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Sample records for two-span continuous cip

  1. 两跨连续斜交梁桥振动台试验研究%Shaking table tests for a two-span continuous skew girder bridge

    Institute of Scientific and Technical Information of China (English)

    许永吉; 卓卫东; 孙颖

    2016-01-01

    The dynamic characteristics and three-dimensional seismic response of a two-span continuous skew girder bridge were analyzed,using parameters,such as,skew angle,shear span ratio,reinforcement ratio and axial compression ratio and so on.Taking a skewed girder bridge of Fujian as an engineering example,a 1 ∶5 scale two-span continuous skewed girder bridge model was designed for shaking table tests.Adopting shaking table tests and FE numerical analysis, the seismic responses and damage characteristics of the skewed girder bridge were studied.Results of shaking table tests showed that the structure's acceleration response and displacement response are guite different under different types of ground motion and at the same site or different types of ground motion and at different sites;under the same skew angle. the smaller the axial compression ratio,the larger the acceleration and displacement responses;when the axial compression ratio is the same,the smaller the skew angle,the larger the acceleration and displacement responses;a reasonable choice of skew angle has a great influence on the dynamic performance of skew bridges in the design of skew bridges.%选取了斜交角、配箍率和轴压比等设计参数,对两跨连续斜交梁桥进行结构动力特性分析和单向地震动输入下结构的地震反应分析。以福建省高速公路某座斜交梁桥为工程背景,设计制作了一座1/5缩尺两跨连续混凝土斜交梁桥试验模型,结合振动台模型试验和数值模拟分析,研究斜交梁桥地震响应及震害特点。振动台模型试验结果表明:不同场地类别的地震动及同一场地类别但不同的地震动作用下,结构的加速度响应、位移响应差别较大;在相同斜交角时,轴压比越小,加速度和位移响应越大;当轴压比相同时,斜交角越小,加速度和位移响应越大。从试验可知,在斜交桥设计中,合理选择斜交角对桥梁动力性能有很大的影响。

  2. CIPS Validation Data Plan

    Energy Technology Data Exchange (ETDEWEB)

    Nam Dinh

    2012-03-01

    This report documents analysis, findings and recommendations resulted from a task 'CIPS Validation Data Plan (VDP)' formulated as an POR4 activity in the CASL VUQ Focus Area (FA), to develop a Validation Data Plan (VDP) for Crud-Induced Power Shift (CIPS) challenge problem, and provide guidance for the CIPS VDP implementation. The main reason and motivation for this task to be carried at this time in the VUQ FA is to bring together (i) knowledge of modern view and capability in VUQ, (ii) knowledge of physical processes that govern the CIPS, and (iii) knowledge of codes, models, and data available, used, potentially accessible, and/or being developed in CASL for CIPS prediction, to devise a practical VDP that effectively supports the CASL's mission in CIPS applications.

  3. Research on Hybrid Seismic Response Control System for Motion Control of Two Span Bridge

    Science.gov (United States)

    Heo, G.; Kim, C.; Jeon, S.; Seo, S.; Jeon, J.

    2016-09-01

    In this paper, a hybrid seismic response control (HSRC) system was developed to control bridge motion caused by seismic load. It was aimed at optimum vibration control, composed of a rubber bearing of passive type and a MR-damper of semi-active type. The bridge model was built for experiment, a two-span bridge of 8.3 meters in length with the HSRC system put up on it. Then, inflicting El-centro seismic load on it, shaking table tests were carried out to confirm the system's validity. The experiments were conducted under the basic structure state (without an MR-damper applied) first, and then under the state with an MR-damper applied. It was also done under the basic structure state with a reinforced rubber bearing applied, then the passive on/off state of the HSRC system, and finally the semi-active state where the control algorithm was applied to the system. From the experiments, it was observed that collision rather increased when the MR-damper alone was applied, and also that the application of the HSRC system effectively prevented it from occurring. As a result, the HSRC system was proven to be effective in mitigating responses of the two-span bridge under seismic load.

  4. Dispersion management for nonlinearity mitigation in two-span 28 GBaud QPSK phase-sensitive amplifier links.

    Science.gov (United States)

    Astra, Egon; Olsson, Samuel L I; Eliasson, Henrik; Andrekson, Peter A

    2017-06-12

    We present an investigation of dispersion map optimization for two-span single-channel 28 GBaud QPSK transmission systems with phase-sensitive amplifiers (PSAs). In experiments, when the PSA link is operated in a highly nonlinear regime, a 1.4 dB error vector magnitude (EVM) improvement is achieved compared to a one-span optimized dispersion map link due to improved nonlinearity mitigation. The two-span optimized dispersion map of a PSA link differs from the optimized dispersion map of a dispersion managed phase-insensitive amplifier (PIA) link. Simulations show that the performance of the two-span dispersion map optimized PSA link does not improve by residual dispersion optimization. Further, by using the two-span optimized dispersion maps repeatedly in a long-haul PSA link instead of one-span optimized maps, the maximum transmission reach can be improved 1.5 times.

  5. Damage assessment of a two-span RC slab using wavelet analysis

    Science.gov (United States)

    Zhu, X. Q.; Hao, H.

    2007-04-01

    A two-span RC slab that measures 6400mm×800mm×100mm with 3000mm spans and 200mm overhang on each end was tested to failure in the laboratory. UB sections were used as supports. The slab was designed according to the moment redistribution method with 11 N6 bars at 75mm centres for both positive and negative reinforcement. The slab was incrementally loaded at the middle of each span to different load levels to create crack damage using four-point loading. Twelve loading stages were performed with increasing maximum load level. Two load cells are used to record the static loads on left and right spans. The crack locations and lengths were monitored in addition to the displacement measurements. Four displacement transducers are located at two sides of the middle of each span to measure the deflection under the static load. Three sets of accelerometers with nine of them in each set are evenly distributed along the slab to measure the dynamic responses. The measured responses from the RC slab in different cracked damage states are analyzed using the wavelet transform (WT). The damping ratios and instantaneous frequency are extracted. The results show that the damping ratio and instantaneous frequency changes could be two good indicators of damage in the reinforced concrete structure.

  6. eCIP, eBook, and eLibrary%eCIP,eBook与eLibrary

    Institute of Scientific and Technical Information of China (English)

    陈源蒸

    2002-01-01

    @@一 eCIP,eBook与eLibrary表示了文献工作数字化的三个层面.eCIP(electronic Catalog-ing in Publication)表示文献书目数字化(自动编目),eBook表示文献自身数字化(电子书),eLibrary表示文献收藏、利用方式的数字化(数字图书馆).

  7. Experimental and Analytical Investigation of Deformations and Stress Distribution in Steel Bands of a Two-Span Stress-Ribbon Pedestrian Bridge

    Directory of Open Access Journals (Sweden)

    G. Sandovic

    2017-01-01

    Full Text Available The article is dedicated to the analysis of problems related to design of pedestrian bridges with flexible ribbon bands made of steel. The study is based on test results of a bridge model that has two spans (each with a length of five meters. A simplified analytical technique has been proposed for predicting vertical deformations of the bridge structure subjected to symmetrical or asymmetrical loading patterns. The technique also allows assessing the tension forces in the ribbons, which are very important for design of such structures. The analysis reveals the importance of the flexural rigidity of the ribbons that might cause significant redistribution of stresses within the steel bands.

  8. Development of mice without Cip/Kip CDK inhibitors

    Energy Technology Data Exchange (ETDEWEB)

    Tateishi, Yuki; Matsumoto, Akinobu; Kanie, Tomoharu [Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, Fukuoka 812-8582 (Japan); CREST, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012 (Japan); Hara, Eiji [Cancer Institute, Japanese Foundation for Cancer Research, 3-8-31 Ariake, Koto-ku, Tokyo 135-8550 (Japan); Nakayama, Keiko [Department of Developmental Genetics, Center for Translational and Advanced Animal Research, Graduate School of Medicine, Tohoku University, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575 (Japan); Nakayama, Keiichi I., E-mail: nakayak1@bioreg.kyushu-u.ac.jp [Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, Fukuoka 812-8582 (Japan); CREST, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012 (Japan)

    2012-10-19

    Highlights: Black-Right-Pointing-Pointer Mice lacking Cip/Kip CKIs (p21, p27, and p57) survive until embryonic day 13.5. Black-Right-Pointing-Pointer Proliferation of MEFs lacking all three Cip/Kip CKIs appears unexpectedly normal. Black-Right-Pointing-Pointer CDK2 kinase activity of the triple mutant MEFs is increased in G0 phase. -- Abstract: Timely exit of cells from the cell cycle is essential for proper cell differentiation during embryogenesis. Cyclin-dependent kinase (CDK) inhibitors (CKIs) of the Cip/Kip family (p21, p27, and p57) are negative regulators of cell cycle progression and are thought to be essential for development. However, the extent of functional redundancy among Cip/Kip family members has remained largely unknown. We have now generated mice that lack all three Cip/Kip CKIs (TKO mice) and compared them with those lacking each possible pair of these proteins (DKO mice). We found that the TKO embryos develop normally until midgestation but die around embryonic day (E) 13.5, slightly earlier than p27/p57 DKO embryos. The TKO embryos manifested morphological abnormalities as well as increased rates of cell proliferation and apoptosis in the placenta and lens that were essentially indistinguishable from those of p27/p57 DKO mice. Unexpectedly, the proliferation rate and cell cycle profile of mouse embryonic fibroblasts (MEFs) lacking all three Cip/Kip CKIs did not differ substantially from those of control MEFs. The abundance and kinase activity of CDK2 were markedly increased, whereas CDK4 activity and cyclin D1 abundance were decreased, in both p27/p57 DKO and TKO MEFs during progression from G{sub 0} to S phase compared with those in control MEFs. The extents of the increase in CDK2 activity and the decrease in CDK4 activity and cyclin D1 abundance were greater in TKO MEFs than in p27/p57 DKO MEFs. These results suggest that p27 and p57 play an essential role in mouse development after midgestation, and that p21 plays only an auxiliary role in

  9. Laboratory evaluation of the CIP 10 personal dust sampler.

    Science.gov (United States)

    Gero, A; Tomb, T

    1988-06-01

    The "capteur individuel de poussiere" CIP 10 personal dust sampler--developed by the Centre d'Etudes et Recherches de Charbonnages de France (CERCHAR) research organization--is a small, quiet, lightweight unit which samples at a flow rate of 10 L/min. It is a three-stage sampler, using two stages to remove nonrespirable dust particles and one stage to collect the respirable fraction. Airflow through the sampler is induced by the third stage, which is a rotating collector cup that contains a fine grade sponge. Laboratory tests were conducted in a dust chamber using aerosols of Arizona road dust, coal dust and silica dust. Aerosol concentrations measured with the CIP 10 were compared to those measured with the coal mine dust personal sampler unit used in the United States. The results of this study showed that aerosol concentrations measured with the CIP 10 were linearly related to those obtained with the coal mine dust personal sampler. The relationship, however, was dependent on preselector configuration and aerosol characteristics. The collection medium allows some small particles (less than 3 microns) to pass through the sampler without being collected. As much as 13% (by weight) of the aerosol that penetrated through the preseparating stages was exhausted from the sampler.

  10. Deficiency of G1 regulators P53, P21Cip1 and/or pRb decreases hepatocyte sensitivity to TGFβ cell cycle arrest

    Directory of Open Access Journals (Sweden)

    Harrison David J

    2007-11-01

    Full Text Available Abstract Background TGFβ is critical to control hepatocyte proliferation by inducing G1-growth arrest through multiple pathways leading to inhibition of E2F transcription activity. The retinoblastoma protein pRb is a key controller of E2F activity and G1/S transition which can be inhibited in viral hepatitis. It is not known whether the impairment of pRb would alter the growth inhibitory potential of TGFβ in disease. We asked how Rb-deficiency would affect responses to TGFβ-induced cell cycle arrest. Results Primary hepatocytes isolated from Rb-floxed mice were infected with an adenovirus expressing CRE-recombinase to delete the Rb gene. In control cells treatment with TGFβ prevented cells to enter S phase via decreased cMYC activity, activation of P16INK4A and P21Cip and reduction of E2F activity. In Rb-null hepatocytes, cMYC activity decreased slightly but P16INK4A was not activated and the great majority of cells continued cycling. Rb is therefore central to TGFβ-induced cell cycle arrest in hepatocytes. However some Rb-null hepatocytes remained sensitive to TGFβ-induced cell cycle arrest. As these hepatocytes expressed very high levels of P21Cip1 and P53 we investigated whether these proteins regulate pRb-independent signaling to cell cycle arrest by evaluating the consequences of disruption of p53 and p21Cip1. Hepatocytes deficient in p53 or p21Cip1 showed diminished growth inhibition by TGFβ. Double deficiency had a similar impact showing that in cells containing functional pRb; P21Cip and P53 work through the same pathway to regulate G1/S in response to TGFβ. In Rb-deficient cells however, p53 but not p21Cip deficiency had an additive effect highlighting a pRb-independent-P53-dependent effector pathway of inhibition of E2F activity. Conclusion The present results show that otherwise genetically normal hepatocytes with disabled p53, p21Cip1 or Rb genes respond less well to the antiproliferative effects of TGFβ. As the function of

  11. New AIM/CIPS global observations of gravity waves near 50-55 km

    Science.gov (United States)

    Randall, C. E.; Carstens, J.; France, J. A.; Harvey, V. L.; Hoffmann, L.; Bailey, S. M.; Alexander, M. J.; Lumpe, J. D.; Yue, J.; Thurairajah, B.; Siskind, D. E.; Zhao, Y.; Taylor, M. J.; Russell, J. M.

    2017-07-01

    This paper describes a new data set from the Aeronomy of Ice in the Mesosphere (AIM) Cloud Imaging and Particle Size (CIPS) instrument, from which gravity waves (GWs) at an altitude of 50-55 km can be inferred. CIPS is sensitive to GWs with horizontal wavelengths from 15 to 600 km and vertical wavelengths longer than 15 km. Several examples of GWs in CIPS observations are shown, including waves associated with the Andes Mountains, island topography, convection, the polar night jet, and the tropospheric jet stream. GW signatures in the CIPS data are shown to agree well with near-coincident but lower altitude measurements from the Atmospheric Infrared Sounder (AIRS) in June of 2016. Results suggest the power of combining CIPS measurements with those from other instruments to investigate GW filtering and propagation. The CIPS data set opens new areas of inquiry, enabling comprehensive investigations of GWs in the middle atmosphere on a near-global scale.

  12. p21WAF1/CIP1 gene DNA sequencing and its expression in human osteosarcoma

    Institute of Scientific and Technical Information of China (English)

    廖威明; 张春林; 李佛保; 曾炳芳; 曾益新

    2004-01-01

    Background Mutation and expression change of p21WAF1/CIP1 may play a role in the growth of osteosarcoma. This study was to investigate the expression of the p21WAF1/CIP1 gene in human osteosarcoma, p21WAF1/CIP1 gene DNA sequence change and their relationships with the phenotype and clinical prognosis.Methods p21WAF1/CIP1 gene in 10 normal people and the tumours of 45 osteosarcoma patients were examined using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) with silver staining. The PCR product with an abnormal strand was sequenced directly. The p21WAF1/CIP1 gene mRNA and P21 protein of 45 cases of osteosarcoma were investigated by using in situ hybridization and immunohistochemistry, respectively. Results The occurrence of P21 protein in osteosarcoma was 17.78% (8/45), and p21WAF1/CIP1 mRNA expression in osteosarcoma was 42.22% (19/45). The p21WAF1/CIP1 gene DNA sequencing of amplified production showed that in p21WAF1/CIP1 gene exon 3 of 36 cases of human osteosarcoma, there were 17 cases (47.22%) with C→T at position 609; 10 normal blood samples' DNA sequence analysis yielded 8 cases (80.00%) with C→T at the same position. Conclusions Along with the increase of malignancy, the expression of p21WAF1/CIP1mRNA and P21 protein in osteosarcoma tends to decrease. It is uncommon for the p21WAF1/CIP1 gene mutation to occur in human osteosarcoma. As a result, the possible existence of tumour subtypes of p21WAF1/CIP1 gene mutation should be investigated. Our research leads to the location of p21WAF1/CIP1 gene polymorphism of Chinese osteosarcoma patients, which can provide a basis for further research.

  13. Overexpression and small molecule-triggered downregulation of CIP2A in lung cancer.

    Directory of Open Access Journals (Sweden)

    Liang Ma

    Full Text Available BACKGROUND: Lung cancer is the leading cause of cancer deaths worldwide, with a five-year overall survival rate of only 15%. Cancerous inhibitor of PP2A (CIP2A is a human oncoprotein inhibiting PP2A in many human malignancies. However, whether CIP2A can be a new drug target for lung cancer is largely unclear. METHODOLOGY/PRINCIPAL FINDINGS: Normal and malignant lung tissues were derived from 60 lung cancer patients from southern China. RT-PCR, Western blotting and immunohistochemistry were used to evaluate the expression of CIP2A. We found that among the 60 patients, CIP2A was undetectable or very low in paratumor normal tissues, but was dramatically elevated in tumor samples in 38 (63.3% patients. CIP2A overexpression was associated with cigarette smoking. Silencing CIP2A by siRNA inhibited the proliferation and clonogenic activity of lung cancer cells. Intriguingly, we found a natural compound, rabdocoetsin B which is extracted from a Traditional Chinese Medicinal herb Rabdosia coetsa, could induce down-regulation of CIP2A and inactivation of Akt pathway, and inhibit proliferation and induce apoptosis in a variety of lung cancer cells. CONCLUSIONS/SIGNIFICANCE: Our findings strongly indicate that CIP2A could be an effective target for lung cancer drug development, and the therapeutic potentials of CIP2A-targeting agents warrant further investigation.

  14. Aves do Parque Nacional da Serra do Cipó: o Vale do Rio Cipó, Minas Gerais, Brasil The birds of "Parque Nacional da Serra do Cipó": the Rio Cipó valley, Minas Gerais, Brazil

    Directory of Open Access Journals (Sweden)

    Marcos Rodrigues

    2005-06-01

    Full Text Available Foi conduzido um levantamento de espécies de aves do Vale do alto Rio Cipó durante o período de maio de 1998 a novembro de 2002. A região está totalmente inserida em uma das unidades de conservação mais importantes do sudeste do Brasil, o Parque Nacional da Serra do Cipó, em Minas Gerais. O método utilizado foi o de observação direta ao longo de 'transectos', captura com redes e identificação a partir do uso de vocalizações. A riqueza de espécies foi estimada usando-se o método de 'jackknife'. Foram registradas 226 espécies de aves pertencentes a 43 famílias. Isso corresponde cerca de 27% das 837 espécies já registradas para o bioma do Cerrado. Foram capturados 2.249 indivíduos num total de 4.486,82 horas-rede, onde foram amostradas 119 espécies pertencentes a 23 famílias. A riqueza foi estimada em 239 ± 5 espécies. Constam nesta lista seis espécies endêmicas do Cerrado: Augastes scutatus (Temminck, 1824 (Trochilidae, Hylocryptus rectirostris (Wied-NeuWied, 1821 (Furnariidae, Antilophia galeata (Lichtenstein, 1832 (Pipridae, Cyanocorax cristatellus (Temminck, 1823 (Corvidae, Charitospiza eucosma (Oberholser, 1905, Saltator atricollis (Vieillot, 1817, e Porphyrospiza caerulescens (Wied-Neuwied, 1830 (Emberizidae. Ocorrem também três espécies quase-ameaçadas de extinção: Sarcoramphus papa (Linnaeus, 1758 (Cathartidae, Cypsnagra hirundinacea (Lesson, 1831 e Charitospiza eucosma (Emberizidae. O Vale do Rio Cipó abriga uma porção significativa da avifauna do Cerrado. Alguns dos habitat encontrados no Vale estão se tornando cada vez mais raros na região do Cerrado de todo o Brasil, como as matas ciliares e o sistema de lagoas temporárias ao longo dos rios. Mesmo as cachoeiras, habitat importante para várias espécies, vêm desaparecendo em outras regiões do Brasil. Nesse sentido, a região do Vale do Rio Cipó dentro Parque consolida um dos seus objetivos que é a conservação da biodiversidade.It is

  15. Development of an abbreviated Career Indecision Profile-65 using item response theory: The CIP-Short.

    Science.gov (United States)

    Xu, Hui; Tracey, Terence J G

    2017-03-01

    The current study developed an abbreviated version of the Career Indecision Profile-65 (CIP-65; Hacker, Carr, Abrams, & Brown, 2013) by using item response theory. In order to improve the efficiency of the CIP-65 in measuring career indecision, the individual item performance of the CIP-65 was examined with respect to the ordering of response occurrence and gender differential item functioning. The best 5 items of each scale of the CIP-65 (i.e., neuroticism/negative affectivity, choice/commitment anxiety, lack of readiness, and interpersonal conflicts) were retained in the CIP-Short using a sample of 588 college students. A validation sample (N = 174) supported the reliability and structural validity of the CIP-Short. The convergent and divergent validity of the CIP-Short was additionally supported in the findings of a hypothesized differential relational pattern in a separate sample (N = 360). While the current study supported the CIP-Short being a sound brief measure of career indecision, the limitations of this study and suggestions for future research were discussed as well. (PsycINFO Database Record

  16. Comparison of wing-span averaging effects on lift, rolling moment, and bending moment for two span load distributions and for two turbulence representations

    Science.gov (United States)

    Lichtenstein, J. H.

    1978-01-01

    An analytical method of computing the averaging effect of wing-span size on the loading of a wing induced by random turbulence was adapted for use on a digital electronic computer. The turbulence input was assumed to have a Dryden power spectral density. The computations were made for lift, rolling moment, and bending moment for two span load distributions, rectangular and elliptic. Data are presented to show the wing-span averaging effect for wing-span ratios encompassing current airplane sizes. The rectangular wing-span loading showed a slightly greater averaging effect than did the elliptic loading. In the frequency range most bothersome to airplane passengers, the wing-span averaging effect can reduce the normal lift load, and thus the acceleration, by about 7 percent for a typical medium-sized transport. Some calculations were made to evaluate the effect of using a Von Karman turbulence representation. These results showed that using the Von Karman representation generally resulted in a span averaging effect about 3 percent larger.

  17. CIP2A is a candidate therapeutic target in clinically challenging prostate cancer cell populations

    Science.gov (United States)

    Khanna, Anchit; Rane, Jayant K.; Kivinummi, Kati K.; Urbanucci, Alfonso; Helenius, Merja A.; Tolonen, Teemu T.; Saramäki, Outi R.; Latonen, Leena; Manni, Visa; Pimanda, John E.; Maitland, Norman J.; Westermarck, Jukka; Visakorpi, Tapio

    2015-01-01

    Residual androgen receptor (AR)-signaling and presence of cancer stem-like cells (SCs) are the two emerging paradigms for clinically challenging castration-resistant prostate cancer (CRPC). Therefore, identification of AR-target proteins that are also overexpressed in the cancer SC population would be an attractive therapeutic approach. Our analysis of over three hundred clinical samples and patient-derived prostate epithelial cultures (PPECs), revealed Cancerous inhibitor of protein phosphatase 2A (CIP2A) as one such target. CIP2A is significantly overexpressed in both hormone-naïve prostate cancer (HN-PC) and CRPC patients. CIP2A is also overexpressed, by 3- and 30-fold, in HN-PC and CRPC SCs respectively. In vivo binding of the AR to the intronic region of CIP2A and its functionality in the AR-moderate and AR-high expressing LNCaP cell-model systems is also demonstrated. Further, we show that AR positively regulates CIP2A expression, both at the mRNA and protein level. Finally, CIP2A depletion reduced cell viability and colony forming efficiency of AR-independent PPECs as well as AR-responsive LNCaP cells, in which anchorage-independent growth is also impaired. These findings identify CIP2A as a common denominator for AR-signaling and cancer SC functionality, highlighting its potential therapeutic significance in the most clinically challenging prostate pathology: castration-resistant prostate cancer. PMID:25965834

  18. p21WAF1/CIP1 interacts with protein kinase CK2

    DEFF Research Database (Denmark)

    Götz, C; Wagner, P; Issinger, O G

    1996-01-01

    and inhibits DNA replication. Here, we show that p21WAF1/CIP1 binds to the regulatory beta-subunit of protein kinase CK2 but not to the catalytic alpha-subunit. Binding of p21WAF1/CIP1 down regulates the kinase activity of CK2 with respect to the phosphorylation of the beta-subunit of CK2, casein and the C......p21WAF1/CIP1 which belongs to a class of regulatory proteins that interact with cyclin dependent kinases is a potent inhibitor of these kinases. The inhibition of the cyclin dependent kinases induces an arrest of cells in the G phase of the cell cycle. In addition p21WAF1/CIP1 associates with PCNA......-terminus of p53. This study demonstrates a new binding partner for the regulatory beta-subunit of protein kinase CK2 which regulates the activity of the holoenzyme....

  19. Afatinib induces apoptosis in NSCLC without EGFR mutation through Elk-1-mediated suppression of CIP2A.

    Science.gov (United States)

    Chao, Ting-Ting; Wang, Cheng-Yi; Chen, Yen-Lin; Lai, Chih-Cheng; Chang, Fang-Yu; Tsai, Yi-Ting; Chao, Chung-Hao H; Shiau, Chung-Wai; Huang, Yuh-Chin T; Yu, Chong-Jen; Chen, Kuen-Feng

    2015-02-10

    Afatinib has anti-tumor effect in non-small cell lung carcinoma (NSCLC) with epidermal growth factor receptor (EGFR) mutation. We found afatinib can also induce apoptosis in NSCLC cells without EGFR mutation through CIP2A pathway. Four NSCLC cell lines (H358 H441 H460 and A549) were treated with afatinib to determine their sensitivity to afatinib-induced cell death and apoptosis. The effects of CIP2A on afatinib-induced apoptosis were confirmed by overexpression and knockdown of CIP2A expression in the sensitive and resistant cells, respectively. Reduction of Elk-1 binding to the CIP2A promoter and suppression of CIP2A transcription were analyzed. In vivo efficacy of afatinib against H358 and H460 xenografts tumors were also determined in nude mice. Afatinib induced significant cell death and apoptosis in H358 and H441 cells, but not in H460 or A549 cells. The apoptotic effect of afatinib in sensitive cells was associated with downregulation of CIP2A, promotion of PP2A activity and decrease in AKT phosphorylation. Afatinib suppressed CIP2A at the gene transcription level by reducing the promoter binding activity of Elk-1. Clinical samples showed that higher CIP2A expression predicted a poor prognosis and Elk-1 and CIP2A expressions were highly correlated. In conclusion, afatinib induces apoptosis in NSCLC without EGFR mutations through Elk-1/CIP2A/PP2A/AKT pathway.

  20. Characterization of cells resistant to the potent histone deacetylase inhibitor spiruchostatin B (SP-B) and effect of overexpressed p21waf1/cip1 on the SP-B resistance or susceptibility of human leukemia cells.

    Science.gov (United States)

    Kanno, Syu-Ichi; Maeda, Naoyuki; Tomizawa, Ayako; Yomogida, Shin; Katoh, Tadashi; Ishikawa, Masaaki

    2012-09-01

    We previously showed that the B cell leukemia cell line NALM-6 had the highest susceptibility among a number of leukemia cell lines to spiruchostatin B (SP-B), a potent histone deacetylase (HDAC) inhibitor. We also showed that SP-B-induced cytotoxicity depended on induction of apoptosis that was mediated by p21waf1/cip1 expression. In the present study, we generated and characterized a stable, SP-B-resistant NALM-6 cell line (NALM-6/SP-B) by continuous exposure to SP-B, starting with a low SP-B concentration. NALM-6/SP-B cells were also more resistant to FK228, which has a similar chemical structure to SP-B, and were slightly more resistant to the P-gp substrates doxorubicin and vincristine than parental cells, but displayed similar susceptibility to other HDAC inhibitors and to paclitaxel as the parental cells. There was little change in the basal mRNA expression of HDAC1, p53, Bax, Bcl-2, Fas, caspase-3, c-Myc and MDR1 in NALM-6/SP-B compared to parental cells, but the mRNA expression of p21waf1/cip1 was decreased. The introduction of an exogenous p21waf1/cip1 expression vector restored SP-B induction of NALM-6/SP-B cell apoptosis. Moreover, overexpressed p21waf1/cip1 enhanced SP-B induction of the apoptosis of the human erythroleukemia leukemia cell line K562 which is less susceptible to SP-B than NALM-6 cells. These results suggest that downregulation of p21waf1/cip1, which is a characteristic feature of NALM-6/SP-B cells, was important for their resistance to SP-B, and that this SP-B resistance could be overcome by the introduction of exogenous p21waf1/cip1. Furthermore, introduction of p21waf1/cip1 to other leukemia cells such as K562 may enhance their susceptibility to SP-B. This is the first report of the characterization of SP-B-resistant cells and of the effect of overexpressed p21waf1/cip1 on the resistance or susceptibility of human leukemia cells to SP-B.

  1. Absence of prognostic significance of p21(WAF1/CIP1) protein expression in non-small cell lung cancer.

    Science.gov (United States)

    Dworakowska, Dorota; Jassem, Ewa; Jassem, Jacek; Boltze, Carsten; Wiedorn, Klaus Hermann; Dworakowski, Rafał; Skokowski, Jan; Jaśkiewicz, Kazimierz; Czestochowska, Eugenia

    2005-01-01

    Prognostic value of p21WAF1/CIP1 expression in non-small-cell lung cancer patients (NSCLC) remains unclear. In this study the authors investigated the clinical significance of p21WAF1/CIP1 expression in a group of 117 NSCLC patients, who underwent curative pulmonary resection. Expression of p21WAF1/CIP1 protein was assessed immunohistochemically and samples showing>5% of positive tumor cells were considered positive. Seventy-six samples (65%) showed positive nuclear p21WAF1/CIP1 protein expression. There was no relationship between the expression of p21WAF1/CIP1 protein and major clinico-pathological factors, and neither there was an impact of p21WAF1/CIP1 protein expression on disease-free and overall survival. p21WAF1/CIP1 protein occurrence was not correlated with previously determined p53 protein expression and there was also no relationship between all possible p21WAF1/CIP1/p53 phenotypes and survival. In uni- and multivariate analysis only stage of disease was independent prognostic factors. These results suggest the lack of prognostic relevance of p21WAF1/CIP1 expression (analyzed separately or jointly with p53 protein) in surgically treated NSCLC patients.

  2. 民用飞机两跨裂纹结构的剩余强度分析%Residual Strength Analysis about the Two-span Crack Structure of Civil Aircraft

    Institute of Scientific and Technical Information of China (English)

    许海生

    2012-01-01

    According the universal method, the residual strength of civil aircraft fuselage skin and wing skin can meet two-span crack. The designer need evaluates the residual strength of the skin in the condition of two-span crack in the primary-phrase design.%根据通用做法,民用飞机机身和机翼壁板在“两跨”长度裂纹存在情况下剩余强度能够满足要求,设计人员在飞机设计的初级阶段就要对两跨裂纹情况下壁板剩余强度进行评估.

  3. POST-TRANSCRIPTIONAL REGULATION OF P21WAF1/CIP1 BY P53

    Institute of Scientific and Technical Information of China (English)

    季加孚; 张霁; 焦春雨; 顾晋; 谭立新; 张平; 李培详

    2001-01-01

    Objective: To investigate the post-transcriptional regulation of p21WAF1/CIP1 by p53. Methods: The MDA-MB-468 cells have endogenous mutant p53 and the MCF7 cells lines have wtp53. Recombinant p53 expression and p21WAF1/CIP1 induction were detected by Western blot analysis. Northern blot analysis was carried out to examine whether changes in p21WAF1/CIP1 protein levels in MCF7 cells treated with AdCMVp53 are reflected at the mRNA level. Flow cytometric analysis of MCF7 cells following overexpression of recombination. Results: The ratio of p53: p21WAF1/CIP1 was below 1 at the early stages of AdCMVp53 infection, but increased to 1.6 by day 3 and to 9.7 by day 5 post-infection. As expected, p21WAF1/CIP1 expression was not detectable in MDA-MB-468 cells despite the presence of high levels of mutant p53 protein. The G1/S ratios in untreated controls and AdCMVβgal infected MCF7 cells were 1.10 and 1.35, respectively. By Northern blot analyzing the p21WAF1/CIP1: GAPDH ratios at different time points against the ratio at time point 0, a maximum 3-fold induction of p21WAF1/CIP1 mRNA expression relative to untreated control was observed on day 1 post-infection. The flow cytometric analysis indicated that MCF7 cells infected with AdCMVp53 undergo G1 arrest at both time points studied, with G1/S ratios ranging from 5.54 at day 1 to 5.65 at day 7. The G1/S ratios in untreated controls and AdCMVβgal infected MCF7 cells were 1.10 and 1.35, respectively. Conclusion: This studydemonstrated that p53 could regulate p21WAF1/CIP1 gene expression at both the transcriptional and post-transcriptional levels in MCF7 cells. The latter mechanism may be involved in or be responsible for, the induction of cell cycle arrest by transcription-defective mutants of p53.

  4. Cellulose hydrolysis ability of a Clostridium thermocellum cellulosome containing small-size scaffolding protein CipA.

    Science.gov (United States)

    Deng, Lan; Mori, Yutaka; Sermsathanaswadi, Junjarus; Apiwatanapiwat, Waraporn; Kosugi, Akihiko

    2015-10-20

    Mutant Clostridium thermocellum YM72 that produces small-size scaffolding protein CipA (ssCipA) was isolated from wild-type YM4. Sequencing of ssCipA revealed that two domains, cohesin 6 and cohesin 7, were not present. Cellulosome prepared from YM72 exhibited a significant reduction of hydrolysis ability on crystalline celluloses such as Sigmacell type-20 and cellulose from Halocynthia. To investigate this influence in vitro, artificial cellulosomes were assembled as recombinant CipA (rCipA) and ssCipA (rssCipA) using native free-cellulosomal subunits. The cellulosome assembled using rssCipA showed a 1.8-fold decrease in the hydrolysis of crystalline cellulose compared with that of rCipA. However, no significant differences in the hydrolysis of carboxymethylcellulose and acid-swollen cellulose were observed. One protein band was missing from the complex that was assembled using rssCipA (confirmed by native-PAGE). The missing protein was identified as CelJ, which is a major cellulosomal subunit. This suggests that insufficient cooperation of CelJ into the cellulosome results in the significant reduction of hydrolysis toward crystalline cellulose. These results indicate that cohesin 6 and 7 may be responsible for the cooperation of CelJ through cohesin and dockerin interactions, and adequate cooperation of CelJ into the cellulosome is important for significant hydrolysis of crystalline cellulose. Copyright © 2015 Elsevier B.V. All rights reserved.

  5. Expression and role of CIP2A in myelodysplastic syndromes(MDS)%CIP2A在骨髓增生异常综合症中的意义探讨

    Institute of Scientific and Technical Information of China (English)

    王石松

    2013-01-01

    Objective To investigate the expression and significance of cancerous inhibitor of protein phosphatase 2A(CIP2A) in myelodysplastic syndromes(MDS). Methods The immunohistochemical SP staining was used to detect expression of CIP2A in control group and in MDS. Result Expression of CIP2A in MDS was significantly higher than that in control group(P 0.05). CIP2A in high-risk group was significantly higher than that in low-risk group(P <0.05). CIP2A in AML group was significantly higher than that in high-risk group(P <0.05). Conclusion The expression of CIP2A is significantly increased in MDS bone marrow mononuclear cells, and the positive expression of CIP2A is associated with the genesis and prognosis of MDS.%  目的探讨CIP2A在骨髓增生异常综合症(MDS)中的表达及意义.方法采用免疫组化SP法对31例MDS患者、19例急性髓系白血病(AML)患者(包括4例由MDS转变而来)和20例对照组骨髓单个核细胞中CIP2A蛋白质水平进行检测.结果 MDS组CIP2A蛋白的表达水平高于对照组,差异有统计学意义(P <0.05).MDS高危组CIP2A蛋白的表达水平显著高于MDS低危组(P <0.05),AML组CIP2A蛋白的表达水平显著高于MDS高危组(P <0.05).结论 CIP2A在MDS骨髓单个核细胞中高表达可能与MDS的疾病发展有关.

  6. Draft Genome Sequence of the Butyric Acid Producer Clostridium tyrobutyricum Strain CIP I-776 (IFP923).

    Science.gov (United States)

    Wasels, François; Clément, Benjamin; Lopes Ferreira, Nicolas

    2016-03-03

    Here, we report the draft genome sequence of Clostridium tyrobutyricum CIP I-776 (IFP923), an efficient producer of butyric acid. The genome consists of a single chromosome of 3.19 Mb and provides useful data concerning the metabolic capacities of the strain.

  7. Draft Genome Sequence of the Butyric Acid Producer Clostridium tyrobutyricum Strain CIP I-776 (IFP923)

    OpenAIRE

    2016-01-01

    Here, we report the draft genome sequence of Clostridium tyrobutyricum CIP I-776 (IFP923), an efficient producer of butyric acid. The genome consists of a single chromosome of 3.19 Mb and provides useful data concerning the metabolic capacities of the strain.

  8. CipA of Acinetobacter baumannii Is a Novel Plasminogen Binding and Complement Inhibitory Protein.

    Science.gov (United States)

    Koenigs, Arno; Stahl, Julia; Averhoff, Beate; Göttig, Stephan; Wichelhaus, Thomas A; Wallich, Reinhard; Zipfel, Peter F; Kraiczy, Peter

    2016-05-01

    Acinetobacter baumannii is an emerging opportunistic pathogen, responsible for up to 10% of gram-negative, nosocomial infections. The global increase of multidrug-resistant and pan-resistant Acinetobacter isolates presents clinicians with formidable challenges. To establish a persistent infection,A. baumannii must overcome the detrimental effects of complement as the first line of defense against invading microorganisms. However, the immune evasion principles underlying serum resistance inA. baumannii remain elusive. Here, we identified a novel plasminogen-binding protein, termed CipA. Bound plasminogen, upon conversion to active plasmin, degraded fibrinogen and complement C3b and contributed to serum resistance. Furthermore, CipA directly inhibited the alternative pathway of complement in vitro, irrespective of its ability to bind plasminogen. A CipA-deficient mutant was efficiently killed by human serum and showed a defect in the penetration of endothelial monolayers, demonstrating that CipA is a novel multifunctional protein that contributes to the pathogenesis ofA. baumannii.

  9. Efficacy of the Social Skills Improvement System Classwide Intervention Program (SSIS-CIP) Primary Version

    Science.gov (United States)

    DiPerna, James Clyde; Lei, Puiwa; Bellinger, Jillian; Cheng, Weiyi

    2015-01-01

    A multisite cluster randomized trial was conducted to examine the effects of the Social Skills Improvement System Classwide Intervention Program (SSIS-CIP; Elliott & Gresham, 2007) on students' classroom social behavior. The final sample included 432 students across 38 second grade classrooms. Social skills and problem behaviors were measured…

  10. Development of CIP/graphite composite additives for electromagnetic wave absorption applications

    Science.gov (United States)

    Woo, Soobin; Yoo, Chan-Sei; Kim, Hwijun; Lee, Mijung; Quevedo-Lopez, Manuel; Choi, Hyunjoo

    2017-09-01

    In this study, the electromagnetic (EM) wave absorption ability of carbonyl iron powder (CIP)/graphite composites produced by ball milling were studied in a range of 28.5 GHz to examine the effects of the morphology and volume fraction of graphite on EM wave absorption ability. The results indicated that a ball milling technique was effective in exfoliating the graphite and covering it with CIP, thereby markedly increasing the specific surface area of the hybrid powder. The increase in the surface area and hybridization with dielectric loss materials (i.e., graphite) improved EM absorbing properties of CIP in the range of S and X bands. Specifically, the CIP/graphite composite containing 3 wt% graphite exhibited electromagnetic wave absorption of -13 dB at 7 GHz, -21 dB at 5.8 GHz, and -29 dB at 4.3 GHz after 1 h, 8 h, and 16 h of milling, respectively. [Figure not available: see fulltext.

  11. Computer Integrated Manufacture System (CIMS/CIPS) (to be continued)%计算机集成制造(生产)系统(CIMS/CIPS)

    Institute of Scientific and Technical Information of China (English)

    史有群; 许世范

    1999-01-01

    @@ 4 计算机网络计算模式的发展 计算机网络计算模式的发展经历了集中式大型机、PC/文件服务器、客户/服务器3个阶段,目前随着Internet和Intranet技术的发展正进入Web浏览器/服务器模式(Browser/Server)阶段.

  12. CIP4 promotes metastasis in triple-negative breast cancer and is associated with poor patient prognosis.

    Science.gov (United States)

    Cerqueira, Otto L D; Truesdell, Peter; Baldassarre, Tomas; Vilella-Arias, Santiago A; Watt, Kathleen; Meens, Jalna; Chander, Harish; Osório, Cynthia A B; Soares, Fernando A; Reis, Eduardo M; Craig, Andrew W B

    2015-04-20

    Signaling via epidermal growth factor receptor (EGFR) and Src kinase pathways promote triple-negative breast cancer (TNBC) cell invasion and tumor metastasis. Here, we address the role of Cdc42-interacting protein-4 (CIP4) in TNBC metastasis in vivo, and profile CIP4 expression in human breast cancer patients. In human TNBC cells, CIP4 knock-down (KD) led to less sustained activation of Erk kinase and impaired cell motility compared to control cells. This correlated with significant defects in 3D invasion of surrounding extracellular matrix by CIP4 KD TNBC cells when grown as spheroid colonies. In mammary orthotopic xenograft assays using both human TNBC cells (MDA-MB-231, HCC 1806) and rat MTLn3 cells, CIP4 silencing had no overt effect on tumor growth, but significantly reduced the incidence of lung metastases in each tumor model. In human invasive breast cancers, high CIP4 levels was significantly associated with high tumor stage, TNBC and HER2 subtypes, and risk of progression to metastatic disease. Together, these results implicate CIP4 in promoting metastasis in TNBCs.

  13. Expression of CIP2A in laryngeal squamous cell carcinoma and its clinical significance%CIP2A在喉癌组织中的表达及其临床意义

    Institute of Scientific and Technical Information of China (English)

    莫海兰; 李洁; 杨智玲; 李兵

    2012-01-01

    Objective To determine the role of CIP2A in the development and progression of laryngeal squamous cell carcinoma and investigate its clinical significance. Methods Expression of CIP2A was examined with immunohistochemistry in 61 tissue samples of pathologically identified laryngeal squamous cell carcinoma and their 40 adjacent normal tissues, 9 atypical hyperplasia and 8 laryngeal papilloma. The relationship between CIP2A with the clinicalpathological characteristics and the effect of CIP2A expression on prognosis were analyzed. Results The expression of CIP2A was significantly higher in laryngeal squamous cell carcinoma than atypical hyperplasia and laryngeal papilloma (P<0. 05). CIP2A immunoreactivity, tested in 61 laryngeal cancer tissues, was negative in 11 cases (18.0% ) , weakly positive in 13 cases (21.3% ) , moderately positive in 25 cases (41.0% ), and strongly positive in 12 cases (19.7% ). The expression of CIP2A was not related with age, sex, smoking and alcohol assumption. Whereas, there were significant correlations of CIP2A with T stage, clinical stage, lymph node metastasis, and histological differentiation ( P < 0. 05 ). High CIP2A expression predicted poor survival, and was associated with shorter overall-survival and disease-free survival. Conclusion Over-expression of CIP2A might be involved in the development and progression of laryngeal squamous cell carcinoma. CIP2A may serve as a useful prognostic marker and therapeutic target for laryngeal squamous cell carcinoma.%目的 探讨蛋白磷酸酶2A的癌性抑制因子(cancerous inhibitor of protein phosphatase 2A,CIP2A)在喉癌组织中的表达及其与临床病理特征的关系和对预后的影响.方法 采用免疫组化方法检测61例喉癌标本(包括40例对应癌旁正常组织),9例不典型增生和8例喉乳头状瘤中CIP2A的表达,结合临床病理资料进行分析.结果 CIP2A在正常癌旁组织中低表达或不表达,在喉鳞状细胞癌中的表达比在喉乳头

  14. Protective role of p21(Waf1/Cip1) against prostaglandin A2-mediated apoptosis of human colorectal carcinoma cells.

    Science.gov (United States)

    Gorospe, M; Wang, X; Guyton, K Z; Holbrook, N J

    1996-01-01

    Prostaglandin A2 (PGA2) suppresses tumor growth in vivo, is potently antiproliferative in vitro, and is a model drug for the study of the mammalian stress response. Our previous studies using breast carcinoma MCF-7 cells suggested that p21(Waf1/Cip1) induction enabled cells to survive PGA2 exposure. Indeed, the marked sensitivity of human colorectal carcinoma RKO cells to the cytotoxicity of PGA2 is known to be associated with a lack of a PGA2-mediated increase in p21(Waf1/Cip1) expression, inhibition of cyclin-dependent kinase activity, and growth arrest. To determine if cell death following exposure to PGA2 could be prevented by forcing the expression of p21(Waf1/Cip1) in RKO cells, we utilized an adenoviral vector-based expression system. We demonstrate that ectopic expression of p21(Waf1/Cip1) largely rescued RKO cells from PGA2-induced apoptotic cell death, directly implicating p21(Waf1/Cip1) as a determinant of the cellular outcome (survival versus death) following exposure to PGA2. To discern whether p21(Waf1/Cip1)-mediated protection operates through the implementation of cellular growth arrest, other growth-inhibitory treatments were studied for the ability to attenuate PGA2-induced cell death. Neither serum depletion nor suramin (a growth factor receptor antagonist) protected RKO cells against PGA2 cytotoxicity, and neither induced p21(Waf1/Cip1) expression. Mimosine, however, enhanced p21(Waf1/Cip1) expression, completely inhibited RKO cell proliferation, and exerted marked protection against a subsequent PGA2 challenge. Taken together, our results directly demonstrate a protective role for p21(Waf1/Cip1) during PGA2 cellular stress and provide strong evidence that the implementation of cellular growth arrest contributes to this protective influence. PMID:8943319

  15. Afatinib induces apoptosis in NSCLC without EGFR mutation through Elk-1-mediated suppression of CIP2A

    OpenAIRE

    Chao, Ting-Ting; Wang, Cheng-Yi; Chen, Yen-Lin; Lai, Chih-Cheng; Chang, Fang-Yu; Tsai, Yi-Ting; Chao, Chung-Hao H.; Shiau, Chung-Wai; Huang, Yuh-Chin T.; Yu, Chong-Jen; Chen, Kuen-Feng

    2014-01-01

    Afatinib has anti-tumor effect in non-small cell lung carcinoma (NSCLC) with epidermal growth factor receptor (EGFR) mutation. We found afatinib can also induce apoptosis in NSCLC cells without EGFR mutation through CIP2A pathway. Four NSCLC cell lines (H358 H441 H460 and A549) were treated with afatinib to determine their sensitivity to afatinib-induced cell death and apoptosis. The effects of CIP2A on afatinib-induced apoptosis were confirmed by overexpression and knockdown of CIP2A express...

  16. IMPACTOS SOCIALES DEL TURISMO EN EL CENTRO INTEGRALMENTE PLANEADO (CIP) BAHÍAS DE HUATULCO, MÉXICO

    OpenAIRE

    Martha Marivel Mendoza Ontiveros; Juan Carlos Monterrubio Cordero; María José Fernández Aldecua

    2011-01-01

    El Centro Integralmente Planeado (CIP) Bahías de Huatulco, México fue desarrollado con la intención de impulsar el bienestar económico y social de la región. Sin embargo, se ha propuesto que el CIP no puede ser considerado un caso del todo exitoso debido principalmente a los problemas sociales que se han presentado. En este sentido, el presente estudio tuvo como objetivo identificar la percepción de la comunidad local del CIP Huatulco sobre los beneficios y costos sociales que el turismo ha g...

  17. Sp1 regulates Raf/MEK/ERK-induced p21(CIP1) transcription in TP53-mutated cancer cells.

    Science.gov (United States)

    Karkhanis, Mansi; Park, Jong-In

    2015-03-01

    We previously reported that the upregulation of mortalin, an Hsp70 family chaperone, is important for B-Raf(V600E) tumor cells to bypass p21(CIP1) expression, which is activated as a tumor-suppressive mechanism in response to aberrant MEK/ERK activation (Wu et al., 2013). Interestingly, mortalin depletion induced p21(CIP1) transcription not only in wild-type TP53 but also in TP53-mutated B-Raf(V600E) cancer cells, suggesting the presence of an additional mechanism for p21(CIP1) regulation. In the present study, using luciferase reporter truncation analysis in a TP53-mutated B-Raf(V600E) cancer cell line, SK-MEL28, we identified a proximal p21(CIP1) promoter region responsive to mortalin depletion. Interestingly, when Sp1-like cis-elements in this promoter region were mutagenized, the p21(CIP1) promoter luciferase reporter was no longer responsive to mortalin depletion. Consistent with this, our ChIP analysis revealed that mortalin knockdown could induce Sp1 binding to p21(CIP1) promoter in a MEK/ERK-dependent manner. Moreover, RNA interference of Sp1 substantially attenuated p21(CIP1) expression induced by mortalin depletion in SK-MEL28 cells. Consistent with this observation in SK-MEL28 cells, Sp1 was necessary for the tamoxifen-regulated ∆Raf-1:ER to induce p21(CIP1) transcription in U251 cells, in which TP53 is mutated. However, in contrast, Sp1 was not necessary for ∆Raf-1:ER to induce p21(CIP1) transcription in LNCaP cells, in which TP53 is wild type. These data suggest that Sp1 may address TP53-independent p21(CIP1) transcription in Raf/MEK/ERK-activated cancer cells and that its requirement in Raf/MEK/ERK-induced p21(CIP1) transcription is subject to TP53 status.

  18. FOR Allocation to Distribution Systems based on Credible Improvement Potential (CIP)

    Science.gov (United States)

    Tiwary, Aditya; Arya, L. D.; Arya, Rajesh; Choube, S. C.

    2017-02-01

    This paper describes an algorithm for forced outage rate (FOR) allocation to each section of an electrical distribution system subject to satisfaction of reliability constraints at each load point. These constraints include threshold values of basic reliability indices, for example, failure rate, interruption duration and interruption duration per year at load points. Component improvement potential measure has been used for FOR allocation. Component with greatest magnitude of credible improvement potential (CIP) measure is selected for improving reliability performance. The approach adopted is a monovariable method where one component is selected for FOR allocation and in the next iteration another component is selected for FOR allocation based on the magnitude of CIP. The developed algorithm is implemented on sample radial distribution system.

  19. A COUPLED TH/NEUTRONICS/CRUD FRAMEWORK IN PREDICTION OF CIPS PHENOMENON

    Energy Technology Data Exchange (ETDEWEB)

    Ling Zou; Hongbin Zhang; Jess Gehin; Brendan Kochunas

    2012-04-01

    A coupled TH/Neutronics/CRUD framework, which is able to simulate the CRUD deposits impact on CIPS phenomenon, was described in this paper. This framework includes the coupling among three essential physics, thermal-hydraulics, CRUD and neutronics. The overall framework was implemented by using the CFD software STAR-CCM+, developing CRUD codes, and using the neutronics code DeCART. The coupling was implemented by exchanging data between softwares using intermediate exchange files. A typical 3 by 3 PWR fuel pin problem was solved under this framework. The problem was solved in a 12 months length period of time. Time-dependent solutions were provided, including CRUD deposits inventory and their distributions on fuels, boron hideout amount inside CRUD deposits, as well as power shape changing over time. The results clearly showed the power shape suppression in regions where CRUD deposits exist, which is a strong indication of CIPS phenomenon.

  20. The role of CIP2A protein in occurrence and development of cervical neoplasm%CIP2 A蛋白在宫颈肿瘤发生发展中的作用

    Institute of Scientific and Technical Information of China (English)

    马红梅; 徐胜美; 郭东; 周有俭; 车爱文

    2015-01-01

    目的:探讨蛋白磷酸酶2A的癌性抑制因子( CIP2A)蛋白在宫颈肿瘤发生发展中的作用。方法采用免疫组织化学SP法检测85例宫颈鳞状细胞癌、48例低级别鳞状上皮内病变、102例高级别鳞状上皮内病变及20例正常宫颈组织中CIP2A蛋白表达。并分析CIP2A的表达与临床病理参数等的关系。结果 CIP2A蛋白在正常宫颈及低级别鳞状上皮内病变组织中呈阴性表达,在高级别鳞状上皮内病变和癌组织中呈阳性表达。宫颈癌的阳性表达率明显高于高级别鳞状上皮内病变,差异有统计学意义( P 0.05)。随着宫颈癌分化程度的降低,CIP2A的阳性率逐渐升高,高分化与低分化组的差异有统计学意义( P <0.05)。CIP2A的表达与临床分期和淋巴结转移相关,差异有统计学意义( P <0.05)。结论CIP2A在宫颈高级别鳞状上皮内病变及宫颈癌组织中呈过表达,而且随着组织学分级和临床分期的进展,表达有逐渐上升的趋势;这种过表达促进癌的发生发展,并且为宫颈癌临床诊断及治疗提供新的理论依据及措施。%Objective To explore the role of CIP2A protein in occurrence and development of cervical neoplasm. Methods The expres-sion of CIP2A protein was detected in 85 cases of cervical cancer,48 cases of low squamous intraepithelial lesion,102 cases of high squamous in-traepithelial lesion and 20 cases of normal cervical tissue by immunohistochemical staining. And to analyze the relationship between CIP2A expres-sion and clinicopathological parameters and the like. Results There was negative expression of CIP2A protein observed in normal cervical tissue and low squamous intraepithelial lesion. There was positive expression of CIP2A protein observed in high squamous intraepithelial lesion and cervi-cal cancer. The positive rate in cervical cancer was significantly higher than that in high squamous intraepithelial lesion( P <0. 05

  1. An analysis of the aircraft engine Component Improvement Program (CIP) : a life cycle cost approach

    OpenAIRE

    Borer, Chris Joseph

    1990-01-01

    Approved for public release; distribution unlimited. Increasing budgetary constraints have prompted actions to reduce the maintenance cost of current naval aircraft. This thesis examines the Aircraft Engine Component Improvement Program (CIP), its impact on these cost at the organizational and intermediate levels of maintenance, and savings from these improvements. The objectives of the research were to identify current life cycle cost (LCC) models used by the Navy andor the other services...

  2. CIP2A在骨髓增生异常综合症中的意义探讨

    Institute of Scientific and Technical Information of China (English)

    王石松

    2013-01-01

    目的探讨CIP2A在骨髓增生异常综合症(MDS)中的表达及意义。方法采用免疫组化SP法对31例MDS患者、19例急性髓系白血病(AML)患者(包括4例由MDS转变而来)和20例对照组骨髓单个核细胞中CIP2A蛋白质水平进行检测。结果 MDS组CIP2A蛋白的表达水平高于对照组,差异有统计学意义(P〈0.05)。MDS高危组CIP2A蛋白的表达水平显著高于MDS低危组(P〈0.05),AML组CIP2A蛋白的表达水平显著高于MDS高危组(P〈0.05)。结论 CIP2A在MDS骨髓单个核细胞中高表达可能与MDS的疾病发展有关。

  3. Celastrol inhibits chondrosarcoma proliferation, migration and invasion through suppression CIP2A/c-MYC signaling pathway

    Directory of Open Access Journals (Sweden)

    Jinhui Wu

    2017-05-01

    Full Text Available Chondrosarcomas (CS is the second most frequent tumors of cartilage origin. A small compound extracted from Thunder God Vine (Tripterygium wilfordii Hook. F. called celastrol can directly bound CIP2A protein and effectively inhibit cell proliferation and induce apoptosis in several cancer cells. However, little knowledge is concern about the important role of CIP2A in CS patients and the therapeutic value of celastrol on CS. Our results showed that CIP2A and c-MYC were verified to be oncoproteins by detecting their mRNA and protein expression in 10 human CS tissues by qRT-PCR and Western blots. After treatment of celastrol, the proliferation, migration and invasion were significantly inhibited; whereas the apoptosis was largely induced in human CS cell lines. In addition, celastrol inhibited the expression of CIP2A, c-MYC, and suppressed apoptotic proteins BAX and caspase-8 in human CS cells, on the other hand, it induced the expression of antiapoptotic protein Bcl-2. Finally, knockdown of CIP2A also inhibited the migration and invasion and induced apoptosis of human CS cells. To sum up, we found that celastrol had effects on inhibiting proliferation, migration, invasion and inducing apoptosis through suppression CIP2A/c-MYC signaling pathway in vitro, which may provide a new therapeutic regimen for CS.

  4. Electromagnetic and absorbing property of CIPs/resin composite using the 3D forming process

    Science.gov (United States)

    Xu, Yonggang; Liang, Zichang; Wang, Xiaobing; Yuan, Liming; Li, Xinghao

    2016-08-01

    The absorbing composite filled with the flaky carbonyl iron particles (CIPs) were prepared using a three-dimensional (3D) forming process, in which the forming powder was fabricated using a milling process. The surface morphology was characterized by the scanning electron microscopy, the static magnetic property was evaluated on a vibrating sample magnetometer, and X-ray diffraction (XRD) patterns were done to analyze the particle crystal grain structure. The complex permittivity and permeability were measured using a vector network analyzer in the frequency range of 4-18 GHz. With the variable thickness was set, the reflection loss (RL) was simulated to analyze the absorbing property of the composite. The results showed that the forming powder was uniformly dispersed in the absorber, and the saturation magnetization and the grain structure of the CIPs in the forming powder nearly did not change in the milling process. With the same volume content CIPs added, the average permittivity and the imaginary permeability of the samples added the powder was smaller than the directly mixing sample due to the aggregation effect. The RL results showed that the absorbing composites using the 3D forming process with thickness 6 or 8 mm had an better absorbing property (minimum RL -13.58 and -21.85 dB) in 4-18 GHz.

  5. Interactions of ciprofloxacin (CIP), titanium dioxide (TiO{sub 2}) nanoparticles and natural organic matter (NOM) in aqueous suspensions

    Energy Technology Data Exchange (ETDEWEB)

    Fries, Elke, E-mail: elke.fries@bgr.de [Bundesanstalt für Geowissenschaften und Rohstoffe (BGR), Hannover (Germany); Bureau de Recherches Géologiques et Minières (BRGM), Orléans (France); Crouzet, Catherine; Michel, Caroline; Togola, Anne [Bureau de Recherches Géologiques et Minières (BRGM), Orléans (France)

    2016-09-01

    The aim of the present study was to investigate interactions of the antibiotic ciprofloxacin (CIP), titanium dioxide nanoparticles (TiO{sub 2} NP) and natural organic matter (NOM) in aqueous suspensions. The mean hydrodynamic diameter of particles of TiO{sub 2} NP and NOM in the suspensions ranged from 113 to 255 nm. During batch experiments the radioactivity resulting from {sup 14}CIP was determined in the filtrate (filter pore size 100 nm) by scintillation measurements. Up to 72 h, no significant sorption of NOM to TiO{sub 2} NP was observed at a TiO{sub 2} NP concentration of 5 mg/L. When the concentration of TiO{sub 2} NP was increased to 500 mg/L, a small amount of NOM of 9.5% ± 0.6% was sorbed at 72 h. The low sorption affinity of NOM on TiO{sub 2} NP surfaces could be explained by the negative charge of both components in alkaline media or by the low hydrophobicity of the NOM contents. At a TiO{sub 2} NP concentration of 5 mg L{sup −1}, the sorption of CIP on TiO{sub 2} NP was insignificant (TiO{sub 2} NP/CIP ratio: 10). When the TiO{sub 2} NP/CIP ratio was increased to 1000, a significant amount of 53.6% ± 7.2% of CIP was sorbed on TiO{sub 2} NP under equilibrium conditions at 64 h. In alkaline media, CIP is present mainly as zwitterions which have an affinity to sorb on negatively charged TiO{sub 2} NP surfaces. The sorption of CIP on TiO{sub 2} NP in the range of TiO{sub 2} NP concentrations currently estimated for municipal wastewater treatment plants is estimated to be rather low. The Freundlich sorption coefficients (K{sub F}) in the presence of NOM of 2167 L{sup n} mg mg{sup −n} kg{sup −1} was about 10 times lower than in the absence of NOM. This is an indication that the particle fraction of NOM < 100 nm could play a role as a carrier for ionic organic micro-pollutants as CIP. - Highlights: • Ciprofloxacine (CIP) and titanium dioxide nanoparticles (TiO{sub 2} NP) interact. • Organic carbon (OC) could influence such interactions. • Batch

  6. The transcriptional coactivators p/CIP and SRC-1 control insulin resistance through IRS1 in obesity models.

    Directory of Open Access Journals (Sweden)

    Zhiyong Wang

    Full Text Available Three p160 family members, p/CIP, SRC1, and TIF2, have been identified as transcriptional coactivators for nuclear hormone receptors and other transcription factors in vitro. In a previous study, we reported initial characterization of the obesity-resistant phenotypes of p/CIP and SRC-1 double knockout (DKO mice, which exhibit increased energy expenditure, and suggested that nuclear hormone receptor target genes were involved in these phenotypes. In this study, we demonstrate that p/CIP and SRC1 control insulin signaling in a cell-autonomous manner both in vitro and in vivo. Genetic deletion of p/CIP and SRC-1 increases glucose uptake and enhances insulin sensitivity in both regular chow- and high fat diet-fed DKO mice despite increased food intake. Interestingly, we discover that loss of p/CIP and SRC-1 results in resistance to age-related obesity and glucose intolerance. We show that expression levels of a key insulin signaling component, insulin receptor substrate 1 (IRS1, are significantly increased in two cell lines representing fat and muscle lineages with p/CIP and SRC-1 deletions and in white adipose tissue and skeletal muscle of DKO mice; this may account for increased glucose metabolism and insulin sensitivity. This is the first evidence that the p160 coactivators control insulin signaling and glucose metabolism through IRS1. Therefore, our studies indicate that p/CIP and SRC-1 are potential therapeutic targets not only for obesity but also for diabetes.

  7. EBV-positive Hodgkin lymphoma is associated with suppression of p21cip1/waf1 and a worse prognosis

    Directory of Open Access Journals (Sweden)

    Hsu Su-Ming

    2010-02-01

    Full Text Available Abstract Background About 30-50% of Hodgkin lymphomas (HLs harbor the Epstein-Barr virus (EBV, but the impact of EBV infection on clinical outcomes has been unclear. EBV-encoded small RNAs (EBERs are presented in all EBV-infected cells, but their functions are still less understood. Results EBER1 was transfected into two HL cell lines, KMH2 and L428, and microarrays were used to screen for EBER1-induced changes. We found that EBER1 suppressed p21cip1/waf1 transcription in HL cell lines. In addition, positive regulators of p21cip1/waf1 transcription, such as p53, EGR1, and STAT1, were decreased. Suppression of p21cip1/waf1 in the EBER1+ HL cell lines was associated with increased resistance to histone deacetylase inhibitors or proteasome inhibitors, drugs known to cause apoptosis by increasing p21cip1/waf1 levels. On biopsy specimens, EBV+ HLs had weaker expression of both p21cip1/waf1 and active caspase 3. Clinically, suppression of p21cip1/waf1 in EBV+ HLs was associated with a worse 2-year disease-free survival rate (45% for EBV+ HLs vs. 77% for EBV- HLs, p = 0.002. Conclusion Although the underlying mechanisms are still relatively unclear, EBER1 inhibits p21cip1/waf1 transcription and prevents apoptosis through down-regulation of p53, EGR1, and STAT1. The anti-apoptotic activity of EBER1 may be important in the rescue of Reed-Sternberg cells from drug-induced apoptosis and in the clinical behaviors of EBV+ HLs.

  8. Persistent longitudinal variations in 8 years of CIPS/AIM polar mesospheric clouds

    Science.gov (United States)

    Liu, Xiao; Yue, Jia; Xu, Jiyao; Yuan, Wei; Russell, James M.; Hervig, M. E.; Nakamura, Takuji

    2016-07-01

    The Cloud Imaging and Particle Size (CIPS) instrument on the Aeronomy of Ice in the Mesosphere (AIM) satellite provides an opportunity to study the longitudinal variation in polar mesospheric cloud (PMC). We examined the longitudinal variation in PMC albedo using 8 years (2007-2014) of observations from the CIPS instrument. The results show that the PMC albedo in the Southern Hemisphere (SH), especially in the latitude band of 80°S-85°S, is persistently low ( 65% relative to the rest of the hemisphere) within 60°W to 150°W longitude. In the Northern Hemisphere (NH), however, PMC albedo is found to be relatively zonally asymmetry. Harmonic analyses show that the persistent longitudinal variation in the SH PMC albedo is due to zonal wave numbers 1 through 4 (WN1-WN4) processes with minima in the longitude range of 60°W-150°W. The influence of temperature and H2O on the longitudinal variation of the PMC albedo is discussed based on results obtained using a simple 0-D PMC model and temperature from the Microwave Limb Sounder (MLS) and the Sounding of the Atmosphere with Broadband Emission Radiometry (SABER) and H2O from MLS. The modeled region of low ice mass in the SH is generally consistent with that of low PMC albedo seen in CIPS. Tidal analyses using the SABER temperatures indicate that the nonmigrating semidiurnal tides with modes of S0, W1, and E1 might be the main drivers of the persistent longitudinal variations of PMC albedo in the SH. Nonmigrating tides are much weaker in the NH and consistent with the observed lack of longitudinal variability in PMC albedo.

  9. Inline UV-Vis spectroscopy to monitor and optimize cleaning-in-place (CIP) of whey filtration plants

    DEFF Research Database (Denmark)

    Berg, Thilo Heinz Alexander; Ottosen, Niels; van der Berg, Franciscus Winfried J.

    2017-01-01

    used for every day. We investigated the capability of inline UV-Vis spectroscopy to elucidate the dynamics of CIP of membrane filtration plants as a gateway to control and optimize the process. For this investigation aged membranes that had been used for industrial ultrafiltration of whey were...... transferred to a pilot plant equipped with inline UV-Vis spectroscopy on both the retentate and permeate side. Then the dynamics of multiple fouling and cleaning of these membranes were investigated. The results indicate that the first CIP step, caustic cleaning could be shortened and possibly reduced...... in concentration. The second step of CIP, enzymatic cleaning, seems to be active even longer than the anticipated time. Challenges, first findings and future steps of full scale inline UV-Vis spectroscopy are discussed. We conclude that inline UV-Vis spectroscopy can be used to optimize the processing time, energy...

  10. THE EXPRESSION AND SIGNIFICANCE OF P53 AND P21(WAF1/CIP1) IN THYROID CARCINOMA

    Institute of Scientific and Technical Information of China (English)

    Huo Xiongwei; Ma Qingyong; Gao Yanfeng; Sun Xuejun; Liu Hao; Sheng Wei

    2005-01-01

    Objective To determine the expression of P53 and P21 (WAF1/CIP1) in thyroid carcinomas and its relationship with development and prognosis of the carcinoma. Methods 90 cases of thyroid tissues (60 thyroid carcinomas, 10 thyroid adenomas, 10 goitres and 10 normal thyroid tissues) were studied by SP immunohistochemical method. Results Positive immunoreactivity of P53 and P21(WAF1/CIP1) was found only in thyroid carcinomas. The positive rate of the P53 and P21 is 53.3% and 41.7% respectively. The positive-staining rates of P53 were higher in cases of undifferentiated carcinomas, positive metastasis lymph nodes or in stage Ⅲ, Ⅳ than those in the cases of well-differentiated, no metastasis lymph nodes, or in stage Ⅰ,Ⅱ. In addition, the positive-staining of P21(WAF1/CIP1) were lower in cases of undifferentiated carcinomas, positive metastasis lymph nodes or stage Ⅲ, Ⅳ than that in the cases of well-differentiated, no metastasis lymph nodes or in stage Ⅰ,Ⅱ. The P21 (WAF1/CIP1) expression rate in the P53 positive group was lower than that in the P53 negative group (P<0.05). Conclusion The expression of P21(WAF1/CIP1) protein in thyroid cancer is related to P53-depend pathway and P53-independent pathway, mainly the P53-depend pathway. Examination of expression of P53 and P21 (WAF1/ CIP1) proteins may be helpful to judge the thyroid cancers behavior and prognosis.

  11. Effect of the bio-absorbent on the microwave absorption property of the flaky CIPs/rubber absorbers

    Energy Technology Data Exchange (ETDEWEB)

    Cheng, Yang; Xu, Yonggang, E-mail: xuyonggang221@163.com; Cai, Jun; Yuan, Liming; Zhang, Deyuan

    2015-09-01

    Microwave absorbing composites filled with flaky carbonyl iron particles (CIPs) and the bio-absorbent were prepared by using a two-roll mixer and a vulcanizing machine. The electromagnetic (EM) parameters were measured by a vector network analyzer and the reflection loss (RL) was measured by the arch method in the frequency range of 1–4 GHz. The uniform dispersion of the absorbents was verified by comparing the calculated RL with the measured one. The results confirm that as the bio-absorbent was added, the permittivity was increased due to the volume content of absorbents, and the permeability was enlarged owing to the volume content of CIPs and interactions between the two absorbents. The composite filled with bio-absorbents achieved an excellent absorption property at a thickness of 1 mm (minimum RL reaches −7.8 dB), and as the RL was less than −10 dB the absorption band was widest (2.1–3.8 GHz) at a thickness of 2 mm. Therefore, the bio-absorbent is a promising additive candidate on fabricating microwave absorbing composites with a thinner thickness and wider absorption band. - Graphical abstract: Morphology of composites filled with flaky CIPs and the bio-absorbent. The enhancement of bio-absorbent on the electromagnetic absorption property of composites filled with flaky carbonyl iron particles (CIPs) is attributed to the interaction of the two absorbents. The volume content of the FCMPs with the larger shape CIPs play an important role in this effects, the composites filled with irons and bio-absorbents can achieve wider-band and thinner-thickness absorbing materials. - Highlights: • Absorbers filled with bio-absorbents and CIPs was fabricated. • Bio-absorbents enhanced the permittivity and permeability of the composites. • The absorbent interactions play a key role in the enhancement mechanism. • Bio-absorbents enhanced the composite RL in 1–4 GHz.

  12. The calcium channel blocker amlodipine exerts its anti-proliferative action via p21(Waf1/Cip1) gene activation.

    Science.gov (United States)

    Ziesche, Rolf; Petkov, Ventzislav; Lambers, Christopher; Erne, Paul; Block, Lutz-Henning

    2004-10-01

    Proliferation of vascular smooth muscle cells (VSMC) contributes to the progression of atherosclerotic plaques. Calcium channel blockers have been shown to reduce VSMC proliferation, but the underlying molecular mechanism remains unclear. p21(Waf1/Cip1) is a potent inhibitor of cell cycle progression. Here, we demonstrate that amlodipine (10(-6) to 10(-8) M) activates de novo synthesis of p21(Waf1/Cip1) in vitro. We show that amlodipine-dependent activation of p21(Waf1/Cip1) involves the action of the glucocorticoid receptor (GR) and C/EBP-alpha. The underlying pathway apparently involves the action of mitogen-activated protein kinase or protein kinase C, but not of extracellular signal-related kinase or changes of intracellular calcium. Amlodipine-induced p21(Waf1/Cip1) promoter activity and expression were abrogated by C/EBP-alpha antisense oligonucleotide or by the GR antagonist RU486. Amlodipine-dependent inhibition of cell proliferation was partially reversed by RU486 at 10(-8) M (58%+/-29%), antisense oligonucleotides targeting C/EBP-alpha (91%+/-26%), or antisense mRNAs targeting p21(Waf1/Cip1) (96%+/-32%, n=6); scrambled antisense oligonucleotides or those directed against C/EBP-beta were ineffective. The data suggest that the anti-proliferative action of amlodipine is achieved by induction of the p21 (Waf1/Cip1) gene, which may explain beneficial covert effects of this widely used cardiovascular therapeutic drug beyond a more limited role as a vascular relaxant.

  13. Cip/Kip cyclin-dependent protein kinase inhibitors and the road to polyploidy

    Directory of Open Access Journals (Sweden)

    DePamphilis Melvin L

    2009-06-01

    Full Text Available Abstract Cyclin-dependent kinases (CDKs play a central role in the orderly transition from one phase of the eukaryotic mitotic cell division cycle to the next. In this context, p27Kip1 (one of the CIP/KIP family of CDK specific inhibitors in mammals or its functional analogue in other eukarya prevents a premature transition from G1 to S-phase. Recent studies have revealed that expression of a second member of this family, p57Kip2, is induced as trophoblast stem (TS cells differentiate into trophoblast giant (TG cells. p57 then inhibits CDK1 activity, an enzyme essential for initiating mitosis, thereby triggering genome endoreduplication (multiple S-phases without an intervening mitosis. Expression of p21Cip1, the third member of this family, is also induced in during differentiation of TS cells into TG cells where it appears to play a role in suppressing the DNA damage response pathway. Given the fact that p21 and p57 are unique to mammals, the question arises as to whether one or both of these proteins are responsible for the induction and maintenance of polyploidy during mammalian development.

  14. A CIP-Based Numerical Simulation of Free Surface Flow Related to Freak Waves

    Institute of Scientific and Technical Information of China (English)

    赵西增

    2013-01-01

    An enhanced numerical model for simulating two-dimensional incompressible viscous flow with distorted free surface is reported. The numerical simulation is carried out through the CIP (Constrained Interpolation Profile)-based method, which is described in the paper. A more accurate interface capturing scheme, the VOF/WLIC scheme (VOF:Volume-of-Fluid;WLIC:weighed line interface calculation), is adopted as the interface capturing method. To assess the developed algorithm and its versatility, a selection of test problems are examined, i.e. the square wave propagation, the Zalesak’s rigid body rotation, dam breaking problem with and without obstacles, wave sloshing in an excited wave tank and interaction between extreme waves and a floating body. Excellent agreements are obtained when numerical results are compared with available analytical, experimental, and other numerical results. These examples demonstrate that the use of the VOF/WLIC scheme in the free surface capturing makes better results and also the proposed CIP-based model is capable of predicting the freak wave-related phenomena.

  15. Sensitivity of CIPS-computed PVC location to measurement errors in ECG electrode position: the need for the 3D Camera

    NARCIS (Netherlands)

    Dam, P.M. van; Gordon, J.P.; Laks, M.

    2014-01-01

    BACKGROUND: The Cardiac Isochrone Positioning System (CIPS) is a non-invasive method able to localize the origins of PVCs, VT and WPW from the 12 lead ECG. The CIPS model integrates a standard 12-lead ECG with an MRI derived model of the heart, lungs, and torso in order to compute the precise electr

  16. Label-free quantitative phosphoproteomics with novel pairwise abundance normalization reveals synergistic RAS and CIP2A signaling.

    Science.gov (United States)

    Kauko, Otto; Laajala, Teemu Daniel; Jumppanen, Mikael; Hintsanen, Petteri; Suni, Veronika; Haapaniemi, Pekka; Corthals, Garry; Aittokallio, Tero; Westermarck, Jukka; Imanishi, Susumu Y

    2015-08-17

    Hyperactivated RAS drives progression of many human malignancies. However, oncogenic activity of RAS is dependent on simultaneous inactivation of protein phosphatase 2A (PP2A) activity. Although PP2A is known to regulate some of the RAS effector pathways, it has not been systematically assessed how these proteins functionally interact. Here we have analyzed phosphoproteomes regulated by either RAS or PP2A, by phosphopeptide enrichment followed by mass-spectrometry-based label-free quantification. To allow data normalization in situations where depletion of RAS or PP2A inhibitor CIP2A causes a large uni-directional change in the phosphopeptide abundance, we developed a novel normalization strategy, named pairwise normalization. This normalization is based on adjusting phosphopeptide abundances measured before and after the enrichment. The superior performance of the pairwise normalization was verified by various independent methods. Additionally, we demonstrate how the selected normalization method influences the downstream analyses and interpretation of pathway activities. Consequently, bioinformatics analysis of RAS and CIP2A regulated phosphoproteomes revealed a significant overlap in their functional pathways. This is most likely biologically meaningful as we observed a synergistic survival effect between CIP2A and RAS expression as well as KRAS activating mutations in TCGA pan-cancer data set, and synergistic relationship between CIP2A and KRAS depletion in colony growth assays.

  17. Development of the radical-stable Coprinus cinereus peroxidase (CiP) by blocking the radical attack.

    Science.gov (United States)

    Kim, Su Jin; Joo, Jeong Chan; Kim, Han Sang; Kwon, Inchan; Song, Bong Keun; Yoo, Young Je; Kim, Yong Hwan

    2014-11-10

    Despite the potential use of peroxidases as industrial biocatalysts, their practical application is often impeded due to suicide inactivation by radicals generated in oxidative reactions. Using a peroxidase from Coprinus cinereus (CiP) as a model enzyme, we revealed a dominant factor for peroxidase inactivation during phenol oxidation, and we engineered radical-stable mutants by site-directed mutagenesis of an amino acid residue susceptible to modification by phenoxyl radical. Mass spectrometry analysis of inactivated CiP identified an adduct between F230 and a phenoxyl radical, and subsequently, the F230 residue was mutated to amino acids that resisted radical coupling. Of the F230 mutants, the F230A mutant showed the highest stability against radical inactivation, retaining 80% of its initial activity, while the wild-type protein was almost completely inactivated. The F230A mutant also exhibited a 16-fold higher turnover of the phenol substrate compared with the wild-type enzyme. Furthermore, the F230A mutant was stable during the oxidation of other phenolic compounds, including m-cresol and 3-methoxyphenol. No structural changes were observed by UV-vis and CD spectra of CiP after radical coupling, implying that the F230-phenol radical adduct inactivated CiP by blocking substrate access to the active site. Our novel strategy can be used to improve the stability of other peroxidases inactivated by radicals.

  18. Serine 62-Phosphorylated MYC Associates with Nuclear Lamins and Its Regulation by CIP2A Is Essential for Regenerative Proliferation.

    Science.gov (United States)

    Myant, Kevin; Qiao, Xi; Halonen, Tuuli; Come, Christophe; Laine, Anni; Janghorban, Mahnaz; Partanen, Johanna I; Cassidy, John; Ogg, Erinn-Lee; Cammareri, Patrizia; Laiterä, Tiina; Okkeri, Juha; Klefström, Juha; Sears, Rosalie C; Sansom, Owen J; Westermarck, Jukka

    2015-08-11

    An understanding of the mechanisms determining MYC's transcriptional and proliferation-promoting activities in vivo could facilitate approaches for MYC targeting. However, post-translational mechanisms that control MYC function in vivo are poorly understood. Here, we demonstrate that MYC phosphorylation at serine 62 enhances MYC accumulation on Lamin A/C-associated nuclear structures and that the protein phosphatase 2A (PP2A) inhibitor protein CIP2A is required for this process. CIP2A is also critical for serum-induced MYC phosphorylation and for MYC-elicited proliferation induction in vitro. Complementary transgenic approaches and an intestinal regeneration model further demonstrated the in vivo importance of CIP2A and serine 62 phosphorylation for MYC activity upon DNA damage. However, targeting of CIP2A did not influence the normal function of intestinal crypt cells. These data underline the importance of nuclear organization in the regulation of MYC phosphorylation, leading to an in vivo demonstration of a strategy for inhibiting MYC activity without detrimental physiological effects.

  19. Serine 62-Phosphorylated MYC Associates with Nuclear Lamins and Its Regulation by CIP2A Is Essential for Regenerative Proliferation

    Directory of Open Access Journals (Sweden)

    Kevin Myant

    2015-08-01

    Full Text Available An understanding of the mechanisms determining MYC’s transcriptional and proliferation-promoting activities in vivo could facilitate approaches for MYC targeting. However, post-translational mechanisms that control MYC function in vivo are poorly understood. Here, we demonstrate that MYC phosphorylation at serine 62 enhances MYC accumulation on Lamin A/C-associated nuclear structures and that the protein phosphatase 2A (PP2A inhibitor protein CIP2A is required for this process. CIP2A is also critical for serum-induced MYC phosphorylation and for MYC-elicited proliferation induction in vitro. Complementary transgenic approaches and an intestinal regeneration model further demonstrated the in vivo importance of CIP2A and serine 62 phosphorylation for MYC activity upon DNA damage. However, targeting of CIP2A did not influence the normal function of intestinal crypt cells. These data underline the importance of nuclear organization in the regulation of MYC phosphorylation, leading to an in vivo demonstration of a strategy for inhibiting MYC activity without detrimental physiological effects.

  20. Constitutive cytoplasmic localization of p21(Waf1/Cip1) affects the apoptotic process in monocytic leukaemia

    NARCIS (Netherlands)

    Schepers, H; Geugien, M; Eggen, BJL; Vellenga, E

    2003-01-01

    In the present study, we analysed the expression and localization of p21(Waf1/Cip1) in normal and malignant haematopoietic cells. We demonstrate that in normal monocytic cells, protein kinase C (PKC)-induced p21 gene activation, which is nuclear factor-kappaB (NF-kappaB) independent, results in pred

  1. ETS1 mediates MEK1/2-dependent overexpression of cancerous inhibitor of protein phosphatase 2A (CIP2A in human cancer cells.

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    Anchit Khanna

    Full Text Available EGFR-MEK-ERK signaling pathway has an established role in promoting malignant growth and disease progression in human cancers. Therefore identification of transcriptional targets mediating the oncogenic effects of the EGFR-MEK-ERK pathway would be highly relevant. Cancerous inhibitor of protein phosphatase 2A (CIP2A is a recently characterized human oncoprotein. CIP2A promotes malignant cell growth and is over expressed at high frequency (40-80% in most of the human cancer types. However, the mechanisms inducing its expression in cancer still remain largely unexplored. Here we present systematic analysis of contribution of potential gene regulatory mechanisms for high CIP2A expression in cancer. Our data shows that evolutionary conserved CpG islands at the proximal CIP2A promoter are not methylated both in normal and cancer cells. Furthermore, sequencing of the active CIP2A promoter region from altogether seven normal and malignant cell types did not reveal any sequence alterations that would increase CIP2A expression specifically in cancer cells. However, treatment of cancer cells with various signaling pathway inhibitors revealed that CIP2A mRNA expression was sensitive to inhibition of EGFR activity as well as inhibition or activation of MEK-ERK pathway. Moreover, MEK1/2-specific siRNAs decreased CIP2A protein expression. Series of CIP2A promoter-luciferase constructs were created to identify proximal -27 to -107 promoter region responsible for MEK-dependent stimulation of CIP2A expression. Additional mutagenesis and chromatin immunoprecipitation experiments revealed ETS1 as the transcription factor mediating stimulation of CIP2A expression through EGFR-MEK pathway. Thus, ETS1 is probably mediating high CIP2A expression in human cancers with increased EGFR-MEK1/2-ERK pathway activity. These results also suggest that in addition to its established role in invasion and angiogenesis, ETS1 may support malignant cellular growth via regulation of

  2. The development of a thermostable CiP (Coprinus cinereus peroxidase) through in silico design.

    Science.gov (United States)

    Kim, Su Jin; Lee, Jeong Ah; Joo, Jeong Chan; Yoo, Young Je; Kim, Yong Hwan; Song, Bong Keun

    2010-01-01

    Protein thermostability is a crucial issue in the practical application of enzymes, such as inorganic synthesis and enzymatic polymerization of phenol derivatives. Much attention has been focused on the enhancement and numerous successes have been achieved through protein engineering methods. Despite fruitful results based on random mutagenesis, it was still necessary to develop a novel strategy that can reduce the time and effort involved in this process. In this study, a rapid and effective strategy is described for increasing the thermal stability of a protein. Instead of random mutagenesis, a rational strategy was adopted to theoretically stabilize the thermo labile residues of a protein using computational methods. Protein residues with high flexibility can be thermo labile due to their large range of movement. Here, residue B factor values were used to identify putatively thermo labile residues and the RosettaDesign program was applied to search for stable sequences. Coprinus cinereus (CiP) heme peroxidase was selected as a model protein for its importance in commercial applications, such as the polymerization of phenolic compounds. Eleven CiP residues with the highest B factor values were chosen as target mutation sites for thermostabilization, and then redesigned using RosettaDesign to identify sequences. Eight mutants based on the redesigns, were produced as functional enzymes and two of these (S323Y and E328D) showed increased thermal stability over the wild-type in addition to conserved catalytic activity. Thus, this strategy can be used as a rapid and effective in silico design tool for obtaining thermostable proteins.

  3. Centrosomal AKAP350 and CIP4 act in concert to define the polarized localization of the centrosome and Golgi in migratory cells

    Science.gov (United States)

    Tonucci, Facundo M.; Hidalgo, Florencia; Ferretti, Anabela; Almada, Evangelina; Favre, Cristián; Goldenring, James R.; Kaverina, Irina; Kierbel, Arlinet; Larocca, M. Cecilia

    2015-01-01

    ABSTRACT The acquisition of a migratory phenotype is central in processes as diverse as embryo differentiation and tumor metastasis. An early event in this phenomenon is the generation of a nucleus–centrosome–Golgi back-to-front axis. AKAP350 (also known as AKAP9) is a Golgi and centrosome scaffold protein that is involved in microtubule nucleation. AKAP350 interacts with CIP4 (also known as TRIP10), a cdc42 effector that regulates actin dynamics. The present study aimed to characterize the participation of centrosomal AKAP350 in the acquisition of migratory polarity, and the involvement of CIP4 in the pathway. The decrease in total or in centrosomal AKAP350 led to decreased formation of the nucleus–centrosome–Golgi axis and defective cell migration. CIP4 localized at the centrosome, which was enhanced in migratory cells, but inhibited in cells with decreased centrosomal AKAP350. A decrease in the CIP4 expression or inhibition of the CIP4–AKAP350 interaction also led to defective cell polarization. Centrosome positioning, but not nuclear movement, was affected by loss of CIP4 or AKAP350 function. Our results support a model in which AKAP350 recruits CIP4 to the centrosome, providing a centrosomal scaffold to integrate microtubule and actin dynamics, thus enabling centrosome polarization and ensuring cell migration directionality. PMID:26208639

  4. Regulation of P21cip1 Expression by Growth Factors and the Extracellular Matrix Reveals a Role for Transient ERK Activity in G1 Phase

    Science.gov (United States)

    Bottazzi, Maria Elena; Zhu, Xiaoyun; Böhmer, Ralph M.; Assoian, Richard K.

    1999-01-01

    We have examined the regulation of p21cip1 by soluble mitogens and cell anchorage as well as the relationship between the expression of p21cip1 and activation of the ERK subfamily of MAP kinases. We find that p21cip1 expression in G1 phase can be divided into two discrete phases: an initial induction that requires growth factors and the activation of ERK, and then a subsequent decline that is enhanced by cell anchorage in an ERK-independent manner. In contrast to the induction of cyclin D1, the induction of p21cip1 is mediated by transient ERK activity. Comparative studies with wild-type and p21cip1-null fibroblasts indicate that adhesion-dependent regulation of p21cip1 is important for proper control of cyclin E–cdk2 activity. These data lead to a model in which mitogens and anchorage act in a parallel fashion to regulate G1 phase expression of p21cip1. They also show that (a) growth factors and growth factor/extracellular matrix cooperation can have different roles in regulating G1 phase ERK activity and (b) both transient and sustained ERK signals have functionally significant roles in controlling cell cycle progression through G1 phase. PMID:10491389

  5. CIP2A modulates cell-cycle progression in human cancer cells by regulating the stability and activity of Plk1.

    Science.gov (United States)

    Kim, Jae-Sung; Kim, Eun Ju; Oh, Jeong Su; Park, In-Chul; Hwang, Sang-Gu

    2013-11-15

    Abnormal cell-cycle control can lead to aberrant cell proliferation and cancer. The oncoprotein cancerous inhibitor of protein phosphatase 2A (CIP2A) is an inhibitor of protein phosphatase 2A (PP2A) that stabilizes c-Myc. However, the precise role of CIP2A in cell division is not understood. Herein, we show that CIP2A is required for mitotic progression by regulating the polo-like kinase (Plk1). With mitotic entry, CIP2A translocated from the cytoplasm to the nucleus, where it was enriched at spindle poles. CIP2A depletion delayed mitotic progression, resulting in mitotic abnormalities independent of PP2A activity. Unexpectedly, CIP2A interacted directly with the polo-box domain of Plk1 during mitosis. This interaction was required to maintain Plk1 stability by blocking APC/C-Cdh1-dependent proteolysis, thereby enhancing the kinase activity of Plk1 during mitosis. We observed strong correlation and in vivo interactions between these two proteins in multiple human cancer specimens. Overall, our results established a novel function for CIP2A in facilitating the stability and activity of the pivotal mitotic kinase Plk1 in cell-cycle progression and tumor development.

  6. TGFbeta induces apoptosis and EMT in primary mouse hepatocytes independently of p53, p21Cip1 or Rb status

    Directory of Open Access Journals (Sweden)

    Harrison David J

    2008-07-01

    Full Text Available Abstract Background TGFβ has pleiotropic effects that range from regulation of proliferation and apoptosis to morphological changes and epithelial-mesenchymal transition (EMT. Some evidence suggests that these effects may be interconnected. We have recently reported that P53, P21Cip1 and pRB, three critical regulators of the G1/S transition are variably involved in TGFβ-induced cell cycle arrest in hepatocytes. As these proteins are also involved in the regulation of apoptosis in many circumstances, we investigated their contribution to other relevant TGFβ-induced effects, namely apoptosis and EMT, and examined how the various processes were interrelated. Methods Primary mouse hepatocytes deficient in p53, p21 and/or Rb, singly or in combination were treated with TGFβ for 24 to 96 hours. Apoptosis was quantified according to morphology and by immunostaining for cleaved-capsase 3. Epithelial and mesenchymal marker expression was studied using immunocytochemistry and real time PCR. Results We found that TGFβ similarly induced morphological changes regardless of genotype and independently of proliferation index or sensitivity to inhibition of proliferation by TGFβ. Morphological changes were accompanied by decrease in E-cadherin and increased Snail expression but the mesenchymal markers (N-cadherin, SMAα and Vimentin studied remained unchanged. TGFβ induced high levels of apoptosis in p53-/-, Rb-/-, p21cip1-/- and control hepatocytes although with slight differences in kinetics. This was unrelated to proliferation or changes in morphology and loss of cell-cell adhesion. However, hepatocytes deficient in both p53 and p21cip1were less sensitive to TGFβ-induced apoptosis. Conclusion Although p53, p21Cip1 and pRb are well known regulators of both proliferation and apoptosis in response to a multitude of stresses, we conclude that they are critical for TGFβ-driven inhibition of hepatocytes proliferation, but only slightly modulate TGF

  7. CIPS Risk Analysis for AP1000 PWR First Cycle%AP1000核电厂首循环CIPS风险评价

    Institute of Scientific and Technical Information of China (English)

    杨萍; 汤春桃

    2012-01-01

    CIPS(Crud Induced Power Shift),是指反应堆冷却剂系统内腐蚀积垢物在燃料棒表面沉积而导致轴向功率峰值向堆芯入口处偏移的现象.这一现象会影响核电厂运行的安全性,如:使降功率时轴向功率分布控制困难、停堆裕量减少、反应堆启堆时的临界工况评价出现偏差等,进而影响经济性.本文阐述了CIPS现象的成因、危害、风险降低途径,并使用3D核设计程序、热工-水力子通道程序和CIPS评价专用程序对AP1000核电厂首循环的CIPS风险进行了分析并给出了降低风险的建议.%CIPS (Crud Induced Power Shift) is a shift in axial power towards the core inlet which results from the build up of crud on the fuel rods during operation. The impact of CIPS is that axial power distribution control is more difficult. The shutdown margin is also reduced after a trip. The increased reactivity difference between the HFP and HZP can also cause a misprediction of Estimated Critical Condition on the restart after trip or shutdown. In the report, the cause and impacts of CIPS and the ways to reduce the CIPS risk have been described. Additionally,3D nuclear design code,thermal-hydraulic subchannel code and special CIPS analysis code have been combined together to analysis CIPS risk for AP1000 first cycle. At last,some advices on reducing CIPS risk have been presented.

  8. Final Environmental Assessment for Capital Improvements Program (CIP) at Davis-Monthan Air Force Base Tucson, Arizona

    Science.gov (United States)

    2008-06-01

    controlled drones or sold to allied forces. FINAL ENVIRONMENTAL ASSESSMENT Capital Improvements Program (CIP) at Davis-Monthan AFB 1-3 10 19 Tuc s on...accident. APZs follow arrival, departure, and pattern flight tracks and are based upon analysis of historical data. The CZs at Davis-Monthan AFB are...the region. Cattle ranching began after 1865, with American ranchers establishing extensive operations during the 1880s. Most settlement occurred

  9. Photodynamic therapy results in induction of WAF1/CIP1/P21 leading to cell cycle arrest and apoptosis.

    Science.gov (United States)

    Ahmad, N; Feyes, D K; Agarwal, R; Mukhtar, H

    1998-06-09

    Photodynamic therapy (PDT) is a promising new modality that utilizes a combination of a photosensitizing chemical and visible light for the management of a variety of solid malignancies. The mechanism of PDT-mediated cell killing is not well defined. We investigated the involvement of cell cycle regulatory events during silicon phthalocyanine (Pc4)-PDT-mediated apoptosis in human epidermoid carcinoma cells A431. PDT resulted in apoptosis, inhibition of cell growth, and G0-G1 phase arrest of the cell cycle, in a time-dependent fashion. Western blot analysis revealed that PDT results in an induction of the cyclin kinase inhibitor WAF1/CIP1/p21, and a down-regulation of cyclin D1 and cyclin E, and their catalytic subunits cyclin-dependent kinase (cdk) 2 and cdk6. The treatment also resulted in a decrease in kinase activities associated with all the cdks and cyclins examined. PDT also resulted in (i) an increase in the binding of cyclin D1 and cdk6 toward WAF1/CIP1/p21, and (ii) a decrease in the binding of cyclin D1 toward cdk2 and cdk6. The binding of cyclin E and cdk2 toward WAF1/CIP1/p21, and of cyclin E toward cdk2 did not change by the treatment. These data suggest that PDT-mediated induction of WAF1/CIP1/p21 results in an imposition of artificial checkpoint at G1 --> S transition thereby resulting in an arrest of cells in G0-G1 phase of the cell cycle through inhibition in the cdk2, cdk6, cyclin D1, and cyclin E. We suggest that this arrest is an irreversible process and the cells, unable to repair the damages, ultimately undergo apoptosis.

  10. p21WAF1/Cip1 expression is associated with cell differentiation but not with p53 mutations in squamous cell carcinomas of the larynx.

    Science.gov (United States)

    Nadal, A; Jares, P; Cazorla, M; Fernández, P L; Sanjuan, X; Hernandez, L; Pinyol, M; Aldea, M; Mallofré, C; Muntané, J; Traserra, J; Campo, E; Cardesa, A

    1997-10-01

    p21WAF1/Cip1 is a recently identified gene involved in cell cycle regulation through cyclin-CDK-complex inhibition. The expression of this gene in several cell lines seems to be induced by wild-type, but not mutant, p53. p21WAF1/Cip1 expression has been studied at both mRNA and protein levels in a series of 49 normal mucosae and squamous cell carcinomas of the larynx. A significant association was found between mRNA and protein expression in tumours (P global S-phase of the carcinomas. p53 mutations (exons 5-9) were found in ten carcinomas with p21WAF1/Cip1 expression, but no p53 mutations were detected in three p21WAF1/Cip1-negative tumours. In conclusion, p21WAF1/Cip1 expression is frequently upregulated in squamous cell carcinomas of the larynx and is associated with tumour cell differentiation. p21WAF1/Cip1 expression in these tumours is independent of p53 gene mutations.

  11. pH induced polychromatic UV treatment for the removal of a mixture of SMX, OTC and CIP from water

    Energy Technology Data Exchange (ETDEWEB)

    Avisar, D., E-mail: droravi@post.tau.ac.il [Hydro-chemistry Laboratory, Geography and the Environment, Tel Aviv University, Tel Aviv 69978 (Israel); Lester, Y. [Hydro-chemistry Laboratory, Geography and the Environment, Tel Aviv University, Tel Aviv 69978 (Israel); School of Mechanical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv 69978 (Israel); Mamane, H. [School of Mechanical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv 69978 (Israel)

    2010-03-15

    Water and wastewater effluents contain a vast range of chemicals in mixtures that have different chemical structures and characteristics. This study presents a treatment technology for the removal of mixtures of antibiotic residues (sulfamethoxazole (SMX), oxtetracycline (OTC) and ciprofloxacin (CIP)) from contaminated water. The treatment combines pH modification of the water to an optimal value, followed by a photolytic treatment using direct polychromatic ultraviolet (UV) irradiation by medium pressure UV lamp. The pH adjustment of the treated water leads to structural modifications of the pollutant's molecule thus may enhance direct photolysis by UV light. Results showed that an increase of water pH from 5 to 7 leads to a decrease in degradation rate of SMX and an increase in degradation rate of OTC and CIP, when studied separately and not in a mixture. Thus, the optimal pH values for UV photodegradation in a mixture, involve initial photolysis at pH 5 and then gradually changing the pH from 5 to 7 during the UV exposure. For example, this resulted in 99% degradation of SMX at pH 5 and enhanced degradation of OTC and CIP from 54% and 26% to 91% and 96% respectively when pH was increased from 5 to 7. Thus the pH induced photolytic treatment has a potential in improving treatment of antibiotics in mixtures.

  12. pH induced polychromatic UV treatment for the removal of a mixture of SMX, OTC and CIP from water.

    Science.gov (United States)

    Avisar, D; Lester, Y; Mamane, H

    2010-03-15

    Water and wastewater effluents contain a vast range of chemicals in mixtures that have different chemical structures and characteristics. This study presents a treatment technology for the removal of mixtures of antibiotic residues (sulfamethoxazole (SMX), oxytetracycline (OTC) and ciprofloxacin (CIP)) from contaminated water. The treatment combines pH modification of the water to an optimal value, followed by a photolytic treatment using direct polychromatic ultraviolet (UV) irradiation by medium pressure UV lamp. The pH adjustment of the treated water leads to structural modifications of the pollutant's molecule thus may enhance direct photolysis by UV light. Results showed that an increase of water pH from 5 to 7 leads to a decrease in degradation rate of SMX and an increase in degradation rate of OTC and CIP, when studied separately and not in a mixture. Thus, the optimal pH values for UV photodegradation in a mixture, involve initial photolysis at pH 5 and then gradually changing the pH from 5 to 7 during the UV exposure. For example, this resulted in 99% degradation of SMX at pH 5 and enhanced degradation of OTC and CIP from 54% and 26% to 91% and 96% respectively when pH was increased from 5 to 7. Thus the pH induced photolytic treatment has a potential in improving treatment of antibiotics in mixtures. (c) 2009 Elsevier B.V. All rights reserved.

  13. Targeting p35/Cdk5 Signalling via CIP-Peptide Promotes Angiogenesis in Hypoxia

    Science.gov (United States)

    Bosutti, Alessandra; Qi, Jie; Pennucci, Roberta; Bolton, David; Matou, Sabine; Ali, Kamela; Tsai, Li-Huei; Krupinski, Jerzy; Petcu, Eugene B.; Montaner, Joan; Al Baradie, Raid; Caccuri, Francesca; Caruso, Arnaldo; Alessandri, Giulio; Kumar, Shant; Rodriguez, Cristina; Martinez-Gonzalez, Jose; Slevin, Mark

    2013-01-01

    Cyclin-dependent kinase-5 (Cdk5) is over-expressed in both neurons and microvessels in hypoxic regions of stroke tissue and has a significant pathological role following hyper-phosphorylation leading to calpain-induced cell death. Here, we have identified a critical role of Cdk5 in cytoskeleton/focal dynamics, wherein its activator, p35, redistributes along actin microfilaments of spreading cells co-localising with p(Tyr15)Cdk5, talin/integrin beta-1 at the lamellipodia in polarising cells. Cdk5 inhibition (roscovitine) resulted in actin-cytoskeleton disorganisation, prevention of protein co-localization and inhibition of movement. Cells expressing Cdk5 (D144N) kinase mutant, were unable to spread, migrate and form tube-like structures or sprouts, while Cdk5 wild-type over-expression showed enhanced motility and angiogenesis in vitro, which was maintained during hypoxia. Gene microarray studies demonstrated myocyte enhancer factor (MEF2C) as a substrate for Cdk5-mediated angiogenesis in vitro. MEF2C showed nuclear co-immunoprecipitation with Cdk5 and almost complete inhibition of differentiation and sprout formation following siRNA knock-down. In hypoxia, insertion of Cdk5/p25-inhibitory peptide (CIP) vector preserved and enhanced in vitro angiogenesis. These results demonstrate the existence of critical and complementary signalling pathways through Cdk5 and p35, and through which coordination is a required factor for successful angiogenesis in sustained hypoxic condition. PMID:24098701

  14. Targeting p35/Cdk5 signalling via CIP-peptide promotes angiogenesis in hypoxia.

    Directory of Open Access Journals (Sweden)

    Alessandra Bosutti

    Full Text Available Cyclin-dependent kinase-5 (Cdk5 is over-expressed in both neurons and microvessels in hypoxic regions of stroke tissue and has a significant pathological role following hyper-phosphorylation leading to calpain-induced cell death. Here, we have identified a critical role of Cdk5 in cytoskeleton/focal dynamics, wherein its activator, p35, redistributes along actin microfilaments of spreading cells co-localising with p(Tyr15Cdk5, talin/integrin beta-1 at the lamellipodia in polarising cells. Cdk5 inhibition (roscovitine resulted in actin-cytoskeleton disorganisation, prevention of protein co-localization and inhibition of movement. Cells expressing Cdk5 (D144N kinase mutant, were unable to spread, migrate and form tube-like structures or sprouts, while Cdk5 wild-type over-expression showed enhanced motility and angiogenesis in vitro, which was maintained during hypoxia. Gene microarray studies demonstrated myocyte enhancer factor (MEF2C as a substrate for Cdk5-mediated angiogenesis in vitro. MEF2C showed nuclear co-immunoprecipitation with Cdk5 and almost complete inhibition of differentiation and sprout formation following siRNA knock-down. In hypoxia, insertion of Cdk5/p25-inhibitory peptide (CIP vector preserved and enhanced in vitro angiogenesis. These results demonstrate the existence of critical and complementary signalling pathways through Cdk5 and p35, and through which coordination is a required factor for successful angiogenesis in sustained hypoxic condition.

  15. Less understood issues: p21(Cip1) in mitosis and its therapeutic potential.

    Science.gov (United States)

    Kreis, N-N; Louwen, F; Yuan, J

    2015-04-01

    p21(Cip1) is a multifunctional protein and a key player in regulating different cellular processes. The transcription of p21 is regulated by p53-dependent and -independent pathways. The expression of p21 is increased in response to various cellular stresses to arrest the cell cycle and ensure genomic stability. p21 has been shown to be a tumor suppressor and an oncogene as well. The function of p21 in mitosis has been proposed but not systematically studied. We have recently shown that p21 binds to and inhibits the activity of Cdk1/cyclin B1, and is important for a fine-tuned mitotic progression. Loss of p21 prolongs the duration of mitosis and results in severe mitotic defects like chromosome segregation and cytokinesis failures promoting consequently genomic instability. Moreover, p21 is dramatically stabilized in mitotic tumor cells upon treatment with mitotic agents like paclitaxel or mitotic kinase inhibitors. Increased p21 is mainly localized in the cytoplasm and associates with cell survival indicating a crucial role of p21 in susceptibility to mitotic agents in tumor cells. In this review we will briefly summarize the structure and general physiological functions as well as regulation of p21, discuss in detail its role in mitosis and its potential to serve as a therapeutic target.

  16. Expression,Purification and Spectral Characterization of p21Waf1/Cip1

    Institute of Scientific and Technical Information of China (English)

    SHI Qiao-yun; ZHENG Yong-chen; REN Jin-song; QU Xiao-gang

    2008-01-01

    p21Wafl/Cip1 ,best known as a broad-specificity inhibitor of cyclin/cyclin-dependent kinase complexes,can interact with various target proteins,and this ability relies on its structural plasticity.Therefore,studies on the structural properties of p21 are very important to understand its structure-function relationship.However,detailed studies on its secondary strcture and biophysical properties have been comparatively sparse.A human p21 gene was cloned into the temperature expression vector pBV220 and transformed into Escherichia coli strain JM109.Recombinant protein was expressed as a non-fusion protein and purified by gel filtration and anion exchange chromatography.The purified protein was verified by Western blot and the functional activity was recognized by pull-down assay.Furthermore,circular dichroisrn,fluorescence spectroscopy,and fluorescence quenching methods were used to characterize the conformational properties of the purified protein.The results indicate that it was largely unstructured under the native solution conditions,and its tryptophan residues were exposed and located in a positively charged microenvironment.This study lays a good foundation for further study of p21 binding to its different partners.

  17. Enzymatic biosensors based on ingá-cipó peroxidase immobilised on sepiolite for TBHQ quantification.

    Science.gov (United States)

    Regina de Oliveira, Tássia; Grawe, Gregory Ferreira; Moccelini, Sally Katiuce; Terezo, Ailton J; Castilho, Marilza

    2014-05-07

    Sepiolite clay mineral was used as a support for the immobilisation of the peroxidase enzyme from ingá-cipó (Inga edulis Mart.) and was used with graphite powder, multi-walled carbon nanotubes (CNTs), mineral oil, and nafion 0.5% (v/v) in the development of a new biosensor for the determination of the antioxidant tert-butylhydroquinone (TBHQ) by square-wave voltammetry (SWV). For the optimisation and application of the biosensor, several parameters were investigated to determine the optimum experimental conditions using SWV. The best performance was obtained using a 0.1 mol L(-1) phosphate buffer solution (pH 7.0), 4.0 × 10(-4) mol L(-1) hydrogen peroxide, a frequency of 50 Hz, a pulse amplitude of 60 mV, and a scan increment of 6 mV. The biosensor showed good repeatability and reproducibility and remained stable for a period of 20 weeks. The analytical curve revealed a linear response range of 1.65 to 9.82 mg L(-1) (r = 0.994) with detection and quantification limits of 0.41 and 1.25 mg L(-1). A recovery study of TBHQ in salad dressing samples yielded values from 99.6-104.8%. The proposed biosensor was successfully used for the determination of TBHQ in commercial salad dressing samples, giving a relative error of 5.4% in relation to the comparative method (chromatographic).

  18. p21WAF1/CIP1 Expression is Differentially Regulated by Metformin and Rapamycin

    Directory of Open Access Journals (Sweden)

    Zoltan Molnar

    2014-01-01

    Full Text Available The mammalian target of rapamycin (mTOR pathway plays an important role in the development of diabetic nephropathy and other age-related diseases. One of the features of DN is the elevated expression of p21WAF1/CIP1. However, the importance of the mTOR signalling pathway in p21 regulation is poorly understood. Here we investigated the effect of metformin and rapamycin on mTOR-related phenotypes in cell lines of epithelial origin. This study reports that metformin inhibits high glucose-induced p21 expression. High glucose opposed metformin in regulating cell size, proliferation, and protein synthesis. These effects were associated with reduced AMPK activation, affecting downstream mTOR signalling. However, the inhibition of the mTOR pathway by rapamycin did not have a negative effect on p21 expression, suggesting that metformin regulates p21 upstream of mTOR. These findings provide support for the hypothesis that AMPK activation may regulate p21 expression, which may have implications for diabetic nephropathy and other age-related pathologies.

  19. Chesapeake Inundation Prediction System (CIPS): A regional prototype for a national problem

    Science.gov (United States)

    Stamey, B.; Smith, W.; Carey, K.; Garbin, D.; Klein, F.; Wang, Hongfang; Shen, J.; Gong, W.; Cho, J.; Forrest, D.; Friedrichs, C.; Boicourt, W.; Li, M.; Koterba, M.; King, D.; Titlow, J.; Smith, E.; Siebers, A.; Billet, J.; Lee, J.; Manning, Douglas R.; Szatkowski, G.; Wilson, D.; Ahnert, P.; Ostrowski, J.

    2007-01-01

    Recent Hurricanes Katrina and Isabel, among others, not only demonstrated their immense destructive power, but also revealed the obvious, crucial need for improved storm surge forecasting and information delivery to save lives and property in future storms. Current operational methods and the storm surge and inundation products do not adequately meet requirements needed by Emergency Managers (EMs) at local, state, and federal levels to protect and inform our citizens. The Chesapeake Bay Inundation Prediction System (CIPS) is being developed to improve the accuracy, reliability, and capability of flooding forecasts for tropical cyclones and non-tropical wind systems such as nor'easters by modeling and visualizing expected on-land storm-surge inundation along the Chesapeake Bay and its tributaries. An initial prototype has been developed by a team of government, academic and industry partners through the Chesapeake Bay Observing System (CBOS) of the Mid-Atlantic Coastal Ocean Observing Regional Association (MACOORA) within the Integrated Ocean Observing System (IOOS). For demonstration purposes, this initial prototype was developed for the tidal Potomac River in the Washington, DC metropolitan area. The preliminary information from this prototype shows great potential as a mechanism by which NOAA National Weather Service (NWS) Forecast Offices (WFOs) can provide more specific and timely forecasts of likely inundation in individual localities from significant storm surge events. This prototype system has shown the potential to indicate flooding at the street level, at time intervals of an hour or less, and with vertical resolution of one foot or less. This information will significantly improve the ability of EMs and first responders to mitigate life and property loss and improve evacuation capabilities in individual communities. This paper provides an update and expansion of the initial prototype that was presented at the Oceans 2006 MTS/IEEE Conference in Boston, MA

  20. p21(WAF1/CIP1 RNA expression in highly HIV-1 exposed, uninfected individuals.

    Directory of Open Access Journals (Sweden)

    Joshua Herbeck

    Full Text Available Some individuals remain HIV-1 antibody and PCR negative after repeated exposures to the virus, and are referred to as HIV-exposed seronegatives (HESN. However, the causes of resistance to HIV-1 infection in cases other than those with a homozygous CCR5Δ32 deletion are unclear. We hypothesized that human p21WAF1/CIP1 (a cyclin-dependent kinase inhibitor could play a role in resistance to HIV-1 infection in HESN, as p21 expression has been associated with suppression of HIV-1 in elite controllers and reported to block HIV-1 integration in cell culture. We measured p21 RNA expression in PBMC from 40 HESN and 40 low exposure HIV-1 seroconverters (LESC prior to their infection using a real-time PCR assay. Comparing the 20 HESN with the highest exposure risk (median = 111 partners/2.5 years prior to the 20 LESC with the lowest exposure risk (median = 1 partner/2.5 years prior, p21 expression trended higher in HESN in only one of two experiments (P = 0.11 vs. P = 0.80. Additionally, comparison of p21 expression in the top 40 HESN (median = 73 partners/year and lowest 40 LESC (median = 2 partners/year showed no difference between the groups (P = 0.84. There was a weak linear trend between risk of infection after exposure and increasing p21 gene expression (R2 = 0.02, P = 0.12, but again only in one experiment. Hence, if p21 expression contributes to the resistance to viral infection in HESN, it likely plays a minor role evident only in those with extremely high levels of exposure to HIV-1.

  1. p21cip1在肿瘤生物治疗中的地位与作用%Status and role of p21cip1 for cancer biological therapy

    Institute of Scientific and Technical Information of China (English)

    陈文虎; 毛伟敏

    2009-01-01

    肿瘤是一类细胞周期性疾病,将细胞周期调控因子导入肿瘤细胞可代替或补偿缺陷的基因以抑制肿瘤的异常增生或逆转基因型.p21cip1是一种重要的细胞周期负调控蛋白,它参与细胞的生长、分化、衰老及死亡过程,同时义与肿瘤发生密切相关.就p21在肿瘤发生、发展中的作用及在肿瘤生物治疗中的主要策略作一综述.%Cancer is frequently considered to be a disease of cell cycle. Recently, it is reported that the transfer of functional ceil cycle regulators into caneer cells via vectors has the potential to intervene tumorigenesis development. This therapeutic strategy might allow cancer cells to regain missing or dysfunctional gene expression due to inhibiting aberrant proliferation or restoring phenotypes defects. As an important negative effecter of cell cycle progression, p21cip1 not only plays central roles in cell proliferation,differentiation, senescence and apoptosis, but also associates with tumor regression. In this review, we will mainly focus on discussing some recent progress in novel strategies for cancer biological therapy, related with the functions of p21cip1 in cell proliferation and apoptosis during tumorigenesis.

  2. In vitro effect of p21WAF-1/CIP1 gene on growth of human glioma cells mediated by EGFR targeted non-viral vector GE7 system

    Institute of Scientific and Technical Information of China (English)

    陈永新; 许秀兰; 张光霁; 王韦; 金海英; 卢亦成; 朱诚; 顾健人

    2003-01-01

    Objective: To construct the EGFR targeted non-viral vector GE7 system and explore the in vitro effect of p21WAF-1/CIP1 gene on growth of human glioma cells mediated by the GE7 system. Methods: The EGFR targeted non-viral vector GE7 gene delivery system was constructed. The malignant human glioma cell line U251MG was transfected in vitro with β-galactosidase gene(reporter gene) and p21WAF-1/CIP1 gene (therapeutic gene) using the GE7 system. By means of X-gal staining, MTS and FACS, the transfection efficiency of exogenous gene and apoptosis rate of tumor cells were examined. The expression of p21WAF-1/CIP1 gene in transfected U251MG cell was examined by immunohistochemistry staining. Results: The highest transfer rate of exogenous gene was 70%. After transfection with p21WAF-1/CIP1 gene, the expression of WAF-1 increased remarkably and steadily; the growth of U251MG cells were inhibited evidently. FACS examination showed G1 arrest. The average apoptosis rate was 25.2%. Conclusion: GE7 system has the ability to transfer exogenous gene to targeted cells efficiently, and expression of p21WAF-1/CIP1 gene can induce apoptosis of glioma cell and inhibit its growth.

  3. p21Cip1 Protection against Hyperoxia Requires Bcl-XL and Is Uncoupled from Its Ability to Suppress Growth

    OpenAIRE

    2006-01-01

    The cyclin-dependent kinase inhibitor p21Cip1/Waf1/Sdi1 protects the lung against hyperoxia, but the mechanism of protection remains unclear because loss of p21 does not lead to aberrant cell proliferation. Because some members of the Bcl-2 gene family have been implicated in hyperoxia-induced cell death, the current study investigated their expression as well as p21-dependent growth suppression and cytoprotection. Conditional overexpression of full-length p21, its amino-terminal cyclin-bindi...

  4. SoCIP 2011成功落幕,S2C众多新品期待爆发

    Institute of Scientific and Technical Information of China (English)

    韩霜

    2011-01-01

    由S2C(思尔芯)公司主办的第四届SoCIP 2011研讨展览会于2011年5月24日和26日分别在上海及北京举行。今年共有14家国内外厂商参与了展览会的展出,包括Allegro、AndesTechnology、C~*Core、CAST、CosmicCircuits、InPA、IPGoal、NorthwestLogic、PerfectVIPs、SpringSoft、Tensilica、Transwitch、Vivante与S2C。来自以上公司的相关人士通过一

  5. Induction of anergy in Th1 cells associated with increased levels of cyclin-dependent kinase inhibitors p21Cip1 and p27Kip1.

    Science.gov (United States)

    Jackson, S K; DeLoose, A; Gilbert, K M

    2001-01-15

    Th1 cells exposed to Ag and the G(1) blocker n-butyrate in primary cultures lose their ability to proliferate in Ag-stimulated secondary cultures. The ability of n-butyrate to induce anergy in Ag-stimulated, but not resting, Th1 cells was shown here to be blocked by cycloheximide. Subsequent experiments to delineate the nature of the protein apparently required for n-butyrate-induced Th1 cell anergy focused on the role of cyclin-dependent kinase (cdk) inhibitors p21(Cip1) and p27(Kip1). Normally, entry into S phase by Th1 cells occurs around 24 h after Ag stimulation and corresponds with relatively low levels of both p21(Cip1) and p27(Kip1). However, unlike control Th1 cells, anergic Th1 cells contained high levels of both p21(Cip1) and p27(Kip1) when examined 24 h after Ag stimulation. The increase in p21(Cip1) observed in Ag-stimulated anergic Th1 cells appeared to be initiated in primary cultures. In contrast, the increase in p27(Kip1) observed in these anergic Th1 cells appears to represent a re-expression of the protein much earlier than control cells following Ag stimulation in secondary cultures. The anergic Th1 cells contained functionally active cdk inhibitors capable of inhibiting the activity of both endogenous and exogenous cdks. Consequently, it appears that n-butyrate-induced anergy in Th1 cells correlated with the up-regulation of p21(Cip1) and perhaps the downstream failure to maintain low levels of p27(Kip1). Increased levels of both p21(Cip1) and p27(Kip1) at the end of G(1) could prevent cdk-mediated entry into S phase, and thus help maintain the proliferative unresponsiveness found in the anergic Th1 cells.

  6. CDK inhibitors, p21{sup Cip1} and p27{sup Kip1}, participate in cell cycle exit of mammalian cardiomyocytes

    Energy Technology Data Exchange (ETDEWEB)

    Tane, Shoji; Ikenishi, Aiko; Okayama, Hitomi; Iwamoto, Noriko [School of Life Sciences, Faculty of Medicine, Tottori University, Yonago 683-8503 (Japan); Nakayama, Keiichi I. [Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582 (Japan); Takeuchi, Takashi, E-mail: takeuchi@med.tottori-u.ac.jp [School of Life Sciences, Faculty of Medicine, Tottori University, Yonago 683-8503 (Japan)

    2014-01-17

    Highlights: •Expression of p21 and p27 in the hearts showed a peak during postnatal stages. •p21 and p27 bound to cyclin E, cyclin A and CDK2 in the hearts at postnatal stages. •Cardiomyocytes in both KO mice showed failure in the cell cycle exit at G1-phase. •These data show the first apparent phenotypes in the hearts of Cip/Kip KO mice. -- Abstract: Mammalian cardiomyocytes actively proliferate during embryonic stages, following which cardiomyocytes exit their cell cycle after birth. The irreversible cell cycle exit inhibits cardiac regeneration by the proliferation of pre-existing cardiomyocytes. Exactly how the cell cycle exit occurs remains largely unknown. Previously, we showed that cyclin E- and cyclin A-CDK activities are inhibited before the CDKs levels decrease in postnatal stages. This result suggests that factors such as CDK inhibitors (CKIs) inhibit CDK activities, and contribute to the cell cycle exit. In the present study, we focused on a Cip/Kip family, which can inhibit cyclin E- and cyclin A-CDK activities. Expression of p21{sup Cip1} and p27{sup Kip1} but not p57{sup Kip2} showed a peak around postnatal day 5, when cyclin E- and cyclin A-CDK activities start to decrease. p21{sup Cip1} and p27{sup Kip1} bound to cyclin E, cyclin A and CDK2 at postnatal stages. Cell cycle distribution patterns of postnatal cardiomyocytes in p21{sup Cip1} and p27{sup Kip1} knockout mice showed failure in the cell cycle exit at G1-phase, and endoreplication. These results indicate that p21{sup Cip1} and p27{sup Kip} play important roles in the cell cycle exit of postnatal cardiomyocytes.

  7. Assessment of the potential suitability of selected commercially available enzymes for cleaning-in-place (CIP) in the dairy industry.

    Science.gov (United States)

    Boyce, Angela; Piterina, Anna V; Walsh, Gary

    2010-10-01

    The potential suitability of 10 commercial protease and lipase products for cleaning-in-place (CIP) application in the dairy industry was investigated on a laboratory scale. Assessment was based primarily on the ability of the enzymes to remove an experimentally generated milk fouling deposit from stainless steel (SS) panels. Three protease products were identified as being most suitable for this application on the basis of their cleaning performance at 40 °C, which was comparable to that of the commonly used cleaning agent, 1% NaOH at 60 °C. This was judged by quantification of residual organic matter and protein on the SS surface after cleaning and analysis by laser scanning confocal microscopy (LSCM). Enzyme activity was removed/inactivated under conditions simulating those normally undertaken after cleaning (rinsing with water, acid circulation, sanitation). Preliminary process-scale studies strongly suggest that enzyme-based CIP achieves satisfactory cleaning at an industrial scale. Cost analysis indicates that replacing caustic-based cleaning procedures with biodegradable enzymes operating at lower temperatures would be economically viable. Additional potential benefits include decreased energy and water consumption, improved safety, reduced waste generation, greater compatibility with wastewater treatment processes and a reduction in the environmental impact of the cleaning process.

  8. The Use of Ion Chromatography for the Determination of Clean-In-Place-200 (CIP-200 Detergent Traces

    Directory of Open Access Journals (Sweden)

    Héctor Colón

    2006-01-01

    Full Text Available Anion chromatography with conductivity detection was chosen as the analytical technique for the development of a cleaning validation method for clean-in-place (CIP detergents. The method was developed and validated for the determination of traces of the detergent CIP-200. It was shown to be linear with a squared correlation coefficient (r2 of 0.9999 and the accuracy experiments presented average recoveries of 88.2% (area response factor from stainless steel surfaces. The repeatability was found to be 1.6% and an intermediate precision of 1.9% across the range. The method was also shown to be sensitive with an average Detection Limit (DL of 0.23 ppm and a Quantitation Limit (QL of 0.70 ppm based on the amount of phosphate in the detergent sample. The phosphate signal was well resolved from typical ions encountered in water samples or any other interference presented from swabs and surfaces. The method was applied to cleaning validation samples and proved to be suitable for rapid and reliable quality control.

  9. Parâmetros fitossociológicos de um cerrado no Parque Nacional Da Serra Do Cipó, MG Phytosociological parameters of a cerrado in "Serra Do Cipó" national park, Minas Gerais, Brazil

    Directory of Open Access Journals (Sweden)

    João Augusto Alves Meira Neto

    2002-10-01

    Full Text Available A riqueza de espécies e os fatores determinantes da ocorrência da vegetação de cerrado há muito têm despertado o interesse de pesquisadores. Muitos cerrados protegidos em unidades de conservação ainda não foram investigados florística e estruturalmente. Neste trabalho foi realizado um levantamento florístico e fitossociológico no Parque Nacional da Serra do Cipó (19º22'01''S e 43º37'10''W. Foram instaladas 12 parcelas de 150 m² e foram amostrados todos os indíviduos lenhosos com circunferência do caule à altura do solo maior ou igual a 10 cm. Foram relacionadas 44 espécies de 37 gêneros e 30 famílias. Entre estas, Leguminosae Caesalpinioideae, Leguminosae Mimosoideae e Guttiferae, com três espécies cada, foram as mais ricas. As espécies mais importantes (VI foram Hymenaea stigonocarpa, Allagoptera campestris, Diospyros hispida, Rapanea guianensis e Piptocarpha rotundifolia.The diversity of species and the factors determining the occurrence of cerrado vegetation have long called the attention of researchers. Many protected cerrados in Conservation Units have not been floristically and structurally studied. This work conducted a floristic and phytosociological survey of the Parque Nacional da "Serra do Cipó" ("Serra do Cipó" National Park (19º22'01''S and 43º37'10''W. Twelve quadrats of 150 m² were established and all individuals with a stem circumference at ground level larger than or equal to 10 cm were sampled. A total of 44 species of 37 genera and 30 families were found. The richest families were Leguminosae Caesalpinioideae, Leguminosae Mimosoideae and Guttiferae, with three species each. The most important species (VI were Hymenaea stigonocarpa, Allagoptera campestris, Diospyros hispida, Rapanea guianensis and Piptocarpha rotundifolia.

  10. reliability analysis of a two span floor designed according to ...

    African Journals Online (AJOL)

    user

    The Structural analysis and design of the timber floor system was carried out using deterministic approach ... The cell structure of hardwoods is more complex than ..... [12] BS EN -1-1: Eurocode 5: Design of Timber Structures, Part. 1-1.

  11. 5-Aza-2'-deoxycytidine Activates the p53/p21waf1/Cip1 Pathway to Inhibit Cell Proliferation

    Institute of Scientific and Technical Information of China (English)

    Wei-GuoZhu; TheresaHileman; YangKe; PeichangWang; ShaoliLu; WenruiDuan; ZunyanDai; TanjunTong; MiguelA.Villalona-Calero; ChristophPlass; GregoryA.Otterson

    2005-01-01

    In addition to its demethylating function, 5-aza-2'-de- oxycytidine (5-aza-CdR) also plays an important role in inducing cell cycle arrest, differentiation, and cell death. However, the mechanism by which 5-aza-CdR in. duces antineoplastic activity is not clear. In this study, we found that 5-aza-CdR at limited concentrations(0.01-5μM) induces inhibition of cell proliferation as well as increased p53/p21waf1/Cip1 expression in A549 cells (wild-type p53) but not in H1299 (p53-null) and H719 cells (p53 mutant). The p53-dependent p21wafa/Cip1 expression induced by 5-aza-CdR was not seen in A549 cells transfected with the wild-type human papilloma virus type-16 E6 gene that induces p53 degradation. Furthermore, deletion analysis and site-directed mutagenesis of the p21 promoter reveals that 5-aza-CdR induces p21wafa/Cip1 expression through two p53 binding sites in the p21 promoter. Finally, 5-aza-CdR-induced p21waf1/cip1 expression was dependent on DNA damage but not on DNA demethylation as demonstrated by comet assay and bisulfite sequencing, respectively. Our data provide useful clues for judging the therapeutic efficacy of 5-aza-CdR in the treatment of human cancer cells.

  12. Oxidative stress biomarkers in different tissues of rainbow trout (Oncorhynchus mykiss exposed to Disinfectant-CIP formulated with peracetic acid and hydrogen peroxide

    Directory of Open Access Journals (Sweden)

    Tkachenko Halyna

    2014-09-01

    Full Text Available The aim of study was to determine the effects of exposure to the product DEZYNFEKTANT-CIP (Eng. - Disinfectant-CIP, which is formulated with peracetic acid and hydrogen peroxide, on oxidative stress biomarkers (lipid peroxidation (LPO levels and the carbonyl content of oxidatively modified proteins and antioxidant defenses (superoxide dismutase (SOD, catalase (CAT, glutathione reductase (GR, glutathione peroxidase (GPx, total antioxidant capacity in muscle, gill, hepatic, and cardiac tissues of rainbow trout, Oncorhynchus mykiss (Walbaum. LPO and carbonyl contents changed with tissue type. Exposure to Disinfectant-CIP led to a significant decrease in LPO in muscle tissues and carbonyl content in muscle and gill tissues. The inhibition of SOD and CAT activity in muscle, hepatic, and cardiac tissues was observed probably because of increased oxidative stress during disinfection; however, hepatic and cardiac GPx activity increased in an attempt to counteract oxidative stress. We suggest that oxidative stress during the oxidation of peracetic acid and hydrogen peroxide could be counteracted by the antioxidant system in trout tissues. Correlative analysis between oxidative stress biomarkers and antioxidant defense confirms the pivotal role of SOD and CAT against CIP-induced oxidative stress

  13. 1,25(OH)2D3 inhibits the progression of hepatocellular carcinoma via downregulating HDAC2 and upregulating P21(WAFI/CIP1).

    Science.gov (United States)

    Huang, Jian; Yang, Guozhen; Huang, Yunzhu; Kong, Weiying; Zhang, Shu

    2016-02-01

    Vitamin D, termed 1,25(OH)2D3 in it's active form, activity is associated with a reduced risk of hepatocellular carcinoma (HCC) and is an important immune regulator. However, the detail molecular mechanisms underlying the effects of 1,25(OH)2D3 on the progression of HCC are widely unknown. Histone deacetwylase 2 (HDAC2) is usually expressed at high levels in tumors, and its downregulation leads to high expression levels of cell cycle components, including p21(WAF1/Cip1), a well-characterized modulator, which is critical in cell senescence and apoptosis. The present study investigated whether vitamin D inhibits HCC via the regulation of HDAC2 and p21(WAF1/Cip1). Firstly, the toxic concentrations of 1,25(OH)2D3 were determined, according to trypan blue and [(3)H]thymidine incorporation assays. Secondly, HCC cells lines were treated with different concentrations of 1,25(OH)2D3. The expression of HDAC2 was either silenced via short hairpin (sh)RNA or induced by transfection of plasmids expressing the HDAC2 gene in certain HCC cells. Finally the mRNA and protein levels of HDAC2 and p21(WAF1/Cip1) were measured using reverse transcription-quantitative polymerase chain reaction and western blot analyses. The results revealed that 1,25(OH)2D3 treatment reduced the expression of HDAC2 and increased the expression of p21(WAF1/Cip1), in a dose-dependent manner, resulting in the reduction of HCC growth. Elevated levels of HDAC2 reduced the expression of p21(WAF1/Cip1), resulting in an increase in HCC growth. HDAC2 shRNA increased the expression of p21(WAF1/Cip1), resulting in reduction in HCC growth. Thus, 1,25(OH)2D3 exerted antitumorigenic effects through decreasing the expression levels of HDAC2 and increasing the expression of p21(WAF1/Cip1), which inhibited the development of HCC and may indicate the possible underlying mechanism. These results suggest that vitamin D3 may be developed as a potential drug for effective therapy in the treatment of HCC.

  14. THE EXPRESSION AND CLINICAL SIGNIFICANCE OF P21 (WAF1/CIP1)AND CYCLIN D1 PROTEIN IN COLORECTAL CARCINOMA

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Objective To study the effect of P21 (WAF1/CIP1) and cyclin D1 and their relationship in colorec- tal carcinoma. Methods The expression of P21 and cyclin D1 was studied in 40 colorectal carcinoma and 10 normal tissues using S-P immunohistochemical technique. Results Decreased expression of P12 and overexpression of cyclin D1 were revealed in colorectal carcinoma. Decreased expression of P21 was related to lymph node metastasis. No cor- relation was found between cyclin D1 and clinicopathological parameters. Conclusion Decreased expression of P21 and overexpression of cyclin D1 may be involved in colorectal tumorigenesis,and were associated with poor prognosis. No correlation was found between P21 and cyclin D1 in colorectai carcinoma.

  15. A numerical study of tsunami wave impact and run-up on coastal cliffs using a CIP-based model

    Science.gov (United States)

    Zhao, Xizeng; Chen, Yong; Huang, Zhenhua; Hu, Zijun; Gao, Yangyang

    2017-05-01

    There is a general lack of understanding of tsunami wave interaction with complex geographies, especially the process of inundation. Numerical simulations are performed to understand the effects of several factors on tsunami wave impact and run-up in the presence of gentle submarine slopes and coastal cliffs, using an in-house code, a constrained interpolation profile (CIP)-based model. The model employs a high-order finite difference method, the CIP method, as the flow solver; utilizes a VOF-type method, the tangent of hyperbola for interface capturing/slope weighting (THINC/SW) scheme, to capture the free surface; and treats the solid boundary by an immersed boundary method. A series of incident waves are arranged to interact with varying coastal geographies. Numerical results are compared with experimental data and good agreement is obtained. The influences of gentle submarine slope, coastal cliff and incident wave height are discussed. It is found that the tsunami amplification factor varying with incident wave is affected by gradient of cliff slope, and the critical value is about 45°. The run-up on a toe-erosion cliff is smaller than that on a normal cliff. The run-up is also related to the length of a gentle submarine slope with a critical value of about 2.292 m in the present model for most cases. The impact pressure on the cliff is extremely large and concentrated, and the backflow effect is non-negligible. Results of our work are highly precise and helpful in inverting tsunami source and forecasting disaster.

  16. Continuous auditing & continuous monitoring : Continuous value?

    NARCIS (Netherlands)

    van Hillo, Rutger; Weigand, Hans; Espana, S; Ralyte, J; Souveyet, C

    2016-01-01

    Advancements in information technology, new laws and regulations and rapidly changing business conditions have led to a need for more timely and ongoing assurance with effectively working controls. Continuous Auditing (CA) and Continuous Monitoring (CM) technologies have made this possible by obtain

  17. Continuous auditing & continuous monitoring : Continuous value?

    NARCIS (Netherlands)

    van Hillo, Rutger; Weigand, Hans; Espana, S; Ralyte, J; Souveyet, C

    2016-01-01

    Advancements in information technology, new laws and regulations and rapidly changing business conditions have led to a need for more timely and ongoing assurance with effectively working controls. Continuous Auditing (CA) and Continuous Monitoring (CM) technologies have made this possible by obtain

  18. Continuous auditing & continuous monitoring : Continuous value?

    NARCIS (Netherlands)

    van Hillo, Rutger; Weigand, Hans; Espana, S; Ralyte, J; Souveyet, C

    2016-01-01

    Advancements in information technology, new laws and regulations and rapidly changing business conditions have led to a need for more timely and ongoing assurance with effectively working controls. Continuous Auditing (CA) and Continuous Monitoring (CM) technologies have made this possible by

  19. The effects of two polymorphisms on p21cip1 function and their association with Alzheimer's disease in a population of European descent.

    Directory of Open Access Journals (Sweden)

    Sharon C Yates

    Full Text Available With the exception of ApoE4, genome-wide association studies have failed to identify strong genetic risk factors for late-onset Alzheimer's disease, despite strong evidence of heritability, suggesting that many low penetrance genes may be involved. Additionally, the nature of the identified genetic risk factors and their relation to disease pathology is also largely obscure. Previous studies have found that a cancer-associated variant of the cell cycle inhibitor gene p21cip1 is associated with increased risk of Alzheimer's disease. The aim of this study was to confirm this association and to elucidate the effects of the variant on protein function and Alzheimer-type pathology. We examined the association of the p21cip1 variant with Alzheimer's disease and Parkinson's disease with dementia. The genotyping studies were performed on 719 participants of the Oxford Project to Investigate Memory and Ageing, 225 participants of a Parkinson's disease DNA bank, and 477 participants of the Human Random Control collection available from the European Collection of Cell Cultures. The post mortem studies were carried out on 190 participants. In the in-vitro study, human embryonic kidney cells were transfected with either the common or rare p21cip1 variant; and cytometry was used to assess cell cycle kinetics, p21cip1 protein expression and sub-cellular localisation. The variant was associated with an increased risk of Alzheimer's disease, and Parkinson's disease with dementia, relative to age matched controls. Furthermore, the variant was associated with an earlier age of onset of Alzheimer's disease, and a more severe phenotype, with a primary influence on the accumulation of tangle pathology. In the in-vitro study, we found that the SNPs reduced the cell cycle inhibitory and anti-apoptotic activity of p21cip1. The results suggest that the cancer-associated variant of p21cip1 may contribute to the loss of cell cycle control in neurons that may lead to

  20. 2008 Mississippi Curriculum Framework: Postsecondary Pharmacy Technology. (Program CIP: 51.0805 - Pharmacy Technician/Assistant)

    Science.gov (United States)

    Juergens, John; Sanders, Marsha

    2008-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  1. 2011 Mississippi Curriculum Framework: Postsecondary Dental Hygiene Technology. (Program CIP: 51.0602 - Dental Hygienist)

    Science.gov (United States)

    Alexander, Nick; Dunn, Catherine; Hill, Stanley; Lindsay, William

    2011-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  2. 2010 Mississippi Curriculum Framework: Postsecondary Clothing and Textile Services. (Program CIP-19.0905)

    Science.gov (United States)

    Redfield, Verlene

    2010-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  3. 2010 Mississippi Curriculum Framework: Postsecondary Paralegal Technology. (Program CIP: 22.0302 - Legal Assistant/Paralegal)

    Science.gov (United States)

    Cox, Janet; Dinkins, Shivochie L.; Dozier, Darleen; Ford, Kathryn; Hinton, Amy; McDavid, Stephan; Phifer-Starks, Kim D.; Winston, Felicia

    2010-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  4. 2006 Mississippi Curriculum Framework: Postsecondary Paralegal Technology. (Program CIP: 22.0302 - Legal Assistant/Paralegal)

    Science.gov (United States)

    Hinton, Amy; Phifer-Starks, Kim D.; Nelson, LaTricia

    2006-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  5. 2009 Mississippi Curriculum Framework: Postsecondary Occupational Safety and Health Technology. Program CIP: 15.0701

    Science.gov (United States)

    Flowers, Walter M.

    2009-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  6. 2011 Mississippi Curriculum Framework: Postsecondary Occupational Therapy Assistant. (Program CIP: 51.0803 - Occupational Therapist Assistant)

    Science.gov (United States)

    Hager, Sherry; Pulver, Tim; Chittom, Suzanne

    2011-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  7. 2011 Mississippi Curriculum Framework: Postsecondary Paramedic. (Program CIP: 51.0904 - Emergency Medical Technology/Technician)

    Science.gov (United States)

    Briscoe, Lisa; Bryant, Katrina; Deschamp, Clyde; Galtelli, Mark; Glasson, Kristi; Hall, David; Hood, Brenda; Mahaffey, Libby; McBryde, John; Read, John; Shirley, Gary

    2011-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  8. 2009 Mississippi Curriculum Framework: Postsecondary Respiratory Care Technology. (Program CIP: 51.0908 - Respiratory Care Practitioner)

    Science.gov (United States)

    Anderson, Lori; Brooks, Anthony; Miller, Shirley; Newell, Jim; Prince, Beverly; Smith, Jacqueline; Smith, Lynn; Ware, Chris

    2009-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  9. 2007 Mississippi Curriculum Framework: Postsecondary Occupational Therapy Assistant. (Program CIP: 51.0803 - Occupational Therapist Assistant)

    Science.gov (United States)

    Hager, Sherry; Pulver, Tim; Chittom, Suzanne

    2007-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  10. 2006 Mississippi Curriculum Framework: Postsecondary Physical Therapist Assistant. (Program CIP: 51.0806 - Physical Therapist Assistant)

    Science.gov (United States)

    Hester, Tom; Latham, Lisa; Crowson, Patti; Ennis, Kimberly

    2006-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  11. 2010 Mississippi Curriculum Framework: Postsecondary Physical Therapist Assistant. (Program CIP: 51.0806 - Physical Therapist Assistant)

    Science.gov (United States)

    Hester, Tom; Chapman, Pamela; Crowson, Patti; Ennis, Kimberly

    2010-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  12. Design optimization of continuous partially prestressed concrete beams

    Science.gov (United States)

    Al-Gahtani, A. S.; Al-Saadoun, S. S.; Abul-Feilat, E. A.

    1995-04-01

    An effective formulation for optimum design of two-span continuous partially prestressed concrete beams is described in this paper. Variable prestressing forces along the tendon profile, which may be jacked from one end or both ends with flexibility in the overlapping range and location, and the induced secondary effects are considered. The imposed constraints are on flexural stresses, ultimate flexural strength, cracking moment, ultimate shear strength, reinforcement limits cross-section dimensions, and cable profile geometries. These constraints are formulated in accordance with ACI (American Concrete Institute) code provisions. The capabilities of the program to solve several engineering problems are presented.

  13. 27-day solar forcing of mesospheric temperature, water vapor and polar mesospheric clouds from the AIM SOFIE and CIPS satellite experiments

    Science.gov (United States)

    Thomas, Gary; Thurairajah, Brentha; von Savigny, Christian; Hervig, Mark; Snow, Martin

    2016-04-01

    Solar cycle variations of ultraviolet radiation have been implicated in the 11-year and 27-day variations of Polar Mesospheric Cloud (PMC) properties. Both of these variations have been attributed to variable solar ultraviolet heating and photolysis, but no definitive studies of the mechanisms are available. The solar forcing issue is critical toward answering the broader question of whether PMC's have undergone long-term changes, and if so, what is the nature of the responsible long-term climate forcings? One of the principal goals of the Aeronomy of Ice in the Mesosphere satellite mission was to answer the question: "How does changing solar irradiance affect PMCs and the environment in which they form?" We describe an eight-year data set from the AIM Solar Occultation for Ice Experiment (SOFIE) and the AIM Cloud Imaging and Particle Size (CIPS) experiment. Together, these instruments provide high-precision measurements of high-latitude summertime temperature (T), water vapor (H2O), and PMC ice properties for the period 2007-present. The complete temporal coverage of the summertime polar cap region for both the primary atmospheric forcings of PMC (T and H2O), together with a continually updated time series of Lyman-alpha solar irradiance, allows an in-depth study of the causes and effects of 27-day PMC variability. The small responses of these variables, relative to larger day-to-day changes from gravity waves, tides, inter-hemispheric coupling, etc. require a careful statistical analysis to isolate the solar influence. We present results for the 27-day responses of T, H2O and PMC for a total of 15 PMC seasons, (30 days before summer solstice to 60 days afterward, for both hemispheres). We find that the amplitudes and phase relationships are not consistent with the expected mechanisms of solar UV heating and photolysis - instead we postulate a primarily dynamical response, in which a periodic vertical wind heats/cools the upper mesosphere, and modulates PMC

  14. Gambogic acid induces growth inhibition and differentiation via upregulation of p21waf1/cip1 expression in acute myeloid leukemia cells.

    Science.gov (United States)

    Chen, Yan; Hui, Hui; Li, Zheng; Wang, Hong-Mei; You, Qi-Dong; Lu, Na

    2014-10-01

    Gambogic acid (GA) is the major active ingredient of gamboges, a brownish to orange resin product from Garcinia hanburyi tree in Southeast Asia. This compound exhibits anti-cancer effect on solid tumors. In this study, we investigated the effects of GA on the growth and differentiation of acute myeloid leukemia cells by growth-inhibition detection, morphological changes observation, nitroblue tetrazolium reduction, and the expression of the relative cell-surface differentiation markers. The results showed that GA could inhibit cell growth and promote differentiation in U937 and HL-60 cells. In addition, GA upregulated the expression of p21waf1/cip1 in the two cell lines. Finally, downregulating the p21waf1/cip1 expression with small interfering RNA partially blocked GA-induced cell growth inhibition and differentiation. These results of this study revealed that GA may be used as one of the investigational drugs for acute myeloid leukemia.

  15. Pre-test simulation of the CIP3-1 experiment of the CABRI water loop with FRAPCON-3.4a and FRAPTRAN 1.4 codes; Simulacion pre-test del experimento CIP-1 del proyecto CABRI Lazo de agua con los codigos FRAPCON-3,4 y FRAPTRAN 1.4

    Energy Technology Data Exchange (ETDEWEB)

    Vallejo, I.; Huguet, A.; Herranz, L. E.

    2010-07-01

    The main objective of this paper is to estimate the thermomechanical behavior expected of the bar part during the reactivity insertion CIP3-1 experiment, that will be done in the new CABRI facility. these pre-test calculations provide an experimental support to find out about the results expected according to the current capabilities of simulation codes.

  16. Involvement of p21Waf1/Cip1 cleavage during roscovitine-induced apoptosis in non-small cell lung cancer cells.

    Science.gov (United States)

    Zhang, Tao; Jiang, Tao; Zhang, Feng; Li, Chen; Zhou, Yong-An; Zhu, Yi-Fang; Li, Xiao-Fei

    2010-01-01

    Roscovitine, a cyclin-dependent kinases (CDKs) inhibitor, has been reported to have anti-tumor effects in some cancer cell lines by inducing apoptosis. However, the exact underlying mechanisms are not fully understood. Here, we report that roscovitine induces expression and cleavage of the universal CDK inhibitor p21Waf1/Cip1 in non-small cell lung cancer (NSCLC) A549 cells in a dose-dependent manner. Western blots of roscovitine-treated cells undergoing apoptosis consistently demonstrated a 15 kDa band that was not detected in control cultures. CDK2 activity and PCNA expression were repressed with increasing dose of roscovitine. Accompanying these molecular changes was a progressive arrest of G2 phase and decreasing of 5-bromo-2-deoxyuridine (Brdu) incorporation of S phase cells. Caspase-3 inhibitor z-DEVD-fmk almost completely abolished roscovitine-induced apoptosis, as well as the appearance of 15 kDa band, indicating that p21Waf1/Cip1 cleavage was mediated by caspase-3 activity. Furthermore, this band was predominant in the floating apoptotic cells, while weakened in the adherent cells which were vital and pre-apoptotic. We also showed that roscovitine induced an enhanced expression of gamma-H2AX, which was blocked by caspase-3 inhibition, suggesting that p21Waf1/Cip1 cleavage may interfere with DNA repair, leading to increased frequency of double strand breaks (DSBs) and enhanced apoptosis. Here we show, for the first time, that p21Waf1/Cip1 cleavage, which is mediated by caspase-3 activity, is involved in roscovitine-induced apoptosis.

  17. p27Kip1 and p21Cip1 collaborate in the regulation of transcription by recruiting cyclin-Cdk complexes on the promoters of target genes.

    Science.gov (United States)

    Orlando, Serena; Gallastegui, Edurne; Besson, Arnaud; Abril, Gabriel; Aligué, Rosa; Pujol, Maria Jesus; Bachs, Oriol

    2015-08-18

    Transcriptional repressor complexes containing p130 and E2F4 regulate the expression of genes involved in DNA replication. During the G1 phase of the cell cycle, sequential phosphorylation of p130 by cyclin-dependent kinases (Cdks) disrupts these complexes allowing gene expression. The Cdk inhibitor and tumor suppressor p27(Kip1) associates with p130 and E2F4 by its carboxyl domain on the promoters of target genes but its role in the regulation of transcription remains unclear. We report here that p27(Kip1) recruits cyclin D2/D3-Cdk4 complexes on the promoters by its amino terminal domain in early and mid G1. In cells lacking p27(Kip1), cyclin D2/D3-Cdk4 did not associate to the promoters and phosphorylation of p130 and transcription of target genes was increased. In late G1, these complexes were substituted by p21(Cip1)-cyclin D1-Cdk2. In p21(Cip1) null cells cyclin D1-Cdk2 were not found on the promoters and transcription was elevated. In p21/p27 double null cells transcription was higher than in control cells and single knock out cells. Thus, our results clarify the role of p27(Kip1) and p21(Cip1) in transcriptional regulation of genes repressed by p130/E2F4 complexes in which p27(Kip1) and p21(Cip1) play a sequential role by recruiting and regulating the activity of specific cyclin-Cdk complexes on the promoters.

  18. Individual variation in p53 and Cip1 expression profiles in normal human fibroblast strains following exposure to high-let radiation

    Energy Technology Data Exchange (ETDEWEB)

    Carpenter, T.R.; Johnson, N.F.; Gilliland, F.D. [Univ. of New Mexico, Albuquerque, NM (United States)] [and others

    1995-12-01

    Exposure to {alpha}-particles emitted by radon progeny appears to be the second-leading cause of lung cancer mortality. However, individual susceptibility to the carcinogenic effects of {alpha}-particles remains poorly characterized. Variation in susceptibility to cancer produced by certian classes of DNA-damaging chemicals is suspected to involve differences in metabolic activation and detoxication. Susceptibility to {alpha}-particle-induced cancer may involve variations in capacity or opportunity to repair DNA damage. Subtle variations in DNA repair capacity would more likely explain radon-related lung cancer susceptibility. The p53 tumor suppressor protein accumulates as a cellular response to DNA damage from ionizing radiation and regulates arrest in the G{sub 1} portion of the cell cycle. Arrest in G{sub 1} portion of the cell cycle. While upstream regulation of p53 protein stability is poorly understood, variations in the ability to accumulate p53 following DNA damage represent potential variations in lung cancer susceptibility related to radon progeny. Further, transcription of the cell-cycle regulatory gene Cip1 is regulated by p53 and increases following ionizing radiation. Therefore, variations in the expression of Cip1 following {alpha}-particle exposure may also be a susceptibility factor in radon-related lung cancers. The purpose of the present investigation was to measure p53 and Cip1 protein induction following {alpha}-particle exposure of fibroblast lines from nine individuals to determine if there were significant variations. The expression of Cip1 protein indicates the differences in response are biologically relevant.

  19. Suppression of c-Myc enhances p21(WAF1/CIP1) -mediated G1 cell cycle arrest through the modulation of ERK phosphorylation by ascochlorin.

    Science.gov (United States)

    Jeong, Yun-Jeong; Hoe, Hyang-Sook; Cho, Hyun-Ji; Park, Kwan-Kyu; Kim, Dae-Dong; Kim, Cheorl-Ho; Magae, Junji; Kang, Dong Wook; Lee, Sang-Rae; Chang, Young-Chae

    2017-08-18

    Numerous anti-cancer agents inhibit cell cycle progression via a p53-dependent mechanism; however, other genes such as the proto-oncogene c-Myc are promising targets for anticancer therapy. In the present study, we provide evidence that ascochlorin, an isoprenoid antibiotic, is a non-toxic anti-cancer agent that induces G1 cell cycle arrest and p21(WAF1/CIP1) expression by downregulating of c-Myc protein expression. Ascochlorin promoted the G1 arrest, upregulated p53 and p21(WAF1/CIP1) , and downregulated c-Myc in HCT116 cells. In p53-deficient cells, ascochlorin enhanced the expression of G1 arrest-related genes except p53. Small interfering RNA (siRNA) mediated c-Myc silencing indicated that the transcriptional repression of c-Myc was related to ascochlorin-mediated modulation of p21(WAF1/CIP1) expression. Ascochlorin suppressed the stabilization of the c-Myc protein by inhibiting ERK and P70S6K/4EBP1 phosphorylation, whereas it had no effect on c-Myc degradation mediated by PI3K/Akt/GSK3β. The ERK inhibitor PD98059 and siRNA-mediated ERK silencing induced G1 arrest and p21(WAF1/CIP1) expression by downregulating c-Myc in p53-deficient cells. These results indicated that ascochlorin-induced G1 arrest is associated with the repression of ERK phosphorylation and c-Myc expression. Thus, we reveal a role for ascochlorin in inhibiting tumor growth via G1 arrest, and identify a novel regulatory mechanism for ERK /c-Myc. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

  20. Immunohistochemical examination of the INK4 and Cip inhibitors in the rat neonatal cerebellum: cellular localization and the impact of protein calorie malnutrition.

    Science.gov (United States)

    Shambaugh, G E; Haines, G K; Koch, A; Lee, E J; Zhou, J n; Pestell, R

    2000-02-01

    Expression of the cyclin-dependent kinase inhibitors (CKIs) has been linked to the inhibition of cellular proliferation and the induction of differentiation. Based on structure function analysis, two distinct families of CDKIs, the INK4 and the Cip/Kip family have been identified. The INK4 family member p16(Ink4), and the Cip/Kip protein p27(Kip1) have been implicated in normal development of the CNS and cerebellum. Recent studies have suggested a functional inter-dependence between the CKI and the abundance of cyclin D1 in orchestrating growth factor-induced cellular proliferation. The neonatal rat cerebellum undergoes proliferative growth and differentiation, localized to distinct topographical regions and cell types. The cell type and the temporal profile of CKI expression during postnatal cerebellar development had not been described. The current studies determined the specific cerebellar cell types in which the CKIs were expressed during post natal development by co-staining for cell-type specific markers. p16(Ink4a) and p27(Kip1) immunostaining was identified in both neurons and glial cells, increasing progressively between postnatal days 6 to 13 into adulthood. By contrast, neuronal and glial cell p21(Cip1) staining was prominent at days 6-11 and decreased thereafter. Cyclin D1 was expressed in the proliferating external granular cells, with occassional staining in the molecular, and internal granular layers. Dual immunostaining demonstrated cyclin D1 within cells expressing CKI (p16(Ink4a), p21(Cip1),p27(Kip1)). Cerebellar cellular growth arrest, induced by protein-calorie malnutrition, inhibited cyclin D1 protein levels without affecting CKI immunostaining suggesting CKI do not mediate the developmental arrest. These results demonstrate that the CKIs are induced by differentiation cues in specific cell types with distinct kinetics in the developing cerebellum in vivo.

  1. Deficiency of cyclin-dependent kinase inhibitors p21{sup Cip1} and p27{sup Kip1} accelerates atherogenesis in apolipoprotein E-deficient mice

    Energy Technology Data Exchange (ETDEWEB)

    Akyuerek, Levent M. [National Human Genome Research Institute and National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892 (United States); Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology, University of Gothenburg, Goeteborg, SE-405 30 (Sweden); Boehm, Manfred; Olive, Michelle [National Human Genome Research Institute and National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892 (United States); Zhou, Alex-Xianghua [Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology, University of Gothenburg, Goeteborg, SE-405 30 (Sweden); San, Hong [National Human Genome Research Institute and National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892 (United States); Nabel, Elizabeth G., E-mail: enabel@partners.org [National Human Genome Research Institute and National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892 (United States)

    2010-05-28

    Cyclin-dependent kinase inhibitors, p21{sup Cip1} and p27{sup Kip1}, are upregulated during vascular cell proliferation and negatively regulate growth of vascular cells. We hypothesized that absence of either p21{sup Cip1} or p27{sup Kip1} in apolipoprotein E (apoE)-deficiency may increase atherosclerotic plaque formation. Compared to apoE{sup -/-} aortae, both apoE{sup -/-}/p21{sup -/-} and apoE{sup -/-}/p27{sup -/-} aortae exhibited significantly more atherosclerotic plaque following a high-cholesterol regimen. This increase was particularly observed in the abdominal aortic regions. Deficiency of p27{sup Kip1} accelerated plaque formation significantly more than p21{sup -/-} in apoE{sup -/-} mice. This increased plaque formation was in parallel with increased intima/media area ratios. Deficiency of p21{sup Cip1} and p27{sup Kip1} accelerates atherogenesis in apoE{sup -/-} mice. These findings have significant implications for our understanding of the molecular basis of atherosclerosis associated with excessive proliferation of vascular cells.

  2. CASL L1 Milestone report : CASL.P4.01, sensitivity and uncertainty analysis for CIPS with VIPRE-W and BOA.

    Energy Technology Data Exchange (ETDEWEB)

    Sung, Yixing (Westinghouse Electric Company LLC, Cranberry Township, PA); Adams, Brian M.; Secker, Jeffrey R. (Westinghouse Electric Company LLC, Cranberry Township, PA)

    2011-12-01

    The CASL Level 1 Milestone CASL.P4.01, successfully completed in December 2011, aimed to 'conduct, using methodologies integrated into VERA, a detailed sensitivity analysis and uncertainty quantification of a crud-relevant problem with baseline VERA capabilities (ANC/VIPRE-W/BOA).' The VUQ focus area led this effort, in partnership with AMA, and with support from VRI. DAKOTA was coupled to existing VIPRE-W thermal-hydraulics and BOA crud/boron deposit simulations representing a pressurized water reactor (PWR) that previously experienced crud-induced power shift (CIPS). This work supports understanding of CIPS by exploring the sensitivity and uncertainty in BOA outputs with respect to uncertain operating and model parameters. This report summarizes work coupling the software tools, characterizing uncertainties, and analyzing the results of iterative sensitivity and uncertainty studies. These studies focused on sensitivity and uncertainty of CIPS indicators calculated by the current version of the BOA code used in the industry. Challenges with this kind of analysis are identified to inform follow-on research goals and VERA development targeting crud-related challenge problems.

  3. Cholesterol Retards Senescence in Bone Marrow Mesenchymal Stem Cells by Modulating Autophagy and ROS/p53/p21Cip1/Waf1 Pathway

    Directory of Open Access Journals (Sweden)

    Mingyu Zhang

    2016-01-01

    Full Text Available In the present study, we demonstrated that bone marrow mesenchymal stem cells (BMSCs of the 3rd passage displayed the senescence-associated phenotypes characterized with increased activity of SA-β-gal, altered autophagy, and increased G1 cell cycle arrest, ROS production, and expression of p53 and p21Cip1/Waf1 compared with BMSCs of the 1st passage. Cholesterol (CH reduced the number of SA-β-gal positive cells in a dose-dependent manner in aging BMSCs induced by H2O2 and the 3rd passage BMSCs. Moreover, CH inhibited the production of ROS and expression of p53 and p21Cip1/Waf1 in both cellular senescence models and decreased the percentage of BMSCs in G1 cell cycle in the 3rd passage BMSCs. CH prevented the increase in SA-β-gal positive cells induced by RITA (reactivation of p53 and induction of tumor cell apoptosis, a p53 activator or 3-MA (3-methyladenine, an autophagy inhibitor. Our results indicate that CH not only is a structural component of cell membrane but also functionally contributes to regulating cellular senescence by modulating cell cycle, autophagy, and the ROS/p53/p21Cip1/Waf1 signaling pathway.

  4. Oridonin inhibits gefitinib-resistant lung cancer cells by suppressing EGFR/ERK/MMP-12 and CIP2A/Akt signaling pathways.

    Science.gov (United States)

    Xiao, Xiangling; He, Zhongwei; Cao, Wei; Cai, Fen; Zhang, Liang; Huang, Qiuyue; Fan, Chunsheng; Duan, Chao; Wang, Xiaobo; Wang, Jiu; Liu, Ying

    2016-06-01

    Oridonin (Ori), a diterpenoid compound extracted from traditional medicinal herbs, elicits antitumor effects on many cancer types. However, whether Ori can be used in gefitinib-resistant non-small cell lung cancer (NSCLC) cells remains unclear. This study investigated the antitumor activity and underlying mechanisms of Ori. Results demonstrated that this compound dose-dependently inhibited the proliferation, invasion, and migration of the gefitinib-resistant NSCLC cells in vitro. Ori also significantly downregulated the phosphorylation of EGFR, ERK, Akt, expression levels of matrix metalloproteinase-12 (MMP-12), and the cancerous inhibitor of protein phosphatase 2A (CIP2A). In addition, Ori upregulated protein phosphatase 2A (PP2A) activity of gefitinib-resistant NSCLC cells. Ori combined with docetaxel synergistically inhibited these cells. Ori also inhibited tumor growth in murine models. Immunohistochemistry results further revealed that Ori downregulated phospho-EGFR, MMP-12, and CIP2A in vivo. These findings indicated that Ori can inhibit the proliferation, invasion, and migration of gefitinib-resistant NSCLC cells by suppressing EGFR/ERK/MMP-12 and CIP2A/PP2A/Akt signaling pathways. Thus, Ori may be a novel effective candidate to treat gefitinib-resistant NSCLC.

  5. Glycogen synthase kinase 3beta phosphorylates p21WAF1/CIP1 for proteasomal degradation after UV irradiation.

    Science.gov (United States)

    Lee, Ji Young; Yu, Su Jin; Park, Yun Gyu; Kim, Joon; Sohn, Jeongwon

    2007-04-01

    UV irradiation has been reported to induce p21(WAF1/CIP1) protein degradation through a ubiquitin-proteasome pathway, but the underlying biochemical mechanism remains to be elucidated. Here, we show that ser-114 phosphorylation of p21 protein by glycogen synthase kinase 3beta (GSK-3beta) is required for its degradation in response to UV irradiation and that GSK-3beta activation is a downstream event in the ATR signaling pathway triggered by UV. UV transiently increased GSK-3beta activity, and this increase could be blocked by caffeine or by ATR small interfering RNA, indicating ATR-dependent activation of GSK-3beta. ser-114, located within the putative GSK-3beta target sequence, was phosphorylated by GSK-3beta upon UV exposure. The nonphosphorylatable S114A mutant of p21 was protected from UV-induced destabilization. Degradation of p21 protein by UV irradiation was independent of p53 status and prevented by proteasome inhibitors. In contrast to the previous report, the proteasomal degradation of p21 appeared to be ubiquitination independent. These data show that GSK-3beta is activated by UV irradiation through the ATR signaling pathway and phosphorylates p21 at ser-114 for its degradation by the proteasome. To our knowledge, this is the first demonstration of GSK-3beta as the missing link between UV-induced ATR activation and p21 degradation.

  6. ACS algorithm-based adaptive fuzzy PID controller and its application to CIP-Ⅰ intelligent leg

    Institute of Scientific and Technical Information of China (English)

    TAN Guan-zheng; DOU Hong-quan

    2007-01-01

    Based on the ant colony system (ACS) algorithm and fuzzy logic control, a new design method for optimal fuzzy PID controller was proposed. In this method, the ACS algorithm was used to optimize the input/output scaling factors of fuzzy PID controller to generate the optimal fuzzy control rules and optimal real-time control action on a given controlled object. The designed controller, called the Fuzzy-ACS PID controller, was used to control the CIP-Ⅰ intelligent leg. The simulation experiments demonstrate that this controller has good control performance. Compared with other three optimal PID controllers designed respectively by using the differential evolution algorithm, the real-coded genetic algorithm, and the simulated annealing, it was verified that the Fuzzy-ACS PID controller has better control performance. Furthermore, the simulation results also verify that the proposed ACS algorithm has quick convergence speed, small solution variation, good dynamic convergence behavior, and high computation efficiency in searching for the optimal input/output scaling factors.

  7. Doxorubicin Activates Hepatitis B Virus Replication by Elevation of p21 (Waf1/Cip1 and C/EBPα Expression.

    Directory of Open Access Journals (Sweden)

    Yu-Fang Chen

    Full Text Available Hepatitis B virus reactivation is an important medical issue in cancer patients who undergo systemic chemotherapy. Up to half of CHB carriers receiving chemotherapy develop hepatitis and among these cases a notable proportion are associated with HBV reactivation. However, the molecular mechanism(s through which various chemotherapeutic agents induce HBV reactivation is not yet fully understood. In this study, we investigated the role of the cell cycle regulator p21 (Waf1/Cip1 in the modulation of HBV replication when a common chemotherapeutic agent, doxorubicin, is present. We showed that p21 expression was increased by doxorubicin treatment. This elevation in p21 expression enhanced the expression of CCAAT/enhancer-binding protein α (C/EBPα; such an increase is likely to promote the binding of C/EBPα to the HBV promoter, which will contribute to the activation of HBV replication. Our current study thus reveals the mechanism underlying doxorubicin modulation of HBV replication and provides an increased understanding of HBV reactivation in CHB patients who are receiving systemic chemotherapy.

  8. Optimization of isonovalal production from alpha-pinene oxide using permeabilized cells of Pseudomonas rhodesiae CIP 107491.

    Science.gov (United States)

    Fontanille, P; Larroche, C

    2003-01-01

    Optimization studies on the synthesis of isonovalal from alpha-pinene oxide by Pseudomonas rhodesiae CIP 107491 operated in a biphasic medium are presented. Three key parameters are identified. The first is the need for a permeabilization of cells by freezing them and then treating the thawed material with an organic solvent such as chloroform, toluene or diethyl ether. This operation allows both enzyme release into the aqueous phase outside the cells and an improvement in the transport properties of both substrate and product across the cell membrane, strongly increasing reaction rates. The second is that the enzyme alpha-pinene oxide lyase, which exhibits an irreversible inactivation by isonovalal (or a by-product), presents a constant turn-over, i.e., the total product synthesis is proportional to the biomass loading and is close to 108 mmol (16.4 g) isonovalal l(-1) g(-1) biomass. The third phenomenon is that the biphasic system used is not phase-transfer-limited, a feature attributed to the spontaneous formation of an oil-in-water emulsion. It is thus possible to carry out a very efficient process, allowing the recovery of 2.63 mol isonovalal l(-1) (400 g l(-1)) from 25 g biomass l(-1) in 2.5 h, corresponding to an average reaction rate as high as 0.70 mmol min(-1) g(-1) cells (160 g l(-1) h(-1)).

  9. Production of trans-2-methyl-5-isopropylhexa-2,5-dienoic acid by Pseudomonas rhodesiae CIP 107491.

    Science.gov (United States)

    Linares, Denis; Martinez, Daniela; Fontanille, Pierre; Larroche, Christian

    2008-07-01

    The feasibility of trans-2-methyl-5-isopropylhexa-2,5-dienoic acid (novalic acid) accumulation using the alpha-pinene degradation pathway of Pseudomonas rhodesiae CIP 107491 was studied. This appeared possible by using concentrated living bacterial cells produced under oxygen limitation with alpha-pinene as sole carbon source. The second step of the process, the bioconversion itself, had to be performed without oxygen limitation due to the need for cofactor regeneration. Results showed that a not yet reported cofactor-dependent enzymatic isomerization of isonovalal into novalal was likely to occur and that both aldehyde isomers could be oxidized to the corresponding acid. Precursors tested, alpha-pinene oxide and isonovalal had a strong permeabilization effect on bacterial cells. This effect, which increased from the oxide to the aldehyde, led to an inactivation of the respiratory chain and to acids synthesis stop. Present results allowed to obtain about 12 g/L acids (80% novalic acid) with an average yield close to 50% after 12h reaction in a biphasic system using alpha-pinene oxide as precursor .

  10. Estruturas secretoras em cipó-d'alho (Mansoa standleyi (Steyerm. A. H. Gentry, Bignoniaceae: ocorrência e morfologia Secretory structures in cipó-d'alho (Mansoa standleyi (Steyerm. A. H. Gentry, Bignoniaceae: occurrence and morphology

    Directory of Open Access Journals (Sweden)

    Raimunda Conceição Vilhena-Potiguara

    2012-09-01

    Full Text Available Espécies de Mansoa são denominadas de "cipó-d'alho", por exalarem odor de alho das partes vegetativas e reprodutivas. Contudo, dados sobre morfologia e distribuição das estruturas secretoras presentes em Mansoa são escassos e ausentes para M. standleyi. O presente trabalho objetivou caracterizar a ocorrência e morfologia das estruturas secretoras do eixo vegetativo aéreo de M. standleyi. Para tanto, amostras da lâmina foliolar e de regiões nodais foram fixadas e submetidas às técnicas histológicas e de microscopia eletrônica de varredura. Testes histoquímicos, com os respectivos controles foram aplicados nas estruturas secretoras em fase secretora. Indivíduos de formigas e moscas, que visitavam a espécie foram amostrados, preservados e identificados por entomólogo. As estruturas secretoras do eixo vegetativo aéreo de M. standleyi estão representadas por tricomas glandulares dos tipos pateliformes e peltados. Todos com desenvolvimento assincrônico e presentes nas regiões nodais e lâmina foliolar, principalmente nas partes mais jovens. Nas regiões nodais, os tricomas formam um complexo secretor e, na lâmina foliolar, estão dispersos. As análises histoquímicas revelaram que os tricomas pateliformes são de fato nectários extraflorais e que os tricomas peltados, apresentam uma fração de alcaloides. Os visitantes das glândulas nodais correspondem a formigas Crematogaster (Formicidae e Ectatomma brunea (Vespoidea, Formicidae e moscas Oxysarcodexia (Sarcophagidae, subfamília Utitidae (Ulidiidae. Mansoa standleyi possui as estruturas secretoras do eixo vegetativo aéreo semelhantes às citadas pela literatura para Bignoniaceae, sendo esta a primeira vez, que um nectário extrafloral é descrito para a espécie.Species of Mansoa are called "cipó-d'alho" because of the smell of garlic that wafts from their vegetative and reproductive parts. Since data on the morphology and occurrence of their secretory structures are

  11. DDX3, a DEAD box RNA helicase with tumor growth-suppressive property and transcriptional regulation activity of the p21waf1/cip1 promoter, is a candidate tumor suppressor.

    Science.gov (United States)

    Chao, Chi-Hong; Chen, Chun-Ming; Cheng, Pei-Lin; Shih, Jing-Wen; Tsou, Ann-Ping; Lee, Yan-Hwa Wu

    2006-07-01

    DDX3 is a DEAD box RNA helicase with diverse biological functions. Using colony formation assay, our results revealed that DDX3 inhibited the colony formation ability of various tumor cells, and this inhibition might be due to a reduced growth rate caused by DDX3. Additionally, we identified p21(waf1/cip1), a cyclin-dependent kinase inhibitor, as a target gene of DDX3, and the up-regulation of p21(waf1/cip1) expression accounted for the colony-suppressing activity of DDX3. Moreover, DDX3 exerted its transactivation function on p21(waf1/cip1) promoter through an ATPase-dependent but helicase-independent mechanism, and the four Sp1 sites located within the -123 to -63 region, relative to the transcription start site of p21(waf1/cip1) promoter, were essential for the response to DDX3. Furthermore, DDX3 interacted and cooperated with Sp1 to up-regulate the promoter activity of p21(waf1/cip1). To determine the relevance of DDX3 in clinical cancers, the expression profile of DDX3 in various tumors was also examined. A declined expression of DDX3 mRNA and protein was found in approximately 58% to 73% of hepatoma specimens, which led to the reduction of p21(waf1/cip1) expression in a manner independent of p53 status. Additionally, an alteration of subcellular localization from nuclei to cytoplasm was also observed in >70% of cutaneous squamous cell carcinoma samples. Because DDX3 exhibits tumor suppressor functions, such as a growth-suppressive property and transcriptional activation of the p21(waf1/cip1) promoter, and is inactivated through down-regulation of gene expression or alteration of subcellular localization in tumor cells, all these features together suggest that DDX3 might be a candidate tumor suppressor.

  12. Continuity theory

    CERN Document Server

    Nel, Louis

    2016-01-01

    This book presents a detailed, self-contained theory of continuous mappings. It is mainly addressed to students who have already studied these mappings in the setting of metric spaces, as well as multidimensional differential calculus. The needed background facts about sets, metric spaces and linear algebra are developed in detail, so as to provide a seamless transition between students' previous studies and new material. In view of its many novel features, this book will be of interest also to mature readers who have studied continuous mappings from the subject's classical texts and wish to become acquainted with a new approach. The theory of continuous mappings serves as infrastructure for more specialized mathematical theories like differential equations, integral equations, operator theory, dynamical systems, global analysis, topological groups, topological rings and many more. In light of the centrality of the topic, a book of this kind fits a variety of applications, especially those that contribute to ...

  13. Diversity assessment of benthic macroinvertebrates, yeasts, and microbiological indicators along a longitudinal gradient in Serra do Cipó, Brazil

    Directory of Open Access Journals (Sweden)

    M. Callisto

    Full Text Available The main goals of this study were: 1 to evaluate the structure, diversity, and functional trophic group composition of benthic macroinvertebrate communities; 2 to characterize water quality in the headwaters of the Doce river watershed, based on physical, chemical, and biological parameters (benthic macroinvertebrates, fecal coliforms, heterotrophic bacteria, and yeasts; and 3 to contribute to the knowledge of the structure and function of longitudinal gradients in lotic ecosystems in Brazil. A total of 60 benthic macroinvertebrate taxa were identified, the dominant group being the aquatic insects, with 50 families distributed in 8 orders. The dry period presented higher values of taxonomic richness and total density of benthic macroinvertebrates. A decreasing gradient was observed in these variable values from the 3rd order stretch down to the 6th order stretch. The highest Shannon-Wiener diversity values were found in the rainy period in the 3rd order stretches, which presented well-developed riparian forest. Besides the 3rd order stretches, the Pielou evenness index values were also high in the 6th order stretch. The collectors, together with the scrapers, predominated in the benthic macroinvertebrate communities in all river stretches, except in the 2nd, 4th, and 5th order stretches in the rainy period, where communities were dominated by filterers. The shredders and predators presented low densities for all river stretches. All microbiological variables presented low levels. Due to the high counts of heterotrophic bacteria and coliforms, the studied river stretches presented inadequate potability but adequate balneability levels. The results suggest that the structure, diversity, and composition of the benthic macroinvertebrate communities are influenced by the trophic resource availability, seasonality, and sediment heterogeneity. The microbiological results of this study allow inferring that the waters from Serra do Cipó have excellent

  14. p21{sup WAF1/CIP1} deficiency induces mitochondrial dysfunction in HCT116 colon cancer cells

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Ae Jeong; Jee, Hye Jin; Song, Naree; Kim, Minjee [Department of Biochemistry, College of Medicine, Dong-A University, Busan (Korea, Republic of); Mitochondria Hub Regulation Center, College of Medicine, Dong-A University, Busan (Korea, Republic of); Jeong, Seon-Young [Mitochondria Hub Regulation Center, College of Medicine, Dong-A University, Busan (Korea, Republic of); Department of Medical Genetics, Ajou University School of Medicine (Korea, Republic of); Yun, Jeanho, E-mail: yunj@dau.ac.kr [Department of Biochemistry, College of Medicine, Dong-A University, Busan (Korea, Republic of); Mitochondria Hub Regulation Center, College of Medicine, Dong-A University, Busan (Korea, Republic of)

    2013-01-11

    Highlights: Black-Right-Pointing-Pointer p21{sup -/-} HCT116 cells exhibited an increase in mitochondrial mass. Black-Right-Pointing-Pointer The expression levels of PGC-1{alpha} and AMPK were upregulated in p21{sup -/-} HCT116 cells. Black-Right-Pointing-Pointer The proliferation of p21{sup -/-} HCT116 cells in galactose medium was significantly impaired. Black-Right-Pointing-Pointer p21 may play a role in maintaining proper mitochondrial mass and respiratory function. -- Abstract: p21{sup WAF1/CIP1} is a critical regulator of cell cycle progression. However, the role of p21 in mitochondrial function remains poorly understood. In this study, we examined the effect of p21 deficiency on mitochondrial function in HCT116 human colon cancer cells. We found that there was a significant increase in the mitochondrial mass of p21{sup -/-} HCT116 cells, as measured by 10-N-nonyl-acridine orange staining, as well as an increase in the mitochondrial DNA content. In contrast, p53{sup -/-} cells had a mitochondrial mass comparable to that of wild-type HCT116 cells. In addition, the expression levels of the mitochondrial biogenesis regulators PGC-1{alpha} and TFAM and AMPK activity were also elevated in p21{sup -/-} cells, indicating that p21 deficiency induces the rate of mitochondrial biogenesis through the AMPK-PGC-1{alpha} axis. However, the increase in mitochondrial biogenesis in p21{sup -/-} cells did not accompany an increase in the cellular steady-state level of ATP. Furthermore, p21{sup -/-} cells exhibited significant proliferation impairment in galactose medium, suggesting that p21 deficiency induces a defect in the mitochondrial respiratory chain in HCT116 cells. Taken together, our results suggest that the loss of p21 results in an aberrant increase in the mitochondrial mass and in mitochondrial dysfunction in HCT116 cells, indicating that p21 is required to maintain proper mitochondrial mass and respiratory function.

  15. p21{sup WAF1/Cip1/Sdi1} knockout mice respond to doxorubicin with reduced cardiotoxicity

    Energy Technology Data Exchange (ETDEWEB)

    Terrand, Jerome; Xu, Beibei; Morrissy, Steve; Dinh, Thai Nho [Department of Pharmacology,College of Medicine, University of Arizona, 1501 N. Campbell Ave, Tucson, AZ 85724 (United States); Williams, Stuart [Biomedical Engineering Program, College of Medicine, University of Arizona, 1501 N. Campbell Ave, Tucson, AZ 85724 (United States); Chen, Qin M., E-mail: qchen@email.arizona.edu [Department of Pharmacology,College of Medicine, University of Arizona, 1501 N. Campbell Ave, Tucson, AZ 85724 (United States)

    2011-11-15

    Doxorubicin (Dox) is an antineoplastic agent that can cause cardiomyopathy in humans and experimental animals. As an inducer of reactive oxygen species and a DNA damaging agent, Dox causes elevated expression of p21{sup WAF1/Cip1/Sdi1} (p21) gene. Elevated levels of p21 mRNA and p21 protein have been detected in the myocardium of mice following Dox treatment. With chronic treatment of Dox, wild type (WT) animals develop cardiomyopathy evidenced by elongated nuclei, mitochondrial swelling, myofilamental disarray, reduced cardiac output, reduced ejection fraction, reduced left ventricular contractility, and elevated expression of ANF gene. In contrast, p21 knockout (p21KO) mice did not show significant changes in the same parameters in response to Dox treatment. In an effort to understand the mechanism of the resistance against Dox induced cardiomyopathy, we measured levels of antioxidant enzymes and found that p21KO mice did not contain elevated basal or inducible levels of glutathione peroxidase and catalase. Measurements of 6 circulating cytokines indicated elevation of IL-6, IL-12, IFN{gamma} and TNF{alpha} in Dox treated WT mice but not p21KO mice. Dox induced elevation of IL-6 mRNA was detected in the myocardium of WT mice but not p21KO mice. While the mechanism of the resistance against Dox induced cardiomyopathy remains unclear, lack of inflammatory response may contribute to the observed cardiac protection in p21KO mice. -- Highlights: Black-Right-Pointing-Pointer Doxorubicin induces p21 elevation in the myocardium. Black-Right-Pointing-Pointer Doxorubicin causes dilated cardiomyopathy in wild type mice. Black-Right-Pointing-Pointer p21 Knockout mice are resistant against doxorubicin induced cardiomyopathy. Black-Right-Pointing-Pointer Lack of inflammatory response correlates with the resistance in p21 knockout mice.

  16. Overexpression of caspase 7 is ERα dependent to affect proliferation and cell growth in breast cancer cells by targeting p21(Cip).

    Science.gov (United States)

    Chaudhary, S; Madhukrishna, B; Adhya, A K; Keshari, S; Mishra, S K

    2016-04-18

    Caspase 7 (CASP7) expression has important function during cell cycle progression and cell growth in certain cancer cells and is also involved in the development and differentiation of dental tissues. However, the function of CASP7 in breast cancer cells is unclear. The aim of this study was to analyze the expression of CASP7 in breast carcinoma patients and determine the role of CASP7 in regulating tumorigenicity in breast cancer cells. In this study, we show that the CASP7 expression is high in breast carcinoma tissues compared with normal counterpart. The ectopic expression of CASP7 is significantly associated with ERα expression status and persistently elevated in different stages of the breast tumor grades. High level of CASP7 expression showed better prognosis in breast cancer patients with systemic endocrine therapy as observed from Kaplan-Meier analysis. S3 and S4, estrogen responsive element (ERE) in the CASP7 promoter, is important for estrogen-ERα-mediated CASP7 overexpression. Increased recruitment of p300, acetylated H3 and pol II in the ERE region of CASP7 promoter is observed after hormone stimulation. Ectopic expression of CASP7 in breast cancer cells results in cell growth and proliferation inhibition via p21(Cip) reduction, whereas small interfering RNA (siRNA) mediated reduction of CASP7 rescued p21(Cip) levels. We also show that pro- and active forms of CASP7 is located in the nucleus apart from cytoplasmic region of breast cancer cells. The proliferation and growth of breast cancer cells is significantly reduced by broad-spectrum peptide inhibitors and siRNA of CASP7. Taken together, our findings show that CASP7 is aberrantly expressed in breast cancer and contributes to cell growth and proliferation by downregulating p21(Cip) protein, suggesting that targeting CASP7-positive breast cancer could be one of the potential therapeutic strategies.

  17. Continuation calculus

    Directory of Open Access Journals (Sweden)

    Bram Geron

    2013-09-01

    Full Text Available Programs with control are usually modeled using lambda calculus extended with control operators. Instead of modifying lambda calculus, we consider a different model of computation. We introduce continuation calculus, or CC, a deterministic model of computation that is evaluated using only head reduction, and argue that it is suitable for modeling programs with control. It is demonstrated how to define programs, specify them, and prove them correct. This is shown in detail by presenting in CC a list multiplication program that prematurely returns when it encounters a zero. The correctness proof includes termination of the program. In continuation calculus we can model both call-by-name and call-by-value. In addition, call-by-name functions can be applied to call-by-value results, and conversely.

  18. Reingeniería del sistema de limpieza y sanitización por el método cip para las envasadoras de bebidas gaseosas

    OpenAIRE

    2008-01-01

    Los procedimientos de limpieza en las envasadoras, generalmente utilizan un sistema de limpieza CIP con dos pasos de agua caliente a 80ºC. (176 ºF.), los cuales han sido muy acertados en la obtención de estándares microbianos óptimos. Sin embargo, este procedimiento de saneamiento de la envasadora, que emplea vapor, causa desperdicio de tiempo y energía en forma excesiva y, además, produce un peligroso incremento de la temperatura al área de envasado. El trabajo de la presente Tesis demostrar...

  19. Platinum-(Ⅳ)-derivative satraplatin induced G2/M cell cycle perturbation via p53-p21waf1/cip1-independent pathway in human colorectal cancer cells

    Institute of Scientific and Technical Information of China (English)

    Murugan KALIMUTHO; Antonella MINUTOLO; Sandro GRELLI; Giorgio FEDERICI; Sergio BERNARDINI

    2011-01-01

    Aim:Platinum-(Ⅳ)-derivative satraplatin represents a new generation of orally available anti-cancer drugs that are under development for the treatment of several cancers.Understanding the mechanisms of cell cycle modulation and apoptosis is necessary to define the mode of action of satraplatin.In this study,we investigate the ability of satraplatin to induce cell cycle perturbation,clonogenicity loss and apoptosis in colorectal cancer (CRC) cells.Methods:CRC cells were treated with satraplatin,and the effects of satraplatin on apoptosis and the cell cycle were evaluated by flow cytometry.Western blot analysis was used to investigate the effects of satraplatin on cell cycle and apoptosis-related proteins.RTqPCR was used to evaluate p53-related mRNA modulation.Results:Satraplatin induced an accumulation of CRC cells predominantly in the G2/M phase.Increased p53 protein expression was observed in the p53 wild-type HCT116 and LoVo cells together with p21waf1/cip1 protein up-regulation.However,p21waf1/cip1 protein accumulation was not observed in the p53 mutant HCT15,HT29,and WiDr cells,even when p53 protein expression was compromised,suggesting that the cell cycle perturbation is p53-p21waf1/cip1 independent.Following a candidate approach,we found an elevated expression of 14-3-3o protein levels in CRC cells,which was independent of the status of p53,further supporting the role of satraplatin in the perturbation of the G2/M cell cycle phase.Moreover,satraplatin treatment induced apoptosis along with Bcl-2 protein down-regulation and abrogated the clonogenic formation of CRC cells in vitro.Conclusion:Collectively,our data suggest that satraplatin induces apoptosis in CRC cells,which is preceded by cell cycle arrest at G2/M due to the effect of 14-3-3σ and in a p53-p21waf1/cip1-independent manner.Taken together,these findings highlight the potential use of satraplatin for CRC treatment.

  20. Contaminação biológica e o uso de animais de montaria no Parque Nacional da Serra do Cipó - MG 

    OpenAIRE

    Fabiola Saporiti Angerami de Andrade

    2010-01-01

    O Parque Nacional da Serra do Cipó tem passado por um importante desafio de manejo que é a definição sobre o uso de animais de montaria por parte dos visitantes. Recentemente, o Governo Brasileiro lançou um documento intitulado Diretrizes para visitação em unidades de conservação o qual dita algumas normas para essa atividade de uso público. No entanto, há questões relevantes de incoerências deste com o Sistema Nacional de Unidades de Conservação. A presença dos animais numa unidade de conser...

  1. The complex perovskite-related superstructure Ba[sub 2]Fe[sub 2]O[sub 5] solved by HREM and CIP

    Energy Technology Data Exchange (ETDEWEB)

    Zou, X.D.; Hovmoeller, S. (Stockholm Univ. (Sweden). Dept. of Structural Chemistry); Parras, M.; Gonzalez-Calbet, J.M. (Univ. Complutense, Madrid (Spain). Dept. de Quimica Inorganica); Vallet-Regi, M. (Univ. Complutense, Madrid (Spain). Dept. de Quimica Inorganica y Bioinorganica); Grenier, J.C. (Bordeaux-1 Univ., 33 - Talence (France). Lab. de Chimie du Solide du CNRS)

    1993-01-01

    Barium ferrite Ba[sub 2]Fe[sub 2]O[sub 5] is a complex perovskite-related structure. The crystal is monoclinic P2[sub 1]/c, with a = 6.969(1), b = 11.724(1), c = 23.431(5) A, [beta] = 98.74(1)[sup 0]. The composition of one unit cell is Ba[sub 28]Fe[sub 28]O[sub 70]. The structure was solved using a combination of high-resolution electron microscopy (HREM), crystallographic image processing (CIP) and electron diffraction. The structure can be described as a perovskite with 14 oxygen positions unoccupied with respect to the ABO[sub 3] stoichiometry in the monoclinic unit cell. These positions can be identified from the potential map obtained by HREM and CIP. They form two linear groups. Seven of them lie on a line along [476][sub m] (corresponding to [110][sub c] of perovskite), while the seven other symmetry-related positions lie along [476][sub m] (corresponding to [101][sub c] of perovskite). (orig.).

  2. Phlebotomine sand fly fauna and leishmania infection in the vicinity of the Serra do Cipó National Park, a natural Brazilian heritage site.

    Science.gov (United States)

    Lana, Rosana Silva; Michalsky, Érika Monteiro; Fortes-Dias, Consuelo Latorre; França-Silva, João Carlos; Lara-Silva, Fabiana de Oliveira; Lima, Ana Cristina Vianna Mariano da Rocha; Moreira de Avelar, Daniel; Martins, Juliana Cristina Dias; Dias, Edelberto Santos

    2015-01-01

    In the New World, the leishmaniases are primarily transmitted to humans through the bites of Leishmania-infected Lutzomyia (Diptera: Psychodidae) phlebotomine sand flies. Any or both of two basic clinical forms of these diseases are endemic to several cities in Brazil--the American cutaneous leishmaniasis (ACL) and the American visceral leishmaniasis (AVL). The present study was conducted in the urban area of a small-sized Brazilian municipality (Jaboticatubas), in which three cases of AVL and nine of ACL have been reported in the last five years. Jaboticatubas is an important tourism hub, as it includes a major part of the Serra do Cipó National Park. Currently, no local data is available on the entomological fauna or circulating Leishmania. During the one-year period of this study, we captured 3,104 phlebotomine sand flies belonging to sixteen Lutzomyia species. In addition to identifying incriminated or suspected vectors of ACL with DNA of the etiological agent of AVL and vice versa, we also detected Leishmania DNA in unexpected Lutzomyia species. The expressive presence of vectors and natural Leishmania infection indicates favorable conditions for the spreading of leishmaniases in the vicinity of the Serra do Cipó National Park.

  3. Glucose-Induced Oxidative Stress Reduces Proliferation in Embryonic Stem Cells via FOXO3A/β-Catenin-Dependent Transcription of p21cip1

    Directory of Open Access Journals (Sweden)

    Darcie L. McClelland Descalzo

    2016-07-01

    Full Text Available Embryonic stem cells (ESCs, which are derived from a peri-implantation embryo, are routinely cultured in medium containing diabetic glucose (Glc concentrations. While pregnancy in women with pre-existing diabetes may result in small embryos, whether such high Glc levels affect ESC growth remains uncovered. We show here that long-term exposure of ESCs to diabetic Glc inhibits their proliferation, thereby mimicking in vivo findings. Molecularly, Glc exposure increased oxidative stress and activated Forkhead box O3a (FOXO3a, promoting increased expression and activity of the ROS-removal enzymes superoxide dismutase and catalase and the cell-cycle inhibitors p21cip1 and p27kip1. Diabetic Glc also promoted β-catenin nuclear localization and the formation of a complex with FOXO3a that localized to the promoters of Sod2, p21cip1, and potentially p27kip1. Our results demonstrate an adaptive response to increases in oxidative stress induced by diabetic Glc conditions that promote ROS removal, but also result in a decrease in proliferation.

  4. Phlebotomine Sand Fly Fauna and Leishmania Infection in the Vicinity of the Serra do Cipó National Park, a Natural Brazilian Heritage Site

    Directory of Open Access Journals (Sweden)

    Rosana Silva Lana

    2015-01-01

    Full Text Available In the New World, the leishmaniases are primarily transmitted to humans through the bites of Leishmania-infected Lutzomyia (Diptera: Psychodidae phlebotomine sand flies. Any or both of two basic clinical forms of these diseases are endemic to several cities in Brazil—the American cutaneous leishmaniasis (ACL and the American visceral leishmaniasis (AVL. The present study was conducted in the urban area of a small-sized Brazilian municipality (Jaboticatubas, in which three cases of AVL and nine of ACL have been reported in the last five years. Jaboticatubas is an important tourism hub, as it includes a major part of the Serra do Cipó National Park. Currently, no local data is available on the entomological fauna or circulating Leishmania. During the one-year period of this study, we captured 3,104 phlebotomine sand flies belonging to sixteen Lutzomyia species. In addition to identifying incriminated or suspected vectors of ACL with DNA of the etiological agent of AVL and vice versa, we also detected Leishmania DNA in unexpected Lutzomyia species. The expressive presence of vectors and natural Leishmania infection indicates favorable conditions for the spreading of leishmaniases in the vicinity of the Serra do Cipó National Park.

  5. Glucose-Induced Oxidative Stress Reduces Proliferation in Embryonic Stem Cells via FOXO3A/β-Catenin-Dependent Transcription of p21(cip1).

    Science.gov (United States)

    McClelland Descalzo, Darcie L; Satoorian, Tiffany S; Walker, Lauren M; Sparks, Nicole R L; Pulyanina, Polina Y; Zur Nieden, Nicole I

    2016-07-12

    Embryonic stem cells (ESCs), which are derived from a peri-implantation embryo, are routinely cultured in medium containing diabetic glucose (Glc) concentrations. While pregnancy in women with pre-existing diabetes may result in small embryos, whether such high Glc levels affect ESC growth remains uncovered. We show here that long-term exposure of ESCs to diabetic Glc inhibits their proliferation, thereby mimicking in vivo findings. Molecularly, Glc exposure increased oxidative stress and activated Forkhead box O3a (FOXO3a), promoting increased expression and activity of the ROS-removal enzymes superoxide dismutase and catalase and the cell-cycle inhibitors p21(cip1) and p27(kip1). Diabetic Glc also promoted β-catenin nuclear localization and the formation of a complex with FOXO3a that localized to the promoters of Sod2, p21(cip1), and potentially p27(kip1). Our results demonstrate an adaptive response to increases in oxidative stress induced by diabetic Glc conditions that promote ROS removal, but also result in a decrease in proliferation.

  6. Survey of the bryophytes of a gallery forest in the National Park of Serra do Cipó, Minas Gerais, Brazil

    Directory of Open Access Journals (Sweden)

    Ronaldo Viveiros de Sousa

    2015-03-01

    Full Text Available Serra do Cipó has attracted the interest of many researchers over the years because of its unique characteristics, particularly the fact that the site represents the transition between Cerrado and Atlantic Forest. The study area of Serra do Cipó is located along a gallery forest "córrego Três Pontinhas," at 19°16'00" S and 43°32'49" W and an altitude of 1,188 m. The objective of this study was to survey the Division Bryophyta in a gallery forest within the National Park of Serra do Cipo, Minas Gerais. Collections were made during the months of November 2009 and July 2011. We found 15 families, 26 genera, 43 species, and 4 varieties of mosses. The families with the largest number of species were Leucobryaceae (10, Sematophyllaceae (9, and Calymperaceae (6. Other families included Fissidentaceae (3, Bryaceae, Pylaisiadelphaceae, Pottiaceae, and Orthotrichaceae (2 spp. each; Brachytheciaceae, Cryphaeaceae, Fabroniaceae, Helicophyllaceae, Hypnaceae, Polytrichaceae, and Sphagnaceae had only 1 sp. each. Three new records for the state of Minas Gerais were found: Acroporium caespitosum, A. longirostre, and Colobodontium vulpinum.

  7. Exploração e manejo do cipó-titica (Heteropsis spp. / Exploitation and management of the vine Heteropsis spp.

    Directory of Open Access Journals (Sweden)

    Carlos César Durigan

    2012-04-01

    Full Text Available ResumoAs espécies hemiepífitas do gênero Heteropsis produzem raízes aéreas que são conhecidas na Amazônia brasileira como cipó-titica e titicão ou cipó timbó-açu, sendo fonte produtora de fibra de alto valor comercial, principalmente na fabricação de artesanatos e móveis. Essa matéria-prima é intensamente explorada de forma insustentável, tendo grande redução da disponibilidade desse recurso florestal, devido à diminuição ou extinção regional das plantas de cipó provocadas pelo inadequado manejo exploratório. Assim, alguns governos estaduais regulamentaram a conduta de manejo e exploração de cipós produtores de fibra, visando a sua manutenção e regeneração de suas raízes aéreas. Nesse sentido, o presente trabalho buscou, na literatura geral e científica, as informações sobre o marco legal da exploração e o plano de manejo, a ecologia das espécies, as etapas ligadas ao manejo florestal, inventário, intensidade e ciclo de corte bem como os procedimentos de coleta e beneficiamento dessas plantas, que são conhecimentos atualmente consolidados com normatização em poucos estados da Amazônia. Apesar disso, as áreas que foram intensamente exploradas se fazem necessário o desenvolvimento da produção sustentável com novos estudos, visando recuperação dessas populações por meio de técnicas de multiplicação vegetativa, plantio e nutrição vegetal, agregando-os ao manejo ecológico dos indivíduos remanescentes na floresta.AbstractThe hemiepiphytes species of the genus Heteropsis produces aerial roots that are known in the Brazilian Amazon as “cipó-titica and titicão or timbó-açu, and are a source of fiber of considerable commercial value used primarily to manufacture handicrafts and furniture. This raw material has been heavily exploited and mismanaged. As the consequent depletion and regional extinction of the plant vines has greatly reduced its availability likely a forest resource. This

  8. p21WAF1/CIP1在兔外伤性增生性玻璃体视网膜病变发生和发展中的抑制作用%Suppressing effect of p21WAF1/CIP1 on traumatic proliferative vitreoretinopathy in rabbits

    Institute of Scientific and Technical Information of China (English)

    袁志刚; 由彩云; 韩金栋; 李海燕; 颜华

    2016-01-01

    背景 p21是一种细胞周期蛋白依赖性激酶抑制剂,能阻止细胞从G1期进入S期,抑制细胞增生,研究认为内源性p21表达的动态变化可能与细胞增生性病变有关.外伤性增生性玻璃体视网膜病变(PVR)是眼部增生性反应相关性疾病,了解PVR过程中p21表达的动态变化可能为PVR的靶向治疗提供依据.目的 检测p21WAFl/CIP1在兔外伤性PVR中的动态变化,探讨其在外伤性PVR发病机制中的作用.方法 选取青紫蓝兔54只,采用随机数字表法将实验兔随机分为正常对照组(6只)和造模后7、14、21和28 d组(每组12只),每只兔任意选取一眼作为实验眼.各模型组兔眼玻璃体腔注射人富含血小板血浆(PRP)0.4 ml,同时于鼻上方角巩膜缘后5 mm处行巩膜外冷冻约5 s,以建立外伤性PVR模型.各组兔眼行眼部B型超声检查以评估建模情况.分别于造模后7、14、21和28 d以过量麻醉法处死实验兔并制备实验眼视网膜组织切片,采用苏木精-伊红染色法检测兔眼视网膜的形态表现,分别采用免疫组织化学染色、Western blot及逆转录PCR(RT-PCR)法检测兔视网膜中p21WAFl/CIP1蛋白及其mRNA的相对表达. 结果 正常对照组兔眼眼前后节均正常,模型组兔造模后1~7 d兔眼玻璃体中增生条索逐渐变粗,可见视网膜皱褶,造模后14d兔眼出现牵引性视网膜脱离,造模后28 d兔眼漏斗状视网膜脱离.视网膜病理组织学检查显示,造模后7d兔眼视网膜表面有增生膜和炎性细胞聚集,造模后28 d可见视网膜呈花瓣形固定皱褶,视网膜结构紊乱.免疫组织化学染色显示,p21WAF1/CIP1蛋白在正常对照组兔眼视网膜神经节细胞层及内核层的细胞核内呈强阳性表达,造模后7、14、21和28 d表达强度减弱,以造模后14d表达量最低.Western blot结果显示,正常对照组和造模后7、14、21和28 d组兔眼视网膜中p21WAF1/CIP1蛋白相对表达量分别为0.74±0.08、0.60

  9. Downregulation of LRRC8A protects human ovarian and alveolar carcinoma cells against Cisplatin-induced expression of p53, MDM2, p21Waf1/Cip1 and Caspase-9/-3 activation

    DEFF Research Database (Denmark)

    Sørensen, Belinda Halling; Nielsen, Dorthe; Thorsteinsdottir, Unnur Arna;

    2016-01-01

    ovarian (A2780) and alveolar (A549) carcinoma cells. In Cisplatin-sensitive cells Cisplatin treatment increases p53-protein level as well as downstream signaling, e.g., expression of p21(Waf1/Cip1), Bax, Noxa, MDM2, and activation of caspase-9/-3. In contrast, Cisplatin-resistant cells do not enter...

  10. Downregulation of LRRC8A protects human ovarian and alveolar carcinoma cells against Cisplatin-induced expression of p53, MDM2, p21Waf1/Cip1 and Caspase-9/-3 activation

    DEFF Research Database (Denmark)

    Sørensen, Belinda Halling; Nielsen, Dorthe; Thorsteinsdottir, Unnur Arna

    2016-01-01

    ovarian (A2780) and alveolar (A549) carcinoma cells. In Cisplatin-sensitive cells Cisplatin treatment increases p53-protein level as well as downstream signaling, e.g., expression of p21(Waf1/Cip1), Bax, Noxa, MDM2, and activation of caspase-9/-3. In contrast, Cisplatin-resistant cells do not enter...

  11. Hepatitis C virus core protein down-regulates p21(Waf1/Cip1 and inhibits curcumin-induced apoptosis through microRNA-345 targeting in human hepatoma cells.

    Directory of Open Access Journals (Sweden)

    Tzu-Yue Shiu

    Full Text Available BACKGROUND: Hepatitis C virus (HCV has been reported to regulate cellular microRNAs. The HCV core protein is considered to be a potential oncoprotein in HCV-related hepatocellular carcinoma, but HCV core-modulated cellular microRNAs are unknown. The HCV core protein regulates p21(Waf1/Cip1 expression. However, the mechanism of HCV core-associated p21(Waf1/Cip1 regulation remains to be further clarified. Therefore, we attempted to determine whether HCV core-modulated cellular microRNAs play an important role in regulating p21(Waf1/Cip1 expression in human hepatoma cells. METHODS: Cellular microRNA profiling was investigated in core-overexpressing hepatoma cells using TaqMan low density array. Array data were further confirmed by TaqMan real-time qPCR for single microRNA in core-overexpressing and full-length HCV replicon-expressing cells. The target gene of microRNA was examined by reporter assay. The gene expression was determined by real-time qPCR and Western blotting. Apoptosis was examined by annexin V-FITC apoptosis assay. Cell cycle analysis was performed by propidium iodide staining. Cell proliferation was analyzed by MTT assay. RESULTS: HCV core protein up- or down-regulated some cellular microRNAs in Huh7 cells. HCV core-induced microRNA-345 suppressed p21(Waf1/Cip1 gene expression through targeting its 3' untranslated region in human hepatoma cells. Moreover, the core protein inhibited curcumin-induced apoptosis through p21(Waf1/Cip1-targeting microRNA-345 in Huh7 cells. CONCLUSION AND SIGNIFICANCE: HCV core protein enhances the expression of microRNA-345 which then down-regulates p21(Waf1/Cip1 expression. It is the first time that HCV core protein has ever been shown to suppress p21(Waf1/Cip1 gene expression through miR-345 targeting.

  12. Transcriptional inhibition of p21{sup WAF1/CIP1} gene (CDKN1) expression by survivin is at least partially p53-dependent: Evidence for survivin acting as a transcription factor or co-factor

    Energy Technology Data Exchange (ETDEWEB)

    Tang, Lei [Department of Pharmacology and Therapeutics, Roswell Park Cancer Institute, Buffalo, NY (United States); Pre-Doctoral Chinese Fellowship Student, Second West China Hospital, Sichuan University, Sichuan (China); Ling, Xiang; Liu, Wensheng; Das, Gokul M. [Department of Pharmacology and Therapeutics, Roswell Park Cancer Institute, Buffalo, NY (United States); Li, Fengzhi, E-mail: fengzhi.li@roswellpark.org [Department of Pharmacology and Therapeutics, Roswell Park Cancer Institute, Buffalo, NY (United States)

    2012-05-04

    Highlights: Black-Right-Pointing-Pointer Survivin inhibits the expression of p21 protein, mRNA and promoter activity. Black-Right-Pointing-Pointer Survivin neutralizes p53-induced p21 expression and promoter activity. Black-Right-Pointing-Pointer Survivin physically interacts with p53 in cancer cells. Black-Right-Pointing-Pointer Genetic silencing of endogenous survivin upregulates p21 in p53 wild type cancer cells. Black-Right-Pointing-Pointer Both p53 and survivin interacts on the two p53-binding sites in the p21 promoter. -- Abstract: Growing evidence suggests a role for the antiapoptotic protein survivin in promotion of cancer cell G1/S transition and proliferation. However, the underlying mechanism is unclear. Further, although upregulation of p21{sup WAF1/CIP1} by p53 plays an important role in p53-mediated cell G1 arrests in response to various distresses, it is unknown whether survivin plays a role in the regulation of p21{sup WAF1/CIP1} expression. Here, we report that exogenous expression of survivin in p53-wild type MCF-7 breast cancer cells inhibits the expression of p21{sup WAF1/CIP1} protein, mRNA and promoter activity, while the survivin C84A mutant and antisense failed to do so. Cotransfection experiments in the p53 mutant H1650 lung cancer cell line showed that survivin neutralizes p53-induced p21{sup WAF1/CIP1} expression and promoter activity. Importantly, genetically silencing of endogenous survivin using lentiviral survivin shRNA also enhances endogenous p21 in p53 wild type cancer cells, suggesting the physiological relevance of the fining. We further demonstrated that both p53 and survivin interacts on the two p53-binding sites in the p21{sup WAF1/CIP1} promoter (-2313 to -2212; -1452 to -1310), and survivin physically interacts with p53 in cancer cells. Together, we propose that survivin may act as a transcription factor or cofactor to interact with p53 on the p21{sup WAF1/CIP1} promoter leading to the inhibition of p21{sup WAF1/CIP1

  13. Vibration Analysis of Continuous Maglev Guideway Considering the Magnetic Levitation System

    Institute of Scientific and Technical Information of China (English)

    TENG Yan-feng; TENG Nian-guan; KOU Xin-jian

    2008-01-01

    The dynamic interaction between moving vehicles and two-span continuous guideway was discussed. With the consideration of the magnetic levitation system, the maglev vehicle/guideway dynamic interaction model was developed. Numerical simulation was performed to understand dynamic characteristics of the guide- way used in practice. The results show that vehicle speed, span length and primary frequency of the guideway have an important influence on the dynamic responses of the guideway and there is no distinct trend towards resonance vibration when f1 equals 1.0. The definite way is to control the impact coefficient and acceleration of the guideway. The conclusions can serve the design of high speed maglev guideway.

  14. Isolation of a novel carotenoid, OH-chlorobactene glucoside hexadecanoate, and related rare carotenoids from Rhodococcus sp. CIP and their antioxidative activities.

    Science.gov (United States)

    Osawa, Ayako; Kasahara, Asami; Mastuoka, Shoko; Gassel, Sören; Sandmann, Gerhard; Shindo, Kazutoshi

    2011-01-01

    In the course of screening for antioxidative carotenoids from bacteria, we isolated and identified a novel carotenoid, OH-chlorobactene glucoside hexadecanoate (4), and rare carotenoids, OH-chlorobactene glucoside (1), OH-γ-carotene glucoside (2) and OH-4-keto-γ-carotene glucoside hexadecanoate (3) from Rhodococcus sp. CIP. The singlet oxygen ((1)O(2)) quenching model of these carotenoids showed potent antioxidative activities IC(50) 14.6 µM for OH-chlorobactene glucoside hexadecanoate (4), 6.5 µM for OH-chlorobactene glucoside (1), 9.9 µM for OH-γ-carotene glucoside (2) and 7.3 µM for OH-4-keto-γ-carotene glucoside hexadecanoate (3).

  15. Inhibition of MDA-MB-231 breast cancer cell proliferation and tumor growth by apigenin through induction of G2/M arrest and histone H3 acetylation-mediated p21(WAF1/CIP1) expression.

    Science.gov (United States)

    Tseng, Tsui-Hwa; Chien, Ming-Hsien; Lin, Wea-Lung; Wen, Yu-Ching; Chow, Jyh-Ming; Chen, Chi-Kuan; Kuo, Tsang-Chih; Lee, Wei-Jiunn

    2017-02-01

    Apigenin (4',5,7-trihydroxyflavone), a flavonoid commonly found in fruits and vegetables, has anticancer properties in various malignant cancer cells. However, the molecular basis of the anticancer effect remains to be elucidated. In this study, we investigated the cellular mechanisms underlying the induction of cell cycle arrest by apigenin. Our results showed that apigenin at the nonapoptotic induction concentration inhibited cell proliferation and induced cell cycle arrest at the G2/M phase in the MDA-MB-231 breast cancer cell line. Immunoblot analysis indicated that apigenin suppressed the expression of cyclin A, cyclin B, and cyclin-dependent kinase-1 (CDK1), which control the G2-to-M phase transition in the cell cycle. In addition, apigenin upregulated p21(WAF1/CIP1) and increased the interaction of p21(WAF1/CIP1) with proliferating cell nuclear antigen (PCNA), which inhibits cell cycle progression. Furthermore, apigenin significantly inhibited histone deacetylase (HDAC) activity and induced histone H3 acetylation. The subsequent chromatin immunoprecipitation (ChIP) assay indicated that apigenin increased acetylation of histone H3 in the p21(WAF1/CIP1) promoter region, resulting in the increase of p21(WAF1/CIP1) transcription. In a tumor xenograft model, apigenin effectively delayed tumor growth. In these apigenin-treated tumors, we also observed reductions in the levels of cyclin A and cyclin B and increases in the levels of p21(WAF1/CIP1) and acetylated histone H3. These findings demonstrate for the first time that apigenin can be used in breast cancer prevention and treatment through epigenetic regulation. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 434-444, 2017.

  16. Large ice particles associated with small ice water content observed by AIM CIPS imagery of polar mesospheric clouds: Evidence for microphysical coupling with small-scale dynamics

    Science.gov (United States)

    Rusch, D.; Thomas, G.; Merkel, A.; Olivero, J.; Chandran, A.; Lumpe, J.; Carstans, J.; Randall, C.; Bailey, S.; Russell, J.

    2017-09-01

    Observations by the Cloud Imaging and Particle Size (CIPS) instrument on the Aeronomy of Ice in the Mesosphere (AIM) satellite have demonstrated the existence of Polar Mesospheric Cloud (PMC) regions populated by particles whose mean sizes range between 60 and 100 nm (radii of equivalent volume spheres). It is known from numerous satellite experiments that typical mean PMC particle sizes are of the order of 40-50 nm. Determination of particle size by CIPS is accomplished by measuring the scattering of solar radiation at various scattering angles at a spatial resolution of 25 km2. In this size range we find a robust anti-correlation between mean particle size and albedo. These very-large particle-low-ice (VLP-LI) clouds occur over spatially coherent areas. The surprising result is that VLP-LI are frequently present either in the troughs of gravity wave-like features or at the edges of PMC voids. We postulate that an association with gravity waves exists in the low-temperature summertime mesopause region, and illustrate the mechanism by a gravity wave simulation through use of the 2D Community Aerosol and Radiation Model for Atmospheres (CARMA). The model results are consistent with a VLP-LI population in the cold troughs of monochromatic gravity waves. In addition, we find such events in Whole Earth Community Climate Model/CARMA simulations, suggesting the possible importance of sporadic downward winds in heating the upper cloud regions. This newly-discovered association enhances our understanding of the interaction of ice microphysics with dynamical processes in the upper mesosphere.

  17. DACH1 regulates cell cycle progression of myeloid cells through the control of cyclin D, Cdk 4/6 and p21{sup Cip1}

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Jae-Woong; Kim, Hyeng-Soo; Kim, Seonggon; Hwang, Junmo; Kim, Young Hun; Lim, Ga Young [School of Life Science and Biotechnology, Kyungpook National University, Daegu 702-701 (Korea, Republic of); Sohn, Wern-Joo [Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu 700-412 (Korea, Republic of); Yoon, Suk-Ran [Cell Therapy Research Center, Korea Research Institute of Bioscience and Biotechnology, Yuseong-gu, Daejeon 305-806 (Korea, Republic of); Kim, Jae-Young [Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu 700-412 (Korea, Republic of); Park, Tae Sung [Department of Laboratory Medicine, Kyung Hee University School of Medicine, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-702 (Korea, Republic of); Park, Kwon Moo [Department of Anatomy, Kyungpook National University School of Medicine, Daegu 700-422 (Korea, Republic of); Ryoo, Zae Young [School of Life Science and Biotechnology, Kyungpook National University, Daegu 702-701 (Korea, Republic of); Lee, Sanggyu, E-mail: slee@knu.ac.kr [School of Life Science and Biotechnology, Kyungpook National University, Daegu 702-701 (Korea, Republic of)

    2012-03-30

    Highlights: Black-Right-Pointing-Pointer DACH1 increases cyclin D, F and Cdk 1, 4, 6 in mouse myeloid progenitor cells. Black-Right-Pointing-Pointer The knockdown of DACH1 blocked the cell cycle progression of HL-60 cells. Black-Right-Pointing-Pointer The novel effect of DACH1 related with cell cycle regulation and leukemogenesis. -- Abstract: The cell-fate determination factor Dachshund, a component of the Retinal Determination Gene Network (RDGN), has a role in breast tumor proliferation through the repression of cyclin D1 and several key regulators of embryonic stem cell function, such as Nanog and Sox2. However, little is known about the role of DACH1 in a myeloid lineage as a cell cycle regulator. Here, we identified the differential expression levels of extensive cell cycle regulators controlled by DACH1 in myeloid progenitor cells. The forced expression of DACH1 induced p27{sup Kip1} and repressed p21{sup Cip1}, which is a pivotal characteristic of the myeloid progenitor. Furthermore, DACH1 significantly increased the expression of cyclin D1, D3, F, and Cdk 1, 4, and 6 in myeloid progenitor cells. The knockdown of DACH1 blocked the cell cycle progression of HL-60 promyeloblastic cells through the decrease of cyclin D1, D3, F, and Cdk 1, 4, and 6 and increase in p21{sup Cip1}, which in turn decreased the phosphorylation of the Rb protein. The expression of Sox2, Oct4, and Klf4 was significantly up-regulated by the forced expression of DACH1 in mouse myeloid progenitor cells.

  18. Urinary bladder lesions after the chernobyl accident. Immunohistochemical assessment of p53, proliferating cell nuclear antigen, cyclin D1 and p21[sup WAF1/Cip1

    Energy Technology Data Exchange (ETDEWEB)

    Romanenko, A.; Zaparin, W.; Vinnichenko, W.; Vozianov, A. (Academy of Medical Sciences of Ukraine, Kiev (Ukraine)); Lee, C.C.R.; Yamamoto, Shinji; Hori, Taka-aki; Wanibuchi, Hideki; Fukushima, Shoji

    1999-02-01

    During the 11-year period subsequent to the Chernobyl accident, the incidence of urinary bladder cancer in Ukraine has increased from 26.2 to 36.1 per 100,000 population. Cesium-137 ([sup 137]Cs) accounts for 80-90% of the incorporated radioactivity in this population, which has been exposed to long-term, low-dose ionizing radiation, and 80% of the more labile pool of cesium is excreted via the urine. The present study was performed to evaluate the histopathological features and the immunohistochemical status of p53, p21[sup WAF1/Cip1], cyclin D1 and PCNA (proliferating cell nuclear antigen) in urinary bladder mucosa of 55 males (49-92 years old) with benign prostatic hyperplasia who underwent surgery in Kiev, Ukraine, in 1995 and 1996. Group I (28 patients) inhabiting radiocontaminated areas of the country, group II (17 patients) from Kiev city with less radiocontamination and a control group III (10 patients) living in so-called ''clean'' areas of Ukraine were compared. In groups I and II, an increase in multiple areas of moderate or severe dysplasia or carcinoma in situ was seen in 42 (93%) of 45 cases. In addition, two small transitional cell carcinomas were found in one patient in each of groups I and II. Nuclear accumulation of p53, PCNA, cyclin D1, and to a lesser extent p21[sup WAF1/Cip1], was significantly increased in both groups I and II as compared with the control group III, indicating possible transformation events or enhancement of repair activities, that may precede the defect in the regulatory pathway itself, at least in the G1 phase of the cell cycle. Our results suggest that early malignant transformation is taking place in the bladder urothelium of people in the radiocontaminated areas of Ukraine and that this could possibly lead sometime in the future to an increased incidence of urinary bladder cancer. (author)

  19. 2010 Mississippi Curriculum Framework: Postsecondary Banking and Finance Technology. (Program CIP: 52.0803 - Banking and Financial Support Services)

    Science.gov (United States)

    Gullett, Janet; Dinkins, Shivochie

    2010-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  20. 2008 Mississippi Curriculum Framework: Postsecondary Radiologic Technology. (Program CIP: 51.0911 - Radiologic Technology/Science - Radiographer)

    Science.gov (United States)

    Armstrong, David; Cochran, Timothy; Compton, Steve; Davis, Jennifer; Edgerton, Seena Shazowee; Kisner, Christie; Lewis, Judy; Sartin, Billie Faye; Shell, Deborah

    2008-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  1. 2011 Mississippi Curriculum Framework: Postsecondary Dental Assisting Technology. (Program CIP: 51.0601 - Dental Assisting/Assistant)

    Science.gov (United States)

    Addison, Emily; Bokros, Christy; Gavant, H. Richard; Johnson, Robin

    2011-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  2. 2008 Mississippi Curriculum Framework: Postsecondary Practical Nursing. (Program CIP: 51.3901 - Licensed Practical/Vocational Nurse Training)

    Science.gov (United States)

    McNutt, Jana; Clowers, Pat; Collum, Lara; Canton, Dixie; Comfort, Sherri; Kron, Maxine; Mahaffey, Elizabeth; Hancock, Jane; Waldrup, Sandra; Walker, Sherri; Pearson, Lisa; Miller, Kelly; Cooper, Patti; Bedwell, Susan

    2008-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  3. 2010 Mississippi Curriculum Framework: Postsecondary Meat Merchandising Technology. (Program CIP: 12.0506 - Meat Cutting/Meat Cutter)

    Science.gov (United States)

    Jones, Roger; Currie, Lamar; Clayton, Sheila

    2010-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  4. 2008 Mississippi Curriculum Framework: Postsecondary Food Production and Management Technology. (Program CIP: 12.0508 - Institutional Food Workers)

    Science.gov (United States)

    Carver, Matthew; Hoff, Jody; Little, Lisa; Samuel, Carolyn

    2008-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  5. 2008 Mississippi Curriculum Framework: Postsecondary Irrigation Management Technology. (Program CIP:01.0699 - Applied Horticulture/Horticultural Business Services, Other)

    Science.gov (United States)

    Oliver, Michael L.

    2008-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  6. 2009 Mississippi Curriculum Framework: Postsecondary Funeral Service Technology. (Program CIP-12.0301 - Funeral Service and Mortuary Science)

    Science.gov (United States)

    Anderson, Larry; Dickerson, Octavia; Harvey, Bill; Moore, Tony

    2009-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  7. 2005 Mississippi Curriculum Framework: Postsecondary Banking and Finance Technology. (Program CIP: 52.0803 - Banking and Financial Support Services)

    Science.gov (United States)

    Gullett, Janet; Thigpen, Herbert

    2005-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  8. 2007 Mississippi Curriculum Framework: Postsecondary Meat Merchandising Technology (Program CIP: 12.0506 - Meat Cutting/Meat Cutter)

    Science.gov (United States)

    Jones, Roger; Currie, Lamar; Clayton, Sheila

    2007-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  9. 2010 Mississippi Curriculum Framework: Postsecondary Emergency Medical Technician. (Program CIP: 51.0904 - Emergency Medical Technology/Technician)

    Science.gov (United States)

    Briscoe, Lisa; Bryant, Katrina; Galtelli, Mark; Glasson, Kristi; Hall, David; Hood, Brenda; Mahaffey, Libby; McBryde, John; Read, John; Shirley, Gary; Wright, Al

    2010-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  10. 2008 Mississippi Curriculum Framework: Postsecondary Massage Therapy. (Program CIP: 51.3501 - Massage Therapy/Therapeutic Massage)

    Science.gov (United States)

    Patterson, Teresa; Russell, Cydnee

    2008-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  11. 2009 Mississippi Curriculum Framework: Postsecondary Nuclear Medicine Technology. (Program CIP: 51.0905 - Nuclear Medical Technology/Technologist)

    Science.gov (United States)

    Boney, Linda; Lee, Joanne; Pyles, Alice; Whitfield, Stacy

    2009-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  12. Epstein-Barr virus nuclear antigen 3A promotes cellular proliferation by repression of the cyclin-dependent kinase inhibitor p21WAF1/CIP1.

    Science.gov (United States)

    Tursiella, Melissa L; Bowman, Emily R; Wanzeck, Keith C; Throm, Robert E; Liao, Jason; Zhu, Junjia; Sample, Clare E

    2014-10-01

    Latent infection by Epstein-Barr virus (EBV) is highly associated with the endemic form of Burkitt lymphoma (eBL), which typically limits expression of EBV proteins to EBNA-1 (Latency I). Interestingly, a subset of eBLs maintain a variant program of EBV latency - Wp-restricted latency (Wp-R) - that includes expression of the EBNA-3 proteins (3A, 3B and 3C), in addition to EBNA-1. In xenograft assays, Wp-R BL cell lines were notably more tumorigenic than their counterparts that maintain Latency I, suggesting that the additional latency-associated proteins expressed in Wp-R influence cell proliferation and/or survival. Here, we evaluated the contribution of EBNA-3A. Consistent with the enhanced tumorigenic potential of Wp-R BLs, knockdown of EBNA-3A expression resulted in abrupt cell-cycle arrest in G0/G1 that was concomitant with conversion of retinoblastoma protein (Rb) to its hypophosphorylated state, followed by a loss of Rb protein. Comparable results were seen in EBV-immortalized B lymphoblastoid cell lines (LCLs), consistent with the previous observation that EBNA-3A is essential for sustained growth of these cells. In agreement with the known ability of EBNA-3A and EBNA-3C to cooperatively repress p14(ARF) and p16(INK4a) expression, knockdown of EBNA-3A in LCLs resulted in rapid elevation of p14(ARF) and p16I(NK4a). By contrast, p16(INK4a) was not detectably expressed in Wp-R BL and the low-level expression of p14(ARF) was unchanged by EBNA-3A knockdown. Amongst other G1/S regulatory proteins, only p21(WAF1/CIP1), a potent inducer of G1 arrest, was upregulated following knockdown of EBNA-3A in Wp-R BL Sal cells and LCLs, coincident with hypophosphorylation and destabilization of Rb and growth arrest. Furthermore, knockdown of p21(WAF1/CIP1) expression in Wp-R BL correlated with an increase in cellular proliferation. This novel function of EBNA-3A is distinct from the functions previously described that are shared with EBNA-3C, and likely contributes to the

  13. p21/Cip1 and p27/Kip1 Are essential molecular targets of inositol hexaphosphate for its antitumor efficacy against prostate cancer.

    Science.gov (United States)

    Roy, Srirupa; Gu, Mallikarjuna; Ramasamy, Kumaraguruparan; Singh, Rana P; Agarwal, Chapla; Siriwardana, Sunitha; Sclafani, Robert A; Agarwal, Rajesh

    2009-02-01

    Inositol hexaphosphate (IP6) causes G(1) arrest and increases cyclin-dependent kinase inhibitors p21/Cip1 and p27/Kip1 protein levels in human prostate cancer (PCa) DU145 cells lacking functional p53. However, whether cyclin-dependent kinase inhibitor I induction by IP6 plays any role in its antitumor efficacy is unknown. Herein, we observed that either p21 or p27 knockdown by small interfering RNA has no considerable effect on IP6-induced G(1) arrest, growth inhibition, and death in DU145 cells; however, the simultaneous knockdown of both p21 and p27 reversed the effects of IP6. To further confirm these findings both in vitro and in vivo, we generated DU145 cell variants with knockdown levels of p21 (DU-p21), p27 (DU-p27), or both (DU-p21+p27) via retroviral transduction of respective short hairpin RNAs. Knocking down p21 or p27 individually did not alter IP6-caused cell growth inhibition and G(1) arrest; however, their simultaneous ablation completely reversed the effects of IP6. In tumor xenograft studies, IP6 (2% w/v, in drinking water) caused a comparable reduction in tumor volume (40-46%) and tumor cell proliferation (26-28%) in DU-EV (control), DU-p21, and DU-p27 tumors but lost most of its effect in DU-p21+p27 tumors. IP6-caused apoptosis also occurred in a Cip/Kip-dependent manner because DU-p21+p27 cells were completely resistant to IP6-induced apoptosis both in cell culture and xenograft. Together, these results provide evidence, for the first time, of the critical role of p21 and p27 in mediating the anticancer efficacy of IP6, and suggest their redundant role in the antiproliferative and proapoptotic effects of IP6 in p53-lacking human PCa cells, both in vitro and in vivo.

  14. Epstein-Barr virus nuclear antigen 3A promotes cellular proliferation by repression of the cyclin-dependent kinase inhibitor p21WAF1/CIP1.

    Directory of Open Access Journals (Sweden)

    Melissa L Tursiella

    2014-10-01

    Full Text Available Latent infection by Epstein-Barr virus (EBV is highly associated with the endemic form of Burkitt lymphoma (eBL, which typically limits expression of EBV proteins to EBNA-1 (Latency I. Interestingly, a subset of eBLs maintain a variant program of EBV latency - Wp-restricted latency (Wp-R - that includes expression of the EBNA-3 proteins (3A, 3B and 3C, in addition to EBNA-1. In xenograft assays, Wp-R BL cell lines were notably more tumorigenic than their counterparts that maintain Latency I, suggesting that the additional latency-associated proteins expressed in Wp-R influence cell proliferation and/or survival. Here, we evaluated the contribution of EBNA-3A. Consistent with the enhanced tumorigenic potential of Wp-R BLs, knockdown of EBNA-3A expression resulted in abrupt cell-cycle arrest in G0/G1 that was concomitant with conversion of retinoblastoma protein (Rb to its hypophosphorylated state, followed by a loss of Rb protein. Comparable results were seen in EBV-immortalized B lymphoblastoid cell lines (LCLs, consistent with the previous observation that EBNA-3A is essential for sustained growth of these cells. In agreement with the known ability of EBNA-3A and EBNA-3C to cooperatively repress p14(ARF and p16(INK4a expression, knockdown of EBNA-3A in LCLs resulted in rapid elevation of p14(ARF and p16I(NK4a. By contrast, p16(INK4a was not detectably expressed in Wp-R BL and the low-level expression of p14(ARF was unchanged by EBNA-3A knockdown. Amongst other G1/S regulatory proteins, only p21(WAF1/CIP1, a potent inducer of G1 arrest, was upregulated following knockdown of EBNA-3A in Wp-R BL Sal cells and LCLs, coincident with hypophosphorylation and destabilization of Rb and growth arrest. Furthermore, knockdown of p21(WAF1/CIP1 expression in Wp-R BL correlated with an increase in cellular proliferation. This novel function of EBNA-3A is distinct from the functions previously described that are shared with EBNA-3C, and likely contributes to

  15. 啤酒酵母抽提物生产中CIP系统的配置及应用%The design and application of the CIP system in brewer's yeast extraction production

    Institute of Scientific and Technical Information of China (English)

    邵伟; 乐超银; 韩晓凯; 宋丽燕; 史咏华

    2008-01-01

    原地清洗(CIP)系统是一种不用拆卸设备、零部件和管道,不用进行人工擦洗和冲刷,而是通过清洗液的理化作用直接清洗设备和管道本身的方法.文中论述了原地清洗(CIP)系统的构成、影响CIP清洗效果的因素及CIP系统的设计要点,并结合酵母抽提物生产工艺实际,说明了CIP的应用情况.

  16. Alterations in TP53, cyclin D2, c-Myc, p21WAF1/CIP1 and p27KIP1 expression associated with progression in B-CLL

    Directory of Open Access Journals (Sweden)

    Antosz Halina

    2010-04-01

    Full Text Available B-cell chronic lymphocytic leukaemia (B-CLL originates from B lymphocytes that may differ in the activationlevel, maturation state or cellular subgroups in peripheral blood. Tumour progression in CLL B cells seems to result in gradualaccumulation of the clone of resting B lymphocytes in the early phases (G0/G1 of the cell cycle. The G1 phase isimpaired in B-CLL. We investigated the gene expression of five key cell cycle regulators: TP 53, c-Myc, cyclin D2,p21WAF1/CIP1 and p27KIP1, which primarily regulate the G1 phase of the cell cycle, or S-phase entry and ultimately controlthe proliferation and cell growth as well as their role in B-CLL progression. The study was conducted in peripheral bloodCLL lymphocytes of 40 previously untreated patients. Statistical analysis of correlations of TP53, cyclin D2, c-Myc,p21WAF1/CIP1 and p27KIP1 expressions in B-CLL patients with different Rai stages demonstrated that the progression of diseasewas accompanied by increases in p53, cyclin D2 and c-Myc mRNA expression. The expression of p27KIP1 was nearlystatistically significant whereas that of p21 WAF1/CIP1 showed no such correlation. Moreover, high expression levels of TP53and c-Myc genes were found to be closely associated with more aggressive forms of the disease requiring earlier therapy.

  17. Parasites of domestic and wild canids in the region of Serra do Cipó National Park, Brazil Parasitos de canídeos domésticos e silvestres na região do Parque Nacional da Serra do Cipó - Brasil

    Directory of Open Access Journals (Sweden)

    Juliana Lúcia Costa Santos

    2012-09-01

    Full Text Available Over recent decades, diseases have been shown to be important causes of extinctions among wild species. Greater emphasis has been given to diseases transmitted by domestic animals, which have been increasing in numbers in natural areas, along with human populations. This study had the aim of investigating the presence of intestinal helminths in wild canids (maned wolf, Chrysocyon brachyurus, and crab-eating fox, Cerdocyon thous in the Serra do Cipó National Park (43-44º W and 19-20º S and endo and ectoparasites of domestic dogs in the Morro da Pedreira Environmental Protection Area (an area surrounding the National Park. The Serra do Cipó is located in the state of Minas Gerais, Brazil. Among the enteroparasites found in domestic and wild canids, the following taxons were identified: Ancylostomidae, Trichuridae, Toxocara sp., Spirocerca sp., Physaloptera sp., Strongyloides sp., Cestoda, Dipylidium caninum, Diphyllobothriidae, Hymenolepidae, Anoplocephalidae, Trematoda, Acanthocephala and Isospora sp. Domestic dogs were positive for leishmaniasis and Babesia canis in serological tests. Among the ectoparasites, Rhipicephalus sanguineus, Amblyomma cajennense and Ctenocephalides felis felis were observed in domestic dogs. Variations in the chaetotaxy of the meta-episternum and posterior tibia were observed in some specimens of C. felis felis.Nas últimas décadas, as doenças têm sido apontadas como importantes causas de extinção de espécies silvestres. Maior ênfase tem sido dada às doenças transmitidas por animais domésticos que crescem em número, bem como as populações humanas, em áreas naturais. O presente estudo objetivou verificar a presença de helmintos intestinais de canídeos silvestres (lobo-guará - Chrysocyon brachyurus e cachorro-do-mato - Cerdocyon thous do Parque Nacional da Serra do Cipó (43-44º W e 19-20º S e endo e ectoparasitos de cães domésticos da Área de Proteção Ambiental Morro da Pedreira (entorno do

  18. Evaluación agronómica de diez clones promisorios CIP y dos materiales nativos de Ipomoea batatas L.

    Directory of Open Access Journals (Sweden)

    Tique José

    2009-08-01

    Full Text Available

    Con el propósito de rescatar la riqueza genética de la batata (Ipomoea batatas, durante dos ciclos de cultivo se realizaron evaluaciones agronómicas de dos materiales nativos, en comparación con diez clones provenientes del Centro Internacional de la Papa, clasificados allí como promisorios. El trabajo se llevó a cabo en el municipio de Coyaima, en el departamento del Tolima (Colombia, con la participación de los integrantes del Cabildo Indígena Autónomo Independiente Buenavista, y la colaboración del Consorcio Latinoamericano y del Caribe de Apoyo a la Investigación y el Desarrollo de la Yuca. Los materiales nativos se colectaron en parcelas de agricultores y se clasificaron como ‘Bonanza’ y ‘Sangretoro’, nombres tradicionales dados por los indígenas de la zona. El experimento fue desarrollado a través de un diseño de bloques completos al azar, con siete tratamientos y cuatro repeticiones. Los tratamientos correspondieron a los cinco clones CIP y las dos variedades regionales. Los resultados de la investigación permitieron cuantificar el alto potencial productivo de la batata como especie cultivada, encontrándose materiales muy promisorios que pueden ser incorporados a modelos agropecuarios de producción.

  19. TATA-binding protein (TBP)-like protein is required for p53-dependent transcriptional activation of upstream promoter of p21Waf1/Cip1 gene.

    Science.gov (United States)

    Suzuki, Hidefumi; Ito, Ryo; Ikeda, Kaori; Tamura, Taka-Aki

    2012-06-01

    TATA-binding protein-like protein (TLP) is involved in development, checkpoint, and apoptosis through potentiation of gene expression. TLP-overexpressing human cells, especially p53-containing cells, exhibited a decreased growth rate and increased proportion of G(1) phase cells. TLP stimulated expression of several growth-related genes including p21 (p21(Waf1/Cip1)). TLP-mediated activation of the p21 upstream promoter in cells was shown by a promoter-luciferase reporter assay. The p53-binding sequence located in the p21 upstream promoter and p53 itself are required for TLP-mediated transcriptional activation. TLP and p53 bound to each other and synergistically enhanced activity of the upstream promoter. TLP specifically activated transcription from the endogenous upstream promoter, and p53 was required for this activation. Etoposide treatment also resulted in activation of the upstream promoter as well as nuclear accumulation of TLP and p53. Moreover, the upstream promoter was associated with endogenous p53 and TLP, and the p53 recruitment was enhanced by TLP. The results of the present study suggest that TLP mediates p53-governed transcriptional activation of the p21 upstream promoter.

  20. Translational Upregulation of an Individual p21Cip1 Transcript Variant by GCN2 Regulates Cell Proliferation and Survival under Nutrient Stress.

    Directory of Open Access Journals (Sweden)

    Stacey L Lehman

    2015-06-01

    Full Text Available Multiple transcripts encode for the cell cycle inhibitor p21(Cip1. These transcripts produce identical proteins but differ in their 5' untranslated regions (UTRs. Although several stresses that induce p21 have been characterized, the mechanisms regulating the individual transcript variants and their functional significance are unknown. Here we demonstrate through (35S labeling, luciferase reporter assays, and polysome transcript profiling that activation of the Integrated Stress Response (ISR kinase GCN2 selectively upregulates the translation of a p21 transcript variant containing 5' upstream open reading frames (uORFs through phosphorylation of the eukaryotic translation initiation factor eIF2α. Mutational analysis reveals that the uORFs suppress translation under basal conditions, but promote translation under stress. Functionally, ablation of p21 ameliorates G1/S arrest and reduces cell survival in response to GCN2 activation. These findings uncover a novel mechanism of p21 post-transcriptional regulation, offer functional significance for the existence of multiple p21 transcripts, and support a key role for GCN2 in regulating the cell cycle under stress.

  1. Lenalidomide inhibits the proliferation of CLL cells via a cereblon/p21(WAF1/Cip1)-dependent mechanism independent of functional p53.

    Science.gov (United States)

    Fecteau, Jessie-F; Corral, Laura G; Ghia, Emanuela M; Gaidarova, Svetlana; Futalan, Diahnn; Bharati, Ila Sri; Cathers, Brian; Schwaederlé, Maria; Cui, Bing; Lopez-Girona, Antonia; Messmer, Davorka; Kipps, Thomas J

    2014-09-04

    Lenalidomide has demonstrated clinical activity in patients with chronic lymphocytic leukemia (CLL), even though it is not cytotoxic for primary CLL cells in vitro. We examined the direct effect of lenalidomide on CLL-cell proliferation induced by CD154-expressing accessory cells in media containing interleukin-4 and -10. Treatment with lenalidomide significantly inhibited CLL-cell proliferation, an effect that was associated with the p53-independent upregulation of the cyclin-dependent kinase inhibitor, p21(WAF1/Cip1) (p21). Silencing p21 with small interfering RNA impaired the capacity of lenalidomide to inhibit CLL-cell proliferation. Silencing cereblon, a known molecular target of lenalidomide, impaired the capacity of lenalidomide to induce expression of p21, inhibit CD154-induced CLL-cell proliferation, or enhance the degradation of Ikaros family zinc finger proteins 1 and 3. We isolated CLL cells from the blood of patients before and after short-term treatment with low-dose lenalidomide (5 mg per day) and found the leukemia cells were also induced to express p21 in vivo. These results indicate that lenalidomide can directly inhibit proliferation of CLL cells in a cereblon/p21-dependent but p53-independent manner, at concentrations achievable in vivo, potentially contributing to the capacity of this drug to inhibit disease-progression in patients with CLL.

  2. Lenalidomide inhibits the proliferation of CLL cells via a cereblon/p21WAF1/Cip1-dependent mechanism independent of functional p53

    Science.gov (United States)

    Fecteau, Jessie-F.; Corral, Laura G.; Ghia, Emanuela M.; Gaidarova, Svetlana; Futalan, Diahnn; Bharati, Ila Sri; Cathers, Brian; Schwaederlé, Maria; Cui, Bing; Lopez-Girona, Antonia; Messmer, Davorka

    2014-01-01

    Lenalidomide has demonstrated clinical activity in patients with chronic lymphocytic leukemia (CLL), even though it is not cytotoxic for primary CLL cells in vitro. We examined the direct effect of lenalidomide on CLL-cell proliferation induced by CD154-expressing accessory cells in media containing interleukin-4 and -10. Treatment with lenalidomide significantly inhibited CLL-cell proliferation, an effect that was associated with the p53-independent upregulation of the cyclin-dependent kinase inhibitor, p21WAF1/Cip1 (p21). Silencing p21 with small interfering RNA impaired the capacity of lenalidomide to inhibit CLL-cell proliferation. Silencing cereblon, a known molecular target of lenalidomide, impaired the capacity of lenalidomide to induce expression of p21, inhibit CD154-induced CLL-cell proliferation, or enhance the degradation of Ikaros family zinc finger proteins 1 and 3. We isolated CLL cells from the blood of patients before and after short-term treatment with low-dose lenalidomide (5 mg per day) and found the leukemia cells were also induced to express p21 in vivo. These results indicate that lenalidomide can directly inhibit proliferation of CLL cells in a cereblon/p21-dependent but p53-independent manner, at concentrations achievable in vivo, potentially contributing to the capacity of this drug to inhibit disease-progression in patients with CLL. PMID:24990888

  3. Translational Upregulation of an Individual p21Cip1 Transcript Variant by GCN2 Regulates Cell Proliferation and Survival under Nutrient Stress.

    Directory of Open Access Journals (Sweden)

    Stacey L Lehman

    2015-06-01

    Full Text Available Multiple transcripts encode for the cell cycle inhibitor p21(Cip1. These transcripts produce identical proteins but differ in their 5' untranslated regions (UTRs. Although several stresses that induce p21 have been characterized, the mechanisms regulating the individual transcript variants and their functional significance are unknown. Here we demonstrate through (35S labeling, luciferase reporter assays, and polysome transcript profiling that activation of the Integrated Stress Response (ISR kinase GCN2 selectively upregulates the translation of a p21 transcript variant containing 5' upstream open reading frames (uORFs through phosphorylation of the eukaryotic translation initiation factor eIF2α. Mutational analysis reveals that the uORFs suppress translation under basal conditions, but promote translation under stress. Functionally, ablation of p21 ameliorates G1/S arrest and reduces cell survival in response to GCN2 activation. These findings uncover a novel mechanism of p21 post-transcriptional regulation, offer functional significance for the existence of multiple p21 transcripts, and support a key role for GCN2 in regulating the cell cycle under stress.

  4. SOX9 accelerates ESC differentiation to three germ layer lineages by repressing SOX2 expression through P21 (WAF1/CIP1).

    Science.gov (United States)

    Yamamizu, Kohei; Schlessinger, David; Ko, Minoru S H

    2014-11-01

    Upon removal of culture conditions that maintain an undifferentiated state, mouse embryonic stem cells (ESCs) differentiate into various cell types. Differentiation can be facilitated by forced expression of certain transcription factors (TFs), each of which can generally specify a particular developmental lineage. We previously established 137 mouse ESC lines, each of which carried a doxycycline-controllable TF. Among them, Sox9 has unique capacity: its forced expression accelerates differentiation of mouse ESCs into cells of all three germ layers. With the additional use of specific culture conditions, overexpression of Sox9 facilitated the generation of endothelial cells, hepatocytes and neurons from ESCs. Furthermore, Sox9 action increases formation of p21 (WAF1/CIP1), which then binds to the SRR2 enhancer of pluripotency marker Sox2 and inhibits its expression. Knockdown of p21 abolishes inhibition of Sox2 and Sox9-accelerated differentiation, and reduction of Sox2 2 days after the beginning of ESC differentiation can comparably accelerate mouse ESC formation of cells of three germ layers. These data implicate the involvement of the p21-Sox2 pathway in the mechanism of accelerated ESC differentiation by Sox9 overexpression. The molecular cascade could be among the first steps to program ESC differentiation.

  5. Anti-cancer effects of p21WAF1/CIP1 transcriptional activation induced by dsRNAs in human hepatocellular carcinoma cell lines

    Institute of Scientific and Technical Information of China (English)

    Zhi-ming WU; Gang CHEN; Chun DAI; Ying HUANG; Cui-fang ZHENG; Qiong-zhu DONG; Guan WANG; Xiao-wen LI; Xiao-fei ZHANG; Bin LI

    2011-01-01

    Aim: To investigate the anti-cancer effects of p21WAF1/CIP1 transcriptional activation induced by dsRNAs in hepatocellular carcinoma (HCC) cell lines.Methods: HCC cell lines BEL7402, SMMC-7721, MHCC97L, MHCC97H, and MHCCLM3 were used. HCC ceils were treated with dsP21322 (50 nmol/L), dsControl (50 nmol/L), siP21 (50 nmol/L), or mock transfection. The expression of p21 was detected using quantitative PCR and Western blot. The effects of RNA activation on HCC cells were determined using cell viability assays, apoptosis analyses and clonogenic survival assays. Western blot was also conducted to detect the expression of Bcl-xL, survivin, cleaved caspase-3,cleaved caspase-9 and cleaved PARP.Results: At 72 to 120 h following the transfection, dsP21-322 markedly inhibited the viability of HCC cells and clone formation. At the same times, dsP21-322 caused a significant increase in HCC cell apoptosis, as demonstrated with cytometric analysis. The phenomena were correlated with decreased expression levels of the anti-apoptotic proteins Bcl-xL, surviving, and increased expression of cleaved caspase-3, cleaved caspase-9 and cleaved PARP.Conclusion: RNA-induced activation of p21 gene expression may have significant therapeutic potential for the treatment of hepatocellular carcinoma and other cancers.

  6. 基于CIPS的DEM匹配的实验研究%The Experimental Study of DEM Matching Based on the CIPS

    Institute of Scientific and Technical Information of China (English)

    陈凤

    2012-01-01

    With the rapid development of city construction and the more and more pressing need of basic geographic information caused by urbanization, Digital Elevation Model is becoming one of the indispensable kinds of basic geographic information, drawing attention to itself in the field of photogrammetric survey with the introduction of ADS 40 push - broom digital sensor. This paper carries out experimental research and precision analysis on DEM matching based on CIPS taking ADS 40 image data of a certain area as a case study.%随着城市建设的快速发展,城市化对基础地理信息的需要日趋迫切,数字高程模型(Digital Elevation Model,DEM)成为不可缺少的基础地理信息之一,并随着ADS40推扫式数字传感器的推广在摄影测量界备受关注。文章以某地区的ADS40影像数据为例,基于CIPS进行了DEM匹配的实验研究与精度分析。

  7. Structural Behavior of Continuous Prestressed Steel Fiber Reinforced High Strength Concrete Beam

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    The flexural behaviors of continuous fully and partially prestressed steel fiber reinforced high strength concrete beams are studied by experiment and nonlinear finite element analysis. Three levels of partial prestress ratio (PPR) are considered, and three pairs of two-span continuous beams with box sections varying in size are designed. The major parameters involved in the study include the PPR and the fiber location. It is concluded that the prestressed high strength concrete beam exhibits satisfactory ductility; the influences of steel fiber on the crack behaviors for partially prestressed beams are not as obvious as those for fully prestressed ones; steel fibers can improve the structural stiffness after cracking for fully prestressed high strength concrete beams; the moment redistribution from mid-span to intermediate support in the first stage should be mainly considered in practical design.

  8. {sup 123}I-labeled HIV-1 tat peptide radioimmunoconjugates are imported into the nucleus of human breast cancer cells and functionally interact in vitro and in vivo with the cyclin-dependent kinase inhibitor, p21{sup WAF-1/Cip-1}

    Energy Technology Data Exchange (ETDEWEB)

    Hu, Meiduo [University Health Network, Division of Nuclear Medicine, Toronto, ON (Canada); University of Toronto, Department of Pharmaceutical Sciences, Toronto, ON (Canada); Chen, Paul; Wang, Judy; Scollard, Deborah A. [University Health Network, Division of Nuclear Medicine, Toronto, ON (Canada); Vallis, Katherine A. [University Health Network, Department of Radiation Oncology, Toronto, ON (Canada); University of Toronto, Department of Medical Biophysics, Toronto, ON (Canada); Reilly, Raymond M. [University Health Network, Division of Nuclear Medicine, Toronto, ON (Canada); University of Toronto, Department of Pharmaceutical Sciences, Toronto, ON (Canada); University of Toronto, Department of Medical Imaging, Toronto, ON (Canada); University of Toronto, Leslie Dan Faculty of Pharmacy, Toronto, ON (Canada)

    2007-03-15

    To evaluate the internalization and nuclear translocation of {sup 123}I-tat-peptide radioimmunoconjugates in MDA-MB-468 breast cancer cells and their ability to interact with the cyclin-dependent kinase inhibitor, p21{sup WAF-1/Cip-1}. Peptides [GRKKRRQRRRPPQGYGC] harboring the nuclear-localizing sequence from HIV tat domain were conjugated to anti-p21{sup WAF-1/Cip-1} antibodies. Immunoreactivity was assessed by Western blot using lysate from MDA-MB-468 cells exposed to EGF to induce p21{sup WAF-1/Cip-1}. Internalization and nuclear translocation were measured. The ability of tat-anti-p21{sup WAF-1/Cip-1} to block G{sub 1}-S phase arrest in MDA-MB-468 cells caused by EGF-induced p21{sup WAF-1/Cip-1} was evaluated. Tumor and normal tissue uptake were determined at 48 h p.i. in athymic mice implanted s.c. with MDA-MB-468 xenografts injected intratumorally with EGF. There was 13.4{+-}0.2% of radioactivity internalized by MDA-MB-468 cells incubated with {sup 123}I-tat-anti-p21{sup WAF-1/Cip-1} and 34.6{+-}3.1% imported into the nucleus. Tat-anti-p21{sup WAF-1/Cip-1}(8 {mu}M) decreased the proportion of EGF-treated cells in G{sub 1} phase from 81.9{+-}0.7% to 46.1{+-}0.7% (p<0.001), almost restoring the G{sub 1} phase fraction to that of unexposed cells (25.8{+-}0.2%). Non-specific tat-mouse IgG did not block EGF-induced G{sub 1}-S phase arrest. Tumor uptake of radioactivity was higher in mice injected with EGF to induce p21{sup WAF-1/Cip-1} than in mice not receiving EGF (3.1{+-}0.4% versus 1.8{+-}0.2% ID/g; p=0.04). Western blot analysis of tumors revealed a threefold increase in the p21{sup WAF-1/Cip-1}/{beta}-actin ratio. We conclude that intracellular and nuclear epitopes in cancer cells can be functionally targeted with tat-radioimmunoconjugates to exploit many more epitopes for imaging and radiotherapeutic applications than have previously been accessible. (orig.)

  9. P21Cip/WAF1及P27Kip1与增殖细胞核抗原在人骨髓间充质干细胞向肝细胞分化过程中的表达%Expression of P21Cip/WAF1,P27Kip1 and proliferating cell nuclear antigen during differentiation of human bone marrow mesenchymal stem cells into hepatocytes

    Institute of Scientific and Technical Information of China (English)

    汤志华; 闵军; 陈积圣; 叶林; 余杰雄

    2009-01-01

    背景:P21CIp/WAF1、P27Kip1是两种重要的细胞周期的负调控因子,增殖细胞核抗原是反映干细胞增殖的指标.目的:观察体外诱导人骨髓间充质干细胞向肝细胞分化过程中P21Cip/WAF1,P27KIp1及增殖细胞核抗原的表达变化.设计、时间及地点:细胞学基因水平观察,于2006-12/2007-06在中山大学附属第二医院林百欣医学研究中心实验室完成.材料:骨髓来源于中山大学附属第二医院行骨髓穿刺的健康供者.方法:全骨髓贴壁筛选法体外分离培养人骨髓间充质干细胞,传至第5代后,加入含20 μg/L肝细胞生长因子、10 μg/L成纤维细胞生长因子4的opti-MEM I低血清培养基向肝细胞诱导分化.主要观察指标:诱导后RT-PCR检测细胞内P21Cip/WAF1,P27 KIp1及增殖细胞核抗原基因mRNA的表达.结果:骨髓间充质干细胞向肝细胞定向诱导后0,1,3,5,7 d,细胞内P21CIp/WAF1 mRNA表达的相对量逐渐升高(P<0.01),P27Kip1 mRNA表达的相对量无明显差异(P>0.05),增殖细胞核抗原mRNA表达的相对量除诱导后0,1 d无明显差异(P>0.05)外,其余各时间点均逐渐降低(P<0.01).结论:P21 CIp/WAF1高表达抑制了骨髓间充质干细胞增殖,同时在其向肝细胞分化过程中起一定调控作用,P27KIp1可能并不起作用,增殖细胞核抗原的表达则与P21Cip/WAF1相反,呈逐步减少趋势.

  10. Discretization of Continuous Frame

    Indian Academy of Sciences (India)

    A Fattahi; H Javanshiri

    2012-05-01

    In this paper we consider the notion of continuous frame of subspaces and define a new concept of continuous frame, entitled continuous atomic resolution of identity, for arbitrary Hilbert space $\\mathcal{H}$ which has a countable reconstruction formula. Among the other results, we characterize the relationship between this new concept and other known continuous frames. Finally, we state and prove the assertions of the stability of perturbation in this concept.

  11. Level of reactive oxygen species induced by p21Waf1/CIP1 is critical for the determination of cell fate.

    Science.gov (United States)

    Inoue, Takafumi; Kato, Kiyoko; Kato, Hidenori; Asanoma, Kazuo; Kuboyama, Ayumi; Ueoka, Yousuke; Yamaguchi, Shin-ichiro; Ohgami, Tatsuhiro; Wake, Norio

    2009-07-01

    p21(WAF(1)/)(CIP(1)) is a well-known cell cycle regulatory protein which is overexpressed in several cancer cell lines, and known to determine cell fate. We generated three recombinant adenovirus vectors that expressed either the full-length p21 (Ad-p21F), a p21 mutant with a deletion of the C-terminal proliferative cell nuclear antigen (PCNA) binding domain (Ad-p21N), or a p21 mutant with a deletion of the N-terminal cyclin-dependent kinase binding domain (Ad-p21C). We transfected these vectors into five cancer cell lines. Premature senescence was induced in all of the lines only following transfection with Ad-p21N and Ad-p21F. In addition, apoptosis was also induced in LoVo and HCT116 cells that harbored wild-type p53 and the reactive oxygen species (ROS) level was higher than in senescent cells. Finally, the induction of apoptosis was inhibited by using siRNA to downregulate p53. This observation implies that there is a feedback signaling loop involving p21/ROS/p53 in apoptotic responses. It appears to be, at least in part, driven by high levels of p21 protein. Next, we investigated the cell death effect of endogenous p21 protein on cell fate using sodium butyrate (NaB). Treatment with 1 mM NaB or 2 to 5 mM NaB induced senescence or apoptosis, respectively. The level of intracellular ROS in 5 mM NaB treated cells was 2-fold higher, compared with that in 1 mM NaB treated cells. We also demonstrated that DNA damage response signals including ataxia telangiectasia mutated, gammaH2AX, and p38 MAPK were involved in NaB-induced cell death. The magnitude of intracellular ROS levels in response to p21 elicited either senescence or apoptosis in the cancer cell lines.

  12. Continuous Markovian Logics

    DEFF Research Database (Denmark)

    Mardare, Radu Iulian; Cardelli, Luca; Larsen, Kim Guldstrand

    2012-01-01

    Continuous Markovian Logic (CML) is a multimodal logic that expresses quantitative and qualitative properties of continuous-time labelled Markov processes with arbitrary (analytic) state-spaces, henceforth called continuous Markov processes (CMPs). The modalities of CML evaluate the rates of the ...

  13. Plants under continuous light

    NARCIS (Netherlands)

    Velez Ramirez, A.I.; Ieperen, van W.; Vreugdenhill, D.; Millenaar, F.F.

    2011-01-01

    Continuous light is an essential tool for understanding the plant circadian clock. Additionally, continuous light might increase greenhouse food production. However, using continuous light in research and practice has its challenges. For instance, most of the circadian clock-oriented experiments wer

  14. Projectbeschrijving Cadmium-informatiepunt (CIP)

    NARCIS (Netherlands)

    Meijer PJ

    1989-01-01

    To minimize the use of cadmium the Central Government has decided to perform the purchase of products and materials within the Central Government as much as possible within the Draft Cadmium Decree. The activities to achieve this are as far as could be seen at the start of the project in june

  15. O ensino da Classificação Internacional de Patentes (CIP nos cursos de biblioteconomia brasileiros La enseñanza de la Clasificación Internacional de Patentes nos cursos de bibliotecologia do Brasil

    Directory of Open Access Journals (Sweden)

    Joana Coeli Ribeiro Garcia

    2009-03-01

    Full Text Available Ao identificar em quais Cursos de Biblioteconomia a Classificação Internacional de Patentes (CIP é ensinada, pretende-se despertar nos profissionais da área a necessidade de conhecer ou de aprofundar tais conhecimentos. Os cursos, objeto do estudo, foram identificados por meio do site da Associação Brasileira de Educação em Ciência da Informação (ABECIN. A técnica utilizada é o survey, tendo o e-mail como instrumento de envio do questionário e recebimento das respostas que formam a amostra da pesquisa. O instrumento de pesquisa foi o questionário, formulado com perguntas abertas. Embora o documento de patente contenha informações relevantes para promoção do desenvolvimento tecnológico das nações, ainda não recebe um tratamento condizente das instituições brasileiras de ensino, ou seja, na maioria dos cursos em que se estudam formas de classificar o conhecimento, a CIP sequer é citada nas disciplinas.

  16. When rare is just a matter of sampling: unexpected dominance of clubtail dragonflies (Odonata, Gomphidae through different collecting methods at Parque Nacional da Serra do Cipó, Minas Gerais State, Brazil

    Directory of Open Access Journals (Sweden)

    Marcus Vinícius Oliveira de Almeida

    2013-12-01

    Full Text Available When rare is just a matter of sampling: Unexpected dominance of clubtail dragonflies (Odonata, Gomphidae through different collecting methods at Parque Nacional da Serra do Cipó, Minas Gerais State, Brazil. Capture of dragonfly adults during two short expeditions to Parque Nacional da Serra do Cipó, Minas Gerais State, using three distinct collecting methodsaerial nets, Malaise and light sheet trapsis reported. The results are outstanding due the high number of species of Gomphidae (7 out of 26 Odonata species, including a new species of Cyanogomphus Selys, 1873, obtained by two non-traditional collecting methods. Because active collecting with aerial nets is the standard approach for dragonfly inventories, we discuss some aspects of the use of traps, comparing our results with those in the literature, suggesting they should be used as complementary methods in faunistic studies. Furthermore, Zonophora campanulata annulata Belle, 1983 is recorded for the first time from Minas Gerais State and taxonomic notes about Phyllogomphoides regularis (Selys, 1873 and Progomphus complicatus Selys, 1854 are also given.

  17. Continuity in Discrete Sets

    CERN Document Server

    Burgin, Mark

    2010-01-01

    Continuous models used in physics and other areas of mathematics applications become discrete when they are computerized, e.g., utilized for computations. Besides, computers are controlling processes in discrete spaces, such as films and television programs. At the same time, continuous models that are in the background of discrete representations use mathematical technology developed for continuous media. The most important example of such a technology is calculus, which is so useful in physics and other sciences. The main goal of this paper is to synthesize continuous features and powerful technology of the classical calculus with the discrete approach of numerical mathematics and computational physics. To do this, we further develop the theory of fuzzy continuous functions and apply this theory to functions defined on discrete sets. The main interest is the classical Intermediate Value theorem. Although the result of this theorem is completely based on continuity, utilization of a relaxed version of contin...

  18. On barely continuous functions

    Directory of Open Access Journals (Sweden)

    Richard Stephens

    1988-01-01

    Full Text Available The term barely continuous is a topological generalization of Baire-1 according to F. Gerlits of the Mathematical Institute of the Hungarian Academy of Sciences, and thus worthy of further study. This paper compares barely continuous functions and continuous functions on an elementary level. Knowing how the continuity of the identity function between topologies on a given set yields the lattice structure for those topologies, the barely continuity of the identity function between topologies on a given set is investigated and used to add to the structure of that lattice. Included are certain sublattices generated by the barely continuity of the identity function between those topologies. Much attention is given to topologies on finite sets.

  19. Cutting Out Continuations

    DEFF Research Database (Denmark)

    Bahr, Patrick; Hutton, Graham

    2016-01-01

    In the field of program transformation, one often transforms programs into continuation-passing style to make their flow of control explicit, and then immediately removes the resulting continuations using defunctionalisation to make the programs first-order. In this article, we show how these two...... transformations can be fused together into a single transformation step that cuts out the need to first introduce and then eliminate continuations. Our approach is calculational, uses standard equational reasoning techniques, and is widely applicable....

  20. Immunoexpression of tumor suppressor genes p53, p21WAF1/CIP1 and p27KIP1 in humam astrocystic tumors Imuno-expressão dos genes supressores tumorais p53, p21WAF1/CIP1 e p27KIP1 em tumores astrocíticos humanos

    Directory of Open Access Journals (Sweden)

    Mário Henrique Girão Faria

    2007-12-01

    Full Text Available The aim of the present study was to evaluate the tumor suppressor genes p53, p21WAF1/CIP1 and p27KIP1 expression in astrocytic tumors, correlating the findings with the histopathological grade (WHO. An immunohistochemical study of the p53, p21 and p27 proteins using the streptavidin-biotin-peroxidase method was performed in fifty-five astrocytomas (13 grade I, 14 grade II, 7 grade III and 21 grade IV and five samples of non-tumor brain tissue (negative control. p53 positive indices (PI and labeling indices (LI showed tendency to increase according to malignant progression. The nuclear expression of p27 presented similar inclination, except for the PI reduction verified in grade IV tumors. Otherwise, the cytoplasmic p27 staining was more evident between high-grade tumors (III and IV. p53 and nuclear p27 expression was correlated with the histological classification (pO presente estudo objetivou avaliar a expressão dos supressores tumorais p53, p21WAF1/CIP1 e p27KIP1 em tumores astrocíticos humanos, correlacionando os achados com a graduação histopatológica (OMS. Procedeu-se o estudo imuno-histoquímico para as proteínas p53, p21 e p27 utilizando o método da estreptavidina-biotina-peroxidase em 55 astrocitomas (13 do grau I, 14 do grau II, 7 do grau III e 21 do grau IV e 5 amostras de tecido cerebral não-tumoral (controle negativo. Os índices de positividade (PI e de marcação (LI para p53 demonstraram tendência de aumento conforme a progressão maligna. A expressão nuclear do p27 apresentou semelhante inclinação, exceto pela redução do PI verificada nos tumores do grau IV. Já a marcação citoplasmática do p27 foi mais evidente entre tumores de alto grau (III e IV. As expressões de p53 e p27 nuclear demonstraram correlação com a classificação histológica (p<0,01; teste H. Por outro lado, os índices para p21 manifestaram propensão à redução conforme a evolução maligna dos tumores astrocíticos, salvo significante

  1. 叶酸通过调节p53/p21(waf1/cip1)信号途径促进小鼠神经干细胞增殖和分化%Folic acid promote proliferation and differentiation of neural stem cells via regulation p53/p21 ( waf1/cip1 ) pathway in mice

    Institute of Scientific and Technical Information of China (English)

    王虹; 王芳; 范利军; 包金风

    2012-01-01

    目的:研究叶酸对体外培养小鼠神经干细胞(neuralstemcells,NSCs)增殖和分化的影响及作用机制.方法:采用无血清悬浮培养方法分离培养新生小鼠脑NSCs,通过MTT法检测叶酸对NSCs增殖的影响;撤除生长因子后,用含10%胎牛血清的培养基诱导分化培养6d后,采用Tuj1(神经元标记物)和GFAP(胶质细胞标记物)免疫荧光双标记法检测叶酸对NSCs分化的影响;并应用流式细胞术、RT-PCR法检测给予叶酸对NSCs细胞周期、p53和p21(waf1/cip1)mRNA水平的影响.结果:与对照组相比,MTT法测定结果显示,叶酸组NSCs增殖能力明显增强;分化后免疫荧光双标法测定显示,叶酸组Tuj1阳性细胞的比率明显增加,且差异具有显著性(P<0.01);流式细胞仪测定结果显示,叶酸组NSCs在G0/G1期细胞数量明显减少(P<0.01),而G2/M期细胞数量明显增多(P<0.01);RT-PCR结果显示,叶酸组NSCs中p53和p21mRNA表达量明显降低.结论:叶酸能促进NSCs增殖及向神经元分化;叶酸对NSCs增殖和分化的影响与调节NSCs细胞周期及p53/p21(waf1/cip1)信号转导途径相关.%Objective: To explore the effects and mechanisms of folic acid on proliferation and differentiation of neural stem cells (NSCs) in vitro in mice. Methods: NSCs were isolated from newborn mice brain and suspension cultured in serum-free medium. The effect of folic acid on proliferation of NSCs was determinated by MTT method; the differentiation of NSCs was detected by immunofluorescence double labeled with Tujl (marker of neuron) and GFAP (marker of glia) after 6 day culture in 10% fetal calf serum medium condition without growth factors. The cell cycle of NSCs was detected by flow cytometry and the mRNA levels of p53 and p21 were determinated by RT-PCR. Results: The proliferation rate of NSCs andTujl positive cells were significantly higher in folic acid group than control group(/'< 0.01); the ratio of NSCs in G0/G1 phase was decreased (P

  2. Let Continuous Outcome Variables Remain Continuous

    Directory of Open Access Journals (Sweden)

    Enayatollah Bakhshi

    2012-01-01

    Full Text Available The complementary log-log is an alternative to logistic model. In many areas of research, the outcome data are continuous. We aim to provide a procedure that allows the researcher to estimate the coefficients of the complementary log-log model without dichotomizing and without loss of information. We show that the sample size required for a specific power of the proposed approach is substantially smaller than the dichotomizing method. We find that estimators derived from proposed method are consistently more efficient than dichotomizing method. To illustrate the use of proposed method, we employ the data arising from the NHSI.

  3. Residential Continuing Education.

    Science.gov (United States)

    Houle, Cyril O.

    The theme of this discursive essay is residential continuing education: its definition, its development along somewhat different lines in Europe and in America, and its practice in university centers in the United States. Continuing education includes any learning or teaching program that is based on the assumptions that the learners have studied…

  4. On continued fraction algorithms

    NARCIS (Netherlands)

    Smeets, Ionica

    2010-01-01

    Is there a good continued fraction approximation between every two bad ones? What is the entropy of the natural extension for alpha-Rosen fractions? How do you find multi-dimensional continued fractions with a guaranteed quality in polynomial time? These, and many more, questions are answered in thi

  5. On continued fraction algorithms

    NARCIS (Netherlands)

    Smeets, Ionica

    2010-01-01

    Is there a good continued fraction approximation between every two bad ones? What is the entropy of the natural extension for alpha-Rosen fractions? How do you find multi-dimensional continued fractions with a guaranteed quality in polynomial time? These, and many more, questions are answered in thi

  6. Study and Application of Gold Extraction Agent in a CIP Process%某提金剂炭浆提金试验研究及应用

    Institute of Scientific and Technical Information of China (English)

    刘金贵; 张淑英; 李政军

    2016-01-01

    In order to protect water resources,realize green development and gold extraction with environmental protection,it was tried to use ten pro-environment type regents for gold leaching,and selected better regents used for further industrial test.The results showed that one of the gold extraction regents had the advantages of environmental protection,fast procedure and easy operation.For example,leaching efficiency reached to70.47% for 747 g/t mineral aggregate,particularly for oxidized ore,leaching efficiency can even be up to75.16%.Thus,the pro-environment type regent was applied to CIP process for six months production.The results of practice in production demonstrated that this gold extraction regent can replace to NaCN,and leaching efficiency was about 72.92% for 650 g/t mineral aggregate,showing that the production cost reduced by 4.78 RMB/t and 0.57 million RMB/year.Obviously,this kind of gold extraction regent has the good economic benefit and remarkable environmental benefit,hence it can be applied in the gold industry in future.%为了保护水资源环境,实现绿色发展和环保提金,试验研究通过小试对10种新型环保提金剂进行浸出,从中选择效果较好的环保提金剂进行工业试验。工业试验结果表明:该提金剂具有环保、浸出速度快及使用方法简便的优点,吨矿耗量为747 g/t,金浸出率达70.47%,尤其对氧化矿浸出效果更好,浸出率可达75.16%。某提金剂应用于炭浆提金生产中,6个月生产实践结果表明:某提金剂完全可以替代 NaCN,吨矿耗量为650 g/t,金浸出率达到72.92%,单位成本可降低4.78元/t,生产成本可节省57万元/年,具有良好的经济效益且环境效益显著,可在黄金行业推广应用。

  7. A Importância da Caracterização dos Visitantes nas Ações de Ecoturismo e Educação Ambiental do Parque Nacional da Serra do Cipó-MG.

    Directory of Open Access Journals (Sweden)

    Fernanda Carla Wasner Vasconcelos

    2011-09-01

    Full Text Available

    O objetivo deste trabalho foi conhecer o perfil dos visitantes do Parque Nacional da Serra do Cipó de forma a contribuir para o planejamento do ecoturismo e de ações de educação ambiental neste destino. O Parque Nacional da Serra do Cipó se situa no distrito de mesmo nome, pertencente ao município de Santana do Riacho, distante cerca de 100 km de Belo Horizonte/MG. Foram analisados 53 questionários, aplicados nos dias 18 e 19 de setembro de 2010. Verificou-se que o perfil dos visitantes encaixa-se dentro do comumente proposto na literatura para ecoturistas. Predominam visitantes jovens, com renda elevada, alto nível de escolaridade, provenientes de Belo Horizonte e de sua Região Metropolitana. A maioria dos entrevistados reconhece os objetivos principais de um Parque, bem como a importância de um centro de visitantes e demonstra disposição a seguir regras de conservação da natureza e a participar de atividades de educação ambiental. Apesar de não possuírem um conceito formulado de ecoturismo, mais da metade dos visitantes se considera ecoturista. Recomenda-se assim, a utilização dos princípios do ecoturismo e, conseqüentemente, da educação ambiental, nas ações desenvolvidas pelo Parque Nacional da Serra do Cipó, em seu uso público. Dessa forma, acredita-se que o turismo possa ser verdadeiramente econômico, social, cultural e ecologicamente sustentável, além de fonte de conservação ambiental.

  8. Continuous Markovian Logics

    DEFF Research Database (Denmark)

    Mardare, Radu Iulian; Cardelli, Luca; Larsen, Kim Guldstrand

    2012-01-01

    Continuous Markovian Logic (CML) is a multimodal logic that expresses quantitative and qualitative properties of continuous-time labelled Markov processes with arbitrary (analytic) state-spaces, henceforth called continuous Markov processes (CMPs). The modalities of CML evaluate the rates...... characterizes stochastic bisimilarity and it supports the definition of a quantified extension of the satisfiability relation that measures the "compatibility" between a model and a property. In this context, the metaproperties allows us to prove two robustness theorems for the logic stating that one can...

  9. Nocturnal continuous glucose monitoring

    DEFF Research Database (Denmark)

    Bay, Christiane; Kristensen, Peter Lommer; Pedersen-Bjergaard, Ulrik;

    2013-01-01

    Abstract Background: A reliable method to detect biochemical nocturnal hypoglycemia is highly needed, especially in patients with recurrent severe hypoglycemia. We evaluated reliability of nocturnal continuous glucose monitoring (CGM) in patients with type 1 diabetes at high risk of severe...

  10. Continuous-time signals

    CERN Document Server

    Shmaliy, Yuriy

    2006-01-01

    Gives a modern description of continuous-time deterministic signals Signal formation techniquesTime vs. frequency and frequency vs. time analysisCorrelation and energy analysisNarrowband signals and sampling.

  11. Continuous ethanol fermentors

    Energy Technology Data Exchange (ETDEWEB)

    1983-08-03

    A continuous EtOH fermentor was developed. In the 1st stage of the fermentor, EtOH fermentation medium is contacted with an EtOH-producing bacterium (e.g. Zymomonas mobilis) attached to a carrier material (e.g., vermiculite powder) and with brewers' bottom yeast in the 2nd stage. This system does not require any special cell separator for continuous operation.

  12. Continuous Time Model Estimation

    OpenAIRE

    Carl Chiarella; Shenhuai Gao

    2004-01-01

    This paper introduces an easy to follow method for continuous time model estimation. It serves as an introduction on how to convert a state space model from continuous time to discrete time, how to decompose a hybrid stochastic model into a trend model plus a noise model, how to estimate the trend model by simulation, and how to calculate standard errors from estimation of the noise model. It also discusses the numerical difficulties involved in discrete time models that bring about the unit ...

  13. Continuous parallel coordinates.

    Science.gov (United States)

    Heinrich, Julian; Weiskopf, Daniel

    2009-01-01

    Typical scientific data is represented on a grid with appropriate interpolation or approximation schemes,defined on a continuous domain. The visualization of such data in parallel coordinates may reveal patterns latently contained in the data and thus can improve the understanding of multidimensional relations. In this paper, we adopt the concept of continuous scatterplots for the visualization of spatially continuous input data to derive a density model for parallel coordinates. Based on the point-line duality between scatterplots and parallel coordinates, we propose a mathematical model that maps density from a continuous scatterplot to parallel coordinates and present different algorithms for both numerical and analytical computation of the resulting density field. In addition, we show how the 2-D model can be used to successively construct continuous parallel coordinates with an arbitrary number of dimensions. Since continuous parallel coordinates interpolate data values within grid cells, a scalable and dense visualization is achieved, which will be demonstrated for typical multi-variate scientific data.

  14. Nucleostemin knocking-down causes cell cycle arrest and apoptosis in human T-cell acute lymphoblastic leukemia MOLT-4 cells via p53 and p21Waf1/Cip1 up-regulation.

    Science.gov (United States)

    Rahmati, Marveh; Moosavi, Mohammad Amin; Zarghami, Nosratollah

    2014-12-01

    Nucleostemin (NS), a recently discovered nucleolar protein, is essential for maintaining self-renewal and proliferation of embryonic and adult stem cells as well as cancerous cells. The aim of this study was to determine biological function of NS in MOLT-4 cells as a human T-cell acute lymphocytic leukemia (T-ALL) model. Efficacy of a specific small interference RNA on NS depletion was studied by quantitative polymerase chain reaction and western blotting. The growth rate and viability were analyzed by trypan blue exclusion test. Fluorescent microscopy was used for detecting apoptosis. Cell cycle and apoptosis were mechanistically studied by flow cytometry and western blotting. Knockdown of NS inhibited proliferation, arrested the cell cycle, and induced apoptosis through p53 and p21(Waf1/Cip1) pathways in MOLT-4 cells. These findings demonstrate critical roles of NS in MOLT-4 cells and may implicate on its therapeutic potential in this human T-ALL model.

  15. Gene expression of the p16(INK4a)-Rb and p19(Arf)-p53-p21(Cip/Waf1) signaling pathways in the regulation of hematopoietic stem cell aging by ginsenoside Rg1.

    Science.gov (United States)

    Yue, Z; Rong, J; Ping, W; Bing, Y; Xin, Y; Feng, L D; Yaping, W

    2014-12-04

    The elucidation of the molecular mechanisms underlying the effects of traditional Chinese medicines in clinical practice is a key step toward their worldwide application, and this topic is currently a subject of intense research interest. Rg1, a component of ginsenoside, has recently been shown to perform several pharmacological functions; however, the underlying mechanisms of these effects remain unclear. In the present study, we investigated whether Rg1 has an anti-senescence effect on hematopoietic stem cells (HSCs) and the possible molecular mechanisms driving any effects. The results showed that Rg1 could effectively delay tert-butyl hydroperoxide (t-BHP)-induced senescence and inhibit gene expression in the p16(INK4a)-Rb and p19(Arf)-p53-p21(Cip/Waf1) signaling pathways in HSCs. Our study suggested that these two signaling pathways might be potential targets for elucidating the molecular mechanisms of the Rg1 anti-senescence effect.

  16. Apigenin causes G(2)/M arrest associated with the modulation of p21(Cip1) and Cdc2 and activates p53-dependent apoptosis pathway in human breast cancer SK-BR-3 cells.

    Science.gov (United States)

    Choi, Eun Jeong; Kim, Gun-Hee

    2009-04-01

    We studied the effects of apigenin on the cell cycle distribution and apoptosis of human breast cancer cells and explored the mechanisms underlying these effects. We first investigated the antiproliferative effects in SK-BR-3 cells exposed to between 1 and 100 microM apigenin for 24, 48 and 72 h. Apigenin significantly inhibited cell proliferation at concentrations over 50 microM, regardless of exposure time (P<.05), and resulted in significant cell cycle arrest in the G(2)/M phase after 48 h of treatment at high concentrations (50 and 100 microM; P<.05). To investigate the regulatory proteins of cell cycle arrest affected by apigenin, we treated cells with 50 and 100 microM apigenin for 72 h. Apigenin caused a slight decrease in cyclin D and cyclin E expression, with no change in CDK2 and CDK4. In addition, the apigenin-induced accumulation of the cell population in the G(2)/M phase resulted in a decrease in CDK1 together with cyclin A and cyclin B. In an additional study, apigenin also increased the accumulation of p53 and further enhanced the level of p21(Cip1), with no change in p27(Kip1). The expression of Bax and cytochrome c of p53 downstream target was increased markedly at high concentration treatment over 50 microM apigenin. Based on our findings, the mechanism by which apigenin causes cell cycle arrest via the regulation of CDK1 and p21(Cip1) and induction of apoptosis seems to be involved in the p53-dependent pathway.

  17. 8-60hIPP5(m)-induced G2/M cell cycle arrest involves activation of ATM/p53/p21(cip1/waf1) pathways and delayed cyclin B1 nuclear translocation.

    Science.gov (United States)

    Zeng, Qi-Yan; Zeng, Lin-Jie; Huang, Yu; Huang, Yong-Qi; Zhu, Qi-Fang; Liao, Zhi-Hong

    2014-01-01

    Protein phosphatase 1 (PP1) is a major serine/threonine phosphatase that controls gene expression and cell cycle progression. The active mutant IPP5 (8-60hIPP5(m)), the latest member of the inhibitory molecules for PP1, has been shown to inhibit the growth of human cervix carcinoma cells (HeLa). In order to elucidate the underlying mechanisms, the present study assessed overexpression of 8-60hIPP5(m) in HeLa cells. Flow cytometric and biochemical analyses showed that overexpression of 8-60hIPP5(m) induced G2/M-phase arrest, which was accompanied by the upregulation of cyclin B1 and phosphorylation of G2/M-phase proteins ATM, p53, p21(cip1/waf1) and Cdc2, suggesting that 8-60hIPP5(m) induces G2/M arrest through activation of the ATM/p53/p21(cip1/waf1)/Cdc2/ cyclin B1 pathways. We further showed that overexpression of 8-60hIPP5(m) led to delayed nuclear translocation of cyclin B1. 8-60hIPP5(m) also could translocate to the nucleus in G2/M phase and interact with pp1α and Cdc2 as demonstrated by co-precipitation assay. Taken together, our data demonstrate a novel role for 8-60hIPP5(m) in regulation of cell cycle in HeLa cells, possibly contributing to the development of new therapeutic strategies for cervix carcinoma.

  18. Introduction to Continuous Optimization

    DEFF Research Database (Denmark)

    Andreasson, Niclas; Evgrafov, Anton; Patriksson, Michael

    optimal solutions for continuous optimization models. The main part of the mathematical material therefore concerns the analysis and linear algebra that underlie the workings of convexity and duality, and necessary/sufficient local/global optimality conditions for continuous optimization problems. Natural...... algorithms are then developed from these optimality conditions, and their most important convergence characteristics are analyzed. The book answers many more questions of the form “Why?” and “Why not?” than “How?”. We use only elementary mathematics in the development of the book, yet are rigorous throughout...

  19. Continuous Platform Development

    DEFF Research Database (Denmark)

    Nielsen, Ole Fiil

    low risks and investments but also with relatively fuzzy results. When looking for new platform projects, it is important to make sure that the company and market is ready for the introduction of platforms, and to make sure that people from marketing and sales, product development, and downstream......, but continuous product family evolution challenges this strategy. The concept of continuous platform development is based on the fact that platform development should not be a one-time experience but rather an ongoing process of developing new platforms and updating existing ones, so that product family...

  20. 2015 Workshop on Continuations

    DEFF Research Database (Denmark)

    his volume contains the papers presented at WoC 2015, the Workshop on Continuations held at ETAPS 2015. There were four submissions. Each of them was reviewed by, on the average, three PC members. The committee decided to accept three papers. The program also includes one invited talk. It also...... documents the depth, variety, and richness of continuations with four distilled tutorials. Thanks are due to the local organizers of ETAPS 2015 for the infras- tructure and to the general chairman of WoC 2015, Ugo de'Liguoro, for initiating this workshop and making it happen...

  1. Continuous Reinforced Concrete Beams

    DEFF Research Database (Denmark)

    Hoang, Cao Linh; Nielsen, Mogens Peter

    1996-01-01

    This report deals with stress and stiffness estimates of continuous reinforced concrete beams with different stiffnesses for negative and positive moments e.g. corresponding to different reinforcement areas in top and bottom. Such conditions are often met in practice.The moment distribution...

  2. Continuous Personal Improvement.

    Science.gov (United States)

    Emiliani, M. L.

    1998-01-01

    Suggests that continuous improvement tools used in the workplace can be applied to self-improvement. Explains the use of such techniques as one-piece flow, kanban, visual controls, and total productive maintenance. Points out misapplications of these tools and describes the use of fishbone diagrams to diagnose problems. (SK)

  3. Reparametrizations of Continuous Paths

    DEFF Research Database (Denmark)

    Fahrenberg, Uli; Raussen, Martin

    2007-01-01

    compare it to the distributive lattice of countable subsets of the unit interval. The results obtained are used to analyse the space of traces in a topological space, i.e., the space of continuous paths up to reparametrization equivalence. This space is shown to be homeomorphic to the space of regular...

  4. Promoting Continuing Education Programs.

    Science.gov (United States)

    Hendrickson, Gayle A.

    This handbook is intended for use by institutions in marketing their continuing education programs. A section on "Devising Your Strategy" looks at identifying a target audience, determining the marketing approach, and developing a marketing plan and promotional techniques. A discussion of media options looks at the advantages and…

  5. Continuous venovenous haemodialysis

    DEFF Research Database (Denmark)

    Bistrup, C; Pedersen, R S; Jensen, Dorte Møller

    1996-01-01

    . Standard solutions for peritoneal dialysis are administered in a single-pass manner countercurrent to the blood flow. To control the dialysate flow through the filter, two separate pumps designed for intravenous infusion are used. Anticoagulation is achieved by means of continuous heparin infusion...

  6. Continuing professional development

    NARCIS (Netherlands)

    Collin, K.; Heijden, van der B.I.J.M.; Lewis, P.

    2012-01-01

    Continuing professional development (CPD), when provided formally, is something that is easy to recognize but perhaps rather more difficult to define. Theoretical and empirical controversy surrounds the scope and understanding of the concept. Definition is made more elusive by the different conceptu

  7. Continuous Personal Improvement.

    Science.gov (United States)

    Emiliani, M. L.

    1998-01-01

    Suggests that continuous improvement tools used in the workplace can be applied to self-improvement. Explains the use of such techniques as one-piece flow, kanban, visual controls, and total productive maintenance. Points out misapplications of these tools and describes the use of fishbone diagrams to diagnose problems. (SK)

  8. Continuous coal processing method

    Science.gov (United States)

    Ryason, P. R.

    1980-06-01

    A coal pump is provided in which solid coal is heated in the barrel of an extruder under pressure to a temperature at which the coal assumes plastic properties. The coal is continuously extruded, without static zones, using, for example, screw extrusion preferably without venting through a reduced diameter die to form a dispersed spray. As a result, the dispersed coal may be continuously injected into vessels or combustors at any pressure up to the maximum pressure developed in the extrusion device. The coal may be premixed with other materials such as desulfurization aids or reducible metal ores so that reactions occur, during or after conversion to its plastic state. Alternatively, the coal may be processed and caused to react after extrusion, through the die, with, for example, liquid oxidizers, whereby a coal reactor is provided.

  9. Continuous Fiber Ceramic Composites

    Energy Technology Data Exchange (ETDEWEB)

    None

    2002-09-01

    Fiber-reinforced ceramic composites demonstrate the high-temperature stability of ceramics--with an increased fracture toughness resulting from the fiber reinforcement of the composite. The material optimization performed under the continuous fiber ceramic composites (CFCC) included a series of systematic optimizations. The overall goals were to define the processing window, to increase the robustinous of the process, to increase process yield while reducing costs, and to define the complexity of parts that could be fabricated.

  10. Safety Campaign Continues

    CERN Multimedia

    2002-01-01

    If you see this poster, stop and read it! This is the third poster produced by TIS Division as part of its information campaign on health and safety in the workplace. It provides statistics on occupational accidents at CERN. You will see that, as in the rest of Europe, falls, slips and trips continue to be the main cause of accident. So, eyes open and take care! For more information : http://safety.cern.ch/

  11. 2010 Mississippi Curriculum Framework: Postsecondary Health-Care Assistant. (Program CIP-51.3902 - Nurse/Nursing Assistant/Aide and Client Care Assistant)

    Science.gov (United States)

    Jackson, Jennifer; Sims, Addie

    2010-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  12. 2005 Mississippi Curriculum Framework: Postsecondary Health Care Assistant. (Program CIP: 51.1614 - Nurse/Nursing Assistant/Aide and Client Care Assistant)

    Science.gov (United States)

    Sims, Addie; Burch, Brenda; River, Jayne; Hull, Jennifer; Williams, Judy; Cross, Ella; Lowe, Pam

    2005-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  13. 2010 Mississippi Curriculum Framework: Postsecondary Veterinary Technology. (Program CIP: 51.0808 - Veterinary/Animal Health Technology/Technician and Veterinary Assistant)

    Science.gov (United States)

    Glenn, Bobby; Sills, Kirby

    2010-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  14. Continuous Integration in CFMGR

    CERN Document Server

    Frohlingsdorf, David

    2017-01-01

    Cfmgr is a managing tool for network devices. At the moment there is no way to automatically check the working behaviour of the tool, meaning that a lot of effort is spend into manually testing the tool after an update. During my stay at CERN I developed a black-box testing framework for Cfmgr according to Continuous Integration practices and successfully deployed the framework using Jenkins and Docker. This report discusses in detail how the framework works and how it can be configured, and equally gives a broad problem description and outlines future work directions.

  15. Ethanol by continuous fermentation

    Energy Technology Data Exchange (ETDEWEB)

    1985-01-01

    EtOH is produced by continuous fermentation of molasses. Thus, molasses diluted to 20/sup 0/ Brix was inoculated with bakers' yeast and fermented at 32/sup 0/. When the medium reached 4% EtOH, it was circulated through a centrifuge. The concentrated yeast was recycled to the fermentor and the supernatant was sent to a still. After distillation, the still residue was returned to the fermentor. When the initial charge was entirely fermented, 13 kg molasses of 40/sup 0/ Brix was added to the fermentor per hour, and the yield of EtOH was 1.7 kg/100 L-hour.

  16. Continuous Reinforced Concrete Beams

    DEFF Research Database (Denmark)

    Hoang, Cao Linh; Nielsen, Mogens Peter

    1996-01-01

    This report deals with stress and stiffness estimates of continuous reinforced concrete beams with different stiffnesses for negative and positive moments e.g. corresponding to different reinforcement areas in top and bottom. Such conditions are often met in practice.The moment distribution...... at the limit state of serviceability is in some simple cases determined by setting up the statical and the compatibility conditions.With these moment distributions, the maximum deflection and the reinforcement stresses at the span middle and at a support are calculated.The results are compared with results...

  17. Continuous Shearlet Tight Frames

    KAUST Repository

    Grohs, Philipp

    2010-10-22

    Based on the shearlet transform we present a general construction of continuous tight frames for L2(ℝ2) from any sufficiently smooth function with anisotropic moments. This includes for example compactly supported systems, piecewise polynomial systems, or both. From our earlier results in Grohs (Technical report, KAUST, 2009) it follows that these systems enjoy the same desirable approximation properties for directional data as the previous bandlimited and very specific constructions due to Kutyniok and Labate (Trans. Am. Math. Soc. 361:2719-2754, 2009). We also show that the representation formulas we derive are in a sense optimal for the shearlet transform. © 2010 Springer Science+Business Media, LLC.

  18. Continuous system modeling

    Science.gov (United States)

    Cellier, Francois E.

    1991-01-01

    A comprehensive and systematic introduction is presented for the concepts associated with 'modeling', involving the transition from a physical system down to an abstract description of that system in the form of a set of differential and/or difference equations, and basing its treatment of modeling on the mathematics of dynamical systems. Attention is given to the principles of passive electrical circuit modeling, planar mechanical systems modeling, hierarchical modular modeling of continuous systems, and bond-graph modeling. Also discussed are modeling in equilibrium thermodynamics, population dynamics, and system dynamics, inductive reasoning, artificial neural networks, and automated model synthesis.

  19. Continuous steel production and apparatus

    Science.gov (United States)

    Peaslee, Kent D.; Peter, Jorg J.; Robertson, David G. C.; Thomas, Brian G.; Zhang, Lifeng

    2009-11-17

    A process for continuous refining of steel via multiple distinct reaction vessels for melting, oxidation, reduction, and refining for delivery of steel continuously to, for example, a tundish of a continuous caster system, and associated apparatus.

  20. Continuously adjustable Pulfrich spectacles

    Science.gov (United States)

    Jacobs, Ken; Karpf, Ron

    2011-03-01

    A number of Pulfrich 3-D movies and TV shows have been produced, but the standard implementation has inherent drawbacks. The movie and TV industries have correctly concluded that the standard Pulfrich 3-D implementation is not a useful 3-D technique. Continuously Adjustable Pulfrich Spectacles (CAPS) is a new implementation of the Pulfrich effect that allows any scene containing movement in a standard 2-D movie, which are most scenes, to be optionally viewed in 3-D using inexpensive viewing specs. Recent scientific results in the fields of human perception, optoelectronics, video compression and video format conversion are translated into a new implementation of Pulfrich 3- D. CAPS uses these results to continuously adjust to the movie so that the viewing spectacles always conform to the optical density that optimizes the Pulfrich stereoscopic illusion. CAPS instantly provides 3-D immersion to any moving scene in any 2-D movie. Without the glasses, the movie will appear as a normal 2-D image. CAPS work on any viewing device, and with any distribution medium. CAPS is appropriate for viewing Internet streamed movies in 3-D.

  1. Continuing medical education.

    Science.gov (United States)

    Todd, D

    1987-04-01

    With the rapid advances in medical science and increasing complexities of patient care, the need for continuing medical education (CME) is widely accepted by the profession. CME follows general and higher professional training, and should be a life long process. Teaching hospitals and postgraduate professional institutions play vital roles in organising, promoting, and monitoring this activity. CME directorates should be established. University authorities must recognise the important role of medical teachers in postgraduate and continuing medical education, and the staff establishment and terms of service should be held regularly. Medical libraries should have easy borrowing facilities. Self-assessment and audio-visual material are particularly helpful to the busy practitioner and inexpensive local or regional journals of quality can provide pertinent and up-to-date information. All charges for attending scientific meetings and educational material should be tax deductible or subsidized. The effectiveness of CME is difficult to assess and participation is almost impossible to enforce. Much depends on the standard of medical practice wanted by society. Recertification of general practitioners or specialists poses many problems. On the other hand, completion of self-assessment programmes, active participation at medical meetings, contributions to scientific literature, and membership of medical societies with built-in peer review could be monitored and regularly used to evaluate professional status.

  2. Landsat Data Continuity Mission

    Science.gov (United States)

    ,

    2012-01-01

    The Landsat Data Continuity Mission (LDCM) is a partnership formed between the National Aeronautics and Space Administration (NASA) and the U.S. Geological Survey (USGS) to place the next Landsat satellite in orbit in January 2013. The Landsat era that began in 1972 will become a nearly 41-year global land record with the successful launch and operation of the LDCM. The LDCM will continue the acquisition, archiving, and distribution of multispectral imagery affording global, synoptic, and repetitive coverage of the Earth's land surfaces at a scale where natural and human-induced changes can be detected, differentiated, characterized, and monitored over time. The mission objectives of the LDCM are to (1) collect and archive medium resolution (30-meter spatial resolution) multispectral image data affording seasonal coverage of the global landmasses for a period of no less than 5 years; (2) ensure that LDCM data are sufficiently consistent with data from the earlier Landsat missions in terms of acquisition geometry, calibration, coverage characteristics, spectral characteristics, output product quality, and data availability to permit studies of landcover and land-use change over time; and (3) distribute LDCM data products to the general public on a nondiscriminatory basis at no cost to the user.

  3. Tongue epithelial KT-1 cell-cycle arrest by TGF-β associated with induction of p21Cip1 and p15Ink4b

    OpenAIRE

    Nakamura, Shin-ichi; Kamakura, Takashi; Ookura, Tetsuya

    2009-01-01

    Tongue epithelium continuously turns over in adults. Our previous study showed that epidermal growth factor and fibroblast growth factor-2 stimulated proliferation of KT-1 cells derived from tongue epithelium, suggesting that these signals serve as positive regulators for tongue epithelial proliferation. To investigate a negative regulation of tongue epithelial cell proliferation, we studied effects of transforming growth factor-β (TGF-β) on KT-1 cells. Proliferation assays showed that TGF-β ...

  4. Effect of human papillomavirus on cell cycle-related proteins p16INK4A, p21waf1/cip1, p53 and cyclin D1 in sinonasal inverted papilloma and laryngeal carcinoma. An in situ hybridization study

    Directory of Open Access Journals (Sweden)

    Marian Danilewicz

    2011-04-01

    Full Text Available Human papillomavirus (HPV infection is implicated as an important risk factor in the development of head and neck cancers. Many studies focusing on the relationships between HPV infection and cell cycle proteins immunoexpression in laryngeal lesions have provided contradictory results. The aim of this study was to evaluate the relationships between HPV DNA presence and p16INK4a, p21waf1/cip1, p53 and cyclin D1 immunoexpression in heterogenous HPV-positive and HPV-negative groups of laryngeal cancers and inverted papillomas. The HPV DNA expression was detected using an in situ hybridization method and immunoexpression of p16INK4a, p21waf1/cip1, p53 and cyclin D1 using immunohistochemistry. The immunoexpression of p21waf1/ /cip1 and p53 proteins was lower in the HPV-positive group compared to the HPV-negative group, although only the difference of p53 staining was statistically significant. The immunoexpression of p16INK4a and cyclin D1 was significantly increased in the HPV-positive group compared to the HPV-negative group. The increased immunoexpression of p16INK4a and cyclin D1, and the lower immunoexpression of p21waf1/cip1 and p53 in the HPV-positive group compared to the HPV-negative group, supports the hypothesis that HPV may play an important role in cell cycle dysregulation. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 1, pp. 34–40

  5. Quasi-continuous magnets

    Energy Technology Data Exchange (ETDEWEB)

    Sims, J.R. [Los Alamos National Lab., NM (United States); Naumovich, G.J.; Hoang, T.A.; Dent, P.C. [Everson Electric Co., Bethlehem, PA (United States)

    1996-05-01

    The National High Magnetic Field Laboratory is completing a quasi-continuous magnet which will sustain a constant field of 60 T for 100 ms in a 32-mm 77 K bore. This magnet consists of 9 mechanically independent, nested, liquid nitrogen-cooled coils which are individually reinforced by high-strength stainless steel outer shells. The coils were wound from rectangular large cross-section, high-strength, high-conductivity copper conductor insulated wtih polyimide and fiberglass tapes. After winding, the coils were inserted into closely fitted, stainless steel reinforcing shells and impregnated with epoxy resin. Design, analysis, material, fabrication and operational issues for this class of magnets are reviewed. Fabrication and quality assurance testing of the 60 T coil set are covered in detail. Future growth of and possible links from this technology to other magnet systems are discussed. Needed improvements in design, analysis, materials, and fabrication are outlined.

  6. [Continuous-infusion ketamine].

    Science.gov (United States)

    Mancini, P G; Caggese, G; Di Fabio, A; Di Nino, G F; Cocchi, V

    1980-08-01

    An investigation was made of the employment of ketamin as the sole anaesthetic in general surgery, using continuous infusion of a 1% solution for both induction and maintenance in 118 cases. ECG was monitored and arterial pressure was measured invasively. Central venous pressure was also determined in 10 cases. Changes in serum enzyme values during and after surgery were examined in 35 patients. Blood samples were withdrawn before induction, after the return to consciousness, and 24 hr after the operation. Side-effects were common, but slight. Five patients suffered from nightmares, but these were persons with marked imaginative activity and a melancholic nature. Cardiocirculatory function was satisfactory. In particular, peripheral perfusion was excellent in all cases.

  7. Knowledge and Continuous Innovation

    DEFF Research Database (Denmark)

    Boer, Harry; Caffyn, S.; Corso, M.

    1999-01-01

    proposes a model to describe and explain how companies can gain a substantive competitive advantage by extending their innovation efforts to other phases of the product life cycle and by facilitating knowledge transfer and learning both within the company and with other partner organisations. The model......Competition today is forcing companies to increase their effectiveness through exploiting synergy and learning in product innovation. Literature, however, is still mainly focused on how product development projects, seen largely as isolated efforts, should be organised and managed. This article...... is based on collaborative research by the authors, based on their involvement in the Euro-Australian co-operation project CIMA (Euro-Australian co-operation centre for Continuous Improvement and innovation MAnagement)....

  8. Spaces of continuous functions

    CERN Document Server

    Groenewegen, G L M

    2016-01-01

    The space C(X) of all continuous functions on a compact space X carries the structure of a normed vector space, an algebra and a lattice. On the one hand we study the relations between these structures and the topology of X, on the other hand we discuss a number of classical results according to which an algebra or a vector lattice can be represented as a C(X). Various applications of these theorems are given. Some attention is devoted to related theorems, e.g. the Stone Theorem for Boolean algebras and the Riesz Representation Theorem. The book is functional analytic in character. It does not presuppose much knowledge of functional analysis; it contains introductions into subjects such as the weak topology, vector lattices and (some) integration theory.

  9. Continuous Culture Microbioreactors

    DEFF Research Database (Denmark)

    Schäpper, Daniel

    . The advance of miniature online measuring techniques makes it possible to measure at least the basic culture variables such as dissolved oxygen (DO), cell density (OD) and pH continuously and without disturbing the cultivation. Online measurements are at this scale very susceptible to the presence of bubbles...... focus was put on the single-use aspect. This includes both being cheap in fabrication and in operation, and also requires the reactor to be sterilizable by industrial methods. It consists entirely of polydimethylsiloxane (PDMS) and contains two optical sensor spots for the measurement of DO and p.......2 mm height. The reactor chamber is a cylinder with 8 mm diameter and 2 mm height resulting in a culture volume of 100 L. The uidic connections are done by piercing the reactor side walls with needles|the PDMS will tightly enclose the needle to prevent leakage. The reactor chamber is sealed with a semi...

  10. Pipeline External Corrosion Direct Assessment (ECDA) Close Interval Potential Survey (CIPS) Application of Detection Method%钢质管道外腐蚀直接评价(ECDA)过程中密间隔电位(CIPS)检测方法的应用

    Institute of Scientific and Technical Information of China (English)

    袁昕; 朱小铁

    2015-01-01

    This paper introduces the basic principle of CIPS close interval potential measurements and 5 kinds of detection model and its application, and through a successful implementation of CIPS close interval measuring, derive the analytical detection data on / off potential, horizontal / vertical potential gradient in the pipeline anticorrosion layer damage of different data distribution points, by the law the method can be implemented close interval potential using CIPS measurements, breakage point location of the potential and the pipeline anticorrosion layer pipeline cathodic protection.%本文介绍了CIPS密间隔电位测量的基本原理和5种检测模式及其应用情况,并通过一次成功的实施CIPS密间隔电位测量,解析检测数据的得出通/断电电位、横/纵向电位梯度在管道防腐层破损点处的不同数据分布规律,通过该规律可以实现使用CIPS密间隔电位测量的方法,对管道阴极保护电位和管道防腐层破损点的定位。

  11. Damage Detection of a Continuous Bridge from Response of a Moving Vehicle

    Directory of Open Access Journals (Sweden)

    Z. H. Li

    2014-01-01

    Full Text Available This paper presents a multistage multipass method to identify the damage location of a continuous bridge from the response of a vehicle moving on the rough road surface of the bridge. The vehicle runs over the bridge several times at different velocities and the corresponding responses of the vehicle can be obtained. The vertical accelerations of the vehicle running on the intact and damaged bridges are used for identification. The multistage damage detection method is implemented by the modal strain energy based method and genetic algorithm. The modal strain energy based method estimates the damage location by calculating a damage indicator from the frequencies extracted from the vehicle responses of both the intact and damaged states of the bridge. At the second stage, the identification problem is transformed into a global optimization problem and is solved by genetic algorithm techniques. For each pass of the vehicle, the method can identify the location of the damage until it is determined with acceptable accuracy. A two-span continuous bridge is used to verify the method. The numerical results show that this method can identify the location of damage reasonably well.

  12. MST kinases monitor actin cytoskeletal integrity and signal via c-Jun N-terminal kinase stress-activated kinase to regulate p21Waf1/Cip1 stability.

    Science.gov (United States)

    Densham, Ruth M; O'Neill, Eric; Munro, June; König, Ireen; Anderson, Kurt; Kolch, Walter; Olson, Michael F

    2009-12-01

    As well as providing a structural framework, the actin cytoskeleton plays integral roles in cell death, survival, and proliferation. The disruption of the actin cytoskeleton results in the activation of the c-Jun N-terminal kinase (JNK) stress-activated protein kinase (SAPK) pathway; however, the sensor of actin integrity that couples to the JNK pathway has not been characterized in mammalian cells. We now report that the mammalian Ste20-like (MST) kinases mediate the activation of the JNK pathway in response to the disruption of the actin cytoskeleton. One consequence of actin disruption is the JNK-mediated stabilization of p21(Waf1/Cip1) (p21) via the phosphorylation of Thr57. The expression of MST1 or MST2 was sufficient to stabilize p21 in a JNK- and Thr57-dependent manner, while the stabilization of p21 by actin disruption required MST activity. These data indicate that, in addition to being components of the Salvador-Warts-Hippo tumor suppressor network and binding partners of c-Raf and the RASSF1A tumor suppressor, MST kinases serve to monitor cytoskeletal integrity and couple via the JNK SAPK pathway to the regulation of a key cell cycle regulatory protein.

  13. Estudo comparativo da eficácia de sanitizantes por meio da técnica de ATP - bioluminescência em sistemas de higienização do tipo clean-in-place (CIP

    Directory of Open Access Journals (Sweden)

    Adbeel de Lima Santos

    2009-10-01

    Full Text Available Utilizou-se o método de ATP-bioluminescência para avaliação de procedimentos de higienização CIP, com diferentes sanitizantes, de um homogeneizador em uma indústria de laticínios de Juiz de Fora - MG. A eficácia do processo foi comparada com base na utilização de dióxido de cloro estabilizado em solução aquosa, hipoclorito de sódio, ácido peracético e iodóforo. Os resultados obtidos permitiram concluir que o dióxido de cloro e o ácido peracético possuem a mesma eficácia. O mesmo ocorre com o hipoclorito de sódio e o iodóforo, porém, estes últimos não foram eficazes nas condições analisadas.

  14. A new species of Atractus (Serpentes: Dipsadidae from Serra do Cipó, Espinhaço Range, Southeastern Brazil, with proposition of a new species group to the genus

    Directory of Open Access Journals (Sweden)

    Paulo Passos

    2013-01-01

    Full Text Available We describe a new species of Atractus from Serra do Cipó, at the southeastern versant of Serra do Espinhaço, an inland mountain range nearly parallel to the Brazilian Atlantic coast. The new species is morphologically similar to A. paraguayensis and A. potschi with which it shares: 15 dorsal scale rows; white occipital region in juvenile specimens; dorsal ground color reddish pink to red (in life with alternate black transversal spots, blotches or transversal bands; seven upper and lower labial scales; ventrals and subcaudals creamish white; hemipenis slightly bilobed, semicapitate and semicalyculate. The new species differs from both taxa mainly on the basis of exclusive coloration characters (number and shape of transversal bands and hemipenial morphology (median face of the lobes without ornamentation, besides punctual meristic (number of ventral scales and maxillary teeth and morphometric (adult snout-vent length differences. Finally, we provide a discussion regarding the potential affinities of the new species and we redefine some Atractus species groups in order to better accommodate species sharing unique combinations of morphological characters.

  15. Entanglement Continuous Unitary Transformations

    CERN Document Server

    Sahin, S; Orus, R

    2016-01-01

    Continuous unitary transformations are a powerful tool to extract valuable information out of quantum many-body Hamiltonians, in which the so-called flow equation transforms the Hamiltonian to a diagonal or block-diagonal form in second quantization. Yet, one of their main challenges is how to approximate the infinitely-many coupled differential equations that are produced throughout this flow. Here we show that tensor networks offer a natural and non-perturbative truncation scheme in terms of entanglement. The corresponding scheme is called "entanglement-CUT" or eCUT. It can be used to extract the low-energy physics of quantum many-body Hamiltonians, including quasiparticle energy gaps. We provide the general idea behind eCUT and explain its implementation for finite 1d systems using the formalism of matrix product operators, and we present proof-of-principle results for the spin-1/2 1d quantum Ising model in a transverse field. Entanglement-CUTs can also be generalized to higher dimensions and to the thermo...

  16. Continuous home oxygen therapy.

    Science.gov (United States)

    Ortega Ruiz, Francisco; Díaz Lobato, Salvador; Galdiz Iturri, Juan Bautista; García Rio, Francisco; Güell Rous, Rosa; Morante Velez, Fátima; Puente Maestu, Luis; Tàrrega Camarasa, Julia

    2014-05-01

    Oxygen therapy is defined as the therapeutic use of oxygen and consists of administering oxygen at higher concentrations than those found in room air, with the aim of treating or preventing hypoxia. This therapeutic intervention has been shown to increase survival in patients with chronic obstructive pulmonary disease (COPD) and respiratory failure. Although this concept has been extended by analogy to chronic respiratory failure caused by respiratory and non-respiratory diseases, continuous oxygen therapy has not been shown to be effective in other disorders. Oxygen therapy has not been shown to improve survival in patients with COPD and moderate hypoxaemia, nor is there consensus regarding its use during nocturnal desaturations in COPD or desaturations caused by effort. The choice of the oxygen source must be made on the basis of criteria such as technical issues, patient comfort and adaptability and cost. Flow must be adjusted to achieve appropriate transcutaneous oxyhaemoglobin saturation correction. Copyright © 2013 SEPAR. Published by Elsevier Espana. All rights reserved.

  17. Entanglement continuous unitary transformations

    Science.gov (United States)

    Sahin, Serkan; Schmidt, Kai Phillip; Orús, Román

    2017-01-01

    Continuous unitary transformations are a powerful tool to extract valuable information out of quantum many-body Hamiltonians, in which the so-called flow equation transforms the Hamiltonian to a diagonal or block-diagonal form in second quantization. Yet, one of their main challenges is how to approximate the infinitely-many coupled differential equations that are produced throughout this flow. Here we show that tensor networks offer a natural and non-perturbative truncation scheme in terms of entanglement. The corresponding scheme is called “entanglement-CUT” or eCUT. It can be used to extract the low-energy physics of quantum many-body Hamiltonians, including quasiparticle energy gaps. We provide the general idea behind eCUT and explain its implementation for finite 1d systems using the formalism of matrix product operators. We also present proof-of-principle results for the spin-(1/2) 1d quantum Ising model and the 3-state quantum Potts model in a transverse field. Entanglement-CUTs can also be generalized to higher dimensions and to the thermodynamic limit.

  18. Periodic review and continuous ordering

    OpenAIRE

    Prak, Dennis R.J.; Teunter, Ruud; Riezebos, Jan

    2014-01-01

    There exist many inventory control studies that consider either continuous review & continuous ordering, or periodic review & periodic ordering. Mixtures of the two are hardly ever studied. However, the model with periodic review and continuous ordering is highly relevant in practice, as information on the actual inventory level is not always up to date while making ordering decisions. This paper will therefore consider this case of periodic review and continuous ordering. Assuming zero fixed...

  19. Periodic review and continuous ordering

    NARCIS (Netherlands)

    Prak, Dennis; Teunter, Ruud; Riezebos, Jan

    2015-01-01

    Many inventory control studies consider either continuous review and continuous ordering, or periodic review and periodic ordering. Mixtures of the two are hardly ever studied. However, the model with periodic review and continuous ordering is highly relevant in practice, as information on the actua

  20. Periodic review and continuous ordering

    NARCIS (Netherlands)

    Prak, Dennis R.J.; Teunter, Ruud; Riezebos, Jan

    2014-01-01

    There exist many inventory control studies that consider either continuous review & continuous ordering, or periodic review & periodic ordering. Mixtures of the two are hardly ever studied. However, the model with periodic review and continuous ordering is highly relevant in practice, as information

  1. Periodic review and continuous ordering

    NARCIS (Netherlands)

    Prak, Dennis R.J.; Teunter, Ruud; Riezebos, Jan

    2014-01-01

    There exist many inventory control studies that consider either continuous review & continuous ordering, or periodic review & periodic ordering. Mixtures of the two are hardly ever studied. However, the model with periodic review and continuous ordering is highly relevant in practice, as information

  2. Periodic review and continuous ordering

    NARCIS (Netherlands)

    Prak, Dennis; Teunter, Ruud; Riezebos, Jan

    2015-01-01

    Many inventory control studies consider either continuous review and continuous ordering, or periodic review and periodic ordering. Mixtures of the two are hardly ever studied. However, the model with periodic review and continuous ordering is highly relevant in practice, as information on the

  3. Coded continuous wave meteor radar

    OpenAIRE

    2015-01-01

    The concept of coded continuous wave meteor radar is introduced. The radar uses a continuously transmitted pseudo-random waveform, which has several advantages: coding avoids range aliased echoes, which are often seen with commonly used pulsed specular meteor radars (SMRs); continuous transmissions maximize pulse compression gain, allowing operation with significantly lower peak transmit power; the temporal resolution can be changed after ...

  4. DAF-16/FOXO regulates transcription of cki-1/Cip/Kip and repression of lin-4 during C. elegans L1 arrest.

    Science.gov (United States)

    Baugh, L Ryan; Sternberg, Paul W

    2006-04-18

    Development is typically studied as a continuous process under laboratory conditions, but wild animals often develop in variable and stressful environments. C. elegans larvae hatch in a developmentally arrested state (L1 arrest) and initiate post-embryonic development only in the presence of food (E. coli in lab). In contrast to the well-studied dauer arrest, L1 arrest occurs without morphological modification, although larvae in L1 arrest are more resistant to environmental stress than developing larvae . Consistent with its role in dauer formation and aging, we show that insulin/insulin-like growth factor (IGF) signaling regulates L1 arrest. daf-2 insulin/IGF receptor mutants have a constitutive-L1-arrest phenotype when fed and extended survival of L1 arrest when starved. Conversely, daf-16/FOXO mutants have a defective-arrest phenotype, failing to arrest development and dying rapidly when starved. We show that DAF-16 is required for transcription of the cyclin-dependent kinase inhibitor cki-1 in stem cells in response to starvation, accounting for the failure of daf-16/FOXO mutants to arrest cell division during L1 arrest. Other developmental events such as cell migration, cell fusion, and expression of the microRNA lin-4, a temporal regulator of post-embryonic development, are also observed in starved daf-16/FOXO mutants. These results suggest that DAF-16/FOXO promotes developmental arrest via transcriptional regulation of numerous target genes that control various aspects of development.

  5. Exoplanets: The Hunt Continues!

    Science.gov (United States)

    2001-04-01

    Swiss Telescope at La Silla Very Successful Summary The intensive and exciting hunt for planets around other stars ( "exoplanets" ) is continuing with great success in both hemispheres. Today, an international team of astronomers from the Geneva Observatory and other research institutes [1] is announcing the discovery of no less than eleven new, planetary companions to solar-type stars, HD 8574, HD 28185, HD 50554, HD 74156, HD 80606, HD 82943, HD 106252, HD 141937, HD 178911B, HD 141937, among which two new multi-planet systems . The masses of these new objects range from slightly less than to about 10 times the mass of the planet Jupiter [2]. The new detections are based on measured velocity changes of the stars [3], performed with the CORALIE spectrometer on the Swiss 1.2-m Leonard Euler telescope at the ESO La Silla Observatory , as well as with instruments on telescopes at the Haute-Provence Observatory and on the Keck telescopes on Mauna Kea (Hawaii, USA). Some of the new planets are unusual: * a two-planet system (around the star HD 82943) in which one orbital period is nearly exactly twice as long as the other - cases like this (refered to as "orbital resonance") are well known in our own solar system; * another two-planet system (HD 74156), with a Jupiter-like planet and a more massive planet further out; * a planet with the most elongated orbit detected so far (HD 80606), moving between 5 and 127 million kilometers from the central star; * a giant planet moving in an orbit around its Sun-like central star that is very similar to the one of the Earth and whose potential satellites (in theory, at least) might be "habitable". At this moment, there are 63 know exoplanet candidates with minimum masses below 10 Jupiter masses, and 67 known objects with minimum masses below 17 Jupiter masses. The present team of astronomers has detected about half of these. PR Photo 13a/01 : Radial-velocity measurements of HD 82943, a two-planet system . PR Photo 13b/01 : Radial

  6. Continuous downstream processing of biopharmaceuticals.

    Science.gov (United States)

    Jungbauer, Alois

    2013-08-01

    Continuous manufacturing has been applied in many different industries but has been pursued reluctantly in biotechnology where the batchwise process is still the standard. A shift to continuous operation can improve productivity of a process and substantially reduce the footprint. Continuous operation also allows robust purification of labile biomolecules. A full set of unit operations is available to design continuous downstream processing of biopharmaceuticals. Chromatography, the central unit operation, is most advanced in respect to continuous operation. Here, the problem of 'batch' definition has been solved. This has also paved the way for implementation of continuous downstream processing from a regulatory viewpoint. Economic pressure, flexibility, and parametric release considerations will be the driving force to implement continuous manufacturing strategies in future.

  7. Cell-penetrating superoxide dismutase attenuates oxidative stress-induced senescence by regulating the p53-p21Cip1 pathway and restores osteoblastic differentiation in human dental pulp stem cells

    Directory of Open Access Journals (Sweden)

    Park YJ

    2012-09-01

    Full Text Available Yoon Jung Choi,1,* Jue Yeon Lee,2,* Chong Pyoung Chung,2 Yoon Jeong Park,1,21Craniomaxillofacial Reconstructive Sciences, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Republic of Korea; 2Research Institute, Nano Intelligent Biomedical Engineering, Seoul, Republic of Korea*These authors contributed equally to this workBackground: Human dental pulp stem cells (DPSCs have potential applications in tissue regeneration because of their convenient cell harvesting procedures and multipotent capacity. However, the tissue regenerative potential of DPSCs is known to be negatively regulated by aging in long-term culture and under oxidative stress. With an aim of reducing cellular senescence and oxidative stress in DPSCs, an intracellular delivery system for superoxide dismutase 1 (SOD1 was developed. We conjugated SOD1 with a cell-penetrating peptide known as low-molecular weight protamine (LMWP, and investigated the effect of LMWP-SOD1 conjugates on hydrogen peroxide-induced cellular senescence and osteoblastic differentiation.Results: LMWP-SOD1 significantly attenuated enlarged and flattened cell morphology and increased senescence-associated β-galactosidase activity. Under the same conditions, LMWP-SOD1 abolished activation of the cell cycle regulator proteins, p53 and p21Cip1, induced by hydrogen peroxide. In addition, LMWP-SOD1 reversed the inhibition of osteoblastic differentiation and downregulation of osteogenic gene markers induced by hydrogen peroxide. However, LMWP-SOD1 could not reverse the decrease in odontogenesis caused by hydrogen peroxide.Conclusion: Overall, cell-penetrating LMWP-SOD1 conjugates are effective for attenuation of cellular senescence and reversal of osteoblastic differentiation of DPSCs caused by oxidative stress inhibition. This result suggests potential application in the field of antiaging and tissue engineering to overcome the limitations of senescent stem cells.Keywords: superoxide

  8. CDK5 Regulates Paclitaxel Sensitivity in Ovarian Cancer Cells by Modulating AKT Activation, p21Cip1- and p27Kip1-Mediated G1 Cell Cycle Arrest and Apoptosis.

    Directory of Open Access Journals (Sweden)

    Shu Zhang

    Full Text Available Cyclin-dependent kinase 5 (CDK5 is a cytoplasmic serine/ threonine kinase. Knockdown of CDK5 enhances paclitaxel sensitivity in human ovarian cancer cells. This study explores the mechanisms by which CDK5 regulates paclitaxel sensitivity in human ovarian cancers. Multiple ovarian cancer cell lines and xenografts were treated with CDK5 small interfering RNA (siRNA with or without paclitaxel to examine the effect on cancer cell viability, cell cycle arrest and tumor growth. CDK5 protein was measured by immunohistochemical staining of an ovarian cancer tissue microarray to correlate CDK5 expression with overall patient survival. Knockdown of CDK5 with siRNAs inhibits activation of AKT which significantly correlates with decreased cell growth and enhanced paclitaxel sensitivity in ovarian cancer cell lines. In addition, CDK5 knockdown alone and in combination with paclitaxel induced G1 cell cycle arrest and caspase 3 dependent apoptotic cell death associated with post-translational upregulation and nuclear translocation of TP53 and p27(Kip1 as well as TP53-dependent transcriptional induction of p21(Cip1 in wild type TP53 cancer cells. Treatment of HEYA8 and A2780 wild type TP53 xenografts in nu/nu mice with CDK5 siRNA and paclitaxel produced significantly greater growth inhibition than either treatment alone. Increased expression of CDK5 in human ovarian cancers correlates inversely with overall survival. CDK5 modulates paclitaxel sensitivity by regulating AKT activation, the cell cycle and caspase-dependent apoptosis. CDK5 inhibition can potentiate paclitaxel activity in human ovarian cancer cells.

  9. Inactivation of p16INK4a, with retention of pRB and p53/p21cip1 function, in human MRC5 fibroblasts that overcome a telomere-independent crisis during immortalization.

    Science.gov (United States)

    Taylor, Lisa M; James, Alexander; Schuller, Christine E; Brce, Jesena; Lock, Richard B; Mackenzie, Karen L

    2004-10-15

    Recent investigations, including our own, have shown that specific strains of fibroblasts expressing telomerase reverse transcriptase (hTERT) have an extended lifespan, but are not immortal. We previously demonstrated that hTERT-transduced MRC5 fetal lung fibroblasts (MRC5hTERTs) bypassed senescence but eventually succumbed to a second mortality barrier (crisis). In the present study, 67 MRC5hTERT clones were established by limiting dilution of a mass culture. Whereas 39/67 clones had an extended lifespan, all 39 extended lifespan clones underwent crisis. 11 of 39 clones escaped crisis and were immortalized. There was no apparent relationship between the fate of clones at crisis and the level of telomerase activity. Telomeres were hyperextended in the majority of the clones analyzed. There was no difference in telomere length of pre-crisis compared with post-crisis and immortal clones, indicating that hyperextended telomeres were conducive for immortalization and confirming that crisis was independent of telomere length. Immortalization of MRC5hTERT cells was associated with repression of the cyclin-dependent kinase inhibitor p16INK4a and up-regulation of pRB. However, the regulation of pRB phosphorylation and the response of the p53/p21cip1/waf1 pathway were normal in immortal cells subject to genotoxic stress. Overexpression of oncogenic ras failed to de-repress p16INK4a in immortal cells. Furthermore, expression of ras enforced senescent-like growth arrest in p16INK4a-positive, but not p16INK4a-negative MRC5hTERT cells. Immortal cells expressing ras formed small, infrequent colonies in soft agarose, but were non-tumorigenic. Overall, these results implicate the inactivation of p16INK4a as a critical event for overcoming telomere-independent crisis, immortalizing MRC5 fibroblasts and overcoming ras-induced premature senescence.

  10. Estudo de p27, p21, p16 em epitélio escamoso normal, papiloma escamoso e carcinoma de células escamosas da cavidade oral Comparative analysis of the immunohistochemistry expression of p27, p21WAF/Cip1, and p16INK4a in oral normal epithelium, squamous papilloma and squamous cell carcinoma

    Directory of Open Access Journals (Sweden)

    Ana Beatriz Piazza Queiroz

    2009-12-01

    Full Text Available INTRODUÇÃO E OBJETIVO: O tipo de câncer oral mais frequente é o carcinoma de células escamosas, que corresponde a 95% dos casos(9. O papiloma escamoso oral é uma neoplasia benigna normalmente associada à infecção pelo papilomavírus humano (HPV(21. A análise da literatura mostra alterações nos genes reguladores do ciclo celular p27, p21WAF/Cip1 e p16INK4a, porém sem uma definição de seus papéis na carcinogênese oral. O objetivo foi caracterizar imuno-histoquimicamente p27, p21WAF/Cip1 e p16NK4a em epitélio escamoso normal, papilomas escamosos e carcinomas de células escamosas da cavidade oral. MÉTODOS: Imuno-histoquímica para p27, p21WAF/Cip1 e p16NK4a em 32 casos de epitélio escamoso normal, 30 casos de papiloma escamoso e 34 de carcinoma de células escamosas da cavidade oral. RESULTADOS: p27: 97,06% dos casos de carcinoma de células escamosas apresentaram imunopositividade focal. O grupo papiloma escamoso apresentou 33,33% e o grupo controle, 18,75%. p21WAF/Cip1: 100% de imunopositividade focal tanto no grupo controle como no grupo carcinoma de células escamosas, e 90% no grupo papiloma escamoso. p16INK4a: 100% de imunopositividade focal para os grupos controle e papiloma escamoso, e 94% para o grupo carcinoma de células escamosas. CONCLUSÃO: Imuno-histoquimicamente demonstrou-se diferença significativa para p27 quando feita comparação dos grupos controle e papiloma escamoso com o grupo carcinoma de células escamosas. O p21WAF/Cip1 não demonstrou poder de diferenciar os grupos analisados. O p16INK4a apresentou imunopositividade difusa em uma minoria dos casos do grupo carcinoma de células escamosas. O grupo papiloma escamoso se comportou de maneira similar ao grupo controle em relação aos três marcadores.INTRODUCTION: The most frequent type of oral cancer is the squamous cell carcinoma, which corresponds to 95% of the cases(9.The oral squamous papilloma is a benign neoplasia, commonly associated with

  11. Continuous verification using multimodal biometrics.

    Science.gov (United States)

    Sim, Terence; Zhang, Sheng; Janakiraman, Rajkumar; Kumar, Sandeep

    2007-04-01

    Conventional verification systems, such as those controlling access to a secure room, do not usually require the user to reauthenticate himself for continued access to the protected resource. This may not be sufficient for high-security environments in which the protected resource needs to be continuously monitored for unauthorized use. In such cases, continuous verification is needed. In this paper, we present the theory, architecture, implementation, and performance of a multimodal biometrics verification system that continuously verifies the presence of a logged-in user. Two modalities are currently used--face and fingerprint--but our theory can be readily extended to include more modalities. We show that continuous verification imposes additional requirements on multimodal fusion when compared to conventional verification systems. We also argue that the usual performance metrics of false accept and false reject rates are insufficient yardsticks for continuous verification and propose new metrics against which we benchmark our system.

  12. CONTINUITY OF DYNAMIC-SYSTEMS - THE CONTINUOUS-TIME CASE

    NARCIS (Netherlands)

    NIEUWENHUIS, JW; WILLEMS, JC

    1992-01-01

    The purpose of this paper is to study continuity of the parametrization of continuous-time linear time-invariant differential systems having a finite-dimensional state space. We show that convergence of the behavior of such systems corresponds to convergence of the coefficients of a set of associate

  13. Continuous Media Tasks Scheduling Algorithm

    Directory of Open Access Journals (Sweden)

    Myungryun Yoo

    2016-03-01

    Full Text Available In this paper the authors propose modified proportional share scheduling algorithm considering the characteristics of continuous media such as its continuity and time dependency. Proposed scheduling algorithm shows graceful degradation of performance in overloaded situation and decreases the number of context switching. Proposed scheduling algorithm is evaluated using several numerical tests under various conditions, especially overloaded situation.

  14. Continuous supersonic plasma wind tunnel

    DEFF Research Database (Denmark)

    Andersen, S.A.; Jensen, Vagn Orla; Nielsen, P.

    1968-01-01

    The B field configuration of a Q-device has been modified into a magnetic Laval nozzle. Continuous supersonic plasma flow is observed with M≈3......The B field configuration of a Q-device has been modified into a magnetic Laval nozzle. Continuous supersonic plasma flow is observed with M≈3...

  15. Continuing Education: Facing the Issues.

    Science.gov (United States)

    Broadbent, Marianne

    1986-01-01

    Examines a number of issues facing the Australian library and information services community in the area of continuing education, including recommendations of the Library Association of Australia, the cost of continuing education activities, the role and responsibility of schools of library and information studies, and notions of coordination.…

  16. Rational Exponentials and Continued Fractions

    Science.gov (United States)

    Denny, J. K.

    2012-01-01

    Using continued fraction expansions, we can approximate constants, such as pi and e, using an appropriate integer n raised to the power x[superscript 1/x], x a suitable rational. We review continued fractions and give an algorithm for producing these approximations.

  17. Completely Continuous and Weakly Completely Continuous Abstract Segal Algebras

    Indian Academy of Sciences (India)

    Fatemeh Abtahi

    2013-11-01

    Let $\\mathcal{A}$ be a Banach algebra. It is obtained a necessary and sufficient condition for the complete continuity and also weak complete continuity of symmetric abstract Segal algebras with respect to $\\mathcal{A}$, under the condition of the existence of an approximate identity for $\\mathcal{B}$, bounded in $\\mathcal{A}$. In addition, a necessary condition for the weak complete continuity of $\\mathcal{A}$ is given. Moreover, the applications of these results about some group algebras on locally compact groups are obtained.

  18. Continued professional competence and portfolios.

    Science.gov (United States)

    Byrne, Michelle; Delarose, Teresa; King, Cecil A; Leske, Jane; Sapnas, Kathryn G; Schroeter, Kathryn

    2007-01-01

    It is traditionally assumed that licensure of healthcare professionals means that they are minimally competent. Many nursing specialty organizations offer examinations and other processes for certification, suggesting that certification is associated with continued competency. Can standardized examination for certification and continuing education for recertification ensure continued competency? Continuing education and testing provide a limited picture of an individual's knowledge and/or skill acquisition in a limited area at one point in time. However, portfolios promote critical thinking, self-assessment, and individual accountability. A portfolio is a portable mechanism for evaluating competencies that may otherwise be difficult to assess. This article summarizes some of the literature addressing portfolios, including aspects of portfolio development process, the value of portfolios versus continuing education for competency assessment, evidence associated with portfolio usage, and suggestions for organizing nursing portfolios.

  19. Continuous cofinal maps on ultrafilters

    CERN Document Server

    Dobrinen, Natasha

    2011-01-01

    An ultrafilter $\\mathcal{U}$ on a countable base {\\em has continuous Tukey reductions} if whenever an ultrafilter $\\mathcal{V}$ is Tukey reducible to $\\mathcal{U}$, then every monotone cofinal map $f:\\mathcal{U}\\ra\\mathcal{V}$ is continuous when restricted to some cofinal subset of $\\mathcal{U}$. In the first part of the paper, we give mild conditions under which the property of having continuous Tukey reductions is inherited under Tukey reducibility. In particular, if $\\mathcal{U}$ is Tukey reducible to a p-point then $\\mathcal{U}$ has continuous Tukey reductions. In the second part, we show that any countable iteration of Fubini products of p-points has Tukey reductions which are continuous with respect to its topological Ramsey space of $\\vec{\\mathcal{U}}$-trees.

  20. Protein design using continuous rotamers.

    Directory of Open Access Journals (Sweden)

    Pablo Gainza

    2012-01-01

    Full Text Available Optimizing amino acid conformation and identity is a central problem in computational protein design. Protein design algorithms must allow realistic protein flexibility to occur during this optimization, or they may fail to find the best sequence with the lowest energy. Most design algorithms implement side-chain flexibility by allowing the side chains to move between a small set of discrete, low-energy states, which we call rigid rotamers. In this work we show that allowing continuous side-chain flexibility (which we call continuous rotamers greatly improves protein flexibility modeling. We present a large-scale study that compares the sequences and best energy conformations in 69 protein-core redesigns using a rigid-rotamer model versus a continuous-rotamer model. We show that in nearly all of our redesigns the sequence found by the continuous-rotamer model is different and has a lower energy than the one found by the rigid-rotamer model. Moreover, the sequences found by the continuous-rotamer model are more similar to the native sequences. We then show that the seemingly easy solution of sampling more rigid rotamers within the continuous region is not a practical alternative to a continuous-rotamer model: at computationally feasible resolutions, using more rigid rotamers was never better than a continuous-rotamer model and almost always resulted in higher energies. Finally, we present a new protein design algorithm based on the dead-end elimination (DEE algorithm, which we call iMinDEE, that makes the use of continuous rotamers feasible in larger systems. iMinDEE guarantees finding the optimal answer while pruning the search space with close to the same efficiency of DEE.Software is available under the Lesser GNU Public License v3. Contact the authors for source code.

  1. Resveratrol mediated cell death in cigarette smoke transformed breast epithelial cells is through induction of p21Waf1/Cip1 and inhibition of long patch base excision repair pathway

    Energy Technology Data Exchange (ETDEWEB)

    Mohapatra, Purusottam; Satapathy, Shakti Ranjan; Das, Dipon; Siddharth, Sumit [Cancer Biology Division, KIIT School of Biotechnology, KIIT University, Campus-11, Patia, Bhubaneswar, Orissa 751024 (India); Choudhuri, Tathagata [Institute of Life Sciences, Nalco Square, Bhubaneswar, Orissa 751023 (India); Department of Biotechnology, Visva Bharati University, Santiniketan, West Bengal (India); Kundu, Chanakya Nath, E-mail: cnkundu@gmail.com [Cancer Biology Division, KIIT School of Biotechnology, KIIT University, Campus-11, Patia, Bhubaneswar, Orissa 751024 (India)

    2014-03-15

    Cigarette smoking is a key factor for the development and progression of different cancers including mammary tumor in women. Resveratrol (Res) is a promising natural chemotherapeutic agent that regulates many cellular targets including p21, a cip/kip family of cyclin kinase inhibitors involved in DNA damage-induced cell cycle arrest and blocking of DNA replication and repair. We have recently shown that cigarette smoke condensate (CSC) prepared from commercially available Indian cigarette can cause neoplastic transformation of normal breast epithelial MCF-10A cell. Here we studied the mechanism of Res mediated apoptosis in CSC transformed (MCF-10A-Tr) cells in vitro and in vivo. Res mediated apoptosis in MCF-10A-Tr cells was a p21 dependent event. It increased the p21 protein expression in MCF-10A-Tr cells and MCF-10A-Tr cells-mediated tumors in xenograft mice. Res treatment reduced the tumor size(s) and expression of anti-apoptotic proteins (e.g. PI3K, AKT, NFκB) in solid tumor. The expressions of cell cycle regulatory (Cyclins, CDC-2, CDC-6, etc.), BER associated (Pol-β, Pol-δ, Pol-ε, Pol-η, RPA, Fen-1, DNA-Ligase-I, etc.) proteins and LP-BER activity decreased in MCF-10A-Tr cells but remain significantly unaltered in isogenic p21 null MCF-10A-Tr cells after Res treatment. Interestingly, no significant changes were noted in SP-BER activity in both the cell lines after Res exposure. Finally, it was observed that increased p21 blocks the LP-BER in MCF-10A-Tr cells by increasing its interaction with PCNA via competing with Fen-1 after Res treatment. Thus, Res caused apoptosis in CSC-induced cancer cells by reduction of LP-BER activity and this phenomenon largely depends on p21. - Highlights: • Resveratrol (Res) caused reduction of MCF-10A-Tr cell growth by inducing apoptosis. • Res caused cell cycle arrest and DNA damage in p21 dependent manner. • Res mediated LP-BER reduction in MCF-10A-Tr cells was a p21 dependent phenomenon. • Res inhibits BER and PI

  2. Intermittent redesign of continuous controllers

    Science.gov (United States)

    Gawthrop, Peter J.; Wang, Liuping

    2010-08-01

    The reverse-engineering idea developed by Maciejowski in the context of model-based predictive control is applied to the redesign of continuous-time compensators as intermittent controllers. Not only does this give a way of designing constrained input and state versions of continuous-time compensators but also provides a method for turning continuous-time compensators into event-driven versions. The procedure is illustrated by three examples: an event-driven PID controller relevant to the human balance control problem, a constrained version of the classical mechanical vibration absorber of den Hartog and an event driven and constrained vibration absorber.

  3. Continuous cofinal maps on ultrafilters

    OpenAIRE

    Dobrinen, Natasha

    2011-01-01

    An ultrafilter $\\mathcal{U}$ on a countable base {\\em has continuous Tukey reductions} if whenever an ultrafilter $\\mathcal{V}$ is Tukey reducible to $\\mathcal{U}$, then every monotone cofinal map $f:\\mathcal{U}\\ra\\mathcal{V}$ is continuous when restricted to some cofinal subset of $\\mathcal{U}$. In the first part of the paper, we give mild conditions under which the property of having continuous Tukey reductions is inherited under Tukey reducibility. In particular, if $\\mathcal{U}$ is Tukey ...

  4. Thermodynamics with Continuous Information Flow

    National Research Council Canada - National Science Library

    Horowitz, Jordan M; Esposito, Massimiliano

    2014-01-01

    .... We show that many important results previously derived for nonautonomous Maxwell demons can be recovered from our formalism and use a cycle decomposition to analyze the continuous information flow...

  5. Continuous Delivery and Quality Monitoring

    CERN Document Server

    CERN. Geneva

    2016-01-01

    After introducing Continuous Delivery, I will switch the topic and try to answer the question how much should we invest in quality and how to do it efficiently. My observations reveal that software quality is often considered as the slo...

  6. Competitive Strategy in Continuing Education.

    Science.gov (United States)

    Baden, Clifford

    1987-01-01

    Reviews strategic variables available to those planning continuing education marketing programs. Discusses generic competitive strategies: (1) overall cost leadership, (2) differentiation, and (3) specialization. Mentions several potential problems. (CH)

  7. Competitive Strategy in Continuing Education.

    Science.gov (United States)

    Baden, Clifford

    1987-01-01

    Reviews strategic variables available to those planning continuing education marketing programs. Discusses generic competitive strategies: (1) overall cost leadership, (2) differentiation, and (3) specialization. Mentions several potential problems. (CH)

  8. The Engineering Societies & Continuing Education.

    Science.gov (United States)

    Professional Engineer, 1979

    1979-01-01

    Gives a description of what the major engineering societies (ASCE, ASME, AICHE, and IEEE) are doing in the area of continuing education. The description includes the short courses, their costs, duration, type and scope of the content. (GA)

  9. The Engineering Societies & Continuing Education.

    Science.gov (United States)

    Professional Engineer, 1979

    1979-01-01

    Gives a description of what the major engineering societies (ASCE, ASME, AICHE, and IEEE) are doing in the area of continuing education. The description includes the short courses, their costs, duration, type and scope of the content. (GA)

  10. Asymmetric reactions in continuous flow

    Directory of Open Access Journals (Sweden)

    Xiao Yin Mak

    2009-04-01

    Full Text Available An overview of asymmetric synthesis in continuous flow and microreactors is presented in this review. Applications of homogeneous and heterogeneous asymmetric catalysis as well as biocatalysis in flow are discussed.

  11. Continuous smearing of Wilson Loops

    CERN Document Server

    Lohmayer, Robert

    2011-01-01

    Continuum smearing was introduced in section 4.1 of JHEP03, 064 (2006) as a meaningful continuum analogue of the well known set of lattice techniques by the same name. Here we apply continuous smearing in continuous space-time to Wilson loops in order to clarify what it does in the context of field theory and also in the context of the loop calculus of the Makeenko-Migdal equation.

  12. Continuous-Energy Data Checks

    Energy Technology Data Exchange (ETDEWEB)

    Haeck, Wim [Radioprotection and Nuclear Safety Institute, Fontenay-aux-Roses (France); Conlin, Jeremy Lloyd [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); McCartney, Austin Paul [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Parsons, Donald Kent [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2016-05-25

    The purpose of this report is to provide an overview of all Quality Assurance tests that have to be performed on a nuclear data set to be transformed into an ACE formatted nuclear data file. The ACE file is capable of containing different types of data such as continuous energy neutron data, thermal scattering data, etc. Within this report, we will limit ourselves to continuous energy neutron data.

  13. Capital Improvement Program (CIP) Project Status

    Data.gov (United States)

    Montgomery County of Maryland — This dataset includes pertinent information relating to a capital project’s status administered by the Department of Transportation and the Department of General...

  14. Polymer Science. Program CIP: 15.0607

    Science.gov (United States)

    Research and Curriculum Unit, 2010

    2010-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  15. The future of the ''intelligent Energy for Europe'' programme stakeholders consultation on the framework programme for competitiveness and innovation (CIP) Energie-Cites Opinion; L'Avenir du Programme ''Energie Intelligente Europe'' consultation des acteurs sur le programme pour la competitivite et l nnovation Avis d nergie-Cites

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-11-01

    The European Commission has launched a Consultation relative to the integration of support programmes to energy efficiency (EIE Programme) in the Competitiveness and Innovative Programme (CIP), this one being integrated in the Lisbon process. This consultation offers the opportunity to Energie-Cites to define its opinion on the relationships between Sustainable energy, Competitiveness and Innovation. - If Europe is to become the most competitive and innovative region in the world, it must consider this objective from the angle of sustainable growth, that is, by promoting the efficient use of fossil resources and by controlling the effluents and waste that are generated by the transformation of energy. What is at stake for Europe is its capacity to propose an energy model (consumption and production) that can be reproducible in the rest of the world. In other hands, the integration of energy efficiency and renewable energy support programmes into a broader scheme (CIP) covering very different areas, with no links between them and with no clear information regarding both the financial amounts and sources involved, entails a major risk, that of reducing, or even suppressing support at a time when we need it most. From the current understanding of the situation, Energie-Cites is not favourable to such integration. (A.L.B.)

  16. Copper recovery from gold contained sulphuric slag by acid leaching —Industrial production practices of gold recovery by CIP%含金硫酸烧渣酸浸铜—氰化炭浆法提金工艺生产实践

    Institute of Scientific and Technical Information of China (English)

    张静茹; 李晓文

    2012-01-01

    Bench scale tests on cyanidation - CIP process to leach gold and the industrial production practices indicate that it is technically viable to use CIP to deal with gold contained sulphuric slag. It not only achieves satisfactory technical indexes but also saves the infrastructural construction investment and shortens the construction term. The hematite content in the slag has less than considerable impact on the carbon adsorption.%陕西省某黄金冶炼厂含金硫酸烧渣酸浸铜—氰化炭浆法提金工艺的小型试验及工业生产实践的结果表明:采用炭浆法处理含金硫酸烧渣在技术上是可行的,不仅可以取得较好的技术指标,而且可以节省基建投资和缩短基建工期.含金硫酸烧渣中的赤铁矿对活性炭影响不大.

  17. Business continuity 2014: From traditional to integrated Business Continuity Management.

    Science.gov (United States)

    Ee, Henry

    As global change continues to generate new challenges and potential threats to businesses, traditional business continuity management (BCM) slowly reveals its limitations and weak points to ensuring 'business resiliency' today. Consequently, BCM professionals also face the challenge of re-evaluating traditional concepts and introducing new strategies and industry best practices. This paper points to why traditional BCM is no longer sufficient in terms of enabling businesses to survive in today's high-risk environment. It also looks into some of the misconceptions about BCM and other stumbling blocks to establishing effective BCM today. Most importantly, however, this paper provides tips based on the Business Continuity Institute's (BCI) Good Practices Guideline (GPG) and the latest international BCM standard ISO 22301 on how to overcome the issues and challenges presented.

  18. Continuous Approximations of a Class of Piecewise Continuous Systems

    Science.gov (United States)

    Danca, Marius-F.

    In this paper, we provide a rigorous mathematical foundation for continuous approximations of a class of systems with piecewise continuous functions. By using techniques from the theory of differential inclusions, the underlying piecewise functions can be locally or globally approximated. The approximation results can be used to model piecewise continuous-time dynamical systems of integer or fractional-order. In this way, by overcoming the lack of numerical methods for differential equations of fractional-order with discontinuous right-hand side, unattainable procedures for systems modeled by this kind of equations, such as chaos control, synchronization, anticontrol and many others, can be easily implemented. Several examples are presented and three comparative applications are studied.

  19. Coded continuous wave meteor radar

    Science.gov (United States)

    Vierinen, Juha; Chau, Jorge L.; Pfeffer, Nico; Clahsen, Matthias; Stober, Gunter

    2016-03-01

    The concept of a coded continuous wave specular meteor radar (SMR) is described. The radar uses a continuously transmitted pseudorandom phase-modulated waveform, which has several advantages compared to conventional pulsed SMRs. The coding avoids range and Doppler aliasing, which are in some cases problematic with pulsed radars. Continuous transmissions maximize pulse compression gain, allowing operation at lower peak power than a pulsed system. With continuous coding, the temporal and spectral resolution are not dependent on the transmit waveform and they can be fairly flexibly changed after performing a measurement. The low signal-to-noise ratio before pulse compression, combined with independent pseudorandom transmit waveforms, allows multiple geographically separated transmitters to be used in the same frequency band simultaneously without significantly interfering with each other. Because the same frequency band can be used by multiple transmitters, the same interferometric receiver antennas can be used to receive multiple transmitters at the same time. The principles of the signal processing are discussed, in addition to discussion of several practical ways to increase computation speed, and how to optimally detect meteor echoes. Measurements from a campaign performed with a coded continuous wave SMR are shown and compared with two standard pulsed SMR measurements. The type of meteor radar described in this paper would be suited for use in a large-scale multi-static network of meteor radar transmitters and receivers. Such a system would be useful for increasing the number of meteor detections to obtain improved meteor radar data products.

  20. A Dynamic Continuation-Passing Style for Dynamic Delimited Continuations

    DEFF Research Database (Denmark)

    Biernacki, Dariusz; Danvy, Olivier; Millikin, Kevin Scott

    2005-01-01

    -passing style.' We show that the new machine operates more efficiently than the definitional one and that the notion of computation induced by the corresponding evaluator takes the form of a monad. We also present new examples and a new simulation of dynamic delimited continuations in terms of static ones....

  1. Formal Verification of Continuous Systems

    DEFF Research Database (Denmark)

    Sloth, Christoffer

    2012-01-01

    and the verification procedures should be algorithmically synthesizable. Autonomous control plays an important role in many safety-critical systems. This implies that a malfunction in the control system can have catastrophic consequences, e.g., in space applications where a design flaw can result in large economic...... losses. Furthermore, a malfunction in the control system of a surgical robot may cause death of patients. The previous examples involve complex systems that are required to operate according to complex specifications. The systems cannot be formally verified by modern verification techniques, due......The purpose of this thesis is to develop a method for verifying timed temporal properties of continuous dynamical systems, and to develop a method for verifying the safety of an interconnection of continuous systems. The methods must be scalable in the number of continuous variables...

  2. Distributed synthesis in continuous time

    DEFF Research Database (Denmark)

    Hermanns, Holger; Krčál, Jan; Vester, Steen

    2016-01-01

    . Indeed, the explicit continuous time enables players to communicate their states by delaying synchronisation (which is unrestricted for non-urgent models). In general, the problems are undecidable already for two players in the quantitative case and three players in the qualitative case. The qualitative......We introduce a formalism modelling communication of distributed agents strictly in continuous-time. Within this framework, we study the problem of synthesising local strategies for individual agents such that a specified set of goal states is reached, or reached with at least a given probability....... The flow of time is modelled explicitly based on continuous-time randomness, with two natural implications: First, the non-determinism stemming from interleaving disappears. Second, when we restrict to a subclass of non-urgent models, the quantitative value problem for two players can be solved in EXPTIME...

  3. Discrete and Continuous Linearizable Equations

    CERN Document Server

    Lafortune, S; Ramani, A

    1998-01-01

    We study the projective systems in both continuous and discrete settings. These systems are linearizable by construction and thus, obviously, integrable. We show that in the continuous case it is possible to eliminate all variables but one and reduce the system to a single differential equation. This equation is of the form of those singled-out by Painlevé in his quest for integrable forms. In the discrete case, we extend previous results of ours showing that, again by elimination of variables, the general projective system can be written as a mapping for a single variable. We show that this mapping is a member of the family of multilinear systems (which is not integrable in general). The continuous limit of multilinear mappings is also discussed.

  4. Continuous biodisel productions: A review

    Directory of Open Access Journals (Sweden)

    Stamenković Ivica S.

    2009-01-01

    Full Text Available Continuous biodiesel production on laboratory and industrial scale was analyzed, with focus on their advantages and disadvantages. Attention was paid to specific characteristics of industrial processes in order to point out the advanced technologies. The well-known base-catalyzed continuous biodiesel production processes are related to problems caused by the immiscibility of the reactants (alcohol and oil, application of relatively high operating temperature (usually the boiling temperature of alcohol or one near it and obtained yield of methyl ester yields lower than desired. One way to overcome these problems is to employ special reactor design favoring the emulsion process and increasing the overall rate of biodiesel production process, even at room temperature and atmospheric pressure. The second way is to apply heterogeneous catalysts in continuous processes, which will probably be the optimal approach to economically justified and environmentally friendly biodiesel production.

  5. Continuous lengths of oxide superconductors

    Science.gov (United States)

    Kroeger, Donald M.; List, III, Frederick A.

    2000-01-01

    A layered oxide superconductor prepared by depositing a superconductor precursor powder on a continuous length of a first substrate ribbon. A continuous length of a second substrate ribbon is overlaid on the first substrate ribbon. Sufficient pressure is applied to form a bound layered superconductor precursor powder between the first substrate ribbon and the second substrate ribbon. The layered superconductor precursor is then heat treated to establish the oxide superconducting phase. The layered oxide superconductor has a smooth interface between the substrate and the oxide superconductor.

  6. Approximation of continuously differentiable functions

    CERN Document Server

    Llavona, JG

    1986-01-01

    This self-contained book brings together the important results of a rapidly growing area.As a starting point it presents the classic results of the theory. The book covers such results as: the extension of Wells'' theorem and Aron''s theorem for the fine topology of order m; extension of Bernstein''s and Weierstrass'' theorems for infinite dimensional Banach spaces; extension of Nachbin''s and Whitney''s theorem for infinite dimensional Banach spaces; automatic continuity of homomorphisms in algebras of continuously differentiable functions, etc.

  7. LANL continuity of operations plan

    Energy Technology Data Exchange (ETDEWEB)

    Senutovitch, Diane M [Los Alamos National Laboratory

    2010-12-22

    The Los Alamos National Laboratory (LANL) is a premier national security research institution, delivering scientific and engineering solutions for the nation's most crucial and complex problems. Our primary responsibility is to ensure the safety, security, and reliability of the nation's nuclear stockpile. LANL emphasizes worker safety, effective operational safeguards and security, and environmental stewardship, outstanding science remains the foundation of work at the Laboratory. In addition to supporting the Laboratory's core national security mission, our work advances bioscience, chemistry, computer science, earth and environmental sciences, materials science, and physics disciplines. To accomplish LANL's mission, we must ensure that the Laboratory EFs continue to be performed during a continuity event, including localized acts of nature, accidents, technological or attack-related emergencies, and pandemic or epidemic events. The LANL Continuity of Operations (COOP) Plan documents the overall LANL COOP Program and provides the operational framework to implement continuity policies, requirements, and responsibilities at LANL, as required by DOE 0 150.1, Continuity Programs, May 2008. LANL must maintain its ability to perform the nation's PMEFs, which are: (1) maintain the safety and security of nuclear materials in the DOE Complex at fixed sites and in transit; (2) respond to a nuclear incident, both domestically and internationally, caused by terrorist activity, natural disaster, or accident, including mobilizing the resources to support these efforts; and (3) support the nation's energy infrastructure. This plan supports Continuity of Operations for Los Alamos National Laboratory (LANL). This plan issues LANL policy as directed by the DOE 0 150.1, Continuity Programs, and provides direction for the orderly continuation of LANL EFs for 30 days of closure or 60 days for a pandemic/epidemic event. Initiation of COOP operations may

  8. Continuous Lunches Are Not Free

    OpenAIRE

    Vose, Michael D.

    2015-01-01

    Stemming from a paper of Auger and Teytaud, there is a common misconception that for continuous domains No Free Lunch (NFL) does not hold. However, Rowe, Vose, and Wright have demonstrated that NFL holds for arbitrary domains and co-domains. This paper resolves the apparent contradiction.

  9. Marketing Continuing Education for Nurses.

    Science.gov (United States)

    Southern Regional Education Board, Atlanta, GA.

    This guide presents an overview of marketing and its potential value in continuing education programs for nurses. The first portion of the guide briefly discusses the concept of marketing. It contains definitions of key marketing concepts (product, place, price, and promotion), discussion of the basic tenets of marketing (consumer needs…

  10. China Continues Its FY Program

    Institute of Scientific and Technical Information of China (English)

    SunQing

    2004-01-01

    China will continue its FY program by launching FY-2C atop LM-3A launch vehicle probably in October 2004. The geostationary meteorological satellite FY2C will be deployed to replace FY-2B launched on June 25, 2000.

  11. Workload Control with Continuous Release

    NARCIS (Netherlands)

    Phan, B. S. Nguyen; Land, M. J.; Gaalman, G. J. C.

    2009-01-01

    Workload Control (WLC) is a production planning and control concept which is suitable for the needs of make-to-order job shops. Release decisions based on the workload norms form the core of the concept. This paper develops continuous time WLC release variants and investigates their due date

  12. Continuous supersonic plasma wind tunnel

    DEFF Research Database (Denmark)

    Andersen, S.A.; Jensen, Vagn Orla; Nielsen, P.

    1969-01-01

    The normal magnetic field configuration of a Q device has been modified to obtain a 'magnetic Laval nozzle'. Continuous supersonic plasma 'winds' are obtained with Mach numbers ~3. The magnetic nozzle appears well suited for the study of the interaction of supersonic plasma 'winds' with either...

  13. Nasal continuous positive airway pressure

    DEFF Research Database (Denmark)

    Scholze, Alexandra; Lamwers, Stephanie; Tepel, Martin

    2012-01-01

    Obstructive sleep apnoea (OSA) is linked to increased cardiovascular risk. This risk can be reduced by nasal continuous positive airway pressure (nCPAP) treatment. As OSA is associated with an increase of several vasoconstrictive factors, we investigated whether nCPAP influences the digital volume...

  14. Continuous Non-malleable Codes

    DEFF Research Database (Denmark)

    Faust, Sebastian; Mukherjee, Pratyay; Nielsen, Jesper Buus

    2014-01-01

    is based on the inner product function, collision-resistant hashing and non-interactive zero-knowledge proofs of knowledge and requires an untamperable common reference string. We apply continuous non-malleable codes to protect arbitrary cryptographic primitives against tampering attacks. Previous...

  15. Midwives' Motivation for Continuing Education.

    Science.gov (United States)

    Laszlo, Halldora; Strettle, Robert J.

    1996-01-01

    Responses from 83 of 120 British midwives showed that motivation for continuing education was strongest in regard to professional competence and innate desire for knowledge, less in regard to legal requirements for practice. Strongest motivators were internal and learning related. Social integration was the least important aspect of continuing…

  16. Unconditional Continuous Variable Dense Coding

    CERN Document Server

    Ralph, T C

    2002-01-01

    We investigate the conditions under which unconditional dense coding can be achieved using continuous variable entanglement. We consider the effect of entanglement impurity and detector efficiency and discuss experimental verification. We conclude that the requirements for a strong demonstration are not as stringent as previously thought and are within the reach of present technology.

  17. Connecting the Dots: Rediscovering Continuity

    Science.gov (United States)

    Camenga, Kristin A.; Yates, Rebekah B. Johnson

    2014-01-01

    The topic of continuity is typically not introduced until calculus and then reexamined in real analysis. Recognizing the connections between secondary school mathematics and the advanced mathematics studied at the college level allows teachers to better identify mathematical concepts in student ideas, motivate students by piquing their curiosity,…

  18. For Time-Continuous Optimisation

    DEFF Research Database (Denmark)

    Heinrich, Mary Katherine; Ayres, Phil

    2016-01-01

    Strategies for optimisation in design normatively assume an artefact end-point, disallowing continuous architecture that engages living systems, dynamic behaviour, and complex systems. In our Flora Robotica investigations of symbiotic plant-robot bio-hybrids, we re- quire computational tools...

  19. In the interest of continuity

    CERN Multimedia

    Association du personnel

    2009-01-01

    Every five years a new Management takes over the fifth floor of the Main Building. The arrival of a new Director-General comes with a desire to change the managerial style. Whilst not contesting the current need for this, we would like to remind you that continuity in the Organization is ensured by the personnel and its Staff Association representatives.

  20. For Time-Continuous Optimisation

    DEFF Research Database (Denmark)

    Heinrich, Mary Katherine; Ayres, Phil

    2016-01-01

    Strategies for optimisation in design normatively assume an artefact end-point, disallowing continuous architecture that engages living systems, dynamic behaviour, and complex systems. In our Flora Robotica investigations of symbiotic plant-robot bio-hybrids, we re- quire computational tools...

  1. Connecting the Dots: Rediscovering Continuity

    Science.gov (United States)

    Camenga, Kristin A.; Yates, Rebekah B. Johnson

    2014-01-01

    The topic of continuity is typically not introduced until calculus and then reexamined in real analysis. Recognizing the connections between secondary school mathematics and the advanced mathematics studied at the college level allows teachers to better identify mathematical concepts in student ideas, motivate students by piquing their curiosity,…

  2. Pythagorean Approximations and Continued Fractions

    Science.gov (United States)

    Peralta, Javier

    2008-01-01

    In this article, we will show that the Pythagorean approximations of [the square root of] 2 coincide with those achieved in the 16th century by means of continued fractions. Assuming this fact and the known relation that connects the Fibonacci sequence with the golden section, we shall establish a procedure to obtain sequences of rational numbers…

  3. Coded continuous wave meteor radar

    Directory of Open Access Journals (Sweden)

    J. Vierinen

    2015-07-01

    Full Text Available The concept of coded continuous wave meteor radar is introduced. The radar uses a continuously transmitted pseudo-random waveform, which has several advantages: coding avoids range aliased echoes, which are often seen with commonly used pulsed specular meteor radars (SMRs; continuous transmissions maximize pulse compression gain, allowing operation with significantly lower peak transmit power; the temporal resolution can be changed after performing a measurement, as it does not depend on pulse spacing; and the low signal to noise ratio allows multiple geographically separated transmitters to be used in the same frequency band without significantly interfering with each other. The latter allows the same receiver antennas to be used to receive multiple transmitters. The principles of the signal processing are discussed, in addition to discussion of several practical ways to increase computation speed, and how to optimally detect meteor echoes. Measurements from a campaign performed with a coded continuous wave SMR are shown and compared with two standard pulsed SMR measurements. The type of meteor radar described in this paper would be suited for use in a large scale multi-static network of meteor radar transmitters and receivers. This would, for example, provide higher spatio-temporal resolution for mesospheric wind field measurements.

  4. Coded continuous wave meteor radar

    Science.gov (United States)

    Vierinen, J.; Chau, J. L.; Pfeffer, N.; Clahsen, M.; Stober, G.

    2015-07-01

    The concept of coded continuous wave meteor radar is introduced. The radar uses a continuously transmitted pseudo-random waveform, which has several advantages: coding avoids range aliased echoes, which are often seen with commonly used pulsed specular meteor radars (SMRs); continuous transmissions maximize pulse compression gain, allowing operation with significantly lower peak transmit power; the temporal resolution can be changed after performing a measurement, as it does not depend on pulse spacing; and the low signal to noise ratio allows multiple geographically separated transmitters to be used in the same frequency band without significantly interfering with each other. The latter allows the same receiver antennas to be used to receive multiple transmitters. The principles of the signal processing are discussed, in addition to discussion of several practical ways to increase computation speed, and how to optimally detect meteor echoes. Measurements from a campaign performed with a coded continuous wave SMR are shown and compared with two standard pulsed SMR measurements. The type of meteor radar described in this paper would be suited for use in a large scale multi-static network of meteor radar transmitters and receivers. This would, for example, provide higher spatio-temporal resolution for mesospheric wind field measurements.

  5. Coded continuous wave meteor radar

    Science.gov (United States)

    Chau, J. L.; Vierinen, J.; Pfeffer, N.; Clahsen, M.; Stober, G.

    2016-12-01

    The concept of a coded continuous wave specular meteor radar (SMR) is described. The radar uses a continuously transmitted pseudorandom phase-modulated waveform, which has several advantages compared to conventional pulsed SMRs. The coding avoids range and Doppler aliasing, which are in some cases problematic with pulsed radars. Continuous transmissions maximize pulse compression gain, allowing operation at lower peak power than a pulsed system. With continuous coding, the temporal and spectral resolution are not dependent on the transmit waveform and they can be fairly flexibly changed after performing a measurement. The low signal-to-noise ratio before pulse compression, combined with independent pseudorandom transmit waveforms, allows multiple geographically separated transmitters to be used in the same frequency band simultaneously without significantly interfering with each other. Because the same frequency band can be used by multiple transmitters, the same interferometric receiver antennas can be used to receive multiple transmitters at the same time. The principles of the signal processing are discussed, in addition to discussion of several practical ways to increase computation speed, and how to optimally detect meteor echoes. Measurements from a campaign performed with a coded continuous wave SMR are shown and compared with two standard pulsed SMR measurements. The type of meteor radar described in this paper would be suited for use in a large-scale multi-static network of meteor radar transmitters and receivers. Such a system would be useful for increasing the number of meteor detections to obtain improved meteor radar data products, such as wind fields. This type of a radar would also be useful for over-the-horizon radar, ionosondes, and observations of field-aligned-irregularities.

  6. Continuous sampling of MSWI dioxins.

    Science.gov (United States)

    Hung, Pao-Chen; Chang, Shu-Hao; Buekens, Alfons; Chang, Moo-Been

    2016-02-01

    Municipal solid waste incineration (MSWI) is generally considered as a well-controlled source of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), in brief dioxins. Start-up conditions continue, however, to be problematic. A self-developed continuous sampler was specially designed and built to fulfill the various sampling criteria of U.S. EPA Method 23 and monitor the trends of dioxins emissions during diverse operating conditions. In the MSWI plant investigated, a 98.1% TEQ PCDD/F removal efficiency was achieved in normal operation using activated carbon injection + bag filtration (ACI + BF) and the corresponding PCDD/F emission remains well below the standard set by Taiwan EPA (0.1 ng I-TEQ/Nm(3) @ 11% O2). During start-up, however, continuous sampling indicates that this limit value is reached only after 12 and 9 days, respectively for the 1st (2011) and 2nd test campaign (2012). Only 15 days after start-up the PCDD/F emissions shrunk to the levels typically measured during normal operation. The PCDD/F emissions from the 1st and 2nd continuous sampling campaigns were 5.4 and 5.5 mg I-TEQ, respectively. Short-term PCDD/F sampling such as the U.S. EPA Method 23 is less adapted to monitor these transient PCDD/F emissions representatively and accurately, due to a steady decrease of PCDD/F emissions after start-up. This self-developed continuous sampler effectively enhances the ease and reliability of emission data collecting during transient conditions of MSWI.

  7. Importance of Knowledge Continuity in Business Continuity Management

    OpenAIRE

    Hana Urbancová; Kateřina Venclová

    2013-01-01

    At present, the real generator of wealth in society, as well as in the economy as a whole, is knowledge. Knowledge that is critical and precious for an organization (whose loss would represent a threat for the organisation) and that is valuable for competitors needs to be treated with care. Knowledge continuity management is, also, applied to preserve critical knowledge in an organisation in situations where its holder leaves to join a competitor or to retire. The article aims to identify the...

  8. Recomposition des jeux d’acteurs et concurrence pour l’espace face à la mise en place d’un Centre touristique Intégralement  Planifié (CIP

    Directory of Open Access Journals (Sweden)

    Clément Marie dit Chirot

    2011-09-01

    Full Text Available En développant, à partir de 1984, un Centre touristique Intégralement Planifié dans une localité de la côte Pacifique de l’état d’Oaxaca, l’Etat mexicain entendait impulser un développement économique dans une région périphérique et pauvre du pays. Basé sur l’expropriation d’une portion du territoire communal, le processus engendre un certain nombre de résistances au sein des communautés locales. Le centre touristique est aujourd’hui un espace fragmenté, reflet et conséquence des inégalités sociales et des conflits sociaux. Depuis une perspective de géographie sociale, cet article cherche à mettre en lumière les conflits et compromis sociaux qui traversent la société locale. L’appropriation de l’espace, les luttes de pouvoir et les stratégies développées par les différents acteurs seront les entrées privilégiées pour interroger un modèle de planification, « par le haut », ignorant trop souvent les réalités sociales à l’échelle locale.Since 1984, the Mexican government has tried to stimulate the economy in a poor and peripheral area of the country. They aimed to do so by developing an Integrally Planed Resort (CIP, in Spanish in a community, located in the Oaxaca state on the Pacific coast. Being based on the expropriation of some parts of the communal territory, this process created some remittances among the local communities. Today, this resort is still a fragmented space and reflects social inequalities and social conflicts. From a social geographical perspective, this article aims to show the social conflicts and social compromises among the local society. Space appropriation, power battles and strategies developed by the different actors, will be the main tools to investigate this planning model. In this model, the government too often has ignored social realities on the local scale.Al crear, a partir de 1984, un Centro turístico Integralmente Planeado en un poblado de la costa del

  9. A Tubular Electrochemical Reactor for Continuous Generation of Sodium Hypochlorite for Anti-Microbial Applications%一种连续产生次氯酸钠的管状电化学反应杀菌器

    Institute of Scientific and Technical Information of China (English)

    曾新安; 秦贯丰; 李忠彦

    2005-01-01

    介绍了一种中试规模的用于原位清洗(CIP)或原位灭菌(SIP)的次氯酸钠连续发生器.其电解槽采用带尼龙隔膜的管式电化学反应器结构,并在钛阳极管的表面采用稀土金属氧化物二氧化钌(RuO2)作为电化学反应的活性催化涂层.文中从反应工程的角度分析了该发生器的性能.试验结果表明,该反应器的电流效率在82%以上.微生物学研究表明,有效氯含量范围内的消毒水可有效杀灭大部分食品工业设备表面所发现的微生物.%A pilot-scale reactor for continuously generating sodium hypochlorite (NaClO), which is applied to the cleaning-in-place (CIP) and the sterilizing-in-place (SIP), is introduced in this paper. The electrolytic cell of the reactor is constructed as a tubular electrochemical reactor with a nylon net membrane between the electrodes and with a layer of rare-earth metallic oxide, RuO2, as the active catalytic coating on the titanium anode surface. The performance of this generator is then analyzed from the point of view of reaction engineering.Experimental results indicate that the electric current efficiency of the reactor is above 82%. Biological study shows that the sterilizing water with the available chlorine content can effectively kill most microorganisms on the walls of food equipment.

  10. Black holes by analytic continuation

    CERN Document Server

    Amati, Daniele

    1997-01-01

    In the context of a two-dimensional exactly solvable model, the dynamics of quantum black holes is obtained by analytically continuing the description of the regime where no black hole is formed. The resulting spectrum of outgoing radiation departs from the one predicted by the Hawking model in the region where the outgoing modes arise from the horizon with Planck-order frequencies. This occurs early in the evaporation process, and the resulting physical picture is unconventional. The theory predicts that black holes will only radiate out an energy of Planck mass order, stabilizing after a transitory period. The continuation from a regime without black hole formation --accessible in the 1+1 gravity theory considered-- is implicit in an S matrix approach and provides in this way a possible solution to the problem of information loss.

  11. Personal continuous route pattern mining

    Institute of Scientific and Technical Information of China (English)

    Qian YE; Ling CHEN; Gen-cai CHEN

    2009-01-01

    In the daily life, people often repeat regular routes in certain periods. In this paper, a mining system is developed to find the continuous route patterns of personal past trips. In order to count the diversity of personal moving status, the mining system employs the adaptive GPS data recording and five data filters to guarantee the clean trips data. The mining system uses a client/server architecture to protect personal privacy and to reduce the computational load. The server conducts the main mining procedure but with insufficient information to recover real personal routes. In order to improve the scalability of sequential pattern mining, a novel pattern mining algorithm, continuous route pattern mining (CRPM), is proposed. This algorithm can tolerate the different disturbances in real routes and extract the frequent patterns. Experimental results based on nine persons' trips show that CRPM can extract more than two times longer route patterns than the traditional route pattern mining algorithms.

  12. Continuous-variable quantum games

    Energy Technology Data Exchange (ETDEWEB)

    Li Hui; Du Jiangfeng; Massar, Serge

    2002-12-30

    We investigate the quantization of games in which the players can access to a continuous set of classical strategies, making use of continuous-variable quantum systems. For the particular case of the Cournot's duopoly, we find that, even though the two players both act as 'selfishly' in the quantum game as they do in the classical game, they are found to virtually cooperate due to the quantum entanglement between them. We also find that the original Einstein-Podolksy-Rosen state contributes to the best profits that the two firms could ever attain. Moreover, we propose a practical experimental setup for the implementation of such quantum games.

  13. PIPER Continuous Adiabatic Demagnetization Refrigerator

    Science.gov (United States)

    Kimball, Mark O.; Shirron, Peter J.; Canavan, Edgar R.; James, Bryan L.; Sampson, Michael A.; Letmate, Richard V.

    2017-01-01

    We report upon the development and testing of a 4-stage adiabatic demagnetization refrigerator (ADR) capable of continuous cooling at 0.100 Kelvin. This cooler is being built to cool the detector array aboard NASA's Primordial Inflation Polarization Explorer (PIPER) observatory. The goal of this balloon mission is to measure the primordial gravitational waves that should exist if the theory of cosmological inflation is correct. At altitude, the ADR will hold the array of transition-edge sensors at 100 mK continuously while periodically rejecting heat to a 1.2 K pumped helium bath. During testing on ground, the array is held at the same temperature but heat is rejected to a 4.2 K helium bath indicating the flexibility in this coolers design.

  14. Monolithic Continuous-Flow Bioreactors

    Science.gov (United States)

    Stephanopoulos, Gregory; Kornfield, Julia A.; Voecks, Gerald A.

    1993-01-01

    Monolithic ceramic matrices containing many small flow passages useful as continuous-flow bioreactors. Ceramic matrix containing passages made by extruding and firing suitable ceramic. Pores in matrix provide attachment medium for film of cells and allow free movement of solution. Material one not toxic to micro-organisms grown in reactor. In reactor, liquid nutrients flow over, and liquid reaction products flow from, cell culture immobilized in one set of channels while oxygen flows to, and gaseous reaction products flow from, culture in adjacent set of passages. Cells live on inner surfaces containing flowing nutrient and in pores of walls of passages. Ready access to nutrients and oxygen in channels. They generate continuous high yield characteristic of immobilized cells, without large expenditure of energy otherwise incurred if necessary to pump nutrient solution through dense biomass as in bioreactors of other types.

  15. Quantum teleportation with continuous measurements

    Science.gov (United States)

    Greplova, Eliska; Mølmer, Klaus; Andersen, Christian Kraglund

    2016-10-01

    We propose a scheme for quantum teleportation between two qubits, coupled sequentially to a cavity field. An implementation of the scheme is analyzed with superconducting qubits and a transmission line resonator, where measurements are restricted to continuous probing of the field leaking from the resonator rather than instantaneous projective Bell state measurement. We show that the past quantum state formalism S. Gammelmark, B. Julsgaard, and K. Mølmer, Phys. Rev. Lett. 111, 160401 (2013), 10.1103/PhysRevLett.111.160401 can be successfully applied to estimate what would have been the most likely Bell measurement outcome conditioned on our continuous signal record. This information determines which local operation on the target qubit yields the optimal teleportation fidelity. Our results emphasize the significance of applying a detailed analysis of quantum measurements in feedforward protocols in nonideal leaky quantum systems.

  16. BRST theory for continuous spin

    Science.gov (United States)

    Bengtsson, Anders K. H.

    2013-10-01

    Some puzzling aspects of higher spin field theory in Minkowski space-time, such as the tracelessness constraints and the search for an underlying physical principle, are discussed. A connecting idea might be provided by the recently much researched continuous spin representations of the Poincaré group. The Wigner equations, treated as first class constraints, yields to a four-constraint BRST formulation. The resulting field theory, generalizing free higher spin field theory, is one among a set of higher spin theories that can be related to previous work on unconstrained formulations. In particular, it is conjectured that the unconstrained higher spin theory of Francia and Sagnotti is a limit of a continuous spin theory. Furthermore, a simple analysis of the constraint structure reveals a hint of a physical rationale behind the trace constraints.

  17. BRST Theory for Continuous Spin

    CERN Document Server

    Bengtsson, Anders K H

    2013-01-01

    Some puzzling aspects of higher spin field theory in Minkowski space-time, such as the tracelessness constraints and the search for an underlying physical principle, are discussed. A connecting idea might be provided by the recently much researched continuous spin representations of the Poincar\\'e group. The Wigner equations, treated as first class constraints, yields to a four-constraint BRST formulation. The resulting field theory, generalizing free higher spin field theory, is one among a set of higher spin theories that can be related to previous work on unconstrained formulations. In particular, it is conjectured that the unconstrained higher spin theory of Francia and Sagnotti is a limit of a continuous spin theory. Furthermore, a simple analysis of the constraint structure reveals a hint of a physical rationale behind the trace constraints.

  18. Continuous-Variable Entanglement Swapping

    Directory of Open Access Journals (Sweden)

    Kevin Marshall

    2015-05-01

    Full Text Available We present a very brief overview of entanglement swapping as it relates to continuous-variable quantum information. The technical background required is discussed and the natural link to quantum teleportation is established before discussing the nature of Gaussian entanglement swapping. The limitations of Gaussian swapping are introduced, along with the general applications of swapping in the context of to quantum communication and entanglement distribution. In light of this, we briefly summarize a collection of entanglement swapping schemes which incorporate a non-Gaussian ingredient and the benefits of such schemes are noted. Finally, we motivate the need to further study and develop such schemes by highlighting requirements of a continuous-variable repeater.

  19. Dental Continuing Education Preference Survey

    Science.gov (United States)

    1992-06-01

    the 63A9Ds and 34.9% for the 63A00s, selected Comprehensive Dentistry as their specialty preference. For the 63A9Ds, orthodontics , periodontics, and...prosthodontics were the next most frequently selected specialties. For the 63A00s, it was orthodontics , prosthodontics, and endodontics. Nearly 10% of...education. International Dental Journal , 28(2): 149-153, 1978. 7. Haroth, R.W. & Halpern, D.F. Maryland Continuing Dental Education Survey: "Your

  20. Analytic continuation of Toeplitz operators

    OpenAIRE

    Bommier-Hato, H.; Engliš, M. (Miroslav); Youssfi, E.-H.

    2015-01-01

    Generalizing results of Rossi and Vergne for the holomorphic discrete series on symmetric domains, on the one hand, and of Chailuek and Hall for Toeplitz operators on the ball, on the other hand, we establish existence of analytic continuation of weighted Bergman spaces, in the weight (Wallach) parameter, as well as of the associated Toeplitz operators (with sufficiently nice symbols), on any smoothly bounded strictly pseudoconvex domain. Still further extension to Sobolev spaces of holomorph...

  1. Efeitos da luz e da temperatura na germinação de sementes de Eremanthus (Asteraceae, ocorrentes na Serra do Cipó, MG, Brasil Light and temperature effects in the germination of seeds of Eremanthus (Asteraceae that occur in Serra do Cipó, Minas Gerais State, Brazil

    Directory of Open Access Journals (Sweden)

    Simone Batalha Velten

    2005-12-01

    Full Text Available Estudos de germinação são importantes para a propagação de plantas, regeneração e conservação de comunidades vegetais. O objetivo deste trabalho foi avaliar a viabilidade e o comportamento germinativo de sementes de Eremanthus elaeagnus (Mart. ex DC. Schultz-Bip, E. glomerulatus Less e E. Incanus (Less. Less. A viabilidade das sementes foi verificada através do teste de tetrazólio. Os testes de germinação foram realizados sob luz e escuro contínuos em temperaturas constantes de 15, 20, 25, 30, 35 e 40 ºC e em temperaturas alternadas (fotoperíodo de 12 h de 15-25, 15-30, 15-35, 20-30, 20-35 e 25-35 ºC. A viabilidade das sementes variou significativamente entre populações e diferentes anos de coleta. As percentagens de germinação das sementes foram sempre baixas, devido, principalmente, à ausência de embriões. Sementes de E. elaeagnus germinaram nas temperaturas de 15 a 30ºC, de E. glomerulatus de 20 a 30 ºC e E. incanus de 15 a 35 ºC. As três espécies germinaram na luz e no escuro, apresentando diferenças significativas entre estes dois regimes apenas nas temperaturas de 25 e 35 ºC (E. incanus e 30 ºC (E. glomerulatus. Em comparação com as outras espécies Eremanthus incanus apresentou maiores percentagens de germinação em faixa de temperatura mais ampla e suas sementes são mais rápidas para germinar.Studies of germination are important for the propagation of plants, and the regeneration and the preservation of plant communities. The purpose of this study was to evaluate the viability and the germinative behavior of the seeds of Eremanthus elaeagnus (Mart. ex DC. Schultz-Bip , E. glomerulatus Less. and E. incamus (Less Less. The viability of seeds was confirmed through the tetrazolium test. Germination tests were conducted under continuous light and darkness at constant temperatures of 15, 20, 25, 30, 35 and 40 ºC and at alternated temperatures (photoperiod of 12 hours range from 15-25, 15-30, 15-35, 20

  2. Topological Photonics for Continuous Media

    Science.gov (United States)

    Silveirinha, Mario

    Photonic crystals have revolutionized light-based technologies during the last three decades. Notably, it was recently discovered that the light propagation in photonic crystals may depend on some topological characteristics determined by the manner how the light states are mutually entangled. The usual topological classification of photonic crystals explores the fact that these structures are periodic. The periodicity is essential to ensure that the underlying wave vector space is a closed surface with no boundary. In this talk, we prove that it is possible calculate Chern invariants for a wide class of continuous bianisotropic electromagnetic media with no intrinsic periodicity. The nontrivial topology of the relevant continuous materials is linked with the emergence of edge states. Moreover, we will demonstrate that continuous photonic media with the time-reversal symmetry can be topologically characterized by a Z2 integer. This novel classification extends for the first time the theory of electronic topological insulators to a wide range of photonic platforms, and is expected to have an impact in the design of novel photonic systems that enable a topologically protected transport of optical energy. This work is supported in part by Fundacao para a Ciencia e a Tecnologia Grant Number PTDC/EEI-TEL/4543/2014.

  3. From turnaround to continuous innovation

    DEFF Research Database (Denmark)

    Bjarnø, Ole-Christian

    2002-01-01

    sourcing, explorative case research and continuous innovation a series of findings is identified, and from these findings a management model is proposed. This model is intended to fill in an identified gap in the toolbox for maintenance and further development of the business partners network established......In this article a five year longitudinal study performed at a medium-sized producer of electronics is reported during which we studied the company's change process. The paper deals with the concept of strategic sourcing as a component in turnaround change management. Based on theories of strategic...

  4. From turnaround to continuous innovation

    DEFF Research Database (Denmark)

    Bjarnø, Ole-Christian

    2002-01-01

    sourcing, explorative case research and continuous innovation a series of findings is identified, and from these findings a management model is proposed. This model is intended to fill in an identified gap in the toolbox for maintenance and further development of the business partners network established......In this article a five year longitudinal study performed at a medium-sized producer of electronics is reported during which we studied the company's change process. The paper deals with the concept of strategic sourcing as a component in turnaround change management. Based on theories of strategic...

  5. Now f is continuous (exercise!

    Directory of Open Access Journals (Sweden)

    Robin Denis Arthan

    2016-01-01

    Full Text Available A recurring proof obligation in modern mathematics, ranging from textbook exercises to deep research problems, is to show that a given function is a morphism in some category: in analysis and topology, for example, we frequently need to prove that functions are continuous, while in group theory we are constantly concerned with homomorphisms. This paper describes a generic procedure that automatically discharges routine instances of this kind of proof obligation in an interactive theorem prover. The proof procedure has been implemented and found very useful in a mathematical case studies carried out using the ProofPower system

  6. FACTORS INFLUENCING CONTINUOUS ORGANISATIONAL CHANGE

    Directory of Open Access Journals (Sweden)

    Alexandru Rizescu

    2016-10-01

    Full Text Available Change involves the continuous adjustment to the external conditions of organizations in the operating environment, in parallel with the growth of domestic stability. This process constitutes the dilemma of change-stability, which can be tackled only through a vision of the future, meaning the idorganization of organization-environment interaction along with a flexible organizational structure, the use of advanced technology and the existence of a system of rewarding employees that reflects the values and priorities of both, organizational norms and individual needs.

  7. Continuous-state branching processes

    CERN Document Server

    Li, Zenghu

    2012-01-01

    These notes were used in a short graduate course on branching processes the author gave in Beijing Normal University. The following main topics are covered: scaling limits of Galton--Watson processes, continuous-state branching processes, extinction probabilities, conditional limit theorems, decompositions of sample paths, martingale problems, stochastic equations, Lamperti's transformations, independent and dependent immigration processes. Some of the results are simplified versions of those in the author's book "Measure-valued branching Markov processes" (Springer, 2011). We hope these simplified results will set out the main ideas in an easy way and lead the reader to a quick access of the subject.

  8. Continuing education for hospice staff.

    Science.gov (United States)

    Conedera, F; Schoessler, M

    1985-06-01

    Hospice nursing is unique because of the philosophy and issues surrounding hospice care. Program planning for hospice staff follows basic principles. The real challenge in developing programs for orientation, continuing, and inservice education is using a format that will truly enable staff to meet the objectives. A lecture, programmed instruction, or video/slide format works well for the "nuts and bolts," but more creativity is needed for the other issues facing the hospice nurse--death, grief, symptom control, stress, team roles, and helping patients with options. Incorporating into the program some of the methods suggested will offer staff the opportunity to become involved in learning and make that learning more meaningful.

  9. Continuously-Variable Vernier Scale

    Science.gov (United States)

    Miller, Irvin M.

    1989-01-01

    Easily fabricated device increases precision in reading graphical data. Continuously-variable vernier scale (CV VS) designed to provide greater accuracy to scientists and technologists in reading numerical values from graphical data. Placed on graph and used to interpolate coordinate value of point on curve or plotted point on figure within division on each coordinate axis. Requires neither measurement of line segments where projection of point intersects division nor calculation to quantify projected value. Very flexible device constructed with any kind of scale. Very easy to use, requiring no special equipment of any kind, and saves considerable amount of time if numerous points to be evaluated.

  10. South Africa: defiance campaign continues.

    Science.gov (United States)

    2002-03-01

    The Treatment Action Campaign (TAC) has continued its "defiance campaign against patent abuse and AIDS profiteering." In partnership with Médecins Sans Frontières (Doctors Without Borders), and with the support of Oxfam and the Council of South African Trade Unions (COSATU), on 28 January 2002 three TAC members returned to South Africa from Brazil carrying generic versions of the antiretroviral drugs zidovudine (AZT), lamivudine (3TC), and nevirapine (NVP). Some of the imported capsules contain a combination of AZT and 3TC.

  11. Development of Refractories for Continuous Casting

    Institute of Scientific and Technical Information of China (English)

    TIAN Shouxin; JIN Congjin; YAO Jinfu; LI Zeya

    2004-01-01

    The paper introduces refractories for continuous casting, especially, refractories for continuous casting for clean steel in baosteel. Developing direction of refractories for continuous casting has been pointed out to satisfy the new metallurgical operating practice.

  12. Continuation-Passing C, compiling threads to events through continuations

    CERN Document Server

    Kerneis, Gabriel

    2010-01-01

    In this paper, we introduce Continuation Passing C (CPC), a programming language for concurrent systems in which native and cooperative threads are unified and presented to the programmer as a single abstraction. The CPC compiler uses a compilation technique, based on the CPS transform, that yields efficient code and an extremely lightweight representation for contexts. We provide a complete proof of the correctness of our compilation scheme. We show in particular that lambda-lifting, a common compilation technique for functional languages, is also correct in an imperative language like C, under some conditions enforced by the CPC compiler. The current CPC compiler is mature enough to write substantial programs such as Hekate, a highly concurrent BitTorrent seeder. Our benchmark results show that CPC is as efficient, while significantly cheaper, as the most efficient thread libraries available.

  13. Continuous Precipitation of Ceria Nanoparticles from a Continuous Flow Micromixer

    Energy Technology Data Exchange (ETDEWEB)

    Tseng, Chih Heng; Paul, Brian; Chang, Chih-hung; Engelhard, Mark H.

    2013-01-01

    Cerium oxide nanoparticles were continuously precipitated from a solution of cerium(III) nitrate and ammonium hydroxide using a micro-scale T-mixer. Findings show that the method of mixing is important in the ceria precipitation process. In batch mixing and deposition, disintegration and agglomeration dominates the deposited film. In T-mixing and deposition, more uniform nanorod particles are attainable. In addition, it was found that the micromixing approach reduced the exposure of the Ce(OH)3 precipates to oxygen, yielding hydroxide precipates in place of CeO2 precipitates. Advantages of the micro-scale T-mixing approach include shorter mixing times, better control of nanoparticle shape and less agglomeration.

  14. Continuous auditing: A literature review

    Directory of Open Access Journals (Sweden)

    Flávia Cruz de Souza

    2008-09-01

    Full Text Available Recent fraud scandals involving highly known corporations like Enron, WorldCom, and Xerox have eroded public confidence in financial reporting. At the same time, the auditing profession has suffered a big hit. In this scenario, Continuous Auditing (CA seems to have emerged as a response to recover the credibility of the auditing profession as well as meeting Sarbanes-Oxley (SOX requirements. This study, that adopts an exploratory approach, analyzes the existing literature on this topic. First, we address the concepts, models and implications of CA. After, throughout a search on the journals indexed at the CAPES's Basis, a literature review is conducted. A total of 57 articles were selected. We analyze authorship, affiliation, publications, year and type of research of papers that have addressed CA. Findings evidence that most articles addressing CA are non-empirical and adopt a conceptual approach. Also, there is an increasing tendency of continuous auditing studies. Rutgers University seems to be the World's leading research center on CA. This study aims to contribute to the Accounting Science by evidencing possibilities for research and publication in CA.

  15. Continuity properties of Moore cohomology

    CERN Document Server

    Austin, Tim

    2010-01-01

    In an important sequence of papers, Calvin Moore developed a version of group cohomology for locally compact groups taking into account their topology. He was able to re-establish most of the standard algebraic properties of group cohomology in the category of Polish Abelian modules for such groups, building initially on a bar resolution restricted to Borel cochains. However, the resulting cohomology groups can have rather unwieldy topological properties, and it remained mostly unclear whether they behave well under forming inverse limits of a sequence of base groups. This paper establishes that they are indeed continuous for such inverse limits for compact base groups and for a range of `nice' target modules, including all Banach spaces, discrete Abelian groups and tori when these are endowed with the trivial group action. In addition, this allows an extension of some results of Wigner identifying Moore's cohomology as isomorphic to alternatives constructed by other means, such as the Cech cohomology of the ...

  16. Congenital syphilis: The continuing scourge.

    Science.gov (United States)

    Agrawal, Prachi G; Joshi, Rajesh; Kharkar, Vidya D; Bhaskar, M V

    2014-01-01

    Congenital syphilis is a severe, disabling infection that occurs due to the transmission of Treponema pallidum across the placenta during pregnancy or from contact with an infectious genital lesion during delivery. However, its early diagnosis is often difficult because more than half of the affected infants are asymptomatic, and the signs in symptomatic infants may be subtle and nonspecific. Although its incidence is declining, this long-forgotten disease continues to affect pregnant women, resulting in considerable perinatal morbidity and mortality. We hereby report a case of a 2-month-old infant with early congenital syphilis presenting with joint swellings and Parrot's pseudoparalysis, a comparative rarity in the present scenario. The report also stresses upon the importance of implementing the Centres for Disease Control and Prevention recommendation that all the pregnant women should be screened for syphilis in the first antenatal visit in the first trimester and again in late pregnancy.

  17. Continuous improvement of software quality

    Energy Technology Data Exchange (ETDEWEB)

    Sivertsen, Terje

    1999-04-15

    The present report is the first Halden Work Report delivered from the OECD Halden Reactor Project's research activity on formal methods and software quality. Of particular concern in this activity is to reach a consensus between regulators, licensees and the nuclear industry on questions related to the effective, industrial use of formal methods. The report gives considerable attention to the importance of continuous improvement as a characteristic of a living software quality system, and to the need of providing a basis for software process/product quality integration. In particular, the report discusses these aspects from the perspectives of defect prevention, formal methods, Total Quality Management (TQM), and Bayesian Belief Nets. Another concern is to promote controlled experiments on the use of new methods, techniques, and tools. This is achieved partly by reviewing suggestions on the collection and experimental use of data, and by surveying a number of metrics believed to have some potential for comparison studies (author) (ml)

  18. Continuous Chemistry in Ice Cores

    DEFF Research Database (Denmark)

    Kjær, Helle Astrid

    originating from volcanic eruptions, crucial for cross-dating ice cores and relevant for climate interpretations. The method includes a heat bath to minimize the acidifying effect of CO2 both from the laboratory and from the ice itself. While for acidic ice the method finds similar concentrations of H......Ice cores provide high resolution records of past climate and environment. In recent years the use of continuous flow analysis (CFA) systems has increased the measurement throughput, while simultaneously decreasing the risk of contaminating the ice samples. CFA measurements of high temporal...... resolution increase our knowledge on fast climate variations and cover a wide range of proxies informing on a variety of components such as atmospheric transport, volcanic eruptions, forest fires and many more. New CFA methods for the determination of dissolved reactive phosphorus (DRP) and pH are presented...

  19. Continuing Professional Development: Best Practices

    Science.gov (United States)

    Filipe, Helena P.; Silva, Eduardo D.; Stulting, Andries A.; Golnik, Karl C.

    2014-01-01

    Continuing professional development (CPD) involves not only educational activities to enhance medical competence in medical knowledge and skills, but also in management, team building, professionalism, interpersonal communication, technology, teaching, and accountability. This paper aims at reviewing best practices to promote effective CPD. Principles and guidelines, as already defined by some professional societies and world organizations, are emphasized as core actions to best enhance an effective lifelong learning after residency. The personal learning plan (PLP) is discussed as the core of a well-structured CPD and we describe how it should be created. Fundamental CPD principles and how they are integrated in the framework of every physician's professional life will be described. The value of systematic and comprehensive CPD documentation and assessment is emphasized. Accreditation requirements and professional relationships with commercial sponsors are discussed. PMID:24791104

  20. 7 CFR 1160.117 - Continuation referendum.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 9 2010-01-01 2009-01-01 true Continuation referendum. 1160.117 Section 1160.117 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... Order Definitions § 1160.117 Continuation referendum. Continuation referendum means that referendum...

  1. Methods of producing continuous boron carbide fibers

    Energy Technology Data Exchange (ETDEWEB)

    Garnier, John E.; Griffith, George W.

    2015-12-01

    Methods of producing continuous boron carbide fibers. The method comprises reacting a continuous carbon fiber material and a boron oxide gas within a temperature range of from approximately 1400.degree. C. to approximately 2200.degree. C. Continuous boron carbide fibers, continuous fibers comprising boron carbide, and articles including at least a boron carbide coating are also disclosed.

  2. 40 CFR 1065.150 - Continuous sampling.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 32 2010-07-01 2010-07-01 false Continuous sampling. 1065.150 Section... ENGINE-TESTING PROCEDURES Equipment Specifications § 1065.150 Continuous sampling. You may use continuous sampling techniques for measurements that involve raw or dilute sampling. Make sure continuous...

  3. 42 CFR 441.60 - Continuing care.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 4 2010-10-01 2010-10-01 false Continuing care. 441.60 Section 441.60 Public... Periodic Screening, Diagnosis, and Treatment (EPSDT) of Individuals Under Age 21 § 441.60 Continuing care. (a) Continuing care provider. For purposes of this subpart, a continuing care provider means...

  4. Contra continuity and almost contra continuity in generalized fuzzy topological spaces

    Science.gov (United States)

    Bhattacharya, Baby; Chakraborty, Jayasree

    2015-05-01

    In this paper we introduce fuzzy contra continuity and almost contra continuity in generalized fuzzy topological space. Fuzzy almost contra continuity is weaker than fuzzy contra continuity in generalized fuzzy topological space. Then we investigate their characterizations and properties. We also established some equivalent relation on fuzzy contra continuity and fuzzy almost contra continuity in generalized fuzzy topological spaces.

  5. Temporal yoking in continuous multitasking.

    Science.gov (United States)

    Jiang, Yuhong V; Swallow, Khena M

    2014-12-01

    Continuous tasks such as baggage screening often involve selective gating of sensory information when "targets" are detected. Previous research has shown that temporal selection of behaviorally relevant information triggers changes in perception, learning, and memory. However, it is unclear whether temporal selection has broad effects on concurrent tasks. To address this question, we asked participants to view a stream of faces and encoded faces of a particular gender for a later memory test. At the same time, they listened to a sequence of tones, pressing a button for specific pitched tones. We manipulated the timing of temporal selection such that target faces and target tones could be unrelated, perfectly correlated, or anticorrelated. Temporal selection was successful when the temporally coinciding stimuli were congruent (e.g., both were targets), but not when they were incongruent (i.e., only 1 was a target). This pattern suggests that attentional selection for separate tasks is yoked in time-when the attentional gate opens for 1 task it also opens for the other. Temporal yoking is a unique form of dual-task interaction.

  6. Continued fractions and heavy sequences

    CERN Document Server

    Boshernitzan, Michael

    2009-01-01

    We initiate the study of the sets $H(c)$, $0=x-[x]$ stands for the fractional part of $x\\in \\mathbb R$. We prove that, for rational $c$, the sets $H(c)$ are of positive Hausdorff dimension and, in particular, are uncountable. For integers $m\\geq1$, we obtain a surprising characterization of the numbers $\\alpha\\in H_m= H(\\frac1m)$ in terms of their continued fraction expansions: The odd entries (partial quotients) of these expansions are divisible by $m$. The characterization implies that $x\\in H_m$ if and only if $\\frac 1{mx} \\in H_m$, for $x>0$. We are unaware of a direct proof of this equivalence, without making a use of the mentioned characterization of the sets $H_m$. We also introduce the dual sets $\\hat H_m$ of reals $y$ for which the sequence of integers $\\big([ky]\\big)_{k\\geq1}$ consistently hits the set $m\\mathbb Z$ with the at least expected frequency $\\frac1m$ and establish the connection with the sets $H_m$: {2mm} If $xy=m$ for $x,y>0$, then $x\\in H_m$ if and only if $y\\in \\hat H_m$. The motivatio...

  7. Genomic continuity of Argentinean Mennonites

    Science.gov (United States)

    Pardo-Seco, Jacobo; Llull, Cintia; Berardi, Gabriela; Gómez, Andrea; Andreatta, Fernando; Martinón-Torres, Federico; Toscanini, Ulises; Salas, Antonio

    2016-01-01

    Mennonites are Anabaptist communities that originated in Central Europe about 500 years ago. They initially migrated to different European countries, and in the early 18th century they established their first communities in North America, from where they moved to other American regions. We aimed to analyze an Argentinean Mennonite congregation from a genome-wide perspective by way of investigating >580.000 autosomal SNPs. Several analyses show that Argentinean Mennonites have European ancestry without signatures of admixture with other non-European American populations. Among the worldwide datasets used for population comparison, the CEU, which is the best-subrogated Central European population existing in The 1000 Genome Project, is the dataset showing the closest genome affinity to the Mennonites. When compared to other European population samples, the Mennonites show higher inbreeding coefficient values. Argentinean Mennonites show signatures of genetic continuity with no evidence of admixture with Americans of Native American or sub-Saharan African ancestry. Their genome indicates the existence of an increased endogamy compared to other Europeans most likely mirroring their lifestyle that involve small communities and historical consanguineous marriages. PMID:27824108

  8. Continuous sampling from distributed streams

    DEFF Research Database (Denmark)

    Graham, Cormode; Muthukrishnan, S.; Yi, Ke

    2012-01-01

    A fundamental problem in data management is to draw and maintain a sample of a large data set, for approximate query answering, selectivity estimation, and query planning. With large, streaming data sets, this problem becomes particularly difficult when the data is shared across multiple distribu......A fundamental problem in data management is to draw and maintain a sample of a large data set, for approximate query answering, selectivity estimation, and query planning. With large, streaming data sets, this problem becomes particularly difficult when the data is shared across multiple...... distributed sites. The main challenge is to ensure that a sample is drawn uniformly across the union of the data while minimizing the communication needed to run the protocol on the evolving data. At the same time, it is also necessary to make the protocol lightweight, by keeping the space and time costs low...... for each participant. In this article, we present communication-efficient protocols for continuously maintaining a sample (both with and without replacement) from k distributed streams. These apply to the case when we want a sample from the full streams, and to the sliding window cases of only the W most...

  9. Wide area continuous offender monitoring

    Energy Technology Data Exchange (ETDEWEB)

    Hoshen, J. [Lucent Technologies (United States); Drake, G. [New Mexico Dept. of Corrections, Santa Fe, NM (United States); Spencer, D. [Sandia National Labs., Albuquerque, NM (United States)

    1996-11-01

    The corrections system in the U.S. is supervising over five million offenders. This number is rising fast and so are the direct and indirect costs to society. To improve supervision and reduce the cost of parole and probation, first generation home arrest systems were introduced in 1987. While these systems proved to be helpful to the corrections system, their scope is rather limited because they only cover an offender at a single location and provide only a partial time coverage. To correct the limitations of first-generation systems, second-generation wide area continuous electronic offender monitoring systems, designed to monitor the offender at all times and locations, are now on the drawing board. These systems use radio frequency location technology to track the position of offenders. The challenge for this technology is the development of reliable personal locator devices that are small, lightweight, with long operational battery life, and indoors/outdoors accuracy of 100 meters or less. At the center of a second-generation system is a database that specifies the offender`s home, workplace, commute, and time the offender should be found in each. The database could also define areas from which the offender is excluded. To test compliance, the system would compare the observed coordinates of the offender with the stored location for a given time interval. Database logfiles will also enable law enforcement to determine if a monitored offender was present at a crime scene and thus include or exclude the offender as a potential suspect.

  10. Metabolic effects of continuous feeding.

    Science.gov (United States)

    Wolever, T M

    1990-09-01

    To study the metabolic effects of slowing absorption, as a possible mechanism for the blood glucose and lipid-lowering effects of soluble fiber and low glycemic index (GI) foods, seven healthy men consumed a liquid formula diet either as three equal meals at 4-hour intervals, or by continuously sipping the same amount of formula over the 12-hour study period. Meal-related fluctuations of blood glucose, insulin, and triglycerides were seen during three meals, but not during sipping. Mean 12-hour levels of blood glucose and beta-hydroxybutyrate (beta OHB) were equivalent on sipping and three meals. The total integrated insulin area was reduced by 32% on sipping (P less than .01), but this was not explained by the 16% (NS) reduction in serum C-peptide response. Mean serum free fatty acid (FFA) and cholesterol levels were reduced by 20% and 2.6%, respectively (P less than .01). It is concluded that the reduced glycemic responses seen after soluble fiber-enriched meals and low GI foods can be explained by slow absorption. Although the overall mean daily blood glucose levels may not be reduced by slowing carbohydrate absorption in nondiabetic subjects, this is achieved at considerably lower ambient serum insulin concentrations. The reduction of insulin levels may be an important mechanism for the serum cholesterol-lowering effect of soluble fiber and low GI foods.

  11. Continuously Connected With Mobile IP

    Science.gov (United States)

    2002-01-01

    Cisco Systems developed Cisco Mobile Networks, making IP devices mobile. With this innovation, a Cisco router and its connected IP devices can roam across network boundaries and connection types. Because a mobile user is able to keep the same IP address while roaming, a live IP connection can be maintained without interruption. Glenn Research Center jointly tested the technology with Cisco, and is working to use it on low-earth-orbiting research craft. With Cisco's Mobile Networks functionality now available in Cisco IOS Software release 12.2(4)T, the commercial advantages and benefits are numerous. The technology can be applied to public safety, military/homeland security, emergency management services, railroad and shipping systems, and the automotive industry. It will allow ambulances, police, firemen, and the U.S. Coast Guard to stay connected to their networks while on the move. In the wireless battlefield, the technology will provide rapid infrastructure deployment for U.S. national defense. Airline, train, and cruise passengers utilizing Cisco Mobile Networks can fly all around the world with a continuous Internet connection. Cisco IOS(R) Software is a registered trademark of Cisco Systems.

  12. Business and Continuity of Operations

    Science.gov (United States)

    Tosh, Pritish K.; Feldman, Henry; Christian, Michael D.; Devereaux, Asha V.; Kissoon, Niranjan; Christian, Michael D.; Devereaux, Asha V.; Dichter, Jeffrey R.; Kissoon, Niranjan; Rubinson, Lewis; Amundson, Dennis; Anderson, Michael R.; Balk, Robert; Barfield, Wanda D.; Bartz, Martha; Benditt, Josh; Beninati, William; Berkowitz, Kenneth A.; Daugherty Biddison, Lee; Braner, Dana; Branson, Richard D; Burkle, Frederick M.; Cairns, Bruce A.; Carr, Brendan G.; Courtney, Brooke; DeDecker, Lisa D.; De Jong, Marla J.; Dominguez-Cherit, Guillermo; Dries, David; Einav, Sharon; Erstad, Brian L.; Etienne, Mill; Fagbuyi, Daniel B.; Fang, Ray; Feldman, Henry; Garzon, Hernando; Geiling, James; Gomersall, Charles D.; Grissom, Colin K.; Hanfling, Dan; Hick, John L.; Hodge, James G.; Hupert, Nathaniel; Ingbar, David; Kanter, Robert K.; King, Mary A.; Kuhnley, Robert N.; Lawler, James; Leung, Sharon; Levy, Deborah A.; Lim, Matthew L.; Livinski, Alicia; Luyckx, Valerie; Marcozzi, David; Medina, Justine; Miramontes, David A.; Mutter, Ryan; Niven, Alexander S.; Penn, Matthew S.; Pepe, Paul E.; Powell, Tia; Prezant, David; Reed, Mary Jane; Rich, Preston; Rodriquez, Dario; Roxland, Beth E.; Sarani, Babak; Shah, Umair A.; Skippen, Peter; Sprung, Charles L.; Subbarao, Italo; Talmor, Daniel; Toner, Eric S.; Tosh, Pritish K.; Upperman, Jeffrey S.; Uyeki, Timothy M.; Weireter, Leonard J.; West, T. Eoin; Wilgis, John; Ornelas, Joe; McBride, Deborah; Reid, David; Baez, Amado; Baldisseri, Marie; Blumenstock, James S.; Cooper, Art; Ellender, Tim; Helminiak, Clare; Jimenez, Edgar; Krug, Steve; Lamana, Joe; Masur, Henry; Mathivha, L. Rudo; Osterholm, Michael T.; Reynolds, H. Neal; Sandrock, Christian; Sprecher, Armand; Tillyard, Andrew; White, Douglas; Wise, Robert; Yeskey, Kevin

    2014-01-01

    BACKGROUND: During disasters, supply chain vulnerabilities, such as power, transportation, and communication, may affect the delivery of medications and medical supplies and hamper the ability to deliver critical care services. Disasters also have the potential to disrupt information technology (IT) in health-care systems, resulting in interruptions in patient care, particularly critical care, and other health-care business functions. The suggestions in this article are important for all of those involved in a large-scale pandemic or disaster with multiple critically ill or injured patients, including front-line clinicians, hospital administrators, and public health or government officials. METHODS: The Business and Continuity of Operations Panel followed the American College of Chest Physicians (CHEST) Guidelines Oversight Committee’s methodology in developing key questions regarding medication and supply shortages and the impact disasters may have on healthcare IT. Task force members met in person to develop the 13 key questions believed to be most relevant for Business and Continuity of Operations. A systematic literature review was then performed for relevant articles and documents, reports, and gray literature reported since 2007. No studies of sufficient quality were identified upon which to make evidence-based recommendations. Therefore, the panel developed expert opinion-based suggestions using a modified Delphi process. RESULTS: Eighteen suggestions addressing mitigation strategies for supply chain vulnerabilities including medications and IT were generated. Suggestions offered to hospitals and health system leadership regarding medication and supply shortages include: (1) purchase key medications and supplies from more than one supplier, (2) substituted medications or supplies should ideally be similar to those already used by an institution’s providers, (3) inventories should be tracked electronically to monitor medication/supply levels, (4) consider

  13. Continuous profiling of magnetotelluric fields

    Energy Technology Data Exchange (ETDEWEB)

    Verdin, Carlos Torres [Univ. of California, Berkeley, CA (United States)

    1991-05-01

    The magnetotelluric (MT) method of mapping ground electrical conductivity is traditionally based on measurement of the surface impedance at widely spaced stations to infer models of the subsurface through a suitable pseudo 1-D inverse or with linearized least-squares inversion for 2- or 3-D geoelectric media. It is well known that small near-surface inhomogeneities can produce spatial discontinuities in the measured electric fields over a wide frequency range and may consequently bias the impedance on a very local scale. Inadequate station spacing effectively aliases the electric field measurements and results in distortions that cannot be removed in subsequent processing or modelling. In order to fully exploit the benefits of magnetotellurics in complex geological environments, closely spaced measurements must be used routinely. This thesis entertains an analysis of MT data taken along continuous profiles and is a first step that will allow more encompassing 2-D sampling techniques to become viable in the years to come. The developments presented here are to a large extent motivated by the physical insight gained from low-contrast solutions to the forward MT problem. These solutions describe the relationship between a perturbation in the electrical conductivity of the subsurface and the ensuing perturbation of the MT response as the output of a linear system. Albeit strictly accurate in a limited subset of practical exploration problems, the linearized solutions allow one to pursue a model independent study of the response characteristics of MT data. In fact, these solutions yield simple expressions for 1-,2-, and 3-D resistivity models which are here examined in progressive sequence.

  14. Continuous profiling of magnetotelluric fields

    Energy Technology Data Exchange (ETDEWEB)

    Verdin, C.T.

    1991-05-01

    The magnetotelluric (MT) method of mapping ground electrical conductivity is traditionally based on measurement of the surface impedance at widely spaced stations to infer models of the subsurface through a suitable pseudo 1-D inverse or with linearized least-squares inversion for 2- or 3-D geoelectric media. It is well known that small near-surface inhomogeneities can produce spatial discontinuities in the measured electric fields over a wide frequency range and may consequently bias the impedance on a very local scale. Inadequate station spacing effectively aliases the electric field measurements and results in distortions that cannot be removed in subsequent processing or modelling. In order to fully exploit the benefits of magnetotellurics in complex geological environments, closely spaced measurements must be used routinely. This thesis entertains an analysis of MT data taken along continuous profiles and is a first step that will allow more encompassing 2-D sampling techniques to become viable in the years to come. The developments presented here are to a large extent motivated by the physical insight gained from low-contrast solutions to the forward MT problem. These solutions describe the relationship between a perturbation in the electrical conductivity of the subsurface and the ensuing perturbation of the MT response as the output of a linear system. Albeit strictly accurate in a limited subset of practical exploration problems, the linearized solutions allow one to pursue a model independent study of the response characteristics of MT data. In fact, these solutions yield simple expressions for 1-,2-, and 3-D resistivity models which are here examined in progressive sequence.

  15. Opportunities of Continuing Adult Education

    Directory of Open Access Journals (Sweden)

    Lidija Ušeckienė

    2011-04-01

    Full Text Available After becoming the member state of the European Union, Lithuania undertook all the obligations of a member state. One of them is the implementation of The Lisbon Strategy aiming at the worlds most dynamic and competitive knowledge– based economy by 2010. Under the strategy, a stronger economy will drive job creation, sustainable development, and social inclusion. These changes demand the modernisation of education systems in the E U states, Lithuania among them. To achieve this objective, political forces came to an agreement on the future of Lithuanian education. In 2003 The Seimas of the Republic of Lithuania approved of National Education Strategy 2003–2012. This strategy is special not only because it is based on the experiences of the reform, addresses current and future world’s challenges and opportunities, maintains links with other strategic national reforms, but also emphasises efforts to ensure quality lifelong education for Lithuanian population and striving to become a partner in modern knowledge-based economy. Therefore, an extensive discussion on lifelong education strategies on individual and institution levels in all spheres of social and personal life has started in the E U and Lithuania. Nowadays lifelong learning is not just one aspect of education and training; it gradually is becoming the most important principle in the continuum of complex learning contexts. Such vision must be implemented this decade. The object of the research: the preconditions for the development of continuing adult education. The aim of the research: to examine the peculiarities of the preconditions for the development of continuing adult education in Pakruojis region. The methods of the research: analysis of references and documents on education; an anonymous survey in written form (a questionnaire; statistical analysis of data. The sample. The research was conducted in Pakruojis region in January-April, 2006. 300 respondents of different age

  16. Mercury Continuous Emmission Monitor Calibration

    Energy Technology Data Exchange (ETDEWEB)

    John Schabron; Eric Kalberer; Ryan Boysen; William Schuster; Joseph Rovani

    2009-03-12

    Mercury continuous emissions monitoring systems (CEMs) are being implemented in over 800 coal-fired power plant stacks throughput the U.S. Western Research Institute (WRI) is working closely with the Electric Power Research Institute (EPRI), the National Institute of Standards and Technology (NIST), and the Environmental Protection Agency (EPA) to facilitate the development of the experimental criteria for a NIST traceability protocol for dynamic elemental mercury vapor calibrators/generators. These devices are used to calibrate mercury CEMs at power plant sites. The Clean Air Mercury Rule (CAMR) which was published in the Federal Register on May 18, 2005 and vacated by a Federal appeals court in early 2008 required that calibration be performed with NIST-traceable standards. Despite the vacature, mercury emissions regulations in the future will require NIST traceable calibration standards, and EPA does not want to interrupt the effort towards developing NIST traceability protocols. The traceability procedures will be defined by EPA. An initial draft traceability protocol was issued by EPA in May 2007 for comment. In August 2007, EPA issued a conceptual interim traceability protocol for elemental mercury calibrators. The protocol is based on the actual analysis of the output of each calibration unit at several concentration levels ranging initially from about 2-40 {micro}g/m{sup 3} elemental mercury, and in the future down to 0.2 {micro}g/m{sup 3}, and this analysis will be directly traceable to analyses by NIST. The EPA traceability protocol document is divided into two separate sections. The first deals with the qualification of calibrator models by the vendors for use in mercury CEM calibration. The second describes the procedure that the vendors must use to certify the calibrators that meet the qualification specifications. The NIST traceable certification is performance based, traceable to analysis using isotope dilution inductively coupled plasma

  17. El Nino Continues to Grow

    Science.gov (United States)

    2002-01-01

    The latest image from NASA's Jason oceanography satellite, taken during a 10-day collection cycle ending December 2, 2002, shows the Pacific dominated by two significant areas of higher-than-normal sealevel (warmer ocean temperatures). In the central equatorial Pacific, the large area of higher than normal sea surface heights(warmer than normal sea surface temperatures) associated with growing El Nino conditions has recently migrated eastward toward the coast of South America. Meanwhile, the influence of the 20- to 30-year larger than El Nino/La Nina pattern called the Pacific Decadal Oscillation continues to create warm, higher-than-normal sea-surface heights in the north Pacific that are connected in a warm horseshoe pattern with the western and southern Pacific. Sea-surface heights are a measure of how much heat is stored in the ocean below. This heat influences both present weather and future planetary climate events.The image shows red areas in the north Pacific and at the equator that are about 10 centimeters (4 inches) above normal; white areas indicate sea surface heights between 14 and 32 centimeters (6 to 13 inches) above normal. These regions contrast with the western tropical Pacific, where lower-than-normal sea levels (blue areas) have developed that are between 5 and 13 centimeters (2 and 5 inches) below normal, while purple areas range from 14 to 18 centimeters (6 to 7 inches) below normal. Along the equator, the red sea surface heights equate to sea surface temperature departures greater than one degree Celsius (two degrees Fahrenheit) and the white sea surface heights are sea surface temperatures 1.5 to 2.5 degrees Celsius(three to five degrees Fahrenheit) above normal.The U.S. portion of the Jason mission is managed by JPL for NASA's Earth Science Enterprise, Washington, D.C. Research on Earth's oceans using Jason and other space-based capabilities is conducted by NASA's Earth Science Enterprise to better understand and protect our home planet.

  18. Continuous EEG in Critically Ill Children

    Directory of Open Access Journals (Sweden)

    Jonathan E Kurz

    2015-03-01

    Full Text Available Investigators from the Critical Care Continuous EEG Task Force of the American Clinical Neurophysiology Society reported a consensus statement on indications for the use of critical care continuous electroencephalographic monitoring (ccEEG in adults and children.

  19. Transcendental $p$-adic continued fractions

    OpenAIRE

    Ooto, Tomohiro

    2014-01-01

    We establish a new transcendence criterion of $p$-adic continued fractions which are called Ruban continued fractions. By this result, we give explicit transcendental Ruban continued fractions with bounded $p$-adic absolute value of partial quotients. This is $p$-adic analogy of Baker's result. We also prove that $p$-adic analogy of Lagrange Theorem for Ruban continued fractions is not true.

  20. Effectiveness of continuing medical education.

    Science.gov (United States)

    Marinopoulos, Spyridon S; Dorman, Todd; Ratanawongsa, Neda; Wilson, Lisa M; Ashar, Bimal H; Magaziner, Jeffrey L; Miller, Redonda G; Thomas, Patricia A; Prokopowicz, Gregory P; Qayyum, Rehan; Bass, Eric B

    2007-01-01

    OBJECTIVES Despite the broad range of continuing medical education (CME) offerings aimed at educating practicing physicians through the provision of up-to-date clinical information, physicians commonly overuse, under-use, and misuse therapeutic and diagnostic interventions. It has been suggested that the ineffective nature of CME either accounts for the discrepancy between evidence and practice or at a minimum contributes to this gap. Understanding what CME tools and techniques are most effective in disseminating and retaining medical knowledge is critical to improving CME and thus diminishing the gap between evidence and practice. The purpose of this review was to comprehensively and systematically synthesize evidence regarding the effectiveness of CME and differing instructional designs in terms of knowledge, attitudes, skills, practice behavior, and clinical practice outcomes. REVIEW METHODS We formulated specific questions with input from external experts and representatives of the Agency for Healthcare Research and Quality (AHRQ) and the American College of Chest Physicians (ACCP) which nominated this topic. We systematically searched the literature using specific eligibility criteria, hand searching of selected journals, and electronic databases including: MEDLINE, EMBASE, the Cochrane Database of Systematic Reviews, The Cochrane Central Register of Controlled Trials (CENTRAL), the Cochrane Database of Abstracts of Reviews of Effects (DARE), PsycINFO, and the Educational Resource Information Center (ERIC). Two independent reviewers conducted title scans, abstract reviews, and then full article reviews to identify eligible articles. Each eligible article underwent double review for data abstraction and assessment of study quality. RESULTS Of the 68,000 citations identified by literature searching, 136 articles and 9 systematic reviews ultimately met our eligibility criteria. The overall quality of the literature was low and consequently firm conclusions were

  1. [Relation between continuity of care and mortality

    NARCIS (Netherlands)

    Uijen, A.A.; Schers, H.J.

    2017-01-01

    Continuity of care is one of the most important features of primary care. In this commentary we discuss the article by Maarsingh et al. regarding the relation between personal continuity (seeing the same care provider) and mortality. We raise some important points on the way continuity of care is

  2. Bayesian belief networks in business continuity

    NARCIS (Netherlands)

    Phillipson, F.; Matthijssen, E.F.; Attema, T.

    2014-01-01

    Business continuity professionals try to deal with multiple aspects which influence the continuity of a company. The key product is a coherent system of measures which encompass detection, prevention and recovery. Choices made in one element of the system affect other elements as well as the continu

  3. Security Support in Continuous Deployment Pipeline

    DEFF Research Database (Denmark)

    Ullah, Faheem; Raft, Adam Johannes; Shahin, Mojtaba

    2017-01-01

    Continuous Deployment (CD) has emerged as a new practice in the software industry to continuously and automatically deploy software changes into production. Continuous Deployment Pipeline (CDP) supports CD practice by transferring the changes from the repository to production. Since most of the C...

  4. 78 FR 21245 - Continuity of Operations Plan

    Science.gov (United States)

    2013-04-10

    ... Federal Energy Regulatory Commission 18 CFR Part 376 Continuity of Operations Plan AGENCY: Federal Energy... Continuity of Operations Plan regulations to revise its hierarchy of delegation of Commission authority... Rule revises the Commission's Continuity of Operations Plan (COOP) regulations to incorporate...

  5. Ant colony optimization in continuous problem

    Institute of Scientific and Technical Information of China (English)

    YU Ling; LIU Kang; LI Kaishi

    2007-01-01

    Based on the analysis of the basic ant colony optimization and optimum problem in a continuous space,an ant colony optimization (ACO) for continuous problem is constructed and discussed. The algorithm is efficient and beneficial to the study of the ant colony optimization in a continuous space.

  6. Continued fractions and the second Kepler law

    OpenAIRE

    Karpenkov, Oleg

    2009-01-01

    In this paper we introduce a link between geometry of ordinary continued fractions and trajectories of points that moves according to the second Kepler law. We expand geometric interpretation of ordinary continued fractions to the case of continued fractions with arbitrary elements.

  7. Semi-Continuity of Complex Fuzzy Functions

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    This paper introduces the concept of semi-continuity of complex fuzzy functions, and discusses some of their elementary properties, such as the sum of two complex fuzzy functions of type I upper (lower)semi-continuity is type I upper (lower) semi-continuous, and the opposite of complex fuzzy functions of type I upper (lower) semi-continuity is type I lower (upper) semi-continuous. Based on some assumptions on two complex fuzzy functions of type I upper (lower) semi-continuity, it is shown that their product is type I upper (lower) semi-continuous. The paper also investigates the convergence of complex fuzzy functions. In particular, sign theorem, boundedness theorem, and Cauchy's criterion for convergence are kept. In this paper the metrics introduced by Zhang Guangquan was used. This paper gives a contribution to the study of complex fuzzy functions, and extends the corresponding work of Zhang Guangquan.

  8. Statistical inference for critical continuous state and continuous time branching processes with immigration

    OpenAIRE

    2014-01-01

    We study asymptotic behavior of conditional least squares estimators for critical continuous state and continuous time branching processes with immigration based on discrete time (low frequency) observations.

  9. Internal factors influencing the knowledge continuity ensuring

    Directory of Open Access Journals (Sweden)

    Hana Urbancová

    2012-01-01

    Full Text Available The aim of the systematic ensuring of knowledge continuity is the continuity of an organisation’s development, the quality of managerial positions and the continuity of decision-making. By ensuring knowledge continuity, organisations may gain a performance-enhancing factor. The objective of the article is to identify the level of impact of decisive internal factors determining knowledge continuity ensuring and contributing to the efficiency of the organisations. Knowledge continuity ensuring as an internal force, however, can together with the right employees, help adapt more quickly to external conditions that organisations can hardly control. Monitoring and ensuring knowledge continuity can contribute to a higher quality of processes in general, in particular processes exploiting knowledge, and thus help improve the level of management. The first part of the article presents theoretical views on the aspects of knowledge continuity ensuring in organisations while the second part analyses the findings of the surveys carried out among managers in organisations in the Czech Republic. Based on the summary of the outcomes obtained it is possible to say that internal factors influence knowledge continuity ensuring in organisations, however, the level of impact of individual factors is determined by their size. The findings regarding the impact of each of the factors show that the most significant barriers to knowledge continuity ensuring are those associated with the human factor.

  10. Midwifery continuity: The use of social media.

    Science.gov (United States)

    McCarthy, Rose; Choucri, Lesley; Ormandy, Paula; Brettle, Alison

    2017-09-01

    Continuity models of midwifery care improve women's experiences of care and clinical outcomes, but organisationally driven working practices do not facilitate a continuity model and the midwifery care received by most women is fragmented (Sandall et al., 2016, NHS England, 2016). Little is known about the potential for continuity of midwifery care to be achieved using an electronic platform. This paper examines the experiences of women accessing known midwives through a social media platform and their experiences and perception of continuity of care. The study forms part of a larger research project aiming to increase understanding about online social learning within professionally moderated social media based communities. This paper reports specifically the concept of midwifery continuity within the online communities. Two secret Facebook groups consisting of 31 mothers and 4 midwife moderators were created (17 mothers & 2 midwives / 14 mothers & 2 midwives). Primary data included 8 online and face to face focus groups, conducted at approximately 10 week intervals, and 28 individual one to one interviews with members of the online community within six weeks of giving birth. A thematic analysis using a priori themes was undertaken. This involved coding data which evidenced relational, informational and management continuity across the entire dataset (28 interviews and 8 focus groups). The analysis was undertaken broadly following the six stages described by Braun and Clarke (2006). Relational and informational continuity were identified across the data. Relational continuity was evident for both the participants and the midwife moderators; informational continuity was described by the participants. Management continuity was not identified. Continuity through social media use was valued by both the mothers and the midwives. Information and relational continuity needs of women can be met using professionally moderated, social media based groups. They may

  11. Continuous Weight Attack on Complex Network

    Institute of Scientific and Technical Information of China (English)

    YIN Yan-Ping; ZHANG Duan-Ming; TAN Jin; PAN Gui-Jun; HE Min-Hua

    2008-01-01

    We introduce a continuous weight attack strategy and numerically investigate the effect of continuous use a weight coefficient ω to define the attack intensity. The weight coefficient ω increases continuously from 1 to infinity, where 1 represents no attack and infinity represents complete destructive attack. Our results show that the continuous weight attack on two selected nodes with small ω (ω≈ 3) could achieve the same damage of complete elimination of a single selected node on both BA and ER networks. It is found that the continuous weight attack on a single selected edge with small ω (ω≈ 2) can reach the same effect of complete elimination of a single edge on BA network, but on ER network the damage of the continuous weight attack on a single edge is close to but always smaller than that of complete elimination of edge even if ω is very large.

  12. Quantum engineering of continuous variable quantum states

    Energy Technology Data Exchange (ETDEWEB)

    Sabuncu, Metin

    2009-10-29

    Quantum information with continuous variables is a field attracting increasing attention recently. In continuous variable quantum information one makes use of the continuous information encoded into the quadrature of a quantized light field instead of binary quantities such as the polarization state of a single photon. This brand new research area is witnessing exciting theoretical and experimental achievements such as teleportation, quantum computation and quantum error correction. The rapid development of the field is mainly due higher optical data rates and the availability of simple and efficient manipulation tools in continuous-variable quantum information processing. We in this thesis extend the work in continuous variable quantum information processing and report on novel experiments on amplification, cloning, minimal disturbance and noise erasure protocols. The promising results we obtain in these pioneering experiments indicate that the future of continuous variable quantum information is bright and many advances can be foreseen. (orig.)

  13. Voltage stability, bifurcation parameters and continuation methods

    Energy Technology Data Exchange (ETDEWEB)

    Alvarado, F.L. [Wisconsin Univ., Madison, WI (United States)

    1994-12-31

    This paper considers the importance of the choice of bifurcation parameter in the determination of the voltage stability limit and the maximum power load ability of a system. When the bifurcation parameter is power demand, the two limits are equivalent. However, when other types of load models and bifurcation parameters are considered, the two concepts differ. The continuation method is considered as a method for determination of voltage stability margins. Three variants of the continuation method are described: the continuation parameter is the bifurcation parameter the continuation parameter is initially the bifurcation parameter, but is free to change, and the continuation parameter is a new `arc length` parameter. Implementations of voltage stability software using continuation methods are described. (author) 23 refs., 9 figs.

  14. Business continuity management in international organisations.

    Science.gov (United States)

    Adamou, Christel

    2014-01-01

    In the area of business continuity management, a preliminary review of the literature reveals extensive knowledge, expertise and experience concerning organisations in the private and public sectors. It is interesting to note, however, that there is little literature about business continuity management in international organisations, although these entities are complex and particularly prone to threats. This apparent absence of literature suggests that business continuity management has not yet hit the agenda of international organisations. In recent years, member states have encouraged senior management to design and implement business continuity strategies to minimise the mishandling of an internal crisis and build organisational resilience, but very few of them have actually been able to design and implement comprehensive business continuity programmes. Based on actual experience working in international organisations, this paper outlines some of the challenges faced by international organisations in developing and implementing business continuity activities and attempts to make suggestions for further improvement.

  15. Continuity and export performance in Peruvian companies

    Directory of Open Access Journals (Sweden)

    Oscar Malca Guaylupo

    2016-01-01

    Full Text Available This paper studies the relationship between continuity of business in international markets and their export performance. For this, the paper focuses on the Peruvian export companies during the period 2000-2008, stage where Peruvian exports had continued growth. the results achieved in international markets are analyzed and through probabilistic logit model establishes the relationship between continuity in these markets and the export performance measured in export sales.

  16. Business Continuity Planning for Risk Reduction

    OpenAIRE

    Andreea ZAMFIR; Delia TUDOR; PLUMB Ion

    2007-01-01

    The paper outlines the business continuity planning as a methodology that could be used by organizations in order to reduce the risks that occur both at the organizational level and in its outside environment. There are presented the main objectives and steps in business continuity planning process. In the end of the paper are presented some issues that organizations should take into consideration in the implementation of business continuity planning process projects.

  17. Bayesian belief networks in business continuity.

    Science.gov (United States)

    Phillipson, Frank; Matthijssen, Edwin; Attema, Thomas

    2014-01-01

    Business continuity professionals aim to mitigate the various challenges to the continuity of their company. The goal is a coherent system of measures that encompass detection, prevention and recovery. Choices made in one part of the system affect other parts as well as the continuity risks of the company. In complex organisations, however, these relations are far from obvious. This paper proposes the use of Bayesian belief networks to expose these relations, and presents a modelling framework for this approach.

  18. Retrospective turn continuations in Mandarin Chinese conversation

    OpenAIRE

    2007-01-01

    How the status of further talk past the point of a turn's possible completion should he described, and what functions different kinds of turn continuation might serve - these are questions that have engaged many scholars since Sacks, Schegloff and Jefferson's turn-taking model (1974). In this paper, a general scheme is proposed with which one can tease out four interlocking strands in analyzing different kinds of turn continuation: Syntactic continuity vs. discontinuity, main vs. subordinate ...

  19. Automatic penalty continuation in structural topology optimization

    DEFF Research Database (Denmark)

    Rojas Labanda, Susana; Stolpe, Mathias

    2015-01-01

    this issue is addressed. We propose an automatic continuation method, where the material penalization parameter is included as a new variable in the problem and a constraint guarantees that the requested penalty is eventually reached. The numerical results suggest that this approach is an appealing...... alternative to continuation methods. Automatic continuation also generally obtains better designs than the classical formulation using a reduced number of iterations....

  20. Totally semi-continuous and semi totally-continuous functions in double fuzzy topological spaces

    Science.gov (United States)

    Mahmood Mohammed, Fatimah; Md Noorani, Mohd Salmi; Salleh, Abdul Razak

    2013-04-01

    The aim of this paper is to introduce new classes of functions called totally-continuous functions and its variants totally semi-continuous functions and semi totally-continuous functions in double fuzzy topological spaces. Their characterizations, examples and relationship with other notions of continuous functions in this space are provided.

  1. Discrete continuous-phase superresolving filters.

    Science.gov (United States)

    Zhou, Sumei; Zhou, Changhe

    2004-12-01

    A new type of phase-only superresolving pupil filter with a discrete continuous-phase profile is presented that is a combination of discrete multilevel-phase modulation and continuous-phase modulation. This type of filter can achieve better superresolution performance than the continuous-phase filters reported in Opt. Lett. 28, 607 (2003). Therefore, with regard to the superresolution effect, this type of filter deserves study for practical applications. More importantly, the diffraction performance of this type of filter can explain the effect of a discrete-phase filter illuminated with a continuous wave front, whose superresolving performance cannot be analyzed with previous superresolution methods.

  2. Measuring business continuity programmes in large organisations.

    Science.gov (United States)

    Green, Christopher

    2014-01-01

    In the field of business continuity management, organisations commit sums of money (often very large sums) to develop and maintain their continuity capability. Despite this, there is almost no measurement of whether this expense offers value for money, or whether it is targeted in the right areas. This paper will explain some methods of measuring components of a business continuity programme. The important outputs from this measurement activity are to demonstrate that an organisation's continuity capability is improving over time, and to identify areas of weakness that should be targeted during future work.

  3. Hanford year 2000 Business Continuity Plan

    Energy Technology Data Exchange (ETDEWEB)

    ROGGENKAMP, S.L.

    1999-11-01

    The goal of Department of Energy Richland Operations (DOE-RL) Year 2000 (Y2K) effort is to ensure that the Hanford site successfully continues its mission as we approach and enter the 21th century. The Y2K Business Continuity Planning process provides a structured approach to identify Y2K risks to the site and to mitigate these risks through Y2K Contingency Planning, ''Zero-Day'' Transition Planning and Emergency Preparedness. This document defines the responsibilities, processes and plans for Hanford's Y2K Business Continuity. It identifies proposed business continuity drills, tentative schedule and milestones.

  4. Continuity of care: differing conceptions and values.

    Science.gov (United States)

    Delva, Dianne; Kerr, Jonathan; Schultz, Karen

    2011-08-01

    To understand how the conception of continuity of care can influence family physician trainees by exploring the perspectives of established family physicians, physicians working in episodic care who had been trained in family medicine, and family medicine trainees. Qualitative analysis of focus group data. Southeastern Ontario. Seven focus groups consisting of members from 3 groups: established family physicians, physicians working in episodic care who had been trained in family medicine, or family medicine trainees. Semistructured focus group interviews were taped and transcribed. Using constant comparison, the transcripts were analyzed for themes related to continuity of care and how these were valued among the 3 groups of physicians. The 3 groups differed on how they valued continuity of the relationship, how they valued informational continuity, and how these concepts affected their perceptions of difficult clinician-patient relationships. Experienced family physicians described long-term relationships as a core value in their practices. In contrast, episodic care physicians valued informational continuity. Family medicine trainees learned about continuity of care through role models and theoretical teaching. They valued the efficiency gained by knowing patients and the reward of being recognized by patients. Family medicine trainees expressed greater distress with difficult clinician-patient interactions than experienced family physicians expressed. It was unclear whether the challenges of difficult relationships were offset by the trainees' appreciation of continuity of care. Different perceptions, settings, and skills can influence how continuity of care is valued, which might affect career and practice decisions among trainees.

  5. Continuous Variable Quantum Communication and Computation

    DEFF Research Database (Denmark)

    Andersen, Ulrik Lund; Dong, Ruifang; Jezek, Miroslav

    2011-01-01

    We use squeezed states of light to implement a robust continuous variable quantum key distribution scheme and an optical Hadamard gate based on coherent state qubits.......We use squeezed states of light to implement a robust continuous variable quantum key distribution scheme and an optical Hadamard gate based on coherent state qubits....

  6. Continuous oxygen therapy for hypoxic pulmonary disease

    DEFF Research Database (Denmark)

    Ringbaek, Thomas J

    2005-01-01

    Continuous oxygen therapy (COT) has become widely accepted in the last 20 years in patients with continuous hypoxemia. This review focuses on guidelines for COT, adherence to these guidelines, and the effect of COT on survival, hospitalization, and quality of life. Guidelines for COT are mainly...

  7. Quest for Continual Growth Takes Root

    Science.gov (United States)

    Surdey, Mary M.; Hashey, Jane M.

    2006-01-01

    In this article, the authors describe how the quest for continual growth has taken its root at Vestal Central School district. Located at the heart of upstate New York, educators at Vestal Central School district have created a spirit of "kaizen," a Japanese word meaning the relentless quest for continual improvement and higher-quality…

  8. Left Baire-1 Compositors and Continuous Functions

    Directory of Open Access Journals (Sweden)

    Jonald P. Fenecios

    2013-01-01

    we provide a new proof that the class of functions ℬ, where is the class of all positive continuous functions and is the class of all positive constants as defined in the work by Bakowska and Pawlak (2010 is exactly the class of all continuous functions.

  9. Automatic Blind Syllable Segmentation for Continuous Speech

    OpenAIRE

    Villing, Rudi; Timoney, Joseph; Ward, Tomas

    2004-01-01

    In this paper a simple practical method for blind segmentation of continuous speech into its constituent syllables is presented. This technique which uses amplitude onset velocity and coarse spectral makeup to identify syllable boundaries is tested on a corpus of continuous speech and compared with an established segmentation algorithm. The results show substantial performance benefit using the proposed algorithm.

  10. A Strategy for Sourcing Continuing Engineering Education

    Science.gov (United States)

    Baukal, Charles E., Jr.

    2012-01-01

    Many are calling for increased continuing education for engineers, but few details are provided as to how to source that education. This paper recommends a strategy for sourcing continuing engineering education (CEE). Providers of CEE are categorized here as internal (the organization itself), external (universities, professional/trade…

  11. Standards for Continuing Education in Nursing.

    Science.gov (United States)

    American Nurses' Association, New York, NY.

    The quality of health care depends to a large degree on the knowledge, skills, and attitudes of practicing nurses. Continuing education is one way nurses can maintain competence and meet the standards of their profession. Continuing education in nursing consists of planned learning experiences beyond a basic nursing educational program. Providers…

  12. Nourishing Professional Practice: Continuing Education in Dietetics.

    Science.gov (United States)

    Kinneer, James W.

    The literature on continuing education (CE) in dietetics was reviewed. The review focused on the following: motivators and barriers for participation in continuing dietetic education, formats for CE in dietetics, and approaches to assessing learner needs. The role of professional associations, the existence of voluntary credentialing programs, the…

  13. Continuous Classroom Assessment at Primary Level

    Science.gov (United States)

    Ali, Imtiaz; Shah, Syed Manzoor Hussein; Gujjar, Aijaz Ahmed

    2014-01-01

    This study was designed to analyze the continuous classroom assessment at primary level in Pakistan. Findings of the study revealed that the students' achievement of single class teacher in the subject of English, General science, Urdu and mathematics were almost on average and rubric observation during continuous classroom assessment ranked…

  14. Continuous pain intensity estimation from facial expressions

    NARCIS (Netherlands)

    Kaltwang, Sebastian; Rudovic, Ognjen; Pantic, Maja

    2012-01-01

    Automatic pain recognition is an evolving research area with promising applications in health care. In this paper, we propose the first fully automatic approach to continuous pain intensity estimation from facial images. We first learn a set of independent regression functions for continuous pain in

  15. 29 CFR 825.115 - Continuing treatment.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Continuing treatment. 825.115 Section 825.115 Labor... treatment. A serious health condition involving continuing treatment by a health care provider includes any one or more of the following: (a) Incapacity and treatment. A period of incapacity of more than...

  16. Continuing Education Needs in Latin America.

    Science.gov (United States)

    Capanema, Clelia de Freitas

    Continuing education needs of the Latin American population are related to the need of Latin American countries for social and economic development. The source of continuing educaton needs is the area's differentiated socioeconomic development and cultural diversity. Some common features of Latin American educational systems, well known as…

  17. Continued fractions constructed from prime numbers

    CERN Document Server

    Wolf, Marek

    2010-01-01

    We give 50 digits values of the simple continued fractions whose denominators are formed from a) prime numbers, b) twin primes, c) primes of the form m^2+1 and Mersenne primes. All these continued fractions belong to the set of measure zero of exceptions to the Khinchin Theorem.

  18. Continuous pain intensity estimation from facial expressions

    NARCIS (Netherlands)

    Kaltwang, Sebastian; Rudovic, Ognjen; Pantic, Maja

    2012-01-01

    Automatic pain recognition is an evolving research area with promising applications in health care. In this paper, we propose the first fully automatic approach to continuous pain intensity estimation from facial images. We first learn a set of independent regression functions for continuous pain

  19. Review Articles for Continuing Education in Pharmacology.

    Science.gov (United States)

    Dionne, Raymond A.

    1983-01-01

    A study of 196 dentists' comprehension of an article in a leading dental continuing education journal revealed about four-fifths gaining knowledge, and the remainder decreasing or showing no change in knowledge. Use of scholarly articles as an efficient continuing education method is recommended. (MSE)

  20. Continuous selections and sigma-spaces

    CERN Document Server

    Repovs, Dusan; Zdomskyy, Lyubomyr

    2007-01-01

    Assume that X is a metrizable separable space, and each clopen-valued lower semicontinuous multivalued map Phi from X to Q has a continuous selection. Our main result is that in this case, X is a sigma-space. We also derive a partial converse implication, and present a reformulation of the Scheepers Conjecture in the language of continuous selections.

  1. A Marketing Management Approach for Continuing Education.

    Science.gov (United States)

    Taylor, Thomas E.

    1986-01-01

    Applies a marketing management model to the revitalization, or remarketing, of continuing education. Assesses the potential of continuing education as a higher education market. Suggests surveying present students, developing and quantifying hypotheses, applying new technology, promoting selected benefits, approaching areas of opportunity, and…

  2. Continuous-Reading Cryogen Level Sensor

    Science.gov (United States)

    Barone, F. E.; Fox, E.; Macumber, S.

    1984-01-01

    Two pressure transducers used in system for measuring amount of cryogenic liquid in tank. System provides continuous measurements accurate within 0.03 percent. Sensors determine pressure in liquid and vapor in tank. Microprocessor uses pressure difference to compute mass of cryogenic liquid in tank. New system allows continuous sensing; unaffected by localized variations in composition and density as are capacitance-sensing schemes.

  3. A Reflection on Continuing Professional Education Research

    Science.gov (United States)

    Brennan, Barrie

    2017-01-01

    Barrie Brennan's thesis entitled "Continuing Professional Education in Australia. A Tale of Missed Opportunities" offers a history of Continuing Professional Development (CPD) in the Australian context. This paper arose from Brennan's research for his thesis and is focused on issues that arose from the introduction of Australia's…

  4. Continuing Higher Education: The Coming Wave.

    Science.gov (United States)

    Lerner, Allan W., Ed.; King, B. Kay, Ed.

    This book, containing seven chapters and an epilogue, describes how continuing education--as a structure and a function--can become a unique tool for reorienting major universities toward confronting new societal challenges. "The Coming Wave" (Lerner) discusses relevant organizational theory to explain the special role of continuing education in…

  5. Continuing Education on Dying and Death.

    Science.gov (United States)

    Chodil, Judith J.; Dulaney, Peggy E.

    1984-01-01

    "Dying and Death in Critical Care Practice" was a one-day continuing education offering designed for registered nurses who practiced in settings such as emergency rooms, intensive care units, coronary care units, and operating rooms. The workshop was part of a continuing education curriculum in critical care nursing. (SSH)

  6. Strategy Planning in Continuing Nursing Education.

    Science.gov (United States)

    Aiken, Eula

    Corporate strategies that can be used in continuing nursing education programs are discussed, based on the Regional Action for Continuing Education in Nursing Education project. Attention is focused on strategies employed at Southern State University (SSU). A practical planning method was used to guide the process of corporate strategy formulation…

  7. 'The mother of all continued fractions'

    NARCIS (Netherlands)

    Dajani, K.; Kraaikamp, C.

    2001-01-01

    In this paper we give the relationship between the regular continued fraction and the Lehner fractions using a procedure known as insertion Starting from the regular continued fraction expansion of any real irrational x when the maximal number of insertions is applied one obtains the Lehner fraction

  8. Continuous hydrogen production from organic waste

    Energy Technology Data Exchange (ETDEWEB)

    Noike, T.; Li, Y.Y. [Tohoku Univ., Sendai (Japan). Dept. of Civil Engineering; Ko, I.B. [Cheju National Univ. Jeju (Korea, Republic of). Dept. of Environmental Engineering; Yokoyama, S. [Sanki Engineering CP., Ltd. (Japan); Kohno, Y. [Ex Corporation City and Environmental Planning, Research (Japan)

    2004-07-01

    Hydrogen is seen as a promising fuel for the future. Microbial fermentation of organic wastes is an attractive option. However, it has often been observed that hydrogen production in a continuously-fed reactor stops after a period of time. In this investigation, the effect of pH on lactic acid bacteria in continuous hydrogen production is studied. It was found that the antibiotic effect of the bacteria is prevented by keeping the pH greater than five. To establish what stops continuous hydrogen production, a continuous experiment was run using waste from bean curd manufacturing at a pH of 5.5 and a temperature of 35 degrees celsius. An increase in substrate concentration and the addition of nitrogen preferentially produced acetic and butyric acids and suppressed the production of propionic acid. Because of this, continuous hydrogen production was made possible. 4 refs., 5 tabs., 5 figs.

  9. Continuous C*-algebras over topological spaces

    CERN Document Server

    Takeori, Mitsuharu

    2010-01-01

    We define continuous C*-algebras over a topological space X and establish some basic results. If X is a locally compact Hausdorff space, continuous C*-algebras over X are equivalent to ordinary continuous C_0(X)-algebras. The main purpose of our study is to prove that every continuous, full, separable, nuclear C*-algebra over X is KK(X)-equivalent to a stable Kirchberg algebra over X. (Here a Kirchberg algebra over X is a separable, nuclear, and strongly purely infinite C*-algebra over X with primitive ideal space homeomorphic to X.) In the case that X is a one-point space, this result is known as that every separable nuclear C*-algebra is KK-equivalent to a stable Kirchberg algebra. Moreover, as an intermediate result, we obtain the X-equivariant exact embedding result for continuous C*-algebras over X.

  10. Extended Hamiltonian approach to continuous tempering.

    Science.gov (United States)

    Gobbo, Gianpaolo; Leimkuhler, Benedict J

    2015-06-01

    We introduce an enhanced sampling simulation technique based on continuous tempering, i.e., on continuously varying the temperature of the system under investigation. Our approach is mathematically straightforward, being based on an extended Hamiltonian formulation in which an auxiliary degree of freedom, determining the effective temperature, is coupled to the physical system. The physical system and its temperature evolve continuously in time according to the equations of motion derived from the extended Hamiltonian. Due to the Hamiltonian structure, it is easy to show that a particular subset of the configurations of the extended system is distributed according to the canonical ensemble for the physical system at the correct physical temperature.

  11. Building a global business continuity programme.

    Science.gov (United States)

    Lazcano, Michael

    2014-01-01

    Business continuity programmes provide an important function within organisations, especially when aligned with and supportive of the organisation's goals, objectives and organisational culture. Continuity programmes for large, complex international organisations, unlike those for compact national companies, are more difficult to design, build, implement and maintain. Programmes for international organisations require attention to structural design, support across organisational leadership and hierarchy, seamless integration with the organisation's culture, measured success and demonstrated value. This paper details practical, but sometimes overlooked considerations for building successful global business continuity programmes.

  12. PHYSICAL SIMULATION OF CONTINUOUS ROLL CASTING PROCESS

    Institute of Scientific and Technical Information of China (English)

    L.H. Zhan; J. Zhong; X.Q. Li; M.H. Huang

    2005-01-01

    A series of simulating experimental studies on the rheological behavior and its influential factors of aluminum alloy in continuous roll-casting process have been explored in this paper with a Gleeble-1500 Thermal-Mechanical Simulation Tester and a set of special clamp system. Relevant rheological rules in the process of coupling transient solidification and continuous deformation of roll-casting conditions are obtained. Experimental results indicate that four different characteristic stages exist in the whole rheological process, and relative constitutive models suitable for the given conditions of continuous roll casting process have been established through multivariable linear regression analysis of the experimental data.

  13. THE EQUATION FOR THE CONTINUOUS INGOT SOLIDIFICATION PROCESS FOR CONTROL SYSTEMS OF CONTINUOUS CASTING CASTER MNLZ

    Directory of Open Access Journals (Sweden)

    I. A. Pugachev

    2013-01-01

    Full Text Available A process of heat transfer in continuous casting mould is considered. The substantiated equations predict shell growth, temperature distributions, solidification rates and can be used for continuous casters control systems.

  14. FLEXURAL EXPERIMENTAL STUDY ON CONTINUOUS BEAMS STRENGTHENED WITH BFRP%玄武岩纤维布加固混凝土连续梁抗弯试验研究

    Institute of Scientific and Technical Information of China (English)

    欧阳利军; 陆洲导; 丁斌

    2013-01-01

    Continuous basalt fibre reinforced polymer/plastic (BFRP) is a new fibrous composite. Compared with other fiber reinforced polymer/plastic, BFRP has many advantages in ductility, high thermal resistance, corrosion resistance and cost. Flexural experimental are conducted on four two-span T section continuous beams strengthened with BFRP and one comparative beam. Bearing capacity and failure mode of each beam is studied. The results show that yield load, ultimate load, and ductility of strengthened beams are remarkably increased and improved.%玄武岩纤维复合材料(BFRP)是一种新型纤维复合材料,具有价格低、延性好、耐高温和耐腐蚀等优点.为分析玄武岩纤维加固混凝土抗弯构件的受力性能和破坏模式,对4根玄武岩纤维布加固的混凝土T形截面连续梁和1根对比梁进行了抗弯试验.试验表明,玄武岩纤维布加固能显著提高混凝土连续梁的屈服荷载和极限荷裁,加固梁表现出较好的延性.

  15. Comparing the Discrete and Continuous Logistic Models

    Science.gov (United States)

    Gordon, Sheldon P.

    2008-01-01

    The solutions of the discrete logistic growth model based on a difference equation and the continuous logistic growth model based on a differential equation are compared and contrasted. The investigation is conducted using a dynamic interactive spreadsheet. (Contains 5 figures.)

  16. Strategic continuity planning: the first critical step.

    Science.gov (United States)

    Smith, Jack

    2013-01-01

    Many companies (and business continuity professionals) believe a company needs a comprehensive, all-inclusive business continuity plan. Often they reach this conclusion after other companies or potential clients have requested to see their business continuity plan as a precondition of doing business. Companies without 'a plan' are then tempted to go out and hire a business continuity person and tell them to 'Create a plan!' This makes perfect sense to the executive team, but this approach will probably not work in a real event. This paper addresses the shortcomings of producing tactical documentation and calling it 'The Plan', and discusses ways to engage management in the development of a corporate strategy to be used during and after an event.

  17. Bell inequalities with continuous angular variables

    CERN Document Server

    Borges, Carolina V S; Keller, Arne

    2011-01-01

    We consider bipartite quantum systems characterized by a continuous angular variable \\theta \\in [-\\pi, \\pi[, representing, for instance, the position of a particle on a circle. We show how to reveal non-locality on this type of system using inequalities similar to CHSH ones, originally derived for bipartite spin 1/2 like systems. Such inequalities involve correlated measurement of continuous angular functions and are equivalent to the continuous superposition of CHSH inequalities acting on bidimensional subspaces of the infinite dimensional Hilbert space. As an example, we discuss in detail one application of our results, and we derive inequalities based on orientation correlation measurements. The introduced Bell-type inequalities open the perspective of new and simpler experiments to test non locality on a variety of quantum systems described by continuous variables.

  18. Post-postmodernism and Continuing Education.

    Science.gov (United States)

    Leicester, Mal

    2000-01-01

    Attempts to explore the epistemological dimensions of postmodernism. Develops a less problematic post-postmodern perspective, influenced by Wittgenstein. Notes how this perspective is consistent with theory and practice in adult and continuing education. (SK)

  19. Continuous Attributes Discretization Algorithm based on FPGA

    Directory of Open Access Journals (Sweden)

    Guoqiang Sun

    2013-07-01

    Full Text Available The paper addresses the problem of Discretization of continuous attributes in rough set. Discretization of continuous attributes is an important part of rough set theory because most of data that we usually gain are continuous data. In order to improve processing speed of discretization, we propose a FPGA-based discretization algorithm of continuous attributes making use of the speed advantage of FPGA. Combined attributes dependency degree of rough ret, the discretization system was divided into eight modules according to block design. This method can save much time of pretreatment in rough set and improve operation efficiency. Extensive experiments on a certain fighter fault diagnosis validate the effectiveness of the algorithm.  

  20. Continuous Eligibility for Medicaid and CHIP Coverage

    Data.gov (United States)

    U.S. Department of Health & Human Services — States have the option to provide children with 12 months of continuous coverage through Medicaid and CHIP, even if the family experiences a change in income during...

  1. Simple chloride sensors for continuous groundwater monitoring

    DEFF Research Database (Denmark)

    Thorn, Paul; Mortensen, John

    2012-01-01

    The development of chloride sensors which can be used for continuous, on-line monitoring of groundwater could be very valuable in the management of our coastal water resources. However, sensor stability, drift, and durability all need to be addressed in order for the sensors to be used...... in continuous application. This study looks at the development of a simple, inexpensive chloride electrode, and evaluates its performance under continuous use, both in the laboratory and in a field test in a monitoring well. The results from the study showed a consistent response to changing chloride...... sensor remained responsive even at low chloride concentrations, where the conductivity electrode was no longer responding to changing chloride levels. With the results, it is believed that the simple chloride sensor could be used for continuous monitoring of groundwater quality....

  2. Continuous steam hydrolysis of tulip poplar

    Energy Technology Data Exchange (ETDEWEB)

    Fieber, C.A.; Roberts, R.S.; Faass, G.S.; Muzzy, J.D.; Colcord, A.R.; Bery, M.K.

    1982-01-01

    The continuous hydrolysis of poplar chips by steam at 300-350 psi resulted in the separation of hemicellulose (I) cellulose and lignin components. The I fraction was readily depolymerised by steam to acetic acid, furfural, methanol, and xylose.

  3. Continuing Education for Women: Gaining Institutional Credibility

    Science.gov (United States)

    Whatley, Alice Elrod

    1975-01-01

    This article advances an array of hypotheses which are suggested as influential in the establishment and development of programs concerned with continuing education for women on a university campus. The hypotheses are examined and critiqued. (Author/EJT)

  4. Continue morbiditeits registratie peilstations Nederland 1999.

    NARCIS (Netherlands)

    Bartelds, A.I.M.

    2000-01-01

    De Continue Morbiditeitsregisratie Peilstations Nederland is een sinds 1970 bestaand landelijk netwerk van huisartspraktijken. De registratie wordt beheerd door het Nivel. Iedere week melden de deelnemende huisartsen het voorkomen van bepaalde ziektebeelden en gebeurtenissen in hun praktijk. Samen

  5. Detecting categorical perception in continuous discrimination data

    NARCIS (Netherlands)

    Boersma, P.; Chládková, K.

    2010-01-01

    We present a method for assessing categorical perception from continuous discrimination data. Until recently, categorical perception of speech has exclusively been measured by discrimination and identification experiments with a small number of repeatedly presented stimuli. Experiments by Rogers and

  6. Ethical Issues in Marketing and Continuing Education.

    Science.gov (United States)

    Martel, Laurence D.; Colley, Robert M.

    1986-01-01

    Raises ethical considerations relevant to the marketing of continuing education and suggests two approaches to their resolution: deontology (all actions guided by universal rules are moral) and teleology (consequences of an action determine whether it is moral). (CH)

  7. Achieving continuity: a story of stellar magnitude

    Science.gov (United States)

    Evans, Michael S.

    2010-03-01

    Scientists tell a story of 2,000 years of stellar magnitude research that traces back to Hipparchus. This story of continuity in practices serves an important role in scientific education and outreach. STS scholars point out many ways that stories of continuity, like many narratives about science, are disconnected from practices. Yet the story of continuity in stellar magnitude is a powerful scientific achievement precisely because of its connection to practice. The historical development of star catalogues shows how specific recording practices connected past and present in a useful way. The narrative of continuity in stellar magnitude, however else it might be subject to STS critique of narrative, maintains its power because of its connection to practice. I suggest that more attention be paid to connections between practice and narrative in STS, and in particular to the ways that historical practices sustain narratives by connecting past and present.

  8. Alumina Carbon Refractory Products for Continuous Casting

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ 1 Scope This standard specifies the classification.techni-cal requirements,test methods,inspection rules,packing,marking,transportation,storage and quality certificate of alumina carbon refractory products for continuous casting.

  9. Comparing the Discrete and Continuous Logistic Models

    Science.gov (United States)

    Gordon, Sheldon P.

    2008-01-01

    The solutions of the discrete logistic growth model based on a difference equation and the continuous logistic growth model based on a differential equation are compared and contrasted. The investigation is conducted using a dynamic interactive spreadsheet. (Contains 5 figures.)

  10. Continuing Education in Nursing Comes to Mississippi

    Science.gov (United States)

    Rabin, Avis B.

    1971-01-01

    Discusses the growth and development of the Continuing Education in Nursing Department, a part of the School of Nursing at the University of Southern Mississippi. Curriculum development inservice programs, workshops, conferences. (RB)

  11. CERCLA and EPCRA Continuous Release Reporting

    Science.gov (United States)

    Congress established reportable quantities for Superfund hazardous substances. A continuous release of a hazardous substance is defined as being without interruption or abatement and stable in quantity and rate.

  12. Continuous-variable quantum homomorphic signature

    Science.gov (United States)

    Li, Ke; Shang, Tao; Liu, Jian-wei

    2017-10-01

    Quantum cryptography is believed to be unconditionally secure because its security is ensured by physical laws rather than computational complexity. According to spectrum characteristic, quantum information can be classified into two categories, namely discrete variables and continuous variables. Continuous-variable quantum protocols have gained much attention for their ability to transmit more information with lower cost. To verify the identities of different data sources in a quantum network, we propose a continuous-variable quantum homomorphic signature scheme. It is based on continuous-variable entanglement swapping and provides additive and subtractive homomorphism. Security analysis shows the proposed scheme is secure against replay, forgery and repudiation. Even under nonideal conditions, it supports effective verification within a certain verification threshold.

  13. Continuous ethanol fermentation by beer yeast

    Energy Technology Data Exchange (ETDEWEB)

    Kida, Kenji; Morimura, Shigeru; Shima, Noriyuki; Asano, Shinichi; Yamadaki, Motozumi; Miyazawa, Toshiki

    1987-01-25

    Cooked and uncooked continuous fermentation tests and a bench-scale continuous fermentation test were carried out using a flocculating yeast, beer yeast IFO-2018, and using molasses of various origin. Heat of fermentation was measured at the same time. High productivity was found in a non-cooking continuous fermentation, but lowering of a flocculating ability was a problem. A theoretical equation for calculating the heat of fermentation was introduced and its adaptability was examined. The continuous fermentation unit was a single tank type using a tower type fermentor. The fermentor had a capacity of 0.7 l actual volume, made of glass, and consisted of the two parts, a flowing part and a precipitation/separation part. The yeast used was Saccharomyces crevisiae IFO-2018 in comparison with such yeast as S. cerevisiae IFO-0224 and S. cervisiae EP-1. (7 figs,3 tabs,21 refs)

  14. Continuous Interaction within the SAIBA Framework

    NARCIS (Netherlands)

    Zwiers, Jakob; van Welbergen, H.; Reidsma, Dennis; Vilhjálmsson, Hannes Högni; Kopp, Stefan; Marsella, Stacy; Thórisson, Kristinn R.

    We propose extensions to the SAIBA BML language, aiming in particular to specify reactive behavior and continuous interaction. Examples of interaction scenarios are provided that illustrate the usefulness of these extensions. The impact on the SAIBA architecture is discussed.

  15. 38 CFR 3.53 - Continuous cohabitation.

    Science.gov (United States)

    2010-07-01

    ... ADJUDICATION Pension, Compensation, and Dependency and Indemnity Compensation Relationship § 3.53 Continuous... made during the life of the veteran on issues subsequently involved in the application of this section....

  16. Modified Continuous Loop Technique for microvascular anastomosis

    Directory of Open Access Journals (Sweden)

    Kumar Pramod

    2001-01-01

    Full Text Available A modified method of continuous loop technique for microvascular anastomosis is described. The handling of loop is easier & even last suture is placed under vision. This makes the microvascular anastomosis easier and simpler.

  17. Continued Fraction as a Discrete Nonlinear Transform

    CERN Document Server

    Bender, C M; Bender, Carl M.; Milton, Kimball A.

    1993-01-01

    The connection between a Taylor series and a continued-fraction involves a nonlinear relation between the Taylor coefficients $\\{ a_n \\}$ and the continued-fraction coefficients $\\{ b_n \\}$. In many instances it turns out that this nonlinear relation transforms a complicated sequence $\\{a_n \\}$ into a very simple one $\\{ b_n \\}$. We illustrate this simplification in the context of graph combinatorics.

  18. Continuity and invariance in hybrid automata

    OpenAIRE

    Lygeros, John; Johansson, Karl H.; Simic, Slobodan; ZHANG, JUN; Sastry, Shankar

    2001-01-01

    Hybrid automata have been proposed as a language for modelling and analysing the interaction of digital and analogue dynamics in embedded computer systems. In the paper, hybrid automata are studied from a dynamical systems perspective. Extending earlier work on conditions for existence and uniqueness of executions of hybrid automata, we characterise a class of hybrid automata whose executions depend continuously on the initial state. The continuity conditions are subsequently used to derive a...

  19. Alternative forms of continuous assessment in mathematics

    OpenAIRE

    Carr, Michael; Ní Fhloinn, Eabhnat

    2007-01-01

    In Dublin Institute of Technology, there has been a recent move to semesterisation, with an increased emphasis on continuous assessment. In mathematics, this would traditionally mean that students would sit a number of short, written assessments during the course of each semester, in conjunction with an end-of-module exam. However, it was decided to combine these usual assessments with presentations on mathematics for certain cohorts this semester. As part of their continuous assessment ma...

  20. When to carry out analytic continuation?

    CERN Document Server

    Zuo, J M

    1998-01-01

    This paper discusses the analytic continuation in the thermal field theory by using the theory of $\\eta-\\xi$ spacetime. Taking a simple model as example, the $2\\times 2$ matrix real-time propagator is solved from the equation obtained through continuation of the equation for the imaginary-time propagator. The geometry of the $\\eta-\\xi$ spacetime plays important role in the discussion.

  1. Drug Dosing During Continuous Renal Replacement Therapies

    OpenAIRE

    Thompson, A. Jill

    2008-01-01

    Continuous renal replacement therapies (CRRT) are used to manage fluid overload and/or renal failure. The continuous nature of the fluid and solute removal has less impact on hemodynamic variables in critically ill patients, making CRRT preferred over intermittent hemodialysis for some patients in the intensive care arena. The impact of CRRT on drug removal is variable depending on the CRRT modality, the ultrafiltrate and dialysate flow rates, the filter, and the patient's residual renal func...

  2. Method and apparatus for continuous electrophoresis

    Science.gov (United States)

    Watson, Jack S.

    1992-01-01

    A method and apparatus for conducting continuous separation of substances by electrophoresis are disclosed. The process involves electrophoretic separation combined with couette flow in a thin volume defined by opposing surfaces. By alternating the polarity of the applied potential and producing reciprocating short rotations of at least one of the surfaces relative to the other, small increments of separation accumulate to cause substantial, useful segregation of electrophoretically separable components in a continuous flow system.

  3. Process for fabricating continuous lengths of superconductor

    Science.gov (United States)

    Kroeger, Donald M.; List, III, Frederick A.

    1998-01-01

    A process for manufacturing a superconductor. The process is accomplished by depositing a superconductor precursor powder on a continuous length of a first substrate ribbon, overlaying a continuous length of a second substrate ribbon on said first substrate ribbon, and applying sufficient pressure to form a bound layered superconductor precursor between said first substrate ribbon and said second substrates ribbon. The layered superconductor precursor is then heat treated to form a super conductor layer.

  4. Reliability assessment of continuous mining technological systems

    OpenAIRE

    2010-01-01

    Article is devoted to the analysis of reliability of parameters of continuous system of extraction with use of various approaches with the purpose of performance of a fast estimation of general efficiency of the equipment of such industrial systems, as Monte-Carlo and stress-force of intervention. Article is devoted to the analysis of reliability of parameters of continuous system of extraction with use of various approaches with the purpose of performance of a fast estimation of general e...

  5. Continuously-Variable Series-Elastic Actuator

    OpenAIRE

    Mooney, Luke M.; Herr, Hugh M.

    2013-01-01

    Actuator efficiency is an important factor in the design of powered leg prostheses, orthoses, exoskeletons, and legged robots. A continuously-variable series-elastic actuator (CV-SEA) is presented as an efficient actuator for legged locomotion. The CV-SEA implements a continuously-variable transmission (CVT) between a motor and series elastic element. The CVT reduces the torque seen at the motor and allows the motor to operate in speed regimes of higher efficiency, while the series-elastic el...

  6. Continuous first order logic and local stability

    CERN Document Server

    Yaacov, Itaï Ben

    2008-01-01

    We develop continuous first order logic, a variant of the logic described in \\cite{Chang-Keisler:ContinuousModelTheory}. We show that this logic has the same power of expression as the framework of open Hausdorff cats, and as such extends Henson's logic for Banach space structures. We conclude with the development of local stability, for which this logic is particularly well-suited.

  7. Carrier-free, continuous primary beer fermentation

    OpenAIRE

    Pires, Eduardo J.; Teixeira, J. A; Brányik, Tomáš; A.A. Vicente

    2014-01-01

    Developing a sustainable continuous fermentation reactor is one of the most ambitious tasks in brewing science, but it could bring great benefits regarding volumetric productivity to modern breweries. Immobilized cell technology is often applied to reach the large densities of yeast needed in a continuous fermentation process. However, the financial cost associated with the use of carriers for yeast immobilization is one of the major drawbacks in the technology. This work suggests that yeast ...

  8. Creating meaningful business continuity management programme metrics.

    Science.gov (United States)

    Strong, Brian

    2010-11-01

    The popular axiom, 'what gets measured gets done', is often applied in the quality management and continuous improvement disciplines. This truism is also useful to business continuity practitioners as they continually strive to prove the value of their organisation's investment in a business continuity management (BCM) programme. BCM practitioners must also remain relevant to their organisations as executives focus on the bottom line and maintaining stakeholder confidence. It seems that executives always find a way, whether in a hallway or elevator, to ask BCM professionals about the company's level of readiness. When asked, they must be ready with an informed response. The establishment of a process to measure business continuity programme performance and organisational readiness has emerged as a key component of US Department of Homeland Security 'Voluntary Private Sector Preparedness (PS-Prep) Program' standards where the overarching goal is to improve private sector preparedness for disasters and emergencies. The purpose of this paper is two-fold: to introduce continuity professionals to best practices that should be considered when developing a BCM metrics programme as well as providing a case study of how a large health insurance company researched, developed and implemented a process to measure BCM programme performance and company readiness.

  9. Continuous-variable quantum information processing

    DEFF Research Database (Denmark)

    Andersen, Ulrik Lund; Leuchs, G.; Silberhorn, C.

    2010-01-01

    Observables of quantum systems can possess either a discrete or a continuous spectrum. For example, upon measurements of the photon number of a light state, discrete outcomes will result whereas measurements of the light's quadrature amplitudes result in continuous outcomes. If one uses the conti......Observables of quantum systems can possess either a discrete or a continuous spectrum. For example, upon measurements of the photon number of a light state, discrete outcomes will result whereas measurements of the light's quadrature amplitudes result in continuous outcomes. If one uses...... the continuous degree of freedom of a quantum system for encoding, processing or detecting information, one enters the field of continuous-variable (CV) quantum information processing. In this paper we review the basic principles of CV quantum information processing with main focus on recent developments...... in the field. We will be addressing the three main stages of a quantum information system; the preparation stage where quantum information is encoded into CVs of coherent states and single-photon states, the processing stage where CV information is manipulated to carry out a specified protocol and a detection...

  10. Composição químico-bromatológica do feno de cipó-de-escada (Bauhinia glabra Jacq. em cinco estádios de corte Chemical composition of the hay of the Bauhinia glabra Jacq. in five periods of cut

    Directory of Open Access Journals (Sweden)

    Jael Soares Batista

    1999-01-01

    Full Text Available Esta pesquisa foi realizada para avaliar a composição químico-bromatológica do feno de cipó-de-escada (Bauhinia glabra Jacq. aos 42, 56, 70, 84 e 98 dias de crescimento, após o corte de uniformização. Delineamento experimental inteiramente casualizado com cinco tratamentos e quatro repetições foi usado. O feno foi obtido em uma área de pastagem nativa rebaixada/raleada de 7200 m², subdividida em parcelas de 360 m². Não houve diferenças para os teores de matéria seca (MS, matéria orgânica (MO, proteína bruta (PB, hemicelulose e fósforo (P. Os teores de fibra em detergente neutro (FDN, fibra em detergente ácido (FDA, celulose, lignina e cálcio (Ca apresentaram diferenças entre os tratamentos. Os teores médios para a composição químico-bromatológica foram: 88,90% MS, 94,17% MO, 5,51% MM, 17,97% PB, 58,12% FDN, 40,88% FDA, 17,11% hemicelulose, 27,33% celulose, 12,77% lignina, 0,29% Ca e 0,15% P. Os fenos avaliados são recomendáveis para suplementação protéica em dietas para ruminantes. Com o avançar do estádio vegetativo, aumentaram linearmente os constituintes da parede celular e o Ca.This research was carried out to evaluate the chemical composition of cipó-de-escada (Bauhinia glabra Jacq hay at 42, 56, 70, 84 and 98 days of growth after uniformity cut. A completely randomized experimental design, with five treatments and four replicates, was used. The hay was obtained from an area of native pasture lowed and cleaned of 7200 m², which were subdivided in plots of 360 m². There were no differences for the content of dry matter (DM, organic matter (OM, crude protein (CP, hemicellulose and phosphorus (P. However, differences were observed for the neutral detergent fiber (NDF, acid detergent fiber (ADF, cellulose, lignin and calcium (Ca contents among the treatments. The average contents for the chemical composition were: 88.90% DM, 94.17% OM, 5.51% ash, 17.97% CP, 58.12% NDF, 40.88% ADF, 17.11% hemicellulose, 27

  11. Tests and FEA of continuous concrete-partial-filled steel box beams%部分充填混凝土钢箱连续梁试验及有限元分析

    Institute of Scientific and Technical Information of China (English)

    陈伟; 莫时旭; 陈永健; 陈丕钰; 卫士杰

    2015-01-01

    通过2个两跨部分充填混凝土钢箱连续梁和1个钢箱连续梁模型在两个集中力作用下的试验,研究其变形发展及破坏过程。试验结果表明,部分充填混凝土钢箱连续梁模型与钢箱连续梁模型相比,弹性承载能力提高45%,极限承载力提高90%,延性增大8倍。利用ANSYS对部分充填混凝土钢箱连续梁受力行为进行了非线性有限元分析,分析结果与试验实测结果吻合良好,验证了计算模型的有效性,利用有限元模型参数分析表明增大受拉区翼板厚度对承载力效果最优。%In the tests of 2 two-span continuous concrete partially filled steel box beams (CCPFSBB) and a two-span continuous steel box beam (CSBB) subjected two forces, the deformation and failure pattern of the beams were studied.The results showed that the elastic loading capacity and ultimate loading capacity of CCPFSBB model were 45%and 90%larger than those of CSBB model respectively, and the ductility also increased about 8 times.Non-linear FEA of the mechanical behavior of the CCPFSBB was conducted with ANSYS.The an-alytic results agreed with the test results, and the analytic FEM model was verified.The par-ametric analysis was carried out using the FEA model.The results showed that the optimal effect on improving the capacity was increasing the flange plate thickness.

  12. Momilactone B induces apoptosis and G1 arrest of the cell cycle in human monocytic leukemia U937 cells through downregulation of pRB phosphorylation and induction of the cyclin-dependent kinase inhibitor p21Waf1/Cip1.

    Science.gov (United States)

    Park, Cheol; Jeong, Na Young; Kim, Gi-Young; Han, Min Ho; Chung, Ill-Min; Kim, Wun-Jae; Yoo, Young Hyun; Choi, Yung Hyun

    2014-04-01

    Momilactone B, a terpenoid phytoalexin present in rice bran, has been shown to exhibit several biological activities. The present study was conducted using cultured human leukemia U937 cells to elucidate the possible mechanisms by which momilactone B exerts its anticancer activity, which to date has remained poorly understood. Momilactone B treatment of U937 cells resulted in a dose-dependent inhibition of cell growth and induced apoptotic cell death as detected by chromatin condensation, DNA fragmentation, the cleavage of poly(ADP-ribose) polymerase and Annexin V-FITC staining. Flow cytometric analysis revealed that momilactone B resulted in G1 arrest in cell cycle progression, which was associated with the dephosphorylation of retinoblastoma protein (pRB) and enhanced binding of pRB with the E2F transcription factor family proteins. Treatment with momilactone B also increased the expression of cyclin-dependent kinase (Cdk) inhibitor p21Waf1/Cip1 in a p53-independent manner, without any noticeable changes in G1 cyclins and cyclin-dependent kinases (Cdks), except a slight decrease in cyclin E. Moreover, in vitro kinase assay indicated that momilactone B significantly decreased Cdk4- and Cdk6-associated kinase activities through a notably increased binding of p21 to Cdk4 and Cdk6. Our results demonstrated that momilactone B caused G1 cell cycle arrest and apoptosis in U937 cells through the induction of p21 expression, inhibition of Cdk/cyclin-associated kinase activities, and reduced phosphorylation of pRB, which may be related to anticancer activity.

  13. Design Feature 7: Continuous Preclosure Ventilation

    Energy Technology Data Exchange (ETDEWEB)

    A.T. Watkins

    1999-06-22

    This design feature (DF) is intended to evaluate the effects of continuous ventilation in the emplacement drifts during preclosure and how the effects, if any, compare to the Viability Assessment (VA) reference design for postclosure long term performance. This DF will be evaluated against a set of criteria provided by the License Application Design Selection (LADS) group. The VA reference design included a continuous ventilation airflow quantity of 0.1 m{sup 3}/s in the emplacement drifts in the design of the repository subsurface facilities. The effects of this continuous ventilation during the preclosure was considered to have a negligible effect on postclosure performance and therefore is not included during postclosure in the assessment of the long term performance. This DF discusses the effects of continuous ventilation on the emplacement drift environment and surrounding rock conditions during preclosure for three increased airflow quantities. The three cases of continuous ventilation systems are: System A, 1.0 m{sup 3}/s (Section 8), System B, 5.0 m{sup 3}/s (Section 9), and System C, 10.0 m{sup 3}/s (Section 10) in each emplacement drift split. An emplacement drift split is half total length of emplacement drift going from the east or west main to the exhaust main. The difference in each system is the quantity of airflow in the emplacement drifts.

  14. Manufacturing laser glass by continuous melting

    Energy Technology Data Exchange (ETDEWEB)

    Campbell, J H; Suratwala, T; krenitsky, S; Takeuchi, K

    2000-07-01

    A novel, continuous melting process is being used to manufacture meter-sized plates of laser glass at a rate 20-times faster, 5-times cheaper, and with 2-3 times better optical quality than with previous one-at-a-time, ''discontinuous'' technology processes. This new technology for manufacturing laser glass, which is arguably the most difficult continuously-melted optical material ever produced, comes as a result of a $60 million, six-year joint R&D program between government and industry. The glasses manufactured by the new continuous melting process are Nd-doped phosphate-based glasses and are marketed under the product names LG-770 (Schott Glass Technologies) and LHG-8 (Hoya Corporation USA). With this advance in glass manufacturing technology, it is now possible to construct high-energy, high-peak-power lasers for use in fusion energy development, national defense, and basic physics research that would have been impractical to build using the old melting technology. The development of continuously melted laser glass required technological advances that have lead to improvements in the manufacture of other optical glass products as well. For example, advances in forming, annealing, and conditioning steps of the laser glass continuous melting process are now being used in manufacture of other large-size optical glasses.

  15. Continuity of care in community midwifery.

    Science.gov (United States)

    Bowers, John; Cheyne, Helen; Mould, Gillian; Page, Miranda

    2015-06-01

    Continuity of care is often critical in delivering high quality health care. However, it is difficult to achieve in community health care where shift patterns and a need to minimise travelling time can reduce the scope for allocating staff to patients. Community midwifery is one example of such a challenge in the National Health Service where postnatal care typically involves a series of home visits. Ideally mothers would receive all of their antenatal and postnatal care from the same midwife. Minimising the number of staff-handovers helps ensure a better relationship between mothers and midwives, and provides more opportunity for staff to identify emerging problems over a series of home visits. This study examines the allocation and routing of midwives in the community using a variant of a multiple travelling salesmen problem algorithm incorporating staff preferences to explore trade-offs between travel time and continuity of care. This algorithm was integrated in a simulation to assess the additional effect of staff availability due to shift patterns and part-time working. The results indicate that continuity of care can be achieved with relatively small increases in travel time. However, shift patterns are problematic: perfect continuity of care is impractical but if there is a degree of flexibility in the visit schedule, reasonable continuity is feasible.

  16. Measuring continuity of care: psychometric properties of the Nijmegen Continuity Questionnaire

    Science.gov (United States)

    Uijen, Annemarie A; Schers, Henk J; Schellevis, François G; Mokkink, Henk GA; van Weel, Chris; van den Bosch, Wil JHM

    2012-01-01

    Background Recently, the Nijmegen Continuity Questionnaire (NCQ) was developed. It aims to measure continuity of care from the patient perspective across primary and secondary care settings. Initial pilot testing proved promising. Aim To further examine the validity, discriminative ability, and reliability of the NCQ. Design A prospective psychometric instrument validation study in primary and secondary care in the Netherlands. Method The NCQ was administered to patients with a chronic disease recruited from general practice (n = 145) and hospital outpatient departments (n = 123) (response rate 76%). A principal component analysis was performed to confirm three subscales that had been found previously. Construct validity was tested by correlating the NCQ score to scores of other scales measuring quality of care, continuity, trust, and satisfaction. Discriminative ability was tested by investigating differences in continuity subscores of different subgroups. Test–retest reliability was analysed in 172 patients. Results Principal factor analysis confirmed the previously found three continuity subscales — personal continuity, care provider knows me; personal continuity, care provider shows commitment; and team/cross-boundary continuity. Construct validity was demonstrated through expected correlations with other variables and discriminative ability through expected differences in continuity subscores of different subgroups. Test–retest reliability was high (the intraclass correlation coefficient varied between 0.71 and 0.82). Conclusion This study provides evidence for the validity, discriminative ability, and reliability of the NCQ. The NCQ can be of value to identify problems in continuity of care. PMID:22782001

  17. Continuous recovery system for electrorefiner system

    Science.gov (United States)

    Williamson, Mark A.; Wiedmeyer, Stanley G.; Willit, James L.; Barnes, Laurel A.; Blaskovitz, Robert J.

    2014-06-10

    A continuous recovery system for an electrorefiner system may include a trough having a ridge portion and a furrow portion. The furrow portion may include a first section and a second section. An inlet and exit pipe may be connected to the trough. The inlet pipe may include an outlet opening that opens up to the first section of the furrow portion of the trough. The exit pipe may include an entrance opening that opens up to the second section of the furrow portion of the trough. A chain may extend through the inlet and exit pipes and along the furrow portion of the trough. The chain may be in a continuous loop form. A plurality of flights may be secured to the chain. Accordingly, the desired product may be continuously harvested from the electrorefiner system without having to halt the electrical power and/or remove the cathode and anode assemblies.

  18. Discrete and continuous simulation theory and practice

    CERN Document Server

    Bandyopadhyay, Susmita

    2014-01-01

    When it comes to discovering glitches inherent in complex systems-be it a railway or banking, chemical production, medical, manufacturing, or inventory control system-developing a simulation of a system can identify problems with less time, effort, and disruption than it would take to employ the original. Advantageous to both academic and industrial practitioners, Discrete and Continuous Simulation: Theory and Practice offers a detailed view of simulation that is useful in several fields of study.This text concentrates on the simulation of complex systems, covering the basics in detail and exploring the diverse aspects, including continuous event simulation and optimization with simulation. It explores the connections between discrete and continuous simulation, and applies a specific focus to simulation in the supply chain and manufacturing field. It discusses the Monte Carlo simulation, which is the basic and traditional form of simulation. It addresses future trends and technologies for simulation, with par...

  19. Lifted Inference for Relational Continuous Models

    CERN Document Server

    Choi, Jaesik; Hill, David J

    2012-01-01

    Relational Continuous Models (RCMs) represent joint probability densities over attributes of objects, when the attributes have continuous domains. With relational representations, they can model joint probability distributions over large numbers of variables compactly in a natural way. This paper presents a new exact lifted inference algorithm for RCMs, thus it scales up to large models of real world applications. The algorithm applies to Relational Pairwise Models which are (relational) products of potentials of arity 2. Our algorithm is unique in two ways. First, it substantially improves the efficiency of lifted inference with variables of continuous domains. When a relational model has Gaussian potentials, it takes only linear-time compared to cubic time of previous methods. Second, it is the first exact inference algorithm which handles RCMs in a lifted way. The algorithm is illustrated over an example from econometrics. Experimental results show that our algorithm outperforms both a groundlevel inferenc...

  20. Formalising the Continuous/Discrete Modeling Step

    Directory of Open Access Journals (Sweden)

    Wen Su

    2011-06-01

    Full Text Available Formally capturing the transition from a continuous model to a discrete model is investigated using model based refinement techniques. A very simple model for stopping (eg. of a train is developed in both the continuous and discrete domains. The difference between the two is quantified using generic results from ODE theory, and these estimates can be compared with the exact solutions. Such results do not fit well into a conventional model based refinement framework; however they can be accommodated into a model based retrenchment. The retrenchment is described, and the way it can interface to refinement development on both the continuous and discrete sides is outlined. The approach is compared to what can be achieved using hybrid systems techniques.

  1. Formalising the Continuous/Discrete Modeling Step

    CERN Document Server

    Banach, Richard; Su, Wen; Huang, Runlei; 10.4204/EPTCS.55.8

    2011-01-01

    Formally capturing the transition from a continuous model to a discrete model is investigated using model based refinement techniques. A very simple model for stopping (eg. of a train) is developed in both the continuous and discrete domains. The difference between the two is quantified using generic results from ODE theory, and these estimates can be compared with the exact solutions. Such results do not fit well into a conventional model based refinement framework; however they can be accommodated into a model based retrenchment. The retrenchment is described, and the way it can interface to refinement development on both the continuous and discrete sides is outlined. The approach is compared to what can be achieved using hybrid systems techniques.

  2. CONTINUOUS IMPROVEMENT THROUGH INTEGRATION OF QUALITY TOOLS

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    The relationship between major quality tools such as quality function development (QFD),failure mode and effects analysis (FMEA), design of experiments (DOE) and statistical process control (SPC) is analyzed through an extensive review of the literature and the concurrent quality engineering philosophy, and a basic structure for the integration of quality tools is presented. An integrated quality management system (IQMS) is developed using C++ Builder, nmning in the Windows 2000 Server environment with the basic internet connections, and SQL Server 2000 as the platform for developing the database. An illustrative example applying IQMS to the continuous quality improvement for a crane equipment manufacturing is reported. The result shows that the application of IQMS can optimize the process of design and manufacturing, shorten the cycle time of product, reduce the cost, and realize quality improvement continuously. The proposed integrated framework with IQMS is believed to be applicable to continuous quality improvement in many manufacturing companies.

  3. Continuation of Sets of Constrained Orbit Segments

    DEFF Research Database (Denmark)

    Schilder, Frank; Brøns, Morten; Chamoun, George Chaouki

    Sets of constrained orbit segments of time continuous flows are collections of trajectories that represent a whole or parts of an invariant set. A non-trivial but simple example is a homoclinic orbit. A typical representation of this set consists of an equilibrium point of the flow and a trajecto...... orbit segments, this is very disadvantageous in practice. In this talk we will present an algorithm that allows the efficient continuation of sets of constrained orbit segments together with the solution of the full variational problem.......Sets of constrained orbit segments of time continuous flows are collections of trajectories that represent a whole or parts of an invariant set. A non-trivial but simple example is a homoclinic orbit. A typical representation of this set consists of an equilibrium point of the flow and a trajectory...

  4. Continuous retrieval of delayed Raman polaritons

    CERN Document Server

    Smartsev, Slava; Davidson, Nir; Firstenberg, Ofer

    2016-01-01

    We use a Raman four-wave mixing process to read out light from an atomic coherence which is continuously written. This realizes a continuous source of polaritons having been delayed or effectively stored for a finite duration. Contrary to slow-light delay, which depends on the atom number and population distribution, here the effective storage duration is determined solely by intensive properties of the system, approaching the ground-state natural lifetime at the weak driving limit. The generated polaritons are background free. We experimentally probe these properties utilizing spatial atomic diffusion as an 'internal clock' for the write-read delay. A continuous source of delayed polaritons can replace discrete write-read procedures when the atomic evolution is the subject of interest, for example, when dipolar interactions lead to retrieval of non-classical light.

  5. FORMULAS OF TENSION OF CONTINUOUS ROLLING PROCESS

    Institute of Scientific and Technical Information of China (English)

    J.Z. Zhang; X.P. Zhang

    2007-01-01

    The development of computer controlled continuous rolling process calls for a mathematicalexpression that can express the inequality condition of "constant flow". Tension is the link of thecontinuous rolling process. From the condition of dynamic equilibrium, a differential equation oftension is given out. On the basis of the physical rules established from the industrial practice andexperimental studies, the law of volume constancy, the linear relation of forward slip and tension,the state equation of continuous rolling, the formula of dynamic tension, and the formula of statictension have been obtained. These formulae reflect the functional relations between tensions,thickness, roll velocity, and time in the continuous rolling process. It is implied that the continuousrolling process is a gradually steady, controllable, and measurable dynamic system. An assumptionof predicting the thickness of a steel plate using these tension formulae is also put forward.

  6. Mathematical Model for the Continuous Vacuum Drying

    Institute of Scientific and Technical Information of China (English)

    DAI Hui-liang

    2002-01-01

    An improved mathematical model for the continuous vacuum drying of highly viscous and heatsensitive foodstuffs was proposed, The process of continuous vacuum drying was presented as a moving boundary problem of moisture evaporation in cylindrical coordinates. Boundary condition of the first kind for the known functional dependence of the drying body surface temperature on time was considered. Finally, the appropriate system of differential equations was solved numerically and the values of drying rate, integral moisture content of the material, moving boundary position as well as temperature in any point of the material and at any moment time were obtained. This procedure was applied to continuous vacuum drying of foods such as natural cheese and fresh meat paste.

  7. National Security Technology Incubation Project Continuation Plan

    Energy Technology Data Exchange (ETDEWEB)

    None

    2008-09-30

    This document contains a project continuation plan for the National Security Technology Incubator (NSTI). The plan was developed as part of the National Security Preparedness Project (NSPP) funded by a Department of Energy (DOE)/National Nuclear Security Administration (NNSA) grant. This continuation plan describes the current status of NSTI (staffing and clients), long-term goals, strategies, and long-term financial solvency goals.The Arrowhead Center of New Mexico State University (NMSU) is the operator and manager of the NSTI. To realize the NSTI, Arrowhead Center must meet several performance objectives related to planning, development, execution, evaluation, and sustainability. This continuation plan is critical to the success of NSTI in its mission of incubating businesses with security technology products and services.

  8. Confining continuous manipulations of accelerator beamline optics

    CERN Document Server

    Amstutz, Philipp; Bödewadt, Jörn; Lechner, Christoph; Plath, Tim; Vogt, Mathias

    2016-01-01

    Altering the optics in one section of a linear accelerator beamline will in general cause an alteration of the optics in all downstream sections. In circular accelerators, changing the optical properties of any beamline element will have an impact on the optical functions throughout the whole machine. In many cases, however, it is desirable to change the optics in a certain beamline section without disturbing any other parts of the machine. Such a local optics manipulation can be achieved by adjusting a number of additional corrector magnets that restore the initial optics after the manipulated section. In that case, the effect of the manipulation is confined in the region between the manipulated and the correcting beamline elements. Introducing a manipulation continuously, while the machine is operating, therefore requires continuous correction functions to be applied to the correcting quadrupole magnets. In this paper we present an analytic approach to calculate such continuous correction functions for six ...

  9. Continuity Adjustment for Control Charts for Attributes

    Institute of Scientific and Technical Information of China (English)

    L.K. Chan; T.K. Mak; B. Tao

    2003-01-01

    A unified approach is proposed for making a continuity adjustment on some control charts for attributes, e.g., np-chart and c-chart, through adding a uniform (0, 1) random observation to the conventional sample statistic (e.g., npi and ci). The adjusted sample statistic then has a continuous distribution. Consequently, given any Type I risk α (the probability that the sample statistic is on or beyond the control limits),control charts achieving the exact value of α can be readily constructed. Guidelines are given for when to use the continuity adjustment control chart, the conventional Shewhart control chart (with ±3 standard deviations control limits), and the control chart based on the exact distribution of the sample statistic before adjustment.

  10. Continuous Observations and the Wave Function Collapse

    CERN Document Server

    Marchewka, A

    2011-01-01

    We propose to modify the collapse axiom of quantum measurement theory by replacing the instantaneous with a continuous collapse of the wave function in finite time $\\tau$. We apply it to coordinate measurement of a free quantum particle that is initially confined to a domain $D\\subset\\rR^d$ and is observed continuously by illuminating $\\rR^d-D$. The continuous collapse axiom (CCA) defines the post-measurement wave function (PMWF)in $D$ after a negative measurement as the solution of Schr\\"odinger's equation at time $\\tau$ with instantaneously collapsed initial condition and homogeneous Dirichlet condition on the boundary of $D$. The CCA applies to all cases that exhibit the Zeno effect. It rids quantum mechanics of the unphysical artifacts caused by instantaneous collapse and introduces no new artifacts.

  11. Experiments with Externally Prestressed Continuous Composite Girders

    Directory of Open Access Journals (Sweden)

    M. Safan

    2001-01-01

    Full Text Available Steel-concrete composite girders have attractive potentials when applied in bridge construction. The serviceability performance of continuous composite girders is becoming more and more a deterministic parameter in the design of this type of structures. An effective method for improving this performance is to apply prestressing to control or completely eliminate concrete deck cracking caused by static and time dependent actions. Little literature has been found addressing the experimental analysis of continuous girders prestressed by means of external deviated tendons. The current research aims to investigate the behavior of a double-span steel composite beam externally prestressed by means of continuous tendons in terms of cracking characteristics, load deflection response, and load carrying capacity. The efficiency of prestressing is evaluated by comparing the results to those of a non-prestressed beam with similar cross sections and spans.

  12. Mastering the Journey to Continuous Compliance

    Directory of Open Access Journals (Sweden)

    Kimberly Hanna

    2016-06-01

    Full Text Available The accreditation process of a nursing program requires self-assessment, peer evaluation, and identifying areas of improvement. In 2008, the Commission on Collegiate Nursing Education (CCNE began offering a 10-year accreditation with a Continuous Improvement Progress Report (CIPR at the fifth year. This article focuses on an in-depth analysis of a system in which the school of nursing utilized an ad hoc committee to complete the CIPR. Reports generated by the ad hoc committee concluded that need for improvement was warranted related to policy review. An action plan for continuous compliance generated implementation of policy software and the creation of an academic support specialist position. An ad hoc committee completed the CIPR rather than paying faculty overload hours; policy was an exemplar. Faculty development, team performance, and accountability resulted in a plan for continual compliance that can be adopted in other nursing schools to meet CCNE accreditation.

  13. Applications of a fast, continuous wavelet transform

    Energy Technology Data Exchange (ETDEWEB)

    Dress, W.B.

    1997-02-01

    A fast, continuous, wavelet transform, based on Shannon`s sampling theorem in frequency space, has been developed for use with continuous mother wavelets and sampled data sets. The method differs from the usual discrete-wavelet approach and the continuous-wavelet transform in that, here, the wavelet is sampled in the frequency domain. Since Shannon`s sampling theorem lets us view the Fourier transform of the data set as a continuous function in frequency space, the continuous nature of the functions is kept up to the point of sampling the scale-translation lattice, so the scale-translation grid used to represent the wavelet transform is independent of the time- domain sampling of the signal under analysis. Computational cost and nonorthogonality aside, the inherent flexibility and shift invariance of the frequency-space wavelets has advantages. The method has been applied to forensic audio reconstruction speaker recognition/identification, and the detection of micromotions of heavy vehicles associated with ballistocardiac impulses originating from occupants` heart beats. Audio reconstruction is aided by selection of desired regions in the 2-D representation of the magnitude of the transformed signal. The inverse transform is applied to ridges and selected regions to reconstruct areas of interest, unencumbered by noise interference lying outside these regions. To separate micromotions imparted to a mass-spring system (e.g., a vehicle) by an occupants beating heart from gross mechanical motions due to wind and traffic vibrations, a continuous frequency-space wavelet, modeled on the frequency content of a canonical ballistocardiogram, was used to analyze time series taken from geophone measurements of vehicle micromotions. By using a family of mother wavelets, such as a set of Gaussian derivatives of various orders, features such as the glottal closing rate and word and phrase segmentation may be extracted from voice data.

  14. Multimedia Mapping using Continuous State Space Models

    DEFF Research Database (Denmark)

    Lehn-Schiøler, Tue

    2004-01-01

    In this paper a system that transforms speech waveforms to animated faces are proposed. The system relies on continuous state space models to perform the mapping, this makes it possible to ensure video with no sudden jumps and allows continuous control of the parameters in 'face space'. Simulations...... are performed on recordings of 3-5 sec. video sequences with sentences from the Timit database. The model is able to construct an image sequence from an unknown noisy speech sequence fairly well even though the number of training examples are limited....

  15. Continuous-Discrete Path Integral Filtering

    Directory of Open Access Journals (Sweden)

    Bhashyam Balaji

    2009-08-01

    Full Text Available A summary of the relationship between the Langevin equation, Fokker-Planck-Kolmogorov forward equation (FPKfe and the Feynman path integral descriptions of stochastic processes relevant for the solution of the continuous-discrete filtering problem is provided in this paper. The practical utility of the path integral formula is demonstrated via some nontrivial examples. Specifically, it is shown that the simplest approximation of the path integral formula for the fundamental solution of the FPKfe can be applied to solve nonlinear continuous-discrete filtering problems quite accurately. The Dirac-Feynman path integral filtering algorithm is quite simple, and is suitable for real-time implementation.

  16. Configurational space continuity and free energy calculations

    CERN Document Server

    Tian, Pu

    2016-01-01

    Free energy is arguably the most importance function(al) for understanding of molecular systems. A number of rigorous and approximate free energy calculation/estimation methods have been developed over many decades. One important issue, the continuity of an interested macrostate (or path) in configurational space, has not been well articulated, however. As a matter of fact, some important special cases have been intensively discussed. In this perspective, I discuss the relevance of configurational space continuity in development of more efficient and reliable next generation free energy methodologies.

  17. Limitations of Continuous Glucose Monitor Usage.

    Science.gov (United States)

    Anhalt, Henry

    2016-03-01

    Much progress has been made in diabetes treatments since the first dose of insulin was administered in 1921. However, a truly transformational moment in diabetes care occurred when urine testing gave way to capillary blood home glucose monitoring. As improvements were made to these devices, continuous glucose monitoring (CGM) was introduced. The advantages of experiential learnings gleaned from seeing continuous real-time data have been borne out in numerous peer-reviewed journals. Limitations to use of CGM include patient's level of numeracy and literacy, development of alarm fatigue, interfering substances leading to erroneous readings, high rates of discontinuation, and poor reimbursement.

  18. Hybrid Discrete-Continuous Markov Decision Processes

    Science.gov (United States)

    Feng, Zhengzhu; Dearden, Richard; Meuleau, Nicholas; Washington, Rich

    2003-01-01

    This paper proposes a Markov decision process (MDP) model that features both discrete and continuous state variables. We extend previous work by Boyan and Littman on the mono-dimensional time-dependent MDP to multiple dimensions. We present the principle of lazy discretization, and piecewise constant and linear approximations of the model. Having to deal with several continuous dimensions raises several new problems that require new solutions. In the (piecewise) linear case, we use techniques from partially- observable MDPs (POMDPS) to represent value functions as sets of linear functions attached to different partitions of the state space.

  19. Parameter Estimation in Continuous Time Domain

    Directory of Open Access Journals (Sweden)

    Gabriela M. ATANASIU

    2016-12-01

    Full Text Available This paper will aim to presents the applications of a continuous-time parameter estimation method for estimating structural parameters of a real bridge structure. For the purpose of illustrating this method two case studies of a bridge pile located in a highly seismic risk area are considered, for which the structural parameters for the mass, damping and stiffness are estimated. The estimation process is followed by the validation of the analytical results and comparison with them to the measurement data. Further benefits and applications for the continuous-time parameter estimation method in civil engineering are presented in the final part of this paper.

  20. Continuous flow nitration in miniaturized devices.

    Science.gov (United States)

    Kulkarni, Amol A

    2014-01-01

    This review highlights the state of the art in the field of continuous flow nitration with miniaturized devices. Although nitration has been one of the oldest and most important unit reactions, the advent of miniaturized devices has paved the way for new opportunities to reconsider the conventional approach for exothermic and selectivity sensitive nitration reactions. Four different approaches to flow nitration with microreactors are presented herein and discussed in view of their advantages, limitations and applicability of the information towards scale-up. Selected recent patents that disclose scale-up methodologies for continuous flow nitration are also briefly reviewed.