WorldWideScience

Sample records for vol 3b volume

  1. Vol(2)velle: Printable Interactive Volume Visualization.

    Science.gov (United States)

    Stoppel, Sergej; Bruckner, Stefan

    2017-01-01

    Interaction is an indispensable aspect of data visualization. The presentation of volumetric data, in particular, often significantly benefits from interactive manipulation of parameters such as transfer functions, rendering styles, or clipping planes. However, when we want to create hardcopies of such visualizations, this essential aspect is lost. In this paper, we present a novel approach for creating hardcopies of volume visualizations which preserves a certain degree of interactivity. We present a method for automatically generating Volvelles, printable tangible wheel charts that can be manipulated to explore different parameter settings. Our interactive system allows the flexible mapping of arbitrary visualization parameters and supports advanced features such as linked views. The resulting designs can be easily reproduced using a standard printer and assembled within a few minutes.

  2. A Review of Soviet Studies in the Psychology of Learning and Teaching Mathematics (6 Volumes). Proceedings of the National Academy of Education, Vol. 4, 1977.

    Science.gov (United States)

    van Bruggen, Johan C.; Freudenthal, Hans

    The English translations of the first six volumes of Studies on the Teaching and Learning of Mathematics in the Soviet Union are viewed in this document. The contents of each volume are briefly described: vol. 1, The Learning of Mathematical Concepts; vol. 2, The Structure of Mathematical Abilities; vol. 3, Problem Solving in Arithmetic and…

  3. Waste Receiving and Processing Facility Module 2A: Advanced Conceptual Design Report. Volume 3B

    Energy Technology Data Exchange (ETDEWEB)

    1994-03-01

    This volume consists of the following sections: WRAP 2A value engineering assessment, resolution of value engineering assessment actions (white paper), HAZOP studies for identifying major safety and operability problems, and time and motion simulation.

  4. Automatic individualized contrast medium dosage during hepatic computed tomography by using computed tomography dose index volume (CTDI{sub vol})

    Energy Technology Data Exchange (ETDEWEB)

    Svensson, Anders; Cederlund, Kerstin; Aspelin, Peter; Brismar, Torkel B. [Intervention and Technology at Karolinska Institutet, Department of Clinical Science, Division of Medical Imaging and Technology, Stockholm (Sweden); Karolinska University Hospital in Huddinge, Department of Radiology, Stockholm (Sweden); Bjoerk, Jonas [FoU-centrum Skaane Skaanes Universitetssjukhus i Lund, Lund (Sweden); Nyman, Ulf [University of Lund, Department of Diagnostic Radiology, Lasarettet Trelleborg, Trelleborg (Sweden)

    2014-08-15

    To compare hepatic parenchymal contrast media (CM) enhancement during multi-detector row computed tomography (MDCT) and its correlation with volume pitch-corrected computed tomography dose index (CTDI{sub vol}) and body weight (BW). One hundred patients referred for standard three-phase thoraco-abdominal MDCT examination were enrolled. BW was measured in the CT suite. Forty grams of iodine was administered intravenously (iodixanol 320 mg I/ml at 5 ml/s or iomeprol 400 mg I/ml at 4 ml/s) followed by a 50-ml saline flush. CTDI{sub vol} presented by the CT equipment during the parenchymal examination was recorded. The CM enhancement of the liver was defined as the attenuation HU of the liver parenchyma during the hepatic parenchymal phase minus the attenuation in the native phase. Liver parenchymal enhancement was negatively correlated to both CTDI{sub vol} (r = -0.60) and BW (r = -0.64), but the difference in correlation between those two was not significant. CTDI{sub vol} may replace BW when adjusting CM doses to body size. This makes it potentially feasible to automatically individualize CM dosage by CT. (orig.)

  5. Compendium of NASA Data Base for the Global Tropospheric Experiment's Transport and Chemical Evolution Over the Pacific (TRACE-P). Volume 2; P-3B

    Science.gov (United States)

    Kleb, Mary M.; Scott, A. Donald, Jr.

    2003-01-01

    This report provides a compendium of NASA aircraft data that are available from NASA's Global Tropospheric Experiment's (GTE) Transport and Chemical Evolution over the Pacific (TRACE-P) Mission. The broad goal of TRACE-P was to characterize the transit and evolution of the Asian outflow over the western Pacific. Conducted from February 24 through April 10, 2001, TRACE-P integrated airborne, satellite- and ground based observations, as well as forecasts from aerosol and chemistry models. The format of this compendium utilizes data plots (time series) of selected data acquired aboard the NASA/Dryden DC-8 (vol. 1) and NASA/Wallops P-3B (vol. 2) aircraft during TRACE-P. The purpose of this document is to provide a representation of aircraft data that are available in archived format via NASA Langley's Distributed Active Archive Center (DAAC) and through the GTE Project Office archive. The data format is not intended to support original research/analyses, but to assist the reader in identifying data that are of interest.

  6. International Source Book: Nuclear Fuel Cycle Research and Development Vol 1 Volume 1

    Energy Technology Data Exchange (ETDEWEB)

    Harmon, K. M. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Lakey, L. T. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    1983-07-01

    This document starts with an overview that summarizes nuclear power policies and waste management activities for nations with significant commercial nuclear fuel cycle activities either under way or planned. A more detailed program summary is then included for each country or international agency conducting nuclear fuel cycle and waste management research and development. This first volume includes the overview and the program summaries of those countries listed alphabetically from Argentina to Italy.

  7. AGARD Flight Test Series. Volume 10. Weapon Delivery Analysis and Ballistic Flight Testing (L’Analyse du Largage d’Armes et les en Vol Balistique).

    Science.gov (United States)

    1992-07-01

    de ce volume figure ci-dessous. LAGARD peut etre tier que ces personnes competentes aient bien voulu accepter de partager leurs connaissances et...de travail sur les techniques des essais en vol a ete recree pour mener ä bien cette täche. Les monographies dans cette serie (a l’exception de la AG...DOIVENT ETRE ADRESSEES DIRECTEMENT AU SERVICE NATIONAL TECHNIQUE DE ^INFORMATION (NTIS) DONT LADRESSE SUIT. National Technical Information Service

  8. Image quality and volume computed tomography air kerma index (C{sub vol}) evaluation in Recife; Avaliacao da qualidade de imagem e do indice volumetrico de Kerma ar em tomografia computadorizada (C{sub vol}) em Recife

    Energy Technology Data Exchange (ETDEWEB)

    Andrade, Marcos Ely Almeida

    2008-07-01

    The Computed Tomography (CT) is an important diagnostic imaging method, widely used. However, in spite of all the advantages and technologic advances within the CT scanners, the tomographic procedures result in high absorbed doses to patients. The main objective of this work was to perform a dosimetric study of CT scanners located at Recife and to evaluate the image quality on CT examinations in these equipment. The volume CT air kerma index (C{sub VOL}) and air kerma length product (P{sub KL,CT}) were estimated. These values were calculated using normalized weighted air kerma indexes in CT standard dosimetry phantoms ({sub n}C{sub W}), supplied by ImPACT group for several CT scanners, and the scan parameters of routine head, routine chest and hi-resolution chest CT exams performed at 20 institutions. The irradiation parameters of 15 adult patients for each CT procedure were registered at six participating centres, at which the phantom from the American College of Radiology (ACR) CT accreditation protocol was used for the image quality measurements. For routine head exams, the C{sub VOL} values varied between 12 and 58 mGy (at the posterior fossa) and 15 to 58 mGy (at the cerebrum) and the P{sub KL,CT}, from 150 to 750 mGy{center_dot}cm. The C{sub VOL} values for routine chest procedures varied from 3 to 26 mGy and the P{sub KL,CT}, between 120 and 460 mGy{center_dot}cm. In relation to Hi-resolution chest exams, C{sub VOL} values were from 1.0 to 2.7 mGy and the P{sub KL,CT} values varied between 24 and 67 mGy{center_dot}cm. The image quality evaluations results showed that almost all scanners presented at least one inadequacy. One of the equipment presented faults at 70% of the tests. With regard to the image noise, only two scanners presented acceptable results. From these results, it is possible to conclude that the volume CT air kerma index values are lower than the European reference levels. However, the image quality of these CT scanners does not attend the

  9. Effects of CO2 pneumoperitoneum on blood flow vol-ume of abdominal organs of rabbits with controlled hem-orrhagic shock and liver impact injuries

    Institute of Scientific and Technical Information of China (English)

    ZHANG Lian-yang; ZHAO Song; LI Yong; MA Xiao-lin

    2009-01-01

    Objective: To investigate the effects of CO2 pneumo-peritoneum on blood flow volume of abdominal organs of rabbits with controlled hemorrhagic shock model and liver impact injuries.Methods: After controlled hemorrhagic shock and liver impact injuries, the rabbit model was established. Eighteen rabbits subjected to hemorrhagic shock and liver impact inju-ries were divided into 3 groups randomly according to the volume of lost blood: light hemorrhagic shock (blood loss volume was 10%, 6 ml/kg), moderate hemorrhagic shock (20%, 12 ml/kg) and severe hemorrhagic shock (40%, 22 ml/kg). Intraabdominal pressures of CO2 pneumoperitoneum was 10 mmHg. Color-labeled microspheres were used to mea-sure the blood flow volume of the liver, kidney and stomach before pneumoperitoneum at 30 minutes and 2 hours after pneumoperitoneum and 30 minutes after deflation. And the mortality and hepatic traumatic condition of rabbits were recorded.Results: Of the 18 rabbits, there were 9 with liver impact injuries at Grade Ⅰ, 8 at Grade Ⅱ and Ⅰ at Grade Ⅲ (according to AIS-2005). The mortality rate in light hemorrhagic shock group was 33.33%, and that in moderate or severe hemor-rhagic shock group was 100% within 30 minutes and 2 hours after pneumoperitoneum, respectively. The blood flow vol-ume in the organs detected decreased at 30 minutes under pneumoperitoneum in light and moderate hemorrhagic shock groups. At the same time, the blood flow volume of the liver in moderate hemorrhagic shock group decreased more sig-nificantly than that in light hemorrhagic shock group.Conclusions: The blood flow volume of abdominal organs in rabbits is decreased obviously under CO2 pneumoperitoneum, with fairly high mortality rate. It is be-lieved that CO2 pneumoperitoneum should cautiously be used in abdominal injury accompanied with hemorrhagic shock, especially under non-resuscitation conditions.

  10. Volúmenes pulmonares normales en pacientes con fibrosis pulmonar idiopática y enfisema Normal lung volumes in patients with idiopathic pulmonary fibrosis and emphysema

    Directory of Open Access Journals (Sweden)

    Juan Pablo Casas

    2008-08-01

    Full Text Available La fibrosis pulmonar idiopática (FPI es una enfermedad que se caracteriza por presentar un compromiso pulmonar de tipo restrictivo, resultante de una reducción en la complacencia pulmonar secundaria a fibrosis difusa. En el enfisema, la pérdida de elasticidad pulmonar y el colapso de las vías aéreas periféricas generan obstrucción e hiperinflación. El efecto simultáneo que ambas enfermedades producen sobre la fisiología pulmonar no es del todo claro y se han descripto volúmenes pulmonares normales o casi normales. Presentamos 4 pacientes de sexo masculino de 64, 60, 73 y 70 años, con antecedentes de tabaquismo e historia de disnea progresiva, tres de ellos con grave limitación en su calidad de vida al momento de la consulta. En la tomografía de tórax de alta resolución todos los pacientes presentaban signos de enfermedad intersticial pulmonar avanzada, con cambios de tipo fibrótico con predominio basal y subpleural, que coexistían con enfisema centroacinar con predominio en lóbulos superiores. Uno de ellos tuvo confirmación diagnóstica de ambas condicioes por biopsia pulmonar a cielo abierto. En los cuatro pacientes la espirometría y volúmenes pulmonares fueron normales, pero tenían importante compromiso del intercambio gaseoso evaluado mediante el test de caminata de 6 minutos. Tres de los pacientes tenían hipertensión pulmonar grave diagnosticado por ecocardiograma. La presencia de volúmenes pulmonares normales no excluye un diagnóstico de fibrosis pulmonar idiopática en pacientes fumadores si coexisten evidencias tomográficas de enfisema. En estos pacientes el grado de compromiso funcional, determinado por la reducción de los volúmenes pulmonares, no debería ser considerado en la evaluación de la gravedad.Pulmonary function tests in idiopathic pulmonary fibrosis characteristically show a restrictive pattern, resulting from reduction of pulmonary compliance due to diffuse fibrosis. Conversely, an obstructive

  11. Oak Ridge K-25 Site Technology Logic Diagram. Volume 3, Technology evaluation data sheets; Part B, Remedial action, robotics/automation, waste management

    Energy Technology Data Exchange (ETDEWEB)

    Fellows, R.L. [ed.

    1993-02-26

    The Oak Ridge K-25 Technology Logic Diagram (TLD), a decision support tool for the K-25 Site, was developed to provide a planning document that relates environmental restoration (ER) and waste management (WN) problems at the Oak Ridge K-25 Site. The TLD technique identifies the research necessary to develop these technologies to a state that allows for technology transfer and application to waste management, remediation, decontamination, and decommissioning activities. The TLD consists of four separate volumes-Vol. 1, Vol. 2, Vol. 3A, and Vol. 3B. Volume 1 provides introductory and overview information about the TLD. Volume 2 contains logic diagrams. Volume 3 has been divided into two separate volumes to facilitate handling and use. This volume 3 B provides the Technology Evaluation Data Sheets (TEDS) for ER/WM activities (Remedial Action Robotics and Automation, Waste Management) that are referenced by a TEDS code number in Vol. 2 of the TLD. Each of these sheets represents a single logic trace across the TLD. These sheets contain more detail than each technology in Vol. 2. The TEDS are arranged alphanumerically by the TEDS code number in the upper right corner of each data sheet. Volume 3 can be used in two ways: (1) technologies that are identified from Vol. 2 can be referenced directly in Vol. 3 by using the TEDS codes, and (2) technologies and general technology areas (alternatives) can be located in the index in the front of this volume.

  12. Ecological evaluation of proposed discharge of dredged material from Oakland Harbor into ocean waters (Phase 3 B of -42-foot project). Volume 1, Analyses and discussion

    Energy Technology Data Exchange (ETDEWEB)

    Kohn, N.P.; Ward, J.A.; Mayhew, H.L.; Word, J.Q.; Barrows, E.S.; Goodwin, S.M.; Lefkovitz, L.F. [Battelle/Marine Sciences Lab., Sequim, WA (United States)

    1992-06-01

    The Water Resources Development Act of 1986 (Public Law 99-662) authorized the US Army Corps of Engineers (USACE) San Francisco District, to deepen and widen the navigational channels of the Oakland Inner and Outer Harbors to accomodate deeper-draft vessels. The USACE is considering several disposal options for the dredged material removed during these channel improvements including open-water disposal. Dredged material proposed for open-water disposal must be evaluated to determine the potential impacts of the disposal activity on the water column and disposal site enviromments. The USACE requested that Battelle/Marine Sciences Laboratory (MSL) conduct studies to evaluate open-water disposal options for Oakland Harbor sediments. This request developed into the Oakland Harbor Phase III Program. This is Volume 1 of a two-volume report that presents information gathered to determine the suitability of ocean disposal of sediments dredged from Oakland Harbor. This volume contains project background, materials and methods, results, discussion, and conclusions.

  13. Data catalog series for space science and applications flight missions. Volume 3B: Descriptions of data sets from low- and medium-altitude scientific spacecraft and investigations

    Science.gov (United States)

    Jackson, John E. (Editor); Horowitz, Richard (Editor)

    1986-01-01

    The main purpose of the data catalog series is to provide descriptive references to data generated by space science flight missions. The data sets described include all of the actual holdings of the Space Science Data Center (NSSDC), all data sets for which direct contact information is available, and some data collections held and serviced by foreign investigators, NASA and other U.S. government agencies. This volume contains narrative descriptions of data sets from low and medium altitude scientific spacecraft and investigations. The following spacecraft series are included: Mariner, Pioneer, Pioneer Venus, Venera, Viking, Voyager, and Helios. Separate indexes to the planetary and interplanetary missions are also provided.

  14. Oak Ridge K-25 Site Technology Logic Diagram. Volume 2, Technology Logic Diagrams

    Energy Technology Data Exchange (ETDEWEB)

    Fellows, R.L. [ed.

    1993-02-26

    The Oak Ridge K-25 Technology Logic Diagram (TLD), a decision support tool for the K-25 Site, was developed to provide a planning document that relates envirorunental restoration and waste management problems at the Oak Ridge K-25 Site to potential technologies that can remediate these problems. The TLD technique identifies the research necessary to develop these technologies to a state that allows for technology transfer and application to waste management, remedial action, and decontamination and decommissioning activities. The TLD consists of four separate volumes-Vol. 1, Vol. 2, Vol. 3A, and Vol. 3B. Volume 1 provides introductory and overview information about the TLD. This volume, Volume 2, contains logic diagrams with an index. Volume 3 has been divided into two separate volumes to facilitate handling and use.

  15. Flight Test Measurement Techniques for Laminar Flow. Volume 23(Les techniques de mesure en vol des ecoulements laminaires)

    Science.gov (United States)

    2003-10-01

    aspects of Volume 1 and 2 of the original Flight Test Manual , including the flight testing of aircraft systems. The monographs in this series (with the...N. Alemdaroglu, Middle East Technical University, Ankara, Turkey, Prof. L.M.B. da Costa Campos, Instituto Superior Tecnico , Lisboa Codex, Portugal...Defence Research Establishment ITALIE Česká republika Ryvangs Allé 1, P.O. Box 2715 Centro di Documentazione DK-2100 Copenhagen Ø Tecnico

  16. Oak Ridge K-25 Site Technology Logic Diagram. Volume 3, Technology evaluation data sheets; Part A, Characterization, decontamination, dismantlement

    Energy Technology Data Exchange (ETDEWEB)

    Fellows, R.L. [ed.

    1993-02-26

    The Oak Ridge K-25 Technology Logic Diagram (TLD), a decision support tool for the K-25 Site, was developed to provide a planning document that relates environmental restoration and waste management problems at the Oak Ridge K-25 Site to potential technologies that can remediate these problems. The TLD technique identifies the research necessary to develop these technologies to a state that allows for technology transfer and application to waste management, remedial action, and decontamination and decommissioning activities. The TLD consists of four separate volumes-Vol. 1, Vol. 2, Vol. 3A, and Vol. 3B. Volume 1 provides introductory and overview information about the TLD. Volume 2 contains logic diagrams. Volume 3 has been divided into two separate volumes to facilitate handling and use. This report is part A of Volume 3 concerning characterization, decontamination, and dismantlement.

  17. Index to the Journal of American Indian Education, Vol. 1, No. 1 - Vol. 8, No. 1.

    Science.gov (United States)

    Loomis, Charlotte Ann

    All articles (112) that appeared in the "Journal of American Indian Education" (JAIE), Vol. 1., No. 1 (June 1961) through Vol. 8, No 1 (October 1968) are indexed and annotated. The publication is divided into 3 parts: (1) annotations listed in order of appearance in JAIE by volume, number, and page; (2) author index; and (3) subject index. Later…

  18. Proceedings of the International Congress (12th), Corrosion Control for Low-Cost Reliability, Held in Houston, Texas on September 19 -24, 1993. Volume 3B. Corrosion: Specific Issues

    Science.gov (United States)

    1993-09-24

    de Quimica Pura y Aplicada, Monterrey N.L., 1990. No. 47. 3.- 8. Valdez, G. Guerra, J. Sampedro, N. Rosas. CORROSION, Vol. 2, No. 3, 1991, pp. 11-15...test was carried out at ambient temperature for 72 hours and wheel speed was kept 6 r.p.m. Table no. 1 shows the experimental results in percentage...described in earlier work (3) which predicted the performance of stainless steels in simple chloride containing waters at ambient temperature. The model

  19. Volúmenes pulmonares normales en pacientes con fibrosis pulmonar idiopática y enfisema Normal lung volumes in patients with idiopathic pulmonary fibrosis and emphysema

    OpenAIRE

    Juan Pablo Casas; Horacio Abbona; Adriana Robles; Ana María López

    2008-01-01

    La fibrosis pulmonar idiopática (FPI) es una enfermedad que se caracteriza por presentar un compromiso pulmonar de tipo restrictivo, resultante de una reducción en la complacencia pulmonar secundaria a fibrosis difusa. En el enfisema, la pérdida de elasticidad pulmonar y el colapso de las vías aéreas periféricas generan obstrucción e hiperinflación. El efecto simultáneo que ambas enfermedades producen sobre la fisiología pulmonar no es del todo claro y se han descripto volúmenes pulmonares no...

  20. Dual-energy X-ray absorptiometry and forced expiratory volumes in sedentary and trained children. [Absorciometria dual de rayos x y volúmenes espirométricos forzados en niños sedentarios y entrenados].

    Directory of Open Access Journals (Sweden)

    Álvaro Fernández-Luna

    2015-10-01

    Full Text Available Information regarding the influence of physical activity and fat mass in the human trunk on respiratory health is scarce. The aim of this study was to evaluate the influence of body composition and physical activity in forced expiratory volumes in children. The sample comprised 87 children between 8 and 13 years of age (9.62 ± 1.25, at Tanner stages I-III, participated in this study. Sixty played sports (trained children, TC and 27 were assigned to the sedentary children control group (SC. Body composition (DXA, anthropometric variables (weight and height, and forced expiratory volumes (FEV1 and FVC were determined in all subjects. Results showed that higher FEV1 and FVC values were found in the SC group compared to the TC group. Multiple regression analysis showed that physical activity, total fat mass, and android fat mass, had the highest predictive value for FVE1 as did height for the FVC. In SC group there was a positive association between FEV1/FVC ratio and Body Mass Index and trunk fat mass. We did not find any associations between forced respiratory volumes and the whole body and trunk region fat mass in trained and sedentary children. Resumen La información sobre los diferentes efectos de la actividad física y la masa grasa del tronco en la salud respiratoria es escasa. El objetivo fue evaluar la influencia de las variables de composición corporal y la práctica de actividad física en los volúmenes espirométricos forzados en edad infantil. 87 niños entre los 8 y 13 años (9,62 ± 1,25 (Tanner I-III participaron en el estudio. 60 practicaban diferentes deportes (niños entrenados, TC y 27 niños sedentarios fueron asignados al grupo control (SC. Se llevó a cabo la evaluación en todos los participantes de la composición corporal a través de DEXA, variables antropométricas (peso y altura y volúmenes espirométricos forzados (FEV1 and FVC. Los resultados mostraron que el grupo SC tenía unos volúmenes FEV1 y FVC superiores

  1. LM-3B Launch Vehicle

    Institute of Scientific and Technical Information of China (English)

    RenShufang

    2005-01-01

    LM-3B launch vehicle is a heavy three-stage liquid propellant strap-on launch vehicle, which was developed based on the mature technologies of the LM-3A and LM-2E. It not only has the highest payload capacity to send China's satellites to GTO, but is also one of the most advanced launch vehicles in the world with high reliability, reasonable price and perfect technological design.

  2. The collected papers of Albert Einstein. - Vol. 11: Cumulative index, bibliography, list of correspondence, chronology, and errata to volumes 1-10

    NARCIS (Netherlands)

    Kox, A.J.; Sauer, T.; Kormos Buchwald, D.; Hirschmann, R.; Moses, O.; Aronin, B.; Stolper, J.

    2009-01-01

    This index volume provides quick access to the most authoritative compilation of documents and information concerning Einstein's work and correspondence for the first half of his life. It offers readers a Cumulative Index to the first ten volumes of the collected papers, the first complete bibliogra

  3. Evaluation of potential severe accidents during low power and shutdown operations at Surry, Unit 1: Analysis of core damage frequency from internal events during mid-loop operations. Appendix E (Sections E.9-E.16), Volume 2, Part 3B

    Energy Technology Data Exchange (ETDEWEB)

    Chu, T.L.; Musicki, Z.; Kohut, P.; Yang, J.; Bozoki, G.; Hsu, C.J.; Diamond, D.J.; Wong, S.M. [Brookhaven National Lab., Upton, NY (United States); Bley, D.; Johnson, D. [PLG Inc., Newport Beach, CA (United States)] [and others

    1994-06-01

    Traditionally, probabilistic risk assessments (PRA) of severe accidents in nuclear power plants have considered initiating events potentially occurring only during full power operation. Some previous screening analyses that were performed for other modes of operation suggested that risks during those modes were small relative to full power operation. However, more recent studies and operational experience have implied that accidents during low power and shutdown could be significant contributors to risk. Two plants, Surry (pressurized water reactor) and Grand Gulf (boiling water reactor), were selected as the plants to be studied. The objectives of the program are to assess the risks of severe accidents initiated during plant operational states other than full power operation and to compare the estimated core damage frequencies, important accident sequences and other qualitative and quantitative results with those accidents initiated during full power operation as assessed in NUREG-1150. The scope of the program includes that of a level-3 PRA. In phase 2, mid-loop operation was selected as the plant configuration to be analyzed based on the results of the phase 1 study. The objective of the phase 2 study is to perform a detailed analysis of the potential accident scenarios that may occur during mid-loop operation, and compare the results with those of NUREG-1150. The scope of the level-1 study includes plant damage state analysis, and uncertainty analysis. Volume 1 summarizes the results of the study. Internal events analysis is documented in Volume 2. It also contains an appendix that documents the part of the phase 1 study that has to do with POSs other than mid-loop operation. Internal fire and internal flood analyses are documented in Volumes 3 and 4. A separate study on seismic analysis, documented in Volume 5, was performed for the NRC by Future Resources Associates, Inc. Volume 6 documents the accident progression, source terms, and consequence analysis.

  4. Ectopic DNMT3B expression delays leukemogenesis.

    Science.gov (United States)

    Stanley, Robert F; Steidl, Ulrich

    2016-03-24

    In this issue of Blood, Schulze et al use a tetracycline-inducible Dnmt3b knock-in mouse model to investigate how DNMT3B-mediated DNA methylation affects leukemogenesis. Increased DNMT3B expression prolonged survival in retrovirally induced Myc-Bcl2– or MLL-AF9–driven leukemia, and acute myeloid leukemia (AML) patients with high expression of DNMT3B target genes showed inferior overall survival.

  5. Itinerant magnetism in CeRh3B2

    DEFF Research Database (Denmark)

    Eriksson, Olle; Johansson, Börje; Brooks, M. S. S.

    1989-01-01

    Spin-polarized energy-band calculations, including spin-orbit coupling in the band Hamiltonian, have been performed on CeRh3B2. Good agreement is obtained between theory and experiment concerning the magnetic moment. It is also found that the magnetic moment varies strongly with volume and from...

  6. Oak Ridge K-25 Site Technology Logic Diagram. Volume 1, Technology evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Fellows, R.L. [ed.

    1993-02-26

    The Oak Ridge K-25 Technology Logic Diagram (TLD), a decision support tool for the K-25 Site, was developed to provide a planning document that relates environmental restoration and waste management problems at the Oak Ridge K-25 Site to potential technologies that can remediate these problems. The TLD technique identifies the research necessary to develop these technologies to a state that allows for technology transfer and application to waste management, remedial action, and decontamination and decommissioning activities. The TLD consists of four separate volumes-Vol. 1, Vol. 2, Vol. 3A, and Vol. 3B. This Volume, Volume 1 provides introductory and overview information about the TLD. Volume 2 contains logic diagrams. Volume 3 has been divided into two separate volumes to facilitate handling and use. This volume is divided into ten chapters. The first chapter is a brief introduction, and the second chapter details the technical approach of the TLD. These categories are the work activities necessary for successful decontamination and decommissioning, waste management, and remedial action of the K-25 Site. The categories are characterization, decontamination, dismantlement, robotics and automation, remedial action, and waste management. Materials disposition is addressed in Chap. 9. The final chapter contains regulatory compliance information concerning waste management, remedial action, and decontamination and decommissioning.

  7. The Relationship of VOl Threshold, Volume and B/S on DISA Images%双核素图像VOI阈值、体积和靶本比间关系

    Institute of Scientific and Technical Information of China (English)

    宋烈晶; 司宏伟; 王明明; 李飞

    2011-01-01

    为探讨SPECT双核素图像VOI阈值、体积和靶本比间关系,利用圆柱形体模内置10个空心球,按照1:7、1:6和1:5的靶本比,同时和分别注入99mTcO4-和18F-FDG,采集DISA和SIDA图像.采用阈值法勾画每个球体的感兴趣体积,拟合最佳阈值计算公式.最佳阈值计算公式为Tm=d+c×Bm/(e+f×Vm)+b/Vm.利用公式由球体真实体积计算的绝大多数阈值均在真实阈值所在的1%区间内,不在区间内者也处于该区间的上限或下限.无论DISA还是SIDA图像,利用VOI阈值、体积和靶本比间关系,以及球体真实体积均能较好地计算体积3.3~30.8 ml空心球体的最佳阈值,其误差小于1%.%Objective: To explore the relationshif of VOI threshold,Volume and B/S on DISA phantom images.Methods :Ten hollow spheres were placed in cylinder phantom. According to the B/S of 1:7, 1: 5 and 1:4,99mTcO4- and 18F- FDG was filled into the container and spheres simultaneously and separately. Images were acquired by DISA and SIDA protocol. Volume of interest (VOI) for each sphere was analyzed by threshold method and to fit expression individually for validating of the relationship. Results :The equation for the estimation of optimal threshold was as following Tm = d + c × Bm/(e + f × Vm) + b/Vm. In majority of data, the calculated threshold was in the 1% interval that optimal thresholds were really in. Those who were not in were at the lower or upper intervals. Conclusions: Both DISA and SIDA images, based o the relationship of VOl threshold, Volume and B/S and real volume, this method could accurately caculate optimal threshold with an error less than 1% for spheres whose volumes ranged from 3.3 to 30.8 ml.

  8. Enregistreur de vol

    OpenAIRE

    Osen, Alexander; Bianchi, Christophe

    2012-01-01

    Ce travail consiste à développer un prototype d'enregistreur de vol spécifique au moto-planeur électrique, il doit donc pouvoir enregistrer la position du planeur et détecter si le système de propulsion est en marche

  9. Integrated mined-area reclamation and land use planning. Volume 3B. A case study of surface mining and reclamation planning: international minerals and chemical corporation, phosphate operations, Polk County, Florida. [Land reclamation and use planning for phosphate operations in Polk County, Florida

    Energy Technology Data Exchange (ETDEWEB)

    LaFevers, J R; Brown, L A; Fountain, R C

    1977-02-01

    The reports in this series are designed primarily to familiarize professional land use and resource planners with the range of possibilities and effective procedures for achieving integrated mining, reclamation, and land use planning. These reports are based on a research program which included an extensive literature review, the compilation and analysis of case study data, and close coordination and interaction with related government programs. In Volume 3, A Guide to Mined Area Reclamation Technology for Reclamation and Land Use Planners, the method used to reclaim land in each of several mineral industries are discussed in relation to the physical and cultural constraints that must be considered in planning a reclamation program. Much of the information for this document was obtained from case studies conducted in several mining districts. Volume 3B presents data from a case study of surface mining and reclamation planning, International Minerals and Chemical Corporation, Phosphate Operations, Polk County, Florida.

  10. Detector production for the R3B Si-tracker

    Science.gov (United States)

    Borri, M.; Lemmon, R.; Thornhill, J.; Bate, R.; Chartier, M.; Clague, N.; Herzberg, R.-D.; Labiche, M.; Lindsay, S.; Nolan, P.; Pearce, F.; Powell, W.; Wells, D.

    2016-11-01

    R3B is a fixed target experiment which will study reactions with relativistic radioactive beams at FAIR. Its Si-tracker will surround the target volume and it will detect light charged-particles like protons. The detector technology in use consists of double-sided silicon strip sensors wire bonded to the custom made R3B-ASIC. The tracker allows for a maximum of two outer layers and one inner layer. This paper reports on the production of detectors necessary to build the minimum tracking configuration: one inner layer and one outer layer.

  11. 18 CFR 3b.4 - Government contractors.

    Science.gov (United States)

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Government contractors. 3b.4 Section 3b.4 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES COLLECTION, MAINTENANCE, USE, AND DISSEMINATION OF RECORDS OF...

  12. 18 CFR 3b.1 - Purpose.

    Science.gov (United States)

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Purpose. 3b.1 Section 3b.1 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES COLLECTION, MAINTENANCE, USE, AND DISSEMINATION OF RECORDS OF IDENTIFIABLE...

  13. 18 CFR 3b.2 - Definitions.

    Science.gov (United States)

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Definitions. 3b.2 Section 3b.2 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES COLLECTION, MAINTENANCE, USE, AND DISSEMINATION OF RECORDS OF IDENTIFIABLE...

  14. 18 CFR 3b.3 - Notice requirements.

    Science.gov (United States)

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Notice requirements. 3b.3 Section 3b.3 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES COLLECTION, MAINTENANCE, USE, AND DISSEMINATION OF RECORDS OF...

  15. Itinerant magnetism in CeRh3B2

    OpenAIRE

    1989-01-01

    Spin-polarized energy-band calculations, including spin-orbit coupling in the band Hamiltonian, have been performed on CeRh3B2. Good agreement is obtained between theory and experiment concerning the magnetic moment. It is also found that the magnetic moment varies strongly with volume and from this a qualitative explanation for the anomalously high Curie temperature of the compound is proposed. Furthermore, the spin density is found to be highly nonspherical.

  16. Itinerant magnetism in CeRh3B2

    Science.gov (United States)

    Eriksson, Olle; Johansson, Börje; Brooks, M. S. S.; Skriver, H. L.; Sjöström, Jörgen

    1989-09-01

    Spin-polarized energy-band calculations, including spin-orbit coupling in the band Hamiltonian, have been performed on CeRh3B2. Good agreement is obtained between theory and experiment concerning the magnetic moment. It is also found that the magnetic moment varies strongly with volume and from this a qualitative explanation for the anomalously high Curie temperature of the compound is proposed. Furthermore, the spin density is found to be highly nonspherical.

  17. AGARD Flight Test Instrumentation Series. Volume 1, Issue 2. Basic Principles of Flight Test Instrumentation Engineering (Introduction Generale aux Principles de Base de l’Instrumentation des Essais en Vol)

    Science.gov (United States)

    1994-03-01

    Vol. 15, 1982. 19. Torge , Wolfgang , Geodasie, Walter de Gruyter, Berlin, 1975. 20. "World Geodetic System 1984 (WGS84) - Auszug aus dem DMA...of 100 m/sec, for example, a time error of 10 msec may cause a position error of 1 m. 13.6 REFERENCES 13.1 Augath, Von Wolfgang and Klaus Kummer, "Die

  18. The R3B experiment at FAIR

    Energy Technology Data Exchange (ETDEWEB)

    Scheit, Heiko [TU Darmstadt (Germany)

    2012-07-01

    The experiment Reactions with Relativistic Radioactive Beams (R{sup 3}B) at the FAIR facility will be installed at the high-energy branch of the Super-FRS. It allows for kinematically complete nuclear reaction studies with short-lived radioactive ion beams in inverse kinematics, utilizing a wide beam energy range from about 100-1000 MeV/u covering the full mass range up to uranium. Experimental programs will address fundamental questions in nuclear structure and reaction physics, as well as nuclear astrophysics. Recently, after a long research and development phase, important milestones toward the realization of the R{sup 3}B experiment have been reached with the completion of the technical design reports for two core components of the R{sup 3}B setup: the NeuLAND high-resolution neutron time-of-flight spectrometer and the CALIFA Barrel, which serves as a {gamma}-ray spectrometer, calorimeter and, together with the Si-tracker, as a target-recoil detector. In addition, the construction of the superconducting large-acceptance dipole magnet R3B-GLAD, used for the magnetic rigidity analysis of beam-rapidity charged fragments, is well underway with an expected delivery at GSI in summer 2013.

  19. 2000 Johnston Site 3B-P

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Underwater Site 3B-P was established at Johnston Atoll by Dr. James Maragos, U.S. Fish & Wildlife Service, on July 3, 2000. With a start point (meter 0) at...

  20. Human kidney anion exchanger 1 interacts with kinesin family member 3B (KIF3B)

    Energy Technology Data Exchange (ETDEWEB)

    Duangtum, Natapol [Medical Molecular Biology Unit, Office for Research and Development Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700 (Thailand); Department of Anatomy, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700 (Thailand); Junking, Mutita; Sawasdee, Nunghathai [Medical Molecular Biology Unit, Office for Research and Development Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700 (Thailand); Cheunsuchon, Boonyarit [Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700 (Thailand); Limjindaporn, Thawornchai, E-mail: limjindaporn@yahoo.com [Department of Anatomy, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700 (Thailand); Yenchitsomanus, Pa-thai, E-mail: grpye@mahidol.ac.th [Medical Molecular Biology Unit, Office for Research and Development Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700 (Thailand)

    2011-09-16

    Highlights: {yields} Impaired trafficking of kAE1 causes distal renal tubular acidosis (dRTA). {yields} The interaction between kAE1 and kinesin family member 3B (KIF3B) is reported. {yields} The co-localization between kAE and KIF3B was detected in human kidney tissues. {yields} A marked reduction of kAE1 on the cell membrane was observed when KIF3B was knockdown. {yields} KFI3B plays an important role in trafficking of kAE1 to the plasma membrane. -- Abstract: Impaired trafficking of human kidney anion exchanger 1 (kAE1) to the basolateral membrane of {alpha}-intercalated cells of the kidney collecting duct leads to the defect of the Cl{sup -}/HCO{sub 3}{sup -} exchange and the failure of proton (H{sup +}) secretion at the apical membrane of these cells, causing distal renal tubular acidosis (dRTA). In the sorting process, kAE1 interacts with AP-1 mu1A, a subunit of AP-1A adaptor complex. However, it is not known whether kAE1 interacts with motor proteins in its trafficking process to the plasma membrane or not. We report here that kAE1 interacts with kinesin family member 3B (KIF3B) in kidney cells and a dileucine motif at the carboxyl terminus of kAE1 contributes to this interaction. We have also demonstrated that kAE1 co-localizes with KIF3B in human kidney tissues and the suppression of endogenous KIF3B in HEK293T cells by small interfering RNA (siRNA) decreases membrane localization of kAE1 but increases its intracellular accumulation. All results suggest that KIF3B is involved in the trafficking of kAE1 to the plasma membrane of human kidney {alpha}-intercalated cells.

  1. LAND/R3B DAQ developments

    Energy Technology Data Exchange (ETDEWEB)

    Toernqvist, Hans; Aumann, Thomas; Loeher, Bastian [Technische Universitaet Darmstadt, Darmstadt (Germany); Simon, Haik [GSI Helmholtzzentrum fuer Schwerionenforschung GmbH, Darmstadt (Germany); Johansson, Haakan [Chalmers Institute of Technology, Goeteborg (Sweden); Collaboration: R3B-Collaboration

    2015-07-01

    Existing experimental setups aim to exploit most of the improved capabilities and specifications of the upcoming FAIR facility at GSI. Their DAQ designs will require some re-evaluation and upgrades. This presentation summarizes the R3B experimental campaigns in 2014, where the R3B DAQ was subject to testing of several new features that will aid researchers in using larger and more complicated experimental setups in the future. It also acted as part of a small testing ground for the NUSTAR DAQ infrastructure. In order to allow to extract correlations between several experimental sites, new suggested triggering and timestamping implementations were tested over significant distances. Also, with growing experimental complexity comes a greater risk of problems that may be difficult to characterize and solve. To this end, essential remote monitoring and debugging tools have been used successfully.

  2. Mathematics, Vol. 2.

    Science.gov (United States)

    Bureau of Naval Personnel, Washington, DC.

    The second of three volumes of a mathematics training course for Navy personnel, this document contains material primarily found at the college level. Beginning with logarithms and trigonometry, the text moves into vectors and static equilibrium (physics). Coordinate geometry, conic sections, and the tangents, normals, and slopes of curves follow.…

  3. Apache, Vol. I.

    Science.gov (United States)

    Bodiroga, Ronald, Comp.; And Others

    The short stories, poems, and cultural descriptions about American Indians (volume 1 of two) are the result of a combined effort of the 7th grade students of Rice School District (Sacaton, Arizona), their teacher, and the parents and friends of the students of the San Carlos Apache Indian Reservation (Arizona). The 24 student contributors prepared…

  4. Apache, Vol. II.

    Science.gov (United States)

    Bodiroga, Ronald, Comp.; And Others

    The short stories, poems, songs, and cultural descriptions about American Indians (volume 2 of two) are the result of a combined effort of the 7th grade students of Rice School District (Sacaton, Arizona), their teacher, and the parents and friends of the students of the San Carlos Apache Indian Reservation (Arizona). The 24 student contributors…

  5. A novel DNMT3B subfamily, DeltaDNMT3B, is the predominant form of DNMT3B in non-small cell lung cancer.

    Science.gov (United States)

    Wang, Luo; Wang, Jie; Sun, Shiyong; Rodriguez, Marivonne; Yue, Ping; Jang, Se Jin; Mao, Li

    2006-07-01

    De novo promoter DNA methylation represses gene transcription and is a common mechanism to inactivate tumor suppressor genes in tumorigenesis. DNMT3B plays an important role in de novo DNA methylation. We report here the identification of a novel DNMT3B subfamily, termed DeltaDNMT3B, whose expression is initiated through a promoter located at intron 4 and exon 5 of the DNMT3B gene. At least 7 transcriptional variants of DeltaDNMT3B have been observed as the result of alternative pre-mRNA splicing. Predicted proteins derived from these variants suggest that 4 of the variants share a conservative enzymatic domain but contain a variable PWWP motif, a putative DNA binding structure, whereas 3 of the variants lack the enzymatic domain due to predicted premature translational termination. In non-small cell lung cancer (NSCLC) cell lines, DeltaDNMT3B variants are frequently expressed and are the predominant forms of DNMT3B. Similarly, DeltaDNMT3B variants are frequently expressed in primary NSCLC but are not detectable or are expressed at low levels in corresponding normal lung tissue. Our results indicate that DeltaDNMT3B is the major expression form of DNMT3B in NSCLC and may play an important role in the development of aberrant promoter methylation during lung tumorigenesis.

  6. Discoloration and mineralization of Reactive Red HE-3B by heterogeneous photo-Fenton reaction.

    Science.gov (United States)

    Feng, Jiyun; Hu, Xijun; Yue, Po Lock; Zhu, Huai Yong; Lu, Gao Qing

    2003-09-01

    Discoloration and mineralization of Reactive Red HE-3B were studied by using a laponite clay-based Fe nanocomposite (Fe-Lap-RD) as a heterogeneous catalyst in the presence of H2O2 and UV light. Our experimental results clearly indicate that Fe-Lap-RD mainly consists of Fe2O3 (meghemite) and Fe2Si4O10(OH)2 (iron silicate hydroxide) which have tetragonal and monoclinic structures, respectively, and has a high specific surface area (472 m2/g) as well as a high total pore volume (0.547 cm3/g). It was observed that discoloration of HE-3B undergoes a much faster kinetics than mineralization of HE-3B. It was also found that initial HE-3B concentration, H2O2 concentration, UV light wavelength and power, and Fe-Lap-RD catalyst loading are the four main factors that can significantly influence the mineralization of HE-3B. At optimal conditions, complete discoloration of 100 mg/L HE-3B can be achieved in 30 min and the total organic carbon removal ratio can attain 76% in 120 min, illustrating that Fe-Lap-RD has a high photo-catalytic activity in the photo-assisted discoloration and mineralization of HE-3B in the presence of UV light (254 nm) and H2O2.

  7. Vibration Theory, Vol. 1B

    DEFF Research Database (Denmark)

    Asmussen, J. C.; Nielsen, Søren R. K.

    The present collection of MATLAB exercises has been published as a supplement to the textbook, Svingningsteori, Bind 1 and the collection of exercises in Vibration theory, Vol. 1A, Solved Problems. Throughout the exercise references are made to these books. The purpose of the MATLAB exercises is ...... is to give a better understanding of the physical problems in linear vibration theory and to surpress the mathematical analysis used to solve the problems. For this purpose the MATLAB environment is excellent....

  8. Splice variants DNMT3B4 and DNMT3B7 overexpression inhibit cell proliferation in 293A cell line.

    Science.gov (United States)

    Shao, Guo; Zhang, Ran; Zhang, Shu; Jiang, Shuyuan; Liu, You; Zhang, Wei; Zhang, Yanbo; Li, Jinping; Gong, Kerui; Gong, Keri; Hu, Xin-Rong; Jiang, Shi-Wen

    2013-05-01

    DNA methyltransferase 3B (DNMT3B) is critical in abnormal DNA methylation patterns in cancer cells. Nearly 40 alternatively spliced variants of DNMT3B have been reported. DNMT3B4 and DNMT3B7 are two kinds of splice variants of DNMT3B lacking the conserved methyltransferase motif. In this study, the effect of inactivation of DNMT3B variants, DNMT3B4 and DNMT3B7, on cell proliferation was assessed. pCMV-DNMT3B4 and pCMV-DNMT3B7 recombinant plasmids were developed and stably transfected into 293A cells. 293A cells transfected with plasmid pCMV-DNMT3B4 or pCMV-2B were then treated with G418 to the stable cell lines. After that, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method was used for testing the proliferation level, and flow cytometry was used to test cell cycle distribution of the cell line. The expression of p21 was detected by real-time PCR and Western blot. The methylation status of p21 promoter was detected by methylation-specific PCR (MS-PCR). It was found that DNMT3B4 and DNMT3B7 overexpression could inhibit cell proliferation and increase the expression of p21. Cell cycle analysis demonstrated that inactivation of DNMT3B variants overexpression inhibited cell cycle progression. Inactivation of DNMT3B variants overexpression facilitated p21 expression to delay 293A cell proliferation. These findings indicate that inactivation of DNMT3B variants might play an important role in cell proliferation correlating with the change of p21.

  9. Leptin receptor expressing neurons express phosphodiesterase-3B (PDE3B) and leptin induces STAT3 activation in PDE3B neurons in the mouse hypothalamus.

    Science.gov (United States)

    Sahu, Maitrayee; Sahu, Abhiram

    2015-11-01

    Leptin signaling in the hypothalamus is critical for normal food intake and body weight regulation. Cumulative evidence suggests that besides the signal transducer and activator of transcription-3 (STAT3) pathway, several non-STAT3 pathways including the phosphodiesterase-3B (PDE3B) pathway mediate leptin signaling in the hypothalamus. We have shown that PDE3B is localized in various hypothalamic sites implicated in the regulation of energy homeostasis and that the anorectic and body weight reducing effects of leptin are mediated by the activation of PDE3B. It is still unknown if PDE3B is expressed in the long form of the leptin-receptor (ObRb)-expressing neurons in the hypothalamus and whether leptin induces STAT3 activation in PDE3B-expressing neurons. In this study, we examined co-localization of PDE3B with ObRb neurons in various hypothalamic nuclei in ObRb-GFP mice that were treated with leptin (5mg/kg, ip) for 2h. Results showed that most of the ObRb neurons in the arcuate nucleus (ARC, 93%), ventromedial nucleus (VMN, 94%), dorsomedial nucleus (DMN, 95%), ventral premammillary nucleus (PMv, 97%) and lateral hypothalamus (LH, 97%) co-expressed PDE3B. We next examined co-localization of p-STAT3 and PDE3B in the hypothalamus in C57BL6 mice that were treated with leptin (5mg/kg, ip) for 1h. The results showed that almost all p-STAT3 positive neurons in different hypothalamic nuclei including ARC, VMN, DMN, LH and PMv areas expressed PDE3B. These results suggest the possibility for a direct role for the PDE3B pathway in mediating leptin action in the hypothalamus. Copyright © 2015 Elsevier Inc. All rights reserved.

  10. Fração de ejeção e volumes do ventrículo esquerdo medidos com eco 3D e com tomografia ultra-rápida Fracción de eyección y volúmenes del ventrículo izquierdo medidos con eco- 3DTR y con tomografía ultrarrápida Left ventricular ejection fraction and volumes as measured by 3d echocardiography and ultrafast computed tomography

    Directory of Open Access Journals (Sweden)

    Marcelo Luiz Campos Vieira

    2009-04-01

    Full Text Available FUNDAMENTO: O ecocardiograma tridimensional em tempo real (ECO 3D e a tomografia computadorizada ultra-rápida (CT são dois novos métodos de análise da fração de ejeção e dos volumes do VE. OBJETIVO: Comparar as medidas da FEVE e dos volumes do VE aferidos pelo ECO 3D e pela CT ultra-rápida. MÉTODOS: Foram estudados pelo ECO 3D e pela CT ultra-rápida de 64 cortes, 39 pacientes consecutivos (27 homens, média etária de 57±12 anos. Foram analisados: FEVE e volumes do VE. Análise estatística: coeficiente de correlação (r: Pearson, teste de Bland & Altman, teste de regressão linear, 95 % IC, pFUNDAMENTO: La ecocardiografía tridimensional en tiempo real (Eco-3DTR y la tomografía computarizada ultrarrápida (TC ultrarrápida son dos nuevos métodos de análisis de la fracción de eyección (FE y de los volúmenes del ventrículo izquierdo (VI. OBJETIVO: Comparar las mediciones de la fracción de eyección del ventrículo izquierdo (FEVI y de los volúmenes del VI apurados por la Eco-3DTR y por la TC ultrarrápida. MÉTODOS: Se estudiaron, mediante la Eco-3DTR y la TC ultrarrápida de 64 cortes, a 39 pacientes consecutivos (27 varones, promedio de edad de 57±12 años. Se analizaron: FEVI y volúmenes del VI. Análisis estadístico: coeficiente de correlación (r: Pearson, prueba de Bland & Altman, prueba de regresión lineal, 95 % IC, pBACKGROUND: Real-time three-dimensional echocargiography (RT-3D-Echo and ultrafast computed tomography (CT are two novel methods for the analysis of LV ejection fraction and volumes. OBJECTIVE: To compare LVEF and volume measurements as obtained using RT-3D-Echo and ultrafast CT. METHODS: Thirty nine consecutive patients (27 men, mean age of 57±12 years were studied using RT-3D-Echo and 64-slice ultrafast CT. LVEF and LV volumes were analyzed. Statistical analysis: coefficient of correlation (r: Pearson, Bland-Altman analysis, linear regression analysis, 95% CI, p<0.05. RESULTS: RT-3D

  11. Truncated DNMT3B isoform DNMT3B7 suppresses growth, induces differentiation, and alters DNA methylation in human neuroblastoma

    OpenAIRE

    Ostler, Kelly R.; Yang, Qiwei; Looney, Timothy J.; Li ZHANG; Vasanthakumar, Aparna; Tian, Yufeng; Kocherginsky, Masha; Stacey L. Raimondi; DeMaio, Jessica G.; Salwen, Helen R.; Gu, Song; Chlenski, Alexandre; Naranjo, Arlene; Gill, Amy; Peddinti, Radhika

    2012-01-01

    Epigenetic changes in pediatric neuroblastoma may contribute to the aggressive pathophysiology of this disease, but little is known about the basis for such changes. In this study, we examined a role for the DNA methyltransferase DNMT3B, in particular, the truncated isoform DNMT3B7 which is generated frequently in cancer. To investigate if aberrant DNMT3B transcripts alter DNA methylation, gene expression, and phenotypic character in neuroblastoma, we measured DNMT3B expression in primary tum...

  12. Analysis list: Sin3b [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Sin3b Muscle + mm9 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Sin3b.1.ts...v http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Sin3b.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Si...n3b.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Sin3b.Muscle.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Muscle.gml ...

  13. Broadcasting Satellite-3A and -3B (BS-3A and 3B)

    Science.gov (United States)

    Horii, M.; Funakawa, K.

    1991-01-01

    The BS-3A and -3B will provide direct color TV broadcasting to the Japanese mainland and remote islands. The satellites will be launched from Tanegashima Space Center by a type H-1 launch vehicle. The coverage will consist of the 26-m antenna and the 34-m antenna as a backup support for the transfer and drift orbits. Maximum support will consist of one 8-hour track per station for a seven day period, plus 23 days of contingency support from all complexes. Information is given in tabular form for Deep Space Network support, frequency assignments, telemetry, command, and tracking support responsibility.

  14. Magnetic and NMR investigation of RRh3B2 (R=La to Gd) compounds

    Science.gov (United States)

    Malik, S. K.; Dhar, S. K.; Vijayaraghavan, R.; Wallace, W. E.

    1982-11-01

    Magnetization (4.2 to 300 K) and 11B paramagnetic NMR (77 to 300 K) studies have been carried out on ternary borides RRh3B2 (R=La to Gd). These compounds crystallize in the CeCo3B2-type structure. From cell volume considerations Ce is in a mixed valent/quadrivalent state while the rest of the rare earth ions are in a trivalent state. With the exception of La and Pr compounds, all other compounds are found to order magnetically. From the high values of the ratio of paramagnetic moment to ordered state moment, the magnetic ordering is inferred to be the itinerant type in CeRh3B2 and EuRh3B2. In Nd and Gd compounds, magnetization data reveal that magnetic ordering is associated with the rare earth sublattice. A complex behavior of magnetization is observed in SmRh3B2. A first order quadrupole split 11B NMR spectrum is observed in Ce, Pr, Nd, and Sm compounds. The boron Knight shift in all these compounds is small and, within the limits of experimental error, scales linearly with susceptibility in Pr and Nd compounds. A temperature independent Knight shift is observed in SmRh3B2, indicating the influence of strong crystal fields.

  15. 季节、品种对种公猪精液量及精子活力的影响%Effects of Season and Va-riety on the Semen Vol-ume and Sperm Motility of Breeding Boar

    Institute of Scientific and Technical Information of China (English)

    姚建军

    2014-01-01

    The research aimed to study the effects of season and variety on the semen volume and sperm motility of adult boar. [Method] The se-men volume and sperm motility of 21 Landrace pigs and 26 Yorkshire pigs in different seasons were observed and de-termined. And the statistical analysis was made on these quality indices of semen. [Result] The effects of different seasons on the semen volume of adult boar reached significant level (P0.05). The semen volume of Landrace pigs in spring was the most, and that in autumn, winter and summer was decreased in succession. The semen volume of York-shire pigs in autumn was the most, and that in summer, spring and winter was decreased in succession. The effects of season and variety on the sperm motility of adult boar were not significant (P>0.05), but the sperm motility of Landrace pigs and Yorkshire pigs reached the maximum values in spring and reached the minimum values in summer. Further-more, the sperm motility of Landrace pigs in four seasons was obviously higher than that of Yorkshire pigs. [Conclusion] The results laid the foundation for im-proving the reproductive efficiency and production level of pig farms.%[目的]研究季节、品种两大因素对成年公猪精液量及精子活力的影响效应。[方法]对21头长白猪和26头大白猪在一年内不同季节的精液量和精子活力进行观察和测定,并对这些精液品质进行统计学分析。[结果]季节对成年公猪精液量的影响达到显著水平(P0.05),其中长白猪的精液量在春季最多,秋、冬、夏依次减少,而大白猪的精液量在秋季最多,夏、春、冬三季则依次减少;季节和品种对成年公猪精子活力的影响均不显著(P>0.05),但是长白猪和大白猪的精子活力均在春季最高,而夏季最低,此外一年四季长白猪的精子活力明显高于大白猪。[结论]结果为提高猪场的繁殖效率和生产水平奠定基础。

  16. MANHATTAN DISTRICT HISTORY PROJECT Y THE LOS ALAMOS PROJECT VOL. I INCEPTION UNTIL AUGUST 1945

    Energy Technology Data Exchange (ETDEWEB)

    Hawkins, D.

    1961-12-01

    THESE TWO VOLUMES CONSTITUTE A RECORD OF THE TECHNICAL, ADMINISTRATIVE , AND POLICY-MAKING ACTIVITIES OF THE LOS ALAMOS PROJECT (PROJECT Y) FROM ITS INCEPTION UNDER THE MANHATTAN DISTRICT THROUGH THE DEVELOPMENT OF THE ATOMIC BOMB (VOL. I), AND DURING THE PERIOD FOLLOWING THE END OF WORLD WAR II UNTIL THE MANHATTAN DISTRICT RELINQUISHED CONTROL TO THE ATOMIC ENERGY COMMISSION AS OF JANUARY 1947 (VOL. II). ALTHOUGH SECURITY REGULATIONS HAVE REQUIRED SOME DELETIONS IN THE ORIGINAL TEXT OF THE TWO VOLUMES, EVERY EFFORT HAS BEEN MADE TO RETAIN THE ORIGINAL LANGUAGE AND EXPRESSIONS OF THE AUTHORS.

  17. MANHATTAN DISTRICT HISTORY PROJECT Y THE LOS ALAMOS PROJECT VOL. II AUGUST 1945 THROUGH DECEMBER 1946

    Energy Technology Data Exchange (ETDEWEB)

    Truslow, E. C.; Smith, R. C.

    1961-12-01

    THESE TWO VOLUMES CONSTITUTE A RECORD OF THE TECHNICAL, ADMINISTRATIVE , AND POLICY-MAKING ACTIVITIES OF THE LOS ALAMOS PROJECT (PROJECT Y) FROM ITS INCEPTION UNDER THE MANHATTAN DISTRICT THROUGH THE DEVELOPMENT OF THE ATOMIC BOMB (VOL. I), AND DURING THE PERIOD FOLLOWING THE END OF WORLD WAR II UNTIL THE MANHATTAN DISTRICT RELINQUISHED CONTROL TO THE ATOMIC ENERGY COMMISSION AS OF JANUARY 1947 (VOL. II). ALTHOUGH SEC URITY REGULATIONS HAVE REQUIRED SOME DELETIONS IN THE ORIGINAL TEXT OF THE TWO VOLUMES, EVERY EFFORT HAS BEEN MADE TO RETAIN THE ORIGINAL LANGUGAGE AND EXPERSSIONS OF THE AUTHORS.

  18. DNMT3B7, a truncated DNMT3B isoform expressed in human tumors, disrupts embryonic development and accelerates lymphomagenesis

    Science.gov (United States)

    Shah, Mrinal Y.; Vasanthakumar, Aparna; Barnes, Natalie Y.; Figueroa, Maria E.; Kamp, Anna; Hendrick, Christopher; Ostler, Kelly R.; Davis, Elizabeth M.; Lin, Shang; Anastasi, John; Le Beau, Michelle M.; Moskowitz, Ivan; Melnick, Ari; Pytel, Peter; Godley, Lucy A.

    2010-01-01

    Epigenetic changes are among the most common alterations observed in cancer cells, yet the mechanism by which cancer cells acquire and maintain abnormal DNA methylation patterns is not understood. Cancer cells have an altered distribution of DNA methylation and express aberrant DNA methyltransferase 3B transcripts, which encode truncated proteins, some of which lack the C-terminal catalytic domain. To test if a truncated DNMT3B isoform disrupts DNA methylation in vivo, we constructed two lines of transgenic mice expressing DNMT3B7, a truncated DNMT3B isoform commonly found in cancer cells. DNMT3B7 transgenic mice exhibit altered embryonic development, including lymphopenia, craniofacial abnormalities, and cardiac defects, similar to Dnmt3b-deficient animals, but rarely develop cancer. However, when DNMT3B7 transgenic are bred with Eμ-Myc transgenic mice, which model aggressive B cell lymphoma, DNMT3B7 expression increases the frequency of mediastinal lymphomas in Eμ-Myc animals. Eμ-Myc/DNMT3B7 mediastinal lymphomas have more chromosomal rearrangements, increased global DNA methylation levels, and more locus-specific perturbations in DNA methylation patterns compared to Eμ-Myc lymphomas. These data represent the first in vivo modeling of cancer-associated DNA methylation changes and suggest that truncated DNMT3B isoforms contribute to the re-distribution of DNA methylation characterizing virtually every human tumor. PMID:20587527

  19. AGARD Flight Test Techniques Series. Volume 11. The Testing of Fixed Wing Tanker and Receiver Aircraft to Establish Their Air-To-Air Refuelling Capabilities (Les Essais Pratiques sur les Avions Ravitailleurs et Ravitailles afin de Determiner leurs Capacites de Ravitaillement en Vol).

    Science.gov (United States)

    1992-12-01

    doivent etre pris en compte lors de la planification des essais de ravitaillement en vol d’un nouvel avion-citerne ou d’un nouvel avion ä ravitailler...en vue de son homologation. Le lecteur est suppose etre familier avec la pratique courante de ravitaillement en vol et le materiel employes dans

  20. Significance of DNMT3b in oral cancer.

    Directory of Open Access Journals (Sweden)

    Wen-Cheng Chen

    Full Text Available The aim of this study was to explore specific molecular markers that could lead to new insights into the identification of innovative treatments. The role of DNMT3b and its predictive power in the prognosis of oral cancer were identified. Human oral cancer cell lines including SCC4 and SCC25 were selected for cellular experiments. Changes in tumor growth, aggressiveness and the responsible signaling pathway were investigated in vitro and in vivo. Furthermore, 125 oral cancer tissue specimens were analyzed using immunohistochemical staining on tissue microarray slides, and correlations calculated between the level of DNMT3b and the clinical outcome of patients. Our data revealed that inhibition of DNMT3b resulted in slower tumor growth, attenuated tumor invasion ability and epithelial mesenchymal transition, as determined by in vitro and in vivo experiments. Activated IL-6 signaling might be responsible to the induction of DNMT3b overexpression on oral cancer. Regarding clinical data, the incidence of DNMT3b immunoreactivity in oral cancer specimens was significantly higher than in non-malignant epithelium, and positively linked to expression of IL-6. Furthermore, expression of DNMT3b was significantly linked with the risk of lymph node involvement, disease recurrence and shorter survival in patients with pathological stage III-IV oral cancer. In conclusion, IL-6 -DNMT3b axis could be used to predict the prognosis of oral cancer in clinics, and targeting DNMT3b could represent a promising treatment strategy.

  1. Significance of DNMT3b in oral cancer.

    Science.gov (United States)

    Chen, Wen-Cheng; Chen, Miao-Fen; Lin, Paul-Yang

    2014-01-01

    The aim of this study was to explore specific molecular markers that could lead to new insights into the identification of innovative treatments. The role of DNMT3b and its predictive power in the prognosis of oral cancer were identified. Human oral cancer cell lines including SCC4 and SCC25 were selected for cellular experiments. Changes in tumor growth, aggressiveness and the responsible signaling pathway were investigated in vitro and in vivo. Furthermore, 125 oral cancer tissue specimens were analyzed using immunohistochemical staining on tissue microarray slides, and correlations calculated between the level of DNMT3b and the clinical outcome of patients. Our data revealed that inhibition of DNMT3b resulted in slower tumor growth, attenuated tumor invasion ability and epithelial mesenchymal transition, as determined by in vitro and in vivo experiments. Activated IL-6 signaling might be responsible to the induction of DNMT3b overexpression on oral cancer. Regarding clinical data, the incidence of DNMT3b immunoreactivity in oral cancer specimens was significantly higher than in non-malignant epithelium, and positively linked to expression of IL-6. Furthermore, expression of DNMT3b was significantly linked with the risk of lymph node involvement, disease recurrence and shorter survival in patients with pathological stage III-IV oral cancer. In conclusion, IL-6 -DNMT3b axis could be used to predict the prognosis of oral cancer in clinics, and targeting DNMT3b could represent a promising treatment strategy.

  2. Increased DNA methylation of Dnmt3b targets impairs leukemogenesis.

    Science.gov (United States)

    Schulze, Isabell; Rohde, Christian; Scheller-Wendorff, Marina; Bäumer, Nicole; Krause, Annika; Herbst, Friederike; Riemke, Pia; Hebestreit, Katja; Tschanter, Petra; Lin, Qiong; Linhart, Heinz; Godley, Lucy A; Glimm, Hanno; Dugas, Martin; Wagner, Wolfgang; Berdel, Wolfgang E; Rosenbauer, Frank; Müller-Tidow, Carsten

    2016-03-24

    The de novo DNA methyltransferases Dnmt3a and Dnmt3b are of crucial importance in hematopoietic stem cells. Dnmt3b has recently been shown to play a role in genic methylation. To investigate how Dnmt3b-mediated DNA methylation affects leukemogenesis, we analyzed leukemia development under conditions of high and physiological methylation levels in a tetracycline-inducible knock-in mouse model. High expression of Dnmt3b slowed leukemia development in serial transplantations and impaired leukemia stem cell (LSC) function. Forced Dnmt3b expression induced widespread DNA hypermethylation inMyc-Bcl2-induced leukemias, preferentially at gene bodies.MLL-AF9-induced leukemogenesis showed much less pronounced DNA hypermethylation upon Dnmt3b expression. Nonetheless, leukemogenesis was delayed in both models with a shared core set of DNA hypermethylated regions and suppression of stem cell-related genes. Acute myeloid leukemia patients with high expression of Dnmt3b target genes showed inferior survival. Together, these findings indicate a critical role for Dnmt3b-mediated DNA methylation in leukemia development and maintenance of LSC function.

  3. The Writing Workshop. Vol. 2.

    Science.gov (United States)

    Ziegler, Alan

    The second of two volumes, this book is primarily concerned with the teaching of creative writing at any grade level, although some ideas may not be applicable to teaching very young students. This volume focuses on how to generate writing assignments, and while it is designed for classroom teachers, it can also be used by individual writers.…

  4. Reposição volêmica intraoperatória: cristaloides versus coloides em revascularização cirúrgica do miocárdio sem circulação extracorpórea Reposición volémica intraoperatoria: cristaloides versus coloides en revascularización quirúrgica del miocardio sin circulación extracorpórea Intraoperative volume replacement: crystalloids versus colloids in surgical myocardial revascularization without cardiopulmonary bypass

    Directory of Open Access Journals (Sweden)

    Raquel Reis Soares

    2009-08-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: O uso de cristaloides ou coloides na reposição volêmica de intervenções cirúrgicas de grande porte é assunto controverso. O objetivo deste trabalho foi comparar os efeitos do cristaloide (solução fisiológica a 0,9% SF com coloide (gelatina fluida modificada quando administrados no intraoperatório de revascularização cirúrgica do miocárdio (RVCM sem circulação extracorpórea (CEC. MÉTODO: Quarenta pacientes submetidos à RVCM sem CEC foram divididos aleatoriamente em dois grupos similares. O primeiro grupo recebeu gelatina fluida modificada e SF e o segundo grupo recebeu somente SF. Registrou-se a diurese, nível da hemoglobina, sangramentos intra e pós-operatórios, valores de glicemia e lactato do intraoperatório em quatro medidas distintas. Foram avaliados a morbimortalidade pós-operatória, o tempo de internação na unidade de terapia intensiva (UTI e o tempo de internação hospitalar. RESULTADOS: O tempo de extubação do grupo da gelatina foi de 6,6 horas contra 7,3 horas do grupo do SF. O tempo de internação no CTI foi de 2,4 dias no grupo da gelatina contra 3,3 dias no grupo do SF. O tempo de internação hospitalar no grupo da gelatina foi de 10,3 dias contra 6,8 dias no grupo do uso exclusivo de SF. A ocorrência de complicações renais, respiratórias, disritmias cardíacas, infartos, infecções, reintubações, transfusões sanguíneas e reoperações foi a mesma. CONCLUSÕES: O uso de coloides, representados pela gelatina fluida modificada associada a cristaloides ou o uso de cristaloides exclusivamente não alterou o prognóstico pós-operatório de pacientes submetidos à RVCM sem CEC nos pacientes estudados. Talvez mais importante que o tipo de líquido administrado ao paciente cirúrgico seja a manutenção de estabilidade hemodinâmica adequada durante o procedimento.JUSTIFICATIVA Y OBJETIVOS: El uso de cristaloides o coloides en la reposición volémica de intervenciones

  5. Simple groups with orders 2a3b5cpd,2a3b7cpd and 2a3b5c7d

    Institute of Scientific and Technical Information of China (English)

    JIANG Youyi; TAN Mingshu; LIU Xuefei

    2004-01-01

    This work deals with the power exponent r1 and r2 respectively of the maximal and second-maximal prime factors of the order of simple K4-group, and the classification for simple {5,7}'- K4-group G (i.e. |G| can not be divided by 5 nor by 7 or |π(G)| = 4 ), simple 5' - K4 -group G (i.e. |G| can not divided by 5 and |π(G)| =4) and simple 7'- K4 -group G (i.e. |G| can not divided by 7 and |π(G)| =4). It is derived that r1 =1, 2 and 4, and r2 is not greater than 4. All the simple K4 -groups with order 2a3b5cpd, 2a3b7cpd and 2a3b5c7d are obtained.

  6. Determinación de volúmenes pulmonares mediante pletismografía en pacientes con fíbrosis quística Lung volume determinations by whole plethysmography in cystic fibrosis patients: A preliminary report

    Directory of Open Access Journals (Sweden)

    ILSE CONTRERAS E

    2008-03-01

    Full Text Available Introducción: La fibrosis quística (FQ exhibe un deterioro progresivo de la función pulmonar (FP asociada a infecciones recurrentes. Objetivo: Evaluar la FP en sujetos con FQ mediante espirometría, saturometría y pletismografía (PG determinando alguna relación matemática. Pacientes y Métodos: Se identificaron 10 pacientes capaces de realizar CVF, CRF, VR, CPT, VEF1, y FEF25-75 %; comparándose con los valores de Zapletal y de 33 controles sanos. Resultados: Edad promedio 13,6 años (rango: 9-20. Sa0(2y VEF1fueron normales en 10 y 5 sujetos, respectivamente (4 tenían VEF1entre 65-80%. La PG mostró 4 pacientes con VR/CPT > 30%, 3 con aumento de CRF y VR/CPT, sólo uno tuvo PG normal. Los promedios de CPT y VR fueron superiores a valores de referencia y controles (p Introduction: Cystic fibrosis (CF patients develop a progressive impairment of pulmonary function tests (PFT by recurrent infection. Objective: To evaluate PFT in CF patients using spirometry, oxygen saturometry (Sa0(2 and whole plethysmography (WP for determining possible mathematical relationships. Methods: FVC, FRC, RV, TLC, FEV1, FEF25.75, obtained in 10 CF patients were compared with Zapletal's reference values and with the values obtained in 33 healthy-school children. Results: Mean age 13.6 years-old (range: 9-20. All CF patients had normal Sa0(2, FEV1 was normal in five (four had FEV1 between 65-80%, 4 had RV/TLC > 30%, 3 had increased FRC and RV/TLC, only one CF patient had WP normal. The average of TLC and RV were higher than reference values and control group (p < 0.05. We found an inverse relationship between FEV1, FEF25-75 and RV/TLC [r = -0.642 (p < 0.05 and r = -0.803 (p < 0.01; respectively]. Conclusion: This study suggests an increase of pulmonary volume in CF patients with not necessarily abnormal spirometry and an inverse mathematical relationship between FEV1, FEF25-75 and RV/TLC

  7. Osmosis and Surface Area to Volume Ratio.

    Science.gov (United States)

    Barrett, D. R. B.

    1984-01-01

    Describes an experiment designed to help students understand the concepts of osmosis and surface area to volume ratio (SA:VOL). The task for students is to compare water uptake in different sizes of potato cubes and relate differences to their SA:VOL ratios. (JN)

  8. APOBEC3B expression in human leptomeninges and meningiomas

    Science.gov (United States)

    Johnson, Mahlon D.; Reeder, Jay E.; O'Connell, Mary

    2016-01-01

    Nucleic acid-editing enzymes of the apolipoprotein B mRNA-editing enzyme (APOBEC) family have been associated with somatic mutation in cancer. However, the role of APOBEC catalytic subunit 3B (APOBEC3B) editing in the pathogenesis of base substitutions in meningiomas is unknown. In the present study, the expression of APOBEC3B was examined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analyses in five fetal and one adult human leptomeninges and 38 meningiomas. Genomic DNA was sequenced using the Illumina Tru-Seq Cancer Panel. Three meningioma primary cultures were also established and treated with cerebrospinal fluid form patients without neurological disease or platelet-derived growth factor-BB (PDGF-BB), prior to evaluation of APOBEC3B expression. By western blotting, APOBEC3B was revealed to be present in 100% of the fetal leptomeninges, and in 88% of World Health Organization grade I, 100% of grade II and 83% of grade III meningiomas tested, but was not different between grades. RT-qPCR revealed no difference in the mRNA expression of APOBEC3B between grades. Sequencing revealed no elevated levels of the C>T mutations that are characteristic of APOBEC3B editing of genomic DNA. Treatment with cerebrospinal fluid and PDGF-BB had no effect on APOBEC3B protein expression in the leptomeningeal or meningioma cells. These findings suggest that the mutations associated with increased APOBEC3B expression may not be central to the pathogenesis of meningiomas. PMID:28101245

  9. Structural applications of Avimid K3B LDF thermoplastic composites

    Science.gov (United States)

    Perrella, Andrew P.

    Composite applications on advanced aircraft require lightweight, high performance, tough material systems which are capable of operating at high service temperatures. These composite systems must also be producible and cost effective. Avimid K3B composite materials and related process and part manufacturing technologies offers a unique solutions to these requirements. The objective of this paper is to describe selected Avimid K3B processing approaches such as Long Discontinuous Fiber thermoforming and fusion bonding. A review of the Avimid K3B F-16 Strake Door Joint Development Program is presented. This program successfully developed, built and structurally validated a flight demonstration component using these materials and manufacturing methods.

  10. Vol 38 2010 Art 6.pub

    African Journals Online (AJOL)

    p2613611

    ISSN 0378-5254 Journal of Family Ecology and Consumer Sciences, Vol 38, 2010 .... m only —foods which prom ote health beyond providing .... health conscious individuals may be motivated to process ...... Food industry forecast: Consumer.

  11. Vol 37 2009 Art 3.pub

    African Journals Online (AJOL)

    user

    ISSN 0378-5254 Journal of Family Ecology and Consumer Sciences, Vol 37, 2009. Young adult ..... decision making on products (Alch, 2005; Anderson et al, 2007; Berndt, .... gies during the shopping process (Y Monsuwe´ et al,. 2004).

  12. Maintenance of DNA methylation: Dnmt3b joins the dance.

    Science.gov (United States)

    Walton, Emma L; Francastel, Claire; Velasco, Guillaume

    2011-11-01

    DNA methylation mostly occurs within the context of CpG dinucleotides and is essential for embryonic development and gene repression. It is generally accepted that DNA methyltransferases carry out specific and non-overlapping functions, Dnmt3a and Dnmt3b being responsible for the establishment of methylation around the time of implantation and Dnmt1 ensuring that methylation is faithfully copied to daughter cells via what has come to be known as "maintenance methylation." This longstanding view has been challenged over the years with the observation that Dnmt1 alone is incapable of perfect maintenance methylation. A new model is emerging that takes into account a contribution of the de novo enzymes Dnmt3a and Dnmt3b in the maintenance of the DNA methylation. We recently showed that certain germ line genes are specific targets of Dnmt3b, and that Dnmt3b remains bound to their promoter regions in somatic cells via interaction with the transcriptional repressor E2F6. It is tempting to consider an ongoing role for Dnmt3b in the methylation of germ line genes in somatic cells. We propose here observations in support of the hypothesis that the maintenance of methylation and subsequent silencing of a handful of germ line genes requires Dnmt3b but not Dnmt1. In addition to suggesting a new role for Dnmt3b in the protection of somatic cells against the promiscuous expression of the germ line program, these observations are of particular interest in the field of carcinogenesis, given that the expression of catalytically inactive Dnmt3b isoforms and aberrant expression of germ line genes are commonly observed in cancer cells.

  13. LM-3B/E will launch Apstar 7

    Institute of Scientific and Technical Information of China (English)

    Zong He

    2009-01-01

    @@ China Great Wall Industry Corporation (CGWlC), a subsidiary of China Aerospace Science and Technology Corporation (CASC), signed a launch services contract with Hong Kong APT Satellite Co., Ltd in Beijing on November 8. According to the contract, a Long March 3B enhanced launch vehicle (LM-3B/E) will launch a French Thales Alenia Space made APstar 7 communications satellite into space in the first half year of 2012.

  14. ATF2 Proposal Vol.2

    OpenAIRE

    ATF2 Group

    2006-01-01

    The two major goals for this facility, called ATF2, are : (A) Achievement of a 37 nm beam size, and (B) control of beam position down to 2 nm level. The scientific justification for the ATF2 project and its technical design have been described in Volume 1 of the ATF2 Proposal. We present here Volume 2 of the ATF2 Proposal, in which we present specifics of the construction plans and the group organization to execute the research programs at ATF2.

  15. Possible antiferromagnetic interactions in NdRh 3B 2 and CeRh 3B 2

    Science.gov (United States)

    Takeda, K.; Kohara, T.; Kitaoka, Y.; Asayama, K.

    1987-06-01

    The magnetic phase transition and spin arrangement in the ordered phase of NdRh3B2 and CeRh3B2 have been studied by measurements of magnetic susceptibility and heat capacity of the isostructural compounds RRh3B2 ( R: La, Ce, Nd, Gd). The existence of small antiferromagnetic interactions between R ions ( R = Nd, Ce) on the neighbouring ferromagnetic chains along the c-axis has been suggested. This model gives another aspect to explain the origin of the anomalous magnetism of CeRh3B2 such as the small value of magnetic moment in the ordered state and the curious behaviour of susceptibility. The magnitude of the effective moment of Ce has been estimated for the consumption of magnetic entropy of the Ce compound.

  16. Volume replacement with hydroxyethyl starch or Ringer lactate solution in hemorrhagic gastroenteritis dogs with parvovirusReposição volêmica com hidroxietilamido ou solução de ringer lactato em cães com gastroenterite hemorrágica por parvovírus

    Directory of Open Access Journals (Sweden)

    Eduardo Ghiggi

    2013-09-01

    Full Text Available Parvovirus is an infectious viral disease, highly contagious, affecting dogs causing acute enteritis. Dehydration is a minor problem and acute evolution, which is the main focus of treatment. The objective of this study was to evaluate two treatments volume replacement in dogs with confirmed diagnosis of parvovirus infection by antigen detection in stool by ELISA. A total of 15 dogs, mixed, male and female, aged from 3 to 6 months, these four they had died during treatment and 11 were included in the study. Patients were randomly divided into two groups: hydroxyethyl starch 130/0.4 (HES, n=6 and Group Ringer Lactate (RL, n=5, which received fluid resuscitation with hydroxyethyl starch 130/0.4 and Ringer’s solution lactate, respectively, based on calculations of fluid deficit. All animals received intravenously: cephalothin, metronidazole, ranitidine and metoclopramide. During hospitalization, every 12 hours were evaluated arterial blood gas, electrolyte dosing and clinical examination (heart rate, respiratory rate, rectal temperature, capillary refill time, vomiting, appetite, dehydration, weight gain, hematochezia, melena, hematemesis and presence abdominal pain and 24 hours each blood count was performed. In group RL was increase in the values of PaO2 and decreases in hemoglobin, hematocrit, phosphorus and magnesium, and group HES, a decrease in the percentage of dehydration, hemoglobin, phosphorus and potassium. There were no differences in other parameters between the groups. Both treatments were effective in fluid replacement of patients, no difference in any variable analyzed. A parvovirose é uma doença infecciosa viral, altamente contagiosa, que acomete cães causando enterite aguda. A desidratação é um problema secundário e de evolução aguda, sendo este o foco principal do tratamento. O objetivo deste estudo foi avaliar dois tratamentos de reposição volêmica em cães com diagnóstico confirmado de parvovirose por detec

  17. Responsiveness to different volume therapies following hemodilution and hemorrhagic shock: a comparative experimental study in rats Resposta a diferentes terapias por reposição volêmica após hemodiluição e choque hemorrágico: estudo experimental comparativo em ratos

    Directory of Open Access Journals (Sweden)

    Riad Naim Younes

    2007-10-01

    Full Text Available Guidelines for volume replacement for acutely hemorrhaged and hemodiluted trauma patients have not been well established. Purpose: To evaluate the effects of acute hemodilution on mean arterial pressure (MAP, and responsiveness of acutely hemodiluted and subsequently hemorrhaged rats to different volume therapies. Methods: 180 rats were hemodiluted to simulate hemorrhaged trauma patients with persistent bleeding after high volume replacement with isotonic solutions. Thirty hemodiluted [Anemia (ANE group] animals received no further treatment. The remaining 150 animals were subjected to hypovolemic shock and randomized into five groups, according to the treatment option employed: Control (CTL animals did not receive subsequent treatment after hemorrhagic hypovolemia, SAL4 animals received isotonic saline 4 mL/kg, SAL32 animals received isotonic saline 32 mL/kg, HS animals received hypertonic saline 4 mL/kg and BLD animals received re-infusion of drawn blood. Results: Highest mean arterial pressure (MAP was achieved by BLD, followed by SAL32 and HS. MAP after treatment of BLD, HS, SAL32 and ANE were higher than CTL (p=0.036. At 85 and 95 minutes of experiment, SAL4, SAL32 and HS presented the lowest hematocrit levels (pA conduta para reposição volêmica em pacientes traumatizados, hemodiluídos não está bem estabelecida. Objetivo: Avaliar o efeito da hemodiluição sobre a pressão arterial média (PAM, bem como a resposta de ratos agudamente hemodiluídos e submetidos a choque hemorrágico, à reanimação com diferentes soluções. Métodos: 180 ratos foram hemodiluídos, simulando pacientes traumatizados com sangramento persistente após reposição com soluções isotônicas. Trinta ratos hemodiluídos (grupo ANE foram observados, sem tratamento. Os demais 150 foram submetidos a choque hipovolêmico e randomizados em 5 grupos segundo a opção terapêutica: CTL (controle, sem tratamento subseqüente após hipovolemia hemorrágica; SAL4 que

  18. Radiographic findings in type 3 b Gaucher disease

    Energy Technology Data Exchange (ETDEWEB)

    Hill, S.C. [Diagnostic Radiology Department, National Institutes of Health, Bethesda, MD (United States); Damaska, B.M. [Diagnostic Radiology Department, National Institutes of Health, Bethesda, MD (United States); Tsokos, M. [Laboratory of Pathology, National Institutes of Health, Bethesda, MD (United States); Kreps, C. [Developmental and Metabolic Neurology Branch, National Institutes of Health, Bethesda, MD (United States); Brady, R.O. [Developmental and Metabolic Neurology Branch, National Institutes of Health, Bethesda, MD (United States); Barton, N.W. [Developmental and Metabolic Neurology Branch, National Institutes of Health, Bethesda, MD (United States)

    1996-12-01

    The purpose of this paper is to describe the radiographic findings in type 3 b Gaucher disease, a chronic neuronopathic form of the illness with severe systemic manifestations. Between 1980 and 1985 17 consecutive patients were evaluated with radiography of the chest, long bones and spine, CT of the head and chest, abdominal sonography, and MRI of the head, abdomen and spine. Clinical manifestations were severe, and led to death from hepatic, pulmonary or cardiac failure in nine patients. Type 3 b Gaucher disease shares the same spectrum of radiographic findings observed in type 1 disease, but the systemic manifestations are more severe. Pulmonary infiltrates, thoracic lymph node enlargement, vertebral compression fractures and osteonecrosis of the long bones occur much more frequently in patients with type 3 b disease. (orig.). With 7 figs., 2 tabs.

  19. Crystal structure of inactive form of Rab3B

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Wei [Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Huazhong Normal University, Wuhan 430079 (China); Shen, Yang [Structural Genomics Consortium, University of Toronto, 101 College St., Toronto, Ontario, Canada M5G 1L7 (Canada); Jiao, Ronghong [Department of Function Inspection, Hebei Provincial People' s Hospital, Shijiazhuang 050051 (China); Liu, Yanli; Deng, Lingfu [Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Huazhong Normal University, Wuhan 430079 (China); Qi, Chao, E-mail: qichao@mail.ccnu.edu.cn [Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Huazhong Normal University, Wuhan 430079 (China)

    2012-02-24

    Highlights: Black-Right-Pointing-Pointer This is the first structural information of human Rab3B. Black-Right-Pointing-Pointer To provides a structural basis for the GDP/GTP switch in controlling the activity of Rab3. Black-Right-Pointing-Pointer The charge distribution of Rab3B indicates its unique roles in vesicular trafficking. -- Abstract: Rab proteins are the largest family of ras-related GTPases in eukaryotic cells. They act as directional molecular switches at membrane trafficking, including vesicle budding, cargo sorting, transport, tethering, and fusion. Here, we generated and crystallized the Rab3B:GDP complex. The structure of the complex was solved to 1.9 A resolution and the structural base comparison with other Rab3 members provides a structural basis for the GDP/GTP switch in controlling the activity of small GTPase. The comparison of charge distribution among the members of Rab3 also indicates their different roles in vesicular trafficking.

  20. Identification of the heparin binding site on adeno-associated virus serotype 3B (AAV-3B)

    Energy Technology Data Exchange (ETDEWEB)

    Lerch, Thomas F.; Chapman, Michael S. (Oregon HSU)

    2012-05-24

    Adeno-associated virus is a promising vector for gene therapy. In the current study, the binding site on AAV serotype 3B for the heparan sulfate proteoglycan (HSPG) receptor has been characterized. X-ray diffraction identified a disaccharide binding site at the most positively charged region on the virus surface. The contributions of basic amino acids at this and other sites were characterized using site-directed mutagenesis. Both heparin and cell binding are correlated to positive charge at the disaccharide binding site, and transduction is significantly decreased in AAV-3B vectors mutated at this site to reduce heparin binding. While the receptor attachment sites of AAV-3B and AAV-2 are both in the general vicinity of the viral spikes, the exact amino acids that participate in electrostatic interactions are distinct. Diversity in the mechanisms of cell attachment by AAV serotypes will be an important consideration for the rational design of improved gene therapy vectors.

  1. Identification of the heparin binding site on adeno-associated virus serotype 3B (AAV-3B)

    Energy Technology Data Exchange (ETDEWEB)

    Lerch, Thomas F.; Chapman, Michael S., E-mail: chapmami@ohsu.edu

    2012-02-05

    Adeno-associated virus is a promising vector for gene therapy. In the current study, the binding site on AAV serotype 3B for the heparan sulfate proteoglycan (HSPG) receptor has been characterized. X-ray diffraction identified a disaccharide binding site at the most positively charged region on the virus surface. The contributions of basic amino acids at this and other sites were characterized using site-directed mutagenesis. Both heparin and cell binding are correlated to positive charge at the disaccharide binding site, and transduction is significantly decreased in AAV-3B vectors mutated at this site to reduce heparin binding. While the receptor attachment sites of AAV-3B and AAV-2 are both in the general vicinity of the viral spikes, the exact amino acids that participate in electrostatic interactions are distinct. Diversity in the mechanisms of cell attachment by AAV serotypes will be an important consideration for the rational design of improved gene therapy vectors.

  2. Culture of Schools. Final Report. Volume IV.

    Science.gov (United States)

    American Anthropological Association, Washington, DC.

    The final volume of this 4-volume report contains further selections from "Anthropological Perspectives on Education," a monograph to be published by Basic Books of New York. (Other selections are in Vol. III, SP 003 902.) Monograph selections appearing in this volume are: "Great Tradition, Little Tradition, and Formal Education;""Indians,…

  3. 18 CFR 3b.226 - Accounting of disclosures.

    Science.gov (United States)

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Accounting of... IDENTIFIABLE PERSONAL INFORMATION Rules for Disclosure of Records § 3b.226 Accounting of disclosures. (a) The....225(b) (5) and (7). (b) Each system manager will retain the accounting made under paragraph (a)...

  4. The in-beam tracking detectors for R3B

    Energy Technology Data Exchange (ETDEWEB)

    Paschalis, Stefanos; Johansen, Jacob; Scheit, Heiko [Institut fuer Kernphysik, Technische Universitaet, D 64289 Darmstadt (Germany); Heil, Michael [GSI Helmholtzzentrum fuer Schwerionenforschung GmbH, D-64291 Darmstadt (Germany); Aumann, Thomas [Institut fuer Kernphysik, Technische Universitaet, D 64289 Darmstadt (Germany); GSI Helmholtzzentrum fuer Schwerionenforschung GmbH, D-64291 Darmstadt (Germany); Krivshich, Anatoly [PNPI St. Petersburg, 188300 Gatchina (Russian Federation); Collaboration: R3B-Collaboration

    2015-07-01

    The R3B experiment is part of the NUSTAR pillar at FAIR. One of the great strengths of the R3B experiment is the kinematically complete measurement of reactions with exotic ions with energies of up to 1 AGeV. Key components of the R3B experiment are the neutron detector NeuLAND, the γ and charge-particle calorimeter CALIFA, the Si Tracker and the in-beam tracking detectors. A cornerstone instrument of the setup is the new dipole magnet (GLAD) which bends and momentum analyses the high-rigidity beams. A precise tracking of the charged particles through the magnetic field is crucial to resolve the masses of heavy ions and measure the momentum of the fragments with high resolution. In this contribution we present the technical design details of the in-beam tracking detectors that will be used in the R3B experiment together with recent results obtained from in-beam prototype testing. In particular, we discuss Si detectors, detectors based on plastic-scintillator fibers and paddles, straw-tube gas detectors and the overall performance of the system.

  5. Design of 3B3 beamline control system

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Both structure and design of the control system in BSRF-3B3 beamline, are discussed and the subsystems are described. The LabVIEW software has advantages on data collecting and real time inspecting. In the process of data collecting, some methods are taken to solve the problems that may be met.

  6. Volúmetro com câmaras comunicantes: apresentação de um protótipo de dimensões reduzidas desenvolvido para a avaliação objetiva do volume de mão Volumeter with communicating chambers: presentation of a prototype with reduced dimensions developed for objective assessment of hand volume

    Directory of Open Access Journals (Sweden)

    Rogério Mendonça de Carvalho

    2010-06-01

    Full Text Available A volumetria tradicional por deslocamento de água, que utiliza o princípio do transbordamento de Arquimedes, é considerada o padrão-ouro da avaliação do volume de membros, porém tem seu uso rotineiro prescindido por apresentar uma série de desvantagens: o método é embaraçoso ao paciente, pouco prático e desprovido de higiene, além de consumir muito tempo do examinador. Este artigo apresenta o protótipo de um aparelho inédito que utiliza o princípio dos vasos comunicantes de Pascal para a avaliação direta do volume de mão.Traditional water displacement volumetry, based on Archimedes' principle of buoyancy, is considered the gold-standard for limb volume measurement. However, the routine use of this technique is associated with a series of disadvantages: the method is cumbersome to the patient, messy and unhygienic, and it takes a great amount of the examiner's time. This article presents the prototype of a new piece of equipment designed according to Pascal's principle of communicating vases for the direct evaluation of hand volume.

  7. Synthesis and cytotoxicity of thieno[2,3-b]pyridine and furo[2,3-b]pyridine derivatives.

    Science.gov (United States)

    Hung, Joy M; Arabshahi, Homayon J; Leung, Euphemia; Reynisson, Jóhannes; Barker, David

    2014-10-30

    Forty seven thieno[2,3-b]pyridines-2-carboxamides, furo[2,3-b]pyridines-2-carboxamides and tetrahydrothieno[2,3-b]quinolones-2-carboxamides derivatives were synthesized and tested for their antiproliferative activity against the NCI-60 cell lines. The 5-keto-tetrahydrothieno[2,3-b]quinolones-2-carboxamides (series 17) were found to have the greatest activity, with the compound containing a 3-methoxyphenylcarboxamide (compound 17d) being the most active, with GI50 values in the low nanomolar range against a range of cell lines, in particular the melanoma cell line MDA-MD-435 (GI50 - 23 nM) and the breast cancer cell line MDA-MB-468 (GI50 - 46 nM). Molecular modelling of series 17 against phosphoinositide specific-phospholipase C reveals that the side chains of the amino acids His356, Glu341, Arg549 and Lys438 are involved in hydrogen bonding with the ligands as well as a lipophilic pocket is occupied by the phenyl carboxamide moiety.

  8. Estudo radiológico da dispersão de diferentes volumes de anestésico local no bloqueio de plexo braquial pela via posterior Estudio radiológico de la dispersión de diferentes volúmenes de anestésico local en el bloqueo de plexo braquial por vía posterior Radiological evaluation of the spread of different local anesthetic volumes during posterior brachial plexus block

    Directory of Open Access Journals (Sweden)

    Marcos Guilherme Cunha Cruvinel

    2005-10-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: A dispersão do anestésico local no bloqueio interescalênico foi bem estudada, porém no bloqueio pela via posterior são poucos os estudos. O objetivo deste estudo foi determinar a dispersão de diferentes volumes de anestésico local nesta técnica através de exame radiológico contrastado. MÉTODO: Dezesseis pacientes submetidos a bloqueio do plexo braquial pela via posterior, 15 foram divididos aleatoriamente em três grupos de cinco: Grupo 1: volume de 20 mL; Grupo 2: volume de 30 mL; Grupo 3: volume de 40 mL. Em um paciente, submetido a bloqueio contínuo do plexo braquial pela via posterior, a administração de um volume de 10 mL foi estudada. Em todos, o anestésico usado foi a ropivacaína a 0,375% associada a solução radiopaca. Foram feitas radiografias da região cervical imediatamente após o bloqueio que foi avaliado através da pesquisa de sensibilidade térmica utilizando-se algodão embebido em álcool, 30 minutos após a sua realização e na sala de recuperação pós-anestésica. RESULTADOS: O comportamento radiológico e clínico do bloqueio de plexo braquial pela via posterior é muito semelhante aquele descrito com a técnica de Winnie (interescalênico. Invariavelmente há envolvimento do plexo cervical e das raízes mais altas (C5-C7 do plexo braquial. CONCLUSÕES: Este estudo mostra que a dispersão do anestésico local no bloqueio do plexo braquial pela via posterior se dá primariamente nas raízes responsáveis pela inervação do ombro.JUSTIFICATIVA Y OBJETIVOS: La dispersión del anestésico local en el bloqueo interescalénico fue bien estudiada; en el bloqueo por la vía posterior son pocos los estudios. El objetivo de este estudio, fue determinar la dispersión de diferentes volúmenes de anestésico local en esta técnica a través de examen radiológico contrastado. MÉTODO: Dieciséis pacientes sometidos a bloqueo del plexo braquial por vía posterior, 15 fueron divididos

  9. Africa Insight - Vol 38, No 3 (2008)

    African Journals Online (AJOL)

    Journal Home > Archives > Vol 38, No 3 (2008) ... The impact of sport on nation building: A Critical Analysis of South Africa and the 2010 FIFA World ... Sport tourism: comparing participant profiles and impact of three one-day events in South ...

  10. Vol 38 2010 Art 1.pub

    African Journals Online (AJOL)

    user

    ISSN 0378-5254 Journal of Family Ecology and Consumer Sciences, Vol 38, 2010. Soft drink ... area, City of Cape Town,. South Africa and the factors influencing the consumption. 1 ... gaskoeldrank deur die ouers self, die beskikbaarheid daarvan in die ...... drinks were the favourite beverage to buy at the tuck shop. In both ...

  11. Crisis Communication (Handbooks of Communication Science Vol. 23)

    DEFF Research Database (Denmark)

    Vol. 23 - The Handbook of Communication Science General editors: Peter J. Schultz and Paul Cobley......Vol. 23 - The Handbook of Communication Science General editors: Peter J. Schultz and Paul Cobley...

  12. Late Type 3b Endoleak with an Endurant Endograft

    Directory of Open Access Journals (Sweden)

    Mehmet Barburoglu

    2015-01-01

    Full Text Available Endovascular stent grafting with different commercially available stent graft systems is widely applied for the treatment of abdominal aortic aneurysms with high success rates in the current era. Various types of endoleaks are potential complications of the procedure. They usually occur in the early period. In this report, we present type 3b endoleak occurring 14 months after a successful endovascular abdominal aortic aneurysm repair with a Medtronic Endurant stent graft.

  13. Anestesia espinhal com 10 mg de bupivacaína hiperbárica associada a 5 µg de sufentanil para cesariana: estudo de diferentes volumes Anestesia espinal con 10 mg de bupivacaína hiperbárica asociada a 5 µg de sufentanil para cesárea: estudio de diferentes volúmenes Spinal Block with 10 mg of hyperbaric bupivacaine associated with 5 µg of sufentanil for cesarean section: study of different volumes

    Directory of Open Access Journals (Sweden)

    Angélica de Fátima de Assunção Braga

    2010-04-01

    alteraciones fisiológicas inherentes al embarazo, baricidad, dosis y volumen del anestésico local. El objetivo de este estudio fue evaluar la efectividad y los efectos colaterales de diferentes volúmenes de la asociación de bupivacaína hiperbárica y sufentanil por vía subaracnoidea en cesáreas. MÉTODO: Cuarenta pacientes, ASA I y II, sometidos a cesárea electiva bajo raquianestesia distribuidos en dos grupos, de acuerdo con el volumen de la solución anestésica usada: Grupo I (4 mL y Grupo II (3 mL. En los dos grupos, el anestésico local empleado fue la bupivacaína hiperbárica (10 mg-2 mL asociada al sufentanil (5 µg-1 mL. En el Grupo I, para la obtención del volumen de 4 mL, se añadió 1 mL de solución fisiológica a 0,9%. Se evaluaron: latencia del bloqueo; nivel máximo del bloqueo sensitivo; grado del bloqueo motor; tiempo para regresión del bloqueo motor; duración total de la analgesia; efectos adversos maternos y repercusiones neonatales. RESULTADOS: La latencia, el nivel máximo del bloqueo sensitivo, el grado y el tiempo para la regresión del bloqueo motor fueron similares en los dos grupos; la duración de la analgesia fue mayor en el Grupo I, con una diferencia significativa con relación al Grupo II. Los efectos adversos se dieron a menudo de forma similar en los dos grupos. Se registró la ausencia de las alteraciones cardiocirculatorias maternas y de las repercusiones neonatales. CONCLUSIONES: La bupivacaína hiperbárica en dosis de 10 mg asociada al sufentanil en dosis de 5 µg, con un volumen de 4 mL, fue más eficaz que la misma asociación en un menor volumen (3 mL, proporcionando una mejor analgesia intra y postoperatoria, sin repercusiones materno-fetales.BACKGROUND AND OBJECTIVES: Several factors affect the cephalad dispersion of the anesthetic solution in the subarachnoid space; among them, physiological changes of pregnancy and the dose and volume of the local anesthetics should be mentioned. The objective of this study was to assess

  14. The Activation of Phytophthora Effector Avr3b by Plant Cyclophilin is Required for the Nudix Hydrolase Activity of Avr3b.

    Science.gov (United States)

    Kong, Guanghui; Zhao, Yao; Jing, Maofeng; Huang, Jie; Yang, Jin; Xia, Yeqiang; Kong, Liang; Ye, Wenwu; Xiong, Qin; Qiao, Yongli; Dong, Suomeng; Ma, Wenbo; Wang, Yuanchao

    2015-08-01

    Plant pathogens secrete an arsenal of effector proteins to impair host immunity. Some effectors possess enzymatic activities that can modify their host targets. Previously, we demonstrated that a Phytophthora sojae RXLR effector Avr3b acts as a Nudix hydrolase when expressed in planta; and this enzymatic activity is required for full virulence of P. sojae strain P6497 in soybean (Glycine max). Interestingly, recombinant Avr3b produced by E. coli does not have the hydrolase activity unless it was incubated with plant protein extracts. Here, we report the activation of Avr3b by a prolyl-peptidyl isomerase (PPIase), cyclophilin, in plant cells. Avr3b directly interacts with soybean cyclophilin GmCYP1, which activates the hydrolase activity of Avr3b in a PPIase activity-dependent manner. Avr3b contains a putative Glycine-Proline (GP) motif; which is known to confer cyclophilin-binding in other protein substrates. Substitution of the Proline (P132) in the putative GP motif impaired the interaction of Avr3b with GmCYP1; as a result, the mutant Avr3bP132A can no longer be activated by GmCYP1, and is also unable to promote Phytophthora infection. Avr3b elicits hypersensitive response (HR) in soybean cultivars producing the resistance protein Rps3b, but Avr3bP132A lost its ability to trigger HR. Furthermore, silencing of GmCYP1 rendered reduced cell death triggered by Avr3b, suggesting that GmCYP1-mediated Avr3b maturation is also required for Rps3b recognition. Finally, cyclophilins of Nicotiana benthamiana can also interact with Avr3b and activate its enzymatic activity. Overall, our results demonstrate that cyclophilin is a "helper" that activates the enzymatic activity of Avr3b after it is delivered into plant cells; as such, cyclophilin is required for the avirulence and virulence functions of Avr3b.

  15. Crystal structure of inactive form of Rab3B

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Wei; Shen, Yang; Jiao, Ronghong; Liu, Yanli; Deng, Lingfu; Qi, Chao (Hebei); (Toronto); (Huazhong)

    2012-06-28

    Rab proteins are the largest family of ras-related GTPases in eukaryotic cells. They act as directional molecular switches at membrane trafficking, including vesicle budding, cargo sorting, transport, tethering, and fusion. Here, we generated and crystallized the Rab3B:GDP complex. The structure of the complex was solved to 1.9 {angstrom} resolution and the structural base comparison with other Rab3 members provides a structural basis for the GDP/GTP switch in controlling the activity of small GTPase. The comparison of charge distribution among the members of Rab3 also indicates their different roles in vesicular trafficking.

  16. 3B1 beam line and its physical properties

    Science.gov (United States)

    Yi, Futing; Wang, Peiwei; Jin, Ming; Wu, Jianwu

    1995-02-01

    The 3B1 beam line is in the hall of 13# on BSRF, and is used for the lithography, biology and multilayer reflection research. On the line, there are a cylinder mirror system, a vacuum control system, an exposure chamber and a beryllium window for lithography research. The mirror coated with gold receives the synchrotron radiation at a glance angle of 1.5°, and scans in the vertical for lithography. The light, reflected by the scanning mirror and absorbed by the window, produces a spectrum of 4-20 Å and a uniformity spot for the lithography.

  17. Determinação de volumes e pressões de balonetes de tubos traqueais insuflados com ar ambiente ou óxido nitroso Determinación de volúmenes y presiones de balones de tubos traqueales insuflados con aire ambiente u óxido nitroso Volume and pressure of tracheal tube cuffs filled with air or nitrous oxide

    Directory of Open Access Journals (Sweden)

    Ernesto Leonardo Cárpio Peña

    2004-06-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: A pressão exercida pelo balonete do tubo traqueal contra a parede da traquéia deve permitir fluxo capilar adequado e prevenir escapes de ar ou aspiração pulmonar. Esta pesquisa procurou determinar as variações de pressão do balonete insuflado com ar ambiente ou com óxido nitroso a 100%. MÉTODO: Trinta pacientes foram selecionados para receber anestesia geral balanceada com intubação orotraqueal. O balonete foi insuflado conforme critérios clínicos. As medidas de base foram realizadas após 15 minutos do início da anestesia com um manômetro aneróide calibrado em cm de H2O e forneceram os valores iniciais de pressão e volume. Os pacientes foram divididos em dois grupos: balonete reinsuflado com ar ambiente, grupo A, ou com óxido nitroso, grupo B. As medidas de pressão foram obtidas em intervalos até a primeira hora e os resultados comparados. RESULTADOS: Os grupos mostraram-se comparáveis para idade e sexo. Em ambos os grupos os valores basais médios para pressão foram próximos de 40 cmH2O com 8 ml de volume. No grupo com ar ambiente, as pressões aumentaram até 36 cmH2O em uma hora. No grupo de balonete insuflado com N2O, as pressões diminuíram abaixo de 20 cmH2O entre 20 e 30 minutos de anestesia. CONCLUSÕES: O uso de N2O a 100% para insuflação do balonete de sonda traqueal não constitui método seguro, acarretando progressiva perda da capacidade de vedação. O uso de ar ambiente promove aumento de volume e de pressão no balonete, aumentando possibilidade de lesão da mucosa traqueal.JUSTIFICATIVA Y OBJETIVOS: La presión ejercida por el balón del tubo traqueal contra la pared de la traquea debe permitir flujo capilar adecuado y prevenir escapes de aire o aspiración pulmonar. Esta pesquisa buscó determinar las variaciones de presión del balón insuflado con aire ambiente o con óxido nitroso a 100%. MÉTODO: Treinta pacientes fueron seleccionados para recibir anestesia general

  18. El rol del lifting endoscópico fronto-témporo-orbitario en el nuevo concepto de rejuvenecimiento facial: Mini-invasivo, tensión moderada, restauración de volúmenes The importance of brow-temporal-orbital endoscopic facelift in the new concept of facial rejuvenation: low invasiveness, moderate tension, volume restauration

    Directory of Open Access Journals (Sweden)

    A. Gennai

    2009-03-01

    Full Text Available En el concepto moderno de rejuvenecimiento facial, lo principal es conseguir un resultado natural evitando las marcas quirúrgicas y limitando las cicatrices. El lifting endoscopio fronto-témporo-orbitario es, en nuestra opinión, el mejor método para conseguir este objetivo. Entre sus peculiaridades están el ser una técnica mínimamente invasiva, que proporciona una tensión moderada natural a los tejidos blandos de la cara y redistribuye y restaura los volúmenes faciales con el mínimo de resección cutánea, logrando un resultado lo más natural posible. Al mismo tiempo, conseguimos la armonía entre las estructuras anatómicas faciales, mínimas incisiones y mínimas cicatrices. En nuestra práctica, cuando es necesario, asociamos lipofilling del área zigomático-malar, de la región perioral y de las mejillas y un rejuvenecimiento facial superficial con láser de Erbio. Para el tercio inferior de la cara empleamos estiramiento cervicofacial con cicatriz reducida. Entre Noviembre de 1998 y Diciembre de 2006 hemos realizado 221 liftings endoscópicos fronto-témporo-orbitarios: 21 hombres y 200 mujeres de edades comprendidas entre los 28 y los 55 años. Practicamos la disección en el plano infra o supragaleal según las zonas; continuamos seccionando el músculo procerus, el corrugador y la porción orbitaria del músculo orbicular a nivel del párpado inferior. Finalmente, practicamos una tracción sagital fijando puntos de acuerdo a un vector paramediano y a otro horario. Los resultados a largo plazo (6, 12 y 24 meses revelan una notable satisfacción por parte de los pacientes con un resultado estético que permanece natural en el tiempo.Primary object in modern concept of facial rejuvenation is to give a natural result, reducing the surgical cuts and limiting the scars. The fronto-temporal-orbital endoscopic facelift is, in our opinion, the best way to satisfy this purpose. Its peculiarities, such as mini-invasive technique

  19. Culture of Schools. Final Report. Volume III.

    Science.gov (United States)

    American Anthropological Association, Washington, DC.

    The third volume of this 4-volume report contains the last two speeches, on educational philosophy and the role of reason in society, from the Colloquium on the Culture of Schools held at the New School for Social Research (preceding speeches are in Vol. II, SP 003 901), reports on conferences on the culture of schools held in Pittsburgh and…

  20. Role of the DNA methyltransferase variant DNMT3b3 in DNA methylation.

    Science.gov (United States)

    Weisenberger, Daniel J; Velicescu, Mihaela; Cheng, Jonathan C; Gonzales, Felicidad A; Liang, Gangning; Jones, Peter A

    2004-01-01

    Several alternatively spliced variants of DNA methyltransferase (DNMT) 3b have been described. Here, we identified new murine Dnmt3b mRNA isoforms and found that mouse embryonic stem (ES) cells expressed only Dnmt3b transcripts that contained exons 10 and 11, whereas the Dnmt3b transcripts in somatic cells lacked these exons, suggesting that this region is important for embryonic development. DNMT3b2 and 3b3 were the major isoforms expressed in human cell lines and the mRNA levels of these isoforms closely correlated with their protein levels. Although DNMT3b3 may be catalytically inactive, it still may be biologically important because D4Z4 and satellites 2 and 3 repeat sequences, all known DNMT3b target sequences, were methylated in cells that predominantly expressed DNMT3b3. Treatment of cells with the mechanism-based inhibitor 5-aza-2'-deoxycytidine (5-Aza-CdR) caused a complete depletion of DNMT1, 3a, 3b1, and 3b2 proteins. Human DNMT3b3 and the murine Dnmt3b3-like isoform, Dnmt3b6, were also depleted although less efficiently, suggesting that DNMT3b3 also may be capable of DNA binding. Moreover, de novo methylation of D4Z4 in T24 cancer cells after 5-Aza-CdR treatment only occurred when DNMT3b3 was expressed, reinforcing its role as a contributing factor of DNA methylation. The expression of either DNMT3b2 or 3b3, however, was not sufficient to explain the abnormal methylation of DNMT3b target sequences in human cancers, which may therefore be dependent on factors that affect DNMT3b targeting. Methylation analyses of immunodeficiency, chromosomal instabilities, and facial abnormalities cells revealed that an Alu repeat sequence was highly methylated, suggesting that Alu sequences are not DNMT3b targets.

  1. Role of DNMT3B in the regulation of early neural and neural crest specifiers

    OpenAIRE

    Martins-Taylor, Kristen; Schroeder, Diane I.; LaSalle, Janine M.; Lalande, Marc; Xu, Ren-He

    2012-01-01

    The de novo DNA methyltransferase DNMT3B functions in establishing DNA methylation patterns during development. DNMT3B missense mutations cause immunodeficiency, centromere instability and facial anomalies (ICF) syndrome. The restriction of Dnmt3b expression to neural progenitor cells, as well as the mild cognitive defects observed in ICF patients, suggests that DNMT3B may play an important role in early neurogenesis. We performed RNAi knockdown of DNMT3B in human embryonic stem cells (hESCs)...

  2. El espectro de K2 Vol

    Science.gov (United States)

    Pintado, O. I.; Adelman, S. J.

    Se han obtenido espectros de alta dispersión para la estrella K2 Vol con el espectrógrafo REOSC del CASLEO en un rango de longitudes de ondas comprendido entre 3500 y 5050 Å. El espectro muestra evidencias de que la estrella es binaria. Se determinan las abundancias de los componentes químicos presentes en su atmósfera, como así también algunas características de su compañera.

  3. Efeitos da administração subaracnóidea de grandes volumes de lidocaína a 2% e ropivacaína a 1% sobre a medula espinhal e as meninges: estudo experimental em cães Efectos de la administración subaracnóidea de grandes volúmenes de lidocaína a 2% y ropivacaína a 1% sobre la médula espinal y las meninges: estudio experimental en perros Effects of spinal administration of large volumes of 2% lidocaine and 1% ropivacaine on spinal cord and meninges: experimental study in dogs

    Directory of Open Access Journals (Sweden)

    Eliana Marisa Ganem

    2003-06-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: A injeção de grandes volumes de anestésico local no espaço subaracnóideo, após punção dural acidental, é complicação da anestesia peridural. O objetivo desta pesquisa foi investigar as possíveis alterações clínicas e histológicas desencadeadas por grandes volumes de lidocaína a 2% e ropivacaína a 1%, simulando injeção subaracnóidea acidental, em cães. MÉTODO: Vinte e um cães foram distribuídos aleatoriamente em 3 grupos, que receberam por via subaracnóidea: G1 - cloreto de sódio a 0,9%; G2 - lidocaína a 2% e G3 - ropivacaína a 1%. A punção subaracnóidea foi realizada no espaço intervertebral L6-L7. O volume de anestésico local administrado foi de 1 ml para cada 10 cm de distância entre a protuberância occipital e o espaço lombossacral (5 - 6,6 ml. Após 72 horas de observação clínica os animais foram sacrificados e foi removida a porção lombossacral da medula para exame histológico, por microscopia óptica. RESULTADOS: Nenhum animal do G1 apresentou alterações clínicas ou histológicas da medula espinhal. Foram observados dois casos de necrose do tecido nervoso em G2, porém mudanças clínicas, em somente um desses cães e em outros dois animais que não apresentaram alterações histológicas. Foi encontrada necrose focal do tecido nervoso medular em um animal de G3. Todos os animais de G3 permaneceram clinicamente normais. CONCLUSÕES: Conclui-se que grandes volumes de lidocaína a 2% determinaram alterações clínicas e histológicas mais intensas que os de ropivacaína a 1%.JUSTIFICATIVA Y OBJETIVOS: La inyección de grandes volúmenes de anestésico local en el espacio subaracnóideo, después de punción dural accidental, es complicación de la anestesia peridural. El objetivo de esta pesquisa fue investigar las posibles alteraciones clínicas e histológicas desencadenadas por grandes volúmenes de lidocaína a 2% y ropivacaína a 1%, simulando una inyecci

  4. [SMEAC Newsletters, Science Education, Vol. 1, No. 1--Vol. 2, No. 1, 1967-1968].

    Science.gov (United States)

    ERIC Clearinghouse for Science, Mathematics, and Environmental Education, Columbus, OH.

    Each of these newsletters, produced by the ERIC Information Analysis Center for Science, Mathematics, and Environmental Education, contains information concerning center publications and other items considered of interest to researchers and educators of various education levels. Vol. 1, No. 1 highlights selected bibliographies (no longer produced…

  5. [SMEAC Newsletters, Science Education, Vol. 2, No. 2--Vol. 2, No. 3, 1969].

    Science.gov (United States)

    ERIC Clearinghouse for Science, Mathematics, and Environmental Education, Columbus, OH.

    Each of these newsletters, produced by the ERIC Information Analysis Center for Science, Mathematics, and Environmental Education, Contains information concerning center publications and activities, as well as other items considered of interest to researchers and educators of various educational levels. One of the emphases in Vol. 2, No. 2, is a…

  6. Modulation of Dnmt3b function in vitro by interactions with Dnmt3L, Dnmt3a and Dnmt3b splice variants.

    Science.gov (United States)

    Van Emburgh, Beth O; Robertson, Keith D

    2011-07-01

    DNA methylation, an essential regulator of transcription and chromatin structure, is established and maintained by the coordinated action of three DNA methyltransferases: DNMT1, DNMT3A and DNMT3B, and the inactive accessory factor DNMT3L. Disruptions in DNMT3B function are linked to carcinogenesis and genetic disease. DNMT3B is also highly alternatively spliced in a tissue- and disease-specific manner. The impact of intra-DNMT3 interactions and alternative splicing on the function of DNMT3 family members remains unclear. In the present work, we focused on DNMT3B. Using a panel of in vitro assays, we examined the consequences of DNMT3B splicing and mutations on its ability to bind DNA, interact with itself and other DNMT3's, and methylate DNA. Our results show that, while the C-terminal catalytic domain is critical for most DNMT3B functions, parts of the N-terminal region, including the PWWP domain, are also important. Alternative splicing and domain deletions also influence DNMT3B's cellular localization. Furthermore, our data reveal the existence of extensive DNMT3B self-interactions that differentially impact on its activity. Finally, we show that catalytically inactive isoforms of DNMT3B are capable of modulating the activity of DNMT3A-DNMT3L complexes. Our studies therefore suggest that seemingly 'inactive' DNMT3B isoforms may influence genomic methylation patterns in vivo.

  7. ∆DNMT3B4-del Contributes to Aberrant DNA Methylation Patterns in Lung Tumorigenesis

    Directory of Open Access Journals (Sweden)

    Mark Z. Ma

    2015-10-01

    Full Text Available Aberrant DNA methylation is a hallmark of cancer but mechanisms contributing to the abnormality remain elusive. We have previously shown that ∆DNMT3B is the predominantly expressed form of DNMT3B. In this study, we found that most of the lung cancer cell lines tested predominantly expressed DNMT3B isoforms without exons 21, 22 or both 21 and 22 (a region corresponding to the enzymatic domain of DNMT3B termed DNMT3B/∆DNMT3B-del. In normal bronchial epithelial cells, DNMT3B/ΔDNMT3B and DNMT3B/∆DNMT3B-del displayed equal levels of expression. In contrast, in patients with non-small cell lung cancer NSCLC, 111 (93% of the 119 tumors predominantly expressed DNMT3B/ΔDNMT3B-del, including 47 (39% tumors with no detectable DNMT3B/∆DNMT3B. Using a transgenic mouse model, we further demonstrated the biological impact of ∆DNMT3B4-del, the ∆DNMT3B-del isoform most abundantly expressed in NSCLC, in global DNA methylation patterns and lung tumorigenesis. Expression of ∆DNMT3B4-del in the mouse lungs resulted in an increased global DNA hypomethylation, focal DNA hypermethylation, epithelial hyperplastia and tumor formation when challenged with a tobacco carcinogen. Our results demonstrate ∆DNMT3B4-del as a critical factor in developing aberrant DNA methylation patterns during lung tumorigenesis and suggest that ∆DNMT3B4-del may be a target for lung cancer prevention.

  8. Assessment of RAMONA-3B methodology with FRIGG dynamic tests

    Energy Technology Data Exchange (ETDEWEB)

    Rohatgi, U.S.; Neymotin, L.Y.; Wulff, W.

    1990-12-31

    The computer codes used at Brookhaven National Laboratory to compute BWR safety parameters are the Engineering Plant Analyzer (EPA) and RAMONA-3B/MOD1. Both codes have the same methodology for modeling thermal hydraulic phenomena: drift-flux formulation, two-phase multipliers for the wall friction and form losses calculations, and the momentum integral approach for spatial integration of the loop momentum equations. Both codes use explicit integration methods for solving ordinary differential equations. It is concluded that both the codes are capable of modelling the instability problems for a BWR. The accuracy of thermohydraulics codes predictions was assessed by modelling oscillatory FRIGG tests. Nodalizations studies showed that 24 axial nodes were sufficient for a converged solution, 12 axial nodes produced an error of 4.4% in the gain of the power to flow transfer function. The code predicted consistently the effects of power and inlet subcooling on gain and system resonance frequency. The comparisons showed that the code predicted the peak gains with a mean difference from experiments of 7% {plus_minus} 30% for all the tests modeled. The uncertainty in the experimental data is {minus}11% to +12%. The mean difference in the predicted frequency at the peak gain is {minus}6% {plus_minus} 14%.

  9. Assessment of RAMONA-3B methodology with FRIGG dynamic tests

    Energy Technology Data Exchange (ETDEWEB)

    Rohatgi, U.S.; Neymotin, L.Y.; Wulff, W.

    1990-01-01

    The computer codes used at Brookhaven National Laboratory to compute BWR safety parameters are the Engineering Plant Analyzer (EPA) and RAMONA-3B/MOD1. Both codes have the same methodology for modeling thermal hydraulic phenomena: drift-flux formulation, two-phase multipliers for the wall friction and form losses calculations, and the momentum integral approach for spatial integration of the loop momentum equations. Both codes use explicit integration methods for solving ordinary differential equations. It is concluded that both the codes are capable of modelling the instability problems for a BWR. The accuracy of thermohydraulics codes predictions was assessed by modelling oscillatory FRIGG tests. Nodalizations studies showed that 24 axial nodes were sufficient for a converged solution, 12 axial nodes produced an error of 4.4% in the gain of the power to flow transfer function. The code predicted consistently the effects of power and inlet subcooling on gain and system resonance frequency. The comparisons showed that the code predicted the peak gains with a mean difference from experiments of 7% {plus minus} 30% for all the tests modeled. The uncertainty in the experimental data is {minus}11% to +12%. The mean difference in the predicted frequency at the peak gain is {minus}6% {plus minus} 14%.

  10. An elementary proof of MinVol(Rn = 0 for n > 3

    Directory of Open Access Journals (Sweden)

    Jiaqiang Mei

    2008-12-01

    Full Text Available In this paper, we give an elementary proof of the result that the minimal volumes of R³ and R4 are zero. The approach is to construct a sequence of explicit complete metrics on them such that the sectional curvatures are bounded in absolute value by 1 and the volumes tend to zero. As a direct consequence, we get that MinVol (Rn = 0 for n > 3.Neste artigo fornecemos uma demonstração elementar do resultado de que os volumes minimais de R³ e R4 são ambos iguais a zero. A abordagem consiste na construção de uma seqüência de métricas completas explícitas nesses espaços cujas curvaturas seccionais são limitadas em valor absoluto por 1 e os volumes tendem a zero. Como conseqüência direta, estabelecemos que MinVol(Rn = 0 para n > 3.

  11. ELISA for evaluating the incorporation of plasma derived complement split-products C3b/iC3b into solid-phase immune complexes

    DEFF Research Database (Denmark)

    Zimmermann-Nielsen, E; Svehag, S E; Thorlacius-Ussing, O;

    2001-01-01

    An ELISA that measures plasma derived complement (C) split-products C3b/iC3b deposited on solid-phase immune complexes during C activation is described. Plates are coated with BSA, anti-BSA and plasma is added. Deposited C3b/iC3b is then detected by biotinylated anti-C3c-antibodies, avidin......) or classical pathway (CP) with regard to age or gender was demonstrated. The total coefficient of variation was systemic lupus erythematosus (SLE). There was a weak correlation between...

  12. Fingersomatotopy in area 3b: an fMRI-study

    Directory of Open Access Journals (Sweden)

    Rosén Birgitta

    2004-08-01

    Full Text Available Abstract Background The primary sensory cortex (S1 in the postcentral gyrus is comprised of four areas that each contain a body map, where the representation of the hand is located with the thumb most laterally, anteriorly and inferiorly and the little finger most medially, posteriorly and superiorly. Previous studies on somatotopy using functional MRI have either used low field strength, have included a small number of subjects or failed to attribute activations to any area within S1. In the present study we included twenty subjects, who were investigated at 3 Tesla (T. We focused specifically on Brodmann area 3b, which neurons have discrete receptive fields with a potentially more clearcut somatotopic organisation. The spatial distribution for all fingers' peak activation was determined and group as well as individual analysis was performed. Results Activation maps from 18 subjects were of adequate quality; in 17 subjects activations were present for all fingers and these data were further analysed. In the group analysis the thumb was located most laterally, anteriorly and inferiorly with the other fingers sequentially positioned more medially, posteriorly and superiorly. At the individual level this somatotopic relationship was present for the thumb and little finger, with a higher variability for the fingers in between. The Euclidian distance between the first and fifth finger was 17.2 mm, between the first and second finger 10.6 mm and between the remaining fingers on average 6.3 mm. Conclusion Results from the group analysis, that is both the location of the fingers and the Euclidian distances, are well comparable to results from previous studies using a wide range of modalities. On the subject level the spatial localisation of the fingers showed a less stringent somatotopic order so that the location of a finger in a single subject cannot be predicted from the group result.

  13. 'tomo_display' and 'vol_tools': IDL VM Packages for Tomography Data Reconstruction, Processing, and Visualization

    Science.gov (United States)

    Rivers, M. L.; Gualda, G. A.

    2009-05-01

    One of the challenges in tomography is the availability of suitable software for image processing and analysis in 3D. We present here 'tomo_display' and 'vol_tools', two packages created in IDL that enable reconstruction, processing, and visualization of tomographic data. They complement in many ways the capabilities offered by Blob3D (Ketcham 2005 - Geosphere, 1: 32-41, DOI: 10.1130/GES00001.1) and, in combination, allow users without programming knowledge to perform all steps necessary to obtain qualitative and quantitative information using tomographic data. The package 'tomo_display' was created and is maintained by Mark Rivers. It allows the user to: (1) preprocess and reconstruct parallel beam tomographic data, including removal of anomalous pixels, ring artifact reduction, and automated determination of the rotation center, (2) visualization of both raw and reconstructed data, either as individual frames, or as a series of sequential frames. The package 'vol_tools' consists of a series of small programs created and maintained by Guilherme Gualda to perform specific tasks not included in other packages. Existing modules include simple tools for cropping volumes, generating histograms of intensity, sample volume measurement (useful for porous samples like pumice), and computation of volume differences (for differential absorption tomography). The module 'vol_animate' can be used to generate 3D animations using rendered isosurfaces around objects. Both packages use the same NetCDF format '.volume' files created using code written by Mark Rivers. Currently, only 16-bit integer volumes are created and read by the packages, but floating point and 8-bit data can easily be stored in the NetCDF format as well. A simple GUI to convert sequences of tiffs into '.volume' files is available within 'vol_tools'. Both 'tomo_display' and 'vol_tools' include options to (1) generate onscreen output that allows for dynamic visualization in 3D, (2) save sequences of tiffs to disk

  14. Overexpression of a splice variant of DNA methyltransferase 3b, DNMT3b4, associated with DNA hypomethylation on pericentromeric satellite regions during human hepatocarcinogenesis.

    Science.gov (United States)

    Saito, Yoshimasa; Kanai, Yae; Sakamoto, Michiie; Saito, Hidetsugu; Ishii, Hiromasa; Hirohashi, Setsuo

    2002-07-23

    DNA hypomethylation on pericentromeric satellite regions is an early and frequent event associated with heterochromatin instability during human hepatocarcinogenesis. A DNA methyltransferase, DNMT3b, is required for methylation on pericentromeric satellite regions during mouse development. To clarify the molecular mechanism underlying DNA hypomethylation on pericentromeric satellite regions during human hepatocarcinogenesis, we examined mutations of the DNMT3b gene and mRNA expression levels of splice variants of DNMT3b in noncancerous liver tissues showing chronic hepatitis and cirrhosis, which are considered to be precancerous conditions, and in hepatocellular carcinomas (HCCs). Mutation of the DNMT3b gene was not found in HCCs. Overexpression of DNMT3b4, a splice variant of DNMT3b lacking conserved methyltransferase motifs IX and X, significantly correlated with DNA hypomethylation on pericentromeric satellite regions in precancerous conditions and HCCs (P = 0.0001). In particular, the ratio of expression of DNMT3b4 to that of DNMT3b3, which is the major splice variant in normal liver tissues and retains conserved methyltransferase motifs I, IV, VI, IX, and X, showed significant correlation with DNA hypomethylation (P = 0.009). Transfection of human epithelial 293 cells with DNMT3b4 cDNA induced DNA demethylation on satellite 2 in pericentromeric heterochromatin DNA. These results suggest that overexpression of DNMT3b4, which may lack DNA methyltransferase activity and compete with DNMT3b3 for targeting to pericentromeric satellite regions, results in DNA hypomethylation on these regions, even in precancerous stages, and plays a critical role in human hepatocarcinogenesis by inducing chromosomal instability.

  15. Data of evolutionary structure change: 1ADIB-1DJ3B [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1ADIB-1DJ3B 1ADI 1DJ3 B B -------GNNVVVLGTQWGDEGKGKIVDLLTERAKYVVR...KVARRGLRVGDLFDKETFAEKLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDFVMFEGAQGTLLDIDHGTYPYVTSSNTTAGG...ss_2> 0 1ADI B 1ADIB TLVIN-GEKTV... 1ADI B 1ADIB

  16. Extended Reconstructed Sea Surface Temperature (ERSST) Monthly Analysis, Version 3b

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Version 3b (v3b) of the Extended Reconstructed Sea Surface Temperature (ERSST) dataset is a monthly SST analysis on a 2-degree global grid based on the International...

  17. Expression pattern and clinical significance of DNA methyltransferase 3B variants in gastric carcinoma.

    Science.gov (United States)

    Su, Xianwei; Lv, Chengyu; Qiao, Fengchang; Qiu, Xuemei; Huang, Wenbin; Wu, Qingxiang; Zhao, Zhujiang; Fan, Hong

    2010-03-01

    The aim of this study was to detect the expression pattern of DNA methyltransferase 3B (DNMT3B) variants in primary gastric cancer (GC) and to explore the clinical significance of DNMT3B variants in gastric carcinogenesis. Specific polymerase chain reaction (PCR) primer sets were designed to distinguish individual DNMT3B variants according to their splicing patterns. Expression levels of DNMT3B variants were assessed by quantitative real-time RT-PCR in gastric cancer tissue, normal gastric mucosae and GC cell lines. The relationship between the expression patterns of the DNMT3B variants and corresponding clinical information was analyzed by observing the expression levels of different variants in the tumors. These results demonstrate that DNMT3B overexpression is related to late phase invasion (P=0.029) and intestinal type (P=0.012) in GC. DNMT3B3 expression was higher in normal tissue, compared to tumor tissue (P=0.033). In contrast, only 18, 32 and 35% of the patient tumors overexpressed DNMT3B1, DNMT3B4 and DNMT3B5, respectively. While taking into account environmental factors (H. pylori, Epstein-Barr virus infection), H. pylori infection elevated DNMT3B1 and DNMT3B3 variants in tumors, while increasing DNMT3B4 in both tumor and non-cancerous tissues. Our findings indicated that the expression of DNMT3B3 is the major splice variant in normal gastric mucosae and may be affected by H. pylori infection. Elevated DNMT3B variants may influence the progression of gastric cancer and may possibly be a powerful indicator for the disease.

  18. TMPA Products 3B42RT & 3B42V6: Evaluation and Application in Qinghai-Tibet Plateau

    Science.gov (United States)

    Hao, Z.; Sun, L.; Wang, J.

    2012-04-01

    Hydrological researchers in Qinghai-Tibet Plateau tend to be haunted by deficiency of station gauged precipitation data for the sparse and uneven distribution of local meteorological stations. Fortunately, alternative data can be obtained from TRMM (Tropic Rainfall Measurement Mission) satellite. Preliminary evaluation and necessary correction of TRMM satellite rainfall products is required for the sake of reliability and suitability considering that TRMM precipitation is unconventional and natural condition in Qinghai-Tibet Plateau is unusually complicated. 3B42RT and 3B42V6 products from TRMM Multisatellite Precipitation Analysis(TMPA) are evaluated in northeast Qinghai-Tibet Plateau with 50 stations quality-controlled gauged daily precipitation as the benchmark precipitation set. It is found that the RT data overestimates the actual precipitation greatly while V6 only overestimates it slightly. RT data shows different seasonal and inter-annual accuracies. Summer and autumn see better accuracies than winter and spring and wet years see higher accuracies than dry years. Latitude is believed to be an important factor that influences the accuracy of satellite precipitation. Both RT and V6 can reflect the general pattern of the spatial distribution of precipitation even though RT overestimates the quantity greatly. A new parameter, accumulated precipitation weight point (APWP), was introduced to describe the temporal-spatial pattern evolution of precipitation. The APWP of both RT and V6 were moving from south to north in the past decade, but they are all in the west of station gauged precipitation APWP(s).V6 APWP track fit gauged precipitation perfectly while RT APWP track has over-exaggerated legs, indicating that spatial distribution of RT precipitation experienced unreasonable sharp changes. A practical and operational procedure to correct satellite precipitation data is developed. For RT, there are two steps. Step 1, the downscaling, original daily precipitation

  19. Association of low-affinity FC gamma receptor 3B (FCGR3B) copy number variation with rheumatoid arthritis in Caucasian subjects

    NARCIS (Netherlands)

    Merriman, T.R.; Fanciulli, M.; Merriman, M.E.; Alizadeh, B.Z.; Koeleman, B.P.C.; Dalbeth, N.; Gow, P.; Harrison, A.A.; Highton, J.; Jones, P.B.; Stamp, L.K.; Steer, S.; Barrera, P.; Coenen, M.J.H.; Franke, B.; Vyse, T.; Aitman, T.; Radstake, T.; McKinney, C.

    2009-01-01

    Aim: There is increasing evidence that gene copy-number variation influences phenotypic variation. The low-affinity Fc receptor 3B (FCGR3B) is a copy-number polymorphic gene involved in the recruitment to sites of inflammation and activation of polymorphonuclear neutrophils (PMN). Given the importan

  20. Elevated APOBEC3B Correlates with Poor Outcomes for Estrogen-Receptor-Positive Breast Cancers

    NARCIS (Netherlands)

    A.M. Sieuwerts (Anieta); S. Willis (Scooter); M.J. Burns (Michael); M.P. Look (Maxime); M.E.M.-V. Gelder (Marion E. Meijer-Van); A. Schlicker (Andreas); D.A.M. Heideman (Danielle); H. Jacobs (Heinz); L. Wessels (Lodewyk); B. Leyland-Jones (Brian); K.P. Gray (Kathryn); J.A. Foekens (John); R.S. Harris (Reuben); J.W.M. Martens (John)

    2014-01-01

    textabstractRecent observations connected DNA cytosine deaminase APOBEC3B to the genetic evolution of breast cancer. We addressed whether APOBEC3B is associated with breast cancer clinical outcomes. APOBEC3B messenger RNA (mRNA) levels were related in 1,491 primary breast cancers to disease-free (DF

  1. 21 CFR 866.5260 - Complement C3b inactivator immunological test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Complement C3b inactivator immunological test... Systems § 866.5260 Complement C3b inactivator immunological test system. (a) Identification. A complement... immunochemical techniques the complement C3b inactivator (a plasma protein) in serum. Complement is a group...

  2. File list: Oth.Myo.20.Sin3b.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Myo.20.Sin3b.AllCell mm9 TFs and others Sin3b Muscle SRX022857,SRX022858,SRX022...860,SRX022859,SRX022856,SRX022855,SRX022861 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.Myo.20.Sin3b.AllCell.bed ...

  3. File list: Oth.Myo.50.Sin3b.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Myo.50.Sin3b.AllCell mm9 TFs and others Sin3b Muscle SRX022858,SRX022860,SRX022...859,SRX022856,SRX022855,SRX022857,SRX022861 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.Myo.50.Sin3b.AllCell.bed ...

  4. 18 CFR 3b.204 - Safeguarding information in manual and computer-based record systems.

    Science.gov (United States)

    2010-04-01

    ... information in manual and computer-based record systems. 3b.204 Section 3b.204 Conservation of Power and Water... Collection of Records § 3b.204 Safeguarding information in manual and computer-based record systems. (a) The administrative and physical controls to protect the information in the manual and computer-based record...

  5. File list: Oth.ALL.10.Sin3b.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.ALL.10.Sin3b.AllCell mm9 TFs and others Sin3b All cell types SRX022856,SRX02285...8,SRX022857,SRX022859,SRX022860,SRX022861,SRX022855 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.ALL.10.Sin3b.AllCell.bed ...

  6. LETTER TO THE EDITOR: Unique Fermi surfaces with quasi-one-dimensional character in CeRh3B2 and LaRh3B2

    Science.gov (United States)

    Okubo, T.; Yamada, M.; Thamizhavel, A.; Kirita, S.; Inada, Y.; Settai, R.; Harima, H.; Takegahara, K.; Galatanu, A.; Yamamoto, E.; Onuki, Y.

    2003-11-01

    We have carried out de Haas-van Alphen (dHvA) experiments on a ferromagnet CeRh3B2 with an extremely high Curie temperature T_{\\mathrm {C}} \\simeq 120 K and a non-4f reference compound LaRh3B2. The dHvA data of LaRh3B2 are well explained by the results of energy band calculations. The topology of the Fermi surfaces in CeRh3B2 is found to be very similar to that of LaRh3B2, possessing wavy but flat Fermi surfaces in the basal plane. Observation of a quasi-one-dimensional electronic state is the first such case in a rare earth compound.

  7. Modern Persian: Intermediate Level, Vol. 2.

    Science.gov (United States)

    Windfuhr, Gernot; And Others

    The second of three volumes of an intergrated course in intermediate Persian is presented. This volume encompasses material appropriate for students entering the second year of Persian studies who have strong preparation in elementary Persian. Verbal skills should be on a level which will allow comprehensive discussion of a topic using simple,…

  8. Minicourses in Astrophysics, Modular Approach, Vol. I.

    Science.gov (United States)

    Illinois Univ., Chicago.

    This is the first volume of a two-volume minicourse in astrophysics. It contains chapters on the following topics: planetary atmospheres; X-ray astronomy; radio astrophysics; molecular astrophysics; and gamma-ray astrophysics. Each chapter gives much technical discussion, mathematical treatment, diagrams, and examples. References are included with…

  9. Inactive DNMT3B Splice Variants Modulate De Novo DNA Methylation

    OpenAIRE

    Gordon, Catherine A.; Hartono, Stella R; Frédéric Chédin

    2013-01-01

    Inactive DNA methyltransferase (DNMT) 3B splice isoforms are associated with changes in DNA methylation, yet the mechanisms by which they act remain largely unknown. Using biochemical and cell culture assays, we show here that the inactive DNMT3B3 and DNMT3B4 isoforms bind to and regulate the activity of catalytically competent DNMT3A or DNMT3B molecules. DNMT3B3 modestly stimulated the de novo methylation activity of DNMT3A and also counteracted the stimulatory effects of DNMT3L, therefore l...

  10. Aldehyde dehydrogenase 3B1 (ALDH3B1): immunohistochemical tissue distribution and cellular-specific localization in normal and cancerous human tissues.

    Science.gov (United States)

    Marchitti, Satori A; Orlicky, David J; Brocker, Chad; Vasiliou, Vasilis

    2010-09-01

    Aldehyde dehydrogenase (ALDH) enzymes are critical in the detoxification of endogenous and exogenous aldehydes. Our previous findings indicate that the ALDH3B1 enzyme is expressed in several mouse tissues and is catalytically active toward aldehydes derived from lipid peroxidation, suggesting a potential role against oxidative stress. The aim of this study was to elucidate by immunohistochemistry the tissue, cellular, and subcellular distribution of ALDH3B1 in normal human tissues and in tumors of human lung, colon, breast, and ovary. Our results indicate that ALDH3B1 is expressed in a tissue-specific manner and in a limited number of cell types, including hepatocytes, proximal convoluted tubule cells, cerebellar astrocytes, bronchiole ciliated cells, testis efferent ductule ciliated cells, and histiocytes. ALDH3B1 expression was upregulated in a high percentage of human tumors (lung > breast = ovarian > colon). Increased ALDH3B1 expression in tumor cells may confer a growth advantage or be the result of an induction mechanism mediated by increased oxidative stress. Subcellular localization of ALDH3B1 was predominantly cytosolic in tissues, with the exception of normal human lung and testis, in which localization appeared membrane-bound or membrane-associated. The specificity of ALDH3B1 distribution may prove to be directly related to the functional role of this enzyme in human tissues.

  11. Magnetic and Transport Studies in LaxGd1-xRh3B2 and CexGd1-xRh3B2

    Science.gov (United States)

    Vaidya, Ulhas; Dhar, S. K.; Thamizhavel, A.; Ramakrishnan, S.; Grover, A. K.

    2010-03-01

    The nonmagnetic rare earth ternary boride LaRh3B2, a hexagonal system of space group P6/mmm with CeCo3B2 type structure, is known to superconduct below Tc(0)˜ 2.7 K. When doped with Gd the Tc(0) reduces and no superconductivity is observed above 1.2 K with more than 15% (atomic) doping. The alloys, La0.8Gd0.2Rh3B2 and La0.6Gd0.4Rh3B2 show a jump at ˜ 70 K and rapid rise in magnetization below 10 K. However, no peaks are observed in at these temperatures in AC susceptibility which would have indicated magnetic ordering of the system. It is worth noting that GdRh3B2 orders ferromagnetically at ˜ 93 K. We shall present results of our investigations, above 2 K, in magnetic and transport properties of polycrystalline specimens LaxGd1-xRh3B2 (0 CeRh3B2 is a ferromagnet with Tc ˜ 115 K. The results of Gd doping in this reveal the survival of antiferromagnetic coupling between Ce and Gd spins.

  12. DNMT3b Modulates Melanoma Growth by Controlling Levels of mTORC2 Component RICTOR.

    Science.gov (United States)

    Micevic, Goran; Muthusamy, Viswanathan; Damsky, William; Theodosakis, Nicholas; Liu, Xiaoni; Meeth, Katrina; Wingrove, Emily; Santhanakrishnan, Manjula; Bosenberg, Marcus

    2016-03-08

    DNA methyltransferase DNMT3B is frequently overexpressed in tumor cells and plays important roles during the formation and progression of several cancer types. However, the specific signaling pathways controlled by DNMT3B in cancers, including melanoma, are poorly understood. Here, we report that DNMT3B plays a pro-tumorigenic role in human melanoma and that DNMT3B loss dramatically suppresses melanoma formation in the Braf/Pten mouse melanoma model. Loss of DNMT3B results in hypomethylation of the miR-196b promoter and increased miR-196b expression, which directly targets the mTORC2 component Rictor. Loss of RICTOR in turn prevents mTORC2 activation, which is critical for melanoma formation and growth. These findings establish Dnmt3b as a regulator of melanoma formation through its effect on mTORC2 signaling. Based on these results, DNMT3B is a potential therapeutic target in melanoma.

  13. Loss of Dnmt3b function upregulates the tumor modifier Ment and accelerates mouse lymphomagenesis.

    Science.gov (United States)

    Hlady, Ryan A; Novakova, Slavomira; Opavska, Jana; Klinkebiel, David; Peters, Staci L; Bies, Juraj; Hannah, Jay; Iqbal, Javeed; Anderson, Kristi M; Siebler, Hollie M; Smith, Lynette M; Greiner, Timothy C; Bastola, Dhundy; Joshi, Shantaram; Lockridge, Oksana; Simpson, Melanie A; Felsher, Dean W; Wagner, Kay-Uwe; Chan, Wing C; Christman, Judith K; Opavsky, Rene

    2012-01-01

    DNA methyltransferase 3B (Dnmt3b) belongs to a family of enzymes responsible for methylation of cytosine residues in mammals. DNA methylation contributes to the epigenetic control of gene transcription and is deregulated in virtually all human tumors. To better understand the generation of cancer-specific methylation patterns, we genetically inactivated Dnmt3b in a mouse model of MYC-induced lymphomagenesis. Ablation of Dnmt3b function using a conditional knockout in T cells accelerated lymphomagenesis by increasing cellular proliferation, which suggests that Dnmt3b functions as a tumor suppressor. Global methylation profiling revealed numerous gene promoters as potential targets of Dnmt3b activity, the majority of which were demethylated in Dnmt3b-/- lymphomas, but not in Dnmt3b-/- pretumor thymocytes, implicating Dnmt3b in maintenance of cytosine methylation in cancer. Functional analysis identified the gene Gm128 (which we termed herein methylated in normal thymocytes [Ment]) as a target of Dnmt3b activity. We found that Ment was gradually demethylated and overexpressed during tumor progression in Dnmt3b-/- lymphomas. Similarly, MENT was overexpressed in 67% of human lymphomas, and its transcription inversely correlated with methylation and levels of DNMT3B. Importantly, knockdown of Ment inhibited growth of mouse and human cells, whereas overexpression of Ment provided Dnmt3b+/+ cells with a proliferative advantage. Our findings identify Ment as an enhancer of lymphomagenesis that contributes to the tumor suppressor function of Dnmt3b and suggest it could be a potential target for anticancer therapies.

  14. Then and Now: Approaches to Understanding Children's Literature in Two Volumes

    Science.gov (United States)

    Bani-Khair, Baker M.; Khawaldeh, Imad M.

    2016-01-01

    This research paper investigates two main volumes taken from "Children's Literature Association Quarterly"; the earlier one is Vol. 11 published in 1986, and the other one, a more recent one, Vol. 32 published in 2007, as to understand the differences and similarities regarding the approaches used in the articles to understand Children's…

  15. Modulation of Dnmt3b function in vitro by interactions with Dnmt3L, Dnmt3a and Dnmt3b splice variants

    OpenAIRE

    Van Emburgh, Beth O.; Robertson, Keith D.

    2011-01-01

    DNA methylation, an essential regulator of transcription and chromatin structure, is established and maintained by the coordinated action of three DNA methyltransferases: DNMT1, DNMT3A and DNMT3B, and the inactive accessory factor DNMT3L. Disruptions in DNMT3B function are linked to carcinogenesis and genetic disease. DNMT3B is also highly alternatively spliced in a tissue- and disease-specific manner. The impact of intra-DNMT3 interactions and alternative splicing on the function of DNMT3 fa...

  16. Open Praxis vol. 8 issue 4

    Directory of Open Access Journals (Sweden)

    Inés Gil-Jaurena (ed., Various authors

    2016-12-01

    Full Text Available This last Open Praxis issue in 2016 is an open issue that includes four research papers, two innovative practice papers and two book reviews. It also includes the list of reviewers that have collaborated in volume 8.

  17. Keystone feasibility study. Final report. Vol. 4

    Energy Technology Data Exchange (ETDEWEB)

    1982-12-01

    Volume four of the Keystone coal-to-methanol project includes the following: (1) project management; (2) economic and financial analyses; (3) market analysis; (4) process licensing and agreements; and (5) appendices. 24 figures, 27 tables.

  18. AFRREV IJAH, Vol.3 (2) April, 2014

    African Journals Online (AJOL)

    Austin

    2014-04-10

    Apr 10, 2014 ... financial markets, particularly in high-inflation economies like. Nigeria. Nowadays .... for low trading volume and the small size of the ILBs market. Consequently, the .... the Middle East Technical University. HSBC Global ...

  19. Workshop Arboretum Volčji potok

    Directory of Open Access Journals (Sweden)

    Ana Kučan

    2012-01-01

    Full Text Available From its constitution onwards, the Volčji Potok Arboretum has been caught between various conflicting orientations. It is both a scientific, research and educational institution, and a cultural monument with exquisite garden and landscape design features and areas of great natural value and built cultural heritage, as well as commercial venue. At the same time, it functions as a park and an area for mass events, a garden centre and nursery. This variety of functions has helped Arboretum to survive the pressures of time; however, partial and uncoordinated interventions have threatened its original mission and its image and generated a number of conflicting situations. The workshop, organised on the initiative of the Institute for the Protection of Cultural Heritage of Slovenia, which involved students from the Faculty of Architecture and students from the Department of Landscape Architecture of the Biotechnical Faculty in mixed groups, generated eight proposals to solve some of the most urgent problems by introducing optimised development with clearly defined goals and priorities.

  20. Chile menos volátil

    Directory of Open Access Journals (Sweden)

    Felipe Larraín B.

    2008-01-01

    Full Text Available Desde fines de 1998 la economía chilena se desaceleró mucho. Si entre 1988 y 1998 la tasa de crecimiento promedio alcanzó un 7.8%, durante el período postcrisis asiática fue de sólo un 3.7%. Sin embargo, junto con la desaceleración del crecimiento, la volatilidad de la economía se redujo prácticamente a la mitad.Este trabajo investiga, mediante un análisis empírico formal, el papel que tuvieron la introducción de la flotación cambiaria y la utilización de la regla de superávit estructural en transformar a Chile en una economía menos volátil. Nuestros resultados muestran que la introducción de la regla fiscal parece haber reducido en un tercio la volatilidad del crecimiento del PIB. Por su parte, la flotación cambiaria contribuyó a reducir esa volatilidad adicionalmente en alrededor de un cuarto. Así, en conjunto, ambas medidas aparecen como responsables de haber disminuido la volatilidad del crecimiento de la economía chilena en casi 60%. Estos resultados son robustos ante especificaciones y posibles problemas de endogeneidad en algunas de las variables explicativas.

  1. Human Papillomavirus 16 E6 Upregulates APOBEC3B via the TEAD Transcription Factor.

    Science.gov (United States)

    Mori, Seiichiro; Takeuchi, Takamasa; Ishii, Yoshiyuki; Yugawa, Takashi; Kiyono, Tohru; Nishina, Hiroshi; Kukimoto, Iwao

    2017-03-15

    The cytidine deaminase APOBEC3B (A3B) underlies the genetic heterogeneity of several human cancers, including cervical cancer, which is caused by human papillomavirus (HPV) infection. We previously identified a region within the A3B promoter that is activated by the viral protein HPV16 E6 in human keratinocytes. Here, we discovered three sites recognized by the TEAD family of transcription factors within this region of the A3B promoter. Reporter assays in HEK293 cells showed that exogenously expressed TEAD4 induced A3B promoter activation through binding to these sites. Normal immortalized human keratinocytes expressing E6 (NIKS-E6) displayed increased levels of TEAD1/4 protein compared to parental NIKS. A series of E6 mutants revealed that E6-mediated degradation of p53 was important for increasing TEAD4 levels. Knockdown of TEADs in NIKS-E6 significantly reduced A3B mRNA levels, whereas ectopic expression of TEAD4 in NIKS increased A3B mRNA levels. Finally, chromatin immunoprecipitation assays demonstrated increased levels of TEAD4 binding to the A3B promoter in NIKS-E6 compared to NIKS. Collectively, these results indicate that E6 induces upregulation of A3B through increased levels of TEADs, highlighting the importance of the TEAD-A3B axis in carcinogenesis.IMPORTANCE The expression of APOBEC3B (A3B), a cellular DNA cytidine deaminase, is upregulated in various human cancers and leaves characteristic, signature mutations in cancer genomes, suggesting that it plays a prominent role in carcinogenesis. Viral oncoproteins encoded by human papillomavirus (HPV) and polyomavirus have been reported to induce A3B expression, implying the involvement of A3B upregulation in virus-associated carcinogenesis. However, the molecular mechanisms causing A3B upregulation remain unclear. Here, we demonstrate that exogenous expression of the cellular transcription factor TEAD activates the A3B promoter. Further, the HPV oncoprotein E6 increases the levels of endogenous TEAD1

  2. Degradation of the cancer genomic DNA deaminase APOBEC3B by SIV Vif.

    Science.gov (United States)

    Land, Allison M; Wang, Jiayi; Law, Emily K; Aberle, Ryan; Kirmaier, Andrea; Krupp, Annabel; Johnson, Welkin E; Harris, Reuben S

    2015-11-24

    APOBEC3B is a newly identified source of mutation in many cancers, including breast, head/neck, lung, bladder, cervical, and ovarian. APOBEC3B is a member of the APOBEC3 family of enzymes that deaminate DNA cytosine to produce the pro-mutagenic lesion, uracil. Several APOBEC3 family members function to restrict virus replication. For instance, APOBEC3D, APOBEC3F, APOBEC3G, and APOBEC3H combine to restrict HIV-1 in human lymphocytes. HIV-1 counteracts these APOBEC3s with the viral protein Vif, which targets the relevant APOBEC3s for proteasomal degradation. While APOBEC3B does not restrict HIV-1 and is not targeted by HIV-1 Vif in CD4-positive T cells, we asked whether related lentiviral Vif proteins could degrade APOBEC3B. Interestingly, several SIV Vif proteins are capable of promoting APOBEC3B degradation, with SIVmac239 Vif proving the most potent. This likely occurs through the canonical polyubiquitination mechanism as APOBEC3B protein levels are restored by MG132 treatment and by altering a conserved E3 ligase-binding motif. We further show that SIVmac239 Vif can prevent APOBEC3B mediated geno/cytotoxicity and degrade endogenous APOBEC3B in several cancer cell lines. Our data indicate that the APOBEC3B degradation potential of SIV Vif is an effective tool for neutralizing the cancer genomic DNA deaminase APOBEC3B. Further optimization of this natural APOBEC3 antagonist may benefit cancer therapy.

  3. DNA methyltransferase 3b is dispensable for mouse neural crest development.

    Directory of Open Access Journals (Sweden)

    Bridget T Jacques-Fricke

    Full Text Available The neural crest is a population of multipotent cells that migrates extensively throughout vertebrate embryos to form diverse structures. Mice mutant for the de novo DNA methyltransferase DNMT3b exhibit defects in two neural crest derivatives, the craniofacial skeleton and cardiac ventricular septum, suggesting that DNMT3b activity is necessary for neural crest development. Nevertheless, the requirement for DNMT3b specifically in neural crest cells, as opposed to interacting cell types, has not been determined. Using a conditional DNMT3b allele crossed to the neural crest cre drivers Wnt1-cre and Sox10-cre, neural crest DNMT3b mutants were generated. In both neural crest-specific and fully DNMT3b-mutant embryos, cranial neural crest cells exhibited only subtle migration defects, with increased numbers of dispersed cells trailing organized streams in the head. In spite of this, the resulting cranial ganglia, craniofacial skeleton, and heart developed normally when neural crest cells lacked DNMT3b. This indicates that DNTM3b is not necessary in cranial neural crest cells for their development. We conclude that defects in neural crest derivatives in DNMT3b mutant mice reflect a requirement for DNMT3b in lineages such as the branchial arch mesendoderm or the cardiac mesoderm that interact with neural crest cells during formation of these structures.

  4. Genetic ablation of NMDA receptor subunit NR3B in mouse reveals motoneuronal and nonmotoneuronal phenotypes.

    Science.gov (United States)

    Niemann, Stephan; Kanki, Hiroaki; Fukui, Yasuyuki; Takao, Keizo; Fukaya, Masahiro; Hynynen, Meri N; Churchill, Michael J; Shefner, Jeremy M; Bronson, Roderick T; Brown, Robert H; Watanabe, Masahiko; Miyakawa, Tsuyoshi; Itohara, Shigeyoshi; Hayashi, Yasunori

    2007-09-01

    NR3B is a modulatory subunit of the NMDA receptor, abundantly expressed in both cranial and spinal somatic motoneurons and at lower levels in other regions of the brain as well. Recently, we found the human NR3B gene (GRIN3B) to be highly genetically heterogeneous, and that approximately 10% of the normal European-American population lacks NR3B due to homozygous occurrence of a null allele in the gene. Therefore, it is especially important to understand the phenotypic consequences of the genetic loss of NR3B in both humans and animal models. We here provide results of behavioral analysis of mice genetically lacking NR3B, which is an ideal animal model due to homogeneity in genetic and environmental background. The NR3B(-/-) mice are viable and fertile. Consistent with the expression of NR3B in somatic motoneurons, the NR3B(-/-) mice showed a moderate but significant impairment in motor learning or coordination, and decreased activity in their home cages. Remarkably, the NR3B(-/-) mice showed a highly increased social interaction with their familiar cage mates in their home cage but moderately increased anxiety-like behaviour and decreased social interaction in a novel environment, consistent with the inhibitory role of NR3B on the functions of NMDA receptors. This work is the first reporting of the functional significance of NR3B in vivo and may give insight into the contribution of genetic variability of NR3B in the phenotypic heterogeneity among human population.

  5. 75 FR 910 - Airworthiness Directives; General Electric Company CF34-1A, -3A, -3A1, -3A2, -3B, and -3B1...

    Science.gov (United States)

    2010-01-07

    ... instructions related to operators who fly a regional jet (RJ) with the CF34-3A1 engine as a business jet (BJ.... We also estimate that it would take about 2 work-hours per engine to perform the required actions... Company CF34-1A, -3A, -3A1, -3A2, -3B, and -3B1 Turbofan Engines AGENCY: Federal Aviation...

  6. Overexpression of a splice variant of DNA methyltransferase 3b, DNMT3b4, associated with DNA hypomethylation on pericentromeric satellite regions during human hepatocarcinogenesis

    OpenAIRE

    Saito, Yoshimasa; Kanai, Yae; Sakamoto, Michiie; Saito, Hidetsugu; Ishii, Hiromasa; Hirohashi, Setsuo

    2002-01-01

    DNA hypomethylation on pericentromeric satellite regions is an early and frequent event associated with heterochromatin instability during human hepatocarcinogenesis. A DNA methyltransferase, DNMT3b, is required for methylation on pericentromeric satellite regions during mouse development. To clarify the molecular mechanism underlying DNA hypomethylation on pericentromeric satellite regions during human hepatocarcinogenesis, we examined mutations of the DNMT3b gene and mRNA expression levels ...

  7. African Journal for Physical Activity and Health Sciences - Vol 18 ...

    African Journals Online (AJOL)

    African Journal for Physical Activity and Health Sciences - Vol 18, No 4 ... Physical growth and academic intelligence of rural South African children: ... The influence of exergaming on the functional fitness in overweight and obese children ...

  8. Nigerian Journal of Family Practice - Vol 7, No 3 (2016)

    African Journals Online (AJOL)

    Nigerian Journal of Family Practice - Vol 7, No 3 (2016) ... underweight profile among adolescent secondary school students in Uyo, South-South, Nigeria ... Anthropometric characteristics and blood pressure levels of spouses of hypertensive ...

  9. Jolivet: Complete Flute Music, Vol. 2 / Guy S. Rickards

    Index Scriptorium Estoniae

    Rickards, Guy S.

    1996-01-01

    Uuest heliplaadist "Jolivet: Complete Flute Music, Vol. 2. Kroumata Percussion Ensemble, Tapiola Sinfonietta, Paavo Järvi". BIS CD 739 (64 minutes: DDD). Item marked from CD630 (6/94), CD272, remainder new to UK

  10. Research in Hospitality Management - Vol 6, No 1 (2016)

    African Journals Online (AJOL)

    Research in Hospitality Management - Vol 6, No 1 (2016) ... Employees, sustainability and motivation: Increasing employee engagement by addressing ... in the host-guest relationship · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT

  11. Journal of the Nigerian Association of Mathematical Physics - Vol 16 ...

    African Journals Online (AJOL)

    Journal of the Nigerian Association of Mathematical Physics - Vol 16 (2010) ... Construction of A Trial Function In The Variational Procedure of Quantum Mechanics ... of Breakthrough Time in Oil Recovery: Case of Some Niger Delta Reservoirs ...

  12. Nigerian Journal of Technology - Vol 35, No 2 (2016)

    African Journals Online (AJOL)

    Nigerian Journal of Technology - Vol 35, No 2 (2016) ... SENSITIVITY ANALYSIS ON FLEXIBLE ROAD PAVEMENT LIFE CYCLE COST MODEL · EMAIL .... WASTEWATER TREATMENT FACILITY OF A HOTEL IN PORT HARCOURT, RIVERS ...

  13. Journal of Development and Communication Studies - Vol 2, No 1 ...

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    Journal of Development and Communication Studies - Vol 2, No 1 (2012) ... Project of Kerala State, India · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT ... on the Chichewa Radio · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT

  14. Egyptian Journal of Biochemistry and Molecular Biology - Vol 29, No ...

    African Journals Online (AJOL)

    Egyptian Journal of Biochemistry and Molecular Biology - Vol 29, No 1 (2011) ... Resveratrol, a natural phytoalexin: A therapeutic promise for metabolic disorders in ... Biochemical study on some adipocyto-kines in chronic renal failure: Their ...

  15. Information Impact Vol. 6(3) Dec 2015

    African Journals Online (AJOL)

    2015-12-10

    Dec 10, 2015 ... Information Impact Vol. 6(3) Dec 2015 ... The study is on perceived factors influencing heads of libraries' choice of leadership styles in ... the leader rather than on leadership behavior ... effective administration in goal-seeking.

  16. Research in Hospitality Management - Vol 1, No 1 (2011)

    African Journals Online (AJOL)

    Research in Hospitality Management - Vol 1, No 1 (2011) ... Do social media display correct conventional hotel ratings? ... Employer branding: A new approach for the hospitality industry · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT

  17. International Journal of Engineering, Science and Technology - Vol ...

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    PROMOTING ACCESS TO AFRICAN RESEARCH ... International Journal of Engineering, Science and Technology - Vol 9, No 4 (2017) ... on MHD flow past a moving plate with Hall current · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT

  18. Journal of Language, Technology & Entrepreneurship in Africa Vol ...

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    enewa

    Journal of Language, Technology & Entrepreneurship in Africa Vol. 3. No. ... business skills in an effort to address a gap between theory and practice. ..... All in all more organizations appreciated the students efforts towards the work they had.

  19. African Journal of Environmental Science and Technology - Vol 4 ...

    African Journals Online (AJOL)

    African Journal of Environmental Science and Technology - Vol 4, No 11 (2010) ... Leaves of roadside plants as bioindicator of traffic related lead pollution during different ... Quality assessment of drinking water in Temeke District (part II): ...

  20. Journal of Applied Science, Engineering and Technology - Vol 4, No ...

    African Journals Online (AJOL)

    Journal of Applied Science, Engineering and Technology - Vol 4, No 2 (2004) ... Energy consumption pattern in palm kernel oil processing operations ... An improved solar cabinet dryer with natural convective heat transfer · EMAIL FULL TEXT ...

  1. Journal of Language, Technology & Entrepreneurship in Africa Vol ...

    African Journals Online (AJOL)

    ETSU

    Journal of Language, Technology & Entrepreneurship in Africa Vol. 3. No. 2 .... this, specific effects are created for hearers, which he called implicatures. .... Some speakers address each other by their first-born child's name, but whenever the.

  2. Nigerian Journal of Natural Products and Medicine - Vol 2 (1998)

    African Journals Online (AJOL)

    Nigerian Journal of Natural Products and Medicine - Vol 2 (1998) ... RESEARCH AND DEVELOPMENT INTO HERBAL MEDICINES · EMAIL FULL ... OF BRIDELIA FERRUGUBEA STEM BARK ON BLOOD CHEMISTRY AND HISTOLOGY OF ...

  3. Research in Hospitality Management - Vol 6, No 2 (2016)

    African Journals Online (AJOL)

    Research in Hospitality Management - Vol 6, No 2 (2016) ... Understanding young tourists' preferences for a wildlife tourism package ... Employees' perspectives of service quality in hotels · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT

  4. Journal of Research in Forestry, Wildlife and Environment - Vol 2 ...

    African Journals Online (AJOL)

    Journal of Research in Forestry, Wildlife and Environment - Vol 2, No 1 (2010) ... Diversity and Abundance of Fish Species in Gbedikere Lake, Bassa, Kogi State · EMAIL ... Fadama Users Group Characteristics that Influence Facilitators' Role ...

  5. African Journal of Biotechnology - Vol 14, No 6 (2015)

    African Journals Online (AJOL)

    African Journal of Biotechnology - Vol 14, No 6 (2015) ... molecular genetics in microbial degradation and decolorization of industrial waste water effluent ... Understanding the efficacy of influent waste water on microbial community structure of ...

  6. Jolivet: Complete Flute Music, Vol. 2 / Guy S. Rickards

    Index Scriptorium Estoniae

    Rickards, Guy S.

    1996-01-01

    Uuest heliplaadist "Jolivet: Complete Flute Music, Vol. 2. Kroumata Percussion Ensemble, Tapiola Sinfonietta, Paavo Järvi". BIS CD 739 (64 minutes: DDD). Item marked from CD630 (6/94), CD272, remainder new to UK

  7. AFRREV IJAH: An International Journal of Arts and Humanities - Vol ...

    African Journals Online (AJOL)

    PROMOTING ACCESS TO AFRICAN RESEARCH ... AFRREV IJAH: An International Journal of Arts and Humanities - Vol 6, No 2 (2017) ... Taylor and Francis Journals under the critical lens of readability analysis ... Current Issue Atom logo

  8. International Journal of Emotional Psychology and Sport Ethics - Vol ...

    African Journals Online (AJOL)

    PROMOTING ACCESS TO AFRICAN RESEARCH ... International Journal of Emotional Psychology and Sport Ethics - Vol 10 (2008) ... Re-Inventing The Current 'Ss' English Language Curriculum: Implications For Students' Performance In ...

  9. African Research Review - Vol 11, No 3 (2017)

    African Journals Online (AJOL)

    African Research Review - Vol 11, No 3 (2017) ... Targeted or Restrictive: Impact of U.S. and EU Sanctions on Education and ... Revamping Nigerian Economy through Cassava Production · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT

  10. Mizan Law Review - Vol 11, No 1 (2017)

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    Mizan Law Review - Vol 11, No 1 (2017) ... Interrogating the economy-first paradigm in 'Sustainable Development': towards ... lessons from the Uniform Commercial Code of the US · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT

  11. ∆ DNMT3B4-del Contributes to Aberrant DNA Methylation Patterns in Lung Tumorigenesis

    OpenAIRE

    Ma, Mark Z.; Ruxian Lin; José Carrillo; Manisha Bhutani; Ashutosh Pathak; Hening Ren; Yaokun Li; Jiuzhou Song; Li Mao

    2015-01-01

    Aberrant DNA methylation is a hallmark of cancer but mechanisms contributing to the abnormality remain elusive. We have previously shown that ∆DNMT3B is the predominantly expressed form of DNMT3B. In this study, we found that most of the lung cancer cell lines tested predominantly expressed DNMT3B isoforms without exons 21, 22 or both 21 and 22 (a region corresponding to the enzymatic domain of DNMT3B) termed DNMT3B/∆DNMT3B-del. In normal bronchial epithelial cells, DNMT3B/ΔDNMT3B and DNMT3B/...

  12. Inefficient binding of IgM immune complexes to erythrocyte C3b-C4b receptors (CR1) and weak incorporation of C3b-iC3b into the complexes

    DEFF Research Database (Denmark)

    Kávai, M; Rasmussen, J M; Baatrup, G

    1988-01-01

    The binding of soluble complement-reacted IgM immune complexes (IC) to erythrocyte (E) C3b-C4b receptors (CR1) and the incorporation of C3b-iC3b into solid phase IgM-IC was investigated. The optimal binding of liquid phase IgM-IC to E-CR1 was obtained with IC formed at moderate antibody excess......, but the binding was low (2-3%) when compared to the binding of the corresponding IgG-IC (50-60%). Solid phase IC were prepared by coating microwells with heat-aggregated bovine serum albumin (BSA) followed by incubation with rabbit IgM anti-BSA antibody. The IC were reacted with human serum at 37 degrees C...

  13. Transcriptomic Characterization of SF3B1 Mutation Reveals Its Pleiotropic Effects in Chronic Lymphocytic Leukemia.

    Science.gov (United States)

    Wang, Lili; Brooks, Angela N; Fan, Jean; Wan, Youzhong; Gambe, Rutendo; Li, Shuqiang; Hergert, Sarah; Yin, Shanye; Freeman, Samuel S; Levin, Joshua Z; Fan, Lin; Seiler, Michael; Buonamici, Silvia; Smith, Peter G; Chau, Kevin F; Cibulskis, Carrie L; Zhang, Wandi; Rassenti, Laura Z; Ghia, Emanuela M; Kipps, Thomas J; Fernandes, Stacey; Bloch, Donald B; Kotliar, Dylan; Landau, Dan A; Shukla, Sachet A; Aster, Jon C; Reed, Robin; DeLuca, David S; Brown, Jennifer R; Neuberg, Donna; Getz, Gad; Livak, Kenneth J; Meyerson, Matthew M; Kharchenko, Peter V; Wu, Catherine J

    2016-11-14

    Mutations in SF3B1, which encodes a spliceosome component, are associated with poor outcome in chronic lymphocytic leukemia (CLL), but how these contribute to CLL progression remains poorly understood. We undertook a transcriptomic characterization of primary human CLL cells to identify transcripts and pathways affected by SF3B1 mutation. Splicing alterations, identified in the analysis of bulk cells, were confirmed in single SF3B1-mutated CLL cells and also found in cell lines ectopically expressing mutant SF3B1. SF3B1 mutation was found to dysregulate multiple cellular functions including DNA damage response, telomere maintenance, and Notch signaling (mediated through KLF8 upregulation, increased TERC and TERT expression, or altered splicing of DVL2 transcript, respectively). SF3B1 mutation leads to diverse changes in CLL-related pathways.

  14. Identification of Semaphorin3B as a Direct Target of p53

    Directory of Open Access Journals (Sweden)

    Kensuke Ochi

    2002-01-01

    Full Text Available A cDNA microarray analysis indicated that Semaphorin3B. (20Sema3B, a gene whose product is involved in axon guidance and axonal repulsion, is inducible by p53. Introduction of exogenous p53 into a glioblastoma cell line lacking wild-type p53. (20U373MG dramatically induced expression of Sema3B mRNA. An electrophoretic mobility shift assay and a reporter assay confirmed that a potential p53 binding site present in the promoter region had p53-dependent transcriptional activity. Expression of endogenous Sema3B was induced in response to genotoxic stresses caused by adriamycin treatment or UV irradiation in a p53-dependent manner. Ectopic expression of Sema3B in p53-defective cells reduced the number of colonies in colony formation assays. These results suggest that Sema3B might play some role in regulating cell growth as a mediator of p53 tumor- suppressor activity.

  15. Isothermal reduction kinetics of Panzhihua ilmenite concentrate under 30vol% CO-70vol% N2 atmosphere

    Science.gov (United States)

    Zhang, Ying-yi; Lü, Wei; Lü, Xue-wei; Li, Sheng-ping; Bai, Chen-guang; Song, Bing; Han, Ke-xi

    2017-03-01

    The reduction of ilmenite concentrate in 30vol% CO-70vol% N2 atmosphere was characterized by thermogravimetric and differential thermogravimetric (TG-DTG) analysis methods at temperatures from 1073 to 1223 K. The isothermal reduction results show that the reduction process comprised two stages; the corresponding apparent activation energy was obtained by the iso-conversional and model-fitting methods. For the first stage, the effect of temperature on the conversion degree was not obvious, the phase boundary chemical reaction was the controlling step, with an apparent activation energy of 15.55-40.71 kJ·mol-1. For the second stage, when the temperatures was greater than 1123 K, the reaction rate and the conversion degree increased sharply with increasing temperature, and random nucleation and subsequent growth were the controlling steps, with an apparent activation energy ranging from 182.33 to 195.95 kJ·mol-1. For the whole reduction process, the average activation energy and pre-exponential factor were 98.94-118.33 kJ·mol-1 and 1.820-1.816 min-1, respectively.

  16. Dehybridization of the 4f shell in Si-substituted CeRh3B2

    Science.gov (United States)

    Malik, S. K.; Shenoy, G. K.; Dhar, S. K.; Paulose, P. L.; Vijayaraghavan, R.

    1986-12-01

    The compound CeRh3B2 is known to order magnetically with an unusually high Curie temperature (TC) of 115 K and a low saturation moment of about 0.4μB. We have studied the magnetic behavior of the system CeRh3(B1-xSix)2 (0CeRh3B2 arises because of the Ce 4f hybridization with the neighboring ligands.

  17. Anomalous magnetism of CeRh3B2 under pressure

    Science.gov (United States)

    Shaheen, S. A.; Schilling, J. S.; Klavins, P.; Vining, C. B.; Shelton, R. N.

    Whereas CeRh3B2 possesses the highest magnetic ordering temperature (T sub c approx. = 115K) of any Ce-compound with nonmagnetic constituents, LaRh3B2 becomes superconducting below 2.5K. Recent magnetization measurements under pressure as well as lattice parameter and specific heat studies shed light on the nature of the anomalous magnetic state in CeRh3B2.

  18. Role of DNMT3B in the regulation of early neural and neural crest specifiers.

    Science.gov (United States)

    Martins-Taylor, Kristen; Schroeder, Diane I; LaSalle, Janine M; Lalande, Marc; Xu, Ren-He

    2012-01-01

    The de novo DNA methyltransferase DNMT3B functions in establishing DNA methylation patterns during development. DNMT3B missense mutations cause immunodeficiency, centromere instability and facial anomalies (ICF) syndrome. The restriction of Dnmt3b expression to neural progenitor cells, as well as the mild cognitive defects observed in ICF patients, suggests that DNMT3B may play an important role in early neurogenesis. We performed RNAi knockdown of DNMT3B in human embryonic stem cells (hESCs) in order to investigate the mechanistic contribution of DNMT3B to DNA methylation and early neuronal differentiation. While DNMT3B was not required for early neuroepithelium specification, DNMT3B deficient neuroepithelium exhibited accelerated maturation with earlier expression, relative to normal hESCs, of mature neuronal markers (such as NEUROD1) and of early neuronal regional specifiers (such as those for the neural crest). Genome-wide analyses of DNA methylation by MethylC-seq identified novel regions of hypomethylation in the DNMT3B knockdowns along the X chromosome as well as pericentromeric regions, rather than changes to promoters of specific dysregulated genes. We observed a loss of H3K27me3 and the polycomb complex protein EZH2 at the promoters of early neural and neural crest specifier genes during differentiation of DNMT3B knockdown but not normal hESCs. Our results indicate that DNMT3B mediates large-scale methylation patterns in hESCs and that DNMT3B deficiency in the cells alters the timing of their neuronal differentiation and maturation.

  19. miR-148 targets human DNMT3b protein coding region.

    Science.gov (United States)

    Duursma, Anja M; Kedde, Martijn; Schrier, Mariette; le Sage, Carlos; Agami, Reuven

    2008-05-01

    MicroRNAs (miRNAs) are small noncoding RNA molecules of 20-24 nucleotides that regulate gene expression. In animals, miRNAs form imperfect interactions with sequences in the 3' Untranslated region (3'UTR) of mRNAs, causing translational inhibition and mRNA decay. In contrast, plant miRNAs mostly associate with protein coding regions. Here we show that human miR-148 represses DNA methyltransferase 3b (Dnmt3b) gene expression through a region in its coding sequence. This region is evolutionary conserved and present in the Dnmt3b splice variants Dnmt3b1, Dnmt3b2, and Dnmt3b4, but not in the abundantly expressed Dnmt3b3. Whereas overexpression of miR-148 results in decreased DNMT3b1 expression, short-hairpin RNA-mediated miR-148 repression leads to an increase in DNMT3b1 expression. Interestingly, mutating the putative miR-148 target site in Dnmt3b1 abolishes regulation by miR-148. Moreover, endogenous Dnmt3b3 mRNA, which lacks the putative miR-148 target site, is resistant to miR-148-mediated regulation. Thus, our results demonstrate that the coding sequence of Dnmt3b mediates regulation by the miR-148 family. More generally, we provide evidence that coding regions of human genes can be targeted by miRNAs, and that such a mechanism might play a role in determining the relative abundance of different splice variants.

  20. Structural determinants of APOBEC3B non-catalytic domain for molecular assembly and catalytic regulation

    Energy Technology Data Exchange (ETDEWEB)

    Xiao, Xiao; Yang, Hanjing; Arutiunian, Vagan; Fang, Yao; Besse, Guillaume; Morimoto, Cherie; Zirkle, Brett; Chen, Xiaojiang S. (USC)

    2017-05-30

    The catalytic activity of human cytidine deaminase APOBEC3B (A3B) has been correlated with kataegic mutational patterns within multiple cancer types. The molecular basis of how the N-terminal non-catalytic CD1 regulates the catalytic activity and consequently, biological function of A3B remains relatively unknown. Here, we report the crystal structure of a soluble human A3B-CD1 variant and delineate several structural elements of CD1 involved in molecular assembly, nucleic acid interactions and catalytic regulation of A3B. We show that (i) A3B expressed in human cells exists in hypoactive high-molecular-weight (HMW) complexes, which can be activated without apparent dissociation into low-molecular-weight (LMW) species after RNase A treatment. (ii) Multiple surface hydrophobic residues of CD1 mediate the HMW complex assembly and affect the catalytic activity, including one tryptophan residue W127 that likely acts through regulating nucleic acid binding. (iii) One of the highly positively charged surfaces on CD1 is involved in RNA-dependent attenuation of A3B catalysis. (iv) Surface hydrophobic residues of CD1 are involved in heterogeneous nuclear ribonucleoproteins (hnRNPs) binding to A3B. The structural and biochemical insights described here suggest that unique structural features on CD1 regulate the molecular assembly and catalytic activity of A3B through distinct mechanisms.

  1. DNA methyltransferase 3B expression is associated with poor outcome of stage I testicular seminoma

    Science.gov (United States)

    Arai, Eri; Nakagawa, Tohru; Wakai-Ushijima, Saori; Fujimoto, Hiroyuki; Kanai, Yae

    2012-01-01

    Aims To examine in testicular seminomas the expression of DNA methyltransferase 3B (DNMT3B), which is known to be associated with early embryonic development and carcinogenesis, and to obtain a predictive marker for relapse of stage I seminomas. Methods and results Immunohistochemical examination of DNMT3B was performed in 88 cases of seminoma, 35 (39.8%) of which showed widely scattered nuclear immunoreactivity for DNMT3B, and 53 (60.2%) of which were completely negative. The incidence of focal DNMT3B expression was higher in stage III seminomas (5/5, 100%) than in stage I (25/70, 35.7%) or stage II (5/13, 38.5%) seminomas (P = 0.011). In stage I seminomas there were no significant correlations between DNMT3B expression and tumour size, invasion of the rete testis, or lymphatic or vascular involvement. Six of 25 cases (24%) showing DNMT3B expression relapsed, whereas only 3/45 cases (6.7%) lacking such expression did so (P = 0.037). Patients with seminomas showing DNMT3B expression had a significantly lower relapse-free survival rate than patients whose tumours lacked this feature (P = 0.0464). Conclusions Patients with seminomas showing focal DNMT3B expression are at increased risk of relapse, and should be followed up carefully. PMID:22394436

  2. Identification and characterization of cul-3b, a novel hominine CUL-3 transcript variant

    Institute of Scientific and Technical Information of China (English)

    Li Lu; Zuo-Ming Zhou; Xiao-Yan Huang; Min Xu; Lan-Lan Yin; Hui Wang; Zhi-Yang Xu; Jia-Hao Sha

    2005-01-01

    Aim: To identify genes related to the human testis development by substrate hybridization technique. Methods: A human testis cDNA microarray was constructed and hybridized with probes prepared from human adult and fetal testes and spermatozoa mRNAs by reverse transcription reactions. The differentially expressed genes were sequenced.And a newly identified cullin-3 (CUL-3) transcript variant (designated cul-3b) was bio-informatically analyzed with an online GenBank database. Multi-tissue reverse transcription polymerase chain reaction (RT-PCR) was used to determine the tissue expression profile of cul-3b. Results: Cul-3b, a novel CUL-3 transcript variant, was identified. The expression level of cul-3b in adult testes was 3.79-fold higher than that in fetal ones. Cul-3b differed from cul-3(including NM_003590 and AY337761) in the opening reading frame and had three internal ribosomal entry sites (IRESes) in the 5'-UTR. These led to a 24 amino acid (aa) truncation at N-terminus of CUL-3b as compared with CUL-3 and a more motivated expression pattern of cul-3b under some strict circumstances. Additionally, cul-3b expressed ubiquitously in human tissues according to multi-tissue RT-PCR. Conclusion: Cul-3b is a novel transcript variant of CUL-3, which may be important not only for the development of human testis but also for that of other organs.

  3. The 'de novo' DNA methyltransferase Dnmt3b compensates the Dnmt1-deficient intestinal epithelium.

    Science.gov (United States)

    Elliott, Ellen N; Sheaffer, Karyn L; Kaestner, Klaus H

    2016-01-25

    Dnmt1 is critical for immediate postnatal intestinal development, but is not required for the survival of the adult intestinal epithelium, the only rapidly dividing somatic tissue for which this has been shown. Acute Dnmt1 deletion elicits dramatic hypomethylation and genomic instability. Recovery of DNA methylation state and intestinal health is dependent on the de novo methyltransferase Dnmt3b. Ablation of both Dnmt1 and Dnmt3b in the intestinal epithelium is lethal, while deletion of either Dnmt1 or Dnmt3b has no effect on survival. These results demonstrate that Dnmt1 and Dnmt3b cooperate to maintain DNA methylation and genomic integrity in the intestinal epithelium.

  4. Antiproliferative effects of DNA methyltransferase 3B depletion are not associated with DNA demethylation.

    Directory of Open Access Journals (Sweden)

    Sabine Hagemann

    Full Text Available Silencing of genes by hypermethylation contributes to cancer progression and has been shown to occur with increased frequency at specific genomic loci. However, the precise mechanisms underlying the establishment and maintenance of aberrant methylation marks are still elusive. The de novo DNA methyltransferase 3B (DNMT3B has been suggested to play an important role in the generation of cancer-specific methylation patterns. Previous studies have shown that a reduction of DNMT3B protein levels induces antiproliferative effects in cancer cells that were attributed to the demethylation and reactivation of tumor suppressor genes. However, methylation changes have not been analyzed in detail yet. Using RNA interference we reduced DNMT3B protein levels in colon cancer cell lines. Our results confirm that depletion of DNMT3B specifically reduced the proliferation rate of DNMT3B-overexpressing colon cancer cell lines. However, genome-scale DNA methylation profiling failed to reveal methylation changes at putative DNMT3B target genes, even in the complete absence of DNMT3B. These results show that DNMT3B is dispensable for the maintenance of aberrant DNA methylation patterns in human colon cancer cells and they have important implications for the development of targeted DNA methyltransferase inhibitors as epigenetic cancer drugs.

  5. De novo DNA methyltransferase DNMT3b interacts with NEDD8-modified proteins.

    Science.gov (United States)

    Shamay, Meir; Greenway, Melanie; Liao, Gangling; Ambinder, Richard F; Hayward, S Diane

    2010-11-19

    DNA methylation and histone modifications play an important role in transcription regulation. In cancer cells, many promoters become aberrantly methylated through the activity of the de novo DNA methyltransferases DNMT3a and DNMT3b and acquire repressive chromatin marks. NEDD8 is a ubiquitin-like protein modifier that is conjugated to target proteins, such as cullins, to regulate their activity, and cullin 4A (CUL4A) in its NEDD8-modified form is essential for repressive chromatin formation. We found that DNMT3b associates with NEDD8-modified proteins. Whereas DNMT3b interacts directly in vitro with NEDD8, conjugation of NEDD8 to target proteins enhances this interaction in vivo. DNMT3b immunoprecipitated two major bands of endogenously NEDDylated proteins at the size of NEDDylated cullins, and indeed DNMT3b interacted with CUL1, CUL2, CUL3, CUL4A, and CUL5. Moreover, DNMT3b preferentially immunoprecipitated the NEDDylated form of endogenous CUL4A. NEDD8 enhanced DNMT3b-dependent DNA methylation. Chromatin immunoprecipitation assays suggest that DNMT3b recruits CUL4A and NEDD8 to chromatin, whereas deletion of Dnmt3b reduces the association of CUL4A and NEDD8 at a repressed promoter in a cancer cell line.

  6. Dnmt3b is a haploinsufficient tumor suppressor gene in Myc-induced lymphomagenesis.

    Science.gov (United States)

    Vasanthakumar, Aparna; Lepore, Janet B; Zegarek, Matthew H; Kocherginsky, Masha; Singh, Mahi; Davis, Elizabeth M; Link, Petra A; Anastasi, John; Le Beau, Michelle M; Karpf, Adam R; Godley, Lucy A

    2013-03-14

    The drivers of abnormal DNA methylation in human cancers include widespread aberrant splicing of the DNMT3B gene, producing abnormal transcripts that encode truncated proteins that may act as dominant negative isoforms. To test whether reduced Dnmt3b dosage can alter tumorigenesis, we bred Dnmt3b(+/-) mice to Eµ-Myc mice, a mouse model susceptible to B-cell lymphomas. Eµ-Myc/Dnmt3b(+/-) mice showed a dramatic acceleration of lymphomagenesis, greater even than that observed in Eµ-Myc mice that express a truncated DNMT3B isoform found in human tumors, DNMT3B7. This finding indicates that Dnmt3b can act as a haploinsufficient tumor suppressor gene. Although reduction in both Dnmt3b dosage and expression of DNMT3B7 within the Eµ-Myc system had similar effects on tumorigenesis and DNA hypermethylation, different molecular mechanisms appear to underlie these changes. This study offers insight into how de novo DNA methyltransferases function as tumor suppressors and the sensitivity of Myc-induced lymphomas to DNA methylation.

  7. Annual Review of Psychology, Vol. 29, 1978.

    Science.gov (United States)

    Rosenzweig, Mark R., Ed.; Porter, Lyman W., Ed.

    The volume contains 20 scholarly essays on current research in representative areas of the field of psychology. Most of the authors are professors and researchers at universities in the United States, representing departments of psychology, management, social ecology, human development, education, psychiatry, and medicine. A few private research…

  8. Minicourses in Astrophysics, Modular Approach, Vol. II.

    Science.gov (United States)

    Illinois Univ., Chicago.

    This is the second of a two-volume minicourse in astrophysics. It contains chapters on the following topics: stellar nuclear energy sources and nucleosynthesis; stellar evolution; stellar structure and its determination; and pulsars. Each chapter gives much technical discussion, mathematical treatment, diagrams, and examples. References are…

  9. Culture of Schools. Final Report. Volume II

    Science.gov (United States)

    American Anthropological Association, Washington, DC.

    Volume II of this 4-volume report contains the second half of a report on the Conference on the Culture of Schools held at Greystone, New York, (the first half of the conference report appears in Vol. I, SP 003 900), and the first part of a report on the Colloquium on the Culture of Schools held at the New School for Social Research in 1966. (The…

  10. SF3B1 haploinsufficiency leads to formation of ring sideroblasts in myelodysplastic syndromes.

    Science.gov (United States)

    Visconte, Valeria; Rogers, Heesun J; Singh, Jarnail; Barnard, John; Bupathi, Manoj; Traina, Fabiola; McMahon, James; Makishima, Hideki; Szpurka, Hadrian; Jankowska, Anna; Jerez, Andres; Sekeres, Mikkael A; Saunthararajah, Yogen; Advani, Anjali S; Copelan, Edward; Koseki, Haruhiko; Isono, Kyoichi; Padgett, Richard A; Osman, Sami; Koide, Kazunori; O'Keefe, Christine; Maciejewski, Jaroslaw P; Tiu, Ramon V

    2012-10-18

    Whole exome/genome sequencing has been fundamental in the identification of somatic mutations in the spliceosome machinery in myelodysplastic syndromes (MDSs) and other hematologic disorders. SF3B1, splicing factor 3b subunit 1 is mutated in 60%-80% of refractory anemia with ring sideroblasts (RARS) and RARS associated with thrombocytosis (RARS-T), 2 distinct subtypes of MDS and MDS/myeloproliferative neoplasms (MDSs/MPNs). An idiosyncratic feature of RARS/RARS-T is the presence of abnormal sideroblasts characterized by iron overload in the mitochondria, called RS. Based on the high frequency of mutations of SF3B1 in RARS/RARS-T, we investigated the consequences of SF3B1 alterations. Ultrastructurally, SF3B1 mutants showed altered iron distribution characterized by coarse iron deposits compared with wild-type RARS patients by transmission electron microscopy. SF3B1 knockdown experiments in K562 cells resulted in down-regulation of U2-type intron-splicing by RT-PCR. RNA-sequencing analysis of SF3B1 mutants showed differentially used genes relevant in MDS pathogenesis, such as ASXL1, CBL, EZH, and RUNX families. A SF3B pharmacologic inhibitor, meayamycin, induced the formation of RS in healthy BM cells. Further, BM aspirates of Sf3b1 heterozygous knockout mice showed RS by Prussian blue. In conclusion, we report the first experimental evidence of the association between SF3B1 and RS phenotype. Our data suggest that SF3B1 haploinsufficiency leads to RS formation.

  11. Mutation Processes in 293-Based Clones Overexpressing the DNA Cytosine Deaminase APOBEC3B.

    Directory of Open Access Journals (Sweden)

    Monica K Akre

    Full Text Available Molecular, cellular, and clinical studies have combined to demonstrate a contribution from the DNA cytosine deaminase APOBEC3B (A3B to the overall mutation load in breast, head/neck, lung, bladder, cervical, ovarian, and other cancer types. However, the complete landscape of mutations attributable to this enzyme has yet to be determined in a controlled human cell system. We report a conditional and isogenic system for A3B induction, genomic DNA deamination, and mutagenesis. Human 293-derived cells were engineered to express doxycycline-inducible A3B-eGFP or eGFP constructs. Cells were subjected to 10 rounds of A3B-eGFP exposure that each caused 80-90% cell death. Control pools were subjected to parallel rounds of non-toxic eGFP exposure, and dilutions were done each round to mimic A3B-eGFP induced population fluctuations. Targeted sequencing of portions of TP53 and MYC demonstrated greater mutation accumulation in the A3B-eGFP exposed pools. Clones were generated and microarray analyses were used to identify those with the greatest number of SNP alterations for whole genome sequencing. A3B-eGFP exposed clones showed global increases in C-to-T transition mutations, enrichments for cytosine mutations within A3B-preferred trinucleotide motifs, and more copy number aberrations. Surprisingly, both control and A3B-eGFP clones also elicited strong mutator phenotypes characteristic of defective mismatch repair. Despite this additional mutational process, the 293-based system characterized here still yielded a genome-wide view of A3B-catalyzed mutagenesis in human cells and a system for additional studies on the compounded effects of simultaneous mutation mechanisms in cancer cells.

  12. Analysis of DNA methylation change induced by Dnmt3b in mouse hepatocytes.

    Science.gov (United States)

    Takahashi, Mayumi; Kamei, Yasutomi; Ehara, Tatsuya; Yuan, Xunmei; Suganami, Takayoshi; Takai-Igarashi, Takako; Hatada, Izuho; Ogawa, Yoshihiro

    2013-05-17

    DNA methylation is a key epigenetic contributor to gene regulation in mammals. We have recently found that in the mouse liver, the promoter region of glycerol-3-phosphate acyltransferase 1, a rate-limiting enzyme of de novo lipogenesis, is regulated by DNA methylation, which is mediated by Dnmt3b, an enzyme required for the initiation of de novo methylation. In this study, using primary cultures of mouse hepatocytes with adenoviral overexpression of Dnmt3b, we characterized Dnmt3b-dependent DNA methylation on a genome-wide basis. A genome-wide DNA methylation analysis, called microarray-based integrated analysis of methylation by isoschizomers, identified 108 genes with Dnmt3b dependent DNA methylation. In DNA expression array analysis, expression of some genes with Dnmt3b-dependent DNA methylation was suppressed. Studies with primary mouse hepatocytes overexpressing Dnmt3b or Dnmt3a revealed that many genes with Dnmt3b-dependent methylation are not methylated by Dnmt3a, whereas those methylated by Dnmt3a are mostly methylated by Dnmt3b. Bioinformatic analysis showed that the CANAGCTG and CCGGWNCSC (N denotes A, T, G, or C; W denotes A or T; and S denotes C or G) sequences are enriched in genes methylated by overexpression of Dnmt3b and Dnmt3a, respectively. We also observed a large number of genes with Dnmt3b-dependent DNA methylation in primary cultures of mouse hepatocytes with adenoviral overexpression of Dnmt3, suggesting that Dnmt3b is an important DNA methyltransferase in primary mouse hepatocytes, targets specific genes, and potentially plays a role in vivo.

  13. Reposição volêmica em pacientes politraumatizados

    Directory of Open Access Journals (Sweden)

    Delmo Sakabe

    2007-05-01

    Full Text Available O debate sobre a administração de colóides, cristalóides, soluções hipertônicas ou elementos sanguíneos em pacientes críticos estende-se desde o século 19. Algumas dessas soluções podem ser diferentemente distribuídas nos espaços intra e extravasculares, assim como nos espaços intra e extracelulares, causando diferentes efeitos fisiológicos. Diversos estudos foram realizados com o objetivo de comparar um fluido ao outro, mostrando a eficácia e as vantagens de cada um. Porém, não existe um trabalho que consiga provar uma diferença significativa em relação à morbi-mortalidade após a reposição volêmica com cada tipo de fluido. Esses estudo, por serem diferentes em relação aos protocolos de ressucitação, volumes e tipos de fluidos utilizados, dificultam a comparação dos resultados; no entanto, sabe-se que a reposição volêmica depende de cada situação clínica. A utilização da reposição volêmica pré-hospitalar é discutível, visto que pode aumentar o sangramento ou mesmo ser motivo de atraso na transferência do paciente ao hospital, ao mesmo tempo em que pode reduzir o risco de isquemia tecidual. Uma vez que a hemorragia tenha sido controlada, há um consenso para o fato de que o volume intravascular deve ser reposto da forma mais rápida e eficaz possível para minimizar a quantidade de células afetadas diretamente pela má perfusão tecidual. Os atuais trabalhos envolvidos nessa polêmica trazem resultados que sugerem a continuidade das investigações, visto que nenhum deles atingiu um ponto final comum. Desses estudos, um aspecto com o qual todos concordam e que se tornou claro é que não existe uma fórmula mágica única de administração intravenosa para os pacientes politraumatizados. Este trabalho visa, por meio de revisão bibliográfica, mostrar os tipos de fluidos a serem utilizados para reposição volêmica de pacientes politraumatizados, assim como os meios e a forma de utilização dos

  14. Nuclear- and radiochemistry. Vol. 2. Modern applications

    Energy Technology Data Exchange (ETDEWEB)

    Roesch, Frank (ed.) [Mainz Univ. (Germany). Inst. fuer Kernchemie

    2016-07-01

    This work is conceived to meet the demand of state-of-the-art literature to teach the fundamentals as well as the modern applications of nuclear chemistry. The work will consist of two volumes: the first one covering the basics of nuclear chemistry such as the relevant parameters of instable atomic nuclei, the various modi of radioactive transmutations, the corresponding types of radiation including their detection and dosimetry, and finally the mechanisms of nuclear reactions. The second volume addresses relevant fields of nuclear chemistry, such as the chemistry of radioactive elements, application of radioactive nuclei in life sciences, nuclear energy, waste managements and environmental aspects, radiochemical separations, radioanalytical and spectroscopic methods, etc. Here, leading experts will contribute up-to-date knowledge on the most important application of nuclear chemistry.

  15. LM-3B/E Launched Eutelsat's W3C Satellite

    Institute of Scientific and Technical Information of China (English)

    Bian Ji

    2011-01-01

    At 16:21 Beijing time on October 7,2011,China successfully launched the W3C commercial telecommunications satellite manufactured by Thales Alenia Space for Eutelsat Communications into geostationary transfer orbit by using an enhanced LM-3B (LM-3B/E) launch vehicle from Xichang Satellite Launch Center.

  16. A novel role of KIF3b in the seminoma cell cycle.

    Science.gov (United States)

    Shen, Hao-Qing; Xiao, Yu-Xi; She, Zhen-Yu; Tan, Fu-Qing; Yang, Wan-Xi

    2017-03-01

    KIF3b is a protein of the kinesin-2 family which plays an important role in intraflagellar transport. Testis cancer is a common cancer among young men. Its diagnostic rate is increasing and over half of the cases are seminomas. Many aspects of the mechanism and gene expression background of this cancer remain unclear. Using western-blotting and semi-quantitative PCR we found high protein levels of KIF3b enrichment in seminoma tissue despite the mRNA levels remaining equivalent to that of normal testicular tissues. The distribution of KIF3b was mainly in cells with division potential. Wound-healing assays and cell counting kit assays showed that the knockdown of KIF3b significantly suppressed cell migration ability, viability and number in HeLa cells. Immunofluorescence images during the cell cycle revealed that KIF3b tended to gather at the spindles and was enriched at the central spindle. This indicated that KIF3b may also have direct impacts upon spindle formation and cytokinesis. By counting the numbers of nuclei, spindles and cells, we found that the rates of multipolar division and multi-nucleation were raised in KIF3b-knockdown cells. In this way we demonstrate that KIF3b functions importantly in mitosis and may be essential to seminoma cell division and proliferation as well as being necessary for normal cell division. Copyright © 2017 Elsevier Inc. All rights reserved.

  17. Evidence that deletion at FCGR3B is a risk factor for systemic sclerosis

    NARCIS (Netherlands)

    McKinney, C.; Broen, J.C.A.; Vonk, M.C.; Beretta, L.; Hesselstrand, R.; Hunzelmann, N.; Riemekasten, G.; Scorza, R.; Simeon, C.P.; Fonollosa, V.; Carreira, P.E.; Ortego-Centeno, N.; Gonzalez-Gay, M.A.; Airo, P.; Coenen, M.J.; Martin, J.; Radstake, T.R.D.J.; Merriman, T.R.

    2012-01-01

    There is increasing evidence that gene copy number (CN) variation influences clinical phenotype. The low-affinity Fc receptor 3B (FCGR3B) located in the FCGR gene cluster is a CN polymorphic gene involved in the recruitment of polymorphonuclear neutrophils to sites of inflammation and their activati

  18. Magnetism in the system Ce1-xLaxRh3B2 (abstract)

    Science.gov (United States)

    Malik, S. K.; Umarji, A. M.; Shenoy, G. K.; Aldred, T. A.; Niarchos, D. G.

    1985-04-01

    The RRh3B2 (R=La to Gd) compounds form in the hexagonal CeCo3B2-type structure. The La containing compound in this series has been reported to be a superconductor while CeRh3B2 orders magnetically with Ce being in a nearly 3+ state. The magnetic ordering in CeRh3B2 is presumed to arise from the hybridization of nearly localized 4f electrons of Ce with sp conduction electrons or the 4d band of Rh or from the hybridization of the two. We have examined the magnetic behavior of the system Ce1-xLaxRh3B2 (x=0.0, 0.2, 0.4, 0.6, and 0.8). All these compounds (except LaRh3B2) also order magnetically. The Curie temperature decreases from about 115 K for pure Ce compound to 2.3 K for Ce0.2La0.8Rh3B2. The magnetic moments per formula unit remains nearly constant for 03B2 for x ranging from 0.0 to 0.8. The La-rich side is being investigated for the occurrence of superconductivity. Details will be published elsewhere.

  19. Data of evolutionary structure change: 1DTHB-3B8ZB [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available entryIDChain>1DTHB NGFYR----SLNIH >HHH ---- E...dbID>3B8Z B 3B8ZB NRLYSHASIENHIR ...>HHHH GGG EEEucture> ATOM 1942 CA ASN B 299 2...n> 1DTHB EIWSN-EDQIN ...>EE - > ATOM 2052 CA GLU B 57 6.491 46.203 49.509 1.0

  20. Dynamic transition of Dnmt3b expression in mouse pre- and early post-implantation embryos.

    Science.gov (United States)

    Hirasawa, Ryutaro; Sasaki, Hiroyuki

    2009-01-01

    The de novo DNA methyltransferases, Dnmt3a and Dnmt3b, are responsible for the creation of DNA methylation patterns in mouse development. Dnmt3b is more highly expressed in early developmental stages than Dnmt3a, and is thought to have an important role in the epigenetic gene regulation during early embryogenesis. Previous reports suggest that Dnmt3b is expressed preferentially in the embryonic lineage, but less in the extra-embryonic lineage, in early post-implantation embryos. However, it is unclear when this lineage-specific differential expression is established. Here we demonstrate that Dnmt3b shows a dynamic expression change during pre- and early post-implantation development. Contrary to the expectation, Dnmt3b is preferentially expressed in the trophectoderm rather than the inner cell mass at the mid blastocyst stage. Subsequently, the spatial Dnmt3b expression gradually changes during pre- and early post-implantation development, and finally Dnmt3b expression is settled in the embryonic lineage at the epiblast stage. The findings are consistent with the role for Dnmt3b in cell-lineage specification and the creation of lineage-specific DNA methylation patterns.

  1. A new phenanthro [2,3-b] furan from Pleione bulbocodioides

    Institute of Scientific and Technical Information of China (English)

    Xin Qiao Liu; Wen Yuan Gao; Yuan Qiang Guo; Tie Jun Zhang; Lu Lu Yan

    2007-01-01

    A novel phenanthro [2,3-b] furan 1, named (3-hydroxy-9-(4′-hydroxy-3′-methoxyphenyl)-11-methoxy-5,6,9,10-tetrahydrophenanthro [2,3-b] furan-10-yl) methyl acetate, and two known phenolic compounds were isolated from the tubers of Pleione bulbocodioides (Franch.) Rolfe. Their structures were elucidated by spectroscopic methods.

  2. Encyclopedia of electrochemistry. Vol. 5. Electrochemical engineering

    Energy Technology Data Exchange (ETDEWEB)

    Bard, A.J. [Texas Univ., Austin, TX (United States). Dept. of Chemistry; Stratmann, M. [Max-Planck-Institut fuer Eisenforschung GmbH, Duesseldorf (Germany); Macdonald, D. [Pennsylvania State Univ., University Park, PA (United States). Center for Electrochemical Science and Engineering; Schmuki, P. (eds.) [Erlangen-Nuernberg Univ., Erlangen (Germany). Dept. of Material Science

    2007-07-01

    This volume contains the following chapters: 1. Technical scale of electrochemistry; 2. Electrocatalysis; 3. Electrochemical composition; 4. Electrowinning of metals; 5. Electrowinning of other inorganic compounds; 6. Electrochemistry applied to organic synthesis: Principles and main achievements; 7. Batteries: Basic principles, technologies, and modeling; 8. Fuel cells; 9. The electrochemistry of nuclear reactor coolant circuits; 10. Electrochemical techniques for studying high-temperature subcritical and supercritical aqueous solutions; 11. Electrochemistry of silicon etching; 12. Electrochemical machining; 13. Environmental chemistry.

  3. Organizations and Strategies in Astronomy Vol. 7

    Science.gov (United States)

    Heck, Andre

    2006-12-01

    This book is the seventh volume under the title Organizations and Strategies in Astronomy (OSA). The OSA series covers a large range of fields and themes: in practice, one could say that all aspects of astronomy-related life and environment are considered in the spirit of sharing specific expertise and lessons learned. The chapters of this book are dealing with socio-dynamical aspects of the astronomy (and related space sciences) community: characteristics of organizations, strategies for development, operational techniques, observing practicalities, journal and magazine profiles, public outreach, publication studies, relationships with the media, research communication, series of conferences, evaluation and selection procedures, research indicators, national specificities, contemporary history, and so on. The experts contributing to this volume have done their best to write in a way understandable to readers not necessarily hyperspecialized in astronomy while providing specific detailed information and sometimes enlightening 'lessons learned' sections. The book concludes with an updated bibliography of publications related to socio-astronomy and to the interactions of the astronomy community with the society at large. This volume will be most usefully read by researchers, editors, publishers, librarians, sociologists of science, research planners and strategists, project managers, public-relations officers, plus those in charge of astronomy-related organizations, as well as by students aiming at a career in astronomy or related space sciences.

  4. IJIMAI Editor's Note - Vol. 4 Issue 5

    Directory of Open Access Journals (Sweden)

    Jose Luis Cebrian Carretero

    2017-08-01

    Full Text Available Although Medicine has always been considered a Health Science, today it is not possible to obviate its relationship with other disciplines as Humanities and Basic Sciences. Doctors from everywhere and everyday work with the most sophisticated technology are trying to make their profession more accurate and precise, taking in consideration, at the same time, the human part of their daily labour. In this volume of the Journal, we will try to explore the relation between different medical specialities, basic science and engineering. In fact, modern Medicine requires the participation of these professionals who are involved with doctors in multidisciplinary teams. In this sense, Medical Engineering, is a new degree that is offered in a vast number of Universities along the world. This relationship between Medicine and Sciences can be found in any medical speciality so that, our aim in this volume, is to show different examples of doctors working together with other scientifics in any area of Medical sciences.The volume consists on twelve papers. Each paper explores a particular area of this multidisciplinary approach.

  5. Volumes anestésicos efetivos no bloqueio do nervo isquiático: comparação entre as abordagens parassacral e infraglútea-arabiceptal com bupivacaína a 0,5% com adrenalina e ropivacaína a 0,5% Volúmenes anestésicos efectivos en el bloqueo del nervio isquiático: comparación entre los abordajes parasacral e infraglúteo-parabicipital con bupivacaína a 0,5% con adrenalina y ropivacaína a 0,5% Effective anesthetic volumes in sciatic nerve block: comparison between the parasacral and infragluteal-parabiceps approaches with 0.5% bupivacaine with adrenaline and 0.5% ropivacaine

    Directory of Open Access Journals (Sweden)

    Pablo Escovedo Helayel

    2009-10-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: O volume e a massa das soluções de anestésico local (AL influenciam a taxa de sucesso dos bloqueios periféricos. Desta forma, o objetivo principal do estudo foi determinar os volumes de anestésico local para o bloqueio do nervo isquiático (BNI nas abordagens parassacral e infraglútea-parabiceptal. MÉTODO: Cento e um pacientes foram alocados aleatoriamente em 4 grupos e submetidos ao BNI nas abordagens infraglútea-parabiceptal ou parassacral, utilizando ropivacaína a 0,5% ou bupivacaína a 0,5% com adrenalina 5 µg.mL-1. Sucesso foi definido como bloqueio sensitivo e motor completo do nervo isquiático 30 minutos após a injeção do AL. Os volumes foram calculados pelo método up-and-down. RESULTADOS: Na abordagem parassacral o volume efetivo médio da ropivacaína foi 17,6 mL (IC 95%: 14,9 - 20,8 e da bupivacaína 16,4 mL (IC 95%: 12,3 - 21,9. Na abordagem infraglútea-parabiceptal o volume efetivo médio da ropivacaína foi 21,8 mL (IC 95%: 18,7 - 25,5 e bupivacaína 20,4 mL (IC 95%: 18,6 - 22,5. Volumes foram significativamente menores (p JUSTIFICATIVA Y OBJETIVOS: El volumen y la masa de las soluciones de anestésico local (AL, influyen en la tasa de éxito de los bloqueos periféricos. Así, el objetivo principal del estudio fue determinar los volúmenes de anestésico local para el bloqueo del nervio isquiático (BNI en los abordajes parasacral e infraglúteoparabicipital. MÉTODO: Ciento y un pacientes se ubicaron aleatoriamente en 4 grupos y fueron sometidos al BNI para los abordajes infraglúteoparabicipital o parasacral, utilizando ropivacaína a 0,5% o bupivacaína a 0,5% con adrenalina 5 µg.mL-1. El éxito se definió como bloqueo sensitivo y motor completo del nervio isquiático 30 minutos después de la inyección del AL. Los volúmenes se calcularon a través del método up-and-down. RESULTADOS: En el abordaje parasacral, el volumen efectivo promedio de la ropivacaína fue de 17,6 mL (IC 95

  6. Cerebrospinal fluid-derived Semaphorin3B orients neuroepithelial cell divisions in the apicobasal axis.

    Science.gov (United States)

    Arbeille, Elise; Reynaud, Florie; Sanyas, Isabelle; Bozon, Muriel; Kindbeiter, Karine; Causeret, Frédéric; Pierani, Alessandra; Falk, Julien; Moret, Frédéric; Castellani, Valérie

    2015-02-27

    The spatial orientation of cell divisions is fundamental for tissue architecture and homeostasis. Here we analysed neuroepithelial progenitors in the developing mouse spinal cord to determine whether extracellular signals orient the mitotic spindle. We report that Semaphorin3B (Sema3B) released from the floor plate and the nascent choroid plexus in the cerebrospinal fluid (CSF) controls progenitor division orientation. Delivery of exogenous Sema3B to neural progenitors after neural tube opening in living embryos promotes planar orientation of their division. Preventing progenitor access to cues present in the CSF by genetically engineered canal obstruction affects the proportion of planar and oblique divisions. Sema3B knockout phenocopies the loss of progenitor access to the CSF. Sema3B binds to the apical surface of mitotic progenitors and exerts its effect via Neuropilin receptors, GSK3 activation and subsequent inhibition of the microtubule stabilizer CRMP2. Thus, extrinsic control mediated by the Semaphorin signalling orients progenitor divisions in neurogenic zones.

  7. APOBEC3B-Mediated Cytidine Deamination Is Required for Estrogen Receptor Action in Breast Cancer

    Directory of Open Access Journals (Sweden)

    Manikandan Periyasamy

    2015-10-01

    Full Text Available Estrogen receptor α (ERα is the key transcriptional driver in a large proportion of breast cancers. We report that APOBEC3B (A3B is required for regulation of gene expression by ER and acts by causing C-to-U deamination at ER binding regions. We show that these C-to-U changes lead to the generation of DNA strand breaks through activation of base excision repair (BER and to repair by non-homologous end-joining (NHEJ pathways. We provide evidence that transient cytidine deamination by A3B aids chromatin modification and remodelling at the regulatory regions of ER target genes that promotes their expression. A3B expression is associated with poor patient survival in ER+ breast cancer, reinforcing the physiological significance of A3B for ER action.

  8. 3D-QSAR studies on UDP-glucuronosyltransferase 2B7 substrates using the pharmacophore and VolSurf approaches.

    Science.gov (United States)

    Ako, Roland; Dong, Dong; Wu, Baojian

    2012-09-01

    UDP-glucuronosyltransferase 2B7 (UGT2B7) is an important enzyme responsible for clearance of many drugs. Here, we report two 3D quantitative structure-activity relationship (QSAR) models for UGT2B7 using the pharmacophore and VolSurf approach, respectively. The dataset included 53 structurally diverse UGT2B7 substrates, 36 of which were used for the training set and 17 of which for the external test set. Pharmacophore-based 3D-QSAR model (or hypothesis) was developed using the Discovery Studio program. A user-defined "glucuronidation site" feature was forcefully included in a pharmacophore hypothesis. VolSurf-based 3D-QSAR model was generated using the VolSurf program. This involves calculation of VolSurf descriptors, variable selection with the FFD algorithm, and partial least squares (PLS) analyses. The best pharmacophore model (r(2) = 0.736) consists of one glucuronidation site, one hydrogen bond acceptor, and three hydrophobic regions. Using this model, K(m) values for 14 of 17 test substrates were predicted within one log unit. The yielded VolSurf (PLS) model with two components shows statistical significance in both fitting and internal predicting (r(2) = 0.866, q(2) = 0.728). Further, the K(m) values for all test substrates were predicted within one log unit. In addition, the VolSurf model reveals an overlay of chemical features influencing the enzyme-substrate binding. Those include molecular size and shape, integy moments, capacity factors, best volumes of DRY probe, H-bonding, and log P. In conclusion, the pharmacophore and VolSurf approaches are successfully utilized to establish predictive models for UGT2B7. The derived models should be an efficient tool for high throughput prediction of UGT2B7 metabolism.

  9. Delta DNMT3B variants regulate DNA methylation in a promoter-specific manner.

    Science.gov (United States)

    Wang, Jie; Bhutani, Manisha; Pathak, Ashutosh K; Lang, Wenhua; Ren, Hening; Jelinek, Jaroslav; He, Rong; Shen, Lanlan; Issa, Jean-Pierre; Mao, Li

    2007-11-15

    DNA methyltransferase 3B (DNMT3B) is critical in de novo DNA methylation during development and tumorigenesis. We recently reported the identification of a DNMT3B subfamily, DeltaDNMT3B, which contains at least seven variants, resulting from alternative pre-mRNA splicing. DeltaDNMT3Bs are the predominant expression forms of DNMT3B in human lung cancer. A strong correlation was observed between the promoter methylation of RASSF1A gene but not p16 gene (both frequently inactivated by promoter methylation in lung cancer) and expression of DeltaDNMT3B4 in primary lung cancer, suggesting a role of DeltaDNMT3B in regulating promoter-specific methylation of common tumor suppressor genes in tumorigenesis. In this report, we provide first experimental evidence showing a direct involvement of DeltaDNMT3B4 in regulating RASSF1A promoter methylation in human lung cancer cells. Knockdown of DeltaDNMT3B4 expression by small interfering RNA resulted in a rapid demethylation of RASSF1A promoter and reexpression of RASSF1A mRNA but had no effect on p16 promoter in the lung cancer cells. Conversely, normal bronchial epithelial cells with stably transfected DeltaDNMT3B4 gained an increased DNA methylation in RASSF1A promoter but not p16 promoter. We conclude that promoter DNA methylation can be differentially regulated and DeltaDNMT3Bs are involved in regulation of such promoter-specific de novo DNA methylation.

  10. Characterization of the relationship between APOBEC3B deletion and ACE Alu insertion.

    Directory of Open Access Journals (Sweden)

    Kang Wang

    Full Text Available The insertion/deletion (I/D polymorphism of the angiotensin converting enzyme (ACE, commonly associated with many diseases, is believed to have affected human adaptation to environmental changes during the out-of-Africa expansion. APOBEC3B (A3B, a member of the cytidine deaminase family APOBEC3s, also exhibits a variable gene insertion/deletion polymorphism across world populations. Using data available from published reports, we examined the global geographic distribution of ACE and A3B genotypes. In tracking the modern human dispersal routes of these two genes, we found that the variation trends of the two I/D polymorphisms were directly correlated. We observed that the frequencies of ACE insertion and A3B deletion rose in parallel along the expansion route. To investigate the presence of a correlation between the two polymorphisms and the effect of their interaction on human health, we analyzed 1199 unrelated Chinese adults to determine their genotypes and other important clinical characteristics. We discovered a significant difference between the ACE genotype/allele distribution in the A3B DD and A3B II/ID groups (P = 0.045 and 0.015, respectively, indicating that the ACE Alu I allele frequency in the former group was higher than in the latter group. No specific clinical phenotype could be associated with the interaction between the ACE and A3B I/D polymorphisms. A3B has been identified as a powerful inhibitor of Alu retrotransposition, and primate A3 genes have undergone strong positive selection (and expansion for restricting the mobility of endogenous retrotransposons during evolution. Based on these findings, we suggest that the ACE Alu insertion was enabled (facilitated by the A3B deletion and that functional loss of A3B provided an opportunity for enhanced human adaptability and survival in response to the environmental and climate challenges arising during the migration from Africa.

  11. Encyclopedia of electrochemistry. Vol. 10. Modified electrodes

    Energy Technology Data Exchange (ETDEWEB)

    Bard, A.J. [Texas Univ., Austin, TX (United States). Dept. of Chemistry; Stratmann, M. [Max-Planck-Institut fuer Eisenforschung GmbH, Duesseldorf (Germany); Rubinstein, I. [Weizmann Institute of Science, Rehovot (Israel). Dept. of Materials and Interfaces; Fujihira, Masamichi [Tokyo Institute of Technology, Yokohama (Japan). Dept. of Biomolecular Engineering; Rusling, J.F. (eds.) [Connecticut Univ., Storrs, CT (United States). Dept. of Chemistry, U-60]|[Connecticut Univ., Storrs, CT (United States). Dept. of Pharmacology

    2007-07-01

    This volume contains the following topics: 1. Preparation of monolayer modified electrodes; 2. Layer-by-layer assemblies of thin films on electrodes; 3. Epitaxial electrochemical growth; 5. Other films; 6. Ex-situ methods; 7. In-situ methods; 8. Electron transfer; 9. Charge transport in polymer-modified electrodes; 10. Electrochemical reactions on modified electrodes; 11. Redox-active dendrimers in solution and as films on surfaces; 12. Electrochemical formation of organic thin films; 13. Electron transfer and transport in ordered enzyme layers.

  12. From Editor vol 11, No.3

    OpenAIRE

    Ugur Demiray

    2010-01-01

    Greetings Dear readers of TOJDE,TOJDE is appeared on your screen now as Volume 11, Number: 3. In this issue it is published 3 notes for Editor, 12 articles, 2 book reviews. And this time, 32 authors from 10 different countries are placed. These published articles are from Barbados, Ghana, Iran, Malaysia, Pakistan, Spain, Turkey, United Kingdom, UAE and USA.“Developing and Validation A Usability Evaluation Tools For Distance Education Websites:Persian Version” has sent as Notes for editor se...

  13. From Editor vol 11, No.4

    OpenAIRE

    Ugur Demiray

    2010-01-01

    Greetings Dear readers of TOJDE,TOJDE is appeared on your screen now as Volume 11, Number: 4. In this issue it is published 4 notes for Editor, 12 articles, 2 book and one conference reviews. And this time, 27 authors from 10 different countries are placed. These published articles are from Bangldesh, Greece, India, Israel, Malaysia, Nigeria, Portugal, Singapore, Turkey and USA.The first Notes for editor arrived from USA, written by Kevin YEE and Jace HARGIS. They focused on PREZI: A Differen...

  14. DNA methyltransferase 3B (DNMT3B -579G>T) promotor polymorphism and the susceptibility to pediatric immune thrombocytopenic purpura in Egypt.

    Science.gov (United States)

    Khorshied, Mervat Mamdooh; El-Ghamrawy, Mona Kamal

    2012-12-10

    Idiopathic thrombocytopenic purpura (ITP) is an autoimmune disease characterized by increased platelet destruction. Although the etiology of ITP remains unclear, it is accepted that both environmental and genetic factors play an important role in the development of the disease. The present study aimed at exploring a novel molecular determinant that may influence the susceptibility and course of ITP in Egyptian children. To achieve our aim, genotyping of DNMT3B -579G>T promotor polymorphism by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assay. The current study was conducted on 140 ITP patients and 150 age and gender matched healthy controls. The results obtained revealed that DNMT3B -579 TT homotype was significantly higher in ITP patients and conferred almost three fold increased risk of ITP (OR=3.16, 95%CI=1.73-5.79). There was no statistically significant difference between ITP patients with wild or mutant genotypes as regards their clinical or laboratory data. Furthermore, there was no statistical difference in the distribution of DNMT3B -579G>T genotypes between acute and chronic ITP patients. In conclusion, DNMT3B -579G>T promotor polymorphism represents a novel genetic risk factor for ITP but not a predictor for tendency to chronicity in pediatric ITP in Egypt.

  15. Deficiency for the ubiquitin ligase UBE3B in a blepharophimosis-ptosis-intellectual-disability syndrome.

    Science.gov (United States)

    Basel-Vanagaite, Lina; Dallapiccola, Bruno; Ramirez-Solis, Ramiro; Segref, Alexandra; Thiele, Holger; Edwards, Andrew; Arends, Mark J; Miró, Xavier; White, Jacqueline K; Désir, Julie; Abramowicz, Marc; Dentici, Maria Lisa; Lepri, Francesca; Hofmann, Kay; Har-Zahav, Adi; Ryder, Edward; Karp, Natasha A; Estabel, Jeanne; Gerdin, Anna-Karin B; Podrini, Christine; Ingham, Neil J; Altmüller, Janine; Nürnberg, Gudrun; Frommolt, Peter; Abdelhak, Sonia; Pasmanik-Chor, Metsada; Konen, Osnat; Kelley, Richard I; Shohat, Mordechai; Nürnberg, Peter; Flint, Jonathan; Steel, Karen P; Hoppe, Thorsten; Kubisch, Christian; Adams, David J; Borck, Guntram

    2012-12-07

    Ubiquitination plays a crucial role in neurodevelopment as exemplified by Angelman syndrome, which is caused by genetic alterations of the ubiquitin ligase-encoding UBE3A gene. Although the function of UBE3A has been widely studied, little is known about its paralog UBE3B. By using exome and capillary sequencing, we here identify biallelic UBE3B mutations in four patients from three unrelated families presenting an autosomal-recessive blepharophimosis-ptosis-intellectual-disability syndrome characterized by developmental delay, growth retardation with a small head circumference, facial dysmorphisms, and low cholesterol levels. UBE3B encodes an uncharacterized E3 ubiquitin ligase. The identified UBE3B variants include one frameshift and two splice-site mutations as well as a missense substitution affecting the highly conserved HECT domain. Disruption of mouse Ube3b leads to reduced viability and recapitulates key aspects of the human disorder, such as reduced weight and brain size and a downregulation of cholesterol synthesis. We establish that the probable Caenorhabditis elegans ortholog of UBE3B, oxi-1, functions in the ubiquitin/proteasome system in vivo and is especially required under oxidative stress conditions. Our data reveal the pleiotropic effects of UBE3B deficiency and reinforce the physiological importance of ubiquitination in neuronal development and function in mammals.

  16. The Role of PDE3B Phosphorylation in the Inhibition of Lipolysis by Insulin.

    Science.gov (United States)

    DiPilato, Lisa M; Ahmad, Faiyaz; Harms, Matthew; Seale, Patrick; Manganiello, Vincent; Birnbaum, Morris J

    2015-08-01

    Inhibition of adipocyte lipolysis by insulin is important for whole-body energy homeostasis; its disruption has been implicated as contributing to the development of insulin resistance and type 2 diabetes mellitus. The main target of the antilipolytic action of insulin is believed to be phosphodiesterase 3B (PDE3B), whose phosphorylation by Akt leads to accelerated degradation of the prolipolytic second messenger cyclic AMP (cAMP). To test this hypothesis genetically, brown adipocytes lacking PDE3B were examined for their regulation of lipolysis. In Pde3b knockout (KO) adipocytes, insulin was unable to suppress β-adrenergic receptor-stimulated glycerol release. Reexpressing wild-type PDE3B in KO adipocytes fully rescued the action of insulin against lipolysis. Surprisingly, a mutant form of PDE3B that ablates the major Akt phosphorylation site, murine S273, also restored the ability of insulin to suppress lipolysis. Taken together, these data suggest that phosphorylation of PDE3B by Akt is not required for insulin to suppress adipocyte lipolysis. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  17. Assay development for the discovery of semaphorin 3B inducing agents from natural product sources.

    Science.gov (United States)

    Yong, Yeonjoong; Pan, Li; Ren, Yulin; Fatima, Nighat; Ahmed, Safia; Chang, Leng Chee; Zhang, Xiaoli; Kinghorn, A Douglas; Swanson, Steven M; Carcache de Blanco, Esperanza J

    2014-10-01

    Semaphorins are a class of membrane-bound and secreted proteins. They have been found to regulate basic cell functions such as axonal growth cone guidance and recent studies have focused on their effect on tumor progression. Semaphorin 3B (Sema3B) particularly is a secreted protein that has been known to modulate proliferation and apoptosis, processes that are critical for tumor progression and development. In spite of its importance, there is yet no high-throughput screening assay available to detect or quantify the expression of Sema3B for natural product anticancer drug discovery purposes. Therefore, the development of a new high-throughput bioassay for the discovery of Sema3B inducing agents from natural product sources is described herein. A wide variety of pure compounds and extracts from plants and microorganisms has been found suitable for screening using this Sema3B assay to detect and quantify the effect of Sema3B inducing agents and thereby identify new selective bioactive Sema3B lead compounds for anticancer drug discovery and development. Also, this new bioassay procedure is based on a high-throughput platform using an enzyme-linked immunosorbent assay that involves the optimization of sensitivity and selectivity levels as well as accuracy, reproducibility, robustness, and cost effectiveness.

  18. DNMT3B gene amplification predicts resistance to DNA demethylating drugs.

    Science.gov (United States)

    Simó-Riudalbas, Laia; Melo, Sónia A; Esteller, Manel

    2011-07-01

    Disruption of the DNA methylation landscape is one of the most common features of human tumors. However, genetic alterations of DNA methyltransferases (DNMTs) have not been described in carcinogenesis. Herein, we show that pancreatic and breast cancer cells undergo gene amplification of the DNA methyltransferase 3B (DNMT3B). The presence of extra copies of the DNMT3B gene is linked to higher levels of the corresponding mRNA and protein. Most importantly, the elevated gene dosage of DNMT3B is associated with increased resistance to the growth-inhibitory effect mediated by DNA demethylating agents. In particular, cancer cells harboring DNMT3B gene amplification are less sensitive to the decrease in cell viability caused by 5-azacytidine (Vidaza), 5-aza-2-deoxycytidine (Decitabine), and SGI-1027. Overall, the data confirm DNMT3B as a bona fide oncogene in human cancer and support the incorporation of the DNMT3B copy number assay into current clinical trials assessing the efficacy of DNA demethylating drugs in solid tumors.

  19. Association of the DNMT3B polymorphism with colorectal adenomatous polyps and adenocarcinoma.

    Science.gov (United States)

    Guo, Xiaoqing; Zhang, Liwei; Wu, Mingli; Wang, Na; Liu, Yanfeng; Er, Limian; Wang, Shunping; Gao, Yang; Yu, Weifang; Xue, Hui; Xu, Zhibin; Wang, Shijie

    2010-01-01

    DNMT3B is an important enzyme to modulate the methylation status in mammalian cells. The aim of this study is to investigate the correlation of the DNMT3B G39179T polymorphism with the susceptibilities of colorectal adenomatous polyps and adenocarcinoma. This case-control study included 146 colorectal adenomatous polyps, 170 colorectal adenocarcinoma patients, and 157 normal controls. DNMT3B polymorphism was analyzed by polymerase chain reaction-restriction fragment length polymorphism analysis. Family history of colorectal cancer significantly increases the risk of developing colorectal adenomatous polyps and adenocarcinoma. The genotype frequency of DNMT3B polymorphism (T/T and G/T + G/G) in adenocarcinoma patients was significantly different from that in controls (P value = 0.01). Compared with DNMT3B T/T genotype, the G allelotype (G/T + G/G genotype) had lower risk to develop colorectal adenocarcinoma (OR = 0.50, 95% CI = 0.29-0.87); while there was no significant difference between the colorectal adenomatous polyps patients and controls (OR = 0.63, 95% CI = 0.37-1.09), although descending tendency could be found in this polyps group. In the stratification analysis, a significant association was confined to subgroups of age DNMT3B G39179T SNP in different ethnics. DNMT3B G39179T SNP may be a potential genetic susceptibility factor for adenocarcinoma of the colon, especially in younger Chinese Han non-drinker men.

  20. DNMT3B inhibits the re-expression of genes associated with induced pluripotency.

    Science.gov (United States)

    Wongtrakoongate, Patompon; Li, Jianliang; Andrews, Peter W

    2014-02-15

    DNMT3B is a de novo DNA methyltransferase that is highly expressed in mouse and human embryonic stem (ES) cells and has been shown to be essential for differentiation of mouse ES cells toward different lineages. In the present study, we found that DNMT3B is rapidly down-regulated in human ES cells during retinoic acid (RA)-induced differentiation compared with DNMT3A2, which is also highly expressed in ES cells. Silencing of DNMT3B in human ES cells by an inducible shRNAi system leads to a reduction of clonal ability of the stem cells, while expression of OCT4 and NANOG is unchanged. By contrast, the germline-specific genes VASA and SCP3 and the surface antigen BE12 are down regulated following DNMT3B knockdown. Upon retinoic acid-induced differentiation, we found that depletion of DNMT3B leads to a decrease in expression of the surface antigen A2B5 and of neural tube-associated genes PAX7 and BRN3A. Consistent with its importance in stem cell differentiation, we observed that silencing of DNMT3B facilitates the generation of cells that bear the hallmarks of pluripotency. Our findings suggest a role of DNMT3B in controlling the differentiation of human ES cells and in the generation of iPS cells.

  1. DNMT1 and DNMT3B modulate distinct polycomb-mediated histone modifications in colon cancer.

    Science.gov (United States)

    Jin, Bilian; Yao, Bing; Li, Jian-Liang; Fields, C Robert; Delmas, Amber L; Liu, Chen; Robertson, Keith D

    2009-09-15

    DNA methylation patterns are established and maintained by three DNA methyltransferases (DNMT): DNMT1, DNMT3A, and DNMT3B. Although essential for development, methylation patterns are frequently disrupted in cancer and contribute directly to carcinogenesis. Recent studies linking polycomb group repression complexes (PRC1 and PRC2) to the DNMTs have begun to shed light on how methylation is targeted. We identified previously a panel of genes regulated by DNMT3B. Here, we compare these with known polycomb group targets to show that approximately 47% of DNMT3B regulated genes are also bound by PRC1 or PRC2. We chose 44 genes coregulated by DNMT3B and PRC1/PRC2 to test whether these criteria would accurately identify novel targets of epigenetic silencing in colon cancer. Using reverse transcription-PCR, bisulfite genomic sequencing, and pyrosequencing, we show that the majority of these genes are frequently silenced in colorectal cancer cell lines and primary tumors. Some of these, including HAND1, HMX2, and SIX3, repressed cell growth. Finally, we analyzed the histone code, DNMT1, DNMT3B, and PRC2 binding by chromatin immunoprecipitation at epigenetically silenced genes to reveal a novel link between DNMT3B and the mark mediated by PRC1. Taken together, these studies suggest that patterns of epigenetic modifiers and the histone code influence the propensity of a gene to become hypermethylated in cancer and that DNMT3B plays an important role in regulating PRC1 function.

  2. Inactive DNMT3B splice variants modulate de novo DNA methylation.

    Science.gov (United States)

    Gordon, Catherine A; Hartono, Stella R; Chédin, Frédéric

    2013-01-01

    Inactive DNA methyltransferase (DNMT) 3B splice isoforms are associated with changes in DNA methylation, yet the mechanisms by which they act remain largely unknown. Using biochemical and cell culture assays, we show here that the inactive DNMT3B3 and DNMT3B4 isoforms bind to and regulate the activity of catalytically competent DNMT3A or DNMT3B molecules. DNMT3B3 modestly stimulated the de novo methylation activity of DNMT3A and also counteracted the stimulatory effects of DNMT3L, therefore leading to subtle and contrasting effects on activity. DNMT3B4, by contrast, significantly inhibited de novo DNA methylation by active DNMT3 molecules, most likely due to its ability to reduce the DNA binding affinity of co-complexes, thereby sequestering them away from their substrate. Immunocytochemistry experiments revealed that in addition to their effects on the intrinsic catalytic function of active DNMT3 enzymes, DNMT3B3 and DNMT34 drive distinct types of chromatin compaction and patterns of histone 3 lysine 9 tri-methylation (H3K9me3) deposition. Our findings suggest that regulation of active DNMT3 members through the formation of co-complexes with inactive DNMT3 variants is a general mechanism by which DNMT3 variants function. This may account for some of the changes in DNA methylation patterns observed during development and disease.

  3. Inactive DNMT3B splice variants modulate de novo DNA methylation.

    Directory of Open Access Journals (Sweden)

    Catherine A Gordon

    Full Text Available Inactive DNA methyltransferase (DNMT 3B splice isoforms are associated with changes in DNA methylation, yet the mechanisms by which they act remain largely unknown. Using biochemical and cell culture assays, we show here that the inactive DNMT3B3 and DNMT3B4 isoforms bind to and regulate the activity of catalytically competent DNMT3A or DNMT3B molecules. DNMT3B3 modestly stimulated the de novo methylation activity of DNMT3A and also counteracted the stimulatory effects of DNMT3L, therefore leading to subtle and contrasting effects on activity. DNMT3B4, by contrast, significantly inhibited de novo DNA methylation by active DNMT3 molecules, most likely due to its ability to reduce the DNA binding affinity of co-complexes, thereby sequestering them away from their substrate. Immunocytochemistry experiments revealed that in addition to their effects on the intrinsic catalytic function of active DNMT3 enzymes, DNMT3B3 and DNMT34 drive distinct types of chromatin compaction and patterns of histone 3 lysine 9 tri-methylation (H3K9me3 deposition. Our findings suggest that regulation of active DNMT3 members through the formation of co-complexes with inactive DNMT3 variants is a general mechanism by which DNMT3 variants function. This may account for some of the changes in DNA methylation patterns observed during development and disease.

  4. An essential role for DNA methyltransferase DNMT3B in cancer cell survival.

    Science.gov (United States)

    Beaulieu, Normand; Morin, Steves; Chute, Ian C; Robert, Marie-France; Nguyen, Hannah; MacLeod, A Robert

    2002-08-02

    Abnormal methylation and associated silencing of tumor suppressor genes is a common feature of many types of cancers. The observation of persistent methylation in human cancer cells lacking the maintenance methyltransferase DNMT1 suggests the involvement of other DNA methyltransferases in gene silencing in cancer. To test this hypothesis, we have evaluated methylation and gene expression in cancer cells specifically depleted of DNMT3A or DNMT3B, de novo methyltransferases that are expressed in adult tissues. Here we have shown that depletion of DNMT3B, but not DNMT3A, induced apoptosis of human cancer cells but not normal cells. DNMT3B depletion reactivated methylation-silenced gene expression but did not induce global or juxtacentromeric satellite demethylation as did specific depletion of DNMT1. Furthermore, the effect of DNMT3B depletion was rescued by exogenous expression of either of the splice variants DNMT3B2 or DNMT3B3 but not DNMT1. These results indicate that DNMT3B has significant site selectivity that is distinct from DNMT1, regulates aberrant gene silencing, and is essential for cancer cell survival.

  5. Intelligent autonomous systems 12. Vol. 2. Proceedings

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Sukhan [Sungkyunkwan Univ., Gyeonggi-Do (Korea, Republic of). College of Information and Communication Engineering; Yoon, Kwang-Joon [Konkuk Univ., Seoul (Korea, Republic of); Cho, Hyungsuck [Daegu Gyeongbuk Institute of Science and Technology, Daegu (Korea, Republic of); Lee, Jangmyung (eds.) [Pusan National Univ. (Korea, Republic of). Dept. of Electronics Engineering

    2013-02-01

    Recent research in Intelligent and Autonomous Systems. Volume 2 of the proceedings of the 12th International Conference IAS-12, held June 26-29, 2012, jeju Island, Korea. Written by leading experts in the field. Intelligent autonomous systems are emerged as a key enabler for the creation of a new paradigm of services to humankind, as seen by the recent advancement of autonomous cars licensed for driving in our streets, of unmanned aerial and underwater vehicles carrying out hazardous tasks on-site, and of space robots engaged in scientific as well as operational missions, to list only a few. This book aims at serving the researchers and practitioners in related fields with a timely dissemination of the recent progress on intelligent autonomous systems, based on a collection of papers presented at the 12th International Conference on Intelligent Autonomous Systems, held in Jeju, Korea, June 26-29, 2012. With the theme of ''Intelligence and Autonomy for the Service to Humankind, the conference has covered such diverse areas as autonomous ground, aerial, and underwater vehicles, intelligent transportation systems, personal/domestic service robots, professional service robots for surgery/rehabilitation, rescue/security and space applications, and intelligent autonomous systems for manufacturing and healthcare. This volume 2 includes contributions devoted to Service Robotics and Human-Robot Interaction and Autonomous Multi-Agent Systems and Life Engineering.

  6. Elevated APOBEC3B correlates with poor outcomes for estrogen-receptor-positive breast cancers.

    Science.gov (United States)

    Sieuwerts, Anieta M; Willis, Scooter; Burns, Michael B; Look, Maxime P; Meijer-Van Gelder, Marion E; Schlicker, Andreas; Heideman, Marinus R; Jacobs, Heinz; Wessels, Lodewyk; Leyland-Jones, Brian; Gray, Kathryn P; Foekens, John A; Harris, Reuben S; Martens, John W M

    2014-12-01

    Recent observations connected DNA cytosine deaminase APOBEC3B to the genetic evolution of breast cancer. We addressed whether APOBEC3B is associated with breast cancer clinical outcomes. APOBEC3B messenger RNA (mRNA) levels were related in 1,491 primary breast cancers to disease-free (DFS), metastasis-free (MFS), and overall survival (OS). For independent validation, APOBEC3B mRNA expression was associated with patient outcome data in five additional cohorts (over 3,500 breast cancer cases). In univariate Cox regression analysis, increasing APOBEC3B expression as a continuous variable was associated with worse DFS, MFS, and OS (hazard ratio [HR] = 1.20, 1.21, and 1.24, respectively; all P DFS (continuous variable: HR = 1.29, P = .001; dichotomized at the median level, HR = 1.66, P = .0002). This implies that APOBEC3B is a marker of pure prognosis in ER + disease. These findings were confirmed in the analyses of five independent patient sets. In these analyses, APOBEC3B expression dichotomized at the median level was associated with adverse outcomes (METABRIC discovery and validation, 788 and 706 ER + cases, disease-specific survival (DSS), HR = 1.77 and HR = 1.77, respectively, both P DFS, HR = 1.57, P = 2.46E-04; NKI295, 181 ER + cases, DFS, HR = 1.72, P = .054; and BIG 1-98, 1,219 ER + cases, breast-cancer-free interval (BCFI), HR = 1.42, P = 0.0079). APOBEC3B is a marker of pure prognosis and poor outcomes for ER + breast cancer, which strongly suggests that genetic aberrations induced by APOBEC3B contribute to breast cancer progression.

  7. Progressive APOBEC3B mRNA expression in distant breast cancer metastases

    Science.gov (United States)

    Dalm, Simone U.; de Weerd, Vanja; Moelans, Cathy B.; ter Hoeve, Natalie; van Diest, Paul J.; Martens, John W. M.; van Deurzen, Carolien H. M.

    2017-01-01

    Background APOBEC3B was recently identified as a gain-of-function enzymatic source of mutagenesis, which may offer novel therapeutic options with molecules that specifically target this enzyme. In primary breast cancer, APOBEC3B mRNA is deregulated in a substantial proportion of cases and its expression is associated with poor prognosis. However, its expression in breast cancer metastases, which are the main causes of breast cancer-related death, remained to be elucidated. Patients and methods RNA was isolated from 55 primary breast cancers and paired metastases, including regional lymph node (N = 20) and distant metastases (N = 35). APOBEC3B mRNA levels were measured by RT-qPCR. Expression levels of the primary tumors and corresponding metastases were compared, including subgroup analysis by estrogen receptor (ER/ESR1) status. Results Overall, APOBEC3B mRNA levels of distant metastases were significantly higher as compared to the corresponding primary breast tumor (P = 0.0015), an effect that was not seen for loco-regional lymph node metastases (P = 0.23). Subgroup analysis by ER-status showed that increased APOBEC3B levels in distant metastases were restricted to metastases arising from ER-positive primary breast cancers (P = 0.002). However, regarding ER-negative primary tumors, only loco-regional lymph node metastases showed increased APOBEC3B expression when compared to the corresponding primary tumor (P = 0.028). Conclusion APOBEC3B mRNA levels are significantly higher in breast cancer metastases as compared to the corresponding ER-positive primary tumors. This suggests a potential role for APOBEC3B in luminal breast cancer progression, and consequently, a promising role for anti-APOBEC3B therapies in advanced stages of this frequent form of breast cancer. PMID:28141868

  8. Physical properties of ferroelectric superlattice A3/B3 system in electric field

    Institute of Scientific and Technical Information of China (English)

    Jiang Wei; Lo Veng-Cheong; Bai Bao-Dong

    2005-01-01

    Based on the differential operator technique, a transverse Ising model (TIM) in the effective-field theory is developed to study the physical properties of a ferroelectric superlattice A3/B3 system. The effects of an external electric field on the polarization, susceptibility and pyroelectric coefficient of the ferroelectric superlattice A3/B3 system are discussed in detail. The susceptibility of the ferroelectric superlattice A3/B3 system decreases with the increase of the electric field, implying that the polarization is weak.

  9. Preliminary Results for the Radiation Environment Observed by RD3-B3 Radiometerdosimeter Inside Bion-M # 1 Spacecraft

    Science.gov (United States)

    Dachev, Tsvetan; Tomov, Borislav; Matviichuk, Yury; Dimitrov, Plamen; Bankov, Nikolay; Petrov, Vladisloav; Shurshakov, Viacheslav; Ivanova, Olga; Häder, Donat-Peter; Lebert, Michael; Schuster, Martin; Reitz, Günter; Horneck, Gerda; Ploc, Ondrej

    2013-12-01

    Space radiation has been monitored using the P#xd0bb;3-킑3 (further is used the Latin transcription RD3-B3) spectrometer-dosimeter on board a recent space flight on the Russian recoverable satellite 킑킍OH-M No.1 (further is used the Latin transcription BIONM No. 1). The instrument was mounted inside of the satellite in pressurized volume together with biological objects and samples. RD3-B3 instrument is a battery operated version of the spare model of the R3D-B3 instrument developed and built for the ESA BIOPAN-6 facility on Foton M3 satellite flown in September 2007. Cosmic ionizing radiation has been monitored and separated in 256 deposited energy spectra, which were further used for determination of the absorbed dose rate and flux. The report summarizes the first results for the Earth radiation environment at the altitude (253-585 km) of the BION-M No.1 spacecraft.

  10. Handbook of nuclear engineering: vol 1: nuclear engineering fundamentals; vol 2: reactor design; vol 3: reactor analysis; vol 4: reactors of waste disposal and safeguards

    CERN Document Server

    2013-01-01

    The Handbook of Nuclear Engineering is an authoritative compilation of information regarding methods and data used in all phases of nuclear engineering. Addressing nuclear engineers and scientists at all academic levels, this five volume set provides the latest findings in nuclear data and experimental techniques, reactor physics, kinetics, dynamics and control. Readers will also find a detailed description of data assimilation, model validation and calibration, sensitivity and uncertainty analysis, fuel management and cycles, nuclear reactor types and radiation shielding. A discussion of radioactive waste disposal, safeguards and non-proliferation, and fuel processing with partitioning and transmutation is also included. As nuclear technology becomes an important resource of non-polluting sustainable energy in the future, The Handbook of Nuclear Engineering is an excellent reference for practicing engineers, researchers and professionals.

  11. The theory of the firm. Vol. 2

    DEFF Research Database (Denmark)

    Firms have for a long time been part of the explanatory set-up of economics. However, it is only recently that economists have felt the need for an economic theory addressing: why firms are different; why firms exist; what determines their boundaries relative to "the market"; and what determines...... their internal organization. This collection documents the rise of the modern theory of the firm during the last two to three decades. It reprints classic writings from a diversity of perspectives, including not only contractual theories of the firm, but also knowledge-based theories and theories of the firm...... as an information processor. In addition, the collection features perspectives from business strategy and business history as well as methodological and doctrinal historical perspectives. Including over 60 classic papers, these volumes collect together contributions on the theory of the firm, beginning with Ronald...

  12. Engineering Education and Management - vol.2

    CERN Document Server

    Zhang, Chunliang; International Conference on Engineering Education and Management (ICEEM2011)

    2012-01-01

    This is the proceedings of the selected papers presented at 2011 International Conference on Engineering Education and Management (ICEEM2011) held in Guangzhou, China, during November 18-20, 2011. ICEEM2011 is one of the most important conferences in the field of Engineering Education and Management and is co-organized by Guangzhou University, The University of New South Wales, Zhejiang University and Xi’an Jiaotong University. The conference aims to provide a high-level international forum for scientists, engineers, and students to present their new advances and research results in the field of Engineering Education and Management. This volume comprises 122 papers selected from over 400 papers originally submitted by universities and industrial concerns all over the world. The papers specifically cover the topics of Management Science and Engineering, Engineering Education and Training, Project/Engineering Management, and Other related topics. All of the papers were peer-reviewed by selected experts. The p...

  13. Safety of magnetic fusion facilities: Volume 2, Guidance

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-07-01

    This document provides guidance for the implementation of the requirements identified in Vol. 1 of this Standard. This guidance is intended for the managers, designers, operators, and other personnel with safety responsibilities for facilities designated as magnetic fusion facilities. While Vol. 1 is generally applicable in that requirements there apply to a wide range of fusion facilities, this volume is concerned mainly with large facilities such as the International Thermonuclear Experimental Reactor (ITER). Using a risk-based prioritization, the concepts presented here may also be applied to other magnetic fusion facilities. This volume is oriented toward regulation in the Department of Energy (DOE) environment.

  14. Technical comparison between Hythane, GNG and gasoline fueled vehicles. [Hythane = 85 vol% natural gas, 15 vol% H[sub 2

    Energy Technology Data Exchange (ETDEWEB)

    1992-05-01

    This interim report documents progress on this 2-year Alternative Fuel project, scheduled to end early 1993. Hythane is 85 vol% compressed natural gas (CNG) and 15 vol% hydrogen; it has the potential to meet or exceed the California Ultra-Low Emission Vehicle (ULEV) standard. Three USA trucks (3/4 ton pickup) were operated on single fuel (unleaded gasoline, CNG, Hythane) in Denver. The report includes emission testing, fueling facility, hazard and operability study, and a framework for a national hythane strategy.

  15. Data of evolutionary structure change: 1ADIA-1DJ3B [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1ADIA-1DJ3B 1ADI 1DJ3 A B -------GNNVVVLGTQWGDEGKGKIVDLLTERAKYVVR...KVARRGLRVGDLFDKETFAEKLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDFVMFEGAQGTLLDIDHGTYPYVTSSNTTAGG...alignment> 0 1ADI A 1ADI.../n14> 1ADI A 1ADIA

  16. Ferromagnetic enhancement in the hexagonal Ce(Rh1-xIrx)3B2 system

    Science.gov (United States)

    Hsu, S. W.; Ku, H. C.

    1988-08-01

    Electrical resistivity, magnetization, and crystallographic measurements have been carried out for the pseudoternary system Ce(Rh1-xIrx)3B2. A single-phase hexagonal CeCo3B2-type structure persists from x=0.0 up to x0.95. The ferromagnetic Curie temperature Tc is enhanced from 115 K for CeRh3B2 to 125 K for Ce(Rh0.8Ir0.2)32, then decreases slowly to 118 K at x=0.5 before decreasing sharply to 69 K at x=0.67, even though the magnetic moment per Ce ion decreases monotonically with increasing Ir substitution. The Tc of 125 K in Ce(Rh0.8Ir0.2)3B2 is the highest magnetic-ordering temperature observed so far for all Ce-based systems with nonmagnetic constituents.

  17. The impact of SF3B1 mutations in CLL on the DNA-damage response

    DEFF Research Database (Denmark)

    Te Raa, G D; Derks, I A M; Navrkalova, V;

    2015-01-01

    Mutations or deletions in TP53 or ATM are well-known determinants of poor prognosis in chronic lymphocytic leukemia (CLL), but only account for approximately 40% of chemo-resistant patients. Genome-wide sequencing has uncovered novel mutations in the splicing factor sf3b1, that were in part...... associated with ATM aberrations, suggesting functional synergy. We first performed detailed genetic analyses in a CLL cohort (n=110) containing ATM, SF3B1 and TP53 gene defects. Next, we applied a newly developed multiplex assay for p53/ATM target gene induction and measured apoptotic responses to DNA damage....... Interestingly, SF3B1 mutated samples without concurrent ATM and TP53 aberrations (sole SF3B1) displayed partially defective ATM/p53 transcriptional and apoptotic responses to various DNA-damaging regimens. In contrast, NOTCH1 or K/N-RAS mutated CLL displayed normal responses in p53/ATM target gene induction...

  18. Study of magnetic behaviour of CeRh3B2 using111Cd probe

    Science.gov (United States)

    Nyayate, M. N.; Devare, S. H.; Devare, H. G.

    1989-06-01

    Using111Cd probe nuclei, the transferred H hf( T) is measured down to 35 K in CeRh3B2 using TDPAC technique. The angle β between the EFG and the direction of magnetization is obtained to be 90°. The observed small H hf( T) may be a consequence of the reduced moment on Ce in CeRh3B2.

  19. The Role of DN-GSK3b in Mammary Tumorigenesis

    Science.gov (United States)

    2007-07-01

    Publications Marganit Farago, Isabel Dominguez, Esther Landesman-Bollag, Xin Xu, Andrea Rosner, Robert D. Cardiff, and David C. Seldin . “Kinase Inactive...Isabel Dominguez, Esther Landesman , Xin Xu, and David C. Seldin Farago, June 2005. ”Kinase inactive GSK3b promotes Wnt signaling and mammary...Esther Landesman-Bollag, and David C. Seldin . 2004 “Kinase Inactive GSK3b Promotes Wnt Signaling and Mammary Tumorigenesis” BRCA (Boston Cancer

  20. Gene-specific DNA methylation of DNMT3B and MTHFR and colorectal adenoma risk.

    Science.gov (United States)

    Ho, Vikki; Ashbury, Janet E; Taylor, Sherryl; Vanner, Stephen; King, Will D

    2015-12-01

    DNA methyltransferase 3B (DNMT3B) and methylenetetrahydrofolate reductase (MTHFR) are genes which encode enzymes critical to one-carbon metabolism. Polymorphisms in these genes have been implicated in colorectal cancer etiology; however, epigenetic modifications such as gene-specific DNA methylation also affect gene expression. DNA methylation of DNMT3B and MTHFR was quantified in blood leukocytes using Sequenom EpiTYPER® among 272 participants undergoing a screening colonoscopy. DNA methylation was quantified in 66 and 28CpG sites of DNMT3B and MTHFR respectively, and conceptualized using two approaches. First, measures representing average methylation across all CpG sites were created. Second, unsupervised principal component (PC) analysis was used to identify summary variables representing methylation around the transcription start site and in the gene-coding area for both DNMT3B and MTHFR. Logistic regression was used to compare methylation levels between participants diagnosed with colorectal adenoma(s) versus those with a normal colonoscopy via the estimation of odds ratios (ORs) and 95% confidence intervals (95% CIs) for the risk of colorectal adenomas. No association was observed between average DNA methylation of either DNMT3B or MTHFR and colorectal adenoma risk. For DNMT3B, increasing DNA methylation of CpG sites in the gene-coding area was associated with a higher risk of colorectal adenomas (OR=1.34; 95% CI: 1.01-1.79 per SD). This research provides preliminary evidence that methylation of DNMT3B may have functional significance with respect to colorectal adenomas, precursors to the vast majority of colorectal cancers.

  1. High DNA methyltransferase DNMT3B levels: a poor prognostic marker in acute myeloid leukemia.

    Directory of Open Access Journals (Sweden)

    Sandrine Hayette

    Full Text Available It has been recently shown that DNA methyl transferase overexpression is correlated with unfavourable prognosis in human malignancies while methylation deregulation remains a hallmark that defines acute myeloid leukemia (AML. The oncogenic transcription factor EVI1 is involved in methylation deregulation and its overexpression plays a major role for predicting an adverse outcome. Moreover, the identification of DNMT3A mutations in AML patients has recently been described as a poor prognostic indicator. In order to clarify relationship between these key actors in methylation mechanisms and their potential impact on patient outcomes, we analysed 195 de novo AML patients for the expression of DNMT3A, 3B (and its non-catalytic variant 3B(NC and their correlations with the outcome and the expression of other common prognostic genetic biomarkers (EVI1, NPM1, FLT3ITD/TKD and MLL in adult AML. The overexpression of DNMT3B/3B(NC is (i significantly correlated with a shorter overall survival, and (ii inversely significantly correlated with event-free survival and DNMT3A expression level. Moreover, multivariate analysis showed that a high expression level of DNMT3B/3B(NC is statistically a significant independent poor prognostic indicator. This study represents the first report showing that the overexpression of DNMT3B/3B(NC is an independent predictor of poor survival in AML. Its quantification should be implemented to the genetic profile used to stratify patients for therapeutical strategies and should be useful to identify patients who may benefit from therapy based on demethylating agents.

  2. The DNA cytosine deaminase APOBEC3B promotes tamoxifen resistance in ER-positive breast cancer

    Science.gov (United States)

    Law, Emily K.; Sieuwerts, Anieta M.; LaPara, Kelly; Leonard, Brandon; Starrett, Gabriel J.; Molan, Amy M.; Temiz, Nuri A.; Vogel, Rachel Isaksson; Meijer-van Gelder, Marion E.; Sweep, Fred C. G. J.; Span, Paul N.; Foekens, John A.; Martens, John W. M.; Yee, Douglas; Harris, Reuben S.

    2016-01-01

    Breast tumors often display extreme genetic heterogeneity characterized by hundreds of gross chromosomal aberrations and tens of thousands of somatic mutations. Tumor evolution is thought to be ongoing and driven by multiple mutagenic processes. A major outstanding question is whether primary tumors have preexisting mutations for therapy resistance or whether additional DNA damage and mutagenesis are necessary. Drug resistance is a key measure of tumor evolvability. If a resistance mutation preexists at the time of primary tumor presentation, then the intended therapy is likely to fail. However, if resistance does not preexist, then ongoing mutational processes still have the potential to undermine therapeutic efficacy. The antiviral enzyme APOBEC3B (apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3B) preferentially deaminates DNA C-to-U, which results in signature C-to-T and C-to-G mutations commonly observed in breast tumors. We use clinical data and xenograft experiments to ask whether APOBEC3B contributes to ongoing breast tumor evolution and resistance to the selective estrogen receptor modulator, tamoxifen. First, APOBEC3B levels in primary estrogen receptor–positive (ER+) breast tumors inversely correlate with the clinical benefit of tamoxifen in the treatment of metastatic ER+ disease. Second, APOBEC3B depletion in an ER+ breast cancer cell line results in prolonged tamoxifen responses in murine xenograft experiments. Third, APOBEC3B overexpression accelerates the development of tamoxifen resistance in murine xenograft experiments by a mechanism that requires the enzyme’s catalytic activity. These studies combine to indicate that APOBEC3B promotes drug resistance in breast cancer and that inhibiting APOBEC3B-dependent tumor evolvability may be an effective strategy to improve efficacies of targeted cancer therapies. PMID:27730215

  3. The Role of PDE3B Phosphorylation in the Inhibition of Lipolysis by Insulin

    OpenAIRE

    DiPilato, Lisa M.; Ahmad, Faiyaz; Harms, Matthew; Seale, Patrick; Manganiello, Vincent; Birnbaum, Morris J.

    2015-01-01

    Inhibition of adipocyte lipolysis by insulin is important for whole-body energy homeostasis; its disruption has been implicated as contributing to the development of insulin resistance and type 2 diabetes mellitus. The main target of the antilipolytic action of insulin is believed to be phosphodiesterase 3B (PDE3B), whose phosphorylation by Akt leads to accelerated degradation of the prolipolytic second messenger cyclic AMP (cAMP). To test this hypothesis genetically, brown adipocytes lacking...

  4. Volumes efetivos de anestésicos locais para o bloqueio do compartimento da fáscia ilíaca: estudo comparativo duplamente encoberto entre ropivacaína a 0,5% e bupivacaína a 0,5% Volúmenes efectivos de anestésicos locales para el bloqueo del compartimiento de la fascia ilíaca: estudio comparativo doblemente encubierto entre ropivacaína a 0,5% y bupivacaína a 0,5% Effective volume of local anesthetics for fascia iliac compartment block: a double-blind, comparative study between 0.5% ropivacaine and 0.5% bupivacaine

    Directory of Open Access Journals (Sweden)

    Pablo Escovedo Helayel

    2006-10-01

    utilizado volúmenes fijos de ropivacaína o de bupivacaína. Este estudio tuvo como objetivo calcular los volúmenes de ropivacaína a 0,5% y de bupivacaína a 0,5% efectivos en 50% (VE50, 95% (VE95 y 99% (VE99 de los casos para la realización de bloqueos del compartimento de la fascia ilíaca. MÉTODO: Cincuenta y un adultos con cirugías marcadas electivas de la cadera, diáfisis femoral y rodilla se sometieron al bloqueo del compartimento de la fascia ilíaca. Los pacientes fueron aleatoriamente distribuidos y recibieron ropivacaína a 0,5% (n = 25 o bupivacaína a 0,5% (n = 26. El éxito del bloqueo fue definido como bloqueo sensitivo completo de las regiones anterior, media y lateral del muslo. El volumen anestésico fue determinado por el método up-and-down de Massey y Dixon y los volúmenes efectivos fueron calculados por las formulas de Massey y Dixon (VE50 y por regresión de probits (VE50, VE95 y VE99. RESULTADOS: Los volúmenes anestésicos capaces de producir bloqueo nervioso efectivo en 50% de los casos, calculados por la formula de Massey y Dixon, fueron 28,79 mL (IC 95% : 26,31 -31,5 mL para ropivacaína y 29,56 mL (IC 95% : 25,22 - 34,64 mL para bupivacaína (p = 0,62. Los volúmenes efectivos de ropivacaína capaces de bloquear 50%, 95% y 99% de los casos se estimaron por la regresión de probits como 28,8 mL (27,2 - 30,4, 34,3 mL (32,5 - 37,3 y 36,6 mL (34,3 - 40,5, respectivamente. Los volúmenes correspondientes de bupivacaína fueron 29,5 mL (28,1 - 31,1, 36,1 mL (33,5 - 38,1, y 37,3 mL (35,1 - 41,3 (p > 0,05. CONCLUSIONES: Los volúmenes necesarios de ropivacaína a 0,5% y bupivacaína a 0,5% con adrenalina 1:200.000 para el bloqueo del compartimento de la fascia ilíaca son semejantes.BACKGROUND AND OBJECTIVES: Fascia iliac compartment block is widely used as one of the anesthetic techniques used for surgical interventions of the hip, thigh, and knee. The majority of the studies have used fixed volumes of ropivacaine or bupivacaine. The objective

  5. Evaluation of TRMM 3B43 Precipitation Data for Drought Monitoring in Jiangsu Province, China

    Directory of Open Access Journals (Sweden)

    Hui Tao

    2016-05-01

    Full Text Available Satellite-based precipitation monitoring at high spatial resolution is crucial for assessing the water and energy cycles at the global and regional scale. Based on the recently released 7th version of the Multi-satellite Precipitation Analysis (TMPA product of the Tropical Rainfall Measuring Mission (TRMM, and the monthly precipitation data (3B43 are evaluated using observed monthly precipitation from 65 meteorological stations in Jiangsu Province, China, for the period 1998–2014. Additionally, the standardized precipitation index (SPI, which is derived by a nonparametric approach, is employed to investigate the suitability of the TRMM 3B43 precipitation data for drought monitoring in Jiangsu Province. The temporal correlations between observations and the TRMM 3B43 precipitation data show, in general, reasonable agreement for different time scales. However, in summer, only 50% of the stations present correlation coefficients that are statistically significant at the 95% confidence interval. The overall best agreement of TRMM 3B43 precipitation data at seasonal scale tends to occur in autumn (SON. The comparative analysis of the calculated SPI time series suggests that the accuracy of TRMM3B43 decreases with increasing time scale. Stations with significant correlation coefficients also become less spatially homogeneous with increasing time scale. In summary, the findings demonstrate that TRMM 3B43 precipitation data can be used for reliable short-term drought monitoring in Jiangsu province, while temporal-spatial limitations exist for longer time scales.

  6. Arid3b is essential for second heart field cell deployment and heart patterning.

    Science.gov (United States)

    Uribe, Verónica; Badía-Careaga, Claudio; Casanova, Jesús C; Domínguez, Jorge N; de la Pompa, José Luis; Sanz-Ezquerro, Juan José

    2014-11-01

    Arid3b, a member of the conserved ARID family of transcription factors, is essential for mouse embryonic development but its precise roles are poorly understood. Here, we show that Arid3b is expressed in the myocardium of the tubular heart and in second heart field progenitors. Arid3b-deficient embryos show cardiac abnormalities, including a notable shortening of the poles, absence of myocardial differentiation and altered patterning of the atrioventricular canal, which also lacks epithelial-to-mesenchymal transition. Proliferation and death of progenitors as well as early patterning of the heart appear normal. However, DiI labelling of second heart field progenitors revealed a defect in the addition of cells to the heart. RNA microarray analysis uncovered a set of differentially expressed genes in Arid3b-deficient tissues, including Bhlhb2, a regulator of cardiomyocyte differentiation, and Lims2, a gene involved in cell migration. Arid3b is thus required for heart development by regulating the motility and differentiation of heart progenitors. These findings identify Arid3b as a candidate gene involved in the aetiology of human congenital malformations.

  7. An overview of age-related changes in the scalp distribution of P3b.

    Science.gov (United States)

    Friedman, D; Kazmerski, V; Fabiani, M

    1997-11-01

    In this overview of 7 studies, the scalp distribution of the P3b component (i.e. the P3 or P300) of the event-related potential elicited by target events in young and older adults was assessed. The target P3b data were recorded in either auditory oddball paradigms or in visual study tasks in which orienting activity was manipulated (as a within-subjects variable) in investigations of indirect memory. Some of the studies required choice reaction time responses, whereas others required responses only to the target stimuli. Motor response requirements had a profound effect on the P3b scalp distribution of older but not of younger subjects. The presence of a frontally oriented scalp focus in the topographies of the older adults in most of the tasks described here is consistent with older adults continuing to use prefrontal processes for stimuli that should have already been well encoded and/or categorized. However, although older subjects generally had different P3b scalp distributions than younger subjects, their scalp distributions were modulated similarly by task requirements. These data suggest that similar mechanisms modulate the scalp distribution of P3b in older compared to younger adults. However, in the older adult, these scalp distribution changes in response to task demands are superimposed on a frontally oriented scalp focus due to a putative frontal lobe contribution to target P3b topography.

  8. Mechanism of SEMA3B gene silencing and clinical significance in glioma.

    Science.gov (United States)

    Pang, C H; Du, W; Long, J; Song, L J

    2016-03-18

    The aim of the current study was to explore mechanisms of SEMA3B gene expression and its clinical significance in glioma, and provide a theoretical foundation for investigating individualized treatment in glioma. Paraffin-embedded tissues from 43 patients with a confirmed clinical diagnosis of glioma following neurosurgery at the First Affiliated Hospital of Zhengzhou University from December 2013 to April 2014 were selected randomly. An additional three normal brain tissues were obtained following encephalic decompression excision due to acute craniocerebral injury in the same period, which were used as the control group. Immunohistochemical staining for vascular endothelial growth factor was performed on the glioma tissues from the 43 patients. Genomic DNA was extracted for bisulfate conversion and sequencing. SEMA3B was fully expressed in the three normal brain tissues, and incompletely expressed in the 43 glioma tissues, with a lack of expression in 48.8% (21/43) of samples. Moreover, 58% of high-grade gliomas (grade III and IV) lacked SEMA3B expression, which was significantly more than those that lacked expression (20%) in low-grade gliomas (grade I and II), indicating that, as the clinical pathological grade increased, SEMA3B expression decreased. The occurrence and development of malignant tumors is a product of multiple genes and other factors. Here, we provide theoretical basis for glioma development and prognosis involving DNA-methylation driven silencing of SEMA3B, and thus, SEMA3B is a potential target for directed treatments against glioma.

  9. B-Myb Induces APOBEC3B Expression Leading to Somatic Mutation in Multiple Cancers

    Science.gov (United States)

    Chou, Wen-Cheng; Chen, Wei-Ting; Hsiung, Chia-Ni; Hu, Ling-Yueh; Yu, Jyh-Cherng; Hsu, Huan-Ming; Shen, Chen-Yang

    2017-01-01

    The key signature of cancer genomes is the accumulation of DNA mutations, the most abundant of which is the cytosine-to-thymine (C-to-T) transition that results from cytosine deamination. Analysis of The Cancer Genome Atlas (TCGA) database has demonstrated that this transition is caused mainly by upregulation of the cytosine deaminase APOBEC3B (A3B), but the mechanism has not been completely characterized. We found that B-Myb (encoded by MYBL2) binds the A3B promoter, causing transactivation, and this is responsible for the C-to-T transitions and DNA hypermutation in breast cancer cells. Analysis of TCGA database yielded similar results, supporting that MYBL2 and A3B are upregulated and putatively promote C-to-T transitions in multiple cancer types. Moreover, blockade of EGF receptor with afatinib attenuated B-Myb–A3B signaling, suggesting a clinically relevant means of suppressing mutagenesis. Our results suggest that B-Myb–A3B contributes to DNA damage and could be targeted by inhibiting EGF receptor. PMID:28276478

  10. M3B: A coarse grain model for the simulation of oligosaccharides and their water mixtures.

    Science.gov (United States)

    Goddard, William A.; Cagin, Tahir; Molinero, Valeria

    2003-03-01

    Water and sugar dynamics in concentrated carbohydrate solutions is of utmost importance in food and pharmaceutical technology. Water diffusion in concentrated sugar mixtures can be slowed down many orders of magnitude with respect to bulk water [1], making extremely expensive the simulation of these systems with atomistic detail for the required time-scales. We present a coarse grain model (M3B) for malto-oligosaccharides and their water mixtures. M3B speeds up molecular dynamics simulations about 500-1000 times with respect to the atomistic model while retaining enough detail to be mapped back to the atomistic structures with low uncertainty in the positions. The former characteristic allows the study of water and carbohydrate dynamics in supercooled and polydisperse mixtures with characteristic time scales above the nanosecond. The latter makes M3B well suited for combined atomistic-mesoscale simulations. We present the parameterization of M3B force field for water and a family of technologically relevant glucose oligosaccharides, the alpha-(1->4) glucans. The coarse grain force field is completely parameterized from atomistic simulations to reproduce the density, cohesive energy and structural parameters of amorphous sugars. We will show that M3B is capable to describe the helical character of the higher oligosaccharides, and that the water structure in low moisture mixtures shows the same features obtained with the atomistic and M3B models. [1] R Parker, SG Ring: Carbohydr. Res. 273 (1995) 147-55.

  11. Dnmt3a and Dnmt3b have overlapping and distinct functions in hematopoietic stem cells.

    Science.gov (United States)

    Challen, Grant A; Sun, Deqiang; Mayle, Allison; Jeong, Mira; Luo, Min; Rodriguez, Benjamin; Mallaney, Cates; Celik, Hamza; Yang, Liubin; Xia, Zheng; Cullen, Sean; Berg, Jonathan; Zheng, Yayun; Darlington, Gretchen J; Li, Wei; Goodell, Margaret A

    2014-09-04

    Epigenetic regulation of hematopoietic stem cells (HSCs) ensures lifelong production of blood and bone marrow. Recently, we reported that loss of de novo DNA methyltransferase Dnmt3a results in HSC expansion and impaired differentiation. Here, we report conditional inactivation of Dnmt3b in HSCs either alone or combined with Dnmt3a deletion. Combined loss of Dnmt3a and Dnmt3b was synergistic, resulting in enhanced HSC self-renewal and a more severe block in differentiation than in Dnmt3a-null cells, whereas loss of Dnmt3b resulted in a mild phenotype. Although the predominant Dnmt3b isoform in adult HSCs is catalytically inactive, its residual activity in Dnmt3a-null HSCs can drive some differentiation and generates paradoxical hypermethylation of CpG islands. Dnmt3a/Dnmt3b-null HSCs displayed activated β-catenin signaling, partly accounting for the differentiation block. These data demonstrate distinct roles for Dnmt3b in HSC differentiation and provide insights into complementary de novo methylation patterns governing regulation of HSC fate decisions.

  12. Loss of Dnmt3b accelerates MLL-AF9 leukemia progression.

    Science.gov (United States)

    Zheng, Y; Zhang, H; Wang, Y; Li, X; Lu, P; Dong, F; Pang, Y; Ma, S; Cheng, H; Hao, S; Tang, F; Yuan, W; Zhang, X; Cheng, T

    2016-12-01

    Acute myeloid leukemia (AML) is a heterogeneous hematopoietic disorder with a poor prognosis. Abnormal DNA methylation is involved in the initiation and progression of AML. The de novo methyltransferases Dnmt3a and Dnmt3b are responsible for the generation of genomic methylation patterns. While DNMT3A is frequently mutated in hematological malignancies, DNMT3B is rarely mutated. Although it has been previously reported that Dnmt3b functions as a tumor suppressor in a mouse model of Myc-induced lymphomagenesis, its function in AML is yet to be determined. In this study, we demonstrated that deletion of Dnmt3b accelerated the progression of MLL-AF9 leukemia by increasing stemness and enhancing cell cycle progression. Gene profiling analysis revealed upregulation of the oncogenic gene set and downregulation of the cell differentiation gene set. Furthermore, loss of Dnmt3b was able to synergize with Dnmt3a deficiency in leukemia development. Taken together, these results demonstrate that Dnmt3b plays a tumor suppressive role in MLL-AF9 AML progression, thereby providing new insights into the roles of DNA methylation in leukemia development.

  13. Shostakovich: The Orchestral Songs Vol. 2 / Michael Tanner

    Index Scriptorium Estoniae

    Tanner, Michael

    1996-01-01

    Uuest heliplaadist "Shostakovich: The Orchestral Songs Vol. 2: Six Romances on texts by Japanese poets, Op. 21. Six Poems on Marina Tsvetayeva, Op. 143. Suite on Verses of Michelangelo, Op. 145. Gothenburg Symphony Orchestra, Neeme Järvi". DG 447 085-2GH (71 minutes:DDD)

  14. Schostakowitsch. Orchesterlieder (Vol. 2), Neeme Järvi / Werner Pfister

    Index Scriptorium Estoniae

    Pfister, Werner

    1996-01-01

    Uuest heliplaadist "Schostakowitsch. Orchesterlieder (Vol. 2): Sechs Romanzen op. 21, Sechs Gedichte op. 143a, Suite auf Verse von Michelangelo Buonarroti op. 145a. Göteborger Sinfoniker, Neeme Järvi". DG CD 447 085-2 (WD: 71'06") DDD

  15. East and Central African Journal of Pharmaceutical Sciences Vol. 7 ...

    African Journals Online (AJOL)

    East and Central African Journal of Pharmaceutical Sciences. Vol. 7(3) 52-55. Adherence ... any for the rest of hislher life is one of the biggest challenges. While much .... Secondly, 2- and 3-times-daily regimens required for HIV are difficult and ...

  16. Tropical Journal of Pharmaceutical Research - Vol 16, No 2 (2017)

    African Journals Online (AJOL)

    Tropical Journal of Pharmaceutical Research - Vol 16, No 2 (2017) ... Bone regeneration potential of sub-microfibrous membranes with osteogenic ... Baicalein and U0126 suppress bladder cancer proliferation via MAPK ... Analysis of the effect of Qizhuyigan on liver function in a mouse model of immunological liver injury ...

  17. Vol 39 2011 Art 5.pub - African Journals Online

    African Journals Online (AJOL)

    p2613611

    ISSN 0378-5254 Journal of Family Ecology and Consumer Sciences, Vol 39, 2011 ... The pertinence of status factors in consumers' consideration of major ... ties in terms of consumers' expectations and appli- ... such characteristics serve as pertinent heuristics in ... value and the quality of appliances from their external.

  18. Shostakovich: The Orchestral Songs Vol. 2 / Michael Tanner

    Index Scriptorium Estoniae

    Tanner, Michael

    1996-01-01

    Uuest heliplaadist "Shostakovich: The Orchestral Songs Vol. 2: Six Romances on texts by Japanese poets, Op. 21. Six Poems on Marina Tsvetayeva, Op. 143. Suite on Verses of Michelangelo, Op. 145. Gothenburg Symphony Orchestra, Neeme Järvi". DG 447 085-2GH (71 minutes:DDD)

  19. Schostakowitsch. Orchesterlieder (Vol. 2), Neeme Järvi / Werner Pfister

    Index Scriptorium Estoniae

    Pfister, Werner

    1996-01-01

    Uuest heliplaadist "Schostakowitsch. Orchesterlieder (Vol. 2): Sechs Romanzen op. 21, Sechs Gedichte op. 143a, Suite auf Verse von Michelangelo Buonarroti op. 145a. Göteborger Sinfoniker, Neeme Järvi". DG CD 447 085-2 (WD: 71'06") DDD

  20. New Egyptian Journal of Microbiology - Vol 17 (2007)

    African Journals Online (AJOL)

    New Egyptian Journal of Microbiology - Vol 17 (2007) ... Detection Of Human Herpes Virus Type-8 Dna In Kaposi\\'s Sarcoma And Other Bullous Skin Diseases · EMAIL ... Of Conventional And Molecular Diagnosis Of Superficial Fungal Infections .... Interference Between Lactobacilli And Group A Streptococcus pyogenes: An ...

  1. African Journal of Infectious Diseases - Vol 10, No 2 (2016)

    African Journals Online (AJOL)

    African Journal of Infectious Diseases - Vol 10, No 2 (2016) ... The strengths, weaknesses, opportunities, and threats (SWOTs) analyses of the Ebola virus ... ducks as potential reservoir of avian influenza virus in post HPAI H5N1 outbreak area, ...

  2. Creative Artist: A Journal of Theatre and Media Studies - Vol 10, No ...

    African Journals Online (AJOL)

    Creative Artist: A Journal of Theatre and Media Studies - Vol 10, No 1 (2016). Journal Home > Archives > Vol 10, No 1 (2016) ... Community theatre and development practices in Nyanza Region, Kenya · EMAIL FREE FULL TEXT EMAIL FREE ...

  3. Ternary borides Nb7Fe3B8 and Ta7Fe3B8 with Kagome-type iron framework.

    Science.gov (United States)

    Zheng, Qiang; Gumeniuk, Roman; Borrmann, Horst; Schnelle, Walter; Tsirlin, Alexander A; Rosner, Helge; Burkhardt, Ulrich; Reissner, Michael; Grin, Yuri; Leithe-Jasper, Andreas

    2016-06-21

    Two new ternary borides TM7Fe3B8 (TM = Nb, Ta) were synthesized by high-temperature thermal treatment of samples obtained by arc-melting. This new type of structure with space group P6/mmm, comprises TM slabs containing isolated planar hexagonal [B6] rings and iron centered TM columns in a Kagome type of arrangement. Chemical bonding analysis in Nb7Fe3B8 by means of the electron localizability approach reveals two-center interactions forming the Kagome net of Fe and embedded B, while weaker multicenter bonding present between this net and Nb atoms. Magnetic susceptibility measurements reveal antiferromagnetic order below TN = 240 K for Nb7Fe3B8 and TN = 265 K for Ta7Fe3B8. Small remnant magnetization below 0.01μB per f.u. is observed in the antiferromagnetic state. The bulk nature of the magnetic transistions was confirmed by the hyperfine splitting of the Mössbauer spectra, the sizable anomalies in the specific heat capacity, and the kinks in the resistivity curves. The high-field paramagnetic susceptibilities fitted by the Curie-Weiss law show effective paramagnetic moments μeff≈ 3.1μB/Fe in both compounds. The temperature dependence of the electrical resistivity also reveals metallic character of both compounds. Density functional calculations corroborate the metallic behaviour of both compounds and demonstrate the formation of a sizable local magnetic moment on the Fe-sites. They indicate the presence of both antiferro- and ferrromagnetic interactions.

  4. Al SahawaThe Awakening: Volume 3-B: Al Anbar Province Area of Operations Denver, HadithahHit Corridor

    Science.gov (United States)

    2016-05-01

    normal meat and potatoes CAX, the only thing we cut off was the FINEX [final exercise] at the end, but we had all the combined arms things first. Even...single street corner in Hadithah. We pushed back on that guidance. Not knowing what we didn’t know, and even though we had done the map and cultural ...a very intellectually flexible, culturally adept Marine. At the end of OIF I, when we were in Baghdad and we had gone firm and were starting to work

  5. Aberrant expression and localization of deoxyribonucleic acid methyltransferase 3B in endometriotic stromal cells.

    Science.gov (United States)

    Dyson, Matthew T; Kakinuma, Toshiyuki; Pavone, Mary Ellen; Monsivais, Diana; Navarro, Antonia; Malpani, Saurabh S; Ono, Masanori; Bulun, Serdar E

    2015-10-01

    To define the expression and function of DNA methyltransferases (DNMTs) in response to decidualizing stimuli in endometriotic cells compared with healthy endometrial stroma. Basic science. University research center. Premenopausal women with or without endometriosis. Primary cultures of stromal cells from healthy endometrium (E-IUM) or endometriomas (E-OSIS) were subjected to in vitro decidualization (IVD) using 1 μM medroxyprogesterone acetate, 35 nM 17β-estradiol, and 0.05 mM 8-Br-cAMP. Expression of DNMT1, DNMT3A, and DNMT3B in E-IUM and E-OSIS were assessed by quantitative real-time polymerase chain reaction and immunoblotting. Recruitment of DNMT3B to the promoters of steroidogenic factor 1 (SF-1) and estrogen receptor α (ESR1) was examined by chromatin immunoprecipitation. IVD treatment reduced DNMT3B messenger RNA (74%) and protein levels (81%) only in E-IUM; DNMT1 and DNMT3A were unchanged in both cell types. Significantly more DNMT3B bound to the SF-1 promoter in E-IUM compared with E-OSIS, and IVD treatment reduced binding in E-IUM to levels similar to those in E-OSIS. Enrichment of DNMT3B across 3 ESR1 promoters was reduced in E-IUM after IVD, although the more-distal promoter showed increased DNMT3B enrichment in E-OSIS after IVD. The inability to downregulate DNMT3B expression in E-OSIS may contribute to an aberrant epigenetic fingerprint that misdirects gene expression in endometriosis and contributes to its altered response to steroid hormones. Copyright © 2015 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

  6. The aberrant expression and localization of DNA methyltransferase 3B in endometriotic stromal cells

    Science.gov (United States)

    Dyson, Matthew T.; Kakinuma, Toshiyuki; Pavone, Mary Ellen; Monsivais, Diana; Navarro, Antonia; Malpani, Saurabh S.; Ono, Masanori; Bulun, Serdar E.

    2015-01-01

    Objective To define the expression and function of DNA methyltransferases (DNMTs) in response to decidualizing stimuli in endometriotic cells compared with healthy endometrial stroma. Design Basic science. Setting University research center. Patients Premenopausal women with or without endometriosis. Interventions Primary cultures of stromal cells from healthy endometrium (E-IUM) or endometriomas (E-OSIS) were subjected to in vitro decidualization (IVD) using 1 µM medroxyprogesterone acetate, 35 nM 17β-estradiol, and 0.05 mM 8-Br-cAMP. Main Outcome Measure(s) DNMT1, DNMT3A, and DNMT3B expression in E-IUM and E-OSIS were assessed by qRT-PCR and immunoblotting. DNMT3B recruitment to the promoters of steroidogenic factor 1 (SF-1) and estrogen receptor α (ESR1) was examined by chromatin immunoprecipitation Results IVD treatment reduced DNMT3B mRNA (74%) and protein levels (81%) only in E-IUM. DNMT1 and DNMT3A were unchanged in both cell types. Significantly more DNMT3B bound to the SF-1 promoter in E-IUM compared with E-OSIS, and IVD treatment reduced binding in E-IUM to levels similar to those in E-OSIS. DNMT3B enrichment across three ESR1 promoters was reduced in E-IUM after IVD, although the more distal promoter showed increased DNMT3B enrichment in E-OSIS after IVD. Conclusions The inability to downregulate DNMT3B expression in E-OSIS may contribute to an aberrant epigenetic fingerprint that misdirects gene expression in endometriosis and contributes to its altered response to steroid hormones. PMID:26239024

  7. SF3B1 mutations constitute a novel therapeutic target in breast cancer

    Science.gov (United States)

    Maguire, Sarah L; Leonidou, Andri; Wai, Patty; Marchiò, Caterina; Ng, Charlotte KY; Sapino, Anna; Salomon, Anne-Vincent; Reis-Filho, Jorge S; Weigelt, Britta; Natrajan, Rachael C

    2015-01-01

    Mutations in genes encoding proteins involved in RNA splicing have been found to occur at relatively high frequencies in several tumour types including myelodysplastic syndromes, chronic lymphocytic leukaemia, uveal melanoma, and pancreatic cancer, and at lower frequencies in breast cancer. To investigate whether dysfunction in RNA splicing is implicated in the pathogenesis of breast cancer, we performed a re-analysis of published exome and whole genome sequencing data. This analysis revealed that mutations in spliceosomal component genes occurred in 5.6% of unselected breast cancers, including hotspot mutations in the SF3B1 gene, which were found in 1.8% of unselected breast cancers. SF3B1 mutations were significantly associated with ER-positive disease, AKT1 mutations, and distinct copy number alterations. Additional profiling of hotspot mutations in a panel of special histological subtypes of breast cancer showed that 16% and 6% of papillary and mucinous carcinomas of the breast harboured the SF3B1 K700E mutation. RNA sequencing identified differentially spliced events expressed in tumours with SF3B1 mutations including the protein coding genes TMEM14C, RPL31, DYNL11, UQCC, and ABCC5, and the long non-coding RNA CRNDE. Moreover, SF3B1 mutant cell lines were found to be sensitive to the SF3b complex inhibitor spliceostatin A and treatment resulted in perturbation of the splicing signature. Albeit rare, SF3B1 mutations result in alternative splicing events, and may constitute drivers and a novel therapeutic target in a subset of breast cancers. © 2014 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland. PMID:25424858

  8. Kaempferol Modulates DNA Methylation and Downregulates DNMT3B in Bladder Cancer

    Directory of Open Access Journals (Sweden)

    Wei Qiu

    2017-03-01

    Full Text Available Background: Genomic DNA methylation plays an important role in both the occurrence and development of bladder cancer. Kaempferol (Kae, a natural flavonoid that is present in many fruits and vegetables, exhibits potent anti-cancer effects in bladder cancer. Similar to other flavonoids, Kae possesses a flavan nucleus in its structure. This structure was reported to inhibit DNA methylation by suppressing DNA methyltransferases (DNMTs. However, whether Kae can inhibit DNA methylation remains unclear. Methods: Nude mice bearing bladder cancer were treated with Kae for 31 days. The genomic DNA was extracted from xenografts and the methylation changes was determined using an Illumina Infinium HumanMethylation 450 BeadChip Array. The ubiquitination was detected using immuno-precipitation assay. Results: Our data indicated that Kae modulated DNA methylation in bladder cancer, inducing 103 differential DNA methylation positions (dDMPs associated with genes (50 hyper-methylated and 53 hypo-methylated. DNA methylation is mostly relied on the levels of DNMTs. We observed that Kae specifically inhibited the protein levels of DNMT3B without altering the expression of DNMT1 or DNMT3A. However, Kae did not downregulate the transcription of DNMT3B. Interestingly, we observed that Kae induced a premature degradation of DNMT3B by inhibiting protein synthesis with cycloheximide (CHX. By blocking proteasome with MG132, we observed that Kae induced an increased ubiquitination of DNMT3B. These results suggested that Kae could induce the degradation of DNMT3B through ubiquitin-proteasome pathway. Conclusion: Our data indicated that Kae is a novel DNMT3B inhibitor, which may promote the degradation of DNMT3B in bladder cancer.

  9. Genetic and epigenetic variation in the DNMT3B and MTHFR genes and colorectal adenoma risk.

    Science.gov (United States)

    Ho, Vikki; Ashbury, Janet E; Taylor, Sherryl; Vanner, Stephen; King, Will D

    2016-05-01

    Polymorphisms in DNMT3B and MTHFR have been implicated in cancer etiology; however, it is increasingly clear that gene-specific DNA methylation also affects gene expression. A cross-sectional study (N = 272) investigated the main and joint effects of polymorphisms and DNA methylation in DNMT3B and MTHFR on colorectal adenoma risk. Polymorphisms examined included DNMT3B c.-6-1045G > T, and MTHFR c.665C > T and c.1286A > C. DNA methylation of 66 and 28 CpG sites in DNMT3B and MTHFR, respectively, was quantified in blood leukocytes using Sequenom EpiTYPER®. DNA methylation was conceptualized using two approaches: (1) average methylation and (2) unsupervised principal component analysis to identify variables that represented methylation around the transcription start site and the gene coding area of both genes. Logistic regression was used to estimate odds ratios (ORs) and 95% confidence intervals (CIs) associated with the main and joint effects of polymorphisms and DNA methylation. DNA methyltransferase 3B (DNMT3B) TT versus GG/GT genotypes was associated with increased colorectal adenoma risk (OR = 2.12; 95% CI: 1.03-4.34). In addition, increasing DNA methylation in the gene-coding area of DNMT3B was associated with higher risk of colorectal adenomas (OR = 1.34; 95% CI: 1.01-1.79 per SD). In joint effect analyses, synergistic effects were observed among those with both the DNMT3B TT genotype and higher DNMT3B methylation levels compared to those with GT/GG genotypes and lower methylation levels (OR = 4.19; 95% CI: 1.45-12.13 for average methylation; OR = 4.26; 95%CI: 1.31-13.87 for methylation in the transcription start site). This research provides novel evidence that genetic and epigenetic variations contribute to colorectal adenoma risk, precursor to the majority of colorectal cancer (CRC).

  10. Synthesis and Reactions of Furo[2,3-b]pyrroles

    Directory of Open Access Journals (Sweden)

    A. Lycka

    1997-04-01

    Full Text Available Methyl 6H-furo[2,3-b]pyrrole-5-carboxylate (2a was prepared by thermolysis of the corresponding methyl 2-azido-3-(3-furylpropenoate (1. 6-Methyl (2b and 6-benzyl (2c derivatives were obtained using phase-transfer catalysis conditions (PTC. The formylation of 2a-2c gave 2-formylated compounds (3a-3c. Compounds 4b, 4c were prepared by reactions of corresponding esters 2b, 2c with hydrazine in refluxing ethanol. By reaction of 3a-3c with hydroxylammonium chloride in acetic anhydride in the presence of pyridine, methyl 2-cyano-6-R1-furo[2,3-b]pyrrole-5-carboxylates (5a-5c were obtained. The reaction of these compounds with sodium azide and ammonium chloride in dimethylformamide led to methyl 2-(5'-tetrazolyl-6-R1-furo[2,3-b]pyrrole-5-carboxylates (6a-6c. A series of 5-methoxycarbonyl-6-R1-furo[2,3-b]pyrrole-2-carbaldehyde N,N-dimethylhydrazones (7a-7c was prepared from methyl 2-formyl-6-R1-furo[2,3-b]pyrrole-5-carboxylates (3a-3c and unsym-dimethylhydrazine. The correlation of the 13C and 15N chemical shifts with the data of the calculated (AM1 net atomic charges is discussed.

  11. Upk3b is dispensable for development and integrity of urothelium and mesothelium.

    Directory of Open Access Journals (Sweden)

    Carsten Rudat

    Full Text Available The mesothelium, the lining of the coelomic cavities, and the urothelium, the inner lining of the urinary drainage system, are highly specialized epithelia that protect the underlying tissues from mechanical stress and seal them from the overlying fluid space. The development of these epithelia from simple precursors and the molecular characteristics of the mature tissues are poorly analyzed. Here, we show that uroplakin 3B (Upk3b, which encodes an integral membrane protein of the tetraspanin superfamily, is specifically expressed both in development as well as under homeostatic conditions in adult mice in the mesothelia of the body cavities, i.e., the epicardium and pericardium, the pleura and the peritoneum, and in the urothelium of the urinary tract. To analyze Upk3b function, we generated a creERT2 knock-in allele by homologous recombination in embryonic stem cells. We show that Upk3bcreERT2 represents a null allele despite the lack of creERT2 expression from the mutated locus. Morphological, histological and molecular analyses of Upk3b-deficient mice did not detect changes in differentiation or integrity of the urothelium and the mesothelia that cover internal organs. Upk3b is coexpressed with the closely related Upk3a gene in the urothelium but not in the mesothelium, leaving the possibility of a functional redundancy between the two genes in the urothelium only.

  12. The Study of DNA Methyltransferase-3B Promoter Variant Genotype among Iranian Sporadic Breast Cancer Patients

    Directory of Open Access Journals (Sweden)

    Ebrahim Eftekhar

    2014-05-01

    Full Text Available Background: DNA methyltransferase-3B (DNMT3B is an important enzyme responsible for maintaining the DNA methylation pattern in eukaryotic cells. In this study we have investigated the correlation between the 46359C→T polymorphism in the DNMT3B gene and the risk of breast cancer incidence among sporadic breast cancer patients in Fars Province, Southern Iran. Methods: In this case-control study, 100 breast cancer patients and 138 healthy control subjects were genotyped for the DNMT3B gene by the polymerase chain reaction-restriction fragment length polymorphism method. Results: The genotype frequency in the case (CC 27%, CT 47%, TT 26% group significantly (P=0.008 differed from the control (CC 19.56%, CT 67.3%, TT 13% group. We observed a decreased association between the CT genotype and lymph node involvement in breast cancer patients. Our results have shown that in comparison to the homozygous CC genotype carriers the DNMT3B-CT genotype has a significantly lower risk for breast cancer (OR=0.515, 95% CI=0.267-0.994, P=0.048. Conclusion: Our case-control study showed that the CT genotype was significantly associated with decreased breast cancer risk. Consistent with these results, a significant decrease of CT genotype among lymph node positive breast cancer patients was observed. However, a larger study population with more clinical data is needed to confirm these results.

  13. DNMT3B 579 G>T promoter polymorphism and risk of esophagus carcinoma in Chinese

    Institute of Scientific and Technical Information of China (English)

    Hong Fan; Dong-Sheng Liu; Shu-Hong Zhang; Jia-Bo Hu; Feng Zhang; Zhu-Jiang Zhao

    2008-01-01

    AIM: To investigate the relationship between 579 G>T polymorphisms in the DNMT3B gene, which is involved in de novo methylation and associated with the risk of esophagus cancer (EC) in Chinese.METHODS: DNMT3B 579 G>T genotypes were determined by PCR-RFLP in 194 EC patients and 210 healthy controls matched for age and sex, who did not receive radiotherapy or chemotherapy for newly diagnosed and histopathologically confirmed EC.RESULTS: In control subjects, the frequency of T/T and G/T genotypes, and T and G alleles was 81.4%, 18.1%,90.05% and 9.55%, respectively. The distribution of genotypes and allelotypes in the EC patients was not significantly different from that in the controls.When stratified by sex and age, there was still no significant association between the risks of EC and GT and GG genotypes. This study also showed a distinct difference in the distribution of DNMT3B and single nucleotide polymorphism (SNP) between Chinese and Koreans.CONCLUSION: DNMT3B 579 G>T polymorphism may not be a stratification marker to predict the susceptibility to EC, at least in Chinese. DNMT3B promoter SNP is diverse in ethnic populations.

  14. Global identification of genes targeted by DNMT3b for epigenetic silencing in lung cancer.

    Science.gov (United States)

    Teneng, I; Tellez, C S; Picchi, M A; Klinge, D M; Yingling, C M; Snider, A M; Liu, Y; Belinsky, S A

    2015-01-29

    The maintenance cytosine DNA methyltransferase DNMT1 and de novo methyltransferase DNMT3b cooperate to establish aberrant DNA methylation and chromatin complexes to repress gene transcription during cancer development. The expression of DNMT3b was constitutively increased 5-20-fold in hTERT/CDK4-immortalized human bronchial epithelial cells (HBECs) before treatment with low doses of tobacco carcinogens. Overexpression of DNMT3b increased and accelerated carcinogen-induced transformation. Genome-wide profiling of transformed HBECs identified 143 DNMT3b-target genes, many of which were transcriptionally regulated by the polycomb repressive complex 2 (PRC2) complex and silenced through aberrant methylation in non-small-cell lung cancer cell lines. Two genes studied in detail, MAL and OLIG2, were silenced during transformation, initially through enrichment for H3K27me3 and H3K9me2, commonly methylated in lung cancer, and exert tumor suppressor effects in vivo through modulating cancer-related pathways. Re-expression of MAL and OLIG2 to physiological levels dramatically reduced the growth of lung tumor xenografts. Our results identify a key role for DNMT3b in the earliest stages of initiation and provide a comprehensive catalog of genes targeted for silencing by this methyltransferase in non-small-cell lung cancer.

  15. DNMT3B promoter polymorphism and risk of immune thrombocytopenic purpura in pediatric Egyptians.

    Science.gov (United States)

    Shaheen, Iman A; Abukhalil, Reham E; Ali, Dina K; Afifi, Rasha A

    2012-10-01

    Idiopathic (immune) thrombocytopenic purpura (ITP) is a heterogeneous clinical disorder characterized by immune-mediated platelet destruction. Epigenetic changes in gene expression, including DNA methylation and histone modifications, might contribute to autoimmunity. Polymorphisms of the DNA methyltransferase 3B (DNMT3B) gene may influence DNMT3B activity on DNA methylation and increase the susceptibility to several diseases. The current study investigated the association between a single nucleotide polymorphism (SNP) in the promoter of DNMT3B gene and the risk for ITP in pediatric Egyptians. DNMT3B SNP was genotyped by PCR-restriction fragment length polymorphism in 71 pediatric ITP patients and 82 healthy controls matched for age and sex. The C/C wild genotype was not detected in ITP patients or in the controls. The frequencies of the T/T and C/T genotypes were 93.9 and 6.1% in the controls and 91.5 and 6.1% in ITP patients, respectively. There was no significant difference in either genotypes or allelic distribution between ITP patients and the controls. In conclusion, this polymorphism was almost equally distributed between ITP patients and the controls. These results demonstrated that this SNP may not be used as a stratification marker to predict the susceptibility to childhood ITP in Egypt.

  16. An association between overexpression of DNA methyltransferase 3B4 and clear cell renal cell carcinoma.

    Science.gov (United States)

    Liu, You; Sun, Liantao; Fong, Peter; Yang, Jie; Zhang, Zhuxia; Yin, Shuihui; Jiang, Shuyuan; Liu, Xiaolei; Ju, Hongge; Huang, Lihua; Bai, Jing; Gong, Kerui; Yan, Shaochun; Zhang, Chunyang; Shao, Guo

    2017-02-01

    It is well known that abnormal DNA methylations occur frequently in kidney cancer. However, it remains unclear exactly which types of DNA methyltransferases (DNMT) contribute to the pathologies of kidney cancers. In order to determine the functions of DNA methyltransferase in kidney tumorigenesis on the molecular level, we examined the mRNA expression levels of DNMT1, DNMT3A, DNMT3B, and DNMT3B variants in renal cell carcinoma tissue. Both mRNA and protein levels of DNMT3B4, a splice variant of DNMT3B, were increased in renal cell carcinoma tissue compared with adjacent control tissues. Additionally, Alu elements and long interspersed nuclear elements (LINE-1) were hypomethylated in renal cell carcinoma tissue. Meanwhile, methylation of the promoter for RASSF1A, a tumor suppressor gene, was moderately increased in renal cell carcinoma tissue, while RASSF1A expression was decreased. Thus, our data suggest that the overexpression of DNMT3B4 may play an important role in human kidney tumorigenesis through chromosomal instability and methylation of RASSF1A.

  17. Aberrant CBFA2T3B gene promoter methylation in breast tumors

    Directory of Open Access Journals (Sweden)

    Bais Anthony J

    2004-08-01

    Full Text Available Abstract Background The CBFA2T3 locus located on the human chromosome region 16q24.3 is frequently deleted in breast tumors. CBFA2T3 gene expression levels are aberrant in breast tumor cell lines and the CBFA2T3B isoform is a potential tumor suppressor gene. In the absence of identified mutations to further support a role for this gene in tumorigenesis, we explored whether the CBFA2T3B promoter region is aberrantly methylated and whether this correlates with expression. Results Aberrant hypo and hypermethylation of the CBFA2T3B promoter was detected in breast tumor cell lines and primary breast tumor samples relative to methylation index interquartile ranges in normal breast counterpart and normal whole blood samples. A statistically significant inverse correlation between aberrant CBFA2T3B promoter methylation and gene expression was established. Conclusion CBFA2T3B is a potential breast tumor suppressor gene affected by aberrant promoter methylation and gene expression. The methylation levels were quantitated using a second-round real-time methylation-specific PCR assay. The detection of both hypo and hypermethylation is a technicality regarding the methylation methodology.

  18. FCGR3B gene frequencies among ethnic Thai blood donors from a regional hospital in Eastern Thailand.

    Science.gov (United States)

    Kongmaroeng, C; Kumkaen, K

    2015-02-01

    The FCGR3B gene encodes three human neutrophil antigens which consist of HNA-1a, HNA-1b, and HNA-1c. These antigens are encoded by three alleles in the FCGR3B locus: FCGR3B*01, FCGR3B*02, and FCGR3B*03 alleles, respectively. The frequencies of FCGR3B alleles have been reported in different ethnic populations. This study compared the FCGR3B gene frequencies among 230 unrelated healthy Eastern Thai blood donors in Rayong hospital with the previously published studies. The polymerase chain reaction-sequence-specific primers method was performed to determine FCGR3B genotypes. The results showed that the allele frequencies of FCGR3B*01, FCGR3B*02, and FCGR3B*03 were 0.722, 0.274, and 0.009, respectively. The FCGR3B*01 and FCGR3B*02 frequencies found in the Eastern Thais were similar to the previous reports investigating in Northern Thais, Chinese Han, Taiwanese, and Japanese populations. Interestingly, our data showed statistically significant difference (P American, German, and Italian populations. In addition, one FCGR3Bnull , which represents a gene deletion, was also found in this study. This information is important not only for the assessment of neutrophil antibody-mediated clinical conditions and for disease association studies but also for anthropological studies.

  19. United States Air Force Summer Research Program -- 1993. Volume 16. Arnold Engineering Development Center. Frank J. Seiler Research Laboratory. Wilford Hall Medical Center

    Science.gov (United States)

    1993-12-01

    Laboratory: AEDC/ North Jackson St. Vol-Page No: 16- 1 Tullahoma Nigh School Tullahoma, TN 37388-0000 Bowlby , Andrea Laboratory: PL/GP Mudge Way Vol-Page No...January 1982. New York: Scientific American, Inc. 2. Charles Hirsch. Numerical Computation of Internal and External Flows, Volumes 1 & 2. Chichester: John

  20. Extinction coefficient of H2CC(3B2) at 137 nm

    Science.gov (United States)

    Fahr, A.; Laufer, A. H.

    1985-01-01

    In spite of the conduction of numerous studies regarding the vinylidene free radical, its role and importance as a reactive intermediate is not well characterized. Laufer (1980, 1983) has reported the absorption spectrum of metastable H2CC(3B2), the lowest excited state, in the vacuum ultraviolet and has measured several aspects of its quenching properties. The present study provides a measurement of the extinction coefficient of H2CC(3B2). Knowledge of the vinylidene concentration is required to convert readily available absorption data into an extinction coefficient or cross section. In the current work, the H2CC(3B2) concentration was determined in an investigation of the photodissociation of vinyl chloride.

  1. Study on the Adsorption Kinetics of Acid Red 3B on Expanded Graphite

    Directory of Open Access Journals (Sweden)

    Xiu-yan Pang

    2008-01-01

    Full Text Available Expanded graphite (EG is a kind of important adsorbent for organic compound such as oil and dyes. We have investigated the adsorption kinetics characteristics of this adsorbent for dye. EG was prepared with 50 mesh crude graphite through chemical oxidation intercalation of potassium permanganate and vitriol, and dye of acid red 3B was used as model sorbate. We have studied the adsorption kinetic models and rate-limiting step of the process. Adsorption rate and activation energy of the adsorption process were calculated. Kinetic studies show that the kinetic data are well described by the pseudo second-order kinetic model. The equilibrium adsorbance increases with the increase of the initial acid red 3B concentration. Initial adsorption rate increases with the increase of the initial dye concentration and temperature. Adsorption process of acid red 3B on EG has small activation energy. Internal diffusion appears to be the rate-limiting step for the adsorption process.

  2. Photometric investigation of hot exoplanets: TrES-3b and Qatar-1b

    Science.gov (United States)

    Püsküllü, Ç.; Soydugan, F.; Erdem, A.; Budding, E.

    2017-08-01

    New photometric follow-up observations of transitting 'hot Jupiters' TrES-3b and Qatar-1b are presented. Weighted mean values of the solutions of light curves in R-filter for both planetary systems are reported and compared with the previous results. The transit light curves were analysed using the WINFITTER code. The physical properties of the planets were estimated. The planet radii are found to be Rp = 1.381 ± 0.033RJ for TrES-3b and Rp = 1.142 ± 0.025RJ for Qatar-1b. Transit times and their uncertainties were also determined and a new linear ephemeris was computed for both systems. Analysis of transit times showed that a significant signal could not be determined for TrES-3b, while weak evidence was found for Qatar-1b, which might be tested using more precise future transit times.

  3. A TEM study on the crystallization of amorphous Fe 73Si 3B 24 alloys

    Science.gov (United States)

    Lijun, Wu; Lihua, Zhao; Wangyu, Hu; Lingling, Wang; Juemin, Xiao

    1994-02-01

    The crystallization of Fe 73Si 3B 24 amorphous alloys has been studied by transmission electron microscopy (TEM). A metastable phase P has been found and determined by a double tilting method in TEM. This phase belongs to a primitive tetragonal lattice with a = 0.62 nm and c = 1.43 nm. Its probable space group is P4nc or P4/mnc. In the Fe 2B phase, we found threefold twins which are rotated 120° around the [ overline1 1 0] axis. The crystallization process of this alloy is suggested to be Amorphous→Amorphous + α-Fe(Si) + Fe 3B + P → α-Fe(Si) + Fe 3B + Fe 2B → α-Fe(Si) + Fe 2B.

  4. Thieno[2,3-b]pyridines--a new class of multidrug resistance (MDR) modulators.

    Science.gov (United States)

    Krauze, Aivars; Grinberga, Signe; Krasnova, Laura; Adlere, Ilze; Sokolova, Elina; Domracheva, Ilona; Shestakova, Irina; Andzans, Zigmars; Duburs, Gunars

    2014-11-01

    To identify new potent multidrug resistance modulators, we have synthesized a series of novel thieno[2,3-b]pyridines and furo[2,3-b]pyridines, and examined their structure-activity relationships. All synthesized compounds were tested to determine BCRP1, P-gp, and MRP1 inhibitor activity, and most potent MDR modulators were also screened for their toxicity, cytotoxicity and Ca(2+) channel antagonist activity. Among these compounds, thieno[2,3-b]pyridine (6r) was found to exhibit a potent P-gp inhibitory action with EC50 = 0.3 ± 0.2 μM, MRP1 inhibitory action with EC50 = 1.1 ± 0.1 μM and BCRP1 inhibitory action with EC50 = 0.2 ± 0.05 μM and may represent suitable candidate for further pharmacological studies.

  5. Adenovirus vector expressing mda-7 selectively kills hepatocellular carcinoma cell line Hep3B

    Institute of Scientific and Technical Information of China (English)

    Xin-Bo Xue; Kun Chen; Cong-Jun Wang; Jian-Wei Zheng; Yuan Yu; Zhi-Hai Peng; Zai-De Wu

    2008-01-01

    BACKGROUND: Melanoma differentiation associated gene-7 (mda-7) is a novel tumor suppressor gene, which has suppressor activity in a broad spectrum of human cancer cells both in vitro and in vivo through activation of various intracellular signaling pathways. In this study, we investigated the potential effect of mda-7 on human hepatocellular carcinoma (HCC) in vitro. METHODS: Cells from the human HCC cell line Hep3B and the human liver cell line L-02 were assigned to three groups. One was cultured in Dulbecco's modiifed Eagle's medium without serum (control). The others were transfected with adenovirus expressing the mda-7 gene (Ad.mda-7) or adenovirus vector serving as negative control (Ad.vec). The expression of MDA-7 and Bcl-2 proteins in Hep3B and L-02 cells was conifrmed by the reverse transcriptase-polymerase chain reaction and enzyme-linked immunosorbent assay. The methyl thiazolyl tetrazolium colorimetric assay and lfow cytometry were used to assess tumor cell proliferation and the cell cycle. Hoechst and Annexin-V/propidium iodide staining were used to study mda-7 gene expression in Hep3B and L-02 cells. The expression of MDA-7, Bcl-2 and Bax proteins were detected by Western blotting. RESULTS: The mda-7 gene was expressed in Hep3B and L-02 cells. The protein concentrations of MDA-7 in supernatants were 790 and 810 pg/ml, respectively. mda-7 induced Hep3B growth suppression and apoptosis, compared with Ad.mda-7 and control (P CONCLUSIONS: mda-7 selectively induces growth inhibi-tion and apoptosis in the HCC cell line Hep3B but not in the normal liver cell line L-02 via downregulating the anti-apoptosis protein Bcl-2. It could be an ideal gene for gene therapy in HCC.

  6. Oak Ridge National Laboratory Technology Logic Diagram. Volume 3, Technology evaluation data sheets: Part B, Dismantlement, Remedial action

    Energy Technology Data Exchange (ETDEWEB)

    1993-09-01

    The Oak Ridge National Laboratory Technology Logic Diagram (TLD) was developed to provide a decision support tool that relates environmental restoration (ER) and waste management (WM) problems at Oak Ridge National Laboratory (ORNL) to potential technologies that can remediate these problems. The TLD identifies the research, development, demonstration testing, and evaluation needed to develop these technologies to a state that allows technology transfer and application to decontamination and decommissioning (D&D), remedial action (RA), and WM activities. The TLD consists of three fundamentally separate volumes: Vol. 1, Technology Evaluation; Vol. 2, Technology Logic Diagram and Vol. 3, Technology EvaLuation Data Sheets. Part A of Vols. 1 and 2 focuses on RA. Part B of Vols. 1 and 2 focuses on the D&D of contaminated facilities. Part C of Vols. 1 and 2 focuses on WM. Each part of Vol. 1 contains an overview of the TM, an explanation of the problems facing the volume-specific program, a review of identified technologies, and rankings of technologies applicable to the site. Volume 2 (Pts. A. B. and C) contains the logic linkages among EM goals, environmental problems, and the various technologies that have the potential to solve these problems. Volume 3 (Pts. A. B, and C) contains the TLD data sheets. This volume provides the technology evaluation data sheets (TEDS) for ER/WM activities (D&D, RA and WM) that are referenced by a TEDS code number in Vol. 2 of the TLD. Each of these sheets represents a single logic trace across the TLD. These sheets contain more detail than is given for the technologies in Vol. 2.

  7. Oak Ridge National Laboratory Technology Logic Diagram. Volume 3, Technology evaluation data sheets: Part C, Robotics/automation, Waste management

    Energy Technology Data Exchange (ETDEWEB)

    1993-09-01

    The Oak Ridge National Laboratory Technology Logic Diagram (TLD) was developed to provide a decision support tool that relates environmental restoration (ER) and waste management (WM) problems at Oak Ridge National Laboratory (ORNL) to potential technologies that can remediate these problems. The TLD identifies the research, development, demonstration testing, and evaluation needed to develop these technologies to a state that allows technology transfer and application to decontamination and decommissioning (D&D), remedial action (RA), and WM activities. The TLD consists of three fundamentally separate volumes: Vol. 1, Technology Evaluation; Vol. 2, Technology Logic Diagram and Vol. 3, Technology EvaLuation Data Sheets. Part A of Vols. 1 and 2 focuses on RA. Part B of Vols. 1 and 2 focuses on the D&D of contaminated facilities. Part C of Vols. 1 and 2 focuses on WM. Each part of Vol. 1 contains an overview of the TM, an explanation of the problems facing the volume-specific program, a review of identified technologies, and rankings of technologies applicable to the site. Volume 2 (Pts. A. B. and C) contains the logic linkages among EM goals, environmental problems, and the various technologies that have the potential to solve these problems. Volume 3 (Pts. A. B, and C) contains the TLD data sheets. This volume provides the technology evaluation data sheets (TEDS) for ER/WM activities (D&D, RA and WM) that are referenced by a TEDS code number in Vol. 2 of the TLD. Each of these sheets represents a single logic trace across the TLD. These sheets contain more detail than is given for the technologies in Vol. 2.

  8. Germline Variation in HSD3B1 as a Novel Biomarker in Prostate Cancer

    Science.gov (United States)

    2016-10-01

    dx.doi.org/10.1016/S1470-2045(16)30227-3 Articles HSD3B1 and resistance to androgen-deprivation therapy in prostate cancer : a retrospective...et al. Intermittent versus continuous androgen deprivation in prostate cancer . N Engl J Med 2013; 368: 1314–25. 20 Articles www.thelancet.com...AWARD NUMBER: W81XWH-15-1-0503 TITLE: Germline Variation in HSD3B1 as a Novel Biomarker in Prostate Cancer PRINCIPAL INVESTIGATOR: Jason

  9. Redetermination of the perovskite-type compound YRh3B revealing a Rh deficiency

    Directory of Open Access Journals (Sweden)

    Humihiko Takei

    2008-10-01

    Full Text Available In contrast with previous structural studies of ytterbium trirhodium boride, YbRh3B, that suggest a boron deficiency, the current redetermination of the crystal structure of YbRh3B revealed instead a rhodium deficiency with a refined composition of YbRh2.67 (2B. In the ABX3 perovskite-type structure, Yb, B and Rh are located on the A, B and X positions, respectively, with site symmetries of moverline{3}m for the A and B sites, and 4/mm.m for the X site.

  10. Analyzing the Effects of Nitrogen Deficiency on the PHB Production of Methylosinus trichosporium OB3b

    Science.gov (United States)

    Kyauk, E.

    2011-12-01

    Polyhydroxybutyrate (PHB) is a biodegradable thermoplastic that is produced by various microorganisms. Because of its potential to replace conventional plastics, it has been closely researched in the past few years. Methanotrophic bacteria, bacteria that consume methane, produce this bioplastic when it lacks certain nutrients. The utilization of methane to produce PHB shows much promise as methane is a cheap, plentiful gas. In this study, we observed the methanotroph, Methylosinus trichosporium OB3b , and its yield of PHB in the absence of nitrogen. The optical density of Methylosinus trichosporium OB3b was measured in order to observe cell growth and PHB production patterns over a 48 hour period.

  11. Microwave assisted convenient synthesis of quino[2,3-b][1,5]benzoxazepines

    Institute of Scientific and Technical Information of China (English)

    Swapnil S.Sonar; Sandip A.Sadaphal; Shivaji S.Pawar; Bapurao B.Shingate; Murlidhar S.Shingare

    2009-01-01

    A convenient synthesis of quino[2,3-b][1,5]benzoxazepines from substituted 2-chloroquinoline-3-carbaldehyde and 2-hydroxy-aniline by using stable,non-toxic and inexpensive catalyst 1,8-diazabicyclo-undecan-7-ene (DBU)/silica gel is described.This method affords the quino[2,3-b][1,5]benzoxazepines under the influence of microwave irradiation (360 W) in solvent-free conditions within short reaction times (2-3 min),giving high yields of products (93-96%).

  12. fMRI assessment of somatotopy in human Brodmann area 3b by electrical finger stimulation.

    Science.gov (United States)

    Kurth, R; Villringer, K; Mackert, B M; Schwiemann, J; Braun, J; Curio, G; Villringer, A; Wolf, K J

    1998-01-26

    Functional magnetic resonance imaging (fMRI) is capable of detecting focal brain activation induced by electrical stimulation of single fingers in human subjects. In eight subjects somatotopic arrangement of the second and fifth finger was found in Brodmann area 3b of the primary somatosensory cortex. In four subjects the representation area of the second finger was located lateral and inferior to the fifth finger; in one subject the somatotopy was reversed. In three subjects representation areas of the two fingers in Brodmann area 3b were found overlapping. Additional activated areas were found on the crown of ipsilateral and contralateral postcentral gyrus (Brodmann areas 1 and 2) and posterior parietal cortex.

  13. Spectroscopic evidence against Rh 4d itinerant ferromagnetism in CeRh3B2

    Science.gov (United States)

    Sampathkumaran, E. V.; Kaindl, G.; Laubschat, C.; Krone, W.; Wortmann, G.

    1985-03-01

    Valence-band photoemission and IIII-edge x-ray absorption studies were performed on RRh3B2 (R = La, Ce, and Pr) compounds in order to explore the origin of the anomalously high magnetic ordering temperature of CeRh3B2. From the low density of Rh 4d states at EF and the approximate trivalency of Ce, itinerant magnetism in the Rh 4d band can be excluded. Instead, the magnetism is proposed to originate from Ce moments, enhanced by 4f hybridization with Rh d orbitals.

  14. Fine mapping of a major QTL for grain yield on chromosome 3B of durum wheat

    OpenAIRE

    Graziani, Marta

    2012-01-01

    In durum wheat, two major QTL for grain yield (Qyld.idw-2B and Qyld.idw-3B) and related traits were identified in a recombinant population derived from Kofa and Svevo (Maccaferri et al. 2008). To further investigate the genetic and physiological basis of allelic variation for this important trait, the fine mapping of Qyld.idw-2B e Qyld.idw-3B was done during the PhD. In this regard, new molecular markers were added to increase the map resolution in the target interval. For Qyld.idw-2B ...

  15. miR-148 targets human DNMT3b protein coding region

    OpenAIRE

    Duursma, Anja M.; Kedde, Martijn; Schrier, Mariette; le Sage, Carlos; Agami, Reuven

    2008-01-01

    MicroRNAs (miRNAs) are small noncoding RNA molecules of 20–24 nucleotides that regulate gene expression. In animals, miRNAs form imperfect interactions with sequences in the 3′ Untranslated region (3′UTR) of mRNAs, causing translational inhibition and mRNA decay. In contrast, plant miRNAs mostly associate with protein coding regions. Here we show that human miR-148 represses DNA methyltransferase 3b (Dnmt3b) gene expression through a region in its coding sequence. This region is evolutionary ...

  16. VolP开始走向实用

    Institute of Scientific and Technical Information of China (English)

    Russ Anensman

    2006-01-01

    <正>德州仪器(TI)的Tom Flanagan已为早期基于IP协议的语音(VolP)技术天花乱坠的广告宣传进入实用等待了10年之久。TI VoIP部技术战略总监Flanagan说:“我不可能告诉你实现这一点需要多少时间。”

  17. 76 FR 14419 - Exercise of Authority Under Section 212(d)(3)(B)(i) of the Immigration and Nationality Act

    Science.gov (United States)

    2011-03-16

    ... SECURITY Office of the Secretary Exercise of Authority Under Section 212(d)(3)(B)(i) of the Immigration and...(d)(3)(B)(i) of the Immigration and Nationality Act (INA), 8 U.S.C. 1182(d)(3)(B)(i), as amended, as.... Citizenship and Immigration Services (USCIS), in consultation with U.S. Immigration and Customs...

  18. 76 FR 14418 - Exercise of Authority Under Section 212(d)(3)(B)(i) of the Immigration and Nationality Act

    Science.gov (United States)

    2011-03-16

    ... SECURITY Office of the Secretary Exercise of Authority Under Section 212(d)(3)(B)(i) of the Immigration and...(d)(3)(B)(i) of the Immigration and Nationality Act (INA), 8 U.S.C. 1182(d)(3)(B)(i), as amended, as... determination will be made by U.S. Citizenship and Immigration Services (USCIS), in consultation with...

  19. 77 FR 41795 - Exercise of Authority Under Section 212(d)(3)(B)(i) of the Immigration and Nationality Act

    Science.gov (United States)

    2012-07-16

    ... SECURITY Office of the Secretary Exercise of Authority Under Section 212(d)(3)(B)(i) of the Immigration and...(d)(3)(B)(i) of the Immigration and ] Nationality Act (INA), 8 U.S.C. 1182(d)(3)(B)(i), as amended.... Implementation of this determination will be made by U.S. Citizenship and Immigration Services (USCIS),...

  20. From Editor vol 11, No.3

    Directory of Open Access Journals (Sweden)

    Ugur Demiray

    2010-07-01

    Full Text Available Greetings Dear readers of TOJDE,TOJDE is appeared on your screen now as Volume 11, Number: 3. In this issue it is published 3 notes for Editor, 12 articles, 2 book reviews. And this time, 32 authors from 10 different countries are placed. These published articles are from Barbados, Ghana, Iran, Malaysia, Pakistan, Spain, Turkey, United Kingdom, UAE and USA.“Developing and Validation A Usability Evaluation Tools For Distance Education Websites:Persian Version” has sent as Notes for editor section of TOJDE from Iran and written by Soheila HAFEZI, Ahmad FARAHI from Payame Noor University and Soheil Najafi MEHRI, Hosein MAHMOODI from Baqiyatallah Medical Sciences University, Tehran.. Their paper involves that he content validity index was measured by set of ten experts, who evaluated each item individually. According to CVI, the final version of instrument was composed of 40 questions divided into 8 domains: Navigation, Functionality, Feedback, Control, Language, Consistency, Error prevention and correction, and Visual clarity. CVI score for each phrase was more than 0.75. According to our findings, this instrument has enough validity to apply in evaluation usability of educational websites of Persian distance education websites. However, instrument reliability can be measured in further study. The second notes for editor is titled as “A Critical Analysis Of Managerial Skills Competencies Of Secondary School Heads Trained Through Distance Mode of Allama Iqbal Open University” which is written by Muhammad AKHLAQ from Preston University, Islamabad, PAKISTAN and SHAZIA MUNAWAR SULEHRI from Ministry of Education, PAKISTAN. They mentioned in their paper to analyze the managerial skills competencies of secondary schools heads trained through distance mode of education in Pakistan. For this purpose a sample 300 secondary school teachers and 100 secondary schools head-teachers trained through distance mode and working in the Federal Government

  1. From Editor vol 11, No.4

    Directory of Open Access Journals (Sweden)

    Ugur Demiray

    2010-10-01

    Full Text Available Greetings Dear readers of TOJDE,TOJDE is appeared on your screen now as Volume 11, Number: 4. In this issue it is published 4 notes for Editor, 12 articles, 2 book and one conference reviews. And this time, 27 authors from 10 different countries are placed. These published articles are from Bangldesh, Greece, India, Israel, Malaysia, Nigeria, Portugal, Singapore, Turkey and USA.The first Notes for editor arrived from USA, written by Kevin YEE and Jace HARGIS. They focused on PREZI: A Different Way to Present. Prezi represents the first step toward other visual tools that are not, strictly speaking, and presentations at all, but may yet find uses in classrooms. Browser-based programs that allow for concept mapping and brainstorming (sometimes with drawing and even inter-user chat functionality approximate some of prezi’s best features yet stand on their own as organizing tools that provide inspiration for the users. Examples include bubble.us, Thinkature, Mindmeister, and Graphic Organizer. The second notes for editor is titled as “Investigating the Factor Structure ıf The Blog Attitude Scale” which is written by Zahra SHAHSAVAR, Tan Bee HOON, S. Vahid ARYADOUST from Malaysia. Their study reports the design and development of a blog attitude scale (BAS. In exploratory factor analysis, three factors were discovered: blog anxiety, blog desirability, and blog self-efficacy; 14 items were excluded. The extracted items were subjected to a confirmatory factor analysis which lent further support to the BAS underpinning structure. “Implementation of An Online Teacher Assessment/Appraisal In Technical Education Institution: A Case Study” is the third paper for “Notes for Editor” section of TOJDE’s in this issue. It has written by Sraboni MANDAL, Dr. SANJAY, Dajnish SHRIVASTAVA from National Institute of Technology, Jamshedpur, INDIA. The purpose to discusses a case study of implementation of teacher appraisal system which initially non

  2. Johnston Atoll Site 3B-P 7/1/2000 36-37M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 3B-P (16 45.260N, 169 31.039W), between 36 and 37 meters along a permanent transect.

  3. Johnston Atoll Site 3B-P 7/1/2000 1-2M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 3B-P (16 45.260N, 169 31.039W), between 1 and 2 meters along a permanent transect.

  4. The superfamily of C3b/C4b-binding proteins

    DEFF Research Database (Denmark)

    Kristensen, Torsten; D'Eustachio, P; Ogata, R T

    1987-01-01

    The determination of primary structures by amino acid and nucleotide sequencing for the C3b-and/or C4b-binding proteins H, C4BP, CR1, B, and C2 has revealed the presence of a common structural element. This element is approximately 60 amino acids long and is repeated in a tandem fashion, commenci...

  5. Kinetics of Chlorinated Hydrocarbon Degradation by Methylosinus trichosporium OB3b and Toxicity of Trichloroethylene

    NARCIS (Netherlands)

    Oldenhuis, Roelof; Oedzes, Johannes Y.; Waarde, Jacob J. van der; Janssen, Dick B.

    1991-01-01

    The kinetics of the degradation of trichloroethylene (TCE) and seven other chlorinated aliphatic hydrocarbons by Methylosinus trichosporium OB3b were studied. All experiments were performed with cells grown under copper stress and thus expressing soluble methane monooxygenase. Compounds that were re

  6. Degradation of Chlorinated Aliphatic Hydrocarbons by Methylosinus trichosporium OB3b Expressing Soluble Methane Monooxygenase

    NARCIS (Netherlands)

    Oldenhuis, Roelof; Vink, Ruud L.J.M.; Janssen, Dick B.; Witholt, Bernard

    1989-01-01

    Degradation of trichloroethylene (TCE) by the methanotrophic bacterium Methylosinus trichosporium OB3b was studied by using cells grown in continuous culture. TCE degradation was a strictly cometabolic process, requiring the presence of a cosubstrate, preferably formate, and oxygen. M. trichosporium

  7. The Mad1-Sin3B interaction involves a novel helical fold.

    NARCIS (Netherlands)

    Spronk, C.A.E.M.; Tessari, M.; Kaan, A.M.; Jansen, J.F.; Vermeulen, M.; Stunnenberg, H.G.; Vuister, G.W.

    2000-01-01

    Sin3A or Sin3B are components of a corepressor complex that mediates repression by transcription factors such as the helix-loop-helix proteins Mad and Mxi. Members of the Mad/Mxi family of repressors play important roles in the transition between proliferation and differentiation by down-regulating

  8. Johnston Atoll Site 3B-P 7/1/2000 2-3M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 3B-P (16 45.260N, 169 31.039W), between 2 and 3 meters along a permanent transect.

  9. Johnston Atoll Site 3B-P 7/1/2000 35-36M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 3B-P (16 45.260N, 169 31.039W), between 35 and 36 meters along a permanent transect.

  10. Johnston Atoll Site 3B-P 7/1/2000 6-7M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 3B-P (16 45.260N, 169 31.039W), between 6 and 7 meters along a permanent transect.

  11. Johnston Atoll Site 3B-P 7/1/2000 11-12M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 3B-P (16 45.260N, 169 31.039W), between 11 and 12 meters along a permanent transect.

  12. Johnston Atoll Site 3B-P 7/1/2000 5-6M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 3B-P (16 45.260N, 169 31.039W), between 5 and 6 meters along a permanent transect.

  13. Johnston Atoll Site 3B-P 7/1/2000 42-43M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 3B-P (16 45.260N, 169 31.039W), between 42 and 43 meters along a permanent transect.

  14. Johnston Atoll Site 3B-P 7/1/2000 16-17M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 3B-P (16 45.260N, 169 31.039W), between 16 and 17 meters along a permanent transect.

  15. Johnston Atoll Site 3B-P 7/1/2000 7-8M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 3B-P (16 45.260N, 169 31.039W), between 7 and 8 meters along a permanent transect.

  16. Johnston Atoll Site 3B-P 7/1/2000 3-4M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 3B-P (16 45.260N, 169 31.039W), between 3 and 4 meters along a permanent transect.

  17. Johnston Atoll Site 3B-P 7/1/2000 30-31M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 3B-P (16 45.260N, 169 31.039W), between 30 and 31 meters along a permanent transect.

  18. Magnetism and superconductivity in the system Ce1-xLaxRh3B2

    Science.gov (United States)

    Malik, S. K.; Umarji, A. M.; Shenoy, G. K.; Aldred, A. T.; Niarchos, D. G.

    1985-10-01

    The compound CeRh3B2 is magnetically ordered with a low saturation moment of ~0.4 μB per formula unit and an unusually large Curie temperature of 115 K while isostructural LaRh3B2 is superconducting with a transition temperature of ~2.3 K. Magnetic and superconducting studies on the series of compounds Ce1-xLaxRh3B2 reveal that the magnetic state persists for 0<=x<=0.8. The magnetic moment per Ce ion goes through a maximum and the Curie temperature decreases as La is substituted for Ce. On the La-rich side, superconductivity is destroyed even with 1% replacement of La by Ce, indicating a strong coupling of Ce 4f moments with conduction-electron spins. The temperature dependence of the upper critical field of LaRh3B2 has been measured and is discussed in terms of Werthammer-Helfand-Hohenberg theory.

  19. Measurement of local moment on Ce in CeRh3B2

    Science.gov (United States)

    Devare, S. H.; Devare, H. G.

    1987-03-01

    We have employed the TDPAC technique to measure the paramagnetic enhancement factor β of140Ce in CeRh3B2 in the temperature range 300 K down to 115 K. Our measurements show that above Tc=115 K, the trend for β(T) closely follows that for trivalent cerium.

  20. Johnston Atoll Site 3B-P 7/1/2000 9-10M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 3B-P (16 45.260N, 169 31.039W), between 9 and 10 meters along a permanent transect.

  1. Physical Implementation of the Eight-Core Godson-3B Microprocessor

    Institute of Scientific and Technical Information of China (English)

    Ru Wang; Wei-Wu Hu; Bao-Xia Fan; Liang Yang; Yan-Ping Gao; Dong Liu; Bin Xiao; Jiang-Mei Wang; Yi-Fu Zhang; Hong Wang

    2011-01-01

    The Godson-3B processor is a powerful processor designed for high performance servers including Dawning Servers. It offers significantly improved performance over previous Godsono3 series CPUs by incorporating eight CPU cores and vector computing units. It contains 582.6 M transistors within 300 mm2 area in 65 nm technology and is implemented in parallel with full hierarchical design flows. In Godson-3B, advanced clock distribution mechanisms including GALS (Globally Asynchronous Locally Synchronous) and clock mesh are adopted to obtain an OCV tolerable clock network. Custom-designed de-skew modules are also implemented to afford further latency balance after fabrication. The power reduction of Godson-3B is maintained by MLMM (Multi Level Multi Mode) clock gating and multi-threshold-voltage cells substitution schemes. The highest frequency of Godson-3B is 1.05 GHz and the peak performance is 128 GFlops (double-precision) or 256 GFlops (single-precision) with 40 W power consumption.

  2. The 46359CT polymorphism of DNMT3B is associated with the risk of cervical cancer.

    Science.gov (United States)

    Hernández-Sotelo, Daniel; García-Aguilar, Rubén; Castro-Coronel, Yaneth; Magaña, Jonathan J; Leyva-Vazquez, Marco Antonio; Alarcón-Romero, Luz del Carmen; López-Bayghen, Esther; Illades-Aguiar, Berenice

    2013-07-01

    Abnormal methylation is related to cancer development. Since DNMT3B is an enzyme that modulates genomic methylation, we hypothesized that genetic variants of the promoter DNMT3B may be associated with an increased risk of developing cervical cancer. Our aim was to investigate the association between -579GT and 46359CT polymorphisms of DNMT3B and cervical cancer, high-grade squamous intraepithelial lesions (HSIL), and low-grade squamous intraepithelial lesions (LSIL). Samples from 200 healthy women and 130 women with squamous intraepithelial lesions (70 with cervical cancer, 30 with HSIL, and 30 with LSIL) were analyzed. Polymorphism genotyping was performed using PCR and restriction fragment length polymorphism. The -579GT polymorphism was not associated with cervical cancer, HSIL, or LSIL. The CT genotype of 46359CT polymorphism was significantly associated with cervical cancer risk (OR 8.75, CI 1.27-374.1), whereas the TT genotype was associated with a significantly decreased risk of HSIL (OR 0.66, CI 0.01-0.32) and LSIL (OR 0.11, CI 0.026-0.45). Our results suggest that genotyping the 46359CT polymorphism in DNMT3B may help identify women who are genetically susceptible to cervical cancer development. Additional studies with larger sample sizes are necessary to confirm our findings.

  3. Genomic profiling of DNA methyltransferases reveals a role for DNMT3B in genic methylation.

    Science.gov (United States)

    Baubec, Tuncay; Colombo, Daniele F; Wirbelauer, Christiane; Schmidt, Juliane; Burger, Lukas; Krebs, Arnaud R; Akalin, Altuna; Schübeler, Dirk

    2015-04-09

    DNA methylation is an epigenetic modification associated with transcriptional repression of promoters and is essential for mammalian development. Establishment of DNA methylation is mediated by the de novo DNA methyltransferases DNMT3A and DNMT3B, whereas DNMT1 ensures maintenance of methylation through replication. Absence of these enzymes is lethal, and somatic mutations in these genes have been associated with several human diseases. How genomic DNA methylation patterns are regulated remains poorly understood, as the mechanisms that guide recruitment and activity of DNMTs in vivo are largely unknown. To gain insights into this matter we determined genomic binding and site-specific activity of the mammalian de novo DNA methyltransferases DNMT3A and DNMT3B. We show that both enzymes localize to methylated, CpG-dense regions in mouse stem cells, yet are excluded from active promoters and enhancers. By specifically measuring sites of de novo methylation, we observe that enzymatic activity reflects binding. De novo methylation increases with CpG density, yet is excluded from nucleosomes. Notably, we observed selective binding of DNMT3B to the bodies of transcribed genes, which leads to their preferential methylation. This targeting to transcribed sequences requires SETD2-mediated methylation of lysine 36 on histone H3 and a functional PWWP domain of DNMT3B. Together these findings reveal how sequence and chromatin cues guide de novo methyltransferase activity to ensure methylome integrity.

  4. Angiogenin contributes to bladder cancer tumorigenesis by DNMT3b-mediated MMP2 activation.

    Science.gov (United States)

    Peres, Rafael; Furuya, Hideki; Pagano, Ian; Shimizu, Yoshiko; Hokutan, Kanani; Rosser, Charles J

    2016-07-12

    Epigenetic-mediated gene activation/silencing plays a crucial role in human tumorigenesis. Eliciting the underlying mechanism behind certain epigenetic changes is essential for understanding tumor biology. Previous studies in human cancers revealed an unrecognized interplay between Angiogenin (ANG) and matrix metalloproteinase-2 (MMP2) leading to pronounced tumorigenesis. Here we provide multiple lines of evidence further indicating ANG oncogenic potential. ANG expression resulted in the hypomethylated state of the MMP2 gene, which led to increased gene expression of MMP2. More than that, our global DNA methylation microarray analysis showed that gene manipulation of ANG affected a variety of pathways, such as cell migration, angiogenesis and specifically, tumor suppressor genes. Mechanistically, ANG negatively regulated DNA methyltransferase 3b (DNMT3b) enzymatic activity by down-regulating its expression and inhibiting its recruitment to the MMP2 promoter. Consistent with this, ANG-MMP2 overexpression and DNMT3b underexpression correlated with reduction in disease free survival of human bladder cancer patients. Together, the results continue to establish ANG as an oncoprotein and further reveal that ANG contributes to oncogenesis by the activation of MMP2 through modulation of DNMT3b functions.

  5. Macrophage cytokines render WEHI-3B tumor cells susceptible to cytostasis by prostaglandins

    NARCIS (Netherlands)

    S. Ben-Efraim; C.J.A.M. Tak (Corné); I.L. Bonta

    1990-01-01

    markdownabstractAbstract The growth of the murine myelomonocytic leukemia tumor, WEHI-3B, has been shown to be inhibited by a two-step treatment: first, incubation for one hour with either interleukin-1 (human recombinant IL-1α or tumor necrosis factor (human recombinant TNF-α); second, subsequent

  6. Endogenous APOBEC3B restricts LINE-1 retrotransposition in transformed cells and human embryonic stem cells.

    Science.gov (United States)

    Wissing, Silke; Montano, Mauricio; Garcia-Perez, Jose Luis; Moran, John V; Greene, Warner C

    2011-10-21

    Members of the APOBEC3 (A3) family of cytidine deaminase enzymes act as host defense mechanisms limiting both infections by exogenous retroviruses and mobilization of endogenous retrotransposons. Previous studies revealed that the overexpression of some A3 proteins could restrict engineered human Long INterspersed Element-1 (LINE-1 or L1) retrotransposition in HeLa cells. However, whether endogenous A3 proteins play a role in restricting L1 retrotransposition remains largely unexplored. Here, we show that HeLa cells express endogenous A3B and A3C, whereas human embryonic stem cells (hESCs) express A3B, A3C, A3DE, A3F, and A3G. To study the relative contribution of endogenous A3 proteins in restricting L1 retrotransposition, we first generated small hairpin RNAs (shRNAs) to suppress endogenous A3 mRNA expression, and then assessed L1 mobility using a cell-based L1 retrotransposition assay. We demonstrate that in both HeLa and hESCs, shRNA-based knockdown of A3B promotes a ∼2-3.7-fold increase in the retrotransposition efficiency of an engineered human L1. Knockdown of the other A3s produced no significant increase in L1 activity. Thus, A3B appears to restrict engineered L1 retrotransposition in a broad range of cell types, including pluripotent cells.

  7. Data of evolutionary structure change: 1JJ3B-2G4NF [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1JJ3B-2G4NF 1JJ3 2G4N B F KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFN... HHHH - GGGG -- EVID>EVID> 0 2G4N F 2G4NF IVQNN...639686584473 EVID> 1 2G4N... F 2G4NF KKILD-KVGIN

  8. Intracellular glutathione regulates Andrographolide-induced cytotoxicity on hepatoma Hep3B cells.

    Science.gov (United States)

    Ji, Lili; Shen, Kaikai; Liu, Jun; Chen, Ying; Liu, Tianyu; Wang, Zhengtao

    2009-01-01

    Andrographolide (ANDRO), a diterpenoid lactone isolated from the traditional herbal plant Andrographis paniculata, was reported to induce apoptosis in hepatoma Hep3B cells in our previous study (Ji LL, Liu TY, Liu J, Chen Y, Wang ZT. Andrographolide inhibits human hepatoma-derived Hep3B cells growth through the activation of c-Jun N-terminal kinase. Planta Med 2007; 73: 1397-1401). The present investigation was carried out to observe whether cellular reduced glutathione (GSH) plays important roles in ANDRO-induced apoptosis. ANDRO initially increased intracellular GSH levels which then decreased later, while inhibition of cellular GSH synthesis by L-Buthionine-(S,R)-sulfoximine (BSO) augmented ANDRO-induced cytotoxicity and apoptosis in Hep3B cells. On the other hand, the thiol antioxidant dithiothreitol (DTT) rescued ANDRO-depleted cellular GSH, and abrogated ANDRO-induced cytotoxicity and apoptosis. Furthermore, BSO pretreatment augmented ANDRO-decreased expression of antioxidant protein thioredoxin 1 (Trx1), while DTT reversed this decrease. Further results showed that ANDRO increased the activity of the GSH-related antioxidant enzyme glutathione peroxidase (GPx) and the production of intracellular reactive oxygen species (ROS). Taken together, this study demonstrates that the intracellular redox system plays important roles in regulating the cytotoxicity of ANDRO on hepatoma Hep3B cells.

  9. Genetic variation of the Fc gamma receptor 3B gene and association with rheumatoid arthritis.

    Directory of Open Access Journals (Sweden)

    Rute B Marques

    Full Text Available BACKGROUND: Fc gamma receptors (FcγRs play a crucial role in immunity by linking IgG antibody-mediated responses with cellular effector and regulatory functions. Genetic variants in these receptors have been previously identified as risk factors for several chronic inflammatory conditions. The present study aimed to investigate the presence of copy number variations (CNVs in the FCGR3B gene and its potential association with the autoimmune disease rheumatoid arthritis (RA. METHODOLOGY/PRINCIPAL FINDINGS: CNV of the FCGR3B gene was studied using Multiplex Ligation Dependent Probe Amplification (MLPA in 518 Dutch RA patients and 304 healthy controls. Surprisingly, three independent MLPA probes targeting the FCGR3B promoter measured different CNV frequencies, with probe#1 and #2 measuring 0 to 5 gene copies and probe#3 showing little evidence of CNV. Quantitative-PCR correlated with the copy number results from MLPA probe#2, which detected low copy number (1 copy in 6.7% and high copy number (≥3 copies in 9.4% of the control population. No significant difference was observed between RA patients and the healthy controls, neither in the low copy nor the high copy number groups (p-values = 0.36 and 0.71, respectively. Sequencing of the FCGR3B promoter region revealed an insertion/deletion (indel that explained the disparate CNV results of MLPA probe#1. Finally, a non-significant trend was found between the novel -256A>TG indel and RA (40.7% in healthy controls versus 35.9% in RA patients; P = 0.08. CONCLUSIONS/SIGNIFICANCE: The current study highlights the complexity and poor characterization of the FCGR3B gene sequence, indicating that the design and interpretation of genotyping assays based on specific probe sequences must be performed with caution. Nonetheless, we confirmed the presence of CNV and identified novel polymorphisms in the FCGR3B gene in the Dutch population. Although no association was found between RA and FCGR3B CNV, the

  10. Histone deacetylase inhibitors inducing human cervical cancer cell apoptosis by decreasing DNA-methyltransferase 3B

    Institute of Scientific and Technical Information of China (English)

    LIU Ning; ZHAO Li-jun; LI Xiao-ping; WANG Jian-liu; CHAI Guo-lin; WEI Li-hui

    2012-01-01

    Background Histone deacetylase (HDAC) inhibitors are a group of small chemical molecules that inhibit histone deacetylase.At cell level,HDAC inhibitors have multiple biological effects such as cell cycle arrest,apoptosis,cell differentiation and auotophagy.At molecular level,HDAC inhibitors cause histone and nonhistone acetylation and induce gene expression.HDAC inhibitors are widely used in cancer therapy because of its function of inducing apoptosis.However,the mechanisms of apoptosis effect are not fully understood.TSA is a classical HDAC inhibitor and widely used in epigenetic and anti-cancer research.In this study,we selected Trichostatin A (TSA) to investigate the mechanisms of HDAC inhibitors apoptotic effect on cancer cells.Methods Cervical cancer cell lines such as Hela,Caski and normal human keratinocyte line HaCaT were treated with various concentrations of TSA.Crystal violent assay and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay were performed to determine cell number.PARP cleavage and FITC-AnexinV were performed to determine apoptosis.DNA-methyltransferase (DNMT)1,DNMT3A and DNMT3B were determined by regular PCR,qPCR and Western Blotting.Small interfering RNA (SiRNAi) was used to knock down DNMT3B.Results HDAC inhibitors only induce cervical cancer cell apoptosis.At 1 μmol/L of TSA,86% of Hela cell and 76% of Caski went apoptosis.For normal cells,HDAC inhibitors have no cytotoxic effect at therapeutic dosage,(90.0±8.4)% of normal cell survive after treated with 1 μmol/L of TSA.We compared 1 μmol/L group with untreated control with t-test.There was no significance between 1 μmol/L group and untreated control for normal cell (P >0.05).HDAC inhibitors decreased DNMT3B in cancer cell but not in normal cell.Manually knock-down of DNMT3B induced Hela and Caski cell apoptosis.More than 99% of Hela and Caski cell went apoptosis after deprived of DNMT3B.Conclusions DNMT3B was essential to cervical cancer cell survival

  11. From the Editor Vol: 6 number 2

    Directory of Open Access Journals (Sweden)

    Ugur Demiray

    2005-04-01

    Full Text Available From the Editor Dear TOJDE’S Readers Time flies. Another quarter has begun. It is a great pleasure to welcome you to the 18th issue, the Volume: 6, Number: 2 of The Turkish Online Journal of Distance Education (TOJDE, of looking forward. TOJDE has been driving and shaping by our editorial board, reviewers, authors and even unbelievable readers. In the April 2005 issue, TOJDE publishes peer-reviewed distance education articles under four umbrellas: applications, feature topics, researches, and support systems. In addition, it publishes short and non-peer-reviewed book reviews and editorial letters. The main goal of TOJDE is to provide you with exclusive resources with the highest quality, and also to reach diverse audiences, as highly qualified individuals and researchers, around the world. Also, I am excited about that TOJDE is referenced as a course material by diverse institutions from the globe and very proud to share this news with you. You can see details in the news section. In this issue, it has been given place to two notes for editor submissions, eleven articles, two book reviews, 8 news, one journal about information and communication technology and one institution about technology integration via distance education named as RETA. And also interesting links deal with TOJDE take place in the literature. This issue's articles came from Canada, India, Malaysia, Nigeria, Turkey, United Kingdom and United States (according to alphabetical order. The first “Notes for Editors” is from Turkey on Using of Marketing Communication for Distance Education Institutions submitted by Dr. R. Ayhan YILMAZ, Anadolu University, Communication Sciences Faculty, Eskisehir, TURKEY. And second one arrived from India. It is titled as “Information and Communication Technology for Poverty Reduction” written by Motilal SHARMA. The first article came from Canada. It is entitled as “Understanding PISA” written by Dr. Stephen DOWNES. He mentions in his paper

  12. Expression of △DNMT3B Variants and Its Association with Estrogen/Progestogen Receptor Status in Breast Cancer

    Institute of Scientific and Technical Information of China (English)

    Ning-hong Liu; Lu Yang; Ming-lei Zhuo; Jie Wang; Shu-hang Wang; Jun Zhao; Hua Bai

    2009-01-01

    Objective: Our previous study has showed that (DNMT3B is the predominant form of DNMT3B in non-small cell lung cancer (NSCLC). In this study, we aimed to explore the expression patterns of the (DNMT3B variants in breast cancer and to identify whether the pattern was similar to that in NSCLC or not and its clinical significance.Methods: Expression of seven (DNMT3B variants in 59 breast cancer and the corresponding normal tissue was measured using RT-PCR. The correlations between the expressions of (DNMT3B variants and the clinical parameters including ER/PR status, clincopathologic feature and survivals were analyzed.Results: There were significant differences in the expression ratios of (DNMT3B1-7 variants between breast cancer tissues and normal tissues (P<0.001). The positive ratio of (DNMT3B1-7 variants were 66%, 71%, 17%, 51%, 76.2%, 50% and 61% in tumor tissue, respectively; while 16%, 8.4%, 3.38%, 3.38%, 11.8%, 13.5% and 5.08% in the corresponding normal tissue, which was different from the pattern of (DNMT3B1-7 expression in NSCLC (62%, 76%, 2.5%, 46%, 18%, 27% and 16% in tumor tissue, respectively; while 18%, 11%, 0%, 3.3%, 0%, 0% and 0% in normal lung tissue, respectively; P<0.0001). Expressions of (DNMT3B2, 3B4 and 3B7 were higher in the patients with negative estrogen receptor (ER) than those with positive estrogen receptor (P=0.035, P=0.0141 and P=0.0219, respectively). (DNMT3B7 expression was higher for the patients with negative progestogen receptor (PR) compared to those with positive progestogen receptor (P=0.0379). Expression ratio of (DNMT3B5 in stage III tumors is lower than that in stage I/II ones (P= 0.041). But we did not find any relation between the (DNMT3B variants and the patients' survival.Conclusion: The pattern of (DNMT3B variants in breast cancer is different from that in NSCLC. Expressions of (DNMT3B2, 3B4 and 3B7 are associated with estrogen receptors status. While (DNMT3B7 is associated with progestogen receptor. No relation between

  13. Zebrafish foxo3b negatively regulates canonical Wnt signaling to affect early embryogenesis.

    Directory of Open Access Journals (Sweden)

    Xun-wei Xie

    Full Text Available FOXO genes are involved in many aspects of development and vascular homeostasis by regulating cell apoptosis, proliferation, and the control of oxidative stress. In addition, FOXO genes have been showed to inhibit Wnt/β-catenin signaling by competing with T cell factor to bind to β-catenin. However, how important of this inhibition in vivo, particularly in embryogenesis is still unknown. To demonstrate the roles of FOXO genes in embryogenesis will help us to further understand their relevant physiological functions. Zebrafish foxo3b gene, an orthologue of mammalian FOXO3, was expressed maternally and distributed ubiquitously during early embryogenesis and later restricted to brain. After morpholino-mediated knockdown of foxo3b, the zebrafish embryos exhibited defects in axis and neuroectoderm formation, suggesting its critical role in early embryogenesis. The embryo-developmental marker gene staining at different stages, phenotype analysis and rescue assays revealed that foxo3b acted its role through negatively regulating both maternal and zygotic Wnt/β-catenin signaling. Moreover, we found that foxo3b could interact with zebrafish β-catenin1 and β-catenin2 to suppress their transactivation in vitro and in vivo, further confirming its role relevant to the inhibition of Wnt/β-catenin signaling. Taken together, we revealed that foxo3b played a very important role in embryogenesis and negatively regulated maternal and zygotic Wnt/β-catenin signaling by directly interacting with both β-catenin1 and β-catenin2. Our studies provide an in vivo model for illustrating function of FOXO transcription factors in embryogenesis.

  14. Correlations among PPAR, DNMT1, and DNMT3B Expression Levels and Pancreatic Cancer

    Directory of Open Access Journals (Sweden)

    Valerio Pazienza

    2012-01-01

    Full Text Available Emerging evidence indicates that peroxisome proliferator-activated receptor γ (PPARγ and DNA methyltransferases (DNMTs play a role in carcinogenesis. In this study we aimed to evaluate the expression of PPARγ, DNMT1, and DNMT3B and their correlation with clinical-pathological features in patients with pancreatic cancer (PC, and to define the effect of PPARγ activation on DNMTs expression in PC cell lines. qRT-PCR analysis showed that DNMT3B expression was downregulated in tumors compared to normal tissues (=0.03, whereas PPARγ and DNMT1 levels did not show significant alterations in PC patients. Expression levels between PPARγ and DNMT1 and between DNMT1 and DNMT3B were highly correlated (=0.008 and =0.05 resp.. DNMT3B overexpression in tumor tissue was positively correlated with both lymph nodes spreading (=0.046 and resection margin status (=0.04, and a borderline association with perineural invasion (=0.06 was found. Furthermore, high levels of DNMT3B expression were significantly associated with a lower mortality in the whole population (HR=0.485; 95%CI=0.262–0.895, =0.02 and in the subgroup of patients without perineural invasion (HR=0.314; 95%CI=0.130–0.758; =0.01, while such association was not observed in patients with tumor invasion into perineural structures (=0.70. In conclusion, in vitro and in vivo PPARγ and DNMTs appear interrelated in PC, and this interaction might influence cell phenotype and disease behavior.

  15. Correlations among PPARγ, DNMT1, and DNMT3B Expression Levels and Pancreatic Cancer.

    Science.gov (United States)

    Pazienza, Valerio; Tavano, Francesca; Benegiamo, Giorgia; Vinciguerra, Manlio; Burbaci, Francesca Paola; Copetti, Massimiliano; di Mola, Fabio Francesco; Andriulli, Angelo; di Sebastiano, Pierluigi

    2012-01-01

    Emerging evidence indicates that peroxisome proliferator-activated receptor γ (PPARγ) and DNA methyltransferases (DNMTs) play a role in carcinogenesis. In this study we aimed to evaluate the expression of PPARγ, DNMT1, and DNMT3B and their correlation with clinical-pathological features in patients with pancreatic cancer (PC), and to define the effect of PPARγ activation on DNMTs expression in PC cell lines. qRT-PCR analysis showed that DNMT3B expression was downregulated in tumors compared to normal tissues (P = 0.03), whereas PPARγ and DNMT1 levels did not show significant alterations in PC patients. Expression levels between PPARγ and DNMT1 and between DNMT1 and DNMT3B were highly correlated (P = 0.008 and P = 0.05 resp.). DNMT3B overexpression in tumor tissue was positively correlated with both lymph nodes spreading (P = 0.046) and resection margin status (P = 0.04), and a borderline association with perineural invasion (P = 0.06) was found. Furthermore, high levels of DNMT3B expression were significantly associated with a lower mortality in the whole population (HR = 0.485; 95%CI = 0.262-0.895, P = 0.02) and in the subgroup of patients without perineural invasion (HR = 0.314; 95%CI = 0.130-0.758; P = 0.01), while such association was not observed in patients with tumor invasion into perineural structures (P = 0.70). In conclusion, in vitro and in vivo PPARγ and DNMTs appear interrelated in PC, and this interaction might influence cell phenotype and disease behavior.

  16. Mutants for Drosophila Isocitrate Dehydrogenase 3b Are Defective in Mitochondrial Function and Larval Cell Death

    Science.gov (United States)

    Duncan, Dianne M.; Kiefel, Paula; Duncan, Ian

    2017-01-01

    The death of larval salivary gland cells during metamorphosis in Drosophila melanogaster has been a key system for studying steroid controlled programmed cell death. This death is induced by a pulse of the steroid hormone ecdysone that takes place at the end of the prepupal period. For many years, it has been thought that the ecdysone direct response gene Eip93F (E93) plays a critical role in initiating salivary gland cell death. This conclusion was based largely on the finding that the three “type” alleles of E93 cause a near-complete block in salivary gland cell death. Here, we show that these three mutations are in fact allelic to Idh3b, a nearby gene that encodes the β subunit of isocitrate dehydrogenase 3, a mitochondrial enzyme of the tricarboxylic acid (TCA) cycle. The strongest of the Idh3b alleles appears to cause a near-complete block in oxidative phosphorylation, as mitochondria are depolarized in mutant larvae, and development arrests early during cleavage in embryos from homozygous-mutant germline mothers. Idh3b-mutant larval salivary gland cells fail to undergo mitochondrial fragmentation, which normally precedes the death of these cells, and do not initiate autophagy, an early step in the cell death program. These observations suggest a close relationship between the TCA cycle and the initiation of larval cell death. In normal development, tagged Idh3b is released from salivary gland mitochondria during their fragmentation, suggesting that Idh3b may be an apoptogenic factor that functions much like released cytochrome c in mammalian cells. PMID:28104670

  17. Procesado mediante una ruta pulvimetalúrgica de materiales compuestos Mg-10Ti (% vol

    Directory of Open Access Journals (Sweden)

    Pérez, P.

    2005-12-01

    Full Text Available The present study deals with the mechanical characterization at temperatures between 25 and 300 °C of a magnesium matrix composite reinforced with a 10 % volume fraction of titanium particles. The Mg-10Ti (vol.% composite was processed by a powder metallurgy involving mixing of powders, cold pressing and extrusion at 400 °C. The composite shows high strength (218 MPa and some elongation (4,7 % at room temperature, higher than those reported for most of magnesium matrix composites. The gradual decrease in the mechanical properties of the composite at temperatures above 100 °C indicates the beneficial effect of titanium reinforcement.

    El presente estudio trata sobre la caracterización mecánica, en el intervalo de temperaturas comprendido entre 25 y 300 °C, del material compuesto de matriz magnesio Mg-10Ti (% vol. obtenido a través de una ruta pulvimetalúrgica que incluye la mezcla de los polvos de magnesio y titanio, compactación en frío y extrusión a 400 °C. El material muestra una elevada resistencia (218 MPa y alargamiento (4,7 % a temperatura ambiente, valores superiores a los de la mayoría de materiales compuestos de matriz magnesio reforzados con un material cerámico. La reducción en las propiedades mecánicas del material se produce de un modo gradual a partir de 100 °C, lo que indica el efecto beneficioso del refuerzo de titanio.

  18. 11B NMR Study of Magnetism in RRh3B2 (R = La, Ce, Nd, Sm, Eu and Gd)

    Science.gov (United States)

    Kishimoto, Yutaka; Kawasaki, Yu; Ohno, Takashi

    2004-07-01

    In order to study the magnetism in RRh3B2 (R = La, Ce, Nd, Sm, Eu and Gd), 11B NMR has been measured systematically. LaRh3B2 was shown to be simply paramagnetic in the normal state, and GdRh3B2 and NdRh3B2 ferromagnetic with the well localized moments. In CeRh3B2, the K-χ plot and 1/T1 can be interpreted in a localized moment model with a large reduction in the effective moment, and a small magnetic moment is considered to be localized at the Ce site below Tc. For SmRh3B2, 1/T1 cannot be explained by the localized moment model, and it was found that the antiferromagnetic spin fluctuation exists in the paramagnetic state. The temperature dependence of 1/T1T indicates that EuRh3B2 is a weak antiferromagnet.

  19. Well data summary sheets. Vol. 18. Danish North Sea

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-03-01

    This eighteenth volume of Well Data Summary Sheets includes data from five North Sea wells: Jeppe-1, Ravn-2, Gert-3, Elly-2, N-22/22A (Deep Gorm). Minor corrections have been made since the first edition. The `Complete index of released wells` has been updated. Volume 27, 28 and 29 are regional volumes; volume 27 includes well data from Southern Jutland; volume 28 and 29 cover well data from Northern Jutland. All data from released Danish North Sea wells are included in volumes 17-26 and 30. Data references are made to the revised volumes and not to the original published reference. (au)

  20. Development of a graphic interface for the Ramona-3B code; Desarrollo de una interfaz grafica para el codigo Ramona-3B

    Energy Technology Data Exchange (ETDEWEB)

    Maldonado D, D.; Santos O, M.A. [Laboratorio de Analisis de Ingenieria de Reactores Nucleares, Paseo Cuauhnahuac 8532, Jiutepec, Morelos (Mexico)]. e-mail: chalismx@hotmail.com

    2003-07-01

    In this work a graphic interface that interprets the data of the Ramona-3B code is presented. The Ramona-3B code it is a computer program, that it uses text files as input and its generate output also of this type. The quantity of generated information is so big that always it is necessary to process this information with graphic tools to be able to analyze the results of the simulations of nuclear centrals with boiling water reactors. When having a modern tool that it translates text in graphics in an automatic way and that it is of great versatility, one can obtain a graphic interface that facilitates the interpretation of how a BWR nuclear plant behaves. To achieve this tool the key it has been a program that it reads chains of previously indicated characters that keeps the data in a file for later to manipulate them in the creation of the graphic interface. It is used a software of easy access that resists the processing of a great one quantity of data and that later its have been able to graph. Another important function of this interface it is allowing the modification of the input file for Ramona using graphic unfolding and helps in it lines without necessarily to go to the file with input data. For the design of graphic interface it was decided first to show the more representative variables of a BWR type nuclear plant. It is used Mat lab as platform on several options, as PHP, Lab view or C{sup '}. The obtained graphs allow monitoring the plant and to have the control of selected variables. For the graphic interface only is necessary to indicate it the variable to simulate for to be able to interpret graphically the behavior of the BWR type nuclear plant. This tool is of great utility for the teaching of students that they are interested in this type of nuclear topics. (Author)

  1. Antibody-mediated complement C3b/iC3b binding to group B Streptococcus in paired mother and baby serum samples in a refugee population on the Thailand-Myanmar border.

    Science.gov (United States)

    Herbert, Jenny; Thomas, Stephen; Brookes, Charlotte; Turner, Claudia; Turner, Paul; Nosten, Francois; Le Doare, Kirsty; Hudson, Michael; Heath, Paul T; Gorringe, Andrew; Taylor, Stephen

    2015-03-01

    Streptococcus agalactiae (group B streptococcus [GBS]) is the leading cause of neonatal sepsis and meningitis. In this study, we determined antibody-mediated deposition of complement C3b/iC3b onto the bacterial cell surface of GBS serotypes Ia, Ib, II, III, and V. This was determined for 520 mother and umbilical cord serum sample pairs obtained at the time of birth from a population on the Thailand-Myanmar border. Antibody-mediated deposition of complement C3b/iC3b was detected to at least one serotype in 91% of mothers, despite a known carriage rate in this population of only 12%. Antibody-mediated C3b/iC3b deposition corresponded to known carriage rates, with the highest levels of complement deposition observed onto the most prevalent serotype (serotype II) followed by serotypes Ia, III, V, and Ib. Finally, neonates born to mothers carrying serotype II GBS at the time of birth showed higher antibody-mediated C3b/iC3b deposition against serotype II GBS than neonates born to mothers with no serotype II carriage. Assessment of antibody-mediated C3b/iC3b deposition against GBS may provide insights into the seroepidemiology of anti-GBS antibodies in mothers and infants in different populations.

  2. Radiation effects and tritium technology for fusion reactors. Volume I. Proceedings of the international conference, Gatlinburg, Tennessee, October 1--3, 1975

    Energy Technology Data Exchange (ETDEWEB)

    Watson, J.S.; Wiffen, F.W.; Bishop, J.L.; Breeden, B.K. (eds.)

    1976-03-01

    Separate abstracts were prepared for the 29 included papers in Vol. I. The topics covered in this volume include swelling and microstructures in thermonuclear reactor materials. Some papers on modeling and damage analysis are included. (MOW)

  3. The new silver borate Ag{sub 3}B{sub 5}O{sub 9}

    Energy Technology Data Exchange (ETDEWEB)

    Sohr, Gerhard; Falkowski, Viktoria; Huppertz, Hubert, E-mail: hubert.huppertz@uibk.ac.at

    2015-05-15

    Single crystals of Ag{sub 3}B{sub 5}O{sub 9} were obtained via high-pressure synthesis at 3 GPa and 600 °C, using a Walker-type multianvil high-pressure device. Ag{sub 3}B{sub 5}O{sub 9} crystalizes with a=674.7(2), b=943.5(2), c=1103.5(2) pm, V=0.7025(2) nm{sup 3}, and Z=4 in the noncentrosymmetric space group P2{sub 1}2{sub 1}2{sub 1} (no. 19). The orthorhombic structure was refined from 3740 independent reflections with R1=0.0496 and wR2=0.587 (all data). It is built up from infinite corner-sharing chains of BO{sub 4} tetrahedra along the a axis, which are interconnected by BO{sub 3} groups to form a network. In the structure, three crystallographically independent sites are occupied with Ag{sup +} cations exhibiting argentophillic interactions. The synthetic conditions as well as the results of the single crystal structure analysis are presented. - Graphical abstract: Noncentrosymmetric silver borate: During investigations in the system Ag–B–O, a new noncentrosymmetric silver borate Ag{sub 3}B{sub 5}O{sub 9} was discovered. The new structure type is built up from corner-sharing BO{sub 3} and BO{sub 4} groups, forming a network. Argentophillic interactions are clearly indicated by the Ag{sup +}⋯Ag{sup +} distances present in the structure. - Highlights: • A noncentrosymmetric borate Ag{sub 3}B{sub 5}O{sub 9} is accessible via high-pressure synthesis. • Ag{sub 3}B{sub 5}O{sub 9} is the second high-pressure silver borate. • Ag{sup +}⋯Ag{sup +} distances in Ag3B5O9 clearly indicate the presence of argentophillic interactions.

  4. Repetitively Pulsed Electric Laser Acoustic Studies. Volume 1.

    Science.gov (United States)

    1983-09-01

    INGARD ET AL. SEP 83 UNCLASSIFIED APHAL-IR-83-2858-VOL-1 F336i5 86-C 2848 F/ 0/ 8, EEEmohEEEomhiE EohEEmhohEEEEE mhhhmmomhhlm...TR-83-2058, Vol 9, 0 REPETITIVELY PULSED ELECTRIC LASER ACOUSTIC STUDIES Volume I K. U. INGARD , CHARLES F. MCMILLAN uDEPARTMENT OF AERONAUTICS AND...CONTRACT OR GRANT NUMBER(s) K.U. Ingard and Charles F. McMillan F33615.80-C-2040 9. PERFORMING ORGANIZATION NAME AND ADDRESS 10. PROGRAM ELEMENT. PROJECT

  5. Volume Entropy

    CERN Document Server

    Astuti, Valerio; Rovelli, Carlo

    2016-01-01

    Building on a technical result by Brunnemann and Rideout on the spectrum of the Volume operator in Loop Quantum Gravity, we show that the dimension of the space of the quadrivalent states --with finite-volume individual nodes-- describing a region with total volume smaller than $V$, has \\emph{finite} dimension, bounded by $V \\log V$. This allows us to introduce the notion of "volume entropy": the von Neumann entropy associated to the measurement of volume.

  6. Oak Ridge Reservation environmental report for 1992. Volume 1: Narrative

    Energy Technology Data Exchange (ETDEWEB)

    Koncinski, W.S. [ed.

    1993-09-01

    The two volumes of this report present data and supporting narratives regarding the impact of the US Department of Energy`s (DOE`s) Oak Ridge Reservation (ORR) on its environs and the public during 1992. This Volume (Volume 1) includes all narrative descriptions, summaries, and conclusions and is intended to be a ``stand-alone`` report for the reader who does not want to review in detail all of the 1992 data for the ORR. Volume 2 includes the detailed data in formats that ensure all the environmental data are represented. Narratives are not included in Vol. 2.

  7. Effects of Lu-Do-Huang Extract (LDHE) on Apoptosis Induction in Human Hep3B Cells.

    Science.gov (United States)

    Huang, Hui-Yu; Chen, Li-Han; Liu, Chen-Wei; Chien, Ting-Yi; Yu, Yu-Ping; Kao, Yu-Yu; Yang, Jo-Hsuan; Tsai, Ying-Chieh

    2015-06-30

    Lu-Do-Huang (Pracparatum mungo) is a fermented mung bean [corrected] (Vigna radiata) and has long been used as a traditional and functional food in Traditional Chinese Medicine, especially for treating a variety of liver disorders. The present study aimed to evaluate the apoptotic effects of Lu-Do-Huang ethanol extract (LDHE) on Hep3B cells, a human hepatoma cell line. A variety of cellular assays, flow cytometry and immunoblotting were used. Our results showed that LDHE significantly inhibited Hep3B cells growth. Additionally, the cell cycle assay showed that LDHE prevented Hep3B cell entry into S phase and led to an arrest of Hep3B cells in the G₀/G₁ phase. LDHE induced Hep3B cells to undergo apoptosis as determined through Hep3B cell morphology changes, increase of apoptotic bodies, apoptotic cells, DNA fragmentations and caspase activity. We further examined the protein expression of TRADD, FADD, and Bax to verify the possible apoptotic pathways. The results indicated that LDHE-induced apoptosis in Hep3B cells might be mediated [corrected] by an extrinsic signaling pathway leading to an induction of apoptosis in Hep3B cells. In conclusion, LDHE induced apoptosis and cell cycle arrest in Hep3B cells. Our data provide the evidences regarding the anti-hepatoma potential of LDHE in Hep3B cells.

  8. A single amino acid substitution confers enhanced methylation activity of mammalian Dnmt3b on chromatin DNA.

    Science.gov (United States)

    Shen, Li; Gao, Ge; Zhang, Ying; Zhang, He; Ye, Zhiqiang; Huang, Shichao; Huang, Jinyan; Kang, Jiuhong

    2010-10-01

    Dnmt3a and Dnmt3b are paralogous enzymes responsible for de novo DNA methylation but with distinguished biological functions. In mice, disruption of Dnmt3b but not Dnmt3a causes global DNA hypomethylation, especially in repetitive sequences, which comprise the large majority of methylated DNA in the genome. By measuring DNA methylation activity of Dnmt3a and Dnmt3b homologues from five species, we found that mammalian Dnmt3b possessed significantly higher methylation activity on chromatin DNA than Dnmt3a and non-mammalian Dnmt3b. Sequence comparison and mutagenesis experiments identified a single amino acid substitution (I662N) in mammalian Dnmt3b as being crucial for its high chromatin DNA methylation activity. Further mechanistic studies demonstrated this substitution markedly enhanced the binding of Dnmt3b to nucleosomes and hence increased the chromatin DNA methylation activity. Moreover, this substitution was crucial for Dnmt3b to efficiently methylate repetitive sequences, which increased dramatically in mammalian genomes. Consistent with our observation that Dnmt3b evolved more rapidly than Dnmt3a during the emergence of mammals, these results demonstrated that the I662N substitution in mammalian Dnmt3b conferred enhanced chromatin DNA methylation activity and contributed to functional adaptation in the epigenetic system.

  9. The RNA-binding protein HuR regulates DNA methylation through stabilization of DNMT3b mRNA.

    Science.gov (United States)

    López de Silanes, Isabel; Gorospe, Myriam; Taniguchi, Hiroaki; Abdelmohsen, Kotb; Srikantan, Subramanya; Alaminos, Miguel; Berdasco, María; Urdinguio, Rocío G; Fraga, Mario F; Jacinto, Filipe V; Esteller, Manel

    2009-05-01

    The molecular basis underlying the aberrant DNA-methylation patterns in human cancer is largely unknown. Altered DNA methyltransferase (DNMT) activity is believed to contribute, as DNMT expression levels increase during tumorigenesis. Here, we present evidence that the expression of DNMT3b is post-transcriptionally regulated by HuR, an RNA-binding protein that stabilizes and/or modulates the translation of target mRNAs. The presence of a putative HuR-recognition motif in the DNMT3b 3'UTR prompted studies to investigate if this transcript associated with HuR. The interaction between HuR and DNMT3b mRNA was studied by immunoprecipitation of endogenous HuR ribonucleoprotein complexes followed by RT-qPCR detection of DNMT3b mRNA, and by in vitro pulldown of biotinylated DNMT3b RNAs followed by western blotting detection of HuR. These studies revealed that binding of HuR stabilized the DNMT3b mRNA and increased DNMT3b expression. Unexpectedly, cisplatin treatment triggered the dissociation of the [HuR-DNMT3b mRNA] complex, in turn promoting DNMT3b mRNA decay, decreasing DNMT3b abundance, and lowering the methylation of repeated sequences and global DNA methylation. In summary, our data identify DNMT3b mRNA as a novel HuR target, present evidence that HuR affects DNMT3b expression levels post-transcriptionally, and reveal the functional consequences of the HuR-regulated DNMT3b upon DNA methylation patterns.

  10. Sorption of wastewater containing reactive red X-3B on inorgano-organo pillared bentonite

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Bentonite is a kind of natural clay with good exchanging ability. By exchanging its interlamellar cations with various soluble cations, such as quaternary ammonium cations and inorganic metal ions, the properties of natural bentonite can be greatly improved. In this study, hexadecyltrimethylammonium bromide (HDTMA), CaCl2, MgCl2, FeCl3, AlCl3 were used as organic and inorganic pillared materials respectively to produce several kinds of Ca-, Mg-, Fe-, Al-organo pillared bentonites. Sorption of reactive red X-3B on them was studied to determine their potential application as sorbents in wastewater treatment. The results showed that these pillared bentonites had much improved sorption properties, and that the dye solutions' pH value had some effect on the performance of these inorgano-organo pillared bentonites. Isotherms of reactive X-3B on these pillared bentonites suggested a Langmuir-type sorption mechanism.

  11. Evaluation of T3B fingerprinting for identification of clinical and environmental Sporothrix species.

    Science.gov (United States)

    Oliveira, Manoel Marques Evangelista; Franco-Duarte, Ricardo; Romeo, Orazio; Pais, Célia; Criseo, Giuseppe; Sampaio, Paula; Zancope-Oliveira, Rosely Maria

    2015-03-01

    In this study, PCR fingerprinting using the universal primer T3B was applied to distinguish among clinical and environmental species of the Sporothrix complex, Sporothrix brasiliensis, S. globosa, S. mexicana, S. pallida, S. luriei and S. schenckii sensu stricto. The T3B fingerprinting generated clearly distinct banding patterns, allowing the correct identification of all 43 clinical and environmental isolates at the species level, what was confirmed by partial calmodulin gene sequence analyses. This technique is reproducible and provides the identification of all species of the Sporothrix complex with sufficient accuracy to be applied in clinical mycology laboratories as well as in epidemiological studies in order to obtain a better understanding of the epidemiology of sporotrichosis.

  12. Low-energy magnetic excitation spectrum of the unconventional ferromagnet CeRh3B2

    Science.gov (United States)

    Raymond, S.; Panarin, J.; Givord, F.; Murani, A. P.; Boucherle, J. X.; Lejay, P.

    2010-09-01

    The magnetic excitation spectrum of the unconventional ferromagnet CeRh3B2 was measured by inelastic neutron scattering on single-crystal sample in the magnetically ordered and paramagnetic phases. The spin-wave excitation spectrum evidences high exchange interaction along the c axis about two orders of magnitude higher than the ones in the basal plane of the hexagonal structure. Both strong out-of-plane and small in-plane anisotropies are found. This latter point confirms that considering the J=5/2 multiplet alone is not adequate for describing the ground state of CeRh3B2 . Quasielastic scattering measured above TCurie is also strongly anisotropic between the basal plane and the c axis and suggests localized magnetism.

  13. Batch conversion of methane to methanol using Methylosinus trichosporium OB3b as biocatalyst.

    Science.gov (United States)

    Hwang, In Yeub; Hur, Dong Hoon; Lee, Jae Hoon; Park, Chang-Ho; Chang, In Seop; Lee, Jin Won; Lee, Eun Yeol

    2015-03-01

    Recently, methane has attracted much attention as an alternative carbon feedstock since it is the major component of abundant shale and natural gas. In this work, we produced methanol from methane using whole cells of Methylosinus trichosporium OB3b as the biocatalyst. M. trichosporium OB3b was cultured on NMS medium with a supply of 7:3 air/methane ratio at 30°C. The optimal concentrations of various methanol dehydrogenase inhibitors such as potassium phosphate and EDTA were determined to be 100 and 0.5 mM, respectively, for an efficient production of methanol. Sodium formate (40 mM) as a reducing power source was added to enhance the conversion efficiency. A productivity of 49.0 mg/l·h, titer of 0.393 g methanol/l, and conversion of 73.8% (mol methanol/mol methane) were obtained under the optimized batch condition.

  14. Magnetic Properties of Nd8Fe83Co3B6 Nanocomposite Magnets

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The influence of quenching technology, annealing temperature and time on the structures and magnetic properties of Nd8Fe83Co3B6 nanocomposite magnets was investigated. The results show that the α-Fe/Nd2Fe14B nanocomposite magnet containing a small amount of B is difficult to form amorphous state. The magnetic properties of 26 m/s quenched Nd8Fe83Co3B6 powders annealed at 640℃×480 s reach iHc=513 kA/m, Br=1.05 T and (BH)max=92.0 kJ/m3. The grain size is Dα-Fe=21.5 nm and DNd2Fe14B=30.2 nm.

  15. Quantitative morphometry of electrophysiologically identified CA3b interneurons reveals robust local geometry and distinct cell classes.

    Science.gov (United States)

    Ascoli, Giorgio A; Brown, Kerry M; Calixto, Eduardo; Card, J Patrick; Galván, E J; Perez-Rosello, T; Barrionuevo, Germán

    2009-08-20

    The morphological and electrophysiological diversity of inhibitory cells in hippocampal area CA3 may underlie specific computational roles and is not yet fully elucidated. In particular, interneurons with somata in strata radiatum (R) and lacunosum-moleculare (L-M) receive converging stimulation from the dentate gyrus and entorhinal cortex as well as within CA3. Although these cells express different forms of synaptic plasticity, their axonal trees and connectivity are still largely unknown. We investigated the branching and spatial patterns, plus the membrane and synaptic properties, of rat CA3b R and L-M interneurons digitally reconstructed after intracellular labeling. We found considerable variability within but no difference between the two layers, and no correlation between morphological and biophysical properties. Nevertheless, two cell types were identified based on the number of dendritic bifurcations, with significantly different anatomical and electrophysiological features. Axons generally branched an order of magnitude more than dendrites. However, interneurons on both sides of the R/L-M boundary revealed surprisingly modular axodendritic arborizations with consistently uniform local branch geometry. Both axons and dendrites followed a lamellar organization, and axons displayed a spatial preference toward the fissure. Moreover, only a small fraction of the axonal arbor extended to the outer portion of the invaded volume, and tended to return toward the proximal region. In contrast, dendritic trees demonstrated more limited but isotropic volume occupancy. These results suggest a role of predominantly local feedforward and lateral inhibitory control for both R and L-M interneurons. Such a role may be essential to balance the extensive recurrent excitation of area CA3 underlying hippocampal autoassociative memory function.

  16. Mastoplastia reductora en L para grandes volúmenes

    OpenAIRE

    Fernandez, Claudio de Barros

    2013-01-01

    Las primeras descripciones de las mastoplastias fueron atribuidas a Paulus Aegineta en 625-690d.C. 4, con una resección de ginecomastia por el surco sub-mamario. Posteriormente, muchas técnicas fueron descritas para la disminución del órgano. Clásicamente, fueron consideradas mamas grandes aquellas con volúmenes resecados más grandes que 600 gr., siendo la mayoría tratadas tras cicatrices en T invertido. El objetivo de ese estudio es mostrar que es posible tratar mamas grandes con cicatrices ...

  17. Mastoplastia reductora en l para grandes volúmenes

    OpenAIRE

    De Barros Fernandez, Claudio

    2012-01-01

    Las primeras descripciones de las mastoplastias fueron atribuidas a Paulus Aegineta en 625-690d.C. 4, con una resección de ginecomastia por el surco sub-mamario. Posteriormente, muchas técnicas fueron descritas para la disminución del órgano. Clásicamente, fueron consideradas mamas grandes aquellas con volúmenes resecados más grandes que 600 gr., siendo la mayoría tratadas tras cicatrices en T invertido. El objetivo de ese estudio es mostrar que es posible tratar mamas grandes ...

  18. Sensitometria : sistema analògic versus digital. Vol. 2

    OpenAIRE

    Llunas i Pérez, Pere; Mur i Sanabre, Carme de

    2004-01-01

    Aquesta publicació vol ser una ajuda per a totes aquelles persones interessades en el coneixement dels materials fotogràfics fotoquímics i digitals i en el seu processament químic i digital. Al mateix temps pretén que alguns apartats d'aquesta disciplina siguin més propers a la pràctica fotogràfica. L'obra es composa d'il·lustracions que han estat dissenyades amb una finalitat docent; aquestes il·lustracions s'utilitzen a classe per a desenvolupar els temes que configuren el programa de l'ass...

  19. Componentes volátiles de mamey (mammea americana L.)

    OpenAIRE

    Alicia Lucía Morales; William Arguello; Gustavo García; Iván Herrera

    2010-01-01

    Los componentes volátiles del aroma de mamey (Mammea americana L), fueron extraídos utilizando el método de destilación por arrastre con vapor-extracción simultánea con solvente orgánico. El extracto fue prefraccionado por cromatografía en columna en silica gel con gradiente discontinuo Pentano: Éter etílico para obtener tres fracciones que fueron analizadas por CGAR y CGAR-EM. Se detectaron 34 compuestos, de los cuales fueron identificados 22, siendo los componentes mayoritarios: Furfural (7...

  20. Componentes volátiles de mamey (mammea americana l.)

    OpenAIRE

    Morales, Alicia Lucía; Arguello, William; García, Gustavo; Herrera, Iván

    2010-01-01

    Los componentes volátiles del aroma de mamey (Mammea americana L), fueron extraídos utilizando el método de destilación por arrastre con vapor-extracción simultánea con solvente orgánico. El extracto fue prefraccionado por cromatografía en columna en silica gel con gradiente discontinuo Pentano: Éter etílico para obtener tres fracciones que fueron analizadas por CGAR y CGAR-EM. Se detectaron 34 compuestos, de los cuales fueron identificados 22, siendo los componentes mayoritarios: Furfural (7...

  1. Diagramas de flujo y volúmenes de control

    OpenAIRE

    Fernández Torres, María José

    2010-01-01

    En este vídeo se explican los conceptos de DIAGRAMA DE FLUJO y de VOLÚMEN DE CONTROL aplicados a la Ingeniería Química. Se detalla cómo se dibujan correctamente volúmenes de control y las corrientes que deben considerarse en varios casos. Participan: Voz: Dra. María José Fernández Torres; Colaboran: Dra. María Dolores Saquete Ferrándiz y Dr. Francisco Ruiz Beviá.

  2. Quadrupole interaction at111Cd in RRh3B2 compounds (R=Ce to Gd) and absence of transferred hyperfine field at111Cd in GdRh3B2

    Science.gov (United States)

    Devare, S. H.; Dhar, S. K.; Malik, S. K.; Devare, H. G.

    1983-12-01

    The quadrupole interaction at111Cd in RRh3B2 compounds studied by the TDPAC technique supports the prediction that Ce in CeRh3B2 is not trivalent. The highly anisotropic EFG indicates that111Cd in GdRh3B2 cannot occupy the rare earth site. The transferred magnetic hyperfine field at111Cd is nearly zero which supports the conjecture that the impurity probe nuclei are at the 3g(Rh) site.

  3. A LM-3B Launch Vehicle Sent Chinasat-9 Into Space

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ The Chinasat-9 direct-broadcast television satellite was launched by a LM-3B launch vehicle from Xichang Satellite Launch Center (XSLC) on June 9.According to Xi'an Satellite Control Center (XSCC),the satellite entered the preset super-geosynchronous transfer orbit 26 minutes after the liftoff with an apogee of 49887km,a perigee of 214km and an inclination of 24.2 degrees.

  4. 17 CFR 240.3b-6 - Liability for certain statements by issuers.

    Science.gov (United States)

    2010-04-01

    ... (§ 240.14a-3(b) and (c) or § 240.14c-3(a) and (b) of this chapter) and that relates to: (i) The effects... Regulation S-K (§ 229.303 of this chapter), “Management's Discussion and Analysis of Financial Condition and... limited to: (1) A statement containing a projection of revenues, income (loss), earnings (loss) per...

  5. Synthesis and antiplasmodial evaluation of novel chromeno[2,3-b]chromene derivatives.

    Science.gov (United States)

    Devakaram, Ruth; Black, David StC; Choomuenwai, Vanida; Davis, Rohan A; Kumar, Naresh

    2012-02-15

    5-Methoxyflavenes and 6-methoxyflavenes were found to undergo stereoselective acid-catalyzed rearrangement to generate a range of novel chromeno[2,3-b]chromene derivatives. When subjected to an in vitro antiplasmodial growth inhibition assay using Plasmodium falciparum (3D7 line) the chromene analogues were shown to display IC(50) values ranging from 6.8 to 39.8 μM. Copyright © 2011 Elsevier Ltd. All rights reserved.

  6. DNA Methyltransferase 3B Gene Promoter and Interleukin-1 Receptor Antagonist Polymorphisms in Childhood Immune Thrombocytopenia

    Directory of Open Access Journals (Sweden)

    Margarita Pesmatzoglou

    2012-01-01

    Full Text Available Primary immune thrombocytopenia (ITP is one of the most common blood diseases as well as the commonest acquired bleeding disorder in childhood. Although the etiology of ITP is unclear, in the pathogenesis of the disease, both environmental and genetic factors including polymorphisms of TNF-a, IL-10, and IL-4 genes have been suggested to be involved. In this study, we investigated the rs2424913 single-nucleotide polymorphism (SNP (C46359T in DNA methyltransferase 3B (DNMT3B gene promoter and the VNTR polymorphism of IL-1 receptor antagonist (IL-1 Ra intron-2 in 32 children (17 boys with the diagnosis of ITP and 64 healthy individuals. No significant differences were found in the genotype distribution of DNMT3B polymorphism between the children with ITP and the control group, whereas the frequency of allele T appeared significantly increased in children with ITP (P = 0.03, OR = 2, 95% CI: 1.06–3.94. In case of IL-1 Ra polymorphism, children with ITP had a significantly higher frequency of genotype I/II, compared to control group (P = 0.043, OR = 2.60, 95% CI: 1.02–6.50. Moreover, genotype I/I as well as allele I was overrepresented in the control group, suggesting that allele I may have a decreased risk for development of ITP. Our findings suggest that rs2424913 DNMT3B SNP as well as IL-1 Ra VNTR polymorphism may contribute to the susceptibility to ITP.

  7. Low energy magnetic excitation spectrum of the unconventional ferromagnet CeRh$_{3}$B$_{2}$

    OpenAIRE

    2010-01-01

    The magnetic excitation spectrum of the unconventional ferromagnet CeRh$_{3}$B$_{2}$ was measured by inelastic neutron scattering on single crystal sample in the magnetically ordered and paramagnetic phases. The spin-wave excitation spectrum evidences high exchange interaction along the c-axis about two orders of magnitude higher than the ones in the basal plane of the hexagonal structure. Both strong out of plane and small in plane anisotropies are found. This latter point confirms that cons...

  8. Fermi surfaces of CeRh3B2: An LSDA+ U study

    Science.gov (United States)

    Yamauchi, K.; Yanase, A.; Harima, H.

    2006-05-01

    The Fermi surfaces for ferromagnetic CeRh3B2 are calculated by using FLAPW and LSDA+ U method. The result reveals that the nine Fermi surfaces show the large spin split due to the magnetic contribution of the occupied 4f1 electron. The occupied Ce-4f component which shows large dispersion due to the extremely short c-distance of the crystal structure might be related to the high Curie temperature.

  9. On the unusual magnetic behaviour of CeRh3B2

    Science.gov (United States)

    Galatanu, A.; Yamamoto, E.; Okubo, T.; Yamada, M.; Thamizhavel, A.; Takeuchi, T.; Sugiyama, K.; Inada, Y.; Onuki, Y.

    2003-07-01

    CeRh3B2 is a ferromagnet with an unexpectedly high Curie temperature and low saturation magnetic moments. The origin of its unusual magnetic behaviour remains unelucidated despite various investigations performed in the last two decades. Here we present magnetic results obtained on high-quality single crystals over a wide temperature range. Some new magnetic features are uncovered and possible approaches to explaining its magnetism are briefly discussed.

  10. De Novo DNA Methyltransferase DNMT3b Interacts with NEDD8-modified Proteins*

    OpenAIRE

    Shamay, Meir; Greenway, Melanie; Liao, Gangling; AMBINDER, RICHARD F; Hayward, S. Diane

    2010-01-01

    DNA methylation and histone modifications play an important role in transcription regulation. In cancer cells, many promoters become aberrantly methylated through the activity of the de novo DNA methyltransferases DNMT3a and DNMT3b and acquire repressive chromatin marks. NEDD8 is a ubiquitin-like protein modifier that is conjugated to target proteins, such as cullins, to regulate their activity, and cullin 4A (CUL4A) in its NEDD8-modified form is essential for repressive chromatin formation. ...

  11. Qatar Exoplanet Survey : Qatar-3b, Qatar-4b and Qatar-5b

    CERN Document Server

    Alsubai, Khalid A; Tsvetanov, Zlatan I; Latham, David W; Bieryla, Allyson; Buchhave, Lars A; Esquerdo, Gilbert A; Bramich, D M; Pyrzas, Stylianos; Vilchez, Nicolas P E; Mancini, Luigi; Southworth, John; Evans, Daniel F; Henning, Thomas; Ciceri, Simona

    2016-01-01

    We report the discovery of Qatar-3b, Qatar-4b, and Qatar-5b, three new transiting planets identified by the Qatar Exoplanet Survey (QES). The three planets belong to the hot Jupiter family, with orbital periods of $P_{Q3b}$=2.5079204 days, $P_{Q4b}$=1.8053949 days, and $P_{Q5b}$=2.8792319 days. Follow-up spectroscopic observations reveal the masses of the planets to be $M_{Q3b}$=4.31$M_{\\rm J}$, $M_{Q4b}$=5.85$M_{\\rm J}$, and $M_{Q5b}$=4.32$M_{\\rm J}$, while model fits to the transit light curves yield radii of $R_{Q3b}$=1.096$R_{\\rm J}$, $R_{Q4b}$=1.552$R_{\\rm J}$, and $R_{Q5b}$=1.107$R_{\\rm J}$. No evidence of eccentric orbit is seen in the radial velocity curve of any of the planets. The host stars are typical main sequence stars with masses and radii $M_{Q3}$=1.145$M_{\\odot}$, $M_{Q4}$=0.954$M_{\\odot}$, $M_{Q5}$=1.128$M_{\\odot}$ and $R_{Q3}$=1.272$R_{\\odot}$, $R_{Q4}$=1.115$R_{\\odot}$ and $R_{Q5}$=1.076$R_{\\odot}$ for the Qatar-3, 4 and 5 respectively. All three new planets can be classified as heavy hot ...

  12. CT-measured regional specific volume change reflects regional ventilation in supine sheep.

    Science.gov (United States)

    Fuld, Matthew K; Easley, R Blaine; Saba, Osama I; Chon, Deokiee; Reinhardt, Joseph M; Hoffman, Eric A; Simon, Brett A

    2008-04-01

    Computer tomography (CT) imaging techniques permit the noninvasive measurement of regional lung function. Regional specific volume change (sVol), determined from the change in lung density over a tidal breath, should correlate with regional ventilation and regional lung expansion measured with other techniques. sVol was validated against xenon (Xe)-CT-specific ventilation (sV) in four anesthetized, intubated, mechanically ventilated sheep. Xe-CT used expiratory gated axial scanning during the washin and washout of 55% Xe. sVol was measured from the tidal changes in tissue density (H, houndsfield units) of lung regions using the relationship sVol = [1,000(Hi - He)]/[He(1,000 + Hi)], where He and Hi are expiratory and inspiratory regional density. Distinct anatomical markings were used to define corresponding lung regions of interest between inspiratory, expiratory, and Xe-CT images, with an average region of interest size of 1.6 +/- 0.7 ml. In addition, sVol was compared with regional volume changes measured directly from the positions of implanted metal markers in an additional animal. A linear relationship between sVol and sV was demonstrated over a wide range of regional sV found in the normal supine lung, with an overall correlation coefficient (R(2)) of 0.66. There was a tight correlation (R(2) = 0.97) between marker-measured volume changes and sVol. Regional sVol, which involves significantly reduced exposure to radiation and Xe gas compared with the Xe-CT method, represents a safe and efficient surrogate for measuring regional ventilation in experimental studies and patients.

  13. Oak Ridge National Laboratory Technology Logic Diagram. Volume 2, Technology Logic Diagram: Part B, Remedial Action

    Energy Technology Data Exchange (ETDEWEB)

    1993-09-01

    The Oak Ridge National Laboratory Technology Logic Diagram (TLD) was developed to provide a decision support tool that relates environmental restoration (ER) and waste management (WM) problems at Oak Ridge National Laboratory (ORNL) to potential technologies that can remediate these problems. The TLD identifies the research, development, demonstration, testing, and evaluation needed to develop these technologies to a state that allows technology transfer and application to decontamination and decommissioning (D&D), remedial action (RA), and WM activities. The TLD consists of three fundamentally separate volumes: Vol. 1 (Technology Evaluation), Vol. 2 (Technology Logic Diagram), and Vol. 3 (Technology Evaluation Data Sheets). Part A of Vols. 1. and 2 focuses on D&D. Part B of Vols. 1 and 2 focuses on the RA of contaminated facilities. Part C of Vols. 1 and 2 focuses on WM. Each part of Vol. 1 contains an overview of the TLD, an explanation of the program-specific responsibilities, a review of identified technologies, and the rankings of remedial technologies. Volume 2 (Pts. A, B, and C) contains the logic linkages among EM goals, environmental problems, and the various technologies that have the potential to solve these problems. Volume 3 (Pts. A, B, and C) contains the TLD data sheets. Remedial action is the focus of Vol. 2, Pt. B, which has been divided into the three necessary subelements of the RA: characterization, RA, and robotics and automation. Each of these sections address general ORNL problems, which are then broken down by problem area/constituents and linked to potential remedial technologies. The diagrams also contain summary information about a technology`s status, its science and technology needs, and its implementation needs.

  14. Novel 1H-pyrrolo[2,3-b]pyridine derivative nortopsentin analogues: synthesis and antitumor activity in peritoneal mesothelioma experimental models.

    Science.gov (United States)

    Carbone, Anna; Pennati, Marzia; Parrino, Barbara; Lopergolo, Alessia; Barraja, Paola; Montalbano, Alessandra; Spanò, Virginia; Sbarra, Stefania; Doldi, Valentina; De Cesare, Michelandrea; Cirrincione, Girolamo; Diana, Patrizia; Zaffaroni, Nadia

    2013-09-12

    In this study, we describe the synthesis of new nortopsentin analogues, 1H-pyrrolo[2,3-b]pyridine derivatives and their biological effects in experimental models of diffuse malignant peritoneal mesothelioma (DMPM), a rare and rapidly fatal disease, poorly responsive to conventional therapies. The three most active compounds, 1f (3-[2-(5-fluoro-1-methyl-1H-indol-3-yl)-1,3-thiazol-4-yl]-1H-pyrrolo[2,3-b]pyridine), 3f (3-[2-(1H-indol-3-yl)-1,3-thiazol-4-yl]-1-methyl-1H-pyrrolo[2,3-b]pyridine), and 1l (3-[2-(5-fluoro-1-methyl-1H-indol-3-yl)-1,3-thiazol-4-yl]-1-methyl-1H-pyrrolo[2,3-b] pyridine), which were shown to act as cyclin-dependent kinase 1 inhibitors, consistently reduced DMPM cell proliferation and induced a caspase-dependent apoptotic response, with a concomitant reduction of the expression of the active Thr(34)-phosphorylated form of the antiapoptotic protein survivin. Moreover, the combined treatment of DMPM cells with 3f derivative and paclitaxel produced a synergistic cytotoxic effect, which was paralleled by an enhanced apoptotic response. In the mouse model, i.p. administration of 1f, 3f, and 1l derivatives was effective, resulting in a significant tumor volume inhibition of DMPM xenografts (range, 58-75%) at well-tolerated doses, and two complete responses were observed in each treatment group.

  15. Near-UV and optical observations of the transiting exoplanet TrES-3b

    CERN Document Server

    Turner, Jake D; Hardegree-Ullman, Kevin K; Carleton, Timothy M; Walker-LaFollette, Amanda M; Crawford, Benjamin E; Smith, Carter-Thaxton W; McGraw, Allison M; Small, Lindsay C; Rocchetto, Marco; Cunningham, Kathryn I; Towner, Allison P M; Zellem, Robert; Robertson, Amy N; Guvenen, Blythe C; Schwarz, Kamber R; Hardegree-Ullman, Emily E; Collura, Daniel; Henz, Triana N; Lejoly, Cassandra; Richardson, Logan L; Weinand, Michael A; Taylor, Joanna M; Daugherty, Michael J; Wilson, Ashley A; Austin, Carmen L

    2012-01-01

    We observed nine primary transits of the hot Jupiter TrES-3b in several optical and near-UV photometric bands from 2009 June to 2012 April in an attempt to detect its magnetic field. Vidotto, Jardine and Helling suggest that the magnetic field of TrES-3b can be constrained if its near-UV light curve shows an early ingress compared to its optical light curve, while its egress remains unaffected. Predicted magnetic field strengths of Jupiter-like planets should range between 8 G and 30 G. Using these magnetic field values and an assumed B_star of 100 G, the Vidotto et al. method predicts a timing difference of 5-11 min. We did not detect an early ingress in our three nights of near-UV observations, despite an average cadence of 68 s and an average photometric precision of 3.7 mmag. However, we determined an upper limit of TrES-3b's magnetic field strength to range between 0.013 and 1.3 G (for a 1-100 G magnetic field strength range for the host star, TrES-3) using a timing difference of 138 s derived from the N...

  16. Inter-comparison and accuracy assessment of TRMM 3B42 products over Turkey

    Science.gov (United States)

    Amjad, Muhammad; Yilmaz, M. Tugrul

    2016-04-01

    Accurate estimation of precipitation, especially over complex topography, is impeded by many factors depending on the platform that it is acquired. Satellites have the advantage of providing spatially and temporally continuous and consistent datasets. However, utilizing satellite precipitation data in various applications requires its uncertainty estimation to be carried out robustly. In this study, accuracy of two Tropical Rainfall Measurement Mission (Version 3B42) products, TRMM 3B42 V6 and TRMM3B42 V7, are assessed for their accuracy by inter-comparing their monthly time series against ground observations obtained over 256 stations in Turkey. Errors are further analyzed for their seasonal and climate-dependent variability. Both V6 and V7 products show better performance during summers than winters. V6 product has dry bias over drier regions and V7 product has wet bias over wetter regions of the country. Moreover, rainfall measuring accuracies of both versions are much lower along coastal regions and at lower altitudes. Overall, the statistics of the monthly products confirm V7 product is an improved version compared to V6. (This study was supported by TUBITAK fund # 114Y676).

  17. WASP-3b: a strongly-irradiated transiting gas-giant planet

    CERN Document Server

    Pollacco, D; Cameron, A Collier; Loeillet, B; Stempels, H C; Bouchy, F; Gibson, N P; Hebb, L; Hébrard, G; Joshi, Y C; McDonald, I; Smalley, B; Smith, A M S; Street, R A; Udry, S; West, R G; Wilson, D M; Wheatley, P J; Aigrain, S; Benn, C R; Bruce, V A; Christian, D J; Clarkson, W I; Enoch, B; Evans, A; Fitzsimmons, A; Haswell, C A; Hellier, C; Hickey, S; Hodgkin, S T; Horne, K; Hrudkova, M; Irwin, J; Kane, S R; Keenan, F P; Lister, T A; Maxted, P; Mayor, M; Moutou, C; Norton, A J; Osborne, J P; Parley, N; Pont, F; Queloz, D; Ryans, R; Simpson, E

    2007-01-01

    We report the discovery of WASP-3b, the third transiting exoplanet to be discovered by the WASP and SOPHIE collaboration. WASP-3b transits its host star USNO-B1.0 1256-0285133 every 1.846834+-0.000002 days. Our high precision radial-velocity measurements present a variation with amplitude characteristic of a planetary-mass companion and in-phase with the light-curve. Adaptive optics imaging shows no evidence for nearby stellar companions, and line-bisector analysis excludes faint, unresolved binarity and stellar activity as the cause of the radial-velocity variations. We make a preliminary spectroscopic analysis of the host star finding it to have Teff = 6400+-100 K and log g = 4.25+-0.05 which suggests it is most likely an unevolved main sequence star of spectral type F7-8V. Our simultaneous modelling of the transit photometry and reflex motion of the host leads us to derive a mass of 1.76 +0.08 -0.14 M_J and radius 1.31 +0.07-0.14 R_J for WASP-3b. The proximity and relative temperature of the host star sugg...

  18. Thermal Emission and Tidal Heating of the Heavy and Eccentric Planet XO-3b

    CERN Document Server

    Machalek, Pavel; McCullough, Peter R; Burrows, Adam; Burke, Christopher J; Hora, Joseph L; Johns-Krull, Christopher M; Deming, Drake L

    2010-01-01

    We determined the flux ratios of the heavy and eccentric planet XO-3b to its parent star in the four IRAC bands of the Spitzer Space Telescope: 0.101% +- 0.004% at 3.6 micron; 0.143% +- 0.006% at 4.5 micron; 0.134% +- 0.049% at 5.8 micron and 0.150% +- 0.036% at 8.0 micron. The flux ratios are within [-2.2,0.3, -0.8, -1.7]-sigma of the model of XO-3b with a thermally inverted stratosphere in the 3.6 micron, 4.5 micron, 5.8 micron and 8.0 micron channels, respectively. XO-3b has a high illumination from its parent star (Fp ~(1.9 - 4.2) x 10^9 ergs cm^-2 s^-1) and is thus expected to have a thermal inversion, which we indeed observe. When combined with existing data for other planets, the correlation between the presence of an atmospheric temperature inversion and the substellar flux is insufficient to explain why some high insolation planets like TrES-3 do not have stratospheric inversions and some low insolation planets like XO-1b do have inversions. Secondary factors such as sulfur chemistry, atmospheric met...

  19. MiR-221 promotes stemness of breast cancer cells by targeting DNMT3b.

    Science.gov (United States)

    Roscigno, Giuseppina; Quintavalle, Cristina; Donnarumma, Elvira; Puoti, Ilaria; Diaz-Lagares, Angel; Iaboni, Margherita; Fiore, Danilo; Russo, Valentina; Todaro, Matilde; Romano, Giulia; Thomas, Renato; Cortino, Giuseppina; Gaggianesi, Miriam; Esteller, Manel; Croce, Carlo M; Condorelli, Gerolama

    2016-01-05

    Cancer stem cells (CSCs) are a small part of the heterogeneous tumor cell population possessing self-renewal and multilineage differentiation potential as well as a great ability to sustain tumorigenesis. The molecular pathways underlying CSC phenotype are not yet well characterized. MicroRNAs (miRs) are small noncoding RNAs that play a powerful role in biological processes. Early studies have linked miRs to the control of self-renewal and differentiation in normal and cancer stem cells. We aimed to study the functional role of miRs in human breast cancer stem cells (BCSCs), also named mammospheres. We found that miR-221 was upregulated in BCSCs compared to their differentiated counterpart. Similarly, mammospheres from T47D cells had an increased level of miR-221 compared to differentiated cells. Transfection of miR-221 in T47D cells increased the number of mammospheres and the expression of stem cell markers. Among miR-221's targets, we identified DNMT3b. Furthermore, in BCSCs we found that DNMT3b repressed the expression of various stemness genes, such as Nanog and Oct 3/4, acting on the methylation of their promoters, partially reverting the effect of miR-221 on stemness. We hypothesize that miR-221 contributes to breast cancer tumorigenicity by regulating stemness, at least in part through the control of DNMT3b expression.

  20. Peroxiredoxin 3 is resistant to oxidation-induced apoptosis of Hep-3b cells.

    Science.gov (United States)

    Wang, Y-G; Li, L; Liu, C-H; Hong, S; Zhang, M-J

    2014-06-01

    Although peroxiredoxin 3 (PRX3) was reported to be overexpressed in liver cancer, the precise function of PRX3 in the development and/or progression of liver cancer remained to be obscure. The present study was conducted to investigate the response of PRX3 to oxidative stress in hepatocellular carcinoma (HCC) cells. After successful knockdown of PRX3 expression by small interfering RNA, we treated HCC cell lines Hep-3b and Hep-G2 with gradient concentrations of H2O2 and detected cell proliferation, apoptosis, and the level of reactive oxygen species (ROS) in the cells. After low-dose (5-20 μmol/l) H2O2 treatment, the ROS level was significantly higher in PRX3-knockdown Hep-3b cells than in controls. In addition, PRX3 down-regulation resulted in decreased proliferation, increased apoptosis, and increased caspase 3 activity of Hep-3b cells. We did not notice significant difference between PrxIII knockdown and control Hep-G2 cells in ROS level, cell viability or apoptosis. Our results suggest that PRX3 is an indispensable ROS scavenger that protects tumor cells against oxidative damage and subsequent apoptosis, which provides a clue that PRX3 may be involved in the chemotherapeutic resistance of liver cancer. The underlying mechanism for PRX3 function needs further investigation.

  1. Well data summary sheets. Vol. 20. Danish North Sea

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-03-01

    This twentieth volume of the `Well Data Summary sheets` together with volumes twenty one and twenty two which have been prepared concurrently, includes data from wells in the Danish North Sea with Jurassic sections. With the exception of Falk-1 well in this volume, all the included wells have been released and can be found in the previous volumes (1-16). The primary purpose of producing these three volumes (20-22) has been to update the previously released wells and produce them in our new format. Additionally, this compilation brings together wells from the same geographic region (Central Graben area) with Jurassic sections. An exception to this is the Ibenholt-1 well (volume 21) and the Felicia-1/1A well (this volume). Minor corrections have been made since the first edition. The `Complete index of released wells` had been updated. Volume 27, 28 and 29 are regional volumes; volume 27 includes well data from Southern Jutland; volumes 28 and 29 cover well data from Northern Jutland. All data from released Danish North Sea wells are included in volumes 17-26 and 30. Data references are made to the revised volumes and not to the original published reference. (au)

  2. Anestesia volátil e monitorização anestésica.

    OpenAIRE

    Alexandre, Nuno; Costa, Margarida; Mascarenhas, Ramiro

    2009-01-01

    Os autores apresentam um texto de apoio à disciplina de anestesiologia do curso de Medicina Veterinária .Neste texto são abordadas diversas temáticas da anestesia volátil tais como: equipamentos utilizados em anestesia volátil, farmacologia dos anestésicos voláteis. A monitorização dos pacientes é abordada na vertente mecânica ou intrumental e na componente básica.

  3. Anestesia volátil e monitorização anestésica.

    OpenAIRE

    Alexandre, Nuno; Costa, Margarida; Mascarenhas, Ramiro

    2009-01-01

    Os autores apresentam um texto de apoio à disciplina de anestesiologia do curso de Medicina Veterinária .Neste texto são abordadas diversas temáticas da anestesia volátil tais como: equipamentos utilizados em anestesia volátil, farmacologia dos anestésicos voláteis. A monitorização dos pacientes é abordada na vertente mecânica ou intrumental e na componente básica.

  4. Well data summary sheets. Vol. 30. North Sea wells

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-03-01

    This thirtieth volume of the `Well Data Summary sheets` includes data from three recently released offshore wells from the North Sea. The two wells Amalie-1/1A and Skarv-1 are situated in the Danish Central Graben area. The Ida-1 well is one of a number of Danish North Sea wells situated outside the Central Graben (se also Volume 25). Minor corrections have been made since the first edition. The `Complete index of released wells` had been updated. Volume 27, 28 and 29 are regional volumes; volume 27 includes well data from Southern Jutland; volumes 28 and 29 cover well data from Northern Jutland. All data from released Danish North Sea wells are included in volumes 17-26 and 30. Data references are made to the revised volumes and not to the original published reference. (au)

  5. A linear bound on the tetrahedral number of manifolds of bounded volume (after Jorgensen and Thurston)

    CERN Document Server

    Kobayashi, Tsuyoshi

    2012-01-01

    We provide a detailed proof of the following folklore theorem: Let mu > 0 be a Margulis constant for 3-dimensional hyperbolic space. Then for any d>0 there exists a constant K>0, depending on mu and d, so that for any complete finite volume hyperbolic 3-manifold M, the d-neighborhood of the mu-thick part of M can be triangulated using at most K Vol(M) tetrahedra; here Vol is the hyperbolic volume function. As a corollary, we obtain the following topological interpretation of the volume: the minimal number of tetrahedra required to triangulate a link exterior in M is linearly equivalent to Vol(M); for a precise statement see Corollary 1.3.

  6. The Dnmt3b splice variant is specifically expressed in in vitro-manipulated blastocysts and their derivative ES cells.

    Science.gov (United States)

    Horii, Takuro; Suetake, Isao; Yanagisawa, Eikichi; Morita, Sumiyo; Kimura, Mika; Nagao, Yasumitsu; Imai, Hiroshi; Tajima, Shoji; Hatada, Izuho

    2011-10-01

    Manipulation of preimplantation embryos in vitro, such as in vitro fertilization (IVF), in vitro culture (IVC), intracytoplasmic sperm injection (ICSI), somatic cell nuclear transfer (SCNT) and other assisted reproduction technologies (ART), has contributed to the development of infertility treatment and new animal reproduction methods. However, such embryos often exhibit abnormal DNA methylation patterns in imprinted genes and centromeric satellite repeats. These DNA methylation patterns are established and maintained by three DNA methyltransferases: Dnmt1, Dnmt3a and Dnmt3b. Dnmt3b is responsible for the creation of methylation patterns during the early stage of embryogenesis and consists of many alternative splice variants that affect methylation activity; nevertheless, the roles of these variants have not yet been identified. In this study, we found an alternatively spliced variant of Dnmt3b lacking exon 6 (Dnmt3bΔ6) that is specific to mouse IVC embryos. Dnmt3bΔ6 also showed prominent expression in embryonic stem (ES) cells derived from in vitro manipulated embryos. Interestingly, IVC blastocysts were hypomethylated in centromeric satellite repeat regions that could be susceptible to methylation by Dnmt3b. In vitro methylation activity assays showed that Dnmt3bΔ6 had lower activity than normal Dnmt3b. Our findings suggest that Dnmt3bΔ6 could induce a hypomethylation status especially in in vitro manipulated embryos.

  7. A comprehensive analysis of radiosensitization targets; functional inhibition of DNA methyltransferase 3B radiosensitizes by disrupting DNA damage regulation

    OpenAIRE

    Fujimori, Hiroaki; Sato, Akira; Kikuhara, Sota; Wang, Junhui; Hirai, Takahisa; Sasaki, Yuka; Murakami, Yasufumi; Okayasu, Ryuichi; Masutani, Mitsuko

    2015-01-01

    A comprehensive genome-wide screen of radiosensitization targets in HeLa cells was performed using a shRNA-library/functional cluster analysis and DNMT3B was identified as a candidate target. DNMT3B RNAi increased the sensitivity of HeLa, A549 and HCT116 cells to both γ-irradiation and carbon-ion beam irradiation. DNMT3B RNAi reduced the activation of DNA damage responses induced by γ-irradiation, including HP1β-, γH2AX- and Rad51-foci formation. DNMT3B RNAi impaired damage-dependent H2AX acc...

  8. A comprehensive analysis of radiosensitization targets; functional inhibition of DNA methyltransferase 3B radiosensitizes by disrupting DNA damage regulation

    OpenAIRE

    Fujimori, Hiroaki; Sato, Akira; Kikuhara, Sota; Wang, Junhui; Hirai, Takahisa; Sasaki, Yuka; Murakami, Yasufumi; Okayasu, Ryuichi; Masutani, Mitsuko

    2015-01-01

    A comprehensive genome-wide screen of radiosensitization targets in HeLa cells was performed using a shRNA-library/functional cluster analysis and DNMT3B was identified as a candidate target. DNMT3B RNAi increased the sensitivity of HeLa, A549 and HCT116 cells to both γ3-irradiation and carbon-ion beam irradiation. DNMT3B RNAi reduced the activation of DNA damage responses induced by γ3-irradiation, including HP1β-, γ3H2AX- and Rad51-foci formation. DNMT3B RNAi impaired damage-dependent H2AX ...

  9. DNMT3B 579G>T promoter polymorphism and the risk for idiopathic thrombocytopenic purpura in a Chinese population.

    Science.gov (United States)

    Zhao, Haifeng; Du, Weiting; Gu, Dongsheng; Wang, Donghai; Xue, Feng; Ge, Jing; Sui, Tao; Yang, Renchi

    2009-01-01

    Epigenetics may influence the expression of numerous genes, which might contribute to autoimmune diseases. DNA methylation is mediated by DNA methyltransferases, especially DNA methyltransferase 3B (DNMT3B). Polymorphisms of the DNMT3B gene may influence DNMT3B activity on DNA methylation and increase the susceptibility to several diseases. The current study investigated the association between DNMT3B 579G>T and the risk for idiopathic thrombocytopenic purpura (ITP). The DNMT3B 579G>T polymorphisms were analyzed by PCR-RFLP. There was no significant difference in genotype and allele distribution between the ITP patient and the controls (p = 0.722 and 0.667, respectively). Similar results were observed between the 2 groups when stratified by age and disease course, including acute in childhood, chronic in childhood, acute in adult and chronic in adult. Importantly, this study showed a statistical difference in the distribution of SNP of DNMT3B between Chinese and Koreans or Americans. It is shown that the SNP of DNMT3B 579G>T may not be used on its own as a marker to predict the susceptibility to ITP in a Chinese population and that DNMT3B 579G>T promoter SNP varies from one ethnic population to another.

  10. Comparative proteomic analysis of drug sodium iron chlorophyllin addition to Hep 3B cell line.

    Science.gov (United States)

    Zhang, Jun; Wang, Wenhai; Yang, Fengying; Zhou, Xinwen; Jin, Hong; Yang, Peng-yuan

    2012-09-21

    The human hepatoma 3B cell line was chosen as an experimental model for in vitro test of drug screening. The drugs included chlorophyllin and its derivatives such as fluo-chlorophyllin, sodium copper chlorophyllin, and sodium iron chlorophyllin. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) method was used in this study to obtain the primary screening results. The results showed that sodium iron chlorophyllin had the best LC(50) value. Proteomic analysis was then performed for further investigation of the effect of sodium iron chlorophyllin addition to the Hep 3B cell line. The proteins identified from a total protein extract of Hep 3B before and after the drug addition were compared by two-dimensional-gel-electrophoresis. Then 32 three-fold differentially expressed proteins were successfully identified by MALDI-TOF-TOF-MS. There are 29 unique proteins among those identified proteins. These proteins include proliferating cell nuclear antigen (PCNA), T-complex protein, heterogeneous nuclear protein, nucleophosmin, heat shock protein A5 (HspA5) and peroxiredoxin. HspA5 is one of the proteins which are involved in protecting cancer cells against stress-induced apoptosis in cultured cells, protecting them against apoptosis through various mechanisms. Peroxiredoxin has anti-oxidant function and is related to cell proliferation, and signal transduction. It can protect the oxidation of other proteins. Peroxiredoxin has a close relationship with cancer and can eventually become a disease biomarker. This might help to develop a novel treatment method for carcinoma cancer.

  11. SPITZER IMAGING OF THE NEARBY RICH YOUNG CLUSTER, Cep OB3b

    Energy Technology Data Exchange (ETDEWEB)

    Allen, Thomas S.; Kryukova, Erin; Thomas Megeath, S. [Department of Physics and Astronomy, University of Toledo, Toledo, OH 43606 (United States); Gutermuth, Robert A. [Department of Astronomy, University of Massachusetts, Amherst, MA 01003 (United States); Pipher, Judith L. [Department of Physics and Astronomy, University of Rochester, Rochester, NY 14627 (United States); Naylor, Tim [School of Physics, University of Exeter, Exeter EX4 4QL (United Kingdom); Jeffries, R. D. [Astrophysics Group, School of Physical and Geographical Sciences, Keele University, Keele, Staffordshire ST5 5BG (United Kingdom); Wolk, Scott J.; Spitzbart, Brad [Harvard-Smithsonian Center for Astrophysics, Mail Stop 42, 60 Garden Street, Cambridge, MA 02138 (United States); Muzerolle, James [Space Telescope Science Institute, Baltimore, MD 21218 (United States)

    2012-05-10

    We map the full extent of a rich massive young cluster in the Cep OB3b association with the Infrared Array Camera and Multi-band Imaging Photometer System instruments aboard the Spitzer Space Telescope and the ACIS instrument aboard the Chandra X-Ray Observatory. At 700 pc, it is revealed to be the second nearest large (>1000 member), young (<5 Myr) cluster known. In contrast to the nearest large cluster, the Orion Nebula Cluster, Cep OB3b is only lightly obscured and is mostly located in a large cavity carved out of the surrounding molecular cloud. Our infrared and X-ray data sets, as well as visible photometry from the literature, are used to take a census of the young stars in Cep OB3b. We find that the young stars within the cluster are concentrated in two sub-clusters; an eastern sub-cluster, near the Cep B molecular clump, and a western sub-cluster, near the Cep F molecular clump. Using our census of young stars, we examine the fraction of young stars with infrared excesses indicative of circumstellar disks. We create a map of the disk fraction throughout the cluster and find that it is spatially variable. Due to these spatial variations, the two sub-clusters exhibit substantially different average disk fractions from each other: 32% {+-} 4% and 50% {+-} 6%. We discuss whether the discrepant disk fractions are due to the photodestruction of disks by the high mass members of the cluster or whether they result from differences in the ages of the sub-clusters. We conclude that the discrepant disk fractions are most likely due to differences in the ages.

  12. LC3B is indispensable for selective autophagy of p62 but not basal autophagy

    Energy Technology Data Exchange (ETDEWEB)

    Maruyama, Yoko [Protein Metabolism Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506 (Japan); Department of Pediatrics, School of Medicine, Keio University, Tokyo 160-8582 (Japan); Sou, Yu-Shin; Kageyama, Shun [Protein Metabolism Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506 (Japan); Takahashi, Takao [Department of Pediatrics, School of Medicine, Keio University, Tokyo 160-8582 (Japan); Ueno, Takashi [Division of Proteomics and Biomolecular Science, Center for Biomedical Research Resources, Juntendo University Graduate School of Medicine, Tokyo 113-8421 (Japan); Tanaka, Keiji [Laboratory of Protein Metabolism, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506 (Japan); Komatsu, Masaaki, E-mail: komatsu-ms@igakuken.or.jp [Protein Metabolism Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506 (Japan); Department of Biochemistry, School of Medicine, Niigata University, Niigata 951-8510 (Japan); Ichimura, Yoshinobu, E-mail: ichimura-ys@igakuken.or.jp [Protein Metabolism Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506 (Japan)

    2014-03-28

    Highlights: • Knockdown of LC3 or GABARAP families did not affect the basal autophagy. • LC3B has a higher affinity for the autophagy-specific substrate, p62, than GABARAPs. • siRNA-mediated knockdown of LC3B, but not that of GABARAPs, resulted in significant accumulation of p62. - Abstract: Autophagy is a unique intracellular protein degradation system accompanied by autophagosome formation. Besides its important role through bulk degradation in supplying nutrients, this system has an ability to degrade certain proteins, organelles, and invading bacteria selectively to maintain cellular homeostasis. In yeasts, Atg8p plays key roles in both autophagosome formation and selective autophagy based on its membrane fusion property and interaction with autophagy adaptors/specific substrates. In contrast to the single Atg8p in yeast, mammals have 6 homologs of Atg8p comprising LC3 and GABARAP families. However, it is not clear these two families have different or similar functions. The aim of this study was to determine the separate roles of LC3 and GABARAP families in basal/constitutive and/or selective autophagy. While the combined knockdown of LC3 and GABARAP families caused a defect in long-lived protein degradation through lysosomes, knockdown of each had no effect on the degradation. Meanwhile, knockdown of LC3B but not GABARAPs resulted in significant accumulation of p62/Sqstm1, one of the selective substrate for autophagy. Our results suggest that while mammalian Atg8 homologs are functionally redundant with regard to autophagosome formation, selective autophagy is regulated by specific Atg8 homologs.

  13. Chemical synthesis of Nd2Fe14B/Fe3B nanocomposites.

    Science.gov (United States)

    Yu, L Q; Zhang, Y P; Yang, Z; He, J D; Dong, K T; Hou, Y

    2016-07-14

    High exchange-coupled Nd2Fe14B/Fe3B nanocomposites were synthesized by an integrative procedure of thermal decomposition and reductive annealing processes. The molar ratio of the resulting products of Nd/Fe/B can be tuned by adjusting the raw material proportion. The as-prepared nanocomposites exhibited an exchanged coupled effect with a large coercivity of 11 100 Gs, enhanced remanence Mr of 42.0 emu g(-1), and Mr/M3T of 0.59.

  14. LC3B globular structures correlate with survival in esophageal adenocarcinoma.

    LENUS (Irish Health Repository)

    El-Mashed, Shereen

    2015-01-01

    Esophageal adenocarcinoma has the fastest growing incidence of any solid tumor in the Western world. Prognosis remains poor with overall five-year survival rates under 25 %. Only a limited number of patients benefit from chemotherapy and there are no biomarkers that can predict outcome. Previous studies have indicated that induction of autophagy can influence various aspects of tumor cell biology, including chemosensitivity. The objective of this study was to assess whether expression of the autophagy marker (LC3B) correlated with patient outcome.

  15. Mutation of Breast Cancer Cell Genomic DNA by APOBEC3B

    Science.gov (United States)

    2013-09-01

    histone H3 (1:2,000; Abcam) and anti-tubulin (1:10,000; Covance) followed by anti-mouse 800 or anti- rabbit 680 (1:5,000; Licor) immunoblots were used to...3D-PCR and sequencing. DNA was collected from Ugi-expressing30 T- REx -293 clones or HCC1569 cells transduced with shA3B or shControl lentiviruses. 3D...with Sequencher software (Gene Codes Corporation). Cell cycle experiments. T- REx -293 cells (Invitrogen) were transfected with pcDNA5/TO A3-GFP using

  16. Bandstructure and Fermi Surfaces of CeRh3B2

    OpenAIRE

    2010-01-01

    The electronic bandstructure and the Fermi surfaces of ferromagnetic CeRh3B2 are calculated by using FLAPW and LSDA+U method. As assuming several kinds of the ground state to describe the 4f electronic state, we propose a fully orbital- and spin-polarized state |lz=0, sx=1/2> as the ground state, instead of the conventional LS-coupled CEF ground state, generally expected in typical 4f compounds. This is supported by the fact that both the observed magnetic moment and the observed dHvA frequen...

  17. Thermal expansion and magnetostriction in CeRh3B2

    Science.gov (United States)

    Takeuchi, T.; Thamizhavel, A.; Okubo, T.; Yamada, M.; Inada, Y.; Galatanu, A.; Yamamoto, E.; O¯nuki, Y.

    2004-05-01

    Thermal expansion and magnetostriction measurements have been performed on a single crystal CeRh3B2 with an unusually high Curie temperature TC~120K. The thermal expansion coefficients show a weak anomaly at TC and characteristic positive and negative peaks around 150K for [0001] and [101¯0], respectively. The magnetostriction at 4.2K for the field along the basal plane is very small, with an order of 10-5, suggesting a localized nature of Ce-4f moments.

  18. Variational Mote Carlo Study of Flat Band Ferromagnetism -- Application to CeRh_3 B_2

    OpenAIRE

    2006-01-01

    A new mechanism for ferromagnetism in CeRh_3B_2 is proposed on the basis of variational Monte Carlo results. In a one-dimensional Anderson lattice where each 4f electron hybridizes with a ligand orbital between neighboring Ce sites, ferromagnetism is stabilized due to a nearly flat band which is a mixture of conduction and 4f electron states. Because of the strong spin-orbit interaction in 4f electron states, and of considerable amount of hybridization in the nearly flat band, the magnetic mo...

  19. Coulomb excitation of exotic nuclei at the R3B-LAND setup

    CERN Document Server

    Rossi, D M; Aksouh, F; Alvarez-Pol, H; Aumann, T; Benlliure, J; Böhmer, M; Boretzky, K; Casarejos, E; Chartier, M; Chatillon, A; Cortina-Gil, D; Pramanik, U Datta; Emling, H; Ershova, O; Fernandez-Dominguez, B; Geissel, H; Gorska, M; Heil, M; Johansson, H; Junghans, A; Kiselev, O; Klimkiewicz, A; Kratz, J V; Kurz, N; Labiche, M; Bleis, T Le; Lemmon, R; Litvinov, Yu A; Mahata, K; Maierbeck, P; Movsesyan, A; Nilsson, T; Nociforo, C; Palit, R; Paschalis, S; Plag, R; Reifarth, R; Simon, H; Sümmerer, K; Wagner, A; Walus, W; Weick, H; Winkler, M

    2012-01-01

    Exotic Ni isotopes have been measured at the R3B-LAND setup at GSI in Darmstadt, using Coulomb excitation in inverse kinematics at beam energies around 500 MeV/u. As the experimental setup allows kinematically complete measurements, the excitation energy was reconstructed using the invariant mass method. The GDR and additional low-lying strength have been observed in 68Ni, the latter exhausting 4.1(1.9)% of the E1 energy-weighted sum rule. Also, the branching ratio for the non-statistical decay of the excited 68Ni nuclei was measured and amounts to 24(4)%.

  20. Differential Expression and Clinical Significance of DNA Methyltransferase 3B (DNMT3B), Phosphatase and Tensin Homolog (PTEN) and Human MutL Homologs 1 (hMLH1) in Endometrial Carcinomas.

    Science.gov (United States)

    Li, Wenting; Wang, Ying; Fang, Xinzhi; Zhou, Mei; Li, Yiqun; Dong, Ying; Wang, Ruozheng

    2017-02-21

    BACKGROUND The aim of this study was to investigate the expression and the clinicopathologic significance of DNA methyltransferase 3B (DNMT3B), phosphatase and tensin homolog (PTEN) and human MutL homologs 1 (hMLH1) in endometrial carcinomas between Han and Uygur women in Xinjiang. MATERIAL AND METHODS The expression of DNMT3B, PTEN, and hMLH1 in endometrial carcinomas were assessed by immunohistochemistry, followed by an analysis of their relationship to clinical-pathological features and prognosis. RESULTS There were a 61.7% (95/154) overexpression of DNMT3B, 50.0% (77/154) loss of PTEN expression and 18.2% (28/154) loss of hMLH1 expression. The expression of DNMT3B and PTEN in endometrial carcinomas was statistically significantly different between Uygur women and Han women (p=0.001, p=0.010, respectively). DNMT3B expression was statistically significant based on the grade of endometrial carcinomas (p=0.031). PTEN loss was statistically significant between endometrioid carcinomas (ECs) and non endometrioid carcinomas (NECs) (p=0.040). DNMT3B expression was statistically significant in different myometrial invasion groups in Uygur women (p=0.010). Furthermore, the correlation of DNMT3B and PTEN expression was significant in endometrial carcinomas (p=0.021). PTEN expression was statistically significant in the overall survival (OS) rate of women with endometrial cancers (p=0.041). CONCLUSIONS Our findings suggest that PTEN and DNMT3B possess common regulation features as well as certain ethnic differences in expression between Han women and Uygur women. An interaction may exist in the pathogenesis of endometrial carcinoma. DNMT3B was expressed differently in cases of myometrial invasion and PTEN was associated with OS, which suggested that these molecular markers may be useful in the evaluation of the biological behavior of endometrial carcinomas and may be useful indicators of prognosis in women with endometrial carcinomas.

  1. Index to Nuclear Safety. A technical progress review by chronology, permuted title, and author. Vol. 11, No. 1--Vol. 17, No. 6

    Energy Technology Data Exchange (ETDEWEB)

    Cottrell, W.B.; Klein, A.

    1977-02-23

    This index to Nuclear Safety covers articles in Nuclear Safety Vol. 11, No. 1 (Jan.-Feb. 1970), through Vol. 17, No. 6 (Nov.-Dec. 1976). The index includes a chronological list of articles (including abstract) followed by KWIC and Author Indexes. Nuclear Safety, a bimonthly technical progress review prepared by the Nuclear Safety Information Center, covers all safety aspects of nuclear power reactors and associated facilities. The index lists over 350 technical articles in the last six years of publication.

  2. ON THE TOPOLOGY, VOLUME, DIAMETER AND GAUSS MAP IMAGE OF SUBMANIFOLDS IN A SPHERE

    Institute of Scientific and Technical Information of China (English)

    WU BINGYE

    2004-01-01

    In this paper, the author uses Gauss map to study the topology, volume and diameter of submanifolds in a sphere. It is proved that if there exist ε, 1 ≥ε≥ 0 and a fixed unit simple p-vector a such that the Gauss map g of an n-dimensional complete to Sn, and the volume and diameter of M satisfy εnvol(Sn) ≤vol(M) ≤ vol(Sn)/εand επ≤diam(M) ≤π/ε, respectively. The author also characterizes the case where these inequalities become equalities. As an application, a differential sphere theorem for compact submanifolds in a sphere is obtained.

  3. EphrinB1/EphB3b Coordinate Bidirectional Epithelial-Mesenchymal Interactions Controlling Liver Morphogenesis and Laterality

    DEFF Research Database (Denmark)

    Cayuso, Jordi; Dzementsei, Aliaksandr; Fischer, Johanna C

    2016-01-01

    and adjacent lateral plate mesoderm (LPM), resulting in asymmetric positioning of the zebrafish liver. EphrinB1 in hepatoblasts regulates directional migration and mediates interactions with the LPM, where EphB3b controls polarity and movement of the LPM. EphB3b in the LPM concomitantly repels hepatoblasts...

  4. Association of variation in Fc gamma receptor 3B gene copy number with rheumatoid arthritis in Caucasian samples

    NARCIS (Netherlands)

    McKinney, Cushla; Fanciulli, Manuela; Merriman, Marilyn E.; Phipps-Green, Amanda; Alizadeh, Behrooz Z.; Koeleman, Bobby P. C.; Dalbeth, Nicola; Gow, Peter J.; Harrison, Andrew A.; Highton, John; Jones, Peter B.; Stamp, Lisa K.; Steer, Sophia; Barrera, Pilar; Coenen, Marieke J. H.; Franke, Barbara; van Riel, Piet L. C. M.; Vyse, Tim J.; Aitman, Tim J.; Radstake, Timothy R. D. J.; Merriman, Tony R.

    2010-01-01

    Objective There is increasing evidence that variation in gene copy number (CN) influences clinical phenotype. The low-affinity Fc gamma receptor 3B (FCGR3B) located in the FCGR gene cluster is a CN polymorphic gene involved in the recruitment to sites of inflammation and activation of polymorphonucl

  5. Mutations in sodium channel {beta}-subunit SCN3B are associated with early-onset lone atrial fibrillation

    DEFF Research Database (Denmark)

    Olesen, Morten Salling; Jespersen, Thomas; Nielsen, Jonas Bille

    2011-01-01

    across species. Electrophysiological studies on the SCN3B mutation were carried out and all three SCN3B mutations caused a functionally reduced sodium channel current. One synonymous variant was found in SCN4B. CONCLUSION: In 192 young lone AF patients, we found three patients with suspected disease...

  6. Mutations in sodium channel {beta}-subunit SCN3B are associated with early-onset lone atrial fibrillation

    DEFF Research Database (Denmark)

    Olesen, Morten Salling; Jespersen, Thomas; Nielsen, Jonas Bille

    2011-01-01

    across species. Electrophysiological studies on the SCN3B mutation were carried out and all three SCN3B mutations caused a functionally reduced sodium channel current. One synonymous variant was found in SCN4B. CONCLUSION: In 192 young lone AF patients, we found three patients with suspected disease...

  7. volBrain: an online MRI brain volumetry system

    Directory of Open Access Journals (Sweden)

    Jose V. Manjon

    2016-07-01

    Full Text Available The amount of medical image data produced in clinical and research settings is rapidly growing resulting in vast amount of data to analyze. Automatic and reliable quantitative analysis tools, including segmentation, allow to analyze brain development and to understand specific patterns of many neurological diseases. This field has recently experienced many advances with successful techniques based on non-linear warping and label fusion. In this work we present a novel and fully automatic pipeline for volumetric brain analysis based on multi-atlas label fusion technology that is able to provide accurate volumetric information at different levels of detail in a short time. This method is available through the volBrain online web interface (http://volbrain.upv.es, which is publically and freely accessible to the scientific community. Our new framework has been compared with current state-of-the-art methods showing very competitive results.

  8. Componentes volátiles de mamey (mammea americana L.

    Directory of Open Access Journals (Sweden)

    Alicia Lucía Morales

    2010-07-01

    Full Text Available Los componentes volátiles del aroma de mamey (Mammea americana L, fueron extraídos utilizando el método de destilación por arrastre con vapor-extracción simultánea con solvente orgánico. El extracto fue prefraccionado por cromatografía en columna en silica gel con gradiente discontinuo Pentano: Éter etílico para obtener tres fracciones que fueron analizadas por CGAR y CGAR-EM. Se detectaron 34 compuestos, de los cuales fueron identificados 22, siendo los componentes mayoritarios: Furfural (7281 ^ig/kg y E-Famesol (2145 ng/kg

  9. A spin-orbit alignment for the hot Jupiter HATS-3b

    Energy Technology Data Exchange (ETDEWEB)

    Addison, B. C.; Tinney, C. G.; Wright, D. J. [Exoplanetary Science Group, School of Physics, University of New South Wales, Sydney, NSW 2052 (Australia); Bayliss, D., E-mail: b.addison@unsw.edu.au [Research School of Astronomy and Astrophysics, Australian National University, Canberra, ACT 2611 (Australia)

    2014-09-10

    We have measured the alignment between the orbit of HATS-3b (a recently discovered, slightly inflated Hot Jupiter) and the spin axis of its host star. Data were obtained using the CYCLOPS2 optical-fiber bundle and its simultaneous calibration system feeding the UCLES spectrograph on the Anglo-Australian Telescope. The sky-projected spin-orbit angle of λ = 3° ± 25° was determined from spectroscopic measurements of the Rossiter-McLaughlin effect. This is the first exoplanet discovered through the HATSouth transit survey to have its spin-orbit angle measured. Our results indicate that the orbital plane of HATS-3b is consistent with being aligned to the spin axis of its host star. The low obliquity of the HATS-3 system, which has a relatively hot mid F-type host star, agrees with the general trend observed for Hot Jupiter host stars with effective temperatures >6250 K to have randomly distributed spin-orbit angles.

  10. The superfamily of C3b/C4b-binding proteins

    DEFF Research Database (Denmark)

    Kristensen, Torsten; D'Eustachio, P; Ogata, R T

    1987-01-01

    The determination of primary structures by amino acid and nucleotide sequencing for the C3b-and/or C4b-binding proteins H, C4BP, CR1, B, and C2 has revealed the presence of a common structural element. This element is approximately 60 amino acids long and is repeated in a tandem fashion, commenci......, which have more limited homology with the repetitive regions in this family. All available data indicate that multiple gene duplications and exon shuffling have been important features in the divergence of this family of proteins with the 60-amino-acid repeat.......The determination of primary structures by amino acid and nucleotide sequencing for the C3b-and/or C4b-binding proteins H, C4BP, CR1, B, and C2 has revealed the presence of a common structural element. This element is approximately 60 amino acids long and is repeated in a tandem fashion, commencing...... at the amino-terminal end of each molecule. Two other complement components, C1r and C1s, have two of these repeating units in the carboxy-terminal region of their noncatalytic A chains. Three noncomplement proteins, beta 2-glycoprotein I (beta 2I), the interleukin 2 receptor (IL 2 receptor), and the b chain...

  11. Communal action of microbial cultures for Red HE3B degradation.

    Science.gov (United States)

    Patil, P S; Phugare, S S; Jadhav, S B; Jadhav, J P

    2010-09-15

    The consortium PMB11 consisting of three bacterial species, originally isolated from dye contaminated soil was identified as Bacillus odysseyi SUK3, Morganella morganii SUK5 and Proteus sp. SUK7. The consortium possessed the ability to decolorize various textile dyes as well as mixtures of dyes. PMB11 could decolorize Red HE3B (50 mg l(-1)) with 99% of decolorization within 12 h in nutrient broth, while in mineral medium it could decolorize up to 97% within 24h. Induction in the activities of various oxidative and reductive enzymes indicates the involvement of these enzymes in decolorization. Biodegradation of the dye was monitored using UV-vis spectroscopy, HPLC and FTIR analysis. The Red HE3B degradation pathway was proposed by GC-MS analysis. Various metabolites formed after the degradation were identified as 2,5-diaminobenzene 6-aminotriazine, aniline 2-sulfate, aniline 3-sulfate, 2-amino 5-chlorotriazine and naphthalene. Phytotoxicity studies revealed that metabolites formed after degradation were significantly less toxic in nature.

  12. HATS-3b: An inflated hot Jupiter transiting an F-type star

    CERN Document Server

    Bayliss, D; Penev, K; Bakos, G; Hartman, J; Jordán, A; Mancini, L; Mohler, M; Suc, V; Rabus, M; Béky, B; Csubry, Z; Buchhave, L; Henning, T; Nikolov, N; Csák, B; Brahm, R; Espinoza, N; Noyes, R; Schmidt, B; Conroy, P; Wright, D; Tinney, C; Addison, B; Sackett, P; Sasselov, D; Lázár, J; Papp, I; Sári, P

    2013-01-01

    We report the discovery by the HATSouth survey of HATS-3b, a transiting extrasolar planet orbiting a V=12.4 F-dwarf star. HATS-3b has a period of P = 3.5479d, mass of Mp = 1.07MJ, and radius of Rp = 1.38RJ. Given the radius of the planet, the brightness of the host star, and the stellar rotational velocity (vsini = 9.0km/s), this system will make an interesting target for future observations to measure the Rossiter-McLaughlin effect and determine its spin-orbit alignment. We detail the low/medium-resolution reconnaissance spectroscopy that we are now using to deal with large numbers of transiting planet candidates produced by the HATSouth survey. We show that this important step in discovering planets produces logg and Teff parameters at a precision suitable for efficient candidate vetting, as well as efficiently identifying stellar mass eclipsing binaries with radial velocity semi-amplitudes as low as 1 km/s.

  13. EEG-correlated fMRI of P3b component in P300 waves

    Institute of Scientific and Technical Information of China (English)

    LI Yuezhi; WANG Liqun; WANG Mingshi

    2005-01-01

    Electroencephalography-correlated functional magnetic resonance imaging (EEG/fMRI) can be used to identify blood oxygen level-dependent (BOLD) signal changes associated with both physiological and pathological EEG events. Here, we implemented continuous and simultaneous EEG/fMRI to identify BOLD signal changes related to P3b component of P300, and 64 channels of EEG were recorded in 11 subjects during Landot Ring task inside a 1.5 T functional magnet resonance (MR) scanner using an MR-compatible EEG recording system. Functional scanning by echoplanar imaging covered almost the entire cerebrum every 2 s, leaving gaps of 2 s without scanning. Off-line MRI artifact subtraction software was applied to obtain continuous EEG data. Additionally, a P300 wave matched filter was constructed to inspect P300 wave occurrence following every target stimulus, target stimuli inspected to induce P300 were detected and their MRI scan number were then used as input for an event-related fMRI analysis. Finally MRI statistical parametric maps were constructed and corrected for multiple comparisons. By random effect group analysis, activations were detected in the right superior parietal lobule and bilaterally in inferior parietal lobule(p<0.001, uncorrected). The results demonstrated the upper regions were sources of P3b component and involved in target detection in memory comparison task.

  14. Embryonic expression of zebrafish MiT family genes tfe3b, tfeb, and tfec.

    Science.gov (United States)

    Lister, James A; Lane, Brandon M; Nguyen, Anhthu; Lunney, Katherine

    2011-11-01

    The MiT family comprises four genes in mammals: Mitf, Tfe3, Tfeb, and Tfec, which encode transcription factors of the basic-helix-loop-helix/leucine zipper class. Mitf is well-known for its essential role in the development of melanocytes, however the functions of the other members of this family, and of interactions between them, are less well understood. We have now characterized the complete set of MiT genes from zebrafish, which totals six instead of four. The zebrafish genome contain two mitf (mitfa and mitfb), two tfe3 (tfe3a and tfe3b), and single tfeb and tfec genes; this distribution is shared with other teleosts. We present here the sequence and embryonic expression patterns for the zebrafish tfe3b, tfeb, and tfec genes, and identify a new isoform of tfe3a. These findings will assist in elucidating the roles of the MiT gene family over the course of vertebrate evolution. Copyright © 2011 Wiley-Liss, Inc.

  15. TASTE. III. A homogeneous study of transit time variations in WASP-3b

    CERN Document Server

    Nascimbeni, V; Murabito, S; Sada, P V; Aparicio, A; Piotto, G; Bedin, L R; Milone, A P; Rosenberg, A; Borsato, L; Damasso, M; Granata, V; Malavolta, L

    2012-01-01

    The TASTE project is searching for low-mass planets with the Transit Timing Variation (TTV) technique, by gathering high-precision, short-cadence light curves for a selected sample of transiting exoplanets. It has been claimed that the "hot Jupiter" WASP-3b could be perturbed by a second planet. Presenting eleven new light curves (secured at the IAC80 and UDEM telescopes) and re-analyzing thirty-eight archival light curves in a homogeneous way, we show that new data do not confirm the previously claimed TTV signal. However, we bring evidence that measurements are not consistent with a constant orbital period, though no significant periodicity can be detected. Additional dynamical modeling and follow-up observations are planned to constrain the properties of the perturber or to put upper limits to it. We provide a refined ephemeris for WASP-3b and improved orbital/physical parameters. A contact eclipsing binary, serendipitously discovered among field stars, is reported here for the first time.

  16. HATS-3b: AN INFLATED HOT JUPITER TRANSITING AN F-TYPE STAR

    Energy Technology Data Exchange (ETDEWEB)

    Bayliss, D.; Zhou, G.; Schmidt, B. [Research School of Astronomy and Astrophysics, Australian National University, Canberra, ACT 2611 (Australia); Penev, K.; Bakos, G. Á.; Hartman, J. D.; Csubry, Z. [Department of Astrophysical Sciences, Princeton University, NJ 08544 (United States); Jordán, A.; Suc, V.; Rabus, M.; Brahm, R.; Espinoza, N. [Departamento de Astronomía y Astrofísica, Pontificia Universidad Católica de Chile, Av. Vicuña Mackenna 4860, 7820436 Macul, Santiago (Chile); Mancini, L.; Mohler-Fischer, M.; Henning, T.; Nikolov, N.; Csák, B. [Max Planck Institute for Astronomy, Heidelberg (Germany); Béky, B.; Noyes, R. W. [Harvard-Smithsonian Center for Astrophysics, Cambridge, MA (United States); Buchhave, L., E-mail: daniel@mso.anu.edu.au [Niels Bohr Institute, Copenhagen University (Denmark); and others

    2013-11-01

    We report the discovery by the HATSouth survey of HATS-3b, a transiting extrasolar planet orbiting a V = 12.4 F dwarf star. HATS-3b has a period of P = 3.5479 days, mass of M{sub p} = 1.07 M {sub J}, and radius of R{sub p} = 1.38 R {sub J}. Given the radius of the planet, the brightness of the host star, and the stellar rotational velocity (vsin i = 9.0 km s{sup –1}), this system will make an interesting target for future observations to measure the Rossiter-McLaughlin effect and determine its spin-orbit alignment. We detail the low-/medium-resolution reconnaissance spectroscopy that we are now using to deal with large numbers of transiting planet candidates produced by the HATSouth survey. We show that this important step in discovering planets produces log g and T {sub eff} parameters at a precision suitable for efficient candidate vetting, as well as efficiently identifying stellar mass eclipsing binaries with radial velocity semi-amplitudes as low as 1 km s{sup –1}.

  17. CALIFA, a Dedicated Calorimeter for the R{sup 3}B/FAIR

    Energy Technology Data Exchange (ETDEWEB)

    Cortina-Gil, D., E-mail: d.cortina@usc.es [Universidad de Santiago de Compostela, E-15782 Santiago de Compostela (Spain); Alvarez-Pol, H. [Universidad de Santiago de Compostela, E-15782 Santiago de Compostela (Spain); Aumann, T. [Technische Universität Darmstadt, D-64289 Darmstadt (Germany); Helmholtzzentrum für Schwerionenforschung, D-64291 Darmstadt (Germany); Avdeichikov, V. [University of Lund, SE 221 00 Lund (Sweden); Bendel, M. [Technische Universität München, D-85748 Garching (Germany); Benlliure, J. [Universidad de Santiago de Compostela, E-15782 Santiago de Compostela (Spain); Bertini, D. [Helmholtzzentrum für Schwerionenforschung, D-64291 Darmstadt (Germany); Bezbakh, A. [Joint Institute for Nuclear Research, RU-141980 Dubna (Russian Federation); Bloch, T. [Technische Universität Darmstadt, D-64289 Darmstadt (Germany); Böhmer, M. [Technische Universität München, D-85748 Garching (Germany); Borge, M.J.G.; Briz, J.A. [Instituto Estructura de la Materia, CSIC, E-28006 Madrid (Spain); Cabanelas, P. [Universidad de Santiago de Compostela, E-15782 Santiago de Compostela (Spain); Casarejos, E. [Universidad de Vigo, E-36310 Vigo (Spain); Carmona Gallardo, M. [Instituto Estructura de la Materia, CSIC, E-28006 Madrid (Spain); Cederkäll, J. [University of Lund, SE 221 00 Lund (Sweden); Chulkov, L. [Nuclear Reseach Center, Kurchatov Institute, RU-123182 Moscow (Russian Federation); Dierigl, M. [Technische Universität München, D-85748 Garching (Germany); Di Julio, D. [University of Lund, SE 221 00 Lund (Sweden); Durán, I. [Universidad de Santiago de Compostela, E-15782 Santiago de Compostela (Spain); and others

    2014-06-15

    The R{sup 3}B experiment (Reactions with Relativistic Radioactive Beams) at FAIR (Facility for Antiproton and Ion Research) is a versatile setup dedicated to the study of reactions induced by high-energy radioactive beams. It will provide kinematically complete measurements with high efficiency, acceptance and resolution, making possible a broad physics program with rare-isotopes. CALIFA (CALorimeter for In-Flight detection of gamma-rays and high energy charged pArticles), is a complex detector based on scintillation crystals, that will surround the target of the R{sup 3}B experiment. CALIFA will act as a total absorption gamma-calorimeter and spectrometer, as well as identifier of charged particles from target residues. This versatility is its most challenging requirement, demanding a huge dynamic range, to cover from low energy gamma-rays up to 300 MeV protons. This fact, along with the high-energy of the beams determine the conceptual design of the detector, presented in this paper, together with the technical solutions proposed for its construction.

  18. a Bayesian Approach for Calibration of Trmm 3B42 Over North Amazonia

    Science.gov (United States)

    Linguet, L.; Marie-Joseph, I.; Becker, M.; Seyler, F.

    2013-12-01

    Northern Amazonian regions experience extremes conditions like floods and droughts. These regions are also characterized by the limited spatial coverage of ground based rain gauges, and unavailability of real-time rainfall data. Satellite-based rainfall estimates (SRE) may be one of the best and appropriate approaches in detecting rainfall distribution. However SRE data need specific calibration and validation for use in flood and drought monitoring activities. This study aimed to calibrate of TRMM 3B42 RT rainfall products over northern Amazonia with a Bayesian filtering approach [1] [2]. The study area is located north of the Amazon River and includes the three Guianas and northern states of Brazil. A set of daily satellite rainfall products with spatial resolution of 0.25°x0.25° (TRMM 3B42 RT) from the year 2000 to 2010 has been selected. Ground reference data are located in French Guiana (27 ground stations from French national meteorological agency) and in the northern Brazilian states (70 ground stations from Brazilian Agência Nacional de Aguas). A lot of bias-adjustment methods rely on computing the difference between satellite and gauge-based precipitation [3] [4]. In this study we defend the idea that an inverse approach based on sequential Monte Carlo filtering helps to calibrate of TRMM 3B42 RT rainfall products. The developed method combines a model of the rainfall process at rain gauge locations with a stochastic observation model based on the joint distribution between ground reference data of the state variable (rainfall data) and the observed satellite data. 50% of the total ground based rainfall measurements were used for the joint distribution and the remaining 50% were used for validation purposes. Validation of the method has been done by comparing the corrected satellite data against independent observed data from rain gauges using the standard verification techniques: mean bias error, root mean square error, and correlation coefficient

  19. Downregulation of a newly identified laminin, laminin-3B11, in vascular basement membranes of invasive human breast cancers.

    Science.gov (United States)

    Mori, Taizo; Kariya, Yoshinobu; Komiya, Eriko; Higashi, Shouichi; Miyagi, Yohei; Sekiguchi, Kiyotoshi; Miyazaki, Kaoru

    2011-05-01

    Laminins present in the basement membranes (BM) of blood vessels are involved in angiogenesis and other vascular functions that are critical for tumor growth and metastasis. Two major vascular laminins, the α4 (laminin-411/421) and α5 (laminin-511/521) types, have been well characterized. We recently found a third type of vascular laminin, laminin-3B11, consisting of the α3B, β1 and γ1 chains, and revealed its biological activity. Laminin-3B11 potently stimulates vascular endothelial cells to extend lamellipodial protrusions. To understand the roles of laminin-3B11 in blood vessel functions and tumor growth, we examined localization of the laminin α3B chain in normal mammary glands and breast cancers, in comparison with the α4 and α5 laminins. In the immunohistochemical analysis, the α3B laminin was co-localized with the α4 and α5 laminins in the BM of venules and capillaries of normal breast tissues, but α3B was scarcely detected in vessels near invasive breast carcinoma cells. In contrast, the α4 laminin was overexpressed in capillaries of invasive carcinomas, where a large number of macrophages were found. The α5 laminin appeared to be weakly downregulated in cancer tissues, especially in capillary vessels. Furthermore, our in vitro analysis indicated that TNF-α significantly suppressed the laminin α3B expression in vascular endothelial cells, while it, as well as IL-1β and TGF-α, upregulated the α4 expression. These results suggest that Lm3B11/3B21 may be required for normal mature vessels and interfere with tumor angiogenesis.

  20. Atrial and ventricular volume and function in persistent and permanent atrial fibrillation, a magnetic resonance imaging study

    DEFF Research Database (Denmark)

    Therkelsen, Susette Krohn; Groenning, Bjoern Aaris; Svendsen, Jesper Hastrup;

    2005-01-01

    . Intraobserver variability and inter-study reproducibility of the cardiac volumes and ejection fractions (EF) gave acceptable Bland-Altman plots, good correlations (R2: 0.80-0.99), and low reproducibility coefficients. The mean atrial volumes were similar in the two groups with AF [systolic vol. index (SVI): 75...

  1. Heparan sulfate D-glucosaminyl 3-O-sulfotransferase-3B1 (HS3ST3B1) promotes angiogenesis and proliferation by induction of VEGF in acute myeloid leukemia cells.

    Science.gov (United States)

    Zhang, Lei; Song, Kai; Zhou, Ling; Xie, Zhishen; Zhou, Ping; Zhao, Yiming; Han, Yue; Xu, Xiaojun; Li, Ping

    2015-06-01

    Heparan sulfate (HS) are complex polysaccharides that reside on the plasma membrane of almost all mammalian cells, and play an important role in physiological and pathological conditions. Heparan sulfate D-glucosamine 3-O-sulfotransferase 3B1 (HS3ST3B1) participates in the last biosynthetic steps of HS and transfers sulfate to the 3-O-position of glucosamine residues to yield mature sugar chains. To date very few biological processes or proteins have been described that are modulated by HS3ST3B1. In this study, we observed that HS3ST3B1 positively contributed to acute myeloid leukemia (AML) progression in vitro and in vivo, and these activities were associated with an induction of the proangiogenic factor VEGF expression and shedding. Moreover, the effects of HS3ST3B1 on VEGF release can be attenuated after treatment of heparanase inhibitor suramin, which prevented VEGF secretion and subsequently blocked VEGF-induced activation of ERK and AKT, suggesting that 3-O-sulfation of HS by HS3ST3B1 facilitated VEGF shedding; the effects of HS3ST3B1 on activation of ERK and AKT can also be blocked by VEGFR inhibitor axitinib, suggestive of a relationship between 3-O-sulfation of HS and VEGF-activated signaling pathways. Taken together, our findings support that VEGF is an important functional target of HS3ST3B1 and provide a new mechanism of HS3ST3B1 in AML.

  2. 子宫内膜癌中 DNA 甲基转移酶3B 的表达特点与临床意义%Characteristic and clinical significance of DNA methyltransferase 3B overexpression in endometrial carcinoma

    Institute of Scientific and Technical Information of China (English)

    董颖; 周梅; 巴晓军; 司婧文; 李文婷; 王颖; 李东; 李挺

    2016-01-01

    Objective:To determine the clinicopathological significance of the DNA methyltransferase 3B (DNMT3B)overexpression in endometrial carcinomas and to evaluate its correlation with hormone re-ceptor status.Methods:Immunohistochemistry was performed to assess the expression of DNMT3B and hormone receptors in 104 endometrial carcinomas.Results:DNMT3B overexpression occurred frequently in endometrioid carcinoma (EC,54.8%)more than in nonendometrioid carcinoma (NEC,30.0%) with statistical significance (P =0.028).Furthermore,there was a trend that EC with worse clinico-pathological variables and shorter survival had a higher DNMT3B expression,and the correlation between DNMT3B and tumor grade reached statistical significance (P =0.019).A negative correlation between DNMT3B and estrogen receptor (ER)or progesterone receptor (PR)expression was found in EC. NMT3B overexpression occurred frequently in the ER or PR negative subgroups (78.9%,86.7%)more than in the positive subgroups (47.7%,47.8%)with statistical significance (P =0.016,P =0.006). In addition,the DNMT3B overexpression increased in tumors with both ER and PR negative expression (92.9%,P =0.002).However,no such correlation was found in NEC (P >0.05).Sequence analyses demonstrated multiple ER and PR binding sites in the promoter regions of DNMT3B gene.Conclusion:This study showed that the expression of DNMT3B in EC and NEC was different.DNMT3B overexpres-sion in EC was associated with the worse clinicopathological variables and might have predictive value. The methylation status of EC and NEC maybe different.In addition,in EC,DNMT3B overexpression negatively correlated with ER or PR expression.In NEC,the correlation between DNMT3B and ER or PR status was not present.%目的:探讨子宫内膜癌中 DNA 甲基转移酶3B(DNA methyltransferase 3B,DNMT3B)的表达特点、与雌激素受体(estrogen receptor,ER)和孕激素受体(progesterone receptor,PR)的相关性及意义。方法

  3. Precipitation comparison for the CFSR, MERRA, TRMM3B42 and Combined Scheme datasets in Bolivia

    Science.gov (United States)

    Blacutt, Luis A.; Herdies, Dirceu L.; de Gonçalves, Luis Gustavo G.; Vila, Daniel A.; Andrade, Marcos

    2015-09-01

    An overwhelming number of applications depend on reliable precipitation estimations. However, over complex terrain in regions such as the Andes or the southwestern Amazon, the spatial coverage of rain gauges is scarce. Two reanalysis datasets, a satellite algorithm and a scheme that combines surface observations with satellite estimations were selected for studying rainfall in the following areas of Bolivia: the central Andes, Altiplano, southwestern Amazonia, and Chaco. These Bolivian regions can be divided into three main basins: the Altiplano, La Plata, and Amazon. The selected reanalyses were the Modern-Era Retrospective Analysis for Research and Applications, which has a horizontal resolution (~ 50 km) conducive for studying rainfall in relatively small precipitation systems, and the Climate Forecast System Reanalysis and Reforecast, which features an improved horizontal resolution (~ 38 km). The third dataset was the seventh version of the Tropical Rainfall Measurement Mission 3B42 algorithm, which is conducive for studying rainfall at an ~ 25 km horizontal resolution. The fourth dataset utilizes a new technique known as the Combined Scheme, which successfully removes satellite bias. All four of these datasets were aggregated to a coarser resolution. Additionally, the daily totals were calculated to match the cumulative daily values of the ground observations. This research aimed to describe and compare precipitations in the two reanalysis datasets, the satellite-algorithm dataset, and the Combined Scheme with ground observations. Two seasons were selected for studying the precipitation estimates: the rainy season (December-February) and the dry season (June-August). The average, bias, standard deviation, correlation coefficient, and root mean square error were calculated. Moreover, a contingency table was generated to calculate the accuracy, bias frequency, probability of detection, false alarm ratio, and equitable threat score. All four datasets correctly

  4. DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines

    Directory of Open Access Journals (Sweden)

    Rivenbark Ashley G

    2008-01-01

    Full Text Available Abstract Background DNA hypermethylation events and other epimutations occur in many neoplasms, producing gene expression changes that contribute to neoplastic transformation, tumorigenesis, and tumor behavior. Some human cancers exhibit a hypermethylator phenotype, characterized by concurrent DNA methylation-dependent silencing of multiple genes. To determine if a hypermethylation defect occurs in breast cancer, the expression profile and promoter methylation status of methylation-sensitive genes were evaluated among breast cancer cell lines. Results The relationship between gene expression (assessed by RT-PCR and quantitative real-time PCR, promoter methylation (assessed by methylation-specific PCR, bisulfite sequencing, and 5-aza-2'deoxycytidine treatment, and the DNA methyltransferase machinery (total DNMT activity and expression of DNMT1, DNMT3a, and DNMT3b proteins were examined in 12 breast cancer cell lines. Unsupervised cluster analysis of the expression of 64 methylation-sensitive genes revealed two groups of cell lines that possess distinct methylation signatures: (i hypermethylator cell lines, and (ii low-frequency methylator cell lines. The hypermethylator cell lines are characterized by high rates of concurrent methylation of six genes (CDH1, CEACAM6, CST6, ESR1, LCN2, SCNN1A, whereas the low-frequency methylator cell lines do not methylate these genes. Hypermethylator cell lines coordinately overexpress total DNMT activity and DNMT3b protein levels compared to normal breast epithelial cells. In contrast, most low-frequency methylator cell lines possess DNMT activity and protein levels that are indistinguishable from normal. Microarray data mining identified a strong cluster of primary breast tumors that express the hypermethylation signature defined by CDH1, CEACAM6, CST6, ESR1, LCN2, and SCNN1A. This subset of breast cancers represents 18/88 (20% tumors in the dataset analyzed, and 100% of these tumors were classified as basal

  5. [Operative treatment of Dubberley type 3B capitulum-trochlea fractures].

    Science.gov (United States)

    Hu, Xiaochuan; Xiang, Ming; Yang, Guoyong; Chen, Hang; Tang, Haochen

    2014-10-01

    To investigate the method and effectiveness of operative treatment of Dubberley type 3B capitulum-trochlea fractures. Between January 2009 and December 2012, 8 cases of Dubberley type 3B capitulum- trochlea fractures were treated. There were 2 males and 6 females with an average age of 55 years (range, 43-65 years). The injury was caused by falling in 6 cases, electric bicycle accident in 1 case, and traffic accident in 1 case. All fractures were fresh and closed injury. No neural or vascular injury was found. The time between injury and operation was 3-15 days (mean, 5.9 days). Olecranon osteotomy was performed by a posterior midline skin incision of the elbow; 3.0 mm Herbert compression screws placed from posterior to anterior, 2.4 mm L shape locking compression plate designed for distal radius or 2.7 mm anatomical locking compression plate designed for distal humerus and 1.0 mm Kirschner wires or 3.0 mm Herbert screw for the transverse and coronal plane in the subchondral of anterior articular surface were used for fixation; and the lateral and medial collateral ligaments were repaired. All incisions healed by first intention. The patients were followed up 12-18 months (mean, 14.5 months). The X-ray films showed that fracture healing was achieved at 12-24 weeks (mean, 15 weeks) in 7 cases. Fracture nonunion and partial bone resorption in the capitellum were observed in 1 case. No failure of internal fixation, ulnohumeral joint instability, or traumatic arthritis occurred. At last follow-up, the range of motion of injured elbow was 0-40° in extension (mean 25.0°), 100-135° in flexion (mean, 116.3°), 60-70° in pronation (mean, 61.3°), and 80-90° in supination (mean, 81.3°). The elbow function score was 64-96 (mean, 81.1) according to the Broberg-Morrey evaluation criteria; the results were excellent in 2 cases, good in 4 cases, and fair in 2 cases with an excellent and good rate of 75%. The visual analogue scale (VAS) score was 0-3 (mean, 1). For Dubbelley

  6. let-7 Modulates Chromatin Configuration and Target Gene Repression through Regulation of the ARID3B Complex

    Directory of Open Access Journals (Sweden)

    Tsai-Tsen Liao

    2016-01-01

    Full Text Available Let-7 is crucial for both stem cell differentiation and tumor suppression. Here, we demonstrate a chromatin-dependent mechanism of let-7 in regulating target gene expression in cancer cells. Let-7 directly represses the expression of AT-rich interacting domain 3B (ARID3B, ARID3A, and importin-9. In the absence of let-7, importin-9 facilitates the nuclear import of ARID3A, which then forms a complex with ARID3B. The nuclear ARID3B complex recruits histone demethylase 4C to reduce histone 3 lysine 9 trimethylation and promotes the transcription of stemness factors. Functionally, expression of ARID3B is critical for the tumor initiation in let-7-depleted cancer cells. An inverse association between let-7 and ARID3A/ARID3B and prognostic significance is demonstrated in head and neck cancer patients. These results highlight a chromatin-dependent mechanism where let-7 regulates cancer stemness through ARID3B.

  7. Flying Qualities Flight Testing of Digital Flight Control Systems. Flight Test Techniques Series - Volume 21 (les Essais en vol des performances des systemes de ommande de vol numeriques)

    Science.gov (United States)

    2001-12-01

    et leur sécurité. Note de traduction : l’auteur insiste lourdement dans le 2ème paragraphe sur la préparation des essais et l’analyse des...feedback loop is readily apparent. As an example, a roll-rate feedback to the aileron is a good indication that the roll mode required augmentation. The...impacts of roll-rate feedback to the aileron on this mode are well understood from the classical design theory. Newer or so-called modern design

  8. The time development of hadronic showers and the T3B experiment

    Energy Technology Data Exchange (ETDEWEB)

    Soldner, Christian

    2013-06-06

    The compact linear collider (CLIC) is a future linear e{sup +}e{sup -} collider operated at a center of mass energy of up to 3 TeV and with a collision rate of particle bunches of up to 2 GHz. This poses challenging requirements on the detector system. The accumulation of background events, such as {gamma}{gamma}{yields}hadrons resulting from Beamstrahlung, must be minimized through a precise time stamping capability in all subdetector systems. In the event reconstruction, the energy depositions within the calorimeters will be used to assign events precisely to a small set of consecutive bunch crossings. The finite time evolution of hadronic showers, on the other hand, requires an extended integration time to achieve a satisfactory energy resolution in the calorimeter. The energy resolution is also deteriorated by the leakage of shower particles. Tungsten is foreseen as dense absorber material, but the time evolution of hadron showers within such a calorimeter is not sufficiently explored yet. In the context of this thesis, the T3B experiment (short for Tungsten Timing Test Beam) was designed and constructed. It is optimized to measure the time development and the contribution of delayed energy depositions within hadronic cascades. The T3B experiment consists of 15 scintillator cells assembled in a strip. The scintillation light generated within the cells is detected by novel silicon photomultiplier whose signal is read out with fast oscilloscopes providing a sampling rate of 1.25 GHz. This strip was positioned behind two different calorimeter prototypes of the CALICE collaboration which use a tungsten and steel (for comparison) absorber structure. T3B was part of the CALICE test beam campaign 2010/2011 carried out at the PS and SPS at CERN and acquired data on hadronic showers in an energy range of 2-300 GeV. A test beam optimized data acquisition software was developed from scratch. With the development and application of a novel waveform decomposition algorithm

  9. Preparation of integrated multifunction Pb3B10O16[OH]4 whisker by solvothermal method

    Science.gov (United States)

    Sun, Qing; Zhang, Quan-Ping; Zheng, Jian; Zhou, Dong; Li, Yin-Tao; Zhou, Yuan-Lin

    2016-03-01

    Elaborate design of multifunction materials is of great scientific and technological significance; but it is a great challenge. Here, a lead borate is successfully prepared via a facile solvothermal method. The results of XRD, SEM and TEM show the product is a kind of whiskers with uniform structure and high length-diameter ratio, which is represented as Pb3B10O16[OH]4. The whisker is capable of attenuating both γ-rays and neutrons and shows a little difference with that of the equal molar mass of Pb and B in mixture. In addition, the whisker displays good photoluminescence properties, especially for luminescent intensity. These significant results indicate an integrated multifunction whisker that will stimulate new application research.

  10. Selenium regulation of glutathione peroxidase in human hepatoma cell line Hep3B.

    Science.gov (United States)

    Baker, R D; Baker, S S; LaRosa, K; Whitney, C; Newburger, P E

    1993-07-01

    Glutathione peroxidase is an important enzyme in cellular antioxidant defense systems, detoxifying peroxides and hydroperoxides. As a component of the glutathione cycle, it protects the liver from reactive oxygen metabolites. Selenocysteine is present at the catalytic site of glutathione peroxidase, and selenium availability regulates glutathione peroxidase enzyme activity. Hep3B cells, a well-differentiated human hepatoma-derived cell line, exhibited time-dependent decrease in glutathione peroxidase activity (nmol NADPH oxidized/min/mg protein, mean +/- SE) when incubated in selenium-free medium for 10 days (Day 0, 21.8 +/- 7.3; Day 2, 10.9 +/- 1.2; Day 4, 7.9 +/- 0.8; Day 6, 4.0 +/- 0.7; Day 8, 4.5 +/- 0.6; Day 10, 1.6 +/- 0.4). With the reintroduction of selenium, glutathione peroxidase activity returned. A second human hepatoma cell line, HepG2, demonstrated a similar pattern when depleted of and then repleted with selenium. To assess protein synthesis, glutathione peroxidase activity was measured in deficient and replete Hep3B cells incubated with and without selenium and with and without cycloheximide. Deficient cells (mean +/- SE) (4.9 +/- 0.2) showed an increase in glutathione peroxidase activity after 24 h in selenium-containing medium (11.6 +/- 0.2), but not when cycloheximide was included in the medium (6.9 +/- 0.5) or when cycloheximide and no selenium was included (5.3 +/- 0.8). Replete Hep3B cells (40.1 +/- 1.1) demonstrated decreased glutathione peroxidase after 24 h in medium without selenium (34.0 +/- 1.4), medium with both cycloheximide and selenium (34.0 +/- 2.6), and medium without selenium and containing cycloheximide (37.6 +/- 1.3). These data suggest that protein synthesis is needed for selenium repletion to exert control on glutathione peroxidase activity. Using a cDNA for human glutathione peroxidase (GPx1), selenium-deficient and replete Hep3B cell RNA was analyzed by Northern blot. mRNA for GPx was quantified by densitometry. The steady

  11. Ecological toxicity of reactive X-3B red dye and cadmium acting on wheat (Triticum aestivum)

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Ecological toxicity of reactive X-3B red dye and cadmium in both their single form and their combined form on wheat was studied using the experimental method of seed and root exposure. The single-factor exposure indicated that the inhibitory rate of wheat root elongation was significantly increased with the increase in the concentration of the dye in the cultural solution, although seed germination of wheat was not sensitive to the dye. The toxicity of cadmium was greatly higher than that of the dye, but low concentration cadmium (< 40 mg/L) could promote the germination of wheat seed. Interactive effects of the dye and cadmium on wheat were complicated. There was no significant correlation between the inhibitory rate of seed germination and the concentrations of the dye and cadmium. Low concentration cadmium could strengthen the toxicity of the dye acting on root elongation. On the contrary, high concentration cadmium could weaken the toxicity of the dye acting on root elongation.

  12. Mobilities and dislocation energies of planar faults in an ordered A3B (D019) structure

    Indian Academy of Sciences (India)

    A K Singh; R Sankarasubramanian; T K Nandy

    2013-08-01

    Present work describes the stability of possible planar faults of the A3B (D019) phase with an axial ratio less than the ideal. Mobilities and dislocation energies of various planar faults viz. antiphase boundaries (APBs), superlattice intrinsic stacking faults (SISFs) and complex stacking faults (CSFs) have been computed using complex fourth-order tensor transformations and hard sphere model. Displacements normal to the slip planes for various slip systems (vertical shift) have been used to calculate mobility of dislocations. The energy of the planar faults in Ti3Al intermetallic is calculated using some simplifying assumptions. Based on the mobility and energy, stability of planar faults has been explained. These results are compared with single crystal ordered Ti3Al alloy having D019 structure.

  13. Valence state of Ce and the magnetism in CeRh3B2

    Science.gov (United States)

    Malik, S. K.; Umarji, A. M.; Shenoy, G. K.; Montano, P. A.; Reeves, M. E.

    1985-04-01

    The compound CeRh3B2 orders magnetically with a high Curie temperature (TC) of 115 K but with a low moment of only absorption edge measurements show a dominant absorption peak at the energy corresponding to trivalent cerium. Magnetic studies on the compounds Ce(Rhsub1-xTsubx)sub3Bsub2 with T=Ru and Os reveal that the magnetic state is very rapidly broken up with the replacement of Rh by Ru and Os. These results along with the observation of a high TsubC in CeRhsub3Bsub2 suggest that magnetism in this compound arises from a strong hybridization of nearly localized or slightly delocalized Ce 4f electrons with conduction electrons. The small moment may be due to a Kondo-type interaction coupled with crystal-field effects.

  14. Bandstructure and Fermi Surfaces of CeRh3B2

    Science.gov (United States)

    Yamauchi, Kunihiko; Yanase, Akira; Harima, Hisatomo

    2010-04-01

    The electronic bandstructure and the Fermi surfaces of ferromagnetic CeRh3B2 are calculated by using FLAPW and LSDA+U method. As assuming several kinds of the ground state to describe the 4f electronic state, we propose a fully orbital- and spin-polarized state | lz=0, sx=1/2 > as the ground state, instead of the conventional \\mathit{LS}-coupled CEF ground state, generally expected in typical 4f compounds. This is supported by the fact that both the observed magnetic moment and the observed dHvA frequencies are well explained by the calculated electronic structure and the Fermi surfaces. The unconventional ground state is stabilized by the strong 4f-4f direct mixing between the neighbored Ce atoms along the extremely small distance along the c-axis in the hexagonal crystal cell.

  15. Photoemission study of the ferromagnetic Kondo system CeRh3B2

    Science.gov (United States)

    Fujimori, A.; Takahashi, T.; Okabe, A.; Kasaya, M.; Kasuya, T.

    1990-04-01

    We have studied the electronic structure of CeRh3B2, which has an anomalously high ferromagnetic ordering temperature, by photoemission and Auger-electron spectroscopy. The Ce 4f occupancy nf~=0.85 evaluated from the Ce 3d core-level photoemission spectrum indicates a moderately strong valence fluctuation in the Kondo regime. Rh d-derived valence-band photoemission spectra are found to be in good agreement with the results of band-structure calculations when a strong energy dependence of the hole lifetime is taken into account. This observation and the deviation of the Rh M4,5VV Auger spectrum from the self-convolution of the Rh d partial density of states provide evidence for electron correlation within the Rh d band of order of U=1-2 eV. We discuss a possible effect of the latter electron correlation on the ferromagnetic instability of this compound.

  16. Magnetic Compton scattering study of CeRh3B2

    Science.gov (United States)

    Sakurai, Y.; Itou, M.; Tamura, J.; Nanao, S.; Thamizhavel, A.; Inada, Y.; Galatanu, A.; Yamamoto, E.; Onuki, Y.

    2003-07-01

    The spin-polarized electron momentum distribution (magnetic Compton profile: MCP) of ferromagnetic CeRh3B2 has been measured using the magnetic Compton scattering technique at 10 K for the [11bar 20] direction. In this compound, the orbital moment dominates the magnetization. An analysis of the MCP reveals that the Ce 4f and Ce 5d spin moments are aligned parallel with a value of -0.68 +/- 0.08 and 0.41 +/- 0.04muB, respectively, while Rh 4d electrons do not carry any significant spin moment. The ratio of orbital to spin moments for Ce 4f electrons, which has been determined from comparison of the present data with recent polarized neutron diffraction data, is rather smaller than that for Ce3+ ions. This result implies strong hybridization of the Ce 4f orbital with the orbitals on the surrounding ions.

  17. Inhibition of DYRK1A and GSK3B induces human β-cell proliferation

    Science.gov (United States)

    Shen, Weijun; Taylor, Brandon; Jin, Qihui; Nguyen-Tran, Van; Meeusen, Shelly; Zhang, You-Qing; Kamireddy, Anwesh; Swafford, Austin; Powers, Andrew F.; Walker, John; Lamb, John; Bursalaya, Badry; DiDonato, Michael; Harb, George; Qiu, Minhua; Filippi, Christophe M.; Deaton, Lisa; Turk, Carolina N.; Suarez-Pinzon, Wilma L.; Liu, Yahu; Hao, Xueshi; Mo, Tingting; Yan, Shanshan; Li, Jing; Herman, Ann E.; Hering, Bernhard J.; Wu, Tom; Martin Seidel, H.; McNamara, Peter; Glynne, Richard; Laffitte, Bryan

    2015-01-01

    Insufficient pancreatic β-cell mass or function results in diabetes mellitus. While significant progress has been made in regulating insulin secretion from β-cells in diabetic patients, no pharmacological agents have been described that increase β-cell replication in humans. Here we report aminopyrazine compounds that stimulate robust β-cell proliferation in adult primary islets, most likely as a result of combined inhibition of DYRK1A and GSK3B. Aminopyrazine-treated human islets retain functionality in vitro and after transplantation into diabetic mice. Oral dosing of these compounds in diabetic mice induces β-cell proliferation, increases β-cell mass and insulin content, and improves glycaemic control. Biochemical, genetic and cell biology data point to Dyrk1a as the key molecular target. This study supports the feasibility of treating diabetes with an oral therapy to restore β-cell mass, and highlights a tractable pathway for future drug discovery efforts. PMID:26496802

  18. Development of MMRPC prototype for the NeuLAND detector of the R3B collaboration

    Science.gov (United States)

    Pramanik, U. Datta; Chakraborty, S.; Basu, P.; Basu, J.; Banerjee, P.; Bemmerer, D.; Bose, S.; Chatterjee, S.; Elekes, Z.; Kempe, M.; Leifels, Y.; Panja, J.; Mukherjee, A.; Rahaman, A.; Roy, S.; Simon, H.; Sobiella, M.; Stach, D.; Wagner, A.; Yakorev, D.

    2012-01-01

    A prototype of Multi-strip Multi-gap Resistive Plate chamber (MMRPC) with active area 40cm×20cm has been developed at SINP, Kolkata as a new Time-Of-Flight (TOF) system with timing resolution σtEn>200MeV) TOF system for the R3B collaboration at the FAIR facility. The design of the detector elements is as follows: a double stack MMRPC with float glass plates and two gas gaps of 0.3 mm per stack. The response of this MMRPC has been studied with cosmic muons and γ-rays from a standard radioactive source (60Co) in coincidence with fast inorganic scintillators at SINP laboratory. Recently, response of developed MMRPC has been studied using pulsed electron beam at ELBE, FZD. The details of the MMRPC construction , experimental set-up for investigation of its response and first results are presented.

  19. [Pyrido[2,3-b][1,4]diazepinones with CNS activity].

    Science.gov (United States)

    Savelli, F; Boido, A; Pirisino, G; Piu, L; Satta, M; Manca, P

    1987-11-01

    In the interests of developing our research on compounds with a pyrazinone nucleus, cyclohomologues, characterised by the presence of one diazepinone nucleus, were prepared. The 5-[(dialkylamino)alkyl]-3,5-dihydro-2-methyl/phenyl-4H-pyrido[2,3- b][1,4]diazepin-4-ones obtained by means of condensation of the 2-(dialkylamino)alkylamino-3-aminopyridines with ethyl acetyl- or benzoyl- acetate, were subjected to pharmacological experimentation in order to evaluate their effect upon mice with regard to exploratory activity, motor coordination, and spontaneous activity. In addition their analgesic activity was evaluated and also their anti-strychnine, anti-cardiazole, anti-amphetamine and anti-reserpine activities.

  20. DNMT3B-283SNP与胃癌易感性的关联分析

    Institute of Scientific and Technical Information of China (English)

    刘东声

    2008-01-01

    目的:探讨DNMT3B基因-283T〉C单核苷酸多态性与胃癌易感性的关系.方法:提取外周血基因组DNA,采用PCR-RFLP结合DNA测序技术检测DNMT3B基因-283T〉C单核苷酸多态性.结果;携带DNMT3B TT等位基因的个体与对照组相比,其患胃癌的易感性明显升高(P=0.017,OR=1.46,95%CI:1.07~2.01).结论:DNMT3B基因-283T〉C多态性可能与胃癌的易感性有关联.

  1. Crystal structure and magnetization of a Co3B2O6 single crystal

    Science.gov (United States)

    Kazak, N. V.; Platunov, M. S.; Ivanova, N. B.; Knyazev, Yu. V.; Bezmaternykh, L. N.; Eremin, E. V.; Vasil'ev, A. D.; Bayukov, O. A.; Ovchinnikov, S. G.; Velikanov, D. A.; Zubavichus, Ya. V.

    2013-07-01

    The crystal structure and magnetic properties of Co3B2O6 single crystals are studied. Orthorhombic symmetry with space group Pnnm is detected at room temperature. The measurements of static magnetization and dynamic magnetic susceptibility reveal two magnetic anomalies at T 1 = 33 K and T 2 = 10 K and an easy-axis magnetic anisotropy. The effective magnetic moment indicates a high-spin state of the Co2+ ion. A spin-flop transition is found at low temperatures and H sf = 23 kOe. EXAFS spectra of the K-edge absorption of Co are recorded at various temperatures, the temperature-induced changes in the parameters of the local environment of cobalt are analyzed, and the effective Co-Co and Co-O distances are determined. The magnetic interactions in the crystal are analyzed in terms of an indirect coupling model.

  2. Metrics for NASA Aeronautics Research Mission Directorate (ARMD) Strategic Thrust 3B Vertical Lift Strategic Direction

    Science.gov (United States)

    Hochstetler, Ronald D.; Salvano, Dan; Gorton, Susan A.

    2017-01-01

    The NASA Aeronautics Research Mission Directorate (ARMD) Strategic Implementation Plan details an ambitious plan for aeronautical research for the next quarter century and beyond. It includes a number of advanced technologies needed to address requirements of the overall aviation community (domestic and international), with an emphasis on safety, efficiency, operational flexibility, and alternative propulsion air transport options. The six ARMD Strategic Thrust Areas (STAs) represent a specific set of multi-decade research agendas for creating the global aviation improvements most in demand by the aviation service consumers and the general public. To provide NASA with a measurement of the preeminent value of these research areas, it was necessary to identify and quantify the measurable benefits to the aviation community from capabilities delivered by the research programs. This paper will describe the processes used and the conclusions reached in defining the principal metrics for ARMD Strategic Thrust Area 3B "Vertical Lift Strategic Direction."

  3. Intermediate type excitons in Schottky barriers of A3B6 layer semiconductors and UV photodetectors

    Science.gov (United States)

    Alekperov, O. Z.; Guseinov, N. M.; Nadjafov, A. I.

    2006-09-01

    Photoelectric and photovoltaic spectra of Schottky barrier (SB) structures of InSe, GaSe and GaS layered semiconductors (LS) are investigated at quantum energies from the band edge excitons of corresponding materials up to 6.5eV. Spectral dependences of photoconductivity (PC) of photo resistors and barrier structures are strongly different at the quantum energies corresponding to the intermediate type excitons (ITE) observed in these semiconductors. It was suggested that high UV photoconductivity of A3B6 LS is due to existence of high mobility light carriers in the depth of the band structure. It is shown that SB of semitransparent Au-InSe is high sensitive photo detector in UV region of spectra.

  4. Pyrrolo[2,3-b]pyridine derivatives as potent Bruton's tyrosine kinase inhibitors.

    Science.gov (United States)

    Zhao, Xinge; Huang, Wei; Wang, Yazhou; Xin, Minhang; Jin, Qiu; Cai, Jianfeng; Tang, Feng; Zhao, Yong; Xiang, Hua

    2015-08-01

    A series of pyrrolo[2,3-b]pyridine-based derivatives were designed as potent Bruton's tyrosine kinase (BTK) inhibitors by using a scaffold-hopping strategy. Structure-activity relationship studies identified five compounds (3n, 3p, 3q, 3r, and 3s) with IC50 of less than 10nM in BTK enzyme assay and five compounds (3m, 3n, 3o, 3p, and 3t) with IC50 of less than 20 nM in Ramos cell assay. As one of the most potent inhibitors, compound 3p exhibited superior activity to that of compound 1 (RN486) and pyrrolo[2,3-d]pyrimidine derivative 2 in both BTK enzymatic (IC50=6.0 nM) and cellular inhibition (IC50=14 nM) assays. In addition, 3p displayed favorable overall pharmacokinetic profiles compared with 1 and 2.

  5. Molecular characterization of a KIF3B-like kinesin gene in the testis of Octopus tankahkeei (Cephalopoda, Octopus).

    Science.gov (United States)

    Dang, Ran; Zhu, Jun-Quan; Tan, Fu-Qing; Wang, Wei; Zhou, Hong; Yang, Wan-Xi

    2012-05-01

    KIF3B is known for maintaining and assembling cilia and flagellum. To date, the function of KIF3B and its relationship with KIF3A during spermiogenesis in the cephalopod Octopus tankahkeei remains unknown. In the present study, we characterized a gene encoding a homologue of rat KIF3B in the O. tankahkeei testis and examined its temporal and spatial expression pattern during spermiogenesis. The cDNA of KIF3B was obtained with degenerate and RACE PCR and the distribution pattern of ot-kif3b were observed with RT-PCR. The morphological development during spermiogenesis was illustrated by histological and transmission electron microscopy and mRNA expression of ot-kif3b was observed by in situ hybridization. The 2,365 nucleotides cDNA consisted of a 102 bp 5' untranslated region (UTR), a 2,208 bp open reading frame (ORF) encoding a protein of 736 amino acids, and a 55 bp 3' UTR. Multiple alignments revealed that the putative Ot-KIF3B shared 68, 68, 69, 68, and 67% identity with that of Homo sapiens, Mus musculus, Gallus gallus, Danio rerio, and Xenopus laevis, respectively, along with high identities with Ot-KIF3A in fundamental structures. Ot-kif3b transcripts appeared gradually in early spermatids, increased in intermediate spermatids and maximized in drastically remodeled and final spermatids. The kif3b gene is identified and its expression pattern is demonstrated for the first time in O. tankahkeei. Compared to ot-kif3a reported by our laboratory before, our data suggested that the putative heterodimeric motor proteins Ot-KIF3A/B may be involved in intraspermatic transport and might contribute to structural changes during spermiogenesis.

  6. Diurnal expression of Dnmt3b mRNA in mouse liver is regulated by feeding and hepatic clockwork.

    Science.gov (United States)

    Maekawa, Fumihiko; Shimba, Shigeki; Takumi, Shota; Sano, Tomoharu; Suzuki, Takehiro; Bao, Jinhua; Ohwada, Mika; Ehara, Tatsuya; Ogawa, Yoshihiro; Nohara, Keiko

    2012-09-01

    DNA methyltransferase 3B (DNMT3B) is critically involved in de novo DNA methylation and genomic stability, while the regulatory mechanism in liver is largely unknown. We previously reported that diurnal variation occurs in the mRNA expression of Dnmt3b in adult mouse liver. The aim of this study was to determine the mechanism underlying the diurnal expression pattern. The highest level and the lowest level of Dnmt3b mRNA expression were confirmed to occur at dawn and in the afternoon, respectively, and the expression pattern of Dnmt3b closely coincided with that of Bmal1. Since the diurnal pattern of Dnmt3b mRNA expression developed at weaning and scheduled feeding to separate the feeding cycle from the light/dark cycle led to a phase-shift in the expression, it could be assumed that feeding plays a critical role as an entrainment signal. In liver-specific Bmal1 knockout (L-Bmal1 KO) mice, L-Bmal1 deficiency resulted in significantly higher levels of Dnmt3b at all measured time points, and the time when the expression was the lowest in wild-type mice was shifted to earlier. Investigation of global DNA methylation revealed a temporal decrease of 5-methyl-cytosine percentage in the genome of wild-type mice in late afternoon. By contrast, no such decrease in 5-methyl-cytosine percentage was detected in L-Bmal1 KO mice, suggesting that altered Dnmt3b expression affects the DNA methylation state. Taken together, the results suggest that the feeding and hepatic clockwork generated by the clock genes, including Bmal1, regulate the diurnal variation in Dnmt3b mRNA expression and the consequent dynamic changes in global DNA methylation.

  7. Then and Now: Approaches to Understanding Children's Literature in Two Volumes

    Directory of Open Access Journals (Sweden)

    Baker M Bani-Khair

    2016-06-01

    Full Text Available This research paper investigates two main volumes taken from Children's Literature Association Quarterly; the earlier one is Vol. 11 published in 1986, and the other one, a more recent one, Vol. 32 published in 2007, as to understand the differences and similarities regarding the approaches used in the articles to understand Children's Literature in terms of its multifarious aspects such us, literary genres, styles, themes, and methodologies. Such kind of comparison yielded that those differences between those two volumes cover new thematic suggestions, new treatment and projection of characters, and strict scholarship which make the newer volume different in terms of approaches from the earlier one. The research also concluded that modern analytical perspectives are probably the most obvious transformations and changes in the second volume. Keywords: Volume, approach, childhood, construction, scholarship, transformation, modernism, historicism, analysis

  8. Study on the Retrieval of Snow Depth from FY3B/MWRI in the Atctic

    Science.gov (United States)

    Li, Lele; Chen, Haihua; Guan, Lei

    2016-06-01

    temperatures. Given the high albedo and low thermal conductivity, snow is regarded as one of the key reasons for the amplification of the warming in polar regions. The distributions of sea ice and snow depth are essential to the whole thermal conduction in the Arctic. This study focused on the retrieval of snow depth on sea ice from brightness temperatures of the MicroWave Radiometer Imager (MWRI) onboard the FengYun (FY)-3B satellite during the period from December 1, 2010 to April 30, 2011. After cross calibrated to the Advanced Microwave Scanning Radiometer-EOS (AMSR-E) Level 2A data, the MWRI brightness temperatures were applied to calculate the sea ice concentrations based on the Arctic Radiation and Turbulence Interaction Study Sea Ice (ASI) algorithm. According to the proportional relationship between the snow depth and the surface scattering in 18.7 and 36.5 GHz, the snow depths were derived. In order to eliminate the influence of uncertainties in grain sizes of snow as well as sporadic weather effects, the seven-day averaged snow depths were calculated. Then the results were compared with the snow depths from the AMSR-E Level 3 Sea Ice products. The bias of differences between the MWRI and the AMSR-E Level 3 products are ranged between -1.09 and -0.32 cm while the standard deviations and the correlation coefficients are ranged from 2.47 to 2.88 cm and from 0.78 to 0.90 for different months. As a result, it could be summarized that FY3B/MWRI showed a promising prospect in retrieving snow depth on sea ice.

  9. HATS-3b: An Inflated Hot Jupiter Transiting an F-type Star

    Science.gov (United States)

    Bayliss, D.; Zhou, G.; Penev, K.; Bakos, G. Á.; Hartman, J. D.; Jordán, A.; Mancini, L.; Mohler-Fischer, M.; Suc, V.; Rabus, M.; Béky, B.; Csubry, Z.; Buchhave, L.; Henning, T.; Nikolov, N.; Csák, B.; Brahm, R.; Espinoza, N.; Noyes, R. W.; Schmidt, B.; Conroy, P.; Wright, D. J.; Tinney, C. G.; Addison, B. C.; Sackett, P. D.; Sasselov, D. D.; Lázár, J.; Papp, I.; Sári, P.

    2013-11-01

    We report the discovery by the HATSouth survey of HATS-3b, a transiting extrasolar planet orbiting a V = 12.4 F dwarf star. HATS-3b has a period of P = 3.5479 days, mass of Mp = 1.07 M J, and radius of Rp = 1.38 R J. Given the radius of the planet, the brightness of the host star, and the stellar rotational velocity (vsin i = 9.0 km s-1), this system will make an interesting target for future observations to measure the Rossiter-McLaughlin effect and determine its spin-orbit alignment. We detail the low-/medium-resolution reconnaissance spectroscopy that we are now using to deal with large numbers of transiting planet candidates produced by the HATSouth survey. We show that this important step in discovering planets produces log g and T eff parameters at a precision suitable for efficient candidate vetting, as well as efficiently identifying stellar mass eclipsing binaries with radial velocity semi-amplitudes as low as 1 km s-1. The HATSouth network is operated by a collaboration consisting of Princeton University (PU), the Max Planck Institute für Astronomie (MPIA), and the Australian National University (ANU). The station at Las Campanas Observatory (LCO) of the Carnegie Institute is operated by PU in conjunction with collaborators at the Pontificia Universidad Católica de Chile (PUC), the station at the High Energy Spectroscopic Survey (HESS) site is operated in conjunction with MPIA, and the station at Siding Spring Observatory (SSO) is operated jointly with ANU.

  10. Anomalous ferromagnetism in CeRh3B2: Possibility of a new Kondo-lattice state

    Science.gov (United States)

    Shaheen, S. A.; Schilling, J. S.; Shelton, R. N.

    1985-01-01

    Information on the nature of the highly anomalous ferromagnetic state of CeRh3B2 below 115 K is gained by studying the evolution of the magnetic, superconducting, and structural properties across the quasiternary series LaxCe1-xRh3B2 and Ce(RuyRh1-y)3B2. The present results offer considerable evidence that this ferromagnetism originates from the ordering of Ce local moments and not, as has been claimed, from itinerant magnetism in the Rh 4d band.

  11. "BION-M" No. 1 spacecraft radiation environment as observed by the RD3-B3 radiometer-dosimeter in April-May 2013

    Science.gov (United States)

    Dachev, T. P.; Tomov, B. T.; Matviichuk, Yu. N.; Dimitrov, Pl. G.; Bankov, N. G.; Shurshakov, V. V.; Ivanova, O. A.; Häder, D.-P.; Schuster, M. T.; Reitz, G.; Horneck, G.

    2015-02-01

    Space radiation has been monitored using the РД3-Б3 (in the following we use the Latin transcription RD3-B3) spectrometer-dosimeter on board a recent space flight of the Russian recoverable satellite "BION-M" No. 1. The instrument was mounted inside the satellite in a pressurized volume together with biological objects and samples. The RD3-B3 instrument is a battery operated version of the spare model of the R3D-B3 instrument developed and built for the ESA BIOPAN-6 facility on Foton M3 satellite launched on September 2007 (Häder et al., 2009). It is a low mass, small dimension automated device that measures solar radiation in four channels and ionizing radiation in 256 channels of a Liulin-type energy deposition spectrometer (Dachev et al., 2002). Cosmic ionizing radiation has been monitored and separated in 256 deposited energy spectra, which were further used for determination of the absorbed dose rate and flux. The paper summarizes the results for the Earth radiation environment at the altitude of 253-585 km.

  12. Two-sided bounds for the volume of right-angled hyperbolic polyhedra

    CERN Document Server

    Repovš, Dušan

    2011-01-01

    For a compact right-angled polyhedron $R$ in $\\mathbb H^3$ denote by $\\operatorname{vol} (R)$ the volume and by $\\operatorname{vert} (R)$ the number of vertices. Upper and lower bounds for $\\operatorname{vol} (R)$ in terms of $\\operatorname{vert} (R)$ were obtained in \\cite{A09}. Constructing a 2-parameter family of polyhedra, we show that the asymptotic upper bound $5 v_3 / 8$, where $v_3$ is the volume of the ideal regular tetrahedron in $\\mathbb H^3$, is a double limit point for ratios $\\operatorname{vol} (R) / \\operatorname{vert} (R)$. Moreover, we improve the lower bound in the case $\\operatorname{vert} (R) \\leqslant 56$.

  13. Editor's welcome, PORTAL, Vol. 1, No. 2, July 2004

    Directory of Open Access Journals (Sweden)

    Paul Allatson

    2005-03-01

    Full Text Available Since the highly successful inauguration of PORTAL in January 2004, we have received many kind expressions of support from international studies practitioners in a range of fields, and from such places as Canada, Italy, Mexico, Nigeria, New Zealand, Spain, Trinidad, the U.K., and the U.S.A. Particularly gratifying have been the endorsements of the journal and its publishing aims by people involved in their own electronic publishing enterprises. For their generous responses to PORTAL, the Editorial Committee would like to express its collective appreciation to the following people: Professor Jean-Marie Volet, of the University of Western Australia, and the guiding editor of the ground-breaking e-journal Mots Pluriels (www.arts.uwa.edu.au/MotsPluriels; and Francis Leo Collins, member of the Editorial Committee for the Graduate Journal of Asia Pacific Studies (GJAPS, based in Auckland, New Zealand. PORTAL's readers may be interested in the current call for papers from GJAPS (www.arts.auckland.ac.nz/gjaps, for a special issue on "Imagining the Asia-Pacific" (deadline October 31, 2004. This issue of PORTAL contains essays that cover wide terrain: the Chilean diasporic community in Australia; the world of German intellectuals; contemporary Mexican socio-political movements; rural-urban migration in China; and transnational advocacy networks and election monitoring in the Philippines, Chile, Nicaragua and Mexico. In the cultural section of this issue, we are delighted to present a short story from the noted German Studies scholar Anthony Stephens, and the first half of a beautiful, deeply poetic and haunting novel entitled Son, from the London-based writer and art-critic Jennifer Higgie. The novel’s second and final part will appear in PORTAL vol. 2, no. 1, in January 2005. On a different note, we would like to express our support for the inaugural Ubud Writers and Readers Festival, to be held in Ubud, Bali, from October 11 to 17, 2004. The Festival

  14. Quantitative analysis of photoquadrats acquired at Johnston Atoll permanent transect site 3B-P on July 1, 2000

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This spreadsheet summarizes the number of corals photographed along a permanent transect line at Underwater Site 3B-P at Johnston Atoll on 7/1/2000.

  15. Regioselective synthesis of 3-arylpyrido[2,3-b]pyrazines by reaction of arylglyoxals with 2,3-diaminopyridine

    Directory of Open Access Journals (Sweden)

    Jabbar Khalafy

    2013-01-01

    Full Text Available A series of pyrido[2,3-b]pyrazine derivatives were synthesized in good to excellent yields by condensation reactions of arylglyoxals with 2,3-diaminopyridine in dimethylformamide and ethanol at 90 oC.

  16. A DNA methylation signature associated with aberrant promoter DNA hypermethylation of DNMT3B in human colorectal cancer.

    Science.gov (United States)

    Huidobro, Covadonga; Urdinguio, Rocío G; Rodríguez, Ramón María; Mangas, Cristina; Calvanese, Vincenzo; Martínez-Camblor, Pablo; Ferrero, Cecilia; Parra-Blanco, Adolfo; Rodrigo, Luis; Obaya, Alvaro J; Suárez-Fernández, Laura; Astudillo, Aurora; Hernando, Henar; Ballestar, Esteban; Fernández, Agustín F; Fraga, Mario F

    2012-09-01

    Altered promoter DNA methylation, one of the most important molecular alterations in cancer, is proposed to correlate with deregulation of DNA methyltransferases, although the molecular mechanisms implicated are still poorly understood. Here we show that the de novo DNA methyltransferase DNMT3B is frequently repressed in human colorectal cancer cell lines (CCL) and primary tumours by aberrant DNA hypermethylation of its distal promoter. At the epigenome level, DNMT3B promoter hypermethylation was associated with the hypomethylation of gene promoters usually hypermethylated in the healthy colon. Forced DNMT3B overexpression in cancer cells restored the methylation levels of these promoters in the healthy colon. Our results show a new molecular mechanism of aberrant DNMT3B regulation in colon cancer and suggest that its expression is associated with the methylation of constitutively hypermethylated promoters in the healthy colon. Copyright © 2011 Elsevier Ltd. All rights reserved.

  17. The admissibility criterion under article 35(3)(b) ECHR: a "significant disadvantage" to human rights protection? / Nikos Vogiatzis

    Index Scriptorium Estoniae

    Vogiatzis, Nikos

    2016-01-01

    Vastuvõetavuse kriteeriumist Inimõiguste ja põhivabaduste kaitse konventsiooni artikli 35(3)(b) alusel, artiklist tulenevast piirangust õigusemõistmisele rahvusvahelisel tasandil ja individuaalkaebuse esitamise piirangutest Euroopa Inimõiguste Kohtusse

  18. Weak Ferromagnetism below 41 K and Structural Transition at 395 K in CeIr3B2 Single Crystal

    Science.gov (United States)

    Kubota, Kazuhiro; Matsuoka, Eiichi; Funasako, Yusuke; Mochida, Tomoyuki; Sakurai, Takahiro; Ohta, Hitoshi; Onimaru, Takahiro; Takabatake, Toshiro; Sugawara, Hitoshi

    2013-10-01

    We report on the discovery of ferromagnetic behavior below TC = 41 K in CeIr3B2 single crystals by means of magnetization, electrical resistivity, and specific heat measurements. The ferromagnetic transition temperature is unusually high despite the lack of magnetic elements except Ce, which is the second highest transition temperature recorded after CeRh3B2 (TC˜ 120 K). The ferromagnetic ordered moment is estimated to be μs˜ 0.04 μB/Ce at 1.8 K, which lies in the c-plane of a monoclinic crystal structure. A structural phase transition from monoclinic to hexagonal occurs approximately at 395 K upon heating. The similarity and difference in physical properties between CeIr3B2 and CeRh3B2 are discussed.

  19. The admissibility criterion under article 35(3)(b) ECHR: a "significant disadvantage" to human rights protection? / Nikos Vogiatzis

    Index Scriptorium Estoniae

    Vogiatzis, Nikos

    2016-01-01

    Vastuvõetavuse kriteeriumist Inimõiguste ja põhivabaduste konventsiooni artikli 35(3)(b) alusel, artiklist tulenevast piirangust õigusemõistmisele rahvusvahelisel tasandil ja individuaalkaebuse esitamise piirangutest Euroopa Inimõiguste Kohtusse

  20. TiO2/beads as a photocatalyst for the degradation of X3B azo dye

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    The feasibility of photocatalytic degradation of X3B azo dye by TiO2/beads photocatalyst was studied. The effects of parameters such as the amount of TiO2/beads, airflow, as well as the concentrations of H2O2, Fe3+, Mg2+ and Na+ on the photocatalytic degradation of X3B azo dye were also studied. The results showed that 25 mg/dm3 X3B azo dye can be photocatalytically degraded completely by 30 min illumination with a 375W medium pressure mercury lamp. Adding a small amount of H2O2 or Fe3+, the efficiencies of photocatalytic degradation of X3B azo dye were increased rapidly. The mechanisms of the reaction and the role of the additives were also investigated. After 120 hours TiO2/beads showed no significant loss of the photocatalytic activity.

  1. Streptococcus pyogenes Employs Strain-dependent Mechanisms of C3b Inactivation to Inhibit Phagocytosis and Killing of Bacteria.

    Science.gov (United States)

    Agrahari, Garima; Liang, Zhong; Glinton, Kristofor; Lee, Shaun W; Ploplis, Victoria A; Castellino, Francis J

    2016-04-22

    Evasion of complement-mediated opsonophagocytosis enables group A Streptococcus pyogenes (GAS) to establish infection. Different strain-dependent mechanisms are employed by the host to accomplish this goal. In general, GAS inhibits the amplification of the complement cascade on its cell surface by facilitating the degradation of C3b, an opsonin, to an inactive product, inactivated C3b (iC3b), in a step catalyzed by factor I (FI) and its cofactor, factor H (FH), with or without the participation of human host plasmin (hPm). GAS recruits FH to its cell surface via FH receptors, which are transcriptionally controlled by the two-component cluster of virulence responder-sensor system. The manner in which FI-FH and hPm function together on GAS cells is unknown. Using GAS strain AP53, which strongly binds host human plasminogen/plasmin (hPg/hPm) directly via an hPg/hPm surface receptor (PAM), we show that both FI-FH and hPm sequentially cleave C3b. Whereas FI-FH proteolytically cleaves C3b into iC3b, PAM-bound hPm catalyzes cleavage of iC3b into multiple smaller peptides. Unlike AP53, GAS strain M23ND weakly binds FH and recruits hPg/hPm to its cell surface indirectly via fibrinogen bound to M-protein, M23. In this case, FH-FI cleaves C3b into iC3b, with negligible degradation of iC3b by hPm that is bound to fibrinogen on the cells. AP53 and M23ND display similar resistance to human neutrophil-mediated phagocytosis, which results in a corresponding high lethality in mice after injection of these cells. These results suggest that GAS utilizes diverse mechanisms to degrade C3b and thus to protect bacterial cells from the complement response of the host.

  2. Well data summary sheets. Vol. 33: North sea wells

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-12-31

    This 33rd volume of the `Well Data Summary sheets` includes data from seven recently released offshore wells from the North Sea. The wells Baron-2, S.E. Adda-1 and Skjold Flank-1 were presented in our `Well Data Summary Sheets, special volume`, published May 1, 1997. In volume 33, the wells Baron-2 and Skjold Flank-1 are updated with respect to information on sidewall cores. Information regarding all released well data, included in our well data summary sheets, are available on GEUS homepage: http://www.geus.dk/ or the departments homepage: http://www.geus.dk/departments/geol-info-data-centre/geoldata.htm/. A complete index of releases wells in volumes 17-33 is included. (au)

  3. FILOSOFIA THEORETICA FORMATED Vol 2 No. 1. Final 1docx

    African Journals Online (AJOL)

    JONATHAN

    The issues concerning African studies addressed in this volume are quite diverse and ... and promote a new phase of African philosophy, culture, history and religion .... by J. O. Chimakonam entitled Introducing African Science: Systematic and ...

  4. Palladium-Catalyzed Tandem Carbene Migratory Insertion and Intramolecular Cyclization: Synthesis of Chromeno[4,3-b]chromene Compounds.

    Science.gov (United States)

    Shang, Xue Song; Li, Nian Tai; Siyang, Hai Xiao; Liu, Pei Nian

    2015-05-01

    Chromeno[4,3-b]chromene is a ubiquitous structural motif found in various pharmaceuticals and biologically active compounds. A concise palladium-catalyzed reaction of vinyl iodides and salicyl N-tosylhydrazones has been achieved to afford a series of compounds containing the chromeno[4,3-b]chromene scaffold in moderate to high yield. This tandem reaction involves palladium(II) carbene migratory insertion and intramolecular cyclization assisted by an O nucleophile and tolerates various functional groups.

  5. Distinct recognition of complement iC3b by integrins αXβ2 and αMβ2.

    Science.gov (United States)

    Xu, Shutong; Wang, Jianchuan; Wang, Jia-Huai; Springer, Timothy A

    2017-03-28

    Recognition by the leukocyte integrins αXβ2 and αMβ2 of complement iC3b-opsonized targets is essential for effector functions including phagocytosis. The integrin-binding sites on iC3b remain incompletely characterized. Here, we describe negative-stain electron microscopy and biochemical studies of αXβ2 and αMβ2 in complex with iC3b. Despite high homology, the two integrins bind iC3b at multiple distinct sites. αXβ2 uses the αX αI domain to bind iC3b on its C3c moiety at one of two sites: a major site at the interface between macroglobulin (MG) 3 and MG4 domains, and a less frequently used site near the C345C domain. In contrast, αMβ2 uses its αI domain to bind iC3b at the thioester domain and simultaneously interacts through a region near the αM β-propeller and β2 βI domain with a region of the C3c moiety near the C345C domain. Remarkably, there is no overlap between the primary binding site of αXβ2 and the binding site of αMβ2 on iC3b. Distinctive binding sites on iC3b by integrins αXβ2 and αMβ2 may be biologically beneficial for leukocytes to more efficiently capture opsonized pathogens and to avoid subversion by pathogen factors.

  6. Promoter polymorphisms of DNMT3B and the risk of colorectal cancer in Chinese: a case-control study

    Directory of Open Access Journals (Sweden)

    Liu Dongsheng

    2008-07-01

    Full Text Available Abstract Background DNA-methyltransferase-3B (DNMT3B, which plays a role in DNA methylation, is usually aberrant expression involved in carcinogenesis. Polymorphisms of the DNMT3B gene may influence DNMT3B activity on DNA methylation in several cancers, thereby modulating the susceptibility to cancer. Methods DNMT3B -579G>T genotypes and -149C>T were determined by PCR-RFLP and sequencing in 137 colorectal cancer patients and 308 controls matched for age and sex, who did not receive radiotherapy or chemotherapy for newly diagnosed and histopathologically confirmed colorectal cancer. The association between two SNPs of the DNMT3B promoter and the risk of the development of colorectal cancer was analyzed in a population of Chinese. Results The allele frequency of -149C >T among patients and controls was 0.73% versus 0.65%, respectively. The allele frequency of -597G>T for patients and controls was 6.57% versus 11.53%, respectively. Individuals with at least one -149C>T allele were no at a significantly increase risk of colorectal cancer compared with those having a -149TT genotype. However, Individuals with at least one 579G>T allele were decreased risk of colorectal cancer compared with those having a -579TT genotype. Conclusion The relative distribution of -149C>T DNMT3B SNPs among a Chinese population can not be used as a stratification marker to predict an individual's susceptibility to colorectal cancer. However, the DNMT3B -579G>T polymorphism may contribute to the genetic susceptibility to colorectal cancer.

  7. In vivo redundant function of the 3' IgH regulatory element HS3b in the mouse.

    Science.gov (United States)

    Bébin, Anne-Gaëlle; Carrion, Claire; Marquet, Marie; Cogné, Nadine; Lecardeur, Sandrine; Cogné, Michel; Pinaud, Eric

    2010-04-01

    In the mouse, the regulatory region located at the 3' end of the IgH locus includes four transcriptional enhancers: HS3a, HS1-2, HS3b, and HS4; the first three lie in a quasi-palindromic structure. Although the upstream elements HS3a and HS1-2 proved dispensable for Ig expression and class switch recombination (CSR), the joint deletion of HS3b and HS4 led to a consistent decrease in IgH expression in resting B cells and to a major CSR defect. Within this pair of distal enhancers, it was questionable whether HS3b and HS4 could be considered individually as elements critical for IgH expression and/or CSR. Studies in HS4-deficient mice recently revealed the role of HS4 as restricted to Igmicro-chain expression from the pre-B to the mature B cell stage and left HS3b as the last candidate for CSR regulation. Our present study finally invalidates the hypothesis that CSR could mostly rely on HS3b itself. B cells from HS3b-deficient animals undergo normal proliferation, germline transcription, and CSR upon in vitro stimulation with LPS; in vivo Ag-specific responses are not affected. In conclusion, our study highlights a major effect of the global ambiance of the IgH locus; enhancers demonstrated as being strongly synergistic in transgenes turn out to be redundant in their endogenous context.

  8. 6H-Indolo[2,3-b]quinoxalines: DNA and protein interacting scaffold for pharmacological activities.

    Science.gov (United States)

    Moorthy, N S Hari Narayana; Manivannan, E; Karthikeyan, C; Trivedi, Piyush

    2013-08-01

    6H-Indolo[2,3-b]quinoxaline, a planar fused heterocyclic compound exhibits a wide variety of pharmacological activities. The mechanism of pharmacological action exerted by these compounds is predominantly DNA intercalation. The thermal stability of the intercalated complex (DNA and 6H-indolo[2,3-b]quinoxaline derivatives) is an important parameter for the elucidation of anticancer, antiviral and other activities. This thermal stability of the 6H-indolo[2,3- b]quinoxaline-DNA complex depends on the type of substituents and side chains attached to the 6H-indolo[2,3- b]quinoxaline nucleus and also the orientation of the side chain towards the GC rich minor groove of the DNA. Highly active 6H-indolo[2,3-b]quinoxaline derivatives such as NCA0424, B-220 and 9-OH-B-220 have shown good binding affinity to DNA as evident from high thermal stability of compound-DNA complex. Interestingly, these compounds possessed poor inhibitory activity on topoisomerase II enzyme but have significant MDR modulating activity. This review establishes '6H-indolo[2,3-b]quinoxaline' as a valuable template for design and development of novel molecules with different biological activities.

  9. Heterocyst-specific flavodiiron protein Flv3B enables oxic diazotrophic growth of the filamentous cyanobacterium Anabaena sp. PCC 7120.

    Science.gov (United States)

    Ermakova, Maria; Battchikova, Natalia; Richaud, Pierre; Leino, Hannu; Kosourov, Sergey; Isojärvi, Janne; Peltier, Gilles; Flores, Enrique; Cournac, Laurent; Allahverdiyeva, Yagut; Aro, Eva-Mari

    2014-07-29

    Flavodiiron proteins are known to have crucial and specific roles in photoprotection of photosystems I and II in cyanobacteria. The filamentous, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120 contains, besides the four flavodiiron proteins Flv1A, Flv2, Flv3A, and Flv4 present in vegetative cells, two heterocyst-specific flavodiiron proteins, Flv1B and Flv3B. Here, we demonstrate that Flv3B is responsible for light-induced O2 uptake in heterocysts, and that the absence of the Flv3B protein severely compromises the growth of filaments in oxic, but not in microoxic, conditions. It is further demonstrated that Flv3B-mediated photosynthetic O2 uptake has a distinct role in heterocysts which cannot be substituted by respiratory O2 uptake in the protection of nitrogenase from oxidative damage and, thus, in an efficient provision of nitrogen to filaments. In line with this conclusion, the Δflv3B strain has reduced amounts of nitrogenase NifHDK subunits and shows multiple symptoms of nitrogen deficiency in the filaments. The apparent imbalance of cytosolic redox state in Δflv3B heterocysts also has a pronounced influence on the amounts of different transcripts and proteins. Therefore, an O2-related mechanism for control of gene expression is suggested to take place in heterocysts.

  10. Splicing factor SF3B1 mutations and ring sideroblasts in myelodysplastic syndromes: a Brazilian cohort screening study

    Directory of Open Access Journals (Sweden)

    Flávia Sacilotto Donaires

    Full Text Available ABSTRACT Background: Myelodysplastic syndromes (MDS comprise a group of malignant clonal hematologic disorders characterized by ineffective hematopoiesis and propensity for progression to acute myeloid leukemia. Acquired mutations in the gene encoding RNA splicing factor 3B subunit 1 (SF3B1 are highly associated with the MDS subtypes presenting ring sideroblasts, and represent a specific nosological entity. The effects of these mutations on clinical outcomes are diverse and contrasting. Methods: A cohort of 91 Brazilian MDS patients, including patients with ring sideroblasts in the bone marrow, were screened for mutations in the SF3B1 hotspots (exons 12-15 by direct Sanger sequencing. Results: SF3B1 heterozygous mutations were identified in six patients (7%, all of them with ring sideroblasts, thus confirming the association between SF3B1 mutations and myelodysplastic syndrome subtypes bearing this morphologic feature (frequency of 6/13, p-value < 0.0001. Conclusion: This is the first screening of SF3B1 mutations in a cohort of Brazilian myelodysplastic syndrome patients. Our findings confirm that mutations in this splicing gene correlate with bone marrow ringed sideroblasts.

  11. ICF syndrome mutations cause a broad spectrum of biochemical defects in DNMT3B-mediated de novo DNA methylation.

    Science.gov (United States)

    Moarefi, Amir H; Chédin, Frédéric

    2011-06-24

    The DNMT3B de novo DNA methyltransferase (DNMT) plays a major role in establishing DNA methylation patterns in early mammalian development, but its catalytic mechanism remains poorly characterized. Here, we provide a comprehensive biochemical analysis of human DNMT3B function through the characterization of a series of site-directed DNMT3B variants associated with immunodeficiency, centromere instability, and facial anomalies (ICF) syndrome. Our data reveal several novel and important aspects of DNMT3B function. First, DNMT3B, unlike DNMT3A, requires a DNA cofactor in order to stably bind to S-adenosyl-l-methionine (SAM), suggesting that it proceeds according to an ordered catalytic scheme. Second, ICF mutations cause a broad spectrum of biochemical defects in DNMT3B function, including defects in homo-oligomerization, SAM binding, SAM utilization, and DNA binding. Third, all tested ICF mutations, including the A766P and R840Q variants, result in altered catalytic properties without interfering with DNMT3L-mediated stimulation; this indicates that DNMT3L is not involved in the pathogenesis of ICF syndrome. Finally, our study reveals a novel level of coupling between substrate binding, oligomerization, and catalysis that is likely conserved within the DNMT3 family of enzymes.

  12. Hybrid organic – silica nanomaterials based on novel A{sub 3}B mixed substituted porphyrin

    Energy Technology Data Exchange (ETDEWEB)

    Fagadar-Cosma, Eugenia [Institute of Chemistry -Timisoara of Romanian Academy, M. Viteazul Ave, No. 24, 300223 Timisoara (Romania); Dudás, Zoltán, E-mail: dudas.zoltan@wigner.mta.hu [Institute of Chemistry -Timisoara of Romanian Academy, M. Viteazul Ave, No. 24, 300223 Timisoara (Romania); MTA Wigner Research Center for Physics, Konkoly Thege Miklós Street, No. 29-33, 1121 Budapest (Hungary); Birdeanu, Mihaela [Institute of Chemistry -Timisoara of Romanian Academy, M. Viteazul Ave, No. 24, 300223 Timisoara (Romania); National Institute for Research and Development in Electrochemistry and Condensed Matter, 1 Plautius Andronescu Street, 300224 Timisoara (Romania); Almásy, László [MTA Wigner Research Center for Physics, Konkoly Thege Miklós Street, No. 29-33, 1121 Budapest (Hungary)

    2014-11-14

    A new A{sub 3}B porphyrin structure, namely: 5-(4-phenoxyphenyl)-10,15,20-tris(4-pyridyl)-porphyrin was synthetized and characterized by FT-IR, UV–vis, Fluorescence, MS, {sup 1}H NMR, TLC and HPLC. Novel hybrid-silica porphyrin nanomaterials were obtained by immobilizing the porphyrin in silica supports synthesized from tetraethoxysilane, tetramethoxysilane or mixtures of tetraethoxysilane/methyltriethoxysilane. Since the behavior and performance of immobilized porphyrin molecules in the silica matrices strongly depend on the structure of the porous network, a comparative characterization of the silica support and the hybrid porphyrin-silica materials was carried out using specific physicochemical characterization methods: UV–vis, Fluorencence, FT-IR spectroscopy, thermal analysis, AFM, nitrogen adsorption and small-angle neutron scattering. The UV–vis spectra show that no protonation and aggregation of porphyrin takes place in the gels made from methyltriethoxysilane precursor. Most of the emission spectra preserve both the shape and the intensity of the corresponding free porphyrin. Due to the lack of aggregation, when using the methyltriethoxysilane precursor, the quenching of fluorescence is also diminished. No matter of the preparation method the specific surface areas increase in the following order: TEOS < TMOS < TEOS/MTES 3:1 < TEOS/MTES 2:1 < TEOS/MTES 1:1. Due to their optical properties, both the novel porphyrin and its derived hybrid materials, especially those synthesized in situ with mixtures of silica precursors TEOS/MTES will be sent for further medical trials in PDT, having characteristics of second generation photosensitizers. Due to large specific surface areas, the same materials will be used as sensitive materials in microsensors for air quality control, to detect the presence of CO, NO{sub x}, excess of CO{sub 2} and low level of O{sub 2}. - Highlights: • Synthesis of new A{sub 3}B type porphyrin exhibiting high fluorescence

  13. Bacillus anthracis interacts with plasmin(ogen to evade C3b-dependent innate immunity.

    Directory of Open Access Journals (Sweden)

    Myung-Chul Chung

    Full Text Available The causative agent of anthrax, Bacillus anthracis, is capable of circumventing the humoral and innate immune defense of the host and modulating the blood chemistry in circulation to initiate a productive infection. It has been shown that the pathogen employs a number of strategies against immune cells using secreted pathogenic factors such as toxins. However, interference of B. anthracis with the innate immune system through specific interaction of the spore surface with host proteins such as the complement system has heretofore attracted little attention. In order to assess the mechanisms by which B. anthracis evades the defense system, we employed a proteomic analysis to identify human serum proteins interacting with B. anthracis spores, and found that plasminogen (PLG is a major surface-bound protein. PLG efficiently bound to spores in a lysine- and exosporium-dependent manner. We identified α-enolase and elongation factor tu as PLG receptors. PLG-bound spores were capable of exhibiting anti-opsonic properties by cleaving C3b molecules in vitro and in rabbit bronchoalveolar lavage fluid, resulting in a decrease in macrophage phagocytosis. Our findings represent a step forward in understanding the mechanisms involved in the evasion of innate immunity by B. anthracis through recruitment of PLG resulting in the enhancement of anti-complement and anti-opsonization properties of the pathogen.

  14. Preparation of Fly ash Based Adsorbents for Removal Active Red X-3B from Dying Wastewater

    Directory of Open Access Journals (Sweden)

    Li Jinping

    2016-01-01

    Full Text Available Fly ash with a large number of active sites can occur with the adsorbent chemical and physical adsorption, and therefore have a strong adsorption capacity. The original fly ash and raw fly ash compared to the physical and chemical properties to a significant change. On the fly ash in industrial water treatment application were outlined. The purpose is to focus on the modification methods of fly ash and comparison of raw fly ash and fly ash in the effect of dyeing wastewater. Single factor test method; select the appropriate modifier to study the dosage, pH, stirring time on the modification of adsorption properties of fly ash before and after. The results showed that the modified fly ash was better than the adsorption. Greatly improves on active red X-3B dye wastewater removal capacity, pH = 5, 6, dosage is 5g / L, the mixing time is 30min, COD removal rate reached 73.07%. This modified material can be used as adsorbent for pre-treating dying wastewater.

  15. Isolation and purification of Cu-free methanobactin from Methylosinus trichosporium OB3b

    Directory of Open Access Journals (Sweden)

    Kraemer Stephan M

    2011-02-01

    Full Text Available Abstract Background The isolation of highly pure copper-free methanobactin is a prerequisite for the investigation of the biogeochemical functions of this chalkophore molecule produced by methane oxidizing bacteria. Here, we report a purification method for methanobactin from Methylosinus trichosporium OB3b cultures based on reversed-phase HPLC fractionation used in combination with a previously reported resin extraction. HPLC eluent fractions of the resin extracted product were collected and characterized with UV-vis, FT-IR, and C-1s NEXAFS spectroscopy, as well as with elemental analysis and ESI-MS. Results The results showed that numerous compounds other than methanobactin were present in the isolate obtained with resin extraction. Molar C/N ratios, mass spectrometry measurements, and UV-vis spectra indicated that methanobactin was only present in one of the HPLC fractions. On a mass basis, methanobactin carbon contributed only 32% to the total organic carbon isolated with resin extraction. Our spectroscopic results implied that besides methanobactin, the organic compounds in the resin extract comprised breakdown products of methanobactin as well as polysaccharide-like substances. Conclusion Our results demonstrate that a purification step is indispensable in addition to resin extraction in order to obtain pure methanobactin. The proposed HPLC purification procedure is suitable for semi-preparative work and provides copper-free methanobactin.

  16. Lupus-TR-3b: A Low-Mass Transiting Hot Jupiter in the Galactic Plane?

    CERN Document Server

    Weldrake, David T F; Sackett, Penny D; Tingley, Brandon W; Gillon, Michael; Setiawan, Johny

    2007-01-01

    We present a prime case for a transiting Hot Jupiter planet identified during a single-field transit survey towards the Lupus region of the Galactic plane. The object, Lupus-TR-3b, transits a V=17.4 K1V host star every 3.91405d. Spectroscopy and stellar colors indicate a host star with effective temperature 5000+/-150 K, with a stellar mass and radius of 0.87+/-0.04 Msun and 0.82+/-0.05 Rsun, respectively. Limb-darkened transit fitting yields a companion radius of 0.89+/-0.07 Rjup and an orbital inclination of 88.3{+1.3}{-0.8} deg. Magellan 6.5m MIKE radial velocity measurements reveal a 2.4 sigma K=114+/-25 m/s sinusoidal variation in phase with the transit ephemeris. The resulting mass is 0.81+/-0.18 Mjup and density 1.4+/-0.4 g/cm^3. Y-band PANIC image deconvolution reveal a V>=21 red neighbor 0.4'' away which, although highly unlikely, we cannot conclusively rule out as a blended binary with current data. However, no in-phase bisector variations are observed and blend simulations show that only the most u...

  17. Correction of TRMM 3B42V7 Based on Linear Regression Models over China

    Directory of Open Access Journals (Sweden)

    Shaohua Liu

    2016-01-01

    Full Text Available High temporal-spatial precipitation is necessary for hydrological simulation and water resource management, and remotely sensed precipitation products (RSPPs play a key role in supporting high temporal-spatial precipitation, especially in sparse gauge regions. TRMM 3B42V7 data (TRMM precipitation is an essential RSPP outperforming other RSPPs. Yet the utilization of TRMM precipitation is still limited by the inaccuracy and low spatial resolution at regional scale. In this paper, linear regression models (LRMs have been constructed to correct and downscale the TRMM precipitation based on the gauge precipitation at 2257 stations over China from 1998 to 2013. Then, the corrected TRMM precipitation was validated by gauge precipitation at 839 out of 2257 stations in 2014 at station and grid scales. The results show that both monthly and annual LRMs have obviously improved the accuracy of corrected TRMM precipitation with acceptable error, and monthly LRM performs slightly better than annual LRM in Mideastern China. Although the performance of corrected TRMM precipitation from the LRMs has been increased in Northwest China and Tibetan plateau, the error of corrected TRMM precipitation is still significant due to the large deviation between TRMM precipitation and low-density gauge precipitation.

  18. Ferromagnetism and crystal electric field in the cerium compound CeRh3B2

    Science.gov (United States)

    Givord, F.; Boucherle, J.-X.; Galéra, R.-M.; Fillion, G.; Lejay, P.

    2007-09-01

    The magnetic behavior of CeRh3B2 is very unusual: it orders ferromagnetically with an exceptionally high Curie temperature TC around 115 K, but with a small saturation moment of about 0.4 μB/fu. The thermal variations of magnetization and susceptibility have been measured on a single crystal for fields applied along the easy and hard magnetization directions, with, in the latter case, special care taken to avoid rotation of the sample. The results are compared to calculations based on the crystalline electric field formalism. Due to its large value, one has to take into account the admixture of the two J multiplets of the Ce3+ ion as it is usually used for samarium compounds but not for cerium ones. These calculations actually show the important role of the excited J' = 7/2 multiplet on the 4f spin moment, leading to an enhancement of the exchange, i.e. the Curie temperature, and to a decrease of the moment. An extra polarization, as previously found from the study of magnetization density maps, is confirmed and discussed.

  19. Magnetic properties of CeRh3B2 investigated by muon spin rotation spectroscopy

    Science.gov (United States)

    Gygax, F. N.; Schenck, A.; Ōnuki, Y.; Reichl, Ch.; Wiesinger, G.

    2006-04-01

    We report on positive muon spin rotation (μ+SR) measurements on hexagonal CeRh3B2 single crystals. This ferromagnetic material, with the high Curie temperature of TC≈120K , shows anomalous magnetic properties. In the magnetically ordered state, two spontaneous μ+ -precession frequencies are observed in zero applied magnetic field. The peculiar temperature dependence of these frequencies shows that a magnetization is found on Ce and, over a wide temperature range, on the Rh atoms. There is a strong indication that a significant negative fraction of the magnetization connected to the Ce atoms is delocalized on the c -axis Ce-Ce chains. The analysis renders it necessary to allow for an unusual temperature dependence of the Rh-sublattice magnetization, deviating significantly from the Ce-sublattice magnetization. This points to a temperature dependent exchange coupling between the Ce and Rh 4f and 4d states. Transverse-field measurements above TC allow one to specify the interstitial sites occupied by the muons and indicate the presence of long-range μ+ diffusion for T>125K .

  20. Synthesis, characterization and computational studies of a novel thieno[2,3-b]thiophene derivative

    Science.gov (United States)

    Mabkhot, Yahia N.; Barakat, Assem; Soliman, Saied M.; El-Idreesy, Tamer T.; Ghabbour, Hazem A.; Al-Showiman, Salim S.

    2017-02-01

    Thieno[2,3-b]thiophene incorporating enaminone (4) was synthesized and characterized by various spectroscopic tools such as 1H NMR, 13C NMR, UV-vis spectroscopy, infrared (IR) and X-ray single crystal crystallography. Density Function Theory (DFT) optimization of molecular structure of 4 was obtained using B3LYP/6-31G(d,p) basis set and the geometrical parameters showed good agreement with the X-ray experimental data. Also, natural bond orbital (NBO) calculations were used to calculate the intramolecular charge transfer (ICT) interactions among the most significant natural orbitals as well as the natural atomic charges. The high LP(N) → BD*(2)C-C/BD*(2)C-N ICT interaction energies indicated strong electron delocalization from the lone pair (LP) of the N-atoms to π*-NBOs of the neighboring Cdbnd C and Cdbnd N bonds. In addition, Time Domain-Density Function Theory (TD-DFT) calculations of the absorption spectrum of 4 predicted that the lowest energy transition is mainly due to HOMO → LUMO excitation (85%), having a wavelength at maximum absorption of 350.5 nm (f = 0.4072) with about 10% relative accuracy compared to the experimental value (391 nm). Furthermore, the most reactive electrophilic and nucleophilic sites in the compound were shown by the molecular electrostatic potential map. The results show strong positive electrostatic potential regions related to the electropositive bivalent sulfur atoms.

  1. Isolation and Purification of Cu-free Methanobactin from Methylosinus trichosporium OB3b

    Energy Technology Data Exchange (ETDEWEB)

    M Pesch; I Christl; K Barmettler; S Kraemer; R Kretzschmar

    2011-12-31

    The isolation of highly pure copper-free methanobactin is a prerequisite for the investigation of the biogeochemical functions of this chalkophore molecule produced by methane oxidizing bacteria. Here, we report a purification method for methanobactin from Methylosinus trichosporium OB3b cultures based on reversed-phase HPLC fractionation used in combination with a previously reported resin extraction. HPLC eluent fractions of the resin extracted product were collected and characterized with UV-vis, FT-IR, and C-1s NEXAFS spectroscopy, as well as with elemental analysis and ESI-MS. The results showed that numerous compounds other than methanobactin were present in the isolate obtained with resin extraction. Molar C/N ratios, mass spectrometry measurements, and UV-vis spectra indicated that methanobactin was only present in one of the HPLC fractions. On a mass basis, methanobactin carbon contributed only 32% to the total organic carbon isolated with resin extraction. Our spectroscopic results implied that besides methanobactin, the organic compounds in the resin extract comprised breakdown products of methanobactin as well as polysaccharide-like substances. Our results demonstrate that a purification step is indispensable in addition to resin extraction in order to obtain pure methanobactin. The proposed HPLC purification procedure is suitable for semi-preparative work and provides copper-free methanobactin.

  2. Climatology of contribution-weighted tropical rain rates based on TRMM 3B42

    Science.gov (United States)

    Venugopal, V.; Wallace, J. M.

    2016-10-01

    The climatology of annual mean tropical rain rate is investigated based on merged Tropical Rainfall Measuring Mission (TRMM) 3B42 data. At 0.25° × 0.25° spatial resolution, and 3-hourly temporal resolution, half the rain is concentrated within only ˜1% of the area of the tropics at any given instant. When plotted as a function of logarithm of rain rate, the cumulative contribution of rate-ranked rain occurrences to the annual mean rainfall in each grid box is S shaped and its derivative, the contribution-weighted rain rate spectrum, is Gaussian shaped. The 50% intercept of the cumulative contribution R50 is almost equivalent to the contribution-weighted mean logarithmic rain rate RL¯ based on all significant rain occurrences. The spatial patterns of R50 and RL¯ are similar to those obtained by mapping the fraction of the annual accumulation explained by rain occurrences with rates above various specified thresholds. The geographical distribution of R50 confirms the existence of patterns noted in prior analyses based on TRMM precipitation radar data and reveals several previously unnoticed features.

  3. A Search for Hα Absorption around KELT-3 b and GJ 436 b

    Science.gov (United States)

    Cauley, P. Wilson; Redfield, Seth; Jensen, Adam G.

    2017-02-01

    Observations of extended atmospheres around hot planets have generated exciting results concerning the dynamics of escaping planetary material. The configuration of the escaping planetary gas can result in asymmetric transit features, producing both pre- and post-transit absorption in specific atomic transitions. Measuring the velocity and strength of the absorption can provide constraints on the mass loss mechanism, and potentially clues to the interactions between the planet and the host star. Here we present a search for Hα absorption in the circumplanetary environments of the hot planets KELT-3 b and GJ 436 b. We find no evidence for absorption around either planet at any point during the two separate transit epochs for which each system was observed. We provide upper limits on the radial extent and density of the excited hydrogen atmospheres around both planets. The null detection for GJ 436 b contrasts with the strong Lyα absorption measured for the same system, suggesting that the large cloud of neutral hydrogen is almost entirely in the ground state. The only confirmed exoplanetary Hα absorption to date has been made around the active star HD 189733 b. KELT-3 and GJ 436 are less active than HD 189733, hinting that exoplanet atmospheres exposed to EUV photons from active stars are better suited for detection of Hα absorption.

  4. Photo-Fenton discoloration of the azo dye X-3B over pillared bentonites containing iron.

    Science.gov (United States)

    Li, Yimin; Lu, Yueqing; Zhu, Xiaoliu

    2006-05-20

    Both Fe pillared bentonite (Fe-B) and Al-Fe pillared bentonite (Al/Fe-B) were prepared and used as heterogeneous catalysts for the photo-Fenton discoloration of azo dye X-3B under UV irradiation. The catalysts were characterized by XRD, BET and TEM. The effects of solution pH, H(2)O(2) concentration, dye concentration and catalyst loading on the rate of discoloration were investigated in detail. The results indicate that the Fe-B and Al/Fe-B have high BET surface area (114.6 and 194.2 m(2)/g, respectively). Both the heterogeneous photo-Fenton processes employing the Fe-B or the Al/Fe-B as catalyst exhibit higher photo-catalytic activity compared to their corresponding homogeneous photo-Fenton process. The amount of Fe ions leached from the Al/Fe-B into the solution is much lower than that leached from the Fe-B during the reaction process.

  5. Photo-Fenton discoloration of the azo dye X-3B over pillared bentonites containing iron

    Energy Technology Data Exchange (ETDEWEB)

    Li Yimin [Department of Chemistry, Shaoxing University, Zhejiang Shaoxing 312000 (China)]. E-mail: liymj@mail.sxptt.zj.cn; Lu Yueqing [Department of Chemistry, Shaoxing University, Zhejiang Shaoxing 312000 (China); Zhu Xiaoliu [Department of Chemistry, Shaoxing University, Zhejiang Shaoxing 312000 (China)

    2006-05-20

    Both Fe pillared bentonite (Fe-B) and Al-Fe pillared bentonite (Al/Fe-B) were prepared and used as heterogeneous catalysts for the photo-Fenton discoloration of azo dye X-3B under UV irradiation. The catalysts were characterized by XRD, BET and TEM. The effects of solution pH, H{sub 2}O{sub 2} concentration, dye concentration and catalyst loading on the rate of discoloration were investigated in detail. The results indicate that the Fe-B and Al/Fe-B have high BET surface area (114.6 and 194.2 m{sup 2}/g, respectively). Both the heterogeneous photo-Fenton processes employing the Fe-B or the Al/Fe-B as catalyst exhibit higher photo-catalytic activity compared to their corresponding homogeneous photo-Fenton process. The amount of Fe ions leached from the Al/Fe-B into the solution is much lower than that leached from the Fe-B during the reaction process.

  6. Journal of Fundamental and Applied Sciences - Vol 4, No 1 (2012)

    African Journals Online (AJOL)

    Journal of Fundamental and Applied Sciences - Vol 4, No 1 (2012) ... Spectroscopic Proprieties of Crystals Mf2 (M=Cd, Sr, Ba) Doped to Rare Earth Ions ... of Borovanadate Glass of Sodium by Raman Spectroscopy Low Frequencies · EMAIL ...

  7. Science, Technology and Arts Research Journal - Vol 2, No 4 (2013)

    African Journals Online (AJOL)

    Science, Technology and Arts Research Journal - Vol 2, No 4 (2013) ... Synteny Approach of Drug Target Prediction among Unique Hypothetical Proteins ... Family's regarding Child Nutrition (under 5 years of age) in Nekemte Town, Ethiopia ...

  8. Siim Nestor soovitab : Tallinn Doom Night vol.1. Odessa Pop / Siim Nestor

    Index Scriptorium Estoniae

    Nestor, Siim, 1974-

    2006-01-01

    Soome doom-metal ansambel Reverend Bizarre üritusel "Tallinn Doom Night vol.1" 8. dets. klubis Rockstar's. Indipoppi viljelev Rootsi duo My Darling Yoy! üritusel "Odessa Pop" 9. dets. Tallinnas klubis KuKu

  9. Arvustuse piirid ja auhinnad : mõtteid kirjanduskriitikast vol 2 / Jan Kaus

    Index Scriptorium Estoniae

    Kaus, Jan, 1971-

    2007-01-01

    Tänapäeva eesti kirjanduskriitikast. Vt. ka vol. 1: Kaus, Jan. Eetika, taburetiefekt ja jätkuv objektivisatsioon, Sirp, 15. juuni., lk. 7. Vastukaja: Raudam, Toomas. Arvustuse piiritused //Sirp (2007) 10. aug., lk. 9

  10. Arvustuse piirid ja auhinnad : mõtteid kirjanduskriitikast vol 2 / Jan Kaus

    Index Scriptorium Estoniae

    Kaus, Jan, 1971-

    2007-01-01

    Tänapäeva eesti kirjanduskriitikast. Vt. ka vol. 1: Kaus, Jan. Eetika, taburetiefekt ja jätkuv objektivisatsioon, Sirp, 15. juuni., lk. 7. Vastukaja: Raudam, Toomas. Arvustuse piiritused //Sirp (2007) 10. aug., lk. 9

  11. Journal of Research in National Development - Vol 10, No 3 (2012)

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    Journal of Research in National Development - Vol 10, No 3 (2012) .... Marketing and 21 Century Technological Challenges: The Need for Curricula ... Trade Blocs and the Future of Global Marketing · EMAIL FULL TEXT EMAIL FULL TEXT

  12. African Journal of Range and Forage Science - Vol 13, No 1 (1996)

    African Journals Online (AJOL)

    African Journal of Range and Forage Science - Vol 13, No 1 (1996) ... on Lolium perenne L. pastures under subtropical conditions: herbage quality ... AU Triumph, Johnstone and Kentucky 31 tall fescue pastures versus maize silage in diets for ...

  13. 02 Inkanyiso Vol4N2 Akintayo and BabalolaFM.fm

    African Journals Online (AJOL)

    ian

    involvement, satisfaction and commitment among the workforce in Nigeria. ..... education institution in Malaysia', Journal of Management and Social Sciences, vol. 5, no. ... 'Diversity and emotion: the new frontiers in organizational behaviour.

  14. Inkanyiso: Journal of Humanities and Social Sciences - Vol 8, No 2 ...

    African Journals Online (AJOL)

    Inkanyiso: Journal of Humanities and Social Sciences - Vol 8, No 2 (2016) ... and health promotion · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT ... Maximising the potential of social media to deliver academic library services to students: ...

  15. Siim Nestor soovitab : Tallinn Doom Night vol.1. Odessa Pop / Siim Nestor

    Index Scriptorium Estoniae

    Nestor, Siim, 1974-

    2006-01-01

    Soome doom-metal ansambel Reverend Bizarre üritusel "Tallinn Doom Night vol.1" 8. dets. klubis Rockstar's. Indipoppi viljelev Rootsi duo My Darling Yoy! üritusel "Odessa Pop" 9. dets. Tallinnas klubis KuKu

  16. East and Central African Journal of Surgery - Vol 12, No 2 (2007)

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    East and Central African Journal of Surgery - Vol 12, No 2 (2007) ... Africa: Implication for training · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT ... Breast fine needle aspiration cytology in a Nigerian tertiary hospital · EMAIL FREE FULL ...

  17. African Journal for the Psychological Study of Social Issues - Vol 5 ...

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    African Journal for the Psychological Study of Social Issues - Vol 5, No 2 (2000) ... Influence of locus of control on conformity to authority in a multicultural ... Psychology and the increasing crime rate in Nigeria · EMAIL FULL TEXT EMAIL FULL ...

  18. Science, Technology and Arts Research Journal - Vol 4, No 2 (2015)

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    Science, Technology and Arts Research Journal - Vol 4, No 2 (2015) ..... The Perception of Prospective Teachers toward Stakeholders' Experience in Practicum: The ... Analysis of Factors Affecting Watermelon Production in Ekiti State, Nigeria ...

  19. South African Journal of Geomatics, Vol. 4, No. 3, August 2015 ...

    African Journals Online (AJOL)

    EEUSER

    This research outlines a methodology of GIS analysis for Informal ... all households have facilities like toilets, running water and proper refuse ..... Decision Support System for Planning Wind Farms in Tuscany (Italy)',Renewable Energy, Vol.

  20. Index to Nuclear Safety: a technical progress review by chronology, permuted title, and author. Vol. 11(1)--Vol. 18(6)

    Energy Technology Data Exchange (ETDEWEB)

    Cottrell, W.B.; Klein, A.

    1978-04-11

    This index to Nuclear Safety covers articles published in Nuclear Safety, Vol. 11, No. 1 (January-February 1970), through Vol. 18, No. 6 (November-December 1977). It is divided into three sections: a chronological list of articles (including abstracts) followed by a permuted-title (KWIC) index and an author index. Nuclear Safety, a bimonthly technical progress review prepared by the Nuclear Safety Information Center (NSIC), covers all safety aspects of nuclear power reactors and associated facilities. Over 450 technical articles published in Nuclear Safety in the last eight years are listed in this index.