women (GI monitored by the Ambulatory Unit of the Department of Tropical Diseases, Botucatu Medical School, and in 24 men and 26 women (GII, all active blood donors at the Bauru State Hospital Transfusional Agency. Both groups were similar in: tobacco and alcohol consumption, sexual behavior, tattooing and illicit drug use. The duration of alcohol and tobacco consumption and blood transfusions in GI were longer, whereas the option for steady partners, condom use, disposable materials and piercings were predominant in GII. In conclusion, the risk factors for hepatitis C demonstrate the necessity of health policies that act on the primary and secondary prevention levels (respectively, reduction of infection incidence and hepatopathy risk.

  7. [The prevalence of hepatitis C antibodies among volunteer blood donors with elevated blood transaminase and antibodies to the B virus core antigen].

    Science.gov (United States)

    Gavilán Carrasco, J C; González Santos, P; Rosario Díaz, E

    1996-05-01

    The use of non-specific markers before 1989 (increased serum transaminase values and antibodies to hepatitis B core antigen) as a screening method for blood donors in an attempt to decrease the incidence of post-transfusional non-A non-B hepatitis (currently hepatitis C virus) was a matter of controversy. To determine the impact of the use of these markers on the detection of blood donors infected with hepatitis C virus, a prospective study was undertaken in Málaga (1988-1989) with 5,003 volunteer donors with two objectives: a) to know the prevalence of these non-specific markers (anti-HBc and increased serum transaminase) and antibodies to HCV (anti-C100) in our blood donor population; b) to determine whether the presence of some of these non specific markers in blood donors was associated with a higher rate of virus C infection. The prevalence of antibodies to HCV in blood donors with increased serum transaminase and/or anti-HBc was significantly higher than the prevalence found among the general blood donor population.

  8. "Seroprevalence of Cytomegalovirus, Hepatitis B, Hepatitis C and Human Immunodeficiency Virus Antibodies among Volunteer Blood Donors "

    Directory of Open Access Journals (Sweden)

    R Moniri

    2004-10-01

    Full Text Available The transfusion transmitted infections are potentially dangerous complications of transfusion therapy in immunocompromised patients. The aim of this study was to determine the prevalence of transmissible infections in blood donor population in Kashan, Iran. A total of 600 consecutive sera were tested for CMV-IgM antibody, HBsAg, hepatitis B core (HBc antibody, hepatitis C (HCV antibody, and HIV antibody with standard methods. Of the sera tested, 14 specimens (2.3% were CMV-IgM positive. The frequency of seropositive revealed no significant differences between male and female donors. The frequency rates of CMV-IgM seropositive tests tend to decline with increasing the age. There was no relation between the frequency rates of CMV-IgM seropositive with the educational level, socioeconomic status, marital status, urban dweller and rural resident patients. The prevalence of HBV, HCV, and HIV antibody was 0.5%, 0.5%, and 0%, respectively. These findings implied important clinical applications because detection of CMV positive sera may reduce the risk for transmission of CMV in blood transfusion and thereby decrease the risk on CMV-induced complications.

  9. Acute iritis induced by granulocyte colony-stimulating factor used for mobilization in a volunteer unrelated peripheral blood progenitor cell donor.

    Science.gov (United States)

    Parkkali, T; Volin, L; Sirén, M K; Ruutu, T

    1996-03-01

    We describe a volunteer unrelated peripheral blood progenitor cell donor with previously diagnosed dermatitis herpetiformis in whom the administration of G-CSF for the mobilization of precursor cells induced acute iritis. G-CSF has been administered to healthy people with minimal side-effects but when used in patients with autoimmune disorders worsening of symptoms or new manifestations may be a potential concern.

  10. An autoregressive integrated moving average model for short-term prediction of hepatitis C virus seropositivity among male volunteer blood donors in Karachi, Pakistan

    Institute of Scientific and Technical Information of China (English)

    Saeed Akhtar; Shafquat Rozi

    2009-01-01

    AIM: To identify the stochastic autoregressive integrated moving average (ARIMA) model for short term forecasting of hepatitis C virus (HCV) seropositivity among volunteer blood donors in Karachi, Pakistan. METHODS: Ninety-six months (1998-2005) data on volunteer blood donors tested at four major blood banks in Karachi, Pakistan were subjected to ARIMA modeling. Subsequently, a fitted ARIMA model was used to forecast HCV seropositive donors for 91-96 mo to contrast with observed series of the same months. To assess the forecast accuracy, the mean absolute error rate (%) between the observed and predicted HCV seroprevalence was calculated. Finally, a fitted ARIMA model was used for short-term forecasts beyond the observed series. RESULTS: The goodness-of-fit test of the optimum ARIMA (2,1,7) model showed non- s igni f icant autocorrelations in the residuals of the model. The forecasts by ARIMA for 91-96 mo closely followed the pattern of observed series for the same months, with mean monthly absolute forecast errors (%) over 6 mo of 6.5%. The short-term forecasts beyond the observed series adequately captured the pattern in the data and showed increasing tendency of HCV seropositivity with CONCLUSION: To curtail HCV spread, public health authorities need to educate communities and health care providers about HCV transmission routes based on known HCV epidemiology in Pakistan and its neighboring countries. Future research may focus on factors associated with hyperendemic levels of HCV infection.

  11. BLOOD DONORS CAMPAIGN

    CERN Multimedia

    2001-01-01

    A blood donors campaign, organized by the Centre de Transfusion Sanguine of Geneva will be held at CERN on Tuesday 13 March 2001 in restaurant nr 2, from 9.00 to 16.30 hrs If you already have a card giving your blood group, please bring this with you.

  12. BLOOD DONORS CAMPAIGN

    CERN Multimedia

    2001-01-01

    A blood donors campaign, organized by the Centre de Transfusion d'Annemasse will be held at CERN on Tuesday 14 November 2001 in restaurant nr 2, from 9.00 to 16.30 hrs If you already have a card giving your blood group, please bring this with you.

  13. BLOOD DONORS CAMPAIGN

    CERN Multimedia

    Medical Service

    2002-01-01

    Tuesday 19 March 2002 in restaurant nr 2, from 9.00 to 16.30 hrs A blood donors campaign, organized by the Centre de Transfusion sanguine of Geneva If you already have a card giving your blood group, please bring this with you.

  14. BLOOD DONORS CAMPAIGN

    CERN Document Server

    2000-01-01

    A blood donors campaign, organized by the Établissement de Transfusion de Rhône-Alpes will be held at CERN on Tuesday 14 November 2000 in restaurant nr 2, from 8.30 to 16.30 hrs If you already have a card giving your blood group, please bring this with you.

  15. BLOOD DONORS CAMPAIGN

    CERN Document Server

    2002-01-01

    Wednesday 13 November 2002 in restaurant nr 2, from 8.30 to 16.30 hrs will be held a blood donors campaign, organized by the Etablissement de Transfusion de Haute-Savoie If you already have a card giving your blood group, please bring this with you.

  16. Exposure and risk factors to coxiella burnetii, spotted fever group and typhus group Rickettsiae, and Bartonella henselae among volunteer blood donors in Namibia.

    Directory of Open Access Journals (Sweden)

    Bruce H Noden

    Full Text Available The role of pathogen-mediated febrile illness in sub-Saharan Africa is receiving more attention, especially in Southern Africa where four countries (including Namibia are actively working to eliminate malaria. With a high concentration of livestock and high rates of companion animal ownership, the influence of zoonotic bacterial diseases as causes of febrile illness in Namibia remains unknown.The aim of the study was to evaluate exposure to Coxiella burnetii, spotted fever and typhus group rickettsiae, and Bartonella henselae using IFA and ELISA (IgG in serum collected from 319 volunteer blood donors identified by the Blood Transfusion Service of Namibia (NAMBTS. Serum samples were linked to a basic questionnaire to identify possible risk factors. The majority of the participants (64.8% had extensive exposure to rural areas or farms. Results indicated a C. burnetii prevalence of 26.1% (screening titre 1∶16, and prevalence rates of 11.9% and 14.9% (screening titre 1∶100 for spotted fever group and typhus group rickettsiae, respectively. There was a significant spatial association between C. burnetii exposure and place of residence in southern Namibia (P0.012, especially cattle (P>0.006, were also significantly associated with C. burnetii exposure. Males were significantly more likely than females to have been exposed to spotted fever (P<0.013 and typhus (P<0.011 group rickettsiae. Three (2.9% samples were positive for B. henselae possibly indicating low levels of exposure to a pathogen never reported in Namibia.These results indicate that Namibians are exposed to pathogenic fever-causing bacteria, most of which have flea or tick vectors/reservoirs. The epidemiology of febrile illnesses in Namibia needs further evaluation in order to develop comprehensive local diagnostic and treatment algorithms.

  17. RESULTS OF THE SPECIAL BLOOD DONOR DAY

    CERN Multimedia

    SC Unit

    2008-01-01

    Responding to the HUG (Hôpitaux Universitaires de Genève) hospitals’ urgent appeal for blood donations during this summer season, the CERN medical staff organised a day of blood donations for the Swiss bloodbank CTS on 30 July. They were supported by NOVAE (Restaurant No. 1), who provided donors with a free snack. This specially arranged campaign was a success, as the 135 volunteers included 66 first-time donors, and a total of 99 standard bags of blood was collected. (Swiss hospitals need 1300 bags every day!) The CTS and CERN’s medical staff want to thank the donors and all others who helped make the event a success. Upcoming blood donor days at CERN: 12 November 2008 and 10 March 2009.

  18. RESULTS OF THE SPECIAL BLOOD DONOR DAY

    CERN Multimedia

    SC Unit

    2008-01-01

    Responding to the HUG (Hôpitaux Universitaires de Genève) hospitals’ urgent appeal for blood donations during this summer season, the CERN medical staff organised a day of blood donations for the Swiss bloodbank CTS on 30 July. They were supported by NOVAE (Restaurant No. 1), who provided donors with a free snack. This specially arranged campaign was a success, as the 135 volunteers included 66 first-time donors, and a total of 99 standard bags of blood were collected. (Swiss hospitals need 1300 bags every day!) The CTS and CERN’s medical staff wish to thank the donors and all others who helped make the event a success. Upcoming blood donor days at CERN: 12 November 2008 and 10 March 2009.

  19. Asymptomatic Malaria among Blood Donors in Benin City Nigeria.

    Directory of Open Access Journals (Sweden)

    Bankole Henry Oladeinde

    2014-09-01

    Full Text Available This study aimed at determining the prevalence and associated risk factors for asymptomatic malaria parasitaemia and anemia among blood donors in a private medical laboratory in Benin City, Nigeria.Venous blood was collected from a total of 247 blood donors. Malaria status, ABO, Rhesus blood groups and hemoglobin concentration of all participants were determined using standard methods.The prevalence of asymptomatic malaria infection was higher among commercial blood donors than volunteer group (commercial vs volunteer donor: 27.5 %vs. 13.8%; OR = 2.373, 95% CI = 0.793, 7.107, P = 0.174. Asymptomatic malaria was not significantly affected by gender (P = 0.733, age (P = 0.581, ABO (P = 0.433 and rhesus blood groups (P = 0.806 of blood donors. Age was observed to significantly (P = 0.015 affect malaria parasite density with donors within the age group of 21-26 years having the highest risk. The prevalence of anemia was significantly higher among commercial donors (commercial vs volunteer donors: 23.4% vs 3.4%: OR = 8.551, 95% CI = 1.135, 64.437, P = 0.013 and donors of blood group O type (P = < 0.0001.Asymptomatic malaria parasitaemia and anemia was higher among commercial donors than voluntary donors. Mandatory screening of blood donors for malaria parasite is advocated to curb transfusion transmitted malaria and associated sequelae.

  20. Iron deficiency in blood donors

    Directory of Open Access Journals (Sweden)

    Armando Cortés

    2005-03-01

    Full Text Available Context: Blood donation results in a substantial loss of iron (200 to 250 mg at each bleeding procedure (425 to 475 ml and subsequent mobilization of iron from body stores. Recent reports have shown that body iron reserves generally are small and iron depletion is more frequent in blood donors than in non-donors. Objective: The aim of this study was to evaluate the frequency of iron deficiency in blood donors and to establish the frequency of iron deficiency in blood donors according to sex, whether they were first-time or multi-time donors. Design: From march 20 to April 5, 2004, three hundred potential blood donors from Hemocentro del Café y Tolima Grande were studied. Diagnostic tests: Using a combination of biochemical measurements of iron status: serum ferritin (RIA, ANNAR and the hemoglobin pre and post-donation (HEMOCUE Vital technology medical . Results: The frequency of iron deficiency in potential blood donors was 5%, and blood donors accepted was 5.1%; in blood donors rejected for low hemoglobin the frequency of iron deficiency was 3.7% and accepted blood donors was 1.7% in male and 12.6% in female. The frequency of iron deficiency was higher in multi-time blood donors than in first-time blood donors, but not stadistic significative. Increase nivel accepted hemoglobina in 1 g/dl no incidence in male; in female increase of 0.5 g/dl low in 25% blood donors accepted with iron deficiency, but increased rejected innecesary in 16.6% and increased is 1 g/dl low blood donors female accepted in 58% (7/12, but increased the rejected innecesary in 35.6%. Conclusions: We conclude that blood donation not is a important factor for iron deficiency in blood donors. The high frequency of blood donors with iron deficiency found in this study suggests a need for a more accurate laboratory trial, as hemoglobin or hematocrit measurement alone is not sufficient for detecting and excluding blood donors with iron deficiency without anemia, and ajustes hacia

  1. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... Marrow Donor Program® (NMDP), a federally funded nonprofit organization that maintains an international registry of volunteers willing ... her German stem cell donor for the first time in Germany. #priceless - Duration: 1:04. Jacque Brohawn ...

  2. "Just" blood donors? A study on the multi-affiliations of blood donors.

    Science.gov (United States)

    Alfieri, Sara; Pozzi, Maura; Marta, Elena; Saturni, Vincenzo; Aresi, Giovanni; Guiddi, Paolo

    2017-07-25

    The present work proposes to explore a phenomenon well known in the world of blood donation, but little explored by literature: multi-affiliations. By that term, in this paper we mean blood donors' engagement in multiple associations of various natures (donation, recreation, sports, etc.) simultaneously. The first objective proposes to explore the phenomenon of multi-affiliations in descriptive terms; the second is to look into the differences-in terms of motivations, family-work-volunteerism reconciliation, life satisfaction, and membership satisfaction-between those who "only" carry out blood donation activity and those who instead participate in multiple associative realities concurrently. Participating in the research were 2674 donors from the Italian Association of Blood Donors (AVIS) (age range 18-65; 66.6% male) to which a self-report questionnaire was administered in the waiting rooms of numerous blood donation centers. Regarding the first objective, it emerged that only 35.9% of the participants "only" donate blood, while a good 64.1% is engaged also in other associations. Regarding the second objective, statistically significant differences emerge regarding many of the variables considered: social, values, ego-protection, and career motivation; capacity to reconcile family-volunteering and work-volunteering; life satisfaction; and membership satisfaction. The study offers precious information to the agencies that handle recruiting and retaining of donors. The agencies in fact can take away information on how to improve the multi-affiliations of their donors, an aspect that can facilitate their long-term retention. Copyright © 2017 Elsevier Ltd. All rights reserved.

  3. Blood donor: nursing care plan

    Directory of Open Access Journals (Sweden)

    Marco Antonio Zapata Sampedro

    2008-11-01

    Full Text Available The standardized nursing care plan can be used as a means through which the nurse will assess and identify the particular needs of the blood donor.To draw up the care plan, we have conducted the evaluation on the basis of the Marjory Gordon’s functional health patterns.The more prevailing diagnosis according to the NANDA taxonomy have been identified, results have been established according to the NOC (Nursing Outcomes Classification taxonomy, and nursing interventions have been suggested according to the NIC (Nursing Interventions Classification taxonomy. Also, certain potential complications, which are infrequent, must be observed and controlled in the blood donation process. Our main aim with this article has been to offer to professionals resources that grant to the caring activity scientific rigor, professional recognition and an unique and valid tool to evaluate the assistance with the best levels of quality for the blood donor.

  4. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... total__ Find out why Close Becoming a Blood Stem Cell Donor NCIcancertopics Loading... Unsubscribe from NCIcancertopics? Cancel Unsubscribe ... Ever considered becoming a bone marrow or blood stem cell donor? Follow this true story of a former ...

  5. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... total__ Find out why Close Becoming a Blood Stem Cell Donor NCIcancertopics Loading... Unsubscribe from NCIcancertopics? Cancel Unsubscribe ... Ever considered becoming a bone marrow or blood stem cell donor? Follow this true story of a former ...

  6. Becoming a Blood Stem Cell Donor

    Science.gov (United States)

    ... total__ Find out why Close Becoming a Blood Stem Cell Donor NCIcancertopics Loading... Unsubscribe from NCIcancertopics? Cancel Unsubscribe ... Ever considered becoming a bone marrow or blood stem cell donor? Follow this true story of a former ...

  7. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... Find out why Close Becoming a Blood Stem Cell Donor NCIcancertopics Loading... Unsubscribe from NCIcancertopics? Cancel Unsubscribe ... considered becoming a bone marrow or blood stem cell donor? Follow this true story of a former ...

  8. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... Find out why Close Becoming a Blood Stem Cell Donor NCIcancertopics Loading... Unsubscribe from NCIcancertopics? Cancel Unsubscribe ... considered becoming a bone marrow or blood stem cell donor? Follow this true story of a former ...

  9. Mutaciones del gen de la Hemocromatosis en donantes de sangre voluntarios y en pacientes con Porfiria cutánea tarda en Chile Mutations of hemochromatosis gene in volunteer blood donors and Chilean porphyria cutanea tarda patients

    Directory of Open Access Journals (Sweden)

    Carlos Wolff F

    2006-10-01

    studied in a PCT group of patients and compared with the frequency observed in a group of volunteer blood donors. PCT patients were cataloged as hereditary or acquired PCT carriers, whether or not they presented uroporphyrinogen decarboxilase gene mutations. Fifty percent of PCT patients were carriers of the disease's genetic type. Such percentage is significantly higher than what other authors have previously informed. H63D and C282Y mutations were present in 23% and 2.4% of the volunteer blood donors, respectively. Similar frequencies were informed by others authors in Chilean white ethnic populations, and also in Spaniard and Argentinean populations, but significantly higher than that observed in Chile's Araucanean aboriginal population. Probably the frequency of H63D and C283Y mutations are related to the Spaniard ascendancy dominance of Chile's white ethnic population. The frequency of HFE gene mutations in PCT patients was not different than what was observed in volunteer blood donors. Similarly, there was no statistical difference in the frequency of these mutations among patients with acquired or genetic PCT disease. With the obtained results, it is not possible postulate an association between PCT and the hereditary hemochromatosis of HFE gene mutations carrier conditions.

  10. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... on the use of BMT and PBSCT, see http://www.cancer.gov/cancertopics/fa... If you are ... registry of volunteers willing to be donors at http://www.marrow.org . Category Science & Technology License Standard ...

  11. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... on the use of BMT and PBSCT, see http://www.cancer.gov/cancertopics/fa... If you are ... registry of volunteers willing to be donors at http://www.marrow.org . Category Science & Technology License Standard ...

  12. Seroprevalence study on Helicobacter pylori infection among volunteer blood donors in Nanchang%南昌地区献血人群幽门螺杆菌感染的血清学分析

    Institute of Scientific and Technical Information of China (English)

    刘福昌; 傅颖媛; 陈火玲; 潘继红; 姜珍一; 许春玲; 王丽

    2013-01-01

    目的 调查南昌地区献血人群中幽门螺杆菌(Hp)感染的血清阳性率并对其影响因素进行分析.监控Hp在献血不同人群中感染变化规律,为优化献血人群作参考.方法 采用整群抽样方式抽取2010年6~9月南昌地区不同献血种类(全血和机采血小板)的献血者633人(男377人,女256人).采取统一调查表进行现场问卷调查方式,采用ELLSA检测血清中Hp-IgG抗体,数据统计分析采用SPSS 17.0软件.结果 献血人群总的Hp感染血清阳性率为48.66%(308/633).男女之间、不同ABO血型间Hp阳性率差异无统计学意义(P﹥0.05);不同年龄、婚姻状况、体重指数、医务人员与非医务人员间的Hp阳性率差异有统计学意义(P 0.05); the results of simple Logistic regression showed that factors with significant ORs were young age, unmarried, slim body, number of people living together, not smoking, eating irregularly and medical personnel (P < 0.05); the results of stepwise Logistic regression showed that factors entered regression model were number of people living together (OR = 1.394, X2=4.675, P = 0.031), eating regularly (OR = 0.650, X2= 10.902, P = 0.001), smoking (OR = 0.631, X2=6.432, P = 0.011), dedicate a blood platelets (OR = 0.731, X2=9.205, P= 0.002) and group AB (OR = 0.512, X2=3.995, P = 0.046). Conclusion Helicobacter pylori seroprevalence rates are high among the volunteer blood donors in Nanchang. Hp infection can be related to age, occupation, marital status, BMI, smoking, number of people living together and eating regulation. Blood group A and B is not a risk factor for acquiring Hp infection; the prevalence of anti-Hp IgG antibody of blood platelets dedication is lower compare to the whole blood donation. Hp seroprevalence in the distribution of blood donors provides a reference for improving the blood donation criteria and a reasonable choice of blood donors in Nanchang.

  13. Serological identification of D variants in volunteer blood donors in Nanchong region%南充地区无偿献血者D变异体血清学鉴定

    Institute of Scientific and Technical Information of China (English)

    杨松; 熊依军; 邹韬; 杨兴明; 黄文岳

    2015-01-01

    Objective To study the serological phenotype of D variants in volunteer blood donors initially-diagnosed Rh negative in Nanchong region of Sichuan province. Methods A total of 433 blood samples from 154 392 unrelated volunteer blood donors with Han nationality in Nanchong district of Sichuan province initially diagnosed as Rh negative were identified by indirect antiglobulin test for D variants ,and the negative results were subsequently detected by absorption-elution testing for DEL type from indirect antiglobulin testing. In addition ,all of the blood samples were screened and identified for irregular antibodies. Results Among 433 samples,402 cases(92.8%,402/433) were confirmed Rh negative and 23 cases(5.3%,23/433) were de-tected D variants,including DvccEe for 8 cases(34.8%,8/23),DvCcee for 6 cases(26.1%,6/23) and DvCCee for 3 cases(13.0%, 3/23);A total of 3 DEL phenotypes for 105 cases were found,including DelCcee for 87 cases(82.9%,87/105),DelCCee for 16 cases (15.2%,16/105),and DelCcEe for 2 cases(1.9%,2/105),all of which were associated with C antigen;Furthermore,irregular antibodies failed to be detected in plasma samples of donors with D variants and DEL type. Conclusion In the survey,serologi-cal phenotypes of D variant in volunteer blood donors initially-diagnosed Rh negative in Nanchong district of Sichuan province are learned,which has an important significance to guide the clinical blood transfusion and prevent hemolytic diseases of the newborns.%目的:研究四川省南充地区初检为Rh阴性无偿献血者中D变异体血清学表型。方法对433份初检为Rh阴性的血样(来自于2010年4月至2014年9月四川省南充地区无亲缘关系的154392名汉族无偿献血者)用间接抗球蛋白试验鉴定D变异体,再用吸收放散试验从间接抗球蛋白试验阴性的血样检出DEL型,对所有血样进行不规则抗体筛选鉴定。结果433份送检标本中共确认Rh阴性402例(92.8%,402/433),检出23

  14. Hemochromatosis: the new blood donor.

    Science.gov (United States)

    Leitman, Susan F

    2013-01-01

    Hereditary hemochromatosis (HH) due to homozygosity for the C282Y mutation in the HFE gene is a common inherited iron overload disorder in whites of northern European descent. Hepcidin deficiency, the hallmark of the disorder, leads to dysregulated intestinal iron absorption and progressive iron deposition in the liver, heart, skin, endocrine glands, and joints. Survival is normal if organ damage is prevented by early institution of phlebotomy therapy. HH arthropathy is the symptom most affecting quality of life and can be debilitating. Genotype screening in large population studies has shown that the clinical penetrance of C282Y homozygosity is highly variable and can be very low, with up to 50% of women and 20% of men showing a silent phenotype. Targeted population screening for the HFE C282Y mutation is not recommended at present, but might be reconsidered as a cost-effective approach to management if counseling and care were better organized and standardized. Referral of patients to the blood center for phlebotomy therapy and use of HH donor blood for transfusion standardizes treatment, minimizes treatment costs, and may benefit society as a whole. Physician practices should be amended such that HH subjects are more frequently referred to the blood center for therapy.

  15. Relationship between anti-HCV,HCV RNA and ALT in Volunteer Blood Donors%无偿献血者HCV RNA与抗-HCV及ALT检测结果的相关性

    Institute of Scientific and Technical Information of China (English)

    郭燕; 叶世辉; 蔡斌; 景媛媛; 巩晗实; 杨莹; 黄蕾; 段勇

    2016-01-01

    Objective To investigate the relationship between anti-HCV,HCV RNA and ALT in volunteer blood donors. Methods Fluorescent quantitative PCR was used to detect viral loads and kinetic method was employed to assay ALT levels in 235 anti-HCV positive samples who were tested by ELISA. A six month follow up was performed to monitor the anti-HCV(+)/HCV RNA(–) samples. Results Among 235 anti-HCV positive samples,140(59.57%) were positive for HCV RNA. The HCV RNA positive rate in groups of S/CO ratio 1~5 and 5.01~9.99 were 34.29%and 26.47%, respectively. The HCV RNA positive rate in group of S/CO ratio≥10 was 81.68% and statistically significant differences were found between this and the other two groups(χ2 =45.15,P0.05)。抗-HCV(+)/HCV RNA(+)与抗-HCV(+)/HCV RNA(–)组献血者的年龄分别是38.05±11.35岁和33.81±11.50岁,两组的差异有统计学意义(t=2.79,P<0.05)。95份抗-HCV(+)/HCV RNA(–)标本成功回访23例,其中1份标本回访检测抗-HCV转阴。结论 HCV RNA阳性率与抗-HCV的S/CO值有一定相关性,抗-HCV阳性的无偿献血人群中,ALT异常率与HCV RNA无相关性,感染者年龄与HCV RNA有一定相关性。

  16. Seroprevalence for hepatitis E virus (HEV infection among volunteer blood donors of the Regional Blood Bank of Londrina, State of Paraná , Brazil Soroprevalência da infecção pelo virus da hepatite E (VHE em candidatos a doadores de sangue voluntários do Hemocentro Regional de Londrina, Londrina, Estado do Paraná, Brasil

    Directory of Open Access Journals (Sweden)

    André Luiz Bortoliero

    2006-04-01

    Full Text Available A cross-sectional study was carried out among 996 volunteer blood donors enrolled from May 1999 to December 1999 to determine the seroprevalence of hepatitis E virus (HEV infection among volunteer blood donors of the Regional Blood Bank of Londrina, State of Paraná, Brazil, and to evaluate whether the rate of seroprevalence of IgG anti-HEV antibodies is associated with sociodemographic variables and with seropositivity for hepatitis A virus (HAV infection. All participants answered the questionnaire regarding the sociodemographic characterisitcs. Serum samples were tested for IgG antibodies to HEV (anti-HEV by an enzyme linked immunoassay (ELISA. All serum samples positive for anti-HEV IgG and 237 serum samples negative for anti-HEV were also assayed for IgG anti-HAV antibodies by ELISA. Anti-HEV IgG was confirmed in 23/996 samples, resulting in a seroprevalence of 2.3% for HEV infection, similar to previous results obtained in developed countries. No significant association was found between the presence of anti-HEV IgG antibodies and the sociodemographic variables including gender, age, educational level, rural or urban areas, source of water, and sewer system (p > 0.05. Also, no association with seropositivity for anti-HAV IgG antibodies was observed (p > 0.05. Although this study revealed a low seroprevalence of HEV infection in the population evaluated, the results showed that this virus is circulating among the population from Londrina, South Brazil, and point out the need of further studies to define the clinical and epidemiological importance of HEV infection and to identify additional risk factors involved in the epidemiology and pathogenesis of this infection in this population.Os objetivos do estudo foram determinar a soroprevalência da infecção pelo vírus da hepatite E (VHE em candidatos a doadores de sangue voluntários do Hemocentro Regional de Londrina, Paraná, e avaliar se essa soroprevalência está associada com vari

  17. Prevalence of hepatitis B surface antigen (HBsAg) in blood donors from Bombay.

    Science.gov (United States)

    Satoskar, A; Ray, V

    1992-01-01

    Analysis of serum samples from 3104 blood donors from Bombay screened for hepatitis B surface antigen (HBsAg) by ELISA. HBsAg was detected in 4.7% of the subjects. Relatives showed a significantly higher prevalence of HBsAg than volunteer donors. There was no significant association between HBsAg positivity and a particular blood group.

  18. Bone density in apheresis donors and whole blood donors.

    Science.gov (United States)

    Boot, C L; Luken, J S; van den Burg, P J M; de Kort, W L A M; Koopman, M M W; Vrielink, H; van Schoor, N M; den Heijer, M; Lips, P

    2015-11-01

    Apheresis donation using citrate causes acute decrease in serum calcium and increase in serum parathyroid hormone. Long-term consequences, such as decrease in bone mineral density (BMD), are not known. In this study, we compared the BMD of 20 postmenopausal apheresis donors (mean donation number 115 times in up to 15 years) with that of 20 whole blood donors (for 15 years or more) aged 55-70. BMD in the lumbar spine was not lower in apheresis donors than in blood donors (mean ± SD 1.00 ± 0.18 vs. 0.92 ± 0.12, P = 0.09). In the hip, BMD was not different between the groups.

  19. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... Marrow Donor Program® (NMDP), a federally funded nonprofit organization that maintains an international registry of volunteers willing ... Advertise Developers +YouTube Terms Privacy Policy & Safety Send feedback Test new features Loading... Working... Sign in to ...

  20. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... Marrow Donor Program® (NMDP), a federally funded nonprofit organization that maintains an international registry of volunteers willing ... views 1:47 Mike G's Stem Cell Donation Experience - Duration: 4:17. Mike G 5,064 views ...

  1. Increasing demands on today's blood donors

    OpenAIRE

    McClelland, W. M.

    1985-01-01

    Recently in Northern Ireland there has been a rapid increase in demand for a variety of blood components. To meet this need a large proportion of routine blood donations must be processed at the Transfusion Centre. In addition, several blood components are collected direct from donors by apheresis techniques. Apheresis is currently restricted to the collection of components from highly selected donors, but in future this method is likely to be employed for collection of some routine component...

  2. Increasing demands on today's blood donors

    OpenAIRE

    McClelland, W. M.

    1985-01-01

    Recently in Northern Ireland there has been a rapid increase in demand for a variety of blood components. To meet this need a large proportion of routine blood donations must be processed at the Transfusion Centre. In addition, several blood components are collected direct from donors by apheresis techniques. Apheresis is currently restricted to the collection of components from highly selected donors, but in future this method is likely to be employed for collection of some routine component...

  3. Estratégias para fidelização de doadores de sangue voluntários e habituais Estrategias para obtener fidelidad de donadores de sangre voluntarios y habituales Strategies to increase recruitment of voluntary and habitual blood donors

    Directory of Open Access Journals (Sweden)

    Luana Giacomini

    2010-01-01

    . CONCLUSIONES: es indispensable desarrollar una comunicación para reducir miedos y motivar a las personas así como desarrollar una filosofía de atención apoyada en la humanización de las relaciones para obtener la fidelidad de los donadores voluntarios.OBJECTIVES: To understand the meaning of being a blood donor, to describe fears of blood donors regarding blood donation and organization of blood banks, and identify strategies to increase recruitment of voluntary and habitual blood donors. METHODS: This was a qualitative exploratory study. Participants consisted of 15 voluntary blood donors from a local blood bank. Data were collected through taped interviews from January 2007 to March 2007. RESULTS: Blood donation is a solidarity and life saving act. Motivation to donate blood reflected the donor's personal and human decision. There is a need to inform and educate the population regarding blood donation. Attitude of health care professionals can influence the behaviors of blood donors. CONCLUSIONS: There is a need to develop communication strategies to reduce fear and to motivate donation of blood. In addition, there is a need to develop a philosophy of care based on humanization to increase recruitment of voluntary and habitual blood donors.

  4. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... Queue __count__/__total__ Find out why Close Becoming a Blood Stem Cell Donor NCIcancertopics Loading... Unsubscribe from ... later? Sign in to add this video to a playlist. Sign in Share More Report Need to ...

  5. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... Queue __count__/__total__ Find out why Close Becoming a Blood Stem Cell Donor NCIcancertopics Loading... Unsubscribe from ... later? Sign in to add this video to a playlist. Sign in Share More Report Need to ...

  6. How to Motivate Whole Blood Donors to Become Plasma Donors

    Directory of Open Access Journals (Sweden)

    Gaston Godin

    2014-01-01

    Full Text Available This study tested the efficacy of interventions to recruit new plasma donors among whole blood donors. A sample of 924 donors was randomized to one of three conditions: control; information only by nurse; and information plus self-positive image message by nurse (SPI. Participants in the control condition only received a leaflet describing the plasma donation procedure. In the two experimental conditions the leaflet was explained face-to-face by a nurse. The dependent variables were the proportion of new plasma donors and the number of donations at six months. Overall, 141 (15.3% new plasma donors were recruited at six months. There were higher proportions of new plasma donors in the two experimental conditions compared to the control condition (P<.001; the two experimental conditions did not differ. Also, compared to the control condition, those in the experimental conditions (all Ps<.001 gave plasma more often (information only by nurse:  d=.26; SPI: d=.32; the SPI intervention significantly outperformed (P<.05 the information only by nurse condition. The results suggest that references to feelings of SPI such as feeling good and being proud and that giving plasma is a rewarding personal experience favor a higher frequency of plasma donation.

  7. hiv prevalence and demographic risk factors in blood donors

    African Journals Online (AJOL)

    2002-02-02

    Feb 2, 2002 ... blood donors were pre-test counselled and 90% were post test counselled in 200Ö. Conclusions: HIV ... Counselling and serological methods: All potential donors ..... counselling blood donors adds an important advantage in.

  8. Hemochromatosis Patients as Voluntary Blood Donors

    Directory of Open Access Journals (Sweden)

    Tara E Power

    2004-01-01

    Full Text Available The present study was designed to investigate hemochromatosis patients' suitability as blood donors as well as their perceptions and experience with the current public donation system. Participants were gathered from a list of current hemochromatosis patients (n=120 and members of the Canadian Hemochromatosis Society (n=1000. Of the 1120 surveys mailed out to these groups, 801 surveys were returned completed. The sample respondents had a mean age of 57.44 years (SD=12.73; range 19 to 87 years, and 57% were men. It was found that 20% (160 of the respondents have donated blood since their diagnosis; however, only 12% of the respondents indicated that they use voluntary blood donation as a means of maintaining their iron levels. Forty per cent of the respondents indicated that they had been refused from voluntary donation. Despite the fact that in May 2001 the Canadian Blood Services, in collaboration with the Canadian Hemochromatosis Society, began a promotion campaign to encourage hemochromatosis patients to become voluntary blood donors, the present study found that 15% of the respondents reported having been refused from the voluntary blood donation service due to the diagnosis of hemochromatosis. With respect to quality of life, it was found that individuals who donate blood were generally healthier with respect to physical functioning and bodily pain, however, these findings may indicate that hemochromatosis patients who are healthier are better able to donate at public blood banks, rather than that voluntary blood donation has an effect on the donors' physical functioning over phlebotomy clinic users. These study findings suggest that although there may be other medical factors limiting individuals from donating, hemochromatosis patients are interested in being voluntary blood donors and this potential resource is currently under-used.

  9. Blood donation and blood donor mortality after adjustment for a healthy donor effect

    DEFF Research Database (Denmark)

    Ullum, Henrik; Rostgaard, Klaus; Kamper-Jørgensen, Mads;

    2015-01-01

    and mortality. The magnitude of the association was reduced after adjustment for an estimate of self-selection in the donor population. Our observations indicate that repeated blood donation is not associated with premature death, but cannot be interpreted as conclusive evidence of a beneficial health effect.......BACKGROUND: Studies have repeatedly demonstrated that blood donors experience lower mortality than the general population. While this may suggest a beneficial effect of blood donation, it may also reflect the selection of healthy persons into the donor population. To overcome this bias, we...... investigated the relation between blood donation frequency and mortality within a large cohort of blood donors. In addition, our analyses also took into consideration the effects of presumed health differences linked to donation behavior. STUDY DESIGN AND METHODS: Using the Scandinavian Donation...

  10. BLOOD DONOR HAEMATOLOGY PARAMETERS IN TWO ...

    African Journals Online (AJOL)

    hi-tech

    2005-03-03

    Mar 3, 2005 ... (p<0.001) higher Hb, MCV and MCH values while the red blood cell counts and MCHC values were ... a personal medical history and physical examination. In Kenya ... determined and that a donor must be healthy, between.

  11. [Presence of Australia antigen in blood donors].

    Science.gov (United States)

    Gota, F

    1980-01-01

    The differential diagnosis of type A and B viral hepatitis is discussed and guidelines for the prevention of post-transfusional hospital hepatitis are proposed. Methods for the immunological demonstration of HBs antigen are illustrated, together with the respective positivity percentages in blood donors.

  12. A pool of repeat blood donors can be generated with little expense to the blood center in sub-Saharan Africa.

    Science.gov (United States)

    Allain, Jean-Pierre; Sarkodie, Francis; Boateng, Peter; Asenso, Kwame; Kyeremateng, Ernest; Owusu-Ofori, Shirley

    2008-04-01

    In sub-Saharan Africa, most collected blood originates from accessible and cheaper replacement donors while recruiting and retaining volunteers requires considerable costs not all countries can afford. The Kumasi Teaching Hospital Blood Center and a local FM radio station developed a partnership calling three times a year for donation at the radio station where music, entertainment, and token gifts were available. To assess the program's impact, attendance, deferral, age, sex, identification, and viral test results of donors attending 12 consecutive sessions in 2003 through 2006 were analyzed, and this donor population was compared to other types of donors in Kumasi, Ghana. A total of 3801 donors attended the program and 92 percent of the potential FM donors were eligible to donate compared to 85.5 and 70.3 percent of other volunteer and replacement donors, respectively. Ninety percent of donors were male (median, 25 years) and 4.9 percent were hepatitis B surface antigen-positive compared to 11 and 15 percent in other volunteer and replacement donors. This reflected 63.6 percent spontaneous repeat donations from donors responding to the radio appeal compared to 15 to 30 percent in other volunteer donors. It has been demonstrated that the use of a culturally and socially adapted environment to make the gift of blood a pleasurable and festive experience generated a new pool of blood donors spontaneously repeating donations. This program indicates that retaining Ghanaian blood donors is possible at little extra cost to the blood center and that such an approach may represent a substantial help in the efforts of sub-Saharan Africa to collect volunteer blood.

  13. Socio-demographic characteristics of Danish blood donors

    DEFF Research Database (Denmark)

    Burgdorf, Kristoffer Sølvsten; Simonsen, Jacob; Sundby, Anna

    2017-01-01

    Background: Blood transfusion is an essential component of a modern healthcare system. Because knowledge about blood donor demography may inform the design of strategies for donor recruitment and retention, we used nationwide registers to characterize the entire population of blood donors in Denm...

  14. The evaluation of blood donor deferral causes in Zimbabwe

    NARCIS (Netherlands)

    Mafirakureva, N.; Khoza, S.; Van Hulst, M.; Postma, M.J.; Mvere, D.A.

    2013-01-01

    Background: Donor selection is one of the first steps in blood donation aimed at improving the safety of blood and blood products. It is the only line of protection for donors and against certain infections for which testing is not performed. There is paucity of published data on reasons for donor d

  15. Serum ferritin in plateletpheresis and whole blood donors.

    Science.gov (United States)

    Duggan, Frances; O'Sullivan, Kathleen; Power, Joan P; Healy, Michael; Murphy, William G

    2016-08-01

    We performed a prospective analysis of iron status in plateletpheresis donors, using whole blood donors as a control group, to assess the haematinic effects of regular anti-coagulated extracorporeal circulation and platelet collection. Ferritin levels were measured in samples from 31 regular male plateletpheresis donors and from 14 first time male whole blood donors, immediately before and immediately after donation, and immediately before the next donation. An additional 33 regular male plateletpheresis donors and 17 first time male whole blood donors had serum ferritin levels checked predonation. Male plateletpheresis donors had a statistically significant fall in serum ferritin after donation (P = 0.005)*. In addition, male platelet donors had significantly lower serum ferritin levels than first time male blood donors: ferritin serum ferritin measurement in apheresis donor management. Copyright © 2016 Elsevier Ltd. All rights reserved.

  16. Prevalence of seroreactivity among blood donors in rural population.

    Directory of Open Access Journals (Sweden)

    Sonwane B

    2003-09-01

    Full Text Available The seroreactivity pattern amongst blood donors in rural population was studied at S.R.T. Rural Medical College and Hospital, Ambajogai (M. S.. The study period was from January 1996 to December 2001. A total number of 12,240 blood donors were screened. The voluntary donation was 36.98% and replacement donors were 63.02%. No professional donor is bled in our blood bank. The HIV seroreactivity among voluntary donors was 1.56% and 2.11% in replacement donors. The HBsAg seroreactivity was 2.78% in voluntary donors and 4.84% in replacement donors. VDRL seroreactivity is 1.12% in replacement donors. No malarial parasite and HCV seroreactive donor was found in our study period. We have found the magnitude of hepatitis to be far more than that of HIV. Hence testing for HCV routinely is mandatory, besides HBsAg.

  17. Iron stores in regular blood donors in Lagos, Nigeria

    Directory of Open Access Journals (Sweden)

    Adediran A

    2013-06-01

    Full Text Available Adewumi Adediran,1 Ebele I Uche,2 Titilope A Adeyemo,1 Dapus O Damulak,3 Akinsegun A Akinbami,4 Alani S Akanmu1 1Department of Hematology and Blood Transfusion, University of Lagos, Lagos, Nigeria; 2Department of Hematology and Blood Transfusion, Lagos University Teaching Hospital, Lagos, Nigeria; 3Department of Hematology and Blood Transfusion, Jos University Teaching Hospital, Jos, Nigeria; 4Department of Hematology and Blood Transfusion, Lagos State University, Ikeja, Nigeria Background: Apart from challenging the bone marrow to increase its red cell production, thereby producing more blood for the donor, regular blood donation has been shown to have several benefits, one of which is preventing accumulation of body iron which can cause free radical formation in the body. This study was carried out to assess body iron stores in regular blood donors. Methods: A total of 52 regular (study and 30 first-time (control volunteer blood donors were studied prospectively. Twenty milliliters of venous blood was drawn from each subject, 5 mL of which was put into sodium ethylenediamine tetra-acetic acid specimen bottles for a full blood count, including red blood cell indices. The remaining sample was allowed to clot in a plain container, and the serum was then retrieved for serum ferritin, serum iron, and serum transferrin receptor measurement by enzyme-linked immunosorbent assay. Results: Mean hemoglobin and packed cell volume in the study group (13.47 ± 2.36 g/dL and 42.00 ± 7.10, respectively, P = 0.303 were not significantly higher than in the control group (12.98 ± 1.30 g/dL and 39.76 ± 4.41, respectively, P = 0.119. Mean serum ferritin was 102.46 ± 80.26 ng/mL in the control group and 41.46 ± 40.33 ng/mL in the study group (P = 0.001. Mean serum ferritin for women in the study group (28.02 ± 25.00 ng/mL was significantly lower than for women in the control group (56.35 ± 34.03 ng/mL, P = 0.014. Similarly, men in the study group had a lower

  18. Prediction models for hemoglobin deferral in whole blood donors

    NARCIS (Netherlands)

    Baart, A.M.

    2013-01-01

    Each year, a relevant proportion of the invited blood donors is eventually deferred from donation because of low hemoglobin (Hb) levels. Deferrals are meant to protect donors from developing iron deficiency anemia after a blood donation, however, they may increase the risk of donor lapse, even thoug

  19. Eligibility and Exclusion of Hemochromatosis Patients as Voluntary Blood Donors

    Directory of Open Access Journals (Sweden)

    M Levstik

    1998-01-01

    Full Text Available BACKGROUND: Hereditary hemochromatosis patients are excluded in many countries as voluntary blood donors. In 1991, changes in the Canadian Red Cross policy allowed healthy hemochromatosis patients to become voluntary donors.

  20. Risk Factors and Screening for Trypanosoma cruzi Infection of Dutch Blood Donors.

    Directory of Open Access Journals (Sweden)

    Ed Slot

    Full Text Available Blood donors unaware of Trypanosoma cruzi infection may donate infectious blood. Risk factors and the presence of T. cruzi antibodies in at-risk Dutch blood donors were studied to assess whether specific blood safety measures are warranted in the Netherlands.Birth in a country endemic for Chagas disease (CEC, having a mother born in a CEC, or having resided for at least six continuous months in a CEC were considered risk factors for T. cruzi infection. From March through September 2013, risk factor questions were asked to all donors who volunteered to donate blood or blood components. Serum samples were collected from donors reporting one or more risk factors, and screened for IgG antibodies to T. cruzi by EIA.Risk factors for T. cruzi infection were reported by 1,426 of 227,278 donors (0.6%. Testing 1,333 at-risk donors, none (0.0%; 95%, CI 0.0-0.4% was seroreactive for IgG antibodies to T. cruzi. A total of 472 donors were born in a CEC; 553 donors reported their mother being born in a CEC; and 1,121 donors reported a long-term stay in a CEC. The vast majority of reported risk factors were related to Suriname and Brazil. Overall, the participants resided for 7,694 years in CECs, which equals 2.8 million overnight stays. Of those, 1.9 million nights were spent in Suriname.Asymptomatic T. cruzi infection appears to be extremely rare among Dutch blood donors. Blood safety interventions to mitigate the risk of T. cruzi transmission by transfusion would be highly cost-ineffective in the Netherlands, and are thus not required.

  1. Determine The Factors Affecting The Blood Donors Of Selecting Blood Donor Program Me In Western Province Sri Lanka

    Directory of Open Access Journals (Sweden)

    Perera D. A. K.

    2015-08-01

    Full Text Available Abstract Blood and blood component transfusion is one of the major therapeutic practices throughout the world. National Blood Transfusion Service NBTS in Sri Lanka requires approximately 300000 blood units annually. After initiating mobile donor programme there have been two types of blood donation programs in Sri Lanka since 1980. Since second half of first decade of 21st century Sri Lanka shifted to 100 non-replacement blood transfusion policy. That means whole blood and blood component requirement of NBTS has to be collected through mobile blood donor program and voluntary In-house blood donor program. Therefore the objective of this study was to determine the factors affecting the blood donors of selecting blood donor program in Western province Sri Lanka. Methodology This was a cross sectional descriptive study. The study composed of two components. .First the factors that cause the blood donor to select a blood donor programme second the facility survey of blood banks In-house donation. An interviewer administered questionnaire was used to collect data from a sample of 410 Mobile blood donors. Facility survey was done using a checklist. The dependant variables were the attendance of the blood donors to Mobile blood donation and In-house blood donation. Independent variables included were the factors related to socio demography service quality accessibility availability and intrinsic extrinsic motivation. The analytical statistics applied for testing the association of factors with the blood donor programme was chi-square test. The study has shown some important findings. There was significant association between income level and donating blood. Only 3.3 of In-house blood donor population was female. Majority of In-house population belonged to 30-41 age group. A statistically significant association exists between age and repeat blood donation. The female blood donors tendency of becoming repeat donors was very low. Distance problem and non

  2. Application of CART Algorithm in Blood Donors Classification

    Directory of Open Access Journals (Sweden)

    T. Santhanam

    2010-01-01

    Full Text Available Problem statement: This study used data mining modeling techniques to examine the blood donor classification. The availability of blood in blood banks is a critical and important aspect in a healthcare system. Blood banks (in the developing countries context are typically based on a healthy person voluntarily donating blood and is used for transfusions or made into medications. The ability to identify regular blood donors will enable blood banks and voluntary organizations to plan systematically for organizing blood donation camps in an effective manner. Approach: Identify the blood donation behavior using the classification algorithms of data mining. The analysis had been carried out using a standard blood transfusion dataset and using the CART decision tree algorithm implemented in Weka. Results: Numerical experimental results on the UCI ML blood transfusion data with the enhancements helped to identify donor classification. Conclusion: The CART derived model along with the extended definition for identifying regular voluntary donors provided a good classification accuracy based model.

  3. Whole blood donor deferral analysis at a center in Western India

    Directory of Open Access Journals (Sweden)

    Agnihotri Naveen

    2010-01-01

    Full Text Available Introduction: Deferrals lead to loss of precious whole blood donors (WBD and blood units available for transfusion purposes. Knowledge of rate and causes of donor deferral can guide the recruitment strategy for WBD. Aim: To find the incidence and causes of deferral in Indian WBD and apply relevant findings to modify recruitment strategy for blood donors. Materials and Methods: Data for WBD presenting for donation in a blood center and outdoor camps over one and half year were analyzed retrospectively. National guidelines were used for selection and deferral of WBD. Result: 736 (11.6% WBD were deferred out of 6357 presenting for donation during the study period. Most (69.8% of the donors were deferred on physical examination and hemoglobin (Hb testing. Most common reasons for deferral were low Hb (55.8%, abnormal blood pressure (11.1%, medication (6.9% and underweight donors (2.9%. Significantly more volunteers were deferred than relative donors (13.97% vs 5.80%; P<0.000. Females were found to have higher deferral rate than males (53.5% vs 6.9%; P=0.000 and higher odds ratio for deferral (15.4. Donors older than 40 years of age had significantly higher chance of being deferred (P<0.05. Discussion and Conclusion: It is important to determine the rate and causes of WBD deferral to guide the recruitment and retention efforts at local, regional, and national level.

  4. Thalassemia and Hemoglobin E in Southern Thai Blood Donors

    OpenAIRE

    Manit Nuinoon; Kwanta Kruachan; Warachaya Sengking; Dararat Horpet; Ubol Sungyuan

    2014-01-01

    Thalassemia and hemoglobin E (Hb E) are common in Thailand. Individuals with thalassemia trait usually have a normal hemoglobin concentration or mild anemia. Therefore, thalassemic individuals who have minimum acceptable Hb level may be accepted as blood donors. This study was aimed at determining the frequency of α-thalassemia 1 trait, β-thalassemia trait, and Hb E-related syndromes in Southern Thai blood donors. One hundred and sixteen voluntary blood donors, Southern Thailand origin, were ...

  5. Digital questionnaire platform in the Danish Blood Donor Study

    DEFF Research Database (Denmark)

    Burgdorf, K S; Felsted, N; Mikkelsen, S

    2016-01-01

    OBJECTIVES: The Danish Blood Donor Study (DBDS) is a prospective, population-based study and biobank. Since 2010, 100,000 Danish blood donors have been included in the study. Prior to July 2015 all participating donors had to complete a paper-based questionnaire. Here we describe the establishment...... with the questionnaire data in the DBDS database. RESULTS: The digital platform enables personalized questionnaires, presenting only questions relevant to the specific donor by hiding unneeded follow-up questions on screening question results. New versions of questionnaires are immediately available at all blood...

  6. Questionnaire-Related Deferrals in Regular Blood Donors in Norway

    Directory of Open Access Journals (Sweden)

    Håkon Reikvam

    2012-01-01

    Full Text Available Voluntary donation is a key issue in transfusion medicine. To ensure the safety of blood transfusions, careful donor selection is important. Although new approaches to blood safety have dramatically reduced the risks for infectious contamination of blood components, the quality and the availability of blood components depend on the willingness to donate and the reliability of the information given by the donors about their own health, including risk behavior. As donors who are deferred by the blood bank will be less motivated to return for donation, it is important to reduce the number of deferrals. The aims of the present study were to investigate the reasons for deferral of registered donors coming to the blood bank for donation, in order to identify areas of importance for donor education—as these deferrals potentially could be avoided by better donor comprehension. Deferral related to testing of donors is not included in this study as these deferrals are dependent on laboratory results and cannot be indentified by questionnaire or interview. Data were collected from all blood donors in a period for 18 months who came for blood donation at a large university hospital in Norway. 1 163 of the 29 787 regular donors, who showed up for donation, were deferred (3.9%. The main reasons were intercurrent illness (n=182 (15.6%, skin ulcers (n=170 (14.6%, and risk behaviour (n=127 (10.9%. In a community, intercurrent illnesses, skin ulcers, and potential risk behavior are the most frequent reasons for deferral of regular donors. Strategized effort on donor education is needed, as “failure to donate” reduces donor motivation.

  7. Distribution of blood groups in blood donors in the blood bank of Jagdalpur, Bastar district, Chhattisgarh

    Directory of Open Access Journals (Sweden)

    Sachin A Badge

    2017-01-01

    Full Text Available Aims and Objectives: The incidence of ABO and rhesus (Rh groups varies markedly in different races, ethnic groups, and socioeconomic groups in different parts of the world. The frequencies of ABO and Rh blood groups vary from one population to another and time to time in the same region. The present study was carried out to find the distribution of blood group in rural and tribal populations of Bastar district of Chhattisgarh. Materials and Methods: The present retrospective study was carried out at late Shri Baliram Kashyap Memorial Government Medical College and Maharani Hospital blood bank, Jagdalpur, Bastar district, Chhattisgarh, India, during the 2-year period from January 2014 to December 2015. The blood collections were taken from the voluntary donors at outdoor blood donation camp and in-house blood bank as well as from replacement donors at blood bank. Totally 12,852 donors were considered medically fit and accepted for blood donation during the study period. Results: Out of the total 12,852 donors, most of the donors, i.e., 3996 (31.09% were with blood Group O followed by B (30.44%, A (24.95%, and AB (13.52%. Out of the 12,852 blood donors, majority, i.e., 12,779 (99.43% were male and 73 (0.57% were female. Maximum blood donors, i.e., 12,777 (99.42% were Rh positive while only 75 (0.58% were Rh negative. Conclusion: The knowledge of distribution of ABO and Rh blood groups at local and regional levels is helpful in effective management of blood banks and safe blood transfusion services.

  8. The effect of pneumatic tube system on complete blood count parameters and thrombocyte donation in healthy donors.

    Science.gov (United States)

    Sari, Ismail; Arslan, Aliriza; Ozlu, Can; Hacioglu, Sibel; Dogu, Mehmet Hilmi; Isler, Kamil; Keskin, Ali

    2012-08-01

    This paper is the first report whether or not pneumatic tube system affects the selection of apheresis donors according to the results of complete blood count. According to the apheresis guidelines, hemoglobin level must be ≥12.5g/dL and platelet level ≥150/μL to be a donor. Paired blood samples of 26 healthy volunteers were transported by either hand delivered or a pneumatic tube system to the laboratory. No statistically significant differences were observed in order to mean values of routine complete blood cell count and white cell differential parameters that were send for selection of apheresis donor before the procedure. Therefore, all healthy volunteers decided as a donor according to the laboratory results independent from transport method.

  9. REPORT OF HLA DISTRIBUTION IN 2315 VOLUNTEER DONORS OF CHINESE BONE MARROW BANK FROM NORTHWEST CHINA

    Institute of Scientific and Technical Information of China (English)

    Jin Tianbo; Gao Ya; Zhang Hongbo; Zhao Junhai; Lai Jianghua; Lai Shuping; Li Shengbin

    2005-01-01

    Objective To report the HLA data of volunteer donors of Chinese bank from Northwest China and characterize the distribution of HLA genes in Northwest China. Methods HLA-A, B antigens of 2315 volunteer donors were examined by the method of microlymphocytetoxicity (MLT) test .The antigen frequencies(AF) were assessed by directly counting; and based on that gene frequencies(GF) were calculated. HLA data from other population were collected and distribution characteristics were compared. With the raw data, Hardy-Weinberg equilibrium, statistical parameters of forensic medicine interest for HLA were computed. Results A total of 18 specific antigens were detected in HLA-A and the most frequent antigen was A2 . AF and GF were 0.5136 and 0.3026, respectively. A total of 42 specific antigens were detected in HLA-B and the most frequent antigen was A13. Its AF and GF were 0.1978 and 0.1044, respectively. The heterozygosity(H), polymorphism information content(PIC), discrimination power(DP) and probability of paternity exclusion (PPE) of HLA-A were 0.8215, 0.8212, 0.9356 and 0.7798 accordingly; while H,PIC, DP and PPE of HLA-B were 0.9322, 0.9322, 0.9878 and 0.9528. Conclusion The polymorphism of HLA-A,B genes is characteristic in Chinese. In this research, the genetic trait of HLA in 2315 volunteers is consistent with Northern Han population.

  10. The knowledge, attitude and practice towards blood donation among voluntary blood donors in chennai, India.

    Science.gov (United States)

    S, Uma; R, Arun; P, Arumugam

    2013-06-01

    An integrated strategy for blood safety is required for the provision of safe and adequate blood. Recruiting a sufficient number of safe blood donors is an emerging challenge. The shortage of blood in India is due to an increase in the demand, with fewer voluntary blood donors. A study on the knowledge, attitude and the practice of donors may prove to be useful in the successful implementation of the blood donation programme. Our aim was to find the level of the knowledge, attitude and practice of blood donation among voluntary blood donors. A structured questionnaire was given to 530 voluntary blood donors to assess their knowledge, attitude and practice with respect to blood donations. The statistical analyses were done by using the SPSS software. The associations between the demographic factors were analysed by using the Chi square test. Among the 530 donors, 436 (93%) were males and 36 (7%) were female donors. 273 (51.2%) donors knew about the interval of the donation and 421 (79.4%) donors knew about the age limit for the donation. 305 (57%) donors felt that creating an opportunity for the donation was an important factor for motivating the blood donation and 292 (55%) donors felt that the fear of pain was the main reason for the hesitation of the donors in coming forward to donate blood. A majority of the donors were willing to be regular donors. The donors showed positive effects like a sense of satisfaction after the donation. Creating an opportunity for blood donation by conducting many blood donation camps may increase the voluntary blood donations.

  11. Bartonella spp. bacteremia in blood donors from Campinas, Brazil.

    Directory of Open Access Journals (Sweden)

    Luiza Helena Urso Pitassi

    2015-01-01

    Full Text Available Bartonella species are blood-borne, re-emerging organisms, capable of causing prolonged infection with diverse disease manifestations, from asymptomatic bacteremia to chronic debilitating disease and death. This pathogen can survive for over a month in stored blood. However, its prevalence among blood donors is unknown, and screening of blood supplies for this pathogen is not routinely performed. We investigated Bartonella spp. prevalence in 500 blood donors from Campinas, Brazil, based on a cross-sectional design. Blood samples were inoculated into an enrichment liquid growth medium and sub-inoculated onto blood agar. Liquid culture samples and Gram-negative isolates were tested using a genus specific ITS PCR with amplicons sequenced for species identification. Bartonella henselae and Bartonella quintana antibodies were assayed by indirect immunofluorescence. B. henselae was isolated from six donors (1.2%. Sixteen donors (3.2% were Bartonella-PCR positive after culture in liquid or on solid media, with 15 donors infected with B. henselae and one donor infected with Bartonella clarridgeiae. Antibodies against B. henselae or B. quintana were found in 16% and 32% of 500 blood donors, respectively. Serology was not associated with infection, with only three of 16 Bartonella-infected subjects seropositive for B. henselae or B. quintana. Bartonella DNA was present in the bloodstream of approximately one out of 30 donors from a major blood bank in South America. Negative serology does not rule out Bartonella spp. infection in healthy subjects. Using a combination of liquid and solid cultures, PCR, and DNA sequencing, this study documents for the first time that Bartonella spp. bacteremia occurs in asymptomatic blood donors. Our findings support further evaluation of Bartonella spp. transmission which can occur through blood transfusions.

  12. Enhancing blood donation intentions using multimedia donor education materials.

    Science.gov (United States)

    France, Christopher R; France, Janis L; Wissel, Mary Ellen; Kowalsky, Jennifer M; Bolinger, Elizabeth M; Huckins, Jamie L

    2011-08-01

    Prior research has shown that education materials that directly address prospective donor concerns and provide specific coping suggestions are particularly effective at enhancing donation attitudes and intentions to give blood. This study compared the effect of donor coping materials, provided in written and audiovisual formats, as potential tools to enhance recruitment of prospective blood donors. The role of initial attitudes toward blood donation on responses to these materials was also considered. Young adults (62% female; mean [SD] age=19.1 [1.4]; mean [range] prior blood donations=1.32 [0-13]) were randomly assigned to 1) read a brochure addressing common blood donor concerns and suggesting specific coping strategies, 2) view a video addressing blood donor concerns and illustrating coping techniques, 3) read the brochure and view the video, or 4) read a control brochure on healthy eating and exercise. Measures of blood donation attitudes, anxiety, confidence, and intentions to give blood were completed before and after the intervention. Relative to the control brochure, all the intervention groups showed larger reductions in anxiety, more positive changes in attitude, and greater increases in donation confidence and intentions. The combination of the brochure and video outperformed either intervention alone in further improving donation attitudes among participants with high initial donation attitudes. Blood donation coping materials, presented in either written or audiovisual formats, significantly enhance willingness to donate blood among young adults regardless of their initial attitudes toward blood donation. © 2011 American Association of Blood Banks.

  13. Whole blood donor deferral analysis at Genera l hospital blood bank – A retrospective study.

    Directory of Open Access Journals (Sweden)

    Hinal Gajjar

    2014-07-01

    Full Text Available Background: A blood transfusion is a life saving procedure in many instances and it requir es an adequate supply of safe blood. The most important is the selection of blood donors by donor selection criteria. A large number of blood donors are deferred from donating blood for several reasons either temporarily or permanently. A shortage of safe blood donor is frequent and it is important to understand the causes of deferral of potential donors. Aims: 1. Primary objective of this study was to record and document the current rate and reasons for donor deferred in our blood bank. 2. Apply relevant findings to modify recruitment strategy for blood donors. Materials and methods: Data for WBD presenting for blood donation in a blood bank V S General Hospital and outdoor camps over two and half years from January 2011 to June 2013 were analysed retrospectively. National Guidelines were used for selection & deferral of WBD. Results: 3836 (11.16% WBD were deferred out of 34373 registered donors during the study period. Donor deferrals were tabulated into temporary and permanent reasons. Temporary deferrals were 3113 (81.15% and permanent deferrals were 723 (18.85%. The most common reasons were low haemoglobin (48.33%, high blood pressure (11.94%, underweight donors (7.95% and history of medication use (6.13% among total deferral donors. Majority of donors (47.16% deferred were between 18 -30 years. Females were found to have higher deferral rate (25.48% than males (10.76%. Discussion and conclusion: A deferral study in blood donors may shed light on the health status of general population which may affect the present and future blood supply. It is important to provide donors with a clear message on their deferral status so that most of temporary deferral donors will return for donation in future.

  14. Android Blood Donor Life Saving Application in Cloud Computing

    Directory of Open Access Journals (Sweden)

    T.Hilda Jenipha

    2016-07-01

    Full Text Available Emergency situations, such as accidents, create an immediate, critical need for specific blood type. In addition to emergency requirements, advances in medicine have increased the need for blood in many on-going treatments and elective surgeries. Despite increasing requirements for blood, only about 5% of the Indian population donates blood. In this paper we propose a new and efficient way to overcome such scenarios with our project. We have to create a new idea, just touch the button. Donor will be prompted to enter an individual's details, like name, phone number, and blood type. After that your contact details will appear in alphabetical order on the screen; the urgent time of a blood requirement, you can quickly check for contacts matching a particular or related blood group and reach out to them via Phone Call/SMS through the Blood donor App. Blood Donor App provides list of donors in your city/area. Use this app in case of emergency. A large number of blood donors are attracted using an Android application. Cloud- based services can prove important in emergency blood delivery since they can enable central and immediate access to donors' data and location from anywhere. Since almost everyone carries a mobile phone with him, it ensures instant location tracking and communication. The location-based app, operational on android platform, will help users easily find donors of matching blood groups in their location and access their mobile numbers for instant help. Only a registered person, with willingness to donate blood, will be able to access the service.

  15. Applicability of available methods for incidence estimation among blood donors

    Institute of Scientific and Technical Information of China (English)

    Shtmian Zou; Edward P.Notari IV; Roger Y.Dodd

    2010-01-01

    @@ Abstract Incidence rates of major transfusion transmissible viral infections have been estimated threugh widely used sereconversion approaches and recently developed methods.A quality database for blood donors and donations with the capacity to track donation history of each donor is the basis for incidence estimation and many other epidemiological studies.Depending on available data,difierent ways have been used to determine incidence rates based on conversion from uninfected to infected status among repeat donors.

  16. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... been rented. This feature is not available right now. Please try again later. Published on Jul 19, ... her German stem cell donor for the first time in Germany. #priceless - Duration: 1:04. Jacque Brohawn ...

  17. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... be donors at http://www.marrow.org . Category Science & Technology License Standard YouTube License ... - Duration: 49:19. Children's Health 33,509 views 49:19 Stem Cell Fraud: ...

  18. Toxoplasma gondii Seroprevalence Among Blood Donors in Zahedan, Southeastern Iran

    Directory of Open Access Journals (Sweden)

    Jafari Modrek

    2014-07-01

    Full Text Available Background Blood transfusion is one of the many ways, through which Toxoplasma Gondii (T. gondii, a protozoan parasite, can be transmitted to humans. Objectives This cross sectional study aimed to evaluate the seroprevalence of toxoplasma infection and related epidemiological features among healthy blood donors. Materials and Methods A total of 375 blood samples from donors were tested for specific T. gondii antibodies (IgG and IgM by ELISA method, in Blood Transfusion Organization, Zahedan, Iran. Positive samples for T. gondii IgG were further tested for T. gondii IgM. A positive IgG test with a negative or positive IgM test was interpreted as a chronic or acute toxoplasmosis case, respectively. Results From the total 375 blood donors, 94 samples (25% were T. gondii IgG positive. No positive cases of anti-T. gondii IgM antibodies were found. The difference between age and presence of anti-Toxoplasma antibodies was statistically significant (P < 0.05. Other characteristics of blood donors, including male gender, ABO blood groups and having a history of blood transfusion showed no association with infection. Conclusions Our results highlighted that 25% of blood donors were infected by Toxoplasma, prior to the sampling and it can relapse, when facing a decreased immunity level.

  19. Donating blood for research: a potential method for enhancing customer satisfaction of permanently deferred blood donors.

    Science.gov (United States)

    Waller, Daniel; Thijsen, Amanda; Garradd, Allira; Hayman, Jane; Smith, Geoff

    2015-11-30

    Each year, a large number of individuals in Australia are deferred from donating blood. A deferral may have a negative impact on donor satisfaction and subsequent word-of-mouth communication. The Australian Red Cross Blood Service (the Blood Service) is, therefore, investigating options for managing service interactions with deferred donors to maintain positive relationships. While public research institutes in Australia have established independent research donor registries, other countries provide programmes allowing deferred donors to donate blood for research via blood collection agencies. This study examined attitudes towards donating blood for research use in a sample of permanently deferred Australian donors. Donors permanently deferred because of a risk of variant Creutzfeldt-Jakob disease (n=449) completed a postal survey that examined attitudes towards research donation. The majority of participants were interested in donating blood for research (96%), and joining a registry of research donors (93%). Participants preferred to donate for transfusion or clinical research, and were willing to travel large distances. Results indicated that positive attitudes towards the Blood Service would be extended if the opportunity to donate blood was provided. These findings indicate a desire for continued engagement with the Blood Service despite deferral. Donating blood for research is a potential way of maintaining positive relationships with permanently deferred donors which also benefits the health research community. Through maintaining positive relationships with deferred donors, positive word-of-mouth activity can be stimulated. Further work is needed to determine the feasibility of implementing research donation through the Blood Service in Australia.

  20. Socio-demographic characteristics of Danish blood donors

    Science.gov (United States)

    Burgdorf, Kristoffer Sølvsten; Simonsen, Jacob; Sundby, Anna; Rostgaard, Klaus; Pedersen, Ole Birger; Sørensen, Erik; Nielsen, Kaspar René; Bruun, Mie Topholm; Frisch, Morten; Edgren, Gustaf; Erikstrup, Christian; Hjalgrim, Henrik; Ullum, Henrik

    2017-01-01

    Background Blood transfusion is an essential component of a modern healthcare system. Because knowledge about blood donor demography may inform the design of strategies for donor recruitment and retention, we used nationwide registers to characterize the entire population of blood donors in Denmark in 2010. Methods The study population comprised all Danes in the age range eligible for blood donation (N = 3,236,753) at the end of 2010. From the Scandinavian Donations and Transfusions (SCANDAT) register, we identified 174,523 persons who donated blood in Danish blood banks at least once in 2010. The association between sociodemographic characteristics and blood donor prevalence was examined using regression models. Results The overall prevalence of blood donation was 5.4% among both women and men. The age-specific prevalence of blood donation peaked at 25 years of age (6.8%) for women and 30 years of age (5.7%) for men. Children of any age were associated with lower prevalence of blood donation among women, while the opposite was seen for men. Middle to high income groups, but not the highest income group, had fourfold higher donor prevalence than the lowest income group (6.7% compared to 1.7%). The prevalence of blood donation was considerably lower among men living with their parents (2.9%) or alone (3.9%) than among men cohabitating with a woman (6.2%). Summary Social marginalization, as indicated by low income and being a male living without a woman, was associated with lower prevalence of blood donation. However, individuals with very high incomes and women with children were underrepresented in the Danish blood donor population. PMID:28182624

  1. A retrospective cohort study of blood hemoglobin levels in blood donors and competitive rowers

    DEFF Research Database (Denmark)

    Johansson, P. I.; Ullum, H.; Jensen, Kurt;

    2009-01-01

    To investigate the distribution of blood hemoglobin levels in healthy blood donors and elite athletes, a retrospective cohort study from 2001 to 2005 of candidate blood donors and elite rowers in Denmark was performed. Eighty-five thousand eight hundred and forty-six blood donors were identified.......3% of the females demonstrated values above the recommended limit for athletic competition. Thus, the prevalence of a high hemoglobin value was greater in the rowers, of both gender, than in the candidate blood donors (P... people and especially in competition athletes....

  2. The effect of World Blood Donor Day on digital information seeking and donor recruitment.

    Science.gov (United States)

    Kranenburg, Floris J; Kreuger, Aukje L; Arbous, M Sesmu; Laeijendecker, Daphne; van Kraaij, Marian G J

    2017-10-01

    The purpose of World Blood Donor Day (WBDD) is to raise awareness for the importance of blood donation. The aim of this study was to quantify the impact of WBDD on digital information seeking and donor recruitment. Google Trends data were used to quantify seeking behavior on "blood donation" and "blood donor." Differences in relative search volume (RSV) between the 3 weeks surrounding WBDD and the rest of the year were calculated. Second, mean differences in RSV were compared to assess the additional effect of hosting using translated search terms. Third, we compared the period around WBDD with the control period regarding page views of the Sanquin website and Facebook likes and number of newly registered donors in 2016. The mean RSV for "blood donation" in the period of interest was 78.6, compared to 72.1 in the control period (difference, 6.5; 95% confidence interval [95% CI], 1.2-11.8). For "blood donor" this was 78.9 compared to 65.9 (difference, 12.9; 95% CI, 8.1-17.8). We found no additional effect of hosting. In the period of interest, the website of Sanquin was visited 6862 times a day and 4293 times in the control period (difference, 2569; 95% CI, 1687-3451). In June 2016, 54.6% (95% CI, 53.0-56.2) more new donors were registered compared to the control period. An international campaign like WBDD raises the awareness of blood donation and is effective in convincing people to register as blood donors. © 2017 AABB.

  3. Volunteering

    OpenAIRE

    Hustinx, Lesley; Handy, Femida; Cnaan, Ram A

    2010-01-01

    In recent decades, there has been a burgeoning interest in the study of volunteering, and the number of publications devoted to volunteering has grown exponentially. In this chapter, we examine emerging theories and new directions in volunteering research. First, we discuss multi-level perspectives that try to understand volunteering in complex interaction with the organizational and institutional context. Next, we present process-oriented approaches that focus on the experience of volunteeri...

  4. Blood donor motivation: a survey of minority college students.

    Science.gov (United States)

    Oswalt, R; Gordon, J

    1993-06-01

    Ninety-five percent of all blood donors are Caucasian. To investigate why minorities are under-represented as blood donors, a random sample of 120 minority college students were surveyed about attitudes and behavior regarding blood donations. This sample of minority students had donated blood at the same rate (33%) and for the same positive motivation (altruism) and negative motivations (for those who did not donate--fear, medical excuses, didn't think of it, no time) as Caucasian donors. The low rate of blood donations by minorities is not due to their membership in ethnic groups per se, but to other variables such as education and socioeconomic level. A recruitment strategy based on the results of this study is presented.

  5. Evaluation of VDRL test in Sudanese blood donors.

    Science.gov (United States)

    Omer, E F; El Sheikh, F S; Khalil, I A

    1982-04-01

    2201 blood donors and 199 patients with sexually transmitted diseases (STD) were tested with the Venereal Disease Research Laboratory (VDRL) and the fluorescent treponemal antibody-absorption (FTA-ABS) tests. The VDRL test was found positive in 30 (1.36%) of the blood donors and 11 (5.5%) of the patients. Syphilis was confirmed by FTA-ABS test in one patient (0.5%) but not in blood donors. The biological false positive (BFP) reactions were 1.36% in blood donors and 5.0% in control patients. The place of VDRL test as a screening test for syphilis and the low rate of its seroreactivity among blood donors was discussed. The BFP reactions were reviewed to assist in the interpretation of the VDRL test which was evaluated on the basis of the control specimens. It was concluded that syphilis should be excluded before blood is transfused and the VDRL test should remain the screening test of choice in the blood transfusion service.

  6. Effect of laser irradiation of donor blood on erythrocyte shape.

    Science.gov (United States)

    Baibekov, I M; Ibragimov, A F; Baibekov, A I

    2012-04-01

    Changes in erythrocyte shape in donor blood during storage and after irradiation with He-Ne laser and infrared laser were studied by scanning electron microscopy, thick drop express-method, and morphometry. It was found that laser irradiation delayed the appearance of erythrocytes of pathological shapes (echinocytes, stomatocytes, etc.) in the blood; He-Ne laser produced a more pronounced effect.

  7. Partial phenotyping in voluntary blood donors of Gujarat State

    Directory of Open Access Journals (Sweden)

    Maitrey Gajjar

    2016-01-01

    Full Text Available Introduction: Partial phenotyping of voluntary blood donors has vital role in transfusion practice, population genetic study and in resolving legal issues.The Rh blood group is one of the most complex and highly immunogenic blood group known in humans. The Kell system, discovered in 1946, is the third most potent system at triggering hemolytic transfusion reactions and consists of 25 highly immunogenic antigens. Knowledge of Rh & Kell phenotypes in given population is relevant for better planning and management of blood bank; the main goal is to find compatible blood for patients needing multiple blood transfusions. The aim of this study was to evaluate the frequency of Rh & Kell phenotype of voluntary donors in Gujarat state. Materials and Methods: The present study was conducted by taking 5670 samples from random voluntary blood donors coming in blood donation camp. Written consent was taken for donor phenotyping. The antigen typing of donors was performed by Qwalys-3(manufacturer: Diagast by using electromagnetic technology on Duolys plates. Results: Out of 5670 donors, the most common Rh antigen observed in the study population was e (99.07% followed by D (95.40%, C (88.77%, c (55.89% and E (17.88%. The frequency of the Kell antigen (K was 1.78 %. Discussion: The antigen frequencies among blood donors from Gujarat were compared with those published for other Indian populations. The frequency of D antigen in our study (95.4% and north Indian donors (93.6 was significantly higher than in the Caucasians (85% and lower than in the Chinese (99%. The frequencies of C, c and E antigens were dissimilar to other ethnic groups while the ′e′ antigen was present in high frequency in our study as also in the other ethnic groups. Kell antigen (K was found in only 101 (1.78 % donors out of 5670. Frequency of Kell antigen in Caucasian and Black populations is 9% & 2% respectively. The most common Kell phenotype was K-k+, not just in Indians (96.5% but

  8. Partial phenotyping in voluntary blood donors of Gujarat State.

    Science.gov (United States)

    Gajjar, Maitrey; Patel, Tarak; Bhatnagar, Nidhi; Patel, Kruti; Shah, Mamta; Prajapati, Amit

    2016-01-01

    Partial phenotyping of voluntary blood donors has vital role in transfusion practice, population genetic study and in resolving legal issues. The Rh blood group is one of the most complex and highly immunogenic blood group known in humans. The Kell system, discovered in 1946, is the third most potent system at triggering hemolytic transfusion reactions and consists of 25 highly immunogenic antigens. Knowledge of Rh & Kell phenotypes in given population is relevant for better planning and management of blood bank; the main goal is to find compatible blood for patients needing multiple blood transfusions. The aim of this study was to evaluate the frequency of Rh & Kell phenotype of voluntary donors in Gujarat state. The present study was conducted by taking 5670 samples from random voluntary blood donors coming in blood donation camp. Written consent was taken for donor phenotyping. The antigen typing of donors was performed by Qwalys-3(manufacturer: Diagast) by using electromagnetic technology on Duolys plates. Out of 5670 donors, the most common Rh antigen observed in the study population was e (99.07%) followed by D (95.40%), C (88.77%), c (55.89%) and E (17.88%). The frequency of the Kell antigen (K) was 1.78 %. The antigen frequencies among blood donors from Gujarat were compared with those published for other Indian populations. The frequency of D antigen in our study (95.4%) and north Indian donors (93.6) was significantly higher than in the Caucasians (85%) and lower than in the Chinese (99%). The frequencies of C, c and E antigens were dissimilar to other ethnic groups while the 'e' antigen was present in high frequency in our study as also in the other ethnic groups. Kell antigen (K) was found in only 101 (1.78 %) donors out of 5670. Frequency of Kell antigen in Caucasian and Black populations is 9% & 2% respectively. The most common Kell phenotype was K-k+, not just in Indians (96.5%) but also in Caucasians (91%), Blacks (98%) and Chinese (100%). Phenotype

  9. Predictors of hemoglobin in Danish blood donors

    DEFF Research Database (Denmark)

    Kotzé, Sebastian R; Pedersen, Ole B; Petersen, Mikkel S

    2015-01-01

    is a population-based study and biobank. We performed multivariable linear regression analysis to assess the effects of donation activity, physiologic and lifestyle factors, and diet on Hb levels among 15,197 donors. We also performed multivariable logistic regression to evaluate the effects of these factors...... on the risk of having low Hb (defined as Hb below the 10th percentile among men and women, respectively) and of a decrease in Hb greater than 0.5 mmol/L (0.8 g/dL) between successive donations. All analyses were performed stratified for sex and smoking status. We also tested a previously used model...

  10. Relationship between Serum Iron Profile and Blood Groups among the Voluntary Blood Donors of Bangladesh.

    Science.gov (United States)

    Hoque, M M; Adnan, S D; Karim, S; Al-Mamun, M A; Faruki, M A; Islam, K; Nandy, S

    2016-04-01

    Blood donation results in a substantial iron loss and subsequent mobilization from body stores. Chronic iron deficiency is a well-recognized complication of regular blood donation. The present study conducted to compare the level of serum ferritin, serum iron, total iron binding capacity (TIBC) and percentage transferrin saturation in different ABO and Rhesus type blood groups among the voluntary blood donors of Bangladesh. The present prospective study included 100 healthy voluntary donors attending at Department of Blood Transfusion, Dhaka Medical College, Dhaka between the periods of July 2013 to Jun 2014. From each donor 10mL venous blood sample was taken and divided into heparinized and non-heparinized tubes for determination of hemoglobin (Hb), hematocrit (Hct), serum iron (SI), total iron binding capacity (TIBC) and serum ferritin by standard laboratory methods. Percentage of transferrin saturation (TS) calculated from serum iron and TIBC. Data were analyzed with SPSS (version 16) software and comparisons between groups were made using student's t-test and one way ANOVA. In the present study mean±SD of age of the respondents was 27.2±6.5 years with a range of 18 to 49 years and 81.0% were male and 19.0% were female. Among the donors 18.0% had blood group A, 35.0% had blood group B, 14.0% had blood group AB and 33.0% had blood group O. Among the donors 91.0% had rhesus positive and 9.0% had rhesus negative. Donors with blood group O had lowest haemoglobin, serum iron and transferring saturation levels. Donors with blood group A had highest TIBC level. Donors with blood group B had lowest serum ferritin level. An independent samples 't' test showed statistically significant difference in serum ferritin and percentage transferrin saturation between blood group AB and blood group O and in percentage transferrin saturation between blood group B and blood group O. One way ANOVA showed that there is no significant difference in haemoglobin, serum iron, serum

  11. [Marrow donor registration and cord blood banking: current issues].

    Science.gov (United States)

    Takanashi, Minoko

    2016-03-01

    Marrow donor registration and cord blood banking are essential components of the infrastructure required for unrelated haemopoietic stem cell transplantations. We now have a new law to support and regulate the Marrow Donor Coordination Agency, Cord Blood Banks and the Haematopoietic Stem Cell Provision Support Organization. We also need to have a specific goal for bone marrow and peripheral blood stem cell donor registration, a minimum cord blood bank size, and the demographic data to back the medical needs for unrelated haemopoietic stem cell transplantations. To improve bone marrow and peripheral blood stem cell transplantations, we need to recruit younger adults for marrow registration and make greater efforts to shorten the coordinating period. For cord blood transplantations, uniting and empowering the cord blood collection sites is needed, to encourage and motivate obstetricians and other staff, as the quality of cord blood units is primarily determined during collection. Also, the cord blood banks must work cooperatively to provide cord blood internationally, which includes coordinating with international agencies and their regulations.

  12. Characterization of blood donors with high haemoglobin concentration

    DEFF Research Database (Denmark)

    Magnussen, K; Hasselbalch, H C; Ullum, H;

    2013-01-01

    Background and Objectives  The literature contains little on the prevalence and causes of high predonation haemoglobin levels among blood donors. This study aimed to characterize and develop an algorithm to manage would-be donors with polycythaemia. Materials and Methods  Between November 2009......, erythropoietin, ferritin, platelet count and leucocyte count, JAK2 V617 and JAK2 exon12 analysis, as well as other routine measurements. Results  Among 46 such donors, 39 had a history of smoking, which contributes to erythrocytosis. Two had PV, five had severe hypertension, one of them because of renal artery...

  13. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... takes you through her blood stem cell donation experience at the National Institutes of Health Clinical Center ... views 11:58 Sara's filgrastim and PBSC donation experience - Duration: 10:58. Be The Match 24,944 ...

  14. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... navigation Sign in Search Loading... Close Yeah, keep it Undo Close This video is unavailable. Watch Queue ... trial of YouTube Red. Working... No thanks Try it free Find out why Close Becoming a Blood ...

  15. Thalassemia and Hemoglobin E in Southern Thai Blood Donors

    Directory of Open Access Journals (Sweden)

    Manit Nuinoon

    2014-01-01

    Full Text Available Thalassemia and hemoglobin E (Hb E are common in Thailand. Individuals with thalassemia trait usually have a normal hemoglobin concentration or mild anemia. Therefore, thalassemic individuals who have minimum acceptable Hb level may be accepted as blood donors. This study was aimed at determining the frequency of α-thalassemia 1 trait, β-thalassemia trait, and Hb E-related syndromes in Southern Thai blood donors. One hundred and sixteen voluntary blood donors, Southern Thailand origin, were recruited for thalassemia and Hb E screening by red blood cell indices/dichlorophenolindophenol precipitation test. β-Thalassemia and Hb E were then identified by high performance liquid chromatography and 4 common α-thalassemia deletions were characterized by a single tube-multiplex gap-polymerase chain reaction. Overall frequency of hemoglobinopathies was 12.9%, classified as follows: homozygous α-thalassemia 2 (1.7%, heterozygous α-thalassemia 1 (1.7%, heterozygous β-thalassemia without α-thalassemia (0.9%, heterozygous Hb E without α-thalassemia (5.2%, double heterozygotes for Hb E/α-thalassemia 1 (1.7%, homozygous Hb E without α-thalassemia (0.9%, and homozygous Hb E with heterozygous α-thalassemia 2 (0.9%. The usefulness of thalassemia screening is not only for receiving highly effective red blood cells in the recipients but also for encouraging the control and prevention program of thalassemia in blood donors.

  16. Gilbert′s syndrome in healthy blood donors what next??

    Directory of Open Access Journals (Sweden)

    Rajendra G Kulkarni

    2016-01-01

    Full Text Available Settings: This study was done in a tertiary care hospital having bed strength of more than 700 beds at SDM Medical College of Medical Sciences and Hospital, Dharwad, located in Northern Karnataka. Aim: The study was done to ascertain prevalence of Gilbert′s syndrome in healthy blood donors and review the literature about feasibility of utilizing blood components from Gilbert′s syndrome donors. Materials and Methods: The study was done for 18 months and 7030 whole blood units were collected and all the units were subjected to mandatory transfusion-transmitted screening and all the plasma bags which were icteric on visual inspection were subjected to hematological and biochemical investigations to rule out other causes of hyperbilirubinemia. Results: Seven thousand and thirty units were collected and 445 (6.3% were discarded due to various reasons. Of them, 50 units (0.71% had Gilbert′s syndrome. All had unconjugated hyperbilirubinemia and other hematological and liver function tests were within normal range. Statistical analysis was done to find mean, median, and standard deviation from mean and standard error of mean with lower and upper confidence limits. Conclusion: Majority of blood donors whose plasma is icteric are suffering from Gilbert′s syndrome (GS. This disease does not cause any harm to donor or patient but raises a lot of concern as many severe disorders also manifest in similar way. The available literature shows that all blood components can be used from donors suffering from GS. There should be introspection. Proper guidelines are to be framed about the use and discarding of blood components in donors with GS.

  17. Proportion of Rh phenotypes in voluntary blood donors.

    Science.gov (United States)

    Sarkar, R S; Philip, Joseph; Mallhi, R S; Yadav, Pramod

    2013-10-01

    The Rh system is the major blood group system besides ABO system. Even after proper blood grouping and cross matching there is a possibility of alloimmunization and antibody production in the recipients against the Rh or minor blood group antigens like Kell, MNSs, Duffy etc. Keeping in view the heavy financial burden of complete phenotyping of blood; the determination of only Rh phenotypes can play a major role in preventing alloimmunization and adverse events in multitransfusion cases. To determine the proportion of Rh phenotypes in voluntary blood donors with a view to generate blood bank data for constitution of panel of blood donors for multipurpose utilities. Identification of Rhesus factors (Rh) was done by the antigen antibody agglutination test by the test tube method on 10,133 healthy voluntary donors. The phenotypic frequencies of Rh blood groups in the studied population were D-92.25%, C-87.55%, E-26.55%, c-51.06% and e-98.42%. Thus 'e' was the most common and E was the least common of all the Rh types. Phenotypically DCCee group was the most common phenotype and dccee was least common type. Determination of Rh phenotypes can play a major role in preventing alloimmunization and avoiding adverse events in multitransfusion cases.

  18. Donating blood for research: a potential method for enhancing customer satisfaction of permanently deferred blood donors

    Science.gov (United States)

    Waller, Daniel; Thijsen, Amanda; Garradd, Allira; Hayman, Jane; Smith, Geoff

    2017-01-01

    Background Each year, a large number of individuals in Australia are deferred from donating blood. A deferral may have a negative impact on donor satisfaction and subsequent word-of-mouth communication. The Australian Red Cross Blood Service (the Blood Service) is, therefore, investigating options for managing service interactions with deferred donors to maintain positive relationships. While public research institutes in Australia have established independent research donor registries, other countries provide programmes allowing deferred donors to donate blood for research via blood collection agencies. This study examined attitudes towards donating blood for research use in a sample of permanently deferred Australian donors. Materials and methods Donors permanently deferred because of a risk of variant Creutzfeldt-Jakob disease (n=449) completed a postal survey that examined attitudes towards research donation. Results The majority of participants were interested in donating blood for research (96%), and joining a registry of research donors (93%). Participants preferred to donate for transfusion or clinical research, and were willing to travel large distances. Results indicated that positive attitudes towards the Blood Service would be extended if the opportunity to donate blood was provided. These findings indicate a desire for continued engagement with the Blood Service despite deferral. Discussion Donating blood for research is a potential way of maintaining positive relationships with permanently deferred donors which also benefits the health research community. Through maintaining positive relationships with deferred donors, positive word-of-mouth activity can be stimulated. Further work is needed to determine the feasibility of implementing research donation through the Blood Service in Australia. PMID:26674813

  19. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... 53 PBSC (Peripheral blood stem cell) Harvest - Duration: 2:55. bmdpsg 4,554 views 2:55 Introduction To Stem Cells – Manipal Hospital - Duration: ... in Germany. #priceless - Duration: 1:04. Jacque Brohawn 2,548 views 1:04 Stem Cell Basics - How ...

  20. Effects of midazolam on cerebral blood flow in human volunteers

    Energy Technology Data Exchange (ETDEWEB)

    Forster, A.; Juge, O.; Morel, D.

    1982-06-01

    The effects of intravenously administered midazolam on cerebral blood flow were evaluated in eight healthy volunteers using the /sup 133/Xe inhalation technique. Six minutes after an intravenous dose of 0.15 mg/kg midazolam, the cerebral blood flow decreased significantly (P less than 0.001) from a value of 40.6 +/- 3.3 to a value of 27.0 +/- 5.0 ml . 100 g-1 . min-1. Cerebrovascular resistance (CVR) increased from 2.8 +/- 0.2 to 3.9 to 0.6 mmHg/(ml . 100 g-1 . min-1)(P less than 0.001). Mean arterial blood pressure decreased significantly (P less than 0.05) from 117 +/- 8 to 109 +/- 9 mmHg and arterial carbon dioxide tension increased from 33.9 +/- 2.3 to 38.6 +/- 3.2 mmHg (P less than 0.05). Arterial oxygen tension remained stable throughout the study, 484 +/- 95 mmHg before the administration of midazolam and 453 +/- 76 mmHg after. All the subjects slept after the injection of the drug and had anterograde amnesia of 24.5 +/- 5 min. The decrease in mean arterial blood pressure was probably not important since it remained in the physiologic range for cerebral blood flow autoregulation. The increase in arterial carbon dioxide tension observed after the midazolam injection may have partially counteracted the effect of this new benzodiazepine on cerebral blood flow. Our data suggest that midazolam might be a safe agent to use for the induction of anethesia in neurosurgical patients with intracranial hypertension.

  1. Prevalence of HBV and HCV among blood donors in Kosovo.

    Science.gov (United States)

    Fejza, Hajrullah; Telaku, Skender

    2009-02-13

    Hepatitis is disease of the liver caused by the infectious and non-infectious agents. The aim of study was to analyze the prevalence of HBV and HCV among voluntary blood donors in Kosovo, during 2000-2003. The data from National Center for Blood Transfusion of Kosovo were collected and analyzed through descriptive and comparative epidemiological method of retrospective study. All samples were tested by ELISA test. Out of 70348 samples of the blood donors, 3145 were positive. From overall positive samples, 2939 were HBV positive, 192 HCV positive while 14 samples were positive for both viruses. The HBV prevalence among the blood donors of Kosovo is 4.2%, which range Kosovo to the second zone according to the CDC classification of the geographical spread of the HBV infection. The HCV prevalence among the blood donors in Kosovo is 0.3%. Compared to the other European countries this level of prevalence is relatively low. Age group 30-39 years old was presented with 34.8% of cases. The higher number was among the workers, 842 or 26.8%. Based on the results we can conclude that Kosovo have the similar prevalence for HBV and HCV infections as other South East European countries.

  2. Ethical Aspects of Blood Donors and the Recipients of Their Blood

    Directory of Open Access Journals (Sweden)

    P. J. M. van den Burg

    2012-01-01

    Full Text Available To date medical care is inextricable based on blood donors and blood products. The continuing increase and intensification of tests and guidelines also results in a change in deferral and abnormal test results. Donors and recipients of their blood are faced with this information and are confronted with a kaleidoscope of thoughts and emotions. The discussion with respect to paid versus nonpaid donation is not new, but other aspects are less often discussed. We describe these other aspects for donors and recipients of their blood and hope to open the ethical discussion; if and to what extent we should have limits?

  3. A retrospective cohort study of blood hemoglobin levels in blood donors and competitive rowers

    DEFF Research Database (Denmark)

    Johansson, P.I.; Ullum, H.; Jensen, K.

    2009-01-01

    (36 962 males), and 3.9% of the males had a blood hemoglobin above 10.5 mM, equalling a hematocrit of 51% and, 1.6% of the females had hemoglobin above 9.7 mM, corresponding to a hematocrit above 47%. One thousand four hundred and six rowers (1116 males) were investigated and 10.4% of the males and 8.......3% of the females demonstrated values above the recommended limit for athletic competition. Thus, the prevalence of a high hemoglobin value was greater in the rowers, of both gender, than in the candidate blood donors (P...To investigate the distribution of blood hemoglobin levels in healthy blood donors and elite athletes, a retrospective cohort study from 2001 to 2005 of candidate blood donors and elite rowers in Denmark was performed. Eighty-five thousand eight hundred and forty-six blood donors were identified...

  4. Molecular genotyping of HCV infection in seropositive blood donor

    Science.gov (United States)

    Zarin, Siti Noraziah Abu; Ibrahim, Nazlina

    2013-11-01

    This study is to investigate the prevalence of hepatitis C virus infection in seropositive blood donor. RNA was extracted from 32 positive samples in National Blood Centre and Melaka Hospital. The core and NS5B sequences were obtained from 23 samples. Genotype 3a is most prevalent in this study followed by genotype 1a. Evidence of mixed-genotypes (3a and 1b) infections was found in 5 subjects.

  5. Henan Surveys Blood Donors to Aid AIDS Prevention

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    Central China's Henan Province, one of the worst AIDS-hit provinces in the country, launched a sweeping survey in September of past blood donors to ascertain tile number of HIV-infected individuals to aid AIDS prevention and control work.

  6. The prevalence of transfusion transmitted virus infection in blood donors

    Institute of Scientific and Technical Information of China (English)

    Cheng Hui Huang; Ru Guang Chen; Yu Sen Zhou; Hai Tao Wang; Chun Ying Xie

    2000-01-01

    @@INTRODUCTION A newly discovered DNA virus,transfusion transmitted virus (TTV), was reported as a cause of post-transfusion hepatitis of unknown etiology in Japan[1]. In order to investigate TTV prevalence in southern China, a study was carried out among blood donors, patients with liver diseases and hemodialysis to determine the epidemiological charateristics.

  7. Donor Infectious Disease Testing Multinational assessment of blood ...

    African Journals Online (AJOL)

    Donor Infectious Disease Testing Multinational assessment of blood-borne virus testing and transfusion safety on the African continent... ... AFRICAN JOURNALS ONLINE (AJOL) · Journals · Advanced Search · USING AJOL · RESOURCES ... for poor quality, to define a consensus strategy based on appropriate assays; and

  8. Titers of ABO antibodies in group O blood donors

    Directory of Open Access Journals (Sweden)

    Natalia Dallaval Galvão de França

    2011-01-01

    Full Text Available BACKGROUND: Plasma components of group O blood donations are rarely submitted to ABO antibody titrations even though it is well known that passively acquired antibodies may destroy the recipient's own red cells and tissue grafts. OBJECTIVE: Thus, group O donations stratified by gender and age were randomly titrated to identify the best source of products for apheresis and exsanguinous transfusion. METHODS: Samples from 603 blood donors were tested by ABO antibody titration using the conventional tube technique at room temperature. ABO antibody levels higher than 64 were considered high. After correction for gender, statistical analyses were performed using the Fisher exact and Kruskal-Wallis tests. RESULTS: Most donors in the blood bank were male (65.7%. ABO antibody titers ranged from 1 to 2048. The estimations of prevalence for the titers were: anti-A,B 128 = 2.16%; Anti-A > 128 = 9.29% and anti-B > 128 = 4.81%. Low mean titers for both anti-A and anti-B antibodies were found in over 50-year-old men (p-value = 0.040. High anti-B antibody levels were found in young women (p-value = 0.002. CONCLUSION: This study confirms that over 50-year-old O group men should be selected as blood donors in non-identical ABO transfusion situations. Also, titration of ABO antibodies in blood banks will increase safety in non-identical ABO transfusions.

  9. Prevalence of Rh, Duffy, Kell, Kidd & MNSs blood group antigens in the Indian blood donor population

    Directory of Open Access Journals (Sweden)

    R N Makroo

    2013-01-01

    Interpretation & conclusions: This study found the prevalence of the typed antigens among Indian blood donors to be statistically different to those in the Caucasian, Black and Chinese populations, but more similar to Caucasians than to the other racial groups.

  10. Declining prevalence of hepatitis E antibodies among Danish blood donors

    DEFF Research Database (Denmark)

    Holm, Dorte K; Moessner, Belinda K; Engle, Ronald E

    2015-01-01

    BACKGROUND: The increasing incidence of reported hepatitis E cases in Europe has focused attention on hepatitis E virus (HEV) and the risk of transfusion-transmitted hepatitis E. The aim of this study was to investigate the prevalence of antibodies to HEV (anti-HEV) among Danish blood donors in 2...... the importance of using the same assay when comparing the anti-HEV prevalence in populations over time....

  11. Removal of cytomegalovirus DNA from donor blood by filtration.

    Science.gov (United States)

    Smith, K L; Cobain, T; Dunstan, R A

    1993-04-01

    Blood from five donors, previously shown to be positive for cytomegalovirus (CMV) DNA following polymerase chain reaction (PCR) amplification, was filtered through commercially available leucocyte filters. Analysis of pre- and post-filtration samples by PCR with ethidium bromide staining has shown that filtration was successful in removing CMV DNA from all samples. This is evidence that leucocyte filtration of red cell concentrates may greatly decrease the risk of CMV disease following transfusion to susceptible patients.

  12. Trypanosoma cruzi screening in Texas blood donors, 2008-2012.

    Science.gov (United States)

    Garcia, M N; Woc-Colburn, L; Rossmann, S N; Townsend, R L; Stramer, S L; Bravo, M; Kamel, H; Beddard, R; Townsend, M; Oldham, R; Bottazzi, M E; Hotez, P J; Murray, K O

    2016-04-01

    Chagas disease is an important emerging disease in Texas that results in cardiomyopathy in about 30% of those infected with the parasite Trypanosoma cruzi. Between the years 2008 and 2012, about 1/6500 blood donors were T. cruzi antibody-confirmed positive. We found older persons and minority populations, particularly Hispanic, at highest risk for screening positive for T. cruzi antibodies during routine blood donation. Zip code analysis determined that T. cruzi is associated with poverty. Chagas disease has a significant disease burden and is a cause of substantial economic losses in Texas.

  13. Motivating Factors and Potential Deterrents to Blood Donation in High School Aged Blood Donors

    Directory of Open Access Journals (Sweden)

    Rachel Finck

    2016-01-01

    Full Text Available Background. To ensure an adequate supply of blood, collection centers must design campaigns that successfully recruit and maintain an active donor pool. Understanding factors that motivate and deter individuals from donating may help centers develop targeted recruitment campaigns. These factors among high school aged blood donors have not yet been fully investigated. Study Design and Methods. A voluntary, anonymous survey was administered to student donors at high school mobile blood drives. The survey instrument asked the students to rate several potential motivating factors in their importance in the decision to donate blood and several potential deterring factors in their future decision whether or not to donate blood again. The survey also asked the students to rate the desirability of several potential incentives. Results. Motivating factors that reflected prosocial, empathetic, and altruistic thoughts and beliefs were rated highly by students. Pain from phlebotomy was most commonly chosen as potential deterrent. Movie tickets and cookies/snacks at the drive were rated as the most attractive incentives. Conclusion. High school aged blood donors are similar to other donor groups in their expressed motives for donating blood. This group may be unique in the factors that deter them from donating and in their preferences for different incentives.

  14. Too Many Blood Donors – Response Bias in the Swiss Health Survey 2012

    Science.gov (United States)

    Volken, Thomas; Bänziger, Andreas; Buser, Andreas; Castelli, Damiano; Fontana, Stefano; Frey, Beat M.; Sarraj, Amira; Sigle, Jörg; Thierbach, Jutta; Weingand, Tina; Mansouri-Taleghani, Behrouz

    2016-01-01

    Background Data on blood donor status obtained from general surveys and health interview surveys have been widely used. However, the integrity of data on self-reported blood donor status from surveys may be threatened by sampling and non-sampling error. Our study aimed to compare self-reported blood donors (including one-time as well as regular donors) from the Swiss Health Survey 2012 (SHS) with register-based blood donors recorded by blood establishments and evaluate the direction and magnitude of bias in the SHS. Methods We compared population-weighted SHS point estimates of the number of blood donors with their corresponding 95% confidence intervals to the respective figures from blood donor registries (birth cohorts 1978-1993) and estimates of donors based on period donor tables derived from blood donor registries (birth cohorts 1920-1993). Results In the birth cohorts 1978-1993, the SHS-predicted number of donors was 1.8 times higher than the respective number of donors based on registry data. Adjusting for foreign and naturalized Swiss nationals that immigrated after their 18th birthday, the SHS overall predicted number of donors was 1.6 times higher. Similarly, SHS estimates for the 1920-1993 birth cohorts were 2.4 and 2.1 times higher as compared to register-based estimates. Generally, the differences between SHS and register-based donors were more pronounced in men than in women. Conclusion Self-reported blood donor status in the SHS is biased. Estimates of blood donors are substantially higher than respective estimates based on blood donor registries. PMID:27994526

  15. Antibody to histo-blood group A antigen neutralizes HIV produced by lymphocytes from blood group A donors but not from blood group B or O donors

    DEFF Research Database (Denmark)

    Arendrup, M; Hansen, J E; Clausen, H;

    1991-01-01

    not inhibit the HTLV-IIIB/lyB or the HTLV-IIIB/lyO isolate. Specificity of the MAb-mediated inhibition was shown using A-antigen (tetrasaccharide). Thus, HIV infection of PBMC from donors with blood type A appears to induce expression of host-cell-encoded carbohydrate blood group A epitope on HIV which can...

  16. Handling low hemoglobin and iron deficiency in a blood donor population

    DEFF Research Database (Denmark)

    Magnussen, Karin; Ladelund, Steen

    2016-01-01

    for donor Hb and iron. An algorithm was created based on Hb and F levels, which drove decisions on outreach by the donor Hb and iron resource team to the donor, including whether to provide iron supplementation or, on rare occasions, a referral to the donor's general practitioner. STUDY DESIGN AND METHODS......BACKGROUND: Iron deficiency and blood donors with low hemoglobin (Hb) concentration are well-known challenges in any blood bank setting. In the Capital Region of Denmark, a new approach was adopted that centralized measurement of Hb, initiated ferritin (F) measurement, and established a center......: The change in Hb for repeat donors was followed during the first 2 years of the intervention strategy, which included measurements of F and offering intermittent iron supplementation to some of the donors. RESULTS: In 2 years, 62,663 blood donors donated 193,288 units of blood and 318 donors gave 754...

  17. Applying self-determination theory to the blood donation context: The blood donor competence, autonomy, and relatedness enhancement (Blood Donor CARE) trial.

    Science.gov (United States)

    France, Christopher R; France, Janis L; Carlson, Bruce W; Frye, Victoria; Duffy, Louisa; Kessler, Debra A; Rebosa, Mark; Shaz, Beth H

    2017-02-01

    The Blood Donor Competency, Autonomy, and Relatedness Enhancement (Blood Donor CARE) project was designed as a practical application of self-determination theory to encourage retention of first-time donors. Self-determination theory proposes that people are more likely to persist with behaviors that are internally-motivated, and that externally-motivated behavior can evolve and become internalized given the appropriate socio-environmental conditions. According to self-determination theory, motivation to engage in blood donation may become increasingly self-determined if the behavior satisfies fundamental human needs for competence (a sense of self-efficacy to achieve specific goals), autonomy (a sense of volitional control over one's behavior), and relatedness (a sense of connection to a larger group). The primary aim of this randomized controlled trial is to examine the effect of competence, autonomy, and/or relatedness interventions on donor retention. Using a full factorial design, first-time donors will be assigned to a control condition or one of seven intervention conditions. Donation competence, autonomy, and relatedness, along with additional constructs associated with return donation, will be assessed before and after the intervention using online surveys, and donation attempts will be tracked for one-year using blood center donor databases. We hypothesize that, compared to the control condition, the interventions will increase the likelihood of a subsequent donation attempt. We will also examine intervention-specific increases in competence, autonomy, and relatedness as potential mediators of enhanced donor retention. By promoting first-time donor competence, autonomy, and relatedness our goal is to enhance internal motivation for giving and in so doing increase the likelihood of future donation.

  18. Recruitment of prospective donors: what do they expect from a homepage of a blood transfusion service?

    Science.gov (United States)

    Moog, R; Fourné, K

    2007-08-01

    In times of shrinking donor population, the recruitment of donors is of utmost importance. Recruitment can be done by personal communication, advertisement/information, classical mass media (newspaper, radio, TV) or new computerized media. The aim of this study was to gain information about the donors' demands of an Internet presentation of a blood transfusion service. Between October and December 2004 inclusive, prospective donors were asked to complete a survey about the impact of Internet information for blood donors. The survey contained questions measuring demographics, education and motivation for blood donation. In addition, the survey included questions that measured Internet access, duration of online time and donors' demands for an Internet presentation of a blood transfusion service. Donors were asked to tick a box with predefined answers. In cases where no options were applied, donors were requested to specify their answers. One hundred and fourteen prospective donors (71 female, 43 male) with a median age of 25 years (range 18-57 years) completed the survey. Most donors (57.9%) were 18-30 years old. Forty-two (36.8%) of the surveyed donors were repeat donors, whereas 72 (63.2%) were first-time donors. The majority of donors were informed about blood donation from relatives or friends (70.7% repeat donors and 67.7% first-time donors). Most of them had Internet access (85.7% repeat donors and 90.3% first-time donors). Exclusive use of private access was more often reported in repeat donors (77.8%), whereas both private and professional access was more frequently used in first-time donors (32.3%). Most donors used the Internet access daily, followed by weekly and monthly use. Multiple answers were given about the importance of desired information about the topic 'blood donation'. Both first-time and repeat donors wanted to be informed about organizational details of blood donation such as opening times, eligibility criteria, donation process and the kind

  19. Improving health profile of blood donors as a consequence of transfusion safety efforts

    DEFF Research Database (Denmark)

    Edgren, Gustaf; Tran, Trung Nam; Hjalgrim, Henrik

    2007-01-01

    BACKGROUND: Transfusion safety rests heavily on the health of blood donors. Although they are perceived as being healthier than average, little is known about their long-term disease patterns and to which extent the blood banks' continuous efforts to optimize donor selection has resulted in impro...... nature, have successfully refined the selection of a particularly healthy subpopulation Udgivelsesdato: 2007-Nov......BACKGROUND: Transfusion safety rests heavily on the health of blood donors. Although they are perceived as being healthier than average, little is known about their long-term disease patterns and to which extent the blood banks' continuous efforts to optimize donor selection has resulted...... or to the selection criteria for blood donation. Blood donors recruited in more recent years exhibited a lower relative mortality than those who started earlier. CONCLUSION: Blood donors enjoy better than average health. Explicit and informal requirements for blood donation in Scandinavia, although mostly of a simple...

  20. Motivational differences between whole blood and plasma donors already exist before their first donation experience.

    Science.gov (United States)

    Veldhuizen, Ingrid; van Dongen, Anne

    2013-08-01

    The demand for plasma products has increased rapidly. It is therefore important to understand donating behavior by plasma donors. This study investigates whether motivational differences between whole blood and plasma donors already exist at the beginning of a donor career. New donors (n = 4861) were invited to fill out a questionnaire before their first donation (response, 61%). The questionnaire assessed variables from the Theory of Planned Behavior (intention, self-efficacy, attitude, and norms), conscientiousness, and donation anxiety. Three years later it was determined who became whole blood or plasma donor. Multivariable linear regression analyses for intention were fitted separately for whole blood and plasma donors. A logistic regression analysis was executed to estimate the effect of intention at the beginning of a donor career on becoming a plasma donor. Plasma donors had a higher intention, self-efficacy, attitude, and conscientiousness and a lower anxiety than whole blood donors. In plasma and whole blood donors, both self-efficacy and cognitive attitude were positively related to intention but with different strength (plasma, β = 0.47 and β = 0.30; whole blood, β = 0.57 and β = 0.17). Having a high level of intention increased the odds of becoming a plasma donor (odds ratio, 1.33; 95% confidence interval, 1.12-1.59). Motivational differences already exist between future whole blood and plasma donors before their first donation. Although a feeling of self-efficacy is necessary for all new donors, more favorable cognitions are important for future plasma donors. Recruitment strategies for plasma donors should focus on attracting the more self-confident donors by highlighting the usefulness of plasma donation. © 2012 American Association of Blood Banks.

  1. Assessing anxiety levels and empathic tendency in blood and platelet donors.

    Science.gov (United States)

    Kılıç, Suar Çakı; Doğan, Erdoğan; Sevimligül, Gülgün; Yücel, Birsen; Bolat, Fatih; Kavakçı, Onder; Sencan, Mehmet

    2013-06-01

    In spite of a constantly-increasing requirement for blood transfusion in the world, blood donation does not exhibit an increase at the same rate. In Turkey with a population of 74 million, only 15 per 10,000 people donate blood regularly and rate of voluntary blood donation is very low compared to developed countries. The aim of this study is to determine empathic level of donors and anxiety levels of blood and platelet donors and also to enable comfort and motivation of donors by taking precautions for reducing their anxieties. This prospective and descriptive study was conducted with 100 voluntary donors (50 blood donors, 50 platelet donors) who admitted to Blood Centre of Cumhuriyet University Hospital between 15 March 2012 and 30 April 2012. Average age of these donors was 27 (19-48)years. The mean scores of donors from Empathic Tendency Scale (ETS), State Anxiety Invertory (SAI) and Trait Anxiety Inventory (TAI) were 70 (49-83), 40 (33-45) and 34 (30-44), respectively. ETS score of those donating blood/platelet for the first time was low, >1 is higher in those who donated previously. SAI and TAI scores of blood donors were higher than those of platelet donors (pempathy. Donation made during the continuous disclosure is an important factor for being a donor.

  2. Hepatitis B escape mutants in Scottish blood donors.

    Science.gov (United States)

    Larralde, Osmany; Dow, Brian; Jarvis, Lisa; Davidson, Fiona; Petrik, Juraj

    2013-06-01

    Hepatitis B virus (HBV) remains as the viral infection with the highest risk of transmission by transfusion. This risk is associated with window period donations, occult HBV infection (OBI) and the emergence of escape mutants, which render blood donations false negative for hepatitis B surface antigen (HBsAg) serological testing. A retrospective study was conducted to gain insights into the molecular epidemiology of HBV escape mutants in Scottish blood donors. The criterion for selection was HBV positivity either by serology or nucleic acid testing (NAT). HBsAg detection was compared across several commercial immunoassays. The full length S gene from plasma samples was PCR amplified, cloned and expressed in HepG2 cells. Eight samples showed HBsAg discordant results, while 5 OBI samples were found. Four escape mutants, containing missense mutations in the S gene, are described here. These mutations impaired HBsAg detection both from HBV infected plasma samples and from recombinant proteins derived from its infected donors. Phylogenetic analysis showed that most of the mutants were clustered in the genotype D and were closely related to strains from Asia and the Middle East. We report here a proline substitution, outside the major hydrophilic region, that impaired HBsAg detection in vivo and in vitro, warning about the risk for the emergence of vaccine escape mutants with mutations outside the major neutralisation site.

  3. Biosimilar G-CSF versus filgrastim and lenograstim in healthy unrelated volunteer hematopoietic stem cell donors.

    Science.gov (United States)

    Farhan, Roiya; Urbanowska, Elżbieta; Zborowska, Hanna; Król, Małgorzata; Król, Maria; Torosian, Tigran; Piotrowska, Iwona; Bogusz, Krzysztof; Skwierawska, Kamila; Wiktor-Jędrzejczak, Wiesław; Snarski, Emilian

    2017-08-11

    The World Marrow Donor Organization recommends original granulocyte-colony stimulating factor (G-CSF) for the mobilization of stem cells in healthy unrelated hematopoietic stem cell donors. We report the comparison of a biosimilar G-CSF (Zarzio) with two original G-CSFs (filgrastim and lenograstim) in mobilization in unrelated donors. We included data of 313 consecutive donors who were mobilized during the period from October 2014 to March 2016 at the Medical University of Warsaw. The primary endpoints of this study were the efficiency of CD34+ cell mobilization to the circulation and results of the first apheresis. The mean daily dose of G-CSF was 9.1 μg/kg for lenograstim, 9.8 μg/kg for biosimilar filgrastim, and 9.3 μg/kg for filgrastim (p G-CSF per kilogram (p = 0.787). Target doses of CD34+ cells were reached with one apheresis in 87% donors mobilized with lenograstim and in 93% donors mobilized with original and biosimilar filgrastim (p = 0.005). The mobilized apheresis outcomes (mean number of CD34+ cells/kg of donor collected during the first apheresis) was similar with lenograstim, biosimilar filgrastim, and filgrastim: 6.2 × 10(6), 7.6 × 10(6), and 7.3 × 10(6), respectively, p = 0.06. There was no mobilization failure in any of the donors. Biosimilar G-CSF is as effective in the mobilization of hematopoietic stem cells in unrelated donors as original G-CSFs. Small and clinically irrelevant differences seen in the study can be attributed to differences in G-CSF dose and collection-related factors. Active safety surveillance concurrent to clinical use and reporting to donor outcome registry (e.g., EBMT donor outcome registry or WMDA SEAR/SPEAR) might help to evaluate the possible short- and long-term complications of biosimilar G-CSF.

  4. Association of ABO and Rh Blood Groups to Blood-Borne Infections among Blood Donors in Tehran-Iran

    Science.gov (United States)

    MOHAMMADALI, Fatemeh; POURFATHOLLAH, Aliakbar

    2014-01-01

    Abstract Background The aim of this study was to investigate the prevalence of hepatitis B, hepatitis C, HIV and syphilis infections in blood donors referred to Tehran Blood Transfusion Center (TBTC), and determine any association between blood groups and blood- borne infections between the years of 2005 and 2011. Methods This was a retrospective study conducted at TBTC. All of the donor serum samples were screened for HBV, HCV, HIV and syphilis by using third generation ELISA kits and RPR test. Initial reactive samples were tested in duplicate. Confirmatory tests were performed on all repeatedly reactive donations. Blood group was determined by forward and reverse blood grouping. The results were subjected to chi square analysis for determination of statistical difference between the values among different categories according to SPSS program. Results Overall, 2031451 donor serum samples were collected in 2005-2011. Totally, 10451 were positive test for HBV, HCV, HIV and syphilis. The overall seroprevalence of HBV, HCV, HIV, and syphilis was 0.39%, 0.11%, 0.005%, and 0.010%, respectively. Hepatitis B and HIV infections were significantly associated with blood group of donors (P 0.05). Conclusion Compared with neighboring countries and the international standards, prevalence of blood-borne infections is relatively low. PMID:25909065

  5. Association of ABO and Rh Blood Groups to Blood-Borne Infections among Blood Donors in Tehran-Iran.

    Directory of Open Access Journals (Sweden)

    Fatemeh Mohammadali

    2014-07-01

    Full Text Available The aim of this study was to investigate the prevalence of hepatitis B, hepatitis C, HIV and syphilis infections in blood donors referred to Tehran Blood Transfusion Center (TBTC, and determine any association between blood groups and blood- borne infections between the years of 2005 and 2011.This was a retrospective study conducted at TBTC. All of the donor serum samples were screened for HBV, HCV, HIV and syphilis by using third generation ELISA kits and RPR test. Initial reactive samples were tested in duplicate. Confirmatory tests were performed on all repeatedly reactive donations. Blood group was determined by forward and reverse blood grouping. The results were subjected to chi square analysis for determination of statistical difference between the values among different categories according to SPSS program.Overall, 2031451 donor serum samples were collected in 2005-2011. Totally, 10451 were positive test for HBV, HCV, HIV and syphilis. The overall seroprevalence of HBV, HCV, HIV, and syphilis was 0.39%, 0.11%, 0.005%, and 0.010%, respectively. Hepatitis B and HIV infections were significantly associated with blood group of donors (P 0.05.Compared with neighboring countries and the international standards, prevalence of blood-borne infections is relatively low.

  6. Determining staffing requirements for blood donor clinics: the Canadian Blood Services experience.

    Science.gov (United States)

    Blake, John T; Shimla, Susan

    2014-03-01

    Canadian Blood Services runs approximately 16,000 donor clinics annually. While there were more than 220 different clinic configurations used in 2011 and 2012, 67% of all clinic configurations followed one of 51 standard models. As part of operational planning for current and future configurations it was necessary for Canadian Blood Services to calculate staffing requirements for standard clinic models. In this article we present a method that incorporates both cost control and impact on donor experience. We calculate staffing requirements to minimize costs, but adjust using queuing theory to ensure donor wait time metrics are met. The method can be applied in a wide variety of situations. Although developed for a particular study, the methods described in this article can be applied in a wide variety of situations. A case study in which the model is used to review existing staffing arrangements at Canadian Blood Services is presented. The staffing model can be used to balance the requirements of minimizing staffing costs with that of ensuring that donors do not suffer unnecessary delays. Moreover, in an example application, savings of 3.4% were identified through the modeling process. © 2013 American Association of Blood Banks.

  7. Antibody to histo-blood group A antigen neutralizes HIV produced by lymphocytes from blood group A donors but not from blood group B or O donors

    DEFF Research Database (Denmark)

    Arendrup, M; Hansen, J E; Clausen, H

    1991-01-01

    Three virus isolates HTLV-IIIB/lyA, HTLV-IIIB/lyB and HTLV-IIIB/lyO, obtained by passaging and propagating the HTLV-IIIB/H9 isolate in three separate cultures of mixed peripheral blood mononuclear cells (PBMC) from donors of blood type A, B or O, respectively, were tested for susceptibility...... not inhibit the HTLV-IIIB/lyB or the HTLV-IIIB/lyO isolate. Specificity of the MAb-mediated inhibition was shown using A-antigen (tetrasaccharide). Thus, HIV infection of PBMC from donors with blood type A appears to induce expression of host-cell-encoded carbohydrate blood group A epitope on HIV which can...

  8. Blood donors' perceptions, motivators and deterrents in Sub-Saharan Africa - a scoping review of evidence

    DEFF Research Database (Denmark)

    Asamoah-Akuoko, Lucy; Hassall, Oliver W; Bates, Imelda

    2017-01-01

    save lives. The main deterrent to blood donation was fear due to lack of knowledge and discouraging spiritual, religious and cultural perceptions of blood donation. The main motivators for blood donation were altruism, donating blood for family and incentives. The findings support the need for targeted......Achieving an adequate blood supply in Sub-Saharan Africa (SSA) through donor mobilization and retention is crucial. Factors that motivate or deter blood donors vary according to beliefs and social norms. Understanding the factors that influence blood donation behaviour in SSA is vital to developing...... effective strategies to address blood donor motivation and retention. This review of 35 studies from 16 SSA countries collates available evidence concerning the perceptions, motivators and deterrents that influence blood donors in SSA. The review revealed a common understanding that blood and blood donation...

  9. Caracterización de donantes voluntarios de sangre por grupo sanguíneo A B O y Rh que asistieron a un banco de sangre de la ciudad de Tunja- Colombia./Characterization of voluntary blood donors for blood group ABO and Rh attending a blood bank in the city

    OpenAIRE

    2012-01-01

    Background: The frequency of ABO blood group and Rh factor is an important factor in relation to the needs of blood components in the population, identifying a routine in blood banks.Materials and methods: A retrospective, cross sectional, descriptive study was performed, period from January to March 2012 with data provided by the Foundation from Colombia Hematological volunteer blood donors who attended a fixed point of blood collection in the city of Tunja - Colombia. The variables analyzed...

  10. Development of chagas cardiac manifestations among Texas blood donors.

    Science.gov (United States)

    Garcia, Melissa N; Murray, Kristy O; Hotez, Peter J; Rossmann, Susan N; Gorchakov, Rodion; Ontiveros, Alejandra; Woc-Colburn, Laila; Bottazzi, Maria Elena; Rhodes, Charles E; Ballantyne, Christie M; Aguilar, David

    2015-01-01

    Chagas disease, infection with the parasite Trypanosoma cruzi, has recently been identified as an important emerging parasitic disease in the United States. To describe the cardiac abnormalities in T. cruzi-positive blood donors in southeastern Texas, a pilot study of donors who had screened positive from 2007 to 2012 was performed. This one-time assessment included (1) a questionnaire to evaluate the source of infection, cardiac symptoms, and health co-morbidities; (2) electrocardiography; (3) echocardiography if electrocardiographic findings were abnormal; and (4) measurement of a high-sensitivity troponin T biomarker. Of those with confirmed infection, 41% (7 of 17) had electrocardiographic abnormalities consistent with Chagas cardiomyopathy. In addition, 36% (6 of 17) were suspected to be locally acquired cases. High-sensitivity troponin T serum levels increased with cardiac severity. In conclusion, cardiologists should consider Chagas disease in their differential diagnoses for patients who may have clinically compatible electrocardiographic changes or nonischemic cardiomyopathy, even if the patients have no histories of residing in Chagas-endemic countries. Copyright © 2015 Elsevier Inc. All rights reserved.

  11. Case Report: Successful Sporozoite Challenge Model in Human Volunteers with Plasmodium vivax Strain Derived from Human Donors

    Science.gov (United States)

    2009-01-01

    CBC, reticulocyte count, G-6-PD determination, Duffy phenotype, ABO and Rh group typing, hemoglobin electrophoresis and erythrocyte sedimentation...was considered as an exclusion criterion. Tests for hemoglobin , white blood cell count, platelet count, and total bilirubin were performed again on...malaria-naïve volunteers were randomly allocated to Groups A–C and exposed to 3 ± 1, 6 ± 1, and 9 ± 1 bites of Anopheles albimanus mosquitoes infected

  12. Infection and blood transfusion: a guide to donor screening.

    Science.gov (United States)

    Reine, Nyssa J

    2004-05-01

    In recent years, blood-component therapy has become more accessible in veterinary practice. As with human medicine, care must be taken to minimize the risk of disease transmission from donor to recipient. Determining the appropriate diseases to screen for is complicated by regional variations in disease incidence, the existence of chronic carrier states for some diseases, the difficulty in screening-test selection, and testing cost. The feline diseases considered include retroviral infections, feline coronaviruses, ehrlichiosis (Ehrlichia canis-like), anaplasmosis (Anaplasma phagocytophilum), neorickettsiosis (Neorickettsia risticii), hemoplasmosis (Mycoplasma hemofelis and M. hemominutum, previously feline hemobartonellosis), and cytauxzoonosis (Cytauxzoon felis). The canine diseases considered in this paper include babesiosis (Babesia canis and B. gibsonii,) ehrlichiosis (E. canis and E. ewingii), anaplasmosis (A. phagocytophilum), neorickettsiosis (N. risticii var. atypicalis), leishmaniasis (Leishmania donovani complex), brucellosis (Brucella canis), hemoplasmosis (M. hemocanis, previously canine hemobartonellosis), and bartonellosis (Bartonella vinsonii).

  13. The risk for hepatitis C infection in blood donors in Cluj County, Romania.

    Science.gov (United States)

    Hâţu, G; Brumboiu, M I; Czernichow, P; Bocşan, I S

    2014-05-01

    Blood products safety is based on different criteria including the selection of blood donors. Blood donors referred to Cluj County (Romania) Blood Transfusion Centre in January-March 2012 completed a self-administered questionnaire and were examined by a physician. Data collected from first-time and repeat donors were compared for possible risk factors for hepatitis C infection. In total, 1100 donors were selected. In first-time donors, most frequent factors were age<26 years, female gender and history of health care procedures. Behavioural risk factors (e.g. drug use, sexual promiscuity) may not be properly filtered out in blood donors, suggesting the necessity of improving the health screening process. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

  14. Algorithm for recall of HIV reactive Indian blood donors by sequential immunoassays enables selective donor referral for counseling

    Directory of Open Access Journals (Sweden)

    Thakral B

    2006-01-01

    Full Text Available Background: HIV/AIDS pandemic brought into focus the importance of safe blood donor pool. Aims: To analyze true seroprevalence of HIV infection in our blood donors and devise an algorithm for donor recall avoiding unnecessary referrals to voluntary counseling and testing centre (VCTC. Materials and Methods: 39,784 blood units were screened for anti-HIV 1/2 using ELISA immunoassay (IA-1. Samples which were repeat reactive on IA-1 were further tested using two different immunoassays (IA-2 and IA-3 and Western blot (WB. Based on results of these sequential IAs and WB, an algorithm for recall of true HIV seroreactive blood donors is suggested for countries like India where nucleic acid testing or p24 antigen assays are not mandatory and given the limited resources may not be feasible. Results: The anti-HIV seroreactivity by repeat IA-1, IA-2, IA-3 and WB were 0.16%, 0.11%, 0.098% and 0.07% respectively. Of the 44 IA-1 reactive samples, 95.2% (20/21 of the seroreactive samples by both IA-2 and IA-3 were also WB positive and 100% (6/6 of the non-reactive samples by these IAs were WB negative. IA signal/cutoff ratio was significantly low in biological false reactive donors. WB indeterminate results were largely due to non-specific reactivity to gag protein (p55. Conclusions: HIV seroreactivity by sequential immunoassays (IA-1, IA-2 and IA-3; comparable to WHO Strategy-III prior to donor recall results in decreased referral to VCTC as compared to single IA (WHO Strategy-I being followed currently in India. Moreover, this strategy will repose donor confidence in our blood transfusion services and strengthen voluntary blood donation program.

  15. Hepatitis B Prevalence and Risk Factors in Blood Donors in Ghazvin, IR.Iran|

    Directory of Open Access Journals (Sweden)

    Babak Yektaparast

    2005-12-01

    Full Text Available Background and Aims: This study was done to find out the common routes of transmission of hepatitis B.Methods: We assessed 39598 volunteer blood donors for hepatitis B and C. Risk factors were obtained from 186 patients and 186 healthy donors. Independent risk factors were determined using logistic regression analysis.Results: Prevalence of HBV was 1.08%. Female sex, education level lower than secondary school, being married, and age more than 35 years old, were risk factors in univariate analysis. Logistic regression showed that only duration of marriage, close contact with an HBV infected person, extramarital sexual contact, history of sexually transmitted diseases and high risk jobs were independent risk factors for prediction of hepatitis B infection. Risk factors which were addressed in this study covered 95.7% of the patients. Conclusions: Ghazvin is one of the low prevalent regions for hepatitis B in Iran. Prevalence of hepatitis B is decreasing in comparison with past decades. Horizontal mode is more important than vertical transmission in this region of Iran.Screening programs, education and vaccination, specifically in high risk groups are essential for prevention of new cases

  16. Effect of autologous blood donation on the central venous pressure, blood loss and blood transfusion during living donor left hepatectomy

    Institute of Scientific and Technical Information of China (English)

    Bruno Jawan; Shih-Hor Wang; Chih-Che Lin; Tsan-Shiun Lin; Yueh-Wei Liu; Chao-Long Chen; Yu-Fan Cheng; Chia-Chi Tseng; Yaw-Sen Chen; Chih-Chi Wang; Tung-Liang Huang; Hock-Liew Eng; Po-Ping Liu; King-Wah Chiu

    2005-01-01

    AIM: Autologous blood donation (ABD) is mainly used to reduce the use of banked blood. In fact, ABD can be regarded as acute blood loss. Would ABD 2-3 d before operation affect the CVP level and subsequently result in less blood loss during liver resection was to be determined.METHODS: Eighty-four patients undergoing living donor left hepatectomy were retrospectively divided as group Ⅰ (GⅠ)and group Ⅱ (GⅡ) according to have donated 250-300 mL blood 2-3 d before living donor hepatectomy or not. The changes of the intraoperative CVP, surgical blood loss,blood products used and the changes of perioperative hemoglobin (Hb) between groups were analyzed and compared by using Mann-Whitney Utest.RESULTS: The results show that the intraoperative CVP changes between GⅠ (n = 35) and GⅡ (n = 49) up to graft procurement were the same, subsequently the blood loss,but ABD resulted in significantly lower perioperative Hb levels in GⅠ.CONCLUSION: Since none of the patients required any blood products perioperatively, all the predonated bloods were discarded after the patients were discharged from the hospital, It indicates that ABD in current series had no any beneficial effects, in term of cost, lowering the CVP, blood loss and reduce the use of banked blood products, but resulted in significant lower Hb in perioperative period.

  17. Molecular characterization, distribution, and dynamics of hepatitis C virus genotypes in blood donors in Colombia.

    Science.gov (United States)

    Mora, Mónica Viviana Alvarado; Romano, Camila Malta; Gomes-Gouvêa, Michele Soares; Gutiérrez, Maria Fernanda; Carrilho, Flair José; Pinho, João Renato Rebello

    2010-11-01

    Hepatitis C virus (HCV) is a frequent cause of acute and chronic hepatitis and a leading cause for cirrhosis of the liver and hepatocellular carcinoma. HCV is classified in six major genotypes and more than 70 subtypes. In Colombian blood banks, serum samples were tested for anti-HCV antibodies using a third-generation ELISA. The aim of this study was to characterize the viral sequences in plasma of 184 volunteer blood donors who attended the "Banco Nacional de Sangre de la Cruz Roja Colombiana," Bogotá, Colombia. Three different HCV genomic regions were amplified by nested PCR. The first of these was a segment of 180 bp of the 5'UTR region to confirm the previous diagnosis by ELISA. From those that were positive to the 5'UTR region, two further segments were amplified for genotyping and subtyping by phylogenetic analysis: a segment of 380 bp from the NS5B region; and a segment of 391 bp from the E1 region. The distribution of HCV subtypes was: 1b (82.8%), 1a (5.7%), 2a (5.7%), 2b (2.8%), and 3a (2.8%). By applying Bayesian Markov chain Monte Carlo simulation, it was estimated that HCV-1b was introduced into Bogotá around 1950. Also, this subtype spread at an exponential rate between about 1970 to about 1990, after which transmission of HCV was reduced by anti-HCV testing of this population. Among Colombian blood donors, HCV genotype 1b is the most frequent genotype, especially in large urban conglomerates such as Bogotá, as is the case in other South American countries.

  18. 互助献血与无偿献血血清学标志物对比分析%The analysis of serological markers among mutual blood donors and voluntary blood donors

    Institute of Scientific and Technical Information of China (English)

    陈志忠; 张欣; 陈尚良; 廖扬勋; 梁洁贞; 陈超红; 陆倩文

    2014-01-01

    目的:对互助献血和无偿献血的血清学指标进行分析对比,研究探讨互助献血的风险。方法2006年1月至2012年12月,对符合献血条件的1834例互助献血者以及217323例无偿献血者进行了血液采集和留取 ETDA 抗凝样本,进行 ALT、HBsAg、Anti-HCV、Anti-HIV、梅毒共5个项目的初复检。结果互助献血(8.67%)的总阳性率要高于无偿献血组(6.31%),但两组的ALT 阳性率差异无统计学意义,互助献血组的HBsAg、Anti-HCV、梅毒均高于无偿献血组。结论无偿献血群体的血清学安全指标优于互助献血组,是血源性传播疾病较低的低危人群,应该是血液供应的主要来源群体;对互助献血采取合理的干预措施后,能确保其安全指数与无偿献血等同,可作为无偿献血的有益补充。%Objectives To analyze the epidemiology of viral markers and ALT among volunteer and replacement donors, and to reevaluate the risk of mutual blood donors. Methods From 2006, January to 2012, December, replacement and voluntary donors were recruited by hospital and center blood station respectively, and all the donors were screened HBsAg, anti-HCV, anti-HIV (1 + 2), syphilis and ALT in center blood station twice. Results In 217 323 volunteers and 1 834 replacement donors, the total seroprevalence was higher in replacement group than in voluntary group (8.67% vs 6.31%), but the prevalence of ALT were not significantly differences. The prevalence of HBsAg, anti-HCV and syphilis were higher in replacement group than in voluntary group. Conclusions Generally, volunteers cloud provide more viral safety than replacement donors, who should be the main source of blood supply. But with appropriate intervention measures, such as pre-donor screening and other donor selection policy, replacement donors and voluntary donors provide a similar level of viral safety.

  19. Lymphocyte subset reference intervals in blood donors from northeastern Brazil

    Directory of Open Access Journals (Sweden)

    ALEX J.L. TORRES

    2015-06-01

    Full Text Available The reference intervals for leukocytes and lymphocytes currently used by most clinical laboratories present limitations as they are primarily derived from individuals of North American and European origin. The objective this study was to determine reference values for peripheral blood B lymphocytes, T lymphocyte subsets (CD4+, CD8+, naïve, memory, regulatory, TCRαβ and TCRγδ+ and NK cells from blood donors in Salvador-Bahia, Brazil. Results: The proportion of included male subjects was 73.7% and the median ages of males (34 and females (35 were found to be similar. Absolute counts total lymphocytes subsets to both gender was 1,956 (1,060-4,186 cells and relative values 34%. The T CD4+ and T CD8+ lymphocytes relative values was 51% (20-62 and 24% (9-28, respectively. The most statistically significant finding observed was a higher percentage of B lymphocytes (p=0.03 in females. Commonly cited subset reference intervals were found to be consistent with values in several populations from different geographic areas.

  20. Legionella pneumophila Seropositivity-Associated Factors in Latvian Blood Donors

    Directory of Open Access Journals (Sweden)

    Olga Valciņa

    2015-12-01

    Full Text Available Continuous environmental exposure of humans to Legionella may induce immune responses and generation of antibodies. The aim of this study was to investigate the seroprevalence of Legionella pneumophila serogroups (SG 1–6 in the general healthy population and identify the associated host-related and environmental risk factors. L. pneumophila SG 1–6 seroprevalence among a total of 2007 blood samples collected from healthy donors was 4.8%. Seroprevalence was higher in women (5.9% than men (3.3% and in areas with a larger number of inhabitants, ranging from 3.5% in rural regions to 6.8% in the capital, Riga. Blood samples from inhabitants of apartment buildings tested positive for L. pneumophila in more cases (5.8% compared to those from inhabitants of single-family homes (2.7%. Residents of buildings with a municipal hot water supply system were more likely to be seropositive for L. pneumophila (OR = 3.16, 95% CI 1.26–7.91. Previous episodes of fever were additionally identified as a risk factor (OR = 2.42, 95% CI 1.43–4.1. In conclusion, centralized hot water supply, female gender and previous episodes of fever were determined as the main factors associated with L. pneumophila seropositivity in our study population.

  1. HEPATITIS B PREVALENCE AMONG BLOOD DONORS AT A TERTIARY CARE CENTRE IN MYSORE

    Directory of Open Access Journals (Sweden)

    Sreenivas

    2015-02-01

    Full Text Available BACKGROUND: Blood transfusion is an essential element of a health care system. Safety of blood transfusion is of extreme importance in order to avoid any severe morbidity and mortality in the patient. By screening donated blood units, we get a clue of the prevalence of those infections among donor pop ulations and consequently the safety of collected donations. It also gives us an idea of the prevalence of the Transfusion transmissible infections ( TTIs in the community. OBJECTIVES : To find out the sero - prevalence of TTIs namely HBV (Hepatitis B in the blood donor population at MMC&RI, Mysore. To stratify sero prevalence of TTIs based on the age and sex of the donor population . METHODOLOGY: The present study was carried out in the Blood Bank , Mysore Medical College and Research Institute , Mysore during the period from November 2012 to May 2014 among 14075 blood donors. All the samples were screened for hepatitis B surface antigen (HBsAg by ELISA method . RESULTS : Out of a total of 14075 blood donors , a total of 103 tested positive for TTIs . 94.08% were males and remaining 5.92% were females. A majority of donors were voluntary donors (85.79% and a majority of the donors were between the age group of 18 - 39 years (78.17%. The prevalence rate of HBV in blood donors was 0.73%. The seroprevale nce in voluntary donors was 0.57% and in replacement donors was 1.75 % respectively. CONCLUSION : Voluntary blood donation is safe, compared to replacement as high prevalence of Hepatitis B is observed in replacement donors.

  2. Prevalence and genotyping of hepatitis C virus in blood donors in the state of Pará, Northern Brazil

    Directory of Open Access Journals (Sweden)

    Aldemir B Oliveira-Filho

    2010-02-01

    Full Text Available Given the scarcity of epidemiological information on hepatitis C virus (HCV infection in Northern Brazil, we determined the prevalence and genotypic frequency in blood donors in the state of Pará (PA. Blood samples from all of the blood donors at the Fundação HEMOPA (blood bank of PA from 2004-2006 were screened for the presence of antibodies to anti-HCV and samples seroreactive to anti-HCV were further tested for HCV RNA using real-time PCR. In total, 116 HCV-RNA samples were genotyped, based on maximum likelihood phylogenetic analyses, using BioEdit, Modelgenerator, PHYML and FigTree software. The population consisted of 242,726 volunteers who donated blood from 2004-2006; the most common subgroup was males between the ages of 18-29 years old (37.30%. Within the whole group, 1,112 blood donors (0.46% had indeterminate or positive serology; among these, 28.78% were males whose ages ranged from 18-29 years. A diagnosis of chronic HCV infection was confirmed for 304 donors (60.20% males; 66.45% were 30-49 years old, resulting in a prevalence of HCV RNA in 0.13% of the samples (304 of 242,726. HCV genotyping revealed a high frequency of genotype 1 (108/116 followed by genotype 3 (8/116. This study found HCV infection to be relatively infrequent in PA; genotype 1 was most commonly isolated. This information can help guide prevention and control policies aimed at efficient diagnosis and control measures.

  3. An update of blood donor recruitment and retention in Hong Kong

    Directory of Open Access Journals (Sweden)

    Lee C

    2008-01-01

    Full Text Available A healthy blood donor pool has to be well maintained in order to achieve self sufficiency in blood supply. Not only should new and young donors should be attracted and recruited into the pool so as to compensate the loss from drop out and deferred donors. At the same time, previous donors should be also actively retained to ensure they can come regularly. The status of donor recruitment and retention in Hong Kong is reviewed here to highlight the current difficulties in coping with increasing blood demand from an ageing population, stringent donor eligibility criteria and quality requirement in the blood collection. With a systemic analysis of the donation pattern, proposal is put forward to tackle the challenging problems.

  4. Trends in hepatitis B virus infection among blood donors in Kelantan, Malaysia: a retrospective study.

    Science.gov (United States)

    Yousuf, R; Rapiaah, M; Ahmed, S A; Rosline, H; Salam, A; Selamah, S; Roshan, T M

    2007-11-01

    The objective of this study was to determine the prevalence and trends in hepatitis B infection among blood donors attending the Transfusion Medicine Unit at the Hospital Universiti Sains Malaysia, Kelantan, Malaysia. A retrospective study was carried out by reviewing the results of HBsAg among blood donors for the years 2000 to 2004. During this period, 44,658 blood donors were studied. We noted that there was a significant difference in the prevalence of hepatitis B infection between regular and first time donors. There was also a decreasing trend noticed in both study groups. The mean prevalence was significantly different between first time (1.83%) and regular donors (0.45%) (p < 0.005). There is a need to improve public awareness programs to lower the incidence of hepatitis B infection in the general population and consequently first time blood donors. Future studies are also required to determine the trends and outcomes of these programs.

  5. Prevalence of HIV and VDRL seropositivity in blood donors of Delhi.

    Science.gov (United States)

    Singh, Bharat; Verma, Monika; Kotru, Mrinalini; Verma, Karttikaye; Batra, Madhu

    2005-09-01

    Blood transfusion has been the transmission mechanism in 15 per cent of total patients infected with human immunodeficiency virus (HIV). A few reports are available regarding the trend of HIV seropositivity in northern India. Prevalence of VDRL (venereal disease research laboratory) reactivity varies from 0.8-15 per cent in blood donors. We present data on the prevalence and trends of infection with HIV 1 and 2 and VDRL reactivity in blood donors in Delhi. Between 2000-2002, a total of 76089 (voluntary and replacement) donors were screened. Majority (82.4%) were replacement donors. Seropositivity for HIV and VDRL was seen in 0.54 and 2.6 per cent of donors respectively. The percentage of seropositivity for HIV and VDRL was significantly higher in replacement donors (P<0.001). It is suggested that extensive donor selection and a voluntary donor service would reduce the number of infectious donors significantly. Non-renumerated repeat voluntary donor services are urgently required to lower the prevalence of transmissible infections. While the need to change to a voluntary donor service and devising effective donor screening cannot be over emphasized, there is also a need to mandate HIV antigen detection in India.

  6. Seroprevalence of Human T-Cell Lymphotropic Virus-1/2 in Blood Donors in Northern Pakistan: Implications for Blood Donor Screening.

    Science.gov (United States)

    Niazi, Saifullah Khan; Bhatti, Farhat Abbas; Salamat, Nuzhat

    2015-12-01

    To determine the seroprevalence of Human T-cell Lymphotropic Virus-1/2 (HTLV-1/2) in blood donors in Northern Pakistan. Descriptive study. Armed Forces Institute of Transfusion, Rawalpindi, from July to August 2013. A total of 2100 blood donors were screened for anti-HTLV-1/2 antibodies during the study period, in a pool of six, on a highly sensitive, Chemiluminiscent Microparticle Immunoassay (CMIA) based system. The screening test-reactive donors were recalled, counseled and interviewed, and a fresh sample was obtained for confirmatory testing. Confirmation was performed using additional immunoassays including Line Immunoassay (LIA); with additional testing for HTLV-1 pvDNAPCR. Frequency and percentages were determined. Four donors (0.19%) were repeatedly screening test-reactive and were subsequently confirmed to be HTLV-1 infected by line immunoassay and HTLV-1 pvDNAPCR. All four donors were male with mean age of 27 ± 6.27 years. Two (50%) of the positive donors gave history of Multiple Sexual Partners (MSP). HTLV-1 seroprevalence in Northern Pakistan blood donors was determined to be 0.19%. Large scale studies, including the cost effectiveness of screening blood donations for anti-HTLV-1/2 in Pakistan, are recommended.

  7. A novel strategy for screening blood donors for syphilis at Komfo Anokye Teaching Hospital, Ghana

    DEFF Research Database (Denmark)

    Sarkodie, F; Ullum, H; Owusu-Dabo, E

    2016-01-01

    OBJECTIVE: To implement and describe a novel syphilis screening strategy for blood donors. BACKGROUND: The seroprevalence of syphilis in blood donors is often high in low- and middle-income countries (LMIC) although the proportion of infectious donations is probably low. Syphilis screening may...... to a positive syphilis screen. CONCLUSION: In other LMIC, this novel strategy can contribute to improving blood safety without jeopardising blood supply....

  8. Infectivity of blood products from donors with occult hepatitis B virus infection

    DEFF Research Database (Denmark)

    Allain, Jean-Pierre; Mihaljevic, Ivanka; Gonzalez-Fraile, Maria Isabel

    2013-01-01

    BACKGROUND: Occult hepatitis B virus (HBV) infection (OBI) is identified in 1:1000 to 1:50,000 European blood donations. This study intended to determine the infectivity of blood products from OBI donors. STUDY DESIGN AND METHODS: Recipients of previous donations from OBI donors were investigated...

  9. ABO/RH DISTRIBUTION PATTERN AND PREVALENCE OF IRREGULAR ANTIBODIES IN HEALTHY BLOOD DONORS OF UTTARAKHAND

    Directory of Open Access Journals (Sweden)

    Brijesh Thakur

    2016-07-01

    Full Text Available BACKGROUND Blood group plays a vital role in transfusion safety, understanding genetics, inheritance pattern and disease susceptibility. This study is aimed to determine distribution pattern of ABO and Rh blood group, incidence and identification of irregular antibodies among blood donors of Uttarakhand. METHOD ABO/Rh blood grouping was performed by test tube agglutination method (both cell and serum grouping using antisera A, B and Rh from Tulip and Orthodiagnostics. Donors’ plasma were screened for irregular antibodies by indirect antiglobulin technique using Bio-Rad ID-Card “LISS/Coombs” and test cell reagents “ID-DiaCell” via gel centrifugation. RESULTS Out of total 4573 included blood donors, 88.2% were males. The maximum blood donors (77.6% were in 18-25 years of age group. The commonest ABO blood group present was B (36.1% followed by O (29.1%, A (24.8% and AB (10% in blood donors; while in Rhesus system, 95.0% donors were Rh-positive and 5.0% donors were Rh negative. Screening of donors’ plasma did not reveal any detectable irregular antibody. CONCLUSION The study has a significant implication in suggesting the distribution pattern of ABO/Rh blood group typing in the region, but routine irregular antibody screening in healthy blood donors may not be fruitful in this region keeping cost effectiveness and absence of previous sensitization in mind

  10. Plasma selenium status in a group of Australian blood donors and fresh blood components.

    Science.gov (United States)

    McDonald, Charles; Colebourne, Kathryn; Faddy, Helen M; Flower, Robert; Fraser, John F

    2013-10-01

    The purpose of this study was to assess plasma selenium levels in an Australian blood donor population and measure extra-cellular selenium levels in fresh manufactured blood components. Selenium levels were measured using graphite furnace atomic absorption spectrometry with Zeeman background correction. The mean plasma selenium level in healthy plasmapharesis donors was 85.6±0.5 μg/L and a regional difference was observed between donors in South East Queensland and Far North Queensland. Although participants had selenium levels within the normal range (55.3-110.5 μg/L), 88.5% had levels below 100 μg/L, a level that has been associated with sub-optimal activity of the antioxidant enzyme glutathione peroxidase (GPx). Extra-cellular selenium levels in clinical fresh frozen plasma (cFFP) and apheresis-derived platelets (APH Plt) were within the normal range. Packed red blood cells (PRBC) and pooled buffy coat-derived platelets (BC Plt) had levels at the lower limit of detection, which may have clinical implications to the massively transfused patient.

  11. Volunteer Donors' Redonation Willingness and Its Influencing Factors%无偿献血者再次献血意愿调查及影响因素分析

    Institute of Scientific and Technical Information of China (English)

    叶丽霞; 马小芳; 李莉; 古媛霞; 何丹单; 叶艺菲; 莫培娟

    2011-01-01

    目的 探讨无偿献血者(下称献血者)再次献血的意愿及其影响因素,从而提出针对性对策,提高再次献血者比例,建立稳定的献血者队伍.方法 采用自制问卷对2009年1-12月进行无偿献血的献血者891例进行调查,内容包括再次献血的意愿及其影响因素.结果 69.8%(622/891)的献血者表示愿意再次献血,不愿意或不清楚的占30.2%(269/891);献血服务互动质量是影响献血者再次献血的主要因素,其次是献血宣传和献血者献血过程的感受.结论 献血服务、献血宣传、献血者献血过程的感受等是影响献血者再次献血的主要因素.要提高献血者再次献血的比例,应通过提高献血服务互动质量,提高献血服务的信赖度和采供血人员的专业素质,加快献血知识以及相关政策的宣传力度和加强对采供血机构的监督与管理等来促进无偿献血的持续发展.%Objective To analyze volunteer donors' willingness to take a second donation and factors influencing their choices,thereby to propose precautionary measures to increase the number of regular donors and establish a stable team of volunteer donors.Methods A self-designed questionnaire was applied to conduct a survey from January to December 2009 among 891 volunteer donors. Results A total of 622 donors showed willingness to continue blood donation, accounting for 69.8%, while 30.2% (269/891) did not or did not suggest definite determination to take a second attempt. The interaction service quality during the blood donaion was the primary element affecting donors' decision, while the publicity of blood donation and donors' own feelings during the donating process serve as the followed impotant elements. Conclusion Blood donation service, blood donation promotion volunteer donors' experiences are the major factors that influence people's choice of a second donation. In order to boost the proportion of second blood donation, some measures such as

  12. Determination of hepatitis C virus genotypes among blood donors in Ahvaz, Iran

    Directory of Open Access Journals (Sweden)

    Farshadpour F

    2010-01-01

    Full Text Available This study aims to determine the genotypes of hepatitis C virus (HCV among blood donors at Ahvaz Blood Transfusion Centre. Blood samples were taken from 2376 blood donors - 1795 (75.54% male and 581(24.45% female - who referred to Ahvaz Blood Transfusion Centre during 2007-2008. Detection of anti-HCV antibody for all the donors was carried out by ELISA and the confirmatory RIBA tests. HCV RT-PCR followed by RFLP test was carried out for anti-HCV positive samples. Out of 2376 blood donors, only 55 (2.3% male donors showed to be positive for HCV antibody by ELISA and RIBA tests out of which 45(1.8% donors were positive for RT-PCR test. Female donors were negative for HCV antibody. The result of HCV genotyping by RFLP test showed 24 (53.3% for 1a, 17 (37.7% for 3a (a and 4 (8.8% for 3a (b genotypes respectively. In conclusion, high prevalence of 53.3% HCV 1a genotype was observed among blood donors in Ahvaz city.

  13. Evaluation of the TEG® platelet mapping™ assay in blood donors

    Directory of Open Access Journals (Sweden)

    Steinbrüchel Daniel A

    2007-02-01

    Full Text Available Abstract Background Monitoring of antiplatelet therapy in patients at cardiovascular risk is difficult because existing platelet function tests are too sophisticated for clinical routine. The whole blood TEG® Platelet Mapping™ assay measures clot strength as maximal amplitude (MA and enables for quantification of platelet function, including the contribution of the adenosine diphosphate (ADP and thromboxane A2 (TxA2 receptors to clot formation. Methods In 43 healthy blood donors, the analytical (CVa and inter-individual variability (CVg of the TEG® Platelet Mapping™ assay were determined together with platelet receptor inhibition in response to arachidonic acid (AA and ADP. Results The CVa of the assay for maximal platelet contribution to clot strength (MAThrombin was 3.5%, for the fibrin contribution to clot strength (MAFibrin 5.2%, for MAAA 4.5% and for MAADP it was 6.6%. The MAThrombin CVg was 2.8%, MAFibrin 4.7%, MAAA 6.6% and for MAADP it was 26.2%. Females had a higher MAThrombin compared to males (62.8 vs. 58.4 mm, p = 0.005. The platelet TxA2 receptor inhibition was 1.2% (range 0–10% and lower than for the ADP receptor (18.6% (0–58%; p Conclusion The high variability in ADP receptor inhibition may explain both the differences in response to ADP receptor inhibitor therapy and why major bleeding sometimes develops during surgery in patients not treated with ADP receptor inhibitors. An analytical variation of ~5 % for the TEG® enables, however, for routine monitoring of the variability in ADP receptor inhibition and of antiplatelet therapy.

  14. Antibody to histo-blood group A antigen neutralizes HIV produced by lymphocytes from blood group A donors but not from blood group B or O donors

    DEFF Research Database (Denmark)

    Arendrup, M; Hansen, J E; Clausen, H

    1991-01-01

    Three virus isolates HTLV-IIIB/lyA, HTLV-IIIB/lyB and HTLV-IIIB/lyO, obtained by passaging and propagating the HTLV-IIIB/H9 isolate in three separate cultures of mixed peripheral blood mononuclear cells (PBMC) from donors of blood type A, B or O, respectively, were tested for susceptibility...... for virus neutralization by the monoclonal antibody (MAb) AH16 directed against the blood group A epitope. MAb AH16 was previously shown to inhibit cell-free virus infection using HTLV-IIIB propagated in H9 cells. AH16 showed a concentration-dependent inhibition of the HTLV-IIIB/lyA isolate but did...... not inhibit the HTLV-IIIB/lyB or the HTLV-IIIB/lyO isolate. Specificity of the MAb-mediated inhibition was shown using A-antigen (tetrasaccharide). Thus, HIV infection of PBMC from donors with blood type A appears to induce expression of host-cell-encoded carbohydrate blood group A epitope on HIV which can...

  15. Antibody to histo-blood group A antigen neutralizes HIV produced by lymphocytes from blood group A donors but not from blood group B or O donors

    DEFF Research Database (Denmark)

    Arendrup, M; Hansen, J E; Clausen, H;

    1991-01-01

    for virus neutralization by the monoclonal antibody (MAb) AH16 directed against the blood group A epitope. MAb AH16 was previously shown to inhibit cell-free virus infection using HTLV-IIIB propagated in H9 cells. AH16 showed a concentration-dependent inhibition of the HTLV-IIIB/lyA isolate but did...... not inhibit the HTLV-IIIB/lyB or the HTLV-IIIB/lyO isolate. Specificity of the MAb-mediated inhibition was shown using A-antigen (tetrasaccharide). Thus, HIV infection of PBMC from donors with blood type A appears to induce expression of host-cell-encoded carbohydrate blood group A epitope on HIV which can......Three virus isolates HTLV-IIIB/lyA, HTLV-IIIB/lyB and HTLV-IIIB/lyO, obtained by passaging and propagating the HTLV-IIIB/H9 isolate in three separate cultures of mixed peripheral blood mononuclear cells (PBMC) from donors of blood type A, B or O, respectively, were tested for susceptibility...

  16. Elevated red blood cell 2,3-diphosphoglycerate levels in black blood donors.

    Science.gov (United States)

    Wallas, C H

    1978-01-01

    Mean levels of 2,3-diphosphoglycerate (DPG) were significantly increased in erythrocytes (RBC) from 43 nonanemic black blood donors (4.80 +/- 0.06 micromoles/l RBC) compared with 22 white donors 4.47 +/- 0.08 micromoles/l RBCs from eight of the 12 black donors with DPG levels greater than 5 micromoles/l RBC. Although a potentially hemolytic disorder could be defined in four (AS hemoglobin, beta-Thalassemia minor, G6PD deficiency), reticulocyte counts were normal. However, when RBCs from the subgroup were compared to RBCs from an additional 25 unselected white donors, the following suggested an abnormally large population of young RBCs in the subgroup: 1) normal or elevated RBC-ATP with normal serum phosphate level; 2) significantly increased activities of RBC age-dependent enzymes hexokinase (p less than 0.02), pyruvate kinase (p less than 0.05), and glutamicoxaloacetic transaminase (p less than 0.01), with normal activity of phosphoglycerate kinase, an age-independent enzyme; 3) decreased dense (older) RBCs as determined by sedimentation in phthalate esters. Since DPG is increased in young RBCs and falls as the RBC ages, loss of older relatively DPG depleted RBCs due to shortened survival could account for the elevated DPG levels seen in the subgroup.

  17. Seroepidemiology of infection with Toxoplasma gondii in healthy blood donors of Durango, Mexico

    Science.gov (United States)

    Alvarado-Esquivel, Cosme; Mercado-Suarez, Miguel Francisco; Rodríguez-Briones, Alfredo; Fallad-Torres, Laura; Ayala-Ayala, Julio Octavio; Nevarez-Piedra, Luis Jorge; Duran-Morales, Ehecatl; Estrada-Martínez, Sergio; Liesenfeld, Oliver; Márquez-Conde, José Ángel; Martínez-García, Sergio Arturo

    2007-01-01

    Background Toxoplasma gondii (T. gondii) infection in blood donors could represent a risk for transmission in blood recipients. There is scarce information about the epidemiology of T. gondii infection in blood donors in Mexico. Therefore, we sought to determine the prevalence of T. gondii infection and associated socio-demographic and behavioral characteristics in a population of healthy blood donors of Durango City, Mexico. Methods Four hundred and thirty two blood donors in two public blood banks of Durango City, Mexico were examined for T. gondii infection between August to September 2006. Blood donors were tested for anti-T. gondii IgG and IgM antibodies by using enzyme-linked immunoassays (Diagnostic Automation Inc., Calabasas, CA, USA). Socio-demographic and behavioral characteristics from each participant were also obtained. Results Thirty two (7.4%) of 432 blood donors had IgG anti-T. gondii antibodies. Eight (1.9%) of them had also IgM anti-T. gondii antibodies. Multivariate analysis using logic regression showed that T. gondii infection was associated with the presence of cats at home (adjusted OR = 3.81; 95% CI: 1.45–10.01). The age group of 45–60 years showed a significantly higher frequency of T. gondii infection than the group of 25–34 years (p = 0.02). Blood donors without education had a significantly higher frequency of infection (15.8%) than those with 13–19 years of education (4.5%) (p = 0.04). Other characteristics of blood donors including male gender, consumption of undercooked meat or blood transfusion did not show an association with infection. Conclusion The prevalence of T. gondii infection in healthy blood donors of Durango City, Mexico is lower than those reported in blood donors of south and central Mexico, and is one of the lowest reported in blood donors worldwide. T. gondii infection in our blood donors was most likely acquired by contact with cats. Prevalence of infection increased with age and decreased with educational

  18. Seroepidemiology of infection with Toxoplasma gondii in healthy blood donors of Durango, Mexico

    Directory of Open Access Journals (Sweden)

    Estrada-Martínez Sergio

    2007-07-01

    Full Text Available Abstract Background Toxoplasma gondii (T. gondii infection in blood donors could represent a risk for transmission in blood recipients. There is scarce information about the epidemiology of T. gondii infection in blood donors in Mexico. Therefore, we sought to determine the prevalence of T. gondii infection and associated socio-demographic and behavioral characteristics in a population of healthy blood donors of Durango City, Mexico. Methods Four hundred and thirty two blood donors in two public blood banks of Durango City, Mexico were examined for T. gondii infection between August to September 2006. Blood donors were tested for anti-T. gondii IgG and IgM antibodies by using enzyme-linked immunoassays (Diagnostic Automation Inc., Calabasas, CA, USA. Socio-demographic and behavioral characteristics from each participant were also obtained. Results Thirty two (7.4% of 432 blood donors had IgG anti-T. gondii antibodies. Eight (1.9% of them had also IgM anti-T. gondii antibodies. Multivariate analysis using logic regression showed that T. gondii infection was associated with the presence of cats at home (adjusted OR = 3.81; 95% CI: 1.45–10.01. The age group of 45–60 years showed a significantly higher frequency of T. gondii infection than the group of 25–34 years (p = 0.02. Blood donors without education had a significantly higher frequency of infection (15.8% than those with 13–19 years of education (4.5% (p = 0.04. Other characteristics of blood donors including male gender, consumption of undercooked meat or blood transfusion did not show an association with infection. Conclusion The prevalence of T. gondii infection in healthy blood donors of Durango City, Mexico is lower than those reported in blood donors of south and central Mexico, and is one of the lowest reported in blood donors worldwide. T. gondii infection in our blood donors was most likely acquired by contact with cats. Prevalence of infection increased with age and decreased

  19. Pre-birth selection of umbilical cord blood donors

    Science.gov (United States)

    Urciuoli, Patrizia; Passeri, Simona; Ceccarelli, Francesca; Luchetti, Barbara; Paolicchi, Aldo; Lapi, Simone; Nocchi, Francesca; Lamanna, Roberta; Iorio, Mariacarla; Vanacore, Renato; Mazzoni, Alessandro; Scatena, Fabrizio

    2010-01-01

    Background . The fact that only a small percentage of cord blood units (CBU) stored are actually used for transplantation contributes to raising the already high costs of their processing and cryopreservation. The identification of predictors allowing the early identification of suitable CBU would allow a reduction of costs for the collection, storage and characterisation of CBU with insufficient volume or cell numbers. In our bank we have adopted a cut-off value for using CBU of 8 × 108 nucleated cells and a volume ≥ 60 mL. Materials and methods In 365 banked CBU, we evaluated the correlation between neonatal/gestational parameters and laboratory data used to assess their quality. Results Biparietal diameter (BPD) and abdominal circumference were significantly and positively correlated with CBU volume (r2=0.12, p=0.0011 and r2=0.092, p=0.0063, respectively). Receiver operating characteristic (ROC) analysis showed that both parameters can be used to identify CBU with insufficient volume (BPD: area under the curve 0.69, 95% CI=0.57–0.82, p=0.004; abdominal circumference: area under the curve 0.67, 95% CI=0.54–0.79, p<0.01). BPD and head circumference, but not abdominal circumference or femoral length, were positively correlated with white blood cell (WBC) count (r2=0.215, p=0.031, and r2=0.299, p=0.015, respectively). Abdominal circumference, but not BPD, head circumference or femoral length, was statistically significantly correlated with the number of CD34+ cells in the CBU. Weight at birth and placental weight were positively correlated with WBC count, blood volume, CD34+ cell count, total colony-forming units and burst-forming units. Conclusion . Pre-birth assessment of BPD might allow the selection of donors who would yield CBU of sufficient volume and WBC count and avoid the costs of collecting, transferring, storing and analysing CBU with a high probability of resulting unsuitable for transplantation. PMID:20104277

  20. Whole blood and apheresis donors in Quebec, Canada: Demographic differences and motivations to donate.

    Science.gov (United States)

    Charbonneau, Johanne; Cloutier, Marie-Soleil; Carrier, Élianne

    2015-12-01

    This study sought to compare demographics and donation motivations among plasma/platelet donors (PPDs) and whole blood donors (WBDs), in a voluntary and non-remunerated context. Motives to donate blood and demographic characteristics were collected through questionnaires completed by 795 WBDs and 473 PPDs. Comparison of WBDs and PPDs under chi-square tests showed that 17 out of 23 motivators were statistically different according to various demographic variables. These results demonstrate the existence of specific donor profiles both for WBDs and PPDs. Agencies should develop new recruitment strategies tailored to these donors, especially if they wish to convince WBDs to convert to apheresis donation.

  1. Design of a donor-driven data collection strategy for operational improvement of blood donation process.

    Science.gov (United States)

    Padman, R; Heuston, M; Ashley, S; Bhortake, A; Carey, R; Dua, S; Mihelic, M; Rajderkar, S; Saini, V

    2010-07-01

    Prior studies have noted that donor retention may be negatively impacted by the total time it takes to complete the blood donation process. To gain a comprehensive understanding of the blood donation process and examine opportunities for operational improvements, an educational partnership established between a blood bank and a university designed and implemented a donor-driven data collection strategy. A large amount of real-time, comprehensive, donor-reported data was collected as donors navigated the process, which has enabled a thorough analysis of the process and its potential improvements. In this paper, we describe the design and implementation efforts, examine the challenges in operationalizing a donor-driven data collection approach, and present insights and recommendations for its application in similar settings. © 2010 American Association of Blood Banks.

  2. The effect of the benzodiazepine antagonist flumazenil on regional cerebral blood flow in human volunteers

    DEFF Research Database (Denmark)

    Wolf, J; Friberg, L; Jensen, J

    1990-01-01

    The influence of the benzodiazepine antagonist flumazenil on regional cerebral blood flow (rCBF) was investigated in ten healthy, alert volunteers. The design was a randomized, placebo-controlled, double-blind, cross-over study. rCBF was measured by 133-Xe inhalation and single photon emission...

  3. Prevalence of transmissible blood infections among blood donors at the University of Maiducuri Teaching Hospital, Maiduguri, Nigeria.

    Science.gov (United States)

    Chikwem, J O; Mohammed, I; Okara, G C; Ukwandu, N C; Ola, T O

    1997-04-01

    Before the advent of the Acquired Immune Deficiency Syndrome (AIDS), many countries of the world transfused blood without seriously considering the potential risks of transmission of infectious agents. Even after it was shown that the Human Immunodeficiency virus(HIV) could be transmitted through blood and blood products, many hospitals and clinics in Nigeria still continue to transfuse unscreened blood. This study was therefore initiated to highlight the risks of transmitting infectious agents through blood transfusion and the category of infectious agents which could be transfused in blood in this area. A total of 364 healthy blood donors were counselled, bled and screened for HIV-1, HIV-2, HBsAg, Treponema pallidum, Plasmodium falciparum and microfilaria. The results show that the three most common infections transmissible through blood transfusion are Hepatitis B(14.9%), HIV-1 (5.8%) and P.falciparum (4.1%). Thirteen of the 364 blood donors (3.6%) and antibodies to T. pallidum. There were no donors with HIV-2 or filarial infection. Infection of donors by hepatitis B virus (HBV), T. pallidum and HIV-1 was not significant dependent on promiscuity, polygamy, previous blood transfusion or local surgery. However, there was a significant difference between donors with no risk factors and those with risk factors with regard to seroprevalence to HBV, T. pallidum and HIV-1 (p = 0.0053). The results confirm that transfusion of unscreened blood carries severe risks of transmitting serious infectious agents and that is a need to enforce laws for transfusing blood in Nigeria. Meanwhile, in the absence of screening facilities, proper counselling of blood donors in order to ascertain their risk behaviour should be used to select donors and reduce this risk.

  4. Motivation in Italian whole blood donors and the role of commitment.

    Science.gov (United States)

    Bani, Marco; Strepparava, Maria Grazia

    2011-12-01

    The literature contains numerous reports on motivation in blood donors, although none of these are specific to blood donation in Italy and almost all of them focus on altruism and the desire to help others. Altruism is important, but a comprehensive analysis of donor motivation should examine all the factors affecting the decision to donate, including commitment to voluntary blood donor organizations. The aims of this paper are to verify if the motivational factors that influence the choice to donate blood in Italy are generally consistent with the findings from other countries reported in the literature and to focus on commitment to donor organizations as an additional factor. A sample of 895 whole blood donors completed a self-report questionnaire containing questions about: reasons for beginning to donate, people who influenced this choice, and level of commitment to voluntary blood donor organizations. The most frequently reported reasons for giving blood for the first time were "to help others" (56%), "influence of family/friends" (22%), and "social/moral obligation" (11.2%); commitment did not vary as a function of the leading motivation reported. Differences emerged between males, who more frequently reported having been influenced by parents and friends, and females, who referred more often to altruistic motives. The opportunity to check one's own state of health also played an important role (6.9%), especially for male donors. Overall, however, the decision to donate was primarily a personal choice (41.3%), although influence was also attributed to relatives (21.8%), friends (22.3%), and voluntary blood donor organizations (21.8%). The reported level of commitment to the donor organization was positively correlated with the number of total and annual donations made and number of new donors recruited.

  5. Real-Time Blood Donor Management Using Dashboards Based on Data Mining Models

    Directory of Open Access Journals (Sweden)

    Santhanam T

    2011-09-01

    Full Text Available This study uses data mining modeling techniques to examine the blood donor classification and extending this to facilitate the development of realtime blood donor management using dashboards with blood profile and geo-location data. This enables decision makers the ability to manage and plan the blood donation activities based on key metrics. This capability provides the ability to plan effective targeted blood donation campaigns. The scoring algorithm implemented for the dashboard also helps in the optimized deployment of budget resources and budget allocation determination for blood donation campaigns.

  6. A STUDY OF DISTRIBUTION OF ABO AND RH BLOOD GROUPS SYSTEM AMONG BLOOD DONORS AT A TERTIARY CARE HOSPITAL

    Directory of Open Access Journals (Sweden)

    Rajesh Kumar

    2015-02-01

    Full Text Available Up till now about 400red cells antigen have been identified. The majority are inherited by Mendelian fashion. The ABO and Rh blood group system was first to be identified and is most important for blood transfusion purposes. OBJECTIVE: This study was conducted to determine the frequency of ABO and Rhesus (Rh blood groups in a tertiary care teaching hospital in India. MATERIALS AND METHODS: A retrospective data based study was conducted at blood bank , Chirayu Medical College and Hospital, Bhopal, Madhya Pradesh, India over a period of four years. RESULTS: Study includes a record of 3188 (28.54% voluntary and 7982 (71.46% replacement donors attending blood bank from February 2011 to January 2015. Out of 11170, 10723(95.998% were male and 447(4.002% female donors. The most common blood group was found to be B in 4013 (35.927% donors followed by O in 3462 (30.994% donors , an in 2516 (22.524% donors and AB in 1179 (10.555% donors. Out of these, 10659(95.425% donors were Rh - positive while 511 ( 4.575 % were Rh - negative.

  7. Seroprevalence of infectious markers & their trends in blood donors in a hospital based blood bank in north India

    Directory of Open Access Journals (Sweden)

    R N Makroo

    2015-01-01

    Full Text Available Background & objectives: Hepatitis B virus (HBV, human immunodeficiency virus (HIV, hepatitis C virus (HCV and syphilis infections pose a great threat to blood safety. This study was undertaken to investigate the seroprevalence of serologic markers for transfusion transmitted infections (TTIs among blood donors at a hospital based blood centre in north India over a period of nine years. Methods: The results of serologic markers for TTIs (HBsAg, anti-HCV, anti-HIV and syphilis of all blood donations (both voluntary and replacement at our hospital from January 2005 to December 2013 were screened. Additional analysis was conducted to examine the prevalence trends associated with each of the positive marker. Results: The data of 180,477 donors [173,019 (95.86% males and 7,458 (4.13% females] were analyzed. Replacement donations [174,939 (96.93%] represented the majority whereas, only 5,538 (3.06% donations were from the voluntary donors. The risk of blood being reactive was three times higher in male donors when compared with the female donors. The risk of blood being reactive for one or more infectious markers was 2.1 times higher in replacement donors when compared with the voluntary donors. Seropositivity of HIV, HBsAg, HBcAb, syphilis showed a significant decreasing trend (P<0.05 while there was an increasing trend in HCV infection which was insignificant. Interpretation & conclusions: This study reflects that the risk of TTIs has been decreased over time with respect to HIV, HBV and syphilis, but the trends for HCV remains almost the same in blood donors. Blood transfusion remains a risk factor for the spread of blood-borne infections. Therefore, improvements are needed to strengthen both safety and availability of blood.

  8. TRENDS AND PREVALENCE OF INFECTIOUS MARKERS AMONG BLOOD DONORS FROM BLOOD BANK OF A TERTIARY CARE HOSPITAL

    Directory of Open Access Journals (Sweden)

    Sujata Kumbhar

    2015-01-01

    Full Text Available SUMMARY: Blood transfusion services form an integral part of health care; but simultaneously carries the risk of transmission of transfusion transmissible infections. We conducted a 5yr retrospective cross sectional study to estimate the prevalence of various infectious markers in the blood donors which was found to be 2.04%. INTRODUCTION : Blood safety is a major concern among health care personnel. We undertook this study to assess the prevalence of transfusion transmissible infections (TTI's namely - HIV, Hepatitis C, Syphilis and Malaria among blood donors from blood bank of a tertiary care hospital, in Western Maharashtra, India. METHODS : A total of 21,293 blood units were collected from donors. All blood units were screened for HIV, HBs Ag and HCV using ELISA. Test for syphilis was done by Rapid Plasma Reagin card test and peripheral smear examination was done to detect malarial parasite. RESULTS : A total of 21,293 blood donors were tested, of which 19,940 (93.65% were voluntary donors and 1,353 (6.35% were replacement donors. The highest seroprevalence observed was for HBs Ag (1.55% followed by HIV (0.38%, HCV (0.08% and Syphilis (0.02%. No donor was found to be positive for malaria parasite. CONCLUSION : Strategies need to be implemented to improve donor selection, using highly sensitive and specific screening tests and a better structured voluntary donation system. Nucleic acid amplification test would help to detect donors in window period for HIV infection. In view of high prevalence, effective community based programs and health education with emphasis on sexually transmitted diseases may prove helpful to decrease the seroprevalence.

  9. Frequency and Distribution of Blood Groups in Blood Donors in Western Ahmedabad AND#8211; A Hospital Based Study

    Directory of Open Access Journals (Sweden)

    Patel Piyush A

    2012-04-01

    Full Text Available Background: Up till now about 400 red cells antigen have been identified. The majority are inherited by Mendelian fashion. The ABO and Rhesus (Rh blood group system are most important for blood transfusion purposes, parental testing, legal medicine and in population genetic study. Objective: This study was conducted to determine and compare the frequency of ABO and Rh blood groups in blood donors in secondary care teaching hospital at Western Ahmedabad, Gujarat, India. Materials and Methods: A retrospective study was conducted at Blood bank, GMERS Medical College, Sola, Ahmedabad over a period of seven years from 1st January 2005 to 31st December 2011. Blood group of the blood donors was determined by commercially available standard monoclonal antisera by test tube agglutination technique. Results & conclusion: Out of 5316 subjects, 5076 (95.48% were male and 240 (4.52% were female subjects. The commonest ABO blood group present was B (39.40 % followed by O (30.79 %, A (21.94 % and AB (7.86 % in blood donors; while in Rhesus system, 5053(95.05% donors were Rh-positive and 263(4.95% donors were Rh-negative. The study has a significant implication regarding the inventory management of blood bank and transfusion services for the patient admitted in our secondary care teaching hospital. [National J of Med Res 2012; 2(2.000: 202-206

  10. Prevalence of Syphilis among Blood and Stem Cell Donors in Saudi Arabia: An Institutional Experience

    Science.gov (United States)

    Elyamany, Ghaleb; Al amro, Mohamed; Pereira, Winston Costa; Alsuhaibani, Omar

    2016-01-01

    Introduction Syphilis is one of the known transfusion-transmissible infections and causes 100,000 deaths yearly, with around 90% of these deaths occurring in the developing world. Little data is available regarding the prevalence of syphilis among Saudi blood and stem cell donors. We conducted a survey on the incidence of syphilis among all blood and stem cell donors. Methods This study was conducted at the Prince Sultan Military Medical City in Riyadh, Saudi Arabia in the 10 years period data during 2006–2015. Data were analyzed about full history, physical examination, age, sex, weight, profession, marital status, number of the donations, data of last donation, having a relation who received blood transfusion, as well as the screening test results of the donated blood. We determined the seroprevalence of infection and compared by sex and other variable through frequency analysis, Chi square, Fisher, and prevalence ratios. Results Approximately 240,000 blood donors were screened and studied in the period of study. Most of the blood donors were male (98.3%) and 89% of them were citizens of Saudi Arabia. According to our findings, we estimated that, in the last 10 years, approximately 0.044% of all the blood donors were syphilis positive cases. No cases were detected as positive for syphilis among stem cell donors. Only 60 blood donors tested positive for syphilis. In addition, we studied 202 stem cell transplant donors during the same period, of which 59% were male and none texted positive for syphilis. Conclusions A concerted effort between the government, health care providers, regulatory bodies and accreditation agencies have all contributed in eliminating the risk of spreading syphilis among blood donors.

  11. Serum cadmium levels in a sample of blood donors in the Western Amazon, Brazil, 2010-2011

    Directory of Open Access Journals (Sweden)

    Andre Ricardo Maia da Costa de Faro

    2014-02-01

    Full Text Available A cross-sectional study was conducted to determine the distribution of serum cadmium (Cd levels in blood donors in Rio Branco, Acre State, Brazil. Blood samples were obtained from 922 volunteer blood donors from 18 to 65 years of age at the Hemoacre blood center in 2010-2011. Mean serum Cd was 0.37µg/L (95%CI: 0.33-0.41. Increased serum Cd was associated with lower schooling; individuals with less than five years of schooling showed a mean Cd of 0.61µg/L (95%CI: 0.34-0.89, compared to 0.34µg/L (95%CI: 0.28-0.40 among those with more than nine years of schooling. Mean serum Cd was three times higher among smokers. Smoking showed a positive association with Cd level, with an OR of 12.36 (95%CI: 7.70-19.84. Meanwhile, serum Cd was lower among individuals that regularly drank tea, as compared to non-tea drinkers. Serum Cd levels were mostly below the reference value (88.3% of participants. Mean serum Cd in the current study indicates that in general the population studied here is not exposed to worrisome Cd levels.

  12. A behavior model for blood donors and marketing strategies to retain and attract them

    Directory of Open Access Journals (Sweden)

    Covadonga Aldamiz-echevarria

    2014-06-01

    Full Text Available OBJECTIVE: analyze and propose a theoretical model that describes blood donor decisions to help staff working in blood banks (nurses and others in their efforts to capture and retain donors.METHODS: analysis of several studies on the motivations to give blood in Spain over the last six years, as well as past literature on the topic, the authors' experiences in the last 25 years in over 15 Non Governmental Organizations with different levels of responsibilities, their experiences as blood donors and the informal interviews developed during those 25 years.RESULTS: a model is proposed with different internal and external factors that influence blood donation, as well as the different stages of the decision-making process.CONCLUSION: the knowledge of the donation process permits the development of marketing strategies that help to increase donors and donations.

  13. HIV, HBV, HCV and T. pallidum infections among blood donors and Transfusion-related complications among recipients at the Laquintinie hospital in Douala, Cameroon.

    Science.gov (United States)

    Eboumbou Moukoko, Carole Else; Ngo Sack, Françoise; Essangui Same, Estelle Géraldine; Mbangue, Madeleine; Lehman, Léopold Gustave

    2014-02-12

    Transfusion-transmissible infections (TTIs) pose a major health risk in Cameroon given the high prevalence of such pathogens and increased demands for blood donations in the local communities. This study aims at establishing the prevalence of commonly encountered TTIs among blood donors and transfusion-related complications among recipients in an urban center of Cameroon. A total of 477 blood donors and 83 blood recipients were recruited by consecutive sampling at the Laquintinie Hospital in Douala (LHD), Cameroon. Serum samples from blood donors were tested by quantitative enzyme-linked immunosorbent assays (ELISA) and/or using various Rapid diagnostic test (RDT) for presence of Hepatits B (HBV) viral antigens, and antibodies to human immunodeficiency (HIV-1/2), Hepatits B (HCV) and Treponema pallidum. Recipient's medical records were also analyzed for possible transfusion-associated complications. The male/female sex ratio of the blood donors was 4/1 with a mean age of 30.2 (Sd = 8.3) years. Of all blood donors, 64/467 (13.7%) were infected by at least one of the four TTIs. Infected volunteer donors represented 8.3% while infected family donors comprised 14.3% of the donor population. The prevalence of HCV, HIV, HBV and T. pallidum were 1.3%, 1.8%, 3.5%, and 8.1%, respectively. More than half of the blood recipients were female (78.3%) and the mean age was 20.6 (SD = 16.1) years. The causes of severe anemia indicative of transfusion in recipients varied with wards (postpartum hemorrhage, caesarean section, uterine or cervical lacerations, abortions, urinary tract infections, severe malaria, vaso-occlusive attacks, wounds and gastrointestinal bleeding). The most frequent complications were chills and hematuria, which represented 46.1% of all observed complications. Other complications such as nausea, vomiting, jaundice, sudden diarrhea, anxiety, tachycardia, or hyperthermia were also found in recipients. Three cases of deaths occurred during the study

  14. Serial follow-up of repeat voluntary blood donors reactive for anti-HCV ELISA

    Directory of Open Access Journals (Sweden)

    Choudhury N

    2011-01-01

    Full Text Available Background : Voluntary non-remunerated repeat blood donors are perceived to be safer than the first time blood donors. This study was planned for follow-up of previous hepatitis C virus (HCV test results of anti-HCV enzyme-linked immunosorbent assay (ELISA reactive repeat blood donors. The aim was to suggest a protocol for re-entry of the blood donors who are confirmed HCV negative by nucleic acid test (NAT and recombinant immunoblot assay (RIBA. A group of repeat voluntary donors were followed retrospectively who became reactive on a cross sectional study and showed HCV reactivity while donating blood regularly. Material and Methods: A total of 51,023 voluntary non remunerated blood donors were screened for anti-HCV ELISA routinely. If anybody showed positivity, they were tested by two ELISA kits (screening and confirmatory and then confirmed infection status by NAT and or RIBA. The previous HCV test results of repeat donors reactive by anti-HCV ELISA were looked back from the records. Data of donors who were repeat reactive with single ELISA kit (in the present study were analyzed separately from those reactive with two ELISA kits (in the present study. Results: In this study, 140 (0.27% donors who were reactive by anti HCV ELISA were included. Out of them, 35 were repeat voluntary donors and 16 (11.43% were reactive with single ELISA kit. All 16 donors were reactive by single ELISA kit occasionally in previous donations. Their present ELISA positive donations were negative for HCV NAT and RIBA. A total of 19 (13.57% donors were reactive with two ELISA kits. In their previous donations, the donors who were reactive even once with two ELISA kits were consistently reactive by the same two ELISA kits in their next donations also. Conclusion: Donor sample reactive by only single ELISA kit may not be considered as infectious for disposal as they were negative by NAT and or RIBA. One time ELISA positivity was found probably due to ELISA kit

  15. Knowledge, attitudes, beliefs and motivations towards blood donations among blood donors in Lagos, Nigeria.

    Science.gov (United States)

    Olaiya, M A; Alakija, W; Ajala, A; Olatunji, R O

    2004-02-01

    Summary. interviewed with questionnaires on their knowledge, attitudes, beliefs and motivations about blood donations. It was found that a large number of them (92.9%) donated because of the benefits they will obtain from the hospital. Such benefits include antenatal registration (67.1%) and saving the lives of relations (25.8%). Even though many of the donors are educated (98.9%), majority of whom have university degrees (36.1%) and have heard about blood donation before, 52.4% of them believe they can contact human immunodeficiency virus (HIV) and/or hepatitis infection from blood donation. A good number (47.0%) are afraid of what they regard as side effects, such as weight loss (23.8%), sexual failure (5.9%), high blood pressure (5.2%), sudden death (3.3%), and convulsion (1.47%). About 41.0% prefers certificates as an incentive for donation, whereas 13.6% prefers money; less than 3% will like their names announced or published on the media and 2.58% will donate for nothing. It is recommended that an intensive blood donation campaign should be maintained. This will allow people to be well informed, turning the positive attitude of saving life through blood donation to a regular practice.

  16. Characteristics of donors who do or do not return to give blood and barriers to their return

    NARCIS (Netherlands)

    Wevers, A.; Wigboldus, D.H.J.; Kort, W.L.A.M.M. de; Baaren, R.B. van; Veldhuizen, I.J.T.

    2014-01-01

    Background In the Netherlands about 50% of whole blood donors return to give blood after an invitation to donate. This study aimed to investigate the characteristics of donor return behaviour and to gain insight into the barriers to blood donation reported by the donors themselves. Materials and

  17. Characteristics of donors who do or do not return to give blood and barriers to their return

    NARCIS (Netherlands)

    Wevers, A.; Wigboldus, D.H.J.; Kort, W.L.A.M.M. de; Baaren, R.B. van; Veldhuizen, I.J.T.

    2014-01-01

    Background In the Netherlands about 50% of whole blood donors return to give blood after an invitation to donate. This study aimed to investigate the characteristics of donor return behaviour and to gain insight into the barriers to blood donation reported by the donors themselves. Materials and met

  18. Prevalence of iron deficiency anaemia among blood donors in Sokoto, North Western, Nigeria

    Institute of Scientific and Technical Information of China (English)

    Buhari Hauwa Ali; Yeldu Mohammed Haruna; Erhabor Osaro; Imrana Sani; Abubakar Wase; Onuigwe Festus; Okwesili Augustine; Isaac Zama; Yakubu Abdulrahaman; Dallatu Kabiru

    2015-01-01

    Objective: To investigate the prevalence and socio-demographic factors associated with iron deficiency anaemia among blood donors in Sokoto, North Western, Nigeria using a combination of haemoglobin haematocrit and serum ferritin measurements. Methods: One hundred and fifty consecutively recruited whole blood donors, comprising of 148 (98.7%) family replacement donors and 2 (1.3%) voluntary non-remunerated donors aged 18-60 years and mean age 39±21 years constituted the subjects for this study. The full blood count was carried out using Mythic 22 CT fully automated haematology analyser (Orphee SA, Switzerland). Serum was tested for ferritin using a human ferritin enzyme immunoassay kit-ACCU Diag™ ELISA Ferritin kit (Diagnostic Automation/Cortez Diagnostic Inc. California, USA). Results: The prevalence of anaemia (haemoglobin<11.0 g/dL) was evident in 24 (16%) and iron deficiency anaemia (serum ferritin<12 ng/mL+haemoglobin<11 g/dL) in 5 (10%) of donors. The haemoglobin and ferritin levels was significantly lower among regular voluntary remunerated blood donors (13.50±0.00 and 34.88±0.00) compared to family replacement donors (14.10±2.40 and 74.12±45.20) respectively (P=0.01 and 0.05 respectively). The mean haemoglobin and ferritin level was compared among donors based on gender. The haemoglobin and ferritin was significantly higher among male donors (14.20±2.00, 78.02±49.10) compared to female donors (12.35±2.5 and 42.20±32.13) (P=0.01). The mean haemoglobin and ferritin level was compared among donors based on occupational groups. The haemoglobin and ferritin was significantly higher among civil servants compared to farmers and students (P=0.01). Conclusions: Iron deficiency anaemia is prevalent among blood donors in Sokoto, North Western, Nigeria. There is need to include routine ferritin in the blood donor testing protocol in the area to enable the diagnosis of donors with latent iron deficiency anaemia to facilitate iron supplementation for regular

  19. Prevalence of iron deficiency anaemia among blood donors in Sokoto, North Western, Nigeria

    Directory of Open Access Journals (Sweden)

    Buhari Hauwa Ali

    2015-04-01

    Full Text Available Objective: To investigate the prevalence and socio-demographic factors associated with iron deficiency anaemia among blood donors in Sokoto, North Western, Nigeria using a combination of haemoglobin haematocrit and serum ferritin measurements. Methods: One hundred and fifty consecutively recruited whole blood donors, comprising of 148 (98.7% family replacement donors and 2 (1.3% voluntary non-remunerated donors aged 18-60 years and mean age 39±21 years constituted the subjects for this study. The full blood count was carried out using Mythic 22 CT fully automated haematology analyser (Orphee SA, Switzerland. Serum was tested for ferritin using a human ferritin enzyme immunoassay kitACCU Diag™ ELISA Ferritin kit (Diagnostic Automation/Cortez Diagnostic Inc. California, USA. Results: The prevalence of anaemia (haemoglobin<11.0 g/dL was evident in 24 (16% and iron deficiency anaemia (serum ferritin<12 ng/mL+haemoglobin<11 g/dL in 5 (10% of donors. The haemoglobin and ferritin levels was significantly lower among regular voluntary remunerated blood donors (13.50±0.00 and 34.88±0.00 compared to family replacement donors (14.10±2.40 and 74.12±45.20 respectively (P=0.01 and 0.05 respectively. The mean haemoglobin and ferritin level was compared among donors based on gender. The haemoglobin and ferritin was significantly higher among male donors (14.20±2.00, 78.02±49.10 compared to female donors (12.35±2.5 and 42.20±32.13 (P=0.01. The mean haemoglobin and ferritin level was compared among donors based on occupational groups. The haemoglobin and ferritin was significantly higher among civil servants compared to farmers and students (P=0.01. Conclusions: Iron deficiency anaemia is prevalent among blood donors in Sokoto, North Western, Nigeria. There is need to include routine ferritin in the blood donor testing protocol in the area to enable the diagnosis of donors with latent iron deficiency anaemia to facilitate iron supplementation for

  20. Influence of postmortem time on the outcome of blood cultures among cadaveric tissue donors.

    Science.gov (United States)

    Saegeman, V; Verhaegen, J; Lismont, D; Verduyckt, B; De Rijdt, T; Ectors, N

    2009-02-01

    Tissue banks provide tissues of human cadaver donors for transplantation. The maximal time limit for tissue retrieval has been set at 24 h postmortem. This study aimed at evaluating the evidence for this limit from a microbiological point of view. The delay of growth in postmortem blood cultures, the identification of the species isolated and clinical/environmental factors were investigated among 100 potential tissue donors. No significant difference was found in the rate of donors with grown blood cultures within (25/65=38%) compared with after (24/65=37%) 24 h of death. Coagulase-negative staphylococci and gastro-intestinal microorganisms were isolated within and after 24 h of death. Two factors--antimicrobial therapy and "delay before body cooling"--were significantly inversely related with donors' blood culture results. From a microbiological point of view, there is no evidence for avoiding tissue retrieval among donors after 24 h of death.

  1. Donor Tracker: An Innovative Real-Time Tracking System for Blood ...

    African Journals Online (AJOL)

    User

    track blood donors in Mauritius and locate the nearest donor in cases of emergencies ... lifestyle makes computing increasingly mobile and ubiquitous today. ..... Figures 6.1 (a), 6.1 (b) and 6.1 (c) illustrates the steps for downloading the mobile.

  2. Two decades of risk factors and transfusion-transmissible infections in Dutch blood donors

    NARCIS (Netherlands)

    Slot, Ed; Janssen, Mart P.; Marijt-Van Der Kreek, Tanneke; Zaaijer, Hans L.; Van De Laar, Thijs J.

    2016-01-01

    BACKGROUND Risk behavior-based donor selection procedures are widely used to mitigate the risk of transfusion-transmissible infections (TTIs), but their effectiveness is disputed in countries with low residual risks of TTIs. STUDY DESIGN AND METHODS In 1995 to 2014, Dutch blood donors infected with

  3. Two decades of risk factors and transfusion-transmissible infections in Dutch blood donors

    NARCIS (Netherlands)

    Slot, Ed; Janssen, Mart P.; Marijt-Van Der Kreek, Tanneke; Zaaijer, Hans L.; Van De Laar, Thijs J.

    2016-01-01

    BACKGROUND Risk behavior-based donor selection procedures are widely used to mitigate the risk of transfusion-transmissible infections (TTIs), but their effectiveness is disputed in countries with low residual risks of TTIs. STUDY DESIGN AND METHODS In 1995 to 2014, Dutch blood donors infected with

  4. [Automated serial diagnosis of donor blood samples. Ergonomic and economic organization structure].

    Science.gov (United States)

    Stoll, T; Fischer-Fröhlich, C L; Mayer, G; Hanfland, P

    1990-01-01

    A comprehensive computer-aided administration-system for blood-donors is presented. Ciphered informations of barcode-labels allow the automatic and nevertheless selective pipetting of samples by pipetting-robots. Self-acting analysis-results are transferred to a host-computer in order to actualize a donor data-base.

  5. Red cell antigen prevalence predicted by molecular testing in ethnic groups of South Texas blood donors.

    Science.gov (United States)

    Aranda, Lorena I; Smith, Linda A; Jones, Scott; Beddard, Rachel

    2015-01-01

    Alloimmunization to red blood cell antigens is seen in patients receiving chronic blood transfusion. Knowing the prevalence of blood group antigens of the different ethnicities of South Texas donors can provide better management of rare blood inventory for patients in this geographical area. A total of 4369 blood donors were tested and analyzed for various antigens in the following blood group systems: ABO, Rh, Kell, Duffy, Kidd, MNS, Lutheran, Dombrock, Landsteiner-Wiener, Diego, Colton, and Scianna. Donors tested to be group 0 or A were serologically tested for the Rh (C, E, c, e) antigens. Those that tested as presumably R1R1, R2R2, or Ror were then genotyped. Donors constituted three major ethnicities: black (18.3%), Hispanic (36.3%), and Caucasian (41.1%); ethnicities comprised of Asian, American Indian, multiracial, and other accounted for the remaining donors (4.3%). The most likely common Rh phenotype for each ethnicity is as follows: black -Ror (44.4%), Hispanic -R1R1 (59.0%), and Caucasian -R1R1 (38.9%). The prevalence of Kell, Duffy, and Kidd blood group system antigens in black and Caucasian donors is comparable with published reports for the entire U.S. The black South Texas donor population had an 8.8 percent increase in prevalence of the Fy(a+b-) phenotype as compared with these published reports; the Hispanic South Texas donor population had a prevalence of 36.1 percent of the Fy(a+b-) phenotype. Regarding the Diego blood group system, the Hispanic donor population in South Texas had a prevalence of 93.5 percent for the Di(a-b+) phenotype as compared with published reports for the entire U.S. (>99.9%). The Hispanic population had a prevalence of 7.9 percent of donors testing as M-N+S-s+ as compared with 20.2 percent and 15.6 percent for black and Caucasian donors, respectively. This study helped us determine the prevalence of each of the blood group antigens in the South Texas donor population to establish and maintain adequate rare inventory of

  6. 75 FR 22813 - Guidance for Industry: Requalification Method for Reentry of Blood Donors Deferred Because of...

    Science.gov (United States)

    2010-04-30

    ...The Food and Drug Administration (FDA) is announcing the availability of a document entitled ``Guidance for Industry: Requalification Method for Reentry of Blood Donors Deferred Because of Reactive Test Results for Antibody to Hepatitis B Core Antigen (Anti- HBc),'' dated May 2010. The guidance document provides recommendations to establishments that collect Whole Blood or blood components......

  7. Asymptomatic malaria and associated factors among blood donors ...

    African Journals Online (AJOL)

    Dr.Mirambo

    falciparum which poses a risk for transmission to blood recipients including ... to an infected person's blood via blood transfusion, sharing needles or syringes .... in agreement with a previous report in Nakuru, Kenya, which had a similar ...

  8. Sero-prevalence of Human Cytomegalovirus among blood donors in Lahore, Pakistan

    Directory of Open Access Journals (Sweden)

    Chahat Batool Rizvi

    2015-08-01

    Full Text Available Background: Transfusion-transmitted cytomegalovirus (TT-CMV infection can cause severe illness and even death among immunocompromised patients; therefore, the spread of CMV through blood products should be prevented. To our knowledge, no study has been carried out in Pakistan to determine the seroprevalence of CMV in general population as well as among blood donors. The goal of this study was to determine CMV seropositivity among blood donors at the blood bank of INMOL Hospital, Lahore, Pakistan. Methods: A sero-epidemiological cross-sectional study was conducted. Sera from 91 blood donors were screened for CMV specific IgG antibodies by enzyme-linked immunosorbent assay (ELISA based kit. Results: The CMV-specific IgG antibodies were detected in 89 blood donors, which gave seroprevalence rate of 97.8%. The statistical analysis of results was done using pearson chi-square test and appeared non-significant with values 0.625 and 0.705 for different age groups and blood groups of donors. Conclusion: Because of high seroprevalence in this study area, an adequate supply of CMV seronegative blood is difficult to maintain. Therefore, we propose that the future strategies for the prevention of post-transfusion CMV infection in recipients should include the transfusion of leukoreduced blood products. Further a prospective study with much greater population can be done to identify major causative risk factors for such highest prevalence rate.

  9. Namibia's transition from whole blood-derived pooled platelets to single-donor apheresis platelet collections

    NARCIS (Netherlands)

    Pitman, John P.; Basavaraju, Sridhar V.; Shiraishi, Ray W.; Wilkinson, Robert; von Finckenstein, Bjorn; Lowrance, David W.; Marfin, Anthony A.; Postma, Maarten; Mataranyika, Mary; Smit Sibinga, Cees Th.

    2015-01-01

    BACKGROUNDFew African countries separate blood donations into components; however, demand for platelets (PLTs) is increasing as regional capacity to treat causes of thrombocytopenia, including chemotherapy, increases. Namibia introduced single-donor apheresis PLT collections in 2007 to increase PLT

  10. Impact of nucleic acid amplification test on screening of blood donors in Northern Pakistan.

    Science.gov (United States)

    Niazi, Saifullah Khan; Bhatti, Farhat Abbas; Salamat, Nuzhat; Ghani, Eijaz; Tayyab, Muhammad

    2015-07-01

    The Armed Forces Institute of Transfusion located in Rawalpindi, Northern Pakistan, acts as a regional blood center with more than 50,000 donations collected annually. Nucleic acid amplification testing (NAT) was introduced in our institution in September 2012 for screening all seronegative blood donors. The study was conducted from September 21, 2012, to September 20, 2013. Samples from the seronegative donors were run on cobas s 201 platform (Roche) in pools of six. Reactive donors were followed up for further confirmatory testing to rule out false-positive results. Viral load estimation was done for all NAT-reactive donors. After serologic screening of 56,772 blood donors, 2334 were found to be reactive; 719 (1.27%) were reactive for hepatitis B surface antigen, 1046 (1.84%) for antibody to hepatitis C virus (anti-HCV), 12 (0.02%) for antibody to human immunodeficiency virus, and 557 (0.98%) for syphilis antibodies. A total of 27 NAT-reactive donors were confirmed after testing 54,438 seronegative donors, with an overall NAT yield of one in 2016 donors: 23 for hepatitis B virus (HBV) DNA (HBV NAT yield, 1:2367) and four for HCV RNA (HCV NAT yield, 1:13,609). The residual risk after NAT implementation, calculated for the first-time blood donors, was 62.5 and 4.4 per million donors for HBV and HCV, respectively. NAT has improved the safety of blood products at our transfusion institution. Confirmation of NAT results must always be done either on follow-up samples or on samples from the retrieved frozen plasma bag. © 2015 AABB.

  11. Is current serologic RhD typing of blood donors sufficient for avoiding immunization of recipients?

    DEFF Research Database (Denmark)

    Krog, Grethe Risum; Clausen, Frederik Banch; Berkowicz, Adela

    2011-01-01

    Avoiding immunization with clinically important antibodies is a primary objective in transfusion medicine. Therefore, it is central to identify the extent of D antigens that escape routine RhD typing of blood donors and to improve methodology if necessary.......Avoiding immunization with clinically important antibodies is a primary objective in transfusion medicine. Therefore, it is central to identify the extent of D antigens that escape routine RhD typing of blood donors and to improve methodology if necessary....

  12. Frequency of thalassemia carrier and Hb variant and the quality of stored donor blood

    Directory of Open Access Journals (Sweden)

    Eva A. Maharani

    2015-01-01

    Full Text Available Background: This study was aimed to determine the frequency of thalassemia and Hb variant in blood donor. In addition, we also wanted to know the quality of blood from the donor up to seven days of storage, by calculating percentage of hemolysis in vitro.Methods: This cross-sectional study was conducted on 138 blood donor specimens at Red Cross Blood Centre Unit in Jakarta. All specimens were tested for thalassemia and Hb variant by complete blood count (CBC and Hb analysis with HPLC method and DNA analysis for the detection of α thalassemia carrier. To analyze the quality of stored blood, the calculation of hemolytic rate of red blood cells (RBCs on whole blood (WB was compared between the first and seventh days of storage.Results: Out of the 138 specimens, 5 samples (3.6% were diagnosed for α thalassemia carrier in which, one of them is co-inherited with ovalositosis hereditary (Southeast Asian Ovalositosis/SAO, 3 samples (2.2% for β thalassemia carrier, and 3 samples (2.2% for Hb E. Meanwhile, the hemolytic rates of RBCs on WB in first day and seven day of storage were below one percent.Conclusion: The frequency of thalassemia carrier and Hb variants in blood donors at Red Cross Blood Centre Unit in Jakarta was 8%. The quality of stored blood until seven day of storage was quite good.

  13. Assessing Acceptability of Short Message Service Based Interventions towards Becoming Future Voluntary Blood Donors

    Directory of Open Access Journals (Sweden)

    Sana Saleem

    2014-01-01

    Full Text Available All blood bank services, especially those of developing countries, face a major shortfall of blood donations due to lack of voluntary blood donors. Our study aims to evaluate the acceptability of Short Message Service based interventions towards becoming voluntary blood donors among medical university students of Karachi, Pakistan. Methods. A total of 350 medical students were approached in medical universities of Karachi, Pakistan, using a nonprobability convenient sampling technique. Data collectors administered a self-made questionnaire to each participant using an interview based format. All data was recorded and analyzed on SPSS 16. Results. 350 participants, having a mean age of 21.47 ± 1.36, were included in our study with 30.6% (107/350 being males and 69.4% (243/350 being females. 93.4% (327/350 of participants agreed that donating blood was healthy, but only 26% had donated blood in the past with 79.1% donating voluntarily. 65.7% (230/350 of the participants agreed to take part in Short Message Service based behavioral interventions to become voluntary blood donors with 69.7% (244/350 also agreeing that Short Message Service reminders will promote them to donate blood more often. Conclusion. With university students willing to become voluntary blood donors, Pakistani blood banks can carry out Short Message Service based interventions to encourage them to donate blood.

  14. Assessing Acceptability of Short Message Service Based Interventions towards Becoming Future Voluntary Blood Donors.

    Science.gov (United States)

    Saleem, Sana; Wasim, Anum; Sabih, Sidra; Khan, Ayisha Farooq; Rizvi, Madiha Hasan; Jillani, Umaima Ayesha; Syed, Mujtaba Jamal; Mumtaz, Madiha; Mumtaz, Yasmeen; Shehzad, Abdul Moid; Dawani, Om; Khan, Saima; Munir, Sheheryar; Asad, Nava; Kazi, Abdul Nafey

    2014-01-01

    All blood bank services, especially those of developing countries, face a major shortfall of blood donations due to lack of voluntary blood donors. Our study aims to evaluate the acceptability of Short Message Service based interventions towards becoming voluntary blood donors among medical university students of Karachi, Pakistan. Methods. A total of 350 medical students were approached in medical universities of Karachi, Pakistan, using a nonprobability convenient sampling technique. Data collectors administered a self-made questionnaire to each participant using an interview based format. All data was recorded and analyzed on SPSS 16. Results. 350 participants, having a mean age of 21.47 ± 1.36, were included in our study with 30.6% (107/350) being males and 69.4% (243/350) being females. 93.4% (327/350) of participants agreed that donating blood was healthy, but only 26% had donated blood in the past with 79.1% donating voluntarily. 65.7% (230/350) of the participants agreed to take part in Short Message Service based behavioral interventions to become voluntary blood donors with 69.7% (244/350) also agreeing that Short Message Service reminders will promote them to donate blood more often. Conclusion. With university students willing to become voluntary blood donors, Pakistani blood banks can carry out Short Message Service based interventions to encourage them to donate blood.

  15. Seroprevalence of HBV and HCV in blood donors: A study from regional blood transfusion services of Nepal

    Directory of Open Access Journals (Sweden)

    Tiwari B

    2010-01-01

    Full Text Available Background and Objective : Hepatitis B and hepatitis C are significant health problems that might involve the late sequel of liver cirrhosis and hepatocellular carcinoma. A high prevalence of hepatitis B virus (HBV and hepatitis C virus (HCV in blood donors poses an increased risk of window period transmission through blood transfusion. The present study aimed to know the seroprevalence of hepatitis B virus (HBV and hepatitis C virus (HCV among blood donors in regional blood transfusion services of Nepal. Materials and Methods: This was a retrospective study conducted among blood donors in Banke (5,211, Morang (5,351, and Kaski (5,995 blood transfusion services. Serum samples were tested for hepatitis B surface antigen (HBsAg and anti-HCV antibodies using rapid enzyme immunoassays. The donors information was collected via the donor record register through their respective blood transfusion services. The software "Winpepi ver 3.8" was used for statistical analysis. Results: The seroprevalence rate of HBV was highest in the Banke (1.2% followed by Biratnagar (0.87% and Kaski (0.35% (P < 0.0001. The seroprevalence of HCV was highest in the Morang (0.26% followed by Kaski (0.16% and Banke (0.11% (P > 0.05. The seroprevalence of HBV was significantly higher than HCV in all three blood transfusion services. The burden of HBV as well as HCV seems to be higher in male donors (P > 0.05. Conclusion: The study revealed that the seroprevalence of HBV was alarmingly higher in two of the three blood transfusion services. Implementation of community-based preventive measures and improved strategies for safe blood supply might prove useful to decrease the seroprevalence.

  16. West Nile virus lineage 2 infection in a blood donor from Vienna, Austria, August 2014.

    Science.gov (United States)

    Jungbauer, C; Hourfar, M K; Stiasny, K; Aberle, S W; Cadar, D; Schmidt-Chanasit, J; Mayr, W R

    2015-03-01

    Eastern Austria is neighbouring regions with ongoing West Nile virus (WNV) transmissions. Three human WNV infections had been diagnosed during the past decade in Austria. The Austrian Red Cross Blood Service (ARC-BS) started a first voluntary screening for WNV in blood donors from Eastern Austria by Nucleic Acid Testing (NAT) in June 2014. This is also the most extensive WNV surveillance programme in humans in Austria so far. In August 2014, one autochthonous WNV infection was detected in a blood donor from Vienna. By now, one in 67,800 whole blood donations was found to be positive for WNV RNA.

  17. Prevalence of glucose-6-phosphate dehydrogenase deficiency and sickle cell trait among blood donors in Riyadh

    Directory of Open Access Journals (Sweden)

    Alabdulaali Mohammed

    2010-01-01

    Full Text Available Background and Aims: Blood donation from glucose-6-phosphate dehydrogenase (G6PD-deficient and sickle cell trait (SCT donors might alter the quality of the donated blood during processing, storage or in the recipient′s circulatory system. The aim of this study was to determine the prevalence of G6PD deficiency and SCT among blood donors coming to King Khalid University Hospital (KKUH in Riyadh. It was also reviewed the benefits and risks of transfusing blood from these blood donors. Materials and Methods: This cross-sectional study was conducted on 1150 blood samples obtained from blood donors that presented to KKUH blood bank during the period April 2006 to May 2006. All samples were tested for Hb-S by solubility test, alkaline gel electrophoresis; and for G6PD deficiency, by fluorescent spot test. Results: Out of the 1150 donors, 23 (2% were diagnosed for SCT, 9 (0.78% for G6PD deficiency and 4 (0.35% for both conditions. Our prevalence of SCT and G6PD deficiency is higher than that of the general population of Riyadh. Conclusion: We recommend to screen all units for G6PD deficiency and sickle cell trait and to defer donations from donors with either of these conditions, unless if needed for special blood group compatibility, platelet apheresis or if these are likely to affect the blood bank inventory. If such blood is to be used, special precautions need to be undertaken to avoid complications in high-risk recipients.

  18. Viral metagenomics applied to blood donors and recipients at high risk for blood-borne infections

    Science.gov (United States)

    Sauvage, Virginie; Laperche, Syria; Cheval, Justine; Muth, Erika; Dubois, Myriam; Boizeau, Laure; Hébert, Charles; Lionnet, François; Lefrère, Jean-Jacques; Eloit, Marc

    2016-01-01

    Background Characterisation of human-associated viral communities is essential for epidemiological surveillance and to be able to anticipate new potential threats for blood transfusion safety. In high-resource countries, the risk of blood-borne agent transmission of well-known viruses (HBV, HCV, HIV and HTLV) is currently considered to be under control. However, other unknown or unsuspected viruses may be transmitted to recipients by blood-derived products. To investigate this, the virome of plasma from individuals at high risk for parenterally and sexually transmitted infections was analysed by high throughput sequencing (HTS). Materials and methods Purified nucleic acids from two pools of 50 samples from recipients of multiple transfusions, and three pools containing seven plasma samples from either HBV−, HCV− or HIV-infected blood donors, were submitted to HTS. Results Sequences from resident anelloviruses and HPgV were evidenced in all pools. HBV and HCV sequences were detected in pools containing 3.8×103 IU/mL of HBV-DNA and 1.7×105 IU/mL of HCV-RNA, respectively, whereas no HIV sequence was found in a pool of 150 copies/mL of HIV-RNA. This suggests a lack of sensitivity in HTS performance in detecting low levels of virus. In addition, this study identified other issues, including laboratory contaminants and the uncertainty of taxonomic assignment of short sequence. No sequence suggestive of a new viral species was identified. Discussion This study did not identify any new blood-borne virus in high-risk individuals. However, rare and/or viruses present at very low titre could have escaped our protocol. Our results demonstrate the positive contribution of HTS in the detection of viral sequences in blood donations. PMID:27136432

  19. [Infectious markers among blood donors in Democratic Republic of Congo (DRC)].

    Science.gov (United States)

    Batina, A; Kabemba, S; Malengela, R

    2007-01-01

    In sub-saharian Africa, two factors account for the difficulties encountered to reach optimal blood safety: high frequency in the general population of various infections of which some are transmissible by blood transfusion and a still insufficient proportion of voluntary donors which constitute the safest group. The Kisangani transfusion center in DRC does not escape from this rule since in addition to voluntary blood donors (29.2%), its blood supply is mainly assured by family (or replacement) donors (69.2%). Persistence of a few remunerated donors (1.6%) was also noted at the period of the study. In this study, we determined seroprevalence of HIV, HBV and of syphilis infections in these three categories of donors and defined their characteristics by a retrospective analysis carried out on 3.390 subjects between January 2003 to December 2004. It revealed that 4.7% of the donors were positive for HIV, 5.4% for HBV and 3.7% for syphilis. There were significant differences according to studied groups : voluntary blood donors (n=989; HIV+ = 2.2%; VHB+ =3%; syphilis+ = 1.1%), family donors (n = 2.345; HIV+ = 4.6%; HBV+ = 4.9%; syphilis+ = 3.6%) and remunerated donors (n=56; HIV=50%; HBV+ = 64.3%; syphilis+ = 53.6%). These results indicate that it is necessary to intensify promotion of voluntary donation by a policy of information and education and to abolish practice of remunerated donation. Within the limits of possible, family donation should be gradually discouraged.

  20. Phenotypic Profile of Rh and Kell Blood Group Systems among Blood Donors in Cote d'Ivoire, West Africa

    Science.gov (United States)

    Siransy Bogui, L.; Dembele, B.; Sekongo, Y.; Abisse, S.; Konaté, S.; Sombo, M.

    2014-01-01

    Few countries in sub-Saharan Africa make systematic searches for antigens C, c, E, and e of the Rh and Kell system antigens in the donor and recipient, thereby exposing transfused patients. Purpose and Objectives. In this paper, we propose to determine the red cell Rh and Kell blood groups among blood donors from traditional techniques to improve medical care of transfused patients. This study will allow us to assess the frequency of blood group antigens in these systems. Study Design and Methods. We carried out a study on the red cell typing in the blood donor population of the National Blood Transfusion Center in Abidjan. This study was performed on 651 blood donors. Results. For the Rh system, the antigen frequencies of D, c, e, C, and E are, respectively, 92.93%, 99.85%, 99.85%, 21.97%, and 13.82%. K antigen is found in 0.77% of donors. Discussion and Conclusion. Although the frequencies of the most immunogenic antigens are lower than in the white race, lack of preventive measures makes the immunological risk high in Africa. Furthermore, Africa is full of specificities that are important to note for a better care of our patients. PMID:25328758

  1. Risk of cancer after blood transfusion from donors with subclinical cancer: a retrospective cohort study

    DEFF Research Database (Denmark)

    Edgren, Gustaf; Hjalgrim, Henrik; Reilly, Marie

    2007-01-01

    BACKGROUND: Although mechanisms for detection of short-term complications after blood transfusions are well developed, complications with delayed onset, notably transmission of chronic diseases such as cancer, have been difficult to assess. Our aim was to investigate the possible risk of cancer...... transmission from blood donors to recipients through blood transfusion. METHODS: We did a register-based retrospective cohort study of cancer incidence among patients who received blood from donors deemed to have a subclinical cancer at the time of donation. These precancerous donors were diagnosed...... with a cancer within 5 years of the donation. Data from all computerised blood bank registers in Sweden and Denmark gathered between 1968 and 2002 were merged into a common database. Demographic and medical data, including mortality and cancer incidence, were ascertained through linkages with nationwide...

  2. Relation between Helicobacter pylori infection, thyroid hormone levels and cardiovascular risk factors on blood donors.

    Science.gov (United States)

    Triantafillidis, John K; Georgakopoulos, Dimitrios; Gikas, Aristofanis; Merikas, Emmanuel; Peros, George; Sofroniadou, Kyriaki; Cheracakis, Petros; Sklavaina, Maria; Tzanidis, Georgios; Konstantellou, Evangelia

    2003-12-01

    The aim of this study was to assess the relationship between Helicobacter pylori (Hp) infection, serum thyroid hormone levels and certain cardiovascular risk factors in normal volunteers. In 110 blood donors (85 men, 25 women, aged 35.6 +/- 9.76) the serum levels of IgG antibodies against Hp were estimated using a sensitive immunoassay. Serum estimation of T3, T4, TSH, FT3, FT4, thyroid (microsomial) autoantibodies, C-Reactive-Protein, a1-acid-glycoprotein, vitamin B12, folic acid, cholesterol, triglycerides, total lipids, HDL, LDL, and antibodies against hepatitis A, was also carried-out. In all subjects a number of clinicoepidemiological parameters including body mass index, smoking habits, educational level, number of siblings and presence of symptoms from the digestive system were carefully recorded. Statistical analyses were performed using the SPSS statistical package. Helicobacter pylori infection was found in 54 subjects (49.1%). On univariate analysis, significant differences between subjects positive and negative for Helicobacter pylori infection were found for FreeT3 (3.11 +/- 0.5 pmol/ vs. 3.42 +/- 0.8 pmol/l, P=0.025), FreeT4 (1.04 +/- 0.2 ng/dl vs. 1.17 +/- 0.3 ng/dl, P=0.025), and thyroid autoanti bodies (23.65 +/- 24 vs. 14.97 +/- 8, P=0.018). Significant differences were also found for Cholesterol (207.8 +/- 39 mg/dl vs. 193.3 +/- 40 md/dl, P=0.05), LDL (133.2 +/- 32 mg/dl vs. 119.6 +/- 40 mg/dl, P=0.05) and folic acid (7.66 +/- 3.7 ng/ml vs. 6.39 +/- 2.5 ng/ml, P=0.038). A significantly positive correlation of Hp infection with age and number of siblings and a negative one with educational level were noticed. No differences concerning the levels of acute phase proteins, vitamin B12, antibodies against hepatitis A, body mass index, and smoking habits were found. On logistic regression analysis, significant differences remained only for thyroid autoantibodies (Odds ratio for titer ?30: 7.8, P=0.012), age (Odds Ratio for those aged >40 years vs those

  3. Investigating the Importance of Haemoglobin Measurement for Selection of Blood Donors in Libya

    Science.gov (United States)

    Abud, AI; Bashein, AM; Msalati, AA

    2009-01-01

    In Libya, haemoglobin estimation is not used routinely to assess the fitness of blood donors. We examined the importance of including this parameter in donor selection. Venous blood samples were collected from the bleed line at the end of the donation process from 1339 healthy adult male blood donors, aged 18–60 years, in Al-Jala Women Hospital in Tripoli, Libya. Haemoglobin concentration was measured using an automated haematology analyzer. The main donor age categories were 21–30 and 31–40 years (49.5% and 37%, respectively). Blood donation was mainly a replacement donation. The mean ±2SD range for haemoglobin was 10.8–17.0 g/dl. 27.2% of the donors had haemoglobin concentration of less than 13 g/dl, 16.9% of them had less than 12.5 g/dl and 1.9% had less than 10.8.5 g/dl. Our results suggest that it is important to include haemoglobin estimation as a routine procedure in selection of blood donors. PMID:21483535

  4. The evaluation of iron deficiency and anemia in male blood donors with other related factors

    Directory of Open Access Journals (Sweden)

    Yousefinejad Vahid

    2010-01-01

    Full Text Available Aims and Background: Iron deficiency is one of the most common nutritional disorders worldwide and blood donation may cause iron depletion. Limited studies with large sample size have been done on male donors. The aim of this study is to determine the prevalence of iron deficiency and iron deficiency anemia among male donors in the Kurdistan Organization of Blood Transfusion in Iran. Materials and Methods: This was a cross-sectional study. Sample size was 1184 blood donors selected by systematic random sampling. Hemoglobin, serum iron, serum ferritin, total iron banding capacity (TIBC and transferin saturation were measured in donors. Iron depletion, lack of iron stores, iron deficiency, iron deficiency anemia and anemia were evaluated among them. Data was analyzed with SPSS software and X΂, one-way ANOVA, and LSD test. Results: Iron deficiency, anemia, iron deficiency anemia, iron depletion and lack of iron resources were seen in 2.3, 4.08, 2.14, 22.76 and 4.66 percent respectively. There was a significant relationship of iron deficiency and iron deficiency anemia with instances of donation and interval from last donation (P < 0.05. A significant relationship was seen between iron deficiency and iron deficiency anemia among blood donors with more than ten times blood donation (P < 0.05. Conclusions: This study showed regular male donors require especial attention. Therefore, serum ferritin is recommended as a more adequate index to use for iron deficiency screening and planning purposes for iron supplementation among them.

  5. [Prevalence and incidence of HIV and hepatitis B among blood donors and estimated residual risk of transmission of HIV and HBV virus by blood transfusion. A study at the Provincial General Referee Hospital Bukavu, Democratic Republic of the Congo].

    Science.gov (United States)

    Namululi, B A; Guerrieri, C; Dramaix, M W

    2013-04-01

    To estimate the residual risk of transmission of HIV and HBV virus by blood transfusion in Bukavu. Retrospective cohort study designed for exploratory purposes, which took place in Bukavu (DR Congo) between January 2001 and December 2005, among 3292 blood donors. The incidences were estimated by survival curves and Cox models. The adjusted relative risks with their confidence interval at 95% were derived from Cox models. The residual risk of viral transmission associated with the serological window is equal to the incidence rate multiplied by the duration of the serological window divided by 365. The prevalence among blood donors in Bukavu was 1% for HIV and 3.7% for HbsAg. The number of incident cases observed was seven for HIV and 40 for hepatitis B between 2001 and 2005. The incidence rates obtained were 3.57 for 1000 person-years (0.93/1000-6.23/1000) and 25.4 per 1000 person-years (17.6/1000-33.36/1000), respectively for HIV and hepatitis B. The residual risk was 1/4608 donations for HIV or 0.22 (0.02-0.65) and 1/257 donations for HBV or 3.90 (1.20-9.96). Also there were more seroconversions among family blood donors than in volunteer donors. The risk of seroconversion in family donors compared to volunteer donors adjusted for age, sex and residence was 7.09 (3.75-13.39) for HIV and 4.03 (2.63-6.20) for HBsAg. The same result was observed with the survival curves. The prevalences of HIV and HBsAg in Bukavu are lower than in most major cities in sub-Saharan Africa. Residual risks are especially important for hepatitis B. Copyright © 2013 Elsevier Masson SAS. All rights reserved.

  6. Seroprevalence of transfusion transmissible infections among blood donors at the blood bank of a Medical College of Kolkata

    Directory of Open Access Journals (Sweden)

    Prasanta Ray Karmakar

    2014-01-01

    Full Text Available Seroprevalence of transfusion transmissible infections (TTIs among blood donors can be used to monitor the prevalence among apparently healthy adult population. The present study was conducted to determine the profile of blood donors and seroprevalence of TTI among them. Retrospective analysis of the donors of a blood bank attached with a tertiary care hospital of Kolkata in 2011 was carried out. Data were analyzed with SPSS version 17. Majority (85% of the donors were male, two-third in the age group of 21-40 years. Among the donors 2.79% were positive for any of the screened TTIs. Seroprevalence was highest for hepatitis B (1.41% followed by human immunodeficiency virus (0.60% and hepatitis C (0.59% and least for syphilis (0.23%. Seropositivity increased with age up to 50 years. There was no significant difference in seropositivity between male and female. Highly sensitive donor screening and public awareness program can make transfusion of blood products safe.

  7. Seroprevalence of transfusion transmissible infections among blood donors at the blood bank of a Medical College of Kolkata.

    Science.gov (United States)

    Karmakar, Prasanta Ray; Shrivastava, Prabha; Ray, Tapobrata Guha

    2014-01-01

    Seroprevalence of transfusion transmissible infections (TTIs) among blood donors can be used to monitor the prevalence among apparently healthy adult population. The present study was conducted to determine the profile of blood donors and seroprevalence of TTI among them. Retrospective analysis of the donors of a blood bank attached with a tertiary care hospital of Kolkata in 2011 was carried out. Data were analyzed with SPSS version 17. Majority (85%) of the donors were male, two-third in the age group of 21-40 years. Among the donors 2.79% were positive for any of the screened TTIs. Seroprevalence was highest for hepatitis B (1.41%) followed by human immunodeficiency virus (0.60%) and hepatitis C (0.59%) and least for syphilis (0.23%). Seropositivity increased with age up to 50 years. There was no significant difference in seropositivity between male and female. Highly sensitive donor screening and public awareness program can make transfusion of blood products safe.

  8. Sero - Prevalence of Viral Transfusion-transmissible Infections amongst voluntary Blood donors

    Directory of Open Access Journals (Sweden)

    Rashida Elrashid Mohamed Ali

    2016-02-01

    Full Text Available This study aimed to determine the Sero-prevalence of viral transfusion-transmissible Infectious diseases among blood donors, namely immunodeficiency virus, hepatitis B and C transmissible infections (TTIs like HBV, HCV. HIV (Human immune viruses.. sero-prevalence of viral transmissible infections. The donated blood for specific antibodies for infections agents. Can largely reduce the risk of TTIs, virus among blood donors. The study was carried out in the blood bank at Khartoum Teaching Hospital, centre, Sudan. Screening of blood samples for hepatitis B surface Antigen (HBsAg, Human immunodeficiency virus (HIV and hepatitis C virus (HCV Antibodies were done using (ELISA enzyme link immunoassay. The study included (1184 voluntary Blood donors, all were males. The overall prevalence of viral transfusion transmissible Infections were (11.84%. The sero-prevalence for antibody against HIV (6 and hepatitis C Virus was positive in 8 (0.06 and (0.08% donors respectively while HBsAg was detected in 98 (9.8% donors.  situation that need for strict criteria for selection of blood donors and also methods of laboratory assays. Services are high in Sudan due to the endemicity of infections like malaria, nutritional problem and obstetrical emergencies associated with blood loss. Little is known about the level of these infections in Sudan so; this study was conducted to investigate the sero-prevalence of transfusion transmissible viral infectious diseases in particular human B and hepatitis Immunodeficiency, hepatitis C viruses. The mode of transmission for HIV, HBV and HCV is the same and includes unsafe Sexual sharp materials Contact, using contaminated with body fluid, mother to Child and transfusion of blood and blood Products.

  9. Knowledge and awareness about Iron deficiency and megaloblastic anaemia among blood donors: a study at rural based tertiary care hospital

    Directory of Open Access Journals (Sweden)

    Amar R. Shah

    2015-06-01

    Full Text Available Background: Voluntary blood donation is promoted in order to make the blood banking safe and successful. Research in the area of blood donation has found that, iron stores are influenced by regular blood donation if dietary intake of iron is inadequate. Awareness and knowledge among blood donors regarding iron and B12 deficiency and its prevention is very much required. Objective: To assess the knowledge and awareness about iron deficiency and megaloblastic anaemia among blood donors. Methods: A cross sectional study was conducted among the 500 voluntary blood donors during the span of 1 year at one of the blood bank of tertiary care hospital using prestructured questionnaire on the various aspect of iron and B12 deficiency/folate deficiency anemia. The data was analysed with the help of Microsoft excel and SPSS. Results: Out of 500 blood donors, 15.6% donors were regular blood donor. It was observed 60% blood donors were having knowledge of anemia in general. Iron deficiency and vitamin B12 deficiency were known to 42% and 31.6% donors, respectively. Only 20% donors could able to answer the acceptable level of hemoglobin require for donating the blood. About 42% donors were aware about importance of iron, folate and vitamin B12 in maintaining normal hemoglobin level. Majority (82.7% of regular blood donors were willing to get information regarding iron, folate and vitamin B12 deficiency. Conclusion: Significant lack of awareness regarding iron and vitamin B12 deficiency was observed in regular voluntary blood donors. The present study recommends the provision of health education on iron and vitamin B12 deficiency as well as Iron, folate and vitamin B12 rich foods to regular blood donor to prevent anaemia among them. [Int J Res Med Sci 2015; 3(3.000: 708-710

  10. Comparison of Stored Umbilical Cord Blood and Adult Donor Blood: Transfusion Feasibility

    Directory of Open Access Journals (Sweden)

    Rola Sahyoun-tokan

    2012-09-01

    Full Text Available OBJECTIVE: This study aimed to compare the storage properties of red blood cell (RBC concentrates of umbilical cord blood (UCB and adult donor blood (ADB, and to evaluate the feasibility of UCB-RBC concentrate as an autologous source for blood transfusion in very low birth weight (VLBW preterm neonates. METHODS: In all, 30 newborn (10 preterm, 20 full term UCB and 31 ADB units were collected. RBC concentrates were stored and compared with regard to pH, potassium (K+, 2,3-biphosphoglycerate (2-3-BPG, adenosine tri-phosphate (ATP, plasma Hb, and bacterial contamination on d 1, 21, and 35 of storage. RESULTS: The K+ level increased with time and differed significantly between storage d 1 and 21, and between storage d 1 and 35 in both the UCB and ADB units. Initial and d 21 K+ levels were higher in the UCB units than in the ADB units. The 2,3-BPG level did not differ significantly between the UCB-PRC and ADB-PRC samples. After 35 d of storage both UCB-PRC and ADB-PRC samples exhibited significant differences from the initial free Hb, intracellular ATP, and pH values. Significant differences in intracellular ATP and pH were also observed between the UCB-PRC and ADB-PRC samples. CONCLUSION: The volume of harvested and prepared UCB-PRC can be used for some of the blood transfusions required during the neonatal period and thus may decrease the number of allogeneic transfusions, especially in preterm newborns. The hematological and biochemical changes that occurred in UCB during storage were comparable with those observed in ADB, and do not pose a risk to the immature metabolism of neonates. UCB-RPC prepared and stored under standard conditions can be a safe alternative RBC source for transfusions in VLBW newborns.

  11. Prevalence of HAV among Healthy Blood Donors Referring to Tehran Transfusion Center

    Directory of Open Access Journals (Sweden)

    A Elikaei

    2008-12-01

    Full Text Available "nBackground: Hepatitis A virus (HAV is a single strand and non-enveloped RNA virus. It is usually transmitted by the fe­cal-oral and the blood transfusion routes and causes the hepatitis A disease. Clinically, the hepatitis A is usually mild, par­ticularly in children, in whom it is frequently subclinical. The disease is more serious and prolonged in adults. The objective of this study was to find the prevalence of HAV among blood donors in Tehran, Iran."nMethods:  Sera of 407 blood donors in Tehran Blood Transfusion Center who were negative for anti-HIV, HBs Ag and anti-HCV were tested for total anti-HAV antibody, anti-HAV IgM and HAV RNA. Total antibodies (IgG+IgM and IgM were determined by ELISA using commercial kits. HAV RNA was detected by nested RT-PCR."nResults: The prevalence of total anti-HAV antibodies in blood donors were was 86%, and also the ratio 99% of blood do­nors was negative for anti-HAV IgM and 1% was equivocal. HAV RNA was not found in any serum samples. The preva­lence of total anti-HAV antibodies among blood donors was high; it means most of them were infected in childhood. In spite of, 14% of blood donors were negative for total anti-HAV antibodies; the prevalence of anti-HAV IgM and HAV RNA were very low."nConclusion:  Carefully blood donor selection is performed in Iran.

  12. Effect of blood bank storage on the rheological properties of male and female donor red blood cells.

    Science.gov (United States)

    Daly, Amanda; Raval, Jay S; Waters, Jonathan H; Yazer, Mark H; Kameneva, Marina V

    2014-01-01

    It was previously demonstrated that red blood cell (RBC) deformability progressively decreases during storage along with other changes in RBC mechanical properties. Recently, we reported that the magnitude of changes in RBC mechanical fragility associated with blood bank storage in a variety of additive solutions was strongly dependent on the donor gender [15]. Yet, the potential dependence of changes in the deformability and relaxation time of stored blood bank RBCs on donor gender is not known. The objective of this study was to determine the effects of donor gender and blood bank storage on RBC deformability and relaxation time through the measurement of RBC suspension viscoelasticity. Packed RBC units preserved in AS-5 solution from 12 male and 12 female donors (three from each ABO group) were obtained from the local blood center and tested at 1, 4 and 7 weeks of storage at 1-6°C. At each time point, samples were aseptically removed from RBC units and hematocrit was adjusted to 40% before assessment of cell suspension viscoelasticity. RBC suspensions from both genders demonstrated progressive increases (p blood bank storage may reduce tissue perfusion and RBC lifespan in patients receiving blood bank RBCs.

  13. Prevalence of hepatitis B virus infection among blood donors at the Tamale Teaching Hospital, Ghana (2009

    Directory of Open Access Journals (Sweden)

    Dongdem Julius

    2012-02-01

    Full Text Available Abstract Background Despite education and availability of drugs and vaccines, hepatitis B virus (HBV is still the most common severe liver infection in the world accounting for >1 million annual deaths worldwide. Transfusion of infected blood, unprotected sex and mother to child transmission are 3 key transmission routes of HBV in Ghana. There is high incidence of blood demanding health situations in northern Ghana resulting from anemia, accidents, malnutrition, etc. The higher the demand, the higher the possibility of transmitting HBV through infected blood. The aim of the investigation was to estimate the prevalence of HBV in blood donors which will provide justification for interventions that will help minimize or eliminate HBV infection in Ghana. Findings We investigated the prevalence of HBV infection among blood donors at Tamale Teaching Hospital. The Wondfo HBsAg test kit was used to determine the concentration of HBsAg in 6,462 (576 voluntary and 5,878 replacement donors as being ≥1 ng/ml. 10.79% of voluntary donors and 11.59% of replacement donors were HBsAg+. The 20-29 year group of voluntary donors was >2 times more likely to be HBsAg + than 40-60. Also the 20-29 year category of replacement donors was >4 times as likely to be HBsAg + than 50-69. Conclusions Risk of infection was age, sex and donor type dependent. The 20-29 year category had the highest prevalence of HBsAg + cases, mostly males residing within the metropolis.

  14. Blood donors at high risk of transmitting the acquired immune deficiency syndrome.

    Science.gov (United States)

    Contreras, M; Hewitt, P E; Barbara, J A; Mochnaty, P Z

    1985-03-09

    The acquired immune deficiency syndrome (AIDS) occurs most commonly in homosexual men. This group carries the greatest risk of transmitting AIDS by blood transfusion. Both promiscuous and nonpromiscuous male homosexuals should refrain from giving blood. A leaflet stating this advice was prepared by the Department of Health and Social Security, United Kingdom. In July 1984 a questionnaire was given to all donors attending a blood donor clinic in the west end of London, England. 53% were male. Donors were given a leaflet on AIDS and a questionnaire to complete in private. Those who considered themselves to be in a high risk group were asked to designate their blood for research purposes only. Serum samples from donors who confirmed that they were in the high risk category were tested for antihepatitis B core antigen and anti-human T lymphotropic virus type III (anti-HTLV-III) in addition to the routine screening of donors for hepatitis B surface antigen and syphilis. All high risk donors were men. Homosexuality was the only high risk factor. Of 5000 questionnaires administered between July and October, 614 were not completed or had ambiguous answers. 38 donors who completed the questionnaire beonged to a high risk group. Of these, 7 were positive for antihepatitis B core antigen; none were positive for anti-HTLV-III, T pallidum hemagglatination, or hepatits B surface antigen. Although the homosexual donors had a much lower incidence of sexually transmitted disease than those attending special clinics, this should not encourage complacency. All possible measures must be taken to prevent homosexuals from donating blood.

  15. Predictors of iron levels in 14,737 Danish blood donors

    DEFF Research Database (Denmark)

    Rigas, Andreas Stribolt; Sørensen, Cecilie Juul; Pedersen, Ole Birger

    2013-01-01

    BACKGROUND: Dietary studies show a relationship between the intake of iron enhancers and inhibitors and iron stores in the general population. However, the impact of dietary factors on the iron stores of blood donors, whose iron status is affected by blood donations, is incompletely understood...

  16. Protocol for a national blood transfusion data warehouse from donor to recipient.

    Science.gov (United States)

    van Hoeven, Loan R; Hooftman, Babette H; Janssen, Mart P; de Bruijne, Martine C; de Vooght, Karen M K; Kemper, Peter; Koopman, Maria M W

    2016-08-04

    Blood transfusion has health-related, economical and safety implications. In order to optimise the transfusion chain, comprehensive research data are needed. The Dutch Transfusion Data warehouse (DTD) project aims to establish a data warehouse where data from donors and transfusion recipients are linked. This paper describes the design of the data warehouse, challenges and illustrative applications. Quantitative data on blood donors (eg, age, blood group, antibodies) and products (type of product, processing, storage time) are obtained from the national blood bank. These are linked to data on the transfusion recipients (eg, transfusions administered, patient diagnosis, surgical procedures, laboratory parameters), which are extracted from hospital electronic health records. Expected scientific contributions are illustrated for 4 applications: determine risk factors, predict blood use, benchmark blood use and optimise process efficiency. For each application, examples of research questions are given and analyses planned. The DTD project aims to build a national, continuously updated transfusion data warehouse. These data have a wide range of applications, on the donor/production side, recipient studies on blood usage and benchmarking and donor-recipient studies, which ultimately can contribute to the efficiency and safety of blood transfusion. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

  17. Sero-prevalence of brucellosis among blood donors in Ahvaz, Southwest Iran

    Directory of Open Access Journals (Sweden)

    Abdolhussein Shakurnia

    2014-02-01

    Full Text Available Objective: To identify sero-prevalence of brucellosis among blood donors in Ahvaz city, Southwest Iran. Methods: A total number of 1 450 serum samples from blood donation were collected and were screened for the presence of brucella antibody. Rose Bengal Plate Test, Standard Agglutination Test (SAT, and 2-mercaptoethanol (2ME agglutination were tested in the sample. SAT dilution ≥1/80 and 2ME agglutination ≥1/40 were considered positive. Results: Sero-prevalence of brucellosis among the blood donors was 0.70%, 0.34%, and 0.20% by Rose Bengal Plate Test, SAT, and 2ME respectively. Conclusions: Considering the 1/80 titer of SAT as the criteria of contamination with brucella, routine screening of sero-prevalence of brucella in blood donors is not recommended in this area.

  18. Comparison of stored umbilical cord blood and adult donor blood: transfusion feasibility.

    Science.gov (United States)

    Tokan, Rola Sahyoun; Arsan, Saadet; Erdeve, Omer; Solaz, Nuri; Avcı, Aslıhan; Ulkar, Serenay Elgün; Gülyapar, Elif; Ustünyurt, Zeynep; Bıyıklı, Zeynep; Kemahlı, Sabri

    2012-09-01

    This study aimed to compare the storage properties of red blood cell (RBC) concentrates of umbilical cordblood (UCB) and adult donor blood (ADB), and to evaluate the feasibility of UCB-RBC concentrate as an autologoussource for blood transfusion in very low birth weight (VLBW) preterm neonates. In all, 30 newborn (10 preterm, 20 full term) UCB and 31 ADB units were collected.RBC concentrates were stored and compared with regard to pH, potassium (K(+)), 2,3-biphosphoglycerate (2-3-BPG),adenosine tri-phosphate (ATP), plasma Hb, and bacterial contamination on d 1, 21, and 35 of storage. The K(+) level increased with time and differed significantly between storage d 1 and 21, and between storaged 1 and 35 in both the UCB and ADB units. Initial and d 21 K(+) levels were higher in the UCB units than in the ADBunits. The 2,3-BPG level did not differ significantly between the UCB-PRC and ADB-PRC samples. After 35 d of storageboth UCB-PRC and ADB-PRC samples exhibited significant differences from the initial free Hb, intracellular ATP, andpH values. Significant differences in intracellular ATP and pH were also observed between the UCB-PRC and ADB-PRCsamples. The volume of harvested and prepared UCB-PRC can be used for some of the blood transfusions requiredduring the neonatal period and thus may decrease the number of allogeneic transfusions, especially in preterm newborns.The hematological and biochemical changes that occurred in UCB during storage were comparable with those observedin ADB, and do not pose a risk to the immature metabolism of neonates. UCB-RPC prepared and stored under standardconditions can be a safe alternative RBC source for transfusions in VLBW newborns.

  19. Risk factors for complications in donors at first and repeat whole blood donation: a cohort study with assessment of the impact on donor return.

    Science.gov (United States)

    Wiersum-Osselton, Johanna C; Marijt-van der Kreek, Tanneke; Brand, Anneke; Veldhuizen, Ingrid; van der Bom, Johanna G; de Kort, Wim

    2014-01-01

    First-time donation is among recognised risk factors for vasovagal reactions to blood donation and reactions are known to reduce donor return. We assessed associations between potential risk factors and vasovagal reactions and needle-related complications in first-time whole blood donation in comparison to repeat donation and analysed the impact of complications on donor return. We performed a cohort study on whole blood donations in The Netherlands from 1/1/2010 to 31/12/2010 using data extracted from the blood service information system. Donation data up to 31/12/2011 were used to ascertain donor return. In 2010 28,786 donors made first whole blood donations and there were 522,958 repeat donations. Vasovagal reactions occurred in 3.9% of first donations by males and 3.5% of first donations by females compared to in 0.2% and 0.6%, respectively, of repeat donations. Associations of vasovagal reactions with other factors including age, body weight, systolic and diastolic blood pressure were similar in first-time and repeat donors. Needle-related complications occurred in 0.2% of male and 0.5% of female first-time donations and in 0.1% and 0.3%, respectively, of repeat donations. Among first-time donors, the return rate within 1 year was 82% following an uncomplicated first donation, but 55% and 61% following vasovagal reactions and needle-related complications, respectively; the corresponding percentages among repeat donors were 86%, 58% and 82%. Among first-time donors, females suffered less than males from vasovagal reactions. Other risk factors had similar associations among first-time and repeat donors. Vasovagal reactions and needle-related complications in both first-time and repeat donors are followed by reduced donor return.

  20. Blood donor deferral: time for change? An evidence-based analysis

    Directory of Open Access Journals (Sweden)

    Borra V

    2016-05-01

    Full Text Available Vere Borra,1 Giovani Vandewalle,1 Hans Van Remoortel,1 Veerle Compernolle,1,2 Emmy De Buck,1 Philippe Vandekerckhove1–31Belgian Red Cross-Flanders, Mechelen, 2Faculty of Medicine, University of Ghent, Ghent, 3Department of Public Health and Primary Care, Faculty of Medicine, KU Leuven, Leuven, BelgiumAbstract: Donor selection remains an important part in the safety of the blood supply all over the world. Yet, donor deferral criteria seem to be strongly based on the precautionary principle protecting safety and quality, and on supply and expense considerations. This review therefore provides an overview of the available evidence on donor exclusion criteria, as well as on their cost-effectiveness, for the most frequent reasons of donor deferral in our region. PubMed was queried to retrieve primary research studies, systematic reviews, and health technology assessments (HTAs concerning donor exclusion criteria. With a similar approach, HTAs about the different blood-banking safety interventions were included. Reasons for donor deferral were recorded via the blood bank information system of the Belgian Red Cross-Flanders. Seven systematic reviews were identified: four on donor safety (hypotension, hypertension/type 2 diabetes, epilepsy, and higher age and three on recipient safety (hemochromatosis, men who have sex with men, and endoscopy. Forty-three low-quality observational studies were included, as well as 16 HTAs: three about donor exclusion criteria and 13 cost-utility analyses about blood-banking safety interventions. In general, the available evidence for deferral reasons was of low quality, and for 60% of the top 30 reasons for excluding donors, no evidence was found. Blood banking shows its unique position as many safety measures far exceed the normally accepted cost of €50,000/quality-adjusted life-years. The historical model based on the precautionary principle and on supply and expense considerations provides adequate supplies of

  1. Tattoos and transfusion-transmitted disease risk: implications for the screening of blood donors in Brazil

    Directory of Open Access Journals (Sweden)

    Sérgio de A. Nishioka

    2002-08-01

    Full Text Available Having a tattoo has been associated with serological evidence of hepatitis B and C viruses, as well as human immunodeficiency virus infections and syphilis; all of these are known to be transmissible by blood transfusion. These associations are of higher magnitude for individuals with nonprofessionally-applied tattoos and with two or more tattoos. Tattoos are common among drug addicts and prisoners, conditions that are also associated with transfusion-transmitted diseases. We examined the implications of these associations for the screening of blood donors in Brazil. Numbers of individuals who would be correctly or unnecessarily deferred from blood donation on the basis of the presence of tattoos, and on their number and type, were calculated for different prevalence situations based on published odds ratios. If having a tattoo was made a deferral criterion, cost savings (due to a reduced need for laboratory testing and subsequent follow-up would accrue at the expense of the deferral of appropriate donors. Restricting deferral to more `at-risk' sub-groups of tattooed individuals would correctly defer less individuals and would also reduce the numbers of potential donors unnecessarily deferred. Key factors in balancing cost savings and unnecessary deferrals include the magnitude of the pool of blood donors in the population, the prevalence of individuals with tattoos and the `culture' of tattoos in the population. Tattoos can therefore be an efficient criterion for the screening of blood donors in certain settings, a finding that requires corroboration from larger population-based studies.

  2. A SCREENING RESEARCH OF PLASMA BLOOD DONORS FOR MARKERS PARVOVIRUS INFECTION

    Directory of Open Access Journals (Sweden)

    Anastassia Ya. Antipova

    2015-01-01

    Full Text Available Parvovirus B19 (PV B19 replicates predominantly in progenitor cells of human erythrocytes and is transmitted by an airborne, vertical through and through blood or infected tissues. At-risk are pregnant women, people with immunodeficiency of different nature and individuals who need blood transfusions or organ transplantation. The available data indicate a high risk of infection through transfusion of blood containing the DNA of parvovirus B19, with viral load 105 copies/ml and above (Hourfar M.K. et al., 2011. According to the requirements of national regulations, the production of therapeutic drugs from plasma assumes the use of raw materials, free from viruses or with minimal viral load (Filatova E.C. et al., 2011. In some foreign countries a study of donor blood for the presence of DNA PV B19 is required; in our country the need for such screening is discussed (Giburt E.B. et al., 2013. Due to the fact that parvovirus is resistant to the methods of blood products desinfection, it is especially important to assess the quality of donor blood. Objective: To investigate the prevalence of the two markers parvovirus infection (IgG and PV B19 DNA in blood samples from one of the blood centers at St. Petersburg. Plasma samples from 100 blood donors from Military Medical Academy blood centre were tested by ELISA for the presence of IgG antibodies of parvovirus B19. Positive samples were tested by PCR for the DNA of parvovirus B19. ELISA test system recomWell Parvovirus B19 IgG (Microgen GmbH, Germany and diagnostic kits of Federal State Institution of Science «Central research Institute for epidemiology» of Rospotrebnadzor (Moscow, Russia which are approved for use in RF was used according to the manufacturers instructions. It was shown that 78 out of 100 donors aged 18 to 58 years had IgG-antibodies.76 positive blood plasma samples were investigated by PCR, with the 19 donors have found DNA of parvovirus B19 (25%. Viral load of one donor was 106

  3. Possibility of enhanced risk of retinal neovascularization in repeated blood donors: blood donation and retinal alteration

    Directory of Open Access Journals (Sweden)

    Rastmanesh R

    2011-09-01

    Full Text Available Reza RastmaneshDepartment of Clinical Nutrition and Dietetics, Shahid Beheshti University of Medical Sciences, National Nutrition and Food Technology Research Institute, Tehran, IranAbstract: Repeated blood donors manifest clinical, subclinical, and biochemical signs of iron deficiency anemia, have significantly higher erythropoietin and vascular endothelial growth factor (VEGF concentrations, and decreased tissue oxygen saturation, oxygenated tissue hemoglobin, and regional cerebral oxygen saturation. Erythropoietin and VEGF are potent retinal angiogenic factors which may initiate and promote the retinal angiogenesis process independently or simultaneously. Increases in circulating levels of erythropoietin and VEGF are proportionate to the levels of hematocrit, hypoxemia, and tissue hypoxia. It is suggested that higher erythropoietin production following iron deficiency anemia-induced chronic hypoxemia/hypoxia may, hypothetically, enhance the risk of retinal angiogenesis and/or neovascularization, possibly by inducing hypoxia inducible factor-1 alpha, which consequently upregulates genes stimulating angiogenesis, resulting in formation of a new vasculature, possibly by modulation of signal transducer and activator of transcription 3 signaling in the retina. Implications of this hypothesis cover erythropoietin doping, chronic hypoxia, and hypoxemic situations, such as angiogenesis-related cardiac and pulmonary diseases.Keywords: repeated blood donation, erythropoietin, retinal neovascularization, vascular endothelial growth factor, hypoxia

  4. Trends of transfusion transmissible diseases among blood donors at Uttarakhand, India

    Directory of Open Access Journals (Sweden)

    Gita Negi

    2014-01-01

    Full Text Available Context: Blood can save lives; however, it can be a source of transfusion transmitted diseases if proper screening of donated blood is not done. It is now mandatory to screen all donated blood units, whether replacement or voluntary for five transfusion transmitted diseases-namely human immunodeficiency virus (HIV, hepatitis B and C, syphilis, and malaria. Aims: The present study was done to study the prevalence of infectious disease markers among donors at the blood bank of a tertiary care center. Settings and Design: A total of 53,069 donors donated blood over 11 years. The number of replacement and voluntary donors was 41,710 and 11,359, respectively. Materials and Methods: Screening of blood units was done by enzyme-linked immunosorbent assay (ELISA method for HIV and hepatitis B and C. HIV testing was done using fourth generation ELISA kits. Syphilis was tested by latex agglutination assay and malaria was tested using slide method up to the year 2008-2009 and by rapid immunochromatographic assay after that. Results: The mean percentage of these infections per year was found to be 0.2, 1.2, 0.9, 0.3, and 0.002% for HIV, hepatitis B surface antigen (HBsAg, hepatitis C virus (HCV, syphilis, and malarial parasite (MP, respectively. Conclusions: The risk of transfusion transmissible infection (TTI today is low but supply of safe blood depends on proper donor selection and sensitive screening tests.

  5. Population-based screening for anemia using first-time blood donors

    Science.gov (United States)

    Mast, Alan E.; Steele, Whitney R.; Johnson, Bryce; Wright, David J.; Cable, Ritchard G.; Carey, Patricia; Gottschall, Jerome L.; Kiss, Joseph E.; Simon, Toby L.; Murphy, Edward L.

    2012-01-01

    Background Anemia is an important public health concern. Data from population-based surveys such as the National Health and Nutrition Examination Survey (NHANES) are the gold standard, but are obtained infrequently and include only small samples from certain minority groups. Objectives We assessed whether readily available databases of blood donor hemoglobin values could be used as a surrogate for population hemoglobin values from NHANES. Design Blood donor venous and fingerstick hemoglobin values were compared to 10,254 NHANES 2005-2008 venous hemoglobin values using demographically stratified analyses and ANOVA. Fingerstick hemoglobins or hematocrits were converted to venous hemoglobin estimates using regression analysis. Results Venous hemoglobin values from 1,609 first time donors correlated extremely well with NHANES data across different age, gender and demographic groups. Cigarette smoking increased hemoglobin by 0.26 to 0.59 g/dL depending on intensity. Converted fingerstick hemoglobin from 36,793 first time donors agreed well with NHANES hemoglobin (weighted mean hemoglobin of 15.53 g/dL for donors and 15.73 g/dL for NHANES) with similar variation in mean hemoglobin by age. However, compared to NHANES, the larger donor dataset showed reduced differences in mean hemoglobin between Blacks and other races/ethnicities. Conclusions Overall, first-time donor fingerstick hemoglobins approximate U.S. population data and represent a readily available public health resource for ongoing anemia surveillance. PMID:22460662

  6. Blood donor notification and counseling: Our experience from a tertiary care hospital in India

    Directory of Open Access Journals (Sweden)

    Urvershi Kotwal

    2015-01-01

    Full Text Available Aims: To evaluate the response rate of transfusion-transmissible infection (TTI-reactive donors after notification of their abnormal test results for the year 2012. Materials and Methods: This study is an observational descriptive study performed in our department over a period of 1 year. We evaluated the response rate of TTI-reactive donors after notification of their abnormal test results over 1 year as per the existing strategy (three telephonic and two postal communications. Results: During the study period, among the annual donation of 15,322 units, 464 blood donors were found to be seroreactive. Of these 464 seroreactive cases, 47 were HIV positive, 284 were reactive for Hepatitis B surface antigen (HBsAg, 49 were Hepatitis C (HCV positive and 84 were VDRL reactive. The TTI-reactive donors (464 for various markers were contacted: 229 (49.4% telephonically and the remaining 235 (50.6% not contacted on phone were informed by post. Of the 229 contacted donors, the response rate was 98.2% as only 225 donors reported (221 on the first, three on second and one on the third call for one to one counseling. The remaining four non-responders were - one HIV and three HBsAg reactive. The remaining 235 (50.6% reactive donors did not respond to any communication. Conclusion: Donor notification and post-donation counseling are an essential aspect of the blood bank that entails provision of information on serological status, assess the impact of test results on the donor and finally referral for medical care. As in our data only 49.4% of the blood donors could be contacted successfully, incomplete demographic details was the major limiting factor in communicating with rest. Of the 229 contacted donors, the response rate was 98.2%. A large majority (94.75% of the notified donors in our study contacted their health care provider when given clear instructions to do so. These results are encouraging because they indicate that a major element of the

  7. Structural Changes in the Surface of Red Blood Cell Membranes during Long-Term Donor Blood Storage

    Directory of Open Access Journals (Sweden)

    V. V. Moroz

    2012-01-01

    Full Text Available Objective: to study changes in the surface of red blood cell membranes of donor blood at the macro- and ultrastructural level during its storage for 30 days and to evaluate the functional state of the red blood cell membrane during the whole storage period. Material and methods. The investigation was conducted on human whole blood and packed red blood cells placed in the specialized packs containing the preservative CPDA-1, by using calibrated electroporation and atomic force microscopy and measuring plasma pH. Conclusion. The long-term, up to 30-day, storage of whole blood and packed red blood cells at 4°C was attended by lower plasma pH and increased hemolysis rate constant during calibrated electroporation and by the development of oxidative processes. The hemolysis rate constant was also higher in the packed red blood cells than that in the whole blood. On days 5—6, the membrane structure showed defects that developed, as the blood was stored, and caused irreversible cell membrane damage by day 30. Key words: donor blood, red blood cell membranes, atomic force microscopy.

  8. seroprevalence of parvovirus bi9 antibody in blood donors and ...

    African Journals Online (AJOL)

    boaz

    METHODS: This is a cross sectional study conducted at the Lagos University Teaching Hospital, Lagos Nigeria. .... eliminate competitive inhibition from specific IgG ... Males. Females. 80. 70. 144. 6. Positive history of Blood Transfusion.

  9. seroprevalence of hepatitis c virus antibodies amongst blood donors ...

    African Journals Online (AJOL)

    Dr Oboro VO

    SEROPREVALENCE OF HEPATITIS C VIRUS ANTIBODIES AMONGST BLOOD ... Conclusion: HCV infection is not uncommon in our environment hence the need to emphasize it's routine ... HCV antibody screening was done over a four-.

  10. Global Methylation and Hydroxymethylation in DNA from Blood and Saliva in Healthy Volunteers.

    Science.gov (United States)

    Godderis, Lode; Schouteden, Caroline; Tabish, Ali; Poels, Katrien; Hoet, Peter; Baccarelli, Andrea A; Van Landuyt, Kirsten

    2015-01-01

    Aims. We describe a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantify and compare simultaneously global methylation and hydroxymethylation in human DNA of different tissues. Materials and Methods. Blood and saliva DNA from fourteen volunteers was processed for epigenetic endpoints using LC-MS/MS and PCR-pyrosequencing technology. Results. Global DNA methylation was significantly lower in saliva (mean 4.61% ±  0.80%), compared to blood samples (5.70% ± 0.22%). In contrast, saliva (0.036% ± 0.011%) revealed significantly higher hydroxymethylation compared to blood samples (mean 0.027% ± 0.004%). Whereas we did not find significant correlations for both epigenetic measures between the tissues, a significant association was observed between global methylation and global hydroxymethylation in saliva DNA. Neither LINE-1 nor Alu elements of blood and saliva correlated, nor were they correlated with the DNA hydroxymethylation of blood or saliva, respectively. Conclusion. Global DNA methylation and hydroxymethylation of cytosine can be quantified simultaneously by LC-MS/MS. Saliva DNA cannot be considered as a surrogate for blood DNA to study epigenetic endpoints.

  11. Trends in Transfusion Transmitted Infections Among Replacement Blood Donors in Karachi, Pakistan

    Directory of Open Access Journals (Sweden)

    Syed Mohammad Irfan

    2013-06-01

    Full Text Available OBJECTIVE: To determine the prevalence of Hepatitis-B, Hepatitis-C and Human Immunodeficiency infections in replacement blood donors. METHODS: From January 2004 to December 2011, 108,598 apparently healthy donors donated blood at our Blood Bank. Screening was done by Microparticle Enzyme Immuno Assay (MEIA method on Axsym System (Abbott Diagnostic, USA and in year 2011 by Chemiluminescent Immunoassay (CIA method on Architect i2000 (Abbott Diagnostic, USA. From 2010 onward, HIV reactive donors were advised for confirmatory tests and reported back with the results. RESULTS: Of the 108,598 total donors, 108,393 (99.8% were replacement donors with a mean age of 28.92 (17-55 years. Of this, only 164 (0.15% were females. Among the replacement donors, 4,906 (4.5% were found to be reactive for Hepatitis-B, C and Human Immunodeficiency Virus. All the reactive patients, except one, were males. HbsAg was positive in 2,068 (1.90% and anti-HCV in 2832 (2.61% donors, while 111 (0.10% were positive for Human Immunodeficiency Virus. Co-infectivity was observed in 103 (0.09% cases. The prevalence appeared to be higher in younger age group (17-30 yrs. Only 16.6% cases should be patients returned with results of the confirmatory tests for HIV and were found positive. CONCLUSION: Hepatitis-B and C sero-prevalence in our series of replacement donors appears high compared to most studies from neighboring countries and relatively low in comparison to earlier studies from Pakistan. Prevalence of HIV, however, appears low and turn out of HIV positive cases for confirmatory tests is low.

  12. Techniques used for the screening of hemoglobin levels in blood donors: current insights and future directions

    Directory of Open Access Journals (Sweden)

    Chaudhary R

    2017-07-01

    Full Text Available Rajendra Chaudhary,1 Anju Dubey,2 Atul Sonker3 1Department of Transfusion Medicine, Sanjay Gandhi Postgraduate Institute of Medical Sciences, 2Department of Transfusion Medicine, T.S. Misra Medical College and Hospital, 3Department of Transfusion Medicine, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India Abstract: Blood donor hemoglobin (Hb estimation is an important donation test that is performed prior to blood donation. It serves the dual purpose of protecting the donors’ health against anemia and ensuring good quality of blood components, which has an implication on recipients’ health. Diverse cutoff criteria have been defined world over depending on population characteristics; however, no testing methodology and sample requirement have been specified for Hb screening. Besides the technique, there are several physiological and methodological factors that affect accuracy and reliability of Hb estimation. These include the anatomical source of blood sample, posture of the donor, timing of sample and several other biological factors. Qualitative copper sulfate gravimetric method has been the archaic time-tested method that is still used in resource-constrained settings. Portable hemoglobinometers are modern quantitative devices that have been further modified to reagent-free cuvettes. Furthermore, noninvasive spectrophotometry was introduced, mitigating pain to blood donor and eliminating risk of infection. Notwithstanding a tremendous evolution in terms of ease of operation, accuracy, mobility, rapidity and cost, a component of inherent variability persists, which may partly be attributed to pre-analytical variables. Hence, blood centers should pay due attention to validation of test methodology, competency of operating staff and regular proficiency testing of the outputs. In this article, we have reviewed various regulatory guidelines, described the variables that affect the measurements and compared the validated

  13. Cost and effectiveness comparison of two methods for screening potential blood donors for anaemia in Vietnam.

    Science.gov (United States)

    Tyrrell, A; Worrall, E; Que, T N; Bates, I

    2011-06-01

    To compare the cost and effectiveness of Copper Sulphate (CS) and HemoCue (HC) methods for screening blood donors for anaemia. Robust information from developing countries about cost and effectiveness of anaemia screening methods for blood donors is scarce. In such countries there are widespread shortages of blood, so the most cost-effective method should maximise blood supply without compromising donor safety. Economic data (e.g. staff time, equipment and buildings) were collected from direct observation of procedures and purchase data from Hanoi's Central Blood Bank administrative department. A framework for comparing the cost and effectiveness of anaemia screening methods was developed and a cost per effective (i.e. usable and accurate) test was generated for each method. Samples from 100 potential donors from the Hanoi Central Blood Bank (static) and 198 from two mobile units were tested. The mean probability of an ineffective anaemia test was 0·1 (0·05-0·2). The average cost of an HC test was $0·75 (static $0·61 and mobile $0·89) and a CS test was $0·31 (static $0·17 and mobile $0·45). The difference between static and mobile units was predominantly due to transport costs; the difference between the two methods was predominantly due to the HC microcuvettes. In this setting the CS yields greater value for money than the HC method for screening blood donors. The relative cost and effectiveness of CS and HC may be different in places with higher staff turnover, lower test accuracy, higher anaemia prevalence or lower workload than in Vietnam. © 2010 Liverpool School of Tropical Medicine. Transfusion Medicine © 2010 British Blood Transfusion Society.

  14. Changes in blood pressure among users of lay health worker or volunteer operated community-based blood pressure programs over time: a systematic review protocol.

    Science.gov (United States)

    Skar, Pål; Young, Lynne; Gordon, Carol

    2015-10-01

    reducing cardiovascular disease in specific hard-to-reach populations. Several systematic reviews have been conducted to assess different models for delivering services to people living with high blood pressure to assess community-based programs with a focus on cardiovascular disease, and to assess effectiveness of community health workers (CHW) in a variety of settings. These systematic reviews point to the importance of distinguishing between different categories of health care providers, their training and their roles in program delivery when assessing studies for possible inclusion in a systematic review.In a systematic review of studies from the US by Brownstein et al. focusing on the effectiveness of community health workers (CHWs) in the care of people with hypertension, this category of health care providers went under many different names. Community health workers in this review were defined as "any health workers who carried out functions related to health care deliver, were trained as part of an intervention, had no formal paraprofessional or professional designation, and had a relationship with the community being served". One of the findings from this review was the wide variety of formal training of the CHWs. In other parts of the world, a CHW might be defined differently. In their review of CHW-based programs focusing on children's health, Bhattacharyya, Winch, LeBan and Tien found that "in general CHWs are not paid salaries because the MOH (Ministry of Health) or donors do not consider salaries to be sustainable. Yet CHWs are often held accountable and supervised as if they were employees. Community health worker programs must recognize that CHWs are volunteers (emphasis in original), even if they receive small monetary or nonmonetary incentives. They are volunteering their time to serve the community". One Canadian model for delivering a cardiovascular awareness program designed to reach older adults through their primary care provider is based on

  15. Knowledge, attitude, and beliefs of young, college student blood donors about Human immunodeficiency virus

    Directory of Open Access Journals (Sweden)

    Anju Dubey

    2014-01-01

    Full Text Available Introduction: Young people, who tend to be healthy, idealistic, and motivated, are an excellent pool of potential voluntary unpaid blood donors. Recruiting and retaining young blood donors improves the long term safety and sufficiency of a country′s blood supply. Knowledge, attitude, and beliefs about Human immunodeficiency virus (HIV should play an important role in prevention of disease transmission. Materials and Methods: This study was a questionnaire based survey, conducted to explore the levels of knowledge, attitude, and beliefs about HIV in young college student blood donors. Results: The results showed that the proportion of participants with comprehensive knowledge of HIV prevention and transmission was lesser than expected. Increase in education level and male gender was found to be significantly associated with high HIV-related knowledge. The responses on the different aspects of HIV-related attitude were also varied and there is still stigma associated with Acquired Immunodeficiency Syndrome (AIDS even in the educated groups. Discussion: There was a spectrum of myths and misperceptions emphasizing the need of education that recognizes the social context of attitude towards HIV. Results from this study may contribute to the development of appropriate educational and training material for this group of donors which in turn, may assist in achieving the elusive goal of safe blood supply in future.

  16. Seroprevalence of Anti-Toxoplasma Gondii Antibodies in Healthy Voluntary Blood Donors from Mashhad City, Iran.

    Science.gov (United States)

    Zarean, Mehdi; Shafiei, Reza; Gholami, Maryam; Fata, Abdolmajid; Rahmati Balaghaleh, Mansour; Kariminik, Ashraf; Tehranian, Farahnaz; Hasani, Ali; Akhavan, Arash

    2017-07-01

    The present investigation aims to evaluate the prevalence of IgM and IgG anti-T. gondii antibodies and the associated risk factors among healthy blood donors in Mashhad city, Razavi Khorasan province, Iran. We screened a total of 500 serum samples by census method from apparently healthy blood donors of the Mashhad Blood Transfusion Organization (MBTO) for IgG and IgM anti-T. gondii antibodies by enzyme linked immunosorbent assay (ELISA). We found that 29.6%, 25%, 1.4%, and 3.2% of tested donors were positive for anti-T. gondii antibodies, only IgG antibody, both IgM and IgG, and IgM antibody alone, respectively. Several risk factors which were significantly related to T. gondii seropositivity in the univariate analysis at P sausage/hot dog consumption (OR = 4.472, P < 0.001). The results of this study can be a warning for blood transfusion organizations to pay special attention to toxoplasmosis among blood donors and also design screening programs for prevention of transfusion-transmitted toxoplasmosis.

  17. Effectiveness of DNA-recombinant anti-hepatitis B vaccines in blood donors: a cohort study

    Directory of Open Access Journals (Sweden)

    Petry Andrea

    2007-11-01

    Full Text Available Abstract Background Although various studies have demonstrated efficacy of DNA-recombinant anti-hepatitis B vaccines, their effectiveness in health care settings has not been researched adequately. This gap is particularly visible for blood donors, a group of significant importance in the reduction of transfusion-transmitted hepatitis B. Methods This is a double cohort study of 1411 repeat blood donors during the period 1998–2002, involving a vaccinated and an unvaccinated cohort, with matching of the two in terms of sex, age and residence. Average follow-up was 3.17 person-years. The outcome measure was infection with hepatitis B virus (HBV, defined by testing positive on serologic markers HBsAg or anti-HBC. All blood donors were from the blood bank in Joaçaba, federal state of Santa Catarina, Brazil. Results The cohorts did not differ significantly regarding sex, age and marital status but the vaccinated cohort had higher mean number of blood donations and higher proportion of those residing in the county capital Joaçaba. Hepatitis B incidences per 1000 person-years were zero among vaccinated and 2,33 among non-vaccinated, resulting in 100% vaccine effectiveness with 95% confidence interval from 30,1% to 100%. The number of vaccinated persons necessary to avoid one HBV infection in blood donors was estimated at 429 with 95% confidence interval from 217 to 21422. Conclusion The results showed very high effectiveness of DNA-recombinant anti-HBV vaccines in blood donors. Its considerable variation in this study is likely due to the limited follow-up and the influence of confounding factors normally balanced out in efficacy clinical trials.

  18. Dengue viremia in blood donors in Northern India: Challenges of emerging dengue outbreaks to blood transfusion safety

    Directory of Open Access Journals (Sweden)

    Sadhana Mangwana

    2015-01-01

    Full Text Available Backdround: Emerging infectious diseases pose threats to the general human population; including recipients of blood transfusions. Dengue is spreading rapidly to new areas and with increasing frequency of major outbreaks. Screening blood for dengue antigens in dengue-endemic countries would be costly and should, therefore, be recommended only after careful assessment of risk for infection and cost. Aim: A prospective study was conducted to establish the magnitude of the threat that dengue poses to blood safety where it is sporadic with seasonal variations, to quantify risk and to assess that whether screening is feasible and cost-effective. Materials and Methods: Nonstructural protein 1 (NS1 antigen test was done on 1709 donations during dengue outbreak in the months August to November 2013 as an additional test using Bio-Rad Platelia Dengue NS1AG test kit which is one step sandwich format microplate enzyme immunoassay using murine monoclonal antibodies for capture and revelation. Chi-square test was used to find statistical significance. Results and Conclusions: Majority cases were whole blood, replacement, male donors with 76.10% donors in <35 years age group. About 17.85% were single donor platelet donations. NS1 antigen in all donors was negative. In the past, dengue affected mainly children who do not donate blood. With the changing trend, mean age of infection increased affecting the population that does donate blood, further reducing blood donation pool. Further studies need to be done in different geographic regions of the country during dengue transmission season to establish maximum incidence of viremic donations, rates of transfusion transmission and clinical consequences in recipients. If risk is found to be substantial, decision will be taken by the policymakers at what threshold screening should be instituted to ensure safe blood transfusion.

  19. Distribution of Dengue Virus Types 1 and 4 in Blood Components from Infected Blood Donors from Puerto Rico.

    Science.gov (United States)

    Añez, Germán; Heisey, Daniel A R; Chancey, Caren; Fares, Rafaelle C G; Espina, Luz M; Souza, Kátia P R; Teixeira-Carvalho, Andréa; Krysztof, David E; Foster, Gregory A; Stramer, Susan L; Rios, Maria

    2016-02-01

    Dengue is a mosquito-borne viral disease caused by the four dengue viruses (DENV-1 to 4) that can also be transmitted by blood transfusion and organ transplantation. The distribution of DENV in the components of blood from infected donors is poorly understood. We used an in-house TaqMan qRT-PCR assay to test residual samples of plasma, cellular components of whole blood (CCWB), serum and clot specimens from the same collection from blood donors who were DENV-RNA-reactive in a parallel blood safety study. To assess whether DENV RNA detected by TaqMan was associated with infectious virus, DENV infectivity in available samples was determined by culture in mosquito cells. DENV RNA was detected by TaqMan in all tested blood components, albeit more consistently in the cellular components; 78.8% of CCWB, 73.3% of clots, 86.7% of sera and 41.8% of plasma samples. DENV-1 was detected in 48 plasma and 97 CCWB samples while DENV-4 was detected in 21 plasma and 31 CCWB samples. In mosquito cell cultures, 29/111 (26.1%) plasma and 32/97 (32.7%) CCWB samples were infectious. A subset of samples from 29 donors was separately analyzed to compare DENV viral loads in the available blood components. DENV viral loads did not differ significantly between components and ranged from 3-8 log10 PCR-detectable units/ml. DENV was present in all tested components from most donors, and viral RNA was not preferentially distributed in any of the tested components. Infectious DENV was also present in similar proportions in cultured plasma, clot and CCWB samples, indicating that these components may serve as a resource when sample sizes are limited. However, these results suggest that the sensitivity of the nucleic acid tests (NAT) for these viruses would not be improved by testing whole blood or components other than plasma.

  20. Surface antigen-negative hepatitis B virus infection in Dutch blood donors.

    Science.gov (United States)

    Lieshout-Krikke, R W; Molenaar-de Backer, M W A; van Swieten, P; Zaaijer, H L

    2014-01-01

    Hepatitis B virus (HBV) surface antigen (HBsAg) is a reliable marker for HBV infection, but HBsAg-negative forms of HBV infection occur. The introduction of HBV DNA screening of Dutch blood donors, which were not preselected for absence of HBV core antibodies, enabled the characterization of HBsAg-negative HBV infection in healthy persons and a comparison of the HBV genomes involved. The screening of 4.4 million Dutch blood donations identified 23 HBsAg-negative, HBV DNA-positive persons. Serological testing of the index donations, follow-up samples and archived earlier samples was performed to determine the nature of each HBV DNA-only case. Despite low viral loads HBV DNA could be sequenced in 14 out of 23 donors, allowing HBV genotyping and the analysis of mutations in the HBV surface gene. Four types of HBsAg-negative HBV infection were detected: infection in the early stage before occurrence of HBsAg; suppressed infection after vaccination; HBV genotype G infection with decreased HBsAg production; and chronic occult (HBsAg negative) HBV infection. In the donors with occult HBV genotype D infection the HBV surface gene showed multiple "escape" mutations in the HBsAg a-determinant and CTL epitopes, while in an occult genotype A case the surface gene showed no mutations. HBsAg-negative forms of HBV infection in healthy blood donors explain the ongoing transmission of HBV via blood transfusion, if donor screening is limited to HBsAg. The screening of blood donors for HBV DNA and HBV core antibodies seems to cover all stages and variants of HBV infection.

  1. ORIGINAL ARTICLE: Blood Donor’s Status of HIV, HBV, HCV and Syphilis in this Region of Marathwada, India

    Directory of Open Access Journals (Sweden)

    Rangrao H. Deshpande

    2012-07-01

    Full Text Available Aims & Objectives: Blood transfusion can cause the transmission of infections to recipients. This is an important mode of infection. The aim of study was to assess the prevalence of such type of infections among blood donors and to compare the seroprevalence of transfusion transmitted diseases in voluntary donors and replacement donors. Retrospective study of five years from Jan. 2007 to Dec. 2011 was done. This study was conducted at Blood bank, MIMSR Medical College Latur, Govt. Medical College, Latur and Bhalchandra Blood bank, Latur. Material & Methods: Total 10, 4925 donors were tested. Donors were screened for seroprevalence of HIV, HBC, HCV and Syphilis. Screening of HIV, HBV & HCV was done by ELISA method & Syphilis was screened by RPR type. Results: The comparison of seroprevalence of HIV, HBV, HCV & Syphilis in voluntary donors and replacement donors showed significant difference only for HIV in the years 2007, 2010, and 2011. Conclusion: The seroprevalence of transfusion transmitted diseases in the study is very low or negligible in voluntary donors as compared to replacement donors. There was a declining trend of seroprevalence for all the disease screened. But in our study the difference is not significant, which indicates that the selection of donors is of low quality. The selection of high quality voluntary donors should be achieved by creation of awareness by education of the prospective donor populations.

  2. ASSESSMENT OF SEROPREVALANCE OF HUMAN IMMUNODEFICIENCY VIRUS INFECTION AMONG BLOOD DONORS IN AND AROUND BELLARY, KARNATAKA STATE, INDIA

    Directory of Open Access Journals (Sweden)

    Huggi

    2014-11-01

    Full Text Available AIMS AND OBJECTIVES: The aim of the study was to analyse the seroprevalence of human immunodeficiency virus (HIV infection in the blood among healthy voluntary blood donors in and around Bellary. SAMPLE SIZE: 51,144 blood donors. STUDY DESIGN: Retrospective study. DURATION OF THE STUDY: Jan-2006 to Dec-2013. RESULTS: In the 8-year study period, 51,144 units of blood were collected. The Seroprevalence of HIV was found to be 0.38%. Also, the Seroprevalence of HIV in Voluntary Blood Donors and Replacement Blood Donors was found to be 0.35% and 0.81%. In males and female blood donors, the Seroprevalence was fond to be 0.38% and 0.39%. CONCLUSION: The 8 year study reveals that the Seroprevalence of HIV in replacement donors is nearly twice as that of voluntary donors and nearly equal in male and female donors. Screening the blood donors for IV infection has to be made mandatory and the tests should be of the highest quality. Education and awareness among people should be encouraged and imparted.

  3. Testing for HTLV 1 and HTLV 2 among blood donors in Western Saudi Arabia: prevalence and cost considerations.

    Science.gov (United States)

    Hindawi, S; Badawi, M; Fouda, F; Mallah, B; Mallah, B; Rajab, H; Madani, T A

    2017-06-28

    Screening all blood donors for human T-cell lymphotropic viruses 1 and 2 (HTLV 1 and HTLV 2) is mandatory in Saudi Arabia. The aim of this study is to evaluate the results and costs associated with the current testing policy for HTLV 1 and HTLV 2 in blood donors at King Abdulaziz University Hospital (KAUH), Jeddah. Donor-testing results from Blood Transfusion Services at KAUH were reviewed over a 10-year period, from January 2006 through December 2015. All donors were screened using chemiluminescent microparticle immunoassay. Reactive samples were then tested by Western blot for confirmation. Costs associated with testing were calculated. Data of 107 419 donations in the study period were reviewed. Saudi nationals constituted 51 168 donors (47·6%). Of 107 419 blood donors tested for HTLV 1 and HTLV 2 antibody, and 95 (0·088%) donors were reactive to screening tests. None of the samples found to be reactive to screening tests was positive by Western blot. The average cost of testing was US$ 171 870 per year. No donors were confirmed to have HTLV 1 and HTLV 2 in this cohort exceeding 100 000 donors. We propose changes to the policy mandating universal testing by replacing it with universal leukodepletion coupled with targeted screening to donors coming from endemic area or donors at risk. Such changes are expected to lead to a reduction of testing cost without affecting safety. © 2017 British Blood Transfusion Society.

  4. Mobilization and engraftment of peripheral blood stem cells in healthy related donors >55 years old.

    Science.gov (United States)

    Motlló, Cristina; Sancho, Juan-Manuel; Grífols, Joan-Ramon; Juncà, Jordi; Morgades, Mireia; Ester, Anna; Rodríguez, Inés; Vives, Susana; Batlle, Montserrat; Guardia, Ramon; Ferrà, Christelle; Gallardo, David; Millá, Fuensanta; Feliu, Evarist; Ribera, Josep-Maria

    2014-03-01

    The increasing scarcity of young related donors has led to the use of older donors for related allogeneic hematopoietic stem cell transplantation (HSCT). This study analyzed the influence of age on the results of mobilization of peripheral blood stem cells (PBSCs) in healthy donors as well as on the engraftment and outcome of HSCT. A retrospective analysis from a single center was performed comparing the results of PBSC mobilization from related healthy donors according to their age. The study included 133 consecutive related donors. The median age was 50 years (range, 4-77 years); 70 (53%) donors were males, and 44 (33%) were >55 years old. All donors were mobilized with granulocyte colony-stimulating factor for 5 days. The peak CD34(+) cell count in peripheral blood was higher in younger than in older donors (median, 90.5 CD34(+) cells/μL [range, 18-240 CD34(+) cells/μL] versus 72 CD34(+) cells/μL [range, 20-172.5 CD34(+) cells/μL], P = 0.008). The volume processed was lower in younger than in older donors (16,131 mL [range, 4424-36,906 mL] versus 18,653 mL [range, 10,003-26,261 mL], P = 0.002) with similar CD34(+) cells collected (579.3 × 10(6) cells [range, 135.14 × 10(6)-1557.24 × 10(6) cells] versus 513.69 × 10(6) cells [range, 149.81 × 10(6)-1290 × 10(6) cells], P = 0.844). There were no differences in time to recovery of neutrophils and platelets or in the incidences of acute and chronic graft-versus-host disease, overall survival, non-relapse mortality and relapse incidence. Donors >55 years old mobilized fewer CD34(+) cells and required a greater volume to collect a similar number of CD34(+) cells. The outcome of HSCT was not influenced by donor age. Donor age should not be a limitation for related allogeneic HSCT. Copyright © 2014 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.

  5. Hepatitis E in blood donors: investigation of the natural course of asymptomatic infection, Germany, 2011.

    Science.gov (United States)

    Vollmer, Tanja; Diekmann, Juergen; Eberhardt, Matthias; Knabbe, Cornelius; Dreier, Jens

    2016-09-01

    Asymptomatic hepatitis E virus (HEV) infections have been found in blood donors from various European countries, but the natural course is rarely specified. Here, we compared the progression of HEV viraemia, serostatus and liver-specific enzymes in 10 blood donors with clinically asymptomatic genotype 3 HEV infection, measuring HEV RNA concentrations, plasma concentrations of alanine/aspartate aminotransferase, glutamate dehydrogenase and bilirubin and anti-HEV IgA, IgM and IgG antibodies. RNA concentrations ranged from 77.2 to 2.19×10(5) IU/mL, with viraemia lasting from less than 10 to 52 days. Donors showed a typical progression of a recent HEV infection but differed in the first detection of anti-HEV IgA, IgM and IgG and seropositivity of the antibody classes. The diagnostic window between HEV RNA detection and first occurrence of anti-HEV antibodies ranged from eight to 48 days, depending on the serological assay used. The progression of laboratory parameters of asymptomatic HEV infection was largely comparable to the progression of symptomatic HEV infection, but only four of 10 donors showed elevated liver-specific parameters. Our results help elucidate the risk of transfusion-associated HEV infection and provide a basis for development of screening strategies. The diagnostic window illustrates that infectious blood donors can be efficiently identified only by RNA screening.

  6. Prevalence of hepatitis A viral RNA and antibodies among Chinese blood donors.

    Science.gov (United States)

    Sun, P; Su, N; Lin, F Z; Ma, L; Wang, H J; Rong, X; Dai, Y D; Li, J; Jian, Z W; Tang, L H; Xiao, W; Li, C Q

    2015-12-09

    Like other developing countries, China was reported to have a relatively high seroprevalence of anti-hepatitis A antibodies (anti-HAV). However, no studies have evaluated the prevalence of anti-HAV and HAV RNA among voluntary blood donors with or without elevated serum alanine transaminase (ALT) levels. Anti-HAV antibodies were detected using an enzyme-linked immunosorbent assay, and reverse transcription quantitative polymerase chain reaction was carried out for detection of HAV RNA. In the current study, we analyzed a total of 450 serum samples with elevated ALT levels (≥40 U/L) and 278 serum samples with non-elevated ALT levels. Seroprevalence rates of anti-HAV were 51.6% in donors with elevated ALT and 41.4% in donors with non-elevated ALT; however, none of the samples was positive for HAV RNA. The results of our study showed lower seroprevalence rates of anti-HAV in blood donors (irrespective of ALT levels) than those in published data on Chinese populations. Although donors with elevated ALT had statistically higher prevalence rates of anti- HAV than did those with non-elevated ALT, none of the serum samples had detectable levels of the active virus. In conclusion, our results demonstrate that the transmission of hepatitis A by blood transfusion will occur rarely.

  7. Burden of transfusion transmissible viral infections among blood donors at a tertiary care referral teaching hospital in South India

    Directory of Open Access Journals (Sweden)

    Suresh B

    2016-07-01

    Full Text Available Background: Blood serves as a vehicle for transmission of blood-borne pathogens including human immunodeficiency virus (HIV, hepatitis B virus (HBV and hepatitis C virus (HCV. The present study was conducted to estimate the prevalence of these transfusion transmitted infections (TTIs in blood donors. Methods: All blood donors presenting to the blood bank at our tertiary care teaching hospital were screened for HIV, HBV and HCV by using enzyme-linked immunosorbent assay (ELISA method. Results: During the period January to December 2014, 9958 blood donors were screened for viral markers. The prevalence of HIV, HBsAg and HCV was 0.36%, 1.67%, and 0.56% respectively. Conclusions: Although multiple critical steps are taken to minimize the risk of infection from transfusion of blood or blood products, this risk can never be entirely eliminated. Stringent donor selection, proper counseling and deferral/ self exclusion may reduce the seroreactivity in donated blood and wastage of resources.

  8. Minimal variation in anti-A and -B titers among healthy volunteers over time: Implications for the use of out-of-group blood components.

    Science.gov (United States)

    Sprogøe, Ulrik; Yazer, Mark H; Rasmussen, Mads Hvidkjær; Antonsen, Berit; Bistrup, Claus; Assing, Kristian

    2017-06-01

    Using potentially out-of-group blood components, like low titer A plasma and O whole blood, in the resuscitation of trauma patients is becoming increasingly popular. However, very little is known whether the donors' anti-A and/or anti-B titers change over time and whether repeated titer measurements on the same donor are required to ensure that each donation produces a low titer product. The anti-A and/or anti-B titers were measured on 56 healthy adult volunteers (47 blood donors; nine blood center personnel) every 3 months for 12 consecutive months using an automated solid phase analyzer. The results were expressed as log2 titer steps (e.g., titer 32 = 5 titer steps). Minor variations in the average anti-A and/or anti-B titers were seen over time; the maximum individual SD in each group was 1.50 (IgG anti-A) or 1.00 (IgM anti-A, IgM, and IgG anti-B). When the SDs for the four titer measurements from all 56 volunteers were combined as appropriate, the highest overall combined SD was 0.47 titer steps for IgG anti-A. This value corresponds to a 95% confidence interval for intraindividual variation in this antibody's titer over 12 months of 0.96 titer steps. Thus, based on one measurement, an IgG anti-A with a titer step of, for example, 6 would be expected to be in the range of titer step 5 to titer step 7 over the course of 1 year with 95% probability. The titers of anti-A and/or anti-B among healthy adults are stable over at least 1 year. This suggests that repeated titer measurements within a year on the same donor are not necessary if donations are made at 3 months or longer intervals. Diagnostic study, level V.

  9. Profile of blood donors and reasons for deferral in coastal South India

    Directory of Open Access Journals (Sweden)

    Unnikrishnan B

    2011-07-01

    Full Text Available BackgroundA blood transfusion is a life-saving procedure in manyinstances. An adequate supply of safe blood is ensured byexercising donor deferral criteria and screening forTransfusion Transmitted Infections (TTI. The aim of thispaper is to study the profile of blood donors and reasons fordonor deferral in coastal South India.MethodThe study was conducted at a tertiary care hospital inMangalore. All those who donated between 1 January 2008and 31 December 2008 were included in the study. Datawas collected using a pre-tested semi-structured proformaand analysed using SPSS version 11.5.ResultsMost of the donors were under the age of 25 (42.92%.Donors were predominantly male (95.20%. In terms ofoccupation, most subjects were students (28.01% followedby businessmen (18.61%. Slightly more than three-quartersof the donors (77.20% were replacement donors. The mainreasons for deferral were consumption of medication in thepast 72 hours (15.15%, hypertension (13.18%, a lowhaemoglobin level (12.34% and alcohol intake in the past72 hours (12.20%. Among the TTIs identified, most sampleswere positive for Hepatitis B surface Antigen – HBsAg(0.87% or tested positive for Anti-Hepatitis C (HCVantibodies (0.36%.ConclusionFrom the study it was concluded that the majority of thedonor population was young and educated. The reason fordonation was mainly replacement rather than voluntary.This issue needs to be addressed by exercising proactivemeasures to increase the number of voluntary, nonremunerated,low-risk donors.

  10. Donation frequency, iron loss, and risk of cancer among blood donors

    DEFF Research Database (Denmark)

    Edgren, Gustaf; Reilly, Marie; Hjalgrim, Henrik

    2008-01-01

    for cancers of the liver, lung, colon, stomach, and esophagus, which are thought to be promoted by iron overload (combined odds ratio [OR] = 0.70, 95% confidence interval [CI] = 0.58 to 0.84), but only among men and only with a latency of 3-7 years. The risk of non-Hodgkin lymphoma was higher among frequent......BACKGROUND: Long-term deleterious effects of repeated blood donations may be masked by the donors' healthy lifestyle. To investigate possible effects of blood donation and iron loss through blood donation on cancer incidence while minimizing "healthy donor effects," we made dose...... = 107140) were individually matched on sex, age, and county of residence. Using conditional logistic regression, we estimated relative risks of cancer according to number of blood donations made or estimated iron loss 3-12 years before a case patient was diagnosed with cancer. All statistical tests were...

  11. [Social marketing of blood donation: an analysis of new donors' predisposition].

    Science.gov (United States)

    Barboza, Stephanie Ingrid Souza; Costa, Francisco José da

    2014-07-01

    This article aims to understand the motivational factors that influence individuals to donate blood, in order to support the social marketing measures for recruiting new donors. Based on theoretical research, a structural model was developed to analyze influences and interactions of constructs in individuals' intentions to donate blood. A field survey used a structured questionnaire with a sample of 346 individuals. The analysis included descriptive analysis, evaluation of psychometric consistency, and structural equation modeling with partial least squares estimation. Fear showed a negative influence and the reference group a positive influence on individual predisposition to donate blood. This indicated that social marketing for blood donation can be more effective in encouraging new donors if it remains focused on these two factors.

  12. Defining and measuring blood donor altruism: a theoretical approach from biology, economics and psychology.

    Science.gov (United States)

    Evans, R; Ferguson, E

    2014-02-01

    While blood donation is traditionally described as a behaviour motivated by pure altruism, the assessment of altruism in the blood donation literature has not been theoretically informed. Drawing on theories of altruism from psychology, economics and evolutionary biology, it is argued that a theoretically derived psychometric assessment of altruism is needed. Such a measure is developed in this study that can be used to help inform both our understanding of the altruistic motives of blood donors and recruitment intervention strategies. A cross-sectional survey (N = 414), with a 1-month behavioural follow-up (time 2, N = 77), was designed to assess theoretically derived constructs from psychological, economic and evolutionary biological theories of altruism. Theory of planned behaviour (TPB) variables and co-operation were also assessed at time 1 and a measure of behavioural co-operation at time 2. Five theoretical dimensions (impure altruism, kinship, self-regarding motives, reluctant altruism and egalitarian warm glow) of altruism were identified through factor analyses. These five altruistic motives differentiated blood donors from non-donors (donors scored higher on impure altruism and reluctant altruism), showed incremental validity over TPB constructs to predict donor intention and predicted future co-operative behaviour. These findings show that altruism in the context of blood donation is multifaceted and complex and, does not reflect pure altruism. This has implication for recruitment campaigns that focus solely on pure altruism. © 2013 The Authors. Vox Sanguinis published by John Wiley & Sons Ltd. on behalf of International Society of Blood Transfusion.

  13. Prevention of post-transfusion hepatitis c transmission through donor blood and its components

    Directory of Open Access Journals (Sweden)

    A. V. Chechetkin

    2015-01-01

    Full Text Available The aim of organizational aspects of preventing the transmission of hepatitis C virus with donor blood and its components.Materials and methods. An activity of the blood service establishments in Russia for the prevention of HCV infection through transfusion of blood and its components on the basis of the analysis of sectoral statistical surveys was studied.Results. The frequency of detection of antibodies to hepatitis C virus in blood donors and its components during 2009–2013 decreased by more than 1,5 times. The percentage of donors who have identified markers of hepatitis C virus was significantly different in different regions: from 0,51% to 1,36%. The activity of the blood service implemented method of plasma quarantine resulting annually rejected from 0,32% to 0,23% as a result of the identified markers of HCV. Pathogen inactivated plasma volume increased in 3 times, the platelet concentrate in 3,2 times.Conclusion. To ensure the safety of donated blood and its components in the blood service effectively the modern technology use for to prevention transmission of the HCV: quarantine of plasma, donor selection and development, inactivation of pathogens. The degree of implementation in practice of nonpaid voluntary blood transfusions significantly increased and is characterized by regional features in recent years .

  14. Protocol for a national blood transfusion data warehouse from donor to recipient

    NARCIS (Netherlands)

    van Hoeven, Loan R; Hooftman, Babette H; Janssen, Mart P; de Bruijne, Martine C; de Vooght, Karen M K; Kemper, Peter; Koopman, Maria M W

    2016-01-01

    INTRODUCTION: Blood transfusion has health-related, economical and safety implications. In order to optimise the transfusion chain, comprehensive research data are needed. The Dutch Transfusion Data warehouse (DTD) project aims to establish a data warehouse where data from donors and transfusion

  15. 76 FR 51041 - Hemoglobin Standards and Maintaining Adequate Iron Stores in Blood Donors; Public Workshop

    Science.gov (United States)

    2011-08-17

    ... HUMAN SERVICES Food and Drug Administration Hemoglobin Standards and Maintaining Adequate Iron Stores in... workshop. The Food and Drug Administration (FDA) is announcing a public workshop entitled: ``Hemoglobin... discuss blood donor hemoglobin and hematocrit qualification standards in the United States, its impact...

  16. A genetic risk factor for low serum ferritin levels in Danish blood donors

    DEFF Research Database (Denmark)

    Sørensen, Erik; Grau, Katrine; Berg, Trine;

    2012-01-01

    /or restless leg syndrome (RLS) were investigated in two groups of female blood donors. The first group had low iron stores (serum ferritin ≤ 12 µg/L, n = 657), and the second group had normal to high iron stores (serum ferritin > 30 µg/L, n = 645). Genotype distribution for each of the SNPs was compared...

  17. Epidemiology of high-level parvovirus B19 viraemia among Dutch blood donors, 2003-2009

    NARCIS (Netherlands)

    K. Kooistra; H.J. Mesman; M. de Waal; M.H.G.M. Koppelman; H.L. Zaaijer

    2011-01-01

    Background and Objectives Plasma derivatives and blood components with low levels of parvovirus B19 (B19) seem not infectious, but recently infected, highly viraemic donors may transmit B19. We studied the incidence of high-level B19 viraemia (B19 DNA > 106 IU/ml) in 6 center dot 5 million Dutch blo

  18. Dengue Virus Transmission by Blood Stem Cell Donor after Travel to Sri Lanka; Germany, 2013

    Centers for Disease Control (CDC) Podcasts

    2014-09-22

    Dr. Mike Miller reads an abridged version of the article, Dengue Virus Transmission by Blood Stem Cell Donor after Travel to Sri Lanka; Germany, 2013.  Created: 9/22/2014 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 10/8/2014.

  19. Glucose-6-Phosphate Dehydrogenase Deficiency among Male Blood Donors in Sana’a City, Yemen

    Science.gov (United States)

    Al-Nood, Hafiz A.; Bazara, Fakiha A.; Al-Absi, Rashad; Habori, Molham AL

    2012-01-01

    Objectives To determine the prevalence of Glucose-6-phosphate dehydrogenase (G-6-PD) deficiency among Yemeni people from different regions of the country living in the capital city, Sana’a, giving an indication of its overall prevalence in Yemen. Methods A cross-sectional study was conducted among Yemeni male blood donors attending the Department of Blood Bank at the National Centre of the Public Health Laboratories in the capital city, Sana’a, Yemen. Fluorescent spot method was used for screening, spectrophotometeric estimation of G-6-PD activity and separation by electrophoresis was done to determine the G-6-PD phenotype. Results Of the total 508 male blood donors recruited into the study, 36 were G-6-PD deficient, giving a likely G-6-PD deficiency prevalence of 7.1%. None of these deficient donors had history of anemia or jaundice. Thirty-five of these deficient cases (97.2%) showed severe G-6-PD deficiency class II (<10% of normal activity), and their phenotyping presumptively revealed a G-6-PD-Mediterranean variant. Conclusion The results showed a significant presence of G-6-PD deficiency with predominance of a severe G-6-PD deficiency type in these blood donors in Sana’a City, which could represent an important health problem through occurrence of hemolytic anemia under oxidative stress. A larger sample size is needed to determine the overall prevalence of G-6-PD deficiency, and should be extended to include DNA analysis to identify its variants in Yemen. PMID:22359725

  20. Protocol for a national blood transfusion data warehouse from donor to recipient

    NARCIS (Netherlands)

    van Hoeven, Loan R; Hooftman, Babette H; Janssen, Mart P; de Bruijne, Martine C; de Vooght, Karen M K; Kemper, Peter; Koopman, Maria M W

    2016-01-01

    INTRODUCTION: Blood transfusion has health-related, economical and safety implications. In order to optimise the transfusion chain, comprehensive research data are needed. The Dutch Transfusion Data warehouse (DTD) project aims to establish a data warehouse where data from donors and transfusion rec

  1. Sero-prevalence of hepatitis C virus among blood donors in Lagos ...

    African Journals Online (AJOL)

    Sero-prevalence of hepatitis C virus among blood donors in Lagos, Nigeria. ... PROMOTING ACCESS TO AFRICAN RESEARCH ... The prevalence of Hepatitis C virus (HCV), one of the causative agents of viral hepatitis was investigated. ... Senegal (6); Sierra Leone (1); South Africa (96); South Sudan (1); Sudan (3) ...

  2. Hepatitis E virus seroepidemiology: a post-earthquake study among blood donors in Nepal.

    Science.gov (United States)

    Shrestha, Ashish C; Flower, Robert L P; Seed, Clive R; Rajkarnikar, Manita; Shrestha, Shrawan K; Thapa, Uru; Hoad, Veronica C; Faddy, Helen M

    2016-11-25

    As one of the causative agents of viral hepatitis, hepatitis E virus (HEV) has gained public health attention globally. HEV epidemics occur in developing countries, associated with faecal contamination of water and poor sanitation. In industrialised nations, HEV infections are associated with travel to countries endemic for HEV, however, autochthonous infections, mainly through zoonotic transmission, are increasingly being reported. HEV can also be transmitted by blood transfusion. Nepal has experienced a number of HEV outbreaks, and recent earthquakes resulted in predictions raising the risk of an HEV outbreak to very high. This study aimed to measure HEV exposure in Nepalese blood donors after large earthquakes. Samples (n = 1,845) were collected from blood donors from Kathmandu, Chitwan, Bhaktapur and Kavre. Demographic details, including age and sex along with possible risk factors associated with HEV exposure were collected via a study-specific questionnaire. Samples were tested for HEV IgM, IgG and antigen. The proportion of donors positive for HEV IgM or IgG was calculated overall, and for each of the variables studied. Chi square and regression analyses were performed to identify factors associated with HEV exposure. Of the donors residing in earthquake affected regions (Kathmandu, Bhaktapur and Kavre), 3.2% (54/1,686; 95% CI 2.7-4.0%) were HEV IgM positive and two donors were positive for HEV antigen. Overall, 41.9% (773/1,845; 95% CI 39.7-44.2%) of donors were HEV IgG positive, with regional variation observed. Higher HEV IgG and IgM prevalence was observed in donors who reported eating pork, likely an indicator of zoonotic transmission. Previous exposure to HEV in Nepalese blood donors is relatively high. Detection of recent markers of HEV infection in healthy donors suggests recent asymptomatic HEV infection and therefore transfusion-transmission in vulnerable patients is a risk in Nepal. Surprisingly, this study did not provide evidence of a large

  3. Frequencies of DEA blood types in a purebred canine blood donor population in Porto Alegre, RS, Brazil

    Directory of Open Access Journals (Sweden)

    Vanessa Sinnott Esteves

    2011-02-01

    Full Text Available The study of canine immunohematology is very important for veterinary transfusion medicine. The objective of this study was to determine the DEA blood type frequencies in a purebred canine blood donor population from Porto Alegre, RS, Brazil. One hundred clinically healthy purebred dogs were chosen, 20 dogs from each breed (Great Dane, Rottweiler, Golden Retriever, German Shepherd and Argentine Dogo. Blood samples were taken in ACD-A tubes and the MSU hemagglutination tube test (MI, USA was used to determine the blood types. The studied population presented general frequencies of 61% for DEA 1.1, 22% for DEA 1.2, 7% for DEA 3, 100% for DEA 4, 9% for DEA 5 and 16% for DEA 7. A significant association was found between breeds and certain combinations of blood types in this population. The results are in agreement with the literature since most part of the canine population studied was positive for DEA 1.1, the most antigenic blood type in dogs. Differences were found among the studied breeds and those should be considered when selecting a blood donor. The knowledge of blood types frequencies and their combinations in different canine populations, including different breeds, is important because it shows the particularities of each group, helps to keep a data bank of local frequencies and minimizes the risks of transfusion reactions.

  4. Prevalence of Principal Rh Blood Group Antigens in Blood Donors at the Blood Bank of a Tertiary Care Hospital in Southern India.

    Science.gov (United States)

    Gundrajukuppam, Deepthi Krishna; Vijaya, Sreedhar Babu Kinnera; Rajendran, Arun; Sarella, Jothibai Dorairaj

    2016-05-01

    Rhesus (Rh) antigen was discovered in 1940 by Karl Landsteiner and Wiener. Due to its immunogenicity along with A, B antigens, Rh D antigen testing was made mandatory in pre-transfusion testing. Presently there are more than 50 antigens in Rh blood group system but major ones are D, C, E, c, and e. Very few reports are available regarding their prevalence in India and no reports are available from Andhra Pradesh. To study the prevalence of principal Rh blood group antigens like D, C, E, c & e in the voluntary blood donors attending our blood bank. A prospective cross-sectional non interventional study was carried out on 1000 healthy blood donors from August 2013 to July 2014 at our blood bank. Donors were grouped and typed for ABO and Rh major antigens using monoclonal blood grouping reagents as per the manufacturer's instructions. Statistical analysis was carried out using SPSS version 16. Comparison of categorical data between antigen positive and negative individuals was done using Chi-square test. Descriptive statistics for the categorical variables were performed by computing the frequencies (percentages) in each category. Incidence was given in proportion with 95% confidence interval. A total of 1000 blood samples from donors were phenotyped. Among Rh antigens, e was the most common antigen (98.4%), followed by D-94.1%, C-88%, c-54.9% and E-18.8% with DCe/DCe (R1R1) (43.4%) being the most common phenotype and the least common phenotype is r'r' (0.1%). Database for antigen frequency to at least Rh blood group system in local donors helps to provide antigen negative blood to patients with multiple alloantibodies, minimize alloimmunization rate, and thereby improve blood safety.

  5. Reduction of exposure to blood donors in preterm infants submitted to red blood cell transfusions using pediatric satellite packs

    Directory of Open Access Journals (Sweden)

    Cristina Lika Uezima

    2013-09-01

    Full Text Available OBJECTIVE: In preterm newborn infants transfused with erythrocytes stored up to 28 days, to compare the reduction of blood donor exposure in two groups of infants classified according to birth weight. METHODS: A prospective study was conducted with preterm infants with birth weight <1000g (Group 1 and 1000-1499g (Group 2, born between April, 2008 and December, 2009. Neonates submitted to exchange transfusions, emergency erythrocyte transfusion, or those who died in the first 24 hours of life were excluded. Transfusions were indicated according to the local guideline using pediatric transfusion satellite bags. Demographic and clinical data, besides number of transfusions and donors were assessed. . Logistic regression analysis was performed to determine factors associated with multiple transfusions. RESULTS: 30 and 48 neonates were included in Groups 1 and 2, respectively. The percentage of newborns with more than one erythrocyte transfusion (90 versus 11%, the median number of transfusions (3 versus 1 and the median of blood donors (2 versus 1 were higher in Group 1 (p<0.001, compared to Group 2. Among those with multiple transfusions, 14 (82% and one (50% presented 50% reduction in the number of blood donors, respectively in Groups 1 and 2. Factors associated with multiple transfusions were: birth weight <1000g (OR 11.91; 95%CI 2.14-66.27 and presence of arterial umbilical catheter (OR 8.59; 95%CI 1.94-38.13, adjusted for confounders. CONCLUSIONS: The efficacy of pediatrics satellites bags on blood donor reduction was higher in preterm infants with birth weight <1000g.

  6. Low Prevalence of Brucella Agglutinins in Blood Donors in Central Province of Iran

    Directory of Open Access Journals (Sweden)

    Masoomeh Sofian

    2013-03-01

    Full Text Available Background: Brucellosis is a zoonotic disease of worldwide distribution and has great economic importance. Despite its control in many countries, it remains endemic in Iran. Brucellosis was investigated in many high risk occupational groups; however, few studies on the prevalence of brucellosis among blood donors are available. To determine the seroprevalence of brucellosis antibodies in blood donors, a serological study was carried out in central province of Iran.Materials and Methods: A total of 897 healthy blood donors with mean age 37.23 ± 10.9 years were enrolled in the study. Laboratory tests including Standard Tube Agglutination Test (STA and 2-mercaptoethanol (2ME agglutination were checked in all samples. STA dilution ≥ 1:80, and in the presence of 2-mercaptoethanol (2ME agglutination ≥ 20 was considered positive.Results: Out of 897 cases, 11.9% were inhabitants of rural areas. 41.5% had history of consumption of unpasteurized dairy products and 9.3% had history of contact with domestic animals. A very low level of Brucella agglutinins was present in 3(0.33% of the samples and only one sample (0.11% was found to be truly positive for Brucella agglutinins. 2ME was negative in all samples. None of these 4 subjects showed signs and symptoms of brucellosis in 6 months follow-up.Conclusion: On the basis of our data, brucellosis has no epidemiological and clinical importance in our blood donors; therefore, it is not recommended to perform screening tests such as, STA and 2ME to identify brucellosis antibodies in the sera of blood donors.

  7. Contribution of the Retrovirus Epidemiology Donor Study (REDS to research on blood transfusion safety in Brazil

    Directory of Open Access Journals (Sweden)

    Paula Loureiro

    2014-04-01

    Full Text Available The Retrovirus Epidemiology Donor Study (REDS program was established in the United States in 1989 with the purpose of increasing blood transfusion safety in the context of the HIV/AIDS and human T-lymphotropic virus epidemics. REDS and its successor, REDS-II were at first conducted in the US, then expanded in 2006 to include international partnerships with Brazil and China. In 2011, a third wave of REDS renamed the Recipient Epidemiology and Donor Evaluation Study-III (REDS-III was launched. This seven-year research program focuses on both blood banking and transfusion medicine research in the United States of America, Brazil, China, and South Africa. The main goal of the international programs is to reduce and prevent the transmission of HIV/AIDS and other known and emerging infectious agents through transfusion, and to address research questions aimed at understanding global issues related to the availability of safe blood. This article describes the contribution of REDS-II to transfusion safety in Brazil. Articles published from 2010 to 2013 are summarized, including database analyses to characterize blood donors, deferral rates, and prevalence, incidence and residual risk of the main blood-borne infections. Specific studies were developed to understand donor motivation, the impact of the deferral questions, risk factors and molecular surveillance among HIV-positive donors, and the natural history of Chagas disease. The purpose of this review is to disseminate the acquired knowledge and briefly summarize the findings of the REDS-II studies conducted in Brazil as well as to introduce the scope of the REDS-III program that is now in progress and will continue through 2018.

  8. Action plan to regain unnecessary deferred blood donors due to malaria risk in Turkey.

    Science.gov (United States)

    Değirmenci, Aysu; Döşkaya, Mert; Caner, Ayşe; Nergis, Sebnem; Gül, Kadri; Aydınok, Yeşim; Ertop, Tufan; Aksoy, Nurten; Korkmaz, Metin; Alkan, Mehmet Ziya; Üner, Ahmet; Gürüz, Yüksel

    2012-06-01

    Malaria was expected to be a major problem during blood donation in Turkey due to existence of malaria cases in southeastern region of Turkey. The present study aimed for the first time, to investigate malaria in "donors deferred for malaria risk" and to determine the regional rates of malaria deferral in Turkey. Blood samples were collected from several Blood Banks of southeastern provinces where local malaria cases still exist and from Blood Bank of Ege University Medical School (EUMS) located in western Turkey where malaria is eradicated decades ago. Plasmodium spp. and specific antibodies were investigated by stained smears, antigen detection, PCR and ELISA. Among the donors deferred for malaria risk, Plasmodium spp. were not detected by microscopy, PCR or antigen detection. Seroprevalances were 2% and 3.92% in western and southeastern regions, respectively. Rate of donor deferral for malaria risk was 0.9% in EUMS and deferrals were exclusively because of travel to southeastern Turkey. In southeastern provinces, deferrals were mainly due to malaria like fever history. The present study first time assessed regional rates of donor deferral due to malaria risk in Turkey. Previously, malaria was expected to be a major problem during blood donation in Turkey due to existence of malaria cases in southeastern region of Turkey. The results of the study showed that 97% of the deferrals were unnecessary. In conclusion, to reduce unnecessary donor deferrals in Turkey, in addition to comprehensive questioning for malaria history, the usage of a malaria antibody screening method should be initiated prior to deferral decision.

  9. External validation and updating of a Dutch prediction model for low hemoglobin deferral in Irish whole blood donors

    NARCIS (Netherlands)

    Baart, A.M.; Atsma, F.; McSweeney, E.N.; Moons, K.G.; Vergouwe, Y.; Kort, W.L. de

    2014-01-01

    BACKGROUND: Recently, sex-specific prediction models for low hemoglobin (Hb) deferral have been developed in Dutch whole blood donors. In the present study, we validated and updated the models in a cohort of Irish whole blood donors. STUDY DESIGN AND METHODS: Prospectively collected data from 45,031

  10. HIV-1/2 indeterminate Western blot results: follow-up of asymptomatic blood donors in Belo Horizonte, Minas Gerais, Brazil

    Directory of Open Access Journals (Sweden)

    CARNEIRO-PROIETTI A.B.F.

    1999-01-01

    Full Text Available The clinical and public health importance of indeterminate results in HIV-1/2 testing is still difficult to evaluate in volunteer blood donors. At Fundação Hemominas, HIV-1/2 ELISA is used as the screening test and, if reactive, is followed by Western blot (WB. We have evaluated 84 blood donors who had repeatedly reactive ELISA tests for HIV-1/2, but indeterminate WB results. Sixteen of the 84 donors (19.0% had history of sexually transmitted diseases; 18/84 (21.4% informed receiving or paying for sex; 3/84 (3.6% had homosexual contact; 2/26 women (7.6% had past history of multiple illegal abortions and 3/84 (3.6% had been previously transfused. Four out of 62 donors (6.5% had positive anti-nuclear factor (Hep2, with titles up to 1:640. Parasitological examination of the stool revealed eggs of S. mansoni in 4/62 (6.4% donors and other parasites in 8/62 (12.9%. Five (5.9% of the subjects presented overt seroconversion for HIV-1/2, 43/84 (51.2% had negative results on the last visit, while 36/84 (42.9% remained WB indeterminate. Although some conditions could be found associated with the HIV-1/2 indeterminate WB results and many donors had past of risky behavior, the significance of the majority of the results remains to be determined.

  11. Significance of isolated hepatitis B core antibody in blood donors from São Paulo

    Directory of Open Access Journals (Sweden)

    ALMEIDA NETO Cesar de

    2001-01-01

    Full Text Available The clinical significance of isolated anti-HBc is still a challenge. To elucidate the real importance of this finding in our blood donors, an investigation algorithm was tested. One hundred and twelve isolated anti-HBc seropositive blood donors underwent clinical evaluation and retesting of HBV markers. Those who presented repeatedly reactive isolated anti-HBc, received a single dose of hepatitis B recombinant vaccine to verify anti-HBs early response. A HBV-DNA determination by PCR was done for those who did not test positive to anti-HBs after vaccine. The level of anti-HBc was recorded as a ratio of the sample-to-cut-off values (S:C ratio in 57 candidates at donation. Comparing true and false-positive anti-HBc results, the different S:C ratios of them were statistically significant and when less than 2, implying in a false-positive result probability over 80%. A high percent of false-positive results (16.07% was verified after anti-HBc retesting. HBV immunity was characterized in 49.11%, either by anti-HBs detection in retesting (15.18%, or after a single dose HBV vaccination (33.93%. HBV-DNA was negative in all tested donors. In conclusion, this algorithm was useful to clarify the meaning of isolated anti-HBc in most of our blood donors.

  12. High frequencies of HGV and TTV infections in blood donors in Hangzhou

    Institute of Scientific and Technical Information of China (English)

    Jie Yan; Li-Li Chen; Yi-Hui LUO; Ya-Fei Mao; Meng He

    2001-01-01

    ALM To determine the frequencies of HGV and TTVinfections in blood donors in Hangzhou.METHODS RT-nested PCR for HGV RNA detection and semi-nested PCR for TTV DNA detection in the sera from 203 blood donors, and nucleotide sequence analysis were performed.``RESULTS Thirty-two ( 15.8%) and 30 (14.8%) of the 203serum samples were positive for HGV RNA and TTV DNA,respectively. And 5 (2.5%) of the 203 serum samples were detectable for both HGV RNA and TTV DNA.Homology of the nucleotide sequences of HGV RT-nested PCR products and TTV semi-nested PCR products from 3serum samples compared with the reported HGV and TTV sequences was 89.36%. 87.94%, 88.65% and 63.51%.65.77% and 67.12%. respectively.``CONCLUSION The infection rates of HGV and/or TTV inblood donors are relatively high. and to establish HGV and TTV examinations to screen blood donors is needed for transfusion security. The genomic heterogeneity of TTV or HGV is present in the isolates from different areas.``

  13. Risk factors from HBV infection among blood donors:A systematic review

    Institute of Scientific and Technical Information of China (English)

    Giuseppe La Torre; Rosella Saulle

    2016-01-01

    Objective:To perform a systematic review of the scientific literature to identify risk factors associated with hepatitis B viruses(HBV) infection among blood donors.Methods:The literature search was carried out on Pub Med and Scopus databases using the keywords "risk factors" "HBV infection" and "blood donors".No date or language restrictions were applied to the search.This literature review was completed in March2014.The selection process and the reporting of the review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Statement.The Newcastle Ottawa scale was using to evaluate the quality of each single primary study.Results:Out of 172 records resulted in the search,5 papers were included in the final analysis because they are within acceptance criteria.Two of the selected studies were cross-sectional and three of them were case-control studies.Significant association resulted with some demographic and behavioral risk factors,such as marital status,dental treatment/procedure history,no stable relationship or multiple partners and family history of HBV infection.Conclusions:The systematic review performed encourages to conduct further research among blood donors in order to fully understand risk factors among donors in more extensive thus to provide valuable information about surveillance.

  14. Patterns of infections among blood donors in a tertiary care centre: a retrospective study.

    Science.gov (United States)

    Kaur, Gagandeep; Basu, Sabita; Kaur, Ravneet; Kaur, Paramjit; Garg, Shailja

    2010-01-01

    Transfusion-transmitted infections continue to be a threat to safe transfusion practices. We analysed the prevalence and patterns of co-infections among voluntary and replacement donors. Blood donations collected over a 5-year period were studied for the type of donation (voluntary or replacement), number of seroreactive cases and the number, type and distribution of co-infections. Of the 42 439 units of blood collected over a 5-year period, 19 118 (45%) were from voluntary and 23 321 (55%) from replacement donors. There were 1603 seroreactive cases (3.8%). These included 250 with HIV (0.6%), 734 with hepatitis B surface antigen (HBsAg; 1.7%), 337 with hepatitis C virus (HCV; 0.8%) and 282 (0.7%) with VDRL (Venereal Diseases Research Laboratory) reactivity. Twenty-three (0.05%) of these had > or = 2 seroreactive infections; 20 of these were in replacement donors and only 3 in voluntary donors and the difference was statistically significant (p VDRL, 4 for HCV and VDRL, and 2 for HBsAg and HCV. One person was seroreactive for HIV, HBsAg and VDRL. The multiple infection rate showed a decreasing trend over the years. Multiple infections pose a small but definite risk to the recipients of blood products. Voluntary donations are safer as compared with replacement ones and need to be encouraged.

  15. Hepatitis B core antibody testing in Indian blood donors: A double-edged sword!

    Directory of Open Access Journals (Sweden)

    R N Makroo

    2012-01-01

    Full Text Available Background: Until lately, anti-HBc antibodies were considered an effective marker for occult Hepatitis B virus (HBV infection and have served their role in improving blood safety. But, with the development of advanced tests for HBV DNA detection, the role of anti-HBc in this regard stands uncertain. Materials and Methods: Anti-HBc and HBsAg ELISA and ID-NAT tests were run in parallel on donor blood samples between April 1, 2006 and December 31, 2010 at the Department of Transfusion Medicine, Indraprastha Apollo Hospitals, New Delhi. A positive ID-NAT was followed by Discriminatory NAT assay. Results: A total of 94 247 samples were tested with a total core positivity rate of 10.22%. We identified nearly 9.17% of donors who were reactive for anti-HBc and negative for HBsAg and HBV DNA. These are the donors who are potentially non-infectious and may be returned to the donor pool. Conclusion: Although anti HBc testing has a definite role in improving blood safety, centers that have incorporated NAT testing may not derive any additional benefit by performing anti-HBc testing, especially in resource-limited countries like ours.

  16. Incidence and clinical implication of TT virus in patients with hepatitis and its frequency in blood donors in India

    Science.gov (United States)

    Magu, S.K.; Kalghatgi, A.T.; Bhagat, M.R.

    2015-01-01

    Background Transfusion Transmitted Virus (TTV), also known as Torque Teno Virus is a new novel viral agent which appears to correlate with some acute and chronic hepatitis cases and may produce liver damage under specific circumstances. Aim of this study was to detect TT virus by real-time PCR, study its clinical implications and effects of its co-infection in HBV and HCV chronic liver diseases. Methods The study population comprised 50 acute hepatitis, 50 chronic hepatitis patients and 100 voluntary blood donors. All samples were tested for serum bilirubin, AST, ALT and alkaline phosphatase levels and for all available viral markers for hepatitis. The detection of TT viral genome was carried out by real-time PCR using TTV sequences as reported by Takahashi et al with modifications on the basis of database of the DDBJ/EMBL/GenBank (GenBank accession no. AB008394). Result Serum was positive for TTV in 72% of volunteer blood donors, 77.4% (24/31) of hepatitis A cases, 87.6% (36/41) of HBV-positive, 77% (10/13) of HCV-positive, and 92.8% (13/14) of non-B, non-C cases. Co-infection of TTV with other hepatitis viruses was detected in some patients. Conclusion TTV is a frequent virus detected in patients with various types of viral hepatitis, in cases of hepatitis without obvious viral agent, and from the healthy population in India. Rate of TTV was found to be significantly higher (92.8%) for Non A–E hepatitis group. PMID:26663961

  17. OCCULT HEPATITIS B VIRUS INFECTION AMONG BLOOD DONORS WITH ANTIBODIES TO HEPATITIS B CORE ANTIGEN

    Directory of Open Access Journals (Sweden)

    A. Jafarzadeh

    2008-04-01

    Full Text Available Diagnosis of hepatitis B is routinely based on of serological assay of hepatitis B surface antigen (HBsAg. Occult hepatitis B virus (HBV infection is generally defined as the detection of HBV -DNA in the serum or tissues of subjects who have negative test for HBsAg. Transmission of HBV infection has been documented from HBsAg negative, anti-HBc positive blood and organ donors. The aim of this study was to determine the rate of occult HBV infection among HBsAg negative and anti-HBc positive blood donors of Rafsanjan blood transfusion center. ‎ Sera from 270 healthy blood donors who were negative for both HBsAg and anti-HCV, were tested for anti-HBc antibodies by use of ELISA technique. The samples that were negative for HBsAg but positive for anti-HBc markers also examined for the presence of HBV-DNA by polymerase chain reaction (PCR. ‎ Out of 270 HBsAg negative blood samples, 14 samples (5.18% were positive for anti-HBc antibodies. HBV-DNA was detected in 4/14 (28.57% of HBsAg negative and anti-HBc positive samples. Moreover, anti-HBs antibody was detected in 2/4 (50% of HBV-DNA positive samples. ‎ These results indicated that HBV-DNA found in the majority of HBsAg negative and anti-HBc-positive donors. In addition, the present study recommend the incorporation of routine anti-HBc screening of blood as a surrogate marker of occult HBV infection to prevent some transfusion-transmitted HBV infections.

  18. Seroepidemiology of Toxoplasma gondii Infection among healthy blood donors in Taiwan.

    Directory of Open Access Journals (Sweden)

    Ting-Yi Chiang

    Full Text Available Toxoplasma gondii is an opportunistic, zoonotic pathogen with a worldwide distribution. There are large variations in the seroprevalence of T. gondii infection in different regions of the world. Although toxoplasmosis became a notifiable communicable disease in Taiwan in 2007, little is known about its epidemiology among the general population. This cross-sectional study aimed to survey the seroprevalence of T. gondii infection and its risk factors among healthy blood donors in Taiwan. Through collaborating with the Taiwan Blood Services Foundation, a total of 1,783 healthy blood donors from all six-branch blood service centers participated in this study. The blood samples were tested for the presence of T. gondii antibodies and DNA using enzyme immunoassays and real-time PCR, respectively. Structured questionnaires were used to gather information on risk factors for T. gondii infection. Of the 1,783 participants, 166 (9.3% tested positive for anti-Toxoplasma IgG, while 5 (0.28% tested positive for anti-Toxoplasma IgM. The five IgM positive donors had high avidity antibodies suggestive of past infection. No active parasitemia was detected by real-time PCR assays. Multivariate logistic regression showed that undercooked pork meat consumption (adjusted odds ratio [OR] = 2.9; 95% confidence interval [CI]: 1.3-6.5, raw mussels consumption (adjusted OR = 5.3; 95% CI: 1.5-19.1, having a cat in the household (adjusted OR = 2.0; 95% CI: 1.2-3.2, a lower education level (adjusted OR = 1.6; 95% CI: 1.1-2.3, and donation place in eastern Taiwan (adjusted OR = 2.5; 95% CI: 1.6-3.9 were independent risk factors for Toxoplasma seropositivity. These findings provide information on the seroprevalence and epidemiology of T. gondii infection among healthy blood donors in Taiwan.

  19. Virological and epidemiological features of hepatitis delta infection among blood donors in Nouakchott, Mauritania.

    Science.gov (United States)

    Mansour, Wael; Bollahi, Mohamed-Abdellahi; Hamed, Cheikh-Tijani; Brichler, Ségolène; Le Gal, Frédéric; Ducancelle, Alexandra; Lô, Baidy; Gordien, Emmanuel; Rosenheim, Michel; Lunel, Françoise

    2012-09-01

    In Mauritania, some authors have described a possible high prevalence of hepatitis delta virus (HDV) infection in the 1990s in studies of small-size samples. The aims of our study were to assess the prevalence of HDV in HBsAg positive blood donors in Mauritania, to identify the main risk factors for HDV transmission and to analyze genetic diversity of HDV strains. From October 2008 to December 2009, 11,100 consecutive blood donors were considered in this study. Among them, 1700 (15.3%) were HBsAg positive and 455 accepted to participate in this study. Demographic, epidemiological, ethnical, clinical and biological data were recorded. HDV screening, i.e., antibodies (HDVAb) and RNA (HDV-RNA) detection, was performed for all of them as well as HDV and HBV genotyping. Ninety/455 (19.78%) donors were HDVAb positive and HDV-RNA was detectable in 56 (62.2%) of them. HDV infection was significantly associated with older age, number of marriages, military profession, residence in the desert and a history of hospitalization. The HDV genotypes of the circulating strains were HDV-1 (89.3%) and HDV-5 (10.7%). HDV is highly endemic in Mauritanian blood donors indicating that a high number of them will develop chronic hepatitis, cirrhosis or hepatocellular carcinoma. Associated risk factors support nosocomial transmission of HDV. These data underline the need to reinforce HBV vaccination in newborns and in blood donors without HBV markers, together with screening for HDV in HBV-infected individuals. Copyright © 2012 Elsevier B.V. All rights reserved.

  20. Anti-HBc screening in Indian blood donors: Still an unresolved issue

    Science.gov (United States)

    Dhawan, Hari Krishan; Marwaha, Neelam; Sharma, Ratti Ram; Chawla, Yogesh; Thakral, Beenu; Saluja, Karan; Sharma, Sanjeev Kumar; Thakur, Manish K; Jain, Ashish

    2008-01-01

    AIM: To study the seroprevalence of antibody to hepatitis B core antigen (anti-HBc) in healthy blood donors negative for HBsAg and to evaluate whether anti-HBc detection could be adopted in India as a screening assay for HBV in addition to HBsAg. METHODS: A total of 1700 serum samples collected from HBsAg-negative healthy blood donors were tested for the presence of anti-HBc antibody (IgM + IgG). All samples reactive for anti-HBc antibody were then investigated for presence of anti-HBs and for liver function tests (LFTs). One hundred serum samples reactive for anti-HBc were tested for HBV DNA by PCR method. RESULTS: Out of 1700 samples tested, 142 (8.4%) blood samples were found to be reactive for anti-HBc. It was significantly lower in voluntary (6.9%) as compared to replacement donors (10.4%, P = 0.011). Seventy-two (50.7%) anti-HBc reactive samples were also reactive for anti-HBs with levels > 10 mIU/mL and 70 (49.3%) samples were non-reactive for anti-HBs, these units were labeled as anti-HBc-only. These 142 anti-HBc reactive units were also tested for liver function test. HBV DNA was detected in only 1 of 100 samples tested. CONCLUSION: Keeping in view that 8%-18% of donor population in India is anti-HBc reactive, inclusion of anti-HBc testing will lead to high discard rate. Anti-HBs as proposed previously does not seem to predict clearance of the virus. Cost effectiveness of introducing universal anti-HBc screening and discarding large number of blood units versus considering ID NAT (Individual donor nuclic acid testing) needs to be assessed. PMID:18785287

  1. Anti-HBc screening in Indian blood donors: Still an unresolved issue

    Institute of Scientific and Technical Information of China (English)

    Hari Krishan Dhawan; Neelam Marwaha; Ratti Ram Sharma; Yogesh Chawla; Beenu Thakral; Karan Saluja; Sanjeev Kumar Sharma; Manish K Thakur; Ashish Jain

    2008-01-01

    AIM:To study the seroprevalence of antibody to hepatitis B core antigen (anti-HBc) in healthy blood donors negative for HBsAg and to evaluate whether anti-HBc detection could be adopted in India as a screening assay for HBV in addition to HBsAg.METHODS:A total of 1700 serum samples collected from HBsAg-negative healthy blood donors were tested for the presence of anti-HBc antibody (IgM + IgG).All samples reactive for anti-HBc antibody were then investigated for presence of anti-HBs and for liver function tests (LFTs).One hundred serum samples reactive for anti-HBc were tested for HBV DNA by PCR method.RESULTS:Out of 1700 samples tested,142 (8.4%)blood samples were found to be reactive for anti-HBc.It was significantly lower in voluntary (6.9%) as compared to replacement donors (10.4%,P=0.011).Seventytwo (50.7%) anti-HBc reactive samples were also reactive for anti-HBs with levels >10 mIU/mL and 70(49.3%) samples were non-reactive for anti-HBs,these units were labeled as anti-HBc-only.These 142 anti-HBc reactive units were also tested for liver function test.HBV DNA was detected in only 1 of 100 samples tested.CONCLUSION:Keeping in view that 8%-18% of donor population in India is anti-HBc reactive,inclusion of anti-HBc testing will lead to high discard rate.Anti-HBs as proposed previously does not seem to predict clearance of the virus.Cost effectiveness of introducing universal anti-HBc screening and discarding large number of blood units versus considering ID NAT (Individual donor nuclic acid testing) needs to be assessed.

  2. Donor Tag Game

    Science.gov (United States)

    ... Donor Community > Games > Donor Tag Game Donor Tag Game This feature requires version 6 or later of ... of Needles LGBTQ+ Donors Blood Donor Community SleevesUp Games Facebook Avatars and Badges Banners eCards Make a ...

  3. Prevalence of Hepatitis C Virus Genotypes in Asymptomatic Blood Donors in NCT of Delhi

    OpenAIRE

    2015-01-01

    Hepatitis C virus shows high genomic diversity which poses a challenge for drug therapy and vaccine development. Here, we present a study on prevalence of HCV genotypes and subtypes in blood donors of Delhi. Forty two HCV samples were taken from blood bags found positive at various blood banks located in Delhi. From these samples 5’UTR were amplified, sequenced and genotyped. Genotype 1, 3 and 4 were detected but genotype 1 of HCV was found prevalent. Based on sequence analysis the HCV isolat...

  4. TT virus and hepatitis G virus infections in Korean blood donors and patients with chronic liver disease

    Institute of Scientific and Technical Information of China (English)

    Mee Juhng Jeon; Jong Hee Shin; Soon Pal Suh; Yong Chai Lim; Dong Wook Ryang

    2003-01-01

    AIM: To determine the prevalences of TTV and HGV infections among blood donors and patients with chronic liver disease in Korea, to investigate the association of TTV and HGV infections with blood transfusion, and to assess the correlation between TTV and HGV viremia and hepatic damage.METHODS: A total of 391 serum samples were examined in this study. Samples were obtained from healthy blood donors (n= 110), hepatitis B surface antigen (HBsAg)-positive donors (n=112), anti-hepatitis C virus (anti-HCV)-positive donors (n=69), patients with type B chronic liver disease (n=81), and patients with type C chronic liver disease (n= 19).TTV DNA was detected using the hemi-nested PCR. HGV RNA was tested using RT-PCR. A history of blood transfusion and serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were also determined.RESULTS: TTV DNA was detected in 8.2 % of healthy blood donors, 16.1% of HBsAg-positive donors, 20.3 % of antiHCV-positive donors, 21.0 % of patients with type B chronic liver disease, and 21.1% of patients with type C chronic liver disease. HGV RNA was detected in 1.8 % of healthy blood donors, 1.8 % of HBsAg-positive donors, 17.4 % of anti-HCV-positive donors, 13.6% of patients with type B chronic liver disease, and 10.5% of patients with type C chronic liver disease. The prevalence of TTV and HGV infections in HBV- or HCV-positive donors and patients was significantly higher than in healthy blood donors (P<0.05),except for the detection rate of HGV in HBsAg-positive donors which was the same as for healthy donors. There was a history of transfusion in 66.7% of TTV DNA-positive patients and 76.9% of HGV RNA-positive patients (P<0.05). No significant increase in serum ALT and AST was detected in the TTV- or HGV-positive donors and patients.CONCLUSION: TTV and HGV infections are more frequently found in donors and patients infected with HBV or HCV than in healthy blood donors. However, there is no significant

  5. High prevalence of HIV p24 antigen among HIV antibody negative prospective blood donors in Ile-Ife, Nigeria.

    Science.gov (United States)

    Japhet, Margaret Oluwatoyin; Adewumi, Moses Olubusuyi; Adesina, Olufisayo Adeyemi; Donbraye, Emmanuel

    2016-01-01

    Blood transfusion service centers in Nigeria screen donated blood for markers of HIV infection using antibody- (Ab) based rapid test and in some centers, positives are re-tested using Ab-based ELISA. Paucity of data exists on p24 antigen prevalence among HIV Ab-negative donors in Nigeria. This study aims at detecting HIV p24 antigen among prospective blood donors in Osun State, Nigeria. Prospective blood donors negative for HIV antibodies using Determine test kit were re-tested using BIORAD GENSCREEN Ultra Ag-Ab ELISA kit, a fourth-generation ELISA kit that detects HIV antibodies/p24 antigen. Of the 169 HIV Ab-negative prospective donors, 10 (5.9%) were positive for HIV p24 antigen and 70% (7/10) of them were in the age range 18-30 years. Results of this study show that blood transfusion is still one of the major routes of HIV transmission in Nigeria and a higher proportion is among youth. Inclusion of p24 antigen testing into the blood donor screening will help reduce transfusion associated HIV in Nigeria if Nucleic Acid Testing (NAT) of all blood donor samples is not affordable; also, HIV enlightenment programs tailored toward youth may help reduce this rate among donors since more young people donate blood in low/middle-income countries than in high-income countries.

  6. Prevalence of RhD variants among blood donors at Gulu Regional Blood Bank, Gulu, Northern Uganda

    Directory of Open Access Journals (Sweden)

    Ojok P

    2017-09-01

    Full Text Available Polycarp Ojok,1,2 Caesar Oyet,1 Fred Webbo,1,3 Bashir Mwambi,1 Ivan M Taremwa1 1Institute of Allied Health Sciences, International Health Sciences University, Kampala, 2Gulu Regional Blood Bank, Gulu, 3Lancet Laboratories, Kampala, Uganda Aim/objective: The aim of this study was to determine the prevalence of RhD variant ­phenotypes among voluntary non-remunerated blood donors (VNRBDs at Gulu Regional Blood Bank (GRBB, Northern Uganda. Materials and methods: We conducted a cross-sectional study, in which the first 4.0 mL of ethylenediaminetetraacetic acid (EDTA blood samples were collected from VNRBDs and typed for their ABO and RhD blood group status using IgM and IgG monoclonal typing antisera, respectively. Blood samples that tested as RhD negative were further investigated for RhD variant phenotypes using indirect antihuman globulin hemagglutination technique. Results: We assayed 138 RhD-negative blood samples obtained from VNRBDs. Of these, 66.7% (n=92 were males. Their median age was 24.4 years (range, 14–33 years. Majority of the participants were of ABO blood group O (62.8%, n=86, followed by A (19.7%, n=27, then B (13.9%, n=19 and least AB (3.6%, n=6. The prevalence of RhD variant phenotypes was 0.7% (n=1; 95% confidence interval, 0.5–0.9. There was no statistical association of RhD variant phenotypes with donor gender, tribe and their ABO blood groups. Conclusion: This study has revealed a high prevalence of RhD variant among blood donors at GRBB in Northern Uganda. It further highlights a potential risk of alloimmunization, as the present blood typing practices do not identify RhD variant phenotypes. Keywords: Rh blood group, D variants, D antigen, weak D, partial D, Uganda 

  7. [Seroprevalence of HTLV-1/2 in blood donors from Misiones Province].

    Science.gov (United States)

    Malan, Richard; Berini, Carolina A; Eirin, María E; Delfino, Cecilia M; Pedrozo, Williams; Krupp, Ramón; García Plichta, Atilio; Biglione, Mirna M

    2010-01-01

    Human T-cell Lymphotropic viruses type 1 (HTLV-1), the first human oncoretrovirus to be discovered, is the etiologic agent of Adult T-cell Leukemia (ATL) and HTLV-1 Associated Mielopathy or Tropical Spastic Paraparesis (HAM/TSP). It is endemic worldwide, including the North of Argentina where both associated diseases have also been detected. No etiologic role has been described for HTLV-2, although it has been associated with HAM/TSP-like neurologic syndromes. Both retroviruses are endemic in native populations of The Americas, Africa and at-risk populations. They are transmitted through sex contact, parenterally and from mother to child. The aim of this study was to estimate the seroprevalence of HTLV-1/2 in a blood donor population from Misiones province. A total of 6912 accepted blood donations in 2008 were analyzed. HTLV-1/2 screening was performed with ELISA and particle agglutination, and reactive samples were confirmed by Western Blot. From the total, 5 samples resulted seropositive with a final prevalence of 0.00072. Out of the 5 positive samples, one was an HTLV, three HTLV-1 and one HTLV-2. These blood donors were residents of Posadas, Eldorado and Oberá, with no risk antecedents. This study demonstrates the presence of HTLV-1/2 in a population of Misiones with a prevalence rate similar to those reported among blood donors from non-endemic areas.

  8. HTLV-I/II and blood donors: determinants associated with seropositivity in a low risk population

    Directory of Open Access Journals (Sweden)

    Bernadette Catalan Soares

    2003-08-01

    Full Text Available OBJECTIVE: Blood donors in Brazil have been routinely screened for HTLV-I/II since 1993. A study was performed to estimate the prevalence of HTLV-I/II infection in a low risk population and to better understand determinants associated with seropositivity. METHODS: HTLV-I/II seropositive (n=135, indeterminate (n=167 and seronegative blood donors (n=116 were enrolled in an open prevalence prospective cohort study. A cross-sectional epidemiological study of positive, indeterminate and seronegative HTLV-I/II subjects was conducted to assess behavioral and environmental risk factors for seropositivity. HTLV-I/II serological status was confirmed using enzyme-linked immunosorbent assay (EIA and Western blot (WB. RESULTS: The three groups were not homogeneous. HTLV-I/II seropositivity was associated to past blood transfusion and years of schooling, a marker of socioeconomic status, and use of non-intravenous illegal drugs. CONCLUSIONS: The study results reinforce the importance of continuous monitoring and improvement of blood donor selection process.

  9. Rh phenotype, allele and haplotype frequencies among 51,857 blood donors in North India.

    Science.gov (United States)

    Makroo, Raj; Gupta, Richa; Bhatia, Aakanksha; Rosamma, Nakamatathil L

    2014-01-01

    This study was performed to provide information on the frequencies of Rh antigens, alleles, phenotypes, and haplotypes from our region in India and to compare them with those from other races. This observational study was conducted on blood donors from March 2009 to August 2011 using a fully automated system for Rh typing of blood cells. The data were collected and calculations done to determine the antigen, phenotypes, allele and haplotype frequencies. The chi square test was used for comparisons between the results of our study and those of other studies. A total of 51,857 donors were included in this study. The most common Rh antigen found was "e". DCCee was the most prevalent phenotype in our study with the phenotype distribution being significantly different between our study and other studies from different regions of the world. We have determined the prevalence of Rh antigens and Rh phenotypes in the North Indian blood donor population and derived the allele and haplotype frequencies in the same population. The Rh blood group distribution in this population was different from that in other populations.

  10. Hematopoietic SCT in Europe 2013: recent trends in the use of alternative donors showing more haploidentical donors but fewer cord blood transplants.

    Science.gov (United States)

    Passweg, J R; Baldomero, H; Bader, P; Bonini, C; Cesaro, S; Dreger, P; Duarte, R F; Dufour, C; Falkenburg, J H F; Farge-Bancel, D; Gennery, A; Kröger, N; Lanza, F; Nagler, A; Sureda, A; Mohty, M

    2015-04-01

    A record number of 39,209 HSCT in 34,809 patients (14,950 allogeneic (43%) and 19,859 autologous (57%)) were reported by 658 centers in 48 countries to the 2013 survey. Trends include: more growth in allogeneic than in autologous HSCT, increasing use of sibling and unrelated donors and a pronounced increase in haploidentical family donors when compared with cord blood donors for those patients without a matched related or unrelated donor. Main indications were leukemias, 11,190 (32%; 96% allogeneic); lymphoid neoplasias, 19,958 (57%; 11% allogeneic); solid tumors, 1543 (4%; 4% allogeneic); and nonmalignant disorders, 1975 (6%; 91% allogeneic). In patients without a matched sibling or unrelated donor, alternative donors are used. Since 2010 there has been a marked increase of 96% in the number of transplants performed from haploidentical relatives (802 in 2010 to 1571 in 2013), whereas the number of unrelated cord blood transplants has slightly decreased (789 in 2010 to 666 in 2013). The use of donor type varies greatly throughout Europe.

  11. Hepatitis B, HIV, and Syphilis Seroprevalence in Pregnant Women and Blood Donors in Cameroon

    Directory of Open Access Journals (Sweden)

    Jodie Dionne-Odom

    2016-01-01

    Full Text Available Objectives. We estimated seroprevalence and correlates of selected infections in pregnant women and blood donors in a resource-limited setting. Methods. We performed a cross-sectional analysis of laboratory seroprevalence data from pregnant women and voluntary blood donors from facilities in Cameroon in 2014. Rapid tests were performed to detect hepatitis B surface antigen, syphilis treponemal antibodies, and HIV-1/2 antibodies. Blood donations were also tested for hepatitis C and malaria. Results. The seroprevalence rates and ranges among 7069 pregnant women were hepatitis B 4.4% (1.1–9.6%, HIV 6% (3.0–10.2%, and syphilis 1.7% (1.3–3.8% with significant variability among the sites. Correlates of infection in pregnancy in adjusted regression models included urban residence for hepatitis B (aOR 2.9, CI 1.6–5.4 and HIV (aOR 3.5, CI 1.9–6.7. Blood donor seroprevalence rates and ranges were hepatitis B 6.8% (5.0–8.8%, HIV 2.2% (1.4–2.8%, syphilis 4% (3.3–4.5%, malaria 1.9%, and hepatitis C 1.7% (0.5–2.5%. Conclusions. Hepatitis B, HIV, and syphilis infections are common among pregnant women and blood donors in Cameroon with higher rates in urban areas. Future interventions to reduce vertical transmission should include universal screening for these infections early in pregnancy and provision of effective prevention tools including the birth dose of univalent hepatitis B vaccine.

  12. [Prevalence of antibodies against Trypanosoma cruzi in blood bank donors from the IMSS General Hospital in Orizaba, Veracruz, Mexico].

    Science.gov (United States)

    Ramos-Ligonio, Angel; Ramírez-Sánchez, Michaía Elián; González-Hernández, Juan Carlos; Rosales-Encina, José Luis; López-Monteon, Aracely

    2006-01-01

    To estimate the prevalence of antibodies against Trypanosoma cruzi in blood donors from Hospital General Regional (HGRO) of the Mexican Institute of Social Security (IMSS per its abbreviation in Spanish). Between October 2001 and January 2002, blood samples were collected from voluntary donors at the blood bank of the Hospital General Regional of IMSS in Orizaba; Veracruz, Mexico. The samples were assayed for anti-T. cruzi by ELISA, Western blot and IFI, using a recombinant protein (MBP::Hsp70), and crude extract from epimastigotes. A total of 420 blood donors were studied; two of them were seropositive for ELISA,Western blot and IFI, with a seroprevalence of 0.48%. Some blood donors at the HGRO hospital were seropositive for T. cruzi, showing the risk of contamination by blood transfusion. Routine serologic screening with highly sensitive and specific immunological techniques are needed.

  13. Phenotypic and allelic profile of ABO and Rhésus D blood group system among blood donor in Antananarivo.

    Science.gov (United States)

    Randriamanantany, Z A; Rajaonatahina, D H; Razafimanantsoa, F E; Rasamindrakotroka, M T; Andriamahenina, R; Rasoarilalamanarivo, F B; Hanitriniala, S P; Herisoa, F R; Rasamindrakotroka, A; Rakoto Alson, O A

    2012-12-01

    This study assessed the phenotypic and allelic profiles of ABO and Rhesus D blood group system among first time blood donors at the National Centre of Blood Supply of Antananarivo. We collected through this retrospective study all data registered during 7 years of practice (from 2003 to 2009). Age and sex were analysed with the result of ABO and RhD screening. They were tested both with Beth Vincent and Simonin tests which were performed in a plate, by using commercial monoclonal antibody (Diaclone(®) et Eryclone(®)), and home-made red cells tests. The Rh D was performed with the same commercial kits. The frequencies of alleles were calculated by using Bernstein method. Data about 45,857 donors were obtained. A male predominance (80.46%) was found and most of our donors were aged ABO antigen was, respectively, 22.61, 29.66, 6.13 and 41.60% for A, B, AB and O antigen. Allelic frequencies of A, B and O were 0.1559, 0.1987 and 0.6454. These results confirmed the fact that Madagascan population had admixed ethnic origin.

  14. Prevalence of Chagas disease in blood donors at the Uberaba Regional Blood Center, Brazil, from 1995 to 2009

    Directory of Open Access Journals (Sweden)

    Laryssa Manso de Lima

    2012-12-01

    Full Text Available INTRODUCTION: A retrospective study was conducted to assess the occurrence of blood donations that were ineligible due to Chagas disease infection from 1995 to 2009 at the Uberaba Regional Blood Center (HRU, Brazil, verify the tendency of this ineligibility, and describe the epidemiologic profile of the donors. METHODS: Retrospective studies of serological ineligibility due to Chagas disease, statistical analysis by means of the chi-square test and odds ratio, study of the tendencies using a dispersion graph and the linear correlation coefficient (r were performed. RESULTS: In the period under study, a 0.2% serum prevalence of ineligibility due to Chagas disease was found, with a significant drop in ineligible donations from 2001 to 2009. Among the serum positive-donors, there was a significant predominance among those aged 30 years or above and non-single individuals. CONCLUSIONS: The results show a rate of occurrence that is lower than that described in literature, as well as a progressive drop during the 15 years under assessment. Such results are a consequence of systematic combat of the vector since the 70s and the progressive and consistent increase of returning donors, resulting in a drop of the contamination risk factor by means of blood transfusion and in the improvement of the quality of hemotherapy practices in the HRU.

  15. Hepatitis C virus infection in blood donors from the state of Puebla, Mexico

    Directory of Open Access Journals (Sweden)

    Contreras-Mioni Laura

    2010-01-01

    Full Text Available Abstract Background Worldwide, 130 million persons are estimated to be infected with HCV. Puebla is the Mexican state with the highest mortality due to hepatic cirrhosis. Therefore, it is imperative to obtain epidemiological data on HCV infection in asymptomatic people of this region. The objective of present study was to analyze the prevalence of antibodies and genotypes of hepatitis C virus (HCV in blood donors from Puebla, Mexico. Results The overall prevalence was 0.84% (515/61553. Distribution by region was: North, 0.86% (54/6270; Southeast, 1.04% (75/7197; Southwest, 0.93% (36/3852; and Central, 0.79% (350/44234. Ninety-six donors were enrolled for detection and genotyping of virus, from which 37 (38.5% were HCV-RNA positive. Detected subtypes were: 1a (40.5%, 1b (27.0%, mixed 1a/1b (18.9%, undetermined genotype 1 (5.4%, 2a (2.7%, 2b (2.7%, and mixed 1a/2a (2.7%. All recovered donors with S/CO > 39 were HCV-RNA positive (11/11 and presented elevated ALT; in donors with S/CO Conclusions HCV prevalence of donors in Puebla is similar to other Mexican states. The most prevalent genotype is 1, of which subtype 1a is the most frequent.

  16. Occult hepatitis B virus infection among blood donors from the Brazilian Amazon: implications for transfusion policy

    Science.gov (United States)

    Moresco, M. N. dos S.; Virgolino, H. de A.; de Morais, M. P. E.; da Motta-Passos, I.; Gomes-Gouvêa, M. S.; de Assis, L. M. S.; Aguiar, K. R. de L.; Lombardi, S. C. F.; Malheiro, A.; Cavalheiro, N. de P.; Levi, J. E.; Torres, K. L.

    2016-01-01

    Background Brazil requires the performance of both a test for hepatitis B surface antigen (HBsAg) and a test for antibodies to the core of hepatitis B for blood donor screening. Blood centres in regions of high HBV endemicity struggle to maintain adequate stocks in face of the high discard rates due to anti-HBc reactivity. We evaluated the potential infectivity of donations positive for anti-HBc in search of a rational approach for the handling of these collections. Study Design and Methods We tested anti-HBc reactive blood donations from the state of Amazonas for the presence of HBV DNA and for titres of anti-HBs. The study population consists of village-based donors from the interior of Amazonas state. Results Among 3600 donations, 799 were anti-HBc reactive (22·2%). We were able to perform real-time PCR for the HBV S gene on specimens from 291 of these donors. Eight of these samples were negative for HBsAg and positive for HBV DNA and were defined as occult B virus infections (2·7%). Six of those eight specimens had anti-HBs titres above 100 mIU/ml, indicating the concomitant presence of the virus with high antibody titres. Conclusion A small proportion of anti-HBc reactive donors carry HBV DNA and anti-HBs testing is not useful for predicting viremia on them. This finding indicates the possibility of HBV transmission from asymptomatic donors, especially in areas of high HBV prevalence. Sensitive HBV DNA nucleic acid testing may provide another level of safety, allowing eventual use of anti-HBc reactive units in critical situations. PMID:24697276

  17. Risk Factors for Bartonella species Infection in Blood Donors from Southeast Brazil.

    Science.gov (United States)

    Diniz, Pedro Paulo Vissotto de Paiva; Velho, Paulo Eduardo Neves Ferreira; Pitassi, Luiza Helena Urso; Drummond, Marina Rovani; Lania, Bruno Grosselli; Barjas-Castro, Maria Lourdes; Sowy, Stanley; Breitschwerdt, Edward B; Scorpio, Diana Gerardi

    2016-03-01

    Bacteria from the genus Bartonella are emerging blood-borne bacteria, capable of causing long-lasting infection in marine and terrestrial mammals, including humans. Bartonella are generally well adapted to their main host, causing persistent infection without clinical manifestation. However, these organisms may cause severe disease in natural or accidental hosts. In humans, Bartonella species have been detected from sick patients presented with diverse disease manifestations, including cat scratch disease, trench fever, bacillary angiomatosis, endocarditis, polyarthritis, or granulomatous inflammatory disease. However, with the advances in diagnostic methods, subclinical bloodstream infection in humans has been reported, with the potential for transmission through blood transfusion been recently investigated by our group. The objective of this study was to determine the risk factors associated with Bartonella species infection in asymptomatic blood donors presented at a major blood bank in Southeastern Brazil. Five hundred blood donors were randomly enrolled and tested for Bartonella species infection by specialized blood cultured coupled with high-sensitive PCR assays. Epidemiological questionnaires were designed to cover major potential risk factors, such as age, gender, ethnicity, contact with companion animals, livestock, or wild animals, bites from insects or animal, economical status, among other factors. Based on multivariate logistic regression, bloodstream infection with B. henselae or B. clarridgeiae was associated with cat contact (adjusted OR: 3.4, 95% CI: 1.1-9.6) or history of tick bite (adjusted OR: 3.7, 95% CI: 1.3-13.4). These risk factors should be considered during donor screening, as bacteremia by these Bartonella species may not be detected by traditional laboratory screening methods, and it may be transmitted by blood transfusion.

  18. Risk Factors for Bartonella species Infection in Blood Donors from Southeast Brazil.

    Directory of Open Access Journals (Sweden)

    Pedro Paulo Vissotto de Paiva Diniz

    2016-03-01

    Full Text Available Bacteria from the genus Bartonella are emerging blood-borne bacteria, capable of causing long-lasting infection in marine and terrestrial mammals, including humans. Bartonella are generally well adapted to their main host, causing persistent infection without clinical manifestation. However, these organisms may cause severe disease in natural or accidental hosts. In humans, Bartonella species have been detected from sick patients presented with diverse disease manifestations, including cat scratch disease, trench fever, bacillary angiomatosis, endocarditis, polyarthritis, or granulomatous inflammatory disease. However, with the advances in diagnostic methods, subclinical bloodstream infection in humans has been reported, with the potential for transmission through blood transfusion been recently investigated by our group. The objective of this study was to determine the risk factors associated with Bartonella species infection in asymptomatic blood donors presented at a major blood bank in Southeastern Brazil. Five hundred blood donors were randomly enrolled and tested for Bartonella species infection by specialized blood cultured coupled with high-sensitive PCR assays. Epidemiological questionnaires were designed to cover major potential risk factors, such as age, gender, ethnicity, contact with companion animals, livestock, or wild animals, bites from insects or animal, economical status, among other factors. Based on multivariate logistic regression, bloodstream infection with B. henselae or B. clarridgeiae was associated with cat contact (adjusted OR: 3.4, 95% CI: 1.1-9.6 or history of tick bite (adjusted OR: 3.7, 95% CI: 1.3-13.4. These risk factors should be considered during donor screening, as bacteremia by these Bartonella species may not be detected by traditional laboratory screening methods, and it may be transmitted by blood transfusion.

  19. Demographic, risk factors and motivations among blood donors with reactive serologic tests for syphilis in São Paulo, Brazil.

    Science.gov (United States)

    Ferreira, S C; de Almeida-Neto, C; Nishiya, A S; Oliveira, C D L; Ferreira, J E; Alencar, C S; Levi, J E; Salles, N A; Mendrone, A; Sabino, E C

    2014-06-01

    To identify the demographic characteristics, risk factors and motivations for donating among blood donors with reactive serologic tests for syphilis. Post-donation interviews with syphilis seropositive blood donors improve recruitment and screening strategies. This case-control study compares 75 Venereal Disease Research Laboratory (VDRL) > 8, EIA+ (enzyme immunoassay) and FTA-ABS+ (fluorescent treponemal antibody); 80 VDRL-, EIA+ and FTA-ABS+; and 34 VDRL- and EIA- donors between 2004 and 2009. Donors were assessed by their demographic characteristics, sexual behaviour, history of alcohol and illicit drugs use, and motivations to donate. Donors with VDRL > 8 were more likely to be divorced [AOR = 12·53; 95% confidence interval (CI) 1·30-120·81], to have had more than six sexual partners (AOR=7·1; 95% CI 1·12-44·62) and to report male-male-sex in the past 12 months (AOR=8·18; 95% CI 1·78-37·60). Donors with VDRL-, EIA+ and FTA-ABS+ were less likely to be female (AOR=0·26; 95% CI 0·07-0·96), more likely to be older (AOR=10·2; 95% CI 2·45-42·58 ≥ 39 and VDRL > 8) and 12·5% (VDRL-, EIA+ and FTA-ABS+) of donors reported that they had been at risk for HIV infection (P = 0·004). One-third of donors came to the blood bank to help a friend or a relative who needed blood. Although donors exposed to syphilis reported and recognised some high risk behaviour, most were motivated by direct appeal to donate blood. Monitoring the risk profile of blood donors can benefit public health and improve blood safety. © 2014 The Authors. Transfusion Medicine © 2014 British Blood Transfusion Society.

  20. ALTERATIONS OF PROPERTIES OF RED BLOOD CELLS MEMBRANES PROTEINS OF DIFFERENT AGE AND SEX VOLUNTEERS.

    Science.gov (United States)

    Pruidze, N; Khetsuriani, R; Sujashvili, R; Ioramashvili, I; Arabuli, M; Sanikidze, T

    2015-01-01

    Considering the age and sex-dependent trend in the manifestation of various diseases, as well as an important pathogenic role of circulatory disorders, we decided to study the age-dependent changes in the physical properties of RBCs membrane proteins (their electric charge and molecular weight) in healthy people of different sex (males and females) and age. Blood of 56 healthy volunteers (Tbilisi, Georgia) of different sex and gender was studied (the patients were divided in 8 groups (7 patients in each groups): 1 - 18-25 years old male, 2 - 18-25 years old female, 3 - 25-44 years old male, 4 - 25-44 years old female, 5 - 44-60 years old male, 6 - 44-60 years old female; 7 - 60-80 years old male, 8 - 70-80 years old female). In groups 6 and 8 were women in menopause was determined according 12 months of amenorrhea. Individuals often consume alcohol addicts, pregnant women and patients with chronic diseases were excluded from the study. The study protocol was approved by Ethical Committee of the Tbilisi State Medical University. RBCs membrane proteins have been extracted from human heparinized blood and their mobility was studied by electrophoretic method. The electrophoretic mobility of RBCs membrane proteins decreases with age of healthy volunteers, that indicates decrease of total charge of proteins, depending on the electrically charged amino acids content. In female patients the electrophoretic mobility of the RBCs membrane proteins especially intensively decreases in period of menopause. Increase of molecular weight of proteins (100-200 kDa) from RBCs' membranes of alder age group was manifested. Intensively decrease electrophoretic mobility of erythrocytes membrane proteins from female patients in period of menopause indicates on estrogen related mechanism of the regulation of membrane protein conformation and composition in females. Increased content of high molecular weight proteins in the RBCs membranes from patients of older age groups may be caused to

  1. Tissue perfusion in neonates undergoing open-heart surgery using autologous umbilical cord blood or donor blood components.

    Science.gov (United States)

    Chasovskyi, K; Fedevych, O; McMullan, D M; Mykychak, Y; Vorobiova, G; Zhovnir, V; Yemets, I

    2015-09-01

    This study evaluates the hemoglobin-oxygen relationship and tissue perfusion during cardiopulmonary bypass (CPB) in neonates undergoing open-heart surgery using autologous umbilical cord blood or donor blood components. We compared perioperative hematocrit (Hct), fetal hemoglobin (HbF), p(50)O(2), pH, pCO(2), serum lactate, duration of mechanical ventilation and intensive care unit (ICU) length of stay in neonates undergoing open-heart surgery using autologous umbilical cord blood (Group I, N=45) or donor blood components (Group II, N=65). The groups were similar with respect to diagnosis, weight, type of procedure, duration of CPB and duration of myocardial ischemia. Mean p(50)O(2) was significantly lower in Group I during CPB (19.7 vs. 22.3 mmHg, p=0.004) and at the end of CPB (20.1 vs. 22.8 mmHg, p=0.003). Median peak lactate during CPB was higher in Group I (4.8 vs. 2.2 mmol/l, pblood, tissue oxygen delivery appears to be preserved in neonates who undergo open-heart surgery using autologous umbilical cord blood. © The Author(s) 2014.

  2. Thrombin generation during collection of blood from donors taking oral contraceptives.

    Science.gov (United States)

    Skjønsberg, O H; Kierulf, P; Engebretsen, L F; Gjønnes, G; Godal, H C

    1987-01-01

    Thrombin generation, as evidenced by plasma fibrinopeptide A (FPA) concentrations, was studied during blood collection from donors taking oral contraceptives (OC). 450 ml blood were drawn into Fenwal PVC bags from 26 OC users and 28 nonusers. Blood samples for determination of FPA, beta-thromboglobulin (BTG), thrombotest (TT), prekallikrein (PKK), antithrombin-III (AT-III) and factor VIII procoagulant activity (FVIII:C) were drawn from the bags immediately after ending blood donation and following storage for 24 h at 4 degrees C. The FPA concentrations following donation were significantly higher in the OC than in the control group (p less than 0.05). The levels of PKK were also higher in blood obtained from OC users (p less than 0.001), as was the FVIII:C level, the latter difference, however, was not significant (p = 0.06). No cold-promoted activation of factor VII, as evidenced from TT, was detected following storage at 4 degrees C, neither was any change observed in the FPA, PKK and AT-III levels. The BTG concentrations increased significantly during storage, most pronounced in the control group (p less than 0.05). The decay of FVIII:C was similar in the two groups, averaging 24.7%. No correlation was observed between the FPA levels and the other parameters determined. We conclude that thrombin generation is more pronounced during routine blood collection from donors taking OC.

  3. Human immunodeficiency virus (HIV) seropositivity and hepatitis B surface antigenemia (HBSAG) among blood donors in Benin city, Edo state, Nigeria.

    Science.gov (United States)

    Umolu, Patience Idia; Okoror, Lawrence Ehis; Orhue, Philip

    2005-03-01

    Human Immunodeficiency Virus and Hepatitis B virus are blood borne pathogens that can be transmitted through blood transfusion and could pose a huge problem in areas where mechanisms of ensuring blood safety are suspect. This study became necessary in a population where most of the blood for transfusion is from commercial blood donors. A total of 130 donors comprising 120 commercial donors and 10 voluntary donors were tested for antibodies to human immunodeficiency virus and hepatitis B surface antigen in Benin city using Immunocomb HIV - 1 and 2 Biospot kit and Quimica Clinica Aplicada direct latex agglutination method respectively. Thirteen (10%) samples were HIV seropositive and 7(5.8%) were HBsAg positive. The age bracket 18 - 25years had the highest numbers of donors and also had the highest number of HBsAg positive cases (7.8%) while the age group 29 - 38years had highest number of HIV seropositive cases. High prevalence of HIV antibodies and Hepatitis B surface antigen was found among commercial blood donors. Appropriate and compulsory screening of blood donors using sensitive methods, must be ensured to prevent post transfusion hepatitis and HIV.

  4. Assessing ABO/Rh Blood Group Frequency and Association with Asymptomatic Malaria among Blood Donors Attending Arba Minch Blood Bank, South Ethiopia

    Directory of Open Access Journals (Sweden)

    Getaneh Alemu

    2016-01-01

    Full Text Available Background. Determination of the various ABO/Rh blood group distributions and their association with malaria infection has paramount importance in the context of transfusion medicine and malaria control. Methods. Facility based cross-sectional study was conducted from February to June, 2015, to assess ABO/Rh blood groups distribution and their association with asymptomatic malaria. A structured questionnaire was used to collect data. Blood grouping was done using monoclonal antibodies. Thin and thick blood films were examined for Plasmodium parasites. Data were analyzed using SPSS version 20.0. Results. A total of 416 blood donors participated with median age of 22±0.29 (median ± standard error of the mean. Distribution of ABO phenotypes, in decreasing order, was O (175, 42.1%, A (136, 32.7%, B (87, 20.9%, and AB (18, 4.3%. Most of them were Rh+ (386, 92.8%. The overall malaria prevalence was 4.1% (17/416. ABO blood group is significantly associated with malaria infection (P=0.022. High rate of parasitemia was seen in blood group O donors (6.899, P=0.003 compared to those with other ABO blood groups. Conclusion. Blood groups O and AB phenotypes are the most and the least ABO blood groups, respectively. There is significant association between ABO blood group and asymptomatic malaria parasitemia.

  5. Assessing ABO/Rh Blood Group Frequency and Association with Asymptomatic Malaria among Blood Donors Attending Arba Minch Blood Bank, South Ethiopia.

    Science.gov (United States)

    Alemu, Getaneh; Mama, Mohammedaman

    2016-01-01

    Background. Determination of the various ABO/Rh blood group distributions and their association with malaria infection has paramount importance in the context of transfusion medicine and malaria control. Methods. Facility based cross-sectional study was conducted from February to June, 2015, to assess ABO/Rh blood groups distribution and their association with asymptomatic malaria. A structured questionnaire was used to collect data. Blood grouping was done using monoclonal antibodies. Thin and thick blood films were examined for Plasmodium parasites. Data were analyzed using SPSS version 20.0. Results. A total of 416 blood donors participated with median age of 22 ± 0.29 (median ± standard error of the mean). Distribution of ABO phenotypes, in decreasing order, was O (175, 42.1%), A (136, 32.7%), B (87, 20.9%), and AB (18, 4.3%). Most of them were Rh+ (386, 92.8%). The overall malaria prevalence was 4.1% (17/416). ABO blood group is significantly associated with malaria infection (P = 0.022). High rate of parasitemia was seen in blood group O donors (6.899, P = 0.003) compared to those with other ABO blood groups. Conclusion. Blood groups O and AB phenotypes are the most and the least ABO blood groups, respectively. There is significant association between ABO blood group and asymptomatic malaria parasitemia.

  6. SALMON SOFT ROE DNA ON BLOOD CELLS SECRETION OF CYTOKINES IN HEALTHY DONORS

    Directory of Open Access Journals (Sweden)

    L. N. Fedjanina

    2005-01-01

    Full Text Available Abstract. Salmon soft roe DNA influence on healthy donors blood cells secretion of early hemopoietic factors (IL-3, GM-CSF, TNFα as well as biologically active substance influence on cytokine balance of Тh1 and Тh2 responses (IFNγ, IL-10 in vitro was studied. It is established, that DNA has modulatory effect on secretion of all investigated cytokines - IL-3, GM-CSF, TNFα, INFγ and IL-10 by blood cells of healthy donors, increases their initially low concentration, reduces initially high and does not have essential influence at an average level of their secretion. Under action of DNA IFNγ level (stimulation index=3,3 increases more significantly than IL-10 level (stimulation index =1,9. Thus, salmon soft roe DNA possesses immunomodulatory properties.

  7. Adverse effects to transfusion with red donor blood cells are frequent

    DEFF Research Database (Denmark)

    Pommergaard, Hans-Christian; Nørgaard, Astrid; Burcharth, Jakob

    2014-01-01

    Adverse effects to transfusion with red donor blood cells are potentially life-threatening. Due to screening, transmission of infectious diseases has decreased; however, the risk is still present. Various immune reactions are common including simple allergic reactions as well as devastating...... conditions such as transfusion-related acute lung injury and circulatory overload in patients with heart disease. Knowledge of the clinical signs of transfusion-related complications is important for clinicians in order to provide the best possible treatment....

  8. CYP11B2 gene polymorphism among coronary heart disease patients and blood donors in Malaysia.

    Science.gov (United States)

    Normaznah, Y; Azizah, M R; Kuak, S H; Rosli, M A

    2015-04-01

    Various previous studies have reported the implication of CYP11B2 gene polymorphism in the pathophysiology of cardiovascular diseases. In particular, the -344T/C polymorphism, which is located at a putative binding site for the steroidogenic transcription factor (SF-1) has been associated with essential hypertension, left ventricular dilation and coronary heart disease. In the present study, we aim to determine the allele and genotype frequencies of the CYP11B2 gene in patients with clinical manifestation of coronary heart disease and confirmed by angiography and blood donors and to calculate the association of the gene polymorphism with CHD. A total of 79 DNA from patients with coronary heart disease admitted to the National Heart Institute and 84 healthy blood donors have been genotyped using polymerase chain reaction technique followed by restriction enzyme digestion (RFLP). Results of the study demonstrated that out of 79 for the patients, 40 were homozygous T, 10 were homozygous C and 29 were heterozygous TC. The frequencies of genotype TT, CC and TC for patients were 0.5, 0.13 and 0.36 respectively. The frequencies of allele T and C in patients were 0.68 and 0.31 respectively. While for the blood donors, 40 subjects were of homozygous T, 7 were homozygous C and 37 were heterozygous TC. The genotype frequencies for the TT, CC and TC were 0.47, 0.08 and 0.44 respectively. The frequency of the allele T was 0.69 and allele C was 0.3. Chi-Square analysis showed that there was no significant difference in the genotype and C allele frequencies between the CHD patients and the blood donors. Our study suggests that there is lack of association between -344T/C polymorphism of CYP11B2 gene and coronary heart disease.

  9. Immunity to diphtheria and tetanus among blood donors in Arak, central province of Iran.

    OpenAIRE

    Ali Eslamifar; Amitis Ramezani; Mohammad Banifazl; Masoomeh Sofian; Fatemeh-Alsadat Mahdaviani; Farhad Yaghmaie; Arezoo Aghakhani

    2014-01-01

    Background and Objectives Tetanus and diphtheria are vaccine-preventable, infectious diseases with significant morbidity and mortality. Immunization by the diphtheria and tetanus toxoid (DT) has been applied in Iran for almost 50 years. However, there are very few data about the rate of immunity to these diseases in the adult population. the humoral immunity to tetanus and diphtheria among blood donors in Arak city, central provice of Iran were investigated. Patients & Methods A total of 530 ...

  10. Demographic Correlates of Low Hemoglobin Deferral among Prospective Whole Blood Donors

    Science.gov (United States)

    Mast, Alan E.; Schlumpf, Karen S.; Wright, David J.; Custer, Brian; Spencer, Bryan; Murphy, Edward L.; Simon, Toby L.

    2010-01-01

    BACKGROUND Approximately 10% of attempted blood donations are not allowed because of low hemoglobin deferral. STUDY DESIGN AND METHODS Low hemoglobin deferrals were tracked in over 715,000 whole blood donors at six blood centers across the United States. A multivariable logistic regression model was developed to comprehensively assess demographic correlates for low hemoglobin deferral. RESULTS Demographic factors significantly associated with low hemoglobin deferral include female gender (11 times greater odds than males), increasing age in men (men over 80 have 29 times greater odds than men under 20); African American race (2–2.5 times greater odds than Caucasians); Hispanic ethnicity in women (1.29 times greater odds than Caucasian women) and weight in men (men under 124 pounds have 2.5 times greater odds than men over 200 pounds). Interestingly, increasing donation frequency is associated with decreased odds for low hemoglobin deferral (women with 1 donation in the previous 12 months have 2 times greater odds than those with 6 donations). CONCLUSIONS Low hemoglobin deferral is associated with female gender, older age, African-American race/ethnicity and lower body weight in men. An inverse association with donation frequency suggests a selection bias in favor of donors able to give more frequently. These data provide useful information that can be utilized to manage blood donors in order to limit low hemoglobin deferrals and assist in policy decisions such as changing the hemoglobin cut-off or permissible frequency of donation. They also generate hypotheses for new research of the causes of anemia in defined groups of donors. PMID:20412525

  11. Comparing prevalence of Iron Deficiency Anemia and Beta Thalassemia Trait in microcytic and non-microcytic blood donors: suggested algorithm for donor screening

    Directory of Open Access Journals (Sweden)

    Tiwari Aseem

    2009-01-01

    Full Text Available Background: The prevalence of microcytosis in donors and Iron Deficiency Anemia (IDA and Beta-Thalassemia trait (BTT in microcytic and non-microcytic donors has not been studied in India. The present study aims at finding the same. Materials and Methods: Initially 925 donor samples were evaluated on cell-counter. Of these, 50 were found to be microcytic. These were subjected to Ferritin and HbA2 determination. Subsequently, an additional 51, age-and-sex matched non-microcytic donor samples were selected to serve as controls. These were subjected to the same tests. Results: The prevalence of microcytosis was 5.4% (50/925. Among the microcytic donors, 52% were IDA, 36% BTT, 8% both, and 4% none. In case of non-microcytic donors 29.4% were IDA, 3.9% BTT, and 66.7% none. Conclusions: The study revealed a high prevalence of IDA and BTT in blood donors and a higher probability of finding these in the microcytic samples. This prompted authors to suggest an algorithm for screening of blood donors for IDA and BTT. The algorithm recommends doing an hemogram on all donor samples, routinely. Ferritin could be done only in microcytic samples. At levels lower than15 ng/ml, it is diagnosed as IDA, and therefore, HPLC is performed only for non-IDA samples with Ferritin levels higher than 15 ng/ml. By employing this algorithm, a substantial number of IDA and BTT could be diagnosed while keeping the number of Ferritin tests small and the number of HPLC tests even smaller and thus making it cost efficient.

  12. Serological Patterns and Molecular Characterization of Occult Hepatitis B Virus Infection among Blood Donors

    Science.gov (United States)

    Lin, Hong; Zhao, Hong; Tang, Xinyi; Hu, Wenjia; Jiang, Nizheng; Zhu, Shaowen; Huang, Chengyin

    2016-01-01

    Background Hepatitis B infections, characterized by the presence of a viral genome without detectable hepatitis B surface antigen (HBsAg; Occult hepatitis B infection [OBI]), have been reported recently. Objectives We performed serological and molecular characterization of OBI among blood donors at Jiangsu province blood center during years 2013 and 2014. Methods All donor samples were routinely screened by double enzyme-linked immunosorbent assay (ELISA) for hepatitis C virus (HCV), hepatitis B virus (HBV), human immunodeficiency virus (HIV), Treponema pallidum (TP), and alanine aminotransferase (ALT). Single-reactive, nonreactive, and ALT-elevated samples were pooled or resolved by nucleic acid testing (NAT). Seromarkers were examined in HBsAg-/DNA+ samples. After 1 to 12 months of follow up, seromarkers were screened again to verify OBI samples. Results We studied 157119 samples from blood donors. A total of 154397 ELISA nonreactive donor samples were identified, and HBV DNA was detected in 81 samples; no samples were positive for HIV or HCV RNA. Hepatitis B virus viral loads in most donors were less than 20 - 200 IU/mL. There was only one HBsAg-positive sample. Eighty HBsAg-/DNA+ samples were evaluated further. Of these samples, 85% (68/80) were reactive for anti-HBc and 36.2% (29/800) were reactive for anti-HBc and anti-HBs; 11.3% (9/80) did not have any detectable serological markers. Twenty-nine donors were followed up. One was HBsAg ELISA positive, and of six seronegative donors, all had anti-HBc and anti-HBs, but were negative for DNA. Samples were HBV genotypes B, C and D. Mutations in the S region of HBV DNA included S114T, G119R, P120S, T125M, C139Y, T140I, C147W, T148A, A159V/G, E164D, V168A, and R169C. Conclusions Overall, we found that OBI was rare, but that the prevalence of OBI was slightly higher in Jiangsu than in other areas of China. PMID:27882070

  13. Serologic survey on hantavirus in blood donors from the state of Santa Catarina, Brazil.

    Science.gov (United States)

    Cordova, Caio Maurício Mendes de; Figueiredo, Luiz Tadeu Moraes

    2014-01-01

    Emergent diseases such as Hantavirus Cardio-pulmonary Syndrome (HCPS) are able to create a significant impact on human populations due to their seriousness and high fatality rate. Santa Catarina, located in the South of Brazil, is the leading state for HCPS with 267 reported cases from 1999 to 2011. We present here a serological survey on hantavirus in blood donors from different cities of the state of Santa Catarina, with an IgG-ELISA using a recombinant nucleocapsid protein from Araraquara hantavirus as an antigen. In total, 314 donors from blood banks participated in the study, geographically covering the whole state. Among these, 14 individuals (4.4%) had antibodies to hantavirus: four of 50 (8% positivity) from Blumenau, four of 52 (7.6%) from Joinville, three of 50 (6%) from Florianópolis, two of 50 (4%) from Chapecó and one of 35 (2.8%) from Joaçaba. It is possible that hantaviruses are circulating across almost the whole state, with important epidemiological implications. Considering that the seropositive blood donors are healthy individuals, it is possible that hantaviruses may be causing unrecognized infections, which are either asymptomatic or clinically nonspecific, in addition to HCPS. It is also possible that more than one hantavirus type could be circulating in this region, causing mostly benign infections.

  14. Translation strategies, contradiction, and the theory of social representations: Why discussing needles may improve blood donor retention.

    Science.gov (United States)

    Moloney, Gail; Hayman, Jane; Gamble, Marguerite; Smith, Geoff; Hall, Rob

    2017-02-15

    Retaining blood donors is a cost-effective way of ensuring a safe blood supply, yet despite the plethora of research, only 5.1% of the eligible population in Australia donate blood and 40% of these do not make a second donation. We offer an alternative to traditional approaches by conceptualizing blood donation within social representations theory as socially derived symbolic knowledge with a specific focus on cognitive polyphasia and Guimelli's (1998) normative and functional dimensions. An online survey, completed by 703 residents from NSW Australia, comprised a blood donation word association task, Likert-style questions constructed from previous word association data and contextualized blood donation statements. Individual difference scaling analysis revealed all donor groups (including non-donors) associated blood donation with a few central, albeit contradictory ideas/beliefs. Exploratory factor analysis and confirmatory factor analysis performed on a split data set of the Likert-style items reiterated this finding. Interpreted through Guimelli's dichotomy, all donor groups were aware of these contradictory normative and functional ideas/beliefs but when explicitly asked, it was the functional aspect that differentiated the groups. We argue the key to retaining donors is understanding the interdependence between how blood donation is socially understood at the societal level of discourse and donor behaviour. Translational strategies for recruitment and retention are discussed.

  15. Automatic system for blood donors control with seropositive results in epidemiological tests.

    Directory of Open Access Journals (Sweden)

    Ariel Menéndez Barrios

    2008-04-01

    Full Text Available Background: Prevention of infectious diseases transmission through blood transfusion is one of the biggest challenges in the transfusion field of medicine. Objective: to design a computerized system for blood donors control with seropositive results in epidemiological tests. Methods: A system designed by the Provincial Blood Bank using Microsoft Access as a data base system and DELFHI 7 as associated language. It runs under Windows 2000 or any other superior system with 64 RAM and do not need special requirements of software or hardware. It includes the following data: Full name, age, sex, race, Id number, home address, number of the clinical record in the blood bank, date when the trial took place in each patient, kind of disease diagnosed, technical data of the trial such as: fluorescence, sectional level of the 1st trial as well as the repetition, name of the technician who performed it. Results: It permits to consult general data of donors as well as the disease diagnosed. It also permits to elaborate reports of these patients from any PC of the blood bank acceding with the security password of the program. Conclusions: It constitutes a beneficial tool which permits to improve the notification system established in the program of the web about sexually transmitted diseases from the Minister of Public Health in Cuba.

  16. Post-prandial rise of microvesicles in peripheral blood of healthy human donors.

    Science.gov (United States)

    Suštar, Vid; Bedina-Zavec, Apolonija; Stukelj, Roman; Frank, Mojca; Ogorevc, Eva; Janša, Rado; Mam, Keriya; Veranič, Peter; Kralj-Iglič, Veronika

    2011-03-21

    Microvesicles isolated from body fluids are membrane - enclosed fragments of cell interior which carry information on the status of the organism. It is yet unclear how metabolism affects the number and composition of microvesicles in isolates from the peripheral blood. To study the post - prandial effect on microvesicles in isolates from the peripheral blood of 21 healthy donors, in relation to blood cholesterol and blood glucose concentrations. The average number of microvesicles in the isolates increased 5 hours post - prandially by 52%; the increase was statistically significant (p = 0.01) with the power P = 0.68, while the average total blood cholesterol concentration, average low density lipoprotein cholesterol concentration (LDL-C) and average high density lipoprotein cholesterol concentration (HDL-C) all remained within 2% of their fasting values. We found an 11% increase in triglycerides (p = 0.12) and a 6% decrease in blood glucose (p microvesicles negatively correlated with the post - fasting total cholesterol concentration (r = - 0.46, p = 0.035) while the difference in the number of microvesicles in the isolates between post - prandial and post - fasting states negatively correlated with the respective difference in blood glucose concentration (r = - 0.39, p = 0.05). In a population of healthy human subjects the number of microvesicles in isolates from peripheral blood increased in the post - prandial state. The increase in the number of microvesicles was affected by the fasting concentration of cholesterol and correlated with the decrease in blood glucose.

  17. Seroprevalence and trends in transfusion transmitted infections among blood donors in a university hospital blood bank: a 5 year study.

    Science.gov (United States)

    Pallavi, P; Ganesh, C K; Jayashree, K; Manjunath, G V

    2011-03-01

    Blood is life. Transfusion of blood and blood components, as a specialized modality of patient management saves millions of lives worldwide each year and reduce morbidity. It is well known that blood transfusion is associated with a large number of complications, some are only trivial and others are potentially life threatening, demanding for meticulous pretransfusion testing and screening particularly for transfusion transmissible infections (TTI). These TTI are a threat to blood safety. The priority objective of BTS is thus to ensure safety, adequacy, accessibility and efficiency of blood supply at all levels. The objective of the present study was to assess the prevalence and trend of transfusion transmitted infections (TTI) among voluntary and replacement donors in the Department of Blood bank and transfusion Medicine of JSS College Hospital, a teaching hospital of Mysore during the period from 2004 to 2008. A retrospective review of donors record covering the period between 2004 and 2008 at the blood bank, JSS Hospital, Mysore was carried out. All samples were screened for HIV, HBsAg, HCV, syphilis and malaria. Of the 39,060, 25,303 (64.78%) were voluntary donors and the remaining 13,757 (35.22%) were replacement donors. The overall prevalence of HIV, HbsAg, HCV and syphilis were 0.44, 1.27, 0.23 and 0.28%, respectively. No blood donor tested showed positivity for malarial parasite. Majority were voluntary donors with male preponderance. In all the markers tested there was increased prevalence of TTI among the replacement donors as compared to voluntary donors. With the implementation of strict donor criteria and use of sensitive screening tests, it may be possible to reduce the incidence of TTI in the Indian scenario.

  18. The risk of transfusion-acquired hepatitis-C virus infection among blood donors in Port Harcourt: the question of blood safety in Nigeria.

    Science.gov (United States)

    Erhabor, O; Ejele, O A; Nwauche, C A

    2006-06-01

    This study was undertaken to establish the sero-epidemology of Hepatitis C Virus (HCV) antibodies among blood donors in Port Harcourt, Nigeria. One Thousand Five Hundred consecutive blood donors presenting to the blood transfusion unit of the University of Port Harcourt Teaching Hospital between January and April, 2003 comprising of 1481 males and 19 females were screened for hepatitis C antibodies using the commercially available Clinotech anti-HCV test strips. All initially positive samples were subsequently tested using a second-generation Trinity Biotec enzyme linked immunosorbent assay. HCV antibodies were detected in 7 (0.5%) of donors. Although statistically not significant, the overall sero prevalence of HCV antibodies was higher in males 7 (0.5%) compared to zero prevalence among females. (chi-squared = 1.94, p = 1.000). Commercial remunerated donors had a higher prevalence of anti-HCV anti-bodies 5 (0.8%) compared to family replacement donors (0.2%) (chi-squared = 1.25, p = 0.26). The highest infection rate occurred in the 18 - 27 years age group 7 (0.7%). This study shows a 0.5% prevalence of HCV antibodies among blood donors and describes their demographic characteristics. This calls for urgent implementation of a universal donor screening for HCV antibodies and setting up of a national blood transfusion service run on the basis of voluntary, non-remunerated low risk donors.

  19. Analysis of HCV genotypes from blood donors shows three new HCV type 6 subgroups exist in Myanmar.

    Directory of Open Access Journals (Sweden)

    Shinji T

    2004-06-01

    Full Text Available The prevalence of hepatitis C virus (HCV genotypes in Myanmar in comparison with the rest of Southeast Asia is not well known. Serum samples were obtained from 201 HCV antibody-positive volunteer blood donors in and around the Myanmar city of Yangon. Of these, the antibody titers of 101 samples were checked by serial dilution using HCV antibody PA test II and Terasaki microplate as a low-cost method. To compare antibody titers by this method and RNA identification, we also checked HCV-RNA using the Amplicor 2.0 test. Most high-titer groups were positive for HCV-RNA. Of the 201 samples, 110 were successfully polymerase chain reaction (PCR amplified. Among them, 35 (31.8% were of genotype 1, 52 (47.3% were of genotype 3, and 23 (20.9% were of type 6 variants, and phylogenetic analysis of these type 6 variants revealed that 3 new type 6 subgroups exist in Myanmar. We named the subgroups M6-1, M6-2, and M6-3. M6-1 and M6-2 were relatively close to types 8 and 9, respectively. M6-3, though only found in one sample, was a brand-new subgroup. These subtypes were not seen in Vietnam, where type 6 group variants are widely spread. These findings may be useful for analyzing how and when these subgroups were formed.

  20. The JAK2V617F tyrosine kinase mutation in blood donors with upper-limit haematocrit levels

    Science.gov (United States)

    Tagariello, Giuseppe; Di Gaetano, Rosanna; Sartori, Roberto; Zanotto, Daniela; Belvini, Donata; Radossi, Paolo; Risato, Renzo; Roveroni, Giovanni; Salviato, Roberta; Tassinari, Cristina; Toffano, Nunzio

    2009-01-01

    Background It is not rare to observe in blood donors a level of haematocrit (Hct) above or close to the highest normal limit. In the case of blood donors the diagnosis and clinical evaluation of this alteration may be complicated by regular blood donations that can mask an underlying disease such as polycythaemia vera. Recently a single acquired mutation in the Janus kinase 2 gene (JAK2) on chromosome 9 was identified and it was found that the incidence of this mutation was high in patients with polycythaemia vera. Material and Methods From the January 1, 2006 to December 31, 2006 all consecutive donors with a Hct above 50% if males (n=84) and 46% if females (n=19) underwent JAK2 mutation analysis. Seventy-nine donors (59 males and 20 females) whose Hct was normal at their last blood donation were randomly selected and used as controls. Results Among the group of blood donors with a high Hct, we identified one donor who was positive for the JAK2 mutation. This man had a Hct of 50.6% at his last donation, while his average Hct in the preceding year was 51.7%. The prevalence of the JAK2 mutation could be estimated to be 1%, 0.6% or 0.02% in the three different populations considered: donors with a Hct level above the upper limit of normal, all tested donors or the entire donor cohort attending our transfusion service, respectively. Conclusions The present study suggests that apparently healthy subjects with repeatedly high levels of Hct may have the acquired mutation in JAK2. Laboratory screening tests for JAK2 may be offered to blood donors at transfusion services with expertise in molecular genetics. PMID:19503632

  1. Selective Immunoglobulin A Deficiency in Iranian Blood Donors: Prevalence, Laboratory and Clinical Findings

    Directory of Open Access Journals (Sweden)

    Saghafi Shiva

    2008-09-01

    Full Text Available Selective deficiency of immunoglobulin A (IgA is the most frequent primary hypogammaglobulinemia. As some IgA-deficient patients have IgA antibodies in their plasma which may cause anaphylactic reactions, blood centers usually maintain a list of IgA-deficient blood donors to prepare compatible blood components. In this study we determined the incidence of selective IgA deficiency (SIgAD in normal adult Iranian population. 13022 normal Iranian blood donors were included in this study. The assay which we used was adapted to the manual pipetting system and ELISA reader was used for screening. Other classes of immunoglobulins (G, M, as well as secretory IgA and IgG subclasses were tested in IgA deficient cases by ELISA. SPSS was used for statistical analysis.Among 13022 studied cases, 11608 blood donors were males (89.14% and 1414 were females (10.86%. Their mean (±SD age and weight were 38.5±11 years and 82±12 Kg respectively. Twenty of the screened samples were found by means of ELISA to be IgA-deficient (less than 5mg/dl, (frequency; 1:651. The data could indicate a compensation for IgA deficiency by serum IgM in one of our IgA deficient cases (Patient 5. We observed a correlation between IgG3 and serum IgA in deficient cases (r=0.498, P=0.025. Our results indicate that in present study the prevalence of S IgA D is in agreement with data from other Caucasians populations (from 1:300 to 1:700. In conclusion, Selective IgA Deficiency could be almost asymptomatic in most cases in general population. Our study suggests that; due to high frequency of IgA deficiency in Iran, it seems necessary to measure IgA levels for every blood donor and blood recipient to find IgA deficient cases.

  2. Prevalence of Antibody to Hepatitis C Virus in Saudi Blood Donors

    Directory of Open Access Journals (Sweden)

    Bandar Al-Knawy

    1995-01-01

    Full Text Available The prevalence of antibodies to hepatitis C virus (anti-HCV was retrospectively determined using a second generation enzyme immunoassay in 3868 blood donors from the southern part of Saudi Arabia in an area with high prevalence of hepatitis B virus (HBV infection. Of 3354 Saudis, 48 (1.43% were seropositive for anti-HCV. A high prevalence (43 of 204, 21.08% of anti-HCV was observed among Egyptian donors compared with Saudis (1.43% and other nationalities (eight of 310, 2.58%. Furthermore, the prevalence of anti-HCV antibodies was observed to increase with age, peaking in the 25 to 34 year age group. From this and other studies conducted in different regions of Saudi Arabia, the prevalence of anti-HCV among Egyptian donors appears to range from 19.2 to 24.5%, and among Saudi donors appears to range from 1.00 to 1.7%, a rate similar to that reported from western countries; this latter rate does not seem to be influenced by the high prevalence of HBV infection in this region.

  3. Hepatitis C virus infection in blood donors from the state of Puebla, Mexico.

    Science.gov (United States)

    Sosa-Jurado, Francisca; Santos-López, Gerardo; Guzmán-Flores, Belinda; Ruiz-Conde, Julia I; Meléndez-Mena, Daniel; Vargas-Maldonado, Martín T; Martínez-Laguna, Ygnacio; Contreras-Mioni, Laura; Vallejo-Ruiz, Verónica; Reyes-Leyva, Julio

    2010-01-25

    Worldwide, 130 million persons are estimated to be infected with HCV. Puebla is the Mexican state with the highest mortality due to hepatic cirrhosis. Therefore, it is imperative to obtain epidemiological data on HCV infection in asymptomatic people of this region. The objective of present study was to analyze the prevalence of antibodies and genotypes of hepatitis C virus (HCV) in blood donors from Puebla, Mexico. The overall prevalence was 0.84% (515/61553). Distribution by region was: North, 0.86% (54/6270); Southeast, 1.04% (75/7197); Southwest, 0.93% (36/3852); and Central, 0.79% (350/44234). Ninety-six donors were enrolled for detection and genotyping of virus, from which 37 (38.5%) were HCV-RNA positive. Detected subtypes were: 1a (40.5%), 1b (27.0%), mixed 1a/1b (18.9%), undetermined genotype 1 (5.4%), 2a (2.7%), 2b (2.7%), and mixed 1a/2a (2.7%). All recovered donors with S/CO > 39 were HCV-RNA positive (11/11) and presented elevated ALT; in donors with S/CO Puebla is similar to other Mexican states. The most prevalent genotype is 1, of which subtype 1a is the most frequent.

  4. Hepatitis B and C prevalences among blood donors in the south region of Brazil

    Directory of Open Access Journals (Sweden)

    H. C. F. F. Vasconcelos

    1994-12-01

    Full Text Available The prevalence of hepatitis B and C infection has been determined in a seroepidemiological survey among blood donors from the south of Brazil (Florianópolis, State of Santa Catarina. These markers has also been correlated with the levels of alanine aminotransferase (ALT, a surrogate marker to prevent post-transfusion hepatitis. Sera from 5000 donors were randomly collected in the period of April to November 1991. The prevalences of HBsAg, anti-HBs and anti-HBc were respectively 0.78, 7.02 and 13.98. The anti-HCV prevalence after confirmation testing with line immunoassay (LIA, was 1.14. Normal values of ALT ( = 70 U/ml in 2.48. The positivity of anti-HCV antibodies increased with the elevation of ALT levels. This correlation was not observed in relation to HBsAg. There exists a diversity in the recognition of HCV epitopes among HCV positive donors. Via the confirmation test used, we could observe that 94.7 of donors recognize the structural core antigen. Besides that, we observed that 5.26 of the HCV reactive sera recognized only epitopes located in the NS4 and/or NS5 region, indicating the importance of these epitopes for the improvement of assays.

  5. Distribution of ABO and Rh types in voluntary Blood donors in Jharkhand area as a study conducted by RIMS, Ranchi.

    Science.gov (United States)

    Singh, Anu; Srivastava, Ramesh Kumar; Deogharia, Kabita S; Singh, Kranti Kumar

    2016-01-01

    This study was done to know the distribution and frequencies of blood groups among blood donors attending voluntary blood donation camps organized by the Rajendra Institute of Medical Sciences (RIMS), Ranchi, Jharkhand so that demand and supply ratio of the four blood groups can be maintained so that no patient dies due to lack of a particular blood group. Up till now about 400 red cells antigen have been identified. The majority follow Mendelian inheritance. The ABO and Rhesus (Rh) blood group system are most important for blood transfusion purposes, parental testing, legal medicine, and in population genetic study. This study was conducted to determine and compare the frequency and distribution of ABO and Rh blood groups among voluntary blood donors attending blood donation camps in Jharkhand organized by RIMS. The aim is to know the demand and supply ratio of a particular blood group in light of their distribution in the society so that no patient dies due to the deficient supply of blood. It is a retrospective study carried out at blood bank. Materials and Methods: A study was conducted at Blood bank, RIMS, Ranchi, Jharkhand, over a period of 4 years from January 1, 2012, to December 31, 2015. Blood group of the blood donors was determined by commercially available standard monoclonal antisera by test tube agglutination technique accompanied by reverse grouping. Out of 20,455 subjects, 18,717 (91.73%) were male and 1738 (8.27%) were female subjects. The ABO blood group present was B (35.15%) followed by O (34.73%), A (22.09%), and AB (8.03%) in blood donors while in Rh system, (96.46%) donors were Rh +ve and (3.54%) donors were Rh -ve. The study has a significant implication regarding the inventory management of blood bank and transfusion services for the indoor patients of RIMS and for emergency supply to other hospitals of Jharkhand in dire need of blood. The knowledge of distribution of blood group is very important for blood banks and transfusion services

  6. Hydroxyl-platelet-activating factor exists in blood of healthy volunteers and periodontal patients

    Directory of Open Access Journals (Sweden)

    Smaragdi Antonopoulou

    2003-01-01

    Full Text Available Periodontal diseases are localized chronic inflammatory conditions of the gingival and underlying bone and connective tissue. Platelet-activating factor (PAF, a potent inflammatory phospholipid mediator that has been previously detected in elevated levels in inflamed gingival tissues, in gingival crevicular fluid and in saliva, is implicated in periodontal disease. Our results from previous studies showed that the biologically active phospholipid detected in gingival crevicular fluid is a hydroxyl-PAF analogue. In this study, hydroxyl-PAF analogue was detected for the first time in human blood derived from patients with chronic periodontitis as well as from periodontally healthy volunteers. The hydroxyl-PAF analogue was purified by high-performance liquid chromatography, detected by biological assays and identified by electrospray analysis. In addition, the quantitative determination of PAF and hydroxyl-PAF analogue (expressed as PAF-like activity showed a statistically significant increase in the ratio of hydroxyl-PAF analogue levels to PAF levels in periodontal patients, suggesting that this bioactive lipid may play a role in oral inflammation.

  7. Phosphatidylserine exposure on stored red blood cells as a parameter for donor-dependent variation in product quality

    NARCIS (Netherlands)

    Dinkla, S.; Peppelman, M.; Raadt, J. van der; Atsma, F.; Novotny, V.M.J.; Kraaij, M.G.J. van; Joosten, I.; Bosman, G.J.C.G.M.

    2014-01-01

    BACKGROUND: Exposure of phosphatidylserine on the outside of red blood cells contributes to recognition and removal of old and damaged cells. The fraction of phosphatidylserine-exposing red blood cells varies between donors, and increases in red blood cell concentrates during storage. The susceptibi

  8. Hepatitis E virus IgG seroprevalence in HIV patients and blood donors, west-central Poland

    Directory of Open Access Journals (Sweden)

    Maciej Bura

    2017-08-01

    Conclusions: Wielkopolska Region in west-central Poland is an area hyperendemic for HEV infection. In this part of Poland, the exposure of HIV-positive persons to this virus is not greater than that of healthy blood donors.

  9. Skin Recurrence of Transformed Mycosis Fungoides Postumbilical Cord Blood Transplant despite Complete Donor Chimerism

    Directory of Open Access Journals (Sweden)

    Rahul Pawar

    2014-01-01

    Full Text Available Background. Allogeneic stem cell transplant is the treatment of choice for systemic cutaneous T-cell lymphoma (CTCL which provides graft-versus-lymphoma effect. Herein we discuss a case of recurrence of CTCL skin lesions after cord blood transplant in a patient who continued to have 100% donor chimerism in bone marrow. Case Presentation. A 48-year-old female with history of mycosis fungoides (MF presented with biopsy proven large cell transformation of MF. PET scan revealed multiple adenopathy in abdomen and chest suspicious for lymphoma and skin biopsy showed large cell transformation. She was treated with multiple cycles of chemotherapy. Posttherapy PET scan showed resolution of lymphadenopathy. Later she underwent ablative preparative regimen followed by single cord blood transplant. Bone marrow chimerism studies at day +60 after transplant showed 100% donor cells without presence of lymphoma. However 5 months after transplant she had recurrence of MF with the same genotype as prior skin lesion. Bone marrow chimerism study continued to show 100% donor cells. Conclusion. A differential graft-versus-lymphoma effect in our case prevented lymphoma recurrence systemically but failed to do so in skin. We hypothesize that this response may be due to presence of other factors in the bone marrow and lymph node microenvironments preventing recurrence in these sites.

  10. [Blood donation: a marketing perspective].

    Science.gov (United States)

    Ludwig, Silvia Terra; Rodrigues, Alziro César de Morais

    2005-01-01

    This paper emphasizes how marketing can make a difference in repeat donations by volunteer blood donors, since the greatest challenge for health institutions is to maintain and increase blood donation. In this context, understanding volunteer donors' motivations is highly important, and the studies reported here demonstrate that several variables are relevant to blood donation. The huge number of patients in need of blood transfusions and the lack of sufficient blood and blood products justify the interest in this study, considering both donors' and blood banks' perspectives. Moreover, recognition of the importance of actions and orientation for donors is fundamental for developing a marketing strategy. It is thus relevant for health institutions to identify donors' actual needs and wishes.

  11. Seroepidemiology of Toxoplasma infection in blood donors in Jahrom District, Southern Iran

    Institute of Scientific and Technical Information of China (English)

    Mohammad Hassan Davami; Morteza Pourahmad; Rasoul Baharlou; Abdolreza Sotoodeh Jahromi; Abbass Ahmadi Vasmejani; Kavous Solhjoo; Hamid Reza Fallah; Mohsen Kalantari

    2015-01-01

    Objective: To identify the anti-Toxoplasma antibodies from blood donors who referred to blood transfusion bases of Jahrom County, using ELISA method. Methods: Based on the prevalence and characteristics method, 400 serum samples were collected from blood donors referred to Jahrom blood transfusion bases, Southern Iran, during 2010–2011, designed at testing by ELISA. IgM and IgG antibodies against Toxoplasma gondii were tested using ELISA kits (Dia-Pro) on serums. The data were analysed by SPSS 19 software. Results: Review of 400 cases, 54 of them were IgG positive for parasites (13.5%) and 346 of those with negative IgG (86.5%). In IgM examination, 1.75%of them have been positive IgM (7 cases) and 98.25% of them were IgM negative (393 cases). By comparing the different group ages, 40–50 year age group had the highest prevalence of IgG positive (17.9%) and the age group of 30–40 years had the highest incidence of IgM negative (2.5%). Conclusions: Due to the serological infection rate of toxoplasmosis obtained from this study, toxoplasmosis should be considered as a significant transfusion risk factor in Jahrom and also in any region with similar situations.

  12. Seroepidemiology of Toxoplasma infection in blood donors in Jahrom District,Southern Iran

    Institute of Scientific and Technical Information of China (English)

    Mohammad; Hassan; Davami; Morteza; Pourahmad; Rasoul; Baharlou; Abdolreza; Sotoodeh; Jahromi; Abbass; Ahmadi; Vasmejani; Kavous; Solhjoo; Hamid; Reza; Fallah; Mohsen; Kalantari

    2015-01-01

    Objective:To identify the anti-Toxoplasma antibodies from blood donors who referred to blood transfusion bases of Jahrom County,using ELISA method.Methods:Based on the prevalence and characteristics method,400 serum samples were collected from blood donors referred to Jahrom blood transfusion bases,Southern Iran,during 2010–2011,designed at testing by ELISA.Ig M and Ig G antibodies against Toxoplasma gondii were tested using ELISA kits(Dia-Pro)on serums.The data were analysed by SPSS 19 software.Results:Review of 400 cases,54 of them were Ig G positive for parasites(13.5%)and 346of those with negative Ig G(86.5%).In Ig M examination,1.75%of them have been positive Ig M(7 cases)and 98.25%of them were Ig M negative(393 cases).By comparing the different group ages,40–50 year age group had the highest prevalence of Ig G positive(17.9%)and the age group of 30–40 years had the highest incidence of Ig M negative(2.5%).Conclusions:Due to the serological infection rate of toxoplasmosis obtained from this study,toxoplasmosis should be considered as a significant transfusion risk factor in Jahrom and also in any region with similar situations.

  13. Occult Hepatitis B Virus Infection in Nigerian Blood Donors and Hepatitis B Virus Transmission Risks.

    Directory of Open Access Journals (Sweden)

    Opaleye O Oluyinka

    Full Text Available Occult hepatitis B virus infection (OBI characterized by the absence of detectable HBsAg remains a potential threat in blood safety. We investigated the actual prevalence, viral factors and genotype of OBI infections in Nigerian blood donors.Serum collected from two blood banks were reconfirmed as HBsAg seronegative by ELISA. Forty HBsAg positive samples were employed as controls. HBV-DNA was amplified from all donors and viral loads were determined using quantitative real-time PCR. Antibodies to the HBV core, surface and HBe antigen (anti-HBc,anti-HBs,HBeAg were measured. The PreS/S and PreC/C regions of the HBV genome were sequenced.Of the 429 blood donors, 72(17% were confirmed as OBI by DNA detection in different reference labs and excluded the concern of possible contamination. Of the 72 OBI samples, 48(67% were positive for anti-HBc, 25(35% positive for anti-HBs, and 2(3% positive for HBeAg. Of the 72 OBI samples, 31(43% were seropositive for either anti-HBc, anti-HBs or HBeAg, 21 (30% positive for both anti-HBc and anti-HBs,one positive for both anti-HBc and HBeAg. None of the OBI samples were positive for all three serological markers. The viral load was <50copies/ml in the OBI samples and genotype E was predominant. The L217R polymorphism in the reverse transcriptase domain of the HBV polymerase gene was observed significantly higher in OBI compared with HBsAg positive individuals (P<0.0001.High incidence of OBI is relevant in high endemic areas worldwide and is a general burden in blood safety. This study signifies the high prevalence of OBI and proposes blood donor samples in Nigeria should be pre-tested for OBI by nucleic acid testing (NAT and/or anti-HBc prior to transfusion to minimize the HBV infection risk.

  14. Seroprevalence trends of transfusion transmitted infections among blood donors in a tertiary care hospital of Himachal Pradesh, India

    Directory of Open Access Journals (Sweden)

    Amit Sachdeva

    2016-11-01

    Results: A total of 39,083 blood donors of both sexes attended the blood bank during this period. Overall, HBC, HIV, syphilis and malaria rate for blood donors was found to be 0.45%. 0.16%, 0.08%, 0.07% and 0.003% respectively. There is a downward trend in sereoprevalence of all screened TTIs namely HBV, HCV, HIV and syphilis and malaria from 2008-2011. Conclusions: The study exhibits that over a period of years there is rise in voluntary blood donations which is heartening and encouraging. Trend analysis for prevalence TTIs among blood donors has shown a decreasing trend. It is recommended that continual quality assured screening of donated blood should be carried out as per the prescribed norms to deal with acquired TTIs. [Int J Res Med Sci 2016; 4(11.000: 5002-5006

  15. Evolutionary analysis of HBV "S" antigen genetic diversity in Iranian blood donors: a nationwide study.

    Science.gov (United States)

    Pourkarim, Mahmoud Reza; Sharifi, Zohre; Soleimani, Ali; Amini-Bavil-Olyaee, Samad; Elsadek Fakhr, Ahmed; Sijmons, Steven; Vercauteren, Jurgen; Karimi, Gharib; Lemey, Philippe; Maes, Piet; Alavian, Seyed Moayed; Van Ranst, Marc

    2014-01-01

    The genetic diversity of the HBV S gene has a significant impact on the prophylaxis and treatment of hepatitis B infection. The effect of selective pressure on this genetic alteration has not yet been studied in Iranian blood donors. To explore HBV evolution and to analyze the effects and patterns of hepatitis B surface antigen (HBsAg) mutations on blood screening assays, 358 Iranian blood donors diagnosed as asymptomatic HBV carriers were enrolled in this nationwide study. Large S and partial S genes were amplified and sequenced. HBV (sub) genotypes and synonymous and nonsynonymous mutations were investigated. The impact of naturally occurring mutations on HBsAg ELISA results was explored. Phylogenetic analyses revealed that isolated strains were of genotype D. The dominant subgenotype/subtype was D1/ayw2. Deletions and naturally occurring stop codons in the pre-S1 and major hydrophilic region (MHR) were identified. In total, 32.8% of the studied strains harbored 195 single or multiple mutations in the MHR, the majority of which were located at the first loop of the "a determinant" domain. The ayw2 subtype showed a significant effect on the ELISA signal/cut-off value and carried fewer mutations in the MHR. Nonsynonymous/synonymous substitution value indicated that negative selection was the dominant evolutionary force in the HBV S gene. This nationwide study revealed that mutation frequency of HBsAg among Iranian blood donors was much higher than previous reports from the different local regions. These findings regarding the significant differences in reactivity of ELISA among different subtypes of HBV and its correlation with the number of mutations at the MHR will be valuable to public health authorities.

  16. An analysis of first-time blood donors return behaviour using regression models.

    Science.gov (United States)

    Kheiri, S; Alibeigi, Z

    2015-08-01

    Blood products have a vital role in saving many patients' lives. The aim of this study was to analyse blood donor return behaviour. Using a cross-sectional follow-up design of 5-year duration, 864 first-time donors who had donated blood were selected using a systematic sampling. The behaviours of donors via three response variables, return to donation, frequency of return to donation and the time interval between donations, were analysed based on logistic regression, negative binomial regression and Cox's shared frailty model for recurrent events respectively. Successful return to donation rated at 49·1% and the deferral rate was 13·3%. There was a significant reverse relationship between the frequency of return to donation and the time interval between donations. Sex, body weight and job had an effect on return to donation; weight and frequency of donation during the first year had a direct effect on the total frequency of donations. Age, weight and job had a significant effect on the time intervals between donations. Aging decreases the chances of return to donation and increases the time interval between donations. Body weight affects the three response variables, i.e. the higher the weight, the more the chances of return to donation and the shorter the time interval between donations. There is a positive correlation between the frequency of donations in the first year and the total number of return to donations. Also, the shorter the time interval between donations is, the higher the frequency of donations. © 2015 British Blood Transfusion Society.

  17. Hepatitis E virus antibodies among patients with hemophilia, blood donors, and hepatitis patients.

    Science.gov (United States)

    Zaaijer, H L; Mauser-Bunschoten, E P; ten Veen, J H; Kapprell, H P; Kok, M; van den Berg, H M; Lelie, P N

    1995-07-01

    The presence of antibodies to hepatitis E virus (HEV) was studied among hemophiliacs, blood donors, and hepatitis patients. Four of 296 (1.4%) hemophiliacs and 5 of 1,275 (0.4%) donors were confirmed as positive for HEV antibodies (difference was not significant: P = 0.07). Parenteral transmission of HEV to hemophiliacs was thus rare or nonexistent. Seven of 187 hepatitis patients were found with HEV antibodies (IgG and IgM). Six persons fell ill shortly after arriving from HEV-endemic countries. The seventh patient, without a history of travel, represents a case of nontropical hepatitis E. Consequently, hepatitis E should be considered in patients suffering from acute non-ABC hepatitis, even in industrialized countries.

  18. Transfusion transmittable infections - Seroprevalence among blood donors in a tertiary care hospital of Delhi

    Directory of Open Access Journals (Sweden)

    Sangeeta Pathak

    2013-01-01

    Full Text Available Context: Transfusion transmittable infections (TTI continue to be a major threat to safe transfusion practices. Blood is one of the major sources of transmission of infectious diseases viz. human immunodeficiency virus (HIV, hepatitis B virus (HBV, hepatitis C virus (HCV, syphilis, malaria, and many other infections in India. Screening assays for the infectious diseases with excellent sensitivity and specificity helps to enhance the safety of the blood transfusions reducing the diagnostic window period as much as possible. Aims: The present study was designed to determine the seroprevalence of TTIs viz., HIV, HCV, and HBV, among the blood donors in Max Super Specialty Hospital, New Delhi, India based on dual testing strategy using high sensitive screening assays such as enhanced chemiluminescence assay and nucleic acid testing (NAT. Materials and Methods: A total of 41207 blood units collected from the donors (both voluntary and replacement donors were screened for the TTI s, viz., anti HIV 1 and 2 antibody, anti HCV antibody, anti HBcore antibody, and HBsAg by enhanced chemiluminescence assay on VITROS ® ECiQ immunodiagnostics system. NAT was performed using Roche Cobas ® TaqScreen MPX assay, which can detect simultaneously HIV 1 (groups M and O, HIV-2, HCV, and HBV on Roche Cobas ® s201 system. Results: The seroprevalence of HIV, HBsAg, anti HBcore antibody, and HCV based on enhanced chemiluminescence assay was found to be 0.25, 0.2, 7.06, and 0.7%, respectively. A total number of 6587 samples from July 2010 to December 2010 were tested on NAT, of which 3 samples were reactive for HBV in NAT; this was missed by enhanced chemiluminescence assay. Conclusions: Based on the seroprevalence study of infectious diseases viz., HIV, HBV, and HCV, we conclude that screening of blood and blood components by dual testing strategy using high sensitivity serological assay like enhanced chemiluminescence technology and NAT helps in detecting the

  19. Plasmodium genome in blood donors at risk for malaria after several years of residence in Italy.

    Science.gov (United States)

    Assennato, Sonny Michael; Berzuini, Alessandra; Foglieni, Barbara; Spreafico, Marta; Allain, Jean-Pierre; Prati, Daniele

    2014-10-01

    At present, the main risk of transfusion-transmitted malaria (TTM) in nonendemic countries is chronic, asymptomatic immigrants from malaria-endemic areas. Semi-immune donors may carry undetected parasitemia. This study examines Plasmodium infection in at-risk blood donors in Northern Italy. Plasma samples from 97 candidate donors and 80 controls were tested for malarial antibodies using a commercial enzyme immunoassay. The conserved 18S rRNA and the mitochondrial genes of Plasmodium were amplified to detect and quantify parasite genomes (copies/mL). Plasmodium species were identified with a species-specific nested polymerase chain reaction. Parasitemic samples were further tested by amplification of polymorphic repetitive regions in MSP-1 Block 2, MSP-2 Block 3, and glutamate-rich protein (GLURP) confirmed by sequencing. Three of 83 seropositive (3.6%) and one of 14 seronegative at-risk candidate donors carried Plasmodium genome (4 × 10(3) -8.5 × 10(4) copies/mL): two P. falciparum, one P. malariae (seronegative sample), and one coinfection with P. malariae and P. ovale. Alleles of MSP-1 (MAD20 and K1), MSP-2 (3D7 and FC27), and GLURP were amplified from Sample 261. In Sample 282 only one allele in MSP-2 (FC27) and GLURP was amplified. No alleles were detected in Samples 283 and 331. Immigrants from endemic countries might carry infectious Plasmodium after 2 to 5 years of continuous residence in Italy. Serologic screening may miss donors carrying P. malariae. Permanent exclusion or screening for both antibodies and genome are needed to prevent TTM. © 2014 AABB.

  20. Prospective evaluation of 2% (w/v alcoholic chlorhexidine gluconate as an antiseptic agent for blood donor arm preparation

    Directory of Open Access Journals (Sweden)

    Sweta Shah

    2014-01-01

    Full Text Available Aim: A prospective study was undertaken to evaluate the use of 2% (w/v alcoholic chlorhexidine gluconate (2% AlcCHG in donor arm preparation, to monitor the contamination rate of blood products after the collection and to find incidence of transfusion associated bacteremia. Settings and Design: Optimal skin antisepsis of the phlebotomy site is essential to minimize the risk of contamination. Food and Drug Administration (FDA in India has recommended antisepsis with three-step regimen of spirit-10% povidone iodine-spirit for donor arm antisepsis, but not with chlorhexidine, which is recommended by many other authors. Material and Methods: A total of 795 donors were studied from July 2011 to January 2012. Spirit-10% povidone iodine-spirit was used for 398 donors and 2% AlcCHG was used for 397 donors with the two-step method for arm antisepsis. Swabs were collected before and after use of antiseptic agents for all the donors. All the blood products collected from donors with growth in post-antisepsis swabs were cultured. A total of 123 various blood products were cultured irrespective of the method and result of antisepsis was observed. A total of seven patients had mild transfusion reaction. The transfused blood products, blood and urine specimen of the patients who had transfusion reaction were also cultured. Results: Seven donors out of 398 donors had growth in post-antisepsis swab with spirit-10% povidone iodine-spirit protocol and three donors out of 397 donors had growth in post-antisepsis swab with 2% AlcCHG protocol. All blood products collected from donors who had growth in post-antisepsis swabs when cultured had no growth. There was no contamination of blood products. Conclusions: Two percent (w/v alcoholic chlorhexidine gluconate with two-step protocol can be used as an antiseptic agent for donor arm preparation without considerable cost difference. It is at par with spirit 10% povidone iodine spirit protocol as suggested by FDA in India

  1. Effect of adrenergic agonists on coronary blood flow: a laboratory study in healthy volunteers.

    Science.gov (United States)

    Vargas Pelaez, Alvaro F; Gao, Zhaohui; Ahmad, Tariq A; Leuenberger, Urs A; Proctor, David N; Maman, Stephan R; Muller, Matthew D

    2016-05-01

    Myocardial oxygen supply and demand mismatch is fundamental to the pathophysiology of ischemia and infarction. The sympathetic nervous system, through α-adrenergic receptors and β-adrenergic receptors, influences both myocardial oxygen supply and demand. In animal models, mechanistic studies have established that adrenergic receptors contribute to coronary vascular tone. The purpose of this laboratory study was to noninvasively quantify coronary responses to adrenergic receptor stimulation in humans. Fourteen healthy volunteers (11 men and 3 women) performed isometric handgrip exercise to fatigue followed by intravenous infusion of isoproterenol. A subset of individuals also received infusions of phenylephrine (n = 6), terbutaline (n = 10), and epinephrine (n = 4); all dosages were based on fat-free mass and were infused slowly to achieve steady-state. The left anterior descending coronary artery was visualized using Doppler echocardiography. Beat-by-beat heart rate (HR), blood pressure (BP), peak diastolic coronary velocity (CBVpeak), and coronary velocity time integral were calculated. Data are presented as M ± SD Isometric handgrip elicited significant increases in BP, HR, and CBVpeak (from 23.3 ± 5.3 to 34.5 ± 9.9 cm/sec). Isoproterenol raised HR and CBVpeak (from 22.6 ± 4.8 to 43.9 ± 12.4 cm/sec). Terbutaline and epinephrine evoked coronary hyperemia whereas phenylephrine did not significantly alter CBVpeak. Different indices of coronary hyperemia (changes in CBVpeak and velocity time integral) were significantly correlated (R = 0.803). The current data indicate that coronary hyperemia occurs in healthy humans in response to isometric handgrip exercise and low-dose, steady-state infusions of isoproterenol, terbutaline, and epinephrine. The contribution of β1 versus β2 receptors to coronary hyperemia remains to be determined. In this echocardiographic study, we demonstrate that coronary blood flow increases when

  2. [Single-donor (apheresis) platelets and pooled whole-blood-derived platelets--significance and assessment of both blood products].

    Science.gov (United States)

    Hitzler, Walter E

    2014-01-01

    The transfusion efficacy of ATK, which contain fully functional platelets, is beyond all doubt. The equivalence of ATK and PTK has been subject of many studies. Some of those studies show the superiority of ATK's, while others do not, but there have been no studies that demonstrated a superiority of PTK's. The superiority of platelets stored in plasma and in third generation additive solution was demonstrated in clinical studies; therefore, it cannot be said that all the platelet concentrates on the German market are equivalent in efficacy. Of decisive importance, above all, is the risk of transfusion-transmitted infections with known pathogens, or those not yet discovered. This risk is different for ATK compared to PTK. Taking this difference in risk and the difference in donor exposure of transfused patients into account, it can definitely be said that ATK and PTK are not equivalent. In 2012, the Robert-Koch-Institute (RKI) published a mathematical risk model for different platelet concentrates and assessed the risk of transmitting known pathogens such as HIV, HCV, and HBV. The risk was higher for PTK compared to ATK. The relative risks for PTK derived from 4BCs were 2.2 (95%--CI: 2.1-2.4) for HIV, 2.7 (95%--CI: 2.5-3.0) for HCV, and 2.2 (95%--CI: 2.8-3.7) for HBV. At the present time, these are the relative risks of transfusion-transmitted infections with the traditional pathogens for PTK compared to ATK. In addition to the RKI assessed risks, there is the theoretical risk of a new, unknown agent, transmitted through blood exposure. The magnitude of this risk is hardly predictable for PTK. The experience gathered so far, especially in the last three decades, with the emergence of HIV, prions, and West Nil virus, shows that the biological nature of a next transfusion-transmissible infectious agent cannot be predictable. This agent, if we think at a conventional sexually transmissible agent with nucleic acid and long latent period, would spread first in areas with

  3. Distribution of ABO and rhesus (D blood group antigens among blood donors at a tertiary care teaching hospital blood bank in south India

    Directory of Open Access Journals (Sweden)

    Suresh B

    2015-04-01

    Full Text Available Background: The ABO and Rhesus (Rh blood group systems are important for transfusion of blood and its components, organ transplantation, genetic studies and in medico-legal issues. Despite the long list of several other blood groups discovered so far, the knowledge and distribution of ABO and Rh-D blood group are essential for effective management of blood bank inventory. Methods: We retrospectively studied the distribution of ABO and Rh blood group antigens in donors presenting to our tertiary care teaching hospital blood bank in south India during the period January 2007 to August 2014. Blood group was determined by commercially available standard monoclonal antisera by test tube agglutination technique. Results: A total of 49,110 donor samples were tested during the study period for ABO grouping and Rh-D typing. Out of these 96.9% were males. The frequency of O, B, A, AB and Bombay blood groups were 41.7%, 32.2% 20%, 6.1% and 0.03% respectively. Rh (D positive and negative blood groups were seen in 92.8% and 7.2% respectively. The allele frequencies of the I A , IB and IO alleles were 0.1398, 0.2148 and 0.6454 respectively. In case of Rh-D group, the calculated gene frequencies for ID and Id were 0.7321 and 0.2679 respectively. Conclusion: Knowledge of blood group systems as documented in the present study helps in efficient management of blood bank and transfusion services in emergencies.

  4. Phenotype frequencies of blood group systems (Rh, Kell, Kidd, Duffy, MNS, P, Lewis, and Lutheran in blood donors of south Gujarat, India

    Directory of Open Access Journals (Sweden)

    Manoj A Kahar

    2014-01-01

    Full Text Available Background: This is the first study on phenotype frequencies of various blood group systems in blood donors of south Gujarat, India using conventional tube technique. Material and Methods: A total of 115 "O" blood group donors from three different blood banks of south Gujarat were typed for D, C, c, E, e, K, Jk a , Le a , Le b , P 1 , M, and N antigens using monoclonal antisera and k, Kp a , Kp b , Fy a ,Fy b , Jk b , S,s, Lu a , and Lu b antigens were typed using polyclonal antisera employing Indirect Antiglobulin Test. Antigens and phenotype frequencies were expressed as percentages. Results: From the 115 blood donor samples used for extended antigen typing in the Rh system, e antigen was found in 100% donors, followed by D [84.35%], C [81.74%], c [56.32%], and E [21.74%] with DCe/DCe (R 1 R 1 , 40.87% as the most common phenotype. k was found to be positive in 100% of donors and no K+k- phenotype was found in Kell system. For Kidd and Duffy blood group system, Jk(a+b+ and Fy(a-b- were the most common phenotypes with frequency of 52.17% and 48.69%, respectively. In the MNS system, 39.13% donors were typed as M+N+, 37.39% as M+N-, and 23.48% as M-N+. S+s+ was found in 24.35% of donors, S+s- in 8.69%, and S-s+ as the commonest amongst donors with 66.96%. No Lu(a+b+ or Lu(a+b- phenotypes were detected in 115 donors typed for Lutheran antigens. A rare Lu(a-b- phenotype was found in 2.61% donors. Conclusion: Data base for antigen frequency of various blood group systems in local donors help provide antigen negative compatible blood units to patients with multiple antibodies in order to formulate in-house red cells for antibody detection and identification and for preparing donor registry for rare blood groups.

  5. Novel genotype of Ehrlichia canis detected in samples of human blood bank donors in Costa Rica.

    Science.gov (United States)

    Bouza-Mora, Laura; Dolz, Gaby; Solórzano-Morales, Antony; Romero-Zuñiga, Juan José; Salazar-Sánchez, Lizbeth; Labruna, Marcelo B; Aguiar, Daniel M

    2017-01-01

    This study focuses on the detection and identification of DNA and antibodies to Ehrlichia spp. in samples of blood bank donors in Costa Rica using molecular and serological techniques. Presence of Ehrlichia canis was determined in 10 (3.6%) out of 280 blood samples using polymerase chain reaction (PCR) targeting the ehrlichial dsb conserved gene. Analysis of the ehrlichial trp36 polymorphic gene in these 10 samples revealed substantial polymorphism among the E. canis genotypes, including divergent tandem repeat sequences. Nucleotide sequences of dsb and trp36 amplicons revealed a novel genotype of E. canis in blood bank donors from Costa Rica. Indirect immunofluorescence assay (IFA) detected antibodies in 35 (35%) of 100 serum samples evaluated. Thirty samples showed low endpoint titers (64-256) to E. canis, whereas five sera yielded high endpoint titers (1024-8192); these five samples were also E. canis-PCR positive. These findings represent the first report of the presence of E. canis in humans in Central America.

  6. Changes in risk factors of HBsAg positive blood donors in Zahedan, Iran

    Directory of Open Access Journals (Sweden)

    Roya Alavi-Naini

    2011-07-01

    Full Text Available Background: Hepatitis B viral infection is a great global problem. According to high prevalence of infection in some parts of Iran, identifying etiologies and risk factors of the disease is an important issue in controlling infection in the community. The objective of this study is to compare the risk factors of HBsAg positive blood donors during a 10 year-period time-course.Materials and Method: We conducted a retrospective study in 1200 HBsAg positive blood donors referring to blood bank center in Zahedan during the years 1378, 1383 and 1387. Demographic variables and several risk factors were evaluated and compared during those years.Results: Our findings revealed significant statistical changes in age and education. Age was decreased and the disease was increased in illiterate cases during the study years. Risk factors that showed significant increasing changes during that time-period were history of dental procedures, surgery, hospitalization, endoscopy, positive family history for HBsAg, addiction to non-injectable drugs, living with positive HBsAg partners and intra-venous drug users. Conclusion: Decreasing the mean age of the patients during those years reflects increasing high risk behaviors among young people. Neonatal and family vaccination and screening family members, especially mothers could prevent this devastating disease. Following hygienic rules in the hospital is another important preventive measure. Premarital screening especially in high risk individuals is an effective preventive way to decrease the disease in the society.

  7. Characterization of rubella seronegative females in the Zambian blood donor community

    Directory of Open Access Journals (Sweden)

    Mazyanga Lucy Mazaba

    2015-04-01

    Full Text Available Rubella is an acute, contagious viral infection caused by a teratogenic enveloped single-stranded RNA virus, rubella virus, a member of the togaviridae family. Though causing generally mild infections in children and adults, it is a disease of public health importance in pregnant women causing major problems including abortions, miscarriages and Congenital Rubella Syndrome in more than twenty per cent of the susceptible population. This study was carried out to determine the characteristics associated with rubella seronegativity amongst female blood donors in Zambia. Rubella-specific IgG antibody levels were measured in the blood serum. Proportions were compared using the Chi-squared test at the 5% significance level, and magnitudes of associations were determined using the odds ratio and its 95% confidence interval. Of the 124 female blood donors tested for rubella IgG 46.0% were aged less than 20 years. Overall, 66.7% of the participants had never been married. More than half (62.1% of the participants resided in rural areas of the country. Of the 114 participants with recorded level of education, 50.1% had at–least completed secondary school. Out of 43 participants with recorded current employment status, 44.2% were not working for pay. A total of 10 (8.1% participants were sero-negative to rubella IgG antibodies. No factors were associated with seronegativity. Protection against rubella through natural infection appears inadequate to protect the population, increasing the risk of CRS.

  8. The human platelet alloantigen profile in blood donors from Amazonas, Brazil.

    Science.gov (United States)

    Portela, C N; Schriefer, A; Albuquerque, S R L; Perdomo, R T; Parente, A F A; Weber, S S

    2016-12-01

    Human platelet antigens (HPAs) are alloantigens derived from polymorphisms in platelet-surface glycoproteins. The occurrence of alloantibodies against HPAs can lead to platelet destruction and subsequent thrombocytopenia. Brazilians have a high rate of racial admixture, and the knowledge of HPA polymorphisms in particular donors from north Brazil, who have a large Amerindian influence, is a relevant strategy to prevent alloimmunisation. Our aim was investigate the HPA allele's frequencies in the Amazonas blood donors. We performed HPA genotyping among 200 Amazonas blood donors by microarray for 11 HPA biallelic systems, including six of the most clinically significant systems (HPA-1 to -5 and -15) and five others (HPA-6 to -9 and -11) that have been also associated with alloimmunisation, amounting to 22 HPA alleles. The obtained allele frequencies were compared with data of 38 populations worldwide to determine the hierarchical relationship and estimated the probability of mismatch platelets. The allele frequencies were 0·862 for HPA-1a, 0·137 for HPA-1b, 0·852 for HPA-2a, 0·147 for HPA-2b, 0·665 for HPA-3a, 0·335 for HPA-3b, 0·995 for HPA-4a, 0·005 for HPA-4b, 0·892 for HPA-5a, 0·107 for HPA-5b, 0·997 for HPA-9a, 0·005 for HPA-9b, 0·502 for HPA-15a and 0·497 for HPA-15b. The incompatibility risks are higher for HPA-15 and HPA-3, followed by HPA-1, -2 and -5. We found differences among populations worldwide, and it is interesting to note the indigenous and European influences in this region, reinforcing the heterogeneity in the ancestry of Brazilians. The results will be helpful in providing information for platelet transfusion to avoid alloimmunisation. © 2016 British Blood Transfusion Society.

  9. Non-detection of human herpesvirus 8 (HHV-8) DNA in HHV-8-seropositive blood donors from three Brazilian regions.

    Science.gov (United States)

    Levi, José Eduardo; Nascimento, Maria Claudia; Sumita, Laura Masami; de Souza, Vanda Akico Ueda Fick; Freire, Wilton S; Mayaud, Philippe; Pannuti, Claudio S

    2011-01-01

    Human herpesvirus 8 (HHV-8), also known as Kaposi's sarcoma-associated herpesvirus (KSHV), is the etiologic agent of all forms of Kaposi's sarcoma, primary effusion lymphoma and the plasmablastic cell variant of multicentric Castleman disease. In endemic areas of sub-Saharan Africa, blood transfusions have been associated with a substantial risk of HHV-8 transmission. By contrast, several studies among healthy blood donors from North America have failed to detect HHV-8 DNA in samples of seropositive individuals. In this study, using a real-time PCR assay, we investigated the presence of HHV-8 DNA in whole-blood samples of 803 HHV-8 blood donors from three Brazilian states (São Paulo, Amazon, Bahia) who tested positive for HHV-8 antibodies, in a previous multicenter study. HHV-8 DNA was not detected in any sample. Our findings do not support the introduction of routine HHV-8 screening among healthy blood donors in Brazil. (WC = 140).

  10. Sumatriptan increases the proliferation of peripheral blood mononuclear cells from HIV-infected individuals and healthy blood donors in vitro

    DEFF Research Database (Denmark)

    Afzelius, P; Nielsen, Jens Ole

    2000-01-01

    HIV infection is characterized by the loss of CD4+ T cells as well as the loss of T-cell function, leading to severe immunodeficiency. The proliferative capacity of T cells measured in vitro as responses to antigens and mitogens is severely reduced during HIV infection. An increased level...... responsible for regulation of the intracellular levels of cAMP. In a preliminary study sumatriptan increased the proliferative responses of PBMC to a polyclonal activator in vitro in 9 of 10 HIV-seropositive individuals (p=0.007), and in 7 of 9 healthy blood donors (p=0.05). This was probably due...... of the intracellular second messenger adenosine 3',5'-cyclic monophosphate (cAMP) has been shown to cause impaired proliferative capacity of peripheral blood mononuclear cells (PBMC) from HIV-infected individuals in vitro. Sumatriptan, a 5HT1d receptor agonist, inhibits the activity of adenylyl cyclases, the enzymes...

  11. ABO/Rh Blood Groups and Risk of HIV Infection and Hepatitis B Among Blood Donors of Abidjan, Côte D’ivoire

    Science.gov (United States)

    Siransy, Liliane Kouabla; Nanga, Zizendorf Yves; Zaba, Flore Sandrine; Tufa, Nyasenu Yawo; Dasse, Sery Romuald

    2015-01-01

    Hepatitis B and HIV infection are two viral infections that represent real global public health problems. In order to improve their management, some hypotheses suggest that genetic predispositions like ABO and Rh blood groups would influence the occurrence of these diseases. The aim of the present study was to examine the association between ABO and Rhesus blood groups and the susceptibility to HIV infection and hepatitis B. We conducted a cross-sectional and analytical study in a population of voluntary blood donors in the Blood Transfusion Center of Abidjan. All blood donors who donated blood between January and June 2014 were tested for HBs antigen and anti-HIV antibodies (ELISA tests) and were ABO typed. The total number of examined blood donors during this period was 45,538, of which 0.32% and 8.07% were respectively infected with HIV and hepatitis B virus. O-group donors were more infected than non-O donors. Our study is an outline concerning the search for a link between ABO and Rh blood groups and hepatitis B and HIV infection. Further studies should be conducted to confirm the interaction between these two infections and contribute to the search for new therapeutic approaches. PMID:26495131

  12. ABO/Rh Blood Groups and Risk of HIV Infection and Hepatitis B Among Blood Donors of Abidjan, Côte D'ivoire.

    Science.gov (United States)

    Siransy, Liliane Kouabla; Nanga, Zizendorf Yves; Zaba, Flore Sandrine; Tufa, Nyasenu Yawo; Dasse, Sery Romuald

    2015-09-01

    Hepatitis B and HIV infection are two viral infections that represent real global public health problems. In order to improve their management, some hypotheses suggest that genetic predispositions like ABO and Rh blood groups would influence the occurrence of these diseases. The aim of the present study was to examine the association between ABO and Rhesus blood groups and the susceptibility to HIV infection and hepatitis B. We conducted a cross-sectional and analytical study in a population of voluntary blood donors in the Blood Transfusion Center of Abidjan. All blood donors who donated blood between January and June 2014 were tested for HBs antigen and anti-HIV antibodies (ELISA tests) and were ABO typed. The total number of examined blood donors during this period was 45,538, of which 0.32% and 8.07% were respectively infected with HIV and hepatitis B virus. O-group donors were more infected than non-O donors. Our study is an outline concerning the search for a link between ABO and Rh blood groups and hepatitis B and HIV infection. Further studies should be conducted to confirm the interaction between these two infections and contribute to the search for new therapeutic approaches.

  13. The effect of clonidine infusion on distribution of regional cerebral blood flow in volunteers.

    Science.gov (United States)

    Bonhomme, Vincent; Maquet, Pierre; Phillips, Christophe; Plenevaux, Alain; Hans, Pol; Luxen, Andre; Lamy, Maurice; Laureys, Steven

    2008-03-01

    Through their action on the locus coeruleus, alpha2-adrenoceptor agonists induce rapidly reversible sedation while partially preserving cognitive brain functions. Our goal in this observational study was to map brain regions whose activity is modified by clonidine infusion so as to better understand its loci of action, especially in relation to sedation. Six ASA I-II right-handed volunteers were recruited. Electroencephalogram (EEG) was monitored continuously. After a baseline H2(15)O activation scan, clonidine infusion was started at a rate ranging from 6 to 10 microg x kg(-1) x h(-1). A sequence of 11 similar scans was then performed at 8 min intervals. Plasma clonidine concentration was measured. Using statistical parametric mapping, we sought linear correlations between normalized regional cerebral blood flow (rCBF), an indicator of regional brain activity, and plasma clonidine concentration or spindle EEG activity. Clonidine induced clinical sedation and EEG patterns (spindles) comparable to early stage nonrapid eye movement sleep. A significant negative linear correlation between clonidine concentration and rCBF or spindle activity was observed in the thalamus, prefrontal, orbital and parietal association cortex, posterior cingulate cortex, and precuneus. The EEG patterns and decreases in rCBF of specific brain regions observed during clonidine-induced sedation are similar to those of early stage nonrapid eye movement sleep. Patterns of deactivated brain regions are also comparable to those observed during general anesthesia or vegetative state, reinforcing the hypothesis that alterations in the activity of a common network occur during these modified conscious states.

  14. A spatial regression analysis of German community characteristics associated with voluntary non-remunerated blood donor rates.

    Science.gov (United States)

    Weidmann, C; Schneider, S; Litaker, D; Weck, E; Klüter, H

    2012-01-01

    Previous studies have shown substantial geographical variation in blood donation within developed countries. To understand this issue better, we identified community characteristics associated with blood donor rates in German municipalities in an ecological analysis. We calculated an aggregated rate of voluntary blood donors from each of 1533 municipalities in south-west Germany in 2007 from a database of the German Red Cross Blood Service. A multiple linear regression model estimated the association between the municipality-specific donor rate and several community characteristics. Finally, a spatial lag regression model was used to control for spatial autocorrelation that occurs when neighbouring units are related to each other. The spatial lag regression model showed that a relatively larger population, a higher percentage of inhabitants older than 30 years, a higher percentage of non-German citizens and a higher percentage of unemployed persons were associated with lower municipality-specific donor rates. Conversely, a higher donor rate was correlated with higher voter turnout, a higher percentage of inhabitants between 18 and 24 years and more frequent mobile donation sites. Blood donation appears to be a highly clustered regional phenomenon, suggesting the need for regionally targeted recruiting efforts and careful consideration of the value of mobile donation sites. Our model further suggests that municipalities with a decreasing percentage of 18- to 24-year-olds and an increasing percentage of older inhabitants may experience substantial declines in future blood donations. © 2011 The Author(s). Vox Sanguinis © 2011 International Society of Blood Transfusion.

  15. West Nile Virus (WNV seroprevalence in a blood donors group of Milan

    Directory of Open Access Journals (Sweden)

    Giovanna Lunghi

    2012-09-01

    Full Text Available A seroprevalence study for anti West Nile virus was carried out among 864 healthy blood donors living in the metropolitan area of Milan by using a commercially available ELISA method. In addition, the performance of a novel ELISA assay for WNV antibodies was assessed. The sero-prevalence rate of WNV antibodies was 0.57% thus showing that WNV is likely circulating also in this up to now unknown area. The overall sensitivity and specificity of the novel ELISA were 99.9% and 45.4%, respectively, well comparable with that of the chosen reference immunoenzimatic method.

  16. Seroepidemiology of human T-cell lymphotropic virus type-I in blood donors of Northeastern Iran, Sabzevar

    Directory of Open Access Journals (Sweden)

    Mahtab Maghsudlu

    2015-01-01

    Full Text Available Background and Objectives: Human T-cell lymphotropic virus type-I (HTLV-I infection is considered as a public health challenge in endemic areas. The virus is associated with severe diseases, such as adult T-cell leukemia/lymphoma, and HTLV-I-associated myelopathy/tropical spastic paraparesis. One of the major routes of the HTLV-I transmission includes blood transfusion. Sabzevar is located in the endemic region of HTLV-I infection. The aim of the present study was to determine the seroprevalence of HTLV-I infection in the blood donors in Sabzevar. Materials and Methods: A total of 35,067 blood donors in Sabzevar from March 2009 to April 2012 who were screened with HTLV-I on the enzyme-linked immunosorbent assay screening test were included in this survey. Reactive samples that confirmed by western blot were considered to be seropositive cases. The required data were obtained from blood donors′ database of blood transfusion service. Results: The overall prevalence of HTLV-1 based on the positive result of western blot test was 0.14%. The seropositive donors aged 17-59 years with a mean age of 38.10 ± 11.82. The prevalence rates of HTLV-I infection in 3 years of study were 0.19%, 0.14%, and 0.09%, respectively. A significant relation between age, sex, educational level, and history of blood donation was observed with seropositivity of HTLV-I. Conclusion: The improvement of donor selection and laboratory screening caused a decline in the prevalence of infection in blood donors. Given the lower prevalence of infection in regular donors with lower age and higher educational level, more efforts should be done to attract blood donors from these populations.

  17. Sibling cord blood donor program for hematopoietic cell transplantation: the 20-year experience in the Rome Cord Blood Bank.

    Science.gov (United States)

    Screnci, Maria; Murgi, Emilia; Valle, Veronica; Tamburini, Anna; Pellegrini, Maria Grazia; Strano, Sabrina; Corona, Francesca; Ambrogi, Eleonora Barbacci; Girelli, Gabriella

    2016-03-01

    Umbilical cord blood (UCB) represents a source of hematopoietic stem cells for patients lacking a suitably matched and readily available related or unrelated stem cell donor. As UCB transplantation from compatible sibling provides good results in children therefore directed sibling UCB collection and banking is indicated in family who already have a child with a disease potentially treatable with an allogeneic hematopoietic stem cell transplantation. Particularly, related UCB collection is recommended when the patients urgently need a transplantation. To provide access to all patients in need, we developed a "Sibling cord blood donor program for hematopoietic cell transplantation". Here we report results of this project started 20years ago. To date, in this study a total of 194 families were enrolled, a total of 204 UCB samples were successfully collected and 15 pediatric patients have been transplanted. Recently, some authors have suggested novel role for UCB other than in the transplantation setting. Therefore, future studies in the immunotherapy and regenerative medicine areas could expand indication for sibling directed UCB collection.

  18. [Giving blood: an individual right or the expression of a social responsibility? The donors' position in the debates on blood transfusion].

    Science.gov (United States)

    Bastard, B

    2006-09-01

    This debate questions the donor's position within the transfusional framework. How are blood donation regulations initiated and implemented? Experts called upon this question insist on the necessity to better inform about such regulations so that they can be rapidly approved and in order to keep donors from adverse reactions linked to blood donations. How to make donors' rejections more tolerable? Contra-indications are a source of anxiety for the person whose blood sample is not accepted. While blood transfusion has never been safer in the past than it is today in wealthy countries, one should ask the question whether the donor is or not the victim of doubts still weighing down on this activity. Implementing the precautionary principle may indeed lead transfusion supervisors to take brutal decisions detrimental to the donor, seeming rather inspired by political or media pressures than calling on scientific or economic rigorous principles. What does the donor selection aim at in terms of prevention and public health? What about the renewal of donor associations' directors? Where are we at with the homosexual rejection trends? Such are the issues as those induced by the current debate.

  19. Ancestry informative markers and complete blood count parameters in Brazilian blood donors

    Directory of Open Access Journals (Sweden)

    Gabriela E. S. Felix

    Full Text Available A complete blood count is very useful in clinical diagnoses when reference ranges are well established for the population. Complete blood counts and allele frequencies of Ancestry Informative Markers (AIMs were analyzed in Brazilians with the aim of characterizing the hematological values of an admixed population. Positive associations were observed between gender and neutrophils, monocytes, eosinophils, erythrocytes, hemoglobin, hematocrit, MCV, MCHC and platelet counts. No significant differences were found for age, alcohol consumption, educational status, ethnicity, smoking in respect to the complete blood count values. In general, men had higher red blood cell values, while women had higher values for white blood cells and platelets. The study of the population was highly heterogeneous with mean proportions (± SE of African, European and Amerindian ancestry being 49.0 ± 3.0%, 44.0 ± 9.0% and 7.0 ± 9.0%, respectively. Amerindian ancestry showed limited contribution to the makeup of the population, but estimated ancestral proportions were statistically significant (r = 0.9838; P<0.001. These hematologic values are similar to Afro-Americans, another admixed population.

  20. Seroprevalencia de toxocarosis en donantes de sangre Toxocarosis seroprevalence in blood donors

    Directory of Open Access Journals (Sweden)

    N. Navarrete

    1998-01-01

    Full Text Available Se determinó, mediante la técnica de ELISA, la seroprevalencia de toxocarosis en 188 donantes de sangre del Hospital Base de Valdivia. El 74.5% de los donantes fueron del sexo masculino y el 25.5% del sexo femenino, con edad promedio de 29.9 ± 8.5 años. Se demostró la presencia de anticuerpos anti-Toxocara en el 5.3% de los donantes. El análisis estadístico no mostró diferencias significativas para la prevalencia de infección en relación a las variables de sexo, edad y procedencia del huésped.Toxocarosis seroprevalence in 188 blood donors of the County Hospital of Valdivia was determined using the ELISA test. 74.5% of the donors were male and 25.5% female, with an age average of 29.9 ± 8.5. Anti Toxocara antibodies were detected in 5.3% of the donors, and no significant differences were observed in relation to sex, age or origin

  1. Dengue antibodies in blood donors Pesquisa de anticorpos antidengue em doadores de sangue

    Directory of Open Access Journals (Sweden)

    Rejane Cristina Ribas-Silva

    2012-01-01

    Full Text Available BACKGROUND: Dengue is an urban arbovirus whose etiologic agent is a virus of the genus Flavorius with four distinct antigen serotypes (DENV-1, DENV-2, DENV-3 and DENV-4 that is transmitted to humans through the bite of the mosquito Aedes aegypti. The Campo Mourão region in Brazil is endemic for dengue fever. OBTECTIVE: The aim of this study was to evaluate the presence of IgG and IgM antibodies specific to the four serotypes of dengue in donors of the blood donor service in the city of Campo Mourão. METHODS: Epidemiological records were evaluated and 4 mL of peripheral blood from 213 blood donors were collected in tubes without anticoagulant. Serum was then obtained and immunochromatographic tests were undertaken (Imuno-Rápido Dengue IgM/IgG TM. Individuals involved in the study answered a social and epidemiological questionnaire on data which included age, gender and diagnosis of dengue. RESULTS: Only three (1.4% of the 213 blood tests were positive for IgG anti-dengue antibodies. No donors with IgM antibody, which identifies acute infection, were identified. CONCLUSIONS: The results of the current analysis show that the introduction of quantitative or molecular serological methods to determine the presence of anti-dengue antibodies or the detection of the dengue virus in blood donors in endemic regions should be established so that the quality of blood transfusions is guaranteed.OBJETIVO: O objetivo desta pesquisa foi realizar uma triagem sorológica para detectar a presença de anticorpos IgG e IgM específicos para os quatro sorotipos da dengue em doadores de sangue atendidos pelo Hemonúcleo de Campo Mourão-PR. MÉTODOS: foram aplicadas fichas epidemiológicas e coletados 4 mL de sangue periférico em tubos sem anticoagulante de 213 doadores de sangue. Posteriormente, foi obtido o soro e realizado o teste imunocromatrográfico (Imuno - Rápido Dengue IgM/IgG. Os indivíduos envolvidos neste estudo responderam um questionário s

  2. Temperature-dependent haemolytic propensity of CPDA-1 stored red blood cells vs whole blood - Red cell fragility as donor signature on blood units.

    Science.gov (United States)

    Tzounakas, Vassilis L; Anastasiadi, Alkmini T; Karadimas, Dimitrios G; Zeqo, Redisa A; Georgatzakou, Hara T; Pappa, Olga D; Papatzitze, Olga A; Stamoulis, Konstantinos E; Papassideri, Issidora S; Antonelou, Marianna H; Kriebardis, Anastasios G

    2017-09-01

    To preserve cellular integrity and avoid bacterial growth, storage and transfer of blood and blood products follow strict guidelines in terms of temperature control. We evaluated the impact of ineligible warming of whole blood donations on the quality of blood components. One-hundred and twenty units of whole blood (WB) from eligible blood donors were collected in CPDA-1 and stored at 4±2 °C. During shipment to the blood processing centre, a gradual warming up to 17 °C was recorded within a period of less than eight hours. The warmed units were processed to packed red blood cells (PRBCs) or stored as WB units at 4±2 °C. In-bag haemolysis, osmotic fragility (mean corpuscular fragility, MCF) and bacterial growth were assessed in blood and blood components throughout the storage period. Normal basal and early storage levels of haemolysis were recorded in both PRBC and WB units. Thereafter, PRBCs exhibited higher average in-bag haemolysis and MCF index compared to the WB units throughout the storage. Moreover, 14.3 and 52.4% of the PRBC units exceeded the upper permissible limit of 0.8% haemolysis at the middle (1.220±0.269%) or late (1.754±0.866%) storage period, respectively. MCF index was similar in all PRBCs at the middle of storage but significantly lower in the non-haemolysed compared to the haemolysed units of PRBCs on the last days. The fragility of stored RBCs was proportional to the donor-related values of day 2 samples (r=0.861, punits of PRBCs. Transient, gradient warming of whole blood from 4 to 17 °C led to increased incidence of in-bag haemolysis in PRBC but not in WB units. Haemolysis is a multi-parametric phenotype of stored blood, and MCF is a donor-related and highly dynamic measure that can, in part, predict the storage lesion.

  3. Preoperative predictors of blood component transfusion in living donor liver transplantation

    Directory of Open Access Journals (Sweden)

    R N Makroo

    2013-01-01

    Full Text Available Context: Extensive bleeding associated with liver transplantation is a major challenge faced by transplant surgeons, worldwide. Aims: To evaluate the blood component consumption and determine preoperative factors that predict the same in living donor liver transplantation (LDLT. Settings and Design: This prospective study was performed for a 1 year period, from March 2010 to February 2011. Materials and Methods: Intra- and postoperative utilization of blood components in 152 patients undergoing LDLT was evaluated and preoperative patient parameters like age, gender, height, weight, disease etiology, hemoglobin (Hb, hematocrit (Hct, platelet count (Plt, total leukocyte count (TLC, activated partial thromboplastin time (aPTT, international normalized ratio (INR, serum bilirubin (T. bilirubin, total proteins (T. proteins, albumin to globulin ratio (A/G ratio, serum creatinine (S. creatinine, blood urea (B. urea, and serum electrolytes were assessed to determine their predictive values. Univariate and stepwise discriminant analysis identified those factors, which could predict the consumption of each blood component. Results: The average utilization of packed red cells (PRCs, cryoprecipitates (cryo, apheresis platelets, and fresh frozen plasma was 8.48 units, 2.19 units, 0.93 units, and 2,025 ml, respectively. Disease etiology and blood component consumption were significantly correlated. Separate prediction models which could predict consumption of each blood component in intra and postoperative phase of LDLT were derived from among the preoperative Hb, Hct, model for end-stage liver disease (MELD score, body surface area (BSA, Plt, T. proteins, S. creatinine, B. urea, INR, and serum sodium and chloride. Conclusions: Preoperative variables can effectively predict the blood component requirements during liver transplantation, thereby allowing blood transfusion services in being better prepared for surgical procedure.

  4. Hepatitis B surface antigen variants in voluntary blood donors in Nanjing, China

    Science.gov (United States)

    2012-01-01

    Background Hepatitis B virus (HBV) is still one of the serious infectious risks for the blood transfusion safety in China. One plausible reason is the emergence of the variants in the major antigenic alpha determinant within the major hydrophilic region (MHR) of hepatitis B surface antigen (HBsAg), which have been assumed to evade the immune surveillance and pose a challenge to the disease diagnosis. It is well documented that some commercial ELISA kits could detect the wild-type but not the mutant viruses. The high prevalence of HBV in China also impaired the application of nucleic acid testing (NAT) in the improvement of blood security. Molecular epidemiological study of HBsAg variations in China is still limited. This study was designed to identify the prevalence of mutations in the HBsAg in voluntary blood donors in Nanjing, China. Methods A total of 20,326 blood units were enrolled in this study, 39 donors were positive for HBV S gene in the nested-PCR. Mutations in the major hydrophilic region (MHR; aa 99-169) were identified by direct sequencing of S region. Results Among of 20,326 blood units in the Red Cross Transfusion Center of Nanjing from October 2008 to April 2009, 296 samples (1.46%, 296/20,326) were HBsAg positive in the 2 successive rounds of the ELISA test. In these HBsAg positive units, HBV S gene could be successfully amplified from 39 donors (13.18%, 39/296) in the nested-PCR. Sequence analysis revealed that 32 strains (82.1%, 32/39) belong to genotype B, 7 strains (17.9%, 7/39) to genotype C. Besides well known G145R, widely dispersed variations in the MHR of S region, were observed in 20 samples of all the strains sequenced. Conclusions HBV/B and HBV/C are dominant in Nanjing, China. The mutations in the MHR of HBsAg associated with disease diagnosis are common. PMID:22500577

  5. Hepatitis B surface antigen variants in voluntary blood donors in Nanjing, China

    Directory of Open Access Journals (Sweden)

    Yong-lin Yang

    2012-04-01

    Full Text Available Abstract Background Hepatitis B virus (HBV is still one of the serious infectious risks for the blood transfusion safety in China. One plausible reason is the emergence of the variants in the major antigenic alpha determinant within the major hydrophilic region (MHR of hepatitis B surface antigen (HBsAg, which have been assumed to evade the immune surveillance and pose a challenge to the disease diagnosis. It is well documented that some commercial ELISA kits could detect the wild-type but not the mutant viruses. The high prevalence of HBV in China also impaired the application of nucleic acid testing (NAT in the improvement of blood security. Molecular epidemiological study of HBsAg variations in China is still limited. This study was designed to identify the prevalence of mutations in the HBsAg in voluntary blood donors in Nanjing, China. Methods A total of 20,326 blood units were enrolled in this study, 39 donors were positive for HBV S gene in the nested-PCR. Mutations in the major hydrophilic region (MHR; aa 99-169 were identified by direct sequencing of S region. Results Among of 20,326 blood units in the Red Cross Transfusion Center of Nanjing from October 2008 to April 2009, 296 samples (1.46%, 296/20,326 were HBsAg positive in the 2 successive rounds of the ELISA test. In these HBsAg positive units, HBV S gene could be successfully amplified from 39 donors (13.18%, 39/296 in the nested-PCR. Sequence analysis revealed that 32 strains (82.1%, 32/39 belong to genotype B, 7 strains (17.9%, 7/39 to genotype C. Besides well known G145R, widely dispersed variations in the MHR of S region, were observed in 20 samples of all the strains sequenced. Conclusions HBV/B and HBV/C are dominant in Nanjing, China. The mutations in the MHR of HBsAg associated with disease diagnosis are common.

  6. Human-induced pluripotent stem cells from blood cells of healthy donors and patients with acquired blood disorders.

    Science.gov (United States)

    Ye, Zhaohui; Zhan, Huichun; Mali, Prashant; Dowey, Sarah; Williams, Donna M; Jang, Yoon-Young; Dang, Chi V; Spivak, Jerry L; Moliterno, Alison R; Cheng, Linzhao

    2009-12-24

    Human induced pluripotent stem (iPS) cells derived from somatic cells hold promise to develop novel patient-specific cell therapies and research models for inherited and acquired diseases. We and others previously reprogrammed human adherent cells, such as postnatal fibroblasts to iPS cells, which resemble adherent embryonic stem cells. Here we report derivation of iPS cells from postnatal human blood cells and the potential of these pluripotent cells for disease modeling. Multiple human iPS cell lines were generated from previously frozen cord blood or adult CD34(+) cells of healthy donors, and could be redirected to hematopoietic differentiation. Multiple iPS cell lines were also generated from peripheral blood CD34(+) cells of 2 patients with myeloproliferative disorders (MPDs) who acquired the JAK2-V617F somatic mutation in their blood cells. The MPD-derived iPS cells containing the mutation appeared normal in phenotypes, karyotype, and pluripotency. After directed hematopoietic differentiation, the MPD-iPS cell-derived hematopoietic progenitor (CD34(+)CD45(+)) cells showed the increased erythropoiesis and gene expression of specific genes, recapitulating features of the primary CD34(+) cells of the corresponding patient from whom the iPS cells were derived. These iPS cells provide a renewable cell source and a prospective hematopoiesis model for investigating MPD pathogenesis.

  7. [A large-scale survey for rare blood group screening among blood donors in Chinese over Nanjing area].

    Science.gov (United States)

    Ma, Ling; Liu, Yan-Chun; Xue, Min; Wei, Peng; Tang, Rong-Cai

    2011-02-01

    The purpose of this study was to investigate the distribution of 10 rare red blood groups in Chinese Nanjing population, so as to provide compatible rare blood to patients and to create a donor data bank. Jk (a-b-) (Kidd) phenotypes were detected by urea, while H-(H), GPA-(MNS), GPC-(Gerbich), i+ (Ii) and Lub-(Lutheran) phenotypes were detected by monoclonal, polyclonal antibodies with U type 96 well microplate technology. The screening of Jsb- and k-(Kell), Fya-(Duffy), Ok-(Ok), s-(MNS) and Dib-(Digeo) phenotypes were performed by polymerase chain reaction. The results showed that 2 Jk (a-b-) out of 40337 donation samples and 3 Fy (a-b+) out of 1782 donation samples were found, while no other rare blood phenotypes (H-, GPA-, GPC-, Lub-, Ok-, s-, Jsb-, k-, Dib- and i+) were detected. It is concluded that the frequencies of Jk (a-b-) and Fya(a-b+) are 0.0049% and 0.168% respectively. No more rare blood phenotype was found in this screening.

  8. Detection of hepatitis C virus (HCV) core-specific antibody suggests occult HCV infection among blood donors.

    Science.gov (United States)

    Quiroga, Juan A; Avellón, Ana; Bartolomé, Javier; Andréu, María; Flores, Elena; González, María I; González, Rocío; Pérez, Sonia; Richart, Luis A; Castillo, Inmaculada; Alcover, Javier; Palacios, Ricardo; Carreño, Vicente; Echevarría, José M

    2016-07-01

    Blood transfusion safety is based on reliable donor screening for transmissible infections such as the hepatitis C virus (HCV) infection. A novel HCV core-specific antibody was assayed on random single donations from 2007 first-time blood donors who tested negative for anti-HCV and HCV RNA on routine screening. Sample collection broke the code between donations and donors for ethical reasons. Forty-two donations (2.1%) displayed reactivity in the novel test. The specificity of the reactivity was evaluated by a peptide inhibition assay, and testing against additional nonoverlapping HCV core peptide epitopes and other HCV antigens was performed on these samples. Six donations (14.3%; 0.30% from the total) were considered to contain anti-HCV after such supplemental testing. HCV RNA detection was also performed in peripheral blood mononuclear cells (PBMNCs) and serum or plasma samples from reactive donors after virus concentration by ultracentrifugation. HCV RNA tested negative in all PBMNCs samples, and a very low amount of viral genome was detected in serum or plasma concentrates from three anti-HCV core-reactive donors (7.1%) but not among concentrates from 100 randomly selected nonreactive donors. Sequencing of these polymerase chain reaction products revealed differences between the isolates that excluded partially sample contamination from a common source. These findings argue in favor of an ongoing occult HCV infection among these blood donors and account for some rather low, but perhaps not negligible, infection risk for such donations. Future studies involving larger samples of donations from traceable donors would enlighten the significance of these findings for the viral safety of the blood supply. © 2016 AABB.

  9. Distribution of Hepatitis B virus genotypes among healthy blood donors in eastern part of North India

    Directory of Open Access Journals (Sweden)

    Kumar Kailash

    2011-01-01

    Full Text Available Aim: We evaluated the distribution HBV genotypes among non-remunerated healthy blood donors in eastern North India. Materials and Methods: During screening of donated blood, 176 consecutive HBsAg positive, samples comprised the study. HBV-DNA was quantitative detected in 150 samples by PCR. HBV genotype was determined by identifying genotype-specific DNA band using nested PCR. Results: Majorities were of age group 31-40 yrs (65.3%. Males (92.7% outnumbered females (7.3% and were HbeAg-negative HBsAg carriers. Over all, genotype-A was the most prevalent (54% followed by D (21.3%. We did not find genotype-G and H. Districts under study, divided into four zones: Zone-I genotype-A was most common (62.3% followed by D (18.8%; Zone-II genotype-C (41.2% was more frequent followed by D (20.6% and A (17.7%. Zone-III in adjoining Bihar state close to Zone-I, A was more prevalent (81.8% followed by B and C (9.1%. In Zone-IV adjoining Zone- II had genotype-A (100% only. Genotype-D had more sporadic distribution. Genotype-E and F were prevalent in Zone I and II (3/150, 2%. Conclusions: Among blood donors HBV genotype-A followed by D was the most prevalent in eastern North India. Genotype-A had pattern of distribution signifying common focus, while D was more sporadic and C had single large pocket (Zone-II probably common focus but restricting to particular area. Evidences are suggestive of association of HBV genotype in liver dysfunction. An effective treatment and preventive strategies based of genotypes will reduce the disease burden and increase the blood safety.

  10. Prevalence and factors associated with hepatitis C virus infection among Myanmar blood donors.

    Science.gov (United States)

    Myo-Khin; San-San-Oo; Oo, Khin May; Shimono, Kunio; Koide, Norio; Okada, Shigeru

    2010-10-01

    We studied the prevalence of hepatitis C virus infection among blood donors from 3 hospitals of Central Myanmar and 7 hospitals of Lower Myanmar in the Yangon area, and analyzed the factors associated with the infection. The study period was from November, 2005 to June, 2007. A pre-tested questionnaire was used to obtain information on age, ethnic group, marital status, tattooing, body piercing, history of receiving transfusions, and liver diseases in self and in sexual partners. Data on seropositivity to hepatitis C, hepatitis B and human immunodeficiency virus infections were recorded. A total of 65,240 blood donors participated in the study. Their ages ranged from 18 years to 60 years (mean±SD=29.5±9.3). The male-to-female ratio was 6:1. The prevalence of the antibody to hepatitis C was found to be 0.95% with varying rates (0.34 to 2.03) among hospitals. Females had a slightly higher rate (1.06%) than males (0.93%) (p=0.237). Multivariate analyses revealed the following factors to be related to HCV infection:HIV infection, odds ratio (OR)=3.0 (p=0.003); history of liver disease, OR=8.9 (p=0.001);and age 30 years and above, OR=2.6 (p=0.001). We discuss the varying prevalences of HCV around the world.

  11. Healthy ranges of serum alanine aminotransferase levels in Tranian blood donors

    Institute of Scientific and Technical Information of China (English)

    Mehdi Mohamadnejad; Akram Pourshams; Reza Malekzadeh; Ashraf Mohamadkhani; Afsaneh Rajabiani; Ali Ali Asgari; Seyed Meysam Alimohamadi; Hadi Razjooyan; Mamar-Abadi

    2003-01-01

    AIM:The healthy ranges for serum alanine aminotransferase (ALT) levels are less well studied. The aim of this study was to define the upper limit of normal (ULN) for serum ALT levels, and to assess factors associated with serum ALT activity in apparently healthy blood donors.METHODS: A total of 1 939 blood donors were included.ALT measurements were performed for all cases using the same laboratory method. Healthy ranges for ALT levels were computed from the population at the lowest risk for liver disease. Univariate and multivariate analyses were performed to evaluate associations between clinical factors and ALT levels.RESULTS: Serum ALT activity was independently associated with body mass index (BMI) and male gender, but not associated with age. Association of ALT with BMI was more prominent in males than in females. Upper limit of normal for non-overweight women (BMI of less than 25) was 34 U/L,and for non-overweight men was 40 U/L.CONCLUSION: Serum ALT is strongly associated with sex and BMI. The normal range of ALT should be defined for male and female separately.

  12. Challenges of using HIV as a primary risk indicator: Need for integrated blood donor risk management model

    NARCIS (Netherlands)

    Mapako, T.; Parirewa, J.J.; Emmanuel, J.C.; Mvere, D.A.; Massundah, E.; Mavunganidze, G.; Marowa, L.M.; Postma, M.J.; Van Hulst, M.

    2015-01-01

    Background: The use of risk modelling in blood safety is increasing getting momentum. NBSZ initiated blood donor risk profiling based on donation frequency (r-coding) since 1994 and in 2006 a generic risk classification model was developed (include age and donation venue) which was mainly based on H

  13. [Contributions of the Council of Europe's Blood Transfusion Steering Committee to the determination of rules for the selection of donors of blood and blood components and the study of sexual behaviors having an impact on blood safety].

    Science.gov (United States)

    Behr-Gross, M-E; Heiden, M; Norda, R

    2013-05-01

    In November 2009, the Council of Europe's Blood Transfusion Steering Committee created a group of experts to explore the problem of behaviors having an impact on the management of donors of blood and blood components and on blood transfusion safety in Europe. This ad hoc group sought a harmonised interpretation of temporary exclusion (or temporary deferral), as opposed to permanent exclusion (or permanent deferral), in the context of the selection of donors of blood and blood components. It was also given the mandate to assess, on the basis of available data, the possibility of differentiating "at risk" behaviours from behaviours "at high risk" of contamination by serious infectious diseases transmitted by blood, blood components or derived therapeutic products. The primary objective of this work was to ensure the safety of blood, blood components and derived therapeutic products for future recipients by promoting a risk analysis-based approach, given that some countries envisaged amending their provisions for donor selection. However, a risk analysis can only be performed on groups, not individuals, which may give the impression of a discriminatory approach, so it needed to be justified in the context of transfusion safety. A collaborative project, which included an investigation phase, led to the drafting of a technical memorandum that summarised the data collected in ten Council of Europe member states on the selection criteria for blood donors and the epidemiology of infectious diseases (with a focus on human immunodeficiency virus) in the general population and among blood donors. The technical memorandum was published in 2011 on the European Directorate for the Quality of Medicines and Healthcare website dedicated to this project. A draft resolution of the Committee of Ministers of the Council of Europe was then developed by the Council of Europe's Blood Transfusion Steering Committee. This text was circulated among member and observer states of the Council

  14. Quantification of print, radio and television exposure among previous blood donors in Kenya: an opportunity for encouraging repeat donation in a resource-limited setting?

    Science.gov (United States)

    Basavaraju, S V; Mwangi, J; Kellogg, T A; Odawo, L; Marum, L H

    2010-10-01

    Blood services in sub-Saharan Africa experience blood shortages and low retention of voluntary, non-remunerated donors. To boost collections by encouraging repeat donations, the Kenya National Blood Transfusion Service is exploring the likelihood of reaching previous donors through targeted print, radio and television advertising. We analysed data from a national AIDS Indicator Survey to determine whether previous donors have significant exposure to media. Respondents reporting history of blood donation had significantly higher exposure to print, radio and television media than those without history of blood donation. Targeted media campaigns encouraging repeat donation are likely to reach previous donors even in resource-limited settings.

  15. Infectivity of blood components from donors with occult hepatitis B infection - results from an Australian lookback programme.

    Science.gov (United States)

    Seed, C R; Maloney, R; Kiely, P; Bell, B; Keller, A J; Pink, J

    2015-02-01

    Previous studies have demonstrated that transfused blood components from donors with occult hepatitis B virus infection (OBI) are potentially infectious. This study reports the results of an Australian lookback programme for the period subsequent to the commencement of individual donation HBV NAT in July 2010 and estimates the HBV transmission rate for components from two categories of donors, confirmed OBI and HBV inconclusive (anti-HBc reactive with non-discriminated NAT result). Using the results of lookback investigations, we estimated HBV transmission rates by donor category and type of component transfused based on the prevalence of antibodies to HBV core antigen (anti-HBc) in recipients adjusted for the estimated prevalence in the general population. After subtracting the background anti-HBc rate, we derived an adjusted transmission rate (all components) with lower and upper bounds as follows: 0·85% (0·00-2·35%) for OBI donors, 2·83% (1·23-4·33%) for inconclusive donors and 1·81% (0·21-3·31%) for total (OBI and inconclusive) donors. The median adjusted transmission rate for total donors was higher (but not statistically) for plasma (3·01%) than RCCs (2·86%), but there was no evidence of transmission for cryoprecipitate or platelets (0% for both components). Our lookback study suggests a low (0·2-3·3%) but measurable rate of HBV transmission in Australia associated with donors with OBI and supports published evidence that at least some blood component types from OBI donors, including a proportion undetectable by ID-NAT can transmit HBV by transfusion. © 2014 International Society of Blood Transfusion.

  16. Umbilical cord blood units for public storage donors screening for markers of infectious agents

    Directory of Open Access Journals (Sweden)

    A. B. Smolyaninov

    2012-01-01

    Full Text Available The screening of 3515 cord blood samples which had entered bank for public use for presence of markers of infectious agents was carried out. It was established that majority of cord blood units contain markers of cytomegalovirus and Toxoplasma (81% that is not a contraindication for storage and subsequent use of these samples. In 4.6% of cases umbilical cord blood units were subjected to disposal because of identification of viral hepatitis B and C, as well as Tr. pallidum markers, moreover, the largest share of the discarded units contained antibodies to HbscorAg – 71,3%. Inclusion of analysis on the presence of Anti-HBcor in the required laboratory screening of mothers-donors CB was proposed in order to reduce the percentage of discarded umbilical cord blood units for the public inventory.

  17. The heritability of blood donation

    DEFF Research Database (Denmark)

    Pedersen, Ole Birger; Axel, Skytthe; Rostgaard, Klaus

    2015-01-01

    BACKGROUND: Voluntary blood donation is believed to be mostly motivated by altruism. Because studies have suggested that altruistic personality is determined by both genetic and environmental factors, we speculated that willingness to donate blood could also be governed by constitutional factors...... active Danish blood donors from 2002 to 2012, to establish blood donor status for Danish twins, who at age 17 years became eligible for donation in 2002 or later. Casewise concordance in monozygotic (MZ) and dizygotic (DZ) twins were presented and heritability was estimated in Mx by variance component...... to donate blood, respectively. CONCLUSION: Becoming a volunteer blood donor is determined by both genetic and environmental factors shared within families....

  18. Serum γ-glutamyltransferase, alanine aminotransferase, and aspartate aminotransferase activity in Iranian healthy blood donor men

    Institute of Scientific and Technical Information of China (English)

    Hossein Khedmat; Nasrin Zarei; Farahnaz Fallahian; Hassan Abolghasemi; Bashir Hajibeigi; Zohre Attarchi; Farshid Alaeddini; Mohammad Taghi Holisaz; Masoumeh Pourali; Shahin Sharifi

    2007-01-01

    AIM: To determine serum γ-glutamyltransferase (GGT), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) activity, and to assess their correlation with demographic and clinical findings in healthy blood donors.METHODS: This cross-sectional study was performed in 934 male blood donors, aged 18 to 68 years, who consecutively attended Tehran blood transfusion service in 2006. All participants were seronegative for HBV or HCV infections, non alcohol users, and all underwent a standard interview and anthropometric tests. Clinical and biochemical parameters including AST, ALT, and GGT activities were determined. Patients taking drugs known to cause hepatic fat deposition were excluded. For AST, ALT, and GGT variables, we used 33.33 and 66.66 percentiles, so that each of them was divided into three tertiles.RESULTS: Mean AST, ALT, and GGT activities were 25.26 ± 12.58 U/L (normal range 5-35 U/L), 33.13 ± 22.98 (normal range 5-35 U/L), and 25.11 ± 18.32 (normal range 6-37 U/L), respectively. By univariate analyses, there were significant associations between increasing AST, ALT, or GGT tertiles and age, body weight, body mass index, and waist and hip circumferences (P < 0.05). By multiple linear regression analyses, ALT was found to be positively correlated with dyslipidemia (B = 6.988, P = 0.038), whereas ALT and AST were negatively correlated with age. AST, ALT, and GGT levels had positive correlation with family history of liver disease (B = 15.763, P < 0.001), (B = 32.345, P < 0.001), (B =24.415, P < 0.001), respectively.CONCLUSION: Although we did not determine the cutoffs of the upper normal limits for AST, ALT, and GGT levels, we would suggest screening asymptomatic patients with dyslipidemia and also subjects with a family history of liver disease.

  19. Increased mortality associated with HTLV-II infection in blood donors: a prospective cohort study

    Directory of Open Access Journals (Sweden)

    Smith James W

    2004-03-01

    Full Text Available Abstract Background HTLV-I is associated with adult T-cell leukemia, and both HTLV-I and -II are associated with HTLV-associated myelopathy/tropical spastic paraparesis (HAM/TSP. Several published reports suggest that HTLV-I may lead to decreased survival, but HTLV-II has not previously been associated with mortality. Results We examined deaths among 138 HTLV-I, 358 HTLV-II, and 759 uninfected controls enrolled in a prospective cohort study of U.S. blood donors followed biannually since 1992. Proportional hazards models yielded hazard ratios (HRs for the association between mortality and HTLV infection, controlling for sex, race/ethnicity, age, income, educational level, blood center, smoking, injection drug use history, alcohol intake, hepatitis C status and autologous donation. After a median follow-up of 8.6 years, there were 45 confirmed subject deaths. HTLV-I infection did not convey a statistically significant excess risk of mortality (unadjusted HR 1.9, 95%CI 0.8–4.4; adjusted HR 1.9, 95%CI 0.8–4.6. HTLV-II was associated with death in both the unadjusted model (HR 2.8, 95%CI 1.5–5.5 and in the adjusted model (HR 2.3, 95%CI 1.1–4.9. No single cause of death appeared responsible for the HTLV-II effect. Conclusions After adjusting for known and potential confounders, HTLV-II infection is associated with increased mortality among healthy blood donors. If replicated in other cohorts, this finding has implications for both HTLV pathogenesis and counseling of infected persons.

  20. Successful unintentional ABO-incompatible renal transplantation: Blood group A1B donor into an A2B recipient.

    Science.gov (United States)

    Fadeyi, Emmanuel A; Stratta, Robert J; Farney, Alan C; Pomper, Gregory J

    2014-05-01

    To report a successful unintentional transplantation of a deceased donor kidney from an "incompatible" A1B donor into a recipient who was blood group A2B with unsuspected preformed anti-A1 antibodies. The donor and recipient were both typed for ABO antigens. The recipient was tested for ABO and non-ABO antibodies. The recipient was typed for HLA class I and class II antigens, including HLA antibody screen. The T-and B-flow cytometry crossmatch test was performed using standard protocol. The donor-recipient pair was a complete six-antigen human leukocyte antigen mismatch, but final T- and B-flow cytometry cross-match tests were compatible. The recipient was a 65-year-old woman with a medical history of end-stage renal disease secondary to diabetic nephropathy who underwent kidney transplantation from a 46-year-old brain-dead standard criteria donor. The recipient's RBCs were negative with A1 lectin, and the recipient was thus typed as an A2 subgroup. Anti-A1 could be demonstrated in the recipient's plasma. The donor's RBCs were positive with A1 lectin, thereby conferring an A1 blood type. It is safe to transplant across the A1/A2 blood group barrier provided that the preformed antibodies are not reactive at 37°C and with anti-human globulin.

  1. Blood donor screening for West Nile virus (WNV) revealed acute Usutu virus (USUV) infection, Germany, September 2016

    Science.gov (United States)

    Cadar, Daniel; Maier, Philipp; Müller, Susanne; Kress, Julia; Chudy, Michael; Bialonski, Alexandra; Schlaphof, Alexander; Jansen, Stephanie; Jöst, Hanna; Tannich, Egbert; Runkel, Stefan; Hitzler, Walter E; Hutschenreuter, Gabriele; Wessiepe, Martina; Schmidt-Chanasit, Jonas

    2017-01-01

    Between 1 June and 31 December 2016, 13,023 blood donations from the University Hospital Aachen in Germany were routinely screened for West Nile virus (WNV) RNA using the cobas TaqScreen WNV Test. On 28 September 2016, one blood donor was tested positive. Subsequent analysis revealed an acute Usutu virus (USUV) infection. During the ongoing USUV epizootics in Germany, blood transfusion services, public health authorities and clinicians should be aware of increased human USUV infections. PMID:28422005

  2. Evaluation of positive and false-positive results in syphilis screening of blood donors in Rio de Janeiro, Brazil.

    Science.gov (United States)

    Sandes, V S; Silva, S G C; Motta, I J F; Velarde, L G C; de Castilho, S R

    2017-06-01

    We propose to analyse the positive and false-positive results of treponemal and nontreponemal tests in blood donors from Brazil and to evaluate possible factors associated with the results of treponemal tests. Treponemal tests have been used widely for syphilis screening in blood banks. The introduction of these tests in donor screening has caused an impact and a loss of donors who need to be assessed. This was a retrospective cross-sectional study of syphilis screening and confirmatory test results of blood donors that were obtained before and after adopting a chemiluminescent immunoassay (CLIA). A comparative analysis was performed using a second sample drawn from positive donors. The possible factors associated with CLIA-positive or CLIA-false-positive results were investigated in a subgroup. Statistical tests were used to compare the proportions and adjusted estimates of association. The reactivity rate increased from 1·01% (N = 28 158) to 2·66% (N = 25 577) after introducing the new test. Among Venereal Disease Research Laboratory (VDRL)- and CLIA-confirmed results, the false-positive rates were 40·5% (N = 180) and 37·4% (N = 359), respectively (P = 0·5266). Older donors (OR = 1·04; P = 0·0010) and donors with lower education levels (OR = 6·59; P = 0·0029) were associated with a higher risk of positivity for syphilis. CLIA represents an improvement in blood bank serological screening. However, its use in a healthy population appears to result in high rates of false positives. Identifying which characteristics can predict false positives, however, remains a challenge. © 2017 British Blood Transfusion Society.

  3. Relative indexes of cutaneous blood perfusion measured by real-time laser Doppler imaging (LDI) in healthy volunteers.

    Science.gov (United States)

    Seyed Jafari, S Morteza; Schawkat, Megir; Van De Ville, Dimitri; Shafighi, Maziar

    2014-07-01

    We used real-time LDI to study regional variations in microcirculatory perfusion in healthy candidates to establish a new methodology for global perfusion body mapping that is based on intra-individual perfusion index ratios. Our study included 74 (37 female) healthy volunteers aged between 22 and 30 years (mean 24.49). Imaging was performed using a recent microcirculation-imaging camera (EasyLDI) for different body regions of each volunteer. The perfusion values were reported in Arbitrary Perfusion Units (APU). The relative perfusion indexes for each candidate's body region were then obtained by normalization with the perfusion value of the forehead. Basic parameters such as weight, height, and blood pressure were also measured and analyzed. The highest mean perfusion value was reported in the forehead area (259.21APU). Mean perfusion in the measured parts of the body correlated positively with mean forehead value, while there was no significant correlation between forehead blood perfusion values and room temperature, BMI, systolic blood pressure and diastolic blood pressure (p=0.420, 0.623, 0.488, 0.099, respectively). Analysis of the data showed that perfusion indexes were not significantly different between male and female volunteers except for the ventral upper arm area (p=.001). LDI is a non-invasive, fast technique that opens several avenues for clinical applications. The mean perfusion indexes are useful in clinical practice for monitoring patients before and after surgical interventions. Perfusion values can be predicted for different body parts for patients only by taking the forehead perfusion value and using the perfusion index ratios to obtain expected normative perfusion values. Copyright © 2014 Elsevier Inc. All rights reserved.

  4. Occult Hepatitis B virus infection in previously screened, blood donors in Ile-Ife, Nigeria: implications for blood transfusion and stem cell transplantation.

    Science.gov (United States)

    Olotu, Amadin A; Oyelese, Adesola O; Salawu, Lateef; Audu, Rosemary A; Okwuraiwe, Azuka P; Aboderin, Aaron O

    2016-05-05

    Hepatitis B virus (HBV) transmission through blood transfusion is reduced by screening for hepatitis B surface antigen (HBsAg). However this method cannot detect the presence of occult hepatitis B virus infection. This study sought to determine the prevalence of occult hepatitis B virus infection among blood donors in Ile-Ife, Nigeria. For the first time in Nigeria we employed an automated real-time PCR- method to investigate the prevalence of occult HBV in blood donors. Blood donors screened with HBsAg immunochromatographic rapid test kits at the blood transfusion units of two hospitals and found to be negative were recruited into the study. Questionnaires to elicit risk factors for HBV infection were administered and then 10 ml of blood was collected from each donor. Plasma samples obtained from these HBsAg negative blood donors were screened again for HBsAg using an enzyme-linked immunosorbent assay (ELISA) method, and those found negative were screened for the presence of total antibody to the HBV core antigen (anti-HBc) using ELISA method. Those positive to anti-HBc were then tested for HBV DNA, using an automated real-time PCR method. Five hundred and seven blood donors found HBsAg negative by immunochromatographic rapid test kits at both blood transfusion units, were tested for HBsAg using ELISA and 5 (1 %) were HBsAg positive. The 502 found negative were tested for anti-HBc and 354 (70.5 %) were found positive implying previous exposure to HBV and 19 (5.4 %) of the 354 anti-HBc positive had HBV DNA signifying occult HBV infection. No risk factors were found to be associated with the presence of HBV DNA among those who tested positive. Occult HBV infection exists in blood donors in Ile-Ife, Nigeria and the use of HBsAg alone for screening prospective donors will not eliminate the risk of HBV transmission in blood transfusion or stem cell transplantation.

  5. ELEVATED ALANINE AMINOTRANSFERASE (ALT IN BLOOD DONORS: AN ASSESSMENT OF THE MAIN ASSOCIATED CONDITIONS AND ITS RELATIONSHIP TO THE DEVELOPMENT OF HEPATITIS C

    Directory of Open Access Journals (Sweden)

    Fernando Lopes GONÇALES Jr.

    1998-07-01

    Full Text Available The determination of aminotranferases levels is very useful in the diagnosis of hepatopathies. In recent years, an elevated serum ALT level in blood donors has been associated with an increased risk of post-transfusion hepatitis (PTH. The purpose of the study was to research the factors associated with elevated ALT levels in a cohort of voluntary blood donors and to evaluate the relationship between increased ALT levels and the development of hepatitis C (HCV infection. 166 volunteer blood donors with elevated ALT at the time of their first donation were studied. All of the donors were questioned about previous hepatopathies, exposure to hepatitis, exposure to chemicals, use of medication or drugs, sexual behaviour, contact with blood or secretions and their intake of alcohol. Every three months, the serum levels of AST, ALT, alkaline phosphatase, gamma glutamyl transpeptidase, cholesterol, triglyceride and glycemia are assessed over a two year follow-up. The serum thyroid hormone levels as well as the presence of auto-antibodies were also measured. Abdominal ultrasound was performed in all patients with persistently elevated ALT or AST levels. A needle biopsy of liver was performed in 9 donors without definite diagnostic after medical investigation. The presence of anti-HCV antibodies in 116 donors were assayed again the first clinical evaluation. At the end of follow-up period (2 years later 71 donors were tested again for the presence of anti-HCV antibodies. None of donors resulted positive for hepatitis B or hepatitis C markers during the follow-up. Of the 116 donors, 101 (87% had persistently elevated ALT serum levels during the follow-up. Obesity and alcoholism were the principal conditions related to elevated ALT serum levels in 91/101 (90.1% donors. Hypertriglyceridemia, hypercholesterolemia, hypothyroidism and diabetes mellitus also were associated with increased ALT levels. Only 1/101 (0.9% had mild chronic active non A-G viral

  6. [Prevalence of types of hepatitis C virus in Spanish blood donors: results of a state-based multicenter study. Spanish Group for the Study of Blood Donors with Risk of HCV Transmission].

    Science.gov (United States)

    León, P; López, J A; Amela, C; Elola, C; Echevarría, J M

    1999-11-01

    The prevalences established up to the present in Spain for the different types of hepatitis C virus are based on data obtained in populations in which the nature of the population itself may have based the data in favor of certain types of the virus. The study of seropositive blood donors identified through screening of blood donations may provide prevalences closer to the truth among the general population. Typing of genomes in samples from 441 donors was performed using the blood bank generated during the multicenter study performed by the Spanish Study Group of Blood Donors with Risk of Transmission of the Hepatitis C Virus. The antibodies present were typed in the seropositive samples in the above donors and in 337 more in whom a viral genoma was not detected. In total, the infection was typed in 685 donors. On analysis of the results corresponding to 386 donors, whose number and distribution by autonomous communities were previously fixed to represent all of Spain, type 1 was largely the more prevalent (85.5%) followed by types 3 (4.4%), 2 (4.1%), 4 (3.4%) and 5 (0.5%) and by a group of apparent mixed infections which altogether represented 2.1% of the total. Among the donors in whom the genomes were typed, infectious due to the 1b subtype (78% of the 441 samples genotypes) clearly predominated. The participation of the different types of type 1 was significantly greater in those lacking antibodies detectable versus epitopes codified in the NS4 region of the viral genome. This study avoids some bias in sampling which may have affected previous studies and provides data which should more closely approach the real prevalence in the general Spanish population. Thus, it should provide a better base of comparison for any study on the distribution of the types of the hepatitis C virus in selected populations or others performed during tha investigation of outbreaks of hepatitis C virus infection.

  7. Correlation of hepatitis C RNA and serum alanine aminotransferase in hepatitis B and C seronegative healthy blood donors

    Directory of Open Access Journals (Sweden)

    Ali Natasha

    2010-07-01

    Full Text Available Introduction: Historically, serum alanine transaminase (ALT has been used as a surrogate marker in the detection of hepatitis viruses in blood donors. With the availability of newer sensitive technologies for the detection of seroconversion, the value of ALT becomes questionable but continues to be used for this purpose with subsequent discarding of ALT elevated blood units. Objective: The present study aims to evaluate the significance and cost effectiveness of ALT as a surrogate marker for hepatitis C virus infection in healthy asymptomatic blood donors who were serologically negative. Materials and Methods: The study was conducted at clinical laboratory of a tertiary care hospital for a period of one year from November 2006 to October 2007. All donors were screened serologically for hepatitis B, C and HIV I and II, syphilis and malaria and those tested positive were excluded from further evaluation. Gender-wise reference ranges and minimal and markedly raised results for ALT (described respectively as one and two folds increase above reference range were defined and, accordingly, donors were grouped into three. Two hundred seronegative blood donors were randomly selected from all three groups of ALT results and tested for hepatitis C nucleic acid through Amplicor; HCV RNA test. The cost of discarding an ALT -only elevated blood unit was also assessed. During the study period, 25117 subjects donated blood. Eight hundred and Results: seventy two donors (3.4% were positive for one or more serological tests. ALT of all donors ranged from 0-1501 U/L (Mean ± SD; 33.4 ± 25.45U/L. The donors seronegative for all disease markers were 24245 (96.6%. Of these, 21164 (87.2% donors had their ALT within reference range while 2874 (11.8% and 207 (0.8% of donors had minimal and markedly elevated results. Thus, 621 blood bags (red cells, platelets and plasma costing $ 39200.0 were discarded based on ALT results alone. Of 200 seronegative donors evaluated

  8. A study on patterns of co-infections among blood donors at the blood bank of a tertiary care referral teaching hospital in South India

    Directory of Open Access Journals (Sweden)

    Suresh B

    2016-04-01

    Full Text Available Background: Blood serves as a vehicle for transmission of blood-borne pathogens including human immunodeficiency virus (HIV, hepatitis B virus (HBV, hepatitis C virus (HCV, malaria parasite (MP and syphilis. Safe blood and blood products should be transfused to all patients in need for blood transfusion. Material and Methods: All blood donors attending to the blood bank during the period January 2009 to December 2014 were screened for hepatitis B surface antigen (HBsAg, anti HCV antibody, anti HIV-1, 2 antibodies and HIV p24 antigen by using the appropriate enzyme-linked immunosorbent assay (ELISA method and further confirmed using an ELISA kit from a different manufacturer. Malarial antigen testing was done by rapid diagnostic device, which is based on immunochromatographic technique. The rapid plasma reagin (RPR test was used for estimation of syphilis infection and further confirmed by Treponema pallidum haemagglutination assay (TPHA. Results: Of the 41,785 donors who were screened during the study period, 20 (0.05% were reactive for different combination of infections. The various combination of infections seen were as follows; HBV+HCV and HBV+HIV (6/20 each, HIV+HCV (3/20, HIV + syphilis (2/20 and HBV+HIV+HCV, HBV+MP, HBV+syphilis (1/20 each; and HIV+Syphilis constituted for 10% (2/20. Conclusion: A properly conducted donor screening, notification and counseling of permanently deferred donors will help in reducing these co-infection rates.

  9. Profile of Rh, Kell, Duffy, Kidd, and Diego blood group systems among blood donors in the Southwest region of the Paraná state, Southern Brazil.

    Science.gov (United States)

    Zacarias, Joana Maira Valentini; Langer, Ieda Bernadete Volkweis; Visentainer, Jeane Eliete Laguila; Sell, Ana Maria

    2016-12-01

    The aim of this study was to assess the distribution of alleles and genotypes of the blood group systems Rh, Kell, Duffy, Kidd, and Diego in 251 regular blood donors registered in the hemotherapy unit of the Southwestern region of Paraná, Southern Brazil. The frequencies were obtained by direct counting on a spreadsheet program and statistical analyses were conducted in order to compare them with other Brazilian populations using chi-squared with Yates correction on OpenEpi software. The frequencies of RHD* negative, RHCE*c/c and RHCE*e/e were higher than expected for the Caucasian population. A difference was also observed for FY alleles, FY*01/FY*01 genotype and FY*02N.01 -67T/C (GATA Box mutation). Two homozygous individuals were defined as a low frequency phenotype K + k- (KEL*01.01/KEL*01.01) and, for Diego blood group system the rare DI*01 allele was found in ten blood donors, of which one was DI*01/DI* 01 (0.4%). The allele and genotype frequencies of Kidd blood group system were similar to expected to Caucasians. The results showed the direction in which to choose donors, the importance of extended genotyping in adequate blood screening and the existence of rare genotypes in Brazilian regular blood donors. Copyright © 2016 Elsevier Ltd. All rights reserved.

  10. Lack of differences in HIV-1 nef functional domains in infected chinese blood donors at different stages of disease.

    Science.gov (United States)

    Chen, Yue; Caruso, Lori; Shen, Chengli; Wu, Hao; Zhou, Yushen; Gupta, Phalguni

    2007-09-01

    HIV-1 nef regions were amplified by polymerase chain reaction and sequenced from DNA samples of five asymptomatic subjects and five AIDS patients from a cohort of HIV-1-infected Chinese plasma and blood donors. Sequence analysis revealed that regardless of the stage of disease, each patient's HIV-1 nef sequences belonged to the clade B' subtype. Although there are some differences between the sequences from different patients, no significant differences have been detected in nef nucleotide sequences or functional motifs in the deduced amino acid sequences from patients at different stages of the disease. Furthermore, the predicted binding motifs of HLA-A2 and HLA-A11 were highly conserved among patient nef sequences. These results will contribute to a better understanding of the pathogenesis of circulating HIV-1 in infected Chinese former blood donors and may have important implications in developing an epitope-based vaccine suitable for Chinese blood donors.

  11. Revaluing donor and recipient bodies in the globalised blood economy: transitions in public policy on blood safety in the United Kingdom.

    Science.gov (United States)

    Busby, Helen; Kent, Julie; Farrell, Anne-Maree

    2014-01-01

    The clinical use of blood has a long history, but its apparent stability belies the complexity of contemporary practices in this field. In this article, we explore how the production, supply and deployment of blood products are socially mediated, drawing on theoretical perspectives from recent work on 'tissue economies'. We highlight the ways in which safety threats in the form of infections that might be transmitted through blood and plasma impact on this tissue economy and how these have led to a revaluation of donor bodies and restructuring of blood economies. Specifically, we consider these themes in relation to the management of recent threats to blood safety in the United Kingdom. We show that the tension between securing the supply of blood and its products and ensuring its safety may give rise to ethical concerns and reshape relations between donor and recipient bodies.

  12. Prevalence of anti-A and anti-B hemolysis among blood group O donors in Lagos.

    Science.gov (United States)

    Oyedeji, O A; Adeyemo, T A; Ogbenna, A A; Akanmu, A S

    2015-01-01

    Group O donor blood is more readily available and is frequently used as universal red cell donor in our environment. The presence of hemolysins in the donors may however lead to hemolysis in the recipients. Attempts have been made to study the prevalence of hemolysins in various populations with results from our environment showing wide variation (20-80%). To determine the prevalence and titer of anti-A and anti B hemolysins among blood donors at the Lagos University Teaching Hospital and compare results with that obtained elsewhere. Determine if the practice of transfusion of group O blood to nongroup O recipients is permissible in this environment. Test for hemolysis was done using the standard tube method. Samples positive for hemolysis were then scored and titrated with the titers read visually and photometrically at 540 nm. Three hundred and fifty blood group O donors with age range 18-58 years and median age of 28 ΁ 8.4 years were enrolled in the study. The overall prevalence of anti-A and/or anti-B hemolysins obtained was 30.3%. Prevalence of anti-A and anti-B hemolysins only was 15.4% and 5.1% respectively whereas both anti-A and anti-B hemolysins were present in 9.7% donor samples. Though anti-A hemolysins were more prevalent than anti-B hemolysins, anti-B hemolysins had higher mean visual (6:7) and spectrophotometric titers (81:101). A visual titer of 8 and above which is considered significant was seen in 18.6% of donor samples. Anti-A and anti-B hemolysins exist in significant frequencies and titers among blood group O donors in Lagos. It is recommended that the use of group O donor blood for recipients who are non-O be discouraged. Clinical studies to determine the frequency and severity of hemolysis in non-group O recipients of blood group O are required.

  13. Prevalence of hepatitis B surface antigen & its subtypes in high risk group subjects & voluntary blood donors in Bombay.

    Science.gov (United States)

    Elavia, A J; Banker, D D

    1991-09-01

    HBsAg positive subjects belonging to high risk groups and voluntary blood donors were analysed for prevalence of HBsAg among various groups of subjects for ascertaining the carrier status among the voluntary blood donors, HBsAg subtype distribution, and association of HBsAg with blood groups and caste or religion. The prevalence of HBsAg varied from 2.02 per cent in voluntary blood donors to 58.38 per cent in patients of acute viral hepatitis. 70.5 per cent subjects had subtype 'ay' while 23.9 per cent of the subjects had subtype 'ad'. We also found compound 'ady' subtype in 5.6 per cent of our subjects. HBsAg/adr, a subtype not usually prevalent in India, was found in 30 of the 90 'ad' sera. Co-occurrence of HBsAg and anti-HBs was noted in 9 subjects. Homotypic anti-HBs was found to occur together mainly in voluntary blood donors, while heterotypic anti-HBs was found to occur together mainly multi-transfused patients. There was no significant correlation between HBsAg and blood group antigens and a relatively higher incidence of HBsAg among the Jain community was observed.

  14. Successful second transplantation from haploidentical donor for graft failure following unrelated cord blood cell transplantation or mismatched related transplantation: 2cases report

    Institute of Scientific and Technical Information of China (English)

    XU Lan-ping; HUANG Xiao-jun

    2006-01-01

    @@ Cord blood transplantation (CBT) from unrelated donors has increasingly been performed worldwide during the last decade. The immaturity of lymphocytes in cord blood permits HLA-mismatching between donors and recipients and reduces the severity of graft-versus-host disease (GVHD).However, the relatively small dose of the cord blood nucleated cells is associated with a high frequency of engraftment failure.1-5 But re-transplantation with stem cells from the original donor is impossible.

  15. Burden of hepatitis C virus infection and its genotypes among the blood donors at Tirupati, Andhra Pradesh

    Directory of Open Access Journals (Sweden)

    Verma A

    2015-10-01

    Full Text Available Background: Safe blood donation remains a challenge in resource limited countries. False positive serological tests lead to wastage of large number of blood units. Nucleic acid amplification test (NAAT technology has greatly enhanced the accuracy in identification of transfusions transmitted infections. Methods: The present study was undertaken to study the seroprevalence of hepatitis C virus (HCV and asses the concordance between seropositivity with the presence of HCV ribonucleic acid (RNA and to know the distribution pattern of HCV genotypes in healthy blood donors. Results: Among the 9287 donors screened (88.3% males, 7153 (77% were voluntary donors while 2134 (23% were replacement donors. Among blood donors, 27 (0.3% samples tested HCV seropositive. Among 27 anti-HCV positive samples only 11 (41% were found positive for HCV RNA. Among the 5 samples subjected to sequencing, three were found to be genotype 1a while two were genotype 3a. Conclusions: Our observations suggest that implementing NAAT test for HCV screening will be helpful in minimizing false-positive test results in the Indian setting.

  16. [Syphilis detection using ELISA and VDRL tests on blood donors at the blood center of Guarapuava, State of Paraná].

    Science.gov (United States)

    Oliveira, Viviane Matoso de; Verdasca, Izabel Cristina; Monteiro, Marta Chagas

    2008-01-01

    The aim of this study was to evaluate the syphilis seroprevalence among 5,752 blood donors who were attended at the blood center of Guarapuava, State of Paraná, in 2006. The seropositivity rates were 2.1% for enzyme Linked Immuno Sorbent Assay and 0.2% for Veneral Disease Research Laboratory, thus showing low prevalence of syphilis among the individuals who came to this blood bank.

  17. Evaluation of Two Different Methods for Hemoglobin Screening of Blood Donors to Use in Mobile Blood Collection Team

    Directory of Open Access Journals (Sweden)

    Soner Yilmaz

    2014-10-01

    Full Text Available AIM: The aim of the study is to compared two point-of care hemoglobin concentration measuring devices with laboratory measurements to determine their accuracy in mobile blood collection conditions. This two devices were the Pronto-7 (Masimo Corp., Irvine,CA pulse co-oximeter and HemoCue Hb 201+ uses spectrophotometric method. METHODS: Blood samples were collected from 152 individuals. The samples were tested for total hemoglobin measurement by three different methods: noninvaziv pulse co-oximeter device Pronto-7, finger-stick blood sample on an automated spectrophotometric device HemoCue and venous samples on an automated hemotology analyzer (reference device RESULTS: According to the venous samples (mean Hb value is 14.6 g/dl, the mean hemoglobin levels were detected slightly higher in the fingerstick samples (mean Hb value is 15.1 g/dl and slightly lower in the pulse co-oximetry (mean Hb value is 14.1 g/dl. The sensivity of HemoCue, Pronto-7 was 97.7%, 81.6% and their specifity was 31.2% and %43.7, respectively. The positive predictive values (PPV of HemoCue and Pronto-7 were high (92.3% and 92.5%, respectively but both of their negative predictive values (NPV were low (62.5% and 21.8%, respectively. Of blood donors, 88.4% (138/152 percent were correctly classified with HemoCue and 78.2% were with Pronto-7. COCLUSION: Taking automated laboratory hemoglobin measurement as a reference, Pronto-7 gave lower readings for Hb value and was less accurate than the Hemo-Cue system [TAF Prev Med Bull 2014; 13(5.000: 391-394

  18. Evaluation of the baseline Widal titres in healthy blood donors of Uttarakhand

    Directory of Open Access Journals (Sweden)

    Garima Mittal

    2014-09-01

    Full Text Available Introduction: The interpretation of Widal test depends upon the baseline titre which is prevalent amongst healthy individuals in a particular geographical area. Aim and Objective: The aim of this study was to determine the baseline Widal titre of the apparently healthy population of a tertiary care centre of Uttarakhand. Material and Methods: Blood samples were collected from healthy blood donors (n=500 of the age group of 18-60 years, of both the sexes, who attended our blood bank from September 2013 to November 2013 and were analyzed for the presence of Salmonella antibodies by carrying out the Widal tube agglutination test. Results: Of the 500 serum samples which were tested, 255 (51% serum samples were positive for agglutinins (≥ 1:20 and 245 were negative. The most frequently recorded titre of the reactive sera was 1:40 for the anti-O antibodies and 1:80 for the anti-H antibodies for Salmonella enterica serotype typhi and this was the baseline titre. While the baseline titre of the ‘H’ agglutinins of Salmonella enterica serotype paratyphi A was 1:20 and of paratyphi B was also1:20. Conclusions: Based on the results of our study, it has been recommended that the significant titre of 1:80 for the anti-O antibodies and of 1:160 for the anti-H antibodies may be considered as diagnostic for enteric fever in the region of Uttarakhand, India.

  19. Human T-Cell Lymphotropic Virus Types 1 and 2 Seropositivity among Blood Donors at Mbarara Regional Blood Bank, South Western Uganda.

    Science.gov (United States)

    Uchenna Tweteise, Patience; Natukunda, Bernard; Bazira, Joel

    2016-01-01

    Background. The human T-cell lymphotropic virus types 1 and 2 (HTLV 1/2) are retroviruses associated with different pathologies. HTLV-1 causes adult T-cell leukemia/lymphoma (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP); HTLV-2 is not clearly associated with a known clinical disease. Both viruses may be transmitted by whole blood transfusion, from mother to child predominantly through breastfeeding, and by sexual contact. Presently, none of the regional blood banks in Uganda perform routine pretransfusion screening for HTLV. The aim of this study was to determine the prevalence of anti-human T-cell lymphotropic virus types 1/2 (HTLV-1/2) antibodies among blood donors at Mbarara Regional Blood Bank in South Western Uganda. A cross-sectional study was conducted between June 2014 and September 2014. Methodology. Consecutive blood samples of 368 blood donors were screened for anti-HTLV-1/2 antibodies using an enzyme linked immunosorbent assay (ELISA). Samples reactive on a first HTLV-1/2 ELISA were further retested in duplicate using the same ELISA. Of the three hundred and sixty-eight blood donors (229 (62.2%) males and 139 (37.8%) females), only two male donors aged 20 and 21 years were HTLV-1/2 seropositive, representing a prevalence of 0.54%. Conclusion. HTLV-1/2 prevalence is low among blood donors at Mbarara Regional Blood Bank. Studies among other categories of people at risk for HTLV 1/2 infection should be carried out.

  20. Human T-Cell Lymphotropic Virus Types 1 and 2 Seropositivity among Blood Donors at Mbarara Regional Blood Bank, South Western Uganda

    Directory of Open Access Journals (Sweden)

    Patience Uchenna Tweteise

    2016-01-01

    Full Text Available Background. The human T-cell lymphotropic virus types 1 and 2 (HTLV 1/2 are retroviruses associated with different pathologies. HTLV-1 causes adult T-cell leukemia/lymphoma (ATL and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP; HTLV-2 is not clearly associated with a known clinical disease. Both viruses may be transmitted by whole blood transfusion, from mother to child predominantly through breastfeeding, and by sexual contact. Presently, none of the regional blood banks in Uganda perform routine pretransfusion screening for HTLV. The aim of this study was to determine the prevalence of anti-human T-cell lymphotropic virus types 1/2 (HTLV-1/2 antibodies among blood donors at Mbarara Regional Blood Bank in South Western Uganda. A cross-sectional study was conducted between June 2014 and September 2014. Methodology. Consecutive blood samples of 368 blood donors were screened for anti-HTLV-1/2 antibodies using an enzyme linked immunosorbent assay (ELISA. Samples reactive on a first HTLV-1/2 ELISA were further retested in duplicate using the same ELISA. Of the three hundred and sixty-eight blood donors (229 (62.2% males and 139 (37.8% females, only two male donors aged 20 and 21 years were HTLV-1/2 seropositive, representing a prevalence of 0.54%. Conclusion. HTLV-1/2 prevalence is low among blood donors at Mbarara Regional Blood Bank. Studies among other categories of people at risk for HTLV 1/2 infection should be carried out.

  1. Markers infectious agent in the cord blood samples public register of donors

    Directory of Open Access Journals (Sweden)

    A. B. Smoljaninov

    2014-01-01

    Full Text Available Objective. To evaluate the distribution of markers of infectious agents in umbilical cord blood samples Pokrovskij public stem cell bank donor registry for five years (2009 – 2013.Materials and Methods. 3533 plasma samples were investigatedafter selection during cord blood processing procedure for allogeneic use in Pokrovskij stem cell bank. All plasma samples were investigated in accordance with the Order of the Ministry of Health № 325 – 2003 by enzymelinked immunoassay method. In addition, during the period from November 2011 to December 2013 1030 plasma samples of umbilical cord blood were examined for the presence of HCV RNA, the RNA of HIV and HBV DNA.Results. Markers of the agents above have not been found in the plasma of 481 samples (13.6%. During the described period, no significant change in the share of samples containing antibodies to cytomegalovirus and toxoplasmosis (cytomegalovirus – 1978 samples (56%, Toxoplasma gondii – 112 samples (3.2%, 825 samples (23.4% cytomegalovirus and Toxoplasma gondii simultaneously were registered. 137 samples (3.9% were subjected to utilization in connection with detection of antibodies to HbcorAg – 116 samples (3.3%, antibodies to HCV – five samples (0.14%, and antibodies to Treponema pallidum – 16 samples (0.45%.Conclusion. The introduction of an additional method of polymerase chain reaction for the detection of nucleic acids of hepatitis viruses B, C, human immunodeficiency virus, along with study of cord blood samples by enzyme-linked immunoassay improve the quality of the control of the transmission of blood-borne infections.

  2. A novel hepatitis B virus subgenotype, D7, in Tunisian blood donors.

    Science.gov (United States)

    Meldal, Birgit H M; Moula, Najet Mojaat; Barnes, If H A; Boukef, Kamel; Allain, Jean-Pierre

    2009-07-01

    Tunisia is a medium-level epidemic country for hepatitis B virus (HBV). This study characterizes, for the first time, full genome HBV strains from Tunisia. Viral load quantification and phylogenetic analyses of full genome or pre-S/S sequences were performed on 196 hepatitis B surface antigen (HBsAg)-positive plasma samples from Tunisian blood donors. The median viral load was 64.65 IU ml(-1) (rangesubgenotype D7, 41 strains clustered in subgenotype D1, seven strains in subgenotype A2 and one strain in genotype C. The novel subgenotype D7 was defined by maximum Bayesian posterior probability, a genetic divergence from other HBV/D subgenotypes by >4% and a stronger HBV/E signal in the X to core genes than subgenotype D1. In conclusion, HBV/D is dominant in asymptomatic Tunisian HBsAg carriers and a novel subgenotype, D7, was the most common subgenotype found in this population.

  3. Various functions of PBMC from colon cancer patients are not decreased compared to healthy blood donors

    DEFF Research Database (Denmark)

    Afzelius, P; Nielsen, Hans Jørgen

    1997-01-01

    -2 and its receptor proteins in T helper cells. The proliferative responses and IL-2 synthesis of PBMC have earlier been shown to be reduced in patients with colon cancer. Recently immune modulating agents have been demonstrated to increase the proliferative response of PBMC in vitro, probably...... by inhibition of adenylate cyclase activity and induction of IL-2 mRNA expression. We have therefore studied the proliferative responses of PBMC from colon cancer patients to PWM and tested the effect of immune modulating agents, such as Serotonin, Sumatriptan, and Buspirone on these PBMC. We found...... no difference in levels of intracellular cAMP, IL-2 mRNA expression, IL-2R mRNA expression, or proliferative responses of PBMC from colon cancer patients compared to healthy blood donors. There was no effect of the immune modulating agents on PBMC from colon cancer patients....

  4. Plasma selenium levels in healthy blood bank donors in the central-eastern part of Belgium.

    Science.gov (United States)

    Van Cauwenbergh, Rudy; Robberecht, Harry; Van Vlaslaer, Veerle; De Smet, Annie; Emonds, Marie-Paule; Hermans, Nina

    2007-01-01

    Graphite furnace atomic absorption spectrometry, with Zeeman background correction and after improved matrix modification, was used to measure the plasma selenium content of healthy blood bank donors in the central part of Belgium. The mean plasma selenium concentration of 80 men and 80 women was 79.7+/-4.4ng/mL with a range of 55.0-117.4ng/mL. There was no gender difference observed. Plasma selenium level was significantly highest for the adult group, aged 45-64 years, compared to the others, except the young adults (18-24 years). The mean plasma selenium concentration measured corresponded well with literature data for Belgium. The obtained values were found to be in the medium range, compared with recent literature values for the European countries.

  5. Sideropenia sem anemia em doadores de sangue do Hemocentro do Amazonas - Hemoam Sideropenia without anemia in blood donors of the Amazon Blood Bank - Hemoam

    Directory of Open Access Journals (Sweden)

    Leny N. M. Passos

    2005-03-01

    Full Text Available Uma doaç��o de aproximadamente 475mL de sangue depleta em média 242 ± 17 mg de ferro do doador, o que pode ter conseqüências variáveis em suas reservas e na sua saúde. Tivemos por objetivo avaliar se doadores de sangue do Hemocentro do Amazonas - Hemoam desenvolvem sideropenia sem anemia após doações consecutivas. A ferritina sérica foi medida em 528 doadores de sangue, do sexo masculino, com idade entre 18 a 61 anos, divididos em 313 doadores de repetição, com 4 ou mais doações regulares, e 215 primodoadores, que compareceram ao Hemocentro do Amazonas no período de setembro de 2001 a junho de 2002. Depleção do depósito de ferro, definida por níveis de ferritina menores de 20 ng/L, foi encontrada em 7,4% [16/215] dos primodoadores e em 48,6% [152/313] dos doadores de repetição. Utilizando-se de um critério mais rigoroso, como valores de ferritina A blood donation of 475 mL could deplete 242 ± 17 mg of iron from blood donors. The objective of this report is to evaluate if blood donors could develop sideropenia without anemia after several donations. Serum levels of ferritin were measured in 528 male blood donors, with ages ranging from 18 to 61 years old. A total of 313 of them had made 4 or more donations and 215 of them were first time donors. They donated blood in the Hemocentro do Amazonas - Hemoam, from September 2001 to June 2002. Deletion of iron stores characterized by serum ferritin levels of less than 20 ng/L was found in 7.4% (16/215 of first time donors, and in 48.6% (152/313 of multiple donors. With more stringent criteria of ferritin values less than 12 ng/L, 3.7% (8/215 of first-time donors as opposed to 24.9% (78/313 of multiple donors showed severe depletions. We concluded that multiple donors, after more than 5 repeated donations, are at risk of depleted iron and ferritin levels. It is important to implant protocols of iron supplementation for these donors to avoid damage to their health and

  6. Behavior assessment of blood donors facing the risk of HIV infection, Abidjan, Cotê D'Ivoire, 2001-2002.

    Science.gov (United States)

    Minga, Albert K; Huët, Charlotte; Dohoun, Lambert; Abo, Yao; Bonard, Dominique; Gourvellec, G; Coulibaly, Ali; Konaté, Seidou; Dabis, Francois; Salamon, Roger

    2005-04-15

    Despite precautions taken to guarantee blood safety, in the National Blood Transfusion Center (CNTS) of Abidjan, about 30 regular blood donors are detected with HIV seroconversion each year, two-thirds of them men. A survey through face-to-face interviews was carried out at the CNTS of Abidjan from September 2001 to March 2002 among HIV-positive and HIV-negative regular blood donors, informed about their serologic status. HIV-negative regular blood donors informed about their serologic status since a median time of 67 months (n = 50) disclosed more risky behaviors such as multiple sexual partners (68%) than HIV-positive blood donors informed about their status (n = 112) since a median time of 35 months (41%) (P < 0.001). Condoms were systematically used by 17% of HIV-negative blood donors and 55% of HIV-positive blood donors (P < 0.001). Enhanced counseling and awareness could reduce in the future the number of cases of seroconversion among regular blood donors and improve their subsequent behavior. Blood donors who have unprotected sex with partners of unknown HIV serologic status and especially with casual partners are strongly exposed to HIV transmission and should be discouraged to continue giving blood, after adequate counseling.

  7. Time Trend of the Prevalence of Hepatitis E Antibodies among Farmers and Blood Donors: A Potential Zoonosis in Denmark

    Science.gov (United States)

    Christensen, Peer B.; Engle, Ronald E.; Hjort, Charlotte; Homburg, Keld M.; Vach, Werner; Georgsen, Jørgen; Purcell, Robert H.

    2009-01-01

    Background Antibody to hepatitis E virus (anti-HEV) is prevalent in Western countries, where clinical hepatitis E is rarely reported. The aim of this study was to determine the prevalence of anti-HEV among Danish blood donors and Danish farmers. In addition, we compared the prevalence among 2 sets of serum samples obtained from blood donors 20 years apart. Methods Samples from 291 Danish farmers and 169 blood donors that were collected in 1983 and samples from 461 blood donors that were collected in 2003 were tested for anti-HEV. Relevant information on HEV exposure was collected by self-administered questionnaire. Results Anti-HEV testing was performed on samples after 20 years of storage at −20°C. The prevalence of anti-HEV was 50.4% among farmers and 32.9% among donors in 1983 and 20.6% among donors in 2003 (P < .05). Presence of anti-HEV was significantly correlated with increasing age in all 3 groups (P < .05). Among donors who had serum samples obtained in 2003, age, contact with horses, and the presence of antibody to hepatitis A virus were associated with the presence of anti-HEV in multivariate analysis. Among farmers, only age was independently associated with the presence of anti-HEV. Conclusion Anti-HEV was highly prevalent among Danes but has decreased in prevalence over the past 50 years. Our study supports the hypothesis that HEV infection in Denmark may be an asymptomatic zoonotic infection. PMID:18781880

  8. Role of Medical Social Workers in Management of Anxiety and Stress Among Blood Donors with Transfusion Transmissible Infections

    Directory of Open Access Journals (Sweden)

    Umakanth Siromani

    2014-02-01

    Full Text Available Blood transfusion is a life-saving intervention and millions of lives are saved each year globally through this procedure. Unsafe transfusion practices put millions of people at risk of transfusion-transmissible infections (TTIs and it is mandatory to test the donated blood for blood borne infectious diseases. This is an alarming situation requiring immediate action in appropriate counseling of donors before and after testing of their blood. It is really a challenge for blood banks and motivators if a blood donor is positive for infectious diseases. What is the role of blood bank in helping or guiding the donors to overcome their anxiety and stress? How a medical social worker could psychologically support and guide them to act as cause ambassadors for voluntary blood donation? Guidance and counseling would help them to live positively. Health education, compassionate care and teaching coping mechanisms would encourage them in overcoming their stress and anxiety. [Natl J Med Res 2014; 4(1.000: 87-88

  9. High prevalence of antibodies against polyomavirus WU, polyomavirus KI, and human bocavirus in German blood donors

    Directory of Open Access Journals (Sweden)

    Opitz Andreas

    2010-07-01

    Full Text Available Abstract Background DNA of the polyomaviruses WU (WUPyV and KI (KIPyV and of human bocavirus (HBoV has been detected with varying frequency in respiratory tract samples of children. However, only little is known about the humoral immune response against these viruses. Our aim was to establish virus-specific serological assays and to determine the prevalence of immunoglobulin G (IgG against these three viruses in the general population. Methods The capsid proteins VP1 of WUPyV and KIPyV and VP2 of HBoV were cloned into baculovirus vectors and expressed in Sf9 insect cells. IgG antibodies against WUPyV VP1, KIPyV VP1, and HBoV VP2 were determined by immunofluorescence assays in 100 plasma samples of blood donors. Results The median age of the blood donors was 31 years (range 20 - 66 yrs, 52% were male. 89% of the samples were positive for WUPyV IgG (median age 31 yrs, 49.4% male, 67% were positive for KIPyV IgG (median age 32 yrs, 46.3% male, and 76% were positive for HBoV IgG (median age 32 yrs, 51.3% male. For WUPyV and HBoV, there were no significant differences of the seropositivity rates with respect to age groups or gender. For KIPyV, the seropositivity rate increased significantly from 59% in the age group 20 - 29 years to 100% in the age group > 50 years. Conclusions High prevalences of antibodies against WUPyV, KIPyV, and HBoV were found in plasma samples of healthy adults. The results indicate that primary infection with these viruses occurs during childhood or youth. For KIPyV, the seropositivity appears to increase further during adulthood.

  10. [Serological screening for Leishmania infantum in asymptomatic blood donors and HIV+ patients living in an endemic area].

    Science.gov (United States)

    Scarlata, Francesco; Li Vecchi, Valentina; Abbadessa, Vincenzo; Giordano, Salvatore; Infurnari, Laura; Saporito, Laura; Mancuso, Salvatrice; Occhipinti, Francesco; Vitale, Fabrizio; Reale, Stefano; Titone, Lucina

    2008-03-01

    Visceral leishmaniasis (VL) is endemic in Sicily (48 new cases in 2004, of which nine were in Agrigento). In southern Europe between 25-70 per cent of adult VL cases are related to HIV infection. The HIV cases have a high risk (1.5-9%) of developing VL either as a new infection or as the revival of a latent infection. We therefore carried out serologic screening to detect antibodies against L. infantum by IFAT in 1449 blood donors in Agrigento and the surrounding area (May-December 2005) and in 120 HIV+ in western Sicily, all of whom were asymptomatic and had no history of VL. L. DNA was assessed by nested PCR in blood samples of some seropositive donors. Of the 1449 blood donors, 11 (0.75%) were positive by IFAT and three of them were also positive in PCR. L. infantum seropositivity is most probably the expression of recent infection because the clearance of serum antibodies is rather fast (6-12 months) after VL. This is why blood donation by Leishmania seropositive donors, whether positive or negative by PCR, could constitute an infection risk especially for immunosuppressed recipients, who should receive deleukocyted blood. Moreover it could be useful to monitor HIV/Leishmania coinfection cases to avoid the risk of slatentization of L. infection when CD4+ levels are very low.

  11. High prevalence of anti-hepatitis E virus antibodies among blood donors in central Italy, February to March 2014.

    Science.gov (United States)

    Lucarelli, Claudia; Spada, Enea; Taliani, Gloria; Chionne, Paola; Madonna, Elisabetta; Marcantonio, Cinzia; Pezzotti, Patrizio; Bruni, Roberto; La Rosa, Giuseppina; Pisani, Giulio; Dell'Orso, Luigi; Ragone, Katia; Tomei, Carla; Ciccaglione, Anna Rita

    2016-07-28

    Prevalence of anti-hepatitis E virus (HEV) antibodies is highly variable in developed countries, which seems partly due to differences in assay sensitivity. Using validated sensitive assays, we tested 313 blood donors attending a hospital transfusion unit in central Italy in January and February 2014 for anti-HEV IgG and IgM and HEV RNA. Data on HEV exposure were collected from all donors. Overall anti-HEV IgG prevalence was 49% (153/313). Eating raw dried pig-liver sausage was the only independent predictor of HEV infection (adjusted prevalence rate ratio = 2.14; 95% confidence interval: 1.23-3.74). Three donors were positive for either anti-HEV IgM (n = 2; 0.6%) or HEV RNA (n = 2; 0.6%); they were completely asymptomatic, without alanine aminotransferase (ALT) abnormalities. Of the two HEV RNA-positive donors (both harbouring genotype 3), one was anti-HEV IgG- and IgM-positive, the other was anti-HEV IgG- and IgM-negative. The third donor was positive for anti-HEV IgG and IgM but HEV RNA-negative. HEV infection is therefore hyperendemic among blood donors (80% men 18-64 years-old) from central Italy and associated with local dietary habits. Nearly 1% of donors have acute or recent infection, implying potential transmission to blood recipients. Neither ALT nor anti-HEV IgM testing seems useful to prevent transfusion-transmitted HEV infection.

  12. A case of nearly mistaken AB para-Bombay blood group donor transplanted to a group 'O' recipient.

    Science.gov (United States)

    Townamchai, Natavudh; Watanaboonyongcharoen, Phandee; Chancharoenthana, Wiwat; Avihingsanon, Yingyos

    2014-10-31

    Unintentional ABO mismatch kidney transplantation can cause detrimental hyperacute rejection. We report the first successful ABO incompatible kidney transplantation from an AB para-Bombay donor to O recipient. At the initial evaluation, the donor's ABO type was discordance on the cell typing and serum typing, which typed to be 'O' as cell typing and 'AB' as serum typing. At the second investigation, it was confirmed that the donor had a unique, rare but not uncommon blood type AB para-Bombay which was incompatible with the recipient's blood group. The kidney transplantation was successfully performed by an ABO incompatible preconditioning, double filtration plasmapheresis (DFPP) and rituximab. The serum creatinine at 12 months post-transplantation was 1.3 mg/dL. The pathology of the kidney biopsy showed no signs of rejection.

  13. Perioperative use of allogenic blood components in live-related donor orthotopic liver transplantation: A cross sectional study.

    Science.gov (United States)

    Pandey, Prashant; Tiwari, Aseem K; Sharma, Jyoti; Srivastava, Divyajyoti; Dixit, Surbhi; Raina, Vimarsh

    2013-01-01

    In spite of many improvements that have reduced the blood component requirements, substantial numbers of transfusions are still needed in liver transplantation. The objective of the present study was to analyze the perioperative usage of allogenic blood components and predict the preoperative factors as predictors of red cell transfusion in live-related donor liver transplant recipients. The retrospective data on utilization of allogenic blood components were analyzed for a total of 150 liver transplant procedures. The data on utilization of blood components during surgery and till 48 hours of ICU stay was collected from the blood bank record and hospital information system (HIS). Red cell concentrate was commonest blood component used in liver transplant recipient and most of the transfusion took place during surgery. During intraoperative period 92.7% (N = 139) of the cases utilized red cell components with the median number of five whereas in postoperative period only 38% (N = 57) of patients received blood with the median number of one. This study demonstrates that the preoperative hemoglobin and platelet count are the predictors of utilization of red cell concentrates during surgery. There were a total of 11 (7.3%) recipients who didn't receive allogeneic blood transfusion in any form. Utilization of blood components was negligible among organ donors. Our study demonstrates the pattern and predictors of usage of allogeneic blood components in liver transplant recipients at a tertiary healthcare center in India.

  14. Co-infection rate of HIV, HBV and Syphilis among HCV seropositive identified blood donors in Kathmandu, Nepal

    Directory of Open Access Journals (Sweden)

    Ashish Chandra Shrestha

    2012-02-01

    Full Text Available Background: HIV, HBV, Syphilis and HCV share common modes of transmission. Objective: The study was aimed to determine the co-infection rate of HIV, HBV and Syphilis among HCV seropositive identified blood donors. Methods: The study was conducted on blood samples screened as HCV seropositive at Nepal Red Cross Society, Central Blood Transfusion Service, Kathmandu, Nepal. HCV seropositive samples were further tested for HIV, HBV and Syphilis. Results: Eight co-infections were observed in 139 HCV seropositives with total co-infection rate of 5.75% (95% CI = 2.52-11.03. Conclusion: Co-infection of HIV, HBV and Syphilis with HCV is prevalent in the healthy looking blood donors of Kathmandu, Nepal.

  15. 病毒核酸检测在献血者保留中的应用%The application of nucleic acid testing in keeping blood donor

    Institute of Scientific and Technical Information of China (English)

    李新建

    2013-01-01

    Objective To find out the situation of Hepatitis B virus and Hepatitis C virus in Anyang city by analyzing false positive on ELISA method,and to assess blood samples of voluntary blood donors using detecting nucleic acid of HBV and HCV.Methods ELISA makes blood samples of 50 267 voluntary blood donors,and Real Time PCR tests positive specimens on HBsAg and antibody to HCV,and then blood donors whose results were single positive ELISA and negative NAT would be tracked after 6 months.Results The unmatch rate between two ELISA products in HBV and HCV tests was 46.7%.There were 249 specimens which showed positive in either of two ELISA tests but negative in PCR,and 103 samples that perform positive data in both ELISA products but negative PCR results.The x2 was 114.154 (P<0.01) between single and both positive HBV results,and this statistical item was 61.109 (P<0.01) in HCV comparison.83 of 91 tracked men had ELISA (-) NAT (-) results.Conclusion Real Time PCR could distinguish effectively false positive ELISA results of voluntary blood donors.We should apply Real Time PCR to our volunteers for keeping current blood donors and improving regular ones.%目的 了解安阳地区献血人群中,乙型肝炎病毒(HBV)和丙型肝炎病毒(HCV)用酶联免疫吸附法(ELASL)检测的假阳性情况,对无偿献血人群的血标本进行HBV和HCV核酸检测(NAT)的可行性评估.方法 对无偿献血者血液样本50 267份开展ELISA检测,对乙型肝炎病毒表面抗原(HBsAg)和抗丙型肝炎病毒抗体(抗-HCV)阳性标本开展实时荧光定量PCR检测,并对ELISA单试剂(+)NAT(-)献血者6个月后进行追踪检测.结果 两个项目两种ELISA试剂检测结果不相符率达46.7%,ELISA检测方法单试剂阳性者PCR呈阴性者249份,EIISA检测方法双试剂阳性者PCR呈阴性者103份.HBV项目单试剂阳性组与双试剂阳性组比较,差异有统计学意义(x2 =114.154,P<0.01);HCV项目单试剂阳性组与双试剂阳性组比

  16. Influence of social activity on regional cerebral blood flow and mental function in the normal aged volunteers

    Energy Technology Data Exchange (ETDEWEB)

    Kobayashi, Shotai; Yamaguchi, Shuhei; Katsube, Tomoko; Kitani, Kohaku; Okada, Masanori (Shimane Medical University (Japan))

    1983-12-01

    The infuence of social activity on regional cerebral blood flow (rCBF) and mental function was studied by Xe133 inhalation method in normal aged volunteers. Subjects: The first group consisted of 33 aged volunteers living in nursing home and exposed to little social stimuli. There were 15 males (mean age of 77 years) and 18 females (77 years). The second group consisted of 49 aged community volunteers who were confirmed socially active. There were 25 males (76 years) and 24 females (72 years). All subjects were healthy persons without a past history of cerebral diseases and lung diseases. There were no difference in blood pressure and hematocrit between the two groups. The rCBF was measured by 16-ch-Novo-cerebrograph. Verbal intelligence was evaluated by the Hasegawa Simple Intelligence Scale for Aged. Performance intelligence was evaluated with the Kohs' Block Design Test. The mean rCBF in group I showed significantly lower value than that of group II, especially in the frontotemporal region. The performance intelligence was decreased in group I. However, there were no significant difference in the verbal intelligence between the two groups. 2) The aging effect on rCBF and intelligences was more prominent in group II. 3) In males, hemispheric rCBF of group I decreased bilaterally associated with the decrease of both intelligences. While the left hemispheric rCBF in females was relatively preserved as well as the preservation of verbal intelligence. These results indicate that the social environmental factors may have significant influence to aging of the brain especially in the males.

  17. [Identification of blood donors capable of transmitting P. falciparum. Use of homologous antigenic preparations ready for use].

    Science.gov (United States)

    Deroff, P; Reguer, M; Simitzis, A M; Boudon, A; Saleun, J P

    1982-09-01

    The smears of P. falciparum parasited blood ready to be employed, that we have tested in indirect immunofluorescence to detect blood donors who can be at the origin of post transfusional paludism, seem to offer a very important progress to us for the improvement of transfusion security. Their routine use can be recommended because their easy way of using allows their adoption by very many transfusion centers.

  18. [Profile of HIV infected patients among blood donors in Abidjan, Côte d'Ivoire (1992-1999)].

    Science.gov (United States)

    Minga, A K; Huët, C; Coulibaly, I; Abo, Y; Dohoun, L; Bonard, D; Gourvellec, G; Coulibaly, Y; Konaté, S; Dabis, F; Bondurand, A; Salamon, R

    2005-06-01

    The FonSIDA is a private clinic created in 1992 within the premises of the National Blood Transfusion Center of Abidjan (CNTS), the largest city in Côte d'Ivoire. It provides medical and psychological follow-up for blood donors which are diagnosed as HIV-infected. This Centre provides blood for transfusions in Abidjan and the surrounding area, which from 1992 to 1999 collected 263,398 blood units. In this period, 5574 subjects were detected HIV-positive. Among those, 1766 (32%) HIV infected blood donors came back to be tested for confirmation of HIV diagnosis. Since then, only 9% of the 5574 donors have been seen at least twice a year for medical and psychological follow-up. Women were more compliant than men in the FonSIDA Clinic: they constituted 62% of the 409 patients who were followed-up (p < 0.001). 53% of men had sex with prostitutes the year before HIV diagnosis. 67% of women stated voluntary abortion at least once. In the same period the systematic use of condoms was reported by only 7% of women and 5% of men. 22% of women and 28% of men reported having two or more sexual partners in the year before HIV diagnosis. The main aim of every blood center is to improve blood safety, particularly in developing countries. The appropriate counseling towards blood donors and especially those detected HIV positive can contribute to reduce new HIV infections in high HIV prevalence cities. Rate of compliance of HIV-infected patients to follow-up has risen to 11% in 1992-1994 to 60% in 1997-1999 and will contribute to reach this aim.

  19. A comparison of the failure times of pulse oximeters during blood pressure cuff-induced hypoperfusion in volunteers.

    Science.gov (United States)

    Kawagishi, Toshiya; Kanaya, Noriaki; Nakayama, Masayasu; Kurosawa, Saori; Namiki, Akiyoshi

    2004-09-01

    Important information may not be obtained if the pulse oximetry signal is lost during inflation of a cuff for blood pressure measurement, particularly in patients with hemodynamic instability. In the present study, we compared the failure times of pulse oximeters during cuff-induced hypoperfusion in volunteers. A pulse oximeter sensor was attached to the index finger, and a blood pressure cuff was attached to the same arm of each volunteer. MasimoSET Radical (Masimo), Nellcor N-395 (N-395), Nellcor N-20PA, and Nellcor D-25 were tested. To evaluate the failure time of each pulse oximeter, time to peak of cuff pressure, time to loss of signal, time to recovery of signal, and failure interval were measured. All measurements were performed three times for each pulse oximeter and were averaged. There were no differences in hemodynamic measurements among the groups. Time to loss of signal was longer in Masimo than the other pulse oximeters. Masimo and N-395 showed significantly shorter times to recovery of signal than those of the other two pulse oximeters. Failure interval was in the order of Masimo Masimo did not lose a signal as rapidly as the other oximeters studied. Masimo was similar in performance to the N-395 at providing useful data sooner than conventional technology after a loss of the signal. These observations suggest that data will be more available with fewer false-positive alarms when using the Masimo oximeter followed by the N-395 when compared with conventional oximeters.

  20. Prevalence of human T-cell lymphotropic virus types 1 and 2 in blood donors of the Caruaru Blood Center (Hemope

    Directory of Open Access Journals (Sweden)

    Waleska Mayara Gomes de Lima

    2013-01-01

    Full Text Available BACKGROUND: There is difficulty in gathering data on the prevalence of human T-cell lymphotropic virus in blood donors as confirmatory testing is not mandatory in Brazil. This suggests there may be an underreporting of the prevalence. OBJECTIVE: To estimate the prevalence of human T-cell lymphotropic virus types 1 and 2 in donors of a blood bank in Caruaru, Brazil. METHODS: This was an observational, epidemiological, descriptive, longitudinal and retrospective study with information about the serology of donors of the Caruaru Blood Center, Fundação de Hematologia e Hemoterapia de Pernambuco (Hemope from May 2006 to December 2010. The data were analyzed using the Excel 2010 computer program (Microsoft Office(r. RESULTS: Of 61,881 donors, 60 (0.096% individuals were identified as potential carriers of human T-cell lymphotropic virus types 1 and 2. Of these, 28 (0.045% were positive and 32 (0.051% had inconclusive results in the serological screening. Forty-five (0.072% were retested; 17 were positive (0.027% and 3 inconclusive (0.005%. After confirmatory tests, 8 were positive (0.013%. Six (75% of the confirmed cases were women. CONCLUSION: Epidemiological surveys like this are very important in order to create campaigns to attract donors and reduce the costs of laboratory tests.

  1. Prevalence of human T-cell lymphotropic virus types 1 and 2 in blood donors of the Caruaru Blood Center (Hemope).

    Science.gov (United States)

    de Lima, Waleska Mayara Gomes; Esteves, Fabrício Andrade Martins; Torres, Maria do Carmo Morais Rodrigues; Pires, Edna Suely Feitosa

    2013-01-01

    There is difficulty in gathering data on the prevalence of human T-cell lymphotropic virus in blood donors as confirmatory testing is not mandatory in Brazil. This suggests there may be an underreporting of the prevalence. To estimate the prevalence of human T-cell lymphotropic virus types 1 and 2 in donors of a blood bank in Caruaru, Brazil. This was an observational, epidemiological, descriptive, longitudinal and retrospective study with information about the serology of donors of the Caruaru Blood Center, Fundação de Hematologia e Hemoterapia de Pernambuco (Hemope) from May 2006 to December 2010. The data were analyzed using the Excel 2010 computer program (Microsoft Office(®)). Of 61,881 donors, 60 (0.096%) individuals were identified as potential carriers of human T-cell lymphotropic virus types 1 and 2. Of these, 28 (0.045%) were positive and 32 (0.051%) had inconclusive results in the serological screening. Forty-five (0.072%) were retested; 17 were positive (0.027%) and 3 inconclusive (0.005%). After confirmatory tests, 8 were positive (0.013%). Six (75%) of the confirmed cases were women. Epidemiological surveys like this are very important in order to create campaigns to attract donors and reduce the costs of laboratory tests.

  2. Time trend of the prevalence of hepatitis E antibodies among farmers and blood donors: a potential zoonosis in Denmark

    DEFF Research Database (Denmark)

    Christensen, Peer B; Engle, Ronald E; Hjort, Charlotte

    2008-01-01

    BACKGROUND: Antibody to hepatitis E virus (anti-HEV) is prevalent in Western countries, where clinical hepatitis E is rarely reported. The aim of this study was to determine the prevalence of anti-HEV among Danish blood donors and Danish farmers. In addition, we compared the prevalence among 2 se...

  3.  Glucose-6-Phosphate Dehydrogenase Deficiency among Male Blood Donors inSana’a City, Yemen

    Directory of Open Access Journals (Sweden)

    Molham AL-Habori

    2012-01-01

    Full Text Available  Objectives: To determine the prevalence of Glucose-6-phosphatedehydrogenase (G-6-PD deficiency among Yemeni people fromdifferent regions of the country living in the capital city, Sana’a,giving an indication of its overall prevalence in Yemen.Methods: A cross-sectional study was conducted among Yemenimale blood donors attending the Department of Blood Bank atthe National Centre of the Public Health Laboratories in thecapital city, Sana’a, Yemen. Fluorescent spot method was used forscreening, spectrophotometeric estimation of G-6-PD activityand separation by electrophoresis was done to determine the G-6-PD phenotype.Results: Of the total 508 male blood donors recruited into thestudy, 36 were G-6-PD deficient, giving a likely G-6-PD deficiencyprevalence of 7.1�20None of these deficient donors had history ofanemia or jaundice. Thirty-five of these deficient cases (97.2�howed severe G-6-PD deficiency class II (<10�0of normalactivity, and their phenotyping presumptively revealed a G-6-PDMediterraneanvariant.Conclusion: The results showed a significant presence of G-6-PD deficiency with predominance of a severe G-6-PD deficiencytype in these blood donors in Sana’a City, which could representan important health problem through occurrence of hemolyticanemia under oxidative stress. A larger sample size is needed todetermine the overall prevalence of G-6-PD deficiency, and shouldbe extended to include DNA analysis to identify its variants in Yemen.

  4. Seroprevalence of antibodies to hepatitis E virus in the normal blood donor population and two aboriginal communities in Malaysia.

    Science.gov (United States)

    Seow, H F; Mahomed, N M; Mak, J W; Riddell, M A; Li, F; Anderson, D A

    1999-10-01

    The prevalence of antibodies to hepatitis E virus (HEV) has been examined in many countries, but such studies have generally been limited to majority populations such as those represented in healthy blood donors or cross sections of urban populations. Due to its major route of enteric transmission, large differences in HEV prevalence might be expected between populations in the same country but with different living conditions. Using an ELISA based on GST-ORF2.1 antigen, the prevalence of IgG-class antibodies to HEV was examined in three distinct populations in Malaysia: the normal (urban) blood donor population and two aboriginal communities located at Betau, Pahang and Parit Tanjung, Perak. IgG anti-HEV was detected in 45 (44%) of 102 samples from Betau and 15 (50%) of 30 samples from Parit Tanjung, compared to only 2 (2%) of 100 normal blood donors. The distribution of sample ELISA reactivities was also consistent with ongoing sporadic infection in the aboriginal communities, while there was no significant relationship between HEV exposure and age, sex, or malaria infection. The high prevalence of antibodies to HEV in the two aboriginal communities indicates that this group of people are at high risk of exposure to HEV compared to the general blood donors, and the results suggest that studies of HEV seroprevalence within countries must take into account the possibility of widely varying infection rates between populations with marked differences in living conditions.

  5. A nationwide survey of hepatitis E viral infection in French blood donors.

    Science.gov (United States)

    Mansuy, Jean Michel; Gallian, Pierre; Dimeglio, Chloé; Saune, Karine; Arnaud, Catherine; Pelletier, Bertrand; Morel, Pascal; Legrand, Dominique; Tiberghien, Pierre; Izopet, Jacques

    2016-04-01

    Most cases of hepatitis E viral (HEV) infection in developed countries are autochthonous. Nevertheless, the reported seroprevalence of HEV varies greatly depending on the geographical area and the performance of the immunoassay used. We used validated assays to determine the prevalence of anti-HEV immunoglobulin G (IgG) and IgM among 10,569 French blood donors living in mainland France and three overseas areas. Epidemiological information was collected using a specific questionnaire. We found an overall IgG seroprevalence of 22.4% (8%-86.4%) depending on the geographical area (P < 0.001). The presence of anti-HEV IgG was associated with increasing age (P < 0.001) and eating pork meat (P = 0.03), pork liver sausages (P < 0.001), game meat (P < 0.01), offal (P < 0.001), and oysters (P = 0.02). Conversely, drinking bottled water was associated with a lower rate of anti-HEV IgG (P = 0.02). Overall IgM seroprevalence was 1% (0%-4.6%). The frequency of anti-HEV IgM was higher in donors living in a high anti-HEV IgG seroprevalence area (1.9% versus 0.7%, P < 0.001) and in those eating pork liver sausage (1.4% versus 0.7%, P < 0.01), pâté (1% versus 0.4, P = 0.04), and wild boar (1.3% versus 0.7%, P < 0.01). HEV is endemic in France and hyperendemic in some areas; eating habits alone cannot totally explain the exposure to HEV, and contaminated water could contribute to the epidemiology of HEV infection in France. © 2015 by the American Association for the Study of Liver Diseases.

  6. Specific autoantibodies precede the symptoms of rheumatoid arthritis: a study of serial measurements in blood donors.

    Science.gov (United States)

    Nielen, Markus M J; van Schaardenburg, Dirkjan; Reesink, Henk W; van de Stadt, Rob J; van der Horst-Bruinsma, Irene E; de Koning, Margret H M T; Habibuw, Moud R; Vandenbroucke, Jan P; Dijkmans, Ben A C

    2004-02-01

    Autoantibodies have been demonstrated in single serum samples from healthy subjects up to 10 years before they developed rheumatoid arthritis (RA). However, the time course for the development of antibodies before onset of clinical RA is unknown, nor is it known which antibody, or combinations of antibodies, might be most sensitive or specific for predicting future development of the disease. The present study was undertaken to investigate this. Patients with RA who had been blood donors before the onset of disease symptoms were enrolled. Frozen serum samples from each donor were retrieved, together with 2 serum samples from controls matched for age, sex, and date of donation. All samples were tested for IgM rheumatoid factor (IgM-RF) and anti-cyclic citrullinated peptide (anti-CCP) antibodies. Seventy-nine patients with RA (62% female; mean age at onset of symptoms 51 years) were included. A median of 13 samples (range 1-51) per patient were available; the earliest samples had been collected a median of 7.5 years (range 0.1-14.5) before the onset of symptoms. Thirty-nine patients (49%) were positive for IgM-RF and/or anti-CCP on at least one occasion before the development of RA symptoms, a median of 4.5 years (range 0.1-13.8) before symptom onset. Of the 2,138 control samples, 1.1% were positive for IgM-RF, and 0.6% were positive for anti-CCP. Approximately half of patients with RA have specific serologic abnormalities several years before the onset of symptoms. A finding of an elevated serum level of IgM-RF or anti-CCP in a healthy individual implies a high risk for the development of RA. We conclude that IgM-RF and anti-CCP testing with appropriately high specificity may assist in the early detection of RA in high-risk populations.

  7. How to encourage non-donors to be more willing to donate blood? Testing of binding communication based interventions.

    Science.gov (United States)

    Fonte, D; Blondé, J; Girandola, F

    2017-06-01

    Our study aims to test the effectiveness of binding communication based interventions (vs classical persuasive communication based ones) inciting non-donors to act in favour of blood donation. The implementation of effective communication interventions represents a major public health issue. Nevertheless, persuasive media campaigns appear to have little effect on behaviours. Even though non-donors hold a positive attitude towards blood donation, they are not inclined to donate. As an alternative to producing behavioural changes, many recent studies have shown the superiority of binding communication over persuasive communication. All participants, non-donors, were randomly assigned to one of four experimental conditions of a 2 (type of communication: persuasive vs binding) × 2 (source credibility: low vs high) factorial design. Then, they were asked to report their intention to donate blood, and their intention to distribute leaflets regarding blood donation. Binding communication is a more effective strategy for increasing intention towards blood donation compared with persuasive communication, especially when combined with high credibility source. Accordingly this study calls for more consideration of knowledge of social psychology to design effective communication interventions and increase the number of donations. © 2016 British Blood Transfusion Society.

  8. Seroprevalence of malaria in blood donors and multi-transfused patients in Northern India: Relevance to prevention of transfusion-transmissible malaria

    Directory of Open Access Journals (Sweden)

    Anju Dubey

    2012-01-01

    Full Text Available Background: Transfusion-transmissible malaria (TTM is a major concern in malaria endemic countries. A study was therefore conducted to know sero-prevalence of malaria in blood donors and the risk of TTM to multi-transfused patients at our hospital. Materials and Methods: Study subjects were: eligible blood donors (n = 1000, donors deferred due to history of fever in the last 3 months (n = 100, and multi-transfused patients (n = 200. Screening for malaria was done by slide microscopy, immunochromatographic rapid diagnostic test (RDT for malaria antigen, and anti-malaria antibody by enzyme linked immunosorbent assay. Results: Malaria antibody prevalence in eligible donors and donors with history of fever, thalassemia patients, and in other multi-transfused patients was 16.9%, 22%, 6%, and 15%, respectively. None of the donors were positive for malaria on microscopic examination. None of the blood donors except one donor with history of fever, tested positive with RDT. Conclusion: Malaria antibody prevalence in blood donors at our center is high. As blood units donated by such donors have high-risk potential, special processing may be undertaken to reduce the risk of TTM.

  9. Genetic Diversity of Recently Acquired and Prevalent HIV, Hepatitis B Virus, and Hepatitis C Virus Infections in US Blood Donors

    Science.gov (United States)

    Slikas, Elizabeth; Stramer, Susan L.; Kamel, Hany; Kessler, Debra; Krysztof, David; Tobler, Leslie H.; Carrick, Danielle M.; Steele, Whitney; Todd, Deborah; Wright, David J.; Kleinman, Steven H.; Busch, Michael P.

    2012-01-01

    (See the editorial commentary by Katz, on pages 867–9 and see the article by Stramer et al, on pages 886–94.) Background. Genetic variations of human immunodeficiency virus (HIV), hepatitis C virus (HCV), and hepatitis B virus (HBV) can affect diagnostic assays and therapeutic interventions. Recent changes in prevalence of subtypes/genotypes and drug/immune-escape variants were characterized by comparing recently infected vs more remotely infected blood donors. Methods. Infected donors were identified among approximately 34 million US blood donations, 2006–2009; incident infections were defined as having no or low antiviral antibody titers. Viral genomes were partially sequenced. Results. Of 321 HIV strains (50% incident), 2.5% were non-B HIV subtypes. Protease and reverse transcriptase (RT) inhibitor resistance mutations were found in 2% and 11% of infected donors, respectively. Subtypes in 278 HCV strains (31% incident) yielded 1a>1b>3a>2b>2a>4a>6d, 6e: higher frequencies of 3a in incident cases vs higher frequencies of 1b in prevalent cases were found (P = .04). Twenty subgenotypes among 193 HBV strains (26% incident) yielded higher frequencies of A2 in incident cases and higher frequencies of A1, B2, and B4 in prevalent cases (P = .007). No HBV drug resistance mutations were detected. Six percent of incident vs 26% of prevalent HBV contained antibody neutralization escape mutations (P = .01). Conclusions. Viral genetic variant distribution in blood donors was similar to that seen in high-risk US populations. Blood-borne viruses detected through large-scale routine screening of blood donors can complement molecular surveillance studies of highly exposed populations. PMID:22293432

  10. HIV, HBV, HCV and T. pallidum infections among blood donors and Transfusion-related complications among recipients at the Laquintinie hospital in Douala, Cameroon

    OpenAIRE

    Eboumbou Moukoko, Carole Else; Ngo Sack, Françoise; Essangui Same, Estelle Géraldine; Mbangue, Madeleine; Lehman, Léopold Gustave

    2014-01-01

    Background Transfusion-transmissible infections (TTIs) pose a major health risk in Cameroon given the high prevalence of such pathogens and increased demands for blood donations in the local communities. This study aims at establishing the prevalence of commonly encountered TTIs among blood donors and transfusion-related complications among recipients in an urban center of Cameroon. Methods A total of 477 blood donors and 83 blood recipients were recruited by consecutive sampling at the Laqui...

  11. Results of the Cord Blood Transplantation Study (COBLT) : clinical outcomes of unrelated donor umbilical cord blood transplantation in pediatric patients with hematologic malignancies

    NARCIS (Netherlands)

    Kurtzberg, Joanne; Prasad, Vinod K.; Carter, Shelly L.; Wagner, John E.; Baxter-Lowe, Lee Ann; Wall, Donna; Kapoor, Neena; Guinan, Eva C.; Feig, Stephen A.; Wagner, Elizabeth L.; Kernan, Nancy A.

    2008-01-01

    Outcomes of unrelated donor cord blood transplantation in 191 hematologic malignancy children (median age, 7.7 years; median weight, 25.9 kg) enrolled between 1999 and 2003 were studied (median follow-up, 27.4 months) in a prospective phase 2 multicenter trial. Human leukocyte antigen (HLA) matching

  12. Results of the Cord Blood Transplantation Study (COBLT) : clinical outcomes of unrelated donor umbilical cord blood transplantation in pediatric patients with hematologic malignancies

    NARCIS (Netherlands)

    Kurtzberg, Joanne; Prasad, Vinod K.; Carter, Shelly L.; Wagner, John E.; Baxter-Lowe, Lee Ann; Wall, Donna; Kapoor, Neena; Guinan, Eva C.; Feig, Stephen A.; Wagner, Elizabeth L.; Kernan, Nancy A.

    2008-01-01

    Outcomes of unrelated donor cord blood transplantation in 191 hematologic malignancy children (median age, 7.7 years; median weight, 25.9 kg) enrolled between 1999 and 2003 were studied (median follow-up, 27.4 months) in a prospective phase 2 multicenter trial. Human leukocyte antigen (HLA) matching

  13. Blood transfusion and iatrogenic risks in Mexico city: anti-Trypanosoma cruzi seroprevalence in 43,048 blood donors, evaluation of parasitemia, and electrocardiogram findings in seropositive

    Directory of Open Access Journals (Sweden)

    Nidia Hernández-Becerril

    2005-04-01

    Full Text Available Iatrogenous transmission of Trypanosoma cruziby blood transfusion was suggested as a potential risk by Pellegrino (1949. Seropositive blood donors in Mexico were first reported in 1978, however, limited information is available due to small sampling, the use of heterogeneous serologic assays, and geographically limited studies. A wide survey carried out in 18 out of the 32 states of Mexico, showed a national mean of 1.6% seropositive among 64,969 donors, ranging from 0.2 to 2.8%. In the present study, we have screened 43,048 voluntary blood donors in a period of five years at the Instituto Nacional de Cardiología I. Chávez, a concentration hospital located in Mexico city which serves mainly the metropolitan area and accepts from all over the country. Standardized ELISA and IIF were used to identify seropositive individuals in addition to hemoculture, PCR and standard 12 lead ECG tests that were applied to a group of seropositive patients (29/161. The result showed a seropositivity of 0.37% (161/43,048. From the group of seropositive individuals 40% (12/29 were potential carriers of T. cruzi at the donation time and 5/29 had subclinical ECG abnormalities. Parasitological tests performed in 70 erythrocyte and platelet fractions from seropositive units (70/161 showed negative results. Our findings strongly support T. cruzi screening in the transfusion medicine practice and identify subclinical heart disease among seropositive blood donors.

  14. Failure of self-disclosure of deferrable risk behaviors associated with transfusion-transmissible infections in blood donors.

    Science.gov (United States)

    Wong, Horas Tze Hoo; Lee, Shui Shan; Lee, Cheuk-Kwong; Chan, Denise Pui Chung

    2015-09-01

    To date, most studies on deferral of blood donors have focused on men who have sex with men (MSM) and/or injecting drug users. Few have examined deferrable risk behaviors relating to transfusion-transmissible infections (TTIs) in general. This study aimed to examine the prevalence of, and factors associated with, nondisclosure of TTI-related risk behaviors in donors. Chinese-speaking donors who had just given blood in Hong Kong were invited to self-complete an anonymous questionnaire. Practices of one or more of seven deferrable risk behaviors associated with TTI were inquired. Factors associated with noncompliance with self-disclosure were evaluated by logistic regression. Over a 4-week study period in 2012, a total of 1143 donors were recruited. Overall, 0.2% gave a history of drug injection, 1.7% had had sex with sex worker(s), and 0.3% had had sex with a human immunodeficiency virus-infected partner, while none had been paid for sex. Some 1.5% of male donors reported having same-sex behaviors. Factors associated with noncompliance were male gender (odds ratio [OR] 31.1; 95% confidence interval [CI], 3.7-263.6), having multiple sex partners (OR, 89.7; 95% CI, 28.7-279.9), and previous history of temporary deferral (OR, 11.4; 95% CI, 2.5-53.3). If suspected noncompliance was included, the overall prevalence of nondisclosure of deferrable behaviors could be high at 6.5%. Albeit uncommon, some donors fail to provide accurate answers to predonation screening questions and are not deferred appropriately. There is room for improvement to make deferral policy acceptable and understandable, so as to minimize the risk of TTI. Efforts are also needed to tackle the paucity of data on noncompliance of non-MSM donors. © 2015 The Authors Transfusion published by Wiley Periodicals, Inc. on behalf of AABB.

  15. Electrocardiographic and blood pressure effects of energy drinks and Panax ginseng in healthy volunteers: A randomized clinical trial.

    Science.gov (United States)

    Shah, Sachin A; Occiano, Andrew; Nguyen, Tinh An; Chan, Amanda; Sky, Joseph C; Bhattacharyya, Mouchumi; O'Dell, Kate M; Shek, Allen; Nguyen, Nancy N

    2016-09-01

    Energy drink usage has been linked to emergency room visits and deaths. The objective of the study is to assess the electrocardiographic and blood pressure effects of energy drinks, Panax ginseng and placebo in healthy individuals. This was a randomized, double blinded, placebo controlled, crossover study. Young healthy volunteers with no comorbid conditions consumed 32oz of an energy drink, control drink with 800mg of Panax ginseng or matching placebo-control drink over 45min. Primary endpoints were QTc interval and systolic blood pressure. Secondary endpoints included QT interval, PR interval, QRS duration, heart rate, and diastolic blood pressure. All endpoints were assessed at baseline, 1, 2, 3.5, and 5.5h. A significant increase in QTc interval 2h post energy drink consumption was evident when compared to placebo (3.37±10.7ms and -3.19±11.8ms respectively; p=0.030). Similarly, systolic blood pressure 2h post energy drink consumption increased when compared to placebo (2.00±6.37mmHg and -2.67±5.83mmHg respectively; p=0.014). The PR interval significantly reduced over a 2h period post energy drink use in a clinically non-meaningful manner. Heart rate at 2h was not significantly higher in the energy drink group when compared to others. The QT interval, QRS interval and diastolic blood pressure were not impacted at any time point. Certain energy drinks consumed at a high volume significantly increase the QTc interval and systolic blood pressure by over 6ms and 4mmHg respectively. Panax ginseng does not have a significant impact on ECG or blood pressure parameters. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  16. Na2S, a fast-releasing H2S donor, given as suppository lowers blood pressure in rats.

    Science.gov (United States)

    Tomasova, Lenka; Drapala, Adrian; Jurkowska, Halina; Wróbel, Maria; Ufnal, Marcin

    2017-04-19

    Hydrogen sulfide (H2S) is involved in blood pressure control. The available slow-releasing H2S-donors are poorly soluble in water and their ability to release H2S in biologically relevant amounts under physiological conditions is questionable. Therefore, new slow-releasing donors or new experimental approaches to fast-releasing H2S donors are needed. Hemodynamics and ECG were recorded in male, anesthetized Wistar Kyoto rats (WKY) and in Spontaneously hypertensive rats (SHR) at baseline and after: 1) intravenous (iv) infusion of vehicle or Na2S; 2) administration of vehicle suppositories or Na2S suppositories. Intravenously administered vehicle and vehicle suppositories did not affect mean arterial blood pressure (MABP) and heart rate (HR). Na2S administered iv caused a significant, but transient (2-5min) decrease in MABP. Na2S suppositories produced a dose-dependent hypotensive response that lasted ∼45min in WKY and ∼75-80min in SHR. It was accompanied by a decrease in HR in WKY, and an increase in HR in SHR. Na2S suppositories did not produce a significant change in corrected QT, an indicator of cardiotoxicity. Na2S suppositories increased blood level of thiosulfates, products of H2S oxidation. Na2S administered in suppositories exerts a prolonged hypotensive effect in rats, with no apparent cardiotoxic effect. SHR and WKY differ in hemodynamic response to the H2S donor. Suppository formulation of fast-releasing H2S donors may be useful in research, if a reference slow-releasing H2S donor is not available. Copyright © 2017 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.

  17. Near-infrared spectroscopy extended with indocyanine green dye dilution for cerebral blood flow measurement: Median values in healthy volunteers

    Science.gov (United States)

    Mudra, R.; Muroi, C.; Niederer, P.; Keller, E.

    2008-09-01

    The cerebral blood flow (CBF) is an important vital parameter in neurointensive care. Currently, there is no non-invasive method for its measurement that can easily be applied at the bedside. A new tool to determine CBF is based on near-infrared spectroscopy (NIRS) applied together with indocyanine green (ICG) dye dilution. From a bilateral measurement on selected regions on the head of infrared (IR) absorption at various wavelengths during the dilution maneuver, the vascular perfusion characteristics of the two brain hemispheres can be determined in terms of mean transit time (mtt) of ICG, cerebral blood volume (CBV) and CBF. So far, on nine healthy volunteers, NIRS ICG dye dilution bihemispheric measurements were performed, which yielded to mtt given as median (range) of 9.3 s (5.1-16.3 s), CBV of 3.5 ml/100 g (1.7-4.1 ml/100 g), and CBF of 18.2 ml/(100 g×min) [11.1-48.6 ml/(100 g×min)]. Additionally, the blood flow index (BFI) was calculated with BFI= 13.8 mg/(100 g×s) [6.6-15.2 mg/(100 g×s)]. The Spearman rank correlation coefficient between CBF and BFI was RS = 0.76. However, as the Bland & Altman plot between CBFNIRS and the CBFBFI documents, the limits of agreement are rather wide (21.9±6.7). Under physiological conditions in healthy volunteers, no differences could be detected between the hemispheres.

  18. Non-detection of human herpesvirus 8 (HHV-8 DNA in HHV-8-seropositive blood donors from three Brazilian regions.

    Directory of Open Access Journals (Sweden)

    José Eduardo Levi

    Full Text Available Human herpesvirus 8 (HHV-8, also known as Kaposi's sarcoma-associated herpesvirus (KSHV, is the etiologic agent of all forms of Kaposi's sarcoma, primary effusion lymphoma and the plasmablastic cell variant of multicentric Castleman disease. In endemic areas of sub-Saharan Africa, blood transfusions have been associated with a substantial risk of HHV-8 transmission. By contrast, several studies among healthy blood donors from North America have failed to detect HHV-8 DNA in samples of seropositive individuals. In this study, using a real-time PCR assay, we investigated the presence of HHV-8 DNA in whole-blood samples of 803 HHV-8 blood donors from three Brazilian states (São Paulo, Amazon, Bahia who tested positive for HHV-8 antibodies, in a previous multicenter study. HHV-8 DNA was not detected in any sample. Our findings do not support the introduction of routine HHV-8 screening among healthy blood donors in Brazil. (WC = 140.

  19. Seroprevalence of Strongyloides stercoralis infection among HTLV-I infected blood donors in Barcelona, Spain: A cross-sectional study.

    Science.gov (United States)

    Salvador, Fernando; Sulleiro, Elena; Piron, Maria; Sánchez-Montalvá, Adrián; Sauleda, Silvia; Molina, Israel

    2017-09-20

    Strongyloides stercoralis infection in patients with HTLV-I infection may lead to severe clinical manifestations. The aim of the present study is to determine the seroprevalence of S. stercoralis infection among blood donors who tested positive for HTLV-I infection. A cross-sectional study was performed at the Vall d'Hebron University Hospital (Barcelona, Spain) in 2016. Serum samples from HTLV-I positive patients diagnosed from 2008 to 2015 were retrieved from the Blood Bank, and S. stercoralis serology was performed. Thirty six serum samples from HTLV-I positive patients were retrieved from the Blood Bank. The blood samples came from 36 blood donors, and most of them were born in Latin America (75%), being Peru the most frequent country (11 participants). S. stercoralis serology was positive in one patient, corresponding to a prevalence of 2.8% (3.4% if we exclude donors coming from European countries, where the risk of S. stercoralis infection is highly unlikely). Copyright © 2017 Elsevier B.V. All rights reserved.

  20. Oral or parenteral iron supplementation to reduce deferral, iron deficiency and/or anaemia in blood donors.

    Science.gov (United States)

    Smith, Graham A; Fisher, Sheila A; Doree, Carolyn; Di Angelantonio, Emanuele; Roberts, David J

    2014-07-03

    Iron deficiency is a significant cause of deferral in people wishing to donate blood. If iron removed from the body through blood donation is not replaced, then donors may become iron deficient. All donors are screened at each visit for low haemoglobin (Hb) levels. However, some deferred blood donors do not return to donate. Deferred first-time donors are even less likely to return. Interventions that reduce the risk of provoking iron deficiency and anaemia in blood donors will therefore increase the number of blood donations. Currently, iron supplementation for blood donors is not a standard of care in many blood services. A systematic review is required to answer specific questions regarding the efficacy and safety of iron supplementation in blood donors. To assess the efficacy and safety of iron supplementation to reduce deferral, iron deficiency and/or anaemia in blood donors. We ran the search on 18 November 2013. We searched Cochrane Injuries Group Specialised Register, CENTRAL, PubMed, MEDLINE (OvidSP), EMBASE (OvidSP), CINAHL (EBSCO Host) and six other databases. We also searched clinical trials registers and screened guidelines reference lists. Randomised controlled trials (RCTs) comparing iron supplementation versus placebo or control, oral versus parenteral iron supplementation, iron supplementation versus iron-rich food supplements, and different doses, treatment durations and preparations of iron supplementation in healthy blood donors. Autologous blood donors were excluded. We combined data using random-effects meta-analyses. We evaluated heterogeneity using the I(2) statistic; we explored considerable heterogeneity (I(2) > 75%) in subgroup analyses. We carried out sensitivity analyses to assess the impact of trial quality on the results. Thirty RCTs (4704 participants) met the eligibility criteria, including 19 comparisons of iron supplementation and placebo or control; one comparison of oral and parenteral iron supplementation; four comparisons of

  1. Seropositivity rates of HBsAg, anti-HCV, anti-HIV and VDRL in blood donors in Eastern Turkey.

    Science.gov (United States)

    Dilek, İmdat; Demir, Cengiz; Bay, Ali; Akdeniz, Hayrettin; Öner, Ahmet Faik

    2007-03-05

    Infections caused by hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency viruses (HIV) remain the leading most important health problems worldwide. Screening tests such as HBsAg, anti-HCV, anti-HIV and VDRL are mandatory tests to look at before transfusion of blood or blood components. In this study, donors who applied to our Blood Center in a nine-year period were retrospectively evaluated with respect to HBsAg, anti-HCV, anti-HIV and syphilis seroprevalence. HBsAg, anti-HCV and anti-HIV were examined by microparticle ELISA system, and syphilis antibodies were screened by a syphilis test device. Of the total 39,002 individuals, 16,601 (42%) were females and 22,401 (58%) were males. HBsAg positivity was found in 2.55%, anti-HCV in 0.17%, anti-HIV in 0.036%, and VDRL in 0.057% of overall donors. As a result, HBsAg, anti-HCV, anti-HIV and VDRL seropositivity rates in donors living in our region were found lower than those in many regions of Turkey. Nevertheless, because there is no screening method to reduce the risk resulting from transfusion to zero, it appears that it is essential to adopt strict criteria in the selection of donors and to avoid unnecessary transfusion.

  2. Giving blood donors something to drink before donation can prevent fainting symptoms: is there a physiological or psychological reason?

    Science.gov (United States)

    Vavic, Nataša; Pagliariccio, Antonella; Bulajic, Milica; Dinić, Radovan; Marinozzi, Maria

    2014-08-01

    The vasovagal reaction has been widely studied but its anatomic and physiological nature remains uncertain. The mechanisms underlying vasovagal reaction related to blood donation are not completely understood either. Does its occurrence depend on the blood donors' physical characteristics and health variables or psychological factors? On the basis that a psychological approach considerably prevents donor reactions, the effect of fruit juice ingestion was studied in a group of 1849 first-time high-school students as a simple strategy to avoid systemic reactions at blood donation. The reasons for the psychological effect of this hydration protocol are stressed also in light of previous physiological studies on the hemodynamic effects of water or carbohydrate drinks.

  3. Human T-Lymphotropic Virus Type I (HTLV-1) Infection among Iranian Blood Donors: First Case-Control Study on the Risk Factors.

    Science.gov (United States)

    Hedayati-Moghaddam, Mohammad Reza; Tehranian, Farahnaz; Bayati, Maryam

    2015-11-04

    Human T-cell lymphotropic virus type 1 (HTLV-1) infection is an endemic condition in Northeast Iran and, as such, identification of risk factors associated with the infection in this region seems to be a necessity. All the possible risk factors for HTLV-1 seropositivity among first-time blood donors were evaluated in Mashhad, Iran, during the period of 2011-2012. Blood donation volunteers were interviewed for demographic data, medical history, and behavioral characteristics and the frequencies of risk factors were compared between HTLV-1 positive (case) and HTLV-1 negative (control) donors. The data was analyzed using Chi square and t-tests. Logistic regression analysis was performed to identify independent risk factors for the infection. Assessments were carried out on 246 cases aged 17-60 and 776 controls aged 17-59, who were matched based on their ages, gender, and date and center of donation. Logistic analysis showed low income (OR = 1.53, p = 0.035), low educational level (OR = 1.64, p = 0.049), being born in the cities of either Mashhad (OR = 2.47, p = 0.001) or Neyshabour (OR = 4.30, p risk factors for HTLV-1 infection, such as prolonged breastfeeding and sexual promiscuity. Pre-donation screening of possible risk factors for transfusion-transmissible infections should also be considered as an important issue, however, a revision of the screening criteria such as a history of transfusion for more than one year prior to donation is strongly recommended.

  4. [The influence of general infrared sauna on the antioxidant systems in the blood of volunteers].

    Science.gov (United States)

    Khodarev, V N; Zhemchuzhnova, N L; Olempieva, E V; Kuz'menko, N V

    2013-01-01

    This comprehensive clinical and laboratory study was designed to elucidate the antioxidant status of the volunteers before and after a course of general infrared irradiation. It was shown that the effect of high temperatures promotes the development of oxidative stress that is followed by the formation of adaptive reactions in the form of activation of antioxidant protection, enhancement of non-specific responses of the cells, increase of stability and restoration of structural homeostasis of erythrocyte membranes. The molecular mechanism of endothelium-independent vasodilation develops by the end of the treatment period; it may serve as the compensatory-adaptive reaction needed to maintain the adequate tone of the vascular wall and thereby to support the functioning of mechanisms supporting physical thermoregulation. The results of the study give evidence of the stimulatory influence of the described method on the adaptive and protective potential of the organism. It is concluded that the proposed scheme of physiotherapeutic treatment may be used for prophylactic purposes.

  5. The prevalence of hepatitis B virus E antigen among Ghanaian blood donors

    Science.gov (United States)

    Rufai, Tanko; Mutocheluh, Mohamed; Kwarteng, Kwaku; Dogbe, Elliot

    2014-01-01

    Hepatitis B viral infection is an important clinical problem due to its worldwide distribution and potential of adverse sequelae, including hepatocellular carcinoma (HCC). We studied the prevalence of hepatitis B virus ‘e’ antigen (HBeAg) among individuals determined to be hepatitis B virus (HBV) surface antigen-positive and analyzed the gender/age category associated with more active HBV infection and whether alteration in the levels of alanine aminotransferase could be associated with HBeAg positivity. A total of 150 prospective blood donors who tested positive for hepatitis B surface antigen (HBsAg) at the blood transfusion center of the Komfo Anokye Teaching Hosptital (KATH), Kumasi were randomly selected for the study. The serum samples were further tested for HBsAg and HBeAg using a lateral flow immunochromatographic assay. Twenty (20) individuals were found to be HBeAg-positive giving an overall prevalence of 13.3%, of which 18 (15.5%) were males and 2 (5.9%) were females. Our results also revealed that the prevalence of HBeAg was higher in patients between the age group of 10-20 years and appeared to decrease with increase in age. There was no statistical difference between the HBeAg positive and negative individuals with respect to alanine aminotransferase (ALT) levels. We show for the first time that approximately 1/10 of HBV-infected individuals are HBeAg positive in the Ashanti Region of Ghana, suggestive of active viral replication and liver-cell infectivity thereby contributing to an increased HBV-transmission pool within the Ghanaian population. PMID:25018803

  6. Plasma protein fractions in healthy blood donors quantitated by an automated multicapillary electrophoresis system.

    Science.gov (United States)

    Larsson, Anders; Hansson, Lars-Olof

    2006-09-01

    During the last decade, capillary electrophoresis (CE) has emerged as an important alternative to traditional analysis of serum and plasma proteins by agarose or celluloseacetate electrophoresis. CE analysis of plasma proteins can now be fully automated and also includes bar-code identification of samples, preseparation steps, and direct post-separation quantitation of individual peaks, which permits short assay times and high throughput. For laboratory work, it is important to have reference values from healthy individuals. Therefore, plasma samples from 156 healthy blood donors (79 females and 77 males) have been analyzed with the Capillarys instrument and the new high resolution buffer, which yields higher resolution than the beta1-beta2+ buffer. Albumin concentrations in samples are measured using nephelometry in order to assign protein concentrations to each peak. The 2.5 and 97.5 percentiles for both the percentages of different peaks and the protein concentrations in the peaks are calculated according to the recommendations of the International Federation of Clinical Chemistry on the statistical treatment of reference values. The Capillarys instrument is a reliable system for plasma protein analysis, combining advantages of full automation with high analytical performances and throughput.

  7. Avaliação laboratorial da deficiência de ferro em doadoras de sangue Laboratory analysis of iron deficiency in female blood donors

    Directory of Open Access Journals (Sweden)

    Rodolfo D. Cançado

    2007-06-01

    Full Text Available O objetivo deste estudo foi avaliar a freqüência da deficiência de ferro em doadoras de sangue do Hemocentro da Santa Casa de São Paulo segundo o tipo de doador, o número total de doações anteriores e a freqüência de doações realizadas nos últimos 12 meses. No período de 05 a 20 de outubro de 2004 foram estudadas 100 doadoras de sangue utilizando-se a determinação da ferritina sérica e dos índices eritrocitários. A freqüência de doadoras de sangue que apresentavam deficiência de ferro foi de 16,0 %. Para as doadoras de primeira vez, 10,5% delas já apresentavam deficiência de ferro à primeira doação de sangue e, para aquelas que não eram de primeira vez, essa freqüência foi de 17,7% (pThe aim of this study was to evaluate the frequency of iron deficiency in blood donors at the Santa Casa Blood Bank and to establish the frequency of iron deficient blood donors according to first-time and multiple donors, the total number of lifetime donations and the frequency of donations per year. Between 5th and 20th October 2004, one hundred blood donors were studied using the biochemical measurement of serum ferritin and red blood cell indices. The frequency of iron deficiency in blood donors was 16.0%. The frequency of iron deficiency was higher in non-first-time than in first-time female blood donors (10.5% versus 17.7%; p<0.05. The frequency of iron deficiency was higher in the multiple blood donors and this difference was statistically significative in female blood donors after more than four donations (p<0.05 and among female blood donors with two or more donations per year (p<0.05. We conclude that blood donation is a very important factor of iron deficiency in female blood donors, particularly in multiple donors. The high frequency of blood donors with iron deficiency found in this study suggests the necessity for more accurate laboratory screening, as the hemoglobin or hematocrit measurements alone are insufficient to

  8. Is current serologic RhD typing of blood donors sufficient for avoiding immunization of recipients? (CME)

    DEFF Research Database (Denmark)

    Krog, Grethe Risum; Clausen, Frederik Banch; Berkowicz, Adela

    2011-01-01

    BACKGROUND: Avoiding immunization with clinically important antibodies is a primary objective in transfusion medicine. Therefore, it is central to identify the extent of D antigens that escape routine RhD typing of blood donors and to improve methodology if necessary. STUDY DESIGN AND METHODS: We......-specific primers. Lookback was performed on all recipients of RBCs from RHD+ donors. RESULTS: We found 13 RHD+ samples (0.26%). No variants or chimeras were found. Characterization of DNA revealed a novel DEL type (IVS2-2 A>G). In the lookback of the 136 transfusions with subsequent antibody follow-up, of which 13...... were from DEL donors, one recipient developed anti-D. However, in this case, a competing and more likely cause of immunization was the concurrent transfusion of D+ platelets. Eleven recipients were immunized with 13 antibodies different from anti-D, of which five were anti-K. CONCLUSION: In our...

  9. Investigation on the Distribution of Regular Blood Donors in Urban Wenzhou%温州市区固定献血者分布调查

    Institute of Scientific and Technical Information of China (English)

    谢熙熙; 苏虹; 陈通; 陈李

    2012-01-01

    Objective To establish regular non-remunerated blood donors team through investigation on the urban regular blood donors in Wenzhou. Methods The relationship between donation times and the ages and blood types was analyzed, and the distribution of regular blood donors and blood components donors was contrasted. Results The difference of donation times among different aged regular donors is statistically significant (P 0.05 ). Occupation distribution and educational level distribution between regular blood donors and blood components donors were different. Conclusion Publicity should be strengthened, and identification of the target population of the fixed blood donors with components donors could improve the recruitment efficiency,consolidate and enlarge the regular blood donors population.%目的 通过对温州市区固定献血者人群分布调查,为建立一支固定献血者队伍提供依据.方法 分析献血次数与年龄、血型的关系,对固定献血者人群与其中成分献血者分布情况进行对比分析.结果 不同年龄固定献血者献血次数差异有统计学意义(P<0.05);不同血型固定献血者献血次数差异无有统计学意义(P>0.05).固定献血者与参与成分献血人群在职业与文化程度分布情况有差别.结论 加大宣传,明确招募固定献血者与成分献血者目标人群,可以提高固定献血者招募效率,巩固并扩大固定献血者队伍.

  10. 献血人群红细胞血型意外抗体的筛选%Screening unexpected antibody of red cells blood group in the blood donors

    Institute of Scientific and Technical Information of China (English)

    祝宏; 洪小珍; 吴亚玲; 励晓涛; 马开荣; 兰小飞; 朱发明

    2012-01-01

    目的:分析献血人群中红细胞血型意外抗体的情况,并对抗体特性进行确认.方法:应用O型红细胞结合PK7200血型检测系统筛选红细胞血型意外抗体,利用谱细胞鉴定抗体特性.结果:在献血人群中红细胞血型意外抗体阳性率为0.025%,意外抗体以抗M和冷凝集素为主.发现两例类孟买型血型和两例p血型.结论:献血人群中存在低比例的红细胞血型意外抗体,在献血者血型检测中应加以重视.%Objective;To determine unexpected antibody of red cells blood group in the blood donors and identify the characteristics of the antibody. Methods: The unexpected antibody was screened by 0 blood group cells and PK7200 system. Characteristics of the antibody were identified by panel cells.. Results: The prevalence rate of unexpected antibody was 0.025% in the blood donors. The common unexpected antibodies were anti - M and cold ag-glutinin. Two cases with para - Bombay phenotype and two cases with p phenotype were found. Conclusion; Low prevalence of unexpected antibody existed in the blood donors. It is important to screen unexpected antibody in donor's blood grouping.

  11. Determination of HBV Genotypes among Hbs Ag Positive Blood Donors in Tehran, Iran Using PCR-RFLP

    Directory of Open Access Journals (Sweden)

    S Milani

    2009-03-01

    Full Text Available Background: Hepatitis B virus (HBV is one of the major causative agents of acute and chronic liver disease worldwide and is believed to be responsible for a million deaths annually. On the basis of a comparison of complete genomic se­quences, HBV has been classified into eight genotypes A-H which show a geographical distribution. Some genotypes are associ­ated with different clinical outcomes. Identification of HBV genotypes is important to begin and follow up the treat­ment."nMethods: In this cross-sectional study, the serum samples of blood donors were collected from Tehran Blood Transfusion Cen­ters in period during "2005-2006". Sera of 55 blood donors who were positive for hepatitis B surface antigen were se­lected. DNA was extracted using commercial kit and the S gene sequence was amplified by nested-PCR. PCR products were then analyzed for restriction enzymes that would be genotype specific."nResults: Genotype D was found the only type in all HBV DNA positive serum samples, in Tehran."nConclusion: Genotype D is dominant among Tehran's blood donors, which is consistent with Iran and the Middle East domi­nant genotype.  

  12. Donor blood procurement and the risk of transfusion transmissible viral infections in a tertiary health facility in South-South Nigeria

    Directory of Open Access Journals (Sweden)

    Benedict Nwogoh

    2011-01-01

    Full Text Available Background: Blood and blood products are scarce commodities. The demand often outweighs the supply. This study is directed at investigating the blood procurement sources and the risk of viral transfusion transmissible infection. Materials and Methods: The records of the blood transfusion unit of a tertiary health facility in south-south Nigeria were studied. The procurement and screening records from 1 January to 31 December 2009 were analyzed. Results: 7,552 donor records were analyzed, 6,931 were commercial donor and 621 replacement donors. 891 commercial donors were infected, 500 (7.2% were HIV positive, 323 (4.7% HBV positive, 42 (0.6% had HIV and HBV co-infection, while 28 (0.4% were HCV positive. Twenty-three replacement donors were infected, 16 (2.6% were HIV positive, 6 (1% were HBV positive, while 1 (0.2% were HCV positive. None of the replacement donors had co-infection. The risk of infection was significantly higher with commercial donor procurement (X2=45.07, P<0.001, OD=3.845. Conclusion: Commercial blood donors are still the major source of blood to the hospital and they also have the highest prevalence of transfusion transmissible viral infections in this region thus constitute a major risk transmitting infections to potential recipients.

  13. Controversies in hybrid banking: attitudes of Swiss public umbilical cord blood donors toward private and public banking.

    Science.gov (United States)

    Manegold, Gwendolin; Meyer-Monard, Sandrine; Tichelli, André; Granado, Christina; Hösli, Irene; Troeger, Carolyn

    2011-07-01

    Umbilical cord blood (UCB) stored in public inventories has become an alternative stem cell source for allogeneic stem cell transplantation. The potential use of autologous UCB from private banks is a matter of debate. In the face of the limited resources of public inventories, a discussion on "hybrid" public and private UCB banking has evolved. We aimed to explore the attitudes of the donating parents toward public and private UCB banking. A standardized, anonymous questionnaire was sent to the most recent 621 public UCB donors including items regarding satisfaction with recruitment process, the need for a second consent before release of the UCB unit for stem cell transplantation, and the donors' views on public and private UCB banking. Furthermore, we asked about their views on UCB research. Of the questionnaires, 48% were returned, and 16% were lost due to mail contact. Of our donors, 95% would donate to the public bank again. As much as 35% of them were convinced that public banking was useful. Whereas 27% had never heard about private UCB banking, 34% discussed both options. Nearly 70% of donors opted for public banking due to altruism and the high costs of private banking. Of our public UCB donors, 81% stated that they did not need a re-consent before UCB release for stem cell transplantation. In case of sample rejection, 53.5% wanted to know details about the particular research project. A total of 9% would not consent. Almost all donors would choose public banking again due to altruism and the high costs of private banking. Shortly after donation, mail contact with former UCB donors was difficult. This might be a relevant issue in any sequential hybrid banking.

  14. Computer-assisted audiovisual health history self-interviewing. Results of the pilot study of the Hoxworth Quality Donor System.

    Science.gov (United States)

    Zuck, T F; Cumming, P D; Wallace, E L

    2001-12-01

    The safety of blood for transfusion depends, in part, on the reliability of the health history given by volunteer blood donors. To improve reliability, a pilot study evaluated the use of an interactive computer-based audiovisual donor interviewing system at a typical midwestern blood center in the United States. An interactive video screening system was tested in a community donor center environment on 395 volunteer blood donors. Of the donors using the system, 277 completed surveys regarding their acceptance of and opinions about the system. The study showed that an interactive computer-based audiovisual donor screening system was an effective means of conducting the donor health history. The majority of donors found the system understandable and favored the system over a face-to-face interview. Further, most donors indicated that they would be more likely to return if they were to be screened by such a system. Interactive computer-based audiovisual blood donor screening is useful and well accepted by donors; it may prevent a majority of errors and accidents that are reportable to the FDA; and it may contribute to increased safety and availability of the blood supply.

  15. Unrelated umbilical cord blood transplant for adult acute lymphoblastic leukemia in first and second complete remission: a comparison with allografts from adult unrelated donors

    Science.gov (United States)

    Marks, David I.; Woo, Kwang Ahn; Zhong, Xiaobo; Appelbaum, Frederick R.; Bachanova, Veronika; Barker, Juliet N.; Brunstein, Claudio G.; Gibson, John; Kebriaei, Partow; Lazarus, Hillard M.; Olsson, Richard; Perales, Miguel-Angel; Pidala, Joseph; Savani, Bipin; Rocha, Vanderson; Eapen, Mary

    2014-01-01

    Allogeneic hematopoietic cell transplantation has an established role in the treatment of adults with acute lymphoblastic leukemia whose survival when recipients of grafts from adult unrelated donors approaches that of recipients of grafts from sibling donors. Our aim was to determine the role of mismatched unrelated cord blood grafts in transplantation for 802 adults with acute lymphoblastic leukemia in first or second complete remission. Using Cox regression we compared outcomes after 116 mismatched single or double cord blood transplants, 546 peripheral blood progenitor cell transplants and 140 bone marrow transplants. The characteristics of the recipients and their diseases were similar except cord blood recipients were younger, more likely to be non-Caucasians and more likely to have a low white blood cell count at diagnosis. There were differences in donor-recipient human leukocyte antigen-match depending on the source of the graft. Most adult donor transplants were matched at the allele-level considering human leukocyte antigens-A, -B, -C and –DRB1. In contrast, most cord blood transplants were mismatched and considered antigen-level matching; 57% were mismatched at two loci and 29% at one locus whereas only 29% of adult donor transplants were mismatched at one locus and none at two loci. There were no differences in the 3-year probabilities of survival between recipients of cord blood (44%), matched adult donor (44%) and mismatched adult donor (43%) transplants. Cord blood transplants engrafted slower and were associated with less grade 2–4 acute but similar chronic graft-versus-host disease, relapse, and transplant-related mortality. The survival of cord blood graft recipients was similar to that of recipients of matched or mismatched unrelated adult donor grafts and so cord blood should be considered a valid alternative source of stem cells for adults with acute lymphoblastic leukemia in the absence of a matched unrelated adult donor. PMID:24056817

  16. Prevention of pure red cell aplasia after major or bidirectional ABO blood group incompatible hematopoietic stem cell transplantation by pretransplant reduction of host anti-donor isoagglutinins

    OpenAIRE

    Stussi, G.; Halter, J; Bucheli, E; Valli, P V; Seebach, L; Gmür, J; Gratwohl, A; Passweg, J. R.; Seebach, J.D.

    2009-01-01

    BACKGROUND: Persistent anti-donor isoagglutinins after major ABO blood group incompatible hematopoietic stem cell transplantation may cause delayed red blood cell engraftment and post-transplant pure red cell aplasia. DESIGN AND METHODS: We investigated the effect of pretransplant anti-donor isoagglutinin reduction by in vivo absorption and/or plasmapheresis on the incidence of pure red cell aplasia and the time to red blood cell engraftment in 153 hematopoietic stem cell transplant recipient...

  17. Bacterial contamination of blood components: Norwegian strategies in identifying donors with higher risk of inducing septic transfusion reactions in recipients.

    Science.gov (United States)

    Klausen, Sofie Strand; Hervig, Tor; Seghatchian, Jerard; Reikvam, Håkon

    2014-10-01

    Bacterial contamination of blood and its cellular components remains the most common microbiological cause of transfusion associated morbidity and mortality, even in developed countries. This yet unresolved complication is seen more often in platelet transfusions, as platelet concentrates are stored at room temperature, in gas permeable containers with constant agitation, which support bacterial proliferation from relatively low undetectable levels, at the beginning of storage time, to relatively high virulent bacteria titers and endotoxin generation, at the end of shelf life. Accordingly, several combined strategies are introduced and implemented to at least reduce the potential risk of bacterial contaminated products for transfusion. These embody: improved donors arms cleaning; bacterial avoidance by diversion of the first portion of collection; reducing bacterial growth through development of newer storage media for longer platelet shelf life; bacterial load reduction by leucoreduction/viral inactivation, in some countries and eliminating the use potentially contaminated units through screening, through current available testing procedures, though none are not