A synthetic NO reduction cycle on a bis(pyrazolato)-bridged dinuclear ruthenium complex including photo-induced transformation.

Science.gov (United States)

Arikawa, Yasuhiro; Hiura, Junko; Tsuchii, Chika; Kodama, Mika; Matsumoto, Naoki; Umakoshi, Keisuke

2018-05-17

A synthetic NO reduction cycle (2NO + 2H+ + 2e- → N2O + H2O) on a dinuclear platform {(TpRu)2(μ-pz)2} (Tp = HB(pyrazol-1-yl)3) was achieved, where an unusual N-N coupling complex was included. Moreover, an interesting photo-induced conversion of the N-N coupling complex to an oxido-bridged complex was revealed.

TP53 gene status affects survival in advanced mycosis fungoides

Directory of Open Access Journals (Sweden)

Gitte Wooler

2016-11-01

Full Text Available TP53 is frequently mutated in different types of neoplasms including leukemia and lymphomas. Mutations of TP53 have also been reported in mycosis fungoides (MF, the most common type of cutaneous lymphoma. However, little is known about the frequency, spectrum of mutations and their prognostic significance in MF. In this study we have optimized the protocol for Sanger sequencing of TP53 using DNA extracted from archival paraffin-embedded biopsies. Of 19 samples from patients with stage IIB MF or higher, 31% harboured mutations in TP53. Overall survival of the patients with mutated TP53 was significantly shorter than median survival in the age- and stage-matched patients treated in our Institution. Distribution of mutations was heterogenous in TP53 exons, however C>T transitions were common suggesting the causal role of ultraviolet radiation. We propose that TP53 mutation status would be useful for risk stratification of patients with advanced MF.

On the simultaneous Pell equations x 2 - (4m 2 - 1)y 2 = y 2 - pz 2 ...

African Journals Online (AJOL)

Let m be a positive integer, and let p be an odd prime. By using certain properties of Pell and quartic diophantine equations with some elementary number theory methods, we prove that the system of equations x2 - (4m2 - 1)y2 = 1 and y2 - pz2 = 1 has positive integer solutions (x, y, z) if and only if p ≡ 7(mod 8) and m = 1/4 ...

Prolonged Tp-e Interval in Down Syndrome Patients with Congenitally Normal Hearts.

Science.gov (United States)

Kucuk, Mehmet; Karadeniz, Cem; Ozdemir, Rahmi; Meşe, Timur

2018-03-25

Heterogeneity of ventricular repolarization has been assessed by using the QT dispersion in Down syndrome (DS) patients with congenitally normal hearts. However, novel repolarization indexes, the Tp-e interval and Tp-e/QT ratio, have not previously been evaluated in these patients. The aim of this study was to evaluate the Tp-e interval and Tp-e/QT ratio in DS patients without congenital heart defects. Twelve-lead surface electrocardiograms of 160 DS patients and 110 age- and sex-matched healthy controls were used to evaluate and compare the Tp-e interval, Tp-e dispersion, and Tp-e/QT ratio. Heart rate, Tp-e interval, Tp-e dispersion, Tp-e/QT and Tp-e/QTc ratios were significantly higher in DS group than in the controls. Myocardial repolarization indexes in DS patients with congenitally normal hearts were found to be prolonged compared to those in normal controls. Further evaluation is warranted to reveal a relationship between prolonged repolarization indexes and arrhythmic events in these patients. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

Main - TP Atlas | LSDB Archive [Life Science Database Archive metadata

Lifescience Database Archive (English)

Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data ...me: tp_atlas_en.zip File URL: ftp://ftp.biosciencedbc.jp/archive/tp_atlas/LATEST/...d License Update History of This Database Site Policy | Contact Us Main - TP Atlas | LSDB Archive ...

Biotransformation of hydralazine (HDZ) in monolayer cultures of rabbit hepatocytes

International Nuclear Information System (INIS)

McQueen, C.A.; Rosado, R.R.

1990-01-01

Adverse reactions to HDZ have been associated with the acetylator polymorphism; slow acetylators are more likely to develop HDZ-induced lupus erythematosus. In studying the role of this polymorphism in susceptibility to HDZ toxicity, the biotransformation of HDZ was investigated in rabbit hepatocytes. New Zealand white rabbits, like humans, are classified as rapid or slow acetylators. Heptocytes were isolated from rapid acetylator rabbits by collagenase perfusion. Monolayer cultures were initiated and exposed to 14 C-HDZ. Since HDZ is unstable at neutral pH, parallel incubations were done in the absence of cells. Metabolites in the media were determined by reverse phase HPLC. Phthalazine (P), phthalazinone (PZ), triazoloph-thalazine (TP), methyl TP (MTP) and 3-hydroxy MTP were identified. In the absence of cells, more TP was formed than MTP, probably resulting from reaction of HDZ with components in the medium. In the presence of cells, there was a three-fold increase in MTP, while the amount of TP was relatively constant. Only trace amounts of P, PZ 3-hydroxy MTP were detected. These data indicate that monolayer cultures of rapid acetylator rabbit hepatocytes were capable of metabolizing HDZ with acetylation playing a major role. These studies are being extended to cells from slow acetylator rabbits

TP Organics - maheuuringute tehnoloogiaplatvorm

Index Scriptorium Estoniae

2015-01-01

TP Organics on 2008. a loodud Euroopa mahepõllumajanduse ja mahetoidu ning vähese sisendiga põllumajanduse tehnoloogiaplatvorm. Platvormi eesmärk on selgitada välja teadustöö ja uuenduste vajadused ning edastada see info poliitikutele

Analytical and clinical comparison of Elecsys syphilis (Roche®) - Architect syphilis TP and reformulated Architect syphilis TP (Abbott®) assay.

Science.gov (United States)

De Keukeleire, Steven; Desmet, Stefanie; Lagrou, Katrien; Oosterlynck, Julie; Verhulst, Manon; Van Besien, Jessica; Saegeman, Veroniek; Reynders, Marijke

2017-03-01

The performance of Elecsys Syphilis was compared to Architect Syphilis TP and Reformulated Architect Syphilis TP. The overall sensitivity and specificity were 98.4% and 99.5%, 97.7% and 97.1%, and 99.2% and 99.7% respectively. The assays are comparable and considered adequate for syphilis screening. Copyright © 2016 Elsevier Inc. All rights reserved.

Cloning and sequencing of V genes from anti-osteosarcoma monoclonal antibodies TP-1 and TP-3: Location of lysine residues and implications for radiolabeling

International Nuclear Information System (INIS)

Olafsen, Tove; Bruland, Oeyvind S.; Zalutsky, Michael R.; Sandlie, Inger

1995-01-01

Monoclonal antibodies TP-1 and TP-3 are of potential utility for the radioimmunodiagnosis of osteosarcoma in both human and canine patients. The V genes of these antibodies were cloned and sequenced and to facilitate radiolabeling of these proteins, the location of the lysine residues within these sequences have been determined. The V-domains of TP-1 contain a total of 12 lysines, 10 in the framework region and 2 in the CDR region, while the V-domains of TP-3 contain a total of 14 lysines, 11 in the framework region and 3 in the CDR regions. Using space-filling models, the availability of each lysine residue for radiolabeling, and potential interference with antigen binding was predicted

Cloning and sequencing of V genes from anti-osteosarcoma monoclonal antibodies TP-1 and TP-3: Location of lysine residues and implications for radiolabeling

Energy Technology Data Exchange (ETDEWEB)

Olafsen, Tove; Bruland, Oeyvind S.; Zalutsky, Michael R.; Sandlie, Inger

1995-08-01

Monoclonal antibodies TP-1 and TP-3 are of potential utility for the radioimmunodiagnosis of osteosarcoma in both human and canine patients. The V genes of these antibodies were cloned and sequenced and to facilitate radiolabeling of these proteins, the location of the lysine residues within these sequences have been determined. The V-domains of TP-1 contain a total of 12 lysines, 10 in the framework region and 2 in the CDR region, while the V-domains of TP-3 contain a total of 14 lysines, 11 in the framework region and 3 in the CDR regions. Using space-filling models, the availability of each lysine residue for radiolabeling, and potential interference with antigen binding was predicted.

RITA displays anti-tumor activity in medulloblastomas independent of TP53 status.

Science.gov (United States)

Gottlieb, Aline; Althoff, Kristina; Grunewald, Laura; Thor, Theresa; Odersky, Andrea; Schulte, Marc; Deubzer, Hedwig E; Heukamp, Lukas; Eggert, Angelika; Schramm, Alexander; Schulte, Johannes H; Künkele, Annette

2017-04-25

Current therapy of medulloblastoma, the most common malignant brain tumor of childhood, achieves 40-70% survival. Secondary chemotherapy resistance contributes to treatment failure, where TP53 pathway dysfunction plays a key role. MDM2 interaction with TP53 leads to its degradation. Reactivating TP53 functionality using small-molecule inhibitors, such as RITA, to disrupt TP53-MDM2 binding may have therapeutic potential. We show here that RITA decreased viability of all 4 analyzed medulloblastoma cell lines, regardless of TP53 functional status. The decrease in cell viability was accompanied in 3 of the 4 medulloblastoma cell lines by accumulation of TP53 protein in the cells and increased CDKN1A expression. RITA treatment in mouse models inhibited medulloblastoma xenograft tumor growth. These data demonstrate that RITA treatment reduces medulloblastoma cell viability in both in vitro and in vivo models, and acts independently of cellular TP53 status, identifying RITA as a potential therapeutic agent to treat medulloblastoma.

WNT activation by lithium abrogates TP53 mutation associated radiation resistance in medulloblastoma.

Science.gov (United States)

Zhukova, Nataliya; Ramaswamy, Vijay; Remke, Marc; Martin, Dianna C; Castelo-Branco, Pedro; Zhang, Cindy H; Fraser, Michael; Tse, Ken; Poon, Raymond; Shih, David J H; Baskin, Berivan; Ray, Peter N; Bouffet, Eric; Dirks, Peter; von Bueren, Andre O; Pfaff, Elke; Korshunov, Andrey; Jones, David T W; Northcott, Paul A; Kool, Marcel; Pugh, Trevor J; Pomeroy, Scott L; Cho, Yoon-Jae; Pietsch, Torsten; Gessi, Marco; Rutkowski, Stefan; Bognár, Laszlo; Cho, Byung-Kyu; Eberhart, Charles G; Conter, Cecile Faure; Fouladi, Maryam; French, Pim J; Grajkowska, Wieslawa A; Gupta, Nalin; Hauser, Peter; Jabado, Nada; Vasiljevic, Alexandre; Jung, Shin; Kim, Seung-Ki; Klekner, Almos; Kumabe, Toshihiro; Lach, Boleslaw; Leonard, Jeffrey R; Liau, Linda M; Massimi, Luca; Pollack, Ian F; Ra, Young Shin; Rubin, Joshua B; Van Meir, Erwin G; Wang, Kyu-Chang; Weiss, William A; Zitterbart, Karel; Bristow, Robert G; Alman, Benjamin; Hawkins, Cynthia E; Malkin, David; Clifford, Steven C; Pfister, Stefan M; Taylor, Michael D; Tabori, Uri

2014-12-24

TP53 mutations confer subgroup specific poor survival for children with medulloblastoma. We hypothesized that WNT activation which is associated with improved survival for such children abrogates TP53 related radioresistance and can be used to sensitize TP53 mutant tumors for radiation. We examined the subgroup-specific role of TP53 mutations in a cohort of 314 patients treated with radiation. TP53 wild-type or mutant human medulloblastoma cell-lines and normal neural stem cells were used to test radioresistance of TP53 mutations and the radiosensitizing effect of WNT activation on tumors and the developing brain. Children with WNT/TP53 mutant medulloblastoma had higher 5-year survival than those with SHH/TP53 mutant tumours (100% and 36.6%±8.7%, respectively (p<0.001)). Introduction of TP53 mutation into medulloblastoma cells induced radioresistance (survival fractions at 2Gy (SF2) of 89%±2% vs. 57.4%±1.8% (p<0.01)). In contrast, β-catenin mutation sensitized TP53 mutant cells to radiation (p<0.05). Lithium, an activator of the WNT pathway, sensitized TP53 mutant medulloblastoma to radiation (SF2 of 43.5%±1.5% in lithium treated cells vs. 56.6±3% (p<0.01)) accompanied by increased number of γH2AX foci. Normal neural stem cells were protected from lithium induced radiation damage (SF2 of 33%±8% for lithium treated cells vs. 27%±3% for untreated controls (p=0.05). Poor survival of patients with TP53 mutant medulloblastoma may be related to radiation resistance. Since constitutive activation of the WNT pathway by lithium sensitizes TP53 mutant medulloblastoma cells and protect normal neural stem cells from radiation, this oral drug may represent an attractive novel therapy for high-risk medulloblastomas.

TP53 mutation spectrum in smokers and never smoking lung cancer patients

Directory of Open Access Journals (Sweden)

Ann Rita Halvorsen

2016-05-01

Full Text Available AbstractBackground: TP53 mutations are among the most common mutations found in lung cancers, identified as an independent prognostic factor in many types of cancers. The purpose of this study was to investigate the frequency and prognostic impact of TP53 mutations in never-smokers and in different histological subtypes of lung cancer.Methods: We analysed tumour tissue from 394 non-small cell carcinomas including adenocarcinomas (n=229, squamous cell carcinomas (n=112, large cell carcinomas (n=30 and others (n=23 for mutations in TP53 by the use of Sanger sequencing (n=394 and next generation sequencing (n=100. Results: TP53 mutations were identified in 47.2% of the samples, with the highest frequency (65% of mutations among squamous cell carcinomas. Among never-smokers, 36% carried a TP53 mutation, identified as a significant independent negative prognostic factor in this subgroup. For large cell carcinomas, a significantly prolonged progression free survival was found for those carrying a TP53 mutation. In addition, the frequency of frameshift mutations was doubled in squamous cell carcinomas (20.3% compared to adenocarcinomas (9.1%.Conclusion: TP53 mutation patterns differ between the histological subgroups of lung cancers, as also influenced by smoking history. This indicates that the histological subtypes in lung cancer are genetically different, and that smoking-induced TP53 mutations may have a different biological impact than TP53 mutations occurring in never-smokers.

Comparative Environmental Life Cycle Assessment of Oxyfuel and Post-combustion Capture with MEA and AMP/PZ - Case Studies from the EDDiCCUT Project

NARCIS (Netherlands)

Oreggioni, Gabriel D.; Singh, Bhawna; Hung, Christine Roxanne; Van Der Spek, Mijndert W.; Skagestad, Ragnhild; Eldrup, Nils Henrik; Ramirez, Andrea; Strømman, Anders Hammer

2017-01-01

This work presents the results of a comparative life cycle assessment study for three CCS technologies applied to a coal-fired power plant: post-combustion capture with MEA, post combustion capture with AMP/PZ and cryogenic oxy-fuel. This study has been performed in the context of the EDDiCCUT

Purification, crystallization and preliminary X-ray analysis of TP0435 (Tp17) from the syphilis spirochete Treponema pallidum

International Nuclear Information System (INIS)

Brautigam, Chad A.; Deka, Ranjit K.; Norgard, Michael V.

2013-01-01

The soluble portion of TP0435, a putative periplasmic lipoprotein from the syphilis spirochete T. pallidum, has been purified and crystallized in a rhombohedral space group. A complete native data set has been collected to 2.4 Å resolution. Syphilis, caused by the bacterial spirochete Treponema pallidum, remains a prominent sexually transmitted infection worldwide. Despite sequencing of the genome of this obligate human pathogen 15 years ago, the functions of a large number of the gene products of T. pallidum are still unknown, particularly with respect to those of the organism’s periplasmic lipoproteins. To better understand their functions, a structural biology approach has been pursued. To this end, the soluble portion of the T. pallidum TP0435 lipoprotein (also known as Tp17) was cloned, hyper-expressed in Escherichia coli and purified to apparent homogeneity. The protein crystals obtained from this preparation diffracted to 2.4 Å resolution and had the symmetry of space group R3. In the hexagonal setting, the unit-cell parameters were a = b = 85.7, c = 85.4 Å

Prediction of Microcystis Blooms Based on TN:TP Ratio and Lake Origin

Directory of Open Access Journals (Sweden)

Yoshimasa Amano

2008-01-01

Full Text Available We evaluated the relationship between TN:TP ratio and Microcystis growth via a database that includes worldwide lakes based on four types of lake origin (dammed, tectonic, coastal, and volcanic lakes. We used microcosm and mesocosm for the nutrient elution tests with lake water and four kinds of sediment (nontreated, MgO sprinkling treated, dissolved air flotation [DAF] treated, and combined treated sediment in order to control TN:TP ratio and to suppress Microcystis growth. Microcystis growth was related to TN:TP ratio, with the maximum value at an optimum TN:TP ratio and the minimum values when the TN:TP ratios reached to 0 or ∞. The kurtosis of the distribution curve varied with the type of lake origin; the lowest kurtosis was found in dammed lakes, while the highest was found in volcanic lakes. The lake trophic state could affect the change in the kurtosis, providing much lower kurtosis at eutrophic lakes (dammed lakes than that at oligotrophic lakes (volcanic lakes. The relationship between TN:TP ratio and Microcystis growth could be explained by the nutrient elution tests under controlled TN:TP ratios through the various sediment treatments. A significant suppression of Microcystis growth of 70% could be achieved when the TN:TP ratios exceeded 21. Lake origin could be regarded as an index including morphological and geographical factors, and controlling the trophic state in lakes. The origin rather than trophic state for lakes could be considered as an important factor of TN:TP influences on Microcystis growth.

TP53 and lacZ mutagenesis induced by 3-nitrobenzanthrone in Xpa-deficient human TP53 knock-in mouse embryo fibroblasts.

Science.gov (United States)

Kucab, Jill E; Zwart, Edwin P; van Steeg, Harry; Luijten, Mirjam; Schmeiser, Heinz H; Phillips, David H; Arlt, Volker M

2016-03-01

3-Nitrobenzanthrone (3-NBA) is a highly mutagenic compound and possible human carcinogen found in diesel exhaust. 3-NBA forms bulky DNA adducts following metabolic activation and induces predominantly G:CT:A transversions in a variety of experimental systems. Here we investigated the influence of nucleotide excision repair (NER) on 3-NBA-induced mutagenesis of the human tumour suppressor gene TP53 and the reporter gene lacZ. To this end we utilised Xpa -knockout (Xpa-Null) human TP53 knock-in (Hupki) embryo fibroblasts (HUFs). As Xpa is essential for NER of bulky DNA adducts, we hypothesized that DNA adducts induced by 3-NBA would persist in the genomes of Xpa-Null cells and lead to an increased frequency of mutation. The HUF immortalisation assay was used to select for cells harbouring TP53 mutations following mutagen exposure. We found that Xpa-Null Hupki mice and HUFs were more sensitive to 3-NBA treatment than their wild-type (Xpa-WT) counterparts. However, following 3-NBA treatment and immortalisation, a similar frequency of TP53-mutant clones arose from Xpa-WT and Xpa-Null HUF cultures. In cells from both Xpa genotypes G:CT:A transversion was the predominant TP53 mutation type and mutations exhibited bias towards the non-transcribed strand. Thirty-two percent of 3-NBA-induced TP53 mutations occurred at CpG sites, all of which are hotspots for mutation in smokers' lung cancer (codons 157, 158, 175, 245, 248, 273, 282). We also examined 3-NBA-induced mutagenesis of an integrated lacZ reporter gene in HUFs, where we again observed a similar mutant frequency in Xpa-WT and Xpa-Null cells. Our findings suggest that 3-NBA-DNA adducts may evade removal by global genomic NER; the persistence of 3-NBA adducts in DNA may be an important factor in its mutagenicity. Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.

Clear Plaque Mutants of Lactococcal Phage TP901-1

DEFF Research Database (Denmark)

Kot, Witold; Kilstrup, Mogens; Vogensen, Finn K.

2016-01-01

We report a method for obtaining turbid plaques of the lactococcal bacteriophage TP901-1 and its derivative TP901-BC1034. We have further used the method to isolate clear plaque mutants of this phage. Analysis of 8 such mutants that were unable to lysogenize the host included whole genome...

[Evaluation of the Performance of Two Kinds of Anti-TP Enzyme-Linked Immunosorbent Assay].

Science.gov (United States)

Gao, Nan; Huang, Li-Qin; Wang, Rui; Jia, Jun-Jie; Wu, Shuo; Zhang, Jing; Ge, Hong-Wei

2018-06-01

To evaluate the accuracy and precision of 2 kinds of anti-treponema pallidum (anti-TP) ELISA reagents in our laboratory for detecting the anti-TP in voluntary blood donors, so as to provide the data support for use of ELISA reagents after introduction of chemiluminescene immunoassay (CLIA). The route detection of anti-TP was performed by using 2 kinds of ELISA reagents, then 546 responsive positive samples detected by anti-TP ELISA were collected, and the infections status of samples confirmed by treponema pallidum particle agglutination (TPPA) test was identified. The confirmed results of responsive samples detected by 2 kinds of anti-TP ELISA reagents were compared, the accuracy of 2 kinds of anti-TP ELISA reagents was analyzed by drawing ROC and comparing area under curve (AUC), and precision of 2 kinds of anti-TP ELISA reagents was compared by statistical analysis of quality control data from 7.1 2016 to 6.30 2017. There were no statistical difference in confirmed positive rate of responsive samples and weak positive samples between 2 kinds of anti-TP ELISA reagents. The responsive samples detected by 2 kinds of anti-TP ELISA reagents accounted for 85.53%(467/546) of all responsive samples, the positive rate confirmed by TPPA test was 82.87%. 44 responsive samples detected by anti-TP ELISA reagent A and 35 responsive samples detected by anti-TP ELISA reagent B were confirmed to be negative by TPPA test. Comparison of AUC showed that the accuracy of 2 kinds of anti-TP ELISA reagents was more high, the difference between 2 reagents was not statistically significant. The coefficient of variation (CV) of anti-TP ELISA reagent A and B was 14.98% and 18.04% respectively, which met the precision requirement of ELISA test. The accuracy and precision of 2 kinds of anti-TP ELISA reagents used in our laboratory are similar, and using any one of anti-TP ELISA reagents all can satisfy the requirements of blood screening.

Re-appearance of cooperativity in ultra-small spin-crossover [Fe(pz){Ni(CN)₄}] nanoparticles.

Science.gov (United States)

Peng, Haonan; Tricard, Simon; Félix, Gautier; Molnár, Gábor; Nicolazzi, William; Salmon, Lionel; Bousseksou, Azzedine

2014-10-06

A reverse nanoemulsion technique was used for the elaboration of [Fe(pz){Ni(CN)4}] nanoparticles. Low-temperature micellar exchange made it possible to elaborate ultra-small nanoparticles with sizes down to 2 nm. When decreasing the size of the particles from 110 to 12 nm the spin transition shifts to lower temperatures, becomes gradual, and the hysteresis shrinks. On the other hand, a re-opening of the hysteresis was observed for smaller (2 nm) particles. A detailed (57)Fe Mössbauer spectroscopy analysis was used to correlate this unusual phenomenon to the modification of the stiffness of the nanoparticles thanks to the determination of their Debye temperature. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Diagnostic value of recombinant Tp0821 protein in serodiagnosis for syphilis.

Science.gov (United States)

Xie, Y; Xu, M; Wang, C; Xiao, J; Xiao, Y; Jiang, C; You, X; Zhao, F; Zeng, T; Liu, S; Kuang, X; Wu, Y

2016-04-01

Syphilis is a multistage sexually transmitted disease that remains a serious public health concern worldwide. The coexistence of Treponema pallidum with other closely related members of spirochaeta, such as Leptospira spp. and Borrelia burgdorferi, has complicated the serodiagnosis due to cross-reactive antigens. In this study, recombinant Tp0821 protein was expressed in Escherichia coli and purified by metal affinity chromatography. Then enzyme-linked immunosorbent assays (ELISAs) based on Tp0821 for the detection of specific antibodies were established. The relative positive rates of the IgM ELISA and the IgG ELISA were found to be 91·0 and 98·3%, respectively, when screening 578 syphilis specimens. The specificities were 94·3 and 100%, respectively, when cross-checking with serum samples obtained from 30 patients with Lyme disease, five patients with leptospirosis, and 52 uninfected controls. In addition, relative positive rates and specificities of Tp0821 for human sera were all 100% in Western blotting. When compared to the syphilis diagnostic tests commonly used in clinical settings, we found that the results of Tp0821-based ELISAs correlated well with the results of the treponemal tests, specifically the T. pallidum particle agglutination (TP-PA) test and the chemiluminescent immunoassay (CIA). Thus, these findings identify Tp0821 as a novel serodiagnostic candidate for syphilis. In this study, we expressed and purified the Treponema pallidum protein Tp0821 and developed Tp0821-based enzyme-linked immunosorbent assays (ELISAs) for the detection of specific antibodies. The serodiagnostic performance of the recombinant protein was then evaluated. When compared to the results of syphilis diagnostic tests commonly used in clinical settings, we found that the reactivities of syphilitic sera with the recombinant antigen correlated well with the results of the treponemal tests, specifically the T. pallidum particle agglutination (TP-PA) test and the

The IARC TP53 mutation database: a resource for studying the significance of TP53 mutations in human cancers

Directory of Open Access Journals (Sweden)

Magali Olivier

2007-02-01

Full Text Available

The tumor suppressor gene TP53 is frequently inactivated by gene mutations in many types of human sporadic cancers, and inherited TP53 mutations predispose to a wide spectrum of early-onset tumors (Li-Fraumeni et Li-Fraumenilike Syndromes. All TP53 gene variations (somatic and germline mutations, as well as polymorphisms that are reported in the scientific literature or in SNP databases are compiled in the IARC TP53 Database. This database provides structured data and analysis tools to study mutation patterns in human cancers and cell-lines and to investigate the clinical impact of mutations. It contains annotations related to the clinical and pathological characteristics of tumors, as well as the demographics and carcinogen exposure of patients. The IARC TP53 web site (http://www-p53.iarc.fr/ provides a search interface for the core database and includes a comprehensive user guide, a slideshow on TP53 mutations in human cancer, protocols and references for sequencing TP53 gene, and links to relevant publications and bioinformatics databases. The database interface allows download of entire data sets and propose various tools for the selection, analysis and downloads of specific sets of data according to user's query.

Recently, new annotations on the functional properties of mutant p53 proteins have been integrated in this database. Indeed, the most frequent TP53 alterations observed in cancers (75% are missense mutations that result in the production of a mutant protein that differ from the wildtype by one single amino-acid. The characterization of the biological activities of these mutant proteins is thus very important. Over the last ten years, a great amount of systematic data has been generated from experimental assays performed in

Alterations of the TP53 Gene in Gastric and Esophageal Carcinogenesis

Directory of Open Access Journals (Sweden)

Marilanda Ferreira Bellini

2012-01-01

Full Text Available TP53 genes is one of more important tumor suppressor gene, which acts as a potent transcription factor with fundamental role in the maintenance of genetic stability. The development of esophageal and gastric cancers is a multistep process resulting in successive accumulation of genetic alterations that culminates in the malignant transformation. Thus, this study highlights the participation of the main genetic alterations of the TP53 gene in esophageal and gastric carcinogenesis. Among these changes, high frequency of TP53 mutations, loss of heterozygosity (LOH, overexpression of the p53 protein, and consequently loss of p53 function, which would be early events in esophageal and gastric cancers, as well as an important biomarker of the prognosis and treatment response. Furthermore, Single Nucleotide Polymorphisms (SNPs of TP53 have been implicated in the development and prognosis of several cancers, mainly TP53 codon 72 polymorphism whose role has been extensively studied in relation to susceptibility for esophageal and gastric cancer development.

Bioinformatics and phylogenetic analysis of human Tp73 gene ...

African Journals Online (AJOL)

The Tp73 gene encoding p73 protein belongs to the Tp53 gene family and it functions in the initiation of cell-cycle arrest or apoptosis and also involves in regulating a series of pathways including breast cancer, neuroblastoma and cholorectal cancer. New discoveries about the control and function of p73 are still in progress ...

Protein - TP Atlas | LSDB Archive [Life Science Database Archive metadata

Lifescience Database Archive (English)

Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data ...p_atlas_protein.zip File URL: ftp://ftp.biosciencedbc.jp/archive/tp_atlas/LATEST/...story of This Database Site Policy | Contact Us Protein - TP Atlas | LSDB Archive ...

Tp53 gene mediates distinct dopaminergic neuronal damage in different dopaminergic neurotoxicant models

Directory of Open Access Journals (Sweden)

Tao Lu

2017-01-01

Full Text Available Tp53, a stress response gene, is involved in diverse cell death pathways and its activation is implicated in the pathogenesis of Parkinson's disease. However, whether the neuronal Tp53 protein plays a direct role in regulating dopaminergic (DA neuronal cell death or neuronal terminal damage in different neurotoxicant models is unknown. In our recent studies, in contrast to the global inhibition of Tp53 function by pharmacological inhibitors and in traditional Tp53 knock-out mice, we examined the effects of DA-specific Tp53 gene deletion after 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and methamphetamine exposure. Our data suggests that the Tp53 gene might be involved in both neuronal apoptosis and neuronal terminal damage caused by different neurotoxicants. Additional results from other studies also suggest that as a master regulator of many pathways that regulate apoptosis and synaptic terminal damage, it is possible that Tp53 may function as a signaling hub to integrate different signaling pathways to mediate distinctive target pathways. Tp53 protein as a signaling hub might be able to evaluate the microenvironment of neurons, assess the forms and severities of injury incurred, and determine whether apoptotic cell death or neuronal terminal degeneration occurs. Identification of the precise mechanisms activated in distinct neuronal damage caused by different forms and severities of injuries might allow for development of specific Tp53 inhibitors or ways to modulate distinct downstream target pathways involved.

Low Prevalence of TP53 Mutations and MDM2 Amplifications in Pediatric Rhabdomyosarcoma

Directory of Open Access Journals (Sweden)

Simona Ognjanovic

2012-01-01

Full Text Available The tumor suppressor gene TP53 is the most commonly mutated gene in human cancer. The reported prevalence of mutations in rhabdomyosarcoma (RMS varies widely, with recent larger studies suggesting that TP53 mutations in pediatric RMS may be extremely rare. Overexpression of MDM2 also attenuates p53 function. We have performed TP53 mutation/MDM2 amplification analyses in the largest series analyzed thus far, including DNA isolated from 37 alveolar and 38 embryonal RMS tumor samples obtained from the Cooperative Human Tissue Network (CHTN. Available samples were frozen tumor tissues (N=48 and histopathology slides. TP53 mutations in exons 4–9 were analyzed by direct sequencing in all samples, and MDM2 amplification analysis was performed by differential PCR on a subset of 22 samples. We found only one sample (1/75, 1.3% carrying a TP53 mutation at codon 259 (p.D259Y and no MDM2 amplification. Two SNPs in the TP53 pathway, associated with accelerated tumor onset in germline TP53 mutation carriers, (TP53 SNP72 (rs no. 1042522 and MDM2 SNP309 (rs no. 2279744, were not found to confer earlier tumor onset. In conclusion, we confirm the extremely low prevalence of TP53 mutations/MDM2 amplifications in pediatric RMS (1.33% and 0%, respectively. The possible inactivation of p53 function by other mechanisms thus remains to be elucidated.

TP53 dysfunction in CLL: Implications for prognosis and treatment

NARCIS (Netherlands)

te Raa, Gera D.; Kater, Arnon P.

2016-01-01

Despite the availability of novel targeted agents, TP53 defects remain the most important adverse prognostic factor in chronic lymphocytic leukemia (CLL). Detection of deletion of TP53 locus (17p deletion) by fluorescent in situ hybridization (FISH) has become standard and performed prior to every

Matematikaoktatás a bolognai típusú gazdasági képzésekben (Maths instruction in Bologna-type economics tuition)

OpenAIRE

Kánnai, Zoltán; Pintér, Miklós; Tasnádi, Attila

2010-01-01

A gazdálkodási és közgazdasági alap- és mesterszakok tanterveiben számos matematikai tárgy szerepel. A kétciklusú bolognai képzésre történő áttérés Európa-szerte igényelte a matematikaoktatás szerkezetének, mennyiségének és mélységének újragondolását a gazdálkodási és közgazdasági képzési területeken. Mivel az átállás országonként és intézményenként párhuzamosan és jelentős mértékben egymástól függetlenül ment végbe, egyetemenként számos eltérő megoldással találkozhatunk. Az egyetemek operatí...

Limited importance of the dominant-negative effect of TP53 missense mutations

International Nuclear Information System (INIS)

Stoczynska-Fidelus, Ewelina; Liberski, Pawel P; Rieske, Piotr; Szybka, Malgorzata; Piaskowski, Sylwester; Bienkowski, Michal; Hulas-Bigoszewska, Krystyna; Banaszczyk, Mateusz; Zawlik, Izabela; Jesionek-Kupnicka, Dorota; Kordek, Radzislaw

2011-01-01

Heterozygosity of TP53 missense mutations is related to the phenomenon of the dominant-negative effect (DNE). To estimate the importance of the DNE of TP53 mutations, we analysed the percentage of cancer cases showing a single heterozygous mutation of TP53 and searched for a cell line with a single heterozygous mutation of this gene. This approach was based on the knowledge that genes with evident DNE, such as EGFR and IDH1, represent nearly 100% of single heterozygous mutations in tumour specimens and cell lines. Genetic analyses (LOH and sequencing) performed for early and late passages of several cell lines originally described as showing single heterozygous TP53 mutations (H-318, G-16, PF-382, MOLT-13, ST-486 and LS-123). Statistical analysis of IARC TP53 and SANGER databases. Genetic analyses of N-RAS, FBXW7, PTEN and STR markers to test cross-contamination and cell line identity. Cell cloning, fluorescence-activated cell sorting and SSCP performed for the PF-382 cell line. A database study revealed TP53 single heterozygous mutations in 35% of in vivo (surgical and biopsy) samples and only 10% of cultured cells (in vitro), although those numbers appeared to be overestimated. We deem that published in vivo TP53 mutation analyses are not as rigorous as studies in vitro, and we did not find any cell line showing a stable, single heterozygous mutation. G16, PF-382 and MOLT-13 cells harboured single heterozygous mutations temporarily. ST-486, H-318 and LS-123 cell lines were misclassified. Specific mutations, such as R175H, R273H, R273L or R273P, which are reported in the literature to exert a DNE, showed the lowest percentage of single heterozygous mutations in vitro (about 5%). We suggest that the currently reported percentage of TP53 single heterozygous mutations in tumour samples and cancer cell lines is overestimated. Thus, the magnitude of the DNE of TP53 mutations is questionable. This scepticism is supported by database investigations showing that retention

Polynomially Riesz elements | Živković-Zlatanović | Quaestiones ...

African Journals Online (AJOL)

A Banach algebra element ɑ ∈ A is said to be "polynomially Riesz", relative to the homomorphism T : A → B, if there exists a nonzero complex polynomial p(z) such that the image Tp ∈ B is quasinilpotent. Keywords: Homomorphism of Banach algebras, polynomially Riesz element, Fredholm spectrum, Browder element, ...

Pediatric oncologist willingness to offer germline TP53 testing in osteosarcoma.

Science.gov (United States)

Shaul, Eliana; Roth, Michael; Lo, Yungtai; Geller, David S; Hoang, Bang; Yang, Rui; Malkin, David; Gorlick, Richard; Gill, Jonathan

2018-03-15

Li-Fraumeni syndrome (LFS) is a cancer predisposition syndrome caused by mutations in the tumor-suppressor gene TP53. Osteosarcoma is a sentinel cancer in LFS. Prior studies using Sanger sequencing platforms have demonstrated that 3% of individuals with osteosarcoma harbor a mutation in TP53. New data from next-generation sequencing have demonstrated that 3.8% of patients with osteosarcoma have a known pathogenic variant, and an additional 5.7% carry exonic variants of unknown significance in TP53. Pediatric oncologists were e-mailed an anonymous 18-question survey assessing their willingness to offer TP53 germline testing to a child with osteosarcoma with or without a family history, and they were evaluated for changes in their choices with the prior data and the new data. One hundred seventy-seven pediatric oncologists (22%) responded to the survey. Respondents were more likely to offer TP53 testing to a patient with a positive family history (77.4% vs 12.4%; P offer TP53 testing once they were provided with the new data (25.4% vs 12.4%; P = .0038). The proportion of providers who responded that they were unsure increased significantly when they were presented with the new data (25.4% vs 10.2%; P = .0002). Potential implications for other family members and the possibility that surveillance imaging would detect new malignancies at an earlier stage were important factors influencing a provider's decision to offer TP53 testing. Recent data increase the proportion of providers willing to offer testing, and this suggests concern on the part of pediatric oncologists that variants of unknown significance may be disease-defining in rare cancers. Cancer 2018;124:1242-50. © 2018 American Cancer Society. © 2018 American Cancer Society.

TP53inp1 Gene Is Implicated in Early Radiation Response in Human Fibroblast Cells

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Nikolett Sándor

2015-10-01

Full Text Available Tumor protein 53-induced nuclear protein-1 (TP53inp1 is expressed by activation via p53 and p73. The purpose of our study was to investigate the role of TP53inp1 in response of fibroblasts to ionizing radiation. γ-Ray radiation dose-dependently induces the expression of TP53inp1 in human immortalized fibroblast (F11hT cells. Stable silencing of TP53inp1 was done via lentiviral transfection of shRNA in F11hT cells. After irradiation the clonogenic survival of TP53inp1 knockdown (F11hT-shTP cells was compared to cells transfected with non-targeting (NT shRNA. Radiation-induced senescence was measured by SA-β-Gal staining and autophagy was detected by Acridine Orange dye and microtubule-associated protein-1 light chain 3 (LC3B immunostaining. The expression of TP53inp1, GDF-15, and CDKN1A and alterations in radiation induced mitochondrial DNA deletions were evaluated by qPCR. TP53inp1 was required for radiation (IR induced maximal elevation of CDKN1A and GDF-15 expressions. Mitochondrial DNA deletions were increased and autophagy was deregulated following irradiation in the absence of TP53inp1. Finally, we showed that silencing of TP53inp1 enhances the radiation sensitivity of fibroblast cells. These data suggest functional roles for TP53inp1 in radiation-induced autophagy and survival. Taken together, we suppose that silencing of TP53inp1 leads radiation induced autophagy impairment and induces accumulation of damaged mitochondria in primary human fibroblasts.

Analisis Jaringan VPN Menggunakan PPTP dan L2TP

Directory of Open Access Journals (Sweden)

Syariful Ikhwan

2017-08-01

Full Text Available VPN adalah teknologi yang membuat jaringan private (pribadi dengan menggunakan jaringan publik agar proses pertukaran data menjadi aman. Teknologi VPN biasanya diterapkan untuk koneksi antara kantor pusat dan kantor cabang. Dinhubkominfo Kabupaten Banyumas sebagai tempat penelitian memiliki beberapa kantor cabang (SKPD. Data yang dipertukarkan antar kantor cabang pada Dinhubkominfo terdiri dari beberapa jenis, namun pada penelitian ini hanya difokuskan pada pertukaran layanan FTP. Teknologi vpn yang saat ini digunakan pada jaringan Dinhubkominfo adalah PPTP. Akan tetapi, penggunaan teknologi tersebut belum tersebut masih belum diketahui tingkat performansi jaringan dibandingkan dengan penggunaan teknologi vpn yang lain. Pada penelitian ini akan dibandingkan penggunaan dua teknologi vpn yang berbeda yaitu antara PPTP dan L2TP, dimana parameter yang digunakan adalah throughput, delay, jitter, dan packet loss. Proses pengambilan data dilakukan dengan menambahkan beban trafik sebesar 512 kbps, 1024 kbps, dan 2048 kbps. Dari hasil penelitian diperoleh data bahwa, rata-rata nilai Delay pada L2TP lebih besar hingga 41% dibanding saat menggunakan PPTP, rata-rata Throughput PPTP naik hingga 34% dibandingkan L2TP, Rata-rata Jitter pada PPTP lebih besar hingga 44% dibandingkan L2TP, sedangkan packet loss yang terjadi pada masing-masing layanan vpn adalah 0

Enhancement of spin polarization induced by Coulomb on-site repulsion between localized pz electrons in graphene embedded with line defects.

Science.gov (United States)

Ren, Ji-Chang; Wang, Zhigang; Zhang, Rui-Qin; Ding, Zejun; Van Hove, Michel A

2015-11-11

It is well known that the effect of Coulomb on-site repulsion can significantly alter the physical properties of the systems that contain localized d and/or f electrons. However, little attention has been paid to the Coulomb on-site repulsion between localized p electrons. In this study, we demonstrated that Coulomb on-site repulsion between localized pz electrons also plays an important role in graphene embedded with line defects. It is shown that the magnetism of the system largely depends on the choice of the effective Coulomb on-site parameter Ueff. Ueff at the edges of the defect enhances the exchange splitting, which increases the magnetic moment and stabilizes a ferromagnetic state of the system. In contrast, Ueff at the center of the defect weakens the spin polarization of the system. The behavior of the magnetism is explained with the Stoner criterion and the charge accumulation at the edges of the defect. Based on the linear response approach, we estimate reasonable values of Ueff to be 2.55 eV (2.3 eV) at the center (edges) of the defects. More importantly, using a DFT+U+J method, we find that exchange interactions between localized p electrons also play an important role in the spin polarization of the system. These results imply that Coulomb on-site repulsion is necessary to describe the strong interaction between localized pz electrons of carbon related materials.

TP508 accelerates fracture repair by promoting cell growth over cell death

International Nuclear Information System (INIS)

Li Xinmin; Wang Hali; Touma, Edward; Qi Yuchen; Rousseau, Emma; Quigg, Richard J.; Ryaby, James T.

2007-01-01

TP508 is a synthetic 23-amino acid peptide representing a receptor-binding domain of human thrombin. We have previously shown that a single injection of TP508 accelerates fracture healing in a rat femoral fracture model. To understand how TP508 acts at the protein level during fracture healing, we compared the translational profiles between saline-control and fractured femur at six time points after TP508 treatment using the second generation of BD Clontech TM Antibody Microarray. Here, we demonstrate that TP508 accelerates fracture healing by modulating expression levels of proteins primarily involved in the functional categories of cell cycle, cellular growth and proliferation, and cell death. The majority of those proteins are physically interrelated and functionally overlapped. The action of those proteins is highlighted by a central theme of promoting cell growth via balance of cell survival over cell death signals. This appears to occur through the stimulation of several bone healing pathways including cell cycle-G1/S checkpoint regulation, apoptosis, JAK/STAT, NF-κB, PDGF, PI3K/AKT, PTEN, and ERK/MAPK

Effects of compositions and Nb-doping on microstructure and piezoelectric properties of PMS-PZ-PT system

Energy Technology Data Exchange (ETDEWEB)

Jiwen, Long; Haiyan, Chen; Zhongyan, Meng

2003-05-25

Sr-substituted (2 mol%) xPMS-(1-x)(PZ-PT) compositions were investigated systematically as a function of PMS concentrations as well as Niobium (Nb) contents. X-ray diffraction (XRD) patterns show that phases shift from tetragonal phase to rhombohedral phase with the increase of PMS concentrations and with the increase of Nb-doping contents in PMS2. The compositions with x=0.05 (PMS2) was found to have superior piezoelectric properties. The properties of the PMS2 compositions were optimized by the Nb-doping contents of 0.1 mol% (d{sub 33}=450 pC N{sup -1}, K{sub p}=0.65, Q{sub m}=1210). The compositions of PMS2 and 0.1 mol% Nb-doped compositions of PMS2 are practically suitable for ultrasonic motor (USM) applications.

Update History of This Database - TP Atlas | LSDB Archive [Life Science Database Archive metadata

Lifescience Database Archive (English)

Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data ...List Contact us TP Atlas Update History of This Database Date Update contents 2013/12/16 The email address i...s ( http://www.tanpaku.org/tpatlas/ ) is opened. About This Database Database Description Download License Update History of Thi...s Database Site Policy | Contact Us Update History of This Database - TP Atlas | LSDB Archive ... ...n the contact information is corrected. 2013/11/19 TP Atlas English archive site is opened. 2008/4/1 TP Atla

TLR4 has a TP53-dependent dual role in regulating breast cancer cell growth.

Science.gov (United States)

Haricharan, Svasti; Brown, Powel

2015-06-23

Breast cancer is a leading cause of cancer-related death, and it is important to understand pathways that drive the disease to devise effective therapeutic strategies. Our results show that Toll-like receptor 4 (TLR4) drives breast cancer cell growth differentially based on the presence of TP53, a tumor suppressor. TP53 is mutationally inactivated in most types of cancer and is mutated in 30-50% of diagnosed breast tumors. We demonstrate that TLR4 activation inhibits growth of TP53 wild-type cells, but promotes growth of TP53 mutant breast cancer cells by regulating proliferation. This differential effect is mediated by changes in tumor cell cytokine secretion. Whereas TLR4 activation in TP53 mutant breast cancer cells increases secretion of progrowth cytokines, TLR4 activation in TP53 wild-type breast cancer cells increases type I IFN (IFN-γ) secretion, which is both necessary and sufficient for mediating TLR4-induced growth inhibition. This study identifies a novel dichotomous role for TLR4 as a growth regulator and a modulator of tumor microenvironment in breast tumors. These results have translational relevance, demonstrating that TP53 mutant breast tumor growth can be suppressed by pharmacologic TLR4 inhibition, whereas TLR4 inhibitors may in fact promote growth of TP53 wild-type tumors. Furthermore, using data generated by The Cancer Genome Atlas consortium, we demonstrate that the effect of TP53 mutational status on TLR4 activity may extend to ovarian, colon, and lung cancers, among others, suggesting that the viability of TLR4 as a therapeutic target depends on TP53 status in many different tumor types.

Phase I study of TP300 in patients with advanced solid tumors with pharmacokinetic, pharmacogenetic and pharmacodynamic analyses

International Nuclear Information System (INIS)

Anthoney, D Alan; Miwa, Masanori; Twelves, Christopher; Evans, TRJ; Naik, Jay; MacPherson, Iain RJ; Crawford, Donna; Hartley, John M; Hartley, Janet A; Saito, Tomohisa; Abe, Masaichi; Jones, Keith

2012-01-01

A Phase I dose escalation first in man study assessed maximum tolerated dose (MTD), dose-limiting toxicity (DLT) and recommended Phase II dose of TP300, a water soluble prodrug of the Topo-1 inhibitor TP3076, and active metabolite, TP3011. Eligible patients with refractory advanced solid tumors, adequate performance status, haematologic, renal, and hepatic function. TP300 was given as a 1-hour i.v. infusion 3-weekly and pharmacokinetic (PK) profiles of TP300, TP3076 and TP3011 were analysed. Polymorphisms in CYP2D6, AOX1 and UGT1A1 were studied and DNA strand-breaks measured in peripheral blood mononuclear cells (PBMCs). 32 patients received TP300 at 1, 2, 4, 6, 8, 10, 12 mg/m 2 . MTD was 10 mg/m 2 ; DLTs at 12 (2/4 patients) and 10 mg/m 2 (3/12) included thrombocytopenia and febrile neutropenia; diarrhoea was uncommon. Six patients (five had received irinotecan), had stable disease for 1.5-5 months. TP3076 showed dose proportionality in AUC and C max from 1–10 mg/m 2 . Genetic polymorphisms had no apparent influence on exposure. DNA strand-breaks were detected after TP300 infusion. TP300 had predictable hematologic toxicity, and diarrhoea was uncommon. AUC at MTD is substantially greater than for SN38. TP3076 and TP3011 are equi-potent with SN38, suggesting a PK advantage. EU-CTR2006-001345-33

CNPY2 promoted the proliferation of renal cell carcinoma cells and increased the expression of TP53

International Nuclear Information System (INIS)

Taniguchi, Hidefumi; Ito, Saya; Ueda, Takashi; Morioka, Yukako; Kayukawa, Naruhiro; Ueno, Akihisa; Nakagawa, Hideo; Fujihara, Atsuko; Ushijima, So; Kanazawa, Motohiro; Hongo, Fumiya; Ukimura, Osamu

2017-01-01

Renal cell carcinoma (RCC) is the most common type of kidney cancer. However, the mechanisms underlying the progression of the disease are not well understood. The data in this report suggest that canopy FGF signaling regulator 2 (CNPY2) is a promoter of RCC progression. We found that CNPY2 significantly promoted growth of RCC cells and upregulated TP53 gene expression. Although TP53 is widely known as a tumor suppressor, in RCC TP53 promoted tumor cell growth. A typical p53 target gene, CDKN1A, was upregulated by both p53 and CNPY2 in RCC cells, suggesting that CNPY2 increased the expression level of TP53. Consistent with these results, CNPY2 and TP53 expression levels were positively correlated in RCC patients. These findings suggested that CNPY2 promoted cancer cell growth in RCC through regulating TP53 gene expression. - Highlights: • CNPY2 promoted growth of renal cell carcinoma (RCC) cells. • TP53 expression levels were increased by CNPY2 in RCC cells. • Growth of RCC cells was promoted by TP53. • CNPY2 expression positively correlated with TP53 expression in RCC patients.

TLR4 has a TP53-dependent dual role in regulating breast cancer cell growth

OpenAIRE

Haricharan, Svasti; Brown, Powel

2015-01-01

This study fundamentally alters our understanding of how TLR4 drives breast cancer. Although TLR4 was previously considered a tumor promoter, we demonstrate a complex, TP53-dependent role for TLR4 in regulating tumor growth. TP53 is a tumor suppressor commonly inactivated across cancer types. In TP53 wild-type cancer cells, TLR4 activation causes secretion of IFN-γ into the microenvironment, resulting in induction of p21 and inhibition of cell growth. Conversely, TLR4 activation in TP53 mutan...

High resolution melting for mutation scanning of TP53 exons 5–8

International Nuclear Information System (INIS)

Krypuy, Michael; Dobrovic, Alexander; Ahmed, Ahmed Ashour; Etemadmoghadam, Dariush; Hyland, Sarah J; Australian Ovarian Cancer Study Group; Fazio, Anna de; Fox, Stephen B; Brenton, James D; Bowtell, David D

2007-01-01

p53 is commonly inactivated by mutations in the DNA-binding domain in a wide range of cancers. As mutant p53 often influences response to therapy, effective and rapid methods to scan for mutations in TP53 are likely to be of clinical value. We therefore evaluated the use of high resolution melting (HRM) as a rapid mutation scanning tool for TP53 in tumour samples. We designed PCR amplicons for HRM mutation scanning of TP53 exons 5 to 8 and tested them with DNA from cell lines hemizygous or homozygous for known mutations. We assessed the sensitivity of each PCR amplicon using dilutions of cell line DNA in normal wild-type DNA. We then performed a blinded assessment on ovarian tumour DNA samples that had been previously sequenced for mutations in TP53 to assess the sensitivity and positive predictive value of the HRM technique. We also performed HRM analysis on breast tumour DNA samples with unknown TP53 mutation status. One cell line mutation was not readily observed when exon 5 was amplified. As exon 5 contained multiple melting domains, we divided the exon into two amplicons for further screening. Sequence changes were also introduced into some of the primers to improve the melting characteristics of the amplicon. Aberrant HRM curves indicative of TP53 mutations were observed for each of the samples in the ovarian tumour DNA panel. Comparison of the HRM results with the sequencing results revealed that each mutation was detected by HRM in the correct exon. For the breast tumour panel, we detected seven aberrant melt profiles by HRM and subsequent sequencing confirmed the presence of these and no other mutations in the predicted exons. HRM is an effective technique for simple and rapid scanning of TP53 mutations that can markedly reduce the amount of sequencing required in mutational studies of TP53

TMACS Test Procedure TP011: Panalarm Interface

International Nuclear Information System (INIS)

Seghers, R.; Washburn, S.J.

1994-01-01

The TMACS Software Test Procedures translate the project's acceptance criteria into test steps. The TMACS Test Plan (WHC-SD-WM-TP-148) is fulfilled when all Test Cases are approved. This Test Procedure tests the TMACS Panalarm Interface functions

Genetic variation in the TP53 pathway and bladder cancer risk. a comprehensive analysis.

Directory of Open Access Journals (Sweden)

Silvia Pineda

Full Text Available Germline variants in TP63 have been consistently associated with several tumors, including bladder cancer, indicating the importance of TP53 pathway in cancer genetic susceptibility. However, variants in other related genes, including TP53 rs1042522 (Arg72Pro, still present controversial results. We carried out an in depth assessment of associations between common germline variants in the TP53 pathway and bladder cancer risk.We investigated 184 tagSNPs from 18 genes in 1,058 cases and 1,138 controls from the Spanish Bladder Cancer/EPICURO Study. Cases were newly-diagnosed bladder cancer patients during 1998-2001. Hospital controls were age-gender, and area matched to cases. SNPs were genotyped in blood DNA using Illumina Golden Gate and TaqMan assays. Cases were subphenotyped according to stage/grade and tumor p53 expression. We applied classical tests to assess individual SNP associations and the Least Absolute Shrinkage and Selection Operator (LASSO-penalized logistic regression analysis to assess multiple SNPs simultaneously.Based on classical analyses, SNPs in BAK1 (1, IGF1R (5, P53AIP1 (1, PMAIP1 (2, SERINPB5 (3, TP63 (3, and TP73 (1 showed significant associations at p-value≤0.05. However, no evidence of association, either with overall risk or with specific disease subtypes, was observed after correction for multiple testing (p-value≥0.8. LASSO selected the SNP rs6567355 in SERPINB5 with 83% of reproducibility. This SNP provided an OR = 1.21, 95%CI 1.05-1.38, p-value = 0.006, and a corrected p-value = 0.5 when controlling for over-estimation.We found no strong evidence that common variants in the TP53 pathway are associated with bladder cancer susceptibility. Our study suggests that it is unlikely that TP53 Arg72Pro is implicated in the UCB in white Europeans. SERPINB5 and TP63 variation deserve further exploration in extended studies.

Mutations in TP53 tumor suppressor gene in wood dust-related sinonasal cancer

DEFF Research Database (Denmark)

Holmila, Reetta; Bornholdt, Jette; Heikkilä, Pirjo

2010-01-01

The causal role of work-related exposure to wood dust in the development of sinonasal cancer has long been established by numerous epidemiologic studies. To study molecular changes in these tumors, we analyzed TP53 gene mutations in 358 sinonasal cancer cases with or without occupational exposure...... affected the ORs only slightly. Smoking did not influence the occurrence of TP53 mutation; however, it was associated with multiple mutations (p = 0.03). As far as we are aware, this is the first study to demonstrate a high prevalence of TP53 mutation-positive cases in a large collection of sinonasal...... cancers with data on occupational exposure. Our results indicate that mutational mechanisms, in particular TP53 mutations, are associated with work-related exposure to wood dust in sinonasal cancer....

Association of mRNA expression of TP53 and the TP53 codon 72 Arg/Pro gene polymorphism with colorectal cancer risk in Asian population: a bioinformatics analysis and meta-analysis.

Science.gov (United States)

Dong, Zhiyong; Zheng, Longzhi; Liu, Weimin; Wang, Cunchuan

2018-01-01

The relationship between TP53 codon 72 Pro/Arg gene polymorphism and colorectal cancer risk in Asians is still controversial, and this bioinformatics analysis and meta-analysis was performed to assess the associations. The association studies were identified from PubMed, and eligible reports were included. RevMan 5.3.1 software, Oncolnc, cBioPortal, and Oncomine online tools were used for statistical analysis. A random/fixed effects model was used in meta-analysis. The data were reported as risk ratios or mean differences with corresponding 95% CI. We confirmed that TP53 was associated with colorectal cancer, the alteration frequency of TP53 was 53% mutation and 7% deep deletion, and TP53 mRNA expression was different in different types of colorectal cancer based on The Cancer Genome Atlas database. Then, 18 studies were included that examine the association of TP53 codon 72 gene polymorphism with colorectal cancer risk in Asians. The meta-analysis indicated that TP53 Pro allele and Pro/Pro genotype were associated with colorectal cancer risk in Asian population, but Arg/Arg genotype was not (Pro allele: odds ratios [OR]=1.20, 95% CI: 1.06 to 1.35, P =0.003; Pro/Pro genotype: OR=1.39, 95% CI: 1.15 to 1.69, P =0.0007; Arg/Arg genotype: OR=0.86, 95% CI: 0.74 to 1.00, P =0.05). Interestingly, in the meta-analysis of the controls from the population-based studies, we found that TP53 codon 72 Pro/Arg gene polymorphism was associated with colorectal cancer risk (Pro allele: OR=1.33, 95% CI: 1.15 to 1.55, P =0.0002; Pro/Pro genotype: OR=1.61, 95% CI: 1.28 to 2.02, P colorectal cancer, but the different value levels of mRNA expression were not associated with survival rate of colon and rectal cancer. TP53 Pro allele and Pro/Pro genotype were associated with colorectal cancer risk in Asians.

Tumor protein 53-induced nuclear protein 1 (TP53INP1 enhances p53 function and represses tumorigenesis

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Jeyran eShahbazi

2013-05-01

Full Text Available Tumor protein 53-induced nuclear protein 1 (TP53INP1 is a stress-induced p53 target gene whose expression is modulated by transcription factors such as p53, p73 and E2F1. TP53INP1 gene encodes two isoforms of TP53INP1 proteins, TP53INP1α and TP53INP1β, both of which appear to be key elements in p53 function. When associated with homeodomain-interacting protein kinase-2 (HIPK2, TP53INP1 phosphorylates p53 protein at Serine 46, enhances p53 protein stability and its transcriptional activity, leading to transcriptional activation of p53 target genes such as p21, PIG-3 and MDM2, cell growth arrest and apoptosis upon DNA damage stress. The anti-proliferative and pro-apoptotic activities of TP53INP1 indicate that TP53INP1 has an important role in cellular homeostasis and DNA damage response. Deficiency in TP53INP1 expression results in increased tumorigenesis; while TP53INP1 expression is repressed during early stages of cancer by factors such as miR-155. This review aims to summarize the roles of TP53INP1 in blocking tumor progression through p53-dependant and p53-independent pathways, as well as the elements which repress TP53INP1 expression, hence highlighting its potential as a therapeutic target in cancer treatment.

Bilateral wilms tumor with TP53-related anaplasia.

Science.gov (United States)

Popov, Sergey D; Vujanic, Gordan M; Sebire, Neil J; Chagtai, Tasnim; Williams, Richard; Vaidya, Sucheta; Pritchard-Jones, Kathy

2013-01-01

Wilms tumor (WT) with diffuse anaplasia has an unfavorable prognosis and is often (>70%) associated with mutations in the TP53 gene. Although most WTs are unilateral, 5-10% are bilateral, and they are almost always present with nephrogenic rests. The latter are considered a precursor of WT. Two cases of bilateral WTs with nephroblastomatosis, in which anaplastic changes were detected over a period of time, were analyzed using clinical, radiological, histopathological, and molecular-genetic data. TP53 was analyzed by direct sequencing of its full coding sequence and intron-exon boundaries in 11 fragments. DNA was extracted from paraffin-embedded or frozen specimens. High-resolution genomic copy number profiling was carried out by UCL Genomics on the Affymetrix Human Mapping 250K Nsp or Genome-Wide Human SNP Array 6.0 platform. Both cases demonstrated a strong association between the appearance of anaplastic clones and TP53 mutations. Synchronous ganglioneuroma was diagnosed in one case. Our cases are unique as they represent a long disease history and demonstrate the difficulties in managing rare cases of bilateral WT with anaplasia. These cases also emphasize the practical importance of modern molecular-genetic techniques and their clinical application. Moreover, they highlight the issue of the adequate sampling needed in order to gather comprehensive, efficient, and sufficient information about genetic events in a single tumor.

TP-model transformation-based-control design frameworks

CERN Document Server

Baranyi, Péter

2016-01-01

This book covers new aspects and frameworks of control, design, and optimization based on the TP model transformation and its various extensions. The author outlines the three main steps of polytopic and LMI based control design: 1) development of the qLPV state-space model, 2) generation of the polytopic model; and 3) application of LMI to derive controller and observer. He goes on to describe why literature has extensively studied LMI design, but has not focused much on the second step, in part because the generation and manipulation of the polytopic form was not tractable in many cases. The author then shows how the TP model transformation facilitates this second step and hence reveals new directions, leading to powerful design procedures and the formulation of new questions. The chapters of this book, and the complex dynamical control tasks which they cover, are organized so as to present and analyze the beneficial aspect of the family of approaches (control, design, and optimization). Additionally, the b...

Analisis de redes sociales y procesos de influencia en la toma de decision grupal

Directory of Open Access Journals (Sweden)

Jorge Ricardo Vivas

2001-01-01

Full Text Available En el presente trabajo se analizan los procesos de influencia por medio del estudio de las relaciones entre medidas convencionales y estructurales en tareas de toma de decisión grupal. Se exploraron los componentes de conectividad y prestigio como facilitadores del proceso de influencia; la conectividad por medio del flujo global, la participación, la densidad de la red ego centrada y la cohesión o distancia reticular. El prestigio se analizó por medio del status sociométrico, medidas de influencia y centralidad (grado, prestigio y poder y medidas de equivalencia estructural para delimitar roles y posiciones. Se utilizó la prueba Supervivencia en la Luna y participaron 70 alumnos de tercer año de la Carrera de Psicología. Los resultados muestran que existe una asociación significativa entre el conocimiento previo a la tarea y la influencia social medida como contribución al producto grupal, que las medidas de centralidad tienen relación con aspectos de la dinámica grupal y no con la contribución al producto y que los roles varían dentro y fuera de la tarea. La influencia está significativamente asociada a la densidad de la red personal. La interpretación de los resultados sugiere que las redes personales de baja densidad favorecen la capacidad de influencia en la producción grupal y que la alta cohesión y conectividad subgrupal la deterioran. También sugieren que las medidas de centralidad se relacionan con influencia en el tráfico comunicacional pero no con la productividad. Y que el status previo y el obtenido durante la actividad, no se vinculan con la producción.

Significance of TP53 Mutation in Wilms Tumors with Diffuse Anaplasia: A Report from the Children's Oncology Group.

Science.gov (United States)

Ooms, Ariadne H A G; Gadd, Samantha; Gerhard, Daniela S; Smith, Malcolm A; Guidry Auvil, Jaime M; Meerzaman, Daoud; Chen, Qing-Rong; Hsu, Chih Hao; Yan, Chunhua; Nguyen, Cu; Hu, Ying; Ma, Yussanne; Zong, Zusheng; Mungall, Andrew J; Moore, Richard A; Marra, Marco A; Huff, Vicki; Dome, Jeffrey S; Chi, Yueh-Yun; Tian, Jing; Geller, James I; Mullighan, Charles G; Ma, Jing; Wheeler, David A; Hampton, Oliver A; Walz, Amy L; van den Heuvel-Eibrink, Marry M; de Krijger, Ronald R; Ross, Nicole; Gastier-Foster, Julie M; Perlman, Elizabeth J

2016-11-15

To investigate the role and significance of TP53 mutation in diffusely anaplastic Wilms tumors (DAWTs). All DAWTs registered on National Wilms Tumor Study-5 (n = 118) with available samples were analyzed for TP53 mutations and copy loss. Integrative genomic analysis was performed on 39 selected DAWTs. Following analysis of a single random sample, 57 DAWTs (48%) demonstrated TP53 mutations, 13 (11%) copy loss without mutation, and 48 (41%) lacked both [defined as TP53-wild-type (wt)]. Patients with stage III/IV TP53-wt DAWTs (but not those with stage I/II disease) had significantly lower relapse and death rates than those with TP53 abnormalities. In-depth analysis of a subset of 39 DAWTs showed seven (18%) to be TP53-wt: These demonstrated gene expression evidence of an active p53 pathway. Retrospective pathology review of TP53-wt DAWT revealed no or very low volume of anaplasia in six of seven tumors. When samples from TP53-wt tumors known to contain anaplasia histologically were available, abnormal p53 protein accumulation was observed by immunohistochemistry. These data support the key role of TP53 loss in the development of anaplasia in WT, and support its significant clinical impact in patients with residual anaplastic tumor following surgery. These data also suggest that most DAWTs will show evidence of TP53 mutation when samples selected for the presence of anaplasia are analyzed. This suggests that modifications of the current criteria to also consider volume of anaplasia and documentation of TP53 aberrations may better reflect the risk of relapse and death and enable optimization of therapeutic stratification. Clin Cancer Res; 22(22); 5582-91. ©2016 AACR. ©2016 American Association for Cancer Research.

Systemic administration of thrombin peptide TP508 enhances VEGF-stimulated angiogenesis and attenuates effects of chronic hypoxia

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Olszewska-Pazdrak, Barbara; Carney, Darrell H.

2015-01-01

Revascularization of chronic wounds and ischemic tissue is attenuated by endothelial dysfunction and the inability of angiogenic factors to stimulate angiogenesis. We recently showed that TP508, a nonproteolytic thrombin peptide, increases perfusion and NO-dependent vasodilation in hearts with chronic ischemia and stimulates NO production by endothelial cells. In this study, we investigated systemic in vivo effects of TP508 on VEGF-stimulated angiogenesis in vitro using aortic explants in normoxic and hypoxic conditions. Mice were injected with saline or TP508 and 24h later aortas were removed and cultured to quantify endothelial sprouting. TP508 injection increased endothelial sprouting and potentiated the in vitro response to VEGF. Exposure of control explants to hypoxia inhibited basal and VEGF-stimulated endothelial cell sprouting. This effect of hypoxia was significantly prevented by TP508 injection. Thus, TP508 systemic administration increases responsiveness of aortic endothelial cells to VEGF and diminishes the effect of chronic hypoxia on endothelial cell sprouting. Studies using human endothelial cells in culture suggest that protective effects of TP508 during hypoxia may involve stimulation of endothelial cell NO production. These data suggest potential clinical benefit of using a combination of systemic TP508 and local VEGF as a therapy for revascularization of ischemic tissue. PMID:23594718

TP53 status and taxane-platinum versus platinum-based therapy in ovarian cancer patients: A non-randomized retrospective study

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Markowska Janina

2008-01-01

Full Text Available Abstract Background Taxane-platinum therapy (TP has replaced platinum-based therapy (PC or PAC, DNA damaging chemotherapy in the postoperative treatment of ovarian cancer patients; however, it is not always effective. TP53 protein plays a differential role in response to DNA-damaging agents and taxanes. We sought to define profiles of patients who benefit the most from TP and also of those who can be treated with PC. Methods We compared the effectiveness of PC/PAC (n = 253 and TP (n = 199 with respect to tumor TP53 accumulation in ovarian cancer patients with FIGO stage IIB-IV disease; this was a non-randomized retrospective study. Immunohistochemical analysis was performed on 452 archival tumors; univariate and multivariate analysis by the Cox's and logistic regression models was performed in all patients and in subgroups with [TP53(+] and without TP53 accumulation [TP53(-]. Results The advantage of taxane-platinum therapy over platinum-based therapy was seen in the TP53(+, and not in the TP53(- group. In the TP53(+ group taxane-platinum therapy enhanced the probability of complete remission (p = .018, platinum sensitivity (p = .014, platinum highly sensitive response (p = .038 and longer survival (OS, p = .008. Poor tumor differentiation diminished the advantage from taxane-platinum therapy in the TP53(+ group. In the TP53(- group PC/PAC was at least equally efficient as taxane-platinum therapy and it enhanced the chance of platinum highly sensitive response (p = .010. However, in the TP53(- group taxane-platinum therapy possibly diminished the risk of death in patients over 53 yrs (p = .077. Among factors that positively interacted with taxane-platinum therapy in some analyses were endometrioid and clear cell type, FIGO III stage, bulky residual tumor, more advanced age of patient and moderate tumor differentiation. Conclusion Our results suggest that taxane-platinum therapy is particularly justified in patients with TP53(+ tumors or older

Significance of TP53 Mutation in Wilms Tumors with Diffuse Anaplasia: A Report from the Children’s Oncology Group

Science.gov (United States)

Ooms, Ariadne H.A.G.; Gadd, Samantha; Gerhard, Daniela S.; Smith, Malcolm A.; Guidry Auvil, Jaime M.; Meerzaman, Daoud; Chen, Qing-Rong; Hsu, Chih Hao; Yan, Chunhua; Nguyen, Cu; Hu, Ying; Ma, Yussanne; Zong, Zusheng; Mungall, Andrew J.; Moore, Richard A.; Marra, Marco A.; Huff, Vicki; Dome, Jeffrey S.; Chi, Yueh-Yun; Tian, Jing; Geller, James I.; Mullighan, Charles G.; Ma, Jing; Wheeler, David A.; Hampton, Oliver A.; Walz, Amy L.; van den Heuvel-Eibrink, Marry M.; de Krijger, Ronald R.; Ross, Nicole; Gastier-Foster, Julie M.; Perlman, Elizabeth J.

2016-01-01

Purpose To investigate the role and significance of TP53 mutation in diffusely anaplastic Wilms tumor (DAWT). Experimental Design All DAWTs registered on National Wilms Tumor Study-5 (n=118) with available samples were analyzed for TP53 mutations and copy loss. Integrative genomic analysis was performed on 39 selected DAWTs. Results Following analysis of a single random sample, 57 DAWT (48%) demonstrated TP53 mutations, 13(11%) copy loss without mutation, and 48(41%) lacked both (defined as TP53-wildtype (wt)). Patients with Stage III/IV TP53-wt DAWTs (but not those with Stage I/II disease) had significantly lower relapse and death rates than those with TP53 abnormalities. In-depth analysis of a subset of 39 DAWT showed 7(18%) to be TP53-wt: these demonstrated gene expression evidence of an active p53 pathway. Retrospective pathology review of TP53-wt DAWT revealed no or very low volume of anaplasia in 6/7 tumors. When samples from TP53-wt tumors known to contain anaplasia histologically were available, abnormal p53 protein accumulation was observed by immunohistochemistry. Conclusion These data support the key role of TP53 loss in the development of anaplasia in WT, and support its significant clinical impact in patients with residual anaplastic tumor following surgery. These data also suggest that most DAWTs will show evidence of TP53 mutation when samples selected for the presence of anaplasia are analyzed. This suggests that modifications of the current criteria to also consider volume of anaplasia and documentation of TP53 aberrations may better reflect the risk of relapse and death and enable optimization of therapeutic stratification. PMID:27702824

TP53 codon 72 polymorphism in pigmentary phenotypes

Indian Academy of Sciences (India)

2012-01-20

Jan 20, 2012 ... pigmentation by acting as a transcription factor for other genes that are ... skin phototype I-II, burns after exposure to UVR and the development of .... morphisms of TP53 codon 72 with breast carcinoma risk: evidence from ...

TP53 mutational status is a potential marker for risk stratification in Wilms tumour with diffuse anaplasia.

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Mariana Maschietto

Full Text Available The presence of diffuse anaplasia in Wilms tumours (DAWT is associated with TP53 mutations and poor outcome. As patients receive intensified treatment, we sought to identify whether TP53 mutational status confers additional prognostic information.We studied 40 patients with DAWT with anaplasia in the tissue from which DNA was extracted and analysed for TP53 mutations and 17p loss. The majority of cases were profiled by copy number (n = 32 and gene expression (n = 36 arrays. TP53 mutational status was correlated with patient event-free and overall survival, genomic copy number instability and gene expression profiling.From the 40 cases, 22 (55% had TP53 mutations (2 detected only after deep-sequencing, 20 of which also had 17p loss (91%; 18 (45% cases had no detectable mutation but three had 17p loss. Tumours with TP53 mutations and/or 17p loss (n = 25 had an increased risk of recurrence as a first event (p = 0.03, hazard ratio (HR, 3.89; 95% confidence interval (CI, 1.26-16.0 and death (p = 0.04, HR, 4.95; 95% CI, 1.36-31.7 compared to tumours lacking TP53 abnormalities. DAWT carrying TP53 mutations showed increased copy number alterations compared to those with wild-type, suggesting a more unstable genome (p = 0.03. These tumours showed deregulation of genes associated with cell cycle and DNA repair biological processes.This study provides evidence that TP53 mutational analysis improves risk stratification in DAWT. This requires validation in an independent cohort before clinical use as a biomarker.

TP53 mutational status is a potential marker for risk stratification in Wilms tumour with diffuse anaplasia.

Science.gov (United States)

Maschietto, Mariana; Williams, Richard D; Chagtai, Tasnim; Popov, Sergey D; Sebire, Neil J; Vujanic, Gordan; Perlman, Elizabeth; Anderson, James R; Grundy, Paul; Dome, Jeffrey S; Pritchard-Jones, Kathy

2014-01-01

The presence of diffuse anaplasia in Wilms tumours (DAWT) is associated with TP53 mutations and poor outcome. As patients receive intensified treatment, we sought to identify whether TP53 mutational status confers additional prognostic information. We studied 40 patients with DAWT with anaplasia in the tissue from which DNA was extracted and analysed for TP53 mutations and 17p loss. The majority of cases were profiled by copy number (n = 32) and gene expression (n = 36) arrays. TP53 mutational status was correlated with patient event-free and overall survival, genomic copy number instability and gene expression profiling. From the 40 cases, 22 (55%) had TP53 mutations (2 detected only after deep-sequencing), 20 of which also had 17p loss (91%); 18 (45%) cases had no detectable mutation but three had 17p loss. Tumours with TP53 mutations and/or 17p loss (n = 25) had an increased risk of recurrence as a first event (p = 0.03, hazard ratio (HR), 3.89; 95% confidence interval (CI), 1.26-16.0) and death (p = 0.04, HR, 4.95; 95% CI, 1.36-31.7) compared to tumours lacking TP53 abnormalities. DAWT carrying TP53 mutations showed increased copy number alterations compared to those with wild-type, suggesting a more unstable genome (p = 0.03). These tumours showed deregulation of genes associated with cell cycle and DNA repair biological processes. This study provides evidence that TP53 mutational analysis improves risk stratification in DAWT. This requires validation in an independent cohort before clinical use as a biomarker.

Autophagy is induced through the ROS-TP53-DRAM1 pathway in response to mitochondrial protein synthesis inhibition.

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Xie, Xiaolei; Le, Li; Fan, Yanxin; Lv, Lin; Zhang, Junjie

2012-07-01

Mitoribosome in mammalian cells is responsible for synthesis of 13 mtDNA-encoded proteins, which are integral parts of four mitochondrial respiratory chain complexes (I, III, IV and V). ERAL1 is a nuclear-encoded GTPase important for the formation of the 28S small mitoribosomal subunit. Here, we demonstrate that knockdown of ERAL1 by RNA interference inhibits mitochondrial protein synthesis and promotes reactive oxygen species (ROS) generation, leading to autophagic vacuolization in HeLa cells. Cells that lack ERAL1 expression showed a significant conversion of LC3-I to LC3-II and an enhanced accumulation of autophagic vacuoles carrying the LC3 marker, all of which were blocked by the autophagy inhibitor 3-MA as well as by the ROS scavenger NAC. Inhibition of mitochondrial protein synthesis either by ERAL1 siRNA or chloramphenicol (CAP), a specific inhibitor of mitoribosomes, induced autophagy in HTC-116 TP53 (+/+) cells, but not in HTC-116 TP53 (-/-) cells, indicating that tumor protein 53 (TP53) is essential for the autophagy induction. The ROS elevation resulting from mitochondrial protein synthesis inhibition induced TP53 expression at transcriptional levels by enhancing TP53 promoter activity, and increased TP53 protein stability by suppressing TP53 ubiquitination through MAPK14/p38 MAPK-mediated TP53 phosphorylation. Upregulation of TP53 and its downstream target gene DRAM1, but not CDKN1A/p21, was required for the autophagy induction in ERAL1 siRNA or CAP-treated cells. Altogether, these data indicate that autophagy is induced through the ROS-TP53-DRAM1 pathway in response to mitochondrial protein synthesis inhibition.

IDH1/IDH2 but Not TP53 Mutations Predict Prognosis in Bulgarian Glioblastoma Patients

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Gergana Stancheva

2014-01-01

Full Text Available Mutations in genes encoding isocitrate dehydrogenase isoforms 1 (IDH1 and 2 (IDH2 have been associated with good prognosis for patients with brain neoplasias and have been commonly found together with mutated TP53 gene. To determine the prevalence of IDH1, IDH2, and TP53 mutations and their impact on overall survival 106 glioblastoma patients were analysed. IDH1 mutations were detected in 13 and IDH2 mutation in one patient. Two homozygous samples with R132H mutation in IDH1 gene and a novel aberration K129R in IDH2 gene were found. Sixty-four percent of IDH1/IDH2 mutated tumours harboured also a mutation in TP53 gene. Genetic aberrations in TP53 were present in 37 patients. Statistical analysis of the impact of the studied factors on the overall survival showed that the mutations in IDH1/IDH2, but not the ones in TP53, were associated with longer survival. Also, the impact of age on prognosis was confirmed. This is the first comprehensive study on glioblastomas in Bulgaria. Our results suggest that IDH1/IDH2 but not TP53 mutations together with other prognostic factors such as age might be applied in clinical practice for prediction of outcome in patients with glioblastomas.

Analysis of full coding sequence of the TP53 gene in invasive vulvar cancers: Implications for therapy.

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Kashofer, Karl; Regauer, Sigrid

2017-08-01

This study evaluates the frequency and type of TP53 gene mutations and HPV status in 72 consecutively diagnosed primary invasive vulvar squamous cell carcinomas (SCC) during the past 5years. DNA of formalin-fixed and paraffin embedded tumour tissue was analysed for 32 HPV subtypes and the full coding sequence of the TP53 gene, and correlated with results of p53 immunohistochemistry. 13/72 (18%) cancers were HPV-induced squamous cell carcinomas, of which 1/13 (8%) carcinoma harboured a somatic TP53 mutation. Among the 59/72 (82%) HPV-negative cancers, 59/72 (82%) SCC were HPV-negative with wild-type gene in 14/59 (24%) SCC and somatic TP53 mutations in 45/59 (76%) SCC. 28/45 (62%) SCC carried one (n=20) or two (n=8) missense mutations. 11/45 (24%) carcinomas showed a single disruptive mutation (3× frame shift, 7× stop codon, 1× deletion), 3/45 SCC a splice site mutation. 3/45 (7%) carcinomas had 2 or 3 different mutations. 18 different "hot spot" mutations were observed in 22/45 cancers (49%; 5× R273, 3× R282; 2× each Y220, R278, R248). Immunohistochemical p53 over expression was identified in most SCC with missense mutations, but not in SCC with disruptive TP53 mutations or TP53 wild-type. 14/45 (31%) patients with TP53 mutated SCC died of disease within 12months (range 2-24months) versus 0/13 patients with HPV-induced carcinomas and 0/14 patients with HPV-negative, TP53 wild-type carcinomas. 80% of primary invasive vulvar SCC were HPV-negative carcinomas with a high frequency of disruptive mutations and "hot spot" TP53 gene mutations, which have been linked to chemo- and radioresistance. The death rate of patients with p53 mutated vulvar cancers was 31%. Immunohistochemical p53 over expression could not reliably identify SCC with TP53 gene mutation. Pharmacological therapies targeting mutant p53 will be promising strategies for personalized therapy in patients with TP53 mutated vulvar cancers. Copyright © 2017. Published by Elsevier Inc.

Use of the integration elements encoded by the temperate lactococcal bacteriophage TP901-1

DEFF Research Database (Denmark)

Brøndsted, Lone; Hammer, Karin

1999-01-01

Previously we showed that only one phage-expressed protein (Orf1), a 425-bp region upstream of the orf1 gene (presumably encoding a promoter), and the attP region are necessary and also sufficient for integration of the bacteriophage TP901-1 genome into the chromosome of Lactococcus lactis subsp......P region seem to be necessary for site-specific integration of the temperate bacteriophage TP901-1. By use of the integrative elements (attP and orf1) expressed by the temperate lactococcal bacteriophage TP901-1, a system for obtaining stable chromosomal single-copy transcriptional fusions in L. lactis...

Germline TP53 mutations and single nucleotide polymorphisms in children Mutaciones y polimorfismos de un único nucleótido del gen TP53 en línea germinal en niños

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Pamela Valva

2009-02-01

Full Text Available Mutations in the gene TP53, which codifies the tumor suppressor protein p53, are found in about 50% of tumors. These mutations can occur not only at somatic level, but also in germline. Pediatric cancer patients, mostly with additional family history of malignancy, should be considered as potential TP53 germline mutation carriers. Germline TP53 mutations and polymorphisms have been widely studied to determine their relation with different tumors' pathogenesis. Our aim was to analyze the occurrence frequency of germline TP53 mutations and polymorphisms and to relate these to tumor development in a pediatric series. Peripheral blood mononuclear cell samples from 26 children with solid tumors [PST] and 21 pediatric healthy donors [HD] were analyzed for germline mutations and polymorphisms in TP53 gene spanning from exon 5 to 8 including introns 5 and 7. These PCR amplified fragments were sequenced to determine variations. A heterozygous mutation at codon 245 was found in 1/26 PST and 0/21 HD. Comparative polymorphisms distribution, at position 14181 and 14201(intron 7, between HD and PST revealed a trend of association (p= 0.07 with cancer risk. HD group disclosed a similar polymorphism distribution as published data for Caucasian and Central/South American populations. This is the first study about TP53 variant frequency and distribution in healthy individuals and cancer patients in Argentina.El gen que codifica para la proteína supresora de tumor p53 (TP53 se encuentra mutado en aproximadamente el 50% de los tumores. Estas mutaciones pueden presentarse como somáticas o en línea germinal. Los niños con tumores, sobre todo aquellos con historia familiar de enfermedad oncológica, deben considerarse potenciales portadores de mutaciones en línea germinal. Las mutaciones de TP53 y los polimorfismos son estudiados para determinar su relación con la patogénesis de diferentes tumores. El objetivo del trabajo fue analizar la frecuencia de

TP53 mutations in clinically normal mucosa adjacent to oral carcinomas

DEFF Research Database (Denmark)

Thode, Christenze; Bilde, Anders; von Buchwald, Christian

2010-01-01

BACKGROUND: The tumour-suppressor protein p53 often accumulates in histologically normal epithelium adjacent to oral squamous cell carcinomas (OSCC). We investigated whether this was associated with mutations in TP53, the gene for p53, and might implicate impending malignancy. METHODS: Specimens...... products were separated by denatured gradient gel electrophoresis. Fragments with a deviant DGEE pattern were sequenced. RESULTS: TP53 mutations were found in six of 18 tumours. Fourteen specimens contained histologically normal mucosa adjacent to the tumour; 13 of these showed small clusters of p53...

Molecular Alterations of TP53 are a Defining Feature of Ovarian High-Grade Serous Carcinoma: A Rereview of Cases Lacking TP53 Mutations in The Cancer Genome Atlas Ovarian Study.

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Vang, Russell; Levine, Douglas A; Soslow, Robert A; Zaloudek, Charles; Shih, Ie-Ming; Kurman, Robert J

2016-01-01

The Cancer Genome Atlas has reported that 96% of ovarian high-grade serous carcinomas (HGSCs) have TP53 somatic mutations suggesting that mutation of this gene is a defining feature of this neoplasm. In the current study, 5 gynecologic pathologists independently evaluated hematoxylin and eosin slides of 14 available cases from The Cancer Genome Atlas classified as HGSC that lacked a TP53 mutation. The histologic diagnoses rendered by these pathologists and the accompanying molecular genetic data are the subject of this report. Only 1 case (Case 5), which contained a homozygous deletion of TP53, had unanimous interobserver agreement for a diagnosis of pure HGSC. In 1 case (Case 3), all 5 observers (100%) rendered a diagnosis of HGSC; however, 3 observers (60%) noted that the histologic features were not classic for HGSC and suggested this case may have arisen from a low-grade serous carcinoma (arisen from an alternate pathway compared with the usual HGSC). In 2 cases (Cases 4 and 12), only 3 observers (60%) in each case, respectively, interpreted it as having a component of HGSC. In the remaining 10 (71%) of tumors (Cases 1, 2, 6-11, 13, and 14), the consensus diagnosis was not HGSC, with individual diagnoses including low-grade serous carcinoma, high-grade endometrioid carcinoma, HGSC, metastatic carcinoma, clear cell carcinoma, atypical proliferative (borderline) serous tumor, and adenocarcinoma, not otherwise specified. Therefore, 13 (93%) of the tumors (Cases 1-4 and 6-14) were either not a pure HGSC or represented a diagnosis other than HGSC, all with molecular results not characteristic of HGSC. Accordingly, our review of the TP53 wild-type HGSCs reported in The Cancer Genome Atlas suggests that 100% of de novo HGSCs contain TP53 somatic mutations or deletions, with the exception of the rare HGSCs that develop from a low-grade serous tumor precursor. We, therefore, propose that lack of molecular alterations of TP53 are essentially inconsistent with the

Fischer-344 Tp53-knockout rats exhibit a high rate of bone and brain neoplasia with frequent metastasis

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Sarah A. Hansen

2016-10-01

Full Text Available Somatic mutations in the Tp53 tumor suppressor gene are the most commonly seen genetic alterations in cancer, and germline mutations in Tp53 predispose individuals to a variety of early-onset cancers. Development of appropriate translational animal models that carry mutations in Tp53 and recapitulate human disease are important for drug discovery, biomarker development and disease modeling. Current Tp53 mouse and rat models have significant phenotypic and genetic limitations, and often do not recapitulate certain aspects of human disease. We used a marker-assisted speed congenic approach to transfer a well-characterized Tp53-mutant allele from an outbred rat to the genetically inbred Fischer-344 (F344 rat to create the F344-Tp53tm1(EGFP-PacQly/Rrrc (F344-Tp53 strain. On the F344 genetic background, the tumor spectrum shifted, with the primary tumor types being osteosarcomas and meningeal sarcomas, compared to the hepatic hemangiosarcoma and lymphoma identified in the original outbred stock model. The Fischer model is more consistent with the early onset of bone and central nervous system sarcomas found in humans with germline Tp53 mutations. The frequency of osteosarcomas in F344-Tp53 homozygous and heterozygous animals was 57% and 36%, respectively. Tumors were highly representative of human disease radiographically and histologically, with tumors found primarily on long bones with frequent pulmonary metastases. Importantly, the rapid onset of osteosarcomas in this promising new model fills a current void in animal models that recapitulate human pediatric osteosarcomas and could facilitate studies to identify therapeutic targets.

A polymorphism (rs1042522) in TP53 gene is a risk factor for Down Syndrome in Sicilian mothers.

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Salemi, Michele; Barone, Concetta; Salluzzo, Maria Grazia; Giambirtone, Mariaconcetta; Scillato, Francesco; Galati Rando, Rosanna; Romano, Carmelo; Morale, Maria Concetta; Ridolfo, Federico; Romano, Corrado

2017-11-01

Trisomy 21 is the most frequent genetic cause of intellectual disability. Tumor Protein 53 (TP53) gene down-regulation triggers chromosomal instability. A TP53 gene polymorphism c.215G > C (rs1042522) is associated with accumulation of aneuploid cells. We analyzed the TP53 c.215G > C (rs1042522) polymorphism in Sicilian mothers of subjects with Down Syndrome (DS) within a case-control study. Nucleotide polymorphism was detected by pyrosequencing technology. The distribution of TP53 c.215G > C polymorphism showed significant difference between mothers of subjects with DS and controls. Our data show that TP53 c.215G > C polymorphism is a risk factor for DS in Sicilian mothers.

Tilapia Piscidin 4 (TP4 Stimulates Cell Proliferation and Wound Closure in MRSA-Infected Wounds in Mice

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Hang-Ning Huang

2015-05-01

Full Text Available Antimicrobial peptides (AMPs are endogenous antibiotics that directly affect microorganisms, and also have a variety of receptor-mediated functions. One such AMP, Tilapia piscidin 4 (TP4, was isolated from Nile tilapia (Oreochromis niloticus; TP4 has antibacterial effects and regulates the innate immune system. The aim of the present study was to characterize the role of TP4 in the regulation of wound closure in mice and proliferation of a keratinocyte cell line (HaCaT and fibroblast cell line (Hs-68. In vitro, TP4 stimulated cell proliferation and activated collagen I, collagen III, and keratinocyte growth factor (KGF gene expression in Hs-68 cells, which induces keratin production by HaCaT cells. This effect was detectable at TP4 concentrations of 6.25 µg/mL in both cell lines. In vivo, TP4 was found to be highly effective at combating peritonitis and wound infection caused by MRSA in mouse models, without inducing adverse behavioral effects or liver or kidney toxicity. Taken together, our results indicate that TP4 enhances the survival rate of mice infected with the bacterial pathogen MRSA through both antimicrobial and wound closure activities mediated by epidermal growth factor (EGF, transforming growth factor (TGF, and vascular endothelial growth factor (VEGF. The peptide is likely involved in antibacterial processes and regulation of tissue homeostasis in infected wounds in mice. Overall, these results suggest that TP4 may be suitable for development as a novel topical agent for wound dressing.

Total Pancreatectomy (TP) and Islet Autotransplantation (IAT) for Chronic Pancreatitis (CP)

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Sutherland, David E.R.; Radosevich, David M.; Bellin, Melena D.; Hering, Bernard J.; Beilman, Gregory J.; Dunn, Ty B.; Chinnakotla, Srinath; Vickers, Selwyn M.; Bland, Barbara; Balamurugan, A.N.; Freeman, Martin L.; Pruett, Timothy L.

2013-01-01

Background Total-pancreatectomy (TP) with intraportal-islet-auto-transplantation (IAT) can relieve pain and preserve beta-cell-mass in patients with chronic-pancreatitis (CP) when other-therapies fail. Reported is a >30-year-single-center-series. Study Design 409 patients (53 children, 5–18 yrs) with CP underwent TP-IAT from Feb/1977–Sept/2011; (etiology idiopathic-41%; SOD/biliary-9%; genetic-14%; divisum-17%; alcohol-7%; other-12%); mean age-35.3 yrs,); 74% female; prior-surgeries 21%--Puestow procedure 9%, Whipple 6%, distal pancreatectomy 7%; other 2%). Islet-function was classified as insulin-independent for those on no insulin; partial if known C-peptide positive or euglycemic on once-daily-insulin; and insulin-dependent if on standard basal–bolus diabetic regimen. An SF-36-survey for Quality-of-Life (QOL)) was completed before and in serial follow-up by patients done since 2007 with an integrated-survey that added in 2008. Results Actuarial-patient-survival post-TP-IAT was 96% in adults and 98% in children (1-year) and; 89% and 98% (5-years). Complications requiring relaparotomy occurred in 15.9%, bleeding (9.5%) being most common. IAT-function is achieved in 90% (C-peptide >0.6 ng/ml). At 3 years, 30% were insulin-independent (25% in adults, 55% in children) and 33% had partial-function. Mean HbA1C was 5000/kg (24%)] correlated with degree of function with insulin-independent rates at 3 yrs of 12, 22 and 72%, partial function 33, 62 and 24%. All patients had pain before TP-IAT and nearly all were on daily-narcotics. After TP-IAT, 85% had pain-improvement. By two years 59% had ceased-narcotics. All children were on narcotics before, 39% at follow-up; pain improved in 94%; 67% became pain-free. In the SF-36 survey, there was significant improvement from baseline in all dimensions including the Physical and Mental Component Summaries (P2/3 of patients with insulin-independence occurring in one-quarter of adults and half the children. PMID:22397977

Alternative electrode placement in (automatic) sleep scoring (Fpz-Cz/Pz-Oz versus C4-A1).

Science.gov (United States)

van Sweden, B; Kemp, B; Kamphuisen, H A; Van der Velde, E A

1990-06-01

The purpose of this study is to investigate whether the international standard electrode placement (C4-A1) can be replaced by an alternative placement (Fpz-Cz/Pz-Oz) in an automatic sleep monitoring system without losing Rechtschaffen and Kales (R-K) balances. Single night-sleep polygraphic recordings of 10 patients, screened in a clinical sleep disorder setting, were recorded simultaneously with both placements, and visual sleep classification was performed separately by two independent observers. Interobserver and interplacement agreement were evaluated by way of average (dis)agreement matrices and kappa values computed for overall and individual stage scoring. Interobserver agreement for both the test and the standard electrode placements and interplacement agreement for both observers were assessed as fair to good or excellent. Scoring differences were evaluated by the rank sign test applied to clinical and theoretical difference scores. It appears that the interplacement differences are about equal to the interobserver differences, except for a slight tendency for sleep to be scored in a deeper stage with the proposed alternative placement. The data are presented and discussed in relation to current literature concepts.

Structural Biology of CI from Phage TP901-1

DEFF Research Database (Denmark)

Rasmussen, Kim Krighaar

(CTD), separated by a linker of various lengths. The NTD is responsible for DNA binding and the CTD is responsible for oligomerization of the CI protein. A Helix-turn-Helix motif is usually responsible for DNA binding. This thesis presents a structural characterization of the CI repressor from TP901...... condition of the CTD, responsible for the arrangement of the hexameric CI repressor. The arrangement of the CI repressor for TP901-1 has previously been described to have 32 point group symmetry based on SAXS studies. The CTD crystallizes with P3121 or P3221 symmetry with one molecule in the asymmetric unit......-length CI can only be answered unambiguously by solving the CTD crystal structure or by use of alternative techniques on the full-length CI....

Synthesis of 75Se-2-phenyl-1,2-benzisoselenazol-3(2H)-one (PZ 51; Ebselen). A novel biologically active organo-selenium compound

International Nuclear Information System (INIS)

Cantineau, R.; Tihange, G.; Plenevaux, A.; Christiaens, L.; Guillaume, M.; Welter, A.; Dereu, N.

1986-01-01

The preparation of 75 Se-ebselen ( 75 Se-PZ 51) in a high radiochemical yield (approx. 40%) and with a specific activity of 240 mCi/mM (8.9 GBq/mM) is described. It entails a very simple, fast and one-pot procedure starting from elemental 75 Se-selenium. 75 Se-diselenosalicylic acid is initially prepared as the key intermediate which is transformed into a corresponding dichloride before reacting with aniline to yield the desired 75 Se-ebselen. Identity and purity of the labelled compound were controlled by comparison in TLC, HPLC and MS with an authentic sample. (author)

Gaining insights into the codon usage patterns of TP53 gene across eight mammalian species.

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Tarikul Huda Mazumder

Full Text Available TP53 gene is known as the "guardian of the genome" as it plays a vital role in regulating cell cycle, cell proliferation, DNA damage repair, initiation of programmed cell death and suppressing tumor growth. Non uniform usage of synonymous codons for a specific amino acid during translation of protein known as codon usage bias (CUB is a unique property of the genome and shows species specific deviation. Analysis of codon usage bias with compositional dynamics of coding sequences has contributed to the better understanding of the molecular mechanism and the evolution of a particular gene. In this study, the complete nucleotide coding sequences of TP53 gene from eight different mammalian species were used for CUB analysis. Our results showed that the codon usage patterns in TP53 gene across different mammalian species has been influenced by GC bias particularly GC3 and a moderate bias exists in the codon usage of TP53 gene. Moreover, we observed that nature has highly favored the most over represented codon CTG for leucine amino acid but selected against the ATA codon for isoleucine in TP53 gene across all mammalian species during the course of evolution.

Human Treponema pallidum 11q/j isolate belongs to subsp. endemicum but contains two loci with a sequence in TP0548 and TP0488 similar to subsp. pertenue and subsp. pallidum, respectively.

Directory of Open Access Journals (Sweden)

Lenka Mikalová

2017-03-01

Full Text Available Treponema pallidum subsp. endemicum (TEN is the causative agent of endemic syphilis (bejel. An unusual human TEN 11q/j isolate was obtained from a syphilis-like primary genital lesion from a patient that returned to France from Pakistan.The TEN 11q/j isolate was characterized using nested PCR followed by Sanger sequencing and/or direct Illumina sequencing. Altogether, 44 chromosomal regions were analyzed. Overall, the 11q/j isolate clustered with TEN strains Bosnia A and Iraq B as expected from previous TEN classification of the 11q/j isolate. However, the 11q/j sequence in a 505 bp-long region at the TP0488 locus was similar to Treponema pallidum subsp. pallidum (TPA strains, but not to TEN Bosnia A and Iraq B sequences, suggesting a recombination event at this locus. Similarly, the 11q/j sequence in a 613 bp-long region at the TP0548 locus was similar to Treponema pallidum subsp. pertenue (TPE strains, but not to TEN sequences.A detailed analysis of two recombinant loci found in the 11q/j clinical isolate revealed that the recombination event occurred just once, in the TP0488, with the donor sequence originating from a TPA strain. Since TEN Bosnia A and Iraq B were found to contain TPA-like sequences at the TP0548 locus, the recombination at TP0548 took place in a treponeme that was an ancestor to both TEN Bosnia A and Iraq B. The sequence of 11q/j isolate in TP0548 represents an ancestral TEN sequence that is similar to yaws-causing treponemes. In addition to the importance of the 11q/j isolate for reconstruction of the TEN phylogeny, this case emphasizes the possible role of TEN strains in development of syphilis-like lesions.

Ibrutinib for previously untreated and relapsed or refractory chronic lymphocytic leukaemia with TP53 aberrations

DEFF Research Database (Denmark)

Farooqui, Mohammed Z H; Valdez, Janet; Martyr, Sabrina

2015-01-01

BACKGROUND: Patients with chronic lymphocytic leukaemia (CLL) with TP53 aberrations respond poorly to first-line chemoimmunotherapy, resulting in early relapse and short survival. We investigated the safety and activity of ibrutinib in previously untreated and relapsed or refractory CLL with TP53...... aberrations. METHODS: In this investigator-initiated, single-arm phase 2 study, we enrolled eligible adult patients with active CLL with TP53 aberrations at the National Institutes of Health Clinical Center (Bethesda, MD, USA). Patients received 28-day cycles of ibrutinib 420 mg orally once daily until...... in one (2%) patient. INTERPRETATION: The activity and safety profile of single-agent ibrutinib in CLL with TP53 aberrations is encouraging and supports its consideration as a novel treatment option for patients with this high-risk disease in both first-line and second-line settings. FUNDING: Intramural...

ELISA with double antigen sandwich for screening specific serum anti-TP antibody in blood donors

International Nuclear Information System (INIS)

Wang Yiqing; Shi Zhixu

2002-01-01

Objective: To select a sensitive and specific laboratory examination suitable for screening serum anti-TP antibody in blood donors. Methods: The serum anti-TP antibody in 11271 blood donors were detected using ELISA with double antigen sandwich and the outcomes were compared with those using RPR assay. The conflicting specimen were confirmed by repeating the test with TPHA assay. Results: The positive rates of serum anti-TP antibody by ELISA with double antigen sandwich and RPR was 0.36% (41/11271) and 0.26% (29/11271), respectively. The coincidence of the detecting outcomes by ELISA with double antigen sandwich and RPR with TPHA was 97.5% (40/41) and 63.41%(26/41) respectively. Conclusion: Compared with RPR assay, ELISA with double antigen sandwich has higher sensibility and specificity for screening serum anti-TP antibody in blood donors

Structure and molecular assignment of lactococcal phage TP901-1 baseplate

DEFF Research Database (Denmark)

Bebeacua, Cecilia; Bron, Patrick; Lai, Livia

2010-01-01

on the host cell surface. We report here the electron microscopic structure of the phage TP901-1 wild-type BP as well as those of two mutants bppL (-) and bppU(-), lacking BppL (the RBPs) or both peripheral BP components (BppL and BppU), respectively. We also achieved an electron microscopic reconstruction...... of a partial BP complex, formed by BppU and BppL. This complex exhibits a tripod shape and is composed of nine BppLs and three BppUs. These structures, combined with light-scattering measurements, led us to propose that the TP901-1 BP harbors six tripods at its periphery, located around the central tube formed...... by ORF46 (Dit) hexamers, at its proximal end, and a ORF47 (Tal) trimer at its distal extremity. A total of 54 BppLs (18 RBPs) are thus available to mediate host anchoring with a large apparent avidity. TP901-1 BP exhibits an infection-ready conformation and differs strikingly from the lactococcal phage p...

Los mecanismos de influencia en el liderazgo

OpenAIRE

Arango Cardona, Patricia

2016-01-01

Este trabajo tiene como objetivo fundamental identificar las tácticas de influencia que utiliza el señor Carlos Pérez, gerente y socio principal de G. & M., en su trato cotidiano con sus colaboradores, así como la reacción de estos últimos ante dichas tácticas.

Knockdown of HSPA9 induces TP53-dependent apoptosis in human hematopoietic progenitor cells.

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Tuoen Liu

Full Text Available Myelodysplastic syndromes (MDS are the most common adult myeloid blood cancers in the US. Patients have increased apoptosis in their bone marrow cells leading to low peripheral blood counts. The full complement of gene mutations that contribute to increased apoptosis in MDS remains unknown. Up to 25% of MDS patients harbor and acquired interstitial deletion on the long arm of chromosome 5 [del(5q], creating haploinsufficiency for a large set of genes including HSPA9. Knockdown of HSPA9 in primary human CD34+ hematopoietic progenitor cells significantly inhibits growth and increases apoptosis. We show here that HSPA9 knockdown is associated with increased TP53 expression and activity, resulting in increased expression of target genes BAX and p21. HSPA9 protein interacts with TP53 in CD34+ cells and knockdown of HSPA9 increases nuclear TP53 levels, providing a possible mechanism for regulation of TP53 by HSPA9 haploinsufficiency in hematopoietic cells. Concurrent knockdown of TP53 and HSPA9 rescued the increased apoptosis observed in CD34+ cells following knockdown of HSPA9. Reduction of HSPA9 below 50% results in severe inhibition of cell growth, suggesting that del(5q cells may be preferentially sensitive to further reductions of HSPA9 below 50%, thus providing a genetic vulnerability to del(5q cells. Treatment of bone marrow cells with MKT-077, an HSPA9 inhibitor, induced apoptosis in a higher percentage of cells from MDS patients with del(5q compared to non-del(5q MDS patients and normal donor cells. Collectively, these findings indicate that reduced levels of HSPA9 may contribute to TP53 activation and increased apoptosis observed in del(5q-associated MDS.

Evaluation of the Lumipulse G TP-N Chemiluminescent Immunoassay as a Syphilis Screening Test.

Science.gov (United States)

Ortiz, Daniel A; Loeffelholz, Michael J

2017-11-01

A syphilis diagnosis is often aided by the detection of treponemal and nontreponemal antibodies. Automated treponemal antibody detection systems enable high-volume clinical laboratories to perform syphilis screening at a faster pace with lower labor costs. The Lumipulse G TP-N chemiluminescent immunoassay is an automated system that qualitatively detects IgG and IgM antibodies against Treponema pallidum antigens in human serum and plasma. To assess performance characteristics and workflow efficiency, the Lumipulse G TP-N assay was compared to the Bioplex 2200 Syphilis IgG multiplex flow immunoassay. Among the 4,134 routine and HIV samples tested by the two automated assays, the percentage of agreement was excellent at 99.0% (95% confidence interval [CI], 98.6% to 99.2%; κ, 0.89), with the Lumipulse G TP-N having a shorter time to first and subsequent results. All specimens with reactive syphilis screening results were further tested by rapid plasma reagin (RPR) and Treponema pallidum particle agglutination (TP·PA) testing ( n = 231). The results from the RPR-reactive samples ( n = 82) showed complete concordance with the two automated assays, while the TP·PA assay displayed some discrepancies. The positive percent agreement (PPA) and negative percent agreement (NPA) between the TP·PA test and the Lumipulse G TP-N test were 98.9% and 77.3%, respectively. The Bioplex 2200 Syphilis IgG immunoassay displayed a similar PPA (100%) but a substantially lower NPA (15.9%). Patient chart reviews of discrepant results suggested that the Lumipulse G TP-N assay produced 27 fewer falsely reactive results and can reduce the amount of additional confirmatory RPR and TP·PA testing needed. The analogous performance characteristics of the two automated systems indicate that the Lumipulse G TP-N assay is suitable for high-throughput syphilis screening. Copyright © 2017 American Society for Microbiology.

Validación del Cuestionario de Creencias Centrales de los Trastornos de la Personalidad (CCE-TP en población colombiana

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Nora Helena Londoño A.

2010-07-01

Full Text Available Se construyó un cuestionario para evaluar creencias centrales asociadas con los trastornos de la personalidad, fundamentadas en el Modelo de la Terapia Cognitiva. Se realizó el análisis estructural y la validez de contenido de la prueba en población universitaria de la ciudad de Medellín, Colombia. La muestra fue representativa y elegida al azar a través de un procedimiento polietápico. Un número de 809 estudiantes universitarios contestaron el cuestionario de creencias centrales de los trastornos de la personalidad (CCE-TP. Se realizó un análisis factorial exploratorio de la prueba, reagrupándose los ítems en 14 factores (F que representan el 61,3% de la varianza. F1: CCE-TP antisocial (8 ítems, a 0,839; F2: CCE-TP esquizotípico/límite (8 ítems, a 0,846; F3: CCE-TP histriónico/patrón seductor (6 ítems, a 0,833; F4: CCE-TP paranoide (6 ítems, a 0,836; F5: CCE-TP por evitación / autopercepción negativa (5 ítems, a 0,755; F6: CCE-TP por dependencia (5 ítems, a 0,797; F7: CCE-TP histriónico/ dependencia emocional (4 ítems, a 0,755; F8: CCETP obsesivo-compulsivo/perfeccionista (4 ítems, a 0,808; F9: CCE-TP por evitación/hipersensible (4 ítems, a 0,766; F10: CCE-TP obsesivo-compulsivo/ crítico frente a los demás (3 ítems, a 0,851; F11: CCE-TP narcisista (4 ítems, a 0,717; F12: CCE-TP pasivo-agresivo / temor a ser dominado (3 ítems, a 0,719; F13: CCE-TP pasivo-agresivo/crítico frente a la autoridad (3 ítems, a 0,685, y F14: CCE-TP esquizoide (2 ítems, a 0,774. El alfa de Cronbach de la prueba fue de 0,931.

Validación del Cuestionario de Creencias Centrales de los Trastornos de la Personalidad (CCE-TP en población colombiana

Directory of Open Access Journals (Sweden)

Diego Castrillón M.

2007-06-01

Full Text Available Se construyó un cuestionario para evaluar creencias centrales asociadas con los trastornos de la personalidad, fundamentadas en el Modelo de la Terapia Cognitiva. Se realizó el análisis estructural y la validez de contenido de la prueba en población universitaria de la ciudad de Medellín, Colombia. La muestra fue representativa y elegida al azar a través de un procedimiento polietápico. Un número de 809 estudiantes universitarios contestaron el cuestionario de creencias centrales de los trastornos de la personalidad (CCE-TP. Se realizó un análisis factorial exploratorio de la prueba, reagrupándose los ítems en 14 factores (F que representan el 61,3% de la varianza. F1: CCE-TP antisocial (8 ítems, a 0,839; F2: CCE-TP esquizotípico/límite (8 ítems, a 0,846; F3: CCE-TP histriónico/patrón seductor (6 ítems, a 0,833; F4: CCE-TP paranoide (6 ítems, a 0,836; F5: CCE-TP por evitación / autopercepción negativa (5 ítems, a 0,755; F6: CCE-TP por dependencia (5 ítems, a 0,797; F7: CCE-TP histriónico/dependencia emocional (4 ítems, a 0,755; F8: CCETP obsesivo-compulsivo/perfeccionista (4 ítems, a 0,808; F9: CCE-TP por evitación/hipersensible (4 ítems, a 0,766; F10: CCE-TP obsesivo-compulsivo/ crítico frente a los demás (3 ítems, a 0,851; F11: CCE-TP narcisista (4 ítems, a 0,717; F12: CCE-TP pasivo-agresivo / temor a ser dominado (3 ítems, a 0,719; F13: CCE-TP pasivo-agresivo/crítico frente a la autoridad (3 ítems, a 0,685, y F14: CCE-TP esquizoide (2 ítems, a 0,774. El alfa de Cronbach de la prueba fue de 0,931.

Diffuse large B-cell lymphoma with combined TP53 mutation and MIR34A> methylation

DEFF Research Database (Denmark)

Asmar, Fazila; Hother, Christoffer; Kulosman, Gorjan

2014-01-01

and MIR34A methylation ("double hit") and these patients have an exceedingly poor prognosis with a median survival of 9.4 months (Phit") influence on survival. The TP53/MIR34A "double-hit" is an independent...... negative prognostic factor for survival (P=0.0002). In 2 DLBCL-cell lines with both TP53 mutation and promoter methylation of MIR34A, miR34A-5p is upregulated by 5-aza-2'deoxycytidine. Thus, the TP53/MIR34A "double hit" characterizes a very aggressive subgroup of DLBCL, which may be treatable...

Evaluation of FlaB1, FlaB2, FlaB3, and Tp0463 of Treponema pallidum for serodiagnosis of syphilis.

Science.gov (United States)

Jiang, Chuanhao; Xiao, Jinhong; Xie, Yafeng; Xiao, Yongjian; Wang, Chuan; Kuang, Xingxing; Xu, Man; Li, Ranhui; Zeng, Tiebing; Liu, Shuanquan; Yu, Jian; Zhao, Feijun; Wu, Yimou

2016-02-01

Syphilis is a multistage disease caused by the invasive spirochete Treponema pallidum subsp. pallidum, and accurate diagnosis is important for the prevention and treatment of syphilis. Here, to identify appropriate diagnostic antigens for serodiagnosis of syphilis, 6 recombinant proteins were expressed in Escherichia coli and purified, including flagellins (FlaB1 [Tp0868], FlaB2 [Tp0792], and FlaB3 [Tp0870]), Tp0463, Tp0751, and Tp1038. The sensitivities were determined by screening sera from individuals with primary (n=82), secondary (n=115), latent (n=105), and congenital (n=65) syphilis. The specificities were determined by screening sera from uninfected controls (n=30) and potentially cross-reactive infections including Lyme disease (n=30), leptospirosis (n=5), and hepatitis B (n=30). Our data showed that FlaB1, FlaB2, FlaB3, Tp0463, and Tp1038 exhibited higher overall sensitivities and specificities for detecting IgG antibody, with 95.4% and 98.9%, 92.6% and 95.8%, 95.1% and 95.8%, 92.6% and 97.9%, and 95.9% and 98.9%, respectively. In contrast, Tp0751 demonstrated only an overall sensitivity of 39.2%. For comparison, the sensitivity and specificity of Architect Syphilis TP were determined to be 98.1% and 93.7%, respectively. In addition, FlaB1, FlaB2, FlaB3, and Tp0463 demonstrated excellent performance for detecting IgM antibody in primary and congenital syphilis, with sensitivities of 76.8% and 83.1%, 72.0% and 87.7%, 74.4% and 89.2%, and 64.6% and 75.3%, respectively. These results indicate that FlaB1, FlaB2, FlaB3, and Tp0463 could be as novel diagnostic candidates for serodiagnosis of syphilis. Copyright © 2016 Elsevier Inc. All rights reserved.

A meta-analysis of the relationship between FGFR3 and TP53 mutations in bladder cancer.

Science.gov (United States)

Neuzillet, Yann; Paoletti, Xavier; Ouerhani, Slah; Mongiat-Artus, Pierre; Soliman, Hany; de The, Hugues; Sibony, Mathilde; Denoux, Yves; Molinie, Vincent; Herault, Aurélie; Lepage, May-Linda; Maille, Pascale; Renou, Audrey; Vordos, Dimitri; Abbou, Claude-Clément; Bakkar, Ashraf; Asselain, Bernard; Kourda, Nadia; El Gaaied, Amel; Leroy, Karen; Laplanche, Agnès; Benhamou, Simone; Lebret, Thierry; Allory, Yves; Radvanyi, François

2012-01-01

TP53 and FGFR3 mutations are the most common mutations in bladder cancers. FGFR3 mutations are most frequent in low-grade low-stage tumours, whereas TP53 mutations are most frequent in high-grade high-stage tumours. Several studies have reported FGFR3 and TP53 mutations to be mutually exclusive events, whereas others have reported them to be independent. We carried out a meta-analysis of published findings for FGFR3 and TP53 mutations in bladder cancer (535 tumours, 6 publications) and additional unpublished data for 382 tumours. TP53 and FGFR3 mutations were not independent events for all tumours considered together (OR = 0.25 [0.18-0.37], p = 0.0001) or for pT1 tumours alone (OR = 0.47 [0.28-0.79], p = 0.0009). However, if the analysis was restricted to pTa tumours or to muscle-invasive tumours alone, FGFR3 and TP53 mutations were independent events (OR = 0.56 [0.23-1.36] (p = 0.12) and OR = 0.99 [0.37-2.7] (p = 0.35), respectively). After stratification of the tumours by stage and grade, no dependence was detected in the five tumour groups considered (pTaG1 and pTaG2 together, pTaG3, pT1G2, pT1G3, pT2-4). These differences in findings can be attributed to the putative existence of two different pathways of tumour progression in bladder cancer: the CIS pathway, in which FGFR3 mutations are rare, and the Ta pathway, in which FGFR3 mutations are frequent. TP53 mutations occur at the earliest stage of the CIS pathway, whereas they occur would much later in the Ta pathway, at the T1G3 or muscle-invasive stage.

Defects in mitophagy promote redox-driven metabolic syndrome in the absence of TP53INP1.

Science.gov (United States)

Seillier, Marion; Pouyet, Laurent; N'Guessan, Prudence; Nollet, Marie; Capo, Florence; Guillaumond, Fabienne; Peyta, Laure; Dumas, Jean-François; Varrault, Annie; Bertrand, Gyslaine; Bonnafous, Stéphanie; Tran, Albert; Meur, Gargi; Marchetti, Piero; Ravier, Magalie A; Dalle, Stéphane; Gual, Philippe; Muller, Dany; Rutter, Guy A; Servais, Stéphane; Iovanna, Juan L; Carrier, Alice

2015-06-01

The metabolic syndrome covers metabolic abnormalities including obesity and type 2 diabetes (T2D). T2D is characterized by insulin resistance resulting from both environmental and genetic factors. A genome-wide association study (GWAS) published in 2010 identified TP53INP1 as a new T2D susceptibility locus, but a pathological mechanism was not identified. In this work, we show that mice lacking TP53INP1 are prone to redox-driven obesity and insulin resistance. Furthermore, we demonstrate that the reactive oxygen species increase in TP53INP1-deficient cells results from accumulation of defective mitochondria associated with impaired PINK/PARKIN mitophagy. This chronic oxidative stress also favors accumulation of lipid droplets. Taken together, our data provide evidence that the GWAS-identified TP53INP1 gene prevents metabolic syndrome, through a mechanism involving prevention of oxidative stress by mitochondrial homeostasis regulation. In conclusion, this study highlights TP53INP1 as a molecular regulator of redox-driven metabolic syndrome and provides a new preclinical mouse model for metabolic syndrome clinical research. © 2015 The Authors. Published under the terms of the CC BY 4.0 license.

Long term steam oxidation of TP 347H FG in power plants

DEFF Research Database (Denmark)

Hansson, Anette Nørgaard; Korcakova, Leona; Hald, John

2005-01-01

The long term oxidation behaviour of TP 347H FG at ultra supercritical steam conditions was assessed by exposing the steel in test superheater loops in a Danish coal-fired power plant. The steamside oxide layer was investigated with scanning electron microscopy and energy dispersive Xray diffract......The long term oxidation behaviour of TP 347H FG at ultra supercritical steam conditions was assessed by exposing the steel in test superheater loops in a Danish coal-fired power plant. The steamside oxide layer was investigated with scanning electron microscopy and energy dispersive Xray...

TP53 genetic polymorphisms, interactions with lifestyle factors and lung cancer risk: a case control study in a Chinese population

International Nuclear Information System (INIS)

Li, Yanli; Su, Jia; Cai, Lin; Yu, Shunzhang; Zhang, Zuo-Feng; Mu, Lina; Chang, Shen-Chih; Niu, Rungui; Liu, Li; Crabtree-Ide, Christina R; Zhao, Baoxing; Shi, Jianping; Han, Xiaoyou; Li, Jiawei

2013-01-01

A pathway-based genotyping analysis suggested rs2078486 was a novel TP53 SNP, but very few studies replicate this association. TP53 rs1042522 is the most commonly studied SNP, but very few studies examined its potential interaction with environmental factors in relation to lung cancer risk. This study aims to examine associations between two TP53 single-nucleotide polymorphisms (SNPs) (rs2078486, rs1042522), their potential interaction with environmental factors and risk of lung cancer. A case–control study was conducted in Taiyuan, China. Unconditional logistic regression was used to estimate odds ratios (ORs) and 95% confidence intervals (95% CIs). Multiplicative and additive interactions between TP53 SNPs and lifestyle factors were evaluated. Variant TP53 rs2078486 SNP was significantly associated with elevated lung cancer risk among smokers (OR: 1.70, 95% CI: 1.08 - 2.67) and individuals with high indoor air pollution exposure (OR: 1.51, 95% CI: 1.00-2.30). Significant or borderline significant multiplicative and additive interactions were found between TP53 rs2078486 polymorphism with smoking and indoor air pollution exposure. The variant genotype of TP53 SNP rs1042522 significantly increased lung cancer risk in the total population (OR: 1.57, 95% CI: 1.11-2.21), but there was no evidence of heterogeneity among individuals with different lifestyle factors. This study confirmed that TP53 rs2078486 SNP is potentially a novel TP53 SNP that may affect lung cancer risk. Our study also suggested potential synergetic effects of TP53 rs2078486 SNP with smoking and indoor air pollution exposure on lung cancer risk

UARS Particle Environment Monitor (PEM) Level 3TP V001

Data.gov (United States)

National Aeronautics and Space Administration — The Particle Environment Monitor (PEM) Level 3TP data product consists of daily, 65.536 second and 2.048 interval time-ordered, vertical profiles of electron and...

Experimental manipulation of TN:TP ratiossuppress cyanobacterial biovolume and microcystinconcentration in large-scale in situ mesocosms

Science.gov (United States)

Harris, Theodore D.; Wilhelm, Frank M.; Graham, Jennifer L.; Loftin, Keith A.

2014-01-01

A global dataset was compiled to examine relations between the total nitrogen to total phosphorus ratio (TN:TP) and microcystin concentration in lakes and reservoirs. Microcystin concentration decreased as TN:TP ratios increased, suggesting that manipulation of the TN:TP ratio may reduce microcystin concentrations. This relationship was experimentally tested by adding ammonium nitrate to increase the TN:TP ratio in large-scale (70 m3), in situ mesocosms located in a eutrophic reservoir that routinely experiences toxic blooms of cyanobacteria. At a TN:TP ratio >75:1, chlorophytes dominated the phytoplankton community in the mesocosms, while cyanobacterial biovolume was significantly reduced and microcystin was not detected. In contrast, the unmanipulated reservoir was dominated by cyanobacteria, and microcystin was detected. Secchi depths were 1.1 to 1.8 times greater in the mesocosms relative to the reservoir. Cladoceran zooplankton had a larger body size (0.14 mm on average) in the mesocosms compared to conspecifics in the reservoir, which was likely related to the higher quality food. Combined, these empirical and experimental data indicate that although nutrient addition is counterintuitive to current cyanobacteria management practices, increasing the TN:TP ratio by adding nitrogen may be a potential short-term management strategy to reduce cyanobacteria and cyanotoxins when other alternatives (e.g., phosphorus reduction) are not possible. Additional experimental studies with careful controls are needed to define best management practices and identify any potential unintended consequences before nitrogen addition is implemented as a lake and reservoir management practice.

Pemetaan Pusat Pelayanan Terpadu Pemberdayaan Perempuan dan Anak (P2TP2A Provinsi Sumatera Bar

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Hallen Abu Bakar

2017-09-01

Full Text Available The Center of Integrated Service for Women and Children Empowerment (P2TP2A is established by the government based community in order to eliminate the violence towards women and children. It also aims to empower the position of women and children. The purpose of the study is to describe comprehensively the existance of P2TP2A in sub-district, district, city, and province in West Sumatra. Descriptive qualitative research was used where the data taken from questionnaire and in-deepthinterview. The study found that the Center of Integrated Service for Women and Children Empowerment was established by following certain procedure, legality and personnel structure. It also found that the majority of P2TP2A in West Sumatra has insufficient infrastructures. Meanwhile, it is only P2TP2A Limpapeh Rumah Nan Gadang of West Sumatera, and P2TP2A Luhak Nan Tuo Batusangkar has sufficient infrastructures. Many of them were located at the corner of Community Empowerment and Women and Family Planning office (BPMPKB. Every P2TP2A in those district and city have made their programs, but they faced some difficulties in realize them doe to financial problems. The human resources were recruited from SKPD, academician, professional, society figures, LSM who concern on gender meanstreaming. They play the role more on providing the service than giving information and empowerment. The last finding of the study showed that the involvement of local government, Health offices, social services, police, ministry of religion, prosecutors and social services have been effective.

Association of the germline TP53 R337H mutation with breast cancer in southern Brazil

International Nuclear Information System (INIS)

Assumpção, Juliana G; Zeferino, Luiz Carlos; Dufloth, Rozany M; Brandalise, Silvia Regina; Yunes, José Andres; Seidinger, Ana Luíza; Mastellaro, Maria José; Ribeiro, Raul C; Zambetti, Gerard P; Ganti, Ramapriya; Srivastava, Kumar; Shurtleff, Sheila; Pei, Deqing

2008-01-01

The germline TP53-R337H mutation is strongly associated with pediatric adrenocortical tumors (ACT) in southern Brazil; it has low penetrance and limited tissue specificity in most families and therefore is not associated with Li-Fraumeni syndrome. However, other tumor types, mainly breast cancer, have been observed in carriers of several unrelated kindreds, raising the possibility that the R337H mutation may also contribute to breast tumorigenesis in a genetic background-specific context. We conducted a case-control study to determine the prevalence of the R337H mutation by sequencing TP53 exon 10 in 123 women with breast cancer and 223 age- and sex-matched control subjects from southern Brazil. Fisher's test was used to compare the prevalence of the R337H. The R337H mutation was found in three patients but in none of the controls (p = 0.0442). Among the carriers, two had familial history of cancer meeting the Li-Fraumeni-like criteria. Remarkably, tumors in each of these three cases underwent loss of heterozygosity by eliminating the mutant TP53 allele rather than the wild-type allele. Polymorphisms were identified within the TP53 (R72P and Ins16) and MDM2 (SNP309) genes that may further diminish TP53 tumor suppressor activity. These results demonstrate that the R337H mutation can significantly increase the risk of breast cancer in carriers, which likely depends on additional cooperating genetic factors. These findings are also important for understanding how low-penetrant mutant TP53 alleles can differentially influence tumor susceptibility

TP53 supports basal-like differentiation of mammary epithelial cells by preventing translocation of deltaNp63 into nucleoli

Science.gov (United States)

Munne, Pauliina M.; Gu, Yuexi; Tumiati, Manuela; Gao, Ping; Koopal, Sonja; Uusivirta, Sanna; Sawicki, Janet; Wei, Gong-Hong; Kuznetsov, Sergey G.

2014-04-01

Multiple observations suggest a cell type-specific role for TP53 in mammary epithelia. We developed an in vitro assay, in which primary mouse mammary epithelial cells (mMECs) progressed from lumenal to basal-like phenotypes based on expression of Krt18 or ΔNp63, respectively. Such transition was markedly delayed in Trp53-/- mMECs suggesting that Trp53 is required for specification of the basal, but not lumenal cells. Evidence from human basal-like cell lines suggests that TP53 may support the activity of ΔNp63 by preventing its translocation from nucleoplasm into nucleoli. In human lumenal cells, activation of TP53 by inhibiting MDM2 or BRCA1 restored the nucleoplasmic expression of ΔNp63. Trp53-/- mMECs eventually lost epithelial features resulting in upregulation of MDM2 and translocation of ΔNp63 into nucleoli. We propose that TP63 may contribute to TP53-mediated oncogenic transformation of epithelial cells and shed light on tissue- and cell type-specific biases observed for TP53-related cancers.

Microstructure and properties of TP2 copper tube with La microalloying by horizontal continuous casting

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Jin-hu Wu

2018-01-01

Full Text Available The TP2 copper tube was prepared with La microalloying by horizontal continuous casting (HCC. The absorptivity of La and its effects on microstructure, tensile and corrosion properties of HCC TP2 copper tube were studied by means of the inductively coupled plasma optical emission spectrometer (ICP-OES, optical microscope (OM, scanning electron microscope (SEM and potentiodynamic polarization measurements. The results show that the absorptivity of La in the HCC TP2 copper tube is about 15% under antivacuum conditions due to the good chemical activities of La. The impurity elements in copper tube such as O, S, Pb and Si can be significantly reduced, and the average columnar dendrite spacing of the copper tube can also be reduced from 2.21 mm to 0.93 mm by adding La. The ultimate tensile strength and the elongation with and without La addition are almost unchanged. However, the annual corrosion rate of the HCC TP2 copper tube is reduced from 10.18 mm•a-1 to 9.37 mm•a-1 by the purification effect of trace La.

TP53 mutations in myelodysplastic syndrome are strongly correlated with aberrations of chromosome 5, and correlate with adverse prognosis.

Science.gov (United States)

Kulasekararaj, Austin G; Smith, Alexander E; Mian, Syed A; Mohamedali, Azim M; Krishnamurthy, Pramila; Lea, Nicholas C; Gäken, Joop; Pennaneach, Coralie; Ireland, Robin; Czepulkowski, Barbara; Pomplun, Sabine; Marsh, Judith C; Mufti, Ghulam J

2013-03-01

This study aimed to determine the incidence/prognostic impact of TP53 mutation in 318 myelodysplastic syndrome (MDS) patients, and to correlate the changes to cytogenetics, single nucleotide polymorphism array karyotyping and clinical outcome. The median age was 65 years (17-89 years) and median follow-up was 45 months [95% confidence interval (CI) 27-62 months]. TP53 mutations occurred in 30 (9.4%) patients, exclusively in isolated del5q (19%) and complex karyotype (CK) with -5/5q-(72%), correlated with International Prognostic Scoring System intermediate-2/high, TP53 protein expression, higher blast count and leukaemic progression. Patients with mutant TP53 had a paucity of mutations in other genes implicated in myeloid malignancies. Median overall survival of patients with TP53 mutation was shorter than wild-type (9 versus 66 months, P disappearance of the mutant clone or emergence of new clones, suggesting an early occurrence of TP53 mutations. A reduction in mutant clone correlated with response to 5-azacitidine, however clones increased in non-responders and persisted at relapse. The adverse impact of TP53 persists after adjustment for cytogenetic risk and is of practical importance in evaluating prognosis. The relatively common occurrence of these mutations in two different prognostic spectrums of MDS, i.e. isolated 5q- and CK with -5/5q-, possibly implies two different mechanistic roles for TP53 protein. © 2013 Crown copyright. This article is published with the permission of the Controller of HMSO and the Queen's Printer for Scotland.

New recurrent deletions in the PPARgamma and TP53 genes are associated with childhood myelodysplastic syndrome

DEFF Research Database (Denmark)

Silveira, Cássia G T; Oliveira, Fábio M; Valera, Elvis T

2009-01-01

Myelodysplastic syndrome (MDS) is a rare hematological malignancy in children. It was performed FISH analysis in 19 pediatric MDS patients to investigate deletions involving the PPARgamma and TP53 genes. Significant losses in the PPARgamma gene and deletions in the tumor suppressor gene TP53 were...

A meta-analysis of the relationship between FGFR3 and TP53 mutations in bladder cancer.

Directory of Open Access Journals (Sweden)

Yann Neuzillet

Full Text Available TP53 and FGFR3 mutations are the most common mutations in bladder cancers. FGFR3 mutations are most frequent in low-grade low-stage tumours, whereas TP53 mutations are most frequent in high-grade high-stage tumours. Several studies have reported FGFR3 and TP53 mutations to be mutually exclusive events, whereas others have reported them to be independent. We carried out a meta-analysis of published findings for FGFR3 and TP53 mutations in bladder cancer (535 tumours, 6 publications and additional unpublished data for 382 tumours. TP53 and FGFR3 mutations were not independent events for all tumours considered together (OR = 0.25 [0.18-0.37], p = 0.0001 or for pT1 tumours alone (OR = 0.47 [0.28-0.79], p = 0.0009. However, if the analysis was restricted to pTa tumours or to muscle-invasive tumours alone, FGFR3 and TP53 mutations were independent events (OR = 0.56 [0.23-1.36] (p = 0.12 and OR = 0.99 [0.37-2.7] (p = 0.35, respectively. After stratification of the tumours by stage and grade, no dependence was detected in the five tumour groups considered (pTaG1 and pTaG2 together, pTaG3, pT1G2, pT1G3, pT2-4. These differences in findings can be attributed to the putative existence of two different pathways of tumour progression in bladder cancer: the CIS pathway, in which FGFR3 mutations are rare, and the Ta pathway, in which FGFR3 mutations are frequent. TP53 mutations occur at the earliest stage of the CIS pathway, whereas they occur would much later in the Ta pathway, at the T1G3 or muscle-invasive stage.

Synthesis of /sup 75/Se-2-phenyl-1,2-benzisoselenazol-3(2H)-one (PZ 51; Ebselen). A novel biologically active organo-selenium compound

Energy Technology Data Exchange (ETDEWEB)

Cantineau, R.; Tihange, G.; Plenevaux, A.; Christiaens, L.; Guillaume, M.; Welter, A.; Dereu, N.

1986-01-01

The preparation of /sup 75/Se-ebselen (/sup 75/Se-PZ 51) in a high radiochemical yield (approx. 40%) and with a specific activity of 240 mCi/mM (8.9 GBq/mM) is described. It entails a very simple, fast and one-pot procedure starting from elemental /sup 75/Se-selenium. /sup 75/Se-diselenosalicylic acid is initially prepared as the key intermediate which is transformed into a corresponding dichloride before reacting with aniline to yield the desired /sup 75/Se-ebselen. Identity and purity of the labelled compound were controlled by comparison in TLC, HPLC and MS with an authentic sample.

Influencia de la densidad y del confinamiento con geotextil en columnas de grava

OpenAIRE

Miranda Manzanares, Marina

2014-01-01

RESUMEN: El tratamiento con columnas de grava es uno de los métodos de mejora del terreno empleados habitualmente para la cimentación de terraplenes o estructuras sobre suelos blandos. El estudio realizado en esta Tesis Doctoral se centra en analizar: - La influencia de la densidad de la grava de la columna - El empleo de columnas ensacadas con geotextil. El análisis de la influencia de la densidad de la grava se ha realizado mediante ensayos realizados en laboratorio en modelo reducido con d...

Influencia de la densidad y del confinamiento con geotextil en columnas de grava

OpenAIRE

Miranda Manzanares, Marina

2014-01-01

El tratamiento con columnas de grava es uno de los métodos de mejora del terreno empleados habitualmente para la cimentación de terraplenes o estructuras sobre suelos blandos. El estudio realizado en esta Tesis Doctoral se centra en analizar: - La influencia de la densidad de la grava de la columna - El empleo de columnas ensacadas con geotextil. El análisis de la influencia de la densidad de la grava se ha realizado mediante ensayos realizados en laboratorio en modelo reducido con dos densid...

Association of the germline TP53 R337H mutation with breast cancer in southern Brazil

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Srivastava Kumar

2008-12-01

Full Text Available Abstract Background The germline TP53-R337H mutation is strongly associated with pediatric adrenocortical tumors (ACT in southern Brazil; it has low penetrance and limited tissue specificity in most families and therefore is not associated with Li-Fraumeni syndrome. However, other tumor types, mainly breast cancer, have been observed in carriers of several unrelated kindreds, raising the possibility that the R337H mutation may also contribute to breast tumorigenesis in a genetic background-specific context. Methods We conducted a case-control study to determine the prevalence of the R337H mutation by sequencing TP53 exon 10 in 123 women with breast cancer and 223 age- and sex-matched control subjects from southern Brazil. Fisher's test was used to compare the prevalence of the R337H. Results The R337H mutation was found in three patients but in none of the controls (p = 0.0442. Among the carriers, two had familial history of cancer meeting the Li-Fraumeni-like criteria. Remarkably, tumors in each of these three cases underwent loss of heterozygosity by eliminating the mutant TP53 allele rather than the wild-type allele. Polymorphisms were identified within the TP53 (R72P and Ins16 and MDM2 (SNP309 genes that may further diminish TP53 tumor suppressor activity. Conclusion These results demonstrate that the R337H mutation can significantly increase the risk of breast cancer in carriers, which likely depends on additional cooperating genetic factors. These findings are also important for understanding how low-penetrant mutant TP53 alleles can differentially influence tumor susceptibility.

A novel ELISA using a recombinant outer membrane protein, rTp0663, as the antigen for serological diagnosis of syphilis.

Science.gov (United States)

Xu, Man; Xie, Yafeng; Jiang, Chuanhao; Xiao, Yongjian; Kuang, Xingxing; Zhao, Feijun; Zeng, Tiebing; Liu, Shuangquan; Liang, Mingxing; Li, Li; Wang, Chuan; Wu, Yimou

2016-02-01

The lack of Treponema pallidum-specific antigens with highly accurate diagnosis makes the diagnosis of syphilis challenging. A soluble recombinant version of a new diagnostic protein Tp0663 has been produced. The serodiagnostic potential of this protein was assessed by screening 3326 serum samples simultaneously evaluated by rapid plasma reagin and T. pallidum particle agglutination tests. Kappa (κ) coefficients were used to compare the concordance between clinical diagnosis and the Tp0663-based ELISA or the ARCHITECT Syphilis TP chemiluminescent immunoassay (Abbott GmbH and Co. KG). Using the results of clinical diagnosis as the gold standard, the sensitivity and specificity of Tp0663 were found to be 98.83% (95% confidence interval (CI) 96.61-99.60%) and 100% (95% CI 99.88-100%), respectively. In comparison, the ARCHITECT Syphilis TP assay was found to have a lower sensitivity (97.27%, 95% CI 94.46-98.67%) and specificity (99.61%, 95% CI 99.32-99.78%). In particular, the ARCHITECT Syphilis TP exhibited a false-positive rate of 0.39%. Moreover, the ELISA was in perfect agreement with the gold standard, with a κ value of 0.99, comparable to that of ARCHITECT Syphilis TP (0.96). These results identified Tp0663 as a novel serodiagnostic candidate with great potential for developing novel tests for the diagnosis of syphilis. Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.

TP53 p.R337H is a conditional cancer-predisposing mutation: further evidence from a homozygous patient

International Nuclear Information System (INIS)

Giacomazzi, Juliana; Hainaut, Pierre; Ashton-Prolla, Patricia; Selistre, Simone; Duarte, Juliana; Ribeiro, Jorge Pinto; Vieira, Paulo JC; Souza Macedo, Gabriel de; Rossi, Cristina; Czepielewski, Mauro; Netto, Cristina Brinkmann Oliveira

2013-01-01

Adrenocortical carcinomas (ACCs) are among the most common childhood cancers occurring in infants affected with the Li-Fraumeni and Li- Fraumeni-like (LFS/LFL) syndromes, which are caused by dominant germline mutations in the TP53 gene. In Brazil, a particular mutation, occurring in the tetramerisation domain of the gene, p.R337H, is exceedingly common due to a founder effect and is strongly associated with ACC. In this report, we describe the phenotype and long-term clinical follow-up of a female child diagnosed with ACC and homozygous for the TP53 p.R337H founder mutation. At age 11 months, the patient was diagnosed with a virilising anaplastic adrenal cortical tumour, which was completely excised without disturbing the adrenal capsule. Family history was consistent with an LFL tumour pattern, and genotyping identified the TP53 p.R337H mutation in both alleles in genomic DNA from lymphocytes and fibroblasts. Haplotype analysis confirmed the occurrence of the mutation in the same founder haplotype previously described in other Brazilian patients. No other germline or somatic TP53 mutations or rearrangements were identified. At age 9 years, the child was asymptomatic and had no evidence of endocrine derangements. Full body and brain magnetic resonance imaging (MRI) failed to detect any suspicious proliferative lesions, and cardiopulmonary exercise testing results were within the normal reference for the child’s age, ruling out a major exercise capacity deficiency. This is the first clinical and aerobic functional capacity documentation of a patient who carries two mutant TP53 alleles and no wild-type allele. Our results support the hypothesis that TP53 p.R337H, the most common TP53 mutation ever described in any population, is a conditional mutant. Furthermore, our observations over a long period of clinical follow-up suggest that TP53 p.R337H homozygotes do not have a more severe disease phenotype than do heterozygote carriers of the same mutation. Patients with

Mutational analysis of the activator of late transcription, Alt , in the lactococcal bacteriophage TP901-1

DEFF Research Database (Denmark)

Pedersen, Margit; Hammer, Karin

2007-01-01

An activator protein, Alt, synthesized during the early state of lytic infection is required for transcription of the late operon in the lactococcal phage TP901-1. In order to identify amino acid residues in the Alt protein required for activation of the TP901-1 late promoter, Plate, hydroxylamine...

Clinical Impact of TP53 Gene Mutations in Diffuse Large B-Cell Lymphoma (DLBCL)

DEFF Research Database (Denmark)

Young, Ken H; Patten, Nancy; Truong, Sim

2009-01-01

Mutations of the TP53 tumor suppressor gene are associated with a poor clinical outcome in DLBCL patients treated with CHOP. The impact of TP53 mutations on clinical outcome of DLBCL patients treated with Rituxan-CHOP has not been comprehensively analyzed. The purpose of this study was to analyze...

License - TP Atlas | LSDB Archive [Life Science Database Archive metadata

Lifescience Database Archive (English)

Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data ... might be changed without notice. About This Database Database Description Download License Update History of This Database Site Policy | Contact Us License - TP Atlas | LSDB Archive ...

Effect of reciprocating agitation thermal processing (RA-TP) on quality of canned tomato (Solanum lycopersicum) puree.

Science.gov (United States)

Pratap Singh, Anubhav; Singh, Anika; Ramaswamy, Hosahalli S

2017-06-01

Reciprocating agitation thermal processing (RA-TP) is a recent innovation in the field of canning for obtaining high-quality canned food. The objective of this study was to compare RA-TP processing with conventional non-agitated (still) processing with respect to the impact on quality (color, antioxidant capacity, total phenols, carotenoid and lycopene contents) of canned tomato (Solanum lycopersicum) puree. Owing to a 63-81% reduction in process times as compared with still processing, tomato puree with a brighter red color (closer to fresh) was obtained during RA-TP. At 3 Hz reciprocation frequency, the loss of antioxidant, lycopene and carotenoid contents could be reduced to 34, 8 and 8% respectively as compared with 96, 41 and 52% respectively during still processing. In fact, the phenolic content for RA-TP at 3 Hz was 5% higher than in fresh puree. Quality retention generally increased with an increase in frequency, although the differences were less significant at higher reciprocation frequencies (between 2 and 3 Hz). Research findings indicate that RA-TP can be effective to obtain thermally processed foods with high-quality attribute retention. It can also be concluded that a very high reciprocation frequency (>3 Hz) is not necessarily needed and significant quality improvement can be obtained at lower frequencies (∼2 Hz). © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

FOXP1 and TP63 involvement in the progression of myelodysplastic syndrome with 5q- and additional cytogenetic abnormalities

International Nuclear Information System (INIS)

L’Abbate, Alberto; Tagliafico, Enrico; Minoia, Carla; De Tullio, Giacoma; Guarini, Attilio; Testoni, Nicoletta; Agostinelli, Claudio; Storlazzi, Clelia Tiziana; Lo Cunsolo, Crocifissa; Macrì, Ettore; Iuzzolino, Paolo; Mecucci, Cristina; Doglioni, Claudio; Coco, Michelina; Muscarella, Lucia Anna; Salati, Simona

2014-01-01

The progression of low-risk del(5q) myelodysplastic syndrome to acute myeloid leukemia is increased when associated with mutations of TP53, or with additional chromosomal abnormalities. However, to date the prognostic impact and molecular consequences of these rearrangements were poorly investigated. Single additional alterations to del(5q) by balanced chromosome rearrangements were rarely found in myelodysplasia. In particular, balanced alterations involving TP63 and FOXP1 genes were never reported in the literature. Here we report on a 79-year woman with an aggressive form of myelodysplastic syndrome with del(5q), no TP53 mutation, and a novel complex rearrangement of chromosome 3 in bone marrow cells. Our results revealed that the FOXP1 and TP63 genes were both relocated along chromosome 3. Strikingly, immunohistochemistry analysis showed altered protein levels, disclosing that this rearrangement triggered the expression of FOXP1 and TP63 genes. FOXP1 was also found activated in other patients with myelodysplasia and acute myeloid leukemia, showing that it is an important, recurrent event. We document an apparent role of FOXP1 and TP63, up to now poorly documented, in the progression of MDS in our patient who is lacking mutations in the TP53 tumor suppressor gene normally associated with poor outcome in myelodysplastic syndrome with 5q-. Finally, our results may suggest a possible broader role of FOXP1 in the pathogenesis and progression of myelodysplasia and acute myeloid leukemia

Extremely high Tp53 mutation load in esophageal squamous cell carcinoma in Golestan Province, Iran.

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Behnoush Abedi-Ardekani

Full Text Available BACKGROUND: Golestan Province in northeastern Iran has one of the highest incidences of esophageal squamous cell carcinoma (ESCC in the world with rates over 50 per 100,000 person-years in both sexes. We have analyzed TP53 mutation patterns in tumors from this high-risk geographic area in search of clues to the mutagenic processes involved in causing ESCC. METHODOLOGY/PRINCIPAL FINDINGS: Biopsies of 119 confirmed ESCC tumor tissue from subjects enrolled in a case-control study conducted in Golestan Province were analyzed by direct sequencing of TP53 exons 2 through 11. Immunohistochemical staining for p53 was carried out using two monoclonal antibodies, DO7 and 1801. A total of 120 TP53 mutations were detected in 107/119 cases (89.9%, including 11 patients with double or triple mutations. The mutation pattern was heterogeneous with infrequent mutations at common TP53 "hotspots" but frequent transversions potentially attributable to environmental carcinogens forming bulky DNA adducts, including 40% at bases known as site of mutagenesis by polycyclic aromatic hydrocarbons (PAHs. Mutations showed different patterns according to the reported temperature of tea consumption, but no variation was observed in relation to ethnicity, tobacco or opium use, and alcoholic beverage consumption or urban versus rural residence. CONCLUSION/SIGNIFICANCE: ESCC tumors in people from Golestan Province show the highest rate of TP53 mutations ever reported in any cancer anywhere. The heterogeneous mutation pattern is highly suggestive of a causative role for multiple environmental carcinogens, including PAHs. The temperature and composition of tea may also influence mutagenesis.

ESTADO DEL ARTE MPLS-TP CONMUTACIÓN MULTIPROTOCOLO MEDIANTE ETIQUETAS - PERFIL DE TRANSPORTE

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Lois Albert Espinel García

2013-09-01

Full Text Available La creciente demanda en servicios de telecomunicaciones genera la necesidad de desarrollar diferentes modelos de red. En el año 2008, la UIT-T une sinergias con la IETF para desarrollar un protocolo llamado MPLS-TP cuya arquitectura representa el resultado la agregación de la eficiencia en el transporte de paquetes de IP/MPLS con la fiabilidad en el transporte de SONET/SDH. Este trabajo presenta un estado del arte de MPLS-TP donde se plasman los estudios actuales y avances relacionados con ésta tecnología: modelos de arquitectura óptica, modelos de protección en OAM. Además se presenta la estructura, funcionamiento y características que hacen de MPLS-TP una opción para las redes de transporte de siguiente generación. El material de apoyo para el desarrollo de este artículo ha sido obtenido de la base documental de recursos electrónicos con contenido científico de apoyo a la investigación de la Universidad Distrital Francisco José de Caldas.

Stress Rupture and Precipitation Behavior of TP310HCbN(HR3C for Supercritical Boilers

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FANG Xu-dong

2017-06-01

Full Text Available Using persistent experiment method, combined with Gleeble,hardness analysis, SEM, EDS, TEM and other analytical methods, the hot deformation, stress rupture and precipitation behavior of TP310HCbN heat resistance steel was analysed at 650℃ and 700℃, the results show that:the change of the hardness is not obviously under two different stress rupture temperature; with stress rupture time prolongs, TP310HCbN heat-resistant steel intragranular precipitates from granular into rod-shaped, and presence of wormlike NbCrN rich precipitates and dislocation interactions; Taiyuan Iron and Steel production of TP310HCbN heat-resistant steel at 650/700℃-100000h extrapolation lasting strength meet the standard requirements.

TP Atlas: integration and dissemination of advances in Targeted Proteins Research Program (TPRP)-structural biology project phase II in Japan.

Science.gov (United States)

Iwayanagi, Takao; Miyamoto, Sei; Konno, Takeshi; Mizutani, Hisashi; Hirai, Tomohiro; Shigemoto, Yasumasa; Gojobori, Takashi; Sugawara, Hideaki

2012-09-01

The Targeted Proteins Research Program (TPRP) promoted by the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan is the phase II of structural biology project (2007-2011) following the Protein 3000 Project (2002-2006) in Japan. While the phase I Protein 3000 Project put partial emphasis on the construction and maintenance of pipelines for structural analyses, the TPRP is dedicated to revealing the structures and functions of the targeted proteins that have great importance in both basic research and industrial applications. To pursue this objective, 35 Targeted Proteins (TP) Projects selected in the three areas of fundamental biology, medicine and pharmacology, and food and environment are tightly collaborated with 10 Advanced Technology (AT) Projects in the four fields of protein production, structural analyses, chemical library and screening, and information platform. Here, the outlines and achievements of the 35 TP Projects are summarized in the system named TP Atlas. Progress in the diversified areas is described in the modules of Graphical Summary, General Summary, Tabular Summary, and Structure Gallery of the TP Atlas in the standard and unified format. Advances in TP Projects owing to novel technologies stemmed from AT Projects and collaborative research among TP Projects are illustrated as a hallmark of the Program. The TP Atlas can be accessed at http://net.genes.nig.ac.jp/tpatlas/index_e.html .

Contribution of germline TP53 variants and assessment of HER-2 status among young breast cancer patients in Malaysia

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Shao Yan Lau

2017-12-01

Full Text Available Background: Li-Fraumeni Syndrome (LFS is caused by a mutation in the TP53 tumour suppressor gene. This rare hereditary condition predisposes individuals to an increased risk of cancers including breast cancer in women at a relatively young age, which accounts for nearly 25%–30% of all LFS‑associated cancers. Studies have shown that breast tumours in women with a germline TP53 deleterious variants are associated with a human epidermal growth factor receptor 2 (HER2-positive phenotype. Taken together, this study aimed to investigate the contribution of germline TP53 variants and its association with tumour HER-2 status in a cohort of young women with breast cancer. Methods: From 2002 to 2017, 4048 women with breast cancer treated at University Malaya Medical Centre or Sime Darby Medical Centre participated in the Malaysian Breast Cancer Genetics Study. Of which, 87 patients were diagnosed before 30 years of age. All patients were analysed for germline TP53 single nucleotide variants, small insertions or deletions by amplicon‑based targeted sequencing and validated by Sanger sequencing. DNA from patients who tested negative for sequencing were subsequently evaluated for the presence of TP53 exon deletions or duplications by multiplex ligation‑dependent probe amplification. HER-2 status of breast tumours was defined by immunohistochemistry, fluorescence in situ hybridisation and/or silver in situ hybridisation. Results: 5 distinct TP53 variants were detected in 5 individuals. 3 out of 5 TP53 variants were classified as frameshift mutations, one nonsense mutation and one in-frame duplication. Variants in other genes were detected in 17 individuals. No large genomic rearrangements were detected in the remaining 65 sequencing-negative patients. The assessment of HER-2 status will be presented. Conclusions: Our results suggest that alterations in TP53 gene were identified in approximately 5.7% (5/87 of this cohort of young women with breast

Mutations and polymorphisms in TP53 gene--an overview on the role in colorectal cancer

Czech Academy of Sciences Publication Activity Database

Naccarati, Alessio; Poláková, Veronika; Pardini, Barbara; Vodičková, Ludmila; Hemminki, K.; Kumar, R.; Vodička, Pavel (ed.)

2012-01-01

Roč. 27, č. 2 (2012), s. 211-218 ISSN 0267-8357 R&D Projects: GA ČR GP305/09/P194; GA ČR GAP304/10/1286 Grant - others:GA UK(CZ) GA96908/B/2008 Institutional research plan: CEZ:AV0Z50390512 Keywords : TP53 mutations * TP53 polymorphisms * cancer risk Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.500, year: 2012

Phylogenetic analysis of human Tp53 gene using computational ...

African Journals Online (AJOL)

The TP53 gene encoding p53 protein is involved in regulating a series of pathways. New discoveries about the function and control of p53 are still in progress and it is hoped to develop better therapeutics and diagnostics by exploiting this system. Evolutionary studies are of prime importance in the field of biological ...

Rhabdomyosarcoma-associated renal cell carcinoma: a link with constitutional Tp53 mutation.

LENUS (Irish Health Repository)

Curry, Sarah

2012-02-01

The 2004 World Health Organization classification includes the new entity "neuroblastoma-associated renal cell carcinoma." The pathogenetic link between these entities is unknown as yet. The patient reported herein developed renal cell carcinoma after anaplastic embryonal rhabdomyosarcoma, a previously unknown association. The 2nd malignancy developed very soon after the 1st one, prompting concern for inherent cancer predisposition rather than a therapy-induced 2nd malignancy. A variety of features raised suspicion for Tp53 mutation, and indeed a pathogenic germline Tp53 mutation was identified in this child, despite a negative family history for Li-Fraumeni syndrome. Consideration of underlying predisposition is advocated in the context of rapid evolution of 2nd childhood malignancy.

TP53 mutation and human papilloma virus status of oral squamous cell carcinomas in young adult patients

NARCIS (Netherlands)

Braakhuis, B.J.M.; Rietbergen, M.M.; Buijze, M.; Snijders, P.J.F.; Bloemena, E.; Brakenhoff, R.H.; Leemans, C.R.

2014-01-01

Objective Little is known about the molecular carcinogenesis of oral squamous cell carcinoma (OSCC) in young adult patients. The aim of this study was to investigate the detailed TP53 mutation and human papilloma virus (HPV) status of OSCC in patients, younger than 45 years. Methods TP53 mutations

Prognostic value of TP53 transcriptional activity on p21 and bax in patients with esophageal squamous cell carcinomas treated by definitive chemoradiotherapy

International Nuclear Information System (INIS)

Michel, Pierre; Magois, Karine; Robert, Valerie; Chiron, Anne; Lepessot, Florence; Bodenant, Corinne; Roque, Isabelle; Seng, Sok H.; Frebourg, Thierry; Paillot, Bernard

2002-01-01

Purpose: The aim of this study was to evaluate biologic factors on survival and clinical response after definitive concomitant chemoradiotherapy (CRT) in patients with esophageal squamous cell carcinoma (ESCC). Methods and Materials: TP53 protein hyperexpression (immunochemistry [IHC]) and functional assay (FA) of TP53, measuring the ability of TP53 to transactivate p21 and bax reporter systems, were performed in patients with ESCC treated by CRT. The impact of parameters studied on survival and clinical response to CRT was assessed. Results: Thirty-eight patients with ESCC were included. TP53 alterations were detected in 84.2% of cases with FA. All TP53 mutations abolished the transactivation of p21 and bax reporter systems. After CRT, complete response rate was 55.3%. The median survival of the population was 17.5 months. Serum albumin (p=0.002), weight loss <10% (p=0.005), and response to treatment (p<0.001) were significantly linked with survival. TP53 alteration in FA was not significantly predictive of response to CRT (p=0.132) nor survival (p=0.154). Conclusions: Our results suggest that wild-type TP53 in ESCC could be associated with good response to definitive CRT. However, the small rate of ESCC with wild-type TP53 suggests that systematic determination of TP53 status is not appropriate for the management of the ESCC population

The Relationship between TP53 Gene Status and Carboxylesterase 2 Expression in Human Colorectal Cancer

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Momoko Ishimine

2018-01-01

Full Text Available Irinotecan (CPT-11 is an anticancer prodrug that is activated by the carboxylesterase CES2 and has been approved for the treatment of many types of solid tumors, including colorectal cancer. Recent studies with cell lines show that CES2 expression is regulated by the tumor suppressor protein p53. However, clinical evidence for this regulatory mechanism in cancer is lacking. In this study, we examined the relationship between TP53 gene status and CES2 expression in human colorectal cancer. Most colorectal cancer specimens (70%; 26 of 37 showed lower CES2 mRNA levels (≥1.5-fold lower than the adjacent normal tissue, and only 30% (12 of 37 showed similar (<1.5-fold lower or higher CES2 mRNA levels. However, TP53 gene sequencing revealed no relationship between CES2 downregulation and TP53 mutational status. Moreover, while colorectal cancer cells expressing wild-type p53 exhibited p53-dependent upregulation of CES2, PRIMA-1MET, a drug that restores the transcriptional activity of mutant p53, failed to upregulate CES2 expression in cells with TP53 missense mutations. These results, taken together, suggest that CES2 mRNA expression is decreased in human colorectal cancer independently of p53.

Música y cerebro: influencia del arte musical en la biología humana

OpenAIRE

Siesto Sánchez, Violeta

2017-01-01

Trabajo de fin de grado. Grado en Biología. Curso académico 2016-2017 El trabajo analiza la posible influencia de la música en el desarrollo de las habilidades humanas. Se analiza la anatomía humana relacionada en el proceso, desde el sistema auditivo hasta la corteza cerebral. La segunda parte del estudio lo pone en relación con el proceso de percepción y se refiere a la relaciónd el hombre con la música a lo largo de la historia, con particular atención a la influencia que pueda haber t...

TP53 alterations in Wilms tumour represent progression events with strong intratumour heterogeneity that are closely linked but not limited to anaplasia

OpenAIRE

Wegert, Jenny; Vokuh, Christian; Ziegler, Barbara; Ernestus, Karen; Leuschner, Ivo; Furtwängler, Rhoikos; Graf, Norbert; Gessler, Manfred

2018-01-01

TP53 mutations have been associated with anaplasia in Wilms tumour, which conveys a high risk for relapse and fatal outcome. Nevertheless, TP53 alterations have been reported in no more than 60% of anaplastic tumours, and recent data have suggested their presence in tumours that do not fulfil the criteria for anaplasia, questioning the clinical utility of TP53 analysis. Therefore, we characterized the TP53 status in 84 fatal cases of Wilms tumour, irrespective of histological subtype. We iden...

TP53 alterations in Wilms tumour represent progression events with strong intratumour heterogeneity that are closely linked but not limited to anaplasia

OpenAIRE

Wegert, Jenny; Vokuhl, Christian; Ziegler, Barbara; Ernestus, Karen; Leuschner, Ivo; Furtwängler, Rhoikos; Graf, Norbert; Gessler, Manfred

2017-01-01

Abstract TP53 mutations have been associated with anaplasia in Wilms tumour, which conveys a high risk for relapse and fatal outcome. Nevertheless, TP53 alterations have been reported in no more than 60% of anaplastic tumours, and recent data have suggested their presence in tumours that do not fulfil the criteria for anaplasia, questioning the clinical utility of TP53 analysis. Therefore, we characterized the TP53 status in 84 fatal cases of Wilms tumour, irrespective of histological subtype...

TP53 genetic polymorphisms, interactions with lifestyle factors and lung cancer risk: a case control study in a Chinese population

OpenAIRE

Li, Yanli; Chang, Shen-Chih; Niu, Rungui; Liu, Li; Crabtree-Ide, Christina R; Zhao, Baoxing; Shi, Jianping; Han, Xiaoyou; Li, Jiawei; Su, Jia; Cai, Lin; Yu, Shunzhang; Zhang, Zuo-Feng; Mu, Lina

2013-01-01

Abstract Background A pathway-based genotyping analysis suggested rs2078486 was a novel TP53 SNP, but very few studies replicate this association. TP53 rs1042522 is the most commonly studied SNP, but very few studies examined its potential interaction with environmental factors in relation to lung cancer risk. This study aims to examine associations between two TP53 single-nucleotide polymorphisms (SNPs) (rs2078486, rs1042522), their potential interac...

INFLUENCIA DEL TEMPO DE LA MÚSICA EN LAS EMOCIONES

Directory of Open Access Journals (Sweden)

Jonathan Azael Caballero-Meneses

2010-01-01

Full Text Available Se sabe que la música puede influir en dos componentes de lasemociones: el arousal y la valencia. En particular, se ha reportadoque el tempo o rapidez de la música está directamenterelacionado con el arousal, aunque su relación con la valenciao medida de afecto no es aún clara. En este trabajo se analizóla influencia del tempo en ambos componentes mediante laexposición de 24 estudiantes de licenciatura a treinta extractosmusicales de distinto tempo. Además se evaluó la posiblemodulación de la relación tempo-valencia por el rasgo de personalidadextroversión/introversión. Los resultados confirmaronla influencia del tempo sobre el arousal, pero no sobrela valencia, así como una posible mediación de la dimensiónde personalidad introversión-extroversión sobre la valencia.

Inactivation of human osteosarcoma cells in vitro by 211At-TP-3 monoclonal antibody: Comparison with astatine-211 and external-beam X rays

International Nuclear Information System (INIS)

Larsen, R.H.; Bruland, O.S.; Hoff, P.; Alstad, J.; Lindmo, T.; Rofstad, E.K.

1994-01-01

The potential usefulness of α-particle radioimmunotherapy in the treatment of osteosarcoma was studied in vitro by using the monoclonal antibody TP-3 and cells of three human osteosarcoma cell lines (OHS, SAOS and KPDX) differing in antigen expression. Cell survival curves were established after treatment with (a) 211 At-TP-3 of different specific activities, (b) 211 At-labeled bovine serum albumin (BSA), (c) free 211 At and (d) external-beam X rays. The three osteosarcoma cell lines showed similar survival curves, whether treated with external-beam X rays, 211 At-BSA or free 211 At. The D o 's were lower for free 211 At than for 211 At-BSA. The survival curves for 211 At-TP-3 treatment, on the other hand, differed significantly among the cell lines, suggesting that sensitivity to 211 At-TP-3 treatment was governed by cellular properties other than sensitivity to external-beam X rays. The cellular property most important for sensitivity to 211 At-TP-3 treatment was the antigen expression. Cell inactivation after 211 At-TP-3 treatment increased substantially with increasing specific activity of the 211 At-TP-3. At high specific activities, the cytotoxic effect of 211 At-TP-3 was significantly higher than that of 211 At-BSA. In conclusion, 211 At-TP-3 has the potential to give clinically favorable therapeutic ratios in the treatment of osteosarcoma. 39 refs., 5 figs., 2 tabs

A NEW GENERATION OF PARSEC-COLIBRI STELLAR ISOCHRONES INCLUDING THE TP-AGB PHASE

International Nuclear Information System (INIS)

Marigo, Paola; Aringer, Bernhard; Chen, Yang; Dussin, Marco; Nanni, Ambra; Pastorelli, Giada; Rodrigues, Thaíse S.; Trabucchi, Michele; Bladh, Sara; Montalbán, Josefina; Girardi, Léo; Bressan, Alessandro; Rosenfield, Philip; Dalcanton, Julianne; Groenewegen, Martin A. T.; Wood, Peter R.

2017-01-01

We introduce a new generation of PARSEC–COLIBRI stellar isochrones that includes a detailed treatment of the thermally pulsing asymptotic giant branch (TP-AGB) phase, covering a wide range of initial metallicities (0.0001 < Z i < 0.06). Compared to previous releases, the main novelties and improvements are use of new TP-AGB tracks and related atmosphere models and spectra for M and C-type stars; inclusion of the surface H+He+CNO abundances in the isochrone tables, accounting for the effects of diffusion, dredge-up episodes and hot-bottom burning; inclusion of complete thermal pulse cycles, with a complete description of the in-cycle changes in the stellar parameters; new pulsation models to describe the long-period variability in the fundamental and first-overtone modes; and new dust models that follow the growth of the grains during the AGB evolution, in combination with radiative transfer calculations for the reprocessing of the photospheric emission. Overall, these improvements are expected to lead to a more consistent and detailed description of properties of TP-AGB stars expected in resolved stellar populations, especially in regard to their mean photometric properties from optical to mid-infrared wavelengths. We illustrate the expected numbers of TP-AGB stars of different types in stellar populations covering a wide range of ages and initial metallicities, providing further details on the “C-star island” that appears at intermediate values of age and metallicity, and about the AGB-boosting effect that occurs at ages close to 1.6-Gyr for populations of all metallicities. The isochrones are available through a new dedicated web server.

A NEW GENERATION OF PARSEC-COLIBRI STELLAR ISOCHRONES INCLUDING THE TP-AGB PHASE

Energy Technology Data Exchange (ETDEWEB)

Marigo, Paola; Aringer, Bernhard; Chen, Yang; Dussin, Marco; Nanni, Ambra; Pastorelli, Giada; Rodrigues, Thaíse S.; Trabucchi, Michele; Bladh, Sara; Montalbán, Josefina [Dipartimento di Fisica e Astronomia Galileo Galilei, Università di Padova, Vicolo dell’Osservatorio 3, I-35122 Padova (Italy); Girardi, Léo [Osservatorio Astronomico di Padova—INAF, Vicolo dell’Osservatorio 5, I-35122 Padova (Italy); Bressan, Alessandro [SISSA, via Bonomea 365, I-34136 Trieste (Italy); Rosenfield, Philip [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States); Dalcanton, Julianne [Department of Astronomy, University of Washington, Box 351580, Seattle, WA 98195 (United States); Groenewegen, Martin A. T. [Koninklijke Sterrenwacht van België, Ringlaan 3, B-1180 Brussels (Belgium); Wood, Peter R. [Research School of Astronomy and Astrophysics, Australian National University, Cotter Road, Weston Creek, ACT 2611 (Australia)

2017-01-20

We introduce a new generation of PARSEC–COLIBRI stellar isochrones that includes a detailed treatment of the thermally pulsing asymptotic giant branch (TP-AGB) phase, covering a wide range of initial metallicities (0.0001 < Z {sub i} < 0.06). Compared to previous releases, the main novelties and improvements are use of new TP-AGB tracks and related atmosphere models and spectra for M and C-type stars; inclusion of the surface H+He+CNO abundances in the isochrone tables, accounting for the effects of diffusion, dredge-up episodes and hot-bottom burning; inclusion of complete thermal pulse cycles, with a complete description of the in-cycle changes in the stellar parameters; new pulsation models to describe the long-period variability in the fundamental and first-overtone modes; and new dust models that follow the growth of the grains during the AGB evolution, in combination with radiative transfer calculations for the reprocessing of the photospheric emission. Overall, these improvements are expected to lead to a more consistent and detailed description of properties of TP-AGB stars expected in resolved stellar populations, especially in regard to their mean photometric properties from optical to mid-infrared wavelengths. We illustrate the expected numbers of TP-AGB stars of different types in stellar populations covering a wide range of ages and initial metallicities, providing further details on the “C-star island” that appears at intermediate values of age and metallicity, and about the AGB-boosting effect that occurs at ages close to 1.6-Gyr for populations of all metallicities. The isochrones are available through a new dedicated web server.

TP53 codon 72 polymorphism as a risk factor for cardiovascular disease in a Brazilian population

Directory of Open Access Journals (Sweden)

M.A.C. Smith

2007-11-01

Full Text Available TP53, a tumor suppressor gene, has a critical role in cell cycle, apoptosis and cell senescence and participates in many crucial physiological and pathological processes. Identification of TP53 polymorphism in older people and age-related diseases may provide an understanding of its physiology and pathophysiological role as well as risk factors for complex diseases. TP53 codon 72 (TP53:72 polymorphism was investigated in 383 individuals aged 66 to 97 years in a cohort from a Brazilian Elderly Longitudinal Study. We investigated allele frequency, genotype distribution and allele association with morbidities such as cardiovascular disease, type II diabetes, obesity, neoplasia, low cognitive level (dementia, and depression. We also determined the association of this polymorphism with serum lipid fractions and urea, creatinine, albumin, fasting glucose, and glycated hemoglobin levels. DNA was isolated from blood cells, amplified by PCR using sense 5'-TTGCCGTCCCAAGCAATGGATGA-3' and antisense 5'-TCTGGGAAGGGACAGAAGATGAC-3' primers and digested with the BstUI enzyme. This polymorphism is within exon 4 at nucleotide residue 347. Descriptive statistics, logistic regression analysis and Student t-test using the multiple comparison test were used. Allele frequencies, R (Arg = 0.69 and P (Pro = 0.31, were similar to other populations. Genotype distributions were within Hardy-Weinberg equilibrium. This polymorphism did not show significant association with any age-related disease or serum variables. However, R allele carriers showed lower HDL levels and a higher frequency of cardiovascular disease than P allele subjects. These findings may help to elucidate the physiopathological role of TP53:72 polymorphism in Brazilian elderly people.

Distinct pattern of TP53 mutations in human immunodeficiency virus-related head and neck squamous cell carcinoma.

Science.gov (United States)

Gleber-Netto, Frederico O; Zhao, Mei; Trivedi, Sanchit; Wang, Jiping; Jasser, Samar; McDowell, Christina; Kadara, Humam; Zhang, Jiexin; Wang, Jing; William, William N; Lee, J Jack; Nguyen, Minh Ly; Pai, Sara I; Walline, Heather M; Shin, Dong M; Ferris, Robert L; Carey, Thomas E; Myers, Jeffrey N; Pickering, Curtis R

2018-01-01

Human immunodeficiency virus-infected individuals (HIVIIs) have a higher incidence of head and neck squamous cell carcinoma (HNSCC), and clinical and histopathological differences have been observed in their tumors in comparison with those of HNSCC patients without a human immunodeficiency virus (HIV) infection. The reasons for these differences are not clear, and molecular differences between HIV-related HNSCC and non-HIV-related HNSCC may exist. This study compared the mutational patterns of HIV-related HNSCC and non-HIV-related HNSCC. The DNA of 20 samples of HIV-related HNSCCs and 32 samples of non-HIV-related HNSCCs was sequenced. DNA libraries covering exons of 18 genes frequently mutated in HNSCC (AJUBA, CASP8, CCND1, CDKN2A, EGFR, FAT1, FBXW7, HLA-A, HRAS, KEAP1, NFE2L2, NOTCH1, NOTCH2, NSD1, PIK3CA, TGFBR2, TP53, and TP63) were prepared and sequenced on an Ion Personal Genome Machine sequencer. DNA sequencing data were analyzed with Ion Reporter software. The human papillomavirus (HPV) status of the tumor samples was assessed with in situ hybridization, the MassARRAY HPV multiplex polymerase chain reaction assay, and p16 immunostaining. Mutation calls were compared among the studied groups. HIV-related HNSCC revealed a distinct pattern of mutations in comparison with non-HIV-related HNSCC. TP53 mutation frequencies were significantly lower in HIV-related HNSCC. Mutations in HIV+ patients tended to be TpC>T nucleotide changes for all mutated genes but especially for TP53. HNSCC in HIVIIs presents a distinct pattern of genetic mutations, particularly in the TP53 gene. HIV-related HNSCC may have a distinct biology, and an effect of the HIV virus on the pathogenesis of these tumors should not be ruled out. Cancer 2018;124:84-94. © 2017 American Cancer Society. © 2017 American Cancer Society.

Significance of TP53 mutation in Wilms tumors with diffuse anaplasia : A report from the Children's Oncology Group

NARCIS (Netherlands)

Ooms, Ariadne H A G; Gadd, Samantha; Gerhard, Daniela S.; Smith, Malcolm A.; Guidry Auvil, Jaime M.; Meerzaman, Daoud; Chen, Qing Rong; Hsu, Chih Hao; Yan, Chunhua; Nguyen, Cu; Hu, Ying; Ma, Yussanne; Zong, Zusheng; Mungall, Andrew J.; Moore, Richard A.; Marra, Marco A.; Huff, Vicki; Dome, Jeffrey S.; Chi, Yueh Yun; Tian, Jing; Geller, James I.; Mullighan, Charles G.; Ma, Jing; Wheeler, David A.; Hampton, Oliver A.; Walz, Amy L.; Van Den Heuvel-Eibrink, Marry M.; De Krijger, Ronald R.; Ross, Nicole; Gastier-Foster, Julie M.; Perlman, Elizabeth J.

2016-01-01

Purpose: To investigate the role and significance of TP53 mutation in diffusely anaplastic Wilms tumors (DAWTs). Experimental Design: All DAWTs registered on National Wilms Tumor Study-5 (n = 118) with available samples were analyzed for TP53 mutations and copy loss. Integrative genomic analysis was

TP53 alterations in Wilms tumour represent progression events with strong intratumour heterogeneity that are closely linked but not limited to anaplasia.

Science.gov (United States)

Wegert, Jenny; Vokuhl, Christian; Ziegler, Barbara; Ernestus, Karen; Leuschner, Ivo; Furtwängler, Rhoikos; Graf, Norbert; Gessler, Manfred

2017-10-01

TP53 mutations have been associated with anaplasia in Wilms tumour, which conveys a high risk for relapse and fatal outcome. Nevertheless, TP53 alterations have been reported in no more than 60% of anaplastic tumours, and recent data have suggested their presence in tumours that do not fulfil the criteria for anaplasia, questioning the clinical utility of TP53 analysis. Therefore, we characterized the TP53 status in 84 fatal cases of Wilms tumour, irrespective of histological subtype. We identified TP53 alterations in at least 90% of fatal cases of anaplastic Wilms tumour, and even more when diffuse anaplasia was present, indicating a very strong if not absolute coupling between anaplasia and deregulation of p53 function. Unfortunately, TP53 mutations do not provide additional predictive value in anaplastic tumours since the same mutation rate was found in a cohort of non-fatal anaplastic tumours. When classified according to tumour stage, patients with stage I diffuse anaplastic tumours still had a high chance of survival (87%), but this rate dropped to 26% for stages II-IV. Thus, volume of anaplasia or possible spread may turn out to be critical parameters. Importantly, among non-anaplastic fatal tumours, 26% had TP53 alterations, indicating that TP53 screening may identify additional cases at risk. Several of these non-anaplastic tumours fulfilled some criteria for anaplasia, for example nuclear unrest, suggesting that such partial phenotypes should be under special scrutiny to enhance detection of high-risk tumours via TP53 screening. A major drawback is that these alterations are secondary changes that occur only later in tumour development, leading to striking intratumour heterogeneity that requires multiple biopsies and analysis guided by histological criteria. In conclusion, we found a very close correlation between histological signs of anaplasia and TP53 alterations. The latter may precede development of anaplasia and thereby provide diagnostic value

Overexpression of RBBP6, alone or combined with mutant TP53, is predictive of poor prognosis in colon cancer.

Directory of Open Access Journals (Sweden)

Jian Chen

Full Text Available Retinoblastoma binding protein 6 (RBBP6 plays an important role in chaperone-mediated ubiquitination and interacts with TP53 in carcinogenesis. However, the clinicopathologic significance of RBBP6 expression in colon cancer is unknown; in particular, the prognostic value of RBBP6 combined with TP53 expression has not been explored. Therefore, quantitative real-time PCR and western blot analyses were performed to detect RBBP6 expression in colon cancer tissues. RBBP6 and TP53 expression were assessed by immunohistochemistry in a tissue microarray format, in which the primary colon cancer tissue was paired with noncancerous tissue. Tissue specimens were obtained from 203 patients. We found that RBBP6 was overexpressed in colon tumorous tissues and was significantly associated with clinical stage, depth of tumor invasion, lymph node metastasis (LNM, distant metastasis, and histologic grade. Further studies revealed that a corresponding correlation between RBBP6 overexpression and mutant TP53 was evident in colon cancer (r = 0.450; P<0.001. RBBP6 expression was an independent prognostic factor for overall survival (OS and disease free survival (DFS. Interestingly, patients with tumors that had both RBBP6 overexpression and mutant TP53 protein accumulation relapsed and died within a significantly short period after surgery (P<0.001. Multivariate analysis showed that patients with LNM and patients with both RBBP6- and TP53-positive tumors had extremely poor OS (HR 6.75; 95% CI 2.63-17.35; P<0.001 and DFS (HR 8.08; 95% CI 2.80-23.30; P<0.001. These clinical findings indicate that the assessment of both RBBP6 and mutant TP53 expression will be helpful in predicting colon cancer prognosis.

Inactivation of TP53 correlates with disease progression and low miR-34a expression in previously treated chronic lymphocytic leukemia patients

DEFF Research Database (Denmark)

Dufour, Annika; Palermo, Giuseppe; Zellmeier, Evelyn

2013-01-01

In chronic lymphocytic leukemia (CLL) patients, disruptions of the TP53 tumor suppressor pathway by 17p13 deletion (del17p), somatic TP53 mutations, or downregulation of microRNA-34a have been associated with a poor prognosis. So far, the impact of the various TP53 defects has not been evaluated ...

Image File - TP Atlas | LSDB Archive [Life Science Database Archive metadata

Lifescience Database Archive (English)

Full Text Available ption of data contents Network diagrams (in PNG format) for each project. One project has one pathway file o...List Contact us TP Atlas Image File Data detail Data name Image File DOI 10.18908/lsdba.nbdc01161-004 Descri

Geochemical Characteristics of TP3 Mine Wastes at the Elizabeth Copper Mine Superfund Site, Orange County, Vermont

Science.gov (United States)

Hammarstrom, Jane M.; Piatak, Nadine M.; Seal, Robert R.; Briggs, Paul H.; Meier, Allen L.; Muzik, Timothy L.

2003-01-01

Remediation of the Elizabeth mine Superfund site in the Vermont copper belt poses challenges for balancing environmental restoration goals with issues of historic preservation while adopting cost-effective strategies for site cleanup and long-term maintenance. The waste-rock pile known as TP3, at the headwaters of Copperas Brook, is especially noteworthy in this regard because it is the worst source of surface- and ground-water contamination identified to date, while also being the area of greatest historical significance. The U.S. Geological Survey (USGS) conducted a study of the historic mine-waste piles known as TP3 at the Elizabeth mine Superfund site near South Strafford, Orange County, VT. TP3 is a 12.3-acre (49,780 m2) subarea of the Elizabeth mine site. It is a focus area for historic preservation because it encompasses an early 19th century copperas works as well as waste from late 19th- and 20th century copper mining (Kierstead, 2001). Surface runoff and seeps from TP3 form the headwaters of Copperas Brook. The stream flows down a valley onto flotation tailings from 20th century copper mining operations and enters the West Branch of the Ompompanoosuc River approximately 1 kilometer downstream from the mine site. Shallow drinking water wells down gradient from TP3 exceed drinking water standards for copper and cadmium (Hathaway and others, 2001). The Elizabeth mine was listed as a Superfund site in 2001, mainly because of impacts of acid-mine drainage on the Ompompanoosuc River.

[Cytogenetical comparison of restorers TP-4 and D minghui63 and maintainer D46B of autotetraploid rice].

Science.gov (United States)

Long, Wen-Bo; Luan, Li; Wang, Xing; Liu, Yu-Hua; Tu, Sheng-Bin; Kong, Fan-Lun; He, Tao

2007-04-01

Cytogenetical comparison was made between high seed set restorers TP-4 and D minghui63 and eminent maintainer line D46B of autotetraploid rice. The meiosis observation demonstrated the genomes of our autotetraploid materials were all 2n = 48, the same as those in mitosis observation. Low percentages of univalent and trivalent in metaphase I (MI) of restorers TP-4 and D minghui63 and in metaphase I (MI) of maintainer line D46B of autotetraploid rice were observed. And the percentages of chromosome pairing were all over 99%, showing eminent cytological character. The frequency of TP-4 and D minghui63 in metaphase I (MI) was 2.00/PMC and 2.26/PMC, respectively. However the frequency of D46B was 6.00/PMC, significantly higher than those of TP-4 and D minghui63. It indicated that the maintainer D46B has better chromosome pairing capability in metaphase I (MI). While, the frequency of lagging chromosomes of the maintainer D46B in anaphase I (AI) was 10.62%, significantly lower than that of TP-4 (19.44%) or D minghui63 (23.14%), and it was close to the level of diploid control (7.30%). In telophase I (TI), maintainer D46B exhibited a lower frequency of microkernel, and in telophase II (TII) the frequency of normal quartered microspore of maintainer D46B was not only higher than that of TP-4 or D minghui63 but also than that of diploid control. The percentage of the cell observed chromosome lagging in A1 and the percentage of abnormal cell in TI showed a greatly significant positive correlation. That may demonstrate chromo some separation in anaphase I (AI) and microkernel formation in telophase I (TI) are controlled by the same dominant single gene or the major gene of QTL.

Deletion/duplication mutation screening of TP53 gene in patients with transitional cell carcinoma of urinary bladder using multiplex ligation-dependent probe amplification.

Science.gov (United States)

Bazrafshani, Mohammad Reza R; Nowshadi, Pouriaali A; Shirian, Sadegh; Daneshbod, Yahya; Nabipour, Fatemeh; Mokhtari, Maral; Hosseini, Fatemehsadat; Dehghan, Somayeh; Saeedzadeh, Abolfazl; Mosayebi, Ziba

2016-02-01

Bladder cancer is a molecular disease driven by the accumulation of genetic, epigenetic, and environmental factors. The aim of this study was to detect the deletions/duplication mutations in TP53 gene exons using multiplex ligation-dependent probe amplification (MLPA) method in the patients with transitional cell carcinoma (TCC). The achieved formalin-fixed paraffin-embedded tissues from 60 patients with TCC of bladder were screened for exonal deletions or duplications of every 12 TP53 gene exons using MLPA. The pathological sections were examined by three pathologists and categorized according to the WHO scoring guideline as 18 (30%) grade I, 22 (37%) grade II, 13 (22%) grade III, and 7 (11%) grade IV cases of TCC. None mutation changes of TP53 gene were detected in 24 (40%) of the patients. Furthermore, mutation changes including, 15 (25%) deletion, 17 (28%) duplication, and 4 (7%) both deletion and duplication cases were observed among 60 samples. From 12 exons of TP53 gene, exon 1 was more subjected to exonal deletion. Deletion of exon 1 of TP53 gene has occurred in 11 (35.4%) patients with TCC. In general, most mutations of TP53, either deletion or duplication, were found in exon 1, which was statistically significant. In addition, no relation between the TCC tumor grade and any type of mutation were observed in this research. MLPA is a simple and efficient method to analyze genomic deletions and duplications of all 12 exons of TP53 gene. The finding of this report that most of the mutations of TP53 occur in exon 1 is in contrast to that of the other reports suggesting that exons 5-8 are the most (frequently) mutated exons of TP53 gene. The mutations of exon 1 of TP53 gene may play an important role in the tumorogenesis of TCC. © 2015 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.

T/P23, 24, 911 and 92: New grades for advanced coal-fired power plants-Properties and experience

International Nuclear Information System (INIS)

Vaillant, J.C.; Vandenberghe, B.; Hahn, B.; Heuser, H.; Jochum, C.

2008-01-01

After the development of the well-known T/P91 grade in the early 1980s and the long industrial experiences since the early 1990s, it has been necessary to develop new steels to answer the demand of the powergen industry. New (ultra) super critical boilers require materials with advanced creep properties to reach severe steam parameters. For the 2.25% Cr family, grades 23 and 24 are now available to be used instead of the previous grade 22 with many technical and economical advantages. For the 9% Cr steels, new grades such as T/P911 and T/P92 have been developed with higher creep properties than T/P91. Thanks to its large industrial experience in these new grades, Vallourec and Mannesmann Tubes and Boehler Thyssen Schweisstechnik have now worldwide references of use with these new steel grades. In this paper, information on properties, behaviour and workability will be presented with a focus on ·elevated temperature properties and allowable stresses, ·oxidation and maximum service temperature and ·weldability procedures and recommendations

Involvement of phorbol-12-myristate-13-acetate-induced protein 1 in goniothalamin-induced TP53-dependent and -independent apoptosis in hepatocellular carcinoma-derived cells

International Nuclear Information System (INIS)

Kuo, Kung-Kai; Chen, Yi-Ling; Chen, Lih-Ren; Li, Chien-Feng; Lan, Yu-Hsuan; Chang, Fang-Rong; Wu, Yang-Chang; Shiue, Yow-Ling

2011-01-01

The objective was to investigate the upstream apoptotic mechanisms that were triggered by a styrylpyrone derivative, goniothalamin (GTN), in tumor protein p53 (TP53)-positive and -negative hepatocellular carcinoma (HCC)-derived cells. Effects of GTN were evaluated by the flow cytometry, alkaline comet assay, immunocytochemistry, small-hairpin RNA interference, mitochondria/cytosol fractionation, quantitative reverse transcription-polymerase chain reaction, immunoblotting analysis and caspase 3 activity assays in two HCC-derived cell lines. Results indicated that GTN triggered phorbol-12-myristate-13-acetate-induced protein 1 (PMAIP1, also known as NOXA)-mediated apoptosis via TP53-dependent and -independent pathways. In TP53-positive SK-Hep1 cells, GTN furthermore induced TP53 transcription-dependent and -independent apoptosis. After GTN treatment, accumulation of reactive oxygen species, formation of DNA double-strand breaks, transactivation of TP53 and/or PMAIP1 gene, translocation of TP53 and/or PMAIP1 proteins to mitochondria, release of cytochrome c from mitochondria, cleavage of caspases and induction of apoptosis in both cell lines were sustained. GTN might represent a novel class of anticancer drug that induces apoptosis in HCC-derived cells through PMAIP1 transactivation regardless of the status of TP53 gene. - Highlights: → Goniothalamin (GTN) induced apoptosis in hepatocellular carcinomas-derived cells. → The apoptosis induced by GTN is PMAIP1-dependent, regardless of TP53 status. → The apoptosis induced by GTN might be TP53 transcription-dependent or -independent. → GTN-induced apoptosis is mitochondria- and caspases-mediated.

Influencia de la responsabilidad social en la lealtad: aplicación en el mercado de servicios

OpenAIRE

García de los Salmones Sánchez, María del Mar; Rodríguez del Bosque Rodríguez, Ignacio Alfredo

2011-01-01

El presente trabajo analiza la influencia de la responsabilidad social corporativa en el comportamiento del consumidor mediante su inclusión en los modelos tradicionales de lealtad. Para ello, se parte de una revisión de la literatura sobre el concepto que da pie al planteamiento de varias hipótesis de investigación relativas a la influencia del comportamiento socialmente responsable en la valoración de un servicio y en la imagen corporativa, antecedentes directos, junto con...

Valproic acid treatment response in vitro is determined by TP53 status in medulloblastoma.

Science.gov (United States)

Mascaro-Cordeiro, Bruna; Oliveira, Indhira Dias; Tesser-Gamba, Francine; Pavon, Lorena Favaro; Saba-Silva, Nasjla; Cavalheiro, Sergio; Dastoli, Patrícia; Toledo, Silvia Regina Caminada

2018-05-22

Histone deacetylate inhibitors (HDACi), as valproic acid (VA), have been reported to enhance efficacy and to prevent drug resistance in some tumors, including medulloblastoma (MB). In the present study, we investigated VA role, combined to cisplatin (CDDP) in cell viability and gene expression of MB cell lines. Dose-response curve determined IC 50 values for each treatment: (1) VA single, (2) CDDP single, and (3) VA and CDDP combined. Cytotoxicity and flow cytometry evaluated cell viability after exposure to treatments. Quantitative PCR evaluated gene expression levels of AKT, CTNNB1, GLI1, KDM6A, KDM6B, NOTCH2, PTCH1, and TERT, before and after treatment. Besides, we performed next-generation sequencing (NGS) for PTCH1, TERT, and TP53 genes. The most effective treatment to reduce viability was combined for D283MED and ONS-76; and CDDP single for DAOY cells (p AKT genes were overexpressed after treatments with VA. D283MED and ONS-76 cells presented variants in TERT and PTCH1, respectively and DAOY cell line presented a TP53 mutation. MB tumors belonging to SHH molecular subgroup, with TP53 MUT , would be the ones that present high risk in relation to VA use during the treatment, while TP53 WT MBs can benefit from VA therapy, both SHH and groups 3 and 4. Our study shows a new perspective about VA action in medulloblastoma cells, raising the possibility that VA may act in different patterns. According to the genetic background of MB cell, VA can stimulate cell cycle arrest and apoptosis or induce resistance to treatment via signaling pathways activation.

Predictive and prognostic impact of TP53 mutations and MDM2 promoter genotype in primary breast cancer patients treated with epirubicin or paclitaxel.

Directory of Open Access Journals (Sweden)

Ranjan Chrisanthar

Full Text Available BACKGROUND: TP53 mutations have been associated with resistance to anthracyclines but not to taxanes in breast cancer patients. The MDM2 promoter single nucleotide polymorphism (SNP T309G increases MDM2 activity and may reduce wild-type p53 protein activity. Here, we explored the predictive and prognostic value of TP53 and CHEK2 mutation status together with MDM2 SNP309 genotype in stage III breast cancer patients receiving paclitaxel or epirubicin monotherapy. EXPERIMENTAL DESIGN: Each patient was randomly assigned to treatment with epirubicin 90 mg/m(2 (n = 109 or paclitaxel 200 mg/m(2 (n = 114 every 3rd week as monotherapy for 4-6 cycles. Patients obtaining a suboptimal response on first-line treatment requiring further chemotherapy received the opposite regimen. Time from last patient inclusion to follow-up censoring was 69 months. Each patient had snap-frozen tumor tissue specimens collected prior to commencing chemotherapy. PRINCIPAL FINDINGS: While TP53 and CHEK2 mutations predicted resistance to epirubicin, MDM2 status did not. Neither TP53/CHEK2 mutations nor MDM2 status was associated with paclitaxel response. Remarkably, TP53 mutations (p = 0.007 but also MDM2 309TG/GG genotype status (p = 0.012 were associated with a poor disease-specific survival among patients having paclitaxel but not patients having epirubicin first-line. The effect of MDM2 status was observed among individuals harbouring wild-type TP53 (p = 0.039 but not among individuals with TP53 mutated tumors (p>0.5. CONCLUSION: TP53 and CHEK2 mutations were associated with lack of response to epirubicin monotherapy. In contrast, TP53 mutations and MDM2 309G allele status conferred poor disease-specific survival among patients treated with primary paclitaxel but not epirubicin monotherapy.

TP53 hotspot mutations are predictive of survival in primary central nervous system lymphoma patients treated with combination chemotherapy

DEFF Research Database (Denmark)

Munch-Petersen, Helga D; Asmar, Fazila; Dimopoulos, Konstantinos

2016-01-01

regimens (high-dose methotrexate/whole brain radiation therapy, 6.0 months, or no therapy, 0.83 months), P hotspot/direct DNA contact mutations. CCT-treated patients with PCNSL harboring a hotspot/direct DNA contact MUT......-TP53 (n = 9) had a significantly worse OS and progression free survival (PFS) compared to patients with non-hotspot/non-direct DNA contact MUT-TP53 or wild-type TP53 (median PFS 4.6 versus 18.2 or 45.7 months), P = 0.041 and P = 0.00076, respectively. Multivariate Cox regression analysis confirmed...... that hotspot/direct DNA contact MUT-TP53 was predictive of poor outcome in CCT-treated PCNSL patients, P = 0.012 and P = 0.008; HR: 1.86 and 1.95, for OS and PFS, respectively. MIR34A, MIR34B/C, and DAPK promoter methylation were detected in 53/93 (57.0 %), 80/84 (95.2 %), and 70/75 (93.3 %) of the PCNSL...

Sequential mutations in Notch1, Fbxw7, and Tp53 in radiation-induced mouse thymic lymphomas.

Science.gov (United States)

Jen, Kuang-Yu; Song, Ihn Young; Banta, Karl Luke; Wu, Di; Mao, Jian-Hua; Balmain, Allan

2012-01-19

T-cell acute lymphoblastic lymphomas commonly demonstrate activating Notch1 mutations as well as mutations or deletions in Fbxw7. However, because Fbxw7 targets Notch1 for degradation, genetic alterations in these genes are expected to be mutually exclusive events in lymphomagenesis. Previously, by using a radiation-induced Tp53-deficient mouse model for T-cell acute lymphoblastic lymphoma, we reported that loss of heterozygosity at the Fbxw7 locus occurs frequently in a Tp53-dependent manner. In the current study, we show that these thymic lymphomas also commonly exhibit activating Notch1 mutations in the proline-glutamic acid-serine-threonine (PEST) domain. Moreover, concurrent activating Notch1 PEST domain mutations and single-copy deletions at the Fbxw7 locus occur with high frequency in the same individual tumors, indicating that these changes are not mutually exclusive events. We further demonstrate that although Notch1 PEST domain mutations are independent of Tp53 status, they are completely abolished in mice with germline Fbxw7 haploinsufficiency. Therefore, Notch1 PEST domain mutations only occur when Fbxw7 expression levels are intact. These data suggest a temporal sequence of mutational events involving these important cancer-related genes, with Notch1 PEST domain mutations occurring first, followed by Fbxw7 deletion, and eventually by complete loss of Tp53.

Polimorfismo do gene tp53 no códon 72 em pacientes com suspeita de LMC Codon 72 polymorphism of the TP53 gene in patients suspected to have CML

Directory of Open Access Journals (Sweden)

Camila S. Hamú

2007-12-01

Full Text Available A leucemia mielóide crônica (LMC é uma doença proliferativa do sistema hematopoiético, caracterizada pela expansão clonal de uma célula-tronco primitiva e pluripotente denominada stem cell. Este tipo de leucemia está associado, em 90% dos casos, à translocação t(9;22(q34;q11. Essa alteração cromossômica estrutural codifica para uma proteína quimérica BCR-ABL, que confere às células leucêmicas uma alta resistência à morte, independente do agente indutor desse processo. A proteína p53 é uma reguladora transcricional induzida por danos no DNA, fato que resulta na parada do ciclo celular com conseqüente ativação de mecanismos de reparo ou mesmo na indução à apoptose. As mutações no gene TP53 são as alterações genéticas mais comuns em tumores malignos humanos. O presente estudo teve como objetivo genotipar e determinar a freqüência alélica do polimorfismo do TP53 no códon 72 (arginina - Arg e prolina - Pro, em pacientes com suspeita de LMC, pela Reação em Cadeia da Polimerase. Desta forma, os resultados indicaram que 73,4% (23/30 dos pacientes apresentaram homozigose para arginina (Arg/Arg e 26,6% (7/30 heterozigose (Arg/Pro. Não foi encontrado nenhum paciente homozigoto para prolina (Pro/Pro. Os resultados obtidos sugerem que o polimorfismo do gene TP53 no códon 72 não é um fator de risco importante para a iniciação, promoção e progressão da LMC.Chronic myeloid leukemia (CML is a proliferative disorder of the hematopoietic system characterized by clonal expansion of a primitive and pluripotent stem cell. In this type of leukemia, up to 90% of all cases is associated to a specific chromosomal translocation, t(9;22(q34;q11. The genomic alteration results in a chimeric protein, BCR-ABL, that confers a high resistance leukemia cells to death, independent of the induction mechanism of this process. Protein p53 is a transcriptional factor expressed after DNA damage which ceases cell cycle progression and

Key Players in the Genetic Switch of Bacteriophage TP901-1

DEFF Research Database (Denmark)

Alsing, Anne; Pedersen, Margit; Sneppen, Kim

2011-01-01

the bistable genetic switch of bacteriophage TP901-1 through experiments and statistical mechanical modeling. We examine the activity of the lysogenic promoter Pr at different concentrations of the phage repressor, CI, and compare the effect of CI on Pr in the presence or absence of the phage-encoded MOR...

Influencia del Pseudo Dionisio en Nicolás de Cusa

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Andrés Hubert R

2007-01-01

Full Text Available Nicolás de Cusa (1401-1464 reconoce la influencia del Pseudo Dionisio en su propio pensamiento, especialmente en su teología negativa. Este trabajo busca mostrar esta influencia en el libro referencia del Cusano: el De Docta Ignorantia (la Docta Ignorancia. Esta obra consta de 3 partes. Solo en la primera parte, Nicolás sigue de cerca las ideas del Pseudo Dionisio y solo en ella lo cita. En las demás, aunque se nota la influencia, nuestro autor se siente más libre: las fundaciones están firmes, el constructor puede construir libremente. Para probar esta tesis, se toman dos ejemplos: el tema de las tinieblas y la coincidencia de los opuestos. Este ejercicio permite conocer cómo y por qué se recibió al Pseudo Dionisio en la historia de la teología. Permite también profundizar el sentido de la teología de Nicolás de CusaThe reflections of Nicolas of Cusa (1401-1464 recognise the influence of Pseudo Dyonisius, particularly in his negative theology. The present study shows this influence on Nicolas' most important book: the De Docta Ignorantia (the Learned Ignorance. The book is divided into three parts and it is in the first part that Nicolas directly pursues the ideas of Pseudo Dyonisius and it is only here that he quotes them. In the other parts of the book although the influence is noted, the foundations have been set and are solid and the author or builder can construct with freedom. To prove this thesis, 2 examples are presented: the theme of darkness and the coincidence of opposites. This exercise shows how and why Pseudo Dyonisius has been accepted into the history of the theology. It also deepens the meaning of the Nicolas of Cusa's theology

TFP5/TP5 peptide provides neuroprotection in the MPTP model of Parkinson′s disease

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B K Binukumar

2016-01-01

Full Text Available Cyclin-dependent kinase 5 (Cdk5 is a member of the serine-threonine kinase family of cyclin-dependent kinases. Cdk5 is critical to normal mammalian nervous system development and plays important regulatory roles in multiple cellular functions. Recent evidence indicates that Cdk5 is inappropriately activated in several neurodegenerative conditions, including Parkinson′s disease (PD. PD is a chronic neurodegenerative disorder characterized by the loss of dopamine neurons in the substantia nigra, decreased striatal dopamine levels, and consequent extrapyramidal motor dysfunction. During neurotoxicity, p35 is cleaved to form p25. Binding of p25 with Cdk5 leads deregulation of Cdk5 resulting in number of neurodegenerative pathologies. To date, strategies to specifically inhibit Cdk5 hyperactivity have not been successful without affecting normal Cdk5 activity. Here we show that inhibition of p25/Cdk5 hyperactivation through TFP5/TP5, truncated 24-aa peptide derived from the Cdk5 activator p35 rescues nigrostriatal dopaminergic neurodegeneration induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP/MPP + in a mouse model of PD. TP5 peptide treatment also blocked dopamine depletion in the striatum and improved gait dysfunction after MPTP administration. The neuroprotective effect of TFP5/TP5 peptide is also associated with marked reduction in neuroinflammation and apoptosis. Here we show inhibition of Cdk5/p25-hyperactivation by TFP5/TP5 peptide, which identifies Cdk5/p25 as a potential therapeutic target to reduce neurodegeneration in PD.

Los niños, la influencia de la televisión y el rendimiento escolar

OpenAIRE

Pauta Guevara, Ricardo Arturo

2015-01-01

La presente investigación titulada “Los niños, la influencia de la televisión y el rendimiento escolar”, tiene como objetivo general mostrar la influencia que ejerce la Televisión en el comportamiento y en el rendimiento escolar de los niños de 6 a 9 años de edad, de clase media de Lima. Los medios de comunicación hoy en día, son parte de nuestra vida. Nos acompañan y entretienen, ponen a nuestro alcance lo ajeno y lejano, alientan nuestra imaginación con personajes e historias, nos mantienen...

Integration of TP53, DREAM, MMB-FOXM1 and RB-E2F target gene analyses identifies cell cycle gene regulatory networks.

Science.gov (United States)

Fischer, Martin; Grossmann, Patrick; Padi, Megha; DeCaprio, James A

2016-07-27

Cell cycle (CC) and TP53 regulatory networks are frequently deregulated in cancer. While numerous genome-wide studies of TP53 and CC-regulated genes have been performed, significant variation between studies has made it difficult to assess regulation of any given gene of interest. To overcome the limitation of individual studies, we developed a meta-analysis approach to identify high confidence target genes that reflect their frequency of identification in independent datasets. Gene regulatory networks were generated by comparing differential expression of TP53 and CC-regulated genes with chromatin immunoprecipitation studies for TP53, RB1, E2F, DREAM, B-MYB, FOXM1 and MuvB. RNA-seq data from p21-null cells revealed that gene downregulation by TP53 generally requires p21 (CDKN1A). Genes downregulated by TP53 were also identified as CC genes bound by the DREAM complex. The transcription factors RB, E2F1 and E2F7 bind to a subset of DREAM target genes that function in G1/S of the CC while B-MYB, FOXM1 and MuvB control G2/M gene expression. Our approach yields high confidence ranked target gene maps for TP53, DREAM, MMB-FOXM1 and RB-E2F and enables prediction and distinction of CC regulation. A web-based atlas at www.targetgenereg.org enables assessing the regulation of any human gene of interest. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

Identification of operator sites of the CI repressor of phage TP901-1: evolutionary link to other phages

International Nuclear Information System (INIS)

Johansen, Annette H.; Broendsted, Lone; Hammer, Karin

2003-01-01

The repressor encoded by the cI gene of the temperate Lactococcus lactis subsp. cremoris bacteriophage TP901-1 has been purified. Gel-retardation and footprinting analyses identified three palindromic operator sites (O R , O L , and O D ). The operator site O R is located between the two divergent early promoters P R and P L , O L overlaps the transcriptional start of the lytic P L promoter, and O D is located downstream of the mor gene, the first gene in the lytic gene cluster. The function of O L was verified by mutational analysis. Binding was found to be specific and cooperative. Multimeric forms of the repressor were observed, thus indicating that the repressor may bind simultaneously to all three operator sites. Inverted repeats with homology to the operator sites of TP901-1 were identified in phage genomes encoding repressors homologous to CI of TP901-1. Interestingly, the locations of these repeats on the phage genomes correspond to those found in TP901-1, indicating that the same system of cooperative repression of early phage promoters has been inherited by modular evolution

La influencia externa en las políticas educativas de Argentina, Brasil y Chile (1990-2006)

OpenAIRE

Rambla, Francesc Xavier,

2009-01-01

El objetivo del artículo es analizar el papel que juega la influencia internacional en el diseño y aplicación de políticas educativas en el Cono Sur. Específicamente pretende explorar el proceso a través del cual las ideas, recomendaciones y metas globales modelan las políticas educativas latinoamericanas. Para ello, el artículo se centra en el programa de Educación para Todos en el Acuerdo General de Comercio de Servicios, analizando los mecanismos de influencia externa que intervienen en ca...

Baseline results from the UK SIGNIFY study: a whole-body MRI screening study in TP53 mutation carriers and matched controls.

Science.gov (United States)

Saya, Sibel; Killick, Emma; Thomas, Sarah; Taylor, Natalie; Bancroft, Elizabeth K; Rothwell, Jeanette; Benafif, Sarah; Dias, Alexander; Mikropoulos, Christos; Pope, Jenny; Chamberlain, Anthony; Gunapala, Ranga; Izatt, Louise; Side, Lucy; Walker, Lisa; Tomkins, Susan; Cook, Jackie; Barwell, Julian; Wiles, Vicki; Limb, Lauren; Eccles, Diana; Leach, Martin O; Shanley, Susan; Gilbert, Fiona J; Hanson, Helen; Gallagher, David; Rajashanker, Bala; Whitehouse, Richard W; Koh, Dow-Mu; Sohaib, S Aslam; Evans, D Gareth; Eeles, Rosalind A

2017-07-01

In the United Kingdom, current screening guidelines for TP53 germline mutation carriers solely recommends annual breast MRI, despite the wide spectrum of malignancies typically seen in this group. This study sought to investigate the role of one-off non-contrast whole-body MRI (WB MRI) in the screening of asymptomatic TP53 mutation carriers. 44 TP53 mutation carriers and 44 population controls were recruited. Scans were read by radiologists blinded to participant carrier status. The incidence of malignancies diagnosed in TP53 mutation carriers against general population controls was calculated. The incidences of non-malignant relevant disease and irrelevant disease were measured, as well as the number of investigations required to determine relevance of findings. In TP53 mutation carriers, 6 of 44 (13.6, 95% CI 5.2-27.4%) participants were diagnosed with cancer during the study, all of which would be considered life threatening if untreated. Two were found to have two primary cancers. Two participants with cancer had abnormalities on the MRI which were initially thought to be benign (a pericardial cyst and a uterine fibroid) but transpired to be sarcomas. No controls were diagnosed with cancer. Fifteen carriers (34.1, 95% CI 20.5-49.9%) and seven controls (15.9, 95% CI 6.7-30.1%) underwent further investigations following the WB MRI for abnormalities that transpired to be benign (p = 0.049). The cancer detection rate in this group justifies a minimum baseline non-contrast WB MRI in germline TP53 mutation carriers. This should be adopted into national guidelines for management of adult TP53 mutation carriers in addition to the current practice of contrast enhanced breast MRI imaging.

Reactivating TP53 signaling by the novel MDM2 inhibitor DS-3032b as a therapeutic option for high-risk neuroblastoma

Science.gov (United States)

Arnhold, Viktor; Schmelz, Karin; Proba, Jutta; Winkler, Annika; Wünschel, Jasmin; Toedling, Joern; Deubzer, Hedwig E.; Künkele, Annette; Eggert, Angelika; Schulte, Johannes H.; Hundsdoerfer, Patrick

2018-01-01

Fewer than 50% of patients with high-risk neuroblastoma survive five years after diagnosis with current treatment protocols. Molecular targeted therapies are expected to improve survival. Although MDM2 has been validated as a promising target in preclinical models, no MDM2 inhibitors have yet entered clinical trials for neuroblastoma patients. Toxic side effects, poor bioavailability and low efficacy of the available MDM2 inhibitors that have entered phase I/II trials drive the development of novel MDM2 inhibitors with an improved risk-benefit profile. We investigated the effect of the novel MDM2 small molecular inhibitor, DS-3032b, on viability, proliferation, senescence, migration, cell cycle arrest and apoptosis in a panel of six neuroblastoma cell lines with different TP53 and MYCN genetic backgrounds, and assessed efficacy in a murine subcutaneous model for high-risk neuroblastoma. Re-analysis of existing expression data from 476 primary neuroblastomas showed that high-level MDM2 expression correlated with poor patient survival. DS-3032b treatment enhanced TP53 target gene expression and induced G1 cell cycle arrest, senescence and apoptosis. CRISPR-mediated MDM2 knockout in neuroblastoma cells mimicked DS-3032b treatment. TP53 signaling was selectively activated by DS-3032b in neuroblastoma cells with wildtype TP53, regardless of the presence of MYCN amplification, but was significantly reduced by TP53 mutations or expression of a dominant-negative TP53 mutant. Oral DS-3032b administration inhibited xenograft tumor growth and prolonged mouse survival. Our in vitro and in vivo data demonstrate that DS-3032b reactivates TP53 signaling even in the presence of MYCN amplification in neuroblastoma cells, to reduce proliferative capacity and cause cytotoxicity. PMID:29416773

Influencia de Gregorio de Nisa sobre Juan Escoto Eriúgena: Aproximación a partir del Periphyseon

OpenAIRE

Pachas, José Antonio

2004-01-01

A partir de los cinco libros del Periphyseon el autor intenta un acercamiento entre el pensamiento de Juan Escoto Eriúgena y Gregorio de Nisa, buscando sobre todo las principales influencias del segundo sobre el primero. Encuentra una plataforma de amplia base común entre ambos pensadores, marcada principalmente por un tipo de pensamiento circular según el esquema: procesión y retorno. Sin embargo, es notoria la influencia del Niseno sobre el Carolingio, principalmente cuando se trata de plan...

Una retórica de la influencia

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Cecilia González

2008-07-01

Full Text Available Entre las últimas décadas del siglo XIX y las primeras del XX se publica en el Río de la Plata un número considerable de relatos anclados en los debates que recorren la cultura europea del momento y que giran en torno a fenómenos de influencia. Este denominador común se declina, según los textos, en una serie de temas privilegiados : hipnosis, sugestión, herencia, mimetismo, vampirismo, parasitismo, emanación de todo tipo de rayos, ondas y fluidos capaces de modificar el comportamiento de un ...

INFLUENCIA DE LA PRESIÓN ATMOSFÉRICA EN LAS TRANSMISIONES A FRECUENCIA 2.4 GHz

OpenAIRE

González Romero, Pedro Vicente; Espina Ávila, Luis Gerardo

2012-01-01

La presente investigación se planteó como propósito fundamental determinar la influencia de la presión atmosférica sobre una señal transmitida en una frecuencia de 2.4 GHz. La investigación es de tipo explicativa, con diseño metodológico experimental. Mediante ella se obtiene la atenuación de una señal de 2.4GHz a diferentes presiones atmosféricas y se determinan las gráficas que muestran el comportamiento observado. Como resultado de esta investigación se observó que sí existe una influencia...

La influencia social de los medios de comunicación en Twitter

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Karines Rodríguez Díaz

2017-06-01

Full Text Available En este artículo se expone cómo se desarrolla la influencia social de los medios de comunicación sobre los usuarios en Twitter, durante la celebración de la VII Cumbre de la Asociación de Estados del Caribe (AEC. La investigación parte del análisis de un corpus de 1500 tuits con las etiquetas temáticas #CubaCaribe, #VIICumbreAEC, #CumbreAEC. Utilizando el análisis del discurso mediado por computador, los resultados muestran la habilidad de influencia de los medios de comunicación a través de patrones de repetición-reacción y en la construcción del consenso sobre la valoración del acontecimiento. El reporte de caso explica cómo los parlamentos mediáticos repercuten en la comunidad de usuarios en el contexto microblogging.

Expression of TpNRAMP5, a metal transporter from Polish wheat (Triticum polonicum L.), enhances the accumulation of Cd, Co and Mn in transgenic Arabidopsis plants.

Science.gov (United States)

Peng, Fan; Wang, Chao; Zhu, Jianshu; Zeng, Jian; Kang, Houyang; Fan, Xing; Sha, Lina; Zhang, Haiqin; Zhou, Yonghong; Wang, Yi

2018-06-01

TpRNAMP5 is mainly expressed in the plasma membrane of roots and basal stems. It functions as a metal transporter for Cd, Mn and Co accumulation. Numerous natural resistance-associated macrophage proteins (NRAMPs) have been functionally identified in various plant species, including Arabidopsis, rice, soybean and tobacco, but no information is available on NRAMP genes in wheat. In this study, we isolated a TpNRAMP5 from dwarf Polish wheat (DPW, Triticum polonicum L.), a species with high tolerance to Cd and Zn. Expression pattern analysis revealed that TpNRAMP5 is mainly expressed in roots and basal stems of DPW. TpNRAMP5 was localized at the plasma membrane of Arabidopsis leaf protoplast. Expression of TpNRAMP5 in yeast significantly increased yeast sensitivity to Cd and Co, but not Zn, and enhanced Cd and Co concentrations. Expression of TpNRAMP5 in Arabidopsis significantly increased Cd, Co and Mn concentrations in roots, shoots and whole plants, but had no effect on Fe and Zn concentrations. These results indicate that TpNRAMP5 is a metal transporter enhancing the accumulation of Cd, Co and Mn, but not Zn and Fe. Genetic manipulation of TpNRAMP5 can be applied in the future to limit the transfer of Cd from soil to wheat grains, thereby protecting human health.

Novel variant in the TP63 gene associated to ankyloblepharon-ectodermal dysplasia-cleft lip/palate (AEC) syndrome.

Science.gov (United States)

Gonzalez, Francisco; Loidi, Lourdes; Abalo-Lojo, Jose M

2017-01-01

Ankyloblepharon-ectodermal dysplasia-cleft lip/palate (AEC) syndrome is a disorder resulting from anomalous embryonic development of ectodermal tissues. There is evidence that AEC syndrome is caused by mutations in the TP63 gene, which encodes the p63 protein. This is an important regulatory protein involved in epidermal proliferation and differentiation. Genome sequencing was performed in DNA from peripheral blood leukocytes of a newborn with AEC syndrome and her parents. Variants were searched in all coding exons and intron-exon boundaries of the TP63 gene. A heterozygous missense variant (NM_003722.4:c.1063G>C (p.Asp355His) was found in the newborn patient. No variants were found in either of the parents. We identified a previously unreported variant in TP63 gene which seems to be involved in the somatic malformations found in the AEC syndrome. The absence of this variant in both parents suggests that the variant appeared de novo.

BRASIL BAJO INFLUENCIA NAPOLEÓNICA Y FRANCESA: LOS MENSAJEROS DE LA INDEPENDENCIA: MILITARES, LIBREROS Y PERIODISTAS

OpenAIRE

PUIGMAL, PATRICK

2013-01-01

La historiografía clásica presenta la independencia de Brasil como un evento particular, generalmente aislado del acontecer continental y sin hacer referencia a la influencia militar y política napoleónica. Este artículo tiene como propósito principal entender la emancipación brasileña en un contexto mucho más amplio y demostrar la amplia presencia e influencia napoleónica en el marco de la lucha militar y de la construcción del Estado nuevo, permitiendo así integrar a Brasil en un movimiento...

CK2 phospho-dependent binding of R2TP complex to TEL2 is essential for mTOR and SMG1 stability.

Science.gov (United States)

Horejsí, Zuzana; Takai, Hiroyuki; Adelman, Carrie A; Collis, Spencer J; Flynn, Helen; Maslen, Sarah; Skehel, J Mark; de Lange, Titia; Boulton, Simon J

2010-09-24

TEL2 interacts with and is essential for the stability of all phosphatidylinositol 3-kinase-related kinases (PIKKs), but its mechanism of action remains unclear. Here, we show that TEL2 is constitutively phosphorylated on conserved serines 487 and 491 by casein kinase 2 (CK2). Proteomic analyses establish that the CK2 phosphosite of TEL2 confers binding to the R2TP/prefoldin-like complex, which possesses chaperon/prefoldin activities required during protein complex assembly. The PIH1D1 subunit of the R2TP complex binds directly to the CK2 phosphosite of TEL2 in vitro and is required for the TEL2-R2TP/prefoldin-like complex interaction in vivo. Although the CK2 phosphosite mutant of TEL2 retains association with the PIKKs and HSP90 in cells, failure to interact with the R2TP/prefoldin-like complex results in instability of the PIKKs, principally mTOR and SMG1. We propose that TEL2 acts as a scaffold to coordinate the activities of R2TP/prefoldin-like and HSP90 chaperone complexes during the assembly of the PIKKs. Copyright © 2010 Elsevier Inc. All rights reserved.

Radiosensitivity and TP 53, EGFR amplification and LOH10 analysis of primary glioma cell cultures

International Nuclear Information System (INIS)

Gerlach, B.; Harder, A.H.; Slotman, B.J.; Sminia, P.; Hulsebos, T.J.M.; Leenstra, S.; Peter Vandertop, W.; Hartmann, K.A.

2002-01-01

Aim: Determination of in-vitro radiosensitivity and genetic alterations of cell cultures derived from human glioma biopsy tissue and established glioma cell lines. Material and Methods: Fresh brain tumor specimens of six patients were processed to early passage cell cultures. In addition the cell lines D 384 and Gli 6 were used. Cell cultures were irradiated with doses from 2 to 10 Gy. Following irradiation, cell survival was determined by clonogenic assay and survival curves were generated. The surviving fractions after 2 Gy (SF2) and 4 Gy (SF4) were used as radiosensitivity parameters. Genetic analysis included determination of the mutational and loss of heterozygosity (LOH) status of TP 53 (exons 5-8), the LOH 10- and epidermal growth factor receptor gene (EGFR) amplification status. Results: The SF2 and SF4 values ranged from 0.54 to 0.88 (mean: 0.70) and from 0.13 to 0.52 (mean: 0.32), respectively. Genetic alterations were found in the Gli 6 cell line and in two primary cell cultures. The genetic profile of Gli 6 showed LOH but no TP 53 mutation, complete LOH 10 and no EGFR amplification. The VU 15 cell culture showed TP 53 mutation but no LOH 10 or EGFR amplification, while VU 24 showed incomplete LOH 10, EGFR amplification and no TP 53 mutation. In the other four cell cultures and D 384 cell line no genetic alterations were diagnosed. Histopathological classification of glioblastoma multiforme and/or genetic alterations resulted in lower radiosensitivity. Conclusion: In this small series of early passage glioma cell cultures low radiosensitivity and alterations in cell regulatory genes were seen. Further testing of biological behavior in larger series of patient-derived material is ongoing. (orig.)

Benzo[a]pyrene, Aflatoxine B1 and Acetaldehyde Mutational Patterns in TP53 Gene Using a Functional Assay: Relevance to Human Cancer Aetiology

Science.gov (United States)

Paget, Vincent; Lechevrel, Mathilde; André, Véronique; Le Goff, Jérémie; Pottier, Didier; Billet, Sylvain; Garçon, Guillaume; Shirali, Pirouz; Sichel, François

2012-01-01

Mutations in the TP53 gene are the most common alterations in human tumours. TP53 mutational patterns have sometimes been linked to carcinogen exposure. In hepatocellular carcinoma, a specific G>T transversion on codon 249 is classically described as a fingerprint of aflatoxin B1 exposure. Likewise G>T transversions in codons 157 and 158 have been related to tobacco exposure in human lung cancers. However, controversies remain about the interpretation of TP53 mutational pattern in tumours as the fingerprint of genotoxin exposure. By using a functional assay, the Functional Analysis of Separated Alleles in Yeast (FASAY), the present study depicts the mutational pattern of TP53 in normal human fibroblasts after in vitro exposure to well-known carcinogens: benzo[a]pyrene, aflatoxin B1 and acetaldehyde. These in vitro patterns of mutations were then compared to those found in human tumours by using the IARC database of TP53 mutations. The results show that the TP53 mutational patterns found in human tumours can be only partly ascribed to genotoxin exposure. A complex interplay between the functional impact of the mutations on p53 phenotype and the cancer natural history may affect these patterns. However, our results strongly support that genotoxins exposure plays a major role in the aetiology of the considered cancers. PMID:22319594

Analysis of the complete DNA sequence of the temperate bacteriophage TP901-1: Evolution, structure, and genome organization of lactococcal bacteriophages

DEFF Research Database (Denmark)

Brøndsted, Lone; Østergaard, Solvej; Pedersen, Margit

2001-01-01

A complete analysis of the entire genome of the temperate lactococcal bacteriophage TP901-1 has been performed and the function of 21 of 56 TP901-1-encoded ORFs has been assigned. This knowledge has been used to propose 10 functional modules each responsible for specific functions during...

TP53 mutation and human papilloma virus status of oral squamous cell carcinomas in young adult patients.

Science.gov (United States)

Braakhuis, B J M; Rietbergen, M M; Buijze, M; Snijders, P J F; Bloemena, E; Brakenhoff, R H; Leemans, C R

2014-09-01

Little is known about the molecular carcinogenesis of oral squamous cell carcinoma (OSCC) in young adult patients. The aim of this study was to investigate the detailed TP53 mutation and human papilloma virus (HPV) status of OSCC in patients, younger than 45 years. TP53 mutations were determined with direct sequencing on paraffin-embedded carcinoma tissue from 31 young patients and compared with two older age OSCC reference groups: one from the same institute (N = 87) and an independent one (N = 675). Biologically active tumour HPV was detected by p16-immunohistochemistry followed by a HPV-DNA GP5 + /6 + -PCR. HPV16 was present in one OSCC (3%). TP53 mutations were found in 14 (45%) OSCC: five were missense and nine resulted in a truncated protein. Six of these latter were insertions or deletions of one or more nucleotides leading to frameshift, one was at a splice site and two resulted in a stop codon. The percentage of truncating mutations (64% of all mutations) was higher than that observed in the institute's reference group (44%, P = 0.23) and in the independent reference group (24%, P = 0.002). This study shows that TP53 mutations are common in OSCC of young adult patients; infection with biologically active HPV is rare. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Influencia lunar en cultivos, animales y ser humano

OpenAIRE

Mera Andrade, Rafael; Artieda Rojas, Jorge; Muñoz Espinoza, Manolo; Romero Viamonte, Katherine

2017-01-01

Los saberes ancestrales son la base para solucionar problemas de manera sencilla. La luna, desde tiempos pasados se ha mostrado como un indicador de afectaciones tanto en el suelo, los animales de distintas regiones y el hombre. La influencia lunar tiene un gran efecto en la pecuaria, debido a que los animales dependen de la luminosidad para alimentarse o salvaguardar su vida, las fases lunares tienen un efecto positivo en la ganadería y en los animales marinos ya que benefician algunos aspec...

Molecular Characterization of TP53 Gene in Human Populations Exposed to Low-Dose Ionizing Radiation

Directory of Open Access Journals (Sweden)

Igor Brasil-Costa

2013-01-01

Full Text Available Ionizing radiation, such as that emitted by uranium, may cause mutations and consequently lead to neoplasia in human cells. The TP53 gene acts to maintain genomic integrity and constitutes an important biomarker of susceptibility. The present study investigated the main alterations observed in exons 4, 5, 6, 7, and 8 of the TP53 gene and adjacent introns in Amazonian populations exposed to radioactivity. Samples were collected from 163 individuals. Occurrence of the following alterations was observed: (i a missense exchange in exon 4 (Arg72Pro; (ii 2 synonymous exchanges, 1 in exon 5 (His179His, and another in exon 6 (Arg213Arg; (iii 4 intronic exchanges, 3 in intron 7 (C → T at position 13.436; C → T at position 13.491; T → G at position 13.511 and 1 in intron 8 (T → G at position 13.958. Alteration of codon 72 was found to be an important risk factor for cancer development (P=0.024; OR=6.48; CI: 1.29–32.64 when adjusted for age and smoking. Thus, TP53 gene may be an important biomarker for carcinogenesis susceptibility in human populations exposed to ionizing radiation.

Oropharyngeal Squamous Cell Carcinoma Treated With Radiotherapy or Radiochemotherapy: Prognostic Role of TP53 and HPV Status

International Nuclear Information System (INIS)

Fallai, Carlo; Perrone, Federica; Licitra, Lisa; Pilotti, Silvana; Locati, Laura; Bossi, Paolo; Orlandi, Ester; Palazzi, Mauro; Olmi, Patrizia

2009-01-01

Purpose: To study the prognostic value of the TP53 mutation and human papilloma virus (HPV) status in oropharyngeal squamous cell carcinoma (OPSCC). Methods and materials: The TP53 mutation and HPV status were analyzed in 78 cases of locoregionally advanced OPSCC. The possible correlation of these factors with locoregiownal control, relapse-free survival, disease-specific survival, and overall survival (OS) was also investigated. Results: Of these 78 cases, 22 had disruptive and 22 had non-disruptive (silent) TP53 mutations; the remaining 34 cases had wild-type (WT) TP53. HPV 16 DNA was found in 9 cases (11%), but all HPV-positive (HPV+) cases carried a functional p53 protein, except for 1 case that had a silent mutation. HPV+ patients fared better than HPV-negative (HPV-) patients in terms of all survival parameters, with highly statistically significant differences between the groups. Specifically, no distant metastases were observed in the HPV+ patients, whereas they occurred in 17% of the HPV- patients. However, no difference was observed between the WT TP53 and mutation group, even when this was analyzed in terms of disruptive and non-disruptive mutations. Regardless, treatment with chemotherapy nearly doubled the 5-year OS rates, both in the mutation (42% vs. 22%) and WT (30 vs. 16%) group, but only the mutation group showed improvement in all survival parameters. In addition, the second tumor-free 5-year survival rate was 72% in HPV- cases, but no second tumors were observed in HPV+ and WT p53 cases. Conclusions: Patients with HPV+ OPSCC have an excellent prognosis after radiochemotherapy, but cisplatin-based chemotherapy may not confer a satisfactory outcome, especially in WT cases, thereby justifying the additional or alternative use of taxanes and epidermal growth factor receptors inhibitors. Uncommon distant metastases and second tumors in the HPV+ group may be cause for clinicians to review the follow-up policies in these patients.

Transcriptional dissection of melanoma identifies a high-risk subtype underlying TP53 family genes and epigenome deregulation

Science.gov (United States)

Badal, Brateil; Solovyov, Alexander; Di Cecilia, Serena; Chan, Joseph Minhow; Chang, Li-Wei; Iqbal, Ramiz; Aydin, Iraz T.; Rajan, Geena S.; Chen, Chen; Abbate, Franco; Arora, Kshitij S.; Tanne, Antoine; Gruber, Stephen B.; Johnson, Timothy M.; Fullen, Douglas R.; Phelps, Robert; Bhardwaj, Nina; Bernstein, Emily; Ting, David T.; Brunner, Georg; Schadt, Eric E.; Greenbaum, Benjamin D.; Celebi, Julide Tok

2017-01-01

BACKGROUND. Melanoma is a heterogeneous malignancy. We set out to identify the molecular underpinnings of high-risk melanomas, those that are likely to progress rapidly, metastasize, and result in poor outcomes. METHODS. We examined transcriptome changes from benign states to early-, intermediate-, and late-stage tumors using a set of 78 treatment-naive melanocytic tumors consisting of primary melanomas of the skin and benign melanocytic lesions. We utilized a next-generation sequencing platform that enabled a comprehensive analysis of protein-coding and -noncoding RNA transcripts. RESULTS. Gene expression changes unequivocally discriminated between benign and malignant states, and a dual epigenetic and immune signature emerged defining this transition. To our knowledge, we discovered previously unrecognized melanoma subtypes. A high-risk primary melanoma subset was distinguished by a 122-epigenetic gene signature (“epigenetic” cluster) and TP53 family gene deregulation (TP53, TP63, and TP73). This subtype associated with poor overall survival and showed enrichment of cell cycle genes. Noncoding repetitive element transcripts (LINEs, SINEs, and ERVs) that can result in immunostimulatory signals recapitulating a state of “viral mimicry” were significantly repressed. The high-risk subtype and its poor predictive characteristics were validated in several independent cohorts. Additionally, primary melanomas distinguished by specific immune signatures (“immune” clusters) were identified. CONCLUSION. The TP53 family of genes and genes regulating the epigenetic machinery demonstrate strong prognostic and biological relevance during progression of early disease. Gene expression profiling of protein-coding and -noncoding RNA transcripts may be a better predictor for disease course in melanoma. This study outlines the transcriptional interplay of the cancer cell’s epigenome with the immune milieu with potential for future therapeutic targeting. FUNDING

BRCA1 and TP53 Gene-Mutations: Family Predisposition and Radioecological Risk of Developing Breast Cancer.

Science.gov (United States)

Apsalikov, Bakytbek; Manambaeva, Zukhra; Ospanov, Erlan; Massabayeva, Meruyert; Zhabagin, Kuantkan; Zhagiparova, Zhanar; Maximov, Vladymir; Voropaeva, Elena; Apsalikov, Kazbek; Belikhina, Tatiana; Abdrahmanov, Ramil; Cherepkova, Elena; Tanatarov, Sayat; Massadykov, Adilzhan; Urazalina, Naylia

2016-01-01

Frequencies of polymorphisms of genes BRCA1 and TP53 in breast cancer (BC) patients with a BC family history and radiation history were assessed and compared in the Semey region of Kazakhstan. The study included 60 women directly irradiated by the activities of the Semipalatinsk test site with a calculated effective equivalent dose of 500 mSv and their first generation descendants (group BC+Her+Exp); 65 women with family BC and absence of radiological history - the effective equivalent dose due to anthropogenic sources not exceeding 50 mSv (group BC+Her-Exp). The comparison group consisted of 65 women patients with breast cancer without family and radiological history (BC-Her-Exp). The control group comprised 60 women without breast cancer and without family and radiological history (nonBC). We carried out the genotyping of the polymorphisms c.2311T>C, c.4308T>C and 5382insC of the BRCA1 gene and rs1042522 of the TP53 gene. The frequency of the polymorphism c.2311T>C was significantly higher in patients of the group BC+Her+Exp than in healthy women, and of the polymorphism 5382insC in BC+Her+Exp compared to all other groups. The frequency of the rs1042522 polymorphism of TP53 was significantly higher in all groups of patients with breast cancer compared with the control group. Differences between groups of women with breast cancer were significant only in BC+Her+Exp vs. BC+Her-Exp. Combinations of polymorphisms of the genes BRCA1 and TP53 predominated in women with a family and radiological history.

Investigation of steam oxidation behaviour of TP347H FG Part 2: Exposure at 91 bar

DEFF Research Database (Denmark)

Jianmin, J; Montgomery, Melanie; Larsen, OH

2005-01-01

Tube specimens of TP347FG were exposed in a test superheater loop in a biomass plant in Denmark. The specimens were exposed to surface metal temperatures in the range of 455-568C, steam pressure of 91 bar and exposure duration of 3500 and 8700 hours. The oxide thickness and morphology was investi......Tube specimens of TP347FG were exposed in a test superheater loop in a biomass plant in Denmark. The specimens were exposed to surface metal temperatures in the range of 455-568C, steam pressure of 91 bar and exposure duration of 3500 and 8700 hours. The oxide thickness and morphology...

Biology of the temperate Streptococcus thermophilus bacteriophage TP-J34 and physical characterization of the phage genome

International Nuclear Information System (INIS)

Neve, Horst; Freudenberg, Wiebke; Diestel-Feddersen, Frederike; Ehlert, Regina; Heller, Knut J.

2003-01-01

The temperate Streptococcus thermophilus bacteriophage TP-J34 was identified in the lysogenic host strain J34. The majority of phage particles produced upon induction was defective and noninfectious, consisting of DNA-filled heads lacking tails. A physical map (45.6 kb) was established. Analysis of minor restriction bands of the DNA isolated from phage particles as well as the analysis of the protein pattern indicated that phage TP-J34 is a pac-type phage. This was confirmed by immunoelectron microscopy using antisera raised against virulent cos- and pac-type S. thermophilus phages. The lysogenic host J34 but not its noninducible derivate J34-12 contained phage DNA in the nonintegrated state and exhibited autolysis at elevated temperatures. Prophage-carrying strains grew homogeneously while 16 of 20 prophage-cured derivatives aggregated and sedimented rapidly. When phage TP-J34 was propagated lytically on a prophage-cured host strain, a 2.7-kb site-specific deletion occurred in the phage genome. This deletion was also identified in the prophage DNAs of relysogenized strains

The Tp0684 (MglB-2 Lipoprotein of Treponema pallidum: A Glucose-Binding Protein with Divergent Topology.

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Chad A Brautigam

Full Text Available Treponema pallidum, the bacterium that causes syphilis, is an obligate human parasite. As such, it must acquire energy, in the form of carbon sources, from the host. There is ample evidence that the principal source of energy for this spirochete is D-glucose acquired from its environment, likely via an ABC transporter. Further, there is genetic evidence of a D-glucose chemotaxis system in T. pallidum. Both of these processes may be dependent on a single lipidated chemoreceptor: Tp0684, also called TpMglB-2 for its sequence homology to MglB of Escherichia coli. To broaden our understanding of this potentially vital protein, we determined a 2.05-Å X-ray crystal structure of a soluble form of the recombinant protein. Like its namesake, TpMglB-2 adopts a bilobed fold that is similar to that of the ligand-binding proteins (LBPs of other ABC transporters. However, the protein has an unusual, circularly permuted topology. This feature prompted a series of biophysical studies that examined whether the protein's topological distinctiveness affected its putative chemoreceptor functions. Differential scanning fluorimetry and isothermal titration calorimetry were used to confirm that the protein bound D-glucose in a cleft between its two lobes. Additionally, analytical ultracentrifugation was employed to reveal that D-glucose binding is accompanied by a significant conformational change. TpMglB-2 thus appears to be fully functional in vitro, and given the probable central importance of the protein to T. pallidum's physiology, our results have implications for the viability and pathogenicity of this obligate human pathogen.

The Tp0684 (MglB-2) Lipoprotein of Treponema pallidum: A Glucose-Binding Protein with Divergent Topology.

Science.gov (United States)

Brautigam, Chad A; Deka, Ranjit K; Liu, Wei Z; Norgard, Michael V

2016-01-01

Treponema pallidum, the bacterium that causes syphilis, is an obligate human parasite. As such, it must acquire energy, in the form of carbon sources, from the host. There is ample evidence that the principal source of energy for this spirochete is D-glucose acquired from its environment, likely via an ABC transporter. Further, there is genetic evidence of a D-glucose chemotaxis system in T. pallidum. Both of these processes may be dependent on a single lipidated chemoreceptor: Tp0684, also called TpMglB-2 for its sequence homology to MglB of Escherichia coli. To broaden our understanding of this potentially vital protein, we determined a 2.05-Å X-ray crystal structure of a soluble form of the recombinant protein. Like its namesake, TpMglB-2 adopts a bilobed fold that is similar to that of the ligand-binding proteins (LBPs) of other ABC transporters. However, the protein has an unusual, circularly permuted topology. This feature prompted a series of biophysical studies that examined whether the protein's topological distinctiveness affected its putative chemoreceptor functions. Differential scanning fluorimetry and isothermal titration calorimetry were used to confirm that the protein bound D-glucose in a cleft between its two lobes. Additionally, analytical ultracentrifugation was employed to reveal that D-glucose binding is accompanied by a significant conformational change. TpMglB-2 thus appears to be fully functional in vitro, and given the probable central importance of the protein to T. pallidum's physiology, our results have implications for the viability and pathogenicity of this obligate human pathogen.

The prognostic values of the expression of Vimentin, TP53, and Podoplanin in patients with cervical cancer.

Science.gov (United States)

Lin, Jiaying; Lu, Jiaqi; Wang, Chao; Xue, Xiaohong

2017-01-01

Epithelial-mesenchymal transition (EMT), TP53, and Podoplanin have been implicated in the tumorigenesis and metastasis of human cancers. Nevertheless, the clinical significance of these markers in cancer patients is still not clear. In this study, we sought to determine the prognostic values of Vimentin, TP53, and Podoplanin in patients with cervical cancer. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot analysis were performed to determine the messenger RNA and protein expression levels of Vimentin, TP53, and Podoplanin, respectively, in cervical squamous cell carcinoma and adjacent normal cervical tissues. Additionally, the expression levels of Podoplanin were also measured in 130 cervical cancer patients (FIGO stages Ib1-IIa2) using immunohistochemistry (IHC) staining. The mRNA expression levels of Vimentin, TP53, and Podoplanin were considerably elevated in cervical cancer tissues, compared with those in the adjacent normal cervical tissues. Additionally, the protein expression levels of Vimentin were closely correlated with the age of onset (P = 0.007), lymph node metastasis (P = 0.007), lymphatic invasion (P = 0.024), disease recurrence (P < 0.001), and the clinical prognosis of patients with cervical cancer (P < 0.001). Our multivariate analysis also suggests that Vimentin is an independent marker for survival in cervical cancer patients. Furthermore, the expression levels of Vimentin are negatively correlated with the proliferation marker Ki67 expression. Our data show that Vimentin can serve as an independent prognostic marker for cervical cancer patients with primary surgery. Registration number ChiCTR-TRC-06000236 Registered 15 December 2006.

Peak-Temperature (Tp) estimates with Raman micro-spectroscopy on carbonaceous material (RSCM) as a tool for distinguishing tectometamorphic regimes in the Tauern Window (Eastern Alps, Austria)

Science.gov (United States)

Scharf, A.; Ziemann, M. A.; Handy, M. R.

2012-04-01

Raman micro-spectroscopy of CM in 201 samples from the eastern part of the Tauern Window reveal the overprinting of HP subduction metamorphism, post-nappe HT metamorphism and late orogenic crustal attenuation during exhumation. The following patterns of our CM data lend insight into this evolution, especially when considered in the context of the distribution of mineral parageneses, radiometric ages and structures in the Tauern Window: (1) a continuous increase in Tp (330-500°C) across nappe boundaries between two oceanic units (Valais, Piemont) in the NE part of the Tauern Window indicates that temperatures equilibrated after accretion and nappe stacking. The Tp gradient preserved in this area is ca. 10°C/km; (2) a higher Tp gradient (20-25°C/km) in the footwall of a major top-SE extensional shear zone affecting the same units at the E end of the Tauern Window reveals that the previously equilibrated Tp gradient was attenuated during doming and exhumation; (3) identical Tp estimates (500°C) -within error and for a given calibration (ref. below) - are recorded at the top and bottom of a moderately E-dipping basement nappe (Storz Nappe) within a foreland-dipping duplex (the Venediger Nappe Complex, VNC) forming the basement core of the Tauern Window. The Tp value at the top of this nappe occurs at the base of the attenuated Tp gradient described in (2), whereas the Tp at the bottom of the nappe is typical for high Tp values (530-640°C) in the core of the duplex that is exposed in a post-nappe dome (Hochalm) in the SE part of the Tauern Window. We intepret Tp values in the central part of the Tauern Window (530°C) that contain relict HP assemblages and are unaffected by doming as the maximum temperature of subduction-related metamorphism. Existing radiometric data in the area as well as from related units in other parts of the Tauern Window indicate that the thermal peak of HP metamorphism occurred at 38-40 Ma (Kurz et al. 2008, refs therein), followed by HT

DNA methylation patterns of candidate genes regulated by thymine DNA glycosylase in patients with TP53 germline mutations

Energy Technology Data Exchange (ETDEWEB)

Fortes, F.P. [CIPE, Laboratrio de Oncogentica Molecular, A.C. Camargo Cancer Center, São Paulo, SP (Brazil); Kuasne, H. [CIPE, Laboratrio NeoGene, A.C. Camargo Cancer Center, São Paulo, SP (Brazil); Departamento de Urologia, Faculdade de Medicina, Universidade Estadual Paulista, Botucatu, SP (Brazil); Marchi, F.A. [CIPE, Laboratrio NeoGene, A.C. Camargo Cancer Center, São Paulo, SP (Brazil); Programa Inter-Institucional em Bioinformtica, Instituto de Matemtica e Estatstica, Universidade So Paulo, So Paulo, SP (Brazil); Miranda, P.M. [CIPE, Laboratrio NeoGene, A.C. Camargo Cancer Center, São Paulo, SP (Brazil); Rogatto, S.R. [CIPE, Laboratrio NeoGene, A.C. Camargo Cancer Center, São Paulo, SP (Brazil); Departamento de Urologia, Faculdade de Medicina, Universidade Estadual Paulista, Botucatu, SP (Brazil); Achatz, M.I. [CIPE, Laboratrio de Oncogentica Molecular, A.C. Camargo Cancer Center, São Paulo, SP (Brazil); Departamento de Oncogentica, A.C. Camargo Cancer Center, So Paulo, SP (Brazil)

2015-04-28

Li-Fraumeni syndrome (LFS) is a rare, autosomal dominant, hereditary cancer predisposition disorder. In Brazil, the p.R337H TP53 founder mutation causes the variant form of LFS, Li-Fraumeni-like syndrome. The occurrence of cancer and age of disease onset are known to vary, even in patients carrying the same mutation, and several mechanisms such as genetic and epigenetic alterations may be involved in this variability. However, the extent of involvement of such events has not been clarified. It is well established that p53 regulates several pathways, including the thymine DNA glycosylase (TDG) pathway, which regulates the DNA methylation of several genes. This study aimed to identify the DNA methylation pattern of genes potentially related to the TDG pathway (CDKN2A, FOXA1, HOXD8, OCT4, SOX2, and SOX17) in 30 patients with germline TP53mutations, 10 patients with wild-type TP53, and 10 healthy individuals. We also evaluated TDG expression in patients with adrenocortical tumors (ADR) with and without the p.R337H TP53 mutation. Gene methylation patterns of peripheral blood DNA samples assessed by pyrosequencing revealed no significant differences between the three groups. However, increased TDG expression was observed by quantitative reverse transcription PCR in p.R337H carriers with ADR. Considering the rarity of this phenotype and the relevance of these findings, further studies using a larger sample set are necessary to confirm our results.

Hoteles y sanatorios: influencia de la tuberculosis en la arquitectura del turismo de masas

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Eduardo Jiménez Morales

Full Text Available Resumen Este artículo pretende constatar la influencia que ha ejercido la tuberculosis en la formalización de la arquitectura del turismo de masas. Para ello se repasa la evolución tipológica del hotel a partir de la eclosión del sanatorio antituberculoso en el contexto de la estación turística alpina de mediados del siglo XIX. Un recorrido que se realiza tendiendo puentes entre los progresos en arquitectura y los avances en terapias médicas. El objetivo es hacer evidente el proceso de hibridación que se produce entre ambas tipologías arquitectónicas. Una imbricación que se extiende hasta la Segunda Guerra Mundial pero cuya influencia aún persiste en la arquitectura del turismo masivo de sol y playa.

Non-Watson-Crick structures in oligodeoxynucleotides: Self-association of d(TpCpGpA) stabilized at acidic pH

International Nuclear Information System (INIS)

Topping, R.J.; Stone, M.P.; Brush, C.K.; Harris, T.M.

1988-01-01

The 1 H NMR spectrum of the tetradeoxynucleotide d(TpCpGpA) was examined as a function of temperature, pH, and concentration. At pH 7 and above the solution conformation for this oligodeoxynucleotide appears to be a mixture of random coil and Watson-Crick duplex. At 25 degree C, a pH titration of d(TpCpGaA) shown that distinct conformational changes occur as the pH is lowered below 7.0. These conformational changes are reversible upon readjusting the pH to neutrality, indicating the presence of a pH-dependent set of conformational equilibria. At 25 degree C, the various conformational state in the mixture are in rapid exchange on the NMR time scale. Examination of the titration curve shown the presence of distinct conformational states at pH greater than 7, and between pH 4 and pH 5. When the pH titration is repeated at 5 degree C, the conformational equilibria are in slow exchange on the NMR time scale; distinct signals from each conformational state are observable. The stable conformational state present between pH 4 and pH 5 represents an ordered conformation of d(TpCpGpA) which dissociates to a less ordered structure upon raising the temperature. The ordered conformation differs from the Watson-Crick helix, as is shown from nuclear Overhauser enhancement experiments, as well as chemical shift data. These results indicate that their ordered conformation is similar to the conformation of d(TpCpGpA) observed between pH 4 and pH 5. In the present case it is likely that stabilization of an ordered duplex conformation for d(TpCpGpA) is achieved by protonation of cytosine. A possible model which could explain the data involves formation of Hoogsteen C + :G base pairs

Influencia de la actividad física y la alimentación equilibrada en la mejora de la calidad de vida /

OpenAIRE

Vidaurrazaga Dobaran, Andoni

2013-01-01

Se estudia la influencia que la actividad física tiene en la calidad de vida de las personas. Es de sobra conocido que vivir de forma saludable es beneficioso para la salud pero se trata de esclarecer qué entendemos por “vivir de forma saludable” y cuál puede ser su influencia en el mantenimiento y mejora de la calidad de vida de las personas

Analysis of the collar-whisker structure of temperate lactococcal bacteriophage TP901-1

DEFF Research Database (Denmark)

Vegge, Christina Skovgaard; Neve, Horst; Brøndsted, Lone

2006-01-01

Proteins homologous to the protein NPS (neck passage structure) are widespread among lactococcal phages. We investigated the hypothesis that NPS is involved in the infection of phage TP901-1 by analysis of an NPS mutant. NPS was determined to form a collar-whisker complex but was shown to be none...

Sobre los determinantes y efectos de la influencia de politica (On the Determinants and Effects of Political Influence)

OpenAIRE

Alberto Chong; Mark Gradstein

2007-01-01

En este trabajo se emplea una encuesta de gran alcance entre empresas de varios países para evaluar su influencia en las políticas oficiales. Se halló que la influencia guarda una relación con empresas más grandes propiedad del Estado que tienen un alto grado de concentración de la propiedad. Por el contrario, la tenencia foránea tiene escasa importancia. También se descubrió que la medida en que se considera que las políticas gubernamentales y la legislación entorpecen el crecimiento de las ...

Association of polymorphism in TP53 gene with susceptibility and radiation sensitivity of non-small-cell-lung cancer in Chinese population

International Nuclear Information System (INIS)

Shao Guoguang; Liu Linlin; Xu Chuanjie

2005-01-01

Objective: To study the association of polymorphism in TP53 gene with the susceptibility and radiation sensitivity of non-small-cell-lung cancer (NSCLC) of the population in the North of China. Methods: Using RFLP-PCR assays, TP53 genotypes were detected by amplifying DNA fragments with sequence specific primers and digested by FnuD II enzyme in 88 patients with NSCLC as well as 112 healthy controls. Results: The C/C allele frequency was significantly higher in NSCLC patients than that in the healthy controls (χ 2 =5.65, P=0.017). The C/C genotype frequency was significantly higher in NSCLC patients than that of the healthy controls (χ 2 =9.33, P=0.0023). The risk of C/C homozygotes in NSCLC patients was about 2.7 times against G/G homozygotes with odds ratio of 2.43(95% CI=1.32-4.51). Conclusion: In the population in the North of China, TP53 C/C genotype is closely associated with the susceptibility of NSCLC. There is no significant relationship between the polymorphism in TP53 gene and radiation sensitivity in NSCLC. (authors)

Frequency of Somatic TP53 Mutations in Combination with Known Pathogenic Mutations in Colon Adenocarcinoma, Non–Small Cell Lung Carcinoma, and Gliomas as Identified by Next-Generation Sequencing

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Zahra Shajani-Yi

2018-03-01

Full Text Available The tumor suppressor gene TP53 is the most frequently mutated gene in human cancer. It encodes p53, a DNA-binding transcription factor that regulates multiple genes involved in DNA repair, metabolism, cell cycle arrest, apoptosis, and senescence. TP53 is associated with human cancer by mutations that lead to a loss of wild-type p53 function as well as mutations that confer alternate oncogenic functions that enable them to promote invasion, metastasis, proliferation, and cell survival. Identifying the discrete TP53 mutations in tumor cells may help direct therapies that are more effective. In this study, we identified the frequency of individual TP53 mutations in patients with colon adenocarcinoma (48%, non–small cell lung carcinoma (NSCLC (36%, and glioma/glioblastoma (28% at our institution using next-generation sequencing. We also identified the occurrence of somatic mutations in numerous actionable genes including BRAF, EGFR, KRAS, IDH1, and PIK3CA that occurred concurrently with these TP53 mutations. Of the 480 tumors examined that contained one or more mutations in the TP53 gene, 219 were colon adenocarcinomas, 215 were NSCLCs, and 46 were gliomas/glioblastomas. Among the patients positive for TP53 mutations diagnosed with colon adenocarcinoma, 50% also showed at least one mutation in pathogenic genes of which 14% were BRAF, 33% were KRAS, and 3% were NRAS. Forty-seven percent of NSCLC patients harboring TP53 mutations also had a mutation in at least one actionable pathogenic variant with the following frequencies: BRAF: 4%, EGFR: 10%, KRAS: 28%, and PIK3CA: 4%. Fifty-two percent of patients diagnosed with glioma/glioblastoma with a positive TP53 mutation had at least one concurrent mutation in a known pathogenic gene of which 9% were CDKN2A, 41% were IDH1, and 11% were PIK3CA.

The association of TP53 mutations with the resistance of colorectal carcinoma to the insulin-like growth factor-1 receptor inhibitor picropodophyllin

International Nuclear Information System (INIS)

Wang, Quan; Wei, Feng; Lv, Guoyue; Li, Chunsheng; Liu, Tongjun; Hadjipanayis, Costas G; Zhang, Guikai; Hao, Chunhai; Bellail, Anita C

2013-01-01

There is growing evidence indicating the insulin-like growth factor 1 receptor (IGF-1R) plays a critical role in the progression of human colorectal carcinomas. IGF-1R is an attractive drug target for the treatment of colon cancer. Picropodophyllin (PPP), of the cyclolignan family, has recently been identified as an IGF-1R inhibitor. The aim of this study is to determine the therapeutic response and mechanism after colorectal carcinoma treatment with PPP. Seven colorectal carcinoma cell lines were treated with PPP. Following treatment, cells were analyzed for growth by a cell viability assay, sub-G1 apoptosis by flow cytometry, caspase cleavage and activation of AKT and extracellular signal-regulated kinase (ERK) by western blot analysis. To examine the in vivo therapeutic efficacy of PPP, mice implanted with human colorectal carcinoma xenografts underwent PPP treatment. PPP treatment blocked the phosphorylation of IGF-1R, AKT and ERK and inhibited the growth of TP53 wild-type but not mutated colorectal carcinoma cell lines. The treatment of PPP also induced apoptosis in TP53 wild-type cells as evident by the presence of sub-G1 cells and the cleavage of caspase-9, caspase-3, DNA fragmentation factor-45 (DFF45), poly (ADP-ribose) polymerase (PARP), and X-linked inhibitor of apoptosis protein (XIAP). The loss of BAD phosphorylation in the PPP-treated TP53 wild type cells further suggested that the treatment induced apoptosis through the BAD-mediated mitochondrial pathway. In contrast, PPP treatment failed to induce the phosphorylation of AKT and ERK and caspase cleavage in TP53 mutated colorectal carcinoma cell lines. Finally, PPP treatment suppressed the growth of xenografts derived from TP53 wild type but not mutated colorectal carcinoma cells. We report the association of TP53 mutations with the resistance of treatment of colorectal carcinoma cells in culture and in a xenograft mouse model with the IGF-1R inhibitor PPP. TP53 mutations often occur in colorectal

La polémica influencia de Adolfo Posada en el pensamiento de los catedráticos de derecho de la generación del 900

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Raquel García Bouzas

2014-10-01

Full Text Available Contenido: Las razones de la influencia de Posada entre los juristas rioplatenses. Un particular enfoque del krausismo. Receptividad y difusión de su pensamiento. Concepción organicista del estado. El sufragio como un deber.Valoración de su influencia en nuestra historia intelectual

Identification of colorectal cancer patients with tumors carrying the TP53 mutation on the codon 72 proline allele that benefited most from 5-fluorouracil (5-FU) based postoperative chemotherapy

International Nuclear Information System (INIS)

Godai, Ten-i; Sakuma, Yuji; Tsuchiya, Eiju; Kameda, Yoichi; Akaike, Makoto; Miyagi, Yohei; Suda, Tetsuji; Sugano, Nobuhiro; Tsuchida, Kazuhito; Shiozawa, Manabu; Sekiguchi, Hironobu; Sekiyama, Akiko; Yoshihara, Mitsuyo; Matsukuma, Shoichi

2009-01-01

Although postoperative chemotherapy is widely accepted as the standard modality for Dukes' stage C or earlier stage colorectal cancer (CRC) patients, biomarkers to predict those who may benefit from the therapy have not been identified. Previous in vitro and clinical investigations reported that CRC patients with wild-type p53 gene (TP53)-tumors benefit from 5-fluorouracil (5-FU) based chemotherapy, while those with mutated TP53-tumors do not. However, these studies evaluated the mutation-status of TP53 by immunohistochemistry with or without single-strand conformation polymorphism, and the mutation frequency was different from study to study. In addition, the polymorphic status at p53 codon 72, which results in arginine or proline residues (R72P) and is thought to influence the function of the protein significantly, was not examined. To evaluate the significance of the TP53 mutation as a molecular marker to predict the prognosis of CRC patients, especially those who received postoperative chemotherapy, we examined the mutation by direct sequencing from fresh CRC tumors and evaluated the R72P polymorphism of the mutated TP53 by a combined mutant allele- and polymorphic allele-specific polymerase chain reaction (PCR). The TP53 mutation occurred in 147 (70%) of 211 Japanese CRC tumors. The mutation was observed in 93 (63%) tumors on the R72 allele and in 54 (37%) tumors on the P72 allele. Although the alterations to TP53 have no prognostic significance for CRC patients overall, we found that Dukes' stage C CRC patients who did not receive postoperative chemotherapy and carried the mutated TP53-R72 showed significantly longer survival times than those with the mutated TP53-P72 when evaluated by overall survival (p = 0.012). Using a combined mutant allele- and polymorphic allele-specific PCR, we defined the codon 72 polymorphic status of the TP53 mutated allele in Japanese CRC patients. We raised a possibility that Dukes' stage C colorectal cancer

MicroRNA‑663b mediates TAM resistance in breast cancer by modulating TP73 expression.

Science.gov (United States)

Jiang, Hua; Cheng, Lin; Hu, Pan; Liu, Renbin

2018-05-23

Breast cancer is the second leading cause of cancer‑associated mortalities in women. Tamoxifen (TAM) is an endocrine therapy commonly used in the treatment of patients with breast cancer expressing estrogen receptor α. However, treatment often ends in failure due to the emergence of drug resistance. MicroRNAs (miRNAs), a family of small non‑coding RNAs, serve critical roles in the regulation of gene expression and cell events. To date, whether miRNA‑663b could mediate TAM resistance in breast cancer remains unknown. Therefore, the aim of the present study was to investigate the role of miRNA‑663b in TAM resistance in breast cancer. The results demonstrated that miRNA‑663b was upregulated in breast cancer with TAM resistance. Tumor protein 73 (TP73) was a direct target of miRNA‑663b, and was negatively regulated by miRNA‑663b in MCF‑7 cells. Furthermore, it was identified that downregulation of miRNA‑663b inhibited cell proliferation ability and promoted cell apoptosis, resulting in enhanced TAM sensitivity. In addition, these findings suggested that TP73 silencing may have eliminated the effects of miRNA‑663b inhibitor on breast cancer cells. In conclusion, the present study verified a novel molecular link between miRNA‑663b and TP73, and indicated that miRNA‑663b may be a critical therapeutic target in breast cancer.

Influencia de la preparación física en el dominio técnico futbolista 12-14 años del Club el Nacional

OpenAIRE

Alomoto Navarrete, Rocío

2014-01-01

Siendo conocedores de la realidad deportiva ecuatoriana en la formación de deportistas en diferentes etápas sabiendo que en la étapa de inciación deportiva el aprendizaje de la técnica deportiva depende de una buena preparación física es importante analizar la influencia de la preparación física en el dominio técnico de futbolista 12-14 años así como su influencia en el dominio técnico de este deporte. El objetivo es proponer una programa de ejercicios para la influencia de la preparación fís...

THE CONTRIBUTION OF TP-AGB STARS TO THE MID-INFRARED COLORS OF NEARBY GALAXIES

International Nuclear Information System (INIS)

Chisari, Nora E.; Kelson, Daniel D.

2012-01-01

We study the mid-infrared color space of 30 galaxies from the Spitzer Infrared Nearby Galaxies Survey (SINGS) survey for which Sloan Digital Sky Survey data are also available. We construct two-color maps for each galaxy and compare them to results obtained from combining Maraston evolutionary synthesis models, galactic thermally pulsating asymptotic giant branch (TP-AGB) colors, and smooth star formation histories. For most of the SINGS sample, the spatially extended mid-IR emission seen by Spitzer in normal galaxies is consistent with our simple model in which circumstellar dust from TP-AGB stars dominates at 8 and 24 μm. There is a handful of exceptions that we identify as galaxies that have high star formation rates presumably with star formation histories that cannot be assumed to be smooth, or anemic galaxies, which were depleted of their H I at some point during their evolution and have very low ongoing star formation rates.

THE CONTRIBUTION OF TP-AGB STARS TO THE MID-INFRARED COLORS OF NEARBY GALAXIES

Energy Technology Data Exchange (ETDEWEB)

Chisari, Nora E. [Department of Astrophysical Sciences, Princeton University, 4 Ivy Lane, Princeton, NJ 08544 (United States); Kelson, Daniel D., E-mail: nchisari@astro.princeton.edu [Observatories of the Carnegie Institution of Science, 813 Santa Barbara St., Pasadena, CA 91101 (United States)

2012-07-10

We study the mid-infrared color space of 30 galaxies from the Spitzer Infrared Nearby Galaxies Survey (SINGS) survey for which Sloan Digital Sky Survey data are also available. We construct two-color maps for each galaxy and compare them to results obtained from combining Maraston evolutionary synthesis models, galactic thermally pulsating asymptotic giant branch (TP-AGB) colors, and smooth star formation histories. For most of the SINGS sample, the spatially extended mid-IR emission seen by Spitzer in normal galaxies is consistent with our simple model in which circumstellar dust from TP-AGB stars dominates at 8 and 24 {mu}m. There is a handful of exceptions that we identify as galaxies that have high star formation rates presumably with star formation histories that cannot be assumed to be smooth, or anemic galaxies, which were depleted of their H I at some point during their evolution and have very low ongoing star formation rates.

Effects of external stresses on hot corrosion behavior of stainless steel TP347HFG

International Nuclear Information System (INIS)

Fu, Jiapeng; Zhou, Qulan; Li, Na; Liu, Zhuhan; Liu, Taisheng

2016-01-01

Highlights: • Hot corrosion tests of TP347HFG under different stresses were conducted. • The corrosion resistance was strengthened by the exertion of tensile stresses. • External stresses promoted faster formation of the protective Cr_2O_3 layer. • Specimens under critical stress 40 MPa condition present the best resistance. - Abstract: Hot corrosion experiments of alloy TP347HFG under different stresses were conducted. Corroded specimens were examined by means of corrosion products, morphology and compositional changes in corrosion scales. The corrosion behavior was strongly associated with the formation of oxides layers. The corrosion resistance was strengthened by the external stress. It seemed that the exertion of stresses caused many micro cracks and defects, which acted as faster and easier diffusion paths for Cr atoms to diffuse to the surface, and thus, promote faster formation of the protective Cr_2O_3 oxide layer. Critical stress 40 MPa was found, specimens under which present the best resistance.

Influencia del tempo de la música en las emociones

Directory of Open Access Journals (Sweden)

Jonathan Azael Caballero-Meneses

2010-01-01

exposición de 24 estudiantes de licenciatura a treinta extractos musicales de distinto tempo. Además se evaluó la posible modulación de la relación tempo-valencia por el rasgo de personalidad extroversión/introversión. Los resultados confirmaron la influencia del tempo sobre el arousal, pero no sobre la valencia, así como una posible mediación de la dimensión de personalidad introversión-extroversión sobre la valencia.

La influencia de la musicoterapia en ni??os con disfemia

OpenAIRE

P??rez Bazoco, M??nica

2015-01-01

En este trabajo se pretende realizar una peque??a investigaci??n sobre la influencia que tiene la musicoterapia en los ni??os que presentan disfemia. La musicoterapia es un medio de rehabilitaci??n que incide tanto desde el punto de vista fisiol??gico (como puede ser la ejercitaci??n de la voz), como desde la perspectiva emocional e intelectual, proporcionando un desarrollo afectivo y cognoscitivo. Por tanto, la din??mica planteada de modelos mel??dicos y r??tmicos parece repercutir en el des...

Association of TP53 and MDM2 polymorphisms with survival in bladder cancer patients treated with chemoradiotherapy

International Nuclear Information System (INIS)

Shinohara, Asano; Sakano, Shigeru; Hinoda, Yuji; Nishijima, Jun; Kawai, Yoshihisa; Misumi, Taku; Nagao, Kazuhiro; Hara, Takahiko; Matsuyama, Hideyasu

2009-01-01

Platinum-based chemoradiotherapy (CRT) as bladder conservation therapy has shown promising results for muscle-invasive bladder cancer. However, CRT might diminish survival as a result of the delay in cystectomy for some patients with non-responding bladder tumors. Because the p53 tumor suppression pathway, including its MDM2 counterpart, is important in chemotherapy- and radiotherapy-associated effects, functional polymorphisms in the TP53 and MDM2 genes could influence the response to treatment and the prognosis following CRT. We investigated associations between two such polymorphisms, and p53 overexpression, and response or survival in bladder cancer patients treated with CRT. The study group comprised 96 patients who underwent CRT for transitional cell carcinoma of the bladder. Single nucleotide polymorphisms (SNPs) in TP53 (codon 72, arginine>proline) and MDM2 (SNP3O9, T>G) were genotyped using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP), and nuclear expression levels of p53 were examined using immunohistochemistry. None of the genotypes or p53 overexpression was significantly associated with response to CRT. However, patients with MDM2 T/G+G/G genotypes had improved cancer-specific survival rates after CRT (P=0.009). In multivariate analysis, the MDM2 T/G+G/G genotypes, and more than two of total variant alleles in TP53 and MDM2, were independently associated with improved cancer-specific survival (P=0.031 and P=0.015, respectively). In addition, MDM2 genotypes were significantly associated with cystectomy-free survival (P=0.030). These results suggest that the TP53 and MDM2 genotypes might be useful prognostic factors following CRT in bladder cancer, helping patient selection for bladder conservation therapy. (author)

Src kinases in chondrosarcoma chemoresistance and migration: dasatinib sensitises to doxorubicin in TP53 mutant cells

Science.gov (United States)

van Oosterwijk, J G; van Ruler, M A J H; Briaire-de Bruijn, I H; Herpers, B; Gelderblom, H; van de Water, B; Bovée, J V M G

2013-01-01

Background: Chondrosarcomas are malignant cartilage-forming tumours of bone. Because of their resistance to conventional chemotherapy and radiotherapy, currently no treatment strategies exist for unresectable and metastatic chondrosarcoma. Previously, PI3K/AKT/GSK3β and Src kinase pathways were shown to be activated in chondrosarcoma cell lines. Our aim was to investigate the role of these kinases in chemoresistance and migration in chondrosarcoma in relation to TP53 mutation status. Methods: We used five conventional and three dedifferentiated chondrosarcoma cell lines and investigated the effect of PI3K/AKT/GSK3β pathway inhibition (enzastaurin) and Src pathway inhibition (dasatinib) in chemoresistance using WST assay and live cell imaging with AnnexinV staining. Immunohistochemistry on tissue microarrays (TMAs) containing 157 cartilaginous tumours was performed for Src family members. Migration assays were performed with the RTCA xCelligence System. Results: Src inhibition was found to overcome chemoresistance, to induce apoptosis and to inhibit migration. Cell lines with TP53 mutations responded better to combination therapy than wild-type cell lines (P=0.002). Tissue microarray immunohistochemistry confirmed active Src (pSrc) signalling, with Fyn being most abundantly expressed (76.1%). Conclusion: These results strongly indicate Src family kinases, in particular Fyn, as a potential target for the treatment of inoperable and metastatic chondrosarcomas, and to sensitise for doxorubicin especially in the presence of TP53 mutations. PMID:23922104

Temperate phages TP901-1 and phi LC3, belonging to the P335 species, apparently use different pathways for DNA injection in Lactococcus lactis subsp cremoris 3107

DEFF Research Database (Denmark)

Breum, Solvej Østergaard; Neve, Horst; Heller, Knut J.

2007-01-01

Five mutants of Lactococcus lactis subsp. cremoris 3107 resistant to phage TP901-1 were obtained after treatment with ethyl methanesulfonate. Two of the mutants were also resistant to phage phi LC3. The remaining three mutants were as sensitive as 3107. Mutants E46 and E100 did not adsorb the two...... phages. Mutants E119, E121 and E126 adsorbed phage phi LC3 as well as 3107 but phage TP901-1 with significantly reduced efficiency. All, except E46, could be lysogenized with phage TP901-BC1034, a derivative of TP901-1 harboring an erythromycin-resistance marker. However, the lysogenization frequency......-1. As such impairment was not seen when infecting E119, E121 and E126 with phi LC3, we conclude that TP901-1 and phi LC3 either are differently triggered by their receptor or utilize different pathways of injection....

Human TP53 polymorphism (rs1042522) modelled in mouse does not affect glucose metabolism and body composition.

Science.gov (United States)

Reiling, Erwin; Speksnijder, Ewoud N; Pronk, Amanda C M; van den Berg, Sjoerd A A; Neggers, Silvia J W; Rietbroek, Ilma; van Steeg, Harry; Dollé, Martijn E T

2014-02-13

Variation in TP53 has been associated with cancer. The pro-allele of a TP53 polymorphism in codon 72 (rs1042522) has been associated with longevity. Recently, we showed that the same allele might be involved in preservation of glucose metabolism, body composition and blood pressure during ageing. Here, we assessed glucose tolerance and body composition in mice carrying the human polymorphism. Our data do not support the previous findings in humans, suggesting that this polymorphism does not play a major role in development of glucose metabolism and body composition during ageing. Alternatively, the mouse model may not be suitable to validate these rs1042522-associated traits up to the age tested.

Major prognostic value of complex karyotype in addition to TP53 and IGHV mutational status in first-line chronic lymphocytic leukemia.

Science.gov (United States)

Le Bris, Yannick; Struski, Stéphanie; Guièze, Romain; Rouvellat, Caroline; Prade, Naïs; Troussard, Xavier; Tournilhac, Olivier; Béné, Marie C; Delabesse, Eric; Ysebaert, Loïc

2017-12-01

Chronic lymphocytic leukemia (CLL) is a lymphoproliferative disorder of remarkable heterogeneity as demonstrated by cytogenetics and molecular analyses. Complex karyotype (CK), TP53 deletions and/or mutations (TP53 disruption), IGVH mutational status, and, more recently, recurrent somatic mutations have been identified as prognostic markers in CLL. On a cohort of 110 patients with CLL treated with first-line fludarabin, cyclophosphamide, and rituximab treatment compared with 33 untreated (watch and wait) patients with CLL, we report more frequent complex karyotypes (34 vs 15%; P = .05), unmutated IGHV (70 vs 21%; P < .0001), ATM deletion (25 vs 6%, P = .02), and NOTCH mutation (3 vs 17%, P = .04). Among treated patients, 39 relapsed during the follow-up period. These patients were characterized before treatment by a higher incidence of trisomy 12 (38 vs 11%, P < .001) and TP53 disruption (31 vs 4%, P = .0002). A significantly shorter 5-year overall survival was found for treated patients with CK (72.4 vs 85.8%; P = .007), unmutated IGHV (70 vs 100%; P = .04), or TP53 disruption (55.7 vs 82.7%; P < .0001). Three risk groups were defined based on the status of TP53 disruption or unmutated IGVH, which differed significantly in terms of 5-year overall survival. Moreover, the presence of CK impacted pejoratively 5-year overall survival and progression-free survival in all these 3 groups. Conventional karyotyping therefore appears to be of value, CK being an additional factor, undetectable in classical FISH, in patients with CLL at the stage when therapy becomes required. Copyright © 2016 John Wiley & Sons, Ltd.

Importance of TP53 and RB in the repair of potentially lethal damage and induction of color junctions after exposure to ionizing radiation

NARCIS (Netherlands)

Franken, N. A. P.; van Bree, C.; ten Cate, R.; van Oven, C. H.; Haveman, J.

2002-01-01

Repair of potentially lethal damage (PLD) was investigated in cells with functional G(1)-phase arrest with wild-type TP53 and wild-type RB and in cells in which G(1)-phase arrest was abrogated by inactivation of TP53 or RB. Confluent cultures of cells were plated for clonogenic survival assay either

The Neuronal Ischemic Tolerance Is Conditioned by the Tp53 Arg72Pro Polymorphism.

Science.gov (United States)

Ramos-Araque, Maria E; Rodriguez, Cristina; Vecino, Rebeca; Cortijo Garcia, Elisa; de Lera Alfonso, Mercedes; Sanchez Barba, Mercedes; Colàs-Campàs, Laura; Purroy, Francisco; Arenillas, Juan F; Almeida, Angeles; Delgado-Esteban, Maria

2018-04-23

Cerebral preconditioning (PC) confers endogenous brain protection after stroke. Ischemic stroke patients with a prior transient ischemic attack (TIA) may potentially be in a preconditioned state. Although PC has been associated with the activation of pro-survival signals, the mechanism by which preconditioning confers neuroprotection is not yet fully clarified. Recently, we have described that PC-mediated neuroprotection against ischemic insult is promoted by p53 destabilization, which is mediated by its main regulator MDM2. Moreover, we have previously described that the human Tp53 Arg72Pro single nucleotide polymorphism (SNP) controls susceptibility to ischemia-induced neuronal apoptosis and governs the functional outcome of patients after stroke. Here, we studied the contribution of the human Tp53 Arg72Pro SNP on PC-induced neuroprotection after ischemia. Our results showed that cortical neurons expressing the Pro72-p53 variant exhibited higher PC-mediated neuroprotection as compared with Arg72-p53 neurons. PC prevented ischemia-induced nuclear and cytosolic p53 stabilization in Pro72-p53 neurons. However, PC failed to prevent mitochondrial p53 stabilization, which occurs in Arg72-p53 neurons after ischemia. Furthermore, PC promoted neuroprotection against ischemia by controlling the p53/active caspase-3 pathway in Pro72-p53, but not in Arg72-p53 neurons. Finally, we found that good prognosis associated to TIA within 1 month prior to ischemic stroke was restricted to patients harboring the Pro72 allele. Our findings demonstrate that the Tp53 Arg72Pro SNP controls PC-promoted neuroprotection against a subsequent ischemic insult by modulating mitochondrial p53 stabilization and then modulates TIA-induced ischemic tolerance.

Estudio de la influencia del lavado y del ensimaje sobre la estabilidad de hilatura y las propiedades de los hilados.

OpenAIRE

Coll Tortosa, Liberto; Palet Alsina, Daniel; Cegarra Sánchez, José; Gacén Guillén, Joaquín; Naik, Arun

1995-01-01

En el presente trabajo se estudia la influencia de un lavado deficiente de la lana sobre la estabilidad de hilatura y las propiedades de los hilados. El motivo de este estudio es solucionar el problema de la acumulación de residuos no hilables en los órganos de la máquina de hilar, que repercuten negativamente en la hilatura y en las propiedades de los hilados. Este problema fue abordado en un trabajo anterior (julio 91) en el que se analizó la influencia de la intensidad del aclarado, sin en...

Human Papillomavirus 16 Infection and TP53 Mutation: Two Distinct Pathogeneses for Oropharyngeal Squamous Cell Carcinoma in an Eastern Chinese Population

OpenAIRE

Wang, Zhen; Xia, Rong-Hui; Ye, Dong-Xia; Li, Jiang

2016-01-01

Objectives To investigate the clinicopathological characteristics, human papillomavirus (HPV) infection, p53 expression, and TP53 mutations in oropharyngeal squamous cell carcinoma (OPSCC) and determine their utility as prognostic predictors in a primarily eastern Chinese population. Methods The HPV infection status was tested via p16INK4A immunohistochemistry and validated using PCR, reverse blot hybridization and in situ hybridization (ISH) in 188 OPSCC samples. p53 expression levels and TP...

Identification of DNA-binding sites for the activator involved in late transcription of the temperate lactococcal phage TP901-1

DEFF Research Database (Denmark)

Pedersen, Margit; Kilstrup, Mogens; Hammer, Karin

2006-01-01

Alt, encoded by the lactococcal phage TP901-1, is needed for late transcription. We identify Alt as a DNA-binding protein, and footprint analysis shows that Alt binds to a region containing four imperfect direct repeats (ALT boxes) located -76 to -32 relative to the P-late transcriptional start...... site. The importance of the ALT boxes was confirmed by deletion of one or two ALT boxes and by introducing mutations in ALT boxes 1 and 4. Alt is proposed to act as a tetramer or higher multimer activating transcription of TP901-1 late genes by binding to the four ALT boxes, and bending of the DNA may...... be important for transcriptional activation of P-late. Furthermore, our results suggest that DNA replication may be required for late transcription in TP901-1. Additionally, we identify gp28 of the related lactococcal phage Tuc2009 as an activator and show that the activators required for late transcription...

TP53 germline mutation testing in 180 families suspected of Li-Fraumeni syndrome: mutation detection rate and relative frequency of cancers in different familial phenotypes

NARCIS (Netherlands)

Ruijs, M.W.G.; Verhoef, S.; Rookus, M.A.; Pruntel, R.; van der Hout, A.H.; Hogervorst, F.B.L.; Kluijt, I.; Sijmons, R.H.; Aalfs, C.M.; Wagner, A.; Ausems, M.G.E.M.; Hoogerbrugge, N.; van Asperen, C.J.; Gómez García, E.B.; Meijers-Heijboer, H.; ten Kate, L.P.; Menko, F.H.; van 't Veer, L.J.

2010-01-01

Background Li-Fraumeni syndrome (LFS) is a rare autosomal dominant cancer predisposition syndrome. Most families fulfilling the classical diagnostic criteria harbour TP53 germline mutations. However, TP53 germline mutations may also occur in less obvious phenotypes. As a result, different criteria

Association of the germline TP53 R72P and MDM2 SNP309 variants with breast cancer survival in specific breast tumor subgroups

NARCIS (Netherlands)

van den Broek, Alexandra J.; Broeks, Annegien; Horlings, Hugo M.; Canisius, Sander V. M.; Braaf, Linde M.; Langerød, Anita; van't Veer, Laura J.; Schmidt, Marjanka K.

2011-01-01

The tumor suppressor gene TP53 and its regulator MDM2 are both important players in the DNA-damage repair "TP53 response pathway". Common germline polymorphisms in these genes may affect outcome in patients with tumors characterized by additional somatic changes in the same or a related pathway. To

Identical TP53 mutations in pelvic carcinosarcomas and associated serous tubal intraepithelial carcinomas provide evidence of their clonal relationship.

Science.gov (United States)

Ardighieri, Laura; Mori, Luigi; Conzadori, Sara; Bugatti, Mattia; Falchetti, Marcella; Donzelli, Carla Maria; Ravaggi, Antonella; Odicino, Franco E; Facchetti, Fabio

2016-07-01

Pelvic carcinosarcomas (PCSs) are rare aggressive biphasic tumors that localize in the ovary, fallopian tube, or peritoneum and present frequently as bilateral disease. We undertook a morphological, p53 immunohistochemical and TP53 gene mutational analysis study in a single institution cohort of 16 PCSs in order to investigate the nature of bilateral tumors and to shed light on their origin and pathogenesis. Of the 16 patients, 10 presented with bilateral disease, 6 with a carcinosarcoma in both adnexa, and the remaining cases with a carcinosarcoma in one adnexum and a carcinoma in the opposite. The carcinoma component showed high-grade serous features in 13/16 of cases (81 %). In 10 patients (63 %), a serous tubal intraepithelial carcinoma (STIC) was found, in one case bilateral, making a total of 11 STICs. STIC was found only in cases with a carcinoma component with high-grade serous features. All 10 bilateral tumors and all 11 PCS-associated STICs showed a similar p53 immunostaining pattern. At mutation analysis of the TP53 gene, all five bilateral PCS contained an identical mutation in both localizations. Furthermore, a TP53 mutation was found in 8 of 10 STICs, with an identical mutation in the associated PCS. The finding of similar p53 immunostaining in all bilateral cases and identical TP53 mutations in most PCS-associated STIC provides evidence for a clonal relation between these neoplastic lesions, supporting a metastatic nature of bilateral PCS and suggesting that they have an extraovarian origin in a STIC.

TP53, STK11 and EGFR Mutations Predict Tumor Immune Profile and the Response to anti-PD-1 in Lung Adenocarcinoma.

Science.gov (United States)

Biton, Jerome; Mansuet-Lupo, Audrey; Pécuchet, Nicolas; Alifano, Marco; Ouakrim, Hanane; Arrondeau, Jennifer; Boudou-Rouquette, Pascaline; Goldwasser, Francois; Leroy, Karen; Goc, Jeremy; Wislez, Marie; Germain, Claire; Laurent-Puig, Pierre; Dieu-Nosjean, Marie-Caroline; Cremer, Isabelle; Herbst, Ronald; Blons, Hélène F; Damotte, Diane

2018-05-15

By unlocking anti-tumor immunity, antibodies targeting programmed cell death 1 (PD-1) exhibit impressive clinical results in non-small cell lung cancer, underlining the strong interactions between tumor and immune cells. However, factors that can robustly predict long-lasting responses are still needed. We performed in depth immune profiling of lung adenocarcinoma using an integrative analysis based on immunohistochemistry, flow-cytometry and transcriptomic data. Tumor mutational status was investigated using next-generation sequencing. The response to PD-1 blockers was analyzed from a prospective cohort according to tumor mutational profiles and to PD-L1 expression, and a public clinical database was used to validate the results obtained. We showed that distinct combinations of STK11 , EGFR and TP53 mutations, were major determinants of the tumor immune profile (TIP) and of the expression of PD-L1 by malignant cells. Indeed, the presence of TP53 mutations without co-occurring STK11 or EGFR alterations ( TP53 -mut/ STK11 - EGFR -WT), independently of KRAS mutations, identified the group of tumors with the highest CD8 T cell density and PD-L1 expression. In this tumor subtype, pathways related to T cell chemotaxis, immune cell cytotoxicity, and antigen processing were up-regulated. Finally, a prolonged progression-free survival (PFS: HR=0.32; 95% CI, 0.16-0.63, p <0.001) was observed in anti-PD-1 treated patients harboring TP53 -mut/ STK11 - EGFR -WT tumors. This clinical benefit was even more remarkable in patients with associated strong PD-L1 expression. Our study reveals that different combinations of TP53 , EGFR and STK11 mutations , together with PD-L1 expression by tumor cells, represent robust parameters to identify best responders to PD-1 blockade. Copyright ©2018, American Association for Cancer Research.

TP-Space RRT – Kinematic Path Planning of Non-Holonomic Any-Shape Vehicles

Directory of Open Access Journals (Sweden)

Jose Luis Blanco

2015-05-01

Full Text Available The autonomous navigation of vehicles typically combines two kinds of methods: a path is first planned, and then the robot is driven by a local obstacle-avoidance controller. The present work, which focuses on path planning, proposes an extension to the well-known rapidly-exploring random tree (RRT algorithm to allow its integration with a trajectory parameter-space (TP-space as an efficient method to detect collision-free, kinematically-feasible paths for arbitrarily-shaped vehicles. In contrast to original RRT, this proposal generates navigation trees, with poses as nodes, whose edges are all kinematically-feasible paths, suitable to being accurately followed by vehicles driven by pure reactive algorithms. Initial experiments demonstrate the suitability of the method with an Ackermann-steering vehicle model whose severe kinematic constraints cannot be obviated. An important result that sets this work apart from previous research is the finding that employing several families of potential trajectories to expand the tree, which can be done efficiently under the TP-space formalism, improves the optimality of the planned trajectories. A reference C++ implementation has been released as open-source.

La influencia católica en la configuración del sistema político y educativo nacional argentino a fines del siglo XIX

Directory of Open Access Journals (Sweden)

Oscar Daniel Duarte

2014-05-01

Full Text Available El presente artículo describe las diferentes corrientes de pensamiento que influenciaron en el proceso de conformación estatal argentino. Sobresalen entre ellas la línea liberal y la línea católica de fuerte influencia en la elite dirigente del país. A partir de allí realizamos un análisis sobre la orientación política tomada por el Estado y la influencia impelida por el catolicismo particularmente en el debate sobre la conformación del aparato educativo nacional. La conclusión a la que arribamos en este estudio explica el por qué del sometimiento político e ideológico al que se vio sujeto el Estado argentino respecto a la influencia del Vaticano.

Astigmatismo interno : epidemiología, clasificación e influencia en los resultados de la cirugía refractiva corneal

OpenAIRE

Gargallo Martínez, Beatriz

2015-01-01

Objetivos: Trabajos previos muestran que la corrección astigmática mediante cirugía láser en sujetos con grandes cantidades de astigmatismo interno (AI) es menos predecible. La influencia exacta del AI en los resultados de la cirugía refractiva, así como la exactitud en su medida o su relación con el resto de estructuras ópticas del ojo se desconoce. Ésta tesis pretende estudiar y analizar la epidemiología del AI y su influencia en los resultados de la cirugía refractiva. Si aumentamos el ...

TP53 mutations in ovarian carcinomas from sporadic cases and carriers of two distinct BRCA1 founder mutations; relation to age at diagnosis and survival

International Nuclear Information System (INIS)

Kringen, Pedro; Wang, Yun; Dumeaux, Vanessa; Kristensen, Gunnar; Borresen-Dale, Anne-Lise; Dorum, Anne

2005-01-01

Ovarian carcinomas from 30 BRCA1 germ-line carriers of two distinct high penetrant founder mutations, 20 carrying the 1675delA and 10 the 1135insA, and 100 sporadic cases were characterized for somatic mutations in the TP53 gene. We analyzed differences in relation to BRCA1 germline status, TP53 status, survival and age at diagnosis, as previous studies have not been conclusive. DNA was extracted from paraffin embedded formalin fixed tissues for the familial cases, and from fresh frozen specimen from the sporadic cases. All cases were treated at our hospital according to protocol. Mutation analyses of exon 2 – 11 were performed using TTGE, followed by sequencing. Survival rates for BRCA1-familial cases with TP53 mutations were not significantly lower than for familial cases without TP53 mutations (p = 0.25, RR = 1.64, 95% CI [0.71–3.78]). Median age at diagnosis for sporadic (59 years) and familial (49 years) cases differed significantly (p < 0.001) with or without TP53 mutations. Age at diagnosis between the two types of familial carriers were not significantly different, with median age of 47 for 1675delA and 52.5 for 1135insA carriers (p = 0.245). For cases ≥50 years at diagnosis, a trend toward longer survival for sporadic over familial cases was observed (p = 0.08). The opposite trend was observed for cases <50 years at diagnosis. There do not seem to be a protective advantage for familial BRCA1 carriers without TP53 mutations over familial cases with TP53 mutations. However, there seem to be a trend towards initial advantage in survival for familial cases compared to sporadic cases diagnosed before the age of 50 both with and without TP53 mutations. However, this trend diminishes over time and for cases diagnosed ≥50 years the sporadic cases show a trend towards an advantage in survival over familial cases. Although this data set is small, if confirmed, this may be a link in the evidence that the differences in ovarian cancer survival reported, are

Single nucleotide polymorphisms in the TP53 region and susceptibility to invasive epithelial ovarian cancer

DEFF Research Database (Denmark)

Schildkraut, Joellen M; Goode, Ellen L; Clyde, Merlise A

2009-01-01

The p53 protein is critical for multiple cellular functions including cell growth and DNA repair. We assessed whether polymorphisms in the region encoding TP53 were associated with risk of invasive ovarian cancer. The study population includes a total of 5,206 invasive ovarian cancer cases (2,829...

Effects of Baseline Selection on Magnetocardiography: P-Q and T-P Intervals

International Nuclear Information System (INIS)

Lim, Hyun Kyoon; Kwon, Hyuk Chan; Kim, Tae En; Lee, Yong Ho; Kim, Jin Mok; Kim, In Seon; Kim, Ki Woong; Park, Yong Ki

2007-01-01

The baseline selection is the first and important step to analyze magnetocardiography (MCG) parameters. There are no difficulties to select the baseline between P- and Q-wave peak (P-Q interval) of MCG wave recorded from healthy subjects because the P-Q intervals of the healthy subjects do not much vary. However, patients with ischemic heart disease often show an unstable P-Q interval which does not seem to be appropriate for the baseline. In this case, T-P interval is alternatively recommended for the baseline. However, there has been no study on the difference made by the baseline selection. In this study, we studied the effect of the different baseline selection. MCG data were analyzed from twenty healthy subjects and twenty one patients whose baselines were alternatively selected in the T-P interval for their inappropriate P-Q interval. Paired T-test was used to compare two set of data. Fifteen parameters derived from the R-wave peak, the T-wave peak, and the period, T max/3 ∼ T max were compared for the different baseline selection. As a result, most parameters did not show significant differences (p>0.05) except few parameters. Therefore, there will be no significant differences if anyone of two intervals were selected for the MCG baseline. However, for the consistent analysis, P-Q interval is strongly recommended for the baseline correction.

Flash kinetics in liquefied noble gases: Studies of alkane activation and ligand dynamics at rhodium carbonyl centers, and a search for xenon-carbene adducts

Energy Technology Data Exchange (ETDEWEB)

Yeston, Jake Simon [Univ. of California, Berkeley, CA (United States)

2001-01-01

A general introduction is given to place the subsequent chapters in context for the nonspecialist. Results are presented from a low temperature infrared (IR) flash kinetic study of C-H bond activation via photoinduced reaction of Cp*Rh(CO)₂ (1) with linear and cyclic alkanes in liquid krypton and liquid xenon solution. No reaction was observed with methane; for all other hydrocarbons studied, the rate law supports fragmentation of the overall reaction into an alkane binding step followed by an oxidative addition step. For the binding step, larger alkanes within each series (linear and cyclic) interact more strongly than smaller alkanes with the Rh center. The second step, oxidative addition of the C-H bond across Rh, exhibits very little variance in the series of linear alkanes, while in the cyclic series the rate decreases with increasing alkane size. Results are presented from an IR flash kinetic study of the photoinduced chemistry of Tp*Rh(CO)₂ (5; Tp* = hydridotris(3,5-dimethylpyrazolyl)borato) in liquid xenon solution at –50 °C. IR spectra of the solution taken 2 μs after 308 nm photolysis exhibit two transient bands at 1972-1980 cm^-1 and 1992-2000 cm^-1, respectively. These bands were assigned to (η³-Tp*)Rh(CO)•Xe and (η²-Tp*)Rh(CO)•Xe solvates on the basis of companion studies using Bp*Rh(CO)₂ (9; Bp* = dihydridobis(3,5-dimethyl pyrazolyl)borato). Preliminary kinetic data for reaction of 5 with cyclohexane in xenon solution indicate that both transient bands still appear and that their rates of decay correlate with formation of the product Tp*Rh(CO)(C₆H₁₁)(H). The preparation and reactivity of the new complex Bp*Rh(CO)(pyridine) (11) are described. The complex reacts with CH₃I to yield the novel Rh carbene hydride complex HB(Me₂pz)₂Rh(H)(I)(C₅H₅N)(C(O)Me) (12), resulting from formal addition of CH

La administración educativa y su influencia en el rendimiento académico de los alumnos del nivel secundario en Puno

OpenAIRE

Quispe Sairitupa, Míriam Edith

2011-01-01

La tesis titulada "La Administración Educativa y su Influencia en el Rendimiento Académico de los Alumnos del Nivel Secundario en Puno" se desarrolló en la ciudad de Puno en el año lectivo 2009, con ocho instituciones educativas del nivel secundario. El objetivo fue determinar la influencia que ejerce la administración educativa en el rendimiento académico de los estudiantes del nivel secundario de la ciudad de Puno, así como establecer el rendimiento académico de los estudiantes cuya direcci...

Influencia de la televisión en los niños Análisis a través del dibujo infantil

OpenAIRE

Jiménez Yanez, César; Mancinas Chaves, Rosalba

2013-01-01

Este artículo presenta los resultados de un estudio sobre la influencia de la televisión en los niños para el cual se utilizó el dibujo infantil como instrumento de análisis. La televisión, su influencia y el impacto que causa en diversos sectores de la población es un tema bastante estudiado desde distintas disciplinas, en este trabajo el uso del dibujo como herramienta y mecanismo de análisis, es la novedad. El dibujo infantil es un medio de expresión que aporta nuevos elementos para la dis...

TP53 mutations in serous tubal intraepithelial carcinoma and concurrent pelvic high-grade serous carcinoma—evidence supporting the clonal relationship of the two lesions

Science.gov (United States)

Kuhn, Elisabetta; Kurman, Robert J; Vang, Russell; Sehdev, Ann Smith; Han, Guangming; Soslow, Robert; Wang, Tian-Li; Shih, Ie-Ming

2016-01-01

Serous tubal intraepithelial carcinomas (STICs) have been proposed to be the most likely precursor of ovarian, tubal and ‘primary peritoneal’ (pelvic) high-grade serous carcinoma (HGSC). As somatic mutation of TP53 is the most common molecular genetic change of ovarian HGSC, occurring in more than 95% of cases, we undertook a mutational analysis of 29 pelvic HGSCs that had concurrent STICs to demonstrate the clonal relationship of STICs and HGSCs. In addition, we correlated the mutational data with p53 immunostaining to determine the role of p53 immunoreactivity as a surrogate for TP53 mutations in histological diagnosis. Somatic TP53 mutations were detected in all 29 HGSCs analysed and the identical mutations were detected in 27 of 29 pairs of STICs and concurrent HGSCs. Missense mutations were observed in 61% of STICs and frameshift/splicing junction/nonsense mutations in 39%. Interestingly, there were two HGSCs with two distinctly different TP53 mutations each, but only one of the mutations was detected in the concurrent STICs. Missense mutations were associated with intense and diffuse (≥ 60%) p53 nuclear immunoreactivity, while most of the null mutations were associated with complete loss of p53 staining (p STIC and pelvic HGSC and demonstrate the utility of p53 immunostaining as a surrogate for TP53 mutation in the histological diagnosis of STIC. In this regard, it is important to appreciate the significance of different staining patterns. Specifically, strong diffuse staining correlates with a missense mutation, whereas complete absence of staining correlates with null mutations. PMID:21990067

INFLUENCIA DEL TEMPO DE LA MÚSICA EN LAS EMOCIONES

OpenAIRE

Jonathan Azael Caballero-Meneses; Marina Menez

2010-01-01

Se sabe que la música puede influir en dos componentes de las emociones: el arousal y la valencia. En particular, se ha reportado que el tempo o rapidez de la música está directamente relacionado con el arousal, aunque su relación con la valencia o medida de afecto no es aún clara. En este trabajo se analizó la influencia del tempo en ambos componentes mediante la exposición de 24 estudiantes de licenciatura a treinta extractos musicales de distinto tempo. Además se evaluó la posible m...

Human Papillomavirus 16 Infection and TP53 Mutation: Two Distinct Pathogeneses for Oropharyngeal Squamous Cell Carcinoma in an Eastern Chinese Population.

Directory of Open Access Journals (Sweden)

Zhen Wang

Full Text Available To investigate the clinicopathological characteristics, human papillomavirus (HPV infection, p53 expression, and TP53 mutations in oropharyngeal squamous cell carcinoma (OPSCC and determine their utility as prognostic predictors in a primarily eastern Chinese population.The HPV infection status was tested via p16INK4A immunohistochemistry and validated using PCR, reverse blot hybridization and in situ hybridization (ISH in 188 OPSCC samples. p53 expression levels and TP53 gene mutations were assessed through immunohistochemistry and sequencing, respectively. Clinicopathological characteristics and follow-up information were collected. Overall survival was estimated using the Log-rank test.Overall, 22 of the 188 OPSCC samples were associated with HPV infection. HPV16 was identified in all 22 samples, whereas no samples were positive for HPV18. All 22 HPV-associated OPSCC samples were p53 negative and lacked TP53 mutations. HPV16 positivity, female patients, non-smokers, and patients with histological grade I and stage N0 diseases showed better overall survival (p = 0.009, 0.003, 0.048, 0.009, and 0.004, respectively. No significant differences in overall survival between smoking and non-smoking patients were observed in the HPV-associated OPSCC group. Patients without mutations in TP53 exons 5-8 had better prognoses (p = 0.031 among the 43 sequenced specimens. Multivariate analysis indicated that HPV16 infection status (p = 0.011, histological grade (p = 0.017, and N stage (p = 0.019 were independent prognostic factors for patients with OPSCC.Distinct from the situation in Europe and America, for the patients with OPSCC in this study, HPV16 infection was relatively low, although it was still the most important independent prognostic predictor for the disease. In addition to the high smoking and drinking rate in this population, HPV16 infection and TP53 dysfunction appear to be two distinct pathogens for OPSCC patients in the eastern Chinese

Human Papillomavirus 16 Infection and TP53 Mutation: Two Distinct Pathogeneses for Oropharyngeal Squamous Cell Carcinoma in an Eastern Chinese Population.

Science.gov (United States)

Wang, Zhen; Xia, Rong-Hui; Ye, Dong-Xia; Li, Jiang

2016-01-01

To investigate the clinicopathological characteristics, human papillomavirus (HPV) infection, p53 expression, and TP53 mutations in oropharyngeal squamous cell carcinoma (OPSCC) and determine their utility as prognostic predictors in a primarily eastern Chinese population. The HPV infection status was tested via p16INK4A immunohistochemistry and validated using PCR, reverse blot hybridization and in situ hybridization (ISH) in 188 OPSCC samples. p53 expression levels and TP53 gene mutations were assessed through immunohistochemistry and sequencing, respectively. Clinicopathological characteristics and follow-up information were collected. Overall survival was estimated using the Log-rank test. Overall, 22 of the 188 OPSCC samples were associated with HPV infection. HPV16 was identified in all 22 samples, whereas no samples were positive for HPV18. All 22 HPV-associated OPSCC samples were p53 negative and lacked TP53 mutations. HPV16 positivity, female patients, non-smokers, and patients with histological grade I and stage N0 diseases showed better overall survival (p = 0.009, 0.003, 0.048, 0.009, and 0.004, respectively). No significant differences in overall survival between smoking and non-smoking patients were observed in the HPV-associated OPSCC group. Patients without mutations in TP53 exons 5-8 had better prognoses (p = 0.031) among the 43 sequenced specimens. Multivariate analysis indicated that HPV16 infection status (p = 0.011), histological grade (p = 0.017), and N stage (p = 0.019) were independent prognostic factors for patients with OPSCC. Distinct from the situation in Europe and America, for the patients with OPSCC in this study, HPV16 infection was relatively low, although it was still the most important independent prognostic predictor for the disease. In addition to the high smoking and drinking rate in this population, HPV16 infection and TP53 dysfunction appear to be two distinct pathogens for OPSCC patients in the eastern Chinese population.

Meta-analysis of association between the TP53 Arg72Pro polymorphism and risk of endometriosis based on case-control studies.

Science.gov (United States)

Yan, Yulan; Wu, Renzheng; Li, Shaojing; He, Jinlong

2015-06-01

In the light of the relationship between the TP53 Arg72Pro (rs1042522) polymorphism and the risk of endometriosis remains inclusive or controversial. For better understanding of the effect of TP53 Arg72Pro polymorphism on endometriosis risk, we performed a meta-analysis. The relevant studies were identified through a search of PubMed, Web of Science, EMBASE, Ovid, Springer, China National Knowledge Infrastructure (CNKI), cqvip, Wanfang database, and Chinese Biomedical Literature (CBM) databases up to December, 2014. The association between the TP53 Arg72Pro polymorphism and endometriosis risk was pooled by conducted by odds ratios and 95% confidence intervals. A total of fifteen case-control studies with 2683 cases and 3335 controls were eventually identified. There was significant association between Arg72Pro polymorphism and endometriosis risk in all of the five models in overall populations (C vs. G: OR=1.32, 95%CI=1.14-1.53, p=0.00; CC vs. GG: OR=1.80, 95%CI=1.28-2.53, p=0.001; GC vs. GG: OR=1.52, 95%CI=1.22-1.88, p=0.00; CC vs. OR=1.32, 95%CI=1.05-1.66, p=0.016; CC/GC vs. GG: OR=1.59, 95%CI=1.26-2.00, p=0.00). In the sub-group analysis according to ethnicity, the results suggested that TP53 Arg72Pro polymorphism was not associated with endometriosis risk in Caucasians. However, the significant association was found in Asians and Mixed race (MIX) under the five models. The results of this meta-analysis suggest that the TP53 Arg72Pro polymorphism can increase the risk of endometriosis, especially among Asians and MIX populations. Considering the limited sample size and ethnicities included in the meta-analysis, further larger scaled and well-designed studies are needed to confirm our results. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Preclinical efficacy of the MDM2 inhibitor RG7112 in MDM2 amplified and TP53 wild-type glioblastomas

Science.gov (United States)

Verreault, Maite; Schmitt, Charlotte; Goldwirt, Lauriane; Pelton, Kristine; Haidar, Samer; Levasseur, Camille; Guehennec, Jeremy; Knoff, David; Labussiere, Marianne; Marie, Yannick; Ligon, Azra H.; Mokhtari, Karima; Hoang-Xuan, Khe; Sanson, Marc; Alexander, Brian M; Wen, Patrick Y.; Delattre, Jean-Yves; Ligon, Keith L.; Idbaih, Ahmed

2016-01-01

Rationale p53 pathway alterations are key molecular events in glioblastoma (GBM). MDM2 inhibitors increase expression and stability of p53 and are presumed to be most efficacious in patients with TP53 wild-type and MDM2-amplified cancers. However, this biomarker hypothesis has not been tested in patients or patient-derived models for GBM. Methods We performed a preclinical evaluation of RG7112 MDM2 inhibitor, across a panel of 36 patient-derived GBM cell lines (PDCLs), each genetically characterized according to their P53 pathway status. We then performed a pharmacokinetic (PK) profiling of RG7112 distribution in mice and evaluated the therapeutic activity of RG7112 in orthotopic and subcutaneous GBM models. Results MDM2-amplified PDCLs were 44 times more sensitive than TP53 mutated lines that showed complete resistance at therapeutically attainable concentrations (avg. IC50 of 0.52 μM vs 21.9 μM). MDM4 amplified PDCLs were highly sensitive but showed intermediate response (avg. IC50 of 1.2 μM), whereas response was heterogeneous in TP53 wild-type PDCLs with normal MDM2/4 levels (avg. IC50 of 7.7 μM). In MDM2-amplified lines, RG7112 restored p53 activity inducing robust p21 expression and apoptosis. PK profiling of RG7112-treated PDCL intracranial xenografts demonstrated that the compound significantly crosses the blood-brain and the blood-tumor barriers. Most importantly, treatment of MDM2-amplified/TP53 wild-type PDCL-derived model (subcutaneous and orthotopic) reduced tumor growth, was cytotoxic, and significantly increased survival. Conclusion These data strongly support development of MDM2 inhibitors for clinical testing in MDM2-amplified GBM patients. Moreover, significant efficacy in a subset of non-MDM2 amplified models suggests that additional markers of response to MDM2 inhibitors must be identified. PMID:26482041

Microstructure characterization in domestically-made TP310HNbN austenitic stainless steel after creep test

Science.gov (United States)

Guo, Yan; Lin, Lin; Hou, Shufang; Wang, Bohan

Microstructure characterization of domestically-made TP310HNbN austenitic stainless steel after creep test was investigated by means of transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The results revealed that M23C6 carbides precipitated both inside grains and at the grain boundaries and NbCrN particles were located inside grains for creep-rupture samples. It was clear that sigma phase and NbC particles precipitated inside grains for the creep-rupture sample at 670 C. M23C6 carbides with lattice parameter of three times of the austenite matrix grow in a cube to cube orientation relationship with the matrix. The amount of M23C6 carbide particles obviously increased with the testing time prolonged. Deformation hardening induced an enhanced hardness nearby rupture surface for the creep-rupture samples with a short testing time. For the domestically-made TP310HNbN steel, great attention should be paid to the distribution, size and amount of sigma phase and M23C6 during service.

Influencia del modelo elástico en la dinámica de estructuras articuladas

OpenAIRE

Muñoz, M.; López-Hernández, E.

1997-01-01

Se estudia la influencia en la dinámica de una estructura articulada de barras de madera, de la aproximación a las propiedades tensión-deformación del material. Se ha adoptado un método de cálculo por diferencias finitas en el dominio del tiempo, con un modelo de masas concentradas en los nodos.

La influencia de la publicidad infantil de alimentos: antecedentes y estado de la cuestión

Directory of Open Access Journals (Sweden)

Cristina González Díaz

2013-11-01

Full Text Available Actualmente estamos asistiendo a un incesante bombardeo de noticias que nos muestran el tipo de productos que la publicidad de alimentos, dirigida específicamente al target infantil, publicita: productos hipercalóricos, con altos niveles en azúcares y sal, etc; en definitiva, productos poco recomendables en la dieta del niño. Al mismo tiempo, no cesan de publicarse investigaciones académicas sobre el poder persuasivo y la capacidad de influencia que este tipo concreto de publicidad posee sobre el niño. Bajo este contexto, este artículo trata de abordar los estudios académicos más significativos que han investigado la posible relación entre la publicidad de alimentos dirigida a niños y la problemática de la obesidad, o más a grandes rasgos, la influencia que la publicidad específica de este sector puede tener en la ingesta de alimentos poco saludables

Uso e influencia de la televisión en la infancia

OpenAIRE

Arroyo Jiménez, Isabel

2012-01-01

Esta investigación está contextualizada en torno al uso e influencia que ejerce la televisión en la infancia. En concreto hemos escogido un grupo de niños del tercer ciclo de Educación Primaria (10-12 años). Primeramente, tratamos de comprobar el uso que hacen de la televisión los alumnos que hemos tomado como muestra así como el tipo de programas que ven. Posteriormente, analizaremos una pequeña muestra de esos programas más seguidos por nuestros alumnos encuestados. Para ello, partire...

La motivación laboral y su influencia en la empresa

OpenAIRE

Jiménez Cacho, Raquel

2017-01-01

Este proyecto se centra en un tema de gran interés como es la motivación laboral y su influencia en la empresa. Como sabemos este tema no es nuevo y se ha analizado y estudiado desde hace mucho tiempo, debido a la incidencia que tiene en el mundo laboral, económico y social. La motivación laboral lleva una trayectoria positiva en cuanto a su estudio e importancia, gracias a su estrecha relación con los problemas, soluciones, costes, beneficios y ventajas competitivas de las empresas. Para ...

Field test results for steam oxidation of TP347H FG - growth of inner oxide

DEFF Research Database (Denmark)

Montgomery, Melanie; Jianmin, Jia; Larsen, OH

2005-01-01

A series of field tests have been conducted with TP347H FG in test superheater loops in coal-fired and biomass fired boilers of steam pressure 256 and 91 bar respectively. The exposure times are from 3,500 to 30,000 hours and the temperature range is from 450-630¢XC. The morphology, composition...

Inhibition of A2A Adenosine Receptor Signaling in Cancer Cells Proliferation by the Novel Antagonist TP455

Directory of Open Access Journals (Sweden)

Stefania Gessi

2017-12-01

Full Text Available Several evidences indicate that the ubiquitous nucleoside adenosine, acting through A1, A2A, A2B, and A3 receptor (AR subtypes, plays crucial roles in tumor development. Adenosine has contrasting effects on cell proliferation depending on the engagement of different receptor subtypes in various tumors. The involvement of A2AARs in human A375 melanoma, as well as in human A549 lung and rat MRMT1 breast carcinoma proliferation has been evaluated in view of the availability of a novel A2AAR antagonist, with high affinity and selectivity, named as 2-(2-furanyl-N5-(2-methoxybenzyl[1,3]thiazolo[5,4-d]pyrimidine-5,7-diammine (TP455. Specifically, the signaling pathways triggered in the cancer cells of different origin and the antagonist effect of TP455 were investigated. The A2AAR protein expression was evaluated through receptor binding assays. Furthermore, the effect of A2AAR activation on cell proliferation at 24, 48 and 72 hours was studied. The selective A2AAR agonist 2-p-(2-carboxyethylphenethylamino-5′-N-ethylcarboxamidoadenosine hydrochloride (CGS21680, concentration-dependently induced cell proliferation in A375, A549, and MRMT1 cancer cells and the effect was potently antagonized by the A2AAR antagonist TP455, as well as by the reference A2AAR blocker 4-(2-[7-amino-2-(2-furyl[1,2,4]triazolo[2,3-a][1,3,5]triazin-5-ylamino]ethylphenol (ZM241385. As for the signaling pathway recruited in this response we demonstrated that, by using the specific inhibitors of signal transduction pathways, the effect of A2AAR stimulation was induced through phospholipase C (PLC and protein kinase C-delta (PKC-δ. In addition, we evaluated, through the AlphaScreen SureFire phospho(p protein assay, the kinases enrolled by A2AAR to stimulate cell proliferation and we found the involvement of protein kinase B (AKT, extracellular regulated kinases (ERK1/2, and c-Jun N-terminal kinases (JNKs. Indeed, we demonstrated that the CGS21680 stimulatory effect on kinases was

TP53 mutations in serous tubal intraepithelial carcinoma and concurrent pelvic high-grade serous carcinoma--evidence supporting the clonal relationship of the two lesions.

Science.gov (United States)

Kuhn, Elisabetta; Kurman, Robert J; Vang, Russell; Sehdev, Ann Smith; Han, Guangming; Soslow, Robert; Wang, Tian-Li; Shih, Ie-Ming

2012-02-01

Serous tubal intraepithelial carcinomas (STICs) have been proposed to be the most likely precursor of ovarian, tubal and 'primary peritoneal' (pelvic) high-grade serous carcinoma (HGSC). As somatic mutation of TP53 is the most common molecular genetic change of ovarian HGSC, occurring in more than 95% of cases, we undertook a mutational analysis of 29 pelvic HGSCs that had concurrent STICs to demonstrate the clonal relationship of STICs and HGSCs. In addition, we correlated the mutational data with p53 immunostaining to determine the role of p53 immunoreactivity as a surrogate for TP53 mutations in histological diagnosis. Somatic TP53 mutations were detected in all 29 HGSCs analysed and the identical mutations were detected in 27 of 29 pairs of STICs and concurrent HGSCs. Missense mutations were observed in 61% of STICs and frameshift/splicing junction/nonsense mutations in 39%. Interestingly, there were two HGSCs with two distinctly different TP53 mutations each, but only one of the mutations was detected in the concurrent STICs. Missense mutations were associated with intense and diffuse (≥ 60%) p53 nuclear immunoreactivity, while most of the null mutations were associated with complete loss of p53 staining (p STIC and pelvic HGSC and demonstrate the utility of p53 immunostaining as a surrogate for TP53 mutation in the histological diagnosis of STIC. In this regard, it is important to appreciate the significance of different staining patterns. Specifically, strong diffuse staining correlates with a missense mutation, whereas complete absence of staining correlates with null mutations. Copyright © 2012 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

La centralidad en las comunicaciones y la influencia percibida en los pequeños grupos

Directory of Open Access Journals (Sweden)

Nancy Noemi Terroni

Full Text Available Este trabajo reporta los resultados de medidas del análisis de redes en la comunicación de pequeños grupos que resuelven una tarea de recuperación de memoria y su asociación con la influencia percibida. El reactivo empleado es una historia y los 65 participantes, alumnos de la Universidad Nacional de Mar del Plata, debieron reconstruir la misma primero en forma individual, luego grupal colaborativa y nuevamente en forma individual. Se registraron las interacciones grupales con comunicación cara a cara y mediada por computadora; se analizaron las medidas de prominencia, la centralidad y el prestigio de la comunicación y el tipo de alocución, orientado a la gestión grupal o hacia la tarea. Se observaron asociaciones significativas entre las medidas reticulares y la influencia percibida para ambos medios de comunicación y se hallaron diferencias en la comunicación de gestión grupal. Se discuten estos resultados con relación a las restricciones que imponen los canales de comunicación mediados.

Evolución de la inmunología e influencia en los trasplantes de órganos

OpenAIRE

Carretero Vegas, Luz María

2012-01-01

Se profundiza en el conocimiento sobre los mecanismos del sistema inmunológico y la evolución histórica de los mismos para conocer la influencia de la inmunología en los trasplantes y las medidas preventivas más adecuadas para evitar las complicaciones

Field Investigation of Steamside Oxidation for TP347H

DEFF Research Database (Denmark)

Montgomery, Melanie; Larsen, Ole Hede; Jensen, Søren Aakjær

2004-01-01

The steamside oxide formed on two TP347H superheater tubes was compared. The two specimens investigated were exposed in situ in power plants in Denmark, one specimen was coarse-grained and the other was fine-grained. Parts of both the coarse-grained and the fine-grained specimens were turned...... (machined on the inner side) to give a constant metal thickness so more precise wall thickness measurements could be undertaken. Machined and non-machined areas were investigated using light optical microscopy, and scanning electron microscopy with EDS analysis. The oxide on the fine-grained specimen...... was also investigated with grazing incidence X-ray diffraction. Results from coarse-grained and fine-grained specimens (machined and non-machined) show that grain size influenced oxide thickness and morphology. The oxides from non-machined specimens has an outer iron rich oxide and an inner iron chromium...

[Evaluation of the Recombinant Protein Tp0965 of Treponema Pallidum as Perspective Antigen for the Improved Serological Diagnosis of Syphilis].

Science.gov (United States)

Runina, A V; Starovoitova, A S; Deryabin, D G; Kubanov, A A

2016-01-01

BACKGRAUND. Treponemal tests based on the detection of antibodies against the Treponema pallidum antigens are the most specific methods for serological diagnosis of syphilis. Due to the inability to cultivate this bacterium in vitro, the most promising sources of antigens for diagnostics are recombinant proteins of T. pallidum. Evaluation of the analytical value of certain T. pallidum proteins is the approach to improve sensitivity, specificity, and reproducibility of syphilis serological tests, including possibilities of differential diagnosis of various forms of the disease. The aim of the research was to evaluate the analytical values (sensitivity and specificity) of recombinant protein Tp0965 of T. pallidum as a candidate antigen for serological diagnosis of syphilis. tp0965 gene was cloned into the expression vector pET28a and the construct was used for the transformation of E. coli BL-21 (DE3) cells and further expression and purification of the recombinant protein. The collected protein was used as T. pallidum antigen for serum analysis (ELISA) of groups of patients with various forms of syphilis (n=84) and the group of healthy donors (n = 25). High frequency of positive ELISA results was shown with serum of patients with syphilis, compared to the group of healthy donors. The sensitivity of serological reactions using recombinant protein Tp0965 was 98.8%, specificity--87.5%. The highest sensitivity (100%) was detected in the groups of patients with primary, secondary and early latent syphilis while in the group of patients with late latent syphilis it decreased to 95.2%. We concluded that due to its specificity T. pallidum recombinant protein Tp0965 can be used as a novel perspective antigen for development of syphilis serological diagnostic assays (for primary and early latent forms).

Material problems related to large scale firing of biomass. Steam oxidation of TP 347H FG and X20

Energy Technology Data Exchange (ETDEWEB)

Noergaard Hansson, A.

2009-07-01

TP 347 H and X20 is often used as construction material in biomass-fired boilers. The corrosion rate of the alloys is affected by the metal temperature. In this project, the oxidation behaviour of TP 347H FG and X20 is studied by field-testing, laboratory exposures, and thermodynamic/kinetic modelling. The long term oxidation behaviour of TP 347H FG at ultra supercritical steam conditions was assessed by exposing the steel in 4 test superheater loops in a coal-fired power plant. The steel was exposed for 7720, 22985, 29588, and 57554 h at metal temperatures between 499 and 650 deg. C. In the laboratory furnace, the oxidation behaviour of TP 347H FG, TP 347H CG, and X20 was studied in water vapour containing environments (8 or 46%) in the temperature range 500-700 deg. C. Air, Ar and Ar+7% H{sub 2} were used as carrier gas. The microstructure of the oxide layer and the subjacent alloy was investigated with X-ray diffraction (XRD), reflective light microscopy (RLM), scanning electron microscopy (SEM) equipped with energy dispersive spectroscopy (EDS), and transmission electron microscopy (TEM) equipped with electron diffraction (ED) and EDS. Double-layered oxides developed during steam oxidation of TP 347H FG both during field-testing and during laboratory exposures. TEM investigation suggested that the interior of the alloy grain was oxidised internally, forming particles of metallic Ni/Fe and Fe-Cr spinel. A FeCr{sub 2}O{sub 4} layer developed along the former alloy grain boundaries. Cr{sub 2}O{sub 3} developed in between this layer and the alloy during field-testing, and its formation was promoted by higher temperature. The morphology of the inner layer for the samples oxidised below and above approx. 585 deg. C looked very different in SEM (field-testing). It is suggested that more Cr is incorporated into the oxide layer at higher temperature, gradually transforming the morphology of the inner oxide layer. The alloy beneath the oxide layer was depleted in Cr

THE CONTRIBUTION OF TP-AGB AND RHeB STARS TO THE NEAR-IR LUMINOSITY OF LOCAL GALAXIES: IMPLICATIONS FOR STELLAR MASS MEASUREMENTS OF HIGH-REDSHIFT GALAXIES

International Nuclear Information System (INIS)

Melbourne, J.; Williams, Benjamin F.; Dalcanton, Julianne J.; Rosenfield, Philip; Weisz, D.

2012-01-01

Using high spatial resolution Hubble Space Telescope WFC3 and Advanced Camera for Surveys imaging of resolved stellar populations, we constrain the contribution of thermally pulsing asymptotic giant branch (TP-AGB) stars and red helium burning (RHeB) stars to the 1.6 μm near-infrared (NIR) luminosities of 23 nearby galaxies, including dwarfs and spirals. The TP-AGB phase contributes as much as 17% of the integrated F160W flux, even when the red giant branch is well populated. The RHeB population contribution can match or even exceed the TP-AGB contribution, providing as much as 21% (18% after a statistical correction for foreground) of the integrated F160W light. We estimate that these two short-lived phases may account for up to 70% of the rest-frame NIR flux at higher redshift. The NIR mass-to-light (M/L) ratio should therefore be expected to vary significantly due to fluctuations in the star formation rate (SFR) over timescales from 25 Myr to several Gyr, an effect that may be responsible for some of the lingering scatter in NIR galaxy scaling relations such as the Tully-Fisher and metallicity-luminosity relations. We compare our observational results to predictions based on optically derived star formation histories and stellar population synthesis (SPS) models, including models based on the 2008 Padova isochrones (used in popular SPS programs) and the updated 2010 Padova isochrones, which shorten the lifetimes of low-mass (old) low-metallicity TP-AGB populations. The updated (2010) SPS models generally reproduce the expected numbers of TP-AGB stars in the sample; indeed, for 65% of the galaxies, the discrepancy between modeled and observed numbers is smaller than the measurement uncertainties. The weighted mean model/data number ratio for TP-AGB stars is 1.5 (1.4 with outliers removed) with a standard deviation of 0.5. The same SPS models, however, give a larger discrepancy in the F160W flux contribution from the TP-AGB stars, overpredicting the flux by a

Haplotype analysis of TP53 polymorphisms, Arg72Pro and Ins16, in BRCA1 and BRCA2 mutation carriers of French Canadian descent

International Nuclear Information System (INIS)

Cavallone, Luca; Arcand, Suzanna L; Maugard, Christine; Ghadirian, Parviz; Mes-Masson, Anne-Marie; Provencher, Diane; Tonin, Patricia N

2008-01-01

The TP53 polymorphisms Arg72Pro (Ex4+199 G>C) and Ins16 (IVS3+24 ins16) have been proposed to modify risk of breast cancer associated with germline BRCA1 and BRCA2 mutations. Allele frequencies of these polymorphisms were investigated to determine if they modify risk in BRCA mutation carriers in breast cancer cases drawn from French Canadian cancer families, a population shown to exhibit strong founder effects. The frequencies of the TP53 alleles, genotypes and haplotypes of 157 index breast cancer cases comprised of 42 BRCA1 mutation carriers, 57 BRCA2 mutation carriers, and 58 BRCA mutation-negative cases, where each case was drawn from independently ascertained families were compared. The effect of TP53 variants on the age of diagnosis was also investigated for these groups. The TP53 polymorphisms were also investigated in 112 women of French Canadian descent with no personal history of cancer. The BRCA mutation-positive groups had the highest frequency of homozygous carriers of the 72Pro allele compared with mutation-negative group. The TP53 polymorphisms exhibited linkage disequilibrium (p < 0.001), where the 72Arg and Ins16minus alleles occurred in strong disequilibrium. The highest frequency of carriers of Ins16minus-72Arg haplotype occurred in the BRCA mutation-negative groups. The BRCA1 mutation carriers homozygous for the 72Pro allele had the youngest ages of diagnosis of breast cancer. However none of these observations were statistically significant. In contrast, the BRCA2 mutation carriers homozygous for the 72Pro allele had a significantly older age of diagnosis of breast cancer (p = 0.018). Moreover, in this group, the mean age of diagnosis of breast cancer in carriers of the Ins16minus-72Arg haplotype was significantly younger than that of the individuals who did not this carry this haplotype (p = 0.009). We observed no significant association of breast cancer risk with TP53 genetic variants based on BRCA1/2 mutation carrier status. Although the

Influencia léxica del muisca en el español actual clasificada en campos semántico

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DIANA ANDREA GIRALDO GALLEGO

2014-01-01

Full Text Available La presencia léxica del muisca en el dialecto cundiboyacense es una muestra de que esta influencia aún sigue vigente. El objetivo de este artículo es presentar los préstamos y posibles préstamos del muisca en el español actual clasificados en campos semánticos. Los datos se recolectaron en los municipios de Machetá y Tibirita en el departamento de Cundinamarca; Guateque, Sutatenza, Guayatá, La Capilla, Garagoa, Chinavita y Pachavita en el departamento de Boyacá. Los resultados se organizaron en siete campos semánticos: campo y cultivo; minería; espacio; animales; familia y ciclo de vida; cuerpo humano; recipientes, alimentación y vivienda. Se concluyó que los campos que presentan una mayor influencia son los que corresponden a cultivo y animales.

Identification of Quaternary Structure and Functional Domains of the CI Repressor from Bacteriophage TP901-1

DEFF Research Database (Denmark)

Pedersen, Margit; Lo Leggio, Leila; Grossmann, J. Günter

2008-01-01

is involved in the interaction with host proteins. By using small-angle X-ray scattering, we show for the first time the overall solution structure of a full-length wild-type bacteriophage repressor at low resolution revealing that the TP901-1 repressor forms a flat oligomer, most probably a trimer of dimers....

Temporal transcription of the lactococcal temperate phage TP901-1 and DNA sequence of the early promoter region

DEFF Research Database (Denmark)

Madsen, Hans Peter Lynge; Hammer, Karin

1998-01-01

to a phage repressor, a single-stranded DNA-binding protein, a topoisomerase, a Cro-like protein and two other phage proteins of unknown function were detected. The gene arrangement in the early transcribed region of TP901-1 thus consists of two transcriptional units: one from PR containing four genes......, of which at least two (the integrase gene and putative repressor) are needed for lysogeny, and the divergent and longer transcriptional unit from PL, presumably encoding functions required for the lytic life cycle. ORFs with homology to proteins involved in DNA replication were identified on the latter......Transcriptional analysis by Northern blotting identified clusters of early, middle and late transcribed regions of the temperate lactococcal bacteriophage TP901-1 during one-step growth experiments. The latent period was found to be 65 min and the burst size 40 +/- 10. The eight early transcripts...

Influencia del medio familiar en niños con conductas agresivas

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Nilo Valentín Noroño Morales

2002-06-01

Full Text Available Se realizó un estudio descriptivo, sobre la influencia del medio familiar en niños de 9 a 11 años, con conductas agresivas, evaluados y diagnosticados en el Departamento de Psicología del Policlínico " Dr. Tomás Romay"; la muestra estuvo conformada además, por los padres de estos niños. A todos ellos se les aplicó una serie de técnicas con el objetivo principal de describir las características del medio familiar y su influencia en las conductas agresivas de los infantes. Se concluyó en que como características del medio familiar predominaron las familias disfuncionales incompletas, con manifestaciones de agresividad, alcoholismo, mala integración social y familiar, rechazo hacia los hijos e irresponsabilidad en su cuidado y atención.A descriptive study of the influence of the family environment on children aged 9-11 years old, evaluated and diagnosed at the Psychology Department of "Dr Tomás Romay" polyclinics, was carried out. The children´s parents were also part of the sample. All of them were applied a series of techniques to find out the characteristics of the family environment and its influence on children´s aggressive behaviors. It was concluded that the predominant characteristics were uncompleted dysfunctional families with manifestations of aggressiveness, alcoholism, poor social and family integration, rejection to children and lack of responsibility in relation to their care and attention.

Estimates of long-term water total phosphorus (TP) concentrations in three large shallow lakes in the Yangtze River basin, China.

Science.gov (United States)

Wu, Pan; Qin, Boqiang; Yu, Ge

2016-03-01

The shallow lakes in the eastern China developed on alluvial plains with high-nutrient sediments, and most overflow into the Yangtze River with short hydraulic residence times, whereas they become eutrophic over long time periods. Assuming strong responses to hydrogeological changes in the basin, we attempted to determine the dynamic eutrophication history of these lakes. Although evaluation models for internal total phosphorus (TP) loading are widely used for deep lakes in Europe and North America, the accuracy of these models for shallow lakes that have smaller water volumes controlled by the geometrical morphology and greater basin area of alluvial plains is unknown. To describe the magnitude of changes in velocity of trophic state for the studied shallow lakes, we first evaluated the P retention model in relation to the major forces driving lake morphology, basin climate, and external discharge and then used the model to estimate changes in TP in three large shallow lakes (Taihu, Chao, and Poyang) over 60 years (1950-2009 AD). The observed levels of TP were verified against the relative error of the three lakes (Yangtze River basin. This work will contribute to the development of an internal P loading model for further evaluating trophic states.

TP53 and ATM mRNA expression in skin and skeletal muscle after low-level laser exposure.

Science.gov (United States)

Guedes de Almeida, Luciana; Sergio, Luiz Philippe da Silva; de Paoli, Flavia; Mencalha, Andre Luiz; da Fonseca, Adenilson de Souza

2017-08-01

Low-level lasers are widespread in regenerative medicine, but the molecular mechanisms involved in their biological effects are not fully understood, particularly those on DNA stability. Therefore, this study aimed to investigate mRNA expression of genes related to DNA genomic stability in skin and skeletal muscle tissue from Wistar rats exposed to low-level red and infrared lasers. For this, TP53 (Tumor Protein 53) and ATM (Ataxia Telangiectasia Mutated gene) mRNA expressions were evaluated by real-time quantitative PCR (RT-qPCR) technique 24 hours after low-level red and infrared laser exposure. Our data showed that relative TP53 mRNA expression was not significantly altered in both tissues exposed to lasers. For ATM, relative mRNA expression in skin tissue was not significantly altered, but in muscle tissue, laser exposure increased relative ATM mRNA expression. Low-level red and infrared laser radiations alter ATM mRNA expression related to DNA stability in skeletal muscle tissue.

Unambiguous detection of multiple TP53 gene mutations in AAN-associated urothelial cancer in Belgium using laser capture microdissection.

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Selda Aydin

Full Text Available In the Balkan and Taiwan, the relationship between exposure to aristolochic acid and risk of urothelial neoplasms was inferred from the A>T genetic hallmark in TP53 gene from malignant cells. This study aimed to characterize the TP53 mutational spectrum in urothelial cancers consecutive to Aristolochic Acid Nephropathy in Belgium. Serial frozen tumor sections from female patients (n=5 exposed to aristolochic acid during weight-loss regimen were alternatively used either for p53 immunostaining or laser microdissection. Tissue areas with at least 60% p53-positive nuclei were selected for microdissecting sections according to p53-positive matching areas. All areas appeared to be carcinoma in situ. After DNA extraction, mutations in the TP53 hot spot region (exons 5-8 were identified using nested-PCR and sequencing. False-negative controls consisted in microdissecting fresh-frozen tumor tissues both from a patient with a Li-Fraumeni syndrome who carried a p53 constitutional mutation, and from KRas mutated adenocarcinomas. To rule out false-positive results potentially generated by microdissection and nested-PCR, a phenacetin-associated urothelial carcinoma and normal fresh ureteral tissues (n=4 were processed with high laser power. No unexpected results being identified, molecular analysis was pursued on malignant tissues, showing at least one mutation in all (six different mutations in two patients, with 13/16 exonic (nonsense, 2; missense, 11 and 3/16 intronic (one splice site mutations. They were distributed as transitions (n=7 or transversions (n=9, with an equal prevalence of A>T and G>T (3/16 each. While current results are in line with A>T prevalence previously reported in Balkan and Taiwan studies, they also demonstrate that multiple mutations in the TP53 hot spot region and a high frequency of G>T transversion appear as a complementary signature reflecting the toxicity of a cumulative dose of aristolochic acid ingested over a short period

The genetic switch regulating activity of early promoters of the temperate lactococcal bacteriophage TP901-1

DEFF Research Database (Denmark)

Madsen, P.L.; Johansen, Annette Helle; Hammer, Karin

1999-01-01

A functional analysis of open reading frame 4 (ORF4) and ORF5 from the temperate lactococcal phage TP901-1 was performed by mutant and deletion analysis combined with transcriptional studies of the early phage promoters p(R) and p(L). ORF4 (180 amino acids) was identified as a phage repressor...... necessary for repression of both promoters. Furthermore, the presence of ORF4 confers immunity of the host strain to TP901-1. ORF5 (72 amino acids) was found to be able to inhibit repression of the lytic promoter p(L) by ORF4. Upon transformation with a plasmid containing both ORF4 and ORF5...... and their cognate promoters, clonal variation is observed: in each transformant, either p(L) is open and p(R) is closed or vice versa. The repression is still dependent on ORF4, and the presence of ORF5 is needed for the clonal variation. Induction of a repressed p(L) fusion containing orf4 and orf5 was obtained...

Influencia de la personalidad en la clínica del trastorno de pánico con o sin agorafobia : un estudio exploratorio

OpenAIRE

Osma López, Jorge Javier

2009-01-01

El interés científico por determinar los factores de vulnerabilidad relacionados con la psicopatología ha cobrado relevancia en los últimos años. En este sentido, el estudio de las características de personalidad tanto normal como patológica y su influencia en distintos trastornos mentales es un campo del máximo interés para la psicología clínica y la Psicopatología. El trabajo de tesis doctoral que lleva por título "Influencia de la personalidad en la clínica del trastorno de pánico con o si...

Crystal growth, structural, optical, thermal, mechanical, laser damage threshold and electrical properties of triphenylphosphine oxide 4-nitrophenol (TP4N) single crystals for nonlinear optical applications

Science.gov (United States)

Karuppasamy, P.; Senthil Pandian, Muthu; Ramasamy, P.; Verma, Sunil

2018-05-01

The optically good quality single crystals of triphenylphosphine oxide 4-nitrophenol (TP4N) with maximum dimension of 15 × 10 × 5 mm3 were grown by slow evaporation solution technique (SEST) at room temperature. The cell dimensions of the grown TP4N crystal were confirmed by single crystal X-ray diffraction (SXRD) and the crystalline purity was confirmed and planes were indexed by powder X-ray diffraction (PXRD) analysis. Functional groups of TP4N crystal were confirmed by Fourier transform infrared (FTIR) spectral analysis. The optical transmittance of the grown crystal was determined by the UV-Vis NIR spectral analysis and it has good optical transparency in the entire visible region. The band tail (Urbach) energy of the grown crystal was analyzed and it appears to be minimum, which indicates that the TP4N has good crystallinity. The position of valence band (Ev) and conduction band (Ec) of the TP4N have been determined from the electron affinity energy (EA) and the ionization energy (EI) of its elements and using the optical band gap. The thermal behaviour of the grown crystal was investigated by thermogravimetric and differential thermal analysis (TG-DTA). Vickers microhardness analysis was carried out to identify the mechanical stability of the grown crystal and their indentation size effect (ISE) was explained by the Meyer's law (ML), Hays-Kendall's (HK) approach, proportional specimen resistance (PSR) model, modified PSR model (MPSR), elastic/plastic deformation (EPD) model and indentation induced cracking (IIC) model. Chemical etching study was carried out to find the etch pit density (EPD) of the grown crystal. Laser damage threshold (LDT) value was measured by using Nd:YAG laser (1064 nm). The dielectric permittivity (ε՛) and dielectric loss (tan δ) as a function of frequency was measured. The electronic polarizability (α) of the TP4N crystal was calculated. It is well matched to the value which was calculated from Clausius-Mossotti relation

TP53 codon 72 polymorphism and cervical cancer : a pooled analysis of individual data from 49 studies

NARCIS (Netherlands)

Klug, Stefanie J.; Ressing, Meike; Koenig, Jochem; Abba, Martin C.; Agorastos, Theodoros; Brenna, Sylvia M. F.; Ciotti, Marco; Das, B. R.; Del Mistro, Annarosa; Dybikowska, Aleksandra; Giuliano, Anna R.; Gudleviciene, Zivile; Gyllensten, Ulf; Haws, Andrea L. F.; Helland, Aslaug; Herrington, C. Simon; Hildesheim, Alan; Humbey, Olivier; Jee, Sun H.; Kim, Jae Weon; Madeleine, Margaret M.; Menczer, Joseph; Ngan, Hextan Y. S.; Nishikawa, Akira; Niwa, Yoshimitsu; Pegoraro, Rosemary; Pillai, M. R.; Ranzani, Gulielmina; Rezza, Giovanni; Rosenthal, Adam N.; Roychoudhury, Susanta; Saranath, Dhananjaya; Schmitt, Virginia M.; Sengupta, Sharmila; Settheetham-Ishida, Wannapa; Shirasawa, Hiroshi; Snijders, Peter J. F.; Stoler, Mark H.; Suarez-Rincon, Angel E.; Szarka, Krisztina; Tachezy, Ruth; Ueda, Masatsugu; van der Zee, Ate G. J.; Doeberitz, Magnus von Knebel; Wu, Ming-Tsang; Yamashita, Tsuyoshi; Zehbe, Ingeborg; Blettner, Maria

Background Cervical cancer is caused primarily by human papillomaviruses (HPV). The polymorphism rs1042522 at codon 72 of the TP53 tumour-suppressor gene has been investigated as a genetic cofactor. More than 80 studies were done between 1998 and 2006, after it was initially reported that women who

Gene-gene interaction between MSX1 and TP63 in Asian case-parent trios with nonsyndromic cleft lip with or without cleft palate.

Science.gov (United States)

Liu, Dongjing; Schwender, Holger; Wang, Mengying; Wang, Hong; Wang, Ping; Zhu, Hongping; Zhou, Zhibo; Li, Jing; Wu, Tao; Beaty, Terri H

2018-03-01

Small ubiquitin-like modification, also known as sumoylation, is a crucial post-translational regulatory mechanisms involved in development of the lip and palate. Recent studies reported two sumoylation target genes, MSX1 and TP63, to have achieved genome-wide level significance in tests of association with nonsyndromic clefts. Here, we performed a candidate gene analysis considering gene-gene and gene-environment interaction for SUMO1, MSX1, and TP63 to further explore the etiology of nonsyndromic cleft lip with or without cleft palate (NSCL/P). A total of 130 single-nucleotide polymorphisms (SNPs) in or near SUMO1, MSX1, and TP63 was analyzed among 1,038 Asian NSCL/P trios ascertained through an international consortium. Conditional logistic regression models were used to explore gene-gene (G × G) and gene-environment (G × E) interaction involving maternal environmental tobacco smoke and multivitamin supplementation. Bonferroni correction was used for G × E analysis and permutation tests were used for G × G analysis. While transmission disequilibrium tests and gene-environment interaction analysis showed no significant results, we did find signals of gene-gene interaction between SNPs near MSX1 and TP63. Three pairwise interactions yielded significant p values in permutation tests (rs884690 and rs9290890 with p = 9.34 × 10 -5 and empirical p = 1.00 × 10 -4 , rs1022136 and rs4687098 with p = 2.41 × 10 -4 and empirical p = 2.95 × 10 -4 , rs6819546 and rs9681004 with p = 5.15 × 10 -4 and empirical p = 3.02 × 10 -4 ). Gene-gene interaction between MSX1 and TP63 may influence the risk of NSCL/P in Asian populations. Our study provided additional understanding of the genetic etiology of NSCL/P and underlined the importance of considering gene-gene interaction in the etiology of this common craniofacial malformation. © 2018 Wiley Periodicals, Inc.

Influencia de los factores que definen el modelo estético corporal en el bienestar de las mujeres jóvenes afectadas o no afectadas por anorexia y bulimia

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Victoria Carrillo Durán

2013-06-01

Full Text Available OBJETIVO DEL ESTUDIO: El modelo estético corporal actual viene determinado por diferentes factores personales y socioculturales, siendo necesario profundizar su influencia e identificar las dimensiones en que se agrupan. METODOLOGÍA: Para eso, se evaluó la actitud de una muestra de 95 mujeres (saludables y enfermas de anorexia y bulimia frente a la repercusión de su bienestar en determinados factores relacionados con el modelo estético corporal. En segundo lugar, se realizó un análisis factorial de Componentes Principales para determinar las dimensiones en que se agrupan. RESULTADOS Y CONCLUSIONES: Concluímos, que son tres las dimensiones de influencia extraídas: primera: "la dimensión social y de autoestima", segunda: "la dimensión sociocultural relacionada con los medios de comunicación" y tercera: "la dimensión de influencia del compañero y la aceptación". A pesar de ser mayor la influencia de los factores personales, familia, amigos y compañero, la "dimensión sociocultural mediática" es la que determina una diferencia mayor entre ambos grupos.

Espacios y ambientes comerciales. La influencia del contexto en la eficacia comunicativa

OpenAIRE

Martínez Sánchez, Mª Eugenia; Universitat Autònoma de Barcelona. Departament de Comunicació Audiovisual i Publicitat

2015-01-01

El ambiente que rodea a un consumidor cuando se encuentra en un establecimiento comercial puede provocar efectos sobre él que deriven en una conducta y comportamiento favorable o desfavorable ante los procesos comunicativos que se producen en él. La investigación surgió de la necesidad de averiguar la capacidad de influencia que poseen los componentes del contexto espacial (color de la pared, iluminación, música, etc.) que rodean al sujeto; es decir, conocer cómo intervienen éstos en el ámbit...

Antinociceptive effect of novel pyrazolines in mice

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Tabarelli Z.

2004-01-01

Full Text Available The antinociceptive effect of six novel synthetic pyrazolines (3-ethoxymethyl-5-ethoxycarbonyl-1H-pyrazole (Pz 1 and its corresponding 1-substituted methyl (Pz 2 and phenyl (Pz 3 analogues, and 3-(1-ethoxyethyl-5-ethoxycarbonyl-1H-pyrazole (Pz 4 and its corresponding 1-substituted methyl (Pz 5 and phenyl (Pz 6 analogues was evaluated by the tail immersion test in adult male albino mice. The animals (N = 11-12 in each group received vehicle (5% Tween 80, 10 ml/kg, sc or 1.5 mmol/kg of each of the pyrazolines (Pz 1-Pz 6, sc. Fifteen, thirty and sixty minutes after drug administration, the mice were subjected to the tail immersion test. Thirty minutes after drug administration Pz 2 and Pz 3 increased tail withdrawal latency (vehicle = 3.4 ± 0.2; Pz 2 = 5.2 ± 0.4; Pz 3 = 5.9 ± 0.4 s; mean ± SEM, whereas the other pyrazolines did not present antinociceptive activity. Dose-effect curves (0.15 to 1.5 mmol/kg were constructed for the bioactive pyrazolines. Pz 2 (1.5 mmol/kg, sc impaired motor coordination in the rotarod and increased immobility in the open-field test. Pz 3 did not alter rotarod performance and spontaneous locomotion, but increased immobility in the open field at the dose of 1.5 mmol/kg. The involvement of opioid mechanisms in the pyrazoline-induced antinociception was investigated by pretreating the animals with naloxone (2.75 µmol/kg, sc. Naloxone prevented Pz 3- but not Pz 2-induced antinociception. Moreover, naloxone pretreatment did not alter Pz 3-induced immobility. We conclude that Pz 3-induced antinociception involves opioid mechanisms but this is not the case for Pz 2.

La influencia social de la televisión: fuentes y métodos de estudio

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MANUEL MARTÍN SERRANO

1982-01-01

Full Text Available Se propone una teoría de la influencia de la televisión, basada en valores de objetividad social. Se analizan como defectuosos los estudios previos sobre el papel de la televisión como mediadora de las relaciones entre el productor, el consumidor y el votante, llevados a cabo por empresas de mercadotecnia porque no promueven un mayor uso social de la televisión. La publicidad se basa en la falsificación y ocultamiento de la realidad. La publicidad política compromete a la democracia al apelar a las emociones más que a la discusión racional. El papel de la televisión en los procesos de socialización esta en función de los modelos significativos de ambos, la televisión y su audiencia: donde estos sistemas de valores y referencia coinciden, la influencia de la televisión será fuerte. Se sugiere que el efecto básico de la televisión no puede ser cambiado sin cambios correlativos en la organización social.

Nivel de influencia de los medios de comunicación sobre la alimentación saludable en adolescentes de colegios públicos de un distrito de Lima

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Verónica Román

2010-07-01

Full Text Available Introducción: Los medios de comunicación tienen gran influencia en el comportamiento de adolescentes; sus mensajes pueden ser potenciadores o perjudiciales para su salud. Objetivos: Determinar el grado de influencia que ejercen los medios de comunicación tanto en estudiantes pertenecientes a escuelas promotoras para el desarrollo sostenible (EDPS como en aquellos que no pertenecen, así como cuáles son aquellos que más influyen sobre alimentación saludable en estudiantes de nivel secundario. Además, determinar si la condición de EDPS se encuentra asociada a una mayor o menor influencia de los medios de comunicación. Diseño: Estudio observacional, descriptivo y transversal. Lugar: Instituciones Educativas del distrito Puente Piedra. Participantes: Adolescentes de ambos sexos. Intervenciones: Trescientos treinta adolescentes fueron elegidos por conveniencia y distribuidos equitativamente entre pertenecientes y no pertenecientes a EPDS. Cada adolescente respondió un cuestionario sobre la influencia percibida de medios de comunicación acerca de alimentación saludable. Se aplicó chi cuadrado para asociar niveles y Man-Whitney, para comparar puntajes. Principales medidas de resultados: Nivel de influencia percibida de medios de comunicación, cuya puntuación fue: 0 a 8 (bajo, 9 a 16 (medio, 17 a 24 (alto. Resultados: El nivel de influencia ‘medio’ obtuvo el mayor porcentaje en ambos grupos. El promedio de puntaje fue 10,9 para los pertenecientes y 10,6 para los no pertenecientes a EPDS, sobre un máximo de 24 puntos. El medio de comunicación que más influyó en la elección de los alimentos para ambos grupos fue la televisión. El que menos influyó para los pertenecientes fue el diario y para los no pertenecientes, la radio. La familia influenciaría más en la elección de alimentos en los pertenecientes a EPDS y los medios de comunicación en los no pertenecientes. Conclusiones: El nivel de influencia de los medios de

Influence of tumour suppressor gene (TP53, BRCA1 and BRCA2) polymorphisms on polycystic ovary syndrome in South Indian women.

Science.gov (United States)

Siddamalla, Swapna; Reddy, Tumu Venkat; Govatati, Suresh; Guruvaiah, Praveen; Deenadayal, Mamata; Shivaji, Sisinthy; Bhanoori, Manjula

2018-05-24

Polycystic Ovary Syndrome (PCOS) is a heterogeneous multifactorial endocrine metabolic disorder. In addition to hyperandrogenism, acne, hirsutism, obesity, oligoanovulation and infertility, insulin resistance is also a common feature in women of PCOS. Tumor suppressor genes (TSGs) perform essential function in the maintenance of genomic stability and regulatory pathways influencing the activity of several replication and transcription factors. The main aim of this study was to investigate the association of Single Nucleotide Polymorphisms of TP53, BRCA1and BRCA2 genes with the susceptibility to PCOS in South Indian women. Present study investigated association between TP53 gene (rs1042522 G/C), BRCA1 (rs71361504 -/GTT, rs3092986 T/C) and BRCA2 (rs206118 A/G) and, SNPs and PCOS risk. Genotyping of TSGs was carried out on DNA from PCOS patients (n = 110) and controls (n = 130) of South Indian origin by polymerase chain reaction (PCR) and confirmed by sequencing analysis. The genotype frequency and allele distributions of cases and controls were analyzed using Fisher's exact test. Haplotype frequencies for multiple loci and the standardized disequilibrium coefficient (D') for pair wise linkage disequilibrium (LD) were assessed by Haploview Software. Significant increase in frequencies ofTP53 (rs1042522 G/C), BRCA1 (rs71361504 -/GTT, rs3092986 T/C) genotypes and alleles in patients compared to controls. In addition, the frequency of the C/T (P = 0.002) and A/C (P = 0.012) haplotype was also significantly elevated in patients. But BRCA2 (rs206118 A/G) did not show significant association with PCOS. The TP53 and BRCA1 may constitute an inheritable risk factor for PCOS in South Indian women. Copyright © 2018 Elsevier B.V. All rights reserved.

LUNG TUMOR KRAS AND TP53 MUTATIONS IN NON-SMOKERS REFLECT EXPOSURE TO PAH-RICH COAL COMBUSTION EMISSIONS

Science.gov (United States)

Abstract We determined the TP53 and codon 12 KRAS mutations in lung tumors from 24 nonsmokers whose tumors were associated with exposure to smoky coal. Among any tumors studied previously, these showed the highest percentage of mutations that (a) were G -+ T transver...

Influencia de la adición del filler calizo sobre el fraguado del cemento

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Menéndez, Ignacio

1993-09-01

Full Text Available The present paper deals about the infuence that addition of calcareous "filler" has on the set of portland cement which rates are from 0 up to 50% of filler.

En el presente artículo se estudia la influencia que la adición de "filler" calizo ejerce sobre el fraguado del cemento portland, al que se le añaden porcentajes desde O al 50% en filler.

Aging up-regulates ARA55 in stromal cells, inducing androgen-mediated prostate cancer cell proliferation and migration.

Science.gov (United States)

Zou, Qingsong; Cui, Di; Liang, Shengjie; Xia, Shujie; Jing, Yifeng; Han, Bangmin

2016-06-01

Stromal cells in the peripheral zone (PZ) of the prostate from older males (PZ-old) could significantly promote Prostate cancer (PCa) growth compared with stromal cells from young males (PZ-young). But the mechanism is still unknown. In the co-culture system with PZ-old cells, Pc3/Du145 cells showed advanced proliferation and migration after Dihydrotestosterone (DHT) incubation, but DHT didn't show the similar effect in PZ-young co-culture system. Also, higher androgen/AR signal pathway activity and AR-related cytokines secretion (FGF-2, KGF, IGF-1) were found in PZ-old cells. As AR exprssison was equivalent in PZ-old and PZ-young cells, we focused on Androgen receptor associated protein-55(ARA55), a stromal-specific androgen receptor (AR) coactivator. ARA55 expression was higher in PZ-old cells compared with PZ-young cells in vitro. After knocking down ARA55 expression in PZ-old cells, the PCa growth- promoting effect from the PZ-old cells was diminished, which may be explained by the decreased the progressive cytokines secretion (FGF-2, KGF, IGF-1) from PZ-old stromal cells. In vivo, the consistent results were also found: PZ-old cells promoted prostate cancer cells growth, but this effect receded when knocking down ARA55 expression in PZ-old cells. From our study, we found PZ stromal cells presented age-related effects in proliferation and migration of prostate cancer cells in the androgen/AR dependent manner. As aging increased, more ARA55 were expressed in PZ stromal cells, leading to more sensitive androgen/androgen receptor (AR) signal pathway, then constituting a more feasible environment to cancer cells.

Influencia del impuesto hotelero en la red de promoción turística en la capital mexicana

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Tomás Cuevas Contreras

2015-01-01

Full Text Available El presente documento examina la aplicación del impuesto hotelero en la pro-moción del turismo en la capital mexicana, considerando su gestión a través del Fondo Mixto de Promoción Turística de la Ciudad de México mediante la exploración relacional de los actores involucrados. La metodología considera trabajo de gabinete, así como la aproximación cualicuantitativa al fenómeno de estudio. Para ello se exploran diversos enfoques de gestión del gravamen, sus beneficios en la promoción y el soporte al branding del destino. El análisis es mediante visualización de las redes que muestran la interrelación de factores de influencia entre los actores e interesados en la dinámica turística, represen-tando la perspectiva de reticularidad con respecto a la tributación extraordi-naria e influencia en el fomento de la imagen.

Influencia de la autoestima y del apoyo social percibido sobre el bienestar psicológico de estudiantes universitarios chilenos

Directory of Open Access Journals (Sweden)

Enrique Barra Almagia

2012-01-01

Full Text Available El presente estudio, de tipo correlacional transeccional, examinó la influencia de la autoestima y del apoyo social percibido sobre el bienestar psicológico, en una muestra de 450 estudiantes de ambos sexos de la Universidad de Concepción, Chile, quienes respondieron las Escalas de Autoestima de Rosenberg, de Bienestar Psicológico de Ryff y el Cuestionario de Apoyo Social Percibido. Se encontró que la autoestima tenía una influencia mayor sobre el bienestar psicológico que el apoyo social percibido. Aunque no hubo diferencias de sexo en el bienestar psicológico global, se observaron diferencias a favor de los hombres en las dimensiones de autoaceptación y autonomía, y a favor de las mujeres, en crecimiento personal, así como diferencias a favor de los hombres en autoestima y a favor de las mujeres en apoyo socialpercibido.

En búsqueda del ADN y las influencias en algunos sistemas presidenciales y parlamentarios

OpenAIRE

Carpizo, Jorge

2009-01-01

El trabajo examina las recíprocas influencias entre las Constituciones de seis países: tres con sistema presidencial, dos con sistema parlamentario y uno con sistema semipresidencial. Los tres últimos pertenecen a Europa. Los sistemas presidenciales de América Latina se inspiraron en la Constitución Americana, tanto como en alguna europea, especialmente la española de 1812. Los enormes poderes presidenciales en América Latina llevaron a introducir en el sistema preside...

Influencia de las revistas de impacto en el periodismo científico y en la ciencia actual

Directory of Open Access Journals (Sweden)

CARLOS ELÍAS

2002-01-01

Full Text Available En este estudio se ha intentado detectar la influencia de las revistas de impacto, en las que mayoritariamente publican los científicos, en el periodismo especializado y en la ciencia actual. He analizado, sobre todo, el caso español, pero considero que sus conclusiones pueden extenderse al resto de los países. Al margen de que se demuestra la absoluta preponderancia de revistas como Nature y Science (el 45% de la información sobre ciencia de científicos extranjeros que publican los diarios generalistas procede de estas revistas, también se analizan las influencias de la estrategia de comunicación mediática de los gabinetes de prensa de estas publicaciones en la ciencia. Se ha estudiado, especialmente, la preponderancia del idioma inglés y la escasa relevancia del español, así como las consecuencias futuras que tendrá el cada vez mayor impacto de unas pocas revistas en la modificación del método científico en las ciencias experimentales.

¿Tienen los árbitros de fútbol influencia en los resultados de la liga española?

Directory of Open Access Journals (Sweden)

Sala Garrido, Ramon

2009-01-01

Full Text Available RESUMEN El trabajo analiza los resultados de las temporadas 2002-03 a 2007-08 de la Liga Española de Primera División con el fin de determinar la influencia de los árbitros en los mismos, tanto desde el punto de vista del tiempo extra que añaden, como de las sanciones disciplinarias (faltas y tarjetas a los equipos. Se estudia también la influencia que sobre las decisiones del árbitro puede tener la afición del equipo local. Se concluye que no puede afirmarse que la exista influencia arbitral de forma generalizada, aunque algunos árbitros en concreto sí parecen influir en los resultados. El análisis permite también confirmar algo ya sabido, el escaso valor del tiempo extra para cambiar el resultado de un partido.ABSTRACT This paper analyses the results of the 2002-03 to 2007-08 seasons of the First Division Spanish Football League with the aim of determining the influence of referees in them, both from the point of view of the extra time they allow, as well as the disciplinary sanctions (fouls and cards given to the teams. The influence that the supporters of the local team may have over the referee’s decisions is also studied. The conclusion is that it is not possible to confirm the existence of referee influence in a general sense, although some specific referees do seem to have an influence on the results. This analysis also confirms something already known, which is the minimal value of extra time in changing the results of a match.

Clinical Variability in a Family with an Ectodermal Dysplasia Syndrome and a Nonsense Mutation in the TP63 Gene

NARCIS (Netherlands)

Eisenkraft, A.; Pode-Shakked, B.; Goldstein, N.; Shpirer, Z.; Bokhoven, H. van; Anikster, Y.

2015-01-01

Mutations in the TP63 gene have been associated with a variety of ectodermal dysplasia syndromes, among which the clinically overlapping Ankyloblepharon-Ectodermal defects-Cleft lip/palate (AEC) and the Rapp-Hodgkin syndromes. We report a multiplex nonconsanguineous family of Ashkenazi-Jewish

Las organizaciones contemporáneas tipo mp3 y su influencia cultural

Directory of Open Access Journals (Sweden)

Hugo Macías Cardona

2011-06-01

Full Text Available En este artículo se hace una reflexión sobre la influencia cultural de organizaciones contemporáneas con estructuras des-jerarquizadas y procesos internos no secuenciales, que el profesor Richard Sennet ha llamado organizaciones MP3. A partir de las estructuras organizacionales descritas por Álvaro Zapata, la distinción entre trabajo y labor de Hannah Arendt, los mecanismos de vigilancia foucaultianos y una contextualización en Colombia, se analiza la baja lealtad institucional, la disminución de la confianza informal entre quienes laboran y el debilitamiento del conocimiento institucional.

La influencia socioeconómica en el comportamiento de juego de preescolares

OpenAIRE

Pfeifer, Luzia Iara; Rombe, Patrícia Gonçalves; Santos, Jair Licio Ferreira

2009-01-01

Considerando que o contexto econômico influencia o brincar de crianças, este trabalho teve por objetivo analisar as diferenças entre os brinquedos utilizados por crianças de duas diferentes classes socioeconômicas. Os dados foram coletados junto a 50 mães de crianças com idade entre três e seis anos, metade pertencentes à classe econômica A2 (Grupo 2), e a outra metade pertencente à classe econômica D (Grupo 1), segundo o Critério Brasil. Os resultados apontam para a existência de diferenças ...

Steam oxidation of TP 347H FG. Laboratory exposures versus service conditions at the power plant

Energy Technology Data Exchange (ETDEWEB)

Hansson, Anette N. [DONG Energy A/S, Copenhagen (Denmark); Montgomery, Melanie [DONG Energy A/S, Copenhagen (Denmark); Technical Univ. of Denmark, Lyngby (Denmark). Dept. of Mechanical Engineering; Vattenfall Heat Nordic, Copenhagen (Denmark)

2010-07-01

TP347H FG is often used as final superheater tubing at Danish Power Plants. The oxidation behaviour of TP347H FG in steam was investigated both in laboratory conditions and field conditions. Short time exposures (336 hours) were performed in the laboratory at 500, 600 and 700 C in gasses with 8 or 46% H{sub 2}O and varying oxygen partial pressures. The shortest exposure time at the power plant was 7720 h, the temperature varied between 500 and 650 C. Surprisingly, thicker oxide layers formed within the laboratory facility at 600 and 700 C than during the long time exposures at the power plant. This could not be explained by spallation. Double-layered oxides developed during oxidation. The outer layer consist of Fe-oxides and the inner oxide contained Fe and the remaining alloy elements. Investigations with scanning electron microscopy (SEM) revealed that the morphology of the inner oxide was different for the two types of exposures. However, investigation using transmission electron microscopy (TEM) showed that the inner oxide in both cases consisted of particles of Fe-Mn-Cr spinel embedded in a metallic Fe-Ni matrix in the bulk of the (former) alloy grains and Cr-rich oxide layer along the (former) alloy grain boundaries. The main difference between the layers formed at the two locations is that the Cr-rich oxide layer is thicker for the samples exposed at the power plant than that for the samples exposed at the laboratory conditions. Furthermore, the depth of Cr depletion in the alloy adjacent the oxide layer is greater for the samples exposed at the power plant compared to those exposed in the laboratory. The microstructure investigation suggests that the slower oxidation rate of TP347H FG at the power plant as compared to the laboratory is due to a larger reservoir of Cr for the samples exposed at the power plant probably combined with a higher mobility of Cr within the alloy. (orig.)

Influencia de los estilos de supervisión pedagógica en el desempeño docente de la Institución Educativa Primaria Independencia

OpenAIRE

Soncco Quispe, Nancy Elena Esperanza

2013-01-01

La presente investigación cuyo título: "influencia de los estilos de supervisión pedagógica en el desempeño docente de la Institución Educativa Primaria Independencia Nacional de la ciudad de Puno en el año 2013", el objetivo fue de determinar la influencia de los estilos de Supervisión Pedagógica en el Desempeño Docente en dicha Institución, la metodología que se usó son las encuestas de 15 preguntas por variable en consecuencia se obtuvo los siguientes resultados: los estilos de supervisión...

Rare MDM4 gene amplification in colorectal cancer: The principle of a mutually exclusive relationship between MDM alteration and TP53 inactivation is not applicable.

Science.gov (United States)

Suda, Tetsuji; Yoshihara, Mitsuyo; Nakamura, Yoshiyasu; Sekiguchi, Hironobu; Godai, Ten-I; Sugano, Nobuhiro; Tsuchida, Kazuhito; Shiozawa, Manabu; Sakuma, Yuji; Tsuchiya, Eiju; Kameda, Yoichi; Akaike, Makoto; Matsukuma, Shoichi; Miyagi, Yohei

2011-07-01

MDM4, a homolog of MDM2, is considered a key negative regulator of p53. Gene amplification of MDM4 has been identified in a variety of tumors. MDM2 or MDM4 gene amplification is only associated with the wild-type TP53 gene in retinoblastomas, thus the amplification of the two genes is mutually exclusive. Previously, we demonstrated that MDM2 amplification and TP53 alteration were not mutually exclusive in colorectal cancer, and we identified a subset of colorectal cancer patients without alterations in either the TP53 or the MDM2 gene. In this study, we investigated the gene amplification status of MDM4 in the same set of colorectal cancer cases. Unexpectedly, MDM4 amplification was rare, detected in only 1.4% (3 out of 211) of colorectal cancer cases. All the three gene-amplified tumors also harbored TP53-inactivating mutations. This contradicts the simple mutually exclusive relationship observed in retinoblastomas. Surprisingly, two of the three MDM4-amplified tumors also demonstrated MDM2 amplification. Paradoxically, the MDM4 protein levels were decreased in the tumor tissue of the gene-amplified cases compared with levels in the matched normal mucosa. We speculate that MDM4 might play a role in colorectal carcinogenesis that is not limited to negative regulation of p53 in combination with MDM2. The functional significance of MDM4 is still unclear and further studies are needed.

Influencia de la estrategia "toma de decisiones" en el autoconcepto de adolescentes consumidores de alcohol

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Reyna Isabel Hernández Pedroza

2015-12-01

Full Text Available El objetivo de esta investigación fue determinar la influencia de la estrategia "toma de decisiones" en el autoconcepto de adolescentes consumidores de alcohol. Se aplicó un estudio longitudinal, pre-experimental. Se incluyeron 30 adolescentes, previo consentimiento informado de estudiantes y padres. La recolección de datos se realizo con AUDIT, test de conflicto decisional y The Tennessee Self-Concep Scale (TSCS. Se encontró que la edad promedio fue 16.56 ± 0.85 años, 60% hombres, el 100% de los adolescentes presentaron conflicto decisional. El 100% habían consumido alcohol alguna vez en su vida. No se encontró diferencia estadísticamente significativa entre el autoconcepto pre y post intervención (p = 0.27, excepto en la dimensión Yo moral (p = 0.01. Se concluye de acuerdo a los resultados obtenidos en la pre y post intervención que la estrategia de intervención tuvo una influencia en la dimensión del autoconcepto Yo Moral del adolescente consumidor de alcohol. Es posible que esto influyera en la percepción de sí mismo al encontrarse con la realidad de que por primera vez consideraba su capacidad o incapacidad para tomar decisiones.

Fetal colon cell line FHC exhibits tumorigenic phenotype, complex karyotype, and TP53 gene mutation

Czech Academy of Sciences Publication Activity Database

Souček, Karel; Jirsová, Pavla; Brázdová, Marie; Hýžďalová, Martina; Kočí, Lenka; Vydra, D.; Trojanec, R.; Pernicová, Zuzana; Lentvorská, L.; Hajdúch, M.; Hofmanová, Jiřina; Kozubík, Alois

2010-01-01

Roč. 197, č. 2 (2010), s. 107-116 ISSN 0165-4608 R&D Projects: GA MŠk ME 919; GA ČR(CZ) GA204/07/0834; GA ČR(CZ) GA204/08/1560; GA AV ČR(CZ) 1QS500040507; GA MZd NS9600 Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702 Keywords : colon epithelial cells * TP53 * MYC Subject RIV: BO - Biophysics Impact factor: 1.551, year: 2010

En búsqueda del ADN y las influencias en algunos sistemas presidenciales y parlamentarios

OpenAIRE

Jorge Carpizo

2009-01-01

El trabajo examina las recíprocas influencias entre las Constituciones de seis países: tres con sistema presidencial, dos con sistema parlamentario y uno con sistema semipresidencial. Los tres últimos pertenecen a Europa. Los sistemas presidenciales de América Latina se inspiraron en la Constitución Americana, tanto como en alguna europea, especialmente la española de 1812. Los enormes poderes presidenciales en América Latina llevaron a introducir en el sistema presidencial controles propios ...

Influencia de las Emociones Negativas (Ansiedad, Depresión e Ira) sobre la Eficacia de un Programa de Tratamiento Cognitivo Conductual de Deshabituación al Tabaco

OpenAIRE

Francisco Javier Pérez-Pareja; Albert Sesé; Ana Filomena Romo; Alfonso Palmer; Manuel Tomás

2010-01-01

El objetivo de esta investigación es analizar la influencia de la ansiedad, la ira y la depresión, como emociones negativas, sobre el éxito en la aplicación de un tratamiento psicológico de carácter cognitivo-conductual para dejar de fumar. Estas tres respuestas emocionales fueron evaluadas en una muestra final de 180 personas antes de iniciar el tratamiento. Los resultados muestran que la depresión y la ira ejercen una importante influencia sobre el éxito terapéutico; es decir, habiendo deja...

Clinical Variability in a Family with an Ectodermal Dysplasia Syndrome and a Nonsense Mutation in the TP63 Gene.

Science.gov (United States)

Eisenkraft, Arik; Pode-Shakked, Ben; Goldstein, Nurit; Shpirer, Zvi; van Bokhoven, Hans; Anikster, Yair

2015-01-01

Mutations in the TP63 gene have been associated with a variety of ectodermal dysplasia syndromes, among which the clinically overlapping Ankyloblepharon-Ectodermal defects-Cleft lip/palate (AEC) and the Rapp-Hodgkin syndromes. We report a multiplex nonconsanguineous family of Ashkenazi-Jewish descent, in which the index patient presented with a persistent scalp skin lesion, dystrophic nails and light thin hair. Further evaluation revealed over 10 affected individuals in the kindred, over four generations, exhibiting varying degrees of ectodermal involvement. Analysis of the TP63 gene from four of the patients and from two healthy individuals of the same family was performed. Gene sequencing of the patients revealed a nonsense mutation leading to a premature termination codon (PTC) (p.Gln16X). The same mutation was found in all tested affected individuals in the family, but gave rise to marked phenotypic variability with minor clinical manifestations in some individuals, underscoring the clinical heterogeneity associated with the recently described PTC-causing mutations.

Main achievements of the european research project NERIS-TP

International Nuclear Information System (INIS)

Duranova, T.; Bohunova, J.; Raskop, W.; Schneider, T.; Liland, A.; Andronopoulos, S.; Mustonen, R.

2014-01-01

Activities of the NERIS-TP association are described. The NERIS ICRP Workshop, held in Bratislava, Slovak Republic in February 6-8, 2012, was organized by VUJE in cooperation with ICRP aiming to provide a forum for discussion and sharing of experiences on the implementation of the ICRP Recommendations. International, European and national perspectives were presented. 88 specialists from 51 organizations from 26 countries participated in the workshop. The final Dissemination Workshop 'Strengthening the Preparedness at National and Territorial Level Using New Tools and Methods - Stakeholders Experiences', was conducted in Oslo from 22 to 24 January 2014. International organisations such as HERCA, EC DG Research, IAEA, OECD/NEA, and NGOs such as NTW (Nuclear Transparency Watch) and GMF (Group of European Municipalities with Nuclear Facilities) as well as representatives of the OPERRA project participated in the workshop and particular panels. 82 experts and stakeholders participated in the workshop, representing twenty countries. Notably, participants from Japanese organisations provided first feedback from the management of the consequences of the Fukushima accident. (authors)

INFLUENCIA DE LOS INVERSIONISTAS INSTITUCIONALES EN LAS DECISIONES DE ESTRUCTURA DE CAPITAL DE LA EMPRESA. Evidencia para un mercado emergente

Directory of Open Access Journals (Sweden)

Felipe Araya Sepúlveda

2015-01-01

Full Text Available Este artículo analiza empíricamente la influencia de las Administradoras de Fondos de Pensión (AFP como principales accionistas institucionales, sobre las decisiones de estructura de capital de la empresa chilena. Estos inversionistas pueden influir en la estructura de capital mediante distintos papeles como los de monitoreo a la gestión y de recopilación y transferencia de información al mercado. El análisis es desarrollado durante el periodo 2009-2011 para una muestra de 109 empresas chilenas que cotizan en la bolsa. Las AFP no sólo participan en el mercado accionario sino que también compiten por la deuda pública, por lo que nuestros resultados son relevantes al indicar la positiva influencia de este tipo de inversionistas en la contratación y emisión de deuda, particularmente la pública.

A novel splice mutation in the TP53 gene associated with Leydig cell tumor and primitive neuroectodermal tumor

DEFF Research Database (Denmark)

Stecher, Chalotte Willemann; Grønbaek, Kirsten; Hasle, Henrik

2008-01-01

A 20-month-old boy presented with precocious puberty due to a Leydig cell tumor, and at the age of 6 years with a primitive neuroectodermal brain-tumor (PNET). A novel splice site mutation of the TP53-gene, likely to be associated with a nonfunctional protein, was found in the proband, his father...

Influencias nacionales europeas en la política educativa española del siglo XX

Directory of Open Access Journals (Sweden)

Juan Manuel FERNÁNDEZ SORIA

2010-03-01

Full Text Available RESUMEN: En este artículo se estudian las influencias de los macromodelos nacionales europeos en la política educativa española. Francia y su especial influjo en aspectos políticos y administrativos proporciona los principios que atraen a muchos protagonistas de la educación española durante el primer tercio del XX; Alemania, junto a la sistematización de su pensamiento ofrece una pedagogía propiciadora de un socialismo humanista y una ética nueva; el modelo de formación de minorías que caracteriza la pedagogía inglesa fascina a liberales y conservadores. La influencia de la Rusia soviética es visible sobre todo durante la República en guerra, un momento éste en el que, y hasta mediados del siglo, la educación del régimen de Franco conoce también el peso de la pedagogía nazi sobre todo en la organización de la juventud y en experiencias de asistencia social, y de la pedagogía del fascismo italiano en la política educativa del primer franquismo. A partir de entonces la influencia en la política educativa española corresponde a los organismos internacionales.ABSTRACT: The influence European macro models have upon Spanish educational policy, is studied in this article. France and its special influence in political and administrative aspects, provides the principles which attract many protagonists of the Spanish education during the first third of the 20th century. Germany, with the systematisation of its thoughts, offers a pedagogy that propitiates a humanist socialism and a new ethic. The model of education of minorities, characteristic of English pedagogy, fascinates both liberals and conservatives. The influence of the Soviet Russia is visible above all during the Republic in times of the Civil War, moment in which, and until about the middle of the 20th century, Franco’s regime education also knows the weight of nazi pedagogy, specially in youth organisations and in social work experiences, and the Italian’s fascism

Aberrant TP53 detected by combining immunohistochemistry and DNA-FISH improves Barrett's esophagus progression prediction: a prospective follow-up study

NARCIS (Netherlands)

Davelaar, Akueni L.; Calpe, Silvia; Lau, Liana; Timmer, Margriet R.; Visser, Mike; ten Kate, Fiebo J.; Parikh, Kaushal B.; Meijer, Sybren L.; Bergman, Jacques J.; Fockens, Paul; Krishnadath, Kausilia K.

2015-01-01

Barrett's esophagus (BE) goes through a sequence of low grade dysplasia (LGD) and high grade dysplasia (HGD) to esophageal adenocarcinoma (EAC). The current gold standard for BE outcome prediction, histopathological staging, can be unreliable. TP53 abnormalities may serve as prognostic biomarkers.

Aberrant TP53 detected by combining immunohistochemistry and DNA-FISH improves Barrett's esophagus progression prediction : A prospective follow-up study

NARCIS (Netherlands)

Davelaar, Akueni L.; Calpe, Silvia; Lau, Liana; Timmer, Margriet R.; Visser, Mike; ten Kate, Fiebo J.; Parikh, Kaushal B.; Meijer, Sybren L.; Bergman, Jacques J.; Fockens, Paul; Krishnadath, Kausilia K.

2015-01-01

Barrett's esophagus (BE) goes through a sequence of low grade dysplasia (LGD) and high grade dysplasia (HGD) to esophageal adenocarcinoma (EAC). The current gold standard for BE outcome prediction, histopathological staging, can be unreliable. TP53 abnormalities may serve as prognostic biomarkers.

En búsqueda del ADN y las influencias en algunos sistemas presidenciales y parlamentarios

Directory of Open Access Journals (Sweden)

Jorge Carpizo

2009-05-01

Full Text Available El trabajo examina las recíprocas influencias entre las Constituciones de seis países: tres con sistema presidencial, dos con sistema parlamentario y uno con sistema semipresidencial. Los tres últimos pertenecen a Europa. Los sistemas presidenciales de América Latina se inspiraron en la Constitución Americana, tanto como en alguna europea, especialmente la española de 1812. Los enormes poderes presidenciales en América Latina llevaron a introducir en el sistema presidencial controles propios del sistema parlamentario

La danza y su influencia en la percepción de plenitud y bienestar psicoemocional.

OpenAIRE

Rengifo Rodríguez, Lorelcris; Carrero Planes, Virginia

2016-01-01

Introducción: Desde los resultados obtenidos en diversas investigaciones que han identificado la influencia del trabajo corporal sobre la inteligencia emocional y otras variables psicológicas, se derivó el interés por descubrir los efectos o beneficios de la danza sobre las habilidades emocionales u otros procesos psicosociales asociados al bienestar y plenitud. El trabajo que se presenta tiene como objetivo explorar la vivencia de personas que practican danza, de manera profes...

Mutation screening of the TP53 gene by temporal temperature gradient gel electrophoresis.

Science.gov (United States)

Sørlie, Therese; Johnsen, Hilde; Vu, Phuong; Lind, Guro Elisabeth; Lothe, Ragnhild; Børresen-Dale, Anne-Lise

2005-01-01

A protocol for detection of mutations in the TP53 gene using temporal temperature gradient gel electrophoresis (TTGE) is described. TTGE is a mutation detection technique that separates DNA fragments differing by single base pairs according to their melting properties in a denaturing gel. It is based on constant denaturing conditions in the gel combined with a temperature gradient during the electrophoretic run. This method combines some of the advantages of the related techniques denaturing gradient gel electrophoresis (DGGE) and constant denaturant gel electrophoresis (CDGE) and eliminates some of the problems. The result is a rapid and sensitive screening technique that is robust and easily set up in smaller laboratory environments.

Mutation screening of the TP53 gene by temporal temperature gel electrophoresis (TTGE).

Science.gov (United States)

Sørlie, Therese; Johnsen, Hilde; Vu, Phuong; Lind, Guro Elisabeth; Lothe, Ragnhild; Børresen-Dale, Anne-Lise

2014-01-01

A protocol for detection of mutations in the TP53 gene using temporal temperature gradient electrophoresis (TTGE) is described. TTGE is a mutation detection technique that separates DNA fragments differing by single base pairs according to their melting properties in a denaturing gel. It is based on constant denaturing conditions in the gel combined with a temperature gradient during the electrophoretic run. This method combines some of the advantages of the related techniques, denaturing gradient gel electrophoresis and constant denaturant gel electrophoresis, and eliminates some of the problems. The result is a rapid and sensitive screening technique which is robust and easily set up in smaller laboratory environments.

Real-time estimation of TP load in a Mississippi Delta Stream using a dynamic data driven application system

Science.gov (United States)

Ying Ouyang; Theodor D. Leininger; Jeff Hatten

2013-01-01

Elevated phosphorus (P) in surface waters can cause eutrophication of aquatic ecosystems and can impair water for drinking, industry, agriculture, and recreation. Currently, no effort has been devoted to estimating real-time variation and load of total P (TP) in surface waters due to the lack of suitable and/or cost-effective wireless sensors. However, when considering...

Review of prognostic and predictive aspects of mutated TP53 in Wilms’ tumor biology with morphological report and molecular analysis of 37-year-old man’s nephroblastoma

Directory of Open Access Journals (Sweden)

Andrzej Wincewicz

2017-02-01

Full Text Available Here we review prognostic and predictive aspects of mutated TP53 in Wilms’ tumor biology on the basis of the morphological report and molecular analysis of adult nephroblastoma (diffuse blastemal pattern of a 37-year-old man. Among quite different proteins, TP53 affects expression of several genes such as hypoxia inducible proteins GLUT1 and EPO as well as multidrug resistance (MDR mediated by P-glycoprotein (Pgp/MDR1 and multidrug-resistant related protein (MRP1, with certain clinical implications. TP53 mutation was found both in our primary tumor (c.746G>T p.R249M frequency 92% and in nodal metastasis (c.746G>T p.R249M frequency 90%, and the common polymorphism p.P72R in the same gene was revealed with frequency of about 97% in both primary tumor and metastatic disease with appliance of NGS technology (IonTorrent – LifeTechnology using Ion AmpliSeq Cancer Hotspot Panel v2.

Inteligencia emocional y su influencia en el clima organizacional en los niveles gerenciales medios de las PYMES

Directory of Open Access Journals (Sweden)

Cira de Pelekais

2006-05-01

Full Text Available El propósito fue determinar la influencia de la Inteligencia Emocional sobre Clima Organizacional en los niveles gerenciales medios de las PYMES. La investigación fue caracterizada bajo un enfoque descriptivo explicativo, con un diseño no experimental transeccional. Como unidades de análisis fueron tomadas las PYMES adscritas a la cámara de Industria y Comercio de Cabimas, totalizando 100 empresas activas. Para la recolección de la información se utilizó un cuestionario autoadministrado, con escala tipo Likert. Los resultados arrojaron que los gerentes de nivel medio tienen bien identificadas sus emociones y sentimientos, igualmente tienen una excelente percepción del clima organizacional, sin embargo existen ciertos factores de la Inteligencia Emocional que afectan directamente sobre el Clima Organizacional, tal es el caso de la motivación y las habilidades sociales, los cuales no se encuentran en los niveles óptimos de actuación, afectando directamente los niveles de responsabilidad y manejo de conflictos, permitiendo así referenciar que si existe una muy alta influencia de los factores de la Inteligencia Emocional sobre el Clima Organizacional.

Personalidad y trastorno de pánico: un estudio de revisión

OpenAIRE

Osma, Jorge; García-Palacios, Azucena; Botella, Cristina

2014-01-01

A través de este trabajo ofreceremos una revisión exhaustiva de las aportaciones científicas que se han realizado en las últimas tres décadas sobre la influencia de la personalidad en aspectos clínicos y psicopatológicos del Trastorno de pánico (TP), con o sin agorafobia (AG). Los resultados que expondremos serán representativos de dos modelos distintos de evaluación y estudio de la personalidad, el modelo de personalidad clínico y el modelo de personalidad normal. Desde ambas perspectivas, p...

Influencia de las tecnologías celulares en el rendimiento académico

OpenAIRE

y Hellen Navarro Jiménez, Giselle León León, Deyber Alvarado Fernández, Luis Bent Solano

2014-01-01

El presente artículo surge a partir de una investigación sobre la influencia de tecnologías celulares en el rendimiento académico, la cual se realizó bajo el enfoque mixto en la clasificación de mixto dominante, en cuanto a los instrumentos para recopilar la información, se aplicó un cuestionario a dieciséis estudiantes de sétimo año, se realizaron observaciones al desarrollo de las lecciones y se entrevistó al docente de enseñanza de las ciencias de un Liceo del Área Metropolitana costarrice...

Influencia sobre el extractable de níquel de los minerales oxidados del yacimiento Punta Gorda

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Antonio R. Chang-Cardona

2014-12-01

Full Text Available El propósito fue validar, a escala industrial, una metodología para períodos largos en una loza de 8 hornos en la empresa productora de níquel Comandante Ernesto Che Guevara, con el fin de crear una base de datos de producción confiable para su procesamiento estadístico y demostrar la influencia del contenido de los principales minerales oxidados sobre el extractable de níquel y la tendencia en el comportamiento del extractable de cobalto. Se demostró, mediante experimentos, durante tres meses de monitoreo, la efectividad del software COMPRAC para obtener la composición racional de 100 kg de varios tipos de mena laterítica que entran a los hornos de reducción. A partir de la base de datos creada se obtuvo, por primera vez, las gráficas que reflejan la influencia del contenido de los principales minerales sobre los extractables de níquel y de cobalto; en el caso del níquel con suficiente sensibilidad y confiabilidad para la toma de decisiones operacionales eficaces en el circuito tecnológico Mina-Preparación de Mineral-Hornos de Reducción

Influencia del Inglés sobre cuatro lenguas europeas : anglicísmos crudos y semánticos en francés, italiano, neerlandés y rusoi

OpenAIRE

Pino Romero, Francisco Javier del

2013-01-01

En esta tesis se analiza la influencia de la lengua inglesa sobre cuatro lenguas europeas con el fin de entender su evolución desde 1945 hasta nuestros días, conocer la reacción social y gubernamental en los países estudiados, y presentar las buenas prácticas que han funcionado en algunos países en defensa de sus lenguas nacionales. Esta tesis está dividida en cuatro capítulos: francés, italiano, neerlandés y ruso. En cada uno se estudia la evolución de la influencia del inglés, las causa...

RITA (Reactivating p53 and Inducing Tumor Apoptosis) is efficient against TP53abnormal myeloma cells independently of the p53 pathway.

Science.gov (United States)

Surget, Sylvanie; Descamps, Géraldine; Brosseau, Carole; Normant, Vincent; Maïga, Sophie; Gomez-Bougie, Patricia; Gouy-Colin, Nadège; Godon, Catherine; Béné, Marie C; Moreau, Philippe; Le Gouill, Steven; Amiot, Martine; Pellat-Deceunynck, Catherine

2014-06-14

The aim of this study was to evaluate the efficacy of the p53-reactivating drugs RITA and nutlin3a in killing myeloma cells. A large cohort of myeloma cell lines (n = 32) and primary cells (n = 21) was used for this study. This cohort contained cell lines with various TP53 statuses and primary cells with various incidences of deletion of chromosome 17. Apoptosis was evaluated using flow cytometry with Apo2.7 staining of the cell lines or via the loss of the myeloma-specific marker CD138 in primary cells. Apoptosis was further confirmed by the appearance of a subG1 peak and the activation of caspases 3 and 9. Activation of the p53 pathway was monitored using immunoblotting via the expression of the p53 target genes p21, Noxa, Bax and DR5. The involvement of p53 was further studied in 4 different p53-silenced cell lines. Both drugs induced the apoptosis of myeloma cells. The apoptosis that was induced by RITA was not related to the TP53 status of the cell lines or the del17p status of the primary samples (p = 0.52 and p = 0.80, respectively), and RITA did not commonly increase the expression level of p53 or p53 targets (Noxa, p21, Bax or DR5) in sensitive cells. Moreover, silencing of p53 in two TP53(mutated) cell lines failed to inhibit apoptosis that was induced by RITA, which confirmed that RITA-induced apoptosis in myeloma cells was p53 independent. In contrast, apoptosis induced by nutlin3a was directly linked to the TP53 status of the cell lines and primary samples (p RITA, in contrast to nutlin3a, effectively induced apoptosis in a subset of MM cells independently of p53. The findings and could be of interest for patients with a 17p deletion, who are resistant to current therapies.

NSAIDs modulate CDKN2A, TP53, and DNA content risk for progression to esophageal adenocarcinoma.

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Patricia C Galipeau

2007-02-01

Full Text Available Somatic genetic CDKN2A, TP53, and DNA content abnormalities are common in many human cancers and their precursors, including esophageal adenocarcinoma (EA and Barrett's esophagus (BE, conditions for which aspirin and other nonsteroidal anti-inflammatory drugs (NSAIDs have been proposed as possible chemopreventive agents; however, little is known about the ability of a biomarker panel to predict progression to cancer nor how NSAID use may modulate progression. We aimed to evaluate somatic genetic abnormalities with NSAIDs as predictors of EA in a prospective cohort study of patients with BE.Esophageal biopsies from 243 patients with BE were evaluated at baseline for TP53 and CDKN2A (p16 alterations, tetraploidy, and aneuploidy using sequencing; loss of heterozygosity (LOH; methylation-specific PCR; and flow cytometry. At 10 y, all abnormalities, except CDKN2A mutation and methylation, contributed to EA risk significantly by univariate analysis, ranging from 17p LOH (relative risk [RR] = 10.6; 95% confidence interval [CI] 5.2-21.3, p < 0.001 to 9p LOH (RR = 2.6; 95% CI 1.1-6.0, p = 0.03. A panel of abnormalities including 17p LOH, DNA content tetraploidy and aneuploidy, and 9p LOH was the best predictor of EA (RR = 38.7; 95% CI 10.8-138.5, p < 0.001. Patients with no baseline abnormality had a 12% 10-y cumulative EA incidence, whereas patients with 17p LOH, DNA content abnormalities, and 9p LOH had at least a 79.1% 10-y EA incidence. In patients with zero, one, two, or three baseline panel abnormalities, there was a significant trend toward EA risk reduction among NSAID users compared to nonusers (p = 0.01. The strongest protective effect was seen in participants with multiple genetic abnormalities, with NSAID nonusers having an observed 10-y EA risk of 79%, compared to 30% for NSAID users (p < 0.001.A combination of 17p LOH, 9p LOH, and DNA content abnormalities provided better EA risk prediction than any single TP53, CDKN2A, or DNA content

Regulatory Activities of the President of the Office of Electronic Communications versus competitiveness and fi nancial situation of the enterprises on the example of TP Group

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Ewa M. Kwiatkowska

2014-09-01

Full Text Available Purpose: The aim of this paper is to present the regulatory obligations imposed by the President of the Office of Electronic Communications (hereafter: President of UKE on telecom enterprises with significant market power in the retail and wholesale telecommunications markets. Methodology: The decisions addressed to TP Group entities were analyzed as well as the fi nancial statements presenting the economic volume achieved for the period 2006–2013. Findings: In the analyzed period for entities of the TP Group were applied a range of regulatory obligations, that should result in disabling the use of their monopoly position and transfer their position between telecommunications markets. Regulatory obligations imposed by the President of UKE on TP Group entities did not affect to a significant extent the situation of the group. Fluctuations of analyzed financial f gures seem to be primarily the result of internal management decisions rather than a comprehensive range of sector-specific regulation in telecommunications. These researches indicate the need for a deeper analysis of the perception of the effects of regulatory actions by stakeholders in the telecommunications sector.

Identification of TP53 as an Acute Lymphocytic Leukemia Susceptibility Gene Through Exome Sequencing

Science.gov (United States)

Powell, Bradford C.; Jiang, Lichun; Muzny, Donna M.; Treviño, Lisa R.; Dreyer, ZoAnn E.; Strong, Louise C.; Wheeler, David A.; Gibbs, Richard A.; Plon, Sharon E.

2014-01-01

Although acute lymphocytic leukemia (ALL) is the most common childhood cancer, genetic predisposition to ALL remains poorly understood. Whole-exome sequencing was performed in an extended kindred in which five individuals had been diagnosed with leukemia. Analysis revealed a nonsense variant of TP53 which has been previously reported in families with sarcomas and other typical Li Fraumeni syndrome-associated cancers but never in a familial leukemia kindred. This unexpected finding enabled identification of an appropriate sibling bone marrow donor and illustrates that exome sequencing will reveal atypical clinical presentations of even well-studied genes. PMID:23255406

Pyrazolates advance cerium chemistry: a CeIII/CeIV redox equilibrium with benzoquinone.

Science.gov (United States)

Werner, Daniel; Deacon, Glen B; Junk, Peter C; Anwander, Reiner

2017-05-16

Two stable cerium(iv) 3,5-dialkylpyrazolate complexes are presented, namely dimeric [Ce(Me 2 pz) 4 ] 2 (Me 2 pz = 3,5-dimethylpyrazolate) and monomeric Ce(tBu 2 pz) 4 (tBu 2 pz = 3,5-di-tert-butylpyrazolate) along with their trivalent counterparts [Ce(Me 2 pz) 3 ] and [Ce(tBu 2 pz) 3 ] 2 . All complexes were obtained from protonolysis reactions employing the silylamide precursors Ce[N(SiHMe 2 ) 2 ] 4 and Ce[N(SiMe 3 ) 2 ] 3 . Treatment of homoleptic Ce IV and Ce III Me 2 pz complexes with 1,4-hydroquinone (H 2 hq) or 1,4-benzoquinone (bq), respectively, ultimately gave the same trimetallic Ce III species via a cerium redox equilibrium. The Ce III complex Ce 3 (Me 2 pz) 5 (pchd) 2 (L) (pchd = 1,4-bis(3,5-dimethylpyrazol-1-yl)cyclohex-2,5-diene-1,4-diolato; L = Me 2 pzH or (thf) 2 ) results from a di-1,4-pyrazolyl attack on pre-coordinated bq. The reduction of bq by [Ce(Me 2 pz) 3 (thf)] 2 , and re-oxidation by the resulting Ce IV species was supported by UV-vis spectroscopic investigations. Comparisons with the redox-innocent complexes [Ln(Me 2 pz) 3 (thf)] 2 (Ln = La and Pr) revealed far less selective reactions with bq, giving hexametallic and octametallic rare-earth metal side products containing 2-Me 2 pz substituted hq ligands.

Influencia de la poda en el desarrollo de masas de Pinus radiata D. Don y Pinus pinaster Aiton en Asturias

OpenAIRE

Hevia Cabal, Andrea

2013-01-01

Esta tesis evaluó la influencia de diferentes intensidades de poda sobre el crecimiento, desarrollo y persistencia de masas regulares jóvenes de Pinus radiata D. Don y Pinus pinaster Aiton en Asturias, dentro de una selvicultura sostenible enfocada a la producción de madera de calidad.

Influencia del aprendizaje basado en problemas en la actitud ambiental de los estudiantes de la Institución Educativa “José Carlos Mariátegui” Pampachacra Huancavelica 2014

OpenAIRE

Torres Acevedo, Christian Luis

2017-01-01

El título de la investigación fue: Influencia del Aprendizaje Basado en Problemas en la Actitud Ambiental de los estudiantes de la Institución Educativa José Carlos Mariátegui Pampachacra - Huancavelica – 2014. El objetivo: Determinar la influencia de la aplicación del aprendizaje basado en problemas en la actitud ambiental de los estudiantes de la Institución Educativa José Carlos Mariátegui Pampachacra - Huancavelica – 2014. La hipótesis: La aplicación del aprendizaje basado en problemas in...

Acute and persistent Mycobacterium tuberculosis infections depend on the thiol peroxidase TpX.

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Yanmin Hu

Full Text Available The macrophage is the natural niche of Mycobacterium tuberculosis infection. In order to combat oxidative and nitrosative stresses and persist in macrophages successfully, M. tuberculosis is endowed with a very efficient antioxidant complex. Amongst these antioxidant enzymes, TpX is the only one in M. tuberculosis with sequence homology to thiol peroxidase. Previous reports have demonstrated that the M. tuberculosis TpX protein functions as a peroxidase in vitro. It is the dominant antioxidant which protects M. tuberculosis against oxidative and nitrosative stresses. The level of the protein increases in oxidative stress. To determine the roles of tpx gene in M. tuberculosis survival and virulence in vivo, we constructed an M. tuberculosis strain lacking the gene. The characteristics of the mutant were examined in an in vitro stationary phase model, in response to stresses; in murine bone marrow derived macrophages and in an acute and an immune resistant model of murine tuberculosis. The tpx mutant became sensitive to H(2O(2 and NO compared to the wild type strain. Enzymatic analysis using bacterial extracts from the WT and the tpx mutant demonstrated that the mutant contains reduced peroxidase activity. As a result of this, the mutant failed to grow and survive in macrophages. The growth deficiency in macrophages became more pronounced after interferon-gamma activation. In contrast, its growth was significantly restored in the macrophages of inducible nitric oxide synthase (iNOS or NOS2 knockout mice. Moreover, the tpx mutant was impaired in its ability to initiate an acute infection and to maintain a persistent infection. Its virulence was attenuated. Our results demonstrated that tpx is required for M. tuberculosis to deal with oxidative and nitrosative stresses, to survive in macrophages and to establish acute and persistent infections in animal tuberculosis models.

Cambios espaciales y su influencia en la configuración territorial de Aguazul y Tauramena 1974–2005

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Claudia Yolima Devia Acosta

2011-09-01

una periodización basada en el cambio en las actividades económicas y en los cambios político-administrativos en la configuración territorial de estos municipios. El artículo centra el análisis en la influencia de la actividad petrolera en la región, y en su capacidad directa e indirecta de transformar el territorio.

Phosphatidylserine and Phosphatidylethanolamine Bind to Protein Z Cooperatively and with Equal Affinity.

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Tanusree Sengupta

Full Text Available Protein Z (PZ is an anticoagulant that binds with high affinity to Protein Z-dependent protease inhibitor (ZPI and accelerates the rate of ZPI-mediated inhibition of factor Xa (fXa by more than 1000-fold in the presence of Ca2+ and phospholipids. PZ promotion of the ZPI-fXa interaction results from the anchoring of the Gla domain of PZ onto phospholipid surfaces and positioning the bound ZPI in close proximity to the Gla-anchored fXa, forming a ternary complex of PZ/ZPI/fXa. Although interaction of PZ with phospholipid membrane appears to be absolutely crucial for its cofactor activity, little is known about the binding of different phospholipids to PZ. The present study was conceived to understand the interaction of different phospholipids with PZ. Experiments with both soluble lipids and model membranes revealed that PZ binds to phosphatidylserine (PS and phosphatidylethanolamine (PE with equal affinity (Kd~48 μM; further, PS and PE bound to PZ synergistically. Equilibrium dialysis experiments revealed two lipid-binding sites for both PS and PE. PZ binds with weaker affinity to other phospholipids, e.g., phosphatidic acid, phosphatidylglycerol, phosphatidylcholine and binding of these lipids is not synergistic with respect to PS. Both PS and PE -containing membranes supported the formation of a fXa-PZ complex. PZ protection of fXa from antithrombin inhibition were also shown to be comparable in presence of both PS: PC and PE: PC membranes. These findings are particularly important and intriguing since they suggest a special affinity of PZ, in vivo, towards activated platelets, the primary membrane involved in blood coagulation process.

INFLUENCIA DE LA COMPOSICION DE LA LECHE EN POLVO SOBRE LA ACTIVIDAD DE AGUA APLICANDO EL MODELO DE GAB

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Julio Gil

2016-01-01

Full Text Available El presente trabajo, mediante el estudio de las isotermas de sorción a 25ºC, analiza la influencia sobre la actividad de agua (aw, de las diferentes composiciones de leche en polvo entera, descremada y reducida en lactosa. Justamente la incorporación de esta última a trabajos anteriores permite ampliar el comportamiento diferencial de los distintos componentes. E l trabajo permite establecer la relación entre el contenido de humedad de las leches en polvo estudiadas, con su actividad de agua (aw. Los resultados experimentales son evaluados con el modelo referencial de GAB , observando que el trazado de las isotermas y los parámetros del modelo se ajustan satisfactoriamente a los valores obtenidos . Se obser va la clara influencia de la presencia de los monosacáridos (glucosa y galactosa en la leche reducida en lactosa, que disminuyen la actividad a valores mayores a 0 . 4, debido a la mayor solubilidad de estos. El contenido graso en la leche entera, al no ser adsorbente se manifiesta en valores mayores para la actividad de agua.

Microdissecção e captura a laser na investigação do gene TP53 em tecidos incluídos em parafina Laser-capture microdissection for TP53 gene analysis in paraffin-embedded tissues

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Shadia Muhammad Ihlaseh

2007-02-01

Full Text Available INTRODUÇÃO: Microdissecção e captura a laser (MCL é uma técnica de desenvolvimento recente que permite a coleta de células individuais ou pequeno conjunto de células para análise molecular. Atualmente, no Brasil, há raros microscópios para MCL, de modo que a divulgação dos procedimentos inerentes a essa técnica é oportuna para destacar seu amplo potencial para diagnóstico e investigação. OBJETIVO: Este trabalho descreve a padronização dos procedimentos de MCL e de extração de DNA de material fixado em formalina e incluído em parafina. MATERIAL E MÉTODOS: Foram estudados o éxon 8 do gene TP53 e o gene da ciclofilina em amostras de tecido normal e de neoplasias de fígado e rim provenientes de modelo de carcinogênese química induzida em rato. A extração do DNA foi comprovada por reação em cadeia da polimerase (nested-PCR. RESULTADOS: Foram padronizados os procedimentos de preparo dos cortes histológicos, de microdissecção e captura a laser e de obtenção de seqüências gênicas pela reação de nested-PCR para tecidos incluídos em parafina. Obtivemos amplificação de 48,3% das amostras para o éxon 8 do gene TP53 e 51,7% para o gene da ciclofilina. Considerando pelo menos um dos dois segmentos gênicos, foram amplificadas 79,3% das amostras. DISCUSSÃO E CONCLUSÃO: A extração de DNA de tecidos fixados em formalina e incluídos em parafina e a técnica de nested-PCR foram adequadamente padronizadas para produtos gênicos de interesse, obtidos de material coletado por MCL. Esses procedimentos podem ser úteis para a obtenção de seqüências de DNA de arquivos para análise molecular.BACKGORUND: Laser-capture micro-dissection (LCM is a recently developed procedure that provides single cells or specific cell groups for molecular analysis. Currently, there are few LCM systems in Brazil, in such a way that it is necessary to disseminate the technical procedures inherent to the methodology, and also to

Recent (t,p) and (3He,n) studies of elementary excitations in the lead region

International Nuclear Information System (INIS)

Flynn, E.R.

1978-01-01

A number of experiments involving two-nucleon transfer reactions were recently carried out in the region of 208 Pb to explore the limits of the pairing vibration model. ( 3 He,n) studies revealed that the proton pairing elementary excitations give a reasonably simple vibrational picture after corrections for important particle--hole terms. These data are also described accurately by microscopic calculations of the 0 + pairing phonon. (t,p) results on a 204 Hg target reveal a pairing vibrational state in 206 Hg near the predicted energy, after various interactions are taken into account. 14 figures

Evaluation of quality of polaprezinc suppositories and their clinical effect on radiation proctitis

International Nuclear Information System (INIS)

Shikata, Toshiyuki; Nakamura, Takeshi; Hikasa, Shinichi; Hamaguchi, Tsuneo; Doi, Hiroshi; Kamikonya, Norihiko; Kadobayashi, Muneo; Kimura, Takeshi

2010-01-01

Polaprezinc (PZ) suppositories have been used for radiation proctitis in our hospital. In this study, we established methods to evaluate the quality of hospital-prepared PZ suppositories and investigated their stability. As PZ is dissociated at an acidic pH but not at a medium pH, the release of PZ from the suppository was carried out using chelatometric titration in weakly acidic buffer solutions (pH 3, pH 4, and pH 5). As the release of PZ from the suppository was fastest in the buffer solution at pH 3, we used this pH for the release test for PZ. Conducting this test in the stability investigation showed that the amount of PZ released from the suppository after 84 days was the same as that immediately after manufacture. We also examined the clinical efficacy of the PZ suppository in 5 patients with radiation proctitis, finding that efficacy was satisfactory in 3 patients. These results indicated that the PZ suppository is a good treatment for radiation proctitis. (author)

Cobalt(II Complexes with N,N,N-Scorpionates and Bidentate Ligands: Comparison of Hydrotris(3,5-dimethylpyrazol-1-ylborate Tp* vs. Phenyltris(4,4-dimethyloxazolin-2-ylborate ToM to Control the Structural Properties and Reactivities of Cobalt Centers

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Toshiki Nishiura

2018-06-01

Full Text Available Scorpionate ligands Tp* (hydrotris(3,5-dimethylpyrazol-1-ylborate and ToM (tris(4,4-dimethyloxazolin-2-ylphenylborate complexes of cobalt(II with bidentate ligands were synthesized. Both Tp* and ToM coordinate to cobalt(II in a tridentate fashion when the bidentate ligand is the less hindered acetylacetonate. In crystal structures, the geometry of cobalt(II supported by the N3O2 donor set in the Tp* complex is a square-pyramid, whereas that in the ToM complex is close to a trigonal-bipyramid. Both Tp*- and ToM-acac complexes exhibit solvatochromic behavior, although the changing structural equilibria of these complexes in MeCN are quite different. In the bis(1-methylimidazol-2-ylmethylphenylborate (LPh complexes, Tp* retains the tridentate (к3 mode, whereas ToM functions as the bidentate (к2 ligand, giving the tetrahedral cobalt(II complex. The bowl-shaped cavity derived from the six methyl groups on ToM lead to susceptibility to the bulkiness of the opposite bidentate ligand. The entitled scorpionate compounds mediate hydrocarbon oxidation with organic peroxides. Allylic oxidation of cyclohexene occurs mainly on the reaction with tert-butyl hydroperoxide (TBHP, although the catalytic efficiency of the scorpionate ligand complexes is lower than that of Co(OAc2 and Co(acac2. On cyclohexane oxidation with meta-chloroperbenzoic acid (mCPBA, both ToM and Tp* complexes function as catalysts for hydroxylation. The higher electron-donating ToM complexes show faster initial reaction rates compared to the corresponding Tp* complexes.

Association of TP53 codon 72 and CDH1 genetic polymorphisms with colorectal cancer risk in Bangladeshi population.

Science.gov (United States)

Rivu, Sanzana Fareen; Apu, Mohd Nazmul Hasan; Shabnaz, Samia; Nahid, Noor Ahmed; Islam, Md Reazul; Al-Mamun, Mir Md Abdullah; Nahar, Zabun; Rabbi, Sikder Nahidul Islam; Ahmed, Maizbha Uddin; Islam, Mohammad Safiqul; Hasnat, Abul

2017-08-01

Till now no pharmacogenetic study of TP53 codon 72 (Arg72Pro) and CDH1 rs16260 (-160Ccolorectal cancer. So the aim of the study is to determine whether there is an elevated risk of colorectal cancer development with TP53 codon 72 and CDH1 rs16260 genetic polymorphism in Bangladeshi population for the first time. To investigate the association of these two SNPs, we conducted a case-control study with 288 colorectal cancer patients and 295 healthy volunteers by using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method. We found an increased risk of association between Arg/Pro heterozygosity (adjusted OR=2.58, 95% CI=1.77-3.77, pcolorectal cancer predisposition. In case of CDH1 rs16260 polymorphism, C/A heterozygous and A/A mutant homozygous are significantly (pcolorectal cancer risk with adjusted OR of 1.94 and 2.63, respectively. The combined genotype of C/A and A/A was also found to be strongly associated with colorectal cancer risk compared to C/C genotype (adjusted OR=2.02, 95% CI=1.42-2.87, pcolorectal cancer development in Bangladeshi population. Copyright © 2017 Elsevier Ltd. All rights reserved.

IMPROVING THE STUDENTS’ COMPETENCE IN DEVELOPING A PARAGRAPH OF DISCUSSION TEXTS USING THEMATIC PROGRESSION PATTERNS WITH COOPERATIVE LEARNING METHOD (TP-CL

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Farikah Farikah

2017-04-01

Full Text Available This research analyzes the effectiveness of thematic progression patterns with cooperative learning method (TP-CL in teaching the students’ competence in developing a paragraph of discussion texts and the effectiveness of teaching-learning activities of paragraph writing class. This mixed method research was conducted in the English Department of Tidar University of Magelang in the academic year of 2011/2012. The sample of this research were the fourth semester students of UTM. To collect the research data, writing test, observation and interview were used while to analyze the data, she used descriptive and inferential statistics for quantitative data and constant comparative method for qualitative data. The first result shows that implementing TP-CL is effective for teaching the students’ competence in developing a paragraph of discussion texts. It can be seen from the results of writing tests. Based on Brown and Bailey’s theory in Brown (2004, it can be seen that the mean score of organization element in pretest is 10.77 and the post test of paragraph writing is 16.73. The second result shows that there is an improvement of the effectiveness of teaching-learning activity of writing class by implementing TP-CL. The effectiveness can be seen from the indicators of effective teaching based on Blum in Nunan and Lamb (1996 and Creemer’s theory (1994. The results suggest that writing lecturers should introduce to develop a paragraph to the students in order that they can write paragraphs coherently and various theme in order that they can write the paragraph artistically.

Biotransformation of gabapentin in surface water matrices under different redox conditions and the occurrence of one major TP in the aquatic environment.

Science.gov (United States)

Henning, Nina; Kunkel, Uwe; Wick, Arne; Ternes, Thomas A

2018-06-15

Laboratory-scale incubation experiments in water/sediment systems were conducted to test the transformation behavior of the anticonvulsant gabapentin (GBP) under different environmental conditions (aerobic, anaerobic, with abiotic controls). GBP was transformed by biological processes as it was eliminated quickly under aerobic conditions (dissipation time 50% of initial concentration (DT 50 ): 2-7 days) whereas no decrease was observed under anaerobic conditions. Measurements via high resolution mass spectrometry (LC-Orbitrap-MS) revealed eight biological transformation products (TPs). Three of them were identified with reference standards (GBP-Lactam, TP186, TP213), while for the other five TPs tentative structures were proposed from information by MS 2 /MS 3 experiments. Furthermore, the quantitatively most relevant TP GBP-Lactam was formed via intramolecular amidation (up to 18% of initial GBP concentration). Incubation experiments with GBP-Lactam revealed a higher stability against biotic degradation (DT 50 : 12 days) in contrast to GBP, while it was stable under anaerobic and abiotic conditions. Besides GBP, GBP-Lactam was detected in surface water in the μg L -1 range. Finally, GBP and GBP-Lactam were found in potable water with concentrations up to 0.64 and 0.07 μg L -1 , respectively. According to the elevated environmental persistence of GBP-Lactam compared to GBP and its presumed enhanced toxicity, we recommend to involve GBP-Lactam into monitoring programs. Copyright © 2018 Elsevier Ltd. All rights reserved.

Influencia de la salud bucal durante el embarazo en la salud del futuro bebé

OpenAIRE

Díaz Valdés, Liuba; Valle Lizama, Raúl Luis

2015-01-01

Fundamento: El embarazo se relaciona con una mayor incidencia de caries y gingivitis. Objetivo: Profundizar en el conocimiento sobre la influencia de estas afecciones bucales en la salud del futuro bebé. Conclusiones: El embarazo genera adaptaciones en la fisiología femenina las cuales pueden repercutir en la salud bucal de la gestante. Las afecciones bucales más frecuentes son la caries dental y la gingivitis, estas si no son tratadas a tiempo pueden afectar la salud del futuro bebé. Back...

Influencia de la betarraga (Beta vulgaris var. cruenta) en el aumento de leucocitos, en ratones

OpenAIRE

Jony Amaro

2014-01-01

Introducción: La betarraga es una planta oriunda del Mediterráneo utilizada para fines alimenticios y medicinales. Contiene flavonoides. Objetivos: Determinar la influencia del consumo del extracto de Beta vulgaris var. cruenta en el incremento de leucocitos, en el ratón albino. Diseño: Estudio experimental. Lugar: Bioterio de la Institución Educativa 1182. Material biológico: Veinte ratones albinos de la cepa balb/c, machos, de peso promedio 24 g. Intervenciones: Se formó dos grupos, experim...

Mutational analysis of two structural genes of the remperate lactococcal bacteriophage TP901-1 involved in tail length determination and baseplate assembly

DEFF Research Database (Denmark)

Pedersen, Margit; Østergaard, Solvej; Bresciani, José

2000-01-01

Two putative structural genes, orf tmp (tape measure protein) and orf bpp (baseplate protein), of the temperate lactococcal phage TP901-1 were examined by introduction of specific mutations in the prophage strain Lactococcus lactic ssp. cremoris 901-1. The adsorption efficiencies of the mutated...... or duplication of 29% in orf tmp was shown to shorten or lengthen the phage tail by approximately 30%, respectively. The orf tmp is proposed to function as a tape measure protein, TMP, important for assembly of the TP901-1 phage tail and involved in tail length determination. Specific mutations in orf bpp...... produced phages which were unable to adsorb to the indicator strain and electron microscopy revealed particles lacking the baseplate structure. The orf bpp is proposed to encode a highly immunogenic structural baseplate protein, BPP, important for assembly of the baseplate. Finally, an assembly pathway...

Prensa y transición : Cambio 16 y su influencia en la opinión pública (Nov. 1976 - Dic.1977)

OpenAIRE

Santiago Lorenzo, Rosa

2015-01-01

Cambio 16 fue uno de los muchos semanarios y diarios que ayudaron a crear una opinión pública democrática. Se analiza la influencia que tuvo la revista en la opinión pública de la población española

Influencia del advergaming en los jóvenes universitarios de Lima

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Alice Garrido Gallardo

2014-11-01

Full Text Available En tiempos donde el consumidor es capaz de encontrar lo que desea sin ser interrumpido por la publicidad, los anunciantes ven necesaria la tarea de expandir sus mensajes por medio de nuevas alternativas que acerquen al público con la marca de manera atractiva. El advergame forma parte de estas nuevas herramientas ya que busca fidelizar al consumidor mediante juegos interactivos que representen los valores, características y beneficios de la marca. Para conocer cuál es la influencia de esta herramienta publicitaria en los jóvenes de la ciudad de Lima, este artículo realiza un análisis sobre la actitud que ellos generan hacia la marca dependiendo del tratamiento que se le da en el juego, el tiempo de exposición y otras variables.

Cuatro indicios de la influencia de la Declaración universal de los derechos humanos de 1948 en el constitucionalismo Colombiano

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Juan Carlos Upegui Mejía

2009-12-01

Full Text Available El autor desarrolla la tesis de la influencia de la Declaración Universal de los Derechos Humanos de 1948 en el constitucionalismo colombiano a partir de la identificación de cuatro indicios: 1. el conocimiento y la invocación de la Declaración Universal por parte de varios delegatarios a la Asamblea Constituyente de 1991; 2. algunas cifras sobre la invocación de la Declaración Universal durante los primeros 16 años de funcionamiento de la Corte Constitucional colombiana; 3. algunos datos cualitativos sobre la influencia de la Declaración Universal en la concepción de los derechos fundamentales de la Corte Constitucional colombiana; y, finalmente, 4. una breve reseña de algunos manuales de derecho constitucional, donde se indica la forma en que estos han o no acogido los contenidos de la Declaración, así como la relación de esta última con el derecho interno.

Influencia del yeso sobre la velocidad de hidratación del cemento portland

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Yamaguchi, G.

1961-12-01

Full Text Available Not availablePara esclarecer la Influencia del yeso sobre el fraguado y endurecimiento del cemento, los autores estudiaron el grado de hidratación de los cuatro principales minerales del clínker de cemento Portland y el efecto del yeso sobre ellas. Haciendo uso del análisis cuantitativo de rayos X, se determinó la porción no hidratada. Simultáneamente, se determinaron los tiempos de fraguado y las resistencias. Los ensayos se llevaron a cabo sobre tres clínkeres sintéticos de diferentes composiciones y sobre dos clínkeres de cemento Portland comerciales.

Influencia de la rodilla en la amortiguación de vibraciones sobre plataforma oscilante

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Alejandro Bruñó-Soler

2014-01-01

Full Text Available La utilización de plataformas vibratorias se ha extendido en los ámbitos deportivo y clínico. Sin embargo, son escasos los trabajos sobre su correcta utilización y seguridad. En este estudio transversal se registró, en 22 sujetos sanos (23,7 ± 3,7 años, talla 1,75 ± 0,08 m, masa 71,63 ± 14,5 kg con un nivel de actividad física bajo/medio, la aceleración en el eje vertical en la cabeza durante la posición de bipedestación sobre una plataforma vibratoria, en tres angulaciones distintas (180º, 150º y 120º, a tres frecuencias de vibración (5, 16 y 27 Hz y a una amplitud constante (3 mm. El objetivo del estudio fue valorar la influencia de la posición de la rodilla sobre la transmisión de vibraciones desde la plataforma hasta la cabeza. Los resultados indican que la amortiguación de la vibración aumentó con cada incremento en el grado de flexión de la rodilla, siendo la influencia de la posición articular tanto más relevante cuanto mayor fue la frecuencia de vibración de la plataforma (p <0.001. Estos resultados sugieren que para la realización segura de ejercicios en bipedestación sobre plataforma vibratoria a intensidades altas debe evitarse la extensión completa de rodilla.

潜伏性热释放2PZ-PS-co-MAA微胶囊固化剂的制备与性能%Preparation and Performance of Heat Released 2PZ-PS-co-MAA Mierocapsule-Type Latent Curing Agent

Institute of Scientific and Technical Information of China (English)

史有强; 张秋禹; 陈少杰; 马明亮; 马爱洁; 顾军渭

2012-01-01

以2-苯基咪唑（2PZ）为芯材,苯乙烯-甲基丙烯酸共聚物（PS-co-MAA）为壁材,采用溶剂挥发技术,成功地制备了一种新型潜伏性热释放2PZ-PS-co-MAA微胶囊固化剂。通过红外光谱仪（FT-IR）、热重分析仪（TGA）、扫描电子显微镜（SEM）、粒度分析仪和差示扫描量热仪（DSC）对微胶囊固化剂的化学结构、芯材含量、表面形貌、粒径分布及固化性能等进行了表征。所制备的微胶囊固化剂表面光滑,粒径分布较窄,平均粒径约为15.60μm,壁材厚度约为0.5μm,芯材2PZ含量约为39.19%。由微胶囊固化剂与环氧树脂E-51制备的单组分胶粘剂,具有优良的固化特性和潜伏性能,可在100℃,30 min内实现固化,室温储存期可达32 d以上。%A heat released microcapsule-type latent curing agent was successfully prepared by solvent evaporation technique with 2-phenylimidazole（2PZ） as the core material and styrene/methacrylic acid copolymer（PS-co-MAA） as the wall material.The chemical structure,core material content,surface morphology,size distribution and curing characteristics of this microcapsule-type curing agent were characterized by Fourier transform infrared spectrum（FT-IR）,thermogravimetric analysis（TGA）,scanning electron microscope（SEM）,granulometer and differential scanning calorimetry（DSC）.The obtained microcapsules have a smooth surface and display a narrow size distribution with the mean size about 15.60 μm,and its wall thickness is about 0.5 μm with core material content about 39.19%.In addition,the one-component adhesive made from the microcapsules and epoxy resin E-51 shows advanced curing characteristics and latent properties.It is found that the E-51/PS-co-MAA microcapsule system can be cured at 100 ℃ in 30 min and its shelf life at room temperature is more than 32 days.

4-Pyridone-3-carboxamide-1-β-d-ribonucleoside Triphosphate (4PyTP, a Novel NAD+ Metabolite Accumulating in Erythrocytes of Uremic Children: A Biomarker for a Toxic NAD+ Analogue in Other Tissues?

Directory of Open Access Journals (Sweden)

Elizabeth A. Carrey

2011-06-01

Full Text Available We have identified a novel nucleotide, 4-pyridone 3/5-carboxamide ribonucleoside triphosphate (4PyTP, which accumulates in human erythrocytes during renal failure. Using plasma and erythrocyte extracts obtained from children with chronic renal failure we show that the concentration of 4PyTP is increased, as well as other soluble NAD+ metabolites (nicotinamide, N1-methylnicotinamide and 4Py-riboside and the major nicotinamide metabolite N1-methyl-2-pyridone-5-carboxamide (2PY, with increasing degrees of renal failure. We noted that 2PY concentration was highest in the plasma of haemodialysis patients, while 4PyTP was highest in erythrocytes of children undergoing peritoneal dialysis: its concentration correlated closely with 4Py-riboside, an authentic precursor of 4PyTP, in the plasma. In the dialysis patients, GTP concentration was elevated: similar accumulation was noted previously, as a paradoxical effect in erythrocytes during treatment with immunosuppressants such as ribavirin and mycophenolate mofetil, which deplete GTP through inhibition of IMP dehydrogenase in nucleated cells such as lymphocytes. We predict that 4Py-riboside and 4Py-nucleotides bind to this enzyme and alter its activity. The enzymes that regenerate NAD+ from nicotinamide riboside also convert the drugs tiazofurin and benzamide riboside into NAD+ analogues that inhibit IMP dehydrogenase more effectively than the related ribosides: we therefore propose that the accumulation of 4PyTP in erythrocytes during renal failure is a marker for the accumulation of a related toxic NAD+ analogue that inhibits IMP dehydrogenase in other cells.

La influencia de las guerras civiles sobre la represión estatal en posguerras

OpenAIRE

Herreros, Francisco

2014-01-01

En este artículo se analizan los determinantes de la represión estatal en posguerras. Su argumento principal es que ciertas dinámicas de las guerras civiles explican la variación en represión estatal en la posguerra. Para comprobar la influencia de las guerras civiles en la represión estatal, se emplea una base de datos que incluye a todos los países que han experimentado una guerra civil en el período 1976-2009. Los resultados muestran que una victoria rebelde en la guerra o un compromiso en...

Cubierta vegetal frente a laboreo en dos zonas con distinta disponibilidad hídrica: influencia sobre producción, vigor, calidad y estado hídrico de la vid

OpenAIRE

Díez Bueno, Nuria

2012-01-01

El objetivo de este trabajo es evaluar la influencia de una cubierta vegetal frente al laboreo convencional en dos tipos de suelo con diferente dotación hídrica (suelo de pendiente con riego y suelo de vaguada sin riego) y su influencia sobre el estado hídrico de la planta, la producción, el vigor y la calidad. El presente trabajo final de carrera se realizó en una parcela de viñedo plantado en 1980 de la variedad Tempranillo injertado sobre patrón 41B, de conducción en espaldera, de c...

Contribution of the TP53 R337H mutation to the cancer burden in southern Brazil: Insights from the study of 55 families of children with adrenocortical tumors.

Science.gov (United States)

Mastellaro, Maria J; Seidinger, Ana L; Kang, Guolian; Abrahão, Renata; Miranda, Eliana C M; Pounds, Stanley B; Cardinalli, Izilda A; Aguiar, Simone S; Figueiredo, Bonald C; Rodriguez-Galindo, Carlos; Brandalise, Silvia R; Yunes, José A; Barros-Filho, Antônio de A; Ribeiro, Raul C

2017-08-15

The tumor protein p53 (TP53) arginine-to-histidine mutation at codon 337 (R337H) predisposes children to adrenocortical tumors (ACTs) and, rarely, to other childhood tumors, but its impact on adult cancer remains undetermined. The objective of this study was to investigate the frequency and types of cancer in relatives of children with ACT who carry the TP53 R337H mutation. TP53 R337H testing was offered to relatives of probands with ACT. The parental lineage segregating the R337H mutation was identified in all families. The frequency and distribution of cancer types were compared according to R337H status. The authors' data also were compared with those publicly available for children with TP53 mutations other than R337H. The mean and median follow-up times for the probands with ACT were 11.2 years and 9.7 years (range, 3-32 years), respectively. During this time, cancer was diagnosed in 12 of 81 first-degree relatives (14.8%) carrying the R337H mutation but in only 1 of 94 noncarriers (1.1%; P = .0022). At age 45 years, the cumulative risk of cancer was 21% (95% confidence interval, 5%-33%) in carriers and 2% (95% confidence interval, 0%-4%) in noncarriers (P = .008). The frequency of cancer was higher in the R337H segregating lineages than in the nonsegregating lineages (249 of 1410 vs 66 of 984 individuals; P cancer were the most common types. TP53 R337H carriers have a lifelong predisposition to cancer with a bimodal age distribution: 1 peak, represented by ACT, occurs in the first decade of life, and another peak of diverse cancer types occurs in the fifth decade. The current findings have implications for genetic counseling and surveillance of R337H carriers. Cancer 2017;123:3150-58. © 2017 American Cancer Society. © 2017 American Cancer Society.

Transportability Testing of the Family of Medium Tactical Vehicles (FMTV) 10-Ton Dump Truck, TP-94-01, Rev. 2, June 2004, "Transportability Testing Procedures"

National Research Council Canada - National Science Library

Barickman, Philip W

2007-01-01

... (FMTV) 10-ton dump truck manufactured by Stewart and Stevenson Systems, Inc., Sealy, Texas. The testing was conducted in accordance with TP-94-01, Revision 2, June 2004 "Transportability Testing Procedures...

Sex reversal in zebrafish fancl mutants is caused by Tp53-mediated germ cell apoptosis.

Directory of Open Access Journals (Sweden)

Adriana Rodríguez-Marí

2010-07-01

Full Text Available The molecular genetic mechanisms of sex determination are not known for most vertebrates, including zebrafish. We identified a mutation in the zebrafish fancl gene that causes homozygous mutants to develop as fertile males due to female-to-male sex reversal. Fancl is a member of the Fanconi Anemia/BRCA DNA repair pathway. Experiments showed that zebrafish fancl was expressed in developing germ cells in bipotential gonads at the critical time of sexual fate determination. Caspase-3 immunoassays revealed increased germ cell apoptosis in fancl mutants that compromised oocyte survival. In the absence of oocytes surviving through meiosis, somatic cells of mutant gonads did not maintain expression of the ovary gene cyp19a1a and did not down-regulate expression of the early testis gene amh; consequently, gonads masculinized and became testes. Remarkably, results showed that the introduction of a tp53 (p53 mutation into fancl mutants rescued the sex-reversal phenotype by reducing germ cell apoptosis and, thus, allowed fancl mutants to become fertile females. Our results show that Fancl function is not essential for spermatogonia and oogonia to become sperm or mature oocytes, but instead suggest that Fancl function is involved in the survival of developing oocytes through meiosis. This work reveals that Tp53-mediated germ cell apoptosis induces sex reversal after the mutation of a DNA-repair pathway gene by compromising the survival of oocytes and suggests the existence of an oocyte-derived signal that biases gonad fate towards the female developmental pathway and thereby controls zebrafish sex determination.

Plio-Pleistocene landscape and vegetation reconstruction of the coastal area of the Tjörnes Peninsula, Northern Iceland

DEFF Research Database (Denmark)

Verhoeven, Koen; Louwye, Stephen; Eiriksson, Jón

2013-01-01

Marine and continental deposits from the Tjornes area in northern Iceland were studied to obtain their pollen/spore content. Six Pollen Zones (PZ) were defined in the Early Pliocene Tjornes beds and the Early Pleistocene Breidavik Group. The pollen is most diverse during the deposition of the low......Marine and continental deposits from the Tjornes area in northern Iceland were studied to obtain their pollen/spore content. Six Pollen Zones (PZ) were defined in the Early Pliocene Tjornes beds and the Early Pleistocene Breidavik Group. The pollen is most diverse during the deposition...... of the lowest Tapes Zone (PZ 1) and the lower part of the overlying Mactra Zone (PZ 2). Local pollen from marshland, levee and foothill forests was deposited on a large coastal plain. The pollen spectrum reflects transgression and deepening during the second part of the Mactra Zone (PZ 3) and the lower part...... of the Serripes Zone (PZ 4). Gymnosperm pollen derived from the higher inland plateau increases in PZ 3. This background pollen was of minor importance during periods with an extensive coastal plain (PZ 1, 2, 4, 6). PZ 5 did not yield sufficient pollen for analysis. The pollen analysis allowed refinement...

Corrosion inhibition properties of pyrazolylindolenine compounds on copper surface in acidic media

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Ebadi Mehdi

2012-12-01

Full Text Available Abstract Background The corrosion inhibition performance of pyrazolylindolenine compounds, namely 4-(3,3-dimethyl-3H-indol-2-yl-pyrazole-1-carbothioamide (InPzTAm, 4-(3,3-dimethyl-3H-indol-2-yl-1H-pyrazole-1-carbothiohydrazide (InPzTH and 3,3-dimethyl-2-(1-phenyl-1H-pyrazol-4-yl-3H-indole (InPzPh, on copper in 1M HCl solution is investigated by electrochemical impedance spectroscopy (EIS, open circuit potential (OCP and linear scan voltammetry (LSV techniques. Results The results show that the corrosion rate of copper is diminished by the compounds with the inhibition strength in the order of: InPzTAm> InPzTH > InPzPh. The corrosion inhibition efficiencies for the three inhibitors are 94.0, 91.4 and 79.3, for InPzTAm, InPzTH and InPzPh respectively with the same inhibitor concentration (2 mM. Conclusion From the EIS, OCP and LSV results it was concluded that pyrazolylindolenine compounds with S-atom (with an amine group have illustrated better corrosion inhibition performance compared to hydrazine and phenyl group.

Influencia de la humedad en el ensayo de resistencia de los ladrillos de suelo-cemento

OpenAIRE

Lima, José I.; Escobar Martín, Dunia

1994-01-01

A través de un diseño experimental adecuado se pretende mostrar la influencia de la humedad en el ensayo a compresión de los ladrillos de suelo-cemento, obteniendo las ecuaciones que muestran esta relación. Su generalización posterior permitió elaborar una matriz de correlación de las resistencias con las diferentes humedades de ensayo, de forma tal que se pueda ensayar con una humedad dada y estimar la resistencia que tendrá esa muestra para diferentes valores de humedad, con un buen nive...

INFLUENCIA DEL LIDERAZGO DOCENTE EN LA EFECTIVIDAD DE LA ESCUELA DE POSGRADO DE LA UNE

OpenAIRE

Huerta Camones, Luis Eustaquio; Universidad Nacional Agraria La Molina (Perú).

2016-01-01

Esta investigación tuvo como propósito determinar la influencia del liderazgo que los docentes ejercen en las aulas sobre la efectividad de la escuela de posgrado de la UNE. Se basó en la teoría de liderazgo transformacional. Se usaron una muestra de 126 estudiantes, instrumentos adaptados y la regresión lineal múltiple. Los resultados más importantes encontrados fueron que el liderazgo docente influye positiva y significativamente en la efectividad de la escuela de posgrado de la UNE y que e...

Mutational profile and prognostic significance of TP53 in diffuse large B-cell lymphoma patients treated with R-CHOP

DEFF Research Database (Denmark)

Xu-Monette, Zijun Y; Wu, Lin; Visco, Carlo

2012-01-01

TP53 mutation is an independent marker of poor prognosis in patients with diffuse large B-cell lymphoma (DLBCL) treated with cyclophosphamide, hydroxydaunorubicin, vincristine, and prednisone (CHOP) therapy. However, its prognostic value in the rituximab immunochemotherapy era remains undefined. ...... for stratifying R-CHOP-treated patients into distinct prognostic subsets and has significant value in the design of future therapeutic strategies....

Celos y violencia en alumnos de enseñanza secundaria. Influencia de los estilos educativos familiares

OpenAIRE

Aymerich Navarro, Mª del Milagro

2016-01-01

El objetivo de la tesis consiste en establecer la relación entre los celos en la adolescencia y la influencia de los estilos educativos familiares a la hora de explicar la violencia entre iguales, las reacciones emocionales y las conductas desajustadas de los adolescentes. Las reacciones emocionales se miden con las variables: celos, ansiedad y hostilidad. En cuanto a las reacciones conductuales se maneja como variable el bullying o victimización entre iguales. Con respecto a la variable e...

Reduced rate of human papillomavirus infection and genetic overtransmission of TP53 72C polymorphic variant lower cervical cancer incidence.

Science.gov (United States)

Alsbeih, Ghazi A; Al-Harbi, Najla M; Bin Judia, Sara S; Khoja, Hatim A; Shoukri, Mohamed M; Tulbah, Asma M

2017-07-01

Cervical cancer is a predominantly human papillomavirus (HPV)-driven disease worldwide. However, its incidence is unexplainably low in western Asia, including Saudi Arabia. Using this paradigm, we investigated the role of HPV infection rate and host genetic predisposition in TP53 G72C single nucleotide polymorphism (SNP) presumed to affect cancer incidence. Patients treated between 1990 and 2012 were reviewed, and a series of 232 invasive cervical cancer cases were studied and compared with 313 matched controls without cancer. SNP was genotyped by way of direct sequencing. HPV linear array analysis was used to detect and genotype HPV in tumor samples. The incidence of cervical cancer revealed bimodal peaks at 42.5 years, with a slighter rebound at 60.8 years. Among all cases, 77% were HPV-positive and 16 HPV genotypes were detected-mostly genotypes 16 (75%) and 18 (9%)-with no difference by age, histology, or geographical region. Although the TP53 G72C genotype was not associated with overall cervical cancer risk, it was significantly associated with HPV positivity (odds ratio, 0.57; 95% confidence interval, 0.36-0.90; P = .016). Furthermore, the variant C allele was significantly overtransmitted in the population (P Cervical cancer incidence displays bimodal curve peaking at a young age with secondary rebound at older age. The combination of relative low HPV infection and variant TP53 72C allele overtransmission provide a plausible explanation for the low incidence of cervical cancer in our population. Therefore, HPV screening and host SNP genotyping may provide more relevant biomarkers to gauge the risk of developing cervical cancer. Cancer 2017;123:2459-66. © 2017 American Cancer Society. © 2017 The Authors. Cancer published by Wiley Periodicals, Inc. on behalf of American Cancer Society.

Influencia de 46 porta-injertos para cítricos en la precocidad o retardo de maduración de la naranja "Frost valencia" (Citrus sinensis (L. Osbeck

Directory of Open Access Journals (Sweden)

Caselles N. Álvaro A.

1987-03-01

Full Text Available Of 46 graft carrier influence in citrics on ripe precocity or retardation from "Frost Valencia" Citrus sinensis (L Osbeck was studied looking for to guaranter a fruit constant supply toward market. Graft carrier influence was tested in orange blosson, fruiting ripeness season. Stock influence to induce early intermediate or later blossoms was observed, related with period and intensity so them same ocurr. Stock influence on solubles solids content, juici volume, ripeness index, ripe fruit, mantenence on tree also was found . Early of later yields are feasible to obtain using trifoliado x Ruby Or (1437 and "Garcia Valencia" stock markedly, or which highly significant ripeness gains were found with "Frost Valencia" Orange respectively.Se estudió la influencia de los portainjertos para cítricos buscando la obtención de producciones tempranas o tardías para garantizar un suministro continuo de fruta fresca al mercado. Se analizó por espacio de dos años la influencia de los porta-injertos en el período de floración, fructificación y maduración de la naranja. Se observó influencia del patrón para inducir floraciones precoces, intermedias o tardías haciendo referencia al período de intensidad con que ocurren las mismas. También se encontró influencia del patrón en contenido de sólidos solubles, volumen de jugo, acidez, índice de marez y mantenimiento de la fruta madura en el árbol. Es posible obtener producciones tempranas o tardías con la utilización de los patrones trifoliados x Ruby Or (1437 Y García Valencia, sobre las cuales se encontró adelantos altamente significativos de maduración de la naranja "Frost Valencia" respectivamente.

Mutational pattern of TP53 tumor suppressor gene in human lung cells exposed to air pollution PM{sub 2.5}; Spectre mutationnel de TP53 en reponse a une exposition in vitro a un aerosol atmospherique particulaire PM{sub 2,} {sub 5}

Energy Technology Data Exchange (ETDEWEB)

Billet, Sylvain; Paget, Vincent [Universite Lille Nord de France, Lille (France); Unite de Chimie Environnementale et Interactions sur le Vivant, Maison de la Recherche en Environnement Industriel, Universite du Littoral Cote d' Opale, Dunkerque (France); GRECAN, Universite de Caen Basse-Normandie et Centre Regional de Lutte Contre le Cancer Francois Baclesse, Caen (France); Garcon, Guillaume; Verdin, Anthony; Shirali, Pirouz [Universite Lille Nord de France, Lille (France); Unite de Chimie Environnementale et Interactions sur le Vivant, Maison de la Recherche en Environnement Industriel, Universite du Littoral Cote d' Opale, Dunkerque (France); Andre, Veronique; Heutte, Natacha; Sichel, Francois [GRECAN, Universite de Caen Basse-Normandie et Centre Regional de Lutte Contre le Cancer Francois Baclesse, Caen (France)

2012-01-15

Environmental exposure to fine airborne particulate matter (PM 2.5) is thought to be responsible for cardiopulmonary diseases, including lung cancer. However, the mechanisms of action potentially involved in PM{sub 2.5} toxicity are not yet fully described. Mutations in the TP53 gene are the most common alterations in human solid tumors. TP53 mutational patterns have sometimes been linked to carcinogen exposure. The purpose of this study was to determine the mutations that alter the functionality of this transcription factor in a model of human epithelial lung cells (A549) exposed to the fine particulate fraction (PM{sub 2.5}) of an atmospheric aerosol sampled under urban and industrial influences. PM{sub 2.5} was collected in Dunkerque City by cascade impaction. Its physicochemical characterization revealed the presence of many inorganic and organic compounds, including some that are known for their toxicity. The search for mutations altering the functionality of the P53 protein was performed 72 h after exposure of A549 cells to PM{sub 2.5} at its lethal concentration at 50% (LC{sub 50}, 118.60 {mu}g/mL = 31.63 {mu}g/cm{sup 2}), using the Functional Analysis of Separated Alleles in Yeast (FASAY). Sixteen mutations altering P53 function were detected after A549 cells exposure to the collected PM{sub 2.5}: eight deletions of one or two nucleotides and eight nucleotide substitutions, mainly transitions A > G and G > A. These mutations are described in the literature as possibly caused by endogenous mechanisms, such as oxidative stress. This kind of alteration can be induced by metal content of the PM{sub 2.5}, as well as by metabolic activation of the organic compounds coated onto its surface. Involvement of oxidative stress in TP53 mutations was confirmed by the detection of an oxidative DNA adduct, 8-hydroxy-2'-deoxyguanosine (8-OHdG), in A549 cells exposed to the collected PM. (authors)

Influencia del clima escolar y familiar en adolescentes, víctimas de ciberacoso

Directory of Open Access Journals (Sweden)

Jessica Ortega Barón

2016-01-01

Full Text Available El ciberacoso es un fenómeno de creciente preocupación social que afecta cada vez más a niños y adolescentes de todos los países desarrollados. A diferencia de la considerable literatura que hay sobre las relaciones entre el acoso escolar y el contexto familiar y escolar, todavía hay pocos trabajos sobre la influencia de estos entornos sociales en el problema del ciberacoso. Mediante una metodología cuantitativa, el objetivo principal del presente estudio fue analizar la influencia del contexto escolar y familiar en víctimas de ciberacoso. La muestra estuvo formada por 1.062 adolescentes (51,5% chicos y 48,5% chicas, de edades comprendidas entre los 12 y los 18 años (M=14,5; DT=1,62. Se establecieron tres grupos de contraste: cibervíctimas severas, cibervíctimas moderadas y no víctimas de ciberacoso. Los resultados del análisis de varianza indicaron que las cibervíctimas severas en comparación con las no víctimas puntúan significativamente más alto en conflicto familiar y obtienen puntuaciones más bajas en el resto de variables familiares (autoestima familiar, cohesión y expresividad, y variables escolares (implicación, afiliación y ayuda al profesor, consideradas en el estudio. Los análisis de regresión revelaron que la autoestima académica y familiar y algunas dimensiones del clima familiar y escolar predicen la cibervictimización en la adolescencia. Estos novedosos resultados muestran la importancia de incluir a la familia y a la escuela en los programas de prevención del ciberacoso.

Influencia de los cementos y de los diseños protésicos en el ajuste interno de estructuras de zirconia confeccionadas mediante tecnología Cad-Cam para prótesis fija

OpenAIRE

Sánchez Monescillo, Andrés

2015-01-01

1. INTRODUCCIÓN Y JUSTIFICACIÓN Aún quedan gran cantidad de elementos y variables que pueden influir en el ajuste interno de las restauraciones y cuyo desconocimiento obliga a seguir investigando. Este estudio permite conocer si existe una influencia real en el ajuste interno de estructuras de zirconia fabricadas mediante tecnología CAD-CAM en función del tipo de cemento utilizado y del tipo de convergencia oclusal de la preparación realizada. 2. OBJETIVOS - Evaluar la influencia de disti...

Analysis of the roles of E6 binding to E6TP1 and nuclear localization in the human papillomavirus type 31 life cycle

International Nuclear Information System (INIS)

Lee, Choongho; Wooldridge, Tonia R.; Laimins, Laimonis A.

2007-01-01

The E6 oncoproteins of high-risk human papillomaviruses provide important functions not only for malignant transformation but also in the productive viral life cycle. E6 proteins have been shown to bind to a number of cellular factors, but only a limited number of analyses have investigated the effects of these interactions on the viral life cycle. In this study, we investigated the consequences of HPV 31 E6 binding to E6TP1, a putative Rap1 GAP protein. HPV 16 E6 has been shown to bind as well as induce the rapid turnover of E6TP1, and similar effects were observed with HPV 31 E6. Mutation of amino acid 128 in HPV 31 E6 was found to abrogate the ability to bind and degrade E6TP1 but did not alter binding to another α-helical domain protein, E6AP. When HPV 31 genomes containing mutations at amino acid 128 were transfected into human keratinocytes, the viral DNAs were not stably maintained as episomes indicating the importance of this residue for pathogenesis. Many E6 binding partners including E6TP1 are cytoplasmic proteins, but E6 has been also reported to be localized to the nucleus. We therefore investigated the importance of E6 localization to the nucleus in the viral life cycle. Using a fusion of E6 to Green Fluorescent Protein, we mapped one component of the nuclear localization sequences to residues 121 to 124 of HPV 31 E6. Mutation of these residues in the context of the HPV 31 genome abrogated the ability for episomes to be stably maintained and impaired the ability to extend the life span of cells. These studies identify two activities of HPV 31 E6 that are important for its function in the viral life cycle and for extension of cell life span

La influencia de la ideología política y la intolerancia a la ambigüedad sobre diversas expresiones actitudinales del heterosexismo

Directory of Open Access Journals (Sweden)

Jan Marc Rottenbacher de Rojas

2015-01-01

Full Text Available El estudio analiza la influencia que ejercen la intolerancia a la ambigüedad y el conservadurismo político sobre la homofobia, el rechazo hacia la homoparentalidad y el prejuicio hacia las personas con VIH / SIDA. Método . En una muestra de adultos jóvenes de la ciudad de Lima ( N = 279 se evaluaron las variables del estudio a través de instrumentos cuantitativos. Como variables ideológicas se evaluaron los niveles de autoritarismo de ala derecha (RWA, de sus siglas en inglés y la orientación hacia la dominancia social (SDO, de sus siglas en inglés. Resultados . Utilizando diagramas de sendero ( Path Analysis , se observó una correlación directa entre la intolerancia a la ambigüedad, el RWA y la SDO. Se identificó además, una influencia directa de la intolerancia a la ambigüedad, el RWA y la SDO sobre la homofobia y, finalmente, una influencia directa de la homofobia sobre el rechazo hacia la homoparentalidad y el prejuicio hacia las personas con VIH / SIDA. Conclusión . Los individuos más conservadores en lo político son, a la vez, menos tolerantes a la ambigüedad. Por ello, presentan una serie de creencias sobresimplificadas y prejuicios frente a la homosexualidad y a cualquier manifestación sociocultural que consideren asociada a ella.

Identification of a rhodium(iii) complex as a Wee1 inhibitor against TP53-mutated triple-negative breast cancer cells.

Science.gov (United States)

Yang, Guan-Jun; Zhong, Hai-Jing; Ko, Chung-Nga; Wong, Suk-Yu; Vellaisamy, Kasipandi; Ye, Min; Ma, Dik-Lung; Leung, Chung-Hang

2018-03-06

The rhodium(iii) complex 1 was identified as a potent Wee1 inhibitor in vitro and in cellulo. It decreased Wee1 activity and unscheduled mitotic entry, and induced cell damage and death in TP53-mutated triple-negative breast cancer cells. 1 represents a promising scaffold for further development of more potent metal-based Wee1 antagonists.

Influencia del cine en la Generación del 27

Directory of Open Access Journals (Sweden)

Flora Lobato

2011-08-01

Full Text Available Recibido: 19 de marzo, 2008 • Aprobado: 22 de abril, 2008 Este artículo se centra en la influencia que el cine ejerció en la literatura. La aparición del cinematógrafo supuso una revolución en lo cultural; y el mundo de la imagen vino a sumarse a otras artes milenarias, como la pintura y la literatura, las cuales habían servido al hombre para expresar ideas y sentimientos. El cine se considera una rama más de las vanguardias, y, así, el genio creativo de nuestros poetas de la Generación del 27 se vio fructificado, accediendo a territorios antes no explorados por ellos, con lo que sus imágenes y metáforas sobrepasaron los límites conocidos y se adentraron en un universo nuevo, marcado por lógicas de carácter más aperturista.

El cine de Fritz Lang. Influencia de los mitos y arquetipos en su filmografía

OpenAIRE

LÓPEZ HERREROS, ANDRÉS

2013-01-01

El siguiente proyecto presenta un estudio personalizado de la obra cinematográfica del cineasta austríaco Fritz Lang para analizar sus tendencias y vincular los arquetipos a sus películas, principalmente las ideas planteadas por los psicólogos Segmund Freud y Carl Gustav Jung referente a las ideas primarias y secundarias. Para ello se muestra la influencia de la mitología en su narrativa cinematográfica, la presencia de arquetipos y elementos propios del subconsciente colectivo en su cine así...

Estudio descriptivo de la influencia del inglés en la prensa mexicana del siglo XIX

OpenAIRE

Vázquez Amador, María

2015-01-01

L a prensa es reflejo de la realidad vivida en un determinado periodo de tiempo . Este artículo pretende mostrar la influencia de la lengua inglesa en la mexicana a través del análisis de los anglicismos localizados en diversos periódicos publicado s en México durante el siglo XIX . Asimismo se tratará de comprobar la e volución de estas voces a nivel formal y semántico con el fin de determinar si permanecieron y se ada...

Valoración de la influencia de la práctica del fútbol en la evolución de la fuerza, la flexibilidad y la velocidad en población infantil

OpenAIRE

Silvia Sedano Campo*; Gonzalo Cuadrado Sáenz; uan Carlos Redondo Castán

2007-01-01

El objetivo del estudio es valorar la influencia que la práctica regular del fútbol tiene sobre algunos aspectos de la condición física (fuerza explosiva del tren inferior, flexibilidad y velocidad) en niños de entre 7 y 14 años. Por otro lado también pretendemos determinar la correlación existente entre las tres capacidades evaluadas, comparar los resultados con población infantil general y analizar las posibles influencias que la práctica de este deporte puede tener en el desarrollo evoluti...

Influencia de las medidas institucionales y la competencia tecnológica sobre la docencia universitaria a través de plataformas digitales

Directory of Open Access Journals (Sweden)

Tirado, Ramón

2012-06-01

Full Text Available The objective of this study is to empirically test the theoretical model that explains the influence of primary and secondary factors on the integration of digital platforms in university teaching. A sample of 495 teachers from universities in Andalusia completed an online questionnaire that analysed the functions of usage, the digital materials used, the didactic and technological competence of the teaching staff, the support measures adopted by the institutions and the effect on teaching of platform use. Prior factor analysis and the application of the Amos program enabled us to develop a structural equation model to corroborate the indirect influence of the support measures and institutional recognition on teachers in their use of the platforms, and the direct influence of the teachers’ technological proficiency. Este estudio tiene como objetivo poner a prueba empíricamente el modelo teórico que explica la influencia de los factores de primer y segundo orden sobre la integración de las plataformas digitales en la docencia universitaria. Para ello, sobre una muestra de 495 profesores universitarios andaluces, se aplica un cuestionario online que analiza las funciones de uso, materiales digitales utilizados, competencia didáctica y tecnológica del profesorado, medidas de impulso institucionales, y efectos didácticos del uso. El análisis factorial previo y la aplicación del programa Amos permite la elaboración un modelo de ecuación estructural que corrobora la influencia indirecta de las medidas de apoyo y el reconocimiento institucional sobre los efectos didácticos del uso de plataformas, así como la influencia directa de la competencia tecnológica del profesorado.

Cambios en la gestión contable: influencia de las nuevas tecnologías

OpenAIRE

Falcón Pérez, Esther; Fuentes Perdomo, Juana; Serrano Moracho, Francisco

2015-01-01

[ES]En el trabajo que presentamos se revisan los nuevos enfoques de dirección de empresas basados en gestión de procesos y gestión de riesgos, y su influencia en el futuro de la información económica-financiera, lo que dará lugar a cambios acelerados por las más avanzadas aplicaciones informáticas de la contabilidad y las prácticas de auditoría, puesto que en la práctica diaria de las empresas, tanto la auditoría como la información contable y los sistemas de información, contemplan ya dicho ...

Influencia del modelo elástico en la dinámica de estructuras articuladas

Directory of Open Access Journals (Sweden)

Muñoz, M.

1997-06-01

Full Text Available Influence in the dynamics of an articulated structure of sweep wooden, of the approximation to the material tension-deformation properties. A time-domain calculation method by finite differences has been used with a model of lumped masses in the nodes.

Se estudia la influencia en la dinámica de una estructura articulada de barras de madera, de la aproximación a las propiedades tensión-deformación del material. Se ha adoptado un método de cálculo por diferencias finitas en el dominio del tiempo, con un modelo de masas concentradas en los nodos.

Influencia personal de los candidatos y competencia espacial en las elecciones presidenciales venezolanas de 2006 a 2013

Directory of Open Access Journals (Sweden)

Julen Berasaluce Isa

2016-10-01

Full Text Available En la presente investigación se justifica la construcción de un modelo de competencia espacial para las elecciones presidenciales venezolanas con base en el número efectivo de partidos y los índices de polarización ponderada y escalar. El modelo presentado permite construir una clasificación de la influencia personalista de los candidatos, como resto no explicado por una competencia ideológica espacial. Además de clasificar a los candidatos de las tres últimas elecciones presidenciales por su influencia personal, se ofrecen previsiones para futuros comicios tomando como base el cambio de la distribución ideológica. Para la construcción del modelo, se consideran los supuestos de voto ideológico simétrico y una sola dimensión. / This research project presents a spatial competition model for Venezuelan presidential elections based on the effective number of parties and weighted and scalar polarization indices. The model makes it possible to construct a classification of candidates’ influence based on personality as an remainder unexplained by spatial ideological competition. In addition to classifying the candidates in the last three presidential elections by their personal influence, the authors offer predictions for future balloting based on change in ideological distribution. Suppositions of symmetrical ideological votes and a single dimension were used to build the model.

La influencia del género en los estilos de liderazgo y dirección

OpenAIRE

García Ruiz, Luna

2013-01-01

Este Trabajo Fin de Máster (TFM) pretende llevar a cabo un análisis de la influencia del género en los estilos de liderazgo y en los estilos de dirección empleados por los directores/as que ocupan cargos de responsabilidad, a través de la revisión y estudio de diversas publicaciones. En primer lugar, y con el fin de poder llegar a concluir si el género puede ser un factor influyente en los estilos de liderazgo y dirección, hemos tratado de establecer a través de las definiciones aportadas ...

Effects of Induction Heat Bending Process on Microstructure and Corrosion Properties of ASME SA312 Gr.TP304 Stainless Steel Pipes

International Nuclear Information System (INIS)

Kim, Nam In; Kim, Young Sik; Kim, Kyung Soo; Chang, Hyun Young; Park, Heung Bae; Sung, Gi Ho; Sung, Gi Ho

2015-01-01

The usage of bending products recently have increased since many industries such as automobile, aerospace, shipbuilding, and chemical plants need the application of pipings. Bending process is one of the inevitable steps to fabricate the facilities. Induction heat bending is composed of compressive bending process by local heating and cooling. This work focused on the effect of induction heat bending process on the properties of ASME SA312 Gr. TP304 stainless steel pipes. Tests were performed for base metal and bended area including extrados, intrados, crown up, and down parts. Microstructure was analyzed using an optical microscope and SEM. In order to determine intergranular corrosion resistance, Double Loop Electrochemical Potentiokinetic Reactivation (DL-EPR) test and ASTM A262 practice A and C tests were done. Every specimen revealed non-metallic inclusion free under the criteria of 1.5i of the standard and the induction heat bending process did not affect the non-metallic inclusion in the alloys. Also, all the bended specimens had finer grain size than ASTM grain size number 5 corresponding to the grain sizes of the base metal and thus the grain size of the pipe bended by induction heat bending process is acceptable. Hardness of transition start, bend, and transition end areas of ASME SA312 TP304 stainless steel was a little higher than that of base metal. Intergranular corrosion behavior was determined by ASTM A262 practice A and C and DL-EPR test, and respectively step structure, corrosion rate under 0.3 mm/y, and Degree of Sensitization (DOS) of 0.001 - 0.075 % were obtained. That is, the induction heat bending process didn't affect the intergranular corrosion behavior of ASME SA312 TP304 stainless steel

Influencia de los estilos de crianza en el desarrollo de conductas adictivas hacia el consumo de drogas

OpenAIRE

Sarabia Cadaya, Eva María

2015-01-01

Dado que el consumo de drogas supone un importante problema a nivel social, y la prevalencia del mismo en la adolescencia es significativamente elevada, el propósito fundamental de este proyecto ha sido destacar la influencia que ejerce la familia, en general, y los estilos parentales, en particular, sobre los hijos en el desarrollo de conductas adictivas hacia las drogas. Además de hacer referencia a la investigación de Baumrind (1971,1991), pionera en los estudios sobre estilos parent...

Influencia de costos totales en los presupuestos de ventas en MYPEs confeccionistas textiles en Los Olivos 2016

OpenAIRE

Guevara Hermoza, Luis Alberto

2017-01-01

Esta investigación tiene el interés de demostrar la influencia de los costos totales en el presupuesto de ventas de productos terminados en las empresas MYPEs textiles de confecciones. Estas empresas son dirigidas por sus dueños quienes no cuentan o tienen escasa formación para los negocios y, en consecuencia, enfrentan problemas sin herramientas adecuadas para resolverlos. Usualmente cuando el director de la MYPE se encuentra con poco efectivo o sus ganancias son muy reduci...

Influencia de Las Cargas Térmicas Sobre Flujos de Aire Generados en Una Habitacíon

DEFF Research Database (Denmark)

Olmedo, Inés; Ruiz de Adana Santiago, Manuel; Nielsen, Peter V.

2011-01-01

El flujo de aire en el interior de un local generado con un sistema de ventilación y difusión de aire puede verse afectado por las cargas térmicas internas del local. En este trabajo, se estudia la influencia de las cargas térmicas internas debidas a personas en el flujo de aire interior de un lo...... difusor textil de baja velocidad. Los resultados obtenidos pueden resultar de interés para validar las predicciones obtenidas a partir de modelos numéricos de difusores....

Influencia de los procesos de depuración sobre la toxicidad de las aguas residuales textiles.

OpenAIRE

Crespi Rosell, Martí; Vilaseca i Vallvé, Ma. Mercè (Maria Mercè)

1983-01-01

La toxicidad de algunos productos utilizados en los procesos de tintura y acabado de los artículos textiles es muy elevada. En este trabajo se determina la toxicidad frente a la Dafnia Magna de algunos productos químicos utilizados en la Industria Textil. También se estudia la influencia de los procesos de depuración sobre la toxicidad de las aguas residuales textiles. La toxicité de quelques produits utilisés dans les processus de teinture et d'ennoblissement des articles textiles est ...

Influencia de la lactancia materna en la prevención de hábitos bucales deformantes

OpenAIRE

Fuguet Boullon, Julia R; Betancourt García, Ana Ibis; Ochoa Jiménez, Lourdes; González Pérez, Marlén; Crespo García, Arelis; Viera Rodríguez, Dianeya

2014-01-01

Introducción: la lactancia materna constituye uno de los pilares fundamentales de la promoción de salud y la prevención de numerosas enfermedades. Objetivo: identificar la influencia de la lactancia materna exclusiva con la presencia de hábitos bucales deformantes. Métodos: se realizó un estudio observacional descriptivo de corte transversal en niños 3-5 años de ambos sexos, pertenecientes al círculo infantil “20 primaveras”, del municipio de Cienfuegos, en el período comprendido entre septie...

In vitro antioxidant and cytoprotective properties of Maillard reaction products from phloridzin-amino acid model systems.

Science.gov (United States)

Han, Linna; Li, Feng; Yu, Qijian; Li, Dapeng

2018-01-01

The aim of this study was to investigate in vitro antioxidant activities and cytoprotective effect of Maillard reaction products (MRPs) from phloridzin (Pz)-amino acid model systems. Their structures were also characterised by Fourier transform-infrared spectroscopy (FTIR). MRPs were prepared from the Pz-methionine (Met), Pz-lysine (Lys), Pz-isoleucine (Ile), Pz-histidine (His) or Pz-glutamic acid (Glu) model system. The Pz-Lys MRPs, rich in antioxidant potency, were subjected to ultrafiltration to yield four MRPs fractions with different molecular weights (Mw). The fraction with Mw 30-50 kDa had significantly (P Maillard reaction. The results obtained in this study may provide some basis for the purported health-promoting effects of MRPs and their potential application as antioxidant agents in food industry. Also, it is important for our understanding of the variation of bioactive substances in food during thermal processing. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

Abordajes teóricos en la comprensión de la influencia de los recursos económicos en la actividad física

OpenAIRE

Montenegro Mendivelso, Mónica

2015-01-01

Estudio cualitativo que analiza los abordajes teóricos utilizados por diferentes autores en la comprensión de la influencia de los recursos económicos en la actividad física desde los modelos de determinantes y determinación social.

La Influencia de la Temperatura Exterior sobre los Sistemas de Ventilación Natural en Grandes Atrios

Directory of Open Access Journals (Sweden)

Espina, P.

2008-09-01

Full Text Available Natural venting systems present numerous advantages opposite to the mechanical exhaust for the smoke control – a reduction in both facilities and maintenance costs–. Nevertheless, the influence of numerous factors affects significantly their efficacy: the architectural characteristics of the building, the direction and wind velocity, the proximity of tall buildings, the smoke temperature, the environmental interior and exterior temperatures, the existence of snow or ice on the ceiling, etc. All of them are important, but the influence of the environmental exterior temperature plays a decisive role. The goal of this Investigation Research was to evaluate the influence of the external temperature on the smoke movement and the hot layer descent regarding the efficacy of the natural venting systems installed for the smoke control in large atria. The analysis was developed using the ‘Fire Dynamics Simulator - FDS’ model (1, a computational fluid dynamics (CFD model of fi re-driven fluid flow for the study of fire. The results demonstrated that a design that does not contemplate this factor can turn out to be inadequate, since it has a decisive influence to guarantee human safety. The obtained results showed very significant differences about the different parameters linked to the smoke movement in an atrium.Entre las diferentes estrategias de Control y Evacuación de los Humos del Incendio, los sistemas de ventilación natural presentan numerosas ventajas frente a la ventilación mecánica – fundamentalmente una menor inversión tanto en equipos como en gastos de mantenimiento –. Sin embargo, su eficacia depende de la influencia ejercida por numerosos factores – características arquitectónicas del edificio, dirección y velocidad del viento, proximidad de edificios de gran altura, temperatura de los humos, temperatura ambiental interior, temperatura en el exterior del recinto, existencia de nieve o hielo en

Microstructural investigation of the oxide formed on TP 347H FG during long-term steam oxidation

DEFF Research Database (Denmark)

Hansson, Anette Nørgaard; Danielsen, Hilmar Kjartansson; Grumsen, Flemming Bjerg

2010-01-01

The long-term oxidation behaviour of TP347H FG in ultra supercritical steam conditions was assessed by exposing the steel in test superheater loops in a Danish coal-fired power plant and characterising the oxide layer with reflective light and electron microscopy. Double layered oxide scales formed...... during steam oxidation. TEM investigations reveal that the inner oxide layer consists of particles of metallic Ni/Fe and Fe-Cr spinel in the interior of the former alloy grains and a compact layer of Fe-Cr spinel and Cr2O3 along the former alloy grain boundaries. The morphology suggests that the inner...

Tetra-2,3-pyrazinoporphyrazines with externally appended pyridine rings. 15. Effects of the pyridyl substituents and fused exocyclic rings on the UV-visible spectroscopic properties of Mg(II)-porphyrazines: a combined experimental and DFT/TDDFT study.

Science.gov (United States)

Donzello, Maria Pia; De Mori, Giorgia; Viola, Elisa; Ercolani, Claudio; Ricciardi, Giampaolo; Rosa, Angela

2014-08-04

Two new Mg(II) porphyrazine macrocycles, the octakis(2-pyridyl)porphyrazinato-magnesium(II), [Py8PzMg(H2O)], and the tetrakis-[6,7-di(2-pyridyl)quinoxalino]porphyrazinato-magnesium(II), [Py8QxPzMg(H2O)], were prepared by Mg-template macrocyclization processes, and their general physicochemical properties were examined. The previously reported porphyrazine analog, the tetrakis-2,3-[5,6-di(2-pyridyl)-pyrazino]porphyrazinato-magnesium(II), [Py8PyzPzMg(H2O)], has been also considered in the present work. The UV-visible solution spectra in nonaqueous solvents of this triad of externally octapyridinated Mg(II) complexes exhibit the usual profile observed for phthalocyanine and porphyrazine macrocycles, with intense absorptions in the Soret (300-450 nm) and Q band (600-800 nm) regions. It is observed that the Q band maximum sensibly shifts toward the red with peak values at 635 → 658 → 759 nm along the series [Py8PzMg(H2O)], [Py8PyzPzMg(H2O)], and [Py8QxPzMg(H2O)], as the extension of the macrocycle π-system increases. TDDFT calculations of the electronic absorption spectra were performed for the related water-free model compounds [Py8PzMg], [Py8PyzPzMg], and [Py8QxPzMg] to provide an interpretation of the UV-visible spectral changes occurring upon introduction of the pyrazine and quinoxaline rings at the periphery of the Pz macrocycle. To discriminate the electronic effects of the fused exocyclic rings from those of the appended 2-pyridyl rings, the UV-visible spectra of [PzMg] and [PyzPzMg] were also theoretically investigated. The theoretical results prove to agree very well with the experimental data, providing an accurate description of the UV-visible spectra. The observed spectral changes are interpreted on the basis of the electronic structure changes occurring along the series.

Fracture toughness evaluations of TP304 stainless steel pipes

International Nuclear Information System (INIS)

Rudland, D.L.; Brust, F.W.; Wilkowski, G.M.

1997-02-01

In the IPIRG-1 program, the J-R curve calculated for a 16-inch nominal diameter, Schedule 100 TP304 stainless steel (DP2-A8) surface-cracked pipe experiment (Experiment 1.3-3) was considerably lower than the quasi-static, monotonic J-R curve calculated from a C(T) specimen (A8-12a). The results from several related investigations conducted to determine the cause of the observed toughness difference are: (1) chemical analyses on sections of Pipe DP2-A8 from several surface-cracked pipe and material property specimen fracture surfaces indicate that there are two distinct heats of material within Pipe DP2-A8 that differ in chemical composition; (2) SEN(T) specimen experimental results indicate that the toughness of a surface-cracked specimen is highly dependent on the depth of the initial crack, in addition, the J-R curves from the SEN(T) specimens closely match the J-R curve from the surface-cracked pipe experiment; (3) C(T) experimental results suggest that there is a large difference in the quasi-static, monotonic toughness between the two heats of DP2-A8, as well as a toughness degradation in the lower toughness heat of material (DP2-A8II) when loaded with a dynamic, cyclic (R = -0.3) loading history

120. Influencia del desajuste paciente-prótesis en la morbimortalidad intrahospitalaria del paciente joven intervenido de recambio valvular aórtico por estenosis grave

Directory of Open Access Journals (Sweden)

D. Hernández-Vaquero

2012-04-01

Conclusiones: El desajuste paciente-prótesis es un hallaz-go frecuente en el paciente joven intervenido de recambio valvular aórtico por estenosis grave. Además, su influencia a los 30 días de seguimiento parece relevante.

Inteligencia emocional y su influencia en el clima organizacional en los niveles gerenciales medios de las PYMES

OpenAIRE

Cira de Pelekais; Angel Nava; Luis Tirado

2006-01-01

El propósito fue determinar la influencia de la Inteligencia Emocional sobre Clima Organizacional en los niveles gerenciales medios de las PYMES. La investigación fue caracterizada bajo un enfoque descriptivo explicativo, con un diseño no experimental transeccional. Como unidades de análisis fueron tomadas las PYMES adscritas a la cámara de Industria y Comercio de Cabimas, totalizando 100 empresas activas. Para la recolección de la información se utilizó un cuestionario autoadministrado, con ...

Influencia de la solidez en un aerogenerador VAWT con rotor H-Darrieus y perfiles simétricos

OpenAIRE

Alonso Gómez, Álvaro

2016-01-01

Mediante el empleo de la Mecánica Computacional de Fluidos, se estudia la influencia que tiene el grado de solidez en el comportamiento de un aerogenerador. Se estudia un aerogenerador de eje vertical con rotor H-Darrieus y tres perfiles simétricos en el que se analizan tres grados de solidez diferentes, generándose por lo tanto tres modelos, para cada uno de los cuales se obtiene la curva de rendimiento correspondiente. Para construir la curva se van realizando simulaciones a diferentes ve...

Influencia de las Interacciones Sociales en el Rendimiento Académico. Aproximación Bibliográfica

OpenAIRE

García-Séiquer, Mercedes

2013-01-01

En el presente trabajo se ha realizado un estudio sobre la influencia de los procesos de socialización y las interacciones sociales sobre el rendimiento académico. Se ofrece una amplia visión de diferentes estudios que muestran cómo la presencia de habilidades sociales exitosas beneficia las relaciones personales, fomentan el bienestar emocional, el autoconcepto, las expectativas de logro, el clima social y el rendimiento en general de la persona. Este trabajo plasma cómo la disposición de un...

An activator of transcription regulates phage TP901-1 late gene expression

DEFF Research Database (Denmark)

Brøndsted, Lone; Pedersen, Margit; Hammer, Karin

2001-01-01

bp contains both the promoter and the region necessary for activation by ORF29. The transcriptional start site of the promoter was identified by primer extension to position 13073 on the TP901-1 genome, thus located 87 bp downstream of orf29 in a 580-bp intergenic region between orf29 and orf30....... Furthermore, the region located -85 to -61 bp upstream of the start site was shown to be necessary for promoter activity. During infection, the transcript arising from the late promoter is fully induced at 40 min postinfection, and our results suggest that a certain level of ORF29 must he reached in order...... to activate transcription of the promoter. Several lactococcal bacteriophages encode ORF29 homologous proteins, indicating that late transcription may be controlled by a similar mechanism in these phages. With the identification of this novel regulator, our results suggest that within the P335 group...

Migración e influencia urbana en el consumo de alimentos en dos comunidades Mayas de Yucatán

Directory of Open Access Journals (Sweden)

María Guadalupe Gutiérrez Carbaja

2017-01-01

Full Text Available Objetivo. Analizar los principales efectos que ejercen la migración y la influencia urbana sobre las formas de adquisición y consumo de los productos alimenticios en dos comunidades mayas de Yucatán. Metodología. Se basó en una encuesta por muestreo estadístico a jefas de familias. El procedimiento fue por conglomerados. Resultados. Se encontró que el porcentaje y motivos de la migración fueron diferentes; el tipo de alimento en la dieta y su forma de adquisición dependen de la disponibilidad, el acceso físico y de la economía de las familias. Además los sistemas de producción milpa y traspatio influyeron sobre la composición de la dieta habitual. Limitaciones. La principal limitación es la falta de capacidad de los sistemas agrícolas tradicionales para satisfacer las necesidades alimenticias, lo cual se profundiza por el abandono paulatino de la producción primaria. Conclusión. La migración, asociada a la influencia urbana, modificó la dinámica familiar y la producción de autoconsumo, aspectos que influyeron sobre la adquisición de los alimentos y diversidad de la dieta familiar.

Wee1 Kinase Inhibitor AZD1775 Radiosensitizes Hepatocellular Carcinoma Regardless of TP53 Mutational Status Through Induction of Replication Stress

Energy Technology Data Exchange (ETDEWEB)

Cuneo, Kyle C., E-mail: kcuneo@umich.edu; Morgan, Meredith A.; Davis, Mary A.; Parcels, Leslie A.; Parcels, Joshua; Karnak, David; Ryan, Caila; Liu, Na; Maybaum, Jonathan; Lawrence, Theodore S.

2016-06-01

Purpose: Wee1 kinase inhibitors are effective radiosensitizers in cells lacking a G{sub 1} checkpoint. In this study we examined the potential effect of Wee1 kinase inhibition on inducing replication stress in hepatocellular carcinoma (HCC). Methods and Materials: Five independent datasets from the Oncomine database comparing gene expression in HCC compared to normal tissue were combined and specific markers associated with Wee1 sensitivity were analyzed. We then performed a series of in vitro experiments to study the effect of Wee1 inhibition on irradiated HCC cell lines with varying p53 mutational status. Clonogenic survival assays and flow cytometry using anti-γH2AX and phospho-histone H3 antibodies with propidium iodide were performed to study the effect of AZD1775 on survival, cell cycle, and DNA repair. Additionally, nucleoside enriched medium was used to examine the effect of altering nucleotide pools on Wee1 targeted radiation sensitization. Results: Our analysis of the Oncomine database found high levels of CDK1 and other cell cycle regulators indicative of Wee1 sensitivity in HCC. In our in vitro experiments, treatment with AZD1775 radiosensitized and chemosensitized Hep3B, Huh7, and HepG2 cell lines and was associated with delayed resolution of γH2AX foci and the induction of pan-nuclear γH2AX staining. Wee1 inhibition attenuated radiation-induced G{sub 2} arrest in the Hep3B (TP53 null) and Huh7 (TP53 mutant) cell lines but not in the TP53 wild-type cell line HepG2. Supplementation with nucleosides reversed the radiation-sensitizing effect of AZD1775 and reduced the amount of cells with pan-nuclear γH2AX staining after radiation. Conclusions: Radiation sensitization with Wee1 inhibition occurs in cells regardless of their p53 mutational status. In this study we show for the first time that replication stress via the overconsumption of nucleotides plays an important role in AZD1775-induced radiation sensitization.

El factor internacional en la transición española: la influencia del contexto internacional y el papel de las potencias centrales

Directory of Open Access Journals (Sweden)

Juan Carlos PEREIRA CASTAÑARES

2010-02-01

Full Text Available RESUMEN: La transición española hacia la democracia ha sido objeto de numerosos estudios; sin embargo, en la mayoría de los casos la transición se ha analizado desde el punto de vista interno o de los protagonistas nacionales. En nuestra investigación analizamos, por el contrario, la influencia del factor internacional en ese proceso de transición. Entendemos el factor internacional desde dos perspectivas: la incidencia de este factor hacia el interior, es decir, la influencia del contexto internacional y sus actores; por otro lado, hacia el exterior, es decir, cómo se produce también una transición en el proceso de formulación, decisión y ejecución de la política exterior del autoritarismo a la democracia. Se presenta en este trabajo unos primeros resultados de la investigación sobre la influencia del contexto internacional, planteando una propuesta teórico-metodológica que se aplica al caso español. Por último, nos centramos más concretamente en la intervención de Francia, Alemania y Estados Unidos, como potencias centrales en esa influencia internacional. Palabras clave: Transición Española, Contexto Internacional, Francia, Alemania, Estados Unidos ABSTRACT: The Spanish Transition to democracy has been the aim of many studies, but the majority of the cases the transition has been analysed from the inside point of view or from the perspective of national actors. In our reserach we study, on the other hand, the International Factor in this process of transition. We consider the International Factor from two perspectives: on the one hand, the impact of this factor toward the domestic affairs, the influence of International Context and its actors; and, on the other hand, toward outside, the way of transition in the process of formulation, decision and execution of the foreign policy from authoritarianism to democracy. We show in this work a first results of the research about the influence of the International Context and

Influencia sobre parámetros biológicos de Tagosodes orizicolus relacionados con el Virus Hoja Blanca y su permanencia en invernaderos.

Directory of Open Access Journals (Sweden)

Rafael Meneses

2007-03-01

Full Text Available Con el objetivo de determinar la influencia que ejerce el Virus Hoja Blanca (VHB sobre el insecto, se estudiaron tres colonias de T. orizicolus, procedentes de diferentes zonas arroceras de Colombia (Tolima, Costa y Jamundí En las colonias de Costa (3 años y Tolima, (9 meses, de multiplicación en condiciones semicontroladas de invernadero, no se encontró influencia negativa del virus en la longevidad de machos y hembras, y la fecundidad diaria, no ocurriendo de modo semejante en los insectos de Jamundí (4 meses que si se manifestaron efectos deletéreos en todos los parámetros antes señalados; aspecto éste muy importante en la interacción entre entomólogos, virólogos y mejoradores, en la calidad de las evaluaciones de resistencia a esta plaga y al VHB de las líneas de arroz, depende el grado de resistencia o susceptibilidad de la nuevas variedades comerciales y éste es el principal factor en el Manejo Integrado de T. orizicolus y del VHB.

Investigating heat and temperature regime of the combustion chamber furnace screen of the TP 100A steam generator in the Varna thermal power plant

Energy Technology Data Exchange (ETDEWEB)

Mikhlevski, A; Buchinski, B; Dashkiev, Yu; Radzievski, V; Petkov, Kh [Kievski Politekhnicheski Institut (USSR)

1988-01-01

In the course of 10 year operation of six TP 100A steam generators 72 emergency operation interruptions occurred due to the piercing of screen pipes in the combustion chamber. According to investigations carried out by the NPO, CKT, VTI, KPI and Soyuzenergo institutes, the damage occurred mainly because of the destructive influence of external gas corrosion processes, overheating and fatigue of metallic pipes, as well as unstable heat and temperature regime in the combustion chamber. Large-scale measurements of the main thermodynamic parameters of the combustion chamber of the TP-100A steam generator were carried out in order to increase service life of screen pipes. It was found that the temperature of screen pipes increases 2.5 C/month because of deposition of sediments. Regular cleaning of screen pipes in intervals of 18 months is recommended as a very efficient means of prolonging their service life. 1 ref.

Geología de la zona de influencia de la falla otú en el distrito minero segovia-remedios

Directory of Open Access Journals (Sweden)

MILTON ÁLVAREZ GALINDEZ

2007-01-01

Full Text Available Se presenta la caracterización geológica y estructural de la zona de influencia de la falla Otú en jurisdicción del distrito minero Segovia-Remedios. Además, se proponen controles mecánicos para las mineralizaciones auríferas, que si bien son compatibles, en parte, con un modelo de depósitos de oro orogénico, dejan abiertas otras posibles interpretaciones

La influencia de factores sistémicos en el uso de las prótesis totales Influence of systemic factors in use of total prostheses

Directory of Open Access Journals (Sweden)

Carolina de Andrade Lima Chaves

2009-03-01

Full Text Available Considerando la posible influencia de las enfermedades sistémicas y de los medicamentos utilizados para su tratamiento en la cavidad bucal, el presente estudio se propone realizar una revisión de la literatura sobre el tema, enfatizando la influencia de estos factores en el uso de las prótesis totales (PTs, con el fin de proporcionar al cirujano-dentista un mejor conocimiento científico para la realización de ese tipo de tratamiento rehabilitador.According to the possible influence of systemic diseases and of drugs used for its treatment in buccal cavity, the aim of present study is to carry out a review of literature on this topic, emphasizing on influence of these factors on use of the total prostheses (PTs, allowing to surgeon-dentist a better scientific knowledge to creation of this type of rehabilitation treatment.

La otra América. Influencia de la literatura estadounidense en Roberto Bolaño

OpenAIRE

Fernández Díaz, José Javier

2014-01-01

Los vínculos entre la literatura estadounidense y la obra de Roberto Bolaño han sido objeto de numerosas reseñas y artículos. Sin embargo su estudio hasta la fecha no ha cristalizado en un trabajo monográfico. «La otra América: influencias de la literatura estadounidense en Roberto Bolaño» tiene entre sus objetivos construir un mapa literario que dé cuenta del diálogo entre textos y autores. El corpus de la tesis se ha extraído de las más de cien menciones explícitas a escritores y libros est...

Trastornos de la personalidad: influencia sobre la conducta delictiva y repercusiones forenses en la jurisdicción penal

OpenAIRE

González Guerrero, Laura

2011-01-01

Los trastornos de la personalidad representan exacerbaciones desadaptativas e inflexibles de los rasgos individuales, que tienen su reflejo en la cognición, emoción, motivación, relaciones y conductas de los individuos que los padecen. El principal objetivo del estudio desarrollado ha sido alcanzar un mayor conocimiento sobre cada uno de los trastornos específicos de la personalidad en el contexto jurídico penal, especialmente su influencia diferencial sobre la conducta delictiva. Para ello s...

Influencia de la composici??n del medio de cultivo sobre la producci??n de pigmento por Streptococcus agalactiae

OpenAIRE

Carazo Carazo, Concepci??n

1995-01-01

Se ha estudiado la influencia de la composici??n del medio de cultivo sobre la producci??n de pigmento por s. agalactiae partiendo de un medio de cultivo dise??ado para tal finalidad (new granada medium), eliminando uno a uno los componentes incluidos en su formulaci??n original. Almid??n, proteosa peptona (difco), suero, fosfatos y metrotexato influyen de forma importante en la formaci??n y/o visualizaci??n del pigmento producido por este microorganismo. Se ha determinado que la fracci??n de...

Influencia de los Factores en la Aceptación de la Vacunación contra el Virus Papiloma Humano en Padres de Familia de Escolares de las I.E. Secundarias de Juliaca 2014

OpenAIRE

Beltrán Beltrán, Rosita Graciela

2016-01-01

La investigación efectuada trata específicamente sobre la influencia de los factores en la aceptación de la vacunación contra el virus papiloma humano en padres de familia de escolares de las I.E. secundarias de la ciudad de Juliaca durante el año 2014. El propósito del trabajo de investigación ha sido determinar la medida en que el nivel de influencia de los factores en la aceptación de la vacunación contra el virus papiloma humano en padres de familia de escolares de las I.E. secund...

Synthesis of total protein (TP) and myosin heavy chain (HC) isozymes in pressure overloaded rabbit hearts

International Nuclear Information System (INIS)

Nagai, R.; Martin, B.J.; Pritzl, N.; Zak, R.; Low, R.B.; Stirewalt, W.S.; Alpert, N.R.; Litten, R.Z.

1986-01-01

Pulmonary artery banding (PO) leads to a rapid increase in right ventricular (RV) weight as well as a shift toward β myosin isozyme. They determined: (1) the contributions of changes in the capacity (RNA content) and efficiency of total protein synthesis to the increase in RV weight; and (2) the relative contributions of translational and pretranslational mechanisms to the shift in myosin HC isotypes. The rates of synthesis in vivo of TP, α- and β-HC were measured by a constant infusion technique using 3 H-leucine. TP synthesis was 7 +/- 2(SD) mg/day in control (RV:367 +/- 70 mg) and was increased by 2.6 fold at day 2 and 2.9 fold at day 4 following PO (p < 0.01). RV RNA content was increased by 83% at day 2 and 103% at day 4 PO (p < 0.05). The efficiency of synthesis (rate/RNA) was also significantly higher at these time points (1.4- and 1.3-fold). β-HC synthesis was 0.6 +/- 0.2 mg/day in control and increased by 2.6 fold at day 2 and 3.5 fold at day 4 following PO. In contrast, the rate of synthesis of α-HC was unchanged. The relative rates of β-HC to total HC synthesis was correlated linearly with the relative levels of β-myosin mRNA as measured by S1 nuclease mapping. They conclude that increases in the proportion of β-HC myosin following PO is due to increases in the relative amount of β-myosin mRNA and therefore involves modulation of a pretranslational mechanism

Influencia de algunas variables en la biosorción de plomo con residuos agrícolas

OpenAIRE

Hernáinz Bermúdez de Castro, Francisco; Blázquez García, Gabriel; Calero de Hoces, Mónica; Martín-Lara, Mª Ángeles

2008-01-01

En este trabajo se ha estudiado la biosorción de plomo presente en medios acuosos utilizando como sólidos sorbentes hueso de aceituna, alpeorujo y ramón del olivo, analizando la influencia sobre el proceso del pH, concentración de biosorbente, tamaño de partícula y tiempo de contacto. Los resultados muestran que en medios muy ácidos no se produce retirada de plomo con ninguno de los sólidos, aumentando el porcentaje de plomoretirado a medida que se eleva el pH del medio hastaalcanzar un valor...

INFLUENCIA DE LA MADERA JUVENIL DE PINO RADIATA SOBRE LAS PROPIEDADES MECÁNICAS DE TABLEROS OSB

OpenAIRE

PECHO,Robert; ANANIAS,Rubén A; BALLERINI,Aldo; CLOUTIER,Alain

2004-01-01

En este trabajo se estudia la influencia de la madera juvenil de pino radiata (Pinus radiata D. Don) sobre las propiedades mecánicas de tableros de hojuelas orientadas OSB. La madera para los ensayos es recogida de 10 árboles en pié de 26 años creciendo en plantaciones manejadas de la Octava región, Chile. La determinación de madera juvenil se realiza observando la variación radial de los anillos de crecimiento de la madera, usando un analizador de anillos en base a rayos X. Los detalles de l...

El delito de tráfico de influencias en la jurisprudencia de la Corte Suprema de Justicia

OpenAIRE

Piedrahita Jaramillo, Renán

2012-01-01

El presente ensayo está dirigido al estudio desde la jurisprudencia del delito de tráfico de influencias (arts. 411 y 411 A). La justificación del estudio propuesto está dada por la necesidad de mirar la parte especial del Código Penal, en tanto que la mayoría de la doctrina nacional se ha interesado por el estudio de su parte general, recabando en los institutos que conforman el injusto penal y el tipo subjetivo, entre otros temas, sin mirar la necesidad de sentar las bases de interpretación...

Tiempo y venta: la influencia del tiempo de espera en la satisfacción con el servicio

OpenAIRE

Gavilán Bouzas, Diana

2012-01-01

Trabajo de investigación sobre la influencia de las esperas que tienen lugar antes, durante y/o al finalizar los proceso de demanda de bienes o servicios. Partiendo de la hipótesis teórica de que el efecto perjudicial de la espera solo se produce cuando la intensidad de la pérdida excede la ganancia de utilidad que puede reportar la espera, se establecen tres efectos posibles de la espera: satisfacción, indiferencia, e insatisfacción; de los cuales, los dos primeros podrían asimilarse a la ca...

Influencia del evento el niño-oscilacion del sur sobre las precipitaciones en cinco ciudades del litoral ecuatoriano

OpenAIRE

Espol; Rodriguez Aguilar, Jose Antonio; Luna Rodriguez, Javier Alejandro

2017-01-01

La investigación se realizó con el objetivo de evaluar la influencia del fenómeno enos, caracterizado por los índices oni e icen, sobre las precipitaciones ocurridas en el período de 1975-2016 en ciudades del litoral ecuatoriano, con la finalidad de que contribuya al diseño de lineamientos para mejorar la respuesta de la población ante la posible presencia del evento Guayaquil MAGISTER EN CAMBIO CLIMATICO

A Novel Interaction of Ecdysoneless (ECD) Protein with R2TP Complex Component RUVBL1 Is Required for the Functional Role of ECD in Cell Cycle Progression.

Science.gov (United States)

Mir, Riyaz A; Bele, Aditya; Mirza, Sameer; Srivastava, Shashank; Olou, Appolinaire A; Ammons, Shalis A; Kim, Jun Hyun; Gurumurthy, Channabasavaiah B; Qiu, Fang; Band, Hamid; Band, Vimla

2015-12-28

Ecdysoneless (ECD) is an evolutionarily conserved protein whose germ line deletion is embryonic lethal. Deletion of Ecd in cells causes cell cycle arrest, which is rescued by exogenous ECD, demonstrating a requirement of ECD for normal mammalian cell cycle progression. However, the exact mechanism by which ECD regulates cell cycle is unknown. Here, we demonstrate that ECD protein levels and subcellular localization are invariant during cell cycle progression, suggesting a potential role of posttranslational modifications or protein-protein interactions. Since phosphorylated ECD was recently shown to interact with the PIH1D1 adaptor component of the R2TP cochaperone complex, we examined the requirement of ECD phosphorylation in cell cycle progression. Notably, phosphorylation-deficient ECD mutants that failed to bind to PIH1D1 in vitro fully retained the ability to interact with the R2TP complex and yet exhibited a reduced ability to rescue Ecd-deficient cells from cell cycle arrest. Biochemical analyses demonstrated an additional phosphorylation-independent interaction of ECD with the RUVBL1 component of the R2TP complex, and this interaction is essential for ECD's cell cycle progression function. These studies demonstrate that interaction of ECD with RUVBL1, and its CK2-mediated phosphorylation, independent of its interaction with PIH1D1, are important for its cell cycle regulatory function. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

TP1 - A computer program for the calculation of reactivity and kinetic parameters by one-dimensional neutron transport perturbation theory

International Nuclear Information System (INIS)

Kobayashi, K.

1979-03-01

TP1, a FORTRAN-IV program based on transport theory, has been developed to determine reactivity effects and kinetic parameters such as effective delayed neutron fractions and mean generation time by applying the usual perturbation formalism for one-dimensional geometry. Direct and adjoint angular dependent neutron fluxes are read from an interface file prepared by using the one-dimensional Ssub(n)-code DTK which provides options for slab, cylindrical and spherical geometry. Multigroup cross sections which are equivalent to those of the DTK-calculations are supplied in the SIGM-block which is also read from an interface file. This block which is usually produced by the code GRUCAL should contain the necessary delayed neutron data, which can be added to the original SIGMN-block by using the code SIGMUT. Two perturbation options are included in TP1: a) the usual first oder perturbation theory can be applied to determine probe reactivities, b) assuming that there are available direct fluxes for the unperturbed reactor system and adjoint fluxes for the perturbed system, the exact reactivity effect induced by the perturbation can be determined by an exact perturbation calculation. According to the input specifications, the output lists the reactivity contributions for each neutron reaction process in the desired detailed spatial and energy group resolution. (orig./RW) [de

The removal of toxic metals from liquid effluents by ion exchange resins. Part II: cadmium(II/ sulphate/Lewatit TP260

Directory of Open Access Journals (Sweden)

Alguacil, F. J.

2002-10-01

Full Text Available The adsorption of cadmium (II, from aqueous sulphate solutions, on Lewatit TP260 resin has been investigated in batch equilibrium experiments. The influence of pH and temperature on metal adsorption capacity have also been examined. The kinetic performance of the resin has been assesed and the results have been correlated by the pore diffusion model. The resin has been used in mini-columns to study its performance under dynamics conditions. The desorption of metal ion is achieved using sulphuric acid (0.25M and 0.5M.

Se estudia la adsorción de cadmio(II, de disoluciones en medio sulfato, sobre la resina Lewatit TP260. La adsorción del metal se ha investigado en función del pH, la temperatura y el tiempo de contacto con la resina. Los estudios cinéticos permiten correlacionar el proceso de intercambio iónico con el modelo de difusión en poro. Se ha empleado el sistema en mini columnas para evaluar el comportamiento de la resina bajo condiciones dinámicas. La desorción del metal se lleva a cabo con disoluciones de ácido sulfúrico (0,25M y 0,5M.

Grate Pallet 8232 (GP-8232) Vehicle Pallet, Evaluation Tests MIL-STD-1660, "Design Criteria for Ammunition Unit Loads" and TP-94-01 (REV 1), "Transportability Testing Procedures"

National Research Council Canada - National Science Library

Barickman, Philip

2003-01-01

.... The testing was conducted for informational purposes only. The GP-8232 Vehicle Pallet was evaluated by the testing procedures set forth in MIL-STD-1660 and TP-94-01 (Rev. 1) testing procedures...

Influencia del programa preventivo promocional en salud escolar y el desarrollo de capacidades en higiene personal en estudiantes

OpenAIRE

Salvador Esquivel, Nilda Elizabeth

2015-01-01

Objetivo: Determinar la influencia del programa preventivo promocional en salud escolar en el desarrollo de capacidades en higiene personal en estudiantes del primer grado de educación primaria de la Institución Educativa N°1211 José María Arguedas, Santa Anita, 2013. Material y métodos: Estudio cuantitativo, de tipo cuasi experimental, de corte transversal, prospectivo. La población fue de 56 estudiantes del 1er. grado A (grupo experimental) y B (grupo control). El estudio tuvo una duraci...

Electrochemical capacitors based on nitrogen-enriched cobalt (II) phthalocyanine/multi-walled carbon nanotube nanocomposites

CSIR Research Space (South Africa)

Lekitima, JN

2013-11-01

Full Text Available nanotubes (MWCNTaf and MWCNTPhNH2) have been studied for application as an electrochemical capacitor in H2SO4. Both MWCNTaf/CoTPyzPz and MWCNTPhNH2/CoTPyzPz gave superior specific capacitance of 1984 and 2028 F.g-1 respectively . This high capacitance... electrodes in (i) 1.0 M H2SO4 electrolyte and (ii) 1.0M Na2SO4 at a scan rate of 10mVs-1. Figure 3 and 4 show the charge-discharge profiles of all the materials. The curves for CoTPyzPz, MWCNTPhNH2/CoTPyzPz and MWCNTaf/CoTPyzPz showed that potential...

Towards Prognostic Profiling of Non-Small Cell Lung Cancer: New Perspectives on the Relevance of Polo-Like Kinase 1 Expression, the TP53 Mutation Status and Hypoxia

Science.gov (United States)

Van den Bossche, Jolien; Deben, Christophe; Op de Beeck, Ken; Deschoolmeester, Vanessa; Hermans, Christophe; De Pauw, Ines; Jacobs, Julie; Van Schil, Paul; Vermorken, Jan Baptist; Pauwels, Patrick; Peeters, Marc; Lardon, Filip; Wouters, An

2017-01-01

Background: Currently, prognosis of non-small cell lung cancer (NSCLC) patients is based on clinicopathological factors, including TNM stage. However, there are considerable differences in patient outcome within a similar staging group, even when patients received identical treatments. In order to improve prognostic predictions and to guide treatment options, additional parameters influencing outcome are required. Polo-like kinase 1 (Plk1), a master regulator of mitotic cell division and the DNA damage response, is considered as a new potential biomarker in this research area. While several studies reported Plk1 overexpression in a broad range of human malignancies, inconsistent results were published regarding the clinical significance hereof. A prognostic panel, consisting of Plk1 and additional biomarkers that are related to the Plk1 pathway, might further improve prediction of patient prognosis. Methods: In this study, we evaluated for the first time the prognostic value of Plk1 mRNA and protein expression in combination with the TP53 mutation status (next generation sequencing), induction of apoptotic cell death (immunohistochemistry for cleaved caspase 3) and hypoxia (immunohistochemistry for carbonic anhydrase IX (CA IX)) in 98 NSCLC adenocarcinoma patients. Results: Both Plk1 mRNA and protein expression and CA IX protein levels were upregulated in the majority of tumor samples. Plk1 mRNA and protein expression levels were higher in TP53 mutant samples, suggesting that Plk1 overexpression is, at least partially, the result of loss of functional p53 (<0.05). Interestingly, the outcome of patients with both Plk1 mRNA and CA IX protein overexpression, who also harbored a TP53 mutation, was much worse than that of patients with aberrant expression of only one of the three markers (p=0.001). Conclusion: The combined evaluation of Plk1 mRNA expression, CA IX protein expression and TP53 mutations shows promise as a prognostic panel in NSCLC patients. Moreover

Influencia de variables psicológicas en el rendimiento de jugadores amateurs de golf

Directory of Open Access Journals (Sweden)

Susana Irazusta

2006-01-01

Full Text Available El propósito de la presente investigación es analizar las variaciones del estado de ánimo intracompetición en los jugadores amateurs de golf, así como analizar la influencia que tiene el grado de confianza y otras variables motivacionales en el resultado de la competición. Se realizó la investigación con un total de 296 jugadores y se pasaron los cuestionarios a cada jugador en tres momentos, antes, durante y después de la competición.Una vez analizados los datos se constató que existen grandes fluctuaciones en el estado de ánimo de los jugadores y que el resultado esperado sufre modificaciones a lo largo de la competición.

Considerations for the Distribution of Antiarmor Weapons.

Science.gov (United States)

1985-05-20

German tank, used in mass formations, had been the decisive factor weapon.- The German Threat - "Blitzkrieg" The first German Tiger tank was not...also needed flank or rear shots to penetrate the armor of Panther and Tiger tanks at all but the closest ranges. A third self propelled antitank gun...Armored Threat In June 1944, the German Western Connand had 99 obsolete Pz III, 587 Pz IV, 290 Pz V (Panther) and only 63 Pz VI ( Tiger ) tanks available.20

Los mapas conceptuales y su influencia en lo afectivo-valorativo del estudiante para aprender biología

Directory of Open Access Journals (Sweden)

Acosta, Savier

2010-09-01

Full Text Available El objetivo de esta investigación fue identificar la influencia de los mapas conceptuales en lo afectivo-valorativo del estudiante para aprender Biología. Las teorías que sirvieron de apoyo las fueron Díaz y Hernández (2007, Novak (1998. La investigación se realizó en la Unidad Educativa José Antonio Almarza; fue de tipo experimental con un diseño cuasi-experimental. La población estuvo conformada por 468 estudiantes, de donde se seleccionó una muestra de 68 estudiantes (33 del grupo control y (35 el grupo experimental. La técnica utilizada para recolectar información fue la observación; el instrumento, una hoja de registro validado previamente por expertos en el área. Se aplicó la prueba t de student para establecer los grados de significancia entre el pre y postest, el análisis de los resultados se efectuó mediante la estadística descriptiva y la inferencial a través de frecuencias, porcentajes, media y la desviación estándar. Como resultados los mapas conceptuales aumentan en los estudiantes su responsabilidad para aprender Biología, respeto hacia la forma de pensar de los demás, valoración del trabajo en equipo, importancia para aprender Biología e incorporación de experiencias significativas del contexto. En conclusión que los mapas conceptuales ejercen influencia en el desarrollo de sentimientos y valores en los estudiantes para aprender dicha ciencia.

The effect of thai glutinous rice starch on the synthesis of lead zirconate (PbZrO3) nanofibers via the electrospinning method.

Science.gov (United States)

Nawanil, Chanisa; Vittayakorn, Wanwilai; Muanghua, Rangson; Niemcharoen, Surasak; Prachayawarakorn, Jutarat; Vittayakorn, Naratip

2013-05-01

This study synthesized blended lead zirconate (PbZrO3;PZ)/poly(ethylene oxide)(PEO)/Glutinous rice starch (GRS) nanofibers by the electrospinning method. A number of parameters such as the ratio between PEO and GRS and calcination temperature have been studied. The as-spun PZ/PEO/GRS composite and PZ fibers were characterized by TG-DTA, X-ray diffraction, FT-IR and SEM, respectively. SEM results showed that smooth and continuous fibers were obtained at the volume ratio of 10:2:1, PZ/PEO/GRS. After calcination of the as-spun PZ/PEO/GRS composite nanofibers at 650 degrees C for 4 h, PZ nanofibers with perovskite structure were obtained successfully. The fibers had a uniform and smooth surface without grain boundaries. However, when the calcination temperature increased to 750 degrees C and 850 degrees C, the fiber represented a necklace-like structure with grain boundaries arranged by grain to grain unit cell clusters.

Two-neutron stripping in ({sup 18}O, {sup 16}O) and (t,p) reactions

Energy Technology Data Exchange (ETDEWEB)

Cavallaro, M.; Agodi, A.; Carbone, D.; Cunsolo, A. [INFN - Laboratori Nazionali del Sud, Via S. Sofia 62, I-95125 Catania (Italy); Bondì, M.; Cappuzzello, F.; Nicolosi, D.; Tropea, S. [INFN - Laboratori Nazionali del Sud, Via S. Sofia 62, I-95125 Catania, Italy and Dipartimento di Fisica e Astronomia, Università di Catania, Via S. Sofia 64, I-95125 Catania (Italy); Borello-Lewin, T.; Rodrigues, M. R. D. [Instituto de Física - Universidade de São Paulo, Rua do Matão Travessa R Nr.187 CEP 05508-090 Cidade Universitária, São Paulo (Brazil); De Napoli, M. [INFN - Sezione di Catania, Via S. Sofia 64, I-95125 Catania (Italy); Garcia, V. N. [Instituto de Física, Universidade Federal Fluminense, Avenida Litoranea s/n, Gragoata, 24210-340, Niteroi, RJ (Brazil); Linares, R.; Lubian, J.; Paes, B. [Instituto de Física, Universidade Federal Fluminense, Avenida Litoranea s/n, Gragoata , 24210-340, Niteroi, RJ (Brazil); Foti, A. [Dipartimento di Fisica e Astronomia, Università di Catania, Via S. Sofia 64, I-95125 Catania, Italy and INFN - Sezione di Catania, Via S. Sofia 64, I-95125 Catania (Italy)

2014-11-11

The {sup 12}C({sup 18}O,{sup 16}O){sup 14}C reactions has been investigated at 84 MeV incident energy. The charged ejectiles produced in the reaction have been momentum analyzed and identified by the MAGNEX magnetic spectrometer. Q-value spectra have been extracted with an energy resolution of 160 keV (Full Width at Half Maximum) and several known bound and resonant states of {sup 14}C have been identified up to 15 MeV. In particular, excited states with dominant 2p - 4h configuration are the most populated. The absolute values of the cross sections have been extracted showing a striking similarity with those measured for the same transitions by (t,p) reactions. This indicates that the effect of the {sup 16}O core is negligible in the reaction mechanism.

Influencia de la buccalina ® en la concentración de streptococcus mutans en saliva total

OpenAIRE

Guardia Mendez, Gustavo

2015-01-01

Se realizó un estudio longitudinal en 28 niños de 6 años de edad para determinar la influencia de la BUCCALINA ® en la concentración de Streptococcus mutans en saliva total. Se midió la concentración de Streptococcus mutans en saliva total de todos los niños antes del experimento. 14 niños escogidos al azar recibieron la vacuna y 14 recibieron placebo. A los 37 días se midió la concentración de Streptococcus mutans en saliva total de todos los niños. Se usó la “t” de student para determinar l...

Influencia que tienen los programas de televisión infantil en los argumentos del juego simbólico

OpenAIRE

Jácome R., María Esther

2004-01-01

La influencia negativa de los programas televisivos dirigidos ó no a niños de 4 a 5 años determinan los argumentos del juego infantil, esta situación se evidencia en las repetidas manifestaciones de agresividad en el entorno escolar y familiar; en el uso de términos despectivos que descalifican y ridiculizan a compañeros y amigos; en los argumentos de conversación frente al juego; en el rechazo a las actividades físicas que impliquen movimiento; en la asunción de roles y postur...

CALIDAD DE VIDA, SÍNTOMAS SOMÁTICOS Y DOLOR EN LA ENFERMEDAD RENAL CRÓNICA: INFLUENCIA DE FACTORES

OpenAIRE

PERALES MONTILLA, CARMEN MARÍA

2017-01-01

El objetivo principal de esta Tesis fue valorar la interferencia que produce la Enfermedad Renal Crónica en el funcionamiento cotidiano del paciente en hemodiálisis. Para ello se han realizado cuatro estudios. Que valoran la Calidad de Vida Relacionada con la Salud (CVRS), la prevalencia de distintos tipos de síntomas somáticos y la sensibilidad al dolor. También se ha analizado la influencia de las variables psicológicas y sociales, sobre la CVRS y los síntomas somáticos. Asimismo, se ha com...

Influencia de la exposición gingival en la percepción estética de la sonrisa

OpenAIRE

Flores-Vignolo, Rosella; Meneses-López, Abraham; Liñán-Durán, Carlos

2014-01-01

Objetivo: Evaluar la influencia de los diferentes niveles de exposición gingival sobre la percepción estética de la sonrisa en pacientes y residentes del postgrado de Ortodoncia de la Universidad Peruana Cayetano Heredia (UPCH). Material y métodos: Diseño de corte observacional, descriptivo, transversal y comparativo. La muestra estuvo conformada por 36 pacientes y 35 residentes del postgrado de Ortodoncia de la UPCH. Para la ejecución del estudio se utilizó una fotografía del rostro durante ...

Influencia del estrés académico en el sistema binocular en universitarios de la UVA

OpenAIRE

López Gómez, Alejandra

2017-01-01

Objetivo: El estrés visual, tras trabajo prolongado en cerca, produce modificaciones en el sistema binocular. Esta hipótesis ha sido poco contrastada experimentalmente. El objetivo principal es medir la influencia del estrés académico en estudiantes universitarios de la UVA mediante la medida de la foria. Métodos: Se ha medido la foria en 22 estudiantes, con su corrección habitual, antes y después de un examen, mediante tres métodos: técnica de la varilla de Maddox, técnica de Von Graefe y...

Análisis cuantitativo en la influencia de la geomorfología en la estabilidad de taludes

OpenAIRE

Camacho Orozco, Sebastián; Ramos Cañón, Alfonso Mariano; Escobar Vargas, Jorge Alberto; Garzón González, Andrés Francisco

2017-01-01

La ocurrencia de deslizamientos está relacionada con unos factores que condicionan y otros que detonan los procesos de remoción en masa. Este trabajo contribuye a cuantificar la influencia de la geomorfología en la estabilidad de taludes como factor condicionante por medio de un análisis basado en el método de elementos finitos con un modelo constitutivo elastoplástico. Los análisis se realizaron a través de la simulación numérica de 9 modelos de taludes en tres dimensiones. Se obtuvo la vari...

Adrenocortical carcinoma with extension to the inferior vena cava and right atrium: 20-month-old girl with TP53 mutation

Directory of Open Access Journals (Sweden)

Terry L. Levin, MD

2015-01-01

Full Text Available A 20-month-old female presented with respiratory distress and a right adrenal mass extending into the inferior vena cava and right atrium. The mass was initially thought to be neuroblastoma. Pathology later revealed adrenocortical carcinoma. Inferior vena cava extension is far more common in adrenocortical carcinoma than neuroblastoma, and its presence should prompt clinical and laboratory evaluation for an adrenocortical tumor. The genetic findings in TP53 associated with this disease are discussed.

Influencia de las Emociones Negativas (Ansiedad, Depresión e Ira sobre la Eficacia de un Programa de Tratamiento Cognitivo Conductual de Deshabituación al Tabaco

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Francisco Javier Pérez-Pareja

2010-01-01

Full Text Available El objetivo de esta investigación es analizar la influencia de la ansiedad, la ira y la depresión, como emociones negativas, sobre el éxito en la aplicación de un tratamiento psicológico de carácter cognitivo-conductual para dejar de fumar. Estas tres respuestas emocionales fueron evaluadas en una muestra final de 180 personas antes de iniciar el tratamiento. Los resultados muestran que la depresión y la ira ejercen una importante influencia sobre el éxito terapéutico; es decir, habiendo dejado de fumar, mientras que la ansiedad no muestra efectos significativos. Sujetos con elevadas puntuaciones en depresión presentan importantes dificultades para finalizar el programa de manera exitosa, mientras que sujetos con puntuaciones elevadas en ira muestran una buena adherencia y un importante porcentaje de éxito en el tratamiento.

Influencia de los sistemas de información en las organizaciones

Directory of Open Access Journals (Sweden)

Diana Suárez López

2011-01-01

Full Text Available Resumen:El siguiente artículo tiene como objetivo resaltar el papel fundamental que tienen los sistemas de información en nuestras organizaciones, los impactos e influencia en cada área en las que implementamos estos sistemas para hacer más eficiente y eficaces los procesos administrativos, involucrando a todos los que conforman la organización. Por otra parte cómo puede una organización adquirir ventajas competitivas de las otras a través de los sistemas de información.Palabras Claves:Sistemas de Información, Tecnología, Organización, Internet, Competitividad.Abstract:The following article aims to highlight the fundamental role of information systems in our organizations, the impact and influence in each of the areas in which we implement these systems to create more efficient and effective administrative processes, involving all making up the organization. Moreover, this article also highlights how an organization can acquire competitive advantages from other organizations through information systems.Keywords:Information Systems, Technology, Organization, Internet, Competitiveness.

El liberalismo económico de la escuela de Salamanca y su influencia en el desarrollo institucional y organizacional

OpenAIRE

Vargas-Hernández, José G.; Castillo Girón, Víctor Manuel

2017-01-01

El objetivo de este trabajo es analizar la influencia que tiene el liberalismo económico de la llamada escuela de Salamanca en el desarrollo de las instituciones y organizaciones de su época. Se concluye que toda esta orientación de los teólogos morales de la escuela de Salamanca metidos a economistas, tratando asuntos de la economía, tenían como fi nalidad lograr un desarrollo institucional y organizacional que responde a las demandas de la época. Contrariamente al liberali...

Influencia de la religión en la toma de decisiones sobre sexualidad en estudiantes católicos practicantes

OpenAIRE

Juan Pablo Sanabria Mazo; Juliana Jiménez Sierra; Katherine Parra Blandón; María Alejandra Tordecilla Casallas

2016-01-01

Esta investigación cualitativa de diseño fenomenológico tuvo como objetivo comprender la influencia de la religión en la toma de decisiones sobre sexualidad en estudiantes católicos practicantes. La muestra, seleccionada intencionalmente, estuvo conformada por cuatro hombres y cuatro mujeres, de los cuales dos de cada sexo habían iniciado su vida sexual y dos no. Las entrevistas en profundidad se analizaron con el apoyo del programa Atlas.ti. Las motivaciones para no iniciar su vida sexual se...

Influencia de los bioplaguicidas en los parámetros de calidad de Calendula officinalis L. y Matricaria recutita L.

OpenAIRE

Sánchez Govín, Ester; Rivera Amita, María Magdalena; Carballo Guerra, Caridad

2010-01-01

INTRODUCCIÓN: Calendula officinalis L. y Matricaria recutita L. son dos especies medicinales de gran importancia económica y social en Cuba, su cultivo con frecuencia anual se ha visto afectado por la aparición de plagas y enfermedades, con el deterioro del rendimiento de masa vegetal y en su calidad. OBJETIVOS: determinar la influencia de los bioplaguicidas en los parámetros de calidad de Calendula officinalis L. y Matricaria recutita L. MÉTODOS: las drogas vegetales, fueron sometidas a trat...

Revitalisation of the control system of Shoshtanj TPP - Block 3 and TP 1 (Croatia)

International Nuclear Information System (INIS)

Shprem, Davor

1997-01-01

Block 3 of the Shoshtanj Thermal Power Plant was put into operation in 1960. With its 75 MW rated output on the Oerlikon turbine and the generation of 150 t/h of steam per each of the two Sulzer boilers fired by coal from the nearby Velenje mines, with Blocks I and 2, it was one of the biggest thermal power plants in Slovenia and in the former Yugoslavia. Although today, after the construction of much bigger Blocks 4 and 5, its share in the generation of electric and heating energy in the Republic of Slovenia is not so big, owing to its flexibility and reliability in operation, it is still a very important thermal power plant. Its importance conditioned numerous reconstructions with a view to increasing the efficiency of the block, extending its service life and increasing its ecological acceptability. A number of changes and upgradings were done, and ATM, Zagreb, today SIEMENS d.d. had an important share therein. This article deals with the revitalization of the control and monitoring system of Block 3 and Heating Station I of the Shoshtanj Thermal Power Plant. An emphasis is put on the installation of modern TELEPERM ME - AS220 EAI automation system and OS 525 operation and monitoring system. With the installation of the new automatic control system for Block 3 and TP I the main purpose of the reconstruction was achieved - the extension of the service life of the plant with an increased efficiency of its control. With the installation of the modem microprocessor automatic control system and the operation and monitoring system, a solid basis was ensured also for further upgrading and additions at later stages of reconstruction of Block 3, which would be finished by the complete replacement of the hydraulic oil control system and the relocation of the control of Block 3 and TP I in the new joint control room for Blocks 1, 2 and 3. The first stage of these extensive works starts with the reconstruction of the automatic control system of Blocks I and 2 which will be

Influencia del programa educativo sobre fisioterapia respiratoria en la práctica de la enfermera intensivista HNAAA, Chiclayo Perú 2011

OpenAIRE

Arrascue Lara, Sara Maribel

2012-01-01

La presente tesis estuvo guiada por el paradigma cuantitativo, persiguiendo como objetivo general, determinar la influencia del programa educativo sobre fisioterapia respiratoria en la práctica de las enfermeras intensivistas y como objetivos específicos, determinar el nivel de conocimientos sobre fisioterapia respiratoria, identificar las habilidades técnicas sobre este procedimiento respiratorio que tienen las enfermeras intensivistas antes y después de la aplicación del programa educativo ...

Comprehensive analysis of BRCA1, BRCA2 and TP53 germline mutation and tumor characterization: a portrait of early-onset breast cancer in Brazil.

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Dirce Maria Carraro

Full Text Available Germline mutations in BRCA1, BRCA2 and TP53 genes have been identified as one of the most important disease-causing issues in young breast cancer patients worldwide. The specific defective biological processes that trigger germline mutation-associated and -negative tumors remain unclear. To delineate an initial portrait of Brazilian early-onset breast cancer, we performed an investigation combining both germline and tumor analysis. Germline screening of the BRCA1, BRCA2, CHEK2 (c.1100delC and TP53 genes was performed in 54 unrelated patients <35 y; their tumors were investigated with respect to transcriptional and genomic profiles as well as hormonal receptors and HER2 expression/amplification. Germline mutations were detected in 12 out of 54 patients (22% [7 in BRCA1 (13%, 4 in BRCA2 (7% and one in TP53 (2% gene]. A cancer familial history was present in 31.4% of the unrelated patients, from them 43.7% were carriers for germline mutation (37.5% in BRCA1 and in 6.2% in the BRCA2 genes. Fifty percent of the unrelated patients with hormone receptor-negative tumors carried BRCA1 mutations, percentage increasing to 83% in cases with familial history of cancer. Over-representation of DNA damage-, cellular and cell cycle-related processes was detected in the up-regulated genes of BRCA1/2-associated tumors, whereas cell and embryo development-related processes were over-represented in the up-regulated genes of BRCA1/2-negative tumors, suggesting distinct mechanisms driving the tumorigenesis. An initial portrait of the early-onset breast cancer patients in Brazil was generated pointing out that hormone receptor-negative tumors and positive familial history are two major risk factors for detection of a BRCA1 germline mutation. Additionally, the data revealed molecular factors that potentially trigger the tumor development in young patients.

Study of the onset of deformation and shape coexistence in $^{46}$Ar via the inverse kinematics ($t,p$) reaction

CERN Multimedia

Reiter, P; Blazhev, A A; Nardelli, S; Stora, T; Tengborn, E A; Kruecken, R; Voulot, D; Korten, W; Srebrny, J; Clement, E; Lo bianco, G; Sorlin, O H; Habs, D; Fraile prieto, L M; Chapman, R; Nilsson, T; Diriken, J V J; Jenkins, D G; Wady, P T; Kroell, T; Patronis, N; Angus, L J; Iwanicki, J S

We plan to study states in $^{46}$ Ar via the (t,p) two-neutron transfer reaction in inverse kinematics in order to identify and characterize excited states and to gain insights into the onset of deformation and the possible occurrence of shape-coexistence in this region where the N = 28 shell closure may be weakening. The experiment will be performed using accelerated beams from REX-ISOLDE and the T-REX particle detector set-up inside MINIBALL. We request a total of 30 shifts + 3 shifts for beam commissioning.

Piperazine/N-methylpiperazine/N,N'-dimethylpiperazine as an Aqueous Solvent for Carbon Dioxide Capture

International Nuclear Information System (INIS)

Freeman, Stephanie A.; Chen, Xi; Nguyen, Thu; Rafique, Humera; Xu, Qing; Rochelle, Gary T.

2014-01-01

A blend of piperazine (PZ), N-methylpiperazine (MPZ) and N,N'-dimethylpiperazine (DMPZ) is described as a novel CO 2 capture solvent for aqueous absorption-stripping. This blend provides improved solid solubility and heat of absorption compared to concentrated PZ. No insolubility was observed for regions of high CO 2 loading, unlike PZ solvents. The blend performed like concentrated PZ in terms of CO 2 capacity and CO 2 absorption rate, both of which were more than double that of a traditional 7 molal (30 wt%) Monoethanolamine (MEA). Thermal equilibrium was established between the three constituent amines that increases the thermal stability compared to traditional blended solvents. The primary drawback of this novel solvent system is enhanced amine volatility at absorber conditions compared with both concentrated PZ and MEA. (authors)

Influencia de un sólido sobre el secado de mezclas líquidas parcialmente miscibles

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M. Vargas

2006-01-01

Full Text Available Un estudio experimental del secado convectivo de un sólido humedecido con una mezcla líquida que exhibe separación de fases durante el proceso de evaporación ha sido efectuado. Los experimentos fueron realizados en un secador de túnel a escala de laboratorio bajo diferentes condiciones de operación tales como temperatura y velocidad del gas así como diferentes composiciones de las mezclas líquidas. Se humedeció el sólido con mezclas binarias y ternarias, y se efectuó la evaporación a partir de la superficie libre líquida. Se utilizo como humedad el sistema binario agua-hexano y la mezcla ternaria agua-hexano-etanol, ambos involucrados en la extracción de biopesticida a partir de semillas de nim. Esferas de vidrio y semillas de nim trituradas fueron usadas como sólido. La densidad y el índice de distribución másico de las fases tienen gran influencia en el proceso de evaporación. Si la fase menos densa del hexano esta en exceso, la superficie es cubierta solamente por esta fase y no existe evaporación de la otra fase hasta que el índice de distribución másico es tal que la segunda fase es expuesta al gas. La presencia de un sólido poroso interrumpe este patrón y la fase más densa puede ser removida simultáneamente incluso a bajas fracciones de esta última. En este caso, también la forma en que el sólido fue humedecido tiene influencia en el proceso.

Malnutrición proteica como modelo de adversidad perinatal y su influencia sobre el desarrollo de capacidades cognitivas y socioemotivas

OpenAIRE

Belluscio, Laura María

2014-01-01

El desarrollo perinatal representa un período crítico en la vida de un individuo caracterizado por una sensibilidad extrema a experiencias e interacciones con el medio ambiente que originan efectos duraderos, y muchas veces permanentes, sobre la estructura y función del cerebro. La calidad del período embrionario como la calidad del ambiente y experiencias postnatales tempranas tienen una enorme influencia sobre el desarrollo emocional y cognitivo con consecuencias que se extienden durante to...

Influencia de las barreras a la exportación sobre el compromiso exportador y su incidencia en los resultados internacionales

OpenAIRE

Andrea Hurtado Ayala; Maribel Castillo Caicedo

2013-01-01

Rev.esc.adm.neg Este artículo, busca establecer la existencia de una relación entre las barreras a la exportación sobre el compromiso exportador de las empresas exportadoras colombianas y la influencia de dicho compromiso exportador sobre los resultados internacionales de estas empresas. Para cumplir estos objetivos, se parte de una muestra conformada por 270 empresas exportadoras colombianas y se estima un modelo de ecuaciones estructurales que comprueba que las barreras a la exportación...

La influencia del liderazgo sobre el aprendizaje. El papel mediador del contexto organizativo

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Susana Pérez López

2012-09-01

Full Text Available En este trabajo se analiza la relación existente entre liderazgo y aprendizaje organizativo. Con apoyo en investigaciones previas, se plantea un modelo cuya premisa básica es que la relación entre ambos conceptos es directa e indirecta, en la medida en que el liderazgo influye sobre el contexto organizativo en que se desarrolla el aprendizaje. El presente modelo teórico ha sido contrastado empíricamente a partir de una muestra de 162 empresas españolas, confirmándose la influencia del liderazgo sobre la cultura organizativa y el desarrollo de las políticas de recursos humanos, factores que, a su vez, influyen sobre el aprendizaje. Sin embargo, la relación directa entre liderazgo y aprendizaje organizativo no se confirma. Las implicaciones del estudio, limitaciones y futuras líneas de investigación son discutidas en el trabajo.

Combined human papillomavirus typing and TP53 mutation analysis in distinguishing second primary tumors from lung metastases in patients with head and neck squamous cell carcinoma.

Science.gov (United States)

Daher, Tamas; Tur, Mehmet Kemal; Brobeil, Alexander; Etschmann, Benjamin; Witte, Biruta; Engenhart-Cabillic, Rita; Krombach, Gabriele; Blau, Wolfgang; Grimminger, Friedrich; Seeger, Werner; Klussmann, Jens Peter; Bräuninger, Andreas; Gattenlöhner, Stefan

2018-06-01

In head and neck squamous cell carcinoma (HNSCC), the occurrence of concurrent lung malignancies poses a significant diagnostic challenge because metastatic HNSCC is difficult to discern from second primary lung squamous cell carcinoma (SCC). However, this differentiation is crucial because the recommended treatments for metastatic HNSCC and second primary lung SCC differ profoundly. We analyzed the origin of lung tumors in 32 patients with HNSCC using human papillomavirus (HPV) typing and targeted next generation sequencing of all coding exons of tumor protein 53 (TP53). Lung tumors were clearly identified as HNSCC metastases or second primary tumors in 29 patients, thus revealing that 16 patients had received incorrect diagnoses based on clinical and morphological data alone. The HPV typing and mutation analysis of all TP53 coding exons is a valuable diagnostic tool in patients with HNSCC and concurrent lung SCC, which can help to ensure that patients receive the most suitable treatment. © 2018 Wiley Periodicals, Inc.

Differential expression of ID4 and its association with TP53 mutation, SOX2, SOX4 and OCT-4 expression levels.

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Thais Fernanda de Almeida Galatro

Full Text Available Inhibitor of DNA Binding 4 (ID4 is a member of the helix-loop-helix ID family of transcription factors, mostly present in the central nervous system during embryonic development, that has been associated with TP53 mutation and activation of SOX2. Along with other transcription factors, ID4 has been implicated in the tumorigenic process of astrocytomas, contributing to cell dedifferentiation, proliferation and chemoresistance. In this study, we aimed to characterize the ID4 expression pattern in human diffusely infiltrative astrocytomas of World Health Organization (WHO grades II to IV of malignancy (AGII-AGIV; to correlate its expression level to that of SOX2, SOX4, OCT-4 and NANOG, along with TP53 mutational status; and to correlate the results with the clinical end-point of overall survival among glioblastoma patients. Quantitative real time PCR (qRT-PCR was performed in 130 samples of astrocytomas for relative expression, showing up-regulation of all transcription factors in tumor cases. Positive correlation was found when comparing ID4 relative expression of infiltrative astrocytomas with SOX2 (r = 0.50; p<0.005, SOX4 (r = 0.43; p<0.005 and OCT-4 (r = 0.39; p<0.05. The results from TP53 coding exon analysis allowed comparisons between wild-type and mutated status only in AGII cases, demonstrating significantly higher levels of ID4, SOX2 and SOX4 in mutated cases (p<0.05. This pattern was maintained in secondary GBM and further confirmed by immunohistochemistry, suggesting a role for ID4, SOX2 and SOX4 in early astrocytoma tumorigenesis. Combined hyperexpression of ID4, SOX4 and OCT-4 conferred a much lower (6 months median survival than did hypoexpression (18 months. Because both ID4 alone and a complex of SOX4 and OCT-4 activate SOX2 transcription, it is possible that multiple activation of SOX2 impair the prognosis of GBM patients. These observational results of associated expression of ID4 with SOX4 and OCT-4 may be used as a

Adquisición de Tecnologías de Información e Influencia institucional en Grandes Empresas en Iztapalapa

OpenAIRE

Regina Leal Güemez; Salvador T. Porras

2012-01-01

Las tecnologías de Información (TI) tienen una gran influencia en varios aspectos dentro de las organizaciones. Debido a ello los gobiernos de los países en vías de desarrollo han decidido impulsar su adopción con el objetivo de aumentar la productividad de las empresas, intensificar el uso y difusión de las TI, así como reducir l a brecha tecnológica respecto a los países desarrollados. E n este documento se reportan los resultados obtenidos de...

Influencia de la capacitación en el liderazgo de supervisores para la motivación de su personal

OpenAIRE

Martínez Marín, María Enriqueta

2003-01-01

En la presente investigación se analizó la influencia de la capacitación en el liderazgo de supervisores para la motivación de logro de su personal a cargo. Participaron 30 supervisores y 160 de personal operativo, de ambos géneros, personal de una empresa de servicios. Mediante selección aleatoria se dividió en dos grupos a los supervisores y se determinó como grupo A al que recibió capacitación en liderazgo y como grupo B al que recibió capacitación en seguridad ...

Influencia de los cambios en la profesión sobre la educación odontológica

OpenAIRE

Perdomo Sturniolo, Ivana Lorena

2017-01-01

El propósito de esta investigación será observar la influencia de los cambios producidos en la profesión y como afectaron en la forma de enseñar la odontología. Desde su creación hasta la actualidad, describiendo los hechos históricos más relevantes que llevaron a que se produzcan, y viendo cómo la enseñanza en las escuelas y Facultad de Odontología de la Universidad Nacional de la Plata han ido adaptándose a dichos cambios, haciendo hincapié en los últimos 50 años. Objetivos: Identificar los...

Influencia de la radiación solar en la salud de las personas en la ciudad de Moquegua

OpenAIRE

Rodríguez Ordoñez, Freddi Roland

2015-01-01

La presente investigación se realizó con el objetivo de comprobar la influencia de la radiación solar en la salud de las personas; para concretar esta investigación se aplicó las técnicas: análisis de contenido, fichaje y una encuesta, teniendo en cuenta instrumentos preparados de acuerdo a los objetivos específicos de la investigación. Se solicitó datos históricos de la radiación solar ultravioleta al Servicio Nacional de Meteorología e Hidrología del Perú (SENAMHI); al Hospital Regional de ...

Evaluación de la influencia en el desarrollo infantil de un proyecto de educación musical

OpenAIRE

Casas Lloret, Jara

2015-01-01

Esta investigación pretende determinar si hay influencias entre un proyecto de Educación Musical Infantil y el desarrollo integral de los niños/as que participan en el mismo, siguiendo el modelo de inteligencias múltiples de Gardner. Se evalúan niños/as entre 3 y 7 años de 7 colegios de Sevilla. Se hace un recorrido por el desarrollo humano, viendo sus diferentes dimensiones, centrándonos posteriormente en el desarrollo emocional. Se revisa la legislación actual para ver qué base teórica tien...

Influencia de parámetros clínicos sobre la severidad de la recesión gingival localizada

OpenAIRE

A. García-Rubio; A.L. Bujaldón-Daza; A. Rodríguez-Archilla

2015-01-01

Introducción: La recesión gingival localizada es un problema de salud bucodental fundamental porque su progresión conduce a la pérdida dentaria. El propósito de este trabajo fue evaluar la influencia de distintos parámetros clínicos sobre la recesión gingival localizada. Material y métodos: Se estudiaron 40 pacientes con recesión gingival localizada en los que se analizaron diversos parámetros clínicos relacionados con la recesión gingival. Para la comparación de variables cuantitativas se ut...

Plan de gestión integral del riego en la zona de influencia de la acequia de Tabacundo

OpenAIRE

Ochoa Mármol, Alexis Mauricio

2013-01-01

El presente producto consistió en la elaboración de plan integral de riego de la acequia Tabacundo el cual consta de un diagnóstico situacional en la zona de influencia, identificación de problemas, análisis del medio interno y externo, identificación de actores, planteamiento de la misión, visión, objetivos y estrategias para mejorar la gestión del agua de riego en la acequia Tabacundo dentro de los ámbitos socio-organizativo, técnico, económico productivo y ambiental. Este producto se ...

Influencias del capital humano y la violencia ciudadana sobre el crecimiento económico: Perú 1994-2014

OpenAIRE

Mendoza, Juan León; Universidad Nacional Mayor de San Marcos

2016-01-01

El artículo tiene como objetivo conocer los efectos del capital humano y de la violencia y la inseguridad sobre el crecimiento económico departamental peruano en el período 1994-2014. La regresión econométrica efectuada con el método de los Mínimos Cuadrados Ordinarios indica la existencia de una parcial convergencia interdepartamental. La tasa de convergencia es de 0,415. La acumulación de capital humano (aproximada por los años de estudio) muestra una influencia positiva sobre el crecimient...

EBV-Negative Monomorphic B-Cell Posttransplant Lymphoproliferative Disorder with Marked Morphologic Pleomorphism and Pathogenic Mutations in ASXL1, BCOR, CDKN2A, NF1, and TP53.

Science.gov (United States)

Bogusz, Agata M

2017-01-01

Posttransplant lymphoproliferative disorders (PTLDs) are a diverse group of lymphoid or plasmacytic proliferations frequently driven by Epstein-Barr virus (EBV). EBV-negative PTLDs appear to represent a distinct entity. This report describes an unusual case of a 33-year-old woman that developed a monomorphic EBV-negative PTLD consistent with diffuse large B-cell lymphoma (DLBCL) 13 years after heart-lung transplant. Histological examination revealed marked pleomorphism of the malignant cells including nodular areas reminiscent of classical Hodgkin lymphoma (cHL) with abundant large, bizarre Hodgkin-like cells. By immunostaining, the malignant cells were immunoreactive for CD45, CD20, CD79a, PAX5, BCL6, MUM1, and p53 and negative for CD15, CD30, latent membrane protein 1 (LMP1), and EBV-encoded RNA (EBER). Flow cytometry demonstrated lambda light chain restricted CD5 and CD10 negative B-cells. Fluorescence in situ hybridization studies (FISH) were negative for cMYC , BCL2, and BCL6 rearrangements but showed deletion of TP53 and monosomy of chromosome 17. Next-generation sequencing studies (NGS) revealed numerous genetic alterations including 6 pathogenic mutations in ASXL1, BCOR, CDKN2A, NF1, and TP53 (x2) genes and 30 variants of unknown significance (VOUS) in ABL1, ASXL1, ATM, BCOR, BCORL1, BRNIP3, CDH2, CDKN2A, DNMT3A, ETV6, EZH2, FBXW7, KIT, NF1, RUNX1, SETPB1, SF1, SMC1A, STAG2, TET2, TP53, and U2AF2.

EBV-Negative Monomorphic B-Cell Posttransplant Lymphoproliferative Disorder with Marked Morphologic Pleomorphism and Pathogenic Mutations in ASXL1, BCOR, CDKN2A, NF1, and TP53

Directory of Open Access Journals (Sweden)

Agata M. Bogusz

2017-01-01

Full Text Available Posttransplant lymphoproliferative disorders (PTLDs are a diverse group of lymphoid or plasmacytic proliferations frequently driven by Epstein-Barr virus (EBV. EBV-negative PTLDs appear to represent a distinct entity. This report describes an unusual case of a 33-year-old woman that developed a monomorphic EBV-negative PTLD consistent with diffuse large B-cell lymphoma (DLBCL 13 years after heart-lung transplant. Histological examination revealed marked pleomorphism of the malignant cells including nodular areas reminiscent of classical Hodgkin lymphoma (cHL with abundant large, bizarre Hodgkin-like cells. By immunostaining, the malignant cells were immunoreactive for CD45, CD20, CD79a, PAX5, BCL6, MUM1, and p53 and negative for CD15, CD30, latent membrane protein 1 (LMP1, and EBV-encoded RNA (EBER. Flow cytometry demonstrated lambda light chain restricted CD5 and CD10 negative B-cells. Fluorescence in situ hybridization studies (FISH were negative for cMYC, BCL2, and BCL6 rearrangements but showed deletion of TP53 and monosomy of chromosome 17. Next-generation sequencing studies (NGS revealed numerous genetic alterations including 6 pathogenic mutations in ASXL1, BCOR, CDKN2A, NF1, and TP53(x2 genes and 30 variants of unknown significance (VOUS in ABL1, ASXL1, ATM, BCOR, BCORL1, BRNIP3, CDH2, CDKN2A, DNMT3A, ETV6, EZH2, FBXW7, KIT, NF1, RUNX1, SETPB1, SF1, SMC1A, STAG2, TET2, TP53, and U2AF2.

Entretenimiento, espiritualidad, familia y la influencia de pares universitarios en el consumo de drogas

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Aydee Rivera de Parada

2015-01-01

Full Text Available El objetivo del estudio fue examinar la relación de los factores socio-culturales de familia, entretenimiento y espiritualidad y la influencia de pares y el consumo de drogas entre los estudiantes universitarios. Estudio es multicéntrico, con un diseño transversal, participaron 250 estudiantes de una universidad en San Salvador, El Salvador. Se realizaron análisis porcentuales, pruebas de asociación y correlación. Se encontró que los factores socioculturales de familia y espiritualidad podrían estar protegiendo ya que la mayoría 80% valoran positivamente estos dos valores. La mayoría de estudiantes consumen muy poco drogas ilícitas, marihuana 2%, cocaína 1% y anfetaminas 1.2%; pero consume más las drogas lícitas alcohol 42%, tabaco 21%.

71Ga and 73Ga levels as observed in the (t,p) reaction

International Nuclear Information System (INIS)

Vergnes, M.N.; Rotbard, G.; Guilbaut, F.; Ardouin, D.; Lebrun, C.

1978-01-01

A study of the (t,p) reaction on the two stable Ga isotopes has been performed. The reaction protons were analyzed in a Q3D spectrometer with a resulting energy resolution approximately 18 keV. Levels up to about 3 MeV excitation energy in 71 Ga and 2.75 MeV in 73 Ga were measured with 11 new levels observed in the first case and 18 in the second. The angular distributions have been compared to pure distributions observed in the 72 Ge(t,p) and 74 Ge(t,p) reactions at the same energy and found to correspond mostly to pure angular momentum (L) transfer although mixing of L's is allowed. A number of new spins assignments are made for Ga levels and the results are used to discuss the spin of 73 Znsub(g.s.). The striking splitting of the L=0 strength in three approximately equal components, observed in 73 Ga, strongly supports a transition in nuclear deformation between N=40 and 42

TP53 mutations, human papilloma virus DNA and inflammation markers in esophageal squamous cell carcinoma from the Rift Valley, a high-incidence area in Kenya

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Martel-Planche Ghislaine

2011-10-01

Full Text Available Abstract Background Squamous Cell Carcinoma of Esophagus is one of the most common malignancies in both men and women in eastern and south-eastern Africa. In Kenya, clinical observations suggest that this cancer is frequent in the Rift Valley area. However, so far, there has been no report on the molecular characteristics of esophageal squamous cell carcinoma (ESCC in this area. Results We have analyzed TP53 mutations, the presence of human papilloma virus (HPV DNA and expression of inflammation markers Cyclooxygenase 2 (Cox-2 and Nitrotyrosine (NTyR in 28 cases (13 males and 15 females of archived ESCC tissues collected at the Moi Teaching and Referral Hospital in Eldoret, Kenya. Eleven mutations were detected in TP53 exons 5 to 8 (39%. All ESCC samples were negative for HPV 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 70, 73 and 82. Immunohistochemical analysis of Cox-2 and NTyR showed a low proportion of positive cases (17.4% and 39.1%, respectively. No association between the above markers and suspected risk factors (alcohol or tobacco use, hot tea drinking, use of charcoal for cooking was found. Conclusion Our findings suggest that mechanisms of esophageal carcinogenesis in eastern Africa might be different from other parts of the world. Low prevalence of TP53 mutation compared with other intermediate or high incidence areas of the world highlights this hypothesis. Our data did not support a possible ole of HPV in this series of cases. Further studies are needed to assess and compare the molecular patterns of ESCC from Kenya with those of high-incidence areas such as China or Central Asia.

The Suitability of Pozzolan as Admixing Aggregate for Fe0-Based Filters

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Arnaud Igor Ndé-Tchoupé

2018-04-01

Full Text Available Continuous gravity-fed column experiments using the methylene blue (MB discoloration method were performed to characterize the suitability of a pozzolan (PZ specimen as alternative admixing aggregate for metallic iron filters (Fe0-filters. Investigated systems were: (i pure sand, (ii pure PZ, (iii pure Fe0, (iv Fe0/sand, (v Fe0/PZ, and (vi Fe0/sand/PZ. The volumetric proportion of Fe0 was 25%. The volumetric proportions of the Fe0/sand/PZ system was 25/45/30. The initial MB concentration was 2.0 mg·L−1, 6.0 g of Fe0 was used, and the experiments lasted for 46 days. The individual systems were fed with 3.9 to 8.4 L (7.80 to 16.69 mg of MB and were characterized by the time-dependent changes of: (i the pH value, (ii the iron breakthrough, (iii the MB breakthrough, and (iv the hydraulic conductivity. Results showed that the Fe0/sand/PZ system was the most efficient. This ternary system was also the most permeable and therefore the most sustainable. The suitability of MB as a powerful operative indicator for the characterization of processes in the Fe0/H2O system was confirmed. The tested PZ is recommended as an alternative material for efficient but sustainable Fe0 filters.

The interchangeability of global positioning system and semiautomated video-based performance data during elite soccer match play.

Science.gov (United States)

Harley, Jamie A; Lovell, Ric J; Barnes, Christopher A; Portas, Matthew D; Weston, Matthew

2011-08-01

In elite-level soccer, player motion characteristics are commonly generated from match play and training situations using semiautomated video analysis systems and global positioning system (GPS) technology, respectively. Before such data are used collectively to quantify global player load, it is necessary to understand both the level of agreement and direction of bias between the systems so that specific interventions can be made based on the reported results. The aim of this report was to compare data derived from both systems for physical match performances. Six elite-level soccer players were analyzed during a competitive match using semiautomated video analysis (ProZone® [PZ]) and GPS (MinimaxX) simultaneously. Total distances (TDs), high speed running (HSR), very high speed running (VHSR), sprinting distance (SPR), and high-intensity running distance (HIR; >4.0 m·s(-1)) were reported in 15-minute match periods. The GPS reported higher values than PZ did for TD (GPS: 1,755.4 ± 245.4 m; PZ: 1,631.3 ± 239.5 m; p < 0.05); PZ reported higher values for SPR and HIR than GPS did (SPR: PZ, 34.1 ± 24.0 m; GPS: 20.3 ± 15.8 m; HIR: PZ, 368.1 ± 129.8 m; GPS: 317.0 ± 92.5 m; p < 0.05). Caution should be exercised when using match-load (PZ) and training-load (GPS) data interchangeably.

Screening for germline BRCA1, BRCA2, TP53 and CHEK2 mutations in families at-risk for hereditary breast cancer identified in a population-based study from Southern Brazil

Directory of Open Access Journals (Sweden)

Edenir Inêz Palmero

2016-01-01

Full Text Available Abstract In Brazil, breast cancer is a public health care problem due to its high incidence and mortality rates. In this study, we investigated the prevalence of hereditary breast cancer syndromes (HBCS in a population-based cohort in Brazils southernmost capital, Porto Alegre. All participants answered a questionnaire about family history (FH of breast, ovarian and colorectal cancer and those with a positive FH were invited for genetic cancer risk assessment (GCRA. If pedigree analysis was suggestive of HBCS, genetic testing of the BRCA1, BRCA2, TP53, and CHEK2 genes was offered. Of 902 women submitted to GCRA, 214 had pedigrees suggestive of HBCS. Fifty of them underwent genetic testing: 18 and 40 for BRCA1/BRCA2 and TP53 mutation screening, respectively, and 7 for CHEK2 1100delC testing. A deleterious BRCA2 mutation was identified in one of the HBOC probands and the CHEK2 1100delC mutation occurred in one of the HBCC families. No deleterious germline alterations were identified in BRCA1 or TP53. Although strict inclusion criteria and a comprehensive testing approach were used, the suspected genetic risk in these families remains unexplained. Further studies in a larger cohort are necessary to better understand the genetic component of hereditary breast cancer in Southern Brazil.

Development and validation of an ICP-MS method for the determination of elemental impurities in TP-6076 active pharmaceutical ingredient (API) according to USP 〈232〉/〈233〉.

Science.gov (United States)

Chahrour, Osama; Malone, John; Collins, Mark; Salmon, Vrushali; Greenan, Catherine; Bombardier, Amy; Ma, Zhongze; Dunwoody, Nick

2017-10-25

The new guidelines of the United States pharmacopeia (USP), European pharmacopeia (EP) and international conference on harmonization (ICH) regulating elemental impurities limits in pharmaceuticals signify the end of unspecific analysis of metals as outlined in USP 〈231〉. The new guidelines specify both daily doses and concentration/limits of elemental impurities in pharmaceutical final products, active pharmaceutical ingredients (API) and excipients. In chapter USP 〈233〉 method implementation, validation and quality control during the analytical process are described. We herein report the use of a stabilising matrix that overcomes low spike recovery problem encountered with Os and allows the determination of all USP required elemental impurities (As, Cd, Hg, Pb, V, Cr, Ni, Mo, Cu, Pt, Pd, Ru, Rh, Os and Ir) in a single analysis. The matrix was used in the validation of a method to determine elemental impurities in TP-6076 active pharmaceutical ingredient (API) by ICP-MS according to the procedures defined in USP〈233〉 and to GMP requirements. This validation will support the regulatory submission of TP-6076 which is a novel tetracycline analogue effective against the most urgent multidrug-resistant gram-negative bacteria. Evaluation of TP-6076 in IND-enabling toxicology studies has led to the initiation of a phase 1 clinical trial. Copyright © 2017 Elsevier B.V. All rights reserved.

Diferencias en memoria entre hombres y mujeres jóvenes sanos: influencia de las variaciones naturales de las hormonas sexuales

OpenAIRE

Otero Dadín, Cecilia

2013-01-01

El objetivo general del presente trabajo es analizar las diferencias entre sexos y la influencia de los ciclos naturales de las hormonas sexuales en memoria. Se evaluó con pruebas de memoria a 36 hombres y 39 mujeres jóvenes sanos en dos ocasiones, coincidentes con dos fases hormonales, bien del ciclo diurno de la testosterona en los varones o bien del ciclo menstrual en las mujeres. Se observó una ventaja masculina en memoria auditiva inmediata y en memoria de trabajo para material verba...

Influencia de las Redes Sociales en la Integración Social de jóvenes con Trastornos del Espectro Autista.

OpenAIRE

Legorburu-Fernández, Idoia

2013-01-01

El presente estudio versa sobre la influencia que ejercen las Redes Sociales (RRSS) en la socialización de las personas con Trastorno del Espectro Autista (TEA). Se investiga si mediante las nuevas herramientas existentes en la red los jóvenes con TEA podrían mejorar sus lazos sociales. Más concretamente, tiene como objeto conocer el uso que le dan dichos alumnos a las plataformas sociales, para poder optimizar, posteriormente, las metodologías que utilizan los profesionales en el entrenamien...

Integral blow moulding for cycle time reduction of CFR-TP aluminium contour joint processing

Science.gov (United States)

Barfuss, Daniel; Würfel, Veit; Grützner, Raik; Gude, Maik; Müller, Roland

2018-05-01

Integral blow moulding (IBM) as a joining technology of carbon fibre reinforced thermoplastic (CFR-TP) hollow profiles with metallic load introduction elements enables significant cycle time reduction by shortening of the process chain. As the composite part is joined to the metallic part during its consolidation process subsequent joining steps are omitted. In combination with a multi-scale structured load introduction element its form closure function enables to pass very high loads and is capable to achieve high degrees of material utilization. This paper first shows the process set-up utilizing thermoplastic tape braided preforms and two-staged press and internal hydro formed load introduction elements. Second focuses on heating technologies and process optimization. Aiming at cycle time reduction convection and induction heating in regard to the resulting product quality is inspected by photo micrographs and computer tomographic scans. Concluding remarks give final recommendations for the process design in regard to the structural design.

DNA impedance biosensor for detection of cancer, TP53 gene mutation, based on gold nanoparticles/aligned carbon nanotubes modified electrode.

Science.gov (United States)

Fayazfar, H; Afshar, A; Dolati, M; Dolati, A

2014-07-11

For the first time, a new platform based on electrochemical growth of Au nanoparticles on aligned multi-walled carbon nanotubes (A-MWCNT) was developed for sensitive lable-free DNA detection of the TP53 gene mutation, one of the most popular genes in cancer research. Electrochemical impedance spectroscopy (EIS) was used to monitor the sequence-specific DNA hybridization events related to TP53 gene. Compared to the bare Ta or MWCNT/Ta electrodes, the synergistic interactions of vertically aligned MWCNT array and gold nanoparticles at modified electrode could improve the density of the probe DNA attachment and resulting the sensitivity of the DNA sensor greatly. Using EIS, over the extended DNA concentration range, the change of charge transfer resistance was found to have a linear relationship in respect to the logarithm of the complementary oligonucleotides sequence concentrations in the wide range of 1.0×10(-15)-1.0×10(-7)M, with a detection limit of 1.0×10(-17)M (S/N=3). The prepared sensor also showed good stability (14 days), reproducibility (RSD=2.1%) and could be conveniently regenerated via dehybridization in hot water. The significant improvement in sensitivity illustrates that combining gold nanoparticles with the on-site fabricated aligned MWCNT array represents a promising platform for achieving sensitive biosensor for fast mutation screening related to most human cancer types. Copyright © 2014. Published by Elsevier B.V.

Estudio de la influencia de la automatización en el proyecto arquitectónico

Directory of Open Access Journals (Sweden)

Millán-Anglés, S.

2014-06-01

Full Text Available Integrated automation may have a fundamental role in the architecture of the future. However, until today the home automation is not a factor taken into account from the architectural design and this impedes this discipline to be generalised in order to reshape the future of these new buildings. Therefore, there is a need for a thorough study of the influence on constructive and projective aspects that exist in domotics and home automation. To develop this research there have been isolated the constructive and projective conditions detected in home automation, conditions that should be taken into account from the design stage to promote the implementation of new technologies in buildings in order to ensure the viability of these new facilities. This article analyzes, therefore, the main constructive and projective considerations being detected in the study of the influence of home automation on how to design an architectural project.La domótica integrada puede terminar jugando un papel fundamental en la arquitectura del futuro. Sin embargo, hasta hoy la automatización doméstica no es un factor tenido en cuenta desde el proyecto arquitectónico y este hecho hace que esta disciplina no se generalice para replantear el futuro de estas nuevas construcciones. Por ello, se plantea la necesidad de un estudio profundo de la influencia sobre los aspectos constructivos y proyectuales que existen en la domotización y automatización de viviendas. Para desarrollar esta investigación se han aislado los posibles condicionantes de la automatización, condicionantes que deberían tenerse en cuenta desde la fase proyecto para fomentar la implantación de las nuevas tecnologías en los edificios y así garantizar la viabilidad de estas nuevas instalaciones. El presente artículo analiza, por tanto, las principales consideraciones de carácter constructivo y proyectual que se están detectando en el estudio de la influencia de la domótica sobre la forma de

Influencia do indice de massa corporal no equilibrio e na configuracao plantar em obesos adultos

Directory of Open Access Journals (Sweden)

Liu Chiao Yi

2014-01-01

Full Text Available INTRODUÇÃO: A obesidade é uma doença crônica degenerativa multifatorial que pode levar a alterações do sistema musculoesquelético, como mudança do centro de gravidade e sobrecarga mecânica sobre os membros inferiores. OBJETIVOS: Correlacionar o índice de massa corporal (IMC com o equilíbrio corporal e verificar associação entre o IMC e a configuração plantar. MÉTODOS: Foram avaliados 30 obesos, de ambos os gêneros, com IMC maior ou igual a 30 Kg/m². Inicialmente, os voluntários foram submetidos às avaliações de medidas antropométricas a fim de calcular o valor do IMC. Em seguida, foram submetidos ao teste de equilíbrio corporal estático Balance Error Scoring System (BESS e a plantigrafia para a identificação da impressão plantar. Por meio do método de Viladot, os voluntários foram classificados em grupos: pé plano (GPP, pé cavo (GPC e pé neutro (GPN. A correlação entre as variáveis IMC e BESS foi calculada por meio do coeficiente de correlação linear de Pearson e associação entre o IMC e a configuração plantar foi realizada por meio da análise de variância (Anova. Para todas as análises, nível de significância considerado foi p < 0,05. RESULTADOS: Os valores da correlação entre o IMC e o BESS foram r = - 0,1 e p = 0,59. Os valores da associação do IMC entre GPN - GPP; GPN - GPC; GPP - GPC foram respectivamente: p = 0,76; p = 0,001; p = 0,07. CONCLUSÃO: O índice de massa corporal de adultos obesos não influencia o equilíbrio corporal, porém influencia na configuração plantar.

A resolvase-like protein is requered for the site-specific integration of the temperate lactococcal bacteriophage TP901-1

DEFF Research Database (Denmark)

Christiansen, Bettina; Brøndsted, Lone; Vogensen, Finn K.

1996-01-01

upstream of attP. The N-terminal 150 to 1180 amino acids of Orf1 showed 38 to 44% similarity to the resolvase group of site-specific integrases, while no similarity to know proteins was found in the C-terminal end. Bacteriophage 'TP901-1 therefore contains a unique integration system that does not resemble...... the Int class of site-specific integrases usually found in temperate bacteriophages. The constructed integration vector, pBC170, integrates into the chromosomal attachment site very efficiently and forms stable transformants with a frequency corresponding to 20% of the transformation efficiency....

Las influencias de Bakunin y Kropotkin sobre el movimiento libertario español

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Frank Mintz

2010-04-01

Full Text Available El autor hace hincapié en el peso de la visión bakuninista en el inicio del movimiento sindical español y de paso trata de trazar líneas de separación entre el sindicalismo libertario y el movimiento anarquista. Insiste también en la profunda influencia de Kropotkin entre la militancia de la CNT.El autor considera que el movimiento libertario español (con su honda tradición sindical es inseparable de Bakunin y Kropotkin.Palabras claves:  CNT, Movimiento libertario, Bakunin, Kropotkin____________________ABSTRACT:The author emphasizes the weight of Bakunin's vision in starting the Spanish trade union movement and by the way he tries to draw lines of separation between libertarian syndicalism and anarchist movement. He underlines also the profound influence of Kropotkin among CNT activists. The author believes that the Spanish libertarian movement (with its deep tradition of unionism is inseparable from Bakunin and Kropotkin. Keywords: CNT, libertarian movement, Bakunin, Kropotkin

Assembly of the U5 snRNP component PRPF8 is controlled by the HSP90/R2TP chaperones

Czech Academy of Sciences Publication Activity Database

Malinová, Anna; Cvačková, Zuzana; Matějů, Daniel; Hořejší, Zuzana; Abeza, C.; Vandermoere, F.; Bertrand, E.; Staněk, David; Verheggen, C.

2017-01-01

Roč. 216, č. 6 (2017), s. 1579-1596 ISSN 0021-9525 R&D Projects: GA ČR GPP301/12/P425; GA ČR GA15-00790S; GA ČR(CZ) GA14-34264S; GA MŠk LO1419 Institutional support: RVO:68378050 Keywords : dominant retinitis-pigmentosa * splicing factor prp8 * rna-polymerase-ii * structural basis * spliceosomal snrnps * coiled bodies * cajal bodies * r2tp complex * mutations * biogenesis Subject RIV: EB - Genetics ; Molecular Biology OBOR OECD: Biochemistry and molecular biology Impact factor: 7.955, year: 2016

Influencia de curso pre cálculo en ecuaciones diferenciales y desarrollo de pensamiento variacional

Directory of Open Access Journals (Sweden)

Mawency Vergel Ortega

2018-01-01

Full Text Available La investigación sigue un enfoque cuantitativo con apoyo del enfoque cualitativo, descriptivo, diseño cuasi experimental. En esta investigación se plantearon los objetivos: estudiar si mejoran las competencias en pensamiento variacional y didácticas de los estudiantes con la utilización de curso precálculo utilizando plataforma virtual respecto a enseñanza tradicional; examinar la influencia en desarrollo del pensamiento matemático y su enseñanza; así como analizar qué tipología de alumnos obtiene mejores resultados en relación a su nivel de competencia digital. Se han obtenido los siguientes avances: el grupo experimental ha obtenido una mejora estadísticamente significativa de sus competencias interpretativas y de pensamiento matemático respecto al grupo control.

INFLUENCIA DEL MANEJO DE LOS RESIDUOS SÓLIDOS EN EL ENTORNO AMBIENTAL DEL CAMPUS UNIVERSITARIO PERAYOC DE LA UNSAAC 2014- CUSCO

OpenAIRE

FRANCO MENDEZ, CARLOS REYNALDO

2015-01-01

MEDICIÓN Y CARACTERIZACIÓN DE LOS RESIDUOS SÓLIDOS UNIDADES DE ESTUDIO CARACTERIZACIÓN DE RESIDUOS CARACTERIZACIÓN Y EFICIENCIA DEL MANEJO DE RESIDUOS RESULTADOS DE LA OBSERVACIÓN SOBRE EL MANEJO DE LOS RESIDUOS SÓLIDOS EN LA UNSAAC GRADO DE INFLUENCIA DEL MANEJO DE RESIDUOS SOBRE EL IMPACTO AMBIENTAL IMPACTOS AMBIENTALES GENERADOS POR LA ACTIVIDAD DE MANEJO DE RESIDUOS EN LA UNSAAC. FORMULACIÓN DE LA MATRIZ DE IMPORTANCIA MATRIZ DE IMPORTANCIA: VALORACIÓN DE IMPACTOS AMBIENTALES

CO2 Capture by Absorption with Potassium Carbonate

Energy Technology Data Exchange (ETDEWEB)

Rochelle, Gary T; Seibert, Frank; Closmann, Fred; Cullinane, Tim; Davis, Jason; Goff, George; Hilliard, Marcus; McLees, John; Plaza, Jorge M; Sexton, Andrew; Wagener, David Van; Zu, Qing; Veawab, Amornvadee; Nainar, Manjula

2007-08-31

The objective of this work is to improve the process for CO{sub 2} capture by alkanolamine absorption/stripping by developing an alternative solvent, aqueous K{sub 2}CO3 promoted by piperazine (PZ). Pilot plant testing was performed in a 16.8-inch ID absorber and stripper with recirculation of air and CO{sub 2}. Three solvents (7 m MEA, 5 m K{sup +}/2.5 m PZ, and 6.4 m K{sup +}/1.6 m PZ) were tested in four campaigns with three different absorber packings. Pilot plant testing established that 5 m K{sup +}/2.5 m PZ requires two times less packing than 7 m MEA and three times less packing than 6.4 m K{sup +}/1.6 m PZ. A rigorous model of the thermodynamics and mass transfer was developed in the RateSep{trademark} block of AspenPlus{reg_sign}. The double matrix stripper reduces energy consumption by 5 to 15%. The best K{sup +}/PZ solvent, 4 m K{sup +}/4 m PZ, and the best process configuration, double matrix stripper with a double intercooled absorber, requires equivalent work of 40 kJ/mole CO{sub 2} to produce CO{sub 2} at 10 MPa. Inhibitor A is effective at reducing oxidative degradation over a wide range of metal concentrations and solvent types. Piperazine is resistant to oxidative degradation catalyzed by dissolved iron, but it oxidizes at rates comparable to monoethanolamine (MEA) in the presence of dissolved copper. The thermal degradation of MEA becomes significant at 120 C, but loaded piperazine solutions appear to be resistant to thermal degradation up to 135 C. The vapor pressure of PZ over typical lean solution at 40 C will be less than 25 ppm, which is less than the 40 ppm expected for MEA. Significant problems with foaming were encountered and alleviated by antifoamants in the pilot plant campaigns with K{sup +}/PZ. Potassium sulfate is not very soluble in 4 m K{sup +}/4 m PZ, so SO{sub 2} absorption and oxidation to sulfate in the bottom of the absorber may require operation with a slurry of potassium sulfate solids.

Synthesis and characterization of a porphyrazine-Gd(III) MRI contrast agent and in vivo imaging of a breast cancer xenograft model.

Science.gov (United States)

Trivedi, Evan R; Ma, Zhidong; Waters, Emily A; Macrenaris, Keith W; Subramanian, Rohit; Barrett, Anthony G M; Meade, Thomas J; Hoffman, Brian M

2014-01-01

Porphyrazines (Pz), or tetraazaporphyrins, are being studied for their potential use in detection and treatment of cancer. Here, an amphiphilic Cu-Pz-Gd(III) conjugate has been prepared via azide-alkyne Huisgen cycloaddition or 'click' chemistry between an azide functionalized Pz and alkyne functionalized DOTA-Gd(III) analog for use as an MRI contrast agent. This agent, Cu-Pz-Gd(III), is synthesized in good yield and exhibits solution-phase ionic relaxivity (r1  = 11.5 mM(-1) s(-1)) that is approximately four times higher than that of a clinically used monomeric Gd(III) contrast agent, DOTA-Gd(III). Breast tumor cells (MDA-MB-231) associate with Cu-Pz-Gd(III) in vitro, where significant contrast enhancement (9.336 ± 0.335 contrast-to-noise ratio) is observed in phantom cell pellet MR images. This novel contrast agent was administered in vivo to an orthotopic breast tumor model in athymic nude mice and MR images were collected. The average T1 of tumor regions in mice treated with 50 mg kg(-1) Cu-Pz-Gd(III) decreased relative to saline-treated controls. Furthermore, the decrease in T1 was persistent relative to mice treated with the monomeric Gd(III) contrast agent. An ex vivo biodistribution study confirmed that Cu-Pz-Gd(III) accumulates in the tumors and is rapidly cleared, primarily through the kidneys. Differential accumulation and T1 enhancement by Cu-Pz-Gd(III) in the tumor's core relative to the periphery offer preliminary evidence that this agent would find application in the imaging of necrotic tissue. Copyright © 2014 John Wiley & Sons, Ltd.

Influencia del consumo de cannabis en la enfermedad periodontal: una Scoping Review

Directory of Open Access Journals (Sweden)

Matias Mederos

Full Text Available Resumen: Recientes publicaciones plantean la posible asociación del cannabis (Cannabis sativa con el desarrollo de la periodontitis. Objetivo: Analizar la influencia del consumo de cannabis en la enfermedad periodontal, relevando la evidencia disponible e identificando las variables asociadas en los estudios . Material y método: se realizó una Scoping Review a partir de una búsqueda estructurada en PubMed/ MEDLINE, Science Direct, LILACS, SciELO hasta mayo de 2017 . Resultados: Estudios “In vivo” mostraron una mayor pérdida ósea en animales expuestos a Cannabis. Los casos clínicos muestran que el uso crónico de cannabis puede resultar en agrandamientos gingivales y periodontitis crónica severa localizada. Los estudios epidemiológicos demuestran una asociación estadística entre Cannabis y periodontitis. Conclusiones: El consumo del Cannabis parece estar asociado con una mayor presencia de periodontitis. Sin embargo, se desconoce el mecanismo específico por el cual actúa en los tejidos gingivales.

Axially substituted silicon (IV) tetrapyrazinoporphyrazines: Synthesis, characterization and investigation of photophysicochemical properties

Energy Technology Data Exchange (ETDEWEB)

Jung, Chang Young; Park, Jong Min; Song, Cheol Jun; Jaung, Jae Yun, E-mail: jjy1004@hanyang.ac.kr

2016-02-15

A series of silicon tetrapyrazinoporphyrazine (SiPz) complexes, conjugated with various substituents at their axial positions, were synthesized into non-aggregated structures in organic solvents. The UV–vis spectra of the SiPz complexes maintained a strong and sharp Q-band, which is an archetypal characteristic of non-aggregated phthalocyanines (Pcs), in various organic solvents under high concentrations. In addition, the SiPz complexes with axial alkyl groups had high fluorescence quantum yields and singlet oxygen quantum yields. However, the SiPz complexes with axial aniline groups had very low fluorescence quantum yields and singlet oxygen quantum yields due to the quenching ability of the aniline group.

Influencia del uso de mapas conceptuales en la construcción de la habilidad. Clasificación en Ciencias Naturales

OpenAIRE

Amaya Moyano, Blanca Lilia

2003-01-01

Tesis (Maestría en Educación y Desarrollo Humano). Universidad de Manizales. Facultad de Ciencias Sociales y Humana. CINDE, 2003 El presente trabajo de investigación Influencia del uso de mapas conceptuales en la construcción de la habilidad clasificación en ciencias naturales, parte de la consideración de que la dinámica del mundo contemporáneo y globalizado, exige la formación de ciudadanos con capacidad para comprender los cambios que se producen en la sociedad y para enfrentar un mu...

Influencia de los fact ores socioeconómicos en la deserción estudiantil de la Carrera de Ciencias Sociales

OpenAIRE

Dominga del Socorro Sevilla Núñez; Víctor Adrián Puerta Chavarría; Jacoba Dávila Molina

2012-01-01

El propósito fue identificar y determinar la influencia de los factores socioeconómicos en la deserción estudiantil de la carrera Ciencias Sociales con Mención en Desarrollo Local, 2006 – 2007, URACCAN, Recinto Las Minas, y sugerir estrategias que contribuyan a disminuir la permanencia del fenómeno en la carrera.La metodología se ubicó en el paradigma cualitativo con un enfoque teóricometodológico del interaccionismo simbólico. Se realizaron entrevistas individuales y grupales, además de la o...

Influencia de la composición del medio de cultivo sobre la producción de pigmento por Streptococcus agalactiae

OpenAIRE

Carazo Carazo, Concepción

2011-01-01

Se ha estudiado la influencia de la composición del medio de cultivo sobre la producción de pigmento por s. agalactiae partiendo de un medio de cultivo diseñado para tal finalidad (new granada medium), eliminando uno a uno los componentes incluidos en su formulación original. Almidón, proteosa peptona (difco), suero, fosfatos y metrotexato influyen de forma importante en la formación y/o visualización del pigmento producido por este microorganismo. Se ha determinado que la fracción de proteos...

La bailoterapia y su influencia en el estilo de vida de los pacientes del Centro Geriátrico Nueva Esperanza

OpenAIRE

Barros Pilay, Carla Rosalía

2011-01-01

El presente proyecto está enmarcado en un estudio analítico acerca de la Bailoterapia y su Influencia en el estilo de vida de los pacientes del Centro Geriátrico Nueva Esperanza, del Cantón Cuenca, Provincia Azuay, cumpliendo con el objetivo general: contribuir al mejoramiento de la salud y calidad de vida de las personas que reciben atención integral en el Centro Geriátrico Nueva Esperanza, manejando adecuadamente la bailoterapia, creando y brindando hábitos en el ámbito físico y recreativo,...

Influencia española en la enfermería colombiana Spanish influence Colombian nursing

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Ana Luisa Velandia M

1993-07-01

Full Text Available La autora establece cuatro herencias recibidas por la enfermería colombiana: la tradición de tipo religioso, la condición femenina o de género, la herencia militar y la herencia antropológica o étnica. En esta última se tiene en cuenta tanto la herencia indígena como la influencia de la enfermería española, con todos sus influjos mediterráneos. La influencia religiosa se inicia con el sacerdote Jesuita Pedro Claver hacia 1610 -1617; continúa con los Hermanos Hospitalarios de San Juan de Dios hacia 1768 y posteriormente en 1873 con la llegada de las Hermanas de la Caridad de la Presentación. Compara la organización de la enfermería en el Hospital de la Santa Cruz de Barcelona, donde parece ser se empezó a organizar la enfermería como tal, analizando las funciones del Administrador de la sala de enfermos, el Servidor, las mujeres encargadas de las salas de enfermas o de niños, la Ecónoma, la Jefe de Sala, y el Enfermero Mayor, en relación con los cargos y funciones actuales en los Hospitales colombianos.Four statements inferenced from: religious traditions, gender or woman status, military heritage and ethnic inheritance, and their influence in nursing, are presented in this article. The ethnic inheritance analyses the issue based upon the cultural influences of the native-indigenous groups and the Spanish and Mediterranean attributes in nursing development.The religious tradition began with Pedro Claver's J.C. presence between 1610 and 1617. His presence is followed by the "Hermanos Hospilalarios de San Juan de Dios" in 1768, and further with the presence of the sisters of Charity in 1873. Lastly, the article compares the Barcelona's Santa Cruz hospital organization, where at that time, seems to appear a new type of nursing arrangement; which present functions and charges, currently utilized in Colombian hospitals ( administrator of patient rooms, "Servidor", women in charge of female patients and sick children, "economa

Influencia de la lactancia materna en la formación del vínculo y en el desarrollo psicomotor

OpenAIRE

Marín Ospina, Jessica; Jiménez, Ángela María; Villamarín Betancourt, Eder

2016-01-01

La lactancia materna es un acto singular que tiene un valor importante para la madre y el lactante en lo biológico, lo psicológico y lo social. Esta investigación pretende describir la influencia que tiene la lactancia materna en la formación del vínculo afectivo y en el desarrollo psicomotor en niños amamantados y no amamantados de 6 a 8 meses de edad del hospital Mario Correa Rengifo de la ciudad de Cali, a partir de una investigación cualitativa, descriptiva, de tipo estudio de casos múlt...

Influencia de la música sobre los prerrequisitos del lenguaje en niños con Síndrome de Down

OpenAIRE

Moreno Fernández, Lidia

2015-01-01

El Síndrome de Down es un trastorno genético producido por la presencia de un cromosoma extra en el par 21, de tal forma que las células de estas personas tienen 47 cromosomas. Esta alteración genética presenta unas manifestaciones clínicas características, entre las que se encuentra la dificultad en el desarrollo del lenguaje. El objetivo es demostrar la influencia de la música sobre los prerrequisitos del lenguaje en niños con Síndrome de Down. Esto se llevó a cabo con el diseño y la puesta...

INFLUENCIA DE TÉCNICAS DE MEJORA DE SUELOS SOBRE LA FUNCIÓN HÍDRICA DE PASTOS NATURALES ALTOANDINOS.

OpenAIRE

Oscanoa, Luis; Ph.D. (c) Investigador asociado, The Mountain Institute – Laboratorio Pastizales UNALM, Lima Perú.; Flores, Enrique; Ph.D. Manejo de Pastizales, Profesor Principal EPG UNALM, Lima Perú.

2016-01-01

Se investigaron los pastos naturales degradados de la comunidad campesina Cordillera Blanca, Huaraz – Perú. El objetivo fue estudiar la influencia de las técnicas de mejora (T) sobre el estado ecológico (Ee), recuperación hidrológica (H) y control de la erosión (Er) del pastizal de condición muy pobre. El diseño experimental fue completamente al azar con arreglo factorial (3 técnicas x 6 épocas por parámetro de estudio). La investigación duró dos años, etapa en la cual se estudiaron el Ee (ve...

Morteros aligerados con arcilla expandida: influencia de la granulometría y la adicción de fibras de vidrio ar en el comportamiento mecánico

Directory of Open Access Journals (Sweden)

González Cortina, Mariano

2005-06-01

Full Text Available The study of lightweight cement mortars containing expanded clay aggregate described in this article was conducted to determine the effect of adding fibreglass fibres able to resist the alkalis in cement on the mechanical performance of the mortar. This involved analyzing the following variables: fibre length, degree and effect of dispersion and clay particle size distribution. The lightweight mortars studied are very low density mortars primarily used as fill in different construction situations.En este artículo se presenta un estudio realizado sobre morteros de cemento aligerados con arcilla expandida, para determinar la influencia de la adición de fibras de vidrio resistentes a los álcalis del cemento (AR, en el análisis de las variables: longitud de la fibra, porcentaje e influencia de la dispersabilidad, y la granulometría de la arcilla, sobre el comportamiento mecánico de los mismos. Los morteros aligerados analizados son morteros de muy baja densidad que se están utilizando como material de relleno, fundamentalmente, en distintas soluciones constructivas.

INFLUENCIA DE LA LATERALIDAD EN LA ADQUISICIÓN DE LA ESCRITURA EN UNA MUESTRA DE 27 ALUMNOS DE 3º PRIMARIA

OpenAIRE

Cañamero-de-León, Ana

2013-01-01

El objetivo principal de este trabajo es ver la influencia que puede ejercer la lateralidad sobre la adquisición de la escritura. Comprobar si realmente la lateralidad afecta significativamente a la hora de aprender a escribir correctamente. El estudio se ha llevado a cabo en un centro concertado de Madrid capital. Los alumnos que han participado en el presente estudio pertenecen a una clase de 3º de Primaria. Para ello se han medido ambas variables (lateralidad y escritura), y se ha lleva...

Valoración de la influencia de la práctica del fútbol en la evolución de la fuerza, la flexibilidad y la velocidad en población infantil

Directory of Open Access Journals (Sweden)

Silvia Sedano Campo*

2007-03-01

Full Text Available El objetivo del estudio es valorar la influencia que la práctica regular del fútbol tiene sobre algunos aspectos de la condición física (fuerza explosiva del tren inferior, flexibilidad y velocidad en niños de entre 7 y 14 años. Por otro lado también pretendemos determinar la correlación existente entre las tres capacidades evaluadas, comparar los resultados con población infantil general y analizar las posibles influencias que la práctica de este deporte puede tener en el desarrollo evolutivo. La muestra se compone de 106 niños que practican fútbol federado en clubes de la comunidad de Castilla y León. En primer lugar determinamos el peso y la talla de cada participante en el estudio y posteriormente pasamos tres pruebas de valoración de la condición física que forman parte de la batería EUROFIT: Flexión de tronco desde posición de sentados, salto horizontal y carrera de 10 X 5 m ida y vuelta. Se observa la importante influencia que la práctica del fútbol tiene en la flexibilidad y la velocidad cuando comparamos los datos con los obtenidos en escolares de la misma comunidad autónoma. Se observa también la correlación fuerte y positiva existente entre la fuerza y la velocidad

Consumo y percepción juvenil sobre la ficción seriada televisiva: influencia por sexo y edad (Young adult consumption and perception of TV fiction: sex –and age– based influence

Directory of Open Access Journals (Sweden)

María Isabel Menéndez Menéndez

2017-12-01

Full Text Available Este artículo se centra en la influencia de la ficción seriada televisiva en la construcción de la identidad juvenil. Los objetivos del trabajo son conocer la dieta mediática de la juventud española sobre ficción seriada, así como la percepción que tiene sobre la influencia de dichos formatos en sus vidas. Las principales conclusiones revelan el todavía gran consumo de series de ficción televisiva entre jóvenes en nuestro país, aunque se aprecian diferencias significativas entre los gustos masculinos y femeninos y entre las distintas edades, además de la mayor percepción de influencia entre las chicas en todos los ítems cuestionados. | This paper focuses on the influence of fictional TV series on the identity construction of young adults. The aims of the project are to acquire knowledge about the youth’s habits of consumption of TV series and to learn about their perception of the influence of this type of product on their lives. Our main conclusions reveal that there still exists an important habit of consumption of TV series among the youth in our country, although significant variations can be appreciated in the preferences of male and female informants of different age groups. To this we can add that girls have a stronger perception of the influence of all the items in the questionnaire.

Photoinduced Bioorthogonal 1,3-Dipolar Poly-cycloaddition Promoted by Oxyanionic Substrates for Spatiotemporal Operation of Molecular Glues.

Science.gov (United States)

Hatano, Junichi; Okuro, Kou; Aida, Takuzo

2016-01-04

PGlue(PZ), a pyrazoline (PZ)-based fluorescent adhesive which can be generated spatiotemporally in living systems, was developed. Since PGlue(PZ) carries many guanidinium ion (Gu(+)) pendants, it strongly adheres to various oxyanionic substrates through a multivalent salt-bridge interaction. PGlue(PZ) is given by bioorthogonal photopolymerization of a Gu(+)-appended monomer (Glue(TZ)), bearing tetrazole (TZ) and olefinic termini. Upon exposure to UV light, Glue(TZ) transforms into a nitrileimine (NI) intermediate (Glue(NI)), which is eligible for 1,3-dipolar polycycloaddition. However, Glue(NI) in aqueous media can concomitantly be deactivated into Glue(WA) by the addition of water, and the polymerization hardly occurs unless Glue(NI) is concentrated. We found that, even under high dilution, Glue(NI) is concentrated on oxyanionic substrates to a sufficient level for the polymerization, so that their surfaces can be point-specifically functionalized with PGlue(PZ) by the use of a focused beam of UV light. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Effects of the dual TP receptor antagonist and thromboxane synthase inhibitor EV-077 on human endothelial and vascular smooth muscle cells

International Nuclear Information System (INIS)

Petri, Marcelo H.; Tellier, Céline; Michiels, Carine; Ellertsen, Ingvill; Dogné, Jean-Michel; Bäck, Magnus

2013-01-01

Highlights: •EV-077 reduced TNF-α induced inflammation in endothelial cells. •The thromboxane mimetic U69915 enhanced vascular smooth muscle cell proliferation. •EV-077 inhibited smooth muscle cell proliferation. -- Abstract: The prothrombotic mediator thromboxane A 2 is derived from arachidonic acid metabolism through the cyclooxygenase and thromboxane synthase pathways, and transduces its effect through the thromboxane prostanoid (TP) receptor. The aim of this study was to determine the effect of the TP receptor antagonist and thromboxane synthase inhibitor EV-077 on inflammatory markers in human umbilical vein endothelial cells and on human coronary artery smooth muscle cell proliferation. To this end, mRNA levels of different proinflammatory mediators were studied by real time quantitative PCR, supernatants were analyzed by enzyme immune assay, and cell proliferation was assessed using WST-1. EV-077 significantly decreased mRNA levels of ICAM-1 and PTX3 after TNFα incubation, whereas concentrations of 6-keto PGF1α in supernatants of endothelial cells incubated with TNFα were significantly increased after EV-077 treatment. Although U46619 did not alter coronary artery smooth muscle cell proliferation, this thromboxane mimetic enhanced the proliferation induced by serum, insulin and growth factors, which was significantly inhibited by EV-077. In conclusion, EV-077 inhibited TNFα-induced endothelial inflammation and reduced the enhancement of smooth muscle cell proliferation induced by a thromboxane mimetic, supporting that the thromboxane pathway may be associated with early atherosclerosis in terms of endothelial dysfunction and vascular hypertrophy

Effects of the dual TP receptor antagonist and thromboxane synthase inhibitor EV-077 on human endothelial and vascular smooth muscle cells

Energy Technology Data Exchange (ETDEWEB)

Petri, Marcelo H. [Department of Medicine, Karolinska Institutet and Center for Molecular Medicine, Karolinska University Hospital, Stockholm (Sweden); Tellier, Céline; Michiels, Carine [NARILIS, URBC, University of Namur, Namur (Belgium); Ellertsen, Ingvill [Department of Medicine, Karolinska Institutet and Center for Molecular Medicine, Karolinska University Hospital, Stockholm (Sweden); Dogné, Jean-Michel [Department of Pharmacy, Namur Thrombosis and Hemostasis Center, University of Namur, Namur (Belgium); Bäck, Magnus, E-mail: Magnus.Back@ki.se [Department of Medicine, Karolinska Institutet and Center for Molecular Medicine, Karolinska University Hospital, Stockholm (Sweden)

2013-11-15

Highlights: •EV-077 reduced TNF-α induced inflammation in endothelial cells. •The thromboxane mimetic U69915 enhanced vascular smooth muscle cell proliferation. •EV-077 inhibited smooth muscle cell proliferation. -- Abstract: The prothrombotic mediator thromboxane A{sub 2} is derived from arachidonic acid metabolism through the cyclooxygenase and thromboxane synthase pathways, and transduces its effect through the thromboxane prostanoid (TP) receptor. The aim of this study was to determine the effect of the TP receptor antagonist and thromboxane synthase inhibitor EV-077 on inflammatory markers in human umbilical vein endothelial cells and on human coronary artery smooth muscle cell proliferation. To this end, mRNA levels of different proinflammatory mediators were studied by real time quantitative PCR, supernatants were analyzed by enzyme immune assay, and cell proliferation was assessed using WST-1. EV-077 significantly decreased mRNA levels of ICAM-1 and PTX3 after TNFα incubation, whereas concentrations of 6-keto PGF1α in supernatants of endothelial cells incubated with TNFα were significantly increased after EV-077 treatment. Although U46619 did not alter coronary artery smooth muscle cell proliferation, this thromboxane mimetic enhanced the proliferation induced by serum, insulin and growth factors, which was significantly inhibited by EV-077. In conclusion, EV-077 inhibited TNFα-induced endothelial inflammation and reduced the enhancement of smooth muscle cell proliferation induced by a thromboxane mimetic, supporting that the thromboxane pathway may be associated with early atherosclerosis in terms of endothelial dysfunction and vascular hypertrophy.

Advanced Amine Solvent Formulations and Process Integration for Near-Term CO2 Capture Success

Energy Technology Data Exchange (ETDEWEB)

Fisher, Kevin S.; Searcy, Katherine; Rochelle, Gary T.; Ziaii, Sepideh; Schubert, Craig

2007-06-28

This Phase I SBIR project investigated the economic and technical feasibility of advanced amine scrubbing systems for post-combustion CO2 capture at coal-fired power plants. Numerous combinations of advanced solvent formulations and process configurations were screened for energy requirements, and three cases were selected for detailed analysis: a monoethanolamine (MEA) base case and two “advanced” cases: an MEA/Piperazine (PZ) case, and a methyldiethanolamine (MDEA) / PZ case. The MEA/PZ and MDEA/PZ cases employed an advanced “double matrix” stripper configuration. The basis for calculations was a model plant with a gross capacity of 500 MWe. Results indicated that CO2 capture increased the base cost of electricity from 5 cents/kWh to 10.7 c/kWh for the MEA base case, 10.1 c/kWh for the MEA / PZ double matrix, and 9.7 c/kWh for the MDEA / PZ double matrix. The corresponding cost per metric tonne CO2 avoided was 67.20 $/tonne CO2, 60.19 $/tonne CO2, and 55.05 $/tonne CO2, respectively. Derated capacities, including base plant auxiliary load of 29 MWe, were 339 MWe for the base case, 356 MWe for the MEA/PZ double matrix, and 378 MWe for the MDEA / PZ double matrix. When compared to the base case, systems employing advanced solvent formulations and process configurations were estimated to reduce reboiler steam requirements by 20 to 44%, to reduce derating due to CO2 capture by 13 to 30%, and to reduce the cost of CO2 avoided by 10 to 18%. These results demonstrate the potential for significant improvements in the overall economics of CO2 capture via advanced solvent formulations and process configurations.

Efficacy of Polaprezinc for Acute Radiation Proctitis in a Rat Model

International Nuclear Information System (INIS)

Doi, Hiroshi; Kamikonya, Norihiko; Takada, Yasuhiro; Fujiwara, Masayuki; Tsuboi, Keita; Inoue, Hiroyuki; Tanooka, Masao; Nakamura, Takeshi; Shikata, Toshiyuki; Tsujimura, Tohru; Hirota, Shozo

2011-01-01

Purpose: The purpose of the present study was to standardize the experimental rat model of radiation proctitis and to examine the efficacy of polaprezinc on radiation proctitis. Methods and Materials: A total of 54 female Wistar rats (5 weeks old) were used. The rats were divided into three groups: those treated with polaprezinc (PZ+), those treated with base alone, exclusive of polaprezinc (PZ-), and those treated without any medication (control). All the rats were irradiated to the rectum. Polaprezinc was prepared as an ointment. The ointment was administered rectally each day after irradiation. All rats were killed on the 10th day after irradiation. The mucosal changes were evaluated endoscopically and pathologically. The results were graded from 0 to 4 and compared according to milder or more severe status, as applicable. Results: According to the endoscopic findings, the proportion of mild changes in the PZ+, PZ-, and control group was 71.4%, 25.0%, and 14.3% respectively. On pathologic examination, the proportion of low-grade findings in the PZ+, PZ-, and control group was 80.0%, 58.3%, and 42.9% for mucosal damage, 85.0%, 41.7%, and 42.9% for a mild degree of inflammation, and 50.0%, 33.3%, and 4.8% for a shallow depth of inflammation, respectively. The PZ+ group tended to have milder mucosal damage than the other groups, according to all criteria used. In addition, significant differences were observed between the PZ+ and control groups regarding the endoscopic findings, degree of inflammation, and depth of inflammation. Conclusions: This model was confirmed to be a useful experimental rat model for radiation proctitis. The results of the present study have demonstrated the efficacy of polaprezinc against acute radiation-induced rectal disorders using the rat model.

Análisis competitivo mediante grupos estratégicos y su influencia sobre el desempeño: aplicación al sector hotelero español

OpenAIRE

Pereira-Moliner, Jorge

2005-01-01

Este documento de trabajo pretende reflejar, de forma resumida, los resultados obtenidos en la tesis titulada “Análisis competitivo mediante grupos estratégicos y su influencia sobre el desempeño: aplicación al sector hotelero español” que fue presentada en el Departamento de Organización de Empresas de la Universidad de Alicante en 2005.

Las abejas de Porce familia colletidae (hymenoptera: apoidea notas y claves para los géneros presentes en la zona de influencia del embalse Porce ii.

Directory of Open Access Journals (Sweden)

Smith Pardo Allan H.

1999-12-01

Full Text Available Se presentan claves taxonómicas a nivel de género para la identificación de las abejas colletidas de la zona de Influencia del Embalse Porce II (Porce, Antioquía, Colombia S.A. Adicionalmente se presentan algunas notas para los géneros de colletidos capturados durante el inventario de las abejas silvestres de la zona.

Modelling of bubbly and annular two-phase flow in subchannel geometries with BACCHUS-3D/TP

International Nuclear Information System (INIS)

Bottoni, M.; Lyczkowski, R.W.

1992-01-01

The theoretical and computational bases of the BACCHUS-3D/TP computer program are reviewed. The computer program is used for thermal-hydraulic analyses of nuclear fuel bundles under normal and accident conditions. The present program combines two models and solution procedures previously used separately, namely, the Improved Slip Model (ISM) and the Separated-Phases Model (SPM). The former model uses mixture equations with accounting for slip between the phases, whereas the latter uses separate continuity and momentum equations. At the present stage of development, both assume thermodynamic equilibrium. Techniques used to affect smooth transition between the two models are described. including treatment of frictional pressure drop and solution of the Poisson and momentum equations. A detailed derivation of the computation of mass transfer between the phases is given because it is a central and novel feature of the model

Track treeing mechanism and its application

International Nuclear Information System (INIS)

Li Boyang

1993-01-01

Based on electrostriction and fracture mechanics, experiment observation and data-processing, two models (restriction among tree tracks and induction of tree track onto stress concentrated spots) and factors (restriction and induction) are proposed; The existence of four types of plastic zone (spot-block pz, single crack isolate pz, transition from isolate to block pz and crack-block pz) and two types of annex (plastic zone and crack zone) are pointed out. The development regularities of Gp (diameter of plastic zone), G(diameter of tree track), S(tree track density) and total areal of tree track (SG 2 ) in two basic experiments (H=H+dH, H=Hc H-field strength) are described by using four basic formulae. (author)

El fantasma del abstencionismo y la influencia mediática

Directory of Open Access Journals (Sweden)

Lic. Edith Barajas

2001-01-01

Full Text Available Nadie duda, en el inicio de un nuevo milenio, de la influencia y el impacto de los medios masivos de comunicación. La prensa, la radio, la televisión y, contemporáneamente Internet, juegan un papel clave en la nueva era de la humanidad, donde la paradoja es tener información a mansalva para un ser actual dominado por fenómenos como el cortoplacismo, los antimodelos, las guerras, el esoterismo, la hambruna, la religión y la violencia, todo englobado, a su vez, en una economía mundializada que rompe y descarna las fronteras de las naciones, y en donde los futurólogos citan las dos grandes vías: la era digital y la era biológica.En el llamado "control de masas", el medio masivo, especialmente el electrónico, desarrolla un actor importante dentro del mundo global, pues mantiene a la mayoría de la población concentrada en temas que no son precisamente los del orden de mayor interés (en nuestro país, podemos considerar el escándalo Trevi-Andrade como un elemento distractor, sobre los problemas que aquejan al país; es decir, la opinión pública se concentra en ese enfoque, antes de irse a lo medular

T-p phase diagrams and the barocaloric effect in materials with successive phase transitions

Science.gov (United States)

Gorev, M. V.; Bogdanov, E. V.; Flerov, I. N.

2017-09-01

An analysis of the extensive and intensive barocaloric effect (BCE) at successive structural phase transitions in some complex fluorides and oxyfluorides was performed. The high sensitivity of these compounds to a change in the chemical pressure allows one to vary the succession and parameters of the transformations (temperature, entropy, baric coefficient) over a wide range and obtain optimal values of the BCE. A comparison of different types of schematic T-p phase diagrams with the complicated T( p) dependences observed experimentally shows that in some ranges of temperature and pressure the BCE in compounds undergoing successive transformations can be increased due to a summation of caloric effects associated with distinct phase transitions. The maximum values of the extensive and intensive BCE in complex fluorides and oxyfluorides can be realized at rather low pressure (0.1-0.3 GPa). In a narrow temperature range around the triple points conversion from conventional BCE to inverse BCE is observed, which is followed by a gigantic change of both \\vertΔ S_BCE\\vert and \\vertΔ T_AD\\vert .

Influencia de las Emociones en el Proceso de Aprendizaje en Educación Inicial en Niños de 4 a 5 Años de Edad en el Centro de Estimulación Integral y Apoyo Psicoterapéutico de la Universidad del Azuay "CEIAP"

OpenAIRE

Zamora Torres, Johanna Elizabeth

2013-01-01

La presente investigación aborda la influencia de las emociones en el proceso de aprendizaje en educación inicial en niños de 4 a 5 años de edad, considerando que en esta etapa se desarrollan las diferentes áreas: cognitiva, motriz, lenguaje, social, afectiva, y el aspecto emocional indispensable para el correcto desenvolvimiento futuro del niño. Se abordará sobre la influencia de las emociones en el proceso enseñanza-aprendizaje ya que tradicionalmente ha existido una separación casi ab...

La gestión administrativa y su influencia en el comportamiento humano de la empresa mercado de productores de Santa Anita

OpenAIRE

Colan Aranda, Hugo Humberto

2014-01-01

El presente trabajo de investigación titulado "LA GESTIÓN ADMINISTRATIVA Y SU INFLUENCIA EN EL COMPORTAMIENTO HUMANO DE LA EMPRESA MERCADO DE PRODUCTORES DE SANTA ANITA" se realizó con la finalidad de determinar si la Gestión Administrativa, el planeamiento estratégico, la visión, la misión, los objetivos, los reglamentos, normas directivas influyen y son necesarias para brindar un mejor Comportamiento Humano en el Mercado de Productores de Santa Anita. El diseño metodológico de la investigac...

Análisis CFD sobre la influencia de la solidez en aerogeneradores H-Darrieus con perfiles asimétricos NACA 7425

OpenAIRE

Rivero Sánchez, Alonso

2016-01-01

El presente trabajo de fin de grado es un estudio CFD de un aerogenerador de eje vertical tipo H-Darrieus de pequeñas dimensiones, compuesto por tres álabes con perfiles asimétricos NACA 7425. El objetivo principal es analizar la influencia de la solidez en un aerogenerador con estas características, para ello se estudiaran diferentes modelos posibles, rotor completo y perfiles estáticos. En la práctica se han realizado diversas simulaciones para llegar a obtener las curvas características de...

Estudio de la influencia de la inteligencia y el género en la evaluación matemática temprana

OpenAIRE

Estíbaliz L. Aragón; Cándida I. Delgado; Manuel Aguilar; Antonio Araújo; José I. Navarro

2013-01-01

En este estudio se trató de detectar el alumnado en riesgo de presentar dificultades de aprendizaje de las matemáticas, en los niveles iniciales de la escolarización obligatoria, satisfaciendo la necesidad de llevar a cabo una evaluación completa y exhaustiva, determinando qué aspectos de la evaluación del rendimiento matemático correlacionan con la medida de la inteligencia. Asimismo, se analizaron las diferencias de género en matemática temprana, revelando la posible influencia del género e...

Influencia del liderazgo y de las tecnologías de la información y la comunicación en la percepción de seguridad ciudadana de las juntas vecinales de la Comisaría de Pro – Los Olivos, 2016

OpenAIRE

Canduelas Sabrera, Francisco Regart

2017-01-01

La presente investigación titulada “Influencia del liderazgo y de las tecnologías de la información y la comunicación en la percepción de seguridad ciudadana de las juntas vecinales de la comisaría de Pro – Los Olivos, 201 6”, tuvo como objetivo general determinar la influencia existente entre las variables liderazgo y tecnologías de la información y la comunicación en la percepción de seguridad ciudadana. La investigación fue transeccional relacional-causal. Para la recolec...

Abordajes teóricos en la comprensión de la influencia de los recursos económicos en la actividad física

OpenAIRE

Montenegro Mendivelso, Mónica

2015-01-01

Estudio cualitativo que analiza los abordajes teóricos utilizados por diferentes autores en la comprensión de la influencia de los recursos económicos en la actividad física desde los modelos de determinantes y determinación social. Qualitative study that analyzes the theoretical approaches used by different authors in the understanding of the influence of economic resources in the physical activity, from determinants model and social determination model.

Zone-specific logistic regression models improve classification of prostate cancer on multi-parametric MRI

Energy Technology Data Exchange (ETDEWEB)

Dikaios, Nikolaos; Halligan, Steve; Taylor, Stuart; Atkinson, David; Punwani, Shonit [University College London, Centre for Medical Imaging, London (United Kingdom); University College London Hospital, Departments of Radiology, London (United Kingdom); Alkalbani, Jokha; Sidhu, Harbir Singh [University College London, Centre for Medical Imaging, London (United Kingdom); Abd-Alazeez, Mohamed; Ahmed, Hashim U.; Emberton, Mark [University College London, Research Department of Urology, Division of Surgery and Interventional Science, London (United Kingdom); Kirkham, Alex [University College London Hospital, Departments of Radiology, London (United Kingdom); Freeman, Alex [University College London Hospital, Department of Histopathology, London (United Kingdom)

2015-09-15

To assess the interchangeability of zone-specific (peripheral-zone (PZ) and transition-zone (TZ)) multiparametric-MRI (mp-MRI) logistic-regression (LR) models for classification of prostate cancer. Two hundred and thirty-one patients (70 TZ training-cohort; 76 PZ training-cohort; 85 TZ temporal validation-cohort) underwent mp-MRI and transperineal-template-prostate-mapping biopsy. PZ and TZ uni/multi-variate mp-MRI LR-models for classification of significant cancer (any cancer-core-length (CCL) with Gleason > 3 + 3 or any grade with CCL ≥ 4 mm) were derived from the respective cohorts and validated within the same zone by leave-one-out analysis. Inter-zonal performance was tested by applying TZ models to the PZ training-cohort and vice-versa. Classification performance of TZ models for TZ cancer was further assessed in the TZ validation-cohort. ROC area-under-curve (ROC-AUC) analysis was used to compare models. The univariate parameters with the best classification performance were the normalised T2 signal (T2nSI) within the TZ (ROC-AUC = 0.77) and normalized early contrast-enhanced T1 signal (DCE-nSI) within the PZ (ROC-AUC = 0.79). Performance was not significantly improved by bi-variate/tri-variate modelling. PZ models that contained DCE-nSI performed poorly in classification of TZ cancer. The TZ model based solely on maximum-enhancement poorly classified PZ cancer. LR-models dependent on DCE-MRI parameters alone are not interchangeable between prostatic zones; however, models based exclusively on T2 and/or ADC are more robust for inter-zonal application. (orig.)