WorldWideScience

Sample records for unitas viii cruise

  1. Le Ferrovie e la costruzione dell'Italia Unita

    Directory of Open Access Journals (Sweden)

    Laura Facchinelli

    2011-04-01

    Full Text Available La costruzione dell’Italia unita è strettamente legata alla storia delle ferrovie. A pochi anni dall’invenzione del treno vennero attivate alcune linee, a partire dalla Napoli-Portici (1839. Si trattava, però, di brevi tratti separati tra loro, costruiti per iniziativa dei diversi Stati.I progressi compiuti dalle ferrovie in diversi paesi d’Europa, che segnavano un rapido progresso delle vie di comunicazione terrestri, accentuarono l’arretratezza della situazione italiana. Ma si andava delineando, nelle menti più lungimiranti, l’idea di un’unità almeno territoriale della penisola, da realizzare con una rete ferroviaria: in tal modo la questione ferroviaria, nel periodo del Risorgimento, venne ad inserirsi nella formazione della coscienza nazionale.Dopo la proclamazione del Regno d’Italia, superate le precedenti divisioni in tanti diversi Stati, si diede finalmente avvio a un progetto organico di costruzione della rete ferroviaria nazionale. Dal 1861 al 1865 lo sforzo costruttivo fu notevole. Cruciale fu il ventennio dal 1865 al 1885. Nel 1865 la rete della penisola venne distribuita fra quattro grandi società.Realizzata la spina dorsale del paese, venne costruito il primo grande traforo alpino, mentre si finanziava la costruzione di oltre 6.000 km di ferrovie secondarie.Nel 1885 le linee vennero raccolte in due reti organiche disposte in senso longitudinale lungo la penisola. Nel 1905, la gestione delle ferrovie venne assunta da un’unica azienda di Stato.  

  2. Photoionization of Ar VIII

    Science.gov (United States)

    Liang, Liang; Jiang, Wen-xian; Zhou, Chao

    2017-01-01

    The photoionization cross section, energy levels and widths of 22 Rydberg series (in the autoionization region) for Na-like Ar VIII were investigated by using of R-matrix method. The relativistic distorted-wave method is used to calculate the radial functions, and QB method of Quigly-Berrington [Quigley et al. 1998] is employed to calculate the resonance levels and widths. We have identified the formant in the figure of the photoionization cross section.

  3. Groundfish Ecology Cruises

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This program involved a series of one day cruises on a commercial trawl and longline vessel. Cruises were conducted once per month from 2001-2005. The objectives of...

  4. Cruising to the Expo

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Shanghai’s 2010 World Expo is a golden opportunity to bolster China’s cruise ship industry shanghai’s cruise ship port broke a number of records in 2009.The port saw 182,000 passenger visits via international cruise liners throughout the year,an increase of 73 percent over the year before.

  5. Cruising to the Expo

    Institute of Scientific and Technical Information of China (English)

    JING XIAOLEI

    2010-01-01

    @@ Shanghai's cruise ship port broke a number of records in 2009. The port saw 182,000 passenger visits via intemational cruise liners throughout the year, an increase of 73 percent over the year before. The year saw 33 visits of cruise ships that call Shanghai home and another 47 visits from ships with different home ports, breaking previous records.

  6. Integrated Advanced Microwave Sounding Unit-A (AMSU-A). Performance Verification Report: Antenna Drive Subsystem METSAT AMSU-A2 (PN:1331200-2, SN:108)

    Science.gov (United States)

    Haapala, C.

    1999-01-01

    This is the Performance Verification Report, Antenna Drive Subassembly, Antenna Drive Subsystem, METSAT AMSU-A2 (P/N 1331200-2, SN: 108), for the Integrated Advanced Microwave Sounding Unit-A (AMSU-A).

  7. Cruise tourism shore excursions

    DEFF Research Database (Denmark)

    João Lopes, Maria; Dredge, Dianne

    2017-01-01

    Very complex yet highly integrated business logics characterise cruise tourism with shore excursions frequently identified as a key source of value. This paper presents a case study of cruise tourism and shore excursion planning in Copenhagen, Denmark. The aim of this paper is to investigate...... the characteristics of cruise tourism, itinerary and shore excursion planning with a view to understanding the value generated from cruise tourism shore excursions. We argue that economic value is a blunt measure, and there are other types of value, positive and negative, that are also generated. This research...... reveals that a range of local conditions and structural characteristics create barriers and opportunities for generating different types of value. Using a case study of shore excursions in Copenhagen, the Baltic’s most important port, this paper explains the dynamics between cruise tourism and shore...

  8. The US Cruise Ship Industry.

    Science.gov (United States)

    Miller, Willis H.

    1985-01-01

    The cruise ship industry relates directly to many features of the natural and cultural environments. The U.S. cruise ship industry is analyzed. Discusses the size of the industry, precruise passenger liners, current cruise ships, cruise regions and routes, ports of call, major ports, passengers, and future prospects. (RM)

  9. Juvenile Rockfish Recruitment Cruise

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — In 1983, the groundfish analysis project began a series of yearly cruises designed to assess the annual abundance of juvenile rockfish along the central California...

  10. Travelers' Health: Cruise Ship Travel

    Science.gov (United States)

    ... Water Disinfection Infographics for Travelers MERS Health Advisory poster Food and Water: What's Safer Health Advisory: MERS ... cruise ships can vary widely depending on ship size, itinerary, length of cruise, and passenger demographics. Generally, ...

  11. Cooperative adaptive cruise control

    NARCIS (Netherlands)

    Naus, G.J.L.; Molengraft, R. van de; Ploeg, J.

    2009-01-01

    Adaptive Cruise Control (ACC) enables automatic following of a preceding vehicle, based on measurements of the inter-vehicle distance xr,i and the relative velocity ˙ xr,i. Commonly, a radar is used for these measurements, see Figure 1. Decreasing the inter-vehicle distance to a small value of only

  12. Alfonso VIII, Cruzada y Cristiandad = Alfonso VIII, Crusade and Christendom

    Directory of Open Access Journals (Sweden)

    Carlos de Ayala Martínez

    2016-06-01

    Full Text Available Lacruzada fue el cauce más directo para la integración de la Península Ibérica en el conjunto de la Cristiandad. Este hecho se pone especialmente de relieve cuando nos acercamos al reinado de Alfonso VIII de Castilla. El monarca, que casó con la hija del rey de Inglaterra, comprometió el matrimonio de su primogénita con el heredero del emperador alemán y casó a otra de sus hijas con el futuro rey de Francia, representa esa plena integración cuyo seguimiento es posible a través de diversos indicadores –políticos, ideológicos, culturales…-, que, en todo caso, refuerzan la imagen propagandística de un rey al servicio de la defensa de la Cristiandad.Crusading was the most effective method of integrating the Iberian Peninsula within Christendom. This fact is most evident when we consider the reign of Alfonso VIII of Castile. This monarch married the daughter of the king of England, arranged the marriage of his eldest daughter with the heir of the German Emperor and married another of his daughters with the future king of France. These actions convey the need for maximum integration which can be traced using several indicators –political, ideological, cultural...– that moreover reinforce the propaganda image of a king at the service and in defence of Christianity.

  13. Integrated Advanced Microwave Sounding Unit-A (AMSU-A). As-Designed Parts List: Electrical, Electronic, and Electromechanical (EEE) As-Built Parts List for the AMSU-A Instruments

    Science.gov (United States)

    2000-01-01

    This is the As-Designed Parts List, Electrical, Electronic, and Electromechanical (EEE) As-Built Parts Lists For The AMSU-A Instruments, for the Integrated Advanced Microwave Sounding Unit-A (AMSU-A).

  14. Coagulation factor VIII activity in diabetic patients

    Directory of Open Access Journals (Sweden)

    Nermina Babić

    2011-02-01

    Full Text Available Aim To examine coagulation factor VIII activity in plasma, as a risk factor for thrombosis, in the patients with diabetes mellitus (DM. Also, to assess its relationship with ibrinogen and fasting blood glucose concentrations and with body mass index. Methods The plasma coagulation factor VIII activity, plasma levels of ibrinogen and blood glucose concentrations were measured in 30 patients with DM type 1, 30 patients with DM type 2 and in 30 healthy subjects. Body weight and body height were also measured and BMI was calculated.Results The plasma factor VIII activity in patients with DM type 1 and patients with DM type 2 was signiicantly higher than the values measured in healthy subjects. There was no signiicant difference in the factor VIII activity between patients with DM type 1 and type 2. The concentrations of ibrinogen and blood glucose in both groups of patients were signiicantly higher than in the group of healthy subjects. Patients with DM type 2 had a signiicantly higher BMI compared to healthy subjects, as well as compared to patients with DM type 1. There was a signiicant positive correlation between plasma factor VIII activity and plasma level of ibrinogen and a signiicant negative correlation between factor VIII activity and BMI in patients with DM type 2. Conclusion Diabetic patients have the elevated plasma coagulation factor VIII activity and increased ibrinogen concentration thus an increased risk of thrombosis and vascular diseases.

  15. Attacking the Cruise Missile Threat

    Science.gov (United States)

    2010-07-26

    little warning before a strike. Cruise missiles such as Silkworm and Exocet are designed to fly at “sea-skimming” levels only a few feet above the surface...the SS-N-2 Styx missile, a surface-to-surface antiship cruise missile. Most variants of modern cruise missiles are conversions of the Styx—the Silkworm ...number of Soviet built SS-N-2 Styxes that had been dismounted from boats.23 The Silkworm , the predominant of these variants, is the “Scud” of cruise

  16. 12 CFR 611.1137 - Title VIII service corporations.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 6 2010-01-01 2010-01-01 false Title VIII service corporations. 611.1137... Organizations § 611.1137 Title VIII service corporations. (a) What is a title VIII service corporation? A title VIII service corporation is a service corporation organized for the purpose of exercising...

  17. Norovirus transmission on cruise ship.

    Science.gov (United States)

    Isakbaeva, Elmira T; Widdowson, Marc-Alain; Beard, R Suzanne; Bulens, Sandra N; Mullins, James; Monroe, Stephan S; Bresee, Joseph; Sassano, Patricia; Cramer, Elaine H; Glass, Roger I

    2005-01-01

    An outbreak of norovirus gastroenteritis affected passengers on two consecutive cruises of ship X and continued on 4 subsequent cruises despite a 1-week sanitization. We documented transmission by food and person-to-person contact; persistence of virus despite sanitization onboard, including introductions of new strains; and seeding of an outbreak on land.

  18. Oceanographic Mower Cruise

    Science.gov (United States)

    Valencia, J.; Ercilla, G.; Hernández-Molina, F. J.; Casas, D.

    2015-04-01

    The MOWER Cruise has executed a geophysics and geologic expedition in the Gulf of Cádiz (sector adjacent to the Strait of Gibraltar) and west off Portugal, in the framework of the coordinate research project MOWER "Erosive features and associated sandy deposits generated by the Mediterranean Outflow Water (MOW) around Iberia: paleoceanographic, sedimentary & economic implications" (CTM 2012-39599-C03). The main aim of this project is to identify and study the erosional features (terraces and channels) and associated sedimentary deposits (sandy contourites) generated by the Mediterranean Water Masses around the middle continental slope of Iberia (The Mediterranean Outflow Water - MOW - in the Atlantic margins), their Pliocene and Quaternary evolution and their paleoceanographic, sedimentary and economic implications. This objective directly involves the study of alongslope (contourite) processes associated with the MOW and across-slope (turbiditic flows, debris flows, etc.) processes in the sedimentary stacking pattern and evolution of the Iberian margins. The MOWER project and cruise are related to the Integrated Ocean Drilling Program (IODP) Expedition 339 (Mediterranean Outflow). It is also linked and coordinated with CONDRIBER Project "Contourite drifts and associated mass-transport deposits along the SW Iberia margin - implications to slope stability and tsunami hazard assessment" (2013-2015) funded by the Fundação para a Ciência e Tecnologia, Portugal (PTDC/GEO-GEO/4430/2012).

  19. CMO: Cruise Metadata Organizer for JAMSTEC Research Cruises

    Science.gov (United States)

    Fukuda, K.; Saito, H.; Hanafusa, Y.; Vanroosebeke, A.; Kitayama, T.

    2011-12-01

    JAMSTEC's Data Research Center for Marine-Earth Sciences manages and distributes a wide variety of observational data and samples obtained from JAMSTEC research vessels and deep sea submersibles. Generally, metadata are essential to identify data and samples were obtained. In JAMSTEC, cruise metadata include cruise information such as cruise ID, name of vessel, research theme, and diving information such as dive number, name of submersible and position of diving point. They are submitted by chief scientists of research cruises in the Microsoft Excel° spreadsheet format, and registered into a data management database to confirm receipt of observational data files, cruise summaries, and cruise reports. The cruise metadata are also published via "JAMSTEC Data Site for Research Cruises" within two months after end of cruise. Furthermore, these metadata are distributed with observational data, images and samples via several data and sample distribution websites after a publication moratorium period. However, there are two operational issues in the metadata publishing process. One is that duplication efforts and asynchronous metadata across multiple distribution websites due to manual metadata entry into individual websites by administrators. The other is that differential data types or representation of metadata in each website. To solve those problems, we have developed a cruise metadata organizer (CMO) which allows cruise metadata to be connected from the data management database to several distribution websites. CMO is comprised of three components: an Extensible Markup Language (XML) database, an Enterprise Application Integration (EAI) software, and a web-based interface. The XML database is used because of its flexibility for any change of metadata. Daily differential uptake of metadata from the data management database to the XML database is automatically processed via the EAI software. Some metadata are entered into the XML database using the web

  20. Drague et cruising

    Directory of Open Access Journals (Sweden)

    Emmanuel Redoutey

    2008-04-01

    Full Text Available Dans la culture homosexuelle masculine, drague et cruising sont des termes équivalents. Ils désignent la quête d’un ou de plusieurs partenaires occasionnels et anonymes. Analyser leurs référents métaphoriques respectifs offre un éclairage particulier, à la fois phénoménologique et géographique, pour une compréhension nuancée des types de pratique et d’expérience qu’ils recouvrent. Cet article pose l’hypothèse d’une distinction entre deux figures, le dragueur et le cruiser, et postule que cette distinction se tient essentiellement dans l’opposition que Gilles Deleuze et Félix Guattari établissent entre ‘espace strié’ et ‘espace lisse’. L’essai de théorisation qui en découle est une manière de comprendre ce qui, entre sexualisation de l’espace et érotisation d’un mouvement exploratoire, fait fonctionner le script de la drague.In French gay culture, drague means cruising: looking for anonymous and 
casual sexual partners. This paper, by respectively examining the 
metaphorical underpinnings of both words, French and English, throws 
doubt on the validity of this translation. Through a phenomenological 
and geographical perspective, it attempts to give a nuanced examination 
of the practice and experience that each word conceals. The aim is to 
identify two figures embodied in a same person: the dragueur and the 
cruiser. I will argue that the distinction mainly rests on the 
opposition that Gilles Deleuze and Felix Guattari establish between ‘striated space’ and ‘smooth space’. The concluding theorical 
discussion is an attempt to understand what, in the tension between sexualization of space and eroticization of movement, guides the scripts of drague and cruising.

  1. Developmental Continuity? Crawling, Cruising, and Walking

    Science.gov (United States)

    Adolph, Karen E.; Berger, Sarah E.; Leo, Andrew J.

    2011-01-01

    This research examined developmental continuity between "cruising" (moving sideways holding onto furniture for support) and walking. Because cruising and walking involve locomotion in an upright posture, researchers have assumed that cruising is functionally related to walking. Study 1 showed that most infants crawl and cruise concurrently prior…

  2. Cruise Report RV Poseidon Cruise No. P 475 [POS475

    OpenAIRE

    Leipe, Thomas

    2014-01-01

    Dates of the cruise: from 27. 9. to 12. 10. 2014. - Purpose of the cruise: Investigation of transport, deposition and exchange of matter at the sediment-water boundary in German waters (Project SECOS). Reconstruction of Holocene history of the western Baltic Sea (Gdansk-Basin and Bornholm Basin). Acoustic mapping of the sea floor at selected areas in German waters. Documentation and sampling of “stone reef habitats” by research scuba divers (German waters only).

  3. Post-Cruise Questionnaire - Legacy

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Post-Cruise Questionnaire is a mandatory post trip legal document that observers fill out after every trip they have completed.

  4. Post-Cruise Questionnaire - Legacy

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Post-Cruise Questionnaire is a mandatory post trip legal document that observers fill out after every trip they have completed.

  5. Blood coagulation factor VIII: An overview

    Indian Academy of Sciences (India)

    G M Bhopale; R K Nanda

    2003-12-01

    Factor VIII (FVIII) functions as a co-factor in the blood coagulation cascade for the proteolytic activation of factor X by factor IXa. Deficiency of FVIII causes hemophilia A, the most commonly inherited bleeding disorder. This review highlights current knowledge on selected aspects of FVIII in which both the scientist and the clinician should be interested.

  6. Game, set, match for factor VIII mismatch?

    Science.gov (United States)

    Miller, Connie H

    2015-08-13

    In this issue of Blood, Gunasekera et al provide evidence that the high rate of factor VIII (FVIII) inhibitors seen in black hemophilia A (HA) patients is not due to a mismatch between the structure of treatment products and FVIII genotypes common in blacks.

  7. Meteorological Satellites (METSAT) and Earth Observing System (EOS) Advanced Microwave Sounding Unit-A (AMSU-A) Failure Modes and Effects Analysis (FMEA) and Critical Items List (CIL)

    Science.gov (United States)

    1996-01-01

    This Failure Modes and Effects Analysis (FMEA) is for the Advanced Microwave Sounding Unit-A (AMSU-A) instruments that are being designed and manufactured for the Meteorological Satellites Project (METSAT) and the Earth Observing System (EOS) integrated programs. The FMEA analyzes the design of the METSAT and EOS instruments as they currently exist. This FMEA is intended to identify METSAT and EOS failure modes and their effect on spacecraft-instrument and instrument-component interfaces. The prime objective of this FMEA is to identify potential catastrophic and critical failures so that susceptibility to the failures and their effects can be eliminated from the METSAT/EOS instruments.

  8. Development and characterization of recombinant ovine coagulation factor VIII.

    Science.gov (United States)

    Zakas, Philip M; Gangadharan, Bagirath; Almeida-Porada, Graca; Porada, Christopher D; Spencer, H Trent; Doering, Christopher B

    2012-01-01

    Animal models of the bleeding disorder, hemophilia A, have been an integral component of the biopharmaceutical development process and have facilitated the development of recombinant coagulation factor VIII (fVIII) products capable of restoring median survival of persons with hemophilia A to that of the general population. However, there remain several limitations to recombinant fVIII as a biotherapeutic, including invasiveness of intravenous infusion, short half-life, immunogenicity, and lack of availability to the majority of the world's population. The recently described ovine model of hemophilia A is the largest and most accurate phenocopy. Affected sheep die prematurely due to bleeding-related pathogenesis and display robust adaptive humoral immunity to non-ovine fVIII. Herein, we describe the development and characterization of recombinant ovine fVIII (ofVIII) to support further the utility of the ovine hemophilia A model. Full-length and B-domain deleted (BDD) ofVIII cDNAs were generated and demonstrated to facilitate greater biosynthetic rates than their human fVIII counterparts while both BDD constructs showed greater expression rates than the same-species full-length versions. A top recombinant BDD ofVIII producing baby hamster kidney clone was identified and used to biosynthesize raw material for purification and biochemical characterization. Highly purified recombinant BDD ofVIII preparations possess a specific activity nearly 2-fold higher than recombinant BDD human fVIII and display a differential glycosylation pattern. However, binding to the carrier protein, von Willebrand factor, which is critical for stability of fVIII in circulation, is indistinguishable. Decay of thrombin-activated ofVIIIa is 2-fold slower than human fVIII indicating greater intrinsic stability. Furthermore, intravenous administration of ofVIII effectively reverses the bleeding phenotype in the murine model of hemophilia A. Recombinant ofVIII should facilitate the maintenance of

  9. Development and characterization of recombinant ovine coagulation factor VIII.

    Directory of Open Access Journals (Sweden)

    Philip M Zakas

    Full Text Available Animal models of the bleeding disorder, hemophilia A, have been an integral component of the biopharmaceutical development process and have facilitated the development of recombinant coagulation factor VIII (fVIII products capable of restoring median survival of persons with hemophilia A to that of the general population. However, there remain several limitations to recombinant fVIII as a biotherapeutic, including invasiveness of intravenous infusion, short half-life, immunogenicity, and lack of availability to the majority of the world's population. The recently described ovine model of hemophilia A is the largest and most accurate phenocopy. Affected sheep die prematurely due to bleeding-related pathogenesis and display robust adaptive humoral immunity to non-ovine fVIII. Herein, we describe the development and characterization of recombinant ovine fVIII (ofVIII to support further the utility of the ovine hemophilia A model. Full-length and B-domain deleted (BDD ofVIII cDNAs were generated and demonstrated to facilitate greater biosynthetic rates than their human fVIII counterparts while both BDD constructs showed greater expression rates than the same-species full-length versions. A top recombinant BDD ofVIII producing baby hamster kidney clone was identified and used to biosynthesize raw material for purification and biochemical characterization. Highly purified recombinant BDD ofVIII preparations possess a specific activity nearly 2-fold higher than recombinant BDD human fVIII and display a differential glycosylation pattern. However, binding to the carrier protein, von Willebrand factor, which is critical for stability of fVIII in circulation, is indistinguishable. Decay of thrombin-activated ofVIIIa is 2-fold slower than human fVIII indicating greater intrinsic stability. Furthermore, intravenous administration of ofVIII effectively reverses the bleeding phenotype in the murine model of hemophilia A. Recombinant ofVIII should facilitate

  10. Acquired Factor VIII Inhibitors: Three Cases

    Directory of Open Access Journals (Sweden)

    Tay Za Kyaw

    2013-03-01

    Full Text Available Acquired hemophilia A is a rare, but devastating bleeding disorder caused by spontaneous development of autoantibodies directed against coagulation factor VIII. In 40%-50% of patients it is associated with such conditions as the postpartum period, malignancy, use of medications, and autoimmune diseases; however, its cause is unknown in most cases. Acquired hemophilia A should be suspected in patients that present with a coagulation abnormality, and a negative personal and family history of bleeding. Herein we report 3 patients with acquired hemophilia A that had different underlying pathologies, clinical presentations, and therapeutic responses. Factor VIII inhibitor formation in case 1 occurred 6 months after giving birth; underlying disorders were not identified in cases 2 or 3. The bleeding phenotype in these patients’ ranged from no bleeding tendency with isolated prolongation of APTT (activated partial thromboplastin time to severe intramuscular hematoma and hemarthrosis necessitating recombinant activated factor VII infusion and blood components transfusion. Variable responses to immunosuppressive treatment were also observed.

  11. 77 FR 50511 - Fees for Sanitation Inspections of Cruise Ships

    Science.gov (United States)

    2012-08-21

    ... 1970s as a cooperative activity with the cruise ship industry. VSP helps the cruise ship industry... October 1, 2006. The cruise ship industry should be aware that if travel expenses for VSP increase,...

  12. Integrated Advanced Microwave Sounding Unit-A (AMSU-A). As-Designed Parts List: Electrical, Electronic and Electromechanical (EEE) As-Designed Parts List

    Science.gov (United States)

    Lorenz, E.

    1999-01-01

    This report comprises the Electrical, Electronic, and Electromechanical (EEE) As Designed Parts List to be used in the Integrated Advanced Microwave Sounding Unit-A (AMSU-A) instrument. The purpose of the EEE As-Designed Parts List is to provide a listing of EEE parts identified for use on the Integrated AMSU-A. All EEE parts used on the AMSU-A must meet the parts control requirements as defined in the Parts Control Plan (POP). All part applications are reviewed by the Parts Control Board (PCB) and granted approval if POP requirements are met. The "As Designed Parts Lists" indicates PCB approval status, and thus also serves as the Program Approved Parts List.

  13. Cruise ship seakeeping and passenger comfort

    NARCIS (Netherlands)

    Dallinga, R.P.; Bos, J.E.

    2010-01-01

    In the last decade the design and construction of a substantial volume of large cruise ships has stimulated the evolution of cruise ship design. The MARIN and TNO involvement in these developments, and in particular the increase in size, have lead to new insights in how the ship design governs passe

  14. Continuous infusion of factor VIII for surgery and major bleeding.

    Science.gov (United States)

    Hay, C R; Doughty, H I; Savidge, G F

    1996-03-01

    In a clinical trial, 24 patients with haemophilia A who needed surgery or had suffered severe bleeding were treated by continuous infusion of Monoclate P, a factor VIII concentrate that is immunopurified by monoclonal antibodies. Continuous infusion of Monoclate P began with a dose of 2 U/kg per h that was adjusted according to the results of factor VIII assays to achieve a factor VIII target level of 100 IU/dl for 2 days and then 80 IU/dl for 5 days. The safety, efficacy, and economics of this approach were assessed. No haemorrhagic episodes were observed. The continuous infusion was convenient and had the advantage of producing steady-state levels of factor VIII. With a single-compartment model, we found median factor VIII clearance values of 3.11 (range 1.79-7.78) x 10(3) litres/kg per h, elimination rates of 5.0-19.4 x 10(-2)/h and a median half-life of 9.9 h (range 4.8-20.0 h). Clearance and the elimination rate appeared to decline over the infusion period, as judged by the decreasing infusion rate required to maintain the target concentration of factor VIII. An economic comparison with bolus therapy, using theoretically derived bolus dosages, indicated that the potential saving was related inversely to the factor VIII half-life. Potential savings of 75% were predicted on the first postoperative day, averaging 35% over the full course of therapy.

  15. Cruise Ship Plume Tracking Survey Report

    Science.gov (United States)

    2002-09-01

    The U. S. Environmental Protection Agency (EPA) is developing a Cruise Ship Discharge Assessment Report in response to a petition the agency received in March 2000. The petition requested that EPA assess and where necessary control discharges from cruise ships. Comments received during public hearings, in 2000, resulted in the EPA agreeing to conduct a survey to assess the discharge plumes resulting from cruise ships, operating in ocean waters off the Florida coast and to compare the results to the Alaska dispersion models. This survey report describes the daily activities of August 2001 Cruise Ship Plume Tracking Survey, and provides a synopsis of the observations from the survey. It also provides data that can be used to assess dispersion of cruise ship wastewater discharges, while in transit. A description of the survey methods is provided in Section 2. Survey results are presented in Section 3. Findings and conclusions are discussed in Section 4.

  16. Prey detection in a cruising copepod

    DEFF Research Database (Denmark)

    Kjellerup, Sanne; Kiørboe, Thomas

    2012-01-01

    Small cruising zooplankton depend on remote prey detection and active prey capture for efficient feeding. Direct, passive interception of prey is inherently very inefficient at low Reynolds numbers because the viscous boundary layer surrounding the approaching predator will push away potential prey....... Yet, direct interception has been proposed to explain how rapidly cruising, blind copepods feed on non-motile phytoplankton prey. Here, we demonstrate a novel mechanism for prey detection in a cruising copepod, and describe how motile and non-motile prey are discovered by hydromechanical and tactile...

  17. Prey detection in a cruising copepod

    DEFF Research Database (Denmark)

    Kjellerup, Sanne; Kiørboe, Thomas

    2012-01-01

    Small cruising zooplankton depend on remote prey detection and active prey capture for efficient feeding. Direct, passive interception of prey is inherently very inefficient at low Reynolds numbers because the viscous boundary layer surrounding the approaching predator will push away potential prey....... Yet, direct interception has been proposed to explain how rapidly cruising, blind copepods feed on non-motile phytoplankton prey. Here, we demonstrate a novel mechanism for prey detection in a cruising copepod, and describe how motile and non-motile prey are discovered by hydromechanical and tactile...

  18. A "broken heart" on a cruise ship.

    Science.gov (United States)

    Taylor, Christopher

    2015-01-01

    An elderly female cruise ship passenger developed chest pain shortly after an emotionally charged incident involving shipboard authorities. The electrocardiogram and cardiac enzyme profile were indicative of myocardial infarction but the final diagnosis, established after aeromedical evacuation, was stress-related cardiomyopathy. This case is an example of a relatively unknown clinical entity that is easily mistaken for acute myocardial infarction and which may be disproportionately prevalent in the cruise ship passenger population.

  19. Staphylococcal food poisoning on a cruise ship.

    OpenAIRE

    Waterman, S H; Demarcus, T. A.; Wells, J G; Blake, P A

    1987-01-01

    Two waves of vomiting and/or diarrhoea affected approximately 215 of the 715 passengers on a Caribbean cruise ship. The outbreak was independently associated with eating cream-filled pastries at two separate meals. Staphylococcus aureus phage type 85/+ was isolated from cases and pastry cooks, but not from controls. This is the first well-documented outbreak of staphylococcal food poisoning on a cruise ship.

  20. Autonomous intelligent cruise control system

    Science.gov (United States)

    Baret, Marc; Bomer, Thierry T.; Calesse, C.; Dudych, L.; L'Hoist, P.

    1995-01-01

    Autonomous intelligent cruise control (AICC) systems are not only controlling vehicles' speed but acting on the throttle and eventually on the brakes they could automatically maintain the relative speed and distance between two vehicles in the same lane. And more than just for comfort it appears that these new systems should improve the safety on highways. By applying a technique issued from the space research carried out by MATRA, a sensor based on a charge coupled device (CCD) was designed to acquire the reflected light on standard-mounted car reflectors of pulsed laser diodes emission. The CCD is working in a unique mode called flash during transfer (FDT) which allows identification of target patterns in severe optical environments. It provides high accuracy for distance and angular position of targets. The absence of moving mechanical parts ensures high reliability for this sensor. The large field of view and the high measurement rate give a global situation assessment and a short reaction time. Then, tracking and filtering algorithms have been developed in order to select the target, on which the equipped vehicle determines its safety distance and speed, taking into account its maneuvering and the behaviors of other vehicles.

  1. Idiopathic Acquired Hemophilia A with Undetectable Factor VIII Inhibitor

    Directory of Open Access Journals (Sweden)

    Nicholas B. Abt

    2014-01-01

    Full Text Available Objective. We present the case of a 73-year-old female, with no family or personal history of a bleeding disorder, who had a classic presentation for acquired hemophilia A. Factor VIII activity was low but detectable and a factor VIII inhibitor was undetectable. Methods. The patient’s plasma was comprehensively studied to determine the cause of the acquired coagulopathy. Using the Nijmegen modification of the Bethesda assay, no factor VIII autoantibody was measureable despite varying the incubation time from 1 to 3 hours. Results. The aPTT was prolonged at 46.8 seconds, which did not correct in the 4 : 1 mix but did with 1 : 1 mix. Using a one stage factor VIII activity assay, the FVIII activity was 16% and chromogenic FVIII activity was also 16%. The patient was treated with recombinant FVII and transfusion, significantly reducing bleeding. Long-term therapy was initiated with cyclophosphamide and prednisone with normalization of FVIII activity. Conclusions. Physicians can be presented with the challenging clinical picture of an acquired factor VIII inhibitor without a detectable inhibitor by the Bethesda assay. Standard therapy for an acquired hemophilia A should be considered.

  2. Wake-Vortex Hazards During Cruise

    Science.gov (United States)

    Rossow, Vernon J.; James, Kevin D.; Nixon, David (Technical Monitor)

    1998-01-01

    Even though the hazard posed by lift-generated wakes of subsonic transport aircraft has been studied extensively for approach and departure at airports, only a small amount of effort has gone into the potential hazard at cruise altitude. This paper reports on a studio of the wake-vortex hazard during cruise because encounters may become more prevalent when free-flight becomes available and each aircraft, is free to choose its own route between destinations. In order to address the problem, the various fluid-dynamic stages that vortex wakes usually go through as they age will be described along with estimates of the potential hazard that each stage poses. It appears that a rolling-moment hazard can be just as severe at cruise as for approach at airports, but it only persists for several minutes. However, the hazard posed by the downwash in the wake due to the lift on the generator aircraft persists for tens of minutes in a long narrow region behind the generating aircraft. The hazard consists of severe vertical loads when an encountering aircraft crosses the wake. A technique for avoiding vortex wakes at cruise altitude will be described. To date the hazard posed by lift-generated vortex wakes and their persistence at cruise altitudes has been identified and subdivided into several tasks. Analyses of the loads to be encounter and are underway and should be completed shortly. A review of published literature on the subject has been nearly completed (see text) and photographs of vortex wakes at cruise altitudes have been taken and the various stages of decay have been identified. It remains to study and sort the photographs for those that best illustrate the various stages of decay after they are shed by subsonic transport aircraft at cruise altitudes. The present status of the analysis and the paper are described.

  3. Integrated Advanced Microwave Sounding Unit-A (AMSU-A). Performance Verification Report: AMSU-A1 Antenna Drive Subsystem, PN 1331720-2, S/N 106

    Science.gov (United States)

    Luu, D.

    1999-01-01

    This is the Performance Verification Report, AMSU-A1 Antenna Drive Subsystem, P/N 1331720-2, S/N 106, for the Integrated Advanced Microwave Sounding Unit-A (AMSU-A). The antenna drive subsystem of the METSAT AMSU-A1, S/N 106, P/N 1331720-2, completed acceptance testing per A-ES Test Procedure AE-26002/lD. The test included: Scan Motion and Jitter, Pulse Load Bus Peak Current and Rise Time, Resolver Reading and Position Error, Gain/ Phase Margin, and Operational Gain Margin. The drive motors and electronic circuitry were also tested at the component level. The drive motor test includes: Starting Torque Test, Motor Commutation Test, Resolver Operation/ No-Load Speed Test, and Random Vibration. The electronic circuitry was tested at the Circuit Card Assembly (CCA) level of production; each test exercised all circuit functions. The transistor assembly was tested during the W3 cable assembly (1356941-1) test.

  4. RRS "Charles Darwin" Cruise CD170 and RV "Knorr" Cruise KN182-2. RAPID mooring cruise report April - May 2005

    OpenAIRE

    S. A. Cunningham; D. Rayner

    2006-01-01

    This report describes the mooring operations conducted during RRS Charles Darwin Cruise CD170 and RV Knorr Cruise KN182-2. Cruise CD170 was conducted between 2 April 2005 and 27 April 2005, and Cruise KN182-2 was conducted between 2 May 2005 and 26 May 2005.\\ud \\ud These cruises were completed as part of the United Kingdom Natural Environment Research Council (NERC) funded RAPID Programme and the United States of America National Science Federation (NSF) funded MOCHA Programme to monitor the ...

  5. The Subduction Experiment, Cruise Report, RRS Charles Darwin Cruise Number 73. Subduction 3 Mooring Deployment and Recovery Cruise 30 September-26 October 1992

    Science.gov (United States)

    1993-03-01

    RRS Charles Darwin Cruise Number 73 Subduction 3 Mooring Deployment and Recovery Cruise 30 September -26 October 1992 * by T Richard P. Trask 1.7 i•; A...RRS Charles Darwin cruise number 73 which was the third scheduled Subduction mooring cruise. During this cruise the second setting of the moorings...14 Table A6-1 XBT Positions 82 I S "I U Im ’i i i n I Section 1: Introduction The RRS Charles Darwin departed Funchal, Madeira at 0910 UTC on

  6. Prospect of Cruising in Boka Kotorska

    Directory of Open Access Journals (Sweden)

    Đurđica Perović

    2013-01-01

    Full Text Available The main objectives of this research are to get answers, to the following: the achieved level of development of cruise tourism in the Bay of Kotor; characteristics of cruise tourism in Kotor, what are the possibilities of innovation in the cruise tourism in the port of Kotor, how to adjust the cruise tourism sustainable development of Kotor, as a tourist destination? Merely by being a part of the Mediterranean, which represents a developing market in cruising, Boka Kotorska has endless potential. Yet, its significance is still to be recognized only if careful strategy of further development is followed. Having in mind that Boka Kotorska is a valuable natural and cultural heritage site, it is necessary to make assessments in terms of sustainability of this kind of tourism as well as actions that should be taken. Its limited geographical area, more suitable for minor boats and not ships, is something that should be taken into account. Other things such as tourist offer enrichment in the Old Town, including not just the seaside but background of Boka Kotorska in the tourist offer, would certainly improve the quality of tourist product. This would undoubtedly generate more profit.

  7. Cardiovascular emergencies in cruise ship passengers.

    Science.gov (United States)

    Novaro, Gian M; Bush, Howard S; Fromkin, Kenneth R; Shen, Michael Y; Helguera, Marcelo; Pinski, Sergio L; Asher, Craig R

    2010-01-15

    More than 10 million people, many elderly and likely to harbor cardiovascular (CV) disease, embark on cruise ship travel worldwide every year. The clinical presentation and outcome of CV emergencies presenting during cruise ship travel remain largely unknown. Our department provides contracted cardiology consultations to several large cruise lines. We prospectively maintained a registry of all such consultations during a 2-year period. One hundred consecutive patients were identified (age 66 +/- 14 years, range 18 to 90, 76% men). The most common symptom was chest pain (50%). The most common diagnosis was acute coronary syndrome (58%; ST elevation in 21% and non-ST elevation in 37%). On-board mortality was 3%. Overall, 73% of patients required hospital triage. Of the 25 patients triaged to our institution, 17 underwent a revascularization procedure. One patient died. Ten percent of patients had cardiac symptoms in the days or weeks before boarding; all required hospital triage. Access to a baseline electrocardiogram would have been clinically useful in 23% of cases. In conclusion, CV emergencies, such as acute coronary syndrome and heart failure, are not uncommon on cruise ships. They are often serious, requiring hospital triage and coronary revascularization. A pretravel medical evaluation is recommended for passengers with a cardiac history or a high-risk profile. Passengers should be encouraged to bring a copy of their electrocardiogram on board if abnormal. Cruise lines should establish mechanisms for prompt consultation and triage.

  8. Isolation, subunit structure, and proteolytic modification of bovine factor VIII.

    Science.gov (United States)

    Legaz, M E; Weinstein, M J; Heldebrant, C M; Davie, E W

    1975-01-20

    A new method has been described for the isolation of factor VIII. The method results in a high yield of factor VIII that is homogeneous by several different criteria. The purified protein is very stable and is not dissociated in the presence of 1 M NaCl or 0.25 M CaCl2. The highly purified protein is readily activated and inactivated by various proteolytic enzymes, such as thrombin, plasmin, and trypsin. The molecular events that lead to the activation reaction, however, have not been established.

  9. Presentation and management of a stage Ia lung cancer patient with a paraneoplastic factor VIII inhibitor.

    Science.gov (United States)

    Laselle, Brooks T; Boggio, Lisa N; Blum, Matthew G

    2006-01-01

    Acquired inhibitors to clotting factors most commonly involve factor VIII and are associated with autoimmune disease. Factor VIII inhibitors can cause severe spontaneous and iatrogenic bleeding that is difficult to manage. Factor VIII inhibitors are rarely associated with solid tumors and only three cases of adenocarcinoma of the lung have been reported. This report describes the multidisciplinary management of a factor VIII inhibitor-producing stage Ia lung adenocarcinoma that ultimately resulted in complete resectability.

  10. Customer Relationship Management in Asia/Pacific Cruise Industry

    OpenAIRE

    Liu, Xiao Meng

    2006-01-01

    ABSTRACT This study has demonstrated Customer Relationship Management (CRM) in the cruise industry is vital for its long-term success. The globalization of business has directly influenced the overall business strategy of cruise companies worldwide. Many cruise companies are consolidating into a few large corporations through merger and acquisition. These big cruise companies are extending their businesses to every corner of the world, with increasing bigger ships providing high volume pa...

  11. Optimal speed of hypersonic cruise flight

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    A coupling frame of speed gain and maintain was suggested to assess the flight performance of hypersonic cruise vehicles(HCV).The optimal cruise speed was obtained by analyzing the flight performance measured by the ratio of initial boost mass to generalized payload.The performance of HCVs based on rockets and air-breathing ramjets was studied and compared to that of a minimum-energy ballistic trajectory under a certain flight distance.It is concluded that rocket-based HCVs flying at the optimal speed ar...

  12. Ervaringen met Advanced Cruise Control (ACC) in een korte praktijkproef.

    NARCIS (Netherlands)

    Oei, H.-l.

    2003-01-01

    Experiences with Advanced Cruise Control in traffic; a limited experiment. Advanced Cruise Control (ACC) is an ordinary cruise control in which the desired speed is installed manually, but in which the headway time to the vehicle in front is also taken into account. If the headway time becomes less

  13. 16 CFR 309.22 - Determining estimated cruising range.

    Science.gov (United States)

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Determining estimated cruising range. 309.22... Fueled Vehicles § 309.22 Determining estimated cruising range. (a) Dedicated vehicles. (1) Estimated cruising range values for dedicated vehicles required to comply with the provisions of 40 CFR part 600...

  14. SWOT Amalysis on the Development of Cruise Tourism in Qingdao

    Institute of Scientific and Technical Information of China (English)

    王森

    2015-01-01

    As the cruise tourism develops prosperously in Qingdao,large numbers of problems exist.This paper researches the current situations of the cruise tourism in Qingdao and uses SWOT analysis to analyze the strengths, weaknesses, opportunities and threats for the development of cruise tourism in Qingdao.

  15. Igor Zabel 14. VIII 1958 - 23. VII 2005

    Index Scriptorium Estoniae

    2005-01-01

    Igor Zabel oli Ida-Euroopa tuntumaid kunstiteoreetikuid ja kuraatoreid. Töötas 1986. aastast Ljubljana moodsa kunsti muuseumi kuraatorina. 9. VIII avatakse seal viimane I. Zabeli kureeritud näitus "Territories, Identities, Nets-Slovene Art 1995-2005"

  16. Functional regions in coagulation factor VIII explored by mass spectrometry

    NARCIS (Netherlands)

    Bloem, E.

    2013-01-01

    The molecular mechanisms behind the function of factor VIII (FVIII) have remained poorly understood. FVIII acts in the blood coagulation cascade as cofactor for activated factor IX (FIXa) in the membrane bound activated factor X generating (FXase) complex. A functional absence in FVIII leads to the

  17. Comparison of factor VIII transgenes bioengineered for improved expression in gene therapy of hemophilia A.

    Science.gov (United States)

    Dooriss, Kerry L; Denning, Gabriela; Gangadharan, Bagirath; Javazon, Elisabeth H; McCarty, David A; Spencer, H Trent; Doering, Christopher B

    2009-05-01

    Successful gene therapy of hemophilia A depends on the sustained expression of therapeutic levels of factor VIII (fVIII). Because of mRNA instability, interactions with resident endoplasmic reticulum (ER) chaperones, and the requirement for carbohydrate-facilitated transport from the ER to the Golgi apparatus, fVIII is expressed at much lower levels from mammalian cells than other proteins of similar size and complexity. A number of bioengineered forms of B domain-deleted (BDD) human fVIII have been generated and shown to have enhanced expression. Previously, we demonstrated that recombinant BDD porcine fVIII exhibits high-level expression due to specific sequence elements that increase biosynthesis via enhanced posttranslational transit through the secretory pathway. In the current study, high-expression recombinant fVIII constructs were compared directly in order to determine the relative expression of the various bioengineered fVIII transgenes. The data demonstrate that BDD porcine fVIII expression is superior to that of any of the human fVIII variant constructs tested. Mean fVIII expression of 18 units/10(6) cells/24 hr was observed from HEK-293 cells expressing a single copy of the porcine fVIII transgene, which was 36- to 225-fold greater than that of any human fVIII transgene tested. Furthermore, greater than 10-fold higher expression was observed in human cells transduced with BDD porcine fVIII versus BDD human fVIII-encoding lentiviral vectors, even at low proviral copy numbers, supporting its use over other human fVIII variants in future hemophilia A gene therapy clinical trials.

  18. CRUISE FUZZY CONTROL FOR AUTOMOBILE WITH CVT

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    To develop cruise control system of an automobile with the metal pushing V-belt type CVT, the dynamic model of automobile travelling longitudinally is established, and the fuzzy controller of control system is designed. Considering uncertainty system parameter and exterior resistance disturbances, the stability of controller is investigated by simulating. The results of its simulation show that the fuzzy controller designed has practicability.

  19. Hydrographic data from the GEF Patagonia cruises

    Directory of Open Access Journals (Sweden)

    M. Charo

    2014-02-01

    Full Text Available The hydrographic data reported here were collected within the framework of the Coastal Contamination, Prevention and Marine Management project (GEF Patagonia, which was part of the scientific agenda of the United Nations Development Program (UNDP. The project goal was to strengthen efforts to improve sustainable management of marine biodiversity and reduce pollution of the Patagonia marine environment. The observational component of the project included three multi-disciplinary oceanographic cruises designed to improve the knowledge base of the marine environment and to determine the seasonal variability of physical, biological and chemical properties of highly productive regions in the southwest South Atlantic continental shelf. The cruises were carried out on board R/V Ara Puerto Deseado, in October 2005 and March and September 2006. In each cruise, hydrographic stations were occupied along cross-shelf sections spanning the shelf from near-shore to the western boundary currents between 38° and 55° S. This paper reports the quasi-continuous vertical profiles (CTD and underway surface temperature and salinity data collected during the GEF Patagonia cruises. These data sets are available at the National Oceanographic Data Center, NOAA. US, doi:10.7289/V5RN35S0.

  20. Masterplan Wind - Seabirds Cruise Report January 2011

    NARCIS (Netherlands)

    Witte, R.H.; Witte, L.

    2011-01-01

    This cruise report presents the seabird and marine mammal data collected during the 10th ‘fish egg and fish larvae’ survey on the Dutch Continental Shelf, in a series of 12 monthly surveys from April 2010 till March 2011.

  1. Understanding Taxi Drivers’ Multi-day Cruising Patterns

    Directory of Open Access Journals (Sweden)

    Fang Zong

    2015-12-01

    Full Text Available This study investigates taxi drivers’ multi-day cruising behaviours with GPS data collected in Shenzhen, China. By calculating the inter-daily variability of taxi drivers’ cruising behaviours, the multi-day cruising patterns are investigated. The impacts of learning feature and habitual feature on multi-day cruising behaviours are determined. The results prove that there is variability among taxis’ day-to-day cruising behaviours, and the day-of-week pattern is that taxi drivers tend to cruise a larger area on Friday, and a rather focused area on Monday. The findings also indicate that the impacts of learning feature and habitual feature are more obvious between weekend days than among weekdays. Moreover, learning feature between two sequent weeks is found to be greater than that within one week, while the habitual feature shows recession over time. By revealing taxis' day-to-day cruising pattern and the factors influencing it, the study results provide us with crucial information in predicting taxis' multi-day cruising locations, which can be applied to simulate taxis' multi-day cruising behaviour as well as to determine the traffic volume derived from taxis' cruising behaviour. This can help us in planning of transportation facilities, such as stop stations or parking lots for taxis. Moreover, the findings can be also employed in predicting taxis' adjustments of multi-day cruising locations under the impact of traffic management strategies.

  2. Factor VIII/V C-domain swaps reveal discrete C-domain roles in factor VIII function and intracellular trafficking.

    Science.gov (United States)

    Ebberink, Eduard H T M; Bouwens, Eveline A M; Bloem, Esther; Boon-Spijker, Mariëtte; van den Biggelaar, Maartje; Voorberg, Jan; Meijer, Alexander B; Mertens, Koen

    2017-04-01

    Factor VIII C-domains are believed to have specific functions in cofactor activity and in interactions with von Willebrand factor. We have previously shown that factor VIII is co-targeted with von Willebrand factor to the Weibel-Palade bodies in blood outgrowth endothelial cells, even when factor VIII carries mutations in the light chain that are associated with defective von Willebrand factor binding. In this study, we addressed the contribution of individual factor VIII C-domains in intracellular targeting, von Willebrand factor binding and cofactor activity by factor VIII/V C-domain swapping. Blood outgrowth endothelial cells were transduced with lentivirus encoding factor V, factor VIII or YFP-tagged C-domain chimeras, and examined by confocal microscopy. The same chimeras were produced in HEK293-cells for in vitro characterization and chemical foot-printing by mass spectrometry. In contrast to factor VIII, factor V did not target to Weibel-Palade bodies. The chimeras showed reduced Weibel-Palade body targeting, suggesting that this requires the factor VIII C1-C2 region. The factor VIII/V-C1 chimera did not bind von Willebrand factor and had reduced affinity for activated factor IX, whereas the factor VIII/V-C2 chimera showed a minor reduction in von Willebrand factor binding and normal interaction with activated factor IX. This suggests that mainly the C1-domain carries factor VIII-specific features in assembly with von Willebrand factor and activated factor IX. Foot-printing analysis of the chimeras revealed increased exposure of lysine residues in the A1/C2- and C1/C2-domain interface, suggesting increased C2-domain mobility and disruption of the natural C-domain tandem pair orientation. Apparently, this affects intracellular trafficking, but not extracellular function. Copyright© Ferrata Storti Foundation.

  3. New study on the XeVIII spectrum

    Energy Technology Data Exchange (ETDEWEB)

    Gallardo, M. [Centro de Investigaciones Opticas (CIOp) Casilla de Correo 124, 1900 La Plata (Argentina)]. E-mail: gallardom@ciop.unlp.edu.ar; Raineri, M. [Centro de Investigaciones Opticas (CIOp) Casilla de Correo 124, 1900 La Plata (Argentina)]. E-mail: monicar@ciop.unlp.edu.ar; Giuliani, M. [Dto. de Fisica, Fac. de C. Exactas y Naturales, Universidad Nacional de Mar del Plata Funes 3350 (7600) Mar del Plata (Argentina); Lagorio, C. [Dto. de Fisica, Fac. de C. Exactas y Naturales, Universidad Nacional de Mar del Plata Funes 3350 (7600) Mar del Plata (Argentina); Padilla, S. [Dto. de Fisica, Fac. de C. Exactas y Naturales, Universidad Nacional de Mar del Plata Funes 3350 (7600) Mar del Plata (Argentina); Sarmiento, R. [Dto. De Fisica, Universidad del Atlantico, A.A 1890 Barranquilla (Colombia); Reyna Almandos, J.G. [Centro de Investigaciones Opticas (CIOp) Casilla de Correo 124, 1900 La Plata (Argentina)]. E-mail: jreyna@ciop.unlp.edu.ar

    2005-10-15

    Capillary light sources were used to observe the spectrum of seven-times-ionized xenon, XeVIII, in the 300-6000 A wavelength range. Twenty-eight energy levels belonging to the 5s-9s, 5d-9d, 5g-9g, 7i-10i, 9l, 5p-9p, 4f-9f, 6h-9h and 8k-10k configurations were adjusted and 38 new spectral lines were classified. The XeVIII ionization energy was determined with improved accuracy using the polarization model. The analysis was supported by Hartree-Fock calculations. The weighted oscillator strengths for all the observed spectral lines were also calculated considering the fitted values for the energy parameters.

  4. [Intein-mediated F309SfVIII ligation with enhanced secretion of its heavy chain.].

    Science.gov (United States)

    Zhu, Fu-Xiang; Liu, Ze-Long; Qu, Hui-Ge; Chi, Xiao-Yan

    2009-12-25

    Coagulation factor VIII (fVIII) is a secretion protein and plays a crucial role in the coagulation cascade. Hemophilia A resulted from deficiency of fVIII is the most common X-linked recessive bleeding disorder. Gene therapy is recognized as an attractive strategy for the eventual cure of this disease. However, the gene therapy is hampered by the big size of fVIII gene when using the most promising gene vectors, adeno-associated virus (AAV) vectors. In this study we explored the intein-mediated protein trans-splicing to deliver a Phe(309)-->Ser mutant full-length fVIII (F309SfVIII) gene by using a dual-vector system. An intein is a protein sequence embedded within a precursor protein and can excise itself through protein splicing. The F309SfVIII is proven to be beneficial to its secretion. The F309SfVIII gene was broken into heavy and light chains before Ser(1239) in B domain and fused with the coding sequences of Ssp DnaB intein respectively to construct a pair of plasmid vectors by inserting them into the pcDNA3.1 vectors. Forty-eight hours after co- or separate transfection of 293 cells, the co-transfected cell lysate showed an obvious ligated F309SfVIII protein band by Western blot with a polyclonal antibody against fVIII. The amounts of secreted F309SfVIII protein in culture supernatants and their bioactivities were (71+/-9) ng/mL and (0.38+/-0.09) IU/mL determined by ELISA and Coatest assay respectively. The supernatant from combined cells with separate transfections also displayed lower levels of F309SfVIII antigen and fVIII activity [(25+/-6) ng/mL and (0.12+/-0.05) IU/mL], indicating the F309SfVIII could be formed by splicing both before and after secretion. The content of F309SfVIII heavy chain protein from co-transfected cell supernatant was higher than that of intein-fused heavy chain transfection alone [(135+/-10) ng/mL vs (37+/-7) ng/mL, Pintein could be used as a technical strategy in a dual-vector system delivering F309SfVIII gene with improved

  5. Determinants of cruise passengers’ expenditures in the port of call

    Directory of Open Access Journals (Sweden)

    Maršenka Marksel

    2016-12-01

    Full Text Available Cruise tourism generates different types of cruise consumption and related indirect, direct and induced expenditure effects, in homeports as well as in ports of call. Cruise passengers’ expenditures produce positive economic effects for destinations, from increasing the incomes and employment, to tax incomes, duties, etc. Therefore, it is no doubt that cruise stakeholders and local economies can benefit from increased cruise passenger consumption. To stimulate higher consumption and passengers’ satisfaction, it is necessary to design the supportive policy framework and build appropriate quality of products and services. Identifying influential variables of cruise passengers’ expenditures in this sense enables the design of appropriate policies and measures. In the current research, based on a survey of 357 cruise passengers, several variables included in a new theoretical model of the expenditures determinants, such as gender, nationality, frequency of cruising and frequency of visits, were found to be statistically significantly associated with cruise passengers’ expenditures. Several conclusions and suggestions to stimulate cruise passenger expenditures based on research findings are provided.

  6. Zwitterionic Group VIII transition metal initiators supported by olefin ligands

    Science.gov (United States)

    Bazan, Guillermo C.; Chen, Yaofeng

    2011-10-25

    A zwitterionic Group VIII transition metal complex containing the simple and relatively small 3-(arylimino)-but-1-en-2-olato ligand that catalyzes the formation of polypropylene and high molecular weight polyethylene. A novel feature of this catalyst is that the active species is stabilized by a chelated olefin adduct. The present invention also provides methods of polymerizing olefin monomers using zwitterionic catalysts, particularly polypropylene and high molecular weight polyethylene.

  7. Worry and its correlates onboard cruise ships.

    Science.gov (United States)

    Wolff, Katharina; Larsen, Svein; Marnburg, Einar; Øgaard, Torvald

    2013-01-01

    The present study examined job-specific worry, as well as possible predictors of such worry, namely job-specific self-efficacy and supervisor dispositionism. 133 non-supervising crew members at different departments onboard upmarket cruise ships filled in a questionnaire during one of their journeys. Findings show that employees report moderate amounts of job-specific worry and the galley crew reports significantly greater amounts of worry than the other departments. Results also indicate that cruise ship crews worry somewhat more than workers in the land based service sector. Furthermore it was found that supervisor dispositionism, i.e. supervisors with fixed mindsets, was related to greater amounts of worry among the crew. Surprisingly, job-specific self-efficacy was unrelated to job-specific worry.

  8. [Hemodialysis on a cruise ship: our logbook].

    Science.gov (United States)

    Valiño Pazos, Cristina; Sáez Rodríguez, Loreto; Meizoso Ameneiro, Ana

    2013-05-01

    After a diet and hydric restriction, travelling is one of the most limiting and worrying activities for patients under chronic renal replacement therapy. The vital dependency on the hemodialysis machine makes travelling an extremely difficult experience. In order to prove the legitimacy of our patients' fears, we embarked on to a cruise-liner with them. This confirmed their limitations when travelling thanks to the reliable data input. This study describes our personal and professional experience as nurses, specialized in hemodialysis, embarking onto a cruise-liner with twelve patients suffering from ESRD. Our goal is to share that experience with professional nurses as well as patients who seek information in regards to this type of trip with a specialized nurse. A summary of this work was presented as a communication oral in the XXXVI Congress of the Spanish Society of Nephrology Nursing.

  9. Collagen-bound von Willebrand factor has reduced affinity for factor VIII.

    Science.gov (United States)

    Bendetowicz, A V; Wise, R J; Gilbert, G E

    1999-04-30

    von Willebrand factor (vWf) is a multimeric adhesive glycoprotein that serves as a carrier for factor VIII in plasma. Although each vWf subunit displays a high affinity binding site for factor VIII in vitro, in plasma, only 2% of the vWf sites for factor VIII are occupied. We investigated whether interaction of plasma proteins with vWf or adhesion of vWf to collagen may alter the affinity or availability of factor VIII-binding sites on vWf. When vWf was immobilized on agarose-linked monoclonal antibody, factor VIII bound to vWf with high affinity, and neither the affinity nor binding site availability was influenced by the presence of 50% plasma. Therefore, plasma proteins do not alter the affinity or availability of factor VIII-binding sites. In contrast, when vWf was immobilized on agarose-linked collagen, its affinity for factor VIII was reduced 4-fold, with KD increasing from 0.9 to 3.8 nM. However, one factor VIII-binding site remained available on each vWf subunit. A comparable reduction in affinity for factor VIII was observed when vWf was a constituent of the subendothelial cell matrix and when it was bound to purified type VI collagen. In parallel with the decreased affinity for factor VIII, collagen-bound vWf displayed a 6-fold lower affinity for monoclonal antibody W5-6A, with an epitope composed of residues 78-96 within the factor VIII-binding motif of vWf. We conclude that collagen induces a conformational change within the factor VIII-binding motif of vWf that lowers the affinity for factor VIII.

  10. MAINTAINING VEHICLE SPEED USING A MECHANICAL CRUISE CONTROL

    Directory of Open Access Journals (Sweden)

    Peter GIROVSKÝ

    2017-06-01

    Full Text Available In this article we would like to present cruise control realization. This cruise control is presented as mechanical device for vehicle speed maintenance and has been proposed as a low cost solution. Principle of function in mechanical cruise control is based on a position control of throttle. For the right action of mechanical cruise control it was need to solve some particular tasks related with speed sensing, construct of device for control of throttle position and design of control system of whole mechanical cruise control. Information about car velocity we have gained using Hall sensor attached on a magnetic ring of car tachometer. For control of the throttle was used a small servo drive and as the control unit was used Arduino. The designed solution of mechanical cruise control have been realized for car Škoda Felicia.

  11. Ehlers-Danlos Syndrome Type VIII: A Rare Cause of Leg Ulcers in Young Patients

    Science.gov (United States)

    Lucas, Antoine; Piérard, Gérald E.; Hermanns-Lê, Trinh; De Paepe, Anne; Dupuy, Alain

    2013-01-01

    Ehlers-Danlos syndrome type VIII (EDS-VIII) is a very rare autosomal dominant disease characterized by early-onset periodontitis associated with features of Ehlers-Danlos syndrome. We report a 32-year-old man whose chronic leg ulcer led to the diagnosis of EDS-VIII. He had severe periodontitis with complete loss of permanent teeth and skin fragility with thin skin, atrophic scars, and brownish atrophic pretibial plaques. Leg ulcer is not a prominent feature of EDS-VIII. We suggest adding EDS-VIII to the list of rare diseases accounting for chronic leg ulcers, if this case report prompts others to report leg ulcers associated with EDS-VIII. PMID:24198978

  12. Economic Impact of Cruise Ship Passengers in Bar Harbor, Maine

    OpenAIRE

    Gabe, Todd; Lynch, Colleen; McConnon, James; Allen, Thomas

    2003-01-01

    This report examines the economic impact of cruise ship passengers in Bar Harbor, Maine. In 2002, 64 cruise ships docked in Bar Harbor carrying about 120,000 passengers and crewmembers. The analysis presented in the report is based on 1,080 passenger surveys conducted between August and October of 2002. Economic impact figures are based on a total of 97,190 passengers, which is the capacity of the 64 cruise ships that were scheduled to visit Bar Harbor in 2002.

  13. 40 CFR Appendix Viii to Part 600 - Fuel Economy Label Formats

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Fuel Economy Label Formats VIII... POLICY FUEL ECONOMY AND CARBON-RELATED EXHAUST EMISSIONS OF MOTOR VEHICLES Pt. 600, App. VIII Appendix VIII to Part 600—Fuel Economy Label Formats EC01MY92.117 EC01MY92.118 EC01MY92.119 EC01MY92.120...

  14. Cruise ship care: a proposed alternative to assisted living facilities.

    Science.gov (United States)

    Lindquist, Lee A; Golub, Robert M

    2004-11-01

    Options for elderly patients who can no longer remain independent are limited. Most choices involve assisted living facilities, 24-hour caregivers, or nursing homes. State and federal assistance for payment for individual care is limited, and seniors usually pay for most costs out of pocket. For those patients who have the means to afford assisted living centers or nursing homes, "cruise ship care" is proposed. Traveling alongside traditional tourists, groups of seniors would live on cruise ships for extended periods of time. Cruise ships are similar to assisted living centers in the amenities provided, costs per month, and many other areas. This article begins with an examination of the needs of seniors in assisted living facilities and then explores the feasibility of cruise ship care in answering those needs. Similarities between cruise ship travel and assisted living care, as well as the monetary costs of both options, are defined. A decision tree with selections for non-independent care for seniors was created including cruise ship care as an alternative. Using a Markov model over 20 years, a representative cost-effectiveness analysis was performed that showed that cruises were priced similarly to assisted living centers and were more efficacious. Proposed ways that cruise ship companies could further accommodate the needs of seniors interested in this option are also suggested. Implementation for cruise ship care on the individual basis is also presented. Ultimately, it is wished to introduce a feasible and possibly more desirable option to seniors who can no longer remain independent.

  15. Customer orientation of cruise destinations in Newfoundland and Labrador, Canada – exploring key issues for ports and the cruise lines

    OpenAIRE

    Hull, John S.; Losekoot, Erwin

    2011-01-01

    This paper evaluates the customer needs of cruise passengers in a context of industry ports in the province of Newfoundland and Labrador, Canada. The study was conducted for the Cruise Association of Newfoundland and Labrador (CANAL) providing primary data as part of their assessment for their Port Readiness Program. The results are generated from a survey of 34 key decision-makers working in 24 ports in the province. Another survey representing the views of 12 cruise lines operating in these...

  16. Cruise Report RV Poseidon cruise POS 295 [POS295], Lisbon 20.03.03 - Las Palmas 01.04.03

    OpenAIRE

    Christiansen, Bernd

    2003-01-01

    The cruise was a pilot cruise in the framework of the EU-project OASIS (Oceanic seamounts: an integrated study) which studies the functioning ecology of seamounts in the NE-Atlantic. The research programme during the cruise included measurements of the physical properties of the water column (temperature and salinity), the sampling of particulate organic matter, measurements of primary production and export fluxes and the sampling of zooplankton.

  17. Cruise Report RV Poseidon cruise POS 295 [POS295], Lisbon 20.03.03 - Las Palmas 01.04.03

    OpenAIRE

    Christiansen, Bernd

    2003-01-01

    The cruise was a pilot cruise in the framework of the EU-project OASIS (Oceanic seamounts: an integrated study) which studies the functioning ecology of seamounts in the NE-Atlantic. The research programme during the cruise included measurements of the physical properties of the water column (temperature and salinity), the sampling of particulate organic matter, measurements of primary production and export fluxes and the sampling of zooplankton.

  18. Improvement of Adaptive Cruise Control Performance

    Directory of Open Access Journals (Sweden)

    Nakagami Takashi

    2010-01-01

    Full Text Available This paper describes the Adaptive Cruise Control system (ACC, a system which reduces the driving burden on the driver. The ACC system primarily supports four driving modes on the road and controls the acceleration and deceleration of the vehicle in order to maintain a set speed or to avoid a crash. This paper proposes more accurate methods of detecting the preceding vehicle by radar while cornering, with consideration for the vehicle sideslip angle, and also of controlling the distance between vehicles. By making full use of the proposed identification logic for preceding vehicles and path estimation logic, an improvement in driving stability was achieved.

  19. Harmonic analysis of a cruise ship network

    Energy Technology Data Exchange (ETDEWEB)

    Guerin, P.; Miegeville, L. [GE44, France (France); Sahnouni, K. [Chantiers de l' Atlantique (France)

    2000-07-01

    The number and the power rating of equipment using power electronics have been increasing on board of the ships for many years. At present, the harmonic disturbance must be taken into account from the design stage in order to ensure the smooth running of the installation. This paper presents a harmonic analysis of the electrical distribution system of a cruise ship. The comparison between the evaluated and the real harmonic levels allows us to discuss about the interest and the limits of the estimates. (authors)

  20. Atypical myocardial infarction on a cruise ship.

    Science.gov (United States)

    Taylor, Christopher

    2015-01-01

    A previously asymptomatic 44-year-old male crewmember on a cruise ship experienced several brief episodes of acute chest pain within a short time frame. He was ultimately diagnosed with myocardial infarction; 5 h earlier he had been discharged from the ship's medical centre after almost 8 h of monitoring to rule-out infarction. Subsequent angiography ashore revealed a 99% occlusion of the right coronary artery. This case highlights the dangers of over-reliance on shipboard cardiac enzyme testing to clear a patient with chest pain.

  1. Factor VIII therapy for hemophilia A: current and future issues.

    Science.gov (United States)

    Aledort, Louis; Ljung, Rolf; Mann, Kenneth; Pipe, Steven

    2014-06-01

    Hemophilia A is a congenital, recessive, X-linked bleeding disorder that is managed with infusions of plasma-derived or recombinant factor (F) VIII. The primary considerations in FVIII replacement therapy today are the: 1) immunogenicity of FVIII concentrates, 2) role of longer-acting FVIII products, 3) prophylactic use of FVIII in children and adults with severe hemophilia A, and 4) affordability and availability of FVIII products. Improving patient outcomes by increasing the use of FVIII prophylaxis, preventing or eliminating FVIII inhibitors, and expanding access to FVIII concentrates in developing countries are the major challenges confronting clinicians who care for patients with hemophilia A.

  2. Integrated Advanced Microwave Sounding Unit-A (AMSU-A. Engineering Report: Electromagnetic Interface (EMI)/Electromagnetic Radiation (EMR) and Electromagnetic Compatibility (EMC), for the METSAT/METOP AMSU-A1

    Science.gov (United States)

    Valdez, A.

    1999-01-01

    This document contains the procedure and the test results of the Advanced Microwave Sounding Unit-A (AMSU-A) Electromagnetic Interference (EMI), Electromagnetic Susceptibility, and Electromagnetic Compatibility (EMC) qualification test for the Meteorological Satellite (METSAT) and the Meteorological Operation Platform (METOP) projects. The test was conducted in accordance with the approved EMI/EMC Test Plan/Procedure, Specification number AE-26151/5D. This document describes the EMI/EMC test performed by Aerojet and it is presented in the following manner: Section-1 contains introductory material and a brief summary of the test results. Section 2 contains more detailed descriptions of the test plan, test procedure, and test results for each type of EMI/EMC test conducted. Section 3 contains supplementary information that includes test data sheets, plots, and calculations collected during the qualification testing.

  3. The C1 and C2 domains of blood coagulation factor VIII mediate its endocytosis by dendritic cells

    Science.gov (United States)

    Gangadharan, Bagirath; Ing, Mathieu; Delignat, Sandrine; Peyron, Ivan; Teyssandier, Maud; Kaveri, Srinivas V.; Lacroix-Desmazes, Sébastien

    2017-01-01

    The development of inhibitory antibodies to therapeutic factor VIII is the major complication of replacement therapy in patients with hemophilia A. The first step in the initiation of the anti-factor VIII immune response is factor VIII interaction with receptor(s) on antigen-presenting cells, followed by endocytosis and presentation to naïve CD4+ T cells. Recent studies indicate a role for the C1 domain in factor VIII uptake. We investigated whether charged residues in the C2 domain participate in immunogenic factor VIII uptake. Co-incubation of factor VIII with BO2C11, a monoclonal C2-specific immunoglobulin G, reduced factor VIII endocytosis by dendritic cells and presentation to CD4+ T cells, and diminished factor VIII immunogenicity in factor VIII-deficient mice. The mutation of basic residues within the BO2C11 epitope of C2 replicated reduced in vitro immunogenic uptake, but failed to prevent factor VIII immunogenicity in mice. BO2C11 prevents factor VIII binding to von Willebrand factor, thus potentially biasing factor VIII immunogenicity by perturbing its half-life. Interestingly, a factor VIIIY1680C mutant, that does not bind von Willebrand factor, demonstrated unaltered endocytosis by dendritic cells as well as immunogenicity in factor VIII-deficient mice. Co-incubation of factor VIIIY1680C with BO2C11, however, resulted in decreased factor VIII immunogenicity in vivo. In addition, a previously described triple C1 mutant showed decreased uptake in vitro, and reduced immunogenicity in vivo, but only in the absence of endogenous von Willebrand factor. Taken together, the results indicate that residues in the C1 and/or C2 domains of factor VIII are implicated in immunogenic factor VIII uptake, at least in vitro. Conversely, in vivo, the binding to endogenous von Willebrand factor masks the reducing effect of mutations in the C domains on factor VIII immunogenicity. PMID:27758819

  4. Dissemination Protocols to Support Cooperative Adaptive Cruise Control (CACC) Merging

    NARCIS (Netherlands)

    Klein Wolterink, W.; Heijenk, Geert; Karagiannis, Georgios

    2011-01-01

    Cooperative adaptive cruise control (CACC) is a form of cruise control in which vehicles cooperatively control their speed using wireless communication. Previously we have implemented CACC using beaconing: the regular broadcasting of status information using 802.11p. Currently we are concerned with

  5. Governing cruise tourism at Bonaire: a networks and flows approach

    NARCIS (Netherlands)

    Bets, van L.K.J.; Lamers, M.A.J.; Tatenhove, van J.P.M.

    2016-01-01

    Conceptual approaches to thoroughly study governance of cruise tourism are lacking in the literature. Relying on Castells’ network society, we analyze how two interconnected flows of cruise ships and passengers are governed by a marine community of users and policy makers. Bonaire is used as a case

  6. Wastewater Pollution from Cruise Ships in the Adriatic Sea

    Directory of Open Access Journals (Sweden)

    Tina Perić

    2016-08-01

    Full Text Available The global growth of cruise tourism has brought increasing concern for the pollution of the marine environment. Marine pollution from sanitary wastewater is a problem especially pronounced on large cruise ships where the number of people on board may exceed 8,000. To evaluate future marine pollution in any selected period of time it is necessary to know the movement of ships in the Adriatic Sea. This paper presents the problem of marine pollution by sanitary wastewater from cruise ships, wastewater treatment technology and a model of cruise ship traffic in the Adriatic Sea considering MARPOL Annex IV areas of limited wastewater discharge. Using the model, it is possible to know in advance the routes of the cruisers and retention time in certain geographic areas. The data obtained by this model can be used as input parameters for evaluation model of wastewater pollution or for evaluation of other types of pollution from cruise ships.

  7. Reduction of Factor VIII Inhibitor Titers During Immune Tolerance Induction With Recombinant Factor VIII-Fc Fusion Protein.

    Science.gov (United States)

    Groomes, Charles L; Gianferante, David M; Crouch, Gary D; Parekh, Dina S; Scott, David W; Lieuw, Kenneth

    2016-05-01

    The development of inhibitors toward factor VIII (FVIII) is a common and serious complication of hemophilia A (HA) therapy. Patients with hemophilia who develop inhibitors often undergo time- and resource-intensive immune tolerance induction (ITI) protocols. We report a 15-month-old male with severe HA and a high-titer inhibitor that occurred while receiving prophylactic treatment with recombinant FVIII (rFVIII), in whom significant inhibitor titer reduction was achieved with thrice weekly infusions of a new, prolonged half-life rFVIII-Fc fusion protein product (trade name Eloctate). Further studies are warranted to explore the potential of Eloctate in ITI protocols.

  8. Cruise missiles should not stop START

    Energy Technology Data Exchange (ETDEWEB)

    Tsipis, K.

    1988-11-01

    A method for verifying a cruise-missile agreement that would be acceptable to the military, industrial, and intelligence communities in both nations must be as unintrusive as possible, while remaining immune to cheating of any significance. This is the goal of the technical solutions outlined here. The elements of a verification regime described do not require routine, intrusive, on-site visits to naval vessels, aircraft, air bases, or weapons magazines where missiles may be stored. They do not interfere with the operational readiness of the missiles, and they protect legitimate military secrets of the inspected nation. If supported by competent national technical means of verification such as those both sides already employ, with a small number of on-site challenge inspections, a combination of technical solutions and procedures such as these could be effective. They would adequately safeguard the national security and sovereignty of the participating nations while providing policymakers with the option of a treaty that limits the number of long-range nuclear cruise missiles or eliminates them completely. As discussed, there are problems that remain to be addressed, but they should not be allowed to block a U.S.-Soviet agreement significantly reducing strategic nuclear arsenals.

  9. A Serotype VIII Strain among Colonizing Group B Streptococcal Isolates in Boston, Massachusetts

    OpenAIRE

    Paoletti, Leanne J.; Bradford, Jessica; Paoletti, Lawrence C.

    1999-01-01

    Maternal colonization with group B Streptococcus (GBS) is a risk factor for neonatal GBS disease. Whereas serotypes Ia, Ib, II, III, and V are prevalent in the United States, types VI and VIII predominate in Japan. Recently, a serotype VIII strain was detected among 114 clinical GBS isolates from a Boston, Mass., hospital.

  10. Effectiveness of Mind Mapping in English Teaching among VIII Standard Students

    Science.gov (United States)

    Hallen, D.; Sangeetha, N.

    2015-01-01

    The aim of the study is to find out the effectiveness of mind mapping technique over conventional method in teaching English at high school level (VIII), in terms of Control and Experimental group. The sample of the study comprised, 60 VIII Standard students in Tiruchendur Taluk. Mind Maps and Achievement Test (Pretest & Posttest) were…

  11. Aerodynamic performances of cruise missile flying above local terrain

    Science.gov (United States)

    Ahmad, A.; Saad, M. R.; Che Idris, A.; Rahman, M. R. A.; Sujipto, S.

    2016-10-01

    Cruise missile can be classified as a smart bomb and also Unmanned Aerial Vehicle (UAV) due to its ability to move and manoeuvre by itself without a pilot. Cruise missile flies in constant velocity in cruising stage. Malaysia is one of the consumers of cruise missiles that are imported from other nations, which can have distinct geographic factors including their local terrains compared to Malaysia. Some of the aerodynamic performances of missile such as drag and lift coefficients can be affected by the local geographic conditions in Malaysia, which is different from the origin nation. Therefore, a detailed study must be done to get aerodynamic performance of cruise missiles that operate in Malaysia. The effect of aerodynamic angles such as angle of attack and side slip can be used to investigate the aerodynamic performances of cruise missile. Hence, subsonic wind tunnel testings were conducted to obtain the aerodynamic performances of the missile at various angle of attack and sideslip angles. Smoke visualization was also performed to visualize the behaviour of flow separation. The optimum angle of attack found was at α=21° and side slip, β=10° for optimum pitching and yawing motion of cruise missile.

  12. Formation of Strategy of Efficient Development of Cruise Tourism

    Directory of Open Access Journals (Sweden)

    Lohunova Natalia A.

    2014-03-01

    Full Text Available The article presents conceptualisation of basic provisions on formation of strategy of efficient development of cruise tourism in Ukraine. Based on the study of problems of the cruise sector development, the article identifies the main goal and structures relevant tasks, justifies main reference points and strategic priorities of development of the cruise industry, specifies principles, mechanisms and expected results of realisation of the designated strategic course. The article states that efficient development of cruise tourism requires creation of an effective system of regulatory and legal, organisational, scientific and methodical, financial and information support. The article offers methodical recommendations on preparation of statistical reports and submission of statistical information about the state and development of the cruise market to the bodies of state statistics, which would allow identification of its quantitative and qualitative parameters, carry out monitoring of the process of creation of the cruise tourist product, analyse practical consequences of measures on stimulation and regulation of the cruise sphere and assess results of realisation of priority directions of strategic development.

  13. Hepatitis E outbreak on cruise ship.

    Science.gov (United States)

    Said, Bengü; Ijaz, Samreen; Kafatos, George; Booth, Linda; Thomas, H Lucy; Walsh, Amanda; Ramsay, Mary; Morgan, Dilys

    2009-11-01

    In 2008, acute hepatitis E infection was confirmed in 4 passengers returning to the United Kingdom after a world cruise. Epidemiologic investigation showed that of 789 persons who provided blood samples, 195 (25%) were seropositive, 33 (4%) had immunoglobulin [Ig] M levels consistent with recent acute infection (11 of these persons were symptomatic), and 162 (21%) had IgG only, consistent with past infection. Passenger mean age was 68 years. Most (426/789, 54%) passengers were female, yet most with acute infection (25/33, 76%) were male. Sequencing of RNA from 3 case-patients identified hepatitis E virus genotype 3, closely homologous to genotype 3 viruses from Europe. Significant association with acute infection was found for being male, drinking alcohol, and consuming shellfish while on board (odds ratio 4.27, 95% confidence interval 1.23-26.94, p = 0.019). This was probably a common-source foodborne outbreak.

  14. 75 FR 82243 - Security Zones; Moored Cruise Ships, Port of San Diego, CA

    Science.gov (United States)

    2010-12-30

    ... a 100 yard radius around any cruise ship that is moored at any berth within the San Diego port area... for the safety of the cruise ship, vessels, and users of the waterway. Entry into these security zones... Broadway cruise ship terminal and the anticipated arrival of cruise ships immediately thereafter,...

  15. Acquired factor VIII inhibitor syndrome: A rare cause of hematuria

    Directory of Open Access Journals (Sweden)

    Muthuvel Seral Kannan

    2015-01-01

    Full Text Available A 50-year-old woman presented with gross hematuria for 1 month. Clinical examinations, laboratory investigations, ultrasound and contrast computed tomography were normal, except anemia. Cystoscopy revealed bloody efflux from the right side. Retrograde pyelogram showed filling defect in the renal pelvis and biopsy was inconclusive. Renal angiogram was normal. She developed ecchymosis on the right thigh and arm with elevated activated partial thromboplastin time. The partial thromboplastin time correction study and Bethesda study confirmed the presence of acquired factor VIII inhibitor (acquired hemophilia. With flexible ureterorenoscopy, the mass in the renal pelvis was removed and its histopathology revealed clotted blood. The patient was subsequently managed with steroids and Factor eight inhibitor bypass activity.

  16. Evaluation of Factor VIII as a Risk Factor in Indian Patients with DVT.

    Science.gov (United States)

    Hazra, Darpanarayan; Sen, Indrani; Stephen, Edwin; Agarwal, Sunil; Nair, Sukesh Chandran; Mammen, Joy

    2015-01-01

    Introduction. Elevated factor VIII population in the Indian population has not been studied as a possible risk factor for deep vein thrombosis (DVT). High factor VIII level is considered a predisposing factor for DVT and its recurrence. However it is known to vary between populations and its exact role in the etiopathogenesis of thrombophilia remains unknown. Material and Methods. Factor VIII levels of patients with DVT who had undergone a prothrombotic workup as a part of their workup was compared to normal age matched controls in a 1 : 3 ratio. Results. There were 75 patients with DVT who had undergone a prothrombotic workup in the course of their treatment for lower limb DVT. In these, 64% had levels of factor VIII more than 150 as compared to 63% of normal controls (p > 0.05, not significant). Conclusion. Elevated factor VIII in the Indians may not be associated with the same thrombotic risk as seen in the West. We find a variation in the levels of factor VIII with a different "normal" than what is reported in other populations. This needs further study to elucidate the role of factor VIII in the evaluation and treatment of thrombophilia.

  17. Pining for home: Studying crew homesickness aboard a cruise liner

    African Journals Online (AJOL)

    Research in Hospitality Management is co-published by NISC (Pty) Ltd and Routledge, Taylor & Francis Group ... homesickness as a longing for the people, places and ..... has to be recognised as a serious issue for the cruise industry,.

  18. Passive Acoustic Radar for Detecting Supersonic Cruise Missile

    Institute of Scientific and Technical Information of China (English)

    XIAO Feng; XIAO Hui

    2005-01-01

    A Passive Acoustic Radar is presented as a necessary complement to electromagnetic wave radar, which will be expected to be an effective means for detecting cruise missiles. Acoustic characteristics of supersonic flying projectiles with diverse shapes are expounded via experiment. It is pointed out that simulation experiment could be implemented using bullet or shell instead of cruise missile. Based on theoretical analysis and experiment, the "acoustic fingerprint" character of cruise missile is illustrated to identify it in a strong noise environment. After establishing a locating mathematical model,the technique of acoustic embattling is utilized to resolve a problem of confirming the time of early-warning, considering the fact that velocity of sound is much slower than that of light. Thereby, a whole system of passive acoustic radar for detecting supersonic cruise missile is formed.

  19. Storyboard GALILEO CRUISE SCIENCE OPPORTUNITIES describes asteroid encounters

    Science.gov (United States)

    1989-01-01

    Storyboard with mosaicked image of an asteroid and entitled GALILEO CRUISE SCIENCE OPPORTUNITIES describes asteroid objectives. These objectives include: first asteroid encounter; surface geology, composition size, shape, mass; and relation of primitive bodies to meteorites.

  20. Modeling the seakeeping performance of luxury cruise ships

    Science.gov (United States)

    Cao, Yu; Yu, Bao-Jun; Wang, Jian-Fang

    2010-09-01

    The seakeeping performance of a luxury cruise ship was evaluated during the concept design phase. By comparing numerical predictions based on 3-D linear potential flow theory in the frequency domain with the results of model tests, it was shown that the 3-D method predicted the seakeeping performance of the luxury cruise ship well. Based on the model, the seakeeping features of the luxury cruise ship were analyzed, and then the influence was seen of changes to the primary design parameters (center of gravity, inertial radius, etc.). Based on the results, suggestions were proposed to improve the choice of parameters for luxury cruise ships during the concept design phase. They should improve seakeeping performance.

  1. InRidge program: Preliminary results from the first cruise

    Digital Repository Service at National Institute of Oceanography (India)

    Mukhopadhyay, R.; Murthy, K.S.R.; Iyer, S.D.; Rao, M.M.M.; Banerjee, R.; Subrahmanyam, A.S.; Shirodkar, P.V.; Ghose, I.

    The first cruise under India's own Ridge research initiative, InRidge collected new data on bathymetry, free-air gravity and magnetic anomalies across the ridge axis between the Vema and Zhivago transform faults in the Central Indian Ridge...

  2. An outbreak of Cyclospora infection on a cruise ship.

    Science.gov (United States)

    Gibbs, R A; Nanyonjo, R; Pingault, N M; Combs, B G; Mazzucchelli, T; Armstrong, P; Tarling, G; Dowse, G K

    2013-03-01

    In 2010, an outbreak of cyclosporiasis affected passengers and crew on two successive voyages of a cruise ship that departed from and returned to Fremantle, Australia. There were 73 laboratory-confirmed and 241 suspected cases of Cyclospora infection reported in passengers and crew from the combined cruises. A case-control study performed in crew members found that illness was associated with eating items of fresh produce served onboard the ship, but the study was unable conclusively to identify the responsible food(s). It is likely that one or more of the fresh produce items taken onboard at a south-east Asian port during the first cruise was contaminated. If fresh produce supplied to cruise ships is sourced from countries or regions where Cyclospora is endemic, robust standards of food production and hygiene should be applied to the supply chain.

  3. Efficacy and safety of rVIII-Singlechain : Results of a phase 1/3 multicenter clinical trial in severe hemophilia A

    NARCIS (Netherlands)

    Mahlangu, Johnny; Kuliczkowski, Kazimierz; Karim, Faraizah Abdul; Stasyshyn, Oleksandra; Kosinova, Marina V.; Lepatan, Lynda Mae; Skotnicki, Aleksander; Boggio, Lisa N.; Klamroth, Robert; Oldenburg, Johannes; Hellmann, Andrzej; Santagostino, Elena; Baker, Ross I.; Fischer, Kathelijn; Gill, Joan C.; P'Ng, Stephanie; Chowdary, Pratima; Escobar, Miguel A.; Khayat, Claudia Djambas; Rusen, Luminita; Bensen-Kennedy, Debra; Blackman, Nicole; Limsakun, Tharin; Veldman, Alex; Ledger, Katie St; Pabinger, Ingrid

    2016-01-01

    Recombinant VIII (rVIII)-SingleChain is a novel B-domain–truncated recombinant factor VIII (rFVIII), comprised of covalently bonded factor VIII (FVIII) heavy and light chains. It was designed to have a higher binding affinity for von Willebrand factor (VWF). This phase 1/3 study investigated the eff

  4. European River Cruises On the Rise Among American Tourists

    Directory of Open Access Journals (Sweden)

    Melinda Jászberényi, Ph.D.

    2014-10-01

    Full Text Available River cruising is one of the most attractive and rapidly developing areas of international tourism. Beyond the beautiful natural environment of the rivers, architectural attractions along the riverside enrich the experience, providing historical and cultural background that deepens tourists’ connections to the city. This article provides an overview of Danube river cruise tourism among American tourist experts. It also showcases Budapest, an increasingly important and internationally recognized port of the Danube and capital of Hungary, as a popular tourist destination.

  5. Cruise ship's doctors - company employees or independent contractors?

    Science.gov (United States)

    Dahl, Eilif

    2016-01-01

    Traditionally, cruise companies have stated that they are in the transport business but not in the business of providing medical services to passengers. They have claimed not to be able to supervise or control the ship's medical personnel and cruise ship's doctors have therefore mostly been signed on as independent contractors, not employees. A United States court decision from 1988, Barbetta versus S/S Bermuda Star, supported this view and ruled that a ship's owner cannot be held vicariously liable for the negligence of the ship's doctor directed at the ship's passengers. Some years ago a cruise passenger fell and hit his head while boarding a trolley ashore. Hours later he was seen aboard by the ship's doctor, who sent him to a local hospital. He died 1 week later, and his daughter filed a complaint alleging the cruise company was vicariously liable for the purported negligence of the ship's doctor and nurse, under actual or apparent agency theories. A United States district court initially dismissed the case, but in November 2014 the United States Court of Appeals for the Eleventh Circuit disagreed and reversed. From then on independently contracted ship's doctors may be considered de facto employees of the cruise line. The author discusses the employment status of physicians working on cruise ships and reviews arguments for and against the Appellate Court's decision.

  6. Learning taxis’ cruising patterns with Ripley’s K function

    Institute of Scientific and Technical Information of China (English)

    宗芳; 张慧永

    2015-01-01

    Taxi drivers’ cruising patterns are learnt with GPS trajectory data collected in Shenzhen, China. By employing Ripley’s K function, the impacts of land use and pick-up experience on taxis’ cruising behavior are investigated concerning about both intensity of influence and radius of influence. The results indicate that, in general, taxi drivers tend to learn more from land use characteristics than from pick-up experience. The optimal radius of influence of land use points and previous pick-up points is 14.18 km and 9.93 km, respectively. The findings also show that the high-earning drivers or thorough drivers pay more attention to land use characteristics and tend to cruise in high-density area, while the low-earning drivers or focus drivers prefer to learn more from previous pick-up experience and select the area which is far away from the high-density area. These findings facilitate the development of measures of managing taxi’s travel behavior by providing useful insights into taxis’ cruising patterns. The results also provide useful advice for taxi drivers to make efficient cruising decision, which will contribute to the improvement of cruising efficiency and the reduction of negative effects.

  7. Cruise medicine: the dental perspective on health care for passengers during a world cruise.

    Science.gov (United States)

    Sobotta, Bernhard A J; John, Mike T; Nitschke, Ina

    2008-01-01

    Although more than 100 million passengers have taken a cruise since 1980, it is not known what dental treatment needs occur at sea. The routine dental documentation of a 2-month period at sea on a cruise ship carrying 1,619 passengers was analyzed. The subjects for the study were 57 passengers (3.5% of 1,619), with a mean age of 71 years (+/-9.8 y). Age, gender, number of natural teeth and implants, prosthetic status, diagnosis, treatment performed, percentage of emergency and routine procedures, number of appointments, duration of appointment, time since last visit to the dentist, and cabin category as indicator of socioeconomic status were extracted. Oral health-related quality of life (OHRQoL) was measured using the 14-item Oral Health Impact Profile. Passengers had a mean number of 20 natural teeth plus substantial fixed and removable prosthodontics. Emergency dental treatment accounted for 97% of the chairside time. The three most frequent emergency diagnoses were defective restorations (36%), pulpal disease (20%), and defective prosthesis and caries (both 11.5%). Common emergency therapies provided were complex surgical-prosthodontic rehabilitation, various endodontic treatments, and extractions. Per 1,000 persons/month, passengers required 21.6 emergency plus 2.5 routine appointments; 49% of passengers had seen a dentist within 3 months before going to sea. Passengers do attend their dentist for routine care/checkups before the voyage, yet experience complex dental emergencies. This is due to the presence of a high number of restorations that fail unexpectedly. Some failures are so severe that they would have forced the passenger to abort the cruise had there been no dental service available. The ease of access to quality dental care may explain the relatively low level of perceived problems as characterized by OHRQoL scores.

  8. Case of acquired hemophilia with factor VIII inhibitor in a mother and newborn.

    Science.gov (United States)

    Kotani, Yasushi; Shiota, Mitsuru; Umemoto, Masahiko; Koike, Eiji; Tsuritani, Mitsuhiro; Hoshiai, Hiroshi

    2011-08-01

    We report a mother and newborn in the puerperium with hemorrhage secondary to factor VIII inhibitor. A 31-year-old gravida 1 para 1 delivered at a local clinic with a massive postpartum hemorrhage. The activated partial thromboplastin time was prolonged and factor VIII inhibitor was detected. The persistent hemorrhage improved following treatment, including transfusion, steroid therapy, and bypass therapy with factor VII formulations. After hysteroscopic removal of the retained placenta, the hemorrhage decreased. The newborn developed significant swelling of the hands after routine blood sampling and factor VIII inhibitor was detected. The inhibitor disappeared without any special treatment in the 5th month postpartum in the mother and the 4th month postpartum in the newborn. Factor VIII inhibitor may be transferred via the placenta from the mother to the fetus. Therefore, the newborn should also be carefully observed in a case of massive hemorrhage after delivery.

  9. A case of renal infarction associated with elevated factor VIII level.

    Science.gov (United States)

    Siddiqui, O Asif; Al-Absi, A; Showkat, A

    2011-09-01

    Elevated factor VIII level has recently been shown to be associated with increased risk of thrombosis. We report here a case of renal infarction in association with elevated factor VIII level. The patient presented with a three-day history of flank pain. Laboratory studies on presentation showed an elevated serum creatinine concentration and microscopic hematuria. He was found to have bilateral pulmonary emboli and left common femoral vein thrombosis; imaging studies showed evidence of renal arterial thrombosis with infarction. Hypercoagulability assessment showed an elevated factor VIII level. He was treated with heparin and warfarin with significant improvement in his renal function. Consideration should be given to measurement of factor VIII level as a part of the workup of unexplained thrombo-embolic events.

  10. Solar Wind Charge Exchange and O VIII emission toward MBM 12

    Science.gov (United States)

    Edgar, Richard J.; Wargelin, B. J.; Raymond, J. C.; Slavin, J. D.; Smith, R. K.; Kharchenko, V.

    2006-09-01

    A Chandra observation toward MBM-12, a nearby (MBM 12 during the Chandra observation. The Solar Wind Charge Exchange mechanism can account for essentially all of the O VIII emission seen in this observation.

  11. Extrahepatic sources of factor VIII potentially contribute to the coagulation cascade correcting the bleeding phenotype of mice with hemophilia A.

    Science.gov (United States)

    Zanolini, Diego; Merlin, Simone; Feola, Maria; Ranaldo, Gabriella; Amoruso, Angela; Gaidano, Gianluca; Zaffaroni, Mauro; Ferrero, Alessandro; Brunelleschi, Sandra; Valente, Guido; Gupta, Sanjeev; Prat, Maria; Follenzi, Antonia

    2015-07-01

    A large fraction of factor VIII in blood originates from liver sinusoidal endothelial cells although extrahepatic sources also contribute to plasma factor VIII levels. Identification of cell-types other than endothelial cells with the capacity to synthesize and release factor VIII will be helpful for therapeutic approaches in hemophilia A. Recent cell therapy and bone marrow transplantation studies indicated that Küpffer cells, monocytes and mesenchymal stromal cells could synthesize factor VIII in sufficient amount to ameliorate the bleeding phenotype in hemophilic mice. To further establish the role of blood cells in expressing factor VIII, we studied various types of mouse and human hematopoietic cells. We identified factor VIII in cells isolated from peripheral and cord blood, as well as bone marrow. Co-staining for cell type-specific markers verified that factor VIII was expressed in monocytes, macrophages and megakaryocytes. We additionally verified that factor VIII was expressed in liver sinusoidal endothelial cells and endothelial cells elsewhere, e.g., in the spleen, lungs and kidneys. Factor VIII was well expressed in sinusoidal endothelial cells and Küpffer cells isolated from human liver, whereas by comparison isolated human hepatocytes expressed factor VIII at very low levels. After transplantation of CD34(+) human cord blood cells into NOD/SCIDγNull-hemophilia A mice, fluorescence activated cell sorting of peripheral blood showed >40% donor cells engrafted in the majority of mice. In these animals, plasma factor VIII activity 12 weeks after cell transplantation was up to 5% and nine of 12 mice survived after a tail clip-assay. In conclusion, hematopoietic cells, in addition to endothelial cells, express and secrete factor VIII: this information should offer further opportunities for understanding mechanisms of factor VIII synthesis and replenishment.

  12. Purification of coagulation factor VIII by immobilized metal affinity chromatography.

    Science.gov (United States)

    Rodrigues, Estela S; Verinaud, Claudia I; Oliveira, Douglas S; Raw, Isaías; Lopes, Alexandre P Y; Martins, Elizabeth A L; Cheng, Elisabeth

    2015-01-01

    Factor VIII (FVIII) is a glycoprotein that plays an essential role in blood coagulation cascade. Purification of plasma-derived coagulation FVIII by direct application of plasma to a chromatographic column is a method of choice. Anion exchange column is a very powerful method because FVIII is strongly adsorbed, resulting in good activity recovery and high purification factor. However, vitamin-K-dependent coagulation factors coelute with FVIII. In the present study, we report the separation of vitamin-K-dependent coagulation proteins from FVIII using immobilized metal affinity chromatography (IMAC) with Cu(2+) as the metal ligand. Plasma was directly loaded to a Q Sepharose Big Beads column, and FVIII was recovered with 65% activity and a purification factor of approximately 50 times. Then, the Q Sepharose eluate was applied to the IMAC-Cu(2+) column, and FVIII was eluted with 200 mM imidazole, with up to 85% recovery of activity. The mass recovery in this fraction was less than 10% of the applied mass of protein. Vitamin-K-dependent proteins elute with imidazole concentrations of lower than 60 mM. Because of the difference in affinity, FVIII could be completely separated from the vitamin-K-dependent proteins in the IMAC column.

  13. piggyBac-mediated phenotypic correction of factor VIII deficiency

    Directory of Open Access Journals (Sweden)

    Janice M Staber

    2014-01-01

    Full Text Available Hemophilia A, caused by a deficiency in factor VIII (FVIII, is the most severe inherited bleeding disorder. Hemophilia A is an attractive gene therapy candidate because even small increases in FVIII levels will positively alter the phenotype. While several vectors are under investigation, gene addition from an integrated transgene offers the possibility of long term expression. We engineered the DNA transposon-based vector, piggyBac (PB, to carry a codon-optimized B-domain deleted human FVIII cDNA. Evaluation of gene transfer efficiency in FVIII null mice demonstrated that PB containing the FVIII cDNA, delivered via hydrodynamic injection to immunocompetent hemophilia mice, conferred persistent gene expression, attaining mean FVIII activity of approximately 60% with 3/19 developing inhibitors. In addition to efficacious expression, a goal of gene transfer-based therapies is to develop vectors with low toxicity. To assess endoplasmic reticulum stress in hepatocytes stably expressing the transgene, we evaluated levels of ER stress markers via qPCR and found no evidence of cell stress. To evaluate phenotypic correction, a tail clip assay performed at the end of the study revealed reduced blood loss. These data demonstrate that PB can be used to achieve sustained FVIII expression and long-term therapeutic benefit in a mouse model.

  14. Development and characterization of lipidic cochleate containing recombinant factor VIII

    Science.gov (United States)

    Miclea, Razvan D.; Varma, Prashant R.; Peng, Aaron; Balu-Iyer, Sathy V.

    2007-01-01

    Hemophilia A, a life threatening bleeding disorder is caused by deficiency of Factor VIII (FVIII). Replacement therapy using rFVIII is the first line therapy for hemophilia A. However, 15-30% of patients develop neutralizing antibody, mainly against the C2, A3 and A2 domains. It has been reported that PS-FVIII complex reduced total and neutralizing anti-rFVIII antibody titers in hemophilia A murine models. Here, we developed FVIII – containing cochleate cylinders, utilizing PS-Ca2+ interactions and characterized these particles for optimal in vivo properties using biophysical and biochemical techniques. Approximately 75% of the protein was associated with cochleate cylinders. Sandwich ELISA, acrylamide quenching and enzymatic digestion studies established that rFVIII was shielded from the bulk aqueous phase by the lipidic structures, possibly leading to improved in vivo stability. Freeze – thawing and rate limiting diffusion studies revealed that small cochleate cylinders with a particles size of 500 nm or less could be generated. The release kinetics and in vivo experiments suggested that there is slow and sustained release of FVIII from the complex upon systemic exposure. In vivo studies using tail clip method indicated that FVIII-cochleate complex is effective and protects hemophilic mice from bleeding. Based on these studies, we speculate that the molecular interaction between FVIII and PS may provide a basis for the design of novel FVIII lipidic structures for delivery applications. PMID:17936245

  15. A study of reported factor VIII use around the world.

    Science.gov (United States)

    Stonebraker, J S; Brooker, M; Amand, R E; Farrugia, A; Srivastava, A

    2010-01-01

    The effect of replacement therapy has significantly improved the morbidity and mortality of people with haemophilia A in high income countries, a recent socio-economic development as the availability of safe concentrates has been matched by a willingness for their provision through reimbursement. In the developing world, however, this state has not been achieved, primarily because of the low visibility of haemophilia coupled with its expense, leading to inadequate treatment with its sequelae of severe pain, joint deformities, arthropathy, disabilities, and even death in childhood or early adult life. The objective of this paper was to study the reported factor VIII (FVIII) use on a country-by-country basis. Data on the reported FVIII use for 104 countries were obtained from the Marketing Research Bureau, Inc. and the World Federation of Hemophilia. The results show that FVIII use varies considerably among countries, even among the wealthiest of countries. The use of FVIII concentrate increases as economic capacity increases; in addition, consumption of FVIII has been increasing at a greater rate in high income countries. Given these trends, there probably will be a global increase in FVIII concentrates usage. Such information is critical for national healthcare agencies to determine realistic budget priorities in planning for an increased allocation of resources required to improve the treatment of patients with haemophilia A. This information is also important for pharmaceutical manufacturers to adequately plan for increased production of FVIII concentrates.

  16. Integrated Advanced Microwave Sounding Unit-A (AMSU-A). Test Report, Electromagnetic Interference (EMI)/Electromagnetic Radiation(EMR) and Electromagnetic Capability (EMC) for the EOS/AMSU-A1

    Science.gov (United States)

    Paliwoda, L.

    1998-01-01

    This document contains the procedure and the test results of the Advanced Microwave Sounding Unit-A (AMSU-A) Earth Observing System (EOS) Project, assembly part number 1356008-1, serial number 202, Electromagnetic Interference (EMI) and Electromagnetic Susceptibility (EMC) qualification test. The test was conducted in accordance with the approved EMI/EMC Test Plan/Procedure, Specification number AE-26151/8B, dated 10 September 1998. Aerojet intends that the presentation and submittal of this document, prepared in accordance with the objectives established by the aforementioned Test Plan/Procedure, document number AE-26151/8B, will satisfy the data requirement with respect to the AMSU-A/EOS instrument operational compliance of the EMI/EMC test requirement. Test for the AMSU-A/EOS instrument have been completed and all the requirements per General Interface Requirement Document (GIRD), GSFC 422-11-12-01, for EOS Common Spacecraft/Instruments, paragraph 10.11, were met with the exceptions of the test methods CE03, RE01, and RE02, as described in this document.

  17. BALINESE WOMEN IN THE CRUISE SHIPS TOURISM INDUSTRY

    Directory of Open Access Journals (Sweden)

    I Made Darma Oka

    2016-03-01

    Full Text Available As one of the most popular destinations for international tourists, Bali has attracted a sizeable and growing labor force in the tourism sector of the economy. This fact has triggered Balinese labor force to participate in such service industry. As a supplier of tourism labor force Bali has been increasingly successful in promoting the number of workers to be employed on cruise ships. The participation rate of Balinese women in cruise industry over the last four years has dramatically increased. The Balinese women’s participation in cruise ship employment has brought major implications for their life and culture. Generally, the present study aimed to provide an overview of Balinese women employed on board of a cruise ship. More specifically, it examined (1 the strengths, weaknesses, opportunities, and threats faced by Balinese women working in cruise industry, (2 factors influencing them to work in the industry, and (3 the implications brought by such employment for their life and society. The present study used quantitative and qualitative data collected through economic, social, and cultural approach. The sample was comprised of 200 respondents selected using accidental sampling method. To answer the research questions, data collection was conducted through observation, interviews, as well as focus group discussion (FGD. The data on the strengths, weaknesses, opportunities, and threats faced by Balinese women on board of a cruise ship were analyzed using SWOT analysis, whereas the data on the factors influencing them to work in the cruise industry were analyzed using factor analysis. Finally, qualitative analysis was employed to analyze the data on the economic, social, and cultural implications for their life. The analysis showed that: (1 Balinese women were employed on board of a cruise linerpredominantly as support staff. The strengths of Balinese women cruise ship workers included being friendly, always smiling, being honest, being loyal

  18. Participation in the 1996 Arlindo Cruise to the Indonesian Seas

    Science.gov (United States)

    Marra, John

    1997-01-01

    The objective of Arlindo-Productivity is to understand the factors responsible for regional differences in the response of phytoplankton and zooplankton to the SE and NW Monsoons in Indonesia. The hypothesis is that an interplay between circulation and shoaling of the nutricline, as a response to the monsoons, regulates productivity in the Indonesian Seas. My o@jective for the cruise in 1996 was to continue our collaboration with Indonesian scientists by conducting a set of hydrographic, primary production and spectral irradiance observations in the Indonesian Seas. This grant paid for shipping, travel and incidental costs associated with participation in the cruise in December, 1996. Ship costs were borne by the Indonesian Institute of Sciences as part of the collaborative effort. A plan for Arlindo in 1996 was agreed upon in March, 1996, by Indonesian scientists together with Arnold Gordon. The plan called for a 20-day physical oceanography and mooring cruise in November, 1996, followed by a 5-day bio-optical cruise. The bio-optical cruise departed from, and returned to, Ambon, and sampled in the Banda Sea. We completed a series of chlorophyll analyses, both a sampling of surface variability and depth profiles in the Banda Sea. We also completed three MER profiles for depth profiles of spectral irradiance. These data have a useful by-product in that they can be used for vicarious calibration of the OCTS sensor aboard the ADEOS satellite. As such, the data has been transmitted to NASDA in Japan for their use.

  19. Impressions of Serbia: Tourists on cruises along Corridor 7

    Directory of Open Access Journals (Sweden)

    Dragin Aleksandra S.

    2009-01-01

    Full Text Available This paper deals with cruises along Corridor 7 and the tourist offer of Serbia. The purpose of the paper has been to establish how international tourists see our country during their travels across Serbia on cruises along Corridor 7. The research has been based on the interviews with international tourists who participated in land tours while cruising through Serbia. The interviews, together with the structured questionnaire, were conducted from March to November 2007 with the objectives to establish the following: the structure of the respondents according to the country of origin, gender and age structure, as well as the social and economic structure; what their motives are for cruising along Corridor 7; what is their perception of the value obtained through the tourist product and services during the Corridor 7 cruises and during their stay in Serbia (what they liked best in Serbia; if they were dissatisfied with anything in terms of the tourist offer of Serbia; to what extent their visit complemented or influenced their personal impressions of this country - what their impressions were before and after the visit. The importance of this paper, above all, is in broadening our knowledge about the adequacy of the tourist offer in Serbia in the tourism segment which is the subject of study of the paper.

  20. Effectiveness of Basic Safety Training among Cruise Line Students

    Directory of Open Access Journals (Sweden)

    Dr. Dexter R. Buted

    2014-06-01

    Full Text Available – This study aimed to assess the basic safety training among cruise line students in Lyceum of the Philippines University. Specifically, it sought to identify the effectiveness of basic safety training in terms of reaction, behaviour, learning and results; the problems encountered by the respondents’ during their basic safety training; and lastly, propose a program to enhance the BST program among cruise line operation students. The study made use of descriptive method and using the Kirkpatrick Evaluation Training Model. The respondents agreed that the basic safety training programs for cruise line operations students are effective since it enhances their learning and behaviour. The results and reaction coming from the students who underwent basic safety training implies that they are trained and helped by the program. They sometimes experienced problems during their training. However students believed that the institution were proficient in providing programs and have already foreseen the problems that might occur and provided contingency plan for each. A program is proposed to enhance the basic safety training of LPUBatangas. Continuous training may be done for reinforcement of each students and level of organization so that the cruise line students will be more competitive and be more productive. Strict compliance of the cruise line students to the training program may be implemented.

  1. von Willebrand factor binds to the surface of dendritic cells and modulates peptide presentation of factor VIII.

    Science.gov (United States)

    Sorvillo, Nicoletta; Hartholt, Robin B; Bloem, Esther; Sedek, Magdalena; ten Brinke, Anja; van der Zwaan, Carmen; van Alphen, Floris P; Meijer, Alexander B; Voorberg, Jan

    2016-03-01

    It has been proposed that von Willebrand factor might affect factor VIII immunogenicity by reducing factor VIII uptake by antigen presenting cells. Here we investigate the interaction of recombinant von Willebrand factor with immature monocyte-derived dendritic cells using flow cytometry and confocal microscopy. Surprisingly, von Willebrand factor was not internalized by immature dendritic cells, but remained bound to the cell surface. As von Willebrand factor reduces the uptake of factor VIII, we investigated the repertoire of factor VIII presented peptides when in complex with von Willebrand factor. Interestingly, factor VIII-derived peptides were still abundantly presented on major histocompatibility complex class II molecules, even though a reduction of factor VIII uptake by immature dendritic cells was observed. Inspection of peptide profiles from 5 different donors showed that different core factor VIII peptide sequences were presented upon incubation with factor VIII/von Willebrand factor complex when compared to factor VIII alone. No von Willebrand factor peptides were detected when immature dendritic cells were pulsed with different concentrations of von Willebrand factor, confirming lack of von Willebrand factor endocytosis. Several von Willebrand factor derived peptides were recovered when cells were pulsed with von Willebrand factor/factor VIII complex, suggesting that factor VIII promotes endocytosis of small amounts of von Willebrand factor by immature dendritic cells. Taken together, our results establish that von Willebrand factor is poorly internalized by immature dendritic cells. We also show that von Willebrand factor modulates the internalization and presentation of factor VIII-derived peptides on major histocompatibility complex class II.

  2. Implications of an updated ultraviolet background for the ionization mechanisms of intervening Ne VIII absorbers

    Science.gov (United States)

    Hussain, Tanvir; Khaire, Vikram; Srianand, Raghunathan; Muzahid, Sowgat; Pathak, Amit

    2017-04-01

    Ne VIII absorbers seen in QSO spectra are useful tracers of warm ionized gas, when collisional ionization is the dominant ionization process. While photoionization by the ultraviolet background (UVB) is a viable option, it tends to predict large line-of-sight thickness for the absorbing gas. Here, we study the implications of the recently updated UVB at low z to understand the ionization mechanisms of intervening Ne VIII absorbers. With the updated UVB, one typically needs higher density and metallicity to reproduce the observed ionic column densities under photoionization. Both reduce the inferred line-of-sight thicknesses of the absorbers. We find a critical density of ≥5 × 10-5 cm-3 above which the observed N({Ne VIII})/N({O VI}) can be reproduced by pure collisional processes. If the gas is of near solar metallicity (as measured for the low ions) then the cooling time-scales will be small (<108 yrs). Therefore, a continuous injection of heat is required in order to enhance the detectability of the collisionally ionized gas. Using photoionization models we find that in almost all Ne VIII systems the inferred low ion metallicity is near solar or supersolar. If we assume the Ne VIII phase to have similar metallicities then photoionization can reproduce the observed N({Ne VIII})/N({O VI}) without the line-of-sight thickness being unreasonably large and avoids cooling issues related to the collisional ionization at these metallicities. However, the indication of broad Lyα absorption in a couple of systems, if true, suggests that the Ne VIII phase is distinct from the low ion phase having much lower metallicity.

  3. Potentiation of thrombin generation in hemophilia A plasma by coagulation factor VIII and characterization of antibody-specific inhibition.

    Directory of Open Access Journals (Sweden)

    Bhavya S Doshi

    Full Text Available Development of inhibitory antibodies to coagulation factor VIII (fVIII is the primary obstacle to the treatment of hemophilia A in the developed world. This adverse reaction occurs in 20-30% of persons with severe hemophilia A treated with fVIII-replacement products and is characterized by the development of a humoral and neutralizing immune response to fVIII. Patients with inhibitory anti-fVIII antibodies are treated with bypassing agents including recombinant factor VIIa (rfVIIa. However, some patients display poor hemostatic response to bypass therapy and improved treatment options are needed. Recently, we demonstrated that fVIII inhibitors display widely variable kinetics of inhibition that correlate with their respective target epitopes. Thus, it was hypothesized that for antibodies that display slow rates of inhibition, supplementation of rfVIIa with fVIII would result in improved thrombin generation and be predictive of clinical responses to this novel treatment regimen. In order to test this hypothesis, 10 murine monoclonal antibodies (MAbs with non-overlapping epitopes spanning fVIII, differential inhibition titers, and inhibition kinetics were studied using a thrombin generation assay. Of the 3 MAbs with high inhibitory titers, only the one with fast and complete (classically defined as "type I" kinetics displayed significant inhibition of thrombin generation with no improvement upon supplementation of rfVIIa with fVIII. The other two MAbs that displayed incomplete (classically defined as "type II" inhibition did not suppress the potentiation of thrombin generation by fVIII. All antibodies that did not completely inhibit fVIII activity demonstrated potentiation of thrombin generation by the addition of fVIII as compared to rfVIIa alone. In conclusion, fVIII alone or in combination with rfVIIa corrects the thrombin generation defect produced by the majority of anti-fVIII MAbs better than single agent rfVIIa. Therefore, combined fVIII

  4. Methods of Raising Funds for Purchasing of New Cruise Ships by International Corporations

    Directory of Open Access Journals (Sweden)

    Kizielewicz Joanna

    2017-06-01

    Full Text Available The world’s cruise corporations regularly purchase large, luxurious cruise ships. In accordance with the Cruise Line International Association, 33 new ocean cruise ships will be available on the market by 2020. These types of capital expenditures are associated with large financial outlays of up to $ 1 billion. The leading cruise corporations are not able to finance purchases of new units with their own resources and therefore look for different solutions. Available publications focus mainly on issues related to purchasing cargo ships, not cruise ships. The objective of the article is to identify sources of funding of new cruise ships. Our analysis identifies the average capital expenditure associated with purchasing new cruise ships and factors that influence it. The most popular methods for raising such capital are also provided. Our research methodology relies on data exploration method, a desk research method and comparative analysis.

  5. FRV Deleware II cruise, 30 June to 7 July 1978. Data report

    Energy Technology Data Exchange (ETDEWEB)

    Behrens, W.; von Bock, K. (eds.)

    1982-05-01

    This was the last of three companion cruises designed to provide broad-scale coverage of seasonal shelf conditions occurring between the April and October investigations undertaken aboard ATLANTIS II cruises 99 and 104.

  6. Acute Gastroenteritis on Cruise Ships - United States, 2008-2014.

    Science.gov (United States)

    Freeland, Amy L; Vaughan, George H; Banerjee, Shailendra N

    2016-01-15

    From 1990 to 2004, the reported rates of diarrheal disease (three or more loose stools or a greater than normal frequency in a 24-hour period) on cruise ships decreased 2.4%, from 29.2 cases per 100,000 travel days to 28.5 cases (1,2). Increased rates of acute gastroenteritis illness (diarrhea or vomiting that is associated with loose stools, bloody stools, abdominal cramps, headache, muscle aches, or fever) occurred in years that novel strains of norovirus, the most common etiologic agent in cruise ship outbreaks, emerged (3). To determine recent rates of acute gastroenteritis on cruise ships, CDC analyzed combined data for the period 2008-2014 that were submitted by cruise ships sailing in U.S. jurisdiction (defined as passenger vessels carrying ≥13 passengers and within 15 days of arriving in the United States) (4). CDC also reviewed laboratory data to ascertain the causes of acute gastroenteritis outbreaks and examined trends over time. During the study period, the rates of acute gastroenteritis per 100,000 travel days decreased among passengers from 27.2 cases in 2008 to 22.3 in 2014. Rates for crew members remained essentially unchanged (21.3 cases in 2008 and 21.6 in 2014). However, the rate of acute gastroenteritis was significantly higher in 2012 than in 2011 or 2013 for both passengers and crew members, likely related to the emergence of a novel strain of norovirus, GII.4 Sydney (5). During 2008-2014, a total of 133 cruise ship acute gastroenteritis outbreaks were reported, 95 (71%) of which had specimens available for testing. Among these, 92 (97%) were caused by norovirus, and among 80 norovirus specimens for which a genotype was identified, 59 (73.8%) were GII.4 strains. Cruise ship travelers experiencing diarrhea or vomiting should report to the ship medical center promptly so that symptoms can be assessed, proper treatment provided, and control measures implemented.

  7. An outbreak of viral gastroenteritis on a cruise ship.

    Science.gov (United States)

    McEvoy, M; Blake, W; Brown, D; Green, J; Cartwright, R

    1996-12-06

    Three hundred and seventy-eight passengers reported gastroenteritis during four cruises in the western Mediterranean on consecutive weeks of 1995. The rate at which cases were reported each day increased on the fourth cruise. The ship's owner commissioned an epidemiological investigation from the PHLS Communicable Disease Surveillance Centre. Cases reported explosive vomiting and diarrhoea, which lasted from 24 hours to five days, and were suggestive of viral gastroenteritis. No food handlers reported illness, but enquiries suggested that some had been ill and treated themselves. No bacterial pathogens were isolated from faecal specimens provided by cases or from water, food, and environmental samples taken from the galley. Small round structured viruses (SRSV) were identified by reverse transcriptase polymerase chain reaction in two faecal specimens and one specimen of vomit from people who became ill during the fourth cruise. SRSV was also identified in one faecal specimen by electron microscopy. Environmental inspection revealed inappropriate food handling, hygiene, and storage. During one 24 hour period no chlorine was detectable in the water. A case control study conducted on the fourth cruise sought details of exposure to various foodstuffs, unbottled water, and various parts of the ship. No significant associations were found between illness and any exposures. The evidence strongly suggested a continuing outbreak of SRSV infection transmitted from person to person. Some passengers remained on board for a second week and could have transmitted their infection to new arrivals. The ship was cleared and disinfected at the end of the fourth cruise in order to interrupt transmission. Fewer than 10 cases presented in each of the fifth and sixth cruises.

  8. An Assessment of Fecal Coliform Bacteria in Cruise Ship Wastewater Discharge

    Science.gov (United States)

    2003-09-01

    An Assessment of Fecal Coliform Bacteria in Cruise Ship Wastewater Discharge Charles D. McGee Orange County Sanitation District* 10844 Ellis...Alaska Cruise Ship Initiative in 1999. This initiative required investigation, understanding and oversight of discharges from large cruise ships...into the waters of Alaska. As part of the overall assessment of impacts from cruise ship waste discharges on the environment, a Science Advisory

  9. Terrain Contour Matching (TERCOM): A Cruise Missile Guidance Aid

    Science.gov (United States)

    Golden, Joe P.

    1980-12-01

    The Cruise Missile is guided by an inertial guidance system aided by an updating technique called Terrain Contour Matching (TERCOM). Chance-Vought first proposed the terrain correlation technique in the late 1950's. Since that time TERCOM has evolved into a reliable, accurate, all weather, day and night method of position fixing and updating for cruise missiles. A brief history of TERCOM development will be presented giving results where possible. A description of TERCOM and how is works will be discussed. A snapshot of the present TERCOM status and future planned developments will be addressed.

  10. Report on oceanographic cruises and data stations 2001

    OpenAIRE

    Gjertsen, Karen

    2002-01-01

    The present report shows the course tracks and stations occupied by the research vessels "G.O. Sars", "Johan Hjort", "Michael Sars" and "G.M. Dannevig" during 2001. The first tables shows the cruise number and the periods for each cruise. The next page shows the standard sections worked out by the Institute of Marine Research and the position of the fixed stations in the coast water. The following tables (page 2 and 3) indicate when the standard sections are worked out, and sho...

  11. Recombinant factor VIII in the management of hemophilia A: current use and future promise

    Directory of Open Access Journals (Sweden)

    Jerry S Powell

    2009-05-01

    Full Text Available Jerry S PowellDivision of Hematology and Oncology, University of California Davis Cancer Center, Sacramento, CA, USAAbstract: Hemophilia A is a rare inherited bleeding disorder due to mutation of the gene that encodes the coagulation protein factor VIII. Historically, prior to the availability of treatment with factor VIII preparations, most boys died from uncontrolled bleeding, either spontaneous bleeding or after injury, before reaching 20 years of age. One of the most impressive triumphs of modern medicine is that with current recombinant factor VIII replacement therapy, a boy born in the 21st century with severe hemophilia A can anticipate a normal life expectancy with essentially no permanent complications from bleeding. For severe hemophilia A, current optimal treatment should have two goals: first, to provide sufficient factor VIII to prevent spontaneous bleeding, and second, to provide sufficient factor VIII to have normal coagulation function after any trauma. However, the replacement therapy requires tremendous resources for effective use, and remains extraordinarily expensive. Thus there are opportunities for further advances in therapy for hemophilia A. Two major concerns continue to trouble current optimal treatment approaches: some patients will develop neutralizing antibodies during the first 50 infusions of therapeutic factor VIII, and second, to administer therapeutic factor VIII every other day in young boys often requires placement of a central venous access device, and such use carries the life-threatening risks of infection and thrombosis. Because of the effectiveness of current therapy, any new developments in treatment will require significant concerns for safety, both immediate and in the long term. A number of research groups seek to prolong the biological efficacy of infused recombinant factor VIII. Currently, one such promising development is in the advanced stages of clinical trial. The goals will be to improve

  12. The F309S mutation increases factor VIII secretion in human cell line

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    Daianne Maciely Carvalho Fantacini

    2016-06-01

    Full Text Available ABSTRACT OBJECTIVES: The capacity of a human cell line to secrete recombinant factor VIII with a F309S point mutation was investigated, as was the effect of the addition of chemical chaperones (betaine and sodium-4-phenylbutyrate on the secretion of factor VIII. METHODS: This work used a vector with a F309S mutation in the A1 domain to investigate FVIII production in the HEK 293 human cell line. Factor VIII activity was measured by chromogenic assay. Furthermore, the effects of chemical drugs on the culture were evaluated. RESULTS: The addition of the F309S mutation to a previously described FVIII variant increased FVIII secretion by 4.5 fold. Moreover, the addition of betaine or sodium-4-phenylbutyrate increased the secretion rate of FVIIIΔB proteins in HEK 293 cells, but the same effect was not seen for FVIIIΔB-F309S indicating that all the recombinant protein produced had been efficiently secreted. CONCLUSION: Bioengineering factor VIII expressed in human cells may lead to an efficient production of recombinant factor VIII and contribute toward low-cost coagulation factor replacement therapy for hemophilia A. FVIII-F309S produced in human cells can be effective in vivo.

  13. Desenvolvimento de inibidores do fator VIII na hemofilia A Development of factor VIII inhibitors in hemophilia A

    Directory of Open Access Journals (Sweden)

    Daniel G. Chaves

    2009-01-01

    Full Text Available A hemofilia A é uma coagulopatia genética com herança recessiva ligada ao cromossomo X que afeta 1-2 a cada 10 mil indivíduos do sexo masculino nascidos vivos. Estes indivíduos têm baixas concentrações ou ausência do fator VIII (FVIII da coagulação no plasma e apresentam quadros hemorrágicos leves, moderados e graves, dependendo da atividade de FVIII circulante. Estes pacientes necessitam de constante reposição proteica e aproximadamente 30% deles desenvolvem aloanticorpos contra a proteína exógena. A síntese dos anticorpos anti-FVIII é iniciada quando o FVIII exógeno é endocitado por células apresentadoras de antígeno, degradado e apresentado às células T CD4+ na forma de peptídeos ligados a moléculas do complexo maior de histocompatibilidade (MHC de classe II. Alguns fatores de risco (paciente/tratamento podem ser relacionados ao desenvolvimento desta resposta imune. Neste contexto, as mutações no gene do FVIII e polimorfismos em genes envolvidos na resposta imune são candidatos moleculares como determinantes imunogenéticos na predisposição para o desenvolvimento de inibidores. Por não ser completamente entendido e controlado, o desenvolvimento desta resposta imune contra o FVIII constitui o maior problema decorrente do tratamento de indivíduos portadores de hemofilia A e faz-se necessária busca de opções que visem minimizar suas ações deletérias. Algumas alternativas de tratamento têm se mostrado eficazes no tratamento (anti-CD20, plasmaférese, concentrado de complexo protrombínico (PCCs, concentrado de complexo protrombínico ativado (APCCs, fator VII humano ativado, mas a retirada ou neutralização específica dos inibidores de FVIII ainda não foram alcançadas.Hemophilia A, which affects 1-2:10,000 live-born male neonates, is a genetic coagulopathy with recessive inheritance linked to the X chromosome. These individuals have low concentrations or no coagulation factor VIII (FVIII in the plasma

  14. Samplelist of the Cicar (Cooperative Investigations in the Caribbean and Adjacent Regions) cruises 1970 – 1972

    NARCIS (Netherlands)

    Spoel, van der S.; Koperdraat, M.J.

    1974-01-01

    The Royal Dutch Navy participated in the cruises of the Cooperative Investigations in the Caribbean and Adjacent Regions (Cicar) from 1970 up to 1973 with H.Nl.M.S. “Luymes” and made 39 cruises. For general information on these cruises and preliminary results one is referred to the titles in the lis

  15. Ecological considerations in constructing marine infrastructure: The Falmouth cruise terminal development, Jamaica

    NARCIS (Netherlands)

    Korbee, D.; Mol, A.P.J.; Tatenhove, van J.P.M.

    2015-01-01

    Cruise tourism is an important and expanding global industry. The growth of this sector,coupled with the continuous development of larger cruise ships, creates demands for new marine infrastructure. The development of these marine infrastructures takes place at the intersection of global cruise tour

  16. Ecological considerations in constructing marine infrastructure: The Falmouth cruise terminal development, Jamaica

    NARCIS (Netherlands)

    Korbee, D.; Mol, A.P.J.; Tatenhove, van J.P.M.

    2015-01-01

    Cruise tourism is an important and expanding global industry. The growth of this sector,coupled with the continuous development of larger cruise ships, creates demands for new marine infrastructure. The development of these marine infrastructures takes place at the intersection of global cruise tour

  17. Ecological considerations in constructing marine infrastructure: The Falmouth cruise terminal development, Jamaica

    NARCIS (Netherlands)

    Korbee, D.; Mol, A.P.J.; Tatenhove, van J.P.M.

    2015-01-01

    Cruise tourism is an important and expanding global industry. The growth of this sector,coupled with the continuous development of larger cruise ships, creates demands for new marine infrastructure. The development of these marine infrastructures takes place at the intersection of global cruise

  18. 77 FR 65621 - Security Zone; Cruise Ships, Santa Barbara Harbor, Santa Barbara, CA

    Science.gov (United States)

    2012-10-30

    ... waters from the surface to the sea floor within a 100-yard radius of any cruise ship located within 3... prevent the catastrophic impact that a terrorist attack against a cruise ship would have on the public... to the sea floor within a 100-yard radius of any cruise ship which is located within 3 nautical...

  19. 77 FR 36955 - Security Zone; Cruise Ships, Santa Barbara Harbor, Santa Barbara, CA

    Science.gov (United States)

    2012-06-20

    ... cruise ship located within 3 nautical miles of the Santa Barbara Harbor Breakwater Light (Light List... impact a terrorist attack against a cruise ship would have on the public interest, the Coast Guard... sea floor within a 100-yard radius of any cruise ship which is located within 3 nautical miles...

  20. 76 FR 4833 - Security Zones; Cruise Ships, Port of San Diego, CA

    Science.gov (United States)

    2011-01-27

    ... waters within a 100 yard radius around any cruise ship that is located within the San Diego port area... to provide for the safety of the cruise ship, vessels, and users of the waterway. Entry into these... floor, within a 100 yard radius around any cruise ship that is within the San Diego port area inside...

  1. 76 FR 15216 - Security Zones; Cruise Ships, Port of San Diego, CA

    Science.gov (United States)

    2011-03-21

    ... any cruise ship that is located within the San Diego port area landward of the sea buoys bounding the... provide for the safety of the cruise ship, vessels, and users of the waterway. Entry into these security... any cruise ship that is within the San Diego port area inside the sea buoys bounding the Port of...

  2. 76 FR 50710 - Security Zones; Cruise Ships, San Pedro Bay, CA

    Science.gov (United States)

    2011-08-16

    ... radius around any cruise ship that is located within the San Pedro Bay port area landward of the sea... the safety of the cruise ship, vessels, and users of the waterway. Entry into these security zones... the sea floor, within a 100-yard radius around any cruise ship in the following locations is...

  3. 77 FR 1025 - Security Zones; Cruise Ships, San Pedro Bay, CA

    Science.gov (United States)

    2012-01-09

    ... zones to encompass only navigable waters within a 100-yard radius around any cruise ship that is located... necessary to provide for the safety of the cruise ship, vessels, and users of the waterway. Entry into these..., within a 100 yard radius around any cruise ship that is within the San Pedro Bay port area inside the...

  4. Generation of an optimized lentiviral vector encoding a high-expression factor VIII transgene for gene therapy of hemophilia A.

    Science.gov (United States)

    Johnston, J M; Denning, G; Doering, C B; Spencer, H T

    2013-06-01

    We previously compared the expression of several human factor VIII (fVIII) transgene variants and demonstrated the superior expression properties of B domain-deleted porcine fVIII. Subsequently, a hybrid human/porcine fVIII molecule (HP-fVIII) comprising 91% human amino-acid sequence was engineered to maintain the high-expression characteristics of porcine fVIII. The bioengineered construct then was used effectively to treat knockout mice with hemophilia A. In the current study, we focused on optimizing self-inactivating (SIN) lentiviral vector systems by analyzing the efficacy of various lentiviral components in terms of virus production, transduction efficiency and transgene expression. Specifically, three parameters were evaluated: (1) the woodchuck hepatitis post-transcriptional regulatory element (WPRE), (2) HIV versus SIV viral vector systems and (3) various internal promoters. The inclusion of a WPRE sequence had negligible effects on viral production and HP-fVIII expression. HIV and SIV vectors were compared and found to be similar with respect to transduction efficiency in both K562s and HEK-293T cells. However, there was an enhanced expression of HP-fVIII by the SIV system, which was evident in both K562 and BHK-M cell lines. To further compare expression of HP-fVIII from an SIV-based lentiviral system, we constructed expression vectors containing the high expression transgene and a human elongation factor-1 alpha, cytomegalovirus (CMV) or phosphoglycerate kinase promoter. Expression was significantly greater from the CMV promoter, which also yielded therapeutic levels of HP-fVIII in hemophilia A mice. Based on these studies, an optimized vector contains the HP-fVIII transgene driven by a CMV internal promoter within a SIV-based lentiviral backbone lacking a WPRE.

  5. Hemodialysis in a patient with severe hemophilia A and factor VIII inhibitor.

    Science.gov (United States)

    Gopalakrishnan, Natarajan; Usha, Thiruvengadam; Thopalan, Balasubramaniyan; Dhanapriya, Jeyachandran; Dineshkumar, Thanigachalam; Thirumalvalavan, Kaliaperumal; Sakthirajan, Ramanathan

    2016-10-01

    Hemophilia A is a hereditary X-linked recessive disease caused by mutations in the gene encoding factor VIII (FVIII), occurring in 1 out of 10,000 persons. Life expectancy and quality of life have dramatically improved recently in patients with hemophilia. Chronic kidney disease and need for renal replacement therapy in these patients are rare. The development of inhibitors to FVIII is the most serious complication of hemophilia and makes treatment of bleeds very challenging. We describe here a 28-year-old male patient with severe hemophilia A with presence of factor VIII inhibitor, who had end stage renal disease. Central venous access device was inserted along with infusion of factor eight inhibitor bypass activity before and after the procedure. He is currently on thrice weekly hemodialysis and doing well for 6 months without bleeding episodes. To our knowledge, hemophilia A with factor VIII inhibitor managed with hemodialysis has not been reported so far. © 2016 International Society for Hemodialysis.

  6. The Joint Cruise Missiles Project: An Acquisition History--Appendixes

    Science.gov (United States)

    1982-08-01

    21 TERCOM and Terrain Following Operation.......................22 DMA/JCMPO Organizational Interactions...50 MRASM (AGM-1091) Test Program................................52 Cruise Missile TERCOM and Survivability Flight Tests...data package TEL Transporter erector launcher T&E Test and evaluation TEMP Test and evaluation master plan TERCOM Terrain contour matching UFC Unit

  7. 78 FR 51728 - Fees for Sanitation Inspections of Cruise Ships

    Science.gov (United States)

    2013-08-21

    ... Prevention. Appendix A Size/Cost Factors Used to Determine Inspection Fees Impacts Approximate Vessel size... HUMAN SERVICES Centers for Disease Control and Prevention Fees for Sanitation Inspections of Cruise Ships AGENCY: Centers for Disease Control and Prevention (CDC), Department of Health and Human Services...

  8. 77 FR 12843 - Fees for Sanitation Inspections of Cruise Ships

    Science.gov (United States)

    2012-03-02

    ... Prevention. Appendix A ] Size/Cost Factor Approximate cost per GRT Vessel size GRT \\1\\ (in U.S. dollars... HUMAN SERVICES Centers for Disease Control and Prevention Fees for Sanitation Inspections of Cruise Ships AGENCY: Centers for Disease Control and Prevention (CDC), Department of Health and Human Services...

  9. The potential of Connecterd Cruise Control in the Netherlands

    NARCIS (Netherlands)

    Klunder, G.A.; Jonkers, E.; Woldeab, Z.T.

    2013-01-01

    This paper presents the in-car advisory system Connected Cruise Control. The system gives advice – which is determined in a central back office based on the traffic state – to the driver, in order to prevent or solve suboptimal traffic flow conditions. The system aims at optimal lane distribution in

  10. Learning to Work on a Cruise Ship: Accounts from Bali

    Science.gov (United States)

    Artini, Luh Putu; Nilan, Pam

    2014-01-01

    This article looks at motivations and both formal and informal learning contexts for well-educated young Balinese from poorer areas who enrol in cruise ship training colleges. The major motivations were getting a high income and helping the family. While basic hospitality and tourism skills are acquired, trainees also named other capacities such…

  11. Controlling The Spread of Land-Attack Cruise Missiles.

    Science.gov (United States)

    2007-11-02

    Debate in the ’New’ European Community," Arms Control Today 23, no. 2 (March 1993): 12. 38 Controlling the Spread of Land-Attack Cruise Missiles...of temperature, pressures or atmosphere in autoclaves or hydro- claves when used for the production of composites or partially processed composites

  12. Contact infection of infectious disease onboard a cruise ship

    Science.gov (United States)

    Zhang, Nan; Miao, Ruosong; Huang, Hong; Chan, Emily Y. Y.

    2016-12-01

    Cruise tourism has become more popular. Long-term personal contact, complex population flows, a lack of medical care facilities, and defective infrastructure aboard most cruise ships is likely to result in the ship becoming an incubator for infectious diseases. In this paper, we use a cruise ship as a research scenario. Taking into consideration personal behavior, the nature and transfer route of the virus across different surfaces, virus reproduction, and disinfection, we studied contact infection of infectious disease on a cruise ship. Using gastroenteritis caused by the norovirus as an example, we analyzed the characteristics of infectious disease propagation based on simulation results under different conditions. We found hand washing are the most important factors affecting virus propagation and passenger infection. It also decides either the total number of virus microorganisms or the virus distribution in different functional areas. The transfer rate between different surfaces is a key factor influencing the concentricity of the virus. A high transfer rate leads to high concentricity. In addition, the risk of getting infected is effectively reduced when the disinfection frequency is above a certain threshold. The efficiency of disinfection of functional areas is determined by total virus number and total contact times of surfaces.

  13. Cooperative adaptive cruise control: An artificial potential field approach

    NARCIS (Netherlands)

    Semsar-Kazerooni, E.; Verhaegh, J.; Ploeg, J.; Alirezaei, M.

    2016-01-01

    In this paper, in addition to the main functionality of vehicle following, cooperative adaptive cruise control (CACC) is enabled with additional features of gap closing and collision avoidance. Due to its nonlinear nature, a control objective such as collision avoidance, cannot be addressed using a

  14. Field Report from the Nalunaq Cruise March/April 2001

    DEFF Research Database (Denmark)

    Asmund, G.

    The purpose of the cruise was to collect environmental baseline samples in the Saqqaa Fjord outside of the Kirkespir Valley in South Greenland, where a gold mine is planed. Such a study should be performed in three different years during the same annual period in order to assess satisfying...

  15. Energy and exergy analysis of a cruise ship

    DEFF Research Database (Denmark)

    Baldi, Francesco; Ahlgren, Fredrik; Nguyen, Tuong-Van

    2015-01-01

    to its final use on board. To illustrate this, we perform ed an analysis of the energy and exergy flow rates of a cruise ship sailing in the Baltic Sea based on a combination of available measurements from ship operations and of mechanistic knowledge of the system . The energy analys is allows...

  16. Cruise Ships: Continuity and Change in the World System

    Directory of Open Access Journals (Sweden)

    Francisca Oyogoa

    2016-03-01

    Full Text Available Cruise ships present a useful context to study contemporary developments in globalization.  U.S.-owned cruise companies have managed to create the “ideal” context for contemporary corporations: very little government oversight of labor relations, an available pool of very cheap labor dispersed across the globe, lax environmental regulations, high profit margins, and corporate tax rates around 1%.  A typical cruise ship leaving the U.S. contains workers from 75 to 90 nationalities.  Crewmembers performing menial service work are recruited exclusively from “poor countries” in Asia, Eastern Europe, Latin America, and the Caribbean. Crewmembers typically sign 10-month contracts stipulating 10-14 hour workdays/7 days a week without vacation or sick days. There is a striking correlation between workers’ pay/status and their countries’ position within the world system.  Staff members are usually white Westerners, while crewmembers are exclusively from the global south. On cruises the legacies of imperialism and colonialism are often the basis of workers’ racialization as appropriate servants.

  17. Learning to Work on a Cruise Ship: Accounts from Bali

    Science.gov (United States)

    Artini, Luh Putu; Nilan, Pam

    2014-01-01

    This article looks at motivations and both formal and informal learning contexts for well-educated young Balinese from poorer areas who enrol in cruise ship training colleges. The major motivations were getting a high income and helping the family. While basic hospitality and tourism skills are acquired, trainees also named other capacities such…

  18. Physiological effects of Adaptive Cruise Control behaviour in real driving

    NARCIS (Netherlands)

    Brouwer, A.M.; Snelting, A.F.; Jaswa, M.; Flascher, O.; Krol, L.R.; Zander, T.O.

    2017-01-01

    We examined physiological responses to behavior of an Adaptive Cruise Control (ACC) system during real driving. ACC is an example of automating a task that used to be performed by the user. In order to preserve the link between the user and an automated system such that they work together optimally,

  19. Drevnerusskij pamflet na Mihaila VIII Paleologa v svete germenevtičeskogo analiza

    Directory of Open Access Journals (Sweden)

    Demincev Mihail

    2015-01-01

    Full Text Available In the article the perception of the image of Michael VIII Palaeologus by literate citizens of Tsardom of Russia is considered. The study is based on analysis of writings that date back to XVII century. The study focuses on identifying the symbols and latent ideas, that point to a semantic connection of the studied writings with The Old Testament, Apocalypse and some antique texts. In the end of the article the authjr makes a conclusion, that the perception of the image of Michael VIII Palaeologus in Tsardom of Russia was negative.

  20. Immunological abnormalities in haemophilia: are they caused by American factor VIII concentrate?

    Science.gov (United States)

    Froebel, K S; Madhok, R; Forbes, C D; Lennie, S E; Lowe, G D; Sturrock, R D

    1983-10-15

    Scottish patients with haemophilia, most of whom had received no American factor VIII concentrate for over two years, were found to have immunological abnormalities similar to those in their American counterparts--that is, a reduced proportion of T helper cells, an increased proportion of T suppressor cells, and a reduced response to concanavilin A. Factor VIII from both the United States and Scotland severely inhibited the in vitro lymphocyte response to mitogens in patients and controls. The American and Scottish concentrates could not be distinguished in terms of either patient usage or their effect in vitro. These results argue against a disease vector specific to American blood products.

  1. Variation in baseline factor VIII concentration in a retrospective cohort of mild/moderate hemophilia A patients carrying identical F8 mutations

    NARCIS (Netherlands)

    Loomans, J.I.; Velzen, A.S. van; Eckhardt, C.L.; Peters, M.; Makipernaa, A.; Holmstrom, M.; Brons, P.P.T.; Dors, N.; Haya, S.; Voorberg, J.; Bom, J.G. Van Der; Fijnvandraat, K.

    2017-01-01

    Essentials Factor VIII levels vary in mild and moderate hemophilia A (MHA) patients with the same mutation. We aimed to estimate the variation and determinants of factor VIII levels among MHA patients. Age and genotype explain 59% of the observed inter-individual variation in factor VIII levels.

  2. Factor VIII and von Willebrand factor changes during normal pregnancy and puerperium.

    Science.gov (United States)

    Sánchez-Luceros, Analía; Meschengieser, Susana S; Marchese, Carlos; Votta, Roberto; Casais, Patricia; Woods, Adriana I; Nadal, María V; Salviú, María J; Lazzari, María A

    2003-10-01

    Gestation is a challenge to haemostasis and it is associated with significant haemostatic changes. Several studies have evaluated von Willebrand factor in normal pregnancy, but none considered the personal history of bleeding. We studied a group of healthy non-bleeding women (184 pregnant, 64 puerperium, 37 non-pregnant) to evaluate normal ranges and their relationship to blood group and parity. The von Willebrand factor increased markedly from non-pregnant values up to the end of early puerperium (P < 0.0001), while factor VIII only showed a slight increase. Factor VIII and von Willebrand factor activity remained within the normal range for non-pregnant women. The return to non-pregnant factor levels occurred in late puerperium, later than previously reported. Only factor VIII was significantly lower in the O blood group (P = 0.035). As regards parity, there were no differences in factor VIII, von Willebrand factor antigen and von Willebrand factor ristocetin cofactor between primigravidae and multigravidae for any period studied (P = 0.888, 0.999, and 0.237, respectively). Our results provide reference ranges that may help to design a study in von Willebrand factor disease in pregnancy.

  3. Quark matter coupled to domain walls in Bianchi types II, VIII and IX Universes

    Indian Academy of Sciences (India)

    S D Katore; M M Sancheti; S P Hatkar

    2014-10-01

    In this study of Bianchi types II, VIII and IX Universes, quark matter coupled to domain walls in the context of general relativity are explored. To obtain deterministic solution of the Einstein’s field equations, various techniques are adopted. The features of the obtained solution are discussed.

  4. Challenges and Opportunities of Information Technology in the 90s. Track VIII: Managing Distributed Computing Services.

    Science.gov (United States)

    CAUSE, Boulder, CO.

    Six papers from the 1990 CAUSE Conference Track VIII: Managing Distributed Computing are presented. Authors discuss the challenges and opportunities involved in providing user managers with direct access to institutional databases to support their decision making and planning activities. Papers and their authors are as follows: "Rendering an…

  5. Storage and regulated secretion of factor VIII in blood outgrowth endothelial cells

    NARCIS (Netherlands)

    van den Biggelaar, M.; Bouwens, E.A.M.; Kootstra, N.A.; Hebbel, R.P.; Voorberg, J.; Mertens, K.

    2009-01-01

    Background Gene therapy provides an attractive alternative for protein replacement therapy in hemophilia A patients. Recent studies have shown the potential benefit of directing factor (F)VIII gene delivery to cells that also express its natural carrier protein von Willebrand factor (VWF). In this

  6. Factor VIII and von Willebrand factor co-delivery by endothelial cells

    NARCIS (Netherlands)

    Bouwens, E.A.M.|info:eu-repo/dai/nl/314061894

    2011-01-01

    A defect in coagulation factor VIII (FVIII) results in the inherited bleeding disorder hemophilia A. Current treatment of hemophilia A is hampered by the need of frequent administration of costly FVIII products. Therefore gene therapy is an attractive alternative for protein replacement to treat

  7. Safety and Efficacy of BAY 94-9027, a Prolonged-Half-Life Factor VIII

    DEFF Research Database (Denmark)

    Reding, M T; Ng, H J; Poulsen, Lone Hvitfeldt

    2016-01-01

    BACKGROUND: BAY 94-9027 is a B-domain-deleted prolonged-half-life recombinant factor VIII (FVIII) conjugates in a site-specific manner with polyethylene glycol. OBJECTIVE: Assess efficacy and safety of BAY 94-9027 for prophylaxis and treatment of bleeds in patients with severe hemophilia A PATIEN...

  8. Aspectos literarios de la obra de don Joan de Castellanos : Capitulo VIII

    Directory of Open Access Journals (Sweden)

    Mario Germán Romero

    1966-03-01

    Full Text Available Capítulo VIII. Los discursos de Jiménez de Quesada. Toda historia de la literatura colombiana comienza con el nombre del licenciado don Gonzalo Jiménez de Quesada. Que era un letrado, no puede ponerse en duda. Los escritos que de él conocemos justifican plenamente el título.

  9. Storage and regulated secretion of factor VIII in blood outgrowth endothelial cells

    NARCIS (Netherlands)

    van den Biggelaar, M.; Bouwens, E.A.M.; Kootstra, N.A.; Hebbel, R.P.; Voorberg, J.; Mertens, K.

    2009-01-01

    Background Gene therapy provides an attractive alternative for protein replacement therapy in hemophilia A patients. Recent studies have shown the potential benefit of directing factor (F)VIII gene delivery to cells that also express its natural carrier protein von Willebrand factor (VWF). In this s

  10. Immune recognition and processing of blood coagulation factor VIII by antigen-presenting cells

    NARCIS (Netherlands)

    van Haren, S.D.

    2011-01-01

    Hemophilia A is an X chromosome-linked bleeding disorder caused by a reduction or com­plete absence of coagulation factor VIII (FVIII). The bleeding tendency in patients suffering from hemophilia A can be treated by regular intravenous administrations of FVIII. A severe complication that occurs in

  11. Factor VIII and von Willebrand factor co-delivery by endothelial cells

    NARCIS (Netherlands)

    Bouwens, E.A.M.

    2011-01-01

    A defect in coagulation factor VIII (FVIII) results in the inherited bleeding disorder hemophilia A. Current treatment of hemophilia A is hampered by the need of frequent administration of costly FVIII products. Therefore gene therapy is an attractive alternative for protein replacement to treat hem

  12. Storage and regulated secretion of factor VIII in blood outgrowth endothelial cells

    NARCIS (Netherlands)

    van den Biggelaar, M.; Bouwens, E.A.M.; Kootstra, N.A.; Hebbel, R.P.; Voorberg, J.; Mertens, K.

    2009-01-01

    Background Gene therapy provides an attractive alternative for protein replacement therapy in hemophilia A patients. Recent studies have shown the potential benefit of directing factor (F)VIII gene delivery to cells that also express its natural carrier protein von Willebrand factor (VWF). In this s

  13. 7 CFR 1901.203 - Title VIII of the Civil Rights Act of 1968.

    Science.gov (United States)

    2010-01-01

    ..., rental, or financing of housing contained in title VIII apply to: (1) All dwellings financed by loans... equal housing opportunity throughout the nation. We encourage and support an affirmative advertising and... Public Law 103-354 programs and referral to the Department of Justice for suit by the United States...

  14. Analysis of mutations in the entire coding sequence of the factor VIII gene

    Energy Technology Data Exchange (ETDEWEB)

    Bidichadani, S.I.; Lanyon, W.G.; Connor, J.M. [Glascow Univ. (United Kingdom)] [and others

    1994-09-01

    Hemophilia A is a common X-linked recessive disorder of bleeding caused by deleterious mutations in the gene for clotting factor VIII. The large size of the factor VIII gene, the high frequency of de novo mutations and its tissue-specific expression complicate the detection of mutations. We have used a combination of RT-PCR of ectopic factor VIII transcripts and genomic DNA-PCRs to amplify the entire essential sequence of the factor VIII gene. This is followed by chemical mismatch cleavage analysis and direct sequencing in order to facilitate a comprehensive search for mutations. We describe the characterization of nine potentially pathogenic mutations, six of which are novel. In each case, a correlation of the genotype with the observed phenotype is presented. In order to evaluate the pathogenicity of the five missense mutations detected, we have analyzed them for evolutionary sequence conservation and for their involvement of sequence motifs catalogued in the PROSITE database of protein sites and patterns.

  15. Detection of two intervening Ne viii absorbers probing warm gas at z ˜ 0.6

    Science.gov (United States)

    Pachat, Sachin; Narayanan, Anand; Khaire, Vikram; Savage, Blair D.; Muzahid, Sowgat; Wakker, Bart P.

    2017-10-01

    We report on the detection of two Ne viii absorbers, at z = 0.619 07 and 0.570 52 in the Hubble Space Telescope/Cosmic Origins Spectrograph spectrum of background quasars SDSS J080908.13 + 461925.6 and SBS 1122 + 594, respectively. The Ne viii 770 line is at ∼3σ significance. In both instances, the Ne viii is found to be tracing gas with T ≳ 105 K, predominantly collisionally ionized, with moderate densities of n_{H} ≲ 10^{-4} cm-3, sub-solar metallicities and total hydrogen column densities of N(H) ≳ 1019 cm-2. In the z = 0.619 07 absorber, the low, intermediate ions and O vi are consistent with origin in photoionized gas, with the O vi potentially having some contribution from the warm collisional phase traced by Ne viii. The z = 0.570 52 system has H I absorption in at least three kinematically distinct components, with one of them having b({H I}) = 49 {± } 11 km s-1. The intermediate-ionization lines, O vi and Ne viii, are coincident in velocity with this component. Their different line widths suggest warm temperatures of T = (0.5-1.5) × 105 K. Both absorbers are residing in regions where there are several luminous (≳L★) galaxies. The absorber at z = 0.570 52 is within the virial radius of a 2.6L★ galaxy, possibly associated with shock-heated circumgalactic material.

  16. VIII Olimpíada Brasileira de Astronomia e Astronáutica

    Science.gov (United States)

    Garcia Canalle, João Batista; Villas da Rocha, Jaime Fernando; Wuensche de Souza, Carlos Alexandre; Pereira Ortiz, Roberto; Aguilera, Nuricel Villalonga; Padilha, Maria De Fátima Catta Preta; Pessoa Filho, José Bezerra; Soares Rodrigues, Ivette Maria

    2007-07-01

    Neste trabalho apresentamos as motivações pelas quais organizamos, em conjunto, pela primeira vez, a Olimpíada Brasileira de Astronomia incluindo a Astronáutica, em colaboração com a Agência Espacial Brasileira. Esta ampliação contribuiu para atrair ainda mais alunos, professores, escolas e patrocinadores para participarem desta Olimpíada. Em 2005 participaram da VIII Olimpíada Brasileira de Astronomia e Astronáutica (VIII OBA) 187.726 alunos distribuídos por 3.229 escolas, pertencentes a todos os estados brasileiros, incluindo o Distrito Federal. O crescimento em número de alunos participantes foi 52,4% maior do que em 2004. Em abril de 2005 organizamos, em Itapecerica da Serra, SP, um curso para os 50 alunos previamente selecionados e participantes da VII OBA e ao final selecionamos, dentre eles, uma equipe de 5 alunos, os quais representaram o Brasil na X Olimpíada Internacional de Astronomia, na China, em outubro de 2005. Ganhamos, pela primeira vez, uma medalha de ouro naquele evento. Em Agosto de 2005, organizamos a VIII Escola de Agosto para 50 alunos e respectivos professores, em Águas de Lindóia, SP, juntamente com a XXXI reunião anual da Sociedade Astronômica Brasileira (SAB). Em novembro de 2005 realizamos a I Jornada Espacial, em São José dos Campos, com 22 alunos e 22 professores selecionados dentre os participantes que melhores resultados obtiveram nas questões de Astronáutica da VIII OBA. Neste trabalho detalhamos os resultados da VIII OBA bem como as ações subseqüentes.

  17. The Joint Cruise Missiles Project: An Acquisition History

    Science.gov (United States)

    1982-08-01

    his staff at the Air Force ALCM System Project Office. The authors are, of course , solely responsible for the accuracy and interpretation of the...meaning) Sea launched cruise missile (current meaning) SPOC System Project Officers Council SRAM Short range attack missile STCM Supersonic tactical...Council ( SPOC ), com- posed of the directors of the four major missile systems (consisting at that time of the GLCM, ship- and sub-launched SLCM, and the

  18. Open Architecture as an Enabler for FORCEnet Cruise Missile Defense

    Science.gov (United States)

    2007-09-01

    battlespace via the FORCEnet information network. Current capability gaps include a lack of common configuration and Fire Control Quality ( FCQ ...connectivity among platforms that is needed to achieve and maintain a robust cruise missile defense. FCQ is defined as data obtained with the sufficient...platform, or a combination of the two. With Fire Control Quality ( FCQ ) threat data from a remote sensor, the remote unit can initiate a launch

  19. Impact of cruise ship emissions in Victoria, BC, Canada

    Science.gov (United States)

    Poplawski, Karla; Setton, Eleanor; McEwen, Bryan; Hrebenyk, Dan; Graham, Mark; Keller, Peter

    2011-02-01

    Characterization of the effects of cruise ship emissions on local air quality is scarce. Our objective was to investigate community level concentrations of fine particulate matter (PM 2.5), nitrogen dioxide (NO 2) and sulphur dioxide (SO 2) associated with cruise ships in James Bay, Victoria, British Columbia (BC), Canada. Data obtained over four years (2005-2008) at the nearest air quality network site located 3.5 km from the study area, a CALPUFF modeling exercise (2007), and continuous measurements taken in the James Bay community over a three-month period during the 2009 cruise ship season were examined. Concentrations of PM 2.5 and nitrogen oxide (NO) were elevated on weekends with ships present with winds from the direction of the terminal to the monitoring station. SO 2 displayed the greatest impact from the presence of cruise ships in the area. Network data showed peaks in hourly SO 2 when ships were in port during all years. The CALPUFF modeling analysis found predicted 24-hour SO 2 levels to exceed World Health Organization (WHO) guidelines of 20 μg m -3 for approximately 3% of 24-hour periods, with a maximum 24-hour concentration in the community of 41 μg m -3; however, the CALPUFF model underestimated concentrations when predicted and measured concentrations were compared at the network site. Continuous monitoring at the location in the community predicted to experience highest SO 2 concentrations measured a maximum 24-hour concentration of 122 μg m -3 and 16% of 24-hour periods were above the WHO standard. The 10-minute concentrations of SO 2 reached up to 599 μg m -3 and exceeded the WHO 10-minute SO 2 guideline (500 μg m -3) for 0.03% of 10-minute periods. No exceedences of BC Provincial or Canadian guidelines or standards were observed.

  20. Shipborne Laser Beam Weapon System for Defence against Cruise Missiles

    Directory of Open Access Journals (Sweden)

    J.P. Dudeja

    2000-04-01

    Full Text Available Sea-skim~ing cruise missiles pose the greatest threat to a surface ship in the present-day war scenario. The convenitional close-in-weapon-systems (CIWSs are becoming less reliable against these new challenges requiring extremely fast reaction time. Naval Forces see a high energy laser as a feasible andjeffective directed energy weapon against sea-skimming antiship cruise missiles becauseof its .ability to deliver destructive energy at the speed of light on to a distant target. The paper comparesthe technology and capability of deuterium fluoride (DF and chemical-oxygen-iodine laser (COIL in effectively performing the role of a shipborne CIWS altainst sea-skimming missiles. Out of these twolasers, it is argued that DF laser wo.uld be more effective a,s a shipborne weapon for defence against sea-skimmin,g cruise missiles. Besides the high energy laser as the primary (killing laser, othersub-systems required in the complete weapon system would be: A beacon laser to sense phase distor'ions in the primary laser, adaptive optics to compensate the atmospheric distortions, beam-directing optics, illuminating lasers, IRST sensors, surveillance and tracking radars, interfacing system, etc.

  1. Texas Teacher at Sea on the BOLIVAR Project Geophysical Cruise

    Science.gov (United States)

    Keelan, M.; Sullivan, S.; Ellins, K.

    2004-12-01

    UTIG provides K-12 teachers with research experiences in field programs that involve UTIG scientists. I am a 6th-9th grade science teacher in Van Vleck, Texas and in April 2004 I sailed to the southeastern Caribbean aboard the R/V Maurice Ewing as a member of the BOLIVAR Project alongside scientists from the U.S. and Venezuela. Our goal was collect seismic data to image the crust and mantle beneath the Caribbean as part of a study of the tectonic processes accompanying different stages of the Caribbean arc/South America continental collision process. Throughout the 52-day cruise I worked as a watch stander, interpreted newly collected seismic reflection data, helped deploy the streamer, maintained a cruise blog (a chronological journal weblog documenting personal thoughts about my experience), spoke with students in Texas with the telephone on loan from Iridium Satellite Solutions, and responded to email inquiries from shore-based students. It was hard work, but most importantly, a voyage of discovery. With guidance from scientists and GK-12 Fellows at UTIG, I am using my experience and the data collected as the basis for K-12 curriculum resources, including learning activities and a video documentary. Support for my participation and post-cruise activities was provided by the NSF and the Trull Foundation in Texas. If given the opportunity to do this again, I would, without reservation!

  2. Bianchi VIII and IX vacuum cosmologies: Almost every solution forms particle horizons and converges to the Mixmaster attractor

    CERN Document Server

    Brehm, Bernhard

    2016-01-01

    Bianchi models are posited by the BKL picture to be essential building blocks towards an understanding of generic cosmological singularities. We study the behaviour of spatially homogeneous anisotropic vacuum spacetimes of Bianchi type VIII and IX, as they approach the big bang singularity. It is known since 2001 that generic Bianchi IX spacetimes converge towards the so-called Mixmaster attractor as time goes towards the singularity. We extend this result to the case of Bianchi VIII vacuum. The BKL picture suggests that particle horizons should form, i.e. spatially separate regions should causally decouple. We prove that this decoupling indeed occurs, for Lebesgue almost every Bianchi VIII and IX vacuum spacetime.

  3. Maritime Security: Varied Actions Taken to Enhance Cruise Ship Security, but Some Concerns Remain

    Science.gov (United States)

    2010-04-01

    nonprobability sample of six cruise ship ports in the United States and four Coast Guard Sectors.5 We selected these locations based on the number of cruise ship...locations. The cruise lines we met with were primarily based on a nonprobability sample selected for their relative size and location. While their views...TSA) • Test technologies, practices, and techniques for passenger screening systems in the maritime environment. • Coordinate with the Coast

  4. Federated provenance of oceanographic research cruises: from metadata to data

    Science.gov (United States)

    Thomas, Rob; Leadbetter, Adam; Shepherd, Adam

    2016-04-01

    The World Wide Web Consortium's Provenance Data Model and associated Semantic Web ontology (PROV-O) have created much interest in the Earth and Space Science Informatics community (Ma et al., 2014). Indeed, PROV-O has recently been posited as an upper ontology for the alignment of various data models (Cox, 2015). Similarly, PROV-O has been used as the building blocks of a data release lifecycle ontology (Leadbetter & Buck, 2015). In this presentation we show that the alignment between different local data descriptions of an oceanographic research cruise can be achieved through alignment with PROV-O and that descriptions of the funding bodies, organisations and researchers involved in a cruise and its associated data release lifecycle can be modelled within a PROV-O based environment. We show that, at a first-order, this approach is scalable by presenting results from three endpoints (the Biological and Chemical Oceanography Data Management Office at Woods Hole Oceanographic Institution, USA; the British Oceanographic Data Centre at the National Oceanography Centre, UK; and the Marine Institute, Ireland). Current advances in ontology engineering, provide pathways to resolving reasoning issues from varying perspectives on implementing PROV-O. This includes the use of the Information Object design pattern where such edge cases as research cruise scheduling efforts are considered. PROV-O describes only things which have happened, but the Information Object design pattern allows for the description of planned research cruises through its statement that the local data description is not the the entity itself (in this case the planned research cruise) and therefore the local data description itself can be described using the PROV-O model. In particular, we present the use of the data lifecycle ontology to show the connection between research cruise activities and their associated datasets, and the publication of those data sets online with Digital Object Identifiers and

  5. Lesbians and Gay Men's Vacation Motivations, Perceptions, and Constraints: A Study of Cruise Vacation Choice.

    Science.gov (United States)

    Weeden, Clare; Lester, Jo-Anne; Jarvis, Nigel

    2016-08-01

    This study explores the push-pull vacation motivations of gay male and lesbian consumers and examines how these underpin their perceptions and purchase constraints of a mainstream and LGBT(1) cruise. Findings highlight a complex vacation market. Although lesbians and gay men share many of the same travel motivations as their heterosexual counterparts, the study reveals sexuality is a significant variable in their perception of cruise vacations, which further influences purchase constraints and destination choice. Gay men have more favorable perceptions than lesbians of both mainstream and LGBT cruises. The article recommends further inquiry into the multifaceted nature of motivations, perception, and constraints within the LGBT market in relation to cruise vacations.

  6. DAMPAK POSITIF DAN NEGATIF SOSIAL MEDIA TERHADAP PENDIDIKAN AKHLAK ANAK (Studi Kasus di SMP Negeri 2 Kelas VIII Banda Aceh)

    National Research Council Canada - National Science Library

    NISA KHAIRUNI

    2016-01-01

    The author examines the positive and negative effects of social media on the moral education of children in school SMPN 2 class VIII Banda Aceh, it is very interesting because of the many children who abuse social media...

  7. KEEFEKTIFAN PAIRED STORYTELLING DAN JIGSAW DALAM PENINGKATAN KOMPETENSI BERBICARA SISWA KELAS VIII SMP NEGERI 3 SLEMAN

    Directory of Open Access Journals (Sweden)

    Nurmiyati Nurmiyati

    2014-10-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui: (1 apakah ada perbedaan keefektifan antara teknik paired storytelling, jigsaw, dan konvensional dalam meningkatkan kompetensi berbicara siswa, (2 teknik pembelajaran berbicara yang paling efektif antara ketiga teknik penelitian itu dalam meningkatkan kompetensi berbicara siswa kelas VIII SMP Negeri 3 Sleman. Penelitian ini menggunakan jenis penelitian eksperimen semu dengan nonequivalent control-group design. Hasil penelitian menunjukkan bahwa: (1 Ada perbedaan keefektifan antara teknik paired storytelling, jigsaw, and konvensional itu dalam meningkatkan kompetensi berbicara siswa dengan nilai F sebesar 3,532 dan signifikan dengan P < 0,05, yaitu sebesar 0,033, dan (2 teknik paired storytelling adalah teknik keterampilan berbicara yang paling efektif diantara ketiga teknik itu dalam meningkatkan kompetensi berbicara siswa kelas VIII SMP Negeri 3 Sleman dengan mean tertinggi sebesar 74,3492. Kata kunci: teknik paired storytelling, teknik jigsaw, teknik konvensional, kompetensi berbicara

  8. Treatment of radiation osteomyelitis of the mandible in a patient with acquired factor VIII inhibitor

    Energy Technology Data Exchange (ETDEWEB)

    Arimoto, Takamasa; Kishimoto, Hiromitsu; Matsumoto, Suwako; Kawanaka, Masao; Urabe, Masahiro; Yoshioka, Wataru [Hyogo Coll. of Medicine, Nishinomiya (Japan)

    1996-07-01

    A case of radiation osteomyelitis of mandible in a 56-year-old man with acquired factor VIII inhibitor is presented. He had undergone radiation therapy for malignant lymphoma of the tonsilar region seven years earlier, and also had received steroids to treat acquired factor VIII inhibitor for a year. On initial examination, he was given a diagnosis of phlegmon of the cheek caused by pericoronitis of the left lower wisdom tooth. To prevent the recurrence of inflammation, the wisdom tooth was extracted carefully, but radiation osteomyelitis occurred in association with a pathological fracture of the condylar head. After mini-pulse therapy with methylpredonisolone, segmental mandibulectomy and reconstruction with a titanium metal plate were performed. The postoperative course was satisfactory with no complications such as bleeding or infection. (author)

  9. Compact invariant sets of the Bianchi VIII and Bianchi IX Hamiltonian systems

    Energy Technology Data Exchange (ETDEWEB)

    Starkov, Konstantin E., E-mail: konst@citedi.mx [CITEDI-IPN, Av. del Parque 1310, Mesa de Otay, Tijuana, BC (Mexico)

    2011-08-22

    In this Letter we prove that all compact invariant sets of the Bianchi VIII Hamiltonian system are contained in the set described by several simple linear equalities and inequalities. Moreover, we describe invariant domains in which the phase flow of this system has no recurrence property and show that there are no periodic orbits and neither homoclinic, nor heteroclinic orbits contained in the zero level set of its Hamiltonian. Similar results are obtained for the Bianchi IX Hamiltonian system. -- Highlights: → Zero level set of Hamiltonian of Bianchi VIII/IX systems contains no periodic orbits. → Similar conditions for homoclinic/heteroclinic orbits are given. → General nonexistence conditions of compact invariant sets are got.

  10. Detection of Intracellular Factor VIII Protein in Peripheral Blood Mononuclear Cells by Flow Cytometry

    Directory of Open Access Journals (Sweden)

    Gouri Shankar Pandey

    2013-01-01

    Full Text Available Flow cytometry is widely used in cancer research for diagnosis, detection of minimal residual disease, as well as immune monitoring and profiling following immunotherapy. Detection of specific host proteins for diagnosis predominantly uses quantitative PCR and western blotting assays. In this study, we optimized a flow cytometry-based detection assay for Factor VIII protein in peripheral blood mononuclear cells (PBMCs. An indirect intracellular staining (ICS method was standardized using monoclonal antibodies to different domains of human Factor VIII protein. The FVIII protein expression level was estimated by calculating the mean and median fluorescence intensities (MFI values for each monoclonal antibody. ICS staining of transiently transfected cell lines supported the method's specificity. Intracellular FVIII protein expression was also detected by the monoclonal antibodies used in the study in PBMCs of five blood donors. In summary, our data suggest that intracellular FVIII detection in PBMCs of hemophilia A patients can be a rapid and reliable method to detect intracellular FVIII levels.

  11. FUNDAMENTALS OF AMHARIC (REVISED EDITION), UNITS VIII-IX. DIALOGUES FOR CONVERSATION, AMHARIC SCRIPT.

    Science.gov (United States)

    BARTON, DONALD K.; AND OTHERS

    THIS IS THE THIRD AND FINAL VOLUME OF A NINE-UNIT COURSE IN BASIC AMHARIC. VOLUMES ONE AND TWO DEAL WITH THE PROBLEMS OF PRONUNCIATION AND THE ORAL-AURAL MASTERY OF BASIC GRAMMATICAL CONSTRUCTIONS. AT THE END OF EACH VOLUME ARE APPENDED ENGLISH-AMHARIC AND AMHARIC-ENGLISH GLOSSARIES. VOLUME THREE (UNITS VIII-IX) IS DIVIDED INTO TWO PARTS. PART I…

  12. Construction of a mouse model of factor VIII deficiency by gene targeting

    Energy Technology Data Exchange (ETDEWEB)

    Bi, L.; Lawler, A.; Gearhart, J. [Univ. of Pennsylvania School of Medicine, Philadelphia, PA (United States)] [and others

    1994-09-01

    To develop a small animal model of hemophilia A for gene therapy experiments, we set out to construct a mouse model for factor VIII deficiency by gene targeting. First, we screened a mouse liver cDNA library using a human FVIII cDNA probe. We cloned a 2.6 Kb partial mouse factor VIII cDNA which extends from 800 base pairs of the 3{prime} end of exon 14 to the 5{prime} end of exon 26. A mouse genomic library made from strain 129 was then screened to obtain genomic fragments covering the exons desired for homologous recombination. Two genomic clones were obtained, and one covering exon 15 through 22 was used for gene targeting. To make gene targeting constructs, a 5.8 Kb genomic DNA fragment covering exons 15 to 19 of the mouse FVIII gene was subcloned, and the neo expression cassette was inserted into exons 16 and 17 separately by different strategies. These two constructs were named MFVIIIC-16 and MFVIIIC-17. The constructs were linearized and transfected into strain 129 mouse ES cells by electroporation. Factor VIII gene-knockout ES cell lines were selected by G-418 and screened by genomic Southern blots. Eight exon 16 targeted cell lines and five exon 17 targeted cell lines were obtained. Three cell lines from each construct were injected into blastocysts and surgically transferred into foster mothers. Multiple chimeric mice with 70-90% hair color derived from the ES-cell genotype were seen with both constructs. Germ line transmission of the ES-cell genotype has been obtained for the MFVIIIC-16 construct, and multiple hemophilia A carrier females have been identified. Factor VIII-deficient males will be conceived soon.

  13. Historical intensity VIII earthquakes along the Rhone valley (Valais, Switzerland): primary and secondary effects

    Energy Technology Data Exchange (ETDEWEB)

    Fritsche, S.; Faeh, D.; Schwarz-Zanetti, G.

    2012-06-15

    In recent years the upper Rhone Valley has been one of the most intensively investigated regions by the Swiss Seismological Service. The high seismicity in the region encourages research in the seismological field and one main focus has been historical seismology. This report presents the state of the art of our historical investigations by giving an overview of the effects of four damaging earthquakes with intensity larger than VII, for which a fairly large number of documents could be found and analyzed. The overview includes the events of 1584 (Aigle, epicentral intensity VIII), 1755 (Brig, epicentral intensity VIII), 1855 (Visp, epicentral intensity VIII), and 1946 (Sierre, epicentral intensity VIII for the main shock and intensity VII for the largest aftershock). The paper focuses mainly on primary and secondary effects in the epicentral region, providing the key data and a general characterization of the event. Generally, primary effects such as the reaction of the population and impact on buildings took more focus in the past. Thus building damage is more frequently described in historic documents. However, we also found a number of sources describing secondary effects such as landslides, snow avalanches, and liquefaction. Since the sources may be useful, we include citations of these documents. The 1584 Aigle event, for example, produced exceptional movements in the Lake of Geneva, which can be explained by an expanded sub aquatic slide with resultant tsunami and seiche. The strongest of the aftershocks of the 1584 event triggered a destructive landslide covering the villages Corbeyrier and Yvorne, Vaud. All macroseismic data on the discussed events are accessible through the web page of the Swiss Seismological Service (http://www.seismo.ethz.ch). (authors)

  14. Potential role of a new PEGylated recombinant factor VIII for hemophilia A

    OpenAIRE

    2016-01-01

    Tung Thanh Wynn,1 Burak Gumuscu,2,3 1Department of Pediatrics, Division of Pediatric Hematology/Oncology, University of Florida, Gainesville, FL, 2Pediatric Hematology-Oncology, Bon Secours Health System, St. Mary’s Hospital, Richmond, VA, 3Department of Pediatrics, Division of Pediatric Hematology/Oncology, University of Virginia, Charlottesville, VA, USA Abstract: Hemophilia A, a deficiency in the activity of coagulation factor (F) VIII, is an X-linked bleeding disorder with an ap...

  15. Immunogenicity and pharmacokinetic studies of recombinant Factor VIII containing lipid cochleates

    OpenAIRE

    Kosloski, Matthew P.; Peng, Aaron; Varma, Prashant R.; Fathallah, Anas M.; MICLEA, RAZVAN D.; Mager, Donald E.; Balu-Iyer, Sathy V.

    2010-01-01

    Replacement therapy using recombinant factor VIII (rFVIII) is currently the most common therapy for hemophilia A, a bleeding disorder caused by the deficiency of FVIII. However, 15–30% of patients develop inhibitory antibodies against administered rFVIII which complicates the therapy. Encapsulation or association of protein with lipidic structures can reduce this immune response. Previously, we developed and characterized rFVIII-containing phosphatidylserine (PS) cochleate cylinders using bio...

  16. A population pharmacokinetic model for perioperative dosing of factor VIII in hemophilia A patients

    Science.gov (United States)

    Hazendonk, Hendrika; Fijnvandraat, Karin; Lock, Janske; Driessens, Mariëtte; van der Meer, Felix; Meijer, Karina; Kruip, Marieke; Gorkom, Britta Laros-van; Peters, Marjolein; de Wildt, Saskia; Leebeek, Frank; Cnossen, Marjon; Mathôt, Ron

    2016-01-01

    The role of pharmacokinetic-guided dosing of factor concentrates in hemophilia is currently a subject of debate and focuses on long-term prophylactic treatment. Few data are available on its impact in the perioperative period. In this study, a population pharmacokinetic model for currently registered factor VIII concentrates was developed for severe and moderate adult and pediatric hemophilia A patients (FVIII levels hemophilia A patients by Bayesian adaptive dosing. PMID:27390359

  17. Laparoscopic Liver Resection Using the Lateral Approach from Intercostal Ports in Segments VI, VII, and VIII.

    Science.gov (United States)

    Inoue, Yoshihiro; Suzuki, Yusuke; Fujii, Kensuke; Kawaguchi, Nao; Ishii, Masatsugu; Masubuchi, Shinsuke; Yamamoto, Masashi; Hirokawa, Fumitoshi; Hayashi, Michihiro; Uchiyama, Kazuhisa

    2017-07-31

    Laparoscopic liver resection (LLR) has been developed as a minimally invasive surgery. However, challenges such as difficulty securing visibility and limited control of forceps make it difficult to complete LLR in hepatic segments VI, VII, and VIII. To overcome these challenges, we devised a surgical technique using intercostal ports. We termed this approach the lateral approach. This work describes our experience performing LLR using this approach and discusses the safety and effectiveness of this approach. Between April 2011 and December 2016, data from 91 patients who underwent LLR with or without the intercostal port at a single institution were retrospectively analyzed regarding surgical outcomes, safety, and utility. LLR was performed for 32 patients with the intercostal port and for 59 patients without the intercostal port. The conversion rates to open surgery with and without intercostal ports were 3.1 and 25.4% (P = 0.008). In hepatic segments VII and VIII, the rates of conversion to open surgery were significantly lower for cases involving intercostal ports (6.7 vs. 42.9 and 0 vs. 38.9%; P = 0.035 and 0026, respectively); however, there were no differences in hepatic segment VI (0 vs. 7.4%; P = 0.563). There were no differences in operative time, blood loss volume, surgical margin, curative resection rate, or postoperative complication rate for LLR in all segments (VI, VII, and VIII). No adverse events due to placement of the intercostal port were observed in this set of patients. LLR using the lateral approach and intercostal ports for hepatic segments VII and VIII resulted in a significant decrease in conversion rates to open surgery.

  18. Neonatal helper-dependent adenoviral vector gene therapy mediates correction of hemophilia A and tolerance to human factor VIII.

    Science.gov (United States)

    Hu, Chuhong; Cela, Racel G; Suzuki, Masataka; Lee, Brendan; Lipshutz, Gerald S

    2011-02-01

    Neonatal gene therapy is a promising strategy for treating a number of congenital diseases diagnosed shortly after birth as expression of therapeutic proteins during postnatal life may limit the pathologic consequences and result in a potential "cure." Hemophilia A is often complicated by the development of antibodies to recombinant protein resulting in treatment failure. Neonatal administration of vectors may avoid inhibitory antibody formation to factor VIII (FVIII) by taking advantage of immune immaturity. A helper-dependent adenoviral vector expressing human factor VIII was administered i.v. to neonatal hemophilia A knockout mice. Three days later, mice produced high levels of FVIII. Levels declined rapidly with animal growth to 5 wk of age with stable factor VIII expression thereafter to >1 y of age. Decline in factor VIII expression was not related to cell-mediated or humoral responses with lack of development of antibodies to capsid or human factor VIII proteins. Subsequent readministration and augmentation of expression was possible as operational tolerance was established to factor VIII without development of inhibitors; however, protective immunity to adenovirus remained.

  19. Structural characterization of the novel aminoglycoside phosphotransferase AphVIII from Streptomyces rimosus with enzymatic activity modulated by phosphorylation

    Energy Technology Data Exchange (ETDEWEB)

    Boyko, Konstantin M., E-mail: kmb@inbi.ras.ru [Bach Institute of Biochemistry, Federal Research Centre of Biotechnology of the Russian Academy of Sciences, Leninsky Prospekt. 33, Bld. 2, 119071, Moscow (Russian Federation); National Research Center “Kurchatov Institute”, Kurchatov Complex of NBICS-technologies, Akad. Kurchatova sqr., 1, Moscow, 123182 (Russian Federation); Gorbacheva, Marina A.; Korzhenevskiy, Dmitry A. [National Research Center “Kurchatov Institute”, Kurchatov Complex of NBICS-technologies, Akad. Kurchatova sqr., 1, Moscow, 123182 (Russian Federation); Alekseeva, Maria G.; Mavletova, Dilara A.; Zakharevich, Natalia V.; Elizarov, Sergey M.; Rudakova, Natalia N.; Danilenko, Valery N. [Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkina str. 3, Moscow, 119333 (Russian Federation); Popov, Vladimir O. [Bach Institute of Biochemistry, Federal Research Centre of Biotechnology of the Russian Academy of Sciences, Leninsky Prospekt. 33, Bld. 2, 119071, Moscow (Russian Federation); National Research Center “Kurchatov Institute”, Kurchatov Complex of NBICS-technologies, Akad. Kurchatova sqr., 1, Moscow, 123182 (Russian Federation)

    2016-09-02

    Aminoglycoside phosphotransferases represent a broad class of enzymes that promote bacterial resistance to aminoglycoside antibiotics via the phosphorylation of hydroxyl groups in the latter. Here we report the spatial structure of the 3′-aminoglycoside phosphotransferase of novel VIII class (AphVIII) solved by X-ray diffraction method with a resolution of 2.15 Å. Deep analysis of APHVIII structure and its comparison with known structures of aminoglycoside phosphotransferases of various types reveals that AphVIII has a typical two-domain fold and, however, possesses some unique characteristics that distinguish the enzyme from its known homologues. The most important difference is the presence of the activation loop with unique Ser146 residue. We demonstrate that in the apo-state of the enzyme the activation loop does not interact with other parts of the enzyme and seems to adopt catalytically competent state only after substrate binding. - Highlights: • 3D structure of the novel aminoglycoside phosphotransferase AphVIII was obtained. • AphVIII activation loop is clearly identified in the electron density. • AphVIII has some unique structural features in its substrate C-ring binding pocket.

  20. Pengembangan perangkat pembelajaran matematika SMP kelas VIII semester genap dengan model problem-based learning

    Directory of Open Access Journals (Sweden)

    Maria Trisna Sero Wondo

    2017-06-01

    Abstract This study aimed to produce mathematics learning kits using the problem based learning model oriented to the learning achievement, problem solving skill, and self-confidence for Grade VIII students of the junior high school (JHS in the even semester. This study was and development study used the development model by Plomp (preliminary study, development, evaluation. The research subjects were teachers and students of Grade VIII of Swasta Katolik Maria Goretti Ende. The analysis of the data was done by converting the quantitative data in the form scores as the results of the evaluation into qualitative data in the form of five-scale standard scores. This study produce mathematics learning kits in the form of lesson plans and student worksheets for Grade VIII students of the junior high school in the even semester. Of the results of expert validation, a teacher evaluation scores, a students evaluation scores, an observation for the implementation of learning activities, a learning achievement test, a problem solving skil test, and a self-confidence questionnaire show that lesson plans (RPP  and student worksheets (LKS is valid, practical, and effective. Keywords: learning kits, problem-based learning, learning achievement, problem solving, self-confidence

  1. [Structure and function of the factor VIII/von Willebrand factor complex].

    Science.gov (United States)

    Müller, G

    1990-03-01

    In the blood plasma factor VIII is bound to the von Willebrand factor. The primary structure of the two proteins were clarified by gene clonation. Factor VIII descends from a precursor protein with 2,351 amino acids by splitting of 19 amino acid residues and is activated by partial proteolysis. In the blood coagulation factor VIII acts as co-factor for the activation of factor X by factor IX in the presence of phospholipids and Ca++ within the intrinsic coagulation system. The formation of the von Willebrand factor takes place by splitting of 22 and 741 amino acid residues, respectively, from pre-pro-von Willebrand factor via pro-von Willebrand factor. The subunits of the von Willebrand factor consist od 2,050 amino acid residues. In the blood plasma the von Willebrand factor is existing as a mixture of multimeres. Receptors of the von Willebrand factor on the thrombocytic membrane are the glycoproteins GPIb and GPIIb/GPIIIa, by means of which the adhesion of thrombocytes at the subendoethelium of the vascular wall and the aggregation of thrombocytes are mediated.

  2. HTLV-III seropositivity in European haemophiliacs exposed to Factor VIII concentrate imported from the USA.

    Science.gov (United States)

    Melbye, M; Froebel, K S; Madhok, R; Biggar, R J; Sarin, P S; Stenbjerg, S; Lowe, G D; Forbes, C D; Goedert, J J; Gallo, R C

    1984-12-22

    77 Scottish haemophiliacs and 22 Danish haemophiliacs were serologically tested for antibodies to human T-cell leukaemia virus III (HTLV-III). Since 1979 the Scottish patients had been treated largely with factor VIII concentrate produced in Scotland, whereas all but 2 of the Danish patients had received both locally prepared concentrate and commercial concentrate made from US donor material. 15.6% of Scottish and 59.1% of Danish haemophiliacs were antibody positive (p less than 0.001). None of 11 haemophiliacs not treated in the period 1979-84 was seropositive. 2 (6.7%) of 30 subjects who had been treated with locally produced concentrate only were antibody positive, compared with 23 (39.7%) of 58 subjects who had been treated with commercial concentrate. Among 52 users of both commercially and locally produced factor VIII concentrate, seropositivity was directly correlated with the consumption of commercial concentrate (p less than 0.001) but not locally produced material. These data indicate that European haemophiliacs were exposed to HTLV-III via some factor VIII concentrates obtained from the USA.

  3. Renal Vein Thrombosis in a Newborn With Abnormal Factor VIII Level: Clinical Case Report.

    Science.gov (United States)

    Szafranska, Agnieszka; Pajak, Agata; Kilis-Pstrusinska, Katarzyna; Królak-Olejnik, Barbara

    2015-08-01

    Renal vein thrombosis (RVT) in neonates is a rare condition of low mortality but significant morbidity due to renal impairment.We report the case of a male term newborn with left RVT and elevated serum factor VIII (FVIII).The main symptoms of the patient and the important clinical findings: prompt diagnosis of RVT was possible because the classic clinical presentation of macroscopic hematuria, thrombocytopenia, and palpable flank mass were present in this newborn infant.The main diagnoses: finally, the reason of RVT was established when the infant was 3 months of age: the increased level of FVIII was confirmed. We discuss the diagnosis, therapy, and outcome of the patient and compare with the literature.Therapeutics interventions: however, despite anticoagulant therapy the left kidney developed areas of scarring and then atrophy.Conclusions and outcomes: Prothrombotic defects should be considered in all patients with perinatal RVT. Elevated factor VIII as a reason of RVT in neonatal period is particularly rare. Given a poor renal outcome in children associated with elevated levels of factor VIII, consideration could be given to more aggressive antithrombotic therapy in such cases.

  4. EFFECT OF ORAL CONTRACEPTIVES (LD AND CILEST ON CLOTTING FACTORS VIII AND IX

    Directory of Open Access Journals (Sweden)

    H.R. Sadeghipour Roudsari

    1997-10-01

    Full Text Available Based on epidemiologic data, women who take oral contraceptives seem to have an increased risk of developing thromboembollic disease. The thrombotic effects of oral contraceptive (OC are probably mediated, at least partly through their effects on the coagulation system. Plasma levels of several clotting factors have been shown to be elevated in OC users, and this increase is graduated according to the dose of estrogen. In this study, fifty healthy and non smoking women, aged 18-35 years, were randomly assigned to treatment with 2 different OCs: a monophasic pill containing 30 pg of ethinyl estradiol plus 150µg levonorgestrel (LD and a monophasic pill containing 35µg ethinylestradiol plus 250pg norgestimate (Cilest. Factor VIII plasma values were significantly decreased (P<0.05 only in women treated with the preparation LD, but the levels of factor VIII were not significantly different in the group treated with Cilest. Factor IX plasma values were significantly increased (P<0.05 only in women treated with the preparation Cilest, but the levels of factor Ix were not significantly different in the group treated with LD. In LD and cilest users factors VIII and IX were not significantly changed (P<0.05 in overweight and obese subjects in comparison to normal weight.

  5. [Medicine aboard cruise ships--law insurance specifics].

    Science.gov (United States)

    Ottomann, C; Frenzel, R; Muehlberger, T

    2013-04-01

    The booming cruise industry, associated with ships with more passengers and crew on board, results in growing medical needs for the ship doctor. The ship's doctor insurance policy includes different jurisdictions, namely national law, international law, tort law, insurance law and labor law. In addition, international agreements must be taken into account, which complicates the design of an adequate insurance policy. Equally high are the costs and defense costs for the ship's doctor in case of liability. In order to limit the liability for all parties is to ask for appropriately qualified medical staff, hired on board.

  6. Flying qualities design criteria applicable to supersonic cruise aircraft

    Science.gov (United States)

    Chalk, C. R.

    1980-01-01

    A comprehensive set of flying qualities design criteria was prepared for use in the supersonic cruise research program. The framework for stating the design criteria is established and design criteria are included which address specific failures, approach to dangerous flight conditions, flight at high angle of attack, longitudinal and lateral directional stability and control, the primary flight control system, and secondary flight controls. Examples are given of lateral directional design criteria limiting lateral accelerations at the cockpit, time to roll through 30 deg of bank, and time delay in the pilot's command path. Flight test data from the Concorde certification program are used to substantiate a number of the proposed design criteria.

  7. Preliminary Report of Kagoshima Maru Cruise Ocean Navigation in 2006

    OpenAIRE

    福田, 隆二; 益満, 侃; 内山, 正樹; 東, 隆文; 有田, 洋一; 吉永, 圭輔; フクダ, リュウジ; マスミツ, スナオ; ウチヤマ, マサキ; アズマ, タカフミ; アリタ, ヨウイチ; ヨシナガ, ケイスケ; FUKUDA, Ryuji; Masumitsu, Sunao; UCHIYAMA, Masaki

    2006-01-01

    The Kagoshima-maru cruise, Ocean Navigation 2006, was conducted in the period from August 9 to September 28, 2006. The oceanographic surveys along a line on the sea were carried in August 25-27 over the fishing area in the eastern part of Indian Ocean. XBT observations were conducted along the line every one degree in longitude and shipboard observations were ADCP conducted along the entire line. Two warm eddies were found along the line, based on a map of the sea surface height anomaly. Th...

  8. Year 2000 small engine technology payoffs in cruise missiles

    Science.gov (United States)

    Singh, B.; Benstein, E. H.

    1986-01-01

    A study has been conducted for advanced small (450-850 pounds thrust) gas turbine engines for a subsonic strategic cruise missile application, using projected year-2000 technology. Engine performance and configuration analyses were performed for two and three spool turbofan and propfan engine concepts. Mission and Life Cycle Cost (LCC) analyses were performed in which the candidate engines were compared to the baseline engine over a prescribed mission. The advanced technology engines reduced system LCC up to 41 percent relative to the baseline engine. The critical aerodynamic materials and mechanical systems necessary for turbine engine technology were identified.

  9. Tracklines of Multichannel Seismics Survey Collected During USGS Cruise M1-98-GM (GOM98LINE.SHP)

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Shapefile showing tracklines of Multichannel Seismics Survey Collected During USGS Cruise M1-98-GM. This cruise was to the Mississippi Canyon region of the Gulf of...

  10. PENINGKATAN PEMBELAJARAN MENULIS PUISI BEBAS MELALUI MEDIA LAGU MEDLEY SISWA KELAS VIII SMP SINTANG

    Directory of Open Access Journals (Sweden)

    Rohanawati Silalahi

    2015-05-01

    Full Text Available Penelitian ini bertujuan untuk meningkatkan pembelajaran menulis puisi bebas melalui media lagu medley siswa kelas VIII B SMP Sintang. Subjek penelitian adalah siswa kelas VIII B SMP Sintang. Penelitian ini merupakan Penelitian Tindakan Kelas (PTK. Prosedur pelaksanaan dan implementasi penelitian dilaksanakan dalam dua siklus. Teknik pengumpulan data berupa obsevasi, wawancara, catatan lapangan, angket, evaluasi dan dokumentasi. Instrumen pengumpulan data meliputi lembar wawancara, lembar catatan lapangan, lembar angket, lembar tes hasil belajar dan lembar portofolio serta dokumentasi foto kegiatan. Teknik analisis data yang digunakan adalah analisis data kuantitatif dan kualitatif. Hasil penelitian ini menunjukkan bahwa penggunaan media lagu medley dapat meningkatkan pembelajaran menulis puisi bebas. Peningkatan hasil atau produk dapat dilihat dari perbandingan skor rata-rata keterampilan menulis puisi bebas siswa pada setiap siklus. Nilai rata-rata siswa pada tahap pratindakan adalah 55,23, pada saat siklus I meningkat menjadi 71,18, dan pada siklus II meningkat menjadi 73,4. ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ DEVELOPING THE TEACHING OF FREE VERSE WRITING THROUGH MEDLEY SONG MEDIA TO GRADE VIII STUDENTS OF SMP SINTANG   Abstract This study aims to improve the teaching of free verse writing through medley song media to Grade VIII B students of SMP Sintang. The research subjects were Grade VIII B students of SMP Sintang,. This was a classroom action research (CAR. The research procedure and implementation were conducted in two cycles. The data were collected through observations, field notes, questionnaires, evaluations, and documentations. The data collecting instruments included interview sheets, field note sheets

  11. Socio-cultural impacts of large-scale cruise tourism in Souq Mutrah, Sultanate of Oman

    Directory of Open Access Journals (Sweden)

    Manuela Gutberlet

    2016-02-01

    Full Text Available The following paper explores socio-cultural impacts of large-scale cruise liner tourism on the traditional bazaar (souq in the district of Mutrah. The souq is located opposite the port in the Omani capital Muscat. Large-scale cruise tourism in Muscat started only in 2004 and has increased in scale and numbers in the past years. 24 cruise vessels with around 7600 passengers arrived in Muscat in 2005. Seven years later 135 cruise liners carrying 257,000 tourists docked in Muscat. Due to this dramatic rise of international cruise ships, the socio-cultural impacts have increased for local residents, shop vendors/owners and tourists alike. To capture those socio-cultural impacts on Souq Mutrah, a survey of cruise tourists was conducted by a questionnaire. In addition, the researcher used participatory observation, counting, and in-depth interviews with different stakeholders of the local community and different types of tourists during the cruise seasons 2012/13 and 2013/14. Moreover, content analysis of statistics and local media publications were used. Results indicate that the souq has become “the core of a tourist bubble”, where crowding is a major problem and local residents avoid the place. The social carrying capacity of the souq has been reached. Omani vendors are leaving their businesses and renting their shops out to expatriates. Since contemporary cruise tourists are low spenders, expatriate shop sellers have become more aggressive.

  12. 76 FR 31350 - Cruise Vessel Safety and Security Act of 2010, Available Technology

    Science.gov (United States)

    2011-05-31

    ... SECURITY Coast Guard Cruise Vessel Safety and Security Act of 2010, Available Technology AGENCY: Coast... technology to meet certain provisions of the Cruise Vessel Security and Safety Act of 2010(CVSSA), specifically related to video recording and overboard detection technologies. The Notice of request for...

  13. 76 FR 5732 - Security Zones; Cruise Ships, Port of San Diego, CA; Correction

    Science.gov (United States)

    2011-02-02

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA87 Security Zones; Cruise Ships, Port of San Diego, CA... FR 4833), regarding security zones for cruise ships in the Port of San Diego, California. This... Diego, Coast Guard; telephone 619-278-7261, e-mail Michael.B.Dolan@uscg.mil . If you have questions on...

  14. 33 CFR 165.1108 - Security Zones; Cruise Ships, Port of San Diego, California.

    Science.gov (United States)

    2010-07-01

    ..., Port of San Diego, California. 165.1108 Section 165.1108 Navigation and Navigable Waters COAST GUARD... § 165.1108 Security Zones; Cruise Ships, Port of San Diego, California. (a) Definition. “Cruise ship” as... or at a port of call in the San Diego port. (b) Location. The following areas are security zones: (1...

  15. Bibliography of Supersonic Cruise Research (SCR) program from 1980 to 1983

    Science.gov (United States)

    Hoffman, S.

    1984-01-01

    A bibliography for the Supersonic Cruise Research (SCR) and Variable Cycle Engine (VCE) Programs is presented. An annotated bibliography for the last 123 formal reports and a listing of titles for 44 articles and presentations is included. The studies identifies technologies for producing efficient supersonic commercial jet transports for cruise Mach numbers from 2.0 to 2.7.

  16. 33 CFR 105.290 - Additional requirements-cruise ship terminals.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Additional requirements-cruise ship terminals. 105.290 Section 105.290 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MARITIME SECURITY MARITIME SECURITY: FACILITIES Facility Security Requirements § 105.290 Additional requirements—cruise ship...

  17. 77 FR 29254 - Safety Zones, Large Cruise Ships; Lower Mississippi River, Southwest Pass Sea Buoy to Mile Marker...

    Science.gov (United States)

    2012-05-17

    ... extends from bank to bank encompassing one-mile ahead and one-mile astern of each large cruise ship. This... ship as it transits the Lower Mississippi River between the Port of New Orleans Cruise Ship Terminal.... For the purpose of this rule, the term ``large cruise ship'' is defined as a vessel over 100 feet...

  18. The influence of cruise control and adaptive cruise control on driving behaviour--a driving simulator study.

    Science.gov (United States)

    Markvollrath; Schleicher, Susanne; Gelau, Christhard

    2011-05-01

    Although Cruise Control (CC) is available for most cars, no studies have been found which examine how this automation system influences driving behaviour. However, a relatively large number of studies have examined Adaptive Cruise Control (ACC) which compared to CC includes also a distance control. Besides positive effects with regard to a better compliance to speed limits, there are also indications of smaller distances to lead vehicles and slower responses in situations that require immediate braking. Similar effects can be expected for CC as this system takes over longitudinal control as well. To test this hypothesis, a simulator study was conducted at the German Aerospace Center (DLR). Twenty-two participants drove different routes (highway and motorway) under three different conditions (assisted by ACC, CC and manual driving without any system). Different driving scenarios were examined including a secondary task condition. On the one hand, both systems lead to lower maximum velocities and less speed limit violations. There was no indication that drivers shift more of their attention towards secondary tasks when driving with CC or ACC. However, there were delayed driver reactions in critical situations, e.g., in a narrow curve or a fog bank. These results give rise to some caution regarding the safety effects of these systems, especially if in the future their range of functionality (e.g., ACC Stop-and-Go) is further increased.

  19. Crystal Structure of Human Factor VIII: Implications for the Formation of the Factor IXa-Factor VIIIa Complex

    Energy Technology Data Exchange (ETDEWEB)

    Ngo, J.C.; Huang, M.; Roth, D.A.; Furie, B.C.; Furie, B. (Wyeth); (MBL)

    2008-06-03

    Factor VIII is a procofactor that plays a critical role in blood coagulation, and is missing or defective in hemophilia A. We determined the X-ray crystal structure of B domain-deleted human factor VIII. This protein is composed of five globular domains and contains one Ca{sup 2+} and two Cu{sup 2+} ions. The three homologous A domains form a triangular heterotrimer where the A1 and A3 domains serve as the base and interact with the C2 and C1 domains, respectively. The structurally homologous C1 and C2 domains reveal membrane binding features. Based on biochemical studies, a model of the factor IXa-factor VIIIa complex was constructed by in silico docking. Factor IXa wraps across the side of factor VIII, and an extended interface spans the factor VIII heavy and light chains. This model provides insight into the activation of factor VIII and the interaction of factor VIIIa with factor IXa on the membrane surface.

  20. Crystal Structure of Human Factor VIII: Implications for the Formation of the Factor IXa-Factor VIIIa Complex

    Energy Technology Data Exchange (ETDEWEB)

    Chi Ki Ngo,J.; Huang, M.; Roth, D.; Furie, B.; Furie, B.

    2008-01-01

    Factor VIII is a procofactor that plays a critical role in blood coagulation, and is missing or defective in hemophilia A. We determined the X-ray crystal structure of B domain-deleted human factor VIII. This protein is composed of five globular domains and contains one Ca(2+) and two Cu(2+) ions. The three homologous A domains form a triangular heterotrimer where the A1 and A3 domains serve as the base and interact with the C2 and C1 domains, respectively. The structurally homologous C1 and C2 domains reveal membrane binding features. Based on biochemical studies, a model of the factor IXa-factor VIIIa complex was constructed by in silico docking. Factor IXa wraps across the side of factor VIII, and an extended interface spans the factor VIII heavy and light chains. This model provides insight into the activation of factor VIII and the interaction of factor VIIIa with factor IXa on the membrane surface.

  1. AKTIVITAS FISIK, ASUPAN ENERGI DAN STATUS GIZI WANITA PEMETIK TEH DI PTPN VIII BANDUNG, JAWA BARAT

    Directory of Open Access Journals (Sweden)

    Venny Agustiani Mahardikawati

    2012-03-01

    Full Text Available 800x600 Normal 0 false false false IN X-NONE X-NONE MicrosoftInternetExplorer4 Quality of human resources is very important to improve productivity. The worker productivity is correlated with nutritional status and health status. The objective of the research is to analyze physical activity, energy intake, nutritional status, of women workers at Tea Plantation PTPN VIII Bandung, West Java. The cross sectional design was used in this study to elaborate physical activity, nutritional status, and productivity of tea picker’s women. The criteria of study sample were tea picker’s women at cluster area of Malabar tea plantation of PTPN VIII Bandung, having infant and they were willing to be interviewed. The total number of 92 women sample was chosen randomly. Primary data consisted of physical activity recall (2x24 hours, food consumption recall (2x24 hours, anthropometry data (weight and height, and productivity (passage of tea sprout. Secondary data were included data of PT Perkebunan Nusantara VIII Bandung, West Java. The result showed that more than a half of samples having active or moderate physical activity level (PAL. The physical activity level of samples during work day (average PAL=1.87 was higher than holiday (average PAL=1.69 (p2362 kcal and 2134 kcal. The average energy adequacy level during workday and day off according to Schofield and Oxford equation were not significantly different, respectively 97,2% and 103,3%. The majority of samples had normal nutritional status, and 30.4% of the sample was overweight. The energy adequacy level according to Schofield and Oxford equation are related to nutritional status of women workers. Keywords: physical activity, energy expenditure, nutritional status, plantation women

  2. Refreezing previously thawed fresh-frozen plasma. Stability of coagulation factors V and VIII:C.

    Science.gov (United States)

    Dzik, W H; Riibner, M A; Linehan, S K

    1989-09-01

    With the growth in autologous blood programs and the increased scrutiny of the indications for transfusion of fresh-frozen plasma (FFP), an increase has been seen in the number of occasions on which FFP was requested and thawed but then not transfused. The coagulation properties of FFP units that were refrozen and then rethawed were therefore studied. Fifty-eight units of plasma were studied, with each experimental unit of FFP paired with an identical control unit. Experimental units were frozen, stored at -65 degrees C, thawed, stored at 1 to 6 degrees C for various periods of time up to 24 hours, and then refrozen, stored at -65 degrees C, rethawed, and stored again in the refrigerator for up to 24 hours. Control units were frozen once at the time the experimental units were first frozen and thawed once at the time of the second thaw of the experimental units. Aliquots of plasma were sampled periodically and were later batch-tested for prothrombin time (PT), activated partial thromboplastin time (aPTT), and factor V and VIII:C activity. The results of coagulation testing of the twice-frozen plasmas were always within the normal range. There was a slight but statistically valid prolongation of the PT and aPTT and a decrease in the factor V and VIII:C levels for twice-frozen plasma compared with control plasma. The greatest decline occurred in the level of factor VIII:C. The measured deterioration in coagulation of twice-frozen FFP is unlikely to be of clinical importance. Refreezing FFP may eventually prove useful for rare donor, autologous, and massive transfusion programs.

  3. Analysis of inversions in the factor VIII gene in Spanish hemophilia A patients and families

    Energy Technology Data Exchange (ETDEWEB)

    Domenech, M.; Tizzano, E.; Baiget, M. [Hospital de Sant Pau, Barcelona (Spain); Altisent, C. [Hospital Vall d`Hebron, Barcelona (Spain)

    1994-09-01

    Intron 22 is the largest intron of the factor VIII gene and contains a CpG island from which two additional transcripts originate. One of these transcripts corresponds to the F8A gene which have telomeric extragenic copies in the X chromosome. An inversion involving homologous recombination between the intragenic and the distal or proximal copies of the F8A gene has been recently described as a common cause of severe hemophilia A (HA). We analyzed intron 22 rearrangements in 195 HA patients (123 familial and 72 sporadic cases). According to factor VIII levels, our sample was classified as severe in 114 cases, moderate in 29 cases and mild in 52 cases. An intron 22 (F8A) probe was hybridized to Southern blots of BcII digested DNA obtained from peripheral blood. A clear pattern of altered bands identifies distal or proximal inversions. We detected an abnormal pattern identifying an inversion in 49 (25%) of the analyzed cases. 43% of severe HA patients (49 cases) showed an inversion. As expected, no inversion was found in the moderate and mild group of patients. We found a high proportion (78%) of the distal rearrangement. From 49 identified inversions, 33 were found in familial cases (27%), while the remaining 15 were detected in sporadic patients (22%) in support that this mutational event occurs with a similar frequency in familial or sporadic cases. In addition, we detected a significant tendency of distal inversion to occur more frequently in familial cases than in sporadic cases. Inhibitor development to factor VIII was documented in approximately 1/3 of the patients with inversion. The identification of such a frequent molecular event in severe hemophilia A patients has been applied in our families to carrier and prenatal diagnosis, to determine the origin of the mutation in the sporadic cases and to detect the presence of germinal mosaicism.

  4. L'Italia unita...dalle infrastrutture

    Directory of Open Access Journals (Sweden)

    Stefania Maffeo

    2011-04-01

    Full Text Available The events that led to the proclamation of the Reign of Italy in 1861, after the heroic actions by Garibaldi and the wise politics by Cavour, opened a new stage in Italian history. This is an important moment for a country that had always lived divisions among different political parties and had always been dominated by foreign rulers. The Italian state was created in an important historical period made of technical and economical development thanks to several technological inventions, such as textile machines, the train, the electrical telegraph. These inventions needed also a development in communications. Soon after the Unity of Italy the conditions of the last ones were considerably backward. From the physical geographical viewpoint, the distance between the two extremes of the peninsula, leaving out also the two main isles, was longer than that of all the other European Countries. Besides, just in the Po Valley and along few flat coast lines the relief conditions were suitable for building roads, railways and modern highways. Currently all that is clear: there a few stretches without flyovers, bridges, tunnels and viaducts, which have greatly contributed to transform the landscape (for example the link Genoa – Savona, belonging to highway Fiori – Genova/Ventimiglia – opened in 1967, includes 158 km built along the coast with 27 galleries and 90 viaducts. Historically the “entries of Italy” were the alpine passes and the ports on the coast. The “Belpaese” has been perhaps the most famous example of what the geographer Jean Gottmann called the “crossroads regions” (carrefours with openings to the world which have given them great opportunities to connect and exchange. In the globalization age  the new entries to the world are the international airports, while the telematic networks contribute to get rid of the idea of border itself. A journey to discover the thick and wide realization of communications and telecommunications works which, in different ways, have created “ the united and connected Italy”, with a huge effort being greater than the one of the other European Countries, for historical, geographical and urban planning reasons. Networks being fundamental not only for the economic growth and development, but also to build the national society and conscience.

  5. The popular resort port of cruise tourism in the Eastern Mediterranean Basin: Turkey

    Directory of Open Access Journals (Sweden)

    Okan Yaşar

    2012-02-01

    Full Text Available Cruise tourism is in most demand in Europe and North America. The Eastern Mediterranean, and consequently Turkey rank among the destinations that cruise ship tour companies prefer to travel. Part of the demand for cruise tourism, which has been displaying a constantly raising momentum in tourism movements in the world, is now being directed towards Turkey. Nonetheless, the cruise tourism trend is spreading and prompting domestic demand in Turkey as well. A significant boom in the domestic demand has led to the commencement of cruise tours to the Black Sea, the Greek Islands, the Dalmatian shores and Italy leaving from Istanbul, Izmir and Kusadasi harbors. Turkey’s active foreign demand for cruise tourism mostly focuses on ports and its surrounding supply of services. Cruise tourism is now one of the predominant agendas within policies for diversifying and spreading tourism. Within one year Turkey managed to be ranked among the first ten countries in regard to the number of tourists and tourism revenues in the world.

  6. The popular resort port of cruise tourism in the Eastern Mediterranean Basin: Turkey

    Directory of Open Access Journals (Sweden)

    Okan Yasar

    2012-02-01

    Full Text Available Cruise tourism is in most demand in Europe and North America. The Eastern Mediterranean, and consequently Turkey rank among the destinations that cruise ship tour companies prefer to travel. Part of the demand for cruise tourism, which has been displaying a constantly raising momentum in tourism movements in the world, is now being directed towards Turkey. Nonetheless, the cruise tourism trend is spreading and prompting domestic demand in Turkey as well. A significant boom in the domestic demand has led to the commencement of cruise tours to the Black Sea, the Greek Islands, the Dalmatian shores and Italy leaving from Istanbul, Izmir and Kusadasi harbors. Turkey’s active foreign demand for cruise tourism mostly focuses on ports and its surrounding supply of services. Cruise tourism is now one of the predominant agendas within policies for diversifying and spreading tourism. Within one year Turkey managed to be ranked among the first ten countries in regard to the number of tourists and tourism revenues in the world.       

  7. Characterization of Chinese Hamster Ovary Cells Producing Coagulation Factor VIII Using Multi-omics Tools

    DEFF Research Database (Denmark)

    Kaas, Christian Schrøder

    ,000 fold over the last couple of years due to the revolution of next-generation sequencing (NGS), has dramatically accelerated CHO-omics from virtually non-existent to a vibrant growing field. The aim of this thesis was to investigate the impact of coagulation factor VIII (FVIII) production in CHO cells...... for analysis and engineering of industrially relevant CHO cells. Full implementation of such tools for generating specifically engineered CHO production cell lines may allow significant cost-reductions in production of complex biopharmaceuticals such as FVIII....

  8. Precise Calculations of Astrophysically Important Allowed and Forbidden Transitions of Xe VIII

    Science.gov (United States)

    Bhowmik, Anal; Nath Dutta, Narendra; Roy, Sourav

    2017-02-01

    The present work reports transition line parameters for Xe viii, which are potentially important for astrophysics in view of recent observations of multiply ionized xenon in hot white dwarfs. The relativistic coupled-cluster method is employed here to calculate the E1, E2, and M1 transition line parameters with high accuracy. The E1 oscillator strengths and probabilities of E2 and M1 transitions are determined using theoretical amplitudes and experimental energy values. The calculated branching ratios and the lifetimes are supplemented to the transition parameters. The accurate presentation of these calculated data is crucial for density estimation in several stellar and interstellar media.

  9. Precise calculations of astrophysically important allowed and forbidden transitions of Xe VIII

    CERN Document Server

    Bhowmik, Anal; Roy, Sourav

    2016-01-01

    The present work reports astrophysically important transition line parameters for Xe VIII. The relativistic coupled-cluster method is employed here to calculate the E1, E2, and M1 transition line parameters with a high accuracy. The E1 oscillator strengths and probabilities of E2 and M1 transitions are determined using theoretical amplitudes and experimental energy values. The calculated branching ratios and the lifetimes are supplement to the transition parameters. The accurate presentations of these calculated data are crucial for the density estimations in several stellar and inter-stellar mediums.

  10. Isidore of Seville and comici ueteres. Ad Isid., Orig. VIII 7.7

    Directory of Open Access Journals (Sweden)

    Krzysztof Rzepkowski

    2012-12-01

    Full Text Available In chapter 7 of book VIII of Etymologies dedicated to Roman poets (De poetis, Isidore draws a dividing line between the so-called ueteres and noui comici. Among ueteres he counts Plautus, Accius, Terentius, although Accius, according to our knowledge, composed only tragedies and can hardly be considered a comic playwright. In this paper I try to prove that all the palaeographic, phonetic, historical, and literary arguments speak in favour of emending in the discussed passage the peculiar «Accius» to the expected «Caecilius» (i.e. Caecilius Statius.

  11. Underground Test Area Subproject Phase I Data Analysis Task. Volume VIII - Risk Assessment Documentation Package

    Energy Technology Data Exchange (ETDEWEB)

    None

    1996-12-01

    Volume VIII of the documentation for the Phase I Data Analysis Task performed in support of the current Regional Flow Model, Transport Model, and Risk Assessment for the Nevada Test Site Underground Test Area Subproject contains the risk assessment documentation. Because of the size and complexity of the model area, a considerable quantity of data was collected and analyzed in support of the modeling efforts. The data analysis task was consequently broken into eight subtasks, and descriptions of each subtask's activities are contained in one of the eight volumes that comprise the Phase I Data Analysis Documentation.

  12. Cruise tourism and community economic development in Central America and the Caribbean: The case of Costa Rica

    Directory of Open Access Journals (Sweden)

    Seidl, Andy

    2006-01-01

    Full Text Available This paper illustrates an economic approach to understanding the cruise tourism industry as a driver of economic development in Costa Rica. The objective is to describe the role and activities of the cruise ship industry and identify sources of economic benefit and cost such that more informed local policy decisions about the cruise ship tourism might be made. For example, our analysis indicates: the cruise tourism industry competes with the cargo shipping industry for port space at a significant cost to Costa Rican ports; the amount of money injected into the local economy per cruise tourist is substantially lower than for other types of tourism; Cruise ships purchase relatively few supplies in Costa Rica; Cruise ships generate a great deal of human waste, water and air pollution, which can create a serious health hazard, cleanup costs, and which are not commensurate with other types of tourism development available to Costa Rica; Decision makers may want to consider that investment in cruise tourism friendly ports may be less efficient from a national perspective than investment in infrastructure (e.g., airports to increase more profitable types of tourism; And leaders may want to consider the encouragement of smaller “pocket” cruises over the current cruise version of mass tourism. This approach should be applicable to communities wherever cruise tourism currently exists or is under consideration to be included in the portfolio of community economic activities

  13. Acoustic Doppler current profiling from the JGOFS Arabian sea cruises aboard the RV T.G. Thompson

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Hyun-Sook; Flagg, C.N.; Shi, Yan

    1996-06-01

    Acoustic Doppler current profiler (ADCP) data is part of the core data for the U.S. JGOFS Arabian Sea project, along with hydrographic and nutrient data. Seventeen cruises are scheduled to take place between September 1994 and January 1996 on the R/V T.G. Thompson. They are numbered consecutively from the ship`s commissioning with the first JGOFS cruise designated TN039. Table 1 lists start and end dates of each cruise with its mission. All but the first cruise have been or will be staged from Muscat, Oman. Each cruise is scheduled for a duration of between two weeks and one month. Seven of the cruises, referred to as process cruises, follow a standard cruise track, taking hydrographic, chemical and biological measurements. The rest of the cruises, which take place generally within the standard cruise region defined by a set track, are for the deployment and recovery of moored equipments and towing of a SeaSoar. ADCP data are collected using an autonomous data acquisition system developed for ship-of-opportunity cruises, named the AutoADCP system. The system is an extension of RD instrument`s DAS version 2.48 using enhancements made possible with {open_quotes}user-exit{close_quotes} programs. It makes it possible to collect ADCP data without the constant monitoring usually necessary and insures constant data coverage and uniform data quality.

  14. The role of port operations in the development of cruise tourism: The case of port of Antalya

    Directory of Open Access Journals (Sweden)

    Şirvan Şen Demir

    2016-12-01

    Full Text Available Cruise tourism, which is evaluated within marine tourism, has significant environmental, economic, and socio-cultural impacts on visited ports. Cruise tourism is an activity that provides economic income to the harbors and creates new jobs. The cruise tourism has an increasing demand in the recent years because cruise ship visitors both have an opportunity to see many countries and cities in a short time and have a vacation on a luxury ship, which has a comfort standard like a holiday village.  With her historical, cultural and natural attractions, Turkey is located in very strategic and convenient point and also Turkey is so close to destinations that cruise ships prefer to visit in the Mediterranean Basin. In 2014, approximately  22 million tourist travelled with cruise ships in the World and 1.8 million cruise passengers came to Turkey. The aim of this study is evaluate the development of cruise tourism in Turkey in terms of port operations.  In previous studies it has focused on the development of cruise tourism but has not been evaluated in terms of port operations perspective. Semi-structured interview technique was used in the study and interviewed with General Manager of Antalya Global Ports Holding, which is one of Turkey’s most important port operations. Turkey  and in the World cruise tourism is compared and has been reached important information about the development of cruise tourism.

  15. Longitudinal Control Strategy for Vehicle Adaptive Cruise Control Systems

    Institute of Scientific and Technical Information of China (English)

    WU Li-jun; LIU Zhao-du; MA Yue-feng

    2007-01-01

    A new longitudinal control strategy for vehicle adaptive cruise control (ACC) systems is presented.The running relationship between the ACC vehicle and the detected target vehicle is described by the relative velocity and the deviation between the actual headway distance and the prescribed safety distance.Based on this,two state space models are built and the linear quadratic optimal control theory is used to yield desired velocity for the ACC-equipped vehicle when with the target vehicle detected.By switching among four control modes,the desired velocity profile is designed to deal with different running situations.A velocity controller,which includes a PID controller for throttle openness and a neural network controller for brake application,is developed to achieve the desired velocity profile.The proposed control strategy is applied to a non-linear vehicle model in a simulation environment and is shown to provide the ACC vehicle comfortable ride and satisfying safety.

  16. Connected cruise control: modelling, delay effects, and nonlinear behaviour

    Science.gov (United States)

    Orosz, Gábor

    2016-08-01

    Connected vehicle systems (CVS) are considered in this paper where vehicles exchange information using wireless vehicle-to-vehicle (V2V) communication. The concept of connected cruise control (CCC) is established that allows control design at the level of individual vehicles while exploiting V2V connectivity. Due to its high level of modularity the proposed design can be applied to large heterogeneous traffic systems. The dynamics of a simple CVS is analysed in detail while taking into account nonlinearities in the vehicle dynamics as well as in the controller. Time delays that arise due to intermittencies and packet drops in the communication channels are also incorporated. The results are summarised using stability charts which allow one to select control gains to maintain stability and ensure disturbance attenuation when the delay is below a critical value.

  17. Discrete Sliding Mode Control for Hypersonic Cruise Missile

    Directory of Open Access Journals (Sweden)

    Yong Hua Fan

    2016-01-01

    Full Text Available A discrete variable structure control (DVSC with sliding vector is presented to track the velocity and acceleration command for a hypersonic cruise missile. In the design an integrator is augmented to ensure the tracking with zero steady-state errors. Furthermore the sliding surface of acceleration is designed using the error of acceleration and acceleration rate to avoid the singularity of control matrix. A proper power rate reaching law is utilized in this proposal; therefore the state trajectory from any initial point can be driven into the sliding surface. Besides, in order to validate the robustness of controller, the unmolded dynamic and parameter disturbance of the missile are considered. Through simulation the proposed controller demonstrates good performance in tracking velocity and acceleration command.

  18. Emotional processing in survivors of the Jupiter cruise ship disaster.

    Science.gov (United States)

    Joseph, S; Yule, W; Williams, R

    1995-02-01

    Twenty-three survivors of the Jupiter cruise ship disaster completed the Impact of Events Scale, a measure of intrusion and avoidance, as well as measures of arousal and affect at two points in time: between 3 and 7 months (Time 1) and between 12 and 14 months (Time 2) following the event. The aim of the present study was to explore the relationship between intrusion and avoidance and symptoms of depression and anxiety. The results suggest that although higher scores on intrusion and avoidance are strongly associated with poorer psychological outcome at each point in time, it is only intrusion which may be predictive of later symptoms. Avoidance would seem to be a response to early distress. These data are discussed with reference to a cognitive--emotional processing model of post-traumatic stress disorder.

  19. Acoustic Doppler current profiling from the JGOFS Arabian Sea cruises aboard the RV T.G. Thompson

    Energy Technology Data Exchange (ETDEWEB)

    Kim, H.S.; Flagg, C.N.; Shi, Y. [Brookhaven National Lab., Upton, NY (United States). Oceanographic and Atmospheric Sciences Div.

    1996-12-01

    Acoustic Doppler current profiler (ADCP) data is part of the core data for the US JGOFS Arabian Sea project, along with hydrographic and nutrient data. Seventeen cruises are scheduled to take place between September 1994 and January 1996 on the R/V T.G. Thompson. Seven of the cruises follow a standard cruise track, taking hydrographic, chemical and biological measurements. The rest of the cruises, which take place generally within the standard cruise region defined by a set track, are for the deployment and recovery of moored equipment and towing of a SeaSoar. Detailed description of ADCP hardware, the AutoADCP data acquisition system, and the collection of navigation and compass data on the Thompson is documented in Section 2. Followed by data collection for each cruise together with a cruise track, Section 3 presents the processing and analysis of velocity and acoustic backscatter intensity data. Section 5 shows results of profile quality diagnosis.

  20. Diatom community structure on in-service cruise ship hulls.

    Science.gov (United States)

    Hunsucker, Kelli Zargiel; Koka, Abhishek; Lund, Geir; Swain, Geoffrey

    2014-10-01

    Diatoms are an important component of marine biofilms found on ship hulls. However, there are only a few published studies that describe the presence and abundance of diatoms on ships, and none that relate to modern ship hull coatings. This study investigated the diatom community structure on two in-service cruise ships with the same cruise cycles, one coated with an antifouling (AF) system (copper self-polishing copolymer) and the other coated with a silicone fouling-release (FR) system. Biofilm samples were collected during dry docking from representative areas of the ship and these provided information on the horizontal and vertical zonation of the hull, and intact and damaged coating and niche areas. Diatoms from the genera Achnanthes, Amphora and Navicula were the most common, regardless of horizontal ship zonation and coating type. Other genera were abundant, but their presence was more dependent on the ship zonation and coating type. Samples collected from damaged areas of the hull coating had a similar community composition to undamaged areas, but with higher diatom abundance. Diatom fouling on the niche areas differed from that of the surrounding ship hull and paralleled previous studies that investigated differences in diatom community structure on static and dynamically exposed coatings; niche areas were similar to static immersion and the hull to dynamic immersion. Additionally, diatom richness was greater on the ship with the FR coating, including the identification of several new genera to the biofouling literature, viz. Lampriscus and Thalassiophysa. These results are the first to describe diatom community composition on in-service ship hulls coated with a FR system. This class of coatings appears to have a larger diatom community compared to copper-based AF systems, with new diatom genera that have the ability to stick to ship hulls and withstand hydrodynamic forces, thus creating the potential for new problematic species in the biofilm.

  1. The Impact of Cruise Ship Passengers in Maine: The Example of Bar Harbor

    OpenAIRE

    Gabe, Todd; Lynch, Colleen; McConnon, James

    2003-01-01

    Maine’s expanding cruise ship industry can provide local economic benefits and add to the state’s already large tourism economy. The authors describe results of a survey they conducted among passengers from eight ship visits to Bar Harbor, a town that has emerged as a popular port-of-call on New England summer and autumn cruises. They found that cruise ship passengers have higher household incomes and spend substantially more per day than the typical Maine tourist. They make several suggestio...

  2. QSO ABSORPTION SYSTEMS DETECTED IN Ne VIII: HIGH-METALLICITY CLOUDS WITH A LARGE EFFECTIVE CROSS SECTION

    Energy Technology Data Exchange (ETDEWEB)

    Meiring, J. D.; Tripp, T. M. [Department of Astronomy, University of Massachusetts, Amherst, MA 01003 (United States); Werk, J. K.; Prochaska, J. X. [University of California Observatories-Lick Observatory, UC Santa Cruz, CA 95064 (United States); Howk, J. C. [Department of Physics, University of Notre Dame, 225 Nieuwland Science Hall, Notre Dame, IN 46556 (United States); Jenkins, E. B. [Princeton University Observatory, Peyton Hall, Ivy Lane, Princeton, NJ 08544 (United States); Lehner, N.; Sembach, K. R. [Space Telescope Science Institute, 3700 San Martin Drive, Baltimore, MD 21218 (United States)

    2013-04-10

    Using high-resolution, high signal-to-noise ultraviolet spectra of the z{sub em} = 0.9754 quasar PG1148+549 obtained with the Cosmic Origins Spectrograph (COS) on the Hubble Space Telescope, we study the physical conditions and abundances of Ne VIII+O VI absorption line systems at z{sub abs} = 0.68381, 0.70152, 0.72478. In addition to Ne VIII and O VI, absorption lines from multiple ionization stages of oxygen (O II, O III, O IV) are detected and are well aligned with the more highly ionized species. We show that these absorbers are multiphase systems including hot gas (T Almost-Equal-To 10{sup 5.7} K) that produces Ne VIII and O VI, and the gas metallicity of the cool phase ranges from Z = 0.3 Z{sub Sun} to supersolar. The cool ( Almost-Equal-To 10{sup 4} K) phases have densities n{sub H} Almost-Equal-To 10{sup -4} cm{sup -3} and small sizes (<4 kpc); these cool clouds are likely to expand and dissipate, and the Ne VIII may be within a transition layer between the cool gas and a surrounding, much hotter medium. The Ne VIII redshift density, dN/dz{approx}7{sup +7}{sub -3}, requires a large number of these clouds for every L > 0.1 L* galaxy and a large effective absorption cross section ({approx}> 100 kpc), and indeed, we find a star-forming {approx}L {sup *} galaxy at the redshift of the z{sub abs} = 0.72478 system, at an impact parameter of 217 kpc. Multiphase absorbers like these Ne VIII systems are likely to be an important reservoir of baryons and metals in the circumgalactic media of galaxies.

  3. KEANEKARAGAMAN HYMENOPTERA PARASITOID PADA PERKEBUNAN KELAPA SAWIT PTPN VIII CINDALI, BOGOR

    Directory of Open Access Journals (Sweden)

    Ichsan Luqmana Indra Putra

    2016-09-01

    Full Text Available Diversity of parasitic Hymenoptera in PTPN VIII oil palm plantation Cindali, Bogor. One group of important natural enemies in oil palm plantation is parasitic Hymenoptera. The purpose of this research is to know the diversity and fluctuation of parasitic Hymenoptera PTPN VIII Cindali oil palm plantation. This research was conducted in 6 blocks of oil palm in September 2014 – June 2015. There were 5 plots in every observation blocks 39.2 x 39.2 m in size, and used direct and indirect method. Direct method done by 5 plants in every plots was taken randomized to observed and taken the herbivore insects to reared until the parasitic Hymenoptera came out. Observation of cover crops conducted by 3 subplots determined diagonally in every plots 9.8 x 9.8 m in size and herbivore insects was observed and collected. Indirect methods used sweep net and yellow pan trap. The result of this research, 26 parasitic Hymenoptera families was found, with the Braconidae was the most morphospecies found and the most individual amount was Scelionidae. The abundance of parasitoid in every month fluctuated.

  4. Atmospheric Imaging Assembly Response Functions: Solving the Fe VIII Problems with Hinode EIS Bright Point Data

    CERN Document Server

    Schmelz, Joan T; Kimble, Jason A; 10.1007/s11207-012-0208-1

    2013-01-01

    The Atmospheric Imaging Assembly (AIA) onboard the Solar Dynamics Observatory is a state-of-the-art imager with the potential to do unprecedented time-dependent multi-thermal analysis at every pixel on scales short compared to the radiative and conductive cooling times. Recent results, however, have identified missing spectral lines in the CHIANTI atomic physics data base, which is used to construct the instrument response functions. We have done differential emission measure analysis using simultaneous AIA and Hinode/EIS observations of six X-ray bright points. Our results not only support the conclusion that CHIANTI is incomplete near 131 angstroms, but more importantly, suggest that the peak temperature of the Fe VIII emissivity/response is likely to be closer to log T = 5.8 than to the current value of log T = 5.7. Using a revised emissivity/response calculation for Fe VIII, we find that the observed AIA 131-angstrom flux can be underestimated by about 1.25, which is smaller than previous comparisons.

  5. Many factor VIII products available in the treatment of hemophilia A: an embarrassment of riches?

    Science.gov (United States)

    Lieuw, Kenneth

    2017-01-01

    Hemophilia A (HA) is a common bleeding disorder caused by the deficiency of factor VIII (FVIII) with an incidence of ~1 in 5000 male births. Replacement of FVIII is necessary to prevent and treat bleeding episodes. However, with multiple new drugs in addition to old standards, choosing among the different FVIII treatment options is harder than ever. There are FVIII products that are plasma derived or recombinant, FVIII products designed to extend the half-life of FVIII, and the first single-chain FVIII product, recombinant factor VIII single chain (rFVIII-SC). As development of inhibitors to FVIII continues to be a major problem in the care of HA patients, recent studies showing lower rates of inhibitor development with plasma-derived FVIIII products versus recombinant FVIII products have made choosing among the many options now available even more complex. Although still unproven, extended half-life (EHL) products may provide the hope of decreased immunogenicity but need further testing in previously untreated patients (PUPs). This review highlights some of the differences between FVIII products currently available and hopefully assists the clinician to decide which FVIII product to choose for their patients.

  6. Factor V and VIII combined deficiency: clinical perioperative management for tonsillectomy in a child.

    Science.gov (United States)

    Lanchon, R; Robin, F; Brissaud, O; Marro, M; Nouette-Gaulain, K

    2014-03-01

    Combined factors V (FV) and VIII (FVIII) deficiency is a rarely seen hereditary coagulation disease. Experience of its management in surgery with a high-risk of bleeding is rare. The interest of this case report is to propose a strategy of perioperative management for such a deficit, but also to recall that a careful preoperative anesthetic evaluation with questioning and physical examination permits to detect unsuspected coagulation disorders and to schedule the preventive treatment. The protocol for the perioperative period consisted of the administration of desmopressin and fresh frozen plasma one hour before surgery. The administration of desmopressin was continued for 48hours. Fresh frozen plasma and tranexamic acid were administered during the first 9 postoperative days. A local bleeding occurred at 8 days (scab coming off) and required systematically a surgical hemostasis and an intensification of the therapeutic protocol. Recombinant plasmatic factor VIII was administered for 7 days together with a daily perfusion of fresh frozen plasma for a total treatment period of 14 days.

  7. A new recombinant factor VIII: from genetics to clinical use

    Directory of Open Access Journals (Sweden)

    Santagostino E

    2014-12-01

    Full Text Available Elena Santagostino Angelo Bianchi Bonomi Hemophilia and Thrombosis Center, Fondazione IRCCS Ca’ Granda, Ospedale Maggiore Policlinico, Milan, Italy Abstract: Advances in recombinant technology and knowledge about coagulation factor VIII (FVIII are building a platform for new therapeutic options in patients with hemophilia A. The development of turoctocog alfa, a novel, high-purity, third-generation, B-domain truncated recombinant FVIII, has been produced and formulated without the use of animal-derived or human serum-derived components, in the wake of understanding of the new biochemical characteristics of FVIII, namely its protein structure, and glycosylation and sulfating patterns. Culture conditions and a five-step purification process have been developed to optimize the safety of turoctocog alfa. The results of two pilot clinical trials using turoctocog alfa confirmed high safety levels, with no patient developing inhibitors during the period of observation. The purpose of this review is to describe briefly the molecular and biological properties of turoctocog alfa, together with details of its clinical development, with emphasis on the needs of patients with hemophilia A. Keywords: hemophilia A, recombinant factor VIII, turoctocog alfa, purification, inhibitor, safety

  8. Experimentally Derived Dielectronic Recombination Rate Coefficients for Heliumlike C V and Hydrogenic O VIII

    CERN Document Server

    Savin, D W

    1999-01-01

    Using published measurements of dielectronic recombination (DR) resonance strengths and energies for C V to C IV and O VIII to O VII, we have calculated the DR rate coefficient for these ions. Our derived rates are in good agreement with multiconfiguration, intermediate-coupling and multiconfiguration, fully-relativistic calculations as well as with most LS coupling calculations. Our results are not in agreement with the recommended DR rates commonly used for modeling cosmic plasmas. We have used theoretical radiative recombination (RR) rates in conjunction with our derived DR rates to produce a total recombination rate for comparison with unified RR+DR calculations in LS coupling. Our results are not in agreement with undamped, unified calculations for C V but are in reasonable agreement with damped, unified calculations for O VIII. For C V, the Burgess general formula (GF) yields a rate which is in very poor agreement with our derived rate. The Burgess & Tworkowski modification of the GF yields a rate w...

  9. Evaluation of B&W UO2/ThO2 VIII experimental core: criticality and thermal disadvantage factor analysis

    Energy Technology Data Exchange (ETDEWEB)

    Carlo Parisi; Emanuele Negrenti

    2017-02-01

    In the framework of the OECD/NEA International Reactor Physics Experiment (IRPHE) Project, an evaluation of core VIII of the Babcock & Wilcox (B&W) Spectral Shift Control Reactor (SSCR) critical experiment program was performed. The SSCR concept, moderated and cooled by a variable mixture of heavy and light water, envisaged changing of the thermal neutron spectrum during the operation to encourage breeding and to sustain the core criticality. Core VIII contained 2188 fuel rods with 93% enriched UO2-ThO2 fuel in a moderator mixture of heavy and light water. The criticality experiment and measurements of the thermal disadvantage factor were evaluated.

  10. Structural investigation of zymogenic and activated forms of human blood coagulation factor VIII: a computational molecular dynamics study

    Directory of Open Access Journals (Sweden)

    Venkateswarlu Divi

    2010-02-01

    Full Text Available Abstract Background Human blood coagulation factor VIII (fVIII is a large plasma glycoprotein with sequential domain arrangement in the order A1-a1-A2-a2-B-a3-A3-C1-C2. The A1, A2 and A3 domains are interconnected by long linker peptides (a1, a2 and a3 that possess the activation sites. Proteolysis of fVIII zymogen by thrombin or factor Xa results in the generation of the activated form (fVIIIa which serves as a critical co-factor for factor IXa (fIXa enzyme in the intrinsic coagulation pathway. Results In our efforts to elucidate the structural differences between fVIII and fVIIIa, we developed the solution structural models of both forms, starting from an incomplete 3.7 Å X-ray crystal structure of fVIII zymogen, using explicit solvent MD simulations. The full assembly of B-domainless single-chain fVIII was built between the A1-A2 (Ala1-Arg740 and A3-C1-C2 (Ser1669-Tyr2332 domains. The structural dynamics of fVIII and fVIIIa, simulated for over 70 ns of time scale, enabled us to evaluate the integral motions of the multi-domain assembly of the co-factor and the possible coordination pattern of the functionally important calcium and copper ion binding in the protein. Conclusions MD simulations predicted that the acidic linker peptide (a1 between the A1 and A2 domains is largely flexible and appears to mask the exposure of putative fIXa enzyme binding loop (Tyr555-Asp569 region in the A2 domain. The simulation of fVIIIa, generated from the zymogen structure, predicted that the linker peptide (a1 undergoes significant conformational reorganization upon activation by relocating completely to the A1-domain. The conformational transition led to the exposure of the Tyr555-Asp569 loop and the surrounding region in the A2 domain. While the proposed linker peptide conformation is predictive in nature and warrants further experimental validation, the observed conformational differences between the zymogen and activated forms may explain and support the

  11. 46 CFR 54.01-2 - Adoption of division 1 of section VIII of the ASME Boiler and Pressure Vessel Code.

    Science.gov (United States)

    2010-10-01

    ... Boiler and Pressure Vessel Code. 54.01-2 Section 54.01-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND... division 1 of section VIII of the ASME Boiler and Pressure Vessel Code. (a) Pressure vessels shall be designed, constructed, and inspected in accordance with section VIII of the ASME Boiler and Pressure...

  12. The course of preexistent immune abnormalities in HIV-negative hemophiliacs treated for 2 years with a monoclonal purified factor-VIII concentrate

    NARCIS (Netherlands)

    Smid, W. M.; van der Meer, J.; Smit, J. W.; Halie, M. R.

    1993-01-01

    The administration of factor VIII concentrates has been associated with immune abnormalities in patients with severe haemophilia A, even in the absence of HIV infection. The effects of a monoclonal purified factor VIII concentrate, Hemofil M (Baxter), on preexistent immune abnormalities were

  13. Locations of All Shotpoints, USGS Cruise M1-98-GM (GOM98SHTALLG.SHP)

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — All shotpoint locations from multichannel seismics survey, USGS cruise M1-98-GM. During June 1998 and April 1999, the U.S. Geological Survey (USGS) conducted two...

  14. Flight Test Evaluation of Endurance-Maximizing Periodic Cruise Trajectories for UAV Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The benefits of periodic cruise operation of flight vehicles have been known for three decades. Although a number of papers and doctoral dissertations have studied...

  15. Oceanographic cruise Indian Ocean and Java Trench June 1969 (NODC Accession 7100908)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This report contains oceanographic data which was obtained aboard H.M.A.S DIAMANTINA during an oceanographic cruise in the Java Trench and the Indian Ocean during...

  16. Oceanographic cruise: Coral Sea, Arafura Sea, and Java Trench, April - May 1969 (NODC Accession 7100914)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This report contains oceanographic data which was obtained aboard HMAS DIAMANTINA during an oceanographic cruise in the Coral Sea, Arafura Sea, and Java Trench...

  17. Cruise NF-12-03-GRNMS (Gray's Reef National Marine Sanctuary) (EK60)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Three projects are planned for the duration of this cruise: acoustic fish tracking, marine debris surveys, and collection of A. zebra samples. Two additional...

  18. All at sea: Insights into crew work experiences on a cruise liner | Bolt ...

    African Journals Online (AJOL)

    This research explores employee experiences of working on board a cruise ship. ... jobs in the hospitality sector, the shipboard context in which employees live ... conditions and work relationships involving colleagues from diverse national ...

  19. Ichthyoplankton (biological) data collected aboard the NOAA ship Nancy Foster during cruise 0903

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Station data and ichthyoplankton (biological) data from cruise 0903 from the US Virgin Islands, British Virgin Islands, Puerto Rico, Anegada Passage, Leeward Islands...

  20. Oceanographic cruise - Coral Sea, April-May 1967 (NODC Accession 7100912)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This NODC accession contains the analog R.A.N.R.L. Technical memorandum (external) 15/69, October 1969: Oceanographic cruise - Coral Sea, April-May 1967 by P. D....

  1. Larval Fish Identification from Cruises at Oahu, TC-88-03

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — One cruise aboard the NOAA ship Townsend Cromwell was conducted during 14 April-3 May 1988. Collectors included George Boehlert, Bruce Mundy, Ronald Yoshimoto, Keith...

  2. R2R Eventlogger: Community-wide Recording of Oceanographic Cruise Science Events

    Science.gov (United States)

    Maffei, A. R.; Chandler, C. L.; Stolp, L.; Lerner, S.; Avery, J.; Thiel, T.

    2012-12-01

    Methods used by researchers to track science events during a science research cruise - and to note when and where these occur - varies widely. Handwritten notebooks, printed forms, watch-keeper logbooks, data-logging software, and customized software have all been employed. The quality of scientific results is affected by the consistency and care with which such events are recorded and integration of multi-cruise results is hampered because recording methods vary widely from cruise to cruise. The Rolling Deck to Repository (R2R) program has developed an Eventlogger system that will eventually be deployed on most vessels in the academic research fleet. It is based on the open software package called ELOG (http://midas.psi.ch/elog/) originally authored by Stefan Ritt and enhanced by our team. Lessons have been learned in its development and use on several research cruises. We have worked hard to find approaches that encourage cruise participants to use tools like the eventlogger. We examine these lessons and several eventlogger datasets from past cruises. We further describe how the R2R Science Eventlogger works in concert with the other R2R program elements to help coordinate research vessels into a coordinated mobile observing fleet. Making use of data collected on different research cruises is enabled by adopting common ways of describing science events, the science instruments employed, the data collected, etc. The use of controlled vocabularies and the practice of mapping these local vocabularies to accepted oceanographic community vocabularies helps to bind shipboard research events from different cruises into a more cohesive set of fleet-wide events that can be queried and examined in a cross-cruise manner. Examples of the use of the eventlogger during multi-cruise oceanographic research programs along with examples of resultant eventlogger data will be presented. Additionally we will highlight the importance of vocabulary use strategies to the success of the

  3. [Analysis of logistical and organizational aspects of a cruise for patients with chronic respiratory insufficiency. The RESpIRA Expedition and the COPD Cruise].

    Science.gov (United States)

    Díaz Lobato, S; Mayoralas Alises, S; Gómez Mendieta, M A; Sanz Baena, S; Martín Sánchez, R; Díaz-Agero, P

    2003-06-01

    Patients with chronic respiratory insufficiency who are receiving domiciliary oxygen therapy and mechanical ventilation report great difficulty in taking complex trips involving several destinations and prolonged stays away from home. Such patients share a common need for home equipment whose technology is relatively sophisticated, a condition that limits their freedom of movement. We are referring to systems for delivering oxygen therapy and mechanical respirators. Given that such patients have problems traveling by air, we hypothesized that a cruise would be an ideal alternative, given that travel would take place in the hotel itself. A cruise would facilitate the logistics of the journey, given that the equipment would have to be set up at only one setting. Working with these assumptions, we have thus far organized two cruises for chronic respiratory insufficiency patients: the "RESpIRA Expedition" and the "COPD Cruise". Our experience shows that the organizational problems to be coped with are patient recruitment, financing and choice of itinerary. With those aspects clear, organizers must then obtain the authorization of the cruise operator, including the approval of the medical and safety personnel on board. After obtaining permission for the cruise and as soon as the organizers know how many patients will travel, a list of oxygen therapy equipment (respirators and disposable supplies) must be compiled. Finally, equipment suppliers must be found. Afterwards, all that remains is to enjoy the trip. The participation of physicians responsible for domiciliary oxygen therapy and mechanical ventilation programs is essential for making patients feel safe and for assuring solutions for technical and medical problems that might arise.

  4. Study on a human guidance method for autonomous cruise of indoor robot

    Science.gov (United States)

    Jia, Bao-Zhi; Zhu, Ming

    2011-12-01

    This paper describes a method of human guidance for autonomous cruise of indoor robot. A low-cost robot follows a person in a room and notes the path for autonomous cruise using its monocular vision. A method of video-based object detection and tracking is taken to detect the target by the video received from the robot's camera. The validity of the human guidance method is proved by the experiment.

  5. Defense Science Board Task Force on Defense Strategies for Advanced Ballistic and Cruise Missile Threats

    Science.gov (United States)

    2017-01-01

    REPORT OF THE DEFENSE SCIENCE BOARD TASK FORCE ON Defense Strategies for Advanced Ballistic and Cruise Missile Threats January 2017 Office of... Science Board (DSB). The DSB is a Federal Advisory Committee established to provide independent advice to the Secretary of Defense. Statements, opinions... Science Board Task Force on Defense Strategies for Advanced Ballistic and Cruise Missile Threats completed its information-gathering in February 2016

  6. The scorpion toxin Amm VIII induces pain hypersensitivity through gain-of-function of TTX-sensitive Na⁺ channels.

    Science.gov (United States)

    Abbas, Najwa; Gaudioso-Tyzra, Christelle; Bonnet, Caroline; Gabriac, Mélanie; Amsalem, Muriel; Lonigro, Aurélie; Padilla, Françoise; Crest, Marcel; Martin-Eauclaire, Marie-France; Delmas, Patrick

    2013-08-01

    Voltage-gated Na(+) channels (Nav) are the targets of a variety of scorpion toxins. Here, we investigated the effects of Amm VIII, a toxin isolated from the venom of the scorpion Androctonus mauretanicus mauretanicus, on pain-related behaviours in mice. The effects of Amm VIII were compared with the classic scorpion α-toxin AaH II from Androctonus australis. Contrary to AaH II, intraplantar injection of Amm VIII at relatively high concentrations caused little nocifensive behaviours. However, Amm VIII induced rapid mechanical and thermal pain hypersensitivities. We evaluated the toxins' effects on Nav currents in nociceptive dorsal root ganglion (DRG) neurons and immortalized DRG neuron-derived F11 cells. Amm VIII and AaH II enhanced tetrodotoxin-sensitive (TTX-S) Nav currents in DRG and F11 cells. Both toxins impaired fast inactivation and negatively shifted activation. AaH II was more potent than Amm VIII at modulating TTX-S Nav currents with EC50 of 5 nM and 1 μM, respectively. AaH II and Amm VIII also impaired fast inactivation of Nav1.7, with EC50 of 6.8 nM and 1.76 μM, respectively. Neither Nav1.8 nor Nav1.9 was affected by the toxins. AaH II and Amm VIII reduced first spike latency and lowered action potential threshold. Amm VIII was less efficient than AaH II in increasing the gain of the firing frequency-stimulation relationship. In conclusion, our data show that Amm VIII, although less potent than AaH II, acts as a gating-modifier peptide reminiscent of classic α-toxins, and suggest that its hyperalgesic effects can be ascribed to gain-of-function of TTX-S Na(+) channels in nociceptors. Copyright © 2013 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.

  7. Binding of factor VIII to von willebrand factor is enabled by cleavage of the von Willebrand factor propeptide and enhanced by formation of disulfide-linked multimers.

    Science.gov (United States)

    Bendetowicz, A V; Morris, J A; Wise, R J; Gilbert, G E; Kaufman, R J

    1998-07-15

    von Willebrand factor (vWF) is a multimeric adhesive glycoprotein with one factor VIII binding site/subunit. Prior reports suggest that posttranslational modifications of vWF, including formation of N-terminal intersubunit disulfide bonds and subsequent cleavage of the propeptide, influence availability and/or affinity of factor VIII binding sites. We found that deletion of the vWF propeptide produced a dimeric vWF molecule lacking N-terminal intersubunit disulfide bonds. This molecule bound fluorescein-labeled factor VIII with sixfold lower affinity than multimeric vWF in an equilibrium flow cytometry assay (approximate KDs, 5 nmol/L v 0.9 nmol/L). Coexpression of propeptide-deleted vWF with the vWF propeptide in trans yielded multimeric vWF that displayed increased affinity for factor VIII. Insertion of an alanine residue at the N-terminus of the mature vWF subunit destroyed binding to factor VIII, indicating that the native mature N-terminus is required for factor VIII binding. The requirement for vWF propeptide cleavage was shown by (1) a point mutation of the vWF propeptide cleavage site yielding pro-vWF that was defective in factor VIII binding and (2) correlation between efficiency of intracellular propeptide cleavage and factor VIII binding. Furthermore, in a cell-free system, addition of the propeptide-cleaving enzyme PACE/furin enabled factor VIII binding in parallel with propeptide cleavage. Our results indicate that high-affinity factor VIII binding sites are located on N-terminal disulfide-linked vWF subunits from which the propeptide has been cleaved.

  8. Effect of transmembrane pressure on Factor VIII yield in ATF perfusion culture for the production of recombinant human Factor VIII co-expressed with von Willebrand factor.

    Science.gov (United States)

    Kim, Seung-Chul; An, Sora; Kim, Hyun-Ki; Park, Beom-Soo; Na, Kyu-Heum; Kim, Byung-Gee

    2016-10-01

    In this study, we evaluated three cell retention devices, an alternating tangential flow (ATF) system, a spin-filter, and a Centritech Lab III centrifuge, for the production of recombinant human Factor VIII co-expressed with von Willebrand factor. From the results, it was found that the FVIII activity in bioreactor was significantly higher in the ATF perfusion culture than two other perfusion cultures. Moreover, the FVIII activity yield was unexpectedly low in the ATF perfusion culture. We have, therefore, studied the reasons for this low FVIII activity yield. It was revealed that the inactivation and the surface adsorption of FVIII onto the harvest bag were not the main reasons for the low yield in the ATF perfusion culture. The FVIII activity yield was not increased by the use of a hollow fiber filter with 0.5 μm pore size instead of 0.2 μm pore size. Additionally, the retention of FVIII molecules by the hollow fiber filter was a dominant factor in the low FVIII activity yield in the ATF perfusion culture. We demonstrated that FVIII yield was significantly improved by controlling transmembrane pressure (TMP) across the hollow fiber filter membrane. Taken together, these results suggest that TMP control could be an efficient method for the enhancement of FVIII yield in an ATF perfusion culture.

  9. CRUISE SHIP TOURISM ON THE DANUBE RIVER. CASE STUDY: CAPITALIZATION OF DELTAIC TOURISM POTENTIAL

    Directory of Open Access Journals (Sweden)

    IRINCU Elena

    2015-12-01

    Full Text Available Over the past two decades, river cruise tourism has witnessed a strong development, being preferred by more tourists each year, to the detriment of other forms of tourism. The presence of a plethora of attractive resources, concentrated along the inland waterways represents a particular offer for tourism development, through proper planning. However, in Romania, river cruise tourism is still incipient, even though cruises on the Danube are available, on a regular basis, since the 1970s. This research focuses on cruise ship tourism on the Danube, in particularly on the deltaic sector; with the Romanian ship MS Delta Star as a case study. Following, a brief presentation of the evolution of this type of tourism on the Danube River and its peculiarities on the Romanian sector, especially in the Danube Delta, was made. The assessment framework of the tourism potential of the Danube Delta at the level of administrative-territorial units was developed by applying the methodology from the National Spatial Plan. After correlating the results of the assessment with the current capitalization of tourism potential of the delta by the cruise ship included in the study, it is highlighted the need for optimizing the structure of the offer for this tourism sector. Identifying the most valuable elements of the Danube Delta, in terms of touristical attractions and including them to future itineraries for tourists on cruise ships guarantees a better capitalization of the tourism potential attracting therefore, a greater number of tourists.

  10. Hemophilia A gene therapy via intraosseous delivery of factor VIII-lentiviral vectors.

    Science.gov (United States)

    Miao, Carol H

    2016-01-01

    Current treatment of hemophilia A (HemA) patients with repeated infusions of factor VIII (FVIII; abbreviated as F8 in constructs) is costly, inconvenient, and incompletely effective. In addition, approximately 25 % of treated patients develop anti-factor VIII immune responses. Gene therapy that can achieve long-term phenotypic correction without the complication of anti-factor VIII antibody formation is highly desired. Lentiviral vector (LV)-mediated gene transfer into hematopoietic stem cells (HSCs) results in stable integration of FVIII gene into the host genome, leading to persistent therapeutic effect. However, ex vivo HSC gene therapy requires pre-conditioning which is highly undesirable for hemophilia patients. The recently developed novel methodology of direct intraosseous (IO) delivery of LVs can efficiently transduce bone marrow cells, generating high levels of transgene expression in HSCs. IO delivery of E-F8-LV utilizing a ubiquitous EF1α promoter generated initially therapeutic levels of FVIII, however, robust anti-FVIII antibody responses ensued neutralized functional FVIII activity in the circulation. In contrast, a single IO delivery of G-FVIII-LV utilizing a megakaryocytic-specific GP1bα promoter achieved platelet-specific FVIII expression, leading to persistent, partial correction of HemA in treated animals. Most interestingly, comparable therapeutic benefit with G-F8-LV was obtained in HemA mice with pre-existing anti-FVIII inhibitors. Platelets is an ideal IO delivery vehicle since FVIII stored in α-granules of platelets is protected from high-titer anti-FVIII antibodies; and that even relatively small numbers of activated platelets that locally excrete FVIII may be sufficient to promote efficient clot formation during bleeding. Additionally, combination of pharmacological agents improved transduction of LVs and persistence of transduced cells and transgene expression. Overall, a single IO infusion of G-F8-LV can generate long-term stable

  11. Keefektifan Experiential Learning Berbantuan Origami Terhadap Kemampuan Keruangan Siswa Kelas VIII

    Directory of Open Access Journals (Sweden)

    Isnaeni Umi Machromah

    2014-11-01

    Full Text Available AbstrakPenelitian eksperimen ini bertujuan untuk mengetahui keefektifan pembelajaran Experiential Learning berbantuan origami terhadap kemampuan keruangan siswa dengan kualifikasi keefektifan yang telah ditentukan. Populasi yaitu siswa kelas VIII SMP Negeri 3 Colomadu tahun pelajaran 2012/2013. Sampel diambil secara simple random sampling. Hasil penelitian ini yaitu: (1 Uji proporsi ketuntasan belajar menunjukkan siswa kelompok eksperimen telah mencapai ketuntasan belajar; (2 Uji perbedaan dua rata-rata menghasilkan simpulan bahwa rata-rata hasil post-test kemam-puan keruangan siswa kelompok eksperimen lebih dari kelompok kontrol; (3 Analisis re-gresi menunjukkan bahwa motivasi belajar siswa memberikan pengaruh positif sebesar 49,3% terhadap kemampuan keruangan siswa; (4 Uji gain ternormalisasi menunjukkan terdapat peningkatan kemampuan keruangan siswa secara signifikan pada kategaori sedang untuk kelompok eksperimen dan kategori rendah untuk kelompok kontrol. Simpulan yang diperoleh yaitu Experiential Learning berbantuan origami efektif terhadap kemampuan keruangan siswa kelas VIII. AbstractThis experiment research aims to determine the effectiveness of Experiential Learning with Origami to the students’s spatial abilities by the criteria of effectiveness. The population of this research is students of grade VIII of SMP Negeri 3 Colomadu 2012/2013 academic year. Independent variables used in this research are learning by Experiential Learning with origami and student’s motivation. While the dependent variable is the student’s spatial abilities. This research is using documentation, spatial abilities’s test, dan motivation’s scale for collecting data method. Result of this research are: (1 proportion test show that the experiment group has reached mastering of learning clasically and personally; (2 result of spatial ablities’s post test in experiment group better than control group; (3 result of regretion shows that student

  12. Analysis of factor VIII gene inversions in 164 unrelated hemophilia A families

    Energy Technology Data Exchange (ETDEWEB)

    Vnencak-Jones, L.; Phillips, J.A. III; Janco, R.L. [Vanderbilt Univ. School of Medicine, Nashville, TN (United States)] [and others

    1994-09-01

    Hemophilia A is an X-linked recessive disease with variable phenotype and both heterogeneous and wide spread mutations in the factor VIII (F8) gene. As a result, diagnostic carrier or prenatal testing often relies upon laborious DNA linkage analysis. Recently, inversion mutations resulting from an intrachromosomal recombination between DNA sequences in one of two A genes {approximately}500 kb upstream from the F8 gene and a homologous A gene in intron 22 of the F8 gene were identified and found in 45% of severe hemophiliacs. We have analyzed banked DNA collected since 1986 from affected males or obligate carrier females representing 164 unrelated hemophilia A families. The disease was sporadic in 37%, familial in 54% and in 10% of families incomplete information was given. A unique deletion was identified in 1/164, a normal pattern was observed in 110/164 (67%), and 53/164 (32%) families had inversion mutations with 43/53 (81%) involving the distal A gene (R3 pattern) and 10/53 (19%) involving the proximal A gene (R2 pattern). While 19% of all rearrangements were R2, in 35 families with severe disease (< 1% VIII:C activity) all 16 rearrangements seen were R3. In 18 families with the R3 pattern and known activities, 16 (89%) had levels < 1%, with the remaining 2 families having {le} 2.4% activity. Further, 18 referrals specifically noted the production of inhibitors and 8/18 (45%) had the R3 pattern. Our findings demonstrate that the R3 inversion mutation patterns is (1) only seen with VIII:C activity levels of {le} 2.4%, (2) seen in 46% of families with severe hemophilia, (3) seen in 45% of hemophiliacs known to have inhibitors, (4) not correlated with sporadic or familial disease and (5) not in disequilibrium with the Bcl I or Taq I intron 18 or ST14 polymorphisms. Finally, in families positive for an inversion mutation, direct testing offers a highly accurate and less expensive alternative to DNA linkage analysis.

  13. Antidote strategies to reverse anticoagulation with TB-402, a long-acting partial inhibitor of factor VIII

    NARCIS (Netherlands)

    Tangelder, M.; Long, C.; Emmerechts, J.; Jacquemin, M.; Peerlinck, K.; Vanassche, T.; Glazer, S.; Giesen, P.; Hoylaerts, M.; Verhamme, P.

    2012-01-01

    . Background: TB-402 is a partially inhibiting antibody of factor VIII that is under development as a long-acting anticoagulant. Patients and Methods: The reversibility of FVIII inhibition by TB-402 was evaluated in vitro after spiking with recombinant human FVIII (rhFVIII), human plasma-derived FVI

  14. 77 FR 10740 - Lock+ Hydro Friends Fund VIII, FFP Project 92, LLC, Riverbank Hydro No. 24, LLC; Notice...

    Science.gov (United States)

    2012-02-23

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Lock+ Hydro Friends Fund VIII, FFP Project 92, LLC, Riverbank Hydro No. 24.... 14262-000, FFP Project 92, LLC for Project No. 14276-000, and Riverbank Hydro No. 24, LLC for Project...

  15. Evaluation of the biological differences of canine and human factor VIII in gene delivery: Implications in human hemophilia treatment

    Science.gov (United States)

    The canine is the most important large animal model for testing novel hemophilia A(HA) treatment. It is often necessary to use canine factor VIII (cFIII) gene or protein for the evaluation of HA treatment in the canine model. However, the different biological properties between cFVIII and human FVII...

  16. [Ssp DnaB intein-mediated ligation of heavy and light chains of coagulation factor VIII in Escherichia coli].

    Science.gov (United States)

    Zhu, Fuxiang; Liu, Zelong; Qu, Huige; Xin, Xiaolin; Dong, Hongxin; Liu, Xiangqin

    2009-07-01

    We studied the ligation of coagulation factor VIII heavy and light chains in Escherichia coli by utilizing the intein-mediated protein trans-splicing. A B-domain deleted factor VIII (BDD-FVIII) gene was broken into two halves of heavy and light chains before Ser1657 which meets the splicing required conserved residue and then fused to 106 and 48 amino acid-containing N-part termed Int-N and C-part termed Int-C coding sequences of split mini Ssp DnaB intein respectively. These two fusion genes were constructed into a prokaryotic expression vector pBV220. Through induction for expression of recombinant protein it displayed an obvious protein band as predicted size of BDD-FVIII protein on SDS-PAGE gel. Western blotting using factor VIII specific antibodies confirmed that this protein band is BDD-FVIII produced by protein trans-splicing. It demonstrated that the heavy and light chains of BDD-FVIII can be efficiently ligated with the Ssp DnaB intein-mediated protein trans-splicing. These results provided evidence for encouraging our ongoing investigation with intein as a means in dual AAV vectors carrying the factor VIII gene to overcome the packaging size limitation of a single AAV vector in hemophilia A gene therapy.

  17. Fifteen Questions on the Rules Regarding the Acquisition of Movables in Book VIII Draft Common Frame of Reference

    NARCIS (Netherlands)

    Salomons, A.F.

    2009-01-01

    ABSTRACT: This contribution focuses on Book VIII Draft Common Frame of Reference (DCFR) regarding acquisition and loss of ownership of movable property and consists of fifteen questions encountered when studying the rules of this book. The aim is to provide an ‘internal’ assessment of the provisions

  18. Laser rangefinders for autonomous intelligent cruise control systems

    Science.gov (United States)

    Journet, Bernard A.; Bazin, Gaelle

    1998-01-01

    THe purpose of this paper is to show to what kind of application laser range-finders can be used inside Autonomous Intelligent Cruise Control systems. Even if laser systems present good performances the safety and technical considerations are very restrictive. As the system is used in the outside, the emitted average output power must respect the rather low level of 1A class. Obstacle detection or collision avoidance require a 200 meters range. Moreover bad weather conditions, like rain or fog, ar disastrous. We have conducted measurements on laser rangefinder using different targets and at different distances. We can infer that except for cooperative targets low power laser rangefinder are not powerful enough for long distance measurement. Radars, like 77 GHz systems, are better adapted to such cases. But in case of short distances measurement, range around 10 meters, with a minimum distance around twenty centimeters, laser rangefinders are really useful with good resolution and rather low cost. Applications can have the following of white lines on the road, the target being easily cooperative, detection of vehicles in the vicinity, that means car convoy traffic control or parking assistance, the target surface being indifferent at short distances.

  19. Rapid Deployment of a RESTful Service for Oceanographic Research Cruises

    Science.gov (United States)

    Fu, Linyun; Arko, Robert; Leadbetter, Adam

    2014-05-01

    The Ocean Data Interoperability Platform (ODIP) seeks to increase data sharing across scientific domains and international boundaries, by providing a forum to harmonize diverse regional data systems. ODIP participants from the US include the Rolling Deck to Repository (R2R) program, whose mission is to capture, catalog, and describe the underway/environmental sensor data from US oceanographic research vessels and submit the data to public long-term archives. R2R publishes information online as Linked Open Data, making it widely available using Semantic Web standards. Each vessel, sensor, cruise, dataset, person, organization, funding award, log, report, etc, has a Uniform Resource Identifier (URI). Complex queries that federate results from other data providers are supported, using the SPARQL query language. To facilitate interoperability, R2R uses controlled vocabularies developed collaboratively by the science community (eg. SeaDataNet device categories) and published online by the NERC Vocabulary Server (NVS). In response to user feedback, we are developing a standard programming interface (API) and Web portal for R2R's Linked Open Data. The API provides a set of simple REST-type URLs that are translated on-the-fly into SPARQL queries, and supports common output formats (eg. JSON). We will demonstrate an implementation based on the Epimorphics Linked Data API (ELDA) open-source Java package. Our experience shows that constructing a simple portal with limited schema elements in this way can significantly reduce development time and maintenance complexity.

  20. Research combines with public outreach on a cruise ship

    Science.gov (United States)

    Williams, Elizabeth; Prager, Ellen; Wilson, Doug

    An innovative partnership among academia, government, and private industry has created a unique opportunity for oceanographic and meteorological research on a cruise ship. The University of Miami's Rosenstiel School of Marine and Atmospheric Science, Royal Caribbean International, the National Oceanic and Atmospheric Administration's Atlantic Oceanographic and Meteorological Laboratory the National Science Foundation, and the U.S. Office of Naval Research have collaborated to establish two modern laboratories for oceanic and atmospheric research on the 142,000-ton Royal Caribbean ship Explorer of the Seas.The Explorer of the Seas combines extensive research capabilities with public outreach. Hundreds of passengers experience the planet's atmosphere-ocean systems through laboratory tours and presentations given by experienced guest scientists and graduate students. In addition to weekly public lectures, guided tours of the ocean and atmospheric laboratories are available, and ocean-related films are shown during selected afternoons. Two interactive eco-learning areas onboard are equipped with a series of interactive displays and large informational touch screens that illustrate marine and atmospheric concepts as well as the onboard research program.

  1. Structural concept trends for commercial supersonic cruise aircraft design

    Science.gov (United States)

    Sakat, I. F.; Davis, G. W.; Saelman, B.

    1980-01-01

    Structural concept trends for future commercial supersonic transport aircraft are considered. Highlights, including the more important design conditions and requirements, of two studies are discussed. Knowledge of these design parameters, as determined through studies involving the application of flexible mathematical models, enabled inclusion of aeroelastic considerations in the structural-material concepts evaluation. The design trends and weight data of the previous contractual study of Mach 2.7 cruise aircraft were used as the basis for incorporating advanced materials and manufacturing approaches to the airframe for reduced weight and cost. Structural studies of design concepts employing advanced aluminum alloys, advanced composites, and advanced titanium alloy and manufacturing techniques are compared for a Mach 2.0 arrow-wing configuration concept. Appraisals of the impact of these new materials and manufacturing concepts to the airframe design are shown and compared. The research and development to validate the potential sources of weight and cost reduction identified as necessary to attain a viable advanced commercial supersonic transport are discussed.

  2. Half-life extended factor VIII for the treatment of hemophilia A.

    Science.gov (United States)

    Tiede, A

    2015-06-01

    Prophylactic infusion of factor VIII (FVIII) prevents joint bleeding and other hemorrhages in patients with hemophilia A. Conventional FVIII concentrates have a short half-life, with an average of about 12 h in adults, ranging in individual patients between 6 and 24 h, and even shorter in younger children. Therefore, effective prophylaxis requires frequent intravenous injection, usually three times per week or every other day. Several technologies are currently under investigation to extend the half-life of FVIII, including Fc fusion (Eloctate, Elocta, efmoroctocog alfa), addition of polyethylene glycol (turoctocog alfa pegol [N8-GP], BAY 94-9027, BAX 855), and a single-chain construct (CSL627). This review summarizes characteristics of products in clinical development and discusses their potential benefits.

  3. ANALISIS KESALAHAN SISWA SMP KELAS VIII DALAM MENYELESAIKAN MASALAH SOAL CERITA MATEMATIKA

    Directory of Open Access Journals (Sweden)

    Nurul Farida

    2015-12-01

    Full Text Available The aims of the research is to know mistakes and cause of mistakes mengetahui were done by students in in troubleshoot mathematics story problem. The research was a descrptive  qualitative. The subjects of the research was students of class VIII SMP Negeri 2 Karang Anyar. The result of the research are: 1 the mistakes made by students are: student incorrecting to change information that is given into mathematics expression, students can't determine formula, the students incorrectly concept, errors in interpreting the solution , do not make conclusions , errors in calculation;  2 cause students make mistakes  are : do not pay attention to a matter of intent , forget the formula , less familiar to a material , less given the questions varied, in a hurry, and less conscientious in performing the calculations Keywords: analysis, mathematics story problem, mistake

  4. Development of a peptidomimetic ligand for efficient isolation and purification of factor VIII via affinity chromatography.

    Science.gov (United States)

    Knör, Sebastian; Khrenov, Alexey V; Laufer, Burkhardt; Saenko, Evgueni L; Hauser, Charlotte A E; Kessler, Horst

    2007-09-06

    Hemophilia A, one of the most severe bleeding disorders, results from an inherited deficiency of factor VIII (FVIII) function. Treatment by injection of FVIII has been a common procedure for decades. Nevertheless, the production and purification of FVIII remains a challenging task. Current procedures using immunoaffinity chromatography are expensive and suffer from the instability of the applied antibody ligands, which elute along with the product and contaminate it. Recently, FVIII was purified by use of octapeptide ligands, but their low protease-resistance limits their application. We here report the systematic rational and combinatorial optimization procedure that allowed us to transfer the octapeptide ligands into a small peptidomimetic. This compound is the smallest ligand known for separation of such a large protein (330 kDa). It not only binds and purifies FVIII with high efficiency but also is stable, protease-resistant, and cheap to produce in preparative scale. Hence it offers a valuable alternative to antibody-based purification procedures.

  5. PENERAPAN PENDEKATAN CINEMATHERAPY UNTUK MENINGKATKAN PERILAKU PROSOSIAL PADA SISWA KELAS VIII DI BOSOWA INTERNATIONAL SCHOOL MAKASSAR

    Directory of Open Access Journals (Sweden)

    Haeruddin Niva

    2016-06-01

    Full Text Available The objectives of the research are: (i to know the prosocial behavioral level of the students in Bosowa International School Makassar before and after giving cinematography approach, and (ii to know whether or not the approach is able to enhance the prosocial behavior of the students in Bosowa International School Makassar. The research is a quantitative research using experimental method. The research design used is quasi experimental design with non-equivalent control group design. The data collection technique applied is scale and FGD (Focus Group Discussion. The findings shows that (i the prosocial behavioral level of the students was low in pretest while after giving treatment there were significant increase to the prosocial behavior in the experimental group, and (ii the cinematography approach was able to increase the prosocial behavior of the Grade VIII students in Bosowa Internatinal School Makassar. 

  6. Pharmacokinetics and dose requirements of factor VIII over the age range 3-74 years

    DEFF Research Database (Denmark)

    Björkman, Sven; Folkesson, Anna; Jönsson, Siv

    2009-01-01

    PURPOSE: The three aims of this investigation were (1) to develop a population pharmacokinetic (PK) model for factor VIII (FVIII) in haemophilia A patients, with estimates of inter-occasion and inter-individual variance, (2) to investigate whether appropriate dosing of FVIII for regular prophylaxis...... can be calculated according to patient characteristics, and (3) to present dosing recommendations for initiating prophylactic treatment. METHODS: A population PK model was developed using data from four PK studies on patients aged 7-74 years. The model was tested on sparse FVIII data from 42...... outpatient visits by haemophilia prophylaxis patients aged 3-66 years. Dose requirements for prophylaxis were calculated both according to the population model and from empirical Bayesian estimates of FVIII PK in the individual patients. RESULTS: The study data were well characterised by a two-compartment PK...

  7. Symposium by NATO Defense Research Group Panel VIII on Computer-Based Instruction in Military Environments

    CERN Document Server

    Weddle, Peter

    1987-01-01

    This collection of papers is the result of a symposium sponsored by NATO's Defense Research Group Panel VIII in the Spring of 1985. The symposium came into being when it became obvious to the NATO countries that research, development and utilization of advanced technologies for training was the best means of increasing both training effectiveness and efficiency. This symposium was the second in a series of three devoted to training. The series was structured to cover all aspects of training. The first series addressed the value of training, the second one dealt with the application of training technologies and the third and last of the series focused on academic issues concerned with the effect of prior learning on subsequent learning. The fact that a major American publisher has determined that computer based instruction is the technology of greatest interest to the NATO community is not surprising. Advances in microprocessor technology have revolutionized both how and where we train. During this symposium t...

  8. PENGARUH MODEL KOOPERATIF TIPE NHT TERHADAP HASIL BELAJAR SISWA KELAS VIII SMP NEGERI 18 MEDAN

    Directory of Open Access Journals (Sweden)

    Faridah Anum Siregar

    2012-06-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui pengaruh model Kooperatif Tipe NHT terhadap hasil belajar siswa semester genap pada sub materi pokok Tekanan Pada Zat Padat dan Zat Cair di SMP Negeri 18 Medan T.P 2009/2010. Jenis penelitian ini adalah eksperimen two group pretest postest. Populasi dalam penelitian adalah seluruh siswa kelas VIII Semester Genap SMP Negeri 18 Medan yang terdiri dari 8 kelas berjumlah 320 orang. Pengambilan sampel dilakukan dengan cara cluster random sampling yaitu dengan mengambil 2 kelas dari 8 kelas secara acak. Dari hasil uji t-test diperoleh thitung sebesar 2,458 dengan probabilitas 0,017 < 0,05. Ini berarti ada pengaruh yang signifikan penggunaan model kooperatif tipe NHT terhadap hasil belajar siswa pada sub materi pokok Tekanan Pada Zat Padat dan Zat Cair Semester Genap SMP Negeri 18 Medan T.P 2009/2010.

  9. The personality and health of King Henry VIII (1491-1547).

    Science.gov (United States)

    Keynes, Milo

    2005-08-01

    The projection of Henry VIII in the first half of his reign, which began in 1509, is of a magnificent and accomplished 'imperial prince', the possessor of superb physical health. In 1528, when aged 37, he showed a marked change in personality due, it is here argued, to depressive illness, from which he recovered by the mid-1530s. Such ill health has not been recognized previously and it engenders a need for a reassessment of his character and actions during these years of illness. He did not suffer from syphilis and the well-known leg ulcers were less incapacitating than has been described in the past. This truly enormous and overweight man was 55 years old when he died in 1547 in chronic heart failure.

  10. Assessing patients' and caregivers' perspectives on stability of factor VIII products for haemophilia A

    DEFF Research Database (Denmark)

    DiBenedetti, D B; Coles, T M; Sharma, T

    2014-01-01

    Haemophilia A is a rare inherited bleeding disorder characterized by an inability of the blood to clot normally. Patients can experience spontaneous or trauma-induced joint and soft tissue bleeding and must keep coagulation factor VIII (FVIII) accessible at all times; thus, FVIII product storage ...... convenient and accessible FVIII products for patients in daily life and while travelling. In addition, the use of social media has potential value in recruiting this population....... and stability are critical. Our primary objective was to assess haemophilia A patients' and caregivers' experiences and preferences with FVIII product storage and stability. A secondary objective was to evaluate the use of the social media site Facebook in recruitment. In this cross-sectional study, 145 English...

  11. Characterization of a Novel Alkaline Family VIII Esterase with S-Enantiomer Preference from a Compost Metagenomic Library.

    Science.gov (United States)

    Lee, Hyun Woo; Jung, Won Kyeong; Kim, Yong Ho; Ryu, Bum Han; Kim, T Doohun; Kim, Jungho; Kim, Hoon

    2016-02-01

    A novel esterase gene, est7K, was isolated from a compost metagenomic library. The gene encoded a protein of 411 amino acids and the molecular mass of the Est7K was estimated to be 44,969 Da with no signal peptide. Est7K showed the highest identity of 57% to EstA3, which is an esterase from a drinking water metagenome, when compared with the enzymes with reported properties. Est7K had three motifs, SMTK, YSV, and WGG, which correspond to the typical motifs of family VIII esterases, SxxK, Yxx, and WGG, respectively. Est7K did not have the GxSxG motif in most lipolytic enzymes. Three additional motifs, LxxxPGxxW, PLGMxDTxF, and GGxG, were found to be conserved in family VIII enzymes. The results of the phylogenetic analysis and the alignment study suggest that family VIII enzymes could be classified into two subfamilies, VIII.1 and VIII.2. The purified Est7K was optimally active at 40°C and pH 10.0. It was activated to exhibit a 2.1-fold higher activity by the presence of 30% methanol. It preferred short-length p-nitrophenyl esters, particularly p-nitrophenyl butyrate, and efficiently hydrolyzed glyceryl tributyrate. It did not hydrolyze β-lactamase substrates, tertiary alcohol esters, glyceryl trioleate, fish oil, and olive oil. Est7K preferred an Senantiomer, such as (S)-methyl-3-hydroxy-2-methylpropionate, as the substrate. The tolerance to methanol and the substrate specificity may provide potential advantage in the use of the enzyme in pharmaceutical and other biotechnological processes.

  12. Molecular Analysis of Factor VIII and Factor IX Genes in Hemophilia Patients: Identification of Novel Mutations and Molecular Dynamics Studies

    Science.gov (United States)

    Al-Allaf, Faisal A.; Taher, Mohiuddin M.; Abduljaleel, Zainularifeen; Bouazzaoui, Abdellatif; Athar, Mohammed; Bogari, Neda M.; Abalkhail, Halah A.; Owaidah, Tarek MA.

    2017-01-01

    Background Hemophilias A and B are X-linked bleeding disorders caused by mutations in the factor VIII and factor IX genes, respectively. Our objective was to identify the spectrum of mutations of the factor VIII and factor IX genes in Saudi Arabian population and determine the genotype and phenotype correlations by molecular dynamics (MD) simulation. Methods For genotyping, blood samples from Saudi Arabian patients were collected, and the genomic DNA was amplified, and then sequenced by Sanger method. For molecular simulations, we have used softwares such as CHARMM (Chemistry at Harvard Macromolecular Mechanics; http://www.charmm-gui.org) and GROMACS. In addition, the secondary structure was determined based on the solvent accessibility for the confirmation of the protein stability at the site of mutation. Results Six mutations (three novel and three known) were identified in factor VIII gene, and six mutations (one novel and five known) were identified in factor IX gene. The factor VIII novel mutations identified were c.99G>T, p. (W33C) in exon 1, c.2138 DelA, p. (N713Tfs*9) in eon14, also a novel mutation at splicing acceptor site of exon 23 c.6430 - 1G>A. In factor IX, we found a novel mutation c.855G>C, p. (E285D) in exon 8. These novel mutations were not reported in any factor VIII or factor IX databases previously. The deleterious effects of these novel mutations were confirmed by PolyPhen2 and SIFT programs. Conclusion The protein functional and structural studies and the models built in this work would be appropriate for predicting the effects of deleterious amino acid substitutions causing these genetic disorders. These findings are useful for genetic counseling in the case of consanguineous marriages which is more common in the Saudi Arabia. PMID:28270892

  13. The Arabidopsis class VIII myosin ATM2 is involved in endocytosis.

    Science.gov (United States)

    Sattarzadeh, Amirali; Franzen, Rainer; Schmelzer, Elmon

    2008-06-01

    Members of the class XI of the myosin superfamily comprising higher plant, actin-based molecular motors have been shown to be involved in peroxisome and Golgi vesicle trafficking comparable to yeast and animal class V myosins. The tasks of the second class of myosins of higher plants, class VIII, are unclear. In this study the class VIII myosin ATM2 from the model plant Arabidopsis thaliana was selected for the examination of cargo specificity in vivo. Fluorescent protein-fusion plasmid constructs with fragments of the ATM2 cDNA were generated and used for Agrobacterium tumefaciens-based transient transformation of Nicotiana benthamiana leaves. The resulting subcellular localization patterns were recorded by live imaging with confocal laser scanning microscopy (CLSM) in epidermal leaf cells. Expression of a nearly full-length construct displayed labeling of filaments and vesicles, a head + neck fragment led to decoration of filaments only. However, expression of fluorescent protein-tagged C-terminal tail domain constructs labeled vesicular structures of different appearance. Most importantly, coexpression of different RFP/YFP-ATM2 tail fusion proteins showed colocalization and, hence, binding to the same type of vesicular target. Further coexpression experiments of RFP/YFP-ATM2 tail fusion proteins with the endosomal marker FYVE and the endosomal tracer FM4-64 demonstrated colocalization with endosomes. Colocalization was also detected by expression of the CFP-tagged membrane receptor BRI1 as marker, which is constantly recycled via endosomes. Occasionally the ATM2 tail targeted to sites at the plasma membrane closely resembling the pattern obtained upon expression of the YFP-ATM1 C-terminal tail. ATM1 is known for its localization at the plasma membrane at sites of plasmodesmata. Copyright 2008 Wiley-Liss, Inc.

  14. Bioengineered coagulation factor VIII enables long-term correction of murine hemophilia A following liver-directed adeno-associated viral vector delivery

    Directory of Open Access Journals (Sweden)

    Harrison C Brown

    2014-01-01

    Full Text Available Clinical data support the feasibility and safety of adeno-associated viral (AAV vectors in gene therapy applications. Despite several clinical trials of AAV-based gene transfer for hemophilia B, a unique set of obstacles impede the development of a similar approach for hemophilia A. These include (i the size of the factor VIII (fVIII transgene, (ii humoral immune responses to fVIII, (iii inefficient biosynthesis of human fVIII, and (iv AAV vector immunity. Through bioengineering approaches, a novel fVIII molecule, designated ET3, was developed and shown to improve biosynthetic efficiency 10- to 100-fold. In this study, the utility of ET3 was assessed in the context of liver-directed, AAV-mediated gene transfer into hemophilia A mice. Due to the large size of the expression cassette, AAV-ET3 genomes packaged into viral particles as partial genome fragments. Despite this potential limitation, a single peripheral vein administration of AAV-ET3 into immune-competent hemophilia A mice resulted in correction of the fVIII deficiency at lower vector doses than previously reported for similarly oversized AAV-fVIII vectors. Therefore, ET3 appears to improve vector potency and mitigate at least one of the critical barriers to AAV-based clinical gene therapy for hemophilia A.

  15. Anti-CD3 antibodies modulate anti-factor VIII immune responses in hemophilia A mice after factor VIII plasmid-mediated gene therapy.

    Science.gov (United States)

    Peng, Baowei; Ye, Peiqing; Rawlings, David J; Ochs, Hans D; Miao, Carol H

    2009-11-12

    One major obstacle in gene therapy is the generation of immune responses directed against transgene product. Five consecutive anti-CD3 treatments concomitant with factor VIII (FVIII) plasmid injection prevented the formation of inhibitory antibodies against FVIII and achieved persistent, therapeutic levels of FVIII gene expression in treated hemophilia A mice. Repeated plasmid gene transfer is applicable in tolerized mice without eliciting immune responses. Anti-CD3 treatment significantly depleted both CD4+ and CD8+ T cells, whereas increased transforming growth factor-beta levels in plasma and the frequency of both CD4+CD25+FoxP3+ and CD4+CD25-Foxp3+ regulatory T cells in the initial few weeks after treatment. Although prior depletion of CD4+CD25+ cells did not abrogate tolerance induction, adoptive transfer of CD4+ cells from tolerized mice at 6 weeks after treatment protected recipient mice from anti-FVIII immune responses. Anti-CD3-treated mice mounted immune responses against both T-dependent and T-independent neo-antigens, indicating that anti-CD3 did not hamper the immune systems in the long term. Concomitant FVIII plasmid + anti-CD3 treatment induced long-term tolerance specific to FVIII via a mechanism involving the increase in transforming growth factor-beta levels and the generation of adaptive FVIII-specific CD4+Foxp3+ regulatory T cells at the periphery. Furthermore, anti-CD3 can reduce the titers of preexisting anti-FVIII inhibitory antibodies in hemophilia A mice.

  16. Factor VIII brand and the incidence of factor VIII inhibitors in previously untreated UK children with severe hemophilia A, 2000-2011.

    Science.gov (United States)

    Collins, Peter W; Palmer, Benedict P; Chalmers, Elizabeth A; Hart, Daniel P; Liesner, Ri; Rangarajan, Savita; Talks, Katherine; Williams, Michael; Hay, Charles R M

    2014-11-27

    The effect of recombinant factor VIII (rFVIII) brand on inhibitor development was investigated in all 407 severe hemophilia A previously untreated patients born in the United Kingdom (UK) between 1 January 2000 and 31 December 2011. Eighty-eight (22%) had been in the RODIN study. Information was extracted from the National Haemophilia Database. Because exposure days (EDs) were not known for some patients, time from first treatment was used as a surrogate for rFVIII exposure. An inhibitor developed in 118 (29%) patients, 60 high and 58 low titer, after a median (interquartile range) of 7.8 (3.3-13.5) months from first exposure and 16 (9-30) EDs. Of 128 patients treated with Kogenate Bayer/Helixate NexGen, 45 (35.2%, 95% confidence interval [CI] 27.4-43.8) developed an inhibitor compared with 42/172 (24.4%, 95% CI 18.6% to 31.4%) with Advate (P = .04). The adjusted hazard ratio (HR) (95% CI) for Kogenate Bayer/Helixate NexGen compared with Advate was 2.14 (1.12-4.10) (P = .02) for high titer and 1.75 (1.11-2.76) (P = .02) for all inhibitors. When excluding UK-RODIN patients, the adjusted HR (95% CI) for high-titer inhibitors was 2.00 (0.93-4.34) (P = .08). ReFacto AF was associated with a higher incidence of all, but not high-titer, inhibitors than Advate. These results will help inform debate around the relative immunogenicity and use of rFVIII brands.

  17. FAKTOR-FAKTOR YANG MEMPENGARUHI KUALITAS PELAYANAN PADA SCOOT FAST CRUISES DI BALI

    Directory of Open Access Journals (Sweden)

    Nengah Ardane

    2017-07-01

    Full Text Available Mode of water transport is very important in the tourism industry as a support in providing the best service for tourists. Transportation is the cause and the effect of the growth of tourist in Bali. Scoot Fast Cruises is transport services to Lembongan, Lombok and Senggigi. Based on Trip Advisor rating in the quality of service that is provided by Scoot Fast Cruises still very poor (158. This study aims to determine the factors that affect the service quality at Scoot Fast Cruises in Bali. Sampling technique used in this study using purposive sampling of respondents are crossing service users Scoot Fast Cruises in Bali with a total sample of 100 respondents. The data collection techniques using a questionnaire that was tested using the test validity and reliability. Analysis of the data used in this study is factor analysis using SPSS 17.0. The results of the factor analysis there are three factors that affect the service quality at Scoot Fast Cruises in Bali that is a factor completeness of facilities and services to get service with a value of eigen value 7.390, factor accuracy of services to the value of eigen value of 1.397 and the convenience factor rating with eigen values ??value amounting to 1.307. Factors completeness and ease of getting care facilities is a contributing factor dominant in influencing quality of tourist services at Scoot Fast Cruises in Bali. For further research that will lift the title of the research about the factors that affect the quality of service on a fast boat to take a shuttle to the hotel indicators and increasing the number of respondents and indicators. As for the company Scoot Cruises to take into account the convenience of tourists.

  18. 33 CFR 165.1324 - Safety and Security Zone; Cruise Ship Protection, Elliott Bay and Pier-91, Seattle, Washington.

    Science.gov (United States)

    2010-07-01

    ... designated as responsible for the development, implementation, revision and maintenance of the facility... such as excursion vessels, sight seeing vessels, dinner cruise vessels, and whale watching...

  19. Interpopulation study of medical attendance aboard a cruise ship.

    Science.gov (United States)

    Tomaszewski, Ryszard; Nahorski, Wacław Leszek

    2008-01-01

    The study carried out aboard a cruise ship in the years 1993-1998 involved ship passengers of various nationalities including 3872 Germans aged 23-94 years and 1281 Americans aged 25-94 years. Both nationality groups were divided into two age subgroups: till 64, and 65-94 years. The German younger age subgroup (mean age 53.2 years) consisted of 59% of the passengers, whereas the 65-94 years subgroup (mean age 72 years) was made up of 41% of the ships passengers. On the other hand, 73% of the Americans belonged to the 65-94 years subgroup (mean age 73,4 years), whereas 27% to the younger one (mean age 52.8 years). The number of onboard consultations and their causes were determined. The occurrence of chronic illnesses in both 65-94 years subgroups was assessed by means of a questionnaire. A higher frequency of consultations was found in the Germans (24.38%) than in the Americans (14.05%) (p=0.001). The difference was particularly striking in the people over 65 years of age (30.87% of the Germans as compared with 14.22% of the Americans, p=0.001). The Germans were nearly 4-times more frequently seen than the Americans for cardio-vascular diseases and almost 3-times more often because of gastrointestinal disorders. The discrepancies in the consultation rates were mainly caused by the different insurance systems of both nations. Chronic illnesses as estimated by means of the questionnaire prevailed in the German passengers. The statistically significant differences (13.3% versus 20%, p=0.01 and 0.001) regarded the locomotor system, urinary tract diseases and a group of illnesses including neurological, ophthalmological, ear, skin, malignant diseases and diabetes.

  20. Rolling Deck to Repository (R2R): Research Cruise Event Logger

    Science.gov (United States)

    Chandler, C. L.; Maffei, A. R.; Stolp, L.; Dubois, D. L.

    2011-12-01

    Scientific event logs, used by the science party to record scientific sampling events that occur during a research cruise, have been used in various ways for decades. An event log is very important for documenting the occurrence of a sampling event and its location, time, relative sequence, and related details. Event logging, done well, can provide important documentation about scientific data collected during a cruise, and enable more efficient use of those data by cruise participants and future researchers. When the resultant event log is a digital data file, with content generated programmatically and controlled by term vocabularies, it becomes an even more valuable addition to the full complement of data sets generated during the cruise. However, such a tool is only effective if it is used. Through field testing, several key factors were identified that encourage the use of such a tool: the ability to customize the tool to represent the science implementation plan, proposed sampling scheme, cruise personnel and instrumentation; the ability to retrieve date, time and location data automatically from the shipboard network and the ability to enter events quickly and from any location on the ship. This presentation provides an overview of both the current version 1 and soon to be released version 2 of the Rolling Deck to Repository (R2R) research cruise Event Logger system. The R2R Event Logger application is built upon a pre-existing, open-source, weblog product called ELOG (ELOG URL: http://midas.psi.ch/elog/). We provide an oceanographer's view of the full process by which: 1) the R2R Event Logger application is configured and tested to meet the unique needs of a research cruise; 2) the R2R project works with the ship operator to install the Event Logger on the ship designated for the cruise, 3) the Event Logger is configured to automatically retrieve location, time, and other pertinent information from the shipboard network; 4) a copy of the event log file is

  1. Schemed Power-augmented Flow for Wing-in-ground Effect Craft in Cruise

    Institute of Scientific and Technical Information of China (English)

    YANG Wei; YANG Zhigang

    2011-01-01

    To provide detailed insight into schemed power-angmented flow for wing-in-ground effect (WIG) craft in view of the concept of cruising with power assistance, this paper presents a numerical study.The engine installed before the wing for power-augmented flow is replaced by a simplified engine model in the simulations, and is considered to be equipped with a thrust vector nozzle.Flow features with different deflected nozzle angles are studied.Comparisons are made on aerodynamics to evaluate performance of power-augmented ram (PAR) modes in cruise.Considerable schemes of power-augmented flow in cruise are described.The air blown from the PAR engine accelerates the flow around wing and a high-speed attached flow near the trailing edge is recorded for certain deflected nozzle angles.This effect takes place and therefore the separation is prevented not only at the trailing edge but also on the whole upper side.The realization of suction varies with PAR modes.It is also found that scheme of blowing air under the wing for PAR engine is aerodynamically not efficient in cruise.The power-augmented flow is extremely complicated.The numerical results give clear depiction of the flow.Optimal scheme of power-augmented flow with respect to the craft in cruise depends on the specific engines and the flight regimes.

  2. A large outbreak of influenza A and B on a cruise ship causing widespread morbidity.

    Science.gov (United States)

    Brotherton, J M L; Delpech, V C; Gilbert, G L; Hatzi, S; Paraskevopoulos, P D; McAnulty, J M

    2003-04-01

    In September 2000 an outbreak of influenza-like illness was reported on a cruise ship sailing between Sydney and Noumea with over 1,100 passengers and 400 crew on board. Laboratory testing of passengers and crew indicated that both influenza A and B had been circulating on the ship. The cruise coincided with the peak influenza period in Sydney. Morbidity was high with 40 passengers hospitalized, two of whom died. A questionnaire was sent to passengers 3 weeks after the cruise and 836 of 1,119 (75%) responded. A total of 310 passengers (37%) reported suffering from an influenza-like illness (defined as cough, fever, myalgia and weakness) and 528 (63%) had seen a doctor for illness related to the cruise. One-third of passengers reported receipt of influenza vaccination in 2000; however neither their rates of influenza-like illness nor hospitalization were significantly different from those in unvaccinated passengers. A case-control study also found no significant protective effect of influenza vaccination. With the increasing popularity of cruise vacations, such outbreaks are likely to affect increasing numbers of people. Whilst influenza vaccination of passengers and crew may afford some protection, uptake and effectiveness may not be sufficient to prevent outbreaks. Surveillance systems and early intervention measures, such as antiviral therapies, should be considered to detect and control such outbreaks.

  3. PENGEMBANGAN MODEL PEMBELAJARAN BERBASIS MASALAH YANG BERMUATAN NILAI-NILAI PENDIDIKAN KARAKTER DALAM PEMBELAJARAN MENULIS CERPEN PADA KELAS VIII SMP

    Directory of Open Access Journals (Sweden)

    Aditya Dwi Haryawan

    2014-08-01

    Full Text Available Menulis merupakan suatu keterampilan yang harus dimiliki peserta didik sekolah menengah pertama. Melaluitulisan, peserta didik dapat menyampaikan pengalaman, pikiran, perasaan, atau keinginan. Masalah penelitianini adalah (1 bagaimanakah kebutuhan pengembangan model pembelajaran berbasis masalah yang bermuatannilai-nilai pendidikan karakter dalam pembelajaran menulis cerpen berdasarkan persepsi guru dan pesertadidik kelas VIII SMP?; (2 bagaimanakah prinsip-prinsip pengembangan model pembelajaran berbasis masalahyang bermuatan nilai-nilai pendidikan karakter dalam pembelajaran menulis cerpen pada peserta didik kelasVIII SMP? (3 bagaimanakah pengembangan prototipe model pembelajaran berbasis masalah yang bermuatannilai-nilai pendidikan karakter dalam pembelajaran menulis cerpen pada kelas VIII SMP?; (4 bagaimanakahkeberterimaan penerapan pengembangan model pembelajaran berbasis masalah yang bermuatan nilai-nilaipendidikan karakter dalam pembelajaran menulis cerpen pada kelas VIII SMP. Dalam penelitian ini digunakandesain Research and Development. Tahapan yang dilakukan dalam penelitian ini adalah studi pustaka, analisiskebutuhan, pengembangan model yang dikembangkan, uji validasi, revisi, implementasi, revisi akhir,pengembangan model/produk akhir. Pengumpulan data dilakukan dengan observasi, angket, wawancara, tes,dan dokumentasi foto. Writing is a skill that must be owned by middle school students. Through writing, students canconvey experiences, thoughts, feelings, or desires. The research problem is (1 How can the needsof the development of PBL model that charged the values of character education in learning towrite short stories on the perceptions of teachers and students of class VIII?; (2 How do thecharacteristics of the development of PBL model value-laden -the value of character education inlearning to write short stories at the high school of learner assessment system class?; (3 How isthe development of a prototype model of PBL  charged

  4. Defect-free zinc-blende structured InAs nanowires realized by in situ two V/III ratio growth in molecular beam epitaxy.

    Science.gov (United States)

    Zhang, Zhi; Lu, Zhen-Yu; Chen, Ping-Ping; Lu, Wei; Zou, Jin

    2015-08-07

    In this study, we devised a two-V/III-ratio procedure to control the Au-assisted growth of defect-free InAs nanowires in molecular beam epitaxy. The demonstrated two V/III ratio procedure consists of a first high V/III ratio growth step to prepare the nanowire foundation on the substrate surface, followed by a low V/III ratio step to induce the nanowire growth. By manipulating the V/III ratios in different steps, we have achieved the controlled growth of pure defect-free zinc-blende structured InAs nanowires on the GaAs {1̄1̄1̄} substrates. This study provides an approach to control not only the crystal structure of semiconductor nanowires, but also their structural qualities.

  5. 20 CFR 408.931 - How much will we withhold from your title II and title XVI benefits to recover a title VIII...

    Science.gov (United States)

    2010-04-01

    ... willfully misrepresented or concealed material information in connection with the overpayment. (c) In... withholding from title VIII benefits. (d) If you or your spouse willfully misrepresented or concealed material...

  6. ORGANIZATION AND IMPLEMENTATION OF INTEGRATED MANAGEMENT SYSTEM PROCESSES - CRUISE PORT DUBROVNIK

    Directory of Open Access Journals (Sweden)

    Ivona Vrdoljak Raguz

    2012-01-01

    Full Text Available World cruise market is very dynamic and it is characterised by constant changes in offer and demand. Dubrovnik, as one of the leading port in the Mediterranean is faced with the problem of large concentrations of ships and passengers in a short period of time. Paper provides answers to the questions: how to manage cruise tourism in Dubrovnik? What are the guidelines for the further development of cruising in Dubrovnik? Modern ports management system must be organized and managed in a manner that will ensure the recognition requirements of stakeholders and their fulfilment. All this requires a more complex integrated management system, in which the requirements of quality management will be the basis, and requirements of environmental management needed an upgrade.

  7. Library holdings for EX0901: Mapping Shakedown Cruise on NOAA Ship Okeanos Explorer between March 29, 2009 and April 3, 2009

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Library Catalog may include: Data Management Plans, Cruise Plans, Cruise Summary Reports, Scientific "Quick Look Reports", Video Annotation Logs, Image Collections,...

  8. Library holdings for EX0902: Mapping Shakedown Cruise on NOAA Ship Okeanos Explorer between April 25, 2009 and April 30, 2009

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Library Catalog may include: Data Management Plans, Cruise Plans, Cruise Summary Reports, Scientific "Quick Look Reports", Video Annotation Logs, Image Collections,...

  9. Tecniche costruttive e forme di potere nella Toscana sud-occidentale (secc. VIII-XIV

    Directory of Open Access Journals (Sweden)

    Bianchi, Giovanna

    2005-12-01

    Full Text Available This paper tells about the analysis of the building techniques of elevations inferred from data obtained in extensive projects of archaeological research executed in western Tuscany's rural field from half '90s of last century to present. The text looks over the changes from wooden building trade related to the first high rise habitants in VIIth-VIIIth century, until the pattern settlements in the second half of VIIIth and XIth centuries, characterized by the first use of masonry and the presence of expert master builders. The more complex organization of the building workshops for castles of the XIth and XIIth centuries in relation with the liege lord's rising politic abusive authority is explained in the following part. The subsequent formation of new suburbs between the XIIIth and the XIV centuries is characterized by a different way of use of building techniques, often founded over pre-existing castles, linked to local council, up to the political and economical influence of Pisa in this territory.Nell’articolo si tratta l’analisi delle tecniche murarie desunta da dati provenienti da ampi progetti di indagine archeologica svolti in ambito rurale nella Toscana occidentale dalla metà degli anni Novanta dello scorso secolo ad oggi. Nel testo si esaminano i cambiamenti dei modi di edificare a partire dall’edilizia in legno dei primi abitati di altura di VII-VIII secolo, sino agli insediamenti più strutturati di seconda metà VIII e IX secolo, caratterizzati da un primo uso della pietra e dalla presenza di maestranze specializzate. In seguito si analizza la più complessa organizzazione del cantiere propria della costruzione dei castelli di XI e XII secolo in rapporto ai poteri politici ed economici delle nascenti signorie territoriali. Un differente uso delle tecniche costruttive caratterizza la successiva formazione di nuovi borghi tra XIII e XIV secolo, spesso impiantati sui preesistenti castelli, legati ai locali organismi comunali

  10. Potential role of a new PEGylated recombinant factor VIII for hemophilia A

    Directory of Open Access Journals (Sweden)

    Wynn TT

    2016-06-01

    Full Text Available Tung Thanh Wynn,1 Burak Gumuscu,2,3 1Department of Pediatrics, Division of Pediatric Hematology/Oncology, University of Florida, Gainesville, FL, 2Pediatric Hematology-Oncology, Bon Secours Health System, St. Mary’s Hospital, Richmond, VA, 3Department of Pediatrics, Division of Pediatric Hematology/Oncology, University of Virginia, Charlottesville, VA, USA Abstract: Hemophilia A, a deficiency in the activity of coagulation factor (F VIII, is an X-linked bleeding disorder with an approximate incidence of one in 5,000 male infants. Bleeding-related complications often result in greater severity of disease, poor quality of life, surgical interventions for severe joint destruction, and shortened life span. With the availability of plasma-derived and recombinant FVIII products, the benefits of primary prophylaxis were demonstrated and is now the standard of care for patients with severe factor deficiencies. Current hemophilia research is focusing on the creation of new factor replacement therapies with longer half-lives; accessing alternative mechanisms to achieve desired hemostasis and enhance bypassing ­activity; and limiting the immunogenicity of the protein. PEGylation involves the covalent attachment of polyethylene glycol (PEG to a protein, peptide, or a small molecule drug. PEG effectively increases the molecular weight and size of the protein by creating a hydrophilic cloud around the molecule. This molecular change may reduce susceptibility of the molecule to proteolytic activity and degradation. It is also believed that PEGylation changes the surface charge of the protein that ultimately interferes with some receptor-mediated clearance processes. The half-life of PEGylated factor is more prolonged when compared to non-PEGylated full-length recombinant FVIII. The dawn of a new era in the care of hemophilia patients is upon us with the release of recombinant FVIII products with extended half-lives, and products with even more extended half

  11. Kubas-type hydrogen storage in V(III) polymers using tri- and tetradentate bridging ligands.

    Science.gov (United States)

    Hoang, Tuan K A; Hamaed, Ahmad; Moula, Golam; Aroca, Ricardo; Trudeau, Michel; Antonelli, David M

    2011-04-01

    Oxalic acid, oxamide, glycolic acid, and glycolamide were employed as 2-carbon linkers to synthesize a series of one-dimensional V(III) polymers from trismesityl vanadium(III)·THF containing a high concentration of low-valent metal sites that can be exploited for Kubas binding in hydrogen storage. Synthesized materials were characterized by powder X-ray diffraction (PXRD), nitrogen adsorption (BET), X-ray photoelectron spectroscopy (XPS), infrared spectroscopy (IR), Raman spectroscopy, thermogravimetric analysis, and elemental analysis. Because each of these organic linkers possesses a different number of protons and coordinating atoms, the products in each case were expected to have different stoichiometries with respect to the number of mesityl groups eliminated and also a different geometry about the V(III) centers. For example, the oxalate and glycolate polymers contained residual mesityl groups; however, these could be exchanged with hydride via hydrogenolysis. The highest adsorption capacity was recorded on the product of trismesityl vanadium(III)·THF with oxamide (3.49 wt % at 77 K and 85 bar). As suggested by the high enthalpy of adsorption (17.9 kJ/mol H(2)), a substantial degree of performance of the vanadium metal centers was retained at room temperature (25%), corresponding to a gravimetric adsorption of 0.87 wt % at 85 bar, close to the performance of MOF-177 at this temperature and pressure. This is remarkable given the BET surface area of this material is only 9 m(2)/g. A calculation on the basis of thermogravimetric results provides 0.88 hydrogen molecule per vanadium center under these conditions. Raman studies with H(2) and D(2) showed the first unequivocal evidence for Kubas binding on a framework metal in an extended solid, and IR studies demonstrated H(D) exchange of the vanadium hydride with coordinated D(2). These spectroscopic observations are sufficient to assign the rising trends in isosteric heats of hydrogen adsorption observed

  12. Elevated factor VIII level and stroke in patients without traditional risk factors associated with cardiovascular diseases

    Directory of Open Access Journals (Sweden)

    Lasek-Bal A

    2013-06-01

    Full Text Available Anetta Lasek-Bal, Przemyslaw Puz, Zofia KazibutowskaStroke Unit, Department of Neurology, Medical University of Silesia, Professor Leszek Giec Upper Silesian Medical Centre, Katowice, PolandIntroduction: Hemostasis is affected by interactions between physiological processes, including those connected with the coagulation system, whose essence is converting fibrinogen into fibrin. The role of factor VIII (FVIII consists in activating factor X, which directly participates in the generation of thrombin, which is able to produce stable fibrin, which in turn forms blood clots. There are divergent opinions regarding the significance of high levels of FVIII in stroke pathogenesis.Aim: The aim of our study was to evaluate FVIII activity in individuals with cryptogenic stroke in order to determine a potential relationship between it and cerebral ischemia.Material and methods: Nine patients suffering with stroke were used in this study: six women and three men aged 49–63 years. In all of the patients, the presence of known and potential risk factors for stroke had been excluded during previous diagnostic procedures. These patients accounted for 1.2% of the 719 people who suffered a stroke and were hospitalized in 2011 at the Stroke Unit. FVIII activity was examined in each of the nine qualified subjects within 1–2 months of the occurrence of stroke (the first test and repeated (the second test in five patients with abnormal results obtained from the first examination.Results: Increased activity of FVIII was found in 5 out of 9 patients. In patients with abnormal results, elevated FVIII was found in follow-up examinations in the 8th–10th month following stroke. Hemodynamic abnormalities in carotid or cerebral artery (presence of thrombus were found in 3 of the 5 patients with increased FVIII levels. In the first 24 hours following stroke the neurological state of patients with abnormal FVIII was worse than individuals with normal FVIII activity. The

  13. 78 FR 10172 - Lisa Anne Cornell and G. Ware Cornell, Jr. v. Princess Cruise Lines, Ltd. (Corp), Carnival PLC...

    Science.gov (United States)

    2013-02-13

    ... Lisa Anne Cornell and G. Ware Cornell, Jr. v. Princess Cruise Lines, Ltd. (Corp), Carnival PLC, and..., Jr., hereinafter ``Complainants,'' against Princess Cruise Lines, Ltd (Corp), Carnival plc, and... common carrier for hire of passengers from ports in the United States;'' Respondent Carnival plc ``is...

  14. IMPLEMENTASI MODEL PEMBELAJARAN ARIAS DALAM UPAYA MENINGKATKAN PARTISIPASI DAN HASIL BELAJAR EKONOMI SISWA KELAS VIII PADA SMP N 10 PADANG

    Directory of Open Access Journals (Sweden)

    nilma desri rosya

    2015-10-01

    Full Text Available This study aims to see increased participation and student learning outcomes by using model study on subjects ARIAS Economy class VIII.3 in SMP N 10 Padang. This research is a classroom action research with the hypothesis of action is the application of learning models ARIAS can increase participation and learning outcomes Economy class students VIII.3 SMP N 10 Padang. Data were collected through observation sheet that is used to view changes in participation and learning outcomes of students in the learning process cycle I and II. The results of the two cycles shows that participation and learning outcomes of students has increased since the first cycle and this improvement continued in the second cycle

  15. V/III ratio effects on high quality InAlAs for quantum cascade laser structures

    Science.gov (United States)

    Demir, Ilkay; Elagoz, Sezai

    2017-04-01

    In this study we report the V/III ratio effects on growth, structural, optical and doping characteristics of low growth rate (∼1 Å/s) heteroepitaxial Metal Organic Chemical Vapor Deposition (MOCVD) grown InxAl1-xAs layers, a part of Quantum Cascade Laser (QCL) structures, on InP substrate. Especially photoluminescence (PL) properties of InAlAs-InP interface show strong dependence on AsH3 overpressure. We have shown that the V/III ratio with fixed metalorganic precursor flow is a crucial parameter on InxAl1-xAs layers to have a good material quality in terms of crystallinity, optical and electrical characteristics with and without doping.

  16. PENGARUH KEMANDIRIAN BELAJAR DAN PENGGUNAAN UANG SAKU TERHADAP PRESTASI BELAJAR KOMPETENSI DASAR EKONOMI KELAS VII DAN VIII

    Directory of Open Access Journals (Sweden)

    Apri Dwi Astuti

    2012-10-01

    Full Text Available Kemandirian belajar membantu siswa untuk bertanggung jawab dan menumbuhkan kesadaran belajar. Penggunaan uang saku menumbuhkan jiwa mandiri, jujur dan komitmen. Adapun hasil observasi awal menunjukkan masih ada siswa kels VII dan VIII SMP Semesta untuk kompetensi dasar ekonomi dibawah KKM. Tujuan penelitian ini untuk mengetahui pengaruh kemandirian belajar dn penggunaan uang saku terhadap prestasi belajar kompetensi dasar ekonomi kelas VII dan VIII SMP Semesta. Populasi penelitian ini adalah semua siswa kelas VII dan VIII SMP Semesta Boarding School Semarang. Variabel penelitian ini adalah kemandirian belajar dan penggunaan uang saku sebagai variabel bebas, sedangkan untuk� variabel terikat adalah prestasi belajar kompetensi dasar ekonomi. Data diambil dengan metode wawancara, kuesioner dan dokumentasi guru. Analisis data menggunakan analisis regresi linear berganda.Berdasarkan penelitian secara simultan menunjukkan adanya pengaruh antara kemandirian belajar dan penggunaan uang saku terhadap prestasi belajar kompetensi dasar ekonomi sebesar 51,3%. Secara parsial, pengaruh kemandirian belajar terhadap prestasi belajar kompetensi dasar ekonomi sebesar 66,2% dan pengaruh penggunaan uang saku terhadap prestasi belajar kompetensi dasar ekonomi sebesar 62,5%.Berdasarkan hasil penelitian diatas disimpulkan bahwa kemandirian belajar dan penggunaan uang saku berpengaruh positif baik secara simultan maupun parsial terhadap prestasi belajar kompetensi dasar ekonomi kelas VII dan VIII SMP Semesta Boarding school semarang tahun pelajaran 2011/2012. Saran yang berkaitan dengan penelitian ini yaitu : 1 Sekolah disarankan untuk memberikan batasan siswa pulang ke rumah agar dapat membantu siswa beradaptasi dengan cepat dan mudah tinggal di asrama. 2 Orang tua disarankan untuk memberikan uang saku yang disesuaikan dengan kebutuhan siswa. 3 Siswa disarankan untuk meningkatkan kemandirian dalam aspek belajar terutama bagi mata pelajaran yang masih kurang dari

  17. Recombinant B domain deleted porcine factor VIII for the treatment of bleeding episodes in adults with acquired hemophilia A.

    Science.gov (United States)

    Gomperts, Edward

    2015-08-01

    Hemophilia A is an inherited deficiency of clotting factor VIII (FVIII) often complicated by inhibitor development (CHAWI) in which neutralizing antibodies block the therapeutic benefit of replacement therapy. Inhibitors to FVIII can also be seen in an auto-immune disease known as acquired hemophilia A (AHA). 'Bypassing' therapies have been shown to provide hemostasis but dosing must be done empirically because current assays cannot measure objective markers of treatment efficacy and safety. A recombinant porcine sequence factor VIII (r-pFVIII) has been developed for the management of AHA. Preclinical, Phase I and Phase II clinical research studies in CHAWI subjects showed therapeutic potential and safety of this agent. A Phase II/III study in AHA with serious bleeding episodes shows a positive response in all subjects after administration. Based on current preclinical and clinical trial data, r-pFVIII should become the first line of treatment in the management of hemorrhage in patients with AHA.

  18. Structure and stability of hexa-aqua V(III) cations in vanadium redox flow battery electrolytes.

    Science.gov (United States)

    Vijayakumar, M; Li, Liyu; Nie, Zimin; Yang, Zhenguo; Hu, JianZhi

    2012-08-01

    The vanadium(III) cation structure in mixed acid based electrolyte solution from vanadium redox flow batteries is studied by (17)O and (35/37)Cl nuclear magnetic resonance (NMR) spectroscopy, electronic spectroscopy and density functional theory (DFT) based computational modelling. Both computational and experimental results reveal that the V(III) species can complex with counter anions (sulfate/chlorine) depending on the composition of its solvation sphere. By analyzing the powder precipitate it was found that the formation of sulfate complexed V(III) species is the crucial process in the precipitation reaction. The precipitation occurs through nucleation of neutral species formed through deprotonation and ion-pair formation process. However, the powder precipitate shows a multiphase nature which warrants multiple reaction pathways for precipitation reaction.

  19. Efficacy and safety of rVIII-SingleChain: results of a phase 1/3 multicenter clinical trial in severe hemophilia A

    Science.gov (United States)

    Mahlangu, Johnny; Kuliczkowski, Kazimierz; Karim, Faraizah Abdul; Stasyshyn, Oleksandra; Kosinova, Marina V.; Lepatan, Lynda Mae; Skotnicki, Aleksander; Boggio, Lisa N.; Klamroth, Robert; Oldenburg, Johannes; Hellmann, Andrzej; Santagostino, Elena; Baker, Ross I.; Fischer, Kathelijn; Gill, Joan C.; P’Ng, Stephanie; Chowdary, Pratima; Escobar, Miguel A.; Khayat, Claudia Djambas; Rusen, Luminita; Bensen-Kennedy, Debra; Blackman, Nicole; Limsakun, Tharin; Veldman, Alex; St. Ledger, Katie

    2016-01-01

    Recombinant VIII (rVIII)-SingleChain is a novel B-domain–truncated recombinant factor VIII (rFVIII), comprised of covalently bonded factor VIII (FVIII) heavy and light chains. It was designed to have a higher binding affinity for von Willebrand factor (VWF). This phase 1/3 study investigated the efficacy and safety of rVIII-SingleChain in the treatment of bleeding episodes, routine prophylaxis, and surgical prophylaxis. Participants were ≥12 years of age, with severe hemophilia A (endogenous FVIII <1%). The participants were allocated by the investigator to receive rVIII-SingleChain in either an on-demand or prophylaxis regimen. Of the 175 patients meeting study eligibility criteria, 173 were treated with rVIII-SingleChain, prophylactically (N = 146) or on-demand (N = 27). The total cumulative exposure was 14 306 exposure days (EDs), with 120 participants reaching ≥50 EDs and 52 participants having ≥100 EDs. Hemostatic efficacy was rated by the investigator as excellent or good in 93.8% of the 835 bleeds treated and assessed. Across all prophylaxis regimens, the median annualized spontaneous bleeding rate was 0.00 (Q1, Q3: 0.0, 2.4) and the median overall annualized bleeding rate (ABR) was 1.14 (Q1, Q3: 0.0, 4.2). Surgical hemostasis was rated as excellent/good in 100% of major surgeries by the investigator. No participant developed FVIII inhibitors. In conclusion, rVIII-SingleChain is a novel rFVIII molecule showing excellent hemostatic efficacy in surgery and in the control of bleeding events, low ABR in patients on prophylaxis, and a favorable safety profile in this large clinical study. This trial was registered at www.clinicaltrials.gov as #NCT01486927. PMID:27330001

  20. Efficacy and safety of rVIII-SingleChain: results of a phase 1/3 multicenter clinical trial in severe hemophilia A.

    Science.gov (United States)

    Mahlangu, Johnny; Kuliczkowski, Kazimierz; Karim, Faraizah Abdul; Stasyshyn, Oleksandra; Kosinova, Marina V; Lepatan, Lynda Mae; Skotnicki, Aleksander; Boggio, Lisa N; Klamroth, Robert; Oldenburg, Johannes; Hellmann, Andrzej; Santagostino, Elena; Baker, Ross I; Fischer, Kathelijn; Gill, Joan C; P'Ng, Stephanie; Chowdary, Pratima; Escobar, Miguel A; Khayat, Claudia Djambas; Rusen, Luminita; Bensen-Kennedy, Debra; Blackman, Nicole; Limsakun, Tharin; Veldman, Alex; St Ledger, Katie; Pabinger, Ingrid

    2016-08-04

    Recombinant VIII (rVIII)-SingleChain is a novel B-domain-truncated recombinant factor VIII (rFVIII), comprised of covalently bonded factor VIII (FVIII) heavy and light chains. It was designed to have a higher binding affinity for von Willebrand factor (VWF). This phase 1/3 study investigated the efficacy and safety of rVIII-SingleChain in the treatment of bleeding episodes, routine prophylaxis, and surgical prophylaxis. Participants were ≥12 years of age, with severe hemophilia A (endogenous FVIII <1%). The participants were allocated by the investigator to receive rVIII-SingleChain in either an on-demand or prophylaxis regimen. Of the 175 patients meeting study eligibility criteria, 173 were treated with rVIII-SingleChain, prophylactically (N = 146) or on-demand (N = 27). The total cumulative exposure was 14 306 exposure days (EDs), with 120 participants reaching ≥50 EDs and 52 participants having ≥100 EDs. Hemostatic efficacy was rated by the investigator as excellent or good in 93.8% of the 835 bleeds treated and assessed. Across all prophylaxis regimens, the median annualized spontaneous bleeding rate was 0.00 (Q1, Q3: 0.0, 2.4) and the median overall annualized bleeding rate (ABR) was 1.14 (Q1, Q3: 0.0, 4.2). Surgical hemostasis was rated as excellent/good in 100% of major surgeries by the investigator. No participant developed FVIII inhibitors. In conclusion, rVIII-SingleChain is a novel rFVIII molecule showing excellent hemostatic efficacy in surgery and in the control of bleeding events, low ABR in patients on prophylaxis, and a favorable safety profile in this large clinical study. This trial was registered at www.clinicaltrials.gov as #NCT01486927.

  1. Recent cruise onboard R/V Sonne to the Carlsberg Ridge and the Andaman Sea

    Digital Repository Service at National Institute of Oceanography (India)

    KameshRaju, K.A.

    analysis was carried out for pH, dissolved oxygen, and sio4 . Water samples were collected in specially designed copper tubes for the analysis of helium . Many of the results from this cruise were reported at a special session at the aoGs 2008 meeting...R) and the andaman backarc spreading center (absc; fig . 1) . The cruise was conducted onboard the German research vessel R/V Sonne (fig . 2) chartered by the national Institute of oceanography (nIo) . We also collaborated with the Hawaii Mapping Research Group...

  2. Multiobjective Design Optimization of Supersonic Jet Engine in Different Cruise Mach Numbers

    Science.gov (United States)

    Ogawa, Masamichi; Sato, Tetsuya; Kobayashi, Hiroaki; Taguchi, Hideyuki

    The aim of this paper is to apply a multi-objective optimization generic algorithm (MOGA) to the conceptual design of the hypersonic/supersonic vehicles with different cruise Mach number. The pre-cooled turbojet engine is employed as a propulsion system and some engine parameters such as the precooler size, compressor size, compression ratio and fuel type are varied in the analysis. The result shows that the optimum cruise Mach number is about 4 if hydrogen fuel is used. Methane fuel instead of hydrogen reduces the vehicle gross weight by 33% in case of the Mach 2 vehicle.

  3. Outbreak of Legionnaires' disease on a cruise ship: lessons for international surveillance and control.

    Science.gov (United States)

    Regan, C M; McCann, B; Syed, Q; Christie, P; Joseph, C; Colligan, J; McGaffin, A

    2003-06-01

    A sporadic case of Legionnaires' disease was linked to travel on a cruise ship. Investigation identified two further cases of Legionnaires' Disease and one case of non-pneumonic Legionella infection. An Incident Team confirmed the source to be the ship's water system and control measures were instituted that included pasteurisation, super chlorination and chlorine dioxide dosing. The Public Health Laboratory Service (PHLS) Communicable Disease Surveillance Centre (CDSC), through the European Surveillance Scheme for Travel Associated Legionnaires' Disease, identified three previous cases associated with the same ship's water system including one fatality. Lessons for the international surveillance and control of Legionnaires' disease on cruise ships are discussed.

  4. MX Siting Investigation. Geotechnical Evaluation. Volume VIII. Nevada - Utah Verification Studies, FY 79. Geotechnical Data Big Smoky CDP, Nevada.

    Science.gov (United States)

    1979-08-24

    Angular - particles have sharp edges and relatively plane sides with unpolished surfaces. wfl . AT#UNL, EWE. FN-TR-27-VIII 6-5 Subangular - particles... GEM ~GIC UNIT My ’All .’j GP T-3 C-33 St FACE ELEV TION: 492j * 1500m) SM jSIRFICIAL GE LOGIC UNIT: AMy A3 SW-SM F -6 C-34 SURFACE ELEVATION: 4938

  5. Neurotology findings in patients with diagnosis of vascular loop of cranial nerves VIII in magnetic resonance imaging

    Directory of Open Access Journals (Sweden)

    Grocoske, Francisco Luiz Busato

    Full Text Available Introduction: The vascular compression by redundant vessels under the VIII cranial nerves has been studied since the 80's, and many authors proposed correlations between the compression and the otoneurological findings (vertigo, tinnitus, hypoacusis, audiometry and electrophysiological findings. Objective: Analyze and correlate the different signs and otoneurological symptoms, the audiological findings and its incidence over individuals with Vascular Loop (VL diagnosis of VIII cranial nerves by magnetic resonance imaging (MRI. Method: Retrospective study through the analysis of medical records of 47 patients attended in the otoneurology clinic of Clinical Hospital of UFPR. All the patients have MRI exams with compatible pictures of VL of the VIII cranial nerves. Results: The tinnitus was the most frequent symptom, in 83% of the patients, followed by hypoacusis (60% and vertigo (36%. The audiometry presented alterations in 89%, the brainstem evoked auditory potential in 33% and the vecto-electronystagmography in 17% of the patients. Was not found statistically significant relation between the buzz or hypoacusis, and the presence of VL in MRI. Only 36% of patients had complaints of vertigo, the main symptom described in theory of vascular compression of the VIII pair of nerve. As in the audiometry and in brainstem evoked auditory potential was not found a statistically significant relation between the exam and the presence of the VL in the RMI. Conclusion: The results show independence between the findings of the RMI, clinical picture and audiological results (p>0,05 suggesting that there are no exclusive and direct relation between the diagnosis of vascular loop in the MRI and the clinical picture matching.

  6. Development of Integrated Natural Science Teaching Materials Webbed Type with Applying Discourse Analysis on Students Grade VIII in Physics Class

    Science.gov (United States)

    Sukariasih, Luh

    2017-05-01

    This study aims to produce teaching materials integrated natural science (IPA) webbed type of handout types are eligible for use in integrated science teaching. This type of research IS a kind of research and development / Research and Development (R & D) with reference to the 4D development model that is (define, design, develop, and disseminate). Data analysis techniques used to process data from the results of the assessment by the validator expert, and the results of the assessment by teachers and learners while testing is limited (12 students of class VIII SMPN 10 Kendari) using quantitative descriptive data analysis techniques disclosed in the distribution of scores on the scale of five categories grading scale that has been determined. The results of due diligence material gain votes validator material in the category of “very good” and “good”, of the data generated in the feasibility test presentation obtained the category of “good” and “excellent”, from the data generated in the feasibility of graphic test obtained the category of “very good “and” good “, as well as of the data generated in the test the feasibility of using words and language obtained the category of“very good “and” good “, so with qualifications gained the teaching materials IPA integrated type webbed by applying discourse analysis on the theme of energy and food for Junior High School (SMP) grade VIII suitable as teaching materials. In limited testing, data generated in response to a science teacher at SMPN 10 Kendari to product instructional materials as “excellent”, and from the data generated while testing is limited by the 12 students of class VIII SMPN 10 Kendari are more students who score indicates category “very good”, so that the qualification obtained by the natural science (IPA) teaching material integrated type webbed by applying discourse analysis on the theme of energy and food for SMP / class VIII fit for use as teaching material.

  7. The Funnel Geometry of Open Flux Tubes in the Low Solar Corona Constrained by O VI and Ne VIII Outflow

    Science.gov (United States)

    Byhring, Hanne S.; Esser, Ruth; Lie-Svendsen, Oystein

    2008-01-01

    Model calculations show that observed outflow velocities of order 7-10 km/s of C IV and O VI ions, and 15-20 km/s of Ne VIII ions, are not only consistent with models of the solar wind from coronas holes, but also place unique constraints on the degree of flow tube expansion as well as the location of the expansion in the transition region/lower corona.

  8. MENINGKATKAN PEMBELAJARAN HOCKEY MELALUI PERMAINAN 4 GAWANG KECIL PADA SISWA KELAS VIII 3 SMP MUHAMMADIYAH 4 SEMARANG TAHUN 2015

    Directory of Open Access Journals (Sweden)

    Muhammad Taufiq

    2016-02-01

    Full Text Available The purpose of this result the study learning of hockey games through the 4 Small Wicket Game in Grade VIII 3 students of Smp Muhammadiyah 4 Semarang. In this case the right is 4 semarang. research method. In practice consists of two cycles. Based on the results of the research, it showed the percentage of passing students study result in the first cycle, students who got pass score are 58.33% (21 students and who did not pass are 41.66% (15 students. In the second cycle students who got pass score are 88.88% (34 students and students who did not pass are 11.11% (4 students. It indicates an increase completeness of student study result is 30.55%.Based on the research’s result, it can be concluded that the use of 4 Small Wicket Game in Grade VIII 3 students is right to improve the study result of grade VIII 3 students of SMP Muhammadiyah 4 Semarang in learning hockey.

  9. In vitro differentiation of mouse ES cells into hepatocytes with coagulation factors VIII and IX expression profiles

    Institute of Scientific and Technical Information of China (English)

    MENG; Ying; HUANG; Shaoliang; MIN; Jun; GUO; Zhongmin

    2006-01-01

    Coagulation factors II, V, VII, VIII, IX and X are produced by hepatocytes. So factors VIII and IX deficiencies, which result in hemophilia A and B, have the potential to respond to cellular re- placement therapy. Embryonic stem (ES) cells provide a unique source for therapeutic applications. Here, E14 mouse ES cells have been induced into hepatocytes in vitro. Morphology revealed that ES-derived hepatic-like cells were round or polyhedral shaped with distinct boundary of individual cells, and some arranged in trabeculae. These cells expressed endodermal- or liver-specific mRNA --transthyretin (TTR), α1-anti-trypsin (AAT), α-fetoprotein (AFP), albumin (ALB), glucose-6- phoshpatase (G6P) and tyrosine aminotransferase (TAT). Approximately (85.1(0.5)% of the ES-de- rived cells was stained positive green with ICG uptake. These cells were also stained magenta as a result of PAS reaction. In this paper, expression of coagulation factors VIII and IX mRNA in the ES-derived cells is documented. Therefore, ES cells might be developed as substitute donor cells for the therapy of coagulation factor deficiencies.

  10. Partial correction of a severe molecular defect in hemophilia A, because of errors during expression of the factor VIII gene

    Energy Technology Data Exchange (ETDEWEB)

    Young, M.; Antonarakis, S.E. [Univ. of Geneva (Switzerland); Inaba, Hiroshi [Tokyo Medical College (Japan)] [and others

    1997-03-01

    Although the molecular defect in patients in a Japanese family with mild to moderately severe hemophilia A was a deletion of a single nucleotide T within an A{sub 8}TA{sub 2} sequence of exon 14 of the factor VIII gene, the severity of the clinical phenotype did not correspond to that expected of a frameshift mutation. A small amount of functional factor VIII protein was detected in the patient`s plasma. Analysis of DNA and RNA molecules from normal and affected individuals and in vitro transcription/translation suggested a partial correction of the molecular defect, because of the following: (i) DNA replication/RNA transcription errors resulting in restoration of the reading frame and/or (ii) {open_quotes}ribosomal frameshifting{close_quotes} resulting in the production of normal factor VIII polypeptide and, thus, in a milder than expected hemophilia A. All of these mechanisms probably were promoted by the longer run of adenines, A{sub 10} instead of A{sub 8}TA{sub 2}, after the delT. Errors in the complex steps of gene expression therefore may partially correct a severe frameshift defect and ameliorate an expected severe phenotype. 36 refs., 6 figs.

  11. EFEKTIFITAS METODE MIND MAPPING UNTUK MENINGKATKAN PRESTASI BELAJAR FISIKA PADA SISWA KELAS VIII

    Directory of Open Access Journals (Sweden)

    Unggul Haryanto Nur Utomo

    2012-03-01

    Full Text Available This study aimed to determine the effectiveness of mind mappingmethod to improve the learning achievements of physics. The subjects ofthis study were students in grade VIII of SMP Muhammadiyah 8 Yogyakartawhich have average score of physics subjects below 6.5, and have theability to see and hear well. Data collection methods used in this study wasthe academic score on the physics subject of optical tools, documentation,observation and interview. This study used the experimental method ofpretest-posttest with control group design and used the t-test analysis.The results of paired sample t-test analysis in experimental group obtainedthat mind mapping method has highly significant positive effect in enhancingachievement of physics learning (t = -11.006, p = 0.000. The results ofthe paired sample t-test analysis in control group showed that conventionalmethod has insignificant influence on achievement enhancement of physicslearning (t = -1.941, p = 0.070 and the independent sample t-test in bothgroup found that there are significant difference of average (mean posttestscore between the experimental group and control group (t = 2.144, p =0.020. The results demonstrate mind mapping method is very effective inimproving the learning achievements of physics.

  12. Circumventing furin enhances factor VIII biological activity and ameliorates bleeding phenotypes in hemophilia models

    Science.gov (United States)

    Siner, Joshua I.; Samelson-Jones, Benjamin J.; Crudele, Julie M.; French, Robert A.; Lee, Benjamin J.; Zhou, Shanzhen; Merricks, Elizabeth; Raymer, Robin; Camire, Rodney M.; Arruda, Valder R.

    2016-01-01

    Processing by the proprotein convertase furin is believed to be critical for the biological activity of multiple proteins involved in hemostasis, including coagulation factor VIII (FVIII). This belief prompted the retention of the furin recognition motif (amino acids 1645–1648) in the design of B-domain–deleted FVIII (FVIII-BDD) products in current clinical use and in the drug development pipeline, as well as in experimental FVIII gene therapy strategies. Here, we report that processing by furin is in fact deleterious to FVIII-BDD secretion and procoagulant activity. Inhibition of furin increases the secretion and decreases the intracellular retention of FVIII-BDD protein in mammalian cells. Our new variant (FVIII-ΔF), in which this recognition motif is removed, efficiently circumvents furin. FVIII-ΔF demonstrates increased recombinant protein yields, enhanced clotting activity, and higher circulating FVIII levels after adeno-associated viral vector–based liver gene therapy in a murine model of severe hemophilia A (HA) compared with FVIII-BDD. Moreover, we observed an amelioration of the bleeding phenotype in severe HA dogs with sustained therapeutic FVIII levels after FVIII-ΔF gene therapy at a lower vector dose than previously employed in this model. The immunogenicity of FVIII-ΔF did not differ from that of FVIII-BDD as a protein or a gene therapeutic. Thus, contrary to previous suppositions, FVIII variants that can avoid furin processing are likely to have enhanced translational potential for HA therapy. PMID:27734034

  13. Eradication of neutralizing antibodies to factor VIII in canine hemophilia A after liver gene therapy.

    Science.gov (United States)

    Finn, Jonathan D; Ozelo, Margareth C; Sabatino, Denise E; Franck, Helen W G; Merricks, Elizabeth P; Crudele, Julie M; Zhou, Shangzhen; Kazazian, Haig H; Lillicrap, David; Nichols, Timothy C; Arruda, Valder R

    2010-12-23

    Inhibitory antibodies to factor VIII (FVIII) are a major complication in the treatment of hemophilia A, affecting approximately 20% to 30% of patients. Current treatment for inhibitors is based on long-term, daily injections of large amounts of FVIII protein. Liver-directed gene therapy has been used to induce antigen-specific tolerance, but there are no data in hemophilic animals with pre-existing inhibitors. To determine whether sustained endogenous expression of FVIII could eradicate inhibitors, we injected adeno-associated viral vectors encoding canine FVIII (cFVIII) in 2 strains of inhibitor hemophilia A dogs. In 3 dogs, a transient increase in inhibitor titers (up to 7 Bethesda Units [BU]) at 2 weeks was followed by continuous decline to complete disappearance within 4-5 weeks. Subsequently, an increase in cFVIII levels (1.5%-8%), a shortening of clotting times, and a reduction (> 90%) of bleeding episodes were observed. Immune tolerance was confirmed by lack of antibody formation after repeated challenges with cFVIII protein and normal protein half-life. A fourth dog exhibited a strong early anamnestic response (216 BU), with slow decline to 0.8 BU and cFVIII antigen detection by 18 months after vector delivery. These data suggest that liver gene therapy has the potential to eradicate inhibitors and could improve the outcomes of hemophilia A patients.

  14. Potential for cellular stress response to hepatic factor VIII expression from AAV vector

    Directory of Open Access Journals (Sweden)

    Irene Zolotukhin

    2016-01-01

    Full Text Available Hemophilia A and B are coagulation disorders resulting from the loss of functional coagulation factor VIII (FVIII or factor IX proteins, respectively. Gene therapy for hemophilia with adeno-associated virus vectors has shown efficacy in hemophilia B patients. Although hemophilia A patients are more prevalent, the development of therapeutic adeno-associated virus vectors has been impeded by the size of the F8 cDNA and impaired secretion of FVIII protein. Further, it has been reported that over-expression of the FVIII protein induces endoplasmic reticulum stress and activates the unfolded protein response pathway both in vitro and in hepatocytes in vivo, presumably due to retention of misfolded FVIII protein within the endoplasmic reticulum. Engineering of the F8 transgene, including removal of the B domain (BDD-FVIII and codon optimization, now allows for the generation of adeno-associated virus vectors capable of expressing therapeutic levels of FVIII. Here we sought to determine if the risks of inducing the unfolded protein response in murine hepatocytes extend to adeno-associated virus gene transfer. Although our data show a mild activation of unfolded protein response markers following F8 gene delivery at a certain vector dose in C57BL/6 mice, it was not augmented upon further elevated dosing, did not induce liver pathology or apoptosis, and did not impact FVIII immunogenicity.

  15. Improvement of fibrin clot structure after factor VIII injection in haemophilia A patients treated on demand.

    Science.gov (United States)

    Antovic, Aleksandra; Mikovic, Danijela; Elezovic, Ivo; Zabczyk, Michael; Hutenby, Kjell; Antovic, Jovan P

    2014-04-01

    Patients with haemophilia A have seriously impaired thrombin generation due to an inherited deficiency of factor (F)VIII, making them form unstable fibrin clots that are unable to maintain haemostasis. Data on fibrin structure in haemophilia patients remain limited. Fibrin permeability, assessed by a flow measurement technique, was investigated in plasma from 20 patients with severe haemophilia A treated on demand, before and 30 minutes after FVIII injection. The results were correlated with concentrations of fibrinogen, FVIII and thrombin-activatable fibrinolysis inhibitor (TAFI), and global haemostatic markers: endogenous thrombin potential (ETP) and overall haemostatic potential (OHP). Fibrin structure was visualised using scanning electron microscopy (SEM). The permeability coefficient Ks decreased significantly after FVIII treatment. Ks correlated significantly with FVIII levels and dosage, and with ETP, OHP and levels of TAFI. SEM images revealed irregular, porous fibrin clots composed of thick and short fibers before FVIII treatment. The clots had recovered after FVIII replacement almost to levels in control samples, revealing compact fibrin with smaller intrinsic pores. To the best of our knowledge, this is the first description of fibrin porosity and structure before and after FVIII treatment of selected haemophilia patients. It seems that thrombin generation is the main determinant of fibrin structure in haemophilic plasma.

  16. PENINGKATAN KUALITAS BERMAIN PERAN SISWA KELAS VIII SMP 1 TEPUS DENGAN TEKNIK LEARNING GAME

    Directory of Open Access Journals (Sweden)

    Sutrisno Sutrisno

    2014-05-01

    Full Text Available Penelitian ini bertujuan untuk mendeskripsikan peningkatan kualitas proses dan hasil pembelajaran bermain peran siswa kelas VIII SMP dengan menggunakan teknik Learning Game. Game yang digunakan adalah permainan Joko Tingkir dan permainan Tebak Peran. Penelitian ini merupakan penelitian tindakan kelas (PTK. Guru bertindak sebagai peneliti yang bekerja sama dengan kolaborator, yakni guru Bahasa Indonesia dan Kepala Sekolah SMP 1 Tepus. Subjek penelitian yang dikenai tindakan adalah siswa kelas VIIIA SMP 1 Tepus Gunungkidul, DIY, tahun ajaran 2011/2012 yang berjumlah 34 siswa. Penelitian ini terdiri atas dua siklus. Setiap siklus dibagi menjadi tiga kali pertemuan. Prosedur pelaksanaan setiap siklus terdiri atas empat tahap, yakni perencanaan, pelaksanaan tindakan, pengamatan, dan refleksi. Teknik pengumpulan data yang digunakan adalah angket, wawan-cara, pengamatan, dan pencermatan dokumen. Analisis dilakukan dengan teknik deskriptif kualitatif yang didukung oleh data kuantitatif.  Hasil penelitian menunjukkan bahwa dengan teknik Learning Game dalam pembelajaran bermain peran, kualitas proses dan hasil pembelajaran meningkat. Peningkatan kualitas proses pembelajaran meliputi aspek interaksi dan komunikasi dalam pembelajaran, kerja sama dalam kelompok, dan keseriusan dalam mengikuti pembelajaran. Adapun peningkatan kualitas hasil pembelajaran meliputi kemampuan vokal, intonasi, ekspresi, dan gestur. Keempat kemampuan tersebut masing-masing mengalami peningkatan nilai rata-rata lebih dari 25%. Hal ini sesuai dengan kriteria keberhasilan tindakan yang ditetapkan. Kata Kunci: bermain peran, teknik Learning Game

  17. Potential for cellular stress response to hepatic factor VIII expression from AAV vector

    Science.gov (United States)

    Zolotukhin, Irene; Markusic, David M; Palaschak, Brett; Hoffman, Brad E; Srikanthan, Meera A; Herzog, Roland W

    2016-01-01

    Hemophilia A and B are coagulation disorders resulting from the loss of functional coagulation factor VIII (FVIII) or factor IX proteins, respectively. Gene therapy for hemophilia with adeno-associated virus vectors has shown efficacy in hemophilia B patients. Although hemophilia A patients are more prevalent, the development of therapeutic adeno-associated virus vectors has been impeded by the size of the F8 cDNA and impaired secretion of FVIII protein. Further, it has been reported that over-expression of the FVIII protein induces endoplasmic reticulum stress and activates the unfolded protein response pathway both in vitro and in hepatocytes in vivo, presumably due to retention of misfolded FVIII protein within the endoplasmic reticulum. Engineering of the F8 transgene, including removal of the B domain (BDD-FVIII) and codon optimization, now allows for the generation of adeno-associated virus vectors capable of expressing therapeutic levels of FVIII. Here we sought to determine if the risks of inducing the unfolded protein response in murine hepatocytes extend to adeno-associated virus gene transfer. Although our data show a mild activation of unfolded protein response markers following F8 gene delivery at a certain vector dose in C57BL/6 mice, it was not augmented upon further elevated dosing, did not induce liver pathology or apoptosis, and did not impact FVIII immunogenicity. PMID:27738644

  18. Immunogenicity and pharmacokinetic studies of recombinant factor VIII containing lipid cochleates.

    Science.gov (United States)

    Kosloski, Matthew P; Peng, Aaron; Varma, Prashant R; Fathallah, Anas M; Miclea, Razvan D; Mager, Donald E; Balu-iyer, Sathy V

    2011-05-01

    Replacement therapy using recombinant factor VIII (rFVIII) is currently the most common therapy for hemophilia A, a bleeding disorder caused by the deficiency of FVIII. However, 15-30% of patients develop inhibitory antibodies against administered rFVIII, which complicates the therapy. Encapsulation or association of protein with lipidic structures can reduce this immune response. Previous studies developed and characterized rFVIII-containing phosphatidylserine (PS) cochleate cylinders using biophysical techniques. It was hypothesized that these structures may provide a reduction in immunogenicity while avoiding the rapid clearance by the reticuloendothelial system (RES) previously observed with liposomal vesicles of similar composition. This study investigated in vivo behavior of the cochleates containing rFVIII including immunogenicity and pharmacokinetics in hemophilia A mice. The rFVIII-cochleate complex significantly reduced the level of inhibitory antibody developed against rFVIII following intravenous (i.v.) administration. Pharmacokinetic modeling allowed assessment of in vivo release kinetics. Cochleates acted as a delayed release delivery vehicle with an input peak of cochleates showed limited RES uptake and associated rFVIII displayed a similar disposition to the free protein upon release from the structure. Incomplete disassociation from the complex limits systemic availability of the protein. Further formulation efforts are warranted to regulate the rate and extent of release of rFVIII from cochleate complexes.

  19. Continuous infusion of porcine factor VIII: stability, microbiological safety and clinical experience.

    Science.gov (United States)

    DiMichele, D M; Gorman, P O; Kasper, C K; Mannucci, P M; Santagostino, E; Hay, C R M

    2002-01-01

    Porcine factor VIII (pFVIII) is an effective haemostatic treatment for bleeding in selected patients with FVIII inhibitors. Its use is sometimes associated with a transient fall in platelet count and transfusion reactions, the risk of which may be related to the rate of administration. Theoretical considerations suggest that the administration of pFVIII by continuous infusion should be effective, and could have pharmacokinetic advantages that lead to an improvement in the side-effect profile. The results of a retrospective survey of continuous infusion of pFVIII with respect to clinical safety and efficacy are reported. Porcine FVIII stability and microbiological studies are included. It is concluded that pFVIII given by continuous infusion is safe and effective. The risk of transfusion reactions and fall in platelet count appears to be reduced, compared with bolus administration. Stability studies showed that pFVIII activity declined at room temperature, most rapidly in the dilute solution (5-10 U mL(-1)). More concentrated mixtures showed acceptable stability for up to 24 h using a variety of infusion devices. Various concentrations of pFVIII did not support the growth of Escherichia coli or Staphylococcus aureus. These observations suggest that the porcine factor is suitable for continuous infusion (CI).

  20. Evolution of recombinant factor VIII safety: KOGENATE and Kogenate FS/Bayer.

    Science.gov (United States)

    Lusher, Jeanne M; Scharrer, Inge

    2009-11-01

    The use of factor VIII (FVIII) concentrates in the treatment of hemophilia A has raised important safety issues, historically of pathogen transmission and increasingly of inhibitor development to FVIII treatment. While manufacturing processes of current recombinant FVIII products have been shaped entirely around preventing pathogen transmission, the same modifications that afford a greater margin of safety could affect immunogenicity of the product, consequences of which could only be seen through long-term clinical experience. This review summarizes pathogen safety and inhibitor reports from clinical trials, post-marketing surveillance studies, and study reports on KOGENATE and its successor, Kogenate FS/Bayer. Although KOGENATE and Kogenate FS/Bayer are nearly identical products, subtle manufacturing improvements to address the need for greater margins of safety from a pathogen transmission perspective have also led to a potentially improved immunogenicity profile (15% in previously untreated/minimally treated patients with severe hemophilia A for Kogenate FS/Bayer). Notably, there has been no occurrence of pathogen contamination, and minimal de novo inhibitor formation in previously treated patients throughout the use of both products. Overall, KOGENATE and Kogenate FS/Bayer have a long history of safety in a variety of clinical settings, including treatment of bleeding, surgical management, and prophylaxis therapy.

  1. Phonon dynamics in type-VIII silicon clathrates: Beyond the rattler concept

    Science.gov (United States)

    Norouzzadeh, Payam; Myles, Charles W.; Vashaee, Daryoosh

    2017-05-01

    Clathrates can form a type of guest-host solid structures that, unlike most crystalline solids, have very low thermal conductivity. It is generally thought that the guest atoms caged inside the host framework act as "rattlers" and induce lattice dynamics disorders responsible for the small thermal conductivity. We performed a systematic study of the lattice dynamical properties of type-VIII clathrates with alkali and alkaline-earth guests, i.e., X8S i46 (X =Na , K, Rb, Cs, Ca, Sr, and Ba). The energy dependent participation ratio (PR) and the atomic participation ratio of phonon modes extracted from density functional theory calculations revealed that the rattler concept is not adequate to describe the effect of fillers as they manifest strong hybridization with the framework. For the case of heavy fillers, such as Rb, Sr, Cs, and Ba, a phonon band gap was formed between the acoustic and optical branches. The calculated PR indicated that the fillers suppress the acoustic phonon modes and change the energy transport mechanism from propagative to diffusive or localized resulting in "phonon-glass" characteristics. This effect is stronger for the heavy fillers. Furthermore, in all cases, the guest insertion depressed the phonon bandwidth, reduced the Debye temperature, and reduced the phonon group velocity, all of which should lead to reduction of the thermal conductivity.

  2. BAY 81-8973, a full-length recombinant factor VIII: manufacturing processes and product characteristics.

    Science.gov (United States)

    Garger, S; Severs, J; Regan, L; Hesslein, A; Ignowski, J; Wu, P; Long, E; Gupta, S; Liu, S; Wang, W

    2017-03-01

    BAY 81-8973 (Kovaltry(®) , Bayer, Berkeley, CA, USA) is an unmodified, full-length recombinant human factor VIII (FVIII) approved for prophylaxis and on-demand treatment of bleeding episodes in patients with haemophilia A. The BAY 81-8973 manufacturing process is based on the process used for sucrose-formulated recombinant FVIII (rFVIII-FS), with changes and enhancements made to improve production efficiency, further augment pathogen safety, and eliminate animal- and human-derived raw materials from the production processes. The baby hamster kidney cell line used for BAY 81-8973 was developed by introducing the gene for human heat shock protein 70 into the rFVIII-FS cell line, a change that improved cell line robustness and productivity. Pathogen safety was enhanced by including a 20-nm filtration step, which can remove viruses, transmissible spongiform encephalopathy agents and potential protein aggregates. No human- or animal-derived proteins are added to the cell culture process, purification or final formulation. The BAY 81-8973 manufacturing process results in a product of enhanced purity with a consistently high degree of sialylation of N-linked glycans on the molecular surface. The innovative manufacturing techniques used for BAY 81-8973 yield an effective rFVIII product with a favourable safety profile for treatment of haemophilia A. © 2016 Bayer. Haemophilia Published by John Wiley & Sons Ltd.

  3. A population pharmacokinetic model for perioperative dosing of factor VIII in hemophilia A patients.

    Science.gov (United States)

    Hazendonk, Hendrika; Fijnvandraat, Karin; Lock, Janske; Driessens, Mariëtte; van der Meer, Felix; Meijer, Karina; Kruip, Marieke; Gorkom, Britta Laros-van; Peters, Marjolein; de Wildt, Saskia; Leebeek, Frank; Cnossen, Marjon; Mathôt, Ron

    2016-10-01

    The role of pharmacokinetic-guided dosing of factor concentrates in hemophilia is currently a subject of debate and focuses on long-term prophylactic treatment. Few data are available on its impact in the perioperative period. In this study, a population pharmacokinetic model for currently registered factor VIII concentrates was developed for severe and moderate adult and pediatric hemophilia A patients (FVIII levels modeling was performed using non-linear mixed-effects modeling. Population pharmacokinetic parameters were estimated in 75 adults undergoing 140 surgeries (median age: 48 years; median weight: 80 kg) and 44 children undergoing 58 surgeries (median age: 4.3 years; median weight: 18.5 kg). Pharmacokinetic profiles were best described by a two-compartment model. Typical values for clearance, intercompartment clearance, central and peripheral volume were 0.15 L/h/68 kg, 0.16 L/h/68 kg, 2.81 L/68 kg and 1.90 L/68 kg. Interpatient variability in clearance and central volume was 37% and 27%. Clearance decreased with increasing age (Pmodel describes the perioperative pharmacokinetics of various FVIII concentrates, allowing individualization of perioperative FVIII therapy for severe and moderate hemophilia A patients by Bayesian adaptive dosing. Copyright© Ferrata Storti Foundation.

  4. Library holdings for EX0909 Legs 1 through 4: Mapping Field Trials V through VIII Hawaiian Islands on NOAA Ship Okeanos Explorer between August 21, 2009 and November 15, 2009

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Library Catalog may include: Data Management Plans, Cruise Plans, Cruise Summary Reports, Scientific "Quick Look Reports", Video Annotation Logs, Image Collections,...

  5. Cruising Venues as a Context for HIV Risky Behavior Among Men Who Have Sex With Men.

    Science.gov (United States)

    Gama, Ana; Abecasis, Ana; Pingarilho, Marta; Mendão, Luís; Martins, Maria O; Barros, Henrique; Dias, Sónia

    2016-03-17

    We examined differences in sexual risk behaviors, HIV prevalence, and demographic characteristics between men who have sex with men (MSM) who visit different types of venues to meet sexual partners, and identified correlates of high-risk behaviors. A cross-sectional behavioral survey was conducted with a venue-based sample of 1011 MSM in Portugal. Overall, 36.3 % of MSM usually visit cruising venues to meet sexual partners (63.7 % only visit social gay venues). Cruising venues' visitors reported higher HIV prevalence (14.6 % [95 % CI 11-18 %] vs. 5.5 % [95 % CI 4-7 %]). Visiting cruising venues was more likely among those older, reporting high number of male sexual partners, group sex, and unprotected anal sex with a partner whose HIV status was unknown. Cruising venues play an important role in increasing risk of HIV transmission among MSM who frequent them. Venue-focused behavioral interventions that promote healthy sexual behaviors are needed.

  6. Explicit MPC design and performance-based tuning of an Adaptive Cruise Control Stop-&-Go

    NARCIS (Netherlands)

    Naus, G.J.L.; Ploeg, J.; Molengraft, M.J.G. van de; Steinbuch, M.

    2008-01-01

    This paper presents the synthesis, the implementation and the performance-based tuning of an Adaptive Cruise Control (ACC) Stop-&-Go (S&G) design. A Model Predictive Control (MPC) framework is adopted to design the controller. Performance of the controller is evaluated, distinguishing between comfor

  7. Data report of the first cruise of the Marion Off-shore Ecological Study (MOES-1)

    CSIR Research Space (South Africa)

    Duncombe-Rae, CM

    1989-01-01

    Full Text Available The first cruise of the Marion Off-shore Ecological Study (MOES-I), during April and May 1987, was a multi-disciplinary effort aimed at gaining a further understanding of the relationships between productivity and the environment in the vicinity...

  8. E-Services and Positioning of Passenger Ports in the Context of Cruise Tourism Promotion

    Directory of Open Access Journals (Sweden)

    Andriela Vitić-Ćetković

    2014-02-01

    Full Text Available The paper positions the passenger sea ports in the context of cruise tourism on the basis of e-services they offer. The e-services of eleven passenger ports are categorized and then quantitatively evaluated by binary and ranking approaches. In general, the port e-services might be categorized according to their functionality as navigational, ship and passenger-related ones, logistics, business, marketing, entertainment, security, safety, environmental, etc. These services can be bidirectional informational and/or transactional. In this paper, only those port e-services related directly to the passengers’ needs, within the frame of cruise tourism, are taken into consideration and categorized as core, or as value-added ones, and as informational and/or transactional ones. Then, each of them is assigned an appropriate binary value (0/1, depending on whether the considered passenger port offers the related e-service or not. These values are employed in the evaluation of the analyzed passenger port e-services offered, and as a base for their positioning. The appropriate weights coefficients, obtained by ranking (Saaty method, were used in the process of the considered port final positioning on the cruise tourism e-market. Some additional analyses and recommendations in the direction of further positioning and promotion of the port of Kotor (Montenegro, as rising cruise tourism port (destination, are given as well.

  9. The role of finite-difference methods in design and analysis for supersonic cruise

    Science.gov (United States)

    Townsend, J. C.

    1976-01-01

    Finite-difference methods for analysis of steady, inviscid supersonic flows are described, and their present state of development is assessed with particular attention to their applicability to vehicles designed for efficient cruise flight. Current work is described which will allow greater geometric latitude, improve treatment of embedded shock waves, and relax the requirement that the axial velocity must be supersonic.

  10. Experiencing Work: Supporting the Undergraduate Hospitality, Tourism and Cruise Management Student on an Overseas Work Placement

    Science.gov (United States)

    Gibson, Philip; Busby, Graham

    2009-01-01

    This paper reports on a funded research project into the experiences of tourism, hospitality and cruise management students on internship outside the UK as part of their British university degree between 2007 and 2009. The research reflected on the perceptions of students, course managers, placement officers and members of university placement…

  11. Cosmic Rays with Portable Geiger Counters: From Sea Level to Airplane Cruise Altitudes

    Science.gov (United States)

    Blanco, Francesco; La Rocca, Paola; Riggi, Francesco

    2009-01-01

    Cosmic ray count rates with a set of portable Geiger counters were measured at different altitudes on the way to a mountain top and aboard an aircraft, between sea level and cruise altitude. Basic measurements may constitute an educational activity even with high school teams. For the understanding of the results obtained, simulations of extensive…

  12. Antarctic cruise tourism: the paradoxes of ambassadorship, "last chance tourism" and greenhouse gas emissions

    NARCIS (Netherlands)

    Eijgelaar, E.; Thaper, C.; Peeters, P.M.

    2010-01-01

    This paper examines a paradoxical issue in tourism's adaptation to climate change and emissions reduction demands. Operators increasingly take tourists to destinations threatened by climate change, with Antarctica and other polar regions as favourites and cruise ship and aircraft as main transport m

  13. Traffic flow impacts of adaptive cruise control deactivation and (Re)activation with cooperative driver behavior

    NARCIS (Netherlands)

    Klunder, G.; Li, M.; Minderhoud, M.

    2009-01-01

    In 2006 in the Netherlands, a field operational test was carried out to study the effect of adaptive cruise control (ACC) and lane departure warning on driver behavior and traffic flow in real traffic. To estimate the effect for larger penetration rates, simulations were needed. For a reliable

  14. Traffic flow impacts of adaptive cruise control deactivation and (Re)activation with cooperative driver behavior

    NARCIS (Netherlands)

    Klunder, G.; Li, M.; Minderhoud, M.

    2009-01-01

    In 2006 in the Netherlands, a field operational test was carried out to study the effect of adaptive cruise control (ACC) and lane departure warning on driver behavior and traffic flow in real traffic. To estimate the effect for larger penetration rates, simulations were needed. For a reliable impac

  15. Antarctic cruise tourism: the paradoxes of ambassadorship, "last chance tourism" and greenhouse gas emissions

    NARCIS (Netherlands)

    Eijgelaar, E.; Thaper, C.; Peeters, P.M.

    2010-01-01

    This paper examines a paradoxical issue in tourism's adaptation to climate change and emissions reduction demands. Operators increasingly take tourists to destinations threatened by climate change, with Antarctica and other polar regions as favourites and cruise ship and aircraft as main transport

  16. 1st Science-Industry platform on expedition cruise tourism in Svalbard

    NARCIS (Netherlands)

    Lamers, M.A.J.; Olsen, J.; Hovelsrud, G.; Lang, I.; Jorgensen, F.

    2014-01-01

    The interest in Svalbard as a cruise tourism destination has increased gradually over the past decades, leading to a range of opportunities and challenges for marine and terrestrial ecosystems, communities, and regulatory systems on Svalbard and elsewhere in the Arctic (e.g. Greenland, Iceland,

  17. FRONTS CRUISE: Leg I: 11 July 1985, Leg II: 12-23 July 1985.

    Science.gov (United States)

    1986-10-01

    de Pesca (INP), the Secretaria de Marina, and the Centro de Investi- gacion Cientifica y de Educacion Superior de Ensenada (CICESE). The purpose of...the Secretaria de Marina, and the Centro de Investi- gacion Cientifica y de Educacion Superior de Ensenada (CICESE). The purpose of the cruise was

  18. 33 CFR 165.1154 - Security Zones; Cruise Ships, San Pedro Bay, California.

    Science.gov (United States)

    2010-07-01

    ... anchored at a designated anchorage either inside the Federal breakwaters bounding San Pedro Bay or outside... instructions of the Captain of the Port or his or her designated representative. (3) When a cruise ship approaches within 100 yards of a vessel that is moored, or anchored, the stationary vessel must stay...

  19. A collision model for safety evaluation of autonomous intelligent cruise control.

    Science.gov (United States)

    Touran, A; Brackstone, M A; McDonald, M

    1999-09-01

    This paper describes a general framework for safety evaluation of autonomous intelligent cruise control in rear-end collisions. Using data and specifications from prototype devices, two collision models are developed. One model considers a train of four cars, one of which is equipped with autonomous intelligent cruise control. This model considers the car in front and two cars following the equipped car. In the second model, none of the cars is equipped with the device. Each model can predict the possibility of rear-end collision between cars under various conditions by calculating the remaining distance between cars after the front car brakes. Comparing the two collision models allows one to evaluate the effectiveness of autonomous intelligent cruise control in preventing collisions. The models are then subjected to Monte Carlo simulation to calculate the probability of collision. Based on crash probabilities, an expected value is calculated for the number of cars involved in any collision. It is found that given the model assumptions, while equipping a car with autonomous intelligent cruise control can significantly reduce the probability of the collision with the car ahead, it may adversely affect the situation for the following cars.

  20. Cooperative adaptive cruise control: Network-aware analysis of string stability

    NARCIS (Netherlands)

    Oncu, S.; Ploeg, J.; Wouw, N. van de; Nijmeijer, H.

    2014-01-01

    In this paper, we consider a Cooperative Adaptive Cruise Control (CACC) system, which regulates intervehicle distances in a vehicle string, for achieving improved traffic flow stability and throughput. Improved performance can be achieved by utilizing information exchange between vehicles through wi

  1. Experiencing Work: Supporting the Undergraduate Hospitality, Tourism and Cruise Management Student on an Overseas Work Placement

    Science.gov (United States)

    Gibson, Philip; Busby, Graham

    2009-01-01

    This paper reports on a funded research project into the experiences of tourism, hospitality and cruise management students on internship outside the UK as part of their British university degree between 2007 and 2009. The research reflected on the perceptions of students, course managers, placement officers and members of university placement…

  2. The Primi Project: August-September 2009 Validation Cruise On Oil Spill Detection And Fate

    Science.gov (United States)

    Santoleri, R.; Bignami, F.; Bohm, E.; Nichio, F.; De Dominicis, M.; Ruggieri, G.; Marulllo, S.; Trivero, P.; Zambianchi, E.; Archetti, R.; Adamo, M.; Biamino, W.; Borasi, M.; Buongiorno Nardelli, B.; Cavagnero, M.; Colao, F.; Colella, S.; Coppini, G.; Debettio, V.; De Carolis, G.; Forneris, V.; Griffa, A.; Iacono, R.; Lombardi, E.; Manzella, G.; Mercantini, A.; Napolitano, E.; Pinardi, N.; Pandiscia, G.; Pisano, A.; Rupolo, V.; Reseghetti, F.; Sabia, L.; Sorgente, R.; Sprovieri, M.; Terranova, G.; Trani, M.; Volpe, G.

    2010-04-01

    In the framework of the ASI PRIMI Project, CNR- ISAC, in collaboration with the PRIMI partners, organized a validation cruise for the PRIMI oil spill monitoring and forecasting system on board the CNR R/V Urania. The cruise (Aug. 6 - Sept. 7 2009) took place in the Sicily Strait, an area affected by large oil tanker traffic. The cruise plan was organized in order to have the ship within the selected SAR image frames at acquisition time so that the ship could move toward the oil slick and verify it via visual and instrumental inspection. During the cruise, several oil spills, presumably being the result of illegal tank washing, were detected by the PRIMI system and were verified in situ. Preliminary results indicate that SAR and optical satellites are able to detect heavy and thin film oil spills, the maturity of oil spill forecasting models and that further work combining satellite, model and in situ data is necessary to assess the spill severity from the signature in satellite imagery.

  3. Towards on-the-road implementation of cooperative adaptive cruise control

    NARCIS (Netherlands)

    Naus, G.J.L.; Vugts, R.; Ploeg, J.; Molengraft, R. van de; Steinbuch, M.

    2009-01-01

    This paper presents a setup for cooperative adaptive cruise control for which feasibility of the actual implementation is one of the main objectives. The approach considers communication with the directly preceding vehicle only, can deal with heterogeneous traffic, accounts for communication delay,

  4. 1st Science-Industry platform on expedition cruise tourism in Svalbard

    NARCIS (Netherlands)

    Lamers, M.A.J.; Olsen, J.; Hovelsrud, G.; Lang, I.; Jorgensen, F.

    2014-01-01

    The interest in Svalbard as a cruise tourism destination has increased gradually over the past decades, leading to a range of opportunities and challenges for marine and terrestrial ecosystems, communities, and regulatory systems on Svalbard and elsewhere in the Arctic (e.g. Greenland, Iceland, Russ

  5. 1st Science-Industry platform on expedition cruise tourism in Svalbard

    NARCIS (Netherlands)

    Lamers, M.A.J.; Olsen, J.; Hovelsrud, G.; Lang, I.; Jorgensen, F.

    2014-01-01

    The interest in Svalbard as a cruise tourism destination has increased gradually over the past decades, leading to a range of opportunities and challenges for marine and terrestrial ecosystems, communities, and regulatory systems on Svalbard and elsewhere in the Arctic (e.g. Greenland, Iceland, Russ

  6. Short-circuiting cruise tourism practices along the Russian Barents Sea coast? The case of Arkhangelsk

    NARCIS (Netherlands)

    Lamers, M.A.J.; Pashkevich, A.

    2015-01-01

    The growth and popularity of polar cruise tourism in the Arctic region have raised expectations about the opportunities in this part of the world. However, the existing academic literature has never ventured further than to recall these expectations and opportunities, which means that there is hardl

  7. Heat Flow Data Cruise MD72 RV Marion Dufresne over the Mascarene Ridge

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data were gathered by the R/V Marion Dufresne in May and June of 1992 over the Mascarene Ridge in the Indian Ocean on cruise MD72/MASCAFLUX. Heat flow measurements...

  8. Design and implementation of parameterized adaptive cruise control: An explicit model predictive control approach

    NARCIS (Netherlands)

    Naus, G.J.L.; Ploeg, J.; Molengraft, M.J.G. van de; Heemels, W.P.M.H.; Steinbuch, M.

    2010-01-01

    The combination of different characteristics and situation-dependent behavior cause the design of adaptive cruise control (ACC) systems to be time consuming. This paper presents a systematic approach for the design of a parameterized ACC, based on explicit model predictive control. A unique feature

  9. Extensive Nosocomial Transmission of Measles Originating in Cruise Ship Passenger, Sardinia, Italy, 2014.

    Science.gov (United States)

    Filia, Antonietta; Bella, Antonino; Cadeddu, Giovanna; Milia, Maria Rafaela; Del Manso, Martina; Rota, Maria Cristina; Magurano, Fabio; Nicoletti, Loredana; Declich, Silvia

    2015-08-01

    We report a measles outbreak in Sardinia, Italy, that originated in a cruise ship passenger. The outbreak showed extensive nosocomial transmission (44 of 80 cases). To minimize nosocomial transmission, health care facilities should ensure that susceptible health care workers are vaccinated against measles and should implement effective infection control procedures.

  10. Carbon emissions from international cruise ship passengers' travel to and from New Zealand

    Energy Technology Data Exchange (ETDEWEB)

    Howitt, Oliver J.A.; Revol, Vincent G.N.; Smith, Inga J.; Rodger, Craig J. [Department of Physics, University of Otago, PO Box 56, Dunedin (New Zealand)

    2010-05-15

    Greenhouse gas emissions from international transport contribute to anthropogenic global warming, yet these emissions are not liable under the Kyoto Protocol. International attention is being given to quantifying such emissions. This paper presents the results of research into international cruise ship journeys to and from New Zealand. CO{sub 2} emissions from such journeys were calculated using an activity based, or 'bottom-up', model. Emissions factors for individual journeys by cruise ships to or from New Zealand in 2007 ranged between 250 and 2200 g of CO{sub 2} per passenger-kilometre (g CO{sub 2} per p-km), with a weighted mean of 390 g CO{sub 2} per p-km. The weighted mean energy use per passenger night for the 'hotel' function of these cruise vessels was estimated as 1600 MJ per visitor night, 12 times larger than the value for a land-based hotel. Using a simple price elasticities calculation, international cruise journeys for transport purposes were found to have a greater relative decrease in demand than plane journeys when the impact of carbon pricing was analysed. The potential to decrease the CO{sub 2} emissions per p-km was examined, and if passenger accommodation was compacted and some luxury amenities dispensed with values similar to those of economy-class air travel were obtained. (author)

  11. RRS Discovery Cruise 249, 19 Aug-10 Sep 2000. History of sedimentation in the Gulf of Cadiz: investigations with the SOC giant piston corer

    OpenAIRE

    Weaver, P.P.E.

    2003-01-01

    This was the third cruise using the new SOC giant piston corer. The objectives were to complete the testing of the giant piston corer which was begun on cruise D219 in November/December 1995 and cruise D225 in February/March 1997, and to collect giant piston cores from the Gulf of Cadiz on sites selected from the TOBI survey carried out on cruise D244 in December 1999. As on previous cruises we had problems with both the ships equipment (outboard sheave on coring gantry), and the giant pist...

  12. Comparison of Hygroscopicity, Volatility, and Mixing State of Submicrometer Particles between Cruises over the Arctic Ocean and the Pacific Ocean.

    Science.gov (United States)

    Kim, Gibaek; Cho, Hee-Joo; Seo, Arom; Kim, Dohyung; Gim, Yeontae; Lee, Bang Yong; Yoon, Young Jun; Park, Kihong

    2015-10-20

    Ship-borne measurements of ambient aerosols were conducted during an 11 937 km cruise over the Arctic Ocean (cruise 1) and the Pacific Ocean (cruise 2). A frequent nucleation event was observed during cruise 1 under marine influence, and the abundant organic matter resulting from the strong biological activity in the ocean could contribute to the formation of new particles and their growth to a detectable size. Concentrations of particle mass and black carbon increased with increasing continental influence from polluted areas. During cruise 1, multiple peaks of hygroscopic growth factor (HGF) of 1.1-1.2, 1.4, and 1.6 were found, and higher amounts of volatile organic species existed in the particles compared to that during cruise 2, which is consistent with the greater availability of volatile organic species caused by the strong oceanic biological activity (cruise 1). Internal mixtures of volatile and nonhygroscopic organic species, nonvolatile and less-hygroscopic organic species, and nonvolatile and hygroscopic nss-sulfate with varying fractions can be assumed to constitute the submicrometer particles. On the basis of elemental composition and morphology, the submicrometer particles were classified into C-rich mixture, S-rich mixture, C/S-rich mixture, Na-rich mixture, C/P-rich mixture, and mineral-rich mixture. Consistently, the fraction of biological particles (i.e., P-containing particles) increased when the ship traveled along a strongly biologically active area.

  13. Determination of Close Loop System Stability in Automobile Adaptive Cruise Control Systems

    Directory of Open Access Journals (Sweden)

    Owunna Ikechukwu

    2016-07-01

    Full Text Available The beginning of the 21st century sees auto makers pursuing research in advanced features like collision warning and avoidance system into their product. Automotive cruise control system has been undergoing development in EU since the PROMETHEUS programme in the late 1980’s, and has currently metamorphous into Adaptive Cruise Control (ACC technology which is presently emerging in the automotive market as a convenience function intended to reduce driver workload. Adaptive cruise control is the first of the new generation of advanced driver’s assistance devices to reach the market, which partially automates the driver’s task and bringing the drivers comfort into perspective. It allows the host vehicle to maintain a set speed and distance from preceding vehicles by a forward object detection sensor. The forward object detection sensor is the focal point of the ACC system, which determines and regulates vehicle acceleration and deceleration through a powertrain torque control system and an automatic brake control system. This study presents overview of adaptive cruise control system, operation principles and the advantages of integrating ACC system in automobiles. Also, the system must be stable for optimum performance, and stability of a close loop system which the cruise system is an example, was determined by calculating the controller gain (K1, K2, K3 and substituting into the characteristic equations. The stability of a close loop system for the values of K1, K2 and K3 when substituted into the characteristic equation produced a negative real part. To achieve stability in close loop systems, all the poles must have negative real values and this is in line with the values obtain for p1, p2 and p3. From the pole zero plots of 1 = (-7 ± 7.14, 2 = (-7± 11.60 and 3 = (-0.08 and -13.91, stability of the system was achieved

  14. Severe Hemophilia A in a Male Old English Sheep Dog with a C→T Transition that Created a Premature Stop Codon in Factor VIII

    Science.gov (United States)

    Lozier, Jay N; Kloos, Mark T; Merricks, Elizabeth P; Lemoine, Nathaly; Whitford, Margaret H; Raymer, Robin A; Bellinger, Dwight A; Nichols, Timothy C

    2016-01-01

    Animals with hemophilia are models for gene therapy, factor replacement, and inhibitor development in humans. We have actively sought dogs with severe hemophilia A that have novel factor VIII mutations unlike the previously described factor VIII intron 22 inversion. A male Old English Sheepdog with recurrent soft-tissue hemorrhage and hemarthrosis was diagnosed with severe hemophilia A (factor VIII activity less than 1% of normal). We purified genomic DNA from this dog and ruled out the common intron 22 inversion; we then sequenced all 26 exons. Comparing the results with the normal canine factor VIII sequence revealed a C→T transition in exon 12 of the factor VIII gene that created a premature stop codon at amino acid 577 in the A2 domain of the protein. In addition, 2 previously described polymorphisms that do not cause hemophilia were present at amino acids 909 and 1184. The hemophilia mutation creates a new TaqI site that facilitates rapid genotyping of affected offspring by PCR and restriction endonuclease analyses. This mutation is analogous to the previously described human factor VIII mutation at Arg583, which likewise is a CpG dinucleotide transition causing a premature stop codon in exon 12. Thus far, despite extensive treatment with factor VIII, this dog has not developed neutralizing antibodies (‘inhibitors’) to the protein. This novel mutation in a dog gives rise to severe hemophilia A analogous to a mutation seen in humans. This model will be useful for studies of the treatment of hemophilia. PMID:27780008

  15. Self-reported stomach upset in travellers on cruise-based and land-based package holidays.

    Directory of Open Access Journals (Sweden)

    Naomi J Launders

    Full Text Available BACKGROUND: International travellers are at a risk of infectious diseases not seen in their home country. Stomach upsets are common in travellers, including on cruise ships. This study compares the incidence of stomach upsets on land- and cruise-based holidays. METHODS: A major British tour operator has administered a Customer Satisfaction Questionnaire (CSQ to UK resident travellers aged 16 or more on return flights from their holiday abroad over many years. Data extracted from the CSQ was used to measure self-reported stomach upset in returning travellers. RESULTS: From summer 2000 through winter 2008, 6,863,092 questionnaires were completed; 6.6% were from cruise passengers. A higher percentage of land-based holiday-makers (7.2% reported stomach upset in comparison to 4.8% of cruise passengers (RR = 1.5, p<0.0005. Reported stomach upset on cruises declined over the study period (7.1% in 2000 to 3.1% in 2008, p<0.0005. Over 25% of travellers on land-based holidays to Egypt and the Dominican Republic reported stomach upset. In comparison, the highest proportion of stomach upset in cruise ship travellers were reported following cruises departing from Egypt (14.8% and Turkey (8.8%. CONCLUSIONS: In this large study of self-reported illness both demographic and holiday choice factors were shown to play a part in determining the likelihood of developing stomach upset while abroad. There is a lower cumulative incidence and declining rates of stomach upset in cruise passengers which suggest that the cruise industry has adopted operations (e.g. hygiene standards that have reduced illness over recent years.

  16. ADAMTS13 content in plasma-derived factor VIII/von Willebrand factor concentrates.

    Science.gov (United States)

    Peyvandi, Flora; Mannucci, Pier M; Valsecchi, Carla; Pontiggia, Silvia; Farina, Claudio; Retzios, Anastassios D

    2013-10-01

    Thrombotic thrombocytopenic purpura (TTP) is a microangiopathy syndrome caused by a congenital or acquired deficiency of ADAMTS13, a plasma metalloprotease that cleaves von Willebrand factor (VWF) and thus prevents the formation of platelet-rich thrombi in the microcirculation. TTP can be fatal if not appropriately and timely treated with the infusion of fresh frozen plasma (FFP) or exchange plasmapheresis, that reverse the process of microangiopathy by removing anti-ADAMTS13 autoantibodies and replacing functional ADAMTS13. The treatment of TTP with FFP is not free from risks and must be administered in hospitals or clinics, owing to the substantial amount of plasma volume infused or exchanged and the frequent need of catheter application. Moreover, most FFPs are not subjected to treatments to remove or inactivate blood-borne infectious agents. A number of recent reports indicate that certain plasma-derived VWF-factor VIII (FVIII) concentrates are clinically effective in the treatment of congenital TTP. In this study, we measured ADAMTS13 levels in various plasma-derived VWF-FVIII concentrates, showing that Koate(®) -DVI (Grifols), contained relatively high amounts of ADAMTS13 and that Alphanate(®) (Grifols) was the closest other product in terms of protease content. Koate(®) -DVI contains, on average (five lots tested), 0.091 ± 0.007 Units of ADAMTS13 activity per IU of FVIII. On the basis of this analysis and other reports of VWF-FVIII concentrate utilization in congenital TTP, potential dosing, and future clinical developments are discussed.

  17. Factor VIII gene variants and inhibitor risk in African American hemophilia A patients.

    Science.gov (United States)

    Gunasekera, Devi; Ettinger, Ruth A; Nakaya Fletcher, Shelley; James, Eddie A; Liu, Maochang; Barrett, John C; Withycombe, Janice; Matthews, Dana C; Epstein, Melinda S; Hughes, Richard J; Pratt, Kathleen P

    2015-08-13

    African American hemophilia A (HA) patients experience a higher incidence of neutralizing anti-factor VIII (FVIII) antibodies ("inhibitors") vis-à-vis white patients. Nonsynonymous single-nucleotide polymorphisms (ns-SNPs) in the F8 gene encoding FVIII-H484, FVIII-E1241, and FVIII-V2238 are more prevalent in African Americans. This study tested the hypothesis that immune responses to these sites provoke inhibitors. Blood samples were obtained from 174 African American and 198 white HA subjects and their F8 gene sequences determined. Major histocompatibility complex class II binding and T-cell recognition of polymorphic sequences were evaluated using quantitative binding assays and HLA-DRB1 tetramers. Peptides corresponding to 4 common ns-SNPs showed limited binding to 11 HLA-DRB1 proteins. CD4 T cells from 22 subjects treated with FVIII products having sequences at residues FVIII-484, 1241, and 2238 differing from those of putative proteins encoded by their F8 genes did not show high-avidity tetramer binding, whereas positive-control staining of tetanus-specific CD4 T cells was routinely successful. African Americans with an intron-22 inversion mutation showed a 2-3 times-higher inhibitor incidence than whites with the same mutation (odds ratio = 2.3 [1.1-5.0, P = .04]), but this did not correlate with any of the ns-SNPs. We conclude that immune responses to "sequence-mismatched" FVIII products are unlikely to contribute appreciably to the inhibitor incidence in African Americans.

  18. Danger signal-dependent activation of human dendritic cells by plasma-derived factor VIII products.

    Science.gov (United States)

    Miller, L; Weissmüller, S; Ringler, E; Crauwels, P; van Zandbergen, G; Seitz, R; Waibler, Z

    2015-08-01

    Treatment of haemophilia A by infusions of the clotting factor VIII (FVIII) results in the development of inhibitors/anti-drug antibodies in up to 25 % of patients. Mechanisms leading to immunogenicity of FVIII products are not yet fully understood. Amongst other factors, danger signals as elicited upon infection or surgery have been proposed to play a role. In the present study, we focused on effects of danger signals on maturation and activation of dendritic cells (DC) in the context of FVIII application. Human monocyte-derived DC were treated with FVIII alone, with a danger signal alone or a combination of both. By testing more than 60 different healthy donors, we show that FVIII and the bacterial danger signal lipopolysaccharide synergise in increasing DC activation, as characterised by increased expression of co-stimulatory molecules and secretion of pro-inflammatory cytokines. The degree and frequency of this synergistic activation correlate with CD86 expression levels on immature DC prior to stimulation. In our assay system, plasma-derived but not recombinant FVIII products activate human DC in a danger signal-dependent manner. Further tested danger signals, such as R848 also induced DC activation in combination with FVIII, albeit not in every tested donor. In our hands, human DC but not human B cells or macrophages could be activated by FVIII in a danger signal-dependent manner. Our results suggest that immunogenicity of FVIII is a result of multiple factors including the presence of danger, predisposition of the patient, and the choice of a FVIII product for treatment.

  19. Application of a novel affinity adsorbent for the capture and purification of recombinant factor VIII compounds.

    Science.gov (United States)

    McCue, Justin T; Selvitelli, Keith; Walker, Joshua

    2009-11-06

    Recombinant Factor VIII (FVIII) therapies have been created to provide treatment for Hemophilia A, an inherited bleeding disorder caused by mutation in the FVIII gene. A major challenge in the purification of recombinant FVIII molecules is the development of an affinity chromatography step. Such a step must be highly specific and selective for the FVIII molecule, but also must be designed appropriately to ensure the FVIII molecule can be effectively recovered without resorting to harsh elution conditions which may be harmful to the product. Additionally, it is desirable to have affinity adsorbents designed to be reusable over a large number of column cycles while maintaining consistent purification performance. In this work, we describe the use of VIIISelect, a commercially available affinity adsorbent designed specifically for the purification of FVIII compounds. The VIIISelect adsorbent consists of a 13kDa recombinant protein ligand attached to a cross-linked agarose base matrix. The structure of the recombinant ligand is based upon Camelid-derived single domain antibody fragments. The VIIISelect adsorbent is produced using a process free of animal-derived raw materials, which is a highly desirable attribute for adsorbents used in the purification processes of recombinant protein therapeutics. The VIIISelect adsorbent was used as the initial capture column to purify a FVIII compound directly from clarified cell culture fluid prior to further downstream purification. The purification performance of the VIIISelect was evaluated, which included measurement of the static binding capacity, dynamic binding capacity, product recovery, impurity clearance, and adsorbent reuse. Following laboratory-scale process development, the VIIISelect adsorbent was scaled up and used in the large scale manufacturing of a FVIII compound.

  20. Intraosseous delivery of lentiviral vectors targeting factor VIII expression in platelets corrects murine hemophilia A.

    Science.gov (United States)

    Wang, Xuefeng; Shin, Simon C; Chiang, Andy F J; Khan, Iram; Pan, Dao; Rawlings, David J; Miao, Carol H

    2015-04-01

    Intraosseous (IO) infusion of lentiviral vectors (LVs) for in situ gene transfer into bone marrow may avoid specific challenges posed by ex vivo gene delivery, including, in particular, the requirement of preconditioning. We utilized IO delivery of LVs encoding a GFP or factor VIII (FVIII) transgene directed by ubiquitous promoters (a MND or EF-1α-short element; M-GFP-LV, E-F8-LV) or a platelet-specific, glycoprotein-1bα promoter (G-GFP-LV, G-F8-LV). A single IO infusion of M-GFP-LV or G-GFP-LV achieved long-term and efficient GFP expression in Lineage(-)Sca1(+)c-Kit(+) hematopoietic stem cells and platelets, respectively. While E-F8-LV produced initially high-level FVIII expression, robust anti-FVIII immune responses eliminated functional FVIII in circulation. In contrast, IO delivery of G-F8-LV achieved long-term platelet-specific expression of FVIII, resulting in partial correction of hemophilia A. Furthermore, similar clinical benefit with G-F8-LV was achieved in animals with pre-existing anti-FVIII inhibitors. These findings further support platelets as an ideal FVIII delivery vehicle, as FVIII, stored in α-granules, is protected from neutralizing antibodies and, during bleeding, activated platelets locally excrete FVIII to promote clot formation. Overall, a single IO infusion of G-F8-LV was sufficient to correct hemophilia phenotype for long term, indicating that this approach may provide an effective means to permanently treat FVIII deficiency.

  1. Enhanced factor VIII heavy chain for gene therapy of hemophilia A.

    Science.gov (United States)

    Chen, Lingxia; Lu, Hui; Wang, Jinhui; Sarkar, Rita; Yang, Xiao; Wang, Hongli; High, Katherine A; Xiao, Weidong

    2009-03-01

    Hemophilia A gene therapy using recombinant adenovirus-associated virus (AAV) vectors has been hampered by the size of the factor VIII (FVIII) cDNA. Previously, splitting the FVIII coding sequence into a heavy-chain (HC) fragment and a light-chain (LC) fragment for dual recombinant AAV vector delivery has been successfully explored. However, the main disadvantage of this approach is a "chain imbalance" problem in which LC secretion is approximately 1-2 logs higher than that of HC, and therefore, the majority of protein synthesized is nonfunctional. To improve HC secretion, we constructed alternate FVIII HCs based on our observation that LC facilitates HC secretion. To our surprise, most of the new HC molecules exhibited enhanced expression over the traditional HC molecule (HC(745)). The optimized HC mutein, HC(HL), including additional acidic-region-3 (ar3) sequences, exhibited three- to fivefold higher activity in both enzyme-linked immunosorbent assay (ELISA) and activated partial thromboplastin time (aPTT) assay in in vitro testing. Further characterization suggested ar3 sequences increased HC secretion, rather than promoting HC synthesis. Intravenous delivery of AAV8-HC(HL)+AAV8-LC or AAV8-HC(745)+AAV8-LC achieved phenotypic correction in hemophilia A mice. Mice receiving AAV8-HC(HL)+AAV8-LC achieved three- to fourfold higher HC expression than AAV8-HC(745)+AAV8-LC, consistent with the FVIII functional assays. HC(HL) should be substituted for HC(745) in a dual AAV vector strategy due to its enhanced expression.

  2. The impact of von Willebrand factor on factor VIII memory immune responses.

    Science.gov (United States)

    Chen, Juan; Schroeder, Jocelyn A; Luo, Xiaofeng; Shi, Qizhen

    2017-08-22

    Immune tolerance induction (ITI) with aggressive infusion of factor VIII (FVIII) is the current strategy used to eradicate FVIII inhibitors and restore normal FVIII pharmacokinetics in inhibitor patients. Whether the use of FVIII products containing von Willebrand factor (VWF) will affect the efficacy of ITI is still controversial. In this study, we explored the impact of VWF on FVIII memory immune responses in hemophilia A (HA) mice. A T-cell proliferation assay and cytokine profile analysis were used to study FVIII-primed CD4(+) T cells. When CD4(+) T cells from primed FVIII(null) mice were restimulated with recombinant human FVIII (rhF8) plus recombinant human VWF (rhVWF) in vitro, the percentages of daughter CD4(+) T cells were significantly decreased compared with the groups cultured with rhF8 only. Levels of interferon-γ and interleukin 10 were significantly lower in the rhF8 plus rhVWF groups than in the rhF8 groups. When memory B-cell pools from primed FVIII(null) mice were cultured with rhF8 with or without rhVWF to induce differentiation of memory B cells into antibody-secreting cells (ASCs), the number of ASCs was significantly lower in the rhF8 plus VWF group than in the rhF8 group. When memory B-cell pools were transferred into NSGF8KO mice followed by rhF8 immunization with or without rhVWF, the titers of anti-F8 inhibitors and total immunoglobulin G were significantly higher in the rhF8 group than in the rhF8 plus rhVWF group, with an average difference of 2.23- and 2.04-fold. Together, our data demonstrate that VWF attenuates FVIII memory immune responses in HA mice.

  3. The factor VIII Structure and Mutation Resource Site: HAMSTeRS version 4.

    Science.gov (United States)

    Kemball-Cook, G; Tuddenham, E G; Wacey, A I

    1998-01-01

    Since 1996 the HAMSTeRS (Haemophilia A Mutation, Search, Test and Resource Site) WWW site has provided an online resource for access to data on the molecular pathology of haemophilia A, replacing previous text editions of the Haemophilia A Database published in Nucleic Acids Research . This report describes the continued development of the site (version 4), and in particular the expansion of factor VIII (FVIII) structure-related features. Access to the mutation database itself, both for searching the listings and for submission of new mutations, is via custom-designed forms: more powerful Boolean searches of the point mutations in the database are also available. During 1997 a total of 22 novel missense mutations were reported, increasing the total number of unique variants now described to 252 (238 in exonic sequences and 14 at intronic splice junctions). Currently, a total of 586 individual reports with associated phenotypic data are available for searching by any category including phenotype. The FVIII structure section now includes a download of a FVIII A domain homology model in Protein Data Bank format and a multiple alignment of the FVIII amino-acid sequencies from four species (human, murine, porcine and canine) in addition to the virtual reality simulations, secondary structural data and FVIII animation already available. Finally, to aid navigation across this site, a clickable roadmap of the main features provides easy access to the page desired. Our intention is that continued development and updating of the site shall provide workers in the fields of molecular and structural biology with a one-stop resource site to facilitate FVIII research and education. The HAMSTeRS URL is http://europium.mrc.rpms.ac.uk

  4. Frequencies of VNTR and RFLP polymorphisms associated with factor VIII gene in Singapore

    Energy Technology Data Exchange (ETDEWEB)

    Fong, I.; Lai, P.S.; Ouah, T.C. [National Univ. of Singapore (Malaysia)] [and others

    1994-09-01

    The allelic frequency of any polymorphism within a population determines its usefulness for genetic counselling. This is important in populations of non-Caucasian origin as RFLPs may significantly differ among ethnic groups. We report a study of five intragenic polymorphisms in factor VIII gene carried out in Singapore. The three PCR-based RFLP markers studied were Intron 18/Bcl I, Intron 19/Hind III and Intron 22/Xba I. In an analysis of 148 unrelated normal X chromosomes, the allele frequencies were found to be A1 = 0.18, A2 = 0.82 (Bcl I RFLP), A1 = 0.80, A2 = 0.20 (Hind III RFLP) and A1 = 0.58, and A2 = 0.42 (Xba I RFLP). The heterozygosity rates of 74 females analyzed separately were 31%, 32% and 84.2%, respectively. Linkage disequilibrium was also observed to some degree between Bcl I and Hind III polymorphism in our population. We have also analyzed a sequence polymorphism in Intron 7 using hybridization with radioactive-labelled {sup 32}P allele-specific oligonucleotide probes. This polymorphism was not very polymorphic in our population with only 2% of 117 individuals analyzed being informative. However, the use of a hypervariable dinucleotide repeat sequence (VNTR) in Intron 13 showed that 25 of our of 27 (93%) females were heterozygous. Allele frequencies ranged from 1 to 55 %. We conclude that a viable strategy for molecular analysis of Hemophilia A families in our population should include the use of Intron 18/Bcl I and Intron 22/Xba I RFLP markers and the Intron 13 VNTR marker.

  5. Pharmacokinetic properties of BAY 81-8973, a full-length recombinant factor VIII.

    Science.gov (United States)

    Shah, A; Delesen, H; Garger, S; Lalezari, S

    2015-11-01

    BAY 81-8973 is a full-length recombinant factor VIII (FVIII) with the same primary amino acid sequence as sucrose-formulated recombinant FVIII (rFVIII-FS) but is produced with advanced manufacturing technologies. To analyse the pharmacokinetics (PK) of BAY 81-8973 after single and multiple dosing across different age and ethnic groups in the LEOPOLD clinical trial programme. The LEOPOLD trials enrolled patients with severe haemophilia A aged 12-65 years (LEOPOLD I and II) or ≤12 years (LEOPOLD Kids) with ≥150 (LEOPOLD I and II) or ≥50 (LEOPOLD Kids) exposure days to any FVIII product and no history of FVIII inhibitors. PK were assessed using chromogenic and one-stage assays (only chromogenic assay for LEOPOLD Kids) after a single 50-IU kg(-1) dose of BAY 81-8973 and, in a subset of patients in LEOPOLD I, after repeated dosing. Pharmacokinetic analyses were also performed based on age (18 to 65, 12 to <18, 6 to <12 and <6 years) and ethnicity (Asian and non-Asian). Pharmacokinetic assessments in the LEOPOLD I trial showed non-inferiority of BAY 81-8973 vs. rFVIII-FS. The PK of BAY 81-8973 were comparable after single and multiple dosing. Age-based analysis in the three trials showed that plasma concentrations were slightly lower for children, but similar for adolescents compared with adults. Pharmacokinetic results were similar in the different ethnic groups. Results of the LEOPOLD trials show that the BAY 81-8973 pharmacokinetic profile is non-inferior to rFVIII-FS. Similar BAY 81-8973 pharmacokinetic values were observed following single and repeated dosing and across ethnic groups. © 2015 John Wiley & Sons Ltd.

  6. RRS "Charles Darwin" Cruise 150, 22 Aug - 15 Sep 2003. Benthic ecology and biogeochemistry of the Pakistan Margin

    OpenAIRE

    B. J. Bett

    2004-01-01

    RRS Charles Darwin cruise 150 forms part of a larger programme of research (“Benthic processes in the Arabian Sea: interrelationships between benthos, sediment, biogeochemistry and organic matter cycling”, NER/A/S/2000/01280), focusing on the benthic biogeochemistry of the Pakistan Margin, that includes four cruises in total (CD145, 146, 150 and 151). The primary objectives of the present cruise were: a) to revisit a series of five previously established study sites (A140, A300, A950, A1200 a...

  7. MENINGKATKAN AKTIVITAS BELAJAR SISWA DENGAN MENGGUNAKAN METODE GROUP INVESTIGATION (GI PADA POKOK BAHASAN PASAR DI KELAS VIII SMP 5 KUDUS

    Directory of Open Access Journals (Sweden)

    Mutthofiyah Mutthofiyah

    2016-03-01

    Full Text Available Penelitian ini bertujuan untukmeningkatkan aktivitas belajar siswa dengan menggunakan metode group investigation (GI pada pokok bahasan pasar pada penelitian ini menggunakan penelitian tindakan kelas (PTK. Dalam penelitian ini dirancang menggunakan siklus, masing-masing siklus dengan tahapan perencanaan, pelaksanaan tindakan, observasi dan refleksi. Teknik pengumpulan data pada penelitian ini adalah dokumentasi, observasi dan tes.Hasil penelitian menunjukkan melalui meningkatkan metode group investigation (GI aktivitas siswa SMP 5 Kudus ada peningkatan yang sigifikan, dari siklus I kelas VIII F 15,8 (41,62% meningkat di siklus II menjadi 24,8 (72,9%, sedangkan di kelas VIII G siklus I 18,5 (47,04% meningkat menjadi 26,2 (74,7%; melalui penerapan metode group investigation hasil belajar siswa SMP 5 kudus meningkat sangat signifkan, ketuntasan belajar secara klasikal dari kelas VIII F pra siklus sebesar 17,64% terjadi peningkatan 47% ke siklus I dan meningkat menjadi 82,35% di siklus II. Sedangkan di kelas VII G pra siklus sebesar 22,85% meningkat 54,28% di siklus I dan meningkat menjadi 94,28% di siklus II. Saran yang peneliti berikan adalah sebagai berikut:1Kepada para guru diharapkan dapat menerapkan pembelajaran kooperatif khususnya pembelajaran kooperatif tipe Group Investigation (GI dalam proses pembelajaran ekonomi dan mata pelajaran lainya dan mengembangkannya; 2 Kepada para peneliti selanjutnya diharapkan dapat melakukan penelitian tentang penerapan model pembelajaran yang dapat membangkitkan keaktifan siswa untuk belajar ekonomi. This study aims to improve students' learning activities using group investigation (GI on the subject of markets in class VIII SMP 5 Kudus. In this study is designed to use cycles, with each cycle stages of planning, action, observation and reflection. Data collection techniques in this study is documentation, observation and tests. Results showed through improved methods of group investigation (GI junior 5 high

  8. Expression, purification, and partial in vitro characterization of biologically active human coagulation factor VIII light chain (A3-C1-C2) in Pichia pastoris.

    Science.gov (United States)

    A R, Sudheer Reddy; Satheeshkumar, Padikara Kutty; Vijayalakshmi, Mookambeswaran A

    2013-09-01

    Recombinant coagulation factor VIII (FVIII) expressed in mammalian expression systems is used extensively in the treatment of hemophilia A. It is reported that the heavy (A1-A2) and light chains (A3-C1-C2) of factor VIII purified from plasma regained the coagulation activity by dimerization in vitro. In this work, cDNA coding for the light chain of human coagulation factor VIII (FVIII-LC) was cloned into pPICZα-A expression vector downstream of alcohol oxidase promoter and α-mating signal sequence from Saccharomyces cerevisiae in order to express the protein with a native N-terminus. The methylotrophic yeast, Pichia pastoris X-33, was transformed with this cassette, and transformants were selected for production of human factor VIII light chain into culture media. SDS-PAGE and Western blot analysis confirmed the expression of factor VIII light chain protein. The expressed protein was purified to near homogeneity using histidine ligand affinity chromatography (2.342 mg/L). The biological activity of FVIII-LC was confirmed by analyzing the interaction between FVIII-LC and phospholipid vesicles. The data presented here indicate the possibilities of exploring cost-effective systems to express complex proteins of therapeutic value.

  9. Successful immune tolerance induction consisting of high-dose factor VIII rich in von Willebrand factor and pulsed intravenous immunoglobulin: a case report

    Directory of Open Access Journals (Sweden)

    Kubisz Peter

    2012-10-01

    Full Text Available Abstract Introduction The development of factor VIII inhibitors is a serious complication of replacement therapy in patients with congenital hemophilia A. Immune tolerance induction has been accepted as the only clinically proven treatment allowing antigen-specific tolerance to factor VIII. However, some of its issues, such as patient selection, timing, factor VIII dosing, use of immunosuppressive or immunomodulatory procedures, still remain the subject of debate. Case presentation A case of a 3-year-old Caucasian boy with severe congenital hemophilia A, intron 22 inversion of the F8 gene and high-titer inhibitor, who underwent an immune tolerance induction according to the modified Bonn regimen (high doses of plasma-derived factor VIII rich in von Willebrand factor and pulsed intravenous immunoglobulin is presented. The treatment lasted for 13 months and led to the eradication of inhibitor. Conclusion Addition of intravenous immunoglobulin did not negatively affect the course of immune tolerance induction and led to the rapid eradication of factor VIII inhibitor.

  10. Therapeutic and routine prophylactic properties of rFactor VIII Fc (efraloctocog alfa, Eloctate®) in hemophilia A

    Science.gov (United States)

    Chowdary, Pratima; Fosbury, Emma; Riddell, Anne; Mathias, Mary

    2016-01-01

    rFVIIIFc (efraloctocog alfa, Eloctate®) is an extended half-life (EHL) factor VIII licensed for use in patients with hemophilia A for prophylaxis and treatment of bleeding and surgical episodes. Pharmacokinetic studies in adults have shown a mean 1.5-fold increase in half-life compared to full-length factor VIII. When compared to adults, the half-life is decreased by 8% in adolescents between 12 and 17 years, by 18% in children 6 to <12 years, and by 33% in children between the ages of 2 and <6 years. There is a considerable interindividual variation in the prolongation of the half-life particularly in children and across the age groups, the range extending from no increase to a 2.5-fold increase. In addition to age, von willebrand factor (VWF) antigen level has demonstrated a significant impact on rFVIIIFc half-life, with higher VWF levels associated with greater prolongation of half-life. The pivotal and pediatric clinical trials have demonstrated the efficacy and safety of rFVIIIFc for use in regular prophylaxis and in management of bleeds and surgery. In these studies, just under half the participants showed a zero annualized bleed rate (ABR), and the median ABR (1.6 in the pivotal study for the individualized prophylaxis arm) showed a further decrease in the extension study. On average, the patients required fewer infusions (reduced by at least a third), and the mean weekly consumption seems to be in keeping with standard recombinant factor VIII. EHL rFVIIIFc has made decreased infusion frequency a possibility. However, the interindividual variability in dose and infusion frequency highlights the need for a personalized approach based on individual patient’s half-life and/or response to treatment. PMID:27695377

  11. Seleucid, Demotic and Mediterranean mathematics versus Chapters VIII and IX of the Nine Chapters: accidental or significant similarities?

    DEFF Research Database (Denmark)

    Høyrup, Jens

    2016-01-01

    Similarities of geometrical diagrams and arithmetical structures of problems have often been taken as evidence of transmission of mathematical knowledge or techniques between China and “the West”. Confronting on one hand some problems from Chapter VIII of the Nine Chapters with comparable problems...... known from Ancient Greek sources, on the other a Seleucid collection of problems about rectangles with a subset of the triangle problems from Chapter IX, it is concluded, (1) that transmission of some arithmetical riddles without method – not “from Greece” but from a transnational community of traders...

  12. DAMPAK POSITIF DAN NEGATIF SOSIAL MEDIA TERHADAP PENDIDIKAN AKHLAK ANAK (Studi Kasus di SMP Negeri 2 Kelas VIII Banda Aceh)

    OpenAIRE

    NISA KHAIRUNI

    2016-01-01

    The author examines the positive and negative effects of social media on the moral education of children in school SMPN 2 class VIII Banda Aceh, it is very interesting because of the many children who abuse social media. This work analyzes about the use of social media that have an impact on the moral education of children. The data collected in this study through the work of other literature, researchers also interviewed principals and students as many as 16 students as well as the observati...

  13. EK(b)P Keskkomitee VIII pleenumi stenogramm / tõlk. Mart Arold, tõlk. Jaan Isotamm

    Index Scriptorium Estoniae

    1999-01-01

    EK(b)P Keskkomitee VIII pleenum, 21.-23. märts 1950. Sõnavõtud: N. Karotamm, P. Ponomarenko, I. Käbin, V. Kuusik, A. Jaanus, V. Hiznjakov, E. Ristimägi, I. Suija, H. Ajo, A. Meri, G. Abels, M. Assin, G. Latõshov, Rumjantsev, Müürisepp. Kommenteerinud Viktor Niitsoo. Algus: Akadeemia (1998) nr. 12, lk. 2655-2683 ; Järg nr.2;3;4;5;6;7;8;9;10 lk.415-446;639-670;863-894;1087-1118;1310-1342;1534-1566;1759-1790;2017-2048;2220-2256

  14. Changes of androgen receptor expression in stages VII-VIII seminiferous tubules of rat testis after exposure to methamphetamine

    OpenAIRE

    Sutita Nudmamud-Thanoi; Nareelak Tangsrisakda; Samur Thanoi

    2016-01-01

    Methamphetamine (METH) is toxic not only to the central nervous system but also to the reproductive system. This study aimed to investigate the effect of METH administration on androgen receptor (AR) expression in the seminiferous tubules (stages VII-VIII) and Leydig cells. 24 Sprague-Dawley rats were equally divided into 4 groups; control, acute doseMETH binge (AB-METH), escalating dose-METH (ED-METH) and escalating dose-METH binge (ED-METH binge) groups. The expressions of AR we...

  15. CONSTRAINING THE MILKY WAY'S HOT GAS HALO WITH O VII AND O VIII EMISSION LINES

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Matthew J.; Bregman, Joel N., E-mail: mjmil@umich.edu, E-mail: jbregman@umich.edu [Department of Astronomy, University of Michigan, Ann Arbor, MI 48104 (United States)

    2015-02-10

    The Milky Way hosts a hot (≈2 × 10{sup 6} K), diffuse, gaseous halo based on detections of z = 0 O VII and O VIII absorption lines in quasar spectra and emission lines in blank-sky spectra. Here we improve constraints on the structure of the hot gas halo by fitting a radial model to a much larger sample of O VII and O VIII emission line measurements from XMM-Newton/EPIC-MOS spectra compared to previous studies (≈650 sightlines). We assume a modified β-model for the halo density distribution and a constant-density Local Bubble from which we calculate emission to compare with the observations. We find an acceptable fit to the O VIII emission line observations with χ{sub red}{sup 2} (dof) = 1.08 (644) for best-fit parameters of n{sub o}r{sub c}{sup 3β}=1.35±0.24 cm{sup –3} kpc{sup 3β} and β = 0.50 ± 0.03 for the hot gas halo and negligible Local Bubble contribution. The O VII observations yield an unacceptable χ{sub red}{sup 2} (dof) = 4.69 (645) for similar best-fit parameters, which is likely due to temperature or density variations in the Local Bubble. The O VIII fitting results imply hot gas masses of M(<50 kpc) = 3.8{sub −0.3}{sup +0.3}×10{sup 9} M{sub ⊙} and M(<250 kpc) = 4.3{sub −0.8}{sup +0.9}×10{sup 10} M{sub ⊙}, accounting for ≲50% of the Milky Way's missing baryons. We also explore our results in the context of optical depth effects in the halo gas, the halo gas cooling properties, temperature and entropy gradients in the halo gas, and the gas metallicity distribution. The combination of absorption and emission line analyses implies a sub-solar gas metallicity that decreases with radius, but that also must be ≥0.3 Z {sub ☉} to be consistent with the pulsar dispersion measure toward the Large Magellanic Cloud.

  16. EK(b)P Keskkomitee VIII pleenumi stenogramm / tõlk. Mart Arold, tõlk. Jaan Isotamm

    Index Scriptorium Estoniae

    1999-01-01

    EK(b)P Keskkomitee VIII pleenum, 21.-23. märts 1950. Sõnavõtud: N. Karotamm, P. Ponomarenko, I. Käbin, V. Kuusik, A. Jaanus, V. Hiznjakov, E. Ristimägi, I. Suija, H. Ajo, A. Meri, G. Abels, M. Assin, G. Latõshov, Rumjantsev, Müürisepp. Kommenteerinud Viktor Niitsoo. Algus: Akadeemia (1998) nr. 12, lk. 2655-2683 ; Järg nr.2;3;4;5;6;7;8;9;10 lk.415-446;639-670;863-894;1087-1118;1310-1342;1534-1566;1759-1790;2017-2048;2220-2256

  17. Love and/in psychoanalysis: a commentary on Lacan's reading of Plato's Symposium in Seminar VIII: Transference.

    Science.gov (United States)

    Fink, Bruce

    2015-02-01

    What is love and what part does it play in psychoanalysis? Where are the analyst and the analysand situated in relation to the roles defined as those of the "lover" and the "beloved"? Jacques Lacan explores these and other questions in his soon-to-be-published Seminar VIII: Transference by providing an extensive commentary on Plato's most famous dialogue on love, the Symposium. This paper outlines some of the major points about love that grow out of Lacan's reading of the dialogue and examines their relevance to the analytic setting. Can the analyst be characterized as a sort of modern-day Socrates?

  18. Conversaciones en el VIII Congreso Panamericano de Mecánica de Suelos e Ingeniería de Fundaciones

    OpenAIRE

    Ruiz Restrepo, Carlos Enrique

    1988-01-01

    En el mes de agosto de 1987 se cumplió, en Cartagena de Indias el "VIII Congreso Panamericano de Mecánica de Suelos", bajo la presidencia del geólogo colombiano Juan Montero Olarte, asistido por un Comité Organizador de todas las excelencias. Abrir canales para la comunicación permanente entre todos nuestros centros educativos, o entre los más representativos, es el método más simple para garantizar, como en simbólico vaso comunicante, que el nivel de enseñanza vibre en un clima de con...

  19. Functional characterisation of a metagenome derived family VIII esterase with a deacetylation activity on ß-lactam antibiotics

    CSIR Research Space (South Africa)

    Mokoena, N

    2013-08-01

    Full Text Available of a metagenome derived family VIII esterase with deacetylation activity on β-lactam antibiotics Nobalanda Mokoena1, Kgama Mathiba1, Tsepo Tsekoa1, Paul Steenkamp1,2 and Konanani Rashamuse1* 1CSIR Biosciences, Scientia campus, PO Box 395... of 0.3 U/L and the antibiotic (50 µg/mL Kanamycin). The culture was incubated at 16°C overnight and then booster tablet and additional enzyme mixture (0.6 U/L) were added to the culture. Est22 protein expression was induced with ITPG (1mM) followed...

  20. [The beneficial effect of mineral waters of Fiuggi on Pope Bonifacio VIII and Michelangelo: two prominent calculous patients].

    Science.gov (United States)

    Androutsos, Georges

    2005-09-01

    Going back to the dawn of time, we deal with two historical figures, that of Pope Bonifacio VIII, and that of the hierophant of art, Michelangelo Buonarroti. The texts and the codes of the British Museum (Department of Manuscripts), of the Biblioteca Medica Laurenziana (Archivio Buonarroti) of Florence, and the Biblioteca Apostolica of Vatican, convincing detalled evidence that both figures were urological patients. It concerns two clinical cases with especially pertain to the history of lithiasis of urinary tract. Even though, both of them--the first with nephrolithiasis or gravel, and the second with a bladder stone--were historically bound with the well-known cures of Fiuggi in Italy.

  1. Archive of Datasonics SIS-1000 Boomer Subbottom Data Collected During USGS Cruise DIAN 97011 Long Island, NY Inner Shelf

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This CD-ROM contains digital high resolution seismic reflection data collected during the USGS DIAN 97011 cruise. The coverage is the nearshore of Long Island, NY in...

  2. AFSC/RACE/EcoFOCI: 2011 Gulf of Alaska fall juvenile fish Cruise DY11-06/7DY11

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The cruise began when the ship departed Dutch Harbor on October 1, 2011 at 1500 ADT. Sampling commenced at collection site 1E, which corresponds to Station 1....

  3. Archive of Water Gun Subbottom Data Collected During USGS Cruise SEAX 96004, New York Bight, 1 May - 9 June, 1996

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This DVD-ROM contains digital high resolution seismic reflection data collected during the USGS SEAX 96004 cruise. The coverage is the nearshore of the New York and...

  4. Archive of Datasonics SIS-1000 Boomer Subbottom Data Collected During USGS Cruise DIAN 97011 Long Island, NY Inner Shelf

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This CD-ROM contains digital high resolution seismic reflection data collected during the USGS DIAN 97011 cruise. The coverage is the nearshore of Long Island, NY in...

  5. Archive of Boomer Subbottom Data Collected During USGS Cruise DIAN 96040, Fire Island, New York, 4-24 September 1996

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This CD-ROM contains digital high resolution seismic reflection data collected during the USGS DIAN 97011 cruise. The coverage is the nearshore of Long Island, NY in...

  6. A fuel-efficient cruise performance model for general aviation piston engine airplanes. Ph.D. Thesis. Final Report

    Science.gov (United States)

    Parkinson, R. C. H.

    1983-01-01

    A fuel-efficient cruise performance model which facilitates maximizing the specific range of General Aviation airplanes powered by spark-ignition piston engines and propellers is presented. Airplanes of fixed design only are considered. The uses and limitations of typical Pilot Operating Handbook cruise performance data, for constructing cruise performance models suitable for maximizing specific range, are first examined. These data are found to be inadequate for constructing such models. A new model of General Aviation piston-prop airplane cruise performance is then developed. This model consists of two subsystem models: the airframe-propeller-atmosphere subsystem model; and the engine-atmosphere subsystem model. The new model facilitates maximizing specific range; and by virtue of its implicity and low volume data storge requirements, appears suitable for airborne microprocessor implementation.

  7. Dive Activities from Cruise Information Management System (CIMS) for Operation Deep Scope 2005 - Office of Ocean Exploration

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Information about dive activities were recorded into the Cruise Information Management System (CIMS) by the NOAA Office of Ocean Exploration's data manager during...

  8. Dive Activities from Cruise Information Management System (CIMS) for Life on the Edge 2005 - Office of Ocean Exploration

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Information about dive activities were recorded into the Cruise Information Management System (CIMS) by the NOAA Office of Ocean Exploration's data manager during...

  9. Dive Activities from Cruise Information Management System (CIMS) for Life on the Edge 2004 - Office of Ocean Exploration

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Information about dive activities were recorded into the Cruise Information Management System (CIMS) by the NOAA Office of Ocean Exploration's data manager during...

  10. Dive Activities from Cruise Information Management System (CIMS) for Investigating the Charleston Bump 2003 - Office of Ocean Exploration

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Information about dive activities were recorded into the Cruise Information Management System (CIMS) by the NOAA Office of Ocean Exploration's data manager during...

  11. Archive of Boomer Subbottom Data Collected During USGS Cruise DIAN 96040, Fire Island, New York, 4-24 September 1996

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This CD-ROM contains digital high resolution seismic reflection data collected during the USGS DIAN 97011 cruise. The coverage is the nearshore of Long Island, NY in...

  12. Archive of Boomer Subbottom Data Collected During USGS Cruise SEAX 96004, New York Bight, 1 May - 9 June, 1996

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This DVD-ROM contains digital high resolution seismic reflection data collected during the USGS SEAX 96004 cruise.The coverage is the nearshore of the New York and...

  13. Archive of Boomer Subbottom Data Collected During USGS Cruise SEAX 96004, New York Bight, 1 May - 9 June, 1996

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This DVD-ROM contains digital high resolution seismic reflection data collected during the USGS SEAX 96004 cruise.The coverage is the nearshore of the New York and...

  14. Archive of Boomer Subbottom Data Collected During USGS Cruise DIAN 96040, Fire Island, New York, 4-24 September 1996

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This CD-ROM contains digital high resolution seismic reflection data collected during the USGS DIAN 97011 cruise. The coverage is the nearshore of Long Island, NY...

  15. Archive of Datasonics SIS-1000 Boomer Subbottom Data Collected During USGS Cruise DIAN 97011 Long Island, NY Inner Shelf

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This CD-ROM contains digital high resolution seismic reflection data collected during the USGS DIAN 97011 cruise. The coverage is the nearshore of Long Island, NY...

  16. Archive of Water Gun Subbottom Data Collected During USGS Cruise SEAX 96004, New York Bight, 1 May - 9 June, 1996

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This DVD-ROM contains digital high resolution seismic reflection data collected during the USGS SEAX 96004 cruise. The coverage is the nearshore of the New York and...

  17. Larval Fish and Midwater Trawl Fish Identification from Cruises to Palmyra, TC-90-07 and TC-92-01

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Two cruises aboard the NOAA ship Townsend Cromwell were conducted during the following periods: 22 August-17 September 1990 and 18 February-9 March, 1992. Collectors...

  18. A summary of Alaska's unique cruise ship program : wastewater, air emissions, and ocean rangers

    Energy Technology Data Exchange (ETDEWEB)

    Koch, D. [Alaska Dept. of Environmental Conservation, Juneau, AK (United States)

    2009-07-01

    Increased environmental awareness has led to concerns over the impacts of cruise ships on Alaska's marine environment. Federal legislation has been passed to ensure that large cruise ships no longer dump bilge water in areas within 3 nautical miles from the state's shoreline. The state has also been legislation to regulate sewage releases from both small and large vessels. The state requires registration, fees, and plans for emissions, and hazardous and solid wastes. As a result of the regulations, all large cruise ships discharging wastewater in Alaska had advanced wastewater treatment systems by 2003. The systems consist of solids separation, enhanced aerobic digestion, ultrafiltration, reverse osmosis, and ultraviolet disinfection systems. The quality of sewage dramatically improved in the region. Ocean rangers are now inspecting approximately 88 per cent of cruise ships visiting the Alaska region. Details of recent wastewater compliance actions were presented, as well as data on wastewater and waste emission limits. tabs., figs.

  19. RRS "Charles Darwin" Cruise CD168, 02 Feb - 16 Feb 2005. Submarine landslides around the Cape Verde Islands

    OpenAIRE

    Masson, D. G.

    2005-01-01

    Catastrophic large-scale landslides are a fundamental process in the formation of many oceanic islands. The main aim of cruise CD168 was to carry out a reconnaissance survey of the influence of landsliding on the Cape Verde islands, in the eastern central Atlantic, and to compare this with the known effects of landsliding on the Canary Islands. The data collected during RRS Charles Darwin Cruise, when combined with synthetic aperture radar imagery of the subaerial islands, show clear evidence...

  20. A Trans-Indian Ocean Hydrographic Section at Latitude 32 deg S Data Report of RRS Charles Darwin Cruise 29

    Science.gov (United States)

    1992-01-01

    Data Report of RRS Charles Darwin Cruise #29 by Margaret F. Cook, John M. Toole, and George P. Knapp Woods Hole Oceanographic Institution Woods Hole...Report of RRS Charles Darwin Cruise #29 by Margaret F. Cook, John M. Toole, and George P. Knapp Woods Hole Oceanographic Institution Woods Hole... Charles Darwin was a multi-institution oceanographic effort. A U.S. contingent of thirteen joined by four shipboard technicians from NERC/RVS (Table

  1. RRS "Discovery" Cruise 282, 30 Jun - 01 Aug 2003. The environment and ecology of Seine and Sedlo Seamounts, NE Atlantic

    OpenAIRE

    B. J. Bett

    2004-01-01

    The general aim of the cruise is to undertake a range of physical, chemical and biological investigations on and around Seine and Sedlo Seamounts. Specific objectives for the cruise included: a) the recovery of two current meter moorings from Seine Seamount (originally deployed from FS Poseidon in March 2004); b) to make underway observations of upper water column currents and zooplankton migrations (using ADCPs and 10 kHz echosounder); c) to assess water column hydrography, primary productio...

  2. Stability of adaptive cruise control systems taking account of vehicle response time and delay

    Energy Technology Data Exchange (ETDEWEB)

    Davis, L.C., E-mail: ldavis7@mailaps.org [10244 Normandy Dr., Plymouth, MI 48170 (United States)

    2012-08-20

    The region of string stability of a platoon of adaptive cruise control vehicles, taking into account the delay and response of the vehicle powertrain, is found. An upper bound on the explicit delay time as a function the first-order powertrain response time constant is determined. The system is characterized by a headway time constant, a sensitivity parameter, relative (to the vehicle immediately in front) velocity control, and delayed-velocity feedback or acceleration feedback. -- Highlights: ► I find the region of stability for a realistic adaptive cruise control system. ► Vehicle response time and explicit delay are included in the analysis. ► Delayed-feedback enlarges the parameter space that gives string stability.

  3. 3D-Route Planning Method to Realize Missile Cruise Flight

    Institute of Scientific and Technical Information of China (English)

    WANG Zheng-jie; FAN Chen; ZHU Qun-Min; TANG Wei

    2007-01-01

    In order to increase the aircraft's survival in the flight mission, it is necessary to carry out flight mission planning, which includes TF/TA\\+2(terrain following/terrain avoidance/threat avoidance).An approach to 3D-route planning based on A* heuristic search algorithm was selected to determine the routes of fiber optic guidance missile's cruise segment.The cost function was discussed, which was mainly related to the physical obstacle, threat exposure, and aircraft performance characteristics.The digital map techniques were presented, which included setting "no-go" area according to fiber's safety requirement.The optimal or the sub-optimal route was obtained, while the cost function constraints were satisfied and the stored terrain obtained from a real terrain was digitized.The algorithm is validated through simulation and can fulfill the route planning task which focuses on the cruise segment of fiber optic guidance missile.

  4. Cruise ships as a source of avian mortality during fall migration

    Science.gov (United States)

    Bocetti, C.I.

    2011-01-01

    Avian mortality during fall migration has been studied at many anthropogenic structures, most of which share the common feature of bright lighting. An additional, unstudied source of avian mortality during fall migration is recreational cruise ships that are brightly lit throughout the night. I documented a single mortality event of eight Common Yellowthroats (Geothlypis trichas) on one ship during part of one night in fall 2003, but suggest this is a more wide-spread phenomenon. The advertised number of ship-nights for 50 cruise ships in the Caribbean Sea during fall migration in 2003 was 2,981. This may pose a significant, additional, anthropogenic source of mortality that warrants further investigation, particularly because impacts could be minimized if this source of avian mortality is recognized. ?? 2011 by the Wilson Ornithological Society.

  5. Cruise observation and numerical modeling of turbulent mixing in the Pearl River estuary in summer

    Science.gov (United States)

    Pan, Jiayi; Gu, Yanzhen

    2016-06-01

    The turbulent mixing in the Pearl River estuary and plume area is analyzed by using cruise data and simulation results of the Regional Ocean Model System (ROMS). The cruise observations reveal that strong mixing appeared in the bottom layer on larger ebb in the estuary. Modeling simulations are consistent with the observation results, and suggest that inside the estuary and in the near-shore water, the mixing is stronger on ebb than on flood. The mixing generation mechanism analysis based on modeling data reveals that bottom stress is responsible for the generation of turbulence in the estuary, for the re-circulating plume area, internal shear instability plays an important role in the mixing, and wind may induce the surface mixing in the plume far-field. The estuary mixing is controlled by the tidal strength, and in the re-circulating plume bulge, the wind stirring may reinforce the internal shear instability mixing.

  6. Visual Assessment on Coastal Cruise Tourism: A Preliminary Planning Using Importance Performance Analysis

    Science.gov (United States)

    Trisutomo, S.

    2017-07-01

    Importance-Performance Analysis (IPA) has been widely applied in many cases. In this research, IPA was applied to measure perceive on coastal tourism objects and its possibility to be developed as coastal cruise tourism in Makassar. Three objects, i.e. Akkarena recreational site, Losari public space at waterfront, and Paotere traditional Phinisi ships port, were selected and assessed visually from water area by a group of purposive resource persons. The importance and performance of 10 attributes of each site were scored using Likert scale from 1 to 5. Data were processed by SPSS-21 than resulted Cartesian graph which the scores were divided in four quadrants: Quadrant I concentric here, Quadrant II keep up the good work, Quadrant III low priority, and Quadrant IV possible overkill. The attributes in each quadrant could be considered as the platform for preliminary planning of coastal cruise tour in Makassar

  7. A review of underwater bio-mimetic propulsion: cruise and fast-start

    Science.gov (United States)

    Chao, Li-Ming; Cao, Yong-Hui; Pan, Guang

    2017-08-01

    This paper reviews recent developments in the understanding of underwater bio-mimetic propulsion. Two impressive models of underwater propulsion are considered: cruise and fast-start. First, we introduce the progression of bio-mimetic propulsion, especially underwater propulsion, where some primary conceptions are touched upon. Second, the understanding of flapping foils, considered as one of the most efficient cruise styles of aquatic animals, is introduced, where the effect of kinematics and the shape and flexibility of foils on generating thrust are elucidated respectively. Fast-start propulsion is always exhibited when predator behaviour occurs, and we provide an explicit introduction of corresponding zoological experiments and numerical simulations. We also provide some predictions about underwater bio-mimetic propulsion.

  8. Multidisciplinary Design Optimization of a Morphing Wingtip Concept with Multiple Morphing Stages at Cruise

    Science.gov (United States)

    Leahy, Michael

    Morphing an aircraft wingtip can provide substantial performance improvement. Most civil transport aircraft are optimized for range but for other flight conditions such as take-off and climb they are used as constraints. These constraints could potentially reduce the performance of an aircraft at cruise. By altering the shape of the wingtip, we can force the load distribution to adapt to the required flight condition to improve performance. Using a Variable Geometry Truss Mechanism (VGTM) concept to morph the wingtip of an aircraft with a Multidisciplinary Design Optimization (MDO) framework, the current work will attempt to find an optimal wing and wingtip shape to minimize fuel consumption for multiple morphing stages during cruise. This optimization routine was conducted with a Particle Swarm Optimization (PSO) algorithm using different fidelity tools to analyze the aerodynamic and structural disciplines.

  9. Influenza B virus outbreak on a cruise ship--Northern Europe, 2000.

    Science.gov (United States)

    2001-03-02

    During June 23-July 5, 2000, an outbreak of respiratory illnesses occurred on the MS Rotterdam (Holland America Line & Windstar Cruises) during a 12-day Baltic cruise from the United Kingdom to Germany via Russia. The ship carried 1311 passengers, primarily from the United States, and 506 crew members from many countries. Although results of rapid viral testing for influenza A and B viruses were negative, immunofluorescence staining and viral culture results implicated influenza B virus infection as the cause of the outbreak. This report summarizes the findings of the outbreak investigation conducted by the ship's medical department and describes the measures taken to control the outbreak. Travelers at high risk for complications of influenza who were not vaccinated with influenza vaccine during the preceding fall or winter should consider receiving influenza vaccine before travel with large tourist groups at any time of year or to certain regions of the world.

  10. Acute gastroenteritis and video camera surveillance: a cruise ship case report.

    Science.gov (United States)

    Diskin, Arthur L; Caro, Gina M; Dahl, Eilif

    2014-01-01

    A 'faecal accident' was discovered in front of a passenger cabin of a cruise ship. After proper cleaning of the area the passenger was approached, but denied having any gastrointestinal symptoms. However, when confronted with surveillance camera evidence, she admitted having the accident and even bringing the towel stained with diarrhoea back to the pool towels bin. She was isolated until the next port where she was disembarked. Acute gastroenteritis (AGE) caused by Norovirus is very contagious and easily transmitted from person to person on cruise ships. The main purpose of isolation is to avoid public vomiting and faecal accidents. To quickly identify and isolate contagious passengers and crew and ensure their compliance are key elements in outbreak prevention and control, but this is difficult if ill persons deny symptoms. All passenger ships visiting US ports now have surveillance video cameras, which under certain circumstances can assist in finding potential index cases for AGE outbreaks.

  11. Synthesis of dispersive iron or iron–silver nanoparticles on engineered capsid pVIII of M13 virus with electronegative terminal peptides

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Shuai; Nakano, Kazuhiko; Zhang, Shu-liang; Yu, Hui-min, E-mail: yuhm@tsinghua.edu.cn [Tsinghua University, Key Laboratory of Industrial Biocatalysis of the Ministry of Education, Department of Chemical Engineering (China)

    2015-10-15

    M13 is a filamentous Escherichia coli virus covered with five types of capsid proteins, in which pVIII with ∼2700 copies was around the cylindered surface and pIII with five copies located at one end of the phage particle. The pIII-engineered M13 phages with enhanced binding specificity toward Fe were screened after five rounds of biopanning, and the one containing ATPTVAMSLSPL peptide at pIII-terminus was selected for mediated synthesis of zero valent (ZV) Fe nanoparticles (NPs) with the wild M13 as control. Under a reducing environment, uniformly dispersed ZVFeNPs with diameter of 5–10 nm were both synthesized and the morphologies after annealing were confirmed to be face-centered cubic type. The synthesized FeNPs mediated by the two phages showed no significant difference, revealing that the pVIII capsid did dominant contribution to metal binding in comparison with the pIII. A novel pVIII-engineered M13 containing AAEEEDPAK at terminus, named as 4ED-pVIII-M13, was constructed and it carried one more negatively charged residue than the wild one (AEGDDPAK). Metal adsorption quantification showed that the binding affinity of the 4ED-pVIII-M13 toward Ag and Ni ions improved to 62 and 18 % from original 21 and 6 %, respectively. The binding affinity toward Fe remained constant (∼85 %). ZVFe–Ag bi-NPs were successfully synthesized through mediation of 4ED-pVIII-M13. Particularly, the Fe:Ag ratio in the bi-NPs was conveniently controlled through changing the molar concentration of FeCl{sub 2} and AgNO{sub 3} solution before reduction.

  12. Direct pollution cost assessment of cruising tourism in the Croatian Adriatic

    OpenAIRE

    Hrvoje Carić

    2010-01-01

    Cruise tourism is one of the fastest growing sectors of the tourism industry and one that has significant environmental, economic and social impacts on target destinations. Yet, tourism decision makers, developers and managers rarely incorporate or estimate environmental impacts in their tourism development planning. Indeed, the analysis of the resulting resource exploitation is rarely undertaken until carrying capacity is breached and attractiveness diminished. In this article an assessment ...

  13. Nearfield Unsteady Pressures at Cruise Mach Numbers for a Model Scale Counter-Rotation Open Rotor

    Science.gov (United States)

    Stephens, David B.

    2012-01-01

    An open rotor experiment was conducted at cruise Mach numbers and the unsteady pressure in the nearfield was measured. The system included extensive performance measurements, which can help provide insight into the noise generating mechanisms in the absence of flow measurements. A set of data acquired at a constant blade pitch angle but various rotor speeds was examined. The tone levels generated by the front and rear rotor were found to be nearly equal when the thrust was evenly balanced between rotors.

  14. Measles outbreak on a cruise ship in the western Mediterranean, February 2014, preliminary report.

    Science.gov (United States)

    Lanini, S; Capobianchi, M R; Puro, V; Filia, A; Del Manso, M; Karki, T; Nicoletti, L; Magurano, F; Derrough, T; Severi, E; Bonfigli, S; Lauria, Fn; Ippolito, G; Vellucci, L; Pompa, M G

    2014-03-13

    A measles outbreak occurred in February 2014 on a ship cruising the western Mediterranean Sea. Overall 27 cases were reported: 21 crew members, four passengers.For two cases the status crew or passenger was unknown. Genotype B3 was identified. Because of different nationalities of cases and persons on board,the event qualified as a cross-border health threat. The Italian Ministry of Health coordinated rapid response.Alerts were posted through the Early Warning and Response System.

  15. Cluster of invasive Neisseria meningitidis infections on a cruise ship, Italy, October 2012.

    Science.gov (United States)

    Stefanelli, P; Fazio, C; Neri, A; Isola, P; Sani, S; Marelli, P; Martinelli, C; Mastrantonio, P; Pompa, M G

    2012-12-13

    We describe a cluster of four cases of invasive meningococcal disease that occurred on a cruise ship sailing along the Italian coast in October 2012. All four cases were hospitalised with severe illness and one of them died. This report illustrates the importance of rapid implementation of emergency control measures such as administration of prophylaxis to all crew members and passengers to prevent the spread of the disease in such a close environment.

  16. Recombinant factor VIII Fc (rFVIIIFc) fusion protein reduces immunogenicity and induces tolerance in hemophilia A mice.

    Science.gov (United States)

    Krishnamoorthy, Sriram; Liu, Tongyao; Drager, Douglas; Patarroyo-White, Susannah; Chhabra, Ekta Seth; Peters, Robert; Josephson, Neil; Lillicrap, David; Blumberg, Richard S; Pierce, Glenn F; Jiang, Haiyan

    2016-03-01

    Anti-factor VIII (FVIII) antibodies is a major complication of FVIII replacement therapy for hemophilia A. We investigated the immune response to recombinant human factor VIII Fc (rFVIIIFc) in comparison to BDD-rFVIII and full-length rFVIII (FL-rFVIII) in hemophilia A mice. Repeated administration of therapeutically relevant doses of rFVIIIFc in these mice resulted in significantly lower antibody responses to rFVIII compared to BDD-rFVIII and FL-rFVIII and reduced antibody production upon subsequent challenge with high doses of rFVIIIFc. The induction of a tolerogenic response by rFVIIIFc was associated with higher percentage of regulatory T-cells, a lower percentage of pro-inflammatory splenic T-cells, and up-regulation of tolerogenic cytokines and markers. Disruption of Fc interactions with either FcRn or Fcγ receptors diminished tolerance induction, suggesting the involvement of these pathways. These results indicate that rFVIIIFc reduces immunogenicity and imparts tolerance to rFVIII demonstrating that recombinant therapeutic proteins may be modified to influence immunogenicity and facilitate tolerance.

  17. Clinicopathological characterization of experimental infection in chickens with sub-genotype VIIi Newcastle disease virus isolated from peafowl.

    Science.gov (United States)

    Desingu, P A; Singh, S D; Dhama, K; Kumar, O R Vinodh; Malik, Y S; Singh, R

    2017-04-01

    Newcastle disease (ND) is an economically important viral disease distressing poultry industry across the globe. Herein, we report the clinicopathology of sub-genotype VIIi Newcastle disease virus (NDV) isolated from peafowl in chickens. The virus isolate produced systemic infection with prominent tropism in visceral organs in chicken, confirmed on the basis of gross and microscopic lesions, and immunohistochemistry findings. The experimentally infected chickens exhibited 100% mortality with severe hemorrhagic lesions in the proventriculus and intestine, especially marked lymphocytolysis in spleen and bursa. The virus could be re-isolated from the cloacal swabs of infected chickens during 4th to 6th dpi (on 6th dpi all birds died), and all were tested positive in conventional RT-PCR. This is the first report on clinicopathology of NDV isolated from peafowl and/or sub-genotype VIIi NDV in experimentally infected chickens. Explorative epidemiological and molecular studies are suggested to screen wild peafowls and poultry flocks of the country for establishing the occurrence of this sub-genotype and opting for appropriate prevention and control strategies. Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. Changes of androgen receptor expression in stages VII-VIII seminiferous tubules of rat testis after exposure to methamphetamine

    Directory of Open Access Journals (Sweden)

    Sutita Nudmamud-Thanoi

    2016-06-01

    Full Text Available Methamphetamine (METH is toxic not only to the central nervous system but also to the reproductive system. This study aimed to investigate the effect of METH administration on androgen receptor (AR expression in the seminiferous tubules (stages VII-VIII and Leydig cells. 24 Sprague-Dawley rats were equally divided into 4 groups; control, acute doseMETH binge (AB-METH, escalating dose-METH (ED-METH and escalating dose-METH binge (ED-METH binge groups. The expressions of AR were examined using immunohistochemical staining. AR expressions in round and elongated spermatids showed significant decreases in ED-METH binge and AB-METH groups. In Leydig’s cells, AR expressions were significantly decreased in both ED-METH and ED-METH binge groups. These results could reflect the effect of METH on the expressions of AR in both round and elongated spermatids of stages VII-VIII seminiferous tubule and Leydig’s cells. Decreases of AR expressions in those cells of METH-treated animals may interrupt spermatogenesis at these stages.

  19. Il manoscritto Chigiano L VIII 305 della Biblioteca Apostolica Vaticana e la «Vita nuova»

    Directory of Open Access Journals (Sweden)

    Donato Pirovano

    2015-07-01

    Full Text Available L’articolo nasce a margine della nuova edizione commentata, con testo criticamente rivisto, della Vita nuova di Dante nell’àmbito della «Nuova Edizione Commentata delle Opere di Dante» (vd. Roma, Salerno Editrice, 2015. Viene spiegato in che misura il codice Chigiano L VIII 305 della Biblioteca Apostolica Vaticana (siglato K è stato utilizzato come manoscritto di riferimento per le varianti di forma. A partire dal caso specifico viene poi proposta una riflessione sulla restituzione formale degli antichi testi a tradizione plurima e una discussione sui criteri editoriali, tra conservazione e leggibilità. The article comes on the sidelines of the new annotated edition with critically reviewed text of the Vita Nuova by Dante, in the scope of the «Nuova Edizione Commentata delle Opere di Dante» (see Roma, Salerno Editrice, 2015. The author explains how the code Chigiano L VIII 305 of the Biblioteca Apostolica Vaticana (signed K was used as a reference manuscript for the formal variants. From the specific example the author proposes a reflection on the formal restituition of the plural tradition ancient texts and a discussion on the editorial criteria, between conservation and readability. 

  20. Pisanello’s hat. The costume and weapons depicted in Pisanello’s medal for John VIII Palaeologus. A discussion of the saber and related weapons

    Directory of Open Access Journals (Sweden)

    Alexander, David

    2004-12-01

    Full Text Available A group of six drawings by the Veronese artist Antonio Pisano depicted the Bizantine Emperor John VIII Paleologus wearing costumes and weapons according to islamic types. This paper studies these objects in relation to other contemporary islamic weapons.

    Un grupo de seis dibujos del artista veronés Antonio Pisano representa al emperador bizantino Juan VIII Paleólogo vestido y armado según modelos islámicos. Este trabajo estudia dichos objetos en relación a otras armas islámicas contemporáneas.

  1. A Two Element Laminar Flow Airfoil Optimized for Cruise. M.S. Thesis

    Science.gov (United States)

    Steen, Gregory Glen

    1994-01-01

    Numerical and experimental results are presented for a new two-element, fixed-geometry natural laminar flow airfoil optimized for cruise Reynolds numbers on the order of three million. The airfoil design consists of a primary element and an independent secondary element with a primary to secondary chord ratio of three to one. The airfoil was designed to improve the cruise lift-to-drag ratio while maintaining an appropriate landing capability when compared to conventional airfoils. The airfoil was numerically developed utilizing the NASA Langley Multi-Component Airfoil Analysis computer code running on a personal computer. Numerical results show a nearly 11.75 percent decrease in overall wing drag with no increase in stall speed at sailplane cruise conditions when compared to a wing based on an efficient single element airfoil. Section surface pressure, wake survey, transition location, and flow visualization results were obtained in the Texas A&M University Low Speed Wind Tunnel. Comparisons between the numerical and experimental data, the effects of the relative position and angle of the two elements, and Reynolds number variations from 8 x 10(exp 5) to 3 x 10(exp 6) for the optimum geometry case are presented.

  2. Hydrography in the Mediterranean Sea during a cruise with RV POSEIDON in April 2014

    Directory of Open Access Journals (Sweden)

    D. Hainbucher

    2015-05-01

    Full Text Available We report on data from an oceanographic cruise in the Mediterranean Sea on the German research vessel POSEIDON in April 2014. Data were taken on a west–east section starting at the Strait of Gibraltar and ending south-east of Crete as well on sections in the Ionian and Adriatic Sea. The objectives of the cruise were twofold; long-term variations of the Levantine Intermediate Water (LIW and the deep water masses of the Eastern Mediterranean Sea were investigated. The measurements include salinity, temperature, oxygen and currents and were conducted with a CTD/rosette system, an underway CTD and an ADCP. The sections are on tracks which have been sampled during several other cruises, thus supporting the opportunity to investigate the long term temporal development of the different variables. The use of an underway CTD made it possible to conduct measurements of temperature and salinity with a high resolution of 6 nm and a vertical resolution of 1 dbar for the upper 800 m of the water column.

  3. 15 Years of Oceanography in the Azores: From Oceanographic Cruises to an Integrated Approach

    Science.gov (United States)

    Juliano, M. F.; Alves, M.; Simões, A.; Rodeia, J.

    2006-07-01

    Oceanographic research in the Azores has only started 15 years ago. Nevertheless, it has been possible to evolve from the typical "research cruise approach" to a more versatile and integrated approach, that has proofed to be further rewarding and adequate for the size and resources of the group. The Azorean Oceanographers have participated and conducted several research cruises since 1990, and anticipating the importance of correlating in situ data with satellite data has lead us to plan the last of these, so that there was a coincidence of transects, moorings and satellite ground tracks. On the other hand, the group has invested in data processing and merging hydrological historical data with the results from the above mentioned oceanographic cruises, creating a hydrological database for the whole Atlantic Ocean. Also, through the application of a novel methodology and approach, the group has contributed to a new vision of the currents systems in the South Atlantic Ocean, namely with the newly found St. Helena Current, the congener of the Azores Current. As a result of this continued shift in methodology, the group is now using an integrated approach where field data, model output and processed altimeter data are merged to produce operational oceanography products, such as sea state charts and daily oceanic current charts.

  4. Acute gastrointestinal haemorrhage on board a cruise ship in the Antarctic Peninsula.

    Science.gov (United States)

    Carron, Mathieu; Globokar, Peter; Sicard, Bruno A

    2016-01-01

    Antarctic tourism on board cruise ships has expanded since the 1990s, essentially in the Antarctic Peninsula. Due to remoteness, medical cases may evolve into life threatening conditions as emergency medical evacuations are challenging. We discuss the case of a young crew member who suddenly fainted with an epigastric pain and abundant rectal bleeding while on board a cruise ship heading to the Deception Island (62°57.6 South, 60°29.5 West), 44 h away from Ushuaia by sea. A medical evacuation was necessary to save the patient whose haemoglobin level rapidly decreased from 11 g/dL to 8.7 g/dL over an 8 h period due to uncontrolled gastrointestinal bleeding. Following discussions between the French, Chilean and Argentinean Medical Top Side Support and Maritime Rescue Authorities and despite poor weather conditions, an emergency medical evacuation by air to Chile was made possible. The evacuation, which was 2 days shorter compared to an evacuation by sea, allowed the patient to reach a hospital facility in time to save his life whereas he decompensated in haemorrhagic shock. As passengers on cruise ships are typically elderly and often following anticoagulant therapies, the risk of bleeding is most important. Facing a gastric haemorrhage, a transfusion is often required. In remote areas, transfusion of fresh whole blood to stabilize a critical patient until he reaches a hospital must be considered.

  5. Shared Semantics for Oceanographic Research: Development of Standard ``Cruise-Level'' Metadata

    Science.gov (United States)

    Arko, R. A.; Milan, A.; Chandler, C. L.; Miller, S. P.; Ferrini, V.; Mesick, S.; Mize, J.; Paver, C.; Sullivan, B.; Sweeney, A.

    2010-12-01

    There is a general need in the ocean science community for a widely accepted standards-based “cruise-level” metadata profile that describes the basic elements of a seagoing expedition (e.g. cruise identifier, vessel name, operating institution, dates/ports, navigation track, survey targets, science party, funding sources, scientific instruments, daughter platforms, and data sets). The need for such a profile is increasingly urgent as seagoing programs become more complex and interdisciplinary; funding agencies mandate public dissemination of the resulting data; and data centers link post-field/derived products to original field data sets. We are developing a standard implementation for cruise-level metadata that serves the needs of multiple U.S. programs, in an effort to promote interoperability and facilitate collaboration. Testbed development has focused on the Rolling Deck to Repository (R2R) and Extended Continental Shelf (ECS) programs - both tasked with routinely documenting and archiving large volumes of data from a wide array of U.S. research vessels - and draws from the cruise-level metadata profile published by the University-National Oceanographic Laboratory System (UNOLS) Data Management Best Practices Committee in 2008. Our XML implementation is based on the ISO 19115-2:2009 standard for geospatial metadata, with controlled vocabulary terms directly embedded as Uniform Resource Identifier (URI) references that can be validated in e.g. ISO Schematron. Our choice of the ISO standard reflects ANSI's adoption of the ISO 19115 North American Profile in 2009, and the adoption of ISO 19115 by related programs including the Integrated Ocean Drilling Program (IODP) and the SeaDataNet program in Europe. We envision a hierarchical framework where a single “cruise-level” record is linked to multiple “dataset-level” records that may be published independently. Our results published online will include a best practices guide for authoring records

  6. Patient preference for needleless factor VIII reconstitution device: the Italian experience

    Directory of Open Access Journals (Sweden)

    Roberto Musso

    2010-07-01

    Full Text Available Roberto Musso1, Rita Santoro2, Antonio Coppola3, Maura Marcucci4, Gianluca Sottilotta5, Roberto Targhetta6, Ezio Zanon7, Massimo Franchini81Centro Emofilia, Istituto di Ematologia Università degli Studi di Catania, Ospedale Ferrarotto, Catania, Italy; 2Haemophilia Centre, Hemostasis and Thrombosis Service, Department of Haematology and Oncology, Azienda Ospedaliera “Pugliese-Ciaccio”, Catanzaro, Italy; 3Regional Reference Centre for Coagulation Disorders, Federico II University, Naples, Italy; 4Internal and Vascular Medicine, University of Perugia, Perugia, Italy; 5Hemophilia Centre, Hemostasis and Thrombosis Service, Azienda Ospedaliera “Bianchi-Melacrino-Morelli”, Reggio Calabria, Italy; 6Centro Emofilia Struttura Semplice Patologie della Coagulazione, Ospedale Microcitemico, Cagliari, Italy; 7Centro Emofilia, Clinica Medica II, Azienda Ospedaliera di Padova, Padova, Italy; 8Immunohaematology and Transfusion Centre, Department of Pathology and Laboratory Medicine, University Hospital of Parma, Parma, ItalyBackground: Needlestick injuries, mostly due to unsafe needle devices, are a frequent adverse event among health care workers and patients on chronic treatment, such as hemophiliacs. To improve the safety of these procedures, a needleless reconstitution system, Bio-Set® has been implemented for the sucrose-formulated recombinant factor VIII (rFVIII-FS Kogenate® Bayer (Bayer Healthcare, Berlin, Germany. The aim of this study was to collect patients’ satisfaction and safety data regarding the administration of rFVIII-FS with this new device.Methods: This was a multicenter, prospective, postmarketing surveillance study collecting data from seven Italian Haemophilia Centers within the framework of an international project involving patients from nine European countries. The patients were asked to fill out two preference questionnaires (one assessing the old method and one assessing the new method directly after the training and two

  7. Angioma cavernoso do meato acústico interno envolvendo o complexo VII e VIII nervos cranianos: relato de caso Cavernous hemangioma of the internal auditory canal encasing the VII and VIII cranial nerve complex: case report

    Directory of Open Access Journals (Sweden)

    Roberto Leal Silveira

    2005-03-01

    Full Text Available Relata-se o caso de homem de 21 anos, leucoderma, portador de angioma cavernoso do meato acústico interno direito envolvendo o complexo VII e VIII nervos cranianos tratado cirurgicamente. Apenas 18 casos de angiomas cavernosos dessa localização foram relatados na literatura. São comentados os aspectos clínicos, o diagnóstico diferencial e o tratamento.We report the surgically treated case of a 21-year-old caucasian male harboring a cavernous hemangioma of the right internal auditory canal encasing the seventh and eighth cranial nerves complex. Only 18 cases of cavernous hemangiomas of this location have been previously reported. The clinical features, the differential diagnosis and the treatment are discussed.

  8. Recurrent myocardial infarctions in a young football player secondary to thrombophilia, associated with elevated factor VIII activity

    Directory of Open Access Journals (Sweden)

    Vacek TP

    2014-10-01

    Full Text Available Thomas P Vacek, Shipeng Yu, Shahnaz Rehman, Blair P Grubb, Daniel Kosinski, Cherian Verghese, Ehab A Eltahawy, Shafiq Qaiser Department of Medicine, University of Toledo Medical Center, Toledo, OH, USAAbstract: Myocardial infarction (MI due to coronary atherosclerosis in young adults is uncommon; rare causes such as cocaine abuse, arterial dissection, and thromboembolism should be considered. A 21-year-old football player, and otherwise healthy African American man, developed chest pain during exercise while bench-pressing 400 lbs. Acute MI was diagnosed based on physical examination, electrocardiography findings, and elevated cardiac enzymes. Coronary arteriography showed a thrombus occluding the proximal left anterior descending artery (LAD. Aggressive antiplatelet therapy with aspirin, clopidogrel, and eptifibatide was pursued, in addition to standard post-MI care. This led to the successful resolution of symptoms and dissolution of the thrombus, demonstrated by repeat coronary arteriography. Five months later, he presented with similar symptoms during exercise after lifting heavy weights, and was found to have another acute MI. Coronary arteriography again showed a thrombus occluding the LAD. No evidence of coronary artery dissection or vasospasm was found. Only mild atherosclerotic plaque burden was observed on both occasions by intravascular ultrasound. A bare metal stent was placed at the site as it was thought this site had acted as a nidus for small plaque rupture and thrombus formation. Elevated serum factor VIII activity at 205% (reference range 60%–140% was found, a rare cause of hypercoagulability. Further workup revealed a patent foramen ovale during a Valsalva maneuver by transesophageal echocardiography. Both events occurred during weight lifting, which can transiently increase right heart pressure in a similar way to the Valsalva maneuver. In light of all the findings, we concluded that an exercise-related increase in factor

  9. Retsensioon : Riigikogu VII, VIII ja IX koosseis. Statistikat ja kommentaare. Peatoimetaja Aaro Mõttus. Tallinn : Riigikogu Kantselei, Eesti Rahvusraamatukogu, 2004. 326 lk. / Mart Nutt

    Index Scriptorium Estoniae

    Nutt, Mart, 1962-

    2004-01-01

    Arvustus: Riigikogu VII, VIII ja IX koosseis. Statistikat ja kommentaare / peatoim. Aaro Mõttus. Tallinn : Riigikogu Kantselei, Eesti Rahvusraamatukogu, 2004. - Lisatud: Ott Heinapuu tutvustav sõnum retsenseeritud väljaande kohta lk. 193. - Vt. ka: http://www.riigikogu.ee/rito/index.php?id=11357

  10. Impact of quality control matrix effect: application to the calculation of uncertainty of measurement in one-stage clotting factor VIII assay.

    Science.gov (United States)

    Sobas, Frédéric; Benattar, Norbert; Bellisario, Audrey; Marin, Sylvie; Nougier, Christophe; Lienhart, Anne; Négrier, Claude

    2010-07-01

    In Europe, the ISO 15 189 standard requires uncertainty of measurement to be calculated for all measurands. We calculated the analytical imprecision and bias of our factor VIII coagulometric assay method between 5 and 80 U/dl, using plasmas expected to be at 5, 30 and 80 U/dl of factor VIII. We implemented Meijer et al.'s [Clin Chem 2002; 48:1011-1015] long-term coefficient of variance, bias and also uncertainty of measurement calculations. Assessments used reference plasma diluted in severe haemophilic plasma, in immunodepleted factor VIII-deficient plasma and in bovine serum albumin. With plasmas diluted in severe haemophilic and immunodepleted factor VIII-deficient plasma, calculated uncertainty of measurement was 10% compared with 15% (i.e., 50% greater) for plasma diluted in albumin buffer or as calculated from European Concerted Action on Thrombosis consensus values. It is thus important to approximate the patient sample matrix to obtain as precise an estimation as possible of assay method uncertainty of measurement.

  11. Report on corrections and future considerations for Appendices II–VIII of the International Code of Nomenclature for Algae, Fungi, and Plants

    Science.gov (United States)

    For the first time, the main text and Appendices II–VIII of the International Code of Nomenclature were separately published following decisions of the Melbourne Nomenclature Section, which contributed to subsequent development of an online resource capable of producing the Appendices in real time. ...

  12. PENGARUH MODEL PEMBELAJARAN INQUIRY TRAINING TERHADAP HASIL BELAJAR SISWA PADA MATERI POKOK USAHA DAN ENERGI KELAS VIII MTS N-3 MEDAN

    Directory of Open Access Journals (Sweden)

    Ratni Sirait

    2012-06-01

    36 orang. Hasil analisis data melalui uji t menunjukkan bahwa diperoleh thitung = 1,71 dan ttabel = 1,67, sehingga thitung > ttabel, artinya Ha diterima. Dapat disimpulkan ada pengaruh terhadap hasil belajar siswa dengan menggunakan model pembelajaran inquiry training pada materi pokok usaha dan energi kelas VIII semester I MTs N 3 Medan.

  13. The Regularities of Phase and Structural Transformation in Binary Titanium Alloys with Metals of IV—VIII Groups of the Periodic Table

    National Research Council Canada - National Science Library

    Dobromyslov, A V

    2008-01-01

    Martensitic β→α′(α″) and β→ω transformation in a series of Ti-base alloys with transition metals of groups IV-VIII have been investigated using the techniques of X-ray diffraction, optical and transmission electron microscopy...

  14. Mechanistic aspects of Os(VIII) catalysed oxidation of loop diuretic drug furosemide by Ag(III) periodate complex in aqueous alkaline medium

    Indian Academy of Sciences (India)

    Shweta J Malode; Nagaraj P Shetti; Sharanappa T Nandibewoor

    2012-03-01

    The kinetics of oxidation of a loop diuretic drug furosemide (Fur) by diperiodatoargentate(III) (DPA) has been investigated in the presence of osmium(VIII) (Os(VIII)) used as homogeneous catalyst in alkaline medium at a constant ionic strength of 0.20mol dm-3 spectrophotometrically attached with HI-TECH SFA-12 stopped flow accessory. The stoichiometry was 1:2 (Fur:DPA). The order of the reaction with respect to [DPA] was unity while the order with respect to [Fur] was less than unity over the concentration range studied. The rate increased with an increase in [OH−] and decreased with an increase in [IO$^{−}_{4}$]. The order with respect to [Os(VIII)] was unity. The oxidation products were identified as 2-(4-carboxy-2-oxo-but-3-enylamino)-4-chloro-5-sulfamoyl-benzoic acid and Ag(I). A suitable mechanism was proposed. The reaction constants involved in the different steps of the reaction mechanism were calculated. Kinetic experiments suggest that [Ag(H2IO6)(H2O)2] is the reactive silver(III) species and [OsO4(OH)2]2− is the reactive Os(VIII) species.

  15. EFFICACY AND SAFETY OF A MONOCLONAL PURIFIED FACTOR-VIII CONCENTRATE - 5-YEAR FOLLOW-UP IN PREVIOUSLY TREATED HIV-NEGATIVE HEMOPHILIACS

    NARCIS (Netherlands)

    SMID, WM; VANDERMEER, J; HALIE, MR

    1995-01-01

    The efficacy and safety of a monoclonal purified factor VIII concentrate (Hemofil M) were assessed in a historically controlled study in 22 HIV-negative patients with haemophilia A, previously treated with various concentrates. Data from 5 years of follow-up were compared with those from the precedi

  16. Efficacy and safety of a monoclonal purified factor-VIII concentrate - 5-year follow-up in previously treated HIV-negative hemophiliacs

    NARCIS (Netherlands)

    Smid, W. M.; van der Meer, J.; Halie, M. R.

    1995-01-01

    The efficacy and safety of a monoclonal purified factor VIII concentrate (Hemofil M) were assessed in a historically controlled study in 22 HIV-negative patients with haemophilia A, previously treated with various concentrates. Data from 5 years of follow-up were compared with those from the

  17. Is Octavalent Pu(VIII) Possible? Mapping the Plutonium Oxyfluoride Series PuO(n)F(8-2n) (n = 0-4).

    Science.gov (United States)

    Huang, Wei; Pyykkö, Pekka; Li, Jun

    2015-09-01

    While the oxidation state Pu(VIII) is shown to be less stable than Pu(V) in the PuO4 molecule, it is not clear if the more electronegative fluorine can help to stabilize Pu(VIII). Our calculations on PuO(n)F(8-2n) (n = 0-4) molecules notably confirm that PuO2F4 has both (1)D(4h) and (5)C(2v) minima with the oxidation states Pu(VIII) and Pu(V), respectively, with the latter having lower energy. The hybrid-DFT, CCSD(T), and CASSCF methods all give the same result. The results conform to a superoxide ligand when n ≥ 2. PuF8 in a (1)O(h) state can decompose to PuF6 and F2, and PuOF6 in a (1)C(2v) state also can break down to PuF6 and 1/2 O2. The Pu(VIII) anion PuO2F5(-) does have a D(5h) minimum, which also lies above a (5)C(2v) Pu(V) peroxide structure. However, the energy differences between the different minima are not large, indicating that metastable species with oxidation states higher than Pu(V) cannot be completely excluded.

  18. Retsensioon : Riigikogu VII, VIII ja IX koosseis. Statistikat ja kommentaare. Peatoimetaja Aaro Mõttus. Tallinn : Riigikogu Kantselei, Eesti Rahvusraamatukogu, 2004. 326 lk. / Mart Nutt

    Index Scriptorium Estoniae

    Nutt, Mart, 1962-

    2004-01-01

    Arvustus: Riigikogu VII, VIII ja IX koosseis. Statistikat ja kommentaare / peatoim. Aaro Mõttus. Tallinn : Riigikogu Kantselei, Eesti Rahvusraamatukogu, 2004. - Lisatud: Ott Heinapuu tutvustav sõnum retsenseeritud väljaande kohta lk. 193. - Vt. ka: http://www.riigikogu.ee/rito/index.php?id=11357

  19. Theoretical studies of the atomic transitions in boron-like ions: Mg VIII, Si X and S XII

    Energy Technology Data Exchange (ETDEWEB)

    Nataraj, H S [Non-Accelerator Particle Physics Group, Indian Institute of Astrophysics, Bangalore-34 (India); Sahoo, B K [Max Planck Institute for the Physics of Complex Systems, Noethnitzer Street 38, D-01187 Dresden (Germany); Das, B P [Non-Accelerator Particle Physics Group, Indian Institute of Astrophysics, Bangalore-34 (India); Chaudhuri, R K [Non-Accelerator Particle Physics Group, Indian Institute of Astrophysics, Bangalore-34 (India); Mukherjee, D [Department of Physical Chemistry, Indian Association for Cultivation of Science, Kolkata-700 032 (India)

    2007-08-14

    In this paper, we have carried out the calculations of the weighted oscillator strengths and the transition probabilities for a few low-lying transitions of boron-like ions: Mg VIII, Si X and S XII, which are astrophysically important, particularly in the atmosphere of the solar corona. We have employed an all-order relativistic many-body theory called the relativistic coupled-cluster theory to calculate very precisely these atomic quantities of astrophysical interest. We have reported for the first time the transition probabilities for some forbidden transitions which are unavailable in the literature, either theoretically or experimentally. We also discuss the physical effects associated with these transitions. Our data can be used for the identification of spectral lines arising from the coronal atmospheres of the Sun and Sun-like stars having an extended corona.

  20. Tranexamic acid combined with recombinant factor VIII increases clot resistance to accelerated fibrinolysis in severe hemophilia A

    DEFF Research Database (Denmark)

    Hvas, Anne-Mette; Sørensen, Hanne Thykjær; Norengaard, Lisbeth

    2007-01-01

    BACKGROUND: Most patients with severe hemophilia A suffer from a profoundly compromised hemostatic response. In addition to both the delayed and slow development of a clot, previous studies have documented that severe hemophilia A is also associated with reduced clot stability. OBJECTIVES: We...... examined whether the clot stability in hemophiliacs could be improved by treatment with tranexamic acid (TXA) in combination with recombinant factor VIII (rFVIII). PATIENTS/METHODS: Baseline blood samples were obtained from eight males with severe hemophilia A. Thereafter, a bolus injection of r...... the elasticity curve increased 5-fold after rFVIII and 24-fold after addition of TXA. CONCLUSIONS: The study demonstrates that simultaneous treatment with TXA and rFVIII significantly improves the clot stability in patients with hemophilia A. Udgivelsesdato: December...

  1. The Effect Of Core Polarization On The Population Of The Rydberg States Of ArVIII Ions Escaping Solid Surfaces

    Science.gov (United States)

    Galijas, S. M. D.; Nedeljkovic, N. N.; Majkic, M. D.; Bunjac, A. B.

    2010-07-01

    The appearance of maxima at nA = nmax in the population distributions for the Rydberg states of multiply charged ions ArVIII escaping solid surfaces at intermediate velocities ( v ˜1 a.u.) is discussed. Within the framework of the time-symmetrized two-state vector model, in which the state of a single active electron is described by two wave functions 1 ? and 2 ? , the regular maxima appear as a consequence of the electron tunneling through the potential barrier created between the ionic core and the polarized solid. The pronounced peaks (resonances) in the population distributions are addressed to the electron tunneling in the vicinity of the potential barrier top. The appropriate etalon equation method is used in the calculation of the function 1 ? ; the effect of core polarization is expressed via the function ?2.

  2. Recurrent myocardial infarctions in a young football player secondary to thrombophilia, associated with elevated factor VIII activity.

    Science.gov (United States)

    Vacek, Thomas P; Yu, Shipeng; Rehman, Shahnaz; Grubb, Blair P; Kosinski, Daniel; Verghese, Cherian; Eltahawy, Ehab A; Shafiq, Qaiser

    2014-01-01

    Myocardial infarction (MI) due to coronary atherosclerosis in young adults is uncommon; rare causes such as cocaine abuse, arterial dissection, and thromboembolism should be considered. A 21-year-old football player, and otherwise healthy African American man, developed chest pain during exercise while bench-pressing 400 lbs. Acute MI was diagnosed based on physical examination, electrocardiography findings, and elevated cardiac enzymes. Coronary arteriography showed a thrombus occluding the proximal left anterior descending artery (LAD). Aggressive antiplatelet therapy with aspirin, clopidogrel, and eptifibatide was pursued, in addition to standard post-MI care. This led to the successful resolution of symptoms and dissolution of the thrombus, demonstrated by repeat coronary arteriography. Five months later, he presented with similar symptoms during exercise after lifting heavy weights, and was found to have another acute MI. Coronary arteriography again showed a thrombus occluding the LAD. No evidence of coronary artery dissection or vasospasm was found. Only mild atherosclerotic plaque burden was observed on both occasions by intravascular ultrasound. A bare metal stent was placed at the site as it was thought this site had acted as a nidus for small plaque rupture and thrombus formation. Elevated serum factor VIII activity at 205% (reference range 60%-140%) was found, a rare cause of hypercoagulability. Further workup revealed a patent foramen ovale during a Valsalva maneuver by transesophageal echocardiography. Both events occurred during weight lifting, which can transiently increase right heart pressure in a similar way to the Valsalva maneuver. In light of all the findings, we concluded that an exercise-related increase in factor VIII activity led to coronary arterial thrombosis in the presence of a small ruptured plaque. Alternatively, venous clots may have traversed the patent foramen ovale and occluded the LAD. In addition to continuing aggressive risk

  3. [Detection of alloantibodies against Factor VIII in plasma of patients with hemophilia A and its relationship with Factor VIIIC domain].

    Science.gov (United States)

    Zhang, Lu-Lu; Yu, Zi-Qiang; Wan, Chu-Cheng; Zhang, Wei; Zhang, Zheng-Hua; Ruan, Chang-Geng

    2013-10-01

    This study was purposed to detect the alloantibodies against Factor VIII (FVIII) by ELISA for estimating the incidence of the alloantibodies against Factor VIII (FVIII) in patients with hemophilia A, and to investigate the relationship between factor VIIIC domain and alloantibodies. Total of 140 patients with hemophilia A and 80 normal controls were enrolled in this study, among them plasma FVIII level of 84 patients was less than 1%, plasma FVIII level of 34 patients was between 1% and 5%, and plasma FVIII level of 22 patients was more than 5%. All patients were treated with plasma-derived FVIII concentrate or plasma before. The ELISA plate was coated with McAb (SZ-132) against FVIII prepared in our laboratory, then human recombinant FVIII concentrates were applied. After incubation in room temperature for 2 hours, diluted plasma samples and HRP-conjugated goat anti-human IgG were added successively, finally A490 was recorded. The threshold of alloantibody of patient plasma was set as mean value>3 SD more than control. The plate was coated with antibody against His, then human recombinant FVIII-C1C2 prepared in our laboratory was added. After incubation in room temperature for 2 hours, diluted plasma samples and HRP-conjugated goat anti-human IgG were added successively, finally A490 were recorded. The threshold was set as the mean value>3 SD more than control. The results showed that the alloantibodies against FVIII were found in 56 patients (40%) by ELISA, and 82.1% (46/56) of this kind of alloantibody could interact with the C domain of FVIII. It is concluded that C domain of FVIII is one of the primary binding sites for the alloantibodies against FVIII in Chinese patients with hemophilia A.

  4. Targeting factor VIII expression to platelets for hemophilia A gene therapy does not induce an apparent thrombotic risk in mice.

    Science.gov (United States)

    Baumgartner, C K; Mattson, J G; Weiler, H; Shi, Q; Montgomery, R R

    2017-01-01

    Essentials Platelet-Factor (F) VIII gene therapy is a promising treatment in hemophilia A. This study aims to evaluate if platelet-FVIII expression would increase the risk for thrombosis. Targeting FVIII expression to platelets does not induce or elevate thrombosis risk. Platelets expressing FVIII are neither hyper-activated nor hyper-responsive. Background Targeting factor (F) VIII expression to platelets is a promising gene therapy approach for hemophilia A, and is successful even in the presence of inhibitors. It is well known that platelets play important roles not only in hemostasis, but also in thrombosis and inflammation. Objective To evaluate whether platelet-FVIII expression might increase thrombotic risk and thereby compromise the safety of this approach. Methods In this study, platelet-FVIII-expressing transgenic mice were examined either in steady-state conditions or under prothrombotic conditions induced by inflammation or the FV Leiden mutation. Native whole blood thrombin generation assay, rotational thromboelastometry analysis and ferric chloride-induced vessel injury were used to evaluate the hemostatic properties. Various parameters associated with thrombosis risk, including D-dimer, thrombin-antithrombin complexes, fibrinogen, tissue fibrin deposition, platelet activation status and activatability, and platelet-leukocyte aggregates, were assessed. Results We generated a new line of transgenic mice that expressed 30-fold higher levels of platelet-expressed FVIII than are therapeutically required to restore hemostasis in hemophilic mice. Under both steady-state conditions and prothrombotic conditions induced by lipopolysaccharide-mediated inflammation or the FV Leiden mutation, supratherapeutic levels of platelet-expressed FVIII did not appear to be thrombogenic. Furthermore, FVIII-expressing platelets were neither hyperactivated nor hyperactivatable upon agonist activation. Conclusion We conclude that, in mice, more than 30-fold higher levels of

  5. PENGARUH FAKTOR INTERNAL DAN EKSTERNAL SISWA TERHADAP HASIL BELAJAR MATA PELAJARAN IPS KELAS VIII DI MTS N 02 SEMARANG

    Directory of Open Access Journals (Sweden)

    Erlina Indriasari

    2014-08-01

    Full Text Available Hasil belajar mempunyai fungsi sebagai indikator untuk mengetahui sejauh manakompetensi yang telah dikuasai oleh siswa. Tujuan dari penelitian ini adalah untukmengetahui, menganalisis dan mengkaji pengaruh motivasi, sikap, lingkungankeluarga dan lingkungan sekolah secara parsial dan secara simultan terhadap hasilbelajar mata pelajaran IPS. Penelitian ini menggunakan metode kuantitatif. Sampelpenelitian ini berjumlah 161 siswa kelas VIII. pengumpulan data melalui angketdan dokumentasi. Analisis data dengan menggunakan analisis Regresi LinierSederhana dan Berganda. Hasil penelitian menunjukkan (1 ada pengaruh yangsignifikan antara motivasi dan hasil belajar sebesar 8,47%, (2 ada pengaruh yangsignifikan antara sikap dan hasil belajar sebesar 18,57%, (3 ada pengaruh yang signifikanantara lingkungan keluarga dan hasil belajar sebesar 10,62%, (4 ada pengaruhyang signifikan antara lingkungan sekolah dan hasil belajar sebesar4,28%, dan(5 ada pengaruh yang signifikan antara motivasi, sikap, lingkungan keluarga, danlingkungan sekolah dan hasil belajar IPS kelas VIII di MTs N 02 Semarang secarabersama-sama sebesar 49,3%. Learning outcomes has the function as an indicator to determine the extent of competencethat has been mastered by the student. The purpose of this study is to investigate, analyzeand assess the influence of motivation, attitude, family environment and school environmentpartially and simultaneously to the learning outcomes of the eye social studies. This study usesquantitative methods. The study sample totaled 161 eighth grade students. collection of datathrough questionnaires and documentation. Analysis of the data using the Simple LinearRegression analysis and Regression. The results showed (1 no significant relationship betweenmotivation and learning outcomes of 8.47%, (2 no significant relationship between attitudesand learning outcomes of 18.57%, (3 no significant relationship between family environmentand learning outcomes of 10

  6. Alteration in lymphocyte responses, cytokine and chemokine profiles in chickens infected with genotype VII and VIII velogenic Newcastle disease virus.

    Science.gov (United States)

    Rasoli, Mehdi; Yeap, Swee Keong; Tan, Sheau Wei; Moeini, Hassan; Ideris, Aini; Bejo, Mohd Hair; Alitheen, Noorjahan Banu Mohamed; Kaiser, Pete; Omar, Abdul Rahman

    2014-01-01

    Newcastle disease (ND) is a highly contagious avian disease and one of the major causes of economic losses in the poultry industry. The emergence of virulent NDV genotypes and repeated outbreaks of NDV in vaccinated chickens have raised the need for fundamental studies on the virus-host interactions. In this study, the profiles of B and T lymphocytes and macrophages and differential expression of 26 immune-related genes in the spleen of specific-pathogen-free (SPF) chickens, infected with either the velogenic genotype VII NDV strain IBS002 or the genotype VIII NDV strain AF2240, were evaluated. A significant reduction in T lymphocyte population and an increase in the infiltration of IgM+ B cells and KUL01+ macrophages were detected in the infected spleens at 1, 3 and 4 days post-infection (dpi) (P<0.05). The gene expression profiles showed an up-regulation of CCLi3, CXCLi1, CXCLi2 (IL-8), IFN-γ, IL-12α, IL-18, IL-1β, IL-6, iNOS, TLR7, MHCI, IL-17F and TNFSF13B (P<0.05). However, these two genotypes showed different cytokine expression patterns and viral load. IBS002 showed higher viral load than AF2240 in spleen at 3 and 4dpi and caused a more rapid up-regulation of CXCLi2, IFN-γ, IL-12α, IL-18, IL-1β, iNOS and IL-10 at 3dpi. Meanwhile, the expression levels of CCLI3, CXCLi1, IFN-γ, IL-12α, IL-1β and iNOS genes were significantly higher in AF2240 at 4dpi. In addition, the expression levels of IL-10 were significantly higher in the IBS002-infected chickens at 3 and 4dpi. Hence, infection with velogenic genotype VII and VIII NDV induced different viral load and production of cytokines and chemokines associated with inflammatory reactions.

  7. Continuous infusion of porcine factor VIII in patients with haemophilia A and high-responding inhibitors: stability and clinical experience.

    Science.gov (United States)

    O'Gorman, P; Dimichele, D M; Kasper, C K; Mannucci, P M; Santagostini, E; Hay, C R

    2001-11-01

    A multicentre retrospective survey was conducted to assess the efficacy and side-effect profile of porcine factor VIII (pFVIII:C) given by continuous infusion (CI) to patients with congenital haemophilia A and inhibitors. Twenty-nine episodes in 18 patients were treated by CI of pFVIII:C. Efficacy was graded as good in 79% of infusions and fair in 17%. There was a failed response in only one episode. Fourteen percent of patients experienced transfusion reactions with bolus doses, but no reactions were observed in patients given CI. There were no severe reactions. All the reactions resolved following interruption of the infusion and administration of steroids. Premedication did not prevent reactions. In this series the median decrease in platelet count after bolus injection of pFVIII:C was -67 X 10(9) L(-1) compared with a median decrease of -2 x 109 L(-1) during the course of CI, thus, continuous infusion of pFVIII:C appears to have less effect on platelet count than bolus injection. An anamnestic response was associated with 77% of infusions. This high rate of anamnesis reflects patient selection, in that they were all known to have high-level high-responding FVIII inhibitors with cross-reactivity to pFVIII. After reconstitution, the pFVIII:C showed little loss in factor VIII activity in solution over a 24-h period. We conclude that pFVIII:C may be effectively administered by CI to patients with haemophilia A and high-responding FVIII inhibitors. CI is the probably the mode of administration of choice for intensive replacement therapy with pFVIII.

  8. Production of factor VIII by human liver sinusoidal endothelial cells transplanted in immunodeficient uPA mice.

    Directory of Open Access Journals (Sweden)

    Marina E Fomin

    Full Text Available Liver sinusoidal endothelial cells (LSECs form a semi-permeable barrier between parenchymal hepatocytes and the blood. LSECs participate in liver metabolism, clearance of pathological agents, immunological responses, architectural maintenance of the liver and synthesis of growth factors and cytokines. LSECs also play an important role in coagulation through the synthesis of Factor VIII (FVIII. Herein, we phenotypically define human LSECs isolated from fetal liver using flow cytometry and immunofluorescence microscopy. Isolated LSECs were cultured and shown to express endothelial markers and markers specific for the LSEC lineage. LSECs were also shown to engraft the liver when human fetal liver cells were transplanted into immunodeficient mice with liver specific expression of the urokinase-type plasminogen activator (uPA transgene (uPA-NOG mice. Engrafted cells expressed human Factor VIII at levels approaching those found in human plasma. We also demonstrate engraftment of adult LSECs, as well as hepatocytes, transplanted into uPA-NOG mice. We propose that overexpression of uPA provides beneficial conditions for LSEC engraftment due to elevated expression of the angiogenic cytokine, vascular endothelial growth factor. This work provides a detailed characterization of human midgestation LSECs, thereby providing the means for their purification and culture based on their expression of CD14 and CD32 as well as a lack of CD45 expression. The uPA-NOG mouse is shown to be a permissive host for human LSECs and adult hepatocytes, but not fetal hepatoblasts. Thus, these mice provide a useful model system to study these cell types in vivo. Demonstration of human FVIII production by transplanted LSECs encourages further pursuit of LSEC transplantation as a cellular therapy for the treatment of hemophilia A.

  9. The real-time complex cruise scene motion detection system based on DSP

    Science.gov (United States)

    Wu, Zhi-guo; Wang, Ming-jia

    2014-11-01

    Dynamic target recognition is an important issue in the field of image processing research. It is widely used in photoelectric detection, target tracking, video surveillance areas. Complex cruise scene of target detection, compared to the static background, since the target and background objects together and both are in motion, greatly increases the complexity of moving target detection and recognition. Based on the practical engineering applications, combining an embedded systems and real-time image detection technology, this paper proposes a real-time movement detection method on an embedded system based on the FPGA + DSP system architecture on an embedded system. The DSP digital image processing system takes high speed digital signal processor DSP TMS320C6416T as the main computing components. And we take large capacity FPGA as coprocessor. It is designed and developed a high-performance image processing card. The FPGA is responsible for the data receiving and dispatching, DSP is responsible for data processing. The FPGA collects image data and controls SDRAM according to the digital image sequence. The SDRAM realizes multiport image buffer. DSP reads real-time image through SDRAM and performs scene motion detection algorithm. Then we implement the data reception and data processing parallelization. This system designs and realizes complex cruise scene motion detection for engineering application. The image edge information has the anti-light change and the strong anti-interference ability. First of all, the adjacent frame and current frame image are processed by convolution operation, extract the edge images. Then we compute correlation strength and the value of movement offset. We can complete scene motion parameters estimation by the result, in order to achieve real-time accurate motion detection. We use images in resolution of 768 * 576 and 25Hz frame rate to do the real-time cruise experiment. The results show that the proposed system achieves real

  10. Rolling Deck to Repository (R2R): Building a Reference Cruise Catalog for the Research Fleet

    Science.gov (United States)

    Arko, R. A.; Clark, P. D.; Chandler, C. L.; Smith, S. R.

    2012-12-01

    The Rolling Deck to Repository (R2R) program is developing infrastructure to ensure the underway sensor data from NSF-supported oceanographic research vessels are routinely and consistently documented, preserved in long-term archives, and disseminated to the science community. A core element of this infrastructure is a master catalog of research vessels, cruises, sensor systems, underway datasets, navigation products, field reports, event logs, file formats, people, organizations, and funding awards that is maintained uniformly across the research fleet. All NSF-supported vessel operators now submit their underway cruise data and documentation directly to R2R, per the revised Division of Ocean Sciences (OCE) Sample and Data Policy published in 2011. R2R ingests this content into the fleet catalog, assigning globally unique and persistent identifiers at the cruise, dataset, and file (granule) levels. The catalog is aligned with community-standard vocabularies, working collaboratively with the NOAA Data Centers, UNOLS Office, and pan-European SeaDataNet project; and includes links to related data at other inter/national repositories. In response to community demand, we are extending the catalog to include instrument-specific metadata such as installation details, patch tests, and calibration results. The entire R2R catalog is published on the Web as "Linked Data", making it easily accessible to encourage integration with other repositories. Selected content is also published in formal metadata records according to ISO and W3C standards, suitable for submission to long-term archives. We are deploying both faceted (classification/filter) and Web map-based browse and search interfaces.

  11. Effect of emerging technology on a convertible, business/interceptor, supersonic-cruise jet

    Science.gov (United States)

    Beissner, F. L., Jr.; Lovell, W. A.; Robins, A. W.; Swanson, E. E.

    1986-01-01

    This study was initiated to assess the feasibility of an eight-passenger, supersonic-cruise long range business jet aircraft that could be converted into a military missile carrying interceptor. The baseline passenger version has a flight crew of two with cabin space for four rows of two passenger seats plus baggage and lavatory room in the aft cabin. The ramp weight is 61,600 pounds with an internal fuel capacity of 30,904 pounds. Utilizing an improved version of a current technology low-bypass ratio turbofan engine, range is 3,622 nautical miles at Mach 2.0 cruise and standard day operating conditions. Balanced field takeoff distance is 6,600 feet and landing distance is 5,170 feet at 44,737 pounds. The passenger section from aft of the flight crew station to the aft pressure bulkhead in the cabin was modified for the interceptor version. Bomb bay type doors were added and volume is sufficient for four advanced air-to-air missiles mounted on a rotary launcher. Missile volume was based on a Phoenix type missile with a weight of 910 pounds per missile for a total payload weight of 3,640 pounds. Structural and equipment weights were adjusted and result in a ramp weight of 63,246 pounds with a fuel load of 30,938 pounds. Based on a typical intercept mission flight profile, the resulting radius is 1,609 nautical miles at a cruise Mach number of 2.0.

  12. Calibration of Seismic Sources during a Test Cruise with the new RV SONNE

    Science.gov (United States)

    Engels, M.; Schnabel, M.; Damm, V.

    2015-12-01

    During autumn 2014, several test cruises of the brand new German research vessel SONNE were carried out before the first official scientific cruise started in December. In September 2014, BGR conducted a seismic test cruise in the British North Sea. RV SONNE is a multipurpose research vessel and was also designed for the mobile BGR 3D seismic equipment, which was tested successfully during the cruise. We spend two days for calibration of the following seismic sources of BGR: G-gun array (50 l @ 150 bar) G-gun array (50 l @ 207 bar) single GI-gun (3.4 l @ 150 bar) For this experiment two hydrophones (TC4042 from Reson Teledyne) sampling up to 48 kHz were fixed below a drifting buoy at 20 m and 60 m water depth - the sea bottom was at 80 m depth. The vessel with the seismic sources sailed several up to 7 km long profiles around the buoy in order to cover many different azimuths and distances. We aimed to measure sound pressure level (SPL) and sound exposure level (SEL) under the conditions of the shallow North Sea. Total reflections and refracted waves dominate the recorded wave field, enhance the noise level and partly screen the direct wave in contrast to 'true' deep water calibration based solely on the direct wave. Presented are SPL and RMS power results in time domain, the decay with distance along profiles, and the somehow complicated 2D sound radiation pattern modulated by topography. The shading effect of the vessel's hull is significant. In frequency domain we consider 1/3 octave levels and estimate the amount of energy in frequency ranges not used for reflection seismic processing. Results are presented in comparison of the three different sources listed above. We compare the measured SPL decay with distance during this experiment with deep water modeling of seismic sources (Gundalf software) and with published results from calibrations with other marine seismic sources under different conditions: E.g. Breitzke et al. (2008, 2010) with RV Polarstern

  13. Fatigue of titanium alloys in a supersonic-cruise airplane environment

    Science.gov (United States)

    Imig, L. A.

    1976-01-01

    The test programs conducted by several aerospace companies and NASA, summarized in this paper, studied several titanium materials previously identified as having high potential for application to supersonic cruise airplane structures. These studies demonstrate that the temperature (560 K) by itself produced no significant degradation of the materials. However, the fatigue resistance of titanium-alloy structures, in which thermal and loading effects are combined, has been studied insufficiently. The predominant topic for future study of fatigue problems in Mach 3 structures should be the influences of thermal stress particularly, the effects of thermal stress on failure location.

  14. Thermal management for a Mach 5 cruise aircraft using endothermic fuel

    Science.gov (United States)

    Petley, Dennis H.; Jones, Stuart C.

    1990-01-01

    The present thermal management system for a carrier-based Mach 5 cruise-capable aircraft whose propulsion system does not entail cryogenic fuels is predicated on the use of the catalytic endothermic reaction of a petroleum-derived hydrocarbon fuel as the heat sink for engine cooling. The insulation of engine flowpath surfaces reduces cooling requirements. The primary elements of this closed-cycle cooling system are a fuel preheater, a catalytic fuel reactor, and engine wall-cooling panels; a silicone-based liquid polymer is used as the coolant. Structural, weight, and thermal analysis results are presented for each of the primary components.

  15. Engaging Middle School Students in Authentic Research based on a summer research cruise

    Science.gov (United States)

    Manley, J.; Ellins, K. K.; Conte, M. H.

    2011-12-01

    In summer 2010, as a participant in the TXESS Revolution, a National Science Foundation (NSF)-sponsored professional development program for teachers in support of Earth and Space Science, I participated in a scientific research cruise led by Dr. Maureen Conte of the Bermuda Institute of Ocean Sciences (BIOS). The primary purpose of the cruise was to collect water samples from different ocean depths, make temperature and conductivity measurements, and retrieve biologic particle debris collection equipment deployed as part of the NSF-sponsored Oceanic Flux Program to measure particle fluxes in the deep Sargasso Sea. A secondary objective involved the collection of plastic debris floating within the sargassum grass trapped in the North Atlantic gyre in order to investigate plastic pollution. As a member of the science team I worked alongside of Dr. Conte, scientists and graduate students, giving me a personal experience to inspire my students' interest in the marine ecosystem. In the classroom, I used a Project Based Learning (PBL) approach to translate my experience and knowledge gained into productive learning for my students. With Project Based Learning, teams of students solve a real world, open-ended challenge problem through research and experimentation. In this Problem, the challenge was to design a virtual product to motivate ordinary people to change their habits regarding their use and improper disposal of plastics. Team products included websites, social network pages, and in-school announcements to create awareness about plastic pollution in the ocean. Fulfilling one of the basic principles of the PBL approach to provide student access to experts, cruise participant and University of North Carolina graduate student Bonnie Monteleone dedicated an entire day to speak with each of my classes about her experiences studying ocean plastics and answer their questions via SKYPE. In addition, Ms. Monteleone used her extensive contacts to post the best of my

  16. The application of dual fuel /JP-LH2/ for hypersonic cruise vehicles

    Science.gov (United States)

    Weidner, J. P.

    1978-01-01

    The possibility of utilizing jet fuel (JP) stored primarily in the wings of hydrogen-fueled hypersonic cruise vehicles has been evaluated and compared to the performance of all hydrogen-fueled aircraft. Parametric investigations of wing loading, thrust-to-weight ratio, payload size and vehicle size are presented. Results indicate improvements in performance for a wide range of potential payload sizes, particularly when in-flight refueling of the JP fuel is considered as a means of increasing range and mission flexibility.

  17. Dental practice during a world cruise: treatment needs and demands of crew.

    Science.gov (United States)

    Sobotta, Bernhard A J; John, Mike T; Nitschke, Ina

    2007-01-01

    To describe dental treatment needs and demands of crew on a cruise ship during a world cruise. The routine dental documentation of a two months period at sea on a cruise ship carrying 999 crew was analysed. Age, gender, diagnosis, treatment performed, percentage of emergency and routine procedures, number of appointments, duration of appointment and time since last visit to the dentist were recorded. Oral health-related quality of life (OHRQoL) was measured using the 14-item Oral Health Impact Profile. Subjects were n = 56 crew with a mean age of 37 (+/- 12.0) years. Out of 114 patient contacts n = 29 (25%) were for emergency treatment. Caries and its sequelae accounted for 85% of time spent treating emergencies and 50% of routine treatment time. The two most frequent treatment options during emergency appointments were extractions and endodontics. In routine cases fillings and periodontal treatment were dominating. Per 1000 persons per month crew required 14.5 emergency plus 42.5 routine appointments. 80 % of crew had seen a dentist within 12 months before their shipboard dental appointment. Oral health-related quality of life was most impaired in case of emergency patients with a particular emphasis on the diagnosis of pulpal disease. High numbers of dental emergencies largely due to caries indicated that International Labour Organisation (ILO) recommendations requiring seafarers to be dentally fit were not adhered to. It is suggested that some doctors performing pre-sea medical examinations may not adequately diagnose caries. A pre-sea examination by a dental professional has the potential to reduce the number of emergency port referrals to dentists. Treatment costs and attitude to preventive dental care were identified as barriers impeding the access of low-wage crew to the ship's dental clinic. Cosmetic dentistry and prophylaxis attracted those crew with an interest in prevention and the ability to pay the fees. In large cruise ships there is a substantial

  18. Computer-aided methods for analysis and synthesis of supersonic cruise aircraft structures

    Science.gov (United States)

    Giles, G. L.

    1976-01-01

    Computer-aided methods are reviewed which are being developed by Langley Research Center in-house work and by related grants and contracts. Synthesis methods to size structural members to meet strength and stiffness (flutter) requirements are emphasized and described. Because of the strong interaction among the aerodynamic loads, structural stiffness, and member sizes of supersonic cruise aircraft structures, these methods are combined into systems of computer programs to perform design studies. The approaches used in organizing these systems to provide efficiency, flexibility of use in an iterative process, and ease of system modification are discussed.

  19. The application of process integration to the optimisation of cruise ship energy systems: a case study

    DEFF Research Database (Denmark)

    Baldi, Francesco; Nguyen, Tuong-Van; Ahlgren, Fredrik

    2016-01-01

    and its integration with available sources of waste heat on board.In this study, the principles of process integration are applied to the energy system of a cruise ship operating in the Baltic Sea. The heat sources (waste heat from the main and auxiliary engines in form of exhaust gas, cylinder cooling......” scenario and one in the “design” scenario, with a reduction of 13-33%, 15-27% and 46-56% of the external heat demand, respectively. Given the high amount of heat being available after the process integration, we also analysed the potential for the installation of a steam turbine for the recovery...

  20. Individualizing a capstone project: a cruise ship nurse creates a brochure about the Norwalk virus.

    Science.gov (United States)

    Campbell, William T; Benn, Sonja

    2011-01-01

    An MS capstone is a summative academic project where the student is expected to combine personal experience, previous nursing knowledge, and newly acquired graduate nursing skills and integrate them into practice. The authors discuss the challenges of a capstone project from the committee chair and student perspectives. This family nurse practitioner student was a cruise ship nurse who identified a need related to the Norwalk virus. The committee chair needed to direct the student through the capstone formal process while helping her to create a professional brochure to educate future travelers.

  1. Onshore catering increases the risk of diarrhoeal illness amongst cruise ship passengers.

    Science.gov (United States)

    Pugh, R E; Selvey, L; Crome, M; Beers, M

    2001-01-01

    Of 134 Queensland passengers on a cruise, 91 (67.9%) people reported various illnesses including 41 (30.6%) who reported diarrhoeal symptoms. Queensland passengers who ate while onshore at non-Australian ports were significantly more at risk of developing diarrhoeal symptoms than those who did not. Passengers were particularly at risk when they ate onshore while undertaking a tour compared with those who did not undertake this tour. Travellers should be warned of the possibility of contracting diarrhoeal illness from onshore catering.

  2. A study of the prediction of cruise noise and laminar flow control noise criteria for subsonic air transports

    Science.gov (United States)

    Swift, G.; Mungur, P.

    1979-01-01

    General procedures for the prediction of component noise levels incident upon airframe surfaces during cruise are developed. Contributing noise sources are those associated with the propulsion system, the airframe and the laminar flow control (LFC) system. Transformation procedures from the best prediction base of each noise source to the transonic cruise condition are established. Two approaches to LFC/acoustic criteria are developed. The first is a semi-empirical extension of the X-21 LFC/acoustic criteria to include sensitivity to the spectrum and directionality of the sound field. In the second, the more fundamental problem of how sound excites boundary layer disturbances is analyzed by deriving and solving an inhomogeneous Orr-Sommerfeld equation in which the source terms are proportional to the production and dissipation of sound induced fluctuating vorticity. Numerical solutions are obtained and compared with corresponding measurements. Recommendations are made to improve and validate both the cruise noise prediction methods and the LFC/acoustic criteria.

  3. Keep on cruising: Changes in lifestyle and driving style among male drivers between the age of 18 and 23

    DEFF Research Database (Denmark)

    Møller, Mette; Haustein, Sonja

    2013-01-01

    Despite recent improvements in general road safety levels, young male drivers in most western countries continue to be overrepresented in road traffic accidents. Lifestyle related motivational factors are a key element in the young male driver problem. Based on 379 posted questionnaires completed...... by the same male drivers at the age of 18 and again at the age of 23, this study examined changes in the relationship between lifestyle and driving style over a 5 year period. A number of changes in car use, driving style and engagement in different leisure time activities were found. Cruising was related...... to an extrovert social life as well as problem behaviours such as drink driving. At the age of 18 cruising was a part of the normal social life of the majority of the participants. However, while most drivers reduced their level of cruising as well as related problem behaviour over time, a smaller group still...

  4. Temperature, salinity, nutrients, freons, oxygen, currents (ADCP), underway and other measurements collected in the Gulf of Mexico and Atlantic as part of the Gulf of Mexico and East Coast Carbon Cruise (GOMECC) 2007 (NCEI Accession 0066603)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — GOMECC Gulf of Mexico and East Coast Carbon Cruise(RB 07-05). North American Carbon Program (NACP) Gulf of Mexico and East Coast Carbon (GOMECC) Cruise on board NOAA...

  5. Physical, chemical and biological CTD and bottle data from R/V Thomas G. Thompson cruise TN278 in eastern tropical North Pacific Ocean from March 19 to April 20, 2012 (NODC Accession 0109846)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This report contains data from R/V Thomas G. Thompson cruise TN278 to the eastern tropical north pacific oxygen deficient zone. The objective of the cruise was to...

  6. Nutrients, chlorophyll, and other data from Northeast Water Column Monitoring cruises in the Mid-Atlantic Bight for the Northeast Monitoring Program (NEMP), 21 April 1980 to 24 April 1984 (NODC Accession 8800171)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Multiple cruise reports for the Northeast Monitoring Program (NEMP) describe the data collection activities, analyses and tabular data from multiple NEMP cruises in...

  7. Atmosphere-ocean ozone fluxes during the TexAQS 2006, STRATUS 2006, GOMECC 2007, GasEx 2008, and AMMA 2008 cruises

    NARCIS (Netherlands)

    Helmig, D.; Lang, E.K.; Bariteau, L.; Boylan, P.; Fairall, C.W.; Ganzeveld, L.N.; Hare, J.E.; Hueber, J.; Pallandt, M.

    2012-01-01

    A ship-based eddy covariance ozone flux system was deployed to investigate the magnitude and variability of ozone surface fluxes over the open ocean. The flux experiments were conducted on five cruises on board the NOAA research vessel Ronald Brown during 2006-2008. The cruises covered the Gulf of

  8. Atmosphere-ocean ozone fluxes during the TexAQS 2006, STRATUS 2006, GOMECC 2007, GasEx 2008, and AMMA 2008 cruises

    NARCIS (Netherlands)

    Helmig, D.; Lang, E.K.; Bariteau, L.; Boylan, P.; Fairall, C.W.; Ganzeveld, L.N.; Hare, J.E.; Hueber, J.; Pallandt, M.

    2012-01-01

    A ship-based eddy covariance ozone flux system was deployed to investigate the magnitude and variability of ozone surface fluxes over the open ocean. The flux experiments were conducted on five cruises on board the NOAA research vessel Ronald Brown during 2006-2008. The cruises covered the Gulf of M

  9. Cruise-based Multi-factorial Investigation of the Impact of Ocean Acidification on the Pelagic Biosphere

    Science.gov (United States)

    Young, J. R.; Tyrell, T.

    2012-12-01

    The pelagic ecosystem is a critical component of the earth's biosphere and biogeochemistry. It is also, however, a complex and in many respects poorly understood system. In consequence predicting the likely impact of ocean acidification on the pelagic realm is problematic and predicting the possible secondary biogeochemical effects of these impacts is "challenging". Nonetheless there is a major societal need to predict these impacts and outcomes. Within the UK Ocean Acidification Programme our consortium is tasked with "improving the understanding of the impact of ocean acidification on surface ocean biology, community structure, biogeochemistry and on feedbacks to the climate." To ensure complimentarity with other programmes we have adopted a cruise-based approach. Two cruises have been undertaken; Cruise D366 in summer 2011 around the north west european shelf and Cruise JR271 summer 2012 to the Arctic Ocean. A final cruise, to the Antarctic will be undertaken in January/February 2013. On each cruise we are combining extensive environmental observations, with deck-board incubation experiments. The environmental observations are being made with both continuous sampling techniques and CTD sampling. The cruise tracks have been designed to cross environmental gradients in ocean chemistry and especially in carbonate chemistry. The objective here is to produce a high quality matrix of multiple environmental parameters including fully characterised carbonate chemistry (pH, CO2, DIC and alkalinity are all measured), nutrient chemistry, trace elements, climatically active gases, and TEP, phytoplankton and zooplankton composition and biocalcification. The biocalcification studies include microfabric study of pteropods, in situ calcification rates and integrated morphometric and assemblage composition analysis of coccolithophores. The incubation experiments are being conducted using a dedicated culture facility constructed in a shipping-container lab. This allows large

  10. Inlet-engine matching for SCAR including application of a bicone variable geometry inlet. [Supersonic Cruise Aircraft Research

    Science.gov (United States)

    Wasserbauer, J. F.; Gerstenmaier, W. H.

    1978-01-01

    Airflow characteristics of variable cycle engines (VCE) designed for Mach 2.32 can have transonic airflow requirements as high as 1.6 times the cruise airflow. This is a formidable requirement for conventional, high performance, axisymmetric, translating centerbody mixed compression inlets. An alternate inlet is defined where the second cone of a two cone centerbody collapses to the initial cone angle to provide a large off-design airflow capability, and incorporates modest centerbody translation to minimize spillage drag. Estimates of transonic spillage drag are competitive with those of conventional translating centerbody inlets. The inlet's cruise performance exhibits very low bleed requirements with good recovery and high angle of attack capability.

  11. Disturbance decoupling robust control of vehicle full speed cruise dynamic system

    Institute of Scientific and Technical Information of China (English)

    BIN Yang; LI KeQiang; FENG NengLian

    2009-01-01

    In this study,an innovative dynamics model of LFS(longitudinal vehicle full-speed cruise system)is developed by lumping the dynamics of a controlled vehicle and an inter-vehicles together.On account of the external disturbance,parameters uncertainty and the nonlinearity within LFS,a DDRC(disturbance decoupling robust control)method is proposed.For this method,the theory of NDD(nonlinear disturbance decoupling)is utilized firstly to separate the external disturbance from certain part of the proposed dynamics model.Then,the invariance over the sliding mode of VSC(variable structure control)is used to eliminate the influence of remaining uncertain part.Finally,the DDRC method is adopted to design an LFS ACC(adaptive cruise control)system,and some numerical simulations are carried out to validate its performance.The simulation results demonstrate that the proposed control system not only exhibits an expected dynamic response,high tracking accuracy and a strong robustness,but also achieves a global optimization by means of a simplified control structure.

  12. The Asphalt Ecosystem of the Gulf of Mexico: Results From the Chapopote III Cruise

    Science.gov (United States)

    MacDonald, I. R.; Escobar, E.; Naehr, T.; Joye, S.; Spiess, V.; Cruise Participants, C.

    2007-12-01

    The Campeche Knolls region of the southern Gulf of Mexico contains numerous diapiric mounds and ridges that are associated with persistent oil slicks and extensive flows of solidified asphalt. Previous investigations1 have documented chemosynthetic communities, gas hydrates, and bubble streams that rise hundreds of meters into the water column. However, only a few of the potential features had previously been investigated. The team2 selected a series of potential sites based on satellite images of oil slicks and geophysical data. These sites were surveyed during a cruise with the Mexican ship JUSTO SIERRA during 11-24 September 2007. Visual surveys using video and digital images were completed over geophysical anomalies. Multi-corer samples of the sediments were then collected based on the visual data. A narrow beam acoustic profiler was used to search for bubble streams. Preliminary results of this expedition are presented. 1 MacDonald, I. R., et al. (2004). "Asphalt volcanism and chemosynthetic life, Campeche Knolls, Gulf of Mexico." Science 304: 999-1002. 2 Chapopote III cruise participants: J. Adams, L. F. Alvarez, J. Bliss, M. Bowells, F. Deng, E. Escobar, O. Garcia, M. M. L. Garduño, A. Gassner, K. Hunter, B. B. Jimenez, A. Leiva, I. MacDonald, T. Naehr, D. Prouty, V. Samarkin, and J. Wood.

  13. Direct pollution cost assessment of cruising tourism in the Croatian Adriatic

    Directory of Open Access Journals (Sweden)

    Hrvoje Carić

    2010-06-01

    Full Text Available Cruise tourism is one of the fastest growing sectors of the tourism industry and one that has significant environmental, economic and social impacts on target destinations. Yet, tourism decision makers, developers and managers rarely incorporate or estimate environmental impacts in their tourism development planning. Indeed, the analysis of the resulting resource exploitation is rarely undertaken until carrying capacity is breached and attractiveness diminished. In this article an assessment is offered that determines, quantifies and financially estimates emissions and waste streams so they can be compared with the direct income generated to the local economy by cruising tourism. It is applied to the Croatian part of the Adriatic and financially evaluates environmental impacts, arguing that they are negative externalities due to inappropriate internalization and management.The purpose of the assessment is to give a “snapshot” of the situation, and also to create the groundwork for a model that will assist decision makers and stakeholders, at different levels and of different interests, to prevent and reduce the ecological, health and economic risks associated with dead-end tourism development.

  14. Design and Implementation of Fuzzy Approximation PI Controller for Automatic Cruise Control System

    Directory of Open Access Journals (Sweden)

    Pallab Maji

    2015-01-01

    Full Text Available Fuzzy logic systems have been widely used for controlling nonlinear and complex dynamic systems by programming heuristic knowledge. But these systems are computationally complex and resource intensive. This paper presents a technique of development and porting of a fuzzy logic approximation PID controller (FLAC in an automatic cruise control (ACC system. ACC is a highly nonlinear process and its control is trivial due to the large change in parameters. Therefore, a suitable controller based on heuristic knowledge will be easy to develop and provide an effective solution. But the major problem with employing fuzzy logic controller (FLC is its complexity. Moreover, the designing of Rulebase requires efficient heuristic knowledge about the system which is rarely found. Therefore, in this paper, a novel rule extraction process is used to derive a FLAC. This controller is then ported on a C6748 DSP hardware with timing and memory optimization. Later, it is seamlessly connected to a network to support remote reconfigurability. A performance analysis is drawn based on processor-in loop test with Simulink model of a cruise control system for vehicle.

  15. Fundamental Aeronautics Program: Overview of Propulsion Work in the Supersonic Cruise Efficiency Technical Challenge

    Science.gov (United States)

    Castner, Ray

    2012-01-01

    The Supersonics Project, part of NASA's Fundamental Aeronautics Program, contains a number of technical challenge areas which include sonic boom community response, airport noise, high altitude emissions, cruise efficiency, light weight durable engines/airframes, and integrated multi-discipline system design. This presentation provides an overview of the current (2012) activities in the supersonic cruise efficiency technical challenge, and is focused specifically on propulsion technologies. The intent is to develop and validate high-performance supersonic inlet and nozzle technologies. Additional work is planned for design and analysis tools for highly-integrated low-noise, low-boom applications. If successful, the payoffs include improved technologies and tools for optimized propulsion systems, propulsion technologies for a minimized sonic boom signature, and a balanced approach to meeting efficiency and community noise goals. In this propulsion area, the work is divided into advanced supersonic inlet concepts, advanced supersonic nozzle concepts, low fidelity computational tool development, high fidelity computational tools, and improved sensors and measurement capability. The current work in each area is summarized.

  16. Fundamental Aeronautics Program: Overview of Project Work in Supersonic Cruise Efficiency

    Science.gov (United States)

    Castner, Raymond

    2011-01-01

    The Supersonics Project, part of NASA?s Fundamental Aeronautics Program, contains a number of technical challenge areas which include sonic boom community response, airport noise, high altitude emissions, cruise efficiency, light weight durable engines/airframes, and integrated multi-discipline system design. This presentation provides an overview of the current (2011) activities in the supersonic cruise efficiency technical challenge, and is focused specifically on propulsion technologies. The intent is to develop and validate high-performance supersonic inlet and nozzle technologies. Additional work is planned for design and analysis tools for highly-integrated low-noise, low-boom applications. If successful, the payoffs include improved technologies and tools for optimized propulsion systems, propulsion technologies for a minimized sonic boom signature, and a balanced approach to meeting efficiency and community noise goals. In this propulsion area, the work is divided into advanced supersonic inlet concepts, advanced supersonic nozzle concepts, low fidelity computational tool development, high fidelity computational tools, and improved sensors and measurement capability. The current work in each area is summarized.

  17. SeaWiFS Postlaunch Technical Report Series. Volume 2; AMT-5 Cruise Report

    Science.gov (United States)

    Hooker, Stanford B. (Editor); Firestone, Elaine R. (Editor); Aiken, James; Cummings, Denise G.; Gibb, Stuart W.; Rees, Nigel W.; Woodd-Walker, Rachel; Woodward, E. Malcolm S.; Woolfenden, James; Berthon, Jean-Francois; Dempsey, Cyril D.; Suggett, David J.; Wood, Peter; Donlon, Craig; Gonzalez-Benitez, Natalia; Huskin, Ignacio; Quevedo, Mario; Barciela-Fernandez, Rosa; deVargas, Colomban; McKee, Connor

    1998-01-01

    This report documents the scientific activities on board the Royal Research Ship (RRS) James Clark Ross (JCR) during the fifth Atlantic Meridional Transect (AMT-5), 14 September to 17 October 1997. There are three objectives of the AMT Program. The first is to derive an improved understanding of the links between biogeochemical processes, biogenic gas exchange, air-sea interactions, and the effects on, and responses of, oceanic ecosystems to climate change. The second is to investigate the functional roles of biological particles and processes that influence ocean color in ecosystem dynamics. The Program relates directly to algorithm development and the validation of remotely-sensed observations of ocean color. Because the Sea-viewing Wide Field-of-view Sensor (SeaWiFS) instrument achieved operational status during the cruise (on 18 September), AMT-5 was designated the SeaWiFS Atlantic Characterization Experiment (SeaACE) and was the only major research cruise involved in the validation of SeaWiFS data during the first 100 days of operations. The third objective involved the near-real time reporting of in situ light and pigment observations to the SeaWiFS Project, so the performance of the satellite sensor could be determined.

  18. Reducing The Climate Change Impact of Aviation By Restricting Cruise Altitudes

    Science.gov (United States)

    Williams, V.; Noland, R. B.; Toumi, R.

    Aviation has the potential to influence the global climate. The total annual fuel burned by aircraft contributes a small but significant proportion of global anthropogenic car- bon dioxide emissions, with this proportion set to increase with the projected rise in demand for air travel. Additionally, the emission of nitrogen oxides at altitude can have a much greater impact on ozone, and in turn on the atmospheric radiation bud- get, than emission at the surface. A third mechanism for the influence of aviation on climate is through the production of contrails. Contrails cover 0.1 Cruise altitude restrictions could severely constrain air space capacity, especially in parts of Europe. In addition, carbon emissions may increase due to less efficient air- craft operation at lower cruise altitudes, which would offset some of the benefits of eliminating contrail formation. An analysis of these trade-offs is presented, using the RAMS air space simulation model as applied to European airspace. This model sim- ulates the flight paths and altitudes of each aircraft and is here used to calculate emis- sions of carbon dioxide and changes in the journey time. Our analysis demonstrates that altitude restrictions on commercial aircraft could be an effective means of re- ducing climate change impacts, though it will be necessary to mitigate the increased controller workload conflicts that this will generate.

  19. The Teachers at Sea program during the CIRCEA cruise of the R/V Marion Dufresne

    Science.gov (United States)

    Laj, Carlo; Sanchez Morante, Ana; Kissel, Catherine; Leau, Hélène

    2015-04-01

    With the support of the French Polar Institute (IPEV) and EGU, two teachers from Spain and France were able to participate to the CIRCEA (CIRCulation in East Asian seas) cruise of the R/V Marion Dufresne in the South China Sea, departing and arriving in Singapore. On board, the two teachers participated to all the scientific activities, including together with the scientists and technicians, to two 4-hours shifts per day (8h total per day). During these shifts, they were involved in every step of the process of obtaining the cores, cutting, opening and labelling them, archiving, and measuring some of the physical parameters, and finally sediment description. It was possible to establish almost daily reports of the scientific progress of the cruise and to send regular logs to the participating land-based teachers in different schools mainly in Europe and in the USA, taking advantage of a list of addresses of teachers having participated to the Geosciences Information for teachers (GIFT) workshops of the European Geosciences Union. This should bring authentic science in the classroom, and indeed we received enthusiastic responses from many teachers. Also, a video report of the different aspects of the life on board was realized by Ana Morante (the teacher from Spain) and Carlo Laj (Chair of the Committee on Education of EGU) which will be shown during the GIFT workshop and during the General Assembly of EGU at the IPEV booth.

  20. Cruise report for A1-02-SC southern California CABRILLO project, Earthquake Hazards Task

    Science.gov (United States)

    Normark, William R.; Fisher, Michael A.; Gutmacher, Christina E.; Sliter, Ray; Hibbeler, Lori; Feingold, Beth; Reid, Jane A.

    2003-01-01

    A two-week marine geophysical survey obtained sidescan-sonar images and multiple sets of high-resolution seismic-reflection profiles in the southern California offshore area between Point Arguello and Point Dume. The data were obtained to support two project activities of the United States Geological Survey (USGS) Coastal and Marine Geology (CMG) Program: (1) the evaluation of the geologic hazards posed by earthquake faults and landslides in the offshore areas of Santa Barbara Channel and western Santa Monica Basin and (2) determine the location of active hydrocarbon seeps in the vicinity of Point Conception as part of a collaborative study with the Minerals Management Service (MMS). The 2002 cruise, A1-02- SC, is the fourth major data-collection effort in support of the first objective (Normark et al., 1999a, b; Gutmacher et al., 2000). A cruise to obtain sediment cores to constrain the timing of deformation interpreted from the geophysical records is planned for the summer of 2003.