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Sample records for volli kalm ene

  1. Kes peaks tagama seaduste täitmise? / Inge Timoštšuk, Volli Kalm, Maiki Udam ; üles kirjutanud Ülo Tikk

    Index Scriptorium Estoniae

    Timoštšuk, Inge, 1970-

    2001-01-01

    Vene koolidel käib Põhikooli- ja Gümnaasiumiseaduse § 52 teise lõike "Riigi- ja munitsipaalgümnaasiumides alustatakse üleminekut eesti õppekeelele hiljemalt 2007/08. õppeaastal" täitmine üle jõu, kuna ei jätku pädevaid õpetajaid. Teemat kommenteerivad Tartu Ülikooli prorektor Volli Kalm, Tallinna Pedagoogikaülikooli õpetajakoolituse osakonna juhataja Inge Timoštšuk ja Haridusministeeriumi kõrghariduse talituse juhataja Maiki Udam

  2. Mart Kalmu elutöö valmis / Mart Kalm ; interv. Ene Läkk

    Index Scriptorium Estoniae

    Kalm, Mart, 1961-

    2002-01-01

    Arhitektuuriajaloolane Mart Kalm räägib oma tööst ja äsjailmunud raamatust "Eesti 20. sajandi arhitektuur". Karin Hallase ja Triin Ojari repliigid Mart Kalmust, Mart Kalmu biograafilised lühiandmed

  3. Siinmaa maja õppetunde / Mart Kalm

    Index Scriptorium Estoniae

    Kalm, Mart, 1961-

    2007-01-01

    Pärnus Rüütli 1a asuva linnaarhitekt Olev Siinmaa villa (1931-1933), mille 1997. a. ostis ärimees Toomas Truuvert, restaureerimisest. Muinsuskaitse eritingimused, muinsuskaitseline järelevalve: Mart Kalm. Uuringud: Marika Kätsi. Tehniline projekt: Indrek Reino. Sisearhitektuurne projekt: Taso Mähar. Säilinud sisseehitatud mööbli restaureeris Ado Talimaa firma. 5 vaadet

  4. Ene Ammer / Ene Ammer

    Index Scriptorium Estoniae

    Ammer, Ene

    1998-01-01

    ARS-i disainer Ene Ammer õppimisest Tartu kunstikoolis, ERKI-s. Esimestest töödest ARS-is, esimestest näitusekujundustest (elektroonika-alane autorong rändnäitus Moskva tellimisel), personaalnäituste ja messide kujundustest, suurnäituste komplekskujundusest, Olümpianäitusest, viimastest suurnäitustest pikemalt 1988. a. korraldatud Baltikumi ühisnäitusest "Kõik laste heaks" kujundusest. Sisearhitekt Mait Summatavet Ene Ammerist

  5. Ka Eesti vajab oma pildiraamatuid / Anu Kalm

    Index Scriptorium Estoniae

    Kalm, Anu, 1960-

    2007-01-01

    Bologna lasteraamatumessist. IBBY (The International Board on Books for Young People) Eesti esindajatena olid messil Piret Raud ja Anu Kalm. Piret Raua tööd olid valitud rahvusvahelisele illustraatorite näitusele, mille aukülaline oli saksa illustraator Wolf Erlbruch. Eestit esindasid kolm raamatut IBBY rahvusvahelisel stendil. Kümme parimat raamatut ja viimase 40 aasta parimad raamatud olid eksponeeritud Bologna Ragazzi Award'i ülikooli raamatukogus

  6. Ene Ergma / Ene Ergma ; interv. Anneli Ammas

    Index Scriptorium Estoniae

    Ergma, Ene, 1944-

    2007-01-01

    Eesti Vabariigi Riigikogu esimees 2003-2006 Ene Ergma oma lapsepõlvest ja elukutsevalikust, Moskva aastaist, isiksustest teaduses ja poliitikas, poliitilise karjääri algusest ja erakonna valikust, naistest poliitikas

  7. Kool ja kultuur / Eduard Ubaleht, Kersti Liivak, Õile Kalme, Helen Lauk

    Index Scriptorium Estoniae

    2002-01-01

    Albu mõisahoones asuvast Albu Põhikoolist räägivad kooli direktor Eduard Ubaleht, kunstiõpetaja Kersti Liivak, kooli endine direktor, algklassiõpetaja Õile Kalme ja kooli vilistlane Helen Lauk / vahendas Aime Jaagus

  8. Nõukogude modernism : perifeeria rääkimata lood / Mart Kalm ; intervjueerinud Sille Pihlak

    Index Scriptorium Estoniae

    Kalm, Mart, 1961-

    2012-01-01

    Näitus "Nõukogude modernism 1955-1991. Rääkimata lood" Viini Arhitektuurikeskuses. Kuraatorid: Katharina Ritter, Ekaterina Shapiro-Obermair, Alexandra Wachter. Näitusega kaasneb publikatsioon. Näituselt puudub Venemaa. Arhitektuuriajaloolane Mart Kalm eesti arhitektuurist ja oma ettekandest konverentsil

  9. Žene Frankopanke

    OpenAIRE

    Šercer, Marija

    2011-01-01

    Izabravši četrnaest žena koje su rođene kao Frankopanke, ili su udajom dospjele u obitelj Frankopan autorica je ukazala na uvjete u kojima su se ženska djeca udavala. Njihova dob od devet ili dvanaest godina, nedovoljno poznavanje budućega supruga, nepoznavanje sredine u koju su ih udavali, možebitno nepoznavanje jezika, otežavali su život toj nezreloj djeci. Temeljna zadaća ženskoga djeteta bila je rađanje djece i osiguravanje potomstva. Česti porodi su nerijetko dovodili do prerane smrti. T...

  10. Olev Siinmaa elamu (Rüütli 1a, Pärnu) restaureerimine. / Olev Siinmaa, Mart Kalm, Aado Talimaa

    Index Scriptorium Estoniae

    Siinmaa, Olev, 1881-1948

    2007-01-01

    Välisvaade (foto: Vallo Kruuser), sisevaade enne (Eesti Arhitektuurimuusem) ja pärast restaureerimist (foto: Mart Kalm). Omanik Toomas Truuvert. EK arhitektuuri sihtkapitali 2006. a. parima restaureeritud objekti preemia

  11. Aasta tekstiilikunstnikuks valiti Ene Pars

    Index Scriptorium Estoniae

    2011-01-01

    Eesti Tekstiilikunstnike Liit valis aasta tekstiilikunstnikuks Ene Parsi. Aasta teo 2010 preemia vääriliseks tunnistati Kadri Viires. Preemiakandidaatide näitus avatakse 18. märtsil Tallinnas Hop galeriis

  12. Microbial synthesis of alka(enes

    Directory of Open Access Journals (Sweden)

    Weihua eWang

    2013-10-01

    Full Text Available Alka(enes are the predominant constituents of gasoline, diesel, and jet fuels. They can be produced naturally by a wide range of microorganisms. Bio- alka(enes can be used as drop-in biofuels. To date, five microbial pathways that convert free fatty acids or fatty acid derivatives into alka(enes have been identified or reconstituted. The discoveries open a door to achieve microbial production of alka(enes with high efficiency. The modules derived from these alka(ene biosynthetic pathways can be assembled as biological parts and synthetic biology strategies can be employed to optimize the metabolic pathways and improve alka(ene production.

  13. Ene Sepp esitles esikteost "Medaljon" / Heli Talinurm

    Index Scriptorium Estoniae

    Talinurm, Heli

    2009-01-01

    Ene Sepp esitles 19. veebruaril Rapla Keskraamatukogus oma raamatut, mis pälvis kolmanda koha Eesti Lastekirjanduse Teabekeskuse ja kirjastuse Tänapäev 2008. aasta noorsooromaani võistlusel. Ly Ehini mõtteid, mida Ene Sepa raamat temas tekitas

  14. Fitopreparati za žene u menopauzi

    OpenAIRE

    Peteljak, Ivana

    2015-01-01

    Menopauza predstavlja kraj fizioloških menstrualnih krvarenja, odnosno kraj reproduktivne dobi žene. Očituje se brojnim vazomotornim smetnjama i psihičkim promjenama. U želji da ublaže simptome menopauze žene posežu za hormonskom nadomjesnom terapijom, a u zadnje vrijeme i brojnim biljnim pripravcima. Ovaj rad daje pregled biljnih vrsta za koje se smatra da bi mogle poslužiti kao alternativa estrogenima za olakšanje simptoma menopauze. Znanstvena istraživanja su pokazala da je biljna vrsta Ci...

  15. preparation of bicyclic lactones using lewis acids catalyzed ene ...

    African Journals Online (AJOL)

    Administrator

    INTRODUCTION. The synthesis of the cis-fused bicyclic lactones relies extensively on the Lewis acid catalysed carbonyl-ene reaction which has been widely used to access carbocycles. In 1943 Alder discovered the ene-reaction and classified it in his Nobel Lecture as an “indirect substitution addition” or “ene synthesis" in ...

  16. ENES the European Network for Earth System modelling and its infrastructure projects IS-ENES

    Science.gov (United States)

    Guglielmo, Francesca; Joussaume, Sylvie; Parinet, Marie

    2016-04-01

    The scientific community working on climate modelling is organized within the European Network for Earth System modelling (ENES). In the past decade, several European university departments, research centres, meteorological services, computer centres, and industrial partners engaged in the creation of ENES with the purpose of working together and cooperating towards the further development of the network, by signing a Memorandum of Understanding. As of 2015, the consortium counts 47 partners. The climate modelling community, and thus ENES, faces challenges which are both science-driven, i.e. analysing of the full complexity of the Earth System to improve our understanding and prediction of climate changes, and have multi-faceted societal implications, as a better representation of climate change on regional scales leads to improved understanding and prediction of impacts and to the development and provision of climate services. ENES, promoting and endorsing projects and initiatives, helps in developing and evaluating of state-of-the-art climate and Earth system models, facilitates model inter-comparison studies, encourages exchanges of software and model results, and fosters the use of high performance computing facilities dedicated to high-resolution multi-model experiments. ENES brings together public and private partners, integrates countries underrepresented in climate modelling studies, and reaches out to different user communities, thus enhancing European expertise and competitiveness. In this need of sophisticated models, world-class, high-performance computers, and state-of-the-art software solutions to make efficient use of models, data and hardware, a key role is played by the constitution and maintenance of a solid infrastructure, developing and providing services to the different user communities. ENES has investigated the infrastructural needs and has received funding from the EU FP7 program for the IS-ENES (InfraStructure for ENES) phase I and II

  17. 968-IJBCS-Article-Ene Pascal Chuka

    African Journals Online (AJOL)

    T60

    indexmedicus.afro.who.int. Phenotypic assay of adherent E. coli strains using hep-2 cells on diarrheic children in Rivers State of Nigeria. Pascal Chuka ENE1*, Sam D. ABBEY1, Chukwudi Gregory MADUBUATTAH2 and. Chukwubuike Udoka OKEKE3.

  18. Tipparhitektuur tagasi kinnisvaraturul / Ene Läkk

    Index Scriptorium Estoniae

    Läkk, Ene, 1957-

    2008-01-01

    Müüki on pandud Ralf Tamme projekteeritud kuubikmaja Veskimöldres, Vahur Sova funkmaja Pirital, Kalle Rõõmuse eramu Merirahu elamurajoonis, Ene Priimetsa paarismaja Nõmmel, Mart Liho projekteritud maja Nõmmel. Ralf Tamme ja Tõnu Toomparki kommentaarid. Fotod elamutest

  19. Kandidaadid : Ene Ergma versus Toomas Hendrik Ilves

    Index Scriptorium Estoniae

    2006-01-01

    Artiklis vastatakse küsimustele, kes oleksid Kadriorus Ene Ergma ja Toomas Hendrik Ilvese nõunikud, millist sõnumit kannaks nende esimene iseseisvuspäeva kõne, kuidas nad tähistaksid oma võitu presidendivalimistel, kellele nad annaksid Maarjamaa Risti, kuhu nad teeksid oma esimese välisvisiidi

  20. Feminizam ili poslanje žene?

    OpenAIRE

    Mišić, Anto

    2005-01-01

    Premda ni feminizam ni poslanje žene nisu tipično filozofske (metafizičke) teme, filozofsko promišljanje pomaže jasnijem poimanju, žene, njezina odnosa i uloge u društvu. Bez ispravnog shvaćanja ljudske naravi, a ona jednako pripada ženi i muškarcu, lako se upada u jednostranost i isključivost stavova i djelovanja. Premda ljudska narav, osobnost, jednako pripada i muškarcu i ženi, ona se u njima ipak različito ostvaruje. Zbog individualnih razlika, ljudska se narav na različite načine izriče,...

  1. IS-ENES project management - lessons learnt

    Science.gov (United States)

    Parinet, Marie; Guglielmo, Francesca; Joussaume, Sylvie

    2017-04-01

    IS-ENES is the distributed e-infrastructure of models, model data and metadata of the European Network for Earth System Modelling (ENES). It has benefitted from two EC FP7 grants and aims towards further European and national funding to achieve sustainability. We highlight here several challenges related to project management that have risen in the course of these two project-phases spanning 8 years. Some challenges are related to the heterogeneity of the activities within IS-ENES, with different groups working on very diverse activities, not necessarily strictly interdependent. An immediate consequence is the need of implementing and setting up in early phases of the project efficient collection and circulation of information to preserve and reinforce the systemic view of the infrastructure as a whole and the pursuit of common goals, including coordinated provision of services. Toward and beyond such common goals, managing IS-ENES, covering both scientific and more strictly management-related aspects, implies a double-paced approach: besides setting up efficient project workflow, there is the need of setting up longer term objectives. This implies, within the project lifetime, to elaborate and implement a coherent organizational (consistent with scientific goals, funding schemes, research and technology landscape) strategy to pursue these goals beyond the project itself. Furthermore, a series of more generic project management challenges will also be listed and can be gathered around 3 main objectives: ease the internal processes in order to optimize the work, anticipate delays and budget issues, and motivate the project teams by ensuring an efficient internal and external communication.

  2. Sa uurisid ekspressionismi 1960ndatel ja 70ndatel... / Ene Lamp

    Index Scriptorium Estoniae

    Lamp, Ene

    2005-01-01

    2004. a. ilmunud raamatu "Ekspressionism" eest Eesti Kultuurkapitali suure kunstipreemia (100000 kr.) saanud Ene Lamp ekspressionismi tähenduse muutumiset, ekspressionismi rollist eesti kunstis, oma tulevikuplaanidest

  3. Ene Ergma : riigikogu liikme töökoht on riigikogus / Ene Ergma

    Index Scriptorium Estoniae

    Ergma, Ene, 1944-

    2007-01-01

    Riigikogu esimees vastab EPL Online'i lugejate küsimustele, mis muuhulgas puudutavad ka president Toomas Hendrik Ilvese ideed muuta kaitseväe juhataja ametisse määramise korda. Ene Ergma kiidab ka presidendi kõnet Riigikogu XI koosseisu avaistungil

  4. Ene-Liis Semper kodustab ruume / Ene-Liis Semper ; intervjueerinud Liina Luhats

    Index Scriptorium Estoniae

    Semper, Ene-Liis, 1969-

    2011-01-01

    Video- ja teatrikunstnik Ene-Liis Semper oma Kumu Kunstimuuseumis 31. dets.-ni 2011 avatud näitusest, mis räägib ajast ja ajalikkusest ning inimesekolemisest selles, oma loomingust, valdkondade vahel liikumisest, tööst NO99 teatris, loomingulistest plaanidest

  5. Thiol-Ene Networks for Photon Upconversion

    Science.gov (United States)

    Lott, Joseph; Jentsch, Abagail

    Photon upconversion via the mechanism of triplet-triplet annihilation (TTA) offers the prospect of employing low-power and non-coherent excitation sources to drive the upconversion process. Solid-state materials capable of TTA present several significant challenges including limited solubility of the required chromophores and reduced molecular diffusion. Owing to the wide range of chemical compositions, tunable mechanical properties, and the ease of processing, polymeric materials have been at the forefront of solid-state materials for TTA. This study focuses on the use of thiol-ene `click' chemistry to create elastomeric films for TTA. Functionalized derivatives of 9,10-diphenylanthracene bearing two vinyl groups were synthesized and covalently incorporated into thiol-ene films, along with palladium (II) octaethylporphine as a sensitizing chromophore. Both emitter and sensitizer concentrations were systematically varied. The network formation was monitored using FTIR measurements and leaching experiments were conducted to quantify the amount of emitter chromophores not bonded into the network. The thermal and mechanical properties of the films were characterized using TGA, DSC, and DMTA. The upconverting performance was gauged using steady-state photoluminescence and lifetime measurements.

  6. An academic with ambition / Ene Ergma ; interv. Kadri Kukk

    Index Scriptorium Estoniae

    Ergma, Ene, 1944-

    2006-01-01

    Intervjuus Riigikogu asespiikriga käsitletakse kõrghariduse korraldust, professorite rohkust kõrgkoolides, võimalikku kandideerimist Tartu ülikooli rektori kohale, omandatud poliitilisi kogemusi ning ajude äravoolu Eestist. Ene Ergma CV

  7. 5-Ene-4-thiazolidinones - An efficient tool in medicinal chemistry.

    Science.gov (United States)

    Kaminskyy, Danylo; Kryshchyshyn, Anna; Lesyk, Roman

    2017-11-10

    The presented review is an attempt to summarize a huge volume of data on 5-ene-4-thiazolidinones being a widely studied class of small molecules used in modern organic and medicinal chemistry. The manuscript covers approaches to the synthesis of 5-ene-4-thiazolidinone derivatives: modification of the C5 position of the basic core; synthesis of the target compounds in the one-pot or multistage reactions or transformation of other related heterocycles. The most prominent pharmacological profiles of 5-ene derivatives of different 4-thiazolidinone subtypes belonging to hit-, lead-compounds, drug-candidates and drugs as well as the most studied targets have been discussed. Currently target compounds (especially 5-en-rhodanines) are assigned as frequent hitters or pan-assay interference compounds (PAINS) within high-throughput screening campaigns. Nevertheless, the crucial impact of the presence/nature of C5 substituent (namely 5-ene) on the pharmacological effects of 5-ene-4-thiazolidinones was confirmed by the numerous listed findings from the original articles. The main directions for active 5-ene-4-thiazolidinones optimization have been shown: i) complication of the fragment in the C5 position; ii) introduction of the substituents in the N3 position (especially fragments with carboxylic group or its derivatives); iii) annealing in complex heterocyclic systems; iv) combination with other pharmacologically attractive fragments within hybrid pharmacophore approach. Moreover, the utilization of 5-ene-4-thiazolidinones in the synthesis of complex compounds with potent pharmacological application is described. The chemical transformations cover mainly the reactions which involve the exocyclic double bond in C5 position of the main core and correspond to the abovementioned direction of the 5-ene-4-thiazolidinone modification. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  8. Hexaarylbiimidazoles as Visible Light Thiol–Ene Photoinitiators

    Science.gov (United States)

    Clarkson, Brian H.; Scott, Timothy F.

    2015-01-01

    Objectives The aim of this study is to determine if hexaarylbiimidazoles (HABIs) are efficient, visible light-active photoinitiators for thiol–ene systems. We hypothesize that, owing to the reactivity of lophyl radicals with thiols and the necessarily high concentration of thiol in thiol–ene formulations, HABIs will effectively initiate thiol–ene polymerization upon visible light irradiation. Methods UV-vis absorption spectra of photoinitiator solutions were obtained using UV-vis spectroscopy, while EPR spectroscopy was used to confirm radical species generation upon HABI photolysis. Functional group conversions during photopolymerization were monitored using FTIR spectroscopy, and thermomechanical properties were determined using dynamic mechanical analysis. Results The HABI derivatives investigated exhibit less absorptivity than camphorquinone at 469 nm; however, they afford increased sensitivity at this wavelength when compared with bis(2,4,6-trimethylbenzoyl)-phenylphosphineoxide. Photolysis of the investigated HABIs affords lophyl radicals. Affixing hydroxyhexyl functional groups to the HABI core significantly improved solubility. Thiol–ene resins formulated with HABI photoinitiators polymerized rapidly upon irradiation with 469 nm. The glass transition temperatures of the thiol–ene resin formulated with a bis(hydroxyhexyl)-functionalized HABI and photopolymerized at room and body temperature were 49.5±0.5°C and 52.2±0.1°C, respectively. Significance Although thiol–enes show promise as continuous phases for composite dental restorative materials, they show poor reactivity with the conventional camphorquinone/tertiary amine photoinitiation system. Conversely, despite their relatively low visible light absorptivity, HABI photoinitiators afford rapid thiol–ene photopolymerization rates. Moreover, minor structural modifications suggest pathways for improved HABI solubility and visible light absorption. PMID:26119702

  9. The diagenetic fate of taraxer-14-ene and oleanene isomers

    Energy Technology Data Exchange (ETDEWEB)

    Ten Haven, H.L.; Rullkoetter, J. (Institute of Petroleum and Organic Geochemistry, Juelich (West Germany))

    1988-10-01

    The extractable organic matter in Holocene to early Miocene deep-sea sediments from Site 645 (ODP Leg 105) in Baffin Bay contains almost exclusively biological markers of terrigenous origin. Pentacyclic triterpenoids of the {alpha}-(ursene) and {beta}-amyrin (oleanene) type often occur as the most abundant compounds in the aliphatic hydrocarbon, ketone and alcohol fractions of the sediment extract. A specific diagenetic reaction involves the conversion of taraxer-14-ene into olean-12-ene in the upper 700 meters of the sedimentary sequence. The taraxerene-to-oleanene conversion is complete before the onset of isomerization of diasterenes at C-20. In the sediment as well as in laboratory simulation experiments, olean-12-ene further isomerizes to olean-13(18)-ene and olean-18-ene, the latter of which may be the direct precursor of 18{beta}(H)- and 18{alpha}(H)-oleanane found in sediments containing more mature organic matter and in crude oils. The subsurface interconversion of these triterpenoid skeletons indicate that oleanane does not necessarily start life as an oleanoid.

  10. Ene Ergma kihutas Valka 120 kilomeetrit tunnis / Kadi Viljak, Siim Randla

    Index Scriptorium Estoniae

    Viljak, Kadi

    2003-01-01

    Riigikogu spiiker Ene Ergma kasutas Valga kultuurikeskusesse valijatega kohtumisele jõudmiseks ametiautol vilkurit, mis lubab hoida kiirust vähemalt 120 kilomeetrit tunnis. Novembrikuus on Ene Ergma autojuht registreerinud alarmsõitu kaheksal korral

  11. Kilde Hiiumaa maleajaloost / Volli Mäeumbaed

    Index Scriptorium Estoniae

    Mäeumbaed, Volli

    1999-01-01

    Järgneb 19.,26. nov., 3.,10.,17.,24.,31. dets. ; 2000, 7.,14.,21.,28. jaan, 4.,11.,18.,26. veebr., 3.,10.,17.,24.,31. märts, 7.,14.,20.,28. apr., 5.,12.,19.,26. mai, 2.,9.,16.,22. juuni, 28. juuli, 4.,11.,25. aug., 1.,8.,15.,22. sept., 20.,27. okt.,3., 10.,17.,24. nov., 1.,8.,15.,22.,29.dets. ; 2001, 19.,26. jaan., 2.,9.,16.,23. veebr., 2.,9.,16.,23.,30. märts, 6.,12.,20.,27. apr., 4.,11. mai

  12. Volli - Eesti parim õpilasfirma 2006 / Pille Rõivas

    Index Scriptorium Estoniae

    Rõivas, Pille

    2006-01-01

    2006. aasta parima õpilasfirma valimisest, mille võitis Tallinna 21. Kool. Võitjad osalevad ka Šveitsis toimuvatel Euroopa riikide õpilasfirmade võistlusel "Junior Achievement - Young Enterprice Europe". Vt. samas: Esikohale tihe rebimine. Žürii: Oluline on idee edasiarendus.

  13. Ene ja Tõnis Kask pole valikuga rahul / Tiit Tuumalu

    Index Scriptorium Estoniae

    Tuumalu, Tiit, 1971-

    2003-01-01

    Eesti Filmiajakirjanike Ühing andis auhinna Aasta film 2002 mängufilmile "Agent Sinikael" : režissöör Marko Raat. Viimane sai preemiaks 12 000 krooni. Auhinna rahastajad omanimelise fondi kaudu Eesti Rahvuskultuuri Fondi juures Ene ja Tõnis Kask peavad "Nimed marmortahvlil" väljajätmist isegi nominentide hulgast shokeerivaks

  14. [Raamat] / Elina Kalm

    Index Scriptorium Estoniae

    Kalm, Elina

    2007-01-01

    Tutvustus: Stroud, Jonathan. Samarkandi amulett / tlk. Bibi Raid. Tallinn : Pegasus, 2005 ; Stroud, Jonathan. Golemi silm / tlk. Bibi Raid. Tallinn : Pegasus, 2007 ; Stroud, Jonathan. Ptolemaiose värav / tlk. Bibi Raid. Tallinn : Pegasus, 2007. (Bartimaeuse triloogia ; 1-3)

  15. Evidence for distinct dehydrogenase and isomerase sites within a single 3. beta. -hydroxysteroid dehydrogenase/5-ene-4-ene isomerase protein

    Energy Technology Data Exchange (ETDEWEB)

    Luu-The, V.; Takahashi, Masakazu; de Launoit, Y.; Dumont, M.; Lachance, Y.; Labrie, F. (Laval Univ., Quebec City, Quebec (Canada))

    1991-09-10

    Complementary DNA encoding human 3{beta}-hydroxysteroid dehydrogenase/5-ene-4-ene isomerase (3-{beta}-HSD) has been expressed in transfected GH{sub 4}C{sub 1} with use of the cytomegalovirus promoter. The activity of the expressed protein clearly shows that both dehydrogenase and isomerase enzymatic activities are present within a single protein. However, such findings do not indicate whether the two activities reside within one or two closely related catalytic sites. With use of ({sup 3}H)-5-androstenedione, the intermediate compound in dehydroepiandrosterone (DHEA) transformation into 4-androstenedione by 3{beta}-HSD, the present study shows that 4MA (N,N-diethyl-4-methyl-3-oxo-4-aza-5{alpha}-androstane-17{beta}-carboxamide) and its analogues of 5-androstenedione to 4-androstenedione with an approximately 1,000-fold higher K{sub i} value. The present results thus strongly suggest that dehydrogenase and isomerase activities are present at separate sites on the 3-{beta}-HSD protein. Such data suggest that the irreversible step in the transformation of DHEA to 4-androstenedione is due to a separate site possessing isomerase activity that converts the 5-ene-3-keto to a much more stable 4-ene-3-keto configuration.

  16. Ene Ergma ja Toomas Hendrik Ilves : presidendikampaania kipub palaganiks / Ene Ergma, Toomas Hendrik Ilves ; interv. Kalev Vilgats

    Index Scriptorium Estoniae

    Ergma, Ene, 1944-

    2006-01-01

    Presidendikandidaadid Ene Ergma ja Toomas Hendrik Ilves annavad omapoolsed hinnangud eelolevatele presidendivalimistele. Vt. samas: Eesti Vabariigi presidendi valimise kord. Vt. ka Riina Martinsoni lühiinterv. Riigikogu liikmete Koit Pikaro, Mark Soosaare, Jaanus Männiku, Väino Linde, Trivimi Velliste, Toomas Alatalu, Aivar Õuna ja Ela Tomsoniga

  17. Ene Ergma : ikka otsitakse ju heledate lokkis juustega tüdrukuid / Ene Ergma ; interv. Märt Kivine

    Index Scriptorium Estoniae

    Ergma, Ene, 1944-

    2006-01-01

    Res Publica presidendikandidaadi sõnul on Eesti presidendi roll olla Eesti riigi nägu väljapoole. Presidendil on hiiglaslik vastutus, mitte niivõrd palju võimu, ta peab tajuma, kus on ühiskonna valulävi. CV: Ene Ergma

  18. 5 küsimust Ene Läkile / Ene Läkk ; interv. Ell-Maaja Randküla

    Index Scriptorium Estoniae

    Läkk, Ene, 1957-

    2006-01-01

    Ene Läkist, kes on alates 2005. aastast interjööri-, disaini- ja arhitektuuriajakirja "Ruum" peatoimetaja. Ene Läkk, Eesti Sisearhitektide Liidu 2005. a. aastapreemia nominent ajakirjandusliku tegevuse eest, ajakirjast "Ruum". Ill.: 6 värv. vaadet, foto ajakirjanikust

  19. Teekond kogunemisest koostööni / Ene Kulasalu, Ene Velström, Koidu Tani-Jürisoo, Mari Karm ; vahendas Mari Karm

    Index Scriptorium Estoniae

    2003-01-01

    Vestlusringis on Avatud Meele Instituudi koolitajad Ene Kulasalu, Ene Velström, Koidu Tani-Jürisoo ja Mari Karm, et arutada koostöö ja selle juhtimise võimalikkust ja vajalikkust, grupi arengufaaside (sõltuvusfaas, konfliktifaas, eraldumisfaas, koostööfaas) toimimist ja nende teadvustamise tähtsust

  20. Ene-Liiz Semper. Anton Litvin. Bogdan Mamonov

    Index Scriptorium Estoniae

    2005-01-01

    Veebruaris Madridis toimunud Euroopa kunstilaada ARCO jaoks Vene saatkonna galerii poolt koostatud kunstinäitus TRINITY toimub nüüd Tallinnas Vene saatkonna galeriis. Kuraatorid Masha ja Andrei Melnikovid. Osalevad Ene-Liis Semper videoga ArtVideo, vene kunstnik Bogdan Mamonov maaliseeriaga "Ma kanoniseerisin oma sõpru" ja vene kunstnik Anton Litvin fotoprojektiga "Punktuatsioon". Madridis näitas Semper videot "Nimetu"

  1. Functional Conducting Polymers via Thiol-ene Chemistry

    OpenAIRE

    Feldman, Kathleen E.; Martin, David C.

    2012-01-01

    We demonstrate here that thiol-ene chemistry can be used to provide side-chain functionalized monomers based on 3,4-propylenedioxythiophene (ProDOT) containing ionic, neutral, hydrophobic, and hydrophilic side chains. All reactions gave high yields and purification could generally be accomplished through precipitation. These monomers were polymerized either chemically or electro-chemically to give soluble materials or conductive films, respectively. This strategy provides for facile tuning of...

  2. Multi-chamber and multi-layer thiol-ene microchip for cell culture

    DEFF Research Database (Denmark)

    Tan, H. Y.; Hemmingsen, Mette; Lafleur, Josiane P.

    2014-01-01

    We present a multi-layer and multi-chamber microfluidic chip fabricated using two different thiol-ene mixtures. Sandwiched between the thiol-ene chip layers is a commercially available membrane whose morphology has been altered with coatings of thiol-ene mixtures. Experiments have been conducted ...... with the microchip and shown that the fabricated microchip is suitable for long term cell culture....

  3. IS-ENES: The Infrastructure for the European Network for Earth System modelling

    Science.gov (United States)

    Joussaume, Sylvie

    2013-04-01

    IS-ENES is the distributed e-infrastructure of models, model data and metadata of the European Network for Earth System Modelling (ENES). This network gathers together the European climate modelling community working on understanding and predicting climate variability and change. It organizes and supports European contributions to international experiments used in assessments of the Intergovernmental Panel on Climate Change, such as the Fifth Coupled Model Intercomparison Project (CMIP5) and the Coordinated regional Climate Downscaling Experiments (CORDEX). IS-ENES is supported by FP7 and is entering its second 4-year phase (http://is.enes.org). IS-ENES integrates the European climate modelling community, stimulates common developments of software for models and their environments, fosters the execution and exploitation of high-end simulations, in particular using the European PRACE facilities, and supports the dissemination of model results to the climate research and climate impact communities. The central point of entry to IS-ENES services, the ENES Portal (http://enes.org), integrates information on the European climate models and provides access to models and software environments needed to run and exploit model simulations, such as the climate data operators, as well as to simulation data, metadata and processing utilities. IS-ENES supports the European contribution to the international Earth System Grid Federation data distribution for CMIP5 and CORDEX. In order to help the use of climate model results for impact studies, e.g. in water management, a prototype portal climate4impact (http://www.climate4impact.eu) has been developed providing access to guidance on how to use global climate model results through documentation of use cases, and will be further developed in IS-ENES2. Adaptation to climate change requires strengthening the integration between the climate and hydro-meteorological communities and the e-infrastructure IS-ENES2 might contribute to this

  4. Naknada štete prouzročene kaznenim djelom

    OpenAIRE

    Votić, Antonija

    2016-01-01

    Tema ovog završnog rada je naknada štete prouzročene kaznenim djelom. U tom radu autorica je obuhvatila sljedeća poglavlja; opće i posebne pretpostavke odgovornosti za štetu, subjekte obveznog odnosa odgovornosti za štetu, nadalje vrste odgovornosti, vlastitu odgovornost, odgovornost za drugoga i odgovornost više osoba za istu štetu zatim je objasnila posebne slučajeve odgovornosti, popravljanje štete te uklanjanje opasnosti štete. Autorica je posebnu pozornost pridodala poglavljima o kazn...

  5. Fabrication of a microfluidic cell made of thiol-ene for microarray applications

    DEFF Research Database (Denmark)

    Sola, Laura; Gagni, Paola; Jensen, Thomas G.

    2014-01-01

    We report on the fabrication of a microfluidic cell completely made of thiol-ene for microarray applications. To overcome the issues related to the hydrophobic nature of the thiol-ene material we have also introduced a new polymeric coating based of N, N-dimethylacrylamide (DMA) which is able to ...

  6. Novel Thiol-Ene Hybrid Coating for Metal Protection

    Directory of Open Access Journals (Sweden)

    Mona Taghavikish

    2016-04-01

    Full Text Available A novel hybrid anticorrosion coating with dual network of inorganic (Si–O–Si and organic bonds (C–S–C was prepared on metal through an in situ sol-gel and thiol-ene click reaction. This novel interfacial thin film coating incorporates (3-mercaptopropyl trimethoxysilane (MPTS and 1,4-di(vinylimidazolium butane bisbromide based polymerizable ionic liquid (PIL to form a thiol-ene based photo-polymerized film, which on subsequent sol-gel reaction forms a thin hybrid interfacial layer on metal surface. On top of this PIL hybrid film, a self-assembled nanophase particle (SNAP coating was employed to prepare a multilayer thin film coating for better corrosion protection and barrier performance. The novel PIL hybrid film was characterised for structure and properties using Fourier transform infrared spectroscopy (FTIR, differential scanning calorimetry (DSC, and thermogravimetric analysis (TGA. The corrosion protection performance of the multilayer coating was examined using potentiodynamic polarization and electrochemical impedance spectroscopy (EIS. The results reveal that this novel double layer coating on metal offers excellent protection against corrosion and has remarkably improved the barrier effect of the coating.

  7. Sobre el Kitāb al-garīb al-muntaqà min kalm ahl al-tuqà de Ibn Jamīs de Évora, atribuido a Ibn Masarra

    Directory of Open Access Journals (Sweden)

    Garrido, Pilar

    2009-12-01

    Full Text Available Recently, a copy of the until now unavailable book al-garīb al-muntaqà min kalm ahl al-tuqà has been found. In the manuscript, its authorship is attributed to Ibn Masarra. This article shows that its more plausible author is, according to the sources that mention this title, Ibn Khamīs al-Jāburī, an important link in the history of Andalusian Sufism. The structure and content of this ascetic and mystical work which Ibn Khamīs transmitted to the famous qāḍī ‘Iyāḍ are also studied here.

    Recientemente se ha encontrado una copia de la obra al-garīb al-muntaqà min kalm ahl al-tuqà, hasta ahora desaparecida. En el manuscrito la autoría del Muntaqà se atribuye a Ibn Masarra. En este artículo se muestra que su más probable autor, según indican las fuentes, es Ibn Jamīs al-Yāburī, importante eslabón en la historia del sufismo andalusí. Se analizan además la estructura y el contenido de esta obra ascético-mística que Ibn Jamīs transmitió al cadí ‘Iyāḍ de Ceuta.

  8. Tetramethyl-1-silacycloprop-2-ene: its characterization and reactions

    Energy Technology Data Exchange (ETDEWEB)

    Cornett, B.J.

    1980-05-01

    The object of this research is to fully characterize and study some of the reactions of tetramethyl-1-silacycloprop-2-ene. The final characterization of this compound was accomplished by obtaining both Si29 and 13C nuclear magnetic resonance spectra. The reactions studied included those with fluorenone, acetone, styrene, phenylacetylene, acetonitrile, t-butylcyanide, methyl isocyanate, phenyl azide, and azobenzene. Product analyses suggest that aside from addition reactions tetramethylsilacyclopropene can react either via a radical chain polymerization or an anionic chain polymerization mechanism with the latter being the more prevalent. Other reactions studied included the photolysis of tetramethylsilacyclopropene and its reactions in the presence of Pd(PPh/sub 3/)/sub 2/Cl/sub 2/. An appendix includes a study of trimethylsilyl radical disproportionation in the liquid phase to a sila olefin. Trimethylsilyl radicals in solution undergo disproportionation as well as recombination in a 1:5 ratio. The sila olefin formed by disproportionation was trapped by alcohols.

  9. Surface functionalized thiol-ene waveguides for fluorescence biosensing in microfluidic devices

    DEFF Research Database (Denmark)

    Feidenhans'l, Nikolaj Agentoft; Lafleur, Josiane P.; Jensen, Thomas Glasdam

    2013-01-01

    Thiol-ene polymers possess physical, optical, and chemical characteristics thatmake them ideal substrates for the fabrication of optofluidic devices. In this work, thiol-ene polymers are used to simultaneously create microfluidic channels and optical waveguides in one simple moulding step....... The reactive functional groups present at the surface of the thiol-ene polymer are subsequently used for the rapid, one step, site-specific functionalization of the waveguide with biological recognition molecules. It was found that while the bulk properties and chemical surface properties of thiol...... to conjugation with fluorescently labeled streptavidin. Fluorescence excitation was achieved by launching light through the thiol-ene waveguide, revealing bright fluorescent patterns along the channel/waveguide interface...

  10. Kas on plaanis tõsta lasteaia kohatasu? / Jekaterina Kuru, Rain Sangernebo, Ene Tigas ... [jt.

    Index Scriptorium Estoniae

    2011-01-01

    Küsimusele vastasid Audru lasteaia direktor Jekaterina Kuru, Nõo vallavanem Rain Sangernebo, Põlva Lepatriinu lasteaia direktor Ene Tigas, Juuru vallavalitsuse arendus- ja kultuurispetsialist Aili Normak, Alu lasteaia-algkooli direktor Ülle Riisalu

  11. Redox Mediators in Visible Light Photocatalysis: Photocatalytic Radical Thiol?Ene Additions

    OpenAIRE

    Tyson, Elizabeth L.; Niemeyer, Zachary L.; Yoon, Tehshik P.

    2014-01-01

    Synthetically useful radical thiol?ene reactions can be initiated by visible light irradiation in the presence of transition metal polypyridyl photocatalysts. The success of this method relies upon the use of p-toluidine as an essential additive. Using these conditions, high-yielding thiol?ene reactions of cysteine-containing biomolecules can be accomplished using biocompatibile wavelengths of visible light, under aqueous conditions, and with the thiol component as the limiting reagent. We pr...

  12. Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices

    DEFF Research Database (Denmark)

    Lafleur, Josiane P.; Kwapiszewski, Radoslaw; Jensen, Thomas G.

    2012-01-01

    The ability to immobilize biomolecules at specific locations on the surface of solid supports is central to many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in thiol-ene microfluidic chips. Adjusting the stoichiometric ratio of "thi...... photolithography. We also present quantitative data on the number of functional groups available for surface modification on thiol-ene substrates and their stability....

  13. Combined bead polymerization and Cinchona organocatalyst immobilization by thiol–ene addition

    Directory of Open Access Journals (Sweden)

    Kim A. Fredriksen

    2012-07-01

    Full Text Available In this work, we report an unusually concise immobilization of Cinchona organocatalysts using thiol–ene chemistry, in which catalyst immobilization and bead polymerization is combined in a single step. A solution of azo initiator, polyfunctional thiol, polyfunctional alkene and an unmodified Cinchona-derived organocatalyst in a solvent is suspended in water and copolymerized on heating by thiol–ene additions. The resultant spherical and gel-type polymer beads have been evaluated as organocatalysts in catalytic asymmetric transformations.

  14. Combined bead polymerization and Cinchona organocatalyst immobilization by thiol-ene addition.

    Science.gov (United States)

    Fredriksen, Kim A; Kristensen, Tor E; Hansen, Tore

    2012-01-01

    In this work, we report an unusually concise immobilization of Cinchona organocatalysts using thiol-ene chemistry, in which catalyst immobilization and bead polymerization is combined in a single step. A solution of azo initiator, polyfunctional thiol, polyfunctional alkene and an unmodified Cinchona-derived organocatalyst in a solvent is suspended in water and copolymerized on heating by thiol-ene additions. The resultant spherical and gel-type polymer beads have been evaluated as organocatalysts in catalytic asymmetric transformations.

  15. Draama 2007. Töörahu / Ene Paaver

    Index Scriptorium Estoniae

    Paaver, Ene, 1963-

    2008-01-01

    Ülevaade 2007. aasta eesti draamast. Pikemalt käsitletakse järgmisi autoreid ja nende näidendeid: Mart Kivastik "Tõnu võitlused", Mart Kivastik "Eesti asi", Mart Kivastik "Sõdur", Mart Kivastik "Kuninga viimane lahing ja ülikooli algus", Mart Kivastik, "Nokia 3310", Andrus Kivirähk "Voldemar", Andrus Kivirähk "Uljas neitsi", Hendrik Toompere "Kommunisti surm", Andres Ehin "Saatuslik kihlvedu ehk Volquin Varbola all", Jan Rahman "Jõujaam", Jaak Kõdar "Muinassaar", Karl Berg "Teekond valguse riiki", Angela Randmets "Lembelood vanas linnas", Toomas Kall "Tähtede sadu", Lauri Vahtre "Muna EÜE", Urmas Espenberg "Kestvad kiiduavaldused", Kalju Saaber "Koduvõõrad", Indrek Hirv "Pauluse kiriku kellad", Katrin Saukas "Võlg", Heidi Sarapuu "Austatud Riigikogu liikmed!", Heidi Sarapuu "Helisev viis", Heidi Sarapuu "Ankur hiivata", Jakob Karu "Vanaema juures", Jim Ashilevi "Nagu poisid vihma käes", Jim Ashilevi "Portselansuits", Eva Koff "Mirr", Jaak Prints "Karaoke", Kati Murutar "Mina, naine!", Ott Aardam "Börs ja Börsitar", Andrus Kivirähk, Jan Rahman, Contra "Kõrts", Mart Kase "Perekond", Villu Tamme "Haned võlgu"; Tiit Ojasoo ja Ene-Liis Semperi "GEP ehk Gorjatshije estonskije parni"

  16. Preparation and Preliminary Dielectric Characterization of Structured C60-Thiol-Ene Polymer Nanocomposites Assembled Using the Thiol-Ene Click Reaction

    Directory of Open Access Journals (Sweden)

    Hanaa M. Ahmed

    2015-11-01

    Full Text Available Fullerene-containing materials have the ability to store and release electrical energy. Therefore, fullerenes may ultimately find use in high-voltage equipment devices or as super capacitors for high electric energy storage due to this ease of manipulating their excellent dielectric properties and their high volume resistivity. A series of structured fullerene (C60 polymer nanocomposites were assembled using the thiol-ene click reaction, between alkyl thiols and allyl functionalized C60 derivatives. The resulting high-density C60-urethane-thiol-ene (C60-Thiol-Ene networks possessed excellent mechanical properties. These novel networks were characterized using standard techniques, including infrared spectroscopy (FTIR, differential scanning calorimetry (DSC, dynamic mechanical analysis (DMA, and thermal gravimetric analysis (TGA. The dielectric spectra for the prepared samples were determined over a broad frequency range at room temperature using a broadband dielectric spectrometer and a semiconductor characterization system. The changes in thermo-mechanical and electrical properties of these novel fullerene-thiol-ene composite films were measured as a function of the C60 content, and samples characterized by high dielectric permittivity and low dielectric loss were produced. In this process, variations in chemical composition of the networks were correlated to performance characteristics.

  17. Ene-Liis Semper : hindan päeva, kui teen ühte asja / Ene-Liis Semper ; interv. Jaanus Kulli

    Index Scriptorium Estoniae

    Semper, Ene-Liis, 1969-

    2008-01-01

    Teatri- ja videokunstnik Ene-Liis Semper oma elust ja tööst. Lisaks repliik "Parim päev on tööst prii päev" ja peagi Teater NO99s esietenduvast William Shakespeare'i lavastusest "Muinasjutuline "Perikles"

  18. Simple preparation of thiol-ene particles in glycerol and surface functionalization by thiol-ene chemistry (TEC) and surface chain transfer free radical polymerization (SCT-FRP)

    DEFF Research Database (Denmark)

    Hoffmann, Christian; Chiaula, Valeria; Pinelo, Manuel

    2017-01-01

    Thiol-ene (TE) based polymer particles have traditionally been prepared via emulsion polymerization in water (using surfactants, stabilizers and co-solvents). Here, we present a green and simple alternative with excellent control over particle size, while avoiding the addition of stabilizers...

  19. Simple preparation of thiol-ene particles in glycerol and surface functionalization by thiol-ene chemistry (TEC) and surface chain transfer free radical polymerization (SCT-FRP)

    DEFF Research Database (Denmark)

    Hoffmann, Christian; Chiaula, Valeria; Pinelo, Manuel

    2017-01-01

    functionalization of excess thiol groups via photochemical thiol-ene chemistry (TEC) resulting in a functional monolayer. In addition, surface chain transfer free radical polymerization (SCT-FRP) was used for the first time to introduce a thicker polymer layer on the particle surface. The application potential...

  20. Functionalization of embedded thiol-ene waveguides for evanescent wave induced fluorescence detection in a microfluidic device

    DEFF Research Database (Denmark)

    Feidenhans'l, Nikolaj Agentoft; Jensen, Thomas Glasdam; Lafleur, Josiane P.

    2013-01-01

    We demonstrate the use of functional surface groups inherently present on off-stoichiometric thiol−ene polymers, for site-specific immobilization of biomolecules and detection by evanescent wave-induced fluorescence. An optofluidic chip featuring an embedded thiol−ene waveguide was selectively...... functionalized with biotin using photografting. The biotin was used for immobilization of fluorescently labelled streptavidin, and experiments revealed a linear correlation between streptavidin concentration and fluorescent intensity. To further demonstrate the attractiveness of using thiol−ene for optofluidic...... devices, the optical properties of thiol−ene was evaluated by determining the transparency and refractive index of the cured polymer....

  1. Functionalization of embedded thiol-ene waveguides for evanescent wave-induced fluorescence detection in a microfluidic device

    DEFF Research Database (Denmark)

    Feidenhans, Nikolaj A.; Jensen, Thomas Glasdam; Lafleur, Josiane P.

    2013-01-01

    We demonstrate the use of functional surface groups inherently present on off-stoichiometric thiol-ene polymers, for site-specific immobilization of biomolecules and detection by evanescent wave-induced fluorescence. An optofluidic chip featuring an embedded thiol-ene waveguide was selectively...... functionalized with biotin using photografting. The biotin was used for immobilization of fluorescently labelled streptavidin, and experiments revealed a linear correlation between streptavidin concentration and fluorescent intensity. To further demonstrate the attractiveness of using thiol-ene for optofluidic...... devices, the optical properties of thiol-ene was evaluated by determining the transparency and refractive index of the cured polymer....

  2. Kujundades ja tootes / Mart Kalm

    Index Scriptorium Estoniae

    Kalm, Mart, 1961-

    2010-01-01

    Uurimata disain. Tsaariaegse tööstuse eripära. Lutheri vabrik. Teised mööblivalmistajad. Ilmus sisearhitekt. Kujundatud ühiskauplus. Uued tootmisharud. Kunsttööstuskooli panus. Nahkehistöö. Langebrauni kirjatud nõud. Lorupi klaas ja kristall. Kopfi ja Tavasti metallitööd. Naiste näputööd

  3. Vabalt kulgenud arhitektuuritriennaal / Mart Kalm

    Index Scriptorium Estoniae

    Kalm, Mart, 1961-

    1999-01-01

    IV Põhja- ja Baltimaade arhitektuuritriennaal "Vaba kulg. Free Way" Rotermanni soolalaos. Hani Rashidi, rühmituse Ocean, Jan Olov Jenseni, Urmas Muru, Gert B. Wingõrdhi, Frei Otto, Ilja Lezhava, Tapio Periäineni ettekannetest.

  4. Surface modification of cyclomatrix polyphosphazene microsphere by thiol-ene chemistry and lectin recognition

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Chen; Zhu, Xue-yan; Gao, Qiao-ling; Fang, Fei; Huang, Xiao-jun, E-mail: hxjzxh@zju.edu.cn

    2016-11-30

    Graphical abstract: A new synthetic route leading to polyphosphazene cyclomatrix microsphere with various functional groups has achieved via thiol-ene click modification. Herein, hexacholorocyclophosphazene (HCCP) crosslinked with bisphenol-S and 4,4′-diallyl bisphenol-S to generate broadly dispersed microspheres. Thiol-ene modification under UV irradiation not only presented high efficiency and flexibility for post-functionalization, but also imposed no harm on global morphology and crosslinked skeleton of such microspheres. - Highlights: • Functional polyphosphazene microspheres with high chemical flexibility were synthesized by thiol-ene modification. • Polyphosphazene microspheres possessed high thermal stability. • Glycosylated polyphosphazene microspheres showed affinity to lectin Con-A, which inferred potential application in biomedicine. - Abstract: A new synthetic route leading to functional polyphosphazene cyclomatrix microsphere has been developed via thiol-ene click modification. Hexacholorocyclophosphazene (HCCP) was crosslinked with both bisphenol-S and 4,4′-diallyl bisphenol-S to obtain vinyl polyphosphazene microspheres (VPZM) in order to ensure high crosslinking degree and introduce vinyl moieties. Compared to the microspheres obtained by HCCP and bisphenol-S, the size of VPZM was broadly dispersed from 400 nm to 1.40 μm. Thiol-ene click reactions were carried out to attach functional groups, such as glucosyl, carboxyl, ester and dodecyl groups onto polyphosphazene microspheres, which demonstrated no change in morphology and size after modification. Solid state NMR (SSNMR) and Fourier transform infrared spectoscopy (FT-IR) results showed that the vinyl moieties were introduced in the period of crosslinking and functionalization was also successful via click reactions. Moreover, the microspheres presented a little difference in thermal properties after modification. Concanavalin A (Con-A) fluorescent adsorption was also observed for

  5. Thiol-ene functionalized siloxanes for use as elastomeric dental impression materials.

    Science.gov (United States)

    Cole, Megan A; Jankousky, Katherine C; Bowman, Christopher N

    2014-04-01

    Thiol- and allyl-functionalized siloxane oligomers are synthesized and evaluated for use as a radical-mediated, rapid set elastomeric dental impression material. Thiol-ene siloxane formulations are crosslinked using a redox-initiated polymerization scheme, and the mechanical properties of the thiol-ene network are manipulated through the incorporation of varying degrees of plasticizer and kaolin filler. Formulations with medium and light body consistencies are further evaluated for their ability to accurately replicate features on both the gross and microscopic levels. We hypothesize that thiol-ene functionalized siloxane systems will exhibit faster setting times and greater detail reproduction than commercially available polyvinylsiloxane (PVS) materials of comparable consistencies. Thiol-ene functionalized siloxane mixtures formulated with varying levels of redox initiators, plasticizer, and kaolin filler are made and evaluated for their polymerization speed (FTIR), consistency (ISO4823.9.2), and surface energy (goniometer). Feature replication is evaluated quantitatively by SEM. The Tg, storage modulus, and creep behavior are determined by DMA. Increasing redox initiation rate increases the polymerization rate but at high levels also limits working time. Combining 0.86 wt% oxidizing agent with up to 5 wt% plasticizer gave a working time of 3 min and a setting time of 2 min. The selected medium and light body thiol-ene formulations also achieved greater qualitative detail reproduction than the commercial material and reproduced micrometer patterns with 98% accuracy. Improving detail reproduction and setting speed is a primary focus of dental impression material design and synthesis. Radical-mediated polymerizations, particularly thiol-ene reactions, are recognized for their speed, reduced shrinkage, and 'click' nature. Copyright © 2014 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

  6. Rapid and simple preparation of thiol-ene emulsion-templated monoliths and their application as enzymatic microreactors

    DEFF Research Database (Denmark)

    Lafleur, Josiane P; Senkbeil, Silja; Novotny, Jakub

    2015-01-01

    electron microscopy showed that the methanol-based emulsion templating process resulted in a network of highly interconnected and regular thiol-ene beads anchored solidly inside thiol-ene microchannels. Surface area measurements indicate that the monoliths are macroporous, with no or little micro...

  7. Crosslinking and degradation of step-growth hydrogels formed by thiol-ene photo-click chemistry

    Science.gov (United States)

    Shih, Han; Lin, Chien-Chi

    2014-01-01

    Thiol-ene photo-click hydrogels have been used for a variety of tissue engineering and controlled release applications. In this step-growth photopolymerization scheme, multi-arm poly(ethylene glycol) norbornene (PEG4NB) was crosslinked with di-thiol containing crosslinkers to form chemically crosslinked hydrogels. While the mechanism of thiol-ene gelation was well described in the literature, its network ideality and degradation behaviors are not well-characterized. Here, we compared the network crosslinking of thiol-ene hydrogels to Michael-type addition hydrogels and found thiol-ene hydrogels formed with faster gel points and higher degree of crosslinking. However, thiol-ene hydrogels still contained significant network non-ideality, demonstrated by a high dependency of hydrogel swelling on macromer contents. In addition, the presence of ester bonds within the PEG-norbornene macromer rendered thiol-ene hydrogels hydrolytically degradable. Through validating model predictions with experimental results, we found that the hydrolytic degradation of thiol-ene hydrogels was not only governed by ester bond hydrolysis, but also affected by the degree of network crosslinking. In an attempt to manipulate network crosslinking and degradation of thiol-ene hydrogels, we incorporated peptide crosslinkers with different sequences and characterized the hydrolytic degradation of these PEG-peptide hydrogels. In addition, we incorporated a chymotrypsin-sensitive peptide as part of the crosslinkers to tune the mode of gel degradation from bulk degradation to surface erosion. PMID:22708824

  8. 3-(1H-Indol-3-yl-4-(morpholin-4-ylcyclobut-3-ene-1,2-dione

    Directory of Open Access Journals (Sweden)

    Johann Grünefeld

    2012-06-01

    Full Text Available 3-(1H-Indol-3-yl-4-(morpholin-4-ylcyclobut-3-ene-1,2-dione was obtained in good yields (72–82% by nucleophilic substitution of 3-chloro-4-(1H-indol-3-ylcyclobut-3-ene-1,2-dione with morpholine.

  9. Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices

    DEFF Research Database (Denmark)

    Lafleur, Josiane P.; Kwapiszewski, Radoslaw; Jensen, Thomas Glasdam

    2013-01-01

    The suitable optical properties of thiol–ene polymers combined with the ease of modifying their surface for the attachment of recognition molecules make them ideal candidates in many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules...... ! 17 SH nm"2. Biotin alkyne was patterned directly inside thiol–ene microchannels prior to conjugation with fluorescently labelled streptavidin. The surface bound conjugates were detected by evanescent waveinduced fluorescence (EWIF), demonstrating the success of the grafting procedure and its...... potential for biochip applications....

  10. Preparation and Characterization of Fluorinated Hydrophobic UV-Crosslinkable Thiol-Ene Polyurethane Coatings

    Directory of Open Access Journals (Sweden)

    Wenjing Xia

    2017-08-01

    Full Text Available The polyurethane prepolymer terminated with a double bond was synthesized using isophorone diisocyanate (IPDI, hydroxyl terminated polybutadiene (HTPB, 1,4-butanediol (BDO, and 2-hydroxyethyl acrylate (HEA. Then, a series of innovative UV-curable polyurethane coatings were prepared by blending ene-terminated polyurethane, fluoroacrylate monomer, and multifunctional thiol crosslinker upon UV exposure. The incorporation of fluoroacrylate monomer and multifunctional thiols into polyurethane coatings significantly enhanced the hydrophobic property, mechanical property, pencil hardness, and glossiness of the polyurethane coatings. This method of preparing UV crosslinkable, hydrophobic polyurethane coatings based on thiol-ene chemistry exhibited numerous advantages over other UV photocuring systems.

  11. Mait Agu nimelise stipendiumi pälvivad aastal 2013 Eliisa Sokk ja Ene Jakobson

    Index Scriptorium Estoniae

    2013-01-01

    Tallinna Ülikooli koreograafia osakond annab Mait Agu nimelist stipendiumi välja viieteistkümnendat korda. Stipendiumi saavad TLÜ koreograafia osakonna 3. kursuse tudeng Eliisa Sokk ja TLÜ koreograafia eriala 2. lennu lõpetaja, tantsuansambli "Sõleke" looja ning juht Ene Jakobson

  12. Preparation of Biomolecule Microstructures and Microarrays by Thiol–ene Photoimmobilization

    NARCIS (Netherlands)

    Weinrich, Dirk; Köhn, Maja; Jonkheijm, Pascal; Westerlind, Ulrika; Dehmelt, Leif; Engelkamp, Hans; Christianen, Peter C.M.; Kuhlmann, Jürgen; Maan, Jan C.; Nüsse, Dirk; Schröder, Hendrik; Wacker, Ron; Voges, Edgar; Breinbauer, Rolf; Kunz, Horst; Niemeyer, Christof M.; Waldmann, Herbert

    2010-01-01

    A mild, fast and flexible method for photoimmobilization of biomolecules based on the light-initiated thiol–ene reaction has been developed. After investigation and optimization of various surface materials, surface chemistries and reaction parameters, microstructures and microarrays of biotin,

  13. Patterned Hydrophilization of Nanoporous 1,2‐PB by Thiol‐ene Photochemistry

    DEFF Research Database (Denmark)

    Berthold, Anton; Sagar, Kaushal Shashikant; Ndoni, Sokol

    2011-01-01

    is monitored by FT‐IR, UV–Vis, contact angle, and gravimetry. Overall quantum yields are calculated for the two thiol‐ene “click” reactions in nano‐confinement, neatly revealing their chain‐like nature. Top–down photolithographic patterning is demonstrated, realizing hydrophilic nanoporous “corridors...

  14. On-resin peptide macrocyclization using thiol-ene click chemistry

    OpenAIRE

    Aimetti, Alex A.; Shoemaker, Richard K.; Lin, Chien-Chi; Anseth, Kristi S

    2010-01-01

    A versatile and rapid synthetic strategy has been developed for the on-resin cyclization of peptides using thiol-ene photochemistry. This unique method exploits the thiol group of natural cysteine amino acids and allows for various alkenes to be incorporated orthogonal to the peptide backbone.

  15. Efforts toward rapid construction of the cortistatin A carbocyclic core via enyne-ene metathesis

    KAUST Repository

    Baumgartner, Corinne

    2010-01-01

    Our efforts toward the construction of the carbocylic core of cortistatin A via an enyne-ene metathesis are disclosed. Interestingly, an attempted S N2 inversion of a secondary mesylate in our five-membered D-ring piece gave a product with retention of stereochemistry. © 2010 The Royal Society of Chemistry.

  16. Functional monolayers on oxide-free silicon surfaces via thiol–ene click chemistry

    NARCIS (Netherlands)

    Caipa Campos, Mabel A.; Paulusse, Jos Marie Johannes; Zuilhof, Han

    2010-01-01

    Thiol–ene click chemistry was used for the attachment of a variety of functional molecules onto oxide-free Si(111) surfaces using very mild conditions; the efficient nature of this coupling strategy allowed for successful light-induced micropatterning and thus provides a novel route towards

  17. Development of Thermal and Photochemical Strategies for Thiol−Ene Click Polymer Functionalization

    NARCIS (Netherlands)

    Campos, Luis M.; Killops, Kato L.; Sakai, Ryosuke; Paulusse, Jos Marie Johannes; Damiron, Denis; Drockenmuller, Eric; Messmore, Benjamin W.; Hawker, Craig J.

    2008-01-01

    A series of alkene-functional polymers were synthesized by controlled polymerization techniques in order to investigate and compare the efficiency and orthogonality of both photochemically and thermally initiated thiol−ene click coupling reactions. The copolymers were designed to have single or

  18. DFT Investigation of the Palladium-Catalyzed Ene-Yne Coupling

    DEFF Research Database (Denmark)

    Henriksen, Signe Teuber; Tanner, David Ackland; Skrydstrup, T.

    2010-01-01

    ). Concerning chemoselectivity, the calculations also clarify why the ene-yne coupling is able to dominate over plausible alternative reaction pathways such as alkene homocoupiing and alkyne polymerization. The role of the phosphine ligand at various stages of the catalytic cycle has also been delineated....

  19. Electron swarm parameter measurements of perfluorobut-2-ene (2-C4F8)

    OpenAIRE

    Chachereau, Alise; Franck, Christian M.

    2015-01-01

    In this contribution, the electron swarm parameters of perfluorobut-2-ene (2-C4F8) mixtures with the buffer gases N2, CO2 and Argon are experimentally investigated. The effective ionization rate constant and electron drift velocity are measured in the entire E/N range up to the critical density-reduced electric field.

  20. Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices

    DEFF Research Database (Denmark)

    Lafleur, Josiane P.; Kwapiszewski, Radoslaw; Jensen, Thomas Glasdam

    2012-01-01

    ” and “ene” monomers present in the microfluidic chip bulk material provides a simple and efficient way of tuning the chip’s surface chemistry. Here, thiol-ene chips displaying an excess of functional thiol groups at their surfaces are functionalized with biotin and streptavidin in a controlled fashion using...

  1. A Study of Functional Polymer Colloids Prepared Using Thiol-Ene/Yne Click Chemistry

    Science.gov (United States)

    Durham, Olivia Z.

    This project demonstrates the first instance of thiol-ene chemistry as the polymerization method for the production of polymer colloids in two-phase heterogeneous suspensions, miniemulsions, and emulsions. This work was also expanded to thiol-yne chemistry for the production of polymer particles containing increased crosslinking density. The utility of thiol-ene and thiol-yne chemistries for polymerization and polymer modification is well established in bulk systems. These reactions are considered 'click' reactions, which can be defined as processes that are both facile and simple, offering high yields with nearly 100% conversion, no side products, easy product separation, compatibility with a diverse variety of commercially available starting materials, and orthogonality with other chemistries. In addition, thiol-ene and thiol-yne chemistry follow a step-growth mechanism for the development of highly uniform polymer networks, where polymer growth is dependent on the coupling of functional groups. These step-growth polymerization systems are in stark contrast to the chain-growth mechanisms of acrylic and styrenic monomers that have dominated the field of conventional heterogeneous polymerizations. Preliminary studies evaluated the mechanism of particle production in suspension and miniemulsion systems. Monomer droplets were compared to the final polymer particles to confirm that particle growth occurred through the polymerization of monomer droplets. Additional parameters examined include homogenization energy (mechanical mixing), diluent species and concentration, and monomer content. These reactions were conducted using photoinitiation to yield particles in a matter of minutes with diameters in the size range of several microns to hundreds of microns in suspensions or submicron particles in miniemulsions. Improved control over the particle size and size distribution was examined through variation of reaction parameters. In addition, a method of seeded suspension

  2. Development of a thiol-ene based screening platform for enzyme immobilization demonstrated using horseradish peroxidase

    DEFF Research Database (Denmark)

    Hoffmann, Christian; Pinelo, Manuel; Woodley, John

    2017-01-01

    functionalization by thiol-ene chemistry (TEC) resulted in the formation of a functional monolayer in each well, whereas, polymer surface grafts were introduced through surface chain transfer free radical polymerization (SCT-FRP). Enzyme immobilization on the modified surfaces was evaluated by using a rhodamine...... labeled horseradish peroxidase (Rho-HRP) as a model enzyme, and the amount of immobilized enzyme was qualitatively assessed by fluorescence intensity (FI) measurements. Subsequently, Rho-HRP activity was measured directly on the surface. The broad range of utilized surface chemistries permits direct...... correlation of enzymatic activity to the surface functionality and improves the determination of promising enzyme-surface candidates. The results underline the high potential of this system as a screening platform for synergistic immobilization of enzymes onto thiol-ene polymer surfaces. This article...

  3. Hybrid thiol-ene network nanocomposites based on multi(meth)acrylate POSS.

    Science.gov (United States)

    Li, Liguo; Liang, Rendong; Li, Yajie; Liu, Hongzhi; Feng, Shengyu

    2013-09-15

    First, multi(meth)acrylate functionalized POSS monomers were synthesized in this paper. Secondly, FTIR was used to evaluate the homopolymerization behaviors of multi(meth)acrylate POSS and their copolymerization behaviors in the thiol-ene reactions with octa(3-mercaptopropyl) POSS in the presence of photoinitiator. Results showed that the photopolymerization rate of multimethacrylate POSS was faster than that of multiacrylate POSS. The FTIR results also showed that the copolymerizations were dominant in the thiol-ene reactions with octa(3-mercaptopropyl) POSS, different from traditional (meth)acrylate-thiol system, in which homopolymerizations were predominant. Finally, the resulted hybrid networks based on POSS were characterized by XRD, FE-SEM, DSC, and TGA. The characterization results showed that hybrid networks based on POSS were homogeneous and exhibited high thermal stability. Copyright © 2013 Elsevier Inc. All rights reserved.

  4. Acyloxylation of 1-methoxycyclohex-1-ene and other enol ethers with dimethyl peroxydicarbonate

    OpenAIRE

    Schank, Kurt; Beck, Horst; Pistorius, Susanne; Rapold, Thomas

    1995-01-01

    Acyloxylation of different types of enol ethers (derived from aldehydes and ketones) by dimethyl peroxydicarbonate (DPDC) results in either addition to the double bond or in a formal replacement of an allylic hydrogen by a methoxycarbonyloxy group forming vicinal oxygenated hydrocarbons. 1,3-oxygenated products via monoacyloxylation could not be observed. The results with 1-methoxycyclohex-1-ene (1a) are compared with copper(I)-salt catalyzed acyloxylations by means of tert. butyl peroxycarbo...

  5. Heterofunctional Glycopolypeptides by Combination of Thiol-Ene Chemistry and NCA Polymerization.

    Science.gov (United States)

    Krannig, Kai-Steffen; Schlaad, Helmut

    2016-01-01

    Glycopolypeptides are prepared either by the polymerization of glycosylated amino acid N-carboxyanhydrides (NCAs) or by the post-polymerization functionalization of polypeptides with suitable functional groups. Here we present a method for the in-situ functionalization and (co-) polymerization of allylglycine N-carboxyanhydride in a facile one-pot procedure, combining radical thiol-ene photochemistry and nucleophilic ring-opening polymerization techniques, to yield well-defined heterofunctional glycopolypeptides.

  6. Ene-Liis Semper : Kohalolu piiritsoonis = Presence in the border zone / Anu Allas

    Index Scriptorium Estoniae

    Allas, Anu, 1977-

    2007-01-01

    Teatri- ja videokunstnik Ene-Liis Semperi peamiselt 1990-ndate aastate videotest, mis on valdavalt rõhutatult lavastuslikud, paisutatult sümbolistlikud või kus on esitatud piirsituatsioone, ahistust ning selle teadlikku eksponeerimist väljakutsena publikule. Iseennast paljastades, alandades ja ohtu seades jääb kunstnik enamasti jahedalt distantseerituks, publikule jääb aga arusaamatuks, kas tegemist on ohvri, kangelanna või osava mänguriga

  7. One-pot, additive-free preparation of functionalized polyurethanes via amine-thiol-ene conjugation

    OpenAIRE

    Espeel, Pieter; Goethals, Fabienne; Driessen, Frank; Nguyen, Le Thu Thi; Du Prez, Filip

    2013-01-01

    A straightforward, isocyanate-free method for the synthesis of functionalized polyurethanes, based on amine-thiol-ene conjugation, was elaborated. Aminolysis of a readily available AB'-urethane monomer, containing both an acrylate (A) and a thiolactone unit (B'), facilitates the preparation of various reactive thiol-acrylates. In situ polymerization via Michael addition proceeds under ambient conditions, yielding polyurethanes with a large variety of chemical functionalities. Side-chain funct...

  8. Thiol-Ene Based Polymer Waveguides Fabricated By Uv-Assisted Soft Lithography For Optofluidic Applications

    DEFF Research Database (Denmark)

    Zhuang, Guisheng; Jensen, Thomas Glasdam; Kutter, Jörg Peter

    2011-01-01

    In this paper, a thiol-ene based polymer waveguide, defined by UV-assisted soft lithography, is designed, fabricated and characterized. Waveguides are formed by filling microfluidic channels with a high refractive index liquid mixture of ‘thiol’ and ‘ene’ monomers (e.g., trimethylolpropane tris(3...... thiol-ene based polymers as waveguide core materials for potential optofluidic applications.......In this paper, a thiol-ene based polymer waveguide, defined by UV-assisted soft lithography, is designed, fabricated and characterized. Waveguides are formed by filling microfluidic channels with a high refractive index liquid mixture of ‘thiol’ and ‘ene’ monomers (e.g., trimethylolpropane tris(3......-mercaptopropionate) = ‘thiol’, and 1,3,5-triallyl-1,3,5-triazine-2,4,6(1H,3H,5H)-trione = ‘ene’), which can be cured by UV exposure into a solid polymer. The waveguides demonstrated good confinement of light, and a propagation loss of 0.5 dB/cm was obtained. To our best knowledge, this is the first report to employ...

  9. Facile and Efficient Synthesis of Carbosiloxane Dendrimers via Orthogonal Click Chemistry Between Thiol and Ene.

    Science.gov (United States)

    Zhang, Zhida; Feng, Shengyu; Zhang, Jie

    2016-02-01

    A combination of a thiol-Michael addition reaction and a free radical mediated thiol-ene reaction is employed as a facile and efficient approach to carbosiloxane dendrimer synthesis. For the first time, carbosiloxane dendrimers are constructed rapidly by an orthogonal click strategy without protection/deprotection procedures. The chemoselectivity of these two thiol-ene click reactions leads to a design of a new monomer containing both electron-deficient carbon-carbon double bonds and unconjugated carbon-carbon double bonds. Siloxane bonds are introduced as the linker between these two kinds of carbon-carbon double bonds. Starting from a bifunctional thiol core, the dendrimers are constructed by iterative thiol-ene click reactions under different but both mild reaction conditions. After simple purification steps the fifth dendrimer with 54 peripheral functional groups is obtained with an excellent overall yield in a single day. Furthermore, a strong blue glow is observed when the dendrimer is excited by a UV lamp. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  10. An orthogonal, one-pot, simultaneous UV-mediated route to thiol-ene/sol-gel film

    Directory of Open Access Journals (Sweden)

    A. Chemtob

    2016-06-01

    Full Text Available We describe a novel combination of orthogonal reactions based on UV-driven thiol–ene and alkoxysilyl condensation reactions to form a single-step route toward thioether-bridged silsesquioxane films. Our chemical strategy consists of using two bifunctional (methacrylate (E and propanethiol (T trimethoxysilyl precursors containing two complementary functional moieties for thiol-ene coupling and sol-gel process. The reaction kinetics revealed that c.a. 85% of thiol and ene conversions were consumed concomitantly. Meanwhile, a complete hydrolysis was accomplished, affording ultimately a high degree of condensation (81%. Emphasis was placed on differences of mechanical properties between sol-gel hybrids resulting from thiol-ene reaction (E-T mixture and ene homopolymerization (E only using scratch test measurements. For the methacrylate system, the formation of thioether linkages within a vitreous silica network emerged as a useful strategy for the formation of a uniform, low-stress and flexible crosslinked hybrid structure. Enhanced mechanical properties were manifested by an expanded elastic domain, and better resistance to cracking. Moreover, there are clear indications that mechanical properties can be easily tuned upon varying the ratio of the two hybrid precursors.

  11. Advantages and drawbacks of Thiol-ene based resins for 3D-printing

    Science.gov (United States)

    Leonards, Holger; Engelhardt, Sascha; Hoffmann, Andreas; Pongratz, Ludwig; Schriever, Sascha; Bläsius, Jana; Wehner, Martin; Gillner, Arnold

    2015-03-01

    The technology of 3D printing is conquering the world and awakens the interest of many users in the most varying of applications. New formulation approaches for photo-sensitive thiol-ene resins in combination with various printing technologies, like stereolithography (SLA), projection based printing/digital light processing (DLP) or two-photon polymerization (TPP) are presented. Thiol-ene polymerizations are known for its fast and quantitative reaction and to form highly homogeneous polymer networks. As the resins are locally and temporally photo-curable the polymerization type is very promising for 3D-printing. By using suitable wavelengths, photoinitiator-free fabrication is feasible for single- and two photon induced polymerization. In this paper divinyl ethers of polyethylene glycols in combination with star-shaped tetrathiols were used to design a simple test-system for photo-curable thiol-ene resins. In order to control and improve curing depth and lateral resolution in 3D-polymerization processes, either additives in chemical formulation or process parameters can be changed. The achieved curing depth and resolution limits depend on the applied fabrication method. While two-/multiphoton induced lithography offers the possibility of micron- to sub-micron resolution it lacks in built-up speed. Hence single-photon polymerization is a fast alternative with optimization potential in sub-10-micron resolution. Absorber- and initiator free compositions were developed in order to avoid aging, yellowing and toxicity of resulting products. They can be cured with UV-laser radiation below 300 nm. The development at Fraunhofer ILT is focusing on new applications in the field of medical products and implants, technical products with respect to mechanical properties or optical properties of 3D-printed objects. Recent process results with model system (polyethylene glycol divinylether/ Pentaerithrytol tetrakis (3-mercaptopropionat), Raman measurements of polymer conversion

  12. Roll-to-plate fabrication of microfluidic devices with rheology-modified thiol-ene resins

    DEFF Research Database (Denmark)

    Senkbeil, Silja; Aho, Johanna; Yde, Leif

    2016-01-01

    In this paper, the replication possibilities of microfluidic channels by UV-roll-to-plate fabrication were investigated and a study of rheology-modified thiol-ene for the application in such a UV-roll-to-plate setup was conducted. The system allows the manufacture of channels with aspect ratios...... microfluidic channels with 40 × 95 μm cross-sectional dimensions. Moreover, deeper (90 μm) channels can be fabricated with highly viscous resins based on thiol-terminated oligomers. As a demonstration, capillary electrophoresis chips were prepared and tested for a simple separation of two fluorescent dyes....

  13. Synthesis of 2-ene-1,4-diols by a new cascade-opening of 1,3-diepoxides: towards an efficient synthesis of dihydroxytaxoid derivatives.

    Science.gov (United States)

    Aldegunde, María J; Castedo, Luis; Granja, Juan R

    2009-01-01

    Taxing taxanes: A new method for the preparation of alk-2-ene-1,4-diols from 1,3-dienes, especially for geometrical constrained derivatives, via dioxirane derivative formation followed by Ti(III)-induced oxirane ring opening, is described. The strategy has been very efficiently implemented to the construction of the 11-ene-10,13-diol moiety present in taxol derivatives.

  14. Use of a continuous-flow microreactor for thiol-ene functionalization of RAFT-derived poly(butyl acrylate)

    OpenAIRE

    Vandenbergh, Joke; Junkers, Thomas

    2012-01-01

    This study describes the synthesis of functionalized RAFT-derived poly(n-butyl acrylate) polymers via the use of a continuous-flow microreactor, in which aminolysis as well as thiol-ene reactions are executed in reaction times of just 20 minutes. Poly(n-butyl acrylate) (M-n = 3800 g mol(-1), PDI = 1.10) with a trithiocarbonate end group was prepared via a conventional RAFT process. The polymer was then functionalized via aminolysis/thiol-ene reactions in the micro-flow reactor with isobornyl ...

  15. Synthesis and characterization of thiol-ene functionalized siloxanes and evaluation of their polymerization kinetics, network properties, and dental applications

    Science.gov (United States)

    Cole, Megan A.

    We explored formation-structure-property relationships in thiol-ene functionalized oligosiloxanes to create crosslinked networks. Specifically, nine oligomers were synthesized, three with thiol-functional silane repeats and three with allyl-functional silane repeats. Structural variations in each oligomer were systematically induced through the incorporation of non-reactive repeats bearing either diphenyl or di-n-octyl moieties, and the oligomer molecular weight was limited by the presence of monofunctional silane condensation species. The molecular weights and chain compositions of all oligomers were ascertained and subsequently used in the evaluation of network properties formed upon photopolymerization of thiol- and ene-functional reactants. Polymerization kinetics of the thiol-ene functionalized siloxanes were also investigated using photoinitiation owing to the spatial and temporal control afforded by this technique. In particular, the effects of the viscosity of the ene-functionalized oligomer and the degree of thiol functionalization on the observed polymerization rate were determined. Results showed that the speed of polymerization varied with changes to the rate-limiting step, which was heavily influenced by neighboring non-reactive functionalities. Moreover, the thiol-ene reaction was found to exhibity unimolecular termination exclusively in siloxane-based systems. Proposed use of the thiol-ene functionalized siloxane system as a dental impression material necessitated the development of a redox initiation scheme. Evaluation of the benzoylperoxide/dimethyl-p-toluidine redox pair in traditional systems showed bulk thiol-ene polymerizations comparable to photoinitiation with the added advantage of uninhibited depth control, as also demonstrated in small molecule thiol-ene coupling reactions initiated by this same redox system. Application of the redox pair to the siloxane system allowed for the viscoelastic properties as well as the feature replication

  16. Advanced functionalization of polyhydroxyalkanoate via the UV-initiated thiol-ene click reaction.

    Science.gov (United States)

    Tajima, Kenji; Iwamoto, Kosuke; Satoh, Yasuharu; Sakai, Ryosuke; Satoh, Toshifumi; Dairi, Tohru

    2016-05-01

    Polyhydroxyalkanoates (PHAs) incorporating vinyl-bearing 3-hydroxyalkanoates were prepared in 8.5-12.9 g L(-1) yield. The molar ratios (0-16 mol%) of the vinyl-bearing 3-hydroxyalkanoate derivatives were controlled by the continuous feeding of undecylenate at various concentrations. Subsequently, the PHAs were functionalized by UV-initiated thiol-ene click reaction and chemical modification. (1)H NMR spectra suggested that 3-mercaptopropionic acid and 2-aminoethanethiol were successfully introduced into the vinyl-bearing PHA. Subsequently, chemical modification using fluorescein or a fibronectin active fragment (GRGDS) was attempted. The former yielded a PHA derivative capable of emitting fluorescence under UV irradiation, which was useful for determining the miscibility of PHA in a composite film comprising poly-ʟ-lactic acid (PLLA) and PHA. In the latter case, PHA bearing GRGDS peptides exhibited cell adhesiveness, suggesting that its biocompatibility was improved upon peptide introduction. Taken together, the UV-initiated thiol-ene click reaction was demonstrated to be useful in PHA modification.

  17. Self-Healing Photocurable Epoxy/thiol-ene Systems Using an Aromatic Epoxy Resin

    Directory of Open Access Journals (Sweden)

    Ricardo Acosta Ortiz

    2016-01-01

    Full Text Available A rapid and efficient method to obtain self-healing epoxy resins is discussed. This method is based on the use of a thiol-disulfide oligomer obtained by partial oxidation of a multifunctional thiol using a hypervalent iodine (III compound as oxidant. The oligomer was characterized by Fourier transform infrared spectroscopy (FTIR, Raman and nuclear magnetic resonance spectroscopies, and gel permeation chromatography (GPC. The oligomer was a joint component of the thiol-ene system along with a tetra-allyl-functionalized curing agent. The kinetics of the photopolymerization of diglycidylether of bisphenol A (DGEBA revealed that conversions of the epoxy groups as high as 80% were achieved in only 15 minutes by increasing the concentration of the thiol-ene system in the formulation. The disulfide bonds introduced in the copolymer using the thiol-disulfide oligomer allowed the repairing of the test specimens in as little as 10 minutes when the specimens were heated at 80°C or for 500 minutes at room temperature. The analysis of the mechanical properties using dynamic mechanical analysis (DMA showed that the specimens displayed a healing efficiency up to 111% compared with the unhealed specimens, depending on the amount of polythioethers present in the copolymer.

  18. Kas erivajadustega lapsed saavad õigel ajal abi? / Ene Mägi, Urve Raudsepp-Alt, Ale Sprenk, Peeter Aas

    Index Scriptorium Estoniae

    2009-01-01

    Küsimusele vastavad: Tallinna Ülikooli Kasvatusteaduste Instituudi eri- ja sotsiaalpedagoogika osakonna juhataja Ene Mägi, Tallinna Haridusameti üldhariduse osakonna peaspetsialist Urve Raudsepp-Alt, Krabi põhikooli direktor Ale Sprenk, Põlva Maavalitsuse haridus-, kultuuri- ja sotsiaalosakonna juhataja Peeter Aas

  19. Mida teie oma tervise heaks teete? / Ly Jagor, Ülle Mihhailova, Ene Pill, Katrin Käbin...[jt.

    Index Scriptorium Estoniae

    2008-01-01

    Küsimusele vastavad Pärnu Õppenõustamiskeskuse psühholoog Ly Jagor, Maasika lasteaia juhataja asetäitja Ülle Mihhailova, Tallinna Perekeskuse ja Tähetorni lastekeskuse psühholoog Ene Pill, Tallinna Nõmme Noortemaja väikelaste ringijuht Katrin Käbin, Tääksi Põhikooli õpetaja Silva Kolk

  20. Photoinduced thiol-ene polymerization reaction for fast preparation of macroporous hybrid monoliths and their application in capillary liquid chromatography.

    Science.gov (United States)

    Liu, Zhongshan; Ou, Junjie; Lin, Hui; Liu, Zheyi; Wang, Hongwei; Dong, Jing; Zou, Hanfa

    2014-08-25

    Hybrid monoliths with a macroporous structure were prepared within a few minutes via a photoinduced thiol-ene polymerization reaction, the surfaces of which showed hydrophobic character. The monolithic column demonstrated good separation performance towards alkylbenzenes, peptides, proteins and BSA tryptic digest in cLC.

  1. Efficient Functionalization of Oxide-Free Silicon(111) Surfaces: Thiol–yne versus Thiol–ene Click Chemistry

    NARCIS (Netherlands)

    Bhairamadgi, N.S.; Gangarapu, S.; Caipa Campos, M.A.; Paulusse, Jos Marie Johannes; van Rijn, C.J.M.; Zuilhof, H.

    2013-01-01

    Thiol-yne click (TYC) chemistry was utilized as a copper-free click reaction for the modification of alkyne-terminated monolayers on oxide-free Si(111) surfaces, and the results were compared with the analogous thiol–ene click (TEC) chemistry. A wide range of thiols such as

  2. Luuletus Halbdeutschis ja mõned küsimused substraadi kohta / Ilse Lehiste ; tlk. Ene-Reet Soovik

    Index Scriptorium Estoniae

    Lehiste, Ilse, 1922-2010

    2000-01-01

    Jacob Johann Malmi poolsaksakeelne satiiriline luuletus "Die Oberpalsche Freundschaft" aastast 1818 - luuletuse substraatkeelte eesti ja baltisaksa vokaalide ja konsonantsüsteemi ning grammatilise struktuuri võrdlus. Ilmunud: A poem in Halbdeutsch and some questions concerning substratum // Word 21, 1965, lk. 55-69. Tõlk. Ene-Reet Soovik

  3. Surface Modification of Poly(divinylbenzene) Microspheres via Thiol-Ene Chemistry and Alkyne-Azide Click Reactions

    NARCIS (Netherlands)

    Goldmann, Anja S.; Walther, Andreas; Nebhani, Leena; Joso, Raymond; Ernst, Dominique; Loos, Katja; Barner-Kowollik, Christopher; Barner, Leonie; Mueller, Axel H. E.; Müller, Axel H.E.

    2009-01-01

    We report the functionalization of cross-linked poly(divinylbenzene) (pDVB) microspheres using both thiol-ene chemistry and azide-alkyne click reactions. The RAFT technique was carried out to synthesize SH-functionalized poly(N-isopropylacrylimide) (pNIPAAm) and utilized to generate pNIPAAm

  4. Highly stretchable thermoset fibers and nonwovens using thiol-ene photopolymerization.

    Science.gov (United States)

    Shanmuganathan, Kadhiravan; Elliot, Steven M; Lane, Austin P; Ellison, Christopher J

    2014-08-27

    In this report, we describe the preparation and characterization of a new class of thermoset fibers with high elongation and elastic recovery. Integrating UV-activated thiol-ene photopolymerization and electrospinning, we demonstrate an environmentally friendly single step approach to convert small monomeric precursor molecules into highly elastic fibers and nonwoven mats. The fibers were derived by in situ photopolymerization of a trifunctional vinyl ether monomer and a tetrafunctional thiol. Although thermosets often offer good chemical and thermal stability, these fibers also have a high average elongation at break of 62%. The elastomeric nature of these vinyl-ether based fibers can be partly attributed to their subambient Tg and partly to the cross-link density, monomer structure, and resulting network homogeneity. Nonwoven mats of these fibers were also stretchable and exhibited a much higher elongation at break of about 85%. These thermoset stretchable fibers could have potential applications as textile, biomedical, hot chemical filtration, and composite materials.

  5. Photolithographic fabrication of solid–liquid core waveguides by thiol-ene chemistry

    DEFF Research Database (Denmark)

    Sagar, Kaushal Shashikant; Gopalakrishnan, Nimi; Christiansen, Mads Brøkner

    2011-01-01

    . The generated refractive index contrast between the patterned water-filled volume and the surrounding empty hydrophobic porous polymer allows for light confinement within the water-filled volume—the solid–liquid core. The presented fabrication process is simple and fast. It allows a high degree of flexibility...... are comparable with literature values for state-of-the-art liquid-core waveguide devices. The demonstrated waveguide function added to the nanoporous polymer with a very high internal surface area makes the system interesting for many applications in different areas, such as diagnostics and bio-chemical sensing.......In this work we demonstrate an efficient and cleanroom compatible method for the fabrication of solid–liquid core waveguides based on nanoporous polymers. We have used thiol-ene photo-grafting to tune and pattern the hydrophilicity of an originally hydrophobic nanoporous 1, 2-polybutadiene...

  6. Hydrophobic thiol-ene surfaces fabricated via plasma activation and photo polymerization

    Science.gov (United States)

    Champathet, P.; Ervithayasuporn, V.; Osotchan, T.; Dangtip, S.

    2017-09-01

    Alumina, such as glazed alumina for electrical insulator, operated in an open field subjects to a very harsh condition; resulting in lifetime shortening. Coating hydrophobic layer on alumina surface can help prolonging its lifetime. In this study, 25 ×25 mm alumina sheets were used as substrates. The hydrophobic composite polymers were prepared from (3-mercaptopropyl)trimethoxysilane(MPTMS), 2,4,6,8-tetramethyl-2,4,6,8tetravinylcyclotetra siloxane(TMTVSi), pentaerythritoltetra(3-mercaptopropionate)(PETMP), 2,2-dimethoxy-2-phe nylaceto phenone(photoinitiator) and heptadecafluorodecylmethacrylate(HEFDMA) via the thiol-ene reaction. The alumina sheets were first activated by dielectric-barrier discharge plasma to improve its adhesion. All the polymers were found to optimize at the ratio of (MPTMS:TMTVSi:PETMP:HDFDMA) to 4:2:1:2 for coating on the alumina substrate. To enhance polymerization, 2,2-dimethoxy-2-phenylaceto phenome was also used as a photoinitiator A proper mixing sequence in the thiol-ene reaction results in film with excellent surface retention after prolong soaking in solvent such as acetone. FTIR shows that S-H and C=C functional groups have significantly changed after photopolymerization and thermally cured. The static contact angle increase from mere 53.0°±1.5° of the uncoated substrate to 120.0°±1.2° after coating. SEM shows the film with clear appearance of a few-micron thick. Under AFM, the coated surface roughness was about 9.3 nm with evenly distributed spikes of a few nanometer in height. The cross-cut test also confirmed the film was very smooth and none of the square of the films detached.

  7. Hydrophobic Coatings by Thiol-Ene Click Functionalization of Silsesquioxanes with Tunable Architecture.

    Science.gov (United States)

    Dirè, Sandra; Bottone, Davide; Callone, Emanuela; Maniglio, Devid; Génois, Isabelle; Ribot, François

    2017-08-08

    The hydrolysis-condensation of trialkoxysilanes under strictly controlled conditions allows the production of silsesquioxanes (SSQs) with tunable size and architecture ranging from ladder to cage-like structures. These nano-objects can serve as building blocks for the preparation of hybrid organic/inorganic materials with selected properties. The SSQs growth can be tuned by simply controlling the reaction duration in the in situ water production route (ISWP), where the kinetics of the esterification reaction between carboxylic acids and alcohols rules out the extent of organosilane hydrolysis-condensation. Tunable SSQs with thiol functionalities (SH-NBBs) are suitable for further modification by exploiting the simple thiol-ene click reaction, thus allowing for modifying the wettability properties of derived coatings. In this paper, coatings were prepared from SH-NBBs with different architecture onto cotton fabrics and paper, and further functionalized with long alkyl chains by means of initiator-free UV-induced thiol-ene coupling with 1-decene (C10) and 1-tetradecene (C14). The coatings appeared to homogeneously cover the natural fibers and imparted a multi-scale roughness that was not affected by the click functionalization step. The two-step functionalization of cotton and paper warrants a stable highly hydrophobic character to the surface of natural materials that, in perspective, suggests a possible application in filtration devices for oil-water separation. Furthermore, the purification of SH-NBBs from ISWP by-products was possible during the coating process, and this step allowed for the fast, initiator-free, click-coupling of purified NBBs with C10 and C14 in solution with a nearly quantitative yield. Therefore, this approach is an alternative route to get sol-gel-derived, ladder-like, and cage-like SSQs functionalized with long alkyl chains.

  8. Amino acid-incorporated polymer network by thiol-ene polymerization

    Directory of Open Access Journals (Sweden)

    R. Yokose

    2015-08-01

    Full Text Available Triallyl L-alanine (A3A and triallyl L-phenylalanine (A3F were synthesized by reactions of L-alanine and L-phenylalanine with allyl bromide in the presence of sodium hydroxide, respectively. Thiol-ene thermal polymerization of A3A or A3F with pentaerythritol-based primary tetrathiol (pS4P or pentaerythritol-based secondary tetrathiol (S4P at allyl/SH 1/1 in the presence of 2,2'-azobis(isobutyronitrile produced an amino acid-incorporated polymer network (A3ApS4P, A3A-S4P or A3F-S4P. Although the thermally cured resins were homogeneous and flat films, the corresponding thiol-ene photopolymerization did not give a successful result. Degree of swelling for each thermally cured film in N,Ndimethylformamide was much higher than that in water. The glass transition and 5% weight loss temperatures (Tg and T5 of A3F-pS4P and A3F-S4P were higher than those of A3A-pS4P and A3A-S4P, respectively. Also, A3F-pS4P and A3F-S4P exhibited much higher tensile strengths and moduli than A3A-pS4P and A3A-S4P did, respectively. Consequently, A3FpS4P displayed the highest Tg (38.7°C, T5 (282.0°C, tensile strength (9.5 MPa and modulus (406 MPa among all the thermally cured resins.

  9. Swern Oxidation of Bicyclo[2.2.1]hept-5-ene-2,3-diol and Its Pyrazine-fused Derivatives: An Improved Synthesis of Bicyclo[2.2.1]hept-5-ene-2,3-dione and An Unexpected Ring-Opening Reaction

    Directory of Open Access Journals (Sweden)

    Sayuri Kobayashi

    2000-09-01

    Full Text Available An improved synthesis of bicyclo[2.2.1]hept-5-ene-2,3-dione by Swern oxidation of bicyclo[2.2.1]hept-5-ene-2,3-diol, and an unexpected ring-opening reaction by the Swern oxidation of pyrazine-fused congeners are described.

  10. Arhitektuur [1942-48] / Mart Kalm

    Index Scriptorium Estoniae

    Kalm, Mart, 1961-

    2013-01-01

    Arhitektuurikultuur. Taastamine. Sisult nõukogude, vormilt fašistlik. Modernismi jätkumisi. Kodu saavad valitud. Individuaalehitus kollektiivsuse utoopia taustal. Sunnitud või vabatahtlik monumentaalsus?

  11. Estonian parties face internal struggles / Helga Kalm

    Index Scriptorium Estoniae

    Kalm, Helga

    2010-01-01

    2011. aasta parlamendivalimiste eel on Eestimaa Rahvaliit, Erakond Eestimaa Rohelised ja Sotsiaaldemokraatlik Erakond keerulises olukorras. Parteides on probleeme juhatustega. Kahe esimese partei puhul ei ole üldse kindel, kas nad saavad parlamendis kohti või mitte. Tõnis Saartsi, Rein Toomla ja Andres Tarandi arvamusi

  12. Lasteaed = Day-care / Mart Kalm

    Index Scriptorium Estoniae

    Kalm, Mart, 1961-

    2013-01-01

    Lasteaedade ehitus Eestis algas 1920-ndatel aastatel. 1950-ndatest aastatest alates hakati lasteaedu ehitama tüüpprojektide järgi. Leidub ka üksikuid lasteaedu, mis pidid selle ehitanud ettevõtte jõukust ja hoolt demonstreerima

  13. Ene-yne Cross-Metathesis for the Preparation of 2,3-Diaryl-1,3-dienes

    Directory of Open Access Journals (Sweden)

    Meriem K. Abderrezak

    2017-11-01

    Full Text Available Ene-yne cross-metathesis from alkynes and ethylene is a useful method to produce substituted conjugated butadiene derivatives. If this method has been used with aliphatic alkynes, it has however never been used starting from diarylacetylenes as internal alkynes. We show that the ene-yne cross-metathesis catalyzed by the second generation Hoveyda ruthenium catalyst provides the 2,3-diarylbuta-1,3-dienes under 3 atm of ethylene at 100 °C. The scope and limitations of the reaction have been evaluated starting from unsymmetrical functionalized diarylacetylene derivatives hence leading to unsymmetrical 2,3-diarylbuta-1,3-dienes in a straightforward and environmentally acceptable manner.

  14. Emplazamiento hacia el ENE del manto de Los Reales (Alpujarrides occidentales: criterios estructurales y de petrofábrica

    Directory of Open Access Journals (Sweden)

    Tubía, J. M.

    1984-08-01

    Full Text Available Los Reales nappe rests over Ojén nappe to the North of Sierra Alpujata. The nappe contact zone shows a intense plastic deformation with mylonitic rocks, The deformation decreases progressively going away from the contacto The structural study of footwall and hangingwall roen permits to attribute this deformation to the Los Reales nappe emplacement over that of Ojén. The foliation and stretching lineation distributions show a WSW-ENE shear direction. The mylonite microstructural characteristics and the enstatite crystalline preferred orientations in the basal peridotites give a mear sense in according that, Los Reales nappe is displaced, in their present position, from the WSW towards the ENE.

    El manto de Los Reales descansa sobre el manto de Ojén, al norte de Sierra Alpujata. La zona de contacto entre mantos exhibe una intensa deformación plástica con rocas miloníticas; la deformación disminuye progresivamente al alejarse del contacto. El estudio estructural de las rocas infra y suprayacentes al contacto permite atribuir esta deformación al emplazamiento del manto de Los Reales sobre el de Ojén. , Las distribuciones de la foliación y de la lineación, de estiramiento indican una dirección de cizallamiento WSW-ENE. Las características microestructurales de las milonitas y las orientaciones preferentes cristalinas de la enstatita en las peridotitas basales proporcionan un sentido de cizallamiento tal que el manto de Los Reales se ha desplazado, en su posición actual, desde el WSW hacia el ENE.

  15. Vanemad mäletavad / Lain, Janet; Usin, Vello; Usin, Astra; Tiit, Birgit; Kuusk, Eneli; Kuusk, Vilja

    Index Scriptorium Estoniae

    2005-01-01

    Mõisaküla Keskkooli õpilased kogusid 2004. a. sügisel projekti "Mulgi murde päevad Mõisaküla koolis" raames vanemate inimeste mälestusi nende noorpõlvest ja kooliajast. Janet Lain pani kirja vanaisa Vello Usina ja vanaema Astra Usina mälestusi; Birgit Tiit ema Milvi Tiidu mälestusi ja Eneli Kuusk ema Vilja Kuuse mälestusi

  16. Aptamer-based organic-silica hybrid affinity monolith prepared via "thiol-ene" click reaction for extraction of thrombin.

    Science.gov (United States)

    Wang, Zheng; Zhao, Jin-cheng; Lian, Hong-zhen; Chen, Hong-yuan

    2015-06-01

    A novel strategy for preparing aptamer-based organic-silica hybrid monolithic column was developed via "thiol-ene" click chemistry. Due to the large specific surface area of the hybrid matrix and the simplicity, rapidness and high efficiency of "thiol-ene" click reaction, the average coverage density of aptamer on the organic-silica hybrid monolith reached 420 pmol μL(-1). Human α-thrombin can be captured on the prepared affinity monolithic column with high specificity and eluted by NaClO4 solution. N-p-tosyl-Gly-Pro-Arg p-nitroanilide acetate was used as the sensitive chromogenic substrate of thrombin. The thrombin enriched by this affinity column was detected with a detection of limit of 0.01 μM by spectrophotometry. Furthermore, the extraction recovery of thrombin at 0.15 μM in human serum was 91.8% with a relative standard deviation of 4.0%. These results indicated that "thiol-ene" click chemistry provided a promising technique to immobilize aptamer on organic-inorganic hybrid monolith and the easily-assembled affinity monolithic material could be used to realize highly selective recognition of trace proteins. Copyright © 2015 Elsevier B.V. All rights reserved.

  17. ENED-GEM: A Conceptual Framework Model for Psychological Enjoyment Factors and Learning Mechanisms in Educational Games about the Environment.

    Science.gov (United States)

    Fjællingsdal, Kristoffer S; Klöckner, Christian A

    2017-01-01

    Based on a thorough review of psychological literature, this article seeks to develop a model of game enjoyment and environmental learning (ENvironmental EDucational Game Enjoyment Model, ENED-GEM) and delineate psychological processes that might facilitate learning and inspire behavioral change from educational games about the environment. A critically acclaimed digital educational game about environmental issues (Fate of the World by Red Redemption/Soothsayer Games) was used as a case study. Two hundred forty-nine reviews of the game from the popular gaming and reviewing platform known as Steam were analyzed by means of a thematic content analysis in order to identify key player enjoyment factors believed to be relevant to the process of learning from games, as well as to gain an understanding of positive and negative impressions about the game's general content. The end results of the thematic analysis were measured up to the suggested ENED-GEM framework. Initial results generally support the main elements of the ENED-GEM, and future research into the importance of these individual core factors is outlined.

  18. ENED-GEM: A Conceptual Framework Model for Psychological Enjoyment Factors and Learning Mechanisms in Educational Games about the Environment

    Science.gov (United States)

    Fjællingsdal, Kristoffer S.; Klöckner, Christian A.

    2017-01-01

    Based on a thorough review of psychological literature, this article seeks to develop a model of game enjoyment and environmental learning (ENvironmental EDucational Game Enjoyment Model, ENED-GEM) and delineate psychological processes that might facilitate learning and inspire behavioral change from educational games about the environment. A critically acclaimed digital educational game about environmental issues (Fate of the World by Red Redemption/Soothsayer Games) was used as a case study. Two hundred forty-nine reviews of the game from the popular gaming and reviewing platform known as Steam were analyzed by means of a thematic content analysis in order to identify key player enjoyment factors believed to be relevant to the process of learning from games, as well as to gain an understanding of positive and negative impressions about the game’s general content. The end results of the thematic analysis were measured up to the suggested ENED-GEM framework. Initial results generally support the main elements of the ENED-GEM, and future research into the importance of these individual core factors is outlined. PMID:28701988

  19. Spray-deposition and photopolymerization of organic-inorganic thiol-ene resins for fabrication of superamphiphobic surfaces.

    Science.gov (United States)

    Xiong, Li; Kendrick, Laken L; Heusser, Hannele; Webb, Jamie C; Sparks, Bradley J; Goetz, James T; Guo, Wei; Stafford, Christopher M; Blanton, Michael D; Nazarenko, Sergei; Patton, Derek L

    2014-07-09

    Superamphiphobic surfaces, exhibiting high contact angles and low contact angle hysteresis to both water and low surface tension liquids, have attracted a great deal attention in recent years because of the potential of these materials in practical applications such as liquid-resistant textiles, self-cleaning surfaces, and antifouling/anticorrosion coatings. In this work, we present a simple strategy for fabricating of superamphiphobic coatings based on photopolymerization of hybrid thiol-ene resins. Spray-deposition and UV photopolymerization of thiol-ene resins containing hydrophobic silica nanoparticles and perfluorinated thiols provide a multiscale topography and low-energy surface that endows the surface with superamphiphobicity. The wettability and chemical composition of the surfaces were characterized by contact-angle goniometry and X-ray photoelectron spectroscopy, respectively. The hierarchical roughness features of the thiol-ene surfaces were investigated with field-emission scanning electron microscopy. Droplet impact and sandpaper abrasion tests indicate the coatings respectively possess a robust antiwetting behavior and good mechanical durability.

  20. Superhydrophobic and Slippery Lubricant-Infused Flexible Transparent Nanocellulose Films by Photoinduced Thiol-Ene Functionalization.

    Science.gov (United States)

    Guo, Jiaqi; Fang, Wenwen; Welle, Alexander; Feng, Wenqian; Filpponen, Ilari; Rojas, Orlando J; Levkin, Pavel A

    2016-12-14

    Films comprising nanofibrillated cellulose (NFC) are suitable substrates for flexible devices in analytical, sensor, diagnostic, and display technologies. However, some major challenges in such developments include their high moisture sensitivity and the complexity of current methods available for functionalization and patterning. In this work, we present a facile process for tailoring the surface wettability and functionality of NFC films by a fast and versatile approach. First, the NFC films were coated with a layer of reactive nanoporous silicone nanofilament by polycondensation of trichlorovinylsilane (TCVS). The TCVS afforded reactive vinyl groups, thereby enabling simple UV-induced functionalization of NFC films with various thiol-containing molecules via the photo "click" thiol-ene reaction. Modification with perfluoroalkyl thiols resulted in robust superhydrophobic surfaces, which could then be further transformed into transparent slippery lubricant-infused NFC films that displayed repellency against both aqueous and organic liquids with surface tensions as low as 18 mN·m-1. Finally, transparent and flexible NFC films incorporated hydrophilic micropatterns by modification with OH, NH2, or COOH surface groups, enabling space-resolved superhydrophobic-hydrophilic domains. Flexibility, transparency, patternability, and perfect superhydrophobicity of the produced nanocellulose substrates warrants their application in biosensing, display protection, and biomedical and diagnostics devices.

  1. Polysiloxane-based luminescent elastomers prepared by thiol-ene "click" chemistry.

    Science.gov (United States)

    Zuo, Yujing; Lu, Haifeng; Xue, Lei; Wang, Xianming; Wu, Lianfeng; Feng, Shengyu

    2014-09-26

    Side-chain vinyl poly(dimethylsiloxane) has been modified with mercaptopropionic acid, methyl 3-mercaptopropionate, and mercaptosuccinic acid. Coordinative bonding of Eu(III) to the functionalized polysiloxanes was then carried out and crosslinked silicone elastomers were prepared by thiol-ene curing reactions of these composites. All these europium complexes could be cast to form transparent, uniform, thin elastomers with good flexibility and thermal stability. The networks were characterized by FTIR, NMR, UV/Vis, and luminescence spectroscopy as well as by scanning electron microscopy, thermogravimetric analysis, and X-ray photoelectron spectroscopy. The europium elastomer luminophores exhibited intense red light at 617 nm under UV excitation at room temperature due to the (5)D0 →(7)F2 transition in Eu(III) ions. The newly synthesized luminescent materials offer many advantages, including the desired mechanical flexibility. They cannot be dissolved or fused, and so they have potential for use in optical and electronic applications. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  2. Thiol-Ene Clickable Gelatin: A Platform Bioink for Multiple 3D Biofabrication Technologies.

    Science.gov (United States)

    Bertlein, Sarah; Brown, Gabriella; Lim, Khoon S; Jungst, Tomasz; Boeck, Thomas; Blunk, Torsten; Tessmar, Joerg; Hooper, Gary J; Woodfield, Tim B F; Groll, Juergen

    2017-11-01

    Bioprinting can be defined as the art of combining materials and cells to fabricate designed, hierarchical 3D hybrid constructs. Suitable materials, so called bioinks, have to comply with challenging rheological processing demands and rapidly form a stable hydrogel postprinting in a cytocompatible manner. Gelatin is often adopted for this purpose, usually modified with (meth-)acryloyl functionalities for postfabrication curing by free radical photopolymerization, resulting in a hydrogel that is cross-linked via nondegradable polymer chains of uncontrolled length. The application of allylated gelatin (GelAGE) as a thiol-ene clickable bioink for distinct biofabrication applications is reported. Curing of this system occurs via dimerization and yields a network with flexible properties that offer a wider biofabrication window than (meth-)acryloyl chemistry, and without additional nondegradable components. An in-depth analysis of GelAGE synthesis is conducted, and standard UV-initiation is further compared with a recently described visible-light-initiator system for GelAGE hydrogel formation. It is demonstrated that GelAGE may serve as a platform bioink for several biofabrication technologies by fabricating constructs with high shape fidelity via lithography-based (digital light processing) 3D printing and extrusion-based 3D bioprinting, the latter supporting long-term viability postprinting of encapsulated chondrocytes. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  3. Iron complexes of tetramine ligands catalyse allylic hydroxyamination via a nitroso–ene mechanism

    Directory of Open Access Journals (Sweden)

    David Porter

    2015-12-01

    Full Text Available Iron(II complexes of the tetradentate amines tris(2-pyridylmethylamine (TPA and N,N′-bis(2-pyridylmethyl-N,N′-dimethylethane-1,2-diamine (BPMEN are established catalysts of C–O bond formation, oxidising hydrocarbon substrates via hydroxylation, epoxidation and dihydroxylation pathways. Herein we report the capacity of these catalysts to promote C–N bond formation, via allylic amination of alkenes. The combination of N-Boc-hydroxylamine with either FeTPA (1 mol % or FeBPMEN (10 mol % converts cyclohexene to the allylic hydroxylamine (tert-butyl cyclohex-2-en-1-yl(hydroxycarbamate in moderate yields. Spectroscopic studies and trapping experiments suggest the reaction proceeds via a nitroso–ene mechanism, with involvement of a free N-Boc-nitroso intermediate. Asymmetric induction is not observed using the chiral tetramine ligand (+-(2R,2′R-1,1′-bis(2-pyridylmethyl-2,2′-bipyrrolidine ((R,R′-PDP.

  4. Photo-initiated thiol-ene click reactions as a potential strategy for incorporation of [M(I)(CO)3]+ (M = Re, (99m)Tc) complexes.

    Science.gov (United States)

    Hayes, Thomas R; Lyon, Patrice A; Silva-Lopez, Elsa; Twamley, Brendan; Benny, Paul D

    2013-03-18

    Click reactions offer a rapid technique to covalently assemble two molecules. In radiopharmaceutical construction, these reactions can be utilized to combine a radioactive metal complex with a biological targeting molecule to yield a potent tool for imaging or therapy applications. The photo-initiated radical thiol-ene click reaction between a thiol and an alkene was examined for the incorporation of [M(I)(CO)3](+) (M = Re, (99m)Tc) systems for conjugating biologically active targeting molecules containing a thiol. In this strategy, a potent chelate system, 2,2'-dipicolylamine (DPA), for [M(I)(CO)3](+) was functionalized at the central amine with a terminal alkene linker that was explored with two synthetic approaches, click then chelate and chelate then click, to determine the flexibility and applicability of the thiol-ene click reaction to specifically incorporate ligand systems and metal complexes with a thiol containing molecule. In the click then chelate approach, the thiol-ene click reaction was carried out with the DPA chelate followed by complexation with [M(I)(CO)3](+). In the chelate then click approach, the alkene functionalized DPA chelate was first complexed with [M(I)(CO)3](+) followed by the conduction of the thiol-ene click reaction. Initial studies utilized benzyl mercaptan as a model thiol for both strategies to generate the identical product from either route to provide information on reactivity and product formation. DPA ligands functionalized with two unique linker systems (allyl and propyl allyl ether) were prepared to examine the effect of the proximity of the chelate or complex on the thiol-ene click reaction. Both the thiol-ene click and coordination reactions with Re, (99m)Tc were performed in moderate to high yields demonstrating the potential of the thiol-ene click reaction for [M(I)(CO)3](+) incorporation into thiol containing biomolecules.

  5. C27-C30 neohop-13(18)-enes and their saturated and aromatic derivatives in sediments: Indicators for diagenesis and water column stratification

    Science.gov (United States)

    Sinninghe Damsté, Jaap S.; Schouten, Stefan; Volkman, John K.

    2014-05-01

    A limited suite of C27, C29 and C30 rearranged hopenes identified as neohop-13(18)-enes have been reported in immature Recent and ancient marine/lacustrine sediments and their presence has been explained by dehydration and isomerisation of ubiquitous hopanols or hopenes. Here we investigated the source and fate of neohop-13(18)-enes in a range of Recent and ancient sediments. The analysis of δ13C values of hop-17(21)-ene and neohop-13(18)-ene in Arabian Sea surface sediments, in the Monterey Formation and in immature Cenomanian black shales show that they differ by 2-3‰, suggesting that the C30 neohop-13(18)-ene has a source different from those of the non-rearranged C30 hopenes. A new member of the family of neohop-13(18)-enes, the C28 hopene 28,30-dinorhop-13(18)-ene, was identified based on comparison of its mass spectral data with that of other members of the family of neohopenes. Its occurrence explains the formation of a series of orphan aromatic hopanoids bearing an ethyl group at C-21, known to occur in high concentrations in some organic-rich ancient sediments. Circumstantial evidence for this formation pathway is provided by identical δ13C values for the C28 28,30-dinorhop-13(18)-ene and two aromatic hopanoids in two Cretaceous black shales. Relatively abundant C28 28,30-dinorhopene and related aromatic derivatives were present in ancient sediments where the distribution of other biomarkers (i.e. isorenieratene derivatives) indicated a stratified palaeo water column. Therefore, it is suggested that these compounds are derived from bacteria dwelling at or below the chemocline and may be used as indicators of stratified water bodies in the past. 28,30-Dinorhop-13(18)-ene may also be a precursor of the unusual C28 desmethylhopane 28,30-dinorhopane found in high concentrations in anoxic sediments and a limited suite of crude oils, which is consistent with the proposal that it too ultimately derives from bacteria living at the oxic-anoxic interface.

  6. Synthesis and Transformations of di-endo-3-Aminobicyclo-[2.2.2]oct-5-ene-2-carboxylic Acid Derivatives

    Directory of Open Access Journals (Sweden)

    Márta Palkó

    2011-09-01

    Full Text Available all-endo-3-amino-5-hydroxybicyclo[2.2.2]octane-2-carboxylic acid (13 and all-endo-5-amino-6-(hydroxymethylbicyclo[2.2.2]octan-2-ol (10 were prepared via dihydro-1,3-oxazine or g-lactone intermediates by the stereoselective functionalization of an N-protected derivative of endo-3-aminobicyclo[2.2.2]oct-5-ene-2-carboxylic acid (2. Ring closure of b-amino ester 4 resulted in tricyclic pyrimidinones 15 and 16. The structures, stereochemistry and relative configurations of the synthesized compounds were determined by IR and NMR.

  7. Vabariigi presidendi, riigikogu esimehe ja peaministri ühisavaldus 8. mail 2007 / Toomas Hendrik Ilves, Ene Ergma, Andrus Ansip

    Index Scriptorium Estoniae

    Ilves, Toomas Hendrik, 1953-

    2007-01-01

    President Toomas Hendrik Ilvese, Riigikogu spiikri Ene Ergma ja peaminister Andrus Ansipi avaldus Teise maailmasõja lõpu 62. aastapäeva puhul. Ilmunud ka: Severnoje Poberežje 9. mai 2007, lk. 2; Eesti Elu 11. mai 2007, lk. 2, pealk.: Riigijuhtide ühisavaldus Teise maailmasõja mälestuspäeval, ingl. k. lk. 11, pealk.: Joint statement May 8, 2007; ingl. k. Vaba Eesti Sõna 10. mai 2007, lk. 12, pealk.: Joint statement of the President, Speaker of the Parliament and Prime Minister of the Republic of Estonia

  8. Thiol-ene and photo-cleavage chemistry for controlled presentation of biomolecules in hydrogels.

    Science.gov (United States)

    Grim, Joseph C; Marozas, Ian A; Anseth, Kristi S

    2015-12-10

    photochemical methods have led to important advances in the tissue regeneration community, namely the thiol-ene photo-click reaction for bioconjugation and photocleavage reactions that allow for the removal of protecting groups. Specific examples will be highlighted where these methodologies have been used to engineer hydrogels that control and direct cell function with the aim of inspiring their use in regenerative medicine. Copyright © 2015 Elsevier B.V. All rights reserved.

  9. A neutral polyacrylate copolymer coating for surface modification of thiol-ene microchannels for improved performance of protein separation by microchip electrophoresis

    DEFF Research Database (Denmark)

    Mesbah, Kiarach; Mai, T.D.; Jensen, Thomas Glasdam

    2016-01-01

    We have investigated the behavior of thiol-ene substrates that is a class of promising materials for lab-on-a-chip electrophoresis applications. Two polymeric materials were prepared by copolymerization of N, N-dimethylacrylamide (DMA), (3-(methacryloyl-oxy)propyl)trimethoxysilane (PMA) and 3......-(DMA-PMAMAPS) copolymer were evaluated in terms of surface hydrophilicity, suppression and stability of electro-osmotic flow and prevention of protein adsorption. Surface modification of thiol-ene containing a 20 % excess of thiols with the terpolymer p-(DMA-PMA-MAPS) was found to offer the most stable coating and most...

  10. [Preparation of organic-inorganic hybrid boronate affinity monolith via thiol-ene click reaction for specific capture of glycoproteins].

    Science.gov (United States)

    Yang, Fan; Mao, Jie; He, Xiwen; Chen, Langxing; Zhang, Yukui

    2013-06-01

    A novel strategy for the preparation of the organic-inorganic hybrid boronate affinity monolith was developed via the "thiol-ene" click reaction. A thiol group-modified silica monolith was first synthesized via the sol-gel process by the in situ co-condensation with tetramethoxysilane (TMOS) and 3-mercaptopropyltrimethoxysilane (MPTMS) as precursors. Then 3-acrylamidophenylboronic acid (AAPBA) was covalently immobilized on the hybrid monolith via the "thiol-ene" click reaction to form AAPBA-silica hybrid affinity monolith. The reaction conditions for the preparation of AAPBA-silica hybrid affinity monolith were optimized, including the ratio of TMOS to MPTMS, the contents of poly(ethylene glycol) (PEG) and methanol. The morphology and mechanical stability of the boronate affinity monolith were characterized and evaluated by scanning electron microscopy and Fourier-transform infrared spectroscopy. The obtained boronate affinity hybrid monolith exhibited excellent specificity toward the nucleosides containing cis-diols under neutral conditions. It was further applied to the specific capture of the glycoproteins ovalbumin and horseradish peroxidase. The method is novel and reliable, which has a great potential for the preparation of different kinds of the boronate affinity monoliths.

  11. One-pot preparation of an organic polymer monolith by thiol-ene click chemistry for capillary electrochromatography.

    Science.gov (United States)

    Wang, Jiannan; Shen, Shufen; Lu, Xin; Ye, Fanggui

    2017-08-01

    A novel organic monolith was successfully fabricated by a one-pot thiol-ene click reaction of triallyl isocyanurate with pentaerythritol tetrakis-(2-mercaptoacetate) and mercaptopropionic acid in the presence of porogens. We investigated the effects of the ratio of monomer and cross-linking agent, the type and ratio of porogen, and click reaction temperature on the permeability and morphology of the prepared poly triallyl isocyanurate-co-pentaerythritol tetrakis (2-mercaptoacetate) monoliths. The monolith was also characterized by scanning electron microscopy and Fourier transform infrared spectroscopy. The results indicated that the monoliths had continuous porous framework, good permeability, and high mechanical stability. A series of analytes with different properties such as alkylbenzenes, polycyclic aromatic hydrocarbons, anilines, and phenols were used to evaluate the electrochromatographic performance of the prepared monoliths in pressurized capillary electrochromatography. The prepared polymer monolith showed typical reversed-phase electrochromatographic behavior for hydrophobic substances. Moreover, the prepared monolith showed a mix of reversed-phase and cation exchange interaction modes for basic aniline compounds. The minimum plate height of the monolith was 8.76 μm (132 100 plates/m) for propylbenzene. These results demonstrated that one-pot thiol-ene click chemistry can provide a simple and reliable method for the preparation of organic monoliths. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  12. A colorimetric detection of acrylamide in potato chips based on nucleophile-initiated thiol-ene Michael addition.

    Science.gov (United States)

    Hu, Qinqin; Fu, Yingchun; Xu, Xiahong; Qiao, Zhaohui; Wang, Ronghui; Zhang, Ying; Li, Yanbin

    2016-02-07

    Acrylamide (AA), a neurotoxin and a potential carcinogen, has been found in various thermally processed foods such as potato chips, biscuits, and coffee. Simple, cost-effective, and sensitive methods for the rapid detection of AA are needed to ensure food safety. Herein, a novel colorimetric method was proposed for the visual detection of AA based on a nucleophile-initiated thiol-ene Michael addition reaction. Gold nanoparticles (AuNPs) were aggregated by glutathione (GSH) because of a ligand-replacement, accompanied by a color change from red to purple. In the presence of AA, after the thiol-ene Michael addition reaction between GSH and AA with the catalysis of a nucleophile, the sulfhydryl group of GSH was consumed by AA, which hindered the subsequent ligand-replacement and the aggregation of AuNPs. Therefore, the concentration of AA could be determined by the visible color change caused by dispersion/aggregation of AuNPs. This new method showed high sensitivity with a linear range from 0.1 μmol L(-1) to 80 μmol L(-1) and a detection limit of 28.6 nmol L(-1), and especially revealed better selectivity than the fluorescence sensing method reported previously. Moreover, this new method was used to detect AA in potato chips with a satisfactory result in comparison with the standard methods based on chromatography, which indicated that the colorimetric method can be expanded for the rapid detection of AA in thermally processed foods.

  13. Synthesis of antibacterial amphiphilic elastomer based on polystyrene-block-polyisoprene-block-polystyrene via thiol-ene addition

    Energy Technology Data Exchange (ETDEWEB)

    Keleş, Elif, E-mail: elifkelesh@hotmail.com [Department of Chemistry, Bülent Ecevit University, Zonguldak 67100 (Turkey); Hazer, Baki, E-mail: bhazer2@yahoo.com [Department of Chemistry, Bülent Ecevit University, Zonguldak 67100 (Turkey); Cömert, Füsun B. [Department of Microbiology, Faculty of Medicine, Bülent Ecevit University, 67600 Zonguldak (Turkey)

    2013-04-01

    A new type of amphiphilic antibacterial elastomer has been described. Thermoplastic elastomer, polystyrene–block-polyisoprene–block-polystyrene (PS–b-PI–b-PS) triblock copolymer was functionalized in toluene solution by free radical mercaptan addition in order to obtain an amphiphilic antibacterial elastomer. Thiol terminated PEG was grafted through the double bonds of PS–b-PI–b-PS via free radical thiol-ene coupling reaction. The antibacterial properties of the amphiphilic graft copolymers were observed. The original and the modified polymers were used to create microfibers in an electro-spinning process. Topology of the electrospun micro/nanofibers were studied by using scanning electron microscopy (SEM). The chemical structures of the amphiphilic comb type graft copolymers were elucidated by the combination of elemental analysis, {sup 1}H NMR, {sup 13}C NMR, GPC and FTIR. - Graphical abstract: Double bonds of polyisoprene units in polystyrene–block-polyisoprene–block-polystyrene triblock copolymer were partially capped with PEG containing mercapto end group via thiol-ene addition in order to obtain antibacterial amphiphilic elastomer. Nano fibers from amphiphilic graft polymers solution were produced by electrospinning. The PEG grafted copolymer inhibits very effectively bacterial growth. Highlights: ► A commercial synthetic elastomer was grafted with PEG to obtain amphiphilic elastomer. ► Amphiphilic elastomer shows antibacterial properties. ► Electrospun micro fibers of the amphiphilic elastomer tend to globular formation.

  14. Functional characterization and stability improvement of a ‘thermophilic-like’ ene-reductase from Rhodococcus opacus 1CP

    Directory of Open Access Journals (Sweden)

    Anika eRiedel

    2015-10-01

    Full Text Available Ene-reductases are widely applied for the asymmetric synthesis of relevant industrial chemicals. A novel ene-reductase OYERo2 was found within a set of 14 putative Old Yellow Enzymes (OYEs obtained by genome mining of the actinobacterium Rhodococcus opacus 1CP. Multiple sequence alignment suggested that the enzyme belongs to the group of ‘thermophilic-like’ OYEs. OYERo2 was produced in Escherichia coli and biochemically characterized. The enzyme is strongly NADPH dependent and uses non-covalently bound FMNH2 for the reduction of activated α,β-unsaturated alkenes. In the active form OYERo2 is a dimer. Optimal catalysis occurs at pH 7.3 and 37 °C. OYERo2 showed highest specific activities (4550 U mg-1 on maleimides, which are efficiently converted to the corresponding succinimides. The OYERo2-mediated reduction of prochiral alkenes afforded the (R-products with excellent optical purity (ee > 99%. OYERo2 is not as thermo-resistant as related OYEs. Introduction of a characteristic intermolecular salt bridge by site-specific mutagenesis raised the half-life of enzyme inactivation at 32 °C from 28 min to 87 min and improved the tolerance towards organic co-solvents. The suitability of OYERo2 for application in industrial biocatalysis is discussed.

  15. Statistical effects in the thermal deazetization reaction of 2,3-diazabicyclo(2.2.1)hept-2-ene

    Science.gov (United States)

    Sorescu, Dan C.; Thompson, Donald L.; Raff, Lionel M.

    1995-10-01

    The deazetization reaction of 2,3-diazabicyclo(2.2.1)hept-2-ene-exo, exo-5,6-d2 is investigated using Monte Carlo classical variational transition-state theory, implemented by the efficient microcanonical sampling procedure. Comparison is made with the results of trajectory calculations performed on the same global potential-energy surface. The microcanonical reaction rates have been determined for both reaction channels, i.e., the stepwise and concerted cleavage of the two C-N bonds of the reactant. The results demonstrate that the thermal decomposition of 2,3-diazabicyclo (2.2.1)hept-2-ene-exo,exo-5,6-d2 is well described by statistical theories that assume equal weighting for all energetically accessible phase-space points. It is found that the rate coefficients of the statistical calculations are close upper bounds of the rates determined in trajectory calculations. Previously reported classical trajectory simulations have shown that the distribution of internal energy in the reactant configuration, at the transition state and beyond is very nearly microcanonical for the range of excitation energies analyzed (60-175 kcal/mol). Under such conditions, the agreement obtained between the present statistically computed rate coefficients and those extracted from the trajectory results is not surprising. It is suggested that nonstatistical post-transition-state events account for the nonunity ratio of the exo/endo reaction products observed experimentally. These events are not considered in the present statistical theories of the reaction rates.

  16. Molecular structure and conformations of 1-methoxy-2,3,3,4,4-pentafluorocyclobut-1-ene

    Science.gov (United States)

    Frogner, Matthew; Hedberg, Kenneth; Hedberg, Lise; Lunelli, Bruno

    2010-08-01

    The structure of 1-monomethoxy-2,3,3,4,4-pentafluorocyclobut-1-ene (F5MCB) has been studied as a part of a series of investigations of the structures of substituted cyclobutenes. The experimental method was gas-phase electron diffraction (GED) augmented by molecular orbital calculations at the HF and B3LYP levels of theory with basis sets 6-31G(d), 6-311+G(3df,2p), and cc-pvtZ. The matter of most interest in these compounds is the length of the C3-C4 bond, which has been found to be significantly longer than the corresponding bond in cyclobutene itself when both ligands to the double bond are fluorine or chlorine atoms, but about the same as in cyclobutene when both substituents are methoxy groups. This result has been verified by the additional data provided by this study of F5MCB: the C3-C4 bond is about the same as in the dimethoxy compound and in cyclobutene. In the gas-phase at 25 °C, F5MCB exists as a mixture of two conformers, one with the methyl group cis to the double bond (about 70%) and one with it trans (about 30%), in good agreement with theory that predicts 63-70% cis. Some values (with estimated 2 σ uncertainties) of the more important parameters ( rg/Å; ∠ α/°) are for the cis/ trans forms r() = 1.101 (17)/1.121, r(C dbnd C) = 1.329 (36)/1.328, r(C1-C4) = 1.498 (19)/1.504, r(C2-C3) = 1.483 (19)/1.485, r(C3-C4) = 1.581 (21)/1.576, r(C2-F6)=1.346 (12)/1.339, r(C3-F7) = 1.347 (10)/1.349, r(C4-F9) = 1.344 (10)/1.350, r(C1-O5) = 1.337 (26)/1.343, r(O5-C11) = 1.469 (37)/1.493, ∠(C4C1C2) = 93.3 (8)/93.1, ∠(C1C2C3) = 96.4 (8)/96.6, ∠(C2C3C4) = 84.4 (8)/84.4, ∠(C3C4C1) = 85.0 (9)/85.9, ∠(C2C1O5) = 141.0 (14)/135.2, ∠(C1C2F6) = 135.4 (14)/135.1, ∠(C1O5C11) = 117.3 (33)/117.8, ∠(F9C4F10) = 108.3 (13)/107.7, ∠(F7C3F8) = 107.7 (13)/107.6, ∠(XC4C3) = 134.4 (15)/134.4, and ∠(X'C3C4) = 134.2 (15)/133.9, where X and X' are, respectively, the bisectors of the F9C4F10 and F7C3F8 angles. The structure is discussed with reference to the

  17. Combining ring-opening metathesis polymerization and thiol-ene coupling chemistries: facile access to novel functional linear and nonlinear macromolecules.

    Science.gov (United States)

    Lowe, Andrew B; Liu, Meina; van Hensbergen, Johannes Arend; Burford, Robert P

    2014-02-01

    The aim of this article is to highlight recent examples in which two powerful synthetic tools, namely ring-opening metathesis polymerization (ROMP) and thiol-ene (including the thiol-Michael variant) click chemistry have been combined to facilitate the preparation of novel functional materials of varying topology. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  18. Azaspirene: a novel angiogenesis inhibitor containing a 1-oxa-7-azaspiro[4.4]non-2-ene-4,6-dione skeleton produced by the fungus Neosartorya sp.

    Science.gov (United States)

    Asami, Yukihiro; Kakeya, Hideaki; Onose, Rie; Yoshida, Arika; Matsuzaki, Hiroshi; Osada, Hiroyuki

    2002-08-22

    [structure: see text] Azaspirene isolated from the fungus Neosartorya sp. is a novel angiogenesis inhibitor with a 1-oxa-7-azaspiro[4.4]non-2-ene-4,6-dione skeleton. Azaspirene inhibits the endothelial migration induced by vascular endothelial growth factor (ED100 = 27.1 microM).

  19. N-(3,4-Dimethoxyphenyl-4-oxo-2,3,7-triazaspiro[4.5]dec-1-ene-1-carboxamide Hydrochloride

    Directory of Open Access Journals (Sweden)

    Seranthimata Samshuddin

    2012-08-01

    Full Text Available A simple and novel route for synthesis of new spirocyclic amide derivative is developed. The present work involves the oxidative cleavage of tert-butyl 1-(furan-2-yl-4-oxo-2,3,7-triazaspiro[4.5]dec-1-ene-7-carboxylate 1 followed by the amine coupling and deprotection of Boc.

  20. Noorsootöö Keskusel täitub kümnes tegevusaasta / Edgar Schlümmer ; kommenteerinud Irma Toodo, Ene Raid, Andres Lokk

    Index Scriptorium Estoniae

    Schlümmer, Edgar

    2009-01-01

    Eesti Noorsootöö Keskus (ENTK)tähistab kümnendat sünnipäeva. ENTK rolli tänapäeva noorsootöös kommenteerivad endine ENTK töötaja Irma Toodo ja praegused töötajad Ene Raid ning Andres Lokk

  1. Ene-Liis Semper: selle aasta puhkepäevad võib ühe käe sõrmedel kokku lugeda

    Index Scriptorium Estoniae

    Semper, Ene-Liis, 1969-

    2011-01-01

    Tallinna kultuuripealinna projekti panustanud Ene-Liis Semper võtab 2011. aasta sündmused kokku ja selgitab, kuidas jõudsid publiku ette suurlavastused "The Rise and Fall of Estonia", "Three Kingdoms", Põhuteater ning isikunäitus Kumus

  2. (E-2-((4R,5R-5-((Benzyloxymethyl-2,2-dimethyl-1,3-dioxolan-4-ylbut-2-ene-1,4-diol

    Directory of Open Access Journals (Sweden)

    Carlos R. Carreras

    2010-04-01

    Full Text Available The synthesis of (E-2-((4R,5R-5-((benzyloxymethyl-2,2-dimethyl-1,3-dioxolan-4-ylbut-2-ene-1,4-diol by a one-step reduction of the appropriate 2-substituted butenolide is reported. Product characterization was carried out by IR, 1H NMR, 13C NMR, MS, elemental analysis and optical rotation.

  3. Joint statement of the President, Speaker of the Riigikogu and Prime Minister of the Republic of Estonia / Toomas Hendrik Ilves, Ene Ergma, Andrus Ansip

    Index Scriptorium Estoniae

    Ilves, Toomas Hendrik, 1953-

    2008-01-01

    President Toomas Hendrik Ilvese, Riigikogu spiikri Ene Ergma ja peaminister Andrus Ansipi ühisavaldus Teise maailmasõja lõpu 62. aastapäeva puhul 8. mail 2007, milles kinnitatakse, et hoolimata Teise maailmasõja ajaloolistest tõlgendustest on kõik Eestis elavad inimesed vabad ning jagavad ühist tulevikku

  4. Broadening the scope of Baeyer-Villiger monooxygenase activities toward α,β-unsaturated ketones: a promising route to chiral enol-lactones and ene-lactones.

    Science.gov (United States)

    Reignier, T; de Berardinis, V; Petit, J-L; Mariage, A; Hamzé, K; Duquesne, K; Alphand, V

    2014-07-25

    Three regiodivergent Baeyer-Villiger mono-oxygenases (enantioselectively) oxidized a series of cyclic α,β-unsaturated ketones into (chiral) either enol-lactones or ene-lactones. An easy-to-use and efficient biocatalytic process based on a host-microorganism deprived of unwanted activities (knock-out mutant) was developed to enable the exclusive synthesis of unsaturated lactones.

  5. C27-C30 neohop-13(18)-enes and their saturated and aromatic derivatives in sediments: Indicators for diagenesis and water column stratification

    NARCIS (Netherlands)

    Sinninghe Damsté, J.S.; Schouten, S.; Volkman, J.K.

    2014-01-01

    A limited suite of C27, C29 and C30 rearranged hopenes identified as neohop-13(18)-enes have been reported in immature Recent and ancient marine/lacustrine sediments and their presence has been explained by dehydration and isomerisation of ubiquitous hopanols or hopenes. Here we investigated the

  6. 1,25-(OH)2-16ene-23yne-D3 reduces secondary hyperparathyroidism in uremic rats with little calcemic effect.

    Science.gov (United States)

    Lippuner, Kurt; Perrelet, Romain; Casez, Jean-Paul; Popp, Albrecht; Uskokovic, Milan R; Jaeger, Philippe

    2004-01-01

    To compare the effects of vitamin D analogs versus calcitriol on serum levels of Ca, P and parathyroid hormone (PTH). A compound better than calcitriol should increase the Ca x P product less than calcitriol for an equivalent decrease in PTH levels. Biological activity of 4 vitamin D analogs, 1,25-(OH)(2)-16ene- D(3) (RO(1)), 1,25-(OH)(2)-16ene-23yne-D(3) (RO(2)), 1,25-(OH)(2)-26,27-hexafluoro-16ene-23yne-D(3) (RO(3)) and 1,25-(OH)(2)-16ene-23yne-26,27-hexafluoro-19nor-D(3) (RO(4)) was tested vs. calcitriol in parathyroidectomized rats. In a second set of experiments, the effects of RO(2), RO(4) and calcitriol were studied in 5/6 nephrectomized rats with secondary hyperparathyroidism. In parathyroidectomized rats, all analogs (250 pmol/day) led calcemia to rise after 7 days. In uremic rats, all treatments reduced PTH levels. RO(4) revealed toxicity. RO(2) was as effective as calcitriol in suppressing PTH in a dose dependent manner. Mean plasma ionized calcium did not change from baseline to day 14 and day 28 on RO(2) (250 or 500 pmol/day) whereas it increased significantly on RO(2) (1,000 pmol/day) and calcitriol (125 or 250 pmol/day). Increasing the dose of calcitriol led Ca x P to rise more dramatically than increasing the dose of RO(2), which appears to have a wider therapeutic window than calcitriol. 1,25-(OH)(2)-16ene-23yne-D(3) (RO(2)) may represent a novel candidate for the treatment of renal osteodystrophy in humans. Copyright 2004 S. Karger AG, Basel

  7. Synthesis and preliminary bioevaluation of 99mTc(CO)3-17α-triazolylandrost-4-ene-3-one derivative prepared via click chemistry route.

    Science.gov (United States)

    Dhyani, Manish V; Satpati, Drishty; Korde, Aruna; Banerjee, Sharmila

    2011-10-01

    Azolyl steroids are known to manifest antiprostate cancer and antiandrogenic activities. These azolyl steroids have been shown to express affinity toward androgen receptors (ARs) overexpressed on LNCaP (human prostate adenocarcinoma) cell line. Hence, suitably derivatized azolyl steroids can be envisaged as potential vectors for targeting overexpression of ARs in prostate cancer. In the present study, testosterone has been derivatized to 17α-azidoandrost-4-ene-3-one using microwave-mediated azidation of the mesylate. Subsequently, a facile one-pot Cu(I)-catalyzed Click reaction was carried out to synthesize (99m)Tc(CO)(3)-labeled 17α-triazolylandrost-4-ene-3-one, which was characterized by HPLC. The chemical characterization of (99m)Tc(CO)(3)-17α-triazolylandrost-4-ene-3-one was carried out by preparing its corresponding rhenium complex using [NEt(4)](2)[Re(CO)(3)Br(3)] precursor. The radiolabeled complex could be prepared in >95% radiochemical yield as determined by HPLC. In vitro studies of (99m)Tc(CO)(3)-17α-triazolylandrost-4-ene-3-one complex in LNCaP cell lines overexpressing ARs showed binding of 4.95%±1.2%, with inhibition of 8%±0.9%. In vivo biodistribution studies in male Wistar rats have shown uptake in the prostate to the extent of 0.48%±0.19% injected dose/g at 1 hpi and retention therein till 3 hpi. The present study demonstrates a novel and facile one-pot reaction for preparation of (99m)Tc-labeled 17α-triazolylandrost-4-ene-3-one complex using Click chemistry. The corresponding Re-analog has been prepared for purpose of comparative characterization with the (99m)Tc-labeled complex. The radiosynthetic strategy described in this article can be further extended toward preparation of radiolabeled complexes of other triazolyl steroidal derivatives.

  8. The preparation and physical properties of polysulfide-based elastomers through one-pot thiol-ene click reaction

    Directory of Open Access Journals (Sweden)

    Y. W. Quan

    2013-07-01

    Full Text Available In this paper, polysulfide-based elastomers were successfully prepared through a simple one-pot thiol-ene click reaction of the liquid polysulfide oligomer with bisphenol-A diacrylate resin. Real-time Fourier transform infrared spectroscopy (FTIR analysis showed that the molecular weight of the liquid polysulfide oligomer had no effect on mercaptan functional group conversion. The obtained elastomers continued to keep low temperature flexibility of polysulfide except Elastomer-LP3, which was due to higher content of bisphenol-A structure. All the samples had a tensile strength of over 0.7 MPa, which was comparable to that of polysulfide polymer cured by metal oxide. Moreover, the samples exhibited higher thermal stability than metal oxide cured polysulfide. This vulcanization methodology will provide a fast, efficient, and environmentally friendly approach (without metal oxides and plasticizers for preparing polysulfide elastomers.

  9. Modifying surface resistivity and liquid moisture management property of keratin fibers through thiol-ene click reactions.

    Science.gov (United States)

    Yu, Dan; Cai, Jackie Y; Church, Jeffrey S; Wang, Lijing

    2014-01-22

    This paper reports on a new method for improving the antistatic and liquid moisture management properties of keratinous materials. The method involves the generation of thiols by controlled reduction of cystine disulfide bonds in keratin with tris(2-carboxyethyl) phosphine hydrochloride and subsequent grafting of hydrophilic groups onto the reduced keratin by reaction with an acrylate sulfonate or acrylamide sulfonate through thiol-ene click chemistry. The modified substrates were characterized with Raman spectroscopy and scanning electron microscopy and evaluated for their performance changes in liquid moisture management, surface resistivity, and wet burst strength. The results have revealed that the thiol-acrylate reaction is more efficient than the thiol-acrylamide reaction, and the keratinous substrate modified with an acrylate sulfonate salt exhibits significantly improved antistatic and liquid moisture management properties.

  10. Preparation of a novel carboxyl stationary phase by "thiol-ene" click chemistry for hydrophilic interaction chromatography.

    Science.gov (United States)

    Peng, Xi-Tian; Liu, Tao; Ji, Shu-Xian; Feng, Yu-Qi

    2013-08-01

    A novel carboxyl-bonded silica stationary phase was prepared by "thiol-ene" click chemistry. The resultant Thiol-Click-COOH phase was evaluated under hydrophilic interaction liquid chromatography (HILIC) mobile phase conditions. A comparison of the chromatographic performance of Thiol-Click-COOH and pure silica columns was performed according to the retention behaviors of analytes and the charged state of the stationary phases. The results indicated that the newly developed Thiol-Click-COOH column has a higher surface charge and stronger hydrophilicity than the pure silica column. Furthermore, the chromatographic behaviors of five nucleosides on the Thiol-Click-COOH phase were investigated in detail. Finally, a good separation of 13 nucleosides and bases, and four water-soluble vitamins was achieved. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  11. Orthogonal dual thiol–chloroacetyl and thiol–ene couplings for the sequential one-pot assembly of heteroglycoclusters

    Directory of Open Access Journals (Sweden)

    Michele Fiore

    2014-07-01

    Full Text Available We describe the first one-pot orthogonal strategy to prepare well-defined cyclopeptide-based heteroglycoclusters (hGCs from glycosyl thiols. Both thiol–chloroactetyl coupling (TCC and thiol–ene coupling (TEC have been used to decorate cyclopeptides regioselectively with diverse combination of sugars. We demonstrate that the reaction sequence starting with TCC can be performed one-pot whereas the reverse sequence requires a purification step after the TEC reaction. The versatility of this orthogonal strategy has been demonstrated through the synthesis of diverse hGCs displaying alternating binary combinations of α-D-Man or β-D-GlcNAc, thus providing rapid access to attractive heteroglycosylated platforms for diverse biological applications.

  12. Photoinduced Thiol-ene Chemistry Applied to the Synthesis of Self-Assembling Elastin-Inspired Glycopeptides.

    Science.gov (United States)

    Piccirillo, Germano; Pepe, Antonietta; Bedini, Emiliano; Bochicchio, Brigida

    2017-02-21

    Synthetic (glyco)peptides inspired by proteins able to self-assemble are appealing biomaterials in the field of tissue engineering and regenerative medicine. Herein, for the first time, taking advantage of thiol-ene chemistry coupled to solid-phase peptide synthesis, a self-assembling peptide inspired by elastin protein was bioconjugated to three carbohydrates in order to obtain the corresponding glycopeptides. They were studied at the molecular and supramolecular level. The results show that the carbohydrate influences the molecular conformation of the glycopeptide and its self-aggregation properties as well. As future perspective, the results could enable us to tune the final self-aggregation properties of the glycopeptide by changing the sugar moiety. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  13. Stereochemistry of 16a-Hydroxyfriedelin and 3-Oxo-16-methylfriedel-16-ene Established by 2D NMR Spectroscopy

    Directory of Open Access Journals (Sweden)

    Vagner Fernandes Knupp

    2009-02-01

    Full Text Available Friedelin (1, 3b-friedelinol (2, 28-hydroxyfriedelin (3, 16a-hydroxyfriedelin (4, 30-hydroxyfriedelin (5 and 16a,28-dihydroxyfriedelin (6 were isolated through fractionation of the hexane extract obtained from branches of Salacia elliptica. After a week in CDCl3 solution, 16a-hydroxyfriedelin (4 reacted turning into 3-oxo-16-methylfriedel-16-ene (7. This is the first report of a dehydration followed by a Nametkin rearrangement of a pentacyclic triterpene in CDCl3 solution occurring in the NMR tube. These seven pentacyclic triterpenes was identified through NMR spectroscopy and the stereochemistry of compound 4 and 7 was established by 2D NMR (NOESY spectroscopy and mass spectrometry (GC-MS. It is also the first time that all the 13C-NMR and 2D NMR spectral data are reported for compounds 4 and 7.

  14. Molecular dynamics studies of the thermal decomposition of 2,3-diazabicyclo(2.2.1)hept-2-ene

    Science.gov (United States)

    Sorescu, Dan C.; Thompson, Donald L.; Raff, Lionel M.

    1995-05-01

    The reaction dynamics of the thermal gas-phase decomposition of 2,3-diazabicyclo (2.2.1)hept-2-ene-exo, exo-5,6-d2 have been investigated using classical trajectory methods on a semiempirical potential-energy surface. The global potential is written as a superposition of different reaction channel potentials containing bond stretching, bending and torsional terms, connected by parametrized switching functions. Reaction channels for stepwise and concerted cleavage of the two C-N bonds of the reactant have both been considered in construction of the potential. The geometries of 2,3-diazabicyclo(2.2.1)hept-2-ene, the diazenyl biradical and of the transition state corresponding to breaking of the remaining C-N bond of diazenyl biradical have been determined at the second order Möller-Plesset perturbation theory (MP2/6-31G*) and at Hartree-Fock (HF/6-31G*) levels, respectively. The bond dissociation energies have been estimated using the available thermochemical data and previously reported results for bicyclo(2.1.0)pentane [J. Chem. Phys. 101, 3729 (1994)]. The equilibrium geometries predicted by the semiempirical potential for reactants and products, the barrier height for thermal nitrogen extrusion from 2,3-diazabicyclo(2.2.1)hept-2-ene and the fundamental vibrational frequencies are in good to excellent agreement with the measured or ab initio calculated values. Using a projection method of the instantaneous Cartesian velocities onto the normal mode vectors and classical trajectory calculations, the dissociation dynamics of 2,3-diazabicyclo(2.2.1)hept-2-ene-exo, exo-5,6-d2 are investigated at several excitation energies in the range 60-175 kcal/mol. The results show the following: (1) The thermal reaction takes place with a preference for inversion of configuration in the reaction products, the exo-labeled bicyclo(2.1.0) pentane being the major product. The exo/endo ratio of bicyclo(2.1.0) pentane isomers is found to vary between 1.8-2.2 for the energy range

  15. Unexpected Carbon-Carbon Coupling between Organic Cyanides and an Isopropyl β-Carbon in a Hafnium Ene Diamide Complex

    OpenAIRE

    Bol, Johan E.; Hessen, Bart; Teuben, Jan H.; Smeets, Wilberth J.J.; Anthony L. Spek

    1992-01-01

    A reaction sequence involving two hydrogen transfers and a C-C coupling on the β-carbon of an isopropyl group leads to formation of a new dianionic tridentate ligand in the reaction of the ene diamide complex Cp*Hf(σ2,π-iPr-DAB)Cl (1; Cp* = η5-C5Me5, iPr-DAB = 1,4-diisopropyl-1,4-diaza-1,3-butadiene) with organic cyanides. The product Cp*Hf [iPrNCH=CHNC(Me)=CHC(tBu)=NH]Cl was structurally characterized (Pbca, a = 13.454 (1) Å, b = 11.470 (1) Å, c = 31.297 (2) Å, 100 K). The reaction sequence ...

  16. Facile preparation of organic-silica hybrid monolith for capillary hydrophilic liquid chromatography based on "thiol-ene" click chemistry.

    Science.gov (United States)

    Chen, Ming-Luan; Zhang, Jun; Zhang, Zheng; Yuan, Bi-Feng; Yu, Qiong-Wei; Feng, Yu-Qi

    2013-04-05

    In this work, a one-step approach to facile preparation of organic-inorganic hybrid monoliths was successfully developed. After vinyl-end organic monomers and azobisisobutyronitrile (AIBN) were mixed with hydrolyzed tetramethoxysilane (TMOS) and 3-mercaptopropyltrimethoxysilane (MPTMS), the homogeneous mixture was introduced into a fused-silica capillary for simultaneous polycondensation and "thiol-ene" click reaction to form the organic-silica hybrid monoliths. By employing this strategy, two types of organic-silica hybrid monoliths with positively charged quaternary ammonium and amide groups were prepared, respectively. The functional groups were successfully introduced onto the monoliths during the sol-gel process with "thiol-ene" click reaction, which was demonstrated by ζ-potential assessment, energy dispersive X-ray spectroscopy (EDX), and Fourier transform infrared (FT-IR) spectroscopy. The porous structure of the prepared monolithic columns was examined by scanning electron microscopy (SEM), nitrogen adsorption-desorption measurement, and mercury intrusion porosimetry. These results indicate the prepared organic-silica hybrid monoliths possess homogeneous column bed, large specific surface area, good mechanical stability, and excellent permeability. The prepared monolithic columns were then applied for anion-exchange/hydrophilic interaction liquid chromatography. Different types of analytes, including benzoic acids, inorganic ions, nucleosides, and nucleotides, were well separated with high column efficiency around 80,000-130,000 plates/m. Taken together, we present a facile and universal strategy to prepare organic-silica hybrid monoliths with a variety of organic monomers using one-step approach. Copyright © 2013 Elsevier B.V. All rights reserved.

  17. The IS-ENES climate4impact portal: bridging the CMIP5 and CORDEX data to impact users

    Science.gov (United States)

    Som de Cerff, Wim; Plieger, Maarten; Page, Christian; Tatarinova, Natalia; Hutjes, Ronald; de Jong, Fokke; Bärring, Lars; Sjökvist, Elin; Vega Saldarriaga, Manuel; Santiago Cofiño Gonzalez, Antonio

    2015-04-01

    The aim of climate4impact (climate4impact.eu) is to enhance the use of Climate Research Data and to enhance the interaction with climate effect/impact communities. The portal is based on 17 impact use cases from 5 different European countries, and is evaluated by a user panel consisting of use case owners. It has been developed within the IS-ENES European project and is currently operated and further developed in the IS ENES2 project. As the climate impact community is very broad, the focus is mainly on the scientific impact community. Climate4impact is connected to the Earth System Grid Federation (ESGF) nodes containing global climate model data (GCM data) from the fifth phase of the Coupled Model Intercomparison Project (CMIP5) and regional climate model data (RCM) data from the Coordinated Regional Climate Downscaling Experiment (CORDEX). This global network of climate model data centers offers services for data description, discovery and download. The climate4impact portal connects to these services using OpenID, and offers a user interface for searching, visualizing and downloading global climate model data and more. A challenging task is to describe the available model data and how it can be used. The portal informs users about possible caveats when using climate model data. All impact use cases are described in the documentation section, using highlighted keywords pointing to detailed information in the glossary. Climate4impact currently has two main objectives. The first one is to work on a web interface which automatically generates a graphical user interface on WPS endpoints. The WPS calculates climate indices and subset data using OpenClimateGIS/icclim on data stored in ESGF data nodes. Data is then transmitted from ESGF nodes over secured OpenDAP and becomes available in a new, per user, secured OpenDAP server. The results can then be visualized again using ADAGUC WMS. Dedicated wizards for processing of climate indices will be developed in close

  18. First example of stepwise, zwitterionic mechanism for bicyclo[2.2.1]hept-5-ene (norbornene) formation process catalyzed by the 1-butyl-3-methylimidazolium cations.

    Science.gov (United States)

    Jasiński, Radomir

    B3LYP/6-31++G(d) calculations indicated that the reaction of (2 E )-3-phenyl-2-nitroprop-2-enenitrile with cyclopentadiene catalyzed by cations of 1,3-dialkylimidazolium ionic liquid shall not take place according to the classical scheme of one-step [2+4] Diels-Alder cycloaddition. Along the path finally leading to bicyclo[2.2.1]hept-5-ene (norbornene) with a nitro group in endo orientation, the process of bicarbocyclic skeleton formation shall take place according to the domino mechanism, via [2+4] heterocycloadduct. On the other hand, along the path leading finally to bicyclo[2.2.1]hept-5-ene with a nitro group in exo orientation, acyclic adduct with a zwitterionic nature is formed in the first reaction, which undergoes cyclisation next in the second step of the reaction.

  19. Polysiloxane-Based Autonomic Self-Healing Elastomers Obtained through Dynamic Boronic Ester Bonds Prepared by Thiol-Ene "Click" Chemistry.

    Science.gov (United States)

    Zuo, Yujing; Gou, Zhiming; Zhang, Changqiao; Feng, Shengyu

    2016-07-01

    Cross-linked silicone elastomers constructed with dynamic-covalent boronic esters are first synthesized by photoinitiated radical thiol-ene "click" chemistry. The resultant samples can be cut with a sharp knife into two pieces and then healed via the reversibility of the boronic ester cross-linkages to restore the original silicone sample within 30 min. Regulation of luminescent properties is achieved by incorporating organic dye into the elastomers through a "one-pot" thiol-ene reaction. The proposed synthesis procedure demonstrates a new strategy to produce boronic acid silicone materials capable of self-healing without external forces. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  20. [3,3]-Sigmatropic shifts and retro-ene rearrangements in cyanates, isocyanates, thiocyanates, and isothiocyanates of the form RX-YCN and RX-NCY.

    Science.gov (United States)

    Koch, Rainer; Finnerty, Justin J; Murali, Sukumaran; Wentrup, Curt

    2012-02-17

    Retro-ene type [2π + 2π + 2σ] and [3,3]-sigmatropic shift reactions involving the substituent groups R in heteroatom-substituted cyanates and thiocyanates RX-YCN and the isomeric isocyanates and isothiocyanates of the type RX-NCY (X = CR(2), NR', O, or S; Y = O or S) have been investigated computationally at the B3LYP/6-311++G(d,p) level. Retro-ene reactions of alkyl derivatives of the title compounds afford alkenes, imines, carbonyl and thiocarbonyl compounds together with HNCO (HNCS) or HOCN (HSCN). [3,3]-Sigmatropic shifts (hetero-Cope rearrangements) of the corresponding allyl, propargyl, benzyl, and aryl derivatives causes allylic rearrangements, propargyl-allenyl rearrangement, conversion of benzyl cyanates to o-isocyanatotoluenes, and conversion of N-cyanatoarylamines to o-isocyanatoanilines, etc. The corresponding rearrangements of allyl thiocyanates, arylamino thiocyanates and isothiocyanates, and arylsulfenyl thiocyanates and isothiocyanates are also described.

  1. Thiol-ene thermosets exploiting surface reactivity for layer-by-layer structures and control of penetration depth for selective surface reactivity

    DEFF Research Database (Denmark)

    Daugaard, Anders Egede; Westh, Andreas; Pereira Rosinha Grundtvig, Ines

    Thiol-ene thermosets have been shown to be an efficient platform for preparation of functional polymer surfaces. Especially the effectiveness and versatility of the system has enabled a large variety of network properties to be obtained in a simple and straight-forward way. Due to its selectivity......, various thiols and allyl or other vinyl reactants can be used to obtain either soft and flexible1 or more rigid functional thermosets 2. The methodology permits use of etiher thermal or photochemical conditions both for matrix preparation as well as for surface functionalization. Due to excess reactive...... groups in thµe surface of thiol-ene thermosets, it is possible to prepare surface functional thermosets or to exploit the reactive groups for modular construction and subsequent chemical bonding. Here a different approach preparing monolithic layer-by-layer structures with controlled mechanical...

  2. Controlled release of silyl ether camptothecin from thiol-ene click chemistry-functionalized mesoporous silica nanoparticles.

    Science.gov (United States)

    Yan, Yue; Fu, Jie; Wang, Tianfu; Lu, Xiuyang

    2017-03-15

    As efficient drug carriers, stimuli-responsive mesoporous silica nanoparticles are at the forefront of research on drug delivery systems. An acid-responsive system based on silyl ether has been applied to deliver a hybrid prodrug. Thiol-ene click chemistry has been successfully utilized for tethering this prodrug to mesoporous silica nanoparticles. Here, by altering the steric bulk of the substituent on the silicon atom, the release rate of a model drug, camptothecin, was controlled. The synthesized drug delivery system was investigated by analytical methods to confirm the functionalization and conjugation of the mesoporous silica nanoparticles. Herein, trimethyl silyl ether and triethyl silyl ether were selected to regulate the release rate. Under normal plasma conditions (pH 7.4), both types of camptothecin-loaded mesoporous silica nanoparticles (i.e., MSN-Me-CPT and MSN-Et-CPT) did not release the model drug. However, under in vitro acidic conditions (pH 4.0), based on a comparison of the release rates, camptothecin was released from MSN-Me-CPT more rapidly than from MSN-Et-CPT. To determine the biocompatibility of the modified mesoporous silica nanoparticles and the in vivo camptothecin uptake behavior, MTT assays with cancer cells and confocal microscopy observations were conducted, with positive results. These functionalized nanoparticles could be useful in clinical treatments requiring controlled drug release. As the release rate of drug from drug-carrier plays important role in therapy effects, trimethyl silyl ether (TMS) and triethyl silyl ether (TES) were selected as acid-sensitive silanes to control the release rates of model drugs conjugated from MSNs by thiol-ene click chemistry. The kinetic profiles of TMS and TES materials have been studied. At pH 4.0, the release of camptothecin from MSN-Et-CPT occurred after 2h, whereas MSN-Me-CPT showed immediate drug release. The results showed that silyl ether could be used to control release rates of drugs from

  3. Vapor-liquid interfacial reaction to fabricate superhydrophilic and underwater superoleophobic thiol-ene/silica hybrid decorated fabric for oil/water separation

    Science.gov (United States)

    Li, Hongqiang; Liang, Tao; Lai, Xuejun; Su, Xiaojing; Zhang, Lin; Zeng, Xingrong

    2018-01-01

    With oil spill accidents and oil industrial wastewater increasing, oil/water separation has attracted much attention in recent years. Herein, we report the fabrication of superhydrophilic and underwater superoleophobic thiol-ene/silica hybrid decorated fabrics for oil/water separation via vapor-liquid interfacial reaction. It is based on sol-gel reaction of tetraethyl orthosilicate (TEOS) to generate silica and thiol-ene reaction between poly(ethylene glycol) dimethacrylate (PEGDMA) and trimethylolpropane tris(3-mercaptopropionate) (TTMP) to form crosslinked hydrophilic polymer on polyester fabric under the catalysis of butylamine/ammonia vapor. The chemical structure of the surfaces on thiol-ene/silica hybrid decorated fabric was confirmed by FTIR and XPS, and obvious micro-nano morphology and roughness were observed with SEM and AFM. The water contact angle of the fabric attained 0° in 0.36 s, and the underwater oil contact angle reached up to 160°. Importantly, the fabric exhibited high separation efficiency at 99.5%, fast water flux above 71600 Lm-2h-1 and excellent recyclability in oil/water separation. Our findings open a new strategy to fabricate organic-inorganic hybrid superhydrophobic and underwater superoleophobic materials for oil/water separation.

  4. Enantioselective Reduction of Citral Isomers in NCR Ene Reductase: Analysis of an Active-Site Mutant Library.

    Science.gov (United States)

    Kress, Nico; Rapp, Johanna; Hauer, Bernhard

    2017-04-18

    A deeper understanding of the >99 % S-selective reduction of both isomers of citral catalyzed by NCR ene reductase was achieved by active-site mutational studies and docking simulation. Though structurally similar, the E/Z isomers of citral showed a significantly varying selectivity response to introduced mutations. Although it was possible to invert (E)-citral reduction enantioselectivity to ee 46 % (R) by introducing mutation W66A, for (Z)-citral it remained ≥88 % (S) for all single-residue variants. Residue 66 seems to act as a lever for opposite binding modes. This was underlined by a W66A-based double-mutant library that enhanced the (E)-citral derived enantioselectivity to 63 % (R) and significantly lowered the S selectivity for (Z)-citral to 44 % (S). Formation of (R)-citronellal from an (E/Z)-citral mixture is a desire in industrial (-)-menthol synthesis. Our findings pave the way for a rational enzyme engineering solution. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  5. Synthesis and Antimicrobial Activity of N-(6-Carboxyl Cyclohex-3-ene Carbonyl Chitosan with Different Degrees of Substitution

    Directory of Open Access Journals (Sweden)

    Mohamed E. I. Badawy

    2016-01-01

    Full Text Available Five products of N-(6-carboxyl cyclohex-3-ene carbonyl chitosan as antimicrobial agents were prepared by reaction of chitosan with tetrahydrophthalic anhydride (THPA at different degrees of substitution (DS. The antimicrobial activity was evaluated against four plant bacteria and eight fungi. The results proved that the inhibitory property and water solubility of the synthesized chitosan derivatives, with increase of the DS, exhibited a remarkable improvement over chitosan. The product with a DS of 0.40 was the most active one with MIC of 510, 735, 240, and 385 mg/L against Erwinia carotovora, Ralstonia solanacearum, Rhodococcus fascians, and Rhizobium radiobacter, respectively, and also in mycelial growth inhibition against Alternaria alternata (EC50 = 683 mg/L, Botrytis cinerea (EC50 = 774 mg/L, Botryodiplodia theobromae (EC50 = 501 mg/L, Fusarium oxysporum (EC50 = 500 mg/L, F. solani (EC50 = 260 mg/L, Penicillium digitatum (EC50 = 417 mg/L, Phytophthora infestans (EC50 = 298 mg/L, and Sclerotinia sclerotiorum (EC50 = 763 mg/L. These compounds based on a biodegradable and biocompatible chitosan could be used as potentially antimicrobial agents in crop protection instead of hazardous synthetic pesticides.

  6. Olean-18-ene triterpenoids from Celastraceae species inhibit HIV replication targeting NF-kB and Sp1 dependent transcription.

    Science.gov (United States)

    Osorio, Alex A; Muñóz, Alejandro; Torres-Romero, David; Bedoya, Luis M; Perestelo, Nayra R; Jiménez, Ignacio A; Alcamí, José; Bazzocchi, Isabel L

    2012-06-01

    In the present study we report the isolation of nine new olean-18-ene triterpenes (1-9), along with three known ones (10-12), from Cassine xylocarpa and Maytenus jelskii. Their stereostructures have been elucidated on the basis of spectroscopic analysis, including 1D and 2D NMR techniques (COSY, ROESY, HSQC and HMBC), and spectrometric methods. The natural compounds and derivatives 13-15 have been tested for their potential as inhibitors of human immunodeficiency virus type 1 replication. Five compounds from this series displayed potent antiviral activity with IC(50)s in the micromolar range (1, 3, 4, 7 and 8) being 1 and 8 the most active compounds. The target of these compounds was different from antiretroviral drugs currently licensed as they act as inhibitors of enhancer-dependent transcription. The structure-activity relationships were established based on the regiosubstitution and oxidation degree of the triterpene scaffold, revealing that these aspects were able to modulate the selectivity and intensity of HIV inhibition. Copyright © 2012 Elsevier Masson SAS. All rights reserved.

  7. Low-Cost Perovskite Solar Cells Employing Dimethoxydiphenylamine-Substituted Bistricyclic Aromatic Enes as Hole Transport Materials.

    Science.gov (United States)

    Rakstys, Kasparas; Paek, Sanghyun; Grancini, Giulia; Gao, Peng; Jankauskas, Vygintas; Asiri, Abdullah M; Nazeeruddin, Mohammad Khaja

    2017-10-09

    The synthesis, characterization and photovoltaic performance of series of novel molecular hole transport materials (HTMs) based on bistricyclic aromatic enes (BAEs) are presented. The new derivatives were obtained following a simple and straightforward procedure from inexpensive starting reagents mimicking the synthetically challenging 9,9'-spirobifluorene moiety of the well-studied spiro-OMeTAD. The novel HTMs were tested in mixed cations and anions perovskite solar cells (PSCs) yielding a power conversion efficiency (PCE) of 19.2 % under standard global 100 mW cm-2 AM1.5G illumination using 9-{2,7-bis[bis(4-methoxyphenyl)amino]-9H-fluoren-9-ylidene}-N2 ,N2 ,N7 ,N7 -tetrakis(4-methoxyphenyl)-9H-thioxanthene-2,7-diamine (coded as KR374). The power conversion efficiency data confirms the easily attainable heteromerous fluorenylidenethioxanthene structure as valuable core for low-cost and highly efficient HTM design and paves the way towards cost-effective PSC technology. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  8. Thiol-Ene Photo-Click Collagen-PEG Hydrogels: Impact of Water-Soluble Photoinitiators on Cell Viability, Gelation Kinetics and Rheological Properties

    Directory of Open Access Journals (Sweden)

    Róisín Holmes

    2017-06-01

    Full Text Available Thiol-ene photo-click hydrogels were prepared via step-growth polymerisation using thiol-functionalised type-I collagen and 8-arm poly(ethylene glycol norbornene-terminated (PEG-NB, as a potential injectable regenerative device. Type-I collagen was thiol-functionalised by a ring opening reaction with 2-iminothiolane (2IT, whereby up to 80 Abs.% functionalisation and 90 RPN% triple helical preservation were recorded via 2,4,6-Trinitrobenzenesulfonic acid (TNBS colorimetric assay and circular dichroism (CD. Type, i.e., either 2-Hydroxy-1-[4-(2-hydroxyethoxy phenyl]-2-methyl-1-propanone (I2959 or lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP, and concentration of photoinitiator were varied to ensure minimal photoinitiator-induced cytotoxicity and to enable thiol-ene network formation of collagen-PEG mixtures. The viability of G292 cells following 24 h culture in photoinitiator-supplemented media was largely affected by the photoinitiator concentration, with I2959-supplemented media observed to induce higher toxic response (0.1 → 0.5% (w/v I2959, cell survival: 62 → 2 Abs.% compared to LAP-supplemented media (cell survival: 86 → 8 Abs.%. In line with the in vitro study, selected photoinitiator concentrations were used to prepare thiol-ene photo-click hydrogels. Gelation kinetics proved to be largely affected by the specific photoinitiator, with LAP-containing thiol-ene mixtures leading to significantly reduced complete gelation time (τ: 187 s with respect to I2959-containing mixtures (τ: 1683 s. Other than the specific photoinitiator, the photoinitiator concentration was key to adjusting the hydrogel storage modulus (G’, whereby 15-fold G’ increase (232 → 3360 Pa was observed in samples prepared with 0.5% (w/v compared to 0.1% (w/v LAP. Further thiol-ene formulations with 0.5% (w/v LAP and varied content of PEG-NB were tested to prepare photo-click hydrogels with porous architecture, as well as tunable storage modulus (G

  9. Regulation of a putative corticosteroid, 17, 21-dihydroxypregn-4-ene, 3, 20-one, in sea lamprey, Petromyzon marinus.

    Science.gov (United States)

    Roberts, Brent W.; Didier, Wes; Satbir, Rai; Johnson, Nicholas S.; Libants, Scot V.; Sang-Seon, Yun; Close, David

    2013-01-01

    In higher vertebrates, in response to stress, the hypothalamus produces corticotropin-releasing hormone (CRH), which stimulates cells in the anterior pituitary to produce adrenocorticotropic hormone (ACTH), which in turn stimulates production of either cortisol (F) or corticosterone (B) by the adrenal tissues. In lampreys, however, neither of these steroids is present. Instead, it has been proposed that the stress steroid is actually 17,21-dihydroxypregn-4-ene-3,20-dione (11-deoxycortisol; S). However, there have been no studies yet to determine its mechanism of regulation or site of production. Here we demonstrate that (1) intraperitoneal injections of lamprey-CRH increase plasma S in a dose dependent manner, (2) intraperitoneal injections of four lamprey-specific ACTH peptides at 100 lg/kg, did not induce changes in plasma S concentrations in either males or females; (3) two lamprey-specific gonadotropin-releasing hormones (GnRH I and III) and arginine-vasotocin (AVT), all at single doses, stimulated S production as well as, or to an even greater extent than CRH; (4) sea lamprey mesonephric kidneys, in vitro, converted tritiated 17a-hydroxyprogesterone (17a-P) into a steroid that had the same chromatographic properties (on HPLC and TLC) as S; (5) kidney tissues released significantly more immunoassayable S into the incubation medium than gill, liver or gonad tissues. One interpretation of these results is that the corticosteroid production of the sea lamprey, one of the oldest extant vertebrates, is regulated through multiple pathways rather than the classical HPI-axis. However, the responsiveness of this steroid to the GnRH peptides means that a reproductive rather than a stress role for this steroid cannot yet be ruled out.

  10. A stepwise retro-imino-ene as a key step in the mechanism of allene formation via the Crabbé acetylene homologation.

    Science.gov (United States)

    González, Marta; Rodríguez, Roi Álvarez; Cid, Maria Magdalena; López, Carlos Silva

    2012-05-15

    The mechanism of the acetylene homologation procedure accidentally discovered and further developed by Crabbé and coworkers is unknown. Kinetic isotope effect (KIE) experiments, however, suggest that an intramolecular hydrogen shift is the key step of the transformation. In this work, we present a computational study of this mechanism. We found that the reaction proceeds via an unexpected stepwise retro-imino-ene rearrangement. This mechanism justifies the role of Cu(I) as a reaction catalyst and is also compatible with the KIE experiments reported. Copyright © 2012 Wiley Periodicals, Inc.

  11. Facile synthesis of linear-dendritic cholesteryl-poly(epsilon-caprolactone)-b-(L-lysine)(G2) by thiol-ene and azide-alkyne "click" reactions

    DEFF Research Database (Denmark)

    Javakhishvili, Irakli; Binder, W.H.; Tanner, S.

    2010-01-01

    The construction of a linear-dendritic block copolymer consisting of terminal cholesteryl moiety, poly(epsilon-caprolactone), and a second generation L-lysine dendron has been accomplished by the combination of copper(I) catalyzed azide-alkyne and UV-triggered thiol-ene "click" reactions. Ring......-opening polymerization of E-caprolactone initiated by 5-hexyn-1-ol and Mitsunobu coupling with 4-pentenoic acid provide hetero-telechelic poly(epsilon-caprolactone) bearing terminal alkyne and alkene groups. It is then employed in the sequential "click" reactions with the azide-functionalized dendritic wedge...

  12. Volumetric Properties of the Mixture Hex-1-ene C6H12 + C10H12 1,2,3,4-Tetrahydronaphthalene (VMSD1211, LB3980_V)

    Science.gov (United States)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume B 'Binary Liquid Systems of Nonelectrolytes II' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Volumetric Properties of the Mixture Hex-1-ene C6H12 + C10H12 1,2,3,4-Tetrahydronaphthalene (VMSD1211, LB3980_V)' providing data from direct low-pressure dilatometric measurement of molar excess volume at variable mole fraction and constant temperature.

  13. Heat of Mixing and Solution of Hex-1-ene C6H12 + C12H16 Cyclohexylbenzene (HMSD1111, LB4068_H)

    Science.gov (United States)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume B 'Binary Liquid Systems of Nonelectrolytes II' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Heat of Mixing and Solution of Hex-1-ene C6H12 + C12H16 Cyclohexylbenzene (HMSD1111, LB4068_H)' providing data from direct low-pressure calorimetric measurement of molar excess enthalpy at variable mole fraction and constant temperature.

  14. Vapor-Liquid Equilibrium in the Mixture 3-Methylthiophene C5H6S + C6H12 Hex-1-ene (EVLM1131, LB5703_E)

    Science.gov (United States)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume B 'Binary Liquid Systems of Nonelectrolytes II' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'vapor-liquid Equilibrium in the Mixture 3-Methylthiophene C5H6S + C6H12 Hex-1-ene (EVLM1131, LB5703_E)' providing data from direct measurement of pressure and mole fraction in vapor phase at variable mole fraction in liquid phase and constant temperature.

  15. Heat of Mixing and Solution of 2-Methylpyridine C6H7N + C6H12 Hex-1-ene (HMSD1111, LB3864_H)

    Science.gov (United States)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume B 'Binary Liquid Systems of Nonelectrolytes II' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Heat of Mixing and Solution of 2-Methylpyridine C6H7N + C6H12 Hex-1-ene (HMSD1111, LB3864_H)' providing data from direct low-pressure calorimetric measurement of molar excess enthalpy at variable mole fraction and constant temperature.

  16. Volumetric Properties of the Mixture Hex-1-ene C6H12 + C8H10 1,2-Dimethylbenzene (VMSD1212, LB4577_V)

    Science.gov (United States)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume B 'Binary Liquid Systems of Nonelectrolytes II' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Volumetric Properties of the Mixture Hex-1-ene C6H12 + C8H10 1,2-Dimethylbenzene (VMSD1212, LB4577_V)' providing data by calculation of molar excess volume from low-pressure density measurements at variable mole fraction and constant temperature.

  17. Volumetric Properties of the Mixture Hex-1-ene C6H12 + C8H10 1,2-Dimethylbenzene (VMSD1111, LB5004_V)

    Science.gov (United States)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume B 'Binary Liquid Systems of Nonelectrolytes II' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Volumetric Properties of the Mixture Hex-1-ene C6H12 + C8H10 1,2-Dimethylbenzene (VMSD1111, LB5004_V)' providing data from direct low-pressure measurement of mass density at variable mole fraction and constant temperature, in the single-phase region(s).

  18. Volumetric Properties of the Mixture Hex-1-ene C6H12 + C12H16 Cyclohexylbenzene (VMSD1211, LB3987_V)

    Science.gov (United States)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume B 'Binary Liquid Systems of Nonelectrolytes II' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Volumetric Properties of the Mixture Hex-1-ene C6H12 + C12H16 Cyclohexylbenzene (VMSD1211, LB3987_V)' providing data from direct low-pressure dilatometric measurement of molar excess volume at variable mole fraction and constant temperature.

  19. Heat of Mixing and Solution of Hex-1-ene C6H12 + C10H12 1,2,3,4-Tetrahydronaphthalene (HMSD1111, LB4061_H)

    Science.gov (United States)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume B 'Binary Liquid Systems of Nonelectrolytes II' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Heat of Mixing and Solution of Hex-1-ene C6H12 + C10H12 1,2,3,4-Tetrahydronaphthalene (HMSD1111, LB4061_H)' providing data from direct low-pressure calorimetric measurement of molar excess enthalpy at variable mole fraction and constant temperature.

  20. Syntheses of Four Enantiomers of 2,3-Diendo- and 3-Endo-aminobicyclo[2.2.2]oct-5-ene-2-exo-carboxylic Acid and Their Saturated Analogues

    Directory of Open Access Journals (Sweden)

    Márta Palkó

    2013-12-01

    Full Text Available Ethyl 2,3-diendo-3-aminobicyclo[2.2.2]oct-5-ene-2-carboxylate ((±-1 was resolved with O,O'-dibenzoyltartaric acid via diastereomeric salt formation. The efficient synthesis of the enantiomers of 2,3-diendo-3-aminobicyclo[2.2.2]oct-5-ene-2-carboxylic acid ((+-7 and (–-7, 3-endo-aminobicyclo[2.2.2]oct-5-ene-2-exo-carboxylic acid ((+-5 and (–-5, cis- and trans-3-aminobicyclo[2.2.2]octane-2-carboxylic acid ((+-6, (–-6, (+-8 and (–-8 was achieved via isomerization, hydrogenation and hydrolysis of the corresponding esters (–-1 and (+-1. The stereochemistry and relative configurations of the synthesized compounds were determined by NMR spectroscopy (based on the 3J(H,H coupling constants and X-ray crystallography.

  1. Porušena crkva Rođenja Blažene Djevice Marije u Silbi

    Directory of Open Access Journals (Sweden)

    Eugen Motušić

    2014-12-01

    Full Text Available Već otprije je bio poznat podatak da se godine 1828. u Silbi porušila stara crkva Rođenja B.D. Marije kako bi se dobivenim građevinskim materijalom dovršila prekinuta gradnja nove župne crkve koja naslijeđuje i njen titular. Prema arhivskim podacima rušenje je dozvolio zadarski nadbiskup Josip Nowak i odredio da se do izgradnje nove crkve kao župna koristi nekadašnja franjevačka Gospe od Karmela. Od čitavog je sklopa do danas sačuvan tek zvonik koji je ostao služiti novoj župnoj crkvi. Prema shematiziranim tlocrtu i dimenzijama porušene crkve, dokumentiranima u danas zagubljenom spisu iz župnog arhiva, razvidno je da se radilo o jednobrodnoj longitudinalnoj crkvi s pravokutnom, od lađe užom sakristijom na začelju, dvjema plitkim kapelama dograđenima uz pobočne zidove, te otvorenim predvorjem (ložom duž pročelja koje se svojim južnim uglom oslanjalo na ugao zvonika. Crkvena je dvorana osim glavnog oltara prislonjenog uz začelje lađe imala tri para pobočnih oltara. Analiza apostolskih i kanonskih vizitacija crkve, prvi puta spomenute 1579. godine, pokazuje da je još u drugoj četvrtini 17. stoljeća ona bila skromna građevina u kojoj se, radi udaljenosti tadašnje župne crkve sv. Marka od naselja, čuva ulje za bolesničko pomazanje. Ipak, crkva je tada imala pet pobočnih oltara koje podižu ugledniji pojedinci i laičke bratovštine od kojih se isticala ona Gospe od Ružarija po kojoj se i crkva tijekom 18. stoljeća pučki nazivala. Na temelju analize zapisa vizitacije nadbiskupa Evanđeliste Parzaghija iz 1670. godine, zaključuje se da je crkva netom prije njegova pohoda pregrađena, dok se prilikom opisa pojedinih oltara navodi da su u ovoj obnovi mijenjali poziciju. Godine 1678. prvi se put spominje zvonik – kampanel. Komparativnom analizom može se ustvrditi da je crkva Male Gospe u Silbi svojom tipologijom pripadala brojnoj skupini jednostavnih, prostranih, ali prema građevnim oblicima ruralnih crkava

  2. Metabolism of selective 20-epi-vitamin D3 analogs in rat osteosarcoma UMR-106 cells: Isolation and identification of four novel C-1 fatty acid esters of 1α,25-dihydroxy-16-ene-20-epi-vitamin D3.

    Science.gov (United States)

    Flarakos, Caroline Ceailles; Weiskopf, Andrew; Robinson, Matthew; Wang, Guoshun; Vouros, Paul; Sasso, Gino J; Uskokovic, Milan R; Reddy, G Satyanarayana

    2017-03-01

    Analogs of 1α,25-dihydroxyvitamin D3 (S1) with 20-epi modification (20-epi analogs) possess unique biological properties. We previously reported that 1α,25-dihydroxy-20-epi-vitamin D3 (S2), the basic 20-epi analog is metabolized into less polar metabolites (LPMs) in rat osteosarcoma cells (UMR-106) but not in a perfused rat kidney. Furthermore, we also noted that only selective 20-epi analogs are metabolized into LPMs. For example, 1α,25-dihydroxy-16-ene-20-epi-vitamin D3 (S4), but not 1α,25-dihydroxy-16-ene-23-yne-20-epi-vitamin D3 (S5) is metabolized into LPMs. In spite of these novel findings, the unequivocal identification of LPMs has not been achieved to date. We report here on a thorough investigation of the metabolism of S4 in UMR-106 cells and isolated two major LPMs produced directly from the substrate S4 itself and two minor LPMs produced from 3-epi-S4, a metabolite of S4 produced through C-3 epimerization pathway. Using GC/MS, ESI-MS and (1)H NMR analysis, we identified all the four LPMs of S4 as 25-hydroxy-16-ene-20-epi-vitamin D3-1-stearate and 25-hydroxy-16-ene-20-epi-vitamin D3-1-oleate and their respective C-3 epimers. We report here for the first time the elucidation of a novel pathway of metabolism in UMR-106 cells in which both 1α,25(OH)2-16-ene-20-epi-D3 and 1α,25(OH)2-16-ene-20-epi-3-epi-D3 undergo C-1 esterification into stearic and oleic acid esters. Copyright © 2017 Elsevier Inc. All rights reserved.

  3. Ära söö kiles saia ehk kas poliitilist kunsti on võimalik mõista? = Do not eat white bread in plastic, or is it possible to understand political art? / Ene-Liis Semper, Eero Epner

    Index Scriptorium Estoniae

    Semper, Ene-Liis, 1969-

    2006-01-01

    Poliitillisest teatrist. NO99 lavastusest "Nafta" (lavastajad, autorid: Tiit Ojasoo, Ene-Liis Semper, kunstnik Ene-Liis Semper). Ettekanne Kaasaegse Kunsti Eesti Keskuse 4. ettekannete päeval "Kunstnike moraalsed valikud kultuurikonfliktides" Tallinna Kunstihoones 27.04.2006. Bibliograafia lk. 112

  4. Assessment of the role of in situ generated (E)-2,4-diene-valproic acid in the toxicity of valproic acid and (E)-2-ene-valproic acid in sandwich-cultured rat hepatocytes

    Energy Technology Data Exchange (ETDEWEB)

    Surendradoss, Jayakumar; Chang, Thomas K.H.; Abbott, Frank S., E-mail: frank.abbott@ubc.ca

    2012-11-01

    Valproic acid (VPA) undergoes cytochrome P450-mediated desaturation to form 4-ene-VPA, which subsequently yields (E)-2,4-diene-VPA by β-oxidation. Another biotransformation pathway involves β-oxidation of VPA to form (E)-2-ene-VPA, which also generates (E)-2,4-diene-VPA by cytochrome P450-mediated desaturation. Although the synthetic form of (E)-2,4-diene-VPA is more hepatotoxic than VPA as shown in various experimental models, there is no conclusive evidence to implicate the in situ generated (E)-2,4-diene-VPA in VPA hepatotoxicity. The present study investigated the effects of modulating the in situ formation of (E)-2,4-diene-VPA on markers of oxidative stress (formation of 2′,7′-dichlorofluorescein; DCF), steatosis (accumulation of BODIPY 558/568 C{sub 12}), necrosis (release of lactate dehydrogenase; LDH), and on cellular total glutathione (GSH) levels in sandwich-cultured rat hepatocytes treated with VPA or (E)-2-ene-VPA. Treatment with either of these chemicals alone increased each of the toxicity endpoints. In VPA-treated hepatocytes, (E)-2,4-diene-VPA was detected only at trace levels, even after phenobarbital (PB) pretreatment and there was no effect on the toxicity of VPA. Furthermore, pretreatment with a cytochrome P450 enzyme inhibitor, 1-aminobenzotriazole (1-ABT), did not influence the extent of VPA toxicity in both PB-pretreated and vehicle-pretreated hepatocytes. However, in (E)-2-ene-VPA-treated hepatocytes, PB pretreatment greatly enhanced the levels of (E)-2,4-diene-VPA and this was accompanied by a further enhancement of the effects of (E)-2-ene-VPA on DCF formation, BODIPY accumulation, LDH release, and GSH depletion. Pretreatment with 1-ABT reduced the concentrations of (E)-2,4-diene-VPA and the extent of (E)-2-ene-VPA toxicity; however, this occurred in PB-pretreated hepatocytes, but not in control hepatocytes. In conclusion, in situ generated (E)-2,4-diene-VPA is not responsible for the hepatocyte toxicity of VPA, whereas it

  5. Novel Anti-Biofouling Soft Contact Lens: l-Cysteine Conjugated Amphiphilic Conetworks via RAFT and Thiol-Ene Click Chemistry.

    Science.gov (United States)

    Zhang, Chengfeng; Liu, Ziyuan; Wang, Haiye; Feng, Xiaofeng; He, Chunju

    2017-07-01

    A unique l-cysteine conjugated antifouling amphiphilic conetwork (APCN) is synthesized through end-crosslinking of well-defined triblock copolymers poly(allyl methacrylate)-b-poly(ethylene glycol)-b-poly(allyl methacrylate) via a combination of reversible addition-fragmentation chain transfer (RAFT) polymerization and thiol-ene "click" chemistry. The synthesized poly(ethylene glycol) macro-RAFT agent initiates the polymerization of allyl methacrylate in a controlled manner. The vinyl pendant groups of the precursor partially conjugate with l-cysteine and the rest fully crosslink with mercaptopropyl-containing siloxane via thiol-ene click chemistry under UV irradiation into APCNs, which show distinguished properties, that is, excellent biocompatibility, more than 39.6% water content, 101 barrers oxygen permeability, optimized mechanical properties, and more than 93% visible light transmittance. What's more, the resultant APCNs exhibit eminent resistance to protein adsorption, where the bovine serum albumin and lysozyme adsorption are decreased to 12 and 21 µg cm(-2) , respectively. The outstanding properties of APCNs depend on the RAFT controlled method, which precisely designs the hydrophilic/hydrophobic segments and eventually greatly improves the crosslinking efficiency and homogeneity. Meantime, the l-cysteine monolayer can effectively reduce the surface hydrophobicity and prevent protein adsorption, which exhibits the viability for antifouling surface over and under ophthalmic devices, suggesting a promising soft contact lens. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  6. Facile one-pot preparation of a novel imidazolium-based monolith by thiol-ene click chemistry for capillary liquid chromatography.

    Science.gov (United States)

    Zhang, Peng; Yang, Haiguan; Chen, Tao; Qin, Yuemei; Ye, Fanggui

    2017-11-01

    In this work, a novel imidazolium-based monolith was fabricated through a simple route. With 1-vinyl-3-octadecylimidazolium bromide and ethylene dimethacrylate as monomers, pentaerythritol tetra-(3-mercaptopropionate) as crosslinker, AIBN as thermal initiator, the monolith was facilely fabricated by one-pot thiol-ene click chemistry. The influences of both the content of monomer/crosslinker and porogenic systems on the morphology, and permeability of the monolith were studied. The optimal reaction conditions were used to prepare a homogeneous and permeable monolith. The optimal preparation of monolithic column was characterized by scanning electron microscopy, Fourier transform infrared spectrometry, X-ray photoelectron spectroscopy, and thermogravimetric analysis. High efficiency and quick separation of alkylbenzenes, styrene and ethylbenzene, polycyclic aromatic hydrocarbon, phenols, anilines, and aromatic acids were achieved. The minimum plate height of this monolith were determined as 11.42 μm for thiourea and 13.26 μm for benzene. All results indicated that thiol-ene click chemistry provides a quick way for the fabrication of imidazolium-based monolith. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  7. Pyriculins A and B, two monosubstituted hex-4-ene-2,3-diols and other phytotoxic metabolites produced by Pyricularia grisea isolated from buffelgrass (Cenchrus ciliaris).

    Science.gov (United States)

    Masi, Marco; Meyer, Susan; Górecki, Marcin; Mandoli, Alessandro; Di Bari, Lorenzo; Pescitelli, Gennaro; Cimmino, Alessio; Cristofaro, Massimo; Clement, Suzette; Evidente, Antonio

    2017-11-01

    Pyricularia grisea has been identified as a foliar pathogen on buffelgrass (Cenchrus ciliaris) in North America and was studied as a potential source of phytotoxins for buffelgrass control. Two monosubstituted hex-4-ene-2,3-diols, named pyriculins A and B, were isolated from its culture filtrate organic extract together with (10S,11S)-(-)-epipyriculol, trans-3,4-dihydro-3,4,8-trihydroxy-1(2H)-napthalenone, and (4S)-(+)-isosclerone. Pyriculins A and B were characterized by spectroscopic (essentially nuclear magnetic resonance [NMR], High-resolution electrospray ionization mass spectrometry [HRESIMS]) and chemical methods such as (4E)-1-(4-hydroxy-1,3-dihydroisobenzofuran-1-yl)hex-4-ene-2,3-diols. The relative and absolute configuration of these compounds was determined by a combination of spectroscopic (NMR, electronic circular dichroism [ECD]) and computational tools. When bioassayed in a buffelgrass coleoptile and radicle elongation test, (10S,11S)-(-)-epipyriculol proved to be the most toxic compound. Seed germination was much reduced and slowed with respect to the control and radicles failed to elongate. All five compounds delayed germination, but only (10S,11S)-(-)-epipyriculol was able to prevent radicle development of buffelgrass seedlings. It had no effect on coleoptile elongation, while the other four compounds caused significantly increased coleoptile development relative to the control. © 2017 Wiley Periodicals, Inc.

  8. A combination of "thiol-ene" click chemistry and surface initiated atom transfer radical polymerization: Fabrication of boronic acid functionalized magnetic graphene oxide composite for enrichment of glycoproteins.

    Science.gov (United States)

    Su, Jie; He, Xiwen; Chen, Langxing; Zhang, Yukui

    2018-04-01

    An efficient glycoproteins enrichment platform is one of vital preprocessing steps in biomarker research and in particular glycoproteomics. In this work, a well-defined boronic acid functionalized magnetic graphene oxide nanocomposite (Fe3O4-GO@PAAPBA) was synthesized for the selective enrichment of glycoproteins from complex biological samples via a novel strategy based on the "thiol-ene" click chemistry and surface initiated atom transfer radical polymerization (SI-ATRP). The initiator of ATRP was anchored to the surface of substrate through "thiol-ene" click reaction. The product Fe3O4-GO@PAAPBA was successfully synthesized in following SI-ATRP. The Fe3O4-GO@PAAPBA nanocomposite was characterized by transmission electron microscopy (TEM), Fourier transform-infrared spectroscopy (FTIR), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), vibrating sample magnetometry (VSM) and thermogravimetric analysis. The adsorption capacity of Fe3O4-GO@PAAPBA towards ovalbumin (OVA) and transferrin (Trf) is 471mgg-1 and 450mgg-1, respectively. The nanocomposite also featured good selectivity to glycoproteins in the mixture of glycoproteins and non-glycoproteins at alkaline (pH 9.0) and physiological conditions (pH 7.4). Furthermore, it can be applied to extract glycoproteins directly from egg white samples. These results have indicated that Fe3O4-GO@PAAPBA was a potential affinity material in glycoprotein analysis. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. Optimization of the surface modification process of cross-linked polythiol-coated chiral stationary phases synthesized by a two-step thiol-ene click reaction.

    Science.gov (United States)

    Schmitt, Kristina; Lämmerhofer, Michael

    2018-01-11

    A new platform technology for the preparation of stable chiral stationary phases was successfully optimized. The chiral selector tert-butylcarbamoylquinine was firstly covalently connected to the polymer poly(3-mercaptopropyl)methylsiloxane by thiol-ene click reaction. Secondly, the quinine carbamate functionalized polysiloxane conjugate was coated onto the surface of vinyl modified silica particles and cross-linked via thiol-ene click reaction. The amount of polysiloxane, chiral selector, radical initiator, reaction solvent (chloroform and methanol), reaction time, and pore size of the supporting silica particles were varied and systematically optimized in terms of achievable plate numbers while maintaining simultaneously enantioselectivity. The optimization was based on elemental analysis data, chromatographic results, and H/u-curves (Van Deemter) of the resultant chiral stationary phases. The results suggest that better chromatographic efficiency (higher plate numbers) at equal enantioselectivity can be achieved with methanol (a poor solvent for the polysiloxane that is dispersed rather than dissolved) and a lower film thickness of quinine carbamate functionalized polysiloxane. In this study, chiral stationary phases based on 100 Å silica slightly outperformed 200 Å silica particles (each 5 μm). The optimized two step material exhibited significantly reduced mass transfer resistance compared to the one step material and equal performance as a brush-type chiral stationary phase. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  10. Mida arutati Brüsselis / Ülo Kalm

    Index Scriptorium Estoniae

    Kalm, Ülo, 1935-

    2004-01-01

    EL Põllumajandusorganisatsioonide Komitee (COPA) ja Põllumajanduskoostöö Keskkomitee (COGEGA) piimatöögrupi, COPA mahekomisjoni ning Euroopa Komisjoni nõuandva komitee töögrupi istungitel arutluse all olnud teemadest

  11. DOCOMOMO Eestis : kas mõikab? / Mart Kalm

    Index Scriptorium Estoniae

    Kalm, Mart, 1961-

    1996-01-01

    DOCOMOMO eesti töörühm on tegutsenud üle kolme aasta. Funktsionalismi väärtustamisest Eestis, moodsa arhitektuuri saatusest. H. Johansoni villast Suur-Ameerika 12, selle 1996. a. ümber- ja juurdeehitusest. Pärnu rannakohvikust ja rannahotellist. Kunstihoone restaureerimisest. E. Lohu Rahumäe kabeli ümberehitusest,. O. Siinmaa Pärnu kõlakoja rekonstruktsioonist. P. Tarvase "Kalevi" jahtklubi sülesehitamisest. Mahajäetult seisavad A. Volbergi Eesti Punase Risti sanatoorium Haapsalus, R. Natuse Valdeku saun Nõmmel.

  12. Leila Pärtelpoeg and the Soviet antique / Mart Kalm

    Index Scriptorium Estoniae

    Kalm, Mart, 1961-

    2010-01-01

    Sisearhitekt Leila Pärtelpojast, kes 2010. a. pälvis EV kultuuri elutööpreemia. Nõukogude ajal tegeles antiikmööbliga, uuris mööbliajalugu, kogus ajaloolist mööblit. Sisearhitekti tööst Eesti NSV-s, võimalusest kavandada mööblit. L. Pärtelpoja rollist ajalooliste interjööride (Parlamendihoone Toompeal, mõisahooned) restaureerimisel ja sisustamisel. Tallinna raekoja restaureerimisest (1975), ajalugu ja kaasaega sünteesivast terviklikust kujundusest

  13. Eesti funk vene pähe / Mart Kalm

    Index Scriptorium Estoniae

    Kalm, Mart, 1961-

    1996-01-01

    Raamat "Functional Architecture. The International Style. Funktionale Architektur. Le Style International. 1925- 1940" Saksa kirjastuselt Benedikt Taschen aastast 1990. Raamatus võib Sowjetunioni all lk. 322 leida foto A. Nürnbergi projekteeritud Pärnu hotellist "Vasa" (1938, praegu sanatoorium "Sõprus").

  14. Maailmapärand kisub kiiva / Mart Kalm

    Index Scriptorium Estoniae

    Kalm, Mart, 1961-

    2011-01-01

    19.-29. juunil Pariisis toimunud UNESCO maailmapärandi komitee 35. istungist, kus Eesti osales liikmena. Maailmapärandi nimekirja tehti 25 uut kannet. Maailmapärandi konventsiooni põhimõtete järgimisest

  15. The Pleasures of Mud : Estonian Summer Resort Architecture / Mart Kalm

    Index Scriptorium Estoniae

    Kalm, Mart, 1961-

    2006-01-01

    Pärnu, Haapsalu, Kuressaare ja Narva-Jõesuu suvitusarhitektuurist 20. saj. esimesest poolest kuni 21. saj. alguseni. Arhitektide Olev Siinmaa, Anton Soansi, August Volbergi, Tõnis Mihkelsoni, Raivo Puusepa, Raivo Kotovi, Üla Koppeli, Andrus Kõresaare, ehitusinseneride Erich Ottingi ja Robert Ederma ning sisearhitektide Tüüne-Kristin Vaikla, Urmo Vaikla, Liisi Murula ja Raili Palingu loomingust

  16. Eesti Panga ehitised 1918-1940 / Mart Kalm

    Index Scriptorium Estoniae

    Kalm, Mart, 1961-

    2004-01-01

    Sõdadevaheline Eesti ja pangaehitus. Eesti Pank ehitab: Viljandi osakonna hoone (1927, K. Burman, taastati 1956-61 I. Jaaguse projekti järgi), Petseri osakonna hoone (1929-30, F. Adoff), Rakvere osakonna hoone (1933, F. Adoff), Tallinna peapanga hoone arhitektuurivõistlus (1933, I auhind - E. Sacharias, B. Tshernov) ja peapanga hoone (1933-35, F. Adoff, H. Johanson, E. Habermann), Tartu osakonna hoone (1935-36, A. Matteus, K. Burman, aiakujundaja J. Port) ja selle skulptuurkaunistused (J. Raudsepp, A. Eller), Võru osakonna hoone (1937-38, E. Kuusik, A. Soans, reljeefid - A. Kaasik), Pärnu pangahoone arhitektuurivõistlus (1939, parimad A. Kotli ja E. Kuusik-A. Soans) ja Pärnu osakonna hoone (1939-u. 1945, A. Kotli, A. Soans, reljeefid - J. Raudsepp), ametnike elamu Tallinnas Kentmanni 20A (1924-25), Eesti Maakrediitseltsi büroo- ja eluhoone Tallinnas Sakala 4 (1935-36, E. Kuusik)

  17. Kultuskirjanik Pratchett / Ene Paaver

    Index Scriptorium Estoniae

    Paaver, Ene, 1963-

    2001-01-01

    Terry Pratchetti "Mort - Surma õpipoiss" Eesti Draamateatris, lavastaja Ain Prosa. Pratchetti kohta avaldavad arvamust lavakujundaja Maret Kukkur, dramatiseerija Urmas Vadi ja laulusõnade autor Villu Kangur. Esietendus 7. juunil

  18. Whats 'App-ening'

    DEFF Research Database (Denmark)

    Ankersen, Dorit V; Weimers, Petra; Burisch, Johan

    2017-01-01

    in number. ehealth helps patients in coping with their disease by promoting self-management, which increases adherence to medical treatment and diets, and leads to an improved quality of life. For irritable bowel syndrome (IBS), there are multiple applications that provide dietary advice, for example, a low...

  19. Grunge kodukujunduses / Ene Vool

    Index Scriptorium Estoniae

    Vool, Ene, 1948-

    2004-01-01

    Low-tech, campi ja grunge ehitus- ja sisustusstiilidest meil ja mujal - kommenteerivad Andro Kööp ja Ain Nurmela. Tutvustatakse ka Mari-Mira projekti Pariisis, mis kujutab endast nn. mutant-küla, putka- ja onnikultuuri koketeriid. Artikli lõpus kommenteerib kunstiteadlane Karin Paulus grungestiili muusikas, riietuses ja sisekujunduses.

  20. Ene-Obong, HN

    African Journals Online (AJOL)

    based Oral Rehydration Solution for Home Management of Diarrhoea in Children Abstract · Vol 33, No 1 (2012) - Articles Dietary Habits and Nutritional Status of Rural School Age Children in Ebonyi State, Nigeria Abstract. ISSN: 0189-0913.

  1. Taevo Gans / Ene Ammer

    Index Scriptorium Estoniae

    Ammer, Ene

    1998-01-01

    Sisearhitekt Taevo Gansist. Tudengipõlvest, selle aja projektidest, sõpruskonnast, tandemist Summatavet & Gans, Venemaa tellimustest, kaastöölistest. Üksinda Hommilkumaal vene tarbekunsti näitusega 1974. a. 1988. a. loodud perefirmast "GaDis" (omanikud Taevo, Helle Gans, Riia Oja), mis nõustab ka "Wermot" mööbli osas. "GaDise" sisekujundusprojektidest, millega Taevo ja Helle Gans tegelevad üheskoos

  2. Limitations of the Neurological Evolutional Exam (ENE as a motor assessment for first graders Limitações do Exame Neurológico Evolutivo (ENE como um instrumento de avaliação motora para crianças da primeira série

    Directory of Open Access Journals (Sweden)

    Priscila M. Caçola

    2010-10-01

    Full Text Available BACKGROUND: Many clinicians and researchers in Brazil consider the Neurological Developmental Exam (NDE, a valid and reliable assessment for Brazilian school-aged children. However, since its inception, several tests have emerged that, according to some researchers, provide more in-depth evaluation of motor ability and go beyond the detection of general motor status (soft neurological signs. OBJECTIVES: To highlight the limitations of the NDE as a motor skill assessment for first graders. METHODS: Thirty-five children were compared on seven selected items of the NDE, seven of the Bruininks-Oseretsky Test (BOT, and seven of the Visual-Motor Integration test (VMI. Participants received a "pass" or "fail" score for each item, as prescribed by the respective test manual. RESULTS: Chi-square and ANOVA results indicated that the vast majority of children (74% passed the NDE items, whereas values for the other tests were 29% (BOT and 20% (VMI. Analysis of specific categories (e.g. visual, fine, and gross motor coordination revealed a similar outcome. CONCLUSIONS: Our data suggest that while the NDE may be a valid and reliable test for the detection of general motor status, its use as a diagnostic/remedial tool for identifying motor ability is questionable. One of our recommendations is the consideration of a revised NDE in light of the current needs of clinicians and researchers.CONTEXTUALIZAÇÃO: Muitos clínicos e pesquisadores brasileiros consideram o Exame Neurológico Evolutivo (ENE, um instrumento válido e confiável para crianças brasileiras em idade escolar. Entretanto, desde a sua criação, surgiram outros testes para uma avaliação mais profunda de habilidade motora, os quais vão além de detectar status motor geral em forma de sinais neurológicos leves. OBJETIVOS: Demonstrar os pontos fracos do ENE como teste de avaliação de habilidade motora para crianças de primeira série. MÉTODOS: Trinta e cinco crianças realizaram 7 itens

  3. Poly[[(μ3-5,6-dicarboxybicyclo[2.2.2]oct-7-ene-2,3-dicarboxylato(1,10-phenanthrolinecopper(II] monohydrate

    Directory of Open Access Journals (Sweden)

    Ji-Cheng Ma

    2008-12-01

    Full Text Available In the title compound, {[Cu(C12H10O8(C12H8N2]·H2O}n, the CuII ion is five-coordinated by two N atoms from one phenanthroline ligand and three O atoms from three different H2L2− anions (H4L is bicyclo[2.2.2]oct-7-ene-2,3,5,6-tetracarboxylic acid in a distorted square-pyramidal geometry. Each H2L2− ion bridges three CuII atoms to form a zigzag sheet parallel to the ab plane. The crystal structure is consolidated by O—H...O hydrogen bonds.

  4. Çene Eklemi Rahatsızlıklarında Teşhis ve Tedavi Yöntemleri (*)

    OpenAIRE

    YENGİN, Esengün

    2013-01-01

    ÖZETÇene ekleminin fonksiyon bozukluklarında romatoid artrit, osteoartrit ve travmatik artritlerin yanı sıra ruhi bunalımlar, akut eklem romatizması, neoplazmalar ve enfeksiyöz artrit gibi hastalıklar etkili olabilmektedir. Bu hastalıkların ayırıcı teşhisinde klinik muayenenin yanı sıra, TME radyografileri, çiğneme kaslarının elektromiyografik tetkiki ile klinik ve serolojik testler yapmakta fayda vardır.Hastalarda genellikle TME tıkırtısı ve ağrısı, masseler ve dış pterigoid kaslarda ağrı, k...

  5. C(10)-C(19) bond cleavage reaction of 19-oxygenated androst-4-ene-3,6-dione steroids under various conditions.

    Science.gov (United States)

    Nagaoka, Masao; Numazawa, Mitsuteru

    2004-08-01

    C(10)-C(19) bond cleavage reaction of 19-hydroxy- and 19-oxoandrost-4-ene-3,6,17-triones (5, 6) was explored under various conditions. Treatment of steroids 5 and 6 with KOH in MeOH gave the A-ring aromatized product 6-oxoestrone (11) in a fair yield, respectively, in contrast, the treatment with a weak base yielded 4-methyl steroid 17 (20%) in the case of 19-alcohol 5 or 19-nor-Delta(5(10))-steroid 9 (12-67%) along with compound 11 (6-27%) in the case of 19-aldehyde 6. Reaction of compound 6 with HCl in MeOH produced 3-methyl ethers of 6-oxoestrone and Delta(6)-estrone, compounds 12 and 14 (ca. 20% each). Thus, 6-oxosteroids 5 and 6 showed unique C(10)-C(19) bond cleavage reactions with a base or acid.

  6. A Rapid One-Pot Synthesis of Novel High-Purity Methacrylic Phosphonic Acid (PA-Based Polyhedral Oligomeric Silsesquioxane (POSS Frameworks via Thiol-Ene Click Reaction

    Directory of Open Access Journals (Sweden)

    K. Karuppasamy

    2017-05-01

    Full Text Available Herein, we demonstrate a facile methodology to synthesis a novel methacrylic phosphonic acid (PA-functionalized polyhedral oligomeric silsesquioxanes (POSSs via thiol-ene click reaction using octamercapto thiol-POSS and ethylene glycol methacrylate phosphate (EGMP monomer. The presence of phosphonic acid moieties and POSS-cage structure in POSS-S-PA was confirmed by Fourier transform infrared (FT-IR and nuclear magnetic resonance (1H, 29Si and 31P-NMR analyses. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF mass spectrum of POSS-S-PA acquired in a dithranol matrix, which has specifically designed for intractable polymeric materials. The observed characterization results signposted that novel organo-inorganic hybrid POSS-S-PA would be an efficacious material for fuel cells as a proton exchange membrane and high-temperature applications due to its thermal stability of 380 °C.

  7. The Molecular Structure and Vibrational Spectrum of 6-bromo-8-thia-1,4-epoxybicyclo[4.3.0]non-2-ene

    Directory of Open Access Journals (Sweden)

    Aydın Demircan

    2007-10-01

    Full Text Available Geometric parameters and FT-IR spectrum of 6-bromo-8-thia-1,4-epoxybicyclo[4.3.0]non-2-ene were computed by the HF, B3LYP, B3PW91 andmPW1PW91 methods in conjunction with the 6-31G(d,p basis set. The calculated IRspectra are in a good agreement with the observed FT-IR spectrum. A general betterperformance of B3LYP, B3PW91 and mPW1PW91 versus HF was quantitativelycharacterized by using PAVF 1.0 program. Optimal uniform scaling factors calculated forthe title compound are 0.8952, 0.9552, 0.9520 and 0.9456 for HF, B3LYP, B3PW91 andMPW1PW91 methods, respectively.

  8. One-pot synthesis of (3-sulfopropyl methacrylate potassium)-silica hybrid monolith via thiol-ene click chemistry for CEC.

    Science.gov (United States)

    Yang, Huihui; Chen, Yingzhuang; Liu, Yixuan; Nie, Lihua; Yao, Shouzhuo

    2013-02-01

    A novel (3-sulfopropyl methacrylate potassium)-silica hybrid monolithic column for CEC has been prepared by a simple one-pot approach based on efficient thiol-ene click chemistry. In this process, the polycondensation of hydrolyzed alkoxysilanes and in situ click reaction of vinyl groups on 3-sulfopropyl methacrylate potassium and thiol groups on the precondensed siloxanes simultaneously occurred in a pretreated capillary. Homogeneous monolithic matrix with large through-pores tightly bonded to the inner wall of the capillary was shown by optical microscope and SEM. The minimum plate height of this hybrid monolithic column was determined as 3.9 μm for thiourea. Anilines, alkylbenzenes, and phenols were well separated on this hybrid monolithic column by CEC, which indicated typical reversed-phase and cation-exchange chromatographic retention mechanisms of the column. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  9. Preparation of polyhedral oligomeric silsesquioxane-based hybrid monolith by ring-opening polymerization and post-functionalization via thiol-ene click reaction.

    Science.gov (United States)

    Liu, Zhongshan; Ou, Junjie; Lin, Hui; Wang, Hongwei; Dong, Jing; Zou, Hanfa

    2014-05-16

    A polyhedral oligomeric silsesquioxane (POSS) hybrid monolith was simply prepared by using octaglycidyldimethylsilyl POSS (POSS-epoxy) and cystamine dihydrochloride as monomers via ring-opening polymerization. The effects of composition of prepolymerization solution and polycondensation temperature on the morphology and permeability of monolithic column were investigated in detail. The obtained POSS hybrid monolithic column showed 3D skeleton morphology and exhibited high column efficiency of ∼71,000 plates per meter in reversed-phase mechanism. Owing to this POSS hybrid monolith essentially possessing a great number of disulfide bonds, the monolith surface would expose thiol groups after reduction with dithiothreitol (DTT), which supplied active sites to functionalize with various alkene monomers via thiol-ene click reaction. The results indicated that the reduction with DTT could not destroy the 3D skeleton of hybrid monolith. Both stearyl methylacrylate (SMA) and benzyl methacrylate (BMA) were selected to functionalize the hybrid monolithic columns for reversed-phase liquid chromatography (RPLC), while [2-(methacryloyloxy)ethyl]-dimethyl-(3-sulfopropyl)-ammonium hydroxide (MSA) was used to modify the hybrid monolithic column in hydrophilic interaction chromatography (HILIC). These modified hybrid monolithic columns could be successfully applied for separation of small molecules with high efficiency. It is demonstrated that thiol-ene click reaction supplies a facile way to introduce various functional groups to the hybrid monolith possessing thiol groups. Furthermore, due to good permeability of the resulting hybrid monoliths, we also prepared long hybrid monolithic columns in narrow-bore capillaries. The highest column efficiency reached to ∼70,000 plates using a 1-m-long column of 75μm i.d. with a peak capacity of 147 for isocratic chromatography, indicating potential application in separation and analysis of complex biosamples. Copyright © 2014

  10. Liquid chromatography–tandem mass spectrometry method for simultaneous determination of valproic acid and its ene-metabolites in epilepsy patient plasma

    Directory of Open Access Journals (Sweden)

    Huan Lu

    2016-04-01

    Full Text Available A simple and high throughput method was developed and validated for simultaneous determination of valproic acid and its two toxicant ene-metabolites, 2-enevalproic acid and 4-enevalproic acid in epilepsy patient plasma using liquid chromatography–tandem mass spectrometry. Probenecid was used as internal standard and solid-phase extraction was selected for sample preparation. A chromatographic separation was performed on an Agilent Poroshell SB-C18 column (50 mm×4.6 mm i.d., 2.7 μm by an optimized gradient elution at a flow rate of 0.9 mL/min. The total run time was 7 min. Electrospray ionization was used in negative ion mode by multiple reaction monitoring of the precursor-to-product ion transitions at m/z 143.0→143.0 for valproic acid, m/z 140.9→140.9 for 2-enevalproic acid and 4-enevalproic acid for their poor fragments, and m/z 283.9→239.9 for probenecid. The results showed good linearity of valproic acid, 2-enevalproic acid and 4-enevalproic acid in their respective linear ranges. The correlation coefficients were more than 0.998. The intra- and inter-day precision of the assay was less than 11.0% and the accuracy ranged from 2% to 12%. This analytical method was successfully applied to assay plasma concentrations of valproic acid and its two ene-metabolites in epilepsy patient plasma and used for therapeutic drug monitoring.

  11. Eksperimentalno ispitivanje aerodinamičkih karakteristika modela laserski vođene bombe / Experimental investigation of aerodynamic characteristics of the laser guided bomb model

    Directory of Open Access Journals (Sweden)

    Dijana Damljanović

    2005-09-01

    Full Text Available U radu su prikazani rezultati merenja aerodinamičkih sila i momenata, kao i vizualizacija strujanja u graničnom sloju na krilima i krmilima modela laserski vođene bombe. Ispitivanje je izvršeno u aerotunelu T-38. Analiziranje uticaj rastojanja vrha konusa tela, u odnosu na sekciju krmila, na aerodinamičke karakteristike modela za dva Mahova broja M∞ = 0,8 i 0,9 i otklon krmila δ=15°. Normalne sile koje se javljaju na modelu i krmilu merene su unutrašnjim aerovagama. Dobijeni rezultati merenja normalne sile na modelu i posebno na krmilu povezani su sa rezultatima vizualizacije strujanja metodom uljnih premaza. Priložene fotografije vizualizacije strujanja ilustruju promene strujanja oko modela i potvrđuju rezultate aerodinamičkih merenja. / Aerodynamic test results and boundary layer flow visualization on the wings and fins of laser guided bomb model are presented in this article. Test was performed in the T-38 wind tunnel. Influence of fin position relative to conic top of the model on aerodynamic characteristics was analyzed. Analysis was performed on aerodynamic characteristics of the model for two Mach numbers M∞ = 0,8 and 0,9 and fin deflection δ=15°. Normal forces present on the model and fin were measured by internal strain gauge balances. Obtained test results of normal force on the model and especially on the fin were associated with flow visualization results, which were performed by oil emulsion method. Presented images of flow visualization show changes of the flow around the model and confirm the results of the aerodynamic measurements.

  12. One-pot preparation of glutathione-silica hybrid monolith for mixed-mode capillary liquid chromatography based on "thiol-ene" click chemistry.

    Science.gov (United States)

    Lin, Zian; Tan, Xiaoqing; Yu, Ruifang; Lin, Jiashi; Yin, Xiaofei; Zhang, Lan; Yang, Huanghao

    2014-08-15

    A novel glutathione (GSH)-silica hybrid monolithic column synthesized via a combination of thiol-ene click reaction and one-pot process was described, where thiol-end GSH organic monomer and 2,2-azobisisobutyronitrile (AIBN) were mixed with hydrolyzed tetramethyloxysilane (TMOS) and γ-methacryloxypropyltrimethoxysilane (γ-MAPS) and then introduced into a fused-silica capillary for simultaneous polycondensation and "thiol-ene" click reaction to form the GSH-silica hybrid monolith. The effects of the molar ratio of TMOS/γ-MAPS, the amount of GSH, and the volume of porogen on the morphology, permeability and pore properties of the prepared GSH-silica hybrid monoliths were studied in detail. A uniform monolithic network with high porosity was obtained. A series of test compounds including alkylbenzenes, amides, and anilines were used to evaluate the retention behaviors of the GSH-silica hybrid monolithic column. The results demonstrated that the prepared GSH-silica hybrid monolith exhibited multiple interactions including hydrophobicity, hydrophilicity, as well as cation exchange interaction. The run-to-run, column-to-column and batch-to-batch reproducibilities of the GSH-silica hybrid monolith for phenols' retention were satisfactory with the relative standard deviations (RSDs) less than 1.3% (n=5), 2.6% (n=3) and 3.2% (n=3), respectively, indicating the effectiveness and practicability of the proposed method. In addition, the GSH-silica hybrid monolith was applied to the separation of nucleotides, peptides and protein tryptic digests, respectively. The successful applications suggested the potential of the GSH-silica hybrid monolith in complex sample analysis. Copyright © 2014 Elsevier B.V. All rights reserved.

  13. The effect of 3β, 6β, 16β-trihydroxylup-20(29)-ene lupane compound isolated from Combretum leprosum Mart. on peripheral blood mononuclear cells.

    Science.gov (United States)

    Lacouth-Silva, Fabianne; Xavier, Caroline V; da S Setúbal, Sulamita; Pontes, Adriana S; Nery, Neriane M; de Castro, Onassis Boeri; Fernandes, Carla F C; Honda, Eduardo R; Zanchi, Fernando B; Calderon, Leonardo A; Stábeli, Rodrigo G; Soares, Andreimar M; Silva-Jardim, Izaltina; Facundo, Valdir A; Zuliani, Juliana P

    2015-11-25

    The Combretum leprosum Mart. plant, popularly known as mofumbo, is used in folk medicine for inflammation, pain and treatment of wounds. From this species, it is possible to isolate three triterpenes: (3β, 6β, 16β-trihydroxylup-20(29)-ene) called lupane, arjunolic acid and molic acid. In this study, through preclinical tests, the effect of lupane was evaluated on the cytotoxicity and on the ability to activate cellular function by the production of TNF-α, an inflammatory cytokine, and IL-10, an immuno regulatory cytokine was assessed. The effect of lupane on the enzymes topoisomerase I and II was also evaluated. For this reason, peripheral blood mononuclear cells (PBMCs) were obtained and cytotoxicity was assessed by the MTT method at three different times (1, 15 and 24 h), and different concentrations of lupane (0.3, 0.7, 1.5, 6, 3 and 12 μg/mL). The cell function was assessed by the production of TNF-α and IL-10 by PBMCs quantified by specific enzyme immunoassay (ELISA). The activity of topoisomerases was assayed by in vitro biological assays and in silico molecular docking. The results obtained showed that lupane at concentrations below 1.5 μg/mL was not toxic to the cells. Moreover, lupane was not able to activate cellular functions and did not alter the production of IL-10 and TNF-α. Furthermore, the data showed that lupane has neither interfered in the action of topoisomerase I nor in the action of topoisomerase II. Based on preclinical results obtained in this study, we highlight that the compound studied (lupane) has moderate cytotoxicity, does not induce the production of TNF-α and IL-10, and does not act on human topoisomerases. Based on the results of this study and taking into consideration the reports about the anti-inflammatory and leishmanicidal activity of 3β, 6β, 16β-trihydroxylup-20(29)-ene, we suggest that this compound may serve as a biotechnological tool for the treatment of leishmaniasis in the future.

  14. Origin and palaeoenvironmental significance of C25 and C27n-alk-1-enes in a 25,000-year lake-sedimentary record from equatorial East Africa

    Science.gov (United States)

    van Bree, L. G. J.; Rijpstra, W. I. C.; Cocquyt, C.; Al-Dhabi, N. A.; Verschuren, D.; Sinninghe Damsté, J. S.; de Leeuw, J. W.

    2014-11-01

    We studied the distribution of long-chain alkenes (n-C23 to n-C31) in well-dated sediments from Lake Challa, a deep crater lake near Mt. Kilimanjaro in equatorial East Africa, to reveal signatures of palaeo-environmental and palaeo-climatic changes affecting the production of these compounds during the last 25 kyr. The apolar fractions of organic sediment extracts dated to the last 16 kyr showed an unusual dominance of δ13C-depleted n-C25:1 and n-C27:1 alk-1-enes. These alkenes were not detected in soil and litter from near the shoreline and from the inner rim of the crater, pointing to an autochthonous, aquatic source. Analysis of suspended particulate matter indicated that the n-alk-1-enes are produced in the well-oxygenated upper 30 m of the water column, indicating a phytoplanktonic origin. Sedimenting particles collected monthly from December 2006 to November 2007 showed increased fluxes of n-alk-1-enes following the locally prominent short rain season in November-December. Green algae and/or cyanobacteria were identified as candidate sources of these alkenes. Production of the n-C25:1 and n-C27:1 alkenes in Lake Challa was much reduced during the Last Glacial Maximum and early late-glacial period, suggesting a temperature or CO2 effect on habitat suitability. We explored the potential of n-alk-1-ene accumulation rates, and of a derived Alkene Index [n-C27:1]/([n-C25:1] + [n-C27:1]), to record longer-term climatic changes. The Alkene Index record of Lake Challa over the past 25 kyr shows clear periodicity with a dominant frequency of ∼2.3 kyr, potentially indicative of monsoon variability directly or indirectly forced by variation in solar radiation.

  15. Effects of Bisphenol A Metabolite 4-Methyl-2,4-bis(4-hydroxyphenyl)pent-1-ene on Lung Function and Type 2 Pulmonary Alveolar Epithelial Cell Growth.

    Science.gov (United States)

    Liu, Shing-Hwa; Su, Chin-Chuan; Lee, Kuan-I; Chen, Ya-Wen

    2016-12-16

    Bisphenol A (BPA) is recognized as a major pollutant worldwide. 4-Methyl-2,4-bis(4-hydroxyphenyl)pent-1-ene (MBP) is a major active metabolite of BPA. The epidemiological and animal studies have reported that BPA is harmful to lung function. The role of MBP in lung dysfunction after BPA exposure still remains unclear. This study investigated whether MBP would induce lung alveolar cell damage and evaluated the role of MBP in the BPA exposure-induced lung dysfunction. An in vitro type 2 alveolar epithelial cell (L2) model and an ex vivo isolated reperfused rat lung model were used to determine the effects of BPA or MBP on cell growth and lung function. MBP, but not BPA, dose-dependently increased the mean artery pressure (Pa), pulmonary capillary pressure (Pc), pulmonary capillary filtration coefficient (Kfc), and wet/dry weight ratio in isolated reperfused rat lungs. MBP significantly reduced cell viability and induced caspases-3/7 cleavage and apoptosis and increased AMP-activated protein kinas (AMPK) phosphorylation and endoplasmic reticulum (ER) stress-related molecules expression in L2 cells, which could be reversed by AMPK-siRNA transfection. These findings demonstrated for the first time that MBP exposure induced type 2 alveolar cell apoptosis and lung dysfunction through an AMPK-regulated ER stress signaling pathway.

  16. Fast preparation of a highly efficient organic monolith via photo-initiated thiol-ene click polymerization for capillary liquid chromatography.

    Science.gov (United States)

    Chen, Lianfang; Ou, Junjie; Liu, Zhongshan; Lin, Hui; Wang, Hongwei; Dong, Jing; Zou, Hanfa

    2015-05-15

    A novel organic monolith was firstly prepared in a UV-transparent fused-silica capillary by a single-step approach via photo-initiated thiol-ene click polymerization reaction of 1,2,4-trivinylcyclohexane (TVCH) and pentaerythriol tetra(3-mercaptopropionate) (4SH) within 10min. The effects of both composition of prepolymerization solution and polymerization time on the morphology and permeability of monolithic column were investigated in detail. Then, the optimal condition was acquired to fabricate a homogeneous and permeable organic monolith. The chemical groups of the monolithic column were confirmed by Fourier transform infrared spectroscopy (FT-IR). The SEM graphs showed the organic monolith possessed a uniform porous structure, which promotes the highest column efficiency of ∼133,000 plates per meter for alkylbenzenes at the linear velocity of 0.65mm/s in reversed-phase liquid chromatography. Finally, the organic monolithic column was further applied for separation of basic compounds, pesticides and EPA610, indicating satisfactory separation ability. Copyright © 2015 Elsevier B.V. All rights reserved.

  17. The synthesis of weak acidic type hybrid monolith via thiol-ene click chemistry and its application in hydrophilic interaction chromatography.

    Science.gov (United States)

    Zeng, Jiao; Liu, Shengquan; Wang, Menglin; Yao, Shouzhuo; Chen, Yingzhuang

    2017-05-01

    In this work, a porous structure and good permeability monolithic column was polymerized in UV transparent fused-silica capillaries via photo-initiated thiol-ene click polymerization of 2,4,6,8-tetravinyl-2,4,6,8-tetramethylcyclotetrasiloxane (TMTVS), pentaerythritol tetra(3-mercaptopropionate)(PETMP), itaconic acid, respectively, in the presence of porogenic solvents (tetrahydrofuranand methanol) and an initiator (2,2-dimethoxy-2-phenylacetophenone) (DMPA) within 30 min. The physical properties of this monolith were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy and nitrogen adsorption/desorption measurements. For an overall evaluation of the monolith in chromatographic application, separations of polycyclic aromatic hydrocarbons (PAHs), phenols, amides and bases were carried out. The column efficiency of this monolith could be as high as 112 560 N/m. It also possesses a potential application in fabrication of monoliths with high efficiency for c-LC. In addition, the resulting monolithic column demonstrated the potential use in analysis of environment waters. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  18. Occurrence of (Z)-3,4-Dideoxyglucoson-3-ene in Different Types of Beer and Malt Beer as a Result of 3-Deoxyhexosone Interconversion.

    Science.gov (United States)

    Hellwig, Michael; Nobis, Arndt; Witte, Sophia; Henle, Thomas

    2016-04-06

    In beer, 3-deoxyglucosone (3-DG) and 3-deoxygalactosone (3-DGal) are important sugar degradation products, but little is known about the relevance of the interconversion reaction between these compounds in different types of beer. In the present study, 3-DG was quantitated at concentrations of 12.9-52.7 mg/L and 3-DGal at concentrations of 6.0-26.4 mg/L in different types of beer (pilsner, wheat, bock, dark, and alcohol-free beers). The concentrations in malt beer tended to be higher. Largely overlapping concentration ranges precluded a classification of beers by their 3-deoxyglycosone contents. 3,4-Dideoxyglucoson-3-ene (3,4-DGE) was identified as an important intermediate and quantitated in beer and malt beer for the first time. The E and Z isomers of the corresponding quinoxaline were synthesized by a new synthetic approach and isolated by semipreparative HPLC. An assay was developed for quantitation of (E)- and (Z)-3,4-DGE by HPLC-MS/MS, and the Z isomer was determined at concentrations of 0.3-1.7 mg/L in beer and 0.5-4.8 mg/L in malt beer samples. The E isomer was shown to be of little importance. Concentrations of 5-hydroxymethylfurfural (HMF) were twice as high as those of (Z)-3,4-DGE in beer samples (0.4-3.7 mg/L) but much higher in malt beer samples (1.6-336 mg/L).

  19. "One-step" preparation of thiol-ene clickable PEG-based thermoresponsive hyperbranched copolymer for in situ crosslinking hybrid hydrogel.

    Science.gov (United States)

    Dong, Yixiao; Saeed, Aram Omer; Hassan, Waqar; Keigher, Catherine; Zheng, Yu; Tai, Hongyun; Pandit, Abhay; Wang, Wenxin

    2012-01-01

    A well-defined poly(ethylene glycol) based hyperbranched thermoresponsive copolymer with high content of acrylate vinyl groups was synthesized via a "one-pot and one-step" deactivation enhanced atom transfer radical polymerization approach, which provided an injectable and in situ crosslinkable system via Michael-type thiol-ene reaction with a thiol-modified hyaluronan biopolymer. The hyperbranched structure, molecular weight, and percentage of vinyl content of the copolymer were characterized by gel permeation chromatography and (1)H NMR. The lower critical solution temperature of this copolymer is close to body temperature, which can result in a rapid thermal gelation at 37 °C. The scanning electron microscopy analysis of crosslinked hydrogel showed the network formation with porous structure, and 3D cell culture study demonstrated the good cell viability after the cells were embedded inside the hydrogel. This injectable and in situ crosslinking hybrid hydrogel system offers great promise as a new class of hybrid biomaterials for tissue engineering. Copyright © 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim.

  20. Critical Parameters and Critical-Region (p,ρ ,T) Data of trans-1,1,1,3-Tetrafluorobut-2-ene [HFO-1354mzy(E)

    Science.gov (United States)

    Kimura, Takeru; Kayukawa, Yohei; Miyamoto, Hiroyuki; Saito, Kiyoshi

    2017-08-01

    This study presents the experimental measurement of the pρ T properties and critical parameters of a low GWP type refrigerant, trans-1,1,1,3-Tetrafluorobut-2-ene (HFO-1354mzy(E)). The sample purity of the substance was 99 area %. p ρ T property measurements and visual observations of the meniscus of HFO-1354mzy(E) were carried out using a metal-bellows volumometer with an optical cell. The critical temperature was determined by observation of the critical opalescence. The critical pressure and critical density were determined as the inflection point of the isothermal p ρ T property data at the critical temperature. For more precise clarification of the thermodynamic surface in the vicinity of the critical point, additional p ρ T property measurements were carried out on three isotherms in the supercritical region. The expanded uncertainties (k = 2) in the temperature, pressure, and density measurements were estimated to be less than 3 mK, 1.2 kPa, and 0.32 \\hbox {kg} \\cdot \\hbox {m}^{-3}, respectively. The expanded uncertainties of the critical parameters were estimated to be less than 13 mK, 1.4 kPa, and 2.3 \\hbox {kg} \\cdot \\hbox {m}^{-3}, respectively. These values are the first reported for HFO-1354mzy(E) and are necessary for the development of its equation of state in the near future.

  1. Thiol-ene immobilisation of carbohydrates onto glass slides as a simple alternative to gold-thiol monolayers, amines or lipid binding.

    Science.gov (United States)

    Biggs, Caroline I; Edmondson, Steve; Gibson, Matthew I

    2015-01-01

    Carbohydrate arrays are a vital tool in studying infection, probing the mechanisms of bacterial, viral and toxin adhesion and the development of new treatments, by mimicking the structure of the glycocalyx. Current methods rely on the formation of monolayers of carbohydrates that have been chemically modified with a linker to enable interaction with a functionalised surface. This includes amines, biotin, lipids or thiols. Thiol-addition to gold to form self-assembled monolayers is perhaps the simplest method for immobilisation as thiolated glycans are readily accessible from reducing carbohydrates in a single step, but are limited to gold surfaces. Here we have developed a quick and versatile methodology which enables the use of thiolated carbohydrates to be immobilised as monolayers directly onto acrylate-functional glass slides via a 'thiol-ene'/Michael-type reaction. By combining the ease of thiol chemistry with glass slides, which are compatible with microarray scanners this offers a cost effective, but also useful method to assemble arrays.

  2. IN0523 (Urs-12-ene-3α,24β-diol) a plant based derivative of boswellic acid protect Cisplatin induced urogenital toxicity.

    Science.gov (United States)

    Singh, Amarinder; Arvinda, S; Singh, Surjeet; Suri, Jyotsna; Koul, Surinder; Mondhe, Dilip M; Singh, Gurdarshan; Vishwakarma, Ram

    2017-03-01

    The limiting factor for the use of Cisplatin in the treatment of different type of cancers is its toxicity and more specifically urogenital toxicity. Oxidative stress is a well-known phenomenon associated with Cisplatin toxicity. However, in Cisplatin treated group, abnormal animal behavior, decreased body weight, cellular and sub-cellular changes in the kidney and sperm abnormality were observed. Our investigation revealed that Cisplatin when administered in combination with a natural product derivative (Urs-12-ene-3α,24β-diol, labeled as IN0523) resulted in significant restoration of body weight and protection against the pathological alteration caused by Cisplatin to kidney and testis. Sperm count and motility were significantly restored near to normal. Cisplatin caused depletion of defense system i.e. glutathione peroxidase, catalase and superoxide dismutase, which were restored close to normal by treatment of IN0523. Reduction in excessive lipid peroxidation induced by Cisplatin was also found by treatment with IN0523. The result suggests that IN0523 is a potential candidate for ameliorating Cisplatin induced toxicity in the kidney and testes at a dose of 100mg/kg p.o. via inhibiting the oxidative stress/redox status imbalance and may be improving the efflux mechanism. Copyright © 2017 Elsevier Inc. All rights reserved.

  3. Preparation and characterization of hydrophilic hybrid monoliths via thiol-ene click polymerization and their applications in chromatographic analysis and glycopeptides enrichment.

    Science.gov (United States)

    Bai, Jingyao; Liu, Zhongshan; Wang, Hongwei; You, Xin; Ou, Junjie; Shen, Yehua; Ye, Mingliang

    2017-05-19

    A macroporous hydrophilic organic-silica hybrid monolithic column was synthesized via photoinitiated thiol-ene click polymerization reaction of 1-thioglycerol-modified polyhedral oligomeric vinylsilsesquioxane (vinylPOSS) and dithiothreitol (DTT) in a binary porogenic system consisting of tetrahydrofuran (THF) and dodecanol. 1-Thioglycerol was used to modify vinylPOSS in order to form a precursor with good solubility in the binary porogenic system. The influences of both the ratio of 1-thioglycerol/vinylPOSS and the porogenic solvents on the morphology and permeability of hybrid monoliths were studied in detail. The physical properties of hybrid monolith were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy and nitrogen adsorption/desorption measurement. The chromatographic performance was evaluated by separation of neutral polar compounds in capillary liquid chromatography (cLC). The resulting column possessed homogeneous macroporous structure and showed hydrophilic interaction liquid chromatography (HILIC) separation mechanism with high efficiency of 65,000Nm -1 for formamide. Ultimately, the hybrid matrix was grafted with hydrazine groups and then exhibited the ability of glycopeptides enrichment. Copyright © 2016 Elsevier B.V. All rights reserved.

  4. One-Pot Multicomponent Synthesis of Glycopolymers through a Combination of Host-Guest Interaction, Thiol-ene, and Copper-Catalyzed Click Reaction in Water.

    Science.gov (United States)

    Hu, Xiang; Gao, Jinbo; Luo, Yan; Wei, Ting; Dong, Yishi; Chen, Gaojian; Chen, Hong

    2017-10-01

    There is a common phenomenon that the heterogeneity of natural oligosaccharides contains various sugar units, which can be used to enhance affinity and selectivity toward a specific receptor, so the synthesis of heterogeneous glycopolymers is always an important issue in the glycopolymer field. Herein, this study conducts a one-pot method to prepare polyrotaxane-based heteroglycopolymers anchored with different sugar units and fluorescent moieties via the combination of host-guest interaction, thiol-ene, and copper-catalyzed click chemistry in water. Fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, gel permeation chromatography, X-ray diffraction, and Ellman's assay test are used in the paper to characterize the compounds. Quartz crystal microbalance-dissipation (QCD-D) experiments and bacterial adhesion assay are utilized to study the interactions of polyrotaxane-based heteroglycopolymers with Con A and Escherichia coli. The results reveal that polyrotaxanes (PRs) with mannose and glucose present better specificity toward Con A and E. coli than PRs with glucose due to synergistic effects. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  5. One-pot preparation of mercaptotetrazole-silica hybrid monoliths by the thiol-ene click reaction for mixed-mode capillary liquid chromatography.

    Science.gov (United States)

    Wang, Haojiang; Hu, Wenli; Zheng, Qiong; Bian, Wei; Lin, Zian

    2017-06-01

    A novel mercaptotetrazole-silica hybrid monolithic column was prepared for capillary liquid chromatography, in which the thiol-end mercaptotetrazole was mixed with hydrolyzed γ-methacryloxypropyltrimethoxysilane and tetramethyloxysilane for the co-polycondensation and thiol-ene click reaction in a one-pot process. The effects of the molar ratio of silanes, the amount of mercaptotetrazole, and the volume of porogen on the morphology, permeability and pore properties of the as-prepared mercaptotetrazole-silica hybrid monoliths were investigated in detail. A series of test compounds including alkylbenzenes, amides and anilines were employed for evaluating the retention behaviors of the mercaptotetrazole-silica hybrid monolithic columns. The results demonstrated that the mercaptotetrazole-silica hybrid monoliths exhibited hydrophobic, hydrophilic as well as ion-exchange interaction. The run-to-run, column-to-column and batch-to-batch reproducibilities of the mercaptotetrazole-silica hybrid monoliths were satisfactory with the relative standard deviations less than 1.4 (n = 5), 3.9 (n = 3) and 4.0% (n = 5), respectively. In addition, the mercaptotetrazole-silica hybrid monolith was further applied to the separation of sulfonamides, nucleobases and protein tryptic digests. These successful applications confirmed the promising potential of the mercaptotetrazole-silica hybrid monolith in the separation of complex samples. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  6. Facile "one-pot" synthesis of poly(methacrylic acid)-based hybrid monolith via thiol-ene click reaction for hydrophilic interaction chromatography.

    Science.gov (United States)

    Lv, Xumei; Tan, Wangming; Chen, Ye; Chen, Yingzhuang; Ma, Ming; Chen, Bo; Yao, Shouzhuo

    2016-07-08

    A novel sol-gel "one-pot" approach in tandem with a radical-mediated thiol-ene reaction for the synthesis of a methacrylic acid-based hybrid monolith was developed. The polymerization monomers, tetramethoxysilane (TMOS) and 3-mercaptopropyl trimethoxysilane (MPTS), were hydrolyzed in high-concentration methacrylic acid solution that also served as a hydrophilic functional monomer. The resulting solution was then mixed with initiator (2, 2'-azobis (2-methylpropionamide) dihydrochloride) and porogen (urea, polyethylene glycol 20,000) in a capillary column and polymerized in water bath. The column had a uniform porous structure and a good permeability. The evaluation of the monolith was performed by separation of small molecules including nucleosides, phenols, amides, bases and Triton X-100. The calibration curves for uridine, inosine, adenosine and cytidine were determined. All the calibration curves exhibited good linear regressions (R(2)≥0.995) within the test ranges of 0.5-40μg/mL for four nucleosides. Additionaliy, atypical hydrophilic mechanism was proved by elution order from low to high according to polarity retention time increased with increases in the content of the organic solvent in the mobile phase. Further studies indicated that hydrogen bond and electrostatic interactions existed between the polar analytes and the stationary phase. This was the mechanism of retention. The excellent separation of the BSA digest showed good hydrophility of the column and indicated the potential in separation of complex biological samples. Copyright © 2016 Elsevier B.V. All rights reserved.

  7. Crystal and Molecular Structure Studies of Ethyl 4-(4-Hydroxyphenyl-6-(6-methoxy-2-naphthyl-2-oxocyclohex-3-ene-1-carboxylate and Ethyl 4-(3-Bromophenyl-6-(6-methoxy-2-naphthyl-2-oxocyclohex-3-ene-1-carboxylate

    Directory of Open Access Journals (Sweden)

    Badiadka Narayana

    2012-08-01

    Full Text Available The crystal and molecular structures of the title compounds, ethyl 4-(4-hydroxyphenyl-6-(6-methoxy-2-naphthyl-2-oxocyclohex-3-ene-1-carboxylate (I and ethyl 4-(3-bromophenyl-6-(6-methoxy-2-naphthyl-2-oxocyclohex-3-ene-1-carboxylate (II, are reported and confirmed by single crystal X-ray diffraction data. Compound (I, C26H24O5, crystallizes from a methanol solution in the monoclinic C2/c space group with eight molecules in the unit cell. The unit cell parameters are: a = 25.4114(5 Å, b = 8.47440(10 Å, c = 20.6921(4 Å, β = 108.328(2° and V = 4229.92(13 Å3. Disorder is observed throughout the entire molecule with an occupancy ratio 0.690(2:0.310(2. Compound (II, C26H23O4Br, crystallizes from an ethyl acetate solution in the monoclinic P21/c spacegroup with four molecules in the unit cell. The unit cell parameters are a = 17.8991(9 Å, b = 11.4369(6 Å, c = 10.8507(5 Å, β = 92.428(4° and V = 2219.25(19 Å3. Disorder is observed in the cyclohexenone ring and the carboxylate group with an occupancy ratio 0.830(6:0.170(6. Weak O–H...O (I or C–H...O (II intermolecular interactions are observed which influence crystal packing stability. These chalcone derivative types of molecules are important in their ability to act as activated unsaturated systems in conjugated addition reactions of carbanions in the presence of basic catalysts which exhibit a multitude of biological activities.

  8. Interactions of butane, but-2-ene or xylene-like linked bispyridinium para-aldoximes with native and tabun-inhibited human cholinesterases.

    Science.gov (United States)

    Calić, Maja; Bosak, Anita; Kuca, Kamil; Kovarik, Zrinka

    2008-09-25

    Kinetic parameters were evaluated for inhibition of native and reactivation of tabun-inhibited human erythrocyte acetylcholinesterase (AChE, EC 3.1.1.7) and human plasma butyrylcholinesterase (BChE, EC 3.1.1.8) by three bispyridinium para-aldoximes with butane (K074), but-2-ene (K075) or xylene-like linker (K114). Tested aldoximes reversibly inhibited both cholinesterases with the preference for binding to the native AChE. Both cholinesterases showed the highest affinity for K114 (K(i) was 0.01 mM for AChE and 0.06 mM for BChE). The reactivation of tabun-inhibited AChE was efficient by K074 and K075. Their overall reactivation rate constants were around 2000 min(-1)M(-1), which is seven times higher than for the classical bispyridinium para-aldoxime TMB-4. The reactivation of tabun-inhibited AChE assisted by K114 was slow and reached 90% after 20 h. Since the aldoxime binding affinity of tabun-inhibited AChE was similar for all tested aldoximes (and corresponded to their K(i)), the rate of the nucleophilic displacement of the phosphoryl-moiety from the active site serine was the limiting factor for AChE reactivation. On the other hand, none of the aldoximes displayed a significant reactivation of tabun-inhibited BChE. Even after 20 h, the reactivation maximum was 60% for 1 mM K074 and K075, and only 20% for 1 mM K114. However, lower BChE affinities for K074 and K075 compared to AChE suggest that the fast tabun-inhibited AChE reactivation by these compounds would not be obstructed by their interactions with BChE in vivo.

  9. C60 and Sc3N@C80(TMB-PPO derivatives as constituents of singlet oxygen generating, thiol-ene polymer nanocomposites

    Directory of Open Access Journals (Sweden)

    Ashli R. Toles

    2016-07-01

    Full Text Available Numerous functionalization methods have been employed to increase the solubility, and therefore, the processability of fullerenes in composite structures, and of these radical addition reactions continue to be an important methodology. C60 and Sc3N@C80 derivatives were prepared via radical addition of the photodecomposition products from the commercial photoinitiator TMB-PPO, yielding C60(TMB-PPO5 and Sc3N@C80(TMB-PPO3 as preferred soluble derivatives obtained in high yields. Characterization of the mixture of isomers using standard techniques suggests an overall 1PPO:6TMB ratio of addends, reflecting the increased reactivity of the carbon radical. Although, a higher percentage of PPO is observed in the Sc3N@C80(TMB-PPO3 population, perhaps due to reverse electronic requirements of the substrate. Visually dispersed thiol-ene nanocomposites with low extractables were prepared using two monomer compositions (PETMP:TTT and TMPMP:TMPDE with increasing fullerene derivative loading to probe network structure-property relationships. Thermal stability of the derivatives and the resulting networks decreased with increased functionality and at high fullerene loadings, respectively. TMPMP:TMPDE composite networks show well-dispersed derivatives via TEM imaging, and increasing Tg’s with fullerene loading, as expected for the incorporation of a more rigid network component. PETMP:TTT composites show phase separation in TEM, which is supported by the observed Tg’s. Singlet oxygen generation of the derivatives decreases with increased functionality; however, this is compensated for by the tremendous increase in solubility in organic solvents and miscibility with monomers. Most importantly, singlet oxygen generation from the composites increased with fullerene derivative loading, with good photostability of the networks.

  10. Studies directed towards a mechanistic evaluation of inactivation of aromatase by the suicide substrates androsta-1,4-diene-3,17-diones and its 6-ene derivatives aromatase inactivation by the 19-substituted derivatives and their enzymic aromatization.

    Science.gov (United States)

    Numazawa, Mitsuteru; Nagaoka, Masao; Handa, Wakako; Ogawa, Yoko; Matsuoka, Satomi

    2007-01-01

    To gain insight into the mechanistic features for aromatase inactivation by the typical suicide substrates, androsta-1,4-diene-3,17-dione (ADD, 1) and its 6-ene derivative 2, we synthesized 19-substituted (methyl and halogeno) ADD and 1,4,6-triene derivatives 8 and 10 along with 4,6-diene derivatives 9 and tested for their ability to inhibit aromatase in human placental microsomes as well as their ability to serve as a substrate for the enzyme. 19-Methyl-substituted steroids were the most powerful competitive inhibitors of aromatase (K(i): 8.2-40 nM) in each series. Among the 19-substituted inhibitors examined, 19-chloro-ADD and its 6-ene derivatives (7b and 9b) inactivated aromatase in a time-dependent manner in the presence of NADPH in air while the other ones did not. The time-dependent inactivation was blocked by the substrate AD and required NADPH. Only the time-dependent inactivators 7b and 9b in series of 1,4-diene and 1,4,6-triene steroids as well as all of 4,6-diene steroids 9, except for the methyl compound 9a, served as a substrate for aromatase to yield estradiol and/or its 6-ene estradiol with lower conversion rates compared to the corresponding parent steroids 1,4-diene, 1,4,6-triene and 4,6-diene derivatives. The present findings strongly suggest that the aromatase reaction, 19-oxygenation, at least in part, would be involved in the time-dependent inactivation of aromatase by the suicide substrates 1 and 2, where the 19-substitutent would play a critical role in the aromatase reaction probably though steric and electronic reasons.

  11. Aproximación a la situación de salud en tres comunidades Asháninkas ubicadas en las cuencas del río Tambo y Ene, 2006

    OpenAIRE

    Carolina Tarqui-Mamani; Javier Vargas-Herrera; Elisa Terreros-Martínez; Walter Torres

    2009-01-01

    En mayo de 2006 se realizó un estudio transversal en tres comunidades Asháninkas ubicadas en las cuencas de los ríos Tambo y Ene, para describir su situación de salud. Se definió la situación salud sobre la base de cinco indicadores: prevalencia de la desnutrición infantil, enfermedades diarreicas agudas (EDA), infecciones respiratorias agudas (IRA), mortalidad materna y mortalidad infantil. Se interrogó a los padres o madres de familia acerca de defunciones en menores de 1 año, defunciones m...

  12. Kas oleksite nõus koolidirektorina võtma endale karistusõigust, seega õigust õpilasi trahvida? / Ojaveer, Ott; Tannberg, Ene; Kikas, Peeter; Braziulane, Alina; Samm, Toomas; Reinson, Jaan; Asser, Hiie; Kokk, Undel; Serk, Ants; Ahven, Ruth

    Index Scriptorium Estoniae

    2008-01-01

    Küsimusele vastavad Hugo Treffneri gümnaasiumi direktor Ott Ojaveer, Miina Härma gümnaasiumi direktor Ene Tannberg, Tartu kunstigümnaasiumi direktor Peeter Kikas, Vene lütseumi direktor Alina Braziulane, Mart Reiniku gümnaasiumi direktor Toomas Samm, Descantes'i lütseumi direktor Jaan Reinson, Annelinna gümnaasiumi direktor Hiie Asser, Karlova gümnaasiumi direktor Undel Kokk, Kivilinna gümnaasiumi direktor Ants Serk, Veeriku kooli direktor Ruth Ahven

  13. Synphos modified Pt nanoclusters, their heterogenization by silica sol-gel entrapment, and catalytic activity in hydrogenolysis of bicyclo[2.2.2]oct-7-enes and hydrogenation of ethyl pyruvate

    Energy Technology Data Exchange (ETDEWEB)

    Neatu, F; Parvulescu, V I [Faculty of Chemistry, Department of Chemical Technology and Catalysis, University of Bucharest, B-dul Regina Elisabeta 4-12, Bucharest 030018 (Romania); Kraynov, A [Jacobs University Bremen, Campus Ring 8, D-28759 Bremen (Germany); Kranjc, K; Kocevar, M [Faculty of Chemistry and Chemical Technology, University of Ljubljana, Askerceva 5, SI-1000 Ljubljana (Slovenia); Ratovelomanana-Vidal, V [Laboratoire de Synthese Selective Organique et Produits Naturels, Ecole Nationale Superieure de Chimie de Paris, UMR 7573 CNRS, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05 (France); Richards, R [Department of Chemistry and Geochemistry, Colorado School of Mines, 1500 Illiniois, Golden, CO 80401 (United States)], E-mail: v_parvulescu@chem.unibuc.ro, E-mail: virginie-vidal@enscp.fr, E-mail: rrichard@mines.edu

    2008-06-04

    Platinum (Pt) colloids modified by the chiral ligand synphos were prepared with the goal of obtaining a catalytic nanomaterial and were subsequently embedded in silica to form a heterogeneous catalyst. The systems were characterized by {sup 31}P-NMR, x-ray diffraction, molecular modeling and diffuse reflectance infrared Fourier transform spectroscopy (DRIFTs) measurements. These colloids, both as 'quasi-homogeneous catalysts' (or soluble heterogeneous catalysts) and embedded in silica (heterogeneous catalysts) were employed in the selective hydrogenolysis of highly sterically constrained bicyclo[2.2.2]oct-7-enes and hydrogenation of ethyl pyruvate.

  14. Heat of Mixing and Solution of Hex-1-ene C6H12 + C6H18N3OP Phosphoric tris(dimethylamide) (HMSD1111, LB3772_H)

    Science.gov (United States)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume B 'Binary Liquid Systems of Nonelectrolytes II' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Heat of Mixing and Solution of Hex-1-ene C6H12 + C6H18N3OP Phosphoric tris(dimethylamide) (HMSD1111, LB3772_H)' providing data from direct low-pressure calorimetric measurement of molar excess enthalpy at variable mole fraction and constant temperature.

  15. Preparation of a novel weak cation exchange/hydrophobic interaction chromatography dual-function polymer-based stationary phase for protein separation using "thiol-ene click chemistry".

    Science.gov (United States)

    Yang, Fan; Bai, Quan; Zhao, Kailou; Gao, Dong; Tian, Lei

    2015-02-01

    A novel dual-function mixed-mode stationary phase based on poly(glycidyl methacrylate-co-ethylene dimethacrylate) microspheres was synthesized by thiol-ene click chemistry and characterized by infrared spectroscopy and elemental analysis. The new system displays both hydrophobic interaction chromatography (HIC) character in a high salt concentration mobile phase, and weak cation exchange (WCX) chromatography character in a low salt concentration mobile phase. It can be used to separate proteins in both ion-exchange chromatography (IEC) mode and HIC mode. The resolution and selectivity of the stationary phase were evaluated in both HIC mode and IEC mode using protein standards. In comparison with the conventional WCX and HIC columns, the results were satisfactory and acceptable. Protein mass and bioactivity recoveries of more than 96% can be achieved in both HIC mode and IEC mode using this column. The results indicate that the novel dual-function mixed-mode column in many cases can replace the use of two individual WCX and HIC columns. In addition, the effects on protein separation of different ligand structures in the dual-function stationary phase and the pH of the mobile phase used were also investigated in detail. The results show that electrostatic interaction of the ligand with proteins must match the hydrophobicity of the ligand, which is an important factor to prepare the dual-function stationary phase. On the basis of this dual-function mixed-mode chromatography column, a new two-dimensional liquid chromatography technology with a single column system was also developed in this study, and was used to renature and purify recombinant human interferon-γ from inclusion bodies. The mass recovery, purity, and specific bioactivity obtained for the purified recombinant human interferon-γ were 87.2%, 92.4%, and 2.8 × 10(7) IU/mg, respectively, in IEC mode, and 83.4%, 95.2%, and 4.3 × 10(7) IU/mg, respectively, in HIC mode. The results indicate that the

  16. Zeytinli Ada İskelet Topluluğunun Diş ve Çene Patolojisi Açısından İncelenmesi

    Directory of Open Access Journals (Sweden)

    Saadet BIÇAK

    2015-03-01

    Full Text Available Özet. Zeytinli ada, Balıkesir ili Erdek ilçesi sahiline yaklaşık olarak 250 metre uzaklıkta yer alıp 7900 metrekarelik bir alanı kapsar. Zeytinli ada arkeolojik buluntuları açısından oldukça zengin bir tarihe ve aynı zamanda da Helen-Roma-Bizans ve Osmanlı dönemlerini içeren mozaik bir yapıya sahiptir. Çalışma materyalini, 2007-2011 yılları arasında gerçekleştirilen kazılar sonucunda ortaya çıkarılan 18 kadın, 37 erkek, 11 bebek ve çocuk, 60’ı cinsiyeti bilinmeyen olmak üzere toplam 126 bireye ait çene ve dişler oluşturmaktadır. Bireylere ait 467 diş üzerinde çürük, aşınma, apse, ölüm öncesi diş kaybı (antemortem, hypoplasia, alveol kaybı ve diş taşına bakılarak bunların bireylere ve cinsiyetlere göre dağılımları ortaya konulmaya çalışılmıştır. Abstract. Zeytinli ada, being far away almost 250 meters from the Coast of Erdekli in Balıkesir, takes in the area of 7900 sqm. Zeytinli Ada has a rich history in the view of archeological finds and also has a mosaic structure including the periods of Hellen-Rome- Byzantine and Ottoman. The study materials consist of jaws and teeth belonging to total 126 people ; 18 women, 38 men, 11 infants and  children, 60 unknown  sex  having been  come into existence in the result of excavations between 2007-2011 years.  It is studied to determine the distribution according to the gender and individuals by examining decay, abrasion, abscess, the tooth loss before death (antemortem, hypoplasia, alveolar loss, and dental stone on 467 teeth belonging to individuals.

  17. Kas Ene Ergmast saab president? / Ene Ergma ; interv. Peeter Ernits

    Index Scriptorium Estoniae

    Ergma, Ene, 1944-

    2006-01-01

    Riigikogu aseesimees räägib presidendivalimistel osalemisest, presidendile esitatavatest nõuetest, oma väidetavast kunagisest seotusest Nõukogude eriteenistusega, hariduse tähtsusest Eesti arengus ja Res Publica käekäigust

  18. 2-Morpholinoisoflav-3-enes as flexible intermediates in the synthesis of phenoxodiol, isophenoxodiol, equol and analogues: vasorelaxant properties, estrogen receptor binding and Rho/RhoA kinase pathway inhibition.

    Science.gov (United States)

    Tilley, Andrew J; Zanatta, Shannon D; Qin, Cheng Xue; Kim, In-Kyeom; Seok, Young-Mi; Stewart, Alastair; Woodman, Owen L; Williams, Spencer J

    2012-04-01

    Isoflavone consumption correlates with reduced rates of cardiovascular disease. Epidemiological studies and clinical data provide evidence that isoflavone metabolites, such as the isoflavan equol, contribute to these beneficial effects. In this study we developed a new route to isoflavans and isoflavenes via 2-morpholinoisoflavenes derived from a condensation reaction of phenylacetaldehydes, salicylaldehydes and morpholine. We report the synthesis of the isoflavans equol and deoxygenated analogues, and the isoflavenes 7,4'-dihydroxyisoflav-3-ene (phenoxodiol, haganin E) and 7,4'-dihydroxyisoflav-2-ene (isophenoxodiol). Vascular pharmacology studies reveal that all oxygenated isoflavans and isoflavenes can attenuate phenylephrine-induced vasoconstriction, which was unaffected by the estrogen receptor antagonist ICI 182,780. Furthermore, the compounds inhibited U46619 (a thromboxane A(2) analogue) induced vasoconstriction in endothelium-denuded rat aortae, and reduced the formation of GTP RhoA, with the effects being greatest for equol and phenoxodiol. Ligand displacement studies of rat uterine cytosol estrogen receptor revealed the compounds to be generally weak binders. These data are consistent with the vasorelaxation activity of equol and phenoxodiol deriving at least in part by inhibition of the RhoA/Rho-kinase pathway, and along with the limited estrogen receptor affinity supports a role for equol and phenoxodiol as useful agents for maintaining cardiovascular function with limited estrogenic effects. Copyright © 2012 Elsevier Ltd. All rights reserved.

  19. Estimación del impacto económico de los museos a través de las nuevas tecnologías de la información: el caso del museo Ene.Térmica

    Directory of Open Access Journals (Sweden)

    Vicente COLL-SERRANO

    Full Text Available Cada vez es más necesario poner de relieve el potencial de la cultura como motor del desarrollo económico y su capacidad para generar ingresos y crear empleo. Por este motivo, en este trabajo se evalúa el impacto económico del museo Ene.Térmica sobre el territorio en el que se ubica, Ponferrada (Comunidad de Castilla y León, España. Para lograr este objetivo, la estimación de los distintos tipos de efectos necesarios para determinar el impacto de las actividades económicas vinculas a la existencia de Ene.Térmica se realiza, en base a la metodología de las Tablas Input-Output, mediante la herramienta Impactrimonio, una aplicación implementada sobre una plataforma web. Los resultados obtenidos indican que entre las actividades del museo y los flujos de demanda desplegados por el efecto arrastre de los visitantes se generó, entre los años 2011 y 2012, un incremento de la producción de más de 15 millones de euros, un incremento de las rentas de la población de casi 10 millones de euros y un aumento de la ocupación de 327 empleos.

  20. In-situ functionalized monolithic polysiloxane-polymethacrylate composite materials from polythiol-ene double click reaction in capillary column format for enantioselective nano-high-performance liquid chromatography.

    Science.gov (United States)

    Wolter, Marc; Lämmerhofer, Michael

    2017-05-12

    This work reports on the proof-of-principle of preparation of novel one step in-situ functionalized monolithic polysiloxane-polymethacrylate composite materials in capillary columns for enantioselective nano-HPLC using a thiol-ene click reaction. Quinine carbamate as functional monomer and ethylene dimethacrylate as crosslinker were both used as ene components in a thermally initiated double click-type polymerization reaction with poly(3-mercaptopropyl)methylsiloxane as thiol component in presence of 1-propanol as porogenic solvent. Elemental analysis and on-capillary fluorescence measurement proved the successful incorporation of the functional chiral monomer into the polymer. Scanning electron microscopy images revealed a macroporous polymer morphology which is typical for a nucleation and growth mechanism of pore formation. The individual microglobules appear relatively spherical and smooth indicating a non-porous nature. Nano-HPLC experiments of the chiral monolithic capillary column provided successful enantiomer separation of N-3,5-dinitrobenzoylleucine as test compound in polar organic elution mode clearly documenting the successful implementation of the proposed concept towards new functionalized monolithic composite materials. Copyright © 2017 Elsevier B.V. All rights reserved.

  1. Crystal structure of a compact three-dimensional metal-organic framework based on Cs(+) and (4,5-di-cyano-1,2-phenyl-ene)bis-(phospho-nic acid).

    Science.gov (United States)

    Mendes, Ricardo F; Venkatramaiah, Nutalapati; Tomé, João P C; Almeida Paz, Filipe A

    2016-12-01

    A new metal-organic framework compound, poly[[μ7-dihydrogen (4,5-di-cyano-1,2-phenyl-ene)diphospho-nato]-(oxonium)caesium], [Cs(C8H4N2O6P2)(H3O)] n (I), based on Cs(+) and the organic linker 4,5-di-cyano-1,2-phenyl-ene)bis-(phospho-nic acid, (H4cpp), containing two distinct coordinating functional groups, has been prepared by a simple diffusion method and its crystal structure is reported. The coordination polymeric structure is based on a CsO8N2 complex unit comprising a monodentate hydro-nium cation, seven O-atom donors from two phospho-nium groups of the (H2cpp)(2-) ligand, and two N-atom donors from bridging cyano groups. The high level of connectivity from both the metal cation and the organic linker allow the formation of a compact and dense three-dimensional network without any crystallization solvent. Topologically (I) is a seven-connected uninodal network with an overall Schäfli symbol of {4(17).6(4)}. Metal cations form an undulating inorganic layer, which is linked by strong and highly directional O-H⋯O hydrogen-bonding inter-actions. These metallic layers are, in turn, connected by the organic ligands along the [010] direction to form the overall three-dimensional framework structure.

  2. LEADER-tegevusest / Ene Sarapuu

    Index Scriptorium Estoniae

    Sarapuu, Ene

    2009-01-01

    Läänemaa LEADER-tegevusgrupi eesmärk on maakonna kui terviku ühtne areng, et kogu Läänemaa oleks vajalike teenustega kaetud ning ettevõtjad julgeks ning suudaks uute teenuste ja toodetega turule tulla

  3. Parim personaliprojekt 2014 / Ene Olle

    Index Scriptorium Estoniae

    Olle, Ene

    2014-01-01

    Personalijuhtimise ühingu PARE konverentsil "Töötajat väärtustav organisatsioon" pälvis tiitli "Parim personaliprojekt 2013" ettevõtte ISS Eesti erivajadustega inimeste värbamise projekt. Teistest konkursil osalenud projektidest

  4. Messiuudised : Maison & Objet / Ene Jakobi

    Index Scriptorium Estoniae

    Jakobi, Ene

    2008-01-01

    Uusi trende kõige suuremalt disainimessilt Maison & Objet Prantsusmaal - Jean-Charles de Castelbajaci joonistatud toolid, Jean-Christophe Bernardi pleksiklaasist tool ja laud, Joseph Licciardi ja Paul Coheni-Philipp Veenendaali etanoolil põlevad kaminad, Philippe Lebru kellad, Vincenzo Antonuccio vaasid ja Anna Siedlecka ning Radek Achramoviczi täispuhutavad valgustid

  5. Inimlikkus ja erootika / Ene Lamp

    Index Scriptorium Estoniae

    Lamp, Ene

    1998-01-01

    Erootilise motiivi toimimisest eelmise ja praeguse sajandivahetuse kunstis Pärnu Uue Kunsti Muuseumi korraldatud näituste - Gustav Klimti ja Egon Schiele akvarellide ja joonistuste faksiimiletest, Jean Rustini maalidest - põhjal. Erotismi jõudmisest eesti kunsti (Triik, Vabbe, Wiiralt)

  6. Paleoenvironmental significance of aluminum phosphate-sulfate minerals in the upper Cretaceous ooidal ironstones, E-NE Aswan area, southern Egypt

    Science.gov (United States)

    Salama, Walid

    2014-09-01

    Aluminum phosphate-sulfate (APS) minerals are present as small, disseminated crystals in the upper Cretaceous shallow marine ooidal ironstones, E-NE Aswan area, southern Egypt. Their association with the ironstones is considered as a proxy of subaerial weathering and post-diagenetic meteoric water alteration. The mineralogical composition of the ooidal ironstones was investigated by optical and scanning electron microscopes, X-ray diffraction, Fourier transform infrared and Raman spectroscopy. The ooidal ironstones are composed mainly of ooids and groundmass, both of which consist of a mixture of detrital (quartz) and diagenetic (fluorapatite, chamosite and pyrite) mineral assemblages. These mineral assemblages are destabilized under acidic and oxidizing, continental conditions. These conditions resulted from the oxidation of pyrite and probably organic matter under warm and humid, tropical climate followed the Santonian Sea regression and subaerial exposure. These pedogenic conditions promoted corrosion of quartz, dissolution of chamosite and apatite and hydrolysis of feldspars of the nearby exposed granitoids. The released Si, Al and Sr from quartz, chamosite and feldspars; Fe and S from pyrite and P, Ca and light rare earth elements (LREE) from apatite are reprecipitated as hematite, kaolinite, apatite and APS minerals from the pore fluids or along fractures. The paragenetic sequence and textural relationships of this post-diagenetic mineral assemblage indicate that hematite was formed by replacement of chamosite followed by formation of a secondary generation of pore filling chlorapatite and APS minerals and finally the precipitation of kaolinite in the remaining pore spaces. The formation of APS minerals and chlorapatite is simultaneous, but APS minerals are stable at shallow depths under acidic to neutral pH conditions, whereas chlorapatite is stable under alkaline pH conditions. Alkaline conditions were maintained at greater depths when the infiltrated

  7. Anti-inflammatory effect of triterpene 3β, 6β, 16β-trihydroxylup-20(29)-ene obtained from Combretum leprosum Mart & Eich in mice.

    Science.gov (United States)

    Longhi-Balbinot, Daniela T; Lanznaster, Débora; Baggio, Cristiane Hatsuko; Silva, Morgana Duarte; Cabrera, Celeste Heiseke; Facundo, Valdir A; Santos, Adair R S

    2012-06-26

    The 3β, 6β, 16β-trihydroxylup-20(29)-ene (TTHL) is a pentacyclic triterpene obtained from a medicinal plant named Combretum leprosum. In folk medicine, this plant is used to treat several diseases associated with inflammation and pain. We previously demonstrated that TTHL presents a significant antinociceptive effect, suggesting the involvement of the glutamatergic system. This study was designed to investigate the effect of TTHL on nociception and vascular permeability induced by acetic acid. We also evaluated the effect of TTHL on carrageenan-induced peritonitis and the levels of cytokines (interleukin 1-β [IL-1β], tumor necrosis factor α [TNF-α] and interleukin 10 [IL-10]) on peritoneal fluid. TTHL was administered orally by intra-gastric gavage (i.g.) 60 min prior to experimentation. Abdominal contractions and vascular permeability were induced by an intraperitoneal (i.p.) injection of acetic acid (0.6%). We also investigated whether TTHL decreases carrageenan-induced peritonitis (750 μg/cavity) by measuring leukocyte migration and vascular permeability. In addition, we evaluated the effects of TTHL on TNF-α, IL-1β and IL-10 release induced by carrageenan on peritoneal fluid. The levels of these cytokines were measured by ELISA. TTHL (0.01-10 mg/kg) administered by intra-gastric (i.g.) gavage inhibited (69±3%) acetic acid-induced abdominal constrictions, with an ID₅₀ of 0.15 (0.03-0.8) mg/kg. TTHL (10mg/kg) also reduced the leukocyte infiltration induced by acetic acid, with an inhibition of 59±9 but had no effect on abdominal vascular permeability. In addition, indomethacin (10 mg/kg, i.p.) reduced the nociceptive behavior (92±1%), total leukocyte migration (29±3%) and capillary permeability (71±3%) induced by acetic acid. While the glucocorticoid dexamethasone (2 mg/kg, s.c.) reduced partially but significantly the nociception (31±1%), besides to promote a marked reduction on total leukocyte migration (60±2%) to the peritoneal cavity

  8. Transformations of the molecular complex of hydroiodide of 7-methyl-1,5,7-triazabicyclo[4.4.0]dec-5-ene and 5,5‧-dibromo-2,2‧-biphenol between crystal and solutions

    Science.gov (United States)

    Wojciechowski, Grzegorz; Ratajczak-Sitarz, Małgorzata; Katrusiak, Andrzej; Brzezinski, Bogumil

    2005-05-01

    A complex of 5,5'-dibromo-2,2'-biphenol with 7-methyl-1,5,7-triazabicyclo[4.4.0]dec-5-ene hydroiodide has been studied by X-ray diffraction, FT-IR, and 1HNMR spectroscopy. In the crystal structure, the iodide anion is hydrogen-bonded to the MTBDH + cation and to two hydroxyl groups of two 5,5'-dibromo-2,2'-biphenol molecules. In chloroform and acetonitrile the structure of the complex assumes a new structure due to almost complete dissociation of the protonated MTBD molecule. In chloroform the MTBDH + cation is hydrogen bonded to 5,5'-dibromo-2,2'-biphenol whereas in acetonitrile it is free and solvated by the solvent. In both cases the 5,5'-dibromo-2,2'-biphenol molecule is hydrogen bonded to the iodide anion. In chloroform the iodide ion interacts strongly with the solvent.

  9. Synthesis and Evaluation of in Vitro Biological Activity of 4-Substituted Arylpiperazine Derivatives of 1,7,8,9-Tetrachloro-10,10-dimethoxy-4-azatricyclo[5.2.1.02,6]dec-8-ene-3,5-dione

    Directory of Open Access Journals (Sweden)

    David Collu

    2009-12-01

    Full Text Available A series of twenty arylpiperazine derivatives of 1,7,8,9-tetrachloro-10,10-dimethoxy-4-azatricyclo[5.2.1.02,6]dec-8-ene-3,5-dione have been prepared. These derivatives were tested in vitro with the aim of identifying novel lead compounds active against emergent and re-emergent human and cattle infectious diseases (AIDS, hepatitis B and C, tuberculosis, bovine viral diarrhea. In particular, these compounds were evaluated in vitro against representatives of different virus classes, such as a HIV-1 (Retrovirus, a HBV (Hepadnavirus and the single-stranded RNA+ viruses Yellow fever virus (YFV and Bovine viral diarrhea virus (BVDV, both belonging to the Flaviridae. Compounds 2c, 2g and 3d showed a modest activity against CVB-2. The molecular structures of the starting imide 1 and one of propyl-piperazine derivatives, 3b, have been determined by an X-ray crystallography study.

  10. Rhodium-catalyzed [5 + 2 + 1] cycloaddition of ene-vinylcyclopropanes and CO: reaction design, development, application in natural product synthesis, and inspiration for developing new reactions for synthesis of eight-membered carbocycles.

    Science.gov (United States)

    Wang, Yi; Yu, Zhi-Xiang

    2015-08-18

    Practical syntheses of natural products and their analogues with eight-membered carbocyclic skeletons are important for medicinal and biological investigations. However, methods and strategies to construct the eight-membered carbocycles are limited. Therefore, developing new methods to synthesize the eight-membered carbocycles is highly desired. In this Account, we describe our development of three rhodium-catalyzed cycloadditions for the construction of the eight-membered carbocycles, which have great potential in addressing the challenges in the synthesis of medium-sized ring systems. The first reaction described in this Account is our computationally designed rhodium-catalyzed two-component [5 + 2 + 1] cycloaddition of ene-vinylcyclopropanes (ene-VCPs) and CO for the diastereoselective construction of bi- and tricyclic cyclooctenones. The design of this reaction is based on the hypothesis that the C(sp(3))-C(sp(3)) reductive elimination of the eight-membered rhodacycle intermediate generated from the rhodium-catalyzed cyclopropane cleavage and alkene insertion, giving Wender's [5 + 2] cycloadduct, is not easy. Under CO atmosphere, CO insertion may occur rapidly, converting the eight-membered rhodacycle into a nine-membered rhodacycle, which then undergoes an easy C(sp(2))-C(sp(3)) reductive elimination process and furnishes the [5 + 2 + 1] product. This hypothesis was supported by our preliminary DFT studies and also served as inspiration for the development of two [7 + 1] cycloadditions: the [7 + 1] cycloaddition of buta-1,3-dienylcyclopropanes (BDCPs) and CO for the construction of cyclooctadienones, and the benzo/[7 + 1] cycloaddition of cyclopropyl-benzocyclobutenes (CP-BCBs) and CO to synthesize the benzocyclooctenones. The efficiency of these rhodium-catalyzed cycloadditions can be revealed by the application in natural product synthesis. Two eight-membered ring-containing natural products, (±)-asterisca-3(15),6-diene and (+)-asteriscanolide, have been

  11. Three closely related (2E,2'E)-3,3'-(1,4-phenyl-ene)bis-[1-(meth-oxy-phen-yl)prop-2-en-1-ones]: supra-molecular assemblies in one dimension mediated by hydrogen bonding and C-H⋯π inter-actions.

    Science.gov (United States)

    Sim, Aijia; Chidan Kumar, C S; Kwong, Huey Chong; Then, Li Yee; Win, Yip-Foo; Quah, Ching Kheng; Naveen, S; Chandraju, S; Lokanath, N K; Warad, Ismail

    2017-06-01

    In the title compounds, (2E,2'E)-3,3'-(1,4-phenyl-ene)bis-[1-(2-meth-oxy-phen-yl)prop-2-en-1-one], C26H22O4 (I), (2E,2'E)-3,3'-(1,4-phenyl-ene)bis-[1-(3-meth-oxy-phen-yl)prop-2-en-1-one], C26H22O4 (II) and (2E,2'E)-3,3'-(1,4-phenyl-ene)bis-[1-(3,4-di-meth-oxy-phen-yl)prop-2-en-1-one], C28H26O6 (III), the asymmetric unit consists of a half-mol-ecule, completed by crystallographic inversion symmetry. The dihedral angles between the central and terminal benzene rings are 56.98 (8), 7.74 (7) and 7.73 (7)° for (I), (II) and (III), respectively. In the crystal of (I), mol-ecules are linked by pairs of C-H⋯π inter-actions into chains running parallel to [101]. The packing for (II) and (III), features inversion dimers linked by pairs of C-H⋯O hydrogen bonds, forming R22(16) and R22(14) ring motifs, respectively, as parts of [201] and [101] chains, respectively.

  12. MENINGKATKAN KEMAMPUAN SERVICE DALAM PERMAINAN BOLA VOLLY DENGAN MAKE A MATCH PADA SISWA KELAS VII-H SMP NEGERI 1 WONOASRI

    Directory of Open Access Journals (Sweden)

    Anik Sri Handayani

    2016-11-01

    Full Text Available In the learning process of Physical Education in, teachers are expected to teach varieties of basic motor skills, techniques and strategy of games and sports, internalization of values (sportsmanship, honesty, cooperation, etc. as well as the conditioning of healthy lifestyles. The implementation is not through conventional teaching in the classroom consisting theoretical studies, but it also involves elements of physical, mental, intellectual, emotional and social. The activities which are given in teaching should get a methodical didactic touch, so that the activities carried out can achieve the aim of teaching. The problem formulation of this research is: is there an increase in the ability of service in the game of volleyball with “make-match” at VII-H grade of SMP Negeri 1 Wonoasri in 2012/2013 academic year? The purpose Classroom Action Research (CAR is to improve the quality of learning on the physical education in doing “service” in a volleyball game through a “make-match” learning approach  In this study, researchers have collaboration with other teachers and the principal. Researchers are involved in the study from the beginning to the end of the researech. Researchers try to see, observe, feel, live, reflect and evaluate learning activities that take place. The implementation stages of the action research consist of planning, acting, observation, and reflection. To obtain accurate results, the collected data in study were statistically analyzed using the formulas of mean or average.  The result showed that the average value of the first cycle of 66.14 (46.33% while the Cycle II 91.29 (96.43% in the second cycle, it has increased significantly. Referring to the hypothesis that the actions proposed in this class action research, it can be concluded that: there is an increased ability of “service” in volleyball games with “make-a-match” at VII-H grade of SMP Negeri 1 Wonoasri in 2012/2013 academic year.   Keywords: Skill Upgrading, Make A-Match

  13. Cycloartan-24-ene-1α,2α,3β-triol, a cycloartane-type triterpenoid from the resinous exudates of Commiphora myrrha, induces apoptosis in human prostatic cancer PC-3 cells.

    Science.gov (United States)

    Gao, Wenyan; Su, Xiaojie; Dong, Xiaoyan; Chen, Yingli; Zhou, Chunlan; Xin, Ping; Yu, Chunhao; Wei, Taiming

    2015-03-01

    Plant-derived antitumor drugs are currently used in chemotherapy. Cycloartane triterpenoids have shown a cytotoxic effect on human prostate cancer cells. The aim of the present study was to isolate a cycloartane triterpenoid from Commiphora myrrha and evaluate its anticancer potential. Cycloartan-24-ene-1α,2α,3β-triol (MY-1) was isolated from Commiphora myrrha, and its structure was determined through 1H and 13C nuclear magnetic resonance spectroscopy. The cytotoxic and apoptotic effects of MY-1 on human prostatic cancer PC-3 cells were estimated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometric analysis and terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) staining assay, and the expression of apoptotic-related proteins were evaluated by western blotting. MY-1 showed cytotoxic activity on PC-3 cells in a concentration-dependent manner with an IC50 value of 9.6 µM at 24 h. MY-1 induced cell cycle arrest and apoptosis. Western blot analysis revealed that MY-1 regulated the expression levels of B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), p53 and caspase-3 in the PC-3 cells. These findings indicate that MY-1 exerts significantly pro-apoptotic activity against human hormone-independent prostatic cancer and support MY-1 as a potential anticancer drug.

  14. Structural, spectroscopic and theoretical studies of the 2:2 complex of 5,5‧-dibromo-2,2‧-biphenol with 1,5,7-triazabicyclo[4.4.0]dec-5-ene

    Science.gov (United States)

    Wojciechowski, G.; Brzezinski, B.; Naumov, P.; Chantrapromma, S.; Ibrahim, A. R.; Fun, H.-K.; Ng, S. W.

    2001-12-01

    In the crystal of the 2:2 complex of 1,5,7-triazabicyclo[4.4.0]dec-5-ene with 5,5‧-dibromo-2,2‧-biphenol a cooperative inter-intra-molecular hydrogen-bonded system is formed. The intermolecular hydrogen bonds are relatively long [2.708(6) and 2.895(7)] and do not show large proton polarizability. The intramolecular OHO- hydrogen bond is relatively short [2.489(6)]. The structure of the complex is very well reflected in its FT-IR spectrum in the solid. On the basis of the FT-IR and 1H NMR studies of the complex in chloroform and in acetonitrile a structure different than in the solid is proposed. DFT structures of both isolated ions in their ground electronic states at the B3LYP/6-31++G(d,p) level are employed to follow the effects of the hydrogen bonding on the intrinsic ionic structures.

  15. Crystal and molecular structure of a complex formed between the hydrochloride of 7-methyl-1,5,7-triazabicyclo[4.4.0]dec-5-ene and 5,5‧-dibromo-2,2‧-biphenol

    Science.gov (United States)

    Bartoszak-Adamska, E.; Wojciechowski, G.; Jaskólski, M.; Brzezinski, B.

    2001-09-01

    A complex of 5,5‧-dibromo-2,2‧-biphenol with 7-methyl-1,5,7-triazabicyclo[4.4.0]dec-5-ene (MTBD) hydrochloride has been studied using X-ray diffraction, FT-IR, and 1H NMR spectroscopy. The compound crystallises in the space group P21/c with a=9.738(2), b=12.100(2), c=18.347(4) Å,β=92.62(3)° (at 100 K), and Z=4. In the crystal structure, the MTBDH+ cation, a chloride anion, and one molecule of 5,5‧-dibromo-2,2‧-biphenol are present. In the solid state the Cl- anion is an acceptor in N+-H⋯Cl-, O-H⋯Cl- and C-H⋯Cl- intermolecular hydrogen bonds. In chloroform the structure of the complex is comparable with that in the solid, whereas in acetonitryle the complex assumes a new structure owing to the almost complete dissociation of the protonated MTBD molecule. The 5,5‧-dibromo-2,2‧-biphenol molecule is hydrogen-bonded with the chloride anion. Within this complex the OH⋯OH hydrogen bond shows very large proton polarisability indicated by the continuous absorption in the FTIR spectrum.

  16. Molecular structure of a complex formed between hydrobromide of 7-methyl-1,5,7-triazabicyclo[4.4.0]dec-5-ene and 5,5'-dibromo-2,2'-biphenol in crystal and solutions

    Science.gov (United States)

    Wojciechowski, Grzegorz; Ratajczak-Sitarz, Małgorzata; Katrusiak, Andrzej; Brzezinski, Bogumil

    2002-08-01

    A complex of 5,5'-dibromo-2,2'-biphenol with 7-methyl-1,5,7-triazabicyclo[4.4.0]dec-5-ene hydrobromide has been studied using X-ray diffraction, FT-IR, and 1H NMR spectroscopy. In the crystal structure, a bromide anion is hydrogen-bonded with MTBDH + cation and two hydroxyl groups of two 5,5'-dibromo-2,2'-biphenol molecules. In the solid state, the Br - anion is an acceptor in N +-H⋯Br -, and two O-H⋯Br - intermolecular hydrogen bonds. In chloroform and acetonitrile, the structure of the complex assumes a new structure due to almost complete dissociation of protonated MTBD molecule. In chloroform, the MTBDH + cation is hydrogen-bonded with 5,5'-dibromo-2,2'-biphenol whereas in acetonitrile it is free and solvated by the solvent. In both cases, the 5,5'-dibromo-2,2'-biphenol molecule is hydrogen-bonded to the bromide anion.

  17. Cooperative hydrogen-bonded chain in molecular ionic complex of 5,5'-dibromo-2,2'-biphenol with 7-methyl-1,5,7-triazabicyclo[4.4.0]dec-5-ene hydrofluoride

    Science.gov (United States)

    Wojciechowski, Grzegorz; Ratajczak-Sitarz, Małgorzata; Katrusiak, Andrzej; Brzezinski, Bogumil

    2002-08-01

    A complex of 5,5'-dibromo-2,2'-biphenol (DBBPh) with 7-methyl-1,5,7-triazabicyclo[4.4.0]dec-5-ene (MTBD) hydrofluoride has been studied using X-ray diffraction, FT-IR, and 1H NMR spectroscopy. The formula unit of the crystal structure consists of a MTBDH + cation, a fluoride anion, and one neutral molecule of 5,5'-dibromo-2,2'-biphenol. In the solid state the F - anion is an acceptor in two very short O-H⋯F - intermolecular hydrogen bonds of 2.487 and 2.390 Å involving the hydroxyl groups. One oxygen atom of these groups forms also an N +-H⋯O intermolecular hydrogen bond of 2.775 Å with the MTBDH + cation. In chloroform and acetonitrile, the structures of the complexes are comparable with those studied previously for 5,5'-dibromo-2,2'-biphenol with MTBD, because the hydrogen fluoride goes into the gas phase during the solution process.

  18. Antimicrobial Effect of the Triterpene 3β,6β,16β-Trihydroxylup-20(29-ene on Planktonic Cells and Biofilms from Gram Positive and Gram Negative Bacteria

    Directory of Open Access Journals (Sweden)

    Francisco Flávio Vasconcelos Evaristo

    2014-01-01

    Full Text Available This study evaluated the antimicrobial effect of 3β,6β,16β-trihydroxylup-20(29-ene (CLF1, a triterpene isolated from Combretum leprosum Mart., in inhibiting the planktonic growth and biofilms of Gram positive bacteria Streptococcus mutans and S. mitis. The antimicrobial activity was assessed by determining the minimum inhibitory concentration (MIC and minimum bactericidal concentration (MBC. The antibiofilm potential was determined by quantifying total biomass and enumerating biofilm-entrapped viable bacteria. In addition, the acute toxicity of CLF1 on Artemia sp. nauplii was also determined. The results showed that CLF1 was able in inhibiting the growth of S. mutans and S. mitis with MIC and MBC of 7.8 μg/mL and 15.6 μg/mL, respectively. CLF1 was highly effective on biofilms of both bacteria. Only 7.8 μg/mL CLF1 was enough to inhibit by 97% and 90% biomass production of S. mutans and S. mitis, respectively. On the other hand, such effects were not evident on Gram negative Pseudomonas aeruginosa and Klebsiella oxytoca. The toxicity tests showed that the LC50 of CLF1 was 98.19 μg/mL. Therefore, CLF1 isolated from C. leprosum may constitute an important natural agent for the development of new therapies for caries and other infectious diseases caused by S. mutans and S. mitis.

  19. Molecular structure, vibrational spectroscopic, first order hyperpolarizability and HOMO-LUMO studies of 7-amino-8-oxo-3-vinyl-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid.

    Science.gov (United States)

    Ramalingam, M; Sethuraman, V; Sundaraganesan, N

    2011-02-01

    The FT-IR and FT-Raman spectra of 7-amino-8-oxo-3-vinyl-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid (7AVCA) were recorded in the region 4000-400 cm(-1) and 3500-10 cm(-1), respectively. Quantum chemical calculations of energies, geometrical structure and vibrational wavenumbers were carried out by ab initio HF and density functional theoretical methods invoking 6-311G(d,p) basis set. The differences between the observed and scaled wavenumber values of most of the fundamentals are very small. The electric dipole moment (μ) and the first order hyperpolarizability (β0) values have been computed quantum mechanically. The calculated results show that 7AVCA may have microscopic nonlinear optical (NLO) behavior with non-zero values. A detailed interpretation of the FT-IR and FT-Raman spectra of 7AVCA is reported. The theoretical IR and Raman spectra of 7AVCA have also been constructed. The calculated HOMO and LUMO energies show that the charge transfer occurs within the molecule. Copyright © 2010 Elsevier B.V. All rights reserved.

  20. Zinc(II PVC-based membrane sensor based on 5,6-benzo-4,7,13,16,21,24- hexaoxa-1,10-diazabicyclo[8,8,8]hexacos-5-ene

    Directory of Open Access Journals (Sweden)

    Zamani Hassan Ali

    2006-01-01

    Full Text Available The 5,6-benzo-4,7,13,16,21,24-hexaoxa-1,10-diazabicyclo[8,8,8]hexacos-5-ene (BHDE was used as an excellent ionophore in construction of a Zn(II PVC-based membrane sensor. The best performance was obtained with a membrane composition of 30% poly(vinyl chloride, 64.5% nitrobenzen (NB, 2.5% BHDE and 3% sodium tetraphenylborate (NaTPB. This sensor shows very good selectivity and sensitivity towards zinc ion over a wide variety of cations, including alkali, alkaline earth, transition and heavy metal ions. The sensor revealed a great enhancement in selectivity coefficients for zinc ions, in comparison to the previously reported zinc sensors. The proposed sensor exhibits a Nernstian behavior (with slope of 29.1 ? 0.4 mV per decade over a wide concentration range (1.0 10-6-1.0 10-1 mol L-1 with a detection limit of 6.3 x10-7 mol L-1 (41.2 ng mL-1. It shows relatively fast response time, in the whole concentration range (< 10s, and can be used for at least 10 weeks in a pH range of 2.8-7.3. The proposed sensor was successfully used in direct determination of zinc ions in wastewater of industrial zinc electroplating companies, and also as an indicator electrode in titration with EDTA.

  1. New optically active and thermally stable poly(amide-imide)s containing N,N'-(Bicyclo[2,2,2]oct-7-ene-2,3,5,6-tetracarboxylic)-bis-L-alanine and aromatic diamines: synthesis and characterization

    Energy Technology Data Exchange (ETDEWEB)

    Faghihi, Khalil; Absalar, Morteza; Hajibeygi, Mohsen [Arak University (Iran, Islamic Republic of). Faculty of Science. Organic Polymer Chemistry Research Lab.

    2009-07-01

    Five new optically active poly(amide-imide)s (PAIs) 6a-e were prepared by direct polycondensation reaction of the newly synthesized N,N'-(bicyclo[2,2,2]oct-7-ene-2,3,5,6-tetra carboxylic)-bis-L-alanine 4 with various aromatic diamines 5a-e using polar aprotic solvents such as N-methyl-2-pyrrolidone (NMP). In this technique triphenyl phosphite (TPP) and pyridine were used as condensing agents to form poly(amide-imide)s through the N-phosphonium salts of pyridine. All of the polymers were obtained in quantitative yields with inherent viscosities between 0.29-0.46 dL g{sup -1} and were highly soluble in polar aprotic solvents such as N,N-dimethyl acetamide (DMAc), N,N-dimethyl formamide (DMF), dimethyl sulfoxide (DMSO), N-methyl- 2-pyrrolidone (NMP) and solvents such as sulfuric acid. They were fully characterized by means of {sup 1}H NMR, FTIR spectroscopy, elemental analyses, inherent viscosity, solubility test, specific rotation and thermal properties of these polymers were investigated using thermogravimetric analysis techniques (TGA and DTG). (author)

  2. Facile one-pot synthesis of a aptamer-based organic-silica hybrid monolithic capillary column by "thiol-ene" click chemistry for detection of enantiomers of chemotherapeutic anthracyclines.

    Science.gov (United States)

    Jiang, Han-Peng; Zhu, Jiu-Xia; Peng, Chunyan; Gao, Jiajia; Zheng, Fang; Xiao, Yu-Xiu; Feng, Yu-Qi; Yuan, Bi-Feng

    2014-10-07

    In the current study, we developed a facile strategy for the one-pot synthesis of an aptamer-based organic-silica hybrid monolithic capillary column. A 5'-SH-modified aptamer, specifically targeting doxorubicin, was covalently modified in the hybrid silica monolithic column by a sol-gel method combined with "thiol-ene" click reaction. The prepared monolithic column had good stability and permeability, large specific surface, and showed excellent selectivity towards chemotherapeutic anthracyclines of doxorubicin and epirubicin. In addition, the enantiomers of doxorubicin and epirubicin can be easily separated by aptamer-based affinity monolithic capillary liquid chromatography. Furthermore, doxorubicin and epirubicin spiked in serum and urine were also successfully determined, which suggested that the complex biological matrix had a negligible effect on the detection of doxorubicin and epirubicin. Finally, we quantified the concentration of epirubicin in the serum of breast cancer patients treated with epirubicin by intravenous injection. The developed analytical method is cost-effective and rapid, and biological samples can be directly analyzed without any tedious sample pretreatment, which is extremely useful for monitoring medicines in serum and urine for pharmacokinetic studies.

  3. Ajalookirjanduse aastapreemia pälvis Aivar Jürgenson / Mart Kalm

    Index Scriptorium Estoniae

    Kalm, Mart, 1961-

    2012-01-01

    Ajalookirjanduse aastapreemiale kandideeris 15 teost. Žürii nägi preemiakandidaadina teaduslikku monograafiat, otsustades anda preemia elus autorile ja vältida juba preemiad saanud teoseid. Leiti, et statuuti tuleks täpsustada. Preemia pälvis Aivar Jürgensoni teos "Ladina rahva seas. Argentina ja sealsed eestlased"

  4. Preliminary Archaeological investigation in front of Anija Manor House / Anneli Kalm, Kahrut Eller, Guido Toos

    Index Scriptorium Estoniae

    Kalm, Anneli

    2013-01-01

    2012. aastal avastati Anija mõisa peahoone ees müürikatked. Suurimad leitud rajatised olid kolm loode-kagu suunalist müüri. Profiilides sai eristada vähemalt kahte eriaegset rusude ladestumist, millest alumine sisaldas ka põlemistunnuseid. Kivirajatisega otseselt seostatavaid leide ei saadud, seega võib vaid kirjalikele allikatele toetudes oletada, et mõisahoone rajati Anijale 14. või 15. sajandil

  5. Keskklassi maailm võtab ilmet - 1920. aastate arhitektuurist / Mart Kalm

    Index Scriptorium Estoniae

    Kalm, Mart, 1961-

    2010-01-01

    Riigikoguhoonest. Sotsiaalehitus. Linnamajade ehitamine. Tööandja elamud. Asunikutalud. Spekulatiivne elamuehitus. Tallinna maja. Avalikud hooned. Puit traditsionalismi ja akademismi vahel. Importarhitektuur

  6. Mis peaks Eestist olema maailmapärandis? / Urve Sinijärv, Mart Kalm

    Index Scriptorium Estoniae

    Sinijärv, Urve, 1969-

    2011-01-01

    UNESCO maailmapärandi nimekirjast, selle täiendamisest uute objektidega. Eestist võiksid nimekirja kuuluda puisniidud, Balti klint, Kuressaare vanalinn, Narva linnus, Tartu Ülikooli ansambel, vesilennukite angaarid

  7. Paula Delacherie-Ilves (7. 04. 1903 - 2. 09. 2003) : In Memoriam / Mart Kalm

    Index Scriptorium Estoniae

    Kalm, Mart, 1961-

    2003-01-01

    Pariisis elanud eesti esimese naisarhitekti elust ja loomingust. 1933. a. abiellus arhitekt Jacques Delacheriega. 2 ill.: arhitekti portreefoto, Tallinna Keskhaigla võistlustöö "Tervis", 1936-1937 (klaasnegatiivid Eesti Arhitektuurimuseumis)

  8. Eesti majandid rahvusvahelises börsiettevõttes / Ülo Kalm

    Index Scriptorium Estoniae

    Kalm, Ülo, 1935-

    2008-01-01

    Artiklis tutvustatakse AS-i Agro Piim (AS moodustavad AS Peetri Põld ja Piim, Vaimastvere Agro Jõgevamaalt, Tammsaare OÜ ja Aravete Agro koos Arme Turvasega Järvamaalt, Lelle POÜ Raplamaalt ja Hiiumaa Agro - omanikuks Venemaa AO Nutritec Grupp) ajalugu, tegevust, perspektiive, antakse hinnang Eesti põllumajanduspoliitikale

  9. Enne tuleb tasuvus selgeks teha: [biogaasitehase rajamisest] / Ülo Kalm

    Index Scriptorium Estoniae

    Kalm, Ülo, 1935-

    2008-01-01

    Eesti suurimas seakasvatusettevõttes Eksekos, kus paikneb 41% vabariigi seakasvatusest ja tekib aastas 120 000 tonni seasõnnikut, on reaalne võimalus ehitada sõnnikust ja muudest biolagunevatest jäätmetest biogaasi tootev tehas. Skeem: Biogaasist elektri ja soojuse tootmine

  10. Vello Asi - Eesti sisearhitektuuri Grand Old Man / Vello Asi ; interv. Mart Kalm

    Index Scriptorium Estoniae

    Asi, Vello, 1927-

    2002-01-01

    Intervjuu sisearhitekti 75. sünnipäeva puhul. V. Asi õpingutest, tööst Estonprojektis, Kunstitoodete Kombinaadis, sisearhitektuuri kateedris, oma töödest, koostööst Valve Pormeistri ja Väino Tammega, eesti sisekujundusest 1960.-1970. aastail. 12 ill.

  11. Ühispanga hoone - Eesti edu tähis / Mart Kalm

    Index Scriptorium Estoniae

    Kalm, Mart, 1961-

    1999-01-01

    Tallinn, Tartu maantee 13. Pangahoone projekteerija Arhitektibüroo R. Puusepp, arhitekt Raivo Puusepp. Sisekujundaja AS PAG, sisearhitekt Erik Olle. Ehitas Merko Ehitus. Projekt 1995-98, hoone valmis 1999. Pilvelõhkujate uus põlvkond. Hoone tehnilisi näitajaid. 15 illustratsiooni

  12. Eesti tulevik on noor pere / Lennart Meri ; intervjueerinud Ülo Kalm

    Index Scriptorium Estoniae

    Meri, Lennart, 1929-2006

    1999-01-01

    Kohtumine peaminister Mart Laari valitsuse liikmetega Paslepas, 7. juuli 1999. Ilmunud ka: Vaba Eesti Sõna (New York), 2. sept., lk. 7, pealk.: President Lennart Meri: young estonian family is the future of Estonia: [lüh.

  13. Uus väravahoone : uued hooned ja rajatised / Mart Kalm, Märt Karmo

    Index Scriptorium Estoniae

    Kalm, Mart, 1961-

    2004-01-01

    1998. a. arhitektuurivõistlusest, kus esikoha pälvis Aivo Schultsi ja Marti Kahu võistlusprojekt "Vihm". 2003. a. hakkas hoonet ehitama AS Merko Ehitus. Sisearhitekt Priit Põldme. Novembris 2003 valminud hoonest, muudatustest. Ill.: 5 konkursitöö joonised, valminud hoone välis- ja sisevaade

  14. Oma niši leidmine : [Kiili valla aiandustalust] / Ülo Kalm

    Index Scriptorium Estoniae

    Kalm, Ülo, 1935-

    2004-01-01

    Aiandussaaduste sortimenti oluliselt laiendanud talunik Aivar Somarokov köögiviljade, lillede ja maitsetaimede kasvatamisest Kiili vallas asuvas Sepa talus, toodangu turustamisest ja tulevikuplaanidest

  15. Kui mälestada, siis väärikalt / Mart Kalm

    Index Scriptorium Estoniae

    Kalm, Mart, 1961-

    2008-01-01

    Tallinnasse kavandatavat diletantlikku monumenti võrreldakse professionaalsete lahendustega linnaruumis - vabadusmonumendiga Pärnus Rüütli platsil (Mart Aas, Kaarel Eelma, Mikk Mutso) ja Narva Kolledzhi projektiga (Indrek Peil, Siiri Vallner, Katrin Koov) hävinud börsihoone mälestamiseks

  16. Kohustus kannatada, et näha ja mõista. Ene Mihkelsoni ja Ingeborg Bachmanni kirjanikunägemus. The Writer’s Duty to Suffer in Order to See and Understand: The Literary Vision of Ene Mihkelson and Ingeborg Bachmann

    Directory of Open Access Journals (Sweden)

    Aija Sakova

    2012-04-01

    Full Text Available In an interview from 1971 the Austrian poet and author Ingeborg Bachmann (b 1923 said that there are phenomena that science and scientific language cannot explain. According to her these are the phenomena that belong to the realm of writers and literature. In the same year Ene Mihkelson (b 1946, who had not yet published a single book of poetry or a novel because of censorship, pondered on the role of the author within society: “The author digs under the surface and the author’s responsibility to society lies in his/her persistence, in his/her duty to suffer, to endure stress, which the people in the midst of their busy, daily lives are unable to fully grasp.” Responsibility to society and the duty to suffer and explore the deepest layers below the surface are common to both Mihkelson and Bachmann. Both writers were born and grew up in the shadow of war, violence and totalitarianism. Mihkelson was brought up in a guerrilla family in Soviet Estonia; Bachmann spent her childhood years growing up in the Nationalist-Socialist Austria and in her youth she witnessed the Second World War and its catastrophic consequences. So both of them understand that as writers they cannot overlook the events that they had, either directly or indirectly, witnessed. In an interview from 2009 Mihkelson said that the fact that she remained alive while many did not, that she was not deported to Siberia although it could have happened, has obligated her to write and reflect on what had happened. No consensus has been reached in Europe to condemn Communism as a crime against humanity, but for Estonians Communism and the 50 years of Soviet regime signify a lengthy experience of a totalitarian regime and, in spite of a possible opposition from German-speaking cultures, Communism very often equals Nazism in Estonia. According to Mihkelson, in order to understand and reflect this experience it is not sufficient to rely only on the archives, biographies and books of

  17. The roles of pregn-5-ene-3β,20α-diol and 20α-hydroxy steroid dehydrogenase in the control of progesterone synthesis preceding parturition and lactogenesis in the rat

    Science.gov (United States)

    Kuhn, N. J.; Briley, M. S.

    1970-01-01

    1. The activity of 20α-hydroxy steroid dehydrogenase in rat ovarian corpora lutea increased at least 50-fold between 2 days before and 2 days after parturition, and then fell gradually during lactation. The activity of 3β-hydroxy Δ5-steroid dehydrogenase decreased by 50% at parturition but remained constant at other times. 2. The 20α-hydroxypregn-4-en-3-one/progesterone concentration ratio in the ovary fell tenfold between 1 day before and 1 day after parturition, in contrast with the increase of the ratio for these steroids in plasma. 3. Pregnenolone was metabolized in intact cells or cell-free systems either to pregn-5-ene-3β,20α-diol and then to 20α-hydroxypregn-4-en-3-one by 20α-hydroxy steroid dehydrogenase and 3β-hydroxy Δ5-steroid dehydrogenase respectively, or directly to progesterone by the latter enzyme. The relative activities of these pathways appeared to reflect the relative amounts of the two enzymes and the concentrations of their respective coenzymes NADPH and NAD+. 4. From these and other observations it was concluded that the cessation of progesterone secretion, which precedes parturition and lactogenesis at the end of pregnancy, is partly due to the redirected metabolism of pregnenolone away from progesterone and towards 20α-hydroxypregn-4-en-3-one as the secreted end product. This is primarily the consequence of the sharp increase in the activity of 20α-hydroxy steroid dehydrogenase. This mechanism is super-imposed on the already declining rate of net Δ4-steroid release by the ovary. 5. A relationship of these pathways to subcellular compartments of luteal cells is proposed. PMID:4392955

  18. Situación de riesgo y salud de los adolescentes en el valle de los ríos Apurímac, Ene y Mantaro en Perú

    Directory of Open Access Journals (Sweden)

    Fabiola Yeckting Vilela

    Full Text Available Se presenta el diagnóstico de las condiciones de vida de los adolescentes varones y mujeres en la zona del valle de los ríos Apurímac, Ene y Mantaro (VRAEM, quienes atraviesan situaciones de riesgo y vulnerabilidad social y sexual debido a los altos niveles de producción de la hoja de coca y el contexto del narcotráfico, que crean la ilusión del rápido ascenso social. A partir de ello, es necesario preguntarse ¿cuáles son las condiciones de vida y situaciones de riesgo de los adolescentes en el VRAEM? y ¿cuáles son las alternativas para una mejora de su situación desde el acceso a la educación y los programas de atención de su salud? Esta situación de riesgo es el resultado de las relaciones desiguales de género, violencia sexual, deserción escolar, embarazo adolescente, consumo de alcohol y drogas, así como de la falta de oportunidades como consecuencia de la participación en la economía de la coca y el narcotráfico entre los adolescentes en el VRAEM. Finalmente, se presentan los resultados de la atención de profesionales de los centros de salud para favorecer la consejería en salud sexual y reproductiva y el acceso a los servicios de salud del Programa Vida Adolescente, como oportunidad de vincular los contenidos del currículo de educación y las metas de cumplimiento de este sector, visibilizando la necesidad de un enfoque de interculturalidad e igualdad de género en el acceso a la salud y la educación de los adolescentes.

  19. 7-{[2-(4-Hydroxyphenylmethylidene]amino}-1,3-thiazol-4-yl-2-(methoxyiminoacetyl]amino}-3-{[(2-methyl-5,6-dioxo-1,2,5,6-tetrahydro-1,2,4-triazin-3-ylsulfanyl]methyl}-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic Acid

    Directory of Open Access Journals (Sweden)

    Ghulam Fareed

    2012-05-01

    Full Text Available Novel 7-{[2-(4-hydroxyphenylmethylidene]amino}-1,3-thiazol-4-yl-2-(methoxyiminoacetyl]amino}-3-{[(2-methyl-5,6-dioxo-1,2,5,6-tetrahydro-1,2,4-triazin-3-ylsulfanyl]methyl}-8-oxo-5-thia-1azabicyclo [4.2.0]oct-2-ene-2-carboxylic acid was prepared by condensation of ceftriaxone disodium (1 with 4-hydroxybenzaldehyde (2 in ethanol under reflux conditions for 3–4 h. The structure of synthesized compound was elucidated using LCMS, 1H-NMR, and CHN techniques.

  20. Aproximación a la situación de salud en tres comunidades Asháninkas ubicadas en las cuencas del río Tambo y Ene, 2006

    Directory of Open Access Journals (Sweden)

    Carolina Tarqui-Mamani

    2009-01-01

    Full Text Available En mayo de 2006 se realizó un estudio transversal en tres comunidades Asháninkas ubicadas en las cuencas de los ríos Tambo y Ene, para describir su situación de salud. Se definió la situación salud sobre la base de cinco indicadores: prevalencia de la desnutrición infantil, enfermedades diarreicas agudas (EDA, infecciones respiratorias agudas (IRA, mortalidad materna y mortalidad infantil. Se interrogó a los padres o madres de familia acerca de defunciones en menores de 1 año, defunciones maternas, casos de EDA e IRA. Se evaluó el estado nutricional mediante la medición del peso y la talla en niños de 0 a 6 años y se observó el estado de protección vacunal en los carnés de vacunación. Se realizó 53 entrevistas a los padres y se evaluó a 83 niños en las comunidades de Samaniato, Tziquireni y Mayapo. No se reportó muertes maternas ni infantiles. La desnutrición global alcanzó 43,3% y fluctúa entre 53 a 57% entre los 12 y los 47 meses y aumenta a 58,8% después de los 48 meses. La desnutrición crónica alcanzó al 78,3% de niños evaluados. Los padres reportaron que 53,1% de los niños presentaron un episodio de EDA y 57% de IRA. 98,9% de los niños mayores de un año estuvieron protegidos. Los resultados encontrados permiten una aproximación a la situación de salud en las comunidades Asháninkas, lo cual debe favorecer el diseño de programas de intervención focalizados o acciones de ayuda nutricional dirigidas a los niños de dicha comunidad.

  1. Synthesis, biological evaluation, and molecular docking studies of benzyl, alkyl and glycosyl [2-(arylamino)-4,4-dimethyl-6-oxo-cyclohex-1-ene]carbodithioates, as potential immunomodulatory and immunosuppressive agents.

    Science.gov (United States)

    El Ashry, El Sayed H; Amer, Mohammad R; Abdalla, Omer M; Aly, Aly A; Soomro, Samreen; Jabeen, Almas; Halim, Sobia Ahsan; Ahmed Mesaik, M; Ul-Haq, Zaheer

    2012-05-01

    The immunomodulating properties of functionalized [2-(arylamino)-4,4-dimethyl-6-oxo-cyclohex-1-ene] carbodithioates and 6,6-dimethyl-4-(2-(propan-2-ylidene)hydrazinyl)-6,7-dihydro-2H-indazole-3(5H)-thione compounds have been investigated. Four of them, 13, 18, 19 and 20 inhibited PBMC proliferation induced by phytohemagglutinin (PHA) in a dose dependent manner with an IC(50) of ≤ 20 μM. The Th-1 cytokine, interleukin-2 (IL-2) in PHA/PMA-stimulated peripheral blood mononuclear cells (PBMCs) is significantly inhibited by 13, 19 and 20 with an IC(50) of 8.4 ± 0.4, 5.34 ± 0.15 and 4.9 ± 0.7 μM, respectively. They also inhibited the PMA/lipopolysaccharide-induced proinflammatory cytokines, IL-1β and TNF-α production in human monocytic leukemia cells (THP-1), by 86%, 46% and 59.2% for IL-1β and by 83.8%, 48.2% and 58.7% for TNF-α, respectively. Only 20 showed significant suppressive activity against the phagocyte oxidative burst in a dose dependent manner, with an IC(50) of 23.8 μM. LPS-induced nitrites in mouse macrophages were found to be inhibited by compounds 6, 8, 13-15 and 19 with an IC(50), which range between 7.7 and 63 μM. The cytotoxicity for the active compounds was also studied on Rat Wistar Hepatocyte cell line, CC1 and the Mouse Fibroblast cell line 3T3 NIH in the presence of compounds using a standard MTT assay. Furthermore, structural-activity relationship using automated docking software revealed that active compounds 7, 13 and 19, adapted the same binding mode, however the most active compound 20 is found deeply inserted within the ligand binding site of IL-2, as multiple hydrophobic and hydrophilic key interactions stabilize the compound inside the binding site, thus contributing higher activity. Copyright © 2012 Elsevier Ltd. All rights reserved.

  2. Vaesusriski muutused Eestis / Ene-Margit Tiit

    Index Scriptorium Estoniae

    Tiit, Ene-Margit, 1934-

    2006-01-01

    Seoses Eesti majanduse kiire arenguga tellis sotsiaalministeerium 2005. aastal uue tarbimiskaalude hinnangu, mille aluseks olid leibkonna eelarvehinnangu andmed aastaist 2000-2004 ning hinnati erineva struktuuriga leibkondade tarbimiskulutusi. Diagrammid. Graafikud

  3. Maia Laul / Maia Laul ; interv. Ene Ammer

    Index Scriptorium Estoniae

    Laul, Maia, 1921-2009

    1998-01-01

    Maia Laul eriala omandamisest, õppejõududest. Lõpetas 1949. a. Tallinna Riikliku Tarbekunsti Instituudi. Alar Kotli käealusena "Estonia" teatri taastamisprojekti juures. Peeter Tarvase ja August Volbergiga ENSV paviljponi kujundamas Moskvas VDNHs. Töötamisest ARSis. Sisearhitektuur ja monumentaalkunst (koostöö Eva Jänesega). Esinemistest näitustel "Ruum ja vorm". Ühiskondlike hoonete sisekujundustest. Vanaturu apteek (klaasigraafika Tiit Jürna)

  4. Modernne kastmaja Nõmmel / Ene Läkk

    Index Scriptorium Estoniae

    Läkk, Ene, 1957-

    1999-01-01

    Projekteerija: arhitektuuribüroo Mark & Tamm. Konstruktiivne osa: inseneribüroo Randväli Karema. Arhitektid Ülar Mark, Kalle Komissarov. Kahekorruseline maja Vabaduse pst. ääres on mahutatud puude vahele kitsale alale. Kommenteerib Ü. Mark.

  5. Szentendre koolkond / Ene Asu-Õunas

    Index Scriptorium Estoniae

    Asu-Õunas, Ene, 1943-

    2005-01-01

    Näitus "Szentendre kunstnikekoloonia 75" Tartu Kunstimajas. 1928. a. asutatud koloonia taastati 1997. astal. 12 kunstnikku saavad 1-3 aasta vältel kasutada külalisateljeed. Szentendre on tänaseks Euroopa kunstnike kolooniate võrgu liige

  6. Seadusemuudatused, mis teevad elu paremaks / Ene Kaups

    Index Scriptorium Estoniae

    Kaups, Ene, 1963-

    2008-01-01

    2008. aastal jõustuvatest seadusemuudatustest. Ilmunud ka Koit 7. veeb. 2008, lk. 6 ; Lääne Elu 7. veeb. 2008, lk. 2 ; Hiiu Leht 8. veeb. 2008, lk. 2 ; Järva Teataja 9. veeb. 2008, lk. 2 ; Türi Rahvaleht 8. veeb. 2008, lk. 4, pealkiri kujul: Seadusemuudatustest ; Meie Maa 15. veeb. 2008, lk. 2 ; Sakala 15. veeb. 2008, lk. 2, pealkiri kujul: Käesolev aasta toob hulga olulisi muudatusi ; Sõnumitooja 27. veeb. 2008, lk. 2 ; Valgamaalane 26. veeb. 2008, lk. 2

  7. Mida lugeja raamatukogult tegelikult ootab / Ene Riet

    Index Scriptorium Estoniae

    Riet, Ene, 1954-

    2011-01-01

    4. teadus- ja erialaraamatukogude päev toimus 21. oktoobril 2011 Eesti Muusika- ja Teatriakadeemias. Teadusraamatukogu aasta teo auhinna pälvis Urmas Sinisalu tulemusliku ja innovaatilise töö eest raamatukogude infosüsteemi ESTER arendamisel. Erialaraamatukogu aasta teo auhinna sai TÜ ajakirjanduse ja kommunikatsiooni instituudi raamatukoguhoidja Maio Vaniko raamatukogundust arendava tegevuse eest

  8. Riik arhitektuuritellijana / Ene Läkk

    Index Scriptorium Estoniae

    Läkk, Ene, 1957-

    2010-01-01

    Näidete varal Riigi Kinnisvara AS-i korraldatud projekteerimise hangetest, mil välja valiti odavaim pakkumine. Valminud Rakvere politseihoonest ning Jõgeva kohtu- ja politseihoonest. Mudelprojekteerimise (BIM) nõudest. Riigihankest kui salastatud protsessist. Sunnist loobuda autoriõigusest. Riigihankega ühendatud arhitektuurikonkursist

  9. 968-IJBCS-Article-Ene Pascal Chuka

    African Journals Online (AJOL)

    T60

    Keywords: Adherent E. coli (DEC); Enteropathogenic E. coli (EPEC); Enteroaggregative E. coli (EAEC);. Diffusely adherent E. coli (DAEC); Mixed Infections; Intestinal Parasites. INTRODUCTION. Among the bacterial causes of diarrhea is Escherichia coli. The diarrheagenic E. coli. (DEC) is the most important bacterial.

  10. Professor Pekka Puska loengud / Ene Indermitte

    Index Scriptorium Estoniae

    Indermitte, Ene, 1967-

    2004-01-01

    Soome Rahvatervishoiu Instituudi direktor, Põhja-Karjala projekti juht prof Pekka Puska peab Tartu ülikoolis loengu "The North Karelia Projekt in Finland and lessons for global chronic disease prevention"

  11. Cultural Trauma and Life Stories / Ene Kõresaar

    Index Scriptorium Estoniae

    Kõresaar, Ene

    2007-01-01

    Aili Aarelaid-Tarti 15-aastase uuringu tulemused raamatus "Cultural Trauma and Life Stories", Hesinki, Kikimora Publications, 2006. Uuritud on kolme suurt rahvusgruppi 1940-test tingitud trauma kontekstis: eestlased kodumaal, eestlased eksiilis ja venekeelne rahvusgrupp Eestis postsovetlikus diskursuses

  12. Küberjulgeolekule teed rajades / Ene Ergma

    Index Scriptorium Estoniae

    Ergma, Ene, 1944-

    2007-01-01

    Riigikogu esimees analüüsib aprillisündmustega kaasnenud küberrünnakute kui ühe terrorismi erivormi tagamaid, toob välja vajaduse kujundada küberrünnakute suhtes Euroopa Liidus ja NATO-s ühtne poliitika

  13. Kas 270 rahvaraamatukogust piisab? / Ene Riet

    Index Scriptorium Estoniae

    Riet, Ene, 1954-

    2016-01-01

    Haldusreformi mõjust rahvaraamatukogude võrgule. Uuringust "Era- ja avalike teenuste ruumilise paiknemise ja kättesaadavuse tagamisest ja teenuste käsitlemisest maakonnaplaneeringute käigus" (2015)

  14. Muutuv Eesti pere / Ene-Margit Tiit

    Index Scriptorium Estoniae

    Tiit, Ene-Margit, 1934-

    2008-01-01

    Ettekanne Vabariigi Presidendi akadeemilise nõukogu ja Tallinna Ülikooli korraldatud konverentsil "Jätkusuutlik Eesti" 25. mail 2006. a. Tallinnas. Euroopa perekonna muutumisest 20. sajandi esimesest poolest 21. sajandini, perekonna eluviisi ja elustiili muutumisest Eestis, tänapäeva Eesti pere elujärjest ja sellega rahulolust. Lisatud statistilised diagrammid ja joonised

  15. Alfred Vogel / Jasmiin-Ene Kallas

    Index Scriptorium Estoniae

    Kallas, Jasmiin-Ene

    1999-01-01

    Ravimtaimede tundja, rändur, toitumisteadlane, publitsist, maailmamainega lektor, ühe tuntuima firma Bioforce AG looja shveitslane Alfred Vogel (1902-1996). Järgneb 1.,8.,15.,22.,29. dets. ; 2000, 5.,12.,19. jaan.

  16. Redetermination of cyclo-trimethyl-ene-trinitramine.

    Science.gov (United States)

    Hakey, Patrick; Ouellette, Wayne; Zubieta, Jon; Korter, Timothy

    2008-07-09

    The redetermined structure of 1,3,5-trinitro-1,3,5-triaza-cyclo-hexane, C(3)H(6)N(6)O(6), at 90 (2) K has ortho-rhom-bic (Pbca) symmetry. It is of inter-est with respect to energetic compounds. The structure was originally investigated through X-ray diffraction by Hultgren [(1936). J. Chem. Phys.4, 84]. Later X-ray investigations were completed by McCrone [(1950). Anal. Chem.22, 954-955] and Harris, Reed & Gluyas [(1959). AFOSR-TR-59-165 Ohio State University Research Foundation, Columbus, Ohio, USA]. A single-crystal neutron diffraction study was performed by Choi & Prince [(1972). Acta Cryst. B28, 2857-2862] to ascertain the H-atom positions, which had not been defined by the earlier X-ray diffraction studies. All previous studies were performed at or near room temperature. The structure provided is the α polymorph of the title compound. The ring atoms are arranged in the chair conformation with two nitro groups occupying pseudo-equatorial positions and the remaining nitro group is axial. The crystal packing is stabilized by close intramolecular interactions from one H atom in each methylene group to O atoms of adjacent nitro groups, ranging from 2.251 to 2.593 Å.

  17. Uued suunad ID-maailmas / Ene Vihtla

    Index Scriptorium Estoniae

    Vihtla, Ene

    2010-01-01

    Alates 2010. aasta oktoobrist väljastab Politsei- ja Piirivalveamet digitaalset isikutunnistust ehk digi-ID. 1. jaanuarist muutuvad ID-kaartide turvamärgid ja Eestis elavatele välismaalastele, kes ei ole EL-i kodanikud, hakatakse ID-kaardi asemel välja andma elamisloakaarti

  18. Eesti Etnograafia Ring Kanadas / Ene Runge

    Index Scriptorium Estoniae

    Runge, Ene, 1919-1999

    1993-01-01

    Etnograafia Ring looodi 1971.a ja on pidevalt õpetanud käsitööd, pidanud loenguid eesti ja inglise keeles, korraldanud näitusi ja loonud eesti sektsiooni Royal Ontario Museumi ja Canadian National Museumi juurde kultuurivara säilitamiseks. Seltsi 20. aastapäevaks oli välja antud 61 õppekirja, lisaks õpetused ajakirjas "Triinu" ja õppefilmid, fotod ja diapositiivid, abi on antud samasugustele ringidele Rootsis ja Austraalias, ERM-ile saadeti 1991. a üle 200 rahvakultuuri eseme

  19. Kaitseliit vajas Naiskodukaitse toetust / Ene Puusemp

    Index Scriptorium Estoniae

    Puusemp, Ene, 1942-

    2007-01-01

    Tartu Linnamuuseumis avatud näitusest "Õed Soome lahe kahelt kaldalt", kus tutvustatakse 1921. aastal loodud Soome naisorganisatsiooni Lotta Svärd ja ja 1927. aastal Eestis moodustatud Naiskodukaitse arengulugu

  20. Pen and Pal are nucleotide-sugar dehydratases that convert UDP-GlcNAc to UDP-6-deoxy-D-GlcNAc-5,6-ene and then to UDP-4-keto-6-deoxy-L-AltNAc for CMP-pseudaminic acid synthesis in Bacillus thuringiensis.

    Science.gov (United States)

    Li, Zi; Hwang, Soyoun; Ericson, Jaime; Bowler, Kyle; Bar-Peled, Maor

    2015-01-09

    CMP-pseudaminic acid is a precursor required for the O-glycosylation of flagellin in some pathogenic Gram-negative bacteria, a process known to be critical in bacterial motility and infection. However, little is known about flagellin glycosylation in Gram-positive bacteria. Here, we identified and functionally characterized an operon, named Bti_pse, in Bacillus thuringiensis israelensis ATCC 35646, which encodes seven different enzymes that together convert UDP-GlcNAc to CMP-pseudaminic acid. In contrast, Gram-negative bacteria complete this reaction with six enzymes. The first enzyme, which we named Pen, converts UDP-d-GlcNAc to an uncommon UDP-sugar, UDP-6-deoxy-D-GlcNAc-5,6-ene. Pen contains strongly bound NADP(+) and has distinct UDP-GlcNAc 4-oxidase, 5,6-dehydratase, and 4-reductase activities. The second enzyme, which we named Pal, converts UDP-6-deoxy-D-GlcNAc-5,6-ene to UDP-4-keto-6-deoxy-L-AltNAc. Pal is NAD(+)-dependent and has distinct UDP-6-deoxy-d-GlcNAc-5,6-ene 4-oxidase, 5,6-reductase, and 5-epimerase activities. We also show here using NMR spectroscopy and mass spectrometry that in B. thuringiensis, the enzymatic product of Pen and Pal, UDP-4-keto-6-deoxy-L-AltNAc, is converted to CMP-pseudaminic acid by the sequential activities of a C4″-transaminase (Pam), a 4-N-acetyltransferase (Pdi), a UDP-hydrolase (Phy), an enzyme (Ppa) that adds phosphoenolpyruvate to form pseudaminic acid, and finally a cytidylyltransferase that condenses CTP to generate CMP-pseudaminic acid. Knowledge of the distinct dehydratase-like enzymes Pen and Pal and their role in CMP-pseudaminic acid biosynthesis in Gram-positive bacteria provides a foundation to investigate the role of pseudaminic acid and flagellin glycosylation in Bacillus and their involvement in bacterial motility and pathogenicity. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

  1. Pen and Pal Are Nucleotide-Sugar Dehydratases That Convert UDP-GlcNAc to UDP-6-Deoxy-d-GlcNAc-5,6-ene and Then to UDP-4-Keto-6-deoxy-l-AltNAc for CMP-Pseudaminic Acid Synthesis in Bacillus thuringiensis*♦

    Science.gov (United States)

    Li, Zi; Hwang, Soyoun; Ericson, Jaime; Bowler, Kyle; Bar-Peled, Maor

    2015-01-01

    CMP-pseudaminic acid is a precursor required for the O-glycosylation of flagellin in some pathogenic Gram-negative bacteria, a process known to be critical in bacterial motility and infection. However, little is known about flagellin glycosylation in Gram-positive bacteria. Here, we identified and functionally characterized an operon, named Bti_pse, in Bacillus thuringiensis israelensis ATCC 35646, which encodes seven different enzymes that together convert UDP-GlcNAc to CMP-pseudaminic acid. In contrast, Gram-negative bacteria complete this reaction with six enzymes. The first enzyme, which we named Pen, converts UDP-d-GlcNAc to an uncommon UDP-sugar, UDP-6-deoxy-d-GlcNAc-5,6-ene. Pen contains strongly bound NADP+ and has distinct UDP-GlcNAc 4-oxidase, 5,6-dehydratase, and 4-reductase activities. The second enzyme, which we named Pal, converts UDP-6-deoxy-d-GlcNAc-5,6-ene to UDP-4-keto-6-deoxy-l-AltNAc. Pal is NAD+-dependent and has distinct UDP-6-deoxy-d-GlcNAc-5,6-ene 4-oxidase, 5,6-reductase, and 5-epimerase activities. We also show here using NMR spectroscopy and mass spectrometry that in B. thuringiensis, the enzymatic product of Pen and Pal, UDP-4-keto-6-deoxy-l-AltNAc, is converted to CMP-pseudaminic acid by the sequential activities of a C4″-transaminase (Pam), a 4-N-acetyltransferase (Pdi), a UDP-hydrolase (Phy), an enzyme (Ppa) that adds phosphoenolpyruvate to form pseudaminic acid, and finally a cytidylyltransferase that condenses CTP to generate CMP-pseudaminic acid. Knowledge of the distinct dehydratase-like enzymes Pen and Pal and their role in CMP-pseudaminic acid biosynthesis in Gram-positive bacteria provides a foundation to investigate the role of pseudaminic acid and flagellin glycosylation in Bacillus and their involvement in bacterial motility and pathogenicity. PMID:25414257

  2. Pätsi pärand Kadriorus : Presidendi kantselei interjööride rekonstruktsioon / Mart Kalm

    Index Scriptorium Estoniae

    Kalm, Mart, 1961-

    2003-01-01

    2002. a. valminud sisekujunduse autor Juta Lember (Lember & Padar). Kooti valmis 1940. a. konkursi võitnud Aarne Mõttuse vapivaip. Presidendi kantselei (A. Kotli, 1937-38) algsest kujundusest (R. Wunderlich, O. Siinmaa, Adamson-Eric) ja muudatustest (L. Pärtelpoeg, M. Summatavet), Kadrioru lossist ja pargist, vahipataljoni kasarmute (R. Koolmar, 1939) lammutamisest, kunstimuuseumi ehitamisest, Lastepargi hoonest ( A. Kotli, V. Seidra, 1937). 10 ill

  3. Paneelikas trügib palee kõrvale : UNESCO maailmapärandi nimekiri uueneb / Mart Kalm

    Index Scriptorium Estoniae

    Kalm, Mart, 1961-

    2009-01-01

    UNESCO maailmapärandi nimestikust, valikuprintsiipidest, nende avardumisest, 20. sajandi arhitektuurist, uutest UNESCO maailmapärandi nimistusse lisatud ja ootejärjekorras seisvatest objektidest. Lisa: UNESCO maailmapärandi nimistusse kantud 20. sajandi arhitektuuriobjektid

  4. Talu kui suvila arhetüüp = Farmhouse as an Archetype for a Summer Cottage / Mart Kalm

    Index Scriptorium Estoniae

    Kalm, Mart, 1961-

    2002-01-01

    Arhitekt Jüri Okase kavandatud ehitusettevõtja Rein Tallermo Mardi talus Saaremaal Kihelkonna vallas Vaigu külas on säilitanud rannaküla taluhoonete (elamu, kõrvalhoone, saun) väikesed mahud endistel kohtadel. Projekt ja hooned valmisid 2002. a. Ill.: kolm plaani, viis välisvaadet.

  5. Kolm nuppu ja lint : Helsingi Pihlajamäelt Tallinna Trummi tänavale / Mart Kalm

    Index Scriptorium Estoniae

    Kalm, Mart, 1961-

    2004-01-01

    Arhitekt Raine Karbi kavandatud Teaduste Akadeemia SKB elamurühmast Tallinnas Trummi t. 21 (1969-1971). 1970. a. tellis TA arhitekt Rein Kerstenilt juurde kakas 9-korruselist tornelamut Trummi t. 23 ja 23a. Raine Karbi eeskujudest Soomes. Helsingi Pihlajamäe elamurajoonist, mis ehitati välja 1960-ndate esimesel poolel ning mille planeeringu koostas Olli Kivinen. 3 plaani, 10 fotot. Bibliograafia lk. 62

  6. Puhtus ja kord majas ning mõtetes / Ene Vool

    Index Scriptorium Estoniae

    Vool, Ene, 1948-

    2007-01-01

    Kalle Rõõmuse projekteeritud inimese bioenergiast lähtuvast eramust, kus raudse järjekindlusega kasutati energeetilisest seisukohast ülimalt inimsõbralikke loodusmaterjale - mida vähem materjale, seda vähem väljamõjusid. Sisearhitekt oli Terje Kivist. Kalle Rõõmuse kommentaar

  7. Episteemilise loogika mõningad aspektid / Ene Grauberg

    Index Scriptorium Estoniae

    Grauberg, Ene, 1944-

    2004-01-01

    Episteemiline loogika on modaalse loogika alasüsteemi loogika teooria, mis kasutab episteemilise operaatoreid ...teab,et..., ...eeldab,et.... Tema aineks on neid operaatoreid sisaldavate otsustuste õige organiseerimise reeglid ja tingimused. Varem ilmunud: Metod modelirovanija i nekotorõje filosofskije problemõ istorii i metodologii jestestvoznanija. Tallinn, 1975.

  8. Lapsevanemate osalemine koolielus ja rahulolu kooliga / Eda Heinla, Ene Pill

    Index Scriptorium Estoniae

    Heinla, Eda, 1953-

    2008-01-01

    Artikkel analüüsib lapsevanemate koostööd kooliga, hinnanguid koolitöös osalemise võimalustele, nägemusi kooli hoolekogu ülesannetest ning rahulolu lapse kooli suhtes. Artikkel põhineb Tallinna üldhariduskoolide õpilaste vanemate küsitlusel

  9. Panaches horizontaux non-Boussinesq en milieu homog\\`ene

    CERN Document Server

    Daddi-Moussa-Ider, Abdallah; Mehaddi, Rabah; Vauquelin, Olivier; Candelier, Fabien

    2014-01-01

    The environmental impact of pollutants and effluents discharged into the atmosphere or the oceans has led researchers to conduct studies related to this issue. Several works have been carried out in this context in order to reduce the effect on the local environment. These types of ejections in nature are modeled as jets in the presence of a density gradient. In this study we treated the problem of inclined round turbulent buoyant jets and plumes ejected in a homogeneous or stratified fluid, at rest or in motion. The prediction of the flow behavior, i.e. the evolution of its variables, is first treated theoretically from a model whose formalism is valid in both the Boussinesq case as well as in the non-Boussinesq general case. Solving the equations governing the plumes is performed numerically using a Runge-Kutta 4th order. To validate the model, laboratory experiments are performed with round jets of air and helium for a wide range of densities. The confrontation theory-experience aims here to fix the limits...

  10. Läänemeri vs Nord Stream / Ene Kaups

    Index Scriptorium Estoniae

    Kaups, Ene, 1963-

    2009-01-01

    Riigikogu võttis vastu avalduse Läänemerre kavandatava gaasijuhtmega seotud keskkonnariskidest, kus avaldatakse muret, et Nord Stream AG poolt algatatud gaasijuhtme projekti rahvusvahelise keskkonnamõju hinnangutes ei ole piisavalt arvestatud kõigi seonduvate keskkonnariskidega

  11. Leedu graafika Tartus / Ene Asu-Õunas

    Index Scriptorium Estoniae

    Asu-Õunas, Ene, 1943-

    1999-01-01

    Näitus "Leedu graafika iseseisvusaastatel" Kaunase rahvusliku M. K. Ciurlionise nimelise kunstimuuseumi kogudest 26. veebr.-st Tartu Kunstimuuseumis. Esindatud 21 autori teosed. Rahvusvaheliselt tuntumaid kunstnikke Aldomas Galdikas.

  12. Ain Prosa ja Surma õpipoiss / Ain Prosa ; interv. Ene Paaver

    Index Scriptorium Estoniae

    Prosa, Ain, 1967-

    2001-01-01

    Terry Pratchetti "Mort - Surma õpipoiss" Eesti Draamateatris, lavastaja Ain Prosa. Lavastajast ja tema töödest. Arvavad ka stsenarist Mihkel Ulman, näitleja Pille Lukin ja Rakvere teatri direktor Indrek Saar

  13. ŽENE I SPORT U CRNOJ GORI

    Directory of Open Access Journals (Sweden)

    Cheryl Cooky

    2011-08-01

    Full Text Available This paper is part of a larger study that identifies who plays sport in Montenegro, and the factors (family, education, economic, religious, cultural stereotypes, and so on that contribute to girls and women’s participation in sport or that limit girls and women’s participation in sport. This study is the first evidencebased research assessing the current status of girls and women’s sport participation in Montenegro, at all institutional levels. Using mixed-methodologies (quantitative assessment, survey data and qualitative focus group interviews this study will determine the numbers of girls and women participating in sport, as compared to boys and men in similar demographic categories, and to examine why girls and women do or do not participate in sport and what are their experiences in sport. Initial findings from the demographic assessment of sport in Montenegro illustrate overall gender disparities in sport participation in the 7 most popular sports; the majority of athletes, coaches, managers and decision-makers, medical staff, referees and sport delegates were men. We found the greatest gender imbalance in soccer and basketball, which were overwhelmingly male-dominated, and the greatest gender parity in handball and volleyball- and in some cases there were more female participants than male participants in these sports. We also examined overall trends in gender and sport participation across the different geographical regions, which were similar. However, there were differences in the types of sports men and women played in the different regions, which may indicate that some sports were more popular, or accepted, or easier to access for women in the central part of the country while other sports were more popular, or accepted or easier to access for women in the northern and southern regions. The project is a collaborative partnership with US- and Montenegro based scholars, the International Olympic Committee, the Montenegrin Olympic Committee, the United Nations Development Program, the Office for Gender Equality of the Ministry for Human and Minority Right of Montenegro, and NGO Inovativnost. Reflections and insights on multi-institutional partnerships in the study gender and sport participation will be offered.

  14. Iseloomuga majad, vol 1 / Ene Läkk

    Index Scriptorium Estoniae

    Läkk, Ene, 1957-

    2008-01-01

    Eesti Arhitektide Liidu korraldatud konkursi "Eramu 2006-2007" nominendid: 1. Funkvilla Nõmmel (arhitekt Andres Ringo); 2. Villa Tabasalus (arhitekt Tiit Trummal); 3. Sõlmes alumiiniummaja (arhitektid Peeter Pere, Urmas Muru); 4. Etnomodernne palkmaja (arhitektid Tauno Aadma, Laur Pihel); 5. Villa Kurepesa Pirita jõekäärus (arhitekt Kalle Rõõmus); 6. Vaskvilla Võrus (arhitekt Karmo Tõra)

  15. Iseloomuga majad, vol 2 / Ene Läkk

    Index Scriptorium Estoniae

    Läkk, Ene, 1957-

    2008-01-01

    Eesti Arhitektide Liidu korraldatud konkursi "Eramu 2006-2007" nominendid: 1. Vonklev arhitektimaja (arhitekt Karmo Tõra); 2. Betoonmaja Kloogarannas (arhitektid Laur Pihel, Tauno Aadma); 3. Funktalu Jõgisool (arhitekt Jaak Huimerind); 4. Villa Kakumäel (arhitekt Tiina Kaljundi); 5. Räästamaja Suurupis (arhitekt Raul Vaiksoo); 6. Klotsidest maja (arhitekt Eero Palm, kaasautor Kristiina Ott)

  16. Tööjuhendid loovuse arendajana / Ene Lind

    Index Scriptorium Estoniae

    Lind, Ene

    2001-01-01

    Loovuse arendamine tööõpetuse ja käsitöö tunnis. Katsest Tallinna koolide 2. ja 4. klasside õpilaste seas, milles katsetati kaht töö raskusastme ning kasutatavate materjalide ja töövahendite poolest erinevat tööjuhendit. Katse järel viidi läbi küsitlus, kus lapsed andsid hinnangu ja kommenteerisid tööjuhendi järgi iseseisvalt töötamist tööõpetuse tunnis

  17. Videomuinasjutt algastme eesti keele tundi : miks ka mitte? / Ene Peterson

    Index Scriptorium Estoniae

    Peterson, Ene

    2002-01-01

    Rets. : Tungal, Leelo. Videomuinasjutt "Printsess Mari ja konn Konrad" [Videosalvestis] = A fairytale on video "Princess Mari and frog Konrad". - Tallinn : Elav Teadus, 1993. - 1 videokassett (VHS) (120 min) + 1 raamat (32 lk.)

  18. 500-tonniste muldseintega elamu / Ene Läkk, Helen Urbanik

    Index Scriptorium Estoniae

    Läkk, Ene, 1957-

    2000-01-01

    Arhitekt Rick Joy projekteeritud arst John Palmeri villa USAs Arizona osariigis, Santa Catalina mäestiku jalamil. Hoone ühes osas asuvad perekonna eluruumid, teine on avatud ruum. Pressitud muldseinad ja liblikatiiva kujulised terasest katusesiilud jäljendavad ümbritseva maastiku vorme. Suuri seina- ja aknapindu katab raamimata klaas.

  19. Põllumajandussaaduste turg : [1994-2003 Eestis] / Ene Maadvere

    Index Scriptorium Estoniae

    Maadvere, Ene, 1948-

    2004-01-01

    Ilmunud ka: Agriculture and rural development : overview 2003/2004. - Tallinn, 2004, lk. 124-132. Põllumajandussaaduste ekspordimaht vähenes 2003. a. eelmise aasta sama perioodiga võrreldes 0,4%, importmaht aga suurenes 7%. Tabelid. Diagrammid. Graafikud

  20. Phytochemical Screening and Characterization of Olean-18-ene ...

    African Journals Online (AJOL)

    http://dx.doi.org/10.4314/star.v4i1.15 · AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More about AJOL · AJOL's Partners · Terms and Conditions of Use · Contact AJOL · News. OTHER RESOURCES... for Researchers · for Journals · for Authors · for Policy Makers ...

  1. Sotsiaal-majanduslik staatus tervise tausttegurina / Margo Kikas, Ene Lausvee

    Index Scriptorium Estoniae

    Kikas, Margo

    2008-01-01

    Uuringust seoste kohta Eesti inimeste tunnetatud tervise ning sotsiaal-majandusliku ja psühhosotsiaalse seisundi vahel. Uuring tugineb Tallinna Ülikooli sotsiaaltöö eriala üliõpilaste poolt aastatel 2001-2002 tehtud anonüümsel ankeetküsitlusel põhiliselt toimetulekuraskuste, krooniliste haiguste, puudega või riskikäitumisega isikute seas

  2. Prantsusmaale oma lõhna looma / Ene Poll

    Index Scriptorium Estoniae

    Poll, Ene

    2001-01-01

    Prantsusmaa väikelinnas Grasses, maailma parfümeeriakeskuses, on võimalik tutvuda Galimardi vabriku tootmisprotsessiga, lõhnaõli muuseumiga ja lõhnaloomisstuudios valmistada endale ise ainukordne parfüüm

  3. Otsused andmekandjate kohta kriminaalmenetluses: proportsionaalsusnõudega arvestamine / Eneli Laurits

    Index Scriptorium Estoniae

    Laurits, Eneli

    2016-01-01

    Riigikohtu lahendist nr. 3-1-1-57-12, proportsionaalsuse nõude rakendamisest asitõendi (andmekandja) täieliku hävitamise korral alamaastmete kohtute praktikas. Sisaldab 2013-2015 aastate asjakohast kohtupraktikat

  4. Preparation of Novel Hydrolyzing Urethane Modified Thiol-Ene Networks

    Science.gov (United States)

    2011-10-25

    networks will influence the rate in which water is absorbed into the film. This work represents the preliminary characterization of the hydrolysis of...Linossier, I.; Peron , J.J.; Langlois, V.; Vallee-Rehel, K. Antifouling activity of marine paints: Study of erosion. Prog. Org. Coat. 2007, 60, 194-206

  5. Hüdra aastasada / Ene-Reet Soovik

    Index Scriptorium Estoniae

    Soovik, Ene-Reet

    1997-01-01

    Arvustus: Galsworthy, John. Peatüki lõpp : Üle jõe / tlk. Jüri ja Liisi Ojamaa. Tallinn : Eesti Raamat, 1997; Amis, Kingsley. qnneseen Jim / tlk. Urve Hanko. Tallinn : Eesti Raadio, 1997; Boyd, William. Sinine pärastlõuna / tlk. Virve Krimm. Tallinn : Varrak, 1997. Briti XX saj. kirjanduse ülevaade

  6. Modernse arhitektuuri ja okupatsioonimuuseumi liit / Ene Läkk

    Index Scriptorium Estoniae

    Läkk, Ene, 1957-

    2001-01-01

    Okupatsioonimuuseumi ideest ja teostamisest. Avaliku arhitektuurivõistluse "Lähimineviku okupatsioonide muuseum" võitjad: I preemia: Indrek Peil, Siiri Vallner, II preemia Tiina Teng, Peeter Loo, III preemia Reio Avaste (AS Arhitektuuribüroo Eek & Mutso), ostupreemia: Margus (?) Kaasik, Kalle Komissarov, Andres Ojari, Indrek Tiigi, Ilmar Valdur (Arhitektuuribüroo Kolm Pluss Üks OÜ), ostupreemia: Andres Kask

  7. Ehte näitamise kunst / Ene Poll

    Index Scriptorium Estoniae

    Poll, Ene

    2009-01-01

    Tanel Veenre isikunäitus "Ehe" Rotermanni Nisurukkiveskis 24.-30. oktoobrini. Eksponeeritud 29 ehet on valminud viimase kolme aasta jooksul. Näitusega koos ilmus autoriraamat "Ehe", mis räägib kunsti ja kunstniku suhtest

  8. Fluoriidid Eesti elanike joogivees / Astrid Saava, Ene Indermitte

    Index Scriptorium Estoniae

    Saava, Astrid, 1938-

    2007-01-01

    Eesti elanike joogivee-uuringust, mis tehti 2004. aastal Eesti Stomatoloogia Seltsi tellimisel. Lisad: Eesti elanike joogivee fluoriidisisalduse kaart. Elanike jaotus tarbitava joogivee fluoriidisisalduse alusel maakonniti

  9. Kooli tehnoloogiaõpetus tuleb atraktiivseks muuta / Ene Lind

    Index Scriptorium Estoniae

    Lind, Ene, 1954-

    2010-01-01

    Üleeuroopalisest tehnoloogiaõppe edendamise projektist "Understanding and Providing a Developmental Approach to Technology Education" - UPDATE ("Tehnoloogiaõpetuse arenguvõimaluste mõistmine ning rakendamine"). Jyväskylä Ülikooli juhtimisel tegid koostööd 16 Euroopa haridus- ja teadusasutust, Eestit esindas Tallinna Ülikooli tööõpetuse osakond koos Eesti Tehnoloogiakasvatuse Liiduga

  10. Minimalismi asemele hiilib barokk / Ene Läkk

    Index Scriptorium Estoniae

    Läkk, Ene, 1957-

    1999-01-01

    Trendikatest jõulukompositsioonidest. Nõu annavad Stockholmi kuusekaunistamise maailmameistrivõistluse maailmameistrid Taivo Piller ja Mart Haber. T. Pilleri parim õpetaja - norra lilleseadja Linda Johansen.

  11. Ainenõukogu tähelepanekuid / Ene Laanes

    Index Scriptorium Estoniae

    Laanes, Ene

    2003-01-01

    Üleriigiline kehalise kasvatuse ainenõukogu peab vajalikuks juhtida haridusametnike, koolijuhtide ja kooli omanike tähelepanu kehalise kasvatuse ainekava läbiviimisel üles kerkinud probleemidele: meditsiinilisse erigruppi kuuluvate õpilaste hindamine kehalise kasvatuse aines, kehalise kasvatuse tunniks vajalikud harjutuspaigad ja vahendid, nädalatundide arv

  12. Production of 4-Ene-3-ketosteroids in Corynebacterium glutamicum

    Directory of Open Access Journals (Sweden)

    Julia García-Fernández

    2017-10-01

    Full Text Available Corynebacterium glutamicum has been widely used for the industrial production of amino acids and many value-added chemicals; however, it has not been exploited for the production of steroids. Using C. glutamicum as a cellular biocatalyst we have expressed the 3β-hydroxysteroid dehydrogenase/isomerase MSMEG_5228 from Mycobacterium smegmatis to demonstrate that the resulting recombinant strain is able to oxidize in vivo C19 and C21 3-OH-steroids to their corresponding keto-derivatives. This new approach constitutes a proof of concept of a biotechnological process for producing value-added intermediates such as 4-pregnen-16α,17α-epoxy-16β-methyl-3,20-dione.

  13. Võrumaa mitmekesisuse võlu / Ere Raag ; intervjueerinud Ene Riet

    Index Scriptorium Estoniae

    Raag, Ere

    2015-01-01

    Võrumaa Keskraamatukogu pearaamatukoguhoidja räägib, kuidas ja miks valis ta oma erialaks raamatukogunduse, kus ja kellena ta on töötanud ning mida on raamatukogundus talle andnud. Arutletakse ka raamatukogunduse ning raamatukoguhoidja tuleviku üle

  14. 3α-Dimethylamino-20-(N-methylacetamidopregn-5-ene

    Directory of Open Access Journals (Sweden)

    S. Yousuf

    2011-11-01

    Full Text Available The title compond, C26H44N2O, is an steroidal alkaloid isolated from the medicinally important plant Sarcococca saligna. The molecule consists of four fused rings (A–D, having chair, half-chair, chair and envelope conformations, respectively. The dimethylamino group is axially oriented on ring A, whereas the (N-methylacetamidoethyl group is attached equatorially on ring D. The crystal structure is stabilized only by van der Waals forces.

  15. Den eneste ene - hvordan etniske minoritetsunge i Danmark danner par

    DEFF Research Database (Denmark)

    Singla, Rashmi

    Rapporten handler om etniske unges måde at danne par på. Rapporten er baseret på en større empirisk undersøgelse gennemført af forfatteren i 2003 og er desuden inspireret af forfatterens egen ph.d.-afhandling: "Ungdom, etnicitet og psychosocial intervention" (2000, Københavns Universitet...

  16. Tuul väljal / Ene-Reet Soovik

    Index Scriptorium Estoniae

    Soovik, Ene-Reet

    1997-01-01

    Arvustus: Woolf, Virginia. Esseed/ inglise k. tlk. Malle Talvet, Jaak Rähesoo. Tallinn : Hortus Litterarum, 1997; Woolf, Virginia. Orlando : elulugu / inglise k. tlk. Riina Jesmin. Tallinn : Perioodika, 1997. (Loomingu Raamatukogu; 31-34)

  17. Põllumajandussaaduste eksport ja import Eestis 1995. aastal / Ene Maadvere

    Index Scriptorium Estoniae

    Maadvere, Ene, 1948-

    1996-01-01

    Tabelid: Kaupade eksport ja import Eestis; Põhiliste kaubagruppide osatähtsuse muutumine toidukaupade ekspordis; Hinnad; Mõnede toidukaupade eksport 1995.a. võrreldes 1994.a.; Imporditud toidukaupade struktuur võrreldes 1995.a.; Nisuhind Chicago börsil. Diagrammid: Liha-piima tootmine, tarbimine, eksport ja tonnides; Põllumajandussaaduste eksport 1995.a. riikide lõikes

  18. Euroopa kõige ilusamad hotellid / Ene Läkk

    Index Scriptorium Estoniae

    Läkk, Ene, 1957-

    2007-01-01

    Hotel Puerta America Madridis, mille on kujundanud 19 maailmakuulsat disainerit. Hotell Luzernis, kus laekujunduses on kasutatud filmikaadreid. Hotell Lute Suites Hollandis. Duomo Hotel Riminis, kujundus Ron Arad'ilt. Kruisherenhotel Maastrichtis

  19. Tandem Wittig-ene reaction approach to kainic acid

    Digital Repository Service at National Institute of Oceanography (India)

    Majik, M.S.; Parameswaran, P.S.; Tilve, S.G.

    bromide (1 equiv) in CH 3 CN (2 mL) over a period of 10 min in an ice cold water bath. The reaction mixture was stirred for1hatroom temperature and then concentrated under reduced pressure. Purification of residue by column chromatography on silica gel... solution of bromoacetyl bromide (1.2 equiv) in CHCl 3 (1 mL) at 0 °C over a period of 10 min. The reaction mixture was then stirred for1hatroom temperature and diluted with water (20 mL), and product was extracted with CHCl 3 (3 × 20 mL). The organic phase...

  20. Mentor - mis see veel on? / Ene-Silvia Sarv

    Index Scriptorium Estoniae

    Sarv, Ene-Silvia

    2001-01-01

    Mentori rollist praktikantide, kutseaastat sooritavate ja uute õpetajate juhendajana, klassijuhatajate ja aineõpetajate nõustajana, kontaktisikuna noorte õpetajate ettevalmistamisel ülikoolis. TPÜ magistrantide mentorirühma projektseminaril "Mentori roll lähituleviku Eesti koolis" ja TÜ mentorirühma õppustel olid teoreetilise põhikursuse kõrval arutusel mentorite praktilise rakendamise võimalused. Analüüsi põhjal tuletati mentorluse neli võimalikku stsenaariumi

  1. Lapse ülalpidamise hind / Ene-Margit Tiit

    Index Scriptorium Estoniae

    Tiit, Ene-Margit, 1934-

    2005-01-01

    Tutvustav artikkel sotsiaalministeeriumi tellimusel läbiviidud uuringust, mille eesmärgiks oli välja töötada lapse kõikidest vajadustest lähtuv ühtne metoodika lapse ülalpidamiskulude arvutamiseks ühes kuus. Tabelid. Diagrammid. Vt. ka lk. 15-16: Kaplan, Signe. Metoodika kasutamisvõimalusi

  2. Õppimine - argine? / Ene-Silvia Sarv

    Index Scriptorium Estoniae

    Sarv, Ene-Silvia

    2001-01-01

    Tagasivaateliselt õppimise uurimisest ja õppimise tähtsuse suurenemisest 20. sajandil. Õppimise käsitlusi. Aju ja õppimine, ajupõhise õppimise põhimõtted ja seosed õppekava, õpetamise ja hindamisega

  3. People in Ida-Virumaa : between two cultures / Ene Must

    Index Scriptorium Estoniae

    Must, Ene

    2002-01-01

    Peamistest probleemidest Ida-Virumaal, Sillamäel ja Narvas. Kohalike elanike seas läbi viidud Eesti sotsioloogide, Eesti integratsioonifondi ja Saar Polli uuringufirma uurimusest, kus uuriti nende sidemeid naaberriikidega, majanduslikku olukorda ning nende võimalikku elukoha muutust seoses majandusliku olukorraga Eestis ja liitumisega EL-iga. Kokkuvõtted uuringu tulemustest. Diagrammid

  4. Ühest poisist : film ja raamat / Ene Vainik

    Index Scriptorium Estoniae

    Vainik, Ene

    2002-01-01

    Mängufilm "Suur poiss ja väike poiss" ("About a Boy") : režissöörid Paul ja Chris Weitz : Suurbritannia - Ameerika Ühendriigid - Prantsusmaa 2002. Võrdlus filmi ja selle aluseks olnud Nick Hornby romaani "Ühest poisist" vahel

  5. Raesaalis lauldakse armastuse ajast / Ülle Jantson

    Index Scriptorium Estoniae

    Jantson, Ülle

    2008-01-01

    Lumepromenaadi sarja teine kontsert "See A-tähega sõna" 7. dets. 2008 Pärnu Raekojas, esinevad Pärnu noored lauljad ja instrumentalistid Toomas Volli juhendamisel, esitatakse jõulumuusikat, noorte omaloomingut, eesti heliloojate laule

  6. Kunstiõppurid tulid üle jõe

    Index Scriptorium Estoniae

    2001-01-01

    18. jaan. avati Pärnu lasteraamatukogus Agape kunstiklassi klaasimaalide näitus. Pärnu haigla traumaosakonnas on Agape täiskasvanute kunstistuudios valminud klaasimaalide ja Leili Volli graafika näitus

  7. Hõbehääled kullale / Ülle Hallik

    Index Scriptorium Estoniae

    Hallik, Ülle, 1963-

    2003-01-01

    Toomas Volli koor "Argentum Vox" naasis Praha rahvusvaheliselt 13. jõulu- ja advendilaulude koorikonkurss-festivalilt (28.-30. nov.) grand prix', kuldmedali ja eripreemiaga kõrge vokaalkultuuri eest

  8. Medieval and Early Modern Suburban Site in Tallinn, Tartu Road 1: Contexts and Structures / Ulla Kadakas, Guido Toos, Anneli Kalm ... [jt.

    Index Scriptorium Estoniae

    2013-01-01

    Väljakaevamised toimusid kvartalis, mille lõunaservas kulges ajalooline Tartu maantee, ning paarisaja meetri kaugusel asus ülepääs Härjapea jõest - nn Kivisild. Kinnistute põhistruktuur näib üsna püsivana, tegemist on pikkade pisut edela-kirde suunaliste kruntidega

  9. Töökogemus võib hakata pabereid asendama / Sirje Pärismaa ; kommenteerinud Ott Ojaveer, Merike Kaste, Mart Kalm, ... [jt.

    Index Scriptorium Estoniae

    Pärismaa, Sirje, 1958-

    2016-01-01

    VÕTA (varasema õpi- ja töökogemuse arvestamine) laiendamise kavast. Kui kooskõlastusringil olev haridusseaduse eelnõu vastu võetakse, võib põhikoolitunnistuseta kandideerida gümnaasiumi, bakalaureusediplomita magistrantuuri ja ehk gümnaasiumi­tunnistuseta ülikooligi

  10. Sõdade-vaheline Pärnu kuurort = Pärnun kylpylätoiminta sotien välisenä aikana / Mart Kalm

    Index Scriptorium Estoniae

    Kalm, Mart, 1961-

    2004-01-01

    Pärnu kuurordi taastamisest. Mudaravila arhitektuurikonkurssidest. 1927. a. avati O. Siinmaa, E. von Wolffeldti ja A. Nürnbergi projekti järgi valminud supelasutus. Hoone sisemise dekoori ja mööbli kavandas O. Siinmaa, skulpturaalsed osad teostas Voldemar Mellik. 1925. a. sai Pärnu linnaarhitektiks O. Siinmaa. 1937. a. valmis Rannahotell (arhitektid O. Siinmaa, A. Soans), 1939. a. Rannakohvik (arhitekt O. Siinmaa) jm. Bibliograafia joonealustes märkustes

  11. Muusika : Muusika igaks asendiks ja meeleoluks. Plaadid kauplusest Lasering

    Index Scriptorium Estoniae

    2005-01-01

    Heliplaatidest: Robbie Williams "Greatest Hits", Gotan Project "Insipracion Espiracion", Manic Street Preachers "Lifeblood", Daddy G "DJ Kicks", Eminem "Encore", Kalm "Generalissimus Kalm", U2 "How to dismantle an atomic bomb"

  12. Structure-activity relationships of 3-deoxy androgens as aromatase inhibitors. Synthesis and biochemical studies of 4-substituted 4-ene and 5-ene steroids.

    Science.gov (United States)

    Nagaoka, Masao; Watari, Yoko; Yajima, Hiromi; Tsukioka, Kaoru; Muroi, Yasuyo; Yamada, Keiko; Numazawa, Mitsuteru

    2003-08-01

    As part of our investigation into the structure-activity relationship of a novel class of aromatase inhibitors, two series of 3-deoxy androgens, androst-5-en-17-ones with a non-polar alkoxy (5 and 6), alkyl (20-22), or phenylalkyl (23 and 24) group at C-4beta and 4-acyloxyandrost-4-en-17-ones (29-32, and 34) were synthesized and evaluated. The 4beta-alkyl and 4beta-phenylalkyl compounds were obtained through reaction of 4alpha,5alpha-epoxy steroid (8) with RMgBr (R: alkyl and phenylalkyl) followed by dehydration of the 4beta-substituted 5alpha-hydroxy products (15-19) with SOCl(2) as key reactions. Acylation of 4alpha,5alpha-diol (25) with (RCO)(2)O in pyridine and subsequent dehydration with SOCl(2) gave the 4-acyloxy steroids. All of the steroids studied, except for 4-acetoxy-19-ol (34) that was a non-competitive inhibitor of human placental aromatase, blocked aromatase activity in a competitive manner. 4-Benzoyloxy- and 4-acetoxy steroids (31) and (32) were the most powerful inhibitors of aromatase (K(i)=70 and 60nM, respectively). Elongation of an acetoxy group in a series of 4-acyloxy steroids or a methyl group in a series of 4beta-alkyl steroids decreased affinity for aromatase principally in relation to carbon number of the acyl or alkyl function. The present findings are potentially useful for understanding the spatial and electronic nature of the binding site of aromatase as well as for developing effective aromatase inhibitors.

  13. Vene piirist ja piirikaitsest Eesti kagunurgas keskajal / Heiki Valk

    Index Scriptorium Estoniae

    Valk, Heiki, 1959-

    2006-01-01

    Piiri olemasolu mõjust piirialade elanike elukorraldusele ja sotsiaalsele staatusele, piiriala kontrollimiseks vajalikust infrastruktuurist, piirikaitsekorraldusest ning Siksälä kalme relvahaudadest

  14. Lõuna 2a villa restaureerimine Pärnus = Restoration of Lõuna 2a villa in Pärnu / Jaak Huimerind, Mari Kurismaa, Mart Kalm ; kommenteerinud Liina Jänes

    Index Scriptorium Estoniae

    Huimerind, Jaak, 1957-

    2015-01-01

    Villa Pärnus Lõuna tänav 2a, valminud 1936, restaureeritud 2014. Hoone arhitektid Olev Siinmaa, Anton Soans, restaureerimisarhitekt Jaak Huimerind, sisearhitekt Mari Kurismaa. Eesti Kultuurkapitali Arhitektuuri sihtkapitali restaureerimispreemia 2014

  15. Prügilill, vana hõng ja minimalism / Ene Läkk

    Index Scriptorium Estoniae

    Läkk, Ene, 1957-

    2000-01-01

    Kahest äärmusest praeguses aiakujunduses: minimalismist ja vanahõngulisest, hästi organiseeritud segadusest. Erinevatest materjalidest terrassist, "woodland'ist", moesolevatest prügitaimedest. Trendinäiteks Saksa saatkonna haljasalast Tallinnas (arhitekt R. Herms, teostus Ü. ja U. Grishakov). Soovitusi maastikuarhitekt Ü. Grishakovilt

  16. Vaesus ja ebavõrdsus = Poverty and inequality / Mari Kreitzberg, Ene-Margit Tiit

    Index Scriptorium Estoniae

    Kreitzberg, Mari

    2004-01-01

    Vaesuse ja ühiskonna sotsiaalsest sidususest, vaesuse määratlemisest, subjektiivsest vaesusest, sissetuleku- ja kuluvaesusest, vaesuspiirist, absoluutsest ning suhtelisest vaesusest. Diagrammid. Tabelid. Graafikud

  17. Whats 'App-ening': the help of new technologies in nutrition in digestive diseases.

    Science.gov (United States)

    Ankersen, Dorit V; Weimers, Petra; Burisch, Johan

    2017-09-01

    The aim of this study is to review the basic concepts of electronic health (eHealth), with a focus on its nutritional applications and its usefulness for digestive diseases. eHealth applications for the treatment and monitoring of digestive disease are growing in number. ehealth helps patients in coping with their disease by promoting self-management, which increases adherence to medical treatment and diets, and leads to an improved quality of life. For irritable bowel syndrome (IBS), there are multiple applications that provide dietary advice, for example, a low FODMAP (Fermentable Oligo, Mono, Disaccharides And Polyols) diet. However, many applications lack a symptom scoring function and do not include a module for assisting the essential reintroduction of high FODMAP foods. In general, there are very few applications that enable direct patient communication with healthcare professionals. A more holistic approach that educates patients and enables them to communicate directly with eCare provider through a web application is one of the functions most requested by patients. eHealth solutions for digestive diseases have a supportive function and a positive impact on patients. However, there is a need to increase patient education and further develop the possibility for care team-patient communication within eHealth solutions.

  18. Õpetaja haridus, kooliharidus ja ühiskond / Ene-Silvia Sarv

    Index Scriptorium Estoniae

    Sarv, Ene-Silvia, 1945-

    1999-01-01

    Rahvusvahelise Haridusentsüklopeedia tekstist ja Eesti õpetajakoolituse uuendamise kavadest inspireeritud mõtisklus. Teoreetilised teadmised, võimed, eeldused, mida peetakse õpetajale vajalikuks, on seotud haridusele omastatavate eesmärkidega

  19. N,N?-(Ethane-1,2-diyldi-o-phenyl?ene)bis?(pyridine-2-carboxamide)

    OpenAIRE

    Sarkar, Shuranjan; Lee, Hong-In

    2011-01-01

    The title molecule, C26H22N4O2, is centrosymmetric and adopts an anti conformation. Two intramolecular hydrogen bonds, viz. amide–pyridine N—H...N and phenyl–amide C—H...O, stabilize the trans conformation of the (pyridine-2-carboxamido)phenyl group about the amide plane. In the crystal, the presence of weak intermolecular C—H...O hydrogen bonds results in the formation of a three-dimensional network.

  20. Meistrid : [luuletused] / Lajos Kassák ; tlk. Ene Asu-Õunas

    Index Scriptorium Estoniae

    Kassák, Lajos

    2004-01-01

    Sisu: Meistrid ; "Tulin kõrvaltänavast 11 sõbraga, olime maakad ja virginia ..." ; "Siniste linade all lamavad minu surnud ..." ; "Seisan värava juures, unustatud maja värava juures ehavalguses ..." ; Kauge tervitus ; Kõnelen meie surnutest ; Täiskuu ; Emake valvab meid ; Suletud värava ees ; Béla Bartók ; Surnud sõdurid ; Ühtsus ; Tinane vihm ; Öö segadus ; Meister ja õpilane ; Sealpool piiri ; Hõbehall ; Arusaamatu pilt ; Reekviem ühele naisele. Andmeid autori kohta lk. 7-8

  1. Animafilm mõistmisest ja tolerantsist jõuab Kuressaarde / Ene Kallas

    Index Scriptorium Estoniae

    Kallas, Ene

    2008-01-01

    Kuressaarde jõuab koos ajaloolase David Vseviovi sissejuhatava loenguga animadraama "Persepolis" : režissöörid Marjane Satrapi (oma koomiksi järgi) ja Vincent Paronnaud : Prantsusmaa - Ameerika Ühendriigid - Iraan 2007

  2. Thiol-ene crosslinking polyamidoamine dendrimer-hyaluronic acid hydrogel system for biomedical applications.

    Science.gov (United States)

    Bi, Xiangdong; Liang, Aiye; Tan, Yu; Maturavongsadit, Panita; Higginbothem, Ashley; Gado, Togor; Gramling, Abigail; Bahn, Hanna; Wang, Qian

    2016-01-01

    A series of alkene functionalized polyamidoamine (PAMAM) dendrimers were synthesized to prepare in situ forming hydrogels with varied gelation time and mechanical properties through crosslinking with thiolated hyaluronic acid (HS-HA). By varying the alkenyl groups on the dendrimers, the gelation time displayed a large range from 8 seconds to 18 hours, and the modulus of the hydrogels ranged from 36 to 183 Pa under experimental conditions. Investigation by (1)H-NMR spectroscopy revealed that the gelation time and the stiffness of the hydrogels were governed by the degree of electron deficiency of alkenyl groups on the dendrimers. This research provided a systematic study on the relationship between chemical structures versus gelation time and mechanical properties of hydrogels, which could guide the way to synthesize in situ forming hydrogels with designated gelation time and stiffness for biomedical applications. Further, a RGD peptide was attached to the PAMAM dendrimers to enhance cell attachment and proliferation. Viability assays of Human Umbilical Vein Endothelial Cells (HUVEC) in the synthesized hydrogels demonstrated the biocompatibility of the hydrogels after 48 hours of culturing, and the RGD peptide improved the viability of HUVEC cells in hydrogels. We believe the PAMAM/HA hydrogel system is a tuneable and biocompatible system for diverse biomedical applications.

  3. How newness enters a country : reception of the "postcolonial" in Estonia / Ene-Reet Soovik

    Index Scriptorium Estoniae

    Soovik, Ene-Reet, 1968-

    2006-01-01

    Postkolonialistliku tõlkekirjanduse retseptsiooni Eestis vaadeldakse kolme romaani põhjal: Arundhati Roy "Väikeste Asjade Jumal" (eesti k. 1997 ja 2004), Salman Rushdie "Mauri viimane ohe" (eesti k. 2001 ja 2008), Ben Okri "Näljutatud tee" (eesti k. 1998)

  4. Terves kehas terve vaim - a sound mind in a sound body / Ene Kelder

    Index Scriptorium Estoniae

    Kelder, Ene

    2012-01-01

    Pühajärve Haridusseltsi täiskasvanutele suunatud projektist, mille eesmärgiks oli suurendada täiskasvanud õppijate hulka piirkonnas ning praktiseerida aktiivset eluhoiakut ja tervislikke eluviise

  5. Muudatused sisemajanduse koguprodukti arvestustes = Changes in the methodology of macroeconomic statistics / Ene Kaldma

    Index Scriptorium Estoniae

    Kaldma, Ene

    2004-01-01

    2004. aastal tehtud muudatused makromajandusstatistika metoodikas tõid kaasa 1993.-2003. aasta sisemajanduse koguprodukti (SKP) ja selle komponentide suuruse korrigeerimise. Tabel: Sisemajanduse koguprodukt turuhindades kvartalite kaupa enne ja pärast metoodika muutumist, 1993-2003

  6. Novel approaches for using dehydrogenases and ene-reductases for organic synthesis

    NARCIS (Netherlands)

    Gargiulo, S.

    2015-01-01

    Oxidation of alcohols is a reaction of major interest for organic chemistry. However, the most common chemical routes developed so far involve the use of toxic or hazardous reagents or catalysts that often lack good chemoselectivity. In this respect, alcohol dehydrogenases (ADHs) represent a very

  7. Raport : Eesti eramu 2006 - 2007 / Ene Läkk ; interv. Urmas Oja

    Index Scriptorium Estoniae

    Läkk, Ene, 1957-

    2008-01-01

    Eesti Arhitektide Liidu korraldatud konkursist parima eramu tiitlile, žürii koosseis. Väljavalitud töödest koostatakse Arhitektuuri- ja Disainigaleriis näitus "Eramu 2006-2007". Viimaste aastate eramuarhitektuurist, muudatustest ruumiplaneeringus, materjalivalikust

  8. Emotsioonisõnad töises keskkonnas - millest kõnelevad loetelukatsete tulemused? / Ene Vainik

    Index Scriptorium Estoniae

    Vainik, Ene

    2007-01-01

    Emotsioonisõnade esiletulekut tööl ja mujal võrreldakse 2001. aastal läbi viidud loetelukatsetes, tulemuste semantilise analüüsi põhjal tehakse järeldused koha mõju üle emotsioonisõnavarale

  9. 1-(3,5-Dichlorophenyl-3-(2-methoxyphenyltriaz-1-ene

    Directory of Open Access Journals (Sweden)

    Behrouz Notash

    2012-03-01

    Full Text Available The title molecule, C13H11Cl2N3O, is almost planar and adopts a trans conformation with respect to the –N=N– bond; the dihedral angle between the rings is 3.47 (2°. The N—N bond lengths indicate the presence of single- and double-bond characters and hence the –N=N—NH– moiety. In the crystal, inversion dimers linked by pairs of N—H...Cl hydrogen bonds occur, and C—H...π and π–π stacking interactions are also observed.

  10. Learning Organizations. Studiu exploratoriu asupra Consiliilor Judeţene din România

    OpenAIRE

    Diana GHIURA

    2012-01-01

    This article is the result of an ample study centered on the concept of learning organizations, more precisely on the degree in which Romanian County Councils are learning organizations. In order to analyze this, a research instrument has been sent to every County Council. This tool has been developed by three Harvard professors: David A. Garvin, Amy C. Edmondson and Francesca Gino who also published, in 2008, an article in Harvard Business Review ‘Is Yours A Learning Organization?’. Their ar...

  11. Kelle emotsioonile anda teksti ette lugedes hääl? Vaatlus mitmest perspektiivist / Ene Vainik

    Index Scriptorium Estoniae

    Vainik, Ene, 1964-

    2014-01-01

    Püütakse esile tuua, milliste inimloomusest tulenevate tõsiasjadega peab arvestama, kui hakata sünteeskõnele emotsioone matkivat prosoodiat lisama. Koondatud on lähenemised erinevatest teadusharudest

  12. Comparison of photopolymerizable thiol-ene PEG and acrylate-based PEG hydrogels for cartilage development.

    Science.gov (United States)

    Roberts, Justine J; Bryant, Stephanie J

    2013-12-01

    When designing hydrogels for tissue regeneration, differences in polymerization mechanism and network structure have the potential to impact cellular behavior. Poly(ethylene glycol) hydrogels were formed by free-radical photopolymerization of acrylates (chain-growth) or thiol-norbornenes (step-growth) to investigate the impact of hydrogel system (polymerization mechanism and network structure) on the development of engineered tissue. Bovine chondrocytes were encapsulated in hydrogels and cultured under free swelling or dynamic compressive loading. In the acrylate system immediately after encapsulation chondrocytes exhibited high levels of intracellular ROS concomitant with a reduction in hydrogel compressive modulus and higher variability in cell deformation upon compressive strain; findings that were not observed in the thiol-norbornene system. Long-term the quantity of sulfated glycosaminoglycans and total collagen was greater in the acrylate system, but the quality resembled that of hypertrophic cartilage with positive staining for aggrecan, collagens I, II, and X and collagen catabolism. The thiol-norbornene system led to hyaline-like cartilage production especially under mechanical loading with positive staining for aggrecan and collagen II and minimal staining for collagens I and X and collagen catabolism. Findings from this study confirm that the polymerization mechanism and network structure have long-term effects on the quality of engineered cartilage, especially under mechanical loading. Copyright © 2013 Elsevier Ltd. All rights reserved.

  13. Interview; kunst of kliederen; “De ene graffiti is de andere niet”

    NARCIS (Netherlands)

    Keus, R; Vanderveen, G.N.G.

    2010-01-01

    De aanpak van graffiti staat bij veel gemeenten hoog op de agenda. Het tegengaan van overlast en verloedering is hierbij meestal de belangrijkste reden. Maar om een aanpak echt te laten slagen, moet er volgens onderzoekster Gabry Vanderveen eerst goed worden nagegaan welke vormen van graffiti voor

  14. Kinnisvarakorilus Süda tänaval / Ene Läkk

    Index Scriptorium Estoniae

    Läkk, Ene, 1957-

    2003-01-01

    Kriitiline vaatenurk tunneli rajamise plaanile läbi miljööväärtusliku Tallinna Süda tänava kvartali. Arhitektuuribüroode R-Konsultant ja Koot & Koot pakutavate erinevate lahenduste võrdlus. Arhitektide kommentaare

  15. Punane moon või kaine mõistus / Ene Läkk

    Index Scriptorium Estoniae

    Läkk, Ene, 1957-

    2001-01-01

    Nukuteatri uue hoone sisekujunduse võistluse I ja II koha töödest. I koha töö autorid Raivo Kotov ja Andrus Kõresaar, II koha töö autorid Ülar Mark, Liina Link, stilist Ain Nurmela ja arvutigraafik Tanel Viks büroost Mark & Tamm Arhitektid

  16. Tööjuhendid algklassi tööõpetuse tundides / Ene Lind

    Index Scriptorium Estoniae

    Lind, Ene

    2001-01-01

    Tekstiga tööst tööõpetuse tunnis, kirjalikust ja suulisest juhendamisest. Tallinna Pedagoogikaülikooli üliõpilaste poolt läbi viidud küsitlusest, milles osalesid 22 klassiõpetajat Tallinnast ja 15 mujalt vabariigist. Kõik pedagoogid andsid oma klassis ka tööõpetuse või käsitöö tunde. Küsitlusega selgitati, milline on tööjuhendite abil korraldatava iseseisva individuaalse töö osatähtsus noorema kooliastme tööõpetuse ja eri õppeainete tundides

  17. Tre tilfaelde af visceral leishmaniasis: det ene hos en HIV-positiv mand

    DEFF Research Database (Denmark)

    Balslev, U; Jonsbo, F; Junge, Jette

    1991-01-01

    Three cases of visceral leishmaniasis (kala-azar) are presented. One of these was in a 43-year-old patient with AIDS who was infected in Southern Spain. Another was in a man aged 25 years infected in West Africa. These cases are the first two adults to be reported in Denmark. The third case was a...

  18. Lean versus Quick Response Manufacturing og andre koncepter - er Lean den eneste ene?

    DEFF Research Database (Denmark)

    Michelsen, Aage U

    2005-01-01

    principper fra andre koncepter? Artiklen er opdelt i fire hovedafsnit. I første afsnit skitseres baggrunden for Lean. I andet afsnit skitseres kort indholdet i Lean. I tredje afsnit skitseres tankegangen bag Quick Response Manufacturing - et begreb, der har mange ligheder med Lean, men som har fokus på......Begrebet Lean har gennem de senere år vundet en enorm udbredelse. Antallet af publikationer om Lean har været eksplosivt stigende, og mange virksomheder har gennemført eller gennemfører Lean-projekter. Er Lean et nyt koncept? Er Lean den eneste løsning? Kan Lean kombineres med tankegange og...... løsning af en anden produktionsopgave. I fjerde afsnit skitseres kort relationerne mellem Lean og to andre begreber, Six Sigma og TPM, der begge har samme mål som Lean, men forskellige udgangspunkter og forskellige indfaldsvinkler....

  19. Riigipeade kokad - Chefs des Chefs / Sirkka Ruottinen, Indrek Kivisalu ; interv. Ene Ojaveski

    Index Scriptorium Estoniae

    Ruottinen, Sirkka

    2006-01-01

    Soome presidendi Tarja Haloneni ja Eesti presidendi Arnold Rüütli peakokad räägivad oma karjäärist presidendi peakoka ametini, kirjeldavad tavalist tööpäeva ja töökeskkonda presidendi köögis, iseloomustavad Soome ja Eesti toidukultuuri ning meenutavad huvitavaid seiku oma ametiajast. Vt. samas: Sirkka Ruottinen vastab küsimustele, mis puudutavad Riigipeade Peakokkade Klubi (Club des Chefs des Chefs) tegevust

  20. Me suudame ajaga kaasas käia / interv. Ene Riet

    Index Scriptorium Estoniae

    2003-01-01

    Eesti raamatukoguhoidjate VIII kongressi (9.-10. juuni 2002) delegaatitelt küsiti arvamust Eesti raamatukogunduse kohta, vastasid: Kalli Kuhi, Sirje Okapuu, Toomas Liivamägi, Sirje Lauring-Vaska, Helve Sreiberg, Irina Kruusla, Ädu Neemre, Tiit Margna, Valentina Brovina, Asko Tamme, Meeli Veskus

  1. Identification of fungal ene-reductase activity by means of a functional screening.

    Science.gov (United States)

    Romagnolo, Alice; Spina, Federica; Brenna, Elisabetta; Crotti, Michele; Parmeggiani, Fabio; Varese, Giovanna Cristina

    2015-06-01

    Bioeconomy stresses the need of green processes promoting the development of new methods for biocatalyzed alkene reductions. A functional screening of 28 fungi belonging to Ascomycota, Basidiomycota, and Zygomycota isolated from different habitats was performed to analyze their capability to reduce C=C double bonds towards three substrates (cyclohexenone, α-methylnitrostyrene, and α-methylcinnamaldehyde) with different electron-withdrawing groups, i.e., ketone, nitro, and aldehyde, respectively. Almost all the fungi showed this reducing activity. Noteworthy Gliomastix masseei, Mucor circinelloides, and Mucor plumbeus resulted versatile and effective, being able to reduce all the model substrates quickly and with high yields. Copyright © 2015 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.

  2. Tre tilfaelde af visceral leishmaniasis: det ene hos en HIV-positiv mand

    DEFF Research Database (Denmark)

    Balslev, U; Jonsbo, F; Junge, Jette

    1991-01-01

    Three cases of visceral leishmaniasis (kala-azar) are presented. One of these was in a 43-year-old patient with AIDS who was infected in Southern Spain. Another was in a man aged 25 years infected in West Africa. These cases are the first two adults to be reported in Denmark. The third case...

  3. Lendab nagu kotkas üle maailmas kogutud tarkuse / Richard Aroksaar ; intervjueerinud Ene Riet

    Index Scriptorium Estoniae

    Aroksaar, Richard

    2014-01-01

    Intervjuu eestlase Richard Aroksaarega, kes õppinud psühholoogiat ja raamatukogundust, töötab USA Rahvusparkide Ühenduse raamatukogus (National Park Service Library, NPS) süsteemi raamatukoguhoidja ja administraatorina

  4. Tre tilfaelde af visceral leishmaniasis: det ene hos en HIV-positiv mand

    DEFF Research Database (Denmark)

    Balslev, U; Jonsbo, F; Junge, Jette

    1991-01-01

    Three cases of visceral leishmaniasis (kala-azar) are presented. One of these was in a 43-year-old patient with AIDS who was infected in Southern Spain. Another was in a man aged 25 years infected in West Africa. These cases are the first two adults to be reported in Denmark. The third case was a...... was an 18 month old previously healthy boy, infected in Southern Spain. The symptomtology, diagnosis and treatment of the disease are discussed and it is stressed that serological diagnostic tests have limited value in HIV positive patients.......Three cases of visceral leishmaniasis (kala-azar) are presented. One of these was in a 43-year-old patient with AIDS who was infected in Southern Spain. Another was in a man aged 25 years infected in West Africa. These cases are the first two adults to be reported in Denmark. The third case...

  5. Eesti keele sõnavara afektikalduvus ja mis sellega peale hakata / Ene Vainik

    Index Scriptorium Estoniae

    Vainik, Ene, 1964-

    2012-01-01

    Praktilisest vajadusest väljakasvanud probleemist muuta eestikeelne sünteeskõne loomulikumaks ja emotsionaalselt usutavamaks. Artiklis keskendutakse leksikaalsele tasandile, arutletakse selle üle, millised sõnaklassid on enim emotsioonidega seotud

  6. DRUŽINSKA MEDIACIJA, KO SO OGROŽENE OTROKOVE KORISTI

    OpenAIRE

    Mendaš, Dominika

    2014-01-01

    Družinska mediacija se je v zadnjih letih tudi v Sloveniji uveljavila kot učinkovit način alternativnega reševanja družinskopravnih sporov. Družinski konflikti pogosto vključujejo tudi otroke, katerim moramo ob nenadnih življenjskih spremembah posvetiti posebno pozornost. Vključevanje otrok v mediacijo se je v praksi izkazalo kot pozitiven način, kako usmeriti pozornost udeleženih strank na ključno temo razprave varovanje otrokovih koristi. Mednarodni in nacionalni pravni akti dajejo pravn...

  7. Maja nagu kuupidest küla / Ene Läkk, Helen Urbanik

    Index Scriptorium Estoniae

    Läkk, Ene, 1957-

    2000-01-01

    Ühepereelamu Maastrichti lähedal. Projekti autorid Ettore Sottsass, Johanna Grawunder, arhitekt Oliver Layseca. Keskse siseõue ümber eraldiseisvaid kuupe ühendavad koridorid. Iga elutegevuse jaoks on omaette tsoon. Uksed peaaegu puuduvad. Sisekujunduses domineerib massiivne ja mahukas mööbel.

  8. Austraalia ilusaim rannikutee sõjaohvrite mälestuseks / Eneli Ivask

    Index Scriptorium Estoniae

    Ivask, Eneli

    2006-01-01

    Vt. ka Delovõje Vedomosti : Vkuss Zhizni 13. sept., lk. 4-5. Austraalia lõunarannikul, mitte kaugel Melbourneist on maailma kauneimaks peetav 400 km pikkune hingematvalt lummavate ja erinäoliste vaadetega rannikutee - Great Ocean Road

  9. Tootedisaineri töö peenmehaanika ja olemise jaatus / Ene Läkk

    Index Scriptorium Estoniae

    Läkk, Ene, 1957-

    2011-01-01

    Tootjate ja riskikapitalistide huvist tootedisaineri teenuse vastu. Eesti disainibüroodest MaDis-Stuudio (Matti Õunapuu), Ten Twelve (asutaja: Jukka Halttunen), Keha3 (Margus Triibmann, Tarmo Luisk, Pavel Sidorenko, juht: Ville Jehe) ja Iseasi (Martin Pärn, Sven Sõrmus, Edina Dufala-Pärn, Pent Talvet, Kaspar Torn), pakutavatest teenustest ja töödest

  10. "Ostanovimsja na mig v tishine..." : [luuletused] / Ene Mihkelson ; lühibiograafia: T. L. [Toomas Liiv

    Index Scriptorium Estoniae

    Mihkelson, Ene, 1944-2017-

    1999-01-01

    Sisu: "Ostanovimsja na mig v tishine..." / tlk. V. Fadin ; Rekviem / tlk. Marju Lauristin ; "Dom eto tolko godõ..." ; "Dvoje bezumtsev mezh nimi treti..." ; "Eva v Tapa zhivjot / tlk. Boris Tuch ; "Ljagushka prõgnula skvoz lezvije kossõ..." ; "Vse stantsii peremeshtshalis..." ; "Dõb iz trub stekajet..." ; "Vremja neuklonno i surovo..." / tlk. M. Korol ; Pjat tshassov kogda snega net / tlk. Jelena Salkova. Orig.: Reekviem ; "Konn hüppas vikatist läbi..." ; "Kõik jaamad segunesid Valisime keeli..." ; "Suits sajab korstnaist maha..." ; "Aeg on karm ja ühepoolne Meid..."; Kell viis kui pole lund

  11. Ida-Virumaa rahvakultuuri mänguline muusa - Meeri Amanda / Ene Raudar

    Index Scriptorium Estoniae

    Raudar, Ene

    2015-01-01

    Ida-Virumaa kultuuritöötajate ühisest aktsioonist 2014. a. 26. okt. - kultuuri killavoor Aserist Avinurme, et Eesti Pärandkultuuri aastal pöörata rohkem tähelepanu väikestele rahvamajadele, kus igapäevast rahvakultuuri elus hoitaksegi

  12. Kui tuulispasa poolt pühitud / Ene Läkk

    Index Scriptorium Estoniae

    Läkk, Ene, 1957-

    1998-01-01

    Nõmme keskuses asunud kultuurimälestiseks tunnistatud tornist, mille kevadel lammutas Tallinna Elektrivõrk. Muinsuskaitseinspektsioon nõuab torni taastamist. AS 'Vunder' kavatses sinna rajada kunstigalerii, sidudes torni uusehitisega. Arhitekt Margus Koot arhitektuuribüroost Koot ja Koot. Kommenteerivad Maret Järv Nõmme linnaosavalitsusest, Margus Koot, Andri Ksenofontov jt.

  13. Vene kinopravdast sai prantsuse cinema-verite / Ronald Bergan ; tõlk. Ene Jürna

    Index Scriptorium Estoniae

    Bergan, Ronald

    2004-01-01

    FIPRESCI asepresident meenutab 18. veebruaril 2004.a. surnud prantsuse dokumentalistika suurkuju Jean Rouchi (sünd. 31.05.1917). Lisatud Jaan Ruusi kommentaar Rouchi sidemetest Eesti kineastide Mark Soosaare ja Vladimir Karassev-Orgussaarega

  14. UGROŽENE VRSTE RIBA U SVIJETU: Aphanius farsicus Teimori, Esmaeili i Reichenbacher, 2011 (Cyprinodontidae)

    OpenAIRE

    KEIVANY, Yazdan; Reza Esmaeili, Hamid

    2013-01-01

    Vrsta šaranozupke Aphanius farsicus Teimori (Esmaeili i Reichenbacher, 2011) iz porodice Cyprinodontidae ili endemična farska vrsta nekoć je bila česta u slivu jezera Maharloo, a danas nestaje iz autohtonog staništa. Ova vrsta šaranozupke još se ne nalazi na popisu IUCN-ove Crvene knjige ugroženih vrsta, iako zadovoljava sve kriterije za svrstavanje na spomenuti popis – ograničena joj je distribucija, mrijestilišna područja se razaraju, zagađuje se okoliš i javlja se suša. Ova osjetljiva vrst...

  15. Tammsaare, Kangro ja Vanapagan / Ilse Lehiste ; inglise k. tlk. Ene-Reet Soovik

    Index Scriptorium Estoniae

    Lehiste, Ilse, 1922-2010

    2000-01-01

    Orig.: Tammsaare, Kangro and the Devil. Varem ilmunud: Baltic Literature and Linguistics. Ohio : Association for the Advancement of Baltic Studies, 1973. A. H. Tammsaare romaanist "Põrgupõhja uus Vanapagan" (1939) ning Bernard Kangro romaanist "Taeva võtmed" (1956)

  16. Kriisimissioonidel ei anta üksnes abi, seal õpitakse palju ka ise / Ene Pajula

    Index Scriptorium Estoniae

    Pajula, Ene, 1950-

    2010-01-01

    Lääne-Tallinna keskhaigla kirurgia- ja anestesioloogiakliiniku juhataja, anestesioloog Jaak Talving on üks Eesti kümmekonnast päästemeedikuks koolitatud arstist. Ta on käinud missioonil Indoneesias ja Haitil ning aidanud sisustada Afganistani väikehaiglat

  17. 1-[(Z-1-Bromo-2-(butyldichloro-λ4-tellanylethenyl]cyclohex-1-ene

    Directory of Open Access Journals (Sweden)

    Julio Zukerman-Schpector

    2011-07-01

    Full Text Available The TeIV atom in the title compound, [Te(C4H9(C8H10BrCl2] or C12H19BrCl2Te, is in a distorted ψ-trigonal–bipyramidal geometry, with the lone pair of electrons projected to occupy a position in the equatorial plane, and with the Cl atoms being mutually trans [172.48 (4°]. Close intramolecular [Te...Br = 3.3444 (18 Å] and intermolecular [Te...Cl = 3.675 (3 Å] interactions are observed. The latter lead to centrosymmetric dimers which assemble into layers in the bc plane. The primary connections between layers are of the type C—H...Cl.

  18. Tre tilfaelde af visceral leishmaniasis: det ene hos en HIV-positiv mand

    DEFF Research Database (Denmark)

    Balslev, U; Jonsbo, F; Junge, Jette

    1991-01-01

    Three cases of visceral leishmaniasis (kala-azar) are presented. One of these was in a 43-year-old patient with AIDS who was infected in Southern Spain. Another was in a man aged 25 years infected in West Africa. These cases are the first two adults to be reported in Denmark. The third case...... was an 18 month old previously healthy boy, infected in Southern Spain. The symptomtology, diagnosis and treatment of the disease are discussed and it is stressed that serological diagnostic tests have limited value in HIV positive patients....

  19. Otsusta Eesti Tulevik / Arnold Rüütel, Ene Ergma, Juhan Parts

    Index Scriptorium Estoniae

    Rüütel, Arnold

    2003-01-01

    Ilmunud ka Valgamaalane (2003/Jun/28) lk. 2 ; Sakala (2003/Jun/27) lk. 2 ; Koit (2003/Jun/26) lk. 7 ; Maaleht (2003/Jun/26) lk. 4 ; Põhjarannik (2003/Jun/26) lk. 2 ; Severnoje Poberezhje (2003/Jun/26) lk. 2 ; Vooremaa (2003/Jun/26) lk. 1 ; Vaba Eesti Sõna (2003/Jul/2) lk. 3 ; Tribüün : Euroopa Liidu rahvahääletuse eri (2003/Aug/15) lk. 3. Vabariigi Presidendi, Riigikogu esimehe ja peaministri ühisavalduse tekst

  20. Interviewer behaviour during oral proficiency interviews : a gender perspective / Edith Reemann, Ene Alas, Suliko Liiv

    Index Scriptorium Estoniae

    Reemann, Edith

    2013-01-01

    Analüüsitakse intervjueerija käitumise soolisi iseärasusi inglise keele riigieksami suulise keelepädevustesti läbiviimisel, kas on olemas ühele või teisele sugupoolele omaseid käitumismalle ning kas need võiksid mõjutada intervjueeritavat oma oskuste demonstreerimisel

  1. Artur Lemba - tunglakandja : Ühe suurmehe metaelulugu / Ene Metsjärv ; intervjueerinud Marju Riisikamp

    Index Scriptorium Estoniae

    Metsjärv, Ene, 1943-

    2014-01-01

    Pianist, helilooja ja pedagoog Artur Lemba elust ja loometööst. A. Lemba õpilaste Veera Lensini, Salme Lohuaru (kuni 1950 Andre), Valentine Riivese, Tiiu Targamaa, Villem Reimanni ja Elsa Avessoni ülestähendusi oma õpetajast

  2. Emotsioonid eesti keele käeväljendites / Ene Vainik

    Index Scriptorium Estoniae

    Vainik, Ene, 1964-

    2010-01-01

    Analüüsitakse eesti keele kõnelejate konventsionaalseid teadmisi ja ettekujutusi suhtlusprotsessist, uurides lähemalt leksikaalseid väljendeid, mille üheks komponendiks on viide käele (või selle osale)

  3. Dollar$ & $en$e. Part IV: Measuring the value of people, structural, and customer capital.

    Science.gov (United States)

    Wilkinson, I

    2001-01-01

    In Part I of this series, I introduced the concept of memes (1). Memes are ideas or concepts, the information world equivalent of genes. The goal of this series of articles is to infect you with my memes, so that you will assimilate, translate, and express them. We discovered that no matter what our area of expertise or "-ology," we all are in the information business. Our goal is to be in the wisdom business. We saw that when we convert raw data into wisdom we are moving along a value chain. Each step in the chain adds a different amount of value to the final product: timely, relevant, accurate, and precise knowledge which can then be applied to create the ultimate product in the value chain: wisdom. In Part II of this series, I infected you with a set of memes for measuring the cost of adding value (2). In Part III of this series, I infected you with a new set of memes for measuring the added value of knowledge, i.e., intellectual capital (3). In Part IV of this series, I will infect you with memes for measuring the value of people, structural, and customer capital.

  4. Maakondliku Liivide etluskonkursi võitis gümnasist Marian Heinat / Ene Kallas

    Index Scriptorium Estoniae

    Kallas, Ene

    2008-01-01

    Maakondliku Juhan ja Jakob Liivi etluskonkursi žüriisse kuulusid Ott Aardam, Käthe Pihlak, Piret Rauk, Vaido Luks ja Marili Pärtel. Grand prix' võitis Marian Heimat, laureaatideks tulid Liisa Saaremäel, Maria Pihlak ja Laura Oolup. Eripreemiad said Marii Metsmaa, Ermo Loik, Sander Aavik, Kaimar Nuut ja Priidu Aardam

  5. Asutamisvabadus Euroopa Ühenduse õiguses : kohaldatavus ladina notariaadile / Ene Andresen

    Index Scriptorium Estoniae

    Andresen, Ene, 1977-

    1999-01-01

    Euroopa Ühenduse asutamislepingu kohaldatavusest notariametile, "avaliku võimu teostamise" mõistest ning vastavatest Euroopa Kohtu lahenditest, Eesti notariaaltoimingute vastavusest Euroopa Ühenduse asutamislepingule ning üksikute tegevuste eraldatavuse probleemist

  6. Disainer Maile Grünberg üllatab taas / Ene Läkk

    Index Scriptorium Estoniae

    Läkk, Ene, 1957-

    2011-01-01

    Maile Grünbergi poolt kujundatud kasevineerist mööbliseeria "Veneer MG" Tallinnas Arhitektuuri ja Disainigaleriis 24.11.-14.12.2011. Kaubamärk "Veneer MG" sündis koostöös ettevõttega Aja Nägu OÜ. Mööbel on mõeldud seeriatootmiseks

  7. Solvent Effects in Asymmetric Hetero Diels-Alder and Ene Reactions

    DEFF Research Database (Denmark)

    Johannsen, Mogens; Jørgensen, Karl Anker

    1996-01-01

    The use of polar solvents such as nitromethane or 2-nitropropane leads to a significant improvement of the catalytic properties of a cationic copper-Lewis acid in the hetero Diels-Alder reaction of alkyl glyoxylates with dienes; The scope of a newly developed copper(II)-bisoxazoline catalyst...... for the hetero Diels-Alder reaction is demonstrated by the reaction of different dienes with alkyl glyoxylates in nitroalkane solvents with the formation of the hetero Diels-Alder adduct in high enantiomeric excess as the major product. The synthetic application of the reaction is exemplified by an improved...

  8. Learning Organizations. Studiu exploratoriu asupra Consiliilor Judeţene din România

    Directory of Open Access Journals (Sweden)

    Diana GHIURA

    2012-12-01

    Full Text Available This article is the result of an ample study centered on the concept of learning organizations, more precisely on the degree in which Romanian County Councils are learning organizations. In order to analyze this, a research instrument has been sent to every County Council. This tool has been developed by three Harvard professors: David A. Garvin, Amy C. Edmondson and Francesca Gino who also published, in 2008, an article in Harvard Business Review ‘Is Yours A Learning Organization?’. Their article is fundamental for this study. Approximately 26 out of 41 County Councils participated to the study and the findings are summarized in this article. The results were really interesting and according to them, Romanian County Councils are learning organizations only in terms of leadership that encourages learning. Regarding the other two blocks – learning environment and learning processes, the findings revealed that the County Councils act like learning organizations in a very small degree. As a consequence, the organizations that participated to the study still have many things to improve in order to become learning organizations.

  9. "Juu Jääb" 2009 džässisaarel / Ene Kallas

    Index Scriptorium Estoniae

    Kallas, Ene

    2009-01-01

    Kolmeteistkümnenda tulevikumuusika festivali "Juu Jääb" (peakorraldaja Villu Veski) üritustest Muhu saarel, Vormsil ja Kuressaares. Muhus Nautse külas Mihkli talus toimunud kontsertidest, kus esinesid saksa lauljanna Pascal von Wroblewsky, USA ansambel Napoleon Maddox Quartet, saksofonist Lembit Saarsalu koos Trio X-ga, Ivo Linna koos Olav Ehala, Toivo Undi ja Kaspar Kallustega

  10. Courants du Monde'i programmi toel Prantsusmaal / Asko Tamme ; intervjueerinud Ene Riet

    Index Scriptorium Estoniae

    Tamme, Asko

    2010-01-01

    Intervjuu Tartu Linnaraamatukogu direktori Asko Tammega, kes käis tutvumas Prantsusmaa raamatukogudega, sh Pompidou keskuse raamatukoguga, Rennes'i keskraamatukoguga ning Raamatu ja Lugemise Bretagne'i keskusega (Livre et Lecture en Bretagne)

  11. Intramolecular butenolide allene photocycloadditions and ensuing retro-ene reactions of some photoadducts

    NARCIS (Netherlands)

    Lutteke, G.; Kleinnijenhuis, R.A.; Jacobs, I.; Wrigstedt, P.J.; Correia, A.C.A.; Nieuwenhuizen, R.; Hue, B.T.B.; Goubitz, K.; Peschar, R.; van Maarseveen, J.H.; Hiemstra, H.

    2011-01-01

    This report describes intramolecular [2+2] photocycloadditions of several butenolides with an allenylmethyl substituent at the 5-position. These photosubstrates were prepared from 2-(silyloxy)furans through silver-mediated reactions with allenylmethyl bromides. Two cases were studied where the

  12. The role of trust in business relations in Estonia / Urve Venesaar, Ene Kolbre

    Index Scriptorium Estoniae

    Venesaar, Urve, 1944-

    2004-01-01

    Ärisuhete analüüsi tulemustest lähtuvalt järeldavad autorid, et Eesti ettevõtjad ei ole koondanud oma ärisuhted ainult isiklikule, vaid ka kollektiivsele ja institutsionaalsele usaldusele, mida on mõjutanud ettevõtete kohanemise vajadus tänaseks välja kujunenud Eesti ärikeskkonnas. Tabelid

  13. Mida teevad mõtted, kui nad "mõlguvad"? / Ene Vainik

    Index Scriptorium Estoniae

    Vainik, Ene, 1964-

    2013-01-01

    Kognitiivsest semantikast, eriti kehapõhisuse teooriast lähtudes, uuritakse, milline kehaline kogemus on motiveerinud sõna "mõlkuma" kasutuselevõtmist perioodilisi mõtteprotsesse tähistava verbina

  14. Päevad on laused : [luulekava] / Ene Mihkelson ; koost. Arne Merilai

    Index Scriptorium Estoniae

    Mihkelson, Ene, 1944-2017-

    2005-01-01

    Sisu: "Ühel väga pimedal väga lagedal väljal kus must taevas..." ; "Luuletaja elas pimedas toas ja ootas nägemusi..." ; "Ma lähen ma tulen ma olen ma seisan alati paigal..." ; "Miks meeldib mulle tetraeeder just karjamaal..." ; "Igavene on ilmaköis..." ; "Elu ongi jumalagajätt väljamõeldud..." ; "Kui olin saanud neljaseks Nii väga kiitis..." ; "Meid oli üks tüdruk Me rääkisime..." ; "Olin kaua endaga ma isekeskis õppisin..." ; "Varemed ei põle need vajuvad mulda..." ; "Juustes kasvab valgus..." ; "Küll tahaks vabaneda valeluulest..." ; "Ta armastus on surm..." ; "Minult on küsitud, kas ma saan keerulisemalt ja ma olen öelnud..." ; "Ta haridus oli lünklik, omandatud peamiselt iseõppimise..." ; "Valge kala ujub läbi metsa..." ; "Sinagi kuuled vist kuidas..." ; "Raual on raua kõla..." ; "Need köied ripuvad..." ; "Ärkasin kolmekümne seitsme aastasena..." ; "Üksteise sisse on minek asjadel ja elul..." ; "Mitte metsade vahelt vaid põldude äärest..." ; "Hämarusest tulevad ja hämarusse lähevad näod..." ; "Ta jutustas, mida nägi, ja see oli nii, nagu oleksid kõik..." ; "Mu päevatöö on read mu päevatöö on vari..." ; "Minevik on müüa..." ; "Üks kauge küla tappis ennast ise..." ; "Suur must auk on küla sees..." ; "Rooste on rahulik Rooste..." ; "Mees kes pianisti sõrmeluid ükshaaval murdnud..." ; "Kellele tema oma hinge müüs..." ; "Tuletan sulle meelde et oleme palju..." ; "Sest tema oli barbar mitte päritolu tõttu, vabast tahtest..." ; "Kui veel reagi kirjutad..." ; "Sügavale on taevas mind matnud..." ; "Kes ta siis ikkagi oli: inimene eikuskilt, miks ta ei..." ; "Me läheme ürgsele maale seal pilvede kodu..." ; "Kannan sõelaga aega..." ; "Jumal sina oled näinud mu kujutluste..." ; "Tõstsin kivi mille all elas vähk..." ; "See hingelõpp see võppumine..." ; "Risti alla pandi sinu keha..." ; "Igal ajal ööl on musta värvi..." ; "Vahest elaski kord mees kelle..." ; "Vast ainult üks meil on see päris hull..." ; "Arturi kollased silmad..." ; "Lumi on katnud kinni varemed ja kuusehekk kasvanud..." ; "Kord jäävad ainult jooned ajalukku..." ; "Meie kirjutame luuletusi hõredale õhule..." ; "Ammutamatu on õhtu mil läheme koju..." ; "Väike poiss igavesti narmendavas palitus..." ; "Aadu oli mu klassivend..." ; Kell viis kui pole lund

  15. 78 FR 717 - ENE (Environment Northeast); Greater Boston Real Estate Board; National Consumer Law Center...

    Science.gov (United States)

    2013-01-04

    ... Consumer Law Center; NEPOOL Industrial Customer Coalition; v. Bangor Hydro-Electric Company; Central Maine Power Company; New England Power Company; New Hampshire Transmission LLC; NSTAR Electric Company; Northeast Utilities Service Company; The United Illuminating Company; Unitil Energy Systems, Inc.; Fitchburg...

  16. Collective memory and the politics of experience in Estonian post-Soviet life stories / Ene Kõresaar

    Index Scriptorium Estoniae

    Kõresaar, Ene, 1971-

    2005-01-01

    Elulugude põimumine kultuuritraditsioonide ja suulise traditsiooniga ning nende jutustamine kui poliitilise ajaloo väljendusvorm. Kollektiivse mälu ja poliitika vahelistest seostest kahe Eesti Kultuuriloolisele Arhiivile saadetud Eesti nõukogude aega kajastava eluloo alusel

  17. 1,2-Bis[bis?(methyl?sulfan?yl)methyl?ene]hydrazine

    OpenAIRE

    Driss, Mohamed; Toumi, Meriem; Ben Amor, Fatma; Driss, Ahmed; Boujlel, Khaled

    2008-01-01

    The title compound, C6H12N2S4, was obtained as a by-product (8%) during the reaction of the electrogenerated cyano?methyl anion with phenyl?amine, carbon disulfide and methyl iodide. The mol?ecule, with the exception of 8 H atoms, lies on a crystallographic mirror plane and is arranged around an inversion centre located at the mid-point of the N?N bond.

  18. Developing interviewer proficiency : a self-perception survey / Riin Kont-Kontson, Ene Alas, Suliko Liiv

    Index Scriptorium Estoniae

    Kont-Kontson, Riin

    2013-01-01

    Inglise keele õpetajate hulgas läbi viidud uurimusest, mis näitas, et intervjueerijate arusaam oma kutsestandardist varieerub märkimisväärselt. Probleemide lahendamiseks esitatakse mitmeid koolitusstrateegiaid

  19. Kütiorus on kõrvuti pronksiaeg ja postmodernism / Ene Läkk

    Index Scriptorium Estoniae

    Läkk, Ene, 1957-

    2000-01-01

    Külaskäik Kütioru Avatud Ateljeesse. Peeter ja Leelo Lauritsa tegemistest ja plaanidest seoses ateljeega. Valminud on ateljeemaja ümberehitamise makett. Ehitusmaterjaliks savi. Nõustavad Tartu ökoinsenerid. Sügisel 1999 viibisid Kütiorus Vogue'i ajakirjanikud ja Londoni fotograaf Zanna, et teha Casa Vogue'ile illustreeritud Kütioru Avatud Ateljeed tutvustav lugu. 5 värvilist illustratsiooni

  20. Archaeological excavations on the cemetery of Kodasoo and the settlement site of Lagedi / Gurly Vedru

    Index Scriptorium Estoniae

    Vedru, Gurly, 1970-

    2004-01-01

    Kodasoo kalme puhul oli tegu maa-aluste põletusmatustega. Tehti kindlaks, et Kodasoo kalme hõlmas algselt u. 50-70 m2 suuruse ala. Päästekaevamistel Lagedi asulakohal olid leidude hulgas ülekaalus uusaegne keraamika ja vanimateks leidudeks üksikud viikingiaegsed savinõud

  1. Söötmisega disainitud piim tagab tervisliku toidukraami / Ene Tammsaar ; intervjueerinud Ain Alvela ; kommenteerinud Üllas Jaaska

    Index Scriptorium Estoniae

    Tammsaar, Ene, 1952-

    2014-01-01

    Et farmidest tööstusse minevast toorpiimast saaks toota maitsvaid ja tervislikke tooteid, peab toorpiim olema kõrgeima võimaliku kvaliteediga. Selle tagamiseks on piimatööstused koostöös teadlastega ette võtnud piima koostise disainimise

  2. Ene News April 2003 - Information on energy research (German version); ENET News, April 2003, Nr. 54 deutsch

    Energy Technology Data Exchange (ETDEWEB)

    Wellstein, J.

    2003-07-01

    The April 2003 issue of ENET News, a magazine on energy research in Switzerland published three times a year, covers the topics of energy use in industry, the export of sustainable energy technologies and reviews the Swiss Research Concept for the period 2004 - 2007. Further articles discuss technology transfer from research institutes to industry, technology monitoring, new photovoltaic technologies, new techniques for the recycling of metal, wood energy and the energetic use of biogas from wastewater treatment plants and of drainage water from alpine tunnels. Further, small hydro schemes are described, the hydronics of small heat pump systems are discussed and the efficient use of fossil fuels in combined heat and power / heat pump combinations is examined. Other topics covered include low-energy consumption building, nuclear reactor safety and biomass-based fuels.

  3. Perspectives on the emblems of the nation in contemporary Estonian and Anglo-Welsh poetry / Ene-Reet Soovik

    Index Scriptorium Estoniae

    Soovik, Ene-Reet, 1968-

    2000-01-01

    Kaasaegse eesti luule ja Walesi ingliskeelse luule võrdlemiseks on kasutatud uusi antoloogiaid: Eesti luule antoloogia II (koost. Raivo Kuusk, 1998), Twentieth-century anglo-welsh poetry (ed. Dannie Abse, 1997)

  4. Switchable 1,8-diazabicycloundec-7-ene and 1-hexanol ionic liquid analyzed by liquid ToF-SIMS

    Energy Technology Data Exchange (ETDEWEB)

    Yao, Juan; Zhou, Yufan; Sui, Xiao; Lao, David; Heldebrant, David; Zhu, Zihua; Yu, Xiao-Ying

    2016-06-01

    Switchable ionic liquids (SWIL) play an important role in green chemistry. Due to the nature of SWIL chemistry, such as air sensitivity and pressure and temperature dependence, it is difficult to characterize SWIL using vacuum-based surface techniques. The fully CO2 loaded and none-loaded DBU and hexanol mixtures, a SWIL system, were analyzed in situ by time-of-flight secondary ion mass spectrometry (ToF-SIMS) coupled with the System for Analysis at the Liquid Vacuum Interface (SALVI), respectively. The DBU/Hexanol/CO2 SWIL was injected into the microchannel before liquid SIMS analysis. Bi3+ primary ion beam was used. The positive and negative spectra of the SWIL chemical components are presented. The characteristic peaks m/z 153 (reduced DBU) in the positive mode and m/z 101 (oxidized hexanol) in negative mode were observed. In addition, ion pair peaks including m/z 253, 319, 305, 451 in the positive mode and m/z 145, 223, 257 in the negative mode are first observed using this approach. These results demonstrate that the SALVI microfluidic reactor enables the vacuum-based surface technique (i.e., ToF-SIMS) for in situ characterization of challenging liquid samples such as ionic liquids.

  5. Villa Jaapanis Nagano maakonnas : võtmesõnaks on ökoarhitektuur / Anne Prommik, Ene Läkk

    Index Scriptorium Estoniae

    Prommik, Anne

    1999-01-01

    Tokio lähedal metsa keskel asuv kunstigalerii omanikust tellija villa, kus on ruum kunstiteoste eksponeerimiseks ja korter kahe magamistoa ning loodusvaatega vannitoaga. Maja baaselemendiks on ring. Planeeringus on tõmmatud kindlapiiriline joon avaliku ja eluruumi vahele (machiya-disain). Arhitekt Kazuyo Sejima & Associates.

  6. Öeldakse PÖFF, mõeldakse kino kõigile / intervjueerinud Ene Läkk

    Index Scriptorium Estoniae

    Lokk, Tiina, 1955-

    2011-01-01

    Intervjuu PÖFFi juhi Tiina Lokkiga sellest, kuidas filmifestival laieneb kultuuriüritusest inimõiguste eest võitlejaks maailmas. Tänavu on PÖFFi kavas kuuendat aastat inimõiguste filmiprogramm, sel korral fookuses puuetega inimeste võrdne kohtlemine. Uuringust, millega selgitati, kas ja kui ligipääsetavad on Coca-Cola Plaza ja Solarise kino puuetega inimestele

  7. Aktuaalsed teemad Brüsseli COPA-COGECA töögruppides / Ene Kärner

    Index Scriptorium Estoniae

    Kärner, Ene, 1973-

    2005-01-01

    Euroopa Liidu maaelu arengupoliitikast aastateks 2007-2013, ebasoodsamate piirkondade määratlemisest, riski- ja kriisijuhtimisest põllumajanduses, viina definitsioonist jt probleemidest, mis on arutusel Euroopa Liidu Põllumajandusorganisatsioonide Komitees (COPA) ja Põllumajanduskoostöö Keskkomitees (COGECA)

  8. Notari ametivastutuse alused : [bakalaureusetöö] / Monika Palu ; Tartu Ülikool, õigusteaduskond ; juhendaja: Ene Andresen

    Index Scriptorium Estoniae

    Palu, Monika

    2006-01-01

    Notari õiguslik seisund ja funktsioonid, vastutuse üldiseloomustus, ametivastutuse regulatsiooni areng, notari vastu esitatava kahju hüvitamise nõude võrdlus riigivastutuse seaduse alusel haldusorgani vastu esitatava kahju hüvitamise nõudega

  9. Anti-Human Immunodeficiency Virus Activity of Thiol-Ene Carbosilane Dendrimers and Their Potential Development as a Topical Microbicide.

    Science.gov (United States)

    Sánchez-Rodríguez, Javier; Díaz, Laura; Galán, Marta; Maly, Marek; Gómez, Rafael; Javier de la Mata, F; Jiménez, José L; Muñoz-Fernández, M Angeles

    2015-10-01

    The concept of a "microbicide" was born out of the lack of a vaccine against HIV and the difficulty of women in ensuring the use of preventive prophylaxis by their partners, especially in developing countries. Approaches using polyanionic carbosilane dendrimers have shown promise in the development of new microbicides. We have developed and evaluated two anionic carbosilane dendrimers with sulfonate and carboxylate terminal groups, G2-STE16 and G2-CTE16. Both dendrimers showed high biosafety in human epithelial cell lines derived from the vagina and in primary blood human cells (PBMCs). The dendrimers not only have a greater capacity to block the entry of different X4- and R5-HIV-1 isolates into epithelial cells but also prevent the HIV-1 infection of activated PBMCs. The treatment of epithelial cells with different carbosilane dendrimers did not produce changes in the activation or proliferation of PBMCs or in the expression of CD4, CCR5 or CXCR4. Computational modeling showed significantly higher affinities for the complexes G2-STE16/gp120 and G2-CTE16/gp120. Moreover, no irritation or vaginal lesions were detected in female BALB/c mice after vaginal administration of the dendrimers. Summing up, G2-STE16 and G2-CTE16 are easy to synthesize and compatible with functional groups, and the purification steps are easy and short. Our results have clearly demonstrated that these dendrimers have high potency as a topical microbicide against HIV-1 infection.

  10. Hasarlı Çene Kemiklerinde Abutment Boyunun Dental İmplant Sistemi Üzerindeki Etkileri

    Directory of Open Access Journals (Sweden)

    Sinan KÖSE

    2015-01-01

    Full Text Available The purpose of this study is to investigate the stresses occurred onhard and soft bones after recovering the deficiency caused by totally edentulousand damaged mandible by using abutments with different lengths andprosthesis filling material. Two different damaged parts of 2.5 and 4.5 mm indepth were created with the aid of Solidworks program on the right hand sideof the mandible. Implants were then applied between the foremen channels.Later, the damaged models were recovered by abutment with different lengthsas for the first method. Two models using abutment were, therefore, obtainedfor the damaged parts of 2.5 and 4.5 mm in depth. In the second method, for thesame damaged cases, prosthesis filling material was used to recover deficiencykeeping the abutment length constant. Finally, there were totally obtained 4different damaged models of two fixed with abutment and two fixed with fillingmaterial and 1 undamaged model. At the second step of this study, the stressvalues were obtained on lower jaw bones by using finite element method underthe maximum chewing force and compared to each other. The results showedthat stress data obtained particularly on the soft and hard bones weredistributed more uniformly on the damaged models fixed with prosthesis fillingmaterial compared to the damaged models recovered by abutment. Moreover,the stress values obtained for models fixed with different abutment length wererather high in comparison to those of the filling material application

  11. Loodi Mõisa Looduskeskus : projekt "Turismi arendamise väljakutsed Põhjamaades ja Balti riikides" / Urve Künsar, Ene Sillamaa

    Index Scriptorium Estoniae

    Künsar, Urve

    2016-01-01

    2010. aastal loodud MTÜ Loodi Mõisa Arendus sihiks on Loodi mõisa renoveerimine ning õppe- ja külastuskeskuse rajamine, loodusturismi edendamine, selleks osaletakse ka rahvusvahelises Nordplus Adult programmi poolt toetatud täiskasvanuhariduse projektis koos partnerorganisatsioonidega Leedust, Islandilt ja Rootsist

  12. Maja Cap Ferret's Prantsusmaal : võtmesõnaks on ökoarhitektuur / Anne Prommik, Ene Läkk

    Index Scriptorium Estoniae

    Prommik, Anne

    1999-01-01

    Mere kaldale puude vahele rajatud, saledatele tugisammastele tõstetud ranges stiilis maja on avatud vaid Archachoni rannale. Looduse säilitamiseks on iga maja läbiva puutüve ümber ehitatud metallkonstruktsioon. Tüved läbivad liigutatavat katust kummist 'varruka' kaudu. Projekteerija: Lacaton et Vassal (Bordeaux).

  13. Eugene O'Neill "Pikk päevatee kaob öösse" / Ellu Eik ; interv. Ene Paaver

    Index Scriptorium Estoniae

    Eik, Ellu

    2008-01-01

    Seoses Eesti Draamateatri suvelavastusega "Pikk päevatee kaob öösse" (autor Eugene O'Neill ja lavastaja Merle Karusoo) räägib Lääne-Tallinna Keskhaigla Sõltuvusravikeskuse psühhiaater Ellu Eik sõltuvuse tagamaadest ja mõjudest

  14. Goethe loob väljendi : maailmakirjanduse kultuuripoliitika / David Damrosch ; tõlkinud Ene-Reet Soovik

    Index Scriptorium Estoniae

    Damrosch, David

    2007-01-01

    Johann Wolfgang von Goethe erasekretäri Peter Eckermanni teosest "Vestlused Goethega tema elu viimastel aastatel", Goethe vaadetest kirjandusele, maailmakirjanduse mõisest. Ka kirjanduse tõlkimisest tänapäeval, sinoloog Stephen Oweni vaadetest ja hiina luuletaja Bei Dao luulest. Artikkel sisaldab ka Bei Dao luuletuste näiteid

  15. Educational objectives as the basis for successful coping at school / Leida Talts, Ene Mägi, Helle Sikka... [jt.

    Index Scriptorium Estoniae

    2007-01-01

    Artiklis käsitletakse sotsiaalsete ja eetiliste eesmärkide saavutatust kooliminevate laste rühmades ja esimese kooliaasta lõpus. Uurimus tugineb kolmele küsitlusele, mis viidi läbi ajavahemikus 2002-2004

  16. Marie Underi luule : mõnest tõlkeprobleemist / Ilse Lehiste ; inglise k. tlk. Ene-Reet Soovik

    Index Scriptorium Estoniae

    Lehiste, Ilse, 1922-2010

    2000-01-01

    Orig.: Marie Under's poetry: some problems of translation. Varem ilmunud: Lituanus, 1983, nr. 3. Kaasautor: Rimvydas Shilbajoris. M. Underi luuletuse "Täiskuu" tekst eesti k., tõlge inglise keelde (tlk. I. Lehiste) ja reaalune tõlge inglise keelde, lk. 256-260

  17. E-poliitika ärikeskkonna kujundajana : kaubanduse ja kinnisvaraturu näitel / Ene Kolbre, Urve Venesaar

    Index Scriptorium Estoniae

    Kolbre, Ene, 1946-

    2003-01-01

    Ülevaade infotehnoloogia kasutamise tingimustest äris üldiselt, veidi põhjalikumalt internetikaubanduses ja kinnisvaraturul, toetudes kirjanduse, Eesti ärikeskkonna ja e-poliitika arengu analüüsile

  18. Salme vallateater toob välja muusikalise näitemängu ja suvelavastuse / Ene Kallas

    Index Scriptorium Estoniae

    Kallas, Ene

    2009-01-01

    Salme vallateatril on kavas välja tuua Jara Beneži "Haljal aasal" ainetel Maire Sillavee lavastuses samanimeline muusikaline komöödia ja August Mälgu romaani "Läänemere isandad" ainetel samanimeline suvelavastus, dramatiseerija Sulev Raudsepp, lavastaja Väino Uibo

  19. Tänavune provintsiteatripäev soovis Koidulaulikule õnne sünnipäevaks / Ene Kallas

    Index Scriptorium Estoniae

    Kallas, Ene

    2008-01-01

    Muljeid Saaremaa 11. provintsiteatripäevalt, mis pühendatud Lydia Koidula 165. sünniaastapäevale. Kuus näiteseltskonda esitas Koidula näidendid "Kosjakased", "Säärane mulk ehk Sada vakka tangusoola", "Saaremaa onupoeg" ja Tõnis Kipperi versiooni näidendist "Kosjaviinad" ning luulekompositsiooni "Mu isamaa on minu arm"

  20. Towards a biocompatible artificial lung: Covalent functionalization of poly(4-methylpent-1-ene (TPX with cRGD pentapeptide

    Directory of Open Access Journals (Sweden)

    Lena Möller

    2013-02-01

    Full Text Available Covalent multistep coating of poly(methylpentene, the membrane material in lung ventilators, by using a copper-free “click” approach with a modified cyclic RGD peptide, leads to a highly biocompatible poly(methylpentene surface. The resulting modified membrane preserves the required excellent gas-flow properties while being densely seeded with lung endothelial cells.

  1. Valimiskomisjonide otsuste ja toimingute vaidlustamine : [bakalaureusetöö] / Ene Soop ; Õigusinstituut ; juhendaja: Enn Markvart

    Index Scriptorium Estoniae

    Soop, Ene, 1974-

    1998-01-01

    Avalduste ja kaebuste esitamine, tähtajad, valimiskaebused konstitutsioonikohtus, kaebuste lahendamise erinevad viisid ja tähtajad, võimalikud parandused ja muudatused, keelenõudega seonduvad protestid.Vt. ka Õigusinstituudi toimetised (1998) nr. 1, lk. 10-11

  2. Eestimaa saksa tudengite silma läbi ehk Keelekümblus preisi moodi / koostanud Heli Mattisen, Ene Lokk

    Index Scriptorium Estoniae

    2001-01-01

    Vastavalt TPÜ Keelekeskuse ja Münsteri ülikooli vahel sõlmitud lepingule toimuvad TPÜ-s eesti keele kursused saksa tudengitele. Artikkel koosneb katkenditest, mis pärinevad saksa tudengite Hannes Böttcheri, Martina Meieri, Gerhard Locki, Anke Helbichi ja Karen Meieri kirjatöödest teemal "Kui ma teeksin tõetruu filmi Eestist, siis mida ma näeksin"

  3. Eesti viipekeele kasutamise õiguse tagamine Eestis : [magistritöö] / Hendrick Rang ; Tartu Ülikool, õigusteaduskond ; juhendaja: Ene Andresen

    Index Scriptorium Estoniae

    Rang, Hendrick

    2014-01-01

    Kurtusest ja viipekeelest, viipekeele kui keele reguleerimisest, viipekeele kui puuet kompenseeriva suhtlusabivahendi reguleerimisest rahvusvahelises õiguses, viipekeele õiguslikust seisundist Eestis

  4. Case note: Gerechtshof 's-Hertogenbosch (ABP partnerpensioen ene partner wordt verminderd met aan andere partner toekomend ABP bijzonder partnerpensioen)

    NARCIS (Netherlands)

    Reinhartz, B.E.

    2009-01-01

    De man is gehuwd geweest met mevrouw X tussen 1962 en 1976. Tussentijds heeft de man van 1986 tot 1990 samengewoond met mevrouw Z. In 1988 is er een samenlevingsovereenkomst gesloten tussen de man en vrouw Z. Tussen 1991 en 1995 hebben de man en mevrouw Z weer samengewoond. In 1994 heeft de man het

  5. 17α-Acetoxy-11β-hydroxy-6α-methylpregn-4-ene-3,20-dione

    Directory of Open Access Journals (Sweden)

    Sammer Yousuf

    2012-07-01

    Full Text Available The title compound, C24H34O5, a fungal-transformed metabolite of the injectable contraceptive medroxyprogesterone acetate, consists of four fused rings (A, B, C and D; steroid labelling. Ring A exists in a half-chair conformation while trans-fused rings B and C adopt chair conformations. The five-membered ring D adopts an envelope conformation with the C atom bound to the methyl group at the flap. In the crystal, adjacent molecules are linked by O—H...O and C—H...O hydrogen bonds, forming infinite chains along the a axis.

  6. Crystal structure of (E)-N-[(E)-3-(4-meth-oxy-phen-yl)allyl-idene]naphthalen-1-amine.

    Science.gov (United States)

    Lee, Jae Kyun; Cha, Joo Hwan; Cho, Yong Seo; Min, Sun-Joon; Lee, Joon Kyun

    2014-11-01

    In the title compound, C20H17NO, the dihedral angle between the mean planes of the 4-meth-oxy-phenyl ring and the naphthalene ring is 69.50 (7)°. The meth-oxy group is almost coplanar with the benzene ring to which it is connected [Cb-Cb-Om-Cm torsion angle of -7.9 (2)°; b = benzene and m = meth-oxy] and the imine group displays a C-C-N=C torsion angle is -57.2 (2)°. The imine (C=N) group has an E conformation. In the crystal, weak π-π inter-actions between the benzene rings [centroid-centroid distance = 3.7781 (10) Å] are observed.

  7. Literaatidest ja Eestimaa Kirjanduse Ühingu algusaastatest: õpetlased, entusiastid ja miljöö / Ene Hiio

    Index Scriptorium Estoniae

    Hiio, Ene

    2013-01-01

    Literaatide seisusest ja aukodaniku seisusest. Tallinna kubermangugümnaasiumi ja toomkooli haritlaste sõpruskondadest. Esimesest avalikust raamatukogust. Eestimaa Kirjanduse Ühingu eestvedajatest. Ühingu erinevatest nimekujudest. Asutajaliikmete nimekiri

  8. Rootsilik lapsepõlv ängi ja päikesepaiste vahel / Ene-Reet Soovik

    Index Scriptorium Estoniae

    Soovik, Ene-Reet, 1968-

    2007-01-01

    Arvustus: Tranströmer, Tomas. Suur on mõistatus ; Mälestused seiravad mind / rootsi keelest tõlkinud Tõnis Arnover ja Lennart-Hans Jürgenson ; järelsõna: Tõnis Arnover. Tallinn : Kultuurileht, 2007. (Loomingu raamatukogu ; 27)

  9. Uroboros : [aus "Uroboros"] : [luuletused] / Ene Mihkelson ; tlk. Irja Grönholm ja Gisbert Jänicke

    Index Scriptorium Estoniae

    Mihkelson, Ene, 1944-2017-

    2006-01-01

    Sisu: Uroboros : "In der Nacht fragt heimlich jemand Wie heisst du..." ; "Am Meer stand ein Mann mit einer Sense..." ; "An dem Tag als es Kugeln regnete..." ; "Zum Zeitvertrieb ersteigerte ich mir auf einer Auktion ein paar..." ; "Sie hat sich mit der Zeit eingelassen und daraus..." ; Das Feld ; "Mutter ist wieder bei mir obgleich..." ; "Wir waren ein Mädchen Wir redeten..." ; "Als ich vier Jahre alt wurde Da wurde so viel..." ; "Wer ist dein Vater Der Fremde aus der Ferne..." ; "Das letzte Wort Das letzte Das letzte Das allein...". Orig.: Uroboros : "Öö aegu keegi küsib sala Mis su nimi on..." ; "Mere ees seisis vikatiga mees..." ; "Sel päeval kui sadas kuulivihma..." ; "Ajaviiteks ostsin enampakkumisel sõpru..." ; "Ta ajaga läinud on sohki ja sellest..." ; Põld ; "Mu ema on jälle mu juures kuigi..." ; "Meid oli üks türduk Me rääkisime..." ; "Kui olin saanud neljaseks Nii väga kiitis..." ; "Kes on su isa Kaugelt tulnud võõras..." ; "Viimast sõna Viimast Viimast Ainu..."

  10. Ettevõtte strateegia ja kinnisvara väärtus / Veronika Ilsjan, Ene Kolbre, Signe Liiv

    Index Scriptorium Estoniae

    Ilsjan, Veronika

    2003-01-01

    Ettevõtte strateegia ja kinnisvarastrateegia ühendamine on kahesuunaline protsess - ettevõtte strateegia peab sisaldama kinnisvaraga arvestamist ja kinnisvara juhtimine lähtuma ettevõtte strateegiast. Tabel. Joonis

  11. Synthesis of 1-Methyl-3-oxo-7-oxabicyclo[2.2.1]hept-5-ene-2-carboxylic Acid Methyl Ester

    Directory of Open Access Journals (Sweden)

    Gil Valdo José da Silva

    2005-11-01

    Full Text Available A simple and efficient method for the preparation of 1-methyl-3-oxo-7- oxabicyclo[2.2.1]hept-5-en-2-carboxylic acid methyl ester (1 is described. The first step is a highly regioselective Diels-Alder reaction between 2-methylfuran and methyl-3-bromo- propiolate. A remarkably difficult ketal hydrolysis reaction was effected by treatment with HCl, a simple reagent that was shown to be more efficient, in this case, than commonly used more elaborate methods.

  12. "WhatsApp"ening in orthopedic care: a concise report from a 300-bedded tertiary care teaching center.

    Science.gov (United States)

    Khanna, Vishesh; Sambandam, Senthil N; Gul, Arif; Mounasamy, Varatharaj

    2015-07-01

    Smartphones have emerged as essential tools providing assistance in patient care, monitoring, rehabilitation, communication, diagnosis, teaching, research and reference. Among innumerable communication apps, WhatsApp has been widely popular and cost effective. The aim of our study was to report the impact of introduction of a smartphone app "WhatsApp" as an intradepartmental communication tool on (1) awareness of patient-related information, (2) efficiency of the handover process and (3) duration of traditional morning handovers among orthopedic residents in a 300-bedded tertiary care teaching center. Written handovers and paging used for communication at our center led to occasional inefficiencies among residents. Widespread use, low cost, availability and double password protection (phone lock and WhatsApp lock) made WhatsApp's group conversation feature an ideal tool for intradepartmental patient-related communication. Twenty-five consecutive admissions before and after WhatsApp (BW, AW) were included in the study. Eight orthopedic residents attempted fifty randomly arranged questions based on the twenty-five patients in each study period. A null hypothesis that introduction of WhatsApp group would neither increase the awareness of patient-related information nor improve the efficiency of the handovers among residents was assumed. A significant improvement observed in scores obtained by residents in the AW group led to rejection of the null hypothesis. The residents also reported swifter and efficient handovers after the introduction of WhatsApp. Our results indicate that the introduction of a smartphone app "WhatsApp" as an intradepartmental communication tool can bring about an improvement in patient-related awareness, communication and handovers among orthopedic residents.

  13. "Poistelaulu" üks hõbe jäi Pärnusse

    Index Scriptorium Estoniae

    2005-01-01

    Konkursi "Poistelaul 2005" lõppvoorus 17. apr. Pärnu kontserdimajas saavutas noormeeste vanuserühmas teise koha Toomas Volli laulukooli õpilane Oliver Timmusk, võistulaulmise lõppvoorus osalesid veel pärnakad Karl Paisuots, Oskar Samuel Rebane ja Carl Väljaäär. Üldvõidu pälvis Türi Gümnaasiumi õpilane Mikk Dede

  14. Tööstus modernismi mõjutajana / Epp Lankots

    Index Scriptorium Estoniae

    Lankots, Epp, 1976-

    2008-01-01

    Raamatust "Industry and modernism : companies, architecture and identity in the Nordic and Baltic countries during the high-industrial period" (Helsinki : Finnish Literature Society, 2007). Toimetaja Anja Kervanto Nevanlinna. Mart Kalm kirjutab Eesti maapiirkondade industrialiseerimisest ning KEK-ide arhitektuurist

  15. Louis Kahn. Ajaloost ajatust otsides / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2006-01-01

    6.-7. okt. 2006. a. toimuvad Kuressaares esimesed Kahni päevad. Eestist pärit ameerika arhitekti Louis Isadore Kahni (1901-1974) eluloolisi andmeid ja loomingust. Kommenteerivad Mart Kalm, Raine Karp, Veljo Kaasik, Toivo Tammik

  16. Eesti parim puitehitis on Aarna loomalautade kompleks Põlvamaal / Helve Laasik

    Index Scriptorium Estoniae

    Laasik, Helve, 1958-

    2003-01-01

    Eesti Metsatööstuse Liidu ja Puuinfo korraldatud arhitektuurivõistluse parima puitehitise leidmiseks tulemused. Hindas žürii koosseisus Veljo Kaasik, Toomas Aakre, Mart Kalm, Urmas Elmik ja Märt Riistop

  17. Teenager vaatab tagasi / Karin Hallas

    Index Scriptorium Estoniae

    Hallas, Karin, 1957-

    2000-01-01

    Eesti Arhitektuurimuuseumi asutamisest ja kümneaastasest tegevusest. Arhitektuurimuuseum asutati 1. I 1991. a. Initsiatiivgruppi kuulusid Karin Hallas (tulevane direktor), Krista Kodres ja Mart Kalm. 7. VI 1996. a. avanäitus Rotermanni soolalaos

  18. Raamat "Raidmets" = "Raidmets" book

    Index Scriptorium Estoniae

    2012-01-01

    Tutvustus: Toivo Raidmets / koostanud Toivo Raidmets ; toimetanud Leele Välja ; tekstid: Krista Kodres, Sirje Helme, Harry Liivrand, Mart Kalm. Tallinn : Eesti Kunstiakadeemia, 2011. Loetletud Toivo Raidmetsa tehtud töid

  19. DOCOMOMO cream. Modernismi valitud palad / Tiina Tammet

    Index Scriptorium Estoniae

    Tammet, Tiina, 1971-

    2000-01-01

    Raamatust:The modern movement in architecture : selections from the DOCOMOMO registers. Toimetajad Dennis Sharp ja Chaterine Cooke. Rotterdam : Publishers, 2000. Eesti funktsionalismist on teinud kokkuvõtte Piret Lindpere ja Mart Kalm

  20. Metsameeste muhe kontor / Liivi Tamm

    Index Scriptorium Estoniae

    Tamm, Liivi, 1978-

    2014-01-01

    Riigimetsa Majandamise Keskuse (RMK) kontoriruumide sisekujundus arhitektuuribüroolt Studio-3 (Andres Põime, Liisa Põime, Reet Põime, Anu Põime, Kristi Prinzmann, Lauri Kalm, Lauri Vaimel). Konverentsisaalis taastati Dolores Hoffmanni vitraažid

  1. Plaadid / Margit Tõnson

    Index Scriptorium Estoniae

    Tõnson, Margit, 1978-

    2004-01-01

    Uutest heliplaatidest Embrace "Out of Nothing", Arrested Development "Among The Trees", Kalm, Kristjan "Generalissimus", Bossacucanova "Uma batida diferente", Adams, Bryan "Room Service", "Kasabian", Korn "Greatest Hits. Vol 1", Papa Roach "Getting Away With Murder"

  2. Popnädal : popuudised. Pet Shop Boys - Release. Music from Vanilla Sky. Billboard Top 20 20. aprill 2002 edetabel / Aivar Meos

    Index Scriptorium Estoniae

    Meos, Aivar

    2002-01-01

    Moby uuest singlist "We Are All Made Of Stars" albumilt "18". Eesti sõltumatu tantsulise elektroonika plaadifirma Umblu saadab turule kolmanda väljalaske: koosluse Tallinn 73 meets Kalm split-albumi "Autopiloot". Filmimuusika heliplaatidest.

  3. Ilmus eesti funktsionalistliku arhitektuuri reisijuht

    Index Scriptorium Estoniae

    1998-01-01

    Tallinnas 1998. a. ilmunud Mart Kalmu eesti 1930. aastate arhitektuuri tutvustav 'Eesti funktsionalism/Functionalism in Estonia. Reisijuht' (DOCOMOMO-Eesti). Kujundaja Andres Tali. Mart Kalm DOCOMOMO Eesti grupi peakoordinaatorina

  4. Tallinna 20. sajandi arhitektuurigiid / Triin Ojari

    Index Scriptorium Estoniae

    Ojari, Triin, 1974-

    2000-01-01

    Raamatust: Hallas, Karin, Kodres, Krista, Kalm, Mart. 20th century architecture in Tallinn : architectural guide. Tallinn : Eesti Arhitektuurimuuseum, 2000. Kaasautorid Andres Kurg, Triin Ojari, tõlkija Krista Mits, kujundaja Ivar Sakk

  5. Vananev tööjõud - eelis või puudus? / Urve Vilk

    Index Scriptorium Estoniae

    Vilk, Urve

    2011-01-01

    AS EMT juhatuse liige Valdo Kalm, Baltic Restaurants Estonia ASi personaliosakonna vanemspetsialist Jaana Šmidt ning OÜ Robinson Restoranid tootmis- ja teenindusjuht Rita Veskimeister töötajate vanusest oma ettevõtetes

  6. CCDC 889630: Experimental Crystal Structure Determination : Chloro-(eta^2^-pent-2-ene-1,5-diylbis(di-t-butylphosphine))-rhodium

    KAUST Repository

    Hayashi, Yukiko

    2013-01-01

    An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

  7. Rahapesu ja terrorismi rahastamise tõkestamise seaduse kohaldamise probleemid notarite ametitegevuses : [magistritöö] / Veronika Aunpu ; Tartu Ülikool, õigusteaduskond ; juhendaja: Ene Andresen

    Index Scriptorium Estoniae

    Aunpu, Veronika

    2009-01-01

    Notarite kaasamisest rahapesu ja terrorismi rahastamise tõkestamiseks, tugevdatud hoolsusmeetmete kohaldamise vajalikkusele osundavatest riskidest notari ametitegevuses, notari kohustustest rahapesu või terrorismi rahastamise kahtlusega tehingute korral

  8. Üldine kaubandus (toidukaupade turg) : toidukaupade väliskaubanduse bilanss [2003-2004] / Õrne Pill, Liia Laanes, Ene Maadvere

    Index Scriptorium Estoniae

    Pill, Õrne

    2005-01-01

    Ilmunud ka: Agriculture and the development of rural life : overview 2004/2005. - Tallinn, 2005, lk. 55-65. Põllumajandussaaduste ekspordimaht kasvas eelmise aasta sama perioodiga võrreldes 5,1% ning impordimaht vähenes 1,8%, kaubandusbilansi negatiivne saldo moodustas 4507,7 mln krooni. Diagrammid

  9. Riigisaladus kui informatsioonivabaduse piirang ja sellele juurdepääs : [bakalaureusetöö] / Ene Lehiste ; Tartu Ülikool, õigusteaduskond ; juhendaja: Kalle Merusk

    Index Scriptorium Estoniae

    Lehiste, Ene

    2001-01-01

    Salastatud info kui infovabaduse piirang, riigisaladuse mõiste kehtivas õiguses, salastatud teabe kaitse meetodid, riigisaladuse juurdepääsu alused, julgeolekukontrolli läbiviimine (NATO-s, WEU-s)

  10. Üks kaame naine väikses kambris ... : [luuletused] / Gyula Illyés ; tlk. Sander Liivak, Ene Asu-Õunas

    Index Scriptorium Estoniae

    Illyés, Gyula

    2004-01-01

    Sisu: Üks kaame naine väikses kambris ... ; Sa ei saa pageda ; Purukslöödud vägi ; Mäe otsas puhkan ... ; Kodumaa kõrgel ; Magav naine ; Doleo, ergo surm ; Bart̤k ; Üks lause vägivallast ; Jalutuskäik varjuga ; Tuuline öö ; Liginev külm ; Viltune puri ; Näod ; Aovalgus ; Korraloomine ; Värdjate sünd ; Charoni vene ; Tõusul ; Leegihingaja ; Lähenev vaikus ; Teekond videvikul ; Pärg. Andmeid autori kohta lk. 87-88

  11. Planning and acceptance of catenaries with reference to the High-Speed Energy TSI; Planung und Abnahme von Oberleitungen unter Bezugnahme auf die TSI ENE HGS

    Energy Technology Data Exchange (ETDEWEB)

    Schnadhorst, Kai [Balfour Beatty Rail GmbH, Essen (Germany); Tessun, Heinz [Balfour Beatty Rail GmbH, Muenchen (Germany)

    2011-07-15

    It was in May 2002 that the first-ever Technical Specification for Interoperability (TSI) of the ''energy'' subsystem with validity throughout the European Union was adopted. In March 2008, this was then repealed in conjunction with the adoption of a revised TSI. The new decision has brought fresh movement into this field. (orig.)

  12. Kogesime suurepärast meeskonnatööd / Liisi Lembinen, Elena Sipria-Mironov ; intervjueerinud Õnne Mets, Ene Riet

    Index Scriptorium Estoniae

    Lembinen, Liisi

    2014-01-01

    Tartu Ülikooli Raamatukogu osales EOD-projektis 2009. aasta maist kuni 2014. aasta aprillini. Kuidas projekt toimis ja mida saavutati, vastavad direktori kt Liisi Lembinen ja projektijuht Elena Sipria-Mironov

  13. 40-Godišnjica institucije Cambridge Crystallographic Data Centre posvećene pohranjivanju podataka o molekularnim i kristalnim strukturama -

    Directory of Open Access Journals (Sweden)

    Molčanov, K.

    2006-06-01

    Full Text Available The article is dedicated to 40th anniversary of The Cambridge Crystallographic Data Centre (CCDC, the world-known centre (http://www.ccdc.cam.ac.uk responsible for deposition and control of crystallographic data, including atomic coordinates that define the three-dimensional structures of organic molecules and metal complexes containing organic ligands. Cambride Structural Database (CSD, one among the first established electronic databases, nowadays is the most significant crystallographic database in the world. CSD has about 400,000 deposited structures. The use of the extensive database, which is growing rapidly, needs support of efficient and sophisticated software for searching, analysing and visualising structural data. The seminal role of CSD in the research related to crystallography, chemistry, material sciences, solid state physics and chemistry, life sciences, pharmacology, and in particular in drug design, has been documented in more than 1300 scientific papers. The important issues of CCDC are the accuracy of deposited data and development of software that enables a wide variety of applications. Such demanding project requires higly competent team of experts; thus the article brings into focus the scientific approach of the team based on the long tradition in crystallography, modelling and informatics. The article is not dedicated to 40th anniversary of the centre only, but it also reveals how Cambridge Structural Database can be used in the research and teaching. The use of electronic media and computer graphics makes “data mining" very efficient and useful but also esthetically appealing due to the molecular architecture. At the Rudjer Bošković Institute, Zagreb, Croatia there is The National Affiliated Centre of Cambridge Crystallographic Data Centre responsible for communication and dissemination of CSD in Croatia, Slovenia and Macedonia. The use of CSD is illustrated by two examples performed and published by the presenting authors: a the analysis of the less-common hydrogen bonds with the ester oxygen atom as a proton acceptor, and b topological analysis of tubular assemblies of macrocyclic polythianes extensively described in the references 24 and 28.

  14. Ove rd ep en d en ceon wood-f ue l as a so u rc e of ho use hold ene ...

    African Journals Online (AJOL)

    2005-06-23

    Jun 23, 2005 ... model? The right research question being,. How well does the model fit the reality, repre- sented by the data? (ii) Specious covariates are commonly entered as significant because of compounded Type I er- ror effects. (iii)You cannot assess the uncertainty of your se- lected model and of competing models.

  15. The complexation of a novel squaric bis(thiosemicarbazone); 3,4-bis{[(aminothioxomethyl)amino]azamethylene}cyclobut-ene-1,2-diol

    Science.gov (United States)

    Seleem, H. S.; Ramadan, A. A. T.; Taha, A.; Eid, M. F.; Samy, F.

    2011-03-01

    A novel chelating agent (Sqtsc; H 4L) bearing both hard and soft donor atoms was synthesized by the condensation of squaric acid with thiosemicarbazide. The ligand has two symmetrical sets of donor atoms (SNO), therefore, it was allowed to react with the metal ions at the mole ratio 2:1 (M:L). Mono- and bi-nuclear chelates were obtained in which the ligand showed a variety of modes of bonding viz. (OO) 2-, (SNNS) 2- and (SNO) 2- per each metal ion supporting the ambidentate and flexidentate characters of the ligand. The mode of bonding and basicity of the ligand depend mainly on the type of the metal cation and its counter anion. All the obtained complexes have the preferable O h-geometry except the VO II-complex ( 7) which has also the preferable square pyramid geometry. Structural elucidation was achieved via elemental and spectral data.

  16. VIP EE, Ms Ene Ergma, President of the Estonia Parliament (Riigikogu), Republic of Estonia (attending the 3rd World Conference of Speakers of Parliament in Geneva)

    CERN Multimedia

    2010-01-01

    She toured the LHC superconducting magnet test hall with Andrzej Siemko, leader of the machine protection and electrical integrity group in the technology department, and also saw the CERN Control Centre, the CMS experiment and the LHC tunnel.

  17. Factores asociados a Malaria en el Distrito de Llochegua, Valle de los Ríos Apurimac y Ene, Provincia de La Mar, Ayacucho - Perú - 2003.

    Directory of Open Access Journals (Sweden)

    Ricardo Carreño Escobedo

    2009-07-01

    Full Text Available Objetivo: Estimar los factores asociados para enfermar de Malaria en Llochegua - Ayacucho - Perú en el año 2003. Material y métodos: Estudio caso-control, randomizado, pareado por edad y sexo, considerando 95% de significancia, error beta 0,05 y una razón 1:1, se estimó una muestra de 55 casos. Se estudiaron los siguientes factores de riesgo: número de individuos en la casa, limpieza fuera de la casa, baño diario, uso de mosquitero, uso adecuado de mosquitero, exposición al vector entre las 18.00 y 21.00 horas y distancia de la casa a 200 m de cualquier fuente de agua estancada. Resultados: El uso adecuado de mosquitero (OR= 0,116; IC 95% 0,033-0,342, distancia de casa a 200 m o más de una fuente de agua estancada (OR= 0,112; IC 95%: 0,031 - 0,334, resultaron factores protectores. La regresión logística mostró: los que usan adecuadamente mosquitero tienen 85% menos chance de enfermar de malaria en comparación con los que no (p=0,001; IC 95%: 0,052 - 0,441, independiente de la distancia a la fuente de agua estancada, limpieza dentro casa y uso de mosquitero. Los que viven cerca de aguas estancadas tienen 6,6 veces más probabilidad de hacer malaria (p=0,001; IC 95%: 2,247-19,506; independientemente del uso del mosquitero, limpieza dentro casa y uso adecuado de mosquitero. Conclusiones: La distancia a la fuente de agua estancada ≥ 200 metros y el uso apropiado de mosquiteros son factores predictivos protectores de evitar enfermedad. (Rev Med Hered 2009;20:139-145.

  18. tert-Butyl 1-(Furan-2-yl-4-oxo-2,3,7-triazaspiro[4.5]dec-1-ene-7-carboxylate

    Directory of Open Access Journals (Sweden)

    Balladka Kunhanna Sarojini

    2012-05-01

    Full Text Available A simple and novel route for synthesis of new spirocyclic compound is developed. The present work involves condensation of ethyl nipecotate with 2-furaldehyde followed by the MnO2 oxidation to give the β-keto ester which upon reaction with hydrazine hydrate gives the spiro pyrazolone.

  19. Inventaire et identification des plantes mellifères de la zone soudano-guinéene d'altitude de l'ouest Cameroun

    Directory of Open Access Journals (Sweden)

    Ngouo, LV.

    2004-01-01

    Full Text Available Inventory and Identification of Melliferous Plants of the Soudano-guinean Western Highlands of Cameroon. Between November 2000 and 2001, an inventory of major important major melliferous plants of the soudano-guinean Western highlands of Cameroon (Latitude North 5° 21.45' - 5° 35.44' and Longitude East 10° 04.72' - 10° 26.24' was carried out. A total of 78 melliferous plants belonging to 33 families were identified. In terms of the number of plants, the mostrepresented species were Asteraceae (12.9%; Solanaceae (8.6%; Euphorbiaceae (7.6%; Myrtaceae and Malvaceae (6.4% respectively in decreasing order. When domestication was considered, cultivated species had the highest proportion (64% of the total melliferous plants identified. Perennial cultivated species were nearly two third (63.5% more numerous than annual cultivated species (36.5%. Melliferous species flowering in the rainy season (March- November were more represented (69.2 % compared to those flowering in the dry season (16.7% and throughout the year (14.1%. Melliferous plant flowers showed a great diversity of colours with, however, a predominance of white colour (36%. Plants producing pollen were more numerous (41% as compared to production of nectar (23% and of both nectar/ pollen (16%.

  20. Ohutu või mürgine mõnu? / Monica Raud ; kommenteerinud Märt Lillesiim, Ene Kupper, Enno Tammer, Tiina Tammer

    Index Scriptorium Estoniae

    Raud, Monica, 1981-

    2014-01-01

    Vestlusest Tervise Arengu Instituudi eksperdi Tiiu Härmi, TÜ kopsukliiniku arst-õppejõu Ülle Ani ja sotsiaalministeeriumi keskkonnatervise ja kemikaaliohutuse juhi Aive Tellinguga e-sigarettide mõjust tervisele

  1. Eesti pensionisüsteem Euroopa Liidu ühiste pensionimärkide valguses / Lauri Leppik, Ene-Margit Tiit, Andres Võrk

    Index Scriptorium Estoniae

    Leppik, Lauri, 1964-

    2004-01-01

    Eesti pensionisüsteemi probleemid on senistest liikmesriikidest erinevad: pensionisüsteemi jätkusuutlikkus on hea ja pensionäride vaesusrisk ei ole kõrge, kuid asendusmäär on suhteliselt madal. See tähendab, et ei suudeta tagada EL-i pensionieesmärki, mis taotleb, et inimesel oleks pensionile jäädes võimalk säilitada oma varasem elatustase. Diagramm. Tabelid. Graafikud

  2. Stereochemistry of the epoxidation of bicyclo[2.2.1]hept-2-ene and its 7-syn-substituted derivatives. A DFT study

    Directory of Open Access Journals (Sweden)

    Sergiy I. Okovytyy

    2014-12-01

    Full Text Available The stereochemical aspects of epoxidation of norbornene and its 7-syn-substituted derivatives by performic acid were investigated. Geometry and thermodynamic parameters of transition states and prereactive complexes were computed at the UBHandHLYP/6-31G(d level of theory. It is shown that the transition states have a pronounced biradical character and a nearly coplanar orientation of the C=C bond and the molecule of performic acid. Transition state analysis revealed that, in the case of the syn-7-hydroxy derivative, the preference for the exo-approach of the oxidant can be explained by the stabilization of transition state with hydrogen bonding. In contrast, a chlorine atom or a methyl group at the 7-syn position facilitated the formation of endo-epoxides due to steric repulsion between the substituent and the oxidant.

  3. (E-2,2′-[3-(2-Nitrophenylprop-2-ene-1,1-diyl]bis(3-hydroxycyclohex-2-en-1-one

    Directory of Open Access Journals (Sweden)

    Joo Hwan Cha

    2012-01-01

    Full Text Available In the title compound, C21H21NO6, each of the cyclohexenone rings adopts a half-chair conformation. Each of the pairs of hydroxy and carbonyl O atoms are oriented to allow for the formation of intramolecular O—H...O hydrogen bonds, which are typical of xanthene derivatives.

  4. Cobalt Alloy Implant Debris Induces Inflammation and Bone Loss Primarily through Danger Signaling, Not TLR4 Activation: Implications for DAMP-ening Implant Related Inflammation.

    Science.gov (United States)

    Samelko, Lauryn; Landgraeber, Stefan; McAllister, Kyron; Jacobs, Joshua; Hallab, Nadim James

    2016-01-01

    Cobalt alloy debris has been implicated as causative in the early failure of some designs of current total joint implants. The ability of implant debris to cause excessive inflammation via danger signaling (NLRP3 inflammasome) vs. pathogen associated pattern recognition receptors (e.g. Toll-like receptors; TLRs) remains controversial. Recently, specific non-conserved histidines on human TLR4 have been shown activated by cobalt and nickel ions in solution. However, whether this TLR activation is directly or indirectly an effect of metals or secondary endogenous alarmins (danger-associated molecular patterns, DAMPs) elicited by danger signaling, remains unknown and contentious. Our study indicates that in both a human macrophage cell line (THP-1) and primary human macrophages, as well as an in vivo murine model of inflammatory osteolysis, that Cobalt-alloy particle induced NLRP3 inflammasome danger signaling inflammatory responses were highly dominant relative to TLR4 activation, as measured respectively by IL-1β or TNF-α, IL-6, IL-10, tissue histology and quantitative bone loss measurement. Despite the lack of metal binding histidines H456 and H458 in murine TLR4, murine calvaria challenge with Cobalt alloy particles induced significant macrophage driven in vivo inflammation and bone loss inflammatory osteolysis, whereas LPS calvaria challenge alone did not. Additionally, no significant increase (p500pg/mL). Therefore, not only do the results of this investigation support Cobalt alloy danger signaling induced inflammation, but under normal homeostasis low levels of hematogenous PAMPs (<2pg/mL) from Gram-negative bacteria, seem to have negligible contribution to the danger signaling responses elicited by Cobalt alloy metal implant debris. This suggests the unique nature of Cobalt alloy particle bioreactivity is strong enough to illicit danger signaling that secondarily activate concomitant TLR activation, and may in part explain Cobalt particulate associated inflammatory and toxicity-like reactions of specific orthopedic implants.

  5. Vahistatute võrdne kohtlemine Eesti vanglates ja arestimajades : [magistritöö] / Sigrid Nurm ; Tartu Ülikool, õigusteaduskond ; juhendajad: Ene Andresen, Berit Aaviksoo

    Index Scriptorium Estoniae

    Nurm, Sigrid, 1988-

    2013-01-01

    Võrdse kohtlemise põhiõigusest, kinnipidamistingimustest ja riietusest, keelatud esemetest, kambrivälisest tegevusest, massiteabevahendite kättesaadavusest, kinnipidamisasutuse välisest suhtlemisest, ettepanekutest ebavõrdse kohtlemise kõrvaldamiseks

  6. Thiol-ene Monolithic Pepsin Microreactor with a 3D-Printed Interface for Efficient UPLC-MS Peptide Mapping Analyses

    DEFF Research Database (Denmark)

    Jönsson, Alexander; Svejdal, Rasmus R; Bøgelund, Nanna

    2017-01-01

    To improve the sample handling, and reduce cost and preparation time, of peptide mapping LC-MS workflows in protein analytical research, we here investigate the possibility of replacing conventional enzymatic digestion methods with a polymer microfluidic chip based enzyme reactor. Off-stoichiomet...... used in LC-MS based protein analyses. The chip IMER is found to rival the conventional column in terms of digestion efficiency at comparable residence time and, using a 3D-printed interface, be directly interfaceable with LC-MS....

  7. (1RS,4RS,5RS-Methyl 2-(3,5-dinitrobenzoyl-2-oxa-3-azabicyclo[3.3.0]oct-7-ene-4-carboxylate

    Directory of Open Access Journals (Sweden)

    Xerardo Garcia-Mera

    2009-05-01

    Full Text Available The title compound, C15H13N3O8, comprises two crystallographically independent molecules in the asymmetric unit. In the crystal, intermolecular C—H...O hydrogen bonds link the molecules and short C=O...π contacts are seen.

  8. Riigi ülesannete täitmine lennuliiklusteeninduse korraldamisel : [bakalaureusetöö] / Imre Ernits ; Tartu Ülikool, õigusteaduskond ; juhendaja: Ene Andresen

    Index Scriptorium Estoniae

    Ernits, Imre

    2010-01-01

    Lennuohutuse tagamise funktsioonidest ja sellest tulenevatest riiklikest ülesannetest, Euroopa Liidu ja rahvusvahelisest õigusest tulenevatest nõuetest lennuliiklusteeninduse korraldamisele. Lennuliiklusteeninduse korraldusest Eesti õiguses, põhiseaduslikest probleemidest

  9. Zašto svi želimo biti lijepi i mladi (a žene naročito)? iz perspektive evolucijske psihologije

    OpenAIRE

    Tadinac, Meri

    2010-01-01

    Prema postavkama standardnog modela društevenih znanosti standardi ljepote su kulturalno određeni i usvajaju se tijekom socijalizacije. Međutim, dvije su skupine nalaza dovele u pitanje ovu uobičajenu pretpostavku: prvo, pokazalo se da se standardi ljepote javljaju vrlo rano u životu, što sugerira da bi mogli biti urođeni, te drugo, pokazalo se da su standardi ljepote međukulturalno univerzalni. Ovakvi nalazi potkrepljuju stajalište evolucijske psihologije prema kojem ljepota nije kulturalni ...

  10. Pakkujate võrdne kohtlemine riigihankemenetlusest kõrvaldamisel ja pakkujate kvalifitseerimisel : [magistritöö] / Piia Kulm ; Tartu Ülikool, õigusteaduskond ; juhendajad: Ene Andresen, Veiko Vaske

    Index Scriptorium Estoniae

    Kulm, Piia

    2009-01-01

    Võrdse kohtlemise põhimõtte sisust ja kohaldamisest ning selle põhimõtte järgimise tagamisest riigihangete õiguses, pakkujate kõrvaldamise ja pakkujate kvalifitseerimise etapi erinevustest, pakkujate hankemenetlusest kõrvaldamise ning kvalifitseerimise etapist

  11. Dimeric cyanobacterial cyclopent-4-ene-1,3-dione as selective inhibitor of Gram-positive bacteria growth: Bio-production approach and preparative isolation by HPCCC

    Czech Academy of Sciences Publication Activity Database

    Cheel, José; Bogdanová, K.; Ignatova, S.; Garrard, I.; Hewitson, P.; Kolář, M.; Kopecký, Jiří; Hrouzek, Pavel; Vacek, J.

    2016-01-01

    Roč. 18, SEP (2016), s. 244-249 ISSN 2211-9264 R&D Projects: GA MŠk EE2.3.30.0059; GA MŠk ED2.1.00/03.0110; GA MŠk(CZ) LO1416 Institutional support: RVO:61388971 Keywords : Phenolic cyclopentenedione * Natural antibiotic * Cyanobacteria Subject RIV: EE - Microbiology, Virology Impact factor: 3.994, year: 2016

  12. Process for Biotransformation of Androsta-4-ene-3, 17-Dione (4-AD) to Androsta-1,4-Diene-3,17-Dione (ADD).

    Science.gov (United States)

    Prakash, Surya; Bajaj, Abhay

    2017-01-01

    Androsta-1,4-diene-3,17-dione (androstadienedione, ADD) is key intermediate for the organic synthesis of a variety of female sex hormones such as estrone, estradiol, estriol and other related derivatives. De novo synthesis of this molecule is not yet reported in any form of living system, i.e., microbial, plant, and animal. The structural complexities due to presence of several chiral carbon centers create significant hurdles in chemical synthesis of such molecules. Microbe-mediated biotransformation offer a highly reliable, cost-effective, and relatively non hazardous way for commercial manufacturing of steroidal key intermediates. Currently microbial biotransformations are extensively being exploited for large-scale production of basic intermediates such as androstenedione (AD), ADD, and several types of hydroxylated derivatives of androstane compounds. In this chapter several aspects of microbial biotransformation process of AD to ADD are discussed.

  13. A Study on the Electro-Optical Properties of Thiol-Ene Polymer Dispersed Cholesteric Liquid Crystal (PDChLC) Films.

    Science.gov (United States)

    Sun, Yujian; Gao, Yanzi; Zhou, Le; Huang, Jianhua; Fang, Hua; Ma, Haipeng; Zhang, Yi; Yang, Jie; Song, Ping; Zhang, Cuihong; Zhang, Lanying; Li, Fasheng; Zhao, Yuzhen; Li, Kexuan

    2017-02-22

    In this study, a polymer dispersed cholesteric liquid crystal (PDChLC) film obtained via a one-step fabrication technique based on photopolymerization of a thiol-acrylate reaction system was prepared and characterized for the first time. The effects of the chiral dopant, the influence of thiol monomer functionality and content on the morphology and subsequent performance of the PDChLC films were systematically investigated. It was demonstrated that the addition of a small amount of chiral dopant slightly increased the driving voltage, but decreased the off-state transmittance significantly. Furthermore, scanning electron micrographs (SEM) shown that the liquid crystal (LC) droplet size decreased at first and then increased with the increasing amount of thiol monomer functionality, while increasing the thiol content increased the LC droplet size. Correspondingly, the electro-optical switching behavior was directly dependent on LC droplet size. By tuning the raw material composition, PDChLC film with optimized electro-optical performance was prepared.

  14. A Study on the Electro-Optical Properties of Thiol-Ene Polymer Dispersed Cholesteric Liquid Crystal (PDChLC Films

    Directory of Open Access Journals (Sweden)

    Yujian Sun

    2017-02-01

    Full Text Available In this study, a polymer dispersed cholesteric liquid crystal (PDChLC film obtained via a one-step fabrication technique based on photopolymerization of a thiol-acrylate reaction system was prepared and characterized for the first time. The effects of the chiral dopant, the influence of thiol monomer functionality and content on the morphology and subsequent performance of the PDChLC films were systematically investigated. It was demonstrated that the addition of a small amount of chiral dopant slightly increased the driving voltage, but decreased the off-state transmittance significantly. Furthermore, scanning electron micrographs (SEM shown that the liquid crystal (LC droplet size decreased at first and then increased with the increasing amount of thiol monomer functionality, while increasing the thiol content increased the LC droplet size. Correspondingly, the electro-optical switching behavior was directly dependent on LC droplet size. By tuning the raw material composition, PDChLC film with optimized electro-optical performance was prepared.

  15. Tavakoolide õpetajate suhtumine kõigi laste koosõpetamisse ja nende oskused erivajadustega laste õpetamisel tavaklassis / Ene Mägi, Aina Haljaste

    Index Scriptorium Estoniae

    Mägi, Ene, 1939-

    2001-01-01

    1998/99. õa. Eesti tavakoolides töötavate õpetajate seas läbi viidud küsitlusest: milline on õpetajate suhtumine kõigi laste koosõpetamisse ning missugused on õpetajate oskused ja vajadused erivajadustega laste õpetamisel tavaklassis

  16. 1α,6β,7β,11α,15β-Pentahydroxy-7α,20-epoxy-ent-kaur-16-ene

    Directory of Open Access Journals (Sweden)

    Chuang Feng

    2010-02-01

    Full Text Available The title compound, C20H30O6, a natural ent-kaurane diterpenoid, named nervosanin B, was obtained from the medicinal plant Isodon serra. It is composed of four rings with the expected trans and cis junctions. One of the six-membered rings is in a chair conformation, the other two are in boat conformations and the five-membered ring adopts an evenlope conformation. The molecules stack along the a axis and are linked together by intermolecular O—H...O hydrogen bonds. Two intramolecular O—H...O interactions also occur.

  17. 6β-Acetoxy-1α,7β,11β,15β-tetrahydroxy-7α,20-epoxy-ent-kaur-16-ene

    Directory of Open Access Journals (Sweden)

    Fu-Lin Yan

    2010-02-01

    Full Text Available The title compound, C22H32O7, a natural ent-kaurane diterpenoid also referred to as Maoyecrystal F, was obtained from the medicinal plant Isodon nervosa. There are four rings with the expected cis and trans junctions. Cyclohexane ring A adopts a chair conformation, rings B and C adopt boat conformations, while the five-membered ring has an envelope conformation. The molecules stack along the a axis in the crystal and are linked together by intermolecular O—H...O hydrogen bonds.

  18. 15α,20β-Dihydroxy-6β-methoxy-6,7-seco-6,20-epoxy-1,7-olide-ent-kaur-16-ene

    Directory of Open Access Journals (Sweden)

    Fu-Lin Yan

    2010-04-01

    Full Text Available The title compound, C21H30O6, a natural ent-kaurane diterpenoid, was obtained from the medicinal plant Isodon serra. The five rings in the molecule exhibit the expected cis and trans junctions. The three six-membered rings adopt chair, twist-boat and boat conformations, while two five-membered rings adopt envelope conformations. There are two molecules in the asymmetric unit, related by a non-crystallographic twofold screw axis; the main difference is in the different degrees of distortion of ring B. In the crystal, the molecules are linked by intermolecular O—H...O hydrogen bonds, forming chains along the b axis.

  19. (1R,2R,3R,4S,5S-3-Methyl-8-oxabicyclo[3.2.1]oct-6-ene-2,4-diyl diacetate

    Directory of Open Access Journals (Sweden)

    Viktor A. Tafeenko

    2011-08-01

    Full Text Available The molecule of the title compound, C12H16O5, has crystallographically imposed mirror symmetry with the mirror plane passing through the endocyclic O atom and the mid-point of the double bond. In the crystal, molecules are linked by C—H...O hydrogen bonds, forming chains running along the a axis.

  20. Mass spectrometric analysis of oxygenations in aromatization of androst-4-ene-3,6,17-trione, a suicide substrate of aromatase, by placental microsomes. Isotope effect and stereochemistry.

    Science.gov (United States)

    Numazawa, Mitsuteru; Takahashi, Madoka; Nagaoka, Masao; Handa, Wakako; Yamashita, Kouwa

    2007-01-01

    Aromatase catalyzes the conversion of androstenedione (AD) to estrone through three sequential oxygenations of the 19-methyl group. 6-OxoAD (1) is one of the typical suicide substrates of aromatase, which is converted by aromatase to 6-oxoestrone through 19-alcohol (19-ol) and 19-aldehyde (19-al) intermediates 2 and 3. To study the deuterium isotope effect on the conversion of 19-ol 2 to 19-al 3 as well as the stereochemistry of the 19-hydrogen removal in this conversion, we initially synthesized [19,19-(2)H(2)] and [19S- or 19R-(2)H] 19-ols 2, starting from the corresponding deuterium-labeled 19-hydroxyAD derivatives. In incubation of non-labeled and [19,19-(2)H(2)]-labeled 19-ol 2 or that of their 1:1 mixture with human placental microsomes in the presence of NADPH under air, there was no significant deuterium-isotope effect on the production of the aromatized product 6-oxoestrone or on the conversion of 19-ol 2 to 19-al 3, based on gas chromatography-mass spectrometric analysis of the estrogen product or liquid chromatography-mass spectrometric (LC-MS) analysis of the deuterium contents of the product 19-al 3 and the recovered 19-ol 2. Moreover, in the incubations of [19S-(2)H] 19-ol 2 and its 19R isomer, LC-MS analysis of the product 3 demonstrated that the 19-pro-R hydrogen atom was stereospecifically removed in the conversion of 19-ol 2 to 19-al 3. These findings indicate that the 19-oxygenation of 19-ol 2 would proceed in the same mechanism as that involved in the AD aromatization.