Specific expression of the vacuolar iron transporter, TgVit, causes iron accumulation in blue-colored inner bottom segments of various tulip petals.

Science.gov (United States)

Momonoi, Kazumi; Tsuji, Toshiaki; Kazuma, Kohei; Yoshida, Kumi

2012-01-01

Several flowers of Tulipa gesneriana exhibit a blue color in the bottom segments of the inner perianth. We have previously reported the inner-bottom tissue-specific iron accumulation and expression of the vacuolar iron transporter, TgVit1, in tulip cv. Murasakizuisho. To clarify whether the TgVit1-dependent iron accumulation and blue-color development in tulip petals are universal, we analyzed anthocyanin, its co-pigment components, iron contents and the expression of TgVit1 mRNA in 13 cultivars which show a blue color in the bottom segments of the inner perianth accompanying yellow- and white-colored inner-bottom petals. All of the blue bottom segments contained the same anthocyanin component, delphinidin 3-rutinoside. The flavonol composition varied with cultivar and tissue part. The major flavonol in the bottom segments of the inner perianth was rutin. The iron content in the upper part was less than that in the bottom segments of the inner perianth. The iron content in the yellow and white petals was higher in the bottom segment of the inner perianth than in the upper tissues. TgVit1 mRNA expression was apparent in all of the bottom tissues of the inner perianth. The result of a reproduction experiment by mixing the constituents suggests that the blue coloration in tulip petals is generally caused by iron complexation to delphinidin 3-rutinoside and that the iron complex is solubilized and stabilized by flavonol glycosides. TgVit1-dependent iron accumulation in the bottom segments of the inner perianth might be controlled by an unknown system that differentiated the upper parts and bottom segments of the inner perianth.

Overexpression of Arabidopsis VIT1 increases accumulation of iron in cassava roots and stems

Science.gov (United States)

Iron is extremely abundant in the soil, but its uptake in plants is limited due to low solubility in neutral or alkaline soils. Plants can rely on rhizosphere acidification to increase iron solubility. AtVIT1 was previously found to be involved in mediating vacuolar sequestration of iron, which indi...

Headache epidemiology in Vitória, Espírito Santo

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Domingues Renan Barros

2004-01-01

Full Text Available This is the first headache survey in the region of Vitória, ES Brazil. A high prevalence of headache sufferers was found (52.8%. Headache was more common among women (63.9% and less common among people older than 55 years old. The type of professional activity was not related with the headache prevalence. The most frequent causal attribution was stress. Most headache sufferers are not under regular medical treatment (9%, and most of them use analgesic drugs without proper orientation. The most used compounds are combinations with caffeine (33% and simple analgesics (52.3%.

Fishes of the Vitória-Trindade Chain: Biodiversity, Biogeography and Evolution

OpenAIRE

Pinheiro, Hudson Tercio

2016-01-01

Studies on the ecology and biogeography of seamounts and oceanic islands have advanced substantially in the last 60 years. However, few seamounts have been scientifically characterized, with basic aspects of their biodiversity still unknown and many hypotheses not empirically tested. Consequently, the role of seamounts in the evolution for marine species is still unclear. In the south Atlantic, the Vitória-Trindade Chain (VTC) extends ca. 1,200 km offshore the Brazilian coast. For a long time...

Avaliação de populações de Butia capitata de Santa Vitória do Palmar Performance of populations of Butia capitata of Santa Vitória do Palmar

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Elisane Schwartz

2010-09-01

Full Text Available O gênero Butia pertence à família Arecaceae e possui cinco espécies com ocorrência no Rio Grande do Sul. A espécie Butia capitata está recebendo atenção especial, não só pelo seu uso no consumo in natura como também em formas processadas. No entanto, mesmo sendo uma espécie que tem sua utilização registrada desde os tempos pré-históricos, vem sendo explorada apenas de modo extrativista, como a maioria das espécies de frutíferas nativas. Além disso, a espécie está seriamente comprometida em médio prazo pela ausência de regeneração natural e com risco muito alto de extinção num futuro próximo. Nesse sentido, o objetivo deste trabalho foi comparar os frutos, a partir de parâmetros químicos e físicos, e também observar os dados produtivos das plantas de três populações de butiazeiros do município de Santa Vitória do Palmar-RS. Os dados foram obtidos em experimentos conduzidos na safra de 2005/2006 e na safra de 2006/2007, em três propriedades localizadas em Santa Vitória do Palmar. Os resultados permitiram verificar que as propriedades e ou variações genéticas entre as populações de Butia capitata avaliadas propiciaram variabilidade para duração do ciclo, coloração da epiderme dos frutos, volume de suco produzido, relação entre sólidos solúveis totais e acidez titulável, características biométricas de fruto e produtividade anual. Uma das populações, denominada Celina, apresentou maior produtividade e rendimento industrial. As populações Celina e São José apresentaram as melhores características biométricas de fruto. A população Aguiar apresentou a melhor relação entre sólidos solúveis totais e acidez titulável.The genus Butia belongs to the Arecaceae family and shows five species occurring in Rio Grande de Sul. The specie Butia capitata requires special attention, not just for its use as fresh consumption but also as processed product. However, even having its use recorded since

Vitamin C (Vit C) added after irradiation reduces the number and alters the spectrum of CD59- mutants in human/CHO AL cells exposed to high LET carbon ions

International Nuclear Information System (INIS)

Vannais, D.B.; Hirai, Y.; Waldren, C.A.; Ueno, A.

2003-01-01

Full text: Miyazaki, Watanabe, Kumagai and colleagues discovered the existence in mammalian cells of long-lived radicals (LLR) with half-lives of minutes to hours. They further showed that concentrations of LLR were increased in a dose dependent manner by X-rays; that LLR were transforming and mutagenic but not clastogenic or lethal; that they were scavenged by Vit C but not by DMSO, and that they occured mainly (>99.8%) in proteins from which they escape by atomic tunneling. They also showed that Vit C added after radiation (but not DMSO) eliminated HPRT mutants in human cells exposed to X-rays. Following on their work, we found that Vit C (5 mM) added 30 min after radiation significantly reduced, but did not eliminate, induction of CD59- mutants in human-CHO hybrid AL cells exposed to high LET carbon beam radiation (NIRS-HIMAC, 290 MeV/nucleon, LET 100 KeV/μ: m). Lethality of the carbon beam was not affected by Vit C. DMSO decreased mutation and killing, only when present during radiation. Lycopene, reported to reduce spontaneous mutation, did not affect radiation killing or mutagenesis. Our findings with Vit C for high LET generally support the results reported for X-rays. Analysis of the spectrum of mutations in CD59- mutant cells isolated after carbon beam irradiation (2.5 Gy), indicates a substantial reduction by post-radiation Vit C in mutants with small mutations and those displaying genomic instability, seen as increased levels of translocations. Our results substantiate a role for LLR in radiation mutagenesis and implicate them in radiation-induced genomic instability

The Role of Combatives Teaching in Physical Education, by Michal Vit and Zdenko Reguli

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Wojciech Cynarski

2016-06-01

Full Text Available Vit, M., & Reguli, Z. (2015. The Role of Combatives Teaching in Physical Education. Brno: Masaryk University. 128 pp. Retrieved from https://munispace.muni.cz/index.php/munispace/catalog/book/694 There is here a new book, dedicated to the application of martial arts elements in physical education. Sequentially are presented authors, the definition of 'combatives', discussed the contents of the book, pointing out its strengths and weaknesses. A short conclusion is finishing the review article.

O universo poético de Vitória Basaia

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Alexandro Uguccioni Romão

2014-01-01

Full Text Available A artista plástica Vitória Basaia é capaz de parir mundos usando matérias primas refugadas pela sociedade. A Casa Basaia é o seu local de moradia e também o seu ateliê. A casa se transformou ao longo dos anos em uma espécie de museu. Nesse artigo, sondamos esse universo a partir da análise das obras, materiais, técnicas e preocupações ontológicas da artista relacionadas à criação. Com base em conceitos de autores como Deleuze, Guattari, Bertoloto, Bachelard e Brandão, observamos como a inquietação da artista se converte em programa de arte e constitui o seu universo poético, territorializado na Casa Basaia.

Sentidos de Vitória/Derrota para os Pais Segundo Atletas do Alto Rendimento

OpenAIRE

Amblard, Isabela; Cruz, Fatima Leite

2015-01-01

Este estudo compreendeu as representações sociais da vitória/derrota para os pais segundo atletas-adolescentes do esporte de alto rendimento, na cidade do Recife. A adolescência é compreendida a partir da Psicologia Social-histórica, e o embasamento teórico-metodológico da Teoria das Representações Sociais abordou os sujeitos em diferentes contextos socioculturais, lugares de pertencimento, experiências, crenças, saberes e sentimentos compartilhados. Adotou-se a perspectiva pluri metodológica...

Crescimento e extração de micronutrientes em abacaxizeiro 'vitória'

OpenAIRE

Feitosa,Hernandes De Oliveira; Amorim,Aiala Vieira; Lacerda,Claudivan Feitosa De; Silva,Flávio Batista Da

2011-01-01

No presente estudo, foram avaliadas a produção de massa seca e a extração de micronutrientes do abacaxizeiro 'Vitória', adubado com micronutrientes aplicados via solo e por adubação foliar. O experimento foi instalado em abril de 2009, em uma área de 0,178 ha localizada no Perímetro Irrigado Baixo Acaraú, na região norte do Estado do Ceará. O delineamento estatístico empregado foi o de blocos casualizados, no arranjo de parcelas subsubdivididas, com quatro doses de FTE-12 (parcelas), quatro n...

Criminalidade violenta e fragmentação urbana na Grande Vitória

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Cláudio Zanotelli

2006-01-01

Full Text Available Esse texto trata da análise e representa-ção de índices dos homicídios nos bairros das municipalidades da Aglomeração de Vitória no Espírito Santo. Partimos do pressuposto que os índices dos homicí-dios são mais elevados em alguns bairros com certo grau de segregação sócio-espacial. Configura-se assim uma organização espacial dos homicídios fundada na desigualdade sócio-econômica e em certos aspectos estruturais e conjunturais dessas zonas urbanas.

Fish biodiversity of the Vitória-Trindade Seamount Chain, southwestern Atlantic: an updated database.

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Hudson T Pinheiro

Full Text Available Despite a strong increase in research on seamounts and oceanic islands ecology and biogeography, many basic aspects of their biodiversity are still unknown. In the southwestern Atlantic, the Vitória-Trindade Seamount Chain (VTC extends ca. 1,200 km offshore the Brazilian continental shelf, from the Vitória seamount to the oceanic islands of Trindade and Martin Vaz. For a long time, most of the biological information available regarded its islands. Our study presents and analyzes an extensive database on the VTC fish biodiversity, built on data compiled from literature and recent scientific expeditions that assessed both shallow to mesophotic environments. A total of 273 species were recorded, 211 of which occur on seamounts and 173 at the islands. New records for seamounts or islands include 191 reef fish species and 64 depth range extensions. The structure of fish assemblages was similar between islands and seamounts, not differing in species geographic distribution, trophic composition, or spawning strategies. Main differences were related to endemism, higher at the islands, and to the number of endangered species, higher at the seamounts. Since unregulated fishing activities are common in the region, and mining activities are expected to drastically increase in the near future (carbonates on seamount summits and metals on slopes, this unique biodiversity needs urgent attention and management.

Comparision of the Efficacy of Vit B1 (Thiamine and Ibuprofen in Treatment of Primary Dysmenorrhea in Young Girls, Yazd, 2003

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L Sakhavat

2005-04-01

Full Text Available Introduction: This study was performed in order to compare the efficacy of vitamin B1and Iboprofen in the treatment of primary dysmenorrhea. Methods: 180 women aged between 11-17 years with primary dysmenorrhea were divided to 2 groups: Group A: (n: 60 received 100mg vit B1 daily in leuteal phase for 3 months . Grope B :( n: 60 Received 400mg Ibuprofen 3 times/day for 5days (3 days before and 2 days after start of menses for 3 months. Results: 80% of group A and 88.4% of group B were treated as compared to control (P=0001 , but in group B, side effects were more and the regimen was less tolerated as compared to group A. Conclusion: Vit B (Thiamine is well tolerated and effective drug without side effects in primary dysmenorrhea.

Carbon storage and sequestration by trees in VIT University campus

Science.gov (United States)

Saral, A. Mary; SteffySelcia, S.; Devi, Keerthana

2017-11-01

The present study addresses carbon storage and sequestration by trees grown in VIT University campus, Vellore. Approximately twenty trees were selected from Woodstockarea. The above ground biomass and below ground biomass were calculated. The above ground biomass includes non-destructive anddestructive sampling. The Non-destructive method includes the measurement of height of thetree and diameter of the tree. The height of the tree is calculated using Total Station instrument and diameter is calculated using measuring tape. In the destructive method the weight of samples (leaves) and sub-samples (fruits, flowers) of the tree were considered. To calculate the belowground biomass soil samples are taken and analyzed. The results obtained were used to predict the carbon storage. It was found that out of twenty tree samples Millingtonia hortensis which is commonly known as Cork tree possess maximum carbon storage (14.342kg/tree) and carbon sequestration (52.583kg/tree) respectively.

New solutions for effective access powerline solutions. The European smart grid project DLC+VIT4IP; Neue Ansaetz fuer leistungsfaehige Access-Powerline-Loesungen. Das Europaeische Smart-Grid Projekt DLC+VIT4IP

Energy Technology Data Exchange (ETDEWEB)

Offner, Georg [devolo AG, Aachen (Germany)

2011-07-01

By the European DLC+VIT4IP project the development of innovative smart grid solutions for a better handling of energy resources is forced. Smart grid ensures the stable operation of a decentralized electric supply network, where more and more small suppliers contribute by solar or wind energy technology. Business customers as well as private customers benefit from smart grid, as they get instant information about their actual consumption by the Internet. Covered by the project there will be developed new approaches of access powerline communications which provide an effective, IPv6 based communication e.g. between electric meters at home and the power net station. (orig.)

Mortalidade por raça/cor: evidências de desigualdades sociais em Vitória (ES, Brasil Mortality by race/color: evidence of social inequalities in Vitória (ES, Brazil

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Nathalia Modenesi Fiorio

2011-09-01

Full Text Available OBJETIVO: Analisar a mortalidade por causa básica, sexo e raça/cor a partir do sistema de informações sobre mortalidade (SIM, em Vitória (ES, no período de 2003 a 2006. MÉTODOS: Foram calculados e analisados os coeficientes de mortalidade, segundo causa básica e sexo por raça/cor, bem como a idade média e mediana de óbito por causa básica, sexo e raça/cor. Foi calculado o risco relativo (RR por sexo, idade e causa básica (pOBJECTIVE: To analyze mortality by cause and sex among groups of race or color from the mortality information system (MIS in Vitória (Brazil, in the period from 2003 to 2006. METHODS: We calculated and analyzed the mortality rates according to underlying cause, sex and race/color, and the mean and median age of death by underlying cause, sex and race. We calculated the relative risk (RR for age, sex and underlying cause (p<0.05 and CI 95%. RESULTS: The completeness of race/color in SIM ranged from 1% in 1996 to 81% in 2006. There was a greater RR of death among blacks for mental and behavioral disorders (RR=9.29, Ill-defined causes (RR=8.71, and external causes (RR=5.71. For black women, we highlight the external causes (RR=2.38. We found a variation of up to 33 years (nervous system between whites and blacks. CONCLUSION: This study confirms the existence of unequal racial/ethnic mortality, highlighting the mortality from mental disorders and external causes, in addition to early mortality that occurs in the black population.

The AquaVIT-1 intercomparison of atmospheric water vapor measurement techniques

Science.gov (United States)

Fahey, D. W.; Gao, R.-S.; Möhler, O.; Saathoff, H.; Schiller, C.; Ebert, V.; Krämer, M.; Peter, T.; Amarouche, N.; Avallone, L. M.; Bauer, R.; Bozóki, Z.; Christensen, L. E.; Davis, S. M.; Durry, G.; Dyroff, C.; Herman, R. L.; Hunsmann, S.; Khaykin, S. M.; Mackrodt, P.; Meyer, J.; Smith, J. B.; Spelten, N.; Troy, R. F.; Vömel, H.; Wagner, S.; Wienhold, F. G.

2014-09-01

The AquaVIT-1 intercomparison of atmospheric water vapor measurement techniques was conducted at the aerosol and cloud simulation chamber AIDA (Aerosol Interaction and Dynamics in the Atmosphere) at the Karlsruhe Institute of Technology, Germany, in October 2007. The overall objective was to intercompare state-of-the-art and prototype atmospheric hygrometers with each other and with independent humidity standards under controlled conditions. This activity was conducted as a blind intercomparison with coordination by selected referees. The effort was motivated by persistent discrepancies found in atmospheric measurements involving multiple instruments operating on research aircraft and balloon platforms, particularly in the upper troposphere and lower stratosphere, where water vapor reaches its lowest atmospheric values (less than 10 ppm). With the AIDA chamber volume of 84 m3, multiple instruments analyzed air with a common water vapor mixing ratio, by extracting air into instrument flow systems, by locating instruments inside the chamber, or by sampling the chamber volume optically. The intercomparison was successfully conducted over 10 days during which pressure, temperature, and mixing ratio were systematically varied (50 to 500 hPa, 185 to 243 K, and 0.3 to 152 ppm). In the absence of an accepted reference instrument, the absolute accuracy of the instruments was not established. To evaluate the intercomparison, the reference value was taken to be the ensemble mean of a core subset of the measurements. For these core instruments, the agreement between 10 and 150 ppm of water vapor is considered good with variation about the reference value of about ±10% (±1σ). In the region of most interest between 1 and 10 ppm, the core subset agreement is fair with variation about the reference value of ±20% (±1σ). The upper limit of precision was also derived for each instrument from the reported data. The implication for atmospheric measurements is that the

Determinação dos superávits do governo central brasileiro: influência da política monetária na ótica de regressões de limiar

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Bernardo P. Schettini

2014-06-01

Full Text Available Este artigo estuda a política fiscal no Brasil levando em consideração a influência da política monetária. A taxa de inflação do IPCA e os gastos com juros como razão do PIB representam variáveis de estado na determinação da sequência de superávits primários pelo Tesouro. Dessa forma, o modelo empírico de reação da política fiscal foi estimado com o auxílio de regressões de limiar. Em face dos resultados encontrados, não surpreende a tolerância do governo com superávits menores. Vale notar que cortes na taxa SELIC possuem efeitos mistos, pois menores despesas de juros possibilitam reduzir os superávits, porém mais inflação torna a política fiscal persistente. O inverso acontece na situação em que o Banco Central reduz a liquidez da economia.

Vilas operárias e higienismo na cidade de Vitória, as iniciativas governamentais no Espírito Santo - Brasil

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Dra. Arq. Luciana Nemer Diniz

2015-12-01

Full Text Available As vilas operárias foram a materialização habitacional da política higienista promovida pelos governos para a transformação das cidades - novos padrões funcionais e formais. O processo de industrialização foi propulsor dessas alterações em busca de um habitat operário compatível com as necessidades básicas de reprodução da força de trabalho. A participação de médicos e engenheiros sanitaristas norteou as reformas urbanas. Em Vitória o engenheiro Saturnino de Brito projetou o “Novo Arrabalde” em 1896 onde previu duas vilas operárias. A construção de moradia para os empregados remete ao período da escravidão, porém na cidade as vilas operárias estão relacionadas ao período industrial e como o café permaneceu por mais tempo como principal produto da economia capixaba as vilas surgiram a partir da primeira década do século XX. Em Vitória a formação de cortiços foi tímida e não houve corrida no início do século passado por habitação na região central. O aterro e loteamento do Campinho, concluído em 1912 e a construção de vinte e oito casas para funcionários estatais em suas imediações foram às primeiras iniciativas do governo relacionadas à habitação operária na cidade. As vilas operárias em Vitória, suas causas e simbolismos são análises que o presente trabalho propõe-se realizar

Macronutrient uptake, accumulation and export by the irrigated 'vitória' pineapple plant

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Rodinei Facco Pegoraro

2014-06-01

Full Text Available The nutritional state of the pineapple plant has a large effect on plant growth, on fruit production, and fruit quality. The aim of this study was to assess the uptake, accumulation, and export of nutrients by the irrigated 'Vitória' pineapple plant during and at the end of its development. A randomized block statistical design with four replications was used. The treatments were defined by different times of plant collection: at 270, 330, 390, 450, 510, 570, 690, 750, and 810 days after planting (DAP. The collected plants were separated into the following components: leaves, stem, roots, fruit, and slips for determination of fresh and dry matter weight at 65 ºC. After drying, the plant components were ground for characterization of the composition and content of nutrients taken up and exported by the pineapple plant. The results were subjected to analysis of variance, and non-linear regression models were fitted for the significant differences identified by the F test (p N > S > Ca > Mg > P, which corresponded to 898, 452, 134, 129, 126, and 107 kg ha-1, respectively, of total accumulation. The export of macronutrients by the pineapple fruit was in the following decreasing order: K > N > S > Ca > P > Mg, which was equivalent to 18, 17, 11, 8, 8, and 5 %, respectively, of the total accumulated by the pineapple. The 'Vitória' pineapple plant exported 78 kg ha-1 of N, 8 kg ha-1 of P, 164 kg ha-1 of K, 14 kg ha-1 of S, 10 kg ha-1 of Ca, and 6 kg ha-1 of Mg by the fruit. The nutrient content exported by the fruits represent important components of nutrient extraction from the soil, which need to be restored, while the nutrients contained in the leaves, stems and roots can be incorporated in the soil within a program of recycling of crop residues.

Study on the changes of serum levels of PINP, ICTP and 25-OH-VitD3 in pre-menopausal women and their relationship with BMD changes

International Nuclear Information System (INIS)

Zhang Jinshan; Luo Liangping

2010-01-01

Objective: To study the changes of serum levels of procollagen type I N-terminal propeptide (PINP), pyridinoline cross-linked carboxy-terminal telopeptide of type I collagen (ICTP) and 25-OH-VitD 3 in premenopausal women and their relationship with changes of bone mineral density (BMD). Methods: BMD (at L 1 -L 4 and hip region) was examined with dual energy X-ray absorptiometer (DEXA) and serum levels of PINP, ICTP, 25-OH-VitD 3 were determined with RIA in 67 pre-menopausal women. Results: (1) Of the 67 women tested, 19 (28.4%) were found to be with low BMD. The mean BMD values in this group were significantly lower than those in the other 48 women. (2) The serum levels of PINP and ICTP in the 19 women with lower BMD (56.9±18.2 μg/L and 3.78±0.83 μg/L respectively) were much higher than those in the other 48 women (43.8±15.1 μg/L and 3.45±0.98 μg/L respectively, for PINP, P 0.05). The serum 25-OH-VitD 3 levels in the two groups of pre-menopausal women were not significantly different (25.6±7.8 ng/ml vs 27.4±9.2 ng/ml, P>0.05). (3) PINP levels were positively correlated with BMD values (r=0.274∼0.402, P 3 levels were not significantly correlated with BMD levels (r=-0.226∼0.083, P>0.05). PINP levels were weakly positively correlated with 25-OH-VitD 3 levels (r=0.395, P<0.05). Conclusion: There was tendency of increasing rate of bone turnover in pre-menopausal women, which might lead to development of osteoporosis. Early detection with BMD and serum markers of bone resorption (e.q. ICTP) measurement would be highly valuable for implement of prophylactic measures. (authors)

[The trajectory of a public service for alcohol and drug addicts in Vitória: the case of the CPTT].

Science.gov (United States)

Dos Reis, Rossana; Garcia, Maria Lúcia Teixeira

2008-01-01

The aim of this case study is to analyze the Center for Prevention and Treatment of Alcohol and Drug Addiction (CPTT)/Psychosocial Care Center in Drugs and Alcohol (CAPS ad), and to reflect about the implementation process of the local addiction recovery policy for drug users and alcoholics. A document research was performed using as sources the CPTTs mid-year/annual and/or management reports and articles about the service. Data were analyzed using a priori and a posteriori content analysis. The CPTT, a health service of the government of the city of Vitória, was created in 1992 as a psychosocial service. The services provided by the CPTT include individual care, daily group activities and reception and follow-up groups. Today, the situation in the CPTT is characterized by precarious employment relations for most of the professionals working there. The trajectory of the implementation of the CPTT in the city of Vitória expresses two contradictory features: on one hand the advances made in the implementation of the public policy for prevention and treatment of drug and alcohol abuse, and on the other hand the challenge posed by the lack of a human resources policy capable of putting in effect the advances proposed by the policy.

[Disclosing the work processes of the community health agents on the Family Health Strategy in Vitória (ES, Brazil)].

Science.gov (United States)

Galavote, Heletícia Scabelo; do Prado, Thiago Nascimento; Maciel, Ethel Leonor Noia; de Cássia Duarte Lima, Rita

2011-01-01

The community health agent (CHA) has a singular role as a "link" between the community and the health service. The main purpose of this descriptive/qualitative study is to evaluate the production of the CHA work processes in the Family Health Strategy in the county of Vitória (ES, Brazil). The research was developed in the health region of Maruípe, a district in the county of Vitória. The researchers invited 14 CHAs to use a half structured interview based on a guide script containing 24 questions. The process of work in health was considered as "a prevention and promotion of health", and the domiciliary visits were considered as being an everyday basic activity. The CHAs withhold several interpretations concerning to the work process and associated to the concept of unit the team has. It is also possible to notice a kind of idealism of the competences concerning to the CHA work, thus, causing a feeling of impotence and limitation when facing the challenges imposed by it. It is possible to conclude that the CHA needs tools and abilities that overcome their technical knowledge in order to act in a social scope and also to deal with the health needs of each user.

Estudo das condições técnicas de utilização das águas da ETAR da Praia da Vitória Assessment of technical conditions for use of wastewater from the Praia da Vitória treatment plant

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A. Azevedo

2007-07-01

Full Text Available Este estudo teve como objectivo o estudo das condições técnicas de utilização das águas da ETAR da Praia da Vitória para a rega de espaços. A ETAR da Praia da Vitória produz volumes de águas muito apreciáveis os quais são presentemente lançados no oceano através de um emissário submarino. No entanto é provável que esta água, que agora se perde, possa vir a ser utilizada para a rega de espaços verdes numa perspectiva integrada de gestão sustentável do recurso. Para tal, numa primeira fase avaliaram-se os volumes e caudais das águas residuais gerados e a sua sazonalidade e colheram-se amostras para a sua caracterização físico-química. Instalou-se também um ensaio biológico em vasos, com dois solos de carácter ândico classificados ambos como Vitric Hapludands (SSS, 1999, aos quais se aplicaram três tratamentos distintos sendo estes: água de abastecimento público, efluente da ETAR e uma solução nutritiva preparada em laboratório, com o objectivo de comparar o efeito destes tratamentos na produção de uma gramínea de relvado (Agrostis stolonifera. Os resultados analíticos deixaram clara a importância do valor nutritivo das águas residuais, com teores elevados dos macronutrientes azoto, fósforo, cálcio e magnésio, e evidenciado com base no ensaio biológico onde ocorreu um efeito geral positivo das águas residuais no crescimento das plantas em dois solos que, embora do mesmo tipo, apresentavam um nível de fertilidade diferente, mormente em relação aos macronutrientes fósforo, potássio e cálcio solúveis. O efeito residual no solo resultante da aplicação do efluente da ETAR foi verificado apenas em relação ao magnésio.De main goal of this work is to study the technical conditions of utilization of the treated waters of the wastewater treatment plant of Praia da Vitória to lawn and garden irrigation. The WTP of Praia da Vitória treats significant volumes of water which are presently discarded to

A trajetória de um serviço público em álcool e outras drogas no município de Vitória: o caso do CPTT The trajectory of a public service for alcohol and drug addicts in Vitória: the case of the CPTT

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Rossana dos Reis

2008-12-01

Full Text Available O objetivo desse estudo é analisar o Centro de Prevenção e Tratamento de Toxicômanos (CPTT e refletir o processo municipal de implementação da política de atenção aos usuários de álcool e outras drogas. Foi realizada pesquisa documental tendo como fontes os relatórios semestrais/anuais e/ou de gestão do CPTT e artigos publicados sobre o serviço. Para análise dos dados, utilizou-se a análise de conteúdo, recorrendo a categorias a priori e a posteriori. O CPTT, serviço de saúde da Prefeitura Municipal de Vitória, foi criado em 1992 na perspectiva de um serviço psicossocial. O processo de trabalho abrange o atendimento individual; atividades grupais na Atenção Diária e grupos de acolhimento e acompanhamento. O quadro hoje no CPTT se caracteriza por precarização do vínculo de trabalho da maioria dos profissionais com o CPTT. Os dados apontam que a implementação do CPTT no município de Vitória expressa, contraditoriamente, avanços na política municipal de atenção aos usuários de álcool e outras drogas e o desafio da não implementação de uma política de recursos humanos que efetive os avanços propostos pela política.The aim of this case study is to analyze the Center for Prevention and Treatment of Alcohol and Drug Addiction (CPTT/Psychosocial Care Center in Drugs and Alcohol (CAPS ad, and to reflect about the implementation process of the local addiction recovery policy for drug users and alcoholics. A document research was performed using as sources the CPTTs mid-year/annual and/or management reports and articles about the service. Data were analyzed using a priori and a posteriori content analysis. The CPTT, a health service of the government of the city of Vitória, was created in 1992 as a psychosocial service. The services provided by the CPTT include individual care, daily group activities and reception and follow-up groups. Today, the situation in the CPTT is characterized by precarious employment

[Prenatal patient cards and quality of prenatal care in public health services in Greater Metropolitan Vitória, Espírito Santo State, Brazil].

Science.gov (United States)

Santos Neto, Edson Theodoro dos; Oliveira, Adauto Emmerich; Zandonade, Eliana; Gama, Silvana Granado Nogueira da; Leal, Maria do Carmo

2012-09-01

This study aimed to assess the completeness of prenatal care information on the patients' prenatal care cards, according to coverage by various public health services: Family Health Strategy (FHS), Community-Based Health Workers' Program (CBHWP), and traditional Primary Care Units (PCU) in Greater Metropolitan Vitória, Espírito Santo State, Brazil. In a cross-sectional study, 1,006 prenatal cards were randomly selected from postpartum women at maternity hospitals in the metropolitan area. Completeness of the cards was assessed according to the criteria proposed by Romero & Cunha, which measure the quality on a scale from excellent ( 50% incomplete cards). In general, completion of information on the cards was bad (> 20% incomplete), but cards were filled out better in the FHS than in the CBHWP and PCU, especially for tetanus vaccination (p = 0.016) and gestational weight (p = 0.039). In conclusion, the quality of prenatal care in the public health system in Greater Metropolitan Vitória fails to meet the Brazilian national guidelines for maternal and child health.

Comparative study in vitro of contraction percentage by polymerization of the compound resins: FiltekTMZ350 XT, BRILLIANTTMNG, Vit-l-escence®, when subjected to halogen light, applying the Archimedes' principle

International Nuclear Information System (INIS)

Castro Brenes, Maria Jose; Duran Leon, Luis Edgardo

2013-01-01

The contraction by polymerization in vitro is determined in three resins (Filtek TM Z350 XT, BRILLIANT TM NG, Vit-l-escence®), when exposed to halogen light during 60 seconds and applying the Archimedes' principle. This method has provided to professional the criteria to consider for the selection of the material according to the therapeutic requirements. Archimedes' principle is applied like a tool to determine the contraction by polymerization of the resins; however, human errors may be present due to manipulation, omission or losses of the product during processing. The contraction by polymerization of the resins is estimated, where Filtek TM Z350 XT has been the that least volumetric contraction has experienced, followed by BRILLIANT TM NG and lastly Vit-l-escence®, has been the most is contracted of the three. Differences found between the resins studied are established according to contraction by polymerization and compared with the 'literature' and properties that are offered by fabricators. The characteristics of the resins Filtek TM Z350 XT, BRILLIANT TM NG, Vit-l-escence® are evaluated with respect to properties that have had higher or lower contraction by polymerization, to establish patterns of behavior according to their characteristics [es

JUVENTUDE E TRABALHO: uma análise da implementação do Projovem Urbano no município de Vitória da Conquista

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Flávia Cristina Batista Caires

2017-05-01

Full Text Available RESUMO O presente trabalho é uma pesquisa de natureza qualitativa. Tem como foco principal a compreensão de como ocorreu o processo de implementação do Projovem Urbano no município de Vitória da Conquista, por meio das duas dimensões do Programa Formação Básica e Qualificação Profissional. A pesquisa empírica foi realizada com a utilização de análise documental, grupo focal e entrevista em profundidade. Conclui-se, neste estudo, que as políticas públicas de juventude que objetivam a qualificação profissional apresentam mediações entre as propostas educativas e as realidades do trabalho no âmbito da acumulação flexível. Essa pedagogia das competências inculca nos indivíduos que o problema depende de cada um: trata-se de adquirir o pacote de competências que o mercado exige para o novo cidadão produtivo. Palavras-chave: Juventude. Trabalho. Projovem Urbano. YOUTH AND WORK: an analysis of the implementation of the Urban Projovem in Vitória da Conquista city ABSTRACT This study is a qualitative research. Its main focus was understanding how the process of implementing Projovem Urbano in Vitória da Conquista city, through the two dimensions of the program: Basic Education and Vocational Training. The empirical research was conducted with the use of document analysis, focus groups and in-depth interview. It can be concluded in this study that public policy aimed at youth vocational skills have mediations between the educational proposals and the realities of working in the context of flexible accumulation. This pedagogy of skills inculcated in individuals that the problem depends on each one, it is to acquire the package of skills that the market demands for the new productive citizen. Keywords: Youth. Work. Projovem Urbano. JUVENTUD Y EL TRABAJO: un análisis de la aplicación de la Projovem urbana en la ciudad de Vitória da Conquista RESUMEN Este estudio es una investigación cualitativa. Su principal objetivo era

La consolidación de una práctica original: el reparto del superávit del refitor entre los prebendados de la Catedral de Toledo (S. XVI

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Susana Villaluenga

2013-07-01

Full Text Available Según lo previsto en la normativa interna, cada año en el cabildo catedral de Toledo se repartía entre los canónigos y racioneros el superávit o excedente de tesorería del refitor, la oficina que gestionaba el acervo de rentas más importante de la Corporación. Esta misma costumbre existía en otros cabildos hispánicos y en las universidades. En este último caso era frecuente que se repartiera el superávit entre el arca de la fundación y los catedráticos. El reparto del superávit entre los prebendados de los cabildos catedrales resulta un tema de investigación novedoso. Su interés está justificado inicialmente por la curiosidad que puede suscitar este comportamiento económico tan cercano a los modernos sistemas de retribución utilizados en la empresa y que ponen de manifiesto la preocupación de la corporación por preservar los intereses económicos de sus miembros. A este respecto, podemos decir que la producción bibliográfica es escasa. Únicamente existen ciertas referencias en trabajos de contenidos distintos, que tratan de cuestiones relativas a la norma interna, constituciones o estatutos de estas corporaciones. El trabajo que aquí presentamos analiza el origen del superávit del refitor de la Catedral de Toledo, la forma de reparto y su objeto, así como su evolución durante el siglo XVI. De su análisis concluiremos que el incremento de los ingresos de este departamento no fue empleado en aumentar los gastos, como era costumbre, sino en consolidar el excedente de tesorería para su reparto entre los prebendados, al objeto de potenciar la asistencia al coro, siguiendo la doctrina de concilio de Trento. Así, dicho complemento retributivo premiaba la productividad en el servicio cultual del personal ya existente, evitando comprometer a largo plazo recursos en la dotación de nuevas plazas.

Malignancies in HIV/AIDS patients attending an outpatient clinic in Vitória, State of Espírito Santo, Brazil

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Lauro Ferreira da Silva Pinto Neto

2012-12-01

Full Text Available INTRODUCTION: The present study investigated cancer prevalence and associated factors among HIV-infected individuals attending an AIDS outpatient clinic in Vitória, State of Espírito Santo, Brazil. METHODS: A sectional study was conducted among HIV infected adults attending an AIDS outpatient clinic in Vitória, State of Espírito Santo, Brazil. Demographic, epidemiological and clinical data were abstracted from medical records, including cancer diagnoses; nadir and current CD4 cell count, HIV viral load, time on antiretroviral treatment (ART, type of ART and smoking status. RESULTS: A total of 730 (91.3% patients were included in the study. Median age was 44.0 [interquartile range (IQR: 35-50.3] years; median time since HIV diagnosis was 5.5 years (IQR: 2-10; 60% were male; and 59% were white. Thirty (4.1% cases of cancer were identified of which 16 (53% were AIDS defining cancers and 14 (47% were non-AIDS defining malignancies. Patients diagnosed with cancer presented higher chance of being tobacco users [OR 2.2 (95% CI: 1.04-6.24]; having nadir CD4 ≤200 cells/mm³ [OR 3.0 (95% CI: 1.19-7.81] and higher lethality [OR 13,3 (95% CI: 4,57-38,72]. CONCLUSIONS: These results corroborate the importance of screening for and prevention of non-AIDS defining cancers focus in HIV-infected population, as these cancers presented with similar frequency as AIDS defining cancers.

Análise ecológica dos acidentes e da violência letal em Vitória, ES

OpenAIRE

Bastos,Márcia de Jesus Rocha Pereira; Pereira,Jacira dos Anjos; Smarzaro,Dorian Chim; Costa,Everaldo Francisco; Bossanel,Regina Célia Lobo; Oliosa,Durvalina Maria Sesari; Pereira,Joseanny Gomes Poltronieri; Feitosa,Hideko Nagatani; Costa,Marcilene Favarato da; Oliveira,Fabiano José Pereira de; Fávero,Juliana Lopes; Maciel,Ethel Leonor Noia

2009-01-01

OBJETIVO: Analisar o contexto socioeconômico e sua relação com a incidência espacial da mortalidade devido à violência. MÉTODOS: Foi realizado estudo do tipo ecológico no município de Vitória, ES, de 2000 a 2003, sobre a distribuição espacial da mortalidade por acidentes e violência, com base nas informações populacionais e socioeconômicas. Os dados sobre mortalidade foram relacionados a informações como local de residência da vítima, tipo de ocorrência, sexo e raça/cor. A análise das informa...

Abelhas (Hymenoptera: Apoidea visitantes das flores de urucum em Vitória da Conquista, BA Bees (Hymenoptera: Apoidea visitors of the annatto flowers in Vitória da Conquista, Bahia State, Brazil

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Augusto Jorge Cavalcante Costa

2008-04-01

Full Text Available O urucum é um arbusto da família Bixaceae, utilizado na fabricação de corantes naturais para a indústria alimentícia e cosmética. No Brasil, somente nos últimos 15 anos, houve maior interesse pelo cultivo, pois se tornou uma alternativa agrícola promissora. O presente trabalho teve por objetivo identificar as abelhas visitantes das flores do urucuzeiro em Vitória da Conquista, BA. O trabalho foi conduzido no campo experimental da UESB, em uma lavoura do tipo cultivado Peruana Paulista. A coleta das abelhas visitantes foi feita na época principal de floração do urucueiro: março/abril, das 6h às 18h. Foram coletadas 3019 abelhas de 22 espécies, com predominância na visitação das 8h às 14h em relação ao número de indivíduos e número de espécies capturadas. As espécies mais freqüentes foram: Trigona spinipes (Fabricius, Apis mellifera L., Schwarziana quadripunctata (Lepeletier e Tetragonisca angustula (Latreille. Espécies de maior porte, como Xylocopa frontalis (Olivier, Bombus morio (Swederus e Eulaema nigrita Lepeletier, consideradas como eficientes na polinização da cultura do urucum, não foram abundantes neste estudo.Annatto is a shrub from Bixaceae family, which natural pigment (annatto is widely used in food and cosmetic industries. In Brazil, the interest for this crop started in the last fifteen years, once it became a promising agricultural alternative. This study was aimed at identifing visitor bees of annatto flowers at the agriculture region of Vitória da Conquista (BA. The research was carried out in the experimental field of UESB, in an experimental plot planted with the cv. Peruana Paulista. The visitor bees were collected during the main blooming period: March/April, between 6:00h and 18:00h. A total of 3,019 bees from 22 species was collected, with higher visitation during the period from 8:00 to 14:00h, regarding the number of individuals and species. The species most frequent were Trigona spinipes

Unbegleitete minderjährige Flüchtlinge im Migrationsrecht / Bertold Huber

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Huber, Bertold

2016-01-01

Alaealiste, saatjata sisserändajate õigusliku seisundi probleemidest; laste ja noorte majutamise, hoolduse ja abistamise 28.10.2015 jõustunud seadusest. Täistekst (12 lk.) ainult elektroonilises versioonis

Air pollution and respiratory diseases in the Municipality of Vitória, Espírito Santo State, Brazil Doenças respiratórias e poluição atmosférica no Município de Vitória, Espírito Santo, Brasil

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Hermano Albuquerque de Castro

2007-01-01

Full Text Available In Brazil, increasing air pollution in urban areas has led to a rise in respiratory diseases among children and the elderly and has also been the main cause of hospital admissions. This study aims to evaluate the air pollution levels in Vitória, capital of Espírito Santo State, Brazil, in comparison to Brazilian legislation and recent World Health Organization (WHO guidelines, analyzing the spatial distribution of asthma cases treated at local outpatient services. A descriptive epidemiological study was performed with daily records on air pollution and outpatient treatment for respiratory diseases from 2001 to 2003, for children under 6 years of age. A geographic information system (GIS was used to identify asthma distribution in the municipality. Air pollution levels were relatively low when compared to the Brazilian legislation and WHO guidelines. Only mean PM10 and NO2 exceeded the annual quality standard. The three highest asthma outpatient treatment rates were observed in the districts of São José, Jabou, and Morro do Quadro.No Brasil, o crescimento da poluição do ar em áreas urbanas tem sido responsável pelo aumento das doenças respiratórias em crianças e idosos e a principal causa das internações hospitalares. O presente estudo tem como objetivos avaliar os níveis de poluição do ar no Município de Vitória, Espírito Santo, Brasil, em relação à legislação nacional e o recente padrão proposto pela Organização Mundial da Saúde (OMS e, a distribuição espacial dos casos de asma atendidos nos ambulatórios das unidades básicas de saúde no Município de Vitória. Um estudo epidemiológico descritivo foi realizado para o período de 2001 a 2003 para menores de seis anos. O Sistema Geográfico de Informação foi usado para identificar o padrão de distribuição de asma na municipalidade. Os resultados mostraram que os níveis de poluição de ar no município são relativamente baixos, quando comparados à legisla

Trace metal contamination in estuarine fishes from Vitória Bay, ES, Brazil

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Jean-Christophe Joyeux

2004-09-01

Full Text Available Muscular tissue from wild-caught mullet (Mugil spp. and snook (Centropomus spp. was analyzed by atomic absorption spectrometry to determine muscle contamination levels for cadmium, chromium, copper, lead and zinc and evaluate risks to human health associated with seafood consumption. Fishes were captured by subsistence fishermen in Vitória Bay, a Brazilian tropical estuary with numerous outfalls of untreated industrial and residential sewage. Based on the premisses that subsistence fisherman and local consumer show weak (culinary or other preferences within the taxa studied, analyses were conducted and results are reported for genera. Snook cadmium, chromium, copper and zinc concentrations were positively correlated with size or weight. Mullet chromium concentration decreased with size. Cadmium and lead were higher and zinc lower in mullet than in snook. Summer cadmium and lead concentrations were higher than in winter. Chromium presented concentrations consistently over the legal Brazilian limit for seafood. However, the greatest health concern was probably related to lead concentration, especially in respect to consumption by young children.Tecidos musculares de tainhas (Mugil sp. e robalos (Centropomus sp. foram analisados por espectrometria de absorção atômica para determinar as concentrações dos metais cádmio, cromo, cobre, chumbo e zinco no músculo e avaliar os riscos a saúde humana resultante do consumo do pescado. Todos os indivíduos foram capturados por pescadores de subsistência na Baía de Vitória, um estuário brasileiro com numerosos lançamentos de efluentes não tratados de origem doméstica e industrial. Baseado na presunção que pescador de subsistência e consumidor local mostram pouca preferência (culinária ou outra dentro de cada desses taxa, análises foram conduzidas, e resultados reportados, para gêneros em vez de espécies. Em robalos, as concentrações de cádmio, cromo, cobre e zinco aumentaram

De olhos bem abertos: investigando acuidade visual em alunos de uma escola municipal de Vitória De ojos bien abiertos: investigar la agudeza visual de los alumnos de una escuela municipal en la ciudad de Vitória With opened eyes: evaluating the visual acuity of children at an elementary school in Vitória

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Mariana Rabello Laignier

2010-03-01

Full Text Available Esta pesquisa buscou verificar a acuidade visual dos estudantes de uma escola municipal de Vitória Espírito Santo, por meio da aplicação do teste de acuidade visual, baseado na Escala de Snellen; e encaminhar, para exame especializado no ambulatório de oftalmologia do Hospital Universitário Cassiano Antônio Moraes, as crianças que apresentaram déficit visual. Foi um estudo de abordagem quantitativa que discutiu a acuidade visual relacionada ao sexo, idade, série, uso de lentes corretoras e diagnóstico médico. Das 168 crianças avaliadas, maior número de meninas apresentou baixa acuidade visual, assim como os alunos mais jovens; o uso de lentes predominou naqueles que apresentaram redução da acuidade visual; e 71,4% das crianças encaminhadas ao médico apresentaram algum grau de ametropia. Concluiu-se que o trabalho de prevenção é fundamental para se obter uma ótima saúde visual, e que o profissional de saúde é fundamental no desenvolvimento de projetos voltados para a saúde escolar.Este estudio analiza la agudeza visual de los niños de una escuela municipal en la ciudad de Vitória Espírito Santo, mediante la aplicación de la prueba de agudeza visual. Basado en la escala de Snellen; y remitir para un examen especializado en la clínica de oftalmología del Hospital Universitario Cassiano Antônio Moraes a los niños con trastornos visuales. Desarrolla un estudio de enfoque cuantitativo y se analiza la agudeza visual relacionada con el género, la edad, la serie, el uso de lentes correctoras y el diagnóstico médico. De los 168 niños evaluados, mayor cantidad de las niñas exhibirán baja agudeza visual, así como los grupos de edad más jóvenes; el uso de lentes prevalece sobre las personas con reducción de la agudeza visual; y 71,4% de los niños a que se refiere el médico había cierto grado de ametropia. Se concluyó que el trabajo de la prevención es fundamental para obtener una óptima salud visual, y que el

PRÁTICAS AMBIENTALMENTE CORRETAS EM MEIOS DE HOSPEDAGEM: um estudo sobre a percepção ambiental e perfil dos gestores hoteleiros de Santa Vitória do Palmar/RS

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Siuza Monteiro Guedes

2017-09-01

Full Text Available Este estudo foi realizado no município de Santa Vitória do Palmar, RS, teve por objetivo analisar a percepção das práticas ambientalmente corretas de gestão ambiental e de economia de recursos naturais entre os gestores do setor de hospedagem do Município de Santa Vitória do Palmar. Trata-se de um estudo qualiquantitavo de caráter exploratório, representativo e participante, com aplicação de instrumento de coleta de dados, realizado no período de novembro de 2016 a março de 2017 em empreendimentos do setor de hoteleiro e extra-hoteleiro, na cidade sede do Município e em três dos balneários marítimos do mesmo. Como principal resultado pode ser destacado a falta de planos de gestão ambiental dos empreendimentos pesquisados. O estudo se justifica uma vez que não foram encontrados estudos similares.

Condições de trabalho e saúde dos professores da rede particular de ensino de Vitória da Conquista, Bahia, Brasil Labor and health conditions of private school teachers in Vitória da Conquista, Bahia, Brazil

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Núria Serre Delcor

2004-02-01

Full Text Available A literatura científica sobre a saúde dos professores é escassa e recente, enfocando especialmente o desgaste e estresse. Este trabalho objetivou descrever as condições de trabalho e saúde dos professores da rede particular de ensino da cidade de Vitória da Conquista, Bahia, Brasil. Num questionário auto-aplicado foram coletadas informações de 250 professores de dez escolas. Entre as características do trabalho docente, avaliadas pelo Job Content Questionnaire, destacaram-se ritmo acelerado de trabalho, ser criativo e ter boas relações com as pessoas no trabalho. As queixas de saúde mais freqüentes estavam relacionadas à postura corporal, à saúde mental e a queixas relacionadas à voz. A prevalência de distúrbios psíquicos menores (DPM, medida pelo Self Reporting Questionnaire-20, foi de 41,5% e estava fortemente associada a longos períodos de intensa concentração em uma mesma tarefa e volume excessivo de trabalho. Os resultados sugerem relação entre a prevalência de DPM e algumas características do trabalho docente, evidenciando desgaste psicológico do educador.The scientific literature on teachers' health is scarce, recent, and focuses predominantly on stress and burnout. This study describes the labor conditions of private school teachers in Vitória da Conquista, Bahia State, Brazil. Information on 250 teachers from the ten largest schools in the municipality was collected through a self-applied questionnaire. The most relevant characteristics of teachers' work, evaluated by the Job Content Questionnaire were: speed of work, creativity at work, and relations with colleagues. The most frequent complaints related to posture, mental strain, and voice problems. Prevalence of minor psychological disorders according to the Self Reporting Questionnaire-20 was 41.5%, strongly associated with long periods of intense concentration on the same job and excessive work. Results suggest an association between the prevalence of

Prevalence of Epstein-Barr virus antibodies in healthy children and adolescents in Vitória, State of Espírito Santo, Brazil

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Figueira-Silva Cecília M.

2004-01-01

Full Text Available The prevalence and age distribution of Epstein-Barr virus infection varies in different populations and there is little information about the epidemiology of this infection in Brazil. We studied the prevalence of EBV antibodies in a sample of 283 children and adolescents between 1 and 21 years old. The sample was taken from two neighborhoods in Vitória (capital city of Espirito Santo, Brazil. The São Pedro (SP neighborhood represented an area with lower socioeconomic status and the Praias (P neighborhood represented an area with higher SES. Anti-VCA (Virus Capsid Antigen antibodies were detected by ELISA and anti-EBNA (Epstein-Barr Nuclear Antigen antibodies were detected by an anti-complement immunofluorescence method, both using commercial kits. The results showed an overall prevalence rates of anti-VCA and anti-EBNA of 71% and 54% respectively. The prevalence for both anti-EBV antibodies was higher and probably the infection occurred earlier in the SP neighborhood. Among the various socioeconomic factors studied only low family income and maternal education level were significantly correlated with a higher frequency of positive serology for anti-VCA. These results demonstrate that there is a high prevalence of EBV antibodies in children and adolescents living in Vitória, that occurs more frequently at a younger age in children from families with low socioeconomic status. In addition, the results demonstrate an intermediate age distribution pattern between those reported in developed and underdeveloped countries.

EU obligation to the TRIPS Agreement: EU Microsoft Decision / Sujitha Subramanian

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Subramanian, Sujitha

2010-01-01

Mil määral on kohustatud Euroopa Liit järgima TRIPSi lepingu kohustusi? Microsoft esitas kaebuse, et täielikult toimiva Windowsi versiooni kasutuselevõtul rikuti TRIPSi lepingust tulenevaid reegleid

Intervju s Arvo Iho / Arvo Iho ; interv. Grigori Utgof

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Iho, Arvo

2006-01-01

Andrei Tarkovski õpilane ja praktikandina "Stalkeri" võtetel osalenud režissöör meenutab A. Tarkovski vaateid filmikunstile, Sergei Eisensteini montaazhiteooriale, problemaatikat filmivõtetel, "Stalkeri" esimese versiooni erinevust lõplikust

Üks aadressimärkmik kõige jaoks / Adam Tanner

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Tanner, Adam

2013-01-01

Bart Lorang soovib luua täiuslikku iseuuenevat aadressimärkmikku. Esialgses versioonis on juba 300 miljonit nime Internetis kättesaadavatest avalikest andmetest olemas, kuid andmekaitseeksperdid kahtlevad selle äri seaduslikkuses

Superávit e déficit fiscal dos municípios brasileiros: uma aplicação do modelo de viés de seleção em painel

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Sergio Naruhiko Sakurai

2014-12-01

Full Text Available O objetivo deste artigo é analisar o resultado primário fiscal dos municípios brasileiros entre 1999 e 2007, aplicando a metodologia de viés de seleção para dados em painel proposto por Wooldridge (1995. Mais especificamente, assume-se que a ocorrência de superávits e déficits fiscais não deve ser tomada como um evento puramente aleatório e, alternativamente, assume-se a existência de um primeiro estágio que determina a ocorrência de um superávit ou de um déficit fiscal. Os resultados corrigidos para a presença do viés amostral indicam que saldos fiscais positivos e negativos são parcialmente influenciados pelas características fiscais dos municípios, bem como pela estrutura demográfica da população. Contudo, suas características políticas pouco influenciam o resultado fiscal.

EuroPlus+ Reward / Alar Hammer

Index Scriptorium Estoniae

Hammer, Alar

2000-01-01

EuroPlus+ Reward on mitmes versioonis ilmunud inglise keele õppeprogramm, milles on ühendatud multimeedia võimalused ning distantsõpe ning mis loob täiesti uued võimalused keele omandamiseks arvuti abil

Yield components, yield and seed quality of cowpea in Vitória da Conquista, Bahia = Componentes de produção, produtividade e qualidade de sementes de feijão-caupi em Vitória da Conquista, Bahia.

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Alexandre Carneiro da Silva

2014-12-01

Full Text Available The objective with this study was to evaluate the components of production, productivity and quality of seeds of cowpea cultivars grown in Vitória da Conquista, Bahia. For this, an experiment was conducted in the statistical design of randomized blocks, with eight cultivars (BRS Marataoã, BRS Paraguaçu, BRS Pajeú, BRS Xiquexique, BRS Guariba, BRS Potengi, BRS Cauamé and BRS Itaim and four replications. Were evaluated the yield components: number of pods per plant (NVP; number of seeds per pod (NSV; weight of hundred seeds (P100; percentage of plant survival (%SBV and seed yield (PROD. To evaluate the physiological quality of the seeds, was determined the water content (%U and mass of thousand seeds (MMIL, and performed tests of germination percentage (%G, germination first count (PC, electrical conductivity (CE, percentage (%E and speed index of seedling emergence (IVE. Data were subjected to analysis of variance and mean ratings compared by Tukey test at 5% probability of error, and yield components correlated by the method of Pearson. The high seed yield achieved for all cultivars, averaging 1749.08 kg ha-1, and the good seed quality, which meets the standards of production and marketing indicate that Vitoria da Conquista, Bahia has favorable conditions for production of seeds of cowpea, with sowing in November. The number of pods per plant is the yield component that most influences in seed yield.= - Objetivou-se com este trabalho avaliar os componentes de produção, a produtividade e a qualidade de sementes de cultivares de feijão-caupi cultivadas em Vitória da Conquista, Bahia. Para isso, um experimento foi conduzido no delineamento estatístico de blocos ao acaso, com oito cultivares (BRS Marataoã, BRS Paraguaçu, BRS Pajeú, BRS Xiquexique, BRS Guariba, BRS Potengi, BRS Cauamé e BRS Itaim e quatro repetições. Foram avaliados os componentes de produção: número de vagens por planta (NVP; número de sementes por vagem (NSV

Kai Kaljo video-workshopil Ljubljanas

Index Scriptorium Estoniae

2000-01-01

Video-workshop "Crossing Over 4" 9.-23. XII 1999 Ljubljana meedialaboris. Korraldajad videokunsti professor Nina Czegledy Torontost ja bulgaaria kuraator Iliyana Nedkova. Eestit esindanud Kai Kaljo esitas videos "Pathetique" oma versiooni Beethoveni "Pateetilisest sonaadist"

Arctic Sea jõudis lõpuks Soome tagasi, kuid saladused jäävad / Jaanus Piirsalu

Index Scriptorium Estoniae

Piirsalu, Jaanus, 1973-

2010-01-01

Kaubalaeva Arctic Sea kaaperdamises süüdistatavad on endiselt Moskvas eeluurimisvanglas. Kaubalaeva kaaperdajate käest vabastama saadetud Vene sõjalaeva Ladnõi ohvitser esitatas uue versiooni Arctic Sea hõivamise kohta. Kaart

V & A ümberehitus viibib / Maria-Kristiina Soomre

Index Scriptorium Estoniae

Soomre, Maria-Kristiina, 1978-

2001-01-01

Londoni Victoria ja Alberti muuseum on muuseumi ümberehitusprojekti edasi lükanud ja tellinud Daniel Libeskindilt odavama versiooni. Keskendutakse 22. XI avatavatele galeriidele ja uuele väljapanekule. Alates 22. XI on külastamine tasuta.

Mängufilm 2001.aasta 11. septembri katastroofist on USA-s edukas

Index Scriptorium Estoniae

2006-01-01

Paul Greengrassi mängufilm "United 93" annab oma versiooni sündmustest lennukis, mis pidi terroristide soovil sööstma kas Valgesse Majja või Kapitooliumi. Avanädalavahetusel kogus film peaaegu 12 miljonit dollarit

Kuulus Roman Viktjuki Teater Tallinnas / Meelis Kapstas

Index Scriptorium Estoniae

Kapstas, Meelis, 1963-

2000-01-01

26. ja 27. mail Vene Kultuurikeskuses Roman Viktjuki Teatri külalisetendused. Mängitakse R. Viktjuki lavastust "Kellavärgiga apelsin" Anthony Burgessi romaani järgi ja Viktjuki versiooni Oscar Wilde'i kurbmängust "Salomé"

Takoi zavodnoi i izõskannõi

Index Scriptorium Estoniae

2000-01-01

26. ja 27. mail Vene Kultuurikeskuses Roman Viktjuki Teatri külalisetendused. Mängitakse R. Viktjuki lavastust "Kellavärgiga apelsin" Anthony Burgessi romaani järgi ja Viktjuki versiooni Oscar Wilde'i kurbmängust "Salomé"

An interpretation of body care by users of a public health system program in Vitória/ES

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Thacia Varnier

2016-05-01

Full Text Available This paper addresses topics on Health Education and risk behavior. Research was carried out to investigate how users deal with information received from a Health Education program, in Vitória (Espírito Santo, Brazil, focused on intervention in overweight and obese individuals. The methodological tools used were participant observation, semi-structured interviews and experimentation with the technique of Body-Map Storytelling. We conclude that the individuals showed evidence of conformity by absorbing and conveying the behavior recommended by experts to their daily lives. We believe that this finding is linked to the fact that, in contemporary society, the model of living well corresponds to healthy lifestyle, in which the “naturalization” of these ideals ultimately contributes to the acceptance of these norms due to the belief that this would be the only right way of living.

Floristica da restinga de Camburi, Vitória, ES The flora of Camburi restinga, Vitória, Espírito Santo State, Brazil

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Oberdan José Pereira

2000-04-01

Full Text Available Este trabalho consistiu no levantamento florístico e classificação da vegetação de restinga em Camburi, Vitória, ES. Foram realizadas coletas mensais na área de estudo, que totalizaram 211 espécies distribuídas em 70 famílias, sendo Fabaceae (19 espécies, Myrtaceae (14, Euphorbiaceae (10, Rubiaceae (10, Cyperaceae (9, Sapindaceae (7 e Lauraceae (7 as mais importantes quanto ao número de espécies. A região apresenta remanescentes das comunidades mata seca, aberta de Ericaceae e brejo herbáceo, além de áreas degradadas com espécies pioneiras. A maioria das espécies possui ampla distribuição pela costa brasileira, no entanto, outros padrões foram encontrados. Erythroxylum tênue Plowman, Ocotea nutans (Nees Mez, Miconia brevipes Benth., Prescottia plantaginea Lindl., Pseudolaelia vellozicola (Hoehne Porto & Brade e Coccocypselum hirsutum Bartl. ex DC. são citadas pela primeira vez para as restingas do Espírito Santo.The flora of the Camburi restinga in the municipality of Vitoria, Espírito Santo State, Brazil, was surveyed and vegetation types were classified. Monthly visits to the area resulted in a list of 211 species from 70 plant families of which the most important, according to species richness, were Fabaceae (19, Myrtaceae (14, Euphorbiaceae (10, Rubiaceae (10, Cyperaceae (9, Sapindaceae (7 and Lauraceae (7. This coastal plain still supports remnant patches of dry restinga forest, open Ericaceae scrub and sedge marsh, as well as disturbed areas dominated by pioneer species. Most of the species are widely distributed along the Brazilian coast other patterns, however, being found. Erythmxylum tenue Plowman, Ocotea nutans (Nees Mez, Miconia brevipes Benth., Prescottia pilntaginea Lindl., Pseudolaelia vellozicola (Hoehne Porto & Brade and Coccocypselum hirsutum Bartl. ex DC. are reported for the first time in the restingas of Espírito Santo.

Eesti vahekeele korpus / Pille Eslon

Index Scriptorium Estoniae

Eslon, Pille, 1950-

2014-01-01

Tutvustatakse eesti vahekeele korpuse esimest versiooni, mis on valminud Tallinna Ülikoolis, õppijakeele automaatse analüüsi võimalusi, korpuspõhiste meetodite rakendamist ning antakse lühiülevaade korpuse põhjal tehtud uurimistööst

Criadouros de Aedes (Stegomyia aegypti (Linnaeus, 1762 em bromélias nativas na Cidade de Vitória, ES Aedes (Stegomyia aegypti (Linnaeus, 1762 breeding sites in native bromeliads in Vitória City, ES

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José Benedito Malta Varejão

2005-05-01

Full Text Available Alguns insetos transmissores de doenças procriam exclusivamente nas proximidades das residências. O Aedes aegypti, responsável por epidemias de dengue em cidades brasileiras, representa sério risco também para a febre amarela. Com o insucesso da campanha de erradicação do inseto, justifica-se a busca de criadouros fora do alcance das medidas de controle atualmente adotadas. Na Cidade de Vitória, ES, investigou-se a ocorrência de criadouros de Aedes aegypti na água coletada em bromélias nativas, sobre as rochas. Paralelamente, avaliou-se a infestação predial nas áreas urbanas contíguas. Em quatro das cinco áreas investigadas foram encontradas larvas de culicídeos nas bromélias, sendo que em duas foi identificado Aedes aegypti. A presença dos criadouros em bromélias não guardou relação com a infestação predial nas áreas próximas. Torna-se necessário definir se os criadouros em bromélias constituem focos primários do Aedes aegypti, ou se representam uma conseqüência da elevada infestação urbana.Some insects that are vectors of human diseases have accompanied man in his migrations throughout the world and breed exclusively in the proximity of human dwellings. The mosquito Aedes aegypti has been responsible for epidemics of dengue in Brazil and its presence also constitutes a serious risk for future outbreaks of urban yellow fever. The failure of campaigns to eradicate this species justifies the search for alternative breeding sites, which may be beyond the reach of present control measures. In this study the occurrence of Aedes aegypti breeding sites in native bromeliads on rocky slopes was investigated in five areas of Vitória, capital of the Brazilian State of Espírito Santo, ES. Water contained in the bromeliads was collected with the aid of a suction apparatus to search for culicid larvae. The degree of infestation of buildings in adjacent urban areas was evaluated simultaneously. Culicid larvae were found in

Potential application of immobilized streptokinase extracted from Streptococcus equinus VIT_VB2.

Science.gov (United States)

Vaishnavi, B; Subathra Devi, C

2017-11-26

Streptokinase purified from Streptococcus equinus VIT_VB2 isolated from bovine milk sample was immobilized in various solid supports namely entrapment in agarose gel, calcium alginate beads and gelatin gel by cross-linking with formaldehyde. Immobilization of streptokinase in calcium alginate beads showed maximum efficiency (81.8 ± 1.06%) when compared with entrapment with agarose gel (55.6 ± 2.17%) and cross-linked gelatin formaldehyde gel (71.0 ± 1.54%). The purified SK activity was expressed maximum in calcium alginate (1%) and gelatin gel (0.25%) with 1292.68 ± 1.33 and 1121.9 ± 1.2 U mL -1 , respectively. Similarly, SK entrapped in gelatin gel and calcium alginate showed maximum in vitro blood clot lysis activity with 77.67 ± 2.64% and 76.16 ± 2.72%, respectively. The immobilized SK in gelatin gel showed complete clot lysis within 15 min; hence, this application of the study could be used in the treatment of superficial thrombophlebitis, phlebitis, and venous thrombosis. These beads were used for three repeated cycles to check the conversion of substrates into their products, and we concluded that SK can be immobilized in the suitable matrices. Therefore, this helps in the drug-delivery strategies in highly efficient way, moreover, economically competent process in the pharmaceutics.

Ilmus esimene mahukam eestikeelne teatriajaloo ülevaade / Andres Laasik

Index Scriptorium Estoniae

Laasik, Andres, 1960-2016

2007-01-01

25. jaan. esitleti Eesti Teatriliidus "Oxfordi illustreeritud teatriajaloo" eestikeelset versiooni. Tõlkeraamatu aluseks on 1995. aastal kirjastuses Oxford University Press ilmunud samanimeline raamat, mille koostajaks on John Russell Brown. Teose on tõlkinud Anne Lange, Ilona Kolberg, Piret Kruuspere, Kristjan Jaak Kangur

Desempenho do abacaxizeiro 'Vitória' em resposta à aplicação de ácidos húmicos durante a aclimatação Performance of 'Vitória' pineapple in response to humic acid application during acclimatization

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Lílian Estrela Borges Baldotto

2009-08-01

Full Text Available A aclimatação de mudas de abacaxizeiro propagadas por cultura in vitro é um processo lento. A promoção do crescimento radicular pelo uso de ácidos húmicos pode ser útil durante esse processo. O objetivo deste trabalho foi avaliar o desempenho do abacaxizeiro 'Vitória' propagado por cultura de tecidos em resposta à aplicação de diferentes doses de ácidos húmicos isolados de vermicomposto e de torta de filtro durante o período de aclimatação em casa de vegetação. Foram avaliadas as características anatômicas da lâmina foliar, o teor de pigmentos fotossintéticos, o crescimento da parte aérea e do sistema radicular, e o conteúdo de nutrientes foliares. Os resultados mostraram incrementos no crescimento e desenvolvimento da parte aérea e do sistema radicular com a aplicação de ácidos húmicos, bem como acúmulo significativo de N, P, K, Ca e Mg e aumento na relação clorofila a/clorofila b. A promoção do crescimento das mudas durante o período de aclimatação, em resposta à aplicação de ácidos húmicos, pode melhorar a adaptação do abacaxizeiro ao ambiente ex vitro.The acclimatization of pineapple plantlets propagated by plant tissue culture is slow. The intensification of root growth by the use of humic acids may be useful during this process. The aim of this study was to evaluate the greenhouse acclimatization of 'Vitória' pineapple propagated by plant tissue culture in response to different doses of humic acids isolated from vermicompost and filter cake. Leaf anatomy, photosynthetic pigment content, shoot and root growth, and leaf nutrients were evaluated. An increase in growth and development of shoots and root system with the application of humic acids as well as a significant accumulation of N, P, K, Ca, and Mg and increase in the chlorophyll a / chlorophyll b ratio were found. Growth promotion by the application of humic acids to plantlets during the period of acclimatization can improve their

Efeito da safra vitícola na composição da uva, do mosto e do vinho Isabel da Serra Gaúcha, Brasil

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Rizzon Luiz Antenor

2006-01-01

Full Text Available Entre os fatores que interferem na composição e na qualidade do vinho, destaca-se a safra vitícola, com suas variações climáticas. Com este objetivo, avaliou-se o efeito das safras de 1999, 2000 e 2001 na composição da uva, do mosto e do vinho Isabel da Serra Gaúcha. Nesse sentido, foram selecionados vinte e dois produtores de uva Isabel de onze municípios dessa região. Por ocasião da maturação, para as avaliações da uva e do mosto e a elaboração das microvinificações, foram colhidos 60kg de uva de cada viticultor. A safra vitícola interferiu significativamente no peso do cacho e da baga, mas não alterou a relação peso da ráquis/peso do cacho. No mosto, observaram-se diferenças em todas as variáveis, exceto para a relação degreesBrix/acidez total. No vinho, teve efeito na maior parte das variáveis avaliadas, com exceção aos cátions K, Mn, Cu e Rb. Em geral, a safra de 1999 foi a melhor, pois apresentou parâmetros mais adequados de acidez total, extrato seco, extrato seco reduzido, alcalinidade das cinzas, taninos, antocianinas e glicerol, variáveis essas que determinam a estrutura e a cor dos vinhos, importantes fatores para sua qualidade.

Fungos micorrízicos arbusculares em diferentes coberturas florestais em Vitória da Conquista, Bahia

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Renata Soares dos Santos

Full Text Available Os fungos micorrízicos arbusculares (FMAs são organismos mutualistas obrigatórios, que facilitam a sobrevivência e o desenvolvimento de plantas. São influenciados pela cobertura florestal e por outros fatores bióticos e abióticos. O objetivo deste trabalho foi avaliar a composição e a diversidade dos FMAs em solo sob três diferentes coberturas florestais, em Vitória da Conquista-BA. O plantio de Madeira-nova apresentou maior abundância de esporos que o plantio de Eucalipto. O fragmento de Mata nativa apresentou um grande número de espécies quando comparado com os plantios, sendo seis exclusivas deste local e com potencial de apresentar uma maior diversidade. Os FMAs apresentaram diferenças marcantes de composição e diversidade nas coberturas florestais avaliadas. A grande abundância de esporos e a homogeneidade amostral das espécies no plantio de Madeira-nova são fatores facilitadores para um futuro isolamento de FMAs.

Tsenseerimata Dorian Gray? / Udo Uibo

Index Scriptorium Estoniae

Uibo, Udo, 1956-

2011-01-01

Harvardi ülikooli kirjastus üllitas 2011. a. kevadel Oscar Wilde'i ainsaks jäänud romaani "Dorian Gray portree" esialgse versiooni, mis on varustatud toimetaja Nicholas Frankeli põhjalike kommentaaridega ja kus eessõna manifesteerib jõuliselt autori esteetilisi vaateid

Zavershilis sjomki "Annõ Kareninoi"

Index Scriptorium Estoniae

2007-01-01

Režissöör Sergei Solovjov lõpetas töö Lev Tolstoi romaani "Anna Karenina" ekraniseeringuga. Lõpptulemusena valmis režissööril kaks versiooni - üks täispikk mängufilm ja teine 5-seerialine teleseriaal

Oodatud & armastatud / Ülli Plink

Index Scriptorium Estoniae

Plink, Ülli, 1973-

2001-01-01

Võrreldakse kaht Alain Boublil'i ja Claude-Michel Schönbergi muusikali "Hüljatud" versiooni : Cameron Macintoshi ja Royal Shakespeare Company koostöös lavale toodud muusikali Londonis ja lavastaja Georg Malviuse tööd. Esietendus 30. oktoobril Tallinna Linnahallis

Avaliação da qualidade de méis de abelha produzidos e comercializados em Vitória da Conquista, Bahia

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Manoel Nelson de Castro Filho

2017-10-01

Full Text Available O mel é um produto elaborado pelas abelhas, a partir do néctar das flores ou de secreções procedentes de partes vivas das plantas, que as abelhas recolhem, transformam e combinam com substâncias específicas próprias. Este alimento é nutritivo e energético, sendo apreciado, entre outros fatores, por suas propriedades terapêuticas. Açúcares, água, vitaminas, ácidos orgânicos e sais minerais são os principais constituintes do mel. Nesse sentido, as análises laboratoriais são utilizadas para inspeção, com o objetivo de conhecer a composição química do mel, identificando a qualidade do produto. O objetivo deste estudo foi avaliar a qualidade dos méis produzidos e comercializados na cidade de Vitória da Conquista - BA e sua adequabilidade em relação à identidade e qualidade padronizadas pela legislação brasileira. As amostras foram obtidas da Casa do Mel da Universidade Estadual do Sudoeste da Bahia, em Vitória da Conquista, e analisadas no laboratório de Química Analítica e Ambiental da mesma instituição. Foram analisados teor de umidade, acidez livre, açúcares redutores e não redutores, hidroximetilfurfural, sólidos solúveis totais, pH e reação de Fiehe e Lund  em 18 amostras de méis. Das amostras avaliadas, 66,67% apresentaram valores de HMF superiores aos aceitáveis pela legislação, 11,11% para acidez e 27,78% para o teste Fiehe. O resultado das demais análises apresentaram-se dentro dos valores permitidos.

Emprego de modelo de susceptibilidade a escorregamentos rasos para gestão de riscos de desastres no município de Vitória-ES / Employment of shallow landslides model for disaster hazard management in the city of Vitória-ES

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Renato Fontes Guimarães

2013-04-01

Full Text Available O Brasil está entre os 10 países mais atingidos por desastres naturais no mundo e os deslizamentos constituem-se num dos principais riscos geológicos do País, muitas vezes condicionado pela ocupação das encostas que provocam alterações ampliando a possibilidade dessas ocorrências. Nesse contexto, apresenta-se como essencial a busca por ferramentas e instrumentos que permitam conhecer os riscos potenciais de determinadas áreas e com isso adotar medidas antecipatórias ou preventivas. Uma dessas ferramentas são os modelos de predição de áreas susceptíveis à escorregamentos. Desta forma, o objetivo deste trabalho é aplicar um modelo matemático de predição de escorregamentos para a gestão de riscos como subsídios às políticas públicas locais no Município de Vitória/ES. Para isso, foi definida como área de estudo a parte do município denominada Ilha de Vitória. As cartas topográficas na escala 1:5.000 foram editadas e utilizadas para a geração do modelo digital de terreno (MDT e dos atributos morfométricos (área de contribuição e declividade. Como parâmetros de solo foram testados oito conjuntos de dados provenientes de áreas semelhantes. Em seguida foi aplicado o modelo Shalstab, que combina o modelo hidrológico com o modelo de estabilidade da encosta e possibilita a definição de áreas susceptíveis a escorregamentos. Os oito modelos foram integrados em uma única imagem a partir do maior valor de instabilidade entre todas as simulações. A partir desse mapa de susceptibilidade de escorregamento foi possível definir as áreas de atenção, prioritárias para o desenvolvimento e a definição de ações de gestão do risco de desastres na região.

Diagnosis of the inadequate disposal of construction and demolition waste in Vitória de Santo Antão, Pernambuco | Diagnóstico da eliminação inadequada de resíduos de construção e demolição em Vitória de Santo Antão, Pernambuco

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Emmanuelle M.G. Lorena

2017-06-01

Full Text Available Construction and Demolition Waste (C&DW, also known as rubble, are increasingly frequent and visible in today's society, due to the large volume generated in construction and its irregular deposition. As potential polluters of the environment, C&DW directly affect the environmental conditions of water bodies and the sanitation of urban areas. In this context, the objective of this work was to diagnose by mapping the presence of irregular deposition of construction residues on urban roads in the city of Vitória de Santo Antão-PE. It was visited in the district of Matriz, totaling 44 streets in the month of June 2016, mapping the locations of irregular deposition by buildings and the size of the work. As a result, it was possible to diagnose the presence of 64 points of clusters of residues in front of the buildings, which are considered to be irregular, since there were no technical personnel responsible for the construction or license plate issued by the municipality in 95% of the points. The works were considered as small, mostly renovations. Likewise, the quantification and the georeferencing of C&DW become essential to enable effective measures in the final deposition and reduction of waste generation. The fulfillment of legal norms is also a factor that contributes to the conservation of the environment, guarantees the urban water flow and does not compromise the landscape of the municipality. As a result of this survey, there is an urgent need to implement a management plan for C&DW in the city of Vitória de Santo Antão.

Teorija Darvina / Maria Tsheskis

Index Scriptorium Estoniae

Tsheskis, Maria

2005-01-01

Peter Jacksoni uusversioon Merian C. Cooperi ja Ernest B. Schoedsacki 1933.a. fantastilisest põnevusfilmist "King Kong" : Ameerika Ühendriigid 2005. Tehnilisi lahendusi hiigelahvi kujutamisel on võrreldud nii esimese filmi kui 1976.a. versiooni (režii John Guillermin) omaga. Loetletud ka teisi samateemalisi linateoseid

Qualidade de vida dos profissionais do setor madeireiro de Vitória da Conquista-BA

Directory of Open Access Journals (Sweden)

Giovanni Correia Vieira

Full Text Available A pesquisa foi desenvolvida no município de Vitória da Conquista, Estado da Bahia, com o objetivo de avaliar a qualidade de vida de funcionários no setor madeireiro, utilizando-se a metodologia WHOQOL-bref. Foram aplicados 100 questionários com 26 questões cada, subdividas em quatro domínios: sete pertencentes ao físico; seis ao psicológico; oito ao meio ambiente, e três referentes às relações sociais, além de duas questões de autoavaliação relacionadas a condições de saúde e qualidade de vida. Nesse último domínio, os funcionaram relataram estar satisfeitos em relação à qualidade de vida. O domínio 'meio ambiente' apresentou menor grau de satisfação, em virtude da elevada concentração de particulados em suspensão, das temperaturas expressivas no ambiente de trabalho e dos altos índices de ruídos produzidos pelas máquinas de processamento e beneficiamento. Percebe-se, portanto, a importância da ergonomia, num contexto global, para melhoria da qualidade de vida dos trabalhadores, com ênfase nos aspectos físicos, cognitivos e organizacionais do trabalho.

Uue "Shreki" hääled ikka veel leidmata / Helen Teesalu

Index Scriptorium Estoniae

Teesalu, Helen

2007-01-01

Janno Põldmal seisab ees ameerika animafilmi "Shrek 3" dubleerimine eesti keelde. Eesti esilinastus peaks olema 8. juunil. Näitlejate häälte osadesse valimisele tootja DreamWorks Animation poolt esitatud nõuetest. Lisatud nimekiri : tegelaste hääli esitavad näitlejad originaalversioonis ja oletatavalt eestikeelses versioonis

Ärimudelid Ameerika (ja Eesti) kvaliteetajalehtede päästmiseks / Mikk Salu

Index Scriptorium Estoniae

Salu, Mikk, 1975-

2009-01-01

CNN-i endine juht Walter Isaacson pakkus välja mikromaksete süsteemi, kus ajalehtede online-versiooni sisu hakatakse müüma artikli kaupa. Yale'i ülikooli finantsjuhi David Swenseni arvates võiksid kvaliteetajalehed kopeerida ülikoolides kasutatavat ärimudelit - mittetulundusühingutest tuleks muutuda investeerimisfondideks

Efeito da safra vitícola na composição da uva, do mosto e do vinho Isabel da Serra Gaúcha, Brasil

OpenAIRE

Rizzon,Luiz Antenor; Miele,Alberto

2006-01-01

Entre os fatores que interferem na composição e na qualidade do vinho, destaca-se a safra vitícola, com suas variações climáticas. Com este objetivo, avaliou-se o efeito das safras de 1999, 2000 e 2001 na composição da uva, do mosto e do vinho Isabel da Serra Gaúcha. Nesse sentido, foram selecionados vinte e dois produtores de uva Isabel de onze municípios dessa região. Por ocasião da maturação, para as avaliações da uva e do mosto e a elaboração das microvinificações, foram colhidos 60kg de ...

Assessment of water and seafood microbiology quality in a mangrove region in Vitória, Brazil.

Science.gov (United States)

Keller, Regina; Justino, Juliana F; Cassini, Sérvio Túlio

2013-09-01

Mangroves are vital part of the local economy for some communities in the region of Vitória, Brazil. Oysters, mussels, and crabs, which are naturally abundant in the mangroves, are harvested and largely consumed in restaurants and by the population. In recent years, unusually high rates of annual gastroenteritis cases have been reported in the region suggesting an association between the consumption of contaminated shellfish and the development of gastrointestinal diseases. The objective of this study was to evaluate water samples and mussels collected in the mangrove region using bacterial indicator Escherichia coli and enteric viruses (adenovirus, rotavirus, and norovirus). Our results showed that the region of study is impacted by a continuous discharge of domestic sewage. Although E. coli was detected at low densities in water samples, mussels were shown to be 400 times more contaminated throughout the period of the study. Adenovirus and rotavirus genomes were detected by nested-polymerase chain reaction respectively in 76 and 88% of water samples and 100% of mussel samples. Norovirus was found in 4.8% of water samples and was not detected in the mussels. The screening of bivalves for the presence of health-significant enteric viruses can help in the prevention of outbreaks among shellfish consumers and contribute to improvement of the estuarine environment.

Tänavune provintsiteatripäev soovis Koidulaulikule õnne sünnipäevaks / Ene Kallas

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Kallas, Ene

2008-01-01

Muljeid Saaremaa 11. provintsiteatripäevalt, mis pühendatud Lydia Koidula 165. sünniaastapäevale. Kuus näiteseltskonda esitas Koidula näidendid "Kosjakased", "Säärane mulk ehk Sada vakka tangusoola", "Saaremaa onupoeg" ja Tõnis Kipperi versiooni näidendist "Kosjaviinad" ning luulekompositsiooni "Mu isamaa on minu arm"

Skype otsib endale uusi kliente ettevõtete seast / Lauri Linnamäe

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Linnamäe, Lauri

2007-01-01

Ilmunud ka: Postimees : na russkom jazõke, 30. juuli 2007, lk. 5. Kuna 30 protsenti Skype'i klientidest kasutab suhtlustarkvara tööasjus, kavatseb firma tegeleda äriklientide juurdevõitmisega, tuues müügile ettevõtetele mõeldud Skype'i versiooni Skype for Business. Lisa: Skype'i tähelend

A 44 bp intestine-specific hermaphrodite-specific enhancer from the C. elegans vit-2 vitellogenin gene is directly regulated by ELT-2, MAB-3, FKH-9 and DAF-16 and indirectly regulated by the germline, by daf-2/insulin signaling and by the TGF-β/Sma/Mab pathway.

Science.gov (United States)

Goszczynski, Barbara; Captan, Vasile V; Danielson, Alicia M; Lancaster, Brett R; McGhee, James D

2016-05-01

The Caenorhabditis elegans vitellogenin genes are transcribed in the intestine of adult hermaphrodites but not of males. A 44-bp region from the vit-2 gene promoter is able largely to reconstitute this tissue-, stage- and sex-specific-expression. This "enhancer" contains a binding site for the DM-domain factor MAB-3, the male-specific repressor of vitellogenesis, as well as an activator site that we show is the direct target of the intestinal GATA factor ELT-2. We further show that the enhancer is directly activated by the winged-helix/forkhead-factor FKH-9, (whose gene has been shown by others to be a direct target of DAF-16), by an unknown activator binding to the MAB-3 site, and by the full C. elegans TGF-β/Sma/Mab pathway acting within the intestine. The vit-2 gene has been shown by others to be repressed by the daf-2/daf-16 insulin signaling pathway, which so strongly influences aging and longevity in C. elegans. We show that the activity of the 44 bp vit-2 enhancer is abolished by loss of daf-2 but is restored by simultaneous loss of daf-16. DAF-2 acts from outside of the intestine but DAF-16 acts both from outside of the intestine and from within the intestine where it binds directly to the same non-canonical target site that interacts with FKH-9. Activity of the 44 bp vit-2 enhancer is also inhibited by loss of the germline, in a manner that is only weakly influenced by DAF-16 but that is strongly influenced by KRI-1, a key downstream effector in the pathway by which germline loss increases C. elegans lifespan. The complex behavior of this enhancer presumably allows vitellogenin gene transcription to adjust to demands of body size, germline proliferation and nutritional state but we suggest that the apparent involvement of this enhancer in aging and longevity "pathways" could be incidental. Copyright © 2016 Elsevier Inc. All rights reserved.

Seleção de bactérias promotoras de crescimento no abacaxizeiro cultivar Vitória durante a aclimatização Selection of growth-promoting bacteria for pineapple 'Vitória' during acclimatization

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Lílian Estrela Borges Baldotto

2010-04-01

Full Text Available A propagação in vitro do abacaxizeiro (Ananas comosus L. Merril resulta na produção de uma grande quantidade de mudas sadias e homogêneas. Apesar dessas vantagens, a necessidade de um longo período de aclimatização onera essa prática agrícola. A aceleração do crescimento das plantas pela inoculação de bactérias diazotróficas endofíticas e epifíticas pode ser útil para diminuir esse período. O objetivo deste trabalho foi avaliar o potencial de 20 estirpes de bactérias diazotróficas em sintetizar indol, solubilizar fosfato de Ca e óxido de Zn e atuar antagonicamente ao fungo fitopatogênico Fusarium subglutinans f. sp. ananas, bem como, posteriormente, avaliar o desempenho do abacaxizeiro 'Vitória' propagado por cultura de tecidos em resposta à inoculação bacteriana durante o período de aclimatização em casa de vegetação. Foram medidas as características de crescimento da parte aérea e do sistema radicular e o conteúdo de nutrientes de folhas do abacaxizeiro. Os resultados mostraram diferenças na capacidade das bactérias de sintetizar indol, solubilizar fosfato de Ca e óxido de Zn e atuar antagonicamente ao Fusarium. Foram também constatadas diferenças na capacidade das bactérias em promover o crescimento da parte aérea e do sistema radicular e o acúmulo de N, P, K, Ca e Mg em folhas do abacaxizeiro. A inoculação das bactérias diazotróficas selecionadas pode promover o crescimento das mudas durante o período de aclimatização, melhorando a adaptação do abacaxizeiro ao ambiente ex vitroPineapple (Ananas comosus L. Merrill in vitro propagation results in the production of a large quantity of healthy and homogeneous plantlets. Despite these advantages, the long time required for acclimatization makes this agricultural practice too costly. Growth acceleration of plantlets by inoculation with endophytic and epiphytic diazotrophic bacteria may be useful to reduce this period. The objectives of this

"West Side'is" kõlab lõpuks Natalie Woodi hääl

Index Scriptorium Estoniae

2003-01-01

Ameerika näitlejanna Natalie Wood (1938-1981), kes mängis 1961.a. ekraanile jõudnud muusikalis "West Side Story" Mariat, laulis küll oma partii linti, kuid ekraanil kõlas Mariana laulmas Mansi Nixoni hääl. 2002.a. välja lastud DVD versioonis laulab laulu "I Feel Pretty" Natalie

Faz-de-conta que eu brinco : o comparecimento da brincadeira na educação infantil da rede pública de Vitória da Conquista-Bahia

OpenAIRE

Silva, Carmem Virgínia Moraes da

2007-01-01

Esta pesquisa tem como objetivo compreender o lugar da brincadeira na educação infantil da rede pública de Vitória da Conquista Bahia. Entendemos a instituição de educação infantil como espaço que deve respeitar a criança como pessoa em condição peculiar de desenvolvimento. Adotamos a abordagem sócio-histórica compreendida a partir de Vygotsky e Leontiev, com a teoria social da construção do conhecimento e seu diálogo com autores como Piaget e Brougère. A brincadeira é vista como fruto das ...

Revisão sistemática sobre a epidemiologia das doenças cardiovasculares e respiratórias e suas associações com a poluição do ar em Vitória/ES

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Sergio Tufik

2017-07-01

Full Text Available Introdução: A poluição do ar é um importante fator ambiental relacionado a doenças crônicas não transmissíveis, como doenças cardiovasculares e respiratórias. Essa relação torna-se importante em cidades com alta atividade industrial, como Vitória/ES. Esta pesquisa utilizou três revisões sistemáticas (RSs distintas para avaliar a epidemiologia das doenças cardiovasculares (RS1, a epidemiologia das doenças respiratórias (RS2 e a associação entre poluição do ar e doenças cardiorrespiratórias (RS3 na cidade de Vitória. Métodos: A busca bibliográfica considerou três bases de dados (PubMed, Scopus e LILACS. Foi estabelecida uma quantidade mínima de três estudos para a condução de metanálises. A heterogeneidade de cada análise foi calculada pelo índice I2 . Resultados: A busca bibliográfica resultou em 1.205 registros, dos quais 27 foram incluídos – 17 na RS1, cinco na RS2 e cinco na RS3. Apenas dois desfechos puderam ser avaliados por meio de metanálises: prevalência de hipertensão e prevalência de asma em crianças. Estima-se uma prevalência de 34% (IC95%: 24-45 de hipertensão e de 12% (IC95%: 1-32 de asma em crianças. Os poluentes mais avaliados foram MP10 e SO2 , para os quais não se pôde realizar metanálises. Todas as análises apresentaram baixo número de registros incluídos e alta heterogeneidade. Conclusão: Há poucos dados disponíveis sobre a associação de doenças cardiorrespiratórias e poluição ambiental em Vitória. A heterogeneidade, a diversidade de desfechos e a baixa quantidade de estudos impediram a condução de análises mais profundas e limitaram o poder de síntese e conclusão dessas revisões, impossibilitando uma avaliação adequada dos objetivos propostos. São necessários mais estudos epidemiológicos com amostras de grande porte e representativas para que se tenha resultados conclusivos sobre a relação entre poluição ambiental e doenças cardiorrespirat

Avaliação da sustentabilidade em canteiros de obras: um estudo na Grande Vitória - ES

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Prisicla do Nascimento

2016-12-01

Full Text Available Buscando identificar o quão sustentável um canteiro de obras pode ser, este estudo vem propor uma metodologia de avaliação da sustentabilidade com base em boas práticas adotadas pelos selos de certificações ambientais existentes. A avaliação foi realizada por meio de um questionário com cinco temáticas: geração de resíduos, consumo de água, consumo de energia, relação com o entorno e prevenção da poluição. Como estudo de caso foi apresentado uma avaliação de como está sendo absorvido o conceito de sustentabilidade em canteiros de obras de quinze construtoras da Grande Vitória – ES, traçando-se assim um panorama das práticas adotadas nos respectivos canteiros de obras. Numa etapa final foram compilados os resultados das visitas, e a partir da exposição dos dados analisados foi possível constatar o nível de práticas sustentáveis abordadas durante a execução das obras.

Patrones de manejo y negociación por el agua en parajes tsotsiles de la ladera sur del volcán Tsonte’vits, Chiapas, México

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Daniel Murillo Licea

2017-01-01

Full Text Available Se identifican los elementos que aparecen en los patrones de manejo y negociación del agua por población tsotsil de algunos parajes de varios municipios situados en la parte sur del volcán Tsonte’vits, en Los Altos de Chiapas, México. Mediante trabajo de campo directo, recorridos por varios manantiales, observación participante y entrevistas se analizan los sistemas de uso, aprovechamiento y negociación entre parajes para otorgar agua para uso doméstico. Dichos patrones de manejo del agua obedecen a reglas consuetudinarias, a negociaciones interparaje, y a un sentido de identidad y de territorialidad tsotsil entre los municipios de la región estudiada.

Desdobramentos da educação permanente em saúde no município de Vitória, Espírito santo Ramifications of continuing education in health in Vitória, state of Espírito Santo

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Elzimar Evangelista Peixoto Pinto

2010-06-01

Full Text Available Este artigo tem como objetivo discutir os desdobramentos do processo de formação de facilitadores e o trabalho de efetivação de 'rodas' de Educação Permanente em Saúde (EPS em unidades de serviços de saúde no município de Vitória, Espírito Santo, cenário selecionado a partir de mapeamento dentre várias experiências de EPS em andamento no país. Adota os pressupostos metodológicos sugeridos pela cartografia para produção e análise dos dados, utilizando variadas estratégias, como observação participante, entrevistas individuais e coletivas com os profissionais e usuários. Verifica que o termo EPS admite várias concepções: movimento que coloca o trabalho em análise, visando a definir prioridades locais que promovam a reorganização dos espaços de produção de saúde; estratégia de formação dos profissionais de saúde em um processo constante de subjetivação, como estratégia de gestão, na medida em que possibilita reorganizar a gestão a partir da problematização do trabalho. Verifica a existência de 59 Rodas de EPS distribuídas em 25 serviços de saúde, com a presença de dois facilitadores em cada uma. Conclui que, nesse cenário, as várias concepções disputaram espaço e produziram efeitos, especialmente perceptíveis na mudança da forma como os profissionais compreendem o processo educativo e a sua interação com o cotidiano do trabalho.This article aims to discuss the ramifications of the process of training of facilitators and materializing Permanent Education in Health (PEH 'rounds' in health services units in Vitória, state of Espírito Santo, the scenario which was selected to map several PEH experiments already underway in the country. It adopts the methodological assumptions suggested by mapping to produce and analyze data, using various strategies such as participant observation and individual and collective interviews with professionals and users. It was noted that the PEH expression

Fatores de risco cardiovascular em crianças de 7 a 10 anos de área urbana, Vitória, Espírito Santo, Brasil Cardiovascular risk factors in 7-to-10-year-old children in Vitória, Espírito Santo State, Brazil

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Maria del Carmen Bisi Molina

2010-05-01

Full Text Available O objetivo do estudo foi identificar a ocorrência simultânea de fatores de risco cardiovascular em crianças de 7 a 10 anos de Vitória, Espírito Santo, Brasil, e investigar variáveis socioeconômicas associadas. Foram pesquisadas 1.282 crianças e obtidos dados antropométricos e medidas da pressão arterial com aparelho automático. Dados socioeconômicos, de alimentação e de atividade física foram obtidos com base em questionário. Para avaliação da ocorrência simultânea de fatores de risco cardiovascular foi desenvolvido um índice a partir da presença de excesso de peso, pressão arterial elevada, alimentação de baixa qualidade e lazer sedentário > 4h/dia. Presença simultânea de quatro fatores de risco cardiovascular foi identificada como risco alto. Classe socioeconômica e escolaridade materna foram incluídas no modelo de regressão logística. Cerca de 34% das crianças apresentaram dois fatores de risco cardiovascular, 20% três fatores e 6,4 quatro fatores de risco cardiovascular. A escolaridade materna se manteve associada ao risco cardiovascular alto (OR: 7,36, IC95%: 2,09-25,97 e médio (OR: 2,57, IC95%: 1,58-4,20. Baixa escolaridade materna foi o fator mais importante associado ao risco cardiovascular.The study aimed to identify the simultaneous occurrence of cardiovascular risk factors in children 7 to 10 years of age in Vitória, Espírito Santo State, Brazil, and investigate associated socioeconomic variables. In a sample of 1,282 children, anthropometric data were obtained and blood pressure was measured with an automatic device. Socioeconomic, nutritional, and physical activity data were obtained with a questionnaire. To evaluate the simultaneous occurrence of cardiovascular risk factors, an index was developed including overweight, high blood pressure, poor eating, and sedentary leisure > 4hours/day. The simultaneous presence of four cardiovascular risk factors was defined as high risk. Socioeconomic status

Uso popular de plantas medicinais por mulheres da comunidade quilombola de Furadinho em Vitória da Conquista, Bahia, Brasil

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L. R. Oliveira

2015-10-01

Full Text Available Objetivou-se com este trabalho realizar um levantamento do uso popular de plantas medicinais por mulheres da Comunidade Quilombola de Furadinho em Vitória da Conquista-Bahia. O trabalho foi realizado na comunidade Quilombola de Furadinho, visando identificar as principais plantas utilizadas com fins medicinais, as formas de obtenção, sua indicação medicinal, a parte da planta utilizada, as formas de preparo, bem como as formas de obtenção e transmissão deste conhecimento. Para a realização dessa pesquisa foram entrevistadas 14mulheres com idades variadas, moradoras dessa comunidade, através de questionários semi estruturados, e análise descritiva. Constatou-se um vasto conhecimento relacionado ao uso de plantas medicinais entre as entrevistadas, o qual foi adquirido de forma oral através de pais e avós. As plantas medicinais utilizadas são em sua maioria cultivadas em quintais, sendo as folhas a parte mais utilizada nas preparações medicamentosas, e a principal forma de preparo são os chás. Foram identificadas 30 plantas com fins medicinais e terapêuticos, sendo a Erva cidreira, o Capim santo, o Hortelã grosso, a Arruda e a Erva doce as mais utilizadas.Popularized use of medicinal plants by women of Quilombo of Furadinho community in Vitoria da Conquista, Bahia, Brazil Abstract: This work aimed to carry out a survey of the popular use of medicinal plants by women of Furadinho Quilombo Community in Vitória da Conquista, Bahia. The work was conducted in the Furadinho Quilombo Community, aiming at identifying the main plants used for medical purposes, the ways of obtaining, its indication medicinal, the part of the plant used, the forms of preparation, as well as ways of obtaining and transmission of this knowledge. For the realization of this research were interviewed 14 women with ages varied, dwell in this community, through semi-structured questionnaires, and descriptive analysis. It was found a vast knowledge related to

Acute liver failure in children: observations in Vitória, Espírito Santo State, Brazil Insuficiência hepática aguda na criança: observações em Vitória, Estado do Espírito Santo, Brasil

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Sandra F. Moreira-Silva

2002-10-01

Full Text Available In this communication we report 46 cases of acute liver failure in children diagnosed at the Hospital Infantil Nossa Senhora da Glória in Vitória, E Santo. Serology for IgM anti-HAV, IgM anti-HBc, HbsAg, anti-HCV and biochemical tests were performed in all cases in a routine laboratory. The M/F ratio was 1.1:1 and the mean age was 4.7±3.2 years, without gender difference. Anti-HAV IgM+ in 38 (82.6% cases, anti-HbcIgM+ in two (4.3 % cases and 6 (13.1% cases were negative for all viral markers investigated. Anti- HCV+ in one anti-HAV IgM+ case. HbsAg+ in two anti-HbcIgM+ and in two HAVIgM+ cases. Among the six A, B and C negative cases, four (8.6% did not have the suspected exogenous intoxication. Mortality was 50%, without gender or age differences. These results demonstrate that HAV infection is the main etiology of acute liver failure in children in Brazil, confirming that, although it is a self limited, relatively mild illness, it can cause serious and even fatal disease. The observation of four cases without A, B and C viral markers and no history of exogenous intoxication, agree with the observation of non A-E acute sporadic hepatitis in Northeastern Brazil.São relatados 46 casos de insuficiência hepática aguda, diagnosticados no Hospital Infantil Nossa Senhora da Glória, em Vitória, Espírito Santo. Sorologia para IgM anti-HAV, IgM anti-HBc, HbsAg, anti-VHC e testes bioquímicos realizados em laboratório de rotina. Relação M/F de 1,1:1; média de idades: 4,7±3,2 anos, sem diferença entre os sexos. IgM anti-VHA+ em 38 (82.6% casos, IgM anti-HBc+ em dois (4,3% casos e seis (13,1%casos foram negativos para os marcadores virais investigados. HbsAg+ em dois casos IgM anti-HBc+ e em dois com IgM anti-VHA+. Anti-VHC+ em um caso IgM anti-VHA+. Entre os seis casos negativos para VHA, VHB e VHC, quatro (8,6% não tinham suspeita de intoxicação exógena. A mortalidade foi de 50% sem diferenças em relação à idade ou sexo. Os

A situação da lei na ótica da subjetividade: um estudo no vivido das crianças e jovens na Casa-Lar, Bela Vista - Vitória ES

OpenAIRE

Oliveira, Arlete Correa de

2011-01-01

Este trabalho questiona a lei, sob a ótica da subjetividade, no universo de crianças e jovens abrigados na Casa-Lar Bela Vista, Vitória, Espírito Santo. Sua temática central é a lei da proibição do incesto (EEP) e os complexos familiares (Lacan) cujos pressupostos teóricos orientam esta pesquisa e permitem explicitar a presença dessa lei na subjetividade humana e no laço social. Dentro desse questionamento, surge a importância da interface entre a assistência social e a explici...

Avaliação de interações medicamentosas potenciais em prescrições da atenção primária de Vitória da Conquista (BA), Brasil

OpenAIRE

Leão,Danyllo Fábio Lessa; Moura,Cristiano Soares de; Medeiros,Danielle Souto de

2014-01-01

As interações medicamentosas são fatores de risco para a ocorrência de reações adversas a medicamentos. Este estudo teve o objetivo de avaliar as interações medicamentosas potenciais em prescrições da atenção primária de Vitória da Conquista (BA), visando preencher a lacuna de conhecimento sobre essa temática no Brasil. Foram coletadas informações sobre diversas variáveis de prescrições oriundas da atenção primária e as interações medicamentosas avaliadas a partir dos bancos de dados do Medsc...

Behavior assessment of women attending a sexually transmitted disease clinic in Vitória, Brazil Práticas comportamentais em mulheres atendidas em clínica de doenças sexualmente transmissíveis em Vitória, Brasil

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Angelica Espinosa Miranda

2012-04-01

Full Text Available BACKGROUND: Studies about sexual risk behaviors can provide information to support design strategies to control the spread of HIV infection. OBJECTIVE: To assess sexual risk behaviors among women attending a sexually transmitted diseases clinic in Vitória, Brazil. METHODS: A cross-sectional study was performed among women attending an STD/AIDS reference center. Enrolled participants were interviewed and provided a blood sample to determine HIV status. RESULTS: A total of 276 women participated. among 284 selected; 109 (39.5% were HIV-positive and 167 (60.5% HIV-negative. Median age was 31 years (interquartile range (IQR24-36 and 69% of women were between 18 and 34 years of age. Women reported high access to information about STD (87% and AIDS (90% but information about sexuality was less common (55%. HIV-positive women asked their partners to use condoms more often than HIV-negatives (31% vs. 5%, p=0.02, and were more likely to have used a condom at last intercourse (65% vs. 33%, pFUNDAMENTOS: Estudos sobre comportamentos sexuais de risco fornecem informações para programar estratégias para o controle da expansão da infecção pelo HIV/AIDS. OBJETIVO: Avaliar os comportamentos de risco sexual entre mulheres atendidas em clínica de doenças sexualmente transmissíveis em Vitória, Brazil. MÉTODOS: Estudo de corte-transversal foi realizado com mulheres atendidas no Centro de Referência para DST/Aids. As pacientes selecionadas foram entrevistadas e autorizaram a coleta de uma amostra de sangue para determinar sorologia para HIV. RESULTADOS: Um total de 276 mulheres participou, entre as 284 selecionadas; 109 (39,5% eram HIV-positivas e 167 (60,5% HIV-negativas. A mediana de idade foi 31 anos (distância interquartil 24-36 e 69% das mulheres tinham entre 18 e 34 anos de idade. As mulheres relataram alto grau de acesso a informações sobre doenças sexualmente transmissíveis (87% e Aids (90%, mas as informações sobre saúde sexual foram

Contemporary Dances of Death in Niguliste Museum / Triin Hallas

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Hallas, Triin, 1987-

2009-01-01

Bernt Notke 500. surma-aastapäevaga seoses Niguliste Muuseumis toimunud õpilaste haridusprogrammi raames valmisid tööd, mis esitavad tänapäeva versiooni Bernt Notke maalist "Surmatants". Õpilaste tööd olid 2009. a. väljas näitusel "Tänapäeva surmatants" ja Anu Männi kureeritud näitusel "Bernt Notke - uuenduste ja traditsioonide vahel"

Vitamin E and correlated antioxidants: A gamma radiolysis study

International Nuclear Information System (INIS)

Jore, D.; Kaouadji, M.N.; Ferradini, C.

1990-01-01

Gamma irradiations of Vit.E-Vit.C aerated ethanolic solutions have been performed for several ratios (Vit.E)/(Vit.C) between 0.1 and 50. The obtained results show that Vit.C is able to regenerate Vit.E from its oxidized radical, this regeneration being total for a ratio (Vit.E)/(Vit.C) greater than or equal to 27 in our conditions of irradiation. The ratio (Vit.E)/(Vit.C) seems to be the main factor of this synergestic effect towards peroxyl radicals scavenging

Chemical profile of pineapple cv. Vitória in different maturation stages using electrospray ionization mass spectrometry.

Science.gov (United States)

Ogawa, Elizângela M; Costa, Helber B; Ventura, José A; Caetano, Luiz Cs; Pinto, Fernanda E; Oliveira, Bruno G; Barroso, Maria Eduarda S; Scherer, Rodrigo; Endringer, Denise C; Romão, Wanderson

2018-02-01

Pineapple is the fruit of Ananas comosus var. comosus plant, being cultivated in tropical areas and has high energy content and nutritional value. Herein, 30 samples of pineapple cv. Vitória were analyzed as a function of the maturation stage (0-5) and their physico-chemical parameters monitored. In addition, negative-ion mode electrospray ionization mass spectrometry [ESI(-)FT-ICR MS] was used to identify and semi-quantify primary and secondary metabolites present in the crude and phenolic extracts of pineapple, respectively. Physico-chemical tests show an increase in the total soluble solids (TSS) values and in the TSS/total titratable acidity ratio as a function of the maturity stage, where a maximum value was observed in stage 3 (¾ of the fruit is yellow, which corresponds to the color of the fruit peel). ESI(-)FT-ICR MS analysis for crude extracts showed the presence mainly of sugars as primary metabolites present in deprotonated molecule form ([M - H] - and [2 M - H] - ions) whereas, for phenolic fractions, 11 compounds were detected, being the most abundant in the third stage of maturation. This behavior was confirmed by quantitative analysis of total polyphenols. ESI-FT-ICR MS was efficient in identifying primary (carbohydrates and organic acids) and secondary metabolites (13 phenolic compounds) presents in the crude and phenolic extract of the samples, respectively. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

Abandonment of antiretroviral therapy among HIV-positive patients attended at the reference center for HIV/AIDS in Vitória, Brazil.

Science.gov (United States)

Zago, Adriana Marchon; Morelato, Paola; Endringer, Emmanuele de Angeli; Dan, Germano de Freitas; Ribeiro, Evanira Mendes; Miranda, Angelica Espinosa

2012-01-01

This study evaluates the risk factors for the abandonment of antiretroviral therapy (ART) among patients receiving care in an AIDS clinic in Vitória, Brazil. We conducted a case-control study of patients with AIDS attending a reference center for sexually transmitted disease (STD)/AIDS. A total of 62 patients, who abandoned therapy in 2008, and 188 HIV-infected patients answered an interview including demographic, social, and clinical characteristics. Risk factors associated with abandon in univariate analysis were entered into logistic regression models. A total of 250 patients were included in the study. Groups were similar regarding age, gender, and monthly income. In the final multivariate model, illicit drug use (adjusted odds ratio [AOR], 2.3; 95% confidence interval [CI], 1.03-5.07), previous abandon of medication (AOR 38.6; 95% CI 10.49-142.25), last CD4 count <200 cells/mm(3) (AOR 1.5; 95% CI 1.03-2.10), and viral load higher than 1000 copies/mL (AOR 2.0 (95% CI 1.34-3.09) were independent predictors of abandonment of ART. In addition to the clinical indicators, behavioral factors remained important throughout the multivariate analysis in our study.

Reactional state and nutritional profile among leprosy patients in the primary health care system, Greater Vitória, Espírito Santo State, Brazil Estado reacional e perfil nutricional em portadores de hanseníase acompanhados na rede de atenção primária à saúde da Grande Vitória, Espírito Santo, Brasil

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Rosa Maria Natalli Montenegro

2012-01-01

Full Text Available Leprosy may present acute/subacute inflammatory processes (leprosy reactions. The study characterized the reactional states of patients at health clinics in Vitória, Espírito Santo State, Brazil, and associated them with sociodemographic factors and clinical/nutritional variables. between January and December 2009, longitudinal follow-up of patients with leprosy continued until leprosy reactions occurred or patients completed 6 months of multidrug therapy. Of the 151 patients participating, 78 (51.7% were females, 48 (31.8% had 5 to 8 years schooling, 93 (61.6% worked and earned from 1 to 3 minimum wages, and 55 (36.4 % had leprosy reactions, but with no statistical association to socioeconomic characteristics or nutritional status. However, absence of reaction was more common in the low-weight group, suggesting a trend in this group to protection from the reaction (p = 0.0906. The study found no association between nutritional status and leprosy reaction.A hanseníase pode apresentar processos inflamatórios agudos/subagudos (reações hansênicas. Os objetivos foram caracterizar os estados reacionais de pacientes de Unidades de Saúde da Grande Vitória (Espírito Santo, Brasil e associá-los aos fatores sociodemográficos e a variáveis clínicas/nutricionais. Estudo longitudinal, de monitoramento de portadores de hanseníase acompanhados até o aparecimento da reação hansênica ou até 6 meses iniciais da poliquimioterapia, de janeiro a dezembro de 2009. Participaram 151 pacientes, sendo 78 (51,7% femininos; 48 (31,8% estudaram de cinco a oito anos; 93 (61,6% trabalhavam e ganhavam de um a três salários mínimos; 55 (36,4% apresentaram reação hansênica, sem associação estatística às características socioeconômicas nem ao estado nutricional. Porém, a ausência de reação foi maior no grupo baixo peso, sugerindo neste grupo tendência na proteção da reação (p = 0,0906. No estudo não houve associação do estado

Revitalização de centros urbanos no Brasil: uma análise comparativa das experiências de Vitória, Fortaleza e São Luís

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Tarcísio R. Botelho

2005-08-01

Full Text Available O estudo dos processos de revitalização urbana tem ocupado um importante lugar na compreensão da dinâmica urbana contemporânea dada a freqüência e a abrangência que eles têm conhecido. Nas últimas décadas, estes processos têm se caracterizado por incorporarem a “cultura” como conteúdo diferenciador das várias experiências de revitalização. Neste artigo, avalio o lugar do patrimônio cultural nas experiências recentes de revitalização dos centros de Vitória (ES, de Fortaleza (CE e de São Luís (MA, enfocando sobretudo o papel do poder público para obter êxito na implantação das políticas de revitalização. Considero que os processos de revitalização aqui enfocados apontam para articulações inovadoras entre os atores envolvidos, na medida em que atingem conjuntos urbanos históricos diferentemente valorizados e passam por governos locais diferentemente comprometidos com os processos de revitalização dos centros urbanosThe study of urban revitalization processes has taken pride of place in the studies of contemporary urban dynamics, given their frequency and broad scope. In the last few decades, they have stood out, among a variety of revitalization processes, for their ability to incorporate “culture” as a differentiating content. In this article, I evaluate the part the heritage has played in the latest attempts at revitalizing the historical areas of Vitória (ES, Fortaleza (CE and São Luís (MA, placing special emphasis on the role of the public power to enforce successful revitalization policies. In my opinion, the revitalization processes included in this paper point to innovative articulations among the players involved in them, once they comprise urban historical complexes with items of different values and are subject to local governments more or less committed to the revitalization of urban centers

Venemaa haridusministeerium kui müüt ja möödapääsmatus : haridustemaatikast eestlaste 1864. ja 1881. aasta suurmärgukirjades / Väino Sirk

Index Scriptorium Estoniae

Sirk, Väino, 1942-

2016-01-01

Märgukirja saksakeelses kavandis räägitakse koolide allutamisest kroonule. Eestikeelses tekstis on tajutav lootus riigi ainelisele abile. Venekeelses versioonis on rõhutatud halduslik, valitsuslik aspekt. 1881. aasta märgukirja erinevusest 1864. aasta märgukirjast. Teine suurmärgukiri esitati venestamise eelõhtul. Haridust käsitlev punkt kajastas eriarvamusi eestlaste eneste keskel, veelgi enam aga eesti ühiskonna ootuste ja Vene poliitika vahelist varjatud pinget

A educação especial no âmbito do planejamento orçamentário do município de Vitória, Espírito Santo

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Marileide Gonçalves França

2016-06-01

Full Text Available Este texto analisa a ação do Poder Público no provimento de recursos à educação especial no âmbito do planejamento orçamentário do município de Vitória, Espírito Santo, no período de 2008 a 2013. Entende o Estado como espaço público marcado pela correlação de forças entre classes que compõem a sociedade e participam da formulação de políticas públicas. Para tanto, realiza pesquisa documental e evidencia que a educação especial integrava o orçamento municipal. Contudo, os valores irrisórios previstos para essa modalidade estavam subdimensionados e/ou agregados às outras etapas de ensino.

Informação e Desenvolvimento Rural: o caso de produtores rurais assentados em Vitória de Santo Antão (PE

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Leonardo Rodrigues Ferreira

2010-01-01

Full Text Available This research aims to investigate how information is transmited in family farms, and how the use of information can help to improve agricultural productivity, specially with technical improvements in cassava production in rural areas, such as Vitória de Santo Antão / PE, in order to identify the constraints in the transmission of information reported by rural workers, as much as present rural workers¿ profile and evaluate the competitive advantage of the use of information for productivity of cassava based on productivity indicators. The analyses covered a sample of 15 producers in a universe of 24 farmers participating in the project coordinated by SNE. Questionnaires were addressed to workers and technicals, as well as the technique of systematic observation in order to know the methodology used in technical areas. The results revealed that the limiting process of transmitting information to farmers are due mainly to the poor education of farmers, the lack of appropriate technology due to limited resources, the resistance to changings and lack of human and financial assistance for the training of producers.

Cartografando estratégias de resistência construídas por educadores no cotidiano de trabalho numa escola de Vitória-ES

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Maria Elizabeth Barros de Barros

2008-12-01

Full Text Available Este estudo buscou cartografar as estratégias de resistência construídas por professores de uma escola pública municipal de ensino infantil da cidade de Vitória, considerando a dinamicidade da produção de saúde e doença. A cartografia se efetivou por meio de vivência institucional, entrevistas, construção de um diário de campo, questionários, fotografias e confrontação-validação dos resultados com o coletivo de trabalhadores. Apesar de todas as nocividades do ambiente de trabalho, os professores não se deixam paralisar e criam estratégias, novas formas de fazer seu trabalho, buscando condições menos adoecedoras para o desenvolvimento de suas atividades que, quando coletivizadas, potencializam o processo inventivo desses trabalhadores. Os resultados da pesquisa indicam a importância dessas discussões, uma vez que dão visibilidade a essas formas de luta, o que pode promover transformação do que é vivido nas escolas hoje.

Biochemical studies of the differentiation of HL-60 cells into monocytes by either IFN, VIT, D3 or TPA

International Nuclear Information System (INIS)

Miyamoto, S.; Whyzmuzis, C.; Oronsky, B.; Wu, J.M.

1987-01-01

The authors have studied the differentiation process of the human promyelocytic cell line, HL-60, by treatment of these cells with either gamma interferon, 1, 25 dihydroxyvitamin D 3 or a phorbol ester, TPA. The cells were grown in RPMI 1640, 10% FCS with each respective agent, then pulsed labeled with 35 S-Met, harvested, lysed and subfractionated by centrifugation into post-ribosomal and ribosomal salt was fractions (RSW). These fractions were examined by SDS gel electrophoresis. The culture supernatant from the treated cells was dialyzed and passed over a heparin agarose affinity column. The absorbed material was eluted from the column by a step-wise salt gradient and analyzed by SDS gel electrophoresis. They have also observed that in a rabbit reticulocyte lysate assay, the RSW from control cells show inhibition of protein synthesis. The RSW from cells treated with either high concentrations (200-1000 units/ml) of gamma interferon, Vit D 3 or TPA did not show this inhibition. Some possible explanations for this phenomenon are the loss or inactivation of a component necessary for protein synthesis which is triggered by differentiation, or the differentiation-related modulation of translational inhibitor(s). They have used FPLC to further analyze the RSW, but because the factor(s) are present in such small quantities further analytical and more sensitive procedures need to be pursued

Structural Interaction Between GFP-Labeled Diazotrophic Endophytic Bacterium Herbaspirillum seropedicae RAM10 and Pineapple Plantlets 'VitóRia'.

Science.gov (United States)

Estrela Borges Baldotto, Lílian; Lopes Olivares, Fábio; Bressan-Smith, Ricardo

2011-01-01

The events involved in the structural interaction between the diazotrophic endophytic bacterium Herbaspirillum seropedicae, strain RAM10, labeled with green fluorescent protein, and pineapple plantlets 'Vitória' were evaluated by means of bright-field and fluorescence microscopy, combined with scanning electron microscopy for 28 days after inoculation. After 6 hours of inoculation, H. seropedicae was already adhered to the roots, colonizing mainly root hair surface and bases, followed by epidermal cell wall junctions. Bacteria adherence in the initial periods occurred mainly in the form of solitary cells and small aggregates with pleomorphic cells. Bacteria infection of root tissue occurred through the cavities caused by the disruption of epidermal cells during the emergence of lateral roots and the endophytic establishment by the colonization of intercellular spaces of the cortical parenchyma. Moreover, within 1 day after inoculation the bacteria were colonizing the shoots. In this region, the preferred sites of epiphytic colonization were epidermal cell wall junctions, peltate scutiform trichomes and non-glandular trichomes. Subsequently, the bacteria occupied the outer periclinal walls of epidermal cells and stomata. The penetration into the shoot occurred passively through stoma aperture followed by the endophytic establishment on the substomatal chambers and spread to the intercellular spaces of spongy chlorenchyma. After 21 days of inoculation, bacterial biofilm were seen at the root hair base and on epidermal cell wall surface of root and leaf, also confirming the epiphytic nature of H. seropedicae.

Structural interaction between GFP-labeled diazotrophic endophytic bacterium Herbaspirillum seropedicae RAM10 and pineapple plantlets 'Vitória'

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Lílian Estrela Borges Baldotto

2011-03-01

Full Text Available The events involved in the structural interaction between the diazotrophic endophytic bacterium Herbaspirillum seropedicae, strain RAM10, labeled with green fluorescent protein, and pineapple plantlets 'Vitória' were evaluated by means of bright-field and fluorescence microscopy, combined with scanning electron microscopy for 28 days after inoculation. After 6 hours of inoculation, H. seropedicae was already adhered to the roots, colonizing mainly root hair surface and bases, followed by epidermal cell wall junctions. Bacteria adherence in the initial periods occurred mainly in the form of solitary cells and small aggregates with pleomorphic cells. Bacteria infection of root tissue occurred through the cavities caused by the disruption of epidermal cells during the emergence of lateral roots and the endophytic establishment by the colonization of intercellular spaces of the cortical parenchyma. Moreover, within 1 day after inoculation the bacteria were colonizing the shoots. In this region, the preferred sites of epiphytic colonization were epidermal cell wall junctions, peltate scutiform trichomes and non-glandular trichomes. Subsequently, the bacteria occupied the outer periclinal walls of epidermal cells and stomata. The penetration into the shoot occurred passively through stoma aperture followed by the endophytic establishment on the substomatal chambers and spread to the intercellular spaces of spongy chlorenchyma. After 21 days of inoculation, bacterial biofilm were seen at the root hair base and on epidermal cell wall surface of root and leaf, also confirming the epiphytic nature of H. seropedicae.

Resolution of Sterile Inflammation: Role for Vitamin C

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Bassem M. Mohammed

2014-01-01

Full Text Available Introduction. Macrophage reprogramming is vital for resolution of acute inflammation. Parenteral vitamin C (VitC attenuates proinflammatory states in murine and human sepsis. However information about the mechanism by which VitC regulates resolution of inflammation is limited. Methods. To examine whether physiological levels of VitC modulate resolution of inflammation, we used transgenic mice lacking L-gulono-γ-lactone oxidase. VitC sufficient/deficient mice were subjected to a thioglycollate-elicited peritonitis model of sterile inflammation. Some VitC deficient mice received daily parenteral VitC (200 mg/kg for 3 or 5 days following thioglycollate infusion. Peritoneal macrophages harvested on day 3 or day 5 were examined for intracellular VitC levels, pro- and anti-inflammatory protein and lipid mediators, mitochondrial function, and response to lipopolysaccharide (LPS. The THP-1 cell line was used to determine the modulatory activities of VitC in activated human macrophages. Results. VitC deficiency significantly delayed resolution of inflammation and generated an exaggerated proinflammatory response to in vitro LPS stimulation. VitC sufficiency and in vivo VitC supplementation restored macrophage phenotype and function in VitC deficient mice. VitC loading of THP-1 macrophages attenuated LPS-induced proinflammatory responses. Conclusion. VitC sufficiency favorably modulates macrophage function. In vivo or in vitro VitC supplementation restores macrophage phenotype and function leading to timely resolution of inflammation.

Clinical and immunologic follow-up of patients who stop venom immunotherapy.

Science.gov (United States)

Keating, M U; Kagey-Sobotka, A; Hamilton, R G; Yunginger, J W

1991-09-01

We prospectively studied 51 self-selected Hymenoptera sting-sensitive patients to determine (1) whether a minimal or optimal duration for venom immunotherapy (VIT) exists and (2) whether clinical or immunologic parameters exist that are predictive of clinical immunity after VIT was stopped. After 2 to 10 years of VIT, all patients had deliberate sting challenges (DSCs) from live insects. If DSCs were tolerated, patients voluntarily stopped VIT and returned annually for repeat venom skin tests (VSTs) and DSCs. In most patients, it was possible to monitor VST and venom-specific antibody (Ab) levels before and after VIT was stopped. One-year after VIT, VST and venom-specific IgE and IgG Ab level results were variable; 49 patients tolerated DSC, whereas two patients exhibited generalized reactions. These two patients had pre-VIT histories of grade IV field-sting reactions and had received VIT for 2 years and 4 years, respectively. The short-term (1 year) risk of recurrence of clinical allergy to stings after VIT was higher in patients who had experienced grade IV field-sting reactions before VIT versus patients experiencing grade I to III reactions before VIT (2/15, 13% versus 0/36, 0%) and higher in patients who had received VIT for less than 5 years versus patients who received VIT for 5 or more years (2/20, 10% versus 0/31, 0%). We suggest that VIT should be continued for 5 years in patients with pre-VIT field-sting reactions of grade IV severity. VST and venom-specific Ab results do not reliably predict the outcome of DSC or the subsequent clinical course in individual patients stopping VIT.

Mudanças no Consumo e Comportamento do Consumidor a Partir da Introdução de Novos Formatos Comerciais: Um Estudo Comparativo Entre o Comércio Tradicional e Shopping Em Vitória da Conquista-Bahia-Brasil

OpenAIRE

Leite, Carlos Fernando Faria

2017-01-01

[por] A questão central desta tese doutoral é de conhecer as mudanças que aconteceram no consumo e no comportamento do consumidor tendo como unidade espacial de análise o comércio tradicional (no Centro comercial e bairro Brasil) e Shopping Conquista Sul em Vitória da Conquista-BA, correlacionado o consumo com as variáveis geográficas e socioeconômicas. Os autores lidos para subsidiar a presente tese foram Max Weber (1947), Karl Marx, Engls (1884), Keynes (1983), Bourdieu (1979), Baudrillard...

Avaliação dos teores de fluoreto na água potável distribuída pela concessionária CESAN que abastece o município de Vitória/ES

OpenAIRE

Gabriel dos Anjos de Jesus; Lindalva Marques da Silva; Tânia Maria Arrebola

2010-01-01

Este trabalho procurou avaliar os níveis de fluoreto na água fornecida pela Companhia Espírito Santense de Saneamento (CESAN), para a cidade de Vitória, capital do Estado do Espírito Santo. A adição de flúor na água tratada representa uma importante medida de saúde pública coletiva para a prevenção de cárie dental na população de uma região. A determinação do fluoreto foi realizada no Laboratório Central em Saúde Pública (LACEN) por meio do método do eletrodo de íon seletivo (ISE) indicado pe...

Imaginário e Representações Sociais a Bordo do Trem de Passageiros Vitória Minas

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Euler David De Siqueira

2011-02-01

Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4 No Brasil, a pesquisa sobre os meios de transporte, notadamente o ferroviário, enfrenta um duplo desafio: o da carência de análises que explorem a dimensão simbólica e, outro, que caminhe para além do dado mais sensível e imediato. Em outras palavras, ao invés de dados técnicos e estatísticos superficiais que inundam os livros sobre o tema do turismo, há todo um universo de representações e imaginário pronto para ser estudado. Após o longo e controvertido processo de privatização das ferrovias brasileiras, na metade dos anos 1990, o Brasil viu quase desaparecer o transporte de passageiros. A Estrada de Ferro Vitória Minas (EFVM, uma das poucas que ainda mantém em operação o transporte de passageiros, representa um espaço privilegiado à pesquisa em turismo também. A partir de um olhar sociológico e antropológico, esse artigo busca jogar luzes sobre o universo simbólico de seus passageiros. Conhecer aspectos dessa modalidade de transporte de passageiros, o que pensam, sentem e lembram seus usuários nos abre as portas ao conhecimento de suas práticas, de seu imaginário e de histórias de vida plenas de lembranças, expectativas e esquecimentos. 

Influence of Deficiency or Supplementary Selenium and a- Tochopherol (Vitamin E) In The Diet of Pubertal Male Zaraibi Goats on Fertility, Semen Quality and Testicular Traits

International Nuclear Information System (INIS)

Habeeb, A.A.M.; Teama, F.E.I.

2013-01-01

Twenty pubertal male Zaraibi goats (bucks) were randomly divided into four equal groups; fed deficient Se or vit. E, adequate Se, adequate vit. E and adequate Se + vit. E diets for 3 months to study the influence of deficient or adequate selenium (Se) and vitamin E (vit. E) in the diet of pubertal male Zaraibi goats on fertility, semen quantity and quality and some testicular traits. The results showed that the best values of semen quantity (the ejaculate volume, sperm concentration and total sperm output per ejaculate) and semen quality (percentage of progressive motility, percentage of live sperm, number of motile sperm per ejaculate, percentage of dead, abnormal spermatozoa and acrosomal abnormality) were observed in bucks fed diet supplemented with adequate Se combined with adequate vit. E. The lowest values of semen quantity and semen quality were observed in bucks suffering from deficiency of Se and/or vit. E in their diets. Testosterone level in seminal plasma was significantly higher in bucks fed adequate Se and/or vit. E than those fed diet deficient in Se and vit. E. Testosterone level was significantly higher in bucks fed diet adequate in Se + vit. E than those fed diet adequate with Se or vit. E alone. Se and vit. E deficiency in the diets was accompanied by a significant decrease in testosterone, T 4 and T 3 levels in seminal plasma. Selenium or vit. E each one alone supplementation led to increases of these hormones. T 4 and T 3 levels were significantly higher in bucks fed adequate Se or adequate Se + vit. E than in bucks fed diet with adequate vitamin E alone. Adequate Se alone and adequate Se + vit. E diets were accompanied by significant increases in Se in seminal plasma. Adequate vit. E and adequate Se + vit. E diets were accompanied by significant increase in vit. E level in the seminal plasma. It is clear that there was synergism between Se and vit. E in the biological role of Se, since the level of Se in bucks fed diet containing adequate Se

Structural Interaction Between GFP-Labeled Diazotrophic Endophytic Bacterium Herbaspirillum seropedicae RAM10 and Pineapple Plantlets ‘VitóRia’

Science.gov (United States)

Estrela Borges Baldotto, Lílian; Lopes Olivares, Fábio; Bressan-Smith, Ricardo

2011-01-01

The events involved in the structural interaction between the diazotrophic endophytic bacterium Herbaspirillum seropedicae, strain RAM10, labeled with green fluorescent protein, and pineapple plantlets ‘Vitória’ were evaluated by means of bright-field and fluorescence microscopy, combined with scanning electron microscopy for 28 days after inoculation. After 6 hours of inoculation, H. seropedicae was already adhered to the roots, colonizing mainly root hair surface and bases, followed by epidermal cell wall junctions. Bacteria adherence in the initial periods occurred mainly in the form of solitary cells and small aggregates with pleomorphic cells. Bacteria infection of root tissue occurred through the cavities caused by the disruption of epidermal cells during the emergence of lateral roots and the endophytic establishment by the colonization of intercellular spaces of the cortical parenchyma. Moreover, within 1 day after inoculation the bacteria were colonizing the shoots. In this region, the preferred sites of epiphytic colonization were epidermal cell wall junctions, peltate scutiform trichomes and non-glandular trichomes. Subsequently, the bacteria occupied the outer periclinal walls of epidermal cells and stomata. The penetration into the shoot occurred passively through stoma aperture followed by the endophytic establishment on the substomatal chambers and spread to the intercellular spaces of spongy chlorenchyma. After 21 days of inoculation, bacterial biofilm were seen at the root hair base and on epidermal cell wall surface of root and leaf, also confirming the epiphytic nature of H. seropedicae. PMID:24031612

Effects of Pulsed Electric Fields (PEF) on Vitamin C and Its Antioxidant Properties

Science.gov (United States)

Zhang, Zhi-Hong; Zeng, Xin-An; Brennan, Charles S.; Brennan, Margaret; Han, Zhong; Xiong, Xia-Yu

2015-01-01

In this study, pulsed electric fields (PEF) treatments and their effects on the structure of vitamin C (VIT-C) were estimated by fluorescence and Fourier transform infrared (FT-IR) spectroscopy, the relative content of VIT-C was measured by HPLC and the antioxidant properties of treated VIT-C by DPPH radical scavenging as well as reducing power tests. The fluorescence intensity of treated VIT-C increased slightly compared to the untreated VIT-C. Moreover, the effect of PEF on the structure of VIT-C was observed using the FT-IR spectra. These phenomena indicated that the PEF affected the conformation of VIT-C, which promoted the VIT-C isomer transformed enol-form into keto-form. In addition, the PEF treatments did not suffer the damage to VIT-C and could slow down the oxidation process in involving of experimental conditions by HPLC. The antioxidant properties of the treated VIT-C were enhanced, which was proved by radical scavenging and also the reducing power tests. PMID:26473846

Effects of Pulsed Electric Fields (PEF on Vitamin C and Its Antioxidant Properties

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Zhi-Hong Zhang

2015-10-01

Full Text Available In this study, pulsed electric fields (PEF treatments and their effects on the structure of vitamin C (VIT-C were estimated by fluorescence and Fourier transform infrared (FT-IR spectroscopy, the relative content of VIT-C was measured by HPLC and the antioxidant properties of treated VIT-C by DPPH radical scavenging as well as reducing power tests. The fluorescence intensity of treated VIT-C increased slightly compared to the untreated VIT-C. Moreover, the effect of PEF on the structure of VIT-C was observed using the FT-IR spectra. These phenomena indicated that the PEF affected the conformation of VIT-C, which promoted the VIT-C isomer transformed enol-form into keto-form. In addition, the PEF treatments did not suffer the damage to VIT-C and could slow down the oxidation process in involving of experimental conditions by HPLC. The antioxidant properties of the treated VIT-C were enhanced, which was proved by radical scavenging and also the reducing power tests.

Economia Política da Gestão de Resíduos Sólidos Municipais em Vitória (Espírito Santo

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Jérémie Cavé

2011-07-01

Full Text Available O artigo trata das interações entre a globalização dos intercâmbios econômicos e a gestão dos resíduos sólidos municipais, através do estudo de caso de Vitória-ES. Além do serviço municipal de gestão do lixo, uma diversidade de atores participa da economia da recuperação dos resíduos sólidos secos. O lixo sendo por definição aquilo que não pertence a ninguém, esses diversos atores disputam sua apropriação e conflitos surgem. Ora, a recuperação dos resíduos recicláveis é um setor de intervenção cujo caráter lucrativo é instável, dependente das variações de valor das matérias primas. A questão que se coloca, para os poderes públicos, é a de como conciliar uma cadeia de captação de resíduos sólidos ritmada pelas flutuações do mercado com um serviço publico territorial?

Chronic vitamin C deficiency promotes redox imbalance in the brain but does not alter sodium-dependent vitamin C transporter 2 expression

DEFF Research Database (Denmark)

Paidi, Maya Devi; Schjoldager, Janne Gram; Lykkesfeldt, Jens

2014-01-01

Vitamin C (VitC) has several roles in the brain acting both as a specific and non-specific antioxidant. The brain upholds a very high VitC concentration and is able to preferentially retain VitC even during deficiency. The accumulation of brain VitC levels much higher than in blood is primarily...... achieved by the sodium dependent VitC transporter (SVCT2). This study investigated the effects of chronic pre-and postnatal VitC deficiency as well as the effects of postnatal VitC repletion, on brain SVCT2 expression and markers of oxidative stress in young guinea pigs. Biochemical analyses demonstrated...... significantly decreased total VitC and an increased percentage of dehydroascorbic acid, as well as increased lipid oxidation (malondialdehyde), in the brains of VitC deficient animals (p C repleted animals were not significantly different from controls. No significant changes...

Relacionamentos Interorganizacionais: uma Análise dos Aspectos Intervenientes da Cadeia de Serviços do Setor de Comércio Exterior de Vitória (ES

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Júlia Sasso Alighieri

2009-01-01

Full Text Available The objective of the current article is to analyzethe interorganisational relationships’ enablersaspects among foreign trade services companies of Vitória (ES and its third-parties (dispatchingand transportation companies, that forms a vertical network. The main reason for this research isthe investigation of interorganisational relationships in the service sector, while the majority of thestudies with this approach focus on industrial sector. Thus, to reach such objective, the researchstrategy used was case study, using as support a qualitative methodology. Ten semi-structuredinterviews with managers of the sector had been carried out. The data was analysed throughcontent analysis and organized around themes. The empirical research allows to conclude thatinterorganisational relationships’ enablers aspectswere frequently found in the cases studied,besides evidencing the presence of another aspect not shown in the literature review: competency.As complement, we propose a model which shows the existing relations among those aspects inorder to improve the understanding of the phenomenon studied

ATIVIDADES RECREATIVAS E LÚDICAS: um estudo nas escolas estaduais da cidade de Santa Vitória do Palmar, RS – Brasil

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Alex Borges Dias

2017-11-01

Full Text Available Os processos educativos são fundamentais para a formação de cidadãos mais conscientes de seu papel na construção de uma sociedade mais justa e igualitária. Em um mundo onde as atividades humanas são constantemente aceleradas pelas práticas capitalistas, se torna cada vez mais necessário pensar o papel da educação na formação de novos cidadãos conscientes de sua importância na sociedade. Desse modo, este trabalho teve por objetivo identificar as atividades recreativas e lúdicas que contribuem nos processos educativos dos alunos do 1º ano das escolas estaduais em Santa Vitória do Palmar, RS - Brasil. Na metodologia foi feita uma pesquisa bibliográfica. Para a coleta de dados foram feitas entrevistas com os professores responsáveis pelas turmas do primeiro ano de cada escola e foi utilizado o método de observação não-participante, além do levantamento fotográfico. Identificou-se que todas as escolas estaduais da cidade possuem atividades recreativas e lúdicas que contribuem para os processos educativos dos alunos matriculados.

Análise da correlação de ocorrência da doença diarreica aguda com a qualidade da água para consumo humano no município de Vitória-ES Analysis of the correlation of diarrhea disease occurrence with drinking-water quality in the city of Vitória-ES

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Josiane Teresinha Matos de Queiroz

2009-09-01

Full Text Available Buscou-se traçar possíveis associações da qualidade da água para consumo humano com a ocorrência de casos de diarreia utilizando o Programa de Monitorização da Doença Diarreica Aguda, no ano de 2004, no município de Vitória, capital do Estado do Espírito Santo, Brasil. Para a análise da qualidade da água, foram utilizados os dados do Programa de Controle da Qualidade da Água, realizado pelo serviço de abastecimento público, e da Vigilância da Qualidade da Água, realizada pela Prefeitura local. O delineamento epidemiológico adotado foi o estudo do tipo ecológico. Foram utilizadas ferramentas de georreferenciamento para representação das amostras estudadas e análises estatísticas de regressão linear univariada e multivariada. Foram encontradas associações com significância estatística para a ocorrência de diarreia com os parâmetros de turbidez, coliformes totais e termotolerantes. Os resultados apontam para a necessidade de maior cuidado na rede de distribuição de água. Entretanto, como a doença diarreica pode ser causa de várias enfermidades, são necessárias ações com o objetivo principal de caracterizar o agravo e definir sua fonte de infecção, de modo a propor medidas que interrompam a cadeia de transmissão.Possible associations between drinking-water quality and the occurrence of diarrhea cases have been researched, using the 2004 Diarrhea Disease Monitoring Program in the city of Vitória, state of Espírito Santo, Brazil. For water quality analysis, data from the Water Quality Control Program, performed by the Public Supply Service, and from the Water Quality Surveillance, performed by the local municipal government, were used. The epidemiological delimitation adopted was the ecological study. Georeferencing tools for the representation of the studied samples and univariate and multivariate linear regression analyses were utilized. Statistically significant associations for the occurrence of

Inter-relações entre as concentrações de ozônio e de dióxido de nitrogênio na região da Grande Vitória, Espírito Santo, Brasil

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Edson Zambon Monte

Full Text Available RESUMO Este trabalho objetivou verificar as inter-relações entre as concentrações de ozônio (O3 e de dióxido de nitrogênio (NO2 na região da Grande Vitória (RGV, Espírito Santo, Brasil. Adotou-se a metodologia vetorial autorregressiva (VAR e o teste de causalidade de Granger. O modelo VAR captura as interdependências lineares entre várias séries temporais, sendo que cada variável possui uma equação estimada que representa sua evolução em termos de suas próprias defasagens e das defasagens das outras variáveis. Já o teste de causalidade de Granger baseia-se em um sistema de equações bivariado para verificar se uma variável é capaz de prever a outra. Os resultados revelaram que as concentrações de O3 e de NO2 da Região (estação de Laranjeiras foram as menos afetadas por concentrações de outras estações. Devido à localização, as concentrações de O3 e NO2 da Enseadá do Suá tiveram significativa influência de outras regiões, especialmente de Jardim Camburi, Ibes e Vitória - Centro. A concentração de ozônio na região do Ibes foi fortemente influenciada pelas concentrações de O3 e de NO2 da Enseada do Suá. Além disso, as concentrações de Cariacica sofreram impactos relevantes das concentrações da Enseada do Suá, provavelmente, devido à direção do vento Norte/Nordeste, predominante na RGV.

Growth promotion of pineapple 'vitória' by humic acids and burkholderia spp. during acclimatization Promoção do crescimento do abacaxizeiro 'vitória' por ácidos húmicos e Burkholderia spp. durante a aclimatização

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Lílian Estrela Borges Baldotto

2010-10-01

Full Text Available In vitro propagation of pineapple produces uniform and disease-free plantlets, but requires a long period of acclimatization before transplanting to the field. Quicker adaptation to the ex vitro environment and growth acceleration of pineapple plantlets are prerequisites for the production of a greater amount of vigorous, well-rooted planting material. The combination of humic acids and endophytic bacteria could be a useful technological approach to reduce the critical period of acclimatization. The aim of this study was to evaluate the initial performance of tissue-cultured pineapple variety Vitória in response to application of humic acids isolated from vermicompost and plant growth-promoting bacteria (Burkholderia spp. during greenhouse acclimatization. The basal leaf axils were treated with humic acids while roots were immersed in bacterial medium. Humic acids and bacteria application improved shoot growth (14 and 102 %, respectively, compared with the control; the effect of the combined treatment was most pronounced (147 %. Likewise, humic acids increased root growth by 50 %, bacteria by 81 % and the combined treatment by 105 %. Inoculation was found to significantly increase the accumulation of N (115 %, P (112 % and K (69 % in pineapple leaves. Pineapple growth was influenced by inoculation with Burkholderia spp., and further improved in combination with humic acids, resulting in higher shoot and root biomass as well as nutrient contents (N 132 %, P 131 %, K 80 % than in uninoculated plantlets. The stability and increased consistency of the host plant response to bacterization in the presence of humic substances indicate a promising biotechnological tool to improve growth and adaptation of pineapple plantlets to the ex vitro environment.A propagação in vitro de abacaxizeiro produz mudas uniformes e sadias, mas exige longo período de aclimatização antes da transferência para o campo. A adaptação ao ambiente ex vitro seguida da

Outsourcing de serviços públicos hospitalares na região da Grande Vitória/ES

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Marcelo Moll Brandão

2011-11-01

Full Text Available Objetiva-se com esta pesquisa analisar e apresentar a terceirização de serviços (outsourcing como estrutura de governança em hospitais públicos estaduais da Grande Vitória/ES. Utilizou-se de um estudo empírico, no qual foram realizadas pesquisas na Secretaria de Estado da Saúde, Tribunal de Contas do Estado do Espírito Santo e MinistérioPúblico do Trabalho. Foram aplicados questionários em sete hospitais, que foram respondidos pelos responsáveis dos serviços. A pesquisa foi baseada na Economia dos Custos de Transação e procurou estudar a relação de probabilidade de outsourcing com as seguintes variáveis: especificidade do ativo, frequência e incerteza. Os resultados mostraram que os riscos contratuais não são os principais direcionadores da terceirização pública hospitalar, conforme esperado, tampouco a legislação vigente, visto que, pelo baixo enforcement, verificou-se a existência de processos divergentes da lei. Observou-seainda que o administrador hospitalar gerencia pela não existência de falha em áreas críticas, abrindo mão da eficiência de custos de transação. Dessa forma, a falta de recursos por parte do Governo, a ineficiência na contratação de mão de obra pública, bem como a falta de incentivos à performance do agente público, podem levar a situações distantes na organização eficiente da atividade.

Vitamin D vitamers affect vitamin D status differently in young healthy males

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Jakobsen, Jette; Wreford Andersen, Elisabeth Anne; Christensen, Tue

2018-01-01

Dietary intake of vitamin D includes vitamin D3 (vitD3), 25-hydroxyvitamin D3 (25OH-D3), and vitamin D2 (vitD2). However, the bioactivity of the different species has not been scientifically established. The hypothesis in this study was that vitD3, 25OH-D3, and vitD2 have an equal effect on 25......-hydroxyvitamin D in serum (vitamin D status). To test our hypothesis, we performed a randomized, crossover study. Twelve young males consumed 10 µg/day vitD3 during a four-week run-in period, followed by 3 × 6 weeks of 10 µg/day vitD3, 10 µg/day 25OH-D3, and 10 µg/day vitD2. The content of vitD3, vitD2, 25OH-D3......, and 25-hydroxyvitamin D2 (25OH-D2) in serum was quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The hypothesis that the three sources of vitamin D affect vitamin D status equally was rejected. Based on the assumption that 1 µg vitD3/day will show an increase in vitamin D status...

Maternal vitamin C deficiency during pregnancy persistently impairs hippocampal neurogenesis in offspring of guinea pigs

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Tveden-Nyborg, Pernille; Vogt, Lucile; Schjoldager, Janne G

2012-01-01

While having the highest vitamin C (VitC) concentrations in the body, specific functions of VitC in the brain have only recently been acknowledged. We have shown that postnatal VitC deficiency in guinea pigs causes impairment of hippocampal memory function and leads to 30% less neurons. This study...... investigates how prenatal VitC deficiency affects postnatal hippocampal development and if any such effect can be reversed by postnatal VitC repletion. Eighty pregnant Dunkin Hartley guinea pig dams were randomized into weight stratified groups receiving High (900 mg) or Low (100 mg) VitC per kg diet. Newborn...... by stereology. Prenatal VitC deficiency resulted in a significant reduction in postnatal hippocampal volume (P...

Vitamin D deficiency among the elderly: insights from Qatar.

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Alhamad, Hanadi Khamis; Nadukkandiyil, Navas; El-Menyar, Ayman; Abdel Wahab, Luay; Sankaranarayanan, Anoop; Al Sulaiti, Essa Mubarak

2014-06-01

Vitamin D (VitD) deficiency is associated with comorbidities in the elderly. The present study investigates the prevalence of VitD deficiency among the elderly in Qatar. A retrospective study conducted between April 2010 and April 2012 that involved chart reviews. All elderly patients of age ≥65 years in geriatrics facilities including Rumailah hospital, skilled nursing facility and home healthcare services in Qatar were included in the study. Patient characteristics and outcomes were analyzed and compared according to the severity of VitD deficiency. Correlation of VitD with comorbidities was analyzed. Mean follow-up period was 6 months. A total of 889 patients were enrolled; the majority (66%) were females and the mean age was 75 ± 8.7 years. Patient comorbidities included hypertension (76.5%), diabetes mellitus (63%), dyslipidemia, (47.5%), dementia (26%) coronary artery disease (24%) and cerebrovascular accident (24%). The mean baseline serum VitD level was 24.4 ± 13.5 ng/ml; 72% of patients had VitD deficiency: mild (31%), moderate (30%) and severe (11%). Patients with severe VitD deficiency had significantly higher HbA1c levels compared with patients with optimal VitD (P = 0.03). High density lipoprotein (HDL-C) levels were significantly lower in severe VitD deficiency patients compared with optimal VitD patients (P = 0.04). There was a positive correlation between HDL-C and VitD level (r = 0.17, P = 0.001), whereas HbA1c levels showed negative correlation with VitD (r = -0.15, P = 0.009). A high prevalence of VitD deficiency (72%) was observed among the elderly in Qatar. Lower VitD was associated with higher HbA1c and lower HDL-C levels. Further studies are warranted to evaluate whether VitD supplementation controls diabetes mellitus (DM) and low HDL-C levels among the elderly.

Developmental study of vitamin C distribution in children's brainstems by immunohistochemistry.

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Coveñas, R; González-Fuentes, J; Rivas-Infante, E; Lagartos-Donate, M J; Mangas, A; Geffard, M; Arroyo-Jiménez, M M; Cebada-Sánchez, S; Insausti, R; Marcos, P

2015-09-01

Vitamin C (Vit C) is an important antioxidant, exerts powerful neuroprotective brain effects and plays a role in neuronal development and maturation. Vit C is present in brain tissue at higher concentrations than in other organs, but its detailed distribution in brain is unknown. Immunohistochemical detection of this vitamin has been performed by using a highly specific antibody against Vit C. The aim of the present work was to analyze the distribution of Vit C in children's brainstems during postnatal development, comparing two groups of ages: younger and older than one year of life. In general, the same areas showing neurons with Vit C in young cases are also immunostained at older ages. The distribution of neurons containing Vit C was broader in the brainstems of older children, suggesting that brainstem neurons maintain or even increase their ability to retain Vit C along the life span. Immunohistochemical labeling revealed only cell bodies containing this vitamin, and no immunoreactive fibers were observed. The distribution pattern of Vit C in children's brainstems suggests a possible role of Vit C in brain homeostatic regulation. In addition, the constant presence of Vit C in neurons of locus coeruleus supports the important role of Vit C in noradrenaline synthesis, which seemed to be maintained along postnatal development. Copyright © 2015 Elsevier GmbH. All rights reserved.

Inhibitory effect of vitamin C in combination with vitamin K3 on tumor growth and metastasis of Lewis lung carcinoma xenografted in C57BL/6 mice.

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Chen, Ming-Feng; Yang, Chih-Min; Su, Cheng-Ming; Liao, Jiunn-Wang; Hu, Miao-Lin

2011-01-01

Vitamin C in combination with vitamin K3 (vit CK3) has been shown to inhibit tumor growth and lung metastasis in vivo, but the mechanism of action is poorly understood. Herein, C57BL/6 mice were implanted (s.c.) with Lewis lung carcinoma (LLC) for 9 days before injection (i.p.) with low-dose (100 mg vit C/kg + 1 mg vit K3/kg), high-dose (1,000 mg vit C/kg + 10 mg vit K3/kg) vit CK3 twice a week for an additional 28 days. As expected, vit CK3 or cisplatin (6 mg/kg, as a positive control) significantly and dose-dependently inhibited tumor growth and lung metastasis in LLC-bearing mice. Vit CK3 restored the body weight of tumor-bearing mice to the level of tumor-free mice. Vit CK3 significantly decreased activities of plasma metalloproteinase (MMP)-2, -9, and urokinase plasminogen activator (uPA). In lung tissues, vit CK3 1) increased protein expression of tissue inhibitor of metalloproteinase-1 (TIMP-1), TIMP-2, nonmetastatic protein 23 homolog 1 and plasminogen activator inhibitor-1; 2) reduced protein expression of MMP-2 and MMP-9; and 3) inhibited the proliferating cell nuclear antigen (PCNA). These results demonstrate that vit CK3 inhibits primary tumor growth and exhibits antimetastastic potential in vivo through attenuated tumor invasion and proliferation.

Effects of Vitamin D Restricted Diet Administered during Perinatal and Postnatal Periods on the Penis of Wistar Rats

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Flávia Fernandes-Lima

2018-01-01

Full Text Available Vitamin D deficiency is common in pregnant women and infants. The present study aimed to investigate the effects of vitamin D restricted diet on the Wistar rats offspring penis morphology. Mother rats received either standard diet (SC or vitamin D restricted (VitD diet. At birth, offspring were divided into SC/SC (from SC mothers, fed with SC diet and VitD/VitD (from VitD mothers, fed with VitD diet. After euthanasia the penises were processed for histomorphometric analysis. The VitD/VitD offspring displayed metabolic changes and reduction in the cross-sectional area of the penis, corpus cavernosum, tunica albuginea, and increased area of the corpus spongiosum. The connective tissue, smooth muscle, and cell proliferation percentages were greater in the corpus cavernosum and corpus spongiosum in the VitD/VitD offspring. The percentages of sinusoidal spaces and elastic fibers in the corpus cavernosum decreased. The elastic fibers in the tunica albuginea of the corpus spongiosum in the VitD/VitD offspring were reduced. Vitamin D restriction during perinatal and postnatal periods induced metabolic and structural changes and represented important risk factors for erectile dysfunction in the penis of the adult offspring. These findings suggest that vitamin D is an important micronutrient in maintaining the cytoarchitecture of the penis.

When can immunotherapy for insect sting allergy be stopped?

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Müller, Ulrich R; Ring, Johannes

2015-01-01

Stings by Hymenoptera (honey bees, vespids, ants) can cause systemic allergic reactions (SARs). Venom immunotherapy (VIT) is highly effective and reduces an allergic patient's risk of a recurrent SAR to less than 5-20%. The risk of a recurrent SAR to a re-sting decreases the longer VIT is continued. The recommended duration of VIT is at least 3 to 5 years. Risk factors for recurrent SARs to a sting after stopping VIT have been identified and discussed: Recommendations concerning stopping VIT: For patients without any of the identified risk factors, VIT should be continued for 5 rather than 3 years. In patients with definite risk factors, a longer duration of VIT has to be discussed before stopping it. In mast cell disorders, VIT for life is recommended. Because of the residual risk of SARs after VIT, all patients are advised to carry an epinephrine autoinjector indefinitely and to continue to take measures to avoid Hymenoptera stings. Copyright © 2015 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

Evaluation of the satisfaction level of patients attended by a pharmaceutical care program in a private communitarian pharmacy in Vitória (ES, Brazil

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Tadeu Uggere de Andrade

2009-06-01

Full Text Available The present study was designed to evaluate the satisfaction of users of a Pharmaceutical Care (PC service in a private communitarian pharmacy in Vitória (ES, Brazil. In this transversal observational study, patient interviews were performed by an experimenter that had no relationship with the establishments evaluated. Data were collected using a structured questionnaire. The questionnaire used a five point Likert scale, in which smaller numbers represented lower levels of satisfaction. For comparison, user satisfaction was also evaluated for two pharmaceutical establishments that do not have standardized PC services. In these cases, age-matched patients were selected randomly. A higher level of satisfaction was reported by users of the PC service, with values between three and five. The higher averages for the establishment with PC service were the result of greater perceived pharmacist interest in the patient's health. As the same results were not obtained by the services without PC, it was concluded that this practice is very important to the satisfaction level of users of pharmacy services.O presente estudo teve por objetivo avaliar a satisfação de usuários de um serviço de atenção farmacêutica, implantado em uma farmácia comunitária privada no município de Vitória - ES. Trata-se de um estudo observacional de corte transversal, cuja coleta dos dados se deu por meio da aplicação de um questionário de satisfação. O instrumento foi aplicado por indivíduo treinado e sem vínculo com os estabelecimentos avaliados. Foi utilizada uma escala de intensidade de cinco pontos do tipo Likert, cujo menor número representa a opção "ruim" e o maior à opção "excelente". Também foi avaliado, para efeito de comparação, o nível de satisfação de usuários de dois outros estabelecimentos farmacêuticos que não possuíam esta prática implantada. Neste caso, os pacientes foram selecionados de forma aleatória, porém pareados pela

TURISMO CEMITERIAL: proposta de roteirização a partir das potencialidades turísticas impressas no cemitério civil de Santa Vitória do Palmar, RS, Brasil

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Cláudia Brandão Schwab

2017-09-01

Full Text Available Este trabalho apresenta projeto elaborado durante estágio curricular obrigatório, no qual se analisaram as possibilidades de transformação do Cemitério Civil de Santa Vitória do Palmar em um atrativo turístico, a partir da realização de trilhas interpretativas em um processo de roteirização, com vistas à preservação de seu patrimônio. A metodologia utilizada foi análise SWOT partindo da inventariação turística. As incursões exploratórias foram desenvolvidas por meio de pesquisa bibliográfica e em periódicos. A pesquisa de campo realizada fez levantamento da infraestrutura, acessibilidade, segurança do espaço e elementos tumulares visando à elaboração do plano final de roteirização.

Vitamin D vitamers affect vitamin D status differently in young healthy males

DEFF Research Database (Denmark)

Jakobsen, Jette; Wreford Andersen, Elisabeth Anne; Christensen, Tue

2018-01-01

-hydroxyvitamin D in serum (vitamin D status). To test our hypothesis, we performed a randomized, crossover study. Twelve young males consumed 10 µg/day vitD3 during a four-week run-in period, followed by 3 × 6 weeks of 10 µg/day vitD3, 10 µg/day 25OH-D3, and 10 µg/day vitD2. The content of vitD3, vitD2, 25OH-D3...

Effects of Ascorbic Acid on the Amplitude of Ventral Tegmental Area Field Action Potential in Morphine-Exposed Rats (An Electrophysiology Study

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K Saadipour

2010-07-01

Full Text Available Introduction & Objective: Evidences have indicated that the Ventral Tegmental Area (VTA is the major source of dopamine (DA neurons projecting to cortical and limbic regions involved in cognitive and motivational aspects of addiction. Also, studies have indicated that the Ascorbic acid (vitamin C can reduce the dependency symptoms of opioids such as morphine via effect of activity on dopaminergic neuron in VTA. For this reason, the aim of this study was to assess the effects of ascorbic acid on the amplitude of Ventral Tegmental Area field action potential in morphine-exposed rats. Materials & Methods: Forty male Wistar’s rats were used in this experimental study conducted at Yasuj University of Medical Sciences in 2010. Animals were randomly divided into four groups after electrode implantation and recovery period: 1. No- Vit C and No-Addicted group (nVitC.nA 2. Vit C and No-Addicted group (VitC.nA 3. No- Vit C and Addicted group (nVitCA 4.Vit C and Addicted (VitC.A, The Vit C groups received 500 mg/kg of Vit C during 20 days. For addicted groups morphine was administrated once daily for 20 days. In the 20th day, the field potential recording was accomplished. Two-way ANOVA was used for data analysis followed by the Tukey test for post hoc analysis. Results were considered significant at P < 0.05. Results: This study shows the exposure to morphine declined the power of Delta and Beta bands (p<0.05 and Vit C solely enhance power of Theta and Beta (p<0.05, p<0.001 in VTA nuclei. Furthermore, Vit C could alter power of some bands which were affected by morphine. Therefore it seems that Vit C has an increasing effects on them (p<0.05. Conclusion: Although the effect of Vit C on power of the VTA bands is not well known, but it is supposed that this phenomenon can be related to alteration in activity of dopaminergic neuron in the brain.

Decreased Serum Vitamin D Levels in Children with Asthma are Associated with Increased Corticosteroid Usage

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Searing, Daniel A.; Zhang, Yong; Murphy, James R.; Hauk, Pia J.; Goleva, Elena; Leung, Donald Y. M.

2010-01-01

Background There is little knowledge about clinical variables associated with vitamin D (vitD) insufficiency in asthmatic children. Objective To investigate disease variables associated with vitD insufficiency in childhood asthma and interaction of vitD with corticosteroid-mediated anti-inflammatory responses. Methods We analyzed 25-hydroxyvitamin D serum levels in 100 asthmatic children to investigate relationships between 25-hydroxyvitamin D levels and patient characteristics. We determined vitD effects on dexamethasone (DEX) induction of mitogen-activated protein kinase phosphatase-1 (MKP-1) and IL-10 in peripheral blood mononuclear cells (PBMC). Results The median 25-hydroxyvitamin D serum level was 31 ng/mL. 47% of subjects had vitD levels in the insufficient range (<30 ng/mL), while 17% were vitD deficient (<20 ng/mL). Log10 IgE (p=0.01, ρ=−0.25) and the number of positive aeroallergen skin prick tests (p=0.02, ρ=−0.23) showed a significant inverse correlation with vitD, whereas FEV1% predicted (p =0.004, ρ=0.34) and FEV1/FVC ratio (p=0.01, ρ=0.30) showed a significant positive correlation with vitD. The use of inhaled steroids (p=0.0475), oral steroids (p=0.02), and total steroid dose (p=0.001), all showed significant inverse correlations with vitD. The amount of MKP-1 and IL-10 mRNA induced by vitD plus DEX was significantly greater than that induced by DEX alone (p<0.01). In an experimental model of steroid resistance where DEX alone did not inhibit T cell proliferation, addition of vitD to DEX resulted in significant dose dependent suppression of cell proliferation. Conclusions Corticosteroid use and worsening airflow limitation is associated with lower vitD serum levels in asthmatics. VitD enhances glucocorticoid action in asthmatic PBMC and enhances the immunosuppressive function of DEX in vitro. Clinical Implications Our study suggests that vitD supplementation may potentiate anti-inflammatory function of corticosteroids in asthmatics and

Sistema de espaços livres e forma urbana na Ilha de Vitória, Espírito Santo, Brasil

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Eneida Maria Souza Mendonça

2014-06-01

Full Text Available O debate apresentado neste artigo trata das relações entre o sistema de espaços livres e a forma urbana de Vitória, capital do Estado do Espírito Santo, no Sudeste brasileiro, no território específico referente à ilha. A decisão quanto à área de estudo tem como fator fundamental o fato desta ter abrigado a origem da ocupação do lugar, em meados do século XVI, por colonizadores portugueses, e, portanto, conter, desde então, sucessivas e diversificadas formas de ocupação. A metodologia adotada compreende, principalmente, o levantamento dos espaços livres públicos e privados por imagens de satélite, e, quando necessário, visitas de campo, e o estudo relacionado à evolução urbana. A pesquisa possibilitou a identificação de morfologias distintas, caracterizadas em função do processo histórico de ocupação urbana da área e sua relação com o sítio físico. Cabe ainda observar que o território insular, constituído originalmente por morros e estreita faixa de terra entre estes e o mar, identificado como porto seguro pelos colonizadores portugueses, só evoluiu para o atual centro metropolitano em função dos sucessivos aterros realizados ao longo do tempo.

Modelo geológico de substrato sedimentar marinho aplicado à atividade de expansão do canal de acesso e bacia de evolução do porto de tubarão em Vitória/ ES.

OpenAIRE

Carneiro, Maurício de Souza

2013-01-01

Programa de Pós-Graduação em Engenharia Geotécnica. Núcleo de Geotecnia, Escola de Minas, Universidade Federal de Ouro Preto. O Porto de Tubarão, localizado em Vitória, no Estado do Espírito Santo, administrado pela empresa de mineração Vale S/A, é o maior exportador de minério e pelotas de ferro do mundo. Localiza-se na ponta de Tubarão, na área continental do município, movimentando além de minério de ferro, grãos, fertilizantes, carvão e combustíveis. Para a segura movimentação dos navi...

Maternal vitamin C deficiency during pregnancy results in transient fetal and placental growth retardation in guinea pigs

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Schjoldager, Janne Gram; Paidi, Maya Devi; Lindblad, Maiken Marie

2015-01-01

PURPOSE: Recently, we reported that preferential maternal-fetal vitamin C (vitC) transport across the placenta is likely to be impaired by prolonged maternal vitC deficiency. Maintenance of a basal maternal vitC supply at the expense of the fetus may impair fetal development; however, the knowled......, the present data suggest that vitC plays a role in early fetal development. Low maternal vitC intake during pregnancy may compromise maternal weight gain, placental function and intrauterine development....

Attenuation of Red Blood Cell Storage Lesions with Vitamin C

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Kimberly Sanford

2017-07-01

Full Text Available Stored red blood cells (RBCs undergo oxidative stress that induces deleterious metabolic, structural, biochemical, and molecular changes collectively referred to as “storage lesions”. We hypothesized that vitamin C (VitC, reduced or oxidized would reduce red cell storage lesions, thus prolonging their storage duration. Whole-blood-derived, leuko-reduced, SAGM (saline-adenine-glucose-mannitol-preserved RBC concentrates were equally divided into four pediatric storage bags and the following additions made: (1 saline (saline; (2 0.3 mmol/L reduced VitC (Lo VitC; (3 3 mmol/L reduced VitC (Hi VitC; or (4 0.3 mmol/L oxidized VitC (dehydroascorbic acid, DHA as final concentrations. Biochemical and rheological parameters were serially assessed at baseline (prior to supplementation and Days 7, 21, 42, and 56 for RBC VitC concentration, pH, osmotic fragility by mechanical fragility index, and percent hemolysis, LDH release, glutathione depletion, RBC membrane integrity by scanning electron microscopy, and Western blot for β-spectrin. VitC exposure (reduced and oxidized significantly increased RBC antioxidant status with varying dynamics and produced trends in reduction in osmotic fragility and increases in membrane integrity. Conclusion: VitC partially protects RBC from oxidative changes during storage. Combining VitC with other antioxidants has the potential to improve long-term storage of RBC.

Three days rush venom immunotherapy in bee allergy: safe, inexpensive and instantaneously effective.

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Goldberg, Arnon; Yogev, Ayala; Confino-Cohen, Ronit

2011-01-01

Rush venom immunotherapy (VIT) is highly effective in vespid venom allergy, but comparable data regarding bee venom (BV) allergy are sparse. We evaluated its safety, efficacy and cost in BV-allergic patients. Conventional or rush VIT were offered to all patients with systemic reaction to insect sting. Rush VIT was also given to hyperreactive patients who failed to reach the maintenance dose with conventional VIT due to multiple systemic reactions. In BV-allergic patients, honeybee sting challenge was performed within 1 week after reaching the maintenance dose. 179 patients, some of them allergic to more than one venom, received 246 rush VIT courses. Bee VIT was administered to 132 patients (73.7%); 173 patients (96.6%) reached the maintenance dose. The incidence of systemic reactions was 29.6%. They were more common in VIT with BV than with vespid venoms (31.1 and 16.3%, respectively, p = 0.01). After excluding the hyperreactive subgroup (n = 20), this difference was not significant (23.7 and 16%, respectively, p = 0.19). Despite the high incidence of systemic reactions (15 of 20, 75%) among hyperreactive patients, 17 patients (85%) achieved the maintenance dose. Sting challenges resulted in systemic reaction in 4 of 8 (50%) hyperreactive patients and in 2 of 47 (4.3%) ordinary patients. The cost of rush VIT was 41% of that of conventional VIT. Rush VIT with BV is safe, instantaneously effective, less expensive and enables most patients with previous failures of conventional VIT to reach the maintenance dose. Copyright © 2011 S. Karger AG, Basel.

Association of Vitamin D Deficiency and Thyroid Function in Postmenopausal Women

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Mitra Niafar

2016-12-01

Full Text Available Pupose: Although there are reports of vitamin D (VitD insufficiency in immune-mediated hypothyroidism, an association between VitD and thyroid-stimulating hormone (TSH levels has yet to be shown. We aim to examine VitD and TSH levels among postmenopausal women, as both conditions are more prevalent in elderly women. Methods: The clinic records of postmenopausal women during their routine maintenance visits were reviewed. All patients were examined for the symptoms related to thyroid function and osteoporosis. Participants were divided into three subgroups according to their TSH levels (below 4.0 mIU/L. Patient characteristics and VitD levels were compared between these subgroups. Multivariate linear regression model was constructed using serum VitD and serum TSH as the dependent variables to identify factors independently associated with these laboratory values. Results: Two-hundred and nighty nine postmenopausal women were included. Average age was 62.2±7.5 years old. VitD was insufficient (10-30 ng/mL in 12.0% and deficient (<10 ng/mL in 60.9% of the participants. In 11.3%, TSH was low and in 7.6% of women, TSH was high, while the remaining 80.1%, had normal TSH levels. Subjects with low TSH had significantly higher VitD concentrations (34.2±29.1 ng/mL compared to the other two groups (P-value: 0.039. In multivariate regression analysis, TSH was not a contributing factor, as age was the only significant predictor of VitD levels. Meanwhile, no predictor (including age and VitD was identified for TSH levels in linear regression analysis. Conclusion: Age was the only independent predictor of serum VitD in this study population. Though suppressed TSH was associated with higher VitD levels, the association was not linear between TSH and VitD in postmenopausal women.

In vivo vitamin C deficiency in guinea pigs increases ascorbate transporters in liver but not kidney and brain.

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Søgaard, Ditte; Lindblad, Maiken M; Paidi, Maya D; Hasselholt, Stine; Lykkesfeldt, Jens; Tveden-Nyborg, Pernille

2014-07-01

Moderate vitamin C (vitC) deficiency (plasma concentrations less than 23 μmol/L) affects as much as 10% of adults in the Western World and has been associated with an increased mortality in disease complexes such as cardiovascular disease and the metabolic syndrome. The distribution of vitC within the body is subjected to complex and nonlinear pharmacokinetics and largely depends on the sodium-dependent vitC-specific transporters, sodium-dependent vitamin C transporter 1 (SVCT1) and sodium-dependent vitamin C transporter 2 (SVCT2). Although currently not established, it is likely to expect that a state of deficiency may affect the expression of these transporters to preserve vitC concentrations in specific target tissues. We hypothesized that diet-induced states of vitC deficiency lead to alterations in the messenger RNA (mRNA) and/or protein expression of vitC transporters, thereby regulating vitC tissue distribution. Using guinea pigs as a validated model, this study investigated the effects of a diet-induced vitC deficiency (100 mg vitC/kg feed) or depletion (0 mg vitC/kg feed) on the expression of transporters SVCT1 and SVCT2 in selected tissues and the transport from plasma to cerebrospinal fluid (CSF). In deficient animals, SVCT1 was increased in the liver, whereas a decreased SVCT1 expression but increased SVCT2 mRNA in livers of depleted animals suggests a shift in transporter expression as response to the diet. In CSF, a constant plasma:CSF ratio shows unaltered vitC transport irrespective of dietary regime. The study adds novel information to the complex regulation maintaining vitC homeostasis in vivo during states of deficiency. Copyright © 2014 Elsevier Inc. All rights reserved.

Effect of combination vitamin E and single long-acting progesterone dose on enhancing pregnancy outcomes in the first two parities of young rabbit does.

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Salem, Anas A; Gomaa, Yasmin A

2014-11-10

Vitamin E (Vit. E) is needed for young rabbits to prevent reproductive abnormalities, abortion and poor survivability of kits. Also, exogenous progesterone (P4) is needed for rabbits to enhance early embryonic development because of inadequate corpus luteum (CL) development at this age. Hence, the aim of this study was to investigate the effect of injecting Vit. E and the combination Vit. E+P4 in young does on live body weight (LBW) gain, gestation length (GL), numbers of services/conception (NS), conception rate (CR), abortion rate (AR), litter size (LS), kit weight (KW), total litter weight (TLW), mortality rate (MR) and progesterone (P4) concentration. The group treated with Vit. E+P4 had a greater LBW gain and lesser AR at first and second pregnancy. Treatments did not have significant impact on GL and LS in the first two parities. Treatments resulted in a significantly lesser MR and greater TLW at the second parity. The Vit. E+P4 treatment resulted in a significantly lesser NS at the first parity, while Vit. E alone resulted in a significant reduction in NS at the second parity. Vit. E+P4 had a positive effect on CR at the first parity compared with controls. Vit. E alone increased CR at the second parity compared with that of the control group. The mean P4 concentration from mating to mid-pregnancy at first parity was significantly greater in the Vit. E+P4 than Vit. E and control groups. In conclusion, treatment with Vit. E+P4 at the first parity may be economically applied on rabbit farms because this treatment resulted in a greater maintenance of the first pregnancy and improved reproductive performance at the second parity as compared with results from the Vit. E treated and control groups. Copyright © 2014 Elsevier B.V. All rights reserved.

Maternal vitamin D deficiency increases the risk of adverse neonatal outcomes in the Chinese population: A prospective cohort study.

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Yuanliu Wang

Full Text Available Although vitamin D (vitD deficiency is a common problem in pregnant women, in China, few studies have focused on the relationship between maternal vitD deficiency throughout the three trimesters and subsequent neonatal outcomes in China.Between 2015 and 2016, maternal serum and neonate cord blood samples were collected from 1978 mother-neonate pairs from Liuzhou city.The mean concentrations of 25-hydroxy vitD (25(OHD were 16.17±6.27 and 15.23±5.43 ng/ml in the mother and neonate groups, respectively, and the prevalence values of vitD deficiency in the two groups were 78.18% and 83.27%, respectively. Logistic regression showed that maternal vitD deficiency independently increased the risk of gestational diabetes mellitus (GDM (adjust OR, aOR 1.08; P = 0.026. A relatively lower risk of vitD deficiency was observed in the third trimester than in the first and second trimester (aOR 0.80; P = 0.004. VitD-calcium cosupplementation during pregnancy improves the vitD deficiency in both the maternal and neonatal groups (aOR 0.56, 0.66; P<0.001 and 0.021, respectively. Maternal vitD deficiency significantly increased the risk of neonatal low birth weight (LBW (aOR 2.83; P = 0.005 and small-for-gestational-age (SGA (aOR 1.17; P = 0.015. There was a positive correlation between maternal and neonatal vitD deficiency (r = 0.879, P<0.001. VitD supplementation during pregnancy significantly reduced the risk of giving birth to LBW infants (OR = 0.47, 95%CI = 0.33-0.68, P<0.001.Further research focusing on the consumption of vitD with calcium during pregnancy and the consequential clinical outcomes in Chinese pregnant women is warranted.

Vitamin D in schizophrenia: a clinical review.

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Chiang, Mathew; Natarajan, Radhika; Fan, Xiaoduo

2016-02-01

Vitamin D (vitD) is known for its essential role in calcium homeostasis and bone health. VitD is made endogenously in the skin from UVB radiation from sunlight. VitD is now considered as a potent neurosteroid hormone, critical to brain development and normal brain function, and is known for its anti-inflammatory property affecting various aspects of human health. VitD ligand-receptor, a receptor that mediates much of vitD's biological actions, has been found throughout the body including the central nervous system. VitD deficiency is common in patients with severe mental illness such as schizophrenia. Schizophrenia is a debilitating chronic mental illness characterised by positive symptoms, such as hallucinations and delusions, and negative symptoms including flat affect and lack of motivation. Several environmental risk factors for schizophrenia, such as season of birth, latitude and migration, have been linked to vitD deficiency. Recent studies have suggested a potential role of vitD in the development of schizophrenia. For example, neonatal vitD status is associated with the risk of developing schizophrenia in later life obesity, insulin resistance, diabetes, hyperlipidaemia and cardiovascular disease, which are commonly seen in patients with schizophrenia. It has been well established that vitD deficiency is related to these metabolic problems. The biological mechanism is most likely related to vitD's action on the regulation of inflammatory and immunological processes, consequently affecting the manifestation of clinical symptoms and treatment response of schizophrenia. Potential benefits of vitD supplementation to improve schizophrenia symptoms as well as physical health in patients with schizophrenia should be further explored in future studies. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

Anaphylactic Reactions After Discontinuation of Hymenoptera Venom Immunotherapy: A Clonal Mast Cell Disorder Should Be Suspected.

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Bonadonna, Patrizia; Zanotti, Roberta; Pagani, Mauro; Bonifacio, Massimiliano; Scaffidi, Luigi; Olivieri, Elisa; Franchini, Maurizio; Reccardini, Federico; Costantino, Maria Teresa; Roncallo, Chiara; Mauro, Marina; Boni, Elisa; Rizzini, Fabio Lodi; Bilò, Maria Beatrice; Marcarelli, Anna Rosaria; Passalacqua, Giovanni

2017-12-16

Up to 75% of patients with severe anaphylactic reactions after Hymenoptera sting are at risk of further severe reactions if re-stung. Venom immunotherapy (VIT) is highly effective in protecting individuals with ascertained Hymenoptera venom allergy (HVA) and previous severe reactions. After a 3- to 5-year VIT course, most patients remain protected after VIT discontinuation. Otherwise, a lifelong treatment should be considered in high-risk patients (eg, in mastocytosis). Several case reports evidenced that patients with mastocytosis and HVA, although protected during VIT, can re-experience severe and sometimes fatal reactions after VIT discontinuation. To evaluate whether patients who lost protection after VIT discontinuation may suffer from clonal mast cell disorders. The survey describes the characteristics of patients who received a full course of VIT for previous severe reactions and who experienced another severe reaction at re-sting after VIT discontinuation. Those with a Red Española de Mastocitosis score of 2 or more or a serum basal tryptase level of more than 25 ng/mL underwent a hematological workup (bone marrow biopsy, KIT mutation, expression of aberrant CD25) and/or skin biopsy. Nineteen patients (mean age, 56.3 years; 89.5% males) were evaluated. All of them had received at least 4 years of VIT and were protected. After VIT discontinuation they were re-stung and developed, in all but 1 case, severe anaphylactic reactions (12 with loss of consciousness, in the absence of urticaria/angioedema). Eighteen patients (94.7%) had a clonal mast cell disorder, 8 of them with normal tryptase. Looking at this selected population, we suggest that mastocytosis should be considered in patients developing severe reactions at re-sting after VIT discontinuation and, as a speculation, patients with mastocytosis and HVA should be VIT-treated lifelong. Copyright © 2017. Published by Elsevier Inc.

[Effect of vitamin C on the condition of NO-synthase system in experimental stomach ulcer].

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Zhuroms'kyĭ, V S; Skliarov, O Ia

2011-01-01

We investigated the effect of Vitamin C (Vit C) on the changes of activity of the enzymes of NO-synthase system, nitric oxide content, lipoperoxidation processes, activity of SOD and catalase in gastric mucosa (GM), and concentrations of L-arginine, Vit C and Vit E in the blood of rats under conditions of experimental ulcer of the stomach caused by adrenaline injection. Vit C displayed a pronounced antioxidant action, reduced the degree of destructive affections, diminished the activity of iNOS and lipoperoxidation processes, decreased the NO content and SOD activity. Furthermore, the concentration of L-arginine and Vit C in the blood was increased. Combined action of Vit C with L-arginine reduced the degree of GM lesions, activity of eNOS and the content of NO in GM whereas the concentration of L-arginine in blood was increased. Under conditions of Vit C action and iNOS and COX-2 blockage, the activity of NO-synthases and lipoperoxidation processes were slightly decreased, indicating on dominant action of Vit C.

High-fat feeding increases hepatic vitamin C synthesis and its circulatory mobilization in mice

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Christensen, Britt Tranberg; Hansen, Axel Jacob Kornerup; Lykkesfeldt, Jens

2014-01-01

, glucose and vitC concentrations. Hepatic vitC concentration and gulonolactone oxidase (GLO) capacity, as a measure of vitC de novo biosynthesis, were analyzed in liver homogenates. RESULTS: HF diet significantly increased plasma concentrations of vitC compared with a control diet low in fat (P ... to modulate their vitC homeostasis during high-fat (HF) feeding. METHODS: Twenty-five male 5-week-old C57BL/6 mice were fed high- or low-fat diets for 14 weeks. An oral glucose tolerance test (OGTT) was performed after 12 weeks of intervention. Terminal fasting plasma samples were analyzed for insulin.......05). Hepatic de novo biosynthesis of vitC was upregulated (P glucose and insulin concentrations...

Decreased serum vitamin D levels in children with asthma are associated with increased corticosteroid use.

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Searing, Daniel A; Zhang, Yong; Murphy, James R; Hauk, Pia J; Goleva, Elena; Leung, Donald Y M

2010-05-01

There is little knowledge about clinical variables associated with vitamin D (VitD) insufficiency in asthmatic children. We sought to investigate disease variables associated with VitD insufficiency in patients with childhood asthma and interaction of VitD with corticosteroid-mediated anti-inflammatory responses. We analyzed 25-hydroxyvitamin D serum levels in 100 asthmatic children to investigate relationships between 25-hydroxyvitamin D levels and patients' characteristics. We determined VitD's effects on dexamethasone (DEX) induction of mitogen-activated protein kinase phosphatase 1 and IL-10 in PBMCs. The median 25-hydroxyvitamin D serum level was 31 ng/mL. Forty-seven percent of subjects had VitD levels in the insufficient range (<30 ng/mL), whereas 17% were VitD deficient (<20 ng/mL). Log(10) IgE (P = .01, rho = -0.25) and the number of positive aeroallergen skin prick test responses (P = .02, rho = -0.23) showed a significant inverse correlation with VitD levels, whereas FEV(1) percent predicted (P = .004, rho = 0.34) and FEV(1)/forced vital capacity ratio (P = .01, rho = 0.30) showed a significant positive correlation with VitD levels. The use of inhaled steroids (P = .0475), use of oral steroids (P = .02), and total steroid dose (P = .001) all showed significant inverse correlations with VitD levels. The amount of mitogen-activated protein kinase phosphatase 1 and IL10 mRNA induced by VitD plus DEX was significantly greater than that induced by DEX alone (P < .01). In an experimental model of steroid resistance in which DEX alone did not inhibit T-cell proliferation, addition of VitD to DEX resulted in significant dose-dependent suppression of cell proliferation. Corticosteroid use and worsening airflow limitation are associated with lower VitD serum levels in asthmatic patients. VitD enhances glucocorticoid action in PBMCs from asthmatic patients and enhances the immunosuppressive function of DEX in vitro. Copyright 2010 American Academy of Allergy

Safety and efficacy of venom immunotherapy: a real life study.

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Kołaczek, Agnieszka; Skorupa, Dawid; Antczak-Marczak, Monika; Kuna, Piotr; Kupczyk, Maciej

2017-04-01

Venom immunotherapy (VIT) is recommended as the first-line treatment for patients allergic to Hymenoptera venom. To analyze the safety and efficacy of VIT in a real life setting. One hundred and eighty patients undergoing VIT were studied to evaluate the safety, efficacy, incidence and nature of symptoms after field stings and adverse reactions to VIT. Significantly more patients were allergic to wasp than bee venom (146 vs. 34, p bees, and were not associated with angiotensin convertase inhibitors (ACEi) or β-adrenergic antagonists use. Systemic reactions were observed in 4 individuals on wasp VIT (2.7%) and in 6 patients allergic to bees (17.65%). The VIT was efficacious as most patients reported no reactions (50%) or reported only mild local reactions (43.75%) to field stings. The decrease in sIgE at completion of VIT correlated with the dose of vaccine received ( r = 0.53, p = 0.004). Beekeeping (RR = 29.54, p venom allergy. Venom immunotherapy is highly efficacious and safe as most of the adverse events during the induction and maintenance phase are mild and local. Side effects of VIT are more common in subjects on bee VIT. Beekeeping and female sex are associated with a higher risk of allergy to Hymenoptera venom.

NO-dependent proliferation and migration induced by Vitamin D in HUVEC.

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Pittarella, Pamela; Squarzanti, Diletta F; Molinari, Claudio; Invernizzi, Marco; Uberti, Francesca; Renò, Filippo

2015-05-01

Recently, Vitamin D (Vit. D) has gained importance in cellular functions of a wide range of extraskeletal organs and target tissues, other than bone. In particular, Vit. D has displayed important beneficial effects in the cardiovascular system. Although little is known about the mechanism by which this response is exerted, a Vit. D-induced eNOS-dependent nitric oxide (NO) production in endothelial cells (EC) has been reported. The aim of this study was to evaluate whether Vit. D administration could affect human EC proliferation and/or migration through NO production. For this purpose, HUVEC (human umbilical vein endothelial cells) were used to evaluate Vit. D effects on cell proliferation and migration in a 3D matrix. Experiments were also performed in the presence of the specific VDR ligand ZK159222 and eNOS inhibitor L-NAME. This study demonstrated that Vit. D can promote both HUVEC proliferation and migration in a 3D matrix. These effects were NO dependent, since HUVEC proliferation and migration were abrogated along with Vit. D induced MMP-2 expression by inhibiting eNOS activity by L-NAME. These findings support the role of Vit. D in the angiogenic process, suggesting new applications for Vit. D in tissue repair and wound healing. Copyright © 2015 Elsevier Ltd. All rights reserved.

Distribution of vitamin C is tissue specific with early saturation of the brain and adrenal glands following differential oral dose regimens in guinea pigs

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Andersen, Stine Hasselholt; Tveden-Nyborg, Pernille; Lykkesfeldt, Jens

2015-01-01

Vitamin C (VitC) deficiency is surprisingly common in humans even in developed parts of the world. The micronutrient has several established functions in the brain; however, the consequences of its deficiency are not well characterised. To elucidate the effects of VitC deficiency on the brain......, increased knowledge about the distribution of VitC to the brain and within different brain regions after varying dietary concentrations is needed. In the present study, guinea pigs (like humans lacking the ability to synthesise VitC) were randomly divided into six groups (n 10) that received different...... concentrations of VitC ranging from 100 to 1500 mg/kg feed for 8 weeks, after which VitC concentrations in biological fluids and tissues were measured using HPLC. The distribution of VitC was found to be dynamic and dependent on dietary availability. Brain saturation was region specific, occurred at low dietary...

Poor Vitamin C Status Late in Pregnancy Is Associated with Increased Risk of Complications in Type 1 Diabetic Women: A Cross-Sectional Study

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Juhl, Bente; Lauszus, Finn Friis; Lykkesfeldt, Jens

2017-01-01

Vitamin C (vitC) is essential for normal pregnancy and fetal development and poor vitC status has been related to complications of pregnancy. We have previously shown lower vitC status in diabetic women throughout pregnancy compared to that of non-diabetic controls. Here, we evaluate...... the relationship between vitC status late in diabetic pregnancy in relation to fetal outcome, complications of pregnancy, diabetic characteristics, and glycemic control based on data of 47 women from the same cohort. We found a significant relationship between the maternal vitC level > or ≤ the 50% percentile...... of 26.6 μmol/L, respectively, and the umbilical cord blood vitC level (mean (SD)): 101.0 μmol/L (16.6) versus 78.5 μmol/L (27.8), p = 0.02; n = 12/16), while no relation to birth weight or Apgar score was observed. Diabetic women with complications of pregnancy had significantly lower vitC levels...

A inclusão na educação infantil de Vitória: contribuições da educação física

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Alexandre Freitas Marchiori

2015-10-01

Full Text Available http://dx.doi.org/10.5007/1980-4512.2015n31p292 O artigo objetiva compreender as possibilidades de uma prática compartilhada e desenvolver proposições que contribuíssem com os processos de inclusão na Educação Infantil. Os sujeitos são professores que atuam no Centro Municipal de Educação Infantil (CMEI de Vitória e duas crianças com necessidades educativas especiais (NEE. O referencial teórico-metodológico fundamenta-se na perspectiva histórico cultural (VYGOTSKY;, 2007; WALLON;, 2010. A perspectiva de professor reflexivo (NÓVOA, 2001 e as concepções metodológicas dos estudos nos/dos/com os cotidianos orienta a produção desse artigo. As fontes foram os registros fotográficos e filmagens das práticas pedagógicas em 2014 e relatórios avaliativos de dois alunos com NEE. A oferta da educação física no currículo da educação infantil amplia as possibilidades do trabalho colaborativo e potencializam as práticas da educação inclusiva.

Effect of vitamin B1 and mixtures of B1 with other vitamins on cytostatic efficiency of sanazole under irradiation. A study in vitro

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Heinrich, Edith; Getoff, Nikola

2003-01-01

Experiments in vitro, using bacteria Escherichia coli (AB 1157) as a biological model, showed that the cytostatic efficiency of sanazole (AK-2123, a nitrotriazole-type radiosensitizer) in radiation treatment can be strongly influenced by the presence of various vitamins. In airfree media the sanazole action is increased by a factor of 2.5 in the presence of vitamin (vit.) B1, vit. C E-acetate and β-carotene, whereas vit. B1 used individually possesses a 2.7-times higher cytostatic activity than sanazole itself. In media containing air the highest increase of sanazole action is observed in the presence of vit. B1 and C, whereas the individual use of vit. B1 shows a radiation protection effect. In media saturated with N 2 O the addition of the vit. B1 and C causes a 1.8-times larger sanazole activity, but the application of vit. B1 alone brings about a very high radiation protection. >From studies of vit. B1-radiolysis it can be concluded that OH radicals are the major primary transients leading to substrate degradation. The results are of interest for the radiation therapy of cancer

Effect of vitamin B1 and mixtures of B1 with other vitamins on cytostatic efficiency of sanazole under irradiation. A study in vitro

Science.gov (United States)

Heinrich, Edith; Getoff, Nikola

2003-06-01

Experiments in vitro, using bacteria Escherichia coli (AB 1157) as a biological model, showed that the cytostatic efficiency of sanazole (AK-2123, a nitrotriazole-type radiosensitizer) in radiation treatment can be strongly influenced by the presence of various vitamins. In airfree media the sanazole action is increased by a factor of 2.5 in the presence of vitamin (vit.) B1, vit. C E-acetate and β-carotene, whereas vit. B1 used individually possesses a 2.7-times higher cytostatic activity than sanazole itself. In media containing air the highest increase of sanazole action is observed in the presence of vit. B1 and C, whereas the individual use of vit. B1 shows a radiation protection effect. In media saturated with N 2O the addition of the vit. B1 and C causes a 1.8-times larger sanazole activity, but the application of vit. B1 alone brings about a very high radiation protection. From studies of vit. B1-radiolysis it can be concluded that OH radicals are the major primary transients leading to substrate degradation. The results are of interest for the radiation therapy of cancer.

Effect of vitamin B1 and mixtures of B1 with other vitamins on cytostatic efficiency of sanazole under irradiation. A study in vitro

Energy Technology Data Exchange (ETDEWEB)

Heinrich, Edith; Getoff, Nikola E-mail: nikola.getoff@univie.ac.at

2003-06-01

Experiments in vitro, using bacteria Escherichia coli (AB 1157) as a biological model, showed that the cytostatic efficiency of sanazole (AK-2123, a nitrotriazole-type radiosensitizer) in radiation treatment can be strongly influenced by the presence of various vitamins. In airfree media the sanazole action is increased by a factor of 2.5 in the presence of vitamin (vit.) B1, vit. C E-acetate and {beta}-carotene, whereas vit. B1 used individually possesses a 2.7-times higher cytostatic activity than sanazole itself. In media containing air the highest increase of sanazole action is observed in the presence of vit. B1 and C, whereas the individual use of vit. B1 shows a radiation protection effect. In media saturated with N{sub 2}O the addition of the vit. B1 and C causes a 1.8-times larger sanazole activity, but the application of vit. B1 alone brings about a very high radiation protection. >From studies of vit. B1-radiolysis it can be concluded that OH radicals are the major primary transients leading to substrate degradation. The results are of interest for the radiation therapy of cancer.

Vitamin C: A Novel Regulator of Neutrophil Extracellular Trap Formation

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Ramesh Natarajan

2013-08-01

Full Text Available Introduction: Neutrophil extracellular trap (NET formation was recently identified as a novel mechanism to kill pathogens. However, excessive NET formation in sepsis can injure host tissues. We have recently shown that parenteral vitamin C (VitC is protective in sepsis. Whether VitC alters NETosis is unknown. Methods: We used Gulo−/− mice as they lack the ability to synthesize VitC. Sepsis was induced by intraperitoneal infusion of a fecal stem solution (abdominal peritonitis, FIP. Some VitC deficient Gulo−/− mice received an infusion of ascorbic acid (AscA, 200 mg/kg 30 min after induction of FIP. NETosis was assessed histologically and by quantification for circulating free DNA (cf-DNA in serum. Autophagy, histone citrullination, endoplasmic reticulum (ER stress, NFκB activation and apoptosis were investigated in peritoneal PMNs. Results: Sepsis produced significant NETs in the lungs of VitC deficient Gulo−/− mice and increased circulating cf-DNA. This was attenuated in the VitC sufficient Gulo−/− mice and in VitC deficient Gulo−/− mice infused with AscA. Polymorphonuclear neutrophils (PMNs from VitC deficient Gulo−/− mice demonstrated increased activation of ER stress, autophagy, histone citrullination, and NFκB activation, while apoptosis was inhibited. VitC also significantly attenuated PMA induced NETosis in PMNs from healthy human volunteers.

Rush immunotherapy for wasp venom allergy seems safe and effective in patients with mastocytosis.

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Verburg, M; Oldhoff, J M; Klemans, R J B; Lahey-de Boer, A; de Bruin-Weller, M S; Röckmann, H; Sanders, C; Bruijnzeel-Koomen, C A F M; Pasmans, S G M A; Knulst, A C

2015-11-01

Patients with mastocytosis and wasp venom allergy (WA) may benefit from venom immunotherapy (VIT). However, fatal insect sting reactions have been described in mastocytosis patients despite previous immunotherapy. We investigated the safety and efficacy of (rush) VIT in patients with mastocytosis and WA. To investigate the safety and efficacy of (rush) VIT in patients with mastocytosis and WA. We describe nine patients with cutaneous mastocytosis and WA who received VIT. Cutaneous mastocytosis was confirmed by histopathology and systemic mastocytosis was diagnosed according to World Health Organization criteria. VIT was given according to a rush protocol. Given the difference in safety and efficacy of VIT in patients with WA and honeybee venom allergy, we reviewed the literature for VIT with the focus on WA patients with mastocytosis and addressed the difference between patients with cutaneous versus systemic mastocytosis. Nine patients had WA and mastocytosis, of whom six had cutaneous mastocytosis, two combined cutaneous and systemic mastocytosis and one systemic mastocytosis. All patients received rush IT with wasp venom. Most patients had only mild local side effects, with no systemic side effects during the course of VIT. One patient had a systemic reaction upon injection on one occasion, during the updosing phase, with dyspnoea and hypotension, but responded well to treatment. Immunotherapy was continued after temporary dose adjustment without problems. Two patients with a previous anaphylactic reaction were re-stung, without any systemic effects. VIT is safe in cutaneous mastocytosis patients with WA, while caution has to be made in case of systemic mastocytosis. VIT was effective in the patients who were re-stung.

Cold-Induced Ascites in Broilers: Effects of Vitamin C and Coenzyme Q10

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MH Nemati

Full Text Available ABSTRACT We hypothesized that the supplementation of vitamin C (Vit. C and coenzyme Q10 (CoQ10 alone or in combination could reduce the negative effects of cold stress in broilers. Four hundred male chicks were exposed for 24 h to cold stress (15 ºC starting from 15d of age, while a positive control group (PC, 100 birds was kept under normal temperature condition. The experimental groups under cold stress (four treatments in 5 replicates of 20 birds were: negative control (NC, basal diet, Vit. C (basal diet + 300 mg/kg Vit. C, CoQ10 (basal diet + 40 mg/kg CoQ10 and Vit. C plus CoQ10 (basal diet + Vit. C+ CoQ10at above mentioned doses. Vit. C or CoQ10 supplementation were restored (p<0.01 performance and lowered (p<0.01 ascites mortality. Blood hematocrit and hemoglobin concentration were decreased (p<0.01 to the level comparable to PC by Vit. C supplementation. Lower plasma concentrations of thyroxin (T4 and higher triiodothyronine (T3 were observed in NC birds (p<0.01 and were not affected by Vit. C or CoQ10. In conclusion, supplementation of Vit. C or CoQ10 in diet of broilers under cold stress conditions resulted improved performance parameters (body weight and feed conversion ratio and ascites related traits (low red blood cell count, hematocrit, T3, and heart weights and high T4. No additional benefit was observed by combination of Vit. C and CoQ10.

The association of vitamin D, cathelicidin, and vitamin D binding protein with acute asthma attacks in children.

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Arikoglu, Tugba; Kuyucu, Semanur; Karaismailoglu, Eda; Batmaz, Sehra Birgul; Balci, Senay

2015-01-01

Recent evidence about the various effects of vitamin D (vit D) on innate and adaptive immunity has led to a search for the role of vit D in asthma. It is postulated that a decrease in cathelicidin, a multifunctional host defense molecule, production due to low vit D status may predispose to infectious complications in children with asthma. The aim of this study was to determine the association of vit D, vit D-binding protein (VDBP) and cathelicidin with acute asthma attacks among children with allergic asthma. This prospective study included 35 patients with acute asthma attack and 32 children with controlled asthma, and all were matched by sampling season, sensitization to mites, and previous severity of asthma. A comprehensive questionnaire about risk factors, blood sampling for 25-hydroxyvitamin D vit D, VDBP, and cathelicidin levels; spirometric indices were used. Factors that influence serum vit D and cathelicidin levels and the development of asthma attacks were evaluated with multivariate analysis. The mean serum vit D levels of the attack group was significantly lower than that of the controlled asthma group (p asthma group than with the controlled subjects with asthma (p = 0.002). There was no difference between the acute and controlled asthma groups in terms of markers of allergy and serum VDBP levels. Risk factors that may influence vit D levels revealed that body mass index (BMI) (p = 0.038), duration of sun exposure (p asthma showed that low serum levels of vit D were significantly related to the risk of asthma attacks (p asthma attacks and BMI. Vit D deficiency showed a significant relationship to the development of asthma attacks independent of cathelicidin deficiency and other factors associated with the severity of chronic asthma.

Vitamin D Insufficiency and Cognitive Function Trajectories in Older Adults: The Rancho Bernardo Study.

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Laughlin, Gail A; Kritz-Silverstein, Donna; Bergstrom, Jaclyn; Reas, Emilie T; Jassal, Simerjot K; Barrett-Connor, Elizabeth; McEvoy, Linda K

2017-01-01

Evidence of a role for vitamin D (VitD) in cognitive aging is mixed and based primarily on extreme VitD deficiency. We evaluated the association of VitD insufficiency with cognitive function in older, community-dwelling adults living in a temperate climate with year-round sunshine. A population-based longitudinal study of 1,058 adults (median age 75; 62% women) who had cognitive function assessed and serum levels of 25-hydroxyvitaminD (25OHD) measured in 1997-99 and were followed for up to three additional cognitive function assessments over a 12-year period. Overall, 14% (n = 145) of participants had VitD insufficiency defined as 25OHD age, sex, education, and season, VitD insufficiency was associated with poorer baseline performance on the Mini-Mental Status Exam (MMSE) (p = 0.013), Trails Making Test B (Trails B) (p = 0.015), Category Fluency (p = 0.006), and Long Term Retrieval (p = 0.019); differences were equivalent to 5 years of age. For those with VitD insufficiency, the odds of mildly impaired performance at baseline were 38% higher for MMSE (p = 0.08), 78% higher for Trails B (p = 0.017), and 2-fold higher for Category Fluency and Long Term Retrieval (both p = 0.001). VitD insufficiency was not related to the rate of cognitive decline on any test or the risk of developing impaired performance during follow-up. In this population with little VitD deficiency, even moderately low VitD was associated with poorer performance on multiple domains of cognitive function. Low VitD did not predict 12-year cognitive decline. Clinical trials are essential to establish a causal link between VitD and cognitive well-being.

L-Ascorbate attenuates methamphetamine neurotoxicity through enhancing the induction of endogenous heme oxygenase-1

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Huang, Ya-Ni [Department of Nursing, Hsin Sheng College of Medical Care and Management, Taoyuan, Taiwan (China); Wang, Jiz-Yuh [Department of Neurology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan (China); Lee, Ching-Tien [Department of Nursing, Hsin Sheng College of Medical Care and Management, Taoyuan, Taiwan (China); Lin, Chih-Hung [Graduate Institute of Medical Sciences and Department of Physiology, College of Medicine, Taipei Medical University, Taipei, Taiwan (China); Lai, Chien-Cheng [Far Eastern Memorial Hospital, Department of Surgery, Taipei, Taiwan (China); Wang, Jia-Yi, E-mail: jywang2010@tmu.edu.tw [Graduate Institute of Medical Sciences and Department of Physiology, College of Medicine, Taipei Medical University, Taipei, Taiwan (China)

2012-12-01

Methamphetamine (METH) is a drug of abuse which causes neurotoxicity and increased risk of developing neurodegenerative diseases. We previously found that METH induces heme oxygenase (HO)-1 expression in neurons and glial cells, and this offers partial protection against METH toxicity. In this study, we investigated the effects of L-ascorbate (vitamin C, Vit. C) on METH toxicity and HO-1 expression in neuronal/glial cocultures. Cell viability and damage were evaluated by 3-(4,5-dimethylthianol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) reduction and lactate dehydrogenase (LDH) release, respectively. Neuronal and glial localization of HO-1 were identified by double immunofluorescence staining. Reactive oxygen species (ROS) production was measured using the fluorochrome 2′,7′-dichlorofluorescin diacetate. HO-1 mRNA and protein expression were examined by RT-qPCR and Western blotting, respectively. Results show that Vit. C induced HO-1 mRNA and protein expressions in time- and concentration-dependent manners. Inhibition of p38 mitogen-activated protein kinase (MAPK) but not extracellular signal-regulated kinase (ERK) significantly blocked induction of HO-1 by Vit. C. HO-1 mRNA and protein expressions were significantly elevated by a combination of Vit. C and METH, compared to either Vit. C or METH alone. Pretreatment with Vit. C enhanced METH-induced HO-1 expression and attenuated METH-induced ROS production and neurotoxicity. Pharmacological inhibition of HO activity abolished suppressive effects of Vit. C on METH-induced ROS production and attenuated neurotoxicity. We conclude that induction of HO-1 expression contributes to the attenuation of METH-induced ROS production and neurotoxicity by Vit. C. We suggest that HO-1 induction by Vit. C may serve as a strategy to alleviate METH neurotoxicity. -- Highlights: ► Besides the anti-oxidant effect, Vit. C also induces HO-1 expression in brain cells. ► Vit. C reduces METH neurotoxicity and ROS production by

L-Ascorbate attenuates methamphetamine neurotoxicity through enhancing the induction of endogenous heme oxygenase-1

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Huang, Ya-Ni; Wang, Jiz-Yuh; Lee, Ching-Tien; Lin, Chih-Hung; Lai, Chien-Cheng; Wang, Jia-Yi

2012-01-01

Methamphetamine (METH) is a drug of abuse which causes neurotoxicity and increased risk of developing neurodegenerative diseases. We previously found that METH induces heme oxygenase (HO)-1 expression in neurons and glial cells, and this offers partial protection against METH toxicity. In this study, we investigated the effects of L-ascorbate (vitamin C, Vit. C) on METH toxicity and HO-1 expression in neuronal/glial cocultures. Cell viability and damage were evaluated by 3-(4,5-dimethylthianol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) reduction and lactate dehydrogenase (LDH) release, respectively. Neuronal and glial localization of HO-1 were identified by double immunofluorescence staining. Reactive oxygen species (ROS) production was measured using the fluorochrome 2′,7′-dichlorofluorescin diacetate. HO-1 mRNA and protein expression were examined by RT-qPCR and Western blotting, respectively. Results show that Vit. C induced HO-1 mRNA and protein expressions in time- and concentration-dependent manners. Inhibition of p38 mitogen-activated protein kinase (MAPK) but not extracellular signal-regulated kinase (ERK) significantly blocked induction of HO-1 by Vit. C. HO-1 mRNA and protein expressions were significantly elevated by a combination of Vit. C and METH, compared to either Vit. C or METH alone. Pretreatment with Vit. C enhanced METH-induced HO-1 expression and attenuated METH-induced ROS production and neurotoxicity. Pharmacological inhibition of HO activity abolished suppressive effects of Vit. C on METH-induced ROS production and attenuated neurotoxicity. We conclude that induction of HO-1 expression contributes to the attenuation of METH-induced ROS production and neurotoxicity by Vit. C. We suggest that HO-1 induction by Vit. C may serve as a strategy to alleviate METH neurotoxicity. -- Highlights: ► Besides the anti-oxidant effect, Vit. C also induces HO-1 expression in brain cells. ► Vit. C reduces METH neurotoxicity and ROS production by

Vitamin D is associated with metabotropic but not neurotrophic effects of exercise in ovariectomized rats

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Parvin Babaei

2017-11-01

Full Text Available Abstract Purpose Here, we studied the beneficial effects of aerobic exercise on metabolic syndrome components, cognitive performance, brain derived neurotrophic factor (BDNF and irisin in ovariectomized rats with different serum vitamin D (Vit D status. Methods Eighty female wistar rats were divided into 2 groups of sham operated (sham, n = 8, and ovariectomized (OVX, n = 72. Then OVX were divided into 9 groups of receiving combination of exercise protocol with low dose of Vit D (OVX + EXE + LD, high dose of Vit D (OVX + EXE + HD, Vit D deficiency (OVX + EXE − D, and (OVX + EXE + Veh. Also non exercised groups of OVX receiving high dose of Vit D (OVX + HD, low dose of Vit D (OVX + LD, Vit D deficiency (OVX − D, and Veh (OVX + Veh were included. After 2 months of related interventions, spatial memory was assessed using Morris water maze (MWM, and then metabolic syndrome components were measured. Results High dose of Vit D supplementation showed significant reduction in weight (p = 0.001, lipid profiles (p = 0.001, visceral fat (p = 0.001 and waist circumference (p = 0.001 regardless of exercising or not, with no change in cognitiive function. Serum BDNF level was significantly higher in Vit D deficient group (p = 0.001, and was decreased in the OVX + HD. In contrary, irisin did not show any significant relationship with serum concentration of Vit D, while it was significantly elevated in the exercised groups compared with non-exercised counterparts. Conclusion Vit D insufficiency deteriorates metabolic syndrome components, and elevates serum BDNF as a compensatory metabotropic factor, and further supplementation significantly attenuates these components parallel with reduction in BDNF. In addition, aerobic exercise successfully induces various metabolic benefits, provided optimum serum level of Vit D.

Is Vitamin C Supplementation Beneficial on Plasma Levels of Vitamin C and Total Anitioxidants for Pediatric Thalassemic Patients Undergoing Hematopoietic Stem Cell Transplantation?

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Molouk Hadjibabaie

2015-10-01

Full Text Available Background: Thalassemic patients undergoing Hematopoietic Stem Cell Transplantation (HSCT are faced with cumulative high level of oxidative stress and depletion of critical antioxidants. Administration of antioxidants, is promising towards minimizing oxidative damage in both thalassemic and HSCT patients.Method: This was a prospective cross-sectional observational study. Patients as a part of institutional protocol were received Vitamin C (Vit C (all the patients received oral Vit C; 200 mg and 400 mg Vit C, if they were less or more than 20 kg respectively plus 10 mg/kg/day intravenous infusion of Vit C.We measured plasma Vit C and total antioxidant (TAs levels at four different time points; baseline, transplantation day (0, day +7 and day +14. We calculated mean and standard error for plasma levels of Vit C and TAs.Results: Fifthy patients enrolled in this study (mean age 7.97± 3.53. In all four time points, means of Vit C and TAs serum levels were under their reference values and their highest means were belong to baseline. Serum TAs and Vit C both depleted significantly from baseline to day 0 (P: 0.00 for both variables, then increased up to day +7 and it keeps rising till day +14 (P: 0.00 from day0 to day +7 and +14 for both variables. These changes were significant through the measurement time. There is also a significant correlation between baseline Vit C and baseline TAs (P: 0.11. This means the higher level of Vit C is correlated with higher level of TAs and vice versa.Conclusion: We did not observe any beneficial effects of administering Vit C in thalassemic patients undergoing HSCT in order to increase or prevent depletion of Vit C and TAs serum levels. This could be resolved by further investigations carrying out higher doses or longer duration and having a control group.

Vitamin D supplementation of initially vitamin D-deficient mice diminishes lung inflammation with limited effects on pulmonary epithelial integrity.

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Gorman, Shelley; Buckley, Alysia G; Ling, Kak-Ming; Berry, Luke J; Fear, Vanessa S; Stick, Stephen M; Larcombe, Alexander N; Kicic, Anthony; Hart, Prue H

2017-08-01

In disease settings, vitamin D may be important for maintaining optimal lung epithelial integrity and suppressing inflammation, but less is known of its effects prior to disease onset. Female BALB/c dams were fed a vitamin D 3 -supplemented (2280 IU/kg, VitD + ) or nonsupplemented (0 IU/kg, VitD - ) diet from 3 weeks of age, and mated at 8 weeks of age. Male offspring were fed the same diet as their mother. Some offspring initially fed the VitD - diet were switched to a VitD + diet from 8 weeks of age (VitD -/+ ). At 12 weeks of age, signs of low-level inflammation were observed in the bronchoalveolar lavage fluid (BALF) of VitD - mice (more macrophages and neutrophils), which were suppressed by subsequent supplementation with vitamin D 3 There was no difference in the level of expression of the tight junction proteins occludin or claudin-1 in lung epithelial cells of VitD + mice compared to VitD - mice; however, claudin-1 levels were reduced when initially vitamin D-deficient mice were fed the vitamin D 3 -containing diet (VitD -/+ ). Reduced total IgM levels were detected in BALF and serum of VitD -/+ mice compared to VitD + mice. Lung mRNA levels of the vitamin D receptor (VDR) were greatest in VitD -/+ mice. Total IgG levels in BALF were greater in mice fed the vitamin D 3 -containing diet, which may be explained by increased activation of B cells in airway-draining lymph nodes. These findings suggest that supplementation of initially vitamin D-deficient mice with vitamin D 3 suppresses signs of lung inflammation but has limited effects on the epithelial integrity of the lungs. © 2017 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

The effect of vitamin D on primary dysmenorrhea with vitamin D deficiency: a randomized double-blind controlled clinical trial.

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Moini, Ashraf; Ebrahimi, Tabandeh; Shirzad, Nooshin; Hosseini, Reihaneh; Radfar, Mania; Bandarian, Fatemeh; Jafari-Adli, Shahrzad; Qorbani, Mostafa; Hemmatabadi, Mahboobeh

2016-06-01

Dysmenorrhea is common among women of reproductive age. This study aim was to investigate the effect of vitamin D (vit D) supplementation in treatment of primary dysmenorrhea with vit D deficiency. A randomized double-blind placebo-controlled clinical trial was conducted on 60 women with primary dysmenorrhea and vit D deficiency referred to our clinic at Arash Women's Hospital from September 2013 to December 2014. Eligible women were randomly assigned into treatment and control groups (30 in each group). Individuals in the treatment group received 50 000 IU oral vit D and the control group received placebo weekly for eight weeks. After two months of treatment, there was a significant difference in serum vit D concentration between the two groups (p dysmenorrhea and vit D deficiency could improve pain intensity.

Alteração de taxa de filtração glomerular em pacientes hipertensos do município de Vitória-ES

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Cynthia Perin Passigatti

2014-07-01

Full Text Available Como monitorização da função renal, utiliza-se a estimativa da filtração glomerular (FG. Neste estudo, objetivou-se estimar a FG mediante a equação de Cockcroft-Gault em hipertensos atendidos no município de Vitória-ES. Estudo transversal de dados secundários com 754 hipertensos atendidos nas unidades de saúde, em 2009. Para análise estatística, utilizou-se o teste quiquadrado. Predominou o sexo feminino, excesso de peso e pressão arterial não controlada. A média de idade foi de 58,18 anos (±13,52 e da creatinina sérica (CrS 0,81mg/dl (±0,28. A prevalência da FG reduzida foi de 19% quando de 30 a 59ml/min e 1,6% quando de 29 a 15ml/min; e apresentou-se 15 vezes maior em idosos, 4,93 vezes maior naqueles com CrS elevada, 2,19 vezes nos hipertensos com baixo peso e 1,6 vez mais prevalente em homem. O estudo evidenciou a importância de monitorar a função renal com vistas à intervenção precoce e retardamento da perda da função renal em hipertensos.

Combination of omalizumab and bee venom immunotherapy: does it work?

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Yılmaz, İnsu; Bahçecioğlu, Sakine Nazik; Türk, Murat

2018-01-01

Bee venom immunotherapy (b-VIT) can be combined with omalizumab therapy in order to suppress systemic reactions developing due to b-VIT itself. Omalizumab acts as a premedication and gains time for the immunotherapy to develop its immunomodulatory effects. However, the combination of omalizumab and b-VIT is not always effective enough. Herein we present a patient in whom successful immunotherapy cannot be achieved with combination of omalizumab to b-VIT.

Corredores verdes urbanos: estudo da viabilidade de conexão das áreas verdes de Vitória

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Homero Marconi Penteado

2007-12-01

Full Text Available Grande parte dos profissionais de planejamento urbano tem buscado melhorar a qualidade de vida da população e a aplicação de princípios de sustentabilidade, que se refletem, na maioria dos casos, na busca de ampliação das áreas verdes, sejam na forma de parques, praças, arborização de vias ou mesmo incentivo aos jardins particulares. Esta pesquisa apresenta o resultado do estudo de viabilidade de criação de corredores verdes urbanos na cidade de Vitória, estabelecendo uma rede de conexões utilizando-se de sua malha viária, visando incrementar as condições ecológicas e paisagísticas urbanas e melhores condições de circulação de pedestres e ciclistas. Está alicerçada nos conceitos desenvolvidos pela ecologia da paisagem, baseada em fragmentos, corredores e matriz. A metodologia adotada foi aplicada em uma área teste, onde foram realizados levantamentos preliminares, mapeamento das áreas verdes, seleção de vias para implantação de corredores, avaliação dos possíveis corredores, inventário e análise das vias, diretrizes para estabelecimento dos corredores, ensaio projetual e criação de uma rede de corredores verdes. Dentre os principais resultados, verificou-se a possibilidade de criação de corredores com pequenas modificações nos perfis de vias que permitiriam a inserção de vegetação contínua, calçadas e ciclovias com consequências diretas (otimização das condições microclimáticas, melhoria na paisagem, atratividade para pequenos animais, incentivo ao percurso não motorizado, entre outros e indiretas (redução das partículas em suspensão, redução dos níveis de ruído e do consumo de combustíveis etc..

Histological and Biochemical Analyses of the Effects of Royal Jelly and Vitamin C against Phenylhydrazine-Induced Cardiotoxicity in Mice

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Hojat Anbara

2017-10-01

Full Text Available Abstract Background: Phenylhydrazine (PHZ as a strong oxidant agent causes variety of toxic effects including alterations in the biochemical and cardiac tissue. The aim of the present study was to investigate the effects of royal jelly (RJ and vitamin C (vit C against PHZ-induced cardiotoxicity in mice. Materials and Methods: Adult male mice were randomly assigned to eight groups of eight mice each. PHZ was administered to four groups of mice at a dose of 60 mg/kg per 48 hours intraperitoneally for 35 days. Three of these groups received vit C (250 mg/kg per day intraperitoneally, RJ (100 mg/kg per day orally and vit C+RJ with same doses four hours before PHZ administration, respectively. A vehicle-treated control group and vit C, RJ and vit C+RJ control groups were also included. Results: RJ and vit C significantly decreased (p< 0.05 the serum level of malondialdehyde and creatine kinase (CK-BM that had been increased by PHZ. Also, RJ and vit C increased the total antioxidant capacity and supraxoid dismutase serum that had been decreased by induced PHZ. Moreover, RJ and vit C could improve the tissue damages induced by PHZ such as diffused edema, hemorrhage, congestion, hyaline exudates, necrosis and also fibrosis tissue in heart tissue. Conclusion: It seems that Vit C and RJ can minimize PHZ-induced cardiotoxicity in mouse through oxidative reactions inhibition.

Changes in gene expression caused by insect venom immunotherapy responsible for the long-term protection of insect venom-allergic patients.

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Niedoszytko, Marek; Bruinenberg, Marcel; de Monchy, Jan; Weersma, Rinse K; Wijmenga, Cisca; Jassem, Ewa; Elberink, Joanne N G Oude

2011-06-01

Insect venom immunotherapy (VIT) is the only causative treatment of insect venom allergy (IVA). The immunological mechanism(s) responsible for long-term protection achieved by VIT are largely unknown. A better understanding is relevant for improving the diagnosis, prediction of anaphylaxis, and monitoring and simplifying treatment of IVA. To find genes that are differentially expressed during the maintenance phase of VIT and after stopping, to get clues about the pathways involved in the long-term protective effect of immunotherapy. Whole genome gene expression analysis was performed on RNA samples from 50 patients treated with VIT and 43 healthy controls. Patients were divided into three groups: (1) before the start of VIT; (2) on maintenance phase of VIT for at least 3 years still receiving injections; and (3) after VIT. Of all 48,804 probes present in the array, 48,773 transcripts had sufficient data for further analysis. The list of genes that were differentially expressed (at least log2 FC > 2; P Immunology. Published by Elsevier Inc. All rights reserved.

Prevalência de HIV em gestantes e transmissão vertical segundo perfil socioeconômico, Vitória, ES

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Anne Caroline Barbosa Cerqueira Vieira

2011-08-01

Full Text Available OBJETIVO: Descrever a prevalência de infecção por HIV em gestantes e a taxa de transmissão vertical, segundo o perfil socioeconômico dos bairros de residência das mães. MÉTODOS: Estudo ecológico exploratório utilizando a base de dados do Sistema de Informação de Agravos de Notificação de gestantes HIV-positivas e aids em crianças notificadas entre 2000 e 2006 em Vitória, ES. Para análise das informações socioeconômicas foi utilizado o Índice de Qualidade Urbana. A prevalência de HIV em gestantes e a taxa de transmissão vertical foram calculadas. A distribuição espacial dos casos foi realizada no programa Terraview 3.2.0. Para verificar a associação entre a qualidade urbana e a prevalência de HIV em gestantes utilizou-se o modelo de regressão de Poisson. RESULTADOS: Um total de 137 gestantes e 14 crianças infectadas por transmissão vertical foi notificado no período. Sete crianças correspondiam a mães HIV-positivas sem notificação de caso no período analisado. A prevalência de infecção em gestantes no período foi de 0,44% e a taxa de transmissão vertical foi de 9,7%. CONCLUSÕES: A prevalência de infecção por HIV em gestantes e a transmissão vertical associam-se à qualidade urbana do bairro de residência, indicando que os bairros com menor qualidade urbana devem ser priorizados quanto às ações para redução da transmissão vertical.

A preliminary study on the effects of star fruit consumption on antioxidant and lipid status in elderly Thai individuals

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Leelarungrayub J

2016-08-01

Full Text Available Jirakrit Leelarungrayub,1 Araya Yankai,1 Decha Pinkaew,1 Rungthip Puntumetakul,2 James J Laskin,3 Richard J Bloomer4 1Department of Physical Therapy, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, 2Research Center in Back, Neck, Other Joint Pain and Human Performance (BNOJPH, Khon Kaen University, Khon Kaen, Thailand; 3School of Physical Therapy and Rehabilitation Sciences, University of Montana, Missoula, MT, 4Cardiorespiratory/Metabolic Laboratory, School of Health Studies, University of Memphis, Memphis, TN, USA Objective: The aims of this preliminary study were to evaluate the antioxidant and lipid status before and after star fruit juice consumption in healthy elderly subjects, and the vitamins in star fruit extracts.Methods: A preliminary designated protocol was performed in 27 elderly individuals with a mean (±SD age of 69.5±5.3 years, by planning a 2-week control period before 4 weeks of consumption of star fruit twice daily. Oxidative stress parameters such as total antioxidant capacity, glutathione, malondialdehyde, protein hydroperoxide, multivitamins such as L-ascorbic acid (Vit C, retinoic acid (Vit A, and tocopherol (Vit E, and the lipid profile parameters such as cholesterol, triglyceride, high-density lipoprotein-cholesterol (HDL-C and low-density lipoprotein-cholesterol (LDL-C were analyzed. Moreover, Vit C, Vit A, and Vit E levels were evaluated in the star fruit extracts during the 4-week period.Results: In the 2-week control period, all parameters showed no statistically significant difference; after 4 weeks of consumption, significant improvement in the antioxidant status was observed with increased total antioxidant capacity and reduced malondialdehyde and protein hydroperoxide levels, as well as significantly increased levels of Vit C and Vit A, when compared to the two-time evaluation during the baseline periods. However, glutathione and Vit E showed no statistical difference. In addition

American Academy of Asthma, Allergy & Immunology membership experience with venom immunotherapy in chronic medical conditions and pregnancy, and in young children.

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Calabria, Christopher W; Hauswirth, David W; Rank, Matthew; Sher, Lawrence; Larenas-Linnemann, Desiree

2017-03-01

Few data exist regarding the use of venom immunotherapy (VIT) in specific high-risk chronic medical conditions and pregnancy, and in young children. A Web-based survey was sent to American Academy of Asthma Allergy & Immunology members to explore their VIT experience in potential high-risk medical conditions and pregnancy, and in young children. Major problems were defined as "activation of underlying disease and/or VIT not well tolerated (systemic adverse events) and/or VIT discontinued for medical reasons." Results were expressed descriptively. A total of 697 of 5123 surveys (14%) were completed: 87% of the respondents were based in the United States, and 28% worked in an academic setting. Most respondents (71%) believed that pregnancy was a contraindication for starting VIT. Most were comfortable continuing VIT (51%) if the woman became pregnant after starting therapy. Of the allergists who treated children, many would give VIT down to age 5 years (42%) or younger, ages 1-4 years (35%). The following list is of the specific medical condition, the number of allergists who used VIT in patients with this condition, and the percentage who reported major problems: severe asthma, 212 (4.2%); hypertension, 287 (1.1%); coronary artery disease, 222 (3.6%); arrhythmias, 136 (3.4%); cerebrovascular disease, 104 (5.1%); cancer in remission, 166 (0%); cancer stable but still under treatment, 44 (7.2%); a history of bone marrow transplantation, 15 (4.9%); a history of solid organ transplantation, 29 (3.6%); human immunodeficiency virus, 53 (1.4%); acquired immunodeficiency syndrome, 24 (6.2%); stable autoimmune disease, 164 (2.8%); mastocytosis, 66 (18.4%); elevated serum tryptase, 101 (10.8%); immunodeficiency 59 (2.5%). Many allergists were comfortable using VIT in young children and continuing but not starting pregnant women on VIT. VIT was commonly used in patients with hypertension, coronary artery disease, arrhythmias, cancer in remission, and stable autoimmune disease

Vitamin B-12 concentration, memory performance, and hippocampal structure in patients with mild cognitive impairment.

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Köbe, Theresa; Witte, A Veronica; Schnelle, Ariane; Grittner, Ulrike; Tesky, Valentina A; Pantel, Johannes; Schuchardt, Jan Philipp; Hahn, Andreas; Bohlken, Jens; Rujescu, Dan; Flöel, Agnes

2016-04-01

Low-normal concentrations of vitamin B-12 (VitB12) may be associated with worse cognition. However, previous evidence has been mixed, and the underlying mechanisms remain unclear. We determined whether serum VitB12 concentrations within the normal range were linked to memory functions and related neuronal structures in patients with mild cognitive impairment (MCI). In a cross-sectional design, we assessed 100 amnestic MCI patients (52 women; age range: 50-80 y) with low- and high-normal VitB12 concentration (median split: 304 pmol/L) for memory functions with the use of the Auditory Verbal Learning Test. MRI was performed at 3 tesla (n= 86) for the estimation of the volume and microstructure of the hippocampus and its subfields as indicated by the mean diffusivity on diffusion-weighted images. With the use of a mediation analysis, we examined whether the relation between VitB12 and memory performance was partially explained by volume or microstructure. MCI patients with low-normal VitB12 showed a significantly poorer learning ability (P= 0.014) and recognition performance (P= 0.008) than did patients with high-normal VitB12. Also, the microstructure integrity of the hippocampus was lower in patients with low-normal VitB12, mainly in the cornu ammonis 4 and dentate gyrus region (P= 0.029), which partially mediated the effect of VitB12 on memory performance (32-48%). Adjustments for age, sex, education, apolipoprotein E e4 status, and total homocysteine, folate, and creatinine did not attenuate the effects. Low VitB12 concentrations within the normal range are associated with poorer memory performance, which is an effect that is partially mediated by the reduced microstructural integrity of the hippocampus. Future interventional trials are needed to assess whether supplementation of VitB12 may improve cognition in MCI patients even in the absence of clinically manifested VitB12 deficiency. This trial was registered at clinicaltrials.gov as NCT01219244. © 2016

Administración oral de preparado parenteral de vitamina K en anticoagulación excesiva por warfarina Oral administration of intravenous preparation of Vitamin K for excessive anticoagulation due to warfarin

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Yoleima Lozada

2012-04-01

Full Text Available La warfarina es frecuentemente usada en la terapia anticoagulante actual, su acción debe ser monitorizada usando el tiempo de protrombina expresado como International Normalized Ratio (INR; cuando se excede el rango de seguridad se puede administrar vitamina K (Vit-K, preferentemente por vía oral. Dicha presentación no está disponible en Venezuela. Se realizó un ensayo clínico, doble ciego, donde a 20 pacientes, edad 18-60 años, sin sangrado e INR inicial de 6 a 10 inclusive; les fue suspendida la warfarina e inmediatamente agrupados al azar a recibir dosis única de Vit-K (oral 1.25mg de Vit-K fraccionada de una presentación parenteral o placebo. El punto final primario, INR Anticoagulation therapy with warfarin, a common clinical practice, needs to be monitored using protombine time expressed as the International Normalized Ratio (INR; when safety range is exceeded, Vitamin K (Vit-K could be administered with preference orally. In Venezuela the specific oral preparation for Vit-K is not available. This is a double blinded, randomized, placebo controlled, clinical trial; 20 patients, age 18-60 year with initial INR ≥ 6, ≤10, were randomized to oral Vit-K 1.25mg (prepared from intravenous presentation or placebo plus withholding warfarin. INR < 3.5 at 24 hours of treatment (the primary end point was achieved by 70% among Vit-K, and 20% among placebo patients; given an absolute risk reduction (ARR, of 50% (CI95%: 14.4-85.6 ρ = 0.028, NNT 2 (CI95%: 1.3 - 6.9. No adverse events were recorded including INR < 2 at 24 hours of treatment administration. Our results are consistent with studies where specific oral presentation of Vit-K was used. The results indicate that oral administration of Vit-K, prepared from an intravenous Vit-K preparation, is safe and more effective to revert excessive anticoagulation than simply withholding warfarin, in places where specific preparation of oral Vit-K is not available or too expensive.

L-ascorbate attenuates the endotoxin-induced production of inflammatory mediators by inhibiting MAPK activation and NF-κB translocation in cortical neurons/glia Cocultures.

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Ya-Ni Huang

Full Text Available In response to acute insults to the central nervous system, such as pathogen invasion or neuronal injuries, glial cells become activated and secrete inflammatory mediators such as nitric oxide (NO, cytokines, and chemokines. This neuroinflammation plays a crucial role in the pathophysiology of chronic neurodegenerative diseases. Endogenous ascorbate levels are significantly decreased among patients with septic encephalopathy. Using the bacterial endotoxin lipopolysaccharide (LPS to induce neuroinflammation in primary neuron/glia cocultures, we investigated how L-ascorbate (vitamin C; Vit. C affected neuroinflammation. LPS (100 ng/ml induced the expression of inducible NO synthase (iNOS and the production of NO, interleukin (IL-6, and macrophage inflammatory protein-2 (MIP-2/CXCL2 in a time-dependent manner; however, cotreatment with Vit. C (5 or 10 mM attenuated the LPS-induced iNOS expression and production of NO, IL-6, and MIP-2 production. The morphological features revealed after immunocytochemical staining confirmed that Vit. C suppressed LPS-induced astrocytic and microglial activation. Because Vit. C can be transported into neurons and glia via the sodium-dependent Vit. C transporter-2, we examined how Vit. C affected LPS-activated intracellular signaling in neuron/glia cocultures. The results indicated the increased activation (caused by phosphorylation of mitogen-activated protein kinases (MAPKs, such as p38 at 30 min and extracellular signal-regulated kinases (ERKs at 180 min after LPS treatment. The inhibition of p38 and ERK MAPK suppressed the LPS-induced production of inflammatory mediators. Vit. C also inhibited the LPS-induced activation of p38 and ERK. Combined treatments of Vit. C and the inhibitors of p38 and ERK yielded no additional inhibition compared with using the inhibitors alone, suggesting that Vit. C functions through the same signaling pathway (i.e., MAPK as these inhibitors. Vit. C also reduced LPS-induced Iκ

Maternal vitamin C deficiency does not reduce hippocampal volume and beta-tubulin III intensity in prenatal Guinea pigs

DEFF Research Database (Denmark)

Hansen, Stine Normann; Schjoldager, Janne Gram; Paidi, Maya Devi

2016-01-01

Marginal vitamin C (vitC) deficiency affects 5% to 10% of adults including subpopulations such as pregnant women and newborns. Animal studies link vitC deficiency to deleterious effects on the developing brain, but exactly how the brain adapts to vitC deficiency and the mechanisms behind...... the observed deficits remain largely unknown. We hypothesized that vitC deficiency in utero may lead to a decreased neuronal maturation and increased cellular death giving rise to alterations of the hippocampal morphology in a guinea pig model. Brains from prenatal guinea pig pups (n = 9-10 in each group......) subjected to either a sufficient (918 mg vitC/kg feed) or deficient (100 mg vitC/kg feed) maternal dietary regimen were assessed with regards to hippocampal volume and beta-tubulin isotype III staining intensity at 2 gestational time points (45 and 56). We found a distinct differential regional growth...

1,25-dihydroxyvitamin D3 is an autonomous regulator of the transcriptional changes leading to a tolerogenic dendritic cell phenotype.

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Széles, Lajos; Keresztes, Gábor; Töröcsik, Dániel; Balajthy, Zoltán; Krenács, László; Póliska, Szilárd; Steinmeyer, Andreas; Zuegel, Ulrich; Pruenster, Monika; Rot, Antal; Nagy, László

2009-02-15

Activation of vitamin D receptor (VDR) by 1,25-dihydroxyvitamin D(3) (1,25-vitD) reprograms dendritic cells (DC) to become tolerogenic. Previous studies suggested that 1,25-vitD could inhibit the changes brought about by differentiation and maturation of DCs. Underpinning the described phenotypic and functional alterations, there must be 1,25-vitD-coordinated transcriptional events. However, this transcriptional program has not been systematically investigated, particularly not in a developmental context. Hence, it has not been explored how 1,25-vitD-regulated genes, particularly the ones bringing about the tolerogenic phenotype, are connected to differentiation. We conducted global gene expression analysis followed by comprehensive quantitative PCR validation to clarify the interrelationship between 1,25-vitD and differentiation-driven gene expression patterns in developing human monocyte-derived and blood myeloid DCs. In this study we show that 1,25-vitD regulates a large set of genes that are not affected by differentiation. Interestingly, several genes, impacted both by the ligand and by differentiation, appear to be regulated by 1,25-vitD independently of the developmental context. We have also characterized the kinetics of generation of 1,25-vitD by using three early and robustly regulated genes, the chemokine CCL22, the inhibitory receptors CD300LF and CYP24A1. We found that monocyte-derived DCs are able to turn on 1,25-vitD sensitive genes in early phases of differentiation if the precursor is present. Our data collectively suggest that exogenous or endogenously generated 1,25-vitD regulates a large set of its targets autonomously and not via inhibition of differentiation and maturation, leading to the previously characterized tolerogenic state.

Dietary Effect of Selenium-enriched Radish Sprouts, Vitamin E, and Rhodobacter capsulatus on Hypocholesterolemia and Immunity of Broiler

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Tsujii H

2017-06-01

Full Text Available The study was designed to investigate the effects of dietary Selenium-enriched radish sprouts (Se-RS, Vitamin E (Vit E, and Rhodobacter capsulatus (RC on immunity, cholesterol concentration, and fatty acid composition in broiler meat. A total of 100 two-week-old male broiler chicks were randomly assigned into five dietary groups: I Control; II Se-RS (5 μg/kg Se-RS; III Se-RS+RC (5 μg/kg Se-RS + 0.2 g/kg RC; IV Se-RS+Vit E (5 μg/kg Se-RS + 50 mg/kg Vit E and V Se-RS+RC+Vit E (5 μg/kg Se-RS + 0.2 g/kg RC + 50 mg/kg Vit E. Diets and clean drinking water were offered ad libitum. After the end of 3-wk of feeding period, serum cholesterol and triglycerides concentrations were lower (P < 0.05 in broilers fed Se-RS + RC + Vit E supplemented diet compared to the control diet. At the end of the 6-wk feeding period, birds fed the Se-RS+RC+Vit E diet significantly (P < 0.05 reduced cholesterol and triglycerides concentrations and improved the ratio of unsaturated fatty acids to saturated fatty acids in broiler meat. The highest (P < 0.05 number of leukocytes was observed in broilers fed Se-RS+RC+Vit E supplemented diet. Foot web index and weights of spleen, bursa, and thymus were significantly (P < 0.05 higher in birds fed Se-RS+RC+Vit E compared to the control diet. Our findings suggest that there are dual benefits of supplementing broiler diets with Se-RS+RC+Vit E because of improvements in the bird’s immunity and meat quality that is important for health conscious consumers.

Vitamin D Status and Related Factors in Newborns in Shanghai, China

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Xiaodan Yu

2014-12-01

Full Text Available With the increasing recognition of the importance of the non-skeletal effects of vitamin D (VitD, more and more attention has been drawn to VitD status in early life. However, the VitD status of newborns and factors that influence VitD levels in Shanghai, China, remain unclear. A total of 1030 pregnant women were selected from two hospitals in Shanghai, one of the largest cities in China located at 31 degrees north latitude. Umbilical cord serum concentrations of 25-hydroxy vitamin D [25(OHD] were measured by LC-MS-MS, and questionnaires were used to collect information. The median cord serum 25(OHD concentration was 22.4 ng/mL; the concentration lower than 20 ng/mL accounted for 36.3% of the participants, and the concentration lower than 30 ng/mL for 84.1%. A multivariable logistic regression model showed that the determinants of low 25(OHD status were being born during autumn or winter months and a lack of VitD-related multivitamin supplementation. The relative risk was 1.7 for both autumn (95% CI, 1.1–2.6 and winter (95% CI, 1.1–2.5 births (p < 0.05. VitD-related multivitamin supplementation more than once a day during pregnancy reduced the risk of VitD deficiency [adjusted OR (aOR = 0.6, 95% CI (0.45–1.0 for VitD supplementation] (p < 0.05. VitD deficiency and insufficiency are common in newborns in Shanghai, China, and are independently associated with season and VitD supplementation. Our findings may assist future efforts to correct low levels of 25(OHD in Shanghai mothers and their newborn children.

A 13-year real-life study on efficacy, safety and biological effects of Vespula venom immunotherapy.

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Albanesi, Marcello; Nico, Andrea; Sinisi, Alessandro; Giliberti, Lucia; Rossi, Maria Pia; Rossini, Margherita; Kourtis, Georgios; Rucco, Anna Simona; Loconte, Filomena; Muolo, Loredana; Zurlo, Marco; Di Bona, Danilo; Caiaffa, Maria Filomena; Macchia, Luigi

2018-01-01

Hymenoptera venom immunotherapy (VIT) is a clinically effective treatment. However, little is known about its long-term clinical efficacy and biological effects. Several mechanisms have been proposed to account for VIT efficacy, including reduction of specific IgE and induction of allergen-specific IgG 4 , but the overall picture remains elusive. We investigated Vespula VIT clinical efficacy up to 8 years after discontinuation and the kinetics of Vespula -specific IgE and IgG 4 . Out of 686 consecutive patients we retrospectively selected and analysed a series of 23 patients with Vespula allergy that underwent a 5-year IT course, followed by a prolonged follow-up. Clinical efficacy of VIT was assessed as number and severity of reactions to Vespula re-stinging events. The presence of Vespula -specific IgE and IgG 4 was also monitored over time. During the VIT treatment, patients were protected, reporting no reactions or mild reactions in occasion of re-stinging events. This protection was entirely maintained during the follow-up, up to 8 years. Skin reactivity (reflecting mast cell-bound Vespula -specific IgE) and circulating Vespula -specific IgE levels declined substantially during VIT. Notably, this reduction was maintained over time during the follow-up. Moreover, all the patients were analysed for IgG 4 . A robust induction of Vespula -specific IgG 4 was observed during the VIT course, with a substantial decline during the follow-up. We conclude that Vespula VIT is a clinically effective treatment, which induces long-term protection after discontinuation. The reduction of specific IgE, assessed by skin tests and RAST, closely matches the VIT- induced protection, while the IgG 4 induction seems not to be associated with VIT clinical efficacy in the long term.

Sostenibles legislación: directrices para incorporar los conceptos de sostenibilidad en el código de edifícios en Vitória/ES

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Érika da Cunha Victor Souza

2015-12-01

Full Text Available This article aims to examine the legal aspects of sustainable construction proposing guidelines for incorporating sustainability concepts in the Code of public works and construction of the Vitória-ES. In the global context, sustainability is evident, reinforcing the concern about the impact generated by man to the environment directly affecting the economy, society and the planet’s future, so the importance of environmental preservation and the efficient use of natural resources (water, air, energy, materials etc.. In this sense the role of legislation is important, since the goal of the code works in the municipalities is to ensure minimum conditions of environmental comfort in the deployment of any building to be built in the city. The hypothesis is that the legislation needs to be revised and the goal is to adjust it by inserting sustainability concepts. The methodology is based on literature search applied to the subject. We conclude that the Government should contribute more effectively to the reconstruction of the cities scene, playing their role through legal instruments, more effective and active way for both construction of new buildings, the renovation and adaptation of existing ones.

Seminal quality and sperm production in beef bulls with chronic dietary vitamin A deficiency and subsequent re-alimentation.

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Rode, L M; Coulter, G H; Kastelic, J P; Bailey, D R

1995-05-01

Sixteen Hereford bulls (16 mo of age, 462 kg average body weight) were used in each of 2 yr to evaluate the effects of hypovitaminosis A on seminal quality and sperm production. Bulls were fed a high-concentrate diet with (+VIT) or without (-VIT) supplemental Vitamin A until the apparent onset of hypovitaminosis A (28 and 32 wk in Year 1 and 2, respectively). Half of the bulls on each treatment were then slaughtered and those remaining were re-alimented with Vitamin A. Plasma retinol concentration in -VIT bulls reached a nadir at approximately 25 wk. In Year 1, the proportion of progressively motile spermatozoa was lower in -VIT bulls after 17 wk but returned to that of the +VIT group after re-alimentation. The proportion of spermatozoa with primary morphological defects appeared to be greater in -VIT bulls compared to +VIT bulls by 26 and 24 wk in Year 1 and 2, respectively. The incidence of these defects declined in -VIT bulls upon re-alimentation, and approached the incidence observed in +VIT bulls by 8 to 12 wk of re-alimentation. Hypovitaminosis A decreased paired testes weight, daily sperm production, and epididymal sperm reserves but did not affect daily gain. Prolonged dietary Vitamin A deficiency impaired semen quality and sperm production in the absence of other clinical symptoms. However, under practical feeding conditions, diets that result in long-term, marginal Vitamin A deficiency or a relatively short-term absence of Vitamin A intake probably would have minimal effects on spermatogenesis.

Maternal vitamin C deficiency during pregnancy persistently impairs hippocampal neurogenesis in offspring of guinea pigs.

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Pernille Tveden-Nyborg

Full Text Available While having the highest vitamin C (VitC concentrations in the body, specific functions of VitC in the brain have only recently been acknowledged. We have shown that postnatal VitC deficiency in guinea pigs causes impairment of hippocampal memory function and leads to 30% less neurons. This study investigates how prenatal VitC deficiency affects postnatal hippocampal development and if any such effect can be reversed by postnatal VitC repletion. Eighty pregnant Dunkin Hartley guinea pig dams were randomized into weight stratified groups receiving High (900 mg or Low (100 mg VitC per kg diet. Newborn pups (n = 157 were randomized into a total of four postnatal feeding regimens: High/High (Control; High/Low (Depleted, Low/Low (Deficient; and Low/High (Repleted. Proliferation and migration of newborn cells in the dentate gyrus was assessed by BrdU labeling and hippocampal volumes were determined by stereology. Prenatal VitC deficiency resulted in a significant reduction in postnatal hippocampal volume (P<0.001 which was not reversed by postnatal repletion. There was no difference in postnatal cellular proliferation and survival rates in the hippocampus between dietary groups, however, migration of newborn cells into the granular layer of the hippocampus dentate gyrus was significantly reduced in prenatally deficient animals (P<0.01. We conclude that a prenatal VitC deficiency in guinea pigs leads to persistent impairment of postnatal hippocampal development which is not alleviated by postnatal repletion. Our findings place attention on a yet unrecognized consequence of marginal VitC deficiency during pregnancy.

Radioprotective effect of both vitamins E and / or C on some blood components of male mice

International Nuclear Information System (INIS)

Anwar, W.A.; El-Daway, H.A.E.; Tawfik, S.S.M.; Soliman, S.M.

1999-01-01

The protective effects of vitamin C and/or vitamin E on some blood components and serum level of thiobarbituric reactive substances (as indicator to level of lipid peroxidation) of irradiated male mice at dose 3 Gy sacrificed after 15,24 and 46 days of irradiation have been assessed in comparison with control and non-irradiated-treated with vitamin C and/or vitamin E groups. Vit. C, vit. E or in combination decreased the effect of irradiation on blood components and the level of thiobarbituric reactive substances, but average haemoglobin level RBC count, total WBC count, neutrophil count and lymphocyte count did not reach to the original average level even after 36 days of irradiation. Vit. E had a more protective effect than vit. C in case of total white blood cells, while vit. C was more protective in case of lymphocyte count. The combination of vitamins C and E had more protective effect than either of both vitamins alone. The decrease in lipid peroxidation products due to administration of vit. E and vit. C as antioxidants supported the theory that lipid peroxidation increased due to free radicals induced by irradiation

Calcium vitamin D3 supplementation in clinical practice: side effect and satisfaction.

Science.gov (United States)

Sanaei, Maryam; Banasiri, Mohammad; Shafiee, Gita; Rostami, Mahsa; Alizad, Saba; Ebrahimi, Mehdi; Larijani, Bagher; Heshmat, Ramin

2015-01-01

The objective of this study was to assess side effects and satisfaction about OsteoCalVitFort (500 mg calcium and 400 I.U. of vitamin D3) usage. A total 186 people were participated with range age from 18 to 65 years old. Each participant received 1 pack that contains 60 OsteoCalVitFort tablet and used two tablet OsteoCalVitFort daily (1 tablet after breakfast and 1 after dinner). By a phone call, side effects and satisfaction about OsteoCalVitFort were assessed. The rate of constipating (8.0 %) and bloating (12.5 %) were decreased significantly after OsteoCalVitFort supplement intake (1.2 %, and 0.6 %, respectively). Similar results were observed in metallic taste in mouth, tiredness, weakness, loss of appetite, bone/muscle pain and mental/mood change after Calcium Vitamin D3 supplementation intake. Totally, 94 % of patients were satisfied about OsteoCalVitFort usage. The results of the research indicate despite the high quality of OsteoCalVitFort supplement, there are no side effects which have been seen in other supplements.

Influence of hesperidin and vitamin C on glycemic parameters, lipid profile, and DNA damage in rats treated with sucrose overload

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SILVIA I.R. FRANKE

2018-04-01

Full Text Available ABSTRACT We evaluated the influence of hesperidin and vitamin C (VitC on glycemic parameters, lipid profile, and DNA damage in male Wistar rats treated with sucrose overload. Rats were divided into six experimental groups: I-water control; II-sucrose control; III-hesperidin control; IV-VitC control; V-co-treatment of sucrose plus hesperidin; VI-co-treatment of sucrose plus VitC. We measured the levels of triglycerides, total cholesterol, HDL-c, LDL-c, fasting glucose, and glycated hemoglobin (A1C. DNA damage was evaluated in blood and brain cells using the comet assay and the micronucleus test was used to evaluate chromosomal damages in the rat bone marrow. Co-treatment with VitC, but not with hesperidin, normalized the serum glucose. No effect of co-treatments was observed on A1C. The co-treatment with VitC or hesperidin did not influence the lipid profile (p>0.05. Rats co-treated with hesperidin had a significantly lower DNA damage level in blood (p0.05. Hesperidin and VitC showed different effects on sucrose and DNA damage levels. While VitC lowered the serum glucose, hesperidin reduced the DNA damage.

Variabilidade espacial de atributos de um solo sob videira em Vitória Brasil (SP Spatial variability of soil characteristics under grapevine in Vitoria Brasil (State of Sao Paulo - Brazil

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M. P. Carvalho

2003-08-01

Full Text Available Foi pesquisada a variabilidade espacial de alguns atributos físicos e químicos de uma associação de solos cultivada sob videira (Vitis vinifera-L, do município de Vitória Brasil, estado de São Paulo, Brasil. O objetivo foi estudar a dependência espacial de tais atributos, assim como caracterizar as respectivas variabilidades, distribuições de freqüência e números mínimos de subamostras do solo para a cultura da videira. Para isso, coletaram-se dados do solo, dispostos segundo uma malha com 156 pontos amostrais, sendo analisados por meio da geoestatística. As maiores variabilidades foram verificadas para a macroporosidade (MA, P, K, Ca, Mg, SB e CTC, ao passo que as menores foram para a densidade do solo (DS, pH e V. O número mínimo de subamostras, necessário para formar uma amostra composta e representativa, variou de 1 (pH e V a 241 (Mg, tendo seu valor médio de 64 subamostras. Quanto à dependência espacial, o P e o V apresentaram, respectivamente, forte e fraca dependência, enquanto o restante dos atributos apresentou moderada dependência. Desta forma, o alcance dos atributos físicos variou de 2,56 a 4,32 m, enquanto o dos químicos variou de 1,82 a 5,64 m.The spatial variability of some physical and chemical characteristics of a compound of soils under grapevine (Vitis vinifera-L cultivation was studied in the county Vitória Brasil, State of Sao Paulo, Brazil. Main objective was research into the spatial dependence of these soil characteristics and their variability, frequency distribution and minimum number of soil subsamples for grapevine crop. Soil data were collected in grid sampling at 156 points, using geoestatistics for the data analysis. Highest variability was found for macroporosity (MA, P, K, Ca, Mg, SB, and CTC, and the smallest for bulk density (BD, pH, and V. The minimum number of soil subsamples to form a compound and representative sample varied between 1 (pH and V and 241 (Mg, with a mean of 64

Emerging role of Vitamin D in health

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Deepanjali Lomte

2014-03-01

Full Text Available Vit. D is a steroid prohormone. It is synthesized in the skin under ultra –violet light exposure. Traditionally, Vit.D3, cholecalciferol and calcitriol were known to have a role only in calcium and bone mineral metabolism. This article takes an account of the current view on the impact of Vit D deficiency on human health. Research has now shown the indisputable role of Vit D in prevention/treatment of some cancers, osteoporosis, rheumatoid arthritis, multiple sclerosis, tuberculosis, hypertension, and diabetes mellitus. In addition lower maternal Vit. D levels are associated with Pregnancy Induced Hypertension (PIH, suggesting that Vit. D deficiency may be a modifiable risk factor for PIH. Greater awareness of the problem of a high prevalence of vitamin D inadequacy is required among researchers, clinicians, and patients. Special efforts on the medical and social fronts are nec­essary to combat this preventable epidemic of vitamin D deficiency.

Consumo de medicamentos por estudantes adolescentes de Escola de Ensino Fundamental do município de Vitória

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M. A. ANDRADE

2009-07-01

Full Text Available O uso indiscriminado de medicamentos acarreta riscos diretos e indiretos à população, tornando-se um importante problema de saúde pública. O conhecimento do padrão de uso de medicamentos contribui para ações educativas com a finalidade de reduzir riscos e danos à saúde da população. O objetivo deste estudo foi investigar o perfil de consumo de medicamentos em estudantes adolescentes e correlacioná-lo com o perfil sócio-educacional entre os familiares. Estudo de natureza transversal com amostra representativa de 102 alunos da população de escolares de ensino médio no Bairro Maruípe em Vitória-ES. O questionário aplicado contemplou as variáveis: idade, gênero, série, consumo de medicamentos nos sete dias anteriores à entrevista, quem orientou o consumo e a automedicação, aos quais foram avaliados por meio de perguntas dicotômicas, em caso afirmativo, especificando o(s fármaco(s utilizado(s e sua finalidade. O uso de medicamentos ocorreu em 42,3% da amostra, sendo que em 53,3% dos casos houve automedicação. Os grupos farmacológicos mais consumidos foram analgésicos/antiinflamatórios, sendo o paracetamol a substância com maior freqüência de consumo. A incidência de automedicação no grupo representado pelo gênero feminino (66,7% foi superior ao grupo do gênero masculino (33,3%. A relação entre a idade e automedicação mostrou uma maior prevalência entre os jovens de 15 a 17 anos de idade, representando 70,8% dos adolescentes que fazem automedicação. O consumo de medicamentos sem orientação médica pelos familiares mostrou ser um fator de influência na automedicação dos adolescentes. Palavras-chave: Adolescentes. Automedicação. Consumo de medicamentos.

Transients and cooperative action of β-carotine, vitamine E and vitamine C in biological systems in vitro under irradiation

International Nuclear Information System (INIS)

Getof, N.; Platzer, I.; Winkelbauer, C.

1998-01-01

Complete text of publication follows. In the scope of clinical studies in the USA it has been established that β-carotine (β car) and vitamine A (vit A; retinol) give rise to lung cancer and cardiovascular diseases on humans. The consumption of vitamine E (vit E) and β-carotine provokes lung cancer and other types of tumors on male smokers. This effect increases even significantly by a simultaneous consumption of alcohol. In contrary to these results there are other scientists, who did not observe any increase of the rate of lung cancer or other tumors by the consumption of β-car or vit E. Based on these contradictory statements experiments following two pathways were performed: Pulse radiolysis studies on radical cations and radical anions of β-car and vit E Investigations on baeteria (E. coli AB 1157) and cell cultures (SCC VII): their survival was studied as a function of the absorbed dose in the abscence and in the presence of the above mentioned vitamines and vitamine C (vit C). From our extensive studies we obtain following conclusions: - Metabolic changes in normal cells could probably be initiated by the radical cation of β-carotine (β-car '+) resulting from the action of β-car as an antioxidant. - Vitamine E can repair β-car '+ by electron transfer, forming the radical cation of vit E (vit E '+), whose biological action is yet unknown. - Vitamine C (ascorbate) is able to repair both, the β-caz'+ and the vit E'+ by electron transfer (cascade electron transfer), resulting in ascorbate radical, which can disproportionate to vit C and dehydroascorbic acid (DHA). The last one can be converted again enzymatically into ascorbic acid

Vitamin C Deficiency Reduces Muscarinic Receptor Coronary Artery Vasoconstriction and Plasma Tetrahydrobiopterin Concentration in Guinea Pigs

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Gry Freja Skovsted

2017-07-01

Full Text Available Vitamin C (vitC deficiency is associated with increased cardiovascular disease risk, but its specific interplay with arteriolar function is unclear. This study investigates the effect of vitC deficiency in guinea pigs on plasma biopterin status and the vasomotor responses in coronary arteries exposed to vasoconstrictor/-dilator agents. Dunkin Hartley female guinea pigs (n = 32 were randomized to high (1500 mg/kg diet or low (0 to 50 mg/kg diet vitC for 10–12 weeks. At euthanasia, coronary artery segments were dissected and mounted in a wire-myograph. Vasomotor responses to potassium, carbachol, sodium nitroprusside (SNP, U46619, sarafotoxin 6c (S6c and endothelin-1 (ET-1 were recorded. Plasma vitC and tetrahydrobiopterin were measured by HPLC. Plasma vitC status reflected the diets with deficient animals displaying reduced tetrahydrobiopterin. Vasoconstrictor responses to carbachol were significantly decreased in vitC deficient coronary arteries independent of their general vasoconstrictor/vasodilator capacity (p < 0.001. Moreover, in vitC deficient animals, carbachol-induced vasodilator responses correlated with coronary artery diameter (p < 0.001. Inhibition of cyclooxygenases with indomethacin increased carbachol-induced vasoconstriction, suggesting an augmented carbachol-induced release of vasodilator prostanoids. Atropine abolished carbachol-induced vasomotion, supporting a specific muscarinic receptor effect. Arterial responses to SNP, potassium, S6c, U46619 and ET-1 were unaffected by vitC status. The study shows that vitC deficiency decreases tetrahydrobiopterin concentrations and muscarinic receptor mediated contraction in coronary arteries. This attenuated vasoconstrictor response may be linked to altered production of vasoactive arachidonic acid metabolites and reduced muscarinic receptor expression/signaling.

RESÍDUOS SÓLIDOS: diagnóstico e proposições para o bairro de Redenção, no município de Vitória de Santo Antão/PE

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David dos Santos Azevedo

2016-08-01

Los residuos sólidos generados por las diversas actividades urbanas se presentan como una de las principales preocupaciones de la sociedad, a la vista de las consecuencias perjudiciales que pueden causar al medio ambiente cuando no se manejan correctamente. Hoy en día, es muy común ver a los grandes volúmenes de residuos sólidos en terrenos baldíos, los cursos de agua e incluso lugares públicos como aceras y plazas. El artículo tiene como objetivo realizar un diagnóstico sobre la eliminación incorrecta de los residuos sólidos en el barrio de Redenção, municipio de Vitória de Santo Antão-PE, ya partir de ese diagnóstico hacen propuestas para la educación ambiental y un sistema de recogida selectiva que hacen posible la correcta gestión de los residuos sólidos allí. Para ello, se realizaron visitas a la zona de la Redenção, hicieron registros fotográficos y descriptivos para destacar los puntos críticos de los residuos, el momento de la recogida convencional, los principales generadores de residuos y la disposición inadecuada de los residuos en esa comunidad. Los principales problemas observados fueron: construcción deposición de residuos en las carreteras locales, eliminación de residuos domésticos en los canales de agua de lluvia, los animales vagan cerca de acumulación de puntos críticos, la eliminación de desechos peligrosos terrenos baldíos. Frente a este diagnóstico se propusieron acciones de educación ambiental que implican comerciantes, escuelas y guarderías, líderes comunitarios y otros residentes. Teniendo en cuenta lo anterior, se puede concluir que existe una urgencia en extensión a la comunidad y el gobierno local, de modo que proporciona una mejor calidad de vida de la población y hacer cumplir las que determinan las políticas federales y estatales de los residuos sólidos. Palabras clave: Gestión de Residuos Sólidos; Educación Ambiental; Recogida Selectiva; Redencão; Vitória de Santo Antão/PE.

Maternal vitamin C deficiency during pregnancy persistently impairs hippocampal neurogenesis in offspring of guinea pigs

DEFF Research Database (Denmark)

Tveden-Nyborg, Pernille; Vogt, Lucile; Schjoldager, Janne Gram

2012-01-01

While having the highest vitamin C (VitC) concentrations in the body, specific functions of VitC in the brain have only recently been acknowledged. We have shown that postnatal VitC deficiency in guinea pigs causes impairment of hippocampal memory function and leads to 30% less neurons. This study...

Investigating the potential role of vitamin E in modulating the immunosuppressive effects of tylvalosin and florfenicol in broiler chickens.

Science.gov (United States)

El-Ela, Fatma I Abo; Shany, S A S; El-Deen, Manal B; El-Banna, H A; El-Gendy, A A; Hendy, K; Tohamy, M A

2016-10-01

Tylvalosin (TVS) is a third-generation macrolide drug used for prophylaxis and treatment of mycoplasma, however; it is supposed to possess an immunosuppressive effect. In the current study, the immunosuppressive effect of TVS and florfenicol (FFC) and the potential immunomodulatory role of Vit E were investigated. The experiment included one day old chick groups treated with either TVS, FFC, Vit E, TVS/Vit E, FFC/Vit E and control non-treated group. Chicks were vaccinated with inactivated H9N2 avian influenza (AI) vaccine and humoral antibody titers to viral antigen as well as innate immunity (serum lysozyme activity and nitric oxide levels) were evaluated. Total and differential leucocytic counts, serum liver enzymes level, blood leucocytic DNA damage and cellular area percentages within the lymphoid organs were also screened. Treatment with TVS and FFC significantly decreased immune response of chickens while treatment with Vit E improved the humoral immune response at 4 and 5weeks post-vaccination. Vit E also significantly increased the cellular immune response. The combination of Vit E with either TVS or FFC modulated their immunosuppressive effect and resulted in mild immunostimulatory effects. TVS alone induced a genotoxic effect on chickens' blood leucocytes and the genotoxicity was inhibited by combination of TVS with Vit E. Histopathology revealed that chickens treated with either TVS or FFC exhibited toxic effect on the lymphatic tissues. Copyright © 2016 Elsevier Ltd. All rights reserved.

The Role of Vitamin D Level and Related Single Nucleotide Polymorphisms in Crohn’s Disease

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Wen J. Lam

2013-09-01

Full Text Available New Zealand has one of the highest rates of Crohn’s Disease (CD in the world, and there is much speculation as to why this might be. A high risk of CD has been associated with deficient or insufficient levels of Vitamin D (Vit D, lifestyle as well as various genetic polymorphisms. In this study we sought to analyse the relevance of serum Vit D levels, lifestyle and genotype to CD status. Serum samples were analysed for 25-OH-Vitamin D levels. DNA was isolated from blood and cheek-swabs, and Sequenom and ImmunoChip techniques were used for genotyping. Serum Vit D levels were significantly lower in CD patients (mean = 49.5 mg/L than those found in controls (mean = 58.9 mg/L, p = 4.74 × 10−6. A total of seven single nucleotide polymorphisms were examined for effects on serum Vit D levels, with adjustment for confounding variables. Two variants: rs731236[A] (VDR and rs732594[A] (SCUBE3 showed a significant association with serum Vit D levels in CD patients. Four variants: rs7975232[A] (VDR, rs732594[A] (SCUBE3, and rs2980[T] and rs2981[A] (PHF-11 showed a significant association with serum Vit D levels in the control group. This study demonstrates a significant interaction between Vit D levels and CD susceptibility, as well as a significant association between Vit D levels and genotype.

Vitamin D, parathyroid hormone and cardiovascular risk: the good, the bad and the ugly.

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Pascale, Antonietta V; Finelli, Rosa; Giannotti, Rocco; Visco, Valeria; Fabbricatore, Davide; Matula, Ida; Mazzeo, Pietro; Ragosa, Nicola; Massari, Angelo; Izzo, Raffaele; Coscioni, Enrico; Illario, Maddalena; Ciccarelli, Michele; Trimarco, Bruno; Iaccarino, Guido

2018-02-01

: 25-Hydroxyvitamin D insufficiency and increased cardiovascular risk (CVR) association is still debated. The vitamin D (VitD)-dependent parathyroid hormone (PTH) is considered as the possible actuator of VitD effects on CVR. To investigate the association of CVR, PTH and VitD, we carried out blood pressure measurements and blood samples and collected information on dietary habits, anamnestic, clinical and metabolic data of 451 participants in the Salerno area (Southern Italy) during the World Hypertension Day (17 May). CVR was calculated according to the Framingham CVR charts. The overall population mean age was 51.6 ± 0.7 years, and female sex was slightly prevalent (55%). VitD deficiency (<20 ng/ml) was most frequent (59.7%). In this population, VitD and CVR did not correlate. VitD and PTH inversely correlated (r = -0.265, P < 0.001) as expected. PTH was in direct correlation (r = 0.225, P < 0.001) with CVR. Elevated PTH (75 percentile; ≥49.5 pg/ml) levels identify a population with higher CVR (11.8 ± 0.5 vs. 8.5 ± 0.3, P < 0.001). In a multivariate analysis, both age and PTH correlate to CVR, but not VitD. In conclusion, VitD does not directly affect CVR in the overall population. Rather, increased PTH might be a better predictor of CVR.

Calcium vitamin D3 supplementation in clinical practice: side effect and satisfaction

OpenAIRE

Sanaei, Maryam; Banasiri, Mohammad; Shafiee, Gita; Rostami, Mahsa; Alizad, Saba; Ebrahimi, Mehdi; Larijani, Bagher; Heshmat, Ramin

2016-01-01

Background The objective of this study was to assess side effects and satisfaction about OsteoCalVitFort (500?mg calcium and 400 I.U. of vitamin D3) usage. Methods A total 186 people were participated with range age from 18 to 65?years old. Each participant received 1 pack that contains 60 OsteoCalVitFort tablet and used two tablet OsteoCalVitFort daily (1 tablet after breakfast and 1 after dinner). By a phone call, side effects and satisfaction about OsteoCalVitFort were assessed. Results Th...

Differences in response to conventional vitamin D therapy among obese and normal weight children and adolescents in Qazvin, Iran

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Fatemeh Saffari

2017-09-01

Full Text Available Background: Vitamin D (Vit D deficiency is one of the major nutritional deficiencies in children. Obesity has inverse association with vitamin D levels. The aim of this study was to determine the differences in response to conventional treatment for Vit D deficiency and insufficiency in obese and normal weight children and adolescents.Methods: This nested case control study was conducted in 69 obese children and 133 normal weight matched control suffering from Vit D insufficiency or deficiency. Vit D deficiency was defined as serum 25(OH D

Effect of antioxidants on the dentin interface bond stability of adhesives exposed to hydrolytic degradation.

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Gotti, Valéria B; Feitosa, Victor P; Sauro, Salvatore; Correr-Sobrinho, Lourenço; Leal, Fernanda B; Stansbury, Jeffrey W; Correr, Américo B

2015-02-01

This study assessed the effect of antioxidants vitamin C (Vit. C), vitamin E (Vit. E) and quercetin (Querc) on the dentin bonding performance, degree of conversion, and rate of polymerization of three commercial adhesive systems (Adper Single Bond 2 [SB], Clearfil SE Bond [CSE], Adper Easy Bond [EB]). Human premolars were restored using antioxidant-doped adhesives. The samples were stored for 24 h in distilled water or 6 months under simulated pulpal pressure. Teeth were cut into sticks and the microtensile bond strength (μTBS) to dentin was tested in a universal testing machine. Qualitative nanoleakage analysis was performed from a central stick of each restored tooth. Degree of conversion and rate of polymerization of adhesive systems were evaluated in triplicate using real-time FT-IR. Although the inclusion of the antioxidants negatively affected the μTBS over 24 h, the antioxidant-doped adhesives maintained (SB-Vit. C, SB-Vit. E, CSE-Vit. C, EB-Querc) or increased (SB-Querc, CSE-Vit. E, CSE-Querc, EB-Vit. E, and EB-Vit. C) their μTBS during 6 months of storage. Only the μTBS of Adper Single Bond 2 dropped significantly after 6 months among the control groups. Slight changes in the nanoleakage pattern after aging were observed in all groups, except for the EB-control group, which showed a noteworthy increase in nanoleakage after 6 months, and for EB-Vit. C, which presented a remarkable decrease. A lower degree of conversion was obtained with all antioxidants in SB and EB, except for the EB-Vit. E group. Similar degrees of conversion were attained in control and experimental groups for CSE. The rate of polymerization was reduced in antioxidant-doped adhesives. The performance of antioxidants changed according to the adhesive system to which they were added, and antioxidant-doped adhesives appear to have a positive effect on the adhesive interface durability, since their bond strength obtained after 24 h was maintained or increased over time.

Poor Vitamin C Status Late in Pregnancy Is Associated with Increased Risk of Complications in Type 1 Diabetic Women: A Cross-Sectional Study

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Bente Juhl

2017-02-01

Full Text Available Vitamin C (vitC is essential for normal pregnancy and fetal development and poor vitC status has been related to complications of pregnancy. We have previously shown lower vitC status in diabetic women throughout pregnancy compared to that of non-diabetic controls. Here, we evaluate the relationship between vitC status late in diabetic pregnancy in relation to fetal outcome, complications of pregnancy, diabetic characteristics, and glycemic control based on data of 47 women from the same cohort. We found a significant relationship between the maternal vitC level > or ≤ the 50% percentile of 26.6 μmol/L, respectively, and the umbilical cord blood vitC level (mean (SD: 101.0 μmol/L (16.6 versus 78.5 μmol/L (27.8, p = 0.02; n = 12/16, while no relation to birth weight or Apgar score was observed. Diabetic women with complications of pregnancy had significantly lower vitC levels compared to the women without complications (mean (SD: 24.2 μmol/L (10.6 vs. 34.6 μmol/L (14.4, p = 0.01; n = 19 and 28, respectively and the subgroup of women (about 28% characterized by hypovitaminosis C (<23 μmol/L had an increased relative risk of complications of pregnancy that was 2.4 fold higher than the one found in the group of women with a vitC status above this level (p = 0.02, 95% confidence interval 1.2–4.4. No correlation between diabetic characteristics of the pregnant women and vitC status was observed, while a negative association of maternal vitC with HbA1c at delivery was found at regression analysis (r = −0.39, p < 0.01, n = 46. In conclusion, our results may suggest that hypovitaminosis C in diabetic women is associated with increased risk of complications of pregnancy.

Establishment of 25-OH-vitamin D reference interval by radioimmunoassay and its clinical significance

International Nuclear Information System (INIS)

Chen Jianbo; Huang Xianzhong; Hu Chaohui; Zu Yuli; Zhu Qingyi

2009-01-01

Objective: To establish reference interval of 25-OH-vitamin D (25-OH-Vit D) by radioimmunoassay and provide suggestion for clinical applications. Method: Collecting 204 healthy persons specimens, and validating and establishing reference interval of 25-OH-Vit D by treatment of outlying observations, judgement of data distributions and analysis of test results. Results: The reference interval of 25-OH-Vit D established in our laboratory is 16.0-39.7 ng/ml for adolescents and 11.7-40.0 ng/ml for adults. Conclusions: The levels of 25-OH-Vit D in humans depend on their age, sex and life style. Some people's vitamin D intake is not enough. Doctors should pay attention to the sufficient 25-OH-Vit D needed when evaluate vitamin D intake. (authors)

Vitamin C deficiency reduces muscarinic receptor coronary artery vasoconstriction and plasma tetrahydrobiopterin concentration in guinea pigs

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Skovsted, Gry Freja; Tveden-Nyborg, Pernille; Lindblad, Maiken Marie

2017-01-01

Vitamin C (vitC) deficiency is associated with increased cardiovascular disease risk, but its specific interplay with arteriolar function is unclear. This study investigates the effect of vitC deficiency in guinea pigs on plasma biopterin status and the vasomotor responses in coronary arteries...... exposed to vasoconstrictor/-dilator agents. Dunkin Hartley female guinea pigs (n = 32) were randomized to high (1500 mg/kg diet) or low (0 to 50 mg/kg diet) vitC for 10-12 weeks. At euthanasia, coronary artery segments were dissected and mounted in a wire-myograph. Vasomotor responses to potassium......-1 were unaffected by vitC status. The study shows that vitC deficiency decreases tetrahydrobiopterin concentrations and muscarinic receptor mediated contraction in coronary arteries. This attenuated vasoconstrictor response may be linked to altered production of vasoactive arachidonic acid...

L-ascorbate attenuates methamphetamine neurotoxicity through enhancing the induction of endogenous heme oxygenase-1.

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Huang, Ya-Ni; Wang, Jiz-Yuh; Lee, Ching-Tien; Lin, Chih-Hung; Lai, Chien-Cheng; Wang, Jia-Yi

2012-12-01

Methamphetamine (METH) is a drug of abuse which causes neurotoxicity and increased risk of developing neurodegenerative diseases. We previously found that METH induces heme oxygenase (HO)-1 expression in neurons and glial cells, and this offers partial protection against METH toxicity. In this study, we investigated the effects of l-ascorbate (vitamin C, Vit. C) on METH toxicity and HO-1 expression in neuronal/glial cocultures. Cell viability and damage were evaluated by 3-(4,5-dimethylthianol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) reduction and lactate dehydrogenase (LDH) release, respectively. Neuronal and glial localization of HO-1 were identified by double immunofluorescence staining. Reactive oxygen species (ROS) production was measured using the fluorochrome 2',7'-dichlorofluorescin diacetate. HO-1 mRNA and protein expression were examined by RT-qPCR and Western blotting, respectively. Results show that Vit. C induced HO-1 mRNA and protein expressions in time- and concentration-dependent manners. Inhibition of p38 mitogen-activated protein kinase (MAPK) but not extracellular signal-regulated kinase (ERK) significantly blocked induction of HO-1 by Vit. C. HO-1 mRNA and protein expressions were significantly elevated by a combination of Vit. C and METH, compared to either Vit. C or METH alone. Pretreatment with Vit. C enhanced METH-induced HO-1 expression and attenuated METH-induced ROS production and neurotoxicity. Pharmacological inhibition of HO activity abolished suppressive effects of Vit. C on METH-induced ROS production and attenuated neurotoxicity. We conclude that induction of HO-1 expression contributes to the attenuation of METH-induced ROS production and neurotoxicity by Vit. C. We suggest that HO-1 induction by Vit. C may serve as a strategy to alleviate METH neurotoxicity. Copyright © 2012 Elsevier Inc. All rights reserved.

Phasing out of the Universal Mega Dose of Vitamin-A Prophylaxis to Avoid Toxicity

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Sudip Bhattacharya

2017-01-01

Full Text Available Childhood blindness due to corneal ulceration was prevalent among poor Indian children. To tackle this situation, the National Institute of Nutrition (NIN, Hyderabad, India, Vitamin-A (Vit-A prophylaxis programme was launched nationally in 1970 after field testing. Research of Indian Council for Medical Research (ICMR documented that prevalence of Vit-A deficiency signs such as Bitot’s spot decreased among children, over a period of time. However, this decrease cannot be ascertained is due to mass Vit-A prophylaxis programme. This is because coverage was low and patchy. Improved nutrition status, wider vaccination coverage, increased rate in breast feeding and improvement of healthcare services played a crucial role. Rather many studies revealed that (mass prophylaxis to the child who is having adequate Vit-A level it may be harmful to certain group of children as a result of acute toxic symptoms. High dose of Vit-A is capable of loss of bone density-hence retarded growth may be observed in susceptible individuals. To tackle this issue food based approach should be promoted (which includes breast feeding along with timely measles vaccination. The children who have signs of Vit-A deficiency (e.g. night blindness, xeropthalmia, Bitot’s spot or post measles children should receive Vit-A in age specific daily doses for two weeks along with Vit-A rich food, like green leafy vegetables, red palm oil, liver etc. Public spirited citizens, together with scientific community in India, should discourage this “one size fit to all” approach. It will not only avoid the ill effects of high dose of Vit-A but also it will help us optimal utilization of health resources in a resource poor country like India.

Clinical effectiveness of hymenoptera venom immunotherapy: a prospective observational multicenter study of the European academy of allergology and clinical immunology interest group on insect venom hypersensitivity.

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Ruëff, Franziska; Przybilla, Bernhard; Biló, Maria Beatrice; Müller, Ulrich; Scheipl, Fabian; Seitz, Michael J; Aberer, Werner; Bodzenta-Lukaszyk, Anna; Bonifazi, Floriano; Campi, Paolo; Darsow, Ulf; Haeberli, Gabrielle; Hawranek, Thomas; Küchenhoff, Helmut; Lang, Roland; Quercia, Oliviero; Reider, Norbert; Schmid-Grendelmeier, Peter; Severino, Maurizio; Sturm, Gunter Johannes; Treudler, Regina; Wüthrich, Brunello

2013-01-01

Treatment failure during venom immunotherapy (VIT) may be associated with a variety of risk factors. Our aim was to evaluate the association of baseline serum tryptase concentration (BTC) and of other parameters with the frequency of VIT failure during the maintenance phase. In this observational prospective multicenter study, we followed 357 patients with established honey bee or vespid venom allergy after the maintenance dose of VIT had been reached. In all patients, VIT effectiveness was either verified by sting challenge (n = 154) or patient self-reporting of the outcome of a field sting (n = 203). Data were collected on BTC, age, gender, preventive use of anti-allergic drugs (oral antihistamines and/or corticosteroids) right after a field sting, venom dose, antihypertensive medication, type of venom, side effects during VIT, severity of index sting reaction preceding VIT, and duration of VIT. Relative rates were calculated with generalized additive models. 22 patients (6.2%) developed generalized symptoms during sting challenge or after a field sting. A strong association between the frequency of VIT failure and BTC could be excluded. Due to wide confidence bands, however, weaker effects (odds ratios <3) of BTC were still possible, and were also suggested by a selective analysis of patients who had a sting challenge. The most important factor associated with VIT failure was a honey bee venom allergy. Preventive use of anti-allergic drugs may be associated with a higher protection rate. It is unlikely that an elevated BTC has a strong negative effect on the rate of treatment failures. The magnitude of the latter, however, may depend on the method of effectiveness assessment. Failure rate is higher in patients suffering from bee venom allergy.

Clinical effectiveness of hymenoptera venom immunotherapy: a prospective observational multicenter study of the European academy of allergology and clinical immunology interest group on insect venom hypersensitivity.

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Franziska Ruëff

Full Text Available BACKGROUND: Treatment failure during venom immunotherapy (VIT may be associated with a variety of risk factors. OBJECTIVE: Our aim was to evaluate the association of baseline serum tryptase concentration (BTC and of other parameters with the frequency of VIT failure during the maintenance phase. METHODS: In this observational prospective multicenter study, we followed 357 patients with established honey bee or vespid venom allergy after the maintenance dose of VIT had been reached. In all patients, VIT effectiveness was either verified by sting challenge (n = 154 or patient self-reporting of the outcome of a field sting (n = 203. Data were collected on BTC, age, gender, preventive use of anti-allergic drugs (oral antihistamines and/or corticosteroids right after a field sting, venom dose, antihypertensive medication, type of venom, side effects during VIT, severity of index sting reaction preceding VIT, and duration of VIT. Relative rates were calculated with generalized additive models. RESULTS: 22 patients (6.2% developed generalized symptoms during sting challenge or after a field sting. A strong association between the frequency of VIT failure and BTC could be excluded. Due to wide confidence bands, however, weaker effects (odds ratios <3 of BTC were still possible, and were also suggested by a selective analysis of patients who had a sting challenge. The most important factor associated with VIT failure was a honey bee venom allergy. Preventive use of anti-allergic drugs may be associated with a higher protection rate. INTERPRETATION: It is unlikely that an elevated BTC has a strong negative effect on the rate of treatment failures. The magnitude of the latter, however, may depend on the method of effectiveness assessment. Failure rate is higher in patients suffering from bee venom allergy.

Feeding Vitamin C during Neonatal and Juvenile Growth Improves Learning and Memory of Rats.

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Hosseini, Mahmoud; Beheshti, Farimah; Sohrabi, Farzaneh; Vafaee, Farzaneh; Shafei, Mohammad Naser; Reza Sadeghnia, Hamid

2018-09-03

We investigated the effects of feeding vitamin C (Vit C) during neonatal and juvenile growth on learning and memory of rats. Rats after delivery were randomly divided into four groups and treated. Group 1, control group, received normal drinking water. Groups 2-4 received Vit C 10, 100, and 500 mg/kg, respectively, from the first day. After 8 weeks, 10 male offspring of each group were randomly selected and tested in the Morris water maze (MWM) and passive avoidance (PA) tests. Finally, the brains were removed for biochemical measurement. In MWM, 10-500 mg/kg Vit C reduced the latency and traveled distance and increased time spent in the target quadrant. In PA, 10 and 100 mg/kg of Vit C increased the latency; 10-500 mg/kg of Vit C decreased the malondialdehyde (MDA) in the brain tissues and increased thiol and catalase (CAT) activity compared to the control group. We showed that feeding rats Vit C during neonatal and juvenile growth has positive effects on learning and memory.

A systematic review of the clinical effectiveness and cost-effectiveness of Pharmalgen® for the treatment of bee and wasp venom allergy.

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Hockenhull, J; Elremeli, M; Cherry, M G; Mahon, J; Lai, M; Darroch, J; Oyee, J; Boland, A; Dickson, R; Dundar, Y; Boyle, R

2012-01-01

Each year in the UK, there are between two and nine deaths from anaphylaxis caused by bee and wasp venom. Anaphylactic reactions can occur rapidly following a sting and can progress to a life-threatening condition within minutes. To avoid further reactions in people with a history of anaphylaxis to bee and wasp venom, the use of desensitisation, through a process known as venom immunotherapy (VIT), has been investigated and is in use in the UK. VIT consists of subcutaneous injections of increasing amounts of purified bee and/or wasp venom extract. Pharmalgen® products (ALK Abelló) have had UK marketing authorisation for VIT (as well as diagnosis) of allergy to bee venom (using Pharmalgen Bee Venom) and wasp venom (using Pharmalgen Wasp Venom) since March 1995. This review assessed the clinical effectiveness and cost-effectiveness of Pharmalgen in providing immunotherapy to individuals with a history of type 1 [immunoglobulin E (IgE)-mediated] systemic allergic reaction to bee and wasp venom. A comprehensive search strategy using a combination of index terms (e.g. Pharmalgen) and free-text words (e.g. allerg$) was developed and used to interrogate the following electronic databases: EMBASE, MEDLINE, The Cochrane Library. Papers were included if they studied venom immunotherapy using Pharmalgen (PhVIT) in patients who had previously experienced a systemic reaction to a bee and/or a wasp sting. Comparators were any alternative treatment options available in the NHS without VIT. Included outcomes were systemic reactions, local reactions, mortality, anxiety related to the possibility of future allergic reactions, health-related quality of life (QoL) and adverse reactions (ARs) to treatment. Cost-effectiveness outcomes included cost per quality-adjusted life-years (QALYs) gained. Because of the small number of published randomised controlled trials (RCTs), no meta-analyses were conducted. A de novo economic model was developed to assess the cost-effectiveness of PhVIT

Intranasal administration of vitamin D attenuates blood-brain barrier disruption through endogenous upregulation of osteopontin and activation of CD44/P-gp glycosylation signaling after subarachnoid hemorrhage in rats.

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Enkhjargal, Budbazar; McBride, Devin W; Manaenko, Anatol; Reis, Cesar; Sakai, Yasushi; Tang, Jiping; Zhang, John H

2017-07-01

In this study, we investigated the role of vitamin D3 (VitD3) on endogenous osteopontin (OPN), a neuroprotective glycoprotein, after subarachnoid hemorrhage (SAH). The endovascular perforation SAH model in Sprague-Dawley rats was used to study the effect of intranasal VitD3 (30 ng/kg) before (Pre-SAH + VitD3) and after (Post-SAH + VitD3) subarachnoid hemorrhage. Vitamin D3 (30, 60, 120 ng/kg/day) increased more than one fold endogenous OPN expression in astrocytes and endothelial cells of rat brain. Vitamin D3 significantly decreased brain edema and Evans blue extravasation. In addition, neurobehavioral scores were significantly higher in Pre-SAH + VitD3, but partly higher in Post-SAH + VitD3, group compared with SAH group. These protective effects of vitamin D3 were completely attenuated by intracerebroventricular injection of transcription inhibitor Actinomycin D and significantly inhibited by small interfering ribonucleic acid (siRNA) for vitamin D receptor and OPN in Pre-SAH + VitD3 rats. OPN expression was significantly higher in Pre-SAH + VitD3 rats, specifically A and C, but not B, isomers were upregulated in the astrocytes, leading to CD44 splicing, and P-gp glycosylation in brain endothelial cells. The results show that intranasal vitamin D3 attenuates blood-brain barrier (BBB) disruption through endogenous upregulation of OPN and subsequent CD44 and P-gp glycosylation signals in brain endothelial cells. Furthermore, this study identifies a novel strategy for the cost-effective management of subarachnoid hemorrhage.

Venom Immunotherapy in High-Risk Patients: The Advantage of the Rush Build-Up Protocol.

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Rosman, Yossi; Confino-Cohen, Ronit; Goldberg, Arnon

2017-01-01

Venom immunotherapy (VIT) is considered to be the gold standard treatment for patients with hymenoptera venom allergy. This treatment induces systemic reactions (SR) in a significant number of patients. To evaluate the outcome of VIT in patients with known risk factors for VIT-induced SR and to compare rush VIT (RVIT) and conventional VIT (CVIT). All of the patients who received VIT and had at least one of the following risk factors were included: current cardiovascular disease, uncontrolled asthma, high basal serum tryptase, current treatment with β-blockers or angiotensin-converting enzyme inhibitors, and age >70 or bee venom. Thirty-five (54.7%) patients underwent RVIT and 29 CVIT. The incidence of patients who developed SR during the build-up phase was similar for RVIT and CVIT (25.7 and 27.5%, respectively; p = 1). However, the incidence of SR per injection was significantly higher in CVIT than in RVIT (5.6 and 2.75%, respectively; p = 0.01). Most reactions (79.1%) were mild, limited to the skin. Most of the patients (92.1%) reached the full maintenance dose of 100 μg. This dose was reached by a significantly larger number of patients receiving RVIT compared to CVIT (100 and 82.7%, respectively; p = 0.01). None of the patients experienced exacerbation of their concurrent chronic disease during VIT. VIT can be performed safely and efficiently in patients with risk factors for immunotherapy. In these patients RVIT appears to be safer and more efficient than CVIT. © 2017 S. Karger AG, Basel.

Vitamin D Decreases Serum VEGF Correlating with Clinical Improvement in Vitamin D-Deficient Women with PCOS: A Randomized Placebo-Controlled Trial.

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Irani, Mohamad; Seifer, David B; Grazi, Richard V; Irani, Sara; Rosenwaks, Zev; Tal, Reshef

2017-03-28

Vascular endothelial growth factor (VEGF) has been suggested to play a role in the pathophysiology of polycystic ovary syndrome (PCOS) and may contribute to increased risk of ovarian hyperstimulation syndrome (OHSS) in affected individuals. Vitamin D (VitD) supplementation improves multiple clinical parameters in VitD-deficient women with PCOS and decreases VEGF levels in several other pathologic conditions. Unveiling the basic mechanisms underlying the beneficial effects of vitamin D on PCOS may enhance our understanding of the pathophysiology of this syndrome. It may also suggest a new treatment for PCOS that can improve it through the same mechanism as vitamin D and can be given regardless of vitamin D levels. Therefore, we aimed to explore the effect of VitD supplementation on serum VEGF levels and assess whether changes in VEGF correlate with an improvement in characteristic clinical abnormalities of PCOS. This is a randomized placebo-controlled trial conducted between October 2013 and March 2015. Sixty-eight VitD-deficient women with PCOS were recruited. Women received either 50,000 IU of oral VitD3 or placebo once weekly for 8 weeks. There was a significant decrease in serum VEGF levels (1106.4 ± 36.5 to 965.3 ± 42.7 pg·mL -1 ; p PCOS. This is a novel molecular explanation for the beneficial effects of VitD treatment. It also suggests the need to investigate a potential role of VitD treatment in reducing the incidence or severity of OHSS in VitD-deficient women with PCOS.

Oxidative stress in erythrocytes: a study on the effect of antioxidant mixtures during intermittent exposures to high altitude

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Vani, R.; Shiva Shankar Reddy, C. S.; Asha Devi, S.

2010-09-01

The aim of our study was to compare and assess the effectiveness of antioxidant mixtures on the erythrocytes (RBC) of adult male albino rats (Wister) subjected to simulated intermittent high altitudes—5,100 m (AL1) and 6,700 m (AL2)—to induce oxidative stress (OS). To achieve our objective, we pre-supplemented four sets of animals with different antioxidant mixtures [vitamin E (vit.E; 50 IU/kg BW), vitamin C (vit.C; 400 mg/kg) and l-carnitine (400 mg/kg)] in different combinations [M1 (vit.E+vit.C), M2 (vit.C+carnitine), M3 (vit.E+carnitine) and M4 (vit.C+vit.E+carnitine)] for 30 days prior to as well during exposure to intermittent hypobaric hypoxia (IHH). Membrane instability, in terms of osmotic fragility and hemolysis, decreased in RBCs of supplemented animals. There was a significant increase in the activity of glutathione peroxidase in the RBCs of supplemented animals. We confirmed OS imposed by IHH with assays relating to lipid [thiobarbituric acid reactive substances (TBARS) and lipofuscin (LF)] and protein (carbonyl, PrC) oxidation, and found a positive correlation between PrC and hemolysis, with a decrease in both upon supplementation with M3 and M4 mixtures. Fluorescence microscopic observation showed a maximum decrease in the LF content in rats administered M4 and M1 compared to those on M2 and M3 mixtures at both altitudes. We suggest that multiple antioxidant fortifications are effective in overcoming increased OS experienced by RBCs at high altitudes.

Vitamin K2 can suppress the expression of Toll-like receptor 2 (TLR2) and TLR4, and inhibit calcification of aortic intima in ApoE-/- mice as well as smooth muscle cells.

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Wang, Zhaojun; Wang, Zhongqun; Zhu, Jie; Long, Xinguang; Yan, Jinchuan

2018-02-01

Background and objectives Vascular calcification is a common complication in atherosclerosis. Accumulating evidence showed that Toll-like receptors (TLRs) mediate pro-inflammatory and atherosclerosis. Recent studies demonstrated that vascular calcification is one of the detrimental effects of vitamin K (Vit K) antagonists. However, the effects of Vit K on the expression of TLR2 and 4 and intimal calcification in artery remained unidentified. Methods and results Eighteen ApoE -/- mice were randomly divided into model group, Vit K-treated group, and control group. The mice of model and Vit K-treated group were fed with high-fat diet, while control group mice were fed with normal diet. Mice of Vit K-treated group were administered orally with vitamin K2 (40 mg.kg -1 .day -1 ) for 12 weeks. Twelve weeks later the aortic sections of mice were acquired and stained with hematoxylin and eosin and von Kossa, respectively. Calcium content and activity of alkaline phosphatase (ALP) at aortic tissues were measured. The expression levels of TLR2 and TLR4 in aorta sections were detected by immunohistochemisty and RT-PCR, respectively. The effects of Vit K on cellular calcification were further studied in A7r5 SMCs. Results demonstrated that high-fat diet induced typical atherosclerosis with intimal calcification in ApoE -/- mice, while in Vit K-treated group atherosclerosis and calcium deposits were not serious; Vit K2 also inhibited cellular calcification in A7r5 SMCs. Quantitative analysis showed that calcium and ALP activity at aortic tissues in the Vit K-treated mice were significantly lower than that of the model group ( P < 0.01); Compared to the control group, the expression levels of TLR2 and TLR4 in the model group were significantly higher ( P < 0.05), while in Vit K-treated group the levels of TLR2 and 4 were significantly lower than that in the model group. Furthermore, the content of calcium was positively related to the expression levels of TLR2 and TLR4

25-Hydroxyvitamin D, IL-31, and IL-33 in Children with Allergic Disease of the Airways

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Anna Bonanno

2014-01-01

Full Text Available Low vitamin D is involved in allergic asthma and rhinitis. IL-31 and IL-33 correlate with Th2-associated cytokines in allergic disease. We investigated whether low vitamin D is linked with circulating IL-31 and IL-33 in children with allergic disease of the airways. 25-Hydroxyvitamin D [25(OH Vit D], IL-31, and IL-33 plasma levels were measured in 28 controls (HC, 11 allergic rhinitis (AR patients, and 35 allergic asthma with rhinitis (AAR patients. We found significant lower levels of 25(OH Vit D in AR and in AAR than in HC. IL-31 and IL-33 plasma levels significantly increased in AAR than HC. IL-31 and IL-33 positively correlated in AR and AAR. 25(OH Vit D deficient AAR had higher levels of blood eosinophils, exacerbations, disease duration, and total IgE than patients with insufficient or sufficient 25(OH Vit D. In AAR 25(OH Vit D levels inversely correlated with total allergen sIgE score and total atopy index. IL-31 and IL-33 did not correlate with 25(OH Vit D in AR and AAR. In conclusion, low levels of 25(OH Vit D might represent a risk factor for the development of concomitant asthma and rhinitis in children with allergic disease of the airways independently of IL-31/IL-33 Th2 activity.

Desempenho vitícola de variedades autóctones italianas em condição de elevada altitude no Sul do Brasil

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Alberto Fontanella Brighenti

2014-06-01

Full Text Available O objetivo deste trabalho foi avaliar o desempenho vitícola de 12 variedades autóctones italianas cultivadas a 1.400 m de altitude, em São Joaquim, SC. As seguintes variedades foram avaliadas em três ciclos: Prosecco, Verdicchio e Vermentino (brancas; e Aglianico, Aleatico, Ancellotta, Lambrusco Grasparossa, Montepulciano, Negroamaro, Rebo, Sagrantino e Sangiovese (tintas. Utilizou-se o delineamento experimental inteiramente casualizado, com cinco repetições de oito plantas para cada variedade. Os estádios fenológicos analisados foram: brotação, floração, mudança de cor das bagas e maturidade. No momento da colheita, avaliaram-se: produtividade e produção por planta, teor de sólidos solúveis totais (ºBrix, acidez total titulável (meq L-1, pH, e teores de antocianinas e de polifenóis totais. A amplitude térmica em torno de 10°C, durante a maturação, influenciou positivamente a qualidade da uva. As variedades brancas mais bem adaptadas às condições de São Joaquim foram a Vermentino e a Verdicchio, com produtividades acima de 3,9 Mg ha-1, sólidos solúveis acima de 18°Brix e teores de polifenóis totais acima de 500 mg L-1. As variedades tintas mais bem adaptadas foram a Sangiovese, a Sagrantino e a Montepulciano, com produtividades em torno de 6 Mg ha-1, sólidos solúveis entre 19 e 21,5°Brix e teores de polifenóis totais acima de 780 mg L-1. As baixas temperaturas durante a brotação e o volume de precipitação pluvial são os aspectos climáticos mais limitantes ao cultivo das variedades avaliadas, em São Joaquim.

Protective effects of extracts of Vernonia amygdalina, Hibiscus sabdariffa and vitamin C against radiation-induced liver damage in rats

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Adaramoye, Oluwatosin; Ogungbenro, Bayo; Anyaegbu, Oluchi; Fafunso, Michael

2008-01-01

The radioprotective efficacy of methanolic extracts of leaves of Vernonia amygdalina (VA) and Hibiscus sabdariffa (HS), and vitamin C (VIT C) against gamma radiation (4 Gy) induced liver damage was studied in male Wistar albino rats. VIT C was administered at a dose of 250 mg/kg body weight, while VA and HS were administered at doses; 200, 400 and 800-mg/kg body weight, orally for 4 weeks prior to radiation and 5 weeks after irradiation. The rats were sacrificed at 24 hours and 5 weeks after irradiation. Treatment with VIT C and VA (800 mg/kg) significantly (p<0.05) decreased the gamma radiation-induced increases in serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities at 24 hours after irradiation, whereas, HS (400 mg/kg) significantly (p<0.05) decreased the serum ALT activity only. Similarly, treatment with VIT C and VA (800 mg/kg) significantly (p<0.05) decreased the serum conjugated bilirubin levels by 56% and 29%, respectively at 24 hours. Furthermore, VIT C, VA and HS significantly (p<0.05) decreased the levels of serum lipid peroxidation (LPO) and increased the hepatic superoxide dismutase (SOD) activities at 24 hours. Treatment for 5 weeks after irradiation with VIT C, VA and HS significantly (p<0.05) decreased the levels of unconjugated bilirubin, while VIT C and VA alone decreased the levels of conjugated bilirubin. Furthermore, treatment with VA (400 and 800 mg/kg) decreased the serum ALT activities by 25% and 34%, respectively, at 5 weeks after irradiation. Similarly, alkaline phosphatase and lipid peroxidation (LPO) levels were significantly (p<0.05) attenuated following treatment with VIT C and VA (400 and 800 mg/kg) at 5 weeks after irradiation. In addition, treatment with VIT C, VA (800 mg/kg) and HS (400 and 800 mg/kg) significantly (p<0.05) elevated the levels of reduced glutathione (GSH) by 61%, 56%, 41% and 44%, respectively, at 5 weeks. Similar elevation of antioxidant enzymes; SOD, glutathione-s-transferase and

Efeitos de níveis de ácido L-glutâmico e de vitamina K da dieta sobre a atividade de alfa-amilase em frangos de corte Effects of dietary levels of L-glutamic acid and vitamin K in the activity of alpha-amylase of chicks

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Flavia Escapini Fanchiotti

2005-04-01

Full Text Available Foram investigados os efeitos nutricionais de dois níveis de ácido L-glutâmico (L-Glu combinados com quatro níveis de vitamina K (Vit K sobre a atividade de alfa-amilase no quimo e pâncreas de aves de corte. Frangos de corte machos de um dia foram criados em baterias aquecidas e alimentados, à vontade, com dietas contendo todos L-aminoácidos essenciais, minerais e vitaminas (exceto Vit K até os 14 dias de idade. O experimento foi realizado em esquema fatorial, em delineamento inteiramente casualizado 2x4, com quatro repetições de oito aves cada. A dieta básica foi suplementada com 6,25 e 12,5% de L-Glu combinados com 0,02; 0,2; 20,0 e 200,0 mg de Vit K/kg de ração. Efeitos significativos de L-Glu e Vit K foram observados no quimo. A atividade específica máxima (1.005,78±245,25 UI/mg de proteína foi observada nas aves alimentadas com 6,25% de L-Glu e 20,0 mg de Vit K. Houve redução da atividade com a suplementação de 12,5% de L-Glu. No pâncreas, não houve interação entre L-Glu e Vit K, todavia, foi observado efeito de L-Glu sobre as atividades relativas, expressas em UI/g de tecido e UI/100 g de peso corporal. Os resultados sugerem que a associação entre L-Glu e Vit K interfere na atividade enzimática de alfa-amilase em aves de corte submetidas a dietas purificadas. Os resultados indicam que o nível de 12,5% de L-Glu associado aos diferentes níveis de vitamina K reduziu a atividade enzimática.The effects of two levels of L-glutamic acid (L-Glu combined with four levels of vitamin K (Vit K were studied with the objective of evaluating the activities of alpha-amylase in the chyme and pancreas of chicks. Day-old male broilers were reared in eletrically heated batteries, fed with purified amino acids diets, minerals and vitamins (except Vit K at the requirement levels, for 14 days. Feed and water were provided ad libitum. The experimental design was a factorial 2x4 with four replicates with eight chicks each The

[Tuberculosis: plasma levels of vitamin D and its relation with infection and disease].

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Esteve Palau, Erika; Sánchez Martínez, Francesca; Knobel Freud, Hernando; López Colomés, José-Luís; Diez Pérez, Adolfo

2015-02-02

Vitamin D (vitD) is involved in the phosphor-calcium metabolism and bone pathology, but also in inflammatory and infectious processes such as tuberculosis. The present study evaluates the clinical and epidemiological aspects of active tuberculosis cases and latently infected contacts in whom plasma concentrations of vitD were obtained to determine whether the deficiency of vitD is a risk factor to develop active tuberculosis, especially the more severe forms. Observational, retrospective study that included 86 tuberculosis patients and 80 contacts with latent infection in a 2-year period. When comparing active tuberculosis cases with latent infection contacts, deficiency of vitD (vitD levels <10 ng/mL, odds ratio [OR]: 2.02, 95% confidence interval [CI]: 1.04 to 3.93), male sex (OR: 1.9, 95% CI: 0.96 to 3.71) and non-white race (OR: 0.7, 95% CI: 0.34 to 1.42) were factors independently associated with the diagnosis of tuberculosis. Despite the limited number of subjects studied, there was a association between severe deficit of vitD and the presentation of tuberculosis. Copyright © 2013 Elsevier España, S.L.U. All rights reserved.

Effect of the vitamin D photosynthesis products on thermodynamic parameters of model lipid membranes

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Lisetski L. N.

2012-04-01

Full Text Available Aim. To compare effects of vitamin D (VitD, provitamin D (ProD and its photo- and thermoisomerization products on thermodynamical parameters of hydrated dipalmitoylphoshpatidylcholine (DPPC multilayers. Methods. Differential scanning calorimetry, UV spectroscopy. Results. A regular decrease was established in the melting temperature accompanied with the pronounced broadening of the appropriate peaks for DPPC multilayers doped with the sterols in the order ProD3 < < ProD3 + UV < ProD3 + UV + dark storage < VitD3. Conclusions. The destabilizing effect of VitD3 on the membrane appeared to be stronger than that of ProD3 and its photoisomerization products. This can facilitate VitD3 withdrawal from the membrane into intercellular space under its biosinthesis in vivo. A possible molecular mechanism of the phenomena observed is related to the higher conformational flexibility and anisometry of VitD3 as compared to ProD3.

The effects of vitamin E on brain derived neurotrophic factor, tissues oxidative damage and learning and memory of juvenile hypothyroid rats.

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Baghcheghi, Yousef; Beheshti, Farimah; Shafei, Mohammad Naser; Salmani, Hossein; Sadeghnia, Hamid Reza; Soukhtanloo, Mohammad; Anaeigoudari, Akbar; Hosseini, Mahmoud

2018-06-01

The effects of vitamin E (Vit E) on brain derived neurotrophic factor (BDNF) and brain tissues oxidative damage as well as on learning and memory impairments in juvenile hypothyroid rats were examined. The rats were grouped as: (1) Control; (2) Propylthiouracil (PTU); (3) PTU-Vit E and (4) Vit E. PTU was added to their drinking water (0.05%) during 6 weeks. Vit E (20 mg/kg) was daily injected (IP). Morris water maze (MWM) and passive avoidance (PA) were carried out. The animals were deeply anesthetized and the brain tissues were removed for biochemical measurements. PTU increased the escape latency and traveled path in MWM (P E (P E improved BDNF, thiol, SOD and CAT while diminished MDA. The results of the present study showed that Vit E improved BDNF and prevented from brain tissues oxidative damage as well as learning and memory impairments in juvenile hypothyroid rats.

Kaposi's Sarcoma: clinical and pathological aspects in patients seen at the Hospital Universitário Cassiano Antônio Moraes - Vitória - Espírito Santo - Brazil Sarcoma de Kaposi: achados clínico-patológicos nos pacientes atendidos no Hospital Universitário Cassiano Antônio Moraes - Vitória - Espírito Santo - Brazil

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Ricardo Montibeler Tiussi

2012-04-01

Full Text Available BACKGROUND: Kaposi's sarcoma is a neoplasm of endothelial origin that is divided into four distinct types according to the clinical characteristics and the affected population: Classic (in elder men of Jewish or Mediterranean origin; Epidemic (in patients affected by AIDS; Endemic (in black African men and Iatrogenic (in patients under immunosuppressive regimens. Human herpesvirus 8 infection is essential but not sufficient for the sarcoma development. OBJECTIVE: To describe the epidemiological, clinical and histopathological aspects of patients with KS seen at the Dermatology Clinic -Cassiano Antônio Moraes University Hospital - Federal University of Espirito Santo, Vitória - ES. METHODS: A descriptive and retrospective study based on clinical charts of patients with KS seen at the Dermatology Clinic from 1986 to 2009. RESULTS: The majority of the 15 cases were male patients (93,3% and white (60%. Epidemic Kaposi's sarcoma occurred in 80%, and the Classic form in 20%, with no cases in the Endemic or Iatrogenic groups. All the histopatho logical exams of the cutaneous lesions were reviewed and a proliferation of fusiform cells, extravasated erythrocytes and vascular rifts among the largest vessels, assuming the "vessels in vessels" typical aspect, were seen. CONCLUSION: The number of cases of Kaposi's Sarcoma was linear throughout the years of the study, especially of the epidemic form, although the incidence and prevalence of AIDS increased in the state of Espírito Santo. Therefore, if we consider the relation between KS and AIDS, a decreasing line of Kaposi's sarcoma could be seen, especially after the introduction of HAART.FUNDAMENTOS: O Sarcoma de Kaposi é neoplasia de origem endotelial, dividida em quatro formas clínicas: clássica (homens idosos de origem judaica e mediterrânea, epidêmica (associada ao HIV, endêmica (negros africanos e iatrogênica (relacionada à imunossupressão. A infecção pelo herpes vírus humano tipo 8 (HHV

Are the optimum levels of the catalase and vitamin E in rooster semen extender after freezing-thawing influenced by sperm concentration?

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Moghbeli, Morteza; Kohram, Hamid; Zare-Shahaneh, Ahmad; Zhandi, Mahdi; Sharafi, Mohsen; Nabi, Mohammad Mehdi; Zahedi, Vahid; Sharideh, Hossein

2016-06-01

To date, there has no report to evaluate the interaction effects of antioxidant and sperm concentration in rooster semen cryopreservation. This study was aimed to investigate the effects of vitamin E (VitE) and catalase (CAT) at different sperm concentrations on the rooster post-thawed sperm quality. Semen samples were collected twice a week from ten roosters (ROS 308) and diluted according to experimental treatments. The treatments consist of different sperm concentrations (200, 400 and 600 × 106 sperm/mL) with supplementation VitE (5 μg/mL; VitE200, VitE400, and VitE600, respectively) or CAT (100 IU/mL; CAT200, CAT400, CAT600, respectively) and without antioxidants [Control (Con); Con200, Con400, Con600, respectively]. After thawing, motion characteristics were assessed using a CASA system. Plasma membrane integrity and malondialdehyde (MDA) level were evaluated with Hypoosmotic swelling test (HOST) and Thiobarbituric acid (TBA), respectively. The higher percentage of total motility, progressive motility, viability and membrane integrity were obtained in VitE400 (81.16 ± 1.21, 18.44 ± 1.19, 85.47 ± 1.07, 86.91 ± 1.16, respectively) and CAT400 (79.38 ± 1.21, 17.19 ± 1.19, 83.42 ± 1.07, 85.73 ± 1.16, respectively) compared to control groups. Moreover, the lowest percentage of MDA was measured in VitE400, VitE600 and CAT400 rather than other groups (1.489, 1.500, 1.510 ± 0.06, respectively). In conclusion, the results of the present study demonstrate that VitE (5 μg/mL) and CAT (100 IU/mL) independently at sperm concentration, 400 million sperm/mL could beneficial effect for preservation of rooster semen during cryopreservation. Copyright © 2016 Elsevier Inc. All rights reserved.

Teor de clorofila e índice SPAD no abacaxizeiro cv. vitória em função da adubação nitrogenada

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Francisco de Assis Pereira Leonardo

2013-06-01

Full Text Available Tendo em vista a demanda do mercado por novas variedades e a necessidade de melhoria no manejo nutricional do abacaxizeiro, realizou-se este experimento com objetivo de avaliar o teor de clorofila e o índice SPAD no abacaxizeiro 'Vitória' em função da adubação nitrogenada com ureia e cama de frango. Para o efeito, foi instalado um experimento no delineamento em blocos casualizados, com quatro repetições. Os tratamentos consistiram em cinco doses de N, em duas fontes, orgânica (cama de frango e mineral (ureia, totalizando nove combinações geradas através da matriz Composto Central de Box: T1, 44 e 2,91; T2, 152 e 0; T3, 0 e 10; T4, 44 e 17,1; T5, 260 e 2,91; T6, 152 e 10; T7, 152 e 20; T8, 304 e 10; e T9, 260 e 17,1 g planta-1. Foram avaliados o índice SPAD, o teor de clorofila na folha e o teor de N na folha D. Para o teor relativo de clorofila (SPAD, estimou-se o máximo valor com as doses de 16,37 g planta-1 de ureia e 200,69 g planta-1 de cama de frango. Para o teor de clorofila na folha D, obteve-se o valor máximo estimado com 19,4 g planta-1 de ureia. O índice SPAD possui correlação positiva com o teor de clorofila e com o teor de N na folha.

Aging Adults and Seasonal Influenza: Does the Vitamin D Status (HArm the Body?

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Pierre Olivier Lang

2012-01-01

Full Text Available Vitamin D (VitD, although originally described as an essential hormone for bone and mineral homeostasis, appears to have an active role in regulating specific facets of human immunity. Indeed, VitD has been shown to have significant effects on cytokine production and lymphocyte proliferation. Evidence that VitD affects clearance of selected pathogens is supported by epidemiological and clinical data, while its coadministration with influenza vaccine in mice enhanced both mucosal and systemic antibody responses. This paper aims to examine how VitD may contribute to limiting the burden of influenza infection in the aging and aged adults, a population in which this burden remains considerable. Furthermore, we discuss how VitD status may play a role in host resistance to influenza virus and influence the immunogenicity of the influenza vaccines currently licensed for adults aged 65 years or over by its effects on innate and adaptive immunities.

Estimated Nutritive Value of Low-Price Model Lunch Sets Provided to Garment Workers in Cambodia

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Jan Makurat

2017-07-01

Full Text Available Background: The establishment of staff canteens is expected to improve the nutritional situation of Cambodian garment workers. The objective of this study is to assess the nutritive value of low-price model lunch sets provided at a garment factory in Phnom Penh, Cambodia. Methods: Exemplary lunch sets were served to female workers through a temporary canteen at a garment factory in Phnom Penh. Dish samples were collected repeatedly to examine mean serving sizes of individual ingredients. Food composition tables and NutriSurvey software were used to assess mean amounts and contributions to recommended dietary allowances (RDAs or adequate intake of energy, macronutrients, dietary fiber, vitamin C (VitC, iron, vitamin A (VitA, folate and vitamin B12 (VitB12. Results: On average, lunch sets provided roughly one third of RDA or adequate intake of energy, carbohydrates, fat and dietary fiber. Contribution to RDA of protein was high (46% RDA. The sets contained a high mean share of VitC (159% RDA, VitA (66% RDA, and folate (44% RDA, but were low in VitB12 (29% RDA and iron (20% RDA. Conclusions: Overall, lunches satisfied recommendations of caloric content and macronutrient composition. Sets on average contained a beneficial amount of VitC, VitA and folate. Adjustments are needed for a higher iron content. Alternative iron-rich foods are expected to be better suited, compared to increasing portions of costly meat/fish components. Lunch provision at Cambodian garment factories holds the potential to improve food security of workers, approximately at costs of <1 USD/person/day at large scale. Data on quantitative total dietary intake as well as physical activity among workers are needed to further optimize the concept of staff canteens.

Fruit size and quality of pineapples cv. Vitória in response to micronutrient doses and way of application and to soil covers

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Aiala Vieira Amorim

2011-10-01

Full Text Available The objective of this study was to evaluate the effects of foundation and leaf fertilization with micronutrients on fruit size and quality of pineapple cv. Vitória under the environmental conditions of the Baixo Acaraú irrigated perimeter in Northern Ceará State, Brazil, under two covers (bagana and black plastic of the sandy soil of low fertility. The experimental design was a randomized split blocks one with four levels of soil dressing and four levels of foliar fertilization, with five replications. Micronutrient soil dressing was studied as FTE-12 at doses of 0, 60, 120 and 180 kg ha-1. The four levels of foliar fertilization were: LF0 (without fertilizer, LF 1 (15 leaf fertilization, using the amount of 1158.75 g Fe ha-1, 844.65 g Mn ha-1, 391.5 g ha-1 Zn, 322.65 g ha-1 Cu and 216 g ha-1 B, LF2 (15 leaf fertilization, using twice the quantities of level LF1 and LF3 (15 leaf fertilization, using three times the amount of level LF1. At 13 months after planting the micropropagated plantlets was carried out the floral induction treatment and five months later the fruit harvest determining the following variables: fruit weight and median diameter, soluble solids content (SS and titratable acidity (TA. Both fruit weight and diameter increased with increasing doses of micronutrients applied to the soil and to the leaves, of plants grown both on bagana soil cover and plastic mulch. On the other hand fruit pulp quality was little affected by the treatments studied. There were a small increase of SS contents for plants grown on bagana soil cover and a small decrease of titratable acidity for those grown on plastic mulch, in both cases just in response to micronutrient foliar application.

O processo de trabalho do técnico em saúde bucal e suas relações com a equipe de saúde bucal na Região Metropolitana da Grande Vitória, Espírito Santo, Brasil Dental hygienist technicians' working process and their relationship with the oral health team in the Metropolitan Region of Vitória, Espírito Santo, Brazil

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Carolina Dutra Degli Esposti

2012-06-01

Full Text Available O objetivo deste estudo é analisar a compreensão dos cirurgiões-dentistas (CDs sobre o processo de trabalho e as relações da equipe de saúde com os Técnicos em Saúde Bucal (TSBs. Em cinco municípios da Região Metropolitana da Grande Vitória (RMGV, Espírito Santo (ES, Brasil, foram selecionados 18 sujeitos, a partir de uma amostra probabilística de 217 CDs que atuavam no Sistema Único de Saúde (SUS. Os sujeitos foram entrevistados a partir de um roteiro-guia semiestruturado, do qual constavam temas relacionados à organização do trabalho na saúde bucal; às atribuições e responsabilidades do TSB; à relação entre o cirurgião-dentista e o TSB; à relação com a equipe de saúde e autonomia do TSB. Os dados foram analisados a partir da Análise de Conteúdo sugerida por Bardin. Constatou-se que os CDs valorizam a importância da participação dos TSBs na reorganização do trabalho odontológico e a construção de uma relação de parceria e cooperação. Contudo, os CDs reconhecem que a relação com os TSBs é prejudicada pela desinformação sobre o processo de trabalho. Além disso, os CDs demonstram receios quanto à possibilidade de os TSBs tornarem-se dentistas práticos e tomarem seu espaço no mercado de trabalho. Também há preocupações sobre a responsabilização legal dos CDs sobre atividades desenvolvidas pelos TSBs.This study aims to assess dentists' understanding about the working process and the relationships of the health team with dental hygienist technicians (DHT. In five cities of the Metropolitan Region of Vitória, State of Espírito Santo, Southeastern Brazil, 18 subjects were selected from a probability sample of 217 dentists that worked in the National Health System. Subjects were interviewed by means of a semi-structured script, which contained topics related to work organization in oral health; roles and responsibilities of the DHT; relationship between the dentist and the DHT; DHT

Physicochemical characterization of native and modified sodium caseinate- Vitamin A complexes.

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Gupta, Chitra; Arora, Sumit; Syama, M A; Sharma, Apurva

2018-04-01

Native and modified sodium caseinate- Vitamin A complexes {Sodium caseinate- Vit A complex by stirring (NaCas-VA ST), succinylated sodium caseinate- Vit A complex by stirring (SNaCas-VA ST), reassembled sodium caseinate- Vit A complex (RNaCas-VA) and reassembled succinylated sodium caseinate- Vit A complex (RSNaCas-VA)} were prepared and characterized for their physicochemical characteristics e.g. particle size, zeta potential, turbidity analysis and tryptophan intensities which confirmed structural modification of both native (NaCas-VA ST) and modified (SNaCas-VA ST, RNaCas-VA and RSNaCas- VA) proteins upon complex formation with vitamin A. Binding of vitamin A to milk protein reduced the turbidity caused by vitamin A, however, the particle size and zeta potential of milk protein increased after complexation. Microstructure details of NaCas (spray dried) showed uniform spherical structure, however, other milk proteins and milk protein- Vit A complexes (freeze dried) showed broken glass and flaky structures. Tiny particles were observed on the surface of reassembled protein and reassembled protein- Vit A complexes. Binding of vitamin A to milk protein did not have an influence on the electrophoretic mobility and elution profile (RP-HPLC). Copyright © 2018 Elsevier Ltd. All rights reserved.

Arsenic induced toxicity in broiler chicks and its alleviation with ascorbic acid: a toxico-patho-biochemical study

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Khan, Ahrar; Sharaf, Rabia; Khan, Muhammad Zargham; Saleemi, Muhammad Kashif; Mahmood, Fazal

2013-01-01

To find out toxico-pathological effects of arsenic (As) and ameliorating effect of ascorbic acid (Vit C), broilers birds were administered 50 and 250 mg/kg arsenic and Vit C, respectively alone/in combination. As-treated birds exhibited severe signs of toxicity such as dullness, depression, increased thirst, open mouth breathing and watery diarrhea. All these signs were partially ameliorated with the treatment of Vit C. As-treated birds showed a significant decrease in serum total proteins while serum enzymes, urea and creatinine were significantly increased. Alkaline phosphatase and lactate dehydrogenase completely whereas proteins, aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea and creatinine were partial ameliorated in birds treated with As+Vit C as compared to As-treated and control birds. Pale and hemorrhagic liver and swollen kidneys were observed in As-treated birds. Histopathologically, liver exhibited congestion and cytoplasmic vacuolation while in kidneys, condensation of tubular epithelium nuclei, epithelial necrosis, increased urinary spaces, sloughing of tubules from basement membrane and cast deposition were observed in As-treated birds. Pathological lesions were partially ameliorated with the treatment of Vit C. It can be concluded that arsenic induces biochemical and histopathological alterations in broiler birds; however, these toxic effects can be partially attenuated by Vit C.

The role of vitamin E or clay in growing Japanese quail fed diets polluted by cadmium at various levels.

Science.gov (United States)

Abou-Kassem, D E; Mahrose, Kh M; Alagawany, M

2016-03-01

This study was conducted to verify whether vitamin (Vit) E or natural clay as feed additives has the potential to modulate the deleterious effects resulting from exposure to cadmium (Cd) in growing Japanese quail. 648 Japanese quail chicks (1 week old) were used to evaluate the effects of dietary Cd (0, 40, 80 and 120 mg/kg diet) and two levels of Vit E (0, 250 mg/kg diet) or two levels of natural clay (0 and 100 mg/kg diet) to study the influences of Cd, Vit E, clay or their different combinations on growth performance, carcass traits, some blood biochemical components and Cd residues in muscles and liver. Live BW and weight gain of quails were linearly decreased with increasing dietary Cd levels. Moreover, feed conversion was significantly worsened with increasing Cd level. Mortality percentage was linearly increased as dietary Cd level increased up to 120 mg/kg diet. Carcass percentage was linearly decreased as dietary Cd level increased. While, giblets percentage were linearly and quadratically differed as dietary Cd level increased. Cd caused significant changes in total plasma protein, albumin, globulin, A/G ratio, creatinine, urea-N and uric acid concentrations as well as ALT, AST and ALP activities. Increasing dietary Cd level was associated with its increase in the muscles and liver. Dietary supplementation with 250 mg of Vit E/kg diet or 100 mg clay/kg improved live BW, BW gain and feed conversion when compared with the un-supplemented diet. Quails fed diet contained 250 mg Vit E/kg and those fed 100 mg clay/kg had the highest percentages of carcass and dressing than those fed the un-supplemented diet. Blood plasma biochemical components studied were better when birds received 250 mg of Vit E/kg diet and those received 100 mg clay/kg. Cd residues in the muscles and liver were significantly less in the birds had 250 mg of Vit E/kg or those received 100 mg clay/kg diet than those un-supplemented with Vit E. Growth performance traits and blood plasma

Effect of sperm concentration on characteristics and fertilization capacity of rooster sperm frozen in the presence of the antioxidants catalase and vitamin E.

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Moghbeli, Morteza; Kohram, Hamid; Zare-Shahaneh, Ahmad; Zhandi, Mahdi; Sharideh, Hossein; Sharafi, Mohsen

2016-10-01

The objective of this study conducted was to determine the influence of different levels of sperm concentration, including catalase (CAT) and vitamin E (VitE) in rooster semen extender on postthawed quality and fertility of rooster semen. Semen was collected twice a week from six roosters (Arian) and diluted according to experimental treatments consisting of sperm suspensions containing different sperm concentrations (200, 400, and 600 × 106 sperm/mL) without antioxidant supplementation as control (Con) groups (Con200, Con400, and Con600, respectively), sperm suspensions containing different sperm concentrations (200, 400, and 600 × 106 sperm/mL) supplemented with 5-μg/mL VitE (VitE200, VitE400, and VitE600, respectively) and different sperm concentrations (200, 400, and 600 × 106 sperm/mL) supplementation with 100 IU/mL CAT (CAT200, CAT400, and CAT600, respectively). After thawing; sperm motility, membrane integrity, and mitochondrial function were assessed. Fertility and hatchability rates were determined by using 100 artificially inseminated hens. The percentage of total motility (TM) and activity of mitochondria decreased (P rooster extender with different level sperm concentrations had no effect (P > 0.05) on fertility and hatchability rates. In conclusion, although adding VitE and CAT in extender with different levels of sperm concentration improved postthawed quality of rooster semen, but adding VitE and CAT in the extender have no effect on fertility rate. Copyright © 2016 Elsevier Inc. All rights reserved.

Maternal vitamin D supplementation during pregnancy prevents vitamin D deficiency in the newborn: an open-label randomized controlled trial.

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Rodda, C P; Benson, J E; Vincent, A J; Whitehead, C L; Polykov, A; Vollenhoven, B

2015-09-01

To determine whether maternal vitamin D supplementation, in the vitamin D deficient mother, prevents neonatal vitamin D deficiency. Open-label randomized controlled trial. Metropolitan Melbourne, Australia, tertiary hospital routine antenatal outpatient clinic. Seventy-eight women with singleton pregnancies with vitamin D deficiency/insufficiency (serum 25-OH Vit D l) at their first antenatal appointment at 12-16-week gestation were recruited. Participants were randomized to vitamin D supplementation (2000-4000 IU cholecalciferol) orally daily until delivery or no supplementation. The primary outcome was neonatal serum 25-OH vit D concentration at delivery. The secondary outcome was maternal serum 25-OH vit D concentration at delivery. Baseline mean maternal serum 25-OH vit D concentrations were similar (P = 0·9) between treatment (32 nmol/l, 95% confidence interval 26-39 nmol/l) and control groups (33 nmol/l, 95% CI 26-39 nmol/l). Umbilical cord serum 25-OH vit D concentrations at delivery were higher (P l, 95% CI; 70-91 nmol/l) compared with neonates of control group mothers (42 nmol/l, 95% CI; 34-50 nmol/l) with a strongly positive correlation between maternal serum 25-OH Vit D and umbilical cord serum 25-OH vit D concentrations at delivery (Spearman rank correlation coefficient 0·88; P l, 95% CI; 62-81 nmol/l) compared with the control group (36 nmol/l, 95% CI; 29-42 nmol/l). Vitamin D supplementation of vitamin D deficient pregnant women prevents neonatal vitamin D deficiency. © 2015 John Wiley & Sons Ltd.

La expresión y actividad catalítica diferencial de GDP-manosa pirofosforilasa influye en la producción de vitamina C en Myrciaria dubia “camu-camu”

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Juan Castro Gómez

2013-12-01

Full Text Available Myrciaria dubia muestra una amplia variación en la producción de vitamina C (vit.C. Para entender las bases moleculares de esta variación, se determinó la relación del nivel de expresión genética y actividad de GDP-Manosa pirofosforilasa (GMP con la variación del contenido de vit.C en M. dubia. Las muestras fueron obtenidas de una colección de germoplasma. La vit.C se cuantificó por HPLC, la actividad de GMP se midió por espectrofotometría. El ARN se purificó, el ADNc se sintetizó, amplificó, clonó y secuenció con técnicas estándares. La expresión del gen se midió mediante PCR en tiempo real. Las diferencias en el contenido de vit.C de las hojas, pulpa y cáscara dependieron de la expresión y actividad de GMP. Esta relación también se observó entre plantas que producen frutos con bajo y alto contenido de vit.C. En conclusión, Las hojas, pulpa y cáscara de M. dubia muestran diferencias significativas en el contenido de vit.C, el cual se atribuye a la expresión y actividad catalítica diferencial de la GMP. Asimismo, las plantas que producen frutos con diferencias marcadas en el contenido de vit. C en sus pulpas, también dependen del nivel de expresión y actividad catalítica de esta enzima.

La expresión y actividad catalítica diferencial de GDP-manosa pirofosforilasa influye en la producción de vitamina C en Myrciaria dubia “camu-camu”

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Juan Castro Gómez

2013-12-01

Full Text Available Myrciaria dubia muestra una amplia variación en la producción de vitamina C (vit.C. Para entender las bases moleculares de esta variación, se determinó la relación del nivel de expresión genética y actividad de GDP-Manosa pirofosforilasa (GMP con la variación del contenido de vit.C en M. dubia. Las muestras fueron obtenidas de una colección de germoplasma. La vit.C se cuantificó por HPLC, la actividad de GMP se midió por  espectrofotometría. El ARN se purificó, el ADNc se sintetizó, amplificó, clonó y secuenció con técnicas estándares. La expresión del gen se midió mediante PCR en tiempo real. Las diferencias en el contenido de vit.C de las hojas, pulpa y cáscara dependieron de la expresión y actividad de GMP. Esta relación también se observó entre plantas que producen frutos con bajo y alto contenido de vit.C. En conclusión, Las hojas, pulpa y cáscara de M. dubia muestran diferencias significativas en el contenido de vit.C, el cual se atribuye a la expresión y actividad catalítica diferencial de la GMP. Asimismo, las plantas que producen frutos con diferencias marcadas en el contenido de vit.C en sus pulpas, también dependen del nivel de expresión y actividad catalítica de esta enzima.

Effect of vitamin E and selenium nanoparticles on post-thaw variables and oxidative status of rooster semen.

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Safa, Soroush; Moghaddam, Gholamali; Jozani, Raziallah Jafari; Daghigh Kia, Hossein; Janmohammadi, Hossein

2016-11-01

This study was conducted to determine the combined effects of adding vitamin E (VitE) and selenium nanoparticle (Nano-Se) as antioxidant supplements to rooster semen extender for freezing. Semen samples were collected from 12 White Leghorn roosters and pooled. Subsequently, the samples were divided into nine equal groups using modified Beltsville extender. Extenders were supplemented with either two amounts of VitE (5 and 10μg/mL) or two amounts of Nano-Se (1% and 2%) or a combination of both VitE and Nano-Se, and no antioxidants extender (control group). Using 5μg/mL VitE and 1% of Nano-Se improved (Prooster semen. Copyright © 2016 Elsevier B.V. All rights reserved.

Safety of 100??g venom immunotherapy rush protocols in children compared to adults

OpenAIRE

Stoevesandt, Johanna; Hosp, Christine; Kerstan, Andreas; Trautmann, Axel

2017-01-01

Background There is a paucity of studies examining the safety of venom immunotherapy (VIT) in children. We aimed to assess the incidence of anaphylactic side effects during rush VIT in a cohort of pediatric patients and adult controls. Methods 72 consecutive cycles of VIT-buildup in 71 children/adolescents aged 7?17?years were retrospectively evaluated and compared to an adult control group (n?=?981) with regard to baseline parameters (sex, causative venom, severity of index sting reaction, r...

Safety of 100 µg venom immunotherapy rush protocols in children compared to adults

OpenAIRE

Stoevesandt, Johanna; Hosp, Christine; Kerstan, Andreas; Trautmann, Axel

2018-01-01

Background: There is a paucity of studies examining the safety of venom immunotherapy (VIT) in children. We aimed to assess the incidence of anaphylactic side effects during rush VIT in a cohort of pediatric patients and adult controls. Methods: 72 consecutive cycles of VIT-buildup in 71 children/adolescents aged 7–17 years were retrospectively evaluated and compared to an adult control group (n = 981) with regard to baseline parameters (sex, causative venom, severity of index sting ...

Vitamin D and Bone Disease

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S. Christodoulou

2013-01-01

Full Text Available Vitamin D is important for normal development and maintenance of the skeleton. Hypovitaminosis D adversely affects calcium metabolism, osteoblastic activity, matrix ossification, bone remodeling and bone density. It is well known that Vit. D deficiency in the developing skeleton is related to rickets, while in adults is related to osteomalacia. The causes of rickets include conditions that lead to hypocalcemia and/or hypophosphatemia, either isolated or secondary to vitamin D deficiency. In osteomalacia, Vit. D deficiency leads to impairment of the mineralisation phase of bone remodeling and thus an increasing amount of the skeleton being replaced by unmineralized osteoid. The relationship between Vit. D and bone mineral density and osteoporosis are still controversial while new evidence suggests that Vit. D may play a role in other bone conditions such as osteoarthritis and stress fractures. In order to maintain a “good bone health” guidelines concerning the recommended dietary intakes should be followed and screening for Vit. D deficiency in individuals at risk for deficiency is required, followed by the appropriate action.

Influence of Vitamins on Secondary Reactive Oxygen Species Production in Sera of Patients with Resectable NSCLC

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Thierry Patrice

2016-07-01

Full Text Available Background: Singlet oxygen (1O2 oxidizes targets through the production of secondary reactive oxygen species (SOS. Cancers induce oxidative stress changing with progression, the resulting antioxidant status differing from one patient to the other. The aim of this study was to determine the oxidative status of patients with resectable Non-Small cell lung cancers (NSCLC and the potential influence of antioxidants, compared to sera from healthy donors. Materials and Methods: Serum samples from 10 women and 28 men, 19 adenocarcinomas (ADK, 15 patients N1 or M1 were submitted to a photoreaction producing 1O2. Then, samples were supplemented with vitamins (Vit C, Vit E, or glutathione (GSH. Results: Squamous cell carcinomas (SCC and metastatic SCCs induced a lower SOS rate. While Vit C increased SOS in controls as in patients with metastases, Vit E or the combination of Vit E and C strongly reduced SOS. GSH alone lightly decreased SOS in controls but had no effect in patients either alone or combined with Vit C. Conclusion: In “early” lung cancers, SOS are comparable or lower than for healthy persons. The role of Vitamins varies with gender, cancer type, and metastases. This suggests that an eventual supplementation should be performed on a per-patient basis to evidence any effect.

Concentration-Effect Relationship of Intrapritoneal Administration of 1, 25 (OH 2-Vitamin D in a Chronic Constriction Model of Neuropathic Pain

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Farinaz Nasirinezhad

2011-04-01

Full Text Available Introduction:  Results: These findings revealed the exaggerated responses in the group which received CCI. The group which was treated by 1 μg/kg of 1,25 vit D3 showed a significant reduction in pain behavior. Injection of 1,25 vit D3 did not change the response of animals to the acetone drop and von frey filament. Discussion: Our results showed that antinoceptive effect of 1,25 vit D3 in a rodent neuropathic pain model is dose dependent and this vitamin may provide new approach for treatment of chronic pain.The presence of nuclear receptors of 1,25 dihydroxy vitamin D3 (1,25 vit D3, the biologically active metabolite of vitamin D, in neurons and glial cells indicates the biological effect of this vitamin in the nervous system. The present experiment was conducted to identify the effects of different doses of 1,25 vit D3 on mechanical and cold allodynia in rodent model of neuropatinc pain. Methods: A mononeuropathy was produced by chronic constrictive injury (CCI of the sciatic nerve. 1,25 vit D3 (0.3, 0.6 ,1 μg/Kg was administered by an i.p. injection every 2 days during a month after CCI. Mechanical and cold allodynia were evaluated by Von frey filament and acetone respectively.

Vitamin C-linker-conjugated tripeptide AHK stimulates BMP-2-induced osteogenic differentiation of mouse myoblast C2C12 cells.

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Jung, Jung-Il; Park, Kyeong-Yong; Lee, Yura; Park, Mira; Kim, Jiyeon

2018-03-15

Vitamin C-linker-conjugated Ala-His-Lys tripeptide (Vit C-AHK) is a derivative of Vitamin C-conjugated tripeptides, which were originally developed as a component of a product for collagen synthesis enhancement or human dermal fibroblast growth. Here, we investigated the effect of Vit C-AHK on bone morphogenetic protein (BMP)-2-induced osteoblast differentiation in a cell culture model. Vit C-AHK enhanced proliferation of C2C12 cells and induction of BMP-2-induced alkaline phosphatase, a typical marker of osteoblast differentiation. Vit C-AHK also stimulated the phosphorylation and translocation of Smad1/5/8 to the nucleus and phosphorylation of mitogen-activated protein kinases (MAPKs) including ERK1/2 and p38. In addition, Vit C-AHK enhanced the BMP-2-induced mRNA expression of osteoblast differentiation-related genes such as ALP, BMP-2, Osteocalcin, and Runx2. Our results suggest that Vit C-AHK exerts an enhancing effect on osteoblast proliferation and differentiation through activation of Smad1/5/8 and MAPK ERK1/2 and p38 signaling and without significant cytotoxicity. These results provide important data for the development of peptide-based bone-regenerative agents and treatment of bone-related disorders. Copyright © 2018 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.

Protective Effects of Vitamin E on Methotrexate-Induced Jejunal Mucosal Damage in Rats.

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Burcu, Busra; Kanter, Mehmet; Orhon, Zeynep Nur; Yarali, Oguzhan; Karabacak, Rukiye

2016-04-01

To investigate the possible protective effects of Vitamin E (Vit E) on oxidative stress and jejunal damage in the rat intestinal mucosa after methotrexate (MTX)-induced enterotoxicity. Rats were divided into 3 groups: control, MTX, and MTX+ Vit E; each group contained 8 animals. The control group was given physiological serum in addition to sunflower oil for 3 days. The second group was given sunflower oil with intragastric tube daily, followed by MTX injection (20 mg/kg intraperitoneally). To the third group, starting 3 days before injection, Vit E was given dissolved in sunflower oil (600 mg/kg orally) in addition to MTX injection. Four days after MTX injection the anesthetized rats were sacrificed, and the tissue samples obtained from their jejunums were investigated for histological and biochemical analysis. Vit E treatment significantly decreased the elevated tissue malondialdehyde levels and increased the reduced glutathione peroxidase and superoxide dismutase activities in comparison to the MTX-treated group. MTX treatment caused severe histopathological injury including mucosal erosions, inflammatory cell infiltration, necrosis, hemorrhage, and villous congestion. Vit E treatment significantly attenuated the severity of intestinal injury caused by MTX via inhibiting induced nitric oxide synthase levels and NF-κB p65 activation. Because of its reconstructing and antioxidant effects, Vit E pretreatment may have protective effects in the intestinal tissue of MTX-treated rats.

Safety of 100 µg venom immunotherapy rush protocols in children compared to adults.

Science.gov (United States)

Stoevesandt, Johanna; Hosp, Christine; Kerstan, Andreas; Trautmann, Axel

2017-01-01

There is a paucity of studies examining the safety of venom immunotherapy (VIT) in children. We aimed to assess the incidence of anaphylactic side effects during rush VIT in a cohort of pediatric patients and adult controls. 72 consecutive cycles of VIT-buildup in 71 children/adolescents aged 7-17 years were retrospectively evaluated and compared to an adult control group (n = 981) with regard to baseline parameters (sex, causative venom, severity of index sting reaction, results of allergy testing, comorbidities) and the incidence of anaphylactic adverse reactions. Compared to adults, severe index sting-induced anaphylaxis was significantly less common in children ( P  = .001). Children were more likely to suffer from bee venom allergy ( P  bee venom-specific IgE ( P  = .013), but lower serum tryptase concentrations ( P  = .014). The overall rate of VIT-induced anaphylactic reactions was higher in children than in adults (6.9% vs 2.5%, P  = .046 by univariate analysis). In the final binary logistic regression model, however, only bee VIT ( P  = .039; odds ratio 2.25; confidence interval 1.04-4.87) and 5-day compared to 3-day buildup protocols ( P  = .011; odds ratio 2.64; confidence interval 1.25-5.57) were associated with an increased risk of treatment-induced anaphylaxis. All pediatric patients finally reached and tolerated the target maintenance dose of 100 µg. The higher anaphylactic reaction rate observed in pediatric patients may be attributed to a greater prevalence of bee venom allergy. VIT-induced anaphylaxis in children is usually mild and does not affect further updosing and maintenance of VIT.

Antioxidant ameliorating effects against H2O2-induced cytotoxicity in primary endometrial cells.

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Zal, F; Khademi, F; Taheri, R; Mostafavi-Pour, Z

2018-02-01

Oxidative stress and a disrupted antioxidant system are involved in a variety of pregnancy complications. In the present study, the role of vitamin E (Vit E) and folate as radical scavengers on the GSH homeostasis in stress oxidative induced in rat endometrial cells was investigated. Primary endometrial stromal cell cultures treated with 50 and 200 µM of H 2 O 2 and evaluated the cytoprotective effects of Vit E (5 µM) and folate (0.01 µM) in H 2 O 2 -treated cells for 24 h. Following the exposure of endometrial cells to H 2 O 2 alone and in the presence of Vit E and/or folate, cell survival, glutathione peroxidase (GPx) and glutathione reductase activities and the level of reduced glutathione (GSH) were measured. Cell adhesions comprise of cell attachment and spreading on collagen were determined. Flow cytometric analysis using annexin V was used to measure apoptosis. H 2 O 2 treatment showed a marked decrease in cell viability, GPx and GR activities and the level of GSH. Although Vit E or folate had some protective effect, combination therapy with Vit E and folate attenuated all the changes due to H 2 O 2 toxicity. An increasing number of alive cells was showed in the cells exposed to H 2 O 2 (50 µM) accompanied by co-treatment with Vit E and folic acid. The present findings indicate that co-administration of Vit E and folate before and during pregnancy may maintain a viable pregnancy and contribute to its clinical efficacy for the treatment of some idiopathic infertility.

[Knowledge and prevention management of vitamin D deficiency in primary care].

Science.gov (United States)

Senan Sanz, M R; Gilaberte Calzada, Y; Olona Tabueña, N; Magallón Botaya, R

2014-01-01

To determine the knowledge and management of vitamin D (Vit D) in primary care (PC). Observational study. A total of 69 primary care centres, with 57 in Barcelona and 12 in Huesca. All medical and nursing graduates of these centres. A specifically designed questionnaire was used to collect knowledge on the actions of Vit D (on bone metabolism, cardiovascular and immune systems), its deficiency and prevention measures. Of the total of 2100 professionals, 785 completed the survey (37.78%), of whom 79.9% were women. Their mean age was 47 years (SD: 9.7). Only 4.8% knew the overall actions of Vitamin D, although the majority (66%) knew only its effects on bone metabolism. Almost two-thirds (62.4%) were unaware that its deficiency was a common problem, and 54.9% believed that photoprotection was contraindicated. Almost three-quarters of those surveyed (73.6%) never or almost never determined Vit D levels in their patients. A minority (23%) offered advice to maintain an adequate level of Vit D, with the most frequent being sun exposure (16.8%). Pediatricians were more knowledgeable (p<.001) and offered more advice for maintaining adequate levels of Vit D in blood (p<.001). Most of the professionals were unaware of the overall actions of Vit D, or that its deficiency was common, or the factors contributing to this. The advice that was more often offered to avoid its deficiency was not the safest. Paediatricians are more aware of how to prevent Vit D deficiency. Copyright © 2013 Sociedad Española de Médicos de Atención Primaria (SEMERGEN). Publicado por Elsevier España. All rights reserved.

Does Vitamin C Deficiency Promote Fatty Liver Disease Development?

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David Højland Ipsen

2014-12-01

Full Text Available Obesity and the subsequent reprogramming of the white adipose tissue are linked to human disease-complexes including metabolic syndrome and concurrent non-alcoholic fatty liver disease (NAFLD and non-alcoholic steatohepatitis (NASH. The dietary imposed dyslipidemia promotes redox imbalance by the generation of excess levels of reactive oxygen species and induces adipocyte dysfunction and reprogramming, leading to a low grade systemic inflammation and ectopic lipid deposition, e.g., in the liver, hereby promoting a vicious circle in which dietary factors initiate a metabolic change that further exacerbates the negative consequences of an adverse life-style. Large epidemiological studies and findings from controlled in vivo animal studies have provided evidence supporting an association between poor vitamin C (VitC status and propagation of life-style associated diseases. In addition, overweight per se has been shown to result in reduced plasma VitC, and the distribution of body fat in obesity has been shown to have an inverse relationship with VitC plasma levels. Recently, a number of epidemiological studies have indicated a VitC intake below the recommended daily allowance (RDA in NAFLD-patients, suggesting an association between dietary habits, disease and VitC deficiency. In the general population, VitC deficiency (defined as a plasma concentration below 23 μM affects around 10% of adults, however, this prevalence is increased by an adverse life-style, deficiency potentially playing a broader role in disease progression in specific subgroups. This review discusses the currently available data from human surveys and experimental models in search of a putative role of VitC deficiency in the development of NAFLD and NASH.

Decreased release of histamine and sulfidoleukotrienes by human peripheral blood leukocytes after wasp venom immunotherapy is partially due to induction of IL-10 and IFN-gamma production of T cells.

Science.gov (United States)

Pierkes, M; Bellinghausen, I; Hultsch, T; Metz, G; Knop, J; Saloga, J

1999-02-01

Recent studies provide evidence that venom immunotherapy (VIT) alters the pattern of cytokine production by inducing an allergen-specific T-cell shift in cytokine expression from TH2 (IL-4, IL-5) to TH1 (IFN-gamma) cytokines and also inducing the production of IL-10. This study was carried out to analyze whether these changes in cytokine production of T cells already observed 1 week after the initiation of VIT in subjects with wasp venom allergy also influence the reactivity of effector cells, such as mast cells and basophils. All subjects included in this study had a history of severe systemic allergic reactions to wasp stings and positive skin test responses with venom and venom-specific IgE in the sera. Peripheral blood leukocytes were isolated before and after the initiation of VIT (rush therapy reaching a maintenance dose of 100 microg venom injected subcutaneously within 1 week) and preincubated with or without addition of IL-10, IFN-gamma, IL-10 + IFN-gamma, anti-IL-10, or anti-IFN-gamma. After stimulation with wasp venom, histamine and sulfidoleukotriene release were assessed by ELISA and compared with spontaneous release and total histamine content. After the induction of VIT, venom-induced absolute and relative histamine and sulfidoleukotriene release were reduced. This was at least partially due to the induction of IFN-gamma and IL-10 production, because (1) neutralization of IL-10 and IFN-gamma by mAbs partially restored the release after the initiation of VIT and (2) the addition of exogenous IFN-gamma and IL-10 caused a statistically significant diminution of the venom-induced histamine and sulfidoleukotriene release before VIT. Depletion of CD2(+) T cells also restored the releasability after VIT. These data indicate that T cells (producing IL-10 and IFN-gamma after VIT) play a key role for the inhibition of histamine and sulfidoleukotriene release of effector cells.

PRX1 knockdown potentiates vitamin K3 toxicity in cancer cells: a potential new therapeutic perspective for an old drug.

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He, Tiantian; Hatem, Elie; Vernis, Laurence; Lei, Ming; Huang, Meng-Er

2015-12-21

Many promising anticancer molecules are abandoned during the course from bench to bedside due to lack of clear-cut efficiency and/or severe side effects. Vitamin K3 (vitK3) is a synthetic naphthoquinone exhibiting significant in vitro and in vivo anticancer activity against multiple human cancers, and has therapeutic potential when combined with other anticancer molecules. The major mechanism for the anticancer activity of vitK3 is the generation of cytotoxic reactive oxygen species (ROS). We thus reasoned that a rational redox modulation of cancer cells could enhance vitK3 anticancer efficiency. Cancer cell lines with peroxiredoxin 1 (PRX1) gene transiently or stably knocked-down and corresponding controls were exposed to vitK3 as well as a set of anticancer molecules, including vinblastine, taxol, doxorubicin, daunorubicin, actinomycin D and 5-fluorouracil. Cytotoxic effects and cell death events were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-based assay, cell clonogenic assay, measurement of mitochondrial membrane potential and annexin V/propidium iodide double staining. Global ROS accumulation and compartment-specific H2O2 generation were determined respectively by a redox-sensitive chemical probe and H2O2-sensitive sensor HyPer. Oxidation of endogenous antioxidant proteins including TRX1, TRX2 and PRX3 was monitored by redox western blot. We observed that the PRX1 knockdown in HeLa and A549 cells conferred enhanced sensitivity to vitK3, reducing substantially the necessary doses to kill cancer cells. The same conditions (combination of vitK3 and PRX1 knockdown) caused little cytotoxicity in non-cancerous cells, suggesting a cancer-cell-selective property. Increased ROS accumulation had a crucial role in vitK3-induced cell death in PRX1 knockdown cells. The use of H2O2-specific sensors HyPer revealed that vitK3 lead to immediate accumulation of H2O2 in the cytosol, nucleus, and mitochondrial matrix. PRX1 silencing

Diet-related cancer and prevention using anticarcinogens

African Journals Online (AJOL)

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B and C infections in the causation of liver cancer. (Turner ..... Provitamins and vitamins (β- carotene, Vit.C, VitE, polyphenols ,including epigallocatechin gallate and various ..... Farombi EO, Tahnteng JG, Agboola O, Nwankwo JO, Emerole G.O.

Significant Effects of Oral Phenylbutyrate and Vitamin D3 Adjunctive Therapy in Pulmonary Tuberculosis: A Randomized Controlled Trial.

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Akhirunnesa Mily

Full Text Available Development of new tuberculosis (TB drugs and alternative treatment strategies are urgently required to control the global spread of TB. Previous results have shown that vitamin D3 (vitD3 and 4-phenyl butyrate (PBA are potent inducers of the host defense peptide LL-37 that possess anti-mycobacterial effects.To examine if oral adjunctive therapy with 5,000IU vitD3 or 2x500 mg PBA or PBA+vitD3 to standard chemotherapy would lead to enhanced recovery in sputum smear-positive pulmonary TB patients.Adult TB patients (n = 288 were enrolled in a randomized, double-blind, placebo-controlled trial conducted in Bangladesh. Primary endpoints included proportions of patients with a negative sputum culture at week 4 and reduction in clinical symptoms at week 8. Clinical assessments and sputum smear microscopy were performed weekly up to week 4, fortnightly up to week 12 and at week 24; TB culture was performed at week 0, 4 and 8; concentrations of LL-37 in cells, 25-hydroxyvitamin D3 (25(OHD3 in plasma and ex vivo bactericidal function of monocyte-derived macrophages (MDM were determined at week 0, 4, 8, 12 and additionally at week 24 for plasma 25(OHD3.At week 4, 71% (46/65 of the patients in the PBA+vitD3-group (p = 0.001 and 61.3% (38/62 in the vitD3-group (p = 0.032 were culture negative compared to 42.2% (27/64 in the placebo-group. The odds of sputum culture being negative at week 4 was 3.42 times higher in the PBA+vitD3-group (p = 0.001 and 2.2 times higher in vitD3-group (p = 0.032 compared to placebo. The concentration of LL-37 in MDM was significantly higher in the PBA-group compared to placebo at week 12 (p = 0.034. Decline in intracellular Mtb growth in MDM was earlier in the PBA-group compared to placebo (log rank 11.38, p = 0.01.Adjunct therapy with PBA+vitD3 or vitD3 or PBA to standard short-course therapy demonstrated beneficial effects towards clinical recovery and holds potential for host-directed-therapy in the treatment of TB

Administración oral de preparado parenteral de vitamina K en anticoagulación excesiva por warfarina

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Yoleima Lozada

2012-04-01

Full Text Available La warfarina es frecuentemente usada en la terapia anticoagulante actual, su acción debe ser monitorizada usando el tiempo de protrombina expresado como International Normalized Ratio (INR; cuando se excede el rango de seguridad se puede administrar vitamina K (Vit-K, preferentemente por vía oral. Dicha presentación no está disponible en Venezuela. Se realizó un ensayo clínico, doble ciego, donde a 20 pacientes, edad 18-60 años, sin sangrado e INR inicial de 6 a 10 inclusive; les fue suspendida la warfarina e inmediatamente agrupados al azar a recibir dosis única de Vit-K (oral 1.25mg de Vit-K fraccionada de una presentación parenteral o placebo. El punto final primario, INR < 3.5 a las 24 horas de administrar la dosis, se alcanzó en 70% de los pacientes en Vit-K y 20% en placebo. La reducción absoluta del riesgo y su intervalo de confianza de 95%: RAR (IC95% = 50% (14.4 a 85.6 ρ = 0.028; NNT (IC95% = 2(1.3 a 6.9; no se registraron eventos adversos, ni INR < 2 luego de 24 horas de tratamiento. Los resultados obtenidos son consistentes con estudios donde se administró Vit-K en preparación específica para vía oral. Así la Vit-K en presentación parenteral, administrada por vía oral, es más efectiva y segura que simplemente detener la administración de warfarina para revertir la excesiva anticoagulación, en donde no exista presentación específica oral de Vit-K o ésta sea muy costosa.

Effect of rosuvastatin monotherapy and in combination with fenofibrate or omega-3 fatty acids on serum vitamin D levels.

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Makariou, Stefania E; Liberopoulos, Evangelos N; Agouridis, Aris P; Challa, Anna; Elisaf, Moses

2012-12-01

Low levels of 25(OH) vitamin D [25(OH)VitD] have been recognized as a new cardiovascular disease (CVD) risk factor. Statins seem to increase 25(OH)VitD concentration. To investigate whether combined treatment with the usual dose of rosuvastatin plus fenofibrate or omega-3 fatty acids would increase 25(OH)VitD levels compared with the high-dose rosuvastatin monotherapy in participants with mixed dislipidemia. We randomly allocated 60 patients with mixed dyslipidemia (low-density lipoprotein cholesterol: >160 mg/dL plus triglycerides: >200 mg/dL) to receive rosuvastatin 40 mg (n = 22), rosuvastatin 10 mg plus fenofibrate 200 mg (n = 21), or rosuvastatin 10 mg plus omega-3 fatty acids 2 g (n = 17) daily for 3 months. Our primary end point was changes in the levels of serum 25(OH)VitD. Rosuvastatin monotherapy was associated with a 53% increase in 25(OH)VitD (from 14.6 [1.0-38.0] to 17.8 [5.3-49.6] ng/mL; P = .000). Rosuvastatin plus micronized fenofibrate and rosuvastatin plus omega-3 fatty acids were associated with increases of 64% (from 14.1 [1.0-48.0] to 18.4 [6.7-52.4] ng/mL, P = .001) and 61% (from 10.4 [6.6-38.4] to 14.0 [9.6-37.6] ng/mL, P = .04), respectively. The changes in 25(OH)VitD after treatment were comparable in the 3 groups. High-dose rosuvastatin monotherapy and the usual dose of rosuvastatin plus fenofibrate or omega-3 fatty acids are associated with significant and similar increases in the 25(OH)VitD levels. This increase may be relevant in terms of CVD risk prevention.

Investigation of the binding affinity in vitamin B12-Bovine serum albumin system using various spectroscopic methods

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Makarska-Bialokoz, Magdalena

2017-09-01

The binding affinity between vitamin B12 (VitB12) and bovine serum albumin (BSA) has been investigated in aqueous solution at pH = 7.4, employing UV-vis absorption and steady-state, synchronous and three-dimensional fluorescence spectra techniques. Representative effects noted for BSA intrinsic fluorescence resulting from the interactions with VitB12 confirm the formation of π-π stacked non-covalent and non-fluorescent complexes in the system VitB12-BSA. All the determined parameters, the binding, fluorescence quenching and bimolecular quenching rate constants (of the order of 104 L mol- 1, 103 L mol- 1 and 1011 L mol- 1 s- 1, respectively), as well as Förster resonance energy transfer parameters validate the mechanism of static quenching. The interaction with VitB12 induces folding of the polypeptide chains around Trp residues of BSA, resulting in a more hydrophobic surrounding. Presented outcomes suggest that the addition of VitB12 can lead to the more organized BSA conformation and its more folded tertiary structure, what could influence the physiological functions of bovine serum albumin, notably in case of its overuse or abnormal metabolism.

Treatment of radiation skin lesions in various stages with different preparations of vitamin B12

International Nuclear Information System (INIS)

Ji Hui; Chen Qiang; Yang Xiaohong

1989-01-01

Since the aqueous solution of Vit. B 12 was firstly used to treat efficaciously the acute radiation injury of skin and superficial second-degree skin burn, we have successfully treated radiation skin lesions in acute, chronic and recovering stages with the cream or oil preparation of Vit. B 12 . In the period of ten years (1979-1988), altogether 418 cases of radiation or non-radiation skin injury were observed and 48 out of 49 cases with acute radiation skin ulcer treated with aqueous solution of Vit. B 12 were healed with an effective rate of 97.95% and average healing time of 12.6 days. All of 37 cases with chronic radiation skin injury treated with oil preparation of Vit. B 12 were healed or improved with an effective rate of 100% and average healing time of 21.49 days. However, in the control group, of 18 cases treated with conventional drugs only 13 were healed, the effective rate being 72.22% and healing time 63.5 days, and both of these indices were of significant difference, as compared with those treated with Vit. B 12

Assessment of vitamin K2 levels in osteoporotic patients: a case control study.

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Noori, Akram; Lashkari, Mahin; Oveisi, Sonia; Khair Khah, Mohamad Reza; Zargar, Ali

2014-07-14

The aim of this study was to measure the level of Vitamin K2 (Vit K2) in osteoporotic patients and individuals with normal bone density as controls. This case-control study was done in Outpatient Department of Rheumatology at Qazvin Boo-ali Sina Hospital in 2013. Participants were 50 patients with osteoporotic densitometry measured by DEXA (T score? -2.5) who were matched with 48 persons in control group with normal bone density (T score> -1). The level of Vit K2 in samples was measured using enzyme linked immunosorbent assay (ELISA). Data were analyzed by Mann-Whitney U test and Chi-square test. The level of Vit K2 in patients with osteoporosis was not significantly different from the control group (Median: 75.95 vs. 71.35 nmol/L, respectively; P-value: 0.709). The authors determined cut-offs 75 percentile of vitamin K2 in all participants that was 85 nmol/L and percentages of persons in two groups were similar. Although Vit K2 level in patients with osteoporosis was not significantly different from the control group, further studies are necessary to confirm the association of osteoporosis and Vit K2.

HAEMATOLOGICAL PROFILE FOLLOWING IMMUNOMODULATION DURING LATE GESTATION IN BUFFALOES (BUBALIS BUBALUS

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Z.I, Qureshi, L.A. Lodhi, H.A. Samad, N.A. Naz1 and M. Nawaz

2001-07-01

Full Text Available Thirty-two adult riverine buffaloes (Buhalis bubalus in their last trimester of pregnancy were selected and randomly divided into four groups. The buffaloes of group I served as control. Animals in group II, III and IV were treated twice (7 days apart with levamisole hydrochloride (0.5mg/kg b. w. orally, Etosol (Vit E+Se, 10ml, I/m and Bacilli Calmette Guerine (BCG (0.5 ml/animal, s/c, respectively. Blood samples were collected at weekly intervals starting day 0 untill parturition. Total erythrocytic count and packed cell volume values were higher (P<0.05 in levamisole and vit E+Se treated group of buffaloes. Haemoglobin concentration was higher (P<0.05 inVit E+Se treated group. MCV, MCH and MCHC remained unchanged among all the experimental groups. Total leukocyte count was higher (P<0.05 in levamisole treated group of buffaloes. Differential leukocyte counts (relative revealed moderate lymphocytosis in all immunomodulated groups with significantly higher counts in Vit E+Se treated buffaloes. It was inferred that levamisole and vit E-se altered some haematological values, whereas BCG did not affect the haematological parameters.

Lipid quantitation and metabolomics data from vitamin E-deficient and -sufficient zebrafish embryos from 0 to 120 hours-post-fertilization

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Melissa McDougall

2017-04-01

Full Text Available The data herein is in support of our research article by McDougall et al. (2017 [1], in which we used our zebrafish model of embryonic vitamin E (VitE deficiency to study the consequences of VitE deficiency during development. Adult 5D wild-type zebrafish (Danio rerio, fed defined diets without (E– or with VitE (E+, 500 mg RRR-α-tocopheryl acetate/kg diet, were spawned to obtain E– and E+ embryos that we evaluated using metabolomics and specific lipid analyses (each measure at 24, 48, 72, 120 hours-post-fertilization, hpf, neurobehavioral development (locomotor responses at 96 hpf, and rescue strategies. Rescues were attempted using micro-injection into the yolksac using VitE (as a phospholipid emulsion containing d6-α-tocopherol at 0 hpf or D-glucose (in saline at 24 hpf.

A Population-based survey of the prevalence of HIV, syphilis, hepatitis B and hepatitis C infections and associated risk factors among young women in Vitória, Brazil

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Miranda, Angelica Espinosa; Figueiredo, Nínive Camilo; Schmidt, Renylena; Page-Shafer, Kimberly

2017-01-01

Objective To estimate the prevalence of HIV, hepatitis B (HBV) and C (HCV) and syphilis infections and associated risk exposures in a population-based sample of young women in Vitória, Brazil. Methods From March to December 2006, a cross-sectional sample of women aged 18 to 29 years was recruited into a single stage, population-based study. Serological markers of HIV, HBV, HCV, and syphilis infections and associated risk exposures were assessed. Results Of 1,200 eligible women, 1,029 (85.8%) enrolled. Median age was 23 (interquartile range [IQR] 20, 26) years; 32.2% had ≤ 8 years of education. The survey weighted prevalence estimates were: HIV, 0.6% (95% CI), 0.1%, 1.1%); anti-HBc, 4.2% (3.0%, 5.4%); HBsAg, 0.9% (0.4%, 1.6%); anti-HCV, 0.6% (0.1%, 1.1%) and syphilis 1.2% (0.5%, 1.9%). Overall, 6.1% had at least one positive serological marker for any of the tested infection. A majority (87.9%) was sexually active, of whom 12.1% reported a previously diagnosed sexually transmitted infection (STI) and 1.4% a history of commercial sex work. Variables independently associated with any positive serological test included: older age (≥25 vs. 4× minimum wage), previously diagnosed STI, ≥ 1 sexual partner, and any illicit drug use. Conclusions These are the first population-based estimates of the prevalence of exposure to these infectious diseases and related risks in young women, a population for whom there is a scarcity of data in Brazil. PMID:18401700

Changes in gene expression caused by insect venom immunotherapy responsible for the long-term protection of insect venom-allergic patients

NARCIS (Netherlands)

Niedoszytko, Marek; Bruinenberg, Marcel; de Monchy, Jan; Weersma, Rinse K.; Wijmenga, Cisca; Jassem, Ewa; Oude Elberink, Joanne N. G.

Background: Insect venom immunotherapy (VIT) is the only causative treatment of insect venom allergy (IVA). The immunological mechanism(s) responsible for long-term protection achieved by VIT are largely unknown. A better understanding is relevant for improving the diagnosis, prediction of

Trajetórias e estratégias de mulheres: um estudo conquistense Trayectorias y estrategias de mujeres: un estudio conquistense Business women, strategies and progress: a study in vitória da conquista

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Almiralva Ferraz Gomes

2009-03-01

Full Text Available Diante de um contexto que exige posturas proativas dos gestores, esta pesquisa intentou conhecer as estratégias adotadas por mulheres empreendedoras na cidade de Vitória da Conquista, região sudoeste da Bahia. Ao longo do estudo buscou-se conhecer o seu comportamento estratégico, pois se entende que a sobrevivência dos empreendimentos, principalmente dos pequenos negócios, está relacionada à visão estratégica de seus empreendedores. A escolha de uma amostra feminina deve-se, essencialmente, ao fato de que a inserção da mulher no sistema produtivo constitui um fator privilegiado de análise das transformações que vêm ocorrendo com o feminino na modernidade. Além disso, o tema empreendedorismo feminino emerge como um assunto muito pouco estudado e carente de pesquisas não só teóricas como também empíricas. A análise dos dados coletados permitiu verificar que o comportamento estratégico predominante entre as empreendedoras, de acordo com a tipologia de Miles e Snow (1978, é o prospector.Frente a un contexto que exige posturas proactivas de los gestores, esta investigación intentó conocer las estrategias adoptadas por mujeres emprendedoras en la ciudad de Vitoria da Conquista, Región Suroeste de Bahía. A lo largo del estudio buscamos conocer su comportamiento estratégico, pues se entiende que la sobrevivencia, principalmente de los pequeños negocios, está relacionada a la visión estratégica de sus emprendedores. La elección de una muestra femenina se debe, esencialmente, al hecho de que la inclusión de la mujer en el sistema productivo constituye un factor privilegiado de análisis de las transformaciones que han ocurrido con lo femenino en la modernidad. Además de eso, el tema espíritu empresarial femenino surge como un tema muy poco estudiado y necesita investigaciones, no solo teóricas como también empíricas. El análisis de los datos colectados ha permitido verificar que el comportamiento estrat

Room-temperature ferromagnetic and photoluminescence ...

Indian Academy of Sciences (India)

Thin Films Laboratory, Center for Crystal Growth, VIT University, Vellore 632 014, India; Materials Science Group, Indira Gandhi Centre for Atomic Research, Kalpakkam 603102, India; UGC-DAE-CSR, Kalpakkam Node, Kokilamedu 603104, India; Center for Nano Research, VIT University, Vellore 603104, India ...

Gene expression analysis in predicting the effectiveness of insect venom immunotherapy

NARCIS (Netherlands)

Niedoszytko, M.; Bruinenberg, M.; de Monchy, J.; Wijmenga, C.; Platteel, M.; Jassem, E.; Oude Elberink, Joanna N.G.

Background: Venom immunotherapy (VIT) enables longtime prevention of insect venom allergy in the majority of patients. However, in some, the risk of a resystemic reaction increases after completion of treatment. No reliable factors predicting individual lack of efficacy of VIT are currently

Vitamin D enhances the efficacy of photodynamic therapy in a murine model of breast cancer

International Nuclear Information System (INIS)

Rollakanti, Kishore R; Anand, Sanjay; Maytin, Edward V

2015-01-01

Cutaneous metastasis occurs more frequently in breast cancer than in any other malignancy in women, causing significant morbidity. Photodynamic therapy (PDT), which combines a porphyrin-based photosensitizer and activation by light, can be employed for breast cancer (especially cutaneous metastases) but tumor control after PDT has not surpassed traditional treatments methods such as surgery, radiation, and chemotherapy up to now. Here, we report that breast cancer nodules in mice can be effectively treated by preconditioning the tumors with 1α, 25-dihydroxyvitamin D 3 (calcitriol; Vit D) prior to administering 5-aminolevulinate (ALA)-based PDT. Breast carcinoma tumors (MDA-MB-231 cells implanted subcutaneously in nude mice) received systemic Vit D (1 μg/kg) for 3 days prior to receiving ALA. The addition of Vit D increased intratumoral accumulation of protoporphyrin IX (PpIX) by 3.3 ± 0.5-fold, relative to mice receiving ALA alone. Bioluminescence imaging in vivo and immunohistochemical staining confirmed that tumor-specific cell death after ALA-PDT was markedly enhanced (36.8 ± 7.4-fold increase in TUNEL-positive nuclei; radiance decreased to 14% of control) in Vit D pretreated tumors as compared to vehicle-pretreated tumors. Vit D stimulated proliferation (10.7 ± 2.8-fold) and differentiation (9.62 ± 1.7-fold) in tumor cells, underlying an augmented cellular sensitivity to ALA-PDT. The observed enhancement of tumor responses to ALA-PDT after low, nontoxic doses of Vit D supports a new combination approach that deserves consideration in the clinical setting, and offers potential for improved remission of cutaneous breast cancer metastases

Assessing language and visuospatial functions with one task: a "dual use" approach to performing fMRI in children.

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Ebner, Kathina; Lidzba, Karen; Hauser, Till-Karsten; Wilke, Marko

2011-10-01

In order to increase the rate of successful functional MR studies in children it is helpful to shorten the time spent in the scanner. To this effect, assessing two cognitive functions with one task seems to be a promising approach. The hypothesis of this study was that the control condition of an established language task (vowel identification task, VIT) requires visuospatial processing and that the control condition (VIT(CC)) therefore may also be applicable to localize visuospatial functions. As a reference task, a visual search task (VST, previously established for use in children) was employed. To test this hypothesis, 43 children (19 f, 24 m; 12.0±2.6, range 7.9 to 17.8 years) were recruited and scanned using both tasks. Second-level random effects group analyses showed activation of left inferior-frontal cortex in the active condition of the VIT, as in previous studies. Additionally, analysis of the VIT(CC) demonstrated activation in right-dominant superior parietal and high-frontal brain regions, classically associated with visuospatial functions; activation seen in the VST was similar with a substantial overlap. However, lateralization in the parietal lobe was significantly more bilateral in the VST than in the VIT(CC). This suggests that the VIT can not only be applied to assess language functions (using the active>control contrast), but also that the control>active condition is useful for assessing visuospatial functions. Future task design may benefit from such a "dual use" approach to performing fMRI not only, but also particularly in children. Copyright © 2011 Elsevier Inc. All rights reserved.

Multifaceted remodeling by vitamin C boosts sensitivity of Mycobacterium tuberculosis subpopulations to combination treatment by anti-tubercular drugs.

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Sikri, Kriti; Duggal, Priyanka; Kumar, Chanchal; Batra, Sakshi Dhingra; Vashist, Atul; Bhaskar, Ashima; Tripathi, Kritika; Sethi, Tavpritesh; Singh, Amit; Tyagi, Jaya Sivaswami

2018-05-01

Bacterial dormancy is a major impediment to the eradication of tuberculosis (TB), because currently used drugs primarily target actively replicating bacteria. Therefore, decoding of the critical survival pathways in dormant tubercle bacilli is a research priority to formulate new approaches for killing these bacteria. Employing a network-based gene expression analysis approach, we demonstrate that redox active vitamin C (vit C) triggers a multifaceted and robust adaptation response in Mycobacterium tuberculosis (Mtb) involving ~ 67% of the genome. Vit C-adapted bacteria display well-described features of dormancy, including growth stasis and progression to a viable but non-culturable (VBNC) state, loss of acid-fastness and reduction in length, dissipation of reductive stress through triglyceride (TAG) accumulation, protective response to oxidative stress, and tolerance to first line TB drugs. VBNC bacteria are reactivatable upon removal of vit C and they recover drug susceptibility properties. Vit C synergizes with pyrazinamide, a unique TB drug with sterilizing activity, to kill dormant and replicating bacteria, negating any tolerance to rifampicin and isoniazid in combination treatment in both in-vitro and intracellular infection models. Finally, the vit C multi-stress redox models described here also offer a unique opportunity for concurrent screening of compounds/combinations active against heterogeneous subpopulations of Mtb. These findings suggest a novel strategy of vit C adjunctive therapy by modulating bacterial physiology for enhanced efficacy of combination chemotherapy with existing drugs, and also possible synergies to guide new therapeutic combinations towards accelerating TB treatment. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Venom immunotherapy improves health-related quality of life in patients allergic to yellow jacket venom

NARCIS (Netherlands)

Elberink, JNGO; de Monchy, JGR; van der Heide, S; Guyatt, GH; Dubois, AEJ

Background: Venom immunotherapy (VIT) is effective in preventing anaphylactic reactions after insect stings. The effect of VIT on health-related quality of life (HRQL) was studied to evaluate whether this treatment is of importance to patients. Objective: We compared HRQL outcomes measured with a

First successful case of in vitro fertilization-embryo transfer with venom immunotherapy for hymenoptera sting allergy

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Tucker Michael J

2004-10-01

Full Text Available Abstract Background To describe immune and endocrine responses in severe hymenoptera hypersensitivity requiring venom immunotherapy (VIT during in vitro fertilization (IVF. Case presentation A 39-year old patient was referred for history of multiple miscarriage and a history of insect sting allergy. Four years earlier, she began subcutaneous injection of 100 mcg mixed vespid hymenoptera venom/venom protein every 5–6 weeks. The patient had one livebirth and three first trimester miscarriages. Allergy treatment was maintained for all pregnancies ending in miscarriage, although allergy therapy was discontinued for the pregnancy that resulted in delivery. At our institution ovulation induction incorporated venom immunotherapy (VIT during IVF, with a reduced VIT dose when pregnancy was first identified. Serum IgE was monitored with estradiol during ovulation induction and early pregnancy. Response to controlled ovarian hyperstimulation was favorable while VIT was continued, with retrieval of 12 oocytes. Serum RAST (yellow jacket IgE levels fluctuated in a nonlinear fashion (range 36–54% during gonadotropin therapy and declined after hCG administration. A healthy female infant was delivered at 35 weeks gestation. The patient experienced no untoward effects from any medications during therapy. Conclusion Our case confirms the safety of VIT in pregnancy, and demonstrates RAST IgE can remain

Safety of specific immunotherapy using an ultra-rush induction regimen in bee and wasp allergy.

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Bożek, Andrzej; Kołodziejczyk, Krzysztof

2018-02-01

Specific allergen immunotherapy to Hymenoptera venom (VIT) is a basic treatment for patients allergic to Hymenoptera venom. The aim of the study was to evaluate the safety of an ultra-rush regimen compared with the rush and conventional protocols. In 31 patients with an allergy to bee venom and 82 with an allergy to wasp venom, the allergic adverse reactions during VIT were monitored. Patients were selected based on the criteria established by EAACI (European Academy of Allergy and Clinical Immunology) recommendations. Adverse reactions during the ultra-rush immunotherapy were measured, documented and classified according to the criteria of Mueller. Ultra-rush, rush or conventional protocols of the initial phase VIT using the Venomenhal vaccine (Hal Allergy, Leiden, Netherlands) were conducted. Six (13.7%) patients on the ultra-rush regimen, 5 (14.3%) patients on the rush regimen and 9 (26.5%) on conventional VIT experienced an allergic reaction. There were no associations between the adverse allergic reactions and the following factors: gender, total IgE and allergen-specific IgE to wasp or bee venom before the VIT and cardiological drugs that were used. We found that the ultra-rush protocol (similar to the rush protocol) using the Venomenhal vaccine is safer than the conventional protocol.

Does Vitamin C Influence Neurodegenerative Diseases and Psychiatric Disorders?

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Luchowska-Kocot, Dorota; Kiełczykowska, Małgorzata; Musik, Irena; Kurzepa, Jacek

2017-01-01

Vitamin C (Vit C) is considered to be a vital antioxidant molecule in the brain. Intracellular Vit C helps maintain integrity and function of several processes in the central nervous system (CNS), including neuronal maturation and differentiation, myelin formation, synthesis of catecholamine, modulation of neurotransmission and antioxidant protection. The importance of Vit C for CNS function has been proven by the fact that targeted deletion of the sodium-vitamin C co-transporter in mice results in widespread cerebral hemorrhage and death on post-natal day one. Since neurological diseases are characterized by increased free radical generation and the highest concentrations of Vit C in the body are found in the brain and neuroendocrine tissues, it is suggested that Vit C may change the course of neurological diseases and display potential therapeutic roles. The aim of this review is to update the current state of knowledge of the role of vitamin C on neurodegenerative diseases including Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, multiple sclerosis and amyotrophic sclerosis, as well as psychiatric disorders including depression, anxiety and schizophrenia. The particular attention is attributed to understanding of the mechanisms underlying possible therapeutic properties of ascorbic acid in the presented disorders. PMID:28654017

Homocyst(e)ine-lowering therapy does not affect plasma asymmetrical dimethylarginine concentrations in patients with peripheral artery disease.

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Ziegler, Sophie; Mittermayer, Friedrich; Plank, Christina; Minar, Erich; Wolzt, Michael; Schernthaner, Gerit-Holger

2005-04-01

Elevated plasma asymmetrical dimethylarginine (ADMA) is suggested to contribute to hyperhomocyst(e)ine-related vascular dysfunction in patients with peripheral artery disease (PAD). The present trial investigated whether homocyst(e)ine (Hcy)-lowering therapy with vitamin-B (vit-B) and folic acid affects plasma concentrations of ADMA in patients with PAD and hyperhomocyst(e)inemia. Forty-nine subjects (15 women, 34 men) with PAD and fasting plasma total Hcy concentrations greater than 15 micromol/liter were randomized to receive either oral vit-B and folic acid therapy (n = 27) or placebo (n = 22) for 6 wk. Fasting venous blood samples were monitored for plasma total Hcy, vit-B12 and folate, ADMA, symmetric dimethylarginine, L-arginine, and high-sensitivity C-reactive protein. After 6 wk, plasma Hcy concentrations were decreased, and concentrations of vit-B12 and folate were elevated in patients with vitamin supplementation (all P < 0.05 vs. baseline) and unchanged in the placebo group. Dimethylarginine plasma concentrations were not affected by treatment. High-sensitivity C-reactive protein correlated with ADMA plasma concentrations (r = 0.29; P < 0.01). The lack of vit-B and folic acid therapy on plasma concentrations of ADMA renders a role of extracellular methylarginines unlikely to be involved in the pathophysiology of hyperhomocyst(e)inemia and its complications.

Plasma 25-Hydroxyvitamin D and Risk of Non-Melanoma and Melanoma Skin Cancer

DEFF Research Database (Denmark)

Afzal, Shoaib; Nordestgaard, Børge G; Bojesen, Stig E

2013-01-01

Sun exposure is a major risk factor for skin cancer and is also an important source of vitamin D. We tested the hypothesis that elevated plasma 25-hydroxyvitamin D (25-OH-vitD) associates with increased risk of non-melanoma and melanoma skin cancer in the general population. We measured plasma 25......-OH-vitD in 10,060 white individuals from the Danish general population. During 28 years of follow-up, 590 individuals developed non-melanoma skin cancer and 78 developed melanoma skin cancer. Increasing 25-OH-vitD levels, by clinical categories or by seasonally adjusted tertiles, were associated...... with increasing cumulative incidence of non-melanoma skin cancer (trend P=2 × 10(-15) and P=3 × 10(-17)) and melanoma skin cancer (P=0.003 and P=0.001). Multivariable adjusted hazard ratios of non-melanoma skin cancer were 5.04 (95% confidence interval (CI): 2.78-9.16) for 25-OH-vitD 50 vs. 60 years, 25-OH...

Vitamin-C protect ethanol induced apoptotic neuro degeneration in postnatal rat brain

International Nuclear Information System (INIS)

Naseer, M.I.; Najeebullah; Ikramullah; Zubair, H.; Hassan, M.; Yang, B.C.

2010-01-01

Objective: To evaluate ethanol effects to induced activation of caspsae-3, and to observe the protective effects of Vitamin C (vit-C) on ethanol-induced apoptotic neuro degeneration in rat cortical area of brain. Methodology: Administration of a single dose of ethanol in 7-d postnatal (P7) rats triggers activation of caspase-3 and widespread apoptotic neuronal death. Western blot analysis, cells counting and Nissl staining were used to elucidate possible protective effect of vit-C against ethanol-induced apoptotic neuro degeneration in brain. Results: The results showed that ethanol significantly increased caspase-3 expression and neuronal apoptosis. Furthermore, the co-treatment of vit-C along with ethanol showed significantly decreased expression of caspase-3 as compare to control group. Conclusion: Our findings indicate that vit-C can prevent some of the deleterious effect of ethanol on developing rat brain when given after ethanol exposure and can be used as an effective protective agent for Fetal Alcohol Syndrome (FAS). (author)

1,25-(OH)2-vitamin D3 enhances the cytotoxic effect of radioiodine therapy in prostate cancer cells expressing the sodium iodide symporter

International Nuclear Information System (INIS)

Spitzweg, Christine; Hirschmann, Martin; Unterholzner, Stefanie; Cengic, Neziha; Eckel, Petra; Sharif-Samani, Bibi-Rana; Willhauck, Michael J.; Goeke, Burkhard; Morris, John C.

2005-01-01

Full text: We reported recently the induction of androgen-dependent iodide uptake activity in human prostate cancer cells (LNCaP) utilizing a prostate-specific antigen (PSA)-promoter directed expression of the sodium iodide symporter (NIS) gene. This offers the potential to treat prostate cancer with radioiodine. In the current study we examined the regulation of PSA-promoter directed NIS expression and therapeutic effectiveness of 131 I in LNCaP cells by 1,25-(OH)2-Vitamin D3 (Vit D3). For this purpose, NIS mRNA and protein expression levels in the NIS-transfected LNCaP cell line NP-1 were examined by Northern and Western blot analysis following incubation with Vit D3 (10 -9 M - 10 -5 M) in the presence of mibolerone (10 -9 M). In addition, NIS functional activity was measured by iodide uptake assay, and in vitro cytotoxicity of 131 I was examined by in vitro clonogenic assay. Following incubation with Vit D3, NIS mRNA levels in NP-1 cells were stimulated 1.2-fold, whereas NIS protein levels increased 1.65-fold and iodide accumulation was stimulated 1.4-fold in a concentration-dependent manner. Further, the selective killing effect of 131 I in NP-1 cells was significantly increased from 55% in NP-1 cells incubated with mibolerone alone to 86 % in NP-1 cells treated with Vit D3 (10 -5 M) in the presence of mibolerone. In the absence of androgen, with or without Vit D3 no functional NIS expression was detected. Conclusion: Treatment with Vit D3 increases androgen-induced NIS expression levels and selective killing effect of 131 I in prostate cancer cells stably expressing NIS under the control of the PSA promoter. Vit D3 may therefore be used to enhance the therapeutic response to radioiodine in prostate cancer cells following PSA-promoter directed NIS gene delivery. (author)

Vascular Impulse Technology versus elevation in the treatment of posttraumatic swelling of extremity fractures: study protocol for a randomized controlled trial.

Science.gov (United States)

Schnetzke, Marc; Swartman, Benedict; Bonnen, Isabel; Keil, Holger; Schüler, Svenja; Grützner, Paul A; Franke, Jochen

2017-02-16

Fractures of the extremities are often complicated by a variable degree of swelling secondary to hemorrhage and soft tissue injury. Patients typically require up to 7 days of inpatient bed rest and elevation to reduce swelling to an acceptable level for operative treatment with internal fixation. Alternatively, an intermittent pneumatic compression device, such as the Vascular Impulse Technology (VIT) system, can be used at the injured extremity to reduce the posttraumatic swelling. The VIT system consists of a pneumatic compressor that intermittently rapidly inflates a bladder positioned under the arch of the hand or the foot, which results in compression of the venous hand or foot plexus. That intermittent compression induces an increased venous velocity and aims to reduce the soft tissue swelling of the affected extremity. The VIT study is a prospective, monocenter, randomized controlled trial to compare the VIT system with elevation in the treatment of posttraumatic swelling in the case of a fracture of the upper and lower extremity. This study will include 280 patients with fractures of the upper and the lower extremity with nine different injury types. For each of the nine injury types a separate randomization to the two intervention groups (VIT group or control group) will be performed. The primary outcome parameter is the time taken for the swelling to resolve sufficiently to permit surgery. A separate analysis for each of the nine injury types will be performed. In the proposed study, the effectiveness of the VIT system in the treatment of posttraumatic swelling of upper and lower extremity fractures will be evaluated. German Clinical Trial Register, No. DRKS00010510 . Registered on 17 July 2016.

Changes in Non-Enzymatic Antioxidants in the Blood Following Anaerobic Exercise in Men and Women

Science.gov (United States)

Wiecek, Magdalena; Kantorowicz, Malgorzata

2015-01-01

Purpose The aim of this study was to compare changes in total oxidative status (TOS), total antioxidative capacity (TAC) and the concentration of VitA, VitE, VitC, uric acid (UA), reduced (GSH) and oxidized glutathione (GSSG) in blood within 24 hours following anaerobic exercise (AnEx) among men and women. Methods 10 women and 10 men performed a 20-second bicycle sprint (AnEx). Concentrations of oxidative stress indicators were measured before AnEx and 3, 15 and 30 minutes and 1 hour afterwards. UA, GSH and GSSH were also measured 24 hours after AnEx. Lactate and H+ concentrations were measured before and 3 minutes after AnEx. Results The increase in lactate and H+ concentrations following AnEx was similar in both sexes. Changes in the concentrations of all oxidative stress indicators were significant and did not differ between men and women. In both sexes, TOS, TAC, TOS/TAC and VitA and VitE concentrations were the highest 3 minutes, VitC concentration was the highest 30 minutes, and UA concentration was the highest 1 hour after AnEx. GSH concentration was significantly lower than the initial concentration from 15 minutes to 24 hour after AnEx. GSSG concentration was significantly higher, while the GSH/GSSG ratio was significantly lower than the initial values 1 hour and 24 hour after AnEx. Conclusions With similar changes in lactate and H+ concentrations, AnEx induces the same changes in TAC, TOS, TOS/TAC and non-enzymatic antioxidants of low molecular weight in men and women. Oxidative stress lasted at least 24 hours after AnEx. PMID:26600020

Immunological differences between insect venom-allergic patients with and without immunotherapy and asymptomatically sensitized subjects.

Science.gov (United States)

Arzt, L; Bokanovic, D; Schrautzer, C; Laipold, K; Möbs, C; Pfützner, W; Herzog, S A; Vollmann, J; Reider, N; Bohle, B; Aberer, W; Sturm, G J

2017-11-23

Currently available tests are unable to distinguish between asymptomatic sensitization and clinically relevant Hymenoptera venom allergy. A reliable serological marker to monitor venom immunotherapy (VIT) does also not exist. Our aim was to find reliable serological markers to predict tolerance to bee and vespid stings. We included 77 asymptomatically sensitized subjects, 85 allergic patients with acute systemic sting reactions, and 61 allergic patients currently treated with VIT. Levels of sIgE and sIgG 4 to bee and vespid venom, rApi m 1, and rVes v 5 were measured immediately after allergic sting reactions or before sting challenges and 4 weeks later. All sting challenges were tolerated. The inhibitory activity was determined using BAT inhibition and ELIFAB assay. Median sIgG 4 levels were 96-fold higher in VIT patients (P venom, but not in those treated with bee venom. Four weeks after the sting, sIgE and sIgG 4 levels were increased in allergic and asymptomatically sensitized patients, but not in VIT patients. Immunological responses after stings varied in bee and vespid venom-allergic patients. In patients under VIT, sIgE and sIgG 4 remained completely stable after sting challenges. Monitoring VIT efficacy was only possible in vespid venom allergy, and the sIgG 4 threshold for rVes v 5 had the highest sensitivity to confirm tolerance. The BAT inhibition test was the most reliable tool to confirm tolerance on an individual basis. © 2017 EAACI and John Wiley and Sons A/S. Published by John Wiley and Sons Ltd.

Correlation between follicular fluid levels of sRAGE and vitamin D in women with PCOS.

Science.gov (United States)

Garg, Deepika; Grazi, Richard; Lambert-Messerlian, Geralyn M; Merhi, Zaher

2017-11-01

The pro-inflammatory advanced glycation end products (AGEs) and their anti-inflammatory soluble receptors, sRAGE, play a role in the pathogenesis of PCOS. There is a correlation between vitamin D (vit D) and sRAGE in the serum, whereby vit D replacement increases serum sRAGE levels in women with PCOS, thus incurring a protective anti-inflammatory role. This study aims to compare levels of sRAGE, N-carboxymethyl-lysine (CML; one of the AGEs), and 25-hydroxy-vit D in the follicular fluid (FF) of women with or without PCOS, and to evaluate the correlation between sRAGE and 25-hydroxy-vit D in the FF. Women with (n = 12) or without (n = 13) PCOS who underwent IVF were prospectively enrolled. Women with PCOS had significantly higher anti-Mullerian hormone levels, higher number of total retrieved and mature oocytes, and higher number of day 3 and day 5 embryos formed. Compared to women without PCOS, women with PCOS had significantly lower FF sRAGE levels. In women with PCOS, in women without PCOS, and in all participants together, there was a significant positive correlation between sRAGE and 25-hydroxy-vit D. sRAGE positively correlated with CML in women without PCOS but not in women with PCOS. In women with PCOS, the low ovarian levels of the anti-inflammatory sRAGE suggest that sRAGE could represent a biomarker and a potential therapeutic target for ovarian dysfunction in PCOS. Whether there is a direct causal relationship between sRAGE and vit D in the ovaries remains to be determined.

Non-invasive assessment of intratumoral vascularity using arterial spin labeling: A comparison to susceptibility-weighted imaging for the differentiation of primary cerebral lymphoma and glioblastoma

International Nuclear Information System (INIS)

Furtner, J.; Schöpf, V.; Preusser, M.; Asenbaum, U.; Woitek, R.; Wöhrer, A.; Hainfellner, J.A.; Wolfsberger, S.; Prayer, D.

2014-01-01

Using conventional MRI methods, the differentiation of primary cerebral lymphomas (PCNSL) and other primary brain tumors, such as glioblastomas, is difficult due to overlapping imaging characteristics. This study was designed to discriminate tumor entities using normalized vascular intratumoral signal intensity values (nVITS) obtained from pulsed arterial spin labeling (PASL), combined with intratumoral susceptibility signals (ITSS) from susceptibility-weighted imaging (SWI). Thirty consecutive patients with glioblastoma (n = 22) and PCNSL (n = 8), histologically classified according to the WHO brain tumor classification, were included. MRIs were acquired on a 3 T scanner, and included PASL and SWI sequences. nVITS was defined by the signal intensity ratio between the tumor and the contralateral normal brain tissue, as obtained by PASL images. ITSS was determined as intratumoral low signal intensity structures detected on SWI sequences and were divided into four different grades. Potential differences in the nVITS and ITSS between glioblastomas and PCNSLs were revealed using statistical testing. To determine sensitivity, specificity, and diagnostic accuracy, as well as an optimum cut-off value for the differentiation of PCNSL and glioblastoma, a receiver operating characteristic analysis was used. We found that nVITS (p = 0.011) and ITSS (p = 0.001) values were significantly higher in glioblastoma than in PCNSL. The optimal cut-off value for nVITS was 1.41 and 1.5 for ITSS, with a sensitivity, specificity, and accuracy of more than 95%. These findings indicate that nVITS values have a comparable diagnostic accuracy to ITSS values in differentiating glioblastoma and PCNSL, offering a completely non-invasive and fast assessment of tumoral vascularity in a clinical setting

Non-invasive assessment of intratumoral vascularity using arterial spin labeling: A comparison to susceptibility-weighted imaging for the differentiation of primary cerebral lymphoma and glioblastoma.

Science.gov (United States)

Furtner, J; Schöpf, V; Preusser, M; Asenbaum, U; Woitek, R; Wöhrer, A; Hainfellner, J A; Wolfsberger, S; Prayer, D

2014-05-01

Using conventional MRI methods, the differentiation of primary cerebral lymphomas (PCNSL) and other primary brain tumors, such as glioblastomas, is difficult due to overlapping imaging characteristics. This study was designed to discriminate tumor entities using normalized vascular intratumoral signal intensity values (nVITS) obtained from pulsed arterial spin labeling (PASL), combined with intratumoral susceptibility signals (ITSS) from susceptibility-weighted imaging (SWI). Thirty consecutive patients with glioblastoma (n=22) and PCNSL (n=8), histologically classified according to the WHO brain tumor classification, were included. MRIs were acquired on a 3T scanner, and included PASL and SWI sequences. nVITS was defined by the signal intensity ratio between the tumor and the contralateral normal brain tissue, as obtained by PASL images. ITSS was determined as intratumoral low signal intensity structures detected on SWI sequences and were divided into four different grades. Potential differences in the nVITS and ITSS between glioblastomas and PCNSLs were revealed using statistical testing. To determine sensitivity, specificity, and diagnostic accuracy, as well as an optimum cut-off value for the differentiation of PCNSL and glioblastoma, a receiver operating characteristic analysis was used. We found that nVITS (p=0.011) and ITSS (p=0.001) values were significantly higher in glioblastoma than in PCNSL. The optimal cut-off value for nVITS was 1.41 and 1.5 for ITSS, with a sensitivity, specificity, and accuracy of more than 95%. These findings indicate that nVITS values have a comparable diagnostic accuracy to ITSS values in differentiating glioblastoma and PCNSL, offering a completely non-invasive and fast assessment of tumoral vascularity in a clinical setting. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

Non-invasive assessment of intratumoral vascularity using arterial spin labeling: A comparison to susceptibility-weighted imaging for the differentiation of primary cerebral lymphoma and glioblastoma

Energy Technology Data Exchange (ETDEWEB)

Furtner, J., E-mail: julia.furtner@meduniwien.ac.at [Department of Biomedical Imaging und Image-guided Therapy, Medical University of Vienna (Austria); Comprehensive Cancer Center-Central Nervous System Tumors Unit (CCC-CNS), Medical University of Vienna (Austria); Schöpf, V., E-mail: veronika.schoepf@meduniwien.ac.at [Department of Biomedical Imaging und Image-guided Therapy, Medical University of Vienna (Austria); Preusser, M., E-mail: matthias.preusser@meduniwien.ac.at [Department of Medicine I, Division of Oncology, Medical University of Vienna (Austria); Comprehensive Cancer Center-Central Nervous System Tumors Unit (CCC-CNS), Medical University of Vienna (Austria); Asenbaum, U., E-mail: ulrika.asenbaum@meduniwien.ac.at [Department of Biomedical Imaging und Image-guided Therapy, Medical University of Vienna (Austria); Woitek, R., E-mail: ramona.woitek@meduniwien.ac.at [Department of Biomedical Imaging und Image-guided Therapy, Medical University of Vienna (Austria); Wöhrer, A., E-mail: adelheid.woehrer@meduniwien.ac.at [Institute of Neurology, Medical University of Vienna (Austria); Comprehensive Cancer Center-Central Nervous System Tumors Unit (CCC-CNS), Medical University of Vienna (Austria); Hainfellner, J.A., E-mail: johannes.hainfellner@meduniwien.ac.at [Institute of Neurology, Medical University of Vienna (Austria); Comprehensive Cancer Center-Central Nervous System Tumors Unit (CCC-CNS), Medical University of Vienna (Austria); Wolfsberger, S., E-mail: stefan.wolfsberger@meduniwien.ac.at [Department of Neurosurgery, Medical University of Vienna (Austria); Comprehensive Cancer Center-Central Nervous System Tumors Unit (CCC-CNS), Medical University of Vienna (Austria); Prayer, D., E-mail: daniela.prayer@meduniwien.ac.at [Department of Biomedical Imaging und Image-guided Therapy, Medical University of Vienna (Austria); Comprehensive Cancer Center-Central Nervous System Tumors Unit (CCC-CNS), Medical University of Vienna (Austria)

2014-05-15

Using conventional MRI methods, the differentiation of primary cerebral lymphomas (PCNSL) and other primary brain tumors, such as glioblastomas, is difficult due to overlapping imaging characteristics. This study was designed to discriminate tumor entities using normalized vascular intratumoral signal intensity values (nVITS) obtained from pulsed arterial spin labeling (PASL), combined with intratumoral susceptibility signals (ITSS) from susceptibility-weighted imaging (SWI). Thirty consecutive patients with glioblastoma (n = 22) and PCNSL (n = 8), histologically classified according to the WHO brain tumor classification, were included. MRIs were acquired on a 3 T scanner, and included PASL and SWI sequences. nVITS was defined by the signal intensity ratio between the tumor and the contralateral normal brain tissue, as obtained by PASL images. ITSS was determined as intratumoral low signal intensity structures detected on SWI sequences and were divided into four different grades. Potential differences in the nVITS and ITSS between glioblastomas and PCNSLs were revealed using statistical testing. To determine sensitivity, specificity, and diagnostic accuracy, as well as an optimum cut-off value for the differentiation of PCNSL and glioblastoma, a receiver operating characteristic analysis was used. We found that nVITS (p = 0.011) and ITSS (p = 0.001) values were significantly higher in glioblastoma than in PCNSL. The optimal cut-off value for nVITS was 1.41 and 1.5 for ITSS, with a sensitivity, specificity, and accuracy of more than 95%. These findings indicate that nVITS values have a comparable diagnostic accuracy to ITSS values in differentiating glioblastoma and PCNSL, offering a completely non-invasive and fast assessment of tumoral vascularity in a clinical setting.

Vitamin D3 targets epidermal and dermal dendritic cells for induction of distinct regulatory T cells

NARCIS (Netherlands)

van der Aar, Angelic M. G.; Sibiryak, Darya S.; Bakdash, Ghaith; van Capel, Toni M. M.; van der Kleij, Hanneke P. M.; Opstelten, Dirk-Jan E.; Teunissen, Marcel B. M.; Kapsenberg, Martien L.; de Jong, Esther C.

2011-01-01

Background: The vitamin D metabolite 1,25(OH) 2D3 (VitD3) is a potent immunosuppressive drug and, among others, is used for topical treatment of psoriasis. A proposed mechanism of VitD3-mediated suppression is priming of dendritic cells (DCs) to induce regulatory T (Treg) cells. Objective:

Intradermal application of vitamin D3 increases migration of CD14+ dermal dendritic cells and promotes the development of Foxp3+ regulatory T cells

NARCIS (Netherlands)

Bakdash, Ghaith; Schneider, Laura P.; van Capel, Toni M. M.; Kapsenberg, Martien L.; Teunissen, Marcel B. M.; de Jong, Esther C.

2013-01-01

The active form of vitamin D3 (VitD) is a potent immunosuppressive drug. Its effects are mediated in part through dendritic cells (DCs) that promote the development of regulatory T cells (Tregs). However, it remains elusive how VitD would influence the different human skin DC subsets, e.g.,

Effects of Docosahexaenoic Supplementation and In Vitro Vitamin C on the Oxidative and Inflammatory Neutrophil Response to Activation

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Xavier Capó

2015-01-01

Full Text Available We studied the effects of diet supplementation with docosahexaenoic (DHA and in vitro vitamin C (VitC at physiological concentrations on oxidative and inflammatory neutrophil response to phorbol myristate acetate (PMA. Fifteen male footballers ingested a beverage enriched with DHA or a placebo for 8 weeks in a randomized double-blind study. Neutrophils were isolated from blood samples collected in basal conditions at the end of nutritional intervention. Neutrophils were cultured for 2 hours at 37°C in (a control media, (b media with PMA, and (c media with PMA + VitC. PMA induces neutrophil degranulation with increased extracellular myeloperoxidase and catalase activities, nitric oxide production, expression of the inflammatory genes cyclooxygenase-2, nuclear factor κβ, interleukin 8 and tumor necrosis factor α, and interleukin 6 production. DHA diet supplementation boosts the exit of CAT from neutrophils but moderates the degranulation of myeloperoxidase granules induced by PMA. VitC facilitates azurophilic degranulation of neutrophils and increases gene expression of myeloperoxidase induced by PMA. VitC and DHA diet supplementation prevent PMA effects on inflammatory gene expression, although together they do not produce additional effects. DHA diet supplementation enhances antioxidant defences and anti-inflammatory neutrophil response to in vitro PMA activation. VitC facilitates neutrophil degranulation but prevents an inflammatory response to PMA.

Plasma Vitamin E and Blood Selenium Concentrations in Norwegian Dairy Cows: Regional Differences and Relations to Feeding and Health

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Østerås O

2005-12-01

Full Text Available Plasma α-tocopherol (vit E and blood selenium (Se concentrations in February were determined in samples from 314 dairy cows in Norway, selected to provide a representative subset of the Norwegian dairy cow population. Each sample was followed by a questionnaire with information about feeding of the cow at the time of sampling. The results were correlated to herd data and to calving and health data for each cow from the Norwegian Dairy Herd Recording System and the Norwegian Cattle Health Recording System. The mean concentrations were 6.9 μg vit E per ml plasma and 0.16 μg Se per g blood. Both levels were highest in mid lactation. Plasma vit E varied with the amount of silage fed to the cow, while blood Se varied with the amount of concentrates and mineral supplements, and with geographical region. No differences in vit E or Se levels were found between cows with recorded treatments for mastitis, parturient paresis or reproductive disorders in the lactation during or immediately prior to sampling, and those without such treatments. For ketosis, a small difference in blood Se was found between the groups with or without recorded treatments. It is concluded that winter-fed lactating cows in Norway had an adequate plasma level of vit E and a marginal-to-adequate level of Se.

Plasma Vitamin E and Blood Selenium Concentrations in Norwegian Dairy Cows: Regional Differences and Relations to Feeding and Health

Directory of Open Access Journals (Sweden)

Sivertsen T

2006-12-01

Full Text Available Plasma α-tocopherol (vit E and blood selenium (Se concentrations in February were determined in samples from 314 dairy cows in Norway, selected to provide a representative subset of the Norwegian dairy cow population. Each sample was followed by a questionnaire with information about feeding of the cow at the time of sampling. The results were correlated to herd data and to calving and health data for each cow from the Norwegian Dairy Herd Recording System and the Norwegian Cattle Health Recording System. The mean concentrations were 6.9 μg vit E per ml plasma and 0.16 μg Se per g blood. Both levels were highest in mid lactation. Plasma vit E varied with the amount of silage fed to the cow, while blood Se varied with the amount of concentrates and mineral supplements, and with geographical region. No differences in vit E or Se levels were found between cows with recorded treatments for mastitis, parturient paresis or reproductive disorders in the lactation during or immediately prior to sampling, and those without such treatments. For ketosis, a small difference in blood Se was found between the groups with or without recorded treatments. It is concluded that winter-fed lactating cows in Norway had an adequate plasma level of vit E and a marginal-to-adequate level of Se.

Protective effects of vitamin E and Cornus mas fruit extract on methotrexate-induced cytotoxicity in sperms of adult mice

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Leila Zarei

2014-03-01

Full Text Available This study was aimed to assess the protective effects of Cornus mas fruit extract (CMFE and vitamin E (Vit E on sperm quality parameters in the methotrexate (MTX-treated mice. Forty-eight young adult male mice (8-12 weeks were randomly divided into six groups including control and test groups. The control group received normal saline orally , and the test groups were treated MTX (20 mg kg-1, ip, once weekly, MTX + CMFE (250 mg kg-1, MTX + CMFE (500 mg kg-1, MTX + CMFE (1000 mg kg-1, and MTX + Vit E (100 IU kg-1, po for 35 consecutive days. On day 35, after euthanasia the epididymal sperms were isolated. Then the total mean sperm count, sperm viability and motility were determined. The total antioxidant capacity (TAOC of all experimental groups were also evaluated. The MTX-treated animals showed a significant changes in all parameters of sperm quality assessment compared to the control group. Both Vit E and CMFE were able to protect from MTX-induced effects on sperm maturity and DNA damage. Co-administration of MTX and CMFE and/or Vit E resulted in protection from MTX-reduced TAOC. In conclusion, these data suggested that MTX administration could adversely affect the sperm quality. Moreover, the protective effect of Vit E and CMFE on MTX-induced sperm toxicity was also documented.

The impact of electromagnetic radiation (2.45 GHz, Wi-Fi) on the female reproductive system: The role of vitamin C.

Science.gov (United States)

Saygin, Mustafa; Ozmen, Ozlem; Erol, Onur; Ellidag, Hamit Yasar; Ilhan, Ilter; Aslankoc, Rahime

2018-01-01

The present study investigated the effects of applied continuous 2.45 GHz electromagnetic radiation (EMR), which might cause physiopathological or morphological changes in the ovarian, fallopian tubal, and uterine tissues of rats. We proposed that the addition of vitamin C (Vit C) may reduce these severe effects. Eighteen female Sprague Dawley rats were randomly divided into three groups with six animals in each: Sham, EMR (EMR, 1 h/day for 30 days), and EMR + Vit C (EMR, 1 h/day for 30 days 250 mg/kg/daily). Total oxidant status (TOS) and oxidative stress index (OSI) levels increased ( p = 0.011 and p = 0.002, respectively) in the EMR-only group in ovarian tissues. In all tissues, TOS and OSI levels significantly decreased in the Vit C-treated group in ovarian, fallopian tubal, and uterine tissues ( p < 0.05). Anti-müllerian hormone levels significantly increased in the EMR group ( p < 0.05) and decreased in the Vit C-treated groups. Estrogen (E2) levels were unchanged in the EMR group, as the differences were not statistically significant. Immunohistochemical examination of the ovaries revealed significant increases in Caspase-3 expressions in the epithelial cells of the EMR group ( p < 0.05). In the EMR group, hyperemia was observed in uterine tissues. Also, Caspase-3 and Caspase-8 were significantly increased in the EMR group ( p < 0.001). Caspase-3 was significantly diminished with Vit C application in the ovarian and uterine tissues ( p < 0.05). Caspase-8 was significantly diminished only in uterine tissues ( p < 0.05). These results indicate that prolonged EMR exposure induced physiopathological changes in the ovarian, fallopian tubal, and uterine tissues due to oxidative damage. Under the conditions of this study, Vit C may have protective effects on female reproductive system against oxidative damage.

Techniques for capturing bighorn sheep lambs

Science.gov (United States)

Smith, Joshua B.; Walsh, Daniel P.; Goldstein, Elise J.; Parsons, Zachary D.; Karsch, Rebekah C.; Stiver, Julie R.; Cain, James W.; Raedeke, Kenneth J.; Jenks, Jonathan A.

2014-01-01

Low lamb recruitment is a major challenge facing managers attempting to mitigate the decline of bighorn sheep (Ovis canadensis), and investigations into the underlying mechanisms are limited because of the inability to readily capture and monitor bighorn sheep lambs. We evaluated 4 capture techniques for bighorn sheep lambs: 1) hand-capture of lambs from radiocollared adult females fitted with vaginal implant transmitters (VITs), 2) hand-capture of lambs of intensively monitored radiocollared adult females, 3) helicopter net-gunning, and 4) hand-capture of lambs from helicopters. During 2010–2012, we successfully captured 90% of lambs from females that retained VITs to ≤1 day of parturition, although we noted differences in capture rates between an area of high road density in the Black Hills (92–100%) of South Dakota, USA, and less accessible areas of New Mexico (71%), USA. Retention of VITs was 78% with pre-partum expulsion the main cause of failure. We were less likely to capture lambs from females that expelled VITs ≥1 day of parturition (range = 80–83%) or females that were collared without VITs (range = 60–78%). We used helicopter net-gunning at several sites in 1999, 2001–2002, and 2011, and it proved a useful technique; however, at one site, attempts to capture lambs led to lamb predation by golden eagles (Aquila chrysaetos). We attempted helicopter hand-captures at one site in 1999, and they also were successful in certain circumstances and avoided risk of physical trauma from net-gunning; however, application was limited. In areas of low accessibility or if personnel lack the ability to monitor females and/or VITs for extended periods, helicopter capture may provide a viable option for lamb capture.

Vitamin K2 alleviates type 2 diabetes in rats by induction of osteocalcin gene expression.

Science.gov (United States)

Hussein, Atef G; Mohamed, Randa H; Shalaby, Sally M; Abd El Motteleb, Dalia M

2018-03-01

The biological mechanisms behind the association between vitamin K (Vit K) and glucose metabolism are uncertain. We aimed to analyze the expression of insulin 1 (Ins 1), insulin 2 (Ins 2) and cyclin D2, the expression of adiponectin and UCP-1 . In addition, we aimed to estimate the doses of Vit K2 able to affect various aspects of glucose and energy metabolism in type 2 diabetes. Thirty adult male rats were allocated equally into five groups: control group, diabetes mellitus group, and groups 3, 4, and 5, which received Vit K 2 at three daily dose levels (10, 15, and 30 mg/kg, respectively) for 8 wk. At the end of the study, blood samples were collected to quantify total osteocalcin, fasting plasma glucose, fasting insulin, and relevant variables. The expression of OC, Ins 1, Ins 2, cyclin D2, adiponectin, UCP-1 genes was analyzed by real-time polymerase chain reaction. After administration of Vit K 2 , a dose-dependent decrease in fasting plasma glucose, hemoglobin A1c and homeostatic model assessment method insulin resistance, and a dose-dependent increase in fasting insulin and homeostatic model assessment method β cell function levels, when compared with diabetes mellitus rats, were detected. There was significant upregulation of OC, Ins 1, Ins 2, or cyclin D2 gene expression in the three treated groups in a dose-dependent manner when compared with the diabetic rats. However, expression of adiponectin and UCP-1 were significantly increased at the highest dose (30 mg/kg daily) only. Vit K 2 administration could improve glycemic status in type 2 diabetic rats by induction of OC gene expression. Osteocalcin could increase β-cell proliferation, energy expenditure, and adiponectin expression. Different concentrations of Vit K 2 were required to affect glucose metabolism and insulin sensitivity. Copyright © 2017 Elsevier Inc. All rights reserved.

Antioxidants as recipes for efavirenz-induced liver damage: A study in albino rats

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Elias Adikwu

2018-03-01

Full Text Available Objective: Hepatotoxicity is a clinical challenge associated with the use of efavirenz (EFV. This study investigated the effects of n-acetylcysteine (NAC, vitamins C and E on EFV-induced hepatotoxicity in albino rats. Methods: Rats were divided into groups and administered with NAC (20mg/kg, Vit C (50mg/kg, Vit  E (50mg/kg, Vit C+ E and 60mg/kg of EFV respectively. Rats were also divided into groups and pretreated with NAC, Vit C, E, and combined doses of Vit C+E prior to treatment with EFV for 15 days respectively. After drug administration rats were sacrificed and serum was collected and evaluated for liver function parameters. Rats were dissected, liver was collected weighed and evaluated for alkaline phosphatase (ALP, alanine aminotransferase (AST, aspartate aminotransferase (ALT, gamma glutamyl transferase (GGT, lactate dehydrogenase (LDH, malondialdehyde (MDA, super oxide dismutase (SOD, catalase (CAT, glutathione (GSH, gluthatione peroxidase (GPX levels and pathological damage. Results: Effects were not significant (p>0.05 on body and liver weights, however, the levels of AST, ALT, AST, GGT, LDH, CB, TB and MDA were increased significantly (p<0.05 whereas SOD, CAT, SOD, GSH and GPX were decreased significantly (p<0.05 in EFV-treated rats in comparison to control. The liver of EFV-treated rats showed necrosis of hepatocytes. Nevertheless, EFV-induced alterations in the above parameters were significantly (p<0.05 ameliorated in antioxidants pretreated rats.  The combined doses of Vit C and E produced the best and significant (p<0.05 ameliorative effects in comparison to their individual doses. Conclusion: This study shows the prospects of antioxidants as candidates for the treatments of efavirenz-induced hepatotoxicity.

Stinging insect allergy: current perspectives on venom immunotherapy

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Ludman SW

2015-07-01

Full Text Available Sian W Ludman,1 Robert J Boyle2 1Paediatric Allergy Department, St Mary's Hospital, Imperial Healthcare NHS Trust, London, UK; 2Department of Paediatrics, Imperial College London, London, UKAbstract: Systemic allergic reactions to insect stings affect up to 5% of the population during their lifetime, and up to 32% of beekeepers. Such reactions can be fatal, albeit very rarely, and fear of a further systemic reaction (SR can lead to significant anxiety and quality of life impairment. A recent Cochrane systematic review confirmed that venom immunotherapy (VIT is an effective treatment for people who have had a systemic allergic reaction to an insect sting. VIT reduces risk of a further SR (relative risk 0.10, 95% confidence interval 0.03–0.28, but VIT also reduces risk of a future large local reaction, and significantly improves disease-specific quality of life. However, health economic analysis showed that VIT is generally not cost effective for preventing future SRs; most people are stung infrequently, most SRs resolve without long-term consequences, and a fatal outcome is extremely rare. VIT only becomes cost effective if one is stung frequently (eg, beekeepers or if quality of life improvement is considered. Thus, for most people with insect sting allergy, anxiety and quality of life impairment should be the overriding consideration when making treatment decisions, highlighting the importance of a patient-centered approach. Areas which need to be explored in future research include efforts to improve the safety and convenience of VIT such as the use of sublingual immunotherapy; quality of life effects of venom allergy in children and adolescents as well as their parents; and the optimal duration of treatment.Keywords: anaphylaxis, quality of life

Effect of oral alpha-tocopherol and zinc on plasma status, IGF-I levels, weight gain and immune response in young calves

DEFF Research Database (Denmark)

Sehested, J.; Jørgensen, C.; Mortensen, S.B.

2004-01-01

IGF-I levels generally increased from birth to week 8, but were not affected by Zn or vit. E. Daily weight gain was highest in the Zn group, but this was not related to an increased IGF-I level. The vit. E group responded earlier than the Zn and control groups to the tetanus vaccination....

Experimental studies on anti-oxidants reducing lipid peroxidation of irradiated mice

International Nuclear Information System (INIS)

Du Zeji; Liu Keliang; Su Liaoyuan

1993-08-01

The free radical plays an important role in the irradiation damage. The irradiation damage would be reduced if anti-oxidants is used, because anti-oxidants can scavenge free radicals and suppress lipid peroxidation. In the study, a fluoro-spectrophotometer was used to determine the changes of MDA levels in mice tissues and serum after irradiation and the protective effect of anti-oxidants of Vit E and DMSO on damage caused by free radicals. The results are as follows: (1) The highest MDA level was at 12 to 24 hours after irradiation dose of 3.0 Gy. (2) The MDA level is increasing with the increasing of irradiation dose. It means the MDA level can indicate the extent of irradiation damage. (3) Both Vit E and DMSO had a powerful effect on reducing MDA level, but the effect of DMSO was stronger than Vit E. The optimum doses of them were 0.25 mg/g body weight and 10 mg/g body weight respectively. (4) The best effect obtained was to use Vit E and DMSO simultaneously

POSICIONAMENTO ESTRATÉGICO DE UMA ORGANIZAÇÃO HOSPITALAR SOB A ÓTICA DA ORGANIZAÇÃO INDUSTRIAL

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Luiz Claudio Louzada

2015-11-01

Full Text Available Este artigo aplica o arcabouço da Organização Industrial no setor hospitalar da Grande Vitória. Utiliza-se do paradigma Estrutura-Conduta e Desempenho, desenvolvido por Scherer e Ross (1990, para mapear a estrutura e analisar o ambiente de concorrência no setor hospitalar da grande vitória.  Na estruturação do trabalho foi utilizada seguinte seqüência de análise: (1 análise sócio-economica; (2 evolução do setor; e, (3 ambiente de concorrência.  Por conseguinte são descritos os fatores predominantes no setor hospitalar, baseado no modelo de Michael Porter (1986, os quais serviram para posicionar o HSP1 no setor hospitalar da Grande Vitória. O resultado da análise consiste em uma sugestão de três focos estratégicos possíveis para o reposicionamento do HSP1 no mercado da Grande Vitória, destacando em cada uma das opções as principais vantagens e desvantagens.

Comparing the Effect of Oral Supplementation of Vitamin E, Injective Vitamin E and Selenium or Both during Late Pregnancy on Production and Reproductive Performance and Immune Function of Dairy Cows and Calves

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Farokh Kafilzadeh

2014-01-01

Full Text Available The object of this study was to determine the effect of prepartum supplementation of vitamin E with or without injective vitamin E and selenium (Se on productive and reproductive performances and immune function in dairy cows. Sixty multiparous Holstein dairy cows were divided randomly into three groups at the end of gestation. Cows in each group received one of three treatments: (1 a single intramuscular (im injection of vit. E + selenium 3 weeks prepartum; (2 daily supplementation of oral vit. E given from 3 weeks prepartum to parturition; (3 injective vit. E + Se with daily supplementation of oral vit. E. Blood samples were collected from cows at calving and from calves at 0 and 7 days of age. Concentration of IgG in serum of cows and calves as well as in colostrum was determined. No significant differences among treatments occurred in the concentrations of IgG, animal, and calf production and reproduction performance. Due to the lack of significant difference between injection and oral supplementation, it is recommended to replace the injection with oral supplementation.

Interactions of ozone and antineoplastic drugs on rat lung fibroblasts and Walker rat carcinoma cells

International Nuclear Information System (INIS)

Wenzel, D.G.; Morgan, D.L.

1983-01-01

Cultured rat lung fibroblasts (F-cells) and Walker rat carcinoma cells (WRC-cells) labeled with 51 Cr were exposed to the following antitumor drugs alone or with O 3 : carmustine (BCNU), doxorubicin (Dox), cisplatin (CPt), mitomycin C (Mit C) or vitamin K 3 (Vit K). Release of 51 Cr (cell injury) was greater for F-cells than WRC-cells with any single treatment. Pretreatment with any drug (400 microM), except for Vit K with WRC-cells, did not significantly increase O 3 -induced loss of 51 Cr. Co-exposure of F-cells to drugs and O 3 resulted in a marked potentiation of O 3 -induced injury with Vit K, and an inhibition with Dox

La longévité

DEFF Research Database (Denmark)

Vaupel, James W.

2016-01-01

The rise of longevity since 1840 is remarkable. In France, life expectancy doubled from 40 in 1840 to 82 today. Analysis of the nature of this increase, including the improvements in survival at older ages, sheds new light on the economic, social and health transformations of the past two centuries...... are needed to anticipate coming economic, social and health changes....

Journal of Genetics | Indian Academy of Sciences

Indian Academy of Sciences (India)

Laboratório de História Natural de Anfíbios Brasileiros, Instituto de Biologia, Universidade Estadual de Campinas, 13083-862, Campinas, SP, Brazil; Departamento de Biologia, Universidade Estadual do Paraná, Campus União da Vitória, 84600-000, União da Vitória, PR, Brazil; Departamento de Zoologia, Universidade ...

Modern human sperm freezing: Effect on DNA, chromatin and acrosome integrity.

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Rahiminia, Tahereh; Hosseini, Akram; Anvari, Morteza; Ghasemi-Esmailabad, Saeed; Talebi, Ali Reza

2017-08-01

Presence of vitrification method in sperm freezing and the introduction of solid surface vitrification beside rapid freezing in vapour, opens an easy and safe way to help infertility centres. While the effects of cryopreservation on motility, morphology and viability of sperm are documented, the question of the probable alteration of sperm DNA, chromatin and acrosome integrity after freezing and thawing procedures in different methods is still controversial. Normal sample were collected according to WHO strict criteria. Sperm suspensions were mixed 1:1 with 0.5 M sucrose and divided into four equal aliquots for freezing: fresh, nitrogen direct immersion vitrification (Vit), solid surface vitrification (SSV) and in vapour (Vapour). Sperm suspensions were transferred into a 0.25 ml sterile plastic. Then straw was inserted inside the 0.5 ml straw. For thawing, the straws were immersed in a 42 °C water bath. Beside the sperm parameters, we assessed the acrosome reaction by double staining, chromatin integrity by toluidine blue (Tb) and chromomycin A3 (CMA3) and DNA integrity by terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) respectively. In progressive motility, the highest rate occurred in Vit (39.9 ± 13.3). Moreover, the lowest rate of immotile sperm was in Vit (32.7 ± 16.3). In normal morphology, the group Vit was similar to the fresh, while SSV and Vapour were significantly different from the fresh. The percentage of acrosome-reacted sperms was more in Vit (81.3 ± 10.2) than the fresh group. TUNEL+ results showed that DNA fragmentation was significantly increased in Vit (p-value = 0.025). While in SSV and Vapour results were comparable to fresh. There was a significant correlation between TUNEL+ and normal morphology, TB, CMA3 and presence of intact acrosome. Sperm in Vapour was healthier in terms of DNA, chromatin and acrosome integrity. In contrast of higher motility and normal morphology; DNA, chromatin and acrosome

Determination of potential role of antioxidative status and circulating biochemical markers in the pathogenesis of ethambutol induced toxic optic neuropathy among diabetic and non-diabetic patients.

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Rasool, Mahmood; Malik, Arif; Manan, Abdul; Aziz, Khuram; Mahmood, Amna; Zaheer, Saima; Shuja, Naveed; Qazi, Mahmood Husain; Kamal, Mohammad Amjad; Karim, Sajjad

2015-11-01

The present study was designed to explore the antioxidative status and circulating biochemical markers having a potential role in the pathogenesis of ethambutol (EMB) induced toxic optic neuropathy (TON) among diabetic and non-diabetic patients. Fifty patients under complete therapy of EMB for tuberculosis were included in the present study. Inclusion criteria for patients were to receive EMB everyday during treatment, a dose of 25 mg/kg for initial 2 months and 15 mg/kg during the rest of therapy period. We conducted color vision and visual acuity test for all patients. Fifteen out of fifty EMB induced TON patients, were found to be diabetic. Color vision and visual acuity test results were evaluated for diabetic and non-diabetic as well as twenty age matched controls. The results demonstrated a significant pattern of circulating biochemical markers between the studied groups. Data regarding hematological (RBC, p value = 0.02; Hemoglobin, p value = 0.02), hepatic (total bilirubin, p value = 0.01), renal (urea, p value = 0.03; creatinine, p value = 0.007), lipid (total cholesterol, p value = 0.01; total triglycerides, p value = 0.03) and antioxidative (superoxide dismutase, p value = 0.005; glutathione, p value = 0.02; catalase, p value = 0.02) profile showed a highly significant difference among the studied groups specially patients with diabetes. Malondialdehyde (MDA) level had gone significantly up in diabetic TON patients (p value = 0.02), in comparison to other antioxidants and vitamins (Vit). Vit-A, E, B1, B12 and Zinc seem to be playing a major role in the pathogenesis of TON, specially Vit-E and B1 surpassed all the antioxidants as having highly significant inverse relationships with MDA (MDA vs Vit-E, r = -0.676(**) and MDA vs Vit-B1, r = -0.724(**) respectively). We conclude that during the ethambutol therapy the decreased levels of Vit-E and Vit-B1 possibly play a role in the development of TON and may be used as therapeutic

Effect and mechanism of micronutrient compound on swimming endurance in mice with nutritional deficiency

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Jian-quan WU

2012-07-01

Full Text Available Objective To investigate the anti-fatigue effect and possible mechanisms of micronutrient compound. Methods One hundred and twenty-five male Kunming mice (SPF were fed following different feeding stuffs: sufficient feed, i.e. AIN-93M feed (corn starch 46.6%, casein 14%, dextrinized corn starch 15.5%, sucrose 10%, soybean oil 4%, fiber 5%, mineral mix 3.5%, vitamin mix 1%, L-cystine 0.18%, choline chloride 0.138% and tert-butylhydroquinone 8 ppm; insufficient feed (composed the same ingredients as the AIN-93M diet, replacing 30%, 50% or 70% of the content of mineral and vitamin mix with corn starch; compound micronutrient-added feed (CMAF, replacing 30% of the content of mineral and vitamin mix of AIN-93M diet with micronutrient compound composed of (g/kg Vit A 0.25, Vit B1 0.3, Vit B2 0.3, Vit B6 0.35, nicotinic acid 1.5, Vit D 0.05, Vit C 50, Vit E 10, calcium carbonate 180, ferrous glycine 1, zinc lactate 1 and corn starch 755.25. The mice were fed for 14 or 28 d, and then subjected to forced swim test with a load on their tails to exhaustion. The maximal swimming time was recorded. In another experiment, the Kunming mice were fed with corresponding feed for 28 d, and then subjected to forced swim test for 60 min, thereafter the samples of blood and liver were collected immediately. The serum contents of glucose, lactate, nonprotein nitrogen, nonesterified fatty acids and hepatic glycogen were measured. Results The dietary intake of 30% nutritional insufficient group (30% group was significantly decreased compared with normal control group (fed with AIN-93M diet, (P 0.05. The serum glucose and hepatic glycogen decreased and serum lactate, nonprotein nitrogen and nonesterified fatty acids increased in the control group, which were aggravated in nutrient insufficient group and reversed in micronutrient supplementation group. Conclusions Insufficient intake of micronutrients has significant influence on swimming endurance in mice and compound

Production, composition, and oxidative stability of milk highly enriched in polyunsaturated fatty acids from dairy cows fed alfalfa protein concentrate or supplemental vitamin E.

Science.gov (United States)

Fauteux, M-C; Gervais, R; Rico, D E; Lebeuf, Y; Chouinard, P Y

2016-06-01

Given its elevated content of carotenoids, alfalfa protein concentrates (APC) have the potential to prevent oxidation of milk enriched in polyunsaturated fatty acids. The effects of feeding APC or supplemental vitamin E on production, composition, and oxidative stability of milk enriched in polyunsaturated fatty acids were evaluated using 6 lactating Holstein cows (224±18d in milk) in a replicated 3×3 Latin square (21-d periods, 14d for adaptation). Treatment diets contained (dry matter basis) (1) 9% soybean meal (control, CTL); (2) 9% soybean meal + 300 IU of vitamin E/kg (VitE treatment); or (3) 9% APC (APC treatment). Cows received a continuous abomasal infusion of 450g/d of linseed oil. As a result, milk fat content of cis-9,cis-12 18:2 increased from 1.08±0.13 to 3.9±0.40% (mean ± SD), whereas cis-9,cis-12,cis-15 18:3 increased from 0.40±0.04 to 14.27±1.81% during the experimental period compared with the pretrial period. Milk yield tended to be higher for APC (14.7kg/d) compared with CTL (13.4kg/d), and was greater than that for VitE (13.0kg/d). Protein yield was higher in cows fed APC (518g/d) compared with VitE (445g/d) but was not different from that in cows fed CTL (483g/d). These effects resulted in improved milk N efficiency in cows fed APC (26.1% of N intake secreted in milk) compared with CTL (23.0%) and VitE (22.9%). Feeding APC increased milk fat content of lutein (252μg/g) compared with CTL (204μg/g) and VitE (190μg/g). Milk fat content of vitamin E was higher for APC (34.5μg/g) compared with CTL (19.0μg/g) and tended to be lower than that with VitE (44.9μg/g). Redox potential of fresh milk from cows fed APC (152mV) was similar to that of VitE (144mV), but lower than that of CTL (189mV). Treatments had no effect on fresh milk contents of dissolved oxygen (8.1±1.5mg/L), and conjugated diene hydroperoxides (2.7±0.5mmol/L). The concentrations of volatile lipid oxidation products (propanal, hexanal, hept-cis-4-enal, 1-octen-3-one) tended

Vitamin D-biofortified beef

DEFF Research Database (Denmark)

Duffy, Sarah K.; O'Doherty, John V.; Rajauria, Gaurav

2018-01-01

This study investigates dietary fortification of heifer feeds with cholecalciferol and ergocalciferol sources and effects on beef total vitamin D activity, vitamer, respective 25-hydroxymetabolite contents, and meat quality. Thirty heifers were allocated to one of three dietary treatments [(1......) basal diet + 4000 IU of vitamin D3 (Vit D3); (2) basal diet + 4000 IU of vitamin D2 (Vit D2); and (3) basal diet + 4000 IU of vitamin D2-enriched mushrooms (Mushroom D2)] for a 30 day pre-slaughter period. Supplementation of heifer diets with Vit D3 yielded higher (p ...) total vitamin D activity (by 38–56%; p vitamin D source, carcass characteristics, sensory and meat quality parameter were unaffected (p > 0.05) by the dietary...

Vacuum-induced quantum memory in an opto-electromechanical system

Science.gov (United States)

Qin, Li-Guo; Wang, Zhong-Yang; Wu, Shi-Chao; Gong, Shang-Qing; Ma, Hong-Yang; Jing, Jun

2018-03-01

We propose a scheme to implement electrically controlled quantum memory based on vacuum-induced transparency (VIT) in a high-Q tunable cavity, which is capacitively coupled to a mechanically variable capacitor by a charged mechanical cavity mirror as an interface. We analyze the changes of the cavity photons arising from vacuum-induced-Raman process and discuss VIT in an atomic ensemble trapped in the cavity. By slowly adjusting the voltage on the capacitor, the VIT can be adiabatically switched on or off, meanwhile, the transfer between the probe photon state and the atomic spin state can be electrically and adiabatically modulated. Therefore, we demonstrate a vacuum-induced quantum memory by electrically manipulating the mechanical mirror of the cavity based on electromagnetically induced transparency mechanism.

Effect of selenium-vitamin E injection in selenium-deficient dairy goats and kids on the Mexican plateau Efeito do selênio e da vitamina E sobre a produtividade de cabras e cabritos no México

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J.E. Ramírez-Bribiesca

2005-02-01

Full Text Available Three experiments were carried out to determine the optimum selenium-vitamin E injection level to maintain acceptable blood selenium (Se status of does and kids, as well as to determine the relation of that status to mortality rates in kids. In experiment 1, 238 goats were assigned to one of three groups during the mating period: A1-control, A2- 0.06mgSe+0.8IU vitE/kgBW and A3-0.125mgSe+1.7IU vitE/kgBW. No differences (P>.05 for fertility and prolificacy were observed among the groups. Blood Se concentration did not differ among Se-vit E groups and control group before injection, and both groups showed Se deficient condition. There was a trend (PForam conduzidos três experimentos para determinar a quantidade ótima da injeção de selênio-vitamina E (Se-vit E para manter o nível aceitável do status de selênio no sangue de cabras e cabritos e determinar essa relação com o status sobre a taxa de mortalidade de cabritos. No primeiro experimento, 238 cabras foram usados em um dos três grupos durante o período do acasalamento: A1- controle, A2- 0,06mgSe+0,8UI vit E/kg de peso-vivo e A3- 0,125mgSe+1,7UI vitE/kg de peso-vivo. Não houve diferenças (P>0,05 entre os grupos para fertilidade e prolificidade. A concentração de Se no sangue não foi diferente entre os grupos que receberam Se-vit E e o controle antes da injeção e ambos os grupos mostravam deficiência de Se. Sessenta dias pós-tratamento houve tendência (P<0,05; 32% de aumentar o nível de Se no sangue e não houve diferença entre os grupos A2 e controle. Houve diferença significativa (P<0,05; 103% entre os grupos A1 e A2 e o grupo A3. No segundo experimento, 48 cabras foram divididas em quatro grupos: B1- controle, B2- 0,125mgSe+1,7UI vitE/kg de peso-vivo, B3- 0,25mgSe+3,4UI vitE/kg de peso-vivo e B4- 0,31mgSe+4,2UI vitE/kg de peso-vivo. O grupo B4 alcançou a concentração mais alta no terceiro mês depois da injeção (0,11ppm e diminuiu depois de 100 dias, quando o valor

Resolução de um caso real do problema dial-a-ride multicritério via clustering search

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Patrícia Perretto Rodrigues

2014-09-01

Full Text Available Este artigo apresenta um clustering search (CS para o problema dial-a-ride (DARP multicritério presente na cidade de Vitória (ES. Em Vitória, os usuários especificam requisições de transporte entre origens e destinos com janelas de tempo para os horários de embarque e desembarque; o transporte é realizado por uma frota homogênea de veículos localizados inicialmente em uma mesma garagem; busca-se assim definir um conjunto de rotas de atendimento que minimize o custo de transporte (normalmente, tempo ou distância, o número de veículos utilizados e o tempo total de espera dos usuários, respeitando restrições como as de capacidade dos veículos e de precedência (o embarque de um usuário deve preceder o seu destino em uma rota. Dessa forma, o CS aqui proposto foi capaz de tratar as particularidades do caso de Vitória, ES. Bons resultados computacionais são apresentados considerando instâncias reais obtidas na Secretaria de Transportes, Trânsito e Infraestrutura Urbana de Vitória, ES.

The role of vitamin E in the prevention of zoledronic acid-induced nephrotoxicity in rats: a light and electron microscopy study.

Science.gov (United States)

Sert, İbrahim Unal; Kilic, Ozcan; Akand, Murat; Saglik, Lutfi; Avunduk, Mustafa Cihat; Erdemli, Esra

2018-03-01

Bisphosphonates are widely used in metastatic cancer such as prostate and breast cancer, and their nephrotoxic effects have been established previously. In this study we aimed to evaluate both the nephrotoxic effects of zoledronic acid (ZA) and the protective effects of vitamin E (Vit-E) on this process under light and electron microscopy. A total of 30 male Sprague-Dawley rats were divided into 3 groups. The first group constituted the control group. The second group was given i.v. ZA of 3 mg/kg once every 3 weeks for 12 weeks from the tail vein. The third group received the same dosage of ZA with an additional i.m . injection of 15 mg Vit-E every week for 12 weeks. Tissues were taken 4 days after the last dose of ZA for histopathological and ultrastructural evaluation. Paller score, tubular epithelial thickness and basal membrane thickness were calculated for each group. For group 2, the p -values are all < 0.001 for Paller score, epitelial thickness, and basal membrane thickness. For group 3 (ZA + Vit. E), the p -values are < 0.001 for Paller score, 0.996 for epitelial thickness, and < 0.001 basal membrane thickness. Significant differences were also observed in ultrastructural changes for group 2. However, adding Vit-E to ZA administration reversed all the histopathological changes to some degree, with statistical significance. Administration of ZA had nephrotoxic effects on rat kidney observed under both light and electron microscopy. Concomitant administration of Vit-E significantly reduces toxic histopathological effects of ZA.

Arsenic Induced Toxicity in Broiler Chicks and Its Amelioration with Ascorbic Acid: Clinical, Hematological and Pathological Study

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Rabia Sharaf, Ahrar Khan*, Muhammad Zargham Khan, Iftikhar Hussain, Rao Zahid Abbas, S. T. Gul, Fazal Mahmood and Muhammad Kashif Saleemi

2013-07-01

Full Text Available This study was conducted to observe the arsenic (As toxicity lesions in birds and to know either Vit C ameliorates these toxic effects or not. One-day-old broilers chicks (n=72 procured from a local hatchery were randomly divided into four equal groups. First group was kept as control and second group was given As (50 mg/kg BW via crop tubing. Third group received in addition to As, Vit C (250 mg/kg BW whereas fourth group received only Vit C. Killing by neck dislocation of randomly selected six birds from each group was carried out on experimental days 0, 16 and 32 for collection of blood and tissues specimens. Arsenic treated birds showed clinical signs of toxicity throughout the experiment than all other groups. These clinical signs included decreased body weight and feed intake, dullness, open mouth breathing, increased thirst, ruffled feathers, pale comb, skin irritation and watery diarrhea which were not significant in any other group. As treated group showed a significant (P<0.05 decrease in hematological parameters. Severe gross and histopathological changes were observed in intestines, spleen and lungs of birds fed with As than all other groups. Decreased height of villi of middle portion of small intestines was also observed in As treated birds. Villi height in Vit C treated group increased as compared to control group. It was concluded that As induces severe toxic effects in broiler birds; however, these toxic effects can be partially ameliorated by Vit C.

Calcium, zinc and vitamin E ameliorate cadmium-induced renal oxidative damage in albino Wistar rats

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Pradeepkiran Jangampalli Adi

Full Text Available This study was aimed to examine the protective effects of supplementation with calcium + zinc (Ca + Zn or vitamin E (Vit-E on Cd-induced renal oxidative damage. Young albino Wistar rats (180 ± 10 g (n = 6 control rats, Cd, Cd + Ca + Zn, and Cd + Vit-E experimental groups and the experimental period was 30 days. Rats were exposed to Cd (20 mg/kg body weight alone treated as Cd treated group and the absence or presence of Ca + Zn (2 mg/kg each or Vit-E (20 mg/kg body weight supplementation treated as two separate groups. The activities of the stress marker enzymes superoxide dismutase (SOD, catalase (CAT, glutathione reductase (GR, glutathione peroxidase (GPx, glutathione-S-transferase (GST and lipid peroxidase (LPx were determined in renal mitochondrial fractions of experimental rats. We observed quantitative changes in SOD isoenzymatic patterns by non-denaturing PAGE analysis, and quantified band densities. These results showed that Cd exposure leads to decreases in SOD, CAT, GR, and GPx activities and a concomitant increase in LPx and GST activities. Ca + Zn and Vit-E administration with Cd significantly reversed Cd-induced perturbations in oxidative stress marker enzymes. However, Vit-E showed more inhibitory activity against Cd than did Ca + Zn, and it protected against Cd-induced nephrotoxicity. Keywords: Cadmium (Cd, Oxidative stress, Lipid peroxidation, Nephrotoxicity, PAGE analysis

Vitamin D2 Supplementation Amplifies Eccentric Exercise-Induced Muscle Damage in NASCAR Pit Crew Athletes

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David C. Nieman

2013-12-01

Full Text Available This study determined if 6-weeks vitamin D2 supplementation (vitD2, 3800 IU/day had an influence on muscle function, eccentric exercise-induced muscle damage (EIMD, and delayed onset of muscle soreness (DOMS in National Association for Stock Car Auto Racing (NASCAR NASCAR pit crew athletes. Subjects were randomized to vitD2 (n = 13 and placebo (n = 15, and ingested supplements (double-blind for six weeks. Blood samples were collected and muscle function tests conducted pre- and post-study (leg-back and hand grip dynamometer strength tests, body weight bench press to exhaustion, vertical jump, 30-s Wingate test. Post-study, subjects engaged in 90 min eccentric-based exercise, with blood samples and DOMS ratings obtained immediately after and 1- and 2-days post-exercise. Six weeks vitD2 increased serum 25(OHD2 456% and decreased 25(OHD3 21% versus placebo (p < 0.001, p = 0.036, respectively, with no influence on muscle function test scores. The post-study eccentric exercise bout induced EIMD and DOMS, with higher muscle damage biomarkers measured in vitD2 compared to placebo (myoglobin 252%, 122% increase, respectively, p = 0.001; creatine phosphokinase 24 h post-exercise, 169%, 32%, p < 0.001, with no differences for DOMS. In summary, 6-weeks vitD2 (3800 IU/day significantly increased 25(OHD2 and decreased 25(OHD3, had no effect on muscle function tests, and amplified muscle damage markers in NASCAR pit crew athletes following eccentric exercise.

Intrinsic factors rather than vitamin D deficiency are related to insulin resistance in lean women with polycystic ovary syndrome.

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Sahin, S; Eroglu, M; Selcuk, S; Turkgeldi, L; Kozali, S; Davutoglu, S; Muhcu, M

2014-10-01

To investigate the correlation between insulin resistance (IR) and serum 25-OH-Vit D concentrations and hormonal parameters in lean women with polycystic ovary syndrome (PCOS). 50 lean women with PCOS and 40 body mass index (BMI) matched controls were compared in terms of fasting insulin and glucose, homeostatic model assessment insulin resistance (HOMA-IR), 25-OH-Vit D, high sensitivity C-reactive protein (hs-CRP), luteinizing hormone (LH), follicle-stimulating hormone (FSH), total testosterone, dehydroepiandrosterone sulfate (DHEA-S), total cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL), triglycerides and Ferriman-Gallway (FG) scores. Correlation analyses were performed between HOMA-IR and metabolic and endocrine parameters. 30% of patients with PCOS demonstrated IR. Levels of 25-OH-Vit D, hsCRP, cholesterol, HDL, LDL, triglyceride and fasting glucose did not differ between the study and control groups. Fasting insulin, HOMA-IR, LH, total testosterone, and DHEA-S levels were higher in PCOS group. HOMA-IR was found to correlate with hs-CRP and total testosterone but not with 25-OH-Vit D levels in lean patients with PCOS. An association between 25-OH-Vit D levels and IR is not evident in lean women with PCOS. hs-CRP levels do not indicate to an increased risk of cardiovascular disease in this population of patients. Because a strong association between hyperinsulinemia and hyperandrogenism exists in lean women with PCOS, it is advisable for this population of patients to be screened for metabolic disturbances, especially in whom chronic anovulation and hyperandrogenism are observed together.

Influence of altitude on vitamin D and bone metabolism of lactating sheep and goats.

Science.gov (United States)

Kohler, M; Leiber, F; Willems, H; Merbold, L; Liesegang, A

2013-11-01

This study investigated the influence of alpine grazing on vitamin D (vitD) and bone metabolism in sheep and goats. Two groups of five adult lactating East Friesian milk sheep and Saanen dairy goats were kept on pastures at 2,000 to 2,600 m a.s.l. (SA: sheep alpine; GA: goats alpine) and 400 m a.s.l. (SL: sheep lowland; GL: goats lowland). The animals were milked twice daily and the milk yield was measured. Blood, milk, skin, and forage samples were collected and the left metatarsi were measured with peripheral quantitative computed tomography. The relative humidity and air temperature were recorded and the ultraviolet B (UVB) radiation was measured with a solar meter at both research stations. In addition, animals from the alpine group were equipped with a global positioning system receiver. The UVB radiation was higher at the alpine station (Psheep compared to goats and the 25(OH)D3 concentration in serum increased in all four groups but was higher in the alpine groups during the experiment. In addition, no differences in 7-dehydrocholesterol (7-DHC) concentrations in the skin at high altitude and lowland groups were detectable. However the 7-DHC concentrations in the skin of sheep were less than a tenth of the concentrations in the skin of goats and were nearly not detectable. In both groups SA and SL bone strength index increased during the trial (P=0.043). Bone strength index was lower in GA compared to GL at wk 12 (P=0.047). Mean serum Ca concentrations were higher and P concentrations were lower in the alpine groups than in the lowland groups (P=0.047). In both groups SA and GA the distance travelled increased during the trial. In conclusion, no effect of altitude on vitD status, vitD milk concentration and bone strength could be detected. Both sheep and goats are able to produce vitD in the skin, but sheep depend more on vitD intake with feedstuff, whereas goats rely more on cutaneous vitD production.

Vitamin D insufficiency reduces the protective effect of bisphosphonate on ovariectomy-induced bone loss in rats.

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Mastaglia, Silvina R; Pellegrini, Gretel G; Mandalunis, Patricia M; Gonzales Chaves, Macarena M; Friedman, Silvia M; Zeni, Susana N

2006-10-01

The present study was carried out to obtain an experimental model of vitamin D (vit D) insufficiency and established osteopenia (experiment 1) to then investigate whether vit D status, i.e. normal or insufficient, interferes with bone mass recovery resulting from bisphosphonate therapy (experiment 2). Rats (n = 40) underwent OVX (n = 32) or a sham operation (n = 8). The first 15 days post-surgery, all groups were kept under fluorescent tube lighting and fed a diet containing 200 IU% vit D (+D). They were then assigned during an additional 45 days to receive either +D or a diet lacking vit D (-D) and kept under 12 h light/dark cycles using fluorescent or red lighting. Serum 25HOD was significantly lower in -D rats (P < 0.0001). The type of lighting did not induce differences in 25OHD, calcium (sCa), phosphorus (sP), bone alkaline phosphatase (b-AL), CTX, bone density or histology. No osteoid was observed in undecalcified bone sections. Experiment 2 (105 days): rats were fed either +D or -D according to experiment 1 and were treated with either placebo or 16 mug olpadronate (OPD)/100 g rat/week during the last 45 days. Whereas 25HOD was significantly lower (P < 0.0001) in -D/OPD than in +D/OPD rats, no significant differences in sCa, sP, b-AL or CTX were observed. OPD prevented the loss of lumbar spine (LS) and proximal tibia (PT) BMD and the decrease in bone volume (BV/TV) (P < 0.05) and in the number of trabeculae observed in untreated rats. However, +D/OPD animals presented significantly higher values of LS BMD, PT BMD and BV/TV than -D/OPD rats (P < 0.05). No osteoid was observed in undecalcified sections of bone. In summary, this is the first experimental study to provide evidence that differences in vit D status may affect the anticatabolic response to bisphosphonate treatment. However, the molecular mechanism through which vit D insufficiency reduces the effect of the aminobisphosphonate remains to be defined.

Índice de área foliar, spad e massa de matéria seca do feijoeiro comum em função dos bioestimulantes, NPK e micronutrientes em Vitória da Conquista – BA

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Danilo Nogueira dos Anjos

2017-04-01

Full Text Available A cultura do feijão apresenta grande importância econômica e social no Brasil e estudos sobre a função dos bioestimulantes associados a diferentes adubações nessa cultura ainda são incipientes. Desse modo, objetivou-se com este trabalho avaliar a influência do uso de bioestimulantes e suas interações com as adubações de NPK e micronutrientes no índice SPAD, índice de área foliar e a massa de matéria seca do feijoeiro da cultivar de feijão Pérola no município de Vitória da Conquista - BA. O trabalho foi desenvolvido na Universidade Estadual do Sudoeste da Bahia e teve o delineamento experimental em blocos casualizados arranjados em três fatores 2x2x4 sendo dois níveis de NPK (ausência e presença, dois níveis de micronutrientes (ausência e presença e os bioestimulantes (ausência, Stimulate, Booster e Biozyme TF. Houve reposta significativa entre a interação dos bioestimulantes e NPK para o índice de área foliar e massa seca da parte aérea, com destaque para os bioestimulantes Booster e Biozyme TF que na ausência do NPK aumentaram o índice de área foliar. O NPK incrementou a massa seca da haste e o índice SPAD do feijoeiro.

Vertical interincisal trespass assessment in children with speech disorders

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Marcelo de Gouveia Sahad

2008-09-01

Full Text Available Through a transversal epidemiological study, conducted with 333 Brazilian children, males (157 and females (176, aged 3 to 6 years old, enrolled in a public preschool, this study aimed to evaluate the prevalence of the different types of vertical interincisal trespass (VIT and the relationship between these occlusal aspects and anterior lisping and/or anterior tongue thrust in the articulation of the lingua-alveolar phonemes /t/, /d/, /n/ and /l/. All children involved were submitted to a VIT examination and to a speech evaluation. Statistical significance was analyzed through the Qui-square test, at a significance level of 0.05 (95% confidence limit. The quantitative analysis of the data demonstrated the following prevalences: 1 - the different types of VIT: 48.3% for normal overbite (NO, 22.5% for deep overbite (DO, 9.3% for edge to edge (ETE and 19.8% for open bite (OB; 2 - interdental lisping in relation to the different types of VIT: 42% for NO, 12.5% for DO, 12.5% for ETE, 32.9% for OB; and 3 - children with anterior tongue thrust in the articulation of lingua-alveolar phonemes in relation to the different types of VIT: 42.1% for NO, 14% for DO, 10.5% for ETE, 33.3% for OB. The results demonstrated that there was a significant relationship between open bite and anterior lisping and/or anterior tongue thrust in the articulation of the lingua-alveolar phonemes /t/, /d/, /n/ and /l/; and that there was a significant relationship between deep overbite and the absence of anterior lisping and anterior tongue thrust in the articulation of the lingua-alveolar phonemes.

Which immunotherapy product is better for patients allergic to Polistes venom? A laboratory and clinical study.

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Eleonora Savi

Full Text Available Venom immunotherapy (VIT is highly effective in preventing allergic reactions to insect stings, but the appropriate venom must be used to achieve clinical protection. In patients with multiple positive results to venoms, molecular allergy diagnostics or CAP-inhibition may identify the causative venom. Concerning allergy to venom from Polistes spp. it has been proposed that only the European species P. dominulus should be used for VIT. However, this recommendation is not present in any international guideline. Using both laboratory and clinical data, we aimed to evaluate the reliability of this proposal.We performed an in vitro study using CAP-inhibition to determine sensitization of 19 patients allergic to Polistes venom. The clinical study included 191 patients with positive tests to Polistes treated with VIT, 102 were treated with P. dominulus and 89 were treated with a mix of American Polistes (mAP.The difference in % of inhibition was significant concerning inhibition of P. dominulus sIgE by P. dominulus venom (79.8% compared with inhibition by mAP venom (64.2% and not significant concerning the inhibition of mAP sIgE by P. dominulus venom (80.1% and by mAP venom (73.6%. Instead, the clinical protection from stings was not statistically different between the two kinds of venom.The data from CAP inhibition would suggest that the choice of either P. dominulus venom or mAP venom for VIT is appropriate in patients with CAP inhibition higher than 70%, but the clinical data show the same odds of protection from stings using for VIT P. dominulus or mAP venom.

Which immunotherapy product is better for patients allergic to Polistes venom? A laboratory and clinical study.

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Savi, Eleonora; Incorvaia, Cristoforo; Boni, Elisa; Mauro, Marina; Peveri, Silvia; Pravettoni, Valerio; Quercia, Oliviero; Reccardini, Federico; Montagni, Marcello; Pessina, Laura; Ridolo, Erminia

2017-01-01

Venom immunotherapy (VIT) is highly effective in preventing allergic reactions to insect stings, but the appropriate venom must be used to achieve clinical protection. In patients with multiple positive results to venoms, molecular allergy diagnostics or CAP-inhibition may identify the causative venom. Concerning allergy to venom from Polistes spp. it has been proposed that only the European species P. dominulus should be used for VIT. However, this recommendation is not present in any international guideline. Using both laboratory and clinical data, we aimed to evaluate the reliability of this proposal. We performed an in vitro study using CAP-inhibition to determine sensitization of 19 patients allergic to Polistes venom. The clinical study included 191 patients with positive tests to Polistes treated with VIT, 102 were treated with P. dominulus and 89 were treated with a mix of American Polistes (mAP). The difference in % of inhibition was significant concerning inhibition of P. dominulus sIgE by P. dominulus venom (79.8%) compared with inhibition by mAP venom (64.2%) and not significant concerning the inhibition of mAP sIgE by P. dominulus venom (80.1%) and by mAP venom (73.6%). Instead, the clinical protection from stings was not statistically different between the two kinds of venom. The data from CAP inhibition would suggest that the choice of either P. dominulus venom or mAP venom for VIT is appropriate in patients with CAP inhibition higher than 70%, but the clinical data show the same odds of protection from stings using for VIT P. dominulus or mAP venom.

Alternative expression of vacuolar iron transporter and ferritin genes leads to blue/purple coloration of flowers in tulip cv. 'Murasakizuisho'.

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Shoji, Kazuaki; Momonoi, Kazumi; Tsuji, Tosiaki

2010-02-01

Flowers of tulip cv. 'Murasakizuisho' have a purple perianth except for the bottom region, which is blue in color even though it has the same anthocyanin, delphinidin 3-O-rutinoside, as the entire perianth. The development of the blue coloration in the perianth bottom is due to complexation by anthocyanin, flavonol and iron (Fe), as well as a vacuolar iron transporter, TgVit1. Although transient expression of TgVit1 in the purple cells led to a color change to light blue, the coloration of the transformed cells did not coincide with the dark blue color of the cells of the perianth bottom. We thought that another factor is required for the blue coloration of the cells of perianth bottom. To examine the effect of ferritin (FER), an Fe storage protein, on blue color development, we cloned an FER gene (TgFER1) and performed expression analyses. TgFER1 transcripts were found in the cells located in the upper region of the petals along with purple color development by anthocyanin and were not found in the blue cells of the perianth bottom. This gene expression is in contrast to that of TgVit1, expressed only in the cells of the perianth bottom. Co-expression of TgVIT1 and TgFER-RNAi, constructed for suppressing endogenous TgFER1 by RNA interference (RNAi), changed the purple petal cells to a dark blue color similar to that of the natural perianth bottom. These results strongly suggest that TgVit1 expression and TgFER1 suppression are critical for the development of blue color in the perianth bottom.

Antioxidant effect of vitamin E and 5-aminosalicylic acid on acrylamide induced kidney injury in rats

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Nisreen A. Rajeh

2017-02-01

Full Text Available exposure of acrylamide and to study the protective effect of 5-Aminosalicylic acid (5-ASA and Vitamin E (vit-Eon Acrylamide (ACR induced renal toxicity. Methods: This study was conducted at King Fahad Medical Research Centre, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia, between August and November 2015. A total of 49 adult Wistar rats (250 ± 20g aged 60 days were kept in a controlled environment and used in the present study. The rats were divided into 7 groups (control, ACR alone, ACR+5-ASA, ACR+vit-E, ACR+ASA+vit-E, vit-E alone, and ASA alone. After 5 days of ACR oral gavage treatment, the rats were observed for 24 hours then killed. Histopathology for the kidney and lactate dehydrogenase assay were carried out. Results: Acrylamide produced significant pathological changes in the kidney with acute tubular necrosis in the distal tubules that could be reversed by concomitant injection of rat with 5-ASA. Together with vitamin E, 5-ASA, showed maximum renal protection. No statistically significant difference was observed in either body weights or lactate dehydrogenase activity of ACR treated rats. Conclusion: Acrylamide exposure leads to adverse clinical pathologies of renal tubules, which were reversed by a concomitant treatment with 5-ASA and vitamin-E

AC Calorimetry and Thermophysical Properties of Bulk Glass-Forming Metallic Liquids

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Johnson, William L.

2000-01-01

Thermo-physical properties of two bulk metallic glass forming alloys, Ti34Zr11Cu47Ni8 (VIT 101) and Zr57Nb5Ni12.6Al10CU15.4 (VIT 106), were investigated in the stable and undercooled melt. Our investigation focused on measurements of the specific heat in the stable and undercooled liquid using the method of AC modulation calorimetry. The VIT 106 exhibited a maximum undercooling of 140 K in free radiative cooling. Specific heat measurements could be performed in stable melt down to an undercooling of 80 K. Analysis of the specific heat data indicate an anomaly near the equilibrium liquidus temperature. This anomaly is also observed in y the temperature dependencies of the external relaxation time, the specific volume, and the surface tension; it is tentatively attributed to a phase separation in the liquid state. The VIT 101 specimen exhibited a small undercooling of about 50 K. Specific heat measurements were performed in the stable and undercooled melt. These various results will be combined with ground based work such as the measurement of T-T-T curves in the electrostatic levitator and low temperature viscosity and specific heat measurements for modeling the nucleation kinetics of these alloys.

The Ability of Bile to Scavenge Superoxide Radicals and Pigment Gallstone Formation in Guinea Pigs

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Cong Lin

1996-01-01

Full Text Available After partial ligation of the common bile duct (CBD of guinea pigs, 14 of 16 animals developed pigment gallstones within one week (S group. Intraperitoneal injection of Vit. E and C, each 10 mg/kg daily from 3 days before CBD ligation to one week after the operation (S+V group, decreased the gallstone incidence to 5/14 (exact probability0.05, but Vit. E and C normalized the SR, and the difference between S group and S+V group was significant (p<0.05. These results suggested that Vit. E and C, known as antioxidants, enhanced the ability to scavenge oxygen radical in S+V group; and that in addition to the increases of UCB and Ca2+ concentrations, the participation of oxygen radicals might be of importance for pigment gallstone formation induced by bile duct obstruction.

A combination of ascorbic acid and α-tocopherol or a combination of Mg and Zn are both able to reduce the adverse effects of lindane-poisoning on rat brain and liver.

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Hfaiedh, Najla; Murat, Jean-Claude; Elfeki, Abdelfettah

2012-10-01

The purpose of this study, carried out on male Wistar rats, was to evaluate the beneficial effects of supplementation with ascorbic acid (Vit C) and α-tocopherol (Vit E) or with Mg and Zn upon lindane-induced damages in liver and brain. Under our experimental conditions, lindane poisoning (5mg/kg body weight per day for 3 days) resulted in (1) an increased level of plasma glucose, cholesterol and triglycerides, (2) an increased activity of LDH, ALP, AST, ALT, (3) an oxidative stress in liver and brain as revealed by an increased level of lipids peroxidation (TBARS) and a decrease of glutathione-peroxidase, superoxide dismutase and catalase activities in liver and brain. In conclusion, both Vit C+E or Mg+Zn treatments display beneficial effects upon oxidative stress induced by lindane treatment in liver and brain. Copyright © 2012 Elsevier GmbH. All rights reserved.

L-Ascorbate Protects Against Methamphetamine-Induced Neurotoxicity of Cortical Cells via Inhibiting Oxidative Stress, Autophagy, and Apoptosis.

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Huang, Ya-Ni; Yang, Ling-Yu; Wang, Jing-Ya; Lai, Chien-Cheng; Chiu, Chien-Tsai; Wang, Jia-Yi

2017-01-01

Methamphetamine (METH)-induced cell death contributes to the pathogenesis of neurotoxicity; however, the relative roles of oxidative stress, apoptosis, and autophagy remain unclear. L-Ascorbate, also called vitamin (Vit.) C, confers partial protection against METH neurotoxicity via induction of heme oxygenase-1. We further investigated the role of Vit. C in METH-induced oxidative stress, apoptosis, and autophagy in cortical cells. Exposure to lower concentrations (0.1, 0.5, 1 mM) of METH had insignificant effects on ROS production, whereas cells exposed to 5 mM METH exhibited ROS production in a time-dependent manner. We confirmed METH-induced apoptosis (by nuclear morphology revealed by Hoechst 33258 staining and Western blot showing the protein levels of pro-caspase 3 and cleaved caspase 3) and autophagy (by Western blot showing the protein levels of Belin-1 and conversion of microtubule-associated light chain (LC)3-I to LC3-II and autophagosome staining by monodansylcadaverine). The apoptosis as revealed by cleaved caspase-3 expression marked an increase at 18 h after METH exposure while both autophagic markers, Beclin 1 and LC3-II, marked an increase in cells exposed to METH for 6 and 24 h, respectively. Treating cells with Vit. C 30 min before METH exposure time-dependently attenuated the production of ROS. Vitamin C also attenuated METH-induced Beclin 1 and LC3-II expression and METH toxicity. Treatment of cells with Vit. C before METH exposure attenuated the expression of cleaved caspase-3 and reduced the number of METH-induced apoptotic cells. We suggest that the protective effect of Vit. C against METH toxicity might be through attenuation of ROS production, autophagy, and apoptosis.

A comparison of the effects of Portulaca oleracea seeds hydro-alcoholic extract and Vitamin C on biochemical, hemodynamic and functional parameters in cardiac tissue of rats with subclinical hyperthyroidism

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Khodadadi, Hadi; Pakdel, Roghayeh; Khazaei, Majid; Niazmand, Said; Bavarsad, Kowsar; Hadjzadeh, Mousa AL-Reza

2018-01-01

Objective: The present study was performed to evaluate the effects of hydro-alcoholic extract of Portulaca oleracea (P. oleracea) seeds and Vitamin C on biochemical and hemodynamic parameters in cardiac tissue of rats with subclinical hyperthyroidism. Materials and Methods: Forty eight male rats were divided into six groups of 8 and treated for 4 weeks. T4 group received daily injection of levothyroxine sodium (20 μg/kg) and control group was given daily injection of saline. T4-Po groups were given T4 plus 100, 200, and 400 mg/kg of P. oleracea seeds extract in drinking water daily. T4-Vit C group received T4 plus daily injection of Vitamin C (100 mg/kg). At the end of the experiment, body weight, serum free T4 level, left ventricular developed pressure (LVDP), malondialdehyde (MDA) and total thiol levels were measured. Results: Free T4 levels were increased in all groups that were treated with T4. Weight gain was decreased in T4 and T4-Po100 groups compared to control group (p<0.001 and p<0.05). However, body weight was increased in T4-Po (200 and 400) and T4-Vit C groups compared to T4 group. LVDP was increased in T4 group compared to control group but, LVDP was decreased in T4-Po and T4-Vit C groups. Malondialdehyde was decreased in T4-Po groups and T4-Vit C group compared to T4 group. Total thiol groups were increased in T4-Po (200 and 400) and T4-Vit C groups compared to T4 group. Conclusion: The results showed that P. oleracea extract has a protective effect on cardiac dysfunction due to subclinical hyperthyroidism induced by levothyroxine sodium in rats. PMID:29632847

Long-Term Follow-Up of Children after Venom Immunotherapy: Low Adherence to Anaphylaxis Guidelines.

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Fiedler, Claudia; Miehe, Ulrich; Treudler, Regina; Kiess, Wieland; Prenzel, Freerk

2017-01-01

Data on the long-term outcome of children after specific venom immunotherapy (VIT) are limited. Therefore, we assessed sting recurrence and anaphylaxis relapse rates as well as adherence to anaphylaxis guidelines with regard to the availability of emergency equipment and education status. For this long-term survey, data of 311 children with a history of anaphylactic reactions to hymenoptera stings were collected by chart review. We included patients who were treated with a 3-year VIT between 1993 and 2009 and had completed a questionnaire. Forty of the 311 patients were included. Mean VIT duration was 3.1 years. Of the 40 patients included, 29 children (72.5%) received VIT with vespid venom, 9 with bee venom, and 2 patients with both venoms. During a mean follow-up period of 13 years, 20/40 patients (50%) suffered re-stings. Six of the 20 (30%) patients developed again anaphylactic symptoms (grade 1 n = 5, grade 3 n = 1); 2 were allergic to vespid and 4 to bee venom. Of the entire cohort, only 5/40 (12.5%) had appropriate emergency kits according to the guidelines of the European Academy of Allergy and Clinical Immunology. Among the patients who had emergency kits available, one third (5/15) felt uncertain about the correct application of the medication. Less than two thirds of our population (25/40) affirmed that they have been educated in emergency management. The vast majority (95%; 38/40) of our patients did not have allergy follow-ups after VIT completion. Anaphylactic relapses are not uncommon, and there are considerable deficits in the emergency management of patients. Hence, comprehensive standardized anaphylaxis education programs as well as regular follow-ups of the allergy status are crucial. © 2017 S. Karger AG, Basel.

Potential Cardiovascular and Renal Protective Effects of Vitamin D and Coenzyme Q10 in l-NAME-Induced Hypertensive Rats.

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Shamardl, Hanan A; El-Ashmony, Sahar M; Kamel, Hala F; Fatani, Sameer H

2017-08-01

Hypertension is one of the primary modifiable risk factors for cardiovascular disease. Adequate vitamin D (vit D) levels have been shown to reduce vascular smooth muscle contraction and to increase arterial compliance, which may be beneficial in hypertension. Further, coenzyme Q10 (COQ10) through its action to lower oxidative stress has been reported to have beneficial effects on hypertension and heart failure. This study examined the possible cardiac and renal protective effects of vit D and COQ10 both separately and in combination with an angiotensin II receptor blocker, valsartan (vals) in l-NAME hypertensive rats. Hypertension was induced in rats by l-NAME administration. Following induction of hypertension, the rats were assigned into the following 6 subgroups: an l-NAME alone group and treated groups receiving the following drugs intraperitoneally for 6 weeks; vals, vit D, COQ10 and combination of vals with either vit D or COQ10. A group of normotensive rats were used as negative controls. At the end of the treatment period, blood pressure, serum creatinine, blood urea nitrogen, lipids and serum, cardiac and renal parameters of oxidative stress were measured. Compared to the l-NAME only group, all treatments lowered systolic, diastolic, mean arterial pressure, total cholesterol, low-density lipoprotein cholesterol, and creatinine levels as well as TNF-α and malondialdehyde. Further, the agents increased serum, cardiac and renal total antioxidant capacity. Interestingly, the combination of agents had further effects on all the parameters compared to treatment with each single agent. The study suggests that the additive protective effects of vit D and COQ10 when used alone or concurrent with vals treatment in hypertensive rats may be due to their effects as antioxidants, anticytokines and blood pressure conservers. Copyright © 2017 Southern Society for Clinical Investigation. Published by Elsevier Inc. All rights reserved.

Allergen immunotherapy for insect venom allergy: a systematic review and meta-analysis.

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Dhami, S; Zaman, H; Varga, E-M; Sturm, G J; Muraro, A; Akdis, C A; Antolín-Amérigo, D; Bilò, M B; Bokanovic, D; Calderon, M A; Cichocka-Jarosz, E; Oude Elberink, J N G; Gawlik, R; Jakob, T; Kosnik, M; Lange, J; Mingomataj, E; Mitsias, D I; Mosbech, H; Ollert, M; Pfaar, O; Pitsios, C; Pravettoni, V; Roberts, G; Ruëff, F; Sin, B A; Asaria, M; Netuveli, G; Sheikh, A

2017-03-01

The European Academy of Allergy and Clinical Immunology (EAACI) is in the process of developing the EAACI Guidelines on Allergen Immunotherapy (AIT) for the management of insect venom allergy. To inform this process, we sought to assess the effectiveness, cost-effectiveness and safety of AIT in the management of insect venom allergy. We undertook a systematic review, which involved searching 15 international biomedical databases for published and unpublished evidence. Studies were independently screened and critically appraised using established instruments. Data were descriptively summarized and, where possible, meta-analysed. Our searches identified a total of 16 950 potentially eligible studies; of which, 17 satisfied our inclusion criteria. The available evidence was limited both in volume and in quality, but suggested that venom immunotherapy (VIT) could substantially reduce the risk of subsequent severe systemic sting reactions (OR = 0.08, 95% CI 0.03-0.26); meta-analysis showed that it also improved disease-specific quality of life (risk difference = 1.41, 95% CI 1.04-1.79). Adverse effects were experienced in both the build-up and maintenance phases, but most were mild with no fatalities being reported. The very limited evidence found on modelling cost-effectiveness suggested that VIT was likely to be cost-effective in those at high risk of repeated systemic sting reactions and/or impaired quality of life. The limited available evidence suggested that VIT is effective in reducing severe subsequent systemic sting reactions and in improving disease-specific quality of life. VIT proved to be safe and no fatalities were recorded in the studies included in this review. The cost-effectiveness of VIT needs to be established. © 2016 The Authors. Allergy Published by John Wiley & Sons Ltd.

Vitamin E levels in preeclampsia placenta tissue and its correlation with oxidative stress injury and apoptosis

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Jun Li

2017-04-01

Full Text Available Objective: To study the vitamin E levels in preeclampsia placenta tissue and its correlation with oxidative stress injury and apoptosis. Methods: A total of 60 pregnant women with preeclampsia who received treatment and gave birth in our hospital between July 2012 and January 2016 were collected and divided into mild preeclampsia group (n=41 and severe preeclampsia group (n=19 according to the disease severity; 38 normal pregnant women who received pregnancy test and gave birth in our hospital during the same period were selected as healthy control group. The placental tissue samples of three groups of research subjects were retained, high performance liquid chromatograph-mass spectrometry was used to detect VitE levels in tissue grinding fluid, automatic biochemical analyzer was used to detect the levels of oxidative stress injury indexes, and fluorescence quantitative PCR method was used to detect the mRNA expression of apoptosis molecules. Results: VitE, SOD and CAT levels in grinding fluid of severe preeclampsia group were lower than those of mild preeclampsia group and healthy control group while ROS and AOPP levels were higher than those of mild preeclampsia group and healthy control group; Fas, caspase and Apaf-1 mRNA expression were higher than those of mild preeclampsia group and healthy control group while anti-apoptotic molecules Bcl-2, Bcl-xl, Mcl-2 and p57kip2 mRNA expression were lower than those of mild preeclampsia group and healthy control group. Spearman correlation analysis showed that VitE level in the preeclampsia placenta tissue was directly correlated with oxidative stress injury and cell apoptosis. Conclusion: VitE deficiency is the direct factor that results in oxidative stress and cell apoptosis in patients with preeclampsia, and the VitE supplementation in time is expected to become the auxiliary treatment means for patients with preeclampsia.

The association between the Index of Nutritional Quality (INQ) and breast cancer and the evaluation of nutrient intake of breast cancer patients: A case-control study.

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Vahid, Farhad; Hatami, Mahshid; Sadeghi, Mahya; Ameri, Fatemeh; Faghfoori, Zeinab; Davoodi, Sayed Hossein

2018-01-01

Breast cancer (BrCa) is the most frequently diagnosed cancer among females and second cancer after lung cancer in many societies. In Iran, the risk for BrCa is 1 in 35 and each year, 8000 new patients have been diagnosed with BrCa. Studies have shown that dietary components are implicated in the etiology of BrCa. The Index of Nutritional Quality (INQ) is a method of quantitative and qualitative analysis of single foods, meals, and diets. The aim of this study was to determine the usefulness of INQs in predicting BrCa risk. Our case-control study was conducted from March 2015 to February 2016. The study included 145 cases and 148 controls who attended the Shahid Beheshti University of Medical Sciences Cancer Research Center. INQ scores were computed based on dietary intake using a validated 168-item food frequency questionnaire. Logistic regression models were used to estimate multivariable odds ratios adjusted body mass index, education, employment, marital status, menarche age, childbirth number, smoking, menopause status, and physical activity. Vitamins A, C, B 1 , B 2 , and B 12 and selenium INQs as a continuous variable in relation to risk for BrCa showed a significant association after multivariate adjustment (odds ratio [OR] vitA , 0.41 [0.27-0.64]; OR vitC , 0.30 [0.20-0.47]; OR vitB1 , 0.08 [0.04-0.17]; OR vitB2 , 0.19 [0.11-0.34]; OR vitB12 , 0.44 [0.31-0.61]; and OR selenium , 0.42 [0.26-0.67]). Women who consumed a healthier diet including vitamin A, β-carotene, vitamin C, and folate and low-fat milk were at decreased risk for developing BrCa compared with those whose diet included more high fat and lamb meat. Copyright © 2017 Elsevier Inc. All rights reserved.

Effects of chromium and chromium + vitamin C combination on metabolic, oxidative, and fear responses of broilers transported under summer conditions

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Perai, A. H.; Kermanshahi, H.; Moghaddam, H. Nassiri; Zarban, A.

2015-04-01

A total of 240 female broilers (42 days old) were randomly assigned to four groups with six replicates and fed either a basal diet (two control groups) or a basal diet supplemented with either 1,200 μg Cr+3 from chromium (Cr) methionine/kg (Cr group) or 1,200 μg Cr+3 from Cr methionine plus 800 mg vitamin C (Vit C)/kg of diet (Cr + Vit C group). After 7 days on the dietary treatment, all groups except one of the controls were transported for 3 h under the summer conditions. Performance parameters were not influenced by dietary treatments. The plasma concentrations of insulin, triiodothyronine, triglyceride, and the ratio of triiodothyronine/thyroxin were decreased and the ratio of glucose/insulin was increased due to transport process. Road transportation also increased the plasma concentrations of protein, cholesterol, aspartate aminotransferase, and creatine kinase and decreased the concentration of low-density lipoprotein cholesterol in the Cr + Vit C group. The pretransport concentrations of insulin and triiodothyronine were highest in the Cr + Vit C group. The concentration of phosphorous was lower in the Cr group than that in the other groups after transport. No significant effects of dietary treatments were observed on the other biochemical parameters. Transport increased malondialdehyde concentration in the control group and did not change plasma total antioxidant capacity and erythrocyte glutathione peroxidase activity. Either in combination or alone, Cr increased plasma total antioxidant capacity (before transport P ≤ 0.05, after transport P = 0.07) but did not affect the concentration of malondialdehyde and activity of glutathione peroxidase. The duration of tonic immobility (TI) was similar between nontransported control chicks and transported chicks without any supplements. Pretreatment with Cr + Vit C significantly reduced the duration of TI.

Sulphated galactopyran derived from Gracilaria opuntia, a marine macroalgae restores the antioxidant metabolic enzymes during STZ induced diabetic rats

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Lavanya Rayapu

2017-02-01

Full Text Available Objective: To screen the effect of sulphated galactopyran fraction isolated from Gracilaria opuntia (G. opuntia (FM4 in streptozotocin (STZ induced diabetic rats. Methods: In vitro antioxidant assays of FM4 were estimated by DPPH, ABTS, hydroxyl free radical and Nitric oxide free radical activities. FM4 was purified and characterized by 1H-NMR spectra and FTIR as sulphated galactopyran. Diabetes was induced intraperitonially by single dose of STZ (55 mg/kg body weight. FM4 was administrated orally (80, 100, 125 mg/kg BW to diabetic rats for 60 days. The enzymatic and non-enzymatic antioxidants such as superoxide dismutase (SOD, glutathione peroxidase (GPx, catalase (CAT, glutathione-S-transferase (GST, lipid peroxidase (LPx, glutathione reduced (GSH, vitamin-C (VIT-C and vitamin-E (VIT-E levels were estimated. Glibenclamide was used as standard drug. Results: Our results demonstrated that the aqueous extract of G. opuntia possess free radical scavenging activity. During FM4 fraction treatment (100 mg/kg BW, the SOD, GPx, CAT, GST, GSH, VIT-C and VIT-E levels were significantly (P < 0.05 increased, and the LPx levels were decreased in different organs such as liver, kidney, brain and pancreas of diabetic rats. Conclusions: The sulphated galactopyran fraction of the marine macroalgae (G. opuntia possesses the antioxidant activity which might help in the prevention of oxidative damage that occurs during diabetes.

Assessing Timing and Causes of Neonatal Lamb Losses in a Bighorn Sheep ( Ovis canadensis canadensis ) Herd via Use of Vaginal Implant Transmitters.

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Grigg, Jamin L; Wolfe, Lisa L; Fox, Karen A; Killion, Halcyon J; Jennings-Gaines, Jessica; Miller, Michael W; P Dreher, Brian

2017-07-01

We evaluated the use of vaginal implant transmitters (VITs) as a means of detecting, capturing, and radio collaring Rocky Mountain bighorn sheep ( Ovis canadensis canadensis) lambs to estimate survival and to facilitate carcass recovery to assess causes of mortality. We focused on one of several bighorn herds in Colorado, US, suffering from depressed recruitment that was not preceded by a classic all-age die-off. We captured, radio-collared, diagnosed pregnancy by ultrasound examination, and inserted VITs into 15 pregnant ewes from a herd residing near Granite, Colorado. We were subsequently able to collar a lamb from each of 13 VITs, and two additional lambs opportunistically from ewes without transmitters. As lambs died, we recovered and submitted carcasses for necropsy and laboratory assessment. All lambs captured and one additional lamb (carcass found opportunistically) were dead by about 130 d of age: 11 died of apparent pneumonia (all within 8-10 wk of age), one died from trauma after being kicked or trampled, one was killed by a mountain lion ( Puma concolor ), and three died of starvation likely caused by abandonment after capture. Pneumonic lambs had involvement of Mycoplasma ovipneumoniae and leukotoxigenic Bibersteinia trehalosi . The use of VITs and lamb collars enabled us to efficiently identify pneumonia as the predominant cause of depressed lamb recruitment in this herd; however, we urge care in neonatal lamb handling to minimize abandonment.

No prognostic value added by vitamin D pathway SNPs to current prognostic system for melanoma survival.

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Li Luo

Full Text Available The prognostic improvement attributed to genetic markers over current prognostic system has not been well studied for melanoma. The goal of this study is to evaluate the added prognostic value of Vitamin D Pathway (VitD SNPs to currently known clinical and demographic factors such as age, sex, Breslow thickness, mitosis and ulceration (CDF. We utilized two large independent well-characterized melanoma studies: the Genes, Environment, and Melanoma (GEM and MD Anderson studies, and performed variable selection of VitD pathway SNPs and CDF using Random Survival Forest (RSF method in addition to Cox proportional hazards models. The Harrell's C-index was used to compare the performance of model predictability. The population-based GEM study enrolled 3,578 incident cases of cutaneous melanoma (CM, and the hospital-based MD Anderson study consisted of 1,804 CM patients. Including both VitD SNPs and CDF yielded C-index of 0.85, which provided slight but not significant improvement by CDF alone (C-index = 0.83 in the GEM study. Similar results were observed in the independent MD Anderson study (C-index = 0.84 and 0.83, respectively. The Cox model identified no significant associations after adjusting for multiplicity. Our results do not support clinically significant prognostic improvements attributable to VitD pathway SNPs over current prognostic system for melanoma survival.

Nutritional rickets: vitamin D, calcium, and the genetic make-up.

Science.gov (United States)

El Kholy, Mohamed; Elsedfy, Heba; Fernández-Cancio, Monica; Hamza, Rasha Tarif; Amr, Nermine Hussein; Ahmed, Alaa Youssef; Toaima, Nadin Nabil; Audí, Laura

2017-02-01

The prevalence of vitamin D (vitD) deficiency presenting as rickets is increasing worldwide. Insufficient sun exposure, vitD administration, and/or calcium intake are the main causes. However, vitD system-related genes may also have a role. Prospective study: 109 rachitic children completed a 6-mo study period or until rachitic manifestations disappeared. Thirty children were selected as controls. Clinical and biochemical data were evaluated at baseline in patients and controls and biochemistry re-evaluated at radiological healing. Therapy was stratified in three different protocols. Fifty-four single-nucleotide polymorphisms (SNPs) of five vitD system genes (VDR, CP2R1, CYP27B1, CYP24A1, and GC) were genotyped and their association with clinical and biochemcial data was analyzed. Therapy response was similar in terms of radiological healing although it was not so in terms of biochemical normalization. Only VDR gene (promoter, start-codon, and intronic genotypes) was rickets-associated in terms of serum 25-OH-D, calcium, radiological severity and time needed to heal. Eight patients with sufficient calcium intake and 25-OH-D levels carried a VDR genotype lacking minor allele homozygous genotypes at SNPs spread along the gene. Although patients presented epidemiologic factors strongly contributing to rickets, genetic modulation affecting predisposition, severity, and clinical course is exerted, at least in part, by VDR gene polymorphic variation.

Antioxidant effect of vitamin E and 5-aminosalicylic acid on acrylamide induced kidney injury in rats

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Rajeh, Nisreen A.; Al-Dhaheri, Najlaa M.

2017-01-01

Objectives: To explore renal toxicity caused by sub-acute exposure of acrylamide and to study the protective effect of 5-Aminosalicylic acid (5-ASA) and Vitamin E (vit-E)on Acrylamide (ACR) induced renal toxicity. Methods: This study was conducted at King Fahad Medical Research Centre, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia, between August and November 2015. A total of 49 adult Wistar rats (250 ± 20g) aged 60 days were kept in a controlled environment and used in the present study. The rats were divided into 7 groups (control, ACR alone, ACR+5-ASA, ACR+vit-E, ACR+ASA+vit-E, vit-E alone, and ASA alone). After 5 days of ACR oral gavage treatment, the rats were observed for 24 hours then killed. Histopathology for the kidney and lactate dehydrogenase assay were carried out. Results: Acrylamide produced significant pathological changes in the kidney with acute tubular necrosis in the distal tubules that could be reversed by concomitant injection of rat with 5-ASA. Together with vitamin E, 5-ASA, showed maximum renal protection. No statistically significant difference was observed in either body weights or lactate dehydrogenase activity of ACR treated rats. Conclusion: Acrylamide exposure leads to adverse clinical pathologies of renal tubules, which were reversed by a concomitant treatment with 5-ASA and vitamin-E PMID:28133684

Vitamina C restaura pressão arterial e a resposta vasodilatadora no antebraço em crianças obesas Vitamina C restaura presión arterial y respuesta vasodilatadora en el antebrazo en niños obesos Vitamin C restores blood pressure and vasodilator response during mental stress in obese children

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Pricilla Regina Oliveira Fernandes Fernandes

2011-06-01

Full Text Available FUNDAMENTO: A resposta vasodilatadora periférica tem um papel importante na fisiopatologia da obesidade e das doenças cardíacas. OBJETIVO: Avaliar o efeito crônico da suplementação de vitamina C (VitC sobre a pressão arterial e na resposta vasodilatadora ao estresse mental. MÉTODOS: Neste estudo prospectivo, randomizado e duplo cego foram avaliadas crianças obesas, de ambos os gêneros e com idade entre 8 a 12 anos divididas em 2 grupos: 1 grupo de crianças suplementadas com 500 mg de vitamina C (n = 11 e, 2 substância placebo (n = 10 durante 45 dias. Oito crianças eutróficas, pareadas por idade também foram arroladas no estudo. Foi avaliada a pressão arterial média (PAM, frequência cardíaca (ECG e fluxo sanguíneo no antebraço pela plestimografia de oclusão venosa. A condutância vascular no antebraço (CVA foi obtida através da relação entre o fluxo sanguíneo no antebraço e a PAM (X100. RESULTADOS: Antes da intervenção, as crianças obesas apresentaram PAM maior e CVA menor quando comparadas às crianças eutróficas. Pós-intervenção, o Grupo VitC apresentou redução da PAM no repouso (81 ± 2 vs 75 ± 1 mmHg, p = 0,01, enquanto no Grupo Placebo não houve alteração da PAM (p = 0,58. Adicionalmente, VitC promoveu um aumento da CVA no repouso (3,40 ± 0,5 vs 5,09 ± 0,6 un, p = 0,04 e durante o estresse mental (3,92 ± 0,5 vs 6,68 ± 0,9 un, p = 0,03. Além disso, pós suplementação com VitC, os níveis da CVA foram estatisticamente semelhantes aos das crianças eutróficas no repouso (5,09 ± 0,6 vs 5,82 ± 0,4 un, p > 0,05 e durante o estresse mental (6,68 ± 0,9 vs 7,35 ± 0,5 un, p > 0,05. CONCLUSÃO: Suplementação com VitC reduziu a pressão arterial e restabeleceu a resposta vasodilatadora periférica em crianças obesas.FUNDAMENTO: La respuesta vasodilatadora periférica tiene un papel importante en la fisiopatología de la obesidad y de las enfermedades cardíacas. OBJETIVO: Evaluar el efecto cr

Effect of dietary vitamin E and selenium supplementation on semen quality in Cairn Terriers with normospermia

DEFF Research Database (Denmark)

Kirchhoff, K. T.; Failing, K.; Goericke-Pesch, S.

2017-01-01

Among others, selenium (Se) and vitamin E (VitE) have been provided to dogs to improve semen quality. However, scientific evidence documenting an effect in dogs is lacking. The aim of this study was to investigate the effect of supplementation of these antioxidants on various ejaculate parameters......). Although some effects could be identified for selected parameters, we failed to identify a clear trend about how a 3 months VitE and/or Se supplementation affects semen parameters in normospermic Cairn Terriers....

Vitamin D2 Supplementation Amplifies Eccentric Exercise-Induced Muscle Damage in NASCAR Pit Crew Athletes

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Nieman, David C.; Gillitt, Nicholas D.; Shanely, R. Andrew; Dew, Dustin; Meaney, Mary Pat; Luo, Beibei

2013-01-01

This study determined if 6-weeks vitamin D2 supplementation (vitD2, 3800 IU/day) had an influence on muscle function, eccentric exercise-induced muscle damage (EIMD), and delayed onset of muscle soreness (DOMS) in National Association for Stock Car Auto Racing (NASCAR) NASCAR pit crew athletes. Subjects were randomized to vitD2 (n = 13) and placebo (n = 15), and ingested supplements (double-blind) for six weeks. Blood samples were collected and muscle function tests conducted pre- and post-study (leg-back and hand grip dynamometer strength tests, body weight bench press to exhaustion, vertical jump, 30-s Wingate test). Post-study, subjects engaged in 90 min eccentric-based exercise, with blood samples and DOMS ratings obtained immediately after and 1- and 2-days post-exercise. Six weeks vitD2 increased serum 25(OH)D2 456% and decreased 25(OH)D3 21% versus placebo (p creatine phosphokinase 24 h post-exercise, 169%, 32%, p athletes following eccentric exercise. PMID:24362707

Relationship between fresh-packaged spinach leaves exposed to continuous light or dark and bioactive contents: effects of cultivar, leaf size, and storage duration.

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Lester, Gene E; Makus, Donald J; Hodges, D Mark

2010-03-10

Current retail marketing conditions allow produce to receive artificial light 24 h per day during its displayed shelf life. Essential human-health vitamins [ascorbic acid (vit C), folate (vit B(9)), phylloquinone (vit K(1)), alpha-tocopherol (vit E), and the carotenoids lutein, violaxanthin, zeaxanthin, and beta-carotene (provit A)] also are essential for photosynthesis and are biosynthesized in plants by light conditions even under chilling temperatures. Spinach leaves, notably abundant in the aforementioned human-health compounds, were harvested from flat-leaf 'Lazio' and crinkle-leafed 'Samish' cultivars at peak whole-plant maturity as baby (top- and midcanopy) and larger (lower-canopy) leaves. Leaves were placed as a single layer in commercial, clear-polymer retail boxes and stored at 4 degrees C for up to 9 days under continuous light (26.9 micromol.m(2 ).s) or dark. Top-canopy, baby-leaf spinach generally had higher concentrations of all bioactive compounds, on a dry weight basis, with the exception of carotenoids, than bottom-canopy leaves. All leaves stored under continuous light generally had higher levels of all bioactive compounds, except beta-carotene and violaxanthin, and were more prone to wilting, especially the flat-leafed cultivar. All leaves stored under continuous darkness had declining or unchanged levels of the aforementioned bioactive compounds. Findings from this study revealed that spinach leaves exposed to simulated retail continuous light at 4 degrees C, in clear plastic containers, were overall more nutritionally dense (enriched) than leaves exposed to continuous darkness.

Vitamin E protects against the mitochondrial damage caused by cyclosporin A in LLC-PK1 cells

International Nuclear Information System (INIS)

Arriba, G. de; Perez de Hornedo, J.; Ramirez Rubio, S.; Calvino Fernandez, M.; Benito Martinez, S.; Maiques Camarero, M.; Parra Cid, T.

2009-01-01

Cyclosporin A (CsA) has nephrotoxic effects known to involve reactive oxygen species (ROS), since antioxidants prevent the kidney damage induced by this drug. Given that mitochondria are among the main sources of intracellular ROS, the aims of our study were to examine the mitochondrial effects of CsA in the porcine renal endothelial cell line LLC-PK1 and the influence of the antioxidant Vitamin E (Vit E). Following the treatment of LLC-PK1 cells with CsA, we assessed the mitochondrial synthesis of superoxide anion, permeability transition pore opening, mitochondrial membrane potential, cardiolipin peroxidation, cytochrome c release and cellular apoptosis, using flow cytometry and confocal microscopy procedures. Similar experiments were done after Vit E preincubation of cells. CsA treatment increased superoxide anion in a dose-dependent way. CsA opened the permeability transition pores, caused Bax migration to mitochondria, and decreased mitochondrial membrane potential and cardiolipin content. Also CsA released cytochrome c into cytosol and provoked cellular apoptosis. Vit E pretreatment inhibited the effects that CsA induced on mitochondrial structure and function in LLC-PK1 cells and avoided apoptosis. CsA modifies mitochondrial LLC-PK1 cell physiology with loss of negative electrochemical gradient across the inner mitochondrial membrane and increased lipid peroxidation. These features are related to apoptosis and can explain the cellular damage that CsA induces. As Vit E inhibited these effects, our results suggest that they were mediated by an increase in ROS production by mitochondria.

Participação dos entes federados no financiamento da saúde bucal de atenção básica: estudo no município da Vitória de Santo Antão, Pernambuco

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José Eudes de Lorena Sobrinho

2013-12-01

Full Text Available O objetivo deste estudo foi mensurar o percentual de participação dos entes federados (União, Estado e Município no financiamento das ações e serviços de saúde bucal na atenção básica no município da Vitória de Santo Antão, Pernambuco, no ano de 2010 a partir da apuração dos custos. Utilizou-se a metodologia do custeio por absorção, tendo sido os custos agrupados em blocos: recursos humanos, recursos materiais e pensos com finalidade odontológica, custos prediais e manutenção de equipamentos. Foram consultados os repasses federais e estaduais através dos sistemas de informação de acesso público (fundo nacional de saúde e fundo estadual de saúde para se estimar a participação dos governos no custeio das ações e serviços de saúde bucal na atenção básica. De um total de R$ 1.340.307,80 (um milhão, trezentos e quarenta mil, trezentos e sete reais e oitenta centavos referentes ao custo total, 37,5% foram relativos a recursos financeiros federais e 62,5% aos municipais, não sendo observada a participação estadual. Constata-se que o município continua sendo o principal provedor do custeio da saúde bucal na atenção básica, direcionando a necessidade de uma articulação interfederativa mais efetiva que assegure o financiamento tripartite.

The effects of fish oil capsules and vitamin B1 tablets on duration and severity of dysmenorrhea in students of high school in Urmia-Iran.

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Hosseinlou, A; Alinejad, V; Alinejad, M; Aghakhani, N

2014-09-18

The purpose of this study is the comparison of the effect of vitamin B1 and fish oil with together on severity and duration of dysmenorrhea, and if it is effective, we can administrative both of them with less complication to compare with other chemical drugs which has many disadvantages. High school of Urmia city, between March 2008 and June 2008. This study has a double-blind clinical trial design.240 high school female students with dysmenorrhea by a randomized Method were followed up in a double-blind, randomized, placebo-controlled study by dividing into four groups with 60 members. The daily supplement was vit B1 (100 mg/day and fish oil pearl 500 mg/day), taken as a single dose starting at the beginning of the menstrual cycle and continued for 2 consecutive months. Intensity of pain in three experimental groups (Vit B1, fish oil and both of them) had significant difference comparing placebo group and intensity of pain had reduced. (p<0.001), (p=0.018), (p<0.001) VS in placebo group (p=0.79). Duration of pain had significantly reduced in all three experimental groups compared with placebo group. (p=0.004), (p=0.008), (p<0.001) VS in placebo group (p=0.32). In all of drugs, results (mean) was better at the end of the second month than the first month. Fish oil and vit B1 effects on treatment of primary dysmenorrhea were similar, but vit B1 has less complications and it was more acceptable. We mixed them and compared its results with vit B1, fish oil and placebo separately. Pain duration is the least in Vitamin B1 tablets compare with the others, but its duration was minimum in complex of Vitamin B1 tablets and fish oil capsules. Due to good effects of vitamin B1 and fish oil on symptoms of dysmenorrhea, using of them is suggested.

A 'difficult' insect allergy patient: reliable history of a sting, but all testing negative.

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Tracy, James M; Olsen, Jonathan A; Carlson, John

2015-08-01

Few conditions are as treatable as allergy to stinging insects, with venom immunotherapy (VIT) providing up to 98% protection to subsequent stings. The challenge with VIT is not in the treatment, but in the diagnosis. To offer VIT, one must determine a history of a systemic reaction to a stinging insect in conjunction with the presence venom-specific IgE. Current diagnostic methods, although sensitive and specific, are imperfect, and some newer testing options are not widely available. A conundrum occasionally faced is the patient with a reliable and compelling history of a systemic allergic reaction yet negative venom-specific testing. This diagnostic dilemma presents an opportunity to consider possible causes for this diagnostic challenge. Our evolving understanding of the role of occult mast cell disease may begin to help us understand this situation and develop appropriate management strategies. Venom-specific skin testing has long been the cornerstone of the evaluation of venom sensitivity and is often combined with in-vitro assays to add clarity, but even these occasionally may fall short. Exploring novel venom diagnostic testing methods may help to fill in some of the diagnostic gaps. Do currently available venom vaccines contain all the key venom species? Are there enough differences between insect species that we may simply be missing the relevant allergens? What is the significance of the antigenicity of carbohydrate moieties in venoms? What is the role of recombinant venom extracts? VIT is the definitive treatment for insect allergic individuals. To utilize VIT, identification of the relevant Hymenoptera is necessary. Unfortunately, this cannot always be accomplished. This deficiency can have several causes: a potential comorbid condition such as occult mast cell disease, limitations of currently available diagnostic resources, or testing vaccines with an insufficient coverage of relevant venom allergens. Exploring these potential causes may help to

Degree of conversion and microhardness of TPO-containing resin-based composites cured by polywave and monowave LED units.

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Santini, Ario; Miletic, Vesna; Swift, Michael D; Bradley, Mark

2012-07-01

To determine the degree of conversion (DC) and Knoop microhardness (KHN) of resin-based composites (RBCs) containing trimethylbenzoyl-diphenylphosphine oxide (TPO) cured by polywave or monowave LED light-curing units (LCUs). Three groups (each n = 5) of Tetric EvoCeram (Ivoclar Vivadent), Vit-l-escence (Ultradent) and Herculite XRV Ultra (Kerr) were prepared in Teflon moulds (5mm in diameter and 2mm thick) and cured with polywave Bluephase(®) G2 (Ivoclar Vivadent), polywave Valo (Ultradent) or monowave Bluephase(®) (Ivoclar Vivadent; control) resulting in 9 groups. DC and KHN were determined using micro-Raman spectroscopy and Knoop microhardness, respectively. High-performance liquid chromatography and nuclear magnetic resonance spectroscopy were used to confirm the presence or absence of TPO in the three uncured materials. Data were statistically analysed using two-way and one-way ANOVA and DC and KHN were correlated using Pearson's correlation (α = 0.05). TPO was confirmed in Tetric EvoCeram and Vit-l-escence but not in Herculite XRV Ultra. All three LCUs produced comparable KHN for Tetric EvoCeram and Herculite XRV Ultra (p > 0.05). Both polywave LCUs resulted in significantly higher KHN for Vit-l-escence and higher DC in Tetric EvoCeram and Vit-l-escence than the monowave Bluephase(®) (p Conversely, Bluephase(®) showed higher DC than the two polywave LCUs in Herculite XRV Ultra (p conversion and KHN in the two TPO-containing RBCs, but not in Herculite XRV Ultra. DC and KHN were linearly correlated in all three RBCs. Vit-l-escence showed the highest DC and KHN of the three materials tested. The use of polywave LEDs significantly improves both the DC and KHN of materials which contain TPO. This should be taken into account when curing bleached shades of RBCs even if the manufacturers do not indicate the presence of TPO in their materials. Copyright © 2012 Elsevier Ltd. All rights reserved.

Effect of dietary supplementation with Rhizopus oryzae or Chrysonilia crassa on growth performance, blood profile, intestinal microbial population, and carcass traits in broilers exposed to heat stress

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S. Sugiharto

2017-09-01

Full Text Available Dietary supplementation of additives has recently been part of strategies to deal with the detrimental effects of heat stress (HS on the performance and carcass traits in broiler chicks. This study aimed to investigate the effect of dietary supplementation with the fungi Rhizopus oryzae or Chrysonilia crassa on growth, blood profile, intestinal microbial population and carcass traits in broiler chicks subjected to HS. R. oryzae and C. crassa are filamentous fungi isolated from the ileum of indigenous Indonesian chickens which exhibited probiotic and antioxidant properties. Two hundred and forty 21-day-old male broiler chicks were randomly allotted into six groups, including birds reared under normal temperature (28 ± 2 °C (CONT, birds reared under HS conditions (35 ± 2 °C (HS-CONT, birds reared under HS and provided with commercial anti-stress formula (HS-VIT, birds reared under HS and provided with R. oryzae (HS-RO, birds reared under HS and provided with C. crassa (HS-CC and birds reared under HS and provided with rice bran (HS-RB. Body weight gain was highest (P < 0. 01 and lowest (P < 0. 01 in CONT and HS-CONT birds, respectively. The heart was heavier (P < 0. 05 in CONT than in HS-CONT and HS-VIT birds. CONT birds had heavier duodenum (P < 0. 05 and jejunum (P < 0. 01 than other birds. Eosinophils was higher (P < 0. 05 in HS-CC than in other birds. Low-density lipoprotein (LDL was higher (P < 0. 05 in HS-CONT than in CONT, HS-VIT and HS-CC birds. Total triglyceride was highest (P < 0. 05 and lowest (P < 0. 05 in HS-RB and HS-RO birds, respectively. Alanine aminotransferase (ALT was higher (P < 0. 05 in HS-CONT than in other HS birds. Total protein was lowest and highest (P < 0. 05 in CONT and HS-CONT birds, respectively. Albumin was higher (P < 0. 05 in HS-CONT and HS-VIT than in HS-RO birds. Globulin was lower (P < 0. 05 in CONT than in HS

The angular distributions of ultraviolet spectral irradiance at different solar elevation angles under clear sky conditions

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Liu, Yan; Hu, LiWen; Wang, Fang; Gao, YanYan; Zheng, Yang; Wang, Yu; Liu, Yang

2016-01-01

To investigate the angular distributions of UVA, UVB, and effective UV for erythema and vitamin D (vitD) synthesis, the UV spectral irradiances were measured at ten inclined angles (from 0° to 90°) and seven azimuths (from 0° to 180°) at solar elevation angle (SEA) that ranged from 18.8° to 80° in Shanghai (31.22° N, 121.55° E) under clear sky and the albedo of ground was 0.1. The results demonstrated that in the mean azimuths and with the back to the sun, the UVA, UVB, and erythemally and vitD-weighted irradiances increased with the inclined angles and an increase in SEA. When facing toward the sun at 0°-60° inclined angles, the UVA first increased and then decreased with an increase in SEA; at other inclined angles, the UVA increased with SEA. At 0°-40° inclined angles, the UVB and erythemally and vitD-weighted irradiances first increased and then decreased with an increase in SEA, and their maximums were achieved at SEA 68.7°; at other inclined angles, the above three irradiances increased with an increase in SEA. The maximum UVA, UVB, and erythemally and vitD-weighted irradiances were achieved at an 80° inclined angle at SEA 80° (the highest in our measurements); the cumulative exposure of the half day achieved the maximum at a 60° inclined angle, but not on the horizontal. This study provides support for the assessment of human skin sun exposure.

Effect of metformin combined with clomiphene on insulin resistance, oxidative stress response and T cell immune response in patients with PCOS

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Xiao-Lian Zhang

2017-08-01

Full Text Available Objective: To study the effect of metformin combined with clomiphene on insulin resistance, oxidative stress response and T cell immune response in patients with polycystic ovarian syndrome (PCOS. Methods: A total of 94 patients who were diagnosed with PCOS in Jingzhou Second People’s Hospital between September 2014 and October 2016 were selected and randomly divided into the combined group who received the metformin combined with clomiphene therapy and the control group who received clomiphene therapy. The insulin resistance, oxidative stress response and T cell immune response were evaluated before treatment and 3 menstrual cycles after treatment. Results: 3 menstrual cycles after treatment, HOMA-IR level, serum F-Ins, F-CP, TOS, MDA, AOPP and IL-17 contents as well as peripheral blood RORγt mRNA expression of combined group were significantly lower than those before treatment while HOMA-β level, serum TAS, SOD, GSH-Px, VitC, VitE, IL-10 and TGF-β1 contents as well as peripheral blood Foxp3 mRNA expression were significantly higher than those before treatment; HOMA-IR and HOMA-β levels, serum F-Ins, F-CP, TOS, MDA, AOPP, IL-17, TAS, SOD, GSH-Px, VitC, VitE, IL-10 and TGF-β1 contents as well as peripheral blood Foxp3 and RORγt mRNA expression of control group were not different from those before treatment. Conclusion: Metformin combined with clomiphene can significantly improve the insulin resistance, oxidative stress response and T cell immune response in patients with PCOS.

The place of Ruscus extract, hesperidin methyl chalcone, and vitamin C in the management of chronic venous disease.

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Jawien, Arkadiusz; Bouskela, Eliete; Allaert, François A; Nicolaïdes, Andrew N

2017-02-01

Despite continuous improvement in our knowledge and management of chronic venous disease (CVD), certain areas, such as the role of muscarinic receptors in the pathology and treatment of CVD, remain unexplored. The symposium "The place of Ruscus extract, hesperidin methyl chalcone, and vitamin C in the management of CVD", held at the Annual Meeting of the European Venous Forum on 7-9 July 2016 in London, presented an update on the pathophysiology of CVD and highlighted how the combination of Ruscus extract, hesperidin methyl chalcone, and vitamin C (Ruscus/HMC/VitC; Cyclo 3® Fort), may counteract the deleterious processes underlying CVD. The data presented during this symposium are reported here. The pathophysiology of CVD is driven by a complex process involving numerous factors, with the two key players being venous hypertension and the inflammatory response. The cascade of reactions induced by disturbed venous flow, inflammation, and tissue alterations results in the early appearance of symptoms and progressive development of clinical signs of disease. Previous studies have shown that Ruscus extract acts at three levels: on the veins, capillaries and lymphatics, and has anti-inflammatory properties. A series of recent experiments has shed new light on the mechanism of action of the combination of Ruscus/HMC/VitC. The efficacy of Ruscus/HMC/VitC in CVD is supported by clinical studies, while two meta-analyses have confirmed a significant decrease of several symptoms and ankle circumference in response to treatment with this agent, leading to the conclusion that Ruscus/HMC/VitC deserves a Grade A rating.

Natural history of Hymenoptera venom allergy in children not treated with immunotherapy.

Science.gov (United States)

Lange, Joanna; Cichocka-Jarosz, Ewa; Marczak, Honorata; Krauze, Agnieszka; Tarczoń, Izabela; Świebocka, Ewa; Lis, Grzegorz; Brzyski, Piotr; Nowak-Węgrzyn, Anna

2016-03-01

Differences in treatment approach still exist for children after systemic sting reactions. In addition, there are still some doubts about when systemic reactors should be treated with venom immunotherapy (VIT). To determine the rate of sting recurrence and natural history of Hymenoptera venom allergy (HVA) in children not treated with VIT. A total of 219 children diagnosed as having HVA who were not treated with VIT were identified in 3 pediatric allergology centers. Survey by telephone or mail with the use of a standardized questionnaire was conducted. The number of field re-stings, subsequent symptoms, and provided treatment were analyzed. A total of 130 of the 219 patients responded to the survey, for a response rate of 59.4%. During the median follow-up period of 72 months (interquartile range, 52-85 months), 44 children (77% boys) were stung 62 times. Normal reactions were most common, occurring in 27 patients (62%). Severe systemic reactions (SSRs) occurred in 8 (18%) of those who were re-stung. The subsequent reaction was significantly milder (P insect (P insect (P = .03). In children with SSRs, median time from diagnosis to re-sting was 2 times longer than that in those with large local reactions and normal reactions (P = .007). Most children with HVA not treated with VIT reported milder reactions after a re-sting. Probability of SSR to re-sting increases along with the severity of initial reaction. Copyright © 2016 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

Impact of Western and Mediterranean Diets and Vitamin D on Muscle Fibers of Sedentary Rats

Science.gov (United States)

Purrello, Francesco

2018-01-01

Background: The metabolic syndrome is associated with sarcopenia. Decreased serum levels of Vitamin D (VitD) and insulin-like growth factor (IGF)-1 and their mutual relationship were also reported. We aimed to evaluate whether different dietary profiles, containing or not VitD, may exert different effects on muscle molecular morphology. Methods: Twenty-eight male rats were fed for 10 weeks in order to detect early defects induced by different dietary regimens: regular diet (R); regular diet with vitamin D supplementation (R-DS) and regular diet with vitamin D restriction (R-DR); high-fat butter-based diets (HFB-DS and HFB-DR) with 41% energy from fat; high-fat extra-virgin olive oil-based diets (HFEVO-DS and HFEVO-DR) with 41% energy from fat. IL-1β, insulin-like growth factor (IGF)1, Dickkopf-1 (DKK-1), and VitD-receptor (VDR) expressions were evaluated by immunohistochemistry. Muscle fiber perimeter was measured by histology and morphometric analysis. Results: The muscle fibers of the HEVO-DS rats were hypertrophic, comparable to those of the R-DS rats. An inverse correlation existed between the dietary fat content and the perimeter of the muscle fibers (p < 0.01). In the HFB-DR rats, the muscle fibers appeared hypotrophic with an increase of IL-1β and a dramatic decrease of IGF-1 expression. Conclusions: High-fat western diet could impair muscle metabolism and lay the ground for subsequent muscle damage. VitD associated with a Mediterranean diet showed trophic action on the muscle fibers. PMID:29462978

In vitro testing to diagnose venom allergy and monitor immunotherapy: a placebo-controlled, crossover trial.

Science.gov (United States)

Brown, S G A; Haas, M A; Black, J A; Parameswaran, A; Woods, G M; Heddle, R J

2004-05-01

In people with a history of sting allergy, only prior reaction severity and older age are known to predict subsequent reaction risk. Furthermore, no diagnostic test other than a deliberate sting challenge has been found to identify people in whom venom immunotherapy (VIT) has been unsuccessful. We aimed to assess the utility of a number of in vitro tests to diagnose venom allergy and to monitor immunotherapy. During a double-blind randomized placebo-controlled crossover trial of Myrmecia pilosula ant VIT the following venom-specific tests were performed at enrolment, and at completion of treatment prior to a diagnostic sting challenge; leucocyte stimulation index (SI), IL-4 production, IgE RAST, histamine release test (HRT), leukotriene release test (LRT) and basophil activation test (BAT). Intradermal venom skin testing (VST) was also performed at trial entry. Only VST and HRT identified those at risk of sting anaphylaxis in the placebo group. Although IgE RAST, leucocyte SI and IL-4 production, LRT and BAT all correlated well with intradermal VSTs, they did not predict sting challenge outcome. After successful VIT, venom-induced leucocyte IL-4 production tended to fall, whereas IgE RAST increased and a natural decline in HRT reactivity was reversed. A confounding seasonal affect on laboratory results was suspected. The HRT warrants further assessment for diagnosis of venom allergy. Uninformative performance of the commercially available LRT and BAT tests may be due to pre-incubation with IL-3. None of the tests evaluated appear to be reliable markers of successful VIT.

Reversible reprotoxic effects of manganese through DAF-16 transcription factor activation and vitellogenin downregulation in Caenorhabditis elegans.

Science.gov (United States)

Gubert, Priscila; Puntel, Bruna; Lehmen, Tassia; Bornhorst, Julia; Avila, Daiana S; Aschner, Michael; Soares, Felix A A

2016-04-15

Vitellogenesis is the yolk production process which provides the essential nutrients for the developing embryos. Yolk is a lipoprotein particle that presents lipids and lipid-binding proteins, referred to as vitellogenins (VIT). The Caenorhabditis elegans nematode has six genes encoding VIT lipoproteins. Several pathways are known to regulate vitellogenesis, including the DAF-16 transcription factor. Some reports have shown that heavy metals, such as manganese (Mn), impair brood size in C. elegans; however the mechanisms associated with this effect have yet to be identified. Our aim was to evaluate Mn's effects on C. elegans reproduction and better understand the pathways related to these effects. Young adult larval stage worms were treated for 4h with Mn in 85mM NaCl and Escherichia coli OP50 medium. Mn reduced egg-production and egg-laying during the first 24h after the treatment, although the total number of progenies were indistinguishable from the control group levels. This delay may have occurred due to DAF-16 activation, which was noted only after the treatment and was not apparent 24h later. Moreover, the expression, protein levels and green fluorescent protein (GFP) fluorescence associated with VIT were decreased soon after Mn treatment and recovered after 24h. Combined, these data suggest that the delay in egg-production is likely regulated by DAF-16 and followed by the inhibition of VIT transport activity. Further studies are needed to clarify the mechanisms associated with Mn-induced DAF-16 activation. Copyright © 2016 Elsevier Inc. All rights reserved.

Mechanism for microstructural evolution induced by high temperature deformation in Zr-based bulk metallic glasses

International Nuclear Information System (INIS)

Cheng, Sirui; Wang, Chunju; Ma, Mingzhen; Shan, Debin; Guo, Bin

2016-01-01

In the Zr_4_1_._2Ti_1_3_._8Cu_1_2_._5Ni_1_0Be_2_2_._5 (Vit1) alloy undergoing high temperature deformation, its thermal properties and microstructure are quite different from those in the annealing alloy. In order to research the coupled effect of temperature and plastic strain on microstructural evolution of Zr-based amorphous, uniaxial compression test of Vit1 alloy with good amorphous nature has been performed, and then the structural state and thermal properties of Vit1 alloy after thermal deformation and isothermal annealing in the supercooled liquid region were investigated. It is revealed that the deformed specimens possess higher characteristic temperature and lower enthalpy change of microstructural relaxation. In addition, the smaller inter-atomic distance and higher order degree of atomic arrangement can be observed in those deformed Vit1 alloy. That can be deduced that thermal deformation is in favor of the microstructural evolution from a metastable amorphous state to stable crystallization state, because plastic strain promotes the annihilation of free volume and provide excess driving force of atomic diffusion. However, upon increasing the ambient temperature, the influence of plastic deformation on microstructure gradually decreased owing to the decreasing proportion of the plastic deformation-induced annihilation of free volume during the whole thermal deformation process. - Highlights: • The deformed specimens possess closer microstructure and higher characteristic temperatures. • The order degree of microstructures in deformed specimens is higher than that in annealed specimens. • Thermal deformation accelerates the microstructural evolution of Zr-based BMGs. • The influence of thermal deformation on microstructure decreases with the temperature increasing.

Dietary sources and sociodemographic and lifestyle factors affecting vitamin D and calcium intakes in European adolescents: the Healthy Lifestyle in Europe by Nutrition in Adolescence (HELENA) Study.

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Julián, Cristina; Mouratidou, Theodora; Vicente-Rodriguez, Germán; Gracia-Marco, Luis; Valtueña, Jara; González-Gross, Marcela; Ferrari, Marika; Gottrand, Frederic; Manios, Yannis; de la O, Alejandro; Widhalm, Kurt; Molnár, Dénes; Kafatos, Antonios; Sjöström, Michael; Kersting, Mathilde; Gunter, Marc J; De Henauw, Stefaan; Moreno, Luis A; Huybrechts, Inge

2017-06-01

To investigate dietary sources of Ca and vitamin D (VitD) intakes, and the associated sociodemographic and lifestyle factors, among European adolescents. Linear regression mixed models were used to examine sex-specific associations of Ca and VitD intakes with parental education, family affluence (FAS), physical activity and television (TV) watching while controlling for age, Tanner stage, energy intake and diet quality. The Healthy Lifestyle in Europe by Nutrition in Adolescence (HELENA)Cross-Sectional Study. Adolescents aged 12·5-17·5 years (n 1804). Milk and cheese were the main sources of Ca (23 and 19 % contribution to overall Ca intake, respectively). Fish products were the main VitD source (30 % contribution to overall VitD intake). Ca intake was positively associated with maternal education (β=56·41; 95 % CI 1·98, 110·82) and negatively associated with TV viewing in boys (β=-0·43; 95 % CI -0·79, -0·07); however, the significance of these associations disappeared when adjusting for diet quality. In girls, Ca intake was positively associated with mother's (β=73·08; 95 % CI 34·41, 111·74) and father's education (β=43·29; 95 % CI 5·44, 81·14) and FAS (β=37·45; 95 % CI 2·25, 72·65). This association between Ca intake and mother's education remained significant after further adjustment for diet quality (β=41·66; 95 % CI 0·94, 82·38). Girls with high-educated mothers had higher Ca intake. Low-educated families with poor diet quality may be targeted when strategizing health promotion programmes to enhance dietary Ca.

Mechanism for microstructural evolution induced by high temperature deformation in Zr-based bulk metallic glasses

Energy Technology Data Exchange (ETDEWEB)

Cheng, Sirui [School of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001 (China); Wang, Chunju [Key Laboratory of Micro-Systems and Micro-Structures Manufacturing, Ministry of Education, Harbin Institute of Technology, Harbin 150080 (China); School of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001 (China); Ma, Mingzhen [State Key Laboratory of Metastable Materials Science and Technology, Yanshan University, Qinhuangdao 066004 (China); Shan, Debin, E-mail: shandebin@hit.edu.cn [State Key Laboratory of Advanced Welding and Joining, Harbin Institute of Technology, Harbin 150001 (China); Key Laboratory of Micro-Systems and Micro-Structures Manufacturing, Ministry of Education, Harbin Institute of Technology, Harbin 150080 (China); School of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001 (China); Guo, Bin [School of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001 (China)

2016-08-15

In the Zr{sub 41.2}Ti{sub 13.8}Cu{sub 12.5}Ni{sub 10}Be{sub 22.5} (Vit1) alloy undergoing high temperature deformation, its thermal properties and microstructure are quite different from those in the annealing alloy. In order to research the coupled effect of temperature and plastic strain on microstructural evolution of Zr-based amorphous, uniaxial compression test of Vit1 alloy with good amorphous nature has been performed, and then the structural state and thermal properties of Vit1 alloy after thermal deformation and isothermal annealing in the supercooled liquid region were investigated. It is revealed that the deformed specimens possess higher characteristic temperature and lower enthalpy change of microstructural relaxation. In addition, the smaller inter-atomic distance and higher order degree of atomic arrangement can be observed in those deformed Vit1 alloy. That can be deduced that thermal deformation is in favor of the microstructural evolution from a metastable amorphous state to stable crystallization state, because plastic strain promotes the annihilation of free volume and provide excess driving force of atomic diffusion. However, upon increasing the ambient temperature, the influence of plastic deformation on microstructure gradually decreased owing to the decreasing proportion of the plastic deformation-induced annihilation of free volume during the whole thermal deformation process. - Highlights: • The deformed specimens possess closer microstructure and higher characteristic temperatures. • The order degree of microstructures in deformed specimens is higher than that in annealed specimens. • Thermal deformation accelerates the microstructural evolution of Zr-based BMGs. • The influence of thermal deformation on microstructure decreases with the temperature increasing.

Effect of VitaminA on Development of Ductus Arteriosus in Fatal Rabbit%维生素A对胎兔动脉导管发育的影响

Institute of Scientific and Technical Information of China (English)

李涛; 王宏伟; 潘军; 邹典定; 肖玉; 徐少勇

2006-01-01

目的研究维生素A(VitA)对胎兔动脉导管发育的影响,为防治早产儿动脉导管未闭(PDA)寻找新的途径.方法孕兔8只随机分为4组,VitA 5000 IU/d,受孕d25服用1次(A组),孕d25、d26连续服用2次(B组),孕2周后隔天服用(C组)和对照组服用生理盐水(D组).取胎兔动脉导管固定,切片、HE染色,观察导管内膜增殖情况,测定导管内钙浓度.结果A、B、C组导管内膜增殖程度较D组明显增加,A、B、C组导管内钙浓度明显增加.结论VitA能增加导管内钙浓度,促进胎兔动脉导管发育.

Effects of turmeric (Curcuma longa) and vitamin E on histopathological lesions induced in bursa of Fabricius of broiler chicks by salinomycin.

Science.gov (United States)

Sayrafi, Reza; Mirzakhani, Navideh; Mobaseri, Reza

2017-01-01

The aim of this study was to evaluate the protective effects of the turmeric in comparison to vitamin E on bursal damages induced by salinomycin in broiler chickens. In this study, forty one day-old broiler chicks were randomly divided into four treatment groups: 1- basal diet as control, 2- basal diet plus salinomycin, 3- basal diet plus salinomycin (SLM) and vitamin E (Vit. E) and 4- basal diet plus salinomycin and turmeric powder. The chicks were treated for two weeks. At the end of the experiment, the bursal tissues were removed and fixed in 10% formalin solution. Tissue sections were stained with hematoxylin and eosin stain for histopathological studies. Light microscopic observations showed that, SLM diminished cortex thickness of bursal tissue, enhanced its medulla zone and caused severe lymphocytic necrosis. In addition, SLM led to fibrosis of interstitium along with sever edema of medulla zone in the bursal tissue of the chicken. Administration of Vit. E and TP significantly inhibited the SLM-induced derangements and comparing the Vit. E and TP showed no significant differences. The results of this study indicated that the turmeric may protect bursa of Fabricius against toxicity induced by salinomycin in chicks.

Bioavailability of Some Micro nutrient in Male Liver Albino Rat Exposed to one of Organo chlorine Compounds

International Nuclear Information System (INIS)

Sharada, H.M.; El-Nabarawy, F.E.; Abdalla, M.S.; Ayad, S.K.Y.; Mohamed, R.Y.

2011-01-01

Trichloroethylene (TCE) is widely used in our daily life as a degreasing metal agent, pesticides, dry cleaning, typewriter, dis infectious and cleaning agents. The continuous exposure to it can induce liver injury with damage in hepatocytes that manifested by significant alterations in liver biochemical markers, immunological responses, histopathological pictures and DNA fragmentation. The present study aimed to evaluate the potential ability of daily supplementation with some micro nutrients as vit. C and/or Zn in protection of hepatocytes beside ameliorative effect of the damage events that induced in hepatocytes in response to TCE exposure. The results of the current study showed significant improvement in liver biochemical parameters with balance in immunological disturbance beside reduction in alterations of histopathological pictures and DNA fragmentation towards the normal side as a consequence of daily supplementation with vit. C and/or Zn which was obvious when both micro nutrients taken together. The current data could concluded that daily supplementation with both vit. C and Zn together may protect and alleviate the destructive events induced in hepatocytes in response of continuous exposure to TCE particularly when individuals dealing with this toxic material in their daily life.

Tissue distribution and functional analysis of vitellogenin-6 of Toxocara canis.

Science.gov (United States)

Zhu, Hong-Hong; Ma, Guang-Xu; Luo, Yong-Fang; Luo, Yong-Li; Yin, Sha-Sha; Xiong, Yi; Zhou, Rong-Qiong

2017-06-01

Toxocara canis is an common intestinal nematode of canids and the principal causative agent of human toxocariasis. Vitellogenin (Vg), a source of amino acids and lipids in the eggs, are considered to play an important role in embryo development of a wide range of organisms. In the present study, the transcriptional levels of Tc-vit-6 gene in male and female adult T. canis were determined by quantitative real-time PCR, which indicated high transcription of Tc-vit-6 in the intestine, reproductive tract and body wall of male and female adult T. canis. The fragment of Tc-vit-6 encoding a vWD domain, was cloned and expressed to produce a rabbit anti-TcvWD polyclonal antibody. Tissue distribution of TcVg6 was detected by immunohistochemical assays, which showed predominant distribution of TcVg6 in the tissues of intestine, as well as reproductive tract (including some of the germ cells) and musculature of male and female adult worms. Collectively, these results indicated multiple biological roles of TcVg6 apart from that in the reproduction of T. canis. Copyright © 2017 Elsevier Inc. All rights reserved.

N-terminal aliphatic residues dictate the structure, stability, assembly, and small molecule binding of the coiled-coil region of cartilage oligomeric matrix protein.

Science.gov (United States)

Gunasekar, Susheel K; Asnani, Mukta; Limbad, Chandani; Haghpanah, Jennifer S; Hom, Wendy; Barra, Hanna; Nanda, Soumya; Lu, Min; Montclare, Jin Kim

2009-09-15

The coiled-coil domain of cartilage oligomeric matrix protein (COMPcc) assembles into a homopentamer that naturally recognizes the small molecule 1,25-dihydroxyvitamin D(3) (vit D). To identify the residues critical for the structure, stability, oligomerization, and binding to vit D as well as two other small molecules, all-trans-retinol (ATR) and curcumin (CCM), here we perform an alanine scanning mutagenesis study. Ten residues lining the hydrophobic pocket of COMPcc were mutated into alanine; of the mutated residues, the N-terminal aliphatic residues L37, L44, V47, and L51 are responsible for maintaining the structure and function. Furthermore, two polar residues, T40 and Q54, within the N-terminal region when converted into alanine improve the alpha-helical structure, stability, and self-assembly behavior. Helical stability, oligomerization, and binding appear to be linked in a manner in which mutations that abolish helical structure and assembly bind poorly to vit D, ATR, and CCM. These results provide not only insight into COMPcc and its functional role but also useful guidelines for the design of stable, pentameric coiled-coils capable of selectively storing and delivering various small molecules.

Importance of basophil activation testing in insect venom allergy

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Kosnik Mitja

2009-12-01

Full Text Available Abstract Background Venom immunotherapy (VIT is the only effective treatment for prevention of serious allergic reactions to bee and wasp stings in sensitized individuals. However, there are still many questions and controversies regarding immunotherapy, like selection of the appropriate allergen, safety and long term efficacy. Methods Literature review was performed to address the role of basophil activation test (BAT in diagnosis of venom allergy. Results In patients with positive skin tests or specific IgE to both honeybee and wasp venom, IgE inhibition test can identify sensitizing allergen only in around 15% and basophil activation test increases the identification rate to around one third of double positive patients. BAT is also diagnostic in majority of patients with systemic reactions after insect stings and no detectable IgE. High basophil sensitivity to allergen is associated with a risk of side effects during VIT. Persistence of high basophil sensitivity also predicts a treatment failure of VIT. Conclusion BAT is a useful tool for better selection of allergen for immunotherapy, for identification of patients prone to side effects and patients who might be treatment failures. However, long term studies are needed to evaluate the accuracy of the test.

Study on changes of serum ferritin (SF), folic acid (FA) and vitamin B12 levels after transfusion of preserved red cells (PRC) in patients with iron deficiency anemia (IDA)

International Nuclear Information System (INIS)

Liu Hongsu; Li Xinhua; Li Keqin

2008-01-01

Objective: To explore the changes of serum SF, FA and VitB 12 levels after transfusion of preserved red cells in patients with IDA. Methods: Serum SF, FA and VitB 12 levels (with RIA) were detected both before and after transfusion of preserved red cells in 32 patients with IDA as well as in 35 controls. Results: Before transfusion of preserved red cells, the serum SF levels were significantly lower than those in controls (P 12 levels were significantly higher (P 12 were not much different from those in the controls. Conclusion: Transfusion of preserved red cells proves to be very useful. (authors)

Mechanical properties of Zr41.2Ti13.8Ni10Cu12.5Be22.5 bulk metallic glass with different geometric confinements

Science.gov (United States)

Zhang, Changqin; Zhang, Haifeng; Sun, Qilei; Liu, Kegao

2018-03-01

Zr41.2Ti13.8Ni10Cu12.5Be22.5 (Vit 1) bulk metallic glass with Cu sleeves at different positions was prepared by the Cu mold casting method, and the effects of different geometric confinements offered by Cu sleeves on the mechanical properties of Vit 1 were investigated. It was found that the mechanical properties were prominently influenced by different geometric confinements and the plasticity could be modified by optimizing the positions of Cu sleeves. The results revealed that shear band initiation and propagation could be efficiently intervened by changing the radial boundary restraints, which led to quite different mechanical behaviors.

Studies of the polynuclear complexes of labile ligands of vitamin B1 and Zn(II), Cd(II) and Hg(II) with Fe(III)

International Nuclear Information System (INIS)

Ojo, J.O.

2003-01-01

The ligands (complex salts) of vitamin B/sub 1/ (H Vit.) and the chlorides of Zn, Cd and Hg with the general formula, [H Vit]/sup +2/ [MCl/sub 4/]/sup -2/ were prepared and their interactions with iron (III) investigated. It was found that the complex salts of Zn and Cd produced the dinuclear complexes and that of mercury produced a complex without the thiamine moiety. The possible reason for the absence of a Hg complex similar to those of Zn and Cd may be that large size of mercury ion. The complexes were characterized by elementary analyses, infrared and visible spectra, magnetic moment and conductivity measurements.(author)

Studies on the efficacy of vitamin c and saccharomyces cerevisiae in providing partial protection against ochratoxicosis in broiler chickens

International Nuclear Information System (INIS)

Hatab, M.H.M.

2011-01-01

This study was carried out at the experimental animal house and laboratory belonging to poultry physiology unit, Biological Application Department, Atomic Energy Authority, Egypt from November to December(2008). The aim of this work was to investigate the effects of supplementing broiler diets with active dried yeast; Saccharomyces cerevisiae (Sc), vitamin C (Vit.C) or their combination to alleviate the deleterious effect of ochratoxin A (OA) on productive performance, weights, functional and histological profile of internal organ and physiological and immunological parameters. A total number of 368 sexed male Ross broiler chicks were used in this study. The chicks were randomly distributed according to diet supplementation into eight groups (46 chicks each) from 1 day old to 5 weeks of age. The results obtained showed that presence OA at 200 ppb in broiler diets caused significant decrease in body weight, feed consumption and worse feed conversion ratio, hemoglobin, packed cell volume of blood, red and white blood cell counts, blood proteins, thyroid hormones, immune response and weights of thymus and bursa and alteration in histological picture of kidney, liver and bursa. At the same time, a significant increase in relative weights of kidney, liver, gizzard, proventriculus, AST, ALT enzymes, uric acid, creatinine in addition to high mortality percentage. Addition Sc either alone or in combination with vit.C to OA- fed groups alleviated the toxic effects of OA on productive performance, immunological, hematological, biochemical and histological parameters. On the other hand, the addition of vit.C to OA- contaminated broiler diet did not prevent the negative effects of OA observed in chicks, except for some measurements as body weight, mortality percentage and blood proteins, although less extent was shown compared to the Sc or Sc + vit.C supplementation.

Características físicas e sensoriais de clones de batata-doce Physical and sensorial characteristics of sweetpotato clones

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Adriana Dias Cardoso

2007-12-01

Full Text Available Com o objetivo de avaliar propriedades físicas e sensoriais de clones de batata-doce em Vitória da Conquista - BA foi realizado este experimento, composto por 16 clones oriundos de Janaúba- G, Viçosa - MG, Bom Jardim de Minas - MG, Gurupi - TO, Santo Antônio da Platina - PR, Holambra II - SP, Vitória da Conquista - BA e Condeúba - BA. Utilizou-se o delineamento em blocos casualizados, com 16 tratamentos e 3 repetições. Avaliaram-se as características sensoriais: aparência, umidade, doçura, coloração da polpa, dificuldade de deglutição das raízes tuberosas e as características físicas: tempo de cozimento e peso específico. Os dados foram submetidos à análise de variância e teste de Scott-Knott a 5% de probabilidade, entretanto, as características sensoriais foram obtidas apenas em valores de porcentagem. O clone 25 apresentou as melhores características sensoriais e o clone 7 apresentou melhor tempo de cozimento.The aim of this experiment was to evaluate the physical and sensorial characteristics of sweetpotato clones in Vitória da Conquista, Bahia State, Brazil. Sixteen clones were analyzed, originating from Janaúba, MG, Viçosa, MG; Bom Jardim de Minas, MG; Gurupi, TO; Santo Antônio da Platina, PR; Holambra II, SP; Vitória da Conquista, BA; and Condeúba, BA. One utilized randomized blocks with 16 treatments and three repetitions. The following characteristics were analyzed: aspect, humidity, sweetness, color, deglutition difficulty, cooking and specific gravity of the storage roots. The data were submitted to variance analysis using a ScottKnott test with 5% probability. Clone 25 presented the best sensorial characteristics, and clone 7 presented the best cooking time.

The effect of metformin treatment on the serum levels of homocysteine, folic acid, and vitamin B12 in patients with polycystic ovary syndrome

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Sedigheh Esmaeilzadeh

2017-01-01

Full Text Available Background and Objective: Hyperhomocysteinemia is a well-known risk factor for cardiovascular disease. Although metformin therapy can increase homocysteine (Hcy levels, it frequently is used as an oral medicine in women with polycystic ovary syndrome (PCOS, who might be at risk of catching diabetes mellitus. The aim of this study was to investigate the effect of metformin on the levels of serum Hcy, vitamin B12 (vit B12, and folic acid in patients with PCOS. Materials and Methods: An interventional study was designed with 18 patients with PCOS at the Fatemehzahra infertility Hospital in Babol, Iran. Metformin treatment (500 mg twice daily was initiated in all patients for a period of consecutive 6 months. The levels of serum Hcy, vit B12, and folic acid were measured in the participants before and after metformin treatment. Results: The mean vit B12 level showed a significant decrease in patients after 6 months of metformin treatment (P = 0.002. However, there was no significant difference in serum folic acid levels. The mean Hcy levels increased after treatment, but this difference not was statistically significant. When patients were stratified into four subgroups by their insulin sensitivity and body mass index (BMI, relatively similar results were obtained in the subgroups, except that Hcy levels in the overweight/obesity group (BMI > 25 kg/m2 after treatment showed a significant increase (P = 0.01. Conclusion: These findings indicate that metformin increases the serum Hcy concentration in patients with PCOS especially in the women with BMI > 25 kg/m2. The possible mechanism for this effect would be the obvious reduction in the levels of vit B12.

Allergen immunotherapy: clinical and practical education of Italian trainees in allergy and clinical immunology schools.

Science.gov (United States)

Ridolo, E; Incorvaia, C; Senna, G E; Montagni, M; Olivieri, E; Canonica, G W

2013-10-01

We performed a survey, based on a questionnaire including 20 items, submitted anonymously to Italian trainees in Allergology and Clinical Immunology, in order to obtain information about their specific allergen immunotherapy (AIT) practices. The questionnaire was sent to 40 trainees, who had attended the last two years of the training course. Thirty-four subjects (mean age: 27 years, 65% females) adequately completed the survey. The answers to the questionnaire showed that only 60% of the training programs included lectures on AIT. Among the trainees using AIT, only 40% declared being able to prescribe it independently, while 60% were guided by a tutor. Of the trainees who were able to prescribe AIT autonomously, 60% were familiar with both routes of administration, i.e. subcutaneous (SCIT) and sublingual immunotherapy (SLIT), while 25% of these used only SLIT. In 80% of the training institutions involved, the trainees could attend a dedicated AIT outpatient ward for SCIT administration; only 40% administered AIT personally, and in half of these cases, they were guided by a tutor. Only 70% of trainees had experience in the follow-up of patients still under treatment and of patients who had completed treatment. Analysis of the answers obtained for questions on venom immunotherapy (VIT) showed that, in 90% of cases, the trainees attended a dedicated outpatients ward where VIT is administered, but with a role limited to observation/cooperation. Only 30% were involved in the follow-up of patients who were under treatment or who had completed VIT. Only 20% of the trainees felt confident enough about VIT to prescribe this treatment independently, 80% knew there were several administration protocols, and the majority prescribed products from three different manufacturers. These findings suggest that there is significant room for improving the instructions provided regarding allergology and clinical immunology to trainees in Italy with respect to AIT.

IgE-Api m 4 Is Useful for Identifying a Particular Phenotype of Bee Venom Allergy.

Science.gov (United States)

Ruiz, B; Serrano, P; Moreno, C

Different clinical behaviors have been identified in patients allergic to bee venom. Compound-resolved diagnosis could be an appropriate tool for investigating these differences. The aims of this study were to analyze whether specific IgE to Api m 4 (sIgE-Api m 4) can identify a particular kind of bee venom allergy and to describe response to bee venom immunotherapy (bVIT). Prospective study of 31 patients allergic to bee venom who were assigned to phenotype group A (sIgE-Api m 4 Api m 4 ≥0.98 kU/L), treated with purified aqueous (PA) extract. Sex, age, cardiovascular risk, severity of preceding sting reaction, exposure to beekeeping, and immunological data (intradermal test, sIgE/sIgG4-Apis-nApi m 1, and sIgE-rApi m 2-Api m 4 were analyzed. Systemic reactions (SRs) during bVIT build-up were analyzed. Immunological and sting challenge outcomes were evaluated in each group after 1 and 2 years of bVIT. Phenotype B patients had more severe reactions (P=.049) and higher skin sensitivity (P=.011), baseline sIgE-Apis (P=.0004), sIgE-nApi m 1 (P=.0004), and sIgG4-Apis (P=.027) than phenotype A patients. Furthermore, 41% of patients in group B experienced SRs during the build-up phase with NA; the sting challenge success rate in this group was 82%. There were no significant reductions in serial intradermal test results, but an intense reduction in sIgE-nApi m 1 (P=.013) and sIgE-Api m 4 (P=.004) was observed after the first year of bVIT. Use of IgE-Api m 4 as the only discrimination criterion demonstrated differences in bee venom allergy. Further investigation with larger populations is necessary.

Antioxidant-mediated up-regulation of OGG1 via NRF2 induction is associated with inhibition of oxidative DNA damage in estrogen-induced breast cancer

International Nuclear Information System (INIS)

Singh, Bhupendra; Chatterjee, Anwesha; Ronghe, Amruta M; Bhat, Nimee K; Bhat, Hari K

2013-01-01

Estrogen metabolism-mediated oxidative stress is suggested to play an important role in estrogen-induced breast carcinogenesis. We have earlier demonstrated that antioxidants, vitamin C (Vit C) and butylated hydroxyanisole (BHA) inhibit 17β-estradiol (E2)-mediated oxidative stress and oxidative DNA damage, and breast carcinogenesis in female August Copenhagen Irish (ACI) rats. The objective of the present study was to characterize the mechanism by which above antioxidants prevent DNA damage during breast carcinogenesis. Female ACI rats were treated with E2; Vit C; Vit C + E2; BHA; and BHA + E2 for up to 240 days. mRNA and protein levels of a DNA repair enzyme 8-Oxoguanine DNA glycosylase (OGG1) and a transcription factor NRF2 were quantified in the mammary and mammary tumor tissues of rats after treatment with E2 and compared with that of rats treated with antioxidants either alone or in combination with E2. The expression of OGG1 was suppressed in mammary tissues and in mammary tumors of rats treated with E2. Expression of NRF2 was also significantly suppressed in E2-treated mammary tissues and in mammary tumors. Vitamin C or BHA treatment prevented E2-mediated decrease in OGG1 and NRF2 levels in the mammary tissues. Chromatin immunoprecipitation analysis confirmed that antioxidant-mediated induction of OGG1 was through increased direct binding of NRF2 to the promoter region of OGG1. Studies using silencer RNA confirmed the role of OGG1 in inhibition of oxidative DNA damage. Our studies suggest that antioxidants Vit C and BHA provide protection against oxidative DNA damage and E2-induced mammary carcinogenesis, at least in part, through NRF2-mediated induction of OGG1

The Usefullness of Green Coconut Water Supplementation in Overcoming The Toxic Effect of Mercury Chloride in The Blood and Blood Profile (Study in Sprague Dewly Rats

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Abdulrzag Ehmeeda

2017-07-01

Full Text Available When people are exposed to mercury chloride, it can produce a variety of health effects in the blood and liver. Coconut water contains Zn, Fe, Vit. C, Vit B11, Vit. B6­, and Se to reduce mercury chloride level in the blood and improve blood profile. Aim of this study was to analysis the effect of green coconut water supplementation in overcoming the toxic effect of Hg chloride in the blood of Sprague dawley rats exposed to Hg chloride. Samples were randomly about 36 animals rats exposed to HgCl2 through forced feeding by 20 mg/kgBW sondage perday for 14 days, which divided into control group, and intervention groups were given fresh green coconut water in each by 6, 8, and 10 mL/kgBW for intervention 7 and 17 days. The result of this study showed that there is a significant effect and the decrease in mercury levels in the blood. There is no significant affect on the hemoglobin level, hematocrit level and platelet count with the treatment of green coconut water in the rats with exposure Hg. New findings/significance of contribution were the effectiveness of green coconut water to reduce Hg level in blood and improve blood profile.

[Role of vitamin D in female reproduction].

Science.gov (United States)

DE Leo, Vincenzo; Cappelli, Valentina; Morgante, Giuseppe; DI Sabatino, Alessandra

2017-11-10

La vit. D, o calcitriolo, è stata identificata inizialmente nei primi anni '20 ed ha un ruolo chiave nella regolazione del metabolismo osseo, nell'omeostasi del calcio e del fosforo e nel promuovere la mineralizzazione ossea. Le azioni della vit. D sono mediante tramite un recettore nucleare (VDR), che è espresso in numerosi organi come l'intestino, l'osso e il rene, ma anche nelle gonadi, nel seno, nel pancreas, nel sistema cardiovascolare, nel cervello (microglia) e nelle cellule del sistema immunitario. In tutti questi tessuti bersaglio non classici la 1,25(OH)2D sembra avere un ruolo nel ridurre il rischio di alcune malattie croniche (incluso il cancro, le infezioni, le patologie autoimmuni e cardiache) attraverso la modulazione della crescita cellulare, un'azione neuromuscolare, immunitaria e antiinfiammatoria. Il VDR agisce soprattutto attraverso la regolazione dell'espressione di quei geni i cui promotori contengono specifiche sequenza DNA note come vitamin D response elements (VDRE). La vit. D ha un ruolo chiave sia nella fisiologia della riproduzione che nello sviluppo di varie patologie ginecologiche come la PCOS, l'endometriosi, la fibromatosi e anche di tipo ostetrico (pre-eclampsia, diabete gestazionale) e negli ultimi tempi sta assumendo un ruolo importante nelle tecniche di fecondazione in vitro.

Vitamin E supplementation of undernourished ewes pre- and post-lambing reduces weight loss of ewes and increases weight of lambs.

Science.gov (United States)

Rosales Nieto, César Augusto; Meza-Herrera, César Alberto; Moron Cedillo, Felipe de Jesús; Flores Najera, Manuel de Jesús; Gámez Vázquez, Hector Guillermo; Ventura Pérez, Felipe de Jesús; Liu, Shimin

2016-03-01

The aim of this study was to test if vitamin E supplementation during late gestation and early lactation would affect the weight of ewes under nutritional restriction and the performance of their lambs. Mature Rambouillet ewes (n = 37) were fed a diet that supplied 70 % of the energy and 80 % of recommended protein requirements and randomly assigned to either vitamin E (vit E, n = 20, 4 IU of α-tocopherol kg(-1) of live weight) or control (n = 17, without vitamin E supplementation). During the experimental period, the mean weight of ewes decreased from 74.6 ± 2.4 to 58.1 ± 2.2 kg. Weight loss of ewes was slightly less for the vit E than the control (-65 vs -124 g day(-1), SEM = 46; P = 0.07). Lambs born from vit E-supplemented ewes were heavier than lambs from the control and grew significantly faster (239 vs 195 g day(-1), SEM = 29.3, P ewes are not met, supplementation of vitamin E during late gestation and early lactation might be an effective strategy to minimise ewe weight loss as well as to increase lamb growth.

Effects of Combined Vigorous Interval Training Program and Diet on Body Composition, Physical Fitness, and Physical Self-Perceptions Among Obese Adolescent Boys and Girls.

Science.gov (United States)

Rey, Olivier; Vallier, Jean-Marc; Nicol, Caroline; Mercier, Charles-Symphorien; Maïano, Christophe

2017-02-01

This study examined the effects of a five-week intervention combining vigorous interval training (VIT) with diet among twenty-four obese adolescents. Fourteen girls and ten boys (aged 14-15) schooled in a pediatric rehabilitation center participated. The VIT intensity was targeted and remained above 80% of maximal heart rate (HR) and over six kilocalories per minute. Pre- and postintervention measures were body composition (BMI, weight, body fat percentage), physical self-perceptions (PSP), physical fitness (6-min walking distance and work) and its associated physiological responses (HR peak and blood lactate concentration). A series of two-way analyses of variance or covariance controlling for weight loss were used to examine the changes. Significant improvements were found in body composition, physical fitness and PSP (endurance, activity level, sport competence, global physical self-concept and appearance). In addition, boys presented higher levels of perceived strength and global physical self-concept than girls. Finally, there was a significant increase in perceived endurance, sport competence, and global physical self-concept in girls only. This five-week VIT program combined with diet represents an effective means for improving body composition, physical fitness, and PSP in obese adolescents, the effects on PSP being larger among girls.

Nevenbestanddelen in margarine en halvarine

NARCIS (Netherlands)

Essers, M.L.; Muuse, B.G.

1981-01-01

In het margarinebesluit staan de toevoegingsmiddelen vermeld die toegestaan zijn in margarine en halvarine. Voor de conserveermiddelen, kleurstoffen en retinol (vit. A) is een bepalingsvoorschrift opgenomen in de warenwet.

Produtos de acerola: estudo da estabilidade de vitamina C West indian cherry products: study of vitamin C stability

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Fábio Yamashita

2003-04-01

Full Text Available A acerola é um fruto altamente perecível e que contém altos teores de vitamina C, sendo este o seu principal atrativo em termos nutricionais. A atual legislação brasileira prevê uma variação de, no máximo, 20% do teor dos nutrientes especificados no rótulo. Devido a essa exigência seria importante que os fabricantes considerassem tanto o teor inicial de vitamina C quanto a perda ao longo da armazenagem dos produtos de acerola. Neste trabalho, foi feito o acompanhamento da estabilidade da vitamina C em polpa pasteurizada e acerola in natura congeladas, ambas armazenadas a -12ºC e -18ºC, e em suco de acerola pasteurizado engarrafado, mantido a temperatura ambiente, ao longo de 4 meses de armazenagem. As polpas congeladas não apresentaram degradação significativa durante este período, já as in natura apresentaram cinética de degradação de 1ª ordem e o suco de ordem zero. Após 4 meses de armazenagem as acerolas armazenadas a -12ºC e -18ºC apresentaram teores de 869±12 e 1.223±148 mg vit.C/100g, representando uma perda de 43% e 19%, respectivamente, em relação ao teor inicial. Polpas a -12ºC e -18ºC apresentaram teores de 1.314±6 e 1.322±2 mg vit.C/100g, respectivamente, representando uma perda de, aproximadamente, 3% e o suco apresentou uma perda de 32%, correspondendo a um teor final de 673±17mg vit.C/100g.West indian cherry is a very perishable fruit and its vitamin C content is its principal nutritional appeal. Brazilian legislation admits a 20% variation in the nutrient content from the one specified on the label. Due to this requirement the initial vitamin C content and losses during storage should be known in products. Vitamin C stability in pasteurized pulp and west indian cherry in natura, both frozen and stored at -12ºC e -18ºC and of the pasteurized juice stored at room temperature, were determined during four months. Frozen pulps had no significant vitamin C degradation during this period and fruits in

A mixed Ni(II) ionic complex containing V-shaped water trimer ...

Indian Academy of Sciences (India)

aDepartment of Chemical Engineering, Sunchon National University, 315 Maegok Dong, ... bMaterials Chemistry Division, School of Advanced Sciences, VIT University, Vellore 632014, ..... Metal Carboxylates, Ph.D. Thesis, Indian Institute.

[Effects of flunarizine and vitamin C on hemodynamics in rat heart subjected to ischemia-reperfusion].

Science.gov (United States)

Xian, Y; Lan, T; Wang, Y

1998-09-01

Langendorff perfusion isolated rat heart was subjected to total global ischemia (coronary flow rate is equal to zero) for 10 minutes and reperfusion for 15 minutes. The heart rate (HR), left ventricular developed pressure (LVDP), coronary flow rate (CFR), electrocardiogram (ECG) and the effects of calcium antagonist-flunarizine (FNZ) and/or oxygen free radical scavenger--vitamine C on the above parameters were observed. The results showed that FNZ dilated coronary vessel (P Vitamine C did not affect HR, LVP and CFR. The recovery of the product of HR and LVDP-Rate Pressure Product (RPP) in the FNZ + Vit. C group, Vit. C group and FNZ group was significantly higher than that in the control group (P C may improve the recovery of heart function after reperfusion.

Eleições 2004: uma análise do desempenho partidário no Brasil

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Dejalma Cremonese

2005-01-01

Full Text Available O artigo extrai dos resultados das eleições municipais 2004 alguns elementos para análise. Dentre eles, a clara visualização de que o PT e o PSDB saem fortalecidos e o PMDB e PFL vêem declinar sua participação política no cenário nacional. Apesar dos resultados finais apontarem para a vitória do PT em nível nacional (valor quantitativo, o partido foi derrotado na sua principal “vitrine” administrativa, Porto Alegre. Já a vitória do PSDB foi significativa no quesito “qualidade” (ganho ideológico, principalmente no valor simbólico de ter conquistado a maior capital do país, São Paulo.

ACTION OF VITAMIN E ON EXPERIMENTAL SEVERE ACUTE LIVER FAILURE.

Science.gov (United States)

Miguel, Fabiano Moraes; Schemitt, Elizângela Gonçalves; Colares, Josieli Raskopf; Hartmann, Renata Minuzzo; Morgan-Martins, Maria Isabel; Marroni, Norma Possa

2017-01-01

Severe Acute Liver Failure (ALF) is a life-threatening clinical syndrome characterized by hepatocyte necrosis, loss of hepatic architecture, and impairment of liver functions. One of the main causes of ALF is hepatotoxicity from chemical agents, which damage hepatocytes and result in increase of reactive oxygen species. The vitamin E isoform is the one with the strongest biological antioxidant activity. To evaluate the antioxidant effect of vitamin E in this ALF model. We used 56 rats (mean weight of 300 g) divided into eight groups, four groups assessed at 24 hours and 4 assessed at 48 hours after induction: control group (CO); Vitamin E (Vit. E); Thioacetamide (TAA) and Thioacetamide + Vitamina E (TAA+Vit.E). Rats were submitted to injections of thioacetamide (400 mg/kg i.p.) at baseline and 8 hours later. Vitamin E (100 mg/kg ip) was administered 30 minutes after the second dose of thioacetamide. The 48-hour group rats received two additional doses of vitamin E (24h and 36h). At 24h or 48 hours after the administration of the first dose of TAA, rats were weighed and anesthetized and their blood sampled for evaluation of liver integrity through enzymes aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Liver tissue was sampled for assessment of lipid peroxidation (LPO) by the technique TBARS, antioxidant enzymes SOD, CAT, GPx and GST activity, levels of the NO 2 /NO 3 and histology by H&E in two times. The results were expressed as mean ± standard deviation and statistically analyzed by ANOVA followed by Student-Newman-Keuls, with P E, we observed a reduction in liver enzymes AST (U/L) (101.32±19.45 in 24 hours and 97.85±29.65 in 48 hours) related to the TAA group (469.56± 0.69 in 24 hours and 598.23±55.45 in 48 hours) and ALT (U/L) (76.59±8.56 in 24 hours and 68.47±6.49 in 48 hours) compared to the TAA group (312.21±10.23 in 24 hours and 359.15±17.58 in 48 hours). There was a reduction of LPO (nmol/mg Prot) in the TAA+Vit.E group (0

Dicty_cDB: VSF233 [Dicty_cDB

Lifescience Database Archive (English)

Full Text Available lllks*vit*eimvikilisc*nqldlilrikknnnnnki*msl Frame B: ***sptrisctyc*kvr*llkr*wllry*ylvktn*i*f*elkkiiiiikfkch...y*nk*i kkk--- ---***sptrisctyc*kvr*llkr*wllry*ylvktn*i*f*elkkiiiiikfkch Frame C:

Correlation of Serum Magnesium with Serum Parathormone Levels in Patients on Regular Hemodialysis

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Baradaran Azar

2006-01-01

Full Text Available Secondary hyperparathyroidism (SHPT is a common, important, and treatable complication of end-stage renal disease. This study was conducted to investigate the role of serum magnesium (Mg in regulating the secretion of parathyroid hormone (PTH by the parathyroid gland in patients on maintenance hemodialysis (HD. Pre-dialysis serum levels of calcium (Ca, phosphorus (P, Mg, alkaline phosphatase (ALP, intact serum PTH (iPTH, serum 25-hydroxy Vitamin D (25-OH Vit D and plasma bicarbonate (HCO3 were measured. The Urea Reduction Rate as well as duration and dosage of HD treatment were noted. Our study did not show any significant correlation between serum Mg levels and duration of HD treatment, levels of serum ALP, and plasma HCO3, Ca and P. An inverse correlation, albeit insignificant, was found between the serum Mg levels and iPTH (r=-0.30 p=0.079; also, a significant positive correlation was found between serum Mg levels and serum 25-OH Vit D levels (r= 0.40 p= 0.009. Our findings are in agreement with previous data, which suggest that factors other than serum Mg are more important in the regulation of PTH secretion in HD patients. A positive and strong association between serum Mg with 25-OH Vit D needs to be studied in greater detail.

Photo-induced regeneration of hormones by electron transfer processes: Potential biological and medical consequences

Science.gov (United States)

Getoff, Nikola; Hartmann, Johannes; Schittl, Heike; Gerschpacher, Marion; Quint, Ruth Maria

2011-08-01

Based on the previous results concerning electron transfer processes in biological substances, it was of interest to investigate if hormone transients resulting by e.g. electron emission can be regenerated. The presented results prove for the first time that the hormone transients originating by the electron emission process can be successfully regenerated by the transfer of electrons from a potent electron donor, such as vitamin C (VitC). Investigations were performed using progesterone (PRG), testosterone (TES) and estrone (E1) as representatives of hormones. By irradiation with monochromatic UV light (λ=254 nm) in a media of 40% water and 60% ethanol, the degradation as well as the regeneration of the hormones was studied with each hormone individually and in the mixture with VitC as a function of the absorbed UV dose, using HPLC. Calculated from the obtained initial yields, the determined regeneration of PRG amounted to 52.7%, for TES to 58.6% and for E1 to 90.9%. The consumption of VitC was determined in the same way. The reported results concerning the regeneration of hormones by the transfer of electrons from an electron donor offer a new, promising method for the therapy with hormones. As a consequence of the regeneration of hormones, a decreased formation of carcinogenic metabolites is expected.

Photo-induced regeneration of hormones by electron transfer processes: Potential biological and medical consequences

Energy Technology Data Exchange (ETDEWEB)

Getoff, Nikola, E-mail: nikola.getoff@univie.ac.a [Section of Radiation Biology, Faculty of Life Sciences, University of Vienna, A-1090 Vienna (Austria); Hartmann, Johannes [Department of Gynecologic Endocrinology and Reproduction, Medical University of Vienna, A-1090 Vienna (Austria); Schittl, Heike [Section of Radiation Biology, Faculty of Life Sciences, University of Vienna, A-1090 Vienna (Austria); Gerschpacher, Marion [Department of Gynecologic Endocrinology and Reproduction, Medical University of Vienna, A-1090 Vienna (Austria); Quint, Ruth Maria [Section of Radiation Biology, Faculty of Life Sciences, University of Vienna, A-1090 Vienna (Austria)

2011-08-15

Based on the previous results concerning electron transfer processes in biological substances, it was of interest to investigate if hormone transients resulting by e.g. electron emission can be regenerated. The presented results prove for the first time that the hormone transients originating by the electron emission process can be successfully regenerated by the transfer of electrons from a potent electron donor, such as vitamin C (VitC). Investigations were performed using progesterone (PRG), testosterone (TES) and estrone (E1) as representatives of hormones. By irradiation with monochromatic UV light ({lambda}=254 nm) in a media of 40% water and 60% ethanol, the degradation as well as the regeneration of the hormones was studied with each hormone individually and in the mixture with VitC as a function of the absorbed UV dose, using HPLC. Calculated from the obtained initial yields, the determined regeneration of PRG amounted to 52.7%, for TES to 58.6% and for E1 to 90.9%. The consumption of VitC was determined in the same way. The reported results concerning the regeneration of hormones by the transfer of electrons from an electron donor offer a new, promising method for the therapy with hormones. As a consequence of the regeneration of hormones, a decreased formation of carcinogenic metabolites is expected.

Photo-induced regeneration of hormones by electron transfer processes: Potential biological and medical consequences

International Nuclear Information System (INIS)

Getoff, Nikola; Hartmann, Johannes; Schittl, Heike; Gerschpacher, Marion; Quint, Ruth Maria

2011-01-01

Based on the previous results concerning electron transfer processes in biological substances, it was of interest to investigate if hormone transients resulting by e.g. electron emission can be regenerated. The presented results prove for the first time that the hormone transients originating by the electron emission process can be successfully regenerated by the transfer of electrons from a potent electron donor, such as vitamin C (VitC). Investigations were performed using progesterone (PRG), testosterone (TES) and estrone (E1) as representatives of hormones. By irradiation with monochromatic UV light (λ=254 nm) in a media of 40% water and 60% ethanol, the degradation as well as the regeneration of the hormones was studied with each hormone individually and in the mixture with VitC as a function of the absorbed UV dose, using HPLC. Calculated from the obtained initial yields, the determined regeneration of PRG amounted to 52.7%, for TES to 58.6% and for E1 to 90.9%. The consumption of VitC was determined in the same way. The reported results concerning the regeneration of hormones by the transfer of electrons from an electron donor offer a new, promising method for the therapy with hormones. As a consequence of the regeneration of hormones, a decreased formation of carcinogenic metabolites is expected.

Volatile organic compounds speciation and their influence on ozone formation potential in an industrialized urban area in Brazil.

Science.gov (United States)

Galvão, Elson Silva; Santos, Jane Meri; Reis Junior, Neyval Costa; Stuetz, Richard Michael

2016-09-01

Speciation and the influence on the ozone formation potential (OFP) from volatile organic compounds (VOCs) have been studied between February June 2013 in Vitória, ES, Brazil. Passive samplers were installed at three air-quality monitoring stations and a total of 96 samplings were collected. A total of 78 VOCs were characterized by gas chromatograph-mass spectrometer. The predominant group was organic acids, followed by alcohols and substituted aromatics and 14 precursor species were quantified. An analysis correlating concentrations with wind direction was conducted to identify possible sources. The OFP was calculated applying the scale of maximum incremental reactivity proposed by Carter.[ 23 ] Ozone precursors with the greatest OFP such as undecane, toluene, ethylbenzene and m, p-xylene compounds were the most abundant with means of 0.855, 0.365, 0.259 and 0.289 µg m(-3), respectively. The benzene, toluene, ethylbenzene and xylene (BTEX) group was found below the limits considered harmful to the health of the population living in Vitória. The OFP calculated for the precursors group was 22.55 µg m(-3) for the rainy season and 32.11 µg m(-3) for the dry season. The VOC/NOx ratio in Vitória is approximately 1.71, indicating that the region has a VOC-limiting condition for the production of ozone.

The Effectivity of Green Coconut Water To Reduce Mercury Level In The Blood And To Improve Blood Profiles And Liver Cells Appearance (Study In Sprague Dawley Rats)

Science.gov (United States)

Abdulrzag, Ehmeeda M.; Nur Kristina, Tri; Suwondo, Ari; Sunoko, Henna Rya

2018-02-01

When people are exposed to mercury chloride, it can produce a variety of health effects in the blood and liver. Coconut water contains Zn, Fe, Vit. C, Vit B11, Vit. B6, and Se to reduce mercury chloride level in the blood and improve blood profile and liver cells. Aim of this study was to analysis the effect of green coconut water supplementation in overcoming the toxic effect of Hg chlorid in the blood and liver of Sprague dawley rats exposed to Hg chloride. Samples were randomly about 36 animals rats exposed to HgCl2 through forced feeding by 20 mg/kgBW sondage per day for 14 days, which divided into control group, and intervention groups were given fresh green coconut water in each by 6, 8, and 10 mL/kgBW for intervention 7 and 17 days. The result of this study showed that there is a significant effect and the decrease in mercury levels in the blood. There is no significant affect on the hemoglobin level, hematocrit level and platelet count with the treatment of green coconut water in the mice with exposure Hg. There is no significant effect between treatments using green coconut water with SGPT levels; there is a decrease in SGPT levels at the increasing number of doses of green coconut water and the length of treatment.

Journal of Chemical Sciences | Indian Academy of Sciences

Indian Academy of Sciences (India)

Home; Journals; Journal of Chemical Sciences; Volume 126; Issue 1 ... Centre for Nanotechnology Research, VIT University, Vellore 632 014, India; Department of ... Nissan Technology & Business Center India (P) Ltd., Chennai 603002, India ...

Acetylsalicylic acid supplementation improves protein utilization efficiency while vitamin E supplementation reduces markers of the inflammatory response in weaned pigs challenged with enterotoxigenic E.coli

Institute of Scientific and Technical Information of China (English)

Jae Cheol Kim; Bruce P.Mullan; John L.Black; Robert J.E.Hewitt; Robert J.van Barneveld; John R.Pluske

2017-01-01

Background:This experiment was conducted to test the hypothesis that vitamin E (Vit E) and acetylsalicylic acid (ASA),a cyclooxygenase-2 (COX-2) inhibitor,will additively reduce the production of the immunosuppressive molecule prostaglandin E2 (PGE2) and hence reduce inflammatory responses in weaner pigs experimentally infected with an enterotoxigenic strain of E.coli.Methods:The experiment was conducted in a research facility with 192 individually-housed male weaner pigs (Landrace × Large White) weighing 6.6 ± 0.04 kg (mean ± SEM).The pigs were experimentally infected with an enterotoxigenic strain of E.coli and were allocated to a 2 × 3 factorial design with the respective factors being without and with 125 ppm ASA and three levels ofVit E supplementation (50,100 or 200 IU/kg diet,dl-α-tocopheryl acetate).Results:Acetylsalicylic acid supplementation improved average daily gain (P ＜ 0.05) and tended to improve feed:gain ratio (P ＜ 0.10) during the first 14 d after weaning.Acetylsalicylic acid supplementation also improved (P ＜ 0.001) amino acid utilization efficiency (as assessed by plasma urea level) and tended to decrease (P ＜ 0.10) PGE2 production in the liver without affecting small intestinal histology and tight junction protein mRNA expression in the jejunal epithelium.Vitamin E supplementation greater than 100 IU/kg diet sustained both the plasma Vit E concentration (P ＜ 0.001) and plasma haptoglobin content (P ＜ 0.001) after weaning.However,there was no additive effects of the combined supplementation of ASA and Vit E on performance,intestinal barrier function and inflammatory responses of weaned pigs.Conclusions:Although ASA and vitamin E improved amino acid utilization efficiency and reduced acute inflammatory responses,ASA and vitamin E did not additively reduce production of PGE2 and inflammatory responses in weaner pigs experimentally infected with an enterotoxigenic strain of E.coli.

Effects of Pomegranate peel hydroAlcoholic extract and vitamin E supplementation on Paraoxonase, myeloperoxidase Activities and nitric oxide levels following an exhaustive exercise in rats

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Saeid Veiskarami

2017-03-01

Full Text Available Background: free radicals produced as a result of heavy training exercise especially oxygen species (ROS damage to body tissues Which can be prevent from this by consuming antioxidant substances timely. The aim of this study was to evaluate effect of Pomegranate (Punica Granatum peel hydro alcoholic on reduced of oxidative stress induced by an exhaustive exercise. Materials and Methods: Thirty two weight-matched male Wistar rats were evenly divided into: 1 control: received saline (0.2 ml saline/ rat by oral administration via epigastric tube. 2 Received oral administration of 200 mg/kg pomegranate peel hydro alcoholic extract (PPHE200. 3 Received oral administration of 250 mg/kg Pomegranate peel hydro alcoholic extract (PPHE250. 4 Received oral administration of vitamin E (vit E 5 mg/kg. Animals were submitted to swimming exhaustive exercise stress for an 8-week. At the end of the experiment, blood samples were collected for serum. Serum samples were analyzed for paraoxonase-1(PON-1 and myeloperoxidase (MPO activities and nitric oxide levels. Results: Paraoxonase-1 (PON-1 activities serum were significantly increases in PPHE200 (23.03±1.47, PPHE250 (23.59±1.98 and vit E (25.38±2.65 than in the control (18.57±1.380 (p<0.05.In PPHE200 (32.76±9.97 ،PPHE250 (31.45±6.05 and vit E (24.94±4.65 treated animals was determined in serum where myeloperoxidase activities reduced significantly compared with control (40.70±6.14 (p<0.05. Levels of Nitric oxide levels were significantly lower in PPHE 200 (46.59±2.48, PPHE250 (40.27±2.62 and vit E (36.25±3.82 treated than in control (47.18±5.36 (p<0.05. Conclusion: Results indicated that Pomegranate peel hydro alcoholic extract supplementations can strength antioxidant defense system and anti-inflammatory induced by exhaustive exercise.

Genetic polymorphism of cytochrome P450 4F2, vitamin E level and histological response in adults and children with nonalcoholic fatty liver disease who participated in PIVENS and TONIC clinical trials.

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Shaminie Athinarayanan

Full Text Available Vitamin E improved liver histology in children and adults with NAFLD who participated in TONIC and PIVENS clinical trials, but with significant inter-individual variability in its efficacy. Cytochrome P450 4F2 (CYP4F2 is the major enzyme metabolizing Vit E, with two common genetic variants (V433M, rs2108622 and W12G, rs3093105 found to alter its activity. We investigated the relationship between CYP4F2 genotypes, α-tocopherol levels and histological improvement in these two trials. V433M and W12G variants were genotyped in TONIC (n = 155 and PIVENS (n = 213 DNA samples. The relationships between CYP4F2 genotypes, plasma α-tocopherol levels at baseline and weeks 48 (w48 and 96 (w96 and histological end points (overall improvement in liver histology and resolution of NASH were investigated. As a result, the V433M genotype was significantly associated with baseline plasma α-tocopherol in the TONIC trial (p = 0.004, but not in PIVENS. Among those receiving Vit E treatment, CYP4F2 V433M genotype was associated with significantly decreased plasma α-tocopherol levels at w48 (p = 0.003 for PIVENS and p = 0.026 for TONIC but not at w96. The w96 α-tocopherol level was significantly associated with resolution of NASH (p = 0.006 and overall histology improvement (p = 0.021in the PIVENS, but not in the TONIC trial. There was no significant association between CYP4F2 genotypes and histological end points in either trial. Our study suggested the a moderate role of CYP4F2 polymorphisms in affecting the pharmacokinetics of Vit E as a therapeutic agent. In addition, there may be age-dependent relationship between CYP4F2 genetic variability and Vit E pharmacokinetics in NAFLD.

Vitamina C restaura pressão arterial e a resposta vasodilatadora no antebraço em crianças obesas

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Pricilla Regina Oliveira Fernandes Fernandes

2011-06-01

Full Text Available FUNDAMENTO: A resposta vasodilatadora periférica tem um papel importante na fisiopatologia da obesidade e das doenças cardíacas. OBJETIVO: Avaliar o efeito crônico da suplementação de vitamina C (VitC sobre a pressão arterial e na resposta vasodilatadora ao estresse mental. MÉTODOS: Neste estudo prospectivo, randomizado e duplo cego foram avaliadas crianças obesas, de ambos os gêneros e com idade entre 8 a 12 anos divididas em 2 grupos: 1 grupo de crianças suplementadas com 500 mg de vitamina C (n = 11 e, 2 substância placebo (n = 10 durante 45 dias. Oito crianças eutróficas, pareadas por idade também foram arroladas no estudo. Foi avaliada a pressão arterial média (PAM, frequência cardíaca (ECG e fluxo sanguíneo no antebraço pela plestimografia de oclusão venosa. A condutância vascular no antebraço (CVA foi obtida através da relação entre o fluxo sanguíneo no antebraço e a PAM (X100. RESULTADOS: Antes da intervenção, as crianças obesas apresentaram PAM maior e CVA menor quando comparadas às crianças eutróficas. Pós-intervenção, o Grupo VitC apresentou redução da PAM no repouso (81 ± 2 vs 75 ± 1 mmHg, p = 0,01, enquanto no Grupo Placebo não houve alteração da PAM (p = 0,58. Adicionalmente, VitC promoveu um aumento da CVA no repouso (3,40 ± 0,5 vs 5,09 ± 0,6 un, p = 0,04 e durante o estresse mental (3,92 ± 0,5 vs 6,68 ± 0,9 un, p = 0,03. Além disso, pós suplementação com VitC, os níveis da CVA foram estatisticamente semelhantes aos das crianças eutróficas no repouso (5,09 ± 0,6 vs 5,82 ± 0,4 un, p > 0,05 e durante o estresse mental (6,68 ± 0,9 vs 7,35 ± 0,5 un, p > 0,05. CONCLUSÃO: Suplementação com VitC reduziu a pressão arterial e restabeleceu a resposta vasodilatadora periférica em crianças obesas.

Assay of 25-OH vitamin D3

International Nuclear Information System (INIS)

Nayer, P. de; Thalasso, M.; Beckers, C.

1977-01-01

A simplified version of the competitive protein binding assay for 25-OH vit D3 derived from the method of Belsey et al. is presented. The procedure does not include a chromatography step, and is performed on an alcoolic extract of 0.1 ml plasma or serum. Normal rat serum (1:20,000) was used as binding protein. No β-lipoproteins were added to the assay buffer. A 10% displacement of the tracer was observed at 0.04 ng/tube, and 50% at 0.15 ng/tube, allowing for the measurement of 25-OH vit D3 concentrations between 2 ng/ml and 200 ng/ml. Mean values in a normal group was 23.1 +- 6.5 ng/ml (range 16-37 ng/ml, n = 11). (orig.) [de

Research Note Development and characterization of microsatellite ...

Indian Academy of Sciences (India)

Srirama R

2School of Biosciences and Technology, VIT University, Vellore 632014, India. 7. 8 ... microsatellite markers developed can be used for studying the population ... characterized by diverse growth forms, including shrubs, trees, and annual or ...

A novel microbond bundle pullout technique to evaluate the ...

Indian Academy of Sciences (India)

2017-07-26

Jul 26, 2017 ... Department of Manufacturing, School of Mechanical Engineering, VIT-University, Vellore 632014, India .... The mean and the standard deviation values for the droplets and force plots are ..... tolerance, to mention a few. 5.

COLOR POLLUTION CONTROL IN TEXTILE DYEING INDUSTRY ...

African Journals Online (AJOL)

a

tannery sludge derived activated carbon is lower compared with commercial ... industrial solid waste for the treatment of wastewater is a win-win strategy ..... The authors are thankful to the management of VIT University, Vellore, India for ...

Evaluation of serum Vitamin B12 level and related nutritional status ...

African Journals Online (AJOL)

2016-03-29

Mar 29, 2016 ... This study was aimed to investigate the association between serum. Vitamin B12 ... Consumption of certain types of food contributes to increase vitB12 level. Key words: ... nutritional status among apparently healthy obese.

Studies on cutaneous lipid peroxide with special reference to the influences of ultraviolet irradiation

International Nuclear Information System (INIS)

Nomura, Kazuo

1981-01-01

The purpose of this study was to investigate the participation of lipid peroxide (LP) in some skin damages due to ultraviolet (UV) irradiation. Results obtained were as follows. 1) Long wave UV (UVA) was irradiated to rat skin homogenates. The levels of LP increased linearly with irradiation time. 2) When 8-methoxypsoralen was added to the homogenates prior to UVA irradiation, however, the LP levels showed no increase. 3) Various anti-oxidative agents were added to homogenates and UVA was irradiated. Only Vit. E reduced the LP levels in proportion to its concentrations. 4) Anti-oxidative agents were given to rats which were then exposed to PUVA (8-methoxypsoralen plus UVA) treatment. Among them, administration of Vit. E and pantethine was associated with reduction of serum and cutaneous LP levels with only slight histologic changes in the involved skin. 5) Vit. E deficient rats were treated with PUVA. In these models, cutaneous LP levels raised from 24 hours to 96 hours after PUVA treatment and histologic changes such as vacuolization, blister formation and cell degeneration were remarkable. From the above data, it became evident that lipid peroxidation took place in skin tissue per se and even in the UVA wave length region. After PUVA treatment, cutaneous LP levels relatively well correlated with histologic changes of the involved skin. The results suggested that LP played a certain role in skin damages due to UV. (author)

Protective effects of vitamin E on cyclosporineA-induced toxicity in rat testis

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Hamidreza Sameni

2011-07-01

Full Text Available Introduction: Cyclosporine A (CsA as an immunosuppressive drug which widely used in organ transplantation and autoimmune diseases. This drug is caused many injuries and cell cytotoxic of the body organs such as reproductive organs. The aim of this study was to investigate the possible protective effects of vitamin E (Vit E against CsA-induced damages in rat testis. Material and Methods: 40 adult male wistar rats were divided into 5 groups: control (without any intervention, placebo (received only pure olive oil, test 1 (CsA+olive oil, 30 mg/kg, test 2 (Vit E, 100 mg/kg and test 3 (CsA+Vit E, with the same dose. All animal received drugs for three weeks daily by oral gavages. Following, the testis were fixed and sections stained with Haematoxylin & Eosin and Trichrome Masson. Then with using a microscope equipped with a scaled ocular micrometer and image analysis software were histomorphometry. Results: This study showed that CsA caused severe degenerative changes in testicular tissue include decreased seminiferous tubules diameter, seminiferous epithelium thickness. Also, the number of spermatogonia, primary spermatocyte, spermatozoa, and sertoli and leydig cells significantly decreased throughout the experiment. These changes are lead to turbulence and atrophy seminiferous epithelium and delay in spermatogenesis. Treatment with vitamin E minimized the adverse effects of CsA on testis structure and spermatogenesis. Conclusion: These results suggest that vitamin E has a protective effect against CsA-induced testicular toxicity in male rat.

MODIS-derived EVI, NDVI and WDRVI time series to estimate phenological metrics in French deciduous forests

Science.gov (United States)

Testa, S.; Soudani, K.; Boschetti, L.; Borgogno Mondino, E.

2018-02-01

Monitoring forest phenology allows us to study the effects of climate change on vegetated land surfaces. Daily and composite time series (TS) of several vegetation indices (VIs) from MODerate resolution Imaging Spectroradiometer (MODIS) data have been widely used in scientific works for phenological studies since the beginning of the MODIS mission. The objective of this work was to use MODIS data to find the best VI/TS combination to estimate start-of-season (SOS) and end-of-season (EOS) dates across 50 temperate deciduous forests. Our research used as inputs 2001-2012 daily reflectance from MOD09GQ/MOD09GA products and 16-day composite VIs from the MOD13Q1 dataset. The 50 pixels centered on the 50 forest plots were extracted from the above-mentioned MODIS imagery; we then generated 5 different types of TS (1 daily from MOD09 and 4 composite from MOD13Q1) and used all of them to implement 6 VIs, obtaining 30 VI/TS combinations. SOS and EOS estimates were determined for each pixel/year and each VI/TS combination. SOS/EOS estimations were then validated against ground phenological observations. Results showed that, in our test areas, composite TS, if actual acquisition date is considered, performed mostly better than daily TS. EVI, WDRVI0.20 and NDVI were more suitable to SOS estimation, while WDRVI0.05 and EVI were more convenient in estimating early and advanced EOS, respectively.

The role of nitrergic system in antidepressant effects of acute administration of zinc, magnesium and thiamine on progesterone induced postpartum depression in mice

Directory of Open Access Journals (Sweden)

Nikseresht S

2010-08-01

Full Text Available "nBackground: Postpartum depression is a mood disorder that has harmful effects on mothers, infants, family and relationships. Acute decrease of progesterone after delivery has been proposed as a cause for postpartum depression. This hormone can affect neurotransmitters' function. Zinc (Zn and magnesium (Mg as trace elements exert their antidepressant effects through neurotransmitter pathways. On the other hand, thiamin (Vit B1 deficiency leads to depression in animal models. The aim of this study was to evaluate effects of combination of zinc, magnesium and thiamine on postpartum depression and role of nitrergic system. "n"nMethods: One hundred ten female mice in five groups were used. Postpartum depression was conducted using progesterone injections. Combinations of Zinc chloride, magnesium chloride and thiamine HCL were administered 30 minutes before open field and forced swimming test (FST. In order to investigate role of nitrergic system, L-arginine and LNAME were administered. "n"nResults: All treatment groups spent less immobility time than the control group (p< 0.05. Combined administration of Zn+ Mg+ Vit B1 caused the most reduction in immobility time. Administration of L-NAME in Zn+ Mg+ Vit B1 group caused reduction in immobility time while administration of L-arginine caused increase in immobility time in the same group. "nConclusion: Zinc, magnesium and thiamine can improve depressive symptoms by nitrergic pathway. These elements as supplement compounds could be alternatives for antidepressants in postpartum period.

Browse Title Index

African Journals Online (AJOL)

... inside switchyard substation (case study: El-Hadjar electrical post in Annaba city, ... in uniformly heated vertical porous coated tubes at low pressure, Abstract PDF ... Vol 16 (2007), Resistance, fiabilité et longévité de plastiques renforces ...

Bio-synthesis of silver nanoparticles using agroforestry residue and their catalytic degradation for sustainable waste management

CSIR Research Space (South Africa)

Anand, K

2017-07-01

Full Text Available , South Africa 4 Department of Textile Science, Faculty of Science, Nelson Mandela Metropolitan University, P.O. Box 77000, Port Elizabeth 6031, South Africa 5 Department of Chemistry, SAS, VIT University, Vellore, Tamil Nadu 632014, India Abstract...

Modern human sperm freezing: Effect on DNA, chromatin and acrosome integrity

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Tahereh Rahiminia

2017-08-01

Conclusion: Sperm in Vapour was healthier in terms of DNA, chromatin and acrosome integrity. In contrast of higher motility and normal morphology; DNA, chromatin and acrosome integrity were decreased in Vit. However, these findings were more acceptable in SSV or Vapour.

Feeding the insect industry

Science.gov (United States)

This article reports the use of insect colloidal artificial diets suitable for the rearing of economically important arthropods, such as Lygus lineolaris, Lygus hesperus, Coleomegilla maculata, and Phytoseiulus persimilis The different diets contain key nutrients such as proteins, carbohydrates, vit...

Bulletin of Materials Science | Indian Academy of Sciences

Indian Academy of Sciences (India)

Author Affiliations. Tanushree Choudhury1 Nirendra M Misra2. Department of Chemistry, School of Advanced Sciences, VIT University Chennai Campus, Vandalur- Kelambakkam Road, Chennai- 600048, India; Department of Applied Chemistry, Indian School of Mines University, Dhanbad 826 004, India ...

The effect of different cage tiers and age on egg performance, egg ...

African Journals Online (AJOL)

admOUjg

2014-10-04

Oct 4, 2014 ... ... and health benefits (Appleby, 1998; Rodenburg et al., 2005; Vits et al., 2005). .... All hens stayed healthy over the experimental period. No birds were ..... Effects of housing system (outdoor vs cages) and age of laying hens ...

About the Eye

Medline Plus

Full Text Available ... the white outer coating of the eye. Vitreous humor (VIT-ree-us HYOO-mer) is the clear ... in Spanish (Información en español) Website, Social Media Policies and Other Important Links NEI Employee Emergency Information ...

Väga kuldne trio / Ilona Martson

Index Scriptorium Estoniae

Martson, Ilona, 1970-

2008-01-01

Leonid Gaidai 60ndatel loodud filmid "Koer Barboss ja ebatavaline kross", "Kaukaasia vang", "Operatsioon Õ", kus osaleb kuulus vene koomikute trio Nikulin-Vitsõn-Morgunov. Režissöörist, näitlejatest ja nende filmide sünniloost

A potência instituinte no projeto "Exercendo Cidadania" La potencia instituyente en el proyecto "Ejerciendo la Ciudadanía" The instituting power in the project "Exercising Citizenship"

Directory of Open Access Journals (Sweden)

Fabiana Davel Canal

2012-12-01

Full Text Available As Penas e Medidas Alternativas (PMAs são cada vez mais utilizadas pelo sistema jurídico brasileiro. Diante desse contexto, a Vara de Execuções de Penas e Medidas Alternativas do Espírito Santo estabeleceu um convênio com Prefeitura Municipal de Vitória que oferece um curso de formação em Direitos Humanos, intitulado "Exercendo Cidadania" como forma de cumprimento das PMAs. Este artigo é resultado da análise preliminar de uma pesquisa que tem como objetivo analisar a execução das PMAs no município de Vitória-ES, com atenção especial para o curso em questão. O diário de campo foi o principal instrumento metodológico, confeccionado pelas vivências no curso de outubro a novembro de 2010. Ficamos atentos aos movimentos que nos indicavam como o apenado entende/vive/pensa a PMA,observando as formas rígidas, instituídas de efetivação das mesmas, mas também - e isso que tentamos dar visibilidade - a potência instituinte do curso.Las Penas y Medidas Alternativas (PMAs son cada vez más utilizadas por el sistema jurídico brasileño. En este contexto, el Tribunal de Ejecución de Penas y Medidas Alternativas del Espírito Santo ha establecido un acuerdo con el Ayuntamiento de la ciudad de Vitória, que ofrece un curso de capacitación sobre Derechos Humanos titulado "Ejerciendo la Ciudadanía" como una manera de cumplir con las PMAs. Este artículo es el resultado de un análisis preliminar de una investigación que tiene como objetivo analizar el desempeño de las PMAs en la ciudad de Vitória, con especial atención al curso en cuestión. El diario de campo fue la principal herramienta metodológica, hecho a partir de experiencias en el curso de octubre a noviembre de 2010. Nos pusimos atentos a los movimientos que nos indicaban cómo el apenado entiende/vive/piensa la PMA, observando las formas rígidas, instituidas de realización de las mismas, pero también - y fue a eso que tratamos de dar visibilidad - la potencia

Risco cardiovascular em vegetarianos e onívoros: um estudo comparativo Cardiovascular risk in vegetarians and omnivores: a comparative study

Directory of Open Access Journals (Sweden)

Rita de Cássia Moreira de Almeida Teixeira

2007-10-01

Full Text Available FUNDAMENTO: Estudos clínicos e epidemiológicos demonstram grande associação da dieta com os agravos crônicos, particularmente com os eventos cardiovasculares, apesar de ainda não compreendidos todos os seus mecanismos de ação. OBJETIVO: Descrever e analisar o risco cardiovascular em vegetarianos e onívoros residentes na Grande Vitória/ES, na faixa etária de 35 a 64 anos. MÉTODOS: Para avaliação do risco cardiovascular foi realizado estudo de coorte histórico com 201 indivíduos. Foram incluídos 67 vegetarianos há no mínimo 5 anos, provenientes da Grande Vitória, e 134 onívoros, participantes do Projeto MONICA/Vitória, pareados por classe socioeconômica, sexo, idade e raça. Medidas bioquímicas e hemodinâmicas foram obtidas na Clínica de Investigação Cardiovascular da UFES. Para comparação de proporções, foi usado o teste chi2 e calculada a razão de prevalência. O risco cardiovascular foi calculado por meio do algoritmo de Framingham. RESULTADOS: A idade média do grupo foi de 47 ± 8 anos e o tempo médio de vegetarianismo 19 ± 10 anos, sendo a dieta ovolactovegetariana seguida por 73% dos vegetarianos. Pressão arterial, glicemia de jejum, colesterol total, colesterol de lipoproteína de baixa densidade (LDL-colesterol e triglicerídeos foram mais baixos entre vegetarianos (pBACKGROUND: Clinical and epidemiological studies have demonstrated a strong association between eating habits and chronic diseases, particularly cardiovascular events, although not all the mechanisms of action are understood. OBJECTIVE: To describe and analyze the cardiovascular risk (CVR in vegetarians and omnivores residing in Greater Vitória, State of Espírito Santo, Brazil, in the age range from 35 to 64 years. METHODS: To evaluate CVR in the groups, a historical cohort study with 201 individuals was conducted. Sixty seven individuals who had been following a vegetarian diet for at least five years, and who were from Greater Vit

A novel multifunctional biomedical material based on polyacrylonitrile: Preparation and characterization

Energy Technology Data Exchange (ETDEWEB)

Wu, Huan-ling [College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620 (China); Jiuzhou College of Pharmacy, Yancheng Institute of Industry Technology, Yancheng 224005 (China); Bremner, David H. [School of Science, Engineering and Technology, Kydd Building, Abertay University, Dundee DD1 1HG, Scotland (United Kingdom); Li, He-yu; Shi, Qi-quan; Wu, Jun-zi; Xiao, Rui-qiu [College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620 (China); Zhu, Li-min, E-mail: lzhu@dhu.edu.cn [College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620 (China)

2016-05-01

Wet spun microfibers have great potential in the design of multifunctional controlled release materials. Curcumin (Cur) and vitamin E acetate (Vit. E Ac) were used as a model drug system to evaluate the potential application of the drug-loaded microfiber system for enhanced delivery. The drugs and polyacrylonitrile (PAN) were blended together and spun to produce the target drug-loaded microfiber using an improved wet-spinning method and then the microfibers were successfully woven into fabrics. Morphological, mechanical properties, thermal behavior, drug release performance characteristics, and cytocompatibility were determined. The drug-loaded microfiber had a lobed “kidney” shape with a height of 50–100 μm and width of 100–200 μm. The addition of Cur and Vit. E Ac had a great influence on the surface and cross section structure of the microfiber, leading to a rough surface having microvoids. X-ray diffraction and Fourier transform infrared spectroscopy indicated that the drugs were successfully encapsulated and dispersed evenly in the microfilament fiber. After drug loading, the mechanical performance of the microfilament changed, with the breaking strength improved slightly, but the tensile elongation increased significantly. Thermogravimetric results showed that the drug load had no apparent adverse effect on the thermal properties of the microfibers. However, drug release from the fiber, as determined through in-vitro experiments, is relatively low and this property is maintained over time. Furthermore, in-vitro cytocompatibility testing showed that no cytotoxicity on the L929 cells was found up to 5% and 10% respectively of the theoretical drug loading content (TDLC) of curcumin and vitamin E acetate. This study provides reference data to aid the development of multifunctional textiles and to explore their use in the biomedical material field. - Highlights: • Based on a wet spinning technique, a series of filaments which can be used as biomaterial

Functional traits of selected mangrove species in Brazil as biological indicators of different environmental conditions

Energy Technology Data Exchange (ETDEWEB)

Arrivabene, Hiulana Pereira [Universidade Federal do Espírito Santo, Centro de Ciências Humanas e Naturais, Departamento de Ciências Biológicas, 29075-910 Vitória, Espírito Santo (Brazil); Souza, Iara [Universidade Federal de São Carlos, Centro de Ciências Biológicas e da Saúde, Departamento de Ciências Fisiológicas, 13565-905 São Carlos (Brazil); Có, Walter Luiz Oliveira [Associação Educational de Vitória, Departamento de Biologia, 29053-360 Vitória (Brazil); Rodella, Roberto Antônio [Universidade Estadual Paulista Júlio de Mesquita Filho, Campus de Botucatu, Instituto de Biociências, Departamento de Botânica, C. Postal 510, 18618-000 Botucatu, São Paulo (Brazil); Wunderlin, Daniel Alberto, E-mail: dwunder@fcq.unc.edu.ar [Instituto de Ciencia y Tecnología de Alimentos Córdoba (ICYTAC), CONICET, Dpto. Qca. Orgánica, Fac. Cs. Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000, Córdoba (Argentina); and others

2014-04-01

Ecological studies on phenotypic plasticity illustrate the relevance of this phenomenon in nature. Conditions of biota reflect environmental changes, highlighting the adaptability of resident species that can be used as bioindicators of such changes. We report the morpho-anatomical plasticity of leaves of Avicennia schaueriana Stapf and Leechm. ex Moldenke, Laguncularia racemosa (L.) C.F.Gaertn. and Rhizophora mangle L., evaluated in three estuaries (Vitória bay, Santa Cruz and Itaúnas River; state of Espírito Santo, Brazil), considering five areas of mangrove ecosystems with diverse environmental issues. Two sampling sites are part of the Ecological Station Lameirão Island in Vitória bay, close to a harbor. A third sampling site in Cariacica (Vitória bay) is inside the Vitória harbor and also is influenced by domestic sewage. The fourth studied area (Santa Cruz) is part of Piraquê Mangrove Ecological Reservation, while the fifth (Itaúnas River) is a small mangrove, with sandy sediment and greater photosynthetically active radiation, also not strongly influenced by anthropic activity. Results pointed out the morpho-anatomical plasticity in studied species, showing that A. schaueriana and L. racemosa might be considered the most appropriate bioindicators to indicate different settings and environmental conditions. Particularly, the dry mass per leaf area (LMA) of A. schaueriana was the main biomarker measured. In our study, LMA of A. schaueriana was positively correlated with salinity (Spearman 0.71), Mn content (0.81) and pH (0.82) but negatively correlated with phosphorus content (− 0.63). Thus, the evaluation of modification in LMA of A. schaueriana pointed out changes among five studied sites, suggesting its use to reflect changes in the environment, which could be also useful in the future to evaluate the climate change. - Highlights: • We investigated adaptive modifications in plants in response to differences among three estuaries. • We used

Functional traits of selected mangrove species in Brazil as biological indicators of different environmental conditions

International Nuclear Information System (INIS)

Arrivabene, Hiulana Pereira; Souza, Iara; Có, Walter Luiz Oliveira; Rodella, Roberto Antônio; Wunderlin, Daniel Alberto

2014-01-01

Ecological studies on phenotypic plasticity illustrate the relevance of this phenomenon in nature. Conditions of biota reflect environmental changes, highlighting the adaptability of resident species that can be used as bioindicators of such changes. We report the morpho-anatomical plasticity of leaves of Avicennia schaueriana Stapf and Leechm. ex Moldenke, Laguncularia racemosa (L.) C.F.Gaertn. and Rhizophora mangle L., evaluated in three estuaries (Vitória bay, Santa Cruz and Itaúnas River; state of Espírito Santo, Brazil), considering five areas of mangrove ecosystems with diverse environmental issues. Two sampling sites are part of the Ecological Station Lameirão Island in Vitória bay, close to a harbor. A third sampling site in Cariacica (Vitória bay) is inside the Vitória harbor and also is influenced by domestic sewage. The fourth studied area (Santa Cruz) is part of Piraquê Mangrove Ecological Reservation, while the fifth (Itaúnas River) is a small mangrove, with sandy sediment and greater photosynthetically active radiation, also not strongly influenced by anthropic activity. Results pointed out the morpho-anatomical plasticity in studied species, showing that A. schaueriana and L. racemosa might be considered the most appropriate bioindicators to indicate different settings and environmental conditions. Particularly, the dry mass per leaf area (LMA) of A. schaueriana was the main biomarker measured. In our study, LMA of A. schaueriana was positively correlated with salinity (Spearman 0.71), Mn content (0.81) and pH (0.82) but negatively correlated with phosphorus content (− 0.63). Thus, the evaluation of modification in LMA of A. schaueriana pointed out changes among five studied sites, suggesting its use to reflect changes in the environment, which could be also useful in the future to evaluate the climate change. - Highlights: • We investigated adaptive modifications in plants in response to differences among three estuaries. • We used

A novel multifunctional biomedical material based on polyacrylonitrile: Preparation and characterization

International Nuclear Information System (INIS)

Wu, Huan-ling; Bremner, David H.; Li, He-yu; Shi, Qi-quan; Wu, Jun-zi; Xiao, Rui-qiu; Zhu, Li-min

2016-01-01

Wet spun microfibers have great potential in the design of multifunctional controlled release materials. Curcumin (Cur) and vitamin E acetate (Vit. E Ac) were used as a model drug system to evaluate the potential application of the drug-loaded microfiber system for enhanced delivery. The drugs and polyacrylonitrile (PAN) were blended together and spun to produce the target drug-loaded microfiber using an improved wet-spinning method and then the microfibers were successfully woven into fabrics. Morphological, mechanical properties, thermal behavior, drug release performance characteristics, and cytocompatibility were determined. The drug-loaded microfiber had a lobed “kidney” shape with a height of 50–100 μm and width of 100–200 μm. The addition of Cur and Vit. E Ac had a great influence on the surface and cross section structure of the microfiber, leading to a rough surface having microvoids. X-ray diffraction and Fourier transform infrared spectroscopy indicated that the drugs were successfully encapsulated and dispersed evenly in the microfilament fiber. After drug loading, the mechanical performance of the microfilament changed, with the breaking strength improved slightly, but the tensile elongation increased significantly. Thermogravimetric results showed that the drug load had no apparent adverse effect on the thermal properties of the microfibers. However, drug release from the fiber, as determined through in-vitro experiments, is relatively low and this property is maintained over time. Furthermore, in-vitro cytocompatibility testing showed that no cytotoxicity on the L929 cells was found up to 5% and 10% respectively of the theoretical drug loading content (TDLC) of curcumin and vitamin E acetate. This study provides reference data to aid the development of multifunctional textiles and to explore their use in the biomedical material field. - Highlights: • Based on a wet spinning technique, a series of filaments which can be used as biomaterial

Sustained Increase of 25-Hydroxyvitamin D Levels in Healthy Young Women during Wintertime after Three Suberythemal UV Irradiations-The MUVY Pilot Study.

Directory of Open Access Journals (Sweden)

Maria Gudrun Biersack

Full Text Available Vitamin D (VitD deficiency is a health problem prevalent not only in the elderly but also in young adults. The primary objective of our observational pilot study "MUVY" (Mood, UVR, Vitamin D in Young women was to test both the short-term and long-term effects of a series of three suberythemal UV radiation (UVR exposures on the VitD status and well-being of young healthy women during winter in a repeat measure design.20 healthy young women (Fitzpatrick skin types I-III, aged 21-25 years received three full body broad band UVR exposures with an escalating erythemally weighted dose schedule during one week in winter, and completed self-report questionnaires monitoring symptoms of depression (Beck Depression Inventory, BDI and affective state/well-being (Profile of Mood States, POMS at baseline and three days after the last UVR exposure. 25-hydroxyvitamin D (25(OHD and 1,25-dihydroxyvitamin D (1,25(OH2D were measured in serum at baseline, and at study days 8, 36 and 50.Mean baseline 25(OHD level was 54.3 nmol/L (standard deviation (s.d. = 24.1, with seven women having VitD deficient status. Relevant symptoms of depression, as indicated by low BDI total scores (0-8, were absent. After the three UVR exposures the increment of 25(OHD was an average of 13.9 nmol/L (95% confidence interval (CI = 9.4-18.4 and 26.2 pmol/L (95%CI = 7.2-45.1 for 1,25(OH2D. Δ25(OHD, and corresponding baseline levels were significantly and inversely associated (rho = -0.493, p = 0.027. Only 25(OHD remained significantly increased above baseline for at least six weeks after the last UVR exposure. A strong inverse correlation of the POMS subscale "Vigor/Activity" and the increment in 1,25(OH2D was found (rho = -0.739, p<0.001 at day 8.Three suberythemal whole body UVR exposures during one week are a simple and suitable method for improving 25(OHD levels during winter, for at least six weeks, and especially in young women with VitD deficient status.German Clinical Trials

Correlation of methylenetetrahydrofolate reductase polymorphism with Hcy metabolism and inflammatory response in patients with recurrent cerebral infarction

Directory of Open Access Journals (Sweden)

Gai-Zhuang Liu

2017-07-01

Full Text Available Objective: To study the correlation of methylenetetrahydrofolate reductase (MTHFR polymorphism with Hcy metabolism and inflammatory response in patients with recurrent cerebral infarction. Methods: 40 patients with recurrent cerebral infarction who were treated in Yulin Third Hospital between December 2013 and December 2016 were selected as recurrent group, 58 patients with primary cerebral infarction were selected as primary group, and 60 healthy volunteers were selected as control group. Peripheral blood MTHFR gene C677T polymorphism and serum levels of Hcy metabolism indexes and inflammatory response indicators were determined. Results: CC genotype constituent ratio of recurrent group was significantly lower than that of primary group and control group while CT genotype and TT genotype constituent ratio were significantly higher than those of primary group and control group; serum Hcy, HMGB1, sCD40L, YKL-40, Lp-PLA2 and MMP-9 levels in recurrent group and primary group were significantly higher than those in control group while VitB12 and FA levels were significantly lower than those in control group; serum Hcy, HMGB1, sCD40L, YKL-40, Lp-PLA2 and MMP-9 levels in recurrent group were significantly higher than those in primary group while VitB12 and FA levels were significantly lower than those in primary group. Serum Hcy, HMGB1, sCD40L, YKL-40, Lp-PLA2 and MMP-9 levels in patients with CC genotype were significantly lower than those in patients with CT genotype and TT genotype while VitB12 and FA levels were significantly higher than those in patients with CT genotype and TT genotype; serum Hcy, HMGB1, sCD40L, YKL-40, Lp-PLA2 and MMP-9 levels in patients with CT genotype were significantly lower than those in patients with TT genotype while VitB12 and FA levels were significantly higher than those in patients with TT genotype. Conclusion: MTHFR gene C677T polymorphism is closely related to the recurrence of cerebral infarction, and allele C

Desempenho de cultivares de alface em cultivo hidropônico sob dois níveis de condutividade elétrica Evaluation of two electric conductivity levels on the hidroponic cultivation of lettuce

Directory of Open Access Journals (Sweden)

Adriana G Magalhães

2010-09-01

Full Text Available O objetivo do trabalho foi comparar cultivares de alface de folhas soltas lisas, submetidas a estresse osmótico em soluções nutritivas sob cultivo hidropônico. Foram realizados dois experimentos, cada um com uma condutividade elétrica (C.E. de 2,0 dS m-1 e 2,5 dS m-1. Os tratamentos foram as cultivares de alface Babá de Verão, Floresta, Luisa, Manoa, Regina 579, Saia Véia e Vitória Verdinha. O delineamento experimental foi blocos casualizados, com três repetições. A colheita foi feita aos 45 dias após a semeadura. Nenhuma cultivar se destacou no conjunto das características avaliadas, embora a Manoa tenha apresentado o maior índice de queima de bordas das folhas, sem diferir estatisticamente da Saia Véia e Babá de Verão. As cultivares Regina 579 e Vitória Verdinha são as preferidas dos produtores hidropônico de Pernambuco, devido à falta de genótipos mais adaptados a essa modalidade de cultivo, e por apresentarem folhas lisas, levemente enrugadas e de textura macia.The aim of this work was to compare loose-leaf lettuce cultivars submitted to osmotic stress in nutrient solutions in hydroponic culture. Two experiments were carried out, with electric conductivity of 2.0 or 2.5 dS m-1. The treatments were the cultivars Babá de Verão, Floresta, Luisa, Manoa, Regina 579, Saia Véia, and Vitória Verdinha. The experimental design was randomized blocks with three replications. Harvest was performed 45 days after sowing. None among the cultivars differed clearly on the characteristics evaluated. However, "Manoa" showed the highest rate of leaf-edge burning, without significant difference in comparison to "Saia Véia" and "Babá de Verão" for summer. The cultivars Regina 579 and Vitória Verdinha are preferred by the growers of hydroponic vegetables of Pernambuco, due to the lack of better genotypes for this type of cultivation and due to the presence of smooth slightly-wrinkled leaves with soft texture.

Mastocytosis and insect venom allergy : diagnosis, safety and efficacy of venom immunotherapy

NARCIS (Netherlands)

Niedoszytko, M.; de Monchy, J.; van Doormaal, J. J.; Jassem, E.; Oude Elberink, J. N. G.

The most important causative factor for anaphylaxis in mastocytosis are insect stings. The purpose of this review is to analyse the available data concerning prevalence, diagnosis, safety and effectiveness of venom immunotherapy (VIT) in mastocytosis patients. If data were unclear, authors were

Machine Learning Approaches for Predicting Human Skin Sensitization Hazard

Science.gov (United States)

One of ICCVAM’s top priorities is the development and evaluation of non-animal approaches to identify potential skin sensitizers. The complexity of biological events necessary for a substance to elicit a skin sensitization reaction suggests that no single in chemico, in vit...

Transcriptome-based identification of pro- and antioxidative gene expression in kidney cortex of nitric oxide-depleted rats

NARCIS (Netherlands)

Wesseling, Sebastiaan; Joles, Jaap A.; van Goor, Harry; Bluyssen, Hans A.; Kemmeren, Patrick; Holstege, Frank C.; Koomans, Hein A.; Braam, Branko

2007-01-01

Nitric oxide (NO) depletion in rats induces severe endothelial dysfunction within 4 days. Subsequently, hypertension and renal injury develop, which are ameliorated by alpha-tocopherol (VitE) cotreatment. The hypothesis of the present study was that NO synthase (NOS) inhibition induces a renal

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African Journals Online (AJOL)

DR ADESOPE

Calorimeter determination of phosphorus in soil and plant materials ... Vit: A Pfizer livestock feeds production supplying the following vitamins and minerals per gram of diet: A, 9823 I.U; D3, 1965 I.U.; B12, 10 g/ton; Riboflavin, 10mg; ...

Studies on Cr(VI) removal from aqueous solutions by nanotitania ...

Indian Academy of Sciences (India)

applied for waste water treatment like zinc oxide and iron oxide to name a few.12 .... Here, qe is the metal concentration present on solid nano- titania, Ce the metal ..... We thank the management of VIT University for their support in research.

Pegvisomant-induced serum insulin-like growth factor-I normalization in patients with acromegaly returns elevated markers of bone turnover to normal

DEFF Research Database (Denmark)

Parkinson, C; Kassem, M; Heickendorff, Lene

2003-01-01

of bone and soft tissue turnover, as well as levels of PTH and vitamin D metabolites, in 16 patients (nine males; median age, 52 yr; range, 28-78 yr) with active acromegaly (serum IGF-I at least 30% above upper limit of an age-related reference range). Serum procollagen III amino-terminal propeptide...... (PIIINP) and type I procollagen amino-terminal propeptide, osteocalcin (OC), bone-related alkaline phosphatase, C-terminal cross-linked telopeptide of type I collagen (CTx), albumin-corrected calcium, intact PTH, 25-hydroxy vitamin D, 1,25-dihydroxy vitamin D [1,25-(OH)(2) vit D], urinary type 1 collagen...... (CTx and urinary type 1 collagen cross-linked N-telopeptide/creatinine ratio). 1,25-(OH)(2) vit D decreased and intact PTH increased significantly, but 25-hydroxy vitamin D was unaffected. A significant decline in calculated calcium clearance was observed. The decrease in serum IGF-I correlated...

a case report

African Journals Online (AJOL)

LIVINGSTON

redness of the eyes, itching and irritation of the ... Once symptoms have eased, daily 'eyelid hygiene' can usually keep the symptoms to a minimum. ... after instillation of two drops of 0.05% novesine. - Tab. Vit. C, 2 tabs tds for 2weeks was also.

Case series

African Journals Online (AJOL)

abp

hémodialyse chronique en raison de sa longévité, son taux faible de complication et de mortalité par rapport aux pontages artério-veineux et aux cathéters. Cependant, il arrive assez souvent que l'on assiste à des.

supp3.doc

Indian Academy of Sciences (India)

Sathiyanathan Felixa, Pratap Kollub, Bala P.C.Raghupathya,c,*, Soon Kwan Jeongd,* and Andrews Nirmala Grace a,d,*. a Centre for Nanotechnology Research, VIT University, Vellore - 632014, Tamil Nadu, India. b DST-INSPIRE Faculty, Department of Metallurgical Engineering and Materials Science, Indian Institute of ...

Synthesis, crystal structures, spectroscopic characterization and in ...

Indian Academy of Sciences (India)

cDepartment of Biotechnology, School of Bio-Sciences and Technology, VIT University, Vellore,. Tamil Nadu .... M. The lower molar conductivity values of 3 and 3.5. −1 cm2mol. −1 ..... the tolerance of pancreatic β-cells against oxida- tive stress ...

Hetkehitt : Rihanna feat. Jay-Z "Umbrella". Mikk Saar rootslaste lahjendatud versioonis. Kilehäälne Stelle trummari tehtud meigiga / Piret Järvis

Index Scriptorium Estoniae

Järvis, Piret, 1984-

2007-01-01

Laulust. Heliplaadist "See on see". Psühhedeelset power-poppi viljelevast ansamblist Stella, debüütplaadi "First Kiss" esitluskontserdist 18. mail Tallinnas üritusel Mutant Disco (bändi lugusid saab kuulata: www.myspace.com/stellatallinn)

Neurotoxic profiles of vanadium when administered at the onset of ...

African Journals Online (AJOL)

Pups exposed to vanadium showed reduced upper body strength which was protected by administration of vit E. Routine histology with Haematoxylin and Eosin revealed increased necrotic neurons of the medulla in vanadium exposed rats. Cresyl Violet stain showed depletion of the external granular layer of the ...

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African Journals Online (AJOL)

Dr Olaleye

morphometric evaluations were not significantly different, behavioural studies show that CLSE and VIT E significantly (p<0.05) increased the ..... Histogram of behavioural tests in the open field experiments in control and treated groups. (A) Horizontal .... ciprofloxacin on testicular function of male Guinea pigs,. Asian Jour.

Chemical cross-linking with thiol-cleavable reagents combined with differential mass spectrometric peptide mapping--a novel approach to assess intermolecular protein contacts

DEFF Research Database (Denmark)

Bennett, K L; Kussmann, M; Björk, P

2000-01-01

The intermolecular contact regions between monomers of the homodimeric DNA binding protein ParR and the interaction between the glycoproteins CD28 and CD80 were investigated using a strategy that combined chemical cross-linking with differential MALDI-MS analyses. ParR dimers were modified in vit...

supp11a.doc

Indian Academy of Sciences (India)

aDepartment of Chemical Engineering, Sunchon National University, 315 Maegok Dong, Suncheon 540-742, Chonnam, South Korea. bMaterials Chemistry Division, School of Advanced Sciences, VIT University, Vellore 632014, Tamilnadu, India. E-mail: ismoon@sunchon.ac.kr. Figure S1. FT-IR spectrum of compound 1.

Early postnatal calcium and phosphorus metabolism in preterm infants

NARCIS (Netherlands)

Christmann, V.; Grauw, A.M. de; Visser, R.; Matthijsse, R.P.; Goudoever, J.B. van; Heijst, A.F.J. van

2014-01-01

OBJECTIVES: Bone mineralisation in preterm infants is related to the supply of calcium (Ca) and phosphorus (P). We increased the amount of minerals in parenteral nutrition (PN) for preterm infants and evaluated postnatal Ca and P metabolism in relation to mineral and vitamin D (vitD) intake.

Venom immunotherapy for preventing allergic reactions to insect stings : a systematic review and health economic analysis

NARCIS (Netherlands)

Boyle, R.; Elremeli, M.; Cherry, M.; Oude Elberink, J.N.G.; Bulsara, M.; Mahon, J.; Daniels, M.; Hockenhull, J.

2012-01-01

Background Venom immunotherapy (VIT) is commonly used for preventing further allergic reactions to insect stings in people who have had a sting reaction. The efficacy and safety of this treatment has not previously been assessed by a high-quality systematic review. Objectives To assess the effects

Nephrogenic diabetes insipidus in a patient with L1 syndrome : A new report of a contiguous gene deletion syndrome including L1CAM and AVPR2

NARCIS (Netherlands)

Knops, Noel B. B.; Bos, Krista K.; Kerstjens, Mieke; van Dael, Karin; Vos, Yvonne J.

2008-01-01

We report on.in infant boy \\vitli congenital hydrocephatLis CILle to 1.1 syndrorne and p0lyUria dne to diabetes itisipidtis. We initially believed Ins excessive Lirine loss was froin central diabetes insipidLIS and diat the cerebral inalforniation caused a secondary insufficient pitnitary

assessment of the levels sment of the levels of heavy metals in three ...

African Journals Online (AJOL)

userpc

terrestrial ecosystem, which can provide vit ecosystem services, such as fisheries resource and habitant, food for migratory and residen animals, aesthetic landscape, and recreation opportunities for human populations (Wang an .... labelled, stored in a large plastic container with ice blocks and transported to the laboratory ...

Dgroup: DG00129 [KEGG MEDICUS

Lifescience Database Archive (English)

Full Text Available DG00129 Chemical ... DGroup Tocopherol (vit E) ... D02332 ... Tocopherol (JP17) D01406 ... Toco...pherol calcium succinate (JP17) D01530 ... Tocopherol nicotinate (JP17) ... D01735 ... Tocopherol acetate (JP17) ... D08612 ... Tocopherol succinate ... ATC code: A11HA03 Vitamin E ...

Witz, Lust und Aggression

DEFF Research Database (Denmark)

Rösing, Lilian Munk

2014-01-01

Artiklen beskæftiger sig med forholdet mellem vits, lyst og aggression med udgangspunkt i lysten ved aggressiv litterær humor, eksemplificeret ved tekststeder fra Shakespeares Hamlet. Der argumenteres for, at aggressionen eller angrebet er et fælles centralt aspekt ved Sigmund Freuds og Friedrich...

A Two-Tiered-Testing Decision Tree for Assays in the USEPA-EDSP Screening Battery: Using 15 years of experience to improve screening and testing for endocrine active chemicals

Science.gov (United States)

Outline of the presentationEDCs – from 1991 to 1996 – Wingspread and Our Stolen Future 1996 – FQPA and SDWA mandates endocrine screening 1996-1998 – EDSTAC (the assays, debates over modes of action included) The final battery – EAT in vivo and in vit...

Die Dokter-Pasient Rolverwantskap

African Journals Online (AJOL)

17 Aug 1974 ... en as sodanig 'n ral sped in die persoon se siektegedrag en uiteindelike patroon van hulpsoeke. SIEKEROL EN SOSIALISERING IN DIE. PASIENTROL ..... more cumulative, resulting in a more gradual social and professional maturation.' Vit die voorafgaande word dit duidelik dat Dewy se pragmatiese ...

Diagnostic value of lipids, total antioxidants, and trace metals in ...

African Journals Online (AJOL)

Materials and Methods: Anthropometric characteristics, total prostate specific antigen (tPSA), serum lipids (total cholesterol, LDL cholesterol, HDL cholesterol, and triglycerides), Vit. E, total antioxidant status (TAS), and trace metals (Se, Cu, Fe, Zn, and Mn) were determined in 40 patients with histopathological diagnosis of ...

Tõsielust midagi jaburamat ei ole / Kristjan Ots

Index Scriptorium Estoniae

Ots, Kristjan

2004-01-01

PÖFFi filme - mängufilmid "Kadudega tuleb arvestada" ("Mit Verlust ist zu Rechnen") : režissöör Ulrich Seidl : Austria 1992 ja "Tş̌ehhi unelm" ("Česky sen") : režissöörid Vit Klusak ja Filip Remunda : Tšehhi 2004

Severe immediate allergic reactions to grapes: part of a lipid transfer protein-associated clinical syndrome

NARCIS (Netherlands)

Vassilopoulou, Emilia; Zuidmeer, Laurian; Akkerdaas, Jaap; Tassios, Ioannis; Rigby, Neil R.; Mills, E. N. Clare; van Ree, Ronald; Saxoni-Papageorgiou, Photini; Papadopoulos, Nikolaos G.

2007-01-01

BACKGROUND: Grape allergy is considered rare; grape lipid transfer protein (LTP; Vit v 1), an endochitinase and a thaumatin-like protein (TLP) have been reported as grape allergens. A considerable number of patients have referred to our department for severe reactions to grapes, and several IgE

In ovo Technology and newcastle disease resistance in Japanese Quail

International Nuclear Information System (INIS)

Ezzat, I.E.; Abu-Taleb, A.M.; Ali, I.E.; Shabana, M.K.

2001-01-01

A total number of 1600 fertile eggs from japanese quail birds were used in this study. The eggs were divided into eight groups then incubated. NDV and vite were administered to the groups of eggs in Ovo injection at day 14 from incubation. Hatchability, body weight, egg production and mortality were recorded weekly for each group. Five blood samples were collected weekly from each group to measure total serum proteins, albumin, globulin, T3, T4 and HI titre. The results of this work revealed an increase in total serum proteins, globulin, and a decrease in t3, T4 and albumin values of the in ove vaccinating groups. Also HI titre recorded higher values due to ovo vaccination alone or combined with vit. E. It was noticed that the group injected by inactive vaccine plus vit. E registered high increases in hatchability, body weight and egg production beside a decrease in mortality

Bee venom enhances the differentiation of human regulatory T cells.

Science.gov (United States)

Caramalho, I; Melo, A; Pedro, E; Barbosa, M M P; Victorino, R M M; Pereira Santos, M C; Sousa, A E

2015-10-01

Venom-specific immunotherapy (VIT) is well recognized by its efficacy, and compelling evidence implicates regulatory T cells (Tregs) in the underlying tolerogenic mechanisms. Additionally, hymenoptera venom has for a long time been claimed to modulate immunity. Here, we investigated the putative role of bee venom (Bv) in human FOXP3-expressing Treg homeostasis and differentiation, irrespective of the donors' allergic status. We found that Bv significantly enhanced the differentiation of FOXP3-expressing cells both from conventional naïve CD4 T cells and mature CD4 thymocytes, a property that may contribute to the VIT's capacity to expand circulating Tregs in allergic individuals. We expect that our data enlightening the Treg-mediated immunomodulatory properties of Bv regardless of TCR specificity, to have application in other allergies, as well as in other clinical settings, such as autoimmunity and transplantation. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

AVALIAÇÃO DA IMAGEM TURÍSTICA DE SANTA VITÓRIA DO PALMAR (RS, BRASIL, NA PERSPECTIVA DOS RESIDENTES

Directory of Open Access Journals (Sweden)

Melise de Lima Pereira

2017-06-01

Full Text Available O presente artigo busca identificar os fatores que compõem a imagem turística do destino Santa Vitória do Palmar (RS, Brasil, na perspectiva dos residentes, como medida de manifestação da identidade. A pesquisa possui caráter exploratório, descritivo, empírico e com abordagem predominantemente quantitativa. Foi utilizada uma amostra, não probabilística por conveniência, composta por 93 respondentes, colhida diretamente no município, durante outubro de 2015. Através de Análise Fatorial Exploratória foram identificados os componentes da imagem turística de Santa Vitória do Palmar, que são: Lugares históricos e serviços ofertados, opções de entretenimento, atrativos turísticos, hotelaria e transmissão da cultura local, higiene e comércio e fama do município.

Growth and hematology of juvenile pacu Piaractus mesopotamicus (Holmberg 1887 fed with increasing levels of vitamin E (DL-α-tocopheryl acetate

Directory of Open Access Journals (Sweden)

RICARDO Y. SADO

2013-03-01

Full Text Available Intensive fish production systems are characterized by 100% artificial feeding, so any dietary imbalances or deficiencies may lead to diseases outbreaks and economic losses. This study was set out to determine the effects of increasing levels of dietary vitamin E on growth and hematology of juvenile pacu. Fishes were fed for 90 days, twice a day until apparent satiation with semi-purified diets containing 0.0; 25; 50; 150; 300 or 600 mg.kg−1 diet DL-α-tocopheryl acetate in a completely randomized design trial (n=4; biometrical and hematological data were collected and analyzed. Fishes fed with vit E diet (150 mg.kg−1 showed higher (p<0.05 weight gain and specific growth. Hematocrit, erythroblast number and total plasma protein were increased (p<0.05 in fishes fed diet with no vit E diet. Vitamin E supplementation in artificial diets for pacu is essential for growth and maintenance of normal erythropoiesis.

Serum magnesium and not vitamin D is associated with better QoL in COPD

DEFF Research Database (Denmark)

Hashim Ali Hussein, Sarah; Nielsen, Lars Peter; Dolberg, Mette K.Bøgebjerg

2015-01-01

BACKGROUND: Deficiency of serum levels of vitamin D3 (se vitD), magnesium (se Mg) and calcium (se Ca) may be associated with increased exacerbation risk in chronic obstructive pulmonary disease (COPD). However, associations with other aspects of COPD, e.g. lung function and quality of life (QoL),...

Comparison of Different Test Construction Strategies in the Development of a Gender Fair Interest Inventory Using Verbs

Science.gov (United States)

Wetzel, Eunike; Hell, Benedikt; Passler, Katja

2012-01-01

Three test construction strategies are described and illustrated in the development of the Verb Interest Test (VIT), an inventory that assesses vocational interests using verbs. Verbs might be a promising alternative to the descriptions of occupational activities used in most vocational interest inventories because they are context-independent,…

Java project on periodontal diseases: effect of vitamin C/calcium threonate/citrus flavonoids supplementation on periodontal pathogens, CRP and HbA1c

NARCIS (Netherlands)

Amaliya, A.; Laine, M.L.; Loos, B.G.; van der Velden, U.

2015-01-01

Objective To assess in a periodontally diseased rural population deprived from regular dental care and having poor dietary conditions, the effect of vitamin C/calcium threonate/citrus flavonoids (VitC/Ca/Fl) supplementation on subgingival microbiota and plasma levels of vitamin C, HbA1c and hsCRP.

Early postnatal calcium and phosphorus metabolism in preterm infants

NARCIS (Netherlands)

Christmann, Viola; de Grauw, Anne M.; Visser, Reina; Matthijsse, René P.; van Goudoever, Johannes B.; van Heijst, Arno F. J.

2014-01-01

Bone mineralisation in preterm infants is related to the supply of calcium (Ca) and phosphorus (P). We increased the amount of minerals in parenteral nutrition (PN) for preterm infants and evaluated postnatal Ca and P metabolism in relation to mineral and vitamin D (vitD) intake. Preterm infants,

Neuroprotective potential of Citrullus lanatus seed extract and ...

African Journals Online (AJOL)

Mercury chloride toxicity continues to be relevant in the advent of increased interest in mining activity in Nigeria. The neuroprotective potential of Citrullus lanatus seed extract (CLSE) (Watermelon seed) and vitamin E (VIT E) on mercury chloride intoxication on the frontal cerebral cortex of male rats was investigated.

Soutien analytique pour les pays d'Afrique et des Caraïbes en ...

International Development Research Centre (IDRC) Digital Library (Canada)

Dans le dernier numéro du bulletin du BRAS, voyez comment l'Afrique a évité ... financé par le Centre de recherches pour le développement international (CRDI). ... Les politiques de taxation appliquées dans les pays en développement et, ...

Gênero, etnia e movimentos sociais na história da educação

OpenAIRE

Forde, Gustavo Henrique Araújo; Instituto Federal do Espírito Santo

2013-01-01

FRANCO, Sebastião Pimentel; SÁ, Nicanor Palhares (Org.). Gênero, etnia e movimentos sociais nahistória da educação. Vitória – ES: EDUFES, 2011.(Coleção Horizontes da pesquisa em História daEducação no Brasil, 9).

Plasma vitamins and essential trace elements in newly diagnosed pulmonary tuberculosis patients and at different durations of anti-tuberculosis chemotherapy

Directory of Open Access Journals (Sweden)

V.F. Edem

2015-07-01

This study concluded that there is micronutrient (Fe, Zn, Cu, Vit A, C, D and E malnutrition in tuberculosis patients at diagnosis and throughout the duration (6 months of chemotherapy. Supplementation with vitamins and zinc is advised within the first 4 months of commencing anti-tuberculosis chemotherapy.

Advanced productivity forecast using petrophysical wireline data calibrated with MDT tests and numerical reservoir simulation

Energy Technology Data Exchange (ETDEWEB)

Andre, Carlos de [PETROBRAS, Rio de Janeiro, RJ (Brazil); Canas, Jesus A.; Low, Steven; Barreto, Wesley [Schlumberger, Houston, TX (United States)

2004-07-01

This paper describes an integrated and rigorous approach for viscous and middle oil reservoir productivity evaluation using petrophysical models calibrated with permeability derived from mini tests (Dual Packer) and Vertical Interference Tests (VIT) from open hole wire line testers (MDT SLB TM). It describes the process from Dual Packer Test and VIT pre-job design, evaluation via analytical and inverse simulation modeling, calibration and up scaling of petrophysical data into a numerical model, history matching of Dual Packer Tests and VIT with numerical simulation modeling. Finally, after developing a dynamic calibrated model, we perform productivity forecasts of different well configurations (vertical, horizontal and multilateral wells) for several deep offshore oil reservoirs in order to support well testing activities and future development strategies. The objective was to characterize formation static and dynamic properties early in the field development process to optimize well testing design, extended well test (EWT) and support the development strategies in deep offshore viscous oil reservoirs. This type of oil has limitations to flow naturally to surface and special lifting equipment is required for smooth optimum well testing/production. The integrated analysis gave a good overall picture of the formation, including permeability anisotropy and fluid dynamics. Subsequent analysis of different well configurations and lifting schemes allows maximizing formation productivity. The simulation and calibration results are compared to measured well test data. Results from this work shows that if the various petrophysical and fluid properties sources are integrated properly an accurate well productivity model can be achieved. If done early in the field development program, this time/knowledge gain could reduce the risk and maximize the development profitability of new blocks (value of the information). (author)

Tentativas de estabilização e crescimento por via da dívida externa: a economia brasileira de quatro décadas

Directory of Open Access Journals (Sweden)

Barbara Fritz

2014-06-01

Full Text Available Dada a reestruturação do setor produtivo, que vem ocorrendo desde os anos noventa, a maximização do superávit comercial depende não somente de grandes esforços em aumentar as exportações, mas também, a curto prazo, da repressão das importações. Desta maneira, a estagnação da economia, produzida por uma política monetária muito restritiva, tornou-se necessária para a sobrevivência a esta crise da dívida. As perspectivas futuras, a partir desta ótica que considera o endividamento o estrangulamento central da economia, não podem ser otimistas. O que se pode esperar no máximo é uma continuação do processo de Stop-and-Go. Se a economia brasileira voltar a crescer – o que é muito provável para o ano de 2004 – o superávit externo vai se reduzir; e se esta redução não for acompanhada por um aumento das importações de capital, o crescimento terá que ser imediatamente freado outra vez por uma política monetária restritiva, quer dizer uma outra fase de Stop. E esta impossibilitará novamente que os investimentos internos sejam reativados, condição necessária para poder alcançar um crescimento sustentado, apoiado em um superávit comercial baseado, por sua vez, em competitividade externa, e não na repressão do mercado interno.

The Effects of Mangiferin (Mangifera indica L) in Doxorubicin-induced Cardiotoxicity in Rats.

Science.gov (United States)

Arozal, W; Suyatna, F D; Juniantito, V; Rosdiana, D S; Amurugam, S; Aulia, R; Monayo, E R; Siswandi, R

2015-11-01

The cardiotoxicity effect of doxorubicin (DOX), a widely used antitumor agent has restricted its clinical application. The aim of the current study was to explore the potential protective effect of mangiferin, a naturally occurring glucosylxanthone, that have antioxidant activity by its iron-complexing ability in mitochondria, against DOX-induced cardiac toxicity in rats in comparison with other antioxidants namely Sylimarin (SYL) and Vitamin E (VitE). Mangiferin was given orally to rats at a dose of 50, and 100 mg/kg for 5 weeks, and DOX was injected at a total dose of 15 mg/kg. Cardiac toxicity was evaluated by lactate dehydrogenase and creatine kinase in the serum, malondialdehyde (MDA) level in plasma and cardiac tissue, and antioxidant enzyme superoxide dismutase (SOD) in cardiac tissue. Mangiferin protected against DOX-induced increased mortality and electrocardiogram abnormality and decreased biochemical markers of cardiac toxicity i. e., lactate dehydrogenase and creatine phosphokinase isoenzyme. In addition, elevation of plasma and cardiac tissue levels of MDA in response to DOX treatment were significantly attenuated. The reduction of cardiac activity of SOD was significantly reduced in contrast with the other antioxidant SYL and Vit E. Histopathologically, mangiferin treatment showed significant reduction in inflammatory cell number, fibrotic area, and necrotic foci as compared with DOX only-treated rats. These results suggested that mangiferin had better protective effect against DOX-induced cardiac toxicity in comparison with SYL and VitE, thus besides the antioxidant activity, different mechanism may be involved in the action of mangiferin and need to be clarified in the future studies. © Georg Thieme Verlag KG Stuttgart · New York.

The development of composition and technology for the capsulated drug based on bee pollen and honey powder. Announcement 3.

Directory of Open Access Journals (Sweden)

B. Т. Kudrik

2016-12-01

Full Text Available Aim. To develop experimentally and to substantiate theoretically both in pharmaceutical and industrial conditions the technology of manufacturing for capsules with the immunomodulatory action (provisional name «Api-Immuno-Vit» basing on pharmacotechnological and physical-chemical studies. Also to study the effect of excipients, the residual moisture and the particle size of capsules on technological parameters of mixtures quality, as well as to substantiate the choice of the size for solid gelatin capsules. Material and methods. The study objects were the experimental samples of the mixtures of «Api-Immuno-Vit» capsules with such active pharmaceutical ingredients (API as bee pollen (DSTU 7074:2009, GOST 31776-2012 and honey powder (TU U 10.8-39834691-001:2015, as well as aerosil and mannitol, which were used as excipients. Results. The pharmacotechnological tests of the experimental samples of the mixtures (particle size distribution, moisture content, fluidity, bulk density and tapped density, angle of repose, uniformity of mixing were carried out using the conventional methods given in the State Pharmacopoeia of Ukraine. The influence of particle size distribution of the granulate on technological parameters of the mixture quality in capsules has been studied. It has been found that the increase of residual moisture leads to decrease of fluidity, and therefore, the residual moisture of the mixture for encapsulation should be less than 1.5%. It has been also noted that during the experiment the presence of large and medium fractions is important for the particle size distribution since fluidity and the bulk density of the mixtures significantly reduce if the fine fraction in more than 30% in amount. Сonclusions. The results of the experimental studies were used in developing the flowchart of «Api-Immuno-Vit» capsules manufacturing. Taking into account the physical-chemical properties and the pharmacotechnological tests the flowchart of

Evolution of Serum 25OHD in Response to Vitamin D3-Fortified Yogurts Consumed by Healthy Menopausal Women: A 6-Month Randomized Controlled Trial Assessing the Interactions between Doses, Baseline Vitamin D Status, and Seasonality.

Science.gov (United States)

Bonjour, Jean-Philippe; Dontot-Payen, Flore; Rouy, Emilien; Walrand, Stephane; Rousseau, Brigitte

2018-01-01

Adequate vitamin D status contributes to bone fragility risk reduction and possibly other pathological conditions that occur with aging. In response to pharmaceutical vitamin D 3 supplements, several studies have documented the influence of doses, baseline status, and seasonality on serum 25-hydroyvitamin D (s25OHD). Using fortified yogurt, we investigated in one randomized controlled trial how both baseline status, as assessed by measuring s25OHD prior the onset of the trial, and the season of enrollment quantitatively influenced the response to the supplemented (Suppl.) of vitamin D 3 (VitD 3 ) in healthy community-dwelling women. A 24-week controlled trial was conducted in menopausal women (mean age: 61.5). Participants were randomized into 3 groups (Gr): Gr.Suppl.0, time controls maintaining dietary habits; Gr.Suppl.5 and Gr.Suppl.10 consuming one and two 125-g servings of VitD 3 -fortified yogurts with 5- and 10-µg daily doses, respectively. The 16 intervention weeks lasted from early January to mid-August, the 8 follow-up weeks, without product, from late August to mid-October. Before enrollment, subjects were randomized into 2 s25OHD strata: low stratum (LoStr): 25-50 nmol/L; high stratum (HiStr): >50-75 nmol/L. All enrolled participants adhered to the protocol throughout the 24-week study: Gr.Suppl.0 (n = 45), Gr.Suppl.5 (n = 44), and Gr.Suppl.10 (n = 44). Over the 16 intervention and 8 follow-up weeks, s25OHD increased in both supplemented groups, more in Gr.Suppl.10 than in Gr.Suppl.5. At the end of the intervention, the subject proportion with s25OHD ≥ 50 nmol/L was 37.8, 54.5, and 63.6% in Gr.Suppl.0, Gr.Suppl.5, and Gr.Suppl.10, respectively. The constant rate of s25OHD per supplemental VitD 3 microgram was greater in LoStr than HiStr. The s25OHD increase was greater with late (mid-March) than early (mid-January) inclusion. This randomized trial demonstrates (1) a dose-dependent s25OHD improvement related to fortified yogurt consumption; (2) an

Evolution of Serum 25OHD in Response to Vitamin D3–Fortified Yogurts Consumed by Healthy Menopausal Women: A 6-Month Randomized Controlled Trial Assessing the Interactions between Doses, Baseline Vitamin D Status, and Seasonality

Science.gov (United States)

Bonjour, Jean-Philippe; Dontot-Payen, Flore; Rouy, Emilien; Walrand, Stephane; Rousseau, Brigitte

2018-01-01

ABSTRACT Background: Adequate vitamin D status contributes to bone fragility risk reduction and possibly other pathological conditions that occur with aging. In response to pharmaceutical vitamin D3 supplements, several studies have documented the influence of doses, baseline status, and seasonality on serum 25-hydroyvitamin D (s25OHD). Objective: Using fortified yogurt, we investigated in one randomized controlled trial how both baseline status, as assessed by measuring s25OHD prior the onset of the trial, and the season of enrollment quantitatively influenced the response to the supplemented (Suppl.) of vitamin D3 (VitD3) in healthy community-dwelling women. Methods: A 24-week controlled trial was conducted in menopausal women (mean age: 61.5). Participants were randomized into 3 groups (Gr): Gr.Suppl.0, time controls maintaining dietary habits; Gr.Suppl.5 and Gr.Suppl.10 consuming one and two 125-g servings of VitD3-fortified yogurts with 5- and 10-µg daily doses, respectively. The 16 intervention weeks lasted from early January to mid-August, the 8 follow-up weeks, without product, from late August to mid-October. Before enrollment, subjects were randomized into 2 s25OHD strata: low stratum (LoStr): 25–50 nmol/L; high stratum (HiStr): >50–75 nmol/L. Results: All enrolled participants adhered to the protocol throughout the 24-week study: Gr.Suppl.0 (n = 45), Gr.Suppl.5 (n = 44), and Gr.Suppl.10 (n = 44). Over the 16 intervention and 8 follow-up weeks, s25OHD increased in both supplemented groups, more in Gr.Suppl.10 than in Gr.Suppl.5. At the end of the intervention, the subject proportion with s25OHD ≥ 50 nmol/L was 37.8, 54.5, and 63.6% in Gr.Suppl.0, Gr.Suppl.5, and Gr.Suppl.10, respectively. The constant rate of s25OHD per supplemental VitD3 microgram was greater in LoStr than HiStr. The s25OHD increase was greater with late (mid-March) than early (mid-January) inclusion. Conclusion: This randomized trial demonstrates (1) a dose-dependent s25OHD

Prevalência da síndrome de Sjögren primária em importante área metropolitana no Brasil Primary Sjögren's syndrome prevalence in a major metropolitan area in Brazil

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Valéria Valim

2013-02-01

Full Text Available Não há estudo anterior sobre a prevalência de síndrome de Sjögren (SS no Brasil. O objetivo deste estudo foi avaliar a prevalência de SS na população geral de Vitória, estado do Espírito Santo, Brasil. Trata-se de estudo epidemiológico, observacional e transversal com 1.205 indivíduos randomizados, cujas idades variaram de 18 a 65 anos, residentes em Vitória. Os indivíduos foram triados para xerostomia e xeroftalmia por meio de entrevista domiciliar. Aqueles com sintomas "sicca" foram convidados a comparecer ao hospital para realizar avaliação médica adicional, avaliação do fluxo salivar não estimulado, teste de Schirmer I, exame de sangue e biopsia de glândula salivar labial menor. Os sintomas "sicca" foram identificados em 18% (217 indivíduos da amostra. Dos 217 indivíduos com sintomas "sicca", 127 (58% estavam disponíveis para exame. Nessa amostra, 61,7% eram mulheres e 46,8% usavam medicação. A síndrome "sicca" foi confirmada em 12% deles por pelo menos um exame (fluxo salivar ou teste de Schirmer I. Dois pacientes (0,17% preencheram quatro critérios classificatórios americano-europeus (95% IC = 0,020-0,5983.There has been no previous prevalence study about of Sjögren's syndrome (SS in Brazil. The aim was to evaluate the SS prevalence in a general population in Vitória, ES, Brazil. This was an epidemiological, observational, and cross-sectional study conducted on 1,205 randomized people, aged 18-65 years, who lived in Vitória. The subjects were screened for xerostomia and xerofphthalmia through home interviews. Those with sicca symptoms were asked to report to a hospital for further medical evaluation, unstimulated salivary flow, Schirmer I test, blood analysis and minor labial salivary biopsy. Sicca symptoms were found in 18% (217 subjects of the sample. Of the 217 subjects with sicca symptoms, 127 (58% were available for examination. In this sample, 61.7% were female and 46.8% were under medication. Sicca

Les Comités mixtes de sécurité humaine (CMSH) au Burundi : un ...

African Journals Online (AJOL)

the political regime in place to find an explanatory framework for its abuses. As part of ... Pour rappel, le Burundi vit, depuis son indépendance en juillet 1962, des ... La mise en place des comités mixtes de sécurité en tant que nouveau régime.

Effects of dietary antioxidant supplementation in cow’s feed, milk processing and storage on lutein content and sensory quality

Science.gov (United States)

In this work, we studied the lutein content in milk as affected by lutein supplementation in the absence and presence of common antioxidants, vitamin E (Vit E), tea polyphenols (TP) and ethoxyquin (EQ) in cow’s feed, and by subsequent pasteurization (HTST and UHT) and storage. Results showed that l...

Diaspora Impact on the Capacity for Recovery from Conflict and Crisis

International Development Research Centre (IDRC) Digital Library (Canada)

Dans le dernier numéro du bulletin du BRAS, voyez comment l'Afrique a évité ... Le soutien apporté par le CRDI à BetterEvaluation, un réseau international visant à ... Les politiques de taxation appliquées dans les pays en développement et, ...

Bangalore, ville des nouvelles technologies

Directory of Open Access Journals (Sweden)

Clarisse Didelon

2003-06-01

Full Text Available Bangalore est devenue la Silicon Valley de l’Inde. Une partie de ses habitants y vit à l’occidentale mais le reste de la population souffre de la croissance spectaculaire de la ville. Face à l’insuffisance des infrastructures, Bangalore devient de moins en moins attractive pour les entreprises internationales.

DEVTA vitamin A schedule, 05/1999 - 04/2004

Indian Academy of Sciences (India)

First page Back Continue Last page Overview Graphics. Dosage: 200,000 IU vitamin A on the. Dosage: 200,000 IU vitamin A on the. 6-monthly mass treatment days. to all then aged 6-72 months. Vit A compliance: miss ~1.6 of 11 doses. Controls: get mean of ~1 non-trial dose.

Altered vitamin D status in liver tissue and blood plasma from Greenland sledge dogs (Canis familiaris) dietary exposed to organohalogen contaminated minke whale (Balaenoptera acuterostrata) blubber

DEFF Research Database (Denmark)

Sonne, Christian; Kirkegaard, Maja; Jakobsen, Jette

2014-01-01

This study compared vitamin D3 (vitD3) and 25-OH vitamin D3 (25OHD3) status in Greenland sledge dogs (Canis familiaris) given either minke whale (Balaenoptera acuterostrata) blubber high in organohalogen contaminants (OHCs) or clean porcine (Suis scrofa) fat for up to 636 days. A group of six...

Anne, ma soeur Anne, ne vois-tu rien venir? Que oui! | CRDI ...

International Development Research Centre (IDRC) Digital Library (Canada)

23 déc. 2010 ... Après tout, 60 pour cent de la population mondiale ne vit-elle pas dans un rayon ... explosifs... tous ces défis ne pourraient être relevés sans l'étude des montagnes. ... En outre, les dangers d'avalanches, d'éboulements et de ...

Model Pemberdayaan Pekarangan Di Kecamatan Rumbai Pesisir Kota Pekanbaru

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NIKEN NURWATI

2016-03-01

Full Text Available Tujuan penelitian ini adalah untuk menganalisis (1 Mengetahui potensi lahan  pekarangan dalam rangka program RPL (2 Menganalisis sumbangan pendapatan dan nilai gizi pangan dari pekarangan (3 Mengetahui respon masyarakat  terhadap program rumah pangan lestari (RPL (4.Mengetahui model pemberdayaan Pekarangan di Kecamatan Rumbai Pesisir Kota Pekanbaru Penelitian dilaksanakan dengan metode survey. Unit analisa dalam penelitian ini adalah rumahtangga.Data yang dikumpul dalam penelitian ini terdiri dari data primer dan data sekunder. Penarikan sampel dengan stratified random sampling berdasarkan rata-rata luas bangunan dan pekarangan pada kelurahan terpilih. Analisis data identitas sampel, pemanfaatan pekarangan dilakukan secara diskriptif, sedangkan analisis pendapatan dari pekarangan dilakukan dengan menggunakan rumus-rumus.TR = Y.Py. Pendapatan bersih usahatani pekarangan menggunakan dengan rumus : ∏ = TR-TC. Sedangkan kandungan zat gizi bahan makanan dihitung berdasarkan rumus sbb: KGij= (Bj/100 X GijX (BDDij/100.Hasil penelitian: (1 Kecamatan Rumbai Pesisir berpotensi untuk dikembangakan/ ditingkatkan pemberdayaan pekarangan pada semua strata luas lahan (100 m2 sampai dengan > 400m2. (2 Pola pemanfaatan pekarangan yang dimungkinkan adalah dengan teknik vertikultur/pot pada lahan sempit, penggunaan para-para atau vertikultur pada lahan yang tergenang air, usahatani di lahan pada lahan luas dan tidak terkena banjir. (3. Sumbangan pendapatan dari pekarangan sebagian besar (72,73% kurang dari Rp. 1.000.000,- /tahun. Hal ini menunjukkan rendahnya pemanfaatan pekarangan. (4Sumbangan gizi dari produksi pekarangan,  rata-rata 1.034.641 kal  Kalori, 53.722 gram protein, 17.763 gram lemak, 206.313 gram karbohidrat, 2.135.461 miligram kalium, 1.811.929 miligram phosphor, 24.067 miligram zat besi, 74.545.653 SI Vit A,  3.685 miligram Vit B1 dan  663.915 miligram Vit.C. (5. Respon masyarakat terhadap RPL masih rendah. hal ini karena adanya faktor

Serum alkaline phosphatase screening for vitamin D deficiency states

International Nuclear Information System (INIS)

Shaheen, S.; Barrakzai, Q.

2012-01-01

Objective: To determine whether serum vitamin D levels are correlated with serum levels of alkaline phosphatase or not. Study Design: Cross-sectional, observational study. Place and Duration of Study: Multi-centre study, conducted at Liaquat National Hospital and Medical College, National Medical Centre and Medicare Hospital, Karachi, from January to October 2009. Methodology: Patients attending the Orthopaedic OPDs with complaints of pain in different body regions and serum vitamin D/sub 3/ levels of greater or equal to 30 ng/ml were included in the study. Patients with vitamin D deficiency were further categorized into mild deficiency or insufficiency (vit. D/sub 3/ = 20-29 ng/ml), moderate deficiency (vit. D/sub 3/ = 5 - 19 ng/ml) and severe deficiency forms (vit. D/sub 3/ < 5 ng/ml). Pearson correlation was applied to test the correlation of serum alkaline phosphatase levels with serum vitamin D/sub 3/ levels. P-value < 0.05 was considered to be significant. Results: Out of 110 samples, 26 had mild (23%), 61 had moderate (55%) and 21 had severe (19.1%) vitamin D deficiencies. All of the patients in the three groups had alkaline phosphatase with in normal limits and the total mean value of the enzyme was 135.97 +- 68.14I U/L. The inter group comparison showed highest values of alkaline phosphatase in the moderate vitamin D deficiency group. The correlation coefficient of alkaline phosphatase and serum vitamin D/sub 3/ levels was r =0.05 (p =0.593). Conclusion: Serum vitamin D/sub 3/ levels may not be correlated with increased serum alkaline phosphatase levels. Therefore, alkaline phosphatase may not be used as a screening test to rule out vitamin D deficiency. (author)

INDICADORES DE INPUT DO MUNICÍPIO DE VITÓRIA DA CONQUISTA, BAHIA

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Celeste Dias Amorim

2014-09-01

Full Text Available Há trinta anos foi implantada a primeira instituição de ensino superior (IES na cidade de Vitória da Conquista, chamada Universidade Estadual do Sudoeste da Bahia (UESB. Contudo, nessa última década observa-se a sua ascensão e um intenso processo de criação de diversas outras instituições, tanto privadas como também públicas, aumentando grandemente a oferta de vagas para educação superior no referido município. O presente texto pretende analisar os indicadores de input que contribuam para o desenvolvimento do município de Vitória da Conquista, primordialmente nos aspectos sociais, econômicos, educacionais, ambientais e de saúde, enquanto município polarizador educacional da região Sudoeste da Bahia. Foram tomadas como referencial basilar as informações fornecidas por órgãos locais, como: agência do IBGE, relatórios das instituições educacionais privadas e públicas de ensino superior, bem como obras de autores regionais. Para tanto, foi necessário um recorte temporal analítico, tomando como base a primeira década de século XXI. O estudo do desenvolvimento de Vitória da Conquista em diversos segmentos demonstra que os dados apontados pelos indicadores de input (recursos humanos IDH, PIB, IES utilizados estão intimamente relacionados ao sistema de educação desenvolvido na região, além de fornecerem importantes pontos de referência no planejamento de investimentos para o presente e para o futuro, bem como da importância da transferência da ciência para tecnologia de forma a não perder de vista como este afeta e é afetado pelo estilo de desenvolvimento proposto.

Effect of vitamin B1 and mixtures of B1 with other vitamins on cytostatica efficiency under irradiation. A study in vitro

International Nuclear Information System (INIS)

Heinrich, E.; Getoff, N.

2002-01-01

Complete text of publication follows. In the last decades the research of malignant tumors (oncology) became a complex interdisciplinary branch of the modern sciences. In addition to other disciplines the radiation chemistry and radiation biology, applied in this respect, led to new and deeper understanding and knowledge in the radiation therapy of cancer. Following this line and by a combined use of both methods, studies in vitro for testing cytostatica activity and pulse radiolysis for characterisation of the corresponding transients, it was possible to gain a deeper and extensive understanding of complex reaction mechanisms. In addition to valuable biological data we have established that the cytostatica efficiency, e.g. of mitomycin C (MMC) can be very strongly increased in the presence of the vitamins C, E and β-carotene. As an essential step in this respect a cascade electron transfer with the sequence: vit. C → vit. E· + /vit. E → β-car. · + /β-car. → MMC/MMC· - was established. In the present work the ability of vitamin B1 (thiamine) to act under given conditions as cytostaticum or/and as radiation protecting agent will be reported. Extensive investigations in vitro (E. coli bacteria AB 1157 were used as a model) in different media showed that with a combination of vitamin B1 with sanazole (AK-2123) a synergistic effect occurs, which results in a very strong cytostatica enhancement. The presence of vitamin C in the system strongly contributes in this respect. The influence on the sanazole efficiency by other vitamins (E and β-carotene) added individually or in mixture will also be reported. The obtained results are of importance for the radiation therapy of cancer patients. The paper also demonstrates the significant role of radiation chemistry in radiation biology and medicine

Vacuolar iron transporter BnMEB2 is involved in enhancing iron tolerance of Brassica napus

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Wei Zhu

2016-09-01

Full Text Available Iron toxicity is a major nutrient disorder that severely affects crop development and yield. Vacuolar detoxification of metal stress is an important strategy for plants to survive and adapt to this adverse environment. Vacuolar iron transporter (VIT members are involved in this process and play essential roles in iron storage and transport. In this study, a rapeseed VIT gene BnMEB2 (BnaC07g30170D was identified. BnMEB2 is a homolog to Arabidopsis MEB2 (At5g24290 and acts as a detoxifier in vacuolar sequestration of divalent metal. Transient expression analysis revealed that BnMEB2 was localized to the vacuolar membrane. Q-PCR detection showed a high expression of BnMEB2 in mature (60-day-old leaves and could be obviously induced by exogenous iron stress in both roots and leaves. Over-expressed BnMEB2 in both Arabidopsis wild type and meb2 mutant seedlings resulted in greatly improved iron tolerability with no significant changes in the expression level of other vacuolar iron transporter genes. The mutant meb2 grew slowly and its root hair elongation was inhibited under high iron concentration condition while BnMEB2 over-expressed transgenic plants of the mutant restored the phenotypes with apparently higher iron storage in roots and dramatically increased iron content in the whole plant. Taken together, these results suggested that BnMEB2 was a VIT gene in rapeseed which was necessary for safe storage and vacuole detoxification function of excess iron to enhance the tolerance of iron toxicity. This research sheds light on a potentially new strategy for attenuating hazardous metal stress from environment and improving iron biofortification in Brassicaceae crops.

Impact of Hymenoptera venom allergy and the effects of specific venom immunotherapy on mast cell metabolites in sensitized children

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Ewa Cichocka-Jarosz

2014-06-01

Full Text Available introduction and objective. Mast cells (MC are effector cells during severe systemic reactions (SR to Hymenoptera stings. Venom specific immunotherapy (VIT is the treatment of choice for prevention of SR to stings. Tryptase and prostaglandin D[sub]2[/sub] metabolites (PGD[sub]2[/sub] are the markers of MC activation. The study design was to 1. compare baseline values of serum tryptase concentration (BST and PGD[sub]2[/sub] metabolites in children with/without venom sensitization, 2. to evaluate an influence of rush VIT on MC markers in treated children. materials and methods. Sensitized group: 25 children with SR to Hymenoptera sting. Control group: 19 healthy children. Active treatment: 5-day-rush-VIT. BST was evaluated by ImmunoCAP, PGD[sub]2[/sub] metabolites in blood and urine by GC-NICI-MS. results. The baseline blood levels of MC markers were significantly higher, while urinary concentration of 9α,11β-PGF2 was significantly lower in the whole group of venom-sensitized children compared to controls. Severity of SR showed negative correlation with urinary PGD[sub]2[/sub] metabolites, while positive with plasma 9α,11β-PGF2 and BST concentration The highest sensitivity was obtained for plasma 9α,11β-PGF2 whereas the highest specificity for urinary PGD-M. conclusions. In children with IgE-mediated SR to Hymenoptera stings, elevation of baseline values of PGD2 metabolites in blood is accompanied by decreased excretion of its urinary metabolites. Assessment of stable PGD[sub]2 [/sub] metabolites might serve as an independent MC marker to identify allergic children. There is an association between urinary PGD[sub]2[/sub] metabolites and severity of the SR to Hymenoptera stings.

Penconazole alters redox status, cholinergic function and lung's histoarchitecture of adult rats: Reversal effect of vitamin E.

Science.gov (United States)

Chaâbane, Mariem; Elwej, Awatef; Ghorbel, Imen; Chelly, Sabrine; Mnif, Hela; Boudawara, Tahia; Ellouze Chaabouni, Semia; Zeghal, Najiba; Soudani, Nejla

2018-06-01

The present study pertains to the possible adverse effects of penconazole exposure on the lung of adult rats, and to the potential ability of vitamin E (Vit E) in mitigating the toxicity induced by this fungicide. Male Wistar rats were divided into four groups of six animals each: Group I (Controls): rats drank distilled water; Group II (PEN): rats received, by gavage, 50 mg/kg body weight (1/40 LD 50 ) of penconazole every 2 days during 10 days; Group III (Vit E): rats received daily 100 mg α-tocopherol acetate/kg body weight during 10 days by gavage; and Group IV (Vit E + PEN): rats received both vitamin E (100 mg α-tocopherol acetate/kg body weight) and penconazole (50 mg/kg body weight), being vitamin E given as a daily dosage and penconazole every 2 days, by gavage during 10 days. Results showed that penconazole induced oxidative stress in the lung demonstrated by an increase in malondialdehyde (+77%), hydrogen peroxide (+58%) and advanced oxidation protein product (+22%) levels, as compared to the controls. Furthermore, a decrease in the activities of catalase (-41%), superoxide dismutase (-45%), glutathione peroxidase (-23%) and acetylcholinesterase (-67%), and an increase in the levels of non-protein thiols (+17%), glutathione (+7%) and vitamin C (+44%) were registered. Abnormalities in lung histological sections such as alveolar edema, infiltration of inflammatory cells (leukocytes) and emphysema, were also observed following penconazole exposure. Vitamin E ameliorated the biochemical parameters, as well as the histological impairments induced by this fungicide. In conclusion, our study demonstrated that vitamin E, a natural antioxidant, was effective in alleviating penconazole-induced lung damage in Wistar rats. Copyright © 2018 Elsevier Masson SAS. All rights reserved.

Influence of Moringa oleifera extract, vitamin C, and sodium bicarbonate on heat stress-induced HSP70 expression and cellular immune response in rabbits.

Science.gov (United States)

Abdel-Latif, Mahmoud; Sakran, Thabet; Badawi, Yasser K; Abdel-Hady, Doaa S

2018-05-04

The current study aimed to test the effect of Moringa oleifera extract (MOE), vitamin (Vit) C, and sodium bicarbonate (NaHCO 3 ) on heat stress (HS)-induced alterations in rabbits. Five groups of rabbits were designed as control, HS, HS + MOE, HS + Vit C, and HS + NaHCO 3 . HS groups were exposed to high temperatures, while treatments were given in drinking water for 6 weeks. Levels of blood cortisol, leptin, IFN-γ, TNF-α, and IL-10 were assayed using ELISA, while adrenaline was assayed calorimetrically. Expression of HSP70, FOXP3, T cell receptor (TCR) γ, and δ mRNA was tested using real-time (RT)-PCR, while HSP70 protein expression was tested using western blotting in liver and kidney tissues. Infiltration of regulatory T cells (Treg; CD25 + ) and NK (CD56 + ) cells were tested using immunohistochemistry (IHC). The levels of liver enzymes (ALT & AST), urea, and creatinine were assayed calorimetrically, while body weight gain (BWG) and feed conversion ratio (FCR) were calculated. The results showed increased levels of cortisol, adrenaline, leptin, IFN-γ, TNF-α, ALT, AST, urea, and creatinine but decreased IL-10 in the HS group. Increased expression of HSP70 on both mRNA and protein levels was associated with increased NK and γδ T cells versus decreased Treg cell infiltration in liver and kidney tissues of the HS group. In the same group, BWG was decreased, while FCR was increased with respect to the control group. All treatments used in this study reversed the effects of HS significantly. In conclusion, MOE, Vit C, and NaHCO 3 can be added to rabbit diets for the amelioration of HS-induced symptoms.

The effect of synthetic antioxidants on the oxidative stability of biodiesel

CSIR Research Space (South Africa)

Focke, WW

2012-04-01

Full Text Available ral acti vit ies. T h is is widel y pr esented as its main advantage [2] . In addit ion, b iodi esel fuel is biodegr ad a b l e [3] [ 6] contains insignificant amounts of sulphur [1] and is considered sa fer th an mineral di e sel owin g...

WAJM 29(2) LATEST.pmd

African Journals Online (AJOL)

user1

osteocalcin and 1,25 (OH)2 Vit D3 were 1478.96 ± 771.12. µmol/L, 22.33 ± 7.42 ... ORIGINAL ARTICLE. *Department of Medicine Obafemi Awolowo University Teaching Hospitals Complex, Ile-Ife. ... patients souffrant d'insuffisance rénale chronique au stade final (IRT). MÉTHODES: .... hydroxide (duration and dosages) or.

Les leçons apprises en Argentine aident Haïti à lutter contre le choléra

International Development Research Centre (IDRC) Digital Library (Canada)

17 janv. 2011 ... À un moment où le choléra a emporté plus de 2 000 Haïtiens et menace plus de 6 500 000 autres, des mesures urgentes sont prises pour endiguer l'épidémie qui sévit dans les dix départements du pays.