WorldWideScience

Sample records for testis osalesid hp

  1. Hobusepea ja Rael Arteli galerii osalesid möödunud nädalavahetusel... / Elin Kard ; interv. Reet Varblane

    Index Scriptorium Estoniae

    Kard, Elin

    2008-01-01

    Galeriid osalesid Berliini Kunstiakadeemias IGBK (Internationale Gesellschaft der Bildenden Künste ehk Visuaalkunsti Rahvusvahelise Ühingu) korraldatud non-profit projektis "Art Swap Europe/Euroopa kunstivahetus". Projekti eesmärgist, mida see andis Hobusepea galeriile ja annab kunstnikele

  2. HP 9816

    CERN Multimedia

    1982-01-01

    The 9816 was introduced in late 1982. This was the low-cost model in the 200 Series range. It only had two expansion slots and featured a monitor integrated with the system unit and modular keyboard and mass storage (usually a 9121 dual 3.5 inch floppy drive). The monitor was nine inches diagonally with a 400 by 300 dot resolution. The HP 9816 was also designated as the HP 9000 216. It did not include any disk drives but it had a built-in 9 inch monochrome monitor, built-in HP-IB and RS-232 ports and 2 expansion slots. The standard keyboard for the 9816 is a itty-bitty number. The 9816 A came with 128K bytes of memory. The 9816 S included all of the above plus disk based BASIC and a card containing an additional 256K of memory bringing the total memory to 512K but only leaving only one expansion slot open.

  3. 3HP tolerance

    DEFF Research Database (Denmark)

    2014-01-01

    Cells and cell cultures are provided that have improved tolerance to 3-hydroxypropionic acid (3HP). Genetic modifications to provide a mutated or overexpressed SFA1 gene or other enhancement of 3HP detoxification via a glutathione- dependent dehydrogenase reaction, including medium supplementation...

  4. HP upgrade operational streamlining

    Science.gov (United States)

    Edge, David R.; Emenheiser, Kenneth S.; Hanrahan, William P., III; Mccollums, D.; Seery, Paul J.; Ricklefs, Randall L.

    1993-01-01

    New computer technology and resources must be successfully integrated into CDSLR station operations to manage new complex operational tracking requirements, support the on site production of new data products, support ongoing station performance improvements, and to support new station communication requirements. The NASA CDSLR Network is in the process of upgrading station computer resources with HP UNIX workstations, designed to automate a wide range of operational station requirements. The primary HP upgrade objective was to relocate computer intensive data system tasks from the controller computer to a new advanced computer environment designed to meet the new data system requirements. The HP UNIX environment supports fully automated real time data communications, data management, data processing, and data quality control. Automated data compression procedures are used to improve the efficiency of station data communications. In addition, the UNIX environment supports a number of semi-automated technical and administrative operational station tasks. The x window user interface generates multiple simultaneous color graphics displays, providing direct operator visibility and control over a wide range of operational station functions.

  5. Building the mammalian testis

    DEFF Research Database (Denmark)

    Svingen, Terje; Koopman, Peter

    2013-01-01

    Development of testes in the mammalian embryo requires the formation and assembly of several cell types that allow these organs to achieve their roles in male reproduction and endocrine regulation. Testis development is unusual in that several cell types such as Sertoli, Leydig, and spermatogonial...

  6. The undescended testis

    DEFF Research Database (Denmark)

    Vikraman, Jaya; Hutson, John M; Li, Ruili

    2016-01-01

    Undescended testes (UDT), where one or both testes fail to migrate to the base of the scrotum, can be congenital (2-5% of newborn males) or acquired (1-2% of males). The testis may be found in any position along its usual line of descent. Cryptorchidism affects the developing testicular germ cells...... and increases the risk of infertility and malignancy. Clinical management aims to preserve spermatogenesis and prevent the increased risk of seminoma. Examination to document the testicular position will guide the need for imaging, medical management and the surgical approach to orchidopexy....

  7. Descent of the testis

    DEFF Research Database (Denmark)

    Hutson, John M.; Thorup, Jørgen M.; Beasley, Spencer W.

    changes in the management of both congenital and acquired cryptorchidism, such as the widespread use of laparoscopy for impalpable testes inside the abdominal cavity, are fully described. Evidence for the benefits of orchidopexy within the first year of life is reviewed, and an individual chapter is also......This book provides a state of the art overview of all aspects of testicular descent and cryptorchidism, including the mechanisms of descent and the causes, consequences, diagnosis, and treatment of undescended testis. The advances in understanding that have been achieved over the past two decades...

  8. Perineal Ektopik Testis: Nadir Bir Konjenital Anomali

    OpenAIRE

    Çiçek, Tufan; Gönülalan, Umut; Öztürk, Bülent; Koşan, Tevfik Murat

    2013-01-01

    Perineal ectopic testis is a rare congenital anomally. The incidence of perineal ectopic testis is less than 1% in all undescended testis. Patient usually applied to urology with empty scrotum and testicular agenesia. The main treatment is surgical and hormonal therapy is not indicated in these patients. We report an eleven years old patient with right perineal ectopic testis that was underwent scrotal orchidopexy

  9. Phylogenomic analysis reveals dynamic evolutionary history of the Drosophila heterochromatin protein 1 (HP1 gene family.

    Directory of Open Access Journals (Sweden)

    Mia T Levine

    Full Text Available Heterochromatin is the gene-poor, satellite-rich eukaryotic genome compartment that supports many essential cellular processes. The functional diversity of proteins that bind and often epigenetically define heterochromatic DNA sequence reflects the diverse functions supported by this enigmatic genome compartment. Moreover, heterogeneous signatures of selection at chromosomal proteins often mirror the heterogeneity of evolutionary forces that act on heterochromatic DNA. To identify new such surrogates for dissecting heterochromatin function and evolution, we conducted a comprehensive phylogenomic analysis of the Heterochromatin Protein 1 gene family across 40 million years of Drosophila evolution. Our study expands this gene family from 5 genes to at least 26 genes, including several uncharacterized genes in Drosophila melanogaster. The 21 newly defined HP1s introduce unprecedented structural diversity, lineage-restriction, and germline-biased expression patterns into the HP1 family. We find little evidence of positive selection at these HP1 genes in both population genetic and molecular evolution analyses. Instead, we find that dynamic evolution occurs via prolific gene gains and losses. Despite this dynamic gene turnover, the number of HP1 genes is relatively constant across species. We propose that karyotype evolution drives at least some HP1 gene turnover. For example, the loss of the male germline-restricted HP1E in the obscura group coincides with one episode of dramatic karyotypic evolution, including the gain of a neo-Y in this lineage. This expanded compendium of ovary- and testis-restricted HP1 genes revealed by our study, together with correlated gain/loss dynamics and chromosome fission/fusion events, will guide functional analyses of novel roles supported by germline chromatin.

  10. Perineal Ectopic Testis: A Rare Congenital Anomally

    Directory of Open Access Journals (Sweden)

    Tufan Cicek

    2013-02-01

    Full Text Available Perineal ectopic testis is a rare congenital anomally. The incidence of perineal ectopic testis is less than 1% in all undescended testis. Patient usually applied to urology with empty scrotum and testicular agenesia. The main treatment is surgical and hormonal therapy is not indicated in these patients. We report an eleven years old patient with right perineal ectopic testis that was underwent scrotal orchidopexy [Cukurova Med J 2013; 38(1.000: 138-141

  11. Perineal Ectopic Testis: A Rare Congenital Anomally

    OpenAIRE

    Tufan Cicek; Umut Gonulalan; Bulent Ozturk; Tevfik Murat Kosan

    2013-01-01

    Perineal ectopic testis is a rare congenital anomally. The incidence of perineal ectopic testis is less than 1% in all undescended testis. Patient usually applied to urology with empty scrotum and testicular agenesia. The main treatment is surgical and hormonal therapy is not indicated in these patients. We report an eleven years old patient with right perineal ectopic testis that was underwent scrotal orchidopexy [Cukurova Med J 2013; 38(1.000): 138-141

  12. Testis. Strategy for the management of seminoma testis

    International Nuclear Information System (INIS)

    Fetterly, J.C.M.

    1984-01-01

    Many years of experience demonstrate the marked radiocurability of seminoma testis with modest radiation dosage, and accordingly large volumes may be radiated without damage to surrounding normal structures. While several histological types of seminoma are described (classical, anaplastic, or spermatocytic) our treatment strategy is identical

  13. Risk factors for undescended testis.

    NARCIS (Netherlands)

    Brouwers, M.M.; Bruijne, L.M. de; Gier, R.P.E. de; Zielhuis, G.A.; Feitz, W.F.J.; Roeleveld, N.

    2012-01-01

    OBJECTIVE: To contribute to the understanding of the etiology of undescended testis (UDT), by exploring a wide range of potential risk factors in a case-referent study. PATIENTS AND METHODS: Cases and referents were recruited at five hospitals and included 200 boys with surgically corrected UDT and

  14. H.P. Hanssens nationale retorik

    DEFF Research Database (Denmark)

    Kock, Christian Erik J

    2015-01-01

    Hvordan var H.P. Hanssens retorik om danskheden og det nationale? Dette spørgsmål stiller professor i retorik Christian Kock, Københavns Universitet.......Hvordan var H.P. Hanssens retorik om danskheden og det nationale? Dette spørgsmål stiller professor i retorik Christian Kock, Københavns Universitet....

  15. HP investeerib tulevikku / Fredy-Edwin Esse

    Index Scriptorium Estoniae

    Esse, Fredy-Edwin

    2010-01-01

    Maailma suurima tehnoloogiafirma Hewlett-Packard (HP) tootearenduskulud kasvasid IV kvartalis 16%, 814 mln dollarini. Autor märgib, et HP aktsia on praegu väga hea saak, kuid mingit imehüpet aktsia hinnas ei tuleks kindlasti oodata

  16. Hp: an inflammatory indicator in cardiovascular disease.

    Science.gov (United States)

    Graves, Karen L; Vigerust, David J

    2016-07-01

    Over the past decade significant advancement has occurred in the biological and pathological role that Hp has in cardiovascular disease. Hp is an acute-phase protein with a role in the neutralization and clearance of free heme. Iron has tremendous potential for initiating vascular oxidation, inflammation and exacerbating coronary atherosclerosis. Hp genotype has been linked as a prognostic biomarker of acute myocardial infarction, heart failure, restenosis and cardiac transplant rejection. The increased understanding of Hp as a biomarker has provided new insights into the mechanisms of inflammation after cardiac injury and support the concept that Hp is not only an important antioxidant in vascular inflammation and atherosclerosis, but also an enhancer of inflammation in cardiac transplant.

  17. Effectiveness of testis cancer screening

    International Nuclear Information System (INIS)

    Feucht, H.

    1983-04-01

    In the Federal Republic of Germany there are about thousand to two-thousand incidences of testis cancer yearly. The screening (and examination) program currently used for the early detection of cancer includes the screening of malignant tumours of the testis. Since only males 45 years and older are invited to make use of the preventive measures, the most seriously affected age-group between twenty and forty is hereby not considered. The objective of this study is to find out which of the generally conceivable preventive measures could lead to an improvement of the present situation. The analysis shows that, for a diagnostic accuracy of 1 and a participation of 100%, the quantifyable cost of a yearly performed special screening is higher than the quantifyable cost savings achieved. A final judgement of other ways of execution is only possible when the diagnostic accuracy of suitable screening methods and the percentage of the participants of the groups of people concerned are known. (orig.)

  18. [Laparoscopy for the undescended testis].

    Science.gov (United States)

    Broto, J; Marhuenda, C; Gil-Vernet, J M; Asensio, M; Lloret, J; Boix Ochoa, J

    1997-07-01

    The authors show their experience in the use of the laparoscopic approach in 18 cases of unilateral exploratory anorchia. They highlight the advantages that this method offers in efficiency, speed, security and minimal aggression compared with conventional exploration of the inguinal channel in the search of the undetectable testis. In the 18 cases studied through the insertion of 5 mm lense in the umbilical area, it was established that a testicular hypoplasia was present in 8 cases. In another 5 cases testicular agenesia was diagnosed, and in the remaining 5, intraabdominal testis were found. In four of these testicles, a laparoscopic approach helped in their correct placement and also in the removal of the fifth in a patient with recurrent leukemia.

  19. Cancer testis antigen and immunotherapy

    Directory of Open Access Journals (Sweden)

    Krishnadas DK

    2013-04-01

    Full Text Available Deepa Kolaseri Krishnadas, Fanqi Bai, Kenneth G Lucas Department of Pediatrics, Division of Hematology/Oncology, University of Louisville, KY, USA Abstract: The identification of cancer testis (CT antigens has been an important advance in determining potential targets for cancer immunotherapy. Multiple previous studies have shown that CT antigen vaccines, using both peptides and dendritic cell vaccines, can elicit clinical and immunologic responses in several different tumors. This review details the expression of melanoma antigen family A, 1 (MAGE-A1, melanoma antigen family A, 3 (MAGE-A3, and New York esophageal squamous cell carcinoma-1 (NY-ESO-1 in various malignancies, and presents our current understanding of CT antigen based immunotherapy. Keywords: cancer testis antigens, immunotherapy, vaccine

  20. Epigenetic regulation of hibernation-associated HP-20 and HP-27 gene transcription in chipmunk liver.

    Science.gov (United States)

    Tsukamoto, Daisuke; Ito, Michihiko; Takamatsu, Nobuhiko

    2018-01-08

    The chipmunk hibernation-related proteins (HPs) HP-20 and HP-27 are components of a 140-kDa complex that dramatically decreases in the blood during hibernation. The HP-20 and HP-27 genes are expressed specifically in the liver and are downregulated in hibernating chipmunks. Hibernation-associated physiological changes are assumed to be under genetic control. Therefore, to elucidate the molecular mechanisms of hibernation, here we examined the mechanisms behind the altered HP-20 and HP-27 gene expression in nonhibernating versus hibernating chipmunks. Chromatin immunoprecipitation (ChIP) analyses revealed that histone H3 on the HP-20 and HP-27 gene promoters was highly acetylated at lysine (K) 9 and K14 and highly trimethylated at K4 in the liver of nonhibernating chipmunks, while these active histone modifications were nearly absent in hibernating chipmunks. Furthermore, histone acetyltransferases and a histone methyltransferase were associated with the HP-20 and HP-27 gene promoters primarily in nonhibernating chipmunks. Consistent with a previous finding that HNF-1 and USF can activate HP-20 and HP-27 gene transcription by binding to the proximal promoter region, ChIP-quantitative PCR (qPCR) analyses revealed that significantly less HNF-1 and USF were bound to these gene promoters in hibernating than in nonhibernating chipmunks. These findings collectively indicated that the hibernation-associated HP-20 and HP-27 gene expression is epigenetically regulated at the transcriptional level by the binding of HNF-1 and USF to their proximal promoters, and that histone modification has a key role in hibernation-associated transcriptional regulation. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

  1. Optimal design of PV and HP system

    DEFF Research Database (Denmark)

    Nepper-Rasmussen, Bjarke Christian; Rasmussen, Theis Bo

    2015-01-01

    electric energy demand of the HP to hours where excess PV power is present. The self-consumption of the PV energy affects the overall net present value (NPV). In this paper, a method which maximizes the NPV by finding the cost-optimal combination of PV, HP and BT sizes, is proposed. Results show......Methods of utilizing residential produced photovoltaic (PV) power by converting to thermal energy through heat pumps (HP) are present in literature, where thermal energy is dispersed as either heat or hot water at the instant moment of PV production. In this paper an alternative solution...

  2. HP-SEE User Forum 2012

    CERN Document Server

    Karaivanova, Aneta; Oulas, Anastasis; Liabotis, Ioannis; Stojiljkovic, Danica; Prnjat, Ognjen

    2014-01-01

    This book is a collection of carefully reviewed papers presented during the HP-SEE User Forum, the meeting of the High-Performance Computing Infrastructure for South East Europe’s (HP-SEE) Research Communities, held in October 17-19, 2012, in Belgrade, Serbia. HP-SEE aims at supporting and integrating regional HPC infrastructures; implementing solutions for HPC in the region; and making HPC resources available to research communities in SEE, region, which are working in a number of scientific fields with specific needs for massively parallel execution on powerful computing resources. HP-SEE brings together research communities and HPC operators from 14 different countries and enables them to share HPC facilities, software, tools, data and research results, thus fostering collaboration and strengthening the regional and national human network; the project specifically supports research groups in the areas of computational physics, computational chemistry and the life sciences. The contributions presented i...

  3. Haptoglobin genotyping of Vietnamese: global distribution of HP del, complete deletion allele of the HP gene.

    Science.gov (United States)

    Soejima, Mikiko; Agusa, Tetsuro; Iwata, Hisato; Fujihara, Junko; Kunito, Takashi; Takeshita, Haruo; Lan, Vi Thi Mai; Minh, Tu Binh; Takahashi, Shin; Trang, Pham Thi Kim; Viet, Pham Hung; Tanabe, Shinsuke; Koda, Yoshiro

    2015-01-01

    The haptoglobin (HP) gene deletion allele (HP(del)) is responsible for anhaptoglobinemia and a genetic risk factor for anaphylaxis reaction after transfusion due to production of the anti-HP antibody. The distribution of this allele has been explored by several groups including ours. Here, we studied the frequency of HP(del) in addition to the distribution of common HP genotypes in 293 Vietnamese. The HP(del) was encountered with the frequency of 0.020. The present result suggested that this deletion allele is restricted to East and Southeast Asians. Thus, this allele seems to be a potential ancestry informative marker for these populations. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  4. Operation of the HP2250 with the HP9000 series 200 using PASCAL 3.0

    Science.gov (United States)

    Perry, John; Stroud, C. W.

    1986-01-01

    A computer program has been written to provide an interface between the HP Series 200 desktop computers, operating under HP Standard Pascal 3.0, and the HP2250 Data Acquisition and Control System. Pascal 3.0 for the HP9000 desktop computer gives a number of procedures for handling bus communication at various levels. It is necessary, however, to reach the lowest possible level in Pascal to handle the bus protocols required by the HP2250. This makes programming extremely complex since these protocols are not documented. The program described solves those problems and allows the user to immediately program, simply and efficiently, any measurement and control language (MCL/50) application with a few procedure calls. The complete set of procedures is available on a 5 1/4 inch diskette from Cosmic. Included in this group of procedures is an Exerciser which allows the user to exercise his HP2250 interactively. The exerciser operates in a fashion similar to the Series 200 operating system programs, but is adapted to the requirements of the HP2250. The programs on the diskette and the user's manual assume the user is acquainted with both the MCL/50 programming language and HP Standard Pascal 3.0 for the HP series 200 desktop computers.

  5. Eesti filmitegijad osalesid Põhjamaade filmifestivalil / Maris Lillak

    Index Scriptorium Estoniae

    Lillak, Maris, 1970-

    2003-01-01

    Newcastle-Upon-Tyne'is lõppes ligi 2 nädalat kestnud Põhjamaade filmifestival Nordic Lights, kus kesksel kohal näidati Tyne'i ja Tallinna vahelise lühifilmivõistluse tulemusi. Põhiprogrammis olid ka Arvo Iho "Karu süda" ja Ilkka Järvi-Laturi "Tallinn pimeduses". Oli ka ülevaatlik programm Eesti animatsioonist

  6. Lootuse festivalil osalesid lauljad Raplamaalt / Maire Kõrver

    Index Scriptorium Estoniae

    Kõrver, Maire, 1961-

    2009-01-01

    Tallinnas Saku Suurhallis 29.-31. maini toimunud evangeelsest üritusest - Lootuse festivalist, kus esinesid teiste seas lauljad Rapla Maarja- Magdaleena koguduse segakoorist, Hageri koguduse kammerkoorist Lambertus, Kohila babtistikoguduse segakoorist ja Märjamaa Maarja koguduse segakoorist Mariema

  7. Papillary mesothelioma of the albuginea testis

    NARCIS (Netherlands)

    Tjandra, B. S.; Daemen, M. J.; Weil, E. H.

    1994-01-01

    An eleven-year-old boy is presented with symptom of a torsion of the testis. Scrotal exploration revealed a papillary mesothelioma of the tunica albuginea which is extremely rare in childhood. We report 1 case and review the literature

  8. Clerget 100 hp heavy-oil engine

    Science.gov (United States)

    Leglise, Pierre

    1931-01-01

    A complete technical description of the Clerget heavy-oil engine is presented along with the general characteristics. The general characteristics are: 9 cylinders, bore 120 mm, stroke 130 mm, four-stroke cycle engine, rated power limited to 100 hp at 1800 rpm; weight 228 kg; propeller with direct drive and air cooling. Moving parts, engine block, and lubrication are all presented.

  9. UNDESCENDED TESTIS, DIAGNOSIS AND TREATMENT

    Directory of Open Access Journals (Sweden)

    Winarta Lesmana Handrea

    2013-05-01

    Full Text Available Normal 0 false false false EN-US X-NONE X-NONE Undescended testis (UDT or cryptorchidism is one of the commonest abnormalities in male infants. In this anomaly, testes are not located normally in the scrotum. The incidence of UDT is 4-5% of term male infants, and 20-33% of premature male infants. The occurrence of abnormalities of hormones control or anatomy process that is required in the normal process of lowering the testes can cause UDT. UDT can be differentiated into palpable and nonpalpable. The diagnosis of UDT can be known through physical examination. However, if the testes are impalpable, laparoscopy can be done to determine the position of the testis. Hormonal therapy to overcome UDT is still under controversy. The action that often done is surgery, called orchidopexy. The most serious complication of orchidopexy is testicular atrophy. It occurs in a small percentage, which is about 5-10%. Infertility may occur in 1 to 3 of 4 adult males and the risk of occurrence of malignancies is increased by as much as 5-10 times higher in men with a history of UDT. /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin-top:0in; mso-para-margin-right:0in; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0in; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;}

  10. Verification of Goal-Oriented HP-Adaptivity

    National Research Council Canada - National Science Library

    Paszynski, M; Demkowicz, L; Pardo, D

    2005-01-01

    .... The analytical solution has been used to validate a goaloriented hp adaptive strategy based on a simultaneous solution of a dual problem, and an hp strategy based on minimizing the projection-based...

  11. HP advances Grid Strategy for the adaptive enterprise

    CERN Multimedia

    2003-01-01

    "HP today announced plans to further enable its enterprise infrastructure technologies for grid computing. By leveraging open grid standards, HP plans to help customers simplify the use and management of distributed IT resources. The initiative will integrate industry grid standards, including the Globus Toolkit and Open Grid Services Architecture (OGSA), across HP's enterprise product lines" (1 page).

  12. Heterochromatin marks HP1γ, HP1α and H3K9me3, and DNA damage response activation in human testis development and germ cell tumours

    DEFF Research Database (Denmark)

    Bartkova, J; Moudry, P; Hodny, Z

    2011-01-01

    of germ cells vs. somatic tissues in early tumourigenesis. Conceptually interesting findings were that subsets of human cells in vivo proliferate despite enhanced heterochromatinization, and that cells can strongly express even multiple heterochromatin features in the absence of functional retinoblastoma...

  13. Heterochromatin marks HP1gamma, HP1alpha and H3K9me3, and DNA damage response activation in human testis development and germ cell tumours

    Czech Academy of Sciences Publication Activity Database

    Bartkova, J.; Moudrý, Pavel; Hodný, Zdeněk; Lukas, J.; Rajpert-De Meyts, E.; Bartek, Jiří

    2011-01-01

    Roč. 34, 4 Pt 2 (2011), e103-e113 ISSN 0105-6263 R&D Projects: GA ČR GA301/08/0353 Grant - others:Lundbeck Foundation(DK) R13-A1287; EU FP7(XE) TRIREME 223575 Institutional research plan: CEZ:AV0Z50520514 Keywords : heterochromatinization * DNA damage response * germinal tumours Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.591, year: 2011

  14. Activin receptor subunits in normal and dysfunctional adult human testis

    DEFF Research Database (Denmark)

    Dias, V; Meachem, S; Rajpert-De Meyts, E

    2008-01-01

    The cellular sites of activin action and its regulation in the normal and dysfunctional adult human testis are unknown.......The cellular sites of activin action and its regulation in the normal and dysfunctional adult human testis are unknown....

  15. Development and descent of the testis in relation to cryptorchidism

    DEFF Research Database (Denmark)

    Virtanen, Helena E; Cortes, Dina; Rajpert-De Meyts, Ewa

    2007-01-01

    The testis descends in two phases. Animal studies suggest, that the transabdominal descent of the testis depends on the insulin-like hormone 3 (INSL3). Androgens are important in the inguinoscrotal testicular descent in animals and humans. In general, the cause of cryptorchidism is unknown...... and the aetiology is possibly multifactorial. Histological changes in cryptorchid testes demonstrate disturbed development. Conclusion: Since testicular descent is regulated by testis-derived hormones, cryptorchidism may reflect a functional defect of the testis....

  16. Tunica vaginalis hydrocele of reposited testis

    Directory of Open Access Journals (Sweden)

    Tiwari Manish

    2009-01-01

    Full Text Available Tunica vaginalis hydrocele (TVH is a relatively common entity. The procedure of testicular reposition in a subcutaneous pouch following severe scrotal avulsion injury is also a standard line of care. Though, the occurrence of TVH in such a reposited testis is extremely rare clinical presentation. We herein report a case of 55-year male patient who presented with swelling over medial aspect of left upper thigh which was diagnosed as TVH of reposited testis on detailed assessment. The patient was managed on the usual lines of management protocols of TVH with successful outcome.

  17. Absent testis: does it exist in 46,XY males?

    African Journals Online (AJOL)

    Annals of Pediatric Surgery 2013, 9:79–80. Keywords: absent testis, undescended testis, vanishing testis. Al Noor Hospital, Abu Dhabi, UAE. Correspondence to Mohamed Amin ElGohary, FRCS, Al Noor Hospital,. PO Box 48481, Abu Dhabi, UAE. Tel: + 971 506 225 532; fax: + 971 249 44470; e-mail: amingoh@gmail.com.

  18. COOLII code conversion from Cyber to HP

    International Nuclear Information System (INIS)

    Lee, Hae Cho; Kim, Hee Kyung

    1996-06-01

    COOLII computer code determines the time required to cooldown the plant from shutdown cooling system initiation condition to cold shutdown or refueling condition. Required time for cooldown is calculated under the various assumption on shutdown cooling heat exchanger(SDCHX) availability, reactor coolant system (RCS) low pressure safety injection(LPSI) flowrate. RCS cooldown rates and component cooling system flow rates. This report firstly describes detailed work carried out for installation of COOLII on HP 9000/700 series as well as relevant code validation results. Attached is a report on software verification and validation results. 7 refs. (Author) .new

  19. Free radicals in adolescent varicocele testis.

    Science.gov (United States)

    Romeo, Carmelo; Santoro, Giuseppe

    2014-01-01

    We examine the relationship between the structure and function of the testis and the oxidative and nitrosative stress, determined by an excessive production of free radicals and/or decreased availability of antioxidant defenses, which occur in the testis of adolescents affected by varicocele. Moreover, the effects of surgical treatment on oxidative stress were provided. We conducted a PubMed and Medline search between 1980 and 2014 using "adolescent," "varicocele," "free radicals," "oxidative and nitrosative stress," "testis," and "seminiferous tubules" as keywords. Cross-references were checked in each of the studies, and relevant articles were retrieved. We conclude that increased concentration of free radicals, generated by conditions of hypoxia, hyperthermia, and hormonal dysfunction observed in adolescent affected by varicocele, can harm germ cells directly or indirectly by influencing nonspermatogenic cells and basal lamina. With regard to few available data in current literature, further clinical trials on the pre- and postoperative ROS and RNS levels together with morphological studies of the cellular component of the testis are fundamental for complete comprehension of the role played by free radicals in the pathogenesis of adolescent varicocele and could justify its pharmacological treatment with antioxidants.

  20. Wiener-Granger causality in QT-HP variability interactions.

    Science.gov (United States)

    Porta, Alberto; Bari, Vlasta; Marchi, Andrea; De Maria, Beatrice; Cerutti, Sergio

    2015-08-01

    The study exploits a Wiener-Granger causality (WGC) approach in the time domain to assess directionality of the dynamical interactions between QT interval and heart period (HP) during a graded head-up tilt protocol challenging the cardiovascular control as a function of the tilt table inclination. QT interval and HP are approximated from the surface ECG as the temporal distance between the R-wave apex and T-wave offset and between two consecutive R-wave peaks respectively. The adopted WGC approach accounts for the confounding effect of respiration (RESP) affecting both QT and HP. Causality ratios (CRs) from HP to QT given RESP (CRHP→QT|RESP) and from QT to HP given RESP (CRQT→HP|RESP) were computed and their significance was tested via F-test. We found that, regardless of the tilt table angle, CRHP→QT|RESP is significant, while CRQT→HP|RESP is negligible. CRHP→QT|RESP showed a trend towards a decrease with tilt table angle. These findings suggested that the causal direction from HP over QT is dominant compared to the reverse one and sympathetic activation induced a QT-HP uncoupling.

  1. COAST code conversion from Cyber to HP

    International Nuclear Information System (INIS)

    Lee, Hae Cho

    1996-04-01

    The transient thermal hydraulic behavior of reactor coolant system in a nuclear power plant following loss of coolant flow is analyzed by use of COAST digital computer code. COAST calculates individual loop flow rates and steam generator pressure drops is a function of time following coast-down of any number of reactor coolant pumps. This report firstly describes detailed work carried out for installation of COAST on HP 9000/700 series and code validation results after installation. Secondly, a series of work is also describes in relation to installation of COAST on Apollo DN10000 series as well as relevant code validation results. Attached is a report on software verification and validation results. 7 refs. (Author) .new

  2. Holomorphic extension of generalizations of Hp functions

    Directory of Open Access Journals (Sweden)

    Richard D. Carmichael

    1985-01-01

    Full Text Available In recent analysis we have defined and studied holomorphic functions in tubes in ℂn which generalize the Hardy Hp functions in tubes. In this paper we consider functions f(z, z=x+iy, which are holomorphic in the tube TC=ℝn+iC, where C is the finite union of open convex cones Cj, j=1,…,m, and which satisfy the norm growth of our new functions. We prove a holomorphic extension theorem in which f(z, z ϵ TC, is shown to be extendable to a function which is holomorphic in T0(C=ℝn+i0(C, where 0(C is the convex hull of C, if the distributional boundary values in 𝒮′ of f(z from each connected component TCj of TC are equal.

  3. Phantom testis syndrome: prevalence, phenomenology and putative mechanisms.

    Science.gov (United States)

    Pühse, Gerald; Wachsmuth, Julia Urte; Kemper, Sebastian; Husstedt, Ingo W; Kliesch, Sabine; Evers, Stefan

    2010-02-01

    Chronic phantom pain has been found in up to 78% of limb amputees and is a major complication of limb amputation. Less is known about phantom phenomena after the amputation of other, i.e. visceral, parts of the body. In a retrospective design, we identified 539 patients in whom one testis was removed between 1995 and 2005. The operative technique was a unilateral standard radical inguinal orchiectomy. The underlying pathology in all cases was a testicular germ cell tumour. All patients received a detailed questionnaire asking about the occurrence of phantom testis pain (pain felt in the removed testis), phantom testis sensations (non-painful sensations as if the removed testis was still intact) and hallucinations (illusionary perceptions on the removed testis). Furthermore, we asked about the occurrence and clinical presentation of pain before and after surgery and about pre-operative testicular pain. Out of 238 respondents, 125 patients (53%) reported any kind of phantom experience. The prevalence of phantom testis pain was 25% (60/238), non-painful phantom sensations 16% (37/238) and male gonad hallucinations 12% (28/238). Patients with phantom symptoms reported pre-operative pain in the removed testis more often than patients without phantom symptoms. This study presents first data on the clinical characteristics and possible mechanisms of the phantom testis syndrome after surgical removal of one testis.

  4. An update of the macaque testis proteome

    Directory of Open Access Journals (Sweden)

    Tao Zhou

    2015-12-01

    Full Text Available The genome sequence of rhesus macaque is a draft version with many errors and is lack of Y chromosome annotation. In the present dataset, we reanalyzed the previously published macaque testis proteome. We searched for refined protein sequences, potential Y chromosome proteins and transcripts predicted proteins in addition to the latest Ensembl protein sequences of macaque. A total of 74,433 peptides corresponding to 9247 protein groups were identified, and the data are supplied in this paper. The updated version of macaque testis proteome provided evidences for predicted genes or transcripts at the peptide level. It can be used for further in-depth proteogenomic annotation of macaque genome and is useful for studying the mechanisms of macaque spermatogenesis.

  5. Thyroid hormone and leptin in the testis

    Directory of Open Access Journals (Sweden)

    Cristiane Fonte Ramos

    2014-11-01

    Full Text Available Leptin is primarily expressed in white adipose tissue; however, it is expressed in the hypothalamus and reproductive tissues as well. Leptin acts by activating the leptin receptors (Ob-Rs. Additionally, the regulation of several neuroendocrine and reproductive functions, including the inhibition of glucocorticoids and enhancement of thyroxine and sex hormone concentrations in humans and mice are leptin functions. It has been suggested that thyroid hormones (TH could directly regulate leptin expression. Additionally, hypothyroidism compromises the intracellular integration of leptin signaling specifically in the arcuate nucleus. Two TH receptor isoforms are expressed in the testis, TRa and TRb, with TRa being the predominant one that is present in all stages of development. The effects of TH involve the proliferation and differentiation of Sertoli and Leydig cells during development, spermatogenesis and steroidogenesis. In this context, TH disorders are associated with sexual dysfunction. An endocrine and/or direct paracrine effect of leptin on the gonads inhibits testosterone production in Leydig cells. Further studies are necessary to clarify the effects of both hormones in the testis during hypothyroidism. The goal of this review is to highlight the current knowledge regarding leptin and TH in the testis.

  6. Goal-Oriented hp-Adaptivity for Elliptic Problems

    Czech Academy of Sciences Publication Activity Database

    Šolín, Pavel; Demkowicz, L.

    2004-01-01

    Roč. 193, 6-8 (2004), s. 449-468 ISSN 0045-7825 R&D Projects: GA ČR GP102/01/D114 Keywords : hp-finite elements * hp-adaptivity * Dual problem Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering Impact factor: 1.263, year: 2004

  7. Kihutav politseijuht Antropov testis Saaremaal Hummerit / Risto Berendson, Tuuli Koch

    Index Scriptorium Estoniae

    Berendson, Risto, 1975-

    2003-01-01

    Politsei pressishefi sõnul testis politseijuht Robert Antropov Saaremaal julgestuspolitsei tarbeks maastikuautot Hummer. Endise politseijuhi Ain Seppiku kommentaare. Tabel: Tavaline politseimaastur võrreldes Hummeriga

  8. Transcriptional profiling of type II toxin-antitoxin genes of Helicobacter pylori under different environmental conditions: identification of HP0967-HP0968 system

    Directory of Open Access Journals (Sweden)

    MIGUEL A DE LA CRUZ

    2016-11-01

    Full Text Available Helicobacter pylori is a Gram-negative bacterium that colonizes the human gastric mucosa and is responsible for causing peptic ulcers and gastric carcinoma. The expression of virulence factors allows the persistence of H. pylori in the stomach, which results in a chronic, sometimes uncontrolled inflammatory response. Type II toxin-antitoxin systems have emerged as important virulence factors in many pathogenic bacteria. Three type II toxin-antitoxin (TA systems have previously been identified in the genome of H. pylori 26695: HP0315-HP0316, HP0892-HP0893, and HP0894-HP0895. Here we characterized a heretofore undescribed type II TA system in H. pylori, HP0967-HP0968, which is encoded by the bicistronic operon hp0968-hp0967 and belongs to the Vap family. The predicted HP0967 protein is a toxin with ribonuclease activity whereas HP0968 is an antitoxin that binds to its own regulatory region. We found that all type II TA systems were expressed in H. pylori during early stationary growth phase, and differentially expressed in the presence of urea, nickel, and iron, although the hp0968-hp0967 pair was the most affected under these environmental conditions. Transcription of hp0968-hp0967 was strongly induced in a mature H. pylori biofilm and when the bacteria interacted with AGS epithelial cells. Kanamycin and chloramphenicol considerably boosted transcription levels of all the four type II TA systems. The hp0968-hp0967 TA system was the most frequent among 317 H. pylori strains isolated from all over the world. This study is the first report on the transcription of type II TA genes in H. pylori under different environmental conditions. Our data show that the HP0967 and HP0968 proteins constitute a bona fide type II TA system in H. pylori, whose expression is regulated by environmental cues, which are relevant in the context of infection of the human gastric mucosa.

  9. Evaluation of HP0605 and HP0971 genes of efflux pumps in Helicobacter pylori resistance to Metronidazole

    Directory of Open Access Journals (Sweden)

    Mohammad hasan Shirazi

    2009-12-01

    Full Text Available Background: The presence of antibiotic resistance has been reported in H.pylori and it is a major cause of treatment failure. Five families of multidrug efflux pumps are defined in bacteria and resistance-nodulation-division (RND pumps are found mainly in gram negative bacteria. TolC is one of RND pump components and play a critical role in drug resistance. It hasn’t been established that RND family has a role in drug resistance in H.pylori. In this study, we assessed the role of two efflux genes in resistant to metronidazole in H.pylori by evaluation of overexpression TolC genes by RT-PCR method. Methods: In five metronidazole resistant strains of H.pylori, total RNA was extracted. RNA treated with DNase and RNA reverse transcribed to cDNA. Aliquots of the cDNA solution were assayed by RT- PCR for HP0605 and HP0971 genes. The levels of mRNA expression were evaluated by densitometry analysis. Results: All five strains displayed overexpression for HP0605 basis of increased concentration of metronidazole. Three strains showed transcripts for HP0971. One of these had transcripts for HP0971 only in Metronidazole concentration equaled to 16 µg/ml but two strains overexpressed adapt to increase concentration of metronidazole. Conclusion: According to current study, HP0605 and HP0971 genes overexpressed due to increase metronidazole. So, increasing of Metronidazole affects in H.pylori΄s efflux system in transcription level.

  10. Ectopic Splenic Tissue in the Testis: A Case Report

    African Journals Online (AJOL)

    Ectopic SplENic tiSSUE iN thE tEStiS associated with bilateral cryptorchidism and hypospadias4. Brasch et al. reported a case involving the right testis5. Ultrasonography, scintigraphy (with technetium-99m) and single positron emission computerized tomography are considered the best diagnostic tools for this condition2,3.

  11. Testicular development and relationship between body weight, testis ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-03-20

    Mar 20, 2009 ... testicular components, absolute testis weight and body weight in boars. There is evidence in literature that the volume percentage occupied by the spermatogenetic tissues increased with age, body weight and testis weight in boars (Allrich et al., 1983; Harder et al., 1995). These reports seem to indicate that ...

  12. Reliability of hypertrophy of the contralateral testis in prediction of ...

    African Journals Online (AJOL)

    Background: The hypertrophied testis can predict the status of the impalpable contralateral one. The aim of this study was to assess the accuracy of contralateral testicular hypertrophy for predicting the presence or absence of impalpable undescended testis (UDT) in Egyptian boys. Patients and methods: This study was ...

  13. Molecular cloning and characterization of a novel human testis ...

    Indian Academy of Sciences (India)

    spermatogenesis-related gene 8 (SRG8). Materials and methods. Data-mining of UniGene database. In this study, the DDD tool was used according to the database instructions. Nine testis-derived cDNA libraries. Keywords. digital differential display; testis; real-time PCR; Northern blot. Journal of Genetics, Vol. 85, No.

  14. Prevalence of Undescended Testis and its Associated Factors ...

    African Journals Online (AJOL)

    Prevalence of Undescended Testis and its Associated Factors among under-fives seen at Reproductive and Child Health Clinic in Ifakara, Tanzania. ... The male sexual differentiation and development is important for the normal reproductive life span. Similarly, risk of carcinoma of testis will be prevented, if early diagnosis of ...

  15. Management of supernumerary testis: a rare case of polyorchidism ...

    African Journals Online (AJOL)

    Supernumeracy testis is frequently associated with undescended testis, testicular torsion, inguinal hernia, and hydrocele. Management is controversial with some advocating for orchidectomy due to concerns of malignancy. The family was educated about the risk for malignancy and the importance of regular follow-up and ...

  16. HP fotovõistluse finalistid veebis / Henrik Roonemaa

    Index Scriptorium Estoniae

    Roonemaa, Henrik

    2002-01-01

    Hewlett-Packardi koduleheküljel on üleval rahvusvahelise digitaalfotograafia konkursi HP Invision Digital Photography Awards 2002 finalistide tööd, nende hulgas Katrin Arraku foto "Minu kala Vallo"

  17. Imaging of mesothelioma of tunica vaginalis testis

    Energy Technology Data Exchange (ETDEWEB)

    Bertolotto, M. [University of Trieste, Department of Radiology, Trieste (Italy); Boulay-Coletta, I. [Fondation Hopital Saint Joseph, Service d' Imagerie Medical, Paris (France); Butini, R. [Ospedale S. Giacomo, Department of Radiology, Castelfranco Veneto, TV (Italy); Dudea, S.M. [Univ. Med. Pharm. ' ' Iuliu Hatieganu' ' , Department of Radiology, Cluj-Napoca (Romania); Grenier, N. [Pellegrin Hospital, Department of Radiology, Bordeaux (France); Oltmanns, G. [University Hospital of North Norway, Department of Radiology, Tromsoe (Norway); Ramchandani, P. [University of Pennsylvania, Department of Radiology, Perelman School of Medicine, Philadelphia, PA (United States); Stein, M.W. [Montefiore Medical Center, Department of Radiology, Albert Einstein College of Medicine, Bronx, NY (United States); Valentino, M. [Sant' Antonio Hospital, Department of Radiology, Tolmezzo, UD (Italy); Derchi, Lorenzo E. [University of Genoa, Department of Health Sciences, Genova (Italy); IRCCS Azienda Ospedaliera Universitaria San Martino IST, Radiologia d' Urgenza, Genova (Italy)

    2016-03-15

    To describe the imaging findings in a series of patients with mesothelioma of the tunica vaginalis testis. We reviewed clinical data, imaging findings and follow-up information in a series of 10 pathology-proven cases of mesothelioma (all had US; 2 had MR) of the tunica vaginalis. A variety of patterns could be observed, the most common (5/10) being a hydrocele with parietal, solid and hypervascular vegetations; one patient had a septated hydrocele with hypervascular walls; one had multiple, solid nodules surrounded by a small, physiological quantity of fluid; one a cystic lesion with thick walls and vegetations compressing the testis; two had a solid paratesticular mass. MR showed multiple small nodules on the surface of the tunica vaginalis in one case and diffuse thickening and vegetations in the other one; lesions had low signal intensity on T2-w images and were hypervascular after contrast injection. A preoperative diagnosis of mesotheliomas presenting as solid paratesticular masses seems very difficult with imaging. On the contrary, the diagnosis must be considered in patients in whom a hydrocele with parietal vegetations is detected, especially if these show high vascularity. (orig.)

  18. Tonopah Test Range EGS graphics tracking display system: HP370

    Energy Technology Data Exchange (ETDEWEB)

    Meyer, R.H.; Bauhs, K.C.

    1994-08-01

    This report describes the HP370 component of the Enhanced Graphics System (EGS) used at Tonopah Test Range (TTR). Selected Radar data is fed into the computer systems and the resulting tracking symbols are displayed on high-resolution video monitors in real time. These tracking symbols overlay background maps and are used for monitoring/controlling various flight vehicles. This report discusses both the operational aspects and the internal configuration of the HP370 Workstation portion of the EGS system.

  19. Molecular and cellular endocrinology of the testis

    International Nuclear Information System (INIS)

    Stefanini, M.; Conti, M.; Geremia, R.; Ziparo, E.

    1986-01-01

    This volume contains the Proceedings of the IV European Workshop on Molecular and Cellular Endocrinology of the Testis held in Capri (Italy) between the 9th and 12th April 1986. The workshop was organized in several symposia related to some of the most relevant aspects of the regulation of testicular function. Main topics were the role of cell interactions, the mechanisms of signal transduction, gene expression and metabolic response of somatic cells as well as differentiation of germ cells. One session was devoted to prostaglandins in the male reproductive system and to brief discussions on interstitial fluid and on antispermatogenic compounds. In this book only the main lectures and some selected short papers are presented. (Auth.)

  20. Aggressive surgical management of adenocarcinoma of the rete testis

    Directory of Open Access Journals (Sweden)

    Avinash Maganty

    2018-01-01

    Full Text Available Adenocarcinoma of the rete testis is a rare and aggressive malignancy arising from the epididymal epithelium. We present a case of a 66-year-old male who presented with left testis mass. Histopathological analysis of orchiectomy specimen was consistent with adenocarcinoma of the rete testis. Subsequent retroperitoneal lymph node dissection revealed metastatic disease not detected on preoperative PET-CT. Prior reports have suggested poor response rates to both systemic chemotherapy and radiation therapy. Aggressive surgical management of the retroperitoneum should be considered even in clinically node-negative patients given the paucity of other effective treatment regimens.

  1. Electron beam inspection of 16nm HP node EUV masks

    Science.gov (United States)

    Shimomura, Takeya; Narukawa, Shogo; Abe, Tsukasa; Takikawa, Tadahiko; Hayashi, Naoya; Wang, Fei; Ma, Long; Lin, Chia-Wen; Zhao, Yan; Kuan, Chiyan; Jau, Jack

    2012-11-01

    EUV lithography (EUVL) is the most promising solution for 16nm HP node semiconductor device manufacturing and beyond. The fabrication of defect free EUV mask is one of the most challenging roadblocks to insert EUVL into high volume manufacturing (HVM). To fabricate and assure the defect free EUV masks, electron beam inspection (EBI) tool will be likely the necessary tool since optical mask inspection systems using 193nm and 199nm light are reaching a practical resolution limit around 16nm HP node EUV mask. For production use of EBI, several challenges and potential issues are expected. Firstly, required defect detection sensitivity is quite high. According to ITRS roadmap updated in 2011, the smallest defect size needed to detect is about 18nm for 15nm NAND Flash HP node EUV mask. Secondly, small pixel size is likely required to obtain the high sensitivity. Thus, it might damage Ru capped Mo/Si multilayer due to accumulated high density electron beam bombardments. It also has potential of elevation of nuisance defects and reduction of throughput. These challenges must be solved before inserting EBI system into EUV mask HVM line. In this paper, we share our initial inspection results for 16nm HP node EUV mask (64nm HP absorber pattern on the EUV mask) using an EBI system eXplore® 5400 developed by Hermes Microvision, Inc. (HMI). In particularly, defect detection sensitivity, inspectability and damage to EUV mask were assessed. As conclusions, we found that the EBI system has capability to capture 16nm defects on 64nm absorber pattern EUV mask, satisfying the sensitivity requirement of 15nm NAND Flash HP node EUV mask. Furthermore, we confirmed there is no significant damage to susceptible Ru capped Mo/Si multilayer. We also identified that low throughput and high nuisance defect rate are critical challenges needed to address for the 16nm HP node EUV mask inspection. The high nuisance defect rate could be generated by poor LWR and stitching errors during EB writing

  2. hp-finite element methods for singular perturbations

    CERN Document Server

    Melenk, Jens M

    2002-01-01

    Many partial differential equations arising in practice are parameter-dependent problems that are of singularly perturbed type. Prominent examples include plate and shell models for small thickness in solid mechanics, convection-diffusion problems in fluid mechanics, and equations arising in semi-conductor device modelling. Common features of these problems are layers and, in the case of non-smooth geometries, corner singularities. Mesh design principles for the efficient approximation of both features by the hp-version of the finite element method (hp-FEM) are proposed in this volume. For a class of singularly perturbed problems on polygonal domains, robust exponential convergence of the hp-FEM based on these mesh design principles is established rigorously.

  3. FlyTED: the Drosophila Testis Gene Expression Database

    OpenAIRE

    Zhao, Jun; Klyne, Graham; Benson, Elizabeth; Gudmannsdottir, Elin; White-Cooper, Helen; Shotton, David

    2009-01-01

    FlyTED, the Drosophila Testis Gene Expression Database, is a biological research database for gene expression images from the testis of the fruit fly Drosophila melanogaster. It currently contains 2762 mRNA in situ hybridization images and ancillary metadata revealing the patterns of gene expression of 817 Drosophila genes in testes of wild type flies and of seven meiotic arrest mutant strains in which spermatogenesis is defective. This database has been built by adapting a widely used digita...

  4. A methodology to split the total cumulative Hp(10) dose into the Hp(10)idoses received during various procedures performed by interventional cardiologist.

    Science.gov (United States)

    Domienik, Joanna

    2017-03-01

    The methodology describing how to split the cumulative Hp(10) dose of interventional cardiologists into Hp(10) i doses received during procedures of various types based on procedure-specific ELDO coefficients and Hp(3) doses per procedure is presented. The appropriate equations for Hp(10) i (Hp(10) for procedure type i), depending on the number of various procedure types (i  =  1 … 4) performed by a particular physician, are derived. The methodology can be applied to whole-body doses measured on the lead apron and therefore can be used for optimisation of work practices in those catheterisation labs where routine dosimeter is worn above the apron.

  5. Laparoscopic diagnosis and treatment of nonpalpable testis

    Directory of Open Access Journals (Sweden)

    Francisco T. Denes

    2008-06-01

    Full Text Available INTRODUCTION: Treatment of the cryptorchid testicle is justified due to the increased risk of infertility and malignancy as well as the risk of testicular trauma and psychological stigma on patients and their parents. Approximately 20% of cryptorchid testicles are nonpalpable. In these cases, the videolaparoscopic technique is a useful alternative method for diagnosis and treatment. MATERIALS AND METHODS: We present data concerning 90 patients submitted to diagnostic laparoscopy for impalpable testicles. Forty-six patients (51.1% had intra-abdominal gonads. In 25 testicles of 19 patients, we performed a two stage laparoscopic Fowler-Stephens orchiopexy. The other 27 patients underwent primary laparoscopic orchiopexy, in a total of 29 testicles. RESULTS: We obtained an overall 88% success rate with the 2 stage Fowler-Stephens approach and only 33% rate success using one stage Fowler-Stephens surgery with primary vascular ligature. There was no intraoperative complication in our group of patients. In the laparoscopic procedures, the cosmetic aspect is remarkably more favorable as compared to open surgeries. Hospital stay and convalescence were brief. CONCLUSIONS: In pediatric age group, the laparoscopic approach is safe and feasible. Furthermore, the laparoscopic orchiopexy presents excellent results in terms of diagnosis and therapy of the impalpable testis, which is why this technique has been routinely incorporated in our Department.

  6. Spectral/ hp element methods: Recent developments, applications, and perspectives

    Science.gov (United States)

    Xu, Hui; Cantwell, Chris D.; Monteserin, Carlos; Eskilsson, Claes; Engsig-Karup, Allan P.; Sherwin, Spencer J.

    2018-02-01

    The spectral/ hp element method combines the geometric flexibility of the classical h-type finite element technique with the desirable numerical properties of spectral methods, employing high-degree piecewise polynomial basis functions on coarse finite element-type meshes. The spatial approximation is based upon orthogonal polynomials, such as Legendre or Chebychev polynomials, modified to accommodate a C 0 - continuous expansion. Computationally and theoretically, by increasing the polynomial order p, high-precision solutions and fast convergence can be obtained and, in particular, under certain regularity assumptions an exponential reduction in approximation error between numerical and exact solutions can be achieved. This method has now been applied in many simulation studies of both fundamental and practical engineering flows. This paper briefly describes the formulation of the spectral/ hp element method and provides an overview of its application to computational fluid dynamics. In particular, it focuses on the use of the spectral/ hp element method in transitional flows and ocean engineering. Finally, some of the major challenges to be overcome in order to use the spectral/ hp element method in more complex science and engineering applications are discussed.

  7. On a Weak Discrete Maximum Principle for hp-FEM

    Czech Academy of Sciences Publication Activity Database

    Šolín, Pavel; Vejchodský, Tomáš

    -, č. 209 (2007), s. 54-65 ISSN 0377-0427 R&D Projects: GA ČR(CZ) GA102/05/0629 Institutional research plan: CEZ:AV0Z20570509; CEZ:AV0Z10190503 Keywords : discrete maximum principle * hp-FEM Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering Impact factor: 0.943, year: 2007

  8. hp Spectral element methods for three dimensional elliptic problems ...

    Indian Academy of Sciences (India)

    125, No. 3, August 2015, pp. 413–447. c Indian Academy of Sciences h-p Spectral element methods for three dimensional elliptic problems on non-smooth domains, Part-II: Proof of stability theorem. P DUTT1, AKHLAQ HUSAIN2,∗, A S VASUDEVA MURTHY3 and C S UPADHYAY4. 1Department of Mathematics & Statistics ...

  9. Discrete frequency identification using the HP 5451B Fourier analyser

    International Nuclear Information System (INIS)

    Holland, L.; Barry, P.

    1977-01-01

    The frequency analysis by the HP5451B discrete frequency Fourier analyser is studied. The advantages of cross correlation analysis to identify discrete frequencies in a background noise are discussed in conjuction with the elimination of aliasing and wraparound error. Discrete frequency identification is illustrated by a series of graphs giving the results of analysing 'electrical' and 'acoustical' white noise and sinusoidal signals [pt

  10. Printerite riigihanked libisevad HP-le / Askur Alas

    Index Scriptorium Estoniae

    Alas, Askur, 1973-

    2003-01-01

    AS Rapo turundusjuhi Andres Kaevu hinnangul oli maksuameti korraldatud riigihanke eesmärk osta HP printereid ning välistada konkurendid. Tema sõnul võimaldaksid mõne teise firma printerid riigil raha kokku hoida. Kommenteerib Konica Minolta Baltia Eesti filiaali juhataja Margus Vaino

  11. EV and HP Providing Ancillary Services in the Nordic Region

    DEFF Research Database (Denmark)

    Liu, Zhaoxi; Wu, Qiuwei

    This report covers the analysis of the electric vehicle (EV) and heat pump (HP) providing ancillary services to the power system of the Nordic region including Denmark, Finland, Norway and Sweden. The analysis is to investigate the feasibility of EVs and HPs to serve as demand as frequency reserve...

  12. Undescended testis: how extensive should the work up be?

    Science.gov (United States)

    Shera, Altaf Hussain; Baba, Aejaz Ahsan; Gupta, Shyam Kumar; Gupta, Geetanjali; Sherwani, Afak Yusuf

    2010-01-01

    The aim of this study was to highlight various anomalies associated with undescended testis and to determine how much work up is necessary for this condition. The study was conducted in the department of Pediatric Surgery SKIMS Srinagar, Kashmir. All patients between 0-14 years of age who attended out patient department (OPD) from January 2002 to December 2003 with maldescent of testes were included in the study. Detailed relevant history and physical examination findings were recorded in all the cases. Baseline investigations were performed along with ultrasonography of the abdomen. In relevant cases other investigations like intravenous urography, micturating cystourethrography, CT scan and laparoscopy were performed as and when indicated. A total of 250 cases of undescended testis were registered during this period. Maximum number of cases were in the age group of 5-10 years. In 130 (52%) cases the right testis was undescended while 75 (30%) had left sided undescended testis and 45 (18%) had bilateral undescended testis. Maldescended testis comprised 11% of the admissions. The majority of cases were having gestational age of 37 weeks or more. The associated anomalies picked up on investigations included duplication of upper urinary tract (3.2%), hydronephrosis and polycystic kidney (0.8% each), horseshoe kidney, ectopic kidney, crossed renal ectopia (0.4% each) Posterior urethral valves, Prune belly syndrome (0.4%) and spina bifida (0.4%). On detailed clinical examination of genitalia several abnormalities were picked which included hydrocele, hypospadias, hernia, chordee, micropenis and ambiguous genitalia. We recommend ultrasonography to be done in all cases of undescended testis in addition to a thorough history and physical examination. Intravenous pyelography, micturating cystourethrogram, CT scan and other investigations should be performed selectively based on history, physical examination or ultrasound findings.

  13. Undesended testis: How extensive should the work up be?

    Directory of Open Access Journals (Sweden)

    Shera Altaf

    2010-01-01

    Full Text Available Aim: The aim of this study was to highlight various anomalies associated with undescended testis and to determine how much work up is necessary for this condition. Material and Methods: The study was conducted in the department of Pediatric Surgery SKIMS Srinagar, Kashmir. All patients between 0-14 years of age who attended out patient department (OPD from January 2002 to December 2003 with maldescent of testes were included in the study. Detailed relevant history and physical examination findings were recorded in all the cases. Baseline investigations were performed along with ultrasonography of the abdomen. In relevant cases other investigations like intravenous urography, micturating cystourethrography, CT scan and laparoscopy were performed as and when indicated. Results: A total of 250 cases of undescended testis were registered during this period. Maximum number of cases were in the age group of 5-10 years. In 130 (52% cases the right testis was undescended while 75 (30% had left sided undescended testis and 45 (18% had bilateral undescended testis. Maldescended testis comprised 11% of the admissions. The majority of cases were having gestational age of 37 weeks or more. The associated anomalies picked up on investigations included duplication of upper urinary tract (3.2%, hydronephrosis and polycystic kidney (0.8% each, horseshoe kidney, ectopic kidney, crossed renal ectopia (0.4% each Posterior urethral valves, Prune belly syndrome (0.4% and spina bifida (0.4%. On detailed clinical examination of genitalia several abnormalities were picked which included hydrocele, hypospadias, hernia, chordee, micropenis and ambiguous genitalia. Conclusion: We recommend ultrasonography to be done in all cases of undescended testis in addition to a thorough history and physical examination. Intravenous pyelography, micturating cystourethrogram, CT scan and other investigations should be performed selectively based on history, physical examination or

  14. Contralateral testicular biopsy in testis cancer: current concepts and controversies.

    Science.gov (United States)

    Heidenreich, Axel

    2009-11-01

    Of all patients with unilateral testis cancer, approximately 5% harbour testicular intraepithelial neoplasia (TIN) in their contralateral testicle that will progress into an invasive germ cell tumour over time. The accurate diagnosis of TIN by a random two-site surgical testis biopsy and effective therapy by local radiation has led to the concept of a contralateral screening biopsy in all patients with testis cancer. However, screening and preventive treatment are only indicated if the therapeutic outcome of the screened population is improved, and the physiological function of the affected organ is not impaired. Based on a critical review of previous reports, some drawbacks of this policy have to be considered and question the routine indication for contralateral testis biopsy: (i) all TIN-negative patients still have to undergo meticulous follow-up for metachronous testis cancer due to a false negative biopsy rate of 0.5-1.0%; (ii) local radiation of TIN results in irreversible infertility due to eradication of spermatogenesis; (iii) local radiation of TIN results in an impairment of endocrine Leydig cell function in 25% of the patients; (iv) therapeutic outcome and prognosis will not be improved in irradiated patients as compared to patients on surveillance; (v) local tumour resection for the management of metachronous testicular cancer represents an effective and viable option. Current reports do not support the strategy of contralateral testis biopsy in all patients with unilateral testicular germ cell tumours. According to the recommendations of the European Germ Cell Cancer Consensus Group, a testis biopsy might be offered to high-risk patients for contralateral TIN (testicular volume <12 mL, history of cryptorchidism, age <30 years).

  15. Thyroid Hormone Role and Economy in the Developing Testis.

    Science.gov (United States)

    Hernandez, Arturo

    2018-01-01

    Thyroid hormones (TH) exhibit pleiotropic regulatory effects on growth, development, and metabolism, and it is becoming increasingly apparent that the developing testis is an important target for them. Testicular development is highly dependent on TH status. Both hypo- and hyperthyroidism affect testis size and the proliferation and differentiation of Sertoli, Leydig, and germ cells, with consequences for steroidogenesis, spermatogenesis, and male fertility. These observations suggest that an appropriate content of TH and by implication TH action in the testis, whether the result of systemic hormonal levels or regulatory mechanisms at the local level, is critical for normal testicular and reproductive function. The available evidence indicates the presence in the developing testis of a number of transporters, deiodinases and receptors that could play a role in the timely delivery of TH action on testicular cells. These include the thyroid hormone receptor alpha (THRA), the MCT8 transporter, the TH-activating deiodinase DIO2, and the TH-inactivating deiodinase DIO3, all of which appear to modulate testicular TH economy and testis outcomes. © 2018 Elsevier Inc. All rights reserved.

  16. HP-1γ Controls High-Affinity Antibody Response to T-Dependent Antigens.

    Science.gov (United States)

    Ha, Ngoc; Pham, Duc-Hung; Shahsafaei, Aliakbar; Naruse, Chie; Asano, Masahide; Thai, To-Ha

    2014-01-01

    In vitro observations suggest a role for the mouse heterochromatin protein 1γ (HP-1γ) in the immune system. However, it has not been shown if and how HP-1γ contributes to immunity in vivo. Here we show that in mice, HP-1γ positively regulates the germinal center reaction and high-affinity antibody response to thymus (T)-dependent antigens by limiting the size of CD8(+) regulatory T-cell (Treg) compartment without affecting progenitor B- or T-cell-development. Moreover, HP-1γ does not control cell proliferation or class switch recombination. Haploinsufficiency of cbx-3 (gene encoding HP-1γ) is sufficient to expand the CD8(+) Treg population and impair the immune response in mice despite the presence of wild-type HP-1α and HP-1β. This is the first in vivo evidence demonstrating the non-redundant role of HP-1γ in immunity.

  17. Testicular microlithiasis in boys and young men with congenital or acquired undescended (ascending) testis

    NARCIS (Netherlands)

    Goede, J.; Hack, W.W.M.; Voort-Doedens, L.M. van der; Pierik, F.H.; Looijenga, L.H.J.; Sijstermans, K.

    2010-01-01

    Purpose: We assessed the prevalence of testicular microlithiasis by ultrasound in boys and young men with congenital or acquired undescended (ascending) testis. Materials and Methods: During followup for testicular growth patients with congenital or acquired undescended (ascending) testis were also

  18. Macrophages Contribute to the Spermatogonial Niche in the Adult Testis

    Directory of Open Access Journals (Sweden)

    Tony DeFalco

    2015-08-01

    Full Text Available The testis produces sperm throughout the male reproductive lifespan by balancing self-renewal and differentiation of spermatogonial stem cells (SSCs. Part of the SSC niche is thought to lie outside the seminiferous tubules of the testis; however, specific interstitial components of the niche that regulate spermatogonial divisions and differentiation remain undefined. We identified distinct populations of testicular macrophages, one of which lies on the surface of seminiferous tubules, in close apposition to areas of tubules enriched for undifferentiated spermatogonia. These macrophages express spermatogonial proliferation- and differentiation-inducing factors, such as colony-stimulating factor 1 (CSF1 and enzymes involved in retinoic acid (RA biosynthesis. We show that transient depletion of macrophages leads to a disruption in spermatogonial differentiation. These findings reveal an unexpected role for macrophages in the spermatogonial niche in the testis and raise the possibility that macrophages play previously unappreciated roles in stem/progenitor cell regulation in other tissues.

  19. A Chaotic Oscillator Based on HP Memristor Model

    Directory of Open Access Journals (Sweden)

    Guangyi Wang

    2015-01-01

    Full Text Available This paper proposes a simple autonomous memristor-based oscillator for generating periodic signals. Applying an external sinusoidal excitation to the autonomous system, a nonautonomous oscillator is obtained, which contains HP memristor model and four linear circuit elements. This memristor-based oscillator can generate periodic, chaotic, and hyperchaotic signals under the periodic excitation and an appropriate set of circuit parameters. It also shows that the system exhibits alternately a hidden attractor with no equilibrium and a self-excited attractor with a line equilibrium as time goes on. Furthermore, some specialties including burst chaos, irregular periodic bifurcations, and nonintermittence chaos of the circuit are found by theoretical analysis and numerical simulations. Finally, a discrete model for the HP memristor is given and the main statistical properties of this memristor-based oscillator are verified via DSP chip experiments and NIST (National Institute of Standards and Technology tests.

  20. Real-time MPEG-1 software decoding on HP workstations

    Science.gov (United States)

    Bhaskaran, Vasudev; Konstantinides, Konstantinos; Lee, Ruby B.

    1995-04-01

    A MPEG1 codec that is capable of real-time playback of video and audio on HP's RISC-based workstations has been developed. Real-time playback is achieved by examining the complete MPEG1 decoding pipeline and optimizing the algorithms used for the various stages of the video and audio decompression process. For video decompression, efficient implementations are derived by examining the huffman decoder, inverse quantizer and inverse DCT as a single system. For audio decompression, by viewing the subband synthesis function in MPEG1 layer I and layer II decoding as a DCT, a fast 32-point DCT suitable for use in MPEG1 audio decompression has been developed. Besides algorithmic enhancements, in order to achieve real-time performance, minor changes to the CPU architecture and the display pipeline architecture were needed. The integration of algorithmic and architectural enhancements results in real-time playback of MPEG1 video and audio on HP's RISC based workstations.

  1. Initial Development of an Electronic Testis Rigidity Tester

    Directory of Open Access Journals (Sweden)

    Petros Mirilas

    2011-01-01

    Full Text Available We aimed to develop our previously presented mechanical device, the Testis Rigidity Tester (TRT, into an electronic system (Electronic Testis Rigidity Tester, ETRT by applying tactile imaging, which has been used successfully with other solid organs. A measuring device, located at the front end of the ETRT incorporates a tactile sensor comprising an array of microsensors. By application of a predetermined deformation of 2 mm, increased pressure alters linearly the resistance of each microsensor, producing changes of voltage. These signals were amplified, filtered, and digitized, and then processed by an electronic collector system, which presented them as a color-filled contour plot of the area of the testis coming into contact with the sensor. Testis models of different rigidity served for initial evaluation of ETRT; their evacuated central spaces contained different, increasing glue masses. An independent method of rigidity measurement, using an electric weight scale and a micrometer, showed that the more the glue injected, the greater the force needed for a 2-mm deformation. In a preliminary test, a single sensor connected to a multimeter showed similar force measurement for the same deformation in these phantoms. For each of the testis models compressed in the same manner, the ETRT system offered a map of pressures, represented by a color scale within the contour plot of the contact area with the sensor. ETRT found certain differences in rigidity between models that had escaped detection by a blind observer. ETRT is easy to use and provides a color-coded “insight“ of the testis internal structure. After experimental testing, it could be valuable in intraoperative evaluation of testes, so that the surgeon can decide about orchectomy or orcheopexy.

  2. Mammalian HP1 Isoforms Have Specific Roles in Heterochromatin Structure and Organization

    Directory of Open Access Journals (Sweden)

    Laia Bosch-Presegué

    2017-11-01

    Full Text Available HP1 is a structural component of heterochromatin. Mammalian HP1 isoforms HP1α, HP1β, and HP1γ play different roles in genome stability, but their precise role in heterochromatin structure is unclear. Analysis of Hp1α−/−, Hp1β−/−, and Hp1γ−/− MEFs show that HP1 proteins have both redundant and unique functions within pericentric heterochromatin (PCH and also act globally throughout the genome. HP1α confines H4K20me3 and H3K27me3 to regions within PCH, while its absence results in a global hyper-compaction of chromatin associated with a specific pattern of mitotic defects. In contrast, HP1β is functionally associated with Suv4-20h2 and H4K20me3, and its loss induces global chromatin decompaction and an abnormal enrichment of CTCF in PCH and other genomic regions. Our work provides insight into the roles of HP1 proteins in heterochromatin structure and genome stability.

  3. Vibration and Stability of 3000-hp, Titanium Chemical Process Blower

    Directory of Open Access Journals (Sweden)

    Les Gutzwiller

    2003-01-01

    Full Text Available This 74-in-diameter blower had an overhung rotor design of titanium construction, operating at 50 pounds per square inch gauge in a critical chemical plant process. The shaft was supported by oil-film bearings and was directdriven by a 3000-hp electric motor through a metal disk type of coupling. The operating speed was 1780 rpm. The blower shaft and motor shaft motion was monitored by Bently Nevada proximity probes and a Model 3100 monitoring system.

  4. Surgical treatment and follow up on undescended testis

    DEFF Research Database (Denmark)

    Thorup, Jørgen Mogens; Cortes, D.

    2009-01-01

    With skill and ability to control a variety of operative techniques, undescended testes can be placed in the scrotum with 90% success rate in palpable testes and 85% in abdominal testes. Long term results are related also to the variability of the disease. A hypothesis that in cryptorchid testes...... number at birth in some cryptorchid testes and Intratubular Germ Cell Neoplasia seen in early childhood. The hypothesis that the abnormal location of the testis exposes the testis to infertility and malignant transformation is supported by the findings of early treatment lowering the risk of both...

  5. Sex cord-gonadal stromal tumor of the rete testis.

    Science.gov (United States)

    Sajadi, Kamran P; Dalton, Rory R; Brown, James A

    2009-01-01

    A 34-year-old tetraplegic patient with suppurative epididymitis was found on follow-up examination and ultrasonography to have a testicular mass. The radical orchiectomy specimen contained an undifferentiated spindled sex cord-stromal tumor arising in the rete testis. Testicular sex cord-stromal tumors are far less common than germ cell neoplasms and are usually benign. The close relationship between sex cords and ductules of the rete testis during development provides the opportunity for these uncommon tumors to arise anatomically within the rete tesis. This undifferentiated sex cord-stromal tumor, occurring in a previously unreported location, is an example of an unusual lesion mimicking an intratesticular malignant neoplasm.

  6. File list: ALL.Gon.05.AllAg.Testis [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.Gon.05.AllAg.Testis hg19 All antigens Gonad Testis ERX096851,ERX096875,SRX66344...441,SRX663438,SRX663451,SRX663439,SRX663445,SRX663442 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/ALL.Gon.05.AllAg.Testis.bed ...

  7. File list: InP.Gon.05.AllAg.Testis [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.Gon.05.AllAg.Testis hg19 Input control Gonad Testis SRX663452,SRX663439,SRX6634...45,SRX663442 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.Gon.05.AllAg.Testis.bed ...

  8. File list: His.Gon.20.AllAg.Testis [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Gon.20.AllAg.Testis mm9 Histone Gonad Testis SRX118394,SRX803711,SRX803710,SRX3...SRX112973,SRX185799,SRX185788 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.Gon.20.AllAg.Testis.bed ...

  9. File list: ALL.Gon.20.AllAg.Testis [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.Gon.20.AllAg.Testis hg19 All antigens Gonad Testis ERX096851,SRX663448,SRX12124...444,SRX663451,SRX663439,SRX663438,SRX663445,SRX663442 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/ALL.Gon.20.AllAg.Testis.bed ...

  10. File list: InP.Gon.10.AllAg.Testis [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.Gon.10.AllAg.Testis mm9 Input control Gonad Testis SRX349389,SRX1011025,SRX1183...X838550,SRX118389 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.Gon.10.AllAg.Testis.bed ...

  11. File list: ALL.Gon.20.AllAg.Testis [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.Gon.20.AllAg.Testis mm9 All antigens Gonad Testis SRX118394,SRX349391,SRX803711...SRX112541,SRX118388,SRX118389,ERX144545,ERX144532,SRX112536,SRX112537 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.Gon.20.AllAg.Testis.bed ...

  12. File list: His.Adl.10.AllAg.Testis [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Adl.10.AllAg.Testis dm3 Histone Adult Testis SRX017854,SRX017856,SRX390502,SRX3...90501 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/His.Adl.10.AllAg.Testis.bed ...

  13. File list: NoD.Gon.50.AllAg.Testis [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.Gon.50.AllAg.Testis hg19 No description Gonad Testis ERX096851,ERX161924,ERX096...870,ERX096849,ERX096875,ERX096874,ERX096842 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/NoD.Gon.50.AllAg.Testis.bed ...

  14. File list: His.Adl.20.AllAg.Testis [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Adl.20.AllAg.Testis dm3 Histone Adult Testis SRX390502,SRX390501,SRX017856,SRX0...17854 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/His.Adl.20.AllAg.Testis.bed ...

  15. File list: Oth.Gon.50.AllAg.Testis [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Gon.50.AllAg.Testis hg19 TFs and others Gonad Testis SRX663441,SRX663451,SRX663...450,SRX663444,SRX663438 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Gon.50.AllAg.Testis.bed ...

  16. File list: InP.Gon.05.AllAg.Testis [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.Gon.05.AllAg.Testis mm9 Input control Gonad Testis SRX112977,SRX349389,SRX10110...X803713,SRX838550 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.Gon.05.AllAg.Testis.bed ...

  17. File list: ALL.Adl.10.AllAg.Testis [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.Adl.10.AllAg.Testis dm3 All antigens Adult Testis SRX017857,SRX017854,SRX017856...,SRX390502,SRX390501,SRX390503 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/ALL.Adl.10.AllAg.Testis.bed ...

  18. File list: NoD.Gon.05.AllAg.Testis [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.Gon.05.AllAg.Testis mm9 No description Gonad Testis ERX144544,ERX144521,ERX2210...144545,ERX144532 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.Gon.05.AllAg.Testis.bed ...

  19. File list: InP.Gon.50.AllAg.Testis [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.Gon.50.AllAg.Testis mm9 Input control Gonad Testis SRX112534,SRX349389,SRX02744...X112541,SRX118389 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.Gon.50.AllAg.Testis.bed ...

  20. File list: Pol.Gon.10.AllAg.Testis [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Gon.10.AllAg.Testis mm9 RNA polymerase Gonad Testis SRX349391,SRX112975,SRX1437...96,SRX237511,SRX237510,SRX244355 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Gon.10.AllAg.Testis.bed ...

  1. File list: ALL.Gon.10.AllAg.Testis [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.Gon.10.AllAg.Testis mm9 All antigens Gonad Testis SRX118383,SRX112538,SRX118394...ERX144545,ERX144553,SRX118378,SRX118389,SRX112536,ERX144532,SRX112537 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.Gon.10.AllAg.Testis.bed ...

  2. File list: His.Adl.50.AllAg.Testis [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Adl.50.AllAg.Testis dm3 Histone Adult Testis SRX390502,SRX390501,SRX017856,SRX0...17854 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/His.Adl.50.AllAg.Testis.bed ...

  3. Absent testis: does it exist in 46,XY males? | El Gohary | Annals of ...

    African Journals Online (AJOL)

    Conclusion Based on embryological process of testicular development and testicular descent, agentic absent testis cannot exist. Therefore we strongly recommend to re-scope all patients who were initially diagnosed as absent testis to look for a hidden testis at the initial site of development and along the line of descent.

  4. File list: ALL.Emb.20.AllAg.Embryonic_testis [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.Emb.20.AllAg.Embryonic_testis mm9 All antigens Embryo Embryonic testis SRX14917...57137,SRX1156635,SRX149168,SRX149172,SRX1175150,SRX149166,SRX149170,SRX1175149 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.Emb.20.AllAg.Embryonic_testis.bed ...

  5. File list: InP.Emb.10.AllAg.Embryonic_testis [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.Emb.10.AllAg.Embryonic_testis mm9 Input control Embryo Embryonic testis SRX1491...74,SRX149168,SRX957136,SRX149172,SRX149166,SRX1175150,SRX1175149 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.Emb.10.AllAg.Embryonic_testis.bed ...

  6. File list: DNS.Emb.20.AllAg.Embryonic_testis [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.Emb.20.AllAg.Embryonic_testis mm9 DNase-seq Embryo Embryonic testis SRX1156635 ...http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.Emb.20.AllAg.Embryonic_testis.bed ...

  7. Characteristics of A spermatogonia and preleptotene spermatocytes in the vitamin A-deficient rat testis

    NARCIS (Netherlands)

    van Pelt, A. M.; van Dissel-Emiliani, F. M.; Gaemers, I. C.; van der Burg, M. J.; Tanke, H. J.; de rooij, D. G.

    1995-01-01

    The proliferative activity and other characteristics of germ cells in the vitamin A-deficient (VAD) rat testis were investigated. In the VAD testis, A spermatogonia and preleptotene spermatocytes were found. The A spermatogonia in the VAD testis showed a bromodeoxyuridine (BrdU) labeling index of

  8. Experiences with surgical management of undescended testis at a ...

    African Journals Online (AJOL)

    The relative risk of testicular cancer in an undescended testis is between 2.75 to 8 that of a normal male. The risk appears higher in patients with bilateral UDT and late (after age of 12) or uncorrected UDT (David & Iyekoretin,. 2008). Previous studies have estimated that an UDT increases the risk of testicular torsion by 10.

  9. Primary Plasmacytoma of The Testis with no Evidence of Multiple ...

    African Journals Online (AJOL)

    Plasmacytomas of the testis are extremely rare tumours, especially when occurring in the absence of a previous or concurrent diagnosis of multiple myeloma. We report a new case of solitary testicular plasmacytoma, with immunohistochemical studies showing monoclonal cytoplasmic production of IgG lambda light chains, ...

  10. Primary Plasmacytoma of The Testis with no Evidence of Multiple ...

    African Journals Online (AJOL)

    mn

    ABSTRACT. Plasmacytomas of the testis are extremely rare tumours, especially when occurring in the absence of a previous or concurrent diagnosis of multiple myeloma. We report a new case of solitary testicular plasmacytoma, with immunohistochemical studies showing monoclonal cytoplasmic production of IgG lambda ...

  11. Histopathological Effects on Testis of Adult Male Carp, Cyprinus ...

    African Journals Online (AJOL)

    Purpose: To evaluate the estrogenic effect of Bisphenol A (BPA), an endocrine disruptor on the histological features in carp testis. Methods: Adult male fish, koi carp, Cyprinus carpio carpio, were exposed to three graded concentrations of BPA (10, 100 and 1000 μg/L) for a period of 21 days. A single dose of 17-β estradiol (1 ...

  12. Terminology and details of the diagnostic process for testis cancer.

    LENUS (Irish Health Repository)

    Connolly, Stephen S

    2011-03-01

    We examined the process and causes of diagnostic delay, defined as the interval from symptom onset to diagnosis, for testis (germ cell) cancer and the change with time. Diagnostic delay influences disease burden and may be subdivided into symptomatic interval, defined as symptom onset to first presentation, and diagnostic interval, defined as first presentation to diagnosis.

  13. Obestatin induces testosterone secretion from rat testis in vitro ...

    African Journals Online (AJOL)

    In this study, the effect of obestatin (23 amino acid peptide) on testosterone secretion in vitro, in the rat testis was observed. For this purpose, two different doses of obestatin (10-9 M and 10-8 M) were used alone and in combination with human chorionic gonadotropin (hCG) in fasting and fed conditions in two age groups.

  14. Asthenoteratozoospermia in mice lacking testis expressed gene 18 (Tex18)

    NARCIS (Netherlands)

    Jaroszynski, L.; dev, A.; Li, M.; Meinhardt, A.; de rooij, D. G.; Mueller, Christian; Böhm, Detlef; Wolf, S.; Adham, I. M.; Wulf, G.; Engel, W.; Nayernia, K.

    2007-01-01

    Testis expressed gene 18 (Tex18) is a small gene with one exon of 240 bp, which is specifically expressed in male germ cells. The gene encodes for a protein of 80 amino acids with unknown domain. To investigate the function of (Tex18) gene, we generated mice with targeted disruption of the (Tex18)

  15. Synchronous rhabdomyosarcoma of the testis and kidney: A case ...

    African Journals Online (AJOL)

    Babatunde M. Duduyemi

    2015-04-15

    Apr 15, 2015 ... Abstract Rhabdomyosarcoma is the commonest soft tissue sarcoma in both children and adoles- cents representing 40% of such tumours in North America and more than 50% in Africa. The involvement of the paratesticular tissue, testis and the kidney are generally rare and more so when it is occurring ...

  16. Sox9 induces testis development in XX transgenic mice

    NARCIS (Netherlands)

    Vidal, V. P.; Chaboissier, M. C.; de rooij, D. G.; Schedl, A.

    2001-01-01

    Mutations in SOX9 are associated with male-to-female sex reversal in humans. To analyze Sox9 function during sex determination, we ectopically expressed this gene in XX gonads. Here, we show that Sox9 is sufficient to induce testis formation in mice, indicating that it can substitute for the

  17. Steroidogenic Enzyme Histochemistry In The Testis Of Sprague ...

    African Journals Online (AJOL)

    We conclude that the water extract of papaya seed suppresses the activities of steroidogenic enzymes in the testis of Sprague Dawley rats, and that this may contribute to reversible suppression of spermatogenesis, a property that gives a possible male contraceptive potential. Key words: carica papaya seed extract; ...

  18. Obestatin induces testosterone secretion from rat testis in vitro

    African Journals Online (AJOL)

    User

    2011-05-09

    May 9, 2011 ... Obestatin induces testosterone secretion from rat testis in vitro. Hizbullah, Sarwat Jahan* and Shakeel Ahmed. Reproductive physiology lab, Department of Animal sciences Quaid-i-Azam University, Islamabad. Pakistan. Accepted 10 March, 2011. In this study, the effect of obestatin (23 amino acid peptide) ...

  19. Laparoscopic classification of the impalpable testis: an update ...

    African Journals Online (AJOL)

    Purpose We present a classification for the nonpalpable testis (NPT) based on laparoscopic findings and suggest guidelines for the interpretation of these findings. Patients and methods From October 2002 to December 2010, 121 patients with NPT underwent laparoscopy at two tertiary centers of Pediatric surgery in Egypt.

  20. Oxytocin gene expression and action in goat testis.

    Science.gov (United States)

    Inaba, T; Nakayama, Y; Tani, H; Tamada, H; Kawate, N; Sawada, T

    1999-08-01

    Gene expression, immunohistochemical localization, binding and effects of oxytocin (OT) on androgen production in the testis of adult goats were studied. Using polymerase chain reaction (PCR) analysis we were able to detect OT gene transcripts in the goat testis. Immunohistochemistry revealed that both OT and neurophysin epitopes were expressed together in the intratubular regions, especially in the Sertoli cells, suggesting the production of OT in these cells. However, enzyme immunoassay found no difference in OT concentration between testicular arterial and venous plasma. Saturable, specific [3H]-OT binding sites were present in membrane fractions of the goat testis. Scatchard analysis indicated an apparent affinity of 42 +/- 7 L/nmol and binding capacity of 24 +/- 4 fmol OT bound/mg DNA. In vitro treatment of goat testes for 6 h with 100 nM OT led to a 3.5-fold increase (P conversion to DHT. These results indicate that the goat testis produces OT, which may be involved in the local control of androgen biosynthesis.

  1. Efek Mangiferin terhadap Toksisitas Doksorubisin pada Testis Tikus

    Directory of Open Access Journals (Sweden)

    Supraja Dwiyono

    2016-09-01

    Full Text Available Penggunaan doksorubisin (DOX sebagai antikanker dapat menyebabkan efek samping di organ lainseperti testis akibat peningkatan stres oksidatif. Penelitian ini bertujuan untuk mengetahui efek antioksidanmangiferin yang terkandung dalam Mangifera indica untuk mengurangi toksisitas testis. Sebanyak 24 ekortikus jantan sprague dawley, dibagi menjadi empat kelompok. Kelompok normal mendapatkan injeksi NaCl0,9%, kelompok kontrol negatif mendapatkan DOX total 15mg/kgBB; kelompok DOX mendapat mangiferin30mg/kgBB dan mangiferin 60mg/kgBB. Setelah 7 minggu, tikus dimatikan dan testis dikumpulkan untukanalisis parameter stres oksidatif biokimia yaitu kadar malonedyaldehide (MDA, aktivitas superoxidedysmutase (SOD, perubahan histologi dan apoptosis kaspase-9 dan kaspase-12. Pemberian mangiferindosis 30mg/kgBB dan 60mg/kgBB selama 7 minggu dapat mengurangi kerusakan sel spermatogenik dansel sertoli tubulus seminiferus testis, menurunkan kadar MDA dan menurunkan ekspresi kaspase-9 padakelompok yang diberikan DOX dan mangiferin. Perbaikan parameter tersebut mengindikasikan mangiferinmempunyai efek proteksi terhadap kerusakan sel spematogenik dan sel sertoli tubulus seminiferus testis tikusyang diberikan DOX. Kata kunci: toksisitas doksorubisin, stres oksidatif, apoptosis, mangiferin, testis.   Mangiferin Effects against Doxorubicin Toxicity in Rat Testis Abstract The use of doxorubicin (DOX as an anti-cancer agent might cause side effects to other organs suchas testicle due to increased oxidative stress. This study was aimed to investigate the antioxidant effect ofmangiferin inside Mangifera indica for lowering testicle toxicity. A total of 24 sprague dawley male rats wasdivided into 4 groups. The normal group was injected with 0.9% of NaCl, the negative control group receivedDOX with dosage of 15 mg/BW while DOX treated group received mangiferin with dosage of 30 mg/BW and60 mg/BW. After 7 weeks, all rats were killed and testicles from every rats were

  2. Pengaruh Perbedaan Dosis Ekstrak Biji Jarak Pagar (Jatropha curcas terhadap Jumlah Spermatozoa, Spermatozoa Motil, Berat Testis, dan Diameter Testis pada Mencit Jantan (Mus Musculus

    Directory of Open Access Journals (Sweden)

    Haifa Wahyu

    2016-08-01

    Full Text Available AbstrakIndonesia menghadapi persoalan kependudukan dan keluarga berencana yang cukup berat, salah satu penyebabnya dikarenakan rendahnya partisipasi pria dalam penggunaan kontrasepsi. Salah satu tanaman yang bersifat antifertilitas adalah jarak pagar (Jatropha curcas. Penelitian ini bertujuan mengetahui pengaruh perbedaan dosis ekstrak biji jarak pagar terhadap jumlah spermatozoa, spermatozoa motil, berat testis dan diameter testis pada mencit jantan (Mus musculus. Penelitian ini menggunakan metode post test only control group design terhadap mencit jantan dengan berat 20–30 gram. Sampel terdiri dari 24 ekor mencit dibagi dalam 3 kelompok P1, P2, dan P3 dengan memberikan ekstrak biji jarak pagar dengan 3 tingkatan dosis selama 36 hari. Hasil penelitian semakin tinggi dosis yang diberikan semakin rendah jumlah spermatozoa, spermatozoa motil, berat testis dan diameter testis namun secara statistik tidak terdapat pengaruh yang bermakna pada jumlah spermatozoa p=0,06 dan spermatozoa motil p=0,15 (p > 0,05 dan terdapat pengaruh bermakna pada berat testis p=0,00 dan diameter testis p= 0,00 (p< 0,05.Kesimpulannya pemberian ekstrak biji jarak pagar menurunkan jumlah spermatozoa, spermatozoa motil, berat testis dan diameter testis namun secara statistik ekstrak biji jarak pagar tidak terdapat pengaruh yang bermakna terhadap jumlah spermatozoa dan spermatozoa motil dan terdapat pengaruh bermakna terhadap berat testis dan diameter testis.Kata kunci: ekstrak biji jarak pagar, jumlah spermatozoa, spermatozoa motil, berat testis, diameter testis. AbstractIndonesia faces the quite severe problem of population and family planning, one reason is because the low participation of men in the use of contraception. One antifertility plants and are found in Indonesia is jatropha (Jatropha curcas. This study aims to determine the effect of different doses of extract of Jatropha seeds on sperm count, motile of sperm, testis weight and diameter of the testis in male

  3. Xenoestrogens diethylstilbestrol and zearalenone negatively influence pubertal rat's testis.

    Science.gov (United States)

    Filipiak, Eliza; Walczak-Jedrzejowska, Renata; Oszukowska, Elzbieta; Guminska, Anna; Marchlewska, Katarzyna; Kula, Krzysztof; Slowikowska-Hilczer, Jolanta

    2009-01-01

    The aim of this study was to assess the impact of xenoestrogens: diethylstilbestrol (DES) and zearalenone (ZEA) on rat's pubertal testis and to compare it with the effect of natural estrogen - 17beta-estradiol (E). Male Wistar rats were daily, subcutaneously injected at 5th-15th postnatal days (p.d.) with E (1.25 or 12.5 mug) or DES (1.25 or 12.5 mug) or ZEA (4 or 40 mug) or vehicle. At 16th p.d. testes were dissected, weighted, and paraffin embedded. Following parameters were assessed: diameter and length of seminiferous tubule, numbers of spermatogonia A+intermediate+B (A/In/B), preleptotene spermatocytes (PL), leptotene+zygotene+pachytene spermatocytes (L/Z/PA) and Sertoli cells per testis. Testes weight, seminiferous tubule diameter and length were decreased by both doses of E, DES and ZEA. DES effect was the strongest, but its influence on testis weight and seminiferous tubule length, on the contrary to E and ZEA, was not dose-dependent. Similarly, DES in both doses had the most severe negative impact on the number of germ and Sertoli cells. The negative influence of E on germ cells was less pronounced. The negative effect of ZEA was seen only after administration of the higher dose on spermatogonia number, while DES and E decreased A/In/B number more evidently. Sertoli cell number were decreased after both doses of E. ZEA40 decreased Sertoli cell number while ZEA4 had no effect. exposure of prepubertal male rat to DES has the strongest detrimental effect on the developing testis in comparison to E and ZEA. Both, E and DES, decreased number of germ and Sertoli cells, diminished seminiferous tubule diameter, length and testis weight. ZEA had much more weaker effect than the potent estrogens.

  4. Xenoestrogens diethylstilbestrol and zearalenone negatively influence pubertal rat's testis.

    Directory of Open Access Journals (Sweden)

    Katarzyna Marchlewska

    2010-01-01

    Full Text Available The aim of this study was to assess the impact of xenoestrogens: diethylstilbestrol (DES and zearalenone (ZEA on rat's pubertal testis and to compare it with the effect of natural estrogen - 17beta-estradiol (E. Male Wistar rats were daily, subcutaneously injected at 5th-15th postnatal days (p.d. with E (1.25 or 12.5 mug or DES (1.25 or 12.5 mug or ZEA (4 or 40 mug or vehicle. At 16th p.d. testes were dissected, weighted, and paraffin embedded. Following parameters were assessed: diameter and length of seminiferous tubule, numbers of spermatogonia A+intermediate+B (A/In/B, preleptotene spermatocytes (PL, leptotene+zygotene+pachytene spermatocytes (L/Z/PA and Sertoli cells per testis. Testes weight, seminiferous tubule diameter and length were decreased by both doses of E, DES and ZEA. DES effect was the strongest, but its influence on testis weight and seminiferous tubule length, on the contrary to E and ZEA, was not dose-dependent. Similarly, DES in both doses had the most severe negative impact on the number of germ and Sertoli cells. The negative influence of E on germ cells was less pronounced. The negative effect of ZEA was seen only after administration of the higher dose on spermatogonia number, while DES and E decreased A/In/B number more evidently. Sertoli cell number were decreased after both doses of E. ZEA40 decreased Sertoli cell number while ZEA4 had no effect. Conclusion: exposure of prepubertal male rat to DES has the strongest detrimental effect on the developing testis in comparison to E and ZEA. Both, E and DES, decreased number of germ and Sertoli cells, diminished seminiferous tubule diameter, length and testis weight. ZEA had much more weaker effect than the potent estrogens.

  5. Assessment of the Hewlett-Packard HP47210A capnometer.

    Science.gov (United States)

    Kinsella, S M

    1985-09-01

    The Hewlett-Packard HP47210A Capnometer, a "mainstream" infra-red carbon dioxide analyser, has a sensor in-line with the patient circuit. The calibration apparatus is included within the unit, and an internal microprocessor corrects for the presence of nitrous oxide and varying concentrations of oxygen. Maximum error was 0.37 kPa for carbon dioxide partial pressures less than 5.3 kPa, and less than 3.6% for partial pressures of carbon dioxide greater than 5.3 kPa.

  6. Structural effects at Λ3Hp-scattering

    International Nuclear Information System (INIS)

    Tartakovskij, V.K.; Fursaev, A.V.; Ivanova, O.I.

    2003-01-01

    In the paper the calculated dependences of falling hyper-tritons energy E=156 MeV differential cross sections from the scattering angle in the center of mass system θ and for more high energy values E from coupling energy ε 0 of Λ 3 H with regard to its disintegration into Λ-hyperon and deuteron are presented. It is established the strong dependence of differential cross section from ε 0 , moreover the sensitivity of differential cross sections to ε 0 value grows up with increasing of energy E. This allows to use the Λ 3 Hp-scattering as a method for ε 0 value elaboration

  7. Spectral/hp element methods: Recent developments, applications, and perspectives

    DEFF Research Database (Denmark)

    Xu, Hui; Cantwell, Chris; Monteserin, Carlos

    2018-01-01

    The spectral/hp element method combines the geometric flexibility of the classical h-type finite element technique with the desirable numerical properties of spectral methods, employing high-degree piecewise polynomial basis functions on coarse finite element-type meshes. The spatial approximation...... regularity assumptions an exponential reduction in approximation error between numerical and exact solutions can be achieved. This method has now been applied in many simulation studies of both fundamental and practical engineering flows. This paper briefly describes the formulation of the spectral...... method in more complex science and engineering applications are discussed....

  8. SGN III code conversion from Apollo to HP

    International Nuclear Information System (INIS)

    Lee, Hae Cho

    1996-04-01

    SGN III computer code is used to analyze transient behavior of reactor coolant system, pressurizer and steam generators in the event of main steam line break (MSLB), and to calculate mass and energy release for containment design. This report firstly describes detailed work carried out for installation of SFN III on Apollo DN 10000 and code validation results after installation. Secondly, a series of work is also describes in relation to installation of SGN III on HP 9000/700 series as well as relevant code validation results. Attached is a report on software verification and validation results. 8 refs. (Author) .new

  9. CESEC III code conversion from Apollo to HP9000

    International Nuclear Information System (INIS)

    Lee, Hae Cho

    1996-01-01

    CESEC code is a computer program used to analyze transient behaviour of reactor coolant systems of nuclear power plants. CESEC III is an extension of original CESEC code in order to apply wide range of accident analysis including ATWS model. Major parameters during the transients are calculated by CESEC. This report firstly describes detailed work carried out for installation of CESEC III on Apollo DN10000 and code validation results after installation. Secondly, A series of work is also described in relation to installation of CESECIII on HP 9000/700 series as well as relevant code validation results. Attached is a report on software verification and validation results. 7 refs. (Author) .new

  10. CONTRANS 2 code conversion from Apollo to HP

    International Nuclear Information System (INIS)

    Lee, Hae Cho

    1996-01-01

    CONTRANS2 computer code is used to calculate transient thermal hydraulic responses of containment building to loss of coolant and main steam line break accident. Mass and energy release to the containment following an accident are code inputs. This report firstly describes detailed work carried out for installation of CONTRANS2 on Apollo DN10000 and code validation results after installation. Secondly, A series of work is also describes in relation to installation of CONTRANS2 on HP 9000/700 series as well as relevant code validation results. Attached is a report on software verification and validation results. 7 refs. (Author) .new

  11. Epigenetic aspects of HP1 exchange kinetics in apoptotic chromatin

    Czech Academy of Sciences Publication Activity Database

    Legartová, Soňa; Jugová, Alžbeta; Stixová, Lenka; Kozubek, Stanislav; Fojtová, Miloslava; Zdráhal, Z.; Lochmanová, G.; Bártová, Eva

    2013-01-01

    Roč. 95, č. 2 (2013), s. 167-179 ISSN 0300-9084 R&D Projects: GA ČR(CZ) GAP302/10/1022; GA ČR(CZ) GBP302/12/G157; GA MŠk(CZ) LD11020; GA MŠk(CZ) ED1.1.00/02.0068 Institutional research plan: CEZ:AV0Z50040702 Institutional support: RVO:68081707 Keywords : Apoptosis * Histones * HP1 Subject RIV: BO - Biophysics Impact factor: 3.123, year: 2013

  12. PULSION registered HP: Tunable, High Productivity Plasma Doping

    International Nuclear Information System (INIS)

    Felch, S. B.; Torregrosa, F.; Etienne, H.; Spiegel, Y.; Roux, L.; Turnbaugh, D.

    2011-01-01

    Plasma doping has been explored for many implant applications for over two decades and is now being used in semiconductor manufacturing for two applications: DRAM polysilicon counter-doping and contact doping. The PULSION HP is a new plasma doping tool developed by Ion Beam Services for high-volume production that enables customer control of the dominant mechanism--deposition, implant, or etch. The key features of this tool are a proprietary, remote RF plasma source that enables a high density plasma with low chamber pressure, resulting in a wide process space, and special chamber and wafer electrode designs that optimize doping uniformity.

  13. SGN III code conversion from Apollo to HP

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Hae Cho [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

    1996-04-01

    SGN III computer code is used to analyze transient behavior of reactor coolant system, pressurizer and steam generators in the event of main steam line break (MSLB), and to calculate mass and energy release for containment design. This report firstly describes detailed work carried out for installation of SFN III on Apollo DN 10000 and code validation results after installation. Secondly, a series of work is also describes in relation to installation of SGN III on HP 9000/700 series as well as relevant code validation results. Attached is a report on software verification and validation results. 8 refs. (Author) .new.

  14. Targeted identification of glycosylated proteins in the gastric pathogen Helicobacter pylori (Hp).

    Science.gov (United States)

    Champasa, Kanokwan; Longwell, Scott A; Eldridge, Aimee M; Stemmler, Elizabeth A; Dube, Danielle H

    2013-09-01

    Virulence of the gastric pathogen Helicobacter pylori (Hp) is directly linked to the pathogen's ability to glycosylate proteins; for example, Hp flagellin proteins are heavily glycosylated with the unusual nine-carbon sugar pseudaminic acid, and this modification is absolutely essential for Hp to synthesize functional flagella and colonize the host's stomach. Although Hp's glycans are linked to pathogenesis, Hp's glycome remains poorly understood; only the two flagellin glycoproteins have been firmly characterized in Hp. Evidence from our laboratory suggests that Hp synthesizes a large number of as-yet unidentified glycoproteins. Here we set out to discover Hp's glycoproteins by coupling glycan metabolic labeling with mass spectrometry analysis. An assessment of the subcellular distribution of azide-labeled proteins by Western blot analysis indicated that glycoproteins are present throughout Hp and may therefore serve diverse functions. To identify these species, Hp's azide-labeled glycoproteins were tagged via Staudinger ligation, enriched by tandem affinity chromatography, and analyzed by multidimensional protein identification technology. Direct comparison of enriched azide-labeled glycoproteins with a mock-enriched control by both SDS-PAGE and mass spectrometry-based analyses confirmed the selective enrichment of azide-labeled glycoproteins. We identified 125 candidate glycoproteins with diverse biological functions, including those linked with pathogenesis. Mass spectrometry analyses of enriched azide-labeled glycoproteins before and after cleavage of O-linked glycans revealed the presence of Staudinger ligation-glycan adducts in samples only after beta-elimination, confirming the synthesis of O-linked glycoproteins in Hp. Finally, the secreted colonization factors urease alpha and urease beta were biochemically validated as glycosylated proteins via Western blot analysis as well as by mass spectrometry analysis of cleaved glycan products. These data set the

  15. HELOKA-HP thermal-hydraulic model validation and calibration

    Energy Technology Data Exchange (ETDEWEB)

    Jin, Xue Zhou; Ghidersa, Bradut-Eugen; Badea, Aurelian Florin

    2016-11-01

    Highlights: • The electrical heater in HELOKA-HP has been modeled with RELAP5-3D using experimental data as input. • The model has been validated using novel techniques for assimilating experimental data and the representative model parameters with BEST-EST. • The methodology is successfully used for reducing the model uncertainties and provides a quantitative measure of the consistency between the experimental data and the model. - Abstract: The Helium Loop Karlsruhe High Pressure (HELOKA-HP) is an experimental facility for the testing of various helium-cooled components at high temperature (500 °C) and high pressure (8 MPa) for nuclear fusion applications. For modeling the loop thermal dynamics, a thermal-hydraulic model has been created using the system code RELAP5-3D. Recently, new experimental data covering the behavior of the loop components under relevant operational conditions have been made available giving the possibility of validating and calibrating the existing models in order to reduce the uncertainties of the simulated responses. This paper presents an example where such process has been applied for the HELOKA electrical heater model. Using novel techniques for assimilating experimental data, implemented in the computational module BEST-EST, the representative parameters of the model have been calibrated.

  16. The role of MRI and laparoscopy in the management of the nonpalpable testis

    Energy Technology Data Exchange (ETDEWEB)

    Nishizawa, Shuji; Nukui, Akinori; Koshimizu, Takeshi [Jichi Medical School, Minamikawachi, Tochigi (Japan)] (and others)

    2002-12-01

    Preoperative localization of nonpalpable testes will aid in planning of the surgical management. We evaluate the efficacy of magnetic resonance imaging (MRI) as the preoperative study in the management of the nonpalpable testis in the laparoscopic era. From 1997 to 2001, a total of 30 MRI evaluation was performed for 30 patients with nonpalpable testis. When the imaging study demonstrated a viable testis in the inguinal region, the patient underwent inguinal orchiopexy. Diagnostic laparoscopy was performed for the patient whose testis was intraabdominal or could not be detected by imaging. Thirty-one testes from 30 patients were evaluated. The imaging studies showed 16 testes from 15 patients in the inguinal region. Fifteen testes were found in the inguinal region and one was intraabdominal during groin exploration. Four testes were pointed out to be intraabdominal by MRI and identified by laparoscopic examination. Eleven testes could not be detected by the imaging studies and they underwent laparoscopic examination. Two abdominal testes were identified. Nine patients underwent inguinal surgical exploration and had a diagnosis of inguinal vanishing testis. No gonadal tissue was identified in those specimens. The sensitivity and the specificity of our MRI studies in predicting the presence of nonpalpable testis were 90.5% and 100%, respectively. Inguinal undescended testes could be diagnosed accurately with MRI and laparoscopy was informative regarding the testis location in all cases. When no intraabdominal testis is identified laparoscopically in the patient whose testis can not be detected in the inguinal region by MRI, the testis is likely vanished. (author)

  17. Dynamics of testis-ova in a wild population of Japanese pond frogs, Rana nigromaculata.

    Science.gov (United States)

    Kobayashi, Tohru; Kumakura, Masahiko; Yoshie, Sumio; Sugishima, Tomomi; Horie, Yoshifumi

    2015-02-01

    Although many studies have reported the occurrence of testis-ova in wild frog populations, the origin and trigger of testis-ova differentiation/development remain unclear. A high frequency of testis-ova has been previously reported for wild populations of the Japanese pond frog, Rana nigromaculata (cf. Iwasawa and Asai, '59). In the present study, we aimed to clarify the dynamics of testis-ova in this frog species, including the origin and artificial induction of testis-ova. Testis-ova were observed in both mature frogs and puberty-stage frogs (i.e., 0- and 1-year-old frogs). However, the early stages of testis-ova (~pachytene stage) were mostly observed in puberty-stage male frogs at the onset of spermatogenesis. The early stages of testis-ova were observed in the cysts of early secondary spermatogonia and the single cysts of the primary spermatogonium. This finding indicates that testis-ova differentiation occurs during spermatogonial proliferation and that it is correlated with the initiation of spermatogenesis. We also examined whether estrogen exposure induced testis-ova differentiation and how it is correlated with the progression of spermatogenesis. When 1-year-old frogs were exposed to estradiol-17β during spring (i.e., when spermatogenesis was initiated), testis-ova differentiation was induced in a dose-dependent manner. However, this phenomenon did not occur in 1-year-old frogs during summer, (i.e., when the transition from spermatogonia to spermatocytes mainly occurs). These results present the first evidence that testis-ova of the Japanese pond frog are derived from primary and early secondary spermatogonia, and that estrogen exposure induces testis-ova differentiation accompanied by the initiation of spermatogenesis. © 2015 Wiley Periodicals, Inc.

  18. Recurring exon deletions in the HP (haptoglobin) gene contribute to lower blood cholesterol levels.

    Science.gov (United States)

    Boettger, Linda M; Salem, Rany M; Handsaker, Robert E; Peloso, Gina M; Kathiresan, Sekar; Hirschhorn, Joel N; McCarroll, Steven A

    2016-04-01

    One of the first protein polymorphisms identified in humans involves the abundant blood protein haptoglobin. Two exons of the HP gene (encoding haptoglobin) exhibit copy number variation that affects HP protein structure and multimerization. The evolutionary origins and medical relevance of this polymorphism have been uncertain. Here we show that this variation has likely arisen from many recurring deletions, more specifically, reversions of an ancient hominin-specific duplication of these exons. Although this polymorphism has been largely invisible to genome-wide genetic studies thus far, we describe a way to analyze it by imputation from SNP haplotypes and find among 22,288 individuals that these HP exonic deletions associate with reduced LDL and total cholesterol levels. We further show that these deletions, and a SNP that affects HP expression, appear to drive the strong association of cholesterol levels with SNPs near HP. Recurring exonic deletions in HP likely enhance human health by lowering cholesterol levels in the blood.

  19. HELICOBACTER PYLORI (HP) INFECTION IN OBESE PATIENTS UNDERGOING ROUXEN- Y GASTRIC BYPASS; EFFICACY OF TWO DIFFERENT TREATMENT REGIMENS IN HP ERADICATION.

    Science.gov (United States)

    Cuesta Hernández, Martín; Pérez Peña, Celia; Matía Martín, Pilar; Cabrerizo García, Lucio; Pérez-Ferre, Natalia; Sánchez-Pernaute, Andrés; Torres García, Antonio; Rubio Herrera, Miguel Ángel

    2015-08-01

    the ultimate cause for the increased incidence of gastric ulcer following Roux-en-Y gastric bypass (RYGB) remains unclear. Treatment of HP infection is recommended before surgery in countries with high prevalence such as Spain in other to diminish the risk. However, the current regimens used might not be adequate in view of the high failure rate for HP eradication. we reviewed 243 patients retrospectively undergoing RYGB and found 111 patients (45%) with HP infection. Therefore, we compared the eradication rate between 2 different regimens. 70 patients received OCA(Omeprazole:20 mg/12h, Clarithromycin 500 mg/12h and Amoxicillin 1 gram/12h for 10 days) while 41 patients received OLA (Omeprazole 20 mg/12 hours, Levofloxacin 500 mg/12hours and Amoxicillin 1 gram/12h for 10 days) for HP eradication. In 56/70 (80%) patients receiving OCA therapy HP was eradicated compared to 37/41 (91%) receiving OLA as first line therapy (p = 0.283). When used as second line therapy, in 13/14 (92%) patients receiving OLA HP was eradicated. clarithromycin resistance remains a matter of concern in this population while OLA seems to be a good alternative therapy for HP eradication, especially when OCA regimen fails. Copyright AULA MEDICA EDICIONES 2014. Published by AULA MEDICA. All rights reserved.

  20. Sex Cord-Gonadal Stromal Tumor of the Rete Testis

    Directory of Open Access Journals (Sweden)

    Kamran P. Sajadi

    2009-01-01

    Full Text Available A 34-year-old tetraplegic patient with suppurative epididymitis was found on follow-up examination and ultrasonography to have a testicular mass. The radical orchiectomy specimen contained an undifferentiated spindled sex cord-stromal tumor arising in the rete testis. Testicular sex cord-stromal tumors are far less common than germ cell neoplasms and are usually benign. The close relationship between sex cords and ductules of the rete testis during development provides the opportunity for these uncommon tumors to arise anatomically within the rete tesis. This undifferentiated sex cord-stromal tumor, occurring in a previously unreported location, is an example of an unusual lesion mimicking an intratesticular malignant neoplasm.

  1. Gene expression during testis development in Duroc boars

    DEFF Research Database (Denmark)

    Lervik, Siri; Kristoffersen, Anja Bråthen; Conley, Lene

    2015-01-01

    and rodents, act at specific ages and developmental stages in the boar testis. The age dependency and complexity shown for development-specific testis transcripts must be considered when selecting phenotypic parameters for genetic selection for low androstenone. The results of selection based on measurement......Androstenone is a steroid pheromone occurring in the pubertal Leydig cells. Breeding against androstenone can decrease pheromone odour in swine meat but appears to cause unwanted side effects such as delayed onset of puberty. To study causality, global gene expression in developing boar testes...... with the onset of pubertal development. With elevated steroidogenesis (weeks 16 to 27), there was an increase in the expression of genes in the MAPK pathway, STAR and its analogue STARD6. A pubertal shift in genes coding for cellular cholesterol transport was observed. Increased expression of meiotic pathways...

  2. Engineering of Helicobacter pylori dimeric oxidoreductase DsbK (HP0231

    Directory of Open Access Journals (Sweden)

    Katarzyna Marta Bocian-Ostrzycka

    2016-07-01

    Full Text Available The formation of disulfide bonds that are catalyzed by proteins of the Dsb (disulfide bond family is crucial for the correct folding of many extracytoplasmic proteins. Thus, this formation plays an essential, pivotal role in the assembly of many virulence factors. The Helicobacter pylori disulfide bond-forming system is uncomplicated compared to the best-characterized Escherichia coli Dsb pathways. It possesses only two extracytoplasmic Dsb proteins named HP0377 and HP0231. As previously shown, HP0377 is a reductase involved in the process of cytochrome c maturation. Additionally, it also possesses disulfide isomerase activity. HP0231 was the first periplasmic dimeric oxidoreductase involved in disulfide generation to be described. Although HP0231 function is critical for oxidative protein folding, its structure resembles that of dimeric EcDsbG, which does not confer this activity. However, the HP0231 catalytic motifs (CXXC and the so-called cis-Pro loop are identical to that of monomeric EcDsbA. To understand the functioning of HP0231, we decided to study the relations between its sequence, structure and activity through an extensive analysis of various HP0231 point mutants, using in vivo and in vitro strategies. Our work shows the crucial role of the cis-Pro loop, as changing valine to threonine in this motif completely abolishes the protein function in vivo. Functioning of HP0231 is conditioned by the combination of CXXC and the cis-Pro loop, as replacing the HP0231 CXXC motif by the motif from EcDsbG or EcDsbC results in bifunctional protein, at least in E. coli. We also showed that the dimerization domain of HP0231 ensures contact with its substrates. Moreover, the activity of this oxidase is independent on the structure of the catalytic domain. Finally, we showed that HP0231 chaperone activity is independent of its redox function.

  3. Frequency of helicobacter pylori (hp) infection in children with recurrent abdominal pain (rap)

    International Nuclear Information System (INIS)

    Mahmud, S.; Ali, S.

    2015-01-01

    To study the frequency of Helicobacter Pylori (HP) infection among children with recurrent abdominal pain (RAP) Study Design: Cross-sectional comparative study. Place and Duration of Study: Military Hospital (MH), Rawalpindi from December 2011 to February 2012. Patients and Methods: One hundred children of either gender aged 2 to 12 years presenting with RAP were tested for HP at Paediatric OPD MH, Rawalpindi who consented to participate in the study. Those children who tested positive for Helicobacter Pylori Stool Antigen Test (HPSAT) were labeled as those having Hp infection. The stool assay was performed using the HpSAT kit and the socio-demographic and clinical profiles of children were associated. Results: Out of 100 children included in the study HpSAT was positive in 38% children. Frequency of Hp infection was significantly associated with source of drinking water (p = 0.014), socioeconomic status (p = 0.001) and positive family history of yspepsia (p= 0.023). While age and gender have no significant association with HP infection. Conclusion: Hp infection is very common in children presenting with RAP in our Paediatric OPD. Children with family history of dyspepsia, from low socioeconomic class and those drinking filtered water are at greater risk for HP infection. It is recommended that children from other populations in our country should also be tested in their medical health facilities in order to have a wider analysis of this problem in our setup. (author)

  4. A nonlinear HP-type complementary resistive switch

    Directory of Open Access Journals (Sweden)

    Paul K. Radtke

    2016-05-01

    Full Text Available Resistive Switching (RS is the change in resistance of a dielectric under the influence of an external current or electric field. This change is non-volatile, and the basis of both the memristor and resistive random access memory. In the latter, high integration densities favor the anti-serial combination of two RS-elements to a single cell, termed the complementary resistive switch (CRS. Motivated by the irregular shape of the filament protruding into the device, we suggest a nonlinearity in the resistance-interpolation function, characterized by a single parameter p. Thereby the original HP-memristor is expanded upon. We numerically simulate and analytically solve this model. Further, the nonlinearity allows for its application to the CRS.

  5. Modification of BINX code for HP9000 system

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Y. C.; Kim, Y. J.; Kim, Y. G.; Chung, H. T.

    1997-12-01

    As one of the efforts to construct an integrated computation system, the K-CORE system for LMR core design and analysis, the BINX code which converts format of CCCC standard input/output files has been modified so that it works on HP 9000 workstations. The BINX code was improved to manipulate input/output files in the newer CCCC version IV format and some bugs in the former code were eliminated. These give BINX the compatibility of the input/output files among calculation modules. Hence the cross-section library processing system that can convert and produce standard input/output files satisfying the user`s function requirement has been established in the K-CORE system. (author). 10 refs.

  6. HP Memristor mathematical model for periodic signals and DC

    KAUST Repository

    Radwan, Ahmed G.

    2012-07-28

    In this paper mathematical models of the HP Memristor for DC and periodic signal inputs are provided. The need for a rigid model for the Memristor using conventional current and voltage quantities is essential for the development of many promising Memristors\\' applications. Unlike the previous works, which focuses on the sinusoidal input waveform, we derived rules for any periodic signals in general in terms of voltage and current. Square and triangle waveforms are studied explicitly, extending the formulas for any general square wave. The limiting conditions for saturation are also provided in case of either DC or periodic signals. The derived equations are compared to the SPICE model of the Memristor showing a perfect match.

  7. Effects of a simulated microgravity model on cell structure and function in rat testis and epididymis

    Science.gov (United States)

    Hadley, Jill A.; Hall, Joseph C.; O'Brien, Ami; Ball, Richard

    1992-01-01

    The effect of simulated microgravity on the structure and function of the testis and epididymis cells was investigated in rats subjected to 7 days of tail suspension. Results of a histological examination revealed presence of disorganized seminiferous tubules and accumulation of large multinucleated cells and spermatids in the lumen of the epididymis. In addition, decreases in the content of testis protein and in testosterone levels in the testis, the interstitial fluid, and the epididymis were observed.

  8. HP-β-cyclodextrin as an inhibitor of amyloid-β aggregation and toxicity.

    Science.gov (United States)

    Ren, Baiping; Jiang, Binbo; Hu, Rundong; Zhang, Mingzhen; Chen, Hong; Ma, Jie; Sun, Yan; Jia, Lingyun; Zheng, Jie

    2016-07-27

    Amyloid deposits of misfolded amyloid-β protein (Aβ) on neuronal cells are a pathological hallmark of Alzheimer's disease (AD). Prevention of the abnormal Aβ aggregation has been considered as a promising therapeutic strategy for AD treatment. To prevent reinventing the wheel, we proposed to search the existing drug database for other diseases to identify potential Aβ inhibitors. Herein, we reported the inhibitory activity of HP-β-cyclodextrin (HP-β-CD), a well-known sugar used in drug delivery, genetic vector, environmental protection and treatment of Niemann-Pick disease type C1 (NPC1), against Aβ1-42 aggregation and Aβ-induced toxicity, with the aim of adding a new function as a sugar-based Aβ inhibitor. Experimental data showed that HP-β-CD molecules were not only nontoxic to cells, but also greatly inhibited Aβ fibrillization and reduced Aβ-induced toxicity in a concentration-dependent manner. At an optimal molar ratio of Aβ : HP-β-CD = 1 : 2, HP-β-CD enabled the reduction of 60% of Aβ fibrils and increased the cell viability to 92%. Such concentration-dependent inhibitor capacity of HP-β-CD was likely attributed to several combined effects, including the enhancement of Aβ-HP-β-CD interactions, prevention of structural transition of Aβ peptides towards β-sheet structures, and reduction of self-aggregation of HP-β-CD. In parallel, molecular simulations further revealed the atomic details of HP-β-CD interacting with the Aβ oligomer, showing that HP-β-CD had a high tendency to interact with hydrophobic residues of Aβ in two β-strands and the N-terminal tail. More importantly, we identified that the inner hydrophobic cavity of HP-β-CD was a key active site for Aβ inhibition. Once the inner cavity of HP-β-CD was blocked by a small hydrophobic molecule of ferulic acid, HP-β-CD completely lost its inhibition capacity against Aβ. Given the already established pharmaceutical functions of HP-β-CD in drug delivery, our findings

  9. Could be a link between non atopic asthma and HP infection?

    Science.gov (United States)

    Pedullà, M; Perrone, L; Fierro, V; Capristo, C; Salpietro, C; Leonardi, S; La Rosa, M; Arrigo, T; Licari, A; Longaretti, P; Miraglia Del Giudice, M

    2012-01-01

    A potential role of Helicobater Pylori (HP) infection in several extra-intestinal pathologies has been recently suggested. The aim of our study was to assess the role of serology positive for HP in atopic and non atopic infants and children affected by atopic dermatitis, urticaria, rhinitis and asthma. We included 615 children affected by atopic diseases. According to prick test positivity and age, we divided the patients into two groups: atopic or non-atopic patients and infants (0-2 years) or children (2-12 years). The serum levels of antibodies for H. pylori immunoglobulin G were measured by using an ELISA test. We found a not significant difference between group 1 and group 2 about atopy. There was a significant higher frequency of HP positive serology in older children. As for infants, a higher significant prevalence of HP positive serology was found in non-atopic patients. HP positive serology was significantly higher only in non-atopic infants affected by atopic dermatitis and urticaria than in atopic. In group 2, non atopic children shown a significant increase in the prevalence of HP serum positivity than atopic children. As for asthma, there was an higher prevalence of HP serology positive in non atopic asthmatic children group than in atopic asthmatics. On the contrary, the prevalence of positive HP serology was not significantly different between atopic and non atopic children affected by dermatitis, urticaria, and rhinitis. The present data confirm an inverse association between HP positive serology and atopy in both groups. However, the higher prevalence of positive HP serology was observed in non atopic asthmatics children than in atopic asthmatics. We could speculate that HP infection can favour non-atopic asthma onset.

  10. Interaction of HP1 and Brg1/Brm with the globular domain of histone H3 is required for HP1-mediated repression.

    Directory of Open Access Journals (Sweden)

    Marc Lavigne

    2009-12-01

    Full Text Available The heterochromatin-enriched HP1 proteins play a critical role in regulation of transcription. These proteins contain two related domains known as the chromo- and the chromoshadow-domain. The chromo-domain binds histone H3 tails methylated on lysine 9. However, in vivo and in vitro experiments have shown that the affinity of HP1 proteins to native methylated chromatin is relatively poor and that the opening of chromatin occurring during DNA replication facilitates their binding to nucleosomes. These observations prompted us to investigate whether HP1 proteins have additional histone binding activities, envisioning also affinity for regions potentially occluded by the nucleosome structure. We find that the chromoshadow-domain interacts with histone H3 in a region located partially inside the nucleosomal barrel at the entry/exit point of the nucleosome. Interestingly, this region is also contacted by the catalytic subunits of the human SWI/SNF complex. In vitro, efficient SWI/SNF remodeling requires this contact and is inhibited in the presence of HP1 proteins. The antagonism between SWI/SNF and HP1 proteins is also observed in vivo on a series of interferon-regulated genes. Finally, we show that SWI/SNF activity favors loading of HP1 proteins to chromatin both in vivo and in vitro. Altogether, our data suggest that HP1 chromoshadow-domains can benefit from the opening of nucleosomal structures to bind chromatin and that HP1 proteins use this property to detect and arrest unwanted chromatin remodeling.

  11. Distribution of highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) in different stages of gestation sows: HP-PRRSV distribution in gestation sows.

    Science.gov (United States)

    Wang, Xiaofei; Qiu, Hongkai; Zhang, Minxia; Cai, Xinna; Qu, Yajin; Hu, Dongfang; Zhao, Xiaomin; Zhou, Enmin; Liu, Sidang; Xiao, Yihong

    2015-08-15

    Highly pathogenic PRRSV (HP-PRRSV) emerged in China in 2006 and caused severe reproductive losses, particularly in late-term sows. To determine whether these reproductive failures were related to the susceptibility of late-term sows to HP-PRRV, 60- and 90-days of gestation sows were infected with HP-PRRSV isolate TA-12 (GenBank accession HQ417620). A monoclonal antibody specific to the C-terminal of the nucleocapsid protein was used to evaluate viral distribution by IHC. This showed that HP-PRRSV had a similar distribution in both sets of sows. However, HP-PRRSV infection led to dramatically decreased serum levels of luteinizing hormone (LH) and 17-β-estradiol (E2) in late-term sows, while only E2 was decreased in the 60-day sows. These results indicate that HP-PRRSV-induced reproductive failure is more likely due to reproductive hormone level imbalances rather than tissue tropism differences. Copyright © 2015 Elsevier B.V. All rights reserved.

  12. 78 FR 48468 - Hewlett Packard Company, Hewlett Packard Enterprise Business Unit, EG HP Storage, Enterprise...

    Science.gov (United States)

    2013-08-08

    ... DEPARTMENT OF LABOR Employment and Training Administration [TA-W-82,620] Hewlett Packard Company, Hewlett Packard Enterprise Business Unit, EG HP Storage, Enterprise Storage, Servers and Networking... Enterprise Business Unit, EG HP Storage, Enterprise Storage, Servers and Networking Storage Division, APP...

  13. Pulmonary haptoglobin (pHp) is part of the surfactant system in the human lung.

    Science.gov (United States)

    Abdullah, Mahdi; Goldmann, Torsten

    2012-11-20

    Since the existence of pHp was demonstrated, it has been shown that this molecule and its receptor CD163 are regulated by different stimuli. Furthermore, a comparably fast secretion of pHp was described as well as the immuno-stimulatory effects. The intention of this study was to elucidate the role of pHp in the human lungs further. Here we show, by means of confocal microscopy and immune-electron-microscopy, a clear co-localization of pHp with surfactant protein-B in lamellar bodies of alveolar epithelial cells type II. These results are underlined by immunohistochemical stainings in differently fixed human lung tissues, which show pHp in vesicular and released form. The images of the released form resemble the intended position of surfactant in the human alveolus. pHp is secreted by Alveolar epithelial cells type II as previously shown. Moreover, pHp is co-localized with Surfactant protein-B. We conclude that the presented data shows that pHp is a native part of the surfactant system in the human lung. http://www.diagnosticpathology.diagnomx.eu/vs/2563584738239912.

  14. POF and HP1 bind expressed exons, suggesting a balancing mechanism for gene regulation.

    Directory of Open Access Journals (Sweden)

    Anna-Mia Johansson

    2007-11-01

    Full Text Available Two specific chromosome-targeting and gene regulatory systems are present in Drosophila melanogaster. The male X chromosome is targeted by the male-specific lethal complex believed to mediate the 2-fold up-regulation of the X-linked genes, and the highly heterochromatic fourth chromosome is specifically targeted by the Painting of Fourth (POF protein, which, together with heterochromatin protein 1 (HP1, modulates the expression level of genes on the fourth chromosome. Here we use chromatin immunoprecipitation and tiling microarray analysis to map POF and HP1 on the fourth chromosome in S2 cells and salivary glands at high resolution. The enrichment profiles were complemented by transcript profiles to examine the link between binding and transcripts. The results show that POF specifically binds to genes, with a strong preference for exons, and the HP1 binding profile is a mirror image of POF, although HP1 displays an additional "peak" in the promoter regions of bound genes. HP1 binding within genes is much higher than the basal HP1 enrichment on Chromosome 4. Our results suggest a balancing mechanism for the regulation of the fourth chromosome where POF and HP1 competitively bind at increasing levels with increased transcriptional activity. In addition, our results contradict transposable elements as a major nucleation site for HP1 on the fourth chromosome.

  15. Analysis of HP1α regulation in human breast cancer cells

    DEFF Research Database (Denmark)

    Thomsen, Rune; Christensen, Dennis B; Rosborg, Sanne

    2011-01-01

    immunoprecipitation analysis of the HP1α gene showed a decrease in the histone mark for transcriptional activity H3-K36 tri-methylation and RNA polymerase II in invasive breast cancer cells which correlated with a decreased abundance of basal transcription factors at the HP1α promoter. E2F transcription factors...

  16. Relationship between Helicobacter pylori infection (HP) and bone mineral density (BMD) in elderly people.

    Science.gov (United States)

    Fotouk-Kiai, Mahdiye; Hoseini, Seyed Reza; Meftah, Neda; Ghadimi, Reza; Bijani, Ali; Noreddini, Hajighorban; Nematollahi, Hamidreza; Shokri-Shirvani, Javad

    2015-01-01

    Low bone mass is a frequent complication of chronic inflammatory disease. The pathogenesis of osteoporosis in chronic inflammatory disease may be secondary to releases of cytokines such as TNF- and IL6. Chronic gastritis due to helicobacter pylori (HP) infection may lead to decreased bone mineral density (BMD) and predispose patients to osteoporosis. The objective of this study was to determine the BMD status in HP positive patients with gastritis versus HP negative cases. In this cross-sectional study, we enrolled 967 participants aged 60 years old and more from Amirkola Health Study Ageing Project. Seven-hundred and fifty eight HP positive and 209 HP negative patients were analyzed. BMD was measured by dual-energy x-ray absorptiometry (DXA) method in the spine and femoral neck in all participants. The mean age in HP+ and HP- negative patients was 68.3±6.8 and 69.3±7.4 years, respectively. BMD g/cm2 in the spine and femoral neck did not differ between the two groups (P=0.19 and 0.22 respectively). The prevalence of osteoporosis did not also differ across the two groups as well. There was no relationship between the level of antibodies against HP and BMD. According to the findings of this study, H. pylori infection is not associated with BMD changes in the elderly population.

  17. Preliminary assessment of Geant4 HP models and cross section libraries by reactor criticality benchmark calculations

    DEFF Research Database (Denmark)

    Cai, Xiao-Xiao; Llamas-Jansa, Isabel; Mullet, Steven

    2013-01-01

    , U and O in uranium dioxide, Al metal, Be metal, and Fe metal. The native HP cross section library G4NDL does not include data for elements with atomic number larger than 92. Therefore, transuranic elements, which have impacts for a realistic reactor, can not be simulated by the combination of the HP...

  18. The CompHP Core Competencies Framework for Health Promotion in Europe

    Science.gov (United States)

    Barry, Margaret M.; Battel-Kirk, Barbara; Dempsey, Colette

    2012-01-01

    Background: The CompHP Project on Developing Competencies and Professional Standards for Health Promotion in Europe was developed in response to the need for new and changing health promotion competencies to address health challenges. This article presents the process of developing the CompHP Core Competencies Framework for Health Promotion across…

  19. Recurring exon deletions in the haptoglobin (HP) gene associate with lower blood cholesterol levels

    Science.gov (United States)

    Boettger, Linda M.; Salem, Rany M.; Handsaker, Robert E.; Peloso, Gina; Kathiresan, Sekar; Hirschhorn, Joel; McCarroll, Steven A.

    2016-01-01

    Two exons of the human haptoglobin (HP) gene exhibit copy number variation that affects HP multimerization and underlies one of the first protein polymorphisms identified in humans. The evolutionary origins and medical significance of this polymorphism have been uncertain. Here we show that this variation has likely arisen from the recurring reversion of an ancient hominin-specific duplication of these exons. Though this polymorphism has been largely invisible to genome-wide genetic studies to date, we describe a way to analyze it by imputation from SNP haplotypes and find among 22,288 individuals that these HP exonic deletions associate with reduced LDL and total cholesterol levels. We show that these deletions, and a SNP that affects HP expression, are the likely drivers of the strong but complex association of cholesterol levels to SNPs near HP. Recurring exonic deletions in the haptoglobin gene likely enhance human health by lowering cholesterol levels in the blood. PMID:26901066

  20. Does adiponectin level explain the atherogenic properties of Hp 2-2 phenotype in type 2 diabetic patients?

    Science.gov (United States)

    Frand, J; Borislavsky, A; Boaz, M; Matas, Z; Shargorodsky, M

    2013-01-01

    Haptoglobin (Hb) and adiponectin are antioxidant proteins and independent predictors of atherosclerotic vascular disease in diabetic patients. The link between Hp phenotype and circulating adiponectin levels were examined. Diabetic patients were divided into two groups by Hp phenotype: Hp 2-2 group and non-Hp 2-2 group (Hp 2-1 and Hp 1-1). Blood glucose, HbA1C, insulin, lipids, CRP, HOMA-IR, 25OH vitamin D, leptin and adiponectin levels were measured. Pulse wave velocity (PWV) was performed using SphygmoCor (version 7.1, AtCor Medical, Sydney, Australia). PWV was significantly higher in patients homozygous for the 2 allele (Hp 2-2) compared to non-Hp 2-2 patients (Hp 1-1 and Hp 1-2), p Hp 2-2 patients than in non-Hp 2-2 group (p Hp 2-2). These differences were detected despite the lack of by-phenotype differences in glycemic control, blood pressure level or presence of cardiovascular risk factor and suggest an active role of adiponectin in the pathophysiology of vascular disease in this population. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

  1. Cadmium inhibits testis and epididymal acidification in vivo

    Energy Technology Data Exchange (ETDEWEB)

    Caflisch, C.r.p; DuBose, T.D. Jr.

    1986-03-01

    The testis is known to be highly sensitive to functional impairment by cadmium, a widely distributed trace metal. Both vascular compromise and inhibition of Leydig cell androgen production may result in impaired sperm maturation and motility. Recent studies by our laboratory have confirmed the presence of an acid mileau in the testis and epididymis which may play an important role in sperm maturation. In this study the effect of cadmium on luminal acidification was assessed in rat seminiferous tubules, caput and cauda epididymis by glass membrane double-barrelled pH microelectrodes in vivo. Four Sprague-Dawley rats received CdCl/sub 2/ (0.015 mM/kg s.c.) 24 hrs. prior to micropuncture and 4 rats served as controls. Arterial blood gas values were within the normal range and were not different in the two groups. Cadmium resulted in marked alkalinization of seminiferous tubule fluid compared to controls (7.30 +/- 0.01 (15) vs 6.97 +/- 0.01 (25)) (p < 0.001). Similarly, the pH in proximal caput after CdCl/sub 2/ was 7.07 +/- 0.02 (19) a value significantly more alkaline (p < 0.001) than 6.58 +/- 0.02 (24) in untreated animals. In contrast, however, pH in the distal caput was 6.90 +/- 0.03 (19), a value indistinguishable from that observed in controls. In summary, CdCl/sub 2/ administration is associated with marked impairment of acidification in the testis and proximal epididymus while acidification in the distal epididymus remains intact.

  2. Up-regulation of pro-inflammatory factors by HP-PRRSV infection in microglia: implications for HP-PRRSV neuropathogenesis.

    Science.gov (United States)

    Chen, Xin-Xin; Quan, Rong; Guo, Xue-Kun; Gao, Li; Shi, Jishu; Feng, Wen-Hai

    2014-05-14

    Atypical porcine reproductive and respiratory syndrome (PRRS) caused by a highly pathogenic PRRS virus (HP-PRRSV) is characterized by high fever, high morbidity, high mortality, and associated with severe neurological symptoms. Microglia are the resident innate immune cells in central nervous system (CNS), and their activation has been implicated as an important contributor to the pathogenesis of CNS diseases. In the present study, we successfully cultured porcine microglia and demonstrated that microglia could support PRRSV infection and replication in vitro. We further showed that HP-PRRSV infection significantly up-regulated the key inflammatory factors including IL-1β, TNF-α, IL-6, IL-12, IL-8, CXCL10, MCP-1, CCL3, CCL4, and CCL5 in cultured microglia as well as in the CNS of HP-PRRSV-infected pigs. The transcription factors NF-κB and AP-1, which are widely reported to regulate cytokine and chemokine productions, were activated by HP-PRRSV infection in microglia. Meanwhile, we found that HP-PRRSV induced cellular ROS formation in microglia and ROS scavenger was proved to significantly abolish the activation of pro-inflammatory cytokines (IL-1β, TNF-α, IL-6, and IL-8), suggesting that ROS are crucial for pro-inflammatory gene production. Importantly, incubation with supernatants from HP-PRRSV-infected microglia cell culture remarkably induced SH-SY5Y neuroblastoma cell death. Collectively, these results showed that PRRSV infection induced cytokine and ROS up-regulation in microglia, which might contribute to neurotoxicity. These data have implications for us to understand the neuropathogenesis of HP-PRRSV in pigs. Copyright © 2014 Elsevier B.V. All rights reserved.

  3. Testicular sparing surgery in small testis masses: A multinstitutional experience

    Directory of Open Access Journals (Sweden)

    Andrea B. Galosi

    2016-12-01

    Full Text Available Introduction: The incidence of benign testicular tumors is increasing in particular in small lesion incidentally found at scrotal ultrasonography. Primary aim of this study was to perform radical surgery in malignant tumor. Secondary aim was to verify the efficacy of the diagnostic-therapeutic pathway recently adopted in management of small masses with testis sparing surgery in benign lesions. Materials and methods: In this multicenter study, we reviewed all patients with single testis lesion less than 15 mm at ultrasound as main diameter. We applied the diagnostic-therapeutic pathway described by Sbrollini et al. (Arch Ital Urol Androl 2014; 86:397 which comprises: 1 testicular tumor markers, 2 repeated scrotal ultrasound at the tertiary center, 3 surgical exploration with inguinal approach, intraoperative ultrasound, and intraoperative pathological examination. Definitive histology was reviewed by a dedicated uro-pathologist. Results: Twenty-eight patients completed this clinical flowchart. The mean lesion size was 9.3 mm (range 2.5-15. Testicular tumor markers were normal except in a case. Intraoperative ultrasound was necessary in 8/28 cases. We treated 11/28 (39.3% with immediate radical orchiectomy and 17/28 (60.7% with testis-sparing surgery. Definitive pathological results were: malignant tumor in 6 cases (seminoma, benign tumor in 10 cases (5 Leydig tumors, 2 Sertoli tumors, 1 epidermoid cyst, 1 adenomatoid tumor, 1 angiofibroma, benign disease in 11 (8 inflammation with haemorragic infiltration, 2 tubular atrophy, 1 fibrosis, and normal parenchyma in 1 case. We observed a good concordance between frozen section examination and definitive histology. Any malignant tumor was treated conservatively. Any delayed orchiectomy was necessary based on definitive histology. Conclusions: The incidence of benign lesions in 60% of small testis lesions with normal tumor markers makes orchiectomy an overtreatment. Testicular sparing surgery of single

  4. Diagnostic-therapeutic pathway for small lesions of the testis

    Directory of Open Access Journals (Sweden)

    Giulia Sbrollini

    2014-12-01

    Full Text Available Objective of our study was to define a diagnostic-therapeutic pathway for proper treatment of not-palpable testicular masses, that may be benign in 38% of cases. Since the intraoperative diagnosis is difficult to reach in particular in small lesion (< 8 mm and the risk of tissue loss in frozen section analysis occurs frequently, we propose a diagnostic flow chart for the best management of small testis lesions. This proposed protocol has to be shown in details to physicians and patients, who must understand the clinical implications and the risk to undergo a second radical surgery.

  5. FLOOD 3 code conversion from Apollo to HP

    International Nuclear Information System (INIS)

    Lee, Hae Cho

    1996-01-01

    FLOOD3 is a Fortran program used to analyze LOCA Reflood and Post Reflood transients for purpose of mechanistically generating containment sizing mass/energy source terms. The reflood time frame starts when safety injection water first enters the bottom of the core, following the initial LOCA blowdown phase, and the ends when liquid level in the core is high enough to quench the core. The post reflood phase starts at the end of reflood phase and lasts until the end of LOCA transient period. Longterm core cooling is initiated following post reflood phase. FLOOD3 code dose not contain separate capability for longterm cooling analysis. This report firstly describes detailed work carried out for installation of FLOOD3 on Apollo DN10000 and code validation results after installation. Secondly, A series of work is also describes in relation to installation of FLOOD3 on HP 9000/700 series as well as relevant code validation results. Attached is a report on software verification and validation results. 7 refs. (Author) .new

  6. Prevention of H2O2 Induced Oxidative Damages of Rat Testis by Thymus algeriensis.

    Science.gov (United States)

    Guesmi, Fatma; Beghalem, Hamida; Tyagi, Amit K; Ali, Manel Ben; Mouhoub, Ramla Ben; Bellamine, Houda; Landoulsi, Ahmed

    2016-04-01

    We evaluate the effects of Thymus algeriensis (TEO) against hydrogen peroxide (H2O2) toxicity on body and testis weight, testis sperm count, testis lipid peroxidation, and antioxidant enzyme activities in rats. Rats were treated with low (LD) and high dose (HD) of H2O2 (0.1 and 1 mmol/L) in the presence or absence of TEO (150 mg/kg). The results exhibited a significant decrease in body weight and testis weight, in total sperm number decrease (P<0.05), sperm motility and percentage of sperm viability, leading to complete arrest, in sperm flagellar beat frequency by the gavage of 1 mmol/L H2O2 compared to controls. The administration of H2O2 resulted in a significant reduction in testis GSH, GPx, CAT, SOD, and GST activity and significant increase (P<0.05) in MDA concentration compared with the untreated control animals. TEO pre-treatment protected testis from the H2O2 generated oxidative stress. These results were confirmed by histological architecture examinations. H2O2 has the ability to alter the sperm function, characteristics and development of testis. However, TEO is an efficient natural agent, which can prevent the testis from H2O2-induced oxidative damage in rats. Copyright © 2016 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.

  7. Diethylstilbestrol regulates mouse gubernaculum testis cell proliferation via PLC-Ca2+-CREB pathway.

    Science.gov (United States)

    Zhang, Xuan; Ping, Hong-Yan; Li, Jian-Hong; Duan, Shou-Xin; Jiang, Xue-Wu

    2018-01-01

    Recent evidence suggested a positive correlation between environmental estrogens (EEs) and high incidence of abnormalities in male urogenital system, but the mechanism remains unclear. Diethylstilbestrol (DES) is a nonsteroidal synthetic estrogen that disrupts the morphology and proliferation of gubernaculum testis cells, but the underlying mechanism is unclear. In this study, mouse gubernaculum testis cells were pretreated with phospholipase C (PLC) inhibitor U-73122 and then treated with DES. The results demonstrated that U-73122 impaired DES-evoked intracellular Ca2+ mobilization in gubernaculum testis cells and inhibited DES-induced proliferation of gubernaculum testis cells. Mechanistically, we found that U-73122 inhibited DES-induced activation of cAMP-response element binding protein (CREB) in gubernaculum testis cells. In conclusion, these data suggest that the effects of DES on mouse gubernaculum testis cells are mediated by PLC-Ca 2+ -CREB pathway. Environmental estrogens remain a serious threat to male reproductive health, and it is important to understand the mechanism by which EEs affect the male productive system. Here we explore potential mechanisms how the proliferation and contractility of gubernaculum testis cells are regulated by diethylstilbestrol. Our findings provide the first evidence that PLC-Ca 2+ -CREB signalling pathway mediates the nongenomic effects of diethylstilbestrol on gubernaculum testis cells. These findings provide new insight into the role of diethylstilbestrol in the aetiology of male reproductive dysfunction and will help develop better approaches for the prevention and therapy of male reproductive malformation. Copyright © 2017 John Wiley & Sons, Ltd.

  8. Efek ekstrak testis terhadap jumlah implantasi dan jumlah anak pada mencit (Mus musculus

    Directory of Open Access Journals (Sweden)

    Sri Puji Astuti Wahyuningsih

    2012-02-01

    Full Text Available Extract of testis contains the testis specific proteins. The testis proteins may raise immune responses. The immune responses arethe antibodies against testis specific anti-protein. The reaction between the antibodies and the sperms can inhibit fertilization. Thisprocess has contraception effects. This research used 24 female and 20 male mice strain Balb/c. The experiment used the completerandom design with 4 concentration treatments (0, 500, 1000, and 2000 μg extracts of testis and 3 replications. Mice were injected 3times with time interval 21 days. After 15 days from the rising of vaginal plug, 3 mice were dissected and counted the implantation. Theothers mice were waited until delivered of the children. The datas were analyzed by ANOVA and LSD (α = 5%. The results showed thatextract of testis did not influence the amount of implantation at the uterus endometrial, but it decreased the amount of children. Theeffective concentration of extract testis is 2000 μg. So, this study showed that the intraperitoneum injection of the extract of testis hadcontraception effects.

  9. Serous papillary adenocarcinoma of the rete testis: Unusual ultrasonography and pathological findings

    Directory of Open Access Journals (Sweden)

    Mermershtain Wilmosh

    2007-01-01

    Full Text Available We report a case of serous papillary adenocarcinoma of the rete testis in a 22-year-old man. Adenocarcinoma of the rete testis is highly resistant to radiotherapy and any known chemotherapeutic regimen. We recommend radical orchiectomy At last follow up, the patient was well, without any evidence of recurrence, ten years after surgery.

  10. Gene expression profiles in testis of pigs with extreme high and low levels of androstenone

    DEFF Research Database (Denmark)

    Moe, Maren; Meuwissen, Theo; Lien, Sigbjørn

    2007-01-01

    Boar taint is a major obstacle when using uncastrated male pigs for swine production. One of the main compounds causing this taint is androstenone, a pheromone produced in porcine testis. Here we use microarrays to study the expression of thousands of genes simultaneously in testis of high and lo...

  11. Leydig cells in the testis of the rat : heterogeneity, development and similarities with macrophages

    NARCIS (Netherlands)

    R. Molenaar (Rinkje)

    1986-01-01

    textabstractLeydig cells are present in the interstitium of the testis and are responsible for the steroid production of the testis. This production is regulated by the pituitary hormone LH and there are indications that it is also under the influence of local factors. Heterogeneity of cell

  12. utilization of crude testis extract to enhance broiler production in the ...

    African Journals Online (AJOL)

    EKWUEME

    Two experiments were conducted to investigate the possibility of using aqueous testis extracts as growth promoters. In the first experiments, 100 Abore Acre chicks were assigned to four treatments in which the birds were given 0, 10,15 and 20% of a 3.3. % aqueous bull testis extract in drinking water ad libitum from week ...

  13. Concentration of radiolabeled cholesterol in a feminizing adenoma of the testis

    International Nuclear Information System (INIS)

    Leonard, J.M.; Rudd, T.G.; Gurgess, E.C.; Monda, G.A.

    1979-01-01

    Quantitative tissue studies demonstrated increased 19-[ 131 I]-iodocholesterol concentration in a feminizing adenoma of the testis. The potential application of iodocholesterol and its isomers in the detection of steroid-secreting neoplasms of the testis and ovary is suggested

  14. Leiomyoma of Testis –Rare Benign Mimicker of Testicular Malignancy

    Directory of Open Access Journals (Sweden)

    Asif Baliyan

    2017-10-01

    Full Text Available Leiomyomas are benign tumours that originate from any organ containing smooth muscles. The testis is an extremely rare site. We report a case of testicular leiomyoma in a 50-year-old. Testis-associated leiomyomas are a benign and rare disease presenting as a painless, slowly-growing mass. Only histological examination with immunohistochemistry can validate the diagnosis.

  15. Revascularization of the testis using a vascular induction technique: a potential approach for staged orchiopexy in high-undescended testis.

    Science.gov (United States)

    Erçöçen, Ali Riza; Soejima, Kazutaka; Sakurai, Hiroyuki; Yenidünya, Sibel; Kikuchi, Yuji; Nozaki, Motohiro

    2004-02-01

    The present study was designed to determine whether a fasciovascular flap as a vascular carrier could be used to revascularize the undescended testis for avoiding the hazardous effects of the Fowler-Stephens procedure, high division of the spermatic vessels, and for bringing high-undescended testes into the scrotum. A total of 25 Wistar rats were divided into five groups of five rats each. In each group, surgical procedures were performed bilaterally, i.e. ten testes in each group, as follows: sham-operated controls (group 1), undescended testes (group 2), high division of the spermatic vessels (group 3), vascular induction with immediate division of spermatic vessels (group 4), and with delayed division of spermatic vessels (group 5). Evaluations were done by measuring the testicular weight and volume, testicular blood flow, and testicular biopsy scores and by microangiography. A moderate to severe decrease in testicular weight and volume in all experimental groups was observed compared with the sham-operated controls (group 1), but this was significantly less in groups 2 and 5. High division of the spermatic vessels in groups 3 and 4 resulted in a significantly greater decrease in the testicular blood flow, but this did not occur in group 5. Microangiographically, an impaired vascular supply from the deferential artery in group 3 and insufficient revascularization from the fasciovascular carrier in group 4 were observed. However, efficient revascularization stemming from the superficial epigastric artery of the fasciovascular flap was found in group 5. The testicular biopsy scores of groups 2 and 5 were significantly greater than those of groups 3 and 4. The results of the present study demonstrate that the fasciovascular flap as a vascular carrier revascularizes the testis through spermatic vessels after delayed division and provides an adjuvant treatment modality or first-stage procedure in a salvage operation for high-undescended testis during staged

  16. Haplotype association between haptoglobin (Hp2 and Hp promoter SNP (A-61C may explain previous controversy of haptoglobin and malaria protection.

    Directory of Open Access Journals (Sweden)

    Sharon E Cox

    2007-04-01

    Full Text Available Malaria is one of the strongest recent selective pressures on the human genome, as evidenced by the high levels of varying haemoglobinopathies in human populations-despite the increased risk of mortality in the homozygous states. Previously, functional polymorphisms of Hp, coded by the co-dominant alleles Hp1 and Hp2, have been variously associated with several infectious diseases, including malaria susceptibility.Risk of a clinical malarial episode over the course of a malarial transmission season was assessed using active surveillance in a cohort of Gambian children aged 10-72 months. We report for the first time that the major haplotype for the A-61C mutant allele in the promoter of haptoglobin (Hp-an acute phase protein that clears haemoglobin released from haemolysis of red cells-is associated with protection from malarial infection in older children, (children aged >or=36 months, >500 parasites/ul and temperature >37.5 degrees C; OR = 0.42; [95% CI 0.24-0.73] p = 0.002 (lr test for interaction, or=36 months, p = 0.014. Protection was also observed using two other definitions, including temperature >37.5 degrees C, dipstick positive, plus clinical judgement of malaria blinded to dipstick result (all ages, OR = 0.48, [95% CI 0.30-0.78] p = 0.003; >or=36 months, OR = 0.31, [95% CI 0.15-0.62] p = 0.001. A similar level of protection was observed for the known protective genetic variant, sickle cell trait (HbAS.We propose that previous conflicting results between Hp phenotypes/genotypes and malaria susceptibility may be explained by differing prevalence of the A-61C SNP in the populations studied, which we found to be highly associated with the Hp2 allele. We report the -61C allele to be associated with decreased Hp protein levels (independent of Hp phenotype, confirming in vitro studies. Decreased Hp expression may lead to increased oxidant stress and increased red cell turnover, and facilitate the development of acquired immunity, similar to

  17. Sertoli cell tumor arising in a cryptorchid testis presenting as a content of inguinal hernial sac

    Directory of Open Access Journals (Sweden)

    Kusuma Venkatesh

    2016-01-01

    Full Text Available Sertoli cell tumors (SCTs are rare tumors accounting for <1% of all testicular tumors. Here, we report a rare case of SCT in a 60-year-old man presenting as a painless swelling in the right groin since childhood. Clinically, he presented with right-sided inguinal hernia with absence of the right testis. He had normal left testis and had no gynecomastia or infertility. The specimen of hernial sac showed testis with a 1.6 cm × 1.5 cm nodular mass having gray tan-cut surface. Histopathologically, the testis showed atrophy and the nodular portion showed tumor cells arranged in tubular and microcystic pattern, with no solid pattern or necrosis. The diagnosis of SCT was confirmed with immunohistochemical staining for inhibin which showed fine granular cytoplasmic positivity. Cryptorchid testis having SCT and presenting as a content of inguinal hernia is a rare occurrence.

  18. ‘n Vergelykende histomorfologiese assessering van die testis van twee Clarias spesies van die Okavango Delta, Botswana

    Directory of Open Access Journals (Sweden)

    M.L. Mokae

    2012-03-01

    Full Text Available Die testis van twee Clarias species wat in die Okavango Delta voorkom, is histomorfologiesgeassesseer om die verskil in struktuur te beskryf, omdat die twee spesies se testiskleur verskilmet die Clarias ngamensis ‘n swart testis en die Clarias gariepinus ‘n roomkleurige testis.

  19. Test-and-treat strategy for Helicobacter pylori (HP) infection in older patients.

    Science.gov (United States)

    Rotolo, G; Dominguez, L J; Sarakatsianou, V; Mangiaracina, D; Figlioli, F; Barbagallo, M

    2010-01-01

    Despite Helicobacter pylori (HP) infection is highly prevalent in older populations, low rates of HP diagnosis and eradication are reported in older persons, even in geriatric wards. We aim to evaluate the results of a HP-eradicating program in a sample of older patients in relation to dyspeptic symptoms and to endoscopic findings. A pool of 140 subjects (female/ale=86/54, mean age 68.6±5.4 years) with positive C(13)-urea breath test were recruited, clinically evaluated to investigate the presence and characteristics of dyspepsia and abdominal pain, and underwent gastric endoscopic examination. HP-eradication was obtained in 87.9% of subjects with first-line triple therapy, regardless of endoscopic findings. Sixty-eight (48.6%) patients were symptomatic and 72 (51.4%) were asymptomatic. HP-related organic disease in endoscopic examination was frequent (present in 53.6% of the patients) but the distribution of different pathologies were similar in patients with and without symptoms (p=0.86). even if diagnosis and treatment of HP infection in older patients is underestimated, this population should be a priority for HP-eradication since the infection is more frequent, peptic disease and gastric cancer are frequent, and eradication is effective. Copyright © 2009 Elsevier Ireland Ltd. All rights reserved.

  20. Cyclic utilization of HP-β-CD in the bioconversion of cortisone acetate by Arthrobacter simplex.

    Science.gov (United States)

    Shen, Yanbing; Wang, Fang; Wang, Yanping; Zhao, Qing; Wang, Min

    2016-04-01

    To establish a method for the recovery and reutilization of hydroxypropyl-β-cyclodextrin (HP-β-CD) to lower the cost of its industrial application in cortisone acetate bioconversion. HP-β-CD is not degraded by Arthrobacter simplex CPCC140451 (ASP) resting cells and 96.4 % HP-β-CD could be recovered by isobutyl acetate extraction. Moreover, the inclusion ability of recovered HP-β-CD barely decreased. The saccharide metabolic and catalytic activities of ASP were greater in the aqueous phase after extracting with isobutyl acetate than other organic solvents. Cyclic utilization tests showed that cortisone acetate conversion ratio was 91.0 % after eight cycles and reached 95.7 % with 0.2-0.6 mM HP-β-CD. Furthermore, >90 % conversion ratio was reached per cycle through a co-cyclic-utilization method with HP-β-CD and immobilized ASP. Cortisone acetate conversion ratio in the HP-β-CD cyclic-utilization method is promising for industrial applications. The method can also be expanded to other CDs and other hydrophobic compounds bioconversion.

  1. Distribution of the DAZ gene transcripts in human testis.

    Directory of Open Access Journals (Sweden)

    J B Warchoł

    2004-07-01

    Full Text Available Involvement of variety of genes, especially located on Y chromosome, is critical for the regulation of spermatogenesis. In particular, fertility candidate genes such as deleted in azoospermia (DAZ are believed to have important function in sperm production, since DAZ is frequently deleted in azoospermic and severy oligozoospermic men. The role of the DAZ gene is supported by its exclusive expression in the testis and by its deletion in about 10% of azoospermic and severely oligozoospermic patients. The distribution of DAZ transcripts in seminiferous epithelium of human testis is reported in the present study. The use of Adobe Photoshop and Scion Image softwares allowed for semi-quantitative analysis of in situ RT-PCR (ISRT-PCR results. The intensity of ISRT-PCR product's fluorescence was different within individual seminiferous tubules. It was clearly shown by using the pseudocolour scale and transforming the intensity of the fluorescence into levels of greyscale images. The more intense fluorescence characterised single spermatogonia and those organized in small groups inside separate tubules. The most intense accumulation of DAZ mRNA was observed in spermatogonia.

  2. Stage I seminoma of the testis. Adjuvant radiotherapy or surveillance?

    International Nuclear Information System (INIS)

    Allhoff, E.P.; Liedke, S.; De Riese, W.; Stief, C.; Schneider, B.

    1991-01-01

    Lately the role of radiotherapy in stage I seminoma of the testis has been questioned by some authors who reported on a ''surveillance'' strategy for these patients. Since 1980, 124 patients with seminoma of the testis have been referred to this institution; 97 of 116 patients analysed presented with stage I disease and 10 of these had elevated levels of βHCG. A total of 64 patients were given radiotherapy after orchiectomy and 33 entered a surveillance protocol. After a median follow-up of 48 months, 3 patients in the surveillance group relapsed after 5, 13 and 49 months and 2 of the irradiated patients did so after 25 and 33 months. Elevation of βHCG was not significant because none of these patients showed progression. A low rate of progression and excellent survival are associated with standard treatment (orchiectomy and radiotherapy) and good results have been achieved with chemotherapy in cases of relapse. A surveillance policy is not recommended in stage I seminoma because of its slower growth compared with non-seminomatous germ cell tumours (NSGTC), the absence of a specific tumour marker, the 10% risk of occult metastases and the 3-fold higher progression rate compared with irradiated patients. We suggest the use of a reduced dosage and radiation field. (author)

  3. Effect of hydrocele on appendix testis in children.

    Science.gov (United States)

    Józsa, Tamás; Telek, Andrea; Kutasy, Balázs; Benyó, Mátyás; Csanádi, Gábor; Kovács, Ilona; Balla, György; Flaskó, Tibor; Csernoch, László; Kiss, Csongor

    2009-11-01

    The purpose of this study was to investigate the effects of an elevated hydrostatic pressure of hydrocele on the structural integrity and steroid receptor expression pattern of the appendix testis in children. Twenty-six testicular appendages were obtained from boys (aged between 13 and 79 months, mean 40 months) who underwent surgical exploration because of hydrocele or congenital inguinal hernia. The tissue sections of testicular appendages were stained with hematoxylin-eosin. Immunohistochemistry and immunofluorescence laser microscopy were performed using monoclonal mouse anti-human receptors against androgen and estrogen receptors. Patients were divided into three groups: group A (n = 8) represented patients with groin hernia without hydrocele, who served as control group; group B (n = 7) represented patients with communicating hydrocele; and group C (n = 11) represented patients with noncommunicating hydrocele. The tissue sections of appendix testis expressed both androgen and estrogen receptors in all patients in groups A and B, and epithelial destruction was not present. The presence of androgen receptor (two of 11, P hydrocele did not influence the receptor expression pattern and the anatomic structure of testicular appendages, whereas noncommunicating hydrocele caused damage as indicated by the absence of steroid receptors and destruction of the epithelial surface. A better understanding of the physiological role of testicular appendages may change the indications of surgical treatment in patients with noncommunicating hydrocele.

  4. Flow cytometry of DNA in mouse sperm and testis nuclei

    Energy Technology Data Exchange (ETDEWEB)

    Meistrich, M.L. (Univ. of Texas, Houston); Lake, S.; Steinmetz, L.L.; Gledhill, B.L.

    1978-01-01

    Mutations that occur in spermatogenic cells may be expressed as changes in DNA content, but developmentally-dependent alteration of its staining properties complicates the quantitation of DNA in individual germ cells. These alterations have been studied with flow cytometric techniques. Nuclei from mouse testis cells and sperm were stained by the acriflavine--Feulgen method. The fluorescence intensity frequency distribution of nuclei of testis cells was characterized by 2 major and 5 minor peaks. Nuclei sorted from the various peaks with a fluorescence-activated cell sorter were identified microscopically. These data were confirmed by generation of peaks with nuclei prepared from cell suspensions enriched in specific cell types. One of the major peaks corresponded to round spermatid nuclei. The other major peak, located at 0.6 of the fluorescence intensity of the round nuclei, corresponded to elongated spermatid nuclei. Purified nuclei of epididymal and vas deferens spermatozoa displayed asymmetric fluorescence distributions. A minor peak at 0.8 the intensity of the round spermatid nuclei was tentatively assigned to elongating spermatids. 2 of the minor peaks, located at 1.7 and 2.0 times the fluorescence intensity of the round nuclei, corresponded to clumps of 2 haploid and diploid nuclei.

  5. Figure 1. Prediction of ScHP1 transmembrane domains I to XIV. (http ...

    Indian Academy of Sciences (India)

    Figure 2. Comparison of the ScHP1 amino acid sequence with H. +. -PPase from other species. The conserved domains GGG,. DVGADLVGKVE, DNVGDNVGD, EYYT and GNTTAA were found in the sequence alignment (shown in red boxes).

  6. Figure 1. Prediction of ScHP1 transmembrane domains I to XIV. (http ...

    Indian Academy of Sciences (India)

    embnet.org/software/TMPRED_form.html). Page 2. Figure 2. Comparison of the ScHP1 amino acid sequence with H. +. -PPase from other species. The conserved domains GGG,. DVGADLVGKVE, DNVGDNVGD, EYYT and GNTTAA were found ...

  7. hp-HGS strategy for inverse 3D DC resistivity logging measurement simulations

    KAUST Repository

    Gajda-Zaǵorska, Ewa

    2012-06-02

    In this paper we present a twin adaptive strategy hp-HGS for solving inverse problems related to 3D DC borehole resistivity measurement simulations. The term “simulation of measurements” is widely used by the geophysical community. A quantity of interest, voltage, is measured at a receiver electrode located in the logging instrument. We use the self-adaptive goal-oriented hp-Finite Element Method (hp-FEM) computer simulations of the process of measurements in deviated wells (when the angle between the borehole and formation layers are < 90 deg). We also employ the hierarchical genetic search (HGS) algorithm to solve the inverse problem. Each individual in the population represents a single configuration of the formation layers. The evaluation of the individual is performed by solving the direct problem by means of the hp-FEM algorithm and by comparison with measured logging curve. We conclude the paper with some discussion on the parallelization of the algorithm.

  8. [Comparison of port needle with safety device between Huber Plus (HP) and Poly PERF Safe (PPS)].

    Science.gov (United States)

    Shimono, Chigusa; Tanaka, Atsuko; Fujita, Ai; Ishimoto, Miki; Oura, Shoji; Yamaue, Hiroki; Sato, Morio

    2010-05-01

    An embedded port is frequently used for outpatients with advanced cancer in central venous chemotherapy or hepatic arterial chemoinfusion. The port needle with a safety device in an ambulatory treatment center is indispensable for medical employees and patient plus family to reduce the risk of a needle puncture accident and to prevent iatrogenic infection. The port needle with safety system has been already introduced in our chemotherapy center. There are two types of port needle with safety device; Huber Plus (HP, Medicon Co., Ltd.) and POLY PERF Safe (PPS, Pyolax Device, Co., Ltd.). The comparison of the feasibility between HP and PPS was conducted by both medical employees and patients plus family using an inquiry score method. HP was highly regarded for its stability plus fixation and PPS for its usefulness in puncture and extraction of the needle. PPS was found to be preferable to HP based on the overall evaluation.

  9. Investigating tumor perfusion and metabolism using multiple hyperpolarized 13C compounds: HP001, pyruvate and urea

    DEFF Research Database (Denmark)

    von Morze, Cornelius; Larson, Peder E.Z.; Hu, Simon

    2012-01-01

    by spectroscopic imaging of hyperpolarized [1-13C]pyruvate would be of great value in exploring the relationship between perfusion and metabolism in cancer. In preclinical normal murine and cancer model studies, we performed both dynamic multislice imaging of the specialized hyperpolarized perfusion compound HP001......The metabolically inactive hyperpolarized agents HP001 (bis-1,1-(hydroxymethyl)-[1-13C]cyclopropane-d8) and urea enable a new type of perfusion magnetic resonance imaging based on a direct signal source that is background-free. The addition of perfusion information to metabolic information obtained...... of separate dynamic HP001 imaging and copolarized pyruvate/urea imaging were compared. A strong and significant correlation (R=0.73, P=.02) detected between the urea and HP001 data confirmed the value of copolarizing urea with pyruvate for simultaneous assessment of perfusion and metabolism....

  10. A novel insight into the oxidoreductase activity of Helicobacter pylori HP0231 protein.

    Directory of Open Access Journals (Sweden)

    Paula Roszczenko

    Full Text Available BACKGROUND: The formation of a disulfide bond between two cysteine residues stabilizes protein structure. Although we now have a good understanding of the Escherichia coli disulfide formation system, the machineries at work in other bacteria, including pathogens, are poorly characterized. Thus, the objective of this work was to improve our understanding of the disulfide formation machinery of Helicobacter pylori, a leading cause of ulcers and a risk factor for stomach cancer worldwide. METHODS AND RESULTS: The protein HP0231 from H. pylori, a structural counterpart of E. coli DsbG, is the focus of this research. Its function was clarified by using a combination of biochemical, microbiological and genetic approaches. In particular, we determined the biochemical properties of HP0231 as well as its redox state in H. pylori cells. CONCLUSION: Altogether our results show that HP0231 is an oxidoreductase that catalyzes disulfide bond formation in the periplasm. We propose to call it HpDsbA.

  11. Anatomy and histology of the scrotal ligament in adults: inconsistency and variability of the gubernaculum testis.

    Science.gov (United States)

    Cavalie, G; Bellier, Alexandre; Marnas, G; Boisson, B; Robert, Y; Rabattu, P Y; Chaffanjon, P

    2018-04-01

    The anatomy of gubernaculum testis (GT) is often discussed; however, the postnatal anatomy of the GT or scrotal ligament (SL) is rarely described. Hence, we performed an anatomical and histological study to analyze histologically the structures between testis and scrotum. We performed anatomical dissections on 25 human fresh cadavers' testes. Each testis was removed with its envelopes and macroscopically analyzed. Then samples were included for histological study. Finally, they were analyzed under microscope, looking for attachments between testis, epididymis and scrotal envelopes. The absence of proximal and distal attachment was found in 56.0% of cases. Looking at the proximal attachment of the SL, the main one found is the epididymal attachment (28.0%), whereas no cases of testis attachment was found. Distally, there are more variations with scrotal attachment (12%) and cremaster attachment (12.0%). We found a significant prevalence of multiple adherences in 16.0% of cases too. Finally, in 15 cases (57.7%) an attachment is present between testis and epididymis, as it is commonly described. In the majority of cases there is no attachment of the lower pole of the testis and epididymis and these structures remain free. So it seems that the SL disappears with aging. Moreover, there is not only one kind of ligamentous attachment, but a high variability of attachments at the lower pole of the testiculo-epididymal structure. When it exists, this structure is never a real ligament and it seems more appropriate to use the term "attachments".

  12. Helicobacter pylori HP0231 Influences Bacterial Virulence and Is Essential for Gastric Colonization

    OpenAIRE

    Zhong, Yu; Anderl, Florian; Kruse, Tobias; Schindele, Franziska; Jagusztyn-Krynicka, Elzbieta Katarzyna; Fischer, Wolfgang; Gerhard, Markus; Mejias-Luque, Raquel

    2016-01-01

    The Dsb protein family is responsible for introducing disulfide bonds into nascent proteins in prokaryotes, stabilizing the structure of many proteins. Helicobacter pylori HP0231 is a Dsb-like protein, shown to catalyze disulfide bond formation and to participate in redox homeostasis. Notably, many H. pylori virulence factors are stabilized by the formation of disulfide bonds. By employing H. pylori HP0231 deficient strains we analyzed the effect of lack of this bacterial protein on the funct...

  13. [Preparation of naringenin by enzymolysis of naringin-HP-beta-CD].

    Science.gov (United States)

    Cui, Li; Zhang, Zhenhai; Sun, E; Jia, Xiaobin; Qian, Qian; Jiang, Yanrong

    2012-02-01

    To determine the major factors affecting the conversion efficiency of naringin-HP-beta-CD that was enzymed to prepare naringenin were determined and select the process condition with high conversion efficiency, stable and suitable for industrial production. The dropping method was used to prepare naringin-HP-beta-CD, which was hydrolyzed by snailase to obtain naringenin. With the bioconversion rate as the index, the effects of pH value, temperature, reaction time, dosage of enzyme and concentration of naringin-HP-beta-CD on conversion rate of naringenin were detected for the purpose of optimizing the preparation condition. the conversion efficiency of naringin-HP-beta-CD was verified by scanning calorimetry, and the Hydrolysis product was identified by H-NMR, and 13C-NMR. The optimum enzymolysis of naringin-HP-beta-CD with snailase was 98.4% under the conditions of 37 degrees C, a pH 5.0 acetic acid- sodium acetate buffer solution for 12 hours. The substrate concentration was 30 g x L(-1) and the weight ratio of enzyme and substrate was 3: 5. Under the optimum enzymolysis condition, the conversion rate of naringin-HP-beta-CD was higher than naringin that was not entrapped with HP-beta-CD, with 272.25 reaction product relative molecules. The structure of naringenin was confirmed by the analysis of 1H-NMR and 13C-NMR. Naringin which is entrapped with HP-beta-CD to prepare naringenin can significantly improve the conversion efficiency by shortening the reaction time, increasing the concentration of the substrate and reducing the amount of enzyme. Therefore, the process is stable and it was suitable for industrialization.

  14. The HP0256 gene product is involved in motility and cell envelope architecture of Helicobacter pylori

    LENUS (Irish Health Repository)

    Douillard, Francois P

    2010-04-08

    Abstract Background Helicobacter pylori is the causative agent for gastritis, and peptic and duodenal ulcers. The bacterium displays 5-6 polar sheathed flagella that are essential for colonisation and persistence in the gastric mucosa. The biochemistry and genetics of flagellar biogenesis in H. pylori has not been fully elucidated. Bioinformatics analysis suggested that the gene HP0256, annotated as hypothetical, was a FliJ homologue. In Salmonella, FliJ is a chaperone escort protein for FlgN and FliT, two proteins that themselves display chaperone activity for components of the hook, the rod and the filament. Results Ablation of the HP0256 gene in H. pylori significantly reduced motility. However, flagellin and hook protein synthesis was not affected in the HP0256 mutant. Transmission electron transmission microscopy revealed that the HP0256 mutant cells displayed a normal flagellum configuration, suggesting that HP0256 was not essential for assembly and polar localisation of the flagella in the cell. Interestingly, whole genome microarrays of an HP0256 mutant revealed transcriptional changes in a number of genes associated with the flagellar regulon and the cell envelope, such as outer membrane proteins and adhesins. Consistent with the array data, lack of the HP0256 gene significantly reduced adhesion and the inflammatory response in host cells. Conclusions We conclude that HP0256 is not a functional counterpart of FliJ in H. pylori. However, it is required for full motility and it is involved, possibly indirectly, in expression of outer membrane proteins and adhesins involved in pathogenesis and adhesion.

  15. [Isolation of a wild-type virulent phage of Helicobacter pylori and its simulated treatments of gastrointestinal Hp in vitro].

    Science.gov (United States)

    Wan, Xue-qin; Tang, Dong-sheng; Liu, Ai-ping; Tan, Shu-yi; Li, Wan-kelan; Kuang, Jia; Li, Hong-ming

    2011-02-01

    To isolate the wild-type virulent phage of Helicobacter pylori (Hp) and simulate the treatments in vitro to investigate the methods for oral Hp-assisted penetration of the phage through the gastric barrier and offspring phage release for infection and treatment of gastrointestinal Hp. The Hp strain was cultured with the candle cylinder method and the virulent phage was isolated by single plate or double plate experiment. A simulated gastric juice was applied and the bactericidal effect of the phage was tested with double flats experiment. After a 1.5-h treatment in simulated gastric juice, the orally derived Hp-borne phage was still capable of forming plaques while the control phage was not. The oral Hp can help the phage resist the gastric juice and then infect the gastrointestinal Hp.

  16. Informative gene selection using Adaptive Analytic Hierarchy Process (A2HP

    Directory of Open Access Journals (Sweden)

    Abhishek Bhola

    2017-12-01

    Full Text Available Gene expression dataset derived from microarray experiments are marked by large number of genes, which contains the gene expression values at different sample conditions/time-points. Selection of informative genes from these large datasets is an issue of major concern for various researchers and biologists. In this study, we propose a gene selection and dimensionality reduction method called Adaptive Analytic Hierarchy Process (A2HP. Traditional analytic hierarchy process is a multiple-criteria based decision analysis method whose result depends upon the expert knowledge or decision makers. It is mainly used to solve the decision problems in different fields. On the other hand, A2HP is a fused method that combines the outcomes of five individual gene selection ranking methods t-test, chi-square variance test, z-test, wilcoxon test and signal-to-noise ratio (SNR. At first, the preprocessing of gene expression dataset is done and then the reduced number of genes obtained, will be fed as input for A2HP. A2HP utilizes both quantitative and qualitative factors to select the informative genes. Results demonstrate that A2HP selects efficient number of genes as compared to the individual gene selection methods. The percentage of deduction in number of genes and time complexity are taken as the performance measure for the proposed method. And it is shown that A2HP outperforms individual gene selection methods.

  17. Aspiration biopsy of testis: another method for histologic examination

    International Nuclear Information System (INIS)

    Nseyo, U.O.; Englander, L.S.; Huben, R.P.; Pontes, J.E.

    1984-01-01

    The most important method for evaluating the pathogenesis of male infertility is open testicular biopsy. Herein the authors describe a method of aspiration biopsy of testis for histologic examination. Sexually mature dogs and rats treated with chemotherapeutic agents and ionizing radiation were followed with periodic testicular aspiration biopsy during and after treatment. The histologic findings from the aspiration biopsy compare with the results of routine histologic examination in assessing spermatogenetic activity and delineating pathologic changes. The puncture in the experimental animals was performed under general anesthesia. In human patients testicular biopsy could be done under local anesthesia in an outpatient clinic. The procedure would be less painful, minimally invasive, and more cost-effective

  18. Testis cancer. Ichthyosis constitutes a significant risk factor

    DEFF Research Database (Denmark)

    Lykkesfeldt, G; Bennett, P; Lykkesfeldt, A E

    1991-01-01

    Testis cancer and ichthyosis are both relatively rare diseases. Hence the finding of six individuals with both these conditions in a small population with testicular cancer is highly conspicuous and indicates some kind of connection among such persons. Despite the identical clinical appearances...... of their ichthyoses, three of the ichthyotic subjects had no measurable activity of the enzyme, steroid sulfatase (STS) in leucocytes, a distinct characteristic of recessive X-linked ichthyosis (RXLI). However, the remaining three subjects had normal STS activity, a strong indicator of autosomal dominant ichthyosis...... (ADI). The STS activity in patients with testicular cancer who do not have ichthyosis (N = 30) was also within the normal range. The patients with testicular cancer with no skin disease had elevated serum levels of 4-androstenedione (4-AD), follicle stimulating hormone (FSH), and luteinizing hormone...

  19. [Metastasis of germ cell tumors of the testis].

    Science.gov (United States)

    Machover, D; Goldschmidt, E

    1991-01-01

    The development of metastases from germ cell tumours of the testis is studied in terms of its histopathological, ontogenetic, anatomical and evolutive aspects. The treatment of non-seminomatous germ cell tumours and pure seminomas is analysed separately. The prognostic factors defined by the risk of failure of treatment are described and the medical and surgical strategies or combinations of both modalities are proposed for each stage of these cancers. The major points and novelties include: the usual histological polymorphism of these tumours. The markers include alpha-foetoprotein and HCG. Combination chemotherapy, essentially the EBP sequence, is the treatment for non-seminomatous germ cell tumours. In the case of failure, toxic sequences can be used followed by autologous bone marrow transplantation. In the case of persistent lesions (lung, mediastinum, liver, retroperitoneum), salvage surgery may be useful. Metastatic seminomas are treated by radiotherapy and/or chemotherapy, depending on their stage.

  20. Ultrastructure of spermatogenesis in the testis of Paragonimus heterotremus.

    Science.gov (United States)

    Uabundit, Nongnut; Kanla, Pipatphong; Puthiwat, Phongphithak; Arunyanart, Channarong; Chaiciwamongkol, Kowit; Maleewong, Wanchai; Intapan, Pewpan M; Iamsaard, Sitthichai; Hipkaeo, Wiphawi

    2013-12-01

    Lung fluke, Paragonimus heterotremus, is a flatworm causing pulmonary paragonimiasis in cats, dogs, and humans in Southeast Asia. We examined the ultrastructure of the testis of adult P. heterotremus with special attention to spermatogenesis and spermiogenesis using scanning and transmission electron microscopy. The full sequence of spermatogenesis and spermiogenesis, from the capsular basal lamina to the luminal surface, was demonstrated. The sequence comprises spermatogonia, spermatocytes with obvious nuclear synaptonemal complexes, spermatids, and eventual spermatozoa. Moreover, full steps of spermatid differentiation were shown which consisted of 1) early stage, 2) differentiation stage representing the flagella, intercentriolar body, basal body, striated rootlets, and electron dense nucleus of thread-like lamellar configuration, and 3) growing spermatid flagella. Detailed ultrastructure of 2 different types of spermatozoa was also shown in this study.

  1. Local Actions of Melatonin in Somatic Cells of the Testis.

    Science.gov (United States)

    Frungieri, Mónica Beatriz; Calandra, Ricardo Saúl; Rossi, Soledad Paola

    2017-05-31

    The pineal hormone melatonin regulates testicular function through the hypothalamic-adenohypophyseal axis. In addition, direct actions of melatonin in somatic cells of the testis have been described. Melatonin acts as a local modulator of the endocrine activity in Leydig cells. In Sertoli cells, melatonin influences cellular growth, proliferation, energy metabolism and the oxidation state, and consequently may regulate spermatogenesis. These data pinpoint melatonin as a key player in the regulation of testicular physiology (i.e., steroidogenesis, spermatogenesis) mostly in seasonal breeders. In patients with idiopathic infertility, melatonin exerts anti-proliferative and anti-inflammatory effects on testicular macrophages, and provides protective effects against oxidative stress in testicular mast cells. Consequently, melatonin is also involved in the modulation of inflammatory and oxidant/anti-oxidant states in testicular pathology. Overall, the literature data indicate that melatonin has important effects on testicular function and male reproduction.

  2. Recurrence of acute myeloid leukemia in cryptorchid testis: case report

    Energy Technology Data Exchange (ETDEWEB)

    Góes, Luccas Santos Patto de [Hospital do Servidor Público Municipal de São Paulo, São Paulo, SP (Brazil); Lopes, Roberto Iglesias [Hospital do Servidor Público Municipal de São Paulo, São Paulo, SP (Brazil); Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP (Brazil); Campos, Octavio Henrique Arcos [Hospital do Servidor Público Municipal de São Paulo, São Paulo, SP (Brazil); Oliveira, Luiz Carlos Neves de; Sant' Anna, Alexandre Crippa; Dall' Oglio, Marcos Francisco; Srougi, Miguel [Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP (Brazil)

    2014-07-01

    A 23-year-old male with a history of bone marrow transplant for acute myeloid leukemia. He presented a large mass in the right inguinal region 5 years ago. Upon physical examination, right-sided cryptorchidism was observed. The tumor markers alpha-fetoprotein and beta-HCG were within normalcy range and lactate dehydrogenase was raised. Computed tomography of the abdomen and pelvis revealed right testicular mass in contiguity with the inguinal canal to the ipsilateral retroperitoneum, associated with right hydronephrosis. Due to the risk of germ-cell tumor in undescended testicle, the patient underwent radical right orchiectomy. The pathological examination showed recurrence of acute myeloid leukemia in the testis. He was referred to oncology for adjuvant therapy. Our literature review found no similar cases described.

  3. Live Imaging of the Drosophila Testis Stem Cell Niche.

    Science.gov (United States)

    Greenspan, Leah J; Matunis, Erika L

    2017-01-01

    Live imaging of adult tissue stem cell niches provides key insights into the dynamic behavior of stem cells, their differentiating progeny, and their neighboring support cells, but few niches are amenable to this approach. Here we discuss a technique for long-term live imaging of the Drosophila testis stem cell niche. Culturing whole testes ex vivo for up to 12.5 h allows for tracking of cell-type specific behaviors under normal and various chemically or genetically modified conditions. Fixing and staining tissues after live imaging allows for the molecular confirmation of cell identity and behavior. Utilization of live imaging in intact niches will facilitate further understanding of the cellular and molecular mechanisms that regulate stem cell function in vivo.

  4. Screening for carcinoma in situ of the contralateral testis in patients with germinal testicular cancer

    DEFF Research Database (Denmark)

    Berthelsen, J G; Skakkebaek, N E; von der Maase, H

    1982-01-01

    Two hundred and fifty biopsy specimens from the contralateral testis in patients with unilateral germinal testicular cancer were analysed by light microscopy for carcinoma-in-situ changes. Changes were found in 13 (5.2%) patients. One-third of patients with an atrophic contralateral testis (volume...... of cryptorchidism or both had been screened. Since the natural course of carcinoma in situ in the contralateral testis of patients with germinal testicular cancer has not been established, the patients are being re-evaluated frequently. To date two patients with carcinoma in situ have developed a second cancer....

  5. Examination for intratubular germ cell neoplasia at operation for undescended testis in boys

    DEFF Research Database (Denmark)

    Cortes, Dina; Thorup, Jørgen Mogens; Frisch, M

    1994-01-01

    1 nonseminoma of the contralateral testis, which had been treated before surgery for an undescended testis, 1 nonseminoma found at followup, 1 seminoma and 2 intratubular germ cell neoplasms. Of the later 3 patients 1 had a 45,X/46,XY karyotype and 2 had abnormal external genitalia. Previous...... testicular biopsy from the patient in whom nonseminoma was noted at followup showed Sertoli cells only. We recommend that testicular biopsy be performed at operation for undescended testis in boys with abnormal sex chromosomes, particularly 45,X/46,XY karyotype, and in those with abnormal external genitalia...

  6. Primary adenocarcinoma of the rete testis: A case report and review of the literature.

    Science.gov (United States)

    Tian, Ye; Yao, Wenqing; Yang, Lulu; Wang, Jianzhong; Wazir, Romel; Wang, Kunjie

    2014-02-01

    Primary adenocarcinoma of the rete testis is an extremely rare extratesticular neoplasm. Due to its low occurrence and the scarcity of data, sonographic characteristics of adenocarcinoma of the rete testis are still poorly defined. A 46-year-old male complained of swelling and pain in the right side of the scrotum. No associated symptoms were observed. Anti-tuberculosis chemotherapy yielded no response. Postoperative pathology revealed a diagnosis of poorly-differentiated adenocarcinoma of the rete testis. Using the ultrasonography features observed, combined with a review of current literature, the diagnosis and differential diagnosis of this neoplasm are presented.

  7. Estrogen receptor-mediated transcriptional activity of genistein in the mouse testis.

    Science.gov (United States)

    Montani, C; Penza, M; Jeremic, M; Rando, G; Ciana, P; Maggi, A; La Sala, G; De Felici, M; Di Lorenzo, D

    2009-04-01

    Here we show that genistein, through an estrogen receptor-mediated action, modulates gene expression in the mouse testis throughout development. Genistein passed from the lactating mother to the suckling offspring at levels sufficient to activate gene expression in the testis of the pups. Testis are already responsive to genistein as well as to estradiol at day 14.5 of fetal development. Activation of luciferase correlates with an activation of cell proliferation. In conclusion, our results show that genistein affects reproductive organs of male mice at all developmental ages.

  8. Expression of the GM2 activator protein in mouse testis.

    Science.gov (United States)

    Li, Yu-Teh; Li, Su-Chen; Chen, I-Li

    2017-12-01

    The GM2-activator protein (GM2-AP), revealed by Li et al. in 1973 in human liver, was initially identified as a protein cofactor that stimulated β-hexosaminidase A to hydrolyze N-acetylgalactosamine from GM2 ganglioside. This cofactor was found to be missing in human variant AB Tay-Sachs disease. Over the years, the GM2-AP has also been shown to be involved in kidney vesicular transport, lipid presentation by CD1 molecule to T-cells, and interaction of human sperm with zona pellucida. Since the expression of the GM2-AP via mRNA detection in mouse tissues was found to be the highest in testis, we became interested in the localization of the GM2-AP at cellular level in mouse testis during spermatogenesis. Using immunohistochemical analysis and electron microscopy, we found that the GM2-AP was predominantly localized in the basal cytoplasm and the attenuated processes of Sertoli cells. The stained structure appeared to be lysosomes. The most interesting finding was the association of the GM2-AP with the acrosomal apparatus in early spermatids. A modest to intense staining was observed in some acrosomal granules and acrosomal caps. The GM2-AP seemed to disappear from acrosomal caps in the later stage of spermatids, in which the nucleus became elongated and condensed. These results suggest that the GM2-AP may be involved in the normal functions of Sertoli cells and play important roles during the development of acrosomal caps in the early spermatids. Copyright © 2017 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.

  9. 75 FR 62424 - EDS, an HP Company (Re-Branded as HP-Enterprise Services) Including On-Site Workers From: Abel...

    Science.gov (United States)

    2010-10-08

    ... Floyd and Company, Trinity Government SYS a Private Co, Verizon Network Integration Corp, Vision... technology (IT) services. New information shows that worker separations have occurred involving virtual employees across the United States under the control of the Plano, Texas location of EDS, an HP Company (Re...

  10. Expression of immunoglobulin gene with classical V-(D)-J rearrangement in mouse testis and epididymis.

    Science.gov (United States)

    Huang, Jing; Zhang, Li; Ma, Teng; Zhang, Pei; Qiu, Xiaoyan

    2009-04-01

    Immunoglobulin (Ig) molecules have thus far been found only to be produced by differentiated B lymphocytes. As we know, immune privilege in the normal adult mouse testis exists to make these privileged sites generally free of patrolling immune cells and Igs. However, in this study, the expression of Ig in normal adult mouse testis and epididymis was detected. First, by IHC, a strong pattern of Ig expression was detected in the spermatogenic cells of adult mouse testis at different development stages. Second, by Western blot analysis, different strong bands of Igs in mouse testicular spermatogenic cells and epididymal epithelial cell extract were recognized using specific antibodies against IgG. More importantly, by ISH and cell sorting-related RT-PCR, rearranged Ig gamma chain and kappa chain transcripts were expressed in testicular spermatogenic cells and epididymal epithelial cells. These results suggested that Ig in testis and epididymis was mainly produced by adult mouse testicular spermatogenic cells and epididymal epithelial cells.

  11. Expression and Location of Glucose-regulated Protein 78 in Testis and Epididymis

    Directory of Open Access Journals (Sweden)

    W Wang

    2014-04-01

    Full Text Available Objective: To know the role of glucose-regulated protein 78 (GRP78/BiP/HSPA5 in spermatogenesis and its expression and location in the testis and epididymis. Methods: Immunohistochemistry and Western blot were used to detect GRP78 location and expression in the testis and epididymis. Results: Glucose-regulated protein 78 was observed in spermatocytes, round spermatids and interstitial cells of the testis and in principal cells of the epididymis. Glucose-regulated protein 78 was first detected in the rat testis at postnatal day 14. Thereafter, the protein level increased gradually with age and was maintained at a high and stable state after postnatal day 28. In the rat, GRP78 was expressed in the principal cells but not in clear cells of the epididymis. Conclusion: Glucose-regulated protein 78 participates in the process of spermatogenesis.

  12. Myotube migration to cover and shape the testis of Drosophila depends on Heartless, Cadherin/Catenin, and myosin II

    Directory of Open Access Journals (Sweden)

    Silke Rothenbusch-Fender

    2017-12-01

    Full Text Available During Drosophila metamorphosis, nascent testis myotubes migrate from the prospective seminal vesicle of the genital disc onto pupal testes and then further to cover the testes with multinucleated smooth-like muscles. Here we show that DWnt2 is likely required for determination of testis-relevant myoblasts on the genital disc. Knock down of fibroblast growth factor receptor (FGFR heartless by RNAi and a dominant-negative version revealed multiple functions of Heartless, namely regulation of the amount of myoblasts on the genital disc, connection of seminal vesicles and testes, and migration of muscles along the testes. Live imaging indicated that the downstream effector Stumps is required for migration of testis myotubes on the testis towards the apical tip. After myoblast fusion, myosin II is needed for migration of nascent testis myotubes, in which Thisbe-dependent fibroblast growth factor (FGF signaling is activated. Cadherin-N is essential for connecting these single myofibers and for creating a firm testis muscle sheath that shapes and stabilizes the testis tubule. Based on these results, we propose a model for the migration of testis myotubes in which nascent testis myotubes migrate as a collective onto and along the testis, dependent on FGF-regulated expression of myosin II.

  13. [Intrascrotal malignant mesothelioma diagnosed after surgery for hydrocele testis: a case report].

    Science.gov (United States)

    Kato, Ryuichi; Matsuda, Yohei; Maehana, Takeshi; Miyao, Noriomi; Konishi, Yasuhiro; Kon, Shin-Ichiro

    2012-01-01

    We report here a case of intrascrotal malignant mesothelioma, arising from the tunica vaginalis, which was diagnosed after surgery for hydrocele testis. A 52-year-old man underwent left hydrocelectomy for hydrocele testis. After pathological diagnosis as malignant mesothelioma from the specimen of tunica vaginalis, left radical orchiectomy was performed. The patient had no exposure to asbestos and there has been no evidence of recurrence.

  14. Contralateral testicular biopsy procedure in patients with unilateral testis cancer: is it indicated?

    Science.gov (United States)

    Heidenreich, Axel; Moul, Judd W

    2002-11-01

    About 5% of all patients with unilateral testis cancer harbor testicular intraepithelial neoplasia (TIN) in their contralateral testicle, which will progress into an invasive germ-cell tumor over time. Accurate diagnosis of TIN by a random surgical testis biopsy examination and effective therapy by local radiation has led to the concept of a contralateral screening biopsy procedure in all testis cancer patients. Screening and preventive treatment, however, only are indicated if (1) therapeutic outcome of the screened population is improved and (2) physiologic function of the affected organ might be maintained. Based on a critical review of the literature, some drawbacks of this policy have to be considered and the routine indication for contralateral testis biopsy procedure has to be questioned: (1) all TIN-negative patients still have to undergo meticulous follow-up evaluation for metachronous testis cancer owing to a false-negative biopsy diagnosis rate of 0.3%; (2) testis biopsy procedure is associated with a 15% to 20% complication rate, which might a negative impact on endocrine and exocrine testicular function; (3) local radiation of TIN results in irreversible infertility owing to eradication of spermatogenesis; (4) local radiation of TIN results in an impairment of endocrine Leydig cell function in 25% of patients; (5) therapeutic outcome and prognosis will not be improved in irradiated patients as compared with patients on surveillance; (6) local tumor resection for the management of metachronous testicular cancer represents an effective and viable option. The current literature does not support the strategy to perform contralateral testis biopsy procedures in all patients with unilateral testicular germ-cell tumors. Testis biopsy procedures might, however, be offered to high-risk (34%) patients for contralateral TIN with a testicular volume less than 12 mL, a history of cryptorchidism, and an age less than 30 years. Copyright 2002, Elsevier Science (USA

  15. Cancer/testis antigens and gametogenesis: a review and "brain-storming" session

    OpenAIRE

    Kalejs, Martins; Erenpreisa, Jekaterina

    2005-01-01

    Abstract Genes expressed both in normal testis and in malignancies (Cancer/ Testis associated genes – CTA) have become the most extensively studied antigen group in the field of tumour immunology. Despite this, many fundamentally important questions remain unanswered: what is the connection between germ-cell specific genes and tumours? Is the expression of these genes yet another proof for the importance of genome destabilisation in the process of tumorigenesis?, or maybe activation of these ...

  16. Plastins regulate ectoplasmic specialization via its actin bundling activity on microfilaments in the rat testis

    Directory of Open Access Journals (Sweden)

    Nan Li

    2016-01-01

    Full Text Available Plastins are a family of actin binding proteins (ABPs known to cross-link actin microfilaments in mammalian cells, creating actin microfilament bundles necessary to confer cell polarity and cell shape. Plastins also support cell movement in response to changes in environment, involved in cell/tissue growth and development. They also confer plasticity to cells and tissues in response to infection or other pathological conditions (e.g., inflammation. In the testis, the cell-cell anchoring junction unique to the testis that is found at the Sertoli cell-cell interface at the blood-testis barrier (BTB and at the Sertoli-spermatid (e.g., 8-19 spermatids in the rat testis is the basal and the apical ectoplasmic specialization (ES, respectively. The ES is an F-actin-rich anchoring junction constituted most notably by actin microfilament bundles. A recent report using RNAi that specifically knocks down plastin 3 has yielded some insightful information regarding the mechanism by which plastin 3 regulates the status of actin microfilament bundles at the ES via its intrinsic actin filament bundling activity. Herein, we provide a brief review on the role of plastins in the testis in light of this report, which together with recent findings in the field, we propose a likely model by which plastins regulate ES function during the epithelial cycle of spermatogenesis via their intrinsic activity on actin microfilament organization in the rat testis.

  17. Diffusion-weighted MRI of the testis in hydrocele: a pilot study.

    Science.gov (United States)

    Gulum, Mehmet; Cece, Hasan; Yeni, Ercan; Savas, Murat; Ciftci, Halil; Karakas, Ekrem; Celik, Hakim; Yagmur, Ismail

    2012-01-01

    To investigate the effects of increased pressure due to the accumulated fluid around testis in hydrocele on testis diffusion and whether this effect (if any) is correlated with the amount of fluid and duration of hydrocele. 49 patients with unilateral hydrocele were evaluated by magnetic resonance diffusion-weighted imaging. In the study group, while the apparent diffusion coefficient (ADC) values of the testicles with hydrocele differed significantly before and 3 months after the hydrocelectomy (p = 0.001), the ADC values of the testicles without hydrocele were not statistically different (p = 0.101). There was a significant negative correlation between the ADC values of the testicles with hydrocele and the amount of the liquid aspirated during the hydrocelectomy (r = -0.615, p = 0.001). There was no correlation between the ADC values of testis and the duration of hydrocele (r = -287, p = 0.156). This is supported by the findings of the present study of reduced ADC values of testis, meaning reduced diffusion of the testis, with an increasing amount of fluid. The pressure of the fluid was suggested to show a mechanical effect that plays an important role in the dysfunction of the testis. The possible dysfunctions of the testicular tissue suggest that they may have a negative effect on fertility. Copyright © 2012 S. Karger AG, Basel.

  18. Recombinant FSH versus HP-HMG for controled ovarian stimulation in intracitoplasmic sperm injection cycles.

    Science.gov (United States)

    Petanovski, Zoranco; Dimitrov, Gligor; Aydin, Byrol; Hadzi-Lega, Makjuli; Sotirovska, Valentina; Suslevski, Damjan; Saltirovski, Stefan; Matevski, Vladimir; Stojkovska, Snezana; Lazarova, Ana; Petanovska, Emilija; Balkanov, Trajan

    2011-01-01

    The aim of this study was to make a conclusion about aplicability of two differnet gonadothropins in COS (rFSH versus HP-hMG). The primary conclusion for the success as a result of COS are the mean number of retrived oocytes, mature oocytes, fertilization rate, mean number of quality embrios, and criopreverzed embrios. The secondary conclusions were clinical pregnancy rate and delivery rates. The study was a retrospective case-control study,. A total of 1238 fresh, non donor, IVF cycles with COS were analyzed, but to minimize the bias, only the first cycle for each patient below 40 yaears old, in that period was analyzed. This selection composed the group of respondents that was analyzed which in total amounted to 760 patients.( rFSH = 422, HP-hMG = 338). The patients underwent COS by long luteal protocol using two differnt inducers of COS (rFSH and HP-hMG). The average starting dose of rFSH used was significantely lower (152.7 +/- 41.1IU), whereas with HMG it was (228.8 +/- _68.7 IU, p=000000). The average number of IU gonadothropin used in therapy, statistically highly is significantly lower when r- FSH is used as an inducer. (1639.2 +/- 476.9 IU, rFSH vs 2356.4 +/- 955.1 IU, HP-hMG, p HP-hMG-11.8 +/- 7.1 v 10.7 +/- 6.5, p = 0.028 ; mature oocytes: rFSH v HP-hMG 9.9 +/- 6.2 v8.7 +/- 5.5 p = 0.009). However, we did not find a significant difference in the use of the COS inductors regarding the clinical pregnancy rate (rFSH v HP-hMG 49.5% vs 48.9% p=0.92) and delivery rate (rFSH vs HP-hMG 42.9% vs 43.4% p=0.96).CONCLUSIONSs: Our study showed that rFSH is more powerful and more applicable in individualized dosing then HP-hMG and brings better results from COS (more oocytes, more matured oocytes).

  19. Theoretical study on the polar hydrogen-π (Hp-π) interactions between protein side chains.

    Science.gov (United States)

    Du, Qi-Shi; Wang, Qing-Yan; Du, Li-Qin; Chen, Dong; Huang, Ri-Bo

    2013-01-01

    In the study of biomolecular structures and interactions the polar hydrogen-π bonds (Hp-π) are an extensive molecular interaction type. In proteins 11 of 20 natural amino acids and in DNA (or RNA) all four nucleic acids are involved in this type interaction. The Hp-π in proteins are studied using high level QM method CCSD/6-311 + G(d,p) + H-Bq (ghost hydrogen basis functions) in vacuum and in solutions (water, acetonitrile, and cyclohexane). Three quantum chemical methods (B3LYP, CCSD, and CCSD(T)) and three basis sets (6-311 + G(d,p), TZVP, and cc-pVTZ) are compared. The Hp-π donors include R2NH, RNH2, ROH, and C6H5OH; and the acceptors are aromatic amino acids, peptide bond unit, and small conjugate π-groups. The Hp-π interaction energies of four amino acid pairs (Ser-Phe, Lys-Phe, His-Phe, and Tyr-Phe) are quantitatively calculated. Five conclusion points are abstracted from the calculation results. (1) The common DFT method B3LYP fails in describing the Hp-π interactions. On the other hand, CCSD/6-311 + G(d,p) plus ghost atom H-Bq can yield better results, very close to the state-of-the-art method CCSD(T)/cc-pVTZ. (2) The Hp-π interactions are point to π-plane interactions, possessing much more interaction conformations and broader energy range than other interaction types, such as common hydrogen bond and electrostatic interactions. (3) In proteins the Hp-π interaction energies are in the range 10 to 30 kJ/mol, comparable or even larger than common hydrogen bond interactions. (4) The bond length of Hp-π interactions are in the region from 2.30 to 3.00 Å at the perpendicular direction to the π-plane, much longer than the common hydrogen bonds (~1.9 Å). (5) Like common hydrogen bond interactions, the Hp-π interactions are less affected by solvation effects.

  20. Studies on extraction of uranium by Di-CyclohexylhydrogenPhosphonate (DCyHeHP)

    International Nuclear Information System (INIS)

    Annam, Suresh; Sivaramakrishna, Akella; Vijayakrishna, Kari; Gopakumar, G.; Brahmmananda Rao, C.V.S.; Sivaraman, N.

    2015-01-01

    Monodentate organophosphorous compounds are successfully used for the isolation of uranium and plutonium from the spent nuclear fuel. Applications of monodentate H-phosphonates have increased in the recent past. In the present work, we focus on synthesis, characterization and extraction behavior of dicyclohexylhydrogenphosphonate (DCyHeHP), a cyclic member of H-Phosphonate family towards extraction of uranium from nitric acid medium and to understand the effect of cyclic ring in H-phosphonates for this process. Extraction studies of uranium were carried out by using 0.1M solution of DCyHeHP in xylene at 303K. The extraction ability of the DCyHeHP in xylene was studied and compared with dihexylhydrogen phosphonate (DHeHP), the linear analogue of this compound. DCyHeHP was synthesized by reacting cyclohexanol with phosphorous (III) chloride under solvent free conditions at room temperature for 4 hours and characterized by various spectroscopic techniques like IR, 1 H and 31 P NMR. (author)

  1. P-27/HP endolysin as antibacterial agent for antibiotic resistant Staphylococcus aureus of human infections.

    Science.gov (United States)

    Gupta, Ragini; Prasad, Yogendra

    2011-07-01

    Staphylococcus aureus causes a wide range of suppurative infections in humans and animals. Due to its high virulence, ability to adopt various environmental conditions, and acquired multiple drug resistance, treatment of such infections has become difficult. Therefore, there is an immense need to develop alternate drug modalities to control this pathogen. In past few years, phage-encoded endolysin therapy has emerged as a new hope not only due to its ability to specifically kill the target bacteria irrespective of their antibiotic sensitivity but also because of minimum or no side effects, a problem associated with antibiotic therapy. In this article, we report purification of a broad spectrum anti-staphylococcal endolysin (P-27/HP endolysin) encoded by phage P-27/HP isolated from sewage water. On SDS-PAGE endolysin resolved in three polypeptides of molecular weights 33.5, 48.6, and 62.2 kDa. Endolysin exhibited maximum in vitro lytic activity at temperature between 35 and 40°C and pH 7.0. In vivo experiments revealed considerable (99.9%) elimination of S. aureus 27/HP from spleens of endolysin-treated mice and had saved them from death due to bacteremia caused by S. aureus 27/HP challenge infection. Thus, P-27/HP endolysin offers suitable substitute of antibiotics to control S. aureus infections.

  2. Haptoglobin (HP) polymorphisms and human longevity: a cross-sectional association study in a Central Italy population.

    Science.gov (United States)

    Napolioni, Valerio; Giannì, Paola; Carpi, Francesco M; Concetti, Fabio; Lucarini, Nazzareno

    2011-03-18

    Haptoglobin (HP), which scavenges free, cell-toxic hemoglobin and has anti-inflammatory and immune-modulatory function in extravascular tissues, may represent an excellent candidate gene to investigate the life-span expectancy. HP 1/2 polymorphism has been determined for 1072 (569 females, 503 males) unrelated healthy individuals from Central Italy, 18-106 years old, divided into three gender-specific age classes defined according to demographic information and accounting for the different survivals between sexes. HP*1F/S subtyping was also performed to check the possible existence for a preferential advantage of HP*1F or HP*1S allele. HP*1/*1 genotype results associated to increased probability of young subjects of attaining longevity (Comparison 1: O.R. 1.709, p=0.0114) with a concomitant advantage of HP*1 allele (Comparison 1: O.R. 1.273, p=0.0194). On the other side, carriers of HP*2 allele displayed an overall significant disadvantage in reaching Age Class 2 (O.R. 0.585, p=0.0092). No significant differences were noticed between age groups either considering total HP*1F and HP*1S allele frequencies or according to HP 1/2 genotypes. The crucial role played by HP in aging process is warranted by its many established functions and its related phenotypes so that it may be considered an important gene involved in the determination of human survival. Copyright © 2010 Elsevier B.V. All rights reserved.

  3. Influence of niobium addition on the high temperature mechanical properties of a centrifugally cast HP alloy

    International Nuclear Information System (INIS)

    Andrade, A.R.; Bolfarini, C.; Ferreira, L.A.M.; Vilar, A.A.A.; Souza Filho, C.D.; Bonazzi, L.H.C.

    2015-01-01

    The influence of niobium addition on the mechanical properties at high temperature of HP alloy has been investigated. Two HP alloys were centrifugally cast with a similar chemical composition differing only in the niobium content. Low strain rate high temperature tensile tests and creep-rupture tests were performed in the range of 900–1100 °C, and the results compared between the alloys. According to the results, the high temperature mechanical behavior of both alloys is controlled by several factors like solid solution, network of eutectic carbides, intradendritic precipitation and dendrite spacing. A significant increase in the mechanical properties for the HP alloy with niobium addition was found within the temperature range of 900–1050 °C. Beyond this temperature the mechanical behavior of both alloys is basically the same

  4. Weak Interactions under Pressure: hp-CuBi and Its Analogues.

    Science.gov (United States)

    Guo, Kai; Akselrud, Lev; Bobnar, Matej; Burkhardt, Ulrich; Schmidt, Marcus; Zhao, Jing-Tai; Schwarz, Ulrich; Grin, Yuri

    2017-05-08

    The metastable binary compound hp-CuBi was obtained from the direct chemical reaction between copper and bismuth at a pressure of 5 GPa and a temperature of 720 K. The atomic arrangement comprises slabs of puckered Cu layers sandwiched between Bi planes. The QTAIM charges calculated for compounds of bismuth with transition metals reveal negligible charge transfer for hp-CuBi. Analysis of the chemical bonding with position-space techniques discloses multicenter interactions within the Bi-Cu-Bi slabs and lone-pair interactions of the van der Waals type between these entities. hp-CuBi exhibits metal-type electrical conductivity with superconductivity below T c =1.3 K. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  5. Pathological and immunological characteristics of piglets infected experimentally with a HP-PRRSV TJ strain.

    Science.gov (United States)

    Li, Zhenguang; He, Yanliang; Xu, Xiaoqin; Leng, Xue; Li, Shufen; Wen, Yongjun; Wang, Fengxue; Xia, Mingqi; Cheng, Shipeng; Wu, Hua

    2016-10-12

    Porcine reproductive and respiratory syndrome (PRRS) remains a major threat to swine industry all over the world. The aim of this study was to investigate the mechanism of pathogenesis and immune responses caused by a highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV). All piglets experimentally infected with a HP-PRRSV TJ strain virus developed typical clinical signs of PRRS. The percentages of CD3 + , CD4 + , and CD8 + lymphocytes significantly decreased in the infected group as compared to the uninfected control animals (p HP-PRRSV TJ strain infection would activate host humoral immune response at the early period post infection and cause severe pathological damages on lungs and inhibit cellular immune response after infection.

  6. Endocrine disrupting compounds exposure and testis development in mammals

    Science.gov (United States)

    Egbowona, Biola F.; Mustapha, Olajide A.

    2011-01-01

    In the last few decades, there is substantial evidence that male reproductive function is deteriorating in humans and wildlife and this is associated with unintentional exposure to widely used synthetic chemicals. Subsequently, much has been done to show that certain chemicals in the environment adversely interfere with the developing fetal gonads of the laboratory animals. Some in vitro studies have demonstrated treatment-induced reproductive problems in offspring exposed to endocrine disrupting compounds (EDC) which are similar to those observed in wildlife and human population. Few EDC studies have demonstrated that there are certain periods of gestation when the developing fetus is highly sensitive and at risk of small endocrine changes. Similar observations have been made in the sewage sludge model, however, while animal studies have been insightful in providing valuable information about the range of effects that can be attributed to in utero exposure to EDCs, varying levels of maternal doses administered in different studies exaggerated extrapolation of these results to human. Thus the EDC concentration representative of fetal exposure levels is uncertain because of the complexities of its nature. So far, the level of fetal exposure can only be roughly estimated. There is substantial evidence from animal data to prove that EDCs can adversely affect reproductive development and function in male and more has accumulated on the mechanisms by which they exert their effects. This paper therefore, reviews previous studies to highlight the extent to which testis development can be disrupted during fetal life. PMID:29255381

  7. Mesothelioma of the testis and nephrotic syndrome: a case report

    Directory of Open Access Journals (Sweden)

    Bacchetta Justine

    2009-06-01

    Full Text Available Abstract Introduction Paraneoplastic glomerulopathies are rare manifestations of neoplastic disease to be distinguished from iatrogenic renal damage. Solid tumors are preferentially associated with membranous nephropathy, whereas Hodgkin's lymphomas are associated with minimal change disease. Case presentation We report a 63-year-old Caucasian male diagnosed with a mesothelioma of the tunica vaginalis testis who, secondary to this, also presented with a nephrotic syndrome due to minimal change disease. In the present case, the paraneoplastic etiology of the nephrotic syndrome can be discussed on four unusual elements: minimal change lesions were found; the glomerulopathy was very sensitive to corticosteroids; the nephrotic syndrome occurred 11 months after the diagnosis of the primary malignancy, but concomitantly with the recurrence; and the nephrotic syndrome did not decrease with tumor control and did not recur when the mesothelioma escaped treatment. No other etiologies could nevertheless explain this phenomenon. Conclusion Paraneoplastic nephrotic syndrome is often associated with membranous nephropathy in patients with solid tumors, especially in patients with lung and gastrointestinal tract neoplasia. The management of these patients is associated with a symptomatic treatment such as sodium and water restriction, diuretics and ACE inhibitors and a prophylaxis of specific complications of nephrotic syndrome including thromboembolism, infections and lipid abnormalities. Treatment of neoplasia must be undertaken rapidly, treatments must be regularly analyzed and drugs binding to albumin may be used with precaution.

  8. Widespread expression of SAA and Hp RNA in bovine tissues after evaluation of suitable reference genes.

    Science.gov (United States)

    Lecchi, Cristina; Dilda, Francesca; Sartorelli, Paola; Ceciliani, Fabrizio

    2012-01-15

    The serum amyloid A (SAA) and haptoglobin (Hp) are the most prominent acute phase proteins (APPs) in cow. Liver mainly produces APPs, but extra hepatic expression has also been demonstrated in some tissues. The major aim of the present study was to assess the constitutive SAA and Hp mRNA expression by quantitative PCR (qPCR) in a wide panel of 33 bovine tissues, including gastrointestinal tract, respiratory system, urogenital system, mammary gland, hematopoietic system, central nervous system, eye, thyroid and heart. Normalization of gene expression in different samples requires reference genes, which are stably expressed. Therefore, seven reference genes were investigated (ACTB, GAPDH, HMBS, SDHA, YWHAZ, SF3A1, EEF1A2) and three genes, namely SF3A1, HMBS and ACTB, were selected after assessing their stability with geNorm™ and NormFinder(©) softwares. The qPCR analysis confirmed liver as the principal source of SAA and Hp, but also identified both APPs' mRNA in almost all tissues. The highest expression rate of SAA was found in thyroid, followed by pancreas and submandibulary gland. Hp mRNA expression was detected at high concentration in pancreas and submandibulary gland. The present data indicated a widespread expression of SAA and Hp also in non pathological conditions, thus envisaging a possible role as immunomodulatory and protective molecules. To understand where SAA and Hp come from is the prerequisite to their utilization as Acute Phase Reaction biomarkers. Copyright © 2011 Elsevier B.V. All rights reserved.

  9. Optimising the ISAR-HP to screen efficiently for functional decline in older patients.

    Science.gov (United States)

    de Gelder, J; Haenen, E; Lucke, J A; Klop, H G; Blomaard, L C; Smit, R A J; Mesri, K; de Groot, B; Fogteloo, A J; Anten, S; Blauw, G J; Mooijaart, S P

    2017-11-01

    The Identification of Seniors At Risk-Hospitalised Patients (ISAR-HP) has recently been included in guidelines as a frailty indicator to identify patients for comprehensive geriatric assessment. Previous studies showed that the conventional cut-off score incorrectly classifies a high percentage of patients as high risk. We aimed to optimise the predictive value of ISAR-HP by using different cut-offs in older acutely hospitalised patients. A prospective follow-up study was performed in two Dutch hospitals. Acutely hospitalised patients aged ≥ 70 years were included. Demographics, illness severity parameters, geriatric measurements and the ISAR-HP scores were obtained at baseline. The primary outcome was a combined end point of functional decline or mortality during 90-day follow-up. In total 765 acutely hospitalised older patients were included, with a median age of 79 years, of whom 276 (36.1%) experienced functional decline or mortality. The conventional ISAR-HP cut-off of ≥ 2 assigned 432/765 patients (56.5%) as high risk, with a positive predictive value (PPV) of 0.49 (95%CI 0.45-0.54) and a negative predictive value of 0.81 (95%CI 0.76-0.85). Thus, 51% of those whom the ISAR-HP denoted as high risk did not experience the outcome of interest. Raising the cut-off to ≥ 4 assigned 205/765 patients (26.8%) as high risk, with a marginally increased PPV to 0.55 (95%CI 0.48-0.62). The ISAR-HP with the conventional cut-off of ≥ 2 incorrectly identifies a large group of patients at high risk for functional decline or mortality and raising the cut-off to 4 only marginally improved performance. Caution is warranted to ensure efficient screening and follow-up interventions.

  10. Efficacy of Type 2 PRRSV vaccine against Chinese and Vietnamese HP-PRRSV challenge in pigs.

    Science.gov (United States)

    Lager, Kelly M; Schlink, Sarah N; Brockmeier, Susan L; Miller, Laura C; Henningson, Jamie N; Kappes, Matthew A; Kehrli, Marcus E; Loving, Crystal L; Guo, Baoquing; Swenson, Sabrina L; Yang, Han-Chun; Faaberg, Kay S

    2014-11-12

    Porcine reproductive and respiratory syndrome virus (PRRSV) causes significant reproductive losses in the sow herd and respiratory disease in growing pigs. The virus belongs to the family Arteriviridae and there are two major genotypes. Type 1 is represented by Lelystad virus, the European prototype virus, and Type 2 is represented by the North American prototype virus, VR-2332. Depending on husbandry, immune status of the herd, and virulence of the isolate, the severity of disease and magnitude of economic loss can be variable. Vaccine use is not always successful indicating a lack of cross-protection between vaccine strains and circulating wild-type viruses. To date, there is no clear method to demonstrate if a vaccine confers protection against a specific isolate except for empirical animal studies. In 2006, a new lineage of Type 2 PRRSV emerged in Chinese swine herds that were suffering dramatic losses resulting in those viruses being described as "Highly Pathogenic PRRSV" (HP-PRRSV). Experimental reproduction of severe disease with HP-PRRSV isolates and virus derived from HP-PRRSV clones demonstrated the causal role of this virus. Recently, partial heterologous protection has been reported for Type 1 and Type 2 attenuated PRRSV vaccines against challenge by different Chinese HP-PRRSV isolates providing some hope for reducing economic loss. This paper reports the efficacy of a commercially available Type 2 attenuated vaccine in young pigs against heterologous challenge with a Chinese and Vietnamese HP-PRRSV isolate. When compared to unvaccinated pigs, vaccination decreased the length of viremia and viral titer, diminished the time of high fever and reduced macroscopic lung scores following homologous and heterologous PRRSV challenge. These results demonstrate the potential use of vaccine as an aid in the control of HP-PRRSV outbreaks. Published by Elsevier Ltd.

  11. Bensal HP Attenuates the Inflammatory Response in Hair Shaving Associated Dermatitis.

    Science.gov (United States)

    Ray, Anjana; Mordorski, Breanne; Landriscina, Angelo; Rosen, Jamie; Nosanchuk, Joshua; Friedman, Adam

    2016-07-01

    Shaving is an ubiquitous practice, and cutaneous irritation and inflammation are common sequelae, which may be worsened by underlying skin conditions or poor hair removal techniques. Moisturizing shaving creams and aftershaves are available to help maintain or restore the epidermal barrier; however, many continue to suffer from post-shave redness, itching, and pain. To reduce post-shave inflammation, some products have included botanical and other natural ingredients, which are often favored by consumers. We evaluated Bensal HP, an ointment containing 3% oak bark extract, 3% salicylic acid, and 6% benzoic acid, which has documented anti-inflammatory and antimicrobial properties, in a murine model of shave irritation to determine whether it would be useful in this clinical setting. Shaving dermatitis was simulated using a depilatory agent and electric clippers, and the shaved area was photographed and treated with Bensal HP daily for four days. Compared to untreated controls, mice treated with Bensal HP experienced a visible reduction in skin irritation and inflammation. These findings were mirrored on histology, as Bensal HP-treated areas demonstrated increased epidermal integrity and decreased dermal inflammatory infiltrate compared to untreated skin. Using immunohistochemistry, fewer neutrophils and macrophages were noted, and cytokine analysis also revealed decreased IL-6 in Bensal HP-treated skin at 24 and 96 hours after shaving. These results highlight the potential of Bensal HP as an anti-inflammatory treatment for shave irritation. Given the product's application against a variety of inflammatory and infectious skin disorders, its use against shave irritation may also improve comorbid skin conditions, such as pseudofolliculitis barbae. J Drugs Dermatol. 2016;15(7):836-840.

  12. ANALYSIS POSITIONING OF NOTEBOOK PRODUCED BY HEWLETT PACKARD (HP IN BALI PROVINCE

    Directory of Open Access Journals (Sweden)

    Ni Wayan Sri Ariyani

    2009-12-01

    Full Text Available Nowadays notebook is not a luxurious thing any more, but has been one of  the things that should be obligatorily owned by high school student, university student, and practitioners. The competition among the notebooks has been getting tight. The reason is that they have been produced with almost similar attributes, qualities, and design but different prices. This study aims at (1 identifying the similarity between the notebook produced by Hewlett Packard (hp and those produced by its competitors such as, IBM, Sony, Acer and Toshiba, (2 identifying the consumers perception of the notebook produced by Hewlett Packard (hp and of those produced by its competitors, (3 identifying what is the superiority of the notebook produced by Hewlett Packard (hp compared with the superiority of those produced by its competitors, and (4 identifying what strategy is relevant in strengthening the positioning of the notebook produced by Hewlett Packard (hp. The samples were determined by purposive sampling, and the respondents employed totaled 100 spreading over Denpasar City. To achieve maximum result, Multi Dimensional Scaling (MDS was applied as the instrument of analysis to identify the similarity among the notebooks and Correspondence Analysis (CA was employed as the instrument of analysis to identify the superiority of each variable of every notebook. The result of the study show that the notebook produced by Hewlett Packard (hp is perceived to resemble those produced by IBM, Sony, and Toshiba. This means that the notebook produced by Hewlett Packard (hp competes against those produced by IBM, Sony, and Toshiba, and that it does not compete against that produced by Acer. The notebook produced by Hewlett Packard (hp is superior in the variables of product varieties, post sales, distribution channels, and promotion. The notebook produced by IBM superior in the variable product quality, while that produced by Sony is superior in the variable of product  design

  13. The next step in coastal numerical models: spectral/hp element methods?

    DEFF Research Database (Denmark)

    Eskilsson, Claes; Engsig-Karup, Allan Peter; Sherwin, Spencer J.

    2005-01-01

    In this paper we outline the application of spectral/hp element methods for modelling nonlinear and dispersive waves. We present one- and two-dimensional test cases for the shallow water equations and Boussinesqtype equations – including highly dispersive Boussinesq-type equations.......In this paper we outline the application of spectral/hp element methods for modelling nonlinear and dispersive waves. We present one- and two-dimensional test cases for the shallow water equations and Boussinesqtype equations – including highly dispersive Boussinesq-type equations....

  14. HP-67 calculator programs for thermodynamic data and phase diagram calculations

    Energy Technology Data Exchange (ETDEWEB)

    Brewer, L.

    1978-05-25

    This report is a supplement to a tabulation of the thermodynamic and phase data for the 100 binary systems of Mo with the elements from H to Lr. The calculations of thermodynamic data and phase equilibria were carried out from 5000/sup 0/K to low temperatures. This report presents the methods of calculation used. The thermodynamics involved is rather straightforward and the reader is referred to any advanced thermodynamic text. The calculations were largely carried out using an HP-65 programmable calculator. In this report, those programs are reformulated for use with the HP-67 calculator; great reduction in the number of programs required to carry out the calculation results.

  15. De niet-scrotale testis: huidige standpunten van de Werkgroep Kinderurologie van de Nederlandse Vereniging voor Urologie [Undescended testis: Current views and advice for treatment

    NARCIS (Netherlands)

    Hack, W.W.M.; Sijstermans, K.; Voort-Doedens, L.M. van der; Meijer, R.W.; Heij, H.A.; Delemarre-van de Waal, H.A.; Pierik, F.H.

    2008-01-01

    Eerder dit jaar verschenen in dit tijdschrift drie artikelen gewijd aan de niet-scrotale testis (NST).1-3 Ze hebben tot veel discussie geleid binnen de Werkgroep Kinderurologie (WGKU) van de Nederlandse Vereniging voor Urologie (NVU), hetgeen resulteerde in dit commentaar. Vooral de artikelen van

  16. An overview of the GAGE cancer/testis antigen family with the inclusion of newly identified members

    DEFF Research Database (Denmark)

    Gjerstorff, M F; Ditzel, H J

    2008-01-01

    GAGE cancer/testis antigens are frequently expressed in many different types of cancer, whereas their expression in normal tissues is limited to the germ cells of the immune-privileged organs, testis and ovary. Thus, GAGE proteins may be attractive candidates for immunotherapy of cancer. This rev......GAGE cancer/testis antigens are frequently expressed in many different types of cancer, whereas their expression in normal tissues is limited to the germ cells of the immune-privileged organs, testis and ovary. Thus, GAGE proteins may be attractive candidates for immunotherapy of cancer...

  17. TORSION TESTIS : ROLE OF COLOR DOPPLER : A STUDY OF 50 CASES

    Directory of Open Access Journals (Sweden)

    Anand

    2015-09-01

    Full Text Available BACKGROUND: T orsion testis is one of the catast r ophic conditions in children and young a d u lts. Traditionally the diagnosis was made clinical presentation and suspicion. Critical decision making is essential to save the testis . OBJECTIVE: To study the usefu lness and efficacy of Doppler ultrasound in correctly diagnosing acute scrotal conditions in children and young adults to save the testis and to avoid negative explorations. METHODS: Over a period of two years 50 patients with acute scrotum were admitted i n general surgery department who underwent Doppler ultrasound scrotum and its efficacy in correctly diagnosing the pathology was analysed. RESULTS: 50 patients with age group <25 years were included in study. Scrotal pain was the most frequent presenting s ymptom of acute scrotum (98% followed by Swelling of the hemiscrolum on the involved side present in 86% of the patients. Doppler ultrasound showed torsion of testis in 18 patients. On Scrotal exploration, torsion of spermatic cord was confirmed in 16 pat ients, one patient had torsion of appendix of testis and the other had Epididymo - orchitis. Thus the sensitivity and specificity of Doppler ultrasonography for testicular torsion was 86.9% and 92.6% respectively. 2 patients with equivocal Doppler findings, but strong clinical suspicion of testicular torsion were explored, and testis was found to be torsed in both two patients. Doppler ultrasonography showed Epididymo - orchitis in 22 patients, torsion of testicular appendage in 2 patients, Idiopathic scrotal edema in one, and in 5 pts no significant pathology found. All twenty patients of epididymo - orchitis, two patients of torsion of testicular appendage, and one patient of idiopathic scrotal edema were managed conservatively. At three weeks follow up, all th e patients were free of symptoms. The sensitivity and specificity of Doppler ultrasonography for epididymo - orchitis was 95% and 100% respectively. CONCLUSIONS: color

  18. Transcriptome profiling of testis during sexual maturation stages in Eriocheir sinensis using Illumina sequencing.

    Directory of Open Access Journals (Sweden)

    Lin He

    Full Text Available The testis is a highly specialized tissue that plays dual roles in ensuring fertility by producing spermatozoa and hormones. Spermatogenesis is a complex process, resulting in the production of mature sperm from primordial germ cells. Significant structural and biochemical changes take place in the seminiferous epithelium of the adult testis during spermatogenesis. The gene expression pattern of testis in Chinese mitten crab (Eriocheir sinensis has not been extensively studied, and limited genetic research has been performed on this species. The advent of high-throughput sequencing technologies enables the generation of genomic resources within a short period of time and at minimal cost. In the present study, we performed de novo transcriptome sequencing to produce a comprehensive transcript dataset for testis of E. sinensis. In two runs, we produced 25,698,778 sequencing reads corresponding with 2.31 Gb total nucleotides. These reads were assembled into 342,753 contigs or 141,861 scaffold sequences, which identified 96,311 unigenes. Based on similarity searches with known proteins, 39,995 unigenes were annotated based on having a Blast hit in the non-redundant database or ESTscan results with a cut-off E-value above 10(-5. This is the first report of a mitten crab transcriptome using high-throughput sequencing technology, and all these testes transcripts can help us understand the molecular mechanisms involved in spermatogenesis and testis maturation.

  19. Biometric and Ultrasonographic Evaluation of the Testis of One-humped Camel (Camelus dromedarius

    Directory of Open Access Journals (Sweden)

    Riaz Hussain Pasha, Anas Sarwar Qureshi*, Laeeq Akbar Lodhi1 and Huma Jamil1

    2011-04-01

    Full Text Available Twenty four adult clinically healthy one-humped male camels (Camelus dromedarius were examined three times (beginning, mid and end in each season (winter, spring, summer and autumn for establishing the normal ultrasonic appearance and seasonal changes in the testicular parenchyma in the natural ecology of Punjab, Pakistan. The testes of each camel were scanned by using a B-mode real time ultrasound scanner fitted with a 7.5-MHz linear-array transducer. Scrotal biometry was done with the measuring tape during all the seasons of year. The tunics of the testes appeared as hyperechoic lines surrounding the homogenous, moderately echogenic parenchyma of the testis. The mediastinum testis was visualized as hyperechoic central line and a spot, in longitudinal and transverse sections, respectively. During winter season, the parenchyma was hyperechoic and mediastinum testis was seen as thin hyperechoic line. In spring, the echogenicity of parenchyma was moderate and mediastinum appeared relatively thick central hyperechoic line. In summer and autumn, less echoic parenchyma and thick band of mediastinum was recorded. Biometric studies showed significantly (P<0.01 higher scrotal length and width of the testis during winter and spring season as compared to summer and autumn. Present study revealed that the ultrasonic structure of camel testis resembles other mammals and season has an apparent effect on the testicular size and echogenicity of the testicular parenchyma in the one-humped camel.

  20. Vulnerability of Prepubertal Mice Testis to Iron Induced Oxidative Dysfunctions In Vivo and Functional Implications

    Directory of Open Access Journals (Sweden)

    Thyagaraju BM

    2008-01-01

    Full Text Available Background: The present study describes the susceptibility of prepubertal testis of mice toprooxidant induced oxidative impairments both under in vitro and in vivo exposure conditions.Materials and Methods: Following in vitro exposure to iron (5,10 and 25 M, oxidative responsemeasured in terms of lipid peroxidation and hydroperoxide levels in testis of pre pubertal mice (4wk was more robust compared to that of pubertal mice (6 wk.Results: Further, in an in vivo study, pre pubertal mice administered (i.p sub lethal doses (12.5,25 and 50mg/100g bw/d, 5d of Iron dextran, showed significant induction of oxidative stressresponse in testis cytosol and mitochondria manifested as lipid peroxidation, generation of reactiveoxygen species, hydroperoxide levels and enhanced protein carbonyl levels (a measure of proteinoxidation. Diminished levels of GSH and total thiols in both cytosol and mitochondria of testissuggested an altered redox state. Significant perturbations in the activities of antioxidant enzymessuch as glutathione transferase, glutathione peroxidase and SOD were discernible suggesting theongoing oxidative stress in vivo. These oxidative impairments were accompanied by functionalimplications in testis as reflected in the altered activities of dehydrogenases and reduced activitiesof both 3 - and 17 -hydroxysteriod dehydrogenase.Conclusion: Collectively, these data provide an account of the susceptibility of prepubertal testisto iron-induced oxidative stress, associated functional consequences and this model is being furtherexploited for understanding the implications on the physiology of testis and consequent effect onfertility.

  1. Trace elemental analysis in cancer-afflicted tissues of penis and testis by PIXE technique

    International Nuclear Information System (INIS)

    Naga Raju, G.J.; John Charles, M.; Bhuloka Reddy, S.; Sarita, P.; Seetharami Reddy, B.; Rama Lakshmi, P.V.B.; Vijayan, V.

    2005-01-01

    PIXE technique was employed to estimate the trace elemental concentrations in the biological samples of cancerous penis and testis. A 3 MeV proton beam was employed to excite the samples. From the present results it can be seen that the concentrations of Cl, Fe and Co are lower in the cancerous tissue of the penis when compared with those in normal tissue while the concentrations of Cu, Zn and As are relatively higher. The concentrations of K, Ca, Ti, Cr, Mn, Br, Sr and Pb are in agreement within standard deviations in both cancerous and normal tissues. In the cancerous tissue of testis, the concentrations of K, Cr and Cu are higher while the concentrations of Fe, Co and Zn are lower when compared to those in normal tissue of testis. The concentrations of Cl, Ca, Ti and Mn are in agreement in both cancerous and normal tissues of testis. The higher levels of Cu lead to the development of tumor. Our results also support the underlying hypothesis of an anticopper, antiangiogenic approach to cancer therapy. The Cu/Zn ratios of both penis and testis were higher in cancer tissues compared to that of normal

  2. Trace elemental analysis in cancer-afflicted tissues of penis and testis by PIXE technique

    Energy Technology Data Exchange (ETDEWEB)

    Naga Raju, G.J. [Swami Jnanananda Laboratories for Nuclear Research, Andhra University, Visakhapatnam 530 003 (India); John Charles, M. [Swami Jnanananda Laboratories for Nuclear Research, Andhra University, Visakhapatnam 530 003 (India); Bhuloka Reddy, S. [Swami Jnanananda Laboratories for Nuclear Research, Andhra University, Visakhapatnam 530 003 (India)]. E-mail: sbr_r@yahoo.com; Sarita, P. [Swami Jnanananda Laboratories for Nuclear Research, Andhra University, Visakhapatnam 530 003 (India); Seetharami Reddy, B. [Swami Jnanananda Laboratories for Nuclear Research, Andhra University, Visakhapatnam 530 003 (India); Rama Lakshmi, P.V.B. [Pathology Department, Andhra Medical College, Visakhapatnam 530 002 (India); Vijayan, V. [Institute of Physics, Sachivalaya Marg, Bhubaneswar 751 001 (India)

    2005-04-01

    PIXE technique was employed to estimate the trace elemental concentrations in the biological samples of cancerous penis and testis. A 3 MeV proton beam was employed to excite the samples. From the present results it can be seen that the concentrations of Cl, Fe and Co are lower in the cancerous tissue of the penis when compared with those in normal tissue while the concentrations of Cu, Zn and As are relatively higher. The concentrations of K, Ca, Ti, Cr, Mn, Br, Sr and Pb are in agreement within standard deviations in both cancerous and normal tissues. In the cancerous tissue of testis, the concentrations of K, Cr and Cu are higher while the concentrations of Fe, Co and Zn are lower when compared to those in normal tissue of testis. The concentrations of Cl, Ca, Ti and Mn are in agreement in both cancerous and normal tissues of testis. The higher levels of Cu lead to the development of tumor. Our results also support the underlying hypothesis of an anticopper, antiangiogenic approach to cancer therapy. The Cu/Zn ratios of both penis and testis were higher in cancer tissues compared to that of normal.

  3. [Construction of a yeast two-hybrid cDNA library from the human testis].

    Science.gov (United States)

    Zheng, Ying; Zhang, Lu-Ping; Jia, Xiao-Qin; Wang, Hai-Yan

    2012-04-01

    To construct a human testis cDNA library for yeast two-hybrid screening. Human normal testis mRNA was purified from total RNA, and ds cDNA was synthesized and amplified using primers SMART III and CDS III oligo (dT) as the base of recombination. The purified PCR products and linearized plasmid pGADT7-Rec were co-transformed into the competent yeast Y187 and recombined by yeast homologous recombinase in the yeast cells to form an active cyclic plasmid. All the clones growing on the SD/-Leu plates were harvested to constitute a human testis cDNA library. We constructed a human testis cDNA library with high multiplication and adequate capacity, from which 2.0 x 10(6) recombinants were obtained. The amplified PCR fragments were between 0.3 kb and 4.0 kb in length. The yeast two-hybrid cDNA library of human testis was successfully constructed by the Clontech SMART method, which has prepared a ground for further studies on the molecular mechanism of spermatogenesis.

  4. TGF-β superfamily signaling in testis formation and early male germline development.

    Science.gov (United States)

    Young, Julia C; Wakitani, Shoichi; Loveland, Kate L

    2015-09-01

    The TGF-β ligand superfamily contains at least 40 members, many of which are produced and act within the mammalian testis to facilitate formation of sperm. Their progressive expression at key stages and in specific cell types determines the fertility of adult males, influencing testis development and controlling germline differentiation. BMPs are essential for the interactive instructions between multiple cell types in the early embryo that drive initial specification of gamete precursors. In the nascent foetal testis, several ligands including Nodal, TGF-βs, Activins and BMPs, serve as key masculinizing switches by regulating male germline pluripotency, somatic and germline proliferation, and testicular vascularization and architecture. In postnatal life, local production of these factors determine adult testis size by regulating Sertoli cell multiplication and differentiation, in addition to specifying germline differentiation and multiplication. Because TGF-β superfamily signaling is integral to testis formation, it affects processes that underlie testicular pathologies, including testicular cancer, and its potential to contribute to subfertility is beginning to be understood. Copyright © 2015 Elsevier Ltd. All rights reserved.

  5. Epididymal anomalies in boys with undescended testis or hydrocele: Significance of testicular location.

    Science.gov (United States)

    Kim, Sun-Ouck; Na, Seong Woong; Yu, Ho Song; Kwon, Dongdeuk

    2015-10-24

    Epididymal anomalies and patent processus vaginalis are frequently found in boys with cryptorchidism or hydrocele. We conducted this study to evaluate the association between epididymal anomalies and testicular location or patent processus vaginalis in boys with undescended testis or hydrocele. Children undergoing surgery with undescended testis (group A, 136 boys and 162 testes) or communicating hydrocele (group B, 93 boys and 96 testes) were included. Testicular locations and epididymal anomalies were investigated prospectively. An anomalous epididymis was defined as anomalies of epididymal fusion that consisted of loss of continuity between the testis, the epididymis, and the long looping epididymis. The epididymis was considered normal when a normal, firm attachment between the testis, the caput, and the cauda epididymis was present. The mean ages of groups A and B were 24.6 ± 19.7 (range, 8-52 months) and 31.4 ± 20.6 months (range, 10-59 months). The incidence of epididymal anomalies was significantly higher in group A than that in group B (65.4 % vs. 13.5 %, P hydrocele, and that these anomalies were more frequent when undescended testis was at a higher level. These results suggest that testicular location is associated with epididymal anomalies rather than patent processus vaginalis.

  6. Cancer-testis antigen expression in bladder cancer.

    Science.gov (United States)

    Fradet, Yves; Picard, V; Bergeron, A; LaRue, H

    2005-12-01

    To evaluate the potential of cCancer-t/Testis antigens (CTAs) as targets for immunotherapy of bladder cancer, we evaluated the expression of 9 CTA genes or families of genes in normal urothelia, bladder tumours and bladder cancer human bladder tissuescell lines. As expression of most CTAs is controlled by epigenetic mechanisms, we also evaluated the effect of the DNA methylase inhibitor 5-aza-2'-deoxycytidine (5-AZA-DC), and/or theand histone deacetylase inhibitors Trichostatin A (TSA) on their expression in bladder cancer cell lines. Expression of NY-ESO-1/LAGE-1, MAGE-A, MAGE-C1, BAGE, HOM-TES-85, SCP-1, SSX-1, SSX-2 and SSX-4 was analyzed by semi-quantitative RT-PCR and Western blotting on 10 normal urothelia, 23 24 superficial and 223 invasive tumours and on 10 cell lines treated with 5-aza-2'-deoxycytidine (5-AZA-DC) and/or Trichostatin A (TSA). Expression of all CTA genes could be observed in at least 1 tumour except for HOM-TES-85 for which mRNA was never detected. MAGE-A, BAGE and NY-ESO-1/LAGE-1 mRNAs were the most frequently detected, respectively in 5677%, 212% and 89% of superficial and in 6461%, 4139% and 276% of invasive tumours. With the exception of MAGE-A, CTA transcripts were rarely detected in the cell lines. However, expression of all CTA genes, except SCP-1, could be induced at various levels by the drugs and 5-AZA-DC was a much more potent inducer than TSA. These data suggest that immunotherapy of bladder cancer could target CTAs, especially those expressed at higher frequency such as MAGE-A, BAGE and NY-ESO-1/LAGE-1. Moreover, their induction by chemotherapeutic agents such as 5-AZA-DC, provides a potential pretreatment aimed at inducing the immunogenicity of the tumours.

  7. GALILEO: un nuovo server HP SuperDome per il calcolo parallelo al CILEA

    OpenAIRE

    Arlandini, Claudio

    2002-01-01

    Il CILEA potenzia il suo parco macchine per il calcolo parallelo ad alte prestazioni con un server HP Superdome a 64 processori, e 64GB di memoria centrale. Questa acquisizione conferma il CILEA tra le più importanti realtà del supercalcolo europeo.

  8. hp-FEM electromechanical transduction model of ionic polymer metal composites

    Czech Academy of Sciences Publication Activity Database

    Pugal, D.; Šolín, Pavel; Kim, K.; Aabloo, A.

    2014-01-01

    Roč. 260, April (2014), s. 135-148 ISSN 0377-0427 R&D Projects: GA ČR(CZ) GAP102/11/0498 Institutional support: RVO:61388998 Keywords : hp-FEM * Nernst-Planck * Poisson Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering Impact factor: 1.266, year: 2014

  9. On Adjusting the H-P Filter for the Frequency of Observations

    NARCIS (Netherlands)

    Uhlig, H.F.H.V.S.; Ravn, M.

    1997-01-01

    This paper studies how the HP-Filter should be adjusted, when changing the frequency of observations. The usual choices in the literature are to adjust the smoothing parameter by multiplying it with either the square of the observation frequency ratios or simply with the observation frequency. In

  10. Susceptibility of the Tomato Mutant High Pigment-2dg (hp-2dg) to Orobanche spp. Infection

    NARCIS (Netherlands)

    Lopez Raez, J.A.; Charnikhova, T.; Mulder, P.P.J.; Kohlen, W.; Bino, R.J.; Levin, I.; Bouwmeester, H.J.

    2008-01-01

    The consumption of natural products with potential health benefits has been continuously growing, and enhanced pigmentation is of major economic importance in fruits and vegetables. The tomato hp-2dg is an important mutant line that has been introgressed into commercial tomato cultivars marketed as

  11. Susceptibility of the tomato mutant high pigment-2dg (hp-2dg) to Orobanche spp. infection.

    Science.gov (United States)

    López-Ráez, Juan Antonio; Charnikhova, Tatsiana; Mulder, Patrick; Kohlen, Wouter; Bino, Raoul; Levin, Ilan; Bouwmeester, Harro

    2008-08-13

    The consumption of natural products with potential health benefits has been continuously growing, and enhanced pigmentation is of major economic importance in fruits and vegetables. The tomato hp-2 ( dg ) is an important mutant line that has been introgressed into commercial tomato cultivars marketed as lycopene rich tomatoes (LRT) because of their enhanced fruit pigmentation, attributed to higher levels of carotenoids, including lycopene. Strigolactones are signaling compounds that mediate host finding in root parasitic plants and are biosynthetically derived from carotenoids. Considering the high carotenoid content of the hp-2 ( dg ) mutant, we studied its susceptibility to the root parasite Orobanche. In a field experiment, the average number of Orobanche aegyptiaca plants growing on hp-2 ( dg ) was surprisingly significantly reduced compared with its isogenic wild-type counterpart. In vitro assays and LC-MS/MS analysis showed that this reduction was associated with a lower production of strigolactones, which apparently renders the high-carotenoid hp-2 ( dg ) mutant less susceptible to Orobanche.

  12. An hp-adaptive strategy for the solution of the exact kernel curved wire Pocklington equation

    NARCIS (Netherlands)

    D.J.P. Lahaye (Domenico); P.W. Hemker (Piet)

    2007-01-01

    textabstractIn this paper we introduce an adaptive method for the numerical solution of the Pocklington integro-differential equation with exact kernel for the current induced in a smoothly curved thin wire antenna. The hp-adaptive technique is based on the representation of the discrete solution,

  13. Machinability of HP-9-4-20 high-strength steel

    Energy Technology Data Exchange (ETDEWEB)

    Morris, T.O.

    1976-01-01

    Machinability tests were conducted on HP-9-4-20 high-strength steel. This material was found to be easier to machine to close tolerances than the maraging steels when heat treated to a similar yield strength and fracture-toughness level. (auth)

  14. Diagnostic value of 14C urea breath test for Hp-associated peptic ulcer

    International Nuclear Information System (INIS)

    Yuan Jimin; Xiang Zuqiong; Chen Ying; Xiao Shudong; Zhang Darong; Liu Wenzhong

    1998-01-01

    Purpose: To develop a method of 14 C urea breath test and to evaluate the accuracy of the method in clinical diagnosis. Methods: Helicobacter pylori(Hp)-positive patients were defined either by positive bacterial culture or by positive rapid urease test of endoscopic biopsy specimens. 1384 patients with suspected peptic disease were underwent 14 C urea breath test. Of them, 44 patients underwent dynamic test at various intervals to determine the optimal time for expiratory air collection. Results: Dynamic breath test showed that the highest point of 14 C counts was at 30 min after administration of 14 C urea. When a cut-off value of 3.5 was selected, the sensitivity and specificity was of the test for detecting Hp infection 96.7% and 96.5% respectively. Hp positive rate detected by 14 C urea breath test in 1092 adults and 292 children was 50.4% and 81.2%. Conclusions: This study suggests that 14 C urea breath test is a simple and reliable method for detection of Hp infection and is of high sensitivity and specificity

  15. New approach to calculate the true-coincidence effect of HpGe detector

    Energy Technology Data Exchange (ETDEWEB)

    Alnour, I. A., E-mail: aaibrahim3@live.utm.my, E-mail: ibrahim.elnour@yahoo.com [Department of Physics, Faculty of Pure and Applied Science, International University of Africa, 12223 Khartoum (Sudan); Wagiran, H. [Department of Physics, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Skudai,Johor (Malaysia); Ibrahim, N. [Faculty of Defence Science and Technology, National Defence University of Malaysia, Kem Sungai Besi, 57000 Kuala Lumpur (Malaysia); Hamzah, S.; Elias, M. S. [Malaysia Nuclear Agency (MNA), Bangi, 43000 Kajang, Selangor D.E. (Malaysia); Siong, W. B. [Chemistry Department, Faculty of Resource Science & Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak (Malaysia)

    2016-01-22

    The corrections for true-coincidence effects in HpGe detector are important, especially at low source-to-detector distances. This work established an approach to calculate the true-coincidence effects experimentally for HpGe detectors of type Canberra GC3018 and Ortec GEM25-76-XLB-C, which are in operation at neutron activation analysis lab in Malaysian Nuclear Agency (NM). The correction for true-coincidence effects was performed close to detector at distances 2 and 5 cm using {sup 57}Co, {sup 60}Co, {sup 133}Ba and {sup 137}Cs as standard point sources. The correction factors were ranged between 0.93-1.10 at 2 cm and 0.97-1.00 at 5 cm for Canberra HpGe detector; whereas for Ortec HpGe detector ranged between 0.92-1.13 and 0.95-100 at 2 and 5 cm respectively. The change in efficiency calibration curve of the detector at 2 and 5 cm after correction was found to be less than 1%. Moreover, the polynomial parameters functions were simulated through a computer program, MATLAB in order to find an accurate fit to the experimental data points.

  16. 76 FR 10403 - Hewlett Packard (HP), Global Product Development, Engineering Workstation Refresh Team, Working...

    Science.gov (United States)

    2011-02-24

    ... DEPARTMENT OF LABOR Employment and Training Administration [TA-W-73,488] Hewlett Packard (HP), Global Product Development, Engineering Workstation Refresh Team, Working On-Site at General Motors... groups: The Non-Information Technology Business Development Team, the Engineering Application Support...

  17. Directional motion of foreign plasmid DNA to nuclear HP1 foci

    Czech Academy of Sciences Publication Activity Database

    Ondřej, Vladan; Kozubek, Stanislav; Lukášová, Emilie; Falk, Martin; Matula, Pa.; Matula, Pe.; Kozubek, M.

    2006-01-01

    Roč. 14, č. 5 (2006), s. 505-514 ISSN 0967-3849 R&D Projects: GA ČR(CZ) GA202/02/0804; GA AV ČR(CZ) IAA1065203 Institutional research plan: CEZ:AV0Z50040507 Keywords : heterochromatin * plastid * HP1 protein Subject RIV: BO - Biophysics Impact factor: 3.057, year: 2006

  18. On optimal node and polynomial degree distribution in one-dimensional hp -FEM

    Czech Academy of Sciences Publication Activity Database

    Chleboun, J.; Šolín, Pavel

    2013-01-01

    Roč. 95, č. 1 (2013), s. 75-88 ISSN 1436-5057 R&D Projects: GA ČR GAP105/10/1682 Institutional support: RVO:61388998 Keywords : hp * boundary value problem * optimal mesh Subject RIV: JM - Building Engineering

  19. Helicobacter pylori HP1034 (ylxH) is required for motility

    NARCIS (Netherlands)

    van Amsterdam, Karin; van der Ende, Arie

    2004-01-01

    Background. Helicobacter pylori motility is essential for the colonization and persistence in the human gastric mucosa. So far, more than 50 genes have been described to play a role in flagellar biosynthesis. H. pylori YlxH (HP1034) is annotated as an ATP-binding protein. However, H. pylori YlxH

  20. Adaptive hp-FEM with Arbitrary-Level Hanging Nodes for Maxwell’s Equations

    Czech Academy of Sciences Publication Activity Database

    Šolín, Pavel; Dubcová, Lenka; Doležel, Ivo

    2010-01-01

    Roč. 2, č. 4 (2010), s. 518-532 ISSN 2070-0733 Institutional research plan: CEZ:AV0Z20570509 Keywords : hp-FEM * arbitrary-level hanging nodes * irregular meshes Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering Impact factor: 0.510, year: 2010 http://www.global-sci.org/aamm/

  1. By die uittrede van Prof. H.P. Wolmarans | Oosthuizen | HTS ...

    African Journals Online (AJOL)

    HTS Teologiese Studies / Theological Studies. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 15, No 2-4 (1959) >. Log in or Register to get access to full text downloads. Username, Password, Remember me, or Register. By die uittrede van Prof. H.P. Wolmarans.

  2. Space-time adaptive hp-FEM for problems with traveling sharp fronts

    Czech Academy of Sciences Publication Activity Database

    Šolín, Pavel; Korous, L.

    2013-01-01

    Roč. 95, č. 1 (2013), S709-S722 ISSN 0010-485X Institutional support: RVO:61388998 Keywords : adaptive hp-FEM * transient problems * sharp fronts Subject RIV: BA - General Mathematics Impact factor: 1.055, year: 2013

  3. One step ahead of technology Cern and HP - an ideal team project

    CERN Multimedia

    Garvey, Kelsey

    2009-01-01

    "In 2004, the It team at Cern recognized the comprehensiveness and future of the LHC Computing Grid Project and realized that the IT infrastructures were not sufficient enough to manage the data. Cern therefore partnered with HP ProCurve the following year to minimize the day-to-day workload and improve infrastructure for data output" (1 page)

  4. Genome-wide analysis reveals the extent of EAV-HP integration in domestic chicken.

    Science.gov (United States)

    Wragg, David; Mason, Andrew S; Yu, Le; Kuo, Richard; Lawal, Raman A; Desta, Takele Taye; Mwacharo, Joram M; Cho, Chang-Yeon; Kemp, Steve; Burt, David W; Hanotte, Olivier

    2015-10-14

    EAV-HP is an ancient retrovirus pre-dating Gallus speciation, which continues to circulate in modern chicken populations, and led to the emergence of avian leukosis virus subgroup J causing significant economic losses to the poultry industry. We mapped EAV-HP integration sites in Ethiopian village chickens, a Silkie, Taiwan Country chicken, red junglefowl Gallus gallus and several inbred experimental lines using whole-genome sequence data. An average of 75.22 ± 9.52 integration sites per bird were identified, which collectively group into 279 intervals of which 5 % are common to 90 % of the genomes analysed and are suggestive of pre-domestication integration events. More than a third of intervals are specific to individual genomes, supporting active circulation of EAV-HP in modern chickens. Interval density is correlated with chromosome length (P < 2.31(-6)), and 27 % of intervals are located within 5 kb of a transcript. Functional annotation clustering of genes reveals enrichment for immune-related functions (P < 0.05). Our results illustrate a non-random distribution of EAV-HP in the genome, emphasising the importance it may have played in the adaptation of the species, and provide a platform from which to extend investigations on the co-evolutionary significance of endogenous retroviral genera with their hosts.

  5. Critical Success Factors in Crafting Strategic Architecture for E-Learning at HP University

    Science.gov (United States)

    Sharma, Kunal; Pandit, Pallvi; Pandit, Parul

    2011-01-01

    Purpose: The purpose of this paper is to outline the critical success factors for crafting a strategic architecture for e-learning at HP University. Design/methodology/approach: A descriptive survey type of research design was used. An empirical study was conducted on students enrolled with the International Centre for Distance and Open Learning…

  6. 75 FR 34169 - Hewlett-Packard Company, Inkjet Consumer Solutions, HP Consumer Hardware Inkjet Lab, Including...

    Science.gov (United States)

    2010-06-16

    ... Hardware Inkjet Lab, Including Leased Workers From Hightower Technology Capital, Inc., Syncro Design, VMC, PDG Oncore, K Force, Supply Source, Sigma Design, Novo Engineering, Act, Stilwell Baker, and... Company, Inkjet Consumer Solutions, HP Consumer Hardware Inkjet Lab, Vancouver, Washington. The notice was...

  7. [Relationships between the enrichment of ETBF, Fn, Hp in intestinal and colorectal cancer].

    Science.gov (United States)

    Zhang, J; Lu, X L; Zhao, G; Shi, H T; Geng, Y; Zhong, W T; Dong, L

    2018-02-23

    Objective: To explore relationships between the enrichment of ETBF, Fn, Hp in feces, tissues and colorectal cancer. Methods: Feces, lesion tissue and adjacent tissue from 24 patients with colorectal cancer and 31 patients with adenomas were collected, and we collected Feces and tissue of 20 healthy control persons. Then the copy numbers of enterotoxigenic B. fragilis (ETBF), Fusobacterium nucleatum (Fn) and Helicobacter pylori (Hp) were determined by quantitative real-time PCR. Immunohistochemical method was used to examine the expression intensity of EGFR and p53, and the relationships between different expression intensity of EGFR, p53 and the numbers of three bacterias. Results: In the feces, copy numbers of ETBF and Fn were as follous: colorectal cancer group>adenomas group>healthy control group ( P healthy control group ( P healthy control group ( P adenomas group>healthy control group ( P healthy control group ( P healthy control group ( P bacteria in the lesion tissue and the adjacent tissue had no significant difference. This happened both in colorectal cancer group and adenomas group. The different expression intensity of EGFR, p53 and the number of three bacteria showed no obviously statistical correlation( P >0.05). Conclusion: Adenomatous polyp and colorectal cancer patients show high enrichment of ETBF, Fn and Hp in both feces and tissues. ETBF, Fn and Hp probably contribute to the development of adenomatous polyp and colorectal cancer. Trial registration Chinese Clinical Trial Registry, ChiCTR-BOC-17012509.

  8. Developing Consensus on the CompHP Professional Standards for Health Promotion in Europe

    Science.gov (United States)

    Speller, Viv; Parish, Richard; Davison, Heather; Zilnyk, Anna

    2012-01-01

    Building on the CompHP Core Competencies for health promotion the Professional Standards for Health Promotion have been developed and consulted on across Europe. The standards were formulated to fit within the complexity of professional, occupational and educational standards frameworks in Europe as learning outcome standards with performance…

  9. A sensitization effect of hematoporphyrin oligomer (HpO) and caffeine for X-ray radiation of skin cancer

    International Nuclear Information System (INIS)

    Ishiguro, Kazumori; Ueda, Keiichi; Miyoshi, Norio; Nakanishi, Kazuo; Fukuda, Masaru

    1990-01-01

    Human malignant melanoma cells (G-361) were irradiated with X-ray for different irradiation times. The amount of DNA damage was much increased by the treatment of the cells with hematoporphyrin oligomer (HpO) before X-ray irradiation. Furthermore, it was found that the caffeine inhibited effectively the repair of the damaged DNA. Furthermore, HpO was administered before X-ray irradiation of transplantable skin tumor in C3H mice and/or caffeine was to be given the mouse after X-ray irradiation. The growth of the irradiated tumor combined with HpO and/or caffeine was more inhibited, and the necrotic area was more expanded than that of the tumor irradiated with X-ray only. Labeling Index utilizing bromodeoxyuridine (BrdU) in the viable area of the treated tumor decreased when HpO and/or caffeine were administered. The amount of DNA damage more increased in the irradiated tumor cells given HpO than the only irradiated cells. And, the repair of the damaged DNA was more delayed in the irradiated tumor cells combined with HpO and caffeine than those with other treatments. In conclusion, it was considered that HpO might be a radioactive sensitizer and caffeine enhanced the inhibition of the repair of DNA damaged. We hope that the treatment of X-ray radiation combined with HpO and caffeine is useful for clinical treatment of human malignant tumor. (author) 54 refs

  10. The Aurora A-HP1γ pathway regulates gene expression and mitosis in cells from the sperm lineage.

    Science.gov (United States)

    Leonard, Phoebe H; Grzenda, Adrienne; Mathison, Angela; Morbeck, Dean E; Fredrickson, Jolene R; de Assuncao, Thiago M; Christensen, Trace; Salisbury, Jeffrey; Calvo, Ezequiel; Iovanna, Juan; Coddington, Charles C; Urrutia, Raul; Lomberk, Gwen

    2015-05-29

    HP1γ, a well-known regulator of gene expression, has been recently identified to be a target of Aurora A, a mitotic kinase which is important for both gametogenesis and embryogenesis. The purpose of this study was to define whether the Aurora A-HP1γ pathway supports cell division of gametes and/or early embryos, using western blot, immunofluorescence, immunohistochemistry, electron microscopy, shRNA-based knockdown, site-directed mutagenesis, and Affymetrix-based genome-wide expression profiles. We find that the form of HP1γ phosphorylated by Aurora A, P-Ser83 HP1γ, is a passenger protein, which localizes to the spermatozoa centriole and axoneme. In addition, disruption in this pathway causes centrosomal abnormalities and aberrations in cell division. Expression profiling of male germ cell lines demonstrates that HP1γ phosphorylation is critical for the regulation of mitosis-associated gene expression networks. In female gametes, we observe that P-Ser83-HP1γ is not present in meiotic centrosomes of M2 oocytes, but after syngamy, it becomes detectable during cleavage divisions, coinciding with early embryonic genome activation. These results support the idea that phosphorylation of HP1γ by Aurora A plays a role in the regulation of gene expression and mitotic cell division in cells from the sperm lineage and in early embryos. Combined, this data is relevant to better understanding the function of HP1γ in reproductive biology.

  11. HP-9810A calculator programs for plotting the 2-dimensional motion of cyclindrical payloads relative to the shuttle orbiter

    Science.gov (United States)

    Wilson, S. W.

    1976-01-01

    The HP-9810A calculator programs described provide the capability to generate HP-9862A plotter displays which depict the apparent motion of a free-flying cyclindrical payload relative to the shuttle orbiter body axes by projecting the payload geometry into the orbiter plane of symmetry at regular time intervals.

  12. A parallel direct solver for the self-adaptive hp Finite Element Method

    KAUST Repository

    Paszyński, Maciej R.

    2010-03-01

    In this paper we present a new parallel multi-frontal direct solver, dedicated for the hp Finite Element Method (hp-FEM). The self-adaptive hp-FEM generates in a fully automatic mode, a sequence of hp-meshes delivering exponential convergence of the error with respect to the number of degrees of freedom (d.o.f.) as well as the CPU time, by performing a sequence of hp refinements starting from an arbitrary initial mesh. The solver constructs an initial elimination tree for an arbitrary initial mesh, and expands the elimination tree each time the mesh is refined. This allows us to keep track of the order of elimination for the solver. The solver also minimizes the memory usage, by de-allocating partial LU factorizations computed during the elimination stage of the solver, and recomputes them for the backward substitution stage, by utilizing only about 10% of the computational time necessary for the original computations. The solver has been tested on 3D Direct Current (DC) borehole resistivity measurement simulations problems. We measure the execution time and memory usage of the solver over a large regular mesh with 1.5 million degrees of freedom as well as on the highly non-regular mesh, generated by the self-adaptive h p-FEM, with finite elements of various sizes and polynomial orders of approximation varying from p = 1 to p = 9. From the presented experiments it follows that the parallel solver scales well up to the maximum number of utilized processors. The limit for the solver scalability is the maximum sequential part of the algorithm: the computations of the partial LU factorizations over the longest path, coming from the root of the elimination tree down to the deepest leaf. © 2009 Elsevier Inc. All rights reserved.

  13. LEO to GEO (and Beyond) Transfers Using High Power Solar Electric Propulsion (HP-SEP)

    Science.gov (United States)

    Loghry, Christopher S.; Oleson, Steven R.; Woytach, Jeffrey M.; Martini, Michael C.; Smith, David A.; Fittje, James E.; Gyekenyesi, John Z.; Colozza, Anthony J.; Fincannon, James; Bogner, Aimee; hide

    2017-01-01

    Rideshare, or Multi-Payload launch configurations, are becoming more and more commonplace but access to space is only one part of the overall mission needs. The ability for payloads to achieve their target orbits or destinations can still be difficult and potentially not feasible with on-board propulsion limitations. The High Power Solar Electric Propulsion (HP-SEP) Orbital Maneuvering Vehicle (OMV) provides transfer capabilities for both large and small payload in excess of what is possible with chemical propulsion. Leveraging existing secondary payload adapter technology like the ESPA provides a platform to support Multi-Payload launch and missions. When coupled with HP-SEP, meaning greater than 30 kW system power, very large delta-V maneuvers can be accomplished. The HP-SEP OMV concept is designed to perform a Low Earth Orbit to Geosynchronous Orbit (LEO-GEO) transfer of up to six payloads each with 300kg mass. The OMV has enough capability to perform this 6 kms maneuver and have residual capacity to extend an additional transfer from GEO to Lunar orbit. This high deltaV capability is achieved using state of the art 12.5kW Hall Effect Thrusters (HET) coupled with high power roll up solar arrays. The HP-SEP OMV also provides a demonstration platform for other SEP technologies such as advanced Power Processing Units (PPU), Xenon Feed Systems (XFS), and other HET technologies. The HP-SEP OMV platform can be leveraged for other missions as well such as interplanetary science missions and applications for resilient space architectures.

  14. The fine structure of the testis wall in Salamandra salamandra (L.) (Amphibia, Urodela).

    Science.gov (United States)

    Bergmann, M; Greven, H; Schindelmeiser, J

    1982-09-01

    In mature fire salamander testes examined in early spring, smooth muscle cells can as a rule be observed in the connective tissue which underlies the peritoneal epithelium covering the testis. These smooth muscle cells show a variable degree of differentiation that ranges from fibroblast-like cells in the cranial immature parts of the testis to contracted smooth muscle cells with bundles of myofilaments in the caudal (regressive) glandular tissue, indicating a close relationship to the spermatogenic cycle. The monolayered peritoneal epithelium consists of one cell type only, which is extremely flattened in most parts of the testis and columnar in the glandular tissue. The height of the epithelium may partly be due to the non-contracted resp. contracted state of the underlying muscle cells. The muscle cells are possibly involved in sperm discharge.

  15. Lipid peroxidation in kidney and testis tissues in experimental hypothyroidism: the role of zinc.

    Science.gov (United States)

    Baltaci, A K; Mogulkoc, R; Ayyildiz, M; Kafali, E; Koyuncuoglu, T

    2014-01-01

    The aim of the present study was to determine the effect of zinc supplementation and zinc deficiency on the lipid peroxidation in the testis and kidney tissues of rats with experimentally induced hypothyroidism. The experimental Groups were formed as follows: 1 - Control; 2 - Sham-Hypothyroidism; 3 - Hypothyroidism (intraperitoneal administration of 10 mg/kg/day PTU for 4 weeks); 4 - Hypothyroidism + 3 mg/kg/day zinc supplementation (4 weeks); 5 - Hypothyroidism + zinc deficiency (4 weeks). The examination of the study results revealed that hypothyroidism in testis and kidney tissues increased MDA levels and decreased GSH levels (phypothyroidism, however, reduced the increased MDA amount and elevated GSH levels (phypothyroidism, on the other hand, was found to produce the opposite results (phypothyroidism caused lipid peroxidation in kidney and testis tissues. Zinc deficiency together with hypothyroidism made lipid peroxidation more evident, while zinc supplementation significantly inhibited the increased oxidative stress by activating the antioxidant system (Tab. 1, Ref. 24).

  16. Screening for Y microdeletions in men with testicular cancer and undescended testis

    DEFF Research Database (Denmark)

    Bor, Pinar; Hindkjaer, Johnny; Kølvraa, Steen

    2006-01-01

    PURPOSE: To investigate a possible association between testicular cancer or undescended testis and Y microdeletions. METHODS: It was designed as a retrospective clinical study. A total of 225 men with testicular cancer or undescended testis were included to study. Fertile men (n = 200) were...... investigated as a control. Genomic DNA, which was extracted from blood samples were investigated with a fluorescent multiplex PCR protocol for screening for Y microdeletions. RESULTS: A single STS missing was found in eight men; one from the control group (sY153), seven from the patients group. The positive...... cases showed a single STS missing of marker sY153 and sY139 in testicular cancer (6/185) and undescended testis (1/40) patients, respectively. CONCLUSIONS: Since no contiguous, real Y microdeletions were found in the study population, it seems that Y microdeletions are not a likely common etiological...

  17. The structural and functional state of hemomicrocirculatory bed and parenchyma of the testis in varicocele

    Directory of Open Access Journals (Sweden)

    O. E. Khallo

    2015-08-01

    Full Text Available Varicose veins of the spermatic cord and testis membranes play a key role in the development of male infertility. Aim. To study the structural features of the bloodstream and testicular parenchyma in terms of varicose veins of the spermatic cord and testis membranes. Meyhods and results. In 23 testicular preparations taken from men of mature age convoluted seminiferous tubules diameter, number of cells of spermatogenic epithelium and interstitial endocrinocytes nuclei volume were studied using macro- and microscopic morphometric methods. It was established that in varicocele testis volume, diameter of convoluted seminiferous tubules and interstitial endocrinocytes nuclei volume significantly reduced. Conclusion. This indicates the presence of circulatory hypoxia of testicles, which leads to a decrease in the volume of interstitial endocrinocytes nuclei by 31%, thereby reducing their functional properties.

  18. Effects of catuaba cristal® on the testis of wistar rats

    Directory of Open Access Journals (Sweden)

    Karine Moura de Freitas

    2011-10-01

    Full Text Available The aim of this study was to evaluate the possible beneficial effects of Catuaba Cristal® (CC, an alcoholic drink made from wine and Erythroxylum catuaba Ar. Cam on testis. Wistar rats either received CC solution (n=8 or water (n=9. Results showed significant body weight reduction within the CC group, although, no weight changes were observed for liver, kidney, testis, epididymis, seminal vesicle and prostate. The volumetric proportion and volume of interstitial tissue and lymphatic space were reduced in the treated group. In the CC group, although the nuclear volume of Leydig cells (LC decreased, the number of LC per testis increased. These results suggested that CC had no beneficial effect on spermatogenesis of Wistar rats.

  19. Xenografting of sheep testis tissue and isolated cells as a model for preservation of genetic material from endangered ungulates.

    Science.gov (United States)

    Arregui, Lucía; Rathi, Rahul; Megee, Susan O; Honaramooz, Ali; Gomendio, Montserrat; Roldan, Eduardo R S; Dobrinski, Ina

    2008-07-01

    Recovery of germ cells could be an option for preservation of the genetic pool of endangered animals. In immature males, xenografting of testis tissue provides the opportunity to recover sperm from these animals. In adult animals, xenografting has been less successful, but de novo morphogenesis of functional testis tissue from dissociated testis cells could be an alternative. To assess the potential use of these techniques in endangered bovid species, the domestic sheep was used as a model. Testes from 2-week-old lambs were grafted as tissue fragments or cell suspensions into nude mice. Grafts were recovered at 4, 8, 12 and 16 weeks post grafting. For isolated cells, two additional time points at 35 and 40 weeks after grafting were added. In addition, to analyse the possible effect of social stress among mice within a group on the development of the grafts, testis tissue grafts were recovered 13 weeks post grafting from mice housed individually and in groups. Complete spermatogenesis occurred in sheep testis xenografts at 12 weeks, similar to the situation in situ. Isolated sheep testis cells were able to reorganize and form functional testicular tissue de novo. Housing mice individually or in groups did not have any effect on the development of xenografts. Xenografting of testis tissue might be useful to obtain sperm from immature endangered ungulates that die prematurely. Testis tissue de novo morphogenesis from isolated cells could open interesting options to recover germ cells from mature males with impaired spermatogenesis.

  20. Long non-coding RNA expression profiling of mouse testis during postnatal development.

    Directory of Open Access Journals (Sweden)

    Jin Sun

    Full Text Available Mammalian testis development and spermatogenesis play critical roles in male fertility and continuation of a species. Previous research into the molecular mechanisms of testis development and spermatogenesis has largely focused on the role of protein-coding genes and small non-coding RNAs, such as microRNAs and piRNAs. Recently, it has become apparent that large numbers of long (>200 nt non-coding RNAs (lncRNAs are transcribed from mammalian genomes and that lncRNAs perform important regulatory functions in various developmental processes. However, the expression of lncRNAs and their biological functions in post-natal testis development remain unknown. In this study, we employed microarray technology to examine lncRNA expression profiles of neonatal (6-day-old and adult (8-week-old mouse testes. We found that 8,265 lncRNAs were expressed above background levels during post-natal testis development, of which 3,025 were differentially expressed. Candidate lncRNAs were identified for further characterization by an integrated examination of genomic context, gene ontology (GO enrichment of their associated protein-coding genes, promoter analysis for epigenetic modification, and evolutionary conservation of elements. Many lncRNAs overlapped or were adjacent to key transcription factors and other genes involved in spermatogenesis, such as Ovol1, Ovol2, Lhx1, Sox3, Sox9, Plzf, c-Kit, Wt1, Sycp2, Prm1 and Prm2. Most differentially expressed lncRNAs exhibited epigenetic modification marks similar to protein-coding genes and tend to be expressed in a tissue-specific manner. In addition, the majority of differentially expressed lncRNAs harbored evolutionary conserved elements. Taken together, our findings represent the first systematic investigation of lncRNA expression in the mammalian testis and provide a solid foundation for further research into the molecular mechanisms of lncRNAs function in mammalian testis development and spermatogenesis.

  1. Synchronous rhabdomyosarcoma of the testis and kidney: A case report and review of the literature

    Directory of Open Access Journals (Sweden)

    Babatunde M. Duduyemi

    2016-06-01

    Full Text Available Rhabdomyosarcoma is the commonest soft tissue sarcoma in both children and adolescents representing 40% of such tumours in North America and more than 50% in Africa. The involvement of the paratesticular tissue, testis and the kidney are generally rare and more so when it is occurring synchronously. We present a case of 22 year old male with inguinoscrotal swelling, fever and abdominal distention who was diagnosed as having obstructed left inguinoscrotal hernia and a right renal mass. The patient had surgery, and a diagnosis of synchronous rhabdomyosarcoma of the left testis, paratesticular tissue and right kidney was made by histology and immunohistochemistry.

  2. Purification of beta-acetylglucosaminase and beta-galactosidase from ram testis.

    Science.gov (United States)

    Caygill, J C; Roston, C P; Jevons, F R

    1966-02-01

    1. The presence of beta-galactosidase (EC 3.2.1.23) in an acetic acid extract of ram testis is reported. Some properties of the crude enzyme preparation were studied. 2. The purification of beta-acetylglucosaminase (EC 3.2.1.30) and of beta-galactosidase from the ram-testis extract by ammonium sulphate precipitation and chromatography on a CM-cellulose column is described. 3. The final purifications of the separated enzymes achieved were for the beta-acetylglucosaminase 35 times and for the beta-galactosidase 99 times. 4. The possibility of using DEAE-cellulose and Sephadex G-200 to purify the enzymes was investigated.

  3. Endocrine function in patients treated for carcinoma in situ in the testis with irradiation

    DEFF Research Database (Denmark)

    Petersen, Peter Meidahl; Daugaard, Gedske; Rørth, Mikael

    2003-01-01

    CIS is found in the contralateral testis in 5% of the patients with testicular germ cell cancer. The management of CIS in the contralateral testis is important because the majority - if not all - cases of CIS will progress to invasive disease without treatment. It is well documented that testicular...... are sparse. One study has indicated that more radiotherapy with lower doses per fraction could be useful, but more data are needed to confirm this. Endocrine testicular function has been shown to be impaired already before treatment in patients with CIS and is further impaired after testicular irradiation...

  4. [The age factor in bilateral hydrocele diagnosis secondary to a primitive testis lymphoma: case report, diagnosis and therapy].

    Science.gov (United States)

    Spaziani, E; Di Filippo, A; Picchio, M; De Angelis, F; Corazza, S; Narilli, F; Cimino, G

    2012-01-01

    Primitive NHL represents 9% of testis tumours and the 85% is diagnosed in patient older thant 60 years old. Usually pain and a testis mass are the main manifestations. The Authors report the case of a 82 years-old man with bilateral hydrocele causing severe alteration in the deambulation. The cytology did not show blood cells or neoplastic cell in the FNAB. The US showed a complete modification in the testis. The patient underwent to surgery intervention of vaginalis tunic eversion and resection with the aim of control the symptoms. The histology on multiple biopsy of testis and epidydims bilaterally showed a large B-cell lymphoma. The patient was treated with 6 chemotheray cycles (Rituximab, Vincristine, Cyclophosphamide, Prednisone). Bilateral hydrocele is a rare clinical presentation of testis NHL. In this patient, blood and neoplastic cell absence in the cytology made difficult the differential diagnosis between inflammatory pathology and neoplasia. Hystopathologic study and immunologic pattern can make a certain diagnosis.

  5. IMPARARE L’ITALIANO L2/LS CON TESTI TEATRALI

    Directory of Open Access Journals (Sweden)

    Erminia Ardissino

    2010-09-01

    Full Text Available Il saggio tratta dell'impiego di testi teatrali come fonti di esercizi per l'apprendimento dell'italiano L2/LS. Dopo alcune riflessioni teoriche, si presentano sei proposte di lavoro, con relative soluzioni, adatte a studenti del livello B2-C2 del quadro di riferimento. Si tratta di esercizi ricavati da drammi in un unico atto di Pirandello (La morsa e Lumìe di Sicilia, da Verga (un confronto fra Cavalleria rusticana nella forma drammatica e novellistica, e da Tommaso Landolfi (Ombre. Ogni proposta sfrutta una peculiarità del testo teatrale che si costituisce nell'incontro di dialoghi con didascalie. Anzitutto si tratta di comprendere come si costruiscono i personaggi, quindi di vedere la funzione delle didascalie in relazione al testo, infine di riflettere sulle diverse modalità in cui avvengono i dialoghi, includendo le forme di silenzio. Il testo teatrale appare così molto adatto ad esercizi di lingua, perché mette in gioco le capacità interpretative e immaginative degli studenti, li fa discutere e parlare sulla base delle loro intuizioni.   This paper investigates the use of play scripts as inspiration for Italian L2/FL exercises. After a brief discussion on theory, six project proposals and their solutions, suitable for B2-C2 level students, are presented.  These exercises are based on one-act plays by Pirandello (La Morsa and Lumìe di Sicilia, Verga (a comparison between Cavalleria Rusticana in drama and narrative forms and Tommaso Landolfi (Ombre.  Each project focuses on a specific aspect of the script  and is made up of dialogues with captions. After the way the characters are constructed is investigated, then the function of the captions in relation to the texts is considered, and finally students reflect on the different ways the dialogues are presented, including the pauses. Play scripts lend themselves to language exercises, because they encourage students to use their interpretation skills and imaginations to talk about

  6. The protective effect of ischemic preconditioning on rat testis

    Directory of Open Access Journals (Sweden)

    Ciralik Harun

    2007-12-01

    Full Text Available Abstract Background It has been demonstrated that brief episodes of sublethal ischemia-reperfusion, so-called ischemic preconditioning, provide powerful tissue protection in different tissues such as heart, brain, skeletal muscle, lung, liver, intestine, kidney, retina, and endothelial cells. Although a recent study has claimed that there are no protective effects of ischemic preconditioning in rat testis, the protective effects of ischemic preconditioning on testicular tissue have not been investigated adequately. The present study was thus planned to investigate whether ischemic preconditioning has a protective effect on testicular tissue. Methods Rats were divided into seven groups that each contained seven rats. In group 1 (control group, only unilateral testicular ischemia was performed by creating a testicular torsion by a 720 degree clockwise rotation for 180 min. In group 2, group 3, group 4, group 5, group 6, and group 7, unilateral testicular ischemia was performed for 180 min following different periods of ischemic preconditioning. The ischemic preconditioning periods were as follows: 10 minutes of ischemia with 10 minutes of reperfusion in group 2; 20 minutes of ischemia with 10 minutes of reperfusion in group 3; 30 minutes of ischemia with 10 minutes of reperfusion in group 4; multiple preconditioning periods were used (3 × 10 min early phase transient ischemia with 10 min reperfusion in all episodes in group 5; multiple preconditioning periods were used (5, 10, and 15 min early phase transient ischemia with 10 min reperfusion in all episodes in group 6; and, multiple preconditioning periods were used (10, 20, and 30 min early phase transient ischemia with 10 min reperfusion in all episodes in group 7. After the ischemic protocols were carried out, animals were sacrificed by cervical dislocation and testicular tissue samples were taken for biochemical measurements (protein, malondialdehyde, nitric oxide and histological examination

  7. Helicobacter pylori FlhB function: the F1hB C-terminal homologue HP1575 acts as a "Spare part" to permit flagellar export when the HP0770 FlhB(CC) domain is deleted

    OpenAIRE

    Wand, Matthew E.; Sockett, R. Elizabeth; Evans, Katy J.; Doherty, Neil; Sharp, Paul M.; Hardie, Kim R.; Winzer, Klaus

    2006-01-01

    In Helicobacterpylori 26695, a gene annotated HP1575 encodes a putative protein of unknown function which shows significant similarity to part of the C-terminal domain of the flagellar export protein FlhB. In Salmonella enterica, this part (FibB,c) is proteolytically cleaved from the full-length FlhB, a processing event that is required for flagellar protein export and, thus, motility. The role of FlhB (HP0770) and its C-terminal homologue HP1575 was studied in H. pylori using a range of nonp...

  8. Single-molecule kinetic analysis of HP1-chromatin binding reveals a dynamic network of histone modification and DNA interactions.

    Science.gov (United States)

    Bryan, Louise C; Weilandt, Daniel R; Bachmann, Andreas L; Kilic, Sinan; Lechner, Carolin C; Odermatt, Pascal D; Fantner, Georg E; Georgeon, Sandrine; Hantschel, Oliver; Hatzimanikatis, Vassily; Fierz, Beat

    2017-10-13

    Chromatin recruitment of effector proteins involved in gene regulation depends on multivalent interaction with histone post-translational modifications (PTMs) and structural features of the chromatin fiber. Due to the complex interactions involved, it is currently not understood how effectors dynamically sample the chromatin landscape. Here, we dissect the dynamic chromatin interactions of a family of multivalent effectors, heterochromatin protein 1 (HP1) proteins, using single-molecule fluorescence imaging and computational modeling. We show that the three human HP1 isoforms are recruited and retained on chromatin by a dynamic exchange between histone PTM and DNA bound states. These interactions depend on local chromatin structure, the HP1 isoforms as well as on PTMs on HP1 itself. Of the HP1 isoforms, HP1α exhibits the longest residence times and fastest binding rates due to DNA interactions in addition to PTM binding. HP1α phosphorylation further increases chromatin retention through strengthening of multivalency while reducing DNA binding. As DNA binding in combination with specific PTM recognition is found in many chromatin effectors, we propose a general dynamic capture mechanism for effector recruitment. Multiple weak protein and DNA interactions result in a multivalent interaction network that targets effectors to a specific chromatin modification state, where their activity is required. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

  9. Bensal HP Treatment for Burn and Excision Wounds: An In-Vivo Assessment of Wound Healing Efficacy and Immunological Impact.

    Science.gov (United States)

    Rosen, Jamie; Landriscina, Angelo; Ray, Anjana; Tesfa, Lydia; Nosanchuk, Joshua D; Friedman, Adam J

    2015-11-01

    Natural ingredients are of increasing interest within the field of dermatology. Bensal HP, an ointment containing 3% oak bark extract, 3% salicylic acid, and 6% benzoic acid, is believed to be efficacious against a variety of inflammatory and infectious dermatidites. Here we evaluate Bensal HP's ability to influence wound healing, which has yet to be studied in this setting. Bensal HP applied to burn wounds on the dorsal surface of BALB/c mice significantly attenuated wound expansion in the first few days post-injury as compared to controls. Histological analysis mirrored these findings with accelerated maturation of the wound bed and increased collagen deposition by the end of the study period. Cytokine analysis revealed decreased IL-6 and TNFα secretion in the Bensal HP-treated burns as compared to controls. Similarly, excisional wounds treated with Bensal HP demonstrated comparable wound healing as compared to controls with positive histologic features and increased collagen deposition. Furthermore, IL-6 production was attenuated in the Bensal-HP treated wounds at day 3, with no differences appreciated in IL-6 at day 7 or in TNFα at either time point. While Bensal-HP represents a therapeutic strategy to enhance the histologic and immunologic milieu in burn and excisional wounds, further study is needed to fully elucidate the full potential of this treatment.

  10. Identification of a secreted fatty acid and retinol-binding protein (Hp-FAR-1) from Heligmosomoides polygyrus.

    Science.gov (United States)

    Bath, Jennifer L; Robinson, Michael; Kennedy, Malcolm W; Agbasi, Chidimma; Linz, Lucas; Maetzold, Erin; Scheidt, Michael; Knox, Megan; Ram, Daniel; Hein, Jordan; Clark, Colin; Drees, Jeremy

    2009-09-01

    Hp-FAR-1 is a major, secreted antigen of the parasitic nematode Heligmosomoides polygyrus, a laboratory mouse model frequently used to study the cellular mechanisms of chronic helminth infections. The DNA encoding Hp-FAR-1 was recovered by screening a fourth larval (L₄) H. polygyrus cDNA expression library using antibodies raised against L₄ stage excretory/secretory (E/S) proteins. Predictions of secondary structure based on the Hp-FAR-1 amino acid sequence indicated that an alpha-helix predominates in Hp-FAR-1, possibly with some coiled-coil conformation, with no beta-structure. Fluorescence-based ligand binding analysis confirmed that the recombinant Hp-FAR-1 (rHp-FAR-1) binds the fluorescent fatty acid analog 11-((5-[dimethylaminoaphthalene-1-sulfonyl)amino)undecanoic acid (DAUDA), and by competition oleic acid. RT-PCR amplification of the hp-far-1 gene indicated that the gene is transcribed in all parasitic stages of the organism's life cycle. The presence of a secreted FAR protein in the well-defined laboratory model of H. polygyrus provides an excellent model for the further study and analysis of the in vivo role of secreted FAR proteins in parasitism, and supports the mounting evidence that secreted FAR proteins play a major role in nematode parasitism.

  11. Computational investigations on the electronic and structural properties of the unsaturated silylenoid HP=SiLiF.

    Science.gov (United States)

    Qi, Yuhua; Ma, Jing; Xu, Chongjuan; Geng, Bing; He, Maoxia

    2014-05-01

    The structures of unsaturated silylenoid HP=SiLiF were studied by density functional theory at the B3LYP/6-311+G(d,P) level. Four equilibrium structures, the three-membered ring (1), the four-membered ring (2), the "classical" silane (3), and the linear (4) structures, were located. Their energies are in the order of 4 > 3 > 1 > 2. To exploit the stability of HP=SiLiF, the insertions reaction of 2 and HP=Si into C-Cl have been investigated, respectively. The results show that the insertion of HP=Si is more favorable. To compare with the saturated silylenoid, the insertion reaction of H2SiLiF was also investigated. The calculations indicate that the insertion of HP=SiLiF (2) is more favorable. The unsaturated siylenoid HP=SiLiF has similar reaction characters to saturated silylenoid H2SiLiF and silylene HP=Si.

  12. Single-molecule FRET reveals multiscale chromatin dynamics modulated by HP1α.

    Science.gov (United States)

    Kilic, Sinan; Felekyan, Suren; Doroshenko, Olga; Boichenko, Iuliia; Dimura, Mykola; Vardanyan, Hayk; Bryan, Louise C; Arya, Gaurav; Seidel, Claus A M; Fierz, Beat

    2018-01-16

    The dynamic architecture of chromatin fibers, a key determinant of genome regulation, is poorly understood. Here, we employ multimodal single-molecule Förster resonance energy transfer studies to reveal structural states and their interconversion kinetics in chromatin fibers. We show that nucleosomes engage in short-lived (micro- to milliseconds) stacking interactions with one of their neighbors. This results in discrete tetranucleosome units with distinct interaction registers that interconvert within hundreds of milliseconds. Additionally, we find that dynamic chromatin architecture is modulated by the multivalent architectural protein heterochromatin protein 1α (HP1α), which engages methylated histone tails and thereby transiently stabilizes stacked nucleosomes. This compacted state nevertheless remains dynamic, exhibiting fluctuations on the timescale of HP1α residence times. Overall, this study reveals that exposure of internal DNA sites and nucleosome surfaces in chromatin fibers is governed by an intrinsic dynamic hierarchy from micro- to milliseconds, allowing the gene regulation machinery to access compact chromatin.

  13. Fuzzy physical programming for Space Manoeuvre Vehicles trajectory optimization based on hp-adaptive pseudospectral method

    Science.gov (United States)

    Chai, Runqi; Savvaris, Al; Tsourdos, Antonios

    2016-06-01

    In this paper, a fuzzy physical programming (FPP) method has been introduced for solving multi-objective Space Manoeuvre Vehicles (SMV) skip trajectory optimization problem based on hp-adaptive pseudospectral methods. The dynamic model of SMV is elaborated and then, by employing hp-adaptive pseudospectral methods, the problem has been transformed to nonlinear programming (NLP) problem. According to the mission requirements, the solutions were calculated for each single-objective scenario. To get a compromised solution for each target, the fuzzy physical programming (FPP) model is proposed. The preference function is established with considering the fuzzy factor of the system such that a proper compromised trajectory can be acquired. In addition, the NSGA-II is tested to obtain the Pareto-optimal solution set and verify the Pareto optimality of the FPP solution. Simulation results indicate that the proposed method is effective and feasible in terms of dealing with the multi-objective skip trajectory optimization for the SMV.

  14. Conceptual design of 500 to 3000 hp Stirling engines for stationary power generation

    Science.gov (United States)

    Toscano, W. M.; Chandrasehkar, R.; Harvey, A. C.; Lee, K.

    Both near term and far term conceptual designs of a 373 kW (500 hp) to 2237 kW (3000 hp) Stirling engine for stationary power generation have been prepared. The recommended near term conceptual design is modular, consisting of a basic Stirling engine cylinder of 100 kW that is easily adaptable to any type of heat input or machine output. The engine output configuration selected is the single crank, narrow V, multicylinder arrangement in which any number of cylinders, in groups of four or five, provide the desired power rating. For clean fuel combustion, the prevaporized, premixed, combustion method with exhaust gas recirculation is employed. For coal combustion a Wormser Grate two-stage atmospheric fluidized bed combustion system with a high pressure gas circulation loop system is recommended. The predicted overall fuel to electrical energy conversion efficiency varied between 25 and 34 percent, depending on the system configuration.

  15. Modeling of heterogeneous elastic materials by the multiscale hp-adaptive finite element method

    Science.gov (United States)

    Klimczak, Marek; Cecot, Witold

    2018-01-01

    We present an enhancement of the multiscale finite element method (MsFEM) by combining it with the hp-adaptive FEM. Such a discretization-based homogenization technique is a versatile tool for modeling heterogeneous materials with fast oscillating elasticity coefficients. No assumption on periodicity of the domain is required. In order to avoid direct, so-called overkill mesh computations, a coarse mesh with effective stiffness matrices is used and special shape functions are constructed to account for the local heterogeneities at the micro resolution. The automatic adaptivity (hp-type at the macro resolution and h-type at the micro resolution) increases efficiency of computation. In this paper details of the modified MsFEM are presented and a numerical test performed on a Fichera corner domain is presented in order to validate the proposed approach.

  16. Weighted curve-fitting program for the HP 67/97 calculator

    International Nuclear Information System (INIS)

    Stockli, M.P.

    1983-01-01

    The HP 67/97 calculator provides in its standard equipment a curve-fit program for linear, logarithmic, exponential and power functions that is quite useful and popular. However, in more sophisticated applications, proper weights for data are often essential. For this purpose a program package was created which is very similar to the standard curve-fit program but which includes the weights of the data for proper statistical analysis. This allows accurate calculation of the uncertainties of the fitted curve parameters as well as the uncertainties of interpolations or extrapolations, or optionally the uncertainties can be normalized with chi-square. The program is very versatile and allows one to perform quite difficult data analysis in a convenient way with the pocket calculator HP 67/97

  17. Improvement of pulmonary absorption of poorly absorbable macromolecules by hydroxypropyl-β-cyclodextrin grafted polyethylenimine (HP-β-CD-PEI) in rats.

    Science.gov (United States)

    Zhang, Hailong; Huang, Xiaoyan; Sun, Ya; Lu, Guangyou; Wang, Ke; Wang, Zhigang; Xing, Jianfeng; Gao, Yang

    2015-07-15

    Effects of hydroxypropyl-β-cyclodextrin grafted polyethylenimine (HP-β-CD-PEI) including HP-β-CD-PEI600, HP-β-CD-PEI1800, HP-β-CD-PEI10000 on the pulmonary absorption of insulin, calcitonin, 5(6)-carboxyfluorescein (CF) and fluorescein isothiocyanate dextrans (FDs) with various molecular weights (FD4, FD10 and FD70) were examined by an pulmonary absorption study in rats. Pulmonary absorptions of these poorly absorbable drugs were significantly enhanced by HP-β-CD-PEI1800 and HP-β-CD-PEI10000, and HP-β-CD-PEI1800 with the concentration of 5% (w/v) provided maximal absorption enhancing effect on pulmonary absorption of these model drugs. The toxicity study demonstrated that HP-β-CD-PEI did not induce any toxic action to rat pulmonary membranes. In addition, zeta potential of insulin solution changed to positive by addition of various HP-β-CD-PEI, meanwhile, the degree of positive charge was linearly correlated with absorption enhancing effect of HP-β-CD-PEI, suggesting that positive charge of HP-β-CD-PEI might be related to their absorption enhancing mechanisms for enhancing pulmonary absorption of insulin in rats. In conclusion, HP-β-CD-PEI is a potential and safe absorption enhancer for improving absorption of hydrophilic macromolecules especially peptide and protein drugs by pulmonary delivery. Copyright © 2015 Elsevier B.V. All rights reserved.

  18. Modular hp-FEM system HERMES and its application to Maxwell´s equations

    Czech Academy of Sciences Publication Activity Database

    Vejchodský, Tomáš; Šolín, P.; Zítka, M.

    2007-01-01

    Roč. 76, č. 2 (2007), s. 223-228 ISSN 0378-4754. [MODELLING 2005. Plzeň, 04.06.2005-08.06.2005] R&D Projects: GA ČR GP201/04/P021 Institutional research plan: CEZ:AV0Z10190503 Keywords : hp-FEM * time-harmonic Maxwell´s equations * hierarchic higher-order edge elements Subject RIV: BA - General Mathematics Impact factor: 0.738, year: 2007

  19. Arbitrary-Level Hanging Nodes and Automatic Adaptivity in the hp-FEM

    Czech Academy of Sciences Publication Activity Database

    Šolín, Pavel; Červený, Jakub; Doležel, Ivo

    2008-01-01

    Roč. 77, č. 1 (2008), s. 117-132 ISSN 0378-4754 R&D Projects: GA ČR(CZ) GA102/07/0496; GA ČR GA102/05/0629; GA AV ČR IAA100760702 Institutional research plan: CEZ:AV0Z20570509 Keywords : constrained approximation * hanging nodes * hp-FEM Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering Impact factor: 0.930, year: 2008

  20. Discrete maximum principle for Poisson equation with mixed boundary conditions solved by hp-FEM

    Czech Academy of Sciences Publication Activity Database

    Vejchodský, Tomáš; Šolín, P.

    2009-01-01

    Roč. 1, č. 2 (2009), s. 201-214 ISSN 2070-0733 R&D Projects: GA AV ČR IAA100760702; GA ČR(CZ) GA102/07/0496; GA ČR GA102/05/0629 Institutional research plan: CEZ:AV0Z10190503 Keywords : discrete maximum principle * hp-FEM * Poisson equation * mixed boundary conditions Subject RIV: BA - General Mathematics

  1. Discrete conservation of nonnegativity for elliptic problems solved by the hp-FEM

    Czech Academy of Sciences Publication Activity Database

    Šolín, P.; Vejchodský, Tomáš; Araiza, R.

    2007-01-01

    Roč. 76, 1-3 (2007), s. 205-210 ISSN 0378-4754 R&D Projects: GA ČR GP201/04/P021 Institutional research plan: CEZ:AV0Z10190503 Keywords : discrete nonnegativity conservation * discrete Green's function * elliptic problems * hp-FEM * higher-order finite element methods * Poisson equation * numerical experimetns Subject RIV: BA - General Mathematics Impact factor: 0.738, year: 2007

  2. Investigation of the $^{8}$Li($^{2}$H,p)$^{9}$Li Reaction at REX-ISOLDE

    CERN Multimedia

    2002-01-01

    We propose to investigate the $^{8}$Li($^{2}$H,p )$^{9}$Li reaction at REX-ISOLDE. The main aim is to test a recently found discrepancy in extracted spectroscopic factors for this reaction. As a byproduct we will obtain improved data relevant for predictions of the $^{8}$Li(n,$\\gamma$)$^{9}$Li rate in inhomogeneous nucleosynthesis. For the full experiment including beam tuning and background measurements we ask for 13 shifts.

  3. Effect of chlorpyrifos on soil microbial diversity and its biotransformation by Streptomyces sp. HP-11.

    Science.gov (United States)

    Supreeth, M; Chandrashekar, M A; Sachin, N; Raju, N S

    2016-12-01

    The application of pesticides in agricultural fields not only reaches the target pests but also with soil where it interacts with soil microorganisms resulting in change of microbial diversity. Chlorpyrifos (CP) is one such organophosphorous insecticide most widely used against various insects, termites, and beetles throughout the globe. In the present work, the effect of CP on soil microbial population was assessed by the cultivable method. The fertile soil which does not have a history of any pesticide application was treated with 100 and 200 µg/g of CP along with control which received only sterile water and incubated for 1, 7, and 14 days. The soil amended with the insecticide showed decrease in the number of colony forming units (CFU) of bacteria and fungi. However, Streptomyces sp. HP-11 which tolerated high concentration and also inhibited fungal population was further selected for biodegradation studies. After 14 days of incubation in Mineral salt media (MSM), the strain HP-11 biotransformed CP into 3, 5, 6-trichloro-2-pyridinol (TCP) and Diethyl Phosphorothioate (DETP), and its formation was confirmed by the m/z peak of LC-MS analysis, which was later metabolized to unknown polar metabolites. The results obtained highlights that the application of chlorpyrifos favored the Actinomycete growth in the soil, thereby inhibiting other microorganisms and the strain HP-11 harbors metabolic pathway for detoxification of CP and its hydrolysis product TCP into polar metabolites, thus suggesting the strain HP-11 will be a potential bioaugmenting agent for the bioremediation of chlorpyrifos contaminated soil and water.

  4. Substructuring preconditioners for an h-p domain decomposition method with interior penalty mortaring

    KAUST Repository

    Antonietti, P. F.

    2014-05-13

    We propose and study an iterative substructuring method for an h-p Nitsche-type discretization, following the original approach introduced in Bramble et al. Math. Comp. 47(175):103–134, (1986) for conforming methods. We prove quasi-optimality with respect to the mesh size and the polynomial degree for the proposed preconditioner. Numerical experiments assess the performance of the preconditioner and verify the theory. © 2014, Springer-Verlag Italia.

  5. The CompHP core competencies framework for health promotion in Europe.

    Science.gov (United States)

    Barry, Margaret M; Battel-Kirk, Barbara; Dempsey, Colette

    2012-12-01

    The CompHP Project on Developing Competencies and Professional Standards for Health Promotion in Europe was developed in response to the need for new and changing health promotion competencies to address health challenges. This article presents the process of developing the CompHP Core Competencies Framework for Health Promotion across the European Union Member States and Candidate Countries. A phased, multiple-method approach was employed to facilitate a consensus-building process on the development of the core competencies. Key stakeholders in European health promotion were engaged in a layered consultation process using the Delphi technique, online consultations, workshops, and focus groups. Based on an extensive literature review, a mapping process was used to identify the core domains, which informed the first draft of the Framework. A consultation process involving two rounds of a Delphi survey with national experts in health promotion from 30 countries was carried out. In addition, feedback was received from 25 health promotion leaders who participated in two focus groups at a pan-European level and 116 health promotion practitioners who engaged in four country-specific consultations. A further 54 respondents replied to online consultations, and there were a number of followers on various social media platforms. Based on four rounds of redrafting, the final Framework document was produced, consisting of 11 core domains and 68 core competency statements. The CompHP Core Competencies Framework for Health Promotion provides a resource for workforce development in Europe, by articulating the necessary knowledge, skills, and abilities that are required for effective practice. The core domains are based on the multidisciplinary concepts, theories, and research that make health promotion distinctive. It is the combined application of all the domains, the knowledge base, and the ethical values that constitute the CompHP Core Competencies Framework for Health

  6. Identification of testis-relevant genes using in silico analysis from testis ESTs and cDNA microarray in the black tiger shrimp (Penaeus monodon

    Directory of Open Access Journals (Sweden)

    Wongsurawat Thidathip

    2010-08-01

    Full Text Available Abstract Background Poor reproductive maturation of the black tiger shrimp (Penaeus monodon in captivity is one of the serious threats to sustainability of the shrimp farming industry. Understanding molecular mechanisms governing reproductive maturation processes requires the fundamental knowledge of integrated expression profiles in gonads of this economically important species. In P. monodon, a non-model species for which the genome sequence is not available, expressed sequence tag (EST and cDNA microarray analyses can help reveal important transcripts relevant to reproduction and facilitate functional characterization of transcripts with important roles in male reproductive development and maturation. Results In this study, a conventional testis EST library was exploited to reveal novel transcripts. A total of 4,803 ESTs were unidirectionally sequenced and analyzed in silico using a customizable data analysis package, ESTplus. After sequence assembly, 2,702 unique sequences comprised of 424 contigs and 2,278 singletons were identified; of these, 1,133 sequences are homologous to genes with known functions. The sequences were further characterized according to gene ontology categories (41% biological process, 24% molecular function, 35% cellular component. Through comparison with EST libraries of other tissues of P. monodon, 1,579 transcripts found only in the testis cDNA library were identified. A total of 621 ESTs have not been identified in penaeid shrimp. Furthermore, cDNA microarray analysis revealed several ESTs homologous to testis-relevant genes were more preferentially expressed in testis than in ovary. Representatives of these transcripts, homologs of saposin (PmSap and Dmc1 (PmDmc1, were further characterized by RACE-PCR. The more abundant expression levels in testis than ovary of PmSap and PmDmc1 were verified by quantitative real-time PCR in juveniles and wild broodstock of P. monodon. Conclusions Without a genome sequence, a

  7. [Effect of Yiqi Huoxue Qingre Huashi Recipe on the Eradication Rate of Hp in Peptic Ulcer Patients].

    Science.gov (United States)

    Dai, Gao-zhong; Fan, Xian-jing; Tian, Qiu-shi; Zhu, Shi-kai; Zhao, Ke-xue; Shen, Dan-lei

    2015-12-01

    To observe the effect of Yiqi Huoxue Qingre Huashi Recipe (YHQHR, a recipe capable of supplementing qi, activating blood, clearing heat, and dissipating dampness) on ulcer healing and Helicobacter pylori (Hp) eradication rate in Hp positive peptic ulcer patients, and to explore coccoid Hp occurrence in the eradication. Totally 80 Hp positive peptic ulcer patients were assigned to the treatment group and the control groups by random digit table, 40 in each group. All patients received standard triple therapy of Western medicine for 2 successive weeks. Those in the control group additionally took omeprazole enteric coated tablet, 20 mg each time, once per day for 4 successive weeks. Those in the treatment group additionally took YHQHR, twice per day for 6 successive weeks. The ulcer healing was observed and recorded by gastroscope after discontinued medication of 14 days. The effective rate of ulcer healing under endoscope was statistically calculated. Rapid urease test (RUT) was performed in one small piece of tissue from corpora ventriculi and sinuses ventriculi using 14C breathe test (UBT). Gastric juice was collected from the stomach. Hp urease gene amplification test (urea A-PCR) was performed in living tissue from gastric antrum. Results obtained from the above three test methods were recorded and assessed to decide the final eradiation rate. Gastric mucosa tissue was observed under electron microscope,attempting to find non-eradicated Hp, which was further observed. The total curative effect under gastroscope was 97.5% (39/40 cases) in the treatment group, obviously higher than that in the control group (80.0%, 32/40 cases) (P Hp was 75.0% (30/40 cases), obviously better than that of the control group (52.5%, 21/40 cases) (P Hp numbers after treatment was 14C UBT (12), RUT (8), and urea A-PCR (27), respectively. The Hp positive rate detected by 14C UBT and RUT was lower than the Hp positive rate detected by urea A-PCR (P Hp bacteria could be observed under

  8. Characterization of the contributions of Hp-MMP 9 to the serum acute phase protein response of lipopolysaccharide challenged calves.

    Science.gov (United States)

    Hinds, Charles A; Niehaus, Andrew J; Premanandan, Christopher; Rajala-Schultz, Paivi J; Rings, Donald M; Lakritz, Jeffrey

    2014-10-30

    Bovine respiratory disease (BRD) is a costly feature of modern cattle production. Early and accurate detection of BRD may prove useful in the successful management of this disease. The primary objective of the study was to define the time course of covalent complexes of neutrophil, haptoglobin (Hp) and matrix metalloproteinase 9 (Hp-MMP 9) in serum after intravenous lipopolysaccharide (LPS) in comparison to traditional markers. Our hypothesis was that serum concentrations of neutrophil Hp-MMP 9 provides information distinct from traditional acute phase protein markers. To characterize the neutrophil responses to lipopolysaccharide (E. coli; O111:B4; 2.5 μg/kg body weight), nine healthy, Jersey calves (65-82 days of age; 74.5 ± 13.1 kg) were challenged and physiologic parameters, peripheral blood cell counts and serum cortisol (C), Hp-MMP 9, Hp, alpha1-acid glycoprotein (AGP), serum amyloid A (SAA) were obtained starting 24 hours before to 96 hours post-LPS challenge. Physiologic parameters (temperature, pulse, respiratory rate) and attitude assessed at each time point indicated that LPS challenge resulted in rapid onset of depression, tachypnea, leukopenia, neutropenia and lymphopenia within 1 hour. Serum C concentrations were significantly increased by 1 hour post-LPS. Serum Hp-MMP 9 complexes were detectable in serum by 0.5 hours and peaked at 16 h, serum total Hp remained Hp, SAA and AGP remained significantly greater than baseline out to 96 hours post-LPS. The total systemic exposure to traditional makers is significantly greater than from Hp-MMP 9 CONCLUSION: Using a well described model for acute phase protein responses, the data demonstrate that serum neutrophil Hp-MMP 9 complexes appear sooner and decline more rapidly than other acute phase proteins (APP). Since Hp-MMP9 is stored pre-formed, it provides information specifically addressing the LPS-induced activation of bovine neutrophils. Contributions of Hp-MMP 9 to the serum acute phase protein response

  9. Synthesis and Bioassay of Neurogenically Potent Gangliosides DSG-A, Hp-s1 and Their Analogues.

    Science.gov (United States)

    Shelke, Ganesh B; Lih, Yu-Hsuan; Liao, Ying-Ju; Liang, Chih-Wu; Kuo, Tzer-Min; Ko, Ying-Chin; Luo, Shun-Yuan

    2018-03-27

    In the search of a potent candidate for neurotherapy, we designed and synthesized various analogues of ganglioside Hp-s1. The modification includes the change in hydrophobicity by varying the carbon chain length, altering the number of hydrogen bonds, and replacing the anomeric atom. The chemical synthesis was carried out by using various methods and discussed in details. The neuritogenic activities of these analogues are confirmed in a human neuroblastoma cell line SH-SY5Y. A higher activity of ganglioside Hp-s1 analogue on IL-17A transcript upregulation than ganglioside Hp-s1 was found.

  10. Why testes are resistant to hydatidosis: Is blood-testis-barrier ...

    African Journals Online (AJOL)

    There was demonstrable hydatid cyst (protoscoleces and germinative layer) in testes of five rabbits from Group A, but in one rabbit, both testes were normal. In Group B, three out of four rabbits developed peritoneal hydatidosis. The mechanism of testicular resistance to echinococcosis could be due to blood-testis barrier ...

  11. Testis sparing surgery for Leydig cell tumors: New three cases and ...

    African Journals Online (AJOL)

    A. Chaabouni

    www.ees.elsevier.com/afju · www.sciencedirect.com. Testis sparing surgery for Leydig cell tumors: New three cases and review of the current literature. A. Chaabouni. ∗. , K. Chabchoub, N. Rebai, M. Bouacida, M.H. Slimen,. A. Bahloul, M.N. Mhiri. Department of Urology, Habib Bourguiba University Hospital, Majida Boulila ...

  12. The comparative histology of the testis of two cultured clariid species ...

    African Journals Online (AJOL)

    ... and subsequently viewed under light microscope. Our findings showed that the seminiferous tubules of the Clarias gariepinus testis had wider lumen than those found in Heterobranchus bidorsalis. On the other hand the interlobular spaces were extensive in the Heterobranchus bidorsalis than that of Clarias gariepinus.

  13. Venous infarction of the testis - a cause of non-response to ...

    African Journals Online (AJOL)

    Venous infarction of the testis - a cause of non-response to conservative therapy in epididymo-orchitis. ... poorly to conservative measures. Testicular infarction may be one reason tor this complication. Ultrasonography and radionuclide scanning can elucidate the cause. A patient with this complication is described.

  14. Why testes are resistant to hydatidosis: Is blood-testis-barrier ...

    African Journals Online (AJOL)

    Testes are extremely rare sites for echinococcosis. To our knowledge only three cases of testicular hydatid disease have been reported so far. In this laboratory animal model, we studied hydatidosis in rabbit testis. Fourteen male albino rabbits (body weight 2.5-3 kg) were randomized into two groups: Group A (study group), ...

  15. Testicular germ cell cancer despite previous local radiotherapy to the testis.

    Science.gov (United States)

    Dieckmann, K-P; Lauke, H; Michl, U; Winter, E; Loy, V

    2002-06-01

    Testicular intraepithelial neoplasia (TIN, also carcinoma in situ of the testis) is the uniform precursor of testicular germ cell cancer. Local radiotherapy to the testis with dosages of 18-20 Gy has been found to safely eradicate TIN and germ cells, too. Thus, the general assumption is that the development of invasive germ cell tumours can be prevented by this radiotherapy. Herein, we report two patients with one-sided testicular tumour and biopsy-proven contralateral TIN. Both of them developed germ cell neoplasms in the remaining testis although local radiotherapy with 20 Gy had been applied to the testis. One patient developed pure seminoma 7 years after completion of radiotherapy, the other developed a combined tumour consisting of embryonal carcinoma and seminoma after 5 years. Treatment consisted of orchiectomy in each of the cases. Histologically, both had TIN in the testicular tissue surrounding the new growths. Pathogenetically, a small fraction of radioresistent TIN cells overcoming irradiation and progressing to full-blown germ cell cancer in the later course may be the histogenetic clue to explain these unexpected events. Other explanations, though less probable, could be technical radiotherapeutic failure due to targeting problems and a pre-existing radioresistent germ cell tumour in the irradiated testicle.

  16. Protective effects of some fruit juices with low-fat diet on rat testis ...

    African Journals Online (AJOL)

    Protective effects of some fruit juices with low-fat diet on rat testis damaged by carbon tetrachloride: A genetic and histological study. Medhat R. Rehan, Amina M. G. Zedan, Samah A. El-Hashash, Mona A. Farid, Gehan A. El-Shafie ...

  17. Regulation of androgen receptor mRNA and protein in the rat testis by testosterone

    NARCIS (Netherlands)

    L.J. Blok (Leen); J.M.S. Bartlett (John); J. Bolt-de Vries (Joan); A.P.N. Themmen (Axel); A.O. Brinkmann (Albert); G.F. Weinbauer (G.); E. Nieschlag (Eberhard); J.A. Grootegoed (Anton)

    1991-01-01

    markdownabstract__Abstract__ Adult rats were treated with ethane dimethane sulphonate (EDS), an agent that destroys Leydig cells. Within 5 days after EDS treatment, the levels of testosterone (T) in the circulation and in the testis were decreased to very low values, which makes it possible to

  18. Peritubular Contractile Cells in Testis and Epididymis of the Dog, Canis lupus familiaris

    Directory of Open Access Journals (Sweden)

    Gunter F. Egger

    2009-01-01

    Full Text Available Contractile cells surrounding the tubular system of the mammalian testis and epididymis are supposed to contribute to the initial transport of spermatozoa from the testis to epididymis. Testicular peritubular smooth muscle cells have been characterised in detail especially in rodents and humans. The aim of our study was to assess the distribution of peritubular contractile cells of the canine tubuli seminiferi, rete testis channels, ductuli efferentes, and ductus epididymidis by immunohistochemistry and transmission electron microscopy and to classify these cells with respect to their possible physiological function. The entire tubular system of the canine testis and epididymis is surrounded by contractile cells expressing smooth muscle actin, smooth muscle myosin and desmin, which are enveloped, at least partly, by a basal lamina. Some contractile cells of the tubuli seminiferi, rete channels, and efferent ducts and sometimes also single peritubular cells of the ductus epididymidis express vimentin. Contractile cells of seminiferous tubules and efferent ducts represent an intermediate cell type exhibiting characteristics of both smooth muscle cells (SMC and myofibroblasts, those of rete channels stellate myofibroblasts, and those of the ductus epididymidis SMC. Differences in structure and arrangement of the contractile cells between seminiferous tubules, rete channels, efferent ducts, and ductus epididymidis suggest different functions. Myofibroblasts and contractile cells similar to them could be mainly responsible for the maintenance of an appropriate tissue turgor, whereas contraction of SMC of the ductus epididymidis might cause propulsion of spermatozoa by peristaltic waves.

  19. Antioxidant effects of alpha-tocopherol (vitamine E on testis regeneration in busulfan-treated mice

    Directory of Open Access Journals (Sweden)

    Fatemeh Rohnavaz

    2016-09-01

    (35, 40 mg/kg body weight intraperitonealy. The effects of busulfan was examinned on destruction of testis tissue, semen parameters and catalase enzymatic activity compared with control group after 30 days. Finally, fter determining the effective dose of busulfan, Vitamin E was injected to the mice as an antioxidant enzyme  and its effects on semen parameters and testis tissue was examined after one month.  Results: The results showed that oxidative stress was increased in busulfan-treated mice during 30 days. Catalase enzymatic activity decreased significantly in testis of Busulfan-treated mice in comparison with control group. A significant decrease of sperm number was observed according to treatment by Busulfan. After finding the most damaging dose of busulfan (40 mg/kg in destruction of testis tissue, these mice were injected by vitamine E. The results showed that the oxidative stress and percentage of abnormal sperm were decreased in Busulfan-treated mice that exposed a dose of 100 mg/kg vitamin E. The number of sperm and antioxidant activity of catalase was increased. Conclusion: Vitamin E improves a revival of spermatogenesis and improves testicular parameters in these patients. 

  20. Comparative genomic and in situ hybridization of germ cell tumors of the infantile testis

    NARCIS (Netherlands)

    Mostert, M; Rosenberg, C; Stoop, H; Schuyer, M; Timmer, A; Oosterhuis, W; Looijenga, L

    Chromosomal information on germ cell tumors of the infantile testis, ie, teratomas and yolk sac tumors, is limited and controversial. We studied two teratomas and four yolk sac tumors using comparative genomic hybridization (CGH) and in situ hybridization. No chromosomal anomalies were found in the

  1. Testis sparing surgery for Leydig cell tumors: New three cases and ...

    African Journals Online (AJOL)

    A. Chaabouni

    KEYWORDS. Leydig cell tumor;. Organ-sparing surgery;. Radical orchitectomy. Abstract. Introduction: Leydig cells tumors of the testis are uncommon, representing between 1 and 3% of the testicular tumors and for which the natural history and therapy are debated between radical orchitectomy and organ-sparing surgery.

  2. EXPRESSION OF THE SPERMATOGENIC CELL-SPECIFIC GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE (GAPDS) IN RAT TESTIS

    Science.gov (United States)

    The spermatogenic cell-specific variant of glyceraldehyde 3-phosphate dehydrogenase (GAPDS) has been cloned from a rat testis cDNA library and its pattern of expression determined. A 1417 nucleotide cDNA has been found to encode an enzyme with substantial homology to mouse GAPDS...

  3. Hydatid cyst of testis: An unusual presentation of hydatid disease - case report and review of literature

    Directory of Open Access Journals (Sweden)

    Baldev Singh

    2001-01-01

    Full Text Available Hvdatid disease is a parasitic disease usually caused by Echinococcus granulosus. It usually presents with liver involvement and uncommonly lungs and rarely other organs are involved. A rare case of hydatid testis is reported that was provisionally diagnosed as testicular tumor.

  4. Isolation and characterization of all-trans-retinoic acid-responsive genes in the rat testis

    NARCIS (Netherlands)

    Gaemers, I. C.; van Pelt, A. M.; Themmen, A. P.; de rooij, D. G.

    1998-01-01

    By way of differential screening of testis cDNA libraries from vitamin A-deficient (VAD) rats before and after administration of all-trans retinoic acid (ATRA), genes, the transcription of which was influenced by ATRA, were isolated. Most clones with an increased transcription encoded different

  5. Lipoprotein lipase and endothelial lipase in human testis and in germ cell neoplasms

    DEFF Research Database (Denmark)

    Nielsen, J E; Lindegaard, M L; Friis-Hansen, L

    2009-01-01

    The aim of this study was to investigate endothelial lipase (EL, LIPG) and lipoprotein lipase (LPL) mRNA and protein expression in normal human testis and testicular germ cell tumours (GCT). Both EL and LPL were expressed in normal seminiferous tubules and in the interstitial compartment. EL mRNA...

  6. Examination for intratubular germ cell neoplasia at operation for undescended testis in boys

    DEFF Research Database (Denmark)

    Cortes, Dina; Thorup, Jørgen Mogens; Frisch, M

    1994-01-01

    A total of 843 consecutive boys (median age 12.7 years) who had undergone testicular biopsy at operation for undescended testis was followed into adulthood (median age 25.2 years) to examine for testicular germ cell neoplasia. Five cases of testicular germ cell neoplasia were identified, including...

  7. Tumor in undescended intrapelvic testis revealed by supraclavicular lymphadenopathy: a case report and literature review.

    Science.gov (United States)

    Tazi, Mohammed Fadl; Riyach, Omar; Ahsaini, Mustapha; Ahallal, Youness; Khallouk, Abdelhak; El Fassi, Mohammed Jamal; Farih, Moulay Hassan

    2013-04-26

    Testicular cancer is a rare disease. The incidence of testicular cancer in undescended testicles is of 3 to 48 times greater than in the general population. In the developed countries, the existence of undescended testicles in the adult population is rare, due to systematic practice of elective orchidopexy before the second year of life and orchiectomy in post adolescent males with undescended testicles. Despite these prevention measures, there are still some isolated cases of intra-abdominal testicular tumors in adults. We report a case of testicular cancer in cryptorchid testis revealed by supraclavicular lymphadenopathy. We report a case of a 46 year old fertile man with a history of unilateral cryptorchidism who presented with a palpable left supraclavicular mass and absence of the right testicle. On investigations an intrapelvic testis tumor was diagnosed. Laparotomy and complete excision was carried out. The possible association between the undescended testis and cancer transformations is briefly discussed. Testicular cancer in undescended testicles should not be ignored. Only early diagnosis and lower of testis in scrotumprevent such clinical forms.

  8. Histological changes in the testis of rats treated with Alomo Bitter ...

    African Journals Online (AJOL)

    Considering the increasing demand for herbal aphrodisiacs, this study investigates the effect of Alomo bitters on the histology of testis in adult rats. 36 male rats of comparable weight (151.67 ± 2.89 grams) and sizes were involved in this study. The animals were assigned into four groups; a control group (A) and three test ...

  9. Protective effect of ginger on gentamicin-induced apoptosis in testis of rats.

    Science.gov (United States)

    Zahedi, Afshin; Fathiazad, Fatemeh; Khaki, Arash; Ahmadnejad, Behnam

    2012-01-01

    Ginger, the rhizome of Zingiber officinale, is one of the most widely used spices for various foods and as an herbal medicine in Asian countries. It has been shown that ginger has antioxidant power. Gentamicin is an aminoglycoside antibiotic with a very broad spectrum against microbial pathogens, especially the gram-negative. Many studies revealed that gentamicin induces an oxidative stress-status in the testis by increasing free radical formation and lipid peroxidation. The present study was designed to investigate on the effects of Ginger as a natural anioxidant on testis apoptosis after treatment with gentamicin in rats. In order to study the recovery effects of ginger on testis apoptosis after treatment with gentamicin 40 adult Wistar male rats were selected and randomly divided into four groups. Normal salin control (group I) (n=10), gentamicin control (group II), ginger control (group III) and gentamicin + ginger (group IV) each 10 rats. There was observation of negative effect of Gentamicin on testis histology in rats. The results revealed that there was a significant increase in apoptosis in group III when compared with other groups (P<0.05).However, ginger could decrease apoptosis in group IV that received 100mg/kg/rat of Ginger. Regarding the results, it is recommended that administration of ginger with gentamicin might be beneficial in men who receive gentamicin to treat infections.

  10. Protective Effect of Ginger on Gentamicin-Induced Apoptosis in Testis of Rats

    Directory of Open Access Journals (Sweden)

    Afshin Zahedi

    2012-08-01

    Full Text Available Purpose: Ginger, the rhizome of Zingiber officinale, is one of the most widely used spices for various foods and as an herbal medicine in Asian countries. It has been shown that ginger has antioxidant power. Gentamicin is an aminoglycoside antibiotic with a very broad spectrum against microbial pathogens, especially the gram-negative. Many studies revealed that gentamicin induces an oxidative stress-status in the testis by increasing free radical formation and lipid peroxidation. The present study was designed to investigate on the effects of Ginger as a natural anioxidant on testis apoptosis after treatment with gentamicin in rats. Methods: In order to study the recovery effects of ginger on testis apoptosis after treatment with gentamicin 40 adult Wistar male rats were selected and randomly divided into four groups. Normal salin control (group I (n=10, gentamicin control (group II, ginger control (group III and gentamicin + ginger (group IV each 10 rats. There was observation of negative effect of Gentamicin on testis histology in rats. Results: The results revealed that there was a significant increase in apoptosis in group III when compared with other groups (P<0.05.However, ginger could decrease apoptosis in group IV that received 100mg/kg/rat of Ginger. Conclusion: Regarding the results, it is recommended that administration of ginger with gentamicin might be beneficial in men who receive gentamicin to treat infections.

  11. Malignant mesothelioma of the tunica vaginalis testis: A case report and literature review

    Science.gov (United States)

    Zhang, Neng; Fu, Ni; Peng, Su; Luo, Xu

    2017-01-01

    Malignant mesothelioma of the tunica vaginalis testis is an extremely rare tumor without specific clinical manifestations, mainly including hydrocele formation and a painless mass. We herein present the case of a patient with hydrocele of the left testis, without any other complaints. Tunica vaginalis subinvolution was performed, and postoperative pathological examination revealed a malignant mesothelioma arising from the left tunica vaginalis testis. Whole-body positron emission tomography-computed tomography (PET-CT) and subsequent abdominal and pelvic magnetic resonance imaging (MRI) revealed no evidence of local lymphadenopathy. Radical left orchiectomy was performed after the pathological diagnosis. The pathological examination after the second surgery demonstrated that the tumor had invaded the adjacent periorchium and spermatic cord, but there was no evidence of local lymph node metastasis. Pemetrexed and cisplatin were administered at a dose of 900 and 130 mg, respectively, on the first day of a 28-day cycle. After 6 months of therapy, the disease had not progressed on abdominal and pelvic PET-CT and MRI. The patient was still followed up in our urology outpatient clinic at the time of the present report. Although testicular hydrocele is a common and easily diagnosed condition, detailed medical history and physical examination are required. Thus, when clinicians encounter patients with testicular hydrocele, a variety of possible causes must be considered, including testicular or paratesticular tumors, even rare tumors such as mesothelioma of the tunica vaginalis testis. PMID:29285372

  12. Primary Lymphoma of the Testis in Remission for more than Ten ...

    African Journals Online (AJOL)

    A working diagnosis of right hydrocele with differential diagnosis of lymphangioma was made. Invesigations were essentially normal and patient was scheduled for hydrocelectomy but findings at operations were different. RESULTS: At surgery, there was grossly enlarged hard right testis with thickened spermatic cord.

  13. METABOLOMIC EVALUATION OF RAT LIVER AND TESTIS TO CHARACTERIZE THE TOXICITY OF TRIAZOLE FUNGICIDES

    Science.gov (United States)

    The effects of two triazole fungicides, myclobutanil and triadimefon, on endogenous rat metabolite profiles in blood serum, liver, and testis was assessed using proton nuclear magnetic resonance (1H-NMR) spectroscopy. Adult male Sprague-Dawley rats were dosed daily by gavage for...

  14. Simulating the folding of HP-sequences with a minimalist model in an inhomogeneous medium.

    Science.gov (United States)

    Alas, S J; González-Pérez, P P

    2016-01-01

    The phenomenon of protein folding is a fundamental issue in the field of the computational molecular biology. The protein folding inside the cells is performed in a highly inhomogeneous, tortuous, and correlated environment. Therefore, it is important to include in the theoretical studies the medium where the protein folding is developed. In this work we present the combination of three models to mimic the protein folding inside of an inhomogeneous medium. The models used here are Hydrophobic-Polar (HP) in 2D square arrangement, Evolutionary Algorithms (EA), and the Dual Site Bond Model (DSBM). The DSBM model is used to simulate the environment where the HP beads are folded; in this case the medium is correlated and is fractal-like. The analysis of five benchmark HP sequences shows that the inhomogeneous space provided with a given correlation length and fractal dimension plays an important role for correct folding of these sequences, which does not occur in a homogeneous space. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  15. An efficient algorithm for building locally refined hp - adaptive H-PCFE: Application to uncertainty quantification

    Science.gov (United States)

    Chakraborty, Souvik; Chowdhury, Rajib

    2017-12-01

    Hybrid polynomial correlated function expansion (H-PCFE) is a novel metamodel formulated by coupling polynomial correlated function expansion (PCFE) and Kriging. Unlike commonly available metamodels, H-PCFE performs a bi-level approximation and hence, yields more accurate results. However, till date, it is only applicable to medium scaled problems. In order to address this apparent void, this paper presents an improved H-PCFE, referred to as locally refined hp - adaptive H-PCFE. The proposed framework computes the optimal polynomial order and important component functions of PCFE, which is an integral part of H-PCFE, by using global variance based sensitivity analysis. Optimal number of training points are selected by using distribution adaptive sequential experimental design. Additionally, the formulated model is locally refined by utilizing the prediction error, which is inherently obtained in H-PCFE. Applicability of the proposed approach has been illustrated with two academic and two industrial problems. To illustrate the superior performance of the proposed approach, results obtained have been compared with those obtained using hp - adaptive PCFE. It is observed that the proposed approach yields highly accurate results. Furthermore, as compared to hp - adaptive PCFE, significantly less number of actual function evaluations are required for obtaining results of similar accuracy.

  16. Endocrine roles of D-aspartic acid in the testis of lizard Podarcis s. sicula.

    Science.gov (United States)

    Raucci, F; D'Aniello, S; Di Fiore, M M

    2005-12-01

    In the lizard Podarcis s. sicula, a substantial amount of D-aspartate (D-Asp) is endogenous to the testis and shows cyclic changes of activity connected with sex hormone profiles during the annual reproductive phases. Testicular D-Asp content shows a direct correlation with testosterone titres and a reverse correlation with 17beta-estradiol titres. In vivo experiments, consisting of i.p. injections of 2.0 micromol/g body weight of D-Asp or other amino acids, in lizards collected during the three main phases of the reproductive cycle (pre-reproductive, reproductive and post-reproductive period), revealed that the testis can specifically take up and accumulate D-Asp alone. Moreover, this amino acid influences the synthesis of testosterone and 17beta-estradiol in all phases of the cycle. This phenomenon is particularly evident during the pre- and post-reproductive period, when endogenous testosterone levels observed in both testis and plasma were the lowest and 17beta-estradiol concentrations were the highest. D-Asp rapidly induces a fall in 17beta-estradiol and a rise in testosterone at 3 h post-injection in the testis and at 6 h post-injection in the blood. In vitro experiments show that testicular tissue converted L-Asp into D-Asp through an aspartate racemase. D-Asp synthesis was measured in all phases of the cycle, but was significantly higher during the reproductive period with a peak at pH 6.0. The exogenous D-Asp also induces a significant increase in the mitotic activity of the testis at 3 h (P proliferation cell nuclear antigen (PCNA). The effects of D-Asp on the testis appear to be specific since they were not seen in lizards injected with other D- or L-forms of amino acids with known excitatory effects on neurosecretion. Our results suggest a regulatory role for D-Asp in the steroido-genesis and spermatogenesis of the testis of the lizard Podarcis s. sicula.

  17. Notes on HP1 a software package for simulating variably-saturated water flow, heat transport, solute transport, and biogeochemistry in porous media. HP1 Version 2.2

    International Nuclear Information System (INIS)

    Jacques, D.; Simunek, J.

    2010-01-01

    HP1 is a comprehensive modeling tool in terms of processes and reactions for simulating reactive transport and biogeochemical processes in variably-saturated porous media. HP1 results from coupling the water and solute transport model HYDRUS-1D (Simunek et al., 2009a) and PHREEQC-2 (Parkhurst and Appelo, 1999). This note provides an overview of how to set up and execute a HP1 project using version 2.2.002 of HP1 and version 4.13 of the graphical user interface (GUI) of HYDRUS-1D. A large part of this note are step-by-step instructions for selected examples involving mineral dissolution and precipitation, cation exchange, surface complexation and kinetic degradation networks. The implementation of variably-saturated flow conditions, changing boundary conditions, a layered soil profile or immobile water is also illustrated.

  18. Notes on HP1 a software package for simulating variably-saturated water flow, heat transport, solute transport, and biogeochemistry in porous media. HP1 Version 2.2

    Energy Technology Data Exchange (ETDEWEB)

    Jacques, D.; Simunek, J.

    2010-01-15

    HP1 is a comprehensive modeling tool in terms of processes and reactions for simulating reactive transport and biogeochemical processes in variably-saturated porous media. HP1 results from coupling the water and solute transport model HYDRUS-1D (Simunek et al., 2009a) and PHREEQC-2 (Parkhurst and Appelo, 1999). This note provides an overview of how to set up and execute a HP1 project using version 2.2.002 of HP1 and version 4.13 of the graphical user interface (GUI) of HYDRUS-1D. A large part of this note are step-by-step instructions for selected examples involving mineral dissolution and precipitation, cation exchange, surface complexation and kinetic degradation networks. The implementation of variably-saturated flow conditions, changing boundary conditions, a layered soil profile or immobile water is also illustrated.

  19. Unilateral orchidectomy in donkey (Equus asinus: Evaluation of different surgical techniques, histological and morphological changes on remaining testis

    Directory of Open Access Journals (Sweden)

    Haroon Ali Yousef Hussein

    2013-03-01

    Full Text Available Unilateral orchidectomy (UO is required when further breeding potential is important. It is sometimes necessary to remove a single testis in a matured stallion for therapeutic reasons. In this study, twelve donkeys were used to evaluate three techniques of unilateral castration, histological and morphological changes on the remaining testis. Results of the study showed that each of the surgical techniques used had its advantages and disadvantages in comparison with the other two techniques. Therefore the selection among the three techniques depends on the surgeon preferences and the environment in which the unilateral orchidectomy is performed. The volume of the remaining testis recorded at the end of the study was significantly greater than that estimated at the start of the study (p < 0.05. The percentage of sperm motility obtained from the remaining testis was significantly decreased (p < 0.05. Histological examination of the testis in open surgery (group I (where the scrotum was left opened revealed severe hemorrhages, edema and fibrosis. The testis showed degenerative changes in the seminiferous tubules and interstitial orchitis. Histological examination of the testes removed using a closed technique, (in groups II and III where the scrotum wound was sutured, revealed hyperplasia of spermatogenic series and Leydig cells. In conclusion, unilateral orchidectomy had compensatory effects on the weight and volume of remaining testis. Adverse effects on sperm motility and viability can affect the fertility of the animal.

  20. Influence of Aluminium Chloride on Antioxidant System in the Testis and Epididymis of Rats

    Directory of Open Access Journals (Sweden)

    Arumugam Kalaiselvi

    2014-03-01

    Full Text Available Background: In recent years, the use of chemicals in agriculture, industry, and public health has become so common that the environment is continuously contaminated by the toxic substance-like metals. Aluminum released due to anthropogenic activities such as mining and industrial uses. Aluminium has several industrial uses. The present study was designed to investigate the effect of aluminium chloride (AlCl3 on enzymatic and non-enzymatic antioxidants in the testis and epididymis of rats. Methods: Adult male rats were administered with aluminium chloride at two different doses, 50 mg and 100 mg/kg body weight, orally, daily for 45 days. At the end of the experimental period, the animals were sacrificed and their testis and the epididymis were removed. Antioxidant enzymes like catalase (CAT, superoxide dismutase (SOD, glutathione peroxidase (GPx, glutathione reductase (GR, and glutathione-s-transferase (GST were assayed. Lipid peroxidation (LPO, vitamin C, and vitamin E levels were also determined. Results: Aluminium chloride administration had no effect on the bodyweight of the animals but the weight of the testis and epididymis was decreased. Almost all the antioxidant enzymes studied markedly diminished in the testis and epididymis of aluminium chloride treated animals. The non-enzymatic antioxidants, vitamin C and vitamin E, also declined. Lipid peroxidation, on the other hand, significantly increased. The influence was found to be more in 100 mg treated rats when compared to 50 mg treated rats. Conclusions: The present study suggests the reproductive toxicity of aluminium by inducing the oxidative stress in the testis and epididymis and possible interference in sperm production and further maturational processes.

  1. The gut microbiota and developmental programming of the testis in mice.

    Directory of Open Access Journals (Sweden)

    Maha Al-Asmakh

    Full Text Available Nutrients and environmental chemicals, including endocrine disruptors, have been incriminated in the current increase in male reproductive dysfunction, but the underlying mechanisms remain unknown. The gastrointestinal tract represents the largest surface area exposed to our environment and thereby plays a key role in connection with exposure of internal organs to exogenous factors. In this context the gut microbiome (all bacteria and their metabolites have been shown to be important contributors to body physiology including metabolism, cognitive functions and immunity. Pivotal to male reproduction is a proper development of the testis, including the formation of the blood-testis barrier (BTB that encapsulates and protects germ cells from stress induced environmental cues, e.g. pathogenic organisms and xenobiotics. Here we used specific pathogen free (SPF mice and germ-free (GF mice to explore whether gut microbiota and/or their metabolites can influence testis development and regulation of BTB. Lumen formation in the seminiferous tubules, which coincides with the development of the BTB was delayed in the testes of GF mice at 16 days postpartum. In addition, perfusion experiments (Evans blue demonstrated increased BTB permeability in these same mice. Reduced expressions of occludin, ZO-2 and E-cadherin in GF testis suggested that the microbiota modulated BTB permeability by regulation of cell-cell adhesion. Interestingly, exposure of GF mice to Clostridium Tyrobutyricum (CBUT, which secrete high levels of butyrate, restored the integrity of the BTB and normalized the levels of cell adhesion proteins. Moreover, the GF mice exhibited lower serum levels of gonadotropins (LH and FSH than the SPF group. In addition, the intratesticular content of testosterone was lower in GF compared to SPF or CBUT animals. Thus, the gut microbiome can modulate the permeability of the BTB and might play a role in the regulation of endocrine functions of the testis.

  2. Effect of varicocelectomy on testis volume and semen parameters in adolescents: a meta-analysis

    Directory of Open Access Journals (Sweden)

    Tie Zhou

    2015-01-01

    Full Text Available Varicocele repair in adolescent remains controversial. Our aim is to identify and combine clinical trials results published thus far to ascertain the efficacy of varicocelectomy in improving testis volume and semen parameters compared with nontreatment control. A literature search was performed using Medline, Embase and Web of Science, which included results obtained from meta-analysis, randomized and nonrandomized controlled studies. The study population was adolescents with clinically palpable varicocele with or without the testicular asymmetry or abnormal semen parameters. Cases were allocated to treatment and observation groups, and testis volume or semen parameters were adopted as outcome measures. As a result, seven randomized controlled trials (RCTs and nonrandomized controlled trials studying bilateral testis volume or semen parameters in both treatment and observation groups were identified. Using a random effect model, mean difference of testis volume between the treatment group and the observation group was 2.9 ml (95% confidence interval [CI]: 0.6, 5.2; P< 0.05 for the varicocele side and 1.5 ml (95% CI: 0.3, 2.7; P< 0.05 for the healthy side. The random effect model analysis demonstrated that the mean difference of semen concentration, total semen motility, and normal morphology between the two groups was 13.7 × 10 6 ml−1 (95% CI: −1.4, 28.8; P = 0.075, 2.5% (95% CI: −3.6, 8.6; P= 0.424, and 2.9% (95% CI: −3.0, 8.7; P= 0.336 respectively. In conclusion, although varicocelectomy significantly improved bilateral testis volume in adolescents with varicocele compared with observation cases, semen parameters did not have any statistically significant difference between two groups. Well-planned, properly conducted RCTs are needed in order to confirm the above-mentioned conclusion further and to explore whether varicocele repair in adolescents could improve subsequently spontaneous pregnancy rates.

  3. CRISPR/Cas9 Promotes Functional Study of Testis Specific X-Linked Gene In Vivo.

    Directory of Open Access Journals (Sweden)

    Minyan Li

    Full Text Available Mammalian spermatogenesis is a highly regulated multistage process of sperm generation. It is hard to uncover the real function of a testis specific gene in vitro since the in vitro model is not yet mature. With the development of the CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated 9 system, we can now rapidly generate knockout mouse models of testis specific genes to study the process of spermatogenesis in vivo. SYCP3-like X-linked 2 (SLX2 is a germ cell specific component, which contains a Cor1 domain and belongs to the XLR (X-linked, lymphocyte regulated family. Previous studies suggested that SLX2 might play an important role in mouse spermatogenesis based on its subcellular localization and interacting proteins. However, the function of SLX2 in vivo is still elusive. Here, to investigate the functions of SLX2 in spermatogenesis, we disrupted the Slx2 gene by using the CRISPR/Cas9 system. Since Slx2 is a testis specific X-linked gene, we obtained knockout male mice in the first generation and accelerated the study process. Compared with wild-type mice, Slx2 knockout mice have normal testis and epididymis. Histological observation of testes sections showed that Slx2 knockout affected none of the three main stages of spermatogenesis: mitosis, meiosis and spermiogenesis. In addition, we further confirmed that disruption of Slx2 did not affect the number of spermatogonial stem cells, meiosis progression or XY body formation by immunofluorescence analysis. As spermatogenesis was normal in Slx2 knockout mice, these mice were fertile. Taken together, we showed that Slx2 itself is not an essential gene for mouse spermatogenesis and CRISPR/Cas9 technique could speed up the functional study of testis specific X-linked gene in vivo.

  4. The germ cell nuclear proteins hnRNP G-T and RBMY activate a testis-specific exon.

    OpenAIRE

    Yilei Liu; Cyril F Bourgeois; Shaochen Pang; Marek Kudla; Natacha Dreumont; Liliane Kister; Yong-Hua Sun; James Stevenin; David J Elliott

    2009-01-01

    The human testis has almost as high a frequency of alternative splicing events as brain. While not as extensively studied as brain, a few candidate testis-specific splicing regulator proteins have been identified, including the nuclear RNA binding proteins RBMY and hnRNP G-T, which are germ cell-specific versions of the somatically expressed hnRNP G protein and are highly conserved in mammals. The splicing activator protein Tra2beta is also highly expressed in the testis and physically intera...

  5. Sperm protein 17: clinical relevance of a cancer/testis antigen, from contraception to cancer immunotherapy, and beyond.

    Science.gov (United States)

    Chiriva-Internati, Maurizio

    2011-01-01

    Sperm protein 17 belongs to the cancer/testis antigen family and was found to play a key role in the cell fusion process between the mammalian spermatozoa and egg. Sperm protein 17-specific autoantibodies in vasectomized males suggest its high expression in the testis. Sperm protein 17 expression levels are low or absent in normal tissues, other than the testis. Sperm protein 17 is expressed by tumors, including ovarian cancer, indicating that it is an ideal target for cancer immunotherapy, and plays a role in tumorigenesis and drug resistance. This review recapitulates the milestones of sperm protein 17 research and highlights its potential use in translational medicine.

  6. The Effect of Orally Administered L-carnitine on Testis Tissue Sperm Parameters and Daily Sperm Production in Adult Mice

    OpenAIRE

    Zohre Zare; Hossein Eimani; Moslem Mohammadi; Mahmood Mofid; Hossein Dashtnavard

    2010-01-01

    Introduction: The purpose of this study was to evaluate body and testis weight, testis tissue,counts, motility, viability, morphology, and chromatin quality of epididymal sperm, aswell as the testicular spermatid number (TSN) per gram of testis, and daily sperm production(DSP) in L-carnitine treated mice.Materials and Methods: In the present study, adult male NMRI mice (mean age of 4weeks) were administered L-carnitine by gavage for two weeks. The experimental groupsreceived 1mg L-carnitine/1...

  7. Investigation into the Effects of VHF and UHF Band Radiation on Hewlett-Packard (HP) Cesium Beam Frequency Standards

    National Research Council Canada - National Science Library

    Dickens, Andrew

    1994-01-01

    This paper documents an investigation into reports which have indicated that exposure to VHF and UHF band radiation has adverse effects on the frequency stability of HP cesium beam frequency standards...

  8. DEVELOPMENT OF AN ALGORITHM TO ESTIMATE EYE LENS DOSE IN TERMS OF OPERATIONAL QUANTITY Hp(3) USING HEAD TLD BADGE.

    Science.gov (United States)

    Madhumita, B; Sneha, C; Dipali, V; Pradhan, S M; Bakshi, A K; Datta, D; Tripathi, S M; Singh, S K

    2018-03-01

    In view of the recommendations of International Commission on Radiological Protection for reduction of the occupational annual dose limit for eye lens from 150 mSv to 20 mSv/y, questions have been raised on the adequacy of monitoring for the quantities Hp(10) and Hp(0.07). As an immediate requirement, in the present situation, where there is no exclusive eye lens dosemeter in India, the existing chest TLD badge was modified to be used as head badge (head dosemeter) by including a strap to enable wearing on the forehead. In order to estimate the eye lens dose in terms of the operational quantity Hp(3), the prevalent algorithm of chest badge was also modified. The modified algorithm was applied to estimate Hp(3) for dosemeters irradiated to various beta and photon radiations including mixtures. The Q values (estimated/delivered dose equivalent) were found to be within ±20% for most of the photon beams.

  9. Type testing of a head band dosemeter for measuring eye lens dose in terms of H(P)(3).

    Science.gov (United States)

    Gilvin, P J; Baker, S T; Gibbens, N J; Roberts, G H; Tanner, R J; Eakins, J S; Hager, L G; Daniels, T J

    2013-12-01

    A new head band dosemeter, for the measurement of eye lens dose in terms of Hp(3), has been type tested by Public Health England's Centre for Radiation, Environmental and Chemical Hazards [formerly part of the UK Health Protection Agency (HPA)]. The type tests were based on the International Standard ISO 12794, drawing also upon earlier work at HPA. The results show that, unlike many existing dosemeters, the new head band dosemeter correctly measures Hp(3) for beta radiations as well as photons.

  10. Biosynthesis of platform chemical 3-hydroxypropionic acid (3-HP) directly from CO2 in cyanobacterium Synechocystis sp. PCC 6803.

    Science.gov (United States)

    Wang, Yunpeng; Sun, Tao; Gao, Xingyan; Shi, Mengliang; Wu, Lina; Chen, Lei; Zhang, Weiwen

    2016-03-01

    3-hydroxypropionic acid (3-HP) is an important platform chemical with a wide range of applications. So far large-scale production of 3-HP has been mainly through petroleum-based chemical processes, whose sustainability and environmental issues have attracted widespread attention. With the ability to fix CO2 directly, cyanobacteria have been engineered as an autotrophic microbial cell factory to produce fuels and chemicals. In this study, we constructed the biosynthetic pathway of 3-HP in cyanobacterium Synechocystis sp. PCC 6803, and then optimized the system through the following approaches: i) increasing expression of malonyl-CoA reductase (MCR) gene using different promoters and cultivation conditions; ii) enhancing supply of the precursor malonyl-CoA by overexpressing acetyl-CoA carboxylase and biotinilase; iii) improving NADPH supply by overexpressing the NAD(P) transhydrogenase gene; iv) directing more carbon flux into 3-HP by inactivating the competing pathways of PHA and acetate biosynthesis. Together, the efforts led to a production of 837.18 mg L(-1) (348.8 mg/g dry cell weight) 3-HP directly from CO2 in Synechocystis after 6 days cultivation, demonstrating the feasibility photosynthetic production of 3-HP directly from sunlight and CO2 in cyanobacteria. In addition, the results showed that overexpression of the ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) gene from Anabaena sp. PCC 7120 and Synechococcus sp. PCC 7942 led to no increase of 3-HP production, suggesting CO2 fixation may not be a rate-limiting step for 3-HP biosynthesis in Synechocystis. Copyright © 2015 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.

  11. Secondary Haemophilus parasuis infection enhances highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) infection-mediated inflammatory responses.

    Science.gov (United States)

    Li, Jiangnan; Wang, Shengnan; Li, Changyao; Wang, Chunlai; Liu, Yonggang; Wang, Gang; He, Xijun; Hu, Liang; Liu, Yuanyuan; Cui, Mengmeng; Bi, Caihong; Shao, Zengyu; Wang, Xiaojie; Xiong, Tao; Cai, Xuehui; Huang, Li; Weng, Changjiang

    2017-05-01

    Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) infection often predisposes pigs to secondary bacterial infection, which induces robust inflammatory responses. However, whether the secondary bacterial infection synergizes HP-PRRSV infection and enhances inflammatory responses is not fully understood. Here, we characterized HP-PRRSV infection-mediated secondary bacterial infection and robust inflammatory responses. HP-PRRSV infection induced higher levels of cytokines (IL-1β, IL-18, IL-6 and TNF-α) in the sera in piglets and bacterial loads of 11 bacterial species in the lung were increased after HP-PRRSV infection, including Mycoplasma hyorhinis, Haemophilus parasuis and Escherichia coli. Concurrent infection with HP-PRRSV and H. parasuis model showed that inflammatory cytokines expression and secretion in porcine alveolar macrophages (PAMs) were increased in comparison with PAMs infected with HP-PRRSV or H. parasuis alone. Additionally, we found that H. parasuis RNA plays an important role in the robust inflammatory response enhancement in HP-PRRSV-infected PAMs. Taken together, our findings suggest that bacterial RNA transfection enhanced HP-PRRSV-mediated inflammatory responses in HP-PRRSV and H. parasuis (HPS) concurrent infection, which provides an important clue for comprehensive understanding of HP-PRRSV and bacterial coinfection-mediated pathology. Copyright © 2017 Elsevier B.V. All rights reserved.

  12. Hinge and chromoshadow of HP1α participate in recognition of K9 methylated histone H3 in nucleosomes.

    Science.gov (United States)

    Mishima, Yuichi; Watanabe, Makoto; Kawakami, Toru; Jayasinghe, Chanika D; Otani, Junji; Kikugawa, Yusuke; Shirakawa, Masahiro; Kimura, Hiroshi; Nishimura, Osamu; Aimoto, Saburo; Tajima, Shoji; Suetake, Isao

    2013-01-09

    The majority of the genome in eukaryotes is packaged into transcriptionally inactive chromatin. Heterochromatin protein 1 (HP1) is a major player in the establishment and maintenance of heterochromatin. HP1 specifically recognizes a methylated lysine residue at position 9 in histone H3 through its N-terminal chromo domain (CD). To elucidate the binding properties of HP1α to nucleosomes in vitro, we reconstituted nucleosomes containing histone H3 trimethylated at lysine 9. HP1α exhibited high-affinity binding to nucleosomes containing methylated histone H3 in a nucleosome core-number-dependent manner. The hinge region (HR) connecting the CD and C-terminal chromoshadow domain (CSD), and the CSD contributed to the selective binding of HP1α to histone H3 with trimethylated lysine 9 through weak DNA binding and by suppressing the DNA binding, respectively. We propose that not only the specific recognition of lysine 9 methylation of histone H3 by the CD but also the HR and the CSD cooperatively contribute to the selective binding of HP1α to histone H3 lysine 9 methylated nucleosomes. Copyright © 2012 Elsevier Ltd. All rights reserved.

  13. HP-NAP inhibits the growth of bladder cancer in mice by activating a cytotoxic Th1 response.

    Science.gov (United States)

    Codolo, Gaia; Fassan, Matteo; Munari, Fabio; Volpe, Andrea; Bassi, Piefrancesco; Rugge, Massimo; Pagano, Francesco; D'Elios, Mario Milco; de Bernard, Marina

    2012-01-01

    Intravesical Bacillus Calmette-Guérin (BCG) is the gold standard treatment for intermediate and high-risk non-muscle-invasive bladder cancer. BCG therapy is the most successful example of immunotherapy in cancer. Unfortunately, the treatment-related side effects are still relevant. Furthermore, non-responder patients are candidate to radical cystectomy in the absence of valuable alternative options. These aspects have prompted the search for newer biological response modifiers (BRM) with a better benefit/side effects ratio. The toll-like receptor (TLR) 2 ligand, Helicobacter pylori protein HP-NAP, has been shown to deserve a potential role as BRM. HP-NAP is capable of driving the differentiation of T helper (Th) 1 cells, both in vitro and in vivo, because of its ability to create an IL-12-enriched milieu. Herein, we report that local administration of HP-NAP decreases tumour growth by triggering tumour necrosis in a mouse model of bladder cancer implant. The effect is accompanied by a significant accumulation of both CD4+ and CD8+ IFN-γ-secreting cells, within tumour and regional lymph nodes. Noteworthy, HP-NAP-treated tumours show also a reduced vascularization due to the anti-angiogenic activity of IFN-γ induced by HP-NAP. Our findings strongly indicate that HP-NAP might become a novel therapeutic "bullet" for the cure of bladder tumours.

  14. A Comparison between the hp-version of Finite Element Method with EIDORS for Electrical Impedance Tomography.

    Science.gov (United States)

    Saeedizadeh, N; Kermani, S; Rabbani, H

    2011-07-01

    In this study, a hp-version of Finite Element Method (FEM) was applied for forward modeling in image reconstruction of Electrical Impedance Tomography (EIT). The EIT forward solver is normally based on the conventional Finite Element Method (h-FEM). In h-FEM, the polynomial order (p) of the element shape functions is constant and the element size (h) is decreasing. To have an accurate simulation with the h-FEM, a mesh with large number of nodes and elements is usually needed. In order to overcome this problem, the high order finite element method (p-FEM) was proposed. In the p-version, the polynomial order is increasing and the mesh size is constant. Combining the advantages of two previously mentioned methods, the element size (h) was decreased and the polynomial order (p) was increased, simultaneously, which is called the hp-version of Finite Element Method (hp-FEM). The hp-FEM needs a smaller number of nodes and consequently, less computational time and less memory to achieve the same or even better accuracy than h-FEM. The SNR value is 42db for hp-FEM and is 9db for h-FEM. The numerical results are presented and verified that the performance of the hp-version is better than of the h-version in image reconstruction of EIT.

  15. Serum levels of acute phase proteins: SAA, Hp and progesterone (P4) in mares with early embryonic death.

    Science.gov (United States)

    Krakowski, L; Krawczyk, C H; Kostro, K; Stefaniak, T; Novotny, F; Obara, J

    2011-08-01

    The study involved 46 healthy purebred Arabian mares exhibiting regular oestrous cycles that underwent artificial insemination (AI). Pregnancy was detected ultrasonographically (US) in 40 mares. In 15 mares in foal, early embryonic death (EED) was observed during the pregnancy days 14-21. Blood for determinations of serum acute phase proteins (SAA and Hp) and progesterone (P4) was sampled 12-24 h before ovulation and the first insemination, at 12, 24, 72, 96 h and on day 7, 10, 14, 21, 35 and 55 after ovulation. The results revealed that in 25 mares without EED, the serum levels of P4, SAA and Hp were within physiological limits; in 15 mares with EED, the levels of SAA and Hp were significantly increased. In seven mares with EED, high levels of SAA and Hp were already found before ovulation and at 12, 24, 72, 96 h as well as on day 7 and 10 post-ovulation, whereas the level of P4 was normal for early pregnancy. In the remaining eight mares with EED, increased levels of SAA and Hp were found at 72 h after ovulation and maintained until day 55. In this group, the level of P4 decreased since 96 h after ovulation. Determinations of SAA, Hp and P4 in mares in early pregnancy (EP) are useful for monitoring normal development of pregnancy and for diagnosis of subclinical genital inflammations, which may lead to EED. © 2011 Blackwell Verlag GmbH.

  16. DhHP-6 extends lifespan of Caenorhabditis elegans by enhancing nuclear translocation and transcriptional activity of DAF-16.

    Science.gov (United States)

    Huang, Lei; Li, Pengfei; Wang, Guan; Guan, Shuwen; Sun, Xiaoli; Wang, Liping

    2013-04-01

    Earlier studies have demonstrated that Deuterohaemin-AlaHisThrValGluLys (DhHP-6), a novel porphyrin-peptide, increases lifespan and enhances stress resistance of Caenorhabditis elegans. To explore the possible mechanisms, in this study we investigated the roles of SIR-2.1 and DAF-16 in DhHP-6's function using wild-type and various other mutant strains of C. elegans. DhHP-6's effect was dependent upon DAF-16, and it did not extend the lifespan of the loss-of-function daf-16 mutant strain (daf-16(mu86) I). DhHP-6 enhanced DAF-16 translocation from cytoplasm to nuclei; and it increased DAF-16's transcriptional activity, likely by activating the SIR-2.1/DAF-16 complex. DhHP-6's effect was also dependent upon SIR-2.1, and it did not increase the lifespan of the worms with SIR-2.1 deacetylase activity inhibited by niacin amide (SIR-2.1 inhibitor) and SIR-2.1 RNA interference (RNAi). Niacin amide and RNAi increased DAF-16's nuclear localization; but they decreased DAF-16's transcriptional activity, likely by preventing the formation of the SIR-2.1/DAF-16 complex. These results suggest that DhHP-6 extends the lifespan of C. elegans via SIR 2.1 and DAF-16, and they provide new insights into the molecular mechanisms of aging.

  17. Use of the processes vaginalis: A new technique for reinforcing the neourethra in hypospadias associated with undescended testis

    Directory of Open Access Journals (Sweden)

    Himanshu Acharya

    2009-01-01

    Conclusions: In patients of undescended testis with hypospadias, simultaneous repair with the processes vaginalis flap is an ideal technique with good results. Processes vaginalis is good vascular cover for neourethra.

  18. Expression and action of transforming growth factor beta (TGFbeta1, TGFbeta2, and TGFbeta3) during embryonic rat testis development.

    Science.gov (United States)

    Cupp, A S; Kim, G; Skinner, M K

    1999-06-01

    The objective of the current study was to determine the role of transforming growth factor beta (TGFbeta) during seminiferous cord formation and embryonic testis development. The expression pattern of mRNA for TGFbeta isoforms was evaluated during testis development through a quantitative reverse transcription-polymerase chain reaction (QRT-PCR) procedure. Expression of mRNA for TGFbeta1 was highest at postnatal day 0 (P0) and P10. In contrast, TGFbeta2 was high at embryonic day 15 (E15), declined at E16, and showed a transient increase at P0 through P3 of testis development. Interestingly, expression of mRNA for TGFbeta3 was high during embryonic development and then declined after P3. Immunohistochemical localization of TGFbeta1 and TGFbeta2 demonstrated expression in Sertoli cells at E14 and in the seminiferous cords at P0. Selective interstitial cells expressed high concentrations of TGFbeta1 and TGFbeta2 in P0 testis. TGFbeta3 was expressed in selective cells at the junction of the E14 testis and mesonephros. The cells expressing TGFbeta3 in the testis appeared to be preperitubular cells that resided around the seminiferous cords. TGFbeta3 was localized to gonocytes in P0 testis. TGFbeta1 was found to have no influence on seminiferous cord formation in embryonic organ cultures of E13 testis. In contrast, growth of both E13 and E14 embryonic organ cultures was inhibited by TGFbeta1 and resulted in reduced testis size (40% of controls) with fewer cords present. A P0 testis cell culture and thymidine incorporation assay were used to directly examine the effects of recombinant TGFbeta1. TGFbeta1 alone had no influence on thymidine incorporation in P0 testis cell cultures when compared to controls. Interestingly, TGFbeta1 inhibited epidermal growth factor (EGF), and 10% calf serum stimulated P0 testis cell growth but not FSH-stimulated growth. Therefore, TGFbeta1 appears to inhibit testis growth in both the embryonic and early postnatal periods. The hormonal

  19. Lack of ADAM2, CALR3 and SAGE1 Cancer/Testis Antigen Expression in Lung and Breast Cancer

    DEFF Research Database (Denmark)

    Maheswaran, Emeaga; Pedersen, Christina B; Ditzel, Henrik J

    2015-01-01

    inhibitors has been proposed as an attractive strategy to increase the expression of cancer/testis antigens in tumors before immunotargeting; however, neither ADAM2, CALR3 nor SAGE1 could be significantly induced in lung and breast cancer cell lines using this strategy. Our results suggest that ADAM2, CALR3...... and antigenic properties, but the expression patterns of most of the more than 200 identified cancer/testis antigens in various cancers remain largely uncharacterized. In this study, we investigated the expression of the cancer/testis antigens ADAM2, CALR3 and SAGE1 in lung and breast cancer, the two most...... frequent human cancers, with the purpose of providing novel therapeutic targets for these diseases. We used a set of previously uncharacterized antibodies against the cancer/testis antigens ADAM2, CALR3 and SAGE1 to investigate their expression in a large panel of normal tissues as well as breast and lung...

  20. An HP Adaptive Discontinuous Galerkin Method for Hyperbolic Conservation Laws. Ph.D. Thesis

    Science.gov (United States)

    Bey, Kim S.

    1994-01-01

    This dissertation addresses various issues for model classes of hyperbolic conservation laws. The basic approach developed in this work employs a new family of adaptive, hp-version, finite element methods based on a special discontinuous Galerkin formulation for hyperbolic problems. The discontinuous Galerkin formulation admits high-order local approximations on domains of quite general geometry, while providing a natural framework for finite element approximations and for theoretical developments. The use of hp-versions of the finite element method makes possible exponentially convergent schemes with very high accuracies in certain cases; the use of adaptive hp-schemes allows h-refinement in regions of low regularity and p-enrichment to deliver high accuracy, while keeping problem sizes manageable and dramatically smaller than many conventional approaches. The use of discontinuous Galerkin methods is uncommon in applications, but the methods rest on a reasonable mathematical basis for low-order cases and has local approximation features that can be exploited to produce very efficient schemes, especially in a parallel, multiprocessor environment. The place of this work is to first and primarily focus on a model class of linear hyperbolic conservation laws for which concrete mathematical results, methodologies, error estimates, convergence criteria, and parallel adaptive strategies can be developed, and to then briefly explore some extensions to more general cases. Next, we provide preliminaries to the study and a review of some aspects of the theory of hyperbolic conservation laws. We also provide a review of relevant literature on this subject and on the numerical analysis of these types of problems.

  1. Transcriptome analysis of spermatogenically regressed, recrudescent and active phase testis of seasonally breeding wall lizards Hemidactylus flaviviridis.

    Directory of Open Access Journals (Sweden)

    Mukesh Gautam

    Full Text Available Reptiles are phylogenically important group of organisms as mammals have evolved from them. Wall lizard testis exhibits clearly distinct morphology during various phases of a reproductive cycle making them an interesting model to study regulation of spermatogenesis. Studies on reptile spermatogenesis are negligible hence this study will prove to be an important resource.Histological analyses show complete regression of seminiferous tubules during regressed phase with retracted Sertoli cells and spermatognia. In the recrudescent phase, regressed testis regain cellular activity showing presence of normal Sertoli cells and developing germ cells. In the active phase, testis reaches up to its maximum size with enlarged seminiferous tubules and presence of sperm in seminiferous lumen. Total RNA extracted from whole testis of regressed, recrudescent and active phase of wall lizard was hybridized on Mouse Whole Genome 8×60 K format gene chip. Microarray data from regressed phase was deemed as control group. Microarray data were validated by assessing the expression of some selected genes using Quantitative Real-Time PCR. The genes prominently expressed in recrudescent and active phase testis are cytoskeleton organization GO 0005856, cell growth GO 0045927, GTpase regulator activity GO: 0030695, transcription GO: 0006352, apoptosis GO: 0006915 and many other biological processes. The genes showing higher expression in regressed phase belonged to functional categories such as negative regulation of macromolecule metabolic process GO: 0010605, negative regulation of gene expression GO: 0010629 and maintenance of stem cell niche GO: 0045165.This is the first exploratory study profiling transcriptome of three drastically different conditions of any reptilian testis. The genes expressed in the testis during regressed, recrudescent and active phase of reproductive cycle are in concordance with the testis morphology during these phases. This study will pave

  2. A Multiplex Cancer/Testis Antigen-Based Biomarker Panel to Predict Aggressive Phenotype of Prostate Cancer

    Science.gov (United States)

    2015-10-01

    correlated with no protein translation. 15. SUBJECT TERMS Prostate cancer; metastatic prostate cancer; cancer/testis antigens (CTA); biomarker ; gene...Antigen retrieval was performed under heat and adequate pH. After that, steps for endogenous peroxide activity and unspecific protein blocking were...AWARD NUMBER: W81XWH-12-1-0535 TITLE: A Multiplex Cancer/Testis Antigen-Based Biomarker Panel to Predict Aggressive Phenotype of Prostate

  3. ADAPTATION OF THE HP LIFE PROGRAM FOR PROMOTION OF THE ENTREPRENEURSHIP AMONG YOUNG PEOPLE

    Directory of Open Access Journals (Sweden)

    Ivan Mihajlović

    2012-11-01

    Full Text Available This text is dealing with attempt of Management department, of Technical faculty in Bor, todevelop and further sustain the „entrepreneurial spirit“among young people. The subject of the paperis the HP LIFE program, in which Technical faculty in Bor is the only partner institution from Serbia.Besides up-to-date IT equipment obtained by Hewlett Packard in previous phases of this project,during 2012 this project was financially sustained by the Serbian institution „Centre for promotionof science“. This support will increase the scope of project activities during this year, including newtarget groups.

  4. Period Determination for 5049 Sherlock, 16852 Nuredduna and (16943) 1998 HP42

    Science.gov (United States)

    Marchini, Alessandro; Papini, Riccardo; Salvaggio, Fabio

    2018-04-01

    Photometric observations of three main-belt asteroids were conducted from the Astronomical Observatory of the University of Siena (Italy) in order to determine their synodic rotation periods. For 5049 Sherlock we found a period of 5.492 ± 0.001 h with an amplitude of 0.79 mag, for 16852 Nuredduna a period of 6.299 ± 0.002 h with an amplitude of 0.30 mag and for (16943) 1998 HP42 a period of 16.764 ± 0.006 h with an amplitude of 0.67 mag.

  5. pHP-Tethered N-Acyl Carbamate: A Photocage for Nicotinamide.

    Science.gov (United States)

    Salahi, Farbod; Purohit, Vatsal; Ferraudi, Guillermo; Stauffacher, Cynthia; Wiest, Olaf; Helquist, Paul

    2018-04-13

    The synthesis of a new photocaged nicotinamide having an N-acyl carbamate linker and a p-hydroxyphenacyl (pHP) chromophore is described. The photophysical and photochemical studies showed an absorption maximum at λ = 330 nm and a quantum yield for release of 11% that are dependent upon both pH and solvent. While the acyl carbamate releases nicotinamide efficiently, a simpler amide linker was inert to photocleavage. This photocaged nicotinamide has significant advantages with respect to quantum yield, absorbance wavelength, rate of release, and solubility that make it the first practical example of a photocaged amide.

  6. Silent decision: HP1 protein escorts heterochromatic RNAs to their destiny

    Science.gov (United States)

    Ren, Jie; Martienssen, Robert A

    2012-01-01

    EMBO J advance online publication 06072012; doi:10.1016/j.molcel.2012.05.009 Heterochromatin is classically perceived to be refractory to transcription because of its compact structure. However, Keller et al (2012) now demonstrated that heterochromatic transcripts can accumulate even when heterochromatin is normally packaged. By tracking down the fate of these heterochromatic RNAs, they revealed a new post-transcriptional mechanism of silencing in heterochromatin that involves the dynamic turnover of HP1Swi6 between its free, chromatin-bound and RNA-bound forms. The latter form escorts heterochromatic RNA to degradation. PMID:22705945

  7. Mechanical behaviour of Astm A 297 grade Hp joints welded using different processes

    International Nuclear Information System (INIS)

    Emygdio, Paulo Roberto Oliveira; Zeemann, Annelise; Almeida, Luiz Henrique de

    1996-01-01

    The influence of different arc welding processes on mechanical behaviour was studied for cast heat resistant stainless steel welded joints, in the as welded conditions. ASTM A 297 grade HP with niobium and niobium/titanium additions were welded following three different welding procedures, using shielded metal arc welding gas tungsten arc welding and plasma arc welding, in six welded joints. The welded joint mechanical behaviour was evaluated by ambient temperature and 870 deg C tensile tests; and creep tests at 900 deg C and 50 MPa. Mechanical test results showed that the welding procedure qualification following welding codes is not suitable for high temperature service applications. (author)

  8. Application of a Long Term Asset Management Strategy for HP Feedwater Heaters

    International Nuclear Information System (INIS)

    Won, Se Youl; Yun, Eun Sub; Park, Young Sheop

    2008-01-01

    As the commercial operating year of nuclear power plants is increased, it becomes imperative to develop integrated cost-effective asset management and to improve plans for degraded Structures, Systems, and Components (SSCs) in terms of safety and economical consideration. A long-term asset management (LTAM) strategy can improve the condition of nuclear plants, maximize their value, and optimize their operational life by maintaining their safety. This paper presents an optimized LTAM plan for HP feedwater heaters at a specific nuclear power plant

  9. The Spectral/hp-Finite Element Method for Partial Differential Equations

    DEFF Research Database (Denmark)

    Engsig-Karup, Allan Peter

    2009-01-01

    dimensions. In the course the chosen programming environment is Matlab, however, this is by no means a necessary requirement. The mathematical level needed to grasp the details of this set of notes requires an elementary background in mathematical analysis and linear algebra. Each chapter is supplemented......This set of lecture notes provides an elementary introduction to both the classical Finite Element Method (FEM) and the extended Spectral/$hp$-Finite Element Method for solving Partial Differential Equations (PDEs). Many problems in science and engineering can be formulated mathematically...

  10. Microstructure of Magnesium alloy AZ91-HP cast in permanent and non-permanent moulds

    DEFF Research Database (Denmark)

    Tiedje, Niels; Sørensen, Rasmus Kirkegaard; Albachari, Sami

    2003-01-01

    Casting of different geometries were made with AZ91-HP in different moulding media, so that a range of cooling conditions were obtained. Cooling curves were measured and compared to the microstructures found in the castings. It was shown that segregation in the alloy is highly dependent on cooling...... rate. This leads to precipitation of varying amounts of intermetallic phases. The distribution of phases in the material is controlled both by interdendritic and intergranular segregation, leading to an inhomogeneous microstructure. It is shown that local solidification time, segregation and diffusion...

  11. Quantification of bitumen particles in aerosol and soil samples using HP-GPC

    DEFF Research Database (Denmark)

    Fauser, Patrik; Tjell, Jens Christian; Mosbæk, Hans

    2000-01-01

    A method for identifying and quantifying bitumen particles, generated from the wear of roadway asphalts, in aerosol and soil samples has been developed. Bitumen is found to be the only contributor to airborne particles containing organic molecules with molecular weights larger than 2000 g pr. mol....... These are separated and identified using High Performance Gel Permeation Chromatography (HP-GPC) with fluorescence detection. As an additional detection method Infra Red spectrometry (IR) is employed for selected samples. The methods have been used on aerosol, soil and other samples....

  12. Immunotherapy for adenoid cystic carcinoma of salivary glands: cancer/testis antigens and 5-aza-2'-deoxycytidine.

    Science.gov (United States)

    Liang, Suxia; Fan, Mingwen

    2009-11-01

    Adenoid cystic carcinoma of salivary glands is the epithelial tumor. There are amount of malignant occurrences of adenoid cystic carcinoma of salivary glands in the head and neck area. Cancer/testis antigens can be found in various malignant tumors, normal adult testis and occasionally placenta, but not in the other normal adult tissues. This characteristic makes Cancer/testis antigens as potential markers to be applied in immunotherapeutic strategies against cancer. It has been shown that in different tumors, the expression of certain Cancer/testis antigens is activated treated with 5-aza-CdR via the demethylation of their promoter CpG islands. It is logical that multiple Cancer/testis antigens may correlate with the clinicopathologic factors of adenoid cystic carcinoma of salivary glands and be the potential markers of prognosis treated with 5-aza-CdR. So the hypothesis will provide the new direction that we can use Cancer/testis antigens as candidate antigens for adenoid cystic carcinoma of salivary glands immunotherapy due to the high expression rate activated with 5-aza-CdR.

  13. Unilateral orchidectomy in donkey (Equus asinus): Evaluation of different surgical techniques, histological and morphological changes on remaining testis.

    Science.gov (United States)

    Mahmoud Ali Omar, Magda; Mohamed Ahmed Hassanein, Khaled; Khalifa Abdel-Razek, Abdel-Razek; Ali Yousef Hussein, Haroon

    2013-01-01

    Unilateral orchidectomy (UO) is required when further breeding potential is important. It is sometimes necessary to remove a single testis in a matured stallion for therapeutic reasons. In this study, twelve donkeys were used to evaluate three techniques of unilateral castration, histological and morphological changes on the remaining testis. Results of the study showed that each of the surgical techniques used had its advantages and disadvantages in comparison with the other two techniques. Therefore the selection among the three techniques depends on the surgeon preferences and the environment in which the unilateral orchidectomy is performed. The volume of the remaining testis recorded at the end of the study was significantly greater than that estimated at the start of the study (p < 0.05). The percentage of sperm motility obtained from the remaining testis was significantly decreased (p < 0.05). Histological examination of the testis in open surgery (group I) (where the scrotum was left opened) revealed severe hemorrhages, edema and fibrosis. The test is showed degenerative changes in the seminiferous tubules and interstitial orchitis. Histological examination of the testes removed using a closed technique, (in groups II and III) where the scrotum wound was sutured, revealed hyperplasia of spermatogenic series and Leydig cells. In conclusion, unilateral orchidectomy had compensatory effects on the weight and volume of remaining testis. Adverse effects on sperm motility and viability can affect the fertility of the animal.

  14. Impaired testicular function in patients with carcinoma-in-situ of the testis

    DEFF Research Database (Denmark)

    Petersen, P M; Giwercman, A; Hansen, S W

    1999-01-01

    for testicular cancer. Biopsy of the contralateral testis had showed CIS in a group of 24 patients and no evidence of CIS in the other group of 30 patients. Semen quality and serum levels of testosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) were compared in these two groups of men...... after orchidectomy but before further treatment for testicular cancer. RESULTS: Significantly higher LH levels (median, 8.1 IU/L v 4.8 IU/L; P ...PURPOSE: To elucidate the biologic association between germ cell neoplasia and testicular dysfunction, through investigation of Leydig cell function and semen quality in men with carcinoma-in-situ (CIS) of the testis. PATIENTS AND METHODS: We examined two groups of men, unilaterally orchidectomized...

  15. Persistent mullerian duct syndrome with teratoma in an ectopic testis: imaging features

    Energy Technology Data Exchange (ETDEWEB)

    Narlawar, R.S.; Patankar, T. [Dept. of Radiology, Seth G. S. Medical College and K. E. M. Hospital, Parel, Mumbai (India); Shah, J.R.; Parikh, V. [Dept. of CT and Ultrasound, Research Centre, Mumbai, Maharashtra (India)

    2001-06-01

    The persistent mullerian duct syndrome represents a rare form of male pseudohermaphroditism, secondary to mullerian inhibiting factor (MIF) deficiency. We describe imaging findings in a 30-year-old male (46 XY karyotype) with bilateral cryptorchidism and mullerian duct anomalies (presence of uterus and fallopian tubes). Grade-III teratoma with yolk sac tumour was detected in one of the undescended testis, lying in the pelvic cavity. The other testis was in the inguinal canal. The rest of the wolffian duct structures (e. g. prostate, seminal vesicles) were nearly normal. Very few reports of imaging findings of this entity have been published thus far, probably because of the rarity of entity, incidental detection of most of the cases at surgery and relatively asymptomatic clinical presentation. (orig.)

  16. Distribution of terminal sugar residues in the testis of the spotted ray Torpedo marmorata.

    Science.gov (United States)

    Liguoro, Annamaria; Prisco, Marina; Mennella, Clotilde; Ricchiari, Loredana; Angelini, Francesco; Andreuccetti, Piero

    2004-08-01

    Lectins represent a class of proteins/glycoproteins binding specifically to terminal sugar residues. The present investigation aims to identify lectin-binding sites in testis of Torpedo marmorata. Using a panel of lectins coupled with fluoresceine isothiocyanate, we demonstrated that germ and somatic cells present in Torpedo testis contain glycoconjugates, whose distribution at the level of the surface, the cytoplasm and the nucleus changes during germ cell differentiation. Moreover our observations demonstrate that the germ cells undergoing apoptosis (Prisco et al., 2003a: Mol Reprod Dev 64:341-348) overexpress a residual sugar recognised by WFA lectin that can be considered a specific marker for apoptotic germ cells. Finally, our results indicate that there is a progressive increase in glycosilation during spermatogenesis, especially at the level of the acrosome in the spermatocyte-spermatid step, and that Leydig cells are differently stained in relation to the spermatogenetic cycle. Copyright 2004 Wiley-Liss, Inc.

  17. Gilgamesh is required for the maintenance of germline stem cells in Drosophila testis.

    Science.gov (United States)

    Chen, Dongsheng; Zhu, Xiangxiang; Zhou, Lijuan; Wang, Jian; Tao, Xiaoqian; Wang, Shuang; Sun, Fuling; Kan, Xianzhao; Han, Zhengqi; Gu, Yuelin

    2017-07-18

    Emerging evidence supports that stem cells are regulated by both intrinsic and extrinsic mechanisms. However, factors that determine the fate of stem cells remain incompletely understood. The Drosophila testis provides an exclusive powerful model in searching for potential important regulatory factors and their underlying mechanisms for controlling the fate of germline stem cells (GSCs). In this study, we have found that Drosophila gilgamesh (gish), which encodes a homologue of human CK1-γ (casein kinase 1-gamma), is required intrinsically for GSC maintenance. Our genetic analyses indicate gish is not required for Dpp/Gbb signaling silencing of bam and is dispensable for Dpp/Gbb signaling-dependent Dad expression. Finally, we show that overexpression of gish fail to dramatically increase the number of GSCs. These findings demonstrate that gish controls the fate of GSCs in Drosophila testis by a novel Dpp/Gbb signaling-independent pathway.

  18. Expression and localization of nerve growth factor (NGF in the testis of alpaca (llama pacos

    Directory of Open Access Journals (Sweden)

    Haidong Wang

    2011-04-01

    Full Text Available During alpaca testis development and spermatogenesis, nerve growth factor (NGF may play an importantrole. The main aim of this study was to determine the expression and localization of NGF in the alpacatestis, and to discuss the important role of NGF in alpaca reproductive characteristics. Immunohistochemicalstaining technique and real-time PCR were used. The expression of NGF in the same cells one-month old(newborn alpacas 12-month, and 24-month old alpacas showed significant differences (p 0.05; NGF at different cell stages showed nosignificant differences (p > 0.05. It suggests that NGF may be involved in the regulation of spermatogenesis,which provides direct evidence for NGF action in the alpaca testis during postnatal development and spermatogenesis.

  19. Blood-testis barrier and spermatogenesis: lessons from genetically-modified mice

    Directory of Open Access Journals (Sweden)

    Xiao-Hua Jiang

    2014-08-01

    Full Text Available The blood-testis barrier (BTB is found between adjacent Sertoli cells in the testis where it creates a unique microenvironment for the development and maturation of meiotic and postmeiotic germ cells in seminiferous tubes. It is a compound proteinous structure, composed of several types of cell junctions including tight junctions (TJs, adhesion junctions and gap junctions (GJs. Some of the junctional proteins function as structural proteins of BTB and some have regulatory roles. The deletion or functional silencing of genes encoding these proteins may disrupt the BTB, which may cause immunological or other damages to meiotic and postmeiotic cells and ultimately lead to spermatogenic arrest and infertility. In this review, we will summarize the findings on the BTB structure and function from genetically-modified mouse models and discuss the future perspectives.

  20. Rat Testis Damage Caused by Lead Sulfide Nanoparticles After Oral Exposure.

    Science.gov (United States)

    Cao, Yanhua; Wang, Dong; Li, Qingzhao; Deng, Hongliang; Shen, Jian; Zheng, Guoying; Sun, Miao

    2016-03-01

    Lead sulfide nanoparticals (PbS NPs) is an important semiconductor material due to its unique physical and chemical properties, but its potential health hazard to reproductive system is not clear. In the current study, we systematically explored the reproductive toxicity of PbS NPs in rats by measuring the body weight and testicular coefficient, testing serum testosterone levels, and studying the sperm survival rate and sperm abnormality rate. Furthermore, in order to study the toxic mechanism we performed lead contents measurements in testis, and investigated the pathology in testis. Our results confirmed that PbS NPs showed high reproductive toxicity due to PbS NPs in rats' testicular tissue by the establishment of PbS NPs chronic exposure model.

  1. Recent adverse trends in semen quality and testis cancer incidence among Finnish men

    DEFF Research Database (Denmark)

    Jørgensen, N; Vierula, M; Jacobsen, R

    2011-01-01

    Impaired semen quality and testicular cancer may be linked through a testicular dysgenesis syndrome of foetal origin. The incidence of testis cancer has been shown to increase among Finnish men, whereas there is no recent publication describing temporal trends in semen quality. Therefore, we...... carried out a prospective semen quality study and a registry study of testis cancer incidence among Finnish men to explore recent trends. A total of 858 men were investigated in the semen quality study during 1998-2006. Median sperm concentrations were 67 (95% CI 57-80) million/mL, 60 (51-71) and 48 (39...... included in the registry study, which confirmed the increasing incidence of testicular cancer in recent cohorts. These simultaneous and rapidly occurring adverse trends suggest that the underlying causes are environmental and, as such, preventable. Our findings necessitate not only further surveillance...

  2. STEREOLOGICAL QUANTITATION OF LEYDIG AND SERTOLI CELLS IN THE TESTIS FROM YOUNG AND OLD MEN

    Directory of Open Access Journals (Sweden)

    Peter M Petersen

    2011-05-01

    Full Text Available One of the newer stereological methods, the optical fractionator, was applied to the study of the effects of ageing on the human testis. The estimated total number of Sertoli and Leydig cells per testis in men younger than 30 years were 430×106 (CV = SD/mean = 0.35 and 117×106 (CV = 0.53, respectively, while in men older than 50 years the estimated total Sertoli cell number was 266×106 (CV = 0.46 and the mean Leydig cell number 83×106 (CV = 0.53. The difference between the number of Sertoli cells in men younger than 30 years compared with men older than 50 years was close to statistical significance (p = 0.052 while no differences was found in total Leydig cell number (p = 0.22.

  3. Testis cancer: role of radiotherapy in 2003; Cancer du testicule: place de la radiotherapie en 2003

    Energy Technology Data Exchange (ETDEWEB)

    Clippe, S. [Centre de Lutte Contre le Cancer Leon-Berard, Dept. de Radiotherapie, 69 - Lyon (France); Flechon, A.; Droz, J.P. [Centre de Lutte Contre le Cancer Leon-Berard, Dept. de Cancerologie Medicale, 69 - Lyon (France)

    2003-11-01

    Germ-cell minors of the testis are rare tumors of the young adult. Half of them are seminoma. The majority of patients have disease limited to the testis. Radiotherapy still remains the standard treatment of these patients. Almost all patients are cured by orchidectomy and radiotherapy on the lomboaortic area extended to homolateral iliac area. The dose is 24 to 30 Gy in a standard fractionation. Different studies are ongoing to reduce the irradiation field (omission of the pelvic irradiation), to decrease irradiation dose (to 20 Gy). Other treatment options are strict surveillance and adjuvant carbo-platin based chemotherapy. None of these options are standard treatments. A strict attention must be directed on controlateral germ-cell tumors and second cancers. (author)

  4. Purification of β-acetylglucosaminase and β-galactosidase from ram testis

    Science.gov (United States)

    Caygill, J. C.; Roston, Christine P. J.; Jevons, F. R.

    1966-01-01

    1. The presence of β-galactosidase (EC 3.2.1.23) in an acetic acid extract of ram testis is reported. Some properties of the crude enzyme preparation were studied. 2. The purification of β-acetylglucosaminase (EC 3.2.1.30) and of β-galactosidase from the ram-testis extract by ammonium sulphate precipitation and chromatography on a CM-cellulose column is described. 3. The final purifications of the separated enzymes achieved were for the β-acetylglucosaminase 35 times and for the β-galactosidase 99 times. 4. The possibility of using DEAE-cellulose and Sephadex G-200 to purify the enzymes was investigated. PMID:5949569

  5. Late effects of ionizing radiation on testis; Effets tardifs des radiations ionisantes sur le testicules

    Energy Technology Data Exchange (ETDEWEB)

    Bardet, E. [Centre Regional de Lutte Contre le Cancer Rene-Gauducheau, 44 - Nantes (France)

    1997-12-01

    Most of the basic data regarding the effect of radiation on the testis are issued from animal studies. They demonstrate the extreme radiosensitivity on the germ cell lineage but little is known about the reversible or definitive aspects of these radiation induced effects. In man, the late non stochastic effects of radiation to the testicle are mainly related to persisting spermatogenesis disturbances or/and hormone related problems. Morphological, physiological, radiobiological specificities of the human testis along with numerous parameters depending on radiation conditions make it difficult to evaluate the late effects and radiation tolerance doses. Evaluation of such effects based on a common scale for therapeutic radiation schedules would improve the present understanding and possibility prevent the occurrence of these delayed effects, for the benefit of patients. (author)

  6. Metastasis of sigmoid colon cancer in cryptorchid testis: report of a case.

    Science.gov (United States)

    Rampa, Mario; Battaglia, Luigi; Caprotti, Andrea; Gazzano, Giacomo; Prestianni, Pierpaolo; Muscarà, Cecilia; Vannelli, Alberto

    2012-01-01

    Isolated testicular metastasis from colorectal cancer is considered an unusual event. In this case report we describe for the first time a metastasis from an adenocarcinoma of the sigmoid colon to a cryptorchid testis. The patient developed a painless testicular nodule three years after the diagnosis of primary sigmoid colon cancer. Recent reports have suggested that the incidence of genitourinary abnormalities in human males has increased over the past 50 years; in particular, cryptorchid testes increase the clinical risk factors for primary or metastatic testicular cancer. In conclusion, there should be awareness of the risk of metastasis of colorectal cancer to the testis in the workup of patients with testicular symptoms. Furthermore, patients with colorectal cancer and cryptorchidism should be managed with a single surgical intervention: when the primary colorectal tumor is removed, the cryptorchid testicle should also be removed to reduce the risk of late metastases.

  7. Biochemical characterization of glycyrrhizin as an effective inhibitor for hyaluronidases from bovine testis.

    Science.gov (United States)

    Furuya, T; Yamagata, S; Shimoyama, Y; Fujihara, M; Morishima, N; Ohtsuki, K

    1997-09-01

    The inhibitory effects of several anti-inflammatory agents, including glycyrrhizin (GL), on the activities of hyaluronidases (HAses) purified from bovine testes and Streptomyces were investigated in vitro. It was found that (i) GL inhibits the activity of HAse (p55) from bovine testes in a dose-dependent manner, but does not affect HAse from Streptomyces; (ii) GL was the most effective of the compounds tested on bovine testis HAse activity (50% inhibition with approx. 3 microM GL); and (iii) glycyrrhetinic acid (GA), a derivative (oGA) of GA and diglucuronic acid had no detectable effects on HAse activity at 9.0 microM. The GL-induced inhibition of HAse activity is uncompetitive for its substrates. Data are provided to support the contentions that (i) bovine testis HAse (p55) is a GL-binding protein; and (ii) GL acts as a potent inhibitor of HAse in vitro.

  8. D-type cyclins in adult human testis and testicular cancer

    DEFF Research Database (Denmark)

    Bartkova, J; Rajpert-de Meyts, E; Skakkebaek, N E

    1999-01-01

    D-type cyclins are proto-oncogenic components of the 'RB pathway', a G1/S regulatory mechanism centred around the retinoblastoma tumour suppressor (pRB) implicated in key cellular decisions that control cell proliferation, cell-cycle arrest, quiescence, and differentiation. This study focused...... on immunohistochemical and immunochemical analysis of human adult testis and 32 testicular tumours to examine the differential expression and abundance of cyclins D1, D2, and D3 in relation to cell type, proliferation, differentiation, and malignancy. In normal testis, the cell type-restricted expression patterns were...... dominated by high levels of cyclin D3 in quiescent Leydig cells and the lack of any D-type cyclin in the germ cells, the latter possibly representing the only example of normal mammalian cells proliferating in the absence of these cyclins. Most carcinoma-in-situ lesions appeared to gain expression of cyclin...

  9. HP1-mediated formation of alternative lengthening of telomeres-associated PML bodies requires HIRA but not ASF1a.

    Directory of Open Access Journals (Sweden)

    Wei-Qin Jiang

    Full Text Available Approximately 10% of cancers use recombination-mediated Alternative Lengthening of Telomeres (ALT instead of telomerase to prevent telomere shortening. A characteristic of cells that utilize ALT is the presence of ALT-associated PML nuclear bodies (APBs containing (TTAGGGn DNA, telomere binding proteins, DNA recombination proteins, and heterochromatin protein 1 (HP1. The function of APBs is unknown and it is possible that they are functionally heterogeneous. Most ALT cells lack functional p53, and restoration of the p53/p21 pathway in these cells results in growth arrest/senescence and a substantial increase in the number of large APBs that is dependent on two HP1 isoforms, HP1α and HP1γ. Here we investigated the mechanism of HP1-mediated APB formation, and found that histone chaperones, HIRA and ASF1a, are present in APBs following activation of the p53/p21 pathway in ALT cells. HIRA and ASF1a were also found to colocalize inside PML bodies in normal fibroblasts approaching senescence, providing evidence for the existence of a senescence-associated ASF1a/HIRA complex inside PML bodies, consistent with a role for these proteins in induction of senescence in both normal and ALT cells. Moreover, knockdown of HIRA but not ASF1a significantly reduced p53-mediated induction of large APBs, with a concomitant reduction of large HP1 foci. We conclude that HIRA, in addition to its physical and functional association with ASF1a, plays a unique, ASF1a-independent role, which is required for the localization of HP1 to PML bodies and thus for APB formation.

  10. Personal dose equivalent Hp(0.07) of medical personnel occupationally exposed to ionizing radiation during 2001-2011.

    Science.gov (United States)

    Papierz, Sylwia; Kacprzyk, Janusz; Kamiński, Zbigniew; Adamowicz, Małgorzata; Zmyślony, Marek

    2012-01-01

    The paper presents the results of the determinations of the personal dose equivalent Hp(0.07) received by medical employees of interventional radiology departments occupationally exposed to X and gamma rays in Poland in 2011, performed by the Nofer Institute of Occupational Medicine in Łódź. The results were compared with the data collected during the last decade. The dosimetric service provided for medical employees of interventional radiology departments occupationally exposed to ionizing radiation in terms of the personal dose equivalent Hp(0.07). In 2011, personal dosimetry Hp(0.07) determinations performed by the Nofer Institute of Occupational Medicine in Łódź covered 2017 workers employed in 159 medical laboratories. The determinations were performed using ring dosimeters equipped with thermoluminescence detectors according to the procedure accredited by the Polish Centre for Accreditation (document number AB 327). The determinations were carried out in one or two-month periods. Mean annual personal dose equivalent Hp(0.07) in 2011 was equal to 4.9 mSv (annual limit for Hp(0.07) is 500 mSv). The mean annual doses of Hp(0.07) varied from 7.6 mSv in 2001 to 5.6 mSv in 2010. In 2011, two cases of exceeding the annual dose limit of Hp(0.07) were reported. The results show that more than 95% of all examined annual doses did not exceed the level of 10 mSv. The comparison of the average annual doses and detailed distributions of the doses during the last few years suggests a stabilized level of occupational exposure and an acceptable level of radiological protection in interventional radiology departments monitored by NIOM in Łódź.

  11. Assessment of individual dose equivalents Hp(0.07) of medical staff occupationally exposed to ionizing radiation in 2012.

    Science.gov (United States)

    Papierz, Sylwia; Kamiński, Zbigniew; Adamowicz, Małgorzata; Zmyślony, Marek

    2014-01-01

    The paper presents the Nofer Institutes of Occupational Medicine in Łódź's results of the assessment of individual dose equivalents Hp(0.07) of medical staff exposed to X-rays in Poland in 2012. In addition, the collected data was analysed in terms of types of medical units performing medical procedures and the categorization of personnel. Dosimetric service was provided for medical staff of interventional radiology departments occupationally exposed to ionizing radiation in terms of individual dose equivalents Hp(0.07). In 2012, personal dosimetry Hp(0.07) determinations were performed by the Nofer Institute of Occupational Medicine in Łódź and covered 2044 employees from 174 health facilities. The determinations were performed using thermoluminescence dosimetry according to the procedure accredited by the Polish Centre for Accreditation (document number AB 327). The measurements were performed using ring-dosimeters in the periods of 1 or 2 months. Mean annual individual dose equivalent Hp(0.07) in 2012 was equal to 3.3 mSv (annual limit for Hp(0.07) is 500 mSv). The average value of annual individual dose equivalent Hp(0.07) decreased comparing to the previous year. In 2012, no single case of exceeding the annual limit for Hp(0.07) was reported. Data stored in the file indicates that more than 96% of all of the annual doses did not exceed the level of 10 mSv. The analysis of data on occupational exposure to ionizing radiation confirms a stable level of exposure and satisfactory radiological protection in interventional radiology facilities monitored by the Nofer Institute of Occupational Medicine in Łódź in Poland in 2012.

  12. HP1 recruits activity-dependent neuroprotective protein to H3K9me3 marked pericentromeric heterochromatin for silencing of major satellite repeats.

    Directory of Open Access Journals (Sweden)

    Kerstin Mosch

    Full Text Available H3 lysine 9 trimethylation (H3K9me3 is a histone posttranslational modification (PTM that has emerged as hallmark of pericentromeric heterochromatin. This constitutive chromatin domain is composed of repetitive DNA elements, whose transcription is differentially regulated. Mammalian cells contain three HP1 proteins, HP1α, HP1β and HP1γ These have been shown to bind to H3K9me3 and are thought to mediate the effects of this histone PTM. However, the mechanisms of HP1 chromatin regulation and the exact functional role at pericentromeric heterochromatin are still unclear. Here, we identify activity-dependent neuroprotective protein (ADNP as an H3K9me3 associated factor. We show that ADNP does not bind H3K9me3 directly, but that interaction is mediated by all three HP1 isoforms in vitro. However, in cells ADNP localization to areas of pericentromeric heterochromatin is only dependent on HP1α and HP1β. Besides a PGVLL sequence patch we uncovered an ARKS motif within the ADNP homeodomain involved in HP1 dependent H3K9me3 association and localization to pericentromeric heterochromatin. While knockdown of ADNP had no effect on HP1 distribution and heterochromatic histone and DNA modifications, we found ADNP silencing major satellite repeats. Our results identify a novel factor in the translation of H3K9me3 at pericentromeric heterochromatin that regulates transcription.

  13. Long-term testicular volume after orchiopexy at diagnosis of acquired undescended testis.

    Science.gov (United States)

    van der Plas, Evelyn M; Zijp, Gerda W; Froeling, Frank M J A; van der Voort-Doedens, Laszla M; Meij-de Vries, Annebeth; Goede, Joery; Hack, Wilfried W M

    2013-07-01

    We studied long-term outcomes of orchiopexy at diagnosis of acquired undescended testes using ultrasound to determine testicular volume. Patients who had undergone orchiopexy for acquired undescended testis at diagnosis were recruited to assess testicular volume. Testis volume was measured by ultrasound and compared with recently developed normative values for testicular size. For young adults (older than 18 years) volumes were grouped and compared to normative values reported in the literature. In all unilateral cases testicular volume was compared with its counterpart. A total of 155 patients 5.1 to 26.6 years old (181 acquired undescended testes) were included in the study. Mean ± SD followup was 6.6 ± 3.8 years (range 1.4 to 15.5). For all patients 18 years old or younger (125 patients, 143 testes) operated testis volume was 0.1 to 12.7 ml (mean ± SD 2.5 ± 2.9), which was significantly smaller than the normative values (50th percentile) for the same age (p <0.001). Mean ± SD testis volume in young adults (38 testes) was 8.1 ± 3.7 ml, compared to a mean volume of 13.4 ml reported in the literature (p <0.001). In unilateral cases the mean volume of the testes fixed by orchiopexy differed significantly from their counterparts (3.4 ± 3.3 ml vs 4.6 ± 4.6 ml, p <0.001). The long-term volumes at diagnosis of acquired undescended testes after orchiopexy were significantly less than the normative values at all ages. In unilateral cases the volumes were also significantly less compared to the contralateral testes. Copyright © 2013 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved.

  14. Gene expression profiling in liver and testis of rats to characterize the toxicity of triazole fungicides

    International Nuclear Information System (INIS)

    Tully, Douglas B.; Bao Wenjun; Goetz, Amber K.; Blystone, Chad R.; Ren, Hongzu; Schmid, Judith E.; Strader, Lillian F.; Wood, Carmen R.; Best, Deborah S.; Narotsky, Michael G.; Wolf, Douglas C.; Rockett, John C.; Dix, David J.

    2006-01-01

    Four triazole fungicides were studied using toxicogenomic techniques to identify potential mechanisms of action. Adult male Sprague-Dawley rats were dosed for 14 days by gavage with fluconazole, myclobutanil, propiconazole, or triadimefon. Following exposure, serum was collected for hormone measurements, and liver and testes were collected for histology, enzyme biochemistry, or gene expression profiling. Body and testis weights were unaffected, but liver weights were significantly increased by all four triazoles, and hepatocytes exhibited centrilobular hypertrophy. Myclobutanil exposure increased serum testosterone and decreased sperm motility, but no treatment-related testis histopathology was observed. We hypothesized that gene expression profiles would identify potential mechanisms of toxicity and used DNA microarrays and quantitative real-time PCR (qPCR) to generate profiles. Triazole fungicides are designed to inhibit fungal cytochrome P450 (CYP) 51 enzyme but can also modulate the expression and function of mammalian CYP genes and enzymes. Triazoles affected the expression of numerous CYP genes in rat liver and testis, including multiple Cyp2c and Cyp3a isoforms as well as other xenobiotic metabolizing enzyme (XME) and transporter genes. For some genes, such as Ces2 and Udpgtr2, all four triazoles had similar effects on expression, suggesting possible common mechanisms of action. Many of these CYP, XME and transporter genes are regulated by xeno-sensing nuclear receptors, and hierarchical clustering of CAR/PXR-regulated genes demonstrated the similarities of toxicogenomic responses in liver between all four triazoles and in testis between myclobutanil and triadimefon. Triazoles also affected expression of multiple genes involved in steroid hormone metabolism in the two tissues. Thus, gene expression profiles helped identify possible toxicological mechanisms of the triazole fungicides

  15. Association of Human Development Index with global bladder, kidney, prostate and testis cancer incidence and mortality.

    Science.gov (United States)

    Greiman, Alyssa K; Rosoff, James S; Prasad, Sandip M

    2017-12-01

    To describe contemporary worldwide age-standardized incidence and mortality rates for bladder, kidney, prostate and testis cancer and their association with development. We obtained gender-specific, age-standardized incidence and mortality rates for 184 countries and 16 major world regions from the GLOBOCAN 2012 database. We compared the mortality-to-incidence ratios (MIRs) at national and regional levels in males and females, and assessed the association with socio-economic development using the 2014 United Nations Human Development Index (HDI). Age-standardized incidence rates were 2.9 (bladder) to 7.4 (testis) times higher for genitourinary malignancies in more developed countries compared with less developed countries. Age-standardized mortality rates were 1.5-2.2 times higher in more vs less developed countries for prostate, bladder and kidney cancer, with no variation in mortality rates observed in testis cancer. There was a strong inverse relationship between HDI and MIR in testis (regression coefficient 1.65, R 2 = 0.78), prostate (regression coefficient -1.56, R 2 = 0.85), kidney (regression coefficient -1.34, R 2 = 0.74), and bladder cancer (regression coefficient -1.01, R 2 = 0.80). While incidence and mortality rates for genitourinary cancers vary widely throughout the world, the MIR is highest in less developed countries for all four major genitourinary malignancies. Further research is needed to understand whether differences in comorbidities, exposures, time to diagnosis, access to healthcare, diagnostic techniques or treatment options explain the observed inequalities in genitourinary cancer outcomes. © 2017 The Authors BJU International © 2017 BJU International Published by John Wiley & Sons Ltd.

  16. The Blood-Testis Barrier and Male Sexual Dysfunction following Spinal Cord Injury

    Science.gov (United States)

    2014-10-01

    Naive Single live cells (% ) 0 5 10 15 B cells T cells Neutrophils Dendritic cells Macrophages NK cells NKT cells Age-matched naive 1.5 year post-SCI...antigenic sperm and sperm cell -containing compartments within the testis. We also demonstrated that once failed, the BTB remains permeable, essentially...through mature) as well as sperm precursor/stem cells from the immune system. We reported that a contusive injury to the rat spinal cord causes a

  17. Identification of cancer/testis-antigen genes by massively parallel signature sequencing

    OpenAIRE

    Chen, Yao-Tseng; Scanlan, Matthew J.; Venditti, Charis A.; Chua, Ramon; Theiler, Gregory; Stevenson, Brian J.; Iseli, Christian; Gure, Ali O.; Vasicek, Tom; Strausberg, Robert L.; Jongeneel, C. Victor; Old, Lloyd J.; Simpson, Andrew J. G.

    2005-01-01

    Massively parallel signature sequencing (MPSS) generates millions of short sequence tags corresponding to transcripts from a single RNA preparation. Most MPSS tags can be unambiguously assigned to genes, thereby generating a comprehensive expression profile of the tissue of origin. From the comparison of MPSS data from 32 normal human tissues, we identified 1,056 genes that are predominantly expressed in the testis. Further evaluation by using MPSS tags from cancer cell lines and EST data fro...

  18. Aspiration and tetracycline sclerotherapy of primary vaginal hydrocoele of testis in adults

    International Nuclear Information System (INIS)

    Ali, J.; Anwar, W.

    2008-01-01

    Primary Vaginal Hydrocoele of Testis is a common condition which is primarily treated surgically. Many patients with Hydrocoele of testis are either not willing or are unfit for surgery. This study was done to know the safety, efficacy and out come of tetracycline induced sclerotherapy of Primary Vaginal Hydrocoele of Testis in adults. This quasi experimental study was done in Shahina Jamil Hospital, attached with Frontier Medical College and Ayub Teaching Hospital, Abbottabad from March 2006 to April, 2007. Thirty-seven patients with primary vaginal hydrocoele were included in the study. Aspiration and instillation of Tetracycline was done after spermatic cord block with 2% lignocaine. Procedure time, Peri and Post-procedure complications, number of injections for cure and patients satisfaction with the procedure were recorded. Patients were discharged home 3 to 4 hours after the procedure and followed up after one week, one month, three months and six months. Direct admission and re-admissions were recorded. The mean age of patients was 47 years. Mean procedure time was 45 minutes. All patients were cured. Mild postoprocedure pain occurred in 12 (40%), moderate pain in 14 (46%) patients and severe pain in 4 (13.3%) patients. No patient developed haematoma or local infection. One patient (3.3%) had micturition problem. Two (6.6%) patients had minimal recurrence. One injection was sufficient for cure in all patients, 28 (93%) patients were satisfied while 2 (6.6%) patients were not satisfied with this procedure. No patient was admitted in the hospital after the procedure. Aspiration and injection of tetracycline in Primary vaginal Hydrocoele of Testis in adults is safe, effective and very economical procedure. (author)

  19. The Blood Testis Barrier and Male Sexual Dysfunction following Spinal Cord Injury

    Science.gov (United States)

    2015-10-01

    privileged”. Our focus has been on the blood- spinal cord barrier (BSCB) and how trauma collapses this important spinal vascular specialization...AWARD NUMBER: W81XWH-12-1-0481 TITLE: The Blood-Testis Barrier and Male Sexual Dysfunction following Spinal Cord Injury PRINCIPAL INVESTIGATOR...21702-5012 Approved for Public Release; Distribution Unlimited Nearly 70% of males exhibit a profound loss of fertility following spinal cord injury

  20. Gene expression profiles in testis of pigs with extreme high and low levels of androstenone

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    Bendixen Christian

    2007-11-01

    Full Text Available Abstract Background: Boar taint is a major obstacle when using uncastrated male pigs for swine production. One of the main compounds causing this taint is androstenone, a pheromone produced in porcine testis. Here we use microarrays to study the expression of thousands of genes simultaneously in testis of high and low androstenone boars. The study allows identification of genes and pathways associated with elevated androstenone levels, which is essential for recognising potential molecular markers for breeding purposes. Results: Testicular tissue was collected from 60 boars, 30 with extreme high and 30 with extreme low levels of androstenone, from each of the two breeds Duroc and Norwegian Landrace. The samples were hybridised to porcine arrays containing 26,877 cDNA clones, detecting 563 and 160 genes that were differentially expressed (p Conclusion: This study contributes to the understanding of the complex genetic system controlling and responding to androstenone levels in pig testis. The identification of new pathways and genes involved in the biosynthesis and metabolism of androstenone is an important first step towards finding molecular markers to reduce boar taint.

  1. Amyloid precursor protein interaction network in human testis: sentinel proteins for male reproduction.

    Science.gov (United States)

    Silva, Joana Vieira; Yoon, Sooyeon; Domingues, Sara; Guimarães, Sofia; Goltsev, Alexander V; da Cruz E Silva, Edgar Figueiredo; Mendes, José Fernando F; da Cruz E Silva, Odete Abreu Beirão; Fardilha, Margarida

    2015-01-16

    Amyloid precursor protein (APP) is widely recognized for playing a central role in Alzheimer's disease pathogenesis. Although APP is expressed in several tissues outside the human central nervous system, the functions of APP and its family members in other tissues are still poorly understood. APP is involved in several biological functions which might be potentially important for male fertility, such as cell adhesion, cell motility, signaling, and apoptosis. Furthermore, APP superfamily members are known to be associated with fertility. Knowledge on the protein networks of APP in human testis and spermatozoa will shed light on the function of APP in the male reproductive system. We performed a Yeast Two-Hybrid screen and a database search to study the interaction network of APP in human testis and sperm. To gain insights into the role of APP superfamily members in fertility, the study was extended to APP-like protein 2 (APLP2). We analyzed several topological properties of the APP interaction network and the biological and physiological properties of the proteins in the APP interaction network were also specified by gene ontologyand pathways analyses. We classified significant features related to the human male reproduction for the APP interacting proteins and identified modules of proteins with similar functional roles which may show cooperative behavior for male fertility. The present work provides the first report on the APP interactome in human testis. Our approach allowed the identification of novel interactions and recognition of key APP interacting proteins for male reproduction, particularly in sperm-oocyte interaction.

  2. Prophylactic efficacy of Coriandrum sativum (Coriander) on testis of lead-exposed mice.

    Science.gov (United States)

    Sharma, Veena; Kansal, Leena; Sharma, Arti

    2010-09-01

    Lead poisoning is a worldwide health problem, and its treatment is under investigation. The aim of this study was to access the efficacy of Coriandrum sativum (coriander) in reducing lead-induced changes in mice testis. Animal exposed to lead nitrate showed significant decrease in testicular SOD, CAT, GSH, total protein, and tissue lead level. This was accompanied by simultaneous increase in the activities of LPO, AST, ALT, ACP, ALP, and cholesterol level. Serum testosterone level and sperm density were suppressed in lead-treated group compared with the control. These influences of lead were prevented by concurrent daily administration of C. sativum extracts to some extent. Treating albino mice with lead-induced various histological changes in the testis and treatment with coriander led to an improvement in the histological testis picture. The results thus led us to conclude that administration of C. sativum significantly protects against lead-induced oxidative stress. Further work need to be done to isolate and purify the active principle involved in the antioxidant activity of this plant.

  3. Identification of cancer/testis-antigen genes by massively parallel signature sequencing.

    Science.gov (United States)

    Chen, Yao-Tseng; Scanlan, Matthew J; Venditti, Charis A; Chua, Ramon; Theiler, Gregory; Stevenson, Brian J; Iseli, Christian; Gure, Ali O; Vasicek, Tom; Strausberg, Robert L; Jongeneel, C Victor; Old, Lloyd J; Simpson, Andrew J G

    2005-05-31

    Massively parallel signature sequencing (MPSS) generates millions of short sequence tags corresponding to transcripts from a single RNA preparation. Most MPSS tags can be unambiguously assigned to genes, thereby generating a comprehensive expression profile of the tissue of origin. From the comparison of MPSS data from 32 normal human tissues, we identified 1,056 genes that are predominantly expressed in the testis. Further evaluation by using MPSS tags from cancer cell lines and EST data from a wide variety of tumors identified 202 of these genes as candidates for encoding cancer/testis (CT) antigens. Of these genes, the expression in normal tissues was assessed by RT-PCR in a subset of 166 intron-containing genes, and those with confirmed testis-predominant expression were further evaluated for their expression in 21 cancer cell lines. Thus, 20 CT or CT-like genes were identified, with several exhibiting expression in five or more of the cancer cell lines examined. One of these genes is a member of a CT gene family that we designated as CT45. The CT45 family comprises six highly similar (>98% cDNA identity) genes that are clustered in tandem within a 125-kb region on Xq26.3. CT45 was found to be frequently expressed in both cancer cell lines and lung cancer specimens. Thus, MPSS analysis has resulted in a significant extension of our knowledge of CT antigens, leading to the discovery of a distinctive X-linked CT-antigen gene family.

  4. Congenital juvenile granulosa cell tumor of the testis: Case report and literature review

    Directory of Open Access Journals (Sweden)

    Carolina Talini

    2016-07-01

    Full Text Available Juvenile granulosa cell tumor (JGCT is a very rarely diagnosed benign tumor, accounting for 1.2% of all prepubertal testicular tumors. A full-term healthy neonate was diagnosed with a painless left scrotal mass. During evaluation it was identified to have about two times the volume of the contralateral testis, presenting a firm consistency, not as hard as the consistency of a prenatal testicular torsion. Doppler ultrasound detected a multicystic left testicular mass, with normal blood flow, but failed in detecting normal-appearing testis. Human chorionic gonadotropin (β-HCG and serum alpha-fetoprotein (AFP were normal. Inguinal approach was performed, section of the lesion was sent to frozen biopsy and excluded yolk sac tumor, and however the impossibility of detecting normal testis tissue indicated orchiectomy with high ligation of the spermatic cord. Histological evaluation demonstrated gray testicular parenchyma with multicystic aspect fulfilled with yellow fluid. The usual clinical presentation of JGCT is a painless scrotal mass, radiological imaging demonstrates a multicystic tumor. Tumoral markers levels are normal and the standard treatment is the inguinal orchiectomy.

  5. Sertoli cells maintain Leydig cell number and peritubular myoid cell activity in the adult mouse testis.

    Directory of Open Access Journals (Sweden)

    Diane Rebourcet

    Full Text Available The Sertoli cells are critical regulators of testis differentiation and development. In the adult, however, their known function is restricted largely to maintenance of spermatogenesis. To determine whether the Sertoli cells regulate other aspects of adult testis biology we have used a novel transgenic mouse model in which Amh-Cre induces expression of the receptor for Diphtheria toxin (iDTR specifically within Sertoli cells. This causes controlled, cell-specific and acute ablation of the Sertoli cell population in the adult animal following Diphtheria toxin injection. Results show that Sertoli cell ablation leads to rapid loss of all germ cell populations. In addition, adult Leydig cell numbers decline by 75% with the remaining cells concentrated around the rete and in the sub-capsular region. In the absence of Sertoli cells, peritubular myoid cell activity is reduced but the cells retain an ability to exclude immune cells from the seminiferous tubules. These data demonstrate that, in addition to support of spermatogenesis, Sertoli cells are required in the adult testis both for retention of the normal adult Leydig cell population and for support of normal peritubular myoid cell function. This has implications for our understanding of male reproductive disorders and wider androgen-related conditions affecting male health.

  6. Regulation of blood-testis barrier assembly in vivo by germ cells.

    Science.gov (United States)

    Li, Xiao-Yu; Zhang, Yan; Wang, Xiu-Xia; Jin, Cheng; Wang, Yu-Qian; Sun, Tie-Cheng; Li, Jian; Tang, Ji-Xin; Batool, Alia; Deng, Shou-Long; Chen, Su-Ren; Cheng, C Yan; Liu, Yi-Xun

    2018-01-03

    The assembly of the blood-testis barrier (BTB) during postnatal development is crucial to support meiosis. However, the role of germ cells in BTB assembly remains unclear. Herein, Kit W /Kit WV mice were used as a study model. These mice were infertile, failing to establish a functional BTB to support meiosis due to c-Kit mutation. Transplantation of undifferentiated spermatogonia derived from normal mice into the testis of Kit W /Kit WV mice triggered functional BTB assembly, displaying cyclic remodeling during the epithelial cycle. Also, transplanted germ cells were capable of inducing Leydig cell testosterone production, which could enhance the expression of integral membrane protein claudin 3 in Sertoli cells. Early spermatocytes were shown to play a vital role in directing BTB assembly by expressing claudin 3, which likely created a transient adhesion structure to mediate BTB and cytoskeleton assembly in adjacent Sertoli cells. In summary, the positive modulation of germ cells on somatic cell function provides useful information regarding somatic-germ cell interactions.-Li, X.-Y., Zhang, Y., Wang, X.-X., Jin, C., Wang, Y.-Q., Sun, T.-C., Li, J., Tang, J.-X., Batool, A., Deng, S.-L., Chen, S.-R., Cheng, C. Y., Liu, Y.-X. Regulation of blood-testis barrier assembly in vivo by germ cells.

  7. Tudor Domain Containing Protein TDRD12 Expresses at the Acrosome of Spermatids in Mouse Testis

    Directory of Open Access Journals (Sweden)

    Min Kim

    2016-07-01

    Full Text Available Tdrd12 is one of tudor domain containing (Tdrd family members. However, the expression pattern of Tdrd12 has not been well studied. To compare the expression levels of Tdrd12 in various tissues, real time-polymerase chain reaction was performed using total RNAs from liver, small intestine, heart, brain, kidney, lung, spleen, stomach, uterus, ovary, and testis. Tdrd12 mRNA was highly expressed in testis. Antibody against mouse TDRD12 were generated using amino acid residues SQRPNEKPLRLTEKKDC of TDRD12 to investigate TDRD12 localization in testis. Immunostaining assay shows that TDRD12 is mainly localized at the spermatid in the seminiferous tubules of adult testes. During postnatal development, TDRD12 is differentially expressed. TDRD12 was detected in early spermatocytes at 2 weeks and TDRD12 was localized at acrosome of the round spermatids. TDRD12 expression was not co-localized with TDRD1 which is an important component of piRNA pathway in germ cells. Our results indicate that TDRD12 may play an important role in spermatids and function as a regulator of spermatogenesis in dependent of TDRD1.

  8. Intravaginal testicular torsion in newborns. To fix or not to fix the contralateral testis?

    Directory of Open Access Journals (Sweden)

    G. Bordin

    2013-10-01

    Full Text Available Scrotal swelling suggesting testicular torsion is a rare urological emergency which requires a clinical urgent evaluation and most of the times must be managed surgically. In newborns it can occur in the postnatal period, usually within the twenty-eighth day of life, or more frequently in utero, during the descent of the testis into the scrotum. Usually its poor fixedness allows the testis an abnormal mobility inside the scrotum, configuring the framework of extravaginal torsion. On the contrary during the perinatal period a twist that takes place inside the tunica vaginalis, known as intravaginal torsion, is extremely uncommon and only few cases are well documented in the literature. Authors present a rare case of intravaginal testicular torsion occurred in perinatal period. In this situation only the early surgical exploration of the scrotum may allow the rescue of the gonad, although in rare cases. Timing of surgical treatment and need for contralateral testicular fixation remain controversial. However since the anatomical defect of the tunica vaginalis can be bilateral the surgical fixation even of the contralateral testis is important, now or later, in order to prevent any future torsion of this gonad. The authors also present a brief review of recent literature on the subject.

  9. Histopathological evaluation of the Zebrafish (Danio rerio testis following exposure to methyl paraben

    Directory of Open Access Journals (Sweden)

    Nasrin Hassanzadeh

    2017-04-01

    Full Text Available Methyl paraben (MP is widely used as a preservative in various products. It frequently enters into aquatic environment and renders potential threat to fish. The aim of this study was to evaluate reproductive toxicity of MP on zebrafish (Danio rerio under laboratory conditions. Male zebrafish were exposed to four concentrations of MP (0.001, 0.01, 1, and 10 mg L-1 for 21 days in semi-static condition. Changes in mean length, mean weight, gonadosomatic index (GSI and histology of testis were studied. Treatment at 0.001 to 10 mg L-1 MP had no significant effect on the survival, mean length and mean weight of fish. But, GSI decreased in a dose dependent manner and the decrease was significant in the group that received the highest dose. Histological alteration of testis consisted of general testicular atrophy, multi-nucleated gonocytes (MNGs, impaired germ cell, spermatogonial proliferation, Leydig cell hyperplasia, interstitial fibrosis and apoptosis of Sertoli cells. It was concluded that sub-chronic exposures of MP could adversely affect GSI, disrupt the histology of testis and produce estrogenic and antispermatogenic activity in male zebra fish.

  10. Lightning and Radar Observations of the 29 May 2004 Tornadic HP Supercell during TELEX

    Science.gov (United States)

    Macgorman, D. R.; Kuhlman, K. M.

    2006-12-01

    On 29 May 2004, a tornadic heavy-precipitation (HP) supercell storm moved through central Oklahoma and through the Thunderstorm Electrification and Lightning Experiment (TELEX) domain. Three dimensional lightning location data from the Oklahoma Lightning Mapping Array (OK-LMA) depict the evolution of the storm, including convective surges, overshooting tops, and the formation and dissipation of lightning weak holes. In addition to the OK-LMA, the storm was also observed by two C-band mobile radars (SMART-R radars) providing three-minute volume scans and by the KOUN polarimetric S-band radar. Analysis of a lightning weak hole showed that it was co-located horizontally with a core of strong updrafts and with a bounded weak echo region. The majority of the cloud-to-ground lightning detected in the storm by the National Lightning Detection Network lowered negative charge to ground. However, during genesis of the strongest tornado, the dominant polarity of ground flashes near the reflectivity core of the storm evolved to positive. Also at approximately this time, negative ground strikes began occurring under the anvil, tens of kilometers from the reflectivity core, as lightning activity surged roughly 100 km into the anvil. Observations from these platforms provide insight into HP supercell evolution and structure and into relationships of lightning with other properties of the storm.

  11. Multi-level hp-finite cell method for embedded interface problems with application in biomechanics.

    Science.gov (United States)

    Elhaddad, Mohamed; Zander, Nils; Bog, Tino; Kudela, László; Kollmannsberger, Stefan; Kirschke, Jan; Baum, Thomas; Ruess, Martin; Rank, Ernst

    2017-12-19

    This work presents a numerical discretization technique for solving 3-dimensional material interface problems involving complex geometry without conforming mesh generation. The finite cell method (FCM), which is a high-order fictitious domain approach, is used for the numerical approximation of the solution without a boundary-conforming mesh. Weak discontinuities at material interfaces are resolved by using separate FCM meshes for each material sub-domain and weakly enforcing the interface conditions between the different meshes. Additionally, a recently developed hierarchical hp-refinement scheme is used to locally refine the FCM meshes to resolve singularities and local solution features at the interfaces. Thereby, higher convergence rates are achievable for nonsmooth problems. A series of numerical experiments with 2- and 3-dimensional benchmark problems is presented, showing that the proposed hp-refinement scheme in conjunction with the weak enforcement of the interface conditions leads to a significant improvement of the convergence rates, even in the presence of singularities. Finally, the proposed technique is applied to simulate a vertebra-implant model. The application showcases the method's potential as an accurate simulation tool for biomechanical problems involving complex geometry, and it demonstrates its flexibility in dealing with different types of geometric description. Copyright © 2017 John Wiley & Sons, Ltd.

  12. Diversification of HP1-like Chromo Domain Proteins in Tetrahymena thermophila.

    Science.gov (United States)

    Wiley, Emily A; Horrell, Scott; Yoshino, Alyssa; Schornak, Cara C; Bagnani, Claire; Chalker, Douglas L

    2018-01-01

    Proteins that possess a chromo domain are well-known for their roles in heterochromatin assembly and maintenance. The Heterochromatin Protein 1 (HP1) family, with a chromo domain and carboxy-terminal chromo shadow domain, targets heterochromatin through interaction with histone H3 methylated on lysine 9 (H3K9me2/3). The structural and functional diversity of these proteins observed in both fission yeast and metazoans correlate with chromatin specialization. To expand these studies, we examined chromo domain proteins in the ciliate Tetrahymena thermophila, which has functionally diverse and developmentally regulated heterochromatin domains. We identified thirteen proteins similar to HP1. Together they possess only a fraction of the possible chromo domain subtypes and most lack a recognizable chromo shadow domain. Using fluorescence microscopy to track chromatin localization of tagged proteins through the life cycle, we show evidence that in T. thermophila this family has diversified with biological roles in RNAi-directed DNA elimination, germline genome structure, and somatic heterochromatin. Those proteins with H3K27me3 binding sequence characteristics localize to chromatin in mature nuclei, whereas those with H3K9me2/3 binding characteristics localize to developing nuclei undergoing DNA elimination. Findings point to an expanded and diversified family of chromo domain proteins that parallels heterochromatin diversity in ciliates. © 2017 The Authors. Journal of Eukaryotic Microbiology published by Wiley Periodicals, Inc. on behalf of International Society of Protistologists.

  13. A replica exchange Monte Carlo algorithm for protein folding in the HP model

    Directory of Open Access Journals (Sweden)

    Shmygelska Alena

    2007-09-01

    Full Text Available Abstract Background The ab initio protein folding problem consists of predicting protein tertiary structure from a given amino acid sequence by minimizing an energy function; it is one of the most important and challenging problems in biochemistry, molecular biology and biophysics. The ab initio protein folding problem is computationally challenging and has been shown to be NP MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaat0uy0HwzTfgDPnwy1egaryqtHrhAL1wy0L2yHvdaiqaacqWFneVtcqqGqbauaaa@3961@-hard even when conformations are restricted to a lattice. In this work, we implement and evaluate the replica exchange Monte Carlo (REMC method, which has already been applied very successfully to more complex protein models and other optimization problems with complex energy landscapes, in combination with the highly effective pull move neighbourhood in two widely studied Hydrophobic Polar (HP lattice models. Results We demonstrate that REMC is highly effective for solving instances of the square (2D and cubic (3D HP protein folding problem. When using the pull move neighbourhood, REMC outperforms current state-of-the-art algorithms for most benchmark instances. Additionally, we show that this new algorithm provides a larger ensemble of ground-state structures than the existing state-of-the-art methods. Furthermore, it scales well with sequence length, and it finds significantly better conformations on long biological sequences and sequences with a provably unique ground-state structure, which is believed to be a characteristic of real proteins. We also present evidence that our REMC algorithm can fold sequences which exhibit significant interaction between termini in the hydrophobic core relatively easily. Conclusion We demonstrate that REMC utilizing the pull move

  14. Proposal of concentration limits for determining the hazard property HP 14 for waste using ecotoxicological tests.

    Science.gov (United States)

    Hennebert, Pierre

    2018-04-01

    Different ecotoxicological test batteries and concentration limits have been proposed to assess the hazard property (HP) HP 14 'Ecotoxic' for waste in the European Union and its member states. In test batteries, if the concentration of waste in the culture/dilution medium producing 50% of inhibitory biological effect in one or more test(s) is below the concentration limit of the test, the waste is classified as hazardous. A summarized review of the test batteries proposed since 1998 is presented. The last proposed test battery uses seven aquatic and terrestrial species with standardized methods, but with options and uniform concentration limits of 10% of waste eluate or solid waste in the culture/dilution medium. No attempt was made to match this hazard assessment with the classification made in the European List of Waste (LoW). The aim of this paper is to propose for the same test battery (reduced to 6 tests without options) concentration limits that match with the European List of Waste. This list was taken as reference (despite the fact that waste can be hazardous for other properties than the most frequent HP 14, and its partly political nature for some opinions). The concentration limits (CLs) for tests are the concentrations producing the highest ecotoxicological effects for each test observed in a non-hazardous waste set. Data from Germany, France and Belgium (from in total 5 different sources from 2009 to 2016) with the above-mentioned test battery (without options) were gathered for 81 samples, being the largest set ever published. In total, ten non-hazardous (NH) waste samples (as defined by the LoW and for most of them checked by chemical composition) were used to establish CLs. These CLs were then applied to 13 hazardous (H) waste by the LoW, and all were classified as hazardous. The matching of the resulting classification with the LoW is convincing. For the 58 'mirror entries' in the LoW (hazardous or not depending of the presence of hazardous

  15. Risk stratification for surgical outcomes in older colorectal cancer patients using ISAR-HP and G8 screening tools.

    Science.gov (United States)

    Souwer, Esteban T D; Verweij, Norbert M; van den Bos, Frederiek; Bastiaannet, Esther; Slangen, Rob M E; Steup, Willem H; Hamaker, Marije E; Portielje, Johanna E A

    2018-03-01

    Older patients are at risk for adverse outcomes after surgical treatment of cancer. Identifying patients at risk could affect treatment decisions and prevent functional decline. Screening tools are available to select patients for Geriatric Assessment. Until now their predictive value for adverse outcomes in older colorectal cancer patients has not been investigated. To study the predictive value of the Geriatric 8 (G8) and Identification of Seniors at Risk for Hospitalized Patients (ISAR-HP) screening tools for adverse outcomes after elective colorectal surgery in patients older than 70years. Primary outcomes were 30-day complication rates, secondary outcomes were the length of hospital stay and six-month mortality. Multicentre cohort study from two hospitals in the Netherlands. Frail was defined as a G8 ≤14 and/or ISAR-HP ≥2. Odds ratio (OR) is given with 95% CI. Overall, 139 patients (52%) out of 268 patients were included; 32 patients (23%) were ISAR-HP-frail, 68 (50%) were G8-frail, 20 were frail on both screening tools. Median age was 77.7years. ISAR-HP frail patients were at risk for 30-day complications OR 2.4 (CI 1.1-5.4, p=0.03), readmission OR 3.4 (1.1-11.0), cardiopulmonary complications OR 5.9 (1.6-22.6), longer hospital stay (10.3 versus 8.9day) and six-months mortality OR 4.9 (1.1-23.4). When ISAR-HP and G8 were combined OR increased for readmission, 30-day and six-months mortality. G8 alone had no predictive value. ISAR-HP-frail patients are at risk for adverse outcomes after colorectal surgery. ISAR-HP combined with G8 has the strongest predictive value for complications and mortality. Patients screening frail on ISAR-HP are at increased risk for morbidity and mortality. Screening results of G8 alone was not predictive for postoperative outcomes. Predictive value increased when G8 and ISAR-HP were combined. Copyright © 2017 Elsevier Inc. All rights reserved.

  16. [In vivo and in vitro effect of peptide HP-6 derived from donkey serum albumin on hematopoietic system].

    Science.gov (United States)

    Li, Hao; Yan, Shengmin; Wang, Zhefeng; Shen, Xiaomiao; Rao, Pingfan

    2011-08-01

    By bioinformatics method, the effect in hematopoietic system of bioactive peptide HP-6, which was obtained from donkey serum albumin and is one of the major protein components from donkey-hide gelatin, was investigated. Human bone marrow nucleated cells (hBMNCs) and murine bone marrow stromal cells (mBMSCs) were separated and cultured with different concentration of peptide HP-6 (0.000 15, 0.001 5, 0.015, 0.15, 1.5 micromol x L(-1)). The effect on promoting proliferation of cells related to hematopoiesis in bone morrow was detected and the ultrastructure of cells after treated by HP-6 was observed through transmission electron microscope. Hemorrhage anemia mouse model and anemia mouse model induced by cyclophosphamide were established, and randomly divided into peptide HP-6 groups which were administered respectively with different doses (1, 0.1, 0.01 mg x kg(-1)) by gavage, and control group which was administered with PBS by gavage. Peripheral blood components of all mice and bone morrow cells (BMC) number of mice induced by cyclophosphamide were evaluated. Peptide HP-6 could concentration-related promote the proliferation of hBMNCs and mBMSCs, hBMNCs got the highest reproduction rate of 152.11% and mBMSCs also got 63.52% with the concentration of 0.15 micromol x L(-1), then the reproduction rate decreased while the concentration kept increasing. The transmission electron microscope showed that ultrastructure of cells was normal after treated by HP-6.1 mg x kg(-1) peptide HP-6 significantly increased peripheral platelet and protected mouse morrow injured by cyclophoshamide. 0.1 mg x kg(-1) peptide HP-6 significantly increased peripheral platelet with relative growth rate of 77.65%, increased peripheral white blood cells count and peripheral red blood cells count, also could protect mouse peripheral blood after treated by chemotherapeutics. Peptide HP-6 could promote the proliferation of cells related to hematopoietic system, enhance mouse hemopoiesis function and

  17. Bensal HP for Second Intention Healing Following Mohs Micrographic Surgery or Shave Skin Biopsy: An Open-label Pilot Study.

    Science.gov (United States)

    Mordorski, Breanne; Friedman, Adam J; Kircik, Leon H

    2016-10-01

    Dermatologists frequently create cutaneous defects that heal by second intention, yet there is no universal protocol for wound care in this setting. Several ointments commonly used for wound healing are not cost effective as they contain known contact allergens, contribute to antimicrobial resistance, and do not enhance the healing process. Recent studies indicate that Bensal HP, a commercially available ointment used for a variety of dermatologic conditions, may be useful for wound healing; although clinical data is currently limited. In this single-center open-label pilot study, Bensal HP was evaluated for second intention healing over 8 weeks following either Mohs micrographic surgery or shave skin biopsy in 20 patients. Results indicate that Bensal HP is effective for second intention healing as demonstrated by increased Global Assessment of Efficacy scores and decreased wound measurements, with 16 patients achieving full closure. Patient symptoms overall improved over the study period, and Bensal HP was well tolerated with no adverse effects associated with its use. By providing critical data regarding the safety and efficacy of Bensal HP, this study may provide useful information to guide further assessment in future large-scale comparative wound healing studies. J Drugs Dermatol. 2016;15(10):1197-1202.

  18. Linear epitope recognition antibodies strongly respond to the C-terminal domain of HP-PRRSV GP5.

    Science.gov (United States)

    Wang, Xinglong; Qui, Li; Dang, Yu; Xiao, Sha; Zhang, Shuxia; Yang, Zengqi

    2014-12-05

    A total of 155 peptides derived from the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) glycoprotein 5 (GP5) were printed on a chip to reveal the antigen reaction characteristics of the protein. The reactions of these peptides to HP-PRRSV-specific pig serum were scanned and quantified using fluorescence intensity via the PepSlide(®) Analyzer software. The intensity plots showed different reactions in the different sectors of GP5. The highest reaction intensity value reached 3894.5, with a peptide sequence of IVEKGGKVEVEGHLI. Seventeen peptides that showed relatively high reaction levels with HP-PRRSV-specific pig serum were selected as epitope candidates. Furthermore, the antigenic character was predicted using a software and was compared with the peptide scan results. In contrast to the software prediction, the HP-PRRSV-specific antibodies strongly responded to the C-terminal domain of GP5. The acquired data may be useful for understanding the antigenic characteristics of HP-PRRSV GP5. Copyright © 2014 Elsevier B.V. All rights reserved.

  19. Goal-Oriented Self-Adaptive hp Finite Element Simulation of 3D DC Borehole Resistivity Simulations

    KAUST Repository

    Calo, Victor M.

    2011-05-14

    In this paper we present a goal-oriented self-adaptive hp Finite Element Method (hp-FEM) with shared data structures and a parallel multi-frontal direct solver. The algorithm automatically generates (without any user interaction) a sequence of meshes delivering exponential convergence of a prescribed quantity of interest with respect to the number of degrees of freedom. The sequence of meshes is generated from a given initial mesh, by performing h (breaking elements into smaller elements), p (adjusting polynomial orders of approximation) or hp (both) refinements on the finite elements. The new parallel implementation utilizes a computational mesh shared between multiple processors. All computational algorithms, including automatic hp goal-oriented adaptivity and the solver work fully in parallel. We describe the parallel self-adaptive hp-FEM algorithm with shared computational domain, as well as its efficiency measurements. We apply the methodology described to the three-dimensional simulation of the borehole resistivity measurement of direct current through casing in the presence of invasion.

  20. Mutantes rin, norA, og c e hp em diferentes backgrounds genotípicos de tomateiro Rin, norA, og c and hp mutants in tomatos with different genetic backgrounds

    Directory of Open Access Journals (Sweden)

    Marcos Ventura Faria

    2006-05-01

    Full Text Available O objetivo deste trabalho foi avaliar a viabilidade do emprego isolado e simultâneo dos mutantes rin, norA, og c e hp em heterozigose, em genótipos de tomateiro, visando à melhoria da qualidade pós-colheita dos frutos. Foram avaliados 18 genótipos que diferem quanto às combinações entre estes locos em dois diferentes backgrounds (FloraDade e background experimental. Contrastes não ortogonais foram estabelecidos para quantificar os efeitos dos alelos mutantes, isoladamente ou combinados em um mesmo genótipo, em dois backgrounds, sobre a produção total e produção precoce de frutos, massa média, firmeza, coloração externa, teores de licopeno e betacaroteno dos frutos. Os alelos norA e rin em heterozigose, no background FloraDade, desaceleraram a taxa de perda de firmeza e reduziram os teores de licopeno e betacaroteno nos frutos maduros. As combinações heterozigotas entre o mutante rin e os mutantes norA, og c e hp aumentaram a firmeza dos frutos. O efeito do genótipo rin+/rin nor+/norA sobre a firmeza dos frutos foi o somatório dos efeitos individuais dos locos. O alelo rin mostrou-se, individualmente, mais eficiente do que norA, em prolongar a firmeza dos frutos. Os genótipos og c+/og c e hp+/hp, juntos ou isolados, aumentaram a coloração dos frutos rin+/rin. Recomenda-se a utilização dos genótipos rin+/rin nor+/norA no desenvolvimento de híbridos longa vida, buscando-se, contudo, backgrounds que sofram prejuízo menor sobre a coloração dos frutos e adicionando-se mutantes og c+/og c e hp+/hp.The objective of this work was to assess the viability of single and simultaneous use of rin, norA, og c and hp as heterozygotes in tomato genotypes in order to improve post-harvest fruit quality. Eighteen genotypes differing in combinations among these loci in two backgrounds (Floradade and experimental background were evaluated. Contrasts were used to quantify the effects of single and simultaneous application of mutant loci

  1. Expressed sequence tags from Peromyscus testis and placenta tissue: Analysis, annotation, and utility for mapping

    Directory of Open Access Journals (Sweden)

    Bullard-Dillard Rebecca

    2008-06-01

    Full Text Available Abstract Background Mice of the genus Peromyscus are found in nearly every habitat from Alaska to Central America and from the Atlantic to the Pacific. They provide an evolutionary outgroup to the Mus/Rattus lineage and serve as an intermediary between that lineage and humans. Although Peromyscus has been studied extensively under both field and laboratory conditions, research has been limited by the lack of molecular resources. Genes associated with reproduction typically evolve rapidly and thus are excellent sources of evolutionary information. In this study we describe the generation of two cDNA libraries, one from placenta and one from testis, characterize the resulting ESTs, and describe their utility for mapping the Peromyscus genome. Results The 5' ends of 1,510 placenta and 4,798 testis clones were sequenced. Low quality sequences were removed and after clustering and contig assembly, 904 unique placenta and 2,002 unique testis sequences remained. Average lengths of placenta and testis ESTs were 711 bp and 826 bp, respectively. Approximately 82% of all ESTs were identified using the BLASTX algorithm to Mus and Rattus, and 34 – 54% of all ESTs could be assigned to a biological process gene ontology category in either Mus or Rattus. Because the Peromyscus genome organization resembles the Rattus genome more closely than Mus we examined the distribution of the Peromyscus ESTs across the rat genome finding markers on all rat chromosomes except the Y. Approximately 40% of all ESTs were specific to only one location in the Mus genome and spanned introns of an appropriate size for sequencing and SNP detection. Of the primers that were tried 54% provided useful assays for genotyping on interspecific backcross and whole-genome radiation hybrid cell panels. Conclusion The 2,906 Peromyscus placenta and testis ESTs described here significantly expands the molecular resources available for the genus. These ESTs allow for specific PCR amplification

  2. Ascending testis after repair of pediatric inguinal hernia and hydrocele: A misunderstood operative complication.

    Science.gov (United States)

    Wang, Furan; Zhong, Hongji; Zhao, Junfeng

    2017-02-01

    Ascending testis (AT) is a rare complication after repair of an inguinal hernia/hydrocele. However, there has been some controversy concerning the AT following laparoscopic and open procedures. To review the experience of, and discuss the associated mechanisms with, testicular ascent after pediatric inguinal hernia/hydrocele surgery. A retrospective review of the medical records of male children who underwent inguinal hernia/hydrocele repair at the present hospital between January 2000 and December 2014. Those who underwent subsequent orchiopexies due to ipsilateral cryptorchidism were identified. The ATs that were misdiagnosed, caused by improper operation, and retractile testes were excluded. The Poisson distribution evaluated the incidences of subgroups. A total of 17,295 inguinal hernias and hydroceles were repaired on 12,849 males; of whom, 10 testes (0.058%) developed ATs on nine individuals postoperatively (Summary Table). The difference of AT incidences between subgroups was insignificant (P > 0.05), except for that grouped by the age at initial operation (cutoff = 1 year, P = 0.008; cutoff = 2 years, P = 0.012). During orchiopexy, extensive adhesions were found in the inguinal canal only in the two cases following open repair. The hernia sac/processus vaginalis remained intact in the canal of AT after laparoscopic repair, and partial after open herniotomy. It was generally assumed that testicular ascent after repair of an inguinal hernia/hydrocele was caused by adhesion of the spermatic cord. However, the cord was not dissected during laparoscopic procedure, so adhesion was not the major reason for AT following laparoscopic surgery. The sac/processus were partially excised during open repair, but kept intact in laparoscopic procedure. Therefore, remnants of the sac/processus might play a greater role in postoperative testicular ascent than adhesions. Furthermore, it was found that AT incidence after the repair was not higher than that in 'normal

  3. Hp(3)/Φ conversion coefficients for neutrons: discussion on the basis of the new ICRP recommended limit for the eye lens.

    Science.gov (United States)

    Ferrari, P; Gualdrini, G; Tanner, R; Fantuzzi, E

    2014-10-01

    The new recommendation issued by the International Commission on Radiological Protection (ICRP) introducing a 20-mSv annual dose limit for the eye lens stimulated an interesting debate among the radiation protection community. In the present work the problem of estimating Hp(3) for neutron realistic workplace spectra is treated, employing the recently published Hp(3)/Φ conversion coefficients with the aim of establishing a workplace-dependent relationship between Hp(10) and Hp(3). The results demonstrate that, whilst the two quantities can differ by less than 10 %, in general, Hp(10) cannot be considered a conservative estimate of Hp(3). © The Author 2013. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  4. Effect of a long-chained fructan Raftiline HP on blood lipids and spontaneous atherosclerosis in low density receptor knockout mice

    DEFF Research Database (Denmark)

    Mortensen, Alicja; Poulsen, Morten; Frandsen, Henrik Lauritz

    2002-01-01

    The effect of a long-chained fructan Raftiline HP on spontaneous hypercholesterolemia and atherosclerosis was studied in 40 LDLR-/- male mice receiving isocaloric, balanced in fat content, purified diets with 0 or 10% Raftiline HP, for 16 weeks. The feed intake was comparable (3.9 v. 3.8 g....../day) but the terminal body weight was lower in the Raftiline HP group (36 v. 32g, p...

  5. Improved Functional Properties and Efficiencies of Nitinol Wires Under High-Performance Shape Memory Effect (HP-SME)

    Science.gov (United States)

    Casati, R.; Saghafi, F.; Biffi, C. A.; Vedani, M.; Tuissi, A.

    2017-10-01

    Martensitic Ti-rich NiTi intermetallics are broadly used in various cyclic applications as actuators, which exploit the shape memory effect (SME). Recently, a new approach for exploiting austenitic Ni-rich NiTi shape memory alloys as actuators was proposed and named high-performance shape memory effect (HP-SME). HP-SME is based on thermal recovery of de-twinned martensite produced by mechanical loading of the parent phase. The aim of the manuscript consists in evaluating and comparing the fatigue and actuation properties of austenitic HP-SME wires and conventional martensitic SME wires. The effect of the thermomechanical cycling on the actuation response and the changes in the electrical resistivity of both shape memory materials were studied by performing the actuation tests at different stages of the fatigue life. Finally, the changes in the transition temperatures before and after cycling were also investigated by differential calorimetric tests.

  6. New Diphosphopentamolybdate K5[HP2Mo5O23] · 10H2O: Synthesis, Structure, and Characterization

    Science.gov (United States)

    Harchani, Ali; Haddad, A.

    2017-12-01

    A new diphosphopentamolybdate(VI) K5[HP2Mo5O23] · 10H2O has been prepared employing solution method and characterized by single crystal X-ray diffraction, energy dispersive analysis, infrared spectroscopy, thermal stability analysis, cyclic voltammetry analysis, and ultraviolet visible spectroscopy. The compound crystallizes in the orthorhombic sp. gr. Pcab: a = 15.9661(10) Å, b = 19.0832(10) Å, c = 20.0970(9) Å, V = 6123.27(10) Å3, Z = 8. The crystal structure contains the polyanion units [HP2Mo5O23]5- around the potassium cations and lattice water. The [HP2Mo5O23]5- subunits are connected together through K-O-Mo bridges and hydrogen bonds. The crystal shape morphology was simulated using the Bravais-Friedel-Donnay-Harker model.

  7. User manual of the multicompenent variably - saturated flow and transport model HP1

    International Nuclear Information System (INIS)

    Jacques, D.; Simunek, J.

    2005-06-01

    This report describes a new comprehensive simulation tool HP1 (HYDRUS1D-PHREEQC) that was obtained by coupling the HYDRUS-1D one-dimensional variably-saturated water flow and solute transport model with the PHREEQC geochemical code. The HP1 code incorporates modules simulating (1) transient water flow in variably-saturated media, (2) transport of multiple components, and (3) mixed equilibrium/kinetic geochemical reactions. The program numerically solves the Richards equation for variably-saturated water flow and advection-dispersion type equations for heat and solute transport. The flow equation incorporates a sink term to account for water uptake by plant roots. The heat transport equation considers transport due to conduction and convection with flowing water. The solute transport equations consider advective-dispersive transport in the liquid phase. The program can simulate a broad range of low-temperature biogeochemical reactions in water, soil and ground water systems including interactions with minerals, gases, exchangers, and sorption surfaces, based on thermodynamic equilibrium, kinetics, or mixed equilibrium-kinetic reactions. The program may be used to analyze water and solute movement in unsaturated, partially saturated, or fully saturated porous media. The flow region may be composed of nonuniform soils or sediments. Flow and transport can occur in the vertical, horizontal, or a generally inclined direction. The water flow part of the model can deal with prescribed head and flux boundaries, boundaries controlled by atmospheric conditions, as well as free drainage boundary conditions. The governing flow and transport equations were solved numerically using Galerkin-type linear finite element schemes. To test the accuracy of the coupling procedures implemented in HP1, simulation results were compared with (i) HYDRUS-1D for transport problems of multiple components subject to sequential first-order decay, (ii) PHREEQC for steady-state flow conditions, and

  8. The WRKY transcription factor HpWRKY44 regulatesCytP450-like1expression in red pitaya fruit (Hylocereus polyrhizus).

    Science.gov (United States)

    Cheng, Mei-Nv; Huang, Zi-Juan; Hua, Qing-Zhu; Shan, Wei; Kuang, Jian-Fei; Lu, Wang-Jin; Qin, Yong-Hua; Chen, Jian-Ye

    2017-01-01

    Red pitaya ( Hylocereus polyrhizus ) fruit is a high-value, functional food, containing a high level of betalains. Several genes potentially related to betalain biosynthesis, such as cytochrome P450-like ( CytP450-like ), have been identified in pitaya fruit, while their transcriptional regulation remains unclear. In this work, the potential involvement of a WRKY transcription factor, HpWRKY44, in regulating CytP450-like1 expression in pitaya fruit was examined. HpWRKY44, a member of the Group 1 WRKY family, contains two conserved WRKY motifs and is localized in the nucleus. HpWRKY44 also exhibits trans-activation ability. Gene expression analysis showed that the expression of HpCytP450-like1 and HpWRKY44 increased steadily during pitaya fruit coloration, which corresponded with the production of elevated betalain levels in the fruit. HpWRKY44 was also demonstrated to directly bind to and activate the HpCytP450-like1 promoter via the recognition of the W-box element present in the promoter. Collectively, our findings indicate that HpWRKY44 transcriptionally activates HpCytP450-like1 , which perhaps, at least in part, contributes to betalain biosynthesis in pitaya fruit. The information provided in the current study provides novel insights into the regulatory network associated with betalain biosynthesis during pitaya fruit coloration.

  9. Helicobacter pylori antigen HP0986 (TieA) interacts with cultured gastric epithelial cells and induces IL8 secretion via NF-κB mediated pathway.

    Science.gov (United States)

    Devi, Savita; Ansari, Suhail A; Vadivelu, Jamuna; Mégraud, Francis; Tenguria, Shivendra; Ahmed, Niyaz

    2014-02-01

    The envisaged roles and partly understood functional properties of Helicobacter pylori protein HP0986 are significant in the context of proinflammatory and or proapoptotic activities, the two important facilitators of pathogen survival and persistence. In addition, sequence analysis of this gene predicts a restriction endonuclease function which remained unknown thus far. To evaluate the role of HP0986 in gastric inflammation, we studied its expression profile using a large number of clinical isolates but a limited number of biopsies and patient sera. Also, we studied antigenic role of HP0986 in altering cytokine responses of human gastric epithelial (AGS) cells including its interaction with and localization within the AGS cells. For in vitro expression study of HP0986, 110 H. pylori clinical isolates were cultured from patients with functional dyspepsia. For expression analysis by qRT PCR of HP0986, 10 gastric biopsy specimens were studied. HP0986 was also used to detect antibodies in patient sera. AGS cells were incubated with recombinant HP0986 to determine cytokine response and NF-κB activation. Transient transfection with HP0986 cloned in pEGFPN1 was used to study its subcellular localization or homing in AGS cells. Out of 110 cultured H. pylori strains, 34 (31%) were positive for HP0986 and this observation was correlated with in vitro expression profiles. HP0986 mRNA was detected in 7 of the 10 biopsy specimens. Further, HP0986 induced IL-8 secretion in gastric epithelial cells in a dose and time-dependent manner via NF-κB pathway. Serum antibodies against HP0986 were positively associated with H. pylori positive patients. Transient transfection of AGS cells revealed both cytoplasmic and nuclear localization of HP0986. HP0986 was moderately prevalent in clinical isolates and its expression profile in cultures and gastric biopsies points to its being naturally expressed. Collective observations including the induction of IL-8 via TNFR1 and NF

  10. [(68) Ga]-HP-DO3A-nitroimidazole: a promising agent for PET detection of tumor hypoxia.

    Science.gov (United States)

    Wu, Yunkou; Hao, Guiyang; Ramezani, Saleh; Saha, Debabrata; Zhao, Dawen; Sun, Xiankai; Sherry, A Dean

    2015-01-01

    The goal of this study is to evaluate a new (68) Ga-based imaging agent for detecting tumor hypoxia using positron emission tomography (PET). The new hypoxia targeting agent reported here, [(68) Ga]-HP-DO3A-nitroimidazole ([(68) Ga]-HP-DO3A-NI), was constructed by linking a nitroimidazole moiety with the macrocyclic ligand component of ProHance®, HP-DO3A. The hypoxia targeting capability of this agent was evaluated in A549 lung cancer cells in vitro and in SCID mice bearing subcutaneous A549 tumor xenografts. The cellular uptake assays showed that significantly more [(68) Ga]-HP-DO3A-NI accumulates in hypoxic tumor cells at 30, 60 and 120 min than in the same cells exposed to 21% O2 . The agent also accumulated in hypoxic tumors in vivo to give a tumor/muscle ratio (T/M) of 5.0 ± 1.2 (n = 3) as measured by PET at 2 h post-injection (p.i.). This was further confirmed by ex vivo biodistribution data. In addition, [(68) Ga]-HP-DO3A-NI displayed very favorable pharmacokinetic properties, as it was cleared largely through the kidneys with little to no accumulation in liver, heart or lung (%ID/g HP-DO3A, which lacks the nitroimidazole moiety, and by PET imaging of tumor-bearing mice breathing air versus 100% O2 . Given the commercial availability of cGMP (68) Ge/(68) Ga generators and the ease of (68) Ga labeling, the new agent could potentially be widely applied for imaging tumor hypoxia prior to radiation therapy. Copyright © 2015 John Wiley & Sons, Ltd.

  11. A testis-specific and testis developmentally regulated tumor protein D52 (TPD52)-like protein TPD52L3/hD55 interacts with TPD52 family proteins

    International Nuclear Information System (INIS)

    Cao Qinhong; Chen Jie; Zhu Li; Liu Yun; Zhou Zuomin; Sha Jiahao; Wang Shui; Li Jianmin

    2006-01-01

    Tumor protein D52-like proteins (TPD52) are small coiled-coil motif bearing proteins that were first identified in breast cancer. TPD52 and related proteins have been implicated in cell proliferation, apoptosis, and vesicle trafficking. To date, three human TPD52 members had been identified, named hD52 (TPD52), hD53 (TPD52L1), and hD54 (TPD52L2). The most important characteristic of the protein family is a highly conserved coiled-coil motif that is required for homo- and heteromeric interaction with other TPD52-like proteins. Herein, we identified a novel TPD52-like sequence (TPD52L3, or hD55) in human testis using cDNA microarray. Sequence analysis of the deduced protein suggests that hD55 contains a coiled-coil motif and is highly conserved compared with other TPD52-like sequences. Yeast two-hybrid and GST pull-down assays revealed that hD55 interacts with hD52, hD53, hD54, and itself. cDNA microarray detection found that hD55 was expressed at 5.6-fold higher levels in adult testis than in fetal testis. Additionally, the expression profile shows that hD55 is testis-specific, indicating a potential role for hD55 in testis development and spermatogenesis

  12. Efficacy and safety of two polyherbal combinations: E-MA-H and E-MA-HP in male sexual dysfunction.

    Science.gov (United States)

    Kulkarni, Mugdha P; Shinde, Bhakti S; Chaudhari, Manoj Kumar V; Avhad, Ganesh M; Pensalwar, Shrikant V; Prasad, B S V; Deshpande, Manoj Kumar M; Rosenbloom, Richard A

    2011-01-01

    Efficacy and safety of 2 herbal products--E-MA-H at 2 dose levels, low (HLD) and high (HHD), and E-MA-HP (HP) capsules--versus placebo (PL) was evaluated in subjects with male sexual dysfunction. Males aged 21-60 with erectile dysfunction, premature ejaculation, or other form of sexual dysfunction were studied in this triple-blind, randomized, placebo-controlled, parallel-groups trial. Subjects received any one of the following 4 interventions: E-MA-H 2 capsules at night (HLD) for 60 days; E-MA-H 2 capsules twice daily for 30 days, followed by 2 capsules at night for 30 days (HHD); E-MA-HP (HP) 2 capsules twice daily for 60 days; or placebo (PL) 2 capsules twice daily for 60 days. All dosage regimens were standardized to 2 capsules twice daily by using 2 matching placebo capsules as the morning dose for HLD and on days 31-60 for HHD. Efficacy outcome measures were the international index of erectile function; index for premature ejaculation; erectile dysfunction inventory of treatment satisfaction; subjects' and investigators' global assessment. Safety was assessed through adverse events; hematology; blood chemistry. Of 148 subjects enrolled, 1 was excluded from analysis; data on the intention-to-treat population of 147 (PL = 36, HLD = 38, HHD = 37, HP = 36) were analyzed. There was a significant (P HP (18.55 ± 1.36) compared with PL (6.83 ± 1.52). The same pattern was seen with increase in index for premature ejaculation scores: HLD (9.68 ± 1.17), HHD (10.27 ± 1.05), HP (11.36 ± 1.20) versus PL (3.77 ± 1.04). There was no significant difference in effect among the active treatment groups. The incidence of adverse events was similar in all the groups. Laboratory evaluations did not show any clinically significant abnormality in any of the groups. Treatment with HLD, HHD, and HP is well tolerated, and more effective than placebo (P < 0.01), in subjects with erectile dysfunction, premature ejaculation, and other forms of sexual dysfunction.

  13. The Maastricht Frailty Screening Tool for Hospitalised Patients (MFST-HP) to Identify Non-Frail Patients.

    Science.gov (United States)

    Warnier, Ron M J; van Rossum, Erik; van Kuijk, Sander M J; Mulder, Wubbo J; Schols, Jos M G A; Kempen, Gertrudis I J M

    2017-09-01

    The Maastricht frailty screening tool for hospitalised patients (MFST-HP) is a frailty screening tool that is fully integrated in the nursing assessment at admission. This study aims to determine the predictive value of the MFST-HP for the health outcomes length of hospital stay, discharge destination, readmission and mortality. Data of 2691 hospitalised patients (70+), admitted between 01-01-2013 and 31-12-2013, were included in the study. The predictive value of the MFST-HP was analysed by means of receiver operating characteristics curves. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) for different MFST-HP cut-off scores were examined. Mean age of the population was 78.9 years (SD 6.4) and their average length of stay was 10.2 days (SD 9.7). Nearly 75.0% of the patients were discharged to their home and around. Approximately 25% of the patients were readmitted within 120 days. Mortality rates were 4.3% and 9.5% (within 30 or 120 days postdischarge, respectively). The area under the curve was moderate and varied from 0.50 to 0.69 for the different outcomes. As a result of high values on negative predictive value (between 73.5% and 96.7%) the MFST-HP is able to rule out a large proportion of non-frail patients. In this study 84% of the patients had a MFST-HP score of ≥ 6, suggested as most favourable cut off. The MFST-HP seems to operate more strongly as a non-frailty indicator than as a frailty indicator and may in this respect help professionals to decide upon subsequent care. The MFST-HP is able to rule out 84% of the non-frail population in this study. The remaining 16% need to be assessed by means of a comprehensive geriatric assessment or rapid geriatric assessment, to gain more insight in the level of vulnerability in the frail-group. © 2017 John Wiley & Sons Ltd.

  14. Calculation of conversion coefficients Hp(3)/K air using the PENELOPE Monte Carlo code and comparison with MCNP calculation results

    International Nuclear Information System (INIS)

    Daures, J.; Gouriou, J.; Bordy, J.M.

    2010-01-01

    The authors report calculations performed using the MNCP and PENELOPE codes to determine the Hp(3)/K air conversion coefficient which allows the Hp(3) dose equivalent to be determined from the measured value of the kerma in the air. They report the definition of the phantom, a 20 cm diameter and 20 cm high cylinder which is considered as representative of a head. Calculations are performed for an energy range corresponding to interventional radiology or cardiology (20 keV-110 keV). Results obtained with both codes are compared

  15. Analyzing powers in the dd→3Hen(3Hp) reactions at intermediate energies

    International Nuclear Information System (INIS)

    Ladygin, V. P.; Kiselev, A. S.; Kurilkin, A. K.; Vasiliev, T. A.; Isupov, A. Yu.; Ladygina, N. B.; Malakhov, A. I.; Reznikov, S. G.; Uesaka, T.; Saito, T.; Hatano, M.; Kato, H.; Sakoda, S.; Uchigashima, N.; Yako, K.; Janek, M.; Maeda, Y.; Nishikawa, J.; Ohnishi, T.; Sakamoto, N.

    2008-01-01

    Data for the deuteron analyzing powers in the dd→ 3 Hen( 3 Hp) reactions obtained at 140-270 MeV are discussed. The observed negative sign of the tensor analyzing powers A yy , A xx and A xz at small angles clearly demonstrate the sensitivity to the ratio of the D and S state components of the 3 He wave function. The behavior of the tensor analyzing powers at backward angles is sensitive to the short-range spin structure of the deuteron. However, the one-nucleon exchange calculations using standard 3 He and deuteron wave functions fail to reproduce the strong variation of the tensor analyzing powers as a function of angle in the cms. Sensitivity to relativistic effects is also discussed

  16. T4 RIA precision profiles automatically computed using a small programmable pocket calculator HP-41 CV

    International Nuclear Information System (INIS)

    Ditu, M.; Waud, J.M.; Hamilton, R.G.; Wagner, H.N.

    1984-01-01

    Precision profiles as useful tool for assurance of assay quality in 10 independent T4 RIAs have been automatically obtained using a small programmable pocket calculator HP-41 CV. For each T4 assay batch, the within-assay coefficient of variation varied from 7 to 11% in the hormone concentration range of 2 to 20μg/dl. The difference in coefficient of variation for all the 10 successive assay batches of T4 never exceeded 1% in the same hormone concentrations regions. All the above findings demonstrate that the precision profile can be used as a powerful tool for assessing the assay quality and consistency of overall random error between successive assay batches [fr

  17. hp-version discontinuous Galerkin methods on polygonal and polyhedral meshes

    CERN Document Server

    Cangiani, Andrea; Georgoulis, Emmanuil H; Houston, Paul

    2017-01-01

    Over the last few decades discontinuous Galerkin finite element methods (DGFEMs) have been witnessed tremendous interest as a computational framework for the numerical solution of partial differential equations. Their success is due to their extreme versatility in the design of the underlying meshes and local basis functions, while retaining key features of both (classical) finite element and finite volume methods. Somewhat surprisingly, DGFEMs on general tessellations consisting of polygonal (in 2D) or polyhedral (in 3D) element shapes have received little attention within the literature, despite the potential computational advantages. This volume introduces the basic principles of hp-version (i.e., locally varying mesh-size and polynomial order) DGFEMs over meshes consisting of polygonal or polyhedral element shapes, presents their error analysis, and includes an extensive collection of numerical experiments. The extreme flexibility provided by the locally variable elemen t-shapes, element-sizes, and elemen...

  18. On the Realization of First-Order Current-Mode AP/HP Filter

    Directory of Open Access Journals (Sweden)

    W. Tangsrirat

    2013-12-01

    Full Text Available A compact circuit topology for the realization of the current-mode first-order allpass (AP and highpass (HP filters is described. The proposed circuit contains a minimum number of components, i.e., eight bipolar transistors and one grounded capacitor. The advantages of this circuit are the use of only grounded capacitor as a passive element, the electronic tunability of its parameters and its potential for low-voltage operation. Some simulation results are also reported, which demonstrate the effectiveness of the proposed circuit. Owing to the pole frequency of the filter circuit is normally dependent on temperature; a low-voltage translinear–based current source circuit for temperature compensation is also suggested.

  19. An hp-local Discontinuous Galerkin Method for Parabolic Integro-Differential Equations

    KAUST Repository

    Pani, Amiya K.

    2010-06-06

    In this article, a priori error bounds are derived for an hp-local discontinuous Galerkin (LDG) approximation to a parabolic integro-differential equation. It is shown that error estimates in L 2-norm of the gradient as well as of the potential are optimal in the discretizing parameter h and suboptimal in the degree of polynomial p. Due to the presence of the integral term, an introduction of an expanded mixed type Ritz-Volterra projection helps us to achieve optimal estimates. Further, it is observed that a negative norm estimate of the gradient plays a crucial role in our convergence analysis. As in the elliptic case, similar results on order of convergence are established for the semidiscrete method after suitably modifying the numerical fluxes. The optimality of these theoretical results is tested in a series of numerical experiments on two dimensional domains. © 2010 Springer Science+Business Media, LLC.

  20. Plasma disruption forces: a program for the HP 9845 and 9872 B plotter

    International Nuclear Information System (INIS)

    Foss, M.H.

    1982-11-01

    A computer program is described for the calculation of currents, forces, torques, and voltages in the conducting first wall and limiter of a tokamak reactor experiencing a plasma quench. Instructions are given for running the program on a HP 9845 B desk-top computer with a 9872 B plotter. The first wall and limiter have been modelled by 44 coaxial loops. The inductance matrix among the loops is calculated, and the currents in them are calculated step-by-step over time, driven by the flux from the decaying plasma current and subject to the field from the plasma, from the poloidal field coils, and from all current loops. An insulating break in the limiter or first wall can be modelled by imposing a back voltage which causes all the currents in the limiter or first wall to sum to zero. This potential is related to the voltage between segments of a segmented limiter or first wall

  1. Orbital HP-Clouds for Solving Schr?dinger Equation inQuantum Mechanics

    Energy Technology Data Exchange (ETDEWEB)

    Chen, J; Hu, W; Puso, M

    2006-10-19

    Solving Schroedinger equation in quantum mechanics presents a challenging task in numerical methods due to the high order behavior and high dimension characteristics in the wave functions, in addition to the highly coupled nature between wave functions. This work introduces orbital and polynomial enrichment functions to the partition of unity for solution of Schroedinger equation under the framework of HP-Clouds. An intrinsic enrichment of orbital function and extrinsic enrichment of monomial functions are proposed. Due to the employment of higher order basis functions, a higher order stabilized conforming nodal integration is developed. The proposed methods are implemented using the density functional theory for solution of Schroedinger equation. Analysis of several single and multi-electron/nucleus structures demonstrates the effectiveness of the proposed method.

  2. Test report for run-in acceptance testing of Project W-151 300 HP mixing pumps

    International Nuclear Information System (INIS)

    Berglin, B.G.

    1998-01-01

    This report documents the results of a performance demonstration and operational checkout of three 300 HP mixer pumps in accordance with WHC-SD-WI51-TS-001 ''Mixer Pump Test Specification for Project W-151'' and Statement of Work 8K520-EMN-95-004 ''Mixer Pump Performance Demonstration at MASF'' in the 400 Area Maintenance and Storage Facility (MASF) building. Testing of the pumps was performed by Fast Flux Test Facility (FFTF) Engineering and funded by the Tank Waste Remediation System (TWRS) Project W-151. Testing began with the first pump on 04-01-95 and ended with the third pump on 11-01-96. Prior to testing, the MASF was modified and prepared to meet the pump testing requirements set forth by the Test Specification and the Statement of Work

  3. Crystallography and Morphology of Niobium Carbide in As-Cast HP-Niobium Reformer Tubes

    Science.gov (United States)

    Buchanan, Karl G.; Kral, Milo V.

    2012-06-01

    The microstructures of two as-cast heats of niobium-modified HP stainless steels were characterized. Particular attention was paid to the interdendritic niobium-rich carbides formed during solidification of these alloys. At low magnifications, these precipitates are grouped in colonies of similar lamellae. Higher magnifications revealed that the lamellae actually obtain two distinct morphologies. The type I morphology exhibits broad planar interfaces with a smooth platelike shape. Type II lamellae have undulating interfaces and an overall reticulated shape. To provide further insight into the origin of these two different morphologies, the microstructure and crystallography of each have been studied in detail using high resolution scanning electron microscopy, transmission electron microscopy, various electron diffraction methods (electron backscatter diffraction (EBSD), selected area diffraction (SAD), and convergent beam electron diffraction (CBED)), and energy dispersive X-ray spectroscopy.

  4. Programs for data processing in radioimmunoassay using the HP-41C programmable calculator

    International Nuclear Information System (INIS)

    1981-09-01

    The programs described provide for analysis, with the Hewlett Packard HP-41C calculator, of counting data collected in radioimmunoassays or other related in-vitro assays. The immediate reason for their development was to assist laboratories having limited financial resources and serious problems of quality control. The programs are structured both for ''off-line'' use, with manual entry of counting data into the calculator through the keyboard, and, in a slightly altered version, for ''on-line'' use, with automatic data entry from an automatic well scintillation counter originally designed at the IAEA. Only the off-line variant of the programs is described. The programs determine from appropriate counting data the concentration of analyte in unknown specimens, and provide supplementary information about the reliability of these results and the consistency of current and past assay performance

  5. Urinary 1-hydroxypyrene (1-HP) in environmental and occupational studies--a review

    DEFF Research Database (Denmark)

    Hansen, Ase M; Mathiesen, Line; Pedersen, Marie

    2008-01-01

    in petrochemical industries among coke-oven workers. Occupational PAH exposure was the major factor determining high urinary concentration of 1-HP. The highest concentrations were observed among workers in the petrochemical industry at work places in Taiwan and China. To evaluate environmental and low......A total of 132 studies were identified, of which 25 studies addressed environmental exposure to polycyclic aromatic hydrocarbons (PAHs) in the general population. Of these 9 studies included children. Of 101 studies among workers in various occupations, 32 studies were in petrochemical industries......, 29 studies in foundries, 14 studies in asphalt work, 21 studies working in urban air, 6 studies in combustion, 3 studies in soil remediation, and 2 studies in printing. Environmental and occupational studies were identified through an extensive search of the PubMed database up to November 2006...

  6. Monolithic discretization of linear thermoelasticity problems via adaptive multimesh hp-FEM

    Czech Academy of Sciences Publication Activity Database

    Šolín, Pavel; Červený, Jakub; Dubcová, Lenka; Andrš, David

    2010-01-01

    Roč. 234, č. 7 (2010), s. 2350-2357 ISSN 0377-0427 R&D Projects: GA ČR(CZ) GA102/07/0496; GA AV ČR IAA100760702 Institutional research plan: CEZ:AV0Z20570509 Keywords : linear elasticity * monolithic discretization * adaptive multimesh hp-FEM Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering Impact factor: 1.029, year: 2010 http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6TYH-4X1J73B-V-11&_cdi=5619&_user=640952&_pii=S0377042709005731&_origin=search&_coverDate=08%2F01%2F2010&_sk=997659992&view=c&wchp=dGLzVzb-zSkWA&md5=3665f1549355544e9c36e84a4adfd086&ie=/sdarticle.pdf

  7. Analysis of Regolith Properties Using Seismic Signals Generated by InSight's HP3 Penetrator

    Science.gov (United States)

    Kedar, Sharon; Andrade, Jose; Banerdt, Bruce; Delage, Pierre; Golombek, Matt; Grott, Matthias; Hudson, Troy; Kiely, Aaron; Knapmeyer, Martin; Knapmeyer-Endrun, Brigitte; Krause, Christian; Kawamura, Taichi; Lognonne, Philippe; Pike, Tom; Ruan, Youyi; Spohn, Tilman; Teanby, Nick; Tromp, Jeroen; Wookey, James

    2017-10-01

    InSight's Seismic Experiment for Interior Structure (SEIS) provides a unique and unprecedented opportunity to conduct the first geotechnical survey of the Martian soil by taking advantage of the repeated seismic signals that will be generated by the mole of the Heat Flow and Physical Properties Package (HP3). Knowledge of the elastic properties of the Martian regolith have implications to material strength and can constrain models of water content, and provide context to geological processes and history that have acted on the landing site in western Elysium Planitia. Moreover, it will help to reduce travel-time errors introduced into the analysis of seismic data due to poor knowledge of the shallow subsurface. The challenge faced by the InSight team is to overcome the limited temporal resolution of the sharp hammer signals, which have significantly higher frequency content than the SEIS 100 Hz sampling rate. Fortunately, since the mole propagates at a rate of ˜1 mm per stroke down to 5 m depth, we anticipate thousands of seismic signals, which will vary very gradually as the mole travels. Using a combination of field measurements and modeling we simulate a seismic data set that mimics the InSight HP3-SEIS scenario, and the resolution of the InSight seismometer data. We demonstrate that the direct signal, and more importantly an anticipated reflected signal from the interface between the bottom of the regolith layer and an underlying lava flow, are likely to be observed both by Insight's Very Broad Band (VBB) seismometer and Short Period (SP) seismometer. We have outlined several strategies to increase the signal temporal resolution using the multitude of hammer stroke and internal timing information to stack and interpolate multiple signals, and demonstrated that in spite of the low resolution, the key parameters—seismic velocities and regolith depth—can be retrieved with a high degree of confidence.

  8. Particle removal challenges of EUV patterned masks for the sub-22nm HP node

    Science.gov (United States)

    Rastegar, Abbas; Eichenlaub, Sean; Kadaksham, Arun John; Lee, Byunghoon; House, Matt; Huh, Sungmin; Cha, Brian; Yun, Henry; Mochi, Iacopo; Goldberg, Kenneth

    2010-04-01

    The particle removal efficiency (PRE) of cleaning processes diminishes whenever the minimum defect size for a specific technology node becomes smaller. For the sub-22 nm half-pitch (HP) node, it was demonstrated that exposure to high power megasonic up to 200 W/cm2 did not damage 60 nm wide TaBN absorber lines corresponding to the 16 nm HP node on wafer. An ammonium hydroxide mixture and megasonics removes >=50 nm SiO2 particles with a very high PRE. A sulfuric acid hydrogen peroxide mixture (SPM) in addition to ammonium hydroxide mixture (APM) and megasonic is required to remove >=28 nm SiO2 particles with a high PRE. Time-of-flight secondary ion mass spectroscopy (TOFSIMS) studies show that the presence of O2 during a vacuum ultraviolet (VUV) (λ=172 nm) surface conditioning step will result in both surface oxidation and Ru removal, which drastically reduce extreme ultraviolet (EUV) mask life time under multiple cleanings. New EUV mask cleaning processes show negligible or no EUV reflectivity loss and no increase in surface roughness after up to 15 cleaning cycles. Reviewing of defect with a high current density scanning electron microscope (SEM) drastically reduces PRE and deforms SiO2 particles. 28 nm SiO2 particles on EUV masks age very fast and will deform over time. Care must be taken when reviewing EUV mask defects by SEM. Potentially new particles should be identified to calibrate short wavelength inspection tools. Based on actinic image review, 50 nm SiO2 particles on top of the EUV mask will be printed on the wafer.

  9. Development of heterotopic transplantation of the testis with the epididymis to evaluate an aspect of testicular immunology in rats.

    Directory of Open Access Journals (Sweden)

    Kai Yi

    Full Text Available Transplantation of testicular cells and tissues has been studied for the investigation of immunology of the testis, which is an immunologically privileged organ. However, reports of transplant of the testis at organ level have been extremely limited because of technical difficulties of the orthotopic testis transplantation (OTT in experimental animals. In the present study, we developed a new and simple model of the heterotopic testis transplantation (HTT, which is donor testis transplantation into the cervical region of recipients, in a syngeneic model in rats [donor Lewis (LEW graft to LEW recipient]. The duration of HTT was significantly shorter and success rate higher than that of OTT. To histologically evaluate HTT, the local immune responses were compared among the syngeneic model, an acute rejection allogeneic model [donor Augustus Copenhagen Irish (ACI graft to LEW recipient] and a chronic rejection allogeneic model (donor F344 graft to LEW recipient at postoperative day 3. We found that allogeneic ACI grafts resulted in mild and not severe orchitic lesions, whereas immune responses of allogeneic F344 grafts seemed intact and were not significantly different from those of syngeneic LEW grafts. These results suggest that our new operative procedure will be useful in future for the investigation of the testicular immunology.

  10. Assessment of four calculation methods proposed by the EC for waste hazardous property HP 14 'Ecotoxic'.

    Science.gov (United States)

    Hennebert, Pierre; Humez, Nicolas; Conche, Isabelle; Bishop, Ian; Rebischung, Flore

    2016-02-01

    Legislation published in December 2014 revised both the List of Waste (LoW) and amended Appendix III of the revised Waste Framework Directive 2008/98/EC; the latter redefined hazardous properties HP 1 to HP 13 and HP 15 but left the assessment of HP 14 unchanged to allow time for the Directorate General of the Environment of the European Commission to complete a study that is examining the impacts of four different calculation methods for the assessment of HP 14. This paper is a contribution to the assessment of the four calculation methods. It also includes the results of a fifth calculation method; referred to as "Method 2 with extended M-factors". Two sets of data were utilised in the assessment; the first (Data Set #1) comprised analytical data for 32 different waste streams (16 hazardous (H), 9 non-hazardous (NH) and 7 mirror entries, as classified by the LoW) while the second data set (Data Set #2), supplied by the eco industries, comprised analytical data for 88 waste streams, all classified as hazardous (H) by the LoW. Two approaches were used to assess the five calculation methods. The first approach assessed the relative ranking of the five calculation methods by the frequency of their classification of waste streams as H. The relative ranking of the five methods (from most severe to less severe) is: Method 3>Method 1>Method 2 with extended M-factors>Method 2>Method 4. This reflects the arithmetic ranking of the concentration limits of each method when assuming M=10, and is independent of the waste streams, or the H/NH/Mirror status of the waste streams. A second approach is the absolute matching or concordance with the LoW. The LoW is taken as a reference method and the H wastes are all supposed to be HP 14. This point is discussed in the paper. The concordance for one calculation method is established by the number of wastes with identical classification by the considered calculation method and the LoW (i.e. H to H, NH to NH). The discordance is

  11. Infectivity of H7 LP and HP influenza viruses at different temperatures and pH and persistence of H7 HP virus in poultry meat at refrigeration temperature.

    Science.gov (United States)

    Beato, Maria Serena; Mancin, Marzia; Bertoli, Elena; Buratin, Alessandra; Terregino, Calogero; Capua, Ilaria

    2012-11-25

    The aims of this study were to assess the infectivity of highly pathogenic (HP) and low pathogenicity (LP) H7 AI viruses at different temperatures and pH values and to investigate the persistance of HP H7 virus in chicken, turkey and duck meat. The H7 viruses tested remained infectious at +4°C and +20°C for 200 and >50 days, respectively. At pH 5, H7 viruses retained their infectivity for a shorter period of time compared to pH 7. The infectivity of HP H7 was detected >2 months in meat maintained at +4°C and was higher in chicken meat compared to turkey and duck meat. Results of this study show that higher temperatures and lower pH values both reduce virus infectivity and demonstrate that HP H7 virus can remain infectious in meat for extended periods of time. Copyright © 2012 Elsevier Inc. All rights reserved.

  12. Serum concentrations of haptoglobin and haptoglobin-matrix metalloproteinase 9 (Hp-MMP 9) complexes of bovine calves in a bacterial respiratory challenge model.

    Science.gov (United States)

    Hanthorn, Christy J; Dewell, Grant A; Dewell, Renee D; Cooper, Vickie L; Wang, Chong; Plummer, Paul J; Lakritz, Jeffrey

    2014-12-06

    Serum haptoglobin (Hp) and haptoglobin matrix metalloproteinase 9 complexes (Hp-MMP 9) have been identified as biomarkers with diagnostic potential in cattle with conditions resulting in an acute inflammatory response. The purpose of this study was to evaluate potential diagnostic applications of serum Hp and Hp-MMP 9 concentrations in calves with BRD and establish a timeline for their detection in calves experimentally challenged with Bibersteinia trehalosi and Mannheimia haemolytica. Thirty-five cross bred dairy calves were inoculated via tracheal catheterization with either a PCR confirmed leukotoxin negative B. trehalosi isolate, a PCR confirmed leukotoxin positive B. trehalosi isolate, a Mannheimia haemolytica isolate, a combination of leukotoxin negative B. trehalosi and M. haemolytica, or a negative control. Serum samples were collected throughout the study. Calves were euthanized and necropsy performed on day 10 post inoculation. M. haemolytica inoculated calves had increased lung involvement. Serum Hp and Hp- MMP 9 concentrations were elevated compared to the other treatment groups. Increases in serum Hp and Hp-MMP 9 concentrations for the M. haemolytica group were significantly different from other study groups on day 7 of the study. B. trehalosi inoculated calves did not have increased lung involvement compared to control calves, but the leukotoxin positive B. trehalosi group demonstrated increased serum Hp-MMP 9 concentrations from day 3 to the end of the study compared to the pre-inoculation concentrations. Serum Hp-MMP 9 concentration is a useful diagnostic tool for detecting early pulmonary inflammation in calves challenged with B. trehalosi and M. haemolytica. Serum Hp-MMP 9 may also be a useful tool in detecting subclinical pulmonary inflammation in challenged calves.

  13. Agonistic anti-CD40 promotes early development and increases the incidence of severe thyroid epithelial cell hyperplasia (TEC H/P) in CD4-/- mice.

    Science.gov (United States)

    Yu, Shiguang; Downey, Edward F; Braley-Mullen, Helen

    2013-10-01

    IFN-γ-/-NOD.H-2h4 mice develop thyroid epithelial cell hyperplasia (TEC H/P) characterised by abnormal proliferation of thyrocytes and infiltration of thyroids by CD4+ and CD8+ T cells, macrophages and dendritic cells. CD8+ T cells from mice with severe TEC H/P transfer similar lesions to SCID recipients, whereas CD4+ T cells transfer mild TEC H/P. CD4- and CD8- deficient IFN-γ-/-NOD.H-2h4 mice were generated to determine if CD4+ T cells were required for initial activation of the CD8+ T cells that transfer TEC H/P. After 6-8 months on NaI water, only 2 of 60 CD8-/- mice developed severe TEC H/P, whereas 31 of 101 CD4-/- mice developed severe TEC H/P and fibrosis comparable in severity to that of IFN-γ-/- mice. However, splenocytes from CD4-/- mice with severe TEC H/P did not effectively transfer severe TEC H/P to SCID recipients. When CD4-/- donors were given agonistic anti-CD40 mAb, most developed severe TEC H/P and their cells transferred severe TEC H/P to SCID recipients. These results indicate that agonistic anti-CD40 can provide an important signal for activation of autoreactive CD8+ T cells that transfer severe TEC H/P. Therefore, targeting or blocking CD40 could provide effective therapy for diseases involving hyperplasia and fibrosis mediated by CD8+ T cells.

  14. Subcellular distribution of di-(2-ethylhexyl)phthalate in rat testis

    International Nuclear Information System (INIS)

    Ono, Hiroshi; Saito, Yoshiaki; Imai, Kiyoshi

    2004-01-01

    Subcellular distribution of di-(2-ethylhexyl)phthalate (DEHP) in the testis was studied by single oral administration of [3,4,5,6- 3 H]-phthalic acid di-(2-ethylhexyl) ester (DEHP- 3 H) or phthalic acid di-(2-ethyl[1- 3 H]hexyl) ester ( 3 H-DEHP) to 8-week-old male rats. Autoradiographs and electron microscopic autoradiographs were prepared from the testis, liver and kidney at 6 and 24 hr after administration and distribution of radioactive materials in the tissues were observed. In the autoradiographic specimen at 6 hr after administration of DEHP 3 H-labeled at phthalic acid moiety (DEHP- 3 H), many grains were observed in the testis, mainly at the basal area of seminiferous tubules at the stages IX to I of the spermatogenic cycle. Electron microscopic autoradiographs taken at the same time revealed that localization of grains were in the smooth-surfaced endoplasmic reticulum and mitochondria of Sertoli cells. A few grains were also present at the Golgi apparatus and lysosome of Sertoli cells, and at the interfaces between the Sertoli cells or between Sertoli cells and spermatocytes, and in the cytoplasm of spermatocytes. Autoradiographs of the liver revealed grains in the centrilobular hepatocytes, localized at mitochondria, rough-surfaced endoplasmic reticulum and peroxisomes. In the kidney, the radioactivity was localized at the brush border of the tubular cells in the pars recta of proximal tubules. In the 24-hr specimen, the grain density in the seminiferous tubules obviously decreased. On the other hand, by autoradiography with DEHP 3 H-labeled at the alcohol ( 3 H-DEHP), only a few grains were observed in autoradiographs of the testes at 6 hr after administration. No grains were noted in autoradiographs of the liver and kidney with 3 H-DEHP. The results showed that the phthalic acid ester was splitted rapidly in the body and only the phthalic acid moiety distributed into the cells. (author)

  15. DUX4 binding to retroelements creates promoters that are active in FSHD muscle and testis.

    Directory of Open Access Journals (Sweden)

    Janet M Young

    2013-11-01

    Full Text Available The human double-homeodomain retrogene DUX4 is expressed in the testis and epigenetically repressed in somatic tissues. Facioscapulohumeral muscular dystrophy (FSHD is caused by mutations that decrease the epigenetic repression of DUX4 in somatic tissues and result in mis-expression of this transcription factor in skeletal muscle. DUX4 binds sites in the human genome that contain a double-homeobox sequence motif, including sites in unique regions of the genome as well as many sites in repetitive elements. Using ChIP-seq and RNA-seq on myoblasts transduced with DUX4 we show that DUX4 binds and activates transcription of mammalian apparent LTR-retrotransposons (MaLRs, endogenous retrovirus (ERVL and ERVK elements, and pericentromeric satellite HSATII sequences. Some DUX4-activated MaLR and ERV elements create novel promoters for genes, long non-coding RNAs, and antisense transcripts. Many of these novel transcripts are expressed in FSHD muscle cells but not control cells, and thus might contribute to FSHD pathology. For example, HEY1, a repressor of myogenesis, is activated by DUX4 through a MaLR promoter. DUX4-bound motifs, including those in repetitive elements, show evolutionary conservation and some repeat-initiated transcripts are expressed in healthy testis, the normal expression site of DUX4, but more rarely in other somatic tissues. Testis expression patterns are known to have evolved rapidly in mammals, but the mechanisms behind this rapid change have not yet been identified: our results suggest that mobilization of MaLR and ERV elements during mammalian evolution altered germline gene expression patterns through transcriptional activation by DUX4. Our findings demonstrate a role for DUX4 and repetitive elements in mammalian germline evolution and in FSHD muscular dystrophy.

  16. Protective effects of Launaea procumbens on rat testis damage by CCl4

    Directory of Open Access Journals (Sweden)

    Khan Rahmat

    2012-08-01

    Full Text Available Abstract Background Traditionally various human diseases of kidneys, hormonal imbalance and sexual diseases are treated with Launaea procumbens (L. In the present study protective effects of methanolic extract of Launaea procumbens (LPME was evaluated against CCl4-induced oxidative damages in rat testis. Methods To examine the protective effects of Launaea procumbens on testis against oxidative stress of carbon tetrachloride in male rat, 30 male albino rats were equally divided into 5 groups (6 rats. First group was given standard diet and drinking water. Second group received CCl4 3 ml/kg intraperitoneally (30% in olive oil. Third and forth were given orally 100; 200 mg/kg b.w., in 99.8% dimethyl sulphooxide (DMSO, Launaea procumbens methanolic extracts (LPME after 48 h of CCl4 treatment twice a week and sixth group received only LPME in DMSO at a dose of 200 mg/kg b.w., for four weeks. Protective effects of Launaea procumbens were observed on sperm concentration, motility and morphology, serum reproductive hormonal level, activity of antioxidant enzymes, lipid peroxidation (TBARS and DNA damages. Results Results of the present study revealed that treatment of CCl4 significantly (p  reduced sperm concentration and motility comparatively to controls. Level of testosterone, luteinizing hormone and follicle stimulating hormone, were depleted markedly (p with treatment of CCl4. In addition, CCl4 induction in rats reduced activities of antioxidant enzymes while increased lipid peroxidation and DNA damages. Co-administration of LPME significantly (p improved these alterations in improving of hormonal level, activities of antioxidant enzymes and lipid peroxidation near to control rats. Conclusion From the results it is suggested that Launaea procumbens methanolic extract has the ability to protect testis against oxidative damages, possibly through antioxidant effects of its bioactive compounds.

  17. Mechanisms of the Testis Toxicity Induced by Chronic Exposure to Mequindox

    Directory of Open Access Journals (Sweden)

    Qianying Liu

    2017-09-01

    Full Text Available Mequindox (MEQ is a synthetic antimicrobial agent widely used in China since the 1980s. Although the toxicity of MEQ is well recognized, its testis toxicity has not been adequately investigated. In the present study, we provide evidence that MEQ triggers oxidative stress, mitochondrion dysfunction and spermatogenesis deficiency in mice after exposure to MEQ (0, 25, 55, and 110 mg/kg in the diet for up to 18 months. The genotoxicity and adrenal toxicity may contribute to sperm abnormalities caused by MEQ. Moreover, using LC/MS-IT-TOF analysis, two metabolites, 3-methyl-2-(1-hydroxyethyl quinoxaline-N4-monoxide (M4 and 3-methyl-2-(1-hydroxyethyl quinoxaline-N1-monoxide (M8, were detected in the serum of mice, which directly confirms the relationship between the N→O group reduction metabolism of MEQ and oxidative stress. Interestingly, only M4 was detected in the testes, suggesting that the higher reproductive toxicity of M4 than M8 might be due to the increased stability of M4-radical (M4-R compared to M8-radical (M8-R. Furthermore, the expression of the blood-testis barrier (BTB-associated junctions such as tight junctions, gap junctions and basal ectoplasmic specializations were also examined. The present study demonstrated for the first time the role of the M4 in testis toxicity, and illustrated that the oxidative stress, mitochondrion dysfunction and interference in spermatogenesis, as well as the altered expression of BTB related junctions, were involved in the reproductive toxicity mediated by MEQ in vivo.

  18. Identification of cancer/testis-antigen genes by massively parallel signature sequencing

    Science.gov (United States)

    Chen, Yao-Tseng; Scanlan, Matthew J.; Venditti, Charis A.; Chua, Ramon; Theiler, Gregory; Stevenson, Brian J.; Iseli, Christian; Gure, Ali O.; Vasicek, Tom; Strausberg, Robert L.; Jongeneel, C. Victor; Old, Lloyd J.; Simpson, Andrew J. G.

    2005-01-01

    Massively parallel signature sequencing (MPSS) generates millions of short sequence tags corresponding to transcripts from a single RNA preparation. Most MPSS tags can be unambiguously assigned to genes, thereby generating a comprehensive expression profile of the tissue of origin. From the comparison of MPSS data from 32 normal human tissues, we identified 1,056 genes that are predominantly expressed in the testis. Further evaluation by using MPSS tags from cancer cell lines and EST data from a wide variety of tumors identified 202 of these genes as candidates for encoding cancer/testis (CT) antigens. Of these genes, the expression in normal tissues was assessed by RT-PCR in a subset of 166 intron-containing genes, and those with confirmed testis-predominant expression were further evaluated for their expression in 21 cancer cell lines. Thus, 20 CT or CT-like genes were identified, with several exhibiting expression in five or more of the cancer cell lines examined. One of these genes is a member of a CT gene family that we designated as CT45. The CT45 family comprises six highly similar (>98% cDNA identity) genes that are clustered in tandem within a 125-kb region on Xq26.3. CT45 was found to be frequently expressed in both cancer cell lines and lung cancer specimens. Thus, MPSS analysis has resulted in a significant extension of our knowledge of CT antigens, leading to the discovery of a distinctive X-linked CT-antigen gene family. PMID:15905330

  19. Expression of orexin B and its receptor 2 in rat testis.

    Science.gov (United States)

    Liguori, Giovanna; Pavone, Luigi Michele; Assisi, Loredana; Langella, Emilia; Tafuri, Simona; Mirabella, Nicola; Costagliola, Anna; Vittoria, Alfredo

    2017-02-01

    The peptides orexin A (OxA) and orexin B (OxB) deriving from a common precursor molecule, prepro-orexin, by proteolytic cleavage, bind the two G-coupled OX 1 and OX 2 receptors. While OX 1 selectively binds OxA, OX 2 shows similar affinity for both orexins. Firstly discovered in the hypothalamus, orexins and their receptors have been found in other brain regions as well as in peripheral tissues of mammals, thus resulting involved in the regulation of a broad variety of physiological functions. While the functional localization of OxA and OX 1 in the mammalian genital tract has been already described, the expression of OxB and OX 2 and their potential role in the reproductive functions remain to be explored. Here, we investigated the presence of OxB and OX 2 in the rat testis by immunohistochemical and biochemical analyses. The results definitely demonstrated the localization of OxB and OX 2 in pachytene and second spermatocytes as well as in spermatids at all stages of the cycle of the seminiferous epithelium. The expression of both OX 2 mRNA and protein in the rat testis was also established by RT-PCR and Western blotting, respectively. The analysis of the molecular mechanism of action of OxB in the rat testis showed that OxB, in contrast with OxA, is unable to promote steroidogenesis. These results translate into the regulation of diverse biological actions by OxA and OxB in the male gonad. Copyright © 2015 Elsevier Inc. All rights reserved.

  20. Extreme divergence of Wolbachia tropism for the stem-cell-niche in the Drosophila testis.

    Science.gov (United States)

    Toomey, Michelle E; Frydman, Horacio M

    2014-12-01

    Microbial tropism, the infection of specific cells and tissues by a microorganism, is a fundamental aspect of host-microbe interactions. The intracellular bacteria Wolbachia have a peculiar tropism for the stem cell niches in the Drosophila ovary, the microenvironments that support the cells producing the eggs. The molecular underpinnings of Wolbachia stem cell niche tropism are unknown. We have previously shown that the patterns of tropism in the ovary show a high degree of conservation across the Wolbachia lineage, with closely related Wolbachia strains usually displaying the same pattern of stem cell niche tropism. It has also been shown that tropism to these structures in the ovary facilitates both vertical and horizontal transmission, providing a strong selective pressure towards evolutionary conservation of tropism. Here we show great disparity in the evolutionary conservation and underlying mechanisms of stem cell niche tropism between male and female gonads. In contrast to females, niche tropism in the male testis is not pervasive, present in only 45% of niches analyzed. The patterns of niche tropism in the testis are not evolutionarily maintained across the Wolbachia lineage, unlike what was shown in the females. Furthermore, hub tropism does not correlate with cytoplasmic incompatibility, a Wolbachia-driven phenotype imprinted during spermatogenesis. Towards identifying the molecular mechanism of hub tropism, we performed hybrid analyses of Wolbachia strains in non-native hosts. These results indicate that both Wolbachia and host derived factors play a role in the targeting of the stem cell niche in the testis. Surprisingly, even closely related Wolbachia strains in Drosophila melanogaster, derived from a single ancestor only 8,000 years ago, have significantly different tropisms to the hub, highlighting that stem cell niche tropism is rapidly diverging in males. These findings provide a powerful system to investigate the mechanisms and evolution of

  1. The short term effects of resveratrol on ischemia-reperfusion injury in rat testis.

    Science.gov (United States)

    Yuluğ, Esin; Türedi, Sibel; Karagüzel, Ersagun; Kutlu, Omer; Menteşe, Ahmet; Alver, Ahmet

    2014-03-01

    The purpose of this study was to identify changes taking place in the rat testis at the 24th hour of reperfusion following testicular torsion and to evaluate the effects of resveratrol (RSV), a powerful antioxidant, in preventing these changes using novel biochemical parameters and histopathology. Eighteen adult male rats were divided into three groups: Sham-operated (S), torsion/detorsion (T/D), and T/D+RSV groups. In the T/D group, testicular ischemia was achieved by rotating the left testis 720° clockwise for 4h. In the T/D+RSV group, 20mg/kg RSV was administered intraperitoneally 30 min before detorsion. All rats were sacrificed 24h after detorsion. Serum and tissue malondialdehyde (MDA) concentrations, ischemia modified albumin (IMA), total oxidative status (TOS), total antioxidant status (TAS), oxidative stress index (OSI), and histopathological damage score were analyzed. Serum MDA, IMA, TOS, and OSI levels rose significantly in the T/D group. Serum MDA and IMA values were lower in the T/D+RES groups, but not significantly. OSI and TOS values were lower in the T/D+RES group, and the difference was significant. TAS values decreased significantly in the T/D group and rose in the T/D+RSV group, but not significantly. Ipsilateral tissue MDA values were significantly elevated in the T/D group and decreased in the T/D+RSV group, but not significantly. Apoptosis and histopathological damage increased significantly in the T/D group and decreased significantly in the T/D+RSV group. In the contralateral testis, apoptosis increased significantly in the T/D group. It decreased significantly in the T/D+RSV group. Our findings show that RSV had a protective effect against oxidative damage induced with a testicular T/D model, especially at the antiapoptotic and histopathological level. OSI may be a good guide to the clinical status of testicular T/D. © 2014.

  2. Survey of Growth Hormone Effects on Methotrexate Side Effects in Rat Testis

    Directory of Open Access Journals (Sweden)

    A. Khaki

    2007-10-01

    Full Text Available Introduction & Objectives: Methotrexate (MTX is a chemotherapeutic agent that used for the treatment of a variety of tumors and inflammatory diseases. The aim of this study was planed to see the role of human growth hormone (hGH on testis recovery after treatment with MTX in rat. Materials & Methods: In this experimental study the fifty male wistar rat were selected and randomly divided into five groups; control (n=10 and test (n=40. Hormone grope was received 0.3mg/kg hGH (IP for 28 consequence day, drug group was received 1mg/kg MTX (IP per week for four consequence week, protective group was received 0.3mg/kg hGH (IP for 28 consequence day plus1mg/kg MTX (IP per week for four consequence week and treatment group was received 1mg/kg MTX (IP per week for two consequence week with 0.3mg/kg hGH (IP for14 consequence day, however the control group just received vehicle (IP. In 14 and 28 day the testis tissue of rat in whole groups were removed and sperm was collected from epididymis then prepared for analysis. The statically analysis was ANOVA test. Results: This study was confirmed MTX had destructive effects in testis germinal cells and rate of spermatogenesis was decreased (P<0.05 as compared with control group, and HGh had treatment effects in degenerative seminiferous epithelium and improve spermatogenesis (P<0.05 as compared in other groups.Conclusion: Since in our study hGH had recovery effect on spermatogenesis cycle after treatment with MTX, and it can not have useful effect if only prescript in chemotherapy period than consequently hGH inducement is suitable for sperm health ability parameters after chemotherapy in patient.

  3. Towards a rational terminology in the study of the gubernaculum testis: arguments in support of the notion that the cremasteric sac should be considered the gubernaculum in postnatal rats and other mammals

    NARCIS (Netherlands)

    P. van der Schoot

    1996-01-01

    textabstractThere is need for a consistent definition of structures caudal to the testis that variously are termed 'gubernaculum testis' as a basis for understanding the emergence and sexually dimorphic further growth and differentiation of this specifically mammalian

  4. Expression and function analysis of metallothionein in the testis of Portunus trituberculatus exposed to cadmium

    Energy Technology Data Exchange (ETDEWEB)

    Xiang, Dong-Fang [School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211 (China); The Sperm Laboratory, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058 (China); Zhu, Jun-Quan; Jin, Shan [School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211 (China); Hu, Yan-Jun [Department of Reproductive Endocrinology, Women' s Hospital, School of Medicine, Zhejiang University, Hangzhou 310006 (China); Tan, Fu-Qing [The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003 (China); Yang, Wan-Xi, E-mail: wxyang@spermlab.org [The Sperm Laboratory, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058 (China)

    2013-09-15

    Highlights: •We identified P. trituberculatus MT-1 and MT-2 complete cDNA sequence. •We analyzed the protein alignment comparisons and phylogenetic trees of MT-1 and MT-2. •RT-PCR analysis the tissue expression of MT-1 and MT-2 mRNA. •The spatial and temporal distribution pattern of MT-1 and MT-2 mRNA during spermiogenesis. •Testis MT-1 and MT-2 mRNA expression are dramatically affected after the cadmium exposure. -- Abstract: Metallothioneins (MTs) possess a unique molecular structure that provides metal-binding and redox capabilities. These capabilities include the maintenance of metal equilibria that protect against heavy metals (especially cadmium) and oxidative damage. Past studies have focused on the function of MTs in vertebrates. However, the functions of MTs during spermiogenesis in invertebrates remain unclear. In order to investigate the function of MTs during spermiogenesis in Portunus trituberculatus, we used RT-PCR and RACE to identify two MT complete cDNA sequences in the total RNA from the P. trituberculatus testis. The 450 bp MT-1 cDNA consists of a 77 bp 5′ untranslated region, a 196 bp 3′ untranslated region, and a 177 bp open reading frame that encodes 58 amino acids including 19 cysteines. The 581 bp MT-2 cDNA consists of 73 bp 5′ untranslated region, a 328 bp 3′ untranslated region, and a 180 bp open reading frame that encodes 59 amino acids including 18 cysteines. MT-1 and MT-2 of P. trituberculatus more closely resemble invertebrate (especially crab) MT homologues than vertebrate MT homologues as indicated by protein alignment comparisons and phylogenetic tree analysis. MT-1 and MT-2 were detected in the heart, testis, muscle, hepatopancreas, and gill of P. trituberculatus by tissue expression analysis. In addition, MT-1 and MT-2 are present during the entire process of spermiogenesis in P. trituberculatus as indicated by H and E staining and in situ hybridization. MT-1 and MT-2 expression levels significantly increase

  5. Validation of endogenous normalizing genes for expression analyses in adult human testis and germ cell neoplasms

    DEFF Research Database (Denmark)

    Svingen, T; Jørgensen, Anne; Rajpert-De Meyts, E

    2014-01-01

    The measurement of gene expression levels in cells and tissues typically depends on a suitable point of reference for inferring biological relevance. For quantitative (or real-time) RT-PCR assays, the method of choice is often to normalize gene expression data to an endogenous gene that is stably...... to define suitable normalizing genes for specific cells and tissues. Here, we report on the performance of a panel of nine commonly employed normalizing genes in adult human testis and testicular pathologies. Our analyses revealed significant variability in transcript abundance for commonly used normalizers...

  6. Adenomatous hyperplasia of the rete testis: A rare intrascrotal lesion managed with limited testicular excision.

    Science.gov (United States)

    Catanzariti, Francesco; Servi, Lucilla; Fabiani, Andrea; Filosa, Alessandra; Mammana, Gabriele

    2016-10-05

    Testicular cancer is one of the most frequent in young men and its incidence is increasing in recent years because of incidental finding during routine ultrasound exams. Adenomatous hyperplasia of the rete testis is one of the benign and rare pathological types incidentally detected and very few cases are described in the literature. A 40 years old man come to our attention for a balanoposthitis without testicular pain. During andrological examination we performed palpation of the testes and we noticed a palpable nodule of hard consistency in the left testicle. We then performed an ultrasound exam of the testis which highlighted the presence of an intra-didymus neoformation with diameters of 1.2 x 1.6 cm and with the presence of cysts inside. We also performed blood tests to check tumor markers alpha fetoprotein, beta hCG and LDH which resulted inside the normal range. We then conducted a chest and abdomen CT scan that showed no pathological elements. Therefore, as we suspected that this tumor was benign, we performed an enucleation of the neoplasm. The definitive histological examination revealed the presence of dilated ducts lined with epithelial cubic-columnar cells with clear cytoplasm rich in glycogen and the pathologist so concluded that the tumor could be classified as adenomatous hyperplasia of the rete testis. At three months of follow up, the patient doesn't have any recurrent lesion to either testicles. Adenomatous hyperplasia of the rete testis is a very rare intrascrotal lesion. This histological type is the most frequent between benign lesion of the ovary, but few works in literature reported this histological type in the male gonad and, in most of these works, authors described these lesion at epididymis. We believe that a conservative approach must be considered mandatory in case of testicular lesions 1.5 cm in diameter. A radical approach might have alterate fertility of the patient and also have caused psychological trauma more than an enucleation

  7. Adenomatous hyperplasia of the rete testis: A rare intrascrotal lesion managed with limited testicular excision

    Directory of Open Access Journals (Sweden)

    Francesco Catanzariti

    2016-10-01

    Full Text Available Introduction: Testicular cancer is one of the most frequent in young men and its incidence is increasing in recent years because of incidental finding during routine ultrasound exams. Adenomatous hyperplasia of the rete testis is one of the benign and rare pathological types incidentally detected and very few cases are described in the literature. Case report: A 40 years old man come to our attention for a balanoposthitis without testicular pain. During andrological examination we performed palpation of the testes and we noticed a palpable nodule of hard consistency in the left testicle. We then performed an ultrasound exam of the testis which highlighted the presence of an intra-didymus neoformation with diameters of 1.2 x 1.6 cm and with the presence of cysts inside. We also performed blood tests to check tumor markers alpha fetoprotein, beta hCG and LDH which resulted inside the normal range. We then conducted a chest and abdomen CT scan that showed no pathological elements. Therefore, as we suspected that this tumor was benign, we performed an enucleation of the neoplasm. The definitive histological examination revealed the presence of dilated ducts lined with epithelial cubic-columnar cells with clear cytoplasm rich in glycogen and the pathologist so concluded that the tumor could be classified as adenomatous hyperplasia of the rete testis. At three months of follow up, the patient doesn’t have any recurrent lesion to either testicles. Discussion: Adenomatous hyperplasia of the rete testis is a very rare intrascrotal lesion. This histological type is the most frequent between benign lesion of the ovary, but few works in literature reported this histological type in the male gonad and, in most of these works, authors described these lesion at epididymis. Conclusion: We believe that a conservative approach must be considered mandatory in case of testicular lesions 1.5 cm in diameter. A radical approach might have alterate fertility of the

  8. An unexpected finding of a rare intrascrotal lesion: the sertoliform cystadenoma of the rete testis.

    Science.gov (United States)

    Sahnan, Kapil; Manjunath, Aditya; Vaughan-Shaw, Peter G; Mitsopoulos, Grigorios

    2013-02-20

    A 19-year-old man presented with a right testicular swelling. Testicular ultrasound demonstrated areas suspicious for malignancy and so it was decided to proceed for a right radical inguinal orchiectomy. Initial histological examination revealed a multiloculated cystic lesion at the area of the rete testis yet normal testicular parenchyma elsewhere. Specialist histological opinion was sought from the regional teratoma multi-disciplinary team which confirmed the diagnosis of a sertoliform cystadenoma, an extremely rare benign testicular neoplasm. The recognition of the benign nature of the mass enabled complete reassurance to be offered to the patient and avoided further oncological treatment.

  9. Metastatic Transitional Cell Carcinoma of the Bladder to the Testis: A Case Report

    Directory of Open Access Journals (Sweden)

    Gregory N. Kozak

    2012-01-01

    Full Text Available An 84-year-old gentleman presented with onset of gross hematuria in September 2010. Follow-up investigations revealed T1 superficially invasive, poorly differentiated, papillary urothelial carcinoma. He subsequently had GreenLight laser for BPH and bladder neck contracture on two occasions. He developed a right hydrocele 16 months after initial presentation and during his hydrocelectomy, a rock-hard right epididymis and testicle were discovered. Pathology revealed metastatic urothelial carcinoma replacing nearly the entire testis with lymphovascular invasion.

  10. Expression and localization of N- and E-cadherin in the human testis and epididymis

    DEFF Research Database (Denmark)

    Andersson, A M; Edvardsen, K; Skakkebaek, N E

    1994-01-01

    Cellular interactions in the testis and epididymis are an important prerequisite for spermatogenesis and sperm maturation, and involve a well-developed complex of intercellular junctions. Cadherins are cell surface proteins which mediate intercellular Ca(2+)-dependent adhesion and are believed...... epididymis E-cadherin, but not N-cadherin, was expressed and localized to the surface of the principal epithelial cells as shown by immunohistochemistry. These observations indicate that cadherins play an important role in the organization of the seminiferous and epididymal epithelium....

  11. Malignant peritoneal mesothelioma associated with deep vein thrombosis following radiotherapy for seminoma of the testis

    International Nuclear Information System (INIS)

    Sato, Fuminori; Yamazaki, Hajime; Ataka, Ken; Mashima, Ichiro; Suzuki, Kenta; Takahashi, Toru; Umezu, Hajime; Gejyo, Fumitake

    2000-01-01

    A 52-year-old man developed malignant peritoneal mesothelioma 17 years after radiotherapy for seminoma of the testis. Although asbestos exposure is considered to be the major risk factor for the development of malignant mesothelioma, prior therapeutic radiation has also been postulated as a causative factor. The unexplained appearance of ascites or pleural effusion within a previously irradiated area should be considered suggestive of malignant mesothelioma in any long-term survivor of cancer. In addition, the patient suffered a deep vein thrombosis four years before the diagnosis of mesothelioma. Deep vein thrombosis is a common complication of malignant disease, and is often the first clue to occult malignancy. (author)

  12. Malignant peritoneal mesothelioma associated with deep vein thrombosis following radiotherapy for seminoma of the testis

    Energy Technology Data Exchange (ETDEWEB)

    Sato, Fuminori; Yamazaki, Hajime; Ataka, Ken; Mashima, Ichiro; Suzuki, Kenta; Takahashi, Toru; Umezu, Hajime; Gejyo, Fumitake [Niigata Univ. (Japan). School of Medicine

    2000-11-01

    A 52-year-old man developed malignant peritoneal mesothelioma 17 years after radiotherapy for seminoma of the testis. Although asbestos exposure is considered to be the major risk factor for the development of malignant mesothelioma, prior therapeutic radiation has also been postulated as a causative factor. The unexplained appearance of ascites or pleural effusion within a previously irradiated area should be considered suggestive of malignant mesothelioma in any long-term survivor of cancer. In addition, the patient suffered a deep vein thrombosis four years before the diagnosis of mesothelioma. Deep vein thrombosis is a common complication of malignant disease, and is often the first clue to occult malignancy. (author)

  13. Expression and localization of nerve growth factor (NGF in the testis of alpaca (llama pacos

    Directory of Open Access Journals (Sweden)

    Changsheng Dong

    2011-04-01

    Full Text Available During alpaca testis development and spermatogenesis, nerve growth factor (NGF may play an important role. The main aim of this study was to determine the expression and localization of NGF in the alpaca testis, and to discuss the important role of NGF in alpaca reproductive characteristics. Immunohistochemical staining technique and real-time PCR were used. The expression of NGF in the same cells one-month old (newborn alpacas 12-month, and 24-month old alpacas showed significant differences (p < 0.05; 12- and 24-month old alpacas showed no significant differences (p > 0.05; NGF at different cell stages showed no significant differences (p > 0.05. It suggests that NGF may be involved in the regulation of spermatogenesis, which provides direct evidence for NGF action in the alpaca testis during postnatal development and spermatogenesis. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 1, pp. 55–61

  14. SOX9 regulates microRNA miR-202-5p/3p expression during mouse testis differentiation

    DEFF Research Database (Denmark)

    Wainwright, Elanor N; Jorgensen, Joan S; Kim, Youngha

    2013-01-01

    MicroRNAs are important regulators of developmental gene expression, but their contribution to fetal gonad development is not well understood. We have identified the evolutionarily conserved gonadal microRNAs miR-202-5p and miR-202-3p as having a potential role in regulating mouse embryonic gonad...... differentiation. These microRNAs are expressed in a sexually dimorphic pattern as the primordial XY gonad differentiates into a testis, with strong expression in Sertoli cells. In vivo, ectopic expression of pri-miR-202 in XX gonads did not result in molecular changes to the ovarian determination pathway...... findings indicate that expression of the conserved gonad microRNA, miR-202-5p/3p, is downstream of the testis-determining factor SOX9, suggesting an early role in testis development....

  15. Endometrioid like yolk sac tumor of the testis with small teratomatous foci: A case report and review of the literature.

    Science.gov (United States)

    Hazarika, Prabir

    2015-01-01

    I have reported a case of endometrioid like yolk sac tumor of the testis in a 20-year-old boy. Endometrioid like yolk sac tumor is a rare tumor. A few cases have been reported in ovary. In case of male, a case of pure glandular endometrioid like yolk sac tumor is reported in a 43 years male in right undescended testis and another case of abdominal metastasis showing endometrioid pattern from mixed testicular germ cell tumor comprising of teratoma and embryonal carcinoma. My patient was a 20-year-old male presented with painless enlargement of right testis. Grossly the tumor was glistening creamish white with a multicystic appearance. Histopathological examination showed the tumor to be composed of glandular elements resembling early secretory endometrium, foci of keratinized thin squamous epithelium and a single focus of benign cartilage. The glandular elements show immunohistochemical positivity for AFP, cytokeratin 7 (CK7) and epithelial membrane antigen (EMA).

  16. Endometrioid like yolk sac tumor of the testis with small teratomatous foci: A case report and review of the literature

    Directory of Open Access Journals (Sweden)

    Prabir Hazarika

    2015-01-01

    Full Text Available I have reported a case of endometrioid like yolk sac tumor of the testis in a 20-year-old boy. Endometrioid like yolk sac tumor is a rare tumor. A few cases have been reported in ovary. In case of male, a case of pure glandular endometrioid like yolk sac tumor is reported in a 43 years male in right undescended testis and another case of abdominal metastasis showing endometrioid pattern from mixed testicular germ cell tumor comprising of teratoma and embryonal carcinoma. My patient was a 20-year-old male presented with painless enlargement of right testis. Grossly the tumor was glistening creamish white with a multicystic appearance. Histopathological examination showed the tumor to be composed of glandular elements resembling early secretory endometrium, foci of keratinized thin squamous epithelium and a single focus of benign cartilage. The glandular elements show immunohistochemical positivity for AFP, cytokeratin 7 (CK7 and epithelial membrane antigen (EMA.

  17. Nuclear levels and patterns of histone H3 modification and HP1 proteins after inhibition of histone deacetylases

    Czech Academy of Sciences Publication Activity Database

    Bártová, Eva; Pacherník, J.; Harničarová, Andrea; Kovařík, Aleš; Kovaříková, Martina; Hofmanová, Jiřina; Skalníková, M.; Kozubek, Michal; Kozubek, Stanislav

    2005-01-01

    Roč. 118, č. 21 (2005), s. 5035-5046 R&D Projects: GA AV ČR(CZ) 1QS500040508; GA ČR(CZ) GA202/04/0907 Institutional research plan: CEZ:AV0Z50040507 Keywords : histone H3 * HDAC inhibitors * HP1 proteins Subject RIV: BO - Biophysics

  18. Inhibitory effects of rHP-NAP IgY against Helicobacter pylori attachment to AGS cell line.

    Science.gov (United States)

    Borhani, Katayoun; Mohabati Mobarez, Ashraf; Khabiri, Ali Reza; Behmanesh, Mehrdad; Khoramabadi, Nima

    2016-08-01

    Helicobacter pylori is a major human pathogen related to gastric adenocarcinoma and gastroduodenal diseases. Treatment of H. pylori infections is complicated by the rise of antibiotic resistance, necessitating investigation of alternative therapies. One such alternative is passive immunization by oral administration of antibacterial immunoglobulin. In the present study, chicken immunoglobulin (IgY) was used for passive immunotherapy against a major virulence factor of H. pylori, namely recombinant HP-Nap protein. Recombinant HP-Nap was prepared and used to immunize hens. IgY was purified from the eggs by polyethylene glycol precipitation method with a total IgY-HP-NAP yield of 30 mg per egg. The inhibitory effect of specific IgY on H. pylori attachment was investigated in AGS cell line infected by the bacteria. The results demonstrate the potent effect of IgY- HP-NAP in inhibition of H. pylori attachment to the AGS cells. Copyright © 2016 Elsevier Ltd. All rights reserved.

  19. An adaptive hp-DG method with dynamically-changing meshes for non-stationary compressible Euler equations

    Czech Academy of Sciences Publication Activity Database

    Korous, L.; Šolín, Pavel

    2013-01-01

    Roč. 95, č. 1 (2013), S425-S444 ISSN 0010-485X Institutional support: RVO:61388998 Keywords : numerical simulation * finite element method * hp-adaptivity Subject RIV: BA - General Math ematics Impact factor: 1.055, year: 2013

  20. Optimization of statistical methods for HpGe gamma-ray spectrometer used in wide count rate ranges

    Energy Technology Data Exchange (ETDEWEB)

    Gervino, G., E-mail: gervino@to.infn.it [UNITO - Università di Torino, Dipartimento di Fisica, Turin (Italy); INFN - Istituto Nazionale di Fisica Nucleare, Sez. Torino, Turin (Italy); Mana, G. [INRIM - Istituto Nazionale di Ricerca Metrologica, Turin (Italy); Palmisano, C. [UNITO - Università di Torino, Dipartimento di Fisica, Turin (Italy); INRIM - Istituto Nazionale di Ricerca Metrologica, Turin (Italy)

    2016-07-11

    The need to perform γ-ray measurements with HpGe detectors is a common technique in many fields such as nuclear physics, radiochemistry, nuclear medicine and neutron activation analysis. The use of HpGe detectors is chosen in situations where isotope identification is needed because of their excellent resolution. Our challenge is to obtain the “best” spectroscopy data possible in every measurement situation. “Best” is a combination of statistical (number of counts) and spectral quality (peak, width and position) over a wide range of counting rates. In this framework, we applied Bayesian methods and the Ellipsoidal Nested Sampling (a multidimensional integration technique) to study the most likely distribution for the shape of HpGe spectra. In treating these experiments, the prior information suggests to model the likelihood function with a product of Poisson distributions. We present the efforts that have been done in order to optimize the statistical methods to HpGe detector outputs with the aim to evaluate to a better order of precision the detector efficiency, the absolute measured activity and the spectra background. Reaching a more precise knowledge of statistical and systematic uncertainties for the measured physical observables is the final goal of this research project.

  1. LATE TRIASSIC OBLIQUE EXTRUSION OF UHP/HP COMPLEXES IN THE ATBASHI ACCRETIONARY COMPLEX OF SOUTH TIANSHAN, KYRGYZSTAN

    Directory of Open Access Journals (Sweden)

    Wenjiao Xiao

    2017-01-01

    Full Text Available The exhumation and tectonic emplacement of eclogites and blueschists take place in forearc accretionary complexes by either forearc- or backarc-directed extrusion, but few examples have been well analysed in detail. Here we present an example of oblique wedge extrusion of UHP/HP rocks in the Atbashi accretionary complex of the Kyrgyz South Tianshan.

  2. Optimization of statistical methods for HpGe gamma-ray spectrometer used in wide count rate ranges

    Science.gov (United States)

    Gervino, G.; Mana, G.; Palmisano, C.

    2016-07-01

    The need to perform γ-ray measurements with HpGe detectors is a common technique in many fields such as nuclear physics, radiochemistry, nuclear medicine and neutron activation analysis. The use of HpGe detectors is chosen in situations where isotope identification is needed because of their excellent resolution. Our challenge is to obtain the "best" spectroscopy data possible in every measurement situation. "Best" is a combination of statistical (number of counts) and spectral quality (peak, width and position) over a wide range of counting rates. In this framework, we applied Bayesian methods and the Ellipsoidal Nested Sampling (a multidimensional integration technique) to study the most likely distribution for the shape of HpGe spectra. In treating these experiments, the prior information suggests to model the likelihood function with a product of Poisson distributions. We present the efforts that have been done in order to optimize the statistical methods to HpGe detector outputs with the aim to evaluate to a better order of precision the detector efficiency, the absolute measured activity and the spectra background. Reaching a more precise knowledge of statistical and systematic uncertainties for the measured physical observables is the final goal of this research project.

  3. [Correlation Study on Chinese Medical Syndrome Types of Chronic Atrophic Gastritis Patients, Hp, and IL-1β Polymorphism].

    Science.gov (United States)

    Zhang, Jian-zhi; Feng, Quan-lin; Hu, Yi-lu; Yang, Ti; Zhang, Ying

    2015-12-01

    To explore the correlation between Chinese medical (CM) syndrome types of chronic atrophic gastritis (CAG) patients and Helicobacter pylori (Hp) infection, polymorphisms of IL-1B, and IL-1β. Totally 192 CAG patients and 202 healthy subjects (as the healthy control group) were recruited in this case-control study. The Hp infection was tested by 13C-urea breath test and colloidal gold-labeled assay (GICA). The concentration of peripheral blood IL-1β was measured by ELISA. The polymorphisms of IL-1B gene in the promoter region were analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Pi-Wei weakness syndrome (PWWS) was dominant in CAG patients (31.77%, 61/192 cases). The Hp infection ratio in CAG patients was 53.65% (103/192 cases), of which, Pi-Wei damp-heat syndrome(PWDHS, 64.86%, 24/37 cases) and Gan-Wei disharmony syndrome (GWDS, 66.67%, 24/36 cases) were dominant. Compared with the health control group, the plasma concentration of IL-1β was obviously elevated in CAG patients with PWDHS, GWDS, and static blood obstructing collaterals syndrome (SBOCS) (all P 0.05). The incidence risk of CAG was not associated with IL-1B polymorphism. But CM syndrome types of CAG patients was associated with Hp infection and peripheral blood IL-1β levels.

  4. Research on the mechanism of HP mediated PI3K/AKT/GSK3β pathways in gastric cancer.

    Science.gov (United States)

    Geng, W; Zhang, H-Y

    2017-07-01

    This study aimed to investigate the mechanism of Helicobacter pylori (HP) mediated PI3K/AKT/GSK3β signal pathways in the occurrence of gastric cancer. For this purpose, a total of 90 patients were selected; 30 were suffering from gastric cancer and gastric ulcer (observation group I), 30 were suffering from pure gastric ulcer (observation group II) and 30 were with chronic gastritis (control group), and the lesion tissues were extracted by using endoscopy. Then, rapid urease test, RT-PCR and Western blot tests were conducted to detect the positivity rate of HP infection, the expression levels of PI3K/AKT/GSK3β mRNA and protein respectively. The results showed that the positivity rate of HP infection, expression level of the PI3K/AKT/GSK3β mRNA and protein from the samples of the observation group I were significantly higher than other two groups (p-value HP may mediate the PI3K/AKT/GSK3β signal pathways in the occurrence of gastric cancer.

  5. A role for MeCP2 in switching gene activity via chromatin unfolding and HP1γ displacement.

    Directory of Open Access Journals (Sweden)

    Maartje C Brink

    Full Text Available Methyl-CpG-binding protein 2 (MeCP2 is generally considered to act as a transcriptional repressor, whereas recent studies suggest that MeCP2 is also involved in transcription activation. To gain insight into this dual function of MeCP2, we assessed the impact of MeCP2 on higher-order chromatin structure in living cells using mammalian cell systems harbouring a lactose operator and reporter gene-containing chromosomal domain to assess the effect of lactose repressor-tagged MeCP2 (and separate MeCP2 domains binding in living cells. Our data reveal that targeted binding of MeCP2 elicits extensive chromatin unfolding. MeCP2-induced chromatin unfolding is triggered independently of the methyl-cytosine-binding domain. Interestingly, MeCP2 binding triggers the loss of HP1γ at the chromosomal domain and an increased HP1γ mobility, which is not observed for HP1α and HP1β. Surprisingly, MeCP2-induced chromatin unfolding is not associated with transcriptional activation. Our study suggests a novel role for MeCP2 in reorganizing chromatin to facilitate a switch in gene activity.

  6. 75 FR 41531 - Hewlett Packard (HP) Global Product Development, Working On-Site at General Motors Corporation...

    Science.gov (United States)

    2010-07-16

    ... DEPARTMENT OF LABOR Employment and Training Administration [TA-W-73,488] Hewlett Packard (HP) Global Product Development, Working On-Site at General Motors Corporation, Milford, MI; Notice of Revised Termination of Investigation Pursuant to Section 223 of the Trade Act of 1974, as amended, an investigation...

  7. Host-guest kinetic interactions between HP-β-cyclodextrin and drugs for prediction of bitter taste masking.

    Science.gov (United States)

    Guo, Zhen; Wu, Fei; Singh, Vikramjeet; Guo, Tao; Ren, Xiaohong; Yin, Xianzhen; Shao, Qun; York, Peter; Patterson, Laurence H; Zhang, Jiwen

    2017-06-05

    Cyclodextrins (CD) are widely used bitter taste masking agents, for which the binding equilibrium constant (K) for the drug-CD complex is a conventional parameter for quantitating the taste masking effects. However, some exceptions have been reported to the expected relationship between K and bitterness reduction and the relationship between kinetic parameters of a drug-CD interaction, including association rate constant (K a ) and disassociation rate constant (K d ), and taste masking remains unexplored. In this study, based upon a database of kinetic parameters of drugs-HP-β-CD generated by Surface Plasmon Resonance Imaging for 485 drugs, the host-guest kinetic interactions between drugs and HP-β-CD for prediction of taste masking effects have been investigated. The taste masking effects of HP-β-CD for 13 bitter drugs were quantitatively determined using an electronic gustatory system (α-Astree e-Tongue). Statistical software was used to establish a model based on Euclidean distance measurements, K a and K d of the bitter drugs/HP-β-CD-complexes (R 2 =0.96 and Pkinetics of drug-CD interactions and taste masking was established and providing a new strategy for predicting the cyclodextrin mediated bitter taste masking. Copyright © 2017 Elsevier B.V. All rights reserved.

  8. Smart Dual-Mode Heat Pump With HP2Grid Functionality To Support Large-Scale Integration Of Intermittent Renewables

    DEFF Research Database (Denmark)

    Carmo, Carolina; Blarke, Morten Boje

    2013-01-01

    and compare the core technology options that are involved in the design of this concept, including high-pressure heat pumps, advanced thermal storage materials, and small-scale Rankine cycles with natural working fluids. The new concept – called HP2Grid - effectively represents state-of-the-art in terms...

  9. Scoring the home falls and accidents screening tool for health professionals (HOME FAST-HP): Evidence from one epidemiological study.

    Science.gov (United States)

    Mackenzie, Lynette; Byles, Julie

    2018-03-30

    Falls in older people are a major public health concern. To target falls prevention interventions, screening tools need to be able to identify older people at greater risk of falling. This study aimed to investigate the screening capacity of the Home Falls and Accidents Screening Tool for health professionals (HOME FAST-HP), and to identify the best cut-off score to identify older people at higher risk of falls using the HOME FAST-HP. The study used cross-sectional data from a random sample of 650 women from the 1921 to 1926 cohort of the Australian Longitudinal Study of Women's Health (ALSWH). Selected women were sent a postal survey including the HOME FAST-HP, falls history, and other health factors. Scores on the home fast were calculated and the cut-point for optimal sensitivity and specificity of the HOME FAST-HP in relation to falls was assessed using a Receiver Operating Characteristic curve. A total of 567 older women participated (response rate 87%). The mean age of participants was 77.5 yrs (95% CI 77.31-77.70). A total of 153 participants (27%) reported a fall in the previous six months. The mean number of hazards using the HOME FAST-HP was 9.74 (95% CI 9.48-10.01), range 2-22. Non-fallers had a mean of 9.6 hazards (95% CI 9.32-9.91) and fallers had a mean of 10.63 hazards (95% CI 10.08-11.19) which was a significant difference (t = 3.41, P = 0.001). The area under the receiver operator curve (AUC) was 0.58 (95% CI 0.53-0.64). A HOME FAST-HP cut-off score of 9 was associated with the optimal sensitivity for falls (73.9%), with specificity (37.9%), and positive predictive value was 30.6% and negative predictive value was 79.7%. The HOME FAST-HP can be used as a screening tool to identify fallers with a cut-off score of nine indicating a higher risk of falling. © 2018 Occupational Therapy Australia.

  10. Analysis of GAGE, NY-ESO-1 and SP17 cancer/testis antigen expression in early stage non-small cell lung carcinoma

    DEFF Research Database (Denmark)

    Gjerstorff, Morten F; Pøhl, Mette; Olsen, Karen E

    2013-01-01

    NSCLC has shown promising results. However, little is known about the expression of other cancer/testis antigens in NSCLC. In the present study the expression of cancer/testis antigens GAGE, NY-ESO-1 and SP17 was investigated in patients with completely resected, early stage, primary NSCLC....

  11. Emilio Pasquini, ed., Studi e problemi di critica testuale: 1960-2010. Per i 150 anni della Commissione per i testi di lingua

    Directory of Open Access Journals (Sweden)

    Giuseppe Di Stefano

    2016-01-01

    Full Text Available Reseña de Emilio Pasquini, ed., Studi e problemi di critica testuale: 1960-2010. Per i 150 anni della Commissione per i testi di lingua, Commissione per i testi di lingua (Collezione di opere inedite o rare, 169, Bologna, 2012, x + 320 pp. ISBN: 9788898096152.

  12. Effect of a long-chained fructan Raftiline HP on blood lipids and spontaneous atherosclerosis in low density receptor knockout mice

    DEFF Research Database (Denmark)

    Mortensen, Alicja; Poulsen, Morten; Frandsen, Henrik Lauritz

    2002-01-01

    The effect of a long-chained fructan Raftiline HP on spontaneous hypercholesterolemia and atherosclerosis was studied in 40 LDLR-/- male mice receiving isocaloric, balanced in fat content, purified diets with 0 or 10% Raftiline HP, for 16 weeks. The feed intake was comparable (3.9 v. 3.8 g/day) b...

  13. QTL list: qHP-PU-A3a [PGDBj Registered plant list, Marker list, QTL list, Plant DB link and Genome analysis methods[Archive

    Lifescience Database Archive (English)

    Full Text Available sphorus condition ... em1ge34b ... ChrA03 29.9 3.21 ... 10.1007/s00122-010-1301-1 20217384 ... QT91755 Brassica napus Brassicaceae qHP-PU-A3a LP and HP constitutive constitutive QTL for low and high pho

  14. QTL list: qHP-RVol-A3a [PGDBj Registered plant list, Marker list, QTL list, Plant DB link and Genome analysis methods[Archive

    Lifescience Database Archive (English)

    Full Text Available ve QTL for low and high phosphorus condition ... ChrA03 33.3 5.58 ... 10.1007/s00122-010-1301-1 20217384 ... QT91784 Brassica napus Brassicaceae qHP-RVol-A3a LP and HP constitutive constituti

  15. QTL list: qHP-DW-A3a [PGDBj Registered plant list, Marker list, QTL list, Plant DB link and Genome analysis methods[Archive

    Lifescience Database Archive (English)

    Full Text Available sphorus condition ... em1ge34b ... ChrA03 34.7 3.62 ... 10.1007/s00122-010-1301-1 20217384 ... QT91756 Brassica napus Brassicaceae qHP-DW-A3a LP and HP constitutive constitutive QTL for low and high pho

  16. QTL list: qHP-RL-C2a [PGDBj Registered plant list, Marker list, QTL list, Plant DB link and Genome analysis methods[Archive

    Lifescience Database Archive (English)

    Full Text Available sphorus condition ... sR6293 ... ChrC02 45.7 4.46 ... 10.1007/s00122-010-1301-1 20217384 ... QT91791 Brassica napus Brassicaceae qHP-RL-C2a LP and HP constitutive constitutive QTL for low and high pho

  17. QTL list: qHP-DW-C2a [PGDBj Registered plant list, Marker list, QTL list, Plant DB link and Genome analysis methods[Archive

    Lifescience Database Archive (English)

    Full Text Available sphorus condition ... sR6293 ... ChrC02 44.8 3.67 ... 10.1007/s00122-010-1301-1 20217384 ... QT91789 Brassica napus Brassicaceae qHP-DW-C2a LP and HP constitutive constitutive QTL for low and high pho

  18. QTL list: qHP-RSA-A3a [PGDBj Registered plant list, Marker list, QTL list, Plant DB link and Genome analysis methods[Archive

    Lifescience Database Archive (English)

    Full Text Available e QTL for low and high phosphorus condition ... em1ge34b ... ChrA03 31.7 6.32 ... 10.1007/s00122-010-1301-1 20217384 ... QT91757 Brassica napus Brassicaceae qHP-RSA-A3a LP and HP constitutive constitutiv

  19. QTL list: qHP-PU-C2a [PGDBj Registered plant list, Marker list, QTL list, Plant DB link and Genome analysis methods[Archive

    Lifescience Database Archive (English)

    Full Text Available sphorus condition ... ChrC02 42 3.72 ... 10.1007/s00122-010-1301-1 20217384 ... QT91790 Brassica napus Brassicaceae qHP-PU-C2a LP and HP constitutive constitutive QTL for low and high pho

  20. Mantle wedge exhumation beneath the Dora-Maira (U)HP dome unravelled by local earthquake tomography (Western Alps)

    Science.gov (United States)

    Solarino, Stefano; Malusà, Marco G.; Eva, Elena; Guillot, Stéphane; Paul, Anne; Schwartz, Stéphane; Zhao, Liang; Aubert, Coralie; Dumont, Thierry; Pondrelli, Silvia; Salimbeni, Simone; Wang, Qingchen; Xu, Xiaobing; Zheng, Tianyu; Zhu, Rixiang

    2018-01-01

    In continental subduction zones, the behaviour of the mantle wedge during exhumation of (ultra)high-pressure [(U)HP] rocks provides a key to distinguish among competing exhumation mechanisms. However, in spite of the relevant implications for understanding orogenic evolution, a high-resolution image of the mantle wedge beneath the Western Alps is still lacking. In order to fill this gap, we perform a detailed analysis of the velocity structure of the Alpine belt beneath the Dora-Maira (U)HP dome, based on local earthquake tomography independently validated by receiver function analysis. Our results point to a composite structure of the mantle wedge above the subducted European lithosphere. We found that the Dora-Maira (U)HP dome lays directly above partly serpentinized peridotites (Vp 7.5 km/s; Vp/Vs = 1.70-1.72), documented from 10 km depth down to the top of the eclogitized lower crust of the European plate. These serpentinized peridotites, possibly formed by fluid release from the subducting European slab to the Alpine mantle wedge, are juxtaposed against dry mantle peridotites of the Adriatic upper plate along an active fault rooted in the lithospheric mantle. We propose that serpentinized mantle-wedge peridotites were exhumed at shallow crustal levels during late Eocene transtensional tectonics, also triggering the rapid exhumation of (U)HP rocks, and were subsequently indented under the Alpine metamorphic wedge in the early Oligocene. Our findings suggest that mantle-wedge exhumation may represent a major feature of the deep structure of exhumed continental subduction zones. The deep orogenic levels here imaged by seismic tomography may be exposed today in older (U)HP belts, where mantle-wedge serpentinites are commonly associated with coesite-bearing continental metamorphic rocks.